LOCUS       DASQQQ010000001         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_165403, whole genome shotgun sequence.
ACCESSION   DASQQQ010000001 DASQQQ010000000
VERSION     DASQQQ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165403"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VE646_00005"
     CDS             complement(<1..346)
                     /locus_tag="VE646_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013286742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZD78414.1"
                     /translation="MEYRTLGGTGLQVSTHCLGAMMFGEWGNPDVDECVRIVHAALDA
                     GVNFVDTADVYSHGTSEEIVGRALRGRRDDVVLATKVHGEMGSGRNERGNSRVWITRE
                     VEDSLSRLGTDHI"
     gene            501..2024
                     /locus_tag="VE646_00010"
     CDS             501..2024
                     /locus_tag="VE646_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HZD78415.1"
                     /translation="MSVVLDGSSLTIEQLVRIARGNEPVELDPGALDRVRACRAMLEE
                     KLGAREIMYGTNTGIGEFSEVVLTDDQVERFQRFLVYNHAAGIGEPAPLEVVRAAMAS
                     RANVLAKGHSGVRPQIALTLAEMLNRGVTPVVCQKGSVGASGDLAPMAQIALLLLGEG
                     EAFFQGVRLPGRLALEQAGIEVPGLLARDGLGTINGSNVLTAMSALILYDVDRWLRQA
                     EIAAAMSLEALLANLKPYDVRVHELRGFPGAIRSAKAILRCIEGSDLQTGKIRTKVQD
                     AYSMRSTPQVIGAAHDAVAYARSQVEIELNGVGDNPLYLPEAKLTLTGANFQGTPVSL
                     PMEMVGQAVTMVSVLSERRLNRLTNPALSQGLPDFLTPDPGFFSGLMLSQYTADTLIT
                     EQRILSSPASVMSIPAAADQEDFVSMGMNTAIKNGQILDNAYAVIGIELMAAAQALDF
                     RDFHPGKGVQVAHEVVRRHVEHLDQDRPLHVDHDRVKALVASGEVLEAVESAVGPLS"
BASE COUNT          327 a          741 c          756 g          337 t
ORIGIN      
        1 cgatgtggtc ggtgccgagg cggctgaggc tgtcctcgac ctcccgggtg atccacacgc
       61 gggagttgcc acgctcgttc ctgcccgatc ccatctcccc gtggaccttg gtggccaaga
      121 ccacgtcgtc ccgtcggccc cgcagggccc ggcccacgat ctcctccgat gtgccgtgcg
      181 agtacacgtc ggccgtgtcg acgaagttga ccccggcgtc caaagcggca tgcacgatgc
      241 ggacacactc gtccacgtcg gggttgcccc actcgccgaa catcatcgct cccaggcaat
      301 gcgtgctgac ctgcaggccg gtcccaccca acgtccggta ctccatccgt gcctcctcga
      361 cgcgcttccc ccggcagtgt ctcgcagctg gcgcgggtcc gcgatgttcc cgcggggcgg
      421 ggggctttcg gccccgccct cggggttccg ggcgggccag catgcagctg acaccgagtc
      481 cacgggtgga aggagcagtc atgtcggtgg tcctggatgg ttcgtccctc acgatcgagc
      541 agctggttcg gatcgcccgg ggcaacgagc cggtggagct ggatcccggg gccctggacc
      601 gggtccgggc gtgccgggcg atgctggagg agaagctggg cgcccgcgag atcatgtacg
      661 gcaccaacac cgggatcggc gagttctccg aggtcgtcct gaccgacgac caggtggagc
      721 ggttccagcg cttcctggtc tacaaccacg ccgcgggcat cggtgagccc gctccgctcg
      781 aggtggtgcg ggccgccatg gccagccggg cgaacgtcct ggccaaggga cactcggggg
      841 tccgacccca gatcgcgctg acgctcgcgg agatgctgaa ccgaggcgtc accccggtcg
      901 tgtgccagaa gggctcggtg ggggccagcg gcgacctggc gccgatggcc cagatcgcgc
      961 tgctcctgct cggtgagggg gaggcgttct tccagggggt ccggctgccg gggcgtctgg
     1021 ccctcgagca ggccggcatc gaggtgccgg ggctcctggc gcgggacggg ctggggacga
     1081 tcaacggctc gaacgtgctc accgccatga gcgcactgat cctgtacgac gtggaccggt
     1141 ggctccgcca ggcggagatc gcggccgcga tgtccctcga ggccctgttg gccaacctga
     1201 agccgtacga cgtgcgcgtc cacgagctcc gcgggttccc cggcgcgatc cgcagcgcga
     1261 aggccatcct gcgctgcatc gagggcagcg acctgcagac cgggaagatc aggaccaagg
     1321 tccaggacgc ctactcgatg cgctccaccc cccaggtgat cggagcggcg cacgatgccg
     1381 tcgcgtacgc ccgctcgcag gtcgagatcg agctgaacgg cgtgggggac aaccccctgt
     1441 acctgccgga ggccaagctc acgctgaccg gcgcgaactt ccagggcaca ccggtctccc
     1501 tgccgatgga gatggtgggc caggcggtga cgatggtgag cgtgctttcc gagcggcggt
     1561 tgaaccgcct cacgaacccc gccctcagcc agggcctgcc cgatttcctc acgcccgatc
     1621 caggcttctt ctccgggctg atgctcagcc agtacacggc ggacacgctg atcaccgagc
     1681 agcggatcct gtcctcaccg gcctccgtca tgtcgatccc ggcggcggcg gaccaggagg
     1741 acttcgtctc gatggggatg aacaccgcga tcaagaacgg gcagatcctg gacaacgcct
     1801 acgcggtgat cgggatcgag ctcatggcgg ccgcccaggc cctcgacttc cgagacttcc
     1861 atcccggcaa gggggtgcag gtcgcccacg aggtcgtgcg ccgccacgtc gagcacctgg
     1921 accaggaccg tccgctccac gtggaccacg accgggtgaa ggcgctggtg gcgagcggcg
     1981 aggtcctgga ggccgtggag tcggcggtcg gcccgctgag ctgagggaac gatcgggccg
     2041 gtcccgatcc ggtggtgtcg ttcctgctcg gtccgtctcg cggccacgga ggcgaagctc
     2101 cttcacaggt cagggccctt cggcatatcc ttggtggagt tgaatcaccg ttcaaagagg
     2161 a
//
LOCUS       DASQQQ010000002        12135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_192965, whole genome shotgun sequence.
ACCESSION   DASQQQ010000002 DASQQQ010000000
VERSION     DASQQQ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12135
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192965"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VE646_00015"
     CDS             <1..380
                     /locus_tag="VE646_00015"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZD78416.1"
                     /translation="AGHWIPDMVEIVGGVNLLNGKEQPSRQVTWRQIRDVSPEVVVFM
                     PCGYYLEEAEDELAALMANAEFAETPAARERSVFAVDATSYFSRPGPRIVDGLEIMAW
                     AIHPDAFPHPPEGSIMRAGRRSG"
     gene            392..628
                     /locus_tag="VE646_00020"
     CDS             392..628
                     /locus_tag="VE646_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78417.1"
                     /translation="MRGVNTVAPGDTGKRVSLQFELPNGFVSEVVGTLEAYDYPAETY
                     LVRTKDGSLARVPKRGVRFGKLVPTRTTEQEGPG"
     gene            625..1044
                     /locus_tag="VE646_00025"
     CDS             625..1044
                     /locus_tag="VE646_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HZD78418.1"
                     /translation="MTEDLPSAFIRRRLGSEDAHYGGNLVAGAKQLEIVGDLITELAM
                     RYDGDEGLFRAYDSVEFLAPLYAGDWIEAKGRIVRVGRTSRTCEFEVWKVGGARPDIC
                     ESAAEFIEEPILTCRAVGTTVVREEQQRRSHGAGVSS"
     gene            complement(1046..1465)
                     /locus_tag="VE646_00030"
     CDS             complement(1046..1465)
                     /locus_tag="VE646_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZD78419.1"
                     /translation="MAAQIEPLAAPQEDRTSILVVDDEPQVVWMLQFSLEAEGFQTFA
                     ARDGRMALDEVRRRRPKLMLLDIMMPVMDGWSVLEQMRAEFPDDVRPRVIVVSARSSL
                     RDRAKAAELGADAFVAKPFNVDHLLGVLHDLNGDGAR"
     gene            complement(1628..2635)
                     /gene="purM"
                     /locus_tag="VE646_00035"
     CDS             complement(1628..2635)
                     /gene="purM"
                     /locus_tag="VE646_00035"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZD78420.1"
                     /translation="MSEAYRRAGVDLEAASRAVGMIRDLAAGATRPEVIEGVGGFAGL
                     FRLGSGRYLAAATDGVGTKLEIARMAGRLGSVGIDLVAMCADDVVCTGAEPMLFLDYL
                     AVGRLVPEHVAEVVAGVAEGCRRAGCALLGGETAEHPGVMDADRLDLAGFCVGVVGEE
                     ELLGPARVREGDRLVGLASSGLHSNGFSLVRSALLAQGPAVLEGVPQGFDRPLAELLL
                     EPTAVYAPLVRSLAAGRRIHAAAHITGGGLPENAARILPSGLAARLERGAWSVPAVFE
                     LVRRSARISDRDMFATFNMGIGMVLAVPVGSVDEVLDAAAGSGARVIGSVVPGTGVEL
                     V"
     gene            complement(2654..4057)
                     /gene="purF"
                     /locus_tag="VE646_00040"
     CDS             complement(2654..4057)
                     /gene="purF"
                     /locus_tag="VE646_00040"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZD78421.1"
                     /translation="MGTESPKEACGLFGVWAPGEDVARITYFGLFAQQHRGQESAGMA
                     VSDGQHLLVYKELGLVSQVFNEATLATLQGDLGIGHTRYSTTGSTTWENAQPAFKTDG
                     ERSLALGHNGNLVNTSELAERVGRASGSATTDSDLVATLLSRRHRAGLWDAAAEVLPT
                     LEGAFCFVMMDERSVYAARDPHGLRPLSLGRLPNGFCVASETCALDIVGATYVRDVEP
                     GELLRIDDRGLSSRRFTESAGRSLCIFEFVYLARADSRIGGHTVYQVRRELGRRLAAE
                     APADADLVIPVPDTGHAAAQGYSEVSGIPYGEGLMKNRYVGRTFIQPSQSLRERGVKL
                     KLNPIPDAIRGKRLVVIDDSIIRGTTTRQLVQALREAGAAEVHTRITCPPIEWPCFYG
                     IDMSTRQELVAADLSVEQIREYVGADSLGYLSLDGMVAATGSGKAAFCRACFDGEYPI
                     PIPEGAGKFLLEHPAKS"
     gene            complement(4104..6296)
                     /gene="purL"
                     /locus_tag="VE646_00045"
     CDS             complement(4104..6296)
                     /gene="purL"
                     /locus_tag="VE646_00045"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HZD78422.1"
                     /translation="MDQPLHRALGLTDAELDAIRRAIGREPNRNELAMYAVMWSEHCS
                     YKSSKVHLRRLPTQGPRILVGPGQDAGAVDLGEGLAVVFKMESHSHPSAIEPYQGAAT
                     GVGGIVRDIISMGARPIALLDPLRFGPLSDARNRFLLGGVVAGIGGYGNCIGVPTVGG
                     EVRFAPAHAANPTVNVMCVGIAPADRLVTSAREIPAGSLLLLFGAATGRDGIGGVSVL
                     ASQTLEEGAEEGRPSVQIGDPFAGKLLIEASLELVERDLLEGLQDLGGAGLTCAVSES
                     AARARLGADLNLDAVPLREGDLEPFEILTSESQERMLAIVHPDRMAEARSVCDKWGLA
                     SAVVGGLRQGGGLSVTIGGRVVAEVPARSLADEGPEYHRPMEPPAEVAEALEDDPTFA
                     TIPVTAEDALRSVLASPNVASKRWIWEQYDSIVQGRTVLGPGADAAVIRIDGSLRALV
                     LASDGNGRYGALDPYLGAAHAVAEAARNVATVGATPLAVTNCLNFGNPERPEVMWQFA
                     EAIQGMADACRALQTPVTGGNVSFYNESGGSAIWPTPVIGMVGLIEDYRLLVRPGFRS
                     GTIVYLLGETLPELGGSEFAEVVLGTVSGHPPALDLEVEAALQRLLVKAASQDLLVSA
                     HDCSDGGLAVTLAGSALAGDTGFAVSLPGDVPWYVTLFSESASRAVVSVRAERARELE
                     DLAGALQVPFQRLGETGGPRLVVDGRIDLALEEARRISEGTIPDLMQA"
     gene            complement(6307..7017)
                     /gene="purQ"
                     /locus_tag="VE646_00050"
     CDS             complement(6307..7017)
                     /gene="purQ"
                     /locus_tag="VE646_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HZD78423.1"
                     /translation="MTPRPPRVGVVTFPGSLDDRDALRAVAVMGGDPVPLWHAERDLH
                     GVDAVILPGGFSYGDYLRTGAIARFAPVMDEVRSFGARGGPVVGICNGFQILCEAGLL
                     PGALVRNRQLRFVCRWVHVRVETTRSPLSGRVREGEVLRIPVKHGVGRFLAAAGDLRR
                     IEGEGLVVFRYCAPDGTVDDEHDVNGSLNAIAGVRNDRGNVVGLMPHPEHAVDPDLGP
                     AGGQPLFASLLASALAGV"
     gene            complement(7014..7295)
                     /gene="purS"
                     /locus_tag="VE646_00055"
     CDS             complement(7014..7295)
                     /gene="purS"
                     /locus_tag="VE646_00055"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HZD78424.1"
                     /translation="MRFRCSVEVDLKPGLLDPQGKAVEGALPAMGWTNVSNVRVGKHI
                     QLELEAPDAEAALTQVEEMAERLLSNPVIERFAVRSVEELAGLSSEGLL"
     gene            complement(7292..8245)
                     /locus_tag="VE646_00060"
     CDS             complement(7292..8245)
                     /locus_tag="VE646_00060"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HZD78425.1"
                     /translation="MTQGGKLEAQGKVRDIYDAGRDRLLLVATDRISAFDVVLPNPIP
                     DKGRVLTGLSLFWFGRTGDLVANHLISADRRDFPEPFRSEPSLSGRSTLVRKARVIPV
                     ECVARGYLTGSGLKQYRAEGHVCGVPLPPGLEESSRLPEPIFTPTTKASEGHDLPLTF
                     EQTIDLVGKGLAERLRELTIALYERGAQVAAERGIIVADTKFEFGFADGEPILIDEVL
                     TPDSSRFWPADGYAPGRSQPSFDKQFVRDWLDASGWDHEPPPPELPAEVVGKTALKYH
                     EAYERITGEKWDHYRHRVGIMSDHDWERCAVDLERLRGEHA"
     gene            complement(8253..9566)
                     /gene="purB"
                     /locus_tag="VE646_00065"
     CDS             complement(8253..9566)
                     /gene="purB"
                     /locus_tag="VE646_00065"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013668513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HZD78426.1"
                     /translation="MIPRYTSPEMAELWSDRARLGNWLQIEILAVEAWSRLGRIPSED
                     ARAVRERATFTVDRVQELERVTRHDVAAFVQCVGESVGPAGRWVHFGLTSSDVLDTGF
                     ALQLREASDLLLSRLERLLGVVKRRAFEHRQTVMIGRTHGVHAEPTSFGHVLALWAFQ
                     LDRDRDRLRRARDAVAVGKISGVVGTYAQVDPRIEEQVCAHLGLRPAEASSQIVPRDR
                     HAEYLSALALLATSLDLFATELRHLARTEIREVQEPFAQGQKGSSAMPHKRNPVRCER
                     ITGLARVVRGNLQAALEDVVLWHQRDISHSSAERVIFPDSTAAVDFMLAELADVLDGL
                     RVDPDRMRANLEADGGLAFSQAVLLALVDAGCSRDEAYAAVQRAAAAAWDRGADFRRT
                     LSEDPGVAGSLSEARLDVLFDPSRYLRNLDAVFDRLAKVPLTEEG"
     gene            complement(9601..10077)
                     /locus_tag="VE646_00070"
     CDS             complement(9601..10077)
                     /locus_tag="VE646_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HZD78427.1"
                     /translation="MKDAGPAVGVLAASPAELSMMQQATAILEKLEVPHEIRVMSVAR
                     SPDLVDEYARTAFERGLRVIVCTTGISGHLAAGVASRTVLPVIGVPIASPPEMGGSEA
                     LIITVQQPMGVPVAAVGVDAAVNAAVLAAQIVGVTDPELQKRLWKFKDDLSEGLRL"
     gene            complement(10074..11351)
                     /gene="purD"
                     /locus_tag="VE646_00075"
     CDS             complement(10074..11351)
                     /gene="purD"
                     /locus_tag="VE646_00075"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009675240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZD78428.1"
                     /translation="MRLLVVGGGGREHALVRQLAQSPSVDRVFAAPGNPGIAEQATCL
                     PVAAHDLAGIAAAADRESIDLTVVGPEGPLVGGLVDELESRGHRAFGPRRAAARIEGS
                     KAWTKDLCGRYGIPAGRSRAFTEMGPAVEYLDALEPPYVVKADGLAAGKGVVIAESRA
                     DAIAALEASLVERVFGPAGTTVLVEEHLQGPEVSAFALTDGRTVLPLVMAQDFKRVGE
                     GDRGPNTGGMGAYSPVPFVDESTARSIWEQVIERAVRAMSSEGVTYRGVLYAGVMLTE
                     DGPKLLEFNARFGDPETEVILPRLDSDLAELLRAAAEGTLAGRRAEWSARACVTVVLA
                     SGGYPGRPATGLPISGLSGAGEVEGVVVFHAGTAEREGRVVTSGGRVLMVSGLGADLA
                     QARARAYEGCARISFEGMQFRRDIAQRVTGEDR"
     gene            complement(11348..>12135)
                     /locus_tag="VE646_00080"
     CDS             complement(11348..>12135)
                     /locus_tag="VE646_00080"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HZD78429.1"
                     /translation="LREKNLVLTKVYNRLPLQAEGIVEDYLAYAERLRSYVADTGKLI
                     HDALRAGKQVLLEGAQGTMLDLDHGTYPFVTSSNPVAGYALASAGIGPGEVGRVIGIA
                     KAYVTRVGSGPFPTEDTGPDGERLGDVGQEFGATTGRRRRCGWYDAVIMRYAARLNGL
                     TEVFLTKLDVLSGFATVKVCTAYRADGERFEDVPPHQSLFHRAEPEYEELEGWRQDLA
                     GVRRYEDLPSPARAYVERLQELAGVRISTVSVGPAREQSLPVP"
BASE COUNT         1828 a         4593 c         4069 g         1645 t
ORIGIN      
        1 tggccgggca ctggatcccc gacatggtgg agatcgtcgg cggcgtgaac ctgttgaacg
       61 ggaaggagca gccttcgcgc caggtgacct ggcggcagat cagggacgtg tcgccggagg
      121 tcgtcgtgtt catgccgtgc gggtactacc tggaggaggc ggaggatgag ctcgcggcac
      181 tgatggccaa cgccgagttc gccgagacgc cggcggcgcg cgagcgtagc gtgttcgcgg
      241 tcgacgccac ctcctacttc tcgcgtccgg gccctcggat cgtggacggc ttggagatca
      301 tggcctgggc gatccatccc gacgccttcc cccatccccc ggagggatcg atcatgcggg
      361 ccggcaggcg gagcggatag gctgggccct catgagaggc gtgaacaccg tcgcccccgg
      421 tgacaccggc aagcgggtct cgctccagtt cgagctgccc aacgggttcg tgagcgaggt
      481 cgtcgggacg ctggaggcct acgactaccc cgcggagacg tacctggtac gtacgaagga
      541 cgggtcgctg gcccgagtcc ccaagcgcgg ggtccgcttc ggcaaactgg tgcccacacg
      601 gaccaccgaa caggagggac cgggatgacg gaggatctgc cgagcgcgtt catccgacgc
      661 cgcctcggct ccgaggacgc gcactacggc gggaacctcg tggccggagc gaagcagctc
      721 gagatcgtgg gggacctgat caccgagctc gcgatgcggt acgacggcga cgaggggctg
      781 ttccgcgcct acgactcggt ggagttcctg gccccgctct acgccgggga ttggatcgag
      841 gccaagggcc ggatcgtccg cgtgggtcgg acctcacgaa cctgcgagtt cgaggtctgg
      901 aaggtcggcg gcgcccgacc ggacatctgc gagtcggccg ccgagttcat cgaggagccg
      961 atcctcacct gccgcgccgt gggcaccacg gtggtccgcg aggagcagca gcgtcgctcg
     1021 cacggtgcgg gggtgagctc ctagctcacc gggccccgtc gccgttcaga tcgtgcagca
     1081 cgcccaacag atggtccacg ttgaacggct tggccacgaa ggcgtccgca ccgagctcgg
     1141 cggccttggc ccggtcgcgc aggctggacc gggcggatac gacgatcacc cgcgggcgga
     1201 catcatcggg gaactccgcg cgcatctgct ccaggacgct ccagccgtcc atcaccggca
     1261 tcatgatgtc caggagcatc agcttgggtc ggcgccggcg gacctcgtcc aacgccatgc
     1321 ggccgtcgcg ggcggcgaag gtctggaagc cctcggcctc caggctgaac tggagcatcc
     1381 agacgacctg cggctcgtcg tccaccacga ggatgctggt gcgatcctcc tgcggagcgg
     1441 cgagaggctc gatctgcgct gccacggcac tcatatccat cttgggcatc ggcggcgctc
     1501 gggcgggcct ggagtggcca aggataggtc gggcgggtgg ttctgccgga ccggaggtcc
     1561 tagtaccagg gatcggtggg cccgatatag gggggcgggg gaggactagt cacccggcgg
     1621 cagccggtca caccagctcc acgccggtcc cggggaccac cgagccgatc acgcgagccc
     1681 ccgaccccgc ggcggcgtcc agcacctcgt ccaccgagcc gaccggcacg gccagcacca
     1741 tcccgatccc catgttgaag gtggcgaaca tgtcccgatc cgagatccgg gcggatcggc
     1801 ggacgagctc gaagaccgcc gggacggacc aggcgccccg ctccaggcgg gctgcgaggc
     1861 ccgaggggag gatcctggcc gcgttctcgg gcagcccgcc gcccgtgata tgcgccgccg
     1921 cgtggatcct ccttccggct gcgagcgacc ggaccagcgg cgcgtagacg gccgtgggtt
     1981 ccaggagcag ctccgccagg ggccggtcga aaccctgggg caccccctcc agcaccgcgg
     2041 gcccctgggc caggagggcg gagcggacca ggctgaaccc gttcgagtgg agcccgctcg
     2101 atgccagccc caccagacga tcgccctccc gcacccgggc cggaccgagg agctcttcct
     2161 cccccacgac ccccacgcag aatcccgcca ggtccagccg gtcggcatcc atcacccccg
     2221 ggtgctcggc cgtctcgccg cccagaagcg cgcaccccgc ccgccgacag ccctccgcca
     2281 cgccggccac cacctccgcc acgtgctcgg gaacgagtcg tcctacggcc aggtagtcca
     2341 ggaagagcat gggctccgcg cccgtgcaca cgacgtcgtc cgcgcacatc gccaccaggt
     2401 cgatccccac ggagccgagc cgccccgcca tccgggcgat ttccagcttg gtgcccacgc
     2461 cgtcggtggc ggcggccagg taccggcccg agcccaggcg gaacaacccc gcgaacccac
     2521 ccacgccctc gatcacctcg gggcgggtgg cgccggcggc caggtcccgg atcatcccga
     2581 cggccctgct cgccgcctcc agatccaccc cggcccggcg ataggcctcg ctcacgcggt
     2641 gctcaccggc cgctcacgac ttcgccggat gctccagcag gaacttgccg gccccctcgg
     2701 ggatcgggat cgggtactca ccgtcgaagc aggctcggca gaaggccgcc ttccccgagc
     2761 cggtggctgc caccatgccg tccagggaga ggtacccgag ggaatccgcg cccacgtact
     2821 cgcggatctg ctccacggag aggtcggcgg cgaccagctc ctgccgcgtc gacatgtcga
     2881 tcccgtagaa gcacggccac tcgatcggcg ggcaggtgat gcgggtatgg acctcggcgg
     2941 ccccggcctc gcggagtgct tgaaccagct ggcgggtcgt ggtgccccgg atgatcgagt
     3001 cgtcgatcac caccagacgc ttgccgcgga tcgcgtcggg gatcgggttg agcttcagct
     3061 tgaccccccg ctccctgagc gactgcgagg gctggataaa ggtccgaccc acgtagcggt
     3121 tcttcatgag cccctcgccg taggggatgc ccgacacctc gctgtacccc tgggcggccg
     3181 cgtggcccgt gtcggggacc gggatcacca ggtcggcgtc ggccggtgcc tcggcggcca
     3241 gtcgccgacc gagctcccga cggacctggt agacggtgtg gccgccgatc cgggagtcgg
     3301 ctcgggccag gtagacgaac tcgaagatgc acagcgaccg ccccgcgctc tcggtgaacc
     3361 tgcgcgagga tagcccccga tcgtcgatcc gcagcagctc cccgggttcc acgtcgcgca
     3421 cgtaggtggc gcccacgatg tccagtgcgc aggtctcgga ggcgacgcag aagccgttcg
     3481 ggagccggcc cagggagagg gggcgcaggc cgtgggggtc gcgggccgcg tacacgctcc
     3541 gctcgtccat catcacgaag cagaaggcac cctccagggt gggcagcacc tcggctgccg
     3601 catcccacag cccggcccgg tgacggcggg acagcagggt ggcgaccagg tcagagtccg
     3661 tggtggcaga gcctgacgcc cgcccgaccc gctcggcgag ctccgaggtg ttcacgaggt
     3721 tgccgttatg ccccagggcc agggatcgct ccccatccgt cttgaacgcc ggctgcgcgt
     3781 tctcccacgt ggtggagccg gtggtcgagt accgcgtgtg cccgatgccg aggtcaccct
     3841 gcagcgtggc cagcgtcgcc tcgttgaaga cctgggagac gaggccgagc tccttgtaga
     3901 cgagcaggtg ctgcccgtcg gagaccgcca tcccggccga ctcctggccc cggtgctgct
     3961 gagcgaagag accgaagtag gtgatccgcg ccacgtcctc gcccggcgcc cacacgccga
     4021 acaggccgca cgcttccttc ggactctccg ttcccaccgc tgggcggatg gtagcactcg
     4081 ccccccgacc gatggacggg tcttcaggcc tgcatcagat ccgggatcgt cccctcggag
     4141 atgcgtcgag cctcctccag ggcgaggtcg atccgcccat cgacgaccag ccgcgggcca
     4201 cccgtctccc ccagccgctg gaacggcacc tggagggccc cggccaggtc ctccagctca
     4261 cgggcccgct ccgctcggac cgagaccacc gcccgagagg ccgactcgga gaagagggtc
     4321 acgtaccagg gcacgtcccc gggcaggctc accgcgaagc ccgtgtcgcc ggccagggcc
     4381 gaaccggcca aggtcacggc cagcccccca tccgagcagt cgtgcgccga gaccaacagg
     4441 tcctgggatg ccgccttcac cagcagccgc tgcagggccg cctccacttc caggtccagc
     4501 gcgggcgggt gccccgatac ggttcccagc accacctcgg cgaactcgga gcccccgagc
     4561 tccgggaggg tctctcccaa caggtagacg atggtgcccg accggaaccc gggacggacc
     4621 agcaggcggt agtcctcgat cagaccgacc atcccgatca ccggcgtggg ccagatcgcg
     4681 gagccgccgg actcattgta gaaactcacg ttccccccgg tcaccggcgt ctggagcgcg
     4741 cggcaggcat cggccatccc ctggatcgcc tcggcgaact gccacatcac ctccggccgc
     4801 tcggggttcc cgaagttcag gcagttcgtc acggccagcg gcgtggcgcc caccgtggcc
     4861 acgttccggg cggcctcggc caccgcgtgg gccgcgccca ggtagggatc gagcgccccg
     4921 taccgcccgt tcccgtccga ggccaggacc agcgcgcgca gggacccgtc gatcctgatc
     4981 acggccgcgt cggcccccgg cccgagcacc gtcctgccct gcacgatcga gtcgtattgc
     5041 tcccagatcc atcgcttcga ggccacgttc ggggatgcca ggacggagcg cagggcgtcc
     5101 tcggcggtga ccgggatcgt ggcgaaggtg ggatcgtcct ccagcgcctc ggccacctct
     5161 gccggtggct ccatcggccg atggtactcg ggtccctcgt cggccaggga ccgggccggg
     5221 acctcggcca ccacccgtcc cccgatcgtc accgagagcc ccccgccctg ccgcaggccg
     5281 cccaccaccg ccgaggccag cccccacttg tcgcagaccg accgggcctc cgccatccgg
     5341 tcggggtgga cgatggccag catccgctcc tgcgattcgc tggtcaggat ctcgaacggc
     5401 tccagatcgc cctcgcgcag cggaaccgcg tccaggttca gatccgcccc cagccgagcc
     5461 ctggccgccg actcgctgac cgcacaggtc agtccggcgc cgcccagatc ctggagcccc
     5521 tccagcaggt cacgctccac cagctccaac gaggcctcga tcaacagctt cccggcgaac
     5581 gggtcaccga tctggacgct gggccgaccc tcctccgccc cctcctcgag ggtctgcgac
     5641 gccagcaccg ataccccgcc gatcccgtcg cggccggtgg ccgcgccgaa caggagcagg
     5701 agggaaccgg cggggatctc ccgggcggag gtcaccaggc gatccgccgg agcgatgccc
     5761 acgcacatca cgttcaccgt ggggttcgcg gcgtgggccg gggcgaagcg cacctcgcct
     5821 cccacggtgg ggaccccgat gcagttcccg tagccgccga tcccggcgac cacgccgccc
     5881 agcaggaacc gattcctggc gtcgctcagc gggccgaacc ggagcggatc caggagcgcg
     5941 atgggccggg cgcccatcga gatgatgtcg cggacgatgc cgcccacgcc ggttgccgcc
     6001 ccctggtagg gctcgatggc cgaggggtgg gagtgcgact ccatcttgaa gaccacggcc
     6061 aggccctctc ccaggtccac ggcccccgcg tcctgacccg ggccgaccag gatccggggg
     6121 ccctgggtgg gcaggcggcg caggtggacc ttgctcgact tgtacgagca gtgctcggac
     6181 cacatcaccg cgtacatcgc gagctcgttc cggttcggct cccgcccgat cgcgcggcgg
     6241 atcgcgtcca gctcggcgtc ggtcaggccc agcgctcggt gcagcggctg gtccatcgcc
     6301 accgacctag acgcctgcca gcgccgaggc caggagcgac gcgaacagcg gttggccgcc
     6361 cgccggcccg aggtcgggat ccacggcgtg ctccgggtgc ggcatcaggc cgaccacgtt
     6421 cccccgatcg tttcgcaccc cggcgatcgc gttcaggctg ccgttgacat cgtgctcgtc
     6481 gtccaccgtg ccgtccggcg cgcagtagcg gaacaccacc agcccctcgc cctcgatccg
     6541 gcggaggtcg ccggcggccg ccaggaaccg ccccacgccg tgcttgacgg ggatccgcag
     6601 cacctccccc tcgcgcaccc gtcccgagag cggcgagcgt gtggtctcca cccgaacgtg
     6661 gacccaccgg cacacgaacc gcagctgtcg gttgcgcacc agggcgccag ggaggaggcc
     6721 ggcctcgcag aggatctgga acccgttgca gatccccacc accggtccac cgcgcgcccc
     6781 gaacgacctc acctcgtcca tcaccggcgc gaaccgcgcg atggccccgg tccgcagata
     6841 gtcgccgtag gagaaccccc ccggcaggat caccgcgtcc acaccatgga ggtcgcgctc
     6901 cgcgtgccag agcggcaccg ggtccccgcc catgacggcg accgctcgga gggcatcgcg
     6961 gtcgtccagc gatccgggga aggtcaccac ccccaccctg ggcgggcgtg gcgtcacaga
     7021 agtccctccg agctcagccc ggcgagctcc tcgacgcttc ggaccgcgaa gcgctcgatc
     7081 accgggttcg agaggagccg ctcggccatc tcctcgacct gggtcagagc agcctcggca
     7141 tccggcgcct cgagctcgag ctggatgtgc ttgcccacgc ggacgttcga gacgttcgtc
     7201 catcccatcg ccgggagcgc cccctccacg gccttgccct gcgggtcgag caatcccggc
     7261 ttgaggtcca cctccacgct gcaacggaac ctcatgcgtg ctccccacgg agccgctcca
     7321 ggtccacggc gcaccgttcc cagtcgtgat cggacatgat ccccacccgg tgccggtagt
     7381 gatcccactt ctccccggtg atccgctcgt acgcctcgtg gtacttgagg gcggtcttgc
     7441 ccaccacctc cgccggcaac tcggggggcg gtggctcatg gtcccatccg ctggcatcga
     7501 gccagtcgcg gacgaactgc ttgtcgaagc tcggttggga ccggcctggg gcgtacccgt
     7561 cggccggcca gaagcgggag gaatcgggcg tgagcacctc gtcgatcagg atcggctcgc
     7621 cgtccgcgaa cccgaactcg aacttcgtgt cggccacgat gatgccacgc tcggcggcga
     7681 cctgcgcccc ccgttcgtac agggcgatcg tgagctcgcg caggcgctcc gccaggccct
     7741 tgcccaccag gtcgatcgtc tgctcgaagg tcaagggaag gtcgtgtccc tccgaggcct
     7801 tcgtggtggg ggtgaagatg ggctcgggga gcctcgagga ctcctccagg cccgggggca
     7861 gcggcacgcc gcagacgtgt ccctcggccc ggtactgctt cagccccgaa cccgtcaggt
     7921 aaccgcgggc cacgcactcc acggggatca cccgcgcctt gcgcaccagg gtcgagcgcc
     7981 ccgacaggga cggttcgctg cggaagggct cggggaagtc ccgacgatcg gcgctgatca
     8041 ggtggttggc caccaggtcg cccgtccggc cgaaccagaa cagcgacagc ccggtgagga
     8101 cgcgcccctt atcggggatc gggttcggga gcaccacgtc gaaggccgag atgcggtcgg
     8161 tggccaccag cagcagccgg tcccgacccg cgtcgtagat gtcccggacc ttgccctgcg
     8221 cctcgagctt cccaccctgg gtcatcgctt cctcacccct cctccgtcag cggaaccttg
     8281 gccagtcggt cgaacacggc gtccaggttc cgcaggtacc tggacgggtc gaacagaacg
     8341 tcgagtctcg cctccgagag gctcccggcg acccccggat cctcggagag cgtccggcgg
     8401 aagtcggcgc cccggtccca ggcggccgcc gcggcccgtt gcaccgccgc gtaggcctcg
     8461 tcacgggagc agccggcgtc caccagcgcc agcagcacag cctgggagaa agccagcccc
     8521 ccgtccgcct ccaggttggc tcgcatccgg tccgggtcca cgcgcagccc gtcgagcacg
     8581 tcggcgagct cggccagcat gaagtccacg gcggccgtgg agtccgggaa gatcacgcgc
     8641 tcggccgacg agtgggagat gtcgcgctga tgccagagga ccacgtcctc cagtgccgcc
     8701 tgcaggttcc cccgtaccac tcgggccagc ccggtgatcc gctcgcaccg caccgggttg
     8761 cgcttgtgcg gcatggcgct ggagcccttc tggccctgcg cgaacggctc ctgcacctcc
     8821 cggatctcgg tccgggccag gtgccggagc tccgtggcga acaggtcgag cgacgtggcc
     8881 agcagggcga gcgcggacag gtactcggcg tgacggtccc gagggacgat ctggctcgag
     8941 gcctccgccg gccggagccc gaggtgggca cagacctgct cctcgatgcg gggatcgacc
     9001 tgcgcgtagg tccccaccac cccggagatc ttgcccaccg ccacggcgtc gcgagcccgt
     9061 cggagccggt cgcggtcgcg atcgagctgg aaggcccaga gggccagcac gtgaccgaag
     9121 ctggtcggct cggcatggac cccgtgcgtg cgcccgatca tcacggtctg gcgatgctcg
     9181 aacgcccggc gcttcaccac gccgagcaac cgctccagcc gggacaggag caggtccgag
     9241 gcctcccgca actgcagggc gaaccccgtg tccagcacgt ccgatgaggt cagcccgaag
     9301 tgcacccacc ggccggccgg gccgacggac tcgcccacgc actgcacgaa cgcagcgacg
     9361 tcgtgccggg tgacccgctc cagctcctgg acccggtcca cggtgaaggt tgcccgctcc
     9421 cgaacggccc gggcatcctc cgaggggatc cgacccagcc gggaccaggc ctccacggcg
     9481 aggatctcga tctggagcca gttgcccagg cgcgctcgat cggaccacag ctccgccatc
     9541 tcgggcgacg tgtagcgggg gatcatgcgc cggctcccgg tggcagcccg cggagggggc
     9601 tcacaggcgg agcccctccg acagatcgtc cttgaacttc cacaaccgct tctgcagctc
     9661 ggggtcggtg acccccacga tctgagccgc cagcacggcg gcgttgaccg cggcgtccac
     9721 gccgaccgcg gccaccggga cacccatggg ctgctgcacc gtgatgatca gcgcctcgga
     9781 gccgcccatc tccggcggcg aggcgatggg tacgccgatc accggcaaca ccgtgcgaga
     9841 cgccaccccg gcggccaggt gccccgagat gccggtggtg cagacgatca ccctcaggcc
     9901 tcgctcgaac gccgtccgcg cgtactcgtc caccaggtcg gggctccggg ccacggacat
     9961 cacccggatc tcgtgcggca cctcgagctt ctcgaggatc gccgtagcct gttgcatcat
    10021 cgacagctcg gctggcgagg ccgccagcac ccccacggcc ggcccggcat ccttcatcga
    10081 tcctcccccg tcacccgctg cgcgatgtct cgccggaact gcataccctc gaaggagatc
    10141 cgtgcgcatc cctcgtaggc gcgggcgcgg gcctgggcca gatcggcgcc gagaccgctc
    10201 accatcagga cccgcccacc cgaggtgacc actctacctt ctcgctccgc ggtgccggcg
    10261 tggaagacca ccacaccctc gacctcgccc gcgccggaca gccccgagat cggcaggccg
    10321 gtcgcgggcc gccctggata gcccccggat gcgaggacca cggtcacgca tgcccgggca
    10381 gaccactcgg cacgtcgacc ggccagggtt ccctcggcgg cggcccggag cagctcggcc
    10441 aggtccgagt ccaggcgggg gaggatgacc tccgtctccg gatcgccgaa ccgcgcgttg
    10501 aactcgagca gcttcggtcc gtcctccgtg agcatgacac ccgcatacag gaccccccga
    10561 taggtcaccc cttccgaact catggcccgt acggcgcgct cgatcacctg ctcccagatg
    10621 gagcgggccg tggactcgtc cacgaacggc acgggcgagt aggcccccat gccgcccgtg
    10681 ttcgggcccc ggtcgccctc tcccacccgc ttgaagtcct gcgccatcac cagcgggagc
    10741 accgttcgcc cgtccgtgag cgcgaaggcg gagacctccg gcccctgcag gtgctcctcg
    10801 accagcacgg tggtcccggc cggcccgaag acccgttcca ccagcgaggc ctccagggcg
    10861 gcgatcgcgt cggcccggct ctccgcgatc accacgccct tgccggcggc cagcccatcc
    10921 gccttcacca cgtagggagg ctcgagcgcg tccagatact cgaccgccgg tcccatctcg
    10981 gtgaaggctc gggagcggcc cgccgggatc ccgtaccgcc cgcacaggtc cttggtccag
    11041 gccttcgagc cctcgatccg tgcggccgcc cgccggggac cgaacgcccg gtgcccccgt
    11101 gactcgagct cgtccaccag gccccccacc agcggcccct ccgggcccac caccgtgagg
    11161 tcgatcgact cccggtccgc ggcggcggcg atgcccgcca ggtcgtgggc ggccaccggc
    11221 aggcacgtgg cctgctcggc gatcccgggg ttgcccgggg cggcgaagac ccggtccacc
    11281 gatgggctct gggccagctg ccggaccagg gcgtgctcac gccctccgcc gccgacgacc
    11341 aagagcctca tggcaccggc aggctctgct cgcgggccgg gcccaccgag accgtcgaga
    11401 tccggacccc ggccagctcc tggagccgct ccacgtaggc ccgcgccggc gagggcaggt
    11461 cctcgtaccg gcggaccccg gcgaggtcct gccgccaccc ctccagctcc tcgtactcgg
    11521 gctcggcccg gtggaacagg gactggtgcg gcgggacatc ctcgaaccgc tcaccatcgg
    11581 ctcggtaggc ggtgcagacc ttcaccgtgg cgaagcccga gagcacgtcg agcttggtga
    11641 ggaacacctc ggtcagcccg ttcagccgtg ccgcgtaccg catgatcacg gcgtcgtacc
    11701 atccgcaccg acggcgccgg ccggtggttg ccccgaactc ctgacccacg tcccccagcc
    11761 gctcgccgtc cggtcccgtg tcctcggtgg ggaaggggcc cgagcccacc cgcgtcacgt
    11821 acgccttcgc gatgccgatc acccggccga cctcacccgg gccgatcccg gcggaggcca
    11881 gcgcgtaccc ggccaccggg ttcgatgagg tcacgaaggg ataggtcccg tgatccaggt
    11941 ccagcatggt gccctgcgct ccctccagca ggacctgctt gccggcccgg agggcatcgt
    12001 ggatcagctt gccggtgtcg gccacgtacg accggaggcg ctcggcgtag gccaggtagt
    12061 cctccacgat cccctccgcc tgaaggggca ggcggttgta gaccttcgtc aggaccaggt
    12121 tcttctcacg gagga
//
LOCUS       DASQQQ010000003        20746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_110284, whole genome shotgun sequence.
ACCESSION   DASQQQ010000003 DASQQQ010000000
VERSION     DASQQQ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 20746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20746
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110284"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..768
                     /locus_tag="VE646_00085"
     CDS             148..768
                     /locus_tag="VE646_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HZD78430.1"
                     /translation="MSLLVPTVIEESARGERAFDIYSRLLRERIVFLGSPIDDGVANL
                     VVAQLLFLDSVEAGKDIALYVNSPGGATTALFAIYDAMGSLASDVATYGVGQAASAAA
                     VILAAGTAGKRHALANARVLLHQPHGGVEGQSADLEIHAREAIRQRRRMEEILAEHTG
                     QPVERIHEDLDRDFILDAHEARSYGLVDQVIEPRRLARVAELSRSV"
     gene            complement(788..1078)
                     /locus_tag="VE646_00090"
     CDS             complement(788..1078)
                     /locus_tag="VE646_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZD78431.1"
                     /translation="MTRTRVRRRRIAAVALAAGAVSVLAGPLSRAFPAPRAVPVAEHR
                     YVIRSGDTLWSIARSIAPASDPRPMVDAIAEANQVDPGALIPGRTLVIPSSA"
     gene            1210..1824
                     /gene="lexA"
                     /locus_tag="VE646_00095"
     CDS             1210..1824
                     /gene="lexA"
                     /locus_tag="VE646_00095"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZD78432.1"
                     /translation="MDDVTPRQRRILEFIQRTVRERGYPPTVREIGEAVGLTSSSSVH
                     GQLANLQRKGLLLRDPTKPRAVSLVEPEPARPEPAVVPLIGRIAAGVPVLADQNVTDH
                     LTVPMGFVDGAEHFALRVSGDSMIEAGIFDGDIVIVRRQDTVDDGDVVAAVLPGPAED
                     EATVKRLRRKGSKIMLVPENRSMSPFEMTEGNIAGKVVAVLRKL"
     gene            1858..2841
                     /locus_tag="VE646_00100"
     CDS             1858..2841
                     /locus_tag="VE646_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HZD78433.1"
                     /translation="MGDTALERAIRAQPDELERLASQRIPHDLTERLLQARRLWLVGT
                     GTSFHAAELGATMLQQAGRGAHALPSMRFVNWAPPVHPQDGVIVISHNAGRETAYAYA
                     AYAMAREAGLRVVAITRREGGLPNSLETVDQERSQTYTVSYTAVLLLLARLAHELGAE
                     DLSPDAVAAVPAAVRDALAAPGTDAIPTPRRLLVLAGEGPAAVTAREGALKLREGARL
                     PAEGYEVEYLLHGSAVPIGPDDHLIVLGSPDTHGLVRAVGAAAAAEGVPVTSLVETAS
                     LPPLLAQIPLTVRLQLLALRQAEERGMDPDEVITGAWADEELWRIGTPERG"
     gene            complement(2870..4201)
                     /gene="hflX"
                     /locus_tag="VE646_00105"
     CDS             complement(2870..4201)
                     /gene="hflX"
                     /locus_tag="VE646_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014694097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HZD78434.1"
                     /translation="MSQDGRRRGRRSVMESPLSATWTPRVREKAVLVGVGHGMEEADL
                     DELAALADSAGAEPVARVVQTRSDPDPATFVGRGKIDEIHRAVHRTGADAVILDDELS
                     AGQLRNLEDRLGVKVIDRTALILDIFALHARSREGKAQVELAQLNYLLPRLRGWGEAM
                     SRLGGGIGTRGPGETKLEVDRQHIKRRITKLRRDIKDLSRTRDVKRARRERSGVPQIA
                     IAGYTNAGKSTLMNALTEASVLVADQVFATLDPTTRRLQLPNGRLAVLSDTVGFVGKL
                     PHDLVEAFRSTLEEVTRADLILHVADASSPDVVEQVDAVRTVLGEIGAGTIPDVLALN
                     KVDLLDDVARERVTRRFPRAVLVSAITGRGQQGLVAAVAEAIPRPPVEVTLLVPFGRE
                     DVTARLHREGEVLTEEPRDEGTLVRARVERRDVPRVAELMVDPSGPHTRAG"
     gene            complement(4201..5355)
                     /locus_tag="VE646_00110"
     CDS             complement(4201..5355)
                     /locus_tag="VE646_00110"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HZD78435.1"
                     /translation="MRIARRIEALPPYLFAALDEKLAAKRAEGVEVISLGVGDPDQPT
                     PEHIVAAMQDAIRDPATHRYPSYYGTIEYRTAVADWYGRRFGVELDPATEVISLIGSK
                     EGVAHLAFAFVDPGDEALVSDPGYPVYATSTQLAGGTPTSLPLVAEHGFLPDLEAVPV
                     SGRTKLCWINFPGNPTGAVADLGFFQRAVAWARDHDVLLAHDAAYSEITFDGFVAPSL
                     LQVPGAKDVAIEFNSLSKFNNMTGWRAGFAVGSARAISALGVVKTNIDSGLFTAIQRA
                     GVAALSGPQEHVEGLRATYQRRRDMVISALNGLGWSLKPPLGSIYVWLPVPEGQTSVG
                     FADLLLDRAGVFVAPGSGYGPHGEGYVRIALTVSEDRLAEAMERLGEALG"
     gene            complement(5352..6221)
                     /gene="dapF"
                     /locus_tag="VE646_00115"
     CDS             complement(5352..6221)
                     /gene="dapF"
                     /locus_tag="VE646_00115"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HZD78436.1"
                     /translation="MEAELRIWKYQGTGNDFVLLEDLEDRRPLSADLVRALCHRRFGV
                     GADGVIRVTRGAQGRLFMDHRNADGSVAEMCGNGIRCLAAFAHERGLVQGTELEVETR
                     AGVKHLTLGMAGDDVGTVTVGMGPARLSRSQIPMTGPPRDTFLTQPVEVDGLTWKGSA
                     LSMGNPHLVLFVEQDPEGVDVPRIGPRLERREWFPQGANVEFVVVQDGGLKVRVWERG
                     VGETMACGTGACAALVAANEAGLVPARAEVRFPGGTLVVERGPEDVWLTGPAERVFEA
                     VLDRGWRGGRGVA"
     gene            complement(6211..7134)
                     /gene="miaA"
                     /locus_tag="VE646_00120"
     CDS             complement(6211..7134)
                     /gene="miaA"
                     /locus_tag="VE646_00120"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZD78437.1"
                     /translation="MARPALLALVGPTASGKTEAGVVLAERLGAEIVSVDSMLVYRGM
                     DVGTDKPDRPLRARVPHHLLDLVEPNQAFSVAQYQRLAHRVLAHIRDRGRPALLVGGS
                     GLYFRAVVDELEFPATDPGTRRDLQAEATALGAEGLHARLALVDPAAAERIEPGNVRR
                     TVRALEVAAVTGRPFSSYAEGWDRYPTERVRAAGIELARPALEARIRARVEAMVAAGL
                     LEEVRGLLDRGLFGWLTASQAIGYAEFARHVRGELSLEQAVEGTVGRTRELARRQMVW
                     FRRDPRIRWFRAGAEGATALGAEIEGYLRGG"
     gene            complement(7200..8507)
                     /gene="miaB"
                     /locus_tag="VE646_00125"
                     /pseudo
     CDS             complement(7200..8507)
                     /gene="miaB"
                     /locus_tag="VE646_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
     gene            complement(8518..10050)
                     /gene="rny"
                     /locus_tag="VE646_00130"
     CDS             complement(8518..10050)
                     /gene="rny"
                     /locus_tag="VE646_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HZD78438.1"
                     /translation="MEILIAGLGAAVGGIAFGYLMRQAIGQSRAETAESKAQKMVLEA
                     EREAEKVVREALVEVKDEIGGMRREAEQDIRTRREEVKRQEQRLAQKEENLERKVGDL
                     DRRVADLDDRDAKLQKVRQQLEQAAEHHRAQLESVARMTTQEAKDALIGQVVEEAKRD
                     AMATVREIEQRAREEGEERARKIVTIAIQRVASEQTSESTVSVFSLPSEDMKGRIIGR
                     EGRNIRAFEATTGVNLIIDDTPEAVVLSCFDPVRRETARLTLEKLVSDGRIHPARIEE
                     TYERSQKDVDERIRRAGEEAVLEAGVTDMHPDLVKLLGRLQFRTSYGQNVLRHLVESS
                     HIAAAIAAELGIDPRIPRRGALLHDIGKAVTHEVEGSHAIIGAEIARRLKESPEVVHC
                     IESHHGEVEQRTVEAVIAQIADGISGGRPGARRESLETYVKRLERLEEICTSYPGVEK
                     VYAMQAGREVRVMVKPAEVDDLAAQVLARDMAKQVEEELQYPGQIKITVVRESRSVEY
                     AR"
     gene            complement(10313..10816)
                     /locus_tag="VE646_00135"
     CDS             complement(10313..10816)
                     /locus_tag="VE646_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HZD78439.1"
                     /translation="MTGPRGSRQPKHRRSCHDRALGLLAVRPRSRRELELRLLRAGFD
                     RDEVAQELERLSAVGLVDDRRFAESFAEHAVGTKLAGRRAVFAALSARGVDRATIERT
                     LAQVPGGEADRAVELARLRATRLTGLPPEVAARRLVGFLARRGYEAGVARRAAAAALG
                     LEGDGSG"
     gene            complement(10825..11919)
                     /gene="recA"
                     /locus_tag="VE646_00140"
     CDS             complement(10825..11919)
                     /gene="recA"
                     /locus_tag="VE646_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016910065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HZD78440.1"
                     /translation="MDRDKMLDVALTQIEKQYGKGAVMRLGEHPMGHGISVIPTGSLA
                     LDLALGVGGIPRGRIVEVFGPEGSGKTTVCLHIIAEAQKRGGIAAFIDAEHALDPTYA
                     RNLGVDIDALLVSQPDSGEQALEIADMLVRSGALDLVVVDSVAALVPRAEIEGEMGDS
                     HVGLQARLMSQAMRKLNGSLSRFETTAIFINQLREKIGVMFGNPETTPGGRALKFYSS
                     VRLDVRKIENLKDATEVVGSRTRVKVVKNKVAPPFRQCEFDIMYGTGISREGSLLDVG
                     VDMELVKKSGAWFTYEGDQLGQGRENARQFLREHADIAAEIERRVREAVGLTSFGEEG
                     EEPVVIEDGGEPATTKGGRKEREATGAPAG"
     gene            complement(12118..12699)
                     /gene="thpR"
                     /locus_tag="VE646_00145"
     CDS             complement(12118..12699)
                     /gene="thpR"
                     /locus_tag="VE646_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HZD78441.1"
                     /translation="MGRDRASRPEAKPLRLFVAVDVPAEVRAAVAEAVAPIREGHPRA
                     RWVPLENQHVTVKFLGRTWPRLTDWVPERVREVAAEVAPFTTRVAGVGAFPSPRRARV
                     LWAGLEDPDGRLAVMAGALDRAMSAEFEPEKRAFTPHLTVARFQPPVAVEEIGAGAEM
                     GEPFPVDRLILYRSHLRRPAPVYEALRVFPLAG"
     gene            complement(12699..13979)
                     /locus_tag="VE646_00150"
     CDS             complement(12699..13979)
                     /locus_tag="VE646_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HZD78442.1"
                     /translation="MSETPIVRAEVIGVGTELLLGQIANTNARWISERLAGVGVDVLH
                     HQAVGDNPGRIVQAIRLALSRSEVVIVTGGLGPTQDDITREAVASAFGLALIRHPEIE
                     ERLRARFERLGRRMPRSNLVQADVPQGARTIVPRLGSAPGLVVEAGGRRLYALPGVPA
                     EMREMMEGTVLPELGRLVGGGAIVSRILRCTGIAESRVAEILDDLFRRSANPTVAYLA
                     GGGEVKVRLTAKAPSAEKAEALLAPLVREAAERIGDFVFTTVDESLEEAVGRLVISSG
                     RTLSCAESLTGGELGARITSVPGASAYFAGSAVCYTAQAKRTILGVSGATLEGPGMVS
                     RPCAREMAVGARRLYGTDLALALTGAAGPEAHAGAEPGAVWVALEAEGVSHQRGLHAP
                     GDRAMVRRWAEQAALDLVRRHLEGRPLPEADRVV"
     gene            complement(13976..14722)
                     /locus_tag="VE646_00155"
     CDS             complement(13976..14722)
                     /locus_tag="VE646_00155"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZD78443.1"
                     /translation="MAPDPEGGVQGTSAGVGSTLRRARTARGVSIPEASRDTRIRPEF
                     LQALEAEEFDRLLGEVHARGCLRSYATYLGLQGDELVGRYARQAPQAAPAPAVAPPPV
                     RPAVGGRRRRDNTRLFVMLAALLLILAAAFGILSSRSSAPPPAPMPSEAPLGAGSIQG
                     LMLVVLARQEVEVTVTVDQGEPESFTLRPDESRSFEAQTAITVLLEHGASANVQVNGV
                     DEGYPGTERHPWEHTYRADQVPPPSPSPLG"
     gene            14761..15285
                     /locus_tag="VE646_00160"
     CDS             14761..15285
                     /locus_tag="VE646_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HZD78444.1"
                     /translation="MAPDLVTVVVEIPKGSRNKYEMDHETGAIVLDRMLFTSMQYPAD
                     YGFIEHTLALDGDPLDALVFVGEPTFPGCHILVRPIGLFRMHDEKGPDEKILCVPIKD
                     PLWSLINELSDVNPNLLNEIEHFFAVYKDLEGHKVGTEGFGEREEALGVIEGAFQRAR
                     EGADRAASDLGPRH"
     gene            15357..15773
                     /locus_tag="VE646_00165"
     CDS             15357..15773
                     /locus_tag="VE646_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78445.1"
                     /translation="MQEDRGGTMDELASDRWGESSPLALVTESVGVPALRAEGAVRYI
                     VVSGDRDTGAWGVVGALWNSLDGNRGGFLVNPNALYTGSEMVKNYRSALRRGWSHEEI
                     YRYWQERAGRVDAGVKVERQQYSETLWRLHRTIQDQ"
     gene            complement(15798..17996)
                     /locus_tag="VE646_00170"
     CDS             complement(15798..17996)
                     /locus_tag="VE646_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HZD78446.1"
                     /translation="MAKAKRRSTRRPPSTRRRRQRTTVRQHLAPWARDALGIGLVVFA
                     LISVLALWFDAAGVVGRGISVLAHGALGIAAVTFPVLALYWGVLLLRSAAQGERVRMF
                     IGFAIALAGVLGLVSLARGDPPPLAGYERLKDAAGVLGAALAWPVGWAVSQVGSAIVC
                     AALAVLGLLIFSGTPLSAAWTRVRDFFTAADVEREEPSPEPAPPRERRRRKRLDTQEE
                     LPQVVDLREPEPEPEPEPVLHVPHPAPTGGAYKLPPLDLLRLAPTSSGSNLDEEHTVE
                     ALERTFHTFGVAARVPAAHRGPTVTLYEVEVEAGTKVNKVLSLADDIAYALATPDVRI
                     IAPIPGRSAIGVEVPNRVRDFVMLGDILRSKAAKEERLPLSVALGKDVHGRAQLVDLT
                     KMPHLLIAGATGAGKSSLINSFITSILMRTTPDQVKLVLVDPKRVELAHFADLPHLLS
                     PVIVHPKRAAEALGWVVREMEERYEALAMVGVRDIDGYREGLTEGTLRIPPGQEDRFG
                     ELPYILVVIDELADLMLVAPRDVEGSIVRIAQMARAVGIHLVVATQRPSVDVVTGLIK
                     ANIPSRIALMTSSQADSRVILDMNGAEKLVGHGDMLFAPSNASKPSRLQAAWVTEKEI
                     EAVSQWIRTQRGPEYSATVEGLGKPPVEGEEDLGGDDLLEQAAELVIRSQLGSTSMLQ
                     RKLKVGFARAGRLMDLMEERGIVGPSQGSKARDVLITWEEWEDSRAARST"
     gene            complement(17997..19697)
                     /locus_tag="VE646_00175"
     CDS             complement(17997..19697)
                     /locus_tag="VE646_00175"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HZD78447.1"
                     /translation="MPASSRAVPVPATGDGPARVVFLGGVGEVGRNMACVELAGRMLV
                     VDVGLSFPHAEMPGIDLVLPDFEYVVEHLDRLEAIVLTHGHEDHIGAMPYLLREVERA
                     VPVYGTPFTLALLEGKLEEHGVRDRAEFRQVVPGEATVAGPFAMRFMRVTHSIPDGMA
                     VAIDTPFGTILHTGDFKIDQTPLDGRPTDLHALAEEAGHGVHLLLSDSTNAEDAGYTK
                     SERSVGPVLREIVARAPQRVVVACFSSHIHRIQQVVNAARANERVVAFLGRSMHQSVD
                     AARRLGLLTVPDADVILIEQVEEMDPSRVVVICTGSQGEPLSALSLMAAREHKWVKLR
                     EGDTVVLSSSLIPGNEPAIHRVVDGLYRTGADVYHLPASPIHASGHAAAEELRLMLSL
                     VRPRWFIPIHGERRHLAHHAKLATEVGVPPDHVLVCEDGDVVEVGERVSIVDRIPAGM
                     TFVDGLGIGDVGEVVLRDRRKLSGEGVVVVVVAVDAHHGEIVAGPDIVNRGFVYEDLA
                     GDILEGARERVMLSLKESAASEVVDRSVLEQNIRRTLGRYFYEVTQRKPVILPVIMEV
                     "
     gene            complement(19681..20565)
                     /gene="dapA"
                     /locus_tag="VE646_00180"
     CDS             complement(19681..20565)
                     /gene="dapA"
                     /locus_tag="VE646_00180"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZD78448.1"
                     /translation="MAGRFGAVATAMVTPFQEDHTLDLEGARELARHLLEHGTDTLVV
                     AGSTGEAPTLSHREKSDLFRAVIEAAEGRGRVVCGTGTYNTRETIELSREAEQLGADG
                     LLLVTPYYNKPPQRGLLEHFTRVASAVDLPIVAYNIPGRTATRIEHDTLLEMTKVPNI
                     VGVKDSTGDFQAISRLIGEAPADFEVYSGDDWATFGYMCLGAVGVVSVAANIIGERVH
                     QLTDLVSSGDIAAARKVHEELTPLFNTLFLTTNPIPVKTALQLLGLPAGPPRLPLVPA
                     TSEERARIQKALVDAGLL"
BASE COUNT         2906 a         7857 c         7154 g         2829 t
ORIGIN      
        1 caggcggacc cggcgccgca ccgggcgggg atcgggtctc ggctcgtcac cgctcatcgc
       61 gaaaccaccc ggttgcgata tccgcggtgc ggcgatacga tcctttgcaa ccgcacggtt
      121 gcgatcatac cccaccgagg aggagccatg agcctgctgg tccccaccgt gatcgaggag
      181 agcgccaggg gggagcgcgc cttcgacatc tactcccgcc tgctccgcga gcggatcgtc
      241 ttcctgggca gcccgatcga cgacggggtc gccaacctgg tcgtggctca gctgctgttc
      301 ctggactccg tcgaggcggg caaggacatc gccctgtacg tgaacagccc gggcggggcc
      361 accacggcct tgttcgcgat ctacgacgcg atgggctcgc tggcctccga cgtggcgacc
      421 tacggcgtgg ggcaggccgc ctcggcggcg gcggtgatcc tggcggcggg cacggccggc
      481 aaacgccatg cgctggccaa cgcccgcgtg ctcctgcatc agccgcacgg tggcgtcgag
      541 gggcagtcgg ccgatctgga gatccacgcc cgggaggcga tccgccagcg gcgccggatg
      601 gaggagatcc tcgccgagca caccgggcag ccggtggagc ggatccacga ggacctggac
      661 cgggacttca tcctcgacgc gcacgaggcc aggtcctacg gcctggtgga ccaggtgatc
      721 gagccacggc ggctggcccg ggtggccgag ctctcgcgaa gcgtctgagg cgcccccgcc
      781 cccgtcctca cgccgaggac gggatcacca gggtccggcc cgggatcagg gccccggggt
      841 ccacctggtt ggcctcggcg atggcatcca ccatggggcg tggatccgag gccggggcga
      901 tcgacctggc gatggaccac agcgtgtcgc ccgagcggat cacgtaccgg tgctccgcca
      961 cgggcacggc ccgcggcgcc gggaacgccc gtgacagcgg cccggccagc acggacaccg
     1021 cgccggccgc cagggccacc gcggcgatcc gtcgccggcg aacacgtgtt cgagtcatga
     1081 cactatccta ccgaacgcgt gttcggggtc aagaccgaac atatgtttga ttccccctcc
     1141 tgctcgtgct accgtcccga tgctaggagg ggggtctgac acggcccatc cgagcggacg
     1201 ggagcgacca tggacgacgt gacgcccagg caacgcagga tcctggagtt catccagcgc
     1261 acggtccgcg agcgcgggta tccccccacg gtgcgcgaga tcggcgaggc cgtgggcctc
     1321 acctcctcat ccagcgtgca cggccagctc gccaacctgc agcgcaaggg gctgctcctg
     1381 cgcgatccca ccaagccgcg ggccgtgagc ctggtcgaac ccgagcccgc tcgccccgag
     1441 ccggcggtcg tgcccctcat cggccggatc gcggccgggg tcccggtgct ggccgaccag
     1501 aacgtgaccg accacctcac ggtcccgatg ggcttcgtcg acggcgccga gcacttcgcc
     1561 ctgcgcgtga gcggggactc catgatcgag gccgggatct tcgacggtga catcgtgatc
     1621 gtccggcgcc aggacacggt ggacgacggc gacgtggtgg cggccgtgct ccccgggccg
     1681 gccgaggacg aggccacggt gaagcggctc cgtcgcaagg ggtcgaagat catgctggtg
     1741 cccgagaacc gctccatgtc gcccttcgag atgaccgagg ggaacatcgc cggcaaggtg
     1801 gtggcggtcc tgcggaagct ctaggctgcc cgggacaccg gcaccagggg gcgaccgatg
     1861 ggtgataccg cgttggagcg cgccatccgc gcgcaacccg atgagctgga gcgcctggcg
     1921 tcccagcgca tccctcacga tctgaccgag cgcctccttc aggcccgccg cctctggctg
     1981 gtggggaccg gcaccagctt ccacgcggcg gagctcggcg ccaccatgct ccagcaggcg
     2041 ggccgcggcg cgcacgccct gccgtccatg cgcttcgtga actgggcccc gccggtgcac
     2101 ccgcaggacg gcgtgatcgt gatcagccac aacgccggcc gggagaccgc gtacgcctat
     2161 gccgcgtacg ccatggcccg tgaggccggc ctgcgggtgg tggccatcac ccggcgggag
     2221 ggtggcctgc cgaactcact cgagaccgtc gatcaggagc gctcgcagac ctacacggtg
     2281 agctacacgg cggtcctgct gttgctcgcc cggctggcgc acgagctggg cgccgaggac
     2341 ctgagccccg acgccgtggc tgccgtgccg gccgccgtcc gggatgccct ggccgctccc
     2401 gggaccgacg ccatccccac gccacgccgc ctgctggtgc tggccgggga gggcccggcg
     2461 gcggtcaccg cccgcgaggg cgccctgaag ctccgcgagg gcgcccgcct gcccgccgag
     2521 ggctacgagg tggagtacct gctgcacggc tcggcggtcc ccataggccc cgacgaccac
     2581 ctgatcgtcc tcggatcacc cgacacccac gggctggtcc gcgcggtggg ggccgcggcc
     2641 gccgccgagg gggtgccggt gacgtcgctg gtcgagacgg catccctgcc accgctgctg
     2701 gcgcagatcc cgctcacggt gcgcctccag ctgctggcgc tgcgccaagc ggaggagcgt
     2761 ggcatggacc cggacgaggt catcaccggg gcctgggcgg acgaggagct ctggcggatc
     2821 ggcaccccgg aacgaggctg atcgagccgg gaacgttggg tggtcccgct cagccggcgc
     2881 gggtgtgcgg cccggaggga tccaccatca gctcggccac ccgcggcacg tcccgccgct
     2941 cgacccgggc ccggaccagc gtcccctcgt cccgaggctc ctccgtcagc acctcgccct
     3001 cccggtggag ccgggcggtc acgtcctcgc gtccgaaggg caccaggagc gtgacctcca
     3061 ccggcggccg ggggatggcc tcggccaccg ccgcgaccag cccctgctgg ccgcgaccgg
     3121 tgatcgccga caccaggacc gccctcggga accgacgggt cacccgctcc cgggccacgt
     3181 cgtccagcag gtccaccttg ttcagggcca ggacgtcggg gatggtgccc gccccgatct
     3241 cgcccaggac cgtgcggacc gcgtccacct gctccaccac gtcgggcgag gaggcatcgg
     3301 ccacgtgcag gatcaggtcg gcccgggtga cctcctcgag cgtggagcgg aacgcctcca
     3361 ccaggtcgtg gggcagcttg cccacgaagc ccacggtgtc ggagagcacc gccagccgcc
     3421 cgttcgggag ttggaggcgg cgggtggtgg gatcaagcgt ggcgaacacc tggtcggcca
     3481 ccagcacgct cgcctcggtc agggcgttca tcagggtcga cttgccggcg ttcgtgtagc
     3541 cggcgatcgc gatctgcgga acgccggagc gctcccgccg ggcccgcttc acgtcgcggg
     3601 tccgggacag gtccttgatg tcccggcgca gcttggtgat ccgtcgcttg atgtgctggc
     3661 ggtcgacctc gagcttggtc tcgccgggac cgcgggtccc gatgccgccg cccagccgcg
     3721 acatggcctc gccccagccc cgcaggcggg gcagcaggta gttgagctgt gcgagctcca
     3781 cctgggcctt gccctcgcgg ctccgggcgt gcaaggcgaa gatgtccagg atcagcgcgg
     3841 tgcgatcgat caccttcacc cccagccggt cctccaggtt ccggagctgg ccggccgaga
     3901 gctcgtcgtc caggatcacc gcgtcggcgc ccgtgcgatg caccgcccgg tggatctcgt
     3961 cgatcttgcc gcgacccacg aacgtggcgg ggtcggggtc ggagcgggtc tggaccaccc
     4021 gggccaccgg ctcggcgccg gccgagtccg ccagggccgc gagctcgtcc aggtcggcct
     4081 cctccatgcc gtgacccaca cccaccagga cggccttctc ccgcacccgg ggggtccagg
     4141 tggccgagag cggtgactcc atcaccgagc ggcgcccccg gcggcgtcca tcctgcgaca
     4201 tcagcccagc gcctccccca gccgctccat cgcttcggcc agccggtcct ccgagacggt
     4261 cagcgcgatc cgcacgtacc cctccccgtg cgggccgtac ccgctgccgg gggcgacgaa
     4321 caccccggcg cgatccagca gcaggtccgc gaagcccacc gaggtctgcc cctcgggcac
     4381 cgggagccag acgtagatgg agccgagcgg cggcttcagc gaccatccca ggccgttcag
     4441 ggcggagatc accatgtccc gccggcgctg gtaggtggcg cgcaggccct ccacgtgctc
     4501 ctgggggccc gacagggcgg cgacgccggc ccgctggatc gccgtgaaca gccccgagtc
     4561 gatgttcgtc ttcaccacgc ccagcgccga gatggcccgg gcgctgccca cggcgaagcc
     4621 cgcgcgccag ccggtcatgt tgttgaactt cgacagcgag ttgaactcga tcgccacgtc
     4681 cttggccccc ggcacctgga ggagcgaggg cgccacgaag ccgtcgaacg tgatctcgga
     4741 gtacgcggcg tcgtgggcca ggagcacgtc gtggtcccgg gcccaggcca ccgcacgctg
     4801 gaagaagccc aggtccgcca ccgcgccggt ggggttgccg gggaagttga tccagcacag
     4861 cttcgtgcgc ccggagaccg gcaccgcctc caggtcgggg aggaagccgt gctcggccac
     4921 caacggcagg gaggtggggg ttcccccggc cagctgggtg ctggtggcgt acacgggata
     4981 cccggggtcg gagaccaagg cctcgtcccc cggatccacg aacgcgaagg ccaggtgcgc
     5041 caccccctcc ttggagccga tcagcgagat cacctcggtg gcggggtcga gctccacgcc
     5101 gaaccggcgc ccgtaccagt cggccacggc ggtccggtac tcgatcgtcc cgtagtagga
     5161 gggatagcgg tgggtggcgg gatcgcggat cgcgtcctgc atcgcggcca cgatgtgttc
     5221 gggcgtgggc tggtcggggt cgccgacgcc gagcgagatc acctcgaccc cctcggcccg
     5281 cttggccgcg agcttctcgt ccagcgccgc gaacaggtac ggcggcagcg cctcgatccg
     5341 tcgtgcgatc ctcatgccac tcctctcccc cctcgccatc cccggtccag caccgcctcg
     5401 aagacccgct cggcgggtcc cgtgagccac acgtcctccg gcccccgctc caccaccagc
     5461 gtcccgccgg ggaaacggac ctcggccctc gccggcacca ggccggcctc gttcgccgcg
     5521 accagggccg cgcaggcccc cgtgccgcag gccatggtct cccccacgcc gcgctcccag
     5581 acccggacct tcaggccgcc gtcctgcacg accacgaact ccacgttcgc accctgcggg
     5641 aaccactcac gccgctccag acgggggccg atcctcggca cgtccacccc ctcgggatcc
     5701 tgctcgacga acaggaccag gtgggggttg cccatcgaga gggcggagcc cttccaggtc
     5761 agcccgtcca cctccacggg ctgcgtcagg aacgtgtccc ggggcggccc ggtcatgggg
     5821 atctgcgacc ggctcagccg ggcgggaccc atccccacgg tcacggtccc gacgtcgtcc
     5881 ccggccatac ccagcgtgag gtgcttcacg ccggcacggg tctccacctc cagctcggtg
     5941 ccctgcacca gcccgcgctc gtgggcgaag gcggccaggc accggatccc gttgccgcac
     6001 atctcggcca ccgagccgtc ggcgttccgg tggtccatga acaggcggcc ctgcgccccc
     6061 cgggtcacgc ggatcacgcc gtcggccccc acgccgaagc gccgatggca cagggcgcgg
     6121 accaggtcgg ccgacagggg ccggcgatcc tccaggtcct ccagcaggac gaagtcgttg
     6181 ccggtgccct ggtacttcca gatccgaagc tcagcctcca cgcaggtacc cctcgatctc
     6241 ggcgcccagc gccgtcgccc cctccgcccc ggcccggaac caccggatcc gggggtcccg
     6301 gcggaaccac accatctggc ggcgggccag ctcccgcgtc cggccgaccg tcccctccac
     6361 cgcctgctcc agggacagct cgccccggac gtgccgggcg aactcagcgt acccgatggc
     6421 ctgggacgcg gtgagccatc cgaacagccc tcggtccagc aggccgcgga cctcctcgag
     6481 cagcccggcg gccaccatcg cctcgacccg ggcgcggatc cgtgcctcca gggccggacg
     6541 ggccagctcg atccccgccg cccggacccg ttcggtgggg taccggtccc acccctccgc
     6601 gtagctcgag aacggccgcc cggtcaccgc ggcgacctcc agcgcgcgga ccgtgcgccg
     6661 cacgttgccc ggctcgatgc gctcggccgc ggccgggtcc accagggcca ggcgggcgtg
     6721 caggccctcc gctcccagcg ccgtcgcctc ggcctgcagg tcccgccgcg tgcccgggtc
     6781 cgtggccggg aactccagct cgtccaccac cgcccggaag tacaggcccg acccgcccac
     6841 cagcagggcc gggcgtcccc gatcgcggat gtgggccagg acccggtgcg cgagccgctg
     6901 gtactgggcc accgagaacg cctggttcgg ctccaccagg tccagcaggt ggtggggcac
     6961 ccgggcccgg agcgggcggt ccggcttgtc ggtgcccacg tccatcccgc ggtagacgag
     7021 catggaatcc accgagacga tctcggcgcc caggcgttcg gcgagcacca cgccggcctc
     7081 ggtcttgccc gatgcggtgg gccccaccag cgcgaggagc gcgggccggg ccatcagcgg
     7141 accggcgccg gctcccgggc ggggccggtg cgggccggac cggcgggggg agcgaccggc
     7201 tcacccacca ggtggtgggg atgggccgac gcgatgcgca cgtggaggaa ggtccccggc
     7261 ggcagctccc gggccacgtg caccacccgg ttggtgcggg tgcgagcctg cgtgccgccc
     7321 ttgcgcccca ggccctcggt caggatctcg acggtgccgc ccacctgggc ccgcatccgc
     7381 tccagggaga tcgcctcctg cagcgccacc agccgatcga agcgctcctg cacgacctcc
     7441 ttgggcaccc ggtcgggcag atcggcggct cgggtcccgg gccggggcga gtactggaac
     7501 gtgtaggcct gatcgaaccg ggcctcgcgc accgtctcca gcgtctgccg gaagtcctcc
     7561 tcggtctcgc cggggaagcc cacgatcacg tcggtggaga cggcgatccg cgggatcgcc
     7621 tgccggatcc gtcccagcca cgagaggtac cggtcccggc ggtaggagcg ccgcatcgcc
     7681 ctcagcaccc ggtccgagcc cgactgcagc gggaagtgga tgtgctcgca cacgctgggc
     7741 gccgaggcca tggcctggat cacgtccggc gtgaagtcgt gcggatgggg gctggtgaac
     7801 cggatccgcc ggatgccctc cacccggtcc accgcccgga gcaggtcggc gaacaggggg
     7861 cgaggaccgg accccggcac ggacagatcc cgcccgtacg tgttcacgtt ctgcccgagc
     7921 agcgtcacct ccaccacccc ccgccgggcc agggcctgga cctcggccag gacgtcgccg
     7981 atcgcgcgcg agcgctgggg cccccggacc agggggacga tgcagaaggt gcaggcgttg
     8041 tcgcatccca cggcgatcga cacccaggcg tggtgggcca gctcgcggcg ggccggcagc
     8101 gcgctgggga agacctcggt gtactcgcgc acgtccatct gccgaccctc gtcgcccgat
     8161 cggcgcagga gctccaggag ctcaggcagg gcgtgggtcc ccaccaccac gtccacccac
     8221 ggcgcgcgct cctggatccg gccccgatcc ttctgggcca ggcatcccgc caccacgatg
     8281 cgcaggtccg ggttggcctg cttcagggac cggaggtgcc ccaggttgcc gtagagccgg
     8341 ttgtcggcgt tctcccggat cgcgcacgtg ttgaacacca cgacctgcgc gtcggccgcc
     8401 cggtcggtcc gcacgtagcc gtccgactcc agcagccccg ccaagcgctc ggagtcgtgc
     8461 tcgttcatct ggcacccgaa ggtgcggatc aggtaacggc cggccacgcc ctccacgcta
     8521 ccgcgcgtac tccaccgagc ggctctcccg gaccaccgtg atcttgatct gccccgggta
     8581 ctggagctcc tcctccacct gcttggccat gtcccgcgcc aggacctgcg cggccaggtc
     8641 gtcgacctcg gcgggcttga ccatgacgcg gacctcgcgg ccggcctgca tcgcgtagac
     8701 cttctccacg cccgggtagc tggtgcagat ctcctccagc cgctccaggc gcttcacgta
     8761 ggtctccagg ctctcgcgcc gcgctcccgg acgccccccg gagatcccgt ccgcgatctg
     8821 tgcgatgacg gcctccacgg tccgctgctc gacctcgccg tggtgggact cgatgcagtg
     8881 caccacctcc ggggactcct tcaggcgccg ggcgatctcg gccccgatga tggcgtgcga
     8941 gccctccacc tcgtgcgtca cggccttgcc gatgtcgtgc agcagcgccc cgcgccgggg
     9001 gatcctggga tcgatcccga gctcggcggc gatggccgcg gcgatgtgcg aggactccac
     9061 caggtgccgg agcacgttct gaccgtagga ggtccggaac tggagccgcc ccagcagctt
     9121 gacgaggtcc gggtgcatgt cggtgacccc ggcctcgagc acggcctcct cccccgcgcg
     9181 ccggatccgt tcatcgacgt ccttctggga gcgctcgtag gtctcctcga tccgcgccgg
     9241 gtggatccgc ccgtcggaca cgagcttctc caacgtgagc cgggcggtct cgcgccggac
     9301 cgggtcgaag caggacagga ccaccgcctc cggcgtgtcg tcgatgatca ggttcacgcc
     9361 ggtggtggcc tcgaaggcgc ggatgttgcg gccctcgcgg ccgatgatgc gtcccttcat
     9421 gtcctcggag ggcagggaga agaccgacac cgtggactct gaggtctgct cggaggccac
     9481 ccgctggatc gcgatggtca cgatcttgcg ggcccgctcc tcgccctcct cccgggcccg
     9541 ctgctcgatc tcgcggaccg tggccatggc gtcgcgcttg gcctcctcca cgacctgtcc
     9601 gatcagggcg tccttggcct cctgggtggt catccgggcg accgactcga gctgggcgcg
     9661 gtgatgctcg gcggcctgct ccagctgttg gcgcaccttc tgcagcttgg cgtcacggtc
     9721 atccagatcg gccacgcgcc ggtccaggtc ccccaccttg cgctcgaggt tctcctcctt
     9781 ctgggccaag cgctgctcct gacgcttgac ctcttcacga cgggtgcgga tgtcctgctc
     9841 cgcctcccgg cgcatccctc cgatctcgtc cttcacctcg accagcgcct cgcggacgac
     9901 cttctccgcc tcgcgctccg cctcgaggac catcttctgt gccttggact ccgccgtctc
     9961 ggctcgcgac tgcccgatcg cctggcgcat caggtagccg aacgcgatcc cgccgacagc
    10021 ggctccgagc cctgcgatca agatctccat atgggctgcc cctccggtcc gacatgaaag
    10081 aacggccgag caaggccctg tccgggctct gctcggccgt gcgtccgatg cgacgaacta
    10141 gggcgccgtc gacccccgtt gggtccttcg ccctcggtct cgtcgcgatg tcttcatctg
    10201 gtctgtcacg tcgtcaggca cacgtcgaac agctgcatat cccaggcaga acctctcaaa
    10261 cgcacaatgt acggcctccc ggccctccag gcaaggcgcc gagcagcacg gctcagccgg
    10321 agccatcgcc ctccagcccc agggccgccg cggccgcccg ccgcgccacc ccggcctcgt
    10381 agccgcgcct ggccaggaag ccgaccaacc ggcgggcggc gacctcgggg ggcagcccgg
    10441 tgagccgggt ggcacggagc cgcgccagct ccaccgcgcg gtcggcctcc cctcccggca
    10501 cctgcgccag ggtccgctcg atggtggctc ggtccacccc cctggcggac agggccgcga
    10561 acacggcccg tcggccggcc agcttggtcc ccaccgcatg ctcggcgaag ctctccgcga
    10621 accggcgatc gtcgaccagc cccaccgcgc tcagccgctc cagctcctgc gccacctcgt
    10681 cccgatcgaa ccccgcccgg agcagccgga gctcgagctc gcgccgcgac cggggtcgga
    10741 cggcgagcag gccgagcgcg cggtcgtggc aggagcgccg gtgcttcggc tggcgggacc
    10801 cccgggggcc ggtcaagcct tcccctaacc cgccggcgcc ccggtggcct cgcgttcctt
    10861 gcgcccaccc ttggtcgtgg cgggctcgcc accgtcctcg atcaccacgg gctcctcgcc
    10921 ctcctcgccg aacgaggtca ggcccacggc ctcgcggacc ctccgttcga tctcggcggc
    10981 gatgtcggcg tgctcccgca ggaactgccg agcgttctcg cgaccctggc cgagctgatc
    11041 gccctcgtag gtgaaccagg caccgctctt cttgaccagc tccatgtcca cgccgacgtc
    11101 gagcaggctc ccctcgcggg agatgccggt gccgtacatg atgtcgaact cgcactgccg
    11161 gaacggcggc gccaccttgt tcttcaccac cttgacccgg gtgcgggacc cgaccacctc
    11221 ggtggcgtcc ttcaggttct cgatcttgcg cacgtccagg cgcaccgacg agtagaactt
    11281 gagggcccgg ccccccggcg tggtctcggg gttgccgaac atcacgccga tcttctcgcg
    11341 cagctggttg atgaagatgg ccgtggtctc gaagcgagac agcgatccgt tcagcttgcg
    11401 catcgcttgc gacatcagcc gggcctgcag cccgacgtgc gagtcgccca tctcgccctc
    11461 gatctccgcc cgggggacga gcgccgccac cgaatcgacc accaccaggt ccagcgcccc
    11521 cgaccgcacg agcatgtcgg cgatctcgag cgcctgctcg ccggagtcgg gctgggagac
    11581 cagcagcgca tcgatgtcca cgccgaggtt ccgggcgtag gtgggatcga gcgcgtgctc
    11641 cgcgtcgatg aaggcggcga tcccgccgcg cttctgggcc tcggcgatga tgtggaggca
    11701 gaccgtggtc ttgcccgagc cctcggggcc gaagacctcc acgatccgcc cccgggggat
    11761 cccgcccacc cccagcgcga ggtccagcgc cagcgagccc gtggggatca ccgagatccc
    11821 gtggcccatg gggtgctcgc ccagacgcat caccgcgccc ttgccgtact gcttctcgat
    11881 ctgggtgagc gccacgtcga gcatcttgtc ccgatccatc cgcgaagcct cctcgctccc
    11941 cctggcccag cggccgagcg tccggctgag ctcctccccc aactccggcc gcacgctatc
    12001 gcgggggtct gacatgggtc acactcctgt gattacagtc ctggaagacg gtcttggcaa
    12061 cccgcagcct agtcgaacac ctgttcgagg tcaacgacct gacctacggc cccacggtca
    12121 gcccgcgagc gggaagaccc tcagcgcctc gtagaccggg gccggacgac gcaggtggct
    12181 gcggtacagg atcagacggt ccaccgggaa cggctccccc atctcggccc cggcaccgat
    12241 ctcctccacg gccaccgggg gctggaaccg ggccaccgtg aggtgcgggg tgaaggcgcg
    12301 cttctccggc tcgaactccg cggacatggc gcggtccagc gccccggcca tcaccgccag
    12361 tcgtccgtcc ggatcctcca gcccggccca cagcacccgg gcccggcggg ggctggggaa
    12421 ggcccccacc cccgccaccc gcgtggtgaa cggcgccacc tccgccgcca cctccctcac
    12481 ccgttcggga acccagtcgg tcaggcgggg ccaggtgcgc cccaggaact tcaccgtgac
    12541 gtgctggttc tcgaggggca cccagcgcgc ccggggatgg ccctcgcgga tgggcgcgac
    12601 ggcctcggcc accgcggccc ggacctccgc cggcacgtcc accgccacga acaaccgcag
    12661 cggcttggcc tcggggcggg aggcccggtc ccgccccatc acaccacccg gtcggcctcg
    12721 ggcagcggac ggccctccag gtgccggcgg accaggtcga gcgccgcttg ctccgcccac
    12781 cgccgcacca tcgcccgatc gccgggcgcg tgcagcccgc gctggtgcga gaccccctcc
    12841 gcctccagcg ccacccacac cgctccgggc tccgcgccgg cgtgggcctc gggcccggcg
    12901 gcgccggtca gcgccagggc caggtcggtc ccgtacaggc ggcgtgcccc gacggccatc
    12961 tcgcgcgcgc aggggcgact gaccatgccg ggcccctcca gggtggcccc cgatacgccg
    13021 aggatcgtcc gcttcgcctg cgccgtgtag cagacggccg agccggcgaa gtacgcggag
    13081 gcgcccggca ccgaggtgat ccgagcgccg agctcacccc ccgtcagcga ctcggcgcag
    13141 gacagggtcc gtcccgacga gatgaccagc cggcccaccg cctcctccag cgactcgtcc
    13201 acggtggtga agacgaagtc cccgatccgc tcggccgcct cccgcaccag cggagcgagc
    13261 agcgcctcgg ccttttcggc cgagggtgcc ttggccgtca gccgcacctt cacctccccc
    13321 ccgccggcca ggtaggccac ggtggggttg gccgagcgcc ggaacagatc gtccaggatc
    13381 tcggccacgc gcgactccgc gatgccggtg caccgcagga tcctggagac gatcgccccc
    13441 ccgcccacca gccgacccag ctccggcagg acggtgccct ccatcatctc ccgcatctcg
    13501 gccggcacgc cggggagcgc gtacaggcga cggccccccg cctccaccac caggccgggg
    13561 gcgctgccca ggcgcggcac gatggtccgg gcgccctggg ggacgtcggc ctgcaccagg
    13621 ttggaccggg gcatgcgccg gccgagccgt tcgaagcggg cgcgcagccg ctcctcgatc
    13681 tcgggatggc ggatcagcgc cagcccgaag gcggacgcca cggcctcccg ggtgatgtcg
    13741 tcctgggtgg ggcccaggcc gccggtcacg atcaccacct ccgagcgcga cagggccagg
    13801 cggatcgcct ggacgatccg tcctgggttg tcccccaccg cctgatggtg caggacgtcc
    13861 acccccaccc cggccaggcg ctccgagatc caccgggcgt tcgtgttggc gatctgcccc
    13921 agcagcagct cggtccccac gccgatcacc tcggcccgca cgatcggggt ttcgctcacc
    13981 cgagggggga gggcgagggt ggggggacct ggtcggcccg gtacgtatgc tcccagggat
    14041 gccgctccgt gcccgggtag ccctcgtcca cgccgttcac ctgcacgttg gcgctggccc
    14101 cgtgctccag gaggaccgtg atcgcggtct gggcctcgaa ggaccggctc tcgtccgggc
    14161 ggagcgtgaa cgactccggc tccccctggt ccaccgtcac ggtcacctcc acctcctggc
    14221 gggccaggac caccagcatc agtccctgga tggatccggc ccccaggggc gcctccgagg
    14281 gcatcggcgc cgggggaggc gccgaggaac ggctggagag gatcccgaac gccgcggcca
    14341 ggatcagcag cagggcggcg agcatcacga acagccgggt gttgtcccga cgacgccggc
    14401 cgcccacggc ggggcggacc ggcggcgggg cgacggccgg ggccggtgcc gcctgcgggg
    14461 cctgtcgcgc gtaccggccc accagctcgt ccccctgcag gccgaggtac gtggcgtagg
    14521 agcgcaggca gccccgggca tggacctcgc ccagcagccg gtcgaactcc tcggcctcca
    14581 gggcctgcag gaactcgggg cggatccgcg tgtcacggct ggcctcgggg atggagacgc
    14641 cgcgcgccgt ccgggcgcgc cgcaacgtcg atccgacccc cgccgaggtc ccctgcacac
    14701 caccctccgg atccggagcc aacggcgatc ataggatacg ggcccggcta gactgcggtg
    14761 atggcacccg acctggtcac cgtggtggtg gagatcccca agggatcgcg caacaagtac
    14821 gagatggacc acgagaccgg ggccatcgtg ctggaccgca tgctgttcac ctcgatgcag
    14881 tacccggccg actacgggtt catcgagcac accctggccc tggacgggga cccgctcgat
    14941 gccctggtgt tcgtgggcga gcccaccttc cccggctgcc acatcctggt ccggccgatc
    15001 gggctgttcc ggatgcacga cgagaagggg ccggacgaga agatcctgtg cgtgccgatc
    15061 aaggacccgc tctggtcgct gatcaacgag ctctccgacg tgaaccccaa cctgctgaac
    15121 gagatcgagc acttcttcgc cgtgtacaag gacctggagg ggcacaaggt cggcaccgag
    15181 ggcttcggcg agcgggagga ggcgctgggg gtgatcgagg gggccttcca gcgcgcccgg
    15241 gagggcgccg atcgggcggc atcggacctc ggccctcggc actaggtcgg gcggcgcgga
    15301 tggggtcgag acttccgacc ggtgccgtgc ccggccgggg gcggtacggt gggcgcatgc
    15361 aggaggaccg gggaggaacg atggacgagc tcgcctccga tcgctgggga gagagctcgc
    15421 ccctggccct ggtcaccgag tcggtcggcg tcccggccct ccgggccgag ggcgcggtcc
    15481 gctacatcgt ggtctccggc gaccgcgaca ccggggcctg gggcgtggtg ggagcgctct
    15541 ggaactcgct ggacgggaat cgtggtgggt tcctggtcaa ccccaacgcc ctgtacacgg
    15601 gcagcgagat ggtgaagaac taccggagcg ccctgcgacg ggggtggagc cacgaggaga
    15661 tctaccgcta ctggcaggaa cgagccggcc gcgtcgacgc cggcgtgaag gtggagcgcc
    15721 agcagtactc ggagacgctg tggcgcctgc accgcacgat ccaggaccag tgaggacggc
    15781 gccggggacg gcgccgctca ggtgctccga gcggcccggg agtcctccca ctcctcccac
    15841 gtgatcaaga cgtcgcgagc cttggagccc tgcgacggtc ccacgatccc ccgctcctcc
    15901 atcaggtcca tcagccggcc ggcccgggcg aaccccacct tcagcttgcg ctgcagcatg
    15961 gaggtggatc cgagctggga gcggatcacc agctcggcgg cctgctccag caggtcgtcc
    16021 ccgcccaggt cctcctcgcc ctcgaccggg ggcttgccca gcccctccac cgtggccgag
    16081 tactcggggc cgcgctgggt gcggatccac tgggagaccg cctcgatctc cttctcggtg
    16141 acccaggccg cctgcagccg gctgggcttg gacgcgttgg agggcgcgaa cagcatgtcc
    16201 ccgtgcccca cgagcttctc cgccccgttc atgtccaaga tcacgcgcga gtcggcctgg
    16261 ctggaggtca tcagcgcgat ccggctgggg atgttcgcct tgatcaggcc ggtgaccacg
    16321 tccaccgagg gccgctgggt ggccaccacc aggtggatcc ccaccgcccg ggccatctgg
    16381 gcgatgcgga cgatcgagcc ctccacgtcg cgcggcgcca ccagcatcag atcggccagc
    16441 tcgtcgatca ccaccaggat gtagggcagc tcgccgaacc gatcctcctg cccgggcggg
    16501 atccggagcg tgccctccgt caggccctcg cggtagccgt cgatgtcgcg aacgcccacc
    16561 atggccaggg cctcgtagcg ctcctccatc tcgcgcacca cccagcccag ggcctccgcc
    16621 gcgcgcttgg ggtgcacgat caccggcgag agcaggtggg gcaggtcggc gaagtgggcc
    16681 agctccaccc gcttgggatc gaccagcacc agcttgacct gatcgggcgt ggtgcgcatc
    16741 aggatcgacg tgatgaacga gttgatcagg ctggacttgc cggccccggt cgcgccggcg
    16801 atcagcaggt gcggcatctt cgtgaggtca accagctgcg cccggccgtg gacgtccttg
    16861 cccagcgcca ccgagagcgg aagccgctcc tccttggccg ccttggagcg caggatgtcg
    16921 cccagcatca cgaagtcgcg cacccggttc ggcacctcca ccccgatcgc cgagcgaccg
    16981 gggatcgggg cgatgatccg gacgtcgggg gtggccaggg cgtaggcgat gtcgtcggcc
    17041 agcgacagga ccttgttcac cttggtgccg gcttcgacct cgacctcgta cagcgtcacc
    17101 gtgggcccgc gatgggccgc cggcacccgg gccgcgaccc cgaaggtgtg gaaggtgcgc
    17161 tccagggcct ccacggtgtg ctcctcgtcc aggttcgagc ccgacgaggt gggggccagc
    17221 cggagcaggt ccagcggcgg cagcttgtag gcgccgccgg tgggggcggg gtggggcacg
    17281 tgcaggaccg gctcgggctc cggttcgggc tcgggctctc gcaggtcgac cacctggggc
    17341 agctcctcct gcgtgtccag gcgcttgcgc cggcgacgct cgcgcggcgg ggccggctcc
    17401 ggggacggct cctcccgctc cacgtccgcg gcggtgaaga agtcgcggac ccgggtccag
    17461 gccgcggaca gcggggtccc ggagaagatc agcagcccca gcaccgccag ggcggcgcac
    17521 acgatggccg agcccacctg ggacaccgcc caccccaccg gccaggccag cgccgcgccg
    17581 agcaccccgg cggcgtcctt cagccgctcg tagcccgcca gcggcggggg gtccccccgg
    17641 gccagggaca ccagcccgag cacccccgcc agggcgatgg cgaacccgat gaacatgcgc
    17701 acgcgctcgc cctgggccgc gctccgcagc agcaggacgc cccagtacag ggccagcacc
    17761 gggaaggtca ccgcggcgat cccgagggcg ccgtgggcga gcacggagat cccccgcccc
    17821 accacgccgg ccgcgtcgaa ccacagcgcc agcaccgata tcagggcgaa gaccaccagg
    17881 ccgatgccca gggcgtcgcg ggcccaggga gcgaggtgct ggcgcacggt ggtccgctgg
    17941 cgacggcggc gcgtgctcgg cggacggcgc gtggacctcc gcttggcctt ggccatctac
    18001 acctccatga tcaccggcag gatcaccggc ttgcgctggg tgacctcgta gaagtaccgg
    18061 cccagcgtcc gtcggatgtt ctgctccagg acgctccggt cgacgacctc cgaggcggcg
    18121 ctctccttca gggacagcat gacgcgttcc cgggcccctt caaggatgtc cccggccagg
    18181 tcctcgtaga cgaacccgcg gttcacgatg tccggaccgg ccacgatctc gccgtggtgg
    18241 gcgtccaccg ccaccaccac cacgaccacg ccctcacccg agagcttgcg gcgatcgcgg
    18301 agcacgacct cgcccacgtc gccgatcccc aggccgtcca cgaaggtcat cccggcgggg
    18361 atgcggtcca cgatgctcac ccgctccccc acctccacca cgtcgccgtc ctcgcagacc
    18421 aggacgtgat cgggcggcac gccgacctcg gtggccagct tggcgtggtg ggccaggtgg
    18481 cggcgctccc cgtggatcgg gatgaaccag cggggtcgga ccagggacag catcaggcga
    18541 agctcctcgg ccgcggcgtg cccggaggcg tggatcgggg aggcgggcag gtggtagacg
    18601 tcggcgccgg tgcggtacag cccgtccacc acccggtgga tcgccggctc gttcccgggg
    18661 atcaggctcg aggacagcac caccgtgtcg ccctcccgca gcttcaccca cttgtgctcg
    18721 cgggcggcca tcagcgacag ggccgagagc ggctcgccct ggctgccggt acagatcacc
    18781 accacccggg aggggtccat ctcctccacc tgctcgatga ggatcacgtc ggcgtccggg
    18841 acggtcagca gccccaggcg ccgggcggcg tcgacgctct ggtgcatcga gcgacccagg
    18901 aacgccacca cccgctcgtt ggcccgggcc gcgttcacca cctgctggat ccggtggatg
    18961 tggctggaga agcaggccac cactacccgt tggggggccc gagccacgat ctcccggagc
    19021 acgggaccca cgctccgctc gctcttggtg tacccggcgt cctcggcgtt ggtggagtcg
    19081 gacagcagca ggtgcacgcc gtggccggcc tcctcggcca gggcgtgcag gtccgtgggc
    19141 cggccgtcca gcggggtctg gtcgatcttg aagtcgccgg tgtgcaggat cgtgccgaac
    19201 ggcgtgtcga tcgccaccgc catcccgtcg gggatcgagt gggtgacccg catgaagcgc
    19261 atcgcgaacg gtccggccac ggtcgcctcg cccgggacca cctggcggaa ctcggcccgg
    19321 tcccggacgc cgtgctcctc cagcttgccc tccagcaggg ccagggtgaa cggcgtcccg
    19381 tacacgggga cggcccgctc cacctcgcgc agcaggtacg gcatggcccc gatgtggtcc
    19441 tcgtgcccgt gggtgagcac gatcgcctcc agccggtcca ggtgctccac cacgtactcg
    19501 aagtccggca gcaccaggtc gatgcccggc atctcggcgt gggggaagga cagccccacg
    19561 tcgaccacca gcatccggcc ggccagctcg acgcaggcca tgttccggcc cacctcgccc
    19621 acgccgccca aaaagaccac gcgggccggc ccgtcccccg tggcggggac ggggaccgct
    19681 ctagaggagg ccggcatcca cgagcgcctt ctggatcctg gcccgctcct cggaggtggc
    19741 cgggaccagg ggcaggcgcg ggggccccgc ggggagcccc agcagctgca gggcggtctt
    19801 gaccgggatc gggttcgtgg tgaggaacag cgtgttgaac aggggcgtga gctcctcgtg
    19861 caccttccgc gccgccgcga tgtcgccgga ggacaccagg tcggtgagct ggtggacccg
    19921 ctccccgatg atgttcgcgg ccaccgagac cacccccacg gcgcccaggc acatgtagcc
    19981 gaacgtggcc cagtcgtcgc ccgagtagac ctcgaagtcg gcgggggcct cgccgatcag
    20041 ccgggagatc gcctggaagt cgccggtgga gtccttgacc cccacgatgt tggggacctt
    20101 ggtcatctcc agcagcgtgt cgtgctcgat ccgcgtggcg gtgcgaccgg ggatgttgta
    20161 ggccacgatc ggcaggtcca cggccgaggc cacccgggtg aagtgctcga gcagaccccg
    20221 ctggggaggc ttgttgtagt agggggtcac cagcaggagg ccgtccgcgc cgagctgctc
    20281 cgcctcccgc gacagctcga tcgtctcccg cgtgttgtag gtgccggtcc cgcacaccac
    20341 cctgccccgg ccctcggcgg cctcgatcac cgcgcggaac aggtcggact tctcccggtg
    20401 cgacagcgtg ggcgcctcgc ccgtggagcc ggccaccacc agggtgtcgg tcccgtgctc
    20461 gagcaggtga cgggccagct cccgcgcccc ctccaggtcg agcgtgtggt cctcctggaa
    20521 cggcgtgacc atggcggtgg cgaccgcccc gaacctaccg gccatgtctc ctcctcaggt
    20581 gccgggtgcg acgggccggg gtgccgagca cgggacgccg ggcaccatcc aacgcgtcgc
    20641 acaagcgtca gaggagccac atgtggcacg tgacatgccg gttgctccag cacctcccgt
    20701 gatgcatgtg ctccacgagg tggaaccgcg ggccctccac cccccg
//
LOCUS       DASQQQ010000004        13447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_138032, whole genome shotgun sequence.
ACCESSION   DASQQQ010000004 DASQQQ010000000
VERSION     DASQQQ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13447
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138032"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VE646_00185"
     CDS             <1..420
                     /locus_tag="VE646_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017591277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HZD78449.1"
                     /translation="IQTVGRAARNARGTAIMYADRITGSMEYCIDETNRRRAVQEAYN
                     REHGIDPQSIRKKVSDILLSMSVDADKAPAPKRERRRRGVPEMPTEELERLIRSLEEE
                     MHEAAKDLRFEYAARLRDEVQDLRRELEGLRRAGVGS"
     gene            492..1457
                     /locus_tag="VE646_00190"
     CDS             492..1457
                     /locus_tag="VE646_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZD78450.1"
                     /translation="MCDGDRVGWVGTTGLEPPADEELEVDGFLMPAVADRHVHLGLAD
                     PAAVLMGGVAAVRDLAWPPDAIFQLADASELSTFNGPLIRAAGPMLTAPGGHPTQDAW
                     APDGTGLEVAGARQAEREVGALAGRGAAAIKVSLNAEAGPTVSDAELTAIVEAAHRHR
                     LPVTVHAQGRGQVQRALGAGVDELAHTPWTERLSDGLAETLARRTRIVSTLDIWSHGQ
                     VTAELRTAAENLARFRAAGGTVVYGTDLGSGSIPPGIDVREAMLLHETVGLSTDEVLA
                     SMVATSLQVGAPADLIACSRDPLEDLSALGDLALVVRMGRVVRRA"
     gene            complement(1460..2320)
                     /locus_tag="VE646_00195"
     CDS             complement(1460..2320)
                     /locus_tag="VE646_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017834634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZD78451.1"
                     /translation="MRSPIRAYPGTLARLSALDQLAEPLAGAIARLFGGGTVKEVVSG
                     TWLGHPAHPMLTDVTIGSWTSALLLDVLGGRRGRRAADRLIALGVLSAIPTAATGLSD
                     WADTTGTTRRVGMVHGMGNTAALMTFAASWLARKRGARLKGTALGLLGMGIGTATAYL
                     GGHLVYERGVGVNVTAFETPPSEWTAVAEEAQLAEGQPISVTAGPVEVLLVRSDGQIH
                     AIADRCSHLGGPLHQGSFQDATVTCPWHGSVFSLETGELVSGPARTPQPRYETRVNEG
                     RVEIRPVTEA"
     gene            complement(2423..2755)
                     /locus_tag="VE646_00200"
     CDS             complement(2423..2755)
                     /locus_tag="VE646_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78452.1"
                     /translation="MTDRAELERLSSQQLHDRATRLAWRKLDVAFLWDLLKTIPEARA
                     AIGDEARSRTDIMRPLALLNDWIDADQGELADALRPLYVDYLETHERPEDIQDTTRPP
                     SEGPSVRG"
     gene            2825..5692
                     /gene="uvrA"
                     /locus_tag="VE646_00205"
     CDS             2825..5692
                     /gene="uvrA"
                     /locus_tag="VE646_00205"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012087350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZD78453.1"
                     /translation="MGTDKLVIHGAREHNLRNVTLELPRGELIVFTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMEKPDVDFIEGLSPAISIDQKSTSKNPRSTVGT
                     ITEIYDYLRVLFARIGHPHCPRCGRPIGRQTPEQIVDQVMQLPEGTRFQILAPVIRGR
                     KGEYEKLLLDLARKGFVRARIDGEVRDLAEPIRLPRNYKHTIEVVVDRLVARPDIRRR
                     VADSIETALQLTEGVAAVAVQTHEGAEDVQTFSQNLACTFCGLSFDELQPRNFSFNSP
                     YGACPTCDGLGTRLEVDPELVVPDRDLSIREGAMAPWSGSRLEYWDRVLEAVAEAHGF
                     STSTPWKRLPKKARDILLYGSDEEIFVRYKNRYGRIRSYWTTYEGVVPSVERRHAETD
                     SDSQRDKLEQFMREVPCRACGGARLKPETLAVTVGDLNIAQLTARSIRDTLRFTDSVE
                     LSEREQMIAERLLKEIRARLGFLVDVGLDYLTLDRASATLAGGEAQRIRLATQIGSGL
                     VGVLYILDEPSIGLHQRDNRRLIDTLIRLRDLGNTLIVVEHDEATIQAADHLVDIGPA
                     AGSQGGEIVYSGDLKGLLECEASLTGAFLAGRRVIPTPEVRRSPADRWLKVVGAREHN
                     LKDVSLEIPLGLLICVTGVSGSGKSTLVQEVLLRALMQKIYRSRRLPGKHRRLVGWEQ
                     IDKVIDIDQSPIGRTPRSNPATYTGVFDHIRALFSQVPEARVRGYKPGRFSFNVRGGR
                     CENCAGDGQIKIEMHFLPDVYVTCEVCKGHRYNRETLEVRYKHRSIADVLEMSVDEAL
                     EFFQNIPVIKRHLQTLSDVGLGYVKLGQPAPTLSGGEAQRIKLAGELQKRATGNTFYM
                     LDEPTTGLHFEDIRKLLNVLQRLVSQGNTVLVIEHNLDVVKTADWIVDLGPEGGDGGG
                     RIVSTGPPEAVAADTASYTGQFLAEVLGTLPLEPERVHQDA"
     gene            5772..7748
                     /locus_tag="VE646_00210"
     CDS             5772..7748
                     /locus_tag="VE646_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HZD78454.1"
                     /translation="MTKRLLPVAFVTVLGLFAAACSKPPQATISVGAASSFVPQVADH
                     QDDVGLFPSVATDQQGTPYVAYFGFPQKLAEGQIAAPRPIGSPSVPSVDLVSVDQGIW
                     TRGAVAMEKDISNVTVPFGPAVVPQVKDLTPDNANGTSIAVDDTGGFHVAWVDGHGVW
                     YAQSSGSSFSASEVQRLKPPAKQAGPLGAPSVAVVDGTPWVTYVMSSAKGDRVTVAHQ
                     QGTTWKRDVLTTVPAGGSPPPRAAIAAAGNEPVVLFADGSGVRAATLGAGQEGGWAIT
                     DVEGGATGVGISLTSADDGLHAAYYADGEVHTATSKDGSSWQVGTVGSVGSGGDQAGR
                     STGIGVDSSGAVYVTWYDPGSDSVALASASDGSFSPLATRGTRGGQMPSLAVTPDGSA
                     VYVAWYDPQVQDLMVGTYGDTGGLALAQASPTPTGGAAATTPPPSNAGGECTQTQNGK
                     LTVTAEGIAFDTSCIQIPAGQKVTITFDNKDAGVQHNIAVFPSENDLTKPMFRGDLVT
                     GPDSVDYTVGPFDAGTYYFHCDVHPTMNGTFKVVSAGGGGGGSSTGGGQGGGGAAQYT
                     SAVSAKGIQFDTSTITLPANKATTLTFDNQDAGIQHNIAIFPSESDLTNPLFRGDLVT
                     GPNKVEYQIPALKPGTYYFHCDVHPTMNGQVVVK"
     gene            7807..9684
                     /gene="uvrC"
                     /locus_tag="VE646_00215"
     CDS             7807..9684
                     /gene="uvrC"
                     /locus_tag="VE646_00215"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005455693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZD78455.1"
                     /translation="MSVPRPETSTIPDLPGAYLFRDADGRVVYVGKARSLRKRLTTYW
                     GKPLHPRTEAMMAAAASVEWIVASTEVDALMLEYNLIQEHRPRFNVRYRDDKSYPYLA
                     LTVGERWPRAQVLRGSKRKNIRYFGPFGHVYAIRETLDALTRVFPVRTCSNAFFDQRA
                     RAQRPCLYYDIGRCAGPCVPQATGVTQSSYREHVEALADFLAGNERPVLQRLEREMRE
                     AAERQEYELAAKFRDQLVAARRALESQEMVLTQPQDLDVIGLAEDDLEAALQVFFVRR
                     GRVMGRKGWVVDRVEELDRAQLVASFVQQLYMEREEVPPRVLVPVLPADQEVLTDWLV
                     SRRRGPVRFRVPERGAKRKLLEVVGQNAREHFQRHKLRRASDFGARSRALSELAAVLG
                     LEQAPLRIECYDVSNLGPTDTVGSMVVFEDGLPKRSDYRRFGIKGVPGQDDFASMEEM
                     LRRRFARLRSERDGDVSRPRRFAYPPSLLVVDGGRGQLSVATRVLAEADLHIPAVGLA
                     KRLEEVYFPDRPDPLRIPRGSEALFVLQHIRDEAHRFAVTYHRGKRAKRQTASPLDGV
                     PGIGPARKKALIRRFGSLARLSRASLEEIEATPGMGPELARAVHEQLHATVGAERRES
                     A"
     gene            9684..10607
                     /gene="rapZ"
                     /locus_tag="VE646_00220"
     CDS             9684..10607
                     /gene="rapZ"
                     /locus_tag="VE646_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HZD78456.1"
                     /translation="MSQPAERERPPGRRVSPLHPAPPGPRFTIITGLSGAGRSESAKC
                     LEDLGFFVVDNLPPALLPKMAELVAKPGAPGRVAIVVDVRGGVFFGELSKALEDLKRL
                     NIPYRILYLEAADEDLVNRYAATRHRHPLAPADRVIEGIRKERLMMESLRGDADLIVD
                     TSGLTPHELRDRIRDAFSEAPPEQGLQVSLISFGYKYGAPRDADLVLDVRFLPNPHWV
                     DDLRPLPGTDERVRTYVKGQQTYREFMRRLRSLLGFMIPGYVAEGKSYLTVAVGCTGG
                     RHRSVVITDELAGFFRDKGMPATVEHRDLDR"
     gene            10726..11733
                     /gene="gap"
                     /locus_tag="VE646_00225"
     CDS             10726..11733
                     /gene="gap"
                     /locus_tag="VE646_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HZD78457.1"
                     /translation="MGVKVGINGFGRIGRNFFRAAKRRGMDFDLVAVNDITDAETLAH
                     LLRYDSVLGTMEADISVSEDGLTVDGDRIKVLAERDPGNLPWKDLGARIVLESTGLFR
                     KREDADKHIAAGAEKVVISAPAKGEDLTVVLGVNDDMYDPQAHNVISNASCTTNCVAP
                     MAKVLDDAFGIEQAFMTTIHAYTNDQRILDLPHKDLRRARAAAVNIIPTSTGAARATG
                     LVLPQLKGKMDGMAMRVPVPDGSVTDLVATVRGTPSVDEVNAAFQAAAGSGSLAGKLF
                     YTDDPIVSSDIVGSEASCTFDALSTMVSGNVVKIVGWYDNEWGYSNRLVDLVQMIADK
                     L"
     gene            11737..12936
                     /locus_tag="VE646_00230"
     CDS             11737..12936
                     /locus_tag="VE646_00230"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZD78458.1"
                     /translation="MPLRTLDTLGDVAGKRVFVRVDYNVPLEGGSVADDARIRASLPT
                     LTELLDGGASLVLASHLGRPKGRVEEELRLAPVADRLGELLGREVGALDQVVGAEVEG
                     ACGHLSGGEVVLLENLRFEPGEEQNDPAFAGRLAALADGYVNDAFGAVHRAHASVAAL
                     PALFLEQGRPAAAGRLLQREVEALSRLLEDPARPYVAVLGGAKVSDKLSTIGALIERV
                     DALLIGGAMAFTFIAADGGRVGDSLVEADRFDEVRSARARANERGVLIQLPDDVIAAP
                     EVSLEAKRQTVPASQVPTGQKGLDIGPRSVEEFARSMADAATILWNGPMGVFELEPFS
                     SGTRGVATAIAGSKAYTVAGGGDSLLAIRRFGLEDGFDHLSTGGGASLEFLEGRELPG
                     VKVLEVD"
     gene            12936..>13447
                     /locus_tag="VE646_00235"
     CDS             12936..>13447
                     /locus_tag="VE646_00235"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019853713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZD78459.1"
                     /translation="MARRPIIAANWKMHKTHLEAIRDVQRLSYLLDRDDAERVEVVIC
                     PAFTALRAVQTLIESDRLPYGLGAQDVHWEEQGAYTGEVSPPMLQALKCSYVIVGHSE
                     RRKDFGETDEAVTRKVRAVLAHEMTPILCVGETLEERDAGATTDKVAGQVQRALQGLG
                     AEDAGRLVVA"
BASE COUNT         2000 a         4690 c         4831 g         1926 t
ORIGIN      
        1 atccagacgg tcggacgcgc ggcgcgcaac gcgcgcggca cggcgatcat gtacgcggac
       61 cggatcaccg gctccatgga gtactgcatc gacgagacga atcgccgccg cgcggtgcag
      121 gaggcgtata accgcgagca cggcatcgat ccgcagtcga tccgcaagaa ggtctcggac
      181 atcctgctca gcatgtccgt cgacgcggac aaggcgcctg cccccaagcg cgagcgccgc
      241 cgccgcggcg tgcccgagat gcccaccgag gagctcgagc ggctgatccg gagcctggag
      301 gaggagatgc acgaggcggc gaaggacctc cggttcgagt acgccgcccg cctccgcgac
      361 gaggtccagg acctgcgccg ggagctcgaa gggctccgcc gggcgggggt cgggtcctga
      421 gcccgtccct ggcgctgcgc gccccccgcg cctggctcgg ccccggcaag accctcgagc
      481 gcgttacggt ggtgtgcgac ggcgaccggg tgggatgggt cggcaccacg gggttggagc
      541 cgccggcgga cgaggagctc gaggtggacg ggttcctgat gccggccgtg gccgaccgcc
      601 acgtgcacct cggtctggcc gatccggccg ccgtgctgat gggcggcgtg gcggcggtcc
      661 gcgacctggc gtggccgccc gatgccatct tccagctggc cgacgcctcg gagctgtcga
      721 ccttcaacgg gccgctgatc cgagcggcgg ggccgatgct cacggccccc ggtggccacc
      781 ccacccagga tgcctgggcg ccggacggca ccggactcga ggtggcgggc gcccggcagg
      841 ccgagcgcga ggtgggagcc ctggcagggc gcggcgccgc cgccatcaag gtctcgctga
      901 acgccgaggc cggtcccacc gtgagcgatg ccgagctcac ggcgatcgtg gaggcggccc
      961 accggcaccg gctcccggtc accgtccacg cgcagggccg aggccaggtc cagcgggcgc
     1021 tgggggccgg cgtggacgag ctggcgcaca ccccgtggac ggagcgcctc tccgacggcc
     1081 tggcggagac cctggccagg cgaacccgga tcgtgtccac gctcgacatc tggagccacg
     1141 ggcaggtgac ggcggagctc cggaccgcgg cggagaacct ggcgaggttc cgagcggcag
     1201 gaggcaccgt ggtgtacggg accgacctgg gctccggctc catcccgccg ggcatcgacg
     1261 tgcgagaagc catgctcctg cacgagacgg tcggcctgtc caccgacgag gtcctcgcat
     1321 ccatggtggc cacgtccctc caggtcggag cgccggccga cctgatcgcg tgctcgcgcg
     1381 accccctgga ggacctgtcg gccctggggg atctggcctt ggtggtccgg atgggacggg
     1441 tggtccggcg ggcctgagct caggcctcgg tgaccgggcg gatctcgacc ctcccctcgt
     1501 tgacccgggt ctcgtaccga ggttgggggg tccgggccgg gccgctcacc agctcgcccg
     1561 tctccaggct gaacacgctc ccgtgccacg ggcacgtcac ggtggcgtcc tggaacgacc
     1621 cctggtggag cggcccgccg aggtgcgagc agcggtcggc gatcgcgtgg atctgcccat
     1681 cggagcggac cagcagcacc tccaccggtc cggccgtgac cgagatcggc tggccctctg
     1741 ccagctgggc ctcctccgcc acggccgtcc actccgaggg cggcgtctcg aaggcggtga
     1801 cgttcacgcc caccccgcgc tcgtacacga ggtgtccgcc caggtacgcc gtggccgtgc
     1861 cgatccccat ccccagcagt ccgagagccg tgcccttcag ccgggcaccg cgcttgcggg
     1921 cgagccagga ggcggcgaag gtcatcaggg ctgccgtgtt ccccatcccg tggaccatgc
     1981 ccacccgacg cgtggtcccg gtggtgtcgg cccagtccga cagcccggtg gccgcggtgg
     2041 ggatggcgct caggacgccc agggcgatca gccggtccgc ggcccgccgg ccccgccgtc
     2101 cccccagcac gtcgagcagg agggccgacg tccacgaccc gatcgtgaca tccgtcagca
     2161 tcgggtgggc tggatggccc agccacgtcc cgctcacgac ttccttcacg gtgccgcccc
     2221 cgaacagcct ggcgatggcg ccggcgagcg gttccgccag ctggtccagg gccgagagcc
     2281 gagccagggt gccggggtat gcgcggatcg gggacctcat gggacctcct ccttccggga
     2341 ccgtcctccc cgttgttccc ggcgagccga ggaggtactc ctggtgcccg gcgggcccgg
     2401 gatctcctct cgggccggac ggctatccgc gaaccgaggg gccctcgctg ggagggcggg
     2461 tggtgtcctg gatgtcctcg ggacgctcgt gcgtctccag gtagtccacg tacagggggc
     2521 gcagcgcgtc ggccagctcg ccctggtcgg cgtcgatcca gtcgttcagc agggccagcg
     2581 gccgcatgat gtccgtgcgg ctccgagcct cgtccccgat cgccgctcga gcctcgggga
     2641 tcgtcttgag caggtcccag aggaaggcca cgtccagctt cctccaggcc aggcgggtgg
     2701 cccggtcgtg gagctgctgg gaggagaggc gctcgagctc ggcccggtcg gtcatggcgc
     2761 cccagcctat cgcgccccga ggacgggtgg cggcgctcgt cggcggccca actacaatgc
     2821 ctccgtgggc accgacaagc tcgtgatcca tggtgcccgt gagcacaacc tcaggaacgt
     2881 caccctcgag cttccccgtg gcgagctgat cgtgttcacg ggcctgtccg gctccggcaa
     2941 gagctccctg gccttcgaca cgatctacgc ggagggtcag cgccgctacg tggagtcgct
     3001 gtccgcctac gcccgtcagt tcctgggcca gatggagaag ccggacgtcg acttcatcga
     3061 aggcctgtcc ccagccatct cgatcgatca gaaatccacc tcgaagaacc cgcgctccac
     3121 ggtgggcacc atcaccgaga tctacgacta cctgcgggtc ctgttcgcgc ggatcggtca
     3181 tccgcactgt cctcgatgcg gtcgccccat cggccggcag acgccggagc agatcgtcga
     3241 ccaggtgatg cagctgccgg aggggacccg gttccagatc ctcgcgccgg tgatccgggg
     3301 gcgcaagggc gagtacgaga agctcctgct ggatctggcg cgcaagggct tcgtccgggc
     3361 tcggatcgat ggcgaggtcc gggacctggc cgagcccatc cgtctgcccc gcaactacaa
     3421 gcacacgatc gaggtggtcg tcgatcgcct ggtggcccgg cccgatatcc gccgccgcgt
     3481 ggcggactcc atcgagaccg ccctgcagct gaccgagggt gtcgcggcgg tggccgtcca
     3541 gacgcacgag ggggccgagg acgtccagac cttctcccag aacctggcct gcaccttctg
     3601 cgggctgtcg ttcgacgagc tgcagccccg gaacttctcc ttcaactcgc cctacggggc
     3661 ctgtcccacg tgcgacggcc tgggcacccg gctcgaggtg gatcccgagc tggtggtgcc
     3721 ggaccgggac ctgtcgatcc gggagggcgc gatggccccg tggtcgggct ctcgcctcga
     3781 gtactgggat cgcgtcctgg aggccgtggc cgaggcgcac gggttctcca cctccacgcc
     3841 ctggaagcgg ctgccgaaga aggcccggga catcctgctg tacggctcgg acgaggagat
     3901 cttcgtccgg tacaagaacc gctacggtcg catccgctcc tactggacca cgtacgaggg
     3961 cgtcgtcccg agcgtggagc gccggcacgc cgagaccgac tcggacagcc agcgggacaa
     4021 gctcgagcag ttcatgcgcg aggtgccctg ccgggcctgt ggcggggccc ggctgaagcc
     4081 ggagaccctg gcggtcaccg tgggcgatct gaacatcgcc cagctcaccg cccggtcgat
     4141 ccgggacacg ctccggttca ccgactcggt cgagctgtcc gagcgggagc agatgatcgc
     4201 ggagcgactg ctgaaggaga tccgggcccg ccttgggttc ctggtggacg tggggctgga
     4261 ctacctcacg ctcgaccggg cctctgccac gctggccgga ggcgaggcgc agcgcatccg
     4321 gttggccacg cagatcggct cgggcctggt gggggtgctg tacatcctcg acgagccctc
     4381 gatcggcctg caccagcggg acaaccgccg gctgatcgac acgctgatca ggctccgcga
     4441 tctgggcaac acgctgatcg tggtcgagca cgacgaggcc acgatccagg ccgccgacca
     4501 cctggtggac atcggtccgg cggcggggag ccagggcggc gagatcgtct actccgggga
     4561 cctgaagggt ctgctggagt gcgaggcctc gctcaccggg gcgttcctgg ccggtcggag
     4621 ggtgatcccc acccctgagg tgcgccgcag ccccgccgac cggtggctga aggtggtcgg
     4681 cgctcgggag cacaacctga aggacgtgag cctggagatc ccgctcgggc tgctgatctg
     4741 cgtgacgggg gtctccgggt ccggcaagtc caccctcgtg caggaggtcc tgctccgggc
     4801 gctgatgcag aagatctacc ggtcccgacg cctgcccggc aagcaccgcc gcctggtcgg
     4861 gtgggagcag atcgacaagg tgatcgacat cgaccagtcg cccatcggac gcaccccccg
     4921 ttcgaacccc gccacgtaca cgggcgtgtt cgaccacatc cgtgcgctgt tcagccaggt
     4981 gcccgaagcg cgggtgcgtg ggtacaagcc ggggcgcttc tcgttcaacg tccgcggcgg
     5041 gcggtgcgag aactgcgccg gcgacgggca gatcaagatc gagatgcact tcctgcccga
     5101 cgtctacgtg acctgcgagg tctgcaaggg gcaccgctac aaccgggaga ccctcgaggt
     5161 ccggtacaag caccggtcga tcgccgacgt cctggagatg agtgtggacg aggccctcga
     5221 gttcttccag aacatcccgg tgatcaagag gcacctgcag acgctgtccg atgtgggtct
     5281 cgggtacgtg aagctcgggc agcctgctcc caccctgtcc gggggcgagg cgcagcgcat
     5341 caagctggcc ggcgagctgc agaagcgcgc caccggcaac accttctaca tgctggacga
     5401 gcccaccacc gggctccact tcgaggacat ccggaagctg ctcaacgtct tgcagcgctt
     5461 ggtgtcgcag ggcaacacgg tcctggtgat cgagcacaac ctggacgtgg tgaagacggc
     5521 cgactggatc gtggatctgg ggcccgaggg cggggacggc gggggtcgga tcgtcagcac
     5581 cgggcccccc gaggcggtgg ccgccgacac cgcctcgtac acggggcagt tcctggccga
     5641 ggtgctcggg accctgcccc tggagcccga gcgggtccat caggacgcgt gagcggaggc
     5701 ccgcgccgct cgccaggtca tcgcgccagg ccgcacgcca gcgggcttcc ttgctagcat
     5761 tgcgcccgct catgaccaag aggctgcttc cggtcgcgtt cgtcaccgtc ctgggactgt
     5821 tcgcggctgc gtgctccaag ccgccgcagg ccaccatctc cgtgggcgcc gcgagctcct
     5881 tcgtgccgca ggtggccgat caccaggacg atgtgggcct gttcccgtcg gtggccacgg
     5941 accagcaggg cacgccctac gtcgcctact tcgggttccc ccagaagctc gccgagggcc
     6001 agatcgcggc gcctcggccc atcggatccc cctcggtccc aagcgtggac ctggtgagcg
     6061 tggatcaggg catctggacc cgcggcgccg tggccatgga gaaggacatc tccaacgtga
     6121 ccgtgccctt cggcccggcc gtggtgccgc aagtgaagga cctgacaccg gacaacgcca
     6181 acggcacctc catcgccgtc gacgataccg gcggcttcca cgtggcctgg gtcgacgggc
     6241 acggggtctg gtacgcacag agctcggggt cctcgttctc ggcgtccgag gtacagaggc
     6301 tgaagccgcc cgcgaagcag gccgggccgc tcggcgctcc ctcggtggcc gtcgtggacg
     6361 gcacgccgtg ggtgacgtac gtgatgagct cggccaaggg cgatcgggtc accgtggcgc
     6421 accagcaggg caccacctgg aagcgcgacg tgctcaccac ggtccccgcc ggcggctcac
     6481 cgccgccacg agccgcgatc gccgcggccg ggaacgaacc cgtggtgctc ttcgccgacg
     6541 gctccggggt ccgcgcggcc acgctcgggg cgggccagga gggcggctgg gcgatcacgg
     6601 acgtcgaggg cggtgccacg ggcgtgggga tctcgctgac ctcggccgat gacggcctgc
     6661 atgccgccta ctacgccgac ggggaggtgc acacggccac gtcgaaggac ggcagctcgt
     6721 ggcaggtcgg cacggtgggc tccgtgggca gcggtgggga tcaggccggc cgctccacgg
     6781 ggatcggcgt cgacagctcc ggcgccgtct acgtgacctg gtacgacccc gggtccgact
     6841 ccgtggcgct cgcctcggcc tccgacggca gcttctcccc cctggccacg cggggcaccc
     6901 ggggcggcca gatgccgtcg ctggcggtga cccccgacgg ctccgccgtc tacgtggcgt
     6961 ggtacgaccc gcaggtccag gacctgatgg tcgggaccta cggcgatacg ggcggcctgg
     7021 ccctggcgca ggcgagcccc acgcccacgg ggggcgcggc ggccaccacg ccgccgccct
     7081 cgaacgccgg cggggagtgc acgcagactc agaacggcaa gctcaccgtg accgcggagg
     7141 gcatcgcctt cgacaccagc tgcatccaga tcccggccgg ccagaaggtc acgatcacct
     7201 tcgacaacaa ggacgccggt gtccagcaca acatcgcggt cttccccagc gagaacgacc
     7261 tcacgaagcc catgttccgg ggcgacctgg tgacgggacc ggactcggtc gactacacgg
     7321 tcgggccctt cgacgccggc acctactact tccactgcga cgtgcatccc acgatgaacg
     7381 ggacgttcaa ggtcgtctcc gccggtggtg gtgggggtgg atcgtccacc ggcggcggtc
     7441 agggtggcgg aggtgccgcc cagtacacga gcgccgtatc cgccaagggg atccagttcg
     7501 acacgagcac catcaccctg ccggcgaaca aggcgaccac gctgaccttc gacaaccagg
     7561 acgccgggat ccagcacaac atcgcgatct tcccgagcga gagcgacctg accaacccgc
     7621 tcttccgggg cgatctggtg accgggccca acaaggtcga gtaccagatc cctgcgctga
     7681 agccgggcac ctactacttc cactgcgacg tgcaccccac catgaacgga caggtggtcg
     7741 tgaagtagtc gtgcggatcc ggggggggtc ccggttcccg caggtcacga cgctaggatg
     7801 ggacgcatga gcgtccctcg tcccgagacc tccacgatcc ccgacctgcc cggcgcctac
     7861 ctgttccgcg acgcggacgg tcgagtggtc tacgtgggca aggcccggtc gctccgcaag
     7921 cgcctgacca cgtattgggg caagccgctg cacccccgga ccgaggcgat gatggcggcg
     7981 gccgcctcgg tggagtggat cgtggcctcc accgaggtcg acgcgctgat gctcgagtac
     8041 aacctgatcc aggagcaccg gccccgcttc aacgtccggt accgggacga caagtcctac
     8101 ccgtacctgg ccctcacggt gggagagcgg tggccccggg cgcaggtcct gcggggttcc
     8161 aagcggaaga acatccggta cttcggcccc ttcgggcacg tgtacgcgat ccgggagacg
     8221 ctggacgctc tcacccgggt cttcccggtc cggacctgct cgaacgcgtt cttcgaccag
     8281 cgggcgcgag cccagcgccc ctgcctctac tacgacatcg gccgctgcgc cgggccatgc
     8341 gtcccccagg ccaccggggt gacccagagc tcgtaccggg agcacgtgga ggccctggcc
     8401 gacttcctcg ccggcaacga gcgcccggtc ctgcagcggc tggagcgcga gatgcgggag
     8461 gcggcggagc ggcaggagta cgagctggcc gcgaagttcc gcgatcagct ggtcgccgcg
     8521 cggcgggccc tggagagcca ggagatggtg ctcacccagc cgcaggatct ggacgtgatc
     8581 ggcctggccg aggacgacct ggaggccgcc ctccaggtgt tcttcgttcg ccgggggcgg
     8641 gtgatggggc gcaagggctg ggtggtggac cgggtggagg agctcgaccg tgcccagctg
     8701 gtggcctcgt tcgtccagca gctctacatg gagcgcgagg aggtccctcc ccgggtgctc
     8761 gtccccgtgc tccccgccga ccaagaggtc ctcacggact ggctggtctc tcgccggaga
     8821 gggccggtgc ggttccgggt cccggagcgg ggcgcgaagc gcaagctgct cgaggtggtc
     8881 ggccagaacg cccgggagca cttccagcgc cacaagctcc gccgagcctc ggacttcgga
     8941 gcccgctccc gcgcgctgtc ggagctggcc gccgtgctgg ggctcgagca ggcccccctg
     9001 aggatcgagt gctacgacgt ctcgaacctg gggccgaccg acacggtcgg atccatggtg
     9061 gtcttcgagg acgggctgcc gaagcggtcg gattaccgga ggttcggcat caagggggtc
     9121 cccggacagg acgatttcgc cagtatggag gagatgctgc ggcgccggtt cgctcgtctc
     9181 cggtccgagc gggacgggga cgtctccagg ccacggcggt tcgcgtaccc accgtccctg
     9241 ctggtcgtcg acggggggcg tggccagctg tccgtggcca cccgggtgct ggccgaggcc
     9301 gacctgcaca tcccggccgt ggggctggcc aagcgcctgg aggaggtcta cttccccgac
     9361 cgaccggacc cgctccggat cccgcgcgga tccgaagccc tcttcgtgct ccagcacatc
     9421 cgggacgagg cgcaccgctt cgccgtcacc taccatcgcg gcaagcgggc caaacgccag
     9481 accgcctccc ccctggacgg ggtgcccggt atcggtcccg cgcgcaagaa ggcgctgatc
     9541 cgccggttcg gatcgctggc ccggctctca cgtgcgtccc tcgaggagat cgaggccacg
     9601 ccggggatgg gtcccgagct cgcccgggcc gtgcacgagc agctccacgc gacggtgggc
     9661 gccgagcgga gggagagcgc gtgatgtcgc agccagccga gcgggagcgt ccgccggggc
     9721 gccgggtctc tcccctgcac cctgcccctc cgggcccccg cttcacgatc atcaccggcc
     9781 tgtcgggcgc tgggcggtct gagtcggcga agtgcctgga ggacctgggg ttcttcgtgg
     9841 tggacaacct gccccccgcc ctgctcccga agatggccga gctcgtggcc aaaccgggtg
     9901 ccccgggccg ggtggccatc gtcgtggacg tgcggggggg cgtgttcttc ggcgagctgt
     9961 ccaaggcgct cgaggacctg aagcgcctga acatcccgta ccggatcctg tacctggagg
    10021 ccgcggacga ggacctggtg aaccggtacg cggccacccg gcaccggcat ccgttggctc
    10081 ccgccgaccg ggtgatcgag ggcatccgca aggagcgcct catgatggag agcctccggg
    10141 gggacgccga cctcatcgtg gacacctccg ggctcacgcc ccacgagctg cgcgaccgga
    10201 tccgggatgc cttctccgaa gcgcctcccg agcagggcct gcaggtctcg ctgatctcct
    10261 tcggctacaa gtacggtgcg ccccgagacg cggacctggt gctcgacgtc cgcttcctgc
    10321 cgaacccaca ttgggtggac gacctgcggc cgctgccagg gaccgacgag cgcgtgcgca
    10381 cctacgtgaa gggccagcag acgtaccgcg agttcatgcg gcggctgcgg tcgctcctcg
    10441 gcttcatgat ccccggctac gtggccgagg ggaagtccta cctcaccgtg gccgtggggt
    10501 gtacgggagg acggcatcgc tcggtggtga tcaccgacga gctggcgggg ttcttccgcg
    10561 acaagggcat gccggccacc gtggagcacc gcgatctgga tcgctgaccc ggtcacggcg
    10621 ggcaccgcct ccgggccctg cgcgtgcccg tgccgaaacg gcccggaccg ggctacgcgg
    10681 cggattggct aggatgggtg caaccccccc gcgaggagga cgaccatggg cgtgaaggta
    10741 ggcatcaacg ggttcgggcg gatcgggcgc aacttcttcc gggccgccaa gcggcgcggc
    10801 atggacttcg acctggtcgc cgtgaacgac atcaccgacg ccgagacgct ggctcatctg
    10861 ctacggtacg actccgtcct ggggacgatg gaggccgaca tcagcgtctc cgaggacggc
    10921 ctcaccgtgg acggcgaccg gatcaaggtg ctcgccgaac gggacccggg gaacctgccc
    10981 tggaaggacc tcggagcgcg catcgtgctc gagtcgaccg gtctcttccg caagcgcgag
    11041 gacgccgaca agcacatcgc ggccggcgcg gagaaggtcg tgatcagcgc cccggccaag
    11101 ggagaagacc tcacggtcgt gctcggtgtg aacgacgaca tgtacgaccc gcaggcgcac
    11161 aacgtcatct ccaacgcgtc ctgcaccacg aactgcgtcg ccccgatggc caaggtcctg
    11221 gacgacgcct tcggcatcga gcaggccttc atgaccacga tccacgccta cacgaacgac
    11281 cagcgcatcc tcgacctgcc gcacaaggat ctgcgccgcg cccgtgccgc cgccgtgaac
    11341 atcatcccca ccagcaccgg cgcggccagg gccaccggtc tggtgctgcc gcagctcaag
    11401 ggcaagatgg acgggatggc catgcgcgtc ccggtgcccg acggctcggt cacggacctc
    11461 gtggccacgg tccggggaac gccctccgtg gacgaggtca acgccgcctt ccaagcggcc
    11521 gccgggagcg gatccctcgc cggcaagctc ttctacacgg acgacccgat cgtctccagc
    11581 gacatcgtcg ggagcgaggc gtcgtgcacg ttcgatgccc tctccacgat ggtctcgggc
    11641 aacgtggtga agatcgtggg ctggtacgac aacgagtggg ggtactcgaa ccgcctggtc
    11701 gacctggtcc agatgatcgc cgacaagctc tgagccgtgc cgctccggac gctcgacacg
    11761 ctcggcgacg tcgcggggaa gcgggtcttc gtgcgcgtgg actacaacgt gccgctggag
    11821 gggggctcgg tggccgacga tgcccgtatc cgggcctccc ttcccaccct caccgagctg
    11881 ctcgacggcg gcgcctcgct ggtgttggcc tcgcacctag gtcgccccaa ggggcgggtc
    11941 gaggaagagc tccggctggc tccggtggcg gatcgcctgg gggagctgtt gggtcgggag
    12001 gtgggtgccc tggaccaggt ggtgggtgcg gaggtcgagg gcgcctgcgg ccacctgagc
    12061 ggaggtgagg tcgtgctgct cgagaacctc cggttcgagc cgggggagga gcagaacgac
    12121 ccggccttcg ccgggcgcct ggccgcgttg gccgacgggt acgtgaacga cgccttcggg
    12181 gccgtccacc gtgcgcacgc gtcggtcgcg gcgctgcccg ccctctttct ggagcagggc
    12241 cgccccgcgg cggccggccg gctgctccag cgcgaggtcg aggcgctctc caggctgctg
    12301 gaggacccgg cccggcccta cgtcgccgtg ctcggcgggg cgaaggtgtc cgacaagctc
    12361 tccacgatcg gggccctgat cgaacgggtc gatgcgctgc tgatcggcgg ggcgatggcc
    12421 ttcacgttca tcgcggccga cggcggacgg gtcggcgaca gcctggtgga ggccgaccgg
    12481 ttcgacgagg tccggagcgc cagagcgcga gccaacgagc ggggggtgct gatccagctc
    12541 ccggacgacg tgatcgcggc tcccgaggtg tccctggagg cgaagcggca gacggtgccg
    12601 gcctcgcagg tccccaccgg gcagaagggc ttggacatcg ggccgcgctc ggtggaggag
    12661 ttcgcgagga gcatggcgga cgccgccacg atcctctgga acgggcccat gggggtcttc
    12721 gagctggagc cgttctcctc gggcacgcga ggggtggcca ccgccatcgc cggatcgaag
    12781 gcgtacacgg tggccggcgg cggcgacagc ctcttggcca tccgccgctt cggcctggag
    12841 gacgggttcg accacctgtc caccggcggg ggggcctccc tggagttcct ggagggccgt
    12901 gagctgcccg gcgtgaaggt cctggaggtc gactgatggc ccggcgaccg atcatcgcgg
    12961 cgaactggaa gatgcacaag acccacctgg aggcgatccg ggacgtgcag cggctctcct
    13021 acctgctcga ccgggacgac gccgagcggg tcgaggtggt gatctgcccg gccttcacgg
    13081 cgctccgggc ggtccagacc ctgatcgagt cggatcgtct cccgtacggc ctgggtgcgc
    13141 aggacgtgca ctgggaggag cagggtgcct acacgggtga ggtctcgccc cccatgctgc
    13201 aggcgctgaa gtgctcgtac gtgatcgtgg gtcattccga gcgccgcaag gacttcgggg
    13261 agaccgacga agctgtgacc cggaaggtcc gcgccgtgct ggcccacgag atgaccccga
    13321 tcctgtgcgt gggggagacg ctcgaggagc gcgacgccgg cgccaccacg gataaggtcg
    13381 cgggacaggt ccagcgagcc ctccagggcc tgggagcgga ggacgccggg aggctcgtgg
    13441 tggccta
//
LOCUS       DASQQQ010000005         7405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_55426, whole genome shotgun sequence.
ACCESSION   DASQQQ010000005 DASQQQ010000000
VERSION     DASQQQ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7405
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55426"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VE646_00240"
     CDS             complement(<1..346)
                     /locus_tag="VE646_00240"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HZD78460.1"
                     /translation="MTKPQIAVVAYHLRPGRVAMWLVGGYAVPENYIQAIRRGGGRPA
                     LLLPGDDTPPAELLAAFDGLVLVGGGDVDPRSFGQEPHTSVYGLEPDRDRFEIDLLLE
                     ADRTGTPTLAICR"
     gene            complement(343..1002)
                     /locus_tag="VE646_00245"
     CDS             complement(343..1002)
                     /locus_tag="VE646_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HZD78461.1"
                     /translation="MERSAFLARVRARLADGMPQSRAAPRSAPAAPTAPTPVPTMVSG
                     DGGGFERFAAELMQLGGQARLIASRDLADAVAASSIGCRRSVVGEDLGAFRSAITEGL
                     SASGCVVVPAGRDQAASADLGVTGGLWAVASTGSVLVAADPGAPRTTGLLPQRHLIVL
                     PEERLLPGLEELFEQLPDVVSETSAAVLVTGPSRTSDIEMISVRGVHGPGRVMVLLVS
                     T"
     gene            complement(992..2401)
                     /locus_tag="VE646_00250"
     CDS             complement(992..2401)
                     /locus_tag="VE646_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HZD78462.1"
                     /translation="MTDPDGTTFRSRADALLAEPAIGAAIGRAMDVRATARARVFRDA
                     DLEAMRAAGEAIRQHTIAGLAGYLDRFARNAEARGTRVFFASDASEAVGYVREVIRRA
                     GARSIVKSKSMVSEEIGLNAALASDGAEVVETDLGEYIVQLAHQPPAHITVPAVHMTR
                     TQIRDLFGREHGVSLSDEPGELTAFARQVLRERFLAADVGITGVNFAVADTGSLVIVT
                     NEGNARMCTSLPRIHIALLGMERLVPSFRELSVLLPLLTGSATGQRITAYVNVIAGPR
                     LPREADGPEEVHVVVLDNGRSAILGTEYRSILHCIRCGACQNACPVFRQVGGHAYGTT
                     YGGPIGAVLEPLLEGFDRAGDLPHASSLCLACTDVCPVNIPLHEHLISLRRDAVAIQA
                     SVLERLVFALWGRLWSRPRWYRMSARLARGAQRPFTRGGRIGRAPFPLSRWTSGRDLP
                     PLAGRSFQDRWARDRRRGT"
     gene            complement(2388..3164)
                     /locus_tag="VE646_00255"
     CDS             complement(2388..3164)
                     /locus_tag="VE646_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HZD78463.1"
                     /translation="MPGGTRPTRVSLFVTCLIDLLYPEIADATVRLLRDLDVEVDVPG
                     GQTCCGQPAYNAGFPGDARRMAGALIGAIGDGGTVVTSSGSCAAMVRSNLPTLFSGTP
                     HQERARGLAERTFELSQFLVDELGVEGRIGSLPARVTFHDACHGLRELGLASQGRRLL
                     AGIEGLELVEMARPDACCGFGGTFSVRLPEMATAMGADKLGQAGATGADLLVTGDAGC
                     LMHLAGLRSRRDRSPRPVHLAVLLAQARGLLAPEGPDDRP"
     gene            complement(3169..3888)
                     /locus_tag="VE646_00260"
     CDS             complement(3169..3888)
                     /locus_tag="VE646_00260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HZD78464.1"
                     /translation="MEPVLLMRNDRYETFGVAPTALAWAGLDVRTANLIEGASLPRLD
                     EVSGVIAFGGTANVDQVDRFPYLRGARDYTSRALDRGVPYLGICLGAQLLARAAGEEV
                     FAAPVKEVGFEPLRPTGEAKEDPLLSQYVDGDMVFQWHEDTYRLPAGATLLASGDRID
                     VEAFRVGDRAWGVQFHQELDALELGWWLEIAEAEMDVEATWGKSGDRIRGESARHMPA
                     HEERGRELFRRFAEVVRDASR"
     gene            complement(3941..4933)
                     /locus_tag="VE646_00265"
     CDS             complement(3941..4933)
                     /locus_tag="VE646_00265"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HZD78465.1"
                     /translation="MPERPSVLVTRLLPPPVMEVLRERSDLDVYRGEGAIPRDELLAA
                     VPGRQGVITLLTEKVDAGFLDAAGPTLKVVANYAVGYDNIDVDECTRRGVVASNTPDV
                     LTETTADLTWALLLSAARRIAEGDRFLRTGTPWIWGPQMLLGVDVHDATLGVVGFGRI
                     GQAVARRGRGFGMTVIYSDVVAAPEAEREFGATRVGFSELLARADFITINTALTPQTR
                     HLFGTEEFRRMKGTAVLANSARGPIVDEVALAAALRDGEIFAAGIDVFEEEPTVHPDL
                     VACENAVIVPHLGSASVDTRIAMGMLAVDNLLAVLEGRRPPTPLNPQVLERSGS"
     gene            complement(4926..6098)
                     /locus_tag="VE646_00270"
     CDS             complement(4926..6098)
                     /locus_tag="VE646_00270"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015142190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZD78466.1"
                     /translation="MELAARMGRLGTESAFEVLARAKALEAKGRQIVHLELGEPDFTT
                     PEHIRDAAKEALDRGMTHYVPAPGIPPLREAVAAFLERHGRLSPSPDRVVVTPGAKPI
                     MFFTILALCEEGDEVIYPDPGFPMYESIANFSGATPVPIPLREQNGFRLDPEELASLV
                     TDRTKLLILNSPHNPCGSALTKQDLEAVAEIALRHDIVVLSDEVYWMIRYESEHSSIL
                     DVEGMQERTVLLDGWSKTWAMTGWRLGFGVFPEYLVEPVTRLAINSVSCTSAFSQHAA
                     IAALTGPMEPSQRMVQEFRRRRDVIVDGLNAIPGVSCLTPDGAFYVFPNVSGLGRSSR
                     EVEAHLLEEAGVAALSGTAFGRYGDGYLRLSYATSLEDIEIALAAFQASLPELSRA"
     gene            6244..>7405
                     /locus_tag="VE646_00275"
     CDS             6244..>7405
                     /locus_tag="VE646_00275"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZD78467.1"
                     /translation="MASELMILGGERVPAADGTTYEVIEPGTGRPMARVSEAGTEDAA
                     SAARIAHRAFEEGAWRRMSGTQRGRVLLRAASLLRERSEQMATVESRNVGKPIRWARG
                     EVETVAGVLEYWGGAANKIFGETIPVSAPGLDLTLREPVGPCVLITPWNYPLVISSWK
                     IGPALACGNPMIVKPASLTPLSVLLLADVLVEAGLPPETISVLPGPGAPTGEALVRDP
                     LVAKVSFTGSTAVGVRVMQHGAEHITRVSLELGGKSANVVFADADMDRCIEESVWAVF
                     GNTGQDCTARTRLFVERPVYERFVDGLAARTQRLRIGPPTDEATELGPMISAAQRRTS
                     LEYLEVGRDEGARLVMGGDVPEELEEGFFLRPAILADASNDMRVAREEIFGPV"
BASE COUNT         1109 a         2621 c         2653 g         1022 t
ORIGIN      
        1 cgcggcagat ggccagggtt ggggtaccgg tgcggtcggc ctccaggagc aggtcgatct
       61 cgaagcggtc ccgatccggt tccagcccgt agacgctggt gtggggttcc tggccgaagg
      121 agcgggggtc cacgtcgccc ccgccgacca gcaccaggcc gtcgaacgcc gccagcagtt
      181 cggcgggtgg ggtgtcgtcg ccggggagca gcagggcggg ccgcccgccg cccctgcgga
      241 tggcctggat gtagttctcc gggaccgcgt agccgcccac cagccacatg gccacccgtc
      301 cggggcgcag atggtaggcg accacggcga tctggggctt ggtcacgtgc tcaccaggag
      361 caccataacg cgtccgggtc cgtggacacc ccggaccgag atcatctcga tatcgcttgt
      421 ccgggacggc ccggtgacca ggaccgccgc cgacgtctcg gagacgacgt ccggtagctg
      481 ctcgaacagc tcctccagac cggggaggag ccgctcctcg ggcaggacga tgaggtgacg
      541 ttgggggagc aggccggtgg tgcgtggcgc cccagggtcg gcggccacca ggaccgaccc
      601 ggtagaggcc acggcccaga ggccgccggt cacgccgaga tccgcggacg cagcctgatc
      661 ccgaccggcc ggtaccacca cgcacccgga ggccgacagg ccctcggtga tcgccgaccg
      721 gaaagctccg aggtcctcgc cgacgacgga ccgccggcag ccgatcgagg aggcggccac
      781 ggcatctgcg aggtcccgtg atgcgatgag ccgagcctgc ccccccagct gcatgagctc
      841 ggcagcgaac cgctcgaacc cccccccgtc gccggagacc atggtgggga cgggggtggg
      901 tgcggtcggc gcggccggtg ctgaccgcgg tgccgcgcgg ctctgcggca tcccgtcggc
      961 cagtcgagct cgaacgcgcg cgaggaaggc gctacgttcc acgccgtcga tccctcgccc
     1021 agcggtcctg gaagctccga ccggccaacg gaggaaggtc gcgacccgac gtccatcgcg
     1081 acagcgggaa gggggcgcga ccgatcctgc cgccccgcgt gaacggccgc tgtgcgcccc
     1141 gagcgagccg cgcgctcatc cggtaccacc tcggtctgct ccacagacga ccccacagcg
     1201 cgaacacgag ccgctcgagc acgctcgcct ggatggcgac cgcgtctcgg cggagcgaga
     1261 tcaggtgctc gtgcagcggg atgttcacgg ggcatacgtc cgtgcacgcc aggcagaggg
     1321 acgaggcgtg cggcaggtcg ccggctcgat cgaagccctc gagcaacggt tcgagcacgg
     1381 cgccgatcgg gcctccgtag gtggtcccgt acgcgtgccc gccgacctgt cggaagaccg
     1441 gacaggcgtt ctggcacgcg ccgcaccgga tgcagtgcag gatcgatcgg tactcggtgc
     1501 ccaggatggc ggagcgcccg ttgtcgagca ccaccacatg cacctcctcc gggccgtcgg
     1561 cttcccgggg caggcgaggc cccgcgatca cgttcacgta ggcggtgatg cgctgaccgg
     1621 tggcgctgcc cgtgaggagc ggcaggagca cggacagctc ccggaacgag ggcacgagcc
     1681 gctccatgcc caggagggcg atgtggatcc gtggcagcga ggtgcacatc ctggcgttcc
     1741 cctcgttcgt cacgatgacc agggagccgg tgtcggcgac ggcgaagttc acgccggtga
     1801 tccccacgtc ggcggccagg aagcgctcgc gcaggacctg gcgggcgaag gcggtcagct
     1861 cgccgggctc gtcggagagc gagacgccgt gctcgcgccc gaagagatcc cggatctgcg
     1921 tccgggtcat gtgcacggcg gggacggtga tgtgggcggg aggctggtgt gccagctgga
     1981 cgatgtactc gccgaggtcg gtctcgacga cctcggcacc gtccgaagcc agggcggcgt
     2041 tcagaccgat ctcctcggag accatcgact tgctcttcac gatcgagcgc gcgccggcac
     2101 ggcggatcac ctcgcggacg taccccaccg cctcggaggc atcggacgcg aagaacaccc
     2161 tggtgcctcg ggcctcggcg ttgcgggcga agcggtccag gtaccccgcc aggccggcga
     2221 tggtgtgttg gcggatcgcc tcccccgcgg cgcgcatggc ctccaggtcg gcatcccgga
     2281 agacgcgagc ccgggcggta gcgcggacgt ccatcgcgcg gccgatcgcc gcgccgatcg
     2341 caggctcggc caggagcgca tcggctcggc tccggaacgt ggttccgtca gggtcggtca
     2401 tcggggcctt cgggagccag cagacctcgg gcctgcgcca acaagacggc cagatgcacc
     2461 ggtcggggcg agcgatccct gcgggagcgc aggcccgcca ggtgcatcag gcaccccgca
     2521 tccccggtga ccagcaggtc ggcccccgtg gcgccggcct ggccgagctt gtccgccccc
     2581 atggccgtgg ccatctcggg gagccggacc gagaaggtcc ctccgaaccc gcagcaggcg
     2641 tccggccgtg ccatctccac cagctccagc ccttcgatgc cggccagcag gcggcgaccc
     2701 tgggacgcga gcccgagctc gcgaagcccg tggcaggcat cgtggaacgt gacccgcgcc
     2761 gggagcgagc cgatcctacc ctcgaccccc agctcgtcca ccaggaactg cgagagctcg
     2821 aaggtccgct cggccaggcc tcgggctcgc tcctggtggg gggtgccact gaacagcgtg
     2881 ggcaggttcg atcgcaccat cgcggcgcac gagcccgagg acgtgaccac cgtccccccg
     2941 tcaccgatgg ccccgatcag cgctccggcc atccgacggg cgtcgccggg gaacccggcg
     3001 ttgtacgcgg gttgtccgca gcaggtctgc cccccgggca cgtcgacctc cacgtccagg
     3061 tcgcggagca gtcgaaccgt ggcatcggcg atctccgggt acagcaggtc gatcaggcag
     3121 gtcacgaaca gcgagacgcg cgtgggacgc gtcccgccgg gcatccgctc acctcgaggc
     3181 gtcgcggacg acctcggcga agcggcggaa gagctcgcga ccgcgctcct cgtgcgcggg
     3241 catgtggcga gcgctctccc cccggatccg gtcgcccgat ttgccccagg tcgcttcgac
     3301 atccatctcg gcttccgcga tctccagcca ccatcccagc tccagggcgt cgagctcctg
     3361 atggaactgg accccccagg ctcgatcgcc cacccggaag gcctcgacgt cgatccggtc
     3421 acccgaggcc agcagggtcg cgccggccgg cagtcggtag gtgtcctcgt gccactggaa
     3481 caccatgtcc ccgtccacgt actgggacag gagggggtcc tccttcgcct ccccggtggg
     3541 ccggagcggc tcgaagccca cctccttcac cggcgcggcg aagacctctt cgccggccgc
     3601 tcgagcgagg agctgggccc ccaggcagat ccccaggtac gggacgcctc ggtccagcgc
     3661 ccggctcgtg tagtcgcggg cgccgcgcag gtacgggaac cggtcgacct ggtccacgtt
     3721 ggccgtgcct ccgaaggcga tcacgcccga gacctcgtcc agccggggca gcgaggcccc
     3781 ctcgatcagg ttggcggtac gcacgtccag gcccgcccag gccagggcgg tgggcgccac
     3841 gccgaacgtc tcgtaccgat cgttccgcat cagcaggacc ggttccatgg ttcgagtcta
     3901 gccgggcgcg gtgcggggcc ggatcgcccg cggcccaccg tcagctcccc gagcgctcca
     3961 gcacctgggg gttcagcggc gtgggagggc gccggccctc cagcaccgcc aacaggttgt
     4021 ccaccgccag catgcccatg gcgatcctgg tatccacgct ggcgcttccc aggtggggaa
     4081 cgatgaccgc gttctcgcag gccaccagat ccggatggac cgtgggctcc tcctcgaaga
     4141 cgtcgatccc ggcggcgaag atctccccgt cccggagggc ggccgccagg gcgacctcgt
     4201 cgacgatggg tccgcgagcc gagttcgcga gcaccgcggt ccccttcatg cgccggaact
     4261 cctcggttcc gaacaggtgc ctggtctgcg gcgtcagcgc ggtgttgatc gtgatgaagt
     4321 ccgcccgcgc caacagctcc gagaacccga cccgggtggc gccgaactcc cgctcagcct
     4381 ccggcgccgc caccacgtcg ctgtagatca cggtcatgcc gaagccgcgc ccgcgccgag
     4441 ccacggcctg cccgatccgg ccgaagccca ccacccccag cgtggcatcg tggacgtcca
     4501 caccgagcag catctgcggt ccccagatcc acggggtccc ggtccgaagg aagcgatccc
     4561 cttcggcgat gcggcgggcg gcggacagga gcagggccca ggtgaggtcg gccgtggtct
     4621 cggtcaggac gtcgggggtg ttggaggcga ccacgccgcg acgggtgcac tcgtcgacgt
     4681 cgatgttgtc gtagcccacc gcgtagttgg ccacgacctt caacgtcggc cccgcggcat
     4741 ccaggaaccc cgcgtcgacc ttctccgtga ggagcgtgat cacaccttgt cggcccggga
     4801 ccgcggccag cagctcgtcc cgggggatcg cgccctcgcc ccggtacacg tccaggtccg
     4861 agcgctcccg aagcacctcc atcacggggg gcggtaggag cctggtcacc aggaccgagg
     4921 ggcgttcagg cacgggacag ctccggcagc gaggcctgga acgcggccag ggcgatctcg
     4981 atgtcctcga gcgaggtcgc gtaggacagc cgcaggtagc catcgccgta ccgcccgaac
     5041 gcggtgcccg acagcgccgc gacccccgcc tcctccagca ggtgggcctc cacctccctg
     5101 gagctccgac ccagaccgct gacgttcggg aacacgtaga acgcgccatc cggggtgagg
     5161 caggagacgc cggggatcgc gttcagcccg tccacgatga cgtcgcgccg acggcggaac
     5221 tcctgcacca tccgctgtga gggttccatg ggaccggtca gggcggcgat cgcggcgtgc
     5281 tggctgaagg ccgaggtgca cgagaccgag ttgatggcga gccgagtcac gggctccacc
     5341 aggtactcgg ggaacacgcc gaaccccagc cgccacccgg tcatggccca cgtcttggac
     5401 cagccgtcca gcagcacggt gcgctcctgc atgccctcca cgtcgaggat gctggagtgc
     5461 tcgctctcgt accggatcat ccagtagacc tcgtccgaga gcaccacgat gtcgtgccgc
     5521 agcgcgatct ccgccaccgc ctcgaggtcc tgcttcgtga gcgcgctccc gcaggggttg
     5581 tggggggagt tcaggatcag cagcttcgtc cggtcggtga ccagggaggc cagctcctcc
     5641 gggtcgagcc ggaagccgtt ctgctcgcgg agcgggatcg ggacgggcgt ggcgccggag
     5701 aagttcgcga tcgactcgta catcgggaag ccggggtccg ggtagatgac ctcgtccccc
     5761 tcctcgcaca gggccaggat cgtgaagaac atgatgggct tggccccggg ggtcaccacc
     5821 acccgatccg gcgaggggga gaggcgtccg tgccgttcca ggaaggccgc cacggcctcg
     5881 cgcaggggcg ggatgccggg cgccgggacg taatgcgtca tcccccggtc gagagcttcc
     5941 ttggcggcgt cgcggatgtg ctccggcgtg gtgaagtccg gttcgccgag ctccagatgg
     6001 acgatctgcc gtcccttcgc ctccagggcc ttggcccggg ccaggacctc gaacgccgac
     6061 tccgtcccca gtcgccccat ccgggcagcg agttccatgg cagcctccac gctagagagg
     6121 gatcgttccg caggtcaagc taggaggtcc atcgccgagc ctacgggggc ggacgggccg
     6181 ccgcatcggg tccgaacggc cctgcgccta tcatccggtc ctccgagtac gggagggtgc
     6241 gcgatggcga gcgagctgat gatcctcggc ggggagcggg tccctgccgc cgatgggacc
     6301 acctacgagg tgatcgaacc ggggaccggt cggcccatgg cgcgggtctc ggaggccggg
     6361 accgaggacg cggccagcgc cgctcggatc gctcaccgtg ccttcgagga gggggcctgg
     6421 cgccggatgt ctggcaccca gcgcggccgg gtgctgctcc gagcggcatc gctcctgcgc
     6481 gagcgttccg agcagatggc caccgtcgag tcgaggaacg tcggcaaacc catcaggtgg
     6541 gcgaggggcg aggtggagac ggtggcgggg gtcctcgagt actggggcgg ggccgccaac
     6601 aagatcttcg gcgagacgat ccccgtgagc gctcccggtc tcgacctcac gctgcgggag
     6661 ccggtggggc cgtgtgtgct gatcaccccc tggaactacc cgctggtgat ctcctcgtgg
     6721 aagatcgggc ccgcgctcgc ctgtgggaac ccgatgatcg tgaagccggc cagcctcacg
     6781 ccgctgtccg tgctcctgct cgccgatgtc ctggtggagg cgggcttgcc tcccgagacg
     6841 atctcggtcc tccccggtcc gggcgctccc acgggggagg cgctggtgcg cgatcccctg
     6901 gtggcgaagg tcagcttcac gggctccacc gccgtcggtg tgcgggtcat gcagcacggg
     6961 gccgagcaca tcacgcgggt ctcgctcgag ctcggcggga aatcggcgaa cgtggtgttc
     7021 gccgacgccg acatggatcg atgcatcgag gagagcgtgt gggcggtgtt cgggaacacc
     7081 ggccaggatt gcacggcccg cacccggttg ttcgtggaac gccccgtgta cgaacggttc
     7141 gtggacgggc tcgcggcccg gacgcagcgg ctgcggatcg gacctcccac ggatgaggcc
     7201 accgaactgg ggccgatgat ctccgcggcc caacggcgga cgtcgctcga gtacctggag
     7261 gtcggccggg acgaaggggc ccgtctggtg atgggtggcg acgtgcccga ggagctggag
     7321 gagggcttct tcctccgccc ggcgatcctg gccgacgcct ccaacgacat gcgcgtggct
     7381 cgggaggaga tcttcgggcc ggtgg
//
LOCUS       DASQQQ010000006         4002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_275989, whole genome shotgun sequence.
ACCESSION   DASQQQ010000006 DASQQQ010000000
VERSION     DASQQQ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275989"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VE646_00280"
     CDS             complement(<1..580)
                     /locus_tag="VE646_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005264599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZD78468.1"
                     /translation="MAKLIYSAITSLDGYVADADGSFDWAAPDEEVHAFINDLERPIG
                     TYLYGRRMYEVMVFWETVALEDLPPFVRDYARIWRSADKVVYSRTLPGVSSSRTRLER
                     ELDPEELRRMKVSSARDLSVGGANLAGQTFADGLVDEVHLFLTPVVVGGGTQALPDLR
                     LDLELLELRRFGSGVVHLHYRTGSLTTRACGPS"
     gene            complement(596..1066)
                     /locus_tag="VE646_00285"
     CDS             complement(596..1066)
                     /locus_tag="VE646_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZD78469.1"
                     /translation="MPKLSAGLLLYRSAQEGLEVLLVHPGGPFWAKRDDGAWSVPKGE
                     YGADEDPLDAARREFREELGTDPPADPAPIPLGELRQPSGKRVTVWAVEGDVDVEAVH
                     SNTFTMTWPPGSDRTGEFPEVDRAGWFELGTARRKILSGQAGFLDRLSETLGAG"
     gene            1208..2224
                     /locus_tag="VE646_00290"
     CDS             1208..2224
                     /locus_tag="VE646_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015658438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZD78470.1"
                     /translation="MTAAAIEAEGLVKSFGPVRALDGIDLVATEGSVFGLLGPNGAGK
                     STAIRVLSTLLRPDAGRASVGGYDVIHRPAEVRRLIGLTGQYAAVDELLSGRENLYMI
                     GRLVGASAVEARRRAAELLESFDLADAGAKLVKTYSGGMRRRLDLAASLVGRPRFLYL
                     DEPTTGLDPRSRLELWSMIRTLVADGTTVLLTTQYLEEADRLADELVVIDHGRVIAAG
                     TPQQLKMRVGGQVLQAQPTDPADLAATERILAELAAGDERTYTDGQLVAVRIDDRSAM
                     GRAVRRLDEEGILVDDLSLRRPSLDEVFLALTGHLAEAEEDLLPESTDPDHRDDDVSG
                     RRSR"
     gene            2221..3009
                     /locus_tag="VE646_00295"
     CDS             2221..3009
                     /locus_tag="VE646_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020512039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD78471.1"
                     /translation="MSTATLSTPGREYVIRNTLTIARRNLMHIRSDPEQLVGMTIQPL
                     MFLVLFVYVFGGAIAGSSSRYLQFALPGILVQGIAFTPFTTALGLNVDFQRGVIDRFR
                     SLPIARSAVIGGRIAADAVRVIWGEVILIGFGLLLGFRFGGGGMGAVGAFVLVGLFGV
                     TLCWPMAFIGITARTPESVNTWGFLIILPLTFASSVFAPPETMPGWLEGFVKVNPITA
                     VVDATRGLMLGGPVADPVVRSLIWLVLIQAVFAPLAIARYRRRV"
     gene            complement(3045..3977)
                     /locus_tag="VE646_00300"
     CDS             complement(3045..3977)
                     /locus_tag="VE646_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZD78472.1"
                     /translation="MRIFVAGATGVVGRRLVPLLVQSGHEVIGSTRTPNKVGTIEAQG
                     ARGVLMDALDTEAVKEAVSEAAPEVVVHELTAIPAELDFRRFSEAFETTDRLRTEGTD
                     RLLAAASAAGVRRIVAQSYAAWPYEKRGGWVKTEDDPLDDRPPAGFRRTLEAIRHLES
                     AVLGAGMEGVVLRYGAFYGPGTSLAEGGPMLEPVRRRRFPIVGSGQGTWSFLHIDDAA
                     GATAIAVERGTGVYNITDDEPAAVRAWLPVLAEAIGARPPRRVPAWLARLLIGEAGVS
                     LMTQIRGASNAKAKRELGWKPVVPTWRQGFRTGL"
BASE COUNT          585 a         1496 c         1346 g          575 t
ORIGIN      
        1 gcgaaggccc gcaagcccgg gtagtcagag accccgtccg gtagtggagg tgcaccacgc
       61 cgctgccgaa ccgccgcagc tcgagcagct cgaggtccaa gcgtaggtcc ggcagcgcct
      121 gggtcccgcc gcccacgacc accggcgtca ggaacaggtg cacctcgtcc accaggccgt
      181 ctgcgaacgt ctggccggcc aggttcgcac cgcccacgct gaggtcccgc gccgaggaca
      241 ccttcatccg tcggagctcc tcgggatcga gctcccgctc cagccgcgtc cggctgctcg
      301 agacgccagg caacgtacgc gagtacacga ccttgtcggc gctccgccag atccgcgcgt
      361 agtcccgcac gaacgggggc aggtcctcca gggcgaccgt ctcccagaag accatcacct
      421 cgtacatccg ccgcccgtac aggtacgtgc cgatcggccg ctccaggtcg ttgatgaagg
      481 cgtggacctc ctcatccggc gccgcccagt cgaacgagcc gtccgcatcg gccacgtacc
      541 cgtcgagcga ggtgatcgcc gagtagatca gcttggccac ggttacaccc ccacctcagc
      601 ccgcccctaa cgtctccgac agccggtcca ggaagccggc ctgacccgac aggatcttcc
      661 gccgggcggt gcccagctcg aaccaccccg cccggtccac ctccgggaac tcgccggtgc
      721 ggtccgaccc cggcggccag gtcatcgtga acgtgttgct gtgcacggcc tccacatcca
      781 cgtcaccctc cacggcccag accgtcaccc gcttgccgct cggttgccgg agctccccca
      841 gggggatcgg cgcggggtcg gctggcgggt cggtgcccaa ctcctcccgg aactcccggc
      901 gggcggcgtc caagggatcc tcgtcggccc cgtactcacc cttgggaacc gaccaggcgc
      961 cgtcgtcacg cttggcccag aagggccctc ccggatgcac cagcaagacc tccaagccct
     1021 cctgcgccga gcggtacagg agcaggcccg cgctgagctt cggcataccc agcctccgat
     1081 ctcggttcag cctcccaagg atgaccgacc gccgacgggg gggcgaaccg gggcggggac
     1141 cgatgagtcc acgggcccat ccgggtcgga aggaccgacc ccatcgacga accgggagga
     1201 gggagacatg accgctgcgg cgatcgaggc cgagggcctc gtgaagagct tcgggccggt
     1261 ccgggcgctc gacggaatcg acctggtggc caccgagggc agcgtcttcg ggctgctcgg
     1321 accgaacggt gccggcaagt ccaccgcgat ccgggtgctg tccacgctgc tgcgacccga
     1381 cgcgggccgg gcgtccgtgg gcggttacga cgtgatccat cggccggccg aggtgcgccg
     1441 gttgatcggg ctgaccggcc agtacgcggc cgtggacgag ctgctgtccg gccgggagaa
     1501 cctctacatg atcggccgtc tggtgggggc ctcggccgtc gaggcgcggc gccgggccgc
     1561 cgagctcctg gagtcgttcg acctggccga cgcgggcgcg aagctcgtga agacctattc
     1621 gggggggatg cgccgccggc tggacctggc cgcgagcctg gtcggacgtc cgcgcttcct
     1681 gtacctggac gaacccacca cgggcctgga tccccggagc cgcctggagc tctggtcgat
     1741 gatccgcacg ctggtggccg acggcacgac cgtgctgctc accacgcagt acctggagga
     1801 ggccgaccgg ctggcggacg aactcgtggt gatcgaccac ggacgcgtga tcgcggccgg
     1861 cacgccgcag cagttgaaga tgcgagtggg cggccaggtc ctgcaggcgc agccgaccga
     1921 ccccgccgac ctggccgcga ccgagcgcat cctggccgag ctcgccgcgg gggacgagcg
     1981 cacgtacacg gacggtcagc tcgtggccgt gcggatcgac gaccggtcgg cgatgggccg
     2041 ggcggtccga cgcctggacg aggaggggat cctggtggac gacctctcgc tccggcggcc
     2101 gagcctggac gaggtcttcc tggccctgac cggccacctg gcggaagccg aggaggacct
     2161 cctacccgag agcaccgacc ccgatcaccg agacgacgac gtctccggaa ggaggagccg
     2221 atgagcacag ccaccctcag cacgcccggc cgcgagtacg tgatccgcaa cacgctcacg
     2281 atcgcccggc ggaacctgat gcacatccgg tccgaccccg agcagctcgt cggcatgacg
     2341 atccaaccct tgatgttcct ggtgctgttc gtctacgtgt tcggtggtgc catcgcgggg
     2401 tccagcagcc gctacctgca gttcgccctg ccggggatcc tggtccaggg catcgccttc
     2461 acgccgttca ccacggccct ggggctgaac gtggatttcc agcgcggagt catcgaccgc
     2521 ttccgctccc tgcccatcgc gagatcggcc gtgatcggcg gccggatcgc cgccgatgcc
     2581 gtccgggtga tctggggcga ggtgatcctg atcggcttcg ggctgctgct gggattcagg
     2641 ttcgggggcg gggggatggg tgccgtgggg gcgttcgtgc tggtcggact cttcggcgtg
     2701 acgctgtgct ggccgatggc cttcatcggc atcaccgcca ggacgcccga atccgtcaac
     2761 acctggggct tcctgatcat cctgccgctc acgttcgcga gctccgtgtt cgccccgccg
     2821 gagacgatgc cggggtggct ggaaggcttc gtgaaggtga accccatcac cgccgtggtg
     2881 gatgccaccc ggggcctgat gctcggcggg ccggtggcgg accccgtggt ccggtcgctg
     2941 atctggctgg tgctgatcca ggccgtcttc gcgccgctgg ccatcgcccg ctaccgccgg
     3001 cgggtctaga acgacccgcc ggcgcccctg gcccgaggtc atcctcaaag cccggtgcgg
     3061 aagccctggc gccaggtcgg gacgaccggc ttccacccca gctcccgctt cgccttggcg
     3121 ttcgaggcgc cccggatctg cgtcatcaac gagaccccgg cctcaccgat cagcagccgc
     3181 gccagccacg cggggacacg gcgcggaggc ctggccccga tcgcctcggc cagcaccggc
     3241 agccatgcgc ggaccgccgc tggctcgtcg tcggtgatgt tgtagacgcc cgtcccccgc
     3301 tccaccgcga tcgccgtcgc ccccgcggca tcgtcgatat gcaggaacga ccaggtgccc
     3361 tggccggacc ccacgatcgg gaaccgtcgg cgacgcaccg gctcgagcat cggcccgccc
     3421 tcggccagcg acgtccccgg cccgtagaac gcgccgtacc ggagcaccac cccctccatc
     3481 ccggcgccca gcaccgccga ctcgagatgg cggatcgcct cgagcgtgcg ccggaacccg
     3541 gcaggcgggc gatcgtccag gggatcgtcc tccgtcttca cccagccccc gcgcttctcg
     3601 tagggccagg ccgcgtagct ctgggccacg atccgtcgaa cgccggcggc ggaggccgcc
     3661 gccagcaggc ggtcggtccc ttcggtccgg agccggtcgg tggtctcgaa ggcctcggag
     3721 aaccggcgga agtcgagctc cgctgggatg gcggtcagct cgtgcaccac cacctcgggc
     3781 gccgcctcgg acacggcctc cttcaccgcc tcagtgtcga gggcgtccat cagcacgcca
     3841 cgcgcaccct gggcctcgat cgttcccacc ttgttcggcg tgcgggtcga tccgatcacc
     3901 tcgtggccac tctgcaccag cagcggcacc aggcgccgac ccaccacacc ggtcgctccc
     3961 gcgacgaaga tcctcatcgc caactccttc ctcggttcct ac
//
LOCUS       DASQQQ010000007         4607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_276076, whole genome shotgun sequence.
ACCESSION   DASQQQ010000007 DASQQQ010000000
VERSION     DASQQQ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276076"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="VE646_00305"
     CDS             complement(<1..1134)
                     /locus_tag="VE646_00305"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HZD78473.1"
                     /translation="MQEGFTPEERELLSNHFTNLDGPVFALVNLPEVVKGALFARYSR
                     TTKSLRRLFLDEFAKDVHEVGVTGHVGTGRAERLYDNVFFEYGDDSVAQLGGVHLACE
                     QASQLLAKAIEWGRLAAYLEQSTRYMRYDDRPGGRWRLTVPPEIEGTSAEQAYQDFAT
                     RTFEAYGRMFQPLYEHFAARFPKDPADSDFIYRSTITAKTCDTLRVLLPAGTRSNLGA
                     YADGQAFEMMLLRLRAHPLAEVREYADLMLTELRKVIPSFLKRVDLPDRGVAWSEYQR
                     RTREATAEVAAKLTARVDAPTGGSEVTLTDWEPDGEEKIVAAALYAATDLPDAELREA
                     AHGLSDAERTEVLRAYVGERGNRRHKPGRAFERTGYRFDVLCDY"
     gene            complement(1146..2891)
                     /gene="pknB"
                     /locus_tag="VE646_00310"
     CDS             complement(1146..2891)
                     /gene="pknB"
                     /locus_tag="VE646_00310"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HZD78474.1"
                     /translation="MSDGPKRVRTPVLLAGRYRVEEELGRGGMAKVFKGTDTTLGRPV
                     AVKVLAPQFAEDANFVSRFRREAQAAARLNHPNLVGVYDTGSDDGVHYIVMEFVDAKT
                     LADYLAGGGRIMPERAVELAEDVCRALEVAHAHGVIHRDIKPANIMVTPRGDVKVTDF
                     GIARVISGADTVAQTAAVLGTASYLSPEQARGEPVDQRTDIYSLGVVLYEMVTGRPPF
                     SGDTPVAVASKHVLEQPVTPSSINADVPPALDAVIMKALAKNPANRYRSAEEFRQDLE
                     RVRLGQPVGATPLLPQEAPTQVIAPSAAAPVEGAGRTSVLPPQPPAPEPPRRRWWVGV
                     VVGLLILAALGAGAYILASGLLGGGEPGATTFAVPNVFGDKLPDATAKLQEAGFKVRQ
                     PVIRQEDPNHTPGTVIKQDPAADTQMPKGTEITLTVAKAPPVQIPTGLIGHPADEVKA
                     TLEGLGLTVDPQEQSSTDVEKDHVISLDPGEGQTIKAGGTVTMVVSTGPGQVHVPDLV
                     CQPLGKAQTDLTKEGLDSLVAQGTQFNAECPQPGKVAATEPGAGTPVEAGTVITLIES
                     TNVSPTPSPSESPTP"
     gene            complement(2891..4384)
                     /locus_tag="VE646_00315"
     CDS             complement(2891..4384)
                     /locus_tag="VE646_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZD78475.1"
                     /translation="MDRQVRRLGLALLGLFVVLFAQVNYLQVFGAQRLEDNPANARRQ
                     LIAEYEVDRGDILAADGQTVLATSRKSSGELKYQRRYPSGPLYAGITGYYSLVYGRSE
                     LEQAQNTFLSGDAPQLLPQTLADQILGRPKRGATVVTTIDPVVQLAAAKALGDLPGGV
                     VAMDPRTGEVKAIVANPTYDPNRLSSPDRTKVLRAWKQLTDAPDHPLVSRAIDDVFPP
                     GSTFKLVTAAAALQNGYGPDSQWDNPRVLDLPQTSATLENFGGEHCLGGAPKITLAQA
                     LQVSCNVVFGEIGLELGADKLAAQARAFGFAPDATSGDIPFDIPFQEGVFPDPSYFSD
                     RLPAVALSAIGQDNVAANPLQMALVASAVANGGVEMRPRLVSEIRDPSGRILERYQPE
                     VWGRPLSAASAAELTQMMVNVVRAGTGTAAQIPGVDVAGKTGTAQHGKGLAPHAWFVS
                     FAPSQDPQIVVAVLVLDGGSLGSDATGGRVAAPIAKAVMEAALNEGS"
     gene            complement(4386..>4607)
                     /locus_tag="VE646_00320"
     CDS             complement(4386..>4607)
                     /locus_tag="VE646_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005175894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HZD78476.1"
                     /translation="DAFGKLLATGLAAVIGLQTFVIVGGVVRVIPLTGVTLPFVSYGG
                     SSLVANFILLALLIRVSAGPAPPRHRRAT"
BASE COUNT          640 a         1636 c         1620 g          711 t
ORIGIN      
        1 gtagtcgcac agcacgtcga accggtagcc ggtgcgctcg aaggcccgac ctggcttgtg
       61 tcgccggttc ccacgctcgc cgacgtaagc gcgcaggacc tcggtccgct ccgcgtccga
      121 gaggccatgc gccgcctcgc ggagctcggc gtcgggcagg tcggtggccg cgtagagggc
      181 ggccgcgacg atcttctcct cgccgtccgg ctcccagtcc gtgagggtga cctcggaccc
      241 gccggtggga gcgtccaccc gggcggtgag cttggccgcc acctcggcgg tggcctcacg
      301 ggtccggcgc tggtactccg accaggccac gccgcggtcg ggcaggtcga cccgcttcag
      361 gaacgagggg atcaccttgc ggagctcggt cagcatgagg tcggcgtact cgcgcacctc
      421 ggccagcggg tgggctcgga ggcggaggag catcatctcg aaggcctggc cgtcggcgta
      481 cgcgcccagg ttcgagcgcg tgcccgccgg cagcagcacg cggagcgtgt cgcaggtctt
      541 cgcggtgatc gtggagcggt agatgaagtc cgagtccgcc gggtccttgg ggaagcgggc
      601 cgcgaaatgc tcgtacaggg gctggaacat ccgcccgtag gcctcgaagg tgcgggtggc
      661 gaagtcctgg taggcctgct cggctgacgt gccctcgatc tcgggcggca cggtgaggcg
      721 ccaccggccc cccggccggt cgtcgtagcg catgtagcgg gtggactgct cgaggtaggc
      781 ggcgaggcgg ccccactcga tggctttggc caagagctgg gaggcctgct cgcaggcgag
      841 atgcacgccg ccgagctgcg ccaccgagtc atcgccgtac tcgaagaaca cgttgtcgta
      901 caggcgctcg gcccgcccgg tgccgacgtg cccggtcacg cccacctcgt gcacgtcctt
      961 cgcgaactcg tccaggaaca gccgccggag cgacttggtg gtccgcgagt accgggcgaa
     1021 cagcgcgccc ttcaccacct ccggcagatt gaccagcgcg aacaccggcc cgtccaggtt
     1081 cgtgaagtgg ttcgagagga gctcgcgctc ctcgggggtg aatccctcct gcacgtacca
     1141 catcgtcatg gcgtggggct ctccgagggg ctcggagtgg gcgagacgtt cgtggactcg
     1201 atcaaagtga tcacggtacc cgcttctacc ggcgtgcccg cgccgggctc cgtggccgcg
     1261 accttgcccg gctgtgggca ctccgcgttg aactgtgtcc cctgggccac cagcgagtcc
     1321 agcccctcct tggtcagatc cgtctgagcc ttgccgagcg gctggcagac gaggtctggc
     1381 acatggacct gcccgggtcc ggtcgagacg accatcgtga cggtgccgcc ggccttgatc
     1441 gtctgtccct ctccggggtc caggctgatc acgtgatcct tctccacgtc cgtgctgctc
     1501 tgctcctgtg gatccaccgt gagtcccaag ccctccagcg tggccttcac ctcgtccgca
     1561 ggatgaccga tcagtccggt ggggatctgg accggcggtg ccttggccac ggtcagcgtg
     1621 atctcggtgc ccttgggcat ctgggtgtcc gccgccggat cctgcttgat cacggtcccg
     1681 ggggtgtggt tcgggtcctc ctgacggatc acgggctgcc tcaccttgaa cccggcctcc
     1741 tggagcttcg cggtagcgtc cggcagcttg tccccgaaga cgttcggtac ggcgaaggtg
     1801 gtagcgcccg gttccccgcc gcccaacagg ccgctggcca ggatgtacgc tcccgcaccg
     1861 agcgcggcca ggatcagcag ccccaccacg acgccgaccc accagcggcg ccggggtggc
     1921 tcgggagccg ggggctgggg cggcagcacc gaggtgcgcc ccgcaccttc cacgggagcc
     1981 gcggccgaag gagcgatcac ctgggtgggc gcctcctgtg gcagcagcgg cgtggcgccc
     2041 accggctggc ccagccggac ccgctccagg tcctgacgga actcctcggc cgatcggtac
     2101 cggttcgccg ggttcttcgc cagcgccttc atgatcacgg cgtccagcgc cggcgggacg
     2161 tcggcgttga tgctggaggg cgtgaccggc tgctcgagca cgtgcttcga cgccaccgcc
     2221 accggggtgt cgcccgagaa cggcggccga ccggtcacca tctcgtagag caccacgccc
     2281 agcgagtaga tgtcggtgcg ctggtccacc ggctcgccgc gcgcctgctc gggcgacagg
     2341 taggaggccg ttcccaggac cgcggcggtc tgggccaccg tgtcggcgcc cgagatcacc
     2401 ctcgcgatcc cgaagtccgt gaccttcacg tcgccccgag gcgtgaccat gatgttggcg
     2461 ggcttgatgt cccgatggat cacgccgtgg gcgtgcgcca cctcgagggc ccggcagacg
     2521 tcctcggcaa gctcgacggc ccgctccggc atgatccggc ccccgccggc caggtagtcg
     2581 gccagggtct tggcgtccac gaactccatc acgatgtagt ggacgccgtc gtccgagccg
     2641 gtgtcgtaca cgccgaccag gttcggatgg ttcaggcggg cggcggcctg ggcctcgcgc
     2701 cggaaccggg agacgaagtt cgcgtcctcg gcgaactggg gcgcgaggac cttgacggct
     2761 accggccggc ccagggtggt gtcggtcccc ttgaagacct tggccatgcc gccgcgcccg
     2821 agctcctcct ccacccggta ccgaccggcc aggaggaccg gggtccgtac gcgcttcggt
     2881 ccgtcgctca tcacgatccc tcgttcaggg cggcctccat gaccgccttg gcgatggggg
     2941 cggccacccg gccccccgtc gcgtcgctgc caaggctacc gccgtcgagc accaggacgg
     3001 ccaccacgat ctgcggatcc tgtgacggcg cgaaggacac gaaccaggca tggggggcca
     3061 ggcccttgcc gtgctgggcg gtcccggtct tgcccgccac gtcgaccccc ggtatctggg
     3121 ccgcggtccc ggtcccggcc cgcaccacgt tcaccatcat ctgcgtgagc tcggcggccg
     3181 aggctgccga cagcggtcgt ccccacacct ccggctggta gcgctcgagg atccggccgc
     3241 tcggatcccg gatctcgctc accaggcggg gccgcatctc cactccgccg ttcgcgacgg
     3301 ccgaggccac cagggccatc tggagcgggt tcgccgccac gttgtcctgg ccgatcgccg
     3361 agagcgcgac ggcgggcaga cggtccgaga agtacgaggg gtcggggaac acgccttcct
     3421 ggaacgggat gtcgaagggg atgtcgccgc tggtggcgtc gggagcgaac ccgaacgctc
     3481 gggcctgcgc ggcgagcttg tccgctccga gctccagccc gatctcgccg aacaccacgt
     3541 tgcacgagac ctgcagcgcc tgggccagcg tgatcttggg agccccgccc aggcagtgct
     3601 cgccgccgaa gttctcgagc gtggccgagg tctgaggcag gtccagcacc cgagggttgt
     3661 cccactggct gtccggtccg tacccgttct gcagcgcggc ggcggccgtg accagcttga
     3721 aggtggaccc gggcgggaac acgtcgtcga tcgcccgaga gaccagcggg tggtcggggg
     3781 catcggtcaa ctgcttccag gcccggagga ccttcgttcg gtccggggag gacaggcggt
     3841 tcggatcgta cgtcgggttg gccacgatgg ccttcacctc accggtcctc gggtccatgg
     3901 cgaccacgcc gcccgggagg tccccgaggg ccttggccgc cgccaactgc acgacggggt
     3961 cgatcgtcgt gaccaccgtg gccccccgct tgggacggcc caggatctga tccgcgagcg
     4021 tctgcgggag cagctgcggc gcgtccccgg agaggaaggt gttctgggcc tgctccagtt
     4081 cgctccgccc gtagacgagc gagtagtagc cggtgatccc ggcgtacagg ggcccgctcg
     4141 ggtaccggcg ctggtacttc agctctccgg agctcttccg gctggtcgcc agcaccgtct
     4201 gtccatcggc ggccaagatg tcgccgcggt ccacctcgta ctcggcgatg agctggcgac
     4261 gggcgttcgc cgggttgtcc tccaggcgct gtgccccgaa cacctgcagg tagttgacct
     4321 gcgcgaagag gaccacgaac agcccgagca gggccaggcc gaggcggcgg acctgccggt
     4381 ccatgctagg tagccctccg gtgccggggc ggcgctggcc ccgccgagac ccggatcagc
     4441 agcgcgagca agatgaagtt cgccaccagg ctcgaccccc cgtagctcac gaacggcagc
     4501 gtcacccccg tgagcgggat cacccggacc acgcccccca cgatcacgaa ggtctgcagg
     4561 ccgatcaccg ccgccaggcc ggtggcaagc agcttcccga aggcatc
//
LOCUS       DASQQQ010000008         4520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_27889, whole genome shotgun sequence.
ACCESSION   DASQQQ010000008 DASQQQ010000000
VERSION     DASQQQ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27889"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..200)
                     /locus_tag="VE646_00325"
     tRNA            complement(126..200)
                     /locus_tag="VE646_00325"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(166..168),aa:Val,seq:gac)
     gene            complement(232..302)
                     /locus_tag="VE646_00330"
     tRNA            complement(232..302)
                     /locus_tag="VE646_00330"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(268..270),aa:Cys,seq:gca)
     gene            complement(351..1175)
                     /locus_tag="VE646_00335"
     CDS             complement(351..1175)
                     /locus_tag="VE646_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD78477.1"
                     /translation="MEDAIKVGLARGRAVGIDGLSLALDAAGDVTIVGDISLESGGAR
                     AMAAAEADVVLVDSGTDPSSARGMTRRIVRANPSARVMVLGCSEDQRDLEAAVRAGAR
                     GYVVKPAGPEDLVGALRLVASGHLVIDRRFADVALPRAEPEPARPRGRDLALTEHNVR
                     VLMLAVSGQSDEQIASILGVSLGTVRRHLGTIRATFGAPDRRSAIAHARQLRYLLVRG
                     KGTYEIWDTARPDAPLDRFEGSQRTQAWLLWRDLTAGRQRGGPWRRFQAWIRSRRA"
     gene            1429..1623
                     /locus_tag="VE646_00340"
     CDS             1429..1623
                     /locus_tag="VE646_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78478.1"
                     /translation="MKKQEAWFYGPRGEIRPSQVGHTRRSFCPRCGALLWPTEGSGDP
                     APLCLDEVLRSRPASGMQAA"
     gene            complement(1645..2010)
                     /locus_tag="VE646_00345"
     CDS             complement(1645..2010)
                     /locus_tag="VE646_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HZD78479.1"
                     /translation="MNLIGSRADRWWNDPDRAVRDLIEELGRFSSAEEEITVVFDRRP
                     ADLQSGRRAGVEVAFASRRGRNAADREIVELVAADADPASLTVVTSDRRLGERVRELG
                     ARVTGSGAFRRRLDQEDLR"
     gene            complement(2044..2526)
                     /locus_tag="VE646_00350"
     CDS             complement(2044..2526)
                     /locus_tag="VE646_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78480.1"
                     /translation="MRSGDIAQAVGKGLIAGAAGTMALTVSQLMAEKLFHQPMPTASA
                     DAAEKLVGVEPKDASGKFRLGMLTHFAYGSLWGVPRALMERSGFRGWRATAFHAAAVQ
                     TTAGAVLPALDVGPPPTKMPGKTLGLNTLHHSVYALVTGAVVRLLDRRSRLGRLAPAS
                     "
     gene            2631..3056
                     /locus_tag="VE646_00355"
     CDS             2631..3056
                     /locus_tag="VE646_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016333572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HZD78481.1"
                     /translation="MLRTLLIALAAAGVWLIAVMILLAAGRRTAARELATLLPNLLLL
                     FRGIVRDPRCPRGSKLLLLFALAWIASPVDLVPEFIPLLGPLDDAVVAALVLRHVIRR
                     AGPAIVREHWRGDPTTVQRLLRLSGVDPSIRGGPGPTPS"
     gene            complement(3025..3369)
                     /locus_tag="VE646_00360"
     CDS             complement(3025..3369)
                     /locus_tag="VE646_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78482.1"
                     /translation="MGNEERPDVIELWEEENGRWLWRYRSADRYSLTSAEDFVERDEA
                     LDTAEISYPGVPIVQIPGPAVRRRRNRRVLWTAGVLALWLLVKALRKLLRLIRTVRVA
                     GRVMKASGRARP"
     gene            complement(3359..>4520)
                     /locus_tag="VE646_00365"
     CDS             complement(3359..>4520)
                     /locus_tag="VE646_00365"
                     /inference="COORDINATES: protein motif:HMM:NF025016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HZD78483.1"
                     /translation="YDVRGGLVLRQMHHWAALLFLAAIIVHMCRIFFTGAFRKPRDIN
                     WLVGVTLLLLSMGTAFLGYVLPDDLISGNGLRIAYAILISIPVIGPWLVFLLFGPNYP
                     GTDIMHRFYILHVFVIPVVLLGLLAVHLMLIWRQKHTQFPGPGRTETNVVGSRVWPAY
                     SMRSTGLFLGALAVVGILGGVAQIDPIWLFGHYDPTTISAPAQPDWYLAWVEGALRIY
                     PRWAERSMWTIPVQFFPAVLMPLLTFAGLYAWPWIERAVTGDRLSHQLLDRPRYAPGR
                     TAVGAALLSFYIMLTIDATNDLWAHYFHLSVLAVTWGLRIATLVVPFIVGPVVHQLMR
                     ALGRSDADHFIKMPLEPLSHQMQRWKPSRVADRMAGDGGRSSVEEEEPTHGE"
BASE COUNT          692 a         1654 c         1516 g          658 t
ORIGIN      
        1 cgtgaacgat gacggcgagc tcctccgcga gctcgtgctc gatcccaccc gggactacca
       61 gcccataggc tgaacgcgga acgatgtcgc gggacacgtg cgaacgatgt cccgagacat
      121 cacactggtg ggcgctagag gatttgaacc tctgacctct tccgcgtcaa ggaagcgctc
      181 tacccctgag ctaagcgccc gagggggtca tggtacccga cggcaccagc gaggcgggga
      241 ccggaattga accggtgtac agggttttgc agacccttgc ctaaccactc ggccaccccg
      301 ccggcgatcc ggggcttccg gaccggtagc tagtctacgt gaagggccgc tcaggcccgg
      361 cgggatcgga tccacgcttg gaaccggcgc cagggcccgc cccgctgtcg gcccgccgtg
      421 agatcgcgcc acagcagcca ggcctgggtc cgctggctcc cctcgaaccg gtccagcggg
      481 gcatccggtc tggccgtgtc ccagatctcg taggttccct tgccccggac cagcaggtag
      541 cggagctgcc tggcgtgtgc gatcgcgctc cgccggtccg gtgccccaaa ggtggcccgg
      601 atcgtcccca gatgtcggcg caccgttccc agggagaccc cgaggatgga cgcgatctgc
      661 tcgtcgctct ggcccgagac cgcgagcatc aacacccgga cgttgtgttc ggtcagggcg
      721 aggtcgcgcc ctcgagggcg tgcgggttcc ggttcggccc tcggcagggc gacgtcggcg
      781 aaccgccgat cgatcaccag gtgcccggag gcgaccaggc gaagcgcccc taccaggtcc
      841 tcgggcccgg ccggcttgac cacgtagccg cgcgcccccg cccggacggc cgcctccagg
      901 tcccgctgat cctccgaaca gcccaggacc atgacccggg ccgaggggtt cgctcgcacg
      961 atccgcctgg tcatgccccg ggcgctggag gggtcggttc cgctgtccac caggaccacg
     1021 tcggcctccg cggccgccat cgcccgggct ccaccgctct ccagggagat gtcgccgacg
     1081 atggtcacgt cgcccgctgc atcgagcgcg aggctcaggc catcgatccc caccgctcgg
     1141 ccccgcgcca ggccgacctt gatcgcgtcc tccacggtcc ccttcgcccc cagatcggtc
     1201 cctccccact gtgacgggtg aggctcggtg tcggcatcgg ccggaaggcg gttcctggct
     1261 cccccgttca gcggcagccc cggggtgcta ggcccaacgg cgcgatgcct tgccgggcaa
     1321 cgcttcgagg gccttgcccg atgtttgccg taggaccgct tcgggaacgg ccacagcggg
     1381 tatcggcacg tcggctgagg cacgagggct gccggggagg gtcgagagat gaagaagcag
     1441 gaagcctggt tctacggacc gcggggggag atccgcccct cccaggtcgg tcacacccgc
     1501 cgttcgttct gcccgcgatg cggcgcgctc ctgtggccga cggagggttc gggcgatccg
     1561 gcacccctct gcctggacga ggtgctccga tcccgtccag cctccggcat gcaggcggcc
     1621 tgagcggtct cgagggccac cgcgctagcg gaggtcctcc tggtccaggc gacgccggaa
     1681 cgcgccggat ccagtgacgc gggcgccgag ctcgcggacc cgctccccca gccgccgatc
     1741 cgaggtcacc acggtgagcg aggccggatc cgcatcggcg gcgaccagct ccacgatctc
     1801 gcggtccgcg gcgttccggc ctcgccgcga ggcgaacgcg acctccacgc cggcccgccg
     1861 gccggactgc aggtcggcgg gccggcggtc gaagacgacg gtgatctcct cctccgcgct
     1921 tgagaaccgc cccagctcct cgatcaggtc acggaccgcg cggtcggggt cgttccacca
     1981 ccgatcggcc cgggagccga tcaggttcat gccgtcgatc agccaccgac gggtcgccac
     2041 tgctcaagag gcgggtgcga gccgacccag acggctgcgc cggtccagga gacggacgac
     2101 cgctcccgtt accagcgcgt agacgctgtg gtgcagcgtg ttcaggccca aggtcttgcc
     2161 cggcatcttg gtgggtggcg ggcccacgtc caacgcgggc aggacggcgc cggccgtggt
     2221 ctgcacggcg gccgcgtgga aggccgtggc gcgccatccc cggaagccgc tccgctccat
     2281 gagcgcccgg ggcacgcccc agagcgagcc gtaggcgaag tgcgtcagca tgcccagacg
     2341 gaacttgccc gacgcgtcct tgggctccac ccccacgagc ttctccgccg catcggccga
     2401 cgccgtcggc atcggctgat ggaagagctt ctcggccatc aactgtgaga cggtcagggc
     2461 catggtcccg gccgcgcccg cgatcagacc cttccccacg gcttgtgcga tgtctccgga
     2521 acgcatgcgt gtcctccttc ctaccttccg atgcttcccg gcgcggccag gccccatacc
     2581 tggccgaagc cggttggccc gaccggtgtc ccgtggacga tgatggtgag gtgctccgaa
     2641 ccctgctcat cgcactggcg gcggcgggcg tctggctgat cgcggtgatg atcctcctcg
     2701 cggccggtcg ccggaccgcc gcgcgtgagc tcgccaccct cctgccgaac ctgctgctgc
     2761 tgttccgggg gatcgtccgc gacccccggt gccctcgggg ctccaagctc ctgctcctgt
     2821 tcgccctggc gtggatcgcc tcgccggtgg acctggtccc ggagttcatc ccgctcctgg
     2881 gcccgctgga cgatgccgtg gtcgccgccc tggtcctgcg tcacgtgatc cggcgcgcgg
     2941 gtccggcgat cgtgcgcgaa cactggcgag gcgatccgac caccgtgcag cgactcctgc
     3001 gcctgtcggg cgttgacccc tcgatcaggg gcgggcccgg cccgacgcct tcatgacgcg
     3061 gccggcgacc cggacggtgc gaatcagccg gagcagcttc cggagcgcct tcaccaacag
     3121 ccacagggcg agaaccccgg cggtccagag gacccgacgg ttccgtctgc gccgcaccgc
     3181 cggccccggg atctgaacga tcgggacccc agggtacgag atctccgcgg tgtccagggc
     3241 ttcatcccgc tcgacgaagt cctccgcgct ggtcagcgag tagcggtcgg cgctccggta
     3301 gcgccacagc cacctgccgt tctcctcctc ccacagctcg atcacgtcag ggcgctcctc
     3361 attccccatg ggtcggctcc tcctcctcga ccgacgaacg cccgccgtcc ccagccatcc
     3421 ggtccgccac ccgggacggc ttccatcgct gcatctgatg ggacagcggc tcgaggggca
     3481 tcttgatgaa gtgatccgcg tcgctccggc ccagtgcccg catcagctga tgcacgacgg
     3541 gccccacgat gaacgggacc accagggtgg cgatccgcag tccccaggtc accgccagca
     3601 cggaaaggtg gaaatagtgc gcccacagat cgttcgtggc gtcgatcgtg agcatgatgt
     3661 agaagctcag cagcgcggcc cccaccgccg tccgacccgg tgcgtagcgg ggccgatcca
     3721 gcagctgatg cgacagacga tcaccggtca cagcccgctc gatccagggc caggcgtaca
     3781 gcccggcgaa cgtgagcagc ggcatcagga ccgcggggaa gaactgcacc gggatggtcc
     3841 acatcgagcg ctcggcccag cgcgggtaga tgcgcagcgc gccctcgacc caggccaggt
     3901 accagtcggg ctgggcgggc gcgctgatcg tggtggggtc gtagtgcccg aacaaccaga
     3961 tcgggtcgat ctgcgccacg ccacccagga tccccacgac cgccaaggct cccaggaaca
     4021 gccccgtgga gcgcatcgag taggccggcc acacccgcga gcccacgacg ttggtctcgg
     4081 tgcgtcccgg gcccgggaac tgcgtgtgct tctgtcgcca gatcagcatc aggtggaccg
     4141 ccaggagtcc cagcaggacc accgggatca cgaacacgtg gaggatgtag aagcggtgca
     4201 tgatgtcggt ccccgggtag ttggggccga agagcaggaa caccaaccag ggtccgatca
     4261 ccgggatcga gatcaggatc gcgtaggcga tccgcagccc gttgcccgag atcaggtcgt
     4321 ccggcagcac gtacccgagg aacgccgtcc ccatcgagag cagcagcagg gtcacgccca
     4381 ccagccaatt gatgtcccgg ggcttgcgga acgcgccggt gaagaagatc cggcacatgt
     4441 gcacgatgat cgcggccagg aacagcagcg ccgcccagtg gtgcatctgc cgcagtacca
     4501 ggccaccccg tacgtcgtac
//
LOCUS       DASQQQ010000009        10053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_138177, whole genome shotgun sequence.
ACCESSION   DASQQQ010000009 DASQQQ010000000
VERSION     DASQQQ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10053
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138177"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..460)
                     /gene="ssrA"
                     /locus_tag="VE646_00370"
     tmRNA           complement(106..460)
                     /gene="ssrA"
                     /locus_tag="VE646_00370"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(474..899)
                     /locus_tag="VE646_00375"
     CDS             complement(474..899)
                     /locus_tag="VE646_00375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZD78484.1"
                     /translation="MIRDARPEELDAVLRVWWEAGADESPTDDPVGLARLPVRDPHAL
                     LVADADGRLVGTLVVGWDGWRGSMYRLAVVPSMRRRGIATALVAEGERRLRELGARRI
                     SALVISSEEAAAGFWREVGYEHDARMHRYARSLPPLSYT"
     gene            complement(905..1381)
                     /gene="smpB"
                     /locus_tag="VE646_00380"
     CDS             complement(905..1381)
                     /gene="smpB"
                     /locus_tag="VE646_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017567748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HZD78485.1"
                     /translation="MSPSRSSAPGQKTVVSNRRALHDYEVLERCEAGMALTGSEVKSL
                     RGGRGSLAEAYARIRDGEVWLEGMHIPPYQQGDKRTYDPVRIRKLLLRRDQIERLVGK
                     TAERGLALVPLRVYFAHGLAKLELALARGKRQYEKRQTIAEREHKREMEQAARRRR"
     gene            complement(1403..2284)
                     /gene="ftsX"
                     /locus_tag="VE646_00385"
     CDS             complement(1403..2284)
                     /gene="ftsX"
                     /locus_tag="VE646_00385"
                     /inference="COORDINATES: protein motif:HMM:NF038346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HZD78486.1"
                     /translation="MTGRRFDYLFREVSLGLRRNGLVAFAAVSTTFIALLLFGLAMLI
                     TRQVGLMIEATTGNVEVAVYLTDPVNPQTVQSLTSKLDDLTVVSNVQYETKQQACDRF
                     KEIFKNQKALVENVDCSALPASLRVKLSDPEQYRQVAAVLQGQPGIERIVDQSRFLER
                     LFAVARVFRVGGLVISFVMLIAAAVLIANTVRMGLFARRKEIGIMRLVGATNWRIRLP
                     FLVEGLFESLVGATAAILFLFALKVAFINPLYNKILFIPWVTNNDVIATIPWLLVAGA
                     AVALVAGLAGMRRFLDV"
     gene            complement(2285..2962)
                     /gene="ftsE"
                     /locus_tag="VE646_00390"
     CDS             complement(2285..2962)
                     /gene="ftsE"
                     /locus_tag="VE646_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HZD78487.1"
                     /translation="MISLQRVTKIYKNGVAALDDVNVEVEKGEFVFIVGASGSGKSTF
                     IRLLLKEEEASKGEIFVAGKNLAKLTPWKIPHLRRNIGTVFQDFKLLQDKTVFENVAF
                     ALEVIGKPKPVIDQRVPEILELVGLGDKLNNFPDELSGGEQQRVSIARAVVNRPLVLL
                     ADEPTGNLDPTTSVDIMRLLDKVNRIGTTVVMATHDQAIVDAMRRRVIELECGKVIRD
                     QSRGVYS"
     gene            3098..5575
                     /locus_tag="VE646_00395"
     CDS             3098..5575
                     /locus_tag="VE646_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78488.1"
                     /translation="MRTLTRGDALPEEVAAGARPSWLRGFLVAGAGPMLIVVSVLIAL
                     RGFAFSDLLTNQHPDILSFWLPRSCFLGRSLSAGHLPLWNPFEMAGTPFAADPQSGWL
                     YLPAMLTSWLFGCGGGLRAFIVLQPILAGVGLWWFLRREGLIRPVATVGGLSLAMMIA
                     ATNAAISLPFAGTLAWTPFVLVGASGYLSAHRWVRRLPWLALAALAWGQVASAHMSHG
                     LVMATGLTFAYLVARSIAQIAAGERSVWGAAWVTVAFLAFLPLANLAILLPRLALIGR
                     SSLRGGYASLGGTLARAAGAEDRPLPTNGVWSAWPFSIAVTPGTYAGAAVLLLIPAAF
                     RSRRHRALVLAVGATGLVAYLFTLNLFVSAGWLRAAVLKLPFGDVYLHNPGRLRYLEL
                     LVAPILGAVGLQGFLDRLPSPRETWWWIGGGAALFLGLPLALGADPFRFILPAIGFAA
                     TVPVLWWLRRGRRWAVLLLPTVLASELLGNALWASAYEGGTVYTGLQSGDHPNLSAQP
                     LRWPDVHLDEYLRPGPIARYLQEQPTEQGRYLAWIPPAAYFNKGYLFTQGPHDWPALL
                     LGRALLFGLDDALGYSPIQLPRYWSYIRATNRNPVFYNASVIQLPDLADVRLLGLRYL
                     VLPKGVVPTYPRRDVPTVPGHVVASEFGYDLYEVQGWEPRASVVPDPTRVDGGVAALD
                     AVLGPSFDPARQAVVEQAPDLSPSGSSGTPGTASYRERSPEDVRITVHAGAPSLLVVR
                     NAWDRGWSATVDGRPAPVLRADYFLQGIPVPAGTHEVRLTYADRTIGRGLLASGLLWG
                     GLVVLGLALAADLRRRSPRREDTKPRA"
     gene            complement(5579..6698)
                     /gene="prfB"
                     /locus_tag="VE646_00400"
     CDS             complement(join(5579..6640,6642..6698))
                     /gene="prfB"
                     /locus_tag="VE646_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455867.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HZD78489.1"
                     /translation="MSEHTEHLEDLSKRLASAKSTFDVEGKRAQAAELERQAADPTLW
                     DDPAHGQELTTRLSRLRADVDRYDAFERRLADARAMDELLAEETDPDLQRELAQSVKS
                     LRAELDKLELATLLGGEYDRNDAIATLHAGAGGTESQDWADMLLRMYLRWAERERFSV
                     ELDEVLPGEEAGVKSATFIVHGSNAYGLLSTERGVHRLVRISPFDQQKRRHTSFASLD
                     VIPLLAETDEEDIEIDPKDLRIDVYRSTGPGGQSVNTTDSAVRITHLPTQITAACQNE
                     RSQLQNRAVAMRILKARLAERMRAEQQQKIEDLRGERRGIDFGSQIRSYVLAPYRLVK
                     DHRTDVEIGDVDRVLDGDLEPLIEAELRRRAESDGQRG"
     gene            complement(6747..9425)
                     /gene="secA"
                     /locus_tag="VE646_00405"
     CDS             complement(6747..9425)
                     /gene="secA"
                     /locus_tag="VE646_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HZD78490.1"
                     /translation="MPVVIEKLGSLGEGRRLKRLQQLVGIVNTYEPEIEDFSEQELRG
                     KSDEFRQRLADDQSLDDIMAEAFACVREAAKRTIGQRHFDVQVMGAMVLHEGKIAEMK
                     TGEGKTLVSTMPAFLNALAGSGVHIVTVNDYLAKRDSEWMGPIYRALGVSVGLIQASM
                     TPEERRPAYAADITYGTNNEFGFDYLRDNMAMRREEMVQRGHPYAIVDEVDSILIDEA
                     RTPLIISGMVADSAKWYQTFARLVPRLRRDEDYEVDEAKHTVAVTESGVAKVEEALGI
                     ENLYEHVHTPLVHHLQNALRAKELYKRDVDYIVTSGEVKIVDEFTGRVLEGRRYSEGL
                     HQAIEAKEGVRVKEENQTLATITIQNYFKMYEKLAGMTGTAKTQLAEFQEVYKLDVVE
                     IPTNRPLIRADEQDFIYKNEDAKWNSVVEDILERHEQGQPILVGTVSIEKSEKLSGYL
                     NRRGVSHNVLNAKNHEKEAMIVAQAGSRGSVTVATNMAGRGVDILLGGNPEYLARQEM
                     AAREFDNDRYLMFEMDPEERADYEAEYEPIYRKFKEHTDREHAEVVELGGLYVLGTER
                     HESRRIDNQLRGRSGRQGDPGESRFYLSLEDDLMRMFASDRVSSIMERLKWPEGEPIT
                     ARMVTRAVEGAQKQIEELNFERRKNILKYDDVMNTQRQVIYEERSRLLKGEDFKDQAL
                     DMVTDVVERTIQQWVGDKAFAEEWDLDALAAALGEVYPIRLTREQLEEAGTVPELTEL
                     VLSDALDVYEDKERTLGSGVMRELERMVLLNITDNKWREHLYEMDYLQEGIHLRAYGQ
                     RDPLTEWQREAYTMFEELTQSIRDEFVRYAYRVELVRQDEPSRPRPQRVVTSRGEDEQ
                     VNTGAAPQAVSQKVPRNAPCPCGSGKKYKKCHGATA"
     gene            complement(9518..>10053)
                     /locus_tag="VE646_00410"
     CDS             complement(9518..>10053)
                     /locus_tag="VE646_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD78491.1"
                     /translation="KAADLLPDIVLMDVRMPKRGGIEACTSIKEVAPSAKIIMLTISD
                     EEADLYEAIKAGASGYLLKEIPIEEVADAIRTVWSGQSRISPSMASKLLNEFASMTKV
                     SEERPQVPVPRLTDREMEVLRLVAKGMNNRDIAKELYISENTVKNHIRNILEKLHLHS
                     RMEAVVYAVREKMIEIT"
BASE COUNT         1359 a         3598 c         3317 g         1779 t
ORIGIN      
        1 ctggcatccg cgcggaagac gccctcacat gggcgctaat gatcatcgac cgatacgagc
       61 gcgccttcac acctccaaca gcgctcaggc acggggactg agcgttcttg gaggtggggg
      121 gagtcgaacc cccgtccctc ggcgaccgtt cggggcttct ccgggcgcag ccggcggtga
      181 gatctcgcct cggggctccc accggcggag cgacccctcg gccagcccgg gttcggtgtc
      241 cggcggcggc ccccgagcgg aaccgcaacc gtgagcctgc tcacgacgcc cgacccccga
      301 ccgcaggcgg atcggggcgg acggctacct aactagttag gcagcgagcg cgtattcagt
      361 atcggcgctt gtgttgatcc cggcttttat cgaggcatcc ggggacctcg gcccgcttca
      421 cccgagcggt acccgcccag gtcgaatccg gtgcaccccc atatgcagtt gcttcaagta
      481 tacgagagcg gtgggaggct cctcgcgtag cggtgcatcc gtgcgtcgtg ctcgtacccg
      541 acctcgcgcc aaaacccggc cgccgcctcc tccgaagaga tcaccagcgc gctgatccgt
      601 cgcgcgccga gctcgcgcag gcgccgttcc ccttccgcca ccagcgcggt ggcgatgccc
      661 cgccgacgca tcgagggaac caccgccagc cggtacatcg acccccgcca cccatcccag
      721 cccaccacga gcgtgcccac cagccgacca tcggcgtcgg ctaccagcag cgcgtgcgga
      781 tcgcgcaccg ggagccgagc caggccgacc gggtcgtcgg tcgggctctc atccgcgccg
      841 gcctcccacc acacgcggag caccgcatcc agctcttcgg gccgggcgtc gcggatcacc
      901 gggctcaccg tcggcgtcgg gcggcctgct ccatctcgcg cttgtgctcg cgctcggcga
      961 tggtctgccg cttctcgtat tgccgctttc ctcgggccag ggcgagctcc agcttggcca
     1021 ggccgtgtgc gaagtagacg cgcaggggga cgagggcgag cccgcgctcg gcggtcttgc
     1081 ccacgagccg ctcgatctgg tcccggcgca acaggagctt gcggatccga accgggtcat
     1141 acgtgcgctt gtcgccctgc tggtagggcg ggatgtgcat cccctccagc cacacctcgc
     1201 cgtcgcggat ccgcgcgtag gcctcggcca gggacccccg gcccccgcgc agcgacttca
     1261 cctcggagcc ggtcagcgcc atcccggcct cgcaccgctc caggacctcg tagtcgtgca
     1321 gggctcgccg gttggagacg acggtcttct gacccggggc gctgcttctg ctgggactca
     1381 cggccccgta agggtagccg cgctagacgt ccaggaaccg gcgcatgccg gccagcccgg
     1441 ccaccaacgc caccgccgcg ccggccacca agagccaggg gatcgtggcg atcacgtcgt
     1501 tgttcgtgac ccacgggatg aacaggatct tgttgtacag cgggttgatg aaggcgacct
     1561 tcagggcgaa gaggaacaag atcgcggcgg tcgcgcccac caggctctcg aacaggccct
     1621 ccaccaggaa gggcaatcgg atccgccagt tcgtggctcc aaccaaccgc atgatcccga
     1681 tctccttgcg ccgggcgaac agccccatcc gcaccgtgtt cgcgatcagc accgccgcgg
     1741 cgatcagcat cacgaaggag atcaccaggc cccccacccg gaacacccgg gccaccgcga
     1801 acagccgctc caggaaccgg ctctgatcca cgatccgctc gatcccgggc tgcccttgca
     1861 ggaccgccgc cacctgccgg tactgctccg gatccgagag cttcactcgc agggaggccg
     1921 ggagcgccga gcagtcgacg ttctccacca gcgccttctg gttcttgaag atctccttga
     1981 agcgatcgca cgcctgctgc ttggtctcgt attgcacgtt ggagacgacg gtgaggtcgt
     2041 ccagcttcga ggtgagcgac tggaccgtct gtgggttgac cggatcggtc aggtagactg
     2101 caacctccac gttcccggtg gtggcctcga tcatcaggcc gacctgacgc gtgatgagca
     2161 tggccagacc gaacagcagc agcgcgatga acgtggtgga cacggcggcg aacgccacca
     2221 ggccgttccg ccgcaggccc aggctgacct cccggaacag gtagtcgaac cggcgcccgg
     2281 tcacctagct gtacacccct cgggactggt cccggatcac ctttccgcat tccagctcga
     2341 tcacccggcg gcgcatcgcg tccacgatcg cctggtcgtg ggtggccatc accacggtgg
     2401 tgccgatgcg gttcactttg tccagcagcc gcatgatgtc cacggacgtg gtggggtcca
     2461 ggttgccggt gggctcgtcg gccagcagga ccagcggccg gttcaccacg gcgcgcgcga
     2521 tcgacacccg ctgctgctcc ccgcctgaga gctcgtccgg gaagttgttc agcttgtccc
     2581 ccagcccgac cagctcgagg atctccggca cgcgctggtc gatgaccggc ttgggcttgc
     2641 cgatcacctc cagcgcgaag gccacgttct cgaacacggt cttgtcctgg agcagcttga
     2701 agtcctggaa gacggtcccg atgttccgtc gcaggtgcgg gatcttccac ggggtcagct
     2761 tggccaggtt cttgccggcc acgaagatct cgcccttgct ggcctcctcc tccttcagca
     2821 ggaggcggat gaacgtcgac ttgcccgagc cggacgcacc cacgatgaag acgaactcgc
     2881 ccttctcgac ctcgacgttg acgtcgtcga gcgcggcgac cccgttcttg tagatcttgg
     2941 tgacgcgctg aagactgatc atctcgagtg ctcaggggag aagttggggg gttctgaccg
     3001 ccgcgcagtc tagcaggtac ctccccggaa cggaacgaag ctcgaccgtt ggttcggcac
     3061 cctcgaagcg cacccacctc cactacgctg gggaacgatg cgcaccctca ccaggggcga
     3121 cgccctgccc gaggaggtcg cagccggggc acgcccctcg tggctccggg gcttcctggt
     3181 ggccggggcc ggcccgatgc tgatcgtggt gtcggtgctg atcgcgctcc gcggcttcgc
     3241 cttctcggac ctgttgacca accagcaccc cgacatcctc tccttctggc tcccccgctc
     3301 gtgcttcctg ggtcggagcc tgtcggccgg gcaccttccg ctgtggaacc cgttcgagat
     3361 ggcggggacg cccttcgcgg ccgatccgca atcgggctgg ctctacctgc cggccatgct
     3421 cacctcctgg ctgttcgggt gtggtggcgg gctgcgggcc ttcatcgtgc tgcagccgat
     3481 cctggccggg gtgggcctgt ggtggttcct gcgccgcgag ggcctgatcc gtccggtggc
     3541 gaccgtgggc gggctgtcgt tggccatgat gatcgccgcc accaacgcgg ccatctcgtt
     3601 gccgttcgcc gggacgctgg cctggacgcc cttcgtactg gtgggcgcct cggggtacct
     3661 gagcgcccat cggtgggtcc gtcggctgcc ctggctggcc ctggccgcgc tggcctgggg
     3721 gcaggtggcg agcgcccaca tgtcccatgg actggtcatg gcgaccgggc tcacgttcgc
     3781 gtacctggtg gcccgttcga tcgcccagat cgcggccggc gagcgctcgg tgtggggcgc
     3841 cgcctgggtc acggtggcgt tcctggcctt cctgcccctc gcgaacctgg ccatcctgct
     3901 cccccggctg gccctgatcg gccggtccag cctgcgcggc gggtacgcct cgctgggcgg
     3961 aaccctggcc cgagccgcgg gcgcggagga tcggccgctg cccacgaacg gcgtctggag
     4021 cgcctggccg ttctccatcg cggtaacgcc cgggacgtac gcgggggccg ccgtgctgtt
     4081 gctgatcccc gcggcgttcc gctcccgccg gcaccgcgcc ctggtcctgg ccgtgggcgc
     4141 cacgggcctg gtggcctacc tgttcacgct gaacctgttc gtgagtgcgg gctggctgcg
     4201 ggcggccgtc ctgaagctgc cgttcgggga cgtctacctg cacaacccgg gacggctgcg
     4261 atacctggaa ctgctggtgg cgccgatcct gggggcggtg gggctccagg ggttcctcga
     4321 ccgcctgccc tcccctcggg agacctggtg gtggatcggg gggggcgccg ccctgttcct
     4381 ggggctgccc ctggccctgg gggccgaccc cttccggttc atcctgccgg ccatcggctt
     4441 cgcggcgacg gtgccggtcc tctggtggct ccggcgcggg cggcggtggg cggtgctgct
     4501 gctgccgacg gtgctcgcct ccgagctgtt ggggaacgcc ctgtgggcct cggcgtacga
     4561 gggcggcacc gtgtacacgg ggctgcagag cggcgaccat cccaacctgt ccgcccagcc
     4621 cctgcggtgg cccgacgtcc acctggacga gtacctgcgg cccggcccca tcgcccgtta
     4681 cctgcaggag cagccgaccg agcagggccg ctacctggcc tggatcccgc cggccgcgta
     4741 cttcaacaag ggctacctct tcacgcaggg gccgcacgac tggccggcgc tgctgctggg
     4801 ccgggccctg ctgttcggcc tggacgatgc gctcggatac tcgccgatcc agctcccccg
     4861 gtactggtcg tacatccggg ccacgaaccg aaaccccgtc ttctacaacg cctccgtgat
     4921 ccagctcccc gacctggccg atgtgcggct gctcgggctc cgatacctcg tcctgcccaa
     4981 gggggtggtc cccacgtacc ccaggcgcga cgtccccacg gtgccggggc acgtcgtggc
     5041 ctccgagttc ggctacgacc tgtacgaggt ccaggggtgg gagccccggg cctccgtggt
     5101 cccggacccc acacgggtcg atgggggcgt ggcggccctg gacgcggtgc tcggcccgag
     5161 cttcgacccc gcgcgacagg ccgtggtgga gcaggctccg gacctgtccc cgtccggctc
     5221 ctcggggacg cccggcaccg cctcctaccg cgagcgctcg cccgaggacg ttcggatcac
     5281 cgtgcacgcc ggggcgccct cgctcctggt ggtccgcaac gcttgggacc ggggctggtc
     5341 ggccacggtg gacggccggc ccgccccggt gctccgcgcc gactacttcc tgcaggggat
     5401 ccccgtcccc gccggcaccc acgaggtgcg cctcacgtat gccgaccgca cgatcggacg
     5461 tggcctcctg gcctcgggcc tgctgtgggg gggcttggtc gtcctcggcc tggccctggc
     5521 cgccgacctt cggcgacgct caccgaggag ggaagacacg aagccccgag cctgagccct
     5581 acccgcgctg gccgtccgac tcggcccggc gccgcagctc ggcctcgatc agcggctcga
     5641 ggtcgccgtc cagcacccgg tccacgtcgc cgatctccac atcggtgcga tggtccttca
     5701 ccaagcggta cggggccagc acgtaagagc ggatctgcga gccgaagtcg atcccccgtc
     5761 gctccccgcg taggtcctcg atcttctgct gctgctcggc acgcatgcgc tcggccagcc
     5821 gggccttcag gatccgcatg gccacggctc ggttctgcag ctgggagcgt tcgttctggc
     5881 aggctgcggt gatctgggtg ggaaggtgcg tgatccgtac ggccgagtcg gtggtgttca
     5941 cggactgacc tccggggccc gtggagcggt acacgtcgat ccgcaggtcc ttgggatcga
     6001 tctcgatgtc ctcctcgtcg gtctccgcca gcagcgggat gacgtccagg ctggcgaacg
     6061 aggtgtgacg gcgcttctgc tggtcgaacg gcgagatccg caccaggcgg tgaaccccac
     6121 gctcggtgga gagcaggccg taggcgttgg acccgtgcac gatgaaggtg gcgctcttca
     6181 cccccgcctc ttcgccgggc agcacctcgt ccagctccac cgagaaccgc tcgcgctccg
     6241 cccagcgcag gtacatccgc agcagcatgt ccgcccaatc ctgagattcg gtgccgcccg
     6301 cgccggcatg gagcgtggca atcgcgtcgt tgcggtcgta ctcgccgccc agaagggtgg
     6361 ccagctccag cttgtcgagc tccgctcgca ggctcttcac cgactgcgcc agctcccgtt
     6421 ggagatcggg gtcggtctcc tcggccagga gctcgtccat ggcccgggcg tcggccaggc
     6481 ggcgttcgaa ggcgtcgtag cggtccacgt cggcgcggag ccgcgacagg cgagtggtca
     6541 gctcctgccc gtgggccgga tcgtcccaga gcgtgggatc ggccgcctgc cgctcgagct
     6601 cggccgcctg cgcccgcttg ccctcgacgt caaaggtact ccttcgccga ggcaagccgc
     6661 ttgctgagat cctccaggtg ctccgtgtgc tcgctcacga gggcatggta ccggccggtg
     6721 aggcacggcg gcgctgaggg cggcgatcag gccgtggccc cgtggcactt cttgtacttc
     6781 ttgcccgagc cgcaggggca gggggcgttg cgcggcacct tctggctcac cgcctgcggc
     6841 gcggcgcccg tgttgacctg ctcatcctcg ccgcggctgg tcaccacgcg ctggggccga
     6901 ggccgtgacg gctcgtcctg ccgcaccagt tccacacggt acgcgtaccg cacgaactcg
     6961 tcgcggatgc tctgggtgag ctcctcgaac atcgtgtacg cctcgcgctg ccactcggtg
     7021 aggggatcgc gctgaccgta cgcgcgcagg tggatgccct cctgcaggta gtccatctcg
     7081 tagaggtgct cgcgccactt gttgtcggtg atgttgagca ggaccatccg ctcgagctcg
     7141 cgcatcaccc ccgagcccag ggtccgttcc ttgtcctcgt acacgtccag ggcatcggac
     7201 aggacgagct cggtcagttc cggcaccgtg ccggcctcct ccagctgctc gcgggtgagc
     7261 cggatggggt agacctcgcc cagggcggcg gccagggcgt ccaggtccca ctcctcggcg
     7321 aaggccttgt cccccaccca ttgctggatg gtccgctcca ccacgtcggt gaccatgtcg
     7381 agggcctgat ccttgaagtc ctcccccttc aggagccggg aacgctcctc gtagatcacc
     7441 tggcgctggg tgttcatcac gtcgtcgtac ttcaggatgt tcttccgacg ctcgaagttg
     7501 agctcctcga tctgtttctg cgcgccctcc accgcccggg tgaccatcct ggccgtgatg
     7561 ggctcgccct ccggccactt caggcgctcc atgatggagg acacccggtc gctggcgaac
     7621 atccgcatca ggtcgtcctc gagcgacagg tagaaccggg actcgccggg gtcgccctga
     7681 cggcccgacc ggcctcgaag ctggttgtcg atgcgccggg attcgtgccg ctcggtgccc
     7741 agcacgtaca ggccccccag ctcgacgacc tccgcgtgct ctcggtccgt gtgctccttg
     7801 aacttgcggt agatcggctc gtactcggcc tcgtaatcgg cgcgctcctc cggatccatc
     7861 tcgaacatca ggtagcggtc gttgtcgaac tccctggccg ccatctcctg gcgcgcgagg
     7921 tactcggggt tgccacccag caggatgtcg acgcctcggc cggccatgtt cgtggccacg
     7981 gtgaccgagc cacggctccc ggcctgggcc acgatcatgg cctccttctc gtggttcttc
     8041 gcgttcagga cgttgtggga cacgccacgg cggttcaggt agcccgacag cttctcggac
     8101 ttctcgatcg agaccgtgcc gaccaggatc ggctgcccct gctcgtggcg ctcgaggatg
     8161 tcctcgacca ccgagttcca cttggcgtcc tcgttcttgt agatgaagtc ctgctcgtcg
     8221 gcgcggatca gcggccggtt ggtggggatc tcgaccacgt cgagcttgta gacctcctgg
     8281 aactcggcca gctgggtctt ggccgtcccg gtcattccgg cgagcttctc gtacatcttg
     8341 aagtagttct ggatggtgat ggtggccagg gtctggttct cctccttgac ccggaccccc
     8401 tccttggcct cgatcgcctg gtggagcccc tccgagtacc gccggccctc cagcacgcgg
     8461 ccggtgaact cgtcgacgat cttcacctcc ccgctggtca cgatgtagtc gacgtcgcgc
     8521 ttgtagagct ccttggcccg caaggcgttc tgcaggtgat gcacgagcgg cgtgtggacg
     8581 tgctcgtaga ggttctcgat cccgagcgcc tcctccacct tggccacacc ggattcggtc
     8641 acagccaccg tgtgcttggc ctcatcgacc tcgtagtcct cgtctcgccg caggcgaggg
     8701 accagccggg cgaaggtctg gtaccacttg gccgagtcgg ccaccatccc gctgatgatc
     8761 agcggggtcc gcgcctcgtc gatcaggatg gagtcgacct cgtcgacgat ggcgtaggga
     8821 tgcccgcgct gcaccatctc ctcccggcgc atcgccatgt tgtcgcgcag gtagtcgaag
     8881 ccgaactcgt tgttcgtccc gtaggtgatg tccgccgcgt aggcggggcg gcgctcctcg
     8941 ggcgtcatcg acgcctggat cagacccacc gagacgccca gggcccggta gatcggaccc
     9001 atccactccg agtcccgctt ggccaagtag tcgttgaccg tcacgatgtg cacgccgctg
     9061 ccggccaggg cgttcaggaa ggcgggcatg gtggagacga gggtcttgcc ctcaccggtc
     9121 ttcatctcgg cgatcttgcc ctcgtgcagg accatcgccc ccatgacctg cacgtcgaag
     9181 tggcgctgtc cgatggtgcg cttggcggct tcccgcacgc aggcgaacgc ctcggccatg
     9241 atgtcgtcca gcgactgatc gtccgccagc cgctggcgga actcgtcgga cttgccccgg
     9301 agctcctgct cggagaagtc ctcgatctcc ggctcgtagg tgttgacgat ccccacgagc
     9361 tgctggagcc gcttcagcct gcgaccctct ccgagcgaac cgagcttctc gatgaccacg
     9421 gggatccttc cgggggggga ggggtttcac ccgattctat cgaaggaccg cgcacgccgg
     9481 acccacgagg gcccgatccg gccgggacgc cttcggctca ggtgatctcg atcatcttct
     9541 cccgcacggc gtacacgacg gcctccatgc gggagtgcag gtgcagcttc tccaggatgt
     9601 tcctgatgtg gttcttcacc gtgttctcgg agatgtagag ctccttggcg atgtcgcggt
     9661 tgttcatgcc cttggccacg agcctgagca cctccatctc ccggtccgtg agcctgggga
     9721 ccgggacctg ggggcgctcc tcggacacct tggtcatgga ggcgaactcg ttcaggagct
     9781 tggaggccat ggagggcgag atccgcgact ggcccgacca caccgtccgg atcgcatcgg
     9841 cgacctcctc gatggggatc tccttcagca ggtacccgga cgctcccgcc ttgatcgcct
     9901 cgtagaggtc ggcctcctcg tcgctgatcg tcagcatgat gatcttggcg ctgggggcga
     9961 cctccttgat ggaggtgcac gcctcgatcc cgccccgctt cggcatccgc acgtccatca
    10021 ggacgatgtc gggcagcagg tcggcggcct tgt
//
LOCUS       DASQQQ010000010         7198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_28045, whole genome shotgun sequence.
ACCESSION   DASQQQ010000010 DASQQQ010000000
VERSION     DASQQQ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28045"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VE646_00415"
     CDS             complement(<1..366)
                     /locus_tag="VE646_00415"
                     /inference="COORDINATES: protein motif:HMM:NF025384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HZD78492.1"
                     /translation="MAETVTIAGRSYLKRNPLGVLGLTFITAGIYGLYWFYKVNEEIQ
                     RFTGDDTISPTRSLMAVLFGWLVIVPPFIAWYNTSNHVRQMEEQVGIPQQIEPALAIV
                     ILLVISLLGGVYVQEHLNRA"
     gene            complement(393..1307)
                     /locus_tag="VE646_00420"
     CDS             complement(393..1307)
                     /locus_tag="VE646_00420"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HZD78493.1"
                     /translation="MTSSGSRLERILRSGRFCVTAELVPPRSAGPGPLRAQARSLVGY
                     ADAVNVTDNPTPSARMSPLAGVAVLAASGLEPILQLTCRDRNRLALTADLLGGWALGA
                     RGVLCLSGDPVQRGDHPDAAEVRDLSVLELVGLAVALRQGRTLSGGEVEEPPRYLIGV
                     ADSPLAPGYDPSRLEAKIDAGADFVQTQIVYDVEAFAAWADLVRPRGVLERASVIAGV
                     VPLRSVRQARFLDGLPGVRVPPGLIRRLEQAGPDAAEVGSALTVDLLAELQRVPHVAG
                     VHLMALGKDGLGQVIEAAGLLPRPTVAA"
     gene            complement(1304..2170)
                     /locus_tag="VE646_00425"
     CDS             complement(1304..2170)
                     /locus_tag="VE646_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HZD78494.1"
                     /translation="MTATIMDGRAIGAQIRSEVAERVKALAERGITPGLAAVLVGDDE
                     ASRIYLGAKHKACADVGMRSEQLDLPAYVTEGELLAALRRLNRDPDIHGIIVQLPVPK
                     HISELAIQTTIDPNKDVDGLHPLNVGLMVRGEPSFQPATPYGIVELLLRSGVELDGAE
                     VVVVGYGGLVGAPLSIMLAQDSIRGNATVTICHVRTRDLAVHTRQADILIAAAGVPEL
                     ITADMVKPGAVVIDVGVHRTDTGLVGDVRFDEVREVAGMISPVPGGVGPMTTAMLLLN
                     AVTATEKLTERH"
     gene            complement(2228..3766)
                     /gene="purH"
                     /locus_tag="VE646_00430"
     CDS             complement(2228..3766)
                     /gene="purH"
                     /locus_tag="VE646_00430"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003961555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZD78495.1"
                     /translation="MSEVRPVRRALLAVYDKGGLVELARALVDLGVALVSSGGTAAAL
                     RAAGVPVTEVQEVTGFPEMLEGRVKTLHPRIHAGILADRRKPEHERQLADRGIEPFDL
                     VVVNLYPFRETVAAGAGPDEVIEKIDIGGPAMVRAAAKNFESVAVVASPERYERVLSE
                     LRERGGLGRETRRELAAEAFAQTAAYDAAVAAWFGARPGALPGFVGLPLEKVEDLRYG
                     ENPHQRGALYRQIGGPGVLAGARVLRAAKPMSFNNWLDLDAAYALAGAFGETAAVVVK
                     HNNPCGAALAATPADAYRRAFESDTVSAFGGIVAFNREVDGAAAEAMADVFTEVVVGP
                     SFTAAALEAFAARSSLRVVQAPLPAGESLEVRPIPGGALVQDRDAVRETRAAMEVVSA
                     REPTEEEWRDLAFAWTVAARVKSNAIVFAKDLATVGIGAGQMSRVDASWLAVRKSEGR
                     AKEAVMASDAFFPFPDAVEVAADAGVTAVVHPGGSVRDEEVLRAAEARDMAVVLTGHR
                     HFRH"
     gene            complement(3763..4368)
                     /gene="purN"
                     /locus_tag="VE646_00435"
     CDS             complement(3763..4368)
                     /gene="purN"
                     /locus_tag="VE646_00435"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZD78496.1"
                     /translation="MNARVAVLASGEGTNLQALLDDPVAGPWVVLVISDHEGARALER
                     ARRAGVEAVWLDPAAHRDRSSFDLALRDLLGSKGIEVLALAGYMRILGPEVVRAFDGR
                     TLNVHPALLPAFPGTDSMRRALERGVKVTGVTVHLVDEEVDHGPIVSQEAVTVLPGDD
                     WDALEQRIHEVEHRLLPAAVRALVEGRLKVEGGIVHVLEAP"
     gene            4430..5065
                     /locus_tag="VE646_00440"
     CDS             4430..5065
                     /locus_tag="VE646_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZD78497.1"
                     /translation="MGAPGVVVFDLGGVVIDWDPRHLYRKLFPDPSQMERFLSEVCTP
                     EWNEGFDRGLPFQEGVERLAAERPEWAEPIRAYRDRWPEMLAGTIPGAPELLRELREE
                     GVRLFALSNWSTETFRFAVDRFGILDLFEGILLSGQVGATKPSIRLFQAFLERFGLDP
                     QGCVFVDDRPDNVQAARALGFRALTFRSAGGLRSDLVDLGLLGPGAEPDRR"
     gene            5075..5671
                     /locus_tag="VE646_00445"
     CDS             5075..5671
                     /locus_tag="VE646_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78498.1"
                     /translation="MPQERLVRGADRCQMHAGSPAVARCDGCGRRLCLACAVPVRGHI
                     FGPECLARALGTEAGTDKAPQAVPEARVGSLVRGVALAVAWAATLLPWSRFGEGAGWF
                     GAWGRSRWSLLAAVASAAGLLLWLIARRMPSLRGRGWELALGLLGLLTAVGGVMAAVH
                     PPFASRAWFAPWIAAGAGLAVVATSLAPERGPRRDAGI"
     gene            5757..6242
                     /locus_tag="VE646_00450"
     CDS             5757..6242
                     /locus_tag="VE646_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78499.1"
                     /translation="MAQSAGFDMSRVSTATKILGGAAVLLLIDSFLAWQRVCVSFLTV
                     SACGSANMWGGRGSFFGIIAGILTIVLIAWEAFNLAGSNSLNLSASPGKISAYLGFGV
                     LAFVVVKFILALTNHPAFGAWIGMILALAVGYGAWMKFQEPETRPGPMMGSGDNTGGM
                     A"
     gene            6368..6955
                     /locus_tag="VE646_00455"
     CDS             6368..6955
                     /locus_tag="VE646_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78500.1"
                     /translation="MKRCPYCAEEIQDQAIKCRWCGSMLVDLPAPPGGGPTEPSEQAL
                     QYTHSGRRYLLGYGPDHFGIWDRERPGGPVERFPRTDEGWRSAWTRFDALEPDHTEVA
                     ITSGAGPGATTGSPSSGWEPRGGAAAGARAPRPVSGAWWLLPIFMGWLGGLIAWLVNR
                     DADPARARAMLITGILISVVILFLFIAAGSGSSGA"
BASE COUNT          964 a         2687 c         2542 g         1005 t
ORIGIN      
        1 cgcccggttc aggtgctcct ggacgtagac gccgccgagc aggctgatca ccagcaggat
       61 cacgatggcc agcgccggct cgatctgctg ggggatgccc acctgctcct ccatctgccg
      121 gacgtggttg gaggtgttgt accaagcgat gaagggcggc acgatcacca gccacccgaa
      181 cagcacggcc atcagcgagc gggtcgggct gatcgtgtcg tcgcccgtga accgctggat
      241 ctcctcgttc accttgtaga accagtacag gccgtagatg cccgccgtga tgaaggtgag
      301 ccccagcacg ccgagcgggt tgcgcttcag gtacgaccga cccgcgatgg tgaccgtctc
      361 ggccatctcg ccccctttcc ctcgaccgaa gtctaggcgg cgacggtggg ccggggcagg
      421 agcccggcgg cctcgatcac ctgccccagc ccatccttgc ccagcgccat cagatgcacc
      481 ccggccacgt ggggcacccg ctgcagctcc gcgagcaggt ccacggtgag cgccgacccc
      541 acctccgcgg cgtcgggtcc ggcctgctcc aggcggcgga tcaggccggg agggacgcgc
      601 acgccgggca ggccgtcgag gaaccgcgcc tgccgaacgc tccgcagggg caccacaccg
      661 gcgatcacgg aggcccgctc cagcaccccg cgcggacgga ccaggtcggc ccatgcggcg
      721 aaggcctcca cgtcgtacac gatctgggtc tgcacgaagt ccgccccggc atcgatcttg
      781 gcctccaggc ggctcgggtc gtagccgggg gcgagggggg agtcggcgac cccgatcagg
      841 taccggggag gctcctccac ctcccccccg gacagcgtcc gcccttgccg cagagcgacc
      901 gccaggccga ccagctccag gacggagagg tcacggacct ccgcggcgtc cgggtgatcg
      961 ccccgctgca cggggtcgcc ggacaggcac aggacccccc gggccccgag ggcccagccc
     1021 ccgagcagat cggcggtcag ggcgagccgg ttccggtccc ggcacgtgag ctgcaggatc
     1081 ggctcgagcc ccgatgccgc caggaccgcc accccggcca ggggggacat gcgggcggag
     1141 ggcgtggggt tgtcggtcac gttcaccgcg tccgcgtagc ccaccagcga ccgggcctgc
     1201 gcccggagcg gacccgggcc cgccgaccgc ggcggcacca gctcggcggt gacgcagaag
     1261 cggcccgagc gcaggatccg ttcgagccgg ctgccggacg aggtcaatgg cgctcggtga
     1321 gcttctcggt ggccgtgacc gcgttcagca gcagcatggc cgtggtcatg ggacccacgc
     1381 cgcccgggac cggggagatc atccccgcca cctcccgcac ctcgtcgaac cgcacgtccc
     1441 ccaccagccc ggtgtcggtg cgatggacgc cgacgtcgat caccaccgct cccggcttga
     1501 ccatgtcggc ggtgatcagc tcggggacgc cggcggcggc gatcaggatg tcggcctggc
     1561 gcgtgtgcac cgccaggtcc cgggtgcgca cgtggcagat cgtcacggtc gcgttgcccc
     1621 ggatcgagtc ctgggccagc atgatggaca gcggcgcccc caccagcccc ccatagccca
     1681 ccacgaccac ctccgcaccg tcgagctcca ccccggagcg gagcagcagc tcgacgatgc
     1741 cgtacggggt ggccggctgg aacgacggct ccccccggac catcaacccc acgttcaggg
     1801 gatgcagccc gtcgacgtcc ttgttgggat cgatcgtggt ctggatcgcc agctcggaga
     1861 tgtgcttggg caccggcagc tggacgatga tgccgtggat gtcggggtca cggttcagcc
     1921 ggcgcagggc ggccaggagc tcaccttcgg tcacgtaggc cggcaggtcc agctgctccg
     1981 agcgcatccc gacatcggca caggccttgt gtttggcccc caggtagatc ctcgacgcct
     2041 cgtcgtcgcc caccagcacc gcggccagcc ccggcgtgat gccgcgctcg gccagggcct
     2101 tgacgcgctc cgcgacctcg gagcggatct gcgccccgat ggccctgccg tccatgatgg
     2161 tggcggtcac ggctcggaat cctaccggtg ggtcggttcc cgggccatga ccgaaggtca
     2221 cgccggctca gtgacggaag tgccgatgcc ccgtcagcac gacggccatg tccctggcct
     2281 cggccgccct caggacctcc tcgtcccgga ccgacccacc cggatgcacc accgcggtga
     2341 cgcccgcgtc cgccgccacc tccaccgcgt cggggaaggg gaagaacgcg tcgctggcca
     2401 tgaccgcctc cttggcccgg ccctccgact tgcgcacggc gagccaggag gcatccacgc
     2461 gggacatctg cccggccccg atcccgacgg tcgccaggtc cttggcgaac acgatcgcgt
     2521 tggacttcac gcgcgcggcc accgtccagg cgaacgcgag gtcccgccac tcctcctccg
     2581 tgggctcccg ggccgagacg acctccatgg ccgctcgggt ctcgcggacg gcatcgcggt
     2641 cctggaccag cgcgccgccg gggattggac gaacctccag gctctcgccg gcggggagcg
     2701 gcgcctgcac cacccggagg ctcgagcgcg ccgcgaaggc ctcgagcgcc gccgccgtga
     2761 aggaggggcc caccaccacc tcggtgaaca cgtcggccat cgcctcggcc gccgccccgt
     2821 cgacctcgcg gttgaaggcc acgatcccac cgaaggccga gaccgtgtcc gactcgaagg
     2881 cccggcggta ggcatcggcg ggcgtggccg ccagggccgc gccgcacggg ttgttgtgct
     2941 tgaccacgac cgccgccgtc tccccgaagg cgcccgccaa ggcgtaggcg gcgtccaggt
     3001 ccagccagtt gttgaaggac atcggcttgg cggcccgcag gacccgcgcg cccgccagga
     3061 cgccgggacc gccgatctgt cggtacagcg cccctcgctg gtgtgggttc tccccgtacc
     3121 gcaggtcctc caccttctcc agcgggagcc ccacgaaccc cggcagggcg cccggccgtg
     3181 ccccgaacca ggcggccacg gccgcgtcgt acgccgcggt ctgcgcgaag gcctcggcgg
     3241 ccagctcgcg gcgggtctcc cgcccgagtc ctccccgctc ccggagctcg gacagcaccc
     3301 gttcgtaccg ctcgggcgag gccaccacgg ccaccgactc gaagttcttg gccgcggccc
     3361 ggaccatggc cggccctccg atgtcgatct tctcgatcac ctcgtccggc ccggcccccg
     3421 cggcgaccgt ctcgcggaag gggtacaggt tcaccaccac cagatcgaag ggctcgatcc
     3481 cccggtccgc cagctgccgc tcgtgctcgg gcttgcggcg atcggccagg atccccgcgt
     3541 ggattcgcgg gtggagggtc ttcacccgcc cctcgagcat ctcggggaag ccggtgacct
     3601 cctgaacctc ggtgaccggc acgcccgccg cccgcagggc cgccgccgtc ccccccgagg
     3661 acacgagcgc cacccccagg tcgaccagcg cccgtgcgag ctccacgagc ccgcccttgt
     3721 cgtagacggc caggagcgcc cggcgcacgg gtcggacctc gctcacggtg cctccagcac
     3781 gtggacgatc ccgccctcga ccttgagccg accttccacc agcgctcgga ccgccgccgg
     3841 caggagccga tgctcgacct cgtggatccg ctgctcgagc gcgtcccagt cgtcccccgg
     3901 gagcaccgtg accgcctcct gggagacgat cgggccgtgg tcgacctcct cgtccaccag
     3961 gtgcacggtg accccggtga ccttcacccc ccgctccagg gcccgcctca tcgagtcggt
     4021 gccggggaag gccggcaaga gcgccgggtg gacgttcagg gtcctgccgt cgaaggcccg
     4081 gaccacctcg ggaccgagga tccgcatgta gcccgcgagc gccagcacct cgatcccctt
     4141 cgaccccagg aggtcccgga gggcgaggtc gaacgaggat cggtcgcggt gagcggccgg
     4201 atccagccag acggcctcca cgccggcgcg ccgagcccgc tccagcgccc gagccccctc
     4261 gtgatcggag atcaccagga ccacccacgg cccagcgacc gggtcgtcca gcagggcctg
     4321 gaggttcgtc ccctcccccg aggcgagcac ggcgaccctg gcgttcactg agcgcaggct
     4381 agggacccgc cgccacccgc gtcaacgcgc ccgtcgctac cgtgatggca tgggggcgcc
     4441 gggggtggtg gtcttcgatc tggggggcgt ggtgatcgac tgggatcccc gccacctcta
     4501 ccgcaagctc ttccccgacc catcgcagat ggagcgcttc ctcagcgagg tctgtacccc
     4561 cgagtggaac gagggcttcg accggggcct gcccttccag gaaggggtgg agcgactggc
     4621 cgccgagcgt cccgagtggg ccgagccgat ccgcgcctac cgggaccggt ggcccgagat
     4681 gctggcgggg acgatccccg gagctcccga gctcctcagg gagctgcgcg aggagggggt
     4741 ccgcctcttc gccctgtcca actggtccac cgagaccttc cggttcgccg tggatcggtt
     4801 cgggatcctg gacctcttcg agggcatcct cctctcgggc caggtggggg ccaccaagcc
     4861 ctcgatccgc ctgttccagg cgttcctgga gcgcttcggc ctggacccgc agggatgcgt
     4921 cttcgtggac gaccgtccgg acaacgtgca ggcggcccgt gccctcggct tccgtgccct
     4981 caccttccga tcggcgggag ggctgcggag cgacctggtg gacctgggcc tgctgggtcc
     5041 cggtgcggag cccgaccggc gctaagctcc cgctgtgcca caggaacggc tggtcagggg
     5101 cgccgatcgg tgccagatgc acgcgggcag ccccgccgtg gctcgctgcg acgggtgcgg
     5161 ccgtcggctg tgcctcgcgt gcgccgtccc ggtcagggga cacatcttcg gacccgagtg
     5221 cctggcccgg gcgttgggga cggaggcggg caccgacaag gcgccgcagg ccgtgccgga
     5281 ggctcgggtc ggctccctgg tccggggggt ggctctggcc gtggcctggg cggccaccct
     5341 gctgccctgg agccggttcg gggagggcgc gggctggttc ggagcctggg gccgttcccg
     5401 ctggtcgctg ctcgccgccg tcgcgtcggc ggccggactg ctcctgtggc tgatcgctcg
     5461 ccggatgccc tctctccggg gacgtggctg ggaactggcg ctcgggctgc tcggcctgct
     5521 gaccgcggtg gggggggtga tggcggccgt gcacccgccg ttcgcctccc gagcctggtt
     5581 cgcgccgtgg atcgccgcgg gggcgggtct ggcggtcgtg gcgacctccc tcgcccccga
     5641 gcggggcccg cggagagatg ccggtatttg actccccccc gaccaggaca ttcaatacgc
     5701 ggggccgcgg tcctggtcct gtaccgtggt ccggaacccc gaaaggagtg gagggtatgg
     5761 cgcagagtgc gggcttcgac atgtcgaggg tgtcgacggc caccaagatc ctcggcgggg
     5821 cggcggtcct cttgctgatc gactcgttcc tagcctggca gcgggtctgt gtctcgttcc
     5881 tgacggtcag cgcctgcggc agcgccaaca tgtggggcgg cagaggaagc ttcttcggca
     5941 tcatcgccgg catcctcacg atcgtcctga tcgcctggga ggcgttcaac ctggcgggca
     6001 gcaactcgct caacctgagc gcatccccgg gcaagatcag cgcctatctg ggattcggcg
     6061 tcctggcgtt cgtggtcgtg aagttcatcc tcgccctcac caaccacccg gcgttcggcg
     6121 cgtggatcgg gatgatcctg gccctggcgg tggggtacgg agcctggatg aagttccagg
     6181 aacccgagac ccgtcccggc ccgatgatgg ggtccgggga caacaccggc ggcatggcgt
     6241 aacgttctcg ctcgaaccct cgaaggcccg gtcgtgaggc cgggccttcg ccgtccccgg
     6301 gccgggcggg gcgaggcctg ggcgcaggac ggctacgatg cgggtgaacc gtggacgggg
     6361 agcccggatg aagagatgcc cgtactgcgc ggaggagatc caggaccagg ccatcaagtg
     6421 ccggtggtgc ggcagcatgc tggtggacct gccggctccc ccgggcggcg gtcccacgga
     6481 gccctcggag caggccctcc agtacacgca ttcgggcagg cgctacctgc tcggctacgg
     6541 ccccgatcac ttcgggatct gggatcgaga gcgcccgggc ggaccggtcg agcgcttccc
     6601 ccggaccgat gagggttggc gttcggcctg gacacgtttc gatgccctgg agcccgacca
     6661 caccgaggtg gccatcacct ccggggccgg acccggggcc accaccggat cgccgtcctc
     6721 cgggtgggag ccgcgcgggg gcgcggcggc cggagcccgg gcccctcgcc cggtcagcgg
     6781 ggcttggtgg ctcctgccga tcttcatggg atggctgggc ggcctgatcg cctggctcgt
     6841 gaaccgtgac gccgatcccg ctcgggcccg ggccatgctg atcaccggca tcctgatcag
     6901 cgtggtgatc ctgttcctgt tcatcgctgc gggctcgggt tcatctggcg cgtagcctgc
     6961 cgcgcgcacc aggacgcggc tgcggtgccc gcgtgcggcg ggcgcggcga cgaccgcagc
     7021 tacaggcgct cgggttgccc cggcggcggg ggaggcagga gcggctcggt cggggtcggg
     7081 tccgcccccc accctgcagg ccccgccggg ggccgcccgg agccccgcgc ctgccatgga
     7141 gccgccgggc gacggcgggc gatccacccg ccggcgagcg ccccgcccac cacgcccc
//
LOCUS       DASQQQ010000011        18624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_248648, whole genome shotgun sequence.
ACCESSION   DASQQQ010000011 DASQQQ010000000
VERSION     DASQQQ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 18624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18624
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248648"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VE646_00460"
     CDS             <1..616
                     /locus_tag="VE646_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZD78501.1"
                     /translation="KEALEAVEGSRGSGTVVRLDTLDEASVADAFRQVSETLGPVTGL
                     VNNAGFSRDGLMLKYSMDMFDRTLDTNVRGTFLCTQTALRTMLRARWGRVVNMSSVIA
                     MRGNAGQTAYAASKTALVGLTKSLAREVGPKGITVNAVCPGLVDTEMTSHLSEQARAL
                     FIDQTPLGRTARLEEVAAVIRFLMSDGASYVNGAVIPVDGGLTA"
     gene            676..924
                     /gene="acpP"
                     /locus_tag="VE646_00465"
     CDS             676..924
                     /gene="acpP"
                     /locus_tag="VE646_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017319975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZD78502.1"
                     /translation="MDKEEIDTRVRKVLAEQLAVDETQVVPDARFAEDLNADSLDLVE
                     AVLALEEEWNIEIPEEEMDGVKTVGQAVELVASKLGVS"
     gene            914..2167
                     /gene="fabF"
                     /locus_tag="VE646_00470"
     CDS             914..2167
                     /gene="fabF"
                     /locus_tag="VE646_00470"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HZD78503.1"
                     /translation="MSPSSASPSRRVVVTGIGPVTPIGTGVDEFWTGATAGRNGIRPI
                     SRFDASDLPVKLAGEVQDFDVSRWLDPKEARRTDRFVHFALGSADLAWDDAGKPDVHG
                     DRVAVIFATGIGGIEWLLNQHSVLLEKGPGRVSPFMVPALMANGAAGHIAMRYGFTGP
                     NFCTVSACASGAHAVGEGYRMIRDGLADLAVVGGSEAATLPLTVAAFAQMTALTKNPD
                     PETASRPFDAGRNGFVLSEGACTLILEPQEAAVARGARVYAEVVGYGASDDAYHITAP
                     DPKGSGAALAISRALDDARVEPQAIDYVNAHGTSTPLNDASETAALKAALGEDASRAV
                     AVSSTKSMTGHMLGAAGAVEAAVCALVIHTGMIPPTIHYRTPDPDCDLDYVPNDAREA
                     RVDLAISNSFGFGGQNAVLALAAPA"
     gene            complement(2183..3808)
                     /locus_tag="VE646_00475"
     CDS             complement(2183..3808)
                     /locus_tag="VE646_00475"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZD78504.1"
                     /translation="MALAEAFERIGAFLEDRLPLMHAAGTALAVTDRDEILGVVVRGF
                     ADAASGTPVRPETRFQIGSISKSFTAIVTLQAAEEGLLDLHAPIDRLVPWIEVPQPFG
                     AITPHHLLTHTSGLPIGTEDAPGALAAAWNLRHVRPGFGPGERFWYSNDGYKLVGLVL
                     ERALGRPFGDLVRERILAPLEMGATSPVITNRDRADLATGYATLHDGRPPHRHQPLVP
                     APWAVSGSADGSIVSNVLDMTAYARMLLAAGAPLLSPSGFEALTAPRIQDTGAAGFAY
                     GYGLYVGEQDGRRRIYHTGGMIGYTALLMLDPDDGLGLVMLVNGQGERKPAGRFALRT
                     VRAALTGEPLPPVVHLDPVRTPHAAGYVGRYSDGRRSFEVAARGDRLVLRRNGTETIL
                     EPRLEDEVPTQDLFVAPDPELERSWLWFGRNAEGEVVEAVHGPDRMPREGVTRPEPPP
                     VPERWPGFTGLYRSNDPWIPCFRVVLRGGELWMLLGDDDEPELPLVPIGDEDFRVGRE
                     PWRPSRVAFDRIVEGRAIRAVHDGGSWYRSFED"
     gene            complement(3833..4243)
                     /gene="ndk"
                     /locus_tag="VE646_00480"
     CDS             complement(3833..4243)
                     /gene="ndk"
                     /locus_tag="VE646_00480"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZD78505.1"
                     /translation="MAVESTLLIVKPDGVQRGLVGEVLRRIEAKGLCIADLRMLKIDA
                     ELADRHYAEHRDKPFFQELVGFITSGPVVVARVEGEQAIGVLRTLMGPTNPASAPPGT
                     IRGDLATVITQNIVHGSDSMASAERELALFFPAG"
     gene            complement(4247..5527)
                     /locus_tag="VE646_00485"
     CDS             complement(4247..5527)
                     /locus_tag="VE646_00485"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006542687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HZD78506.1"
                     /translation="MRFSEALAELDARQPERMVPDLSRITALAELLDDPQLTYPTLHI
                     TGTNGKTTTARVVTSLACAHGLTAGLYTSPHLLSVRERIALCGVPISEEEFTETYEHL
                     LPFLRTVDGRGKRVTYFETLTALSFLWFSDKPVSLGVFEVGMGGAWDATNLVAGDVAV
                     LCPIALDHPELGSTVLEVATEKAGIIKPGKVVVAREQPPDALGVIRDRCREYEARLVL
                     EGPDFGIEERRQAVGGQQLLVRGTEASYDDLFVPLVGEHAARNAGAAVVALEAFLERP
                     LSADAVREGLAAASSPGRLEIVGRHPLVVLDGAHNPAGAAALAGALREAFTWERLHLV
                     IAVSANKDIPGIAAALAPLAARAYATRNSSERSAPADQVGRAMAAAGTPTETFGSVEE
                     ALVAAGASAGARDLILVTGSLYTVADARRTSEGS"
     gene            complement(5533..8073)
                     /gene="valS"
                     /locus_tag="VE646_00490"
     CDS             complement(5533..8073)
                     /gene="valS"
                     /locus_tag="VE646_00490"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006539006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HZD78507.1"
                     /translation="MDVPEKPSLEGLEAKWGEWWEATGTYRFDRTQDRGRVYAIDTPP
                     PTASGSLHIGHVMSYTQTDLMARYERMRGLDVFYPMGWDDNGLPTERRVQNYFGIRCD
                     PSLPYDPDFDLTTLERPDDGRPVAVSRGNFIEVCNRLTQEDEKSFEHLFRLLGLSVDW
                     SQTYTTIGEHARRVSQRAFLRQLSRREAYTAAAPTMWDVDFQTAVAQAEIEDREQDGL
                     YHRVAFDRPGGGTVQIETSRPELIPACVALVAHPEDERYRALFGATVLTPLFGVPVPV
                     VAHELAEPDKGTGIAMICTFGDVTDVTWWRDLHLPTRVVIGRDGTIAPPRWGEGGWPS
                     NDVQAARRAQEQLQGLTAKQARRRIAELLRDSGHLLGEPETVRQVVKFYERGERPLEI
                     ITSRQWFVHTLRVRERLLARGRELEWHPGFMGARFASWVEGLNSDWAMSRQRFYGVPF
                     PVWYPVDDRGEPDHQHPIVPDESSLPIDPQEEAPPGYRPEQRGQPGGFVGDPDIMDTW
                     ATSSLTPQIAGHWEDDPVLFSRVFPMDLRPQAHEIIRTWLFYTVLRAELEHGSLPWTD
                     AAISGFVLDPERKKMSKSKGNVVTPLKVFEDHGADAVRYWAASARLGVDATFDEQQMK
                     VGRRLAIKVLNASRFALSMEAEEGPVTEPVDRAMLAVLGGVVEQATEAFDGYDHARAL
                     EVIERFFWGFCDDFLELVKQRAYGARGPAAASSAVSALRVALDAQLRMLAPFLPYVTE
                     EVWSWWRSGSIHHAAWPRRDELAAASAGEPDVFRVAAEVLTAVRKEKALNKLSLRTPA
                     EVVVVRDTPERLGALALAEDDLREAGNIGTLQAAEADVFAVDVVLAPG"
     gene            8246..8971
                     /locus_tag="VE646_00495"
     CDS             8246..8971
                     /locus_tag="VE646_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011738962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD78508.1"
                     /translation="MEQAVRGARLRLEGVVKTYGSGQIAVHALRHVDLEVSSGEFVVL
                     LGPSGSGKTTLLNVVGGIEPPTSGRVVVDGVDLNSLDAEGRTVYRRDHVGFVFQFFNL
                     IPTLTARENVGLIAELTGSADGERVDRVLGEVGLGERVDHFPGALSGGEQQRVAIARA
                     LAKDPPLLLCDEPTGALDLETGRTVLDLLRRLNAQRGCTVLLVTHNSAIAEMADRVVR
                     MRSGSLEEDRRNPTPVAARALRW"
     gene            8965..11310
                     /locus_tag="VE646_00500"
     CDS             8965..11310
                     /locus_tag="VE646_00500"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD78509.1"
                     /translation="MVRLLRVKRRRDLRRQAAQFGAVVATLFLGVALFGATSDAYRNL
                     NASYQMLYRDLRFADLTVTGGPVERLSGEARAAGALATVRTVADVPFEVNGSKLLGRV
                     VGMPAGGQPSVDRVNVLSGSYLDPSEPAGVLVEQHMASHFHLVPGDTVRILGPQGWTE
                     ARVLGVAASPEYLWPARSRQEVLTPPGDFGVLFAPQGLARAVASPDTPTQVAIYGASP
                     SFLSTWQRQARTGGALDIQTRAEQPSNAALQEDVSGFGELALLFPILFLSAAAMATFV
                     LLGRLVRSQRAEIGMLTANGFGRREIFRTYLGYGVLAGAVGGVLGAAAGVALGGWITH
                     LYTSAISVPITVVRVTPSTPIIGVGIALAAGAIAATAPALAAARVTPAEAMGGISPAA
                     SGGRSLVERLVPPLRRLPTRWKLAIRDIGRSRRRTISTMVGVILALVLVLVSWGMLDT
                     TDILLSRQFDVVQRQDAQLYFSGGTASNTELRAMKGVPGVAAVEAAGEMPVSISYDDR
                     SYATTLLAMQPGTSMHTFLLTGGGRGRLSGDGILAGSALETQLGVHVGDVVRLNSPAL
                     SSLIELPIAGFVQEPLGTFAYAPLPAIERTTHRTLANTALVRFSAGANSKATLDRLRA
                     LPSVAAVVDSRGLVSAAGSLMGLFYVFVGIMLLFGAVMSFALMFNTMSANVAERSTEM
                     ATLRASGVGRRTISGILTRENLVVTALGIVPGLVIGYWLSAVFMSSFSSDLFSFALQM
                     RSTTLLASALAMVLVALVSQWPGVRAVGRLDVATVVRERSV"
     gene            11554..14907
                     /locus_tag="VE646_00505"
     CDS             11554..14907
                     /locus_tag="VE646_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZD78510.1"
                     /translation="MTILFADVTGSTSIGERLDPERLREVMDAYFAAMREEIEAEGGT
                     VEKFIGDVVMAVFGVPVAHEDDAARALRAGLRMQRRLPELNAQLQADHEVSLQIRIGV
                     HTGEVLATTEPRPDEPMATGDAVNAASRLQSAARPGEVLASERTIRSIRGFRTEDAGR
                     LELKGKSEPVHAFRVLEETPAAPERGVAGLHAPMVGREEELELLRSLFGRVARERRPH
                     LVTLYGDAGVGKSRLTREFLETLDGRAQVVRGRCPPYGEGVTFWPLAEILKARAGFLD
                     TDPPEVALERIRSAGRALLDEQVTTDPARSTAALAFTVGAEDPDYPFSRMDPKDVRAQ
                     VHASWRSFFSALAQERPVVVVIEDIHWADPALLDLLEHLSERAQGPLLFVCPSRPELT
                     SHRPGWGGGRRNHSSVALDPLTPDQSDRLVGFLLAVDDLSPTVRRRILERAEGNPFFL
                     EEIVRHLIDEGFLVRDGGRWRTAPGIDDVEIPDTVQRVLAARIDLLSAEHKRVLQAAA
                     VVGRIFWPGPLEELVSGADITETLTALEARELILSRLSSSIAGAPEFMFKHILTRDVA
                     YESLPRRERSPAHAAIAGWIERTAGPRAREFAELLAYHYATAVTGSSNPVDEELRRRA
                     FEHLVTASAQARGRQVLKKAERFAEQALALARDDAERATALEALGDACFAGYQGDLGW
                     RYFGDAVRAREAAAVGHPDRKLAYLCARAAEFPTRWPGSMRQIPGPEQVEPLLDLGMR
                     HVPQGDSEERARLQACRASWPFAFPQLELSREEGLGCERAGMEAAEMALRLARPDIAS
                     GAFDAAAGFAISLGSYRRALDIEERRLELSEGIGDFELGDLYAMLAWGNYELGAYERG
                     LRMADQGLTLIGGREPTVGVHLIGWRAALRARLGDWDGATADLGEVRSLLDDRRDDPP
                     YFASHAYAVAATIFERRGESVESDRLTGLLLPLAGHFSARLFGWLVILLVERGDLEKA
                     GELMEVLPPSWRVHAATVFEARCELVAAAGRWGEVPELLVQARAYADASGSRSLPAFA
                     DRLEGRLTLATGEASEAARLLSRTVDDFRDLQTPYEEAVTRMDLARSLMLSGRREDAL
                     LTLDRAQATLEDLGAAKRLVSVRDLRAEA"
     gene            complement(14957..16216)
                     /gene="clpX"
                     /locus_tag="VE646_00510"
     CDS             complement(14957..16216)
                     /gene="clpX"
                     /locus_tag="VE646_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HZD78511.1"
                     /translation="MGRLGDSDLLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNEI
                     IEEELSETSELKLDELPKPQEIYAFLDDYVIGQEQAKKVLSVAVYNHYKRIQVGAKDG
                     EVELQKSNILILGPTGCGKTLLAQTLAKMLNVPFAIADATALTEAGYVGEDVENILLK
                     LIQAADYDVKKAETGIIYIDEVDKIARKSENPSITRDVSGEGVQQALLKILEGTTASV
                     PPQGGRKHPHQEFIQIDTTNILFICGGAFAGLEKIIESRVGSRGVGFGADVRRVDEKD
                     LGVILGRVLPEDLLKFGLIPEFVGRLPVLTNVHNLDKQALVDILTKPRNALVKQYRRF
                     FEFDGVDLEFADDALQAIADQAILRGTGARGLRAIMEEVLLNVMYELPSTEGVAKCVI
                     DHDVVRSGVNPTLVPRQEPRVRKERSA"
     gene            complement(16291..16929)
                     /locus_tag="VE646_00515"
     CDS             complement(16291..16929)
                     /locus_tag="VE646_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HZD78512.1"
                     /translation="MSELTNYLVPVVVEQTSRGERSFDIYSRLLKERIVFLGTPIDDN
                     VGNLIMAQLLHLESEDPEKDINLYINSPGGDITALFAIYDTMQYIKPDVSTIVMGQAA
                     SAAAVLLLAGTKGKRYALPHSRVLLHQPHGGAQGQAVDIEIQAKEITRYRKLLEELIA
                     KHTGQSLDKVAKDTDRDYILTAEEAVEYGVVDEVISSRKAHPELAAAAAGGS"
     gene            complement(16953..18305)
                     /gene="tig"
                     /locus_tag="VE646_00520"
     CDS             complement(16953..18305)
                     /gene="tig"
                     /locus_tag="VE646_00520"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HZD78513.1"
                     /translation="MQTTVEETDKHIVKLTVEIPAERLGKDLDRTYRRVAQQVRVPGF
                     RKGKVPRRIIDTQIGRDTVLSEFLEETVPSYYREAVRDNDLAPISEPDISLEQVEEGK
                     PLIFTATMEVRPRLKLEERDYKGLEVRRPSTEVAESEVDEMIDGLRERFAELETAAHP
                     ARKGDYVVIDLRATIHDQEVPEATRPDYLYEVGSEGFTGKLDEELEGKRAGEILRFNA
                     VLDERFGERSGQEVSFQVLVKEVKGKKLPAADDAFAKTASEFDTLDELKESLREQIAV
                     NKERNADAEVRDLVLQKLVDSLDLDLPDTLVDEETEHRVQHARERAERAGMRLEQLLE
                     AQGFDELRFRSDARAHALRAIKADLVLESVARNEELEVTAEELGREVASLATALGREP
                     KEVAKTLEASGQVVALASDIIRSKALDLLVEQAEITSEGEGRASTPAEAEAEEASEPP
                     "
     gene            18387..>18624
                     /locus_tag="VE646_00525"
     CDS             18387..>18624
                     /locus_tag="VE646_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HZD78514.1"
                     /translation="MLEEELAFARQVADEADRIALSFFGSDPAVRLKPDRTPVTEADE
                     RIEAMVRARLAQRFPQDAILGEEEGSLGTGDRRWV"
BASE COUNT         2598 a         6586 c         6542 g         2898 t
ORIGIN      
        1 gaaggaggca ctggaggccg tggagggctc ccgcggctcc gggaccgtgg tccggctcga
       61 cacgttggac gaggcctcgg tggccgacgc cttccgtcag gtgtccgaga ccctgggccc
      121 cgtgaccggt ctggtgaaca acgcgggctt ctcccgagac gggctcatgc tcaagtactc
      181 gatggacatg ttcgaccgca ccctagacac gaacgtccgc ggcaccttcc tgtgcaccca
      241 aacggccctg cggacgatgc tccgagctcg gtggggacgg gtggtgaaca tgtcctcggt
      301 gatcgcgatg cggggcaacg ccggccagac ggcctacgcg gcatcgaaga cggccctggt
      361 ggggctcacg aagtccctgg cgcgcgaggt aggacccaag ggcatcaccg tgaacgccgt
      421 gtgcccgggc ctggtggata ccgagatgac atcccacctc agcgagcagg cccgagccct
      481 cttcatcgac cagacgccgc tcgggcgaac cgcccggctg gaggaggtgg cggccgtgat
      541 ccggttcctg atgtcggatg gcgcctcgta cgtcaacggc gcggtgatcc ccgtggacgg
      601 cgggctcacg gcatagtcgc caggccccgg agaccctaga gtttgcggca acctcaggcg
      661 agaacgggag gaaggatgga caaggaggag atcgacaccc gggtccgcaa ggtcctggcg
      721 gagcagctgg ccgtggacga gacgcaggtc gtcccggacg cccggttcgc cgaggacctg
      781 aacgcggact cgctggacct ggtggaagcc gtcctggccc tcgaggagga gtggaacatc
      841 gagatccccg aggaggagat ggacggggtc aagaccgtgg ggcaggccgt ggagctcgtc
      901 gcctccaagc tcggtgtctc ctagctcggc gtctcccagc cggcgggtcg tcgtcaccgg
      961 gatcggtccg gtcacgccga tcggaaccgg cgtggacgag ttctggaccg gtgccacggc
     1021 gggacggaac gggatccgac cgatctcgcg gttcgacgcc tcggacctgc cggtgaagct
     1081 ggccggggag gtccaggact tcgacgtctc tcgatggctg gaccccaagg aggcgcgccg
     1141 aaccgaccgg ttcgtgcact tcgccctggg ctccgccgac ctcgcctggg acgacgccgg
     1201 gaagccagac gtgcacggcg accgggtggc cgtgatcttc gccaccggga tcgggggcat
     1261 cgaatggctg ctcaaccagc attcggttct gttggagaag ggacccggcc gggtgagccc
     1321 gttcatggtc ccggcactga tggccaacgg ggcggcgggg cacatcgcga tgcggtacgg
     1381 cttcacgggg ccgaacttct gcacggtgtc ggcgtgcgcc tcgggtgccc atgcggtggg
     1441 cgagggctac cggatgatcc gggacgggct cgcggacctg gcggtggtgg gcggctccga
     1501 ggcagccacc ctgcccctca cggtggccgc cttcgcccag atgaccgccc tcaccaagaa
     1561 cccggacccg gagaccgcca gccgtccctt cgatgccggg cgcaacggct tcgtcctctc
     1621 cgagggcgca tgcacgctga tcctggagcc gcaggaggcc gctgtggccc gcggcgcgcg
     1681 ggtctacgcg gaggtcgtcg gctacggcgc ctcggacgac gcgtaccaca tcacggcccc
     1741 cgatcccaag ggctcggggg cggcgctcgc gatcagtcgt gcgctcgacg atgcccgcgt
     1801 cgagccccag gccatcgact acgtcaacgc ccacggcacc tccaccccgc tgaacgatgc
     1861 gtccgagacc gcggccctca aggccgcgct cggcgaggac gcgtcgcgcg cggtggccgt
     1921 ctcctccacg aagtccatga cgggccacat gctgggtgcc gccggagccg tggaggcagc
     1981 cgtctgcgcc ctggtcatcc acacggggat gatcccgccc acgatccact accggacgcc
     2041 cgatcccgac tgcgacctgg actacgtccc caacgatgcg cgggaggctc gggtggacct
     2101 ggcgatctcg aactccttcg gcttcggagg ccagaacgcg gtgctggccc tcgccgcgcc
     2161 cgcctgatcc cccgggcggt cctcagtcct cgaacgagcg gtaccacgag ccgccgtcgt
     2221 ggacggcccg gatcgctcgc ccctccacga tccggtcgaa cgccacccgg ctggggcgcc
     2281 agggctctcg ccccacgcgg aaatcctcgt ccccgatggg caccagcggc agctccggct
     2341 cgtcgtcgtc gcccagcagc atccacagct caccgccccg gagcacgacc cggaagcagg
     2401 ggatccacgg gtcgttcgag cggtagagac cggtgaagcc cggccagcgc tcgggcaccg
     2461 gcggcggctc cggcctcgtc acgccctctc ggggcatgcg atcggggccg tggacggcct
     2521 ccaccacctc gccctccgcg ttgcggccga accacagcca ggagcgctcg agctccgggt
     2581 cgggggccac gaacaggtct tgggtgggga cctcgtcctc caagcgcggc tcgaggatgg
     2641 tctcggtccc gttccgccgc aggaccaggc gatcgccccg agccgccacc tcgaacgacc
     2701 gccggccgtc cgagtagcgg cccacgtacc ccgccgcgtg cggtgtgcgc accgggtcca
     2761 ggtggaccac cggcgggagc ggctcgccgg tcagggcggc ccggaccgtc cgtagggcga
     2821 agcgtccggc cggcttgcgc tccccctggc cgttcaccag catgaccagc cccaacccgt
     2881 cgtccggatc gagcatgagc agggccgtgt acccgatcat cccgcccgtg tgatagatgc
     2941 ggcgccgtcc gtcctgctcg cccacgtaga ggccgtagcc gtatgcgaac ccggccgcgc
     3001 ccgtgtcctg gatgcgagga gccgtgagcg cctcgaaccc ggacggggac agcaggggtg
     3061 cgcccgccgc cagcagcatc cgcgcgtacg cggtcatatc caggacgttg gagacgatgc
     3121 tgccgtcggc cgagccgctc acggcccagg gggcggggac cagcggttgg tgccggtggg
     3181 gcggacggcc gtcgtggagg gtcgcgtagc cggtggcgag gtccgcgcga tcccggttcg
     3241 tgatgacggg gctcgtggcc cccatctcga gcggcgcgag gatccgctcc cgcaccaggt
     3301 ccccgaacgg ccttcccagg gcccgctcca ggaccagacc cacgagcttg tagccgtcgt
     3361 tcgagtacca gaaccgctcc ccggggccga accccggccg gacgtggcgg aggttccagg
     3421 ccgcggccag cgctccgggc gcgtcctcgg tcccgatggg gagtccggac gtgtgcgtca
     3481 acaggtggtg cggggtgatg gccccgaagg gctggggcac ctcgatccac ggcacgagcc
     3541 gatcgatggg cgcgtgcaga tccaggagcc cctcctccgc ggcttggagg gtcacgatgg
     3601 ccgtgaacga cttgctgatg gagccgatct ggaaccgggt ctcgggtcga acgggtgtgc
     3661 cagaggccgc atccgcaaac ccgcgcacca ccacgccgag gatctcgtca cggtccgtga
     3721 cggccagcgc cgtcccggct gcgtgcatca gcggcagccg atcctccagg aacgccccga
     3781 tgcgctcgaa ggcctccgcc agcgccacga ccgccccagc ccctcggccg cctcagccgg
     3841 cggggaagaa cagggccagc tcccgctcgg ccgaggccat cgagtcgctg ccgtgcacga
     3901 tgttctgggt gatcaccgtg gccaggtcgc cgcggatcgt cccggggggg gccgacgccg
     3961 ggttggtggg acccatgagg gtgcgcagga caccgatggc ctgctccccc tccacgcgcg
     4021 ccaccaccac cggccccgag gtgatgaagc ccacgagctc ctggaagaag ggcttgtccc
     4081 ggtgctcagc gtagtggcgg tcggccagct cggcgtcgat cttgagcatc cggagatccg
     4141 cgatgcacaa gcccttggcc tcgatccgac gcaggacctc tcccaccaga ccacgctgca
     4201 ccccgtcggg cttcacgatg agcagggtcg attcgacggc cacggatcaa gatccttccg
     4261 aggttcggcg ggcgtccgcg acagtataga gagagcccgt caccaggatc aggtcccgtg
     4321 ctccggcgct ggccccggcc gccaccaggg cctcctccac cgagccgaag gtctcggtgg
     4381 gcgtaccggc cgccgccatc gcccggccca cctggtcggc cggggccgag cgctcgctgc
     4441 tgttgcgggt ggcgtacgcg cgggcggcca gcggtgccag cgcggcagcg atccccggga
     4501 tgtctttgtt cgcggagacg gcgatcacca gatgcagccg ctcccacgtg aacgcttccc
     4561 gcagggcccc ggccagcgcc gcggccccgg ccgggttgtg tgccccgtcg agcacgacca
     4621 gcgggtgccg gcccacgatc tcgagccgcc ccggggacga ggcggccgcg agcccctccc
     4681 ggaccgcatc ggccgagagg gggcgctcca ggaaggcttc cagcgcaacg acagcagccc
     4741 cggcgttccg agcggcatgc tcacccacga ggggcacgaa gaggtcgtcg tagctcgcct
     4801 cggtcccccg gacgagtagc tgctgtccgc ccaccgcctg ccgacgctcc tcgatcccga
     4861 aatcgggtcc ctccaggacc aggcgcgcct cgtactcgcg gcaccggtcg cggatcacgc
     4921 ccagggcgtc gggaggctgt tcgcgcgcga ccaccacctt gcccggcttg atgatgcccg
     4981 ccttctccgt ggccacctcg agcaccgtgg agcccagctc cggatgatcg agggcgatcg
     5041 ggcacaggac ggccacgtcg ccggcgacca ggttggtggc atcccaggca ccccccatgc
     5101 ccacctcgaa cacaccgagc gagacgggct tgtccgagaa ccacaggaac gacagcgccg
     5161 tcagggtctc gaagtaggtg acgcgcttcc cccgcccgtc caccgtgcgg aggaacggca
     5221 gcaggtgctc gtaggtctcc gtgaactcct cctcggagat cggcacgccg cacagggcga
     5281 tccgctccct caccgacagc aggtgtggcg aggtgtacag gccggccgtg agaccgtggg
     5341 cacaggccag cgaggtcacc acccgggccg tggtggtctt gccgttcgtg ccggtgatgt
     5401 ggagcgtggg gtacgtgagc tggggatcgt cgagcagctc ggccagtgcg gtgatccggc
     5461 tcaggtcggg gaccatgcgc tcgggttgcc gggcgtcgag ctccgccagg gcttcggaga
     5521 atcgcatcgg gctcagcccg gggccaggac gacgtccacg gcgaacacgt cggcctccgc
     5581 cgcctggagc gtgccgatgt tcccggcctc ccgcaggtcg tcctcggcca gggcgagcgc
     5641 cccgagccgc tccggcgtgt cccgcacgac caccacctcc gccggtgtcc ggagggagag
     5701 cttgttcagg gccttctcct tgcggaccgc ggtgaggacc tcggcggcca cgcggaacac
     5761 gtcgggctct ccggcggagg cggcggccag ctcgtcccgc cgcggccagg cggcgtgatg
     5821 gatcgatccc gaacgccacc atgaccagac ctcctcggtg acgtagggga ggaagggggc
     5881 cagcatccgg agctgggcgt cgagcgcgac gcgcagggcc gacacggccg aggaggcggc
     5941 cgccggacct cgggcgccgt aggcgcgctg cttcaccagc tccaggaagt cgtcgcagaa
     6001 gccccagaag aaccgctcga tgacctccag cgcgcgggcg tggtcatagc cgtcgaacgc
     6061 ctcggtggcc tgctccacca cacctccgag aaccgccagc atggcccggt ccaccggctc
     6121 ggtcaccggt ccctcctccg cctccatcga cagcgcgaag cgtgaggcgt tcagcacctt
     6181 gatcgccagt cggcgtccca ccttcatctg ctgctcgtcg aacgtggcgt ccaccccgag
     6241 ccgcgcgctg gccgcccagt agcggacggc gtcggcgccg tggtcttcga acaccttcag
     6301 cggcgtgacc acgttgccct tggacttgga catcttcttg cgctccgggt ccaggacgaa
     6361 gccgctgatc gcggcatcgg tccagggcag ggacccgtgc tcgagctcgg cccggagcac
     6421 cgtgtagaac agccaggtcc ggatgatctc gtgcgcctgg ggacgcaggt ccatggggaa
     6481 cacccgcgag aacaggaccg ggtcgtcctc ccagtgcccg gcgatctggg gggtgaggga
     6541 cgaggtggcc cacgtatcca tgatgtcggg gtcccccacg aatccgccgg gctgcccgcg
     6601 ttgctccggc cggtaccccg ggggcgcctc ctcctgagga tcgatcggga gcgaggactc
     6661 gtcgggcacg atgggatgtt ggtgatcggg ctcccctcgg tcgtccaccg ggtaccagac
     6721 cgggaacggc accccgtaga agcgctgccg gctcatcgcc cagtcgctgt tcagtccctc
     6781 cacccacgag gcgaacctcg cgcccatgaa cccggggtgc cactcgagct cgcggccccg
     6841 cgccaagagc cgttcccgga cccgcagggt gtgcacgaac cactggcggc tggtgatgat
     6901 ctcgagggga cgctcgcccc gctcgtagaa cttcacgacc tggcgcaccg tctccggctc
     6961 accgagcaga tggcccgagt cccgcaggag ctccgcgatc ctgcgtcgtg cctgcttcgc
     7021 ggtcaggccc tgcagctgct cctgcgcccg tcgggccgcc tgcacgtcgt tcgatggcca
     7081 accgccctca ccccaccgag gcggggcgat cgtcccgtca cgaccgatca cgacccgggt
     7141 gggcaggtgc aggtcccgcc accacgtcac gtcggtcacg tctccgaagg tgcagatcat
     7201 cgcgatcccg gtgcctttgt ccggctccgc cagctcgtgc gccaccaccg gcacggggac
     7261 cccgaacagc ggcgtgagca cggttgcccc gaacagggct cggtagcgct cgtcctcggg
     7321 atgggccacc agggcgacgc aggccgggat cagctcgggg cgggaggtct cgatctgcac
     7381 cgtgccgccc ccgggccgat cgaacgcgac ccgatggtac aggccgtcct gctcccgatc
     7441 ctcgatctcg gcctgggcga cggcggtctg gaagtccaca tcccacatcg tgggggccgc
     7501 cgccgtgtag gcctctcggc gggacagctg gcgaaggaac gcccgctggg acacccgccg
     7561 agcatgctcc ccgatcgtcg tgtaggtctg ggaccagtcc acggacaggc cgaggagccg
     7621 gaagaggtgc tcgaagctct tctcgtcctc ctgggtgaga cggttgcaca cctcgatgaa
     7681 gttgcctcga gagacggcga ccggccgccc atcgtcggga cgctccagcg tggtcaggtc
     7741 gaaatccggg tcgtagggca aggaaggatc gcaccggatc ccgaagtagt tctggacacg
     7801 gcgttccgtg ggcagcccgt tgtcgtccca ccccatgggg tagaagacgt cgagcccgcg
     7861 catccgttcg tacctggcca tcaggtcggt ctgcgtgtac gacatcacat ggccgatgtg
     7921 cagggagccg ctcgcggtgg gcggtggggt gtcgatcgcg tacacgcgtc cccggtcctg
     7981 cgtgcgatcg aagcgatagg tcccggtggc ctcccaccac tccccccact tggcctccag
     8041 gccctcgagg gacggcttct ccggaacgtc catggcgccg agtgtacggg acccatccgg
     8101 tccccgatcg accccaacgg gacttggtct atggttcggt acggtcgacc ccaggcccga
     8161 tgtcatgcag agggcaggcc cgatcggcgt gatgttccgg ccgggggatc gtcgcgctgg
     8221 agcacccgat gagggaagga gcgacatgga gcaggcggtt cgcggggccc ggctgaggct
     8281 cgagggcgtg gtcaagacct acggcagcgg ccagatcgcg gtgcacgcgc tccgccacgt
     8341 cgatctggag gtgtcctcgg gggagttcgt ggtcctgctg ggtccgagcg gatcgggcaa
     8401 gaccaccctg ctgaacgtgg tgggggggat cgagccaccg acctcggggc gagtggtggt
     8461 ggacggcgtc gatctgaact cgctggacgc cgaaggacgt accgtctacc gccgcgacca
     8521 cgtgggcttc gtgttccagt tcttcaacct gatccccacg ctgacggccc gggagaacgt
     8581 cggcttgatc gccgagctca ccggctcggc cgacggggaa cgggtcgacc gcgtgctggg
     8641 cgaggttggg ctcggggagc gggtggatca cttccccggg gcgctgtccg ggggtgagca
     8701 gcagcgcgtg gcgatcgccc gggcgctggc caaggacccg ccgctcctgc tgtgcgacga
     8761 gcctaccggc gcgctcgacc tggagaccgg ccgcacggtc ctggacctgc tccgccgcct
     8821 caacgcgcag cgcggctgca ccgtgctgct ggtgacgcac aactcggcca tcgccgagat
     8881 ggccgatcgg gtggtccgga tgcgatcggg atcgctcgag gaggaccgca ggaaccccac
     8941 cccggtggcg gcgagggccc tgcggtggtg aggctgctgc gggtcaagcg gcggcgggac
     9001 ctgcgacggc aggcagcgca gttcggcgcc gtggtcgcca cgctgttcct cggcgtggcc
     9061 ctgttcggtg cgacctccga tgcctaccgc aacctcaacg cgtcctacca aatgctctac
     9121 cgtgacctgc ggttcgccga cctcaccgtg accggagggc cggtcgaacg cttgagcggt
     9181 gaggcccggg cggcgggggc gctcgccacg gtgcgcaccg tggcggacgt cccgttcgag
     9241 gtgaacggct cgaagctgct gggccgcgtg gtggggatgc cggcaggcgg ccagccgtcc
     9301 gtcgaccggg tgaacgtcct ctcgggcagc tacctcgatc cttctgagcc ggccggcgtc
     9361 ctggtcgagc agcacatggc ctcgcacttc cacctggtcc ccggcgacac cgtcaggatc
     9421 ctcggccccc aggggtggac cgaggcgcgg gtgctgggcg tggccgcctc ccccgagtac
     9481 ctgtggccgg cgcgcagccg gcaggaggtc ctcaccccgc cgggcgactt cggcgtcctg
     9541 ttcgcgcccc aggggctggc ccgcgcggtc gcatcccccg acaccccgac ccaggtcgcg
     9601 atctacgggg cgagcccctc cttcctgtcg acctggcaac gacaggcccg caccggcggc
     9661 gccctcgaca tccagacccg cgccgagcag ccgtcgaacg ccgctctcca ggaagacgtg
     9721 agcggcttcg gcgagctggc cctgctgttc cccatcctct tcctgtcggc ggccgccatg
     9781 gccaccttcg tcctgctcgg tcgcttggtc cgatcgcagc gggcagagat cggcatgctc
     9841 acggccaacg ggttcggccg acgcgagatc ttccgcacgt acctcggcta cggcgtcctg
     9901 gcgggcgccg tgggcggggt gctgggcgcg gcggccggcg tcgcgctggg cggctggatc
     9961 acgcacctgt acacctcggc gatctccgtc ccgatcaccg tggtccgcgt caccccctcc
    10021 acgccgatca tcggggtggg gatcgcgctg gccgcggggg cgatcgcggc gaccgcgccg
    10081 gcgctggccg cggcccgggt cacgccggcc gaggcgatgg gcgggatctc cccggccgcc
    10141 tcgggcggac ggagcctggt ggagcgtctg gtcccgcccc tccggcggct ccccacccgc
    10201 tggaagctcg cgatcaggga catcggccgg agtcggcggc ggacgatctc gaccatggtg
    10261 ggggtgatcc tcgccctggt cctcgtgctg gtctcgtggg ggatgctcga caccaccgac
    10321 atcctcctgt cccggcagtt cgacgtggtg cagcgtcaag atgcccagct ctacttctcc
    10381 ggcggcaccg catccaacac cgagctcagg gccatgaagg gggtcccggg ggtggcggcc
    10441 gtcgaggccg ccggcgagat gccggtctcg atctcctatg acgaccgtag ctacgcgacc
    10501 accctcctgg ccatgcagcc gggcacgtcc atgcacacct tcctgctcac cgggggcggc
    10561 cggggccgcc tctcgggcga cgggatcctc gcggggagcg ccctggagac ccagctcggg
    10621 gttcacgttg gagacgtggt gcggctgaac tcgcccgccc tgagctcgct catcgagctg
    10681 ccgatcgccg ggttcgtcca ggagccgttg gggaccttcg cgtacgctcc ccttccggcg
    10741 atcgagcgca cgacccatcg gacgctggcg aacacggccc tggtgcgctt ctcggccggg
    10801 gccaactcga aggcgacgtt ggaccgactc cgcgccctgc cgtccgtcgc cgcggtcgtg
    10861 gactcgaggg gcctggtctc ggccgccggg tccctgatgg ggctgttcta cgtcttcgtg
    10921 gggatcatgc tcctgttcgg cgccgtcatg tccttcgcgc tgatgttcaa cacgatgtcg
    10981 gcgaacgtgg ccgagcgctc caccgagatg gccaccctgc gcgcctccgg cgttgggcgc
    11041 aggacgatct cgggcatcct gacgcgggag aacctggtgg tgaccgcgct gggcatcgtg
    11101 ccggggctgg tgatcggcta ctggctctcg gcggtgttca tgtcgtcgtt cagcagcgac
    11161 ctgttctcct tcgccctgca gatgcgatcc acgaccctgc tcgcctccgc cctggccatg
    11221 gtcctggtcg ccctggtctc ccagtggcca ggcgtccggg cggtgggaag gctcgacgtg
    11281 gccacggtgg tgcgggaacg gtcggtgtga gcgcggtcgc gccctcctga cccgggcggc
    11341 ccatctctct acactcgccc catgccctcg tgtgcccggt gcggatccca gaacgatccc
    11401 gccgcccggt tctgtgcggc gtgcggtgcg ccgctcgcct cggcctgctc ggcgtgcggg
    11461 gccacccttc ctcccgacgc tcggttctgt ccggcctgcg ggcatccggt gtccgggacc
    11521 cccgcgccgc ccccggggca ggagcggaag ctggtgacga tcctgttcgc ggacgtcacc
    11581 gggtccacga gcatcggcga acggctggat cccgaacgtc tgcgagaggt gatggacgcc
    11641 tacttcgccg cgatgcggga ggagatcgag gccgaggggg ggacggtcga gaagttcatc
    11701 ggggacgtgg tcatggccgt gttcggggtc ccggtcgccc acgaggacga cgccgcccgg
    11761 gccctgcgag cgggcctgcg gatgcagcgt cgcctgcccg agctcaacgc gcagctccag
    11821 gcggatcacg aggtgagcct gcagatccgg atcggtgtgc acaccggaga ggtcctggcg
    11881 accacggaac cccgacccga cgagccgatg gccacgggcg atgccgtcaa cgcggcctcc
    11941 cgcctccagt cggccgcccg gcccggggag gtgctcgcat ccgagcgcac gatccgctcg
    12001 atccgcggct tccgcacgga ggacgccggt cggctggaac tgaaggggaa gtccgaaccg
    12061 gtccatgcgt tccgcgtact cgaggagacg ccggcggccc ccgagcgcgg tgtggcgggg
    12121 ctgcacgcgc cgatggtcgg acgggaagag gagctcgagc tgctgcggag cctgttcggc
    12181 cgggtggctc gcgaacgccg gccccacctg gtcaccctct acggcgatgc cggcgtgggc
    12241 aagagccggc tcacgcggga gttcctggag accttggacg gtcgggccca ggtggtgcgg
    12301 gggcgctgcc caccctacgg cgagggggtg acgttctggc cgctcgcgga gatcctgaag
    12361 gcgcgggccg ggttcttgga caccgatccg ccggaggtgg ccctggaacg gatccgatcg
    12421 gccggcaggg cgctgctcga cgagcaggtc accacggacc cggcacggag cacggcggcg
    12481 ctggccttca ccgtcggggc ggaggacccc gactacccgt tcagccggat ggatcccaag
    12541 gacgtgcggg cgcaggtgca cgcctcctgg cgctccttct tctcggcgct ggctcaggag
    12601 cgccccgtcg tcgtcgtgat cgaagacatc cactgggccg atcccgccct cctggacctg
    12661 ctggagcacc tctccgaacg tgcccaggga ccgctcctgt tcgtctgtcc ttcccgcccc
    12721 gagctcacct cgcatcgtcc cggatggggg ggcggccgcc gcaaccactc gtcagtggcc
    12781 ctggacccgc tgacgcccga tcagtccgac cggctggtgg ggttcctcct cgccgtcgac
    12841 gacctctctc ccaccgtccg gcgccggatc ctggagcggg ccgagggcaa cccgttcttc
    12901 ctggaggaga tcgtccgtca cctgatcgac gagggtttcc tggtccgtga cgggggccgc
    12961 tggcgcacgg cgcccgggat cgacgacgtg gagatccccg acacggtgca gcgcgtgctg
    13021 gcggcccgga tcgacctgct gtctgcggag cacaagcggg tcctgcaggc tgcggccgtg
    13081 gtgggacgga tcttctggcc cggcccgctc gaggagctgg tgagcggcgc ggacatcacc
    13141 gagaccctga ccgcgctcga ggcccgcgag ctcatccttt cccggctgag ctcctcgatc
    13201 gccggcgcgc cggagttcat gttcaagcac atcctcaccc gggatgtcgc ctacgagagc
    13261 ctcccccgcc gagaacggtc ccccgcccac gcggccatcg ccggctggat cgagcgcacg
    13321 gccgggccgc gagcccggga gttcgcggag ctcctcgcct accactacgc gaccgctgtg
    13381 accggatcct cgaaccccgt cgacgaggag ctgcgccgga gggcgttcga gcatctggtc
    13441 accgcctcgg cccaggcccg tggacggcag gtgctgaaga aggccgagcg gttcgcggag
    13501 caggccctcg cgctggctcg agacgacgcc gagcgggcca cggccctgga ggccctgggg
    13561 gacgcctgct tcgccgggta tcagggcgac ctgggctggc ggtacttcgg ggacgcggtg
    13621 cgggctcggg aggccgcggc cgtgggtcac cccgaccgca agctcgccta cctctgtgcc
    13681 cgcgcggcgg agttcccgac caggtggccc ggttcgatgc gccagatccc ggggccggag
    13741 caggtcgagc cgctgctgga cctcgggatg cggcacgtac cgcagggcga cagcgaggaa
    13801 cgggccaggc tccaggcgtg ccgcgcgagc tggcccttcg ccttccctca gctggagctg
    13861 agccgcgagg agggactcgg gtgcgaacgg gccggcatgg aggcggccga gatggcgctt
    13921 cgactggccc gacccgatat cgcctccggg gccttcgatg ccgcggccgg cttcgccatc
    13981 tcgctcggat cctaccggcg cgccctcgac atcgaggagc ggcggctgga gctctccgag
    14041 gggatcggcg acttcgagct cggcgacctg tacgcgatgc tggcctgggg caactacgag
    14101 ctcggggcct acgagcgggg cttgcggatg gcggatcagg gactgacgct gatcgggggg
    14161 cgggagccca cggtcggggt ccacctgatc ggctggcgcg ccgcgttgcg ggctcgactg
    14221 ggcgattggg acggggcgac ggccgacctg ggggaggtcc gttcgctgct cgacgatcgg
    14281 cgggacgatc caccgtactt cgcctcgcac gcctacgccg tcgcggcgac gatcttcgag
    14341 cgccgcggcg agtcggtgga gagcgaccgg ttgaccgggc tgctgctccc cctcgcgggc
    14401 cacttctccg cccgcctgtt cggctggctg gtgatcctgc tggtcgagcg gggcgacctg
    14461 gagaaggccg gcgagctgat ggaggttctg cctcccagct ggcgggtcca cgcggccacg
    14521 gtcttcgagg cccggtgcga gctcgtggcc gccgctggtc gatggggcga ggttcccgag
    14581 ctgctggtcc aggcccgggc gtacgcggat gcgtccggct cgcggtcgct gccggcgttc
    14641 gcagatcgtt tggaggggcg cctgaccctg gcgaccgggg aagcctccga ggccgcgcgg
    14701 ctcctctcgc ggacggtgga cgacttccgt gacctgcaga ccccgtacga agaagccgtc
    14761 acccggatgg acctggctcg ttctctgatg ctgtccggac ggcgcgagga tgccctgctc
    14821 accctggatc gggcccaggc gaccctcgag gatctggggg ccgcgaagag gttggtgagc
    14881 gtccgcgacc tccgggcgga ggcctaggcg cggggtcgct gctcgttcgg ttcgacggcg
    14941 cgaggaagcc cgttcctcag gcgctgcgct ccttgcgcac acgcggctcc tgccgaggca
    15001 cgagcgtggg gttcaccccg gaacggacga cgtcgtggtc gatcacgcac ttcgcgacgc
    15061 cctcggtgga cggcagctcg tacatgacgt tcagcaggac ctcttccatg atcgcccgca
    15121 ggccgcgcgc cccggtgccg cggaggatcg cctggtccgc gatggcctgg agggcgtcgt
    15181 ccgcgaactc gagatccacg ccgtcgaact cgaagaagcg gcggtactgc ttcaccaggg
    15241 cgttgcgcgg cttggtgagg atgtccacga gggcctgctt gtcgaggttg tggacgttcg
    15301 tgagcacggg gagccgtccc acgaactcgg ggatcaaccc gaacttcaac aggtcttcgg
    15361 gcaggacccg gccgaggatc acgccgaggt ccttctcgtc cacgcgccgg acgtccgcgc
    15421 cgaagcccac gccgcggcta cccacacggc tctcgatgat cttctccagg cccgcgaacg
    15481 caccaccgca gatgaacagg atgttcgtgg tgtcgatctg gatgaactcc tggtgcgggt
    15541 gcttgcgtcc gccctgaggc ggaaccgacg cggtcgtgcc ttccaggatc ttgagcagcg
    15601 cttgctgcac gccctccccc gagacgtccc gggtgatcga tgggttctcg gacttgcggg
    15661 cgatcttgtc gacctcgtcg atgtagatga tcccggtctc ggccttcttc acgtcgtagt
    15721 cggcggcctg gatcagcttc aacaggatgt tctccacgtc ctcgcccacg tagccggcct
    15781 cggtgagggc ggtcgcgtcc gcgatggcga agggcacgtt cagcatcttg gcaagggtct
    15841 gagccagcag cgtcttgccg cacccggtgg ggccgaggat caggatgttg gacttctgga
    15901 gctcgacctc gccgtccttg gccccgacct ggatccgctt gtagtggttg tacacggcca
    15961 ccgacaggac cttcttggcc tgttcctggc ctatcacgta gtcgtcgagg aaggcgtaga
    16021 tctcctgggg cttggggagc tcatccagct tcagctcgga ggtctcggag agctcctcct
    16081 cgatgatctc gttgcacagg tcgatgcact cgtcgcagat gtagacgccg ggaccggcga
    16141 tgagcttctt cacctgcttc tgactcttcc cgcagaacga gcacttcagc aggtccgagt
    16201 caccgagtcg acccatcgtg cccccgagcc ccctatccga accgggtcgg ccgagcggca
    16261 cccggccaac gttcgtctgc ctgcgtgatt ctatgaccct ccggcggccg ccgctgcgag
    16321 ttccggatgc gccttccggc tggagatgac ctcatccacc acaccgtact ccaccgcttc
    16381 ctcggccgtc aggatgtaat cccgatccgt gtccttggcc accttgtcca gagactgccc
    16441 ggtgtgcttc gcgatcagct cctccaggag cttgcggtac cgggtgatct ccttggcctg
    16501 gatctcgatg tcgacggcct gtccctgggc cccgccgtgc ggctggtgca gcagcacgcg
    16561 agagtgcgga agcgcgtagc gcttgccctt ggtgccggcc agcagcagca ccgcggccgc
    16621 ggacgcggcc tgccccatca cgatggtgga cacgtccggc ttgatgtact gcatcgtgtc
    16681 gtagatcgcg aacagcgccg tgatgtcgcc acccggcgag ttgatgtaca ggttgatgtc
    16741 cttctcggga tcctcgctct ccaggtgcag cagctgcgcc atgatgaggt tcccgacgtt
    16801 gtcgtcgatg ggggtcccca ggaagacgat gcgctccttc agcaggcgcg agtagatgtc
    16861 gaacgagcgc tccccgcggc tggtctgctc cacgacgacg ggaacgaggt agttcgtgag
    16921 ttcgctcatg tcccttcctc cgctggttcc tgctagggag gctcggatgc ttcctcggcc
    16981 tcggcctcgg ccggcgtcga tgcgcggccc tcgccttcgg atgtgatctc ggcctgctcg
    17041 acgagaaggt cgagtgcctt cgaccggatg atatcactgg ccagtgctac gacctgaccg
    17101 ctcgcttcca gggtcttggc cacctccttg ggctcccgcc ccagggccgt tgcgagggaa
    17161 gcgacctcgc gcccgagctc ctcggcggtg acctccagct cctcgttccg ggccacggac
    17221 tcgagcacca ggtcggcctt gatggctcgc agcgcgtggg cccgtgcatc ggagcggaac
    17281 cgcagctcgt cgaacccctg cgcctcgagc agctgctcca gcctcatccc ggcgcgctcg
    17341 gctcgctcgc gcgcgtgctg cacccggtgc tcggtctcct cgtcgaccag ggtgtccggc
    17401 aggtccaggt ccaacgaatc caccagcttc tgcaagacca ggtcacggac ctccgcgtcg
    17461 gcgttccgct ccttgttcac cgcgatctgc tcccgcaggc tctccttgag ctcgtcgagg
    17521 gtgtcgaact cggaggccgt cttggcgaac gcgtcgtcgg ccgcagggag cttcttgccc
    17581 ttcacctcct tgaccaggac ctggaacgag acctcctggc ccgagcgctc gccgaaccgc
    17641 tcgtcgagca cggcgttgaa gcggaggatc tcccccgccc gcttgccctc cagctcctcg
    17701 tcgagcttgc cggtgaaccc ctcggacccc acctcgtaca ggtagtccgg ccgggtggcc
    17761 tcgggcacct cctggtcgtg gatggtggcc cgcaggtcga tcacgacgta gtcgcccttc
    17821 cgtgccggat gcgcggccgt ctccagctcc gcgaaccgct cccggagccc gtcgatcatc
    17881 tcgtcgacct ctgactcggc cacctcggtg gatggacgcc ggacctcgag ccccttgtag
    17941 tcgcgctcct cgagcttcag ccggggccgc acctccatgg ttgcggtgaa gatcaggggc
    18001 ttgccctcct ccacctgctc cagggagatg tcgggctcgg agatgggcgc caggtcgttg
    18061 tcgcggaccg cctcgcggta gtagctcggg accgtctcct ccaggaactc gctgagcacg
    18121 gtgtcgcgcc cgatctgggt gtcgatgatc cgccggggca ccttcccctt gcggaaaccg
    18181 ggcaccctga cctgctgcgc gacccgccgg tacgtgcggt ccaggtcctt gcccaggcgt
    18241 tcggcgggga tctcgaccgt gagcttgacg atgtgcttgt cggtctcttc gacggtggtc
    18301 tgcatcggga ctcgaagggt aacggaaatc ggtgcgcctg ctgcctccgg cggcccagcc
    18361 accgggggct cgggtaccgt ggagcggtgc tggaggagga gcttgccttc gcgcgccagg
    18421 tggccgacga ggcggaccgg atcgccctgt ccttcttcgg ctcggacccg gcggtgcgcc
    18481 tgaagcccga ccggaccccg gtgaccgagg ccgacgagcg gatcgaggcg atggtgcgcg
    18541 cacgtctggc gcagcggttc ccgcaggacg cgatcctggg tgaggaagag ggatctctgg
    18601 gtaccggcga ccggcgctgg gtcc
//
LOCUS       DASQQQ010000012         3715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_386509, whole genome shotgun sequence.
ACCESSION   DASQQQ010000012 DASQQQ010000000
VERSION     DASQQQ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386509"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VE646_00530"
     CDS             <1..216
                     /locus_tag="VE646_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78515.1"
                     /translation="RADLAKIIDMFESHTSDSGSHTGRWVLFLMAAGGAAYYAWSKVA
                     GGDGSMLGQQASDTVTGVRSGTSGAAA"
     gene            259..1146
                     /locus_tag="VE646_00535"
     CDS             259..1146
                     /locus_tag="VE646_00535"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HZD78516.1"
                     /translation="MALYRLVDPEPDRPLRSPALVAAFDGWVDAAGASTAAASHIAQG
                     ARQLAAFDPDALYDYRARRPVLDIVDGVPTKLAWSELSMRWSAEEHRDLLILSGPEPD
                     FRWRELAGDVLELCRRFGVTRWVSLGSIPAALPHTRPVPVMATASRPGLLQHGEQQGP
                     PGLLRVPSAMLSTLEIAVSAGGLPAVGYFAQVPHYVSGPYAAGSIALLDHLSRHLEVE
                     IPLGQLPDEAMAQRERLDDAIGQDSDSKSYIERLEALADEERLPTGDDLAAEVERFLR
                     DAGRGESGPGSGPTGPLPS"
     gene            1241..1777
                     /locus_tag="VE646_00540"
     CDS             1241..1777
                     /locus_tag="VE646_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78517.1"
                     /translation="MRWRDEYLGYLAVIVGFGAVVLWAEEVNPSVVAALAVAAALLFL
                     VMLANIARTGEVLLVGDRRDAPVQLMEEILDEAGYEVRRCQGPDVRSCPVDVGDPCPV
                     HCRALAAVVVGWSGGAGVRAPCGRALHVPALVVEEDTAAEPQITTVIDEGWERRGYVG
                     WERGPEAVVGTLDRILTA"
     gene            1830..2498
                     /locus_tag="VE646_00545"
     CDS             1830..2498
                     /locus_tag="VE646_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015114232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZD78518.1"
                     /translation="MPTDTTPPSERTRVRRHPDRAAYDRATIDAILDEALFCHVGYVD
                     GQGRPRVIPTIHGRVGDTLYIHGSNASRTLRALRDGVDVCVVATLLDGLVLARSAFNH
                     SMNYRSVIVYGVAREVTDPNEKWMAQKALVDHVCSGRSDQARMPNQRELDQTTLLAIG
                     MAEASAKVRTGPPKDDEDDFDLPIWAGVLPLRLEPRGPEPDPALATDLDAPPNVTGYR
                     RPGR"
     gene            2528..>3715
                     /locus_tag="VE646_00550"
     CDS             2528..>3715
                     /locus_tag="VE646_00550"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD78519.1"
                     /translation="MNQPSLRQNVRALPRAAWVLFAGTFVNRFGTFIIPFLTLYITAR
                     GYSAPQAGLGVAAYGLGAVGAQGFGGVLADRLGRRNTIALSMFTGAVLAFLLPEVRGL
                     PVILLVVMLLGMFGEIYRPASAALIADLVPMERRVTAFTFMRLAVNAGFAFGLAAGGA
                     LADRSFTLLFWADAATSAVFGAIALTALPEGVRTSRHEERRHASSAIRAITADRGFLL
                     FLAGVFAIAVIYMQNATTFALQVRRLGFSNTTYGLLLSLNGGLILFLELLVTSWTQRR
                     DRIRMVALGAALIGLGFGTLVVAHSILALVGMTLIFTMGEMIESPNASAFVADRSPRH
                     TRGRYQAAFGTMFAVAAIVGPIAGTSAFHADPNAVWMGCAVLGLIAAVLALSVNRRPA
                     PDLE"
BASE COUNT          479 a         1343 c         1316 g          577 t
ORIGIN      
        1 cgggcggatc tggccaagat catcgacatg ttcgagagcc acacctcgga cagcggctcg
       61 cacaccggac gctgggtcct gttcctgatg gcggccgggg gcgcggccta ttacgcctgg
      121 agcaaggtcg ccggcggaga cggctcgatg ctgggtcagc aggcgtccga caccgtgacc
      181 ggggtccggt ccggcacatc cggcgccgcg gcgtagcgtc ccctcggagg tccgccggcc
      241 gcgactatgc tgccgggcat ggccctgtat cggctcgtgg atccggagcc ggaccggccc
      301 ctgcggtcgc cggcgctcgt ggccgcgttc gacggctggg tggacgcggc cggcgcctcc
      361 accgccgccg cttcacacat cgcgcagggt gcgcgccagc tggccgcgtt cgatccggac
      421 gcgctctacg actaccgggc tcggcgcccg gtgctcgaca tcgtcgacgg cgtgcccacg
      481 aagctcgcat ggtccgagct gtcgatgcgc tggtcggccg aggagcaccg ggacctcctg
      541 atcctgtccg gccccgaacc cgacttccgg tggcgcgagc tggccggaga cgtgctggag
      601 ctctgccgac ggttcggggt gacccgatgg gtgagcctgg gatccatccc ggccgccctg
      661 ccccacacgc gaccggtgcc cgtcatggcc accgcctcgc ggccgggcct cctgcaacac
      721 ggcgagcagc agggtccacc gggactgctc cgggtgccct cggccatgct ctcgaccctg
      781 gagatcgcgg tgtcggcggg cggcctcccg gcggtggggt acttcgctca ggtcccccac
      841 tacgtgtccg gtccctacgc cgccggctcg atcgcgctcc tcgaccacct gagccgacac
      901 ctggaggtgg agatccccct gggccagctg ccggacgagg ccatggccca gcgggagcgc
      961 ttggacgacg ccatcggcca ggactcggac tcgaagagct acatcgagcg gctggaggcg
     1021 ctcgccgacg aggagcgcct gcccaccggg gacgatctgg ccgccgaggt cgagcgattc
     1081 ctgcgtgatg ccggacgcgg cgagagcggt ccagggtcgg gtcccaccgg cccgctgccc
     1141 agctgagccg cccgacgacg ttcggcccca tccgtcgggg acgttgggcc ctgaccggcc
     1201 gccgtccgtc gagccaccgt gagcccgagg agggacgacg atgcgctggc gcgacgagta
     1261 cctcggatac ctggccgtga tcgtggggtt cggagcggtg gtgctctggg ccgaggaggt
     1321 gaaccccagc gtggtggccg ccctggcggt ggcggccgcg ctgctgttcc tggtgatgct
     1381 ggcgaacatc gcccgcaccg gcgaagtgct gctggtgggc gaccgacggg acgccccggt
     1441 ccagctgatg gaggagatcc tggacgaggc cggctacgag gtccggcgct gccagggtcc
     1501 cgacgtgcgt tcctgcccgg tcgacgtggg tgacccctgc cccgtgcact gccgggccct
     1561 ggccgcggtg gtggtcggct ggtctggcgg cgccggggtt cgggccccgt gcggacgagc
     1621 gctccatgtc cccgccctgg tggtggagga ggacaccgcg gccgagccgc agatcaccac
     1681 ggtgatcgac gagggatggg agcgccgcgg ctacgtaggc tgggagcggg gacccgaggc
     1741 ggtcgtgggg acgctcgacc gcatcctgac ggcgtaagct cgcacacgcg ccgcgacggt
     1801 cgcgccgggc cttccactac catgcccgca tgccgaccga caccacccca cccagcgaac
     1861 gtacgcgggt ccgccggcac cccgatcggg cggcctacga tcgggcgacg atcgacgcca
     1921 tcctggatga ggcgctgttc tgccacgtgg ggtacgtgga cgggcagggc cggcccaggg
     1981 tgatccccac gatccacggg cgggtcgggg ataccctgta catccacggt tcgaacgcca
     2041 gccgcaccct ccgcgccctc agggacggcg tcgacgtctg cgtggtcgcc accctcctgg
     2101 acgggctcgt gctggcccgg tcggcgttca accactcgat gaactaccgg tcggtgatcg
     2161 tctacggggt ggctcgagag gtcaccgacc ccaacgagaa gtggatggcc cagaaagccc
     2221 tggtcgatca cgtctgctcg gggcgctccg atcaggcccg gatgccgaac cagcgcgagc
     2281 tcgaccagac cacgctcctc gcgatcggca tggccgaggc ctccgccaag gtgcggacag
     2341 gcccgccgaa ggacgacgag gacgacttcg acctgccgat ctgggcgggc gtgctgcccc
     2401 tgcgcctcga gccccgaggg ccggagccgg accctgccct ggcgaccgac ctggatgctc
     2461 ctcccaacgt gaccggctac cgacggccgg gccgctgatg cagctcgacc cgtaagctgg
     2521 tccccctgtg aaccaaccct cgctccgcca gaacgtccga gcgctgcctc gtgccgcgtg
     2581 ggtcctgttc gccggcacgt tcgtcaaccg cttcggcacc ttcatcatcc cgttcctgac
     2641 cctgtacatc acggcccgcg ggtattcggc cccccaggcc ggtctggggg tggcggccta
     2701 cgggctcggc gcggtgggcg cccaaggctt cggcggcgtg ctggccgacc gtctgggccg
     2761 acggaacacg atcgctctct ccatgttcac tggcgcggtg ctggccttcc tcctgcccga
     2821 ggtccgcggt ctgcccgtga tcctgctcgt ggtcatgctg ctcgggatgt tcggcgagat
     2881 ctaccggccg gcgtccgcgg ccctcatcgc cgacctggtg cccatggagc ggcgggtgac
     2941 cgcgttcacc ttcatgcgcc tcgccgtgaa cgcgggcttc gccttcgggt tggcggcggg
     3001 aggagcgctg gccgaccgat cgttcaccct cctgttctgg gcggatgccg caacctcggc
     3061 cgtcttcggc gcgatcgcgc tgaccgcgct gcccgagggc gtccgcacgt ctcgccacga
     3121 ggaacgccgc cacgcatcga gcgcgatacg cgcgatcacg gccgaccgag ggttcctgct
     3181 gttcctggcg ggcgtgttcg cgatcgccgt gatctacatg cagaacgcca ccaccttcgc
     3241 cttgcaggtc cgccgtctgg gcttctcgaa cacgacgtac gggttgctcc tgtccctgaa
     3301 cggcggcctg atcctgttcc tggagctgct ggtgacgtcc tggacccagc gccgcgatcg
     3361 gatccggatg gtggcgctgg gcgccgcact gatcgggcta gggttcggca cgctcgtggt
     3421 cgcccactcg atcctcgcgc tcgtgggcat gacgctgatc ttcacgatgg gcgagatgat
     3481 cgagtcgccg aacgcctctg ccttcgtcgc cgaccgctcc ccgcggcaca cgcggggccg
     3541 gtaccaggcg gcgttcggga ccatgttcgc ggtggccgcc atcgtgggac ccatcgccgg
     3601 cacgagcgcg ttccacgccg atccgaacgc cgtgtggatg ggctgcgcgg tgctcggcct
     3661 gatcgccgcc gtcctggccc tgtcggtgaa ccgccgtccg gctcccgacc tggag
//
LOCUS       DASQQQ010000013        11124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_28259, whole genome shotgun sequence.
ACCESSION   DASQQQ010000013 DASQQQ010000000
VERSION     DASQQQ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11124
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28259"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /gene="sbcD"
                     /locus_tag="VE646_00555"
     CDS             complement(<1..599)
                     /gene="sbcD"
                     /locus_tag="VE646_00555"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HZD78520.1"
                     /translation="MRFLHTGDWHVGRAIRGRSRSDEFAEVLDEVVAIAVDEAVDCML
                     LAGDIYEHRSASPEADAIVFEALIRLHEAGIRVVAVPGNHDSALRLEALGKLLRPIGV
                     HVAARVAPPDAGGIVEVPSRDGATVAEVACVPFVPERRFGDAAELFRAGESWYTSYAE
                     GMGNLLAAMASGFTPGRISILLAHLFTDGAMVTPGGGERA"
     gene            complement(602..2287)
                     /locus_tag="VE646_00560"
     CDS             complement(602..2287)
                     /locus_tag="VE646_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZD78521.1"
                     /translation="MDEVSEQVGMVLGTEDATPLTFWVGVAPDAYLQLDDAVVVDTQV
                     PGRGALRIAGIVQDVRARHEGAQFDTDVFLVDRGVLPVDTAVAAQVVATRFEPEIFVP
                     PMPGLPAYRARGKDRDEALYFDQMERRVGAGLTRDGEPVFLDLDFLDGSRGAHVNISG
                     ISGVATKTTYALFLLYALFHSDVLGRYAANTKGIVFNVKGEDLMWLDTPNVRLTDGDR
                     EDYQRLGLPAGPFESVGLWAPVRPDAGDKAIPHVEGRSEGVAAYYWTIREFVREKFLR
                     FMFAEAEDERSQIADLVARVEAALDRECDDDPEHDATVTFGGYPVHDLPQLCQIITQE
                     VDADGSSWRGRVSDATVAAFIRRLDAARFRMGHLIWGRDAEDASAHRIDWEANQVTVI
                     DIHSLHDRAKRFVTGVMIKRLFEHKERMGRREPLAFLVLDELNRYAPREGWSPIKEVL
                     LDVAERGRSLGMILVGAEQTASEVERRIIANSAFRVVGRLDSAEAQRSEYGFLPAVTR
                     SRASILKPGSMILQQPHIPVPLQIRFPFPSWATRADEAAPTDAAAVFRRFEGE"
     gene            complement(2280..3242)
                     /locus_tag="VE646_00565"
     CDS             complement(2280..3242)
                     /locus_tag="VE646_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78522.1"
                     /translation="MLALRADPWTPDHGMGFEAAPDEPPARAEPFVETEDWCAPRRPR
                     PREQGPVWFVDGVRRVELRLLAEDGGRRAFGLFGTYAVGAVRSDGPAVFADHQVDRAV
                     VVGGGLQPDEVRVAVGGMSLAYRPVSDSGSEADEPLHRLQREMRRAESTLAIGVAATE
                     GELVLADGRWTSQQPTESPVVGVVKRWSREYLEPEQEHLVARLGPGERTPLFGLADPD
                     QPVERYAWYARLVPLRPAWHDHAGVVRCEVRAGIGLDAAVALADRVSALLPAFSGRPS
                     DPRYPQNLAPVAALESWLQHRMGHRGLIRRALMAVVTGWGTGGG"
     gene            complement(3774..4940)
                     /locus_tag="VE646_00570"
     CDS             complement(3774..4940)
                     /locus_tag="VE646_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014687948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZD78523.1"
                     /translation="MTDLERYELPEELEEFRQLVRRIAEDKVAPRAAEIDVSDEWPED
                     VYRVLVDNELMGVGYPEEHGGSGGGALAFGVFIEELSRVSAGVSLAPLVAKLGVIPML
                     VAGDEDKAGRMIRGIAGGELLMSYALTEPEAGSDPGSMTTRYRREGDTFILDGTKRFI
                     TGAGVSHAYVVFATSDPSLRSKGVSAFLVMRDDPGVSFGRKEAKMGIRGSPTAEVIMD
                     GCRIPADRMIGEEGRGFAYAMKTLDHSRPAIAAQALGIAQGAFEIASHHAAERHQFGK
                     AIAEFEGVQFMLADMAMRLEASRMLVYKALTLCDADDPRMPYFSSIAKCFASDAAMAV
                     TTDAVQVLGGYGYMRDYTAERFMRDAKITQIYEGTNQIQRVVIARQIMKTLGAA"
     gene            complement(4958..6145)
                     /locus_tag="VE646_00575"
     CDS             complement(4958..6145)
                     /locus_tag="VE646_00575"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZD78524.1"
                     /translation="MPTSVIVAGARTPIGKHRKALAALSGVELGTVAIREALSRAGVP
                     PQRVEYVIMGQVLRAGTGQITARQAAVAAGVPKEIPALNIDKVCLSGIDAIALADALI
                     RAGELDLVVAGGMESMSQAPYLAPAAREGQRLGHTSFVDSMVRDGLWSTFLKQVMGES
                     SDQLNDDLGITREEQDAWAARSHRRAHEAWESGAMSEEVVPVEVPQRKGPPLVVDRDE
                     GVRPDTTAQVLAGLKPAFREGGTITAGNAAQISDGAAAVVVASRERAEELGLAPMVEV
                     VAHGMAAERYAYLHTVPAIALQNALKKAGLDVHDLGLVEVNEAFAAVSLNVTKILGVS
                     EDIVNVNGGAVSLGHPIGMSGARIVLTLAHEMRRRGVDLGGATICGGGGQGDALVLRR
                     VAD"
     gene            complement(6235..8052)
                     /locus_tag="VE646_00580"
     CDS             complement(6235..8052)
                     /locus_tag="VE646_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZD78525.1"
                     /translation="MRKTERSTATLLQGSEIMRAGKADVFKVRERTRRRRVWRLFLIL
                     GAIDLFLWYRYATHNPLRPPAFGPQWVIWFPILFLFLIILLMMAMPLFSGRSPHLLVR
                     PEDIEVGLTEVQGLDTQVDEVIRTLDVFLGYATFRGELGGNPRRGILFEGPPGTGKTY
                     LAKAMAKQAGVPFLFISAPAFQSMWFGMTGARIRSFFRQLRKLARKEGGAIGFIEEID
                     AIGNDRGGGLNGASPDGTGVGRTVSNMFGPGGSGMVNELLIQMQSFDTPTARRRLSAR
                     FVEWVNGYLPPEHRWRVGKPEYNNILLIAATNRADSLDAALLRPGRFDRRLYFDLPTK
                     QGRRDLIDFFMERKAHHQQLDEDDVRERLAHDTFGYTPVMIEHLFDESLLVALRGGRR
                     AMNLDDVYEAKLTDEIGIKQPVVYTEDERASVATHEAGHAAVAYFFGKGRRLEVLSII
                     KRRQALGLLAHGDLEERFTRSRSDIEAHIAIALGGLVSEELFIGESGTGPASDLAGAT
                     QLAVQMVGSLGMAGSLISYEAVAEGPLSKNLVGKVLADKDAKQRVEDILAAQKERVTA
                     LLDANRDVVMALRDALMERDELVGEGIITVIEKALAERS"
     gene            8207..8581
                     /locus_tag="VE646_00585"
     CDS             8207..8581
                     /locus_tag="VE646_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78526.1"
                     /translation="MARKKQTPITEEDARTVGALLRGLRRSAGFRAVQDAAGAQGCPA
                     ARQTIYAYERGALTPSLKQFLELVEFYALGDHATEPAKPSEDLRVQAVAAIHRALRLP
                     AYQVVTAIDLIARLQPPLPITR"
     gene            8589..8990
                     /gene="mce"
                     /locus_tag="VE646_00590"
     CDS             8589..8990
                     /gene="mce"
                     /locus_tag="VE646_00590"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017538324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HZD78527.1"
                     /translation="MLTDIDHVGIAVEDLDAAVAQHVRLFGVEPVHRERVEAQGVEEV
                     LFAVGTSFIQLLGALGPDTPVGRFLASRGPGVHHVAYRVDDVAAALDRLRAEGVRLID
                     EEPRPGSRGTTIAFAHPSGMGGVLVELVQES"
     gene            8987..>11124
                     /locus_tag="VE646_00595"
     CDS             8987..>11124
                     /locus_tag="VE646_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZD78528.1"
                     /translation="MITCPACSADNPDGARFCNACGAALAVPEQVRKERKFATALFAD
                     LVGSTALAEREDPEVVQAVVGQTFDRLAAEIGRYEGLLEKFMGDAVLAIFGVPRAHED
                     DAERAVRASIEMLAVLSELNRGFEVEGKPTLAMRIGIEAGEVLVDLERATGAARDRML
                     TGDAVNTAARLQAAAEPGAVVVGPTVYASTKDVIEYRALEPLMLKGKAEPVPAWRALR
                     IKARTRGERPRLGLEARLVGRDEELSVLKQTLRRVESEGRPALVTVVGPAGVGKSRLV
                     AELERYAEGLPQTVYWRRGRCLAYGNISYSAFADAIKAQCEIFEDDPADVAAGKADAA
                     VRELFGDGSLARQIRALVGAGEARDMSREDLFDAWRRFLERLAARYPLVLVLDDLHWA
                     DEGLLDLVDHVADWAQGPIMVVATARPELFEMRPTWGGGKRNAASIYLDPLSEAEGAT
                     MLRDLLPGPVDEQLSRAIVERSEGNPLYVEEIVRKLIDDGVLRATEASRWEVAQRVED
                     VQLPRSIQGLIAARLDGLPDHEKAVLQDAAVVGRVFWVGAVAELTGRPLAEVRDALGR
                     LRVKELVVPHDPSSFSDQLEFSFRHNLIRDGAYESLPKTMRADKHAGVARWAEQRAGD
                     RADEIAELIATHLLEALRYLDELGETSRRRDDLGSSAFRWAAAAGERASALWQRAPAT
                     RWFREAERLADDLELPPRERVRLVRAHIEAS"
BASE COUNT         1617 a         4095 c         3840 g         1572 t
ORIGIN      
        1 gctcgctccc ccccgccggg ggtaaccatg gcgccgtcgg tgaacaggtg cgccagcagg
       61 atcgagatcc gccccggggt gaagccggag gccatcgccg cgagcaggtt gcccatgccc
      121 tcggcgtacg aggtgtacca ggactcgccg gctcggaaca gctcggccgc atcgccgaac
      181 cgccgctccg ggacgaacgg gacgcaggcc acctcggcca cggtggcgcc gtcgcgcgag
      241 ggcacctcga cgatcccgcc ggcatcgggg ggggccaccc gcgccgccac gtgcacaccg
      301 atcgggcgca gcagcttgcc gagcgcctcc agccgtaacg ccgagtcgtg gttgccgggg
      361 acggcgacca cccggatccc ggcctcgtgc agccggatca acgcctcgaa gacgatggcg
      421 tccgcctccg gcgaggccga gcggtgctcg tagatgtccc cggccagcag catgcagtcc
      481 accgcctcat cgacggcgat cgccaccacc tcgtcgagga cctcggcgaa ctcgtccgat
      541 cgcgaccgcc cccggatggc ccggcccacg tgccagtcgc ccgtgtggag gaaccgcacc
      601 ctcactcccc ctcgaaccgc cggaacaccg cggcggcatc ggtcggcgcc gcctcgtcgg
      661 cccgggtggc ccaggagggg aaggggaacc ggatctgcag gggcaccggg atatgcggct
      721 gctgcaggat catcgagccg ggcttcagga tcgaggcgcg tgagcgcgtc acggccggca
      781 ggaacccgta ctcgctgcgc tgagcctcgg ccgagtccag gcgtcccacc acccggaagg
      841 ccgagttcgc gatgatccgc cgctccacct cgctggcggt ctgctcggcg cccaccagga
      901 tcatgcccag cgagcgcccg cgctcggcca cgtcgagcag gacctccttg atggggctcc
      961 aaccctcgcg cggcgcgtag cggttcagct cgtccaggac caggaaggcc agcggctctc
     1021 gacgacccat ccgctccttg tgctcgaaca ggcgcttgat catcacgccg gtcacgaagc
     1081 gcttggcccg gtcgtggagg ctgtggatgt cgatcacggt gacctggttc gcctcccagt
     1141 cgatccggtg ggccgaggca tcctcggcat cccggcccca gatcaagtgc cccatccgga
     1201 atcgagccgc gtccaagcgg cggatgaacg ccgctaccgt ggcgtcggag acgcgccccc
     1261 gccacgacga gccgtccgcg tccacctcct gcgtgatgat ctggcagagc tgggggagat
     1321 cgtgcaccgg gtagccgccg aaggtgacgg tggcgtcgtg ctcgggatcg tcatcgcact
     1381 cccggtccag cgcggcctcc acccgggcca ccaggtccgc gatctgggag cgctcgtcct
     1441 cggcctcggc gaacatgaac cgcaggaact tctcccggac gaactcgcgg atcgtccagt
     1501 agtaggcggc cacgccctcc gagcgacctt ccacgtgcgg gatcgccttg tctccggcgt
     1561 cggggcgcac cggcgcccac agcccgacgc tctcgaacgg cccggccggc agccccagcc
     1621 gctggtagtc ctcacggtcg ccgtcggtca ggcgcacgtt cggggtgtcg agccacatca
     1681 ggtcctcgcc cttcacgttg aacacgatgc ccttggtgtt cgcggcgtac cgccccagca
     1741 cgtcagagtg gaacagcgca tagagcagga acagcgcgta cgtggtcttc gtggccaccc
     1801 cggagatgcc ggagatgttc acgtgcgcgc cccgcgaccc gtccaggaag tccaggtcca
     1861 ggaacaccgg ctcgccgtcc cgggtcagcc cggcccccac ccggcgctcc atctggtcga
     1921 agtacagggc ctcgtcgcgg tccttgcccc gggcccggta ggcgggcagc ccgggcatcg
     1981 gcgggacgaa gatctcgggc tcgaaccgcg tggccaccac ctgcgccgcg accgccgtgt
     2041 ccaccgggag caccccccgg tccaccagga acacgtcggt gtcgaactgc gccccctcgt
     2101 ggcgggcccg cacgtcctgc acgatcccgg cgatccgcag cgctccccgg cccggcacct
     2161 gcgtgtccac cacgacagcg tcgtccagtt ggaggtaggc atcgggggcc acacccaccc
     2221 agaaggtgag cggggtggcg tcctctgtcc ccagcaccat cccgacctgt tcgctgacct
     2281 catccaccgc cggtccccca tcctgtcacc acggccatca acgctcgccg gatcagcccc
     2341 cggtgaccca tccggtgctg gagccaggac tcgagcgcgg cgaccggggc gaggttctgt
     2401 gggtagcgcg ggtcgctcgg ccggccggag aacgccggca gcagcgcgga cacccgatcg
     2461 gccagcgcga ccgcggcgtc caggccgatc ccggcccgca cctcgcagcg cacgacgccc
     2521 gcgtggtcgt gccaggctgg gcgcagcggc acgagccgcg cgtaccacgc gtagcgctcg
     2581 accggctggt cggggtcggc cagcccgaac aagggcgtgc gctctccggg cccgagccgg
     2641 gccacgaggt gctcctgttc cggctcgagg tactcgcggg accagcgctt gaccacgccc
     2701 accaccgggc tctcggtggg ctgctgcgac gtccaccgcc cgtcggcgag caccagctcc
     2761 ccctcggtgg ccgcgacgcc gatggccagc gtgctctccg ctcggcgcat ctccctctgg
     2821 aggcggtgca ggggctcgtc ggcctccgag cccgaatcgc tcaccgggcg atacgcgagc
     2881 gacatgccgc cgaccgccac ccggacctcg tcgggctgga gcccgccccc caccacgaca
     2941 gcccggtcca cctggtggtc ggcgaagacc gccggtccgt ccgagcggac cgcgccgacc
     3001 gcataggtac cgaagagccc gaaggctcgg cgaccgccgt cctcggccag cagccggagc
     3061 tcgacccgtc ggacgccgtc gacgaaccac acgggtcctt gctccctcgg acgtggtcgg
     3121 cggggggcac accagtcctc cgtctccacg aacggctccg cccgagcggg cggttcgtcc
     3181 ggcgccgctt cgaaccccat gccgtgatcc ggcgtccagg gatcggcccg cagggcaagc
     3241 atggctcgaa gcttagcgac cgcgtcggac gggcccaagc acgccttcag ccatcggcac
     3301 aggcagcgcc tcggccccct atcccccatc cgggggactt caccagaccg cccacgcctg
     3361 ccgatacagg ggagaagtga cgaccttctc cgggacggac gcggcagcgg catgggatgg
     3421 ggcacctcgg gctgcgggtg cggtgaccga cccgcctctt cgcctcgtcg gcatccctcc
     3481 cggatcggaa caggccgcac gatcgggcga accaccccgt caacgaagct cgcttcggcg
     3541 tcgcagcacc gaccgatgac ggccgtatgg cgctgagggc gaacgaacgg acggagcggg
     3601 agccggcgcc ggacacgacc ggcggcgggc cccccgcgcc gcaggctgga cccgcggctc
     3661 ccgctcctcc gacccgggcc cccaccacgc cgagcggtgg gctcgcgatc gatcccgagg
     3721 acccgaggct cctgctgcgc cacctggcct cccggggcgg ggaggagggg tagctaggcg
     3781 gcgccgagcg tcttcatgat ctggcgggcg atcacgacgc gttggatctg gttggtgccc
     3841 tcgtagatct gggtgatctt cgcgtcgcgc atgaaccgct cggccgtgta gtcacgcatg
     3901 tacccgtagc cgcccaggac ctggaccgcg tcggtggtca ccgccatcgc ggcgtccgag
     3961 gcgaagcact tcgcgatcga cgagaagtag ggcatccggg gatcgtccgc gtcgcacagg
     4021 gtgagcgcct tgtacaccag catccgcgag gcctccagac gcatcgccat gtcggcgagc
     4081 atgaactgca caccctcgaa ctcggcgatg gcctttccga actggtggcg ctcggccgcg
     4141 tggtgggagg cgatctcgaa ggcaccctgg gcgataccca gggcctgtgc cgcgatggcc
     4201 ggccgggagt ggtccagggt cttcatcgcg taggcgaacc cccggccctc ttcgccgatc
     4261 atccggtcgg ctgggatccg gcagccgtcc atgatcacct cggccgtggg cgagccccgg
     4321 atccccatct tggcctcctt gcggccgaac gagacgccgg ggtcgtcgcg catcaccagg
     4381 aacgccgaaa cgcccttcga gcgcagggac ggatcggacg tggcgaacac gacgtaggcg
     4441 tgggacaccc cggctccggt gatgaaccgc ttcgtcccgt ccaggatgaa cgtgtcccct
     4501 tcgcgacggt accgggtggt catcgagccg ggatccgacc ccgcctctgg ctcggtcagg
     4561 gcgtacgaca tcaggagctc gccaccggcg atccctcgga tcatgcggcc ggccttgtcc
     4621 tcgtcaccgg ccacgagcat ggggatcacg ccgagcttgg ccaccagggg ggccagggac
     4681 acgccggccg agaccctcga cagctcctcg atgaacacgc cgaaggccag ggcgcccccg
     4741 ccggacccgc cgtgctcctc cgggtagccc acgcccatga gctcgttgtc caccaggacc
     4801 cggtagacgt cctcgggcca ctcgtcgctc acgtcgatct ccgcagcccg cggtgcgacc
     4861 ttgtcctcgg cgatccgccg gaccagctgc cggaactcct ccagctcctc gggcagctcg
     4921 tagcgttcga gatcggtcat cccatcgtgg cccagactta gtctgccacc cgtctgagca
     4981 cgagcgcgtc tccctggccg ccgccgccgc agatcgtggc gccgcccagg tcgacgcccc
     5041 gtcggcgcat ctcgtgcgcg agcgtcagca cgatccgcgc gccgctcatc ccgatcggat
     5101 gaccgagcga gaccgctccc ccgttcacgt tcacgatgtc ctcggacacg ccgaggatct
     5161 tcgtcacgtt cagggagacc gcggcgaagg cctcgttgac ctcgaccagc cccaggtcgt
     5221 gcacgtccag accggccttc ttcaacgcgt tctgaagcgc gatggccggc acggtgtgga
     5281 ggtaggcgta ccgctccgcc gccatgccgt gcgccacgac ctccaccatc ggagccaggc
     5341 cgagctcctc ggcccgctcg cggctggcca ccaccaccgc ggcggcgccg tccgagatct
     5401 gcgccgcgtt gccggcggtg atcgtgcccc cttcgcggaa cgccggcttc aggccggcca
     5461 gcacctgggc ggtggtgtcc gggcggaccc cctcgtcacg gtccaccacg agcggcggcc
     5521 ccttgcgctg cgggacctcc accgggacca cctcctcgct catcgccccc gactcccatg
     5581 cctcgtgcgc gcgtcggtga ctccgggccg cccaggcatc ctgctcctcc cgggtgatgc
     5641 caagatcgtc gttcagctgg tccgacgact cccccatcac ctgcttcagg aaggtcgacc
     5701 acagcccgtc ccggaccatc gagtccacga acgacgtgtg cccgaggcgc tggccctcgc
     5761 gcgcggcggg ggccaggtac ggcgcctgcg acatcgactc catccccccg gccacgacca
     5821 ggtcgagctc gccggcccgg atcagcgcgt cggcgagggc gatcgcgtcg atcccggaca
     5881 ggcagacctt gtcgatgttc agggcgggga tctccttggg gacccccgcg gcgacggccg
     5941 cctggcgggc ggtgatctgc ccggtcccgg cccgcaggac ctgccccatg atcacgtact
     6001 ccacgcgctg gggcgggacg ccggcccggg acagggcctc ccggatcgcg acggtgccga
     6061 gctcgacccc ggacagggcg gcgagcgcct tccggtgctt gccgatgggg gtccgggctc
     6121 ccgcgacgat gacggatgtc ggcatacaga gactcctggt cctggtccgt ccagtctacg
     6181 atgccgcctg ttggcgccgg gacctggtcg ggatccgaga cccgggtcgg gccttcagct
     6241 ccgctcggcc agggccttct cgatgaccgt gatgatcccc tcgccgacca gctcgtctcg
     6301 ctccatcagc gcgtcgcgga gcgccatgac gacgtcacgg ttcgcgtcga gcagggcggt
     6361 gactcgctcc ttctgggcgg ccagaatgtc ctcgacccgc tgcttcgcgt ccttgtcggc
     6421 gagcaccttg cccaccaggt tcttcgacag cggcccctcc gccacggcct cgtacgagat
     6481 cagcgatccg gccatgccga gcgagcccac catctgcacg gcgagctggg tggccccggc
     6541 caggtcggag gccggccccg ttccggactc gccgatgaac agctcctcgg agaccaggcc
     6601 gccgagcgcg atcgcgatgt gggcctcgat gtcggagcgg gaccgggtga accgctcctc
     6661 caggtcaccg tgcgccagga ggccgagcgc ctgacggcgc ttgatgatcg acaggacctc
     6721 gagccgacga cccttgccga agaaatacgc gaccgcggcg tgccccgcct cgtgcgtggc
     6781 cacgctggcc cgctcgtcct ccgtgtacac caccggctgc ttgatcccaa tctcgtcggt
     6841 gagcttggcc tcgtagacgt cgtccaggtt catcgcgcgc cggccgcccc gcagcgccac
     6901 cagcagcgac tcgtcgaaga ggtgctcgat catcaccggc gtgtagccga acgtatcgtg
     6961 ggccagacgc tcccgcacgt cgtcctcgtc cagctgctgg tggtgcgcct tccgctccat
     7021 gaagaagtcg atcaggtcgc gccggccctg cttggtgggg aggtcgaagt acaggcgacg
     7081 gtcgaaccgg cccggacgca gcagggcagc gtcgagcgag tccgcgcggt tcgtggcggc
     7141 gatcagcagg atgttgttgt actcgggctt gcccacgcgc caccggtgct cgggcggcag
     7201 atagccgttc acccactcca cgaagcgcgc cgatagccgc ctgcgggcgg tgggcgtgtc
     7261 gaacgactgc atctggatca gcagctcgtt caccatcccg gagccacccg gcccgaacat
     7321 gttggacacg gtgcgtccca ccccggtgcc gtcgggggaa gcaccgttca ggccaccgcc
     7381 gcgatcgttg ccgatcgcgt cgatctcctc gatgaagccg atggccccac cctccttgcg
     7441 ggcgagcttg cggagctggc ggaagaacga gcggatccgg gcgccggtca tgccgaacca
     7501 catcgactgg aaggcgggcg ccgagatgaa caggaacggg acgccggcct gcttggccat
     7561 cgccttggcc aggtacgtct tgccggtgcc gggaggcccc tcgaacagga tcccccggcg
     7621 gggattgccc ccgagctcgc cccggaacgt cgcgtagccc aggaacacgt cgagggtcct
     7681 gatcacctcg tccacctggg tgtcgagccc ctggacctcg gtcaggccga cctcgatgtc
     7741 ctcggggcgg acgagcaggt ggggcgagcg gccgctgaac agcggcatcg ccatcatcag
     7801 caggatgatc aggaacagga acaggatggg gaaccagatc acccactgcg gcccaaacgc
     7861 cgggggtcgc agggggttgt gggtggcgta ccggtaccac aggaacaggt cgatcgcccc
     7921 caggatcagg aacaggcgcc agacccgtcg ccgacgtgtc cgctcgcgga ccttgaagac
     7981 gtcagccttc cccgcgcgca tgatctccga gccctgcaac agcgtcgcgg tcgaccgctc
     8041 ggtcttcctc acgcccaccc cctcatcccc tccaccgcct ttcatcgtag gcaaccctct
     8101 atcgggtcgc atccaacttt cggcctagag catctccgcg ccgttgcccg gtcgcgcgca
     8161 ggccggacga gccgatatcg tgcaaccctg gcctggatat gtacccatgg cgaggaagaa
     8221 gcagacaccc atcaccgagg aggacgcccg gaccgtgggg gcgctgctcc gggggctccg
     8281 ccgctcggcg ggcttccggg ccgtccagga cgcggccggg gcccagggct gcccggcggc
     8341 ccgacagacg atctacgcct acgagcgcgg cgccctcacc ccgtccctca agcagttctt
     8401 ggagctggtc gagttctacg cgctggggga ccacgcgacg gagccggcca agccctccga
     8461 ggaccttcgg gtccaggccg tcgcagcgat ccaccgggcg ctgcggcttc ccgcctacca
     8521 ggtggtcacg gcgatcgacc tgatcgcgcg gctccaaccg ccgctcccga tcacgcggta
     8581 ggaggcccgt gctcaccgac atcgaccacg tggggatcgc cgtcgaggac ctcgatgcgg
     8641 cggtcgcgca gcacgtgcgg ctgttcgggg tcgaacccgt gcatcgcgaa cgggtggagg
     8701 cccagggggt cgaggaggtc ctgttcgcgg tgggcacgtc cttcatccag ctcctcggcg
     8761 ccctggggcc ggacacgccg gtgggccggt tcctcgcctc ccggggcccc ggcgtgcacc
     8821 acgtggccta ccgggtggac gatgtggccg ccgcgctcga tcgcctgcgg gccgagggcg
     8881 tgcggctgat cgatgaggag ccgcgaccgg gatcccgggg caccacgatc gccttcgccc
     8941 acccatcggg catgggcggg gtcctggtgg agctggtgca ggagtcatga tcacgtgtcc
     9001 cgcatgctcg gcggacaacc cggatggggc ccggttctgc aacgcgtgcg gggccgccct
     9061 cgccgtgccc gagcaggtcc gcaaggagcg gaagttcgcc acggcgctgt tcgccgacct
     9121 ggtgggctcg accgccctgg ccgagcgcga agatcccgag gtggtacagg ccgtggtggg
     9181 tcagaccttc gatcgcctgg ccgcggagat cggccggtac gaggggctgc tcgagaagtt
     9241 catgggcgat gccgtcctcg ccatcttcgg cgtcccgagg gcgcacgagg acgacgccga
     9301 gcgggcggtg cgggcctcga tcgagatgct cgccgtgctc tccgagctga accgagggtt
     9361 cgaggtcgag ggcaagccca cgctcgcgat gcggatcggc atcgaggctg gcgaggtgct
     9421 cgtggacctg gagcgggcga ccggggccgc ccgagaccgg atgctcaccg gcgacgcggt
     9481 gaacacggcc gctcgactcc aggccgcggc ggagccgggc gccgtggtgg tcggaccaac
     9541 cgtctacgcg agcacgaagg acgtgatcga gtaccgggcg ctcgaaccgc tcatgttgaa
     9601 gggcaaggcc gagccggtcc ccgcctggcg ggcgctccgg atcaaggccc gcactcgcgg
     9661 cgagcgaccc cgcctggggc tcgaggcccg gctggtgggg cgggacgagg agctctcggt
     9721 gctgaagcag acgctccggc gggtggagag cgaggggcgg ccggcgctgg tcacggtggt
     9781 ggggccggcc ggcgtcggga agtcccgcct ggtcgccgag ctcgagcgct acgccgaggg
     9841 cctgccgcag acggtgtact ggcgacgagg gaggtgcctg gcgtacggga acatctcgta
     9901 ctcggcgttc gcggatgcca tcaaggccca gtgcgagatc ttcgaggacg atccggccga
     9961 cgtcgccgcg ggcaaggcgg acgccgccgt tcgggagctg ttcggagacg ggtccctggc
    10021 ccggcagatc cgagccctgg tgggggccgg cgaggcccgc gacatgagcc gcgaagacct
    10081 cttcgacgca tggcgccggt tcctggagcg gctcgcggcc cggtacccgc tggtcctggt
    10141 gctcgacgac ctccactggg cggacgaggg gctgctcgat ctcgtcgatc acgtggccga
    10201 ttgggcgcag ggcccgatca tggtcgtggc gaccgcccgc cccgagctgt tcgagatgcg
    10261 gccgacgtgg ggcggcggca agcggaacgc cgcctcgatc tacctggatc cgctgtccga
    10321 ggccgagggc gccaccatgc tccgggacct gctccccggt cccgtggacg agcagctcag
    10381 tcgcgcgatc gtcgagcgca gcgagggcaa cccgctctac gtcgaggaga tcgtccgcaa
    10441 gctgatcgac gacggcgtgc tccgggccac cgaggcctcg cgctgggagg tcgcccagcg
    10501 ggtcgaggac gtccagctgc cgcgctcgat ccaggggctg atcgccgcgc ggctggatgg
    10561 gttgcccgac cacgagaagg cggtcctgca ggacgccgcc gtcgtcggcc gggtcttctg
    10621 ggtcggcgcc gtcgccgagc tcaccggccg gccgctcgcc gaggtgcgcg atgcgctcgg
    10681 gcggctccgg gtgaaggagc tcgtggtccc ccatgatcct tcgtcgttct ccgaccagct
    10741 cgagttctcg ttccgccaca acctgatccg cgacggggca tacgagtcgc tgccgaagac
    10801 catgcgtgcc gacaagcacg ccggcgtcgc ccggtgggcc gagcagcgcg ccggcgatcg
    10861 agccgacgag atcgccgagc tgatcgccac ccacctgctg gaggcgctgc gttacctgga
    10921 cgagctcggt gagacgagcc ggcgccgcga tgacctcggg agcagcgcgt tccgatgggc
    10981 cgccgccgcc ggggagcgcg cctccgcgct ctggcagcgg gccccggcta ctcggtggtt
    11041 ccgggaggcc gagcggttgg cggacgacct cgagctgccc ccacgcgagc gggtgcggct
    11101 ggtccgggcc cacatcgagg cgtc
//
LOCUS       DASQQQ010000014         3720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_166118, whole genome shotgun sequence.
ACCESSION   DASQQQ010000014 DASQQQ010000000
VERSION     DASQQQ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166118"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VE646_00600"
     CDS             <1..729
                     /locus_tag="VE646_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZD78529.1"
                     /translation="VIWVNRTYVREVFGDVDPMGQRVYAGNEFRRVVGVVDDVPYDAE
                     GDTSRKLYIPHAQFADNRNWALIQTVKVRAGAASSREAIRKVLAGMDGQLVLYHPEMF
                     ADVVAGARAQDRFATVLMAAFALLALALSLVGTYGVLAGSVAGRTRELGIRMALGADA
                     RSVRRLVLRYATALIAPGVLIGLAGAWSGTRWLRALLFRVESGDPLVYAGVVALFAVV
                     GLVAGWAPARRATRVDPARALSME"
     gene            984..2186
                     /locus_tag="VE646_00605"
     CDS             984..2186
                     /locus_tag="VE646_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78530.1"
                     /translation="MAKDALDEDPMGASHSERYDHVVDRFTELEAAVAGGRVTRVRAA
                     GARDLEGRVLRTLRALGGEATPGDLVAILAHPRSDVEAALEALIASDRVQVKVLDPGD
                     VVYRLAGAGPHLAPRSGLSASPRRALSGPDRAPAAVRFDRKTLHLIRSLKGVISLAEL
                     VEHTGTSVAEANREMQRLVSWYGGEGHPSLDGHVVYAFPGLLTSVHTPPGTRAPRPAW
                     MRWGDPMGSRRLTESGSWVGRLAYSLGLLGFGRGLVRRVGAVRAIRFRQRSVLRRLAL
                     GHVFQAALAGSGVVSVGSARRYLALRTGYRIGRRRVEAVLRELSGEFDAPVTREGGEL
                     FFGFRNVKRQFLAAELVRRRLGLGRRVTGRTVFDSGDTPRAAAQREIEAFDRDLAFAH
                     SPRRRTPS"
     gene            complement(2330..2674)
                     /locus_tag="VE646_00610"
     CDS             complement(2330..2674)
                     /locus_tag="VE646_00610"
                     /inference="COORDINATES: protein motif:HMM:NF025475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HZD78531.1"
                     /translation="MNEHDAGSTPSGQPPFDAEAARGFISALMDLSFKSFVTPKVIQI
                     VYVLLLGVAALWTIAVIAAAFSAGTGIGILMLLLSPVLYLLCVLFIRMYLEIVIVLFR
                     IYEILRDRPLQT"
     gene            complement(2871..>3720)
                     /locus_tag="VE646_00615"
     CDS             complement(2871..>3720)
                     /locus_tag="VE646_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZD78532.1"
                     /translation="AAADTLLPANGTRLWVHREGQGPPLVVVHGGPVLDQTYLRGPLA
                     PLGRDAELVYYDQRLSGRSDGVVDSASVRLNVFVDDIEALRTTLGLGRIDLLAHSWGG
                     LLALRYALRYPEHLGRLVLVSPMAPSGALWQEEQRALQASLQPADTAGMGELGASPAV
                     AAGEPDAIRAMLRLSFRSELHDPELARTLDFHIEPDYRARSRQFGYLLPELLSYDMTG
                     RLGEVEAPTLLIFGQEEPGGSIGGEVLRRGIPDVTSVTIPDTGHFGFVEAPERFIEVV
                     RAFLAG"
BASE COUNT          524 a         1324 c         1342 g          530 t
ORIGIN      
        1 gtcatctggg tcaaccggac atacgtccgg gaggtcttcg gcgacgtcga tcccatgggt
       61 cagcgggtct acgcgggcaa cgagttccgc cgggtcgtgg gcgtcgtcga cgacgttccc
      121 tacgacgccg aaggcgacac ctcccgcaag ctgtacattc cccatgcgca gttcgcggac
      181 aaccggaact gggcgctcat ccagacggtc aaggtccggg cgggagcggc gtcgtcccgg
      241 gaagccatcc gcaaggtgct ggcgggtatg gacgggcagc tcgtgctgta ccatccggag
      301 atgttcgccg acgtggtggc cggcgcccgg gcgcaggacc gcttcgccac cgtgctcatg
      361 gccgccttcg cgctgctggc cctggcgctc tccctggtgg gcacctacgg ggtcctggcc
      421 ggcagcgtcg cgggacgcac ccgggagctc gggattcgca tggcgctggg cgccgatgcc
      481 cgctccgtgc gccggctggt gctccgctac gcgacggcgc tgatcgctcc gggcgtgctc
      541 atcggcctgg ccggtgcgtg gagcggcacc aggtggctac gcgccctcct cttccgggtg
      601 gaatcggggg atcccctcgt gtacgcgggg gtggtggcgc tgttcgccgt cgtcggcctg
      661 gtcgccggat gggctcccgc ccgcagggcc acgcgggtgg atcctgcgcg ggcgctgtcg
      721 atggagtaga gccgcaccct ctcctcccct cgccacctcc cgacgccacc tcccccgccg
      781 aagatcacgc cccgggtggt cccccgacgg cagccggatc gagtccctgg cgaccctgac
      841 ccgtcgcccg gccagcggtc catggccggg cccgggcacc ccgcggtaac atgaacggag
      901 gcctccgccg atcagcccgg agggagaccg tctcccggca ccggcaccca tcctgcatgg
      961 tcgtccgacg gacccggccg gtcatggcga aggacgcgct cgacgaggac ccgatggggg
     1021 cgtcacactc cgaaaggtat gaccacgtgg tggaccggtt cacagagctg gaggcggccg
     1081 ttgcgggggg gcgagtgacc cgcgtgaggg cagccggcgc ccgggacctc gaggggcggg
     1141 tcctccggac gttgcgtgcg ctcggggggg aggccacgcc cggggatctg gtcgcgatcc
     1201 tcgcccaccc tcgatccgac gtggaggcgg cgctggaggc gctgatcgcg tcggaccggg
     1261 tccaggtgaa ggtcctggac ccgggagacg tcgtctaccg gctggccggc gccggtcctc
     1321 acctggcccc ccgctccggc ctgtccgcct ctccccgtcg agccctgtcg ggaccggatc
     1381 gggcgccagc ggcggtgcgg ttcgaccgga agaccctcca cctcatccgg agcctgaagg
     1441 gcgtcatcag cctcgccgag ctggtcgagc acacgggcac gtcggtggcc gaggccaacc
     1501 gggagatgca gcggctcgtg agctggtacg gtggggaggg tcacccctcg ctcgacggcc
     1561 acgtggtcta cgccttcccg gggctcctga ccagcgtcca cacgccgccg gggacgcgcg
     1621 caccccgtcc cgcctggatg cgctggggcg atcccatggg gagccggagg ctcacggaga
     1681 gcggatcctg ggtgggccgt ctcgcctact ccctcggcct gctcggcttc ggccgtggcc
     1741 tcgtccgccg cgtcggggcc gtccgggcga tccgcttccg gcagcggtcc gtgctccggc
     1801 gcctggccct cggccacgtg ttccaggccg cgctggcggg atcgggtgtg gtgtcggtgg
     1861 gaagcgccag gcgctacctg gctttacgga ccggctaccg gatcggccgc cgtcgggtcg
     1921 aggcggtcct gcgggagttg tccggcgagt tcgacgcgcc ggtcacccgg gaaggcggag
     1981 agctgttctt cgggttccgg aacgtgaagc gccagttcct ggccgccgag ctcgtgcgcc
     2041 gccgactggg cctcgggcgt cgcgtcaccg ggcgaacggt cttcgacagc ggcgacactc
     2101 cacgggccgc agctcagcgc gagattgagg ctttcgatcg ggacctggcc ttcgcacact
     2161 ctcctcgccg caggacccct tcgtagcccc gacgccgggc cacccccttc agccctcgtc
     2221 tcccagcagc tctcgctccg ccaccatcct ggccagcgga ccggcggcgg aagcacagca
     2281 cggcatcgcg tggcgagcgt gcctccgcgg accgcggacc ccgcagatct caggtctgaa
     2341 gcggccggtc cctgagaatc tcgtagatac gaaagaggac gatcacgatc tccagataca
     2401 tgcggatgaa cagcacgcac agcagataca ggaccggcga caggaggagc atgaggatgc
     2461 cgatgcccgt gcctgccgag aaggcggcag cgatcacggc gatcgtccag agcgctgcga
     2521 cgccgagcag gagcacgtac acgatctgaa tgaccttcgg ggtgacgaag gacttgaacg
     2581 acagatccat cagcgcgctg atgaatcctc tggccgcctc ggcatcgaac ggcggctggc
     2641 ccgagggggt ggaacccgcg tcgtgctcgt tcactggact gcctcctggt tgggtgggat
     2701 tgcggggaat ctctcccgag gggagagtcc cggccagggt catcactcgc tcgagctcgg
     2761 ggcgtcagcc cagcagctcg gagacggtca cgaagcggta gccgcgcccg tgaagcaagc
     2821 tggtcccggc gctcccggca gcgtggagag gcaccgaatg accgaacgcg tcaccccgcc
     2881 aggaacgccc gcaccacctc gatgaagcgc tccggggcct cgacgaagcc gaagtgtccc
     2941 gtgtcgggga tggtcaccga cgtcacgtcc gggatccccc tccgcagcac ctcgcccccg
     3001 atgctccccc caggctcctc ctggccgaag atcaggaggg tgggcgcctc gacctccccg
     3061 agccgcccgg tcatgtcgta ggacagcagc tcgggcagca ggtacccgaa ctgccggctg
     3121 cgcgcccggt agtcgggctc gatgtggaag tccagggtcc gggccagctc gggatcgtgg
     3181 agctcggaac ggaacgagag ccgcagcatg gcccggatgg cgtcgggctc gccggccgcc
     3241 acggcgggac tggccccgag ctcgcccatg ccggcggtgt cggcaggctg gagcgacgcc
     3301 tgcagggccc gctgctcctc ctgccagagg gcgcccgaag gcgccatcgg gctgaccagc
     3361 accagccgcc ccaggtgctc cgggtagcgc agcgcgtagc ggagggccag caggccgccc
     3421 cacgagtggg ccaggaggtc gatccggccg agccccagcg tggtccgaag cgcctcgatg
     3481 tcgtccacga aaacgttcag gcgaacgctg gcgctgtcca ccaccccgtc ggaccgcccg
     3541 ctgaggcgct gatcgtagta gacgagctcg gcgtcccggc cgaggggcgc cagggggccc
     3601 cggaggtagg tctggtccag cacgggcccg ccgtggacca ccaccagggg cggtccctgc
     3661 ccttccctgt ggacccagag cctggtcccg ttggccggaa ggagggtgtc ggcggcggcc
//
LOCUS       DASQQQ010000015        20608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_55948, whole genome shotgun sequence.
ACCESSION   DASQQQ010000015 DASQQQ010000000
VERSION     DASQQQ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 20608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20608
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55948"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VE646_00620"
     CDS             complement(<1..256)
                     /locus_tag="VE646_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD78533.1"
                     /translation="MSRTRVVIADDDVLLREGLAGLLGSRGFAVLGRAGDGAELLSLV
                     RDHAPDLAIVDIRMPPAHAIEGLEAARVIREELPKTAILVL"
     gene            complement(351..2249)
                     /locus_tag="VE646_00625"
     CDS             complement(351..2249)
                     /locus_tag="VE646_00625"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HZD78534.1"
                     /translation="MGLRGRMNTASVLLSVLVAGTFVALFLSVEDLRRTASLERHSQE
                     VLVAANQVERLVVDLQTGTRGFLLTGDETFLEPWRAARSSLAVRTGSLQDLVADNPEQ
                     TRRARTIADAAASYLADYSIPLVRGAVRDSSDAIATVAAGDGEARVATIRAEFDRLVA
                     AERFLSTTRHDASEAAATRAIGAAGGGLAGSVVLVLLFPGYLGRLIVRPVQAMGRTAE
                     RIAEGDLTARASEGGVGEVALLGRSFNAMVVSVERSQSELARVAEEQSALRHVATLVA
                     RGAPSEDVFAAVTERVTRLCSADFGHMIVFEPDGTVTGIAGWSSGEGDPVPMASHVPL
                     EGESVTASIFRTGRPSRMDSYADASGSIAASLRHQGIRSSVGAPIVVEGRLWGAVIAS
                     SKGTEPLPEDTESRLTAFTELIATAISNVQARTELAASRARIVAAADEARRRIERNLH
                     DGTQQRLVSLGLKLRTVEARLPSGLDHLRDEMAWVGEGVEDVLEELREISRGIHPAIL
                     SQGGLGPALRVLARRSPIPVELDLRGDARVSEVVEAAAYYVASEALANAAKHSSASVA
                     TLELDIGDALLCLSIRDDGLGGADPARGSGLIGLKDRVEALGGSISVSSPIGGGTSIT
                     AELPVGAP"
     gene            complement(2460..3110)
                     /locus_tag="VE646_00630"
     CDS             complement(2460..3110)
                     /locus_tag="VE646_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD78535.1"
                     /translation="MNEGSITVLLIDDHVVVREGLRLLLEREPGVEVMAEASSLAEAL
                     AVEEQPEVVLADLMLPDEHGRDVVVQLRERFPRSAILVLTMVDNALGVRQAFEAGATG
                     YLLKEAASEELVNAVRCVASGERYVQSPLRRALAERPTPPPRHAPIPEPRLSARELEV
                     LTPLAFGHTNAEVASVLSVSRRTVETHRANLQRKLRIRSRADLTRYAVDQGLITLG"
     gene            complement(3131..3583)
                     /locus_tag="VE646_00635"
     CDS             complement(3131..3583)
                     /locus_tag="VE646_00635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZD78536.1"
                     /translation="MRFLVWHLRPPELDHLGLIPAVQRLIEETQRELGIPVDMHAAFP
                     QQRVPPRVETAVYRIVQEALTNAARHAAASSISIVMVQEPGALTVIVEDDGRGFDPER
                     FASHRESHMGFIGMRERAEQLGGTLVIDAAPGKGTVVRSRIPFDRPAA"
     gene            complement(3557..4114)
                     /locus_tag="VE646_00640"
                     /pseudo
     CDS             complement(3557..4114)
                     /locus_tag="VE646_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
     gene            complement(4935..5774)
                     /locus_tag="VE646_00645"
     CDS             complement(4935..5774)
                     /locus_tag="VE646_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZD78537.1"
                     /translation="MSAPEGVRPAGFQAPPFDAVLDLCGDPVTAHLRALLGGNQYMVL
                     PDLFSSFLGSDPGAGPIFYETLPSGVLTEQLRLGGLHMGSLELRFTPDLIAAGPDALA
                     RLHREGLVESPAGYAGNVLALLVASGDPCDVGGMDDLGRAGLRVALPNPETEGIGGLT
                     LQALEAAGGSELRDRVFVHKRGIGETVLTSIHQRQAPAWLREGSIDVAVVWDTEARHL
                     VGAGVPVERVEIEALVNQRGHYAAAVVSRAPHHAAAGSLLRHLSGPDGASTYQRYGFS
                     TGT"
     gene            complement(5761..6129)
                     /locus_tag="VE646_00650"
     CDS             complement(5761..6129)
                     /locus_tag="VE646_00650"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HZD78538.1"
                     /translation="MSGVEETGAPERGVIVHLDEGDPAKQDAVIRNIANLVAELPVAS
                     SVELVVHGPGLDAVLADGEHGGRVRELIDRGVAVAACANTMRRRDIPAEALLPGVRIV
                     PAGIAEVVQRQWQGWAYVRP"
     gene            6302..6982
                     /locus_tag="VE646_00655"
     CDS             6302..6982
                     /locus_tag="VE646_00655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZD78539.1"
                     /translation="MLEGKDEGPLPGGARRGDALVSTNPTAGRRPGETMIPDATLHRR
                     LLTRDVSVLGEIYGDLGAPAYGLALKITGQPSLAQDAVQDAFLALWELPQAYDPSRGT
                     FRTFLLSMVHHRAVDIVRREQRQSARVARVQHLAETVSADVAEEVTEASWATQRRAQV
                     RRALAALPDHQRQVLELAYFRGYTQLRIAEEIGVPLGTVKTRTLAAMRRLRSELAADA
                     PEAREETG"
     gene            complement(6989..7288)
                     /locus_tag="VE646_00660"
     CDS             complement(6989..7288)
                     /locus_tag="VE646_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78540.1"
                     /translation="MFGTIGRAHIRPENKDKLIEALEDPAYQGVSGYRMSYLMFPENR
                     ENEVVIVAMFEDADSYWKNADDPAQDERYQRYRALLEDEPEWSDGEWIAAPEGSA"
     gene            complement(7417..9024)
                     /locus_tag="VE646_00665"
     CDS             complement(7417..9024)
                     /locus_tag="VE646_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD78541.1"
                     /translation="MTEHAAGYERRWWILAILCLSLLVIGLDNTVLNVALPTLVRDLH
                     ASDSQLQWIIDAYVLVFAGLLLTTGSLSDRFGRRMVLGLGLLVFGTGSVASAFAASAP
                     MLIATRALMGLGGAMIMPSTLSILTNVFPPEELGRAIGIWAGVSGLGIGLGPVVGGYL
                     LDHLWWGSVFLVNVPIVITAFVGGRLIVPNSKDPAAPRIDPLGAGLSIAGLVTLVYAI
                     IEAPNHGWTDGAVVAEFIAAGVILAVFVWWELASDHPMLNVRFFENPRFTAANISMTL
                     VFFAMFGSLFFLTQYLQFLLGYTPLQAGLRTTPVALVMIVVAPLAGLLVRRLGNKLLV
                     TTGMLVAGLGLFLMSRTTVGSGYGNLLPAIIVLVAGMSMAMTPATESIMGSLPKEKAG
                     VGSAMNDTTRQIGGALGVAVLGSIFSSAYASGFGAGLSRLPVAAAAQVKSSVGGAFAV
                     AARIGGTSGRALASAATSSFVHAMDRGLAVGAIIAVAGGLVALIWLPNRAEPDREELE
                     RMGEVYAGHHPHQATPDEALLDGAPSS"
     gene            complement(9065..9511)
                     /locus_tag="VE646_00670"
     CDS             complement(9065..9511)
                     /locus_tag="VE646_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZD78542.1"
                     /translation="MSVGYRIGEVAERASVTPRTLRYYEELGLLEPAGHSPGGARRYS
                     DADVDRVLRIRDLQELMGFDLHAIHRIVTAEARLQRLREEYRAGVDVARRREMIREAI
                     EINDELRTEVREKLGRIRDVLQDLNAKAARYRRLLQSPPGATGRLR"
     gene            complement(9508..11277)
                     /locus_tag="VE646_00675"
     CDS             complement(9508..11277)
                     /locus_tag="VE646_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015618725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD78543.1"
                     /translation="MDRRRAQGTLHRPDRYRWTALSNTTLGVFMALLDSSIVIISLPA
                     IFRGIHLNPLDPGNIGYLLWILMGYLVVTAVLVVTFGRIGDIFGRVRMYNLGFLVFTL
                     GSVALSLTWSTGPAGATELIVFRFVQGIGGALLFANSTAILTDAFPVEQRGMAMGVNM
                     IAGIVGSFIGLVLGGVLADVHWRLVFWINVPFGLFGTVWAYAKLREVGQRTPAKIDWW
                     GNLTFGAGLIMVLVGITYGIQPAGGHTMGWTSPFVLSMIVGGLALLVLFCWIELRVEQ
                     PMFDLRLFAIRPFTAGNAAGLLSSIGRGGLQFMLVIWLQGIWLPLHGYSFISTPLWAG
                     IYMLPITAGFLLAGPASGWMSDRYGARPFATGGMLLAAATFGALMLLPANFSYPLFAA
                     LLLLNGIGFGLFAAPNTTGIMNSVPARQRGQASGMRATFQNSGMLLSIGVFFSLMIVG
                     LAAALPSTMQGQLVAHDVPAATAAQVAHLPPVGSLFAAFLGYNPMKTLLAPVLSSLPS
                     GQAAYLTGKTFFPSLVSGPFIHGLRIAFTASLVMCLIAAWSSWLRGAKFVHEEAGVGP
                     FGAPEHADAEVAEAPIPEEEMPA"
     gene            11433..12338
                     /locus_tag="VE646_00680"
     CDS             11433..12338
                     /locus_tag="VE646_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78544.1"
                     /translation="MLRILVAARDGLRTFDPVGVPGPVHHAGRAVTAVAPSRGQVWAI
                     LGGSEVWHSADLRDWTHVADLQGHRGTCITAVDDDVFVGSSEAHLFRVEGARLEAVSG
                     FDRAEGRSAWYTPWGGPPDTRSIANWDETTYVNVHVGGILRTEDRGRTWTPTIEIDAD
                     VHHVTTAEGSVLAACARGLAISTDRGLTWTLRRDGLEAPYSRAVAICGDAVLLSASRG
                     PRGGGAAVYRGGLSTDALERCRAGLPEWFDDNIDTYCLDAVPDSSFTAFGTADGRLFG
                     SHDGGGTWAELAAGLPPVTRVLLLP"
     gene            12421..12678
                     /locus_tag="VE646_00685"
     CDS             12421..12678
                     /locus_tag="VE646_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78545.1"
                     /translation="MAEKLVLVCDVCGKTPAGTVRFQVGRRIRLKDVCEEHLAELLKG
                     SRAPKPGRRPGAKAKAAKARTAKPQTASKRTPGRRRTKATG"
     gene            complement(12770..13360)
                     /locus_tag="VE646_00690"
     CDS             complement(12770..13360)
                     /locus_tag="VE646_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78546.1"
                     /translation="MMRRIATMLGTVALGLSLVIPATPALAGTSATSTIVRRVRPIGA
                     DGTLEPDYRVVRRARGRCWTSSIASQQRVAFRCLQDNLILDPCFASTPHVVYCMKDPW
                     THRVVRLRIRRLPSEHNAGIRRLRTPWGMELAGGRHCVLGTGTAAWVGRTALPYICSL
                     GYATEPRLSSTTRWHVRVAPEELDRLRRRAVAVALF"
     gene            13606..15279
                     /locus_tag="VE646_00695"
     CDS             13606..15279
                     /locus_tag="VE646_00695"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HZD78547.1"
                     /translation="MVRRYTRARRRYLPRALVLLVALALGFGTAVASAEEVPQPVDFV
                     HNVVDAPAPVPGAVFGTGPAVKTGHAICTTDTSTAANVNTDCSPTAGPHNETSIAVNP
                     TDPDNMIGGANDYQLGLNPGGHVSETILSRAHVTFDGGETWSEYPLYSSSTYQATGDP
                     AVAFDASGHAYYATLGFRFAGPVNGTNPDILVANSPDGGKTWKVVRVAKGSGTFLSVG
                     KSLDKEYIAAWGDGNAIVTYGNFLQGPHGALISARIYASVTHDHGTTWSRPQVISGSL
                     DQAFASVPTVTADGRIFVAFMNTSYLDPSSPDFTRDDYEVVQVDPDTGTALGSPVMVA
                     KVYDGLCDYPVALGRTTYQDSLFRTWAAGNITADPTDPDHLAVVWSDMRNTSGIPFDS
                     CDLFANPPEFADPYAVTTNSDVIVSESTDGGGSWSTPTAVTLAGDQFMPWGAFDTSGT
                     LRIGTFDRSYDPANHMYGYTLLTQAGAGFSGEQVTTELSDPTMNDRWFARTVNEDYPF
                     ATAFLGDYSNIAATSDGGVVAYWTDMRDDVTFAGRTGHDENAYFAKIAP"
     gene            complement(15445..16620)
                     /locus_tag="VE646_00700"
     CDS             complement(15445..16620)
                     /locus_tag="VE646_00700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017949279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZD78548.1"
                     /translation="MEILAGFALASLLGWLYLLLGHGRFWRTDQRLPHAPDPDRWPEI
                     IAVVPARNEASMLPRTLPALLAQRYPGVLRVIVVDDDSTDGTGELAAMLPGSAGDGAR
                     LTVIEGVPTPPGWAGKVWAMHQGLSLADGEAFVLFCDADVALGPDVLARLVRAAEGRG
                     LDLVSQMAELHAGTPWERVIVPAFVYFFAQLYPFRWVNRPVRRTAAAAGGCMLVRTAS
                     LRSAGGLEAMRDALIDDVTLARSLAGRPGGARLWLGHGGPGVTSVRPYPRLGDLWDMV
                     ARSASTQLRHSFPLVVATVAGMLLLYVVPPVAAVSGLTTAGIAGWSASWVGAVAGLAA
                     WAVMAATYVPTLRPYGLGRARALTLPGVATLYSAMTVDSYRRHLRGMGGAWKGRTAP"
     gene            16718..17086
                     /locus_tag="VE646_00705"
     CDS             16718..17086
                     /locus_tag="VE646_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78549.1"
                     /translation="MADIVEEGTFDPSSFRAELDAAAGNLQVGTSLWFANDRVRVWEV
                     RLEPGQRGPFHAHTRPYFWTCVGAGTGRQRSPDGTVRVRRYGEGETQYSEHSPTEPMI
                     HDLENAGPTILRFVTVELLA"
     gene            17194..17715
                     /locus_tag="VE646_00710"
     CDS             17194..17715
                     /locus_tag="VE646_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZD78550.1"
                     /translation="MGFQVAKVLDVAEGVQPDQFMVGERTELSSLDDLDPIYRRLLDE
                     PVTAVLAVTGGDGRANLTPVWFQYGDDKVLLNFAEHRRKTDWVRRDPHVTLLLVNPQN
                     AYHWISIRATVSREIHEDDPELGHLATETIDRAWTKYTGAEPPYGLRDPSRNERRVLF
                     ECAVERVATFGRP"
     gene            17750..18937
                     /locus_tag="VE646_00715"
     CDS             17750..18937
                     /locus_tag="VE646_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014174180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZD78551.1"
                     /translation="MSGWPSVAVAGGSLGGLTAALVLRDLGCDIHVYERATSELESRG
                     AGIVVLDETSRYLRERTDVDLGRVTVSTTWLRYLDRDGATVHREHRPYRYSGWHTLYR
                     TLLGRFDAERYHLGREMVDFVQDDRSVQVRFADGDTIGCDLLVCADGIGSSARARLLA
                     AARARYAGYVAWRGIVPEERVDPEARAAFADTLVYQVMPHSHILVYPIPSLEGETQPG
                     RRLLNFVWYRNVAEGAELEDLMTDRAGVVHETTLPPGAAREEHERAMRRLAERSLAPP
                     LAAVVEATTQPFVQVIFDIEVDRMAFGRICLIGDAAFALRPHIAAGTAKAAADAWALG
                     DALAGAGGDVPEALEAWEAGQLELGRTALQRARRNGDRSQFECTWRPEDPELAYGLWG
                     PGR"
     gene            complement(18910..20175)
                     /locus_tag="VE646_00720"
     CDS             complement(18910..20175)
                     /locus_tag="VE646_00720"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008746086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HZD78552.1"
                     /translation="MSPSAALVDRLRAAPLIDGHNDLLWEMREARRKERVEPDVGPPC
                     LRFHTDLPRMAAGGVGGQFWSVYVPSDLPADLAVTRTLEQIEALLRLVRRHPDRLEVA
                     RSAAEVEGIAGAGRVASMLGVEGGHAIGGSLGTLRLLAALAAGYLTLTHNDDTPWADS
                     ATGHHPHGGLTRFGEEVIRELNRSSMLVDLSHVSDDTMRHALEVSEAPVIFSHSSARA
                     LCDVPRNVPDDVLERVGRTGGVVMATFVPWFLTPEGAELNRASWEEVARQRELHPDDP
                     GAVRAAMESWDRSAPVPSATVSDVADHIDHIRGVAGIDSVGIGSDFDGTDAVPVGLED
                     VSRYPALFAELEARGYRGPDLAKVAGRNLLRVLREAERVAGRLQAERAPSFATLDEFD
                     RDPPPTVGSSHRPPEPPRGAGQRPGPQSP"
     gene            complement(20239..>20608)
                     /locus_tag="VE646_00725"
     CDS             complement(20239..>20608)
                     /locus_tag="VE646_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZD78553.1"
                     /translation="GYTARRPMAELERLGMLDLAEEDREIAPGVRVVHAPGHTPGHRV
                     AVLGSGRDTLLLTGDLLHLPAQVSRPRAPSTHDDDPPEGCRSRVRLLELAEAGGWRMG
                     VSHFAGPFGAVRSGRWVGRG"
BASE COUNT         3234 a         7260 c         7224 g         2890 t
ORIGIN      
        1 acagcaccag gatggcggtc ttggggagtt cctctcggat gacacgggcc gcctccaggc
       61 cctcgatggc gtgagcgggc ggcatccgga tgtccacgat cgcgagatcg ggggcatggt
      121 cccggaccag cgagaggagc tcggcgccat cgccggcacg gccgaggacg gcgaagccgc
      181 gggatccgag gaggccggcc agcccctcgc ggagcaggac gtcgtcgtcc gcgatgacca
      241 cgcgggtgcg gctcaccgta gctgcgctcc ggaggttggg cgctcggaca gggctcgagg
      301 cagtcgccac cggcaccaga gatgcagccg caccgggggg cgggcaccga tcatggggcg
      361 ccgaccggaa gctcggcggt gatcgacgtc cctccaccga tcggactcga caccgagatg
      421 ctcccaccca gagcctccac gcggtccttc aagccgatca gtccggagcc tcgggccggg
      481 tccgcgccgc cgaggccatc gtcgcggatc gacaggcaga gcagggcatc cccgatatcg
      541 agctcgaggg tggccaccga ggcgctcgag tgcttcgccg cgttggcgag ggcctccgag
      601 gccacgtagt aggcggccgc ctcgacgacc tcggaaacac gggcgtcgcc acgcaggtcg
      661 agctcgaccg ggatggggga gcgccgggcg aggacccgga gcgccgggcc cagacctccc
      721 tgcgacaaga tggcgggatg gatacctcgg gagatctccc gaagctcctc gagcacatcc
      781 tccacgccct ccccgaccca ggccatctca tcgcgcaggt ggtccagtcc cgatggaagc
      841 cttgcctcga ccgtccgaag cttcaggcca agcgagacca ggcgctgctg cgtgccatcg
      901 tggaggttgc gctcgatccg tcggcgggcc tcgtcggccg cggccacgat cctggctcgg
      961 gatgccgcga gctccgtccg agcttggacg ttggagatcg cggtggcgat gagctcggtg
     1021 aaggcggtca gacgcgactc ggtatcctcc ggcagcggct ccgtgccttt cgacgaggcg
     1081 atcacagctc cccacaggcg cccctccacg acgatggggg ctcccaccga tgaccggatc
     1141 ccctgatgcc ggagggaagc cgcgatggag ccagacgcgt ccgcatagct gtccatcctc
     1201 gagggcctgc cggtacggaa gatcgaggcg gtcacgctct cgccctcgag cggcacatgg
     1261 ctggccatcg ggacgggatc accttccccg ctgctccatc cggcgatccc ggtgaccgta
     1321 ccgtccggct cgaacacgat catgtgtccg aagtcggccg agcagagcct tgtcacgcgc
     1381 tcggtgaccg ctgcgaacac gtcctccgag ggagctcccc gggcgacgag cgtggcgacg
     1441 tgtcgaagcg cggattgctc ctccgcgacc cgggccagtt cgctctggct gcgttcgacg
     1501 gagacgacca tggcgttgaa ggagcgcccc aggagcgcca cttcccccac cccgccttcg
     1561 gacgcacggg ccgtgagatc gccctccgcg atgcgctcgg ccgtcctgcc catcgcctgg
     1621 accggccgga cgatcaaccg gcccaggtat ccggggaaca ggagcacgag cacgaccgat
     1681 ccggccaggc cgccacccgc ggcgccgatc gctcgggtgg ccgccgcttc cgaagcgtca
     1741 tggcgggtgg tggataggaa ccgctcggcg gcaacgagcc gatcgaactc cgcccggatc
     1801 gtggccacgc gcgcctcccc atcgccggcg gccaccgtgg cgatggcgtc cgacgagtcc
     1861 cggaccgcac cccgcaccaa ggggatcgag tagtccgcca ggtaggaagc ggcagcatcc
     1921 gcgatggtcc gggcccggcg ggtctgctcg ggattgtcgg cgaccaggtc ctgcagcgag
     1981 cctgttcgaa cggcgaggga cgatcgggcc gctctccacg gttccaggaa ggtctcgtcc
     2041 ccggtgagga ggaacccgcg cgtgcccgtc tggagatcga ccacgagtcg ctccacctgg
     2101 ttcgcggcga ccaggacctc ctgcgagtgc cgctcgaggg acgccgtcct gcggagatcc
     2161 tccacggaga ggaacaacgc tacgaacgtg ccggcaacga ggaccgacag caagacggag
     2221 gccgtgttca tccgcccgcg gagccccatg ctcaccgacc ctcctgtccg acgacgccac
     2281 gagcggacgc gaacccgccg ggccgcgagg aaccggccga atcagatctc gctccgtcgc
     2341 ctactacggc ccatccctcg gccctcgccg gcacccgccg ggaaggcaac acggtcacca
     2401 tcccaccagc gcgatcgcat ggtcatcagt aagaggtacg tggatctgct gcccgccact
     2461 cagccgagcg tgatcaaccc ctggtccacc gcataccgtg tgagatccgc gcggctccgg
     2521 atccgcagct tcctctgcag gttggcccgg tgggtttcca ccgtgcgccg ggagacggac
     2581 agcaccgagg ctacctcggc gttcgtgtgg ccgaacgcga gcggcgtgag tacttccagc
     2641 tccctcgcag agagccgggg ttcggggatc ggggcatgtc ggggaggtgg agtcggtcgc
     2701 tccgcgagcg cgcgtcgaag aggcgactgg acgtacctct cgccgctggc cacgcatcgg
     2761 acggcgttga ccagctcttc cgaggcggcc tccttcagca ggtatccggt ggcccccgct
     2821 tcgaacgcct gacggacgcc gagcgcgttg tccaccatgg tgagcacgag gatcgcgctt
     2881 cgcgggaagc gctcgcgtag ctgcaccacc acgtcgcggc cgtgctcgtc cggcagcatg
     2941 aggtcggcca gaacgacctc aggctgctcc tctacggcga gcgcttcagc gaggctggaa
     3001 gcctcggcca tgacctcgac gccgggctca cgctcgagca ggaggcgcag gccctcacga
     3061 acgacgacgt gatcgtcgat caggaggacg gtgatggatc cctcgttcac cgacatcatc
     3121 gttcaccggc tcacgctgcc ggacgatcga aggggatgcg cgatcgaacg acggttccct
     3181 tccccggggc ggcatcgatc acgagcgtcc caccgagctg ttcggcccgc tccctcatcc
     3241 cgatgaagcc catatgcgac tcgcggtgcg atgcgaagcg ctccgggtcg aacccccgac
     3301 cgtcgtcctc gacgatcacg gtcagggccc ccggctcctg gaccatgacg atcgagatgg
     3361 agctcgcggc ggcatggcgt gccgcgttcg tcagcgcttc ctggacgatc cggtacacgg
     3421 ccgtctcgac ccgtggcggc acccgctgct gggggaaggc cgcgtgcatg tcgacaggga
     3481 tgcccagttc cctctgcgtc tcctcgatga ggcgttgcac ggctgggatg aggccgaggt
     3541 gatcgagctc gggcggtcgc aggtgccaga ccaagaaccg catcctcgag accacggcgt
     3601 cgacccggcg atggagcgct gcggtgcgct cggccatctc cccggtcatg tcctgttcga
     3661 tgtcacgggc gaagagcgag atggaggtga ggacctggcc cagatcgtcg tgcagctccc
     3721 gtgccacgtt gcggcgttcc tcttcctgaa ccgagaccag gcgcgccagc aggcccctcc
     3781 gctgcccatc cgcgcgctcg agcagtcgga ggctctcccg gacctcttcc tcggcgcgtt
     3841 tgcgttcgaa cgctgccccc agcacgttgg ctacggcctg caggaaatcg atcgaatgag
     3901 gctcgaacga gcgagggcgt cgatcgtgca ctcccaggac cccgatcggg ctctccacgg
     3961 atcccacgat cacactcacc ccactcacga tgtcgtggtc ctgcaacagc ggcggggcca
     4021 cgaatcgctg ctccatcgcg aagtcctcca cgaccaccgg ttcggatcgc aggagcgtgt
     4081 acccggcctg cgatcccttc ccggcccgga cctttggccg aaccgaccac cccctcgtgc
     4141 cagccgatgc cggcccggac gatcagctcg ggccggtccg ggaggagctc caggaccttc
     4201 accgtatcga cctcgagggt gcgggccacg gcgcgaaccg tctcctccag gaacacgttg
     4261 aggtccgtct ccgcgagggc tcgacgaccg agctcggcgg ccacggcttg ctcatccgcg
     4321 cgtcgctcga gctcccgctt ggccgccagg atctccgtgt cctcgacgac gacgactccc
     4381 acgccctgga tccggtcgtc cgcggtgcgg tgggggtaga agctgaccac ccggtgccgt
     4441 tccatgcccg ggtccgccca ggtgcgcccc gtcacctcct catcgacgag cggtcgccca
     4501 gagcagagca catctcgaag gatcggctcc aggtgcggtg ccagatccgg ggcgacttcg
     4561 ccgacggtcc gtccgatgat cccggaacgg gggagcccga tctggtcggc cagggcgccg
     4621 ttgatctcta cgtaacgcaa ctggtggtcg aggaaggtga gaccgatcag cgccgaatca
     4681 gcagcgtcgc gaacaggctg ggcgacccag cctccagcga ccgggcaacg ccgttcccga
     4741 ccacgtcccc tcccacggac cgccccgtga gggttgcggt catccgcgcc gtgcggcgcc
     4801 atgatagacc ggccgcaggt gccgtgccct ccgcaaccag ggtgcaccct gagatgcgag
     4861 cgaccggctc catcgatcat cgttggccga tccaccgctg cccacctcga ccgctggtgg
     4921 cctgggcagg acggtcacgt cccggtgctg aacccgtatc gctggtaggt ggaggcacca
     4981 tcgggaccgc tcaggtgtct gaggagcgag cccgcggccg catggtgcgg ggctcgggag
     5041 acgaccgcgg ccgcgtagtg gccccgttgg ttcaccaagg cctcgatctc cacgcgttcg
     5101 accgggaccc cggcgcccac gaggtggcgc gcctcggtgt cccacaccac ggccacatcg
     5161 atcgagcctt cccgcagcca ggccggcgcc tgccgctggt ggatggaggt gagcacggtc
     5221 tctccgatcc cgcgcttatg gacgaagacc cgatcccgga gctccgatcc ccccgccgcc
     5281 tccagcgcct ggagcgtgag cccgccgatg ccctccgtct cggggttcgg gagcgccact
     5341 cgaagaccgg cccggccgag gtcgtccatc cctcccacgt cgcagggatc gccggaggcc
     5401 acgagcagag ccagcacgtt gccggcgtag ccggccgggg actcgaccag tccctcccgg
     5461 tgcagcctcg ccagcgcgtc gggaccggcg gcgatgagat ccggcgtgaa ccgcagctcc
     5521 agcgagccca tgtgtaggcc gcctagacgc agctgctcgg tcagcactcc cgaaggcaac
     5581 gtctcgtaga agatgggccc cgctcccgga tcgctcccca ggaagctgga gaacagatcc
     5641 gggagcacca tgtactggtt gccccctaac agggcccgga gatgggcggt caccgggtcg
     5701 ccgcacaggt cgagcaccgc gtcgaagggc ggtgcctgga acccggcggg ccgcacgccc
     5761 tcaggggcgg acataggccc atccctgcca ctgacgctgg acgacttccg cgatgccagc
     5821 cgggacgatc cgcacgccgg gaaggagcgc ctcggcgggg atgtcccgcc gtcgcatggt
     5881 gttcgcgcag gccgcgacgg caacgccccg gtcgatcagc tcgcggacgc gcccgccatg
     5941 ctccccgtcc gcgaggacgg cgtccaggcc gggtccatgg accaccagct cgaccgatga
     6001 tgccacgggg agctccgcca ccaggttcgc gatgttccgg atcacggcgt cctgcttggc
     6061 gggatcgccc tcgtccagat gaacgatgac tccccgttcc ggtgccccgg tctcctcaac
     6121 cccagacatg gagacgccgt acccgaccca caggcggcca acacctacgg atcagctggt
     6181 cctcgtgccc ctacccggct ggccgggccg gcttcgtgcc cgctagccgg taccgaaggg
     6241 ttgcgccagc tccaccgacg aacgggcgct cccctcgatg gcctgcggtc gtccctgggg
     6301 gatgctcgag gggaaggacg agggaccgct ccccggaggg gcaaggaggg gcgatgcgct
     6361 ggtcagcacc aacccgactg cgggcagacg acccggcgag accatgatcc ccgacgccac
     6421 cctgcatcgc cgtctcctca cacgcgacgt ctccgtcctg ggtgagatct acggggatct
     6481 gggagccccc gcctacgggc tggccctgaa gatcaccggt cagccctccc tggctcagga
     6541 tgccgtccag gacgcgttcc tcgccctctg ggagctcccc caggcctacg atccctctcg
     6601 cggcaccttc cggaccttcc tgctgtcgat ggtccaccac cgggccgtcg acatcgtccg
     6661 ccgggagcag cgtcagagcg cgcgggtggc cagggtccag catcttgcgg agacggtctc
     6721 ggcggacgtc gcggaggagg tcaccgaagc ctcgtgggcc acccaacgcc gggctcaggt
     6781 ccgtcgggct ctggccgcgt tgccggacca tcaacggcag gtcttggagc tggcctactt
     6841 ccgcgggtac acccagctcc gcatcgccga ggagatcggg gtgcccctgg gaacggtcaa
     6901 gacccggacc ctggccgcca tgcgacggct ccgctccgag ctcgcggccg acgcgcccga
     6961 ggctcgtgag gagacgggct gaggaccctt aggcggatcc ctccggggcg gcgatccatt
     7021 caccgtcgga ccactcgggc tcgtcctcca ggagggcccg gtagcgctgg tagcgctcgt
     7081 cctgtgccgg gtcgtcggcg ttcttccagt acgagtcggc gtcctcgaac atcgcgacga
     7141 tcaccacctc gttctcccgg ttctcgggga acatcaggta gctcatccgg tagcccgaga
     7201 cgccctggta cgcggggtct tcgagggctt cgatcagctt gtccttgttc tcgggacgga
     7261 tgtgcgctcg accgatcgtt ccgaacatgg ctcccctcct cgcctcgatc atctcggacg
     7321 cttgtacacg ggcccccgga gttgggtcaa ggtgccggga tcggtcccgc tccgaggggc
     7381 cgtgccgagg atctcgcccg gggatgacga ggaggctcag gaggagggcg ctccatcgag
     7441 gagcgcttcg tccggggtcg cctggtgcgg gtggtgaccc gcgtagacct cccccatgcg
     7501 ctccaactcc tcgcggtcgg gctcggcccg gttcggcagc cagatcaggg cgaccaggcc
     7561 gccggcgacg gcgatgatcg cgcccaccgc cagcccgcga tccatcgcgt gcacgaacga
     7621 ggacgtcgcg gcgctcgcca gggctcgacc ggaggtgccg ccgatccgag cggccacggc
     7681 gaacgcgccg cccacggagc tcttcacctg agccgcggcg gccaccggca gccgggacag
     7741 cccggcgccg aagccggacg cgtaggccga gctgaagatg ctgccgagca ccgccacgcc
     7801 cagggcgccg ccgatctggc gggtggtgtc gttcatggcc gagcccacgc cggccttctc
     7861 cttgggcagg gagcccatga tcgactccgt ggccggggtc atggccatcg acatcccggc
     7921 caccagcacg atgatggccg gcagcaggtt cccgtacccg gatcccacgg tggtccggga
     7981 catcaggaac agccccagcc cggcgacgag catcccggtc gtgaccagca gcttgttgcc
     8041 gagtcggcgc accagcaggc cggccagggg ggcgacgacg atcatgacca gcgccaccgg
     8101 cgtggtccgc aggcccgctt gcagcggtgt gtaccccagc aggaactgca ggtactgggt
     8161 gaggaagaac agtgacccga acatggcgaa gaacaccagc gtcatggaga tgttggccgc
     8221 cgtgaaccgc gggttctcga agaaccgcac gttgagcatc gggtggtcgg aggcgagctc
     8281 ccaccacacg aacaccgcca ggatcacccc ggccgcgatg aactcggcga ccaccgcgcc
     8341 gtcggtccat ccgtggttcg gcgcctcgat gatcgcgtac accagcgtga ccaacccggc
     8401 gatcgacagg cccgccccca gcggatcgat cctgggcgcc gcggggtcct tcgagttggg
     8461 gacgatcagc cgtccgccca cgaacgccgt gatcacgatg gggacgttga ccaggaacac
     8521 cgacccccac cacaagtggt cgagcaggta cccgccgacc accggtccca gcccgatccc
     8581 gagcccggag acgcccgccc agatcccgat cgcccgaccc agctcctcgg gtgggaacac
     8641 gttggtgagg atcgacaggg tggagggcat gatcatcgcg ccgcccaggc ccatcagcgc
     8701 tcgggtggcg atgagcatcg gagccgaggc ggcgaaagca gaggcgacgg agccggtccc
     8761 gaagaccagc aggccgaggc ccagcaccat ccgccgaccg aagcggtccg acaggctccc
     8821 ggtggtgagc aggagcccgg cgaagaccag cacgtaggcg tcgatgatcc actggagctg
     8881 gctgtccgag gcgtgcaggt cccggaccag cgtggggagg gccacgttca ggacggtgtt
     8941 gtccaggccg atcacgagca ggctgagaca gaggatcgcc aggatccacc accgccgctc
     9001 gtagcctgcc gcgtgctcgg tcatcggttg catgcgcgct cctgtcccgt atcgatcggt
     9061 ggggctaccg gagtcgcccc gtggcgcccg gtgggctctg cagcaggcgg cggtaccgag
     9121 ccgccttggc gttcaggtcc tgcaggacgt cccggatcct gcccagcttc tcccggacct
     9181 ccgtccgaag ctcgtcgttg atctcgatgg cctcgcggat catctcccgc ctgcgggcca
     9241 cgtcgacccc ggcccggtac tcctcccgca ggcgttggag tcgggcctcg gcggtcacga
     9301 tccgatggat cgcatgcagg tcgaagccca tcagctcctg caggtcgcgg atcctcagga
     9361 cccgatccac gtcggcgtcc gagtaccgtc gggcaccgcc ggggctgtgg ccggccggtt
     9421 ccagcagccc cagctcctcg tagtaccgga gggtgcgcgg ggtgacgctg gcccgctccg
     9481 cgacctcgcc gatgcgatag ccgacgctca cgcgggcatc tcctcctcgg ggatcggtgc
     9541 ctcggcgacc tcggcgtcgg cgtgctccgg cgctccgaac gggccgaccc cggcctcctc
     9601 gtggacgaac ttggcccccc ggagccacga gctccaggcc gcgatcaggc acatcacgag
     9661 cgacgcggtg aaggcgatcc gcaggccgtg gatgaacggt ccggacacca ggctggggaa
     9721 gaaggtcttg ccggtcaggt aggcggcctg cccgctgggg agggaggaca gcaccggggc
     9781 cagtagcgtc ttcatcgggt tgtagccgag gaacgccgcg aacaggctcc ccaccggcgg
     9841 caggtgcgcg acctgggcgg cggtcgcggc cggcacgtcg tgggccacga gctgcccctg
     9901 catcgtggag ggcagtgcgg ccgcgagccc gacgatcatg agcgagaaga acacgccgat
     9961 cgacagcagc atcccgctgt tctggaacgt cgcacgcatc cccgatgcct ggccccgctg
    10021 tctggccggc acgctgttca tgatcccggt cgtgttcggc gcggcgaaca gcccgaaccc
    10081 gatcccgttc aggagcagga gggcggcgaa caaagggtag gagaagttcg ccgggagcag
    10141 catgagcgct ccgaacgtcg cggccgccag cagcatgccg cccgtggcga agggccgggc
    10201 gccgtaccgg tccgacatcc atcccgacgc cgggccggcc agcaggaacc cggccgtgat
    10261 gggcagcatg tagatcccgg cccagagcgg ggtgctgatg aagctgtagc cgtgcagcgg
    10321 gagccagatc ccctggagcc agatcacgag catgaactga agtccaccgc gcccgatcga
    10381 ggacagcagg ccggccgcgt tgccggcggt gaacggacgg atcgcgaaca gccgcaggtc
    10441 gaacatgggc tgctcgacgc ggagctcgat ccagcagaac agcaccagga gcgcgaggcc
    10501 cccgacgatc atgctgagga cgaacgggct cgtccatccc atcgtatgcc cgccggcggg
    10561 ctggatcccg taggtgatgc cgaccaggac catgatcagc cccgccccga acgtgaggtt
    10621 cccccaccag tcgatcttcg ccggggtgcg ttggcccacc tcgcggagct tcgcgtaggc
    10681 ccagaccgtc ccgaagagac cgaagggaac gttgatccag aacaccagcc gccagtgaac
    10741 atcggccagc acgccgccga gcaccagccc gatgaacgag ccgacgatcc ccgcgatcat
    10801 gttgacgccc atggccatgc cccgctgctc gacggggaag gcgtcggtga ggatcgcggt
    10861 ggagttggcg aacagcagcg cgccaccgat gccctgcacg aaccggaaca cgatgagctc
    10921 ggtggcgccg gccggtccgg tgctccacgt gagcgagagc gcgacggagc cgagcgtgaa
    10981 caccaggaac ccgaggttgt acatgcggac ccggccgaag atgtccccga tccgtccgaa
    11041 cgtcaccacc aggaccgcgg tgacgaccag gtagcccatc aggatccaca gcaggtaccc
    11101 gatgttcccg gggtcgagcg ggttcaggtg gatgccgcgg aagatcgcgg gcagcgagat
    11161 gatcacgatg gacgagtcga gcagcgccat gaacacgccg agcgtggtgt tcgagagcgc
    11221 ggtccacctg taacggtcgg gccggtggag cgtcccctgg gcccgtcgtc ggtccatccg
    11281 tgttcctccg tccatcgcgg tcgtcgggcc cacgatgggg tgcgggcccg atgctggcga
    11341 tggtacctga cgttgacgtg aacatcagcc atggggaggg cggggcgccg tgaggctgcg
    11401 atagccaccg gccccacgtg ccaagatggc gcatgctgcg gatcctcgtg gcggcacggg
    11461 acgggctgcg gacgttcgat ccggtcggtg tgccgggtcc cgtccaccac gccggccgag
    11521 ccgtgaccgc cgtggcgccc tcacgcggac aggtctgggc catcctgggg ggttccgagg
    11581 tctggcactc ggcggacctc agggactgga cccacgtggc cgacctccag gggcaccgag
    11641 gcacctgcat cacggcggtc gatgacgacg tgttcgttgg ctcgtccgag gcccacctgt
    11701 tccgggtgga gggggccagg ctggaggcgg tgtccgggtt cgatcgcgcg gagggccggt
    11761 ctgcctggta caccccttgg ggcggtccac cggacacgcg ctccatcgcg aactgggacg
    11821 agaccaccta cgtgaacgtg catgtgggcg ggatcctccg gaccgaggat cggggacgga
    11881 cctggacccc gaccatcgag atcgacgcgg acgtccatca cgtcacgacc gccgagggct
    11941 cggtgctcgc cgcctgcgcc cgagggctcg ccatcagcac cgatcgcggg ctgacgtgga
    12001 ccctccggcg ggacggcctg gaggctccgt actcgagagc cgtggccatc tgtggagatg
    12061 ccgtcctgct gtcggcgtcg cgtggcccac gtggtggtgg ggcggccgtc taccggggag
    12121 ggctgagcac cgacgcgctg gaacggtgcc gagcggggct gccggagtgg ttcgacgaca
    12181 acatcgatac gtactgcctg gacgccgttc cggacagctc gttcaccgcc ttcggcacgg
    12241 ccgatggacg gctgttcggt tcgcacgatg gtggagggac gtgggctgag ctcgctgcgg
    12301 gcctccctcc cgtcacccgg gtgctcctgc tgccgtgaga gcgatccatc ccgagaatgg
    12361 tgtctggcac cgccgttgtc cgtagagtga tggggcgcag agcgagagga gcggcaggcg
    12421 atggccgaga agctggtgct ggtgtgtgat gtgtgcggga agacgcccgc cgggacggta
    12481 cggttccagg tcggccggcg catccggctg aaggacgtct gcgaggagca cctggccgag
    12541 ctcctcaagg gatcgcgggc tccgaagccc gggagacgtc ccggcgcgaa ggccaaggcc
    12601 gcgaaggcgc ggaccgcgaa gccccagacc gccagcaagc ggacgccggg gcgtcgtcgg
    12661 accaaggcga cgggctgaga gagatccctt cgggtccgca tcggccgtct ccatgggccg
    12721 tctccatcgg ccgcggaacg ggggatgggg aaggctcggg gccgtcgagt cagaacaggg
    12781 cgacggcgac ggcgcggcgg cggaggcggt cgagctcctc gggcgcgacc cgcacgtgcc
    12841 accgcgtggt gctcgacaga cgcggttctg tcgcgtagcc caatgagcag atgtacggca
    12901 gggccgtgcg gcccacccat gcagccgttc cggtgccgag gacgcaatgt cgaccaccgg
    12961 ccagctccat cccccagggc gtgcggagac gtcggatccc ggcgttgtgc tccgagggca
    13021 gtcggcggat ccgcaaccgc acgacgcgat gggtccatgg atccttcatg cagtagacga
    13081 cgtggggggt cgatgcgaag cagggatcaa ggatgaggtt gtcttggagg caccggaagg
    13141 ccactcgttg ctggctggcg atcgaggacg tccagcagcg acctcgggct cgacgcacca
    13201 cgcgatagtc cggttccagc gtcccgtctg cgccgatggg gcgcacgcgc cggacgatgg
    13261 tggatgtcgc cgaggtgccg gccagggccg gggtcgcggg gatcacgagc gacaggccga
    13321 gcgccacggt cccgagcatg gtggcgatcc ggcgcatcac gctggcagct cccttcgaac
    13381 ggcctcgtca tcctggctcc ttgctccgtc gagcgacacc ccccgtcgga cctacgtcgt
    13441 tcgggccgta tccgggcgca ggttctgaga tgcccttcca gggaacagcc tgttgaaaac
    13501 tcaacgcgct ccgcgtctga ccagtagatt cgcgttctcc gtcggggctg gatagggtgc
    13561 aggggtccgt cacctccaaa ggcggaccgg gaaggaggaa acgccatggt gcgacggtat
    13621 acccgagctc gacggaggta cctgccccgg gcgctggttc tcttggtggc gctggccctg
    13681 gggttcggca cggccgtcgc ctcggcggag gaagttccac agcccgtcga cttcgtgcac
    13741 aacgtcgtgg atgccccggc gccggtcccg ggcgccgtct tcggcaccgg tccagcggtc
    13801 aagaccggtc acgcgatctg cacgaccgac acgtccacgg cggccaacgt caacacggat
    13861 tgctcaccga ccgccggccc gcacaacgag acctcgatcg cggtcaaccc caccgatccg
    13921 gacaacatga tcgggggagc caacgactac cagctcggcc tgaacccggg cgggcacgtg
    13981 tcggagacga tcctctcgcg ggcgcacgtc acgttcgacg ggggcgagac ctggtcggag
    14041 tacccgctgt actccagttc gacctaccag gccacgggtg atcccgcggt ggccttcgac
    14101 gcttctgggc acgcgtacta cgcgaccctc ggcttccgct tcgccggacc cgtgaacggg
    14161 acgaacccgg acatcctcgt ggccaactcc cccgacgggg ggaagacctg gaaggtcgtg
    14221 cgggtcgcga agggtagtgg caccttcctg agcgtgggca agtcgctgga caaggagtac
    14281 atcgccgcgt ggggcgacgg gaacgccatc gtcacctacg gcaacttcct gcagggaccg
    14341 cacggcgccc tgatcagcgc aaggatctac gcctcggtga cccatgatca cggcacgacc
    14401 tggagccggc cgcaggtcat ctccggctcg ctggaccagg cgttcgcgtc ggttcccacg
    14461 gtgacggccg acggtcggat cttcgtcgcc ttcatgaaca cctcgtacct ggacccgagc
    14521 agtcccgact tcacgcgcga tgactacgag gtcgtgcagg tggaccccga caccggcacc
    14581 gcgctcggat cgccggtgat ggtggcgaag gtgtacgacg gcctctgcga ctatccggtc
    14641 gcgctcggac ggaccacgta ccaggacagc ctgttccgga cctgggcggc cggcaacatc
    14701 accgccgacc ccacggaccc cgatcacctg gcggtggtgt ggtcggacat gcggaacacg
    14761 tctgggatcc cgttcgactc gtgcgatctg ttcgcgaacc ctccggagtt cgccgatccg
    14821 tacgcggtga ccacgaactc cgacgtcatc gtgagcgagt cgaccgacgg gggcgggagt
    14881 tggtcgaccc cgacggcggt cacgctggcg ggtgatcagt tcatgccgtg gggcgccttc
    14941 gacacgagcg gcacactgcg gatcggcacg ttcgaccgca gctacgaccc ggccaaccac
    15001 atgtacgggt acacgctgct gacccaggct ggtgcgggct tctcgggcga gcaggtcacc
    15061 accgagctgt ccgatccgac catgaacgat cggtggttcg cgaggaccgt gaacgaggac
    15121 tacccgttcg ccacggcgtt cctgggcgac tacagcaaca tcgctgccac ttccgacggc
    15181 ggcgtggtcg cctactggac cgacatgcgt gacgacgtca cgttcgccgg ccgcacgggc
    15241 cacgacgaga acgcgtattt cgcgaagatc gcgccgtagg cggtcgatcg ggaggcacga
    15301 ggtccggcgc gcccgtcggg gcgcgccgga cctcggcgtc caggcggaac gggtggctgc
    15361 gccgccgcgt ccccggctgg gccccggtca gcacggtacg cgtggtggac aggcacccgc
    15421 gtccaccgac ccgaaccgac cgcatcaggg agcggtgcga cccttccacg ccccgcccat
    15481 cccgcggagg tgccggcggt aggagtccac ggtcatcgcc gagtagaggg tggcaacgcc
    15541 cggcagggtg agcgctcggg cccgacccag cccgtacggg cggagggtgg gcacgtaggt
    15601 cgccgccatc accgcccagg cggccaggcc tgcgaccgcc cccacccagg atgcggacca
    15661 cccggcgatc cccgcggtcg tcaagcccga cacggccgcc acggggggaa cgacgtacag
    15721 gagcagcatc cccgccacgg tggcgaccac cagggggaac gagtgacgga gctgggtcga
    15781 ggcgctccgg gccaccatgt cccacaggtc accgagcctc gggtaggggc ggacgctggt
    15841 cacgcccggt ccgccgtggc ccagccacag ccgcgctcct ccgggtcgtc cggccaggga
    15901 tcgggccagg gtgacgtcgt cgatcaaggc gtcccgcatg gcctccaggc cgccggccga
    15961 gcggagggat gcggtccgta ccagcatgca gccccccgcg gccgcggcgg tgcggcgcac
    16021 gggccggttc acccatcgga acgggtacag ctgggcgaag aagtacacga aggccggcac
    16081 gatgacccgc tcccacggtg tccctgcatg cagctccgcc atctgcgaga cgaggtccag
    16141 cccccggccc tcggctgccc ggaccaggcg tgccaggacg tcgggcccca gcgcgacgtc
    16201 ggcgtcgcag aacaggacga aggcttctcc gtccgccaac gacagccctt ggtgcatggc
    16261 ccagaccttg ccggcccaac cgggcggtgt gggcaccccc tcgatcacgg tgagccgcgc
    16321 cccgtccccg gcggagccgg gcagcatcgc cgcgagctcc ccggtgccat cggtgctgtc
    16381 gtcgtccacc acgatcaccc ggaggacccc tgggtaccgc tgggcgagca gcgctggcaa
    16441 cgtgcggggg agcatcgacg cctcgttccg cgccggcacc acggcgatga tctcgggcca
    16501 gcgatcggga tcgggtgcgt ggggcagacg ctgatccgtc cgccagaagc ggccgtggcc
    16561 caggagcagg tacagccacc cgagcagcga ggccagggcg aagcccgcaa ggatctccac
    16621 gcctccagcc tggcagaagc ggaaaagaga cgtcgtgacg ggccgcacgg cacggtgcac
    16681 cgggccctgg gtatagtcct cgcgccaagg agggcggatg gcggacatcg tcgaggaagg
    16741 caccttcgac ccctcgagct tccgggccga gctggatgcg gccgccggga acctgcaggt
    16801 gggcacgtcc ctgtggttcg cgaacgaccg ggtgcgggtg tgggaggtcc ggctcgagcc
    16861 cggtcagcgt ggcccgttcc atgcgcacac ccgcccctac ttctggacct gtgtgggggc
    16921 cgggacgggg cgtcagcgct cgccggacgg caccgttcgg gtccgtcggt acggtgaagg
    16981 tgagacccag tactcggagc actcgccgac cgagcccatg atccacgacc tggagaatgc
    17041 cggcccgacg atcctgcggt tcgtgaccgt ggagctgctc gcgtaagcgg gatcgccggc
    17101 ccccgcccat cctcggtcct gcgcacccgc gacgctcgtg gtacataccg tgagccggcc
    17161 gggccggtcg tcgatcacgg aggaggggat ctcgtggggt tccaggtggc gaaggtgctg
    17221 gatgtggcgg agggggtgca gcccgaccag ttcatggtgg gggagcgcac cgagctgtcc
    17281 tcgctcgacg acctcgaccc gatctacagg cggctgctgg acgagccggt gacggcggtg
    17341 ctggccgtga ccggcggcga cggccgagcg aacctcaccc cggtgtggtt ccagtacggg
    17401 gacgacaagg tcctcctgaa cttcgccgag caccgtcgga agaccgattg ggtccgccgc
    17461 gatcctcacg tgacgctgct gctggtgaac ccacagaacg cgtaccactg gatcagcatc
    17521 cgcgccaccg tctcacgcga gatccacgag gacgatcccg agctcggcca cctggcgacc
    17581 gagaccatcg accgggcctg gaccaagtac acgggcgccg agcccccgta tggcctgcgc
    17641 gatccctccc gcaacgagcg tcgggtgctg ttcgagtgcg ccgtcgaacg cgtggccacg
    17701 ttcggccggc cctgagcgtg ctcggtcttc cgatccgcgc gtcgacggca tgagcggctg
    17761 gccctccgtg gccgtggccg gggggtcgtt gggcgggctc accgccgcgc tcgtgctccg
    17821 cgatctcggg tgcgacatcc acgtctacga acgggccacg tcggagctgg agagccgggg
    17881 ggcggggatc gtggtgctgg atgagacctc gcggtacctg cgcgagcgga ccgacgtgga
    17941 cctgggccgg gtgaccgtga gcaccacctg gctgcggtac ctggaccgcg acggggcgac
    18001 cgtgcaccgg gagcaccgcc cctaccgcta ctcgggatgg cacaccctct accgcacgtt
    18061 gctcgggcgg ttcgacgcgg agcgctatca cctcggtcgg gagatggtgg acttcgtgca
    18121 ggacgaccgg tctgtgcagg tccggttcgc cgatggggac acgatcggat gcgacctgct
    18181 ggtctgcgcg gacgggatcg gctcgagcgc ccgggctcgg cttctggcgg cggcccgagc
    18241 tcggtacgcg ggctacgtgg cctggcgcgg catcgtgccc gaggagcgcg tggaccccga
    18301 ggctcgtgcg gccttcgcgg acaccctcgt gtaccaggtg atgccgcaca gccacatcct
    18361 cgtctacccg atccccagcc tggagggcga gacgcagccg ggacgacggc tcctgaactt
    18421 cgtctggtac cggaacgtgg cagagggggc ggagctcgag gacctgatga ccgatcgagc
    18481 gggggtggta cacgagacca cgctgccgcc cggcgccgcc cgcgaggagc acgagcgggc
    18541 gatgcgccgg ctggccgaac gatccctcgc tccaccgctg gccgccgtgg tggaggccac
    18601 cacccagccg ttcgtgcagg taatcttcga catcgaggtc gatcgcatgg cgttcggccg
    18661 gatctgtctg atcggcgacg ccgcgttcgc cctgcgtccc cacatcgccg ccgggaccgc
    18721 caaggccgcg gccgacgcct gggcgctggg ggatgcgctc gcgggagcgg ggggcgacgt
    18781 tccggaggcg ctcgaggcct gggaggccgg gcagctggag ctcgggcgga cggccctaca
    18841 gcgcgcccgg aggaacggcg atcgctcgca gttcgagtgc acgtggcgac ccgaggatcc
    18901 ggagctcgcc tacgggctct gggggccggg acgctgaccg gcgcccctcg gtggctcagg
    18961 cggtcggtgg ctggagccca cggtgggcgg tgggtctcgg tcgaactcgt cgagcgtggc
    19021 gaaggacggg gctcgctcgg cctggaggcg gccggccacg cgctcggcct cgcgcagcac
    19081 ccggagcagg ttccggccgg ccactttggc caggtccggg ccccggtagc cacgggcttc
    19141 cagctcggcg aacaaggccg ggtaccgcga cacgtcctcg aggccaaccg gcacggcgtc
    19201 cgtcccgtcg aagtcgctgc cgatacccac ggaatcgatc ccggccaccc cacggatgtg
    19261 gtcgatgtgg tccgccacgt cggagaccgt ggcggagggg acgggggccg accggtccca
    19321 gctctccatc gcggcccgca ccgccccggg gtcgtccgga tggagctccc gctggcgagc
    19381 cacctcctcc caggaggcgc ggttgagctc ggccccctcc ggggtgagga accagggcac
    19441 gaaggtggcc atcacgaccc cgccggtccg gccgacccgc tccaggacgt catcggggac
    19501 gttgcggggc acgtcgcaca gcgctcgggc cgaggagtgg gagaagatca ccggcgcctc
    19561 cgagacctcg agcgcatgcc gcatcgtgtc gtcggagacg tgggagagat ccaccagcat
    19621 gctcgagcgg ttcagctccc ggatcacctc ctcgccgaag cgggtcagcc cgccgtgcgg
    19681 atgatgaccg gtggctgagt cggcccaggg cgtgtcgtcg ttgtgggtga gcgtgaggta
    19741 gccggcggcc agagcggcca gcaggcggag cgtgcccagc gagcctccga tcgcgtgccc
    19801 cccctccacg ccgagcatcg aagccacccg cccggcgccg gcgatcccct ccacctccgc
    19861 cgcgctccgg gccacctcca gccggtccgg atgccgccgg accagtcgga ggagcgcctc
    19921 gatctgctcg agcgtgcggg tgacggccag gtcggccggc agatccgagg gcacgtatac
    19981 cgaccagaac tgcccgccca cgccccccgc cgccatccga ggcaggtccg tgtggaaccg
    20041 caggcagggg gggccgacgt cgggctcgac gcgctctttc cgtcgagcct cgcgcatctc
    20101 ccacaacagg tcgttgtgcc cgtcgatcag cggcgccgcc cggaggcggt cgaccagggc
    20161 ggcggacggt gacatcgcgg ccatgctagc cggggccgag tgcttcgggc cggggccagc
    20221 cgcggttctg gggcgccatc acccgcgccc gacccaccga ccgctccgca cggctccgaa
    20281 cggccccgcg aagtgcgaca cccccatcct ccaacccccg gcctccgcca gctccagcag
    20341 gcggacacgg gagcggcatc cctcgggcgg gtcgtcgtcg tgcgtggagg gggcccgggg
    20401 cctgctcacc tgggcgggca ggtggagcag gtccccggtc agcaggagcg tgtcccggcc
    20461 ggatccgagc acggccaccc ggtgcccagg ggtgtggccc ggcgcgtgga ccacgcggac
    20521 gccgggagcg atctcccggt cctcctcggc gaggtcgagc atgcccaggc gttccagctc
    20581 ggccatgggc ctccgcgccg tgtagccc
//
LOCUS       DASQQQ010000016         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_248920, whole genome shotgun sequence.
ACCESSION   DASQQQ010000016 DASQQQ010000000
VERSION     DASQQQ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248920"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VE646_00730"
     CDS             complement(<1..443)
                     /locus_tag="VE646_00730"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZD78554.1"
                     /translation="MILRTSECWLRGADGALLHFDNREAGPVGRGVCILVHGMGEHLG
                     KYEEWIGYALERGYHATGYDQRGHGRTPGQRGDFVFDDLVDDLGRCVGVSADLYPGMP
                     IFVLGHSLGALVALAYAGSGPHEALSGMALSGPLLAFAERVPIWL"
     gene            complement(440..1720)
                     /locus_tag="VE646_00735"
     CDS             complement(440..1720)
                     /locus_tag="VE646_00735"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HZD78555.1"
                     /translation="MTTGAKAPSEAEIIAIGNELLLGETVDTNSAHVARRLSQIGLRV
                     TRTTHAGDDAERLRGVLEEALARSRWVIAMGGLGPTRDDVTRPVVAEVIGRPLRIDPD
                     LLAEVEAKFRRFGYERMPPANRSQAEVPEGARTIPNPHGTAPGLVLEKDGTTLFVLPG
                     VPAEMKALLEDGVLPTIGGELGEDATVIRSRLVRTAGIGESALAEEIDDLVGNAGPIQ
                     VAFLPELGQVDIRLTAVGIPAEEADRRLEELATAIRRRVGRWFFGEGEITLAGALRDA
                     LRSRDWTLAVAESCTAGELAAEITSVPGSSDYFLGGVVSYANAVKASLLGVAPETLER
                     HGAVSEETCREMVAGVRERLEADVGAAITGVAGPGGGTPEKPVGLVHCGVGTPEGSCF
                     RRLDVPGSRDAVRRRATVATLSLLLRKVRGKEPS"
     gene            complement(1717..2154)
                     /locus_tag="VE646_00740"
     CDS             complement(1717..2154)
                     /locus_tag="VE646_00740"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HZD78556.1"
                     /translation="MSLARRVATVGLVGYWPWGPGTLASALVGLLWWTVSLPRWIWLT
                     AVAVLTVVGIAAAGQAERDLGRDDGRIVIDEVVGMGLALVAVSHTLAGTGLAFALFRA
                     FDILKPPPVAHLQRLPRGWGVVGDDLAAGALAAGGGWLVTGLW"
BASE COUNT          369 a          877 c          748 g          291 t
ORIGIN      
        1 tcgagccaga tcgggacgcg ctcggcaaac gccagcagag gaccggacag ggccatcccg
       61 gacagcgcct cgtgcggccc gctcccggca tacgccagcg ccaccagggc acccaggctg
      121 tgccccagaa cgaagatcgg cattcccgga tagaggtccg cgctcactcc gacgcagcgt
      181 ccgaggtcgt ccaccaggtc gtcgaagacg aaatcgccgc gctggcccgg ggtccggcca
      241 tgtccgcgct ggtcgtatcc ggtggcgtgg tagccgcgct ccagcgcgta accgatccat
      301 tcctcgtact tgcccagatg ctcgcccatc ccgtgcacca ggatgcacac gccacggccg
      361 acgggccccg cctctcggtt gtcgaaatgg agcaacgccc cgtccgcccc gcggagccag
      421 cactcggagg tgcgcaggat cacgacggct ctttcccgcg caccttgcgc agcaggagcg
      481 acagcgtggc cacggtggcc cgccggcgga cggcgtcccg ggacccggga acgtcgagcc
      541 ggcgaaagca gctcccctcg ggggtcccca ctccgcagtg gaccaggccc accggtttct
      601 ccggcgtccc acccccgggg ccggcgacgc cggtgatggc ggcaccaacg tcggcctcga
      661 ggcgctcgcg caccccggcc accatctcgc ggcaggtctc ctcgctgacg gcgccatggc
      721 gctccagcgt ctcgggagca acccccagca gcgacgcctt gacggcgttg gcgtaggata
      781 cgacccctcc caggaagtaa tcgctcgagc cgggcacgct ggtgatctcc gcagccagct
      841 caccggcggt gcacgactcc gcgaccgcca gcgtccagtc ccgggagcga agggcatctc
      901 tcagagcccc cgccagcgtg atctcgccct cgccaaaaaa ccagcgcccg acccgccgcc
      961 ggatggcggt cgccagctcc tccagccggc ggtcggcctc ttcggcggga atcccgaccg
     1021 cggtcaggcg gatgtcgacc tgacccagct cgggcaggaa cgccacctgg atggggcccg
     1081 cgttcccgac cagatcgtcg atttcctcgg cgagggccga ttcgccgatc cccgccgtgc
     1141 gcacgagccg cgagcggatc accgtagcgt cctcgcccag ttccccgccg atcgtcggga
     1201 gcacgccgtc ctcgagcagc gccttcatct ccgcgggcac ccccgggagc acgaacaggg
     1261 tcgtcccgtc cttctcgagc accagcccgg gggccgtacc gtgtgggttg gggatcgtcc
     1321 gcgcgccttc cggcacctcc gcctggctgc ggttggcggg tggcatccgt tcgtagccga
     1381 agcgccggaa cttcgcctcg acctcggcca ggagatcggg atcgatccgc agcggacggc
     1441 cgatgacttc ggccaccacg ggacgggtga cgtcatcccg ggttggaccg agaccgccca
     1501 tggcgatgac ccaacgtgag cgcgcgaggg cctcctcgag cacgccccgg agccgttccg
     1561 cgtcgtcgcc ggcgtgggtg gtccgggtga cgcggagccc gatctgggaa agccgccggg
     1621 ccacgtgagc gctattggtg tcgaccgtct cgcccagcag gagctcgttg ccgatggcga
     1681 tgatctcggc ctcgctgggg gctttcgcgc cggtggtcac caaaggcccg tcaccagcca
     1741 cccgcccccg gcggccaggg ccccggcggc gagatcgtcc ccgaccacgc cccagccgcg
     1801 ggggagccgc tgcagatgag cgacgggcgg tggcttcagg atgtcgaaag cacggaacag
     1861 ggcgaaagcc agccccgttc cggccagcgt gtgagatacg gccaccagcg ccagacccat
     1921 cccgaccacc tcgtcgatca cgatccggcc gtcatccctc cccaggtcac gctccgcctg
     1981 cccggcggcg gcgatcccca ccacggtcag gacggcgacg gcggtgagcc agatccaccg
     2041 cggcagcgaa accgtccacc agaggagccc caccagcgcc gacgccagcg ttcccggccc
     2101 ccacggccag tacccgacca gcccgacggt ggccacccgg cgggcgaggc tcacgtccgc
     2161 gcttccgaca ggacatcccg gtactggacc aggtagtcca cgagcgaaag aacggtcagc
     2221 accaccacga accagaagga gacgttgacg acctccagcg tatagccctg ccagtgggtg
     2281 aagaa
//
LOCUS       DASQQQ010000017         6587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_56002, whole genome shotgun sequence.
ACCESSION   DASQQQ010000017 DASQQQ010000000
VERSION     DASQQQ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56002"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VE646_00745"
     CDS             complement(<1..844)
                     /locus_tag="VE646_00745"
                     /inference="COORDINATES: protein motif:HMM:NF039285.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HZD78557.1"
                     /translation="MIDRERRSISTGLPLAAFLASLMGSVGLVVIYVLGGRPPWEGAF
                     LGLALGGVGLGLVLWAKRFLPVGHDVERRTPLPSPAPKLAEAVDAFERGAEMMTRRRV
                     LLSALGAALGAMGLAALFPIRSLGRAPKGALFHTEWRAGLRAVNVNGTAVKATDVVPG
                     NVITVFPEGSVGSADSQTLLIGLEPGLYRPLPGREDWAPDGIVGYSKVCTHAGCPVGL
                     YQASLHRLFCPCHQSIFDVLEGATPIGGPADRPLPQLPLQVDDEGYLVAQGDFPEPIG
                     PGFWE"
     gene            complement(841..1653)
                     /locus_tag="VE646_00750"
     CDS             complement(841..1653)
                     /locus_tag="VE646_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZD78558.1"
                     /translation="MPGRVLRLLLMAGALGLATAGCTYFVQRGPSPYRPPGLYPTVSE
                     ADTGRILYDRDCAWCHGPHGEGTNNGPNLNGALDGGAYTDFMLRTNRMPLTAPGATSR
                     TQPSPYTDGEISSIVSYVETFGGTGPPVPSPDPAAGDLNSGAELYLENCAACHSTTGI
                     GEALTSGQVAPNLRPARAPQIAEAVLVGPGCPNTSRACGPGEGAMPRFDFTQGQMDSL
                     VRYVQYLQDPRDRGGASLGHLGPVAEGAIALVVGLGIMLAVTRWIGTRVGEG"
     gene            complement(1653..2528)
                     /locus_tag="VE646_00755"
     CDS             complement(1653..2528)
                     /locus_tag="VE646_00755"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HZD78559.1"
                     /translation="MTPMVGHALVGPGELWGAWSPDPTVLAGLGAAGLLYARGYRTIR
                     RRSPHPTADLRAGAFLAGWLVIVVALVSPLDAMADALFSAHMVQHLLLIVVAAPLFVL
                     GRPVITTLAGLSPERRRRVARAAHALAATGVVRTVRRPVVAWSVFTIALWSWHLPVLY
                     QAAVEDPLLHALEHTAFLSTAMLAWSVALDERPREGMGVLGRALFLSASAVQSGLLGA
                     LILFASTLLYPVHGPGPALWGLTPLQDQQLAGALMWIPPAAVYLTATGAILLRTFRAM
                     DASRPARRAGGSVAA"
     gene            complement(2528..2941)
                     /locus_tag="VE646_00760"
     CDS             complement(2528..2941)
                     /locus_tag="VE646_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78560.1"
                     /translation="MSQTPTQNEERIRRKDHRSVLAWIGVLTGPVAWVLQLYWSWAIG
                     EFLACGPASRAGDRIFGFDMNTFSAMLNGGLLAFTVVAGVLSYVELRSLRSRRDRTPA
                     DRATWLARSGIMSSILFAVLIAVSFVPVVMIGGCG"
     gene            complement(2951..3580)
                     /locus_tag="VE646_00765"
     CDS             complement(2951..3580)
                     /locus_tag="VE646_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HZD78561.1"
                     /translation="MSEARAFEPTGRGAEVLPTTALGSRSPSWWGMVWLIATEAVLFS
                     MLLMSYWFLRFQSTPVWPPDGIDKPKLTLVLIMTAILWSSSLPVHLAHRAVRLGHQVA
                     AQFWMLIGWFPGLLFVALELGVEWPHTLKEFNPQTNAYGSLYFTITGFHALHVIIGLM
                     VSAWVQVRLWRGAYDMHRHVSVQNFSMYWQFVDLVWVFVLLTVYLSPYL"
     gene            complement(3577..5460)
                     /gene="ctaD"
                     /locus_tag="VE646_00770"
     CDS             complement(3577..5460)
                     /gene="ctaD"
                     /locus_tag="VE646_00770"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HZD78562.1"
                     /translation="MASIAERLDEVWGETPGLASWIGTVDHKRIGMRYLYTGFVFFAI
                     AGVGAMLLRVQLTRPGLQVLSPEAYDQLFSMHGVTMMFLFATPVLSGFGNYFVPLMVG
                     ARDMAFPRLNAFGYWVFLGAGIFMTSAFISGQAPNAGWFDYVPLASTAFTPEHGIDFY
                     ALGLIFITISTTTGAINFIVTTFKMRAPGMTLNRIPIFVWGELAFAFTAVFALPALSV
                     ANVFLELERKFGFHFYDAGNGGNPILWQHLFWIFGHPEVYLVILPALGLVSAIVPTFS
                     RRPMIGYTYIVLAELFIFVISFGVWVHHMFAVGLPMIAMGFISAASFLVVVPSGTQIF
                     AWLATMVSGRLVLKTPMLFVLGFLFVFVVGGLTGAMFSAVPFDQAITDSYFVVAHFHY
                     VMAGGAFFPMFAGLYYWGPKMLGRKLHERAGQISFWLMFIGFNLTFFPMHLAGLAGQP
                     RRTYTYQPGLGWGVYNLWETVGVFILALGILLTFINWFWARSHGEPAGDDPWDGETLE
                     WTISSPPPEYNFETVPEVRSREPAWDQPELHRGFQSPEEGGRPLTSGHLTLATSVSDA
                     EPEAVVKMPHESPWPFIVSVALLVSFYAFLLSQWGLFAFGMVAVAGSVMGWMWPRGET
                     QET"
     gene            complement(5460..6368)
                     /gene="coxB"
                     /locus_tag="VE646_00775"
     CDS             complement(5460..6368)
                     /gene="coxB"
                     /locus_tag="VE646_00775"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HZD78563.1"
                     /translation="MLDAHGASEKRVEGLWWLLFAISAVVLAVVLGLMTAAIIRRRRR
                     GTDVDRSEPRWGEPFVVLAGVIIPVLILTAVFVVSVRDMNAIAGGDPQLTVQVIGHMW
                     WWEARYPNGAITANEIHIPAGEPVRIEVTTADVIHSFWVPQLAPKIDMIPGKTNSIVL
                     VADSPGRYRGQCAEFCGLQHAHMAFFVVADPPDVFQRWMAHQEAPAAAPTGSAADGLA
                     VFLAQTCDGCHTIRGTPASAALAPDLTHLADRETIAAGTLRLTRGNLAHWIRDPQAVK
                     PGVIMPPTQLTRSQLQSLVDYLMGLN"
BASE COUNT         1173 a         2331 c         2238 g          845 t
ORIGIN      
        1 tctcccagaa ccccggcccg atcggctcgg ggaagtcccc ctgtgccacc aggtacccct
       61 cgtcatccac ctgcaggggc agctggggca atggccggtc ggccggccct ccgatgggcg
      121 tggcgccctc cagcacgtcg aagatcgact ggtggcaggg gcagaagagc cggtggaggc
      181 tggcctggta caggcccacc gggcacccgg cgtgggtgca gaccttcgag tagccgacga
      241 tgccgtccgg ggcccagtcc tcacggccag gaagcggacg gtacaggccc ggctccagac
      301 cgatcagcag ggtctgggaa tccgctgagc ccacggatcc ctcggggaag accgtgatga
      361 cgttgccggg cacgacatcg gtcgccttga ccgccgtccc gttgacgttc acggcccgta
      421 gacccgcccg ccactccgta tggaacagcg ctcccttggg ggcccgcccc agcgagcgga
      481 tcgggaacag ggccgccagc cccatcgccc ccaacgcggc gccgagggcg ctcagcagca
      541 cgcgtctgcg ggtcatcatc tccgcgcccc gctcgaaggc gtccacggcc tcggccagct
      601 tgggagcggg cgagggcagg ggtgtccgcc gttcgacgtc gtgtccaacc ggcaggaaac
      661 gcttggccca gagcaccagg cccaggccga cgccccccag cgccaggccc aggaacgctc
      721 cctcccacgg gggcctcccc cccagcacgt agatcacgac cagtcccacc gagcccatga
      781 ggctcgcgag gaacgccgcc agcggcagac cggtcgatat cgagcgacgc tctcggtcga
      841 tcatccctca cccaccctcg tgccgatcca tcgcgtgacc gccagcatga tgcccaaccc
      901 caccacgagc gcgatcgcgc cctcggccac cgggcccagg tgcccgaggg acgccccacc
      961 ccggtccctg gggtcctgca ggtactgcac gtaccgcacg agcgaatcca tctgtccttg
     1021 ggtgaagtcg aacctcggca tcgccccttc ccccggtccg caggcgcggc tggtgttcgg
     1081 gcagccgggc cccacgagca cggcctcagc gatctggggg gcccgcgcgg ggcggaggtt
     1141 gggagccacc tggccggagg tcagcgcctc cccgatgccc gtggtcgaat gacaggcggc
     1201 gcagttctcc aggtagagct cggcccccga gttcaggtca ccggccgccg ggtccggcga
     1261 gggcaccggg gggccggtcc cgccgaaggt ctcgacgtag gagacgatcg aggagatctc
     1321 accatccgtg tacgggctcg gttgggtccg ggaggtcgcc ccgggggcgg tcagcggcat
     1381 gcggttggtc cgcagcatga aatcggtgta cgccccgccg tccagggccc cgttcaggtt
     1441 cggcccgttg ttcgtgccct cgccgtgcgg gccgtggcac cacgcgcagt cacggtcgta
     1501 gaggatccgg ccggtgtccg cctcggagac ggtcgggtac aggccgggcg gacggtacgg
     1561 gctcgggccg cgctggacga agtacgtgca tcccgccgtc gccaggccca acgctccggc
     1621 catcagcagc agacggagga cgcgcccggg catcaggccg ccacgctccc ccccgcgcgc
     1681 cgggcgggcc gcgaggcatc catggcgcgg aaggtccgca gcaggatggc ccccgtggcc
     1741 gtgaggtaga cggccgccgg cgggatccac atcaacgctc ccgccagctg ctggtcctgc
     1801 aggggggtca gcccccacag cgcgggaccc ggtccgtgga cgggatacag gagggtggag
     1861 gcgaacagga tcaacgcacc cagcagaccg ctctggaccg cggaggccga gaggaacagg
     1921 gcccggccca gcacccccat cccttcgcgg ggacgctcgt cgagcgccac cgaccaggcg
     1981 agcatcgccg tggacaggaa ggccgtgtgc tcgagggcat ggagcagcgg gtcctccacc
     2041 gcggcctggt acaggaccgg caggtgccag ctccacagcg cgatggtgaa cacgctccag
     2101 gccaccaccg ggcgacgaac ggtccggacc acgcccgtcg cggccagggc gtgcgcggcc
     2161 cgggccaccc gacgtcggcg ctcgggcgac agacccgcca gcgtggtgat cacgggccgt
     2221 ccgagcacga agagcggagc ggcgacgacg atcaacagga gatgttgcac catgtgggcc
     2281 gagaacaggg catcggccat ggcgtccagc ggggacacca gcgccacgac gatcaccagc
     2341 cagcccgcga ggaaggcgcc ggcgcgcaga tcggcggtgg gatgtgggct ccgtcgccgg
     2401 atcgtccggt agccccgcgc gtacaggagg cccgcagccc ccaaccccgc caggaccgtc
     2461 ggatccgggc tccacgctcc ccagagctcg cccgggccga ccagcgcgtg acccaccatg
     2521 ggggtcatca gccgcacccc ccgatcatga cgaccggcac gaagctgacc gcgatcagga
     2581 ccgcgaacag gatgctcgac atgatcccgg agcgagccag ccacgtggct cggtcggccg
     2641 gcgtccgatc gcgtcgcgag cgcagcgacc gcagctccac ataggacagc acccccgcca
     2701 cgaccgtgaa ggccaacaga ccgccgttca gcatggcgga gaaggtgttc atgtcgaagc
     2761 cgaagatccg gtcgcccgcc ctggatgccg ggccgcaggc caagaactcg ccgatcgccc
     2821 agctccagta gagctgcagc acccaggcca ccggcccggt caggacaccg atccaggcca
     2881 ggaccgatcg gtggtccttc cggcggatcc gctcctcgtt ctgggtgggt gtctgggaca
     2941 tcggacgacc tcacaggtag ggagacaggt acacggtgag caggacgaag acccacacca
     3001 ggtccacgaa ctgccagtac atcgagaagt tctggacgct cacgtgccgg tgcatgtcgt
     3061 acgccccccg ccagagccgg acctgcaccc aggcgctgac catgaggccg atgatcacgt
     3121 gcagcgcgtg gaacccggtg atcgtgaagt acagcgagcc gtaggcgttc gtctgcgggt
     3181 tgaactcctt cagcgtgtgc ggccactcga ccccgagctc cagggccacg aacagcagcc
     3241 cggggaacca gccgatcagc atccagaact gggccgctac ctgatgcccc aagcggaccg
     3301 cacggtgcgc caggtgcacc ggcaggctgg agctccacag gatcgccgtc atgatgagga
     3361 ccagcgtgag cttcggcttg tcgatcccat ccgggggcca caccggcgtc gactggaacc
     3421 gcaggaacca gtacgacatc agcagcatcg agaacaggac ggcctcggtg gcgatcagcc
     3481 agaccatgcc ccaccacgag ggggaccggg accccagcgc ggtggtcggg agcacctcgg
     3541 ctccccgccc ggtgggctcg aacgcacgtg cctcgctcac gtctcctggg tctcccccct
     3601 gggccacatc cagcccatca cgctgcccgc gacggccacc atcccgaagg cgaacagccc
     3661 ccactggctc agcaggaacg cgtagaagga gaccaacagc gccactgaga cgatgaacgg
     3721 ccagggagac tcgtgcggca tcttcaccac ggcctccggc tcggcatcgc tcacgctggt
     3781 ggccagggtg aggtgccccg aggtcaacgg ccggccgccc tcctcagggg actggaagcc
     3841 gcgatggagc tccggctggt cccaggccgg ctcgcggctg cgcacctcgg ggacggtctc
     3901 gaagttgtac tcggggggcg gggagctgat ggtccactcc agcgtctcgc cgtcccaggg
     3961 gtcatctccg gccggctcgc cgtgggaccg cgcccagaac cagttgatga acgtgaggag
     4021 gatcccgaga gccaggatga acacccccac cgtctcccac aggttgtaga cgccccatcc
     4081 cagcccgggc tggtacgtgt aggtgcgacg gggctggccg gccaacccgg ccaggtgcat
     4141 ggggaagaac gtcaggttga acccgatgaa catcagccag aagctgatct ggccggcccg
     4201 ttcgtggagc ttgcggccca gcatcttggg gccccagtag tacaggccgg cgaacatcgg
     4261 gaagaaggcg ccgccggcca tcacgtagtg gaagtgcgcc accacgaagt aggagtccgt
     4321 gatggcctgg tcgaatggca cggcgctgaa catcgccccc gtgagaccac ccaccacgaa
     4381 gacgaacagg aagcccagca cgaacagcat cggggtcttc aggacgagcc ggcccgacac
     4441 catcgtcgcg agccaggcga agatctgcgt cccgctcggc accaccacca ggaagctcgc
     4501 cgccgagatg aaacccatcg cgatcatcgg cagacccacc gcgaacatgt ggtgcaccca
     4561 cacgccgaag ctgatgacga agatgaacag ctcggccagg acgatgtacg tgtagccgat
     4621 catcgggcgg cgggagaagg tgggcacgat ggccgagacc agccccagcg ccggcaggat
     4681 caccaggtac acctccggat gcccgaagat ccagaacagg tgctgccaga ggatggggtt
     4741 gccgccgttg cccgcgtcgt agaagtggaa gccgaacttc cgctccagct ccaggaacac
     4801 gttggctacc gacagggccg gcagcgcgaa cacggcggtg aatgcgaagg ccagctcgcc
     4861 ccacacgaag atcgggatgc ggttcagggt catgccgggg gcccgcatct tgaacgtggt
     4921 gacgatgaaa ttgatggctc cggtcgtggt cgagatcgtg atgaagatca accccagcgc
     4981 gtagaagtcg atgccgtgct cgggggtgaa cgcggtggag gccaggggca cgtagtcgaa
     5041 ccaccccgcg ttcggggcct gcccggagat gaaggccgac gtcatgaaga tgccggcccc
     5101 gaggaagacc cagtagccga aggcgttcag gcgcgggaag gccatgtccc gggcgccgac
     5161 catgaggggc acgaagtagt tgccgaaccc cgagagcacg ggggtggcga agaggaacat
     5221 catcgtgacc ccatgcatcg agaagagttg gtcgtaggcc tccggcgaca gcacctgcaa
     5281 gcccggacgg gtgagctgga cccgcaggag catcgccccc accccggcga tggcgaagaa
     5341 gacgaagccc gtgtacaggt agcgcatccc gatgcgcttg tgatcgacgg tcccgatcca
     5401 gctggccagt cccggggtct cgccccagac ctcgtccagg cgctcggcga tcgacgccat
     5461 cagttcagcc ccatcaggta gtcgaccaac gactgcagct gtgaccgggt cagctgggtg
     5521 ggcggcatga tcacccccgg tttgacggcc tgcggatccc tgatccagtg cgcgaggttc
     5581 ccgcgcgtga gccggagcgt tcccgccgcg atcgtctccc ggtccgccag atgggtgagg
     5641 tcgggcgcga gcgcggccga ggcgggggtg ccgcggatcg tgtggcagcc gtcgcaggtc
     5701 tgggccagga agacggccag gccgtcggcc gccgacccgg tgggcgcggc ggccggcgcc
     5761 tcctggtggg ccatccaccg ctggaagaca tcaggcgggt ccgccaccac gaagaaggcc
     5821 atgtgcgcgt gctgaagacc gcagaactcg gcacactgtc ctcggtaccg ccccgggcta
     5881 tcggctacca gcacgatcga gttcgtcttg ccggggatca tgtcgatctt cggcgccagc
     5941 tggggcaccc agaagctgtg gatgacgtcg gcggtggtca cctcgatccg gacgggctca
     6001 ccggccggga tatggatctc gttcgcggtg atggcgccgt tcgggtagcg cgcctcccac
     6061 caccacatgt ggccgatcac ctggacggtc agctgtggat cgccgccggc gatcgcgttc
     6121 atgtcgcgga cggagaccac gaagaccgcg gtgaggatca gcaccgggat gatcaccccg
     6181 gccagcacca cgaacggctc cccccagcgg ggctcggatc ggtccacgtc cgtgcctcgt
     6241 cttcgacggc ggatgatggc cgccgtcatg agcccgagga ccaccgccag caccacggcg
     6301 gagatggcga agaggagcca ccacagcccc tccacccgct tctcgctggc cccgtgcgcg
     6361 tcgagcacgg acggcccccc gagcgcacag gcgctcgtgg cgagcgcgat cacgatccag
     6421 ctcgcgcccg ctcggatccc gcgtgacggg atccgtcgac ccttcccccc acatgagctg
     6481 ctacgcaaaa cgatccagtt gttccccccc gccgcggttc tcaaccaccc tgcccggagc
     6541 cccccgatcg cgcctcccgc aacaggcctt gcgcctcctg ccggtgc
//
LOCUS       DASQQQ010000018        26125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_83574, whole genome shotgun sequence.
ACCESSION   DASQQQ010000018 DASQQQ010000000
VERSION     DASQQQ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 26125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26125
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83574"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1361
                     /locus_tag="VE646_00780"
     CDS             <1..1361
                     /locus_tag="VE646_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HZD78564.1"
                     /translation="SRDLLEDEPQNVVARLRLREGDRALIDAMQDPAFGELLLDSVRR
                     RRRFPGGKGRVETSMTKVFRSLRGPVSEPLEARPMQAEQSNSSVIFGDRLVLKLFRRT
                     EEGPNPDLEIGRFLTERARFANIPPVAGGLEYRHGRGPAQTLGIVHGFVPNEGDAWGF
                     TIDAVAGYLERAQARPTEDLEGVFVPATPLEAVDVEPSPLAEETIGAYLEAAWRLGAR
                     TAELHLALSSDQEDPAFRPEAATGHYQRSLYQSMRGTANRVFQLLRAHRGDLPDAERV
                     LELQPEILARLHAVLEPKLTAYRTRIHGDYHLGQVLYTGKDFVIIDFEGEPARPIGER
                     RLKRLVVRDLGGMLRSFHYAVSMPVLGRPGLPAPEETAHLRPWMRLWYVTIGGLFLRS
                     YLETADQAAFMPRSREELAVLLDASVLEKALYELAYEANNRPEWLPIPIGGILDLLGV
                     AD"
     gene            1307..3439
                     /gene="malQ"
                     /locus_tag="VE646_00785"
     CDS             1307..3439
                     /gene="malQ"
                     /locus_tag="VE646_00785"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HZD78565.1"
                     /translation="MAADPDRRHPRPAGGRGLRPAPDREGSSLAELARLHGIQGSYVD
                     LRGQRRRASREATLAVLRALGAPVDRASDIPEAIRERREEGWGQLLEPVAVVWEGLTA
                     AFDLRMPPGVEPTFVELRLELEGGGSVRGWKERVEDLVPLPPEPEASGLGVRRRVVLS
                     DPLPLGYHRLWASAGGRQASCLVISAPRRTDPSPDPGTWGVFLPLYALWSKRSWGVGD
                     LTDLGALRDWVVGLGGRLVGTLPMFAAFLRDPGEPSPYSPISRLFWNELFVDVEAAPE
                     LAANAEARRLISAPGTRRAIGRLRRRPLVDHAQAAALKRSVLELLTDRLHRSGSRRRD
                     ALEEFAREHPALDDYARFRAAGERWGVNWRVWPGPARDGRLETANVDSAATRFHRYAQ
                     FLADEQMGELRRVSEEGGAGLYLDMPLGVHPDGYDVWRERESFAQGVSAGAPPDDFFQ
                     GGQDWGFPPPHPDRIREEGYRYQIACLRRAMAHAAALRIDHVMGLHRLYWVPEGASAE
                     QGLYVRYRPEEWYAIVALESRRSGTAIVGEDLGTVPPAVRASMDRHGLLRSYVLQMEA
                     GPDGLRPPPARSLATLNTHDMPPFAAWWDGTGGRTRSAVARDLRRLGFLVRGAERDEA
                     AVLRACLAYLGRSPARIAIANLEDLWGEHEPQNRPGSTGYPSWRRRAAHPLEELPRLP
                     EVADTLAEVGVLRRQRGAPSRRGAGGIT"
     gene            3436..5343
                     /gene="glgB"
                     /locus_tag="VE646_00790"
     CDS             3436..5343
                     /gene="glgB"
                     /locus_tag="VE646_00790"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HZD78566.1"
                     /translation="MSQRVRHDVSLLSDDDVFLFNEGTHARLYRRLGAHLLSHEGVEG
                     TYFAVWAPDAESVSVIGDFNGWDRGADRLQPHASSGIWEGFLAGVGEGAVYKFHVASR
                     FAGYRVDKADPFAFLAETPPKTASVVWPLDFEWGDQAWMRERHRANALDAPLAIYEVH
                     LGSWMRGEGDRILSYRELAPRLAEHAKALGFTHVELLPVMEHPFYGSWGYQTTGYFAP
                     TSRYGTPQDLMAFMDHLHREGIGVILDWVPSHFATDEHGLGRFDGTHLYEHADPQLGF
                     HPDWGSYVFNYGRNEVRSFLLSSALHWLDTYHADGLRVDAVASMLYLDYSRGPGEWIP
                     NRHGGRENLEAIDFLRQLNVAVYRDHPGVQTYAEESTAWPRVSRPVEHGGLGFGLKWD
                     MGWMHDTLEYFDRQPVHRSYHHDRITFRMLYAFNENFVLPLSHDEVVHGKGSLLSKMP
                     GDEWQRFANLRLLLSYMYAQAGKKLLFMGSEFGQGSEWRHDHSLDWHLLEHEPHLGVQ
                     RLVADLGRIYREQPALHELDCDPAGFEWVDASDAAGSIMSFLRKSSSGAPPVLVVCNF
                     TPVVRHGYRIGVPSTGRWTELLNSDAVGYGGSGQGNLGGVEAEEEPMHGRAWSVPLTL
                     PPLACLFLTGS"
     gene            5352..7196
                     /gene="treZ"
                     /locus_tag="VE646_00795"
     CDS             5352..7196
                     /gene="treZ"
                     /locus_tag="VE646_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HZD78567.1"
                     /translation="MDPFSAPLGATPMPEGGCAFVVWAPLAERVEVLVGPPAGRTIAL
                     EPAERGYHAALVAEVGPGDRYLLSLDEAEGVPDPASRSQPEGVHGASEVVDRTAFAWT
                     DEGWSAPPLGEQVLYELHVGTFSEAGTFEGVRERLDALVQLGVTGVELMPVAQFPGER
                     NWGYDGVFPFAAQDSYGGPAGLARLVDACHGAGLSVFLDVVYNHVGPEGNVLPRFGPY
                     FTDRYRTPWGDAVNLDGPGSDEVRRFFIDDALGWLEEFHLDGLRLDAIHAIVDQTARP
                     FLAELGAEVATLSERLGRRLFVIGETPENDARVVTPRELGGMGLDALWSEGFHHALHA
                     LVTGERDGYYVDYGTLELMARAMTEGWVFAGQYSRFWGRRHGSSPRPVTPDRFVVYAQ
                     NHDQVGNRALGERLSALVPFEDLKLVAGLLLLSPFVPLLFMGEEYGELAPFRYFVSHG
                     DPDLIEAVRRGRREEFAAFGWPGEPPDPQDLRTFLRSRLRPGLREREPHRFLLELYRE
                     LLRLRRERPALGRSTKEGMEIWADSGTGVLQLRRRTEEDEAVAYFHFGSEPATVTLPE
                     GDWTLAVDSSDPRWGGPEATAPTALRGPAERSIVHPRSFVLFVSRGEG"
     gene            7198..9321
                     /gene="glgX"
                     /locus_tag="VE646_00800"
     CDS             7198..9321
                     /gene="glgX"
                     /locus_tag="VE646_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HZD78568.1"
                     /translation="MRVWPGTPTPIGATWDGQGVNFALFSEHAESVDLCLFDAPEQEH
                     ESQRIRLRERTDFIWHAYLPDVRPGQFYAYRLRGPYEPEAGHRFNPSKLLLDPYAKAV
                     SGSLRWDDALYGYTIGHQEEDLSRDDRDSAPFLPKCMVVDPAFTWGLDRPPRTPWSRT
                     VIYECHVRGMTMLHPAVADELRGTYLGLASDPVIDHLLSLGVTAVELLPVHQSVADRH
                     LVERGLTNYWGYNSIGFFAPGARFATGDRGQQVAEFQSMVRAFHEAGLEVILDVVYNH
                     TAEGNQLGPTLSFRGIDNAAYYRLIPDNGRYYMDFTGTGNSLNVLHPRTIQMIADSLR
                     YWVQEMHVDGFRFDLAPVLGREPLMVDPGGTFFDVVMQDPVLSEVKLIAEPWDLGHDG
                     YQVGRFPPGWAEWNGRYRDTIRRFWKGEPGQVPELASRLSGSGDIYQGSGRRTYASVN
                     FITAHDGFTLNDLVSYEQKHNEQNGESNRDGANDNYSRNWGVEGPTESTRITSMRERM
                     KRNFLATLLFSQGVTMLLGGDEIGRTQYGNNNAYCQDNEISWPSWNLSQRDRELLRFT
                     RSVLRIFRSNPVLRRRSFLTGQPVGGNGTKDLTWVRPDGHEMQGEDWNDPGTHILGML
                     LSGHATDEVDQRGRPVVGSTVLLLLNGGARSRRFVLPKVEGPGLWLELINTARPGSRV
                     VKTGTVNLVAHSLLLLRFGEERTRR"
     gene            9318..11927
                     /gene="treY"
                     /locus_tag="VE646_00805"
     CDS             9318..11927
                     /gene="treY"
                     /locus_tag="VE646_00805"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HZD78569.1"
                     /translation="MSARPAGAPRRPVGTYRLQLRQGVGFADARRLLPYLSRLGVSDL
                     YLSPPFAAQPGSAHGYDVIDPNRLDPALGGRRAFEALARELRSSGLGLLVDIVPNHLA
                     ATPANPWWRDVLRRGRGSPFARFFDIDWDAPGADGKLLLPVLERPLEELLAEGAVGLE
                     EEGREVVVALAGLHLPISPASERAVRERGLGAWNRGRASPQSARTLRELIEEQAYRPL
                     WWREGDRRVNYRRFFDIAGLAGLREEDPRVFDRVHAAVVRLVQEGTITGLRIDHVDGL
                     LDPEDYLLRLRRATRVPYLAVEKVLHRDEVLPGSWPVQGSTGYALLNDLTDLTVDPQG
                     WRELGAVTERLRGDPAPPFERIRLEARRQVLRELFGSEVQALVRRVARLASGAALDLE
                     EGALREAVVELTAALEGYRTYVGPEGAGPEDRRRIERAVRAARGHLPRSARPALRFVH
                     RVLTLELPPVATARTRSAWLEVVERWQQLSGPAAAKGVEDTALYREVRLICRNEVGAD
                     PGREPADLPGFHRRIRELHEGPHPLIATSTHDTKRSEDVRARIAVLSELPGEWERRAA
                     RWIRANRRHRRGVHGHDAPDRDEELLLYQTLLGAWPQDEDLGPFAERIRAYVVKAARE
                     AKVHTSWLDPDAAHERTLEAFATAVIADQRFLRDFLPLQRTVAFHGALNSLSQVVLRT
                     ALPGVADLYNGTETWDLSLVDPDNRRPLDIRALARRLAELDRRQPREGADLASDLIGA
                     WPDGLVKLFAVSRCLRLRRERPDVFVEGSYLPLQVHGPAAAHAVAIARRAGERWALAI
                     VPRLTLRLAGPGRAPIGERIWAETVAMLPRGAPRTWVDAFTARSVGPDARGALALGQV
                     LAPFPVALLVPAE"
     gene            complement(11940..12575)
                     /locus_tag="VE646_00810"
     CDS             complement(11940..12575)
                     /locus_tag="VE646_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZD78570.1"
                     /translation="MVAPPSSLRERILDAAQRLVLERGFTATTVDAVLETAGASKGAF
                     FHHFPTKASLGRALVERYAAGDAEVLETLMQKAEADSDDPAEQVIGFVRAFEGTAEEL
                     ALSQPGCLFVSFIYEHMPSSQDVNDIIVGSIRLWRERILEKLRQAAQAHPPAVHVDLP
                     SLADQVSTIFEGAFILARATEDPGYLRTQLSHLRHYLELLFEADAPHARHR"
     gene            12666..13808
                     /locus_tag="VE646_00815"
     CDS             12666..13808
                     /locus_tag="VE646_00815"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019050164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZD78571.1"
                     /translation="MASDTTTVEAVPASEAGTDGSAETTFPERVLGSILGTIDVFTMY
                     LGEKLGLYRALGDHGPLTPGGLSERAGVHPRYAREWLEQQTVSGILAVDDPRREPSER
                     RYSLPASQAEVLLDRDSLDYLAPFVRVVMAGGLAMPALIEAYRSGGGVSWGQLGEDAR
                     SGQAEMNRPWYLRSIGTEWFPKIPVLHERLAAGARIADIACGEGWSSIAIALAYPATT
                     VDGYDVDEPSIQAARRHAEEAGVADRARFRVADVAAVDLEGVYDAVVGFEFVHDLPHP
                     VEVLRAMRRLVKPDGPVVVLDENVPDRFTGEPNDVERVMYGFSVLVCLPDGMSHRPTV
                     ATGTVIRADTMRAYAQQAGFADVEIEPIDHDLWRFYRLVHEGIQMG"
     gene            complement(13830..15023)
                     /gene="glgA"
                     /locus_tag="VE646_00820"
     CDS             complement(13830..15023)
                     /gene="glgA"
                     /locus_tag="VE646_00820"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HZD78572.1"
                     /translation="MKVVLMTREYPPEVYGGAGVHVEYLARDLADLIEVEVRCFGADR
                     DPGAHPPVRAFRPWQQLSGAPSVLGVLSVDLAMAADLGGASLVHSHTWYANIAGHVAK
                     LLHGVPHVATTHSLEPLRPWKSEQLGEGGYELTRFIERTALEHADAVVAVSEGMRADI
                     LRVYSDLDPARVRVIHNGIDAEEYAPDDGTEVLAAHGIDPDRPSVIFVGRITHQKGVT
                     YLLDAARQIDPQAQLVLCAGAPDTPEIGAECEAKVDRLRTERGNVIWIREMLPKPSVI
                     QLLTHATVFVCPSIYEPLGIVNLEAMACETAVVATAVGGIPEVVVDGETGILVPFEPG
                     DQSRPKDPEGFARGLAEGVNELLADPGRAEVMGKAGRQRVLEQFTWTEVARRTLGLYE
                     ELAGG"
     gene            complement(15067..16731)
                     /locus_tag="VE646_00825"
     CDS             complement(15067..16731)
                     /locus_tag="VE646_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZD78573.1"
                     /translation="MHVARALEQGDVHTIFALPGGHITPLYDGARHTGVRIVGARHEE
                     NAVFMAEAWALATAQVGVAAVTAGPGLANALPGMAEANAAGVPVVVIAGRTGIEQRGR
                     GAVQDMDQLAAVAPLTKWRAECLKPERVGWFVSEALHQARAGAPGVAYLEVPQDVLAA
                     TTEPYQAPWPVGHEAEPARSVPAPSDLRRAMDLLSEAERPVALAGSGAFFSGAGQALR
                     NFAERTGVPVTTTSAARGLLDDDHPRCLGGLVHGGIALASADVALILGSRFNANVTYG
                     GAPLFQPDQRVVQVDVRPEHLGGWRRPQVALAGDVATTLEALTDAWQPPAEALDGWME
                     QCREGVASSRQLWQMEGDRPANGIHPGWLAREVARFAREQGPHTFVSDGGDSVLWGIA
                     LSTAHAPGTNLFIGSAQGTLGIGLPFGIGAKVARPAEPVFVFTGDGAFGLSAIELDTA
                     ARNGAGVVVVVVDNGGWGDVRHEQRMFYGEESVLHSDVSSMRYDLLAQAVGGHGERVE
                     AAEGLRPALERATEASAKGVPAVVDVATDPDAMSDLMSDLSGLAVM"
     gene            16870..17148
                     /locus_tag="VE646_00830"
     CDS             16870..17148
                     /locus_tag="VE646_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020022882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78574.1"
                     /translation="MTEMQITELEPDRYAVELTEGNEHTSHVVEVPEDMLDDLGLVDA
                     DHEEVVRESFAFLLEREPATSILSGFSLEQIANYFPEYYDELKLRLSP"
     gene            complement(17176..17763)
                     /locus_tag="VE646_00835"
     CDS             complement(17176..17763)
                     /locus_tag="VE646_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011057625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZD78575.1"
                     /translation="MARIWSMLELGARAPDFELPDVTTGRLVRRSDLDDHDALLVLFL
                     CRHCPYVVHVQDGIAGLTHHYRGSGLAIVGISANDPDAYPEDAPESLAEQAAQVGFDF
                     PYLFDQRQEVAMAYTAACTPDPFLFDWDRRLVYRGQLDGSRPGNGVPVTGEDLRSAID
                     AVLADRPVPEDQRPSVGCSIKWRPGNEPRPIFTLR"
     gene            complement(17805..18554)
                     /locus_tag="VE646_00840"
     CDS             complement(17805..18554)
                     /locus_tag="VE646_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZD78576.1"
                     /translation="MSTGRRLALGALALGGAYWGPGLAAVPSVGRRLGRWVLTAVSTE
                     EPAVALTFDDGPDPRFAERFLEALGQARCTFFWLGSSVRRWPDLARAARAAGHEIACH
                     GDTHHSMGRAGPVGTARSLRAARDAIGSATGRAPAFYRPPYGWFTAPAWTVCERLGLR
                     RTLWTASAGDWRSDRSSAQIAQATLAAVRPGAILLLHDGRGQNGAPLRTLAAVPAILQ
                     GLSTRGLRPVTLSELVRLGMERPAAQPGMSG"
     gene            complement(18551..19471)
                     /locus_tag="VE646_00845"
     CDS             complement(18551..19471)
                     /locus_tag="VE646_00845"
                     /inference="COORDINATES: protein motif:HMM:NF038012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZD78577.1"
                     /translation="MATSLGAYAITIGFATLAGAALAVNGYLEQREASSKPEQGRFKI
                     RLMEQLVTDRVWLLAMAFGFLSYVFQAVALAFGPLSMVQPLVLSELIFAVPFSIRRHH
                     RRMGVREWVGILSVAGGLAVGLVAASPRRGNPLPDIVPWAITIGVVAVLAAAAVLGGR
                     RLRGPAQASILAIAAASVLGLQSAFLDSATSLADARFVELFTHWQGYAIVPATVIGIW
                     LMQVAYQAGPLAASMPVVDAFQPSVAIAIGVVLFGEPIATGTWNLVGTGFGLAMFFCG
                     IVLLDTSPLVRKIQRLEQEEREGADRESAA"
     gene            complement(19479..20678)
                     /locus_tag="VE646_00850"
     CDS             complement(19479..20678)
                     /locus_tag="VE646_00850"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HZD78578.1"
                     /translation="METPRPGLGGSRSGRRALILTASMGSGHVSVGLELAARLRSRGY
                     RTRLADVHDLMPRPAGQWIAGFYFWTLRRAPWVYQAIFQAFFKHPQERGERVRIPVQL
                     ALPGLRRLVASFEPDVVVSTYHIAGLAAAELRGRGALGCPVISFVPTFGVHELWMHPD
                     IDGYLCISASVADYLASRTQRPTRTCGPLVRSAFLSPDGRSGHEPLPVEIPQAVRSAL
                     VVGGSLGIGDMLRAARGVTSMPGWMAVAVCGRNEQLRGQMERVPGCTALGWVEDMAGL
                     MRVCDVLIDNAGGLSSKEALAAGLPVVTFRPIPGHGRDDALQMQRLGLTEVVEDEGDL
                     VAALTRLAGSPAERRRRSRRGRSLFVADAAAELEELIRITERPRVAVPHAIRRGPRPL
                     PSRRLTG"
     gene            complement(20756..21634)
                     /locus_tag="VE646_00855"
     CDS             complement(20756..21634)
                     /locus_tag="VE646_00855"
                     /inference="COORDINATES: protein motif:HMM:NF038012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZD78579.1"
                     /translation="MVFALSLLAALSFALAAVLHQRAAARAPEHAGTRVPVLTWLFRR
                     PIWLGGTFFMLFGYALQAVALSFGSLVTVEGLLTTNLVFALPIASAWGGRRMRPIEWA
                     AMVATTGGLVLFLVMRGPPAAGGDPSATRWLYTFGAAAVLVAAAHAITLRSPPAIRAL
                     VLGAAAGVAFGLADGLTKALVPIFEQRRLGGFLSWQLYLVGIWGGGGLIVSQAAYHVA
                     ALRDSFPAMNVAEPLAGAALGVLLFSESLPTGAPAIALDAVAIVIMLAGVVALARSDL
                     IVGAPHGPRSDPSGSR"
     gene            complement(21706..21942)
                     /locus_tag="VE646_00860"
     CDS             complement(21706..21942)
                     /locus_tag="VE646_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78580.1"
                     /translation="MVTAQELAEKAMELAEGCLSGRADELYEVAGEDPDLVTRASQIV
                     RAHRSRAPETDRQVEHLAFILLTSVFTRMREETA"
     gene            complement(22210..22557)
                     /locus_tag="VE646_00865"
     CDS             complement(22210..22557)
                     /locus_tag="VE646_00865"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZD78581.1"
                     /translation="MVEGNTGRLVKTTGDGILATFDGPGRAIRAAAALRDELAIVDIS
                     IRAGIHTGEVELRDGDVGGIAIHLAARIMSVAEPAEILVSNTVRDLVIGSDLAFRDRG
                     THTLKGIDDRWQL"
     gene            complement(24079..24152)
                     /locus_tag="VE646_00870"
     tRNA            complement(24079..24152)
                     /locus_tag="VE646_00870"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24116..24118),aa:Lys,seq:ctt)
     gene            complement(24186..24262)
                     /locus_tag="VE646_00875"
     tRNA            complement(24186..24262)
                     /locus_tag="VE646_00875"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24226..24228),aa:His,seq:gtg)
     gene            24537..24893
                     /locus_tag="VE646_00880"
     CDS             24537..24893
                     /locus_tag="VE646_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78582.1"
                     /translation="MVLPGAAYAKGPPATSFCVDGTLYWTVGTPTDLSNTGAPDSAFD
                     TIYEFFGAQPDNVATAAPGDRDHNGGRWHVHGLSFPDGYAAALSSGDRTTTASSTTTP
                     RCRRRFGAGRRWTWGS"
     gene            complement(24965..26122)
                     /locus_tag="VE646_00885"
     CDS             complement(24965..26122)
                     /locus_tag="VE646_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023524285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HZD78583.1"
                     /translation="MNERQRRLLLAAEARELGRGGVAAVARAASVSRPTVRKAIRELE
                     EEPAPTSGRSRRPGGGRKQLAEKDPALLQALEELVDPSTRGDPMSPLRWTCKSTRQLA
                     QALTETGHPVSSWTVAKLLHWAGYSLQANRKTMEGTHHPDRDAQFRYVNERVRAHLRR
                     GDPVISVDTKKKELVGSYKNGGREWQPKGEPEEVNIYDFISPAVGKAIPYGIYDVGAN
                     TGWVSVGSDHDTASFAVEAVRRWWNGVGRDAYPRSTRLLICADPGGSNGYRVRLWKLE
                     LSRLATETGLSVTVCHLPPGTSKWNKIEHRLFAHISMNWRGRPLTSHEVVVELIGATT
                     TATGLAVHAERDAGAYPLQVKVSDEQFAAIPLVPHRFHGEWNYTIRPGRTG"
BASE COUNT         3673 a         9340 c         9416 g         3696 t
ORIGIN      
        1 actcgcgcga cctgctggag gacgagcctc agaacgtggt ggcccggctg cgcctgcgcg
       61 agggggaccg ggcgctgatc gacgcgatgc aggatcccgc gttcggcgag ctgctgctgg
      121 attcggtccg ccgccgccgc cggttccccg gcggcaaggg gcgggtggag accagcatga
      181 ccaaggtctt ccgctcgctc cgcggaccag tgtccgagcc gttggaggct cggccgatgc
      241 aggcagagca gagcaactcc tcggtgatct tcggcgaccg cctggtgctc aagctcttcc
      301 gccgcacgga ggagggcccg aacccggacc tggagatcgg gcggttcctg accgagcggg
      361 cccggttcgc gaacatcccg ccggtggccg ggggcctcga gtaccggcac gggcgcggtc
      421 ccgcacagac gctcgggatc gtccacggct tcgtgcccaa cgagggcgac gcctggggct
      481 tcacgatcga tgccgtagcc ggctacctgg agcgggccca ggcccgtccc accgaggacc
      541 tggagggggt cttcgtgccc gccacccccc tggaggccgt ggatgtggag ccctcgccgc
      601 tggccgaaga gacgatcggc gcgtacctgg aggcggcctg gaggctcggc gcccgcaccg
      661 ccgagctgca cctggccctg tcctcggacc aggaggatcc ggccttccgg cccgaggcgg
      721 cgaccggcca ctaccagcgc tcgctgtacc agtcgatgcg cggcacggcc aaccgcgtgt
      781 tccagctcct gcgggcgcac cgaggggacc tgcccgacgc cgagcgggtc ctggagctgc
      841 agcccgagat cctggcccgg ctgcacgccg tgctggagcc caagctcacc gcgtaccgga
      901 cccggatcca cggcgactac cacctcggcc aggtgctcta cacgggcaag gacttcgtga
      961 tcatcgattt cgagggggag cccgcgcggc ccatcggcga gcgacggttg aagcggctgg
     1021 tggtgcggga cctgggcggg atgctccgct cgttccacta cgcggtgagc atgccggtgc
     1081 tggggcggcc cggcctgccg gctcccgagg agacggcgca cctgcggccc tggatgcgcc
     1141 tgtggtacgt cacgatcggg ggcctgttcc tccgctcgta cctcgagacc gcggaccagg
     1201 cggcgttcat gccgcgctcc cgcgaggagc tggcggtgct gctggacgcc tcggtgctag
     1261 agaaggccct gtacgagctg gcctacgagg cgaacaaccg gcctgagtgg ctgccgatcc
     1321 cgatcggcgg catcctcgac ctgctggggg tcgcggactg aggccggccc ccgaccgcga
     1381 gggctcctcg ctcgccgagc tggcccgtct gcacggcatc cagggcagct acgtggacct
     1441 gcgggggcag cggcgacggg cctcgcggga ggccaccctg gccgtcctgc gcgccctcgg
     1501 ggccccggtg gaccgcgcct cggacatccc cgaggcgatc cgggagcgcc gcgaggaggg
     1561 ctgggggcag ctgctcgaac cggttgcggt ggtgtgggag ggcctcacgg ccgccttcga
     1621 cctgcggatg ccgccgggtg tcgagcccac cttcgtggag ctccgcctgg agttggaggg
     1681 cgggggcagc gtgcggggat ggaaggagcg ggtggaggac ctggtgcccc tgccgccgga
     1741 gcccgaggcc tcggggctgg gcgtccgccg gcgggtggtc ctgtccgacc ccctgcccct
     1801 cggctatcac cggctgtggg cgtccgccgg gggacgccag gcctcctgcc tggtgatctc
     1861 ggcgccgcgg cggaccgacc ccagccccga tccggggacc tggggggtgt tcctcccgct
     1921 gtacgcgctg tggtccaagc ggagctgggg cgtgggcgac ctgaccgatc tcggagcgct
     1981 ccgggactgg gtggtgggcc tgggcggccg gctggtgggg accctgccga tgttcgcggc
     2041 cttcctgcgc gacccggggg agccgagtcc gtactccccg atcagccgcc tgttctggaa
     2101 cgagctgttc gtcgacgtgg aggcggcgcc ggagctggcc gcgaacgccg aggcccgccg
     2161 gctgatctcg gcgccaggca cgcggcgggc catcgggcgc ctgcggcgcc ggccgctggt
     2221 ggaccacgcc caggccgcgg cgctcaagcg gagcgtgctg gagctcctga ccgaccggct
     2281 ccaccggtcg ggctcgcgcc gccgcgacgc cctggaggag ttcgcgcggg agcaccccgc
     2341 cctcgacgac tacgcgaggt tccgggccgc cggcgagcgc tggggggtga actggcgcgt
     2401 gtggcccggt ccggctcggg acgggcgact ggagacggcc aacgtggata gcgccgccac
     2461 ccgcttccac cgctacgcgc agttcctggc cgacgagcag atgggggagc ttcggcgggt
     2521 ctcggaggag ggcggtgccg gattgtacct ggacatgccg ctcggcgtgc accccgacgg
     2581 gtacgacgtg tggcgcgaac gcgagagctt cgcccagggc gtctcggccg gggccccgcc
     2641 ggacgacttc ttccagggcg gccaggactg ggggttcccg ccgccgcatc cggaccggat
     2701 ccgggaggag gggtaccggt accagatcgc atgcctgcgc cgggccatgg cccacgcggc
     2761 ggcgcttcgg atcgaccatg tgatgggact gcatcgcctg tactgggtgc cggagggcgc
     2821 ctcggcggag caggggctgt acgtccggta ccggcccgag gagtggtacg cgatcgtggc
     2881 cctcgagtcc cggcgctcgg gcaccgcgat cgtgggcgag gacctcggca ccgtgcctcc
     2941 cgcggtgcgc gcctcgatgg accggcacgg gttgctccgc agctacgtgc tgcagatgga
     3001 ggccggtccg gacggcctgc ggccgccccc ggcgaggtcc ctggccaccc tgaacaccca
     3061 cgacatgccg cccttcgcgg cctggtggga cgggacgggc ggtcgcacgc ggtccgcggt
     3121 ggcgcgggac ctgcggcggc tgggcttctt ggtccggggg gccgagcgcg acgaggccgc
     3181 ggtgctccgc gcctgcctcg cctacctggg ccgctcgccg gcccggatcg cgatcgcgaa
     3241 cctggaggac ctgtggggtg agcacgagcc gcagaaccgt cccgggtcca ccgggtaccc
     3301 gagctggcgc cgccgggccg cccatccgct cgaggagctg cctcggctcc cggaggttgc
     3361 cgatacactc gcggaggtgg gggtccttcg acgacagagg ggcgcgcctt cccgccgcgg
     3421 ggccggaggg atcacgtgag ccagcgcgtg cggcacgacg tctccctgct ctcggacgac
     3481 gatgtgttcc tgttcaacga gggcacgcac gcccgcctgt accgcaggct cggcgctcac
     3541 ctgctcagcc acgagggcgt ggaggggacg tacttcgcgg tctgggctcc cgacgccgag
     3601 tcggtctcgg tgatcggcga cttcaacggc tgggaccggg gggcggaccg gctgcagccc
     3661 cacgcgagct cgggcatctg ggaggggttc ctcgccgggg tgggcgaggg cgcggtctac
     3721 aagttccacg tggcctcccg cttcgcgggc taccgcgtgg acaaggccga cccgttcgcg
     3781 ttcctggccg agacgcctcc gaagacggcc tcggtggtct ggcccctgga cttcgagtgg
     3841 ggggaccagg cctggatgcg cgagcgccac cgggcgaacg ccctggacgc gccgctggcg
     3901 atctacgagg tgcacctcgg ctcgtggatg cggggtgagg gcgatcggat cctcagctac
     3961 cgagagttgg ccccgcgcct ggccgagcac gcgaaggccc tgggcttcac gcacgtggag
     4021 ctcctgccgg tgatggagca tcccttctac ggctcgtggg gctaccagac cacgggctac
     4081 ttcgcgccca ccagccggta cgggacgccc caggacctca tggcgttcat ggaccacctg
     4141 caccgcgagg ggatcggcgt gatcctggat tgggtgccct cgcacttcgc caccgacgag
     4201 cacggcctgg gccggttcga cgggacccac ctgtacgagc acgccgaccc ccagctcggc
     4261 ttccacccgg actggggcag ctacgtcttc aactacggcc gcaacgaggt gcggagcttc
     4321 ctgctctcca gcgccctgca ctggctcgac acctaccacg ccgacgggct tcgggtcgac
     4381 gccgtggcct cgatgctcta cctggactac tcgcgggggc cgggggagtg gatcccgaac
     4441 cgtcacggcg gccgggagaa cctcgaggcg atcgacttcc tgcgccagct gaacgtcgcc
     4501 gtgtaccgcg atcacccggg ggtccagacc tacgccgagg agtccacggc ctggcccagg
     4561 gtgtcccggc ccgtagagca cgggggtttg ggcttcgggc tgaagtggga catgggatgg
     4621 atgcacgaca cgctcgagta cttcgaccgc cagccggtcc atcgcagcta ccaccacgac
     4681 cggatcacgt tccggatgct gtacgcgttc aacgagaact tcgtgctgcc cctctcgcac
     4741 gacgaggtgg tgcacggcaa gggctcgctg ctgtcgaaga tgccgggcga cgagtggcag
     4801 cggttcgcga acctgcggct gctcctgtcc tacatgtacg cgcaggccgg caagaagctg
     4861 ctgttcatgg gctccgagtt cgggcagggc tcggagtggc gccacgatca ttcgttggac
     4921 tggcacctgc tagagcacga gcctcacctg ggggtccagc ggctggtggc ggacctgggc
     4981 cggatctatc gggagcagcc cgccctgcac gagctcgatt gcgacccggc agggttcgag
     5041 tgggtggatg cctcggacgc cgcgggcagc atcatgagct tcctgcgcaa gtcctcctcg
     5101 ggcgcaccgc cggtgctggt ggtctgcaac ttcaccccgg tggtccgcca cggctaccgg
     5161 atcggcgtgc cctcgaccgg tcggtggacc gagctgctga acagcgatgc cgtgggctac
     5221 ggcggcagcg gccagggcaa cctgggcggg gtggaggcgg aggaggagcc gatgcacggg
     5281 cgggcgtggt cggtgcccct cacccttccc cccctggcct gcctgttcct gaccgggtcc
     5341 tgagcgcccc cgtggacccg ttctcggccc cgctcggcgc cacccccatg ccggagggcg
     5401 gctgcgcctt cgtggtgtgg gcgccgctcg ccgagcgggt ggaggtcctg gtgggtccgc
     5461 cggcgggccg gacgatcgcc ctggagccgg cggagcgcgg gtatcacgcc gccctggtgg
     5521 cggaggtcgg gcccggggac cgctacctgc tgtcgctgga tgaggccgag ggggtcccag
     5581 acccggcctc acgcagccag cccgagggcg tgcacggggc ctcggaggtg gtggaccgca
     5641 ccgccttcgc ctggaccgac gaggggtggt cggccccgcc cctgggcgag caggtgctgt
     5701 acgagctgca cgtgggcacg ttttccgagg ccgggacctt cgagggggtc cgggagcggt
     5761 tggatgcgtt ggtccagctc ggcgttacgg gggtggagct gatgccggtg gcgcagttcc
     5821 ccggcgagcg gaactgggga tacgacggcg tgttcccctt cgcggcccag gacagctacg
     5881 ggggaccggc cgggctggct cgcctggtag acgcctgtca cggcgccggc ctgtcggtgt
     5941 tcctggacgt ggtgtacaac catgtgggac ccgaggggaa cgtcctgccg cggttcggcc
     6001 cgtacttcac cgaccgctac cgcacgccct ggggtgatgc ggtgaacctg gacgggccgg
     6061 gcagcgacga ggttcgtcgg ttcttcatcg acgacgcgct ggggtggctc gaggagttcc
     6121 acctggacgg cctgcggctg gacgcgatcc acgcgatcgt ggaccagacc gcccgcccct
     6181 tcctggccga gctgggggcc gaggtggcga cattgtccga gcgtctgggg cgccggctgt
     6241 tcgtgatcgg cgagacgccc gagaacgacg cgcgcgtggt gaccccccgc gagctcggcg
     6301 ggatgggcct ggacgccctg tggagcgagg gcttccatca cgccctgcac gccctggtca
     6361 ccggggagcg ggacggctac tacgtcgatt acggaacgct ggagctgatg gcccgagcca
     6421 tgaccgaggg ctgggtgttc gcgggacagt actcgcggtt ctgggggcgg cggcacggca
     6481 gctcgccgcg cccggtgacc cccgaccgct tcgtggtcta cgcccagaac cacgatcagg
     6541 tgggcaaccg ggcgctgggg gagcgcctgt cggcgctggt gcccttcgag gacctgaagc
     6601 tggtggccgg gctgctcctg ctctcgccgt tcgtgccgtt gctgttcatg ggcgaggaat
     6661 acggcgagct cgccccgttc cggtacttcg tgagccacgg cgatcccgac ctgatcgagg
     6721 ccgtgcgccg cgggcgtcgg gaggaattcg ccgccttcgg ctggccgggt gagccgcccg
     6781 acccccagga cctgcgcacc ttcctgcgct cccggctccg gcccgggctc cgcgagcgcg
     6841 agccccaccg gttcctgctg gagctgtacc gcgagctgct gcgcctgcga cgagagcgcc
     6901 cggcactggg gcgctccacg aaggagggta tggagatctg ggccgactcg ggcaccgggg
     6961 tcctgcagct ccgtcgccgc accgaggagg acgaggccgt cgcctacttc cacttcggga
     7021 gcgagccggc cacggtgacg ttacccgagg gcgactggac cctggccgta gactcctcgg
     7081 acccgcggtg gggtggaccg gaggccacgg cgccgaccgc gttgcgtggt ccggccgaac
     7141 ggtccatcgt gcatccccgg agcttcgtgt tgttcgtgag ccggggggag ggctgaggtg
     7201 cgggtgtggc cgggaacgcc gacgccgatc ggtgccacct gggacggcca gggggtcaac
     7261 ttcgccctgt tctccgagca cgccgagtcc gtcgacctgt gcctgttcga cgcgcccgag
     7321 caggagcacg agagccagcg gatccgcctg cgcgaacgca ccgacttcat ctggcacgcc
     7381 tacctgcccg acgtacgccc gggccagttc tacgcctacc ggctgcgcgg gccctacgag
     7441 ccggaggccg ggcatcggtt caacccctcc aagctgctgc tggaccccta cgccaaagcc
     7501 gtctccggct ccctgcgctg ggacgacgcc ctgtacggct acaccatcgg acaccaggag
     7561 gaggacctgt ccagggacga ccgggacagc gcccccttcc tgcccaagtg catggtggtc
     7621 gacccggcct tcacctgggg tctggaccgc ccgccccgca cgccctggag ccgcaccgtg
     7681 atctacgagt gccacgtgag gggcatgacg atgctccatc ccgccgtcgc cgacgagctg
     7741 cgcggcacgt acctgggcct ggcctcggac ccggtgatcg accacctcct ctcgctgggc
     7801 gtgaccgcgg tggagctcct gccggtgcac cagagcgtgg ccgaccggca cctggtggag
     7861 agggggctca ccaactactg gggctacaac tcgatcggtt tcttcgcccc gggcgcccgc
     7921 ttcgccaccg gcgaccgggg tcagcaggtg gccgagttcc aaagcatggt ccgcgccttc
     7981 cacgaggccg ggctcgaggt gatcctggac gtggtctaca accacacggc cgagggcaac
     8041 cagctgggcc ccacgttgtc gttccgaggg atcgacaacg ccgcctacta ccggctgatc
     8101 cccgacaacg gccgctacta catggacttc accgggaccg ggaacagcct caacgtgctg
     8161 catccgcgca ccatccagat gatcgccgac agcctgcggt actgggtgca ggagatgcac
     8221 gtggacggct tccggttcga cctggccccg gtgctgggcc gtgagccgct gatggtggac
     8281 ccgggaggca cgttcttcga cgtggtgatg caggacccgg tgctgtccga ggtcaagctg
     8341 attgccgagc cctgggacct gggccacgac ggctaccagg tgggccggtt cccacccggc
     8401 tgggcagagt ggaacggacg gtaccgcgac acgatccggc ggttctggaa gggggagccc
     8461 gggcaggtcc ccgagctcgc gtcgcgcctg tccggctcgg gcgacatcta ccagggcagc
     8521 ggccgccgca cgtacgcctc ggtcaacttc atcaccgcgc acgacggctt caccctgaac
     8581 gacctggtga gctacgagca gaagcacaac gagcagaacg gcgagagcaa tcgggacggg
     8641 gcgaacgaca actacagccg gaactggggc gtggaggggc ccaccgagtc cacccgcatc
     8701 acgtcgatgc gcgagcggat gaagcgcaac ttcctggcca cgctgctgtt ctcgcagggc
     8761 gtgacgatgc tgctgggcgg ggacgagatc ggccgcaccc agtacggcaa caacaacgcc
     8821 tactgccagg acaacgagat cagctggccg tcgtggaacc tgtcccagcg ggaccgagag
     8881 ctgctgcgct tcacgcggtc ggtgctccgg atcttccgct ccaacccggt gctccgccgc
     8941 cgcagcttcc tgaccgggca gccggtgggc ggcaacggca cgaaggacct cacgtgggtc
     9001 cgacccgacg gccacgagat gcagggggag gactggaacg accccggcac ccacatcctg
     9061 gggatgctgc tctcgggcca cgccaccgac gaggtggacc agcgcgggcg ccccgtggtg
     9121 ggcagcacgg tgctgctgct cctgaacggc ggcgcgcgct cccgacggtt cgtgctcccc
     9181 aaggtggagg gtccggggct ctggctggag ctgatcaaca cggctcggcc gggatcgcgc
     9241 gtggtgaaga ccggcaccgt gaacctggtc gcgcactcgc tgctgctact gcggttcggc
     9301 gaggagcgca cccggaggtg agcgcccgcc cggccggagc cccgcgccgg cccgtcggca
     9361 cgtaccggct ccagctgcgc cagggggtgg gcttcgccga cgcccgtcgg ctgctgccgt
     9421 acctgtcccg gcttggcgtg agcgatctgt acctgtcgcc acccttcgcc gcccagccgg
     9481 gcagcgccca cggctacgac gtgatcgacc ccaaccggct ggatcccgcg ctcggcggcc
     9541 ggcgggcctt cgaggcgctg gcgcgggagc tccgctccag cgggctgggg ctgctggtgg
     9601 atatcgtccc gaaccacctg gccgcgacgc cggcgaaccc gtggtggcgc gacgtgctcc
     9661 gccggggccg aggatcgccg ttcgcgcggt tcttcgacat cgactgggac gcgcccggag
     9721 ccgacggcaa gctcctgctc ccggtgctgg agcgcccgct cgaggagctg ctggccgagg
     9781 gggccgtggg gctcgaggag gaggggcgtg aggtggtcgt ggccctcgcc gggctccacc
     9841 tgccgatctc ccccgcctcg gagcgggcgg tgcgcgagcg cggcctcggg gcctggaacc
     9901 ggggccgggc ctcgccccag agcgcccgga ccctgcggga gctgatcgag gagcaggcct
     9961 accgcccgct ctggtggcgc gagggcgacc gccgggtcaa ctaccggcgg ttcttcgaca
    10021 tcgcgggcct ggccgggctc cgagaggagg acccccgcgt gttcgaccgg gtccacgccg
    10081 cggtggtccg tctggtccag gagggaacga tcaccgggct ccggatcgat cacgtcgacg
    10141 gcctgctcga cccggaggac tacctgctcc gcctgcgacg ggccactcgc gtcccctacc
    10201 tcgccgtgga gaaggtgctg caccgggacg aggtcctgcc cggctcctgg ccggtccagg
    10261 gctccaccgg ctacgcgctg ctgaacgacc tgaccgatct gaccgtggat ccgcaggggt
    10321 ggcgcgagct cggagcggtg accgagcggc tccgcggcga cccggccccg ccgttcgagc
    10381 ggatccggct cgaggcccgc cgccaggtgc tccgggagct gttcgggagc gaggtccagg
    10441 ctctggtgcg ccgggtggca cggctggcct cgggcgccgc cctggacctg gaggaggggg
    10501 cgctccggga ggcggtggtc gagctcacgg cggcgctcga gggctaccgc acctacgtcg
    10561 ggcccgaggg ggccggaccc gaggaccgcc ggcggatcga gcgcgcggtc cgggccgccc
    10621 gagggcacct gccccggagc gcccgtccgg cgctccgatt cgtgcaccgg gtgctgaccc
    10681 tcgagctgcc gccggtggcc acggcgcgga cgcgctcggc atggctggag gtggtggagc
    10741 gctggcagca gctctcggga cccgcggcgg ccaagggggt ggaggacacg gccctgtacc
    10801 gggaggtccg cctgatctgc cgcaacgagg tgggcgccga cccggggaga gagcccgccg
    10861 acctgcccgg gttccaccgc cggatccgcg agctccacga gggcccccat ccgctcatcg
    10921 ccacctccac ccacgacacg aagcggagcg aggacgtccg ggcccggatc gcggtgctct
    10981 ccgagctgcc cggggagtgg gagcggcgcg cggcgaggtg gatccgggcc aaccgccggc
    11041 accgccgcgg cgtgcacggg cacgacgcgc cggatcgcga cgaggagctg ctcctgtacc
    11101 agacgctgct cggtgcctgg ccccaggacg aggatctggg gcccttcgcc gagcggatcc
    11161 gcgcctacgt ggtgaaggcc gcccgagagg cgaaggtcca cacgagctgg ctcgatcccg
    11221 atgccgccca cgagcggacc ctggaggcct tcgccaccgc cgtgattgcc gaccagcggt
    11281 tcctccgcga cttcctgccg ctccagcgca ccgtggcgtt ccacggggcc ctgaactcgc
    11341 tgtctcaggt ggtgctccgc actgcgctcc ctggcgtggc cgacctgtac aacggcaccg
    11401 agacctggga cctcagcctg gtcgaccccg acaaccgccg cccgctcgac atccgggccc
    11461 tggcccgcag gctggccgag ctcgaccgcc ggcagccccg ggaaggggcg gacctggcct
    11521 cggacctgat cggcgcctgg cccgacggac tggtcaagct gttcgcggtg agccggtgcc
    11581 tgcgcctgcg ccgcgagcgt cccgacgtct tcgtcgaggg ctcgtatctg ccccttcagg
    11641 tccacggtcc cgccgcggcg cacgcggtgg cgatcgctcg acgtgccggc gaacgctggg
    11701 ctctcgcgat cgtgcctcgc ctgacgctcc ggctggcggg ccctggccga gcaccgatcg
    11761 gggagcggat ctgggccgag accgtggcga tgctcccgcg cggagcgccc cggacgtggg
    11821 tcgacgcgtt caccgcccgc tcggtcggac cggacgcccg gggagcgctc gccctaggcc
    11881 aggtcctcgc cccgttcccg gtggcgctgc tggttcccgc cgagtgaggg cggtcggcct
    11941 cagcgatgtc gcgcgtgcgg ggcgtcggcc tcgaacagca gctccaggta gtggcggagg
    12001 tggctgagct gcgttcgcag gtagccgggg tcctcggtcg cccgggccag gatgaatgcc
    12061 ccctcgaaga tcgtggagac ctgatccgcg agcgagggca ggtccacgtg aaccgccgga
    12121 gggtgcgcct gggccgcctg ccggagcttc tccaggatcc gctcccgcca cagccggatc
    12181 gagcccacga tgatgtcgtt gacgtcctgg gacgagggca tgtgctcgta gatgaacgag
    12241 acgaacaggc atcccggctg gctgagcgcc agctcctcgg cggtgccctc gaaggcccgc
    12301 acgaagccga tcacctgctc ggcgggatcg tccgagtcgg cctccgcctt ctgcatgagg
    12361 gtctccagga cctccgcgtc gccggccgca tagcgctcca ccagagcccg ccccaggctc
    12421 gccttggtcg ggaagtggtg gaagaaggcg cccttcgacg cgccggccgt ttcgagcacg
    12481 gcatccaccg tggtcgccgt gaacccccgc tccaggacga gccgctgggc tgcgtccagg
    12541 atccgttcgc ggagggagct cggcggcgcg accatcggac gcgactatac ccgatttgac
    12601 agaccgaccg gtcggtctct agggtcggac cgaccggtcg gtctatcaag gggagggatc
    12661 gagcgatggc gagcgacacc acgacggtgg aggcggtacc ggcttctgag gcagggaccg
    12721 acggatcggc cgagaccacg ttccccgaac gggtgctcgg gtcgatcctc ggcacgatcg
    12781 acgtcttcac gatgtacctg ggggagaagc tcgggctgta ccgggcgctc ggtgaccacg
    12841 gcccgctcac gccgggaggg ctgagcgagc gagcgggcgt tcaccctcgc tacgcccggg
    12901 agtggctgga gcagcagacg gtgagcggga tcctcgcggt cgacgatcca cgccgcgagc
    12961 cgtcggagcg tcggtactcg ctgcccgcct cccaggccga ggtgctgctc gatcgggaca
    13021 gcctcgacta cctggcgccg ttcgtccgcg tcgtgatggc cggcggcctt gccatgccgg
    13081 cgctgatcga ggcgtaccga tcgggtggcg gcgtgagctg ggggcagctc ggggaagacg
    13141 cgcggagcgg acaggccgag atgaaccgac cctggtacct ccggtccatc ggcaccgagt
    13201 ggttcccgaa gatccccgtc ctccacgagc gtctggcggc cggtgcccgg atcgccgaca
    13261 tcgcgtgcgg cgaggggtgg tcctccatcg ccatcgcgct ggcctacccc gccaccacgg
    13321 tggacggcta cgacgtggac gagccctcga tccaggctgc ccgccgccat gccgaggaag
    13381 cgggcgtggc cgaccgggcc cgtttccggg tggccgacgt ggccgccgtc gacctcgagg
    13441 gcgtctacga cgcggtcgtc gggttcgagt tcgtccacga cctcccgcat ccggtcgagg
    13501 tgctgcgggc gatgcgccgg ctggtcaagc cggacgggcc ggtggtggtg ctcgacgaga
    13561 acgtccccga tcgcttcacc ggggagccca acgatgtcga gcgcgtgatg tacgggttct
    13621 cggtcctggt gtgcctgccc gacgggatgt cgcatcggcc gacggtggcg accggcacgg
    13681 tgatccgcgc cgacacgatg cgcgcctacg cgcagcaggc cggattcgcc gacgtcgaga
    13741 tcgagccgat cgaccacgac ctgtggcgct tctatcgcct cgtgcacgag ggcatccaga
    13801 tgggctgacg ccgaggcacc aggtacccgc tagccccccg ccagctcctc gtacaggccc
    13861 agggtccggc gggccacctc ggtccaggtg aactgctcga gcacccgctg gcggccggcc
    13921 tttcccatca cctcggcccg gcccgggtcg gccagcagct cgttcacgcc ctccgccaac
    13981 ccgcgggcga agccctcggg atccttgggc cggctctggt cgcccggctc gaaggggacg
    14041 aggatcccgg tctcgccgtc caccaccacc tcggggatcc cccccacagc cgtggccacc
    14101 acggccgtct cgcaggccat ggcctccagg ttcacgatgc cgagcggctc gtagatcgag
    14161 ggacagacga acaccgtggc gtgggtcagg agctggatca ccgagggctt gggcagcatc
    14221 tcgcggatcc agatcacgtt gccgcgctcg gtccgcagtc ggtcgacctt ggcctcgcac
    14281 tccgcgccga tctcgggcgt gtccggtgcg ccggcgcaga gcaccagctg ggcctggggg
    14341 tcgatctgcc gggccgcgtc cagcaggtag gtcacgccct tctggtgggt gatgcgcccc
    14401 acgaagatca cggaagggcg gtccgggtcg atcccgtgcg ccgccaggac ctcggtcccg
    14461 tcatcgggcg cgtactcctc ggcgtcgatg ccgttgtgga tcacccggac ccgcgcgggg
    14521 tccaggtccg agtacacccg caggatgtca gcgcgcatgc cctcggagac ggcgacgacc
    14581 gcgtccgcat gctcgagcgc ggtgcgctcg atgaaccggg tgagctcgta gccgccctcc
    14641 ccgagctgct cgctcttcca ggggcggagc ggctcgaggc tgtgcgtcgt ggccacgtgg
    14701 gggacgccgt gcagcagctt ggccacgtgt ccggcgatgt tcgcgtacca ggtgtggctg
    14761 tgcaccagcg aggcgccccc caggtcggcc gccatggcca ggtccaccga gagcacgccg
    14821 aggacggagg gcgcgccgga gagctgctgc catggccgga aggcccggac cggcggatgg
    14881 gcccctgggt cccggtccgc tccgaagcag cgaacctcca cctcgatcag atcggccagg
    14941 tcgcgcgcca ggtactcgac gtgcacgccc gctcccccgt acacctccgg cgggtactcg
    15001 cgcgtcatca ggaccacctt catcgttccc gttccccccc cgtggagcgg ccgtaggtcg
    15061 ccgtgatcac atcaccgcga ggccggagag atcgctcatg agatcgctca tcgcatccgg
    15121 atcggtggcc acgtcgacca ccgccggcac gcccttcgcc gaggcctcgg tggcgcgctc
    15181 cagggccgga cggaggccct ccgccgcctc cacccgttcc ccgtggccgc ccacggcctg
    15241 cgccagcagg tcgtagcgca tggaggagac gtcgctgtgc agcaccgact cctccccgta
    15301 gaacatccgc tgctcgtggc ggacgtcgcc ccacccgccg ttgtccacga ccaccacaac
    15361 cacgccggcc ccgttgcggg ccgccgtgtc gagctcgatc gccgacagcc cgaaggcgcc
    15421 gtcccccgtg aagacgaaca ccggctcggc cgggcgggcc accttcgccc cgatcccgaa
    15481 gggcagaccg ataccgaggg ttccctgcgc cgatccgatg aacaggttcg tgccgggagc
    15541 gtgggccgtg gacagggcga tcccccagag cacgctgtcc ccgccgtcgg agacgaaggt
    15601 gtgcgggccc tgctcccgcg cgaacctggc cacctcgcgg gccagccagc ccgggtggat
    15661 cccgttggcc ggacgatcgc cctccatctg ccacagctgt cggctagagg ccacgccctc
    15721 gcggcactgc tccatccatc cgtccaacgc ctctgccggg ggctgccagg catcggtcag
    15781 ggcctccagg gtggtcgcca cgtccccggc cagcgctacc tgcggccggc gccagccgcc
    15841 caggtgctcg ggccggacgt cgacctgcac cacccgctgg tcgggctgga acagcggagc
    15901 gccgccgtag gtcacgttcg cgttgaaccg ggagcccagg atcagggcaa cgtcggccga
    15961 ggccagcgcg atcccaccgt ggaccagtcc ccccaggcac cgtgggtggt cgtcgtccag
    16021 cagtcctcgg gccgcgctgg tggtcgtcac ggggaccccg gtgcgctccg cgaagttcct
    16081 cagcgcctgg cccgcgcccg agaagaacgc tcccgagccg gcgagcgcca cggggcgctc
    16141 ggcctcggac agcaggtcca tcgcccggcg caggtcgctc ggggccggca ccgagcgggc
    16201 gggctcggct tcgtggccca ccggccaggg cgcctggtag ggctcggtgg tagcggccag
    16261 cacgtcctgg ggcacctcga ggtaggccac gcccggcgct ccggcgcggg cctgatgcag
    16321 cgcctcggag acgaaccagc ccacgcgctc gggcttcagg cattcggccc gccacttcgt
    16381 gagcggtgcc acggcggcca gctggtccat gtcctgcacc gcaccgcgtc cccgctgctc
    16441 gatgccggtg cgcccggcga tcacgaccac cggcaccccg gccgcgttgg cctcggccat
    16501 gccgggaagc gcgttggcca gcccggggcc ggccgtgacg gccgccaccc cgacctgggc
    16561 ggtggccagg gcccaggcct cggccatgaa tacggcgttc tcctcgtgac gagcgcccac
    16621 gatgcggacc cccgtatggc gggcgccgtc gtacagggga gtgatatgcc ctccgggcag
    16681 cgcgaagatg gtgtggacgt cgccctgctc cagcgcgcgc gcgacgtgca tcccaccgtg
    16741 atcggtcgta ctcatgtggt gtggcgagta taggcagacg gcggacgaac ccggtccggg
    16801 tggtgcgtag acggccgcca tcgatgggta tgcggagaac gcgggctggc ccggccagag
    16861 gaggatgcca tgaccgagat gcagatcacc gagctggagc ccgatcggta cgcggtggag
    16921 ctgaccgagg gcaacgagca caccagccac gtcgtcgagg tgcccgagga catgctcgac
    16981 gacctgggcc tggtggacgc cgaccacgag gaggtggtcc gcgagtcctt cgccttcctc
    17041 ttggagcgcg agccggccac ctcgatcctg tccgggttct cgctggagca gatcgccaac
    17101 tacttccccg agtactacga cgagctgaag ctgcgcctga gcccctgagc tcttgagcgc
    17161 ccggcccgtg gccgctcagc gcagcgtgaa gatcggcctc ggctcgttgc ccgggcgcca
    17221 cttgatgctg cagccgacgc tcgggcgctg gtcctcgggc acgggccggt cggccagcac
    17281 ggcgtcgatc gcggaccgca ggtcctcccc ggtcaccggc acaccgttgc cgggacgcga
    17341 accgtccagc tggccccggt agaccagacg ccgatcccag tcgaacagga aggggtccgg
    17401 ggtgcaggcc gccgtgtagg ccatggccac ctcctgcctc tggtcgaaga ggtaggggaa
    17461 gtcgaacccc acctgcgcgg cctgctcggc caggctctcc ggggcgtcct ccgggtacgc
    17521 gtcggggtcg ttggcgctga tccccacgat ggccagcccc gagccccggt agtggtgggt
    17581 cagcccggcg atgccgtcct gcacgtgcac gacgtagggg caatgccggc acaggaacag
    17641 gacgagcagg gcgtcgtgat cgtcgagatc ggaccggcgc accaaccggc ccgtggtgac
    17701 gtccggcagc tcgaagtcgg gtgccctcgc tccaagctcg agcatgctcc agatcctggc
    17761 cacgctccat cgcctcccgt gtcgttcgtg tccctcgtcc gatgctagcc agacatgccc
    17821 ggctgcgccg ccggacgctc catgcccaag cgcacgagtt cggagagcgt caccggccgc
    17881 agaccccggg tcgacaggcc ctgcaggatc gcgggcacgg ccgccagggt ccgcagcggg
    17941 gcgccgttct gcccccggcc gtcgtgcagg agcaggatcg cgccgggacg gacggcggcc
    18001 agggtcgcct gggcgatctg cgccgacgag cggtccgatc gccagtcgcc ggccgaggcg
    18061 gtccaaagcg tccgccgcag gccgagccgc tcgcacaccg tccaggccgg ggccgtgaac
    18121 cacccgtagg gcggacggta gaaggcgggt gcccgcccgg tggcgctccc gatcgcgtcc
    18181 cgggctgccc ggagcgatcg agcggtgccg accggacccg cccggcccat cgagtggtgc
    18241 gtgtcgccgt ggcaggcgat ctcgtggccg gcggctctgg cggcgcgggc caggtccggc
    18301 caccgacgga ccgaggagcc gagccagaag aaggtgcacc gggcctgccc gagggcctcc
    18361 aggaaccgtt ccgcgaaccg gggatcgggt ccgtcgtcga aggtgagcgc cacggcgggc
    18421 tcctcggtcg acacggcggt gagcacccac cgcccgagcc ggcggcccac cgacggcacc
    18481 gcggccagcc ccggccccca gtaggcccct cccagggcca gggcgcccag cgccagccgc
    18541 cgccctgtgc tcacgccgcg gactcccgat cggcgccttc gcgctcctcc tgctcgaggc
    18601 gctggatctt gcggaccagg ggcgacgtgt cgagcaggac gatcccgcag aagaacatcg
    18661 cgaggccgaa gccggtcccg accaggttcc aggtgccggt ggcgatgggc tcgccgaaca
    18721 gcaccacccc gatcgcgatg gccaccgacg gctggaacgc gtccaccacc ggcatgctgg
    18781 cggccagcgg gccggcctgg taggccacct gcatcagcca gatcccgatc acggtggccg
    18841 gcacgatcgc gtacccctgc cagtgggtga acagctccac gaaccgggca tcagccagag
    18901 aggtggccga gtccaggaag gccgactgca gccccagcac gctggccgcc gcgatcgcga
    18961 ggatcgaggc ctgggccggg ccgcggagcc gccgaccccc caggacggcg gcggccgcca
    19021 gcaccgccac gaccccgatc gtgatggccc acgggacgat gtccgggagc gggttgccgc
    19081 gccggggcga ggcggcgacg agccccaccg ccagcccacc ggccacggac aggatcccca
    19141 cccactcgcg gacgcccatc cgccggtggt gccgccggat cgagaagggg acggcgaaga
    19201 tcagctcgga cagcaccagc ggctgcacca tggacagcgg cccgaaggcg agcgcgacgg
    19261 cctggaagac gtaggacagg aacccgaagg ccatggcgag cagccacacc cggtcggtca
    19321 ccagctgctc catgagccgg atcttgaacc ggccctgctc gggcttcgag ctcgcctcgc
    19381 gttgctccag gtagccgttc acggccaggg cggcgccggc cagggtcgcg aacccgatcg
    19441 tgatcgcgta agccccgagc gacgtcgcca ccgcctcgct acccggtgag tcgacgcgac
    19501 ggcaacgggc gcgggccgcg gcggatcgca tgaggcacgg ccacacgggg gcgctcggtg
    19561 atccggatca gctcctccag ctccgcggcg gcatccgcca cgaacaggga ccgtccacgc
    19621 ctggagcgcc ggcggcgttc cgcgggcgat ccggccagcc gggtgagggc agcgaccaga
    19681 tcgccctcgt cctccacgac ctcggtcaga cccagccgct gcatctggag cgcgtcgtcc
    19741 cgtccgtgtc ccgggatcgg ccggaacgtc acgacgggca gcccggcggc cagggcctcc
    19801 ttggaggaca ggcctcccgc gttatcgatc agcacgtcgc agacccgcat cagcccggcc
    19861 atgtcctcga cccacccgag cgccgtacag ccggggacgc gttccatctg cccacggagc
    19921 tgctcgttgc ggccgcagac ggcgacggcc atccatccgg gcatcgaggt cacacccctg
    19981 gcggcgcgga gcatgtcccc tatgcccagg gaccccccca ccaccagggc ggaccggacc
    20041 gcctgcggga tctcaaccgg gaggggctcg tgccccgacc gaccgtcggg ggacaggaac
    20101 gccgaccgga ccaggggacc acaggtccgg gtgggacgct gggtccggga ggccaggtag
    20161 tcggccacgc tcgccgagat gcacaggtag ccgtcgatgt ccgggtgcat ccacagttcg
    20221 tggaccccga aggtgggcac gaacgagatc acggggcatc cgagcgcacc gcgtccacgc
    20281 agctcggcgg cggccaggcc ggcgatgtga taggtggaga ccaccacgtc gggctcgaag
    20341 gaggcgacca gccgacgaag cccgggcagc gccagctgca cggggatccg gacccgttcc
    20401 ccccgctcct gcgggtgctt gaagaaggcc tggaagatgg cctggtacac ccacggcgct
    20461 cgtcgcaggg tccagaagta gaagccggcg atccactgtc cggccggccg tggcatcagg
    20521 tcgtgcacgt cggccaaccg ggtccggtag ccacgcgatc gcaggcgcgc agcgagctcg
    20581 agccccacgc tgacgtggcc gctgcccatg gatgcggtga ggatcagggc ccgtcgcccc
    20641 gaacgggagc cccccagccc cggccgcggc gtctccatgg ccttccacgt acccacagat
    20701 gccgcgtggt agccacctcc tccgcgtcgc cgccgagctc ggagggggtt ccccgttacc
    20761 ggctgccgct cgggtccgag cgcggcccgt ggggcgctcc cacgatcagg tcggagcgcg
    20821 ccagcgcgac caccccggcg agcatgatca cgatcgccac cgcgtccagc gcgatcgccg
    20881 gcgcgccggt cggcaggctc tcggagaaga gcaggacccc cagcgcggcg ccggccaggg
    20941 gctcggccac gttcatggcg gggaacgagt cgcgcagcgc cgccacgtgg taggcggcct
    21001 gcgagacgat caggccacct ccgccccaga tccccaccag gtatagctgc cagctcagga
    21061 acccccccaa ccgccgctgc tcgaagatgg ggacgagcgc cttggtgagt ccgtccgcca
    21121 ggccgaaggc gaccccggcc gcggccccca gcaccagggc tcggatggca ggtggggagc
    21181 ggagcgtgat ggcatgagcg gccgctacca gcaccgccgc tgccccgaac gtgtacagcc
    21241 acctggtggc cgagggatcc ccacccgcgg cgggtggtcc tcgcatgacc aggaacagga
    21301 ccaagccccc ggtggtggcg accatggcgg cccactcgat cggtcgcatc cggcggcccc
    21361 cccacgcgct cgcgatgggc agggcgaaga ccaggttggt cgtgaggagc ccttcgacgg
    21421 tcaccaggct cccgaagctg agcgccaccg cctgcagcgc gtagccgaag agcatgaaga
    21481 aggttccccc cagccagatc gggcgccgga agagccaggt caagaccggt acgcgggtgc
    21541 cggcgtgctc gggcgcccgc gccgccgccc gctgatggag caccgcggct agggcgaagg
    21601 acagcgcggc caggagcgac aaggcgaaga ccacagcagg ctgatgcccc gttcgcgggt
    21661 gcggctaacg gaccacgtat gcgagggccc cgggcagggc tccgctcagg ccgtctcctc
    21721 ccgcatccgc gtgaagaccg atgtcagcag gatgaaggcc agatgctcga cctgccggtc
    21781 ggtctcggga gccctactgc ggtgggcccg cacgatctgc gaggctcggg tcaccaggtc
    21841 cggatcctcc cccgcgacct cgtacagctc gtctgcgcgc ccactcaagc agccctccgc
    21901 gagctccatc gccttctcgg ccagctcttg ggccgtgacc atcgccctcc tccgcgaggg
    21961 ttcggggagc ccacggccgg gctcctcacc ggggacctat ccgggatggg ggcgatctaa
    22021 ccgcgtgggc cggttggctc accaacaggc gtgaggtcgc ttccctgggg ccactgcctc
    22081 gggcttcgcg tgcgggtcga acccgagcac gcgggtgcac cccgtcctgg accggtcgag
    22141 ccgcccggac gggctcgatg gcgcacggtc acgacggggc gcccggcgtc gaggcggggt
    22201 cgatcaccgc tagagctgcc accgatcgtc gatccccttg agggtgtggg tgccccggtc
    22261 ccggaaggcg aggtccgagc cgatcacaag gtccctgacc gtgttcgaga ccaggatctc
    22321 cgctggctcg gcgacgctca tgatccgcgc ggccagatgg atggcgatgc caccgacgtc
    22381 cccgtcgcgg agctccacct cgcccgtatg gatgccggca cggatcgaga tgtccactat
    22441 cgcgagctcg tcccggagag cggccgctgc gcggatcgct cgaccgggtc catcgaaggt
    22501 ggccaggatc ccatcgccgg tggtcttgac caacctgccg gtgttgccct cgaccacgtc
    22561 ccgcgcagag ccgtcgtgca ggtccaggac cgcacgccag cgccggtcgc ccagccgctc
    22621 cgcggttccg gtggagtcga cgaggtccgt gatcggcgat gtacctcgag agcgcctccg
    22681 gcacgaacgc gttctcctcg gcgtggacga cgagggtcgg cgcggcgatc gccggcagca
    22741 gcgctcgagc gtcggcggcc gccgccgcct cttggaactt ccggatcgcg ccggggctgc
    22801 tgatcaggcg ctggaacttc gcgaaccacg cccggaggcg ctcgtcgccg gcccggctcg
    22861 ggaacagcag gtcgaaggtt agaccggtgc cccagccctc ttggaagcgc gacatcgtct
    22921 cccgcaaccg ttccggcgac atcccgatcg ggtagtcctc gtcctcgagg taccgaaccg
    22981 ccgccatgaa cagcacgagg gcacggaccc gctcgggccg gctggccgcg aacagcaacg
    23041 ccgccggggg agcgggacca cccgcgatga tggccgcctg gcccgagccg gccgcatcca
    23101 tgaccgcctc catctcctcc gccaaggcct cccagggggg aagggcatcc aggggcacgg
    23161 gatcggaagc ccccgtgccg aactggtcga accggatgac cctggcgaag ctcgcgaggt
    23221 gctggaggaa gaggcgatgg gccggctcct cccactcgac gtcgaaggag ccgaagaacc
    23281 ccgccgccga ccgagagcac gggccaggca cggacgcgtc gcgtgaggga cagccgccgc
    23341 gcagcgggtt cggatggcga cgactccaga tcgccacggc gttcggcgtc ctggtctctc
    23401 tcgccctacc gatgttgatc caaggcagcg cggagatctt cctcttggcg atagcggcgc
    23461 ccttcgtcat cgggttgctc gtgatgcgcc gttcgccccc ggtgggcgcg atctggttgg
    23521 gggtctcgtc cctggcggtc ctgctatcga gcgcgccgtt cctcgctgac gcgttggccc
    23581 atcccgagtc cctgtcagac ttcgtccctc tcgtcatctt cggcatcagc acgctggtag
    23641 gggtggtcgc gacgatccct tctttccgcc tgggcgcggg ctcggatctt cgctcttcgg
    23701 ccgctcgctc gatcggggcc gttgcggttg cctcggtttt cgttgcgatc ctagtgtcgg
    23761 tcgcctcgtt ctcggggatc gagagcgcgc ctgcacggcc gggtgatgtc cgcttggtcg
    23821 cccaggacct caggttcgtt cccgcccaga tcgaagcgga tggtgggcgg atctccgtct
    23881 tggtcaccaa ccgtgacagc acgcgtcaca cgttcacggt cgacgagctc gggggtggac
    23941 ctgagcatcc cgcccgactc cacgcagagg gtctcgttca cggcagatcc gggcacgtac
    24001 cgcttctact gccatccgca cgccccggga atggagggtc ggctggttgt gagatgagcg
    24061 gtgtggctca tccctgggtg ggccccccgg gactcgaacc cggaacctgc ggattaagag
    24121 tccgttgctc tgtccagttg agctagaggc ccgcccgact ataccaaccg gccagccctg
    24181 ctcagtgggg tgaccgaggg gacttgaacc cccaaccccc aggaccacaa cctggtgctc
    24241 taaccgattg agctacggcc accacgtgcg ggccgattct accagtgggc ccctgccgac
    24301 ccggtcctcg cccggcccac tggtcgccgg tccggcaagg cttcggcgga agtccagcgg
    24361 atggttaggg aacggtgagc ggtgaggagg tccctccgta gcatgaccct cgagcgcgat
    24421 ccggccgggt cgacggtggt ccagcgatca gcggaccacc cgtgcgacga cgacccaagg
    24481 atgagcatgc ccatgcgacg gagcatcatc gtcttcgtgc tggcctggga accgccatgg
    24541 tcctgccggg cgcggcctac gcgaagggcc cgccggccac ctcgttctgt gtggacggga
    24601 ccctgtactg gaccgtcggg acgccgaccg acctgtccaa cacgggcgcc cccgactcgg
    24661 ccttcgacac gatctacgag ttcttcgggg cccagcccga caacgtggcc accgcggccc
    24721 ccggcgaccg ggatcacaac ggcggccggt ggcacgtgca cgggctgtcg ttcccggacg
    24781 gctacgccgc ggcactgtcc agcggtgacc ggaccacgac ggcgtcatcg acgacgacac
    24841 cgagatgcag gcggcgcttc ggagcgggga ggcggtggac ctggggatcg tgatgtcctt
    24901 cgagtgtccg gtgatcccgc tcggcggcca ctaaggaccc gcgagcaaat aacattgatg
    24961 tgattcagcc ggtccggccc ggtctgatcg tgtagttcca ctcgccgtgg aacctgtggg
    25021 gcacgagcgg tatggcagcg aactgctcat cggagacctt gacctgcaac gggtaggctc
    25081 cggcgtcccg ctcggcgtgg acggccagtc cggttgccgt ggtcgtggcc ccgatcagct
    25141 ccaccaccac ctcatggctg gtgagggggc gaccccgcca gttcatggag atgtgggcga
    25201 agaggcgatg ctcgatcttg ttccacttgg aggtccccgg cggcaggtgg cacacggtga
    25261 ccgacaggcc ggtctcggtg gcgagccggg agagctcgag cttccacagc ctgacccggt
    25321 agccgttcga tcctcccggg tcggcgcaga tgagcaggcg cgtggaccgg gggtaggcgt
    25381 cgcggccgac cccgttccac catctccgca cagcctccac ggcgaagctc gcggtgtcgt
    25441 ggtccgagcc caccgagacc cagccggtgt tggcccccac gtcgtagatc ccgtacggga
    25501 tggccttgcc gacggccggg gagatgaagt cgtagatgtt cacctcctcg ggctcgccct
    25561 tcggctgcca ctcccgcccg ccgttcttgt agctccccac cagctccttc ttcttggtgt
    25621 ccaccgagat caccgggtct cctcggcgca gatgcgctcg gacccgctcg ttcacgtagc
    25681 ggaactgggc gtcccggtcg gggtgatggg tcccctccat cgtcttgcgg ttggcctgca
    25741 ggctgtagcc cgcccagtgc aggagcttgg ccacggtcca ggagctcacc gggtgcccgg
    25801 tctcggtgag ggcctgagcc agctggcggg tgctcttgca ggtccaccga agtggcgaca
    25861 tcggatcgcc gcgggtggag gggtccacca gctcctccag ggcctgcagg agggcggggt
    25921 ctttctccgc cagctgcttg cgacctcccc ctgggcgacg ggaccgaccg gaggtggggg
    25981 cgggctcctc ctccagttcc ctgatggcct tgcggacggt agggcgggag accgaggctg
    26041 cccgcgccac ggccgccact cctccacggc ccagctcccg ggcctccgct gcgagcaaca
    26101 ggcgacgctg acgctcgttc aggtg
//
LOCUS       DASQQQ010000019        30940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_56117, whole genome shotgun sequence.
ACCESSION   DASQQQ010000019 DASQQQ010000000
VERSION     DASQQQ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 30940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30940
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56117"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VE646_00890"
     CDS             <1..737
                     /locus_tag="VE646_00890"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HZD78584.1"
                     /translation="ADCAYLVDKFSRGFDHLIYVWGADHHGDVVRVRGAAEALGFDPD
                     AVEMILYQWVALYRGGRTVGMSKRAGTLVTLDELLDEVGADATRFTLLSRSNDATIDF
                     DIEAVKQRSMENPVYYVQYGHARIASILRHAAQRGLDLLPVEEADLSQLRTESELDLL
                     RAVAEMPEQVAQAATLRAPHRLTHYAQALAAQFHRFYTECRVVTEDRALTQARLWLCT
                     GTKQTIANVLGLLGVSAPESMERTDA"
     gene            730..2571
                     /gene="lysA"
                     /locus_tag="VE646_00895"
     CDS             730..2571
                     /gene="lysA"
                     /locus_tag="VE646_00895"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZD78585.1"
                     /translation="MRDALDRPSAPGDPWPSAATFGPEGLRIAGARALELAERYGTPL
                     LVVDEEHLRARCRAFAELFPHPLYAVKAFTSRGAIRIAAEEGLDLLAATRGELEACLR
                     AGVPAERIVLHGNNKSERELHLAVDEGIRLVNVDNRDELQRLSAIAEAAGVEQPILIR
                     VVPEIVAGGHEKIRTGHRGSKFGLPLEEVPDAVSEAVALPGVRPLGIHAHVGSQVLEP
                     GPFLEAVDVLLDLLVRVREVSGCEAEVLDLGGGFGVAYTDESPPSLETLAPALLGRVR
                     EGARSRGLAVPHVLVEPGRAVVGGAMVTLYRVGAVKRAGERLLVAVDGGMSDNIRPML
                     YGARYTVAPAGTERPGGPTTVDVVGKHCESGDLLARDVRLPWPPQRDDLLAFAATGAY
                     TYSMASNYNRVGRPAVVAVRRGEVRPWLRREDPADLDRLEVALEDRPMPEPPAEVEVR
                     PARPADTDSFLDAYRSVAAERRFIQTEVVTGTPRQYRRTFRRAWTSEEATLMALAAGR
                     VVGSLSIRRDPQGATRHVATLGMFVLDGWRGRGVGSALMAEALRWARAQGVRRVELTV
                     YPHNRAALALYRRFGFVEEGRLIRHARKSYGYEDEILMAVWLGSEGA"
     gene            2568..3857
                     /locus_tag="VE646_00900"
     CDS             2568..3857
                     /locus_tag="VE646_00900"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HZD78586.1"
                     /translation="MIDRRLRVGVLGAGTVGAAVIRLLERHRGDITRRAGCELEVASV
                     AVRDPAKARDVPLDPPRSTTDPMEVVDDPSIDIVCELIGGTEPAGSLILAALERGKPV
                     VTANKELLANRGREILDAADAKGLDLFFEAAVGGGIPLIRPLKESLTGERLSRVMGIV
                     NGTTNFILSRMAEEDRPFDEVLTEAQRLGYAEADPTADVEGFDAAAKCAILASIAFNA
                     RVVAGDVYREGIARVTNEDIGYARRLGYVVKLLAIAEFDGERIAARVHPAMIPASHPL
                     AAVRDAFNAVFVEGPNVGELMFYGRGAGGEPTATAVVGDLVAAARNLQNGTRGVGCTC
                     FWERTIRPMEELADQYYILLRVQDRPGVLAEISRVFGDNDVSIRSVWQEGGGADAKLV
                     FVTHRALEGNFQHAVAALDDLPAVEEVRSVVRVEAEE"
     gene            3854..4909
                     /gene="thrC"
                     /locus_tag="VE646_00905"
     CDS             3854..4909
                     /gene="thrC"
                     /locus_tag="VE646_00905"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZD78587.1"
                     /translation="MSARPWRGVIEEYRDRLPVSDATPLVSLGEGGTPLVRSDALSSE
                     TGCEVYLKYEGANPTGSFKDRGMTLAISKALEEGSKAVVCASTGNTSASAAAYAAKAG
                     LTSAVLVPKGKVALGKMAQTLIHGARVLEVDGNFDASLDLARDLSRHYPVTLVNSVNP
                     YRIQGQKSCAFEICDALGGAPDLHCVPVGNAGNITSHWMGYREYLADGLVAEPPQLFG
                     FQAKGAAPIVRGEPVPDPSTIATAIRIGNPASWEQAVAAARESEGAIAAVTDRQILAA
                     YRRLAREGLFVELASAASVAGLLQRSSDRQLPRGATVVCVLTGHGLKDPEWAITGAGH
                     PPTVPVDPAAVAVELGL"
     gene            4914..5798
                     /locus_tag="VE646_00910"
     CDS             4914..5798
                     /locus_tag="VE646_00910"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HZD78588.1"
                     /translation="MRLRAVVPATSANLGPGFDCFGLALDLCNEVTLDTEARPGVRWD
                     GEGAQELPTDGTDVVSRAIAAAVERRRRHHPEISAPPFGLYGVNRIPLERGLGSSSAA
                     AVAGTALAAALLGPVGWEDLPTTFAYAAELEGHPDNAAPAAFGGLTVIADGGVHRLDV
                     HRAIRPVVLVPQDVRTSTTRARRELPARVLLADAIYNVAHGALAVRALTSGDLDLLLV
                     SLRDRLHERIRLSSVPAVEETLDRLRRSQVPACVSGSGPSLLAFEGDGRPVPHPGPGW
                     QVLPLAVRATGAEVIREG"
     gene            complement(5795..6616)
                     /locus_tag="VE646_00915"
     CDS             complement(5795..6616)
                     /locus_tag="VE646_00915"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HZD78589.1"
                     /translation="MGRTTRAASRIRALILVAAMAAVPLTWGSLESSARPVASTAGIT
                     RGAGPALRPGRALAERAAATDRSSHRSQPGSALLVRVTARTPIRSEPGAGRLIGWVPA
                     VSRYYHAPIVAWVRRTSADGRYGLLAVPYSAGLRSGWIALRGMRRTGTRISVQVDLSR
                     HLLTVMRGARPVAHFPAATGAPSTPTPTGRYFVTDRVPFSPGSAYGSFAFGISGIQSH
                     LPAGWWGGDQLAIHGTSAPSTIGTSASAGCLRVSEQALARLRPLLRPGTPVIVRP"
     gene            complement(6704..7876)
                     /locus_tag="VE646_00920"
     CDS             complement(6704..7876)
                     /locus_tag="VE646_00920"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HZD78590.1"
                     /translation="MDALDVLLIALIGLAAYLGYRRGALLQVLSFGGLALGLLAGALL
                     GPPLASLARGDAIRAGIAVVVLLGLGSIGNALGWALGSWVRARARRSGLRPVDAGGGV
                     LVSMGAALLAIWFVALNLVNGPFPAVASQIRGSAVVRGLDASLPRPPSLLGEIRRFFN
                     RYGFPDVFLGLPPAPAGSVDPPSRAQARRVFAAASGSTLRIVGQACGEIQSGSGFVVA
                     DHYVMTNAHVVAGVMAPRVQAQGGASQSATTVMFDPRVDLAVLYVAHDPGPPLPLSGS
                     PAVRGARGAVLGYPGGGSLTGSGAAVRRSIDAVGRDIYGRQVVERSVYELQTTVQPGD
                     SGGPFVLTDGTVAGIVFAASTTDRDIGYAISSTAASSGLAHTIGSTRAVSTGACVG"
     gene            8000..8791
                     /locus_tag="VE646_00925"
     CDS             8000..8791
                     /locus_tag="VE646_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZD78591.1"
                     /translation="MVTIAHISDPHVGSPYFVPNLMNRVIVELNELAPDVVICSGDLT
                     NEGYRQEYKNWVAYAERIRAPLHTVPGNHDARNVGYLHFEELIGPRHWSIDLQGVRVV
                     GADSSEPDLNEGQVGRERYGWIREQFAVPADLKVFVLHHHLLPVPGTGRERSTVMDAG
                     DLLEELIGAGVNIVLSGHKHVPYVWRLENLYIANAGTVASLRLRGYTKPCYNVLRFEG
                     DDVKITRRFPFGGESIIAHFSLTSGEQFYREFEPPVQQTVAQTGE"
     gene            8819..10150
                     /locus_tag="VE646_00930"
     CDS             8819..10150
                     /locus_tag="VE646_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate synthetase"
                     /protein_id="HZD78592.1"
                     /translation="MRVLALVDGEHYPPVTRWGIRRAAERGLRVELALFLGGTEKVLP
                     GALPDLGVPVLPAGADAAGALASALGQHAVEAVLDLSDEPVVGYRERMELAAASLAAG
                     IPYLGSDFRLDPPIHGPPLSIPTVAVIGTGKRTGKTALGGEVARVAAREGLNPVLVAM
                     GRGGPPDPQVAPAGTVDLDHLLELVRRGEHAASDYLEDAVTSGVTTIGARRAGGGLAG
                     APFASNVREAAEMAVELGAGVAVLEGSGSAIPPIPWDAGVLVVPAFTPLEYVTGYLGP
                     YRLLLSDLVVLTMAQSSRAGLENLHALRSHVQRSRGAPRFVVTEFEPVPLGDVGGMDV
                     FFTTTAPEPVAARQVEVLEAGYGCRVVGWSARLADRAGLAQDMDEAEGYQVLLTELKA
                     AAVDIACERALASGAGVVFVDNRAVAVEGESDLPTLLRETIELARNRRAAR"
     gene            10158..11108
                     /locus_tag="VE646_00935"
     CDS             10158..11108
                     /locus_tag="VE646_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of 2-phospho-D-glyceroyl
                     phosphate from ATP and 2-phospho-D-glycerate; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphoglycerate kinase"
                     /protein_id="HZD78593.1"
                     /translation="MTKDLKSPHILISDREMGLPFSKGLLASQVMVTGLSPYRAYEVA
                     EAVEDRLLDLSRPSVTTEELDGVAIEVIQEIAGERYAKNFLRWREVDALDIPLVLLIG
                     GTTGVGKSTIATQLAARLGIVRTVATDAVREVMRAMFSHELMPTLYRSSFEAGDALRE
                     PPTRDAVVVGFREQVSAVAVGVDALVERAALEGMSMIIEGAHLVPGFMDLESRNERIL
                     AVPVVVTVEDEEVHRSHFMARSSDHQTRPATRYLRGFDNIRRVQRYIKSQALSHGVPV
                     IPNYNFDHTLAAVIDLVMERVTERASVLAPTANGTKGRGA"
     gene            11105..11767
                     /locus_tag="VE646_00940"
     CDS             11105..11767
                     /locus_tag="VE646_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HZD78594.1"
                     /translation="MRLFLDTANLEEIREINRWGVLSGVTTNPTLVGRENEDPGQLWK
                     QILAEVDGDVSLETTEPEATPMYEQGVRLAGMGPNAVVKVPMTPSGLEAGKRLVADGI
                     KINVTLVFSPAQAILAAEIGAYIASPFLGRIDDAASDGMHALAQICDIYAVQGYDTQV
                     LAASLRHPMHVVQAAELGADIATMPYDVFTKLVKHPLTAAGLQRFDEDWQTLQRGLKG
                     GA"
     gene            11786..13468
                     /gene="rho"
                     /locus_tag="VE646_00945"
     CDS             11786..13468
                     /gene="rho"
                     /locus_tag="VE646_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZD78595.1"
                     /translation="MDRGDLEGKVASELHTIADGLGIEGHQRLKKADLIERILAHSGG
                     GGNGAQAAAGAAAETAPPAETSSPNGEVASDRTTVQESPSQTSEAPPGVREEPRAEDR
                     SPRPRIERDTREDGGGRPEREDRGERPKGREPQGEPRRDRPEGRGGEGRQDTRGGEGG
                     RRRLSREERRRQREERRQREMEAREEEIANAPTATGLLDILPEGYGFLRTSGYLPGSE
                     DIYVSLSQVRRFMLRKGDTVTGKIRQPKDNEKYFALLQIEHVNGLEPEEAKQRAHFEK
                     LTPLFPHDRFRLETAPGDVTGRIIDMVAPIGQGQRGMVVSPPKAGKTTVLKNIANGII
                     ASSPDTHVIVLLVDERPEEVTDWQRTVEAAEVVYSTFDKPADQHIQVTELVLERAKRL
                     AETGKNVAILLDSITRLARAYNLAAPASGKILSGGVDSTALYPPKRFFGAARNIEEGG
                     SLTIIASALVETGSRMDEVIFEEFKGTGNMELRLDRALAEKRIFPAINVEASGTRKEE
                     LLMPTEELALVWRLRRVLHALEPGAALELLVDKIRATKSNEQFLKEIAKAPS"
     gene            13503..13751
                     /gene="rpmE"
                     /locus_tag="VE646_00950"
     CDS             13503..13751
                     /gene="rpmE"
                     /locus_tag="VE646_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HZD78596.1"
                     /translation="MKQDIHPEYMIATVHCSCGNTFQTRSTKPELRVEICSNCHPFYT
                     GKQKLVDTGGRVERFQRRYGKQAPTGGSAQARQPQGQG"
     gene            13760..14674
                     /locus_tag="VE646_00955"
     CDS             13760..14674
                     /locus_tag="VE646_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HZD78597.1"
                     /translation="MAAEAPAKQHFYGGQAVMEGVMMRGRDVWAIAVRRSDQRLHVES
                     HDIHSIAERHPIFSKPGLRGVIALGQALSIGVRALAISANQASPEEEQLSSRQMVLSM
                     VVAFAIFVAVFIVGPAAAFRWVGGHLRSSILVNALEGVFRVLLFLGYLLAIGRTSEIR
                     RVFEYHGAEHKTIAAYEHDEELVPERVDRYSTLHVRCGTNFLLIVMVLTILVFAFFGD
                     PGIWWRIGSRIVAIPMIAGLAFEMLRLGAKFERSTLMRALMAPGLWLQKITTRPPDHG
                     QIEVAVASFNEVLRREAEKVAMRAGLQV"
     gene            14755..15534
                     /locus_tag="VE646_00960"
     CDS             14755..15534
                     /locus_tag="VE646_00960"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HZD78598.1"
                     /translation="MSSSELDLPVLASPDQIRRREFVTTRRGYDPEQVRDYLEQLAAQ
                     MERLGTMAREARMQADAAQRAQAETPQADPYEELGRRMSNLFRTAEQEAERIGRESRA
                     EAEEIIREARAEAGRIRLDAQARAEEARDAADRALREARERADRTLAGLTTRRDALVQ
                     QLAAMQERLLGVARELEVAIDRDETHTVVLGEAGPAEEERSEHANVRAGGTEDRSGSA
                     AGTAPGGFEELWSRSEGDADLSIPDIPPLDLGWDEDDPGSS"
     gene            15582..16661
                     /gene="prfA"
                     /locus_tag="VE646_00965"
     CDS             15582..16661
                     /gene="prfA"
                     /locus_tag="VE646_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HZD78599.1"
                     /translation="MDLTERLDELEHRYEGLSGRMASREVASDPELLRSLGKDLAELQ
                     EVVLPYREYKAALHQADEARMLGSREPDQEMAAFLQEEEAAARGRATELRRRLEELLV
                     PKDPNDDKDVVLEIRAGAGGQEAALWAGELFELYRHYAERRRWKTEVLSASPSDLGGF
                     KEVVLEVRGRGAYSRLKHESGVHRVQRVPVTESSGRIHTSTATVAVLPAAEDVDVQIR
                     PEDLKTDVYRSAGPGGQSVNTTDSAVRITHLPTGIVVAVQEERSQLQNREKAMRYLRA
                     RLLQQAQQEAQAEEAEARRAQVGTGERAEKIRTYNFPENRVTDHRVKITLHRLPDVLQ
                     GDIDGFVDALMAAERARQLSGDGRG"
     gene            16667..17497
                     /gene="prmC"
                     /locus_tag="VE646_00970"
     CDS             16667..17497
                     /gene="prmC"
                     /locus_tag="VE646_00970"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZD78600.1"
                     /translation="MRPALVLRRATDYLERHGVESPERTAEVLLASVLGTDRAGLYAR
                     STGLSTAEARMFGRALCQRCSGTPLQHLTGDQSFRGIVLRVRPGVFVPRPETEILVEV
                     ALTELDRQEDPVVVDVGTGTGAIALAIKQERPGAAVYATDASMEAVRLATENASRLGL
                     DITVVEGDLLEPLAVELRGWIDLVVSNPPYVTAEEYETLPPEVRADPSSALLGGTAVH
                     ERLVQEALRWLRDDGVLAVEIGAAQGEAVWRVFAASYTSVRVARDLAGRDRVVVGRRP
                     "
     gene            17494..18105
                     /locus_tag="VE646_00975"
     CDS             17494..18105
                     /locus_tag="VE646_00975"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZD78601.1"
                     /translation="MRDRVEAAAAAALSGELVVLPTDTVYGIGTRPDDPAATERVFRA
                     KHRSPTLELPVLVPSPEVARGIAVFDRAAETLAARFWPGPLTLVLPRTERSRGWELGG
                     DGRSVGVRMPDHPLALALLQRTGPLAVTSANPSGEATPVDPGGLERVFGSAVSQYLCS
                     EPLPPAPPSTVVAISGTDLRMLRIGALSERAIRSALGATAGPM"
     gene            18132..18725
                     /locus_tag="VE646_00980"
     CDS             18132..18725
                     /locus_tag="VE646_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HZD78602.1"
                     /translation="MPHVLVVCTGNICRSPIAEGFLRAAFERRLGPQAPAVTSAGLVA
                     RDGNAAEPEAVRAAAEHGVDISDHAARRLTGRMMAEADLVICMTSGHRDRIVEALPDA
                     LARTFTLKELVFLLESLPDPAAHAPADRLSERVAAAHELREHSDRPVDEDIGDPLGLS
                     IPAFRATAIELEGLCTRLADGLFPEREERRAAGHGGA"
     gene            18729..19175
                     /gene="rpiB"
                     /locus_tag="VE646_00985"
     CDS             18729..19175
                     /gene="rpiB"
                     /locus_tag="VE646_00985"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HZD78603.1"
                     /translation="MRLAIGSDHAGFPLKGPLCEFLEGEGHEILDVGTNSEEPVDYPA
                     FCASVARAVAGGDVDRGIILGGSGQGEQIVANKVHGVRAALCHDLYTARLSREHNDAN
                     VLTMGARIVAATLAQEIVRVWLDTPFDGGRHERRVEQISMIERGEM"
     gene            19175..20440
                     /gene="glyA"
                     /locus_tag="VE646_00990"
     CDS             19175..20440
                     /gene="glyA"
                     /locus_tag="VE646_00990"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013288262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZD78604.1"
                     /translation="MGGPVADWGALERTDPEIARALTGELERERRTLRLIASENYTSR
                     AVLAALGSTMNNKYAEGYPGRRYYGGCRFVDVAEELAVARAKELFGAEHANVQPHAGA
                     QANLAVFGAFLEPYSDDKVLGLVLAHGGHLTHGSPVNVSGMWFDFVGYEVDPQTEQLD
                     MDRIRELAKEHRPKIVLAGYTAYPRTIDFAAFRSIADEVGALFWVDASHFIGLSAGGA
                     YPNPVPYADVVTFTTHKTLRGPRGAVILCREEHGRAIDRAVFPMMQGGPLENVIAAKA
                     VALKEAMEPSFAEYTHRTVRSARTLAQGLAEEGLRIVSGGTDSHLALADLRPLGVSGR
                     DAEVACDQIGIALNKNGIPFDPMPPLNPSGIRVGTPGPATLGMDEAEMKEVAAVIGRL
                     LRRPEDESTRAACRARVQELCDRFPVYPI"
     gene            20486..21697
                     /locus_tag="VE646_00995"
     CDS             20486..21697
                     /locus_tag="VE646_00995"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HZD78605.1"
                     /translation="MVPYLVVFGAAAVATFATTPLVRWVSLRVGWIDRPSDRKVHPNP
                     TPTAGGLAILLGIVTGLAVSRLVPFLRGLHETTSEPDAALLAALAIVLVGLIDDVKGL
                     SAAGKLVGQVLAAGILVLAGVQLLYFYLPIGGIGILSLSPDMAVPLTVVWVVATVNAV
                     NLVDGLDGLAAGMVAIAAMAFFAYMYRAPGVDGAPSTAALLSALVAGASVGFLPWNFH
                     PARIFMGDSGAMLLGLMMAAASISGVGRNQSPTGGDFAAVAIPIAVPLIILAVPFLDV
                     VLAIVRRVRRGAGITHADKEHIHHRLLDIGHSHRKAVLLMYLWSALISACALAVAFIN
                     GRMLVGGIVAVAVILAAAIPRIVMRRPAGVPIRRVRGDGQIEAGLSLDHGPARTPIPS
                     PEPPAAPHQGS"
     gene            21884..23011
                     /locus_tag="VE646_01000"
     CDS             21884..23011
                     /locus_tag="VE646_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD78606.1"
                     /translation="MAEIDVRLDRVTKDFGVTVAVDDLSLDISEGEFFSLLGPSGCGK
                     TTTLRMIGGFEEPTRGTIYLGGRDVTDLPPYRRDVNTVFQSYALFPHLTVFENVAFGL
                     RRRKVNKHEIDERVNDALRLVDLTGFENRKPGQMSGGQAQRVAVARALVNRPKVLLLD
                     EPLGALDLKLRKRMQLELKRIQGEVGVTFIYVTHDQEEAMTMSNRVAVMRGGRIEQVG
                     PPEDVYENPVTEFVASFLGASNLLDGEVKEERNGTVAVLLTGGDTVAVPAERAPSGLG
                     SKVKVGVRPEKITIRAEGAEPRQGWNGITGLLRMSTYIGVSHQYKVEGPGGAEITVWV
                     QNLGTEPVPRPGERVRLSWPQEHTFAVLPQEGLTLEEEEGA"
     gene            23008..24333
                     /locus_tag="VE646_01005"
     CDS             23008..24333
                     /locus_tag="VE646_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZD78607.1"
                     /translation="MNQRDDRTFLEPQRMQEVLRRRLTRRSLLKGAGTGAAALGLGGL
                     LAACGGGTSGGGGTNGGGGSTSSAFDVSQIYSGDPGSSINFANWPLYIDHAKDKNGNR
                     YIPSLRNFTKETGIDVSYQEVIQDNTDFFGKLQPQLQAGDDTGWDIIVITNGREFTAL
                     TKLDWVYPLDPGKRPNFDANAAAWAKNPPYDPDNTHGMAWQSGITGIGVNDKLVKGSV
                     TKLDDLANPDIVGVGKVGMLKNDMQDFVMINLGIDPTTSGPDEWKEAADWLRKQRDTG
                     VVRQYYDQGYVDDLTAGNLSATMAWSGDVLYYKLWAGYDNLDFVFPDGGALLWIDNMM
                     IPAHANNPVGAMELMDYYYKPEVATGVTEWVLYMSPVPATRDLIMKDSQAARDEGSKG
                     YANKLEATANSPYLYPDDAFLARTKFGRDLKTDEERQEWDSIFLPITQA"
     gene            24364..25275
                     /locus_tag="VE646_01010"
     CDS             24364..25275
                     /locus_tag="VE646_01010"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD78608.1"
                     /translation="MDSSTGAAQQGRVRGRSIGLRKKLVPYYLLGPGAVWLLIFLVLP
                     LLLLVYTSLQSGGLLGGFHFTWQFSNYADALSQNGKYFGRSLMYSSIVTVVALLLAFP
                     MTYWIAFYGGRWKSSLLLLMLLPFFVSFIIRTIQWKFILADNGIILGPLKNLGLLPQS
                     FHVLATPLAVVAGITYDFLPFTALPLYVSLERIDKSLVEAAKDLYASRWQAFRHVVVP
                     LSLPGVFAAFLLTFVPATGDYVNAAILGGPGTTMVGNIIQTKFLYQNVYFEAAALSAV
                     LMLGMFVLVSIYARVLGTEEVVRAAAA"
     gene            25272..26129
                     /locus_tag="VE646_01015"
     CDS             25272..26129
                     /locus_tag="VE646_01015"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD78609.1"
                     /translation="MSVVTAEREQRVLEPAPLHRPNKRWTRFLLPGFTGLMIAYLMSP
                     VFVMILYGFNNIPGERQTPKFWGFTIHWYKELFAIQGLTTAIKNSLIIAPTAAVVATI
                     IGTFLGLALGRYLFRGKGAVDFFIFLAIAVPEIVLGSSLLSMFVQARASLGLGTILLS
                     HIAFDVPFVAVTVQARVAGMDRSLEDAAQDLFADPVTTFFKVTLPQILPGIFAGFLLA
                     FVLSLDDFVITQFVSGQTETFPIWVWGSTRIGLPPQVNVMATLLFTAGVVISIANVAA
                     QRLRRSRAA"
     gene            26315..26494
                     /locus_tag="VE646_01020"
     CDS             26315..26494
                     /locus_tag="VE646_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78610.1"
                     /translation="MGTAWNVMGTLASGILVWGGIGYLVDRVVGFHWLFLPIGMVIGV
                     SASIYVVYLRYGRDH"
     gene            26478..26939
                     /locus_tag="VE646_01025"
     CDS             26478..26939
                     /locus_tag="VE646_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78611.1"
                     /translation="MAEITERAERELVRRALVPSAVAVPLSLLIGWALAGPGVGVSAA
                     IGIALVFANFAAHGLSLAWASRVSIVAVQVVALAGFVVRLGVVVAAMFVLNTLAWFSP
                     LAFGLAVVVATLSLLVYEARLVLRGMGGTLMIPADPVAAQAARALAAREAR"
     gene            26939..27760
                     /gene="atpB"
                     /locus_tag="VE646_01030"
     CDS             26939..27760
                     /gene="atpB"
                     /locus_tag="VE646_01030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HZD78612.1"
                     /translation="MLAALASGFAPAVAALSRPPEGFHAPDTAIFDYSNNCLFGNGSY
                     CITRPTFWLFVSVLIIAAVFIAAFRRPQLVPRGLQNAVESLIDFIRNGIVMEVMGPDG
                     LPYLPLLTSLFVFIWVNNTFEVIPFIQFPPTSKIAIPMILAGLVWFIFNIVGIVKHGG
                     WRYLKSIMFPPGVPPAIYILYTPIELVSTIIVRPVTLTVRLTANMIAGHLILTTFFLG
                     TWYLQWKLLTIPFAAASFALGTFITGFEILVSVLQAYIFTILTAVYIASSIHPEH"
     gene            27776..28024
                     /gene="atpE"
                     /locus_tag="VE646_01035"
     CDS             27776..28024
                     /gene="atpE"
                     /locus_tag="VE646_01035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HZD78613.1"
                     /translation="MSHLFGLLAQVTDTGVSSIGKGLVYGLGAIGPGIGIGYLVGKAV
                     EAMARQPEAAGMVRTTMFLGIAFVEALALFGFVLSFIV"
     gene            28046..28597
                     /gene="atpF"
                     /locus_tag="VE646_01040"
     CDS             28046..28597
                     /gene="atpF"
                     /locus_tag="VE646_01040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HZD78614.1"
                     /translation="MLVLVAKEQPNPIIPDIAELIYGSVAFIIVFALLAKFAFPALNT
                     MLQERTSKIQGDLEKAEQARTQAESELAEYRRQLADAREEANRIIAEAHRTADQLRRD
                     MQGKAEQEASMTIERAQEEIRAERDRALQDLRAQVGAIAVDLAGRVVEESLDQAAHQR
                     LIDEYIEQVAASNGGNGKGAGGR"
     gene            28587..29132
                     /gene="atpH"
                     /locus_tag="VE646_01045"
     CDS             28587..29132
                     /gene="atpH"
                     /locus_tag="VE646_01045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HZD78615.1"
                     /translation="MGAERSSLIQEYARALLTIASAEGELGLVEDQLYALSKAIDAEP
                     GLRDALADPDLPAENKKALLRDIMGEAANPVAVNALAFLVEQGRGRDVGEIAEAFARA
                     AAESRQHVLAEVRSAVPMDEPQRKRLGQALSRATGKTVEVKVVVDPTVVGGIVARVGD
                     EVFDGTVRNRLQEARRQLAGA"
     gene            29160..30689
                     /gene="atpA"
                     /locus_tag="VE646_01050"
                     /pseudo
     CDS             29160..30689
                     /gene="atpA"
                     /locus_tag="VE646_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854231.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
BASE COUNT         4656 a        10388 c        11313 g         4583 t
ORIGIN      
        1 cggccgactg cgcgtacctc gtggacaagt tctcccgagg cttcgaccac ctgatctacg
       61 tgtggggcgc cgaccaccac ggggacgtgg tgcgcgtgag gggggcggcg gaggcgctcg
      121 ggttcgaccc cgacgcggtc gagatgatcc tgtaccagtg ggtggccctg taccgggggg
      181 gccggacggt ggggatgagc aagcgcgccg gcacgctggt cacgctcgac gagctgctcg
      241 acgaggtggg ggcggacgcc acccggttca cgctcctgtc ccggtcgaac gatgcgacga
      301 tcgacttcga catcgaggcc gtgaagcagc ggtcgatgga gaaccccgtc tactacgtgc
      361 agtacgggca tgcgcggatc gccagcatcc tgcggcacgc ggcccaacgc ggcctggacc
      421 tgctcccggt ggaggaggcc gatctgtccc agttgaggac cgagagcgag ctggacctgc
      481 tccgggccgt ggccgagatg ccggagcagg tggcccaggc cgccaccctg cgggccccgc
      541 accgcctgac ccactacgct caggcgctgg ccgcgcagtt ccatcgcttc tacacggagt
      601 gtcgggtggt gaccgaggat cgggccttga cccaggcccg gctgtggctc tgcaccggca
      661 ccaagcagac catcgcgaac gtcctcgggc tgctcggcgt ctcggccccc gagtccatgg
      721 aacggaccga tgcgtgacgc gctggaccgg ccctcggcac cgggcgatcc ctggccgtcg
      781 gccgcgacct tcgggccgga ggggctccgg atcgccggcg cccgggcgct cgagctggcg
      841 gagcggtacg gcaccccgct cctggtcgtg gacgaggagc acctgcgcgc ccgatgccgg
      901 gccttcgcgg agctgttccc gcatccgctc tacgcggtca aggcgttcac gtcgcgtggg
      961 gcgatccgga tcgccgctga ggaaggcctc gacctgctgg ccgccacccg gggtgagctc
     1021 gaagcgtgcc tgcgcgccgg cgtcccggcg gagcgaatcg tgctgcacgg caacaacaag
     1081 tccgagcgcg agctccacct ggcggtcgac gaggggatcc gcctggtcaa cgtcgacaac
     1141 cgggacgagc tccagcgcct gtccgcgatc gccgaggccg ccggggtgga gcagccgatc
     1201 ctgatccggg tcgtccccga gatcgtggcc ggcgggcacg agaagatccg caccggtcac
     1261 cgcgggtcga agttcggtct cccgctggag gaggtcccgg atgccgtgtc cgaggcggtg
     1321 gccctccccg gcgtgcgccc gctgggcatc cacgcgcacg tcggttctca ggtcctcgag
     1381 ccgggtccct tcctggaggc ggtggacgtc ctgctcgatc tcctggtgcg ggtgcgcgag
     1441 gtatccgggt gcgaggcgga ggtcctcgac ctgggtggag gattcggggt ggcctacacg
     1501 gatgagtcgc caccatcgtt ggagacgctc gctccggcgc tgctcggccg ggtgcgggag
     1561 ggcgctcggt cccggggcct ggcggtcccg cacgtcttgg tcgagccggg ccgagcggtg
     1621 gtgggcgggg ccatggtcac cctgtaccgg gtgggcgccg tgaagcgggc cggtgagcgg
     1681 ctcctcgtgg cggtggacgg cgggatgtcg gacaacatcc gccccatgct gtatggcgct
     1741 cggtacacgg tggcaccggc cggcaccgaa cgcccgggcg ggccgacgac ggtggacgtg
     1801 gtgggcaagc actgcgagtc cggcgacctg ttggctcggg acgtgcggct cccgtggccg
     1861 ccgcagcggg acgacctgtt ggccttcgcg gccaccggcg cgtacacgta ctcgatggcc
     1921 agcaactaca accgggtcgg ccggcccgcg gtggtggccg tccggcgggg tgaggtccgg
     1981 ccgtggctgc gccgggagga cccggccgac ctcgatcggt tggaggtggc cctggaggac
     2041 cggccgatgc ccgagccgcc ggccgaggtc gaggtccgcc cggcccgccc cgcggacacg
     2101 gactcgttcc tggatgccta tcggagcgtg gccgcggagc gtcggttcat ccagaccgag
     2161 gtcgtcaccg gaaccccccg ccagtaccga cggacgttcc gccgcgcctg gacctccgag
     2221 gaggccaccc tgatggcgct cgcggccggc cgcgtcgtgg ggagcctgtc gatccggcgc
     2281 gacccgcagg gcgccacccg ccacgtggcg accctgggca tgttcgtcct ggacggatgg
     2341 cgtggccggg gcgtgggctc ggcgctcatg gccgaggcgc tccggtgggc gcgggcgcag
     2401 ggggtccgac gcgtggagct cacggtctat ccgcacaacc gggccgccct ggccctgtac
     2461 cggcgcttcg gcttcgtgga ggaaggtagg ctcatccggc acgcgaggaa gtcgtacggg
     2521 tacgaggacg agatcctgat ggcggtgtgg ctgggctcgg agggggcgtg atcgaccgga
     2581 ggctgcgcgt gggggtgctc ggtgcgggca ccgtgggggc ggcggtgatc cgtctgctcg
     2641 agcgacaccg cggcgacatc acgcggcgcg cgggctgcga gctggaggtg gcctcggtgg
     2701 ccgtgcgcga cccggccaag gctcgggacg tcccgctcga cccgccccga tccaccaccg
     2761 atccgatgga ggtggtcgac gatccgagca tcgatatcgt ctgcgagctc atcgggggca
     2821 cggagccggc cgggtcgctg atcctggcgg cgctcgagcg cggcaagccg gtggtcaccg
     2881 cgaacaagga gctgctcgcc aaccggggcc gtgagatcct cgacgctgcc gacgccaagg
     2941 ggttggatct gttcttcgag gccgccgtgg ggggagggat ccctctgatc cggccgctca
     3001 aggagtcgct caccggcgag cggctgtccc gggtgatggg catcgtgaac ggcaccacga
     3061 acttcatcct gtcccggatg gccgaggagg atcgaccgtt cgacgaggtc ctgaccgagg
     3121 cccagcgcct ggggtacgcg gaggcggacc ccacggcgga cgtggagggc ttcgatgccg
     3181 cggcgaagtg cgcgatcttg gcctccatcg ccttcaacgc ccgggtggtg gccggagacg
     3241 tgtaccggga ggggatcgcc cgggtcacga acgaggacat cggatacgcg cgacgattgg
     3301 ggtacgtcgt gaagctgctc gcgatcgcgg agttcgacgg cgagcggatc gccgcccgcg
     3361 tccatccggc gatgatccct gcctcgcatc ccctggccgc cgtccgggac gccttcaacg
     3421 cggtgttcgt ggaggggccg aacgtcgggg agctgatgtt ctacgggcgc ggggccggcg
     3481 gggagcccac ggccacggcc gtggtgggtg acctggtggc ggcggcccgg aacctccaga
     3541 acggcacgcg cggggtgggg tgcacctgct tctgggagcg gacgatccgg cccatggaag
     3601 agctcgcgga ccagtactac atcctgctgc gggtgcagga ccgcccgggc gtgttggccg
     3661 agatctcccg cgtgttcggc gacaacgacg tgtcgatccg gagcgtgtgg caggaggggg
     3721 gcggggccga cgccaagctc gtcttcgtca cgcaccgggc actcgagggg aacttccagc
     3781 acgcggtcgc cgccctggac gacctgcccg cggtggaaga ggtgcgcagc gtggtccggg
     3841 tggaggcgga ggagtgagcg ctcggccctg gcgaggcgtg atcgaagagt accgcgaccg
     3901 gctgcccgtc tcggacgcca caccgctggt cagcctgggg gaaggcggga ccccgttggt
     3961 ccgctcggac gccctctcgt ccgagaccgg gtgcgaggtg tacctgaagt acgagggggc
     4021 caaccccacc ggttcgttca aggatcgggg gatgaccctg gccatctcca aggccctgga
     4081 ggagggttcg aaggcggtgg tgtgcgcctc gacgggcaac accagcgcgt cggcggccgc
     4141 ctacgcggcg aaggccggcc tgaccagcgc ggtgctggtg cccaagggga aggtggcgct
     4201 ggggaagatg gcgcagacgc tgatccacgg ggctcgggtc ctggaggtcg atgggaactt
     4261 cgacgcatcg ctagacctgg ctcgggacct gtcccggcac tacccggtca cgctggtgaa
     4321 ctccgtgaac ccgtaccgga tccagggcca gaagagctgc gcgttcgaga tctgcgacgc
     4381 gctgggcgga gcgcccgacc tccactgcgt gccggtgggc aacgccggga acatcaccag
     4441 ccactggatg ggctaccgcg agtacctggc cgatgggctc gtggcggagc caccccagct
     4501 gttcggtttc caggccaagg gcgcggcccc catcgtccgg ggcgagccgg tgcccgatcc
     4561 ctccaccatc gccaccgcga tccggatcgg gaaccccgcc tcctgggagc aggcggtggc
     4621 ggcggcgcgt gagtcggagg gcgccatcgc ggcggtcacg gatcgacaga tcctggcggc
     4681 gtaccggcgg ctggcgcgcg agggcctgtt cgtggagctc gcttcggcgg cgtcggtggc
     4741 gggcctgcta cagcgctcct cggaccgcca gctgccccgc ggcgcgacgg tggtgtgcgt
     4801 gctcaccggt cacggcctga aggatcccga gtgggccatc accggggccg gccacccacc
     4861 caccgtgccg gtcgatcccg ccgcggtggc ggtcgagctg gggttgtagc agcgtgcggc
     4921 tgcgcgcggt ggtcccggcc acctcggcca acctcggtcc ggggttcgac tgcttcgggc
     4981 tggccctgga cctctgcaac gaggtgacgc tggacaccga ggcacggccc ggcgtgcgct
     5041 gggacggcga gggcgcccag gagctcccca ccgacgggac cgacgtggtg agccgggcca
     5101 tcgcggcggc cgtggagcgc cgacgccgac accaccccga gatctcggcg ccccccttcg
     5161 ggctctacgg cgtgaaccgg atcccgctgg agcggggcct gggctcctcg tccgctgccg
     5221 cggtagcggg cacggcgctg gccgccgcgc tcctcggtcc ggtcggctgg gaggaccttc
     5281 ccaccacctt cgcgtacgcc gcggagctcg agggacaccc cgacaacgct gcccccgcgg
     5341 cgttcggtgg cctgacggtg atcgcggacg gcggcgtgca ccggctggac gtgcatcggg
     5401 cgatccggcc ggtcgtgctg gtcccgcaag acgtccggac ctccaccacg cgggccaggc
     5461 gcgaactgcc ggcccgcgtt ctcctggccg atgcgatcta caacgtcgcc cacggcgccc
     5521 tggcggtgag ggccctcacc tccggcgatc tcgatctgct gctggtgtcc ctgcgggacc
     5581 gcctgcacga gcggatccgc ctgtcctcgg ttcccgcggt cgaggagacc ctcgaccggc
     5641 tccgccgctc gcaggtcccc gcctgcgtct cgggatcggg tccatcgctg ctggcgttcg
     5701 aaggggacgg tcgtcctgtc ccgcatccgg gcccagggtg gcaggtgctg ccactcgccg
     5761 tccgagccac gggggcggag gtgatccgcg aaggctaggg ccggacgatc accggcgtgc
     5821 ccggccggag caggggacgg agccgggcca gggcctgctc ggacacgcgg aggcatcccg
     5881 cgctggcgga cgtgccgatg gtggacggcg cgctcgtccc gtggatcgcc agctggtctc
     5941 cgccccacca ccccgccggc aggtgcgact ggatccccga gatcccgaag gcgaagctcc
     6001 cgtacgcact gcccggggag aagggcacgc ggtcggtgac gaagtagcgc cccgtcgggg
     6061 tgggcgtgga tggagcgccg gtggcggcgg ggaagtgggc gacggggcgc gctccgcgca
     6121 tcacggtcag cagatggcga gacagatcca cctgcacgct gatccgggtc ccggtgcgcc
     6181 gcatgccccg gagggcgatc caacccgagc ggagtccggc gctgtaggga accgccagca
     6241 acccgtatcg gccgtccgcc gaggtgcgcc gaacccacgc gacgatcggc gcgtggtagt
     6301 agcgggagac ggccgggacc cagccgatca agcggccggc gccgggctcg gagcggatcg
     6361 gcgtccgtgc ggtcaccctg acgagcagcg cgctccccgg ctgcgaacgg tgcgacgatc
     6421 ggtcggtggc agcggcgcgc tcggcgagcg cccggcccgg ccggagtgcg ggtccggcac
     6481 cccgcgtgat ccccgccgtg gaggccaccg gccgggcgct gctctcgagg gaaccccagg
     6541 tcagcgggac ggctgccatc gcggccacga ggatcagggc acggatcctg ctcgctgcgc
     6601 gggtggtccg tcccatgcct ctgccatcgg cggtccggcc ggcacgctga aggacggggc
     6661 ggatgccgcg ccggtgccgt gttcggggcg ggaaccggga ggttcagccc acgcaggcgc
     6721 cggtggagac ggcgcgggtg gaaccgatcg tgtgggcgag gccggacgag gcggccgtgg
     6781 acgagatcgc gtagccgatg tcacggtcgg tggtcgaggc cgcgaacacg atgccggcca
     6841 cggtcccgtc cgtgaggacg aagggccccc cggagtcccc cggttgcacg gtcgtctgga
     6901 gctcgtagac gctccgctcg accacctgcc gaccgtagat gtcccggccc acggcatcga
     6961 tcgaccggcg aaccgcggct ccgctgccgg tcaggctgcc tccgccggga tagcccagca
     7021 ccgcaccccg cgcgccccgg acggccgggc tcccgctgag cgggagtggc gggcccggat
     7081 cgtgcgccac gtacaggacg gcgaggtcga cgcgcgggtc gaacatcacg gtggtggccg
     7141 actggctcgc gcctccctgc gcctggaccc tgggtgccat cacgccggcc acgacgtgtg
     7201 cgttcgtcat cacgtagtga tcggccacca cgaacccgct gccggactgg atctcgccgc
     7261 aggcctgccc gacgatccgg agggtgctgc ccgaggcggc ggcgaagacg cgccgggcct
     7321 gggccctgga tggcgggtcc accgagccgg ccggcgccgg ggggagcccc aggaacacgt
     7381 cggggaagcc gtaccggttg aaaaacctcc ggatctcgcc gagcagcgac ggcggccggg
     7441 gcaacgaggc atcgaggccc cggaccaccg ccgagcctcg gatctgggag gcgacggcgg
     7501 ggaagggtcc gttcacgagg ttgagcgcga cgaaccagat cgcgagcaga gccgcgccca
     7561 tcgagacgag cacccctccc cccgcatcca ccggccgcaa gccgctgcgc ctggcgcgag
     7621 cccgtaccca ggagccgagg gcccacccca gcgcgttccc gatgctgccc aggccgagca
     7681 gcacgaccac cgcgatcccg gcccggatgg cgtcccctcg ggccagggag gcgagcgggg
     7741 gacccagcag ggctccggcc agcagcccta gggccaggcc gccgaaggac agcacctgca
     7801 ggagagcccc tcgccgatac cccaggtacg cggccaggcc gatcagcgcg atgaggagca
     7861 cgtccagggc atccatgctc ggcgctatcg tctcaccccc cacggcgacg aaccatcgat
     7921 ccgcgatgag gtcctgctgc cacgccgctg cggcgcaccg gtgcctgggc ggactccggg
     7981 accccgctag actggtcgcg tggtcacgat cgcccacatc tccgatcccc acgtgggctc
     8041 cccgtacttc gtccccaacc tgatgaaccg cgtgatcgtc gagctgaacg agctcgcgcc
     8101 cgacgtcgtg atctgcagcg gcgatctgac caacgaaggg tatcggcagg agtacaagaa
     8161 ctgggtggcc tacgccgagc ggatccgggc cccgctccac accgtgcccg ggaaccacga
     8221 cgcccgcaac gtcgggtacc tgcacttcga ggagctgatc gggccccgac attggtcgat
     8281 cgatctgcag ggggtccggg tggtgggagc cgactccagc gagcccgacc tgaacgaggg
     8341 tcaggtgggg cgtgagcgct acggctggat ccgcgagcag ttcgccgttc ccgcggacct
     8401 caaggtgttc gtcctgcacc accacctgct gccggtcccc gggaccggcc gggagcggag
     8461 cacggtgatg gacgccggcg acctgctcga ggaactgatc ggcgcgggcg tgaacatcgt
     8521 cctgtccggg cacaagcacg tcccgtacgt gtggcgtttg gagaacctct acatcgccaa
     8581 cgcggggacc gtggcctccc tgcgtctccg cgggtacacg aaaccgtgct acaacgtgct
     8641 ccggttcgag ggggacgacg tcaagatcac ccgtcggttc cccttcgggg gagagagcat
     8701 catcgcgcat ttctcgctga ccagcggtga gcagttctac cgggagttcg aaccgccggt
     8761 ccagcagacc gttgcgcaga cgggggagta gccggaagtc gtctcgccgg ggggcatcgt
     8821 gagggttctc gcgctcgtcg acggggagca ctatccgccg gtcacccgct gggggatccg
     8881 gcgggccgcc gagcggggac tgcgggtcga gctcgcgttg ttcctgggcg ggaccgagaa
     8941 ggtgctgccg ggcgcgctcc cggacctcgg ggtgccggtg ctgccggccg gcgccgacgc
     9001 cgcgggggcg ctggcctcgg ccctcggcca gcacgccgtg gaggccgtgc tggacctctc
     9061 cgacgagccg gtggtgggct accgggagcg catggagctc gcggccgcct cgctggccgc
     9121 cgggatcccc tacctgggtt cggatttccg gctggatcct ccgatccacg gtccgcctct
     9181 cagcatcccg accgtcgcgg tgatcgggac gggcaagcgg accgggaaga cggcgctggg
     9241 gggggaggtc gctcgggtgg ccgcgcggga agggctgaac ccggtcctgg tggcgatggg
     9301 gcggggagga ccgccggacc cgcaggtggc gccggcgggg accgtcgacc tcgaccacct
     9361 gctggagctg gtccgtcgtg gggagcacgc cgcgtccgac tacctggagg atgccgtgac
     9421 ctccggggtg accacgatcg gcgcccgccg agccggaggc gggttggccg gagccccgtt
     9481 cgcgtcgaac gtccgggagg cggccgagat ggcggtggag ctcggagcag gggtcgcggt
     9541 cctggagggg agcggctcgg ccatcccgcc gatcccgtgg gacgcggggg tgctggtggt
     9601 cccggccttc acgcccctcg agtacgtcac cggctacctg ggtccctatc ggctgttgct
     9661 atcggacctg gttgtcctta caatggctca gagctcacga gcggggctcg agaaccttca
     9721 cgctcttcga tcccacgtcc agcgatcccg tggcgcacca cggttcgtgg ttacggagtt
     9781 cgagccggtg ccgctgggtg acgtgggggg catggatgtg ttcttcacca ccacggcccc
     9841 cgagccggtg gccgcgaggc aggtcgaggt gctcgaggcc ggttacggat gccgggtggt
     9901 gggatggagc gctcgtctgg ccgatcgagc cgggctcgcg caggacatgg acgaggcgga
     9961 gggataccag gttctcctga cggagctcaa ggccgcggcc gtcgacatcg catgcgaacg
    10021 ggcgttggcc tcgggagccg gcgtggtgtt cgtggacaac cgtgccgtcg ccgtggaggg
    10081 agagtccgat cttcccaccc tgctccggga gacgatcgaa ctcgcgagga accgcagagc
    10141 agcgagatga ccccacgatg accaaggatc tgaagagccc gcacatcctg atcagcgacc
    10201 gggagatggg gctgcccttc tccaagggcc tgctggcatc acaggtgatg gtgacgggcc
    10261 tgtcccccta ccgcgcctac gaggtcgcgg aggccgtgga ggatcggctg ctcgacctgt
    10321 cgaggccttc ggtcaccacc gaagagctcg atggggtggc gatcgaggtg atccaggaga
    10381 tcgccggcga acgctacgcg aagaacttcc tgcggtggag ggaggtcgac gcgctggaca
    10441 tcccgctggt gctgctgatc ggcgggacca cgggggtggg caagtccacc atcgccacgc
    10501 agctcgccgc ccgcctgggg atcgtccgca ccgtggccac ggacgcggtg cgcgaggtca
    10561 tgcgggccat gttctcgcac gagctcatgc ccacgctgta ccggtcctcc ttcgaggcgg
    10621 gagacgccct gcgcgagccc cccacccgcg acgcggtggt ggtgggcttc cgcgagcagg
    10681 tgtccgcggt ggcggtgggg gtcgacgcgt tggtggagcg ggccgccctg gagggcatga
    10741 gcatgatcat cgagggggcg cacctcgtgc cgggcttcat ggacctggag tccaggaacg
    10801 agcgcatcct ggcggtcccc gtggtggtga ccgtggagga cgaggaggtg catcggagcc
    10861 acttcatggc ccgctcctcg gaccaccaga cgcgtccggc cacgcgatac ctgagaggct
    10921 tcgacaacat ccggcgcgtc cagcgctaca tcaagagcca agccctgtcc cacggcgtgc
    10981 cggtgatccc caactacaac ttcgatcaca ccctggcggc ggtgatcgac ctggtgatgg
    11041 agcgggtaac ggaacgcgcg tccgtgctgg ctccgacggc gaacgggacg aaaggacgcg
    11101 gtgcatgagg ctcttcctgg atacggccaa cctcgaggag atccgagaga tcaaccggtg
    11161 gggggtgctt tccggcgtga ccaccaaccc cacgctggtg ggccgggaga acgaggatcc
    11221 cggccagctc tggaagcaga tcctggccga ggtcgacggc gacgtgtcgc tcgagaccac
    11281 ggagccggaa gccacgccga tgtacgagca aggcgtgcgg ctcgccggca tgggccccaa
    11341 cgcggtggtc aaggtcccca tgaccccgtc cggcctggag gccggcaagc ggctagtggc
    11401 cgacgggatc aagatcaacg tgaccctggt cttctcgccg gcgcaggcca tcctggcggc
    11461 cgagatcggg gcctacatcg cctccccctt cctcggtcgc atcgacgacg cggcatcgga
    11521 cgggatgcat gcactggccc agatctgcga tatctacgcc gtgcagggct acgacacgca
    11581 ggtcctggcc gcctcgctcc gccacccgat gcacgtggtc caggccgcgg agctgggagc
    11641 cgatatcgcc accatgccct acgacgtgtt caccaagctc gtgaagcacc cgctcacggc
    11701 tgccgggctg cagcgcttcg acgaagactg gcagacgctc cagcggggat tgaagggagg
    11761 agcctagttg gcaccggaga gaaccatgga caggggcgac cttgaaggca aggtcgcatc
    11821 cgagctccac acgatcgccg atggcctcgg gatcgagggg caccagcgcc tgaagaaggc
    11881 cgatctgatc gagcggatcc tggcgcactc ggggggcggc gggaacggag cgcaggcggc
    11941 cgccggcgct gccgcggaga cggctccccc cgccgagacg tcgtcgccga acggcgaggt
    12001 ggcgtccgac cggaccacgg tccaggagtc cccgtcccag acgtcggagg cgccccccgg
    12061 ggtccgggag gagccgcggg ccgaggaccg gtcaccgcgc ccacggatcg agcgggacac
    12121 gcgggaggac ggcgggggcc gtcccgagcg cgaggaccgc ggcgaacgtc ccaaggggcg
    12181 agaaccgcag ggcgaaccga ggagggatcg acccgagggt cgcgggggcg agggccggca
    12241 ggacacccgg ggcggcgagg gcggacgtcg acgcctgtcc cgcgaggagc gccgccgcca
    12301 gcgcgaggag cgccgacagc gggagatgga ggcgcgcgag gaagagatcg ccaatgcccc
    12361 cacggccacc gggctgctgg acatcctgcc cgagggttac gggttcctgc ggacgtcggg
    12421 ttacctgccc ggctccgagg acatctacgt gtcgctgtcg caggtacggc ggttcatgct
    12481 ccgcaagggc gacaccgtca ccggcaagat ccgccagccc aaggacaacg agaagtactt
    12541 cgctctgctg cagatcgagc acgtgaacgg gctcgagccg gaagaggcca agcagcgcgc
    12601 gcatttcgag aagctgacgc cgctgttccc gcacgatcgg ttcaggctgg agacggcgcc
    12661 cggcgacgtg accggtcgga tcatcgacat ggtggcgccc atcgggcagg ggcagcgcgg
    12721 catggtggtc tcgccgccca aggcggggaa gaccacggtg ctgaagaaca tcgcgaacgg
    12781 gatcatcgcc tccagccccg acacccacgt gatcgtgctg ctcgtcgacg agcggcccga
    12841 ggaggtcacg gactggcagc gcacggtgga agcggcggag gtcgtgtact ccaccttcga
    12901 caaacccgcc gatcagcaca tccaggtcac cgagctggtg ctggagcgcg ccaagcgcct
    12961 ggccgagacc ggcaagaacg tggccatcct gctggactcg atcacccgtc tggcccgcgc
    13021 gtacaacctg gcggcgccgg cgtcgggcaa gatcctgtcc ggaggcgtgg actccaccgc
    13081 cctgtacccg ccgaagcggt tcttcggtgc ggcccggaac atcgaggagg gcggcagcct
    13141 caccatcatc gcgagcgcgc tggtggagac cggatcgcgg atggacgagg tgatcttcga
    13201 ggagttcaag ggcaccggca acatggagct gcggctggac cgggcactgg ccgagaagcg
    13261 gatcttcccg gccatcaacg tcgaggcctc gggcacccgc aaggaggagc tgttgatgcc
    13321 caccgaggag ctcgcgttgg tgtggcggct gcgccgggtg ctgcacgcgc tcgagccggg
    13381 tgccgcgctg gagctgttgg tggacaagat ccgcgccacg aagtccaacg agcagttcct
    13441 gaaggagatc gccaaggcgc cgtcgtgatg cgaacggccg gatggaggag agacccgaac
    13501 cgatgaagca ggacatccac cccgagtaca tgatcgctac cgtgcactgc tcgtgtggga
    13561 acaccttcca gacccggagc accaagcccg agctccgggt ggagatctgc tcgaactgcc
    13621 atcccttcta cacgggcaag cagaagctcg tggacaccgg cgggcgggtc gagcggtttc
    13681 agcggcgcta cgggaagcag gcgccgaccg gtgggtccgc acaggcgcgg cagccgcagg
    13741 gccagggctg acctcgagca tggccgcgga ggcgccggcc aagcagcact tctacggcgg
    13801 ccaggccgtt atggagggcg tgatgatgcg cggccgggac gtgtgggcga tcgcggtccg
    13861 gcgctccgac caacggctgc acgtggagtc gcacgacatc cactcgatcg ccgagcgcca
    13921 tccgatcttc tccaagccgg ggctgcgggg cgtcatcgcc ctgggacagg cgctgtcgat
    13981 cggcgtccgg gcgctggcca tctcggccaa ccaggcctcg cccgaggagg agcagctctc
    14041 ctcgcgccag atggtcctct ccatggtggt cgcgttcgcg atcttcgtcg ccgtgttcat
    14101 cgtgggaccg gcggcggcgt tccgctgggt cggggggcac ctgcgctcca gcatcctggt
    14161 gaacgccctg gagggcgtct tccgggtgct gctgttcctg ggctacctac tcgccatcgg
    14221 acggaccagc gagatccgcc gggtcttcga gtaccacggc gccgagcaca agacgatcgc
    14281 cgcctacgag cacgacgagg agctggtacc cgagcgcgtg gatcgctact ccaccctgca
    14341 cgtgcgctgc ggcacgaact tcctgctgat cgtgatggtc ctgacgatcc tggtgttcgc
    14401 gttcttcggc gatccgggga tctggtggcg gatcggctcg cggatcgtcg ccatcccgat
    14461 gatcgcgggg ctggccttcg agatgctccg cctcggcgcg aagttcgagc gatccacgct
    14521 gatgcgcgcc ctgatggcgc cgggcctgtg gctgcagaag atcaccactc ggccgcccga
    14581 tcacgggcag atcgaggtgg ccgtcgcatc cttcaacgag gtgttgcggc gtgaggccga
    14641 gaaggtggcg atgcgggcgg gcctgcaggt ctgattcgtc gggtcgttga ttcccggccg
    14701 ccggcccgta gcatgaggca agcggctccc caaccgaccc gcaggaggcg atccatgtcc
    14761 tcatcggagc tcgaccttcc cgtgctcgcg agccccgacc agatccggcg ccgcgagttc
    14821 gtcaccacga ggcggggcta cgatcccgag caggtccgcg actacctcga gcagctcgcc
    14881 gcccagatgg agcggctcgg cacgatggcc cgtgaggcac ggatgcaggc ggacgcggcg
    14941 caacgcgcgc aggccgagac cccgcaggcc gatccctacg aggaactcgg acgcaggatg
    15001 tcgaacctgt tccgcaccgc tgagcaggag gcggagcgga tcggccgcga gtcccgcgcg
    15061 gaggcggagg agatcatccg cgaggccagg gccgaggccg gacggatccg gctcgacgcc
    15121 caagccaggg cggaggaggc tcgcgacgcc gcggatcgcg ccctgcgtga ggcgcgggag
    15181 cgcgccgacc ggaccctcgc cggtctgacc acccggcgcg atgcgctggt gcagcagctg
    15241 gccgccatgc aggagcggct cctaggggtg gcccgcgagc tcgaggtggc gatcgatcgc
    15301 gacgagaccc acacggtcgt gctgggggaa gcgggcccgg cggaggagga gcgctccgag
    15361 cacgccaatg tccgtgccgg cggcacggag gacaggagcg ggtccgccgc cggaacggca
    15421 ccgggcggct tcgaagagct gtggtcaaga tccgagggcg acgccgacct gtcgatcccg
    15481 gacattccgc ccctggatct gggctgggac gaggacgatc cgggctcgtc gtagcccacc
    15541 gtgcgtcccc ggtcgcggcc cgtttgggat gatcgaggtc gatggacctc accgaacgcc
    15601 tggacgagct cgagcatcgg tacgaggggc tgtccggtcg gatggcctcc cgcgaggtgg
    15661 cctccgatcc cgagctgctg cggagcctgg gcaaggacct ggcggagctg caggaggtgg
    15721 tcctgcccta ccgcgaatac aaggccgccc tgcatcaggc ggacgaggct cggatgctcg
    15781 gctctcggga accggaccag gagatggccg ccttcctgca ggaggaggag gcggcggccc
    15841 gcgggcgggc gaccgagctc cggcgacgcc tggaggagct cttggtgccg aaggatccca
    15901 acgacgacaa ggacgtcgtg ctggagatcc gcgcgggggc gggcggacag gaggcggctc
    15961 tgtgggcggg tgagctcttc gagctgtacc gccattacgc ggagcgccgg cgctggaaga
    16021 ccgaggtcct ctcggcctcg ccctcggacc tcggcgggtt caaggaggtc gtgctcgagg
    16081 tccggggccg gggcgcgtac tcacgactga agcacgagtc gggggtgcac cgggtccagc
    16141 gggtcccggt caccgagtcc tccggccgga tccacacgtc caccgcgacg gtggccgtgc
    16201 tgccggccgc cgaggacgtg gacgtccaga tccggccgga ggacctgaag accgacgtct
    16261 accgctccgc cggtccagga gggcagtcgg tgaacaccac ggactccgcc gtgcggatca
    16321 cccacctgcc cacgggcatc gtcgtggccg tgcaggagga gcgctcgcag ctgcagaacc
    16381 gtgagaaggc catgcggtac ctgcgcgccc gactgctcca gcaggcccag caggaggccc
    16441 aggccgaaga ggccgaggcg cggcgcgctc aggtgggcac cggcgagcgg gccgagaaga
    16501 tccgcacgta caacttcccc gagaaccggg tgaccgacca ccgcgtcaag atcacgctgc
    16561 accggctgcc ggacgtgctg caaggggaca tcgacgggtt cgtcgatgcc ctgatggccg
    16621 ccgagcgcgc ccgtcagctc tccggcgacg gtagggggtg atggcgatgc gacccgccct
    16681 ggtgctgcgc cgcgccaccg actacctgga gcgccacggc gtggagagcc cggagcggac
    16741 cgccgaggtg ctgctggcat cggtcctggg gaccgaccgg gccggtttgt acgcgcggag
    16801 cacggggctg tccaccgccg aagcgcggat gttcggacgg gcgctctgcc agcgttgctc
    16861 cggcacccct ttgcagcacc tgaccgggga ccaatccttc cgcggcatcg tgctccgggt
    16921 gcgtccgggg gtcttcgtcc cgcggcccga gaccgagatc ctcgtggagg tggccctgac
    16981 cgagctggac cggcaggaag acccggtggt ggtggacgtc ggaacgggga cgggagcgat
    17041 cgccctggcc atcaagcagg agcgacccgg ggctgccgtg tacgcgacgg acgcctcgat
    17101 ggaggcggtg cggctggcca ccgagaacgc cagccgactc gggctggaca tcaccgtggt
    17161 ggaaggggac ctgctggagc cgctcgccgt cgaactccgg ggctggatcg atctggtggt
    17221 cagcaacccg ccgtacgtga cggccgagga gtacgagacg ctcccgcccg aggtgcgcgc
    17281 cgatccctcc tcggccctgt tgggcgggac cgccgtgcac gaacggctgg ttcaggaagc
    17341 gctccgctgg ctccgggacg acggcgtcct ggccgtggag atcggcgccg cgcagggcga
    17401 ggccgtctgg cgagtgttcg ccgcgtccta cacctcggtg cgggtggccc gggacctggc
    17461 gggtcgcgat cgggtggtgg tgggccgacg accgtgaggg atcgggtgga ggcggcggcc
    17521 gctgccgcgc tgtcggggga gctggtggtg ctgcccaccg acaccgtgta cgggatcggg
    17581 acgcggccgg acgatcccgc tgccaccgag cgcgtgttcc gggccaagca ccggtccccg
    17641 acgctcgagc tgccggtcct ggtgccgagc cccgaggtcg cccgcgggat cgccgtgttc
    17701 gaccgggccg ccgagacgct ggccgcacgg ttctggcccg gcccgctcac cctggtgctt
    17761 ccccggaccg agaggagccg ggggtgggag ctcggggggg atggtcgttc ggtgggcgtt
    17821 cggatgcccg atcacccgct ggccctggcc ctcctgcagc gcaccggccc cctcgcggtc
    17881 accagcgcga atccgagcgg cgaggcgacc ccggtggatc cgggcgggct ggagcgcgtc
    17941 ttcggcagcg ccgtgtccca gtacctgtgc tcggagccgc tcccgcctgc accgccctcc
    18001 accgtggtgg cgatctcagg gaccgacctg cggatgctga ggatcggtgc gctttcggag
    18061 cgggcgatcc gaagcgccct gggggcgacg gccggtccga tgtaggtccg atgctagact
    18121 cgcccggtcc catgccccac gtcctcgtcg tctgtaccgg gaacatctgc cgctcgccga
    18181 tcgccgaggg cttcttgcgc gccgcgttcg agcgccggct ggggccgcag gctcccgcgg
    18241 tcacctcggc cggcctggtg gcccgcgatg ggaacgccgc cgagcccgag gccgtccggg
    18301 cggccgccga acatggggtc gacatctcgg accacgccgc ccggcgcctg accggacgga
    18361 tgatggccga ggccgatctg gtgatctgca tgacgtccgg ccaccgggac cggatcgtgg
    18421 aagcgctgcc cgacgccctg gccaggacct tcacgctgaa ggagctggtc ttcctcctgg
    18481 agtcgctgcc cgatccggcc gcccacgcac cggccgaccg cctgagcgag cgggtggcgg
    18541 cggcccacga gctgcgggag cacagcgacc ggccggtcga cgaggacatc ggcgaccccc
    18601 tgggcctatc gatcccggcc ttccgtgcca cggcgatcga gctggagggg ctgtgcaccc
    18661 gcctcgccga cgggctcttc cccgagcggg aggagcgtcg ggctgctggt cacggaggag
    18721 cgtgaggggt gcggctggcg atcggctccg atcacgccgg cttccccctc aaggggccgc
    18781 tgtgcgagtt cctggaggga gaagggcatg agatcctcga tgtcgggacg aactcggagg
    18841 agccggtgga ctacccggca ttctgtgcgt cggtggcgcg ggccgtggcc ggtggagacg
    18901 tcgatcgcgg gatcatcctg ggtggctccg gacagggcga gcagatcgtc gccaacaagg
    18961 tccacggcgt ccgggccgcc ctgtgtcacg acctctacac ggcgcgcctg tcccgtgagc
    19021 acaacgacgc caacgtgctc acgatgggag cgcgcatcgt ggcggcgacg ctggcccagg
    19081 agatcgtccg cgtgtggctc gacaccccct tcgatggggg tcggcacgag cgccgggtgg
    19141 agcagatctc gatgatcgag cggggagaga tgtgatgggc ggcccggtgg ccgactgggg
    19201 ggcgttggag cggaccgacc cggagatcgc gcgggcgctc actggtgagc tcgaacgcga
    19261 gcggcggacc ctgcggttga tcgcctccga gaactacacg agccgcgcgg tcctcgcggc
    19321 cctcggatcg acaatgaaca acaagtacgc cgagggctac ccggggcgcc gctactacgg
    19381 aggctgccgg ttcgtcgacg tggccgagga gctggccgtg gcgcgcgcca aggagctgtt
    19441 cggcgccgag cacgccaacg tgcagccgca cgccggggcc caggccaacc tggcggtctt
    19501 cggagcgttc ctggagccgt actcggacga caaggtcctc gggctggtgc tggcgcacgg
    19561 cgggcatctg acccatggat cgccggtcaa cgtctcgggc atgtggttcg acttcgtggg
    19621 atacgaggtg gacccgcaga ccgagcagct cgacatggac cggatccggg agctggcgaa
    19681 ggagcaccga ccgaagatcg tgctggccgg ctacacggcg taccctcgga cgatcgactt
    19741 cgccgccttc cggtccatcg cggacgaggt gggtgccctg ttctgggtgg acgcgtccca
    19801 cttcatcggg ctctcggcgg gaggggcgta cccgaacccg gtgccgtacg cggacgtggt
    19861 caccttcacc acgcacaaga ccctccgggg accccgcggc gccgtcatcc tgtgccgcga
    19921 ggaacacggc cgggccatcg accgagccgt gttccccatg atgcagggcg ggccgctcga
    19981 gaacgtgatc gctgcgaagg cggtggccct gaaggaggcc atggagccct cgttcgccga
    20041 gtacacgcac cgcacggtcc gctccgcgcg caccctggcg cagggcctgg ccgaggaagg
    20101 attgcggatc gtctcgggcg ggaccgattc gcacctggcc ttggcggacc tgcggcccct
    20161 gggggtgagc ggccgcgacg ccgaggtggc gtgcgaccag atcggtatcg ccctgaacaa
    20221 gaacgggatc cccttcgatc ccatgccgcc gctgaacccg tcggggatcc gggtgggcac
    20281 accggggccg gccaccctgg ggatggacga ggcggagatg aaggaggtcg cggccgtgat
    20341 cggcaggctg ctgcgacgtc ccgaggacga gagcaccagg gccgcgtgcc gggccagggt
    20401 ccaggagctc tgcgaccgct tccccgtgta cccgatctga tcccgcccca tcggagctgc
    20461 ccaggcgggt accatcacca gcgggttggt tccctatctg gtcgtcttcg gcgccgccgc
    20521 cgtcgcgacg ttcgccacga cgccgctggt gcggtgggta tcgctgcgtg tgggctggat
    20581 cgaccgtccg tccgatcgga aggtgcaccc gaaccccaca ccgaccgcgg ggggactggc
    20641 catcttgctc ggcatcgtga cgggcctggc cgtctcgcgc ctggtcccct tcctgcgcgg
    20701 tctgcacgag acgacgtccg agccggacgc ggcgctactg gccgccctgg ccatcgtcct
    20761 ggtcgggctg atcgacgacg tgaagggtct gtctgccgcc gggaagctcg tgggccaggt
    20821 cctggccgcc ggcatcctgg tgctggccgg cgtccagctg ctgtacttct acctcccgat
    20881 cgggggcatc gggattctgt cgttgtcgcc ggacatggcc gtgcccctca cggtggtctg
    20941 ggtggtggcc accgtgaacg ccgtgaacct ggtggacggc ctcgacgggt tggccgccgg
    21001 catggtggcg atcgcggcca tggccttctt cgcgtacatg taccgagctc cgggcgtgga
    21061 cggagctcca tccaccgccg cgctgctctc ggcgctggtg gcgggggcct cggtcggctt
    21121 ccttccgtgg aacttccatc cggccaggat cttcatgggg gactcggggg ccatgctctt
    21181 ggggctgatg atggccgctg ccagcatctc gggcgtcgga cggaaccagt cgccgaccgg
    21241 cggggacttc gccgccgtgg ccatccccat cgccgtcccc ctgatcatcc tggccgtgcc
    21301 cttcctcgat gtggtgctgg ccatcgtccg acgggtccgt cgcggtgctg gcatcacgca
    21361 cgccgacaag gagcacatcc atcatcggct gctggacatc gggcactcgc accgcaaggc
    21421 cgtgctcctc atgtacctgt ggagcgcgct gatctccgcg tgcgccctgg ccgtggcctt
    21481 catcaacggc cggatgctgg ttgggggcat cgtcgccgtg gccgtgatcc tcgccgcggc
    21541 gatccccagg atcgtcatgc gacgtcccgc gggtgtgccg atccggcggg tgcgaggcga
    21601 tgggcagatc gaagcagggc tctcgctcga ccacggcccg gcgaggacac cgatcccctc
    21661 gccagagcct ccggcagcgc cgcaccaggg ctcctgaacg agggtcacta ggaccgatat
    21721 atgggggacg ggcggcacat cctcaggggt tgtccactcc gtacaagaga ctgaatggga
    21781 gtcagcggaa ccagggctcg cggcgggggg cgaggcacgc gatgatgccc ccgctcccgg
    21841 ggcatacgat cgggccgggg gttgggccga agaggggaga gccgtggcgg agatcgacgt
    21901 tcgcctggac cgcgtgacca aggatttcgg cgtgacggtc gccgtggacg acctgtcgct
    21961 cgacatcagc gagggggagt tcttcagcct gctgggcccg tcggggtgcg ggaagaccac
    22021 caccctgcgg atgatcggag gcttcgagga gcccaccagg ggcacgatct acctgggcgg
    22081 gcgtgacgtc acggacctgc ctccctaccg gcgcgacgtc aacaccgtgt tccagtccta
    22141 tgcgctgttc ccccacctga cggtgttcga gaacgtcgcc ttcggtcttc gcaggcgcaa
    22201 ggtgaacaag cacgagatcg acgaacgcgt gaacgatgcg ctgcgcctgg tcgatctgac
    22261 cggcttcgag aaccgcaagc ccgggcagat gtcgggagga caggcccagc gggtcgccgt
    22321 ggcccgggcc ctcgtcaatc ggcccaaggt cctgctcctg gacgagccgc tgggcgccct
    22381 ggacctgaag ctgcgcaagc ggatgcagct cgagctgaag cggatccagg gtgaggtcgg
    22441 ggtgaccttc atctacgtga cccacgacca ggaagaggcc atgaccatgt ccaaccgggt
    22501 cgccgtcatg cgcggcggcc ggatcgagca ggtggggcct cccgaggacg tgtacgagaa
    22561 cccggtcacc gagttcgtgg ccagcttcct cggcgcgtca aacctgctgg acggcgaggt
    22621 caaggaggaa cggaacggga ccgtggcggt cctgctgacc gggggggaca cggtggcggt
    22681 cccggcggag cgggcgccgt caggcttggg atccaaggtc aaggtgggcg tgcgcccgga
    22741 gaagatcacg atccgggcgg agggagcgga acctcggcag ggctggaacg gcatcacggg
    22801 cttgctccgg atgtcgacgt acatcggcgt gagccatcag tacaaggtgg aggggcccgg
    22861 gggggcggag atcaccgtgt gggtgcagaa cctcggtacg gaaccggtcc cccgtccggg
    22921 ggagcgtgtt cgactctcct ggccgcagga gcacaccttc gccgttctcc cgcaggaggg
    22981 attgaccctg gaggaggagg agggagcatg aaccagcggg acgatcggac gttcctcgag
    23041 ccgcagcgga tgcaggaggt cctgcggcgg agactgaccc gtcggagcct cctcaagggc
    23101 gccgggacgg gcgcggcggc cctcggcctc ggggggctgc tggcggcatg cggcggcggc
    23161 accagcggcg gcggcggcac caacggaggc gggggcagca cctccagcgc cttcgacgtg
    23221 agccagatct acagcgggga ccccggatcc agcatcaact tcgcgaactg gccgctgtac
    23281 atcgaccacg ccaaggacaa gaacggcaac cggtacatcc cgtccctgcg caacttcacc
    23341 aaggagacgg gcatcgacgt cagctatcaa gaggtgatcc aggacaacac cgacttcttc
    23401 ggcaagctgc agccgcagct gcaggccggg gacgacaccg gctgggacat catcgtgatc
    23461 acgaacgggc gcgaattcac cgcgctcacc aagctcgact gggtctaccc cctggatccc
    23521 ggcaagcggc cgaacttcga cgcgaacgcc gccgcctggg ccaagaaccc accctacgac
    23581 ccggacaaca cgcacgggat ggcctggcag tccgggatca ccgggattgg cgtcaacgac
    23641 aagctggtca agggctccgt gaccaagctc gacgacctgg ccaacccgga catcgtgggt
    23701 gtgggcaagg tgggcatgct gaagaacgac atgcaggact tcgtgatgat caacctcggc
    23761 atcgacccca ccacctcggg tcccgacgag tggaaggagg cggcggactg gctccggaag
    23821 cagcgcgata ccggtgtggt gcgccagtat tacgaccagg gctacgtgga cgatctgacc
    23881 gccgggaacc tgtcggccac gatggcttgg tcgggcgacg tcctctacta caagctgtgg
    23941 gccggctacg acaacctgga cttcgtcttc ccggacgggg gcgcgctgct gtggatcgac
    24001 aacatgatga tccccgcgca cgccaacaac ccggtgggcg ccatggagct gatggactac
    24061 tactacaagc ccgaggtcgc caccggcgtg accgaatggg tcctgtacat gtcccccgtg
    24121 ccggccacca gggatctgat catgaaggac tcccaggcgg cgcgggacga ggggtccaag
    24181 gggtacgcga acaagctgga ggccacggcg aacagcccgt acctgtaccc cgacgatgcg
    24241 ttcctggccc gcacgaagtt cgggcgtgac ctgaagaccg acgaggaacg gcaggagtgg
    24301 gactcgatct tcctgccgat cacccaggcc tagcggccat ccgagcggga gagccgggac
    24361 caggtggaca gctcgacggg ggcggcgcaa cagggtcggg tcaggggccg gagcatcggt
    24421 cttcgcaaga agctcgtgcc ctactacctg ctcggaccgg gcgcggtctg gctcctgatc
    24481 ttcctggtcc tgccgctcct tctgctggtg tacacgtcgc tacagagcgg tggtctgctg
    24541 gggggcttcc acttcacctg gcagttctcg aactacgccg acgcgctcag ccagaacggg
    24601 aagtacttcg gccgatcgct gatgtactcg tccatcgtca cggtggtggc gttgctgctg
    24661 gcgttcccca tgacgtattg gatcgccttc tacggtggcc ggtggaaatc ctcgctgctg
    24721 ctgctgatgc tgctgccgtt cttcgtgtcg ttcatcatcc gcacgatcca gtggaagttc
    24781 atcctcgcag acaacggcat catcctgggc ccgctcaaga acctgggact gctgccccag
    24841 agcttccacg tgctggcgac gccgttggcc gtggtggccg ggatcaccta cgacttcctc
    24901 cccttcacgg cgctgcccct ctacgtctcg ctggagcgga tcgacaagtc gctggtggag
    24961 gcggccaagg acctgtacgc gagccgctgg caggcgttcc gccacgtggt ggtgccgctg
    25021 tcgctgcccg gggtgttcgc cgcgttcctg ctcaccttcg tcccggccac cggggactac
    25081 gtgaacgccg ccatcctggg tgggccgggc accacgatgg tgggcaacat catccagacc
    25141 aagttcctgt accagaacgt gtacttcgag gcggccgcgc tctcggccgt gctcatgctg
    25201 gggatgttcg tgctggtcag catctacgcg agggtgctgg gcacggaaga ggtcgtgagg
    25261 gcggcggcgg catgagcgtg gtgaccgcgg agcgcgagca gcgcgtcctg gagccggcgc
    25321 cgctgcaccg gccgaacaag cgctggaccc ggttcctcct gcccggcttc accggtctga
    25381 tgatcgccta cctgatgagc ccggtgttcg tgatgatcct gtacgggttc aacaacatcc
    25441 ccggtgagcg ccagaccccg aagttctggg gcttcacgat ccattggtac aaggagctgt
    25501 tcgcgatcca gggcctgacc acggcgatca agaactcgct catcatcgcc cccacggccg
    25561 ccgtggtggc cacgatcatc ggcaccttcc tggggctcgc cctgggccgg tacctgttcc
    25621 ggggcaaggg cgcggtggac ttcttcatct tcctggcgat cgcggtgccc gagatcgtgc
    25681 tgggctcctc gctgctgtcg atgttcgtcc aggccagggc gtccttgggg ctcggcacga
    25741 tcctgctctc acacatcgcc ttcgacgtgc cgttcgttgc cgtcacggtc caggcgcgtg
    25801 tggcggggat ggaccgatcc ctcgaggacg ccgcccagga cctgttcgcc gatcccgtca
    25861 ccacgttctt caaggtcacc ctgccccaga tcctccccgg gatcttcgcc gggttcctgt
    25921 tggccttcgt cctgtccctg gacgacttcg tcatcaccca gttcgtgtcc ggccagaccg
    25981 agaccttccc gatctgggtg tgggggtcca cgaggatcgg gctgccgccg caggtgaacg
    26041 tgatggcgac gttgctgttc accgccggcg tggtgatctc gatcgccaac gtcgcggcgc
    26101 agcgcctccg ccgctccagg gccgcctgaa gaggaaccga cgcatccagg cctgctggag
    26161 gattcgtgaa gagtttcaca agcgctccgc gcgccgtccc catagtggca tctagctgcg
    26221 aaaacgctcc gccgtcgcct ttgacgggcc cgttgggccc cggatatact gccgcgcgcc
    26281 catggccgga ggacacgggg atgcatggag tggcatgggc acggcgtgga acgtcatggg
    26341 gacgctcgcg tcgggcatcc tcgtctgggg cggcatcggg tacctggtcg atcgggtggt
    26401 ggggttccac tggctcttcc tgccgatcgg gatggtgatc ggcgtctcgg cctccatcta
    26461 cgtggtgtac ctgcggtatg gcagagatca ctgagcgggc ggagcgggag ctggtccgga
    26521 gggctctggt gccctcggct gtggccgttc ccctctcgct gctcatcggg tgggccctgg
    26581 ctggtcccgg tgtcggggtc tcggcggcca tcgggatcgc cctggtcttc gccaacttcg
    26641 cggcccacgg cctctcgctg gcctgggcgt cgagagtatc catcgtcgcc gtgcaggtcg
    26701 tggccctggc cgggttcgtc gtccggctcg gcgtggtcgt ggccgcgatg ttcgtcctga
    26761 acaccctggc gtggttctcg ccgctcgcct tcgggttggc cgtggtcgtc gcaacgctgt
    26821 cgctgttggt ctatgaggcg cggctcgtgc tgcgcggcat gggcgggacc ctcatgatcc
    26881 cggccgatcc ggtggccgcg caggcggccc gagccttggc ggccagggag gcacggtgat
    26941 gctggctgcc ctcgcgtccg ggttcgcccc ggcggtcgcg gcgctctccc gtccacccga
    27001 aggcttccac gccccagaca cggcgatctt cgactactcg aacaactgcc tgttcggcaa
    27061 cggctcgtac tgcatcacgc gcccgacgtt ctggctgttc gtctccgtcc tgatcatcgc
    27121 ggcggtcttc atcgccgcgt tccgccgtcc tcagctggtg cctcgggggt tgcagaacgc
    27181 cgtggagtcc ctcatcgact tcatccgcaa cggcatcgtc atggaggtca tgggtcccga
    27241 tggccttccc tacctcccgc tgctgacgtc cttgttcgtc ttcatctggg tgaacaacac
    27301 gttcgaggtg atcccgttca tccagttccc gcccacctcg aagatcgcga tccccatgat
    27361 cctggccgga ctggtctggt tcatcttcaa catcgtgggc atcgtgaagc acggggggtg
    27421 gcggtacctg aagagcatca tgttcccgcc gggcgtccct ccggcgatct acatcctgta
    27481 cacaccgatc gagctggtct cgacgatcat cgtgcgtccc gtcaccctga ccgttcgtct
    27541 cacggccaac atgatcgccg gtcatctgat cctgacgacg ttcttcctgg ggacctggta
    27601 cctgcagtgg aagctgctca cgatcccctt cgcggcggcc tccttcgccc tggggacgtt
    27661 catcacgggg ttcgagatcc tcgtctccgt cctgcaggcc tacatcttca cgatcctgac
    27721 cgccgtgtac atcgcctcat ccatccaccc agagcattag agaaggggga cacacatgtc
    27781 gcatctgttc ggcctgctcg cccaggtgac cgacacgggc gtgagctcga tcggcaaggg
    27841 gctcgtgtac ggcctcggtg cgatcggccc gggcatcggc atcggctacc tggtcggcaa
    27901 ggccgtcgag gccatggccc gccagcctga ggcagcgggt atggtgcgga ccacgatgtt
    27961 cctggggatc gccttcgtcg aggcgctcgc gttgttcggc ttcgtactgt cgttcatcgt
    28021 ctgatcgggg ggcgggatgt tgcacatgct cgtactggtc gccaaggagc agcccaatcc
    28081 gatcatcccc gacatcgccg agctgatcta tggctcggtg gccttcatca tcgtgttcgc
    28141 gcttctcgcg aagttcgcgt tccccgccct caacacgatg ctgcaggagc gcacgtccaa
    28201 gatccagggc gacctggaga aggcggagca ggcccgcacc caggcggagt ccgagctggc
    28261 cgagtaccgt cggcagttgg ccgatgcacg ggaggaggcg aaccggatca tcgccgaggc
    28321 gcaccgcacg gccgaccagc tccgccgcga tatgcagggc aaggccgagc aggaggcctc
    28381 catgaccatc gagcgcgcgc aggaggagat cagggccgag cgcgaccggg ccctccagga
    28441 cctccgggcg caggttggcg cgatcgccgt ggacctggcc ggcagggtgg tcgaggagtc
    28501 cctggaccag gcggcccacc agcggctgat cgacgagtac atcgagcagg ttgccgcttc
    28561 gaacggcggc aacgggaagg gcgcgggtgg gcgctgagag gtcctcgctg atccaggaat
    28621 acgcgcgggc gctgctcacc atcgccagtg ccgagggcga gctcgggctg gtcgaggacc
    28681 agctgtacgc cctgagcaag gcgatcgatg cggagcccgg actccgtgat gcgctggccg
    28741 acccggacct tccggccgag aacaagaagg cgctccttcg ggacatcatg ggcgaggcgg
    28801 ccaacccggt agccgtgaac gcgctggcgt tcctggtcga acagggccgg gggcgcgatg
    28861 tgggggagat cgccgaggcg ttcgcccgcg ctgccgccga gagccgacag cacgtcctgg
    28921 ccgaggtgcg ctcggccgtc cccatggacg aaccccagcg caagcgtctg ggccaagcgc
    28981 tctcccgggc caccgggaag acggtcgagg tcaaggtcgt ggtggacccc acggtggtgg
    29041 gggggatcgt ggcgagggtg ggggacgagg tcttcgacgg cacggtccgc aatcgcctgc
    29101 aagaggctcg tcggcagctg gccggggcct gagcgtgatg acgaggagca tgtgagccga
    29161 tggcactgaa cctggacccg cagtcgatcg ccgaggcgtt gcgcaggaac gtcgagagct
    29221 ggaccccctc ggtcgagcgc ggcgaggtcg ggcgcgtcgt cgaggcccac gacggcatcg
    29281 cccgcgtgaa gggactgcct cgcaccatgg ccaacgagct cctcgagttc cccggcggcg
    29341 tgatgggcat cgccttcaac ctcgaggagg accagatcgg cagcatcatc ctgggtgagt
    29401 acgagcacat cgaggagggg agtctggtcc ggcagaccgg gcggatcctc tcgatcccgg
    29461 tgggggacgg gttcctgggt cgcgtggtgg accccctggg cgaacccctc gacggcaagg
    29521 gtcccatctc gccggacgcc acccgggccc tggaggtgca ggcgccctcg gtggtggccc
    29581 gacagccggt ggtggagccc ctgcagaccg ggctcacggc gatcgacgcc atgacgccga
    29641 tcggccgcgg tcagcgcgag ctgatcatcg gcgaccgcca gacggggaag acggccatcg
    29701 ccatcgacac gatcatcgcc cagcgggaca actggaccag cgccgacccc cacaagcagg
    29761 tccggtgcat ctacgtcgcg gtgggccaga aggcctccac ggtccgcgag gtggaggaga
    29821 ccctgcgcga gcacggcgcc atggactaca cggtcatcgt gaacgcgtcg gcatcctcac
    29881 cggcggcgtt ccagtacatc gcgccgtact cgggagccgc cctgggggcc cactggatgt
    29941 acgaggggct ccacgcactc gtgatctacg acgacctgtc gaagcaggcg gtggcatacc
    30001 gcgagatctc gctcctgctg cgccggccgc ccggccgcga ggcctatccc ggcgacgtct
    30061 tctacctgca ctcccggctg ctggaacggg cggccaagct ctccgacgag ctgggcggcg
    30121 gctccctcac cgccctcccc atcatcgaga ccaagggcaa cgacgtctcc gcgtacatcc
    30181 ccacgaacgt gatctcgatc accgacggcc agatctacct cgagcccgac ctgttcttcg
    30241 ccggcgtgcg tccggcgatc aacgtgggca tctcggtgtc ccgggtggga ggcaccgcgc
    30301 agatcaaggc catgaaggcg gtggcgggac gcctgcggct ggacctcgct cagtaccggg
    30361 ccctcgaggc cttcgcgcag ctcggatccg agctcgaccg ggcgtcgcag cagcagctgg
    30421 ctcgtggggc ccgggtggtg gagatcctga agcagcccca gtaccggccg gtgcccgtgg
    30481 agcgacaggt cctgtacatc tgggtcgcca ccggtgggca cctggacgac ctgccggtcg
    30541 aggacgcccg acggttcgtg gagggcgtgg ccgaatacgt ggaggcgcgg cacccggaga
    30601 tcctgcgggc gatccgcgaa acgggcgtgc tgtccgagga gggggaggcg atgctccgcg
    30661 acgcggtcgc cgggtacaag gagtccttcg tcccctcgac ggaaggcgag gctggtgagg
    30721 ccgaggggga ccaggggtgg cggcgggtct cctccgacca ggagctgcag cggcgtccac
    30781 ccacccagga ggagctgcgc caggactacg agcgacgggc cggtccggtc ggcgagtccg
    30841 aggcgccgat cccgtaaccc atgggcgcga agctccgcat cgttcgtcga cagatccgct
    30901 cggtccagtc gaccatgaag atcacccgcg ccatggagct
//
LOCUS       DASQQQ010000020        16839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_249310, whole genome shotgun sequence.
ACCESSION   DASQQQ010000020 DASQQQ010000000
VERSION     DASQQQ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 16839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16839
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249310"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1400
                     /locus_tag="VE646_01055"
     CDS             <1..1400
                     /locus_tag="VE646_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018848048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HZD78616.1"
                     /translation="LIGDEARRYLAGLDRAPVRHVDADRAAGSIGGDLPDEGSGAVNA
                     LREVLERSAGLLASSGPRYFHFITGGVTPAALGADWLASVVDQNSADWIGAPLTSQLE
                     AVSLRWLRDLFGLPMDWDGVLTTGATMANFVGLAAARRWWGLWHGVDVDEQGLAGLPP
                     LPVFASGYLHPSDTKALAMLGVGRERATRLVRDAAGRIDLEALERALRALDGAPAVVI
                     ASAGEVNGGGFDPVDAMADLTHEYGAWLHVDGAFGLFAAVSPRTRHLVEGIAKADSVI
                     ADGHKWLNVPYESGFAFVRDRTLLPPAFALSAAYLPSEDDPRPNFFTRGPESSRRARS
                     LAVWGTLRAYGRSGYREIVERCLDLARHLAARVDEAPDLERLAEVPLNIVCFRYRPPG
                     VPEERLDDLNRRLGEAVLEDGRVHFGTTVYEGRVAFRPAIVNWRTGEPDVELIVEVTR
                     ELGARLVAQERRTPA"
     gene            1493..2497
                     /locus_tag="VE646_01060"
     CDS             1493..2497
                     /locus_tag="VE646_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HZD78617.1"
                     /translation="MALCRTADGWSLVDGLDEALRLRHDPDTLVWIESDVTGLPEAEM
                     RKLGQEFQIDPLAIEDALSPRQRPKLEAYPGHLLLVVHQLDEDEDDQLLPRQLAAFIG
                     RGFVVLLHHGADRVVGEARRRVESAGDEVTSSDRLLHAVVDTAVDDDESIATRIAQEV
                     EVMEDEALTVARAGDRRDGRSSRRGRWPSQYRLYTLKQQITTLRRYALPIASALERRP
                     TYEGQERDDTLDRLFRDVHDHVVRLGAEVRSSDDLTDGVLELVRSFQADELNETNRKL
                     TAWAAIIAGPALIVGLYGTNYGLLPPRSVGQWGFALVLLLMATTSGLLFGFFRRRGWL
                     "
     gene            2528..3985
                     /locus_tag="VE646_01065"
     CDS             2528..3985
                     /locus_tag="VE646_01065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD78618.1"
                     /translation="MAEGTRRVGSAGSAGAEVMGETAATQAARPVPWREVFAGARGRL
                     TAGLLTLEMLVAIQILIVATIMPAVRRDLGDVALYGWTFSASGLGQFAAIPIAGHALD
                     RFGARRLLAVTLLVYSGGLLFAALAPSMLTLVVARLLQGIGGGAIYAVSLGTVAKTYP
                     DDVRARVLALLAAMWILPGLVGPGIGALFASTVGWRWAFVVPIPILVVAAALVFPALP
                     TAIDREARIPLLWPLVLATGAGAVLAGLTDLSLWSIGLIIVGSAAALPALVRIVPKGT
                     LRARPGLPAAAAAAFLLSVGFLAVDAFVPLMLTDVRGRSVAEAGIVLTLATVTWSVGS
                     WWQSSVAGRIRLGRLVQLGTLLVVGGSVATMVGMLHVPLALPYAGWAVAGLGMGIAFP
                     SIPLSVMNAAEAGAEASQLSPTLLMDTLGVAVGAGLAGACVAFAERVGASLRLGLVGG
                     FSLSLVAMLVLLPVAARIPSGRAGDRTSALAGHDA"
     gene            4057..4347
                     /locus_tag="VE646_01070"
     CDS             4057..4347
                     /locus_tag="VE646_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014011770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HZD78619.1"
                     /translation="MEISRLQEALRRTYYRRDAKRGRDATFRWLTEEVGELAKALRTG
                     DRDNLRHEFGDVLAWLTSLANLAGVDLEEAASRYAQGCPRCGRSPCSCPFVR"
     gene            4344..5534
                     /locus_tag="VE646_01075"
     CDS             4344..5534
                     /locus_tag="VE646_01075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZD78620.1"
                     /translation="MIEVRQPGEEHRDQVSEAITISLDLDRRHADGRAERLLLHHFRC
                     AFEGDRVLATAAAWPFRQWFGGRELRMTGIYAVTTLPEYRAAGLASRTTEQLLHEGRE
                     RGVPLSALYPATLRPYRRLGFELAGTFTEHTAPFDALPEASGPLAVEEYRPEDLEGVR
                     ACYRRWAAGSNGPIDAEDEAWWPTRVLGSSDPSAIHRVAVVRDDSGTVRGYVTCRKEP
                     ETGDFRASFRLACEHFVACDPEALSSLLGYVRGFRGLGVSLTIVGAPADPLGLVLAER
                     RLRPTRTMRWMLRLLDVPRALEQRGYPPVSGAATIAVEDPTFADNRGPWRIEADAGSV
                     TVTRTRSSDRRPLHIRTLSSLFSGYLSAHDAVHLGLLEADDPAVALLATLFAGPAPFM
                     LDFF"
     gene            5747..6988
                     /locus_tag="VE646_01080"
     CDS             5747..6988
                     /locus_tag="VE646_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZD78621.1"
                     /translation="MDVLIERCAGLDVHKDTVMACVRFPGEGGKQAQEIHQFGTTTAG
                     LLAPWDWLGAHGVTVVGMESTGVYWKPVSYVLEDQLECQLLNARHLRNLPGRKTDVKD
                     AEWIAQLVQHGLVRPSFVPPKPIRELRDLTRYRKAQINERIAEAQRLDKILQDAGVKL
                     YSVATEILGVSGRAMLTALVEGTRDPGVLSELARGKLRAKIPALKEALTGRFGAHHAL
                     IVGTILSKLDFLEQVISHLSVEIEQVIAPFEPQVELLDTIPGVDRRAAESIIAEIGVD
                     MGGQFGSSARLASWAGMCPGNDESAGRHRSGKTRKGSKWLRTTLTESAKAAARSKGSY
                     FSAQHARLKGRRGSRKATTTVGHSILVVAYHVLDRGVPYQDLGADYFLRRHDPKRHAA
                     KLVRQLQNIGYRVTLEEVEAA"
     gene            complement(7082..8104)
                     /locus_tag="VE646_01085"
     CDS             complement(7082..8104)
                     /locus_tag="VE646_01085"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005027838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HZD78622.1"
                     /translation="MRVAVVGATGAVGREMIRILQERAFPADELVPLASTRSEGMQVA
                     FGDASYVVHALSLDRLRDVDVALVSAGATVSRSFIPQAAGAGATCIDNSSAFRMDPDV
                     PLSIPEVNPEALEGAPRIVAVPNCTTITAMMAVAPLHRAAGLRSLVLSSYQSVSGAGH
                     KGVSELAEQVEKLHGMEDDLARMKADALPVGEVFGKTIAFNVVAKVAHFEPDGFTGEE
                     SKMMAEPRKILGLPDLPVAATAVRVPVPVGHAVSILAEFSRAVSVQEAREVLASAPGV
                     QLRDDPANGIYPSPLEAAGMDEALVGRIRRVPGRDDALLLFSCADNLRKGAALNAVQI
                     AEHLFR"
     gene            complement(8157..9395)
                     /locus_tag="VE646_01090"
     CDS             complement(8157..9395)
                     /locus_tag="VE646_01090"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017956300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HZD78623.1"
                     /translation="MSIIVQKYGGTSVAGAERIQAVADRVVAAREAGHDVVVVVSAMG
                     DTTDELAQLAHRITPIPEPRELDMLLTAGERIAMALLAIAVCARGCRAASYTGSQAGI
                     ITDTHHGKARIVDIRPKRIIESLQAGNVVILAGFQGLSTAYDITTLGRGGSDTTAVAM
                     AAALGAEACEIYTDVPGVFTADPRIVPGARKVGKITYEAMLELAASGAKVLQLRSVEY
                     ARRNDVRLHVRSSFSDGAGTWVVGEDEIGMEQALISGVALDDGEAKITLEDVPDRPGV
                     AASIFKAVAAEGVNVDMIVQNVSHAGTTDLSFTVPRDDLPRLEATVQRMIGQVGAARF
                     SADDQIAKVSLVGAGMRSDPGVAADMFDALAAEGINIEMISTSSIRVSCVIRAVDAER
                     AVRTLHMRFGLDEETSSAPT"
     gene            complement(9506..11668)
                     /locus_tag="VE646_01095"
     CDS             complement(9506..11668)
                     /locus_tag="VE646_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZD78624.1"
                     /translation="MSEPAPGSPMPEPDAFPAQAARTRGFTLGAPRDFTVSPDGSRVV
                     FLRSKAGDDPITGLWVLDTQDGTERCVFDPRENGPAEDETELSQAERARRERTRERAG
                     GVVGYATDREVRRAVFTLSGRPFLVDLMPAQDGVPGTVQALLRHRVVELPAPGVVDDP
                     RLDPEGRRIAYVVGGTLHVLDLDGPDRVLAGTDDPDRFWGLPEFIAAEEMRRLRGHWW
                     SPDGTRLAATCVDQRGVLTWHISDPTHPAARPRAVRYPQAGTEDAAVTLWVFDVDRGE
                     RVEIRWEREAFPYLARVHWSDGEPLLLSVQARDQRTVRLLEADIETGTTTLVREESDP
                     QWVDLPEEAPVRLNDGRVVWVRADRQADTYRLTVDGDPVTPAGLQIRDVISAGDGVLF
                     TASEDPLEVQLWRWTPEGLARLSAEPGVHTGREGGGTLVLVSVTMDAVLPSVSVLRNG
                     EEIARIGSLAETPVVRPRPWFLRLGERELLATLLLPEGREPDRPLPVLLDPYGGPHGQ
                     RVFRTASAHLTSQWFADRGFAVLVIDGRGMPGRGPAWDREVFGDFTVTLEDQIDGLHA
                     AAERFGFLDLSRVGIRGWSFGGELAALAVLRRPDVFHAAVAGAPVTDQTLYDTHYTER
                     YLGHPDREPEAYRRSSPIHDASKLRRPLLLIHGLADDNVVVAHTLRLSAALFEAGRFH
                     ELVLLPNATHLTRSTALTENVLRIQLDFLQRSLRHPEA"
     gene            complement(11677..12270)
                     /gene="recR"
                     /locus_tag="VE646_01100"
     CDS             complement(11677..12270)
                     /gene="recR"
                     /locus_tag="VE646_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020522904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HZD78625.1"
                     /translation="MYEGPIQDLIDELSRLPGIGPKSAQRLAFYLVKAPSEEAKRLSE
                     AILAAKERIVFCRECGNVAEAELCRICRDQSRDPTVLCVVEEPKDAATVERAGVVRGR
                     YHILGGAISPLDGIGPDDLRIQELLERVQRDGVVEVILATNPNLEGNATAMYVAGLVK
                     PLGVRVTRLASGLPVGGDLEYADEVTLSQALEGRREM"
     gene            complement(12277..14067)
                     /gene="dnaX"
                     /locus_tag="VE646_01105"
     CDS             complement(12277..14067)
                     /gene="dnaX"
                     /locus_tag="VE646_01105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HZD78626.1"
                     /translation="MAEDKPYQSLYRKYRPQGPEEVLGQEHVIRALMGAVREGRLAHA
                     FLFTGPRGTGKTSTARILAKMVNCEQGPTPEPCGTCEQCVRIREGTHLDVVEIDAASH
                     GGVDDARELREKAPTAPVQGREKVYIIDEAQRLSREAFDALLKVFEEPPAGVRFVLAT
                     TEPHKMPPTIIGRCQRFDFRRIPLEVVAGLLQRIAADEGLALAPGAAIAIARQSEGSA
                     RDALSLMDQASVLGAGGIGEDIVASLVGAGDADVQHALADAVAVGDARAVFQLIDAMV
                     QEGRDLRHVTNETLTHFRNLLLARTAPEQGELLDVTEDEAGKLRAQASKFSPAELSRV
                     LALLLAAQADMRWTTSPRLTFELALVRATIPQTDPQPQALTARIERLERLAKITPTTT
                     AQPAEGQPAPRAALEPPTAQVLREDAERPAPPQPSPEGTPQPPPAESPERPEPTATEE
                     PRAAEERAAAPGGPSQPLVGAGTAAVDVGMIRSSWGSLIAHLRSERKFVVTSFLEVAT
                     PAAYDGEVLELVFPPDRRLGATKVTEREGELRAALQALLGIAPRIHCVVRDAVAGASD
                     PEDEEPPPSEEEALARLKAELGAQATAEGE"
     gene            complement(14143..14410)
                     /gene="ffs"
                     /locus_tag="VE646_01110"
     ncRNA           complement(14143..14410)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VE646_01110"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            14489..15274
                     /locus_tag="VE646_01115"
     CDS             14489..15274
                     /locus_tag="VE646_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HZD78627.1"
                     /translation="MDPAAAGAVVAPAPENSRPLGGVALGYAQVVLPIGAILVDFDGT
                     ACSEDVSELLLERFGDPSWPLYDELVDRGEMGLREAAAHQAAMLRGSREELLAFALAN
                     APLDPTFPGFVGWASDRGVAVTLVSDGFAFYIGPILEAAGLGHLRVITNELVFDGGTP
                     RLRHPNGHPGCVGCGTCKMLPAVEARRAGAVVFVGEGQSDRYGAMYSDVVFAKDHLVQ
                     ICLDDGVPFLSWRTFDDVRSALEELDALPGAVAPATCPGWRAR"
     gene            15250..16206
                     /locus_tag="VE646_01120"
     CDS             15250..16206
                     /locus_tag="VE646_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZD78628.1"
                     /translation="MPGLAGTVSLRDVGLPDGISTRPLVGADVPTAFGLFRRAQLHDL
                     GELEIDLEDVASIFRKPSFDVGSHSVGLWHDDGLVGQCLMIRPGRAEATVDPSWRGRG
                     LGAWLVGWSEGAASRAGADRLEQAIEDGNAGARHLLVRAGYRTSHTSWLLRIELEEEP
                     GEPAPPPGIEIRDFVPRQDDRIVHRLIDEAFSSFSPSEPFEDWAADTIHRTDFTADRL
                     AVAVDGDQIVGAAVSIPYAGEGWIEQLAVRASHRHRGIGRALLRHAFRATWERGERMA
                     GLATNSKTGALGLYQDVGMRVRRSYTHWAKTLAAAPAGSEAC"
     gene            16217..>16839
                     /locus_tag="VE646_01125"
     CDS             16217..>16839
                     /locus_tag="VE646_01125"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZD78629.1"
                     /translation="MHSPHDGSTRPATARRDGDERPDPRDASSFSRRRFLKTSALAVA
                     GAAVYACTGGGRKRPVVTPTATAAVTGTDTRWPIKRVVYVMPENRSFDNIFARYPGAT
                     GTTTGVKFGEEVPLTPCPEWLPGDLPHDRAAFLNSVDGGAYDGFATGQFGDPWAYTIF
                     EEGSVPVYWQYARDYVLCDNLFASVGGPSHPNHLFFVAGQSGGVIDNP"
BASE COUNT         2283 a         5995 c         6082 g         2479 t
ORIGIN      
        1 atctgatcgg tgacgaggct cgtcggtacc tggcggggct ggaccgggcg cccgtccgac
       61 acgttgacgc cgaccgggcc gcgggatcga tcggcggcga cctgccggac gaggggtcgg
      121 gggccgtgaa cgcgctgcgg gaggtgctgg agcgctcggc aggactgctc gcctcctcgg
      181 gtccccgcta cttccacttc atcaccggcg gggtgacccc ggcggcgctg ggcgccgact
      241 ggctggcctc ggtggtcgac cagaacagcg cggactggat cggggcgccg ctcacctccc
      301 agctcgaggc ggtctctctc cgttggctca gggacctgtt cgggctcccg atggactggg
      361 acggggtcct gaccacgggc gccaccatgg ccaacttcgt ggggctggcg gcggctcggc
      421 ggtggtgggg cttgtggcac ggcgtggacg tggatgagca gggcctggcg ggcctacccc
      481 cgctgcccgt cttcgcgtcc gggtacctgc acccgagcga cacgaaggcc ctggcgatgc
      541 tgggcgtggg ccgggaacgc gccacacgcc tggtccggga cgccgccggc cgcatcgacc
      601 tggaggccct ggagcgcgcg ctgcgggcgc tggatggggc gccggccgtg gtgatcgcca
      661 gcgccggcga ggtgaacggc ggcggcttcg atccggtcga cgcgatggcc gacctgacgc
      721 atgagtacgg tgcctggctc cacgtcgacg gggcgttcgg gctcttcgcg gcggtctcac
      781 ctcggacccg tcacctggtc gagggcatcg ccaaggccga ctcggtgatc gccgacggcc
      841 acaaatggct gaacgtcccg tacgagtccg ggttcgcgtt cgtgcgggac cgcaccctgc
      901 tcccgcccgc cttcgcgctg agcgccgcgt acctgccctc ggaggacgat ccgcgtccga
      961 acttcttcac ccgaggcccg gagagctccc ggagggcccg gagcctggcc gtgtggggca
     1021 cgctgcgcgc ctacgggcgc tcggggtacc gcgagatcgt ggagcgctgc ctggacctcg
     1081 cgcggcatct cgcggcgcgg gtggatgagg caccagatct ggaacggctg gccgaggtgc
     1141 cgctcaacat cgtctgcttc cgataccggc ctcccggcgt gccggaggag cgcctcgacg
     1201 acctcaaccg gcggctgggt gaggcggtcc tggaggatgg gcgggtgcac ttcggcacca
     1261 ccgtgtacga gggccgggtg gccttccggc ccgcgatcgt gaactggcgt acgggcgagc
     1321 cagacgtcga gctgatcgtc gaggtcaccc gcgagctcgg cgcccggcta gtcgcccagg
     1381 agcgccggac gcccgcctag gctcgcggcg tgcctggtcg tcggccagcg atcgagcgga
     1441 cggctcgggt ttgccccgcc aacgtcccgg ggacatcggc gacatgcgac cgatggcgct
     1501 ctgtcgcacc gccgacggct ggtccctggt ggacggcctg gacgaggctc tccgactccg
     1561 ccacgatccc gacacgctag tctggatcga gagcgacgtt acgggcctgc ccgaggccga
     1621 gatgcggaag cttgggcagg agttccagat cgaccccctc gcgatcgaag atgccctgag
     1681 cccgaggcag cggcccaagc tcgaggccta tcccggtcat ctcctgctgg tcgtccatca
     1741 gctcgacgag gacgaggacg accagctcct gcctcgacag ctcgccgcct tcatcggtcg
     1801 cgggttcgtg gtgctcctgc atcacggggc ggaccgggtg gtgggcgagg ctcggcggcg
     1861 cgtggagtcc gctggggacg aggtgacctc gagcgaccgc ctgctccacg ccgtggtgga
     1921 cacggcggtg gacgacgacg agtcgatcgc cacgcggatc gcccaggagg tcgaggtgat
     1981 ggaggacgag gccctcaccg tggctcgcgc cggggaccga cgggacggcc ggtcctcccg
     2041 gcgggggaga tggcccagcc agtaccgcct gtacacgctg aagcagcaga tcaccacgct
     2101 tcgccgctac gcgctaccga tcgcctccgc gctggagcgc cgtccgacgt acgaggggca
     2161 ggagcgggac gacaccctgg accgcctgtt ccgcgatgtc cacgaccacg tggtccggct
     2221 gggcgcggag gttcgaagct cggacgacct gacggacggc gtgctcgagc tggtccgctc
     2281 cttccaggcc gacgagctga acgagacgaa ccgcaaactc acggcgtggg cggccatcat
     2341 cgccggcccc gcgttgatcg tcggcctcta cggcaccaac tacggcctgc tccctccgcg
     2401 gagcgttggg cagtgggggt tcgcgctggt cctgctcctg atggccacca cctcgggcct
     2461 gctgttcggg ttcttccgcc gccgcggctg gctctgaccc ggccgtcggc ttcgtaggct
     2521 aggggccgtg gccgagggaa cgaggcgggt gggatcggct gggagcgccg gcgccgaggt
     2581 gatgggggag accgcggcca cccaggcggc gcggcccgtg ccgtggcggg aggtcttcgc
     2641 cggggcgcga ggtcggctga ccgccggtct gctcaccttg gagatgctcg tcgcgatcca
     2701 gatcctgatc gtggccacga tcatgcccgc cgtccgccgg gacctcggcg acgtcgccct
     2761 gtacgggtgg acgttcagcg cctcggggct cgggcagttc gccgcgatcc ccatcgccgg
     2821 ccacgcgctg gaccggttcg gggcccgtcg gctgctcgcg gtcaccctcc tggtctactc
     2881 gggaggcctg ctgttcgcgg ccctagcgcc ctcgatgctg acgctggtgg tcgctcggct
     2941 gttgcagggc atcggcgggg gcgcgatcta cgcggtctcc ctcggcacgg tggccaagac
     3001 ctacccggac gatgtccggg cccgcgtgct ggcgctgctc gcggcgatgt ggatcctgcc
     3061 gggcctggtg gggccgggga tcggcgccct gttcgcgagc acggtgggat ggcggtgggc
     3121 cttcgtggtg ccgatcccga tcctggtcgt cgccgcggcg ctggtgttcc ccgcgctgcc
     3181 caccgcgatc gatcgggagg ccaggatccc gctgctctgg cccctggtcc tggccaccgg
     3241 cgccggagcc gtgctcgccg ggctcaccga cctgtccttg tggagcatcg gcctgatcat
     3301 cgtgggctcg gccgccgcgc tcccggccct agtgcggatc gtcccgaagg ggaccctgcg
     3361 ggcccggccc ggcctgccgg ccgcggcggc cgcggccttc ctgctctcgg tgggtttcct
     3421 ggccgtcgac gcgttcgtgc cgttgatgct gaccgacgtc cgcggccgct cggtcgccga
     3481 ggccgggatc gtgctgacgc tggccacggt cacctggtcg gtgggcagct ggtggcaatc
     3541 cagcgtggcg gggcgcatcc ggctcggtcg gctggtgcag ctcggcacgt tgctcgtggt
     3601 gggcggcagc gtcgccacga tggtggggat gctccacgta ccgctcgcgc tgccgtatgc
     3661 gggctgggcg gtggctggcc tggggatggg catcgcgttc ccctcgatcc ccctctcggt
     3721 gatgaacgcc gcggaggcgg gggctgaggc gagccagctg tcgcccaccc tgctgatgga
     3781 cacgctgggc gtggcggtcg gcgccgggct ggccggggcg tgcgtggcct tcgccgagag
     3841 ggtcggcgca tcgctccggt tggggctggt gggcggcttc tccctgagcc tggtggcgat
     3901 gctggtgctg ctgcccgtcg ccgctcggat cccgagcggc cgagccggcg accgaacctc
     3961 agcgctcgcg ggccacgacg cgtaggtacg cgttcccgtc gacgcctggc ttctcgacgg
     4021 tgtccgctca ccgagcgccg gtagactcgg cgagccatgg agatctcccg gctgcaggag
     4081 gcactgcgcc gtacctacta ccggcgcgac gcgaagcggg gacgcgacgc caccttccgc
     4141 tggctcacgg aagaggtcgg cgagctggcg aaggcgctgc ggaccggcga tcgcgacaac
     4201 ctccggcacg agttcggcga cgtcctggcc tggttgacct cgctggccaa cctggccggg
     4261 gtggacctgg aggaggccgc ctcccggtac gcgcagggct gcccccgctg cggcagatcg
     4321 ccctgctcat gccccttcgt ccgatgatcg aggtccgtca acccggcgag gagcaccgcg
     4381 atcaggtctc ggaagccatc accatctccc tggatctgga ccggcgccac gccgatggcc
     4441 gggccgagcg actgctcctg caccacttcc gctgcgcgtt cgagggcgat cgggtgctgg
     4501 ccacggcagc ggcctggccc ttccgccagt ggttcggtgg ccgggagctg cggatgaccg
     4561 gcatctacgc cgtcaccacc ttgcccgagt accgagcggc cgggctcgcg tcccggacca
     4621 ccgagcaact gctccacgag gggcgcgagc gcggcgtgcc gctgtcggcc ctgtatccgg
     4681 cgacgctgcg gccctaccgg cgcctgggct tcgagctggc cgggaccttc accgagcata
     4741 cggcgccgtt cgatgcactt ccggaggcgt cggggcccct ggcggtggag gagtaccggc
     4801 ctgaggacct ggagggcgtc cgggcctgct accggcggtg ggccgcgggg tccaacgggc
     4861 cgatcgatgc ggaggacgag gcctggtggc ccacccgcgt gctcggctcc tccgatccga
     4921 gcgcgatcca tcgggtcgcc gtggtccggg acgactcagg cacggtgcgt gggtacgtga
     4981 cctgccggaa ggagccggag accggcgact tccgcgcgtc gttccggctg gcgtgcgagc
     5041 acttcgtcgc gtgtgatccg gaggcgctgt catcgttgct cgggtacgtg cgcggcttcc
     5101 gagggctcgg cgtgtccctg accatcgtgg gagctcctgc ggacccgctc ggcctggtcc
     5161 tcgcggagcg ccggttgcgg cccacacgca cgatgcgctg gatgctccgc ctgctggacg
     5221 tcccccgagc cctcgaacag cgagggtacc caccggtctc gggtgctgcc acgatcgcgg
     5281 tcgaggatcc aaccttcgcc gacaaccgcg gtccctggcg gatcgaggcc gatgccgggt
     5341 ccgtgaccgt gaccaggacc cgatcgagcg acaggcgccc gctgcacatc cggaccctgt
     5401 cctcgctgtt cagcgggtac ctctccgccc acgatgccgt gcacctgggg ctgctcgagg
     5461 ccgacgatcc cgcggtcgcc ctcctggcca cgctcttcgc cggtcccgcc cccttcatgc
     5521 tggacttctt ctgagtgatc ctggcccttg cgggccaccc ccaacggatg aaaatgggcg
     5581 ggtcgcccgg cgacgtcggg tttgcctggt gttgcgagcc cgtttgtgag tgggacgcgg
     5641 gggcctcggg aacccacaac tgttgccgcg agcacgaacc acagactccg tgagatgaga
     5701 ctcctccggg cggcgggtcg ttgtcggtcg gaggaaggag ggaaggatgg acgtcctcat
     5761 cgagcggtgc gcaggtctgg acgtgcacaa ggacacggtc atggcctgcg ttcggttccc
     5821 gggagaaggg ggcaagcagg cccaggagat ccaccagttc ggcaccacca ccgcgggcct
     5881 gctggcccct tgggactggc tcggagccca cggggtcacc gtggtgggca tggagtccac
     5941 cggcgtgtac tggaagccgg tgtcctacgt gctcgaggac caactcgagt gccagctgct
     6001 gaacgcccgg caccttcgca acctccccgg gagaaagacc gacgtcaagg acgcggagtg
     6061 gatcgcccag ctggtccagc acgggctggt ccggccctcg ttcgtgccgc ccaagccgat
     6121 ccgggagctt cgtgacctca cccggtaccg caaggcccag atcaatgagc ggatcgcgga
     6181 ggcccagagg ttggacaaga tcctgcagga cgccggggtc aagctgtact cggtggccac
     6241 cgagatcctg ggggtctccg ggcgggccat gctcaccgca ctggtggagg ggacccgaga
     6301 tccaggcgtg ctgtccgagt tggcccgggg caagctccgg gcgaagatcc cagccctgaa
     6361 agaggccctg accggacggt tcggagcgca tcacgccctg atcgtgggga ccatcctgtc
     6421 caagctggac ttcctggagc aggtcatctc ccacctgtcg gtcgagatcg agcaggtgat
     6481 cgcccctttc gagccccagg tggagctgct tgacaccatc ccgggggtgg accggcgcgc
     6541 ggcggagtcg atcatcgccg agatcggggt ggacatgggc ggccagttcg ggtcctccgc
     6601 acgcctggcc tcctgggccg ggatgtgccc cggcaacgac gagtcggcgg gcaggcatcg
     6661 ctcgggcaag acccgcaagg gctccaagtg gctccgcacg accctcaccg agtccgccaa
     6721 ggcggccgct cggtccaagg gctcgtactt ctccgcccag cacgccaggc tcaagggccg
     6781 acgaggatca cgcaaggcga ccaccaccgt tggccactcc atcctggtcg ttgcctacca
     6841 cgtgttggat cgcggtgttc cctaccagga tctgggggcc gactacttcc tccgccgaca
     6901 cgaccccaag cggcacgccg ccaaactcgt tcgtcagctg cagaacatcg gctatcgcgt
     6961 cacccttgag gaggtggagg ccgcgtagcg atcgggggca ttttcaccac aggccgaaac
     7021 gggtctgggg caacgacggg tcaacgcctg ccggatcgat atcgtgtccg ccccccgggg
     7081 atcagcggaa caggtgctcg gcgatctgga cggcgttcag cgcggcaccc ttgcgcaggt
     7141 tgtcggcgca gctgaacagg aggagcgcgt catcgcggcc cggaacccgg cggatccggc
     7201 ccaccagggc ctcgtccatg ccggcggcct ccagggggct ggggtagatc ccgttggccg
     7261 ggtcgtcccg gagctggacg ccgggcgccg aggccagcac ctcgcgggcc tcctgcaccg
     7321 agaccgcccg ggagaactcc gccaggatcg acacggcgtg gccaacaggc accggcaccc
     7381 gcaccgcggt ggcggccacc ggaaggtcgg gcaggccgag gatcttccgt ggctcggcca
     7441 tcatcttcga ctcctcgccg gtgaacccgt ccggctcgaa gtgggcgacc ttcgccacca
     7501 cgttgaaggc gatcgtcttg ccgaagacct cacccaccgg cagggcgtcg gccttcatcc
     7561 gggccaggtc gtcctccatc ccgtgcagct tctcgacctg ctccgcgagc tcggatacgc
     7621 ccttgtgtcc ggcgcccgag accgactggt aggacgaaag caccagcgag cggagccctg
     7681 ccgcccggtg cagcggggcc accgccatca tcgccgtgat cgtggtgcag ttcggcacgg
     7741 ccacgatcct gggagccccc tccagcgcct ccgggttgac ctcggggatc gacagcggca
     7801 cgtcggggtc catcctgaac gcgctcgagt tgtcgatgca ggtggccccg gccccggccg
     7861 cctgcgggat gaacgaacgg gacaccgtgg cgcccgccga gaccagcgcc acgtccacgt
     7921 ctcggagccg gtccagggac agagcgtgga ccacgtagga ggcatcgccg aacgccacct
     7981 gcatgccctc ggagcgcgtg gaggcgagcg gcacgagctc gtccgccggg aaggcccgct
     8041 cctgcaggat gcggatcatc tcccgtccca ccgcaccggt cgctccgacc acggctacgc
     8101 gcatggtcca tccatcgtac cggcgccgcc ctcccggtcc tcggcaggtc ggccgatcag
     8161 gtgggcgccg agctggtctc ctcgtccagc ccgaagcgca tgtgcagggt gcggacggcg
     8221 cgctcggcgt ccacggcccg gatcacgcag gagacacgga tcgaggaagt agagatcatc
     8281 tcgatgttga tcccctcggc ggccagggcg tcgaacatgt ccgcggccac gccgggatcg
     8341 ctgcgcatcc cggcccccac cagcgagacc ttcgcgatct ggtcgtcggc cgagaagcgg
     8401 gccgccccca cctgcccgat catcctctgg accgtcgcct ccagcctggg caggtcgtcc
     8461 cgggggaccg tgaaggaaag gtcggtggtg ccagcgtggg agacgttctg gacgatcatg
     8521 tccacgttca cgccctcggc cgccacggcc ttgaagatcg aggccgccac gcccggacgg
     8581 tcggggacgt cctcgagggt gatcttcgcc tcgccgtcgt ccagcgccac gccggagatg
     8641 agcgcctgct ccatgccgat ctcgtcctcc cccaccaccc acgtgccggc cccgtccgag
     8701 aacgaggagc ggacgtggag gcgcacgtcg ttgcgccgag cgtactcgac ggaccgaagc
     8761 tgcaggacct tcgcgcccga ggccgccagc tcgagcatgg cctcgtaggt gatcttcccc
     8821 accttccgag cgccggggac gatcctcgga tcggccgtga acaccccggg cacatccgtg
     8881 tagatctcgc acgcctcggc cccgagcgcc gccgccatgg ccaccgccgt cgtgtcggac
     8941 ccgcctcgac ccagcgtggt gatgtcgtag gccgtggaca ggccctggaa cccggccagg
     9001 atcaccacgt tgccggcctg cagggactcg atgatccgct tggggcggat gtccacgatc
     9061 ctggccttgc cgtggtgggt gtccgtgatg atcccggcct gggagccggt gtaggaggcg
     9121 gccctacagc cacgggcgca caccgcgatg gccagcagcg ccatcgcgat ccgctccccg
     9181 gcggtgagca gcatgtcgag ttctcggggc tcggggatcg gcgtgatccg gtgggccagc
     9241 tgcgcgagct cgtccgtggt gtcgcccatg gccgagacca cgacgaccac gtcgtgcccg
     9301 gcctccctcg ccgccaccac tcggtcggcc accgcctgga tccgctcggc accggccacc
     9361 gaggtgccgc cgtacttctg cacgatgatc gacacgcagc atctcctcgc ccgctgggac
     9421 gagatgaggc taacaggcag gtccgcgagg ggtgggagct tcgacccggg cgtccgggcg
     9481 cgtgccggcg ggcggcgcgg cgggctcagg cctccgggtg gcggagcgat cgctgcagga
     9541 agtccagctg gatccggagc acgttctcgg tgagggcggt ggatcgcgtg agatgggtgg
     9601 cgttcggcag cagcacgagc tcgtggaacc tgccggcctc gaacagcgcg gccgagagac
     9661 ggagcgtgtg ggcaaccacc acgttgtcgt cggccaggcc gtggatcagc aacagggggc
     9721 gccgtagctt cgaggcgtcg tggatcggcg agctgcggcg gtacgcctcg ggctcccggt
     9781 ccgggtggcc caggtaacgc tcggtgtagt gcgtgtcgta gagggtctgg tcggtgaccg
     9841 gcgcgccggc caccgccgcg tggaagacgt cggggcgccg caggacggcg agcgcggcca
     9901 gctcgccgcc gaacgaccac ccccggatcc ccacgcgaga caggtccagg aacccgaacc
     9961 gctcggccgc cgcatggagg ccatcgatct gatcctccag cgtgaccgtg aagtcgccga
    10021 agacctcgcg gtcccaggct gggccacggc cgggcatccc tcgaccgtcg atcaccagca
    10081 ccgcgaaccc ccgatccgcg aaccactgcg aggtgaggtg cgccgaggcg gtccggaaga
    10141 cgcgttggcc gtggggcccc ccgtacgggt ccaggagcac ggggagcggg cgatcgggct
    10201 cgcgaccctc gggcaggagc agggtggcca ggagctcccg ctcccccagg cggaggaacc
    10261 agggccgcgg ccgcaccacg ggcgtctcgg ccaggctccc gatccgcgcg atctcctccc
    10321 cgttccgcag cacggagacg ctcggcagta ccgcatccat cgtcacggag accaggacca
    10381 acgtgccgcc gccctcccgt ccggtgtgga cgcccggttc cgcgctcagg cgggccaggc
    10441 cctcgggcgt ccaccgccac agttgaacct cgagcgggtc ctcggaggcc gtgaacagca
    10501 cgccgtcgcc ggcagagatc acatcgcgga tctgcaggcc ggcgggcgta acgggatcgc
    10561 cgtccacggt gaggcggtaa gtatcggcct ggcgatcggc ccgaacccag accacgcggc
    10621 cgtcgttcag gcgtaccggt gcctcctcag gcaggtccac ccactgaggg tcggactcct
    10681 cacggaccag cgtggtggta cccgtctcga tgtcggcctc gagcagccgc acggtccgct
    10741 ggtcccgggc ctggaccgag agcagcagcg gttccccatc gctccagtgc acccgtgcca
    10801 ggtacgggaa cgcctcgcgc tcccagcgga tctccacgcg ctcgccgcga tccacgtcga
    10861 agacccagag ggtcacagcg gcatcctcgg tcccggcctg cgggtagcga acggcgcgag
    10921 ggcgagccgc cggatgggtg ggatcggaga tgtgccaggt gagcaccccc cgctgatcca
    10981 cgcaggtggc ggccagccga gtgccatcgg gtgaccacca atggccccgg aggcggcgca
    11041 tctcctcggc ggcgatgaac tcgggcaggc cccagaagcg gtcggggtca tcggtcccgg
    11101 cgagcacgcg gtcgggacca tccagatcca gcacgtggag ggtcccgccg accacgtagg
    11161 cgatgcggcg gccctccgga tccagccgcg gatcgtccac cacgccgggc gcgggcagtt
    11221 cgacgacgcg gtggcgaagg agcgcctgga ccgtccccgg gaccccgtcc tgagccggca
    11281 tgaggtccac caggaacggt cggcccgaca gcgtgaacac ggcccggcgc acctctcggt
    11341 cggtggcgta gcccaccacc ccaccggcgc gctcgcgggt tcgctcccgg cgggcccgct
    11401 cggcctggga cagctccgtc tcgtcctccg ccgggccgtt ctcgcgcggg tcgaacacgc
    11461 agcgctcggt cccatcctgc gtatcgagca cccacaggcc cgtgatcggg tcgtcgccgg
    11521 ccttcgagcg gaggaagacc acccgggatc cgtccggtga gaccgtgaag tcccgaggcg
    11581 caccgagcgt gaacccacgg gtccgagcgg cttgggccgg gaacgcgtcg ggttcgggca
    11641 tcggcgaccc gggagcgggc tcgctcatcg ggggcctcac atctcccggc ggccctcgag
    11701 ggcctgggag agcgtgacct cgtcggcgta ctcgaggtca ccgcccacgg gcagaccgct
    11761 ggccagacgg gtgacccgca cgcccagggg cttcaccagc cccgccacgt acatcgcggt
    11821 ggcgttgccc tccaggttgg ggttggtagc aaggatgacc tcgaccacac cgtcgcgctg
    11881 cacccgctcc agcagctcct ggatccgcag gtcgtcgggc ccgatcccat ccagtggcga
    11941 gatggccccg cccaggatgt ggtagcggcc gcggaccacc ccggcccgct cgacggtggc
    12001 ggcgtccttc ggctcctcca ccacgcagag caccgtggga tcgcggctct gatcgcggca
    12061 gatccggcag agctcggcct cggccacgtt gccgcattcc cggcagaaca cgatgcgctc
    12121 cttggccgcc aggatggcct cggagaggcg cttggcctcc tccgagggcg ccttcaccag
    12181 gtagaaggcc agccgctggg cggacttcgg accgatcccg ggcagtctgg acagctcgtc
    12241 gatcaggtcc tggatcggtc cctcgtacat ggtggcctac tcgccctcgg ccgtcgcctg
    12301 ggcaccgagc tcggccttca ggcgggccag cgcctcctcc tccgagggtg gcggctcctc
    12361 atcctccggg tccgaggcgc cggccacggc gtcccggacc acgcagtgga tccggggggc
    12421 gatgcccagc agcgcctgca gcgcggcccg cagctcgccc tcgcgctcgg tgaccttggt
    12481 ggctccgagc cgccggtccg gcgggaagac cagctcgagg acctcgccgt cgtaggcggc
    12541 cggcgtggcc acctccagga aggacgtgac cacgaacttg cgctccgacc gcaggtgggc
    12601 gatcaaggac ccccaggagg agcggatcat ccccacgtcc accgcggcgg tcccagcccc
    12661 cacgaggggc tgggaaggcc ccccgggtgc cgccgcgcgc tcctcggctg cccgtggctc
    12721 ttcggtcgct gtcggctcgg ggcgctcggg cgactcggcc ggtggaggct ggggcgttcc
    12781 ctccggtgac ggctggggcg gtgctgggcg ctctgcgtcc tcacggagga cctgtgccgt
    12841 aggcggctcc agagccgctc tcggggccgg ctgcccctcg gcgggctggg cggtggtggt
    12901 cggcgtgatc ttggccagcc gctccagccg ctcgatgcgg gcggtgaggg cctgaggctg
    12961 gggatcggtc tgggggatcg tggcccggac caacgcgagc tcgaacgtga gcctgggcga
    13021 ggtggtccag cgcatgtccg cctgcgcggc cagcagcagc gccagcacgc gggagagctc
    13081 ggccggggag aacttgctgg cctgagcccg gagcttgccg gcctcgtcct ccgtcacgtc
    13141 cagcaactcc ccctgctcgg gcgccgtccg cgccaggagc aggttgcgga agtgggtgag
    13201 cgtctcgttg gtgacgtgac gcaggtcgcg accctcctgc accatcgcgt cgatcagctg
    13261 gaacaccgcc cgcgcatccc ccacggccac ggcgtcggcc agcgcgtgtt gcacatccgc
    13321 gtcccccgcc cctaccagtg aggccacgat gtcctcgccg atcccgccgg cccccaggac
    13381 ggatgcctgg tccatgagcg agagcgcgtc ccgggccgag ccctcggact gccgggcgat
    13441 cgcgatggcc gccccgggcg ccagcgcgag cccttcgtcc gccgcgatcc gctgcagcag
    13501 ccccgccacc acctccagcg ggatccggcg gaagtcgaac cgctggcagc gcccgatgat
    13561 ggtggggggc atcttgtgtg gctcggtggt ggccaggacg aagcgcaccc cggccggcgg
    13621 ctcctcgaag accttcagta gggcgtcgaa cgcctcccgc gagagccgct gagcctcgtc
    13681 gatgatgtag accttctccc gaccctggac cggcgccgtg ggcgctttct cccggagctc
    13741 tcgcgcgtcg tccacgccac cgtgggaggc ggcgtcgatc tcgaccacgt ccaggtgcgt
    13801 gccctcccgg atccgcacgc actgctcgca ggtcccgcac ggctcgggcg tgggcccctg
    13861 ctcgcagttg accatcttgg ccaggatccg tgcggtggag gtcttgccgg tcccccgcgg
    13921 gcccgtgaac aggaaggcgt gcgccagccg gccctcgcgc acggccccca tcagggcgcg
    13981 gatcacatgc tcctggccca ggacctcctc agggccctgc ggccggtact tgcggtagag
    14041 cgactggtac ggcttgtcct cggccacgtg cctccccagg catcggtcct ccccgcacgg
    14101 gggaagacga gcgggcgcgt gagcacccaa gtctagtggt ggccgtgcac ccgccgttga
    14161 ccggggatgg acccgaccgc atcaccggcc ggtggctccg gtcaggcgac cccacggcac
    14221 ccgccagcgc ccgcttacgg ctgcttcctt ccggatctga ccgggttcgc aggttggcgt
    14281 cgcgtgggac ccgaccttca tcgctccgtg ccgggagcgg gctcggaccc atcgggccgc
    14341 cggcggggat tcagccccgc tgaagcggat cgcgggttac agggcaccgc tggctcccca
    14401 cctagcacgg ccaccagggg gaagtatacg tgggggggtg accgacctca ggggcctccg
    14461 aggtgcggcg tagcctgccc acgatccggt ggatcccgct gccgccggag cggtggtcgc
    14521 tcccgccccc gagaactccc ggccgctcgg cggggtcgcg ctcgggtacg ctcaggtcgt
    14581 gctcccgatc ggcgcgatcc tggtggactt cgacggcacg gcctgcagcg aggacgtctc
    14641 cgagctgctg ctggagcggt tcggggaccc gtcctggccc ctctacgacg agctggtgga
    14701 ccggggggag atgggccttc gggaggcggc ggctcaccag gcggcgatgc tccgaggctc
    14761 ccgggaggag ctgctggcgt tcgccctggc caacgctccg ctcgacccca cgttccccgg
    14821 attcgtgggg tgggcatcgg atcggggggt ggccgtgacg ctggtctcgg acgggttcgc
    14881 cttctacatc ggaccgatcc tggaggcggc ggggctgggc cacctgcgcg tgatcacgaa
    14941 cgagctggtc ttcgatggag gaacgccccg actgcggcat cccaacggtc acccgggatg
    15001 cgtggggtgc gggacctgca agatgctccc cgccgtggag gcgcgccggg ccggggcggt
    15061 ggtcttcgtg ggcgagggcc agtccgaccg ctacggcgcg atgtactcgg acgtcgtgtt
    15121 cgccaaggac cacctggtcc agatctgcct ggacgacggc gtgccgttcc tgtcctggcg
    15181 gacgttcgac gacgtccgat cggctctcga ggagctggac gcgctgcccg gagcggtggc
    15241 accggcaaca tgcccgggct ggcgggcacg gtgagcctgc gggacgtggg gttgccggac
    15301 gggatctcca cccggccgct cgtcggcgcg gacgtgccga ccgccttcgg gctgttccgc
    15361 cgggcccagc tccacgacct gggcgaactg gagatcgacc tggaggacgt ggcctcgatc
    15421 ttccgcaagc cctcgttcga tgtcggctcg cacagcgtgg gtctctggca cgatgacggg
    15481 ctggtcgggc agtgcctgat gatccgtccc ggcagggccg aggcgacggt cgacccctcg
    15541 tggcggggcc ggggcctcgg cgcgtggctg gtcgggtgga gcgagggcgc ggccagtcgg
    15601 gccggagccg accggctgga gcaggcgatc gaggacggca acgcgggggc gcggcacctg
    15661 ctcgtccggg ccgggtaccg gacctcgcac acctcctggc tgctccggat cgagctcgag
    15721 gaggaaccgg gggagccggc gccaccgccg ggcatcgaga tccgcgactt cgtcccgagg
    15781 caggacgatc gcatcgtcca ccggctgatc gacgaggcgt tctcctcctt cagcccgtcg
    15841 gagccgttcg aggactgggc cgccgacacg atccaccgga ccgacttcac cgcggaccgg
    15901 ctcgccgtgg cggtcgacgg cgaccagatc gtgggagcgg ccgtgtcgat cccgtacgcg
    15961 ggcgagggat ggatcgagca gctcgcggtg agggcctcgc accgtcaccg agggatcggc
    16021 cgcgccctcc tgcggcacgc gttccgagcg acgtgggagc ggggcgagcg gatggccggg
    16081 ctggcgacga actccaagac cggcgccctg gggctgtacc aggacgtggg catgcgcgtt
    16141 cgccgctcgt acacccattg ggccaagacc ctcgctgccg ctccggccgg gtccgaggct
    16201 tgctagcatc tcgcccgtgc acagcccgca cgacggcagc acccgtccgg cgaccgcacg
    16261 ccgggatggc gacgagcgcc cggatccccg cgatgcttcc tccttctccc gccggcgctt
    16321 cctgaagacc tcggcgctgg ccgtcgccgg ggccgccgtc tacgcctgca ccggcggagg
    16381 caggaaacgc cccgtggtca cccccaccgc gacggcagcg gtcaccggca ccgacacgcg
    16441 atggccgatc aagcgcgtcg tctacgtgat gcccgagaac cgcagcttcg acaacatctt
    16501 cgcccggtac ccgggcgcga cggggaccac cacgggggtg aagttcggtg aggaggtgcc
    16561 gctcactccg tgccccgagt ggctccccgg ggacctgccg cacgaccggg ccgcgttcct
    16621 gaacagcgtc gacggcggag cgtacgacgg cttcgccacg gggcagttcg gcgacccgtg
    16681 ggcctacacg atcttcgaag agggctccgt gccggtctac tggcagtacg cgcgggacta
    16741 cgtgctctgc gacaacctct tcgcgtcggt gggcgggccc tcgcacccca accacctgtt
    16801 cttcgtcgcg ggccagtccg gaggcgtgat cgacaaccc
//
LOCUS       DASQQQ010000021        21139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_29518, whole genome shotgun sequence.
ACCESSION   DASQQQ010000021 DASQQQ010000000
VERSION     DASQQQ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 21139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21139
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29518"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VE646_01130"
     CDS             complement(<1..313)
                     /locus_tag="VE646_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78630.1"
                     /translation="MTRATGNGRRIVGATLASLLVLSTSLALSGTSFASPSKQDVEAA
                     KAKLDALNQHLSQLDEQINQERIRLASIQSKLADVRRQKATAQATAQRAIADLNARAS
                     KA"
     gene            complement(447..523)
                     /locus_tag="VE646_01135"
     tRNA            complement(447..523)
                     /locus_tag="VE646_01135"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(487..489),aa:Pro,seq:cgg)
     gene            630..2027
                     /gene="fumC"
                     /locus_tag="VE646_01140"
     CDS             630..2027
                     /gene="fumC"
                     /locus_tag="VE646_01140"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HZD78631.1"
                     /translation="MNGSAVRVESDSMGTIEVPADRYWGAQTQRSLQHFSIGEDRMPL
                     EVVRAFGILKKACALVNRDLGKLAADKADLIVRAADEVIDGTLNEHFPLFVWQTGSGT
                     QSNMNANEVISNRAIELAGGELGSKTPVHPNDDVNMSQSSNDTFPTAMHVAAATAVVQ
                     RLVPAVARLRDALQRKSEEFADIVKIGRTHLQDAVPLTLGQEFSGYVAQLDADIARLE
                     QALPAVEELAIGGTAVGTGLNAPAGFGERVAAKISEMTGLPFTGAPNTFAALAAHDAM
                     VTIHGAIRTLAVSLMKIANDIRWLGSGPRSGLGELELPENEPGSSIMPGKVNPTQSEA
                     MTMVCCQVFGNDVAVTVAGSQGNFELNVFKPVMIRNVLHSVAIVSDVCHTFREFCVEG
                     IRPNRPRIARLVNDSLMLVTALSPKIGYDKAAEIAKKAHQGGTSLKEAALALGYLTER
                     EYDETVRPERMVGPD"
     gene            complement(2030..2917)
                     /locus_tag="VE646_01145"
     CDS             complement(2030..2917)
                     /locus_tag="VE646_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZD78632.1"
                     /translation="MLIRVLLAIVFAGILCVLYGIFIERRWFRLRRYRLDILPRGGSA
                     GLTVLHLSDLHLTRRAAAMRRFLSRLPGADVTAITGDLVGEPEAVEAVVEALRPVRGR
                     IASYVVLGSNDYYAPRPLNYLRYFLKHRPRRRARRGRADDLVRLLEADGWIHLRNRRI
                     EATLDGLEVEVLGLDDPHIGRQDLRAAPRACPDRFGLAVVHSPDPLPELSALGWDLAV
                     FGHTHGGQVRLPLVGALVTNSHMPRRIVSGLIRAGNTWVHISPGLGTSKYAPFRFFCR
                     PEAALLELRPAPSGSGGQA"
     gene            2933..3388
                     /locus_tag="VE646_01150"
     CDS             2933..3388
                     /locus_tag="VE646_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HZD78633.1"
                     /translation="MSDPSLKDRIRQDMTAAMKAGDRLRLGALRMLSTAITNREKEVL
                     HELSDDEVRQVAAREVKRRNESIEAFQKGGRADLVEKESAEREMLAPYAPEQLSEQEL
                     DALIDEVVEATGASGPRDLGKVMGQVMGRAKGRVDGSRVQAKVRGRLGA"
     gene            complement(3424..5862)
                     /locus_tag="VE646_01155"
     CDS             complement(3424..5862)
                     /locus_tag="VE646_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HZD78634.1"
                     /translation="MQASSRARSRRVEAPARRTVRLPKDPLYPILLFLVIGLASALVA
                     VTLSPIAGVVSMGADALNARLDQAGASFTRVPHFPERSTIYAADGSVLATLYLDENRE
                     IVRLKQVAPIARKAVLAIEDDGFYQHGALNVPSVFRALLANLIAGRITQGGSTITQQL
                     VKNAVIQDTEQTFARKFQEAALAIRLEQKYSKDQILALYLNEVYFGNGAWGIGTAAET
                     YFGKPASALNLPQAALLAGLIQAPGDYDPVKHPQAALRRRNEVLTRLGALGWVPQARV
                     DAAIASTIRLAKTAGTTGQKVQPFFVHYIVQSILEDADGRFDAFGKTYEQRRHTLFQG
                     GLRIYTTLDPDWQRYAQEAVDRSPNIDMDRGPDTSLVSVDARTGAIRAMLSGKDYRRD
                     QLDLAWHGMRQVGSAFKPFTLVAAFEHGFPAGKVYPSKSPYCSPSWRSADHCVSNAEG
                     PGNEGYIDLWRATEDSINVVFAQLALDVGPENIVSAAHQMGITTPLQAVPSITLGTEE
                     VSTLDMASAYSTLANGGVHCQPYAISKVLLPEGKKLYQHHRQCKQAIDPDIAHLVTAM
                     MQRVVCCGTGTAAQIGRPVAGKTGTGQDYTNVYFAGFTPQVATAVWVGFPQGNVPMSG
                     YYGHSVFGGTLAAPIWHDFMVKALAGMPVASFPGPPPPQYGKVPRVVGLRSLEAQKKL
                     ADANFTPIIEKIDSSLPLNTVIAQTPRAGASLALGGGVKLQVSNGKGDLIEVPRVVGM
                     DRDQAVKAVEETGLVVQIRFVDVEDPKQDGLVLAQTPVGGKQAATGSTVVLEVGRSKA
                     SPGPTPPPTPAPSG"
     gene            5917..7269
                     /locus_tag="VE646_01160"
     CDS             5917..7269
                     /locus_tag="VE646_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HZD78635.1"
                     /translation="MRRLTALFPLLLLALALAIPAAQAQSEERAPVIVAKIDGSIDRT
                     LAGFLTDTLDQAQRTGATVVLELDSAGTLDRDGIDLARRIFRSRVPVIVWVGPTPAKA
                     QGAALLFLYAAALPAVAPGAGIGPLEPLDLVDARQATMPDPAEVRRRAAAWAAARGRG
                     NVSFPATPIPAQRAIDERVAAVAAPSVTDLLDRIDGTTVTTEAGEVTLHTKIATEAGQ
                     ATVDVRFTDPGPVDRVLHAMASPAAIYVLLVFGFAALAFELTQPGFGFAGFSGLAMMA
                     LAAYGLTAVPVSWLGLGLIVAGVVLLEADVLLRKLGPLSGLGVTAFAGGSLLAFRAVA
                     PAIRLSPWLIGSLSAASLLYYGFALTVALQSRDRITSSQKGLVGLVGEARGDLEPEGP
                     VFVKGTLWRGRSADGPIPSGARVRVRGVDGLILRVEADPDPAGSPPEGWPEDPGSALP
                     "
     gene            7400..7669
                     /locus_tag="VE646_01165"
     CDS             7400..7669
                     /locus_tag="VE646_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HZD78636.1"
                     /translation="MSVRWTWAWQLEAACRGEDASLFFAPNYFEKREDKLAREAKAKR
                     SCARCRVRDECLEYALRTREPHGIWGGLNEFERRQVLRERALRAG"
     gene            7768..8238
                     /locus_tag="VE646_01170"
     CDS             7768..8238
                     /locus_tag="VE646_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZD78637.1"
                     /translation="MVENIEDRLASLKIELPPAPPPVASYVPVRIVGDLAHLSGQIAM
                     VDGEPIHRGRVGGDVSLEQGQEAARRCVLQALAALRDVAGSLDRVEAIVQLSVFVASE
                     DGFNDQPKVANGASDLLVEIFGDAGRHARVAVGVGELPLGAPVEVGMVVHLMPR"
     gene            complement(8260..10194)
                     /gene="selB"
                     /locus_tag="VE646_01175"
     CDS             complement(8260..10194)
                     /gene="selB"
                     /locus_tag="VE646_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HZD78638.1"
                     /translation="MTRDGEPAAHPERGAGLDQGGASPGSPLHVVATAGHVDHGKSSL
                     IVRLTGIDPDRWEEEKRRGLTIDLGFAWFTLPSGREIGFVDVPGHERFVRNMLAGVGS
                     VRLVLFVVAADEGWKPQSEEHLAIVDVLGAQGGVVALTKRDLVDDDELAVREREIRER
                     LEGTVLEGAPVVPCSSVTGDGVDDLISALDAMVGAAPPAEDRGRAREFVDRVFTIKGA
                     GTVVTGTLTGGALEVGQEVELYPVGIRARIRSLQTHKRSIDRARPISRVAANLAGAER
                     DRVERGDVLGLPGQWRSTTSIEARLRPVRGLVHPITNRGAYKIYAGSAERDARIRLYG
                     SGELDAGSPGAFARIRLSAPLVLDVHDRFVLRESGRRETVAGGVVLDVDPPGRPGPSP
                     QDRLSAREGASRGELPGLVVQERGAVPVADLAALAGPVPEAMPDARLIGGWWVAGSLA
                     SAVERAVHEGLRAFHDANPLQQGQDLVGVRSSVAAALRGAGRAPASALVEALLDDLDV
                     RGVVVREGRMVRLAAHRVSLSGHEDEVRRLVDAVAAGEPTPPTISELTAVGFSREVIE
                     AAARAHALVRIGAQLIMTPAFIERAEEVVRQATDGLTVSTFRARLGTSRKFAVPLLER
                     FDQRGVTIRRGDLRFPRPEA"
     gene            complement(10184..11572)
                     /gene="selA"
                     /locus_tag="VE646_01180"
     CDS             complement(10184..11572)
                     /gene="selA"
                     /locus_tag="VE646_01180"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HZD78639.1"
                     /translation="MGATPGLRRSIPSVDALLRSEPGNRASATFGRALVKRAVAQTID
                     EVRERAEGGSPPPDTDVILARALERVSYTAAGLTEVINATGVVLHTGLGRAPLPEQAV
                     EAVRRTAGGYSDLEVDRASGKRGTRSARAETLLTALTGAEAALVVNNGAAALLLALSA
                     LARRKEVLVSRGELIEIGGEFRIPDIMAASGAKLVEVGTTNRTRLSDYRAALGDRTAA
                     VLKVHPSNYRVVGFTAAPATASLASLAHQRALPFLFDLGSGLLHRYPGVPDDEPSAHE
                     ALRDGADLVIFSGDKLMGGPQAGIVLGTEELVTRMRRHPIARPLRVDKMQTAALEAVL
                     AAYARGERDHLPIWRMLQEAASTVKARAERLSLALDGDLEGAHVVECGSAVGGGSMPG
                     FTVRSWGVHLKVPHPPAFAARLRTGRPPVFCRTAEDGVLFDLRTVAEERTADLARAVG
                     YALEGDELPDDA"
     gene            11650..12852
                     /locus_tag="VE646_01185"
     CDS             11650..12852
                     /locus_tag="VE646_01185"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HZD78640.1"
                     /translation="MDTRERAEERVAFSTAAEHWMVRGGGIDVEVVSPNGLRTEEMIL
                     NIGPQHPSTHGVLRVVLELDGEVITRAEPVIGYMHRAAEKLSEHRDARQVLVLMNRHD
                     WLSAFNNELGWVLAVERLAGIDVPERARWIRTMMAEWNRILNHLMFTGSFPLELGAMT
                     PMFYAFREREMIQHLMESATGARMHHSYARVGGLKDDLPRGFLKQSAEVLKWVRKKLR
                     DFDQLIMGNEIIFARTKGVGVIPPEMALAYGCSGPVLQGSGIGMDPRKDEPYEKYGEV
                     EFDVPVGEHGDAYDRLWVLVQRMWESCRIIEQCMDRLPPGPYIAPKLPKKLNLDGEIY
                     VRTENPLGLMGYYLVGDGKKYPYRLKMRTASFSNVSVLAAMLPGTLVPDLIAVLGSIF
                     FVVGDVDR"
     gene            complement(13099..14199)
                     /locus_tag="VE646_01190"
     CDS             complement(13099..14199)
                     /locus_tag="VE646_01190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZD78641.1"
                     /translation="MSEEVASRVREAIAAHGPITFAEYMDIALYGRGGFYERPPVGAE
                     GHFVTAPHVHPVFAELLLRGLRELWEGLGRPDPLRVVEVGAGDGTLCRDLLRLSTDVG
                     PAIELWAVERSSGARRALGALGIHVAERLDELPPLDPGLVFANELLDNLPFRRVRGTE
                     RGPVEVLVDATDERFLEVTGPGDDELAPHLPALAPGEEAAVPQDALMFMDELADALVT
                     GYALLIDYGASSHPIGEVHGYRAHAVVEDVLADPGSADITAGVDFGALTERAADRGLI
                     LLGEVSQTDALMALGLGAWNEGQLQRQVASIREGSGGRSVAIWQGRSRASLLVDPSGL
                     GRLRWLLLSNPGLPAPSWLGAGSERRSRPSAD"
     gene            14310..14957
                     /locus_tag="VE646_01195"
     CDS             14310..14957
                     /locus_tag="VE646_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZD78642.1"
                     /translation="MEGAIRKAASLIAVRRAGEGPEVLVVERGAQSRFLPGYVAFPGG
                     ATDVGDADLATRWFGSSVEAPRACAVRELAEEVGLVLTAQGLRAGTRGTLDDPAASPP
                     TVDQLPRIAHWVAPEDVPVRFDADYFAVAAEAGIEPEPDGGEVARAWWASPGRLLAEW
                     AEGTRRLYWPTYFTLQHLAACARMEDLLALRIPTREADDHEVARLPRSIFWQDEG"
     gene            14961..15821
                     /locus_tag="VE646_01200"
     CDS             14961..15821
                     /locus_tag="VE646_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZD78643.1"
                     /translation="MRVVRVLAPNPGLRELDGTNTWVVGRWPAAVIDPGPDDAGHLTE
                     VVRAAGRVSLVLLTHDHPDHAPGAAPLAAITGAPVLSARGEEGGHLRDGERLTMPGVR
                     LQVVAAPGHSPDHVVFFGPEDGALFTGDAVLGRGTSLIDPPEGDLVAYLRSLHRMRDL
                     HPRTIHPGHGPVVLDAVGKLDQYLHHRAEREEQVVAALRAGMRTPEEIVPTIYGDHPQ
                     ELHELAARSVLAHLLKLETEGRAAKRLRDGIPRWRLQEPRTCERCGRPVQGRGRLCPS
                     CSMAVLQEEA"
     gene            complement(15818..16228)
                     /locus_tag="VE646_01205"
     CDS             complement(15818..16228)
                     /locus_tag="VE646_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78644.1"
                     /translation="MDGYEASTYGDRIADFYDPRYGAVPSSGPVEDTVTFLEGLAASG
                     PALELGIGTGRVAPPAAAVVAVQRIDASEQMVERMRGKPGGAGIPVAGTPGLPSSTAR
                     LAGLRLRERWGGWKREPYTGEGKHVSGWERESAP"
     gene            complement(16272..16508)
                     /locus_tag="VE646_01210"
     CDS             complement(16272..16508)
                     /locus_tag="VE646_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZD78645.1"
                     /translation="MPQPVRTGASARHAVRPGVQLTVPLTQEHLGRMVGASREAVNRS
                     LVTLSRQGLVRTQDRRFVIADLPALTRLADQDVA"
     gene            complement(16576..16911)
                     /locus_tag="VE646_01215"
     CDS             complement(16576..16911)
                     /locus_tag="VE646_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZD78646.1"
                     /translation="MEVRRLQVPDSPSRRFDHGEVLVRQGERSAWLDLVREGAVALTS
                     VLPSGRAVVVGLLGAGDLFGEAALAGSDASPVEARAVGAATVSALPVEGPTRSWRATR
                     RPRWRSSGC"
     gene            17039..17461
                     /locus_tag="VE646_01220"
     CDS             17039..17461
                     /locus_tag="VE646_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZD78647.1"
                     /translation="MGKREQQALAQVPLFSPLSARYRRRLADHLEEVRYMQGAPIVKE
                     GESGDTFFVILEGQAKVQNPKGRVVNRLVPGDFFGEISLLDGGARTASVVTETPMTLL
                     ALKRGAFIKLLEAEPAVAVKLLEYAASLLRRMQRSLSA"
     gene            17483..17848
                     /locus_tag="VE646_01225"
     CDS             17483..17848
                     /locus_tag="VE646_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78648.1"
                     /translation="MRVELVRPDDAETVVGVIRWDGRRGHLEPMADGVQGLEDILRPT
                     PVVLDDPSFRRAGTHGPTVLQPGDYDWFRAAVITRAEALGLEARFVPDGIGYGWDPAA
                     TYRTFEHQVERITTPSREE"
     gene            complement(17854..21120)
                     /locus_tag="VE646_01230"
     CDS             complement(17854..21120)
                     /locus_tag="VE646_01230"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HZD78649.1"
                     /translation="MMAGYPGPILDFLGAEPTPEQWTAISMPLVPYVLVAGAGSGKTS
                     VMAARVIYLAMVANGDIQADHPGVMPGNVLCLTFTNKATEHLLLRIRAALRMLRLDEG
                     EEPEILNYHGFAAQILDRHGMLIGIEPGQRVITPAQRAELAGRVLDLMTFPDLQTRWQ
                     PSIVRSILSLDEQLQNHRVEPEEVRRFVRANLERLQAARSADPYRAALERLQLADGVS
                     AFRELKRRLGVIDFGDQIGHAVRIVEDHPEVVQAYRDRFQAVLLDEYQDTNHAQALLM
                     EAVFGRGHPVTAVGDPDQNIYGWRGASLHNLLRFPTQFPSADRSPSPRLPLYMNFRSG
                     GRILEAAGTVIRPLPLEQRPDPGKELEPYPPNGEGDVWVDQYEHEVAEAEAIVDRIQE
                     LHGTGVAWKDVAILCRTHRLFEPLQLAFQDRGVPAEFIGLAGLIRLPEVVEILAYARA
                     AADPAASVALARILTGPRYRVGLADLARVAAWTRSSGWSFIDRLADRMPEDEDLLEDQ
                     PFLIAEALEHLDEVEGLSPEGRERLEAFAGELADLRGAARRPVGEFLAEVIRRSGLLA
                     ELDAHPDRALAGARTRNLSAFLDQVHAFQPLEGELTLRAFLDYVDSIEDDREWTPVQP
                     SDDDSVKVMTVHAAKGLEFDTVFVPGLAGGLFPNTRVQQNPMRRGSSLDVELRRDRDL
                     LPAFDGVMSHFVAALRDQEEFEERRTAYVALTRAKRRLFCSSALWYGESLRAKGVGLF
                     FGELAKWARGEPGVRVSFQGGEEDGANPLAGYRQRFVRSWPGPARPDESDPLFPDGWR
                     RAALHAVRTGAIPQAALSELSPEERARYETLAGERRTLAVHLRERESMTAVPPPAPTA
                     VSVSGLIDYGRCPKRFYWTTIRPLPRFSGPAARIGTDVHAWIERRSSGQASLLELEEP
                     PDLVAEELAGEPGKVQQLREAFLTSRFADAVPLFAERPFLLSIDGAVVNGRIDAIFGT
                     PEGAWEVVDYKTGRRPSSDDPSSQIQLDLYALACIDVWRKPAEDLTLTYLYLSTGEEV
                     SRPAGDPGAIRDRVRTWLAGIQGGRFEPTPGEHCRWCDFLSFCEPGTAFVERRSSPAP
                     "
BASE COUNT         3133 a         7371 c         7561 g         3074 t
ORIGIN      
        1 acgccttcga cgcgcgggcg ttcaggtccg cgatggcgcg ctgggcggtg gcctgcgcgg
       61 tcgccttctg ccggcgcacg tccgcgagct tggactggat cgacgccagc ctgatccgct
      121 cttggttgat ctgctcatcg agctgggaca ggtgctggtt cagcgcatcg agcttcgcct
      181 tggccgcctc cacgtcctgc ttcgacgggg aggcgaagga ggtgcccgag agggcgaggg
      241 aggtggagag gacgagcagc gaggccaagg tggccccgac gatccgccta ccgttcccag
      301 tcgccctcgt catccgaaga gccggaccgc tcatcgctgg ccggcgcccc agggtaccgg
      361 cgggatcggg ggtcgtcaaa caccccatcg ccttgtaagg caacgggcgg ggccccgcat
      421 ggtgtcacgg agggtccgcc gaacggtggt cgggacggcg ggatttgaac ccgcgacctc
      481 agcgtcccga acgctgcgcg ctaccaaact gcgccacgtc ccgaggcccg ccagtctacc
      541 ggctccccgg accggtgggc ggtagattgg gaaccgagag cgcgctggga gcgtcacgtg
      601 atgccgcggt ggaggagggt gagagccaca tgaacgggtc ggccgtgcgt gtcgagtccg
      661 acagcatggg caccatcgag gtgcccgccg atcgctactg gggtgcccag acgcagcgtt
      721 cgctccaaca cttctcgatc ggcgaggacc ggatgcccct ggaggtggtg cgcgccttcg
      781 gcatcctgaa gaaggcctgc gccctcgtga accgagatct cggcaagctc gcggccgaca
      841 aggccgatct catcgtgagg gccgccgacg aggtgatcga cggcacgctc aacgagcact
      901 tcccgctgtt cgtgtggcag acgggctctg gcacccagtc gaacatgaac gcgaacgagg
      961 tcatctccaa ccgggccatc gagctcgccg gtggagagct gggatcgaag accccggtac
     1021 atccgaacga cgacgtgaac atgtcgcaat cgtccaacga cacgttcccc accgcgatgc
     1081 acgtggcggc cgccacagcc gtggtccagc gtctcgtgcc ggccgtggcg cggttgaggg
     1141 atgcgctgca gcgcaagagc gaggagttcg cagacatcgt caagatcggc cggacccacc
     1201 tgcaggacgc ggttcccctg acgctggggc aggagttctc cggctacgtc gcccagctgg
     1261 acgcggacat cgcccggctc gagcaggcgc tcccggcggt ggaggagctc gcgatcgggg
     1321 gcacggcggt ggggaccggc ctcaacgccc ccgccggctt cggcgagcgg gtcgcggcga
     1381 agatctccga gatgaccggc ctgccgttca cgggcgcccc gaacaccttc gcggcgctgg
     1441 ccgcccatga cgcgatggtc acgatccacg gtgccatccg cacgctcgcc gtctcgctga
     1501 tgaagatcgc gaacgacatc cgttggctgg gcagcggacc tcgcagcggc ctgggcgagc
     1561 tcgagctccc ggagaacgag cccggttcgt cgatcatgcc cggtaaggtc aaccccaccc
     1621 agtcggaagc catgaccatg gtgtgttgtc aggtcttcgg caacgatgtg gcggtcaccg
     1681 tggccgggtc ccagggcaac ttcgagctga acgtcttcaa gccggtgatg atccgaaacg
     1741 ttctgcattc ggtcgcgatc gtctcggacg tctgccacac gttccgggag ttctgcgtgg
     1801 aggggatccg cccaaaccga ccccggatcg cccggctggt gaacgactcg ctcatgctgg
     1861 tgaccgccct gtcccccaag atcggatacg acaaggcggc cgagatcgcc aagaaggccc
     1921 accagggagg gaccagcctg aaggaagcgg cgctggccct cggctacctc accgagcggg
     1981 agtacgacga gacggtccga ccagagcgga tggtcggccc cgactaggcc taggcctgac
     2041 cgccgctgcc gctcggcgcg ggtcgcagtt ccagcagcgc cgcctcgggt cggcagaaga
     2101 accggaacgg cgcgtacttg ctggtgccga gccccggaga gatgtggacc caggtgttcc
     2161 cggcccggat caaccccgag acgatgcgtc gcggcatgtg cgagttcgtc accagcgccc
     2221 ccaccagcgg cagtcggacc tggccgccgt gagtgtgacc gaacaccgcc aggtcccacc
     2281 ccagggccga gagttcgggc agggggtcgg gtgagtggac caccgcgagc ccgaaccggt
     2341 ccgggcaggc tcgcggcgcg gcgcgcaggt cctgacgccc gatgtgcgga tcgtccaggc
     2401 cgagcacctc cacctccagc ccgtccagcg tcgcctcgat ccgccggttc ctcaggtgga
     2461 tccagccgtc ggcctccagc agacggacca ggtcgtcggc ccgcccccgc cgcgcccgcc
     2521 gccggggccg gtgcttgagg aagtaccgca ggtagttcag gggtcggggg gcgtagtagt
     2581 cgttggagcc caggacgacg tacgaggcga tccgtccgcg gaccggccgc aaggcctcga
     2641 ccaccgcctc cacggcctca ggctcaccca ccaggtcccc cgtgattgcc gtgacgtcgg
     2701 caccgggcag gcgggacagg aaccggcgca tggcggccgc cctccgggtg agatgcagat
     2761 ccgagaggtg gagcaccgtg agtccggccg aaccacccct cggtaggatg tccagtcggt
     2821 agcgacggag ccggaaccat cggcgctcga tgaagatgcc gtacaggacg cacaggatgc
     2881 cggcgaacac gatcgcgagg agcacccgga tgagcacggc acggagtgta cggtgagcga
     2941 tccctcgctc aaggaccgga tccggcagga catgacggcc gccatgaagg ccggcgatcg
     3001 tctccggctg ggggcgctcc ggatgctgtc cacggcgatc acgaaccgcg agaaggaggt
     3061 gctccacgag ctctcggacg acgaggtgcg gcaggtcgcc gcgcgggagg tgaagcgccg
     3121 caacgagtcg atcgaggcct tccagaaggg tggccgtgcg gacctggtcg agaaggagag
     3181 tgccgaacgc gagatgctcg ccccctacgc tcccgagcag ctctcggagc aggagctgga
     3241 cgctctgatc gacgaggtcg tcgaggcgac cggggcgagc ggcccgaggg atcttggcaa
     3301 ggtcatgggt caggtgatgg gaagggccaa gggtcgggtc gacggctctc gcgtgcaggc
     3361 caaggtgcgc gggcggctgg gcgcttaggg gtccccgtcc cggccggtcg gcgccggacg
     3421 tgctcacccg ctgggtgcgg gcgtgggggg tggggtcggt cccggggagg ccttcgacct
     3481 gccaacctcc agaaccacgg tggagcccgt tgcagcttgc ttgccaccga ccggcgtctg
     3541 tgccaggacg agcccgtcct gcttcggatc ctcgacgtcg acgaaccgga tctgcaccac
     3601 cagccccgtc tcctccacgg ccttgaccgc ctgatcccga tccatgccca ccacccgcgg
     3661 gacctcgatc aggtccccct tgccgttgct gacctggagc ttcaccccgc cccccaaggc
     3721 caggctggcg ccggcccggg gggtctgcgc gatcacggtg ttcaggggga gcgacgaatc
     3781 gatcttctcg atgatggggg tgaagttggc gtcggcgagc ttcttctgtg cctcgagcga
     3841 gcgcaggcca acgacccggg ggaccttgcc gtactggggc ggtggtggcc cggggaagga
     3901 tgccaccggc atcccggcca aggccttgac catgaagtcg tgccagatcg gcgccgccag
     3961 cgtgccgccg aagacggagt gcccgtagta gccggacatg gggacgttgc cctgtgggaa
     4021 ccctacccac acggccgtgg ccacctgggg cgtgaacccc gcgaagtaca cgttcgtgta
     4081 gtcttgcccg gtgccggtct tgcccgccac cgggcggccg atctgcgcgg ccgtcccggt
     4141 gccgcagcag accacacgct gcatcatcgc tgtcaccaag tgggcgatgt ccgggtcgat
     4201 cgcctgcttg cactgccggt ggtgctggta gagcttcttc ccctctggca gcaggacctt
     4261 ggagatcgcg tagggctggc agtggacgcc gccgttggcc agggtcgagt acgcggaagc
     4321 catatccagt gtggagacct cctcggtccc gagcgtgatg gaggggacgg cctggagcgg
     4381 cgtggtgatg cccatctggt gagcggcgga gacgatgttc tccggtccca cgtcgagcgc
     4441 gagctgcgcg aagacgacgt tgatcgaatc ttcggtggct cgccacaggt cgatgtagcc
     4501 ctcgttcccc ggaccctcag cgttcgagac gcagtggtcg gcactccgcc aggagggtga
     4561 gcagtacggt gacttggagg ggtagacctt gccggccggg aagccgtgtt cgaacgccgc
     4621 caccagcgtg aacggtttga acgccgagcc cacctgccgc atcccgtgcc aggccagatc
     4681 cagctggtcc cgtcggtagt ccttcccgga cagcatggcg cgaatcgcac cggtcctcgc
     4741 gtccaccgag accagcgagg tgtcgggtcc tcggtccatg tcgatgttcg ggctgcggtc
     4801 cacggcctcc tgcgcgtacc gctgccagtc cggatccagg gtcgtgtaga tccgcagccc
     4861 accctggaac aacgtatgac ggcgctgctc gtacgtcttg ccgaacgcgt cgaaccgtcc
     4921 gtccgcgtcc tccaggatcg actgcacgat gtagtggacg aagaacggtt ggaccttctg
     4981 gccggtcgtc ccggcggtct tcgcgaggcg gatcgtcgag gcgatcgctg cgtccaccct
     5041 ggcctgcggg acccatccca gagcccccag gcgcgtgagg acctcgttgc gtcgccggag
     5101 cgccgcctgt gggtgcttga ccggatcgta gtcgccaggc gcctggatca atccggccag
     5161 cagggccgcc tggggcaggt tcaaggccga ggccggcttg ccgaagtagg tctccgcggc
     5221 cgtcccgatc ccccaggcgc cgttgccgaa gtagacctcg ttcaggtaca gggcgaggat
     5281 ctgatccttc gagtacttct gctccagccg gatcgccagc gcggcctcct ggaacttccg
     5341 ggcgaaggtc tgctccgtgt cctggatcac ggcgttcttg accagctgct gggtgatcgt
     5401 ggatcctccc tgggtgatcc ttcccgcgat caggttcgcc aagagcgcgc ggaagaccga
     5461 gggcacgttc agcgccccgt gctggtagaa cccgtcgtcc tcgatggcca gcaccgcttt
     5521 ccgcgcgatc ggggcgacct gcttgaggcg cacgatctcc cggttctcgt ccaggtacaa
     5581 ggtcgcaagc acagagccat ccgcggcgta gatcgtggag cgttccggga agtgagggac
     5641 cctggtgaac gaggccccgg cctggtccag gcgggcgttc agggcatcgg cacccatgct
     5701 gaccacaccg gcgatggggg agagggtcac ggccacgagc gctgacgcca ggccgatcac
     5761 gaggaacagg aggatcgggt acagggggtc cttcggcagg cgtaccgtcc gccgtgccgg
     5821 ggcctcgacc cggcgggagc gtgcgcgcga cgaagcttgc atgtggaggt cggcccaagg
     5881 atactcccgt ccctcccggt accatgggag ccgctcatgc gccgcctcac cgccctgttc
     5941 ccgctcctcc tactcgccct cgccctggcc atcccggccg cgcaggcgca gtccgaggaa
     6001 cgagctccgg tcatcgtggc caagatcgac ggctcgatcg atcgcaccct ggccgggttc
     6061 ctgacggaca cgctggacca ggcgcagcgg accggtgcca cggtcgtcct cgagctggac
     6121 agcgcgggga cgctggaccg ggatggcatc gatctggcgc gacggatctt ccgctcacgg
     6181 gtgccggtga tcgtctgggt gggccccacg ccggccaagg cacagggggc cgcgctgctg
     6241 ttcctgtacg ccgcggcgct gccggccgtg gctccggggg ccggcatcgg gcctctggag
     6301 ccgctcgacc tggtcgatgc ccggcaggcg accatgcccg atccggccga ggtgcgccgg
     6361 agggcggctg cgtgggccgc ggcccgtggc cgggggaacg tctcgttccc ggccacgccg
     6421 atcccggcac agcgggcgat cgatgagagg gtcgcggcgg tcgccgcacc ctcggtgacc
     6481 gatctgctcg accggatcga cggcaccacc gtgaccacgg aggcgggcga ggtcaccctg
     6541 cacaccaaga tcgccacgga ggccggccag gcgaccgtgg acgtccgatt cacggatccg
     6601 gggcccgtgg accgcgtcct ccacgccatg gcctcgccgg cggcgatcta tgtgctcctg
     6661 gtgttcgggt tcgccgcgct ggccttcgag ctcacgcagc ccgggttcgg cttcgccggg
     6721 ttcagtggtc tggccatgat ggccctggcc gcctacgggc tcaccgcggt tcccgtctcc
     6781 tggctgggcc tcggcttgat cgttgcgggg gtggtgctgc tggaagcaga tgtcctcctc
     6841 cgcaagctgg gacccctcag cgggctgggg gtaacggcgt tcgccggtgg atcgctgctc
     6901 gctttccggg cggtggcccc ggccatccgg ctctccccct ggctgatcgg ttcgctctcg
     6961 gcggccagcc tcctgtacta cgggttcgcg ctgaccgtcg ccctgcagtc gcgggatcgg
     7021 atcacgtcct cccagaaggg cctggtgggg ctggtcggcg aggcgcgggg ggacctggag
     7081 ccggagggtc cggtcttcgt caaggggacc ctatggcggg gtcgcagcgc cgatgggccc
     7141 atcccctctg gggcccgggt gcgggtccgg ggtgtcgacg gcctgatcct gcgggtcgag
     7201 gcggaccccg atccggcagg gtcgccaccg gagggctggc ccgaggatcc cggaagcgcc
     7261 ctgccgtaga cctcgaagat gtgcgttttc cgcgacatct cgcctggata cccgcttgtc
     7321 ggagatcccc agggcggcgc tgtacgaagg acatggatga gggatcacga aggaggaagc
     7381 ggcgacggcc gcccgcgaga tgtcggtgcg atggacctgg gcgtggcagc tcgaggctgc
     7441 ctgccgaggc gaggacgcct cgctcttctt cgcgccgaac tacttcgaga agcgtgagga
     7501 caagctcgct cgagaggcga aggcgaagcg ttcctgcgcc cggtgccggg tccgggacga
     7561 gtgcctcgag tacgccctca ggacccgcga gccacacggc atctggggtg ggttgaacga
     7621 gttcgagcgg cgtcaggtcc ttcgagagcg cgcccttcga gccggctgag gatcgcgaag
     7681 cacttccctg ggggcggcgt catcgtgcgc cgccccgttc gcgtctgaca ccatggttcc
     7741 gcatccaaga gcgggaggag gggtcccgtg gtcgagaaca tcgaggaccg tctggcatcg
     7801 ctgaagatcg agctgccacc ggctccaccc cccgtggcct cgtacgtgcc ggtccgcatc
     7861 gtcggggacc tggcccacct gtcggggcag atcgccatgg tcgacgggga gccgatccac
     7921 cgagggcggg tgggtgggga tgtgtccttg gagcagggcc aggaggccgc ccgccgatgc
     7981 gtgctccagg cgctcgcggc gctccgggat gtggcagggt cgctggaccg ggtggaggcg
     8041 atcgtccagc tgagcgtgtt cgtggcctcc gaggacgggt tcaacgatca acccaaggtg
     8101 gccaacggag ccagcgatct actggtcgag atcttcggag acgccgggcg acatgctcgc
     8161 gtggcggtgg gcgtgggcga gcttccgctc ggggcaccgg tggaggtcgg catggtcgtc
     8221 cacctcatgc cgcgatgacc tccgcgccgg cggaggcgct caggcctccg gccgggggaa
     8281 ccggaggtca ccccgacgga tcgtcacccc gcgttggtcg aagcgctcga gcagcggtac
     8341 cgcgaacttg cgggaggtcc ccagccgtgc tcggaacgtg ctcacggtga ggccgtccgt
     8401 cgcctgccgg accacctcct cggcccgctc gatgaaggcc ggtgtcatga tcagctgggc
     8461 gccgatgcgg accagcgcgt gggcccgagc cgcggcctcg atcacctctc gggagaaccc
     8521 caccgcggtg agctcggaga tggtcggggg cgtcggctcc cccgcagcca ccgcgtcgac
     8581 cagcctcctg acctcgtcct cgtgcccgga gaggctgacc ctgtgcgcgg ccaggcgcac
     8641 catcctgccc tcgcgcacca ccacgccccg aacatccaga tcgtcgagca gggcctccac
     8701 cagcgctgag gccggtgccc ggccggctcc gcgcagggcg gcggcgacgg acgatcggac
     8761 cccgacgagg tcctggcctt gctgcagcgg gttggcgtcg tggaaggcgc ggagaccctc
     8821 gtgcacggcc cgctccacgg cagaggcgag ggaaccggcg acccaccatc caccgatcag
     8881 ccgggcgtca ggcatggcct cgggaacagg tccggccagg gccgccaggt ccgcgaccgg
     8941 caccgccccg cgctcctgca cgaccagccc gggtagctcc ccgcgggagg cgccctcgcg
     9001 agccgagagg cggtcctgag gcgagggccc gggccgcccg ggagggtcca cgtccaggac
     9061 gaccccgccc gccacggtct ccctgcggcc cgactcgcga agcacgaacc ggtcgtggac
     9121 gtcgagcacc agcggtgccg acagccggat ccgcgcgaac gctcccggcg agccggcgtc
     9181 gagctccccc gatccgtaca ggcggatgcg ggcgtcgcgc tccgccgagc ccgcgtagat
     9241 cttgtaggcg ccgcggttcg tgatcgggtg gaccaggccg cgcaccggtc ggagccgggc
     9301 ctcgatcgag gtggtggaac gccactgccc cggcaggccc aggacgtcgc cgcgctcgac
     9361 ccggtcgcgc tcggccccgg ccaggttggc ggccacccgg gagatcggcc gggcacggtc
     9421 gatcgaacgc ttgtgggtct gcaggctccg gatccgagcc cggatgccga cggggtacag
     9481 ctcgacctcc tggcccacct cgagcgcgcc cccggtcagg gtgcccgtca ccaccgtccc
     9541 ggcgcccttg atcgtgaaca cccggtccac gaactcgcgt gcccgacccc ggtcctcggc
     9601 ggggggtgcg gcgccgacca tcgcgtcgag cgccgagatc aggtcgtcca ccccgtcgcc
     9661 ggtcaccgac gaacagggca cgacgggggc accctccagc acggtcccct ccagccgttc
     9721 gcggatctct cgctcgcgca ccgccaactc gtcgtcgtcc accaggtcgc gcttggtgag
     9781 ggccaccacg cccccctggg cgcccagcac gtcgacgatc gccaggtgct cctcggactg
     9841 cggcttccag ccctcgtccg ccgccaccac gaagagcacc agccgtaccg atccgacccc
     9901 ggccagcatg ttgcggacga agcgctcgtg ccccgggacg tccacgaacc cgatctctcg
     9961 tccggagggg agcgtgaacc aggcgaagcc gaggtcgatc gtgaggcctc ggcgcttctc
    10021 ctcctcccag cggtccgggt cgatgccggt cagccgaacg atcaggctcg atttgccgtg
    10081 gtcgacgtgt cccgcggtcg ccaccacgtg cagcggcgac ccagggctcg ccccgccctg
    10141 gtcgaggccc gcccccctct ccgggtgggc cgcgggctcc ccgtcacgcg tcatctggga
    10201 gctcgtcacc ctccagtgcg tacccgacgg cgcgggccag gtcggccgtt cgctcttcgg
    10261 cgacggtccg cagatcgaac agcaccccgt cctccgccgt ccggcagaag acggggggcc
    10321 ggccggtgcg cagccgagcg gcgaacgccg gagggtgggg gaccttcagg tggacacccc
    10381 acgagcgaac cgtgaagccg ggcatcgagc caccccccac ggccgaccca cactccacca
    10441 cgtgggcgcc ctccagatcg ccgtccagcg ccagcgacag gcgttcggcg cgagccttca
    10501 ccgtgctcgc cgcctcttgc agcatccgcc agatgggaag gtgatcccgc tcgccccggg
    10561 cgtaggcggc caggacggcc tcgagggccg ccgtttgcat cttgtccacc cgcaacggcc
    10621 gggcgatcgg atgtcggcgc atccgcgtca ccagctcctc ggtcccgagg acgatcccgg
    10681 cctgcggtcc acccatcagc ttgtcgccgg agaagatcac caggtcggca ccgtcgcgga
    10741 gcgcttcgtg ggcggacggc tcgtcgtcgg gcacgccggg gtaccggtgg agcaggcctg
    10801 agcccaggtc gaagaggaag ggcagagctc gctggtgggc cagcgaggcc aggctggcgg
    10861 tggccggcgc cgccgtgaat cccaccaccc ggtagttgct ggggtggacc ttcagcaccg
    10921 cggcggtccg gtcgcccagg gcggcccggt agtcgctcag gcgggtgcgg ttcgtcgtgc
    10981 cgacctcgac cagcttggct ccggaggccg ccatgatgtc cgggatccgg aactcgccac
    11041 cgatctcgat cagctcgccc ctcgagacca ggacctcctt gcgccgggcc agcgcggaca
    11101 gcgccaggag cagggcggcc gcgccgttgt tcaccaccag cgccgcctcg gcaccggtga
    11161 gcgcggtgag cagggtctcg gcgcgggcgg agcgcgtccc gcgcttgccg gaggcgcggt
    11221 ccacctccag gtcgctgtag ccccctgccg tccgccgcac cgcctccacc gcctgctccg
    11281 ggagcggggc ccgacccaat ccggtgtgca ggaccacgcc ggtggcgttg atcacctcgg
    11341 tcagcccggc ggccgtgtag gagacgcgct ccagcgctcg ggccaagatg acgtcggtgt
    11401 cggggggagg gctcccgccc tcggcgcgct ctcggacctc gtcgatggtc tgggcgacgg
    11461 cccgcttgac cagggcgcgt ccgaaggtcg ccgatgcgcg gttgccgggc tccgagcgca
    11521 ggagggcatc caccgacggg atgctgcgtc gaagccccgg cgtggcgccc atcgcccgcg
    11581 agcctactcg ctcgacggtc cggtgtgcgc cgtgggcggc gtgcccgtct catgggagga
    11641 taggagaccg tggatacgag ggaacgagcc gaggaacggg tcgcgttctc caccgccgcc
    11701 gagcactgga tggtgcgcgg cggcgggatc gatgtcgagg tggtctcccc gaacggcctg
    11761 cgcaccgagg agatgatcct caacatcggc ccgcagcacc cttccacgca cggggtgctc
    11821 cgggtggtcc tcgagctcga cggcgaggtg atcacccggg ccgagccggt gatcggctac
    11881 atgcaccggg cggccgagaa gctatccgag caccgcgacg cacgccaggt cctcgtgctg
    11941 atgaaccgtc acgactggct ctccgcgttc aacaacgagc tcgggtgggt cctggccgtc
    12001 gagcggctgg ccggcattga cgtcccggag cgggcccggt ggatccgcac gatgatggcc
    12061 gagtggaacc gcatcctgaa ccacctgatg ttcacgggct ccttcccgct cgagctcggg
    12121 gccatgacgc cgatgttcta cgcgttccgc gagcgcgaga tgatccagca cctcatggag
    12181 tctgccacgg gtgctcggat gcaccactcg tacgctcggg tgggcgggct gaaggacgat
    12241 ctgcctcgcg ggttcctgaa gcagtcggcc gaggtcctga agtgggtgcg caagaagctc
    12301 agggacttcg accagctgat catggggaac gagatcatct tcgctcgcac caagggcgtg
    12361 ggggtcatcc cgccggagat ggcgctggcg tacgggtgct ccggacccgt gctgcagggc
    12421 tcggggatcg gcatggaccc ccgcaaggac gagccatacg agaagtacgg cgaggtggag
    12481 ttcgacgtgc cggtgggcga gcacggcgac gcctacgacc gtctgtgggt cttggtccaa
    12541 cggatgtggg agtcctgccg gatcatcgag cagtgcatgg accggctccc acccggtccg
    12601 tacatagctc ccaagctccc caagaagctg aacctggacg gtgagatcta cgtccgtacg
    12661 gagaacccgc tcggcctgat gggctactac ctggtcggcg acggcaagaa gtacccctac
    12721 aggctgaaga tgcggacggc ctcgttctcc aacgtgtcgg tgctcgcggc catgctgccg
    12781 gggacgctgg tgcccgatct catcgcggtg ctgggctcga tcttcttcgt ggtgggcgac
    12841 gtcgatcgct gatccggccg cgatccccct cgggaagcac ggtcttgctc aacgctggcg
    12901 gccgcgtcga agctctgacc gcaggtgacc aggtggcccc gagatcggtg cctcggacac
    12961 catgacatgg ggcttccatc tgcccagccg ggggcggaca tgtggacgag ccgtccgatg
    13021 gcgcccccgt cgcatgacgc tccgatccgt tcggtcgcga gcgggttcga tcgacggatg
    13081 atgaggttga acggtgggtc aatcggccga agggcgggac cgccgttccg agccggcgcc
    13141 gagccaggac ggggcgggca ggccagggtt cgacagcagg agccagcgca aacgccccag
    13201 cccagatggg tccaccaaca ggctcgctcg gctccggccc tgccagatcg ccaccgagcg
    13261 cccgcccgat ccctctcgga tcgacgcgac ctgccgctgc agctggccct cgttccaggc
    13321 tcctaacccg agcgccatca gcgcgtccgt ctgggagacc tccccgagga ggatgaggcc
    13381 ccgatcggcg gcgcgctccg tcagcgcgcc gaagtccacg ccggcggtga tgtcggcgct
    13441 tcccggatcg gcgaggacgt cctccaccac agcgtgggcg cgatagccgt ggacctcgcc
    13501 gatcgggtgg gacgacgcgc cgtagtcgat cagcagcgcg tatcccgtga ccagggcatc
    13561 tgccagctcg tccatgaaca tcagggcgtc ctggggcacg gctgcttcct cgcccggcgc
    13621 gagggccggc aggtgagggg ccagctcatc gtcgcctgga cccgtgacct ccaggaaccg
    13681 ctcgtccgtg gcgtccacca gcacctcgac cggaccccgc tccgtcccac ggacccggcg
    13741 gaacggcagg ttgtccagga gctcgttcgc gaacaccagc ccgggatcga ggggtgggag
    13801 ctcgtcgagc cgctcggcga cgtggatccc cagcgccccc agggctcgac gcgcacccga
    13861 gctccgctcc accgcccaga gctcgatcgc aggaccgacg tcggtggaga gtcggaggag
    13921 gtcgcggcac agggtgccgt cccccgctcc gacctcgacc acgcgcaggg ggtcgggacg
    13981 accgagcccc tcccacagct cccgcaggcc gcggagcagc agctcggcga agaccgggtg
    14041 cacgtggggt gcggtgacga agtgcccttc ggcacccacc ggaggacgct cgtagaaccc
    14101 gccgcgcccg tagagcgcga tatccatgta ctccgcgaac gtgatcggtc cgtgagcggc
    14161 gatcgcctct cggacccggg acgccacttc ctcgctcacc gccccagggt agatgctcgg
    14221 cgatcggccc ttggtccccg ggcccccgcc ctcgccctcg ccggatccga gccggcgccc
    14281 ggccgcgccg ggctgggaga atgcttcgca tggagggtgc gatccgcaag gcggcatccc
    14341 tgatcgcggt tcggcgggcc ggcgaagggc ccgaggtgct cgtggtcgag aggggggcgc
    14401 agagccgatt cctccccggc tacgtggcct tcccgggagg ggctaccgac gtcggcgatg
    14461 ccgacctggc cacgcggtgg ttcggctcgt cggtggaggc accgagggca tgcgccgtcc
    14521 gcgagctggc ggaggaggtc ggtctggtcc tcacggccca gggccttcga gcggggacga
    14581 ggggcaccct cgatgacccg gctgcgtccc ccccgacggt ggatcagctc ccccggatcg
    14641 cccactgggt ggcgccggag gacgtgcccg tccgcttcga cgccgactac ttcgccgtgg
    14701 cggcggaagc ggggatcgaa ccggagccgg atggcgggga ggtcgcccgg gcatggtggg
    14761 catccccggg tcggttgctc gccgaatggg cggagggcac gcgccgcctc tactggccga
    14821 cgtacttcac gctccagcac ctggcggcgt gcgctcggat ggaggacctc ctggctctgc
    14881 ggatccccac ccgagaggct gatgaccacg aggtcgcccg gctgccgcgc tccatcttct
    14941 ggcaggacga gggctgagcg gtgcgcgtcg tccgggtcct ggcgccgaac cccggcctgc
    15001 gcgagctgga tgggacgaac acctgggtgg tgggtcggtg gccggccgcc gtgatcgatc
    15061 cgggaccgga cgacgcgggc cacctgaccg aggtggtgcg ggcagcgggt cgtgtctcgc
    15121 tcgtcctcct gacgcacgac catcccgatc acgcccccgg ggcggcgccg ctggcggcca
    15181 tcaccggggc gcccgtgctc tcggcgcggg gcgaagaagg cgggcatctg cgcgacggtg
    15241 aacggctgac gatgcccggg gtccggctcc aggtcgtggc cgccccggga cactcgccgg
    15301 accatgtggt gttcttcggt cccgaggacg gagctctgtt caccggggat gccgtgctgg
    15361 ggcgtggcac gtcgctcatc gacccgcccg agggcgatct ggttgcctac ctgcgatcgc
    15421 tgcaccggat gcgggacctc catccgcgca cgatccatcc gggtcacggg ccggtcgtcc
    15481 tggacgcggt cggcaagctc gaccagtacc tgcaccaccg ggcggagcgg gaggagcagg
    15541 tcgtggcggc cctccgggcc gggatgagga ccccggagga gatcgtcccg acgatctacg
    15601 gggatcatcc gcaggagctc cacgagttgg ccgcgaggag cgtgctggcg catctgctca
    15661 agctggagac cgagggtcga gcagccaagc ggcttcgcga cggcatcccg cgttggcggc
    15721 tgcaggagcc gcggacctgc gaacgatgcg ggcgcccggt ccagggtcgg gggcggctgt
    15781 gcccctcgtg cagcatggcc gtcctgcagg aagaggccta aggggcgctt tccctctccc
    15841 accccgagac atgcttgccc tcgcctgtgt acggctcgcg cttccatccg ccccatcgct
    15901 cgcggagccg cagaccggcc agccgagcgg tcgagctcgg aaggccaggc gtaccggcga
    15961 ccgggatgcc cgctccgccg ggcttgcccc tcatccgctc gaccatctgc tcggacgcgt
    16021 cgatcctttg gaccgccacc acggccgccg ccgggggcgc gacccgaccg gtgccgatgc
    16081 cgagctcgag cgcaggaccc gaggcggcga gcccttcgag gaaggtcacg gtgtcctcga
    16141 cgggccccga ggagggcacg gctccgtacc gcgggtcgta gaagtcggcg atccgatcgc
    16201 cgtacgtaga tgcctcgtat ccgtccatgc gcgagcctgg acgggggggc cctcgtcctg
    16261 tcaagacgtc ctcaggccac gtcctggtcc gccaggcggg tgagggccgg gagatccgcg
    16321 atcacgaacc gccgatcctg ggtgcggacc aggccctgtc tggacagggt gaccaggctg
    16381 cggttcacgg cctcccgaga ggcacccacc atgcgcccga ggtgttcctg cgtgaggggc
    16441 acggtgagct ggaccccggg ccgaacggcg tgccgtgccg acgcgccagt tcgcacaggc
    16501 tgcggcacag gcgagccgag aggtcccgcc ccagcgcgtc ctccagcgcc gtcgcggtgg
    16561 ggtgcaggcg gaacgtcagc agccggagga cctccaccgc ggtcgccggg ttgcgcgcca
    16621 ggacctcgtg ggcccctcga cgggtagggc gctcacggtc gcagctccca ccgctcgcgc
    16681 ctccaccggc gaggcatcgc tccccgccag ggccgcctca ccgaacagat cgccggcacc
    16741 gagcagcccc acgaccaccg ctcgccccga cggcaggacc gaggtcaggg caacggcacc
    16801 ttctcgcacg aggtcgagcc acgccgagcg ctcgccctgg cgaacgagga cctcgccatg
    16861 atcgaaccgg cgggaggggg agtccgggac ctggagccga cggacctcca cgggtctgga
    16921 gcctatcgtc cgccaccgac atccgttccg gcgcatcggc gaggggaggc cgaccttcga
    16981 cagggtgggt cgcacgaggt aggctccggg tcgaccgccc gaggccgagg agggtgcggt
    17041 gggcaagcgg gagcagcagg cgctggcgca ggttccgttg ttctcaccgc tgtcggcccg
    17101 ctaccggcgc aggctcgcgg accacctgga ggaggtccgg tacatgcagg gggcgcccat
    17161 cgtgaaggaa ggcgagtcgg gcgacacctt cttcgtgatc ctggagggac aggccaaggt
    17221 ccagaacccg aaggggcggg tggtgaaccg tctggtgccg ggcgatttct tcggtgagat
    17281 ctcgctgctg gatgggggag cgcggacggc gagcgtggtg accgagaccc ccatgacgct
    17341 gttggccctg aagcggggcg cgttcatcaa gctcctggag gccgagccgg ccgtggcggt
    17401 gaagctgctc gagtacgcgg cctccctgct gcgccggatg cagcggtccc tctccgcctg
    17461 agcccggtac gctgggcgca gcatgcgcgt tgaactggtc cgcccggatg atgccgagac
    17521 ggtcgtgggc gtgatccggt gggatgggcg gcgagggcac ctggagccca tggccgacgg
    17581 ggtccagggc ctcgaggaca tcctgcgtcc caccccggtg gtgctggatg acccctcctt
    17641 ccgcagggcc ggcacgcacg gccccacggt gctccagccg ggcgattacg actggttccg
    17701 ggcagcggtg atcacgcggg ccgaggccct cgggctcgag gcacggttcg tgccagacgg
    17761 catcggatac gggtgggacc cggccgcaac ctaccggacc ttcgagcacc aggtcgagcg
    17821 gatcaccacg ccgtcccgcg aggagtgacc tccctacgga gcaggggagg atcgccgttc
    17881 cacgaacgcc gttccgggct cgcagaacga gaggaagtcg caccagcggc agtgctcgcc
    17941 cggcgtcggc tcgaaccgtc cgccctggat gccggccagc caggtcctga cccgatccct
    18001 gatcgccccc ggatcgccgg ccggacggct cacctcctcg ccggtggaca gatacaggta
    18061 cgtgagggtg aggtcctcgg ccggcttgcg ccagacgtcg atgcaggcca gtgcgtacag
    18121 gtcgagctgg atctgggacg aggggtcgtc gctcgatggc cggcggccgg tcttgtagtc
    18181 gacgacctcc cacgctccct ccggcgtacc gaagatcgcg tcgatccgcc cgttcaccac
    18241 ggctccgtcg atcgagagga ggaagggacg ctccgcgaac aacgggacgg cgtcggcgaa
    18301 ccggctcgtg aggaaggcct cgcggagctg ttgcaccttg cccggctctc ccgccagctc
    18361 ctcggccacc aggtcgggtg gttcctccag ctcgagcagg gaggcctggc ccgaggatcg
    18421 gcgctcgatc caagcgtgga cgtcggtgcc gatccgggcc gccgggccgg agaagcgagg
    18481 caggggacgg atcgtggtcc agtagaagcg cttcgggcac cgaccgtaat cgatgagccc
    18541 actgaccgaa acggccgtgg gcgccggtgg tgggacggcg gtcatggact cacgctctcg
    18601 caggtgcacg gcgagggttc gccgttcacc cgccagggtc tcataccggg cgcgttcctc
    18661 gggagacagc tcgctgagcg cggcctgggg gatcgccccg gttcgaacgg catggagggc
    18721 ggctcggcgc cagccatcgg ggaacagcgg atcgctctcg tccggccgag ccggtccggg
    18781 ccagctccgg acgaaccgct gccggtaccc ggccagcggg ttcgctccat cctcctcgcc
    18841 gccctggaag gagacgcgga cgccgggctc cccgcgggcc cacttggcca gctcgccgaa
    18901 gaagagccca acgcccttgg cccgcaacga ctctccgtac cagagcgcag acgagcagaa
    18961 cagccggcgc ttggccctgg tgagggcgac gtaggcggtg cgacgctcct cgaactcctc
    19021 ctggtctcgg agggcagcga cgaagtggct catcaccccg tcgaacgccg ggagcaggtc
    19081 gcggtcccgg cgcagctcga cgtcgagcga gcttccccgg cgcatggggt tctgctgcac
    19141 ccgggtgttg gggaacaggc ccccggccag tccagggacg aacaccgtat cgaactccag
    19201 gcccttggcc gcgtgcaccg tcatgacctt gacgctgtcg tcgtcggagg gctggaccgg
    19261 cgtccactca cgatcgtcct cgatcgagtc gacgtagtcg aggaacgctc gcagggtgag
    19321 ctccccctcc agtggctgga aggcgtgcac ctgatcgagg aaggccgata ggttccgggt
    19381 ccgcgccccc gccagcgccc ggtcgggatg cgcgtcgagc tcggccagca ggcccgagcg
    19441 ccggatcact tcggccagga actcgcccac agggcggcgc gccgctccgc gcaggtcggc
    19501 gagctcgccc gcaaaggcct cgagccgctc ccgaccctcc ggcgacaagc cctccacctc
    19561 gtccaggtgc tccagggcct ccgcgatcag gaacggctga tcctcgagca ggtcttcgtc
    19621 ctccggcatc cgatcggcca gccggtcgat gaaggaccac ccgctggaac gggtccatgc
    19681 ggcgacccgg gccaggtcgg ccaggcccac gcggtaccga ggcccggtca agatccgcgc
    19741 cagcgccacg ctggccgccg ggtctgccgc cgcccgagcg taggccagga tctcgaccac
    19801 ctccggcagc cggatcaggc cggccagccc gatgaactcg gccggcaccc cgcgatcctg
    19861 gaaggccagc tgcagcggct cgaagagccg gtgggtccgg cacaggatcg cgacgtcctt
    19921 ccaggccaca ccggtgccgt gcagctcctg gatccggtcc acgatcgcct cagcctcggc
    19981 cacctcatgc tcgtactggt cgacccacac gtcgccctcg ccgttcgggg ggtatggctc
    20041 cagctccttg ccggggtcgg ggcgctgctc gagggggagc ggacggatga cggtgccggc
    20101 ggcctccagg atccggccgc ccgatcggaa gttcatgtac aggggcagtc tgggagacgg
    20161 gctgcggtcg gccgagggga actgggtggg gaaccgcagc aggttgtgca ggctggcgcc
    20221 cctccaccca tagatgttct ggtcgggatc gcccaccgcg gtcaccggat gccccctgcc
    20281 gaacacggcc tccatcaaca gggcctgcgc gtggttcgtg tcctggtact cgtccaggag
    20341 cacggcctgg aagcggtcgc ggtaggcctg gaccacctcc gggtggtcct ccacgatccg
    20401 gaccgcatgg ccgatctgat cgccgaagtc gatcacgccg agccgccgct tcagttcgcg
    20461 gaaggccgag acgccgtcgg ccaactgcag tcgttcgagg gcggcacggt aggggtcggc
    20521 ggatcgggcg gcctgcagcc gctccaggtt cgcccgcacg aagcgccgga cctcttccgg
    20581 ctccacgcgg tggttctgga gctgttcgtc cagggacagg atgctcctca cgatcgaggg
    20641 ctgccacctg gtctgcaggt cggggaaggt catgaggtcc aacacgcgcc cggccagctc
    20701 ggcccgctgg gccggggtga tcaccctctg acccggctcg atcccgatca gcatcccgtg
    20761 gcggtccagg atctgggccg cgaagccgtg gtagttgagg atctcgggct cctcgccctc
    20821 gtccagccgc agcatgcgca gggccgcgcg gatccgcagt aggaggtgct cggtggcctt
    20881 gttcgtgaag gtcaggcaca ggacgttgcc gggcatcacc ccggggtgat cggcctggat
    20941 gtccccgttc gccaccatgg cgaggtagat cacccgagcc gccatcaccg acgtcttccc
    21001 ggagcccgca cccgccacca ggacgtacgg gacgaggggc atggagatcg ccgtccactg
    21061 ctcgggcgtg ggctcggcgc cgaggaagtc gaggatcggc ccagggtagc cagccatcat
    21121 gggctcgcgc ccccagtca
//
LOCUS       DASQQQ010000022        15661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_29610, whole genome shotgun sequence.
ACCESSION   DASQQQ010000022 DASQQQ010000000
VERSION     DASQQQ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15661
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29610"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..559
                     /locus_tag="VE646_01235"
     CDS             65..559
                     /locus_tag="VE646_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZD78650.1"
                     /translation="MSLLVRHAMTEAPKAASPDMTAADAAGLMRSFDVGVVPVVDGDR
                     LIGLVTDRDLVVRVLADRLDPTQVKLQEIATTSTVTISPDQQLSDARTLMAERQIRRL
                     PVTKDGRLVGILSMGDLAVADASKRAMGEALEDVSASEATKRVNEGPESGTPERVQER
                     REVT"
     gene            561..1148
                     /locus_tag="VE646_01240"
     CDS             561..1148
                     /locus_tag="VE646_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020683203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HZD78651.1"
                     /translation="MGLEEYRRKRNLRSTPEPAGGRRRPGHRPRFVIQKHDASSLHYD
                     VRLEADGVLKSWAVPKGPSTDPRDKRLAVPTEDHPLDYADFEGVIPQGQYGAGTVIVW
                     DTGTYENLTHDRRDEPTPVARAIEDGHLVVRLEGSKLTGGYAFTRTGSGDDARWLLVK
                     TDDEGADARRRPTSTQPESVISGRTVGEVAEASAR"
     gene            1145..2149
                     /gene="ligD"
                     /locus_tag="VE646_01245"
     CDS             1145..2149
                     /gene="ligD"
                     /locus_tag="VE646_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019359200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HZD78652.1"
                     /translation="MTSSIVHTLPDELRGRVRRRERPRWVKPMLATLTDERFSDPDWI
                     FERKLDGERCLAVRSGEAVKLFSRHRKSLNHSYPEIAEALAAQDPDTFLVDGEIVAFE
                     EGKTSFARLQGRMQLRDPDQARATGIAVFYYVFDLLRVEGHDLERLPLRARKALLRRA
                     LAFRDPIRLCTHRNADGERYYREACGKGWEGLIAKDATAPYVHSRSRTWLKFKCVNEQ
                     ELVIGGYTDPKGSRTGFGAILGGFYRDGDLRYAGKVGTGFDHQTLRNLRERFEQLSRR
                     TPPFSEDPREKDAHWVSPELVATVGFTEWTEDDKLRHPRFRGLRRDKDPRDVWKEVPS
                     "
     gene            2146..3054
                     /gene="ligD"
                     /locus_tag="VE646_01250"
     CDS             2146..3054
                     /gene="ligD"
                     /locus_tag="VE646_01250"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020039395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HZD78653.1"
                     /translation="MSPVLEIGGHRIDVGHPDKVFFPDAGITKGELVEYYERIANTIL
                     PFLRDRPLAMRRYPDGIDGHDFFQKDAPDHFPGWIRRETVGKQGGTVDHVVCDDAATL
                     VYLADQACIESHVFLSKVGALDRPDRMIFDLDPSQEDLSTLRDAARRFRDLLEEIGLV
                     PFVMTSGSRGFHVVVPLRAEDPFDEVRDLAQGIARLLATEDPDHLTVQTRKDQRGGRI
                     FLDYLRNAYAATSIPPYAVRARPSAPVATPLDWEELGDTEPRTYTIRNLFRRLGQKED
                     PWRGMQRRARTLDGARAELRRRVHSG"
     gene            3064..3339
                     /locus_tag="VE646_01255"
     CDS             3064..3339
                     /locus_tag="VE646_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78654.1"
                     /translation="MREGSGTAAGVGLGVFVFLLLLPFLLVVILYTFFTIYAMTKGLG
                     FDAHAIDLGVLFSGVAVLTAALLLLLMGVVALAGRSMDPRKHRRRGR"
     gene            complement(3361..3828)
                     /locus_tag="VE646_01260"
     CDS             complement(3361..3828)
                     /locus_tag="VE646_01260"
                     /inference="COORDINATES: protein motif:HMM:NF020989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HZD78655.1"
                     /translation="MPPARVKRTRKGEYLLRLTANERDVIRTLPETLREALAGDPQDP
                     ALRRLFPAAYPDDPARSQEFDELVRDDLLSERLSAVETMERTLDAQRVGEDELLAWLS
                     AINDLRLVLGTRIGVTEEMTFSDLAPDDPRVPSFALYSFLGVLEEDVVGALSR"
     gene            complement(3832..4125)
                     /gene="clpS"
                     /locus_tag="VE646_01265"
     CDS             complement(3832..4125)
                     /gene="clpS"
                     /locus_tag="VE646_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006131673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HZD78656.1"
                     /translation="MASTAPVEVDRPSGEEDVERDVPWVVVVWNDPVNLMSYVVYVFQ
                     KLFGYSRGKATKLMLDVHQKGKAAVSHGTRERCEFDVSRLHAHGLWATMQKDR"
     gene            complement(4144..7881)
                     /locus_tag="VE646_01270"
     CDS             complement(4144..7881)
                     /locus_tag="VE646_01270"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZD78657.1"
                     /translation="MIAFDRRYAAVPEAVAQARRALDDLTRALRPSCVDTLRLLVSEV
                     VTNAVRYGPRGRDSMVDLRVEFLDQTVRVEVEDRGHGFELRQGPAEDGASGWGLYLVD
                     QLAHRWGIGSGGHTHVWFELDGCVVPEEERPQGSWPATWDPLLLDELRAAVVGTDLDG
                     VITRWNGHAEQLFGWSAAEAIGGKVTELLVAPADAAAAEAIVDRLRRGEPWEGEWWAP
                     HADGSRVWIHVANAPVRHPDGSLLGMVGVSIETGERHRAQEELDRRLRELGESERRFR
                     LLSEGSPTAILAFDLSGEPLYANRRAEELTGFAGKELSPDRLLDQIHPADLARVRPLW
                     RRVLSGGEMRDIEFRVVEDDQSRWRRATWVPLLDERGRRIGIQGRIEDVTERREREAA
                     LAAREEQLRLALSAAGMGTWDWDIASGRVEWSEGLERIHGLPPGGFGGTFDDFQRDIH
                     REDREALLASIADAVSTGADYRHDYRIRLPDGTIRWLSVRGQVFHEGSTPVRMAGVGM
                     DITERKELEQRLELKDAVADVLTDAGSVESAAPAILEAVGTSMGWQLGTMWVVDEEQG
                     VLRPAGRWAAGSPAAPPHAPDTDLGPGVGLAGRVWSAASPVWIRDLAAEPELLVDPVG
                     DEMPVRSGFGFAAMLGRRVVGVLEFWSRSIRDPEPSSLQMVRVLGSQIGQFLERKGAE
                     QAVLESEAVKTAVLSSSLEAIITMDEVGRVVELNPAAETMFGIRREEVVGRELAAVLI
                     PEPYRARHREGLDAYRRTGRGAILGRRVEMTGLRSDGEEFPIELSVVRVDLPGPALFT
                     ATIRDIADRRRAQELRLDLLAKERAARGEAEAARDRLTFLADASVILASSLDTRRTLA
                     KVARLAVPRLADWCSVDLLEPDGSIHSVAVAHVDPEKIALAREYRLRFPPKPDRPRGA
                     FQAVRTGEPYLIHEISDEELRRNLADPEELSLGRQLGIRSIMVVPLVARGRGLGAVTF
                     ASAESGRLFGPPDLELAEDLARRAALAIDNAGLYEERSHIARTLQRSLLPSSLPEIPG
                     VEVAALYQPATITRTEVGGDFYDLFEIGPDAWGVAMGDVCGKGVEAAALTGLARHTVR
                     AAALRRSGPRVVLDDLNEALRREGGDRFCTVVYSRLERGDGGIRMITSCGGHPLPVLI
                     RAEGTVEPIAEPGTLIGIFEDLQLSQRVVELHPGDAVLFFTDGLIDPRVAVPMDEPAV
                     HELLGACAGLSAQQIVDRFKDAMEQAEALDDLALLALRVTS"
     gene            complement(7868..8263)
                     /locus_tag="VE646_01275"
     CDS             complement(7868..8263)
                     /locus_tag="VE646_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZD78658.1"
                     /translation="MPRASSREEGAVPGSDPVRAAVRRTDDVAILELGGDLDVSSVAV
                     VERSLSELEAEAPPRLVIDLRGLTFLDSSGLRMIVAADARARRDGRDLVLIRGPESVA
                     RVFRTTLLEWRLRMVDDPSEVPGWGDDRV"
     gene            complement(8388..8573)
                     /locus_tag="VE646_01280"
     CDS             complement(8388..8573)
                     /locus_tag="VE646_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010843246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HZD78659.1"
                     /translation="MGERSEEAKGRAKEAAGDLTGDKDLQREGELDQASAKAKRKADQ
                     AIDKAKEKLGDAIDDDS"
     gene            complement(8669..9061)
                     /locus_tag="VE646_01285"
     CDS             complement(8669..9061)
                     /locus_tag="VE646_01285"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZD78660.1"
                     /translation="MISGEAFVLEFPAEAQHVGTARSFASALVRQLGGADEMVEDVKL
                     AVSEACTDTVVSLGGRGAMQVRGYPGSVGLRLEIDAPGRFEELQGDGPGSAVSSAARL
                     ELIRSLFPDADLAEGADGPILRFTVPLG"
     gene            complement(9058..9393)
                     /locus_tag="VE646_01290"
     CDS             complement(9058..9393)
                     /locus_tag="VE646_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019901267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZD78661.1"
                     /translation="MELRLDTRQDGTWAILEVAGEVDLFTAPSLRERIVQLIEEGNRQ
                     ILVDLTQVGFMDSSGLGVLVAGIKRAREAGGDMSLVCREGPTLKVLAITGLDRVFPIY
                     ASVDDAVGS"
     gene            9457..9852
                     /locus_tag="VE646_01295"
     CDS             9457..9852
                     /locus_tag="VE646_01295"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZD78662.1"
                     /translation="MRSTSSGPIILSVPARAENVQLLRSVAGAAAARHDLTFDAIDDL
                     RIAVDEACAQLLRAGGTRLTLQVRAAEHGIEALVSIDAHDPAWPPEGIEHSLAWQVLK
                     GLSDEVGAELGAGGPVIRIAKRGVEATQP"
     gene            9849..10607
                     /locus_tag="VE646_01300"
     CDS             9849..10607
                     /locus_tag="VE646_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HZD78663.1"
                     /translation="MSTAKHPARDEHVEDDELFAKLPGDPAARDELVVRNLPLASYLA
                     RKFVGRGEALEDLEQVANLALLKAIDRFDATREVRFSTFATVTIVGELKRHLRDRGWS
                     VRVPRQLQEMAVRITRELPELWQELGRSPTIPEIGDRIDATEDDVLEAMDAAQAYAAE
                     SLDQPTGDGNLTPGEALGSIDDRLERMEGWATVAPLIEQLPERERRILYLRFFEDKTQ
                     SEIAAELGISQMHVSRLLSQVLSFLRDNVDESPR"
     gene            complement(11016..12233)
                     /locus_tag="VE646_01305"
     CDS             complement(11016..12233)
                     /locus_tag="VE646_01305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZD78664.1"
                     /translation="MASRLDQLARATPGTRDRYVDFLRAASIVAVVFGHWFIGMIHWD
                     HGVVRTTSAVGVTSYLWLGTWVFQVMPIFFFVGGFSNLVAFEAFQQRGNSAWMFIRSR
                     EVRLLGPSIVFLAVWTAVQLALHVADIGAPAGPRLWGDTRLLRGMLPPGATVPFGPLW
                     FLGVYGLVVALAPITVGLHRRYGLLVPVGLMAAAVLVDVVGFGLGHPGVRWFNVAFVL
                     LLPHQLGHAYGDGSMLRWPRWVLWGMVAAGLGGLWLLTNPWVFRFAGDVRFEWFPMIG
                     SYPKSLLGTDIEPISNAYPPTVCFLLGGIWSIGAVMLLRSKMSRWLQRSGPWKATIFV
                     NGIIMTLFLWHMTAYLIAVVLLHPFGFGTQLDSTPRWWMERLVWELVPAAILAMLIAL
                     FGRFERFGSNAHP"
     gene            12329..13231
                     /locus_tag="VE646_01310"
     CDS             12329..13231
                     /locus_tag="VE646_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZD78665.1"
                     /translation="MQEHVPHHREPFRSPLRDDANRDLVALVTGGGTGIGRATARELA
                     LTGAAVVICGRREAPLRSLADDLAAMGREILAVPADVREPKQAARVVDGALARFGRVD
                     VLVNNAGGQFEAPAERIGLKGWRAVHRLVVDAAWELTRSVAERSMIPNRRGLVVFIGF
                     SPRRGMPGMAHAAAARAALENLAGGLAIEWSRYGIRAVCVACGTIDTEALRAYGEGET
                     ERWAREVPLGRLGSPEEVASVIAFLSSPGGRYVTGTTIAVDGGLDAWGLATLPPPVER
                     VGQRAAGHDPPDLRADGLGPPERG"
     gene            complement(13474..14241)
                     /locus_tag="VE646_01315"
     CDS             complement(13474..14241)
                     /locus_tag="VE646_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZD78666.1"
                     /translation="MAELVLVRRDGPVALLILNRPHKLNALSSAVEENLDAALEAEEV
                     RDSRVVILSGQGGAFCAGADMTEMGEATPDKIIGYYRRLGDVYERVARLPQPSIAAIH
                     GYCLGGGFELVLACDFRIADRSAVFGLPEVPIGILPSSGGTHRLVRAVGVARAKELIL
                     LRRRVDASEALRLGVLTELVDQGAATGRAMDLARELAELPPLAVEVAKRATEAMAESS
                     RDAGLLVERFAYGLLSQTEEHARATRSFGEGAAPDPS"
     gene            complement(14234..15328)
                     /locus_tag="VE646_01320"
     CDS             complement(14234..15328)
                     /locus_tag="VE646_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HZD78667.1"
                     /translation="MRAAVLYEPGGPLVVEEVELDAPKGGEVRVRMVASGVCRSDWHE
                     VRGVHAMPMPIVLGHEGSGVVEELGEGVDGLAVGDHVVLSWLPYCGSCRRCAAGRPNL
                     CERMGAFSEGYLADGTTRLHRGEDRIRHNVPSSFAEQAVVPAATAIPVDPTLPLDRLA
                     LLGCAATTGVGAVLNTARVRPGESVVVIGCGGVGLSAVQGARIAGASPIVAVDVIPGK
                     VDLARQLGASEGVVAPPDEDPEAAVRAVVPDGADAVFEALGRPSTIELATRLTSPGGR
                     AVLVGMAPPDARVGVDALTMVVEERTVMGSWYGSCVPPRDLPLLAGLVVSGALRLDPM
                     IGRNCSLEQVNDALAAFERGEEARAVIVYG"
     gene            15463..>15661
                     /locus_tag="VE646_01325"
     CDS             15463..>15661
                     /locus_tag="VE646_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78668.1"
                     /translation="MRPRHLRDEDLRRHLEAWVARELISTEEAEAIRAFEVGTAPSPR
                     RVPLVTEVLAYLGAGLAIAAGV"
BASE COUNT         2424 a         5842 c         5255 g         2140 t
ORIGIN      
        1 gttttccgcc ccagccgctc gggtacaccc ggagaggacc gtccccaagc tgaggaggtg
       61 cgacatgtca ctcttggtgc gacacgccat gaccgaggct ccgaaggccg cgagccccga
      121 catgaccgcc gccgatgccg cgggtctgat gcggagcttc gacgtcggcg tggtgcccgt
      181 ggtcgacggc gaccgcctga tcggactcgt gacggaccgc gacctggtgg tccgggtgct
      241 ggcggatcgc ctggacccca cccaggtcaa gctccaggag atcgccacca ccagcaccgt
      301 gacgatctcg cccgaccagc agctgtccga cgcacgcacg ttgatggccg agcgtcaaat
      361 ccgccggctc cccgtcacca aggacggacg gctggtgggg atcctgtcca tgggtgacct
      421 ggccgtggcg gacgcctcca agcgcgcgat gggcgaggcg ctggaggacg tgtccgcatc
      481 cgaggcaacc aagagggtga acgaggggcc cgagtccggt acccccgagc gcgtccagga
      541 gcgccgcgag gtcacctgag atggggctcg aggagtaccg gcgcaaacgg aacctccgct
      601 cgacgccgga gccggcagga ggtcgccgac ggcccggtca ccgcccacgg ttcgtgatcc
      661 agaagcacga tgccagctcg ctgcactacg acgttcggct ggaggcggac ggggtcctca
      721 agtcctgggc ggtacccaag ggcccctcga ccgaccccag ggacaaacgc ctggccgtcc
      781 ccaccgagga ccatccgctc gactacgcgg acttcgaagg cgtgatcccg caggggcagt
      841 acggagctgg aaccgtcatc gtctgggaca cgggaaccta cgagaacctc acccacgacc
      901 gccgggacga gccgacaccc gtcgcccggg ccatcgagga cgggcatctc gtggtccggc
      961 tggagggatc caagctcacc ggcggctacg ccttcacccg gaccggctcc ggcgatgacg
     1021 cccgctggct gctcgtgaag acggacgacg agggagcgga cgcccggcga cgaccgacca
     1081 gcacccagcc ggagtccgtg atctccggcc ggacggtggg ggaggtcgcc gaggcatccg
     1141 cccgatgacc tcgtcgatcg tccacacgct tcccgacgag ctccggggcc gggttcgccg
     1201 acgcgagcgg ccgcgatggg ttaagccgat gctggccacg ctcacggacg agcgcttctc
     1261 cgaccccgac tggatcttcg agcggaagct cgacggcgaa cgatgcctcg ccgtccggag
     1321 cggcgaggcg gtgaagctct tctcgcgcca ccggaagtcc ctgaaccaca gctaccccga
     1381 gatcgccgag gcgctcgcgg cgcaggaccc ggacacgttc ctcgtggacg gggagatcgt
     1441 ggccttcgag gagggcaaga cgagcttcgc gcgcctccag ggacgcatgc agctccgcga
     1501 tccggaccag gcgcgcgcaa ccgggatcgc ggtcttctac tacgtcttcg acctgctgcg
     1561 ggtggagggc cacgacctcg agcggctccc gctcagggcg cgcaaggccc tgctgcgccg
     1621 ggcgctcgcc ttccgcgatc cgatccgcct gtgcacccac cgcaacgcgg acggtgagcg
     1681 ctactaccgg gaggcctgcg gcaaggggtg ggagggactg atcgccaagg acgccacggc
     1741 cccctacgtc cacagccggt cgcggacatg gctgaagttc aagtgcgtga acgagcagga
     1801 gctggtgatc ggcggataca cggatcccaa gggaagccgg accggcttcg gcgcgatcct
     1861 gggcggcttc taccgggacg gcgatctccg gtacgccggg aaggttggaa cgggcttcga
     1921 ccaccagacg ctgcggaacc tccgggagcg gttcgagcag ctctccaggc ggacgccgcc
     1981 gttctcggaa gatccccgcg agaaggacgc ccactgggtc tcgccggagc tggtggccac
     2041 ggtcggcttc accgagtgga cggaggacga caagctgcgg catccgaggt tccgagggct
     2101 ccgccgggac aaggatcctc gcgacgtgtg gaaggaggtg ccgtcatgag cccggtgttg
     2161 gagatcggcg gtcatcggat cgatgtcggg cacccagaca aggtgttctt cccggatgct
     2221 ggcatcacga agggcgagct ggtcgagtac tacgagagga tcgcgaacac gatcctcccc
     2281 tttctgcgcg atcgcccgct ggccatgcgg cggtacccgg acgggatcga cgggcacgac
     2341 ttcttccaga aggatgcccc ggaccacttc cccggatgga tccggcgcga gaccgtgggc
     2401 aagcaaggag gcaccgttga ccatgtcgtc tgtgacgacg cggcgaccct cgtgtacctg
     2461 gccgatcagg cgtgcatcga gtcccacgtc ttcctctcca aggtgggcgc gctcgatcgg
     2521 cccgatcgga tgatcttcga tctggatccc tcccaggagg acctatcgac gctccgtgac
     2581 gccgctcgca ggttccggga cctgctcgag gagatcgggc tcgtgccctt cgtcatgacc
     2641 tcgggctccc gtgggttcca cgtggtggtg ccgctccgtg ccgaggatcc cttcgacgag
     2701 gtccgcgacc tggctcaagg gatcgcccgc ctgctcgcga ccgaggaccc ggaccacctc
     2761 accgtccaga cgcggaagga ccagcgcgga ggacggatct tcctggacta cctccgcaac
     2821 gcttacgccg ccacctcgat ccctccctac gccgtccgtg ccagaccctc ggcgccggtg
     2881 gccacgcccc tggactggga ggagcttggg gataccgagc cccggaccta cacgatccgg
     2941 aacctgttcc ggaggctcgg ccagaaggag gatccgtggc gcgggatgca gcgacgtgcg
     3001 cggaccctgg acggtgctcg ggccgagctg cgccggcggg tgcacagcgg gtaggctggc
     3061 gctatgcggg agggctcggg gacggccgcg ggcgtcgggc tcggcgtctt cgtgttcctg
     3121 ctgctcctgc ccttcctcct ggtggtgatc ctgtacacgt tcttcacgat ctacgcgatg
     3181 accaagggcc tgggcttcga cgcccatgcc atcgacctgg gcgtgctgtt cagcggggtc
     3241 gcggtgctga cggccgccct gctcctgctc ctgatgggcg tggtggcgct ggccggccgc
     3301 tccatggacc cacgcaagca ccgccgacgc ggccgctgag cgaccgctcg accacagccc
     3361 tcagcgcgag agcgcgccga ccacgtcctc ctcgagcacc ccgaggaacg agtacagggc
     3421 gaaggaaggc acccggggat cgtcgggcgc cagatcggag aacgtcatct cttcggtgac
     3481 cccgatccgg gtccccagga cgagccggag gtcgttgatc gccgagagcc aggcgagcag
     3541 ctcgtcctcc ccgacccgct gggcgtcgag ggtccgttcc atcgtctcga cggcggacag
     3601 gcgctcggac agcaggtcgt cacggaccag ctcgtcgaac tcctgactcc gagcgggatc
     3661 gtcggggtag gccgccggga acagccgccg gagcgccggg tcctggggat cgcccgccag
     3721 agcctcgcgg agcgtctcgg ggagcgtgcg gatcacgtcc cgctcgttgg cggtcagccg
     3781 gagcaggtac tcgcccttcc gggtccgctt gacccgcgcc ggcggcatcc ctcagcggtc
     3841 cttctgcatg gtggcccaca ggccgtgagc gtgcaggcgc gacacgtcga actcgcaccg
     3901 ctctcgggtc ccgtgggaga cggcggcctt gcccttctgg tggacgtcca gcatcagctt
     3961 cgtggctttc ccgcggctgt acccgaagag cttctggaag acgtagacca cgtacgacat
     4021 caggttcacc gggtcgttcc acaccacgac cacccagggg acgtcgcgct cgacgtcctc
     4081 ctcgccgctg gggcggtcga cctcgaccgg ggcggtggaa gccatgccac caccatacgg
     4141 cgctcaggac gtgacccgca gagccagcag ggccaggtcg tcgagcgcct cggcctgttc
     4201 catcgcgtcc ttgaagcggt cgacgatctg ctgggcggac agcccggcgc aggccccgag
     4261 cagctcatgg acggcgggct catccatcgg tacggccacc cgcggatcga tcagcccgtc
     4321 cgtgaagaac aggacggcgt ccccgggatg gagctcgacc acccgctggg agagctgcag
     4381 atcctcgaag atgccgatca gcgtgcccgg ttccgcgatc ggctccaccg tcccttcggc
     4441 ccggatcagc acggggagcg gatgcccgcc gcacgaggtg atcatgcgga tcccgccgtc
     4501 gccgcgctcc agccgcgaat acacgacggt gcagaaccgg tcgcctccct ccctgcgcag
     4561 ggcctcattc aggtcgtcga gcaccacccg aggaccggac cgacgcagag cggcagctcg
     4621 caccgtgtgc cgggccagcc ccgtcaacgc ggccgcctcc acccccttgc cgcagacgtc
     4681 gcccatggcc acgccccacg cgtcggggcc gatctcgaac aggtcgtaga agtcaccccc
     4741 cacctcggtc cgggtgatcg tggccggctg gtacagggcg gccacctcca cccccgggat
     4801 ctcgggcagg ctgctcggca gcaggctccg ctgcagggtc cgggcgatgt ggctccgctc
     4861 ctcgtacagc ccggcgttgt cgatggccag ggcggctcgg cgggccaggt cttccgccag
     4921 ctccaggtcc ggggggccga acaggcgacc ggactcggcc gaggcgaagg tcaccgcccc
     4981 cagcccccgt ccccgagcca ccagcggcac caccatgatc gagcggatcc cgagctgcct
     5041 gcccagggac agctcctccg gatcggcgag gttcctccgc agctcctcgt cgctgatctc
     5101 gtggatcagg tacggctcgc cggtccgcac cgcctggaag gcgccgcgtg ggcggtcggg
     5161 cttcggcggg aaccggagcc ggtactcgcg cgccagggcg attttctccg ggtccacgtg
     5221 ggccaccgcc accgagtgga tcgagccgtc cggctccagg aggtccacac tgcaccagtc
     5281 ggccagccgc ggcaccgcca gccgagccac cttggccagc gtccttcggg tatcgagcga
     5341 ggaggccagg atcacgctgg cgtccgccag gaaggtcagg cgatcacgag ccgcctcggc
     5401 ctcgccccgc gcggctcgct ccttggccaa cagatccaaa cgcagctcct gcgcccggcg
     5461 tcgatccgcg atgtcgcgga tcgtggccgt gaacagcgcc gggcccggca gatccacccg
     5521 caccacggac agctcgatcg ggaactcctc tccgtccgat cgaagaccgg tcatctccac
     5581 gcgccgaccc aggatggcgc cgcgaccggt ccgccggtac gcatccagcc cctcccgatg
     5641 ccgggcccgg tacggctcgg ggatcagcac cgccgccagc tctcgtccca cgacctcctc
     5701 gcggcggatg ccgaacatcg tctcggccgc ggggttcagc tccacgaccc tgcccacctc
     5761 gtccatcgtg atgatggcct ccagggatga gctcaggacc gccgttttca ccgcctcgct
     5821 ctcgaggacc gcctgctcgg cgcccttccg ctccaggaac tgcccgatct ggctgcccag
     5881 gacccggacc atctgcagcg acgacggctc cggatcgcgg atgctccggc tccagaactc
     5941 gagcaccccc accacccgcc gaccgagcat cgccgcgaac ccgaacccgg accgtacagg
     6001 catctcgtcc ccgaccggat ccaccaggag ctccggctcg gccgccaggt cgcggatcca
     6061 caccggggag gcggcggacc agacccggcc cgccaggccg acgccgggtc cgagatccgt
     6121 gtccggcgcg tggggaggcg ccgccggact cccggccgcc caccgccccg cgggacgcag
     6181 cacgccctgc tcctcgtcga ccacccacat cgtgccgagc tgccagccca tggaggtccc
     6241 cacggcctcc aggatcgccg gcgccgcgct ctccaccgaa ccggcgtccg tcaggacatc
     6301 cgccacggca tccttcagct cgagccgctg ctcgagctcc ttgcgctcgg tgatgtccat
     6361 gccgacgccg gccatccgca ccggcgtgga cccctcgtgg aagacctgtc cccggaccga
     6421 gagccagcgg atcgtcccgt cggggagccg gatccggtag tcgtggcggt agtcggcccc
     6481 cgtgctcacg gcgtccgcga tggaggccag cagcgcctcc cggtcctccc gatggatgtc
     6541 ccgctggaag tcgtcgaagg tgccgccgaa gccccccggg ggcaacccgt ggatccgctc
     6601 caacccctcc gaccactcga cccggcccga cgcgatgtcc cagtcccagg tccccatccc
     6661 ggcggcggac agcgccaaac ggagctgctc ctcccttgcc gccagcgctg cttcccgctc
     6721 cctgcgttcc gtcacgtcct cgatccgtcc ctggatcccg atccgccgtc cccgctcgtc
     6781 gagcagtggc acccaggtgg ccctgcgcca ccgcgactgg tcgtcctcca cgacccggaa
     6841 ctcgatgtcg cgcatctcgc cgcccgagag gacccggcgc cagagcggcc ggacccgcgc
     6901 caggtccgcg ggatggatct ggtccaggag ccggtccggc gaaagctcct tgcccgcgaa
     6961 ccccgtgagc tcctcggctc gccggttcgc atacaggggc tcaccggaca gatcgaaggc
     7021 caggatcgcg gtcggggagc cctccgacag caggcggaac cggcgctcgg actcgccgag
     7081 ctcccgcagc cggcggtcga gctcctcctg cgcccggtgt cgctccccgg tttcgatcga
     7141 cacacccacc atccccagca gcgatccgtc gggatggcgg acgggcgcgt tcgccacgtg
     7201 gatccacacc cgggagccat cggcgtgggg agcccaccac tcgccctccc aaggctcacc
     7261 tcgacggagc cggtccacga tcgcctcggc ggccgccgcg tcggccgggg cgaccagcag
     7321 ctcggtgacc ttcccgccga tcgcctccgc cgccgaccag ccgaagagct gctccgcatg
     7381 gccgttccac cgggtgatca ctccgtccag gtcggtgccc accaccgcgg cccggagctc
     7441 gtcgagcagc agcggatccc aggtggcggg ccacgatccc tgggggcgct cctcctcggg
     7501 caccacacat ccatccagct cgaaccacac gtgggtgtga ccgcccgatc cgatccccca
     7561 tcggtgagcg agttggtcca cgaggtacag gccccaaccg gacgcgccgt cctcggccgg
     7621 gccctgccgg agctcgaacc cgtgcccccg atcctcgacc tcgacccgca cggtctgatc
     7681 caggaactcg acccgaaggt cgaccatgga atcgcgtccc cgggggccgt agcggacggc
     7741 gttcgtgacg acctcgctga ccaggagacg gagggtatcc acgcagctcg gacggagggc
     7801 ccgcgtcaga tcgtccagcg cgcgccgcgc ctgggctacg gcttcgggaa cggccgcgta
     7861 ccggcggtca aacgcgatca tccccccacc ccggaacctc cgacggatcg tcgaccatcc
     7921 gcagccgcca ctccaacagc gtagtgcgga agacacgagc cacgctctcg gggcctcgga
     7981 tcagcaccag gtcacgtccg tccctgcggg ccctggcatc ggccgccacg atcatccgca
     8041 acccgctgga atccaggaac gtcagcccgc gcaggtcgat caccagccgg ggcggcgcct
     8101 cggcttccag ttcggagagc gagcgctcga cgacggccac ggaggacaca tccaggtcgc
     8161 cgcccagttc caggatggcg acatcatcgg tcctgcggac ggccgcgcgg accggatccg
     8221 atccgggtac ggccccctcc tctcggctcg atgctcgcgg cacgggctcg ccgcgggctc
     8281 ccttccccgg ctgagggacc gttcaaacgt cctggggccg ctcggctgcg gccggacgcg
     8341 ccgggcgggg agcggaggct gccgctcccc gccctggtct cggcacgcta ggagtcgtcg
     8401 tcgatggcgt cgccgagttt ctccttggcc ttgtcgatcg cctgatcggc cttgcgcttg
     8461 gccttggcgc tggcctgatc gagctcgccc tcgcgctgca ggtccttgtc gccggtgagg
     8521 tcgccggctg cttccttggc ccggcccttc gcttcctccg aacgctcacc catgcccact
     8581 cccttcgccg tccttcggtc attcccggac ccttccggcg ggaaacgagg caccccccgc
     8641 agatggtggc gataacggga cggcgtggtc agccaagcgg gacggtgaac cgaaggatcg
     8701 ggccgtcggc cccttcggcc aggtcggcat ctgggaagag cgagcggatc agctcgagcc
     8761 tggcggccga ggacacggcc gaaccaggtc cgtcgccctg cagctcctcg aaccggccgg
     8821 gggcgtcgat ctccaggcgg aggccgaccg accccggata gccccggacc tgcatcgccc
     8881 cccgcccgcc gaggctcacg acggtatccg tgcaggcctc ggagacggca agcttgacgt
     8941 cctcgaccat ctcgtcggcg ccaccgagct ggcgcacgag cgcgctggcg aagctccggg
     9001 ccgtgccgac gtgctgggcc tctgccggga actccaggac gaacgcctcg ccggagatca
     9061 cgagccgacg gcgtcgtcca cggacgcgta gatcgggaag acgcgatcca acccggtgat
     9121 ggcgagcacc ttcagcgtcg gcccctcccg gcacaccagc gacatgtcgc cgccggcctc
     9181 ccgagcccgt ttgatcccgg ccaccagcac gcccaacccg ctggagtcca tgaacccgac
     9241 ctgggtgaga tcgaccagga tctggcggtt gccctcctcg atgagctgga cgatccgttc
     9301 gcgtagggac ggagcggtga acaggtcgac ctcgccggcg acctccagga tcgcccacgt
     9361 gccgtcctga cgggtgtcga gtcgaagttc gatgacgccc tcctcgcgtt ggagccgtga
     9421 gcgttcgacc acggcctggc tagtataggt ctgctcgtgc gttcaacctc aagcggtccc
     9481 atcatcctct ccgttcctgc acgagcggag aacgtccagc tcctgcgcag cgtcgcgggg
     9541 gcggctgcag cgcgccacga tctgaccttc gacgcgatcg acgatctccg gatcgccgtg
     9601 gacgaggcct gcgcccagct gctccgcgcc ggaggtacgc gcctcacgtt gcaggtgcgg
     9661 gccgcggagc atggcatcga ggcgctcgtc tcgatcgatg cacacgatcc cgcgtggccg
     9721 ccggaaggga tcgagcactc gctcgcctgg caggtgctga aggggctctc ggacgaggta
     9781 ggagccgagc tcggggccgg tggaccggtg atccggatcg ccaagcgagg ggtagaggcc
     9841 acccagccat gagcacggcc aagcaccccg cacgcgacga gcacgtcgag gacgacgagc
     9901 tgttcgccaa gctccccggc gacccggcgg cccgggatga gctggtggtg cgcaaccttc
     9961 ccctggcctc ctacctggct cgcaagttcg tgggccgtgg cgaggccctc gaggacctgg
    10021 agcaggtcgc caacctggcc ctgctgaagg cgatcgaccg cttcgacgcc acccgggagg
    10081 tgcggttctc gaccttcgcc acggtcacga tcgtggggga gctcaaacgg cacctgcgcg
    10141 accggggatg gtccgtccgc gtgcctcgcc agctccagga gatggccgtc cgcatcacgc
    10201 gcgagctccc cgagctgtgg caggagctcg gccgatcccc cacgatcccc gagatcgggg
    10261 accggatcga cgccaccgag gacgacgtgc tcgaggccat ggatgcggcg caggcatacg
    10321 cggccgagtc cctggaccag cccacgggcg acgggaacct cacgccggga gaggcactcg
    10381 gctcgatcga cgaccggctg gagcggatgg agggatgggc cacggtggct ccgctcatcg
    10441 agcagctgcc cgagcgggag cgccggatcc tctacctccg gttcttcgag gacaagaccc
    10501 agagcgagat cgcggcggag ctcgggatct cgcagatgca cgtctcgcga ctgctctcgc
    10561 aggtgctgtc cttcctgcgc gacaacgtcg acgagagccc ccgctgagac gcaccgaccg
    10621 gcacgctccc gctccgcccg ccgcgtttgg ggcgatgcgg ccaagggtat ccgtcggccg
    10681 agatggcgaa ccagatggat acgaacgaac cgatcttccg gggcagcagg gtggctcctc
    10741 ggggacggcg gcagcggacc gcgagaccgg gacgggtctg tgccagcccc gggtgcacca
    10801 cggtcctgtc cgtctacaac cacgaggaca gctgctggcg gcatgctccg gtgcacgcct
    10861 accacgttcg agggcgcagg tccggcggtc gcgcagcctg agcgatcgcc ctcgcgctcg
    10921 agtcggcaga cgtgctcgtc cggggggtcg gtccccctcg ggtagccccc ttccccccgc
    10981 cggggccggc ccctcttctc ctcgggcgaa gcgagtcacg ggtgggcgtt cgagccgaag
    11041 cgctcgaacc gcccgaacag cgcgatcagc atcgcgagga tcgcggccgg caccagctcc
    11101 cagaccagcc gctccatcca ccaccgcggt gtgctatcca gctgggtccc gaaaccgaac
    11161 gggtggagca ggacgaccgc gatcaggtac gcggtcatgt gccagaggaa tagcgtcatg
    11221 atgatgccgt tcacgaagat cgtggccttc cacgggccgg atcgctgcag ccagcggctc
    11281 atcttcgagc ggaggagcat cacggcgccg atggaccaga tcccccccaa caggaaacac
    11341 accgtcggag ggtaggcgtt ggagatcggc tcgatgtccg tccccaacag gctcttgggg
    11401 taggagccga tcatcgggaa ccactcgaag cgcacgtctc ccgcgaagcg gaagacccag
    11461 gggttggtca gcagccacag accgcccagg cccgcggcca ccatgcccca caagacccat
    11521 cggggccagc ggagcatgct cccgtccccg tacgcatgac cgagctgatg cggcagcagc
    11581 aggacgaagg ctacgttgaa ccagcgtacg ccggggtgcc ccaggccgaa cccgaccacg
    11641 tccaccagta ccgcggccgc catcagcccc accggcacca gcaggccgta ccggcgatgc
    11701 agcccaaccg tgatcggcgc cagcgccacc accaggccgt agacgcccag gaaccacagc
    11761 ggcccgaacg gcaccgtggc ccctgggggc agcatgccgc gcaacagcct cgtgtcgccc
    11821 cagagccggg ggcccgctgg ggctccgatg tccgccacgt ggagggccag ctgcaccgcc
    11881 gtccacacgg ccaggaacac gatcgaggga cccagcagtc ggacctcacg gctgcggatg
    11941 aacatccagg cgctgtttcc ccgctgctgg aacgcctcga aggcgaccag gttggagaag
    12001 ccccccacga agaagaagat cggcatcacc tggaagaccc aggtcccgag ccacaggtag
    12061 gaggtcaccc ccaccgcgct ggtggtccgg accacgccat ggtcccagtg gatcatgccg
    12121 atgaaccagt ggccgaacac caccgccacg atggaggcgg cccgcaggaa gtccacgtac
    12181 cggtcccggg tccccggtgt ggccctcgcc agctgatcca ggcggctcgc catgaccctc
    12241 ccgggcaacg gtataccggc cgcgccctcg atggaacgaa ccgctcgact cgcgaggcca
    12301 tcgccgctcg ggcatgatga gcccgccgat gcaggagcac gttccacacc accgtgagcc
    12361 cttccgctcg ccgctccgcg acgacgccaa ccgcgacctg gtggcgctcg tgacgggcgg
    12421 cgggaccggc atcggccggg ccacggcccg cgagctggcc ctgacgggtg ccgcggtcgt
    12481 gatctgcggc cggagagagg cacccttgcg gtcgctcgcg gacgatctgg cagcgatggg
    12541 acgggagatc ctggcggtac cggcggacgt ccgggagccc aaacaggccg ccagggtggt
    12601 ggacggcgcc ctggcgcgct tcggtcgggt ggacgtgctg gtcaacaacg ccggcggcca
    12661 gttcgaggcc cccgccgaac ggatcggcct gaagggctgg cgggcggtcc accgcctggt
    12721 ggtggacgcg gcctgggagc tcaccaggtc cgtggccgag cgttcgatga tccccaaccg
    12781 caggggcctg gtggtcttca tcggcttcag cccccgccga gggatgcccg ggatggccca
    12841 tgccgcggcg gcccgcgctg ccctggagaa cctggccggc ggcctggcga tcgaatggag
    12901 ccgctacggg atccgtgccg tctgcgtggc ctgcggcacc atcgacaccg aggccctccg
    12961 cgcgtacggg gagggggaga ccgaacgctg ggcccgagag gtccccctgg gtcgcttggg
    13021 atccccggaa gaggttgcct cggtcatcgc gttcctgtcc tcgcccgggg gacgctacgt
    13081 gacggggacc accatcgcgg tggatggcgg gttggacgcc tgggggctgg ccaccctgcc
    13141 gccgccggtg gagagggtcg ggcagagggc cgccgggcac gacccgcctg atctccgggc
    13201 ggatggtttg gggccgccgg agcgggggta gatgggcctc gagatcaccg aacgcgcggc
    13261 gaccagcggc cctaggcccc ggccatgtgg cgcccacgcc ggggcagcgc tgaggtggtc
    13321 cgcccccctt gggcccggat gttcgcatcc gggcccaacc cttgtcccgg ctccttcgct
    13381 gctggtcccg ctcgccccgc tcccgaccgg ctgcctgaac cagagccagc gggcacgctc
    13441 cgccccaccc gaggcccgga ccggcgccgc gtctcaggac gggtcgggag cggccccctc
    13501 cccgaatgac cgggtggccc gcgcgtgctc ttccgtctgg gagagcaggc cgtaggcgaa
    13561 gcgttccacc aggagcccgg catccctgga cgactcggcc atggcctcgg ttgctcgctt
    13621 ggccacctcc acggcgagcg gcggcagctc cgcgagctcc cgagccagat ccatcgcgcg
    13681 ccccgtcgcc gccccctggt ccacgagctc cgtgagcacc cccaaccgga gcgcttccga
    13741 ggcgtccacg cgccggcgca gcaggatcag ctccttggcc cgcgccacgc cgacggctcg
    13801 caccagccga tgggtccctc ccgaggacgg gaggatcccg atcggcacct cgggcagtcc
    13861 gaacacggca ctgcggtcgg cgatccggaa gtcgcaggcc aggaccagct cgaagccacc
    13921 ccccaggcaa tagccgtgga tcgccgcgat cgagggctgc ggcagccgcg ccacccgttc
    13981 gtagacgtct cccagcctcc ggtagtagcc gatgatcttg tccggcgtgg cctcgcccat
    14041 ctccgtcatg tcggcgccgg cgcagaacgc tcctccctgt ccggacagga tcaccacccg
    14101 gctgtcccgt acctcctcgg cctccagtgc ggcatccagg ttctcctcca cggcgctgga
    14161 gagcgcgttc agcttgtggg gacggttcag gatcagcagc gccaccgggc cgtcgcggcg
    14221 gaccaggacc agctcagcca tacacgatca ccgcccgcgc ttcctcgccc cgttcgaagg
    14281 cggccagcgc gtcgttgacc tgctcgaggg agcagttccg cccgatcatc ggatccaggc
    14341 ggagcgctcc ggaaacgacc aggcccgcca acaggggtag gtctctgggc gggacgcacg
    14401 agccgtacca gctgcccatg acggtccgct cctccaccac catggtcagg gcatcgaccc
    14461 cgacccgggc atccgggggc gccatgccca ccagcaccgc tcggccgccc ggtgaggtca
    14521 gcctggtggc gagctcgatc gtgctcgggc gccccagggc ctcgaagacg gcgtcggctc
    14581 catcgggaac cacggcccgg accgccgcct ccgggtcctc gtcgggcgga gccaccaccc
    14641 cctcggaggc ccccagctgc cgcgcgaggt ccaccttgcc ggggatcacg tccaccgcga
    14701 cgatgggcga ggcacccgcg atccgcgccc cctgcaccgc ggataggccc accccaccgc
    14761 acccgatcac caccaccgac tccccgggac ggaccctcgc cgtgttcagt acggctccca
    14821 cgccggtggt cgccgcgcac ccgagcagcg ccagccgatc cagcgggagc gtggggtcga
    14881 cggggatcgc cgtggccgcc gggaccaccg cctgctcagc gaaggacgag ggcacgttgt
    14941 gccggatccg gtcctcaccc cggtgcagcc gggtggtgcc gtcggccagg tacccctcgc
    15001 tgaaggcccc catccgctcg cacaggttgg gacgtccggc cgcacagcgg cgacaggatc
    15061 cgcagtacgg cagccaggac agcaccacgt ggtcccccac ggccagcccg tccacaccct
    15121 cgccaagctc ctcgaccacg ccggagccct cgtggcccag gacgatcggc atcggcatgg
    15181 cgtgcacgcc gcgcacctcg tgccagtccg agcgacagac cccgctcgcc accatgcgta
    15241 cccggacctc gccccccttc ggagcatcga gctcgacttc ctccaccacc agaggaccac
    15301 cgggctcgta gaggaccgcc gcgcgcacgg gggcaagtat cccccatgtc ggcagtgggg
    15361 gacgggtcgc aggatgtccc gtcggacgtc ggccgaacgg ccctcgatgc cccccccgtc
    15421 ccggtcgcac catcaccccc gggaggtgcc accatgtcca ccgtgcgtcc acgccacctc
    15481 cgcgatgagg atctgcgacg ccacctcgag gcctgggtgg cccgggagct gatcagcacc
    15541 gaggaggccg aggcgatccg cgccttcgag gtgggaacgg ccccctcgcc ccgtcgcgtc
    15601 cccctggtga cagaggtgct ggcctacctc ggcgcggggc tggcgatcgc cgcaggggtc
    15661 g
//
LOCUS       DASQQQ010000023         7165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_167622, whole genome shotgun sequence.
ACCESSION   DASQQQ010000023 DASQQQ010000000
VERSION     DASQQQ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7165
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167622"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VE646_01330"
     CDS             <1..548
                     /locus_tag="VE646_01330"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HZD78669.1"
                     /translation="IVGGFYARIGNLELLWVAAAACLGAIVGDNVGYLIGHRFGRGFL
                     ERHGHRMFITDERLERADRYYGSHGGKTVFLARFIPVVRSVGSILAGVAHMPWRRFLA
                     YDAAGAILWGVGHSILGYAIGASYERWKGYLTPAGLAILLVLLLLIAGSKLLAARRKV
                     EGQVLSGQEGSVGVKHDTSEE"
     gene            552..1640
                     /locus_tag="VE646_01335"
     CDS             552..1640
                     /locus_tag="VE646_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HZD78670.1"
                     /translation="MDVSELLRYTVDTGASDLHLKAGNVPFIRVDGDLRPGSFPALSP
                     TDTDAIAQALMPEHKAREFSATSEADFGYSLAGVGRFRVNVYRQRGTVGLAVRRVRSE
                     VPTFEELFLPPVIRQLAESSRGLVLLTGPTGTGKTTTIASMIGHINRTRRAHIVTVED
                     PIEVVHEDEMSIIEQREIGLDTESYAAALRHVIRQDPDVIFIGEIRDPESALSGIQAA
                     ETGHLVISTMHTIDATETINRLLDLFPPQQQREVRSSFAGALRGIVSQRLLPRTDGRG
                     RVPAVEVLVATGRVYDRIIDPEATVEIHDIIADGEYYGMQSFDQALVELVQQGLVSEE
                     DARRFSTNPHDFDLALRGVMTRGTPQLT"
     gene            1690..3093
                     /locus_tag="VE646_01340"
     CDS             1690..3093
                     /locus_tag="VE646_01340"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019525369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HZD78671.1"
                     /translation="MTEPTRPQPGARLDVHPLATELGERFRAAGFQLYLVGGVVRDLL
                     LGRVREGAELDMATDATPTQTTRVLRGWADRRYLAGARFGTVGARKGDTSFEITTFRQ
                     EVYHEEHRRPSVTFAKDLETDLVRRDFTINAMAIGLPGGGFVDPCGGVRALAARRLDT
                     PLDPEVAFSDDPLRMLRAARFLSQLEVTPTPRVVQAIRELRDRLDIVSAERISDELSK
                     LLVGSRPSKGLDLVVSTGLAERFLPELPALTVEQDPVHKHKDVLRHTYAVVERCEPDL
                     VLRLAALLHDIGKPKTRRIGAEGVSFHHHEVVGARMARERLVALRYPSAVVEDVVRLV
                     EMHLRFHGYSEWTDSAVRRYVREAGPLLDRLNQLTRADCTTRNPFRARQLAALQDDLE
                     ERIARLGEEENLQAIRAPLDGNQVMEHLGLAPGPEVGRALAYLLDERMERGPIEEDEA
                     YRLLDAWAKERGIARRS"
     gene            complement(3154..3918)
                     /gene="lepB"
                     /locus_tag="VE646_01345"
     CDS             complement(3154..3918)
                     /gene="lepB"
                     /locus_tag="VE646_01345"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZD78672.1"
                     /translation="MSISDELPAPDDAVRPDEGSSGDPEQPSGLTTMEGDDRQGVSGA
                     EPQRGRHRKEREDRGSFLREVPALVLIALVVAILIKTFLVQAFFIPSASMEPTLVPGD
                     RVLVNKLAYRFGDIHRGDVVVFSNPQVVSATDRNWFQGVIHWLGEGIGVAQPPDEDLI
                     KRVIGLPGDTVQIRGHAVFVDGQRIAEPYLTAQAKASMQDFGPERVPPGQLFVMGDNR
                     GNSADSRFTLGPVPERDVIGKAFVIIWPPSDLGGLG"
     gene            complement(3946..5025)
                     /locus_tag="VE646_01350"
     CDS             complement(3946..5025)
                     /locus_tag="VE646_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD78673.1"
                     /translation="MSLRVEVRARRGGFAVQARLDAEDGETVALLGPNGAGKSTLVEV
                     LSGLLPPEHGAVVLDGRVLDDVRARIHVPPEARPVGVVFQDRLLFPHLSAVENAAFPL
                     RARGVPRDEARRRASELLARLGLSGRDADRPASLSGGQAQRVALARAVIHGPRLLLLD
                     EPLSALDVSARAQLRALIREVVGSFPGARLLVTHDPVEATTLADRMVIVEEGRVTQSG
                     TPRQIRDRPRSSYAADLVGLNWFSGRLERMPDGAGRIRTSDGDVVVGWPPDLDPSVTT
                     VFGLLRPADVAIHARPPEGSARNVVHGRVGAVAADAERVRVHIASRPPVVAEITTGSL
                     DRLGLRPGDEVWASFKAVEVRVVPP"
     gene            complement(5022..5819)
                     /locus_tag="VE646_01355"
     CDS             complement(5022..5819)
                     /locus_tag="VE646_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD78674.1"
                     /translation="MSWTPRRGLTPLPIAVLAAAGAALVALPLAGLALRTPWSKAWAA
                     VASPVALTALRLSLVVATSASVIAFVVGLPLAWVLARSRVPGRTILRGIVVLPLVMPP
                     VVGGVALLTAFGRRGLVGAPLYRLLGVQITFTTGAAILAATFVSFPLMVLALEAGLRA
                     MDLRLEQAAVTLGASRWYVLRRVTLPLLAPQIVAGFVLSWARALGEFGATITFAGNLR
                     GVTQTLPLAVFQELQTDPGGAVALSLSLVAISLAVIVGLRARFLSAP"
     gene            complement(5816..6625)
                     /gene="modA"
                     /locus_tag="VE646_01360"
     CDS             complement(5816..6625)
                     /gene="modA"
                     /locus_tag="VE646_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018798465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZD78675.1"
                     /translation="MSSAEGGRRSGPVVALALIAAVGLFGCGPAEGRTGGSSSSGGRL
                     TVLAAASLTQVFTAIGRDFERGHPGSTVRFSFGPSDGLAEQIIEGAPGDVYASASPVE
                     MDRVQTQGPGVDHRGDFARNSLTVITPADDPAGIVSLRDLTRPGVELVLAAEGVPAGD
                     YAREVLRNAGILAAATANVVSNESDVKGVVQKVLLGEADAGIVYRTDVTPDVASRVRQ
                     IDIPPSLNVMADYPIAVVDGSPHPDLAAAFVRYVLGPGQEVLRRYGFGPPG"
     gene            complement(6622..7011)
                     /locus_tag="VE646_01365"
     CDS             complement(6622..7011)
                     /locus_tag="VE646_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZD78676.1"
                     /translation="MEASYRIGEAAELLGVRVETLRRWERQGKLQTRRTEGGQRRVPA
                     EEVARLLGERRERPRVTSASVRNRFPGIVTHVETDGVAATVELLAGPHRILALITREA
                     VEEIGLSPGMAAVATVKATNVMIEVDR"
BASE COUNT         1088 a         2574 c         2555 g          948 t
ORIGIN      
        1 tcatcgtcgg cggattctat gcgcggatcg ggaaccttga actgctctgg gtggctgccg
       61 cggcctgcct gggagcgatc gtgggcgaca atgtcgggta cctgatcggc catcggttcg
      121 gccgagggtt cctggagcgg cacggccaca ggatgttcat caccgacgag cgcctggagc
      181 gcgccgaccg ctactacggg tcccacggcg gcaagacggt gttcctcgcc cggttcatcc
      241 cggtggtgcg aagcgtggga tccatcctgg ccggggtggc gcacatgccg tggcggcggt
      301 tcctggcgta cgacgcggcc ggggccatcc tgtggggagt gggccactcc atcctggggt
      361 acgcgatcgg tgccagctac gaacgctgga agggctacct tacgccggcc gggttggcca
      421 tcctgctggt cctgctgctc ctgatcgcgg gctcaaaact gctcgccgcc cgccgaaagg
      481 tagaggggca ggtcctctcg gggcaggagg ggtcggtggg agtgaagcac gacacgagcg
      541 aggagtgatc ggtggacgtc tctgagctgt tgcgctacac ggtcgatacg ggcgcgagcg
      601 atctgcacct gaaggccggc aacgtgccgt tcatccgcgt ggacggcgac ctgcggcccg
      661 ggagcttccc cgcgctctcg cccaccgaca ccgatgccat cgcccaggcc ctgatgcccg
      721 agcacaaggc ccgcgagttc tccgccacca gcgaggcgga cttcggctat tccctggccg
      781 gggtggggcg gttccgggtc aacgtgtacc gccagcgcgg aacggtgggg ttggccgtcc
      841 ggcgggtccg ctccgaggtt cccacgttcg aggagctctt cctgccgccg gtgatccggc
      901 agctcgccga gtcctcgcgg ggcctggtgc tgctcaccgg gccgaccggg accggcaaga
      961 ccaccacgat cgcctcgatg atcgggcaca tcaaccgcac ccggcgggcg cacatcgtga
     1021 cggtggagga cccgatcgag gtggtgcacg aggacgagat gtccatcatc gaacagcggg
     1081 agatcggtct ggacaccgag tcctacgcgg ccgccctccg ccacgtgatc cgtcaggacc
     1141 ccgacgtgat cttcatcggg gagatccgcg accctgagtc ggcgctctcc gggatccagg
     1201 cggccgagac cggtcacctg gtcatctcga cgatgcacac gatcgacgcc accgagacca
     1261 tcaaccggct gctcgacctg ttccctccgc agcagcagcg ggaggtccgc tcctcgttcg
     1321 cgggagcgct ccggggcatc gtctcccagc gcctgctgcc caggaccgac ggcagggggc
     1381 gggtgcccgc cgtcgaggtc ctggtggcca ccggccgcgt ctacgaccgc atcatcgacc
     1441 cggaggcgac ggtggagatc cacgacatca tcgccgatgg tgagtactac gggatgcaga
     1501 gcttcgacca ggcgctggtg gaactggtgc agcaggggct ggtctccgag gaggacgctc
     1561 ggcgcttctc caccaacccg cacgacttcg atctggcgct gcgcggcgtc atgacgcggg
     1621 ggacgccgca gctcacctag ccttcggacc ggcggtggca ggcaaggagg cgggtatcct
     1681 tgcccggtca tgaccgaacc cacgcgtccc cagccgggcg cgcgcctcga tgtccatcct
     1741 ctcgccaccg agctcgggga acggttccgg gccgccggct tccagctcta cctggtgggc
     1801 ggtgtggtgc gcgacctgct cctgggccgg gtccgggagg gagcggagct cgacatggcc
     1861 accgacgcaa cccccacgca gaccacccgg gtcctgcggg gatgggcgga tcgtcggtac
     1921 ctggccggag cgcgcttcgg cacggtgggc gcccgcaagg gggacacgag cttcgagatc
     1981 accaccttcc gccaggaggt ctaccacgag gagcatcgca ggccgtcggt cacgttcgcc
     2041 aaggacctgg agaccgacct ggtgcgccgg gacttcacca tcaacgcgat ggccatcggc
     2101 ctgcccggcg gagggttcgt cgacccgtgc ggcggcgtgc gggcgctggc ggcccggcga
     2161 ctcgacacgc cgctggatcc cgaggtggcg ttctccgacg accccctgcg gatgctccgc
     2221 gcggcccggt tcctctccca gctggaggtg accccgaccc cccgggtcgt ccaggccatc
     2281 cgggagctgc gcgaccggct ggacatcgtc tcggccgagc gcatctccga cgagctttcg
     2341 aagttgctgg tggggtcccg gccctccaag ggcctggacc tggtggtgag caccggcctg
     2401 gccgagcgct tcttgccgga gctcccggcg ctcacggtgg agcaggaccc agtgcacaag
     2461 cacaaggacg tgctgcggca cacgtacgcc gtggtggagc ggtgcgagcc cgacctggtg
     2521 ctccgactgg ccgcgctcct gcacgacatc ggcaagccga agacccgccg gatcggggcc
     2581 gagggcgtgt ccttccacca tcacgaggtg gtgggggcca ggatggccag ggagcgcctg
     2641 gtggccctgc gctacccgtc ggccgtggtg gaggacgtgg ttcgcctggt ggagatgcac
     2701 ctgcgcttcc atggctactc cgagtggacg gacagcgcgg tgcggcggta cgtgcgtgag
     2761 gccggcccgc tcctggaccg gctgaaccag ctcacgaggg cggactgcac cacccgcaac
     2821 ccgttcaggg cccggcagct ggcggccctg caggacgacc tggaggagcg gatcgcccgc
     2881 ctgggcgaag aagagaacct gcaggcgatc cgcgcaccgc tggacggcaa ccaggtgatg
     2941 gagcacctgg gcctggcccc gggccccgag gtgggccggg cgctcgccta cctgctggac
     3001 gaacggatgg agcggggtcc catcgaggag gatgaggcct accggctgtt ggatgcctgg
     3061 gccaaggagc gggggatcgc tcgccggtcc tgagacccgc tcctcacccg gcgcgccggg
     3121 agctccggct ccggcgggcc ggggcgccga tgctcacccc aggccgccga ggtcggaagg
     3181 cggccagatg atcacgaagg ccttgccgat cacgtcgcgc tcgggaacgg gtccgagcgt
     3241 gaaccgcgag tccgcggagt tgcctcggtt gtcccccatc acgaacagct gaccgggcgg
     3301 cacccgctcg ggcccgaagt cctgcatgga cgccttggcc tgagcggtga ggtacggctc
     3361 ggcgatccgc tggccgtcca cgaacaccgc gtggcctcgg atctgcaccg tgtcccccgg
     3421 caggccgatc acccgcttga tcaggtcctc gtccggcggc tgcgccaccc cgatgccctc
     3481 cccgagccag tggatcacgc cctggaacca gttccggtcg gtggccgaga ccacctgggg
     3541 gttcgagaag acgaccacgt cgccccggtg gatatcgccg aaccggtagg ccagcttgtt
     3601 caccaggacc cggtcgcccg gcacgagcgt gggctccatg gaggccgaag ggatgaagaa
     3661 ggcctggacc aggaaggtct tgatcaagat cgccaccacc agggcgatga gcaccagcgc
     3721 cggcacctcg cggaggaagg aacctcggtc ctccctctcc ttcctgtgcc ttccgcgctg
     3781 gggctccgct cccgagacgc cctgccggtc gtcgccctcc atggtggtca gcccggaggg
     3841 ctgctcggga tccccgctcg acccctcgtc gggacggacg gcgtcgtcgg gcgcgggcag
     3901 ttcgtccgag atcgacatgc gggccagcct accgcctcgg ggccactacg gcgggaccac
     3961 ccgcacctcg acggctttga acgaggccca gacctcgtca ccggggcgca gccccaggcg
     4021 gtccagcgag cccgtggtga tctccgccac caccgggggg cgggaggcga tgtggacccg
     4081 cacccgttcc gcgtcggcgg ccacggcccc gacccgtccg tggacgacgt tgcgggcgga
     4141 gccctcgggg ggacgtgcat ggatcgcgac atccgcgggg cgcagcagcc cgaacacggt
     4201 ggtcaccgag ggatccaggt ctggcggcca accgaccacc acatcgccgt ccgaggtgcg
     4261 gatccggccg gctccgtcag gcatgcgctc gagccgaccc gagaaccagt tcaggccgac
     4321 cagatcggcc gcgtaactgc tccgggggcg atcgcggatc tgccgcggcg tccccgactg
     4381 cgtgacccgt ccctcctcca cgatcaccat ccggtcggcc agggtggtgg cttcgaccgg
     4441 atcgtgggtc accagcaacc gggctccggg gaacgagccg accacctcgc ggatcagggc
     4501 ccgcagttgc gctcgagccg agacgtccag ggccgacagc ggctcgtcca gcaggagcag
     4561 ccggggtccg tggatcaccg ctcgggccag ggccacgcgc tgcgcctgac cgcctgacag
     4621 cgaggccggg cgatccgcgt cgcgccctga caggcccagc cgggccagca gctcggacgc
     4681 gcgccgacgg gcctcgtccc tgggcacccc gcgagcccgc agggggaacg cggcgttctc
     4741 cacggcggac aggtgcggga acagcaaccg gtcctggaag accacgccga ccgggcgagc
     4801 ctcggggggc acgtggatcc gggcccggac gtcgtccagg acccgtccgt ccaggaccac
     4861 ggctccgtgc tcgggaggca gcagcccgga cagcacctcg accagggtgg acttgccggc
     4921 gccgttgggc ccgagcagcg ccacggtctc cccgtcctcg gcgtccagcc gcgcctgcac
     4981 cgcgaacccg ccccgccggg cccggacctc gacccgcagg ctcatggcgc ggacaggaac
     5041 cgggcccgca gccccacgat caccgccagc gagatggcca cgagggacag ggacagcgcc
     5101 acggccccgc ccgggtcggt ctgcagctcc tgaaacacgg ccaggggcag cgtctgtgtg
     5161 accccccgga ggttcccggc gaaggtgatc gtggcaccga actcgcccag cgcgcgggcc
     5221 cacgacagca cgaaccccgc cacgatctgg ggggccagga gcggcagcgt cacccgccgg
     5281 aggacgtacc agcgtgaggc tccgagcgtg accgccgcct gctccaggcg gaggtccatc
     5341 gctcgcaggc cggcctcgag cgccagcacc atgagcggaa acgacacgaa cgtggcggcc
     5401 aggatggcgg cgcccgtggt gaaggtgatc tgcacaccca acagccggta caggggggct
     5461 ccgaccagcc cgcgccgtcc gaacgccgtg agcagcgcga cgccgccgac gaccgggggc
     5521 atcaccaggg gcagcaccac gatcccgcgg aggatcgtgc gccccgggac ccgggaccgg
     5581 gccagcaccc aggccaaggg gaggcccacg acgaaggcga tcaccgacgc cgaggtcgcg
     5641 accaccagcg acagccgaag ggcggtgagg gccaccggcg aggccacggc ggcccatgcc
     5701 ttcgaccacg gggttcgcag ggccagcccg gccaggggca gggcgaccag cgccgcgccc
     5761 gccgcggcca ggacggcgat cgggagcggg gtgagccccc ggcggggcgt ccagctcatc
     5821 cggggggccc gaatccgtag cgtcgcagga cctcctgtcc ggggccgagg acgtagcgga
     5881 cgaacgcggc ggccaggtcg ggatggggac tgccgtcgac cacggcgatc gggtagtcgg
     5941 ccatcacgtt cagcgaaggg gggatgtcga tctgccgcac ccgcgatgcg acgtccggcg
     6001 tgacgtccgt ccggtacacg atcccggcgt ctgcctcccc cagcagcacc ttctgcacca
     6061 cccccttcac gtcgctctcg ttcgagacca cgttggccgt ggcggcggcc aggatgccgg
     6121 cgttccgcag gacctcccgc gcgtagtcgc cggccgggac gccctcggcg gccagcacca
     6181 gctccacgcc cggccgggtc agatcccgca gggaaacgat cccggccgga tcgtccgccg
     6241 gggtgatcac ggtcagcgag ttgcgcgcga agtcgccccg gtgatcgaca cccggaccct
     6301 gggtctggac ccggtccatc tcgaccgggc tggccgaggc gtacacgtcg cccggggctc
     6361 cctcgatgat ctgctcggcg aggccatcgg agggcccgaa gctgaaccgc acggtggaac
     6421 cggggtggcc ccgctcgaag tccctgccga tggcggtgaa gacctgggtg agcgaggccg
     6481 cggccagcac ggtcaggcgg ccgccggatg agctcgatcc gccggttcgg ccttcggccg
     6541 gcccgcagcc gaacagcccc acggccgcga tcagcgcgag cgcgacaaca gggccggagc
     6601 gacgcccccc ctcggcggag ctcaccggtc gacctcgatc atcacgttgg tcgccttcac
     6661 cgttgccacg gccgccatgc cgggggagag cccgatctcc tccaccgcct cccgggtgat
     6721 caaggcgagg atccggtgcg gtccagccag cagctcgacc gtcgccgcca ccccgtcggt
     6781 ctccacgtgg gtcacgatgc cggggaaacg gttgcgcacg ctcgccgacg tcacgcgcgg
     6841 ccgctcgcgg cgctcaccca ggagccgggc gacctcctcg gccggtaccc gacgctgtcc
     6901 gccctccgtg cgacgggtct ggagcttgcc ctgccgttcc caccgccgca gggtctccac
     6961 ccggaccccg agcagttcgg ccgcttctcc gatccgatac gaggcttcca tggggacact
     7021 gtacagggaa agctggatag gtaagtaccg agagccggcg ataccggagg atcaggtcgt
     7081 tgcgaggggt ccgggtggag aggtcgacgc gtggctcaga ccgcgaaccg aacatgcatc
     7141 acgtcgccct cgcggaccac gtagt
//
LOCUS       DASQQQ010000024        11581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_57449, whole genome shotgun sequence.
ACCESSION   DASQQQ010000024 DASQQQ010000000
VERSION     DASQQQ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11581
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57449"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VE646_01370"
     CDS             complement(<1..461)
                     /locus_tag="VE646_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZD78677.1"
                     /translation="MRIVSLLPSATEIVYALGLGDQLEAVTDECDFPAEAQHKPVVSR
                     SVLPQGRPLSSREIDAAVHERIDGDQPLYRLDTDLIRRIRPDVILTQDLCRVCAVPSG
                     RVQQALDQIGASEARVLSLEPKSLEQILESIEAVGRLLGVEERATEVVRLLR"
     gene            complement(458..1627)
                     /locus_tag="VE646_01375"
     CDS             complement(458..1627)
                     /locus_tag="VE646_01375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HZD78678.1"
                     /translation="MVERVFTLPDLGEGLEEAEIAGWLVSEGQAITLDQPLLEVETAK
                     ATVEIPSPRAGRVGKIHAEAGSTVKVGEPLVTFEVEEVPTETNVLAPSGMSSMVQVGD
                     EPAGPRAASRVPPATPAVRRLAKDLGVDLSAVAGSGPGGRITREDVEGAARGAGTSEP
                     SLTPEDVEVVPISAIRRTIAENLTRVVRDVPLVTTFRTLDCTSLEAFRRDLGVSPLPV
                     VARALAEVCGAHPYLNASYRAEEGRIELFQSVHMGIATDTERGLVVPVIRDVASLGLG
                     AVAEEIARLAVLARDGRLSPTDMRGGTITITNTGSYGSEWGTPIPTPGQGAIVALGVI
                     QPRALVVDGRVQARPSCTLSLTFDHRLLDGATAGRAFGDLVDLLQDRERLERLPR"
     gene            complement(1627..2616)
                     /locus_tag="VE646_01380"
     CDS             complement(1627..2616)
                     /locus_tag="VE646_01380"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZD78679.1"
                     /translation="MGEHPVGEHTMVAALNAALRDALASDARTLVFGEDVADLGGVFR
                     VTEGLRREFGQERVFNTPIAEAGIAGICVGLALAGWRPIAELQFDGFSYPALDQVISH
                     LAKYRTRTRGRVPMPVVIRIPSFGGIRGKEHHGESPETYYVHTAGLKVVVPSSPLDAY
                     RLLSRSIRDPDPVIFLEPKSRYWAKETGELTADGPGIGTARTLREGDACVIVSYGAMV
                     TRCLEAADRLQEDGTTCRVIDLRSLVPLDTAAIGDAVRATGRAIVVHEAPRTLGMGAE
                     VAARIMEEAFDSLEAPVARVAAPDVPYPPAALENAYLPSVERICDAVRRTVAY"
     gene            complement(2609..3667)
                     /locus_tag="VE646_01385"
     CDS             complement(2609..3667)
                     /locus_tag="VE646_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HZD78680.1"
                     /translation="MSTRERVLDEPFLQVIAPDGTAVGEPDPALSVEDLRELFRLMVR
                     ARHLDRGCLALQRQGELTVYPPFEGQEASQVGSAFALSGEDFVFPSFRELAAAITRGV
                     DVLEYLQYHRGTWHGGAYDPFRAGFAPICVPVATQIVHAVGWALGAKLDHRRACSLAY
                     FGDGSTSEGDFHEAANIAAVFQAPIVLFCQNNGWAISVPLEEQLAGPIWRRAEGYGFP
                     GVRVDGNDVLAVHRATKEAADRARHGDGPTLIEAITYRMGPHSTADDPSRYRTREAVA
                     AWRGRDPIERFRRYLLHRQLVDEHLVSTIEEEARTWVRGVREELVGLAPPPAQELSAF
                     AFERPPEGLGPVAGSTGG"
     gene            3690..4526
                     /locus_tag="VE646_01390"
     CDS             3690..4526
                     /locus_tag="VE646_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78681.1"
                     /translation="MDRREHIHCSLIVVPCAELRVFQPLDAFPAEERARWERYIVEAR
                     HVGPGRGRYRQRVTPEGLGIMVEAGEGAEVRLVGGTYFVCPWRTRLRVLAGILAFREA
                     APFEGSEGFIPKRLARRAGKELSRLRRRDPAAISCIMQSPWHVPVRWFVLFSDAERRL
                     AEEDGRFRLRYLSTVKRARRRAERAIPVLRNTDLGPIAGVIVELVEWLSAFDPRAVVE
                     LDYGGLCDLMTWDELDDDHSARDIQEAIGALSSDQIPRSAQLYRSLVGRWAEVRSHEA
                     LN"
     gene            complement(4538..4610)
                     /locus_tag="VE646_01395"
     tRNA            complement(4538..4610)
                     /locus_tag="VE646_01395"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4575..4577),aa:Phe,seq:gaa)
     gene            5399..6376
                     /locus_tag="VE646_01400"
     CDS             5399..6376
                     /locus_tag="VE646_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78682.1"
                     /translation="MTYVGLLITVAAVFVLPLPPIASVGLAIFGSLLTMLEGRSIYRL
                     FAGALVAWSLFALLLTGPALRHAPDLQEPVPPPSGYGFKLDPGATNREHTYASQPIPA
                     QGAASAAVEVVESYVKRLAPGWTVISRADVPRNVEVQLRQGDTSRGIQVSVSVFMPRG
                     RPAVLVLHIHTILCGEDGPGVASGEVGCWAAPLARLVGYPDGGPVSNTPEPSAGPFRE
                     PVPLPPNYGFVPRNSTEEVHVYESTTAMSFHEANRAQRSVLRYYRRSLAQWTIVEQDR
                     ADLLLKAPDSTDGLAIDSTWTAMNGGGVVTLQIRAISCQTDYYCHWSPV"
     gene            complement(6554..6632)
                     /locus_tag="VE646_01405"
     tRNA            complement(6554..6632)
                     /locus_tag="VE646_01405"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6594..6596),aa:Asp,seq:gtc)
     gene            complement(6654..6726)
                     /locus_tag="VE646_01410"
     tRNA            complement(6654..6726)
                     /locus_tag="VE646_01410"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6690..6692),aa:Glu,seq:ttc)
     gene            6918..7094
                     /locus_tag="VE646_01415"
     CDS             6918..7094
                     /locus_tag="VE646_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78683.1"
                     /translation="MELVVRALLEFLGIVERGATRREPVALPAWTRLIPPIAAGVLTL
                     ASFLLFLVIRALVG"
     gene            7504..8385
                     /locus_tag="VE646_01420"
     CDS             7504..8385
                     /locus_tag="VE646_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZD78684.1"
                     /translation="MLSLALALISGLAWGSADFVGGVMTKRLAPATVLVVSQAAGLTF
                     IGVLVVASGDPVPPSRSLVYGALGGLAGAIGLAALYKGLAVGRMSIVAPTAALSGAVP
                     VIVGFLQSERPALLQLVGMAVAGVGVLVAVRTPDPRAVLPGDASETAGRSPRAGMTRG
                     IGYALGAALFLGLFVTSLDAAGDASAVWASLMIRAVSVPLFFAAWLLMRSDRRRPTVS
                     ELWLLVVVGMLDNGANVLFALAADRGLLTLVAVLGSLYPVSTVLLARAFLRERLTGTQ
                     AAGVVAAFTGVALIAVG"
     gene            8494..10392
                     /locus_tag="VE646_01425"
     CDS             8494..10392
                     /locus_tag="VE646_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HZD78685.1"
                     /translation="MEVIPAKRPAIRTAIRARVVPSAILSLVITLVAPTVSAASTGTV
                     GAGYAGSSMRASDPSRMVATSPPPGAVPGIDVSHWQGQIDWPKVAASGQRFVIAKATE
                     GVNYIDPTYATNRAGAMAAGLVFGAYHFARPDLHPNNPIGEADHFVDVAQPSAGNVVP
                     VLDLERSGTLTQAQLTQWILQWLGEVTARTGIRPMVYTSPNGWASRTGDTTAVADAGY
                     TLLWVAHWFVLEPQLPANDWEGNGWAFWQYDDCGSVPGIQGCVDMDWYRSADFTPATV
                     PSPDTVAPVASLATPTGVAGPVTVSFSEIVNGVTRTNVTLSGLGSGPVPSALGCWSKR
                     APVDCAVGNVITAVLTPIDPLVPGEGYAAEVNPPGTTPVADRAGNPAIPVEQTFALPT
                     EVEQGSPAVRYAWRSVGAPKAYGGSFAVERSTGATASFPFTGNSVTWYTATGPNQGKA
                     VVSIDGRGRGTFDQFAPSWRVRVARTFNRLGPGRHTITIRVLGSSSSAATDTQVVVDA
                     FGLASGVVKQPQLVVSWGRVRMSAASGGTVAIGDLRRSAALFSFWGSGVEWRTVLGPD
                     QGRAALYLDGMLVRTVDNFAPRASFQVRSLTGLSVGLHQLRVEPLGTSRPRSGGAAVV
                     IDSLTALP"
     gene            10478..>11581
                     /locus_tag="VE646_01430"
     CDS             10478..>11581
                     /locus_tag="VE646_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HZD78686.1"
                     /translation="MSERITQVDLARDAAASGAWRDAYRLLSSIDGSGLGAEDLEHLA
                     DAAWWLSRFDESFEVRRRAYASFAASGDDLGAGRAATRLCIEHFLRQEAAVAGGWLKR
                     AERHLHDLPEASEQGFLAVVQATVARFGGDLNAASRLSQRATQIGRRLADRDLVAMGI
                     HTEGLVRIAAGDVEEGVALLDEAMTSVVAGELSDYFTGAVYCNVLDACLGLADLRRAG
                     EWNDAARAWCESLPPDSPYPGFCRVNRAEVAALRGSWSEAEAEAMRAAEELMRVNPMN
                     AAPAFYEAGEIRRRMGDAGGAEEAFARARELGLEPQPGLALLRLQQGKVEAAGRGLKT
                     ASAAAAGSPLRRATLLGAMVEVALAAGDLDAATT"
BASE COUNT         1637 a         4025 c         4144 g         1775 t
ORIGIN      
        1 cggagcaatc ggaccacctc ggtcgcgcgc tcctcgacgc ccagcagccg gcccaccgcc
       61 tcgatgctct ccaggatctg ctccagcgac ttcggctcca gcgagagcac ccgggcttcc
      121 gaggcgccga tctggtccag ggcctgctgc acccgtccgc tcggcacggc gcacacccgg
      181 cataggtcct gcgtgaggat cacgtccggt ctgatccgcc ggatcaggtc cgtgtccaga
      241 cggtacaggg gctggtcgcc gtcgatccgt tcgtgcaccg ccgcgtcgat ctcgcgggag
      301 ctcagcggcc gaccctgggg cagcaccgac cgggagacca ccggcttgtg ctgagcctct
      361 gccgggaagt cgcactcgtc ggtcaccgcc tccagctggt cgcccagccc gagcgcgtag
      421 acgatctcgg tggcggaggg cagcagcgag acgatcctca tcgcggtaga cgctccagtc
      481 gttcccggtc ctgcaggagg tccaccaggt cgccgaacgc ccgtccggcg gtggcgccat
      541 ccagcaggcg gtggtcgaac gtcagcgaga gcgtacagga gggccgggcc tggacgcgcc
      601 cgtctaccac cagtgcccgg ggctggatca cccccagggc aacgatcgct ccctgtccgg
      661 gggtggggat cggcgtcccc cactcggatc cgtagctacc cgtgttcgtg atggtgatcg
      721 tgccgccgcg catgtccgtc ggcgagagcc gcccgtcgcg ggccaggacg gccagccggg
      781 cgatctcctc cgccacggcg ccgagcccga gcgaagccac gtcccggatc accggcacca
      841 ccagcccgcg ttcggtgtcg gtggcgatgc ccatgtggac cgattggaac agctcgatcc
      901 ggccctcctc ggcccggtac gaggcgttga ggtagggatg ggcaccgcac acttcggcta
      961 gggcgcgcgc caccaccggc aggggggaca caccgaggtc gcggcggaac gcctcgaggc
     1021 tcgtgcagtc gagcgtccgg aacgtggtga ccaagggcac atcccgcacc acgcgggtga
     1081 ggttctcggc gatggtgcgg cggatcgccg agatcggcac cacctccacg tcctccggcg
     1141 tgagggaagg ctccgaggtc cccgcccccc gggccgcgcc ttccacgtcc tcccgggtga
     1201 tccgacctcc cgggccactt ccggccacgg ccgacaggtc gaccccgagg tccttggcga
     1261 gccttcgcac ggcgggggtg gcggggggaa ctcgggacgc tgctcggggg ccggccggtt
     1321 catcgcccac ctggaccatg gaggacatgc cgctgggagc gaggacgttc gtctccgtcg
     1381 gtacctcctc cacctcgaac gtcaccagcg gctcgcccac cttcaccgtg gagccggcct
     1441 cggcgtggat cttccccacg cgaccggccc gcggtgaggg gatctcgacg gtcgccttgg
     1501 ccgtctcgac ctcgagcagc ggctggtcca gcgtgatcgc ctggccctcc gagaccagcc
     1561 agccggcgat ctccgcctcc tcgaggccct cgccgaggtc gggcaacgtg aagacccgct
     1621 cgaccatcaa tacgccaccg tccggcggac ggcatcgcag atccgctcca cgctcggcag
     1681 gtaggcgttc tccagcgcgg ccggcgggta ggggacatct ggcgccgcca cccgggcgac
     1741 cggcgcctcc agcgagtcga acgcctcctc catgatccgg gccgcgacct ctgcccccat
     1801 ccccagcgtg cgaggcgcct cgtggaccac gatcgctcga ccggtggccc ggacggcatc
     1861 cccgatggcc gcggtgtcca acgggaccag ggaccgcagg tcgatcaccc ggcaggtcgt
     1921 gccgtcctcc tggagccggt ctgccgcctc caggcagcgc gtcaccatgg cgccgtagga
     1981 cacgatcaca caggcatcgc cctcgcggag ggtcctggcg gtgccgatgc caggaccgtc
     2041 ggcggtgagc tcgccggtct ctttggccca gtaccggctc ttcggctcca ggaagatcac
     2101 cggatcgggg tcccggatcg agcgggacag gagccggtac gcatcgagtg ggctcgaggg
     2161 caccaccacc ttcaggccgg ccgtgtgcac gtagtaggtc tccggactct cgccgtggtg
     2221 ctccttgccc cggatcccgc cgaacgacgg gatccggatc accacgggca tcggaacgcg
     2281 cccccgggtc cgggtccggt acttggccag gtggctgatc acctggtcca gggcggggta
     2341 gctgaagcca tcgaactgga gctcggcgat gggcctccag ccggccaggg ccaggcccac
     2401 gcagatcccc gcgatgccgg cctccgcgat cggcgtgttg aacacgcgct cctgaccgaa
     2461 ttcgcgtcga aggccctcgg tcacccggaa cacgccgccc agatcggcga cgtcctcacc
     2521 gaagaccagc gtgcgggcgt ccgaggccaa cgcgtcgcgc agggccgcgt tcagggcggc
     2581 gaccatcgtg tgctcgccca ccgggtgctc acccaccggt gctccctgcg accgggccga
     2641 gaccctccgg gggacgctcg aacgcgaacg ccgagagctc ctgtgccggg ggtggggcca
     2701 gcccgaccag ctcctccctc acccctcgga cccaggtccg agcctcctcc tcgatcgtcg
     2761 agacgaggtg ctcgtcaacg agctgccggt ggaggaggta gcggcggaac cgttcgatcg
     2821 gatcccgccc tcgccaggcc gcgaccgctt cccgggtgcg gtagcgggag gggtcgtccg
     2881 cggtggagtg tgggcccatt cggtaggtga tcgcctcgat gagcgtgggg ccatcgccgt
     2941 ggcgggcgcg atccgccgcc tccttcgtcg cccgatgcac ggccagcacg tcgttgccgt
     3001 ccacccggac gccggggaac ccgtagccct ccgcccggcg ccagatggga ccggccagct
     3061 gttcctcgag cggcaccgag atggcccagc cgttgttctg gcagaacagc acgatcgggg
     3121 cttggaagac ggccgcgatg ttcgccgctt cgtggaaatc cccctcggag gtcgagccgt
     3181 ccccgaagta ggccagggaa cacgcgcggc ggtgatcgag cttggccccg agcgcccagc
     3241 ccacggcatg cacgatctgc gtggccaccg ggacgcagat cggggcgaag ccggcccgga
     3301 acggatcgta ggccccgccg tgccaggtgc cacggtggta ctggaggtac tcgagcacgt
     3361 cgacgccccg ggtgatggcc gccgcgagct cccggaacga ggggaacacg aagtcctccc
     3421 cggacagggc gaaggcgctg cccacctggg aggcctcttg cccctcgaac ggcgggtaga
     3481 cggtgagctc gccctggcgc tgcagggcca agcagccccg gtccaggtgc ctggcccgaa
     3541 ccatgagccg gaagagctcg cgaagatcct ccacgctcag ggccgggtcg ggctcgccca
     3601 ccgccgtgcc gtccggggcg atgacctgca agaacggctc gtccagcact cgttcgcgcg
     3661 tgctcatctc cacaggaccc tcgaccacgg tggatcggcg agagcatata cactgctccc
     3721 ttatcgtggt cccctgcgcc gagctccgcg tgttccagcc cctcgacgcg ttccctgcgg
     3781 aggaacgcgc ccgatgggag cggtacatcg tggaggcgcg ccacgtcgga cctgggaggg
     3841 ggcgctaccg ccagcgggtc acgccggagg gcttgggcat catggtcgag gcgggagagg
     3901 gggccgaggt ccgcctggtg ggcggtacct acttcgtgtg tccctggcgg acccggctca
     3961 gggtgctggc ggggatcctg gccttccgcg aagcggcccc cttcgagggc agcgagggat
     4021 tcatcccgaa gcgcctggca cgccgtgcag gcaaggagct gagccgtctc cgccgaaggg
     4081 atccggcggc gatctcctgc atcatgcaga gcccttggca cgtgccggtc cggtggttcg
     4141 tccttttctc ggatgccgaa cggcgattgg ccgaggaaga cggccgtttc cggctccgct
     4201 acctaagcac ggtcaagcga gcgaggcggc gggccgagcg tgccatcccg gtcctgcgga
     4261 acacggacct gggcccgatc gccggtgtca tcgtcgagct ggtcgagtgg ctctcggctt
     4321 tcgaccctcg ggccgtcgtg gagctcgact acggtgggct gtgcgacctc atgacctggg
     4381 acgagctgga cgacgatcac tccgcccggg acatccagga ggcgatcggg gccctttcct
     4441 cggaccagat cccccgctcg gcgcagctgt accggtcact ggtggggcgt tgggccgagg
     4501 tccggagcca cgaagcgctg aactgagcga ggagcggtgg ccaggggcag gatcgaactg
     4561 ccgacaccgc ggttttcagc cgcgtgctct gccaactgag ctacctggcc cggagtgggg
     4621 tcggccgagg ggccgacacc cgcccgttgc gcgcccgacc ggcttcgatc ccgcgatctc
     4681 cgcttgacga tccgggcgct cgcgttctga aggataccga ctcatcccgc cgtgtagcgt
     4741 ttgcccagct agggcggcga atcggctgcc gagtcgtcct tgttcgtcct gccgtgtccc
     4801 gggagtgaca gcaacaggtg gctgcaagtc ggcctcgccc ctcgtctccc agcgcggttt
     4861 cgatggcgga tgcggccttc ccctgggagg ccctccgttc cgcgatcgcg gccgggaggt
     4921 gtggagggcc gactcgaccc accttcgggc ccgacctcct ggccacttgc tcacgttgca
     4981 gccgccgacc tgccgatgga aacgtccgac ggcccttccg cgcatcagac tcccgattag
     5041 tcaccccgcc agatgaaggt taggcccgtc aaggcgatga agatcccgga gacgactgcc
     5101 gagcctcgaa gccaaggcac gtcgcgcgct cgcacctggt cgatcgtgcg caacaactgt
     5161 cgtgcatact agcggttgaa caccatgaac atgacgccga ggacgatgag cggcacgccg
     5221 agtacctgca tgtgcgcagg gtagcggacc gcgcggcaac ggtctcgacc tggagccgtt
     5281 tgcaggaccc cgggaggttc cgttgaggac gcgactcctc aagctcggcc ggcttcccgc
     5341 cgatgggcgt gtgagcgggg tgttcatggc taggagcttg gtcgaccgaa actcgcccat
     5401 gacctacgtc ggcctgctca tcaccgtcgc cgccgtgttc gtgctgccgc tgccacccat
     5461 cgcctcggtc gggctcgcca tcttcggatc cctcctcacg atgttggagg ggcgcagcat
     5521 ctaccgcctg ttcgccggcg cgctggtcgc ttggtcgctg ttcgcgctgt tgctgaccgg
     5581 cccggcgctg cgtcacgcac cggatctgca ggagcccgtc ccacccccgt ccggatacgg
     5641 cttcaagctg gatcccggcg caacgaaccg cgagcacacc tacgcgtcgc agccgatccc
     5701 agctcaggga gccgcatccg ccgccgtcga ggtcgtcgag tcctacgtca agcgtctcgc
     5761 gccgggatgg accgtgatct ctcgcgcgga tgtgcctcgg aacgtggagg tccagttgag
     5821 gcagggcgat acctcccggg ggatccaagt ctcggtgtcc gtcttcatgc cgcgaggccg
     5881 gccggccgtc ctggtccttc acatccatac catcctgtgc ggggaggatg gtcccggtgt
     5941 tgcctccggg gaagtcgggt gctgggccgc gcctctcgcc aggctcgtcg gctatccaga
     6001 cgggggacca gtctcgaaca cacccgagcc aagcgccggg ccgttccgcg agccggtgcc
     6061 cttgccgccc aactacgggt tcgttccgcg gaactcgacg gaagaagtgc acgtgtacga
     6121 gtcgaccacc gccatgagct tccatgaggc aaatcgcgcg cagcgatccg tgctccggta
     6181 ctaccggcgg tcgctcgccc agtggacgat cgtggagcag gatagggcgg atctccttct
     6241 caaggccccc gattcgacgg acgggctcgc gatcgattcc acctggactg cgatgaacgg
     6301 aggcggcgtg gtgaccctcc agatccgggc gatctcgtgt cagacggatt actactgcca
     6361 ctggagccct gtgtgagcct gactcgcttc tgagtctgag acggaggagt tgagcgcttc
     6421 gtgtggttcc gaccccaaga ggacagcgca ggcgctcacc atggaggtgt acgcgctgtg
     6481 cctgtgggtt tgaagaccgg tggcagcaag aagggccgcc gaacgctcga cggccccaaa
     6541 cttgctggtc agatggcgcg cccgacgggc ttcgatcccg cgatctccgc cttgacaggg
     6601 cggtgaggac ggccggactc ctctacgggc gcgtgtcgat atgcggttgt cggtgccccc
     6661 aacgggattc gaacccgtgt cgccgccttg aaagggcgaa atcctaggcc gctagacgat
     6721 gggggcccgg gcgcccgggg acgccggtcc cgcccctggg ggcaaccgag gaggaagtat
     6781 agccgccccg agcccggccc cgctccgcgc ttcgagtcat gcccgcatcg cccacgcaac
     6841 cacccgcggg ccggggccgg gtgccgacca tcgtcggttc ctgtcgagcg ccccttcagg
     6901 tcggggccgg aacaccgatg gagcttgtgg tgcgagcgct gctggagttc ctggggatcg
     6961 tggagcgggg ggcgacccgg cgggagcccg tggcgctgcc ggcgtggacc cgcctcatcc
     7021 cgccgatcgc cgccggcgtg ctgacgctgg cctcgttcct cctcttcctc gtgatccggg
     7081 cgctggtcgg ctgatcgggc cctggagccg gaaggcccct cccacgcgga gctcgtcgtg
     7141 gacggagcac tccagcacgt ggcggatgcc gcgtctgggg aggaccaggc gcccgggttc
     7201 catccggaac gcgcgcacca cgcggaatcg atggtccagg agcgcgacga ggatcgggaa
     7261 ggccatcccg aacgtgtacc gaccgcgtcc ggggaagcag caggcctcga gcgcatccgg
     7321 gctcgggcaa gccgagtagc ccgcggacgc gctcgcgccg cgtctggggc acgtccagat
     7381 gccagcgccg tccctcgtca tcgatcgcca gcacggatct cacgccgggg gacgatacgg
     7441 catcccgccg acacggtggt catcccgaga ggcccgcgtg ggccacgccg gtagccttgc
     7501 cccatgctgt cacttgcgct ggcgctcatt tccggtctgg cctggggctc ggccgatttc
     7561 gtgggcggcg tcatgaccaa gcgcctggcc cccgccacgg tgctggtggt gagtcaggcc
     7621 gcgggactga ccttcatcgg cgtgctcgtg gttgcctccg gagacccggt tccgccatcg
     7681 cgatcgctgg tctacggggc cctgggcggg ctggccggag cgatcggact ggcggccctg
     7741 tacaagggac tggcggttgg caggatgagc atcgtcgcgc cgaccgcggc cctgtcgggc
     7801 gccgtccccg tcatcgtcgg cttcctccag agcgagcgtc cggcgctcct ccagctggtg
     7861 gggatggcgg tcgccggggt aggcgtcctg gtggccgtcc ggacacccga tccgagggcg
     7921 gtcctcccgg gcgatgcctc ggagacggct ggccgttcgc cgcgagctgg catgacgagg
     7981 ggcatcggct atgccctggg ggccgcgctg ttcctgggcc tgttcgtcac ctccctcgat
     8041 gccgcgggag acgcgagcgc ggtctgggcc agcctgatga tccgggcggt gtcggtgccc
     8101 ctcttcttcg cggcttggct gctgatgcgg agcgatcgtc gacgccccac ggtctccgag
     8161 ctctggctcc tcgtggtcgt cgggatgctc gacaacggcg cgaacgtgct gttcgcactg
     8221 gcggccgacc gtgggctcct gacgctggtc gccgtgctcg gttccctgta ccccgtgtcc
     8281 acggtgctcc tggccagggc gttcctgcgg gagcgactca cggggacgca ggcagcgggc
     8341 gtggtggcgg cgttcacagg ggtcgcgctg atcgccgtcg gctgacggcc gggcgggctc
     8401 acgccatcga ccggcgcaac ctccaggagc cggcgacgtt gtcagggacg gggttgtccg
     8461 catggtcgac ccccggcgca catcgccctg accatggagg tgatcccggc gaaacgacca
     8521 gcgatccgca ccgcgatccg agcgagggtg gtcccctcgg ccatcctcag cctcgtgatc
     8581 accctggtcg ccccgaccgt gtctgcggca tccacgggaa ccgtgggggc cggctacgcg
     8641 gggtcctcga tgcgcgcgtc ggacccgtcg cggatggtgg cgacctcgcc gcccccgggg
     8701 gcggtgccgg ggatcgacgt ctcgcactgg caggggcaga tcgactggcc gaaggttgcg
     8761 gcgtcggggc agcggttcgt catcgcgaaa gccaccgagg gcgtcaacta catcgatccc
     8821 acctacgcaa cgaaccgggc cggtgccatg gctgcggggc tggtgttcgg cgcgtaccac
     8881 ttcgcccgcc ccgatctaca ccccaacaac ccgatcggcg aggccgacca cttcgtcgac
     8941 gtggcgcagc ccagcgccgg gaacgtcgtg ccggtgctgg acctcgagcg atcggggacg
     9001 ctcacccagg cgcagctcac ccagtggatc ctgcagtggc tcggcgaggt cacggccagg
     9061 accggcattc gacccatggt ctacacgagc ccgaacggtt gggccagccg caccggtgac
     9121 acgaccgcgg tcgccgacgc cgggtacacg ctgctgtggg tcgcgcactg gttcgtgctc
     9181 gagccgcagc tgccggccaa cgactgggag ggcaacggct gggccttctg gcagtacgac
     9241 gactgcgggt cggtgccggg gatccaagga tgcgtggaca tggactggta ccggtcggcc
     9301 gacttcacgc cggccaccgt cccctcgccg gacaccgtcg cgccggtcgc ctcgctggcc
     9361 acgccaaccg gggtggcagg accggtcacc gtctcgttca gcgagatcgt gaacggagtc
     9421 accaggacga acgtcacgct gtccgggctg gggtctggac cggtgccctc tgcgctgggc
     9481 tgctggtcca agagggcccc ggtggattgc gccgtcggca acgtgatcac cgcggtgctg
     9541 acaccgatcg atccgctcgt gcccggtgag gggtacgcgg cggaggtgaa cccgcctggg
     9601 accacgccgg tcgccgaccg agccgggaac ccggcgatac cggtcgagca gacgttcgca
     9661 cttcccaccg aggtcgagca gggaagcccg gcggtcaggt acgcatggcg ctcggtcggg
     9721 gcgccgaagg cgtacggggg ctccttcgcc gtcgagcgct cgacgggggc gacggcctcg
     9781 ttccccttca cgggcaactc cgtcacctgg tacacggcga cgggcccgaa ccaggggaag
     9841 gctgtggtct cgatcgacgg tcggggccga gggacgttcg accagttcgc cccctcatgg
     9901 cgggtccggg tggcccggac gttcaaccgg ctggggcccg gccggcacac catcacgatc
     9961 cgggtgctgg gctcgagctc gagcgcggcc acggacacgc aagtggtggt ggatgccttc
    10021 ggcctggcct ccggtgtggt gaagcagccc cagctcgtcg tgtcgtgggg acgcgtccgg
    10081 atgtccgccg cctccggtgg cacggtggcg atcggcgatc tccggcggtc ggccgccctc
    10141 ttctcgttct ggggctccgg ggtggagtgg cggaccgtcc tggggcccga ccaggggcgg
    10201 gcggcgctgt acctggacgg catgctcgtg aggacggtcg acaacttcgc gccgcgggcg
    10261 tccttccagg tgcgatcgct gaccgggctg agcgtcggcc tgcaccagct gcgcgtcgag
    10321 ccgctcggca cgtcgcggcc caggtccggt ggcgccgcgg tcgtcatcga ctccctcacc
    10381 gcgttgccct gagccgtccg ggtcgaggcg gaagcaggat ccgacccgat aggctaggcg
    10441 gatccgcgag cgtcaccctg gagacgatgg tggagcgatg agtgagcgga tcacccaggt
    10501 cgatctggcc cgcgacgcgg ccgcgagcgg cgcatggagg gatgcgtatc ggctgctgag
    10561 ctcgatcgac gggtcggggc tcggtgcgga ggacctggag cacctggccg acgcggcgtg
    10621 gtggctctcg cggttcgacg agtcgttcga ggtgcgacgc cgggcatacg catcgttcgc
    10681 ggcctccggg gacgatctcg gagcgggacg agccgccacg cgcttgtgca tcgagcactt
    10741 cctccgacag gaggcggcgg tcgccggggg gtggctgaag cgggcggagc ggcacctaca
    10801 cgatctgccg gaggcgagtg agcagggctt cctggccgtg gtccaggcta ccgtggcacg
    10861 cttcggcggc gacctgaacg ccgcatcccg cctctcgcag cgcgccacgc agatcgggcg
    10921 ccggctggcc gatcgcgatc tggtcgcgat ggggatccat acggagggac tggtgcggat
    10981 cgcggccggc gacgtggagg agggcgttgc gctgctggat gaggccatga catcggtggt
    11041 ggccggtgag ctcagcgact acttcacggg tgccgtgtac tgcaacgtcc tcgacgcctg
    11101 tctggggctg gccgatcttc ggcgggcggg ggagtggaac gatgctgccc gggcctggtg
    11161 cgagtccctg ccacccgact ccccgtaccc gggcttctgc cgggtgaacc gggccgaggt
    11221 ggccgccctg cgtggctcgt ggtcggaggc cgaagcggag gcgatgcgtg ctgccgagga
    11281 gctgatgcgg gtgaacccga tgaacgccgc accggcgttc tacgaggcgg gtgagatccg
    11341 gcggcggatg ggtgacgcag ggggggcgga agaggccttc gcgcgggcga gagagctcgg
    11401 gctggagcca cagcccggcc ttgccctgct ccgtctgcag caaggcaagg tggaggccgc
    11461 ggggcgaggt ctcaagacgg cctccgcggc ggccgccggg agcccgctgc gacgggcgac
    11521 cctgttgggg gccatggtcg aggtcgcgct cgccgccgga gacctcgacg ccgcaacgac
    11581 g
//
LOCUS       DASQQQ010000025         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_29953, whole genome shotgun sequence.
ACCESSION   DASQQQ010000025 DASQQQ010000000
VERSION     DASQQQ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29953"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VE646_01435"
     CDS             complement(<1..844)
                     /locus_tag="VE646_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78687.1"
                     /translation="MVQADAAPSAPALPGSSAERRLLAAACVVGLALRLPWLLAGAQK
                     LLGHHGSDDLFYYTQVAHHLATGGGLSFDGLHATSGVQPLFALILVPFAPAFEGRPML
                     ACRVVLWSATAFTLATAWVLPSMGQALLGGARRGRWVGVVAGCLWVLHPRVLDTTFQG
                     TEAALAALCWVLSLRAWAGGASAIGLGATLGAGILARVDHLVLGAQTVLRRGPALFVM
                     LAPLAAWMVVATFSTGSPVQDSGAAKRLHGERIFALEHGLDVATPPAFAAPASRLADL
                     AEGVA"
     gene            1289..1594
                     /locus_tag="VE646_01440"
     CDS             1289..1594
                     /locus_tag="VE646_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78688.1"
                     /translation="MSATRDAVGRTLELGVDPFTVIGIAPPGLDAAFRTHPDLNKVHA
                     RANVDNTASQGVMEKAGMVQEGVLRQNRVERGETIDEAWFGVLRGEWTEAREGARRG"
     gene            1647..>2283
                     /locus_tag="VE646_01445"
     CDS             1647..>2283
                     /locus_tag="VE646_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78689.1"
                     /translation="MRTLRPTRSRLTAAAGTAAWLLAAPAALTGQVPGADSTKALEEV
                     RELQRAFESFRESHTPIQRGARPEGACDERIGRICIWFGGEDEETFPAELPDVALART
                     KLIGALEDAFERTPHRWVIGQLVYYLVESRNVDEAERVASRCGIPETWWCSALLGYVH
                     HMRREYIEAEAAFREALASMPVPLRDEWMTRYVLTGDVLEELEEASPTERDR"
BASE COUNT          375 a          825 c          808 g          275 t
ORIGIN      
        1 atgcgacgcc ctccgcgagg tcggcgaggc gggaggccgg ggcggcaaac gccggagggg
       61 tcgccacgtc gagcccgtgc tcgagggcga agatgcgctc tccgtggagg cgtttcgccg
      121 cgcccgaatc ctggaccggc gagccggtgc tgaaggtcgc caccaccatc caggcggcga
      181 gcggggcgag catcacgaac agggcagggc cgcggcgcag gaccgtctga gcccccagca
      241 ccagatgatc cacccgcgcc aggattccgg cgcccagggt cgcacccagc ccgatcgccg
      301 aggcaccccc ggcccatgct cgcagagaca gcacccagca gagcgccgcg agcgcggcct
      361 cggtaccctg gaaggtcgtg tcgagcaccc gcggatgcag gacccacagg caccctgcaa
      421 ccactcccac ccatcggccg cgccgcgccc cgcccaacag cgcctgaccc atcgacggca
      481 gcacccaggc cgtcgccagc gtgaacgccg tagccgacca gagcacgacc ctgcacgcca
      541 gcatcggccg gccctcgaag gcaggggcga acggcaccag gatcagtgcg aagagcggtt
      601 gcacgccgct ggtggcgtgg agaccgtcga agctcaggcc ccccccggtg gccagatggt
      661 gggcgacctg cgtgtagtag aagagatcat ccgagccgtg gtgcccgagc agcttctggg
      721 cgccggccag cagccacggc agacgcaggg ccagccccac gacgcaggcc gccgcgagga
      781 ggcgacgctc ggcggaagag cccggcaacg ccggggcgga gggagcggcg tcggcctgaa
      841 ccatcacctc gtaatgcaga cccaggagga cgggagcatc atgacaaccc caagcgctcc
      901 cggagccggc ggatggaccc tgcctcgagc ggaggggtca tcgtccggcc tcgctcctcg
      961 atcccggcca ggaggacctc caccttctcc cgcccggccg gcgtccacac ggcccgcagg
     1021 ggagagcctc gagtcgcgcc gcgctctcgg ccgagttcac ctcggctcgc agctcgcggc
     1081 cctcgatctc gatgtggcca tggacggtgt catcccacgc ggcgtgcatc gggttgcccg
     1141 gcaccgtcca gaccttcctg ccgctcccgc aggggcaggg agcgtttcgt gagactccca
     1201 tctccgatcc tctcctccgc tggtgcgttt ccggtggacc aatatagcct tgcgccggtc
     1261 cgcgccctgg acagagcacg ccacggcgat gagcgccacc cgtgacgccg tgggcaggac
     1321 cctggagctg ggggtcgacc ccttcacggt gatcggcatc gccccgccgg gcctcgacgc
     1381 ggccttccgg acgcaccccg acctcaacaa ggtccacgcg agggcgaacg tggacaacac
     1441 cgcttcgcaa ggggtgatgg agaaggccgg catggtccag gagggcgtcc tccgccagaa
     1501 tcgggtcgag cgcggcgaaa cgatcgacga agcctggttc ggcgtcctcc gcggtgaatg
     1561 gaccgaggcg cgggagggtg cccgaagggg atgagccccg tcgaagggtc ggggaaccgc
     1621 gaccccgccc cgcggctact accgtcatgc gcacgctcag acccacgagg tcccgcctca
     1681 ccgccgcggc cgggacggcg gcctggctcc tggccgctcc ggccgccctc acgggccagg
     1741 tgcccggcgc ggactccacg aaggccctcg aggaggtacg tgagctgcag cgggcgttcg
     1801 agagcttccg ggagagccac acgcccatcc agcgcggtgc acggcccgag ggtgcctgcg
     1861 acgagcgcat cggtcgcatc tgcatctggt tcggcggcga ggacgaggag accttccccg
     1921 ccgagctccc ggacgtcgcg ttggcccgga cgaagctgat cggggcgctc gaggacgcct
     1981 tcgagcggac cccgcaccgc tgggtgatcg gacagctcgt ctactatctc gtggagagcc
     2041 ggaacgtgga cgaggcggaa agggtcgcgt cccggtgcgg gatcccggag acgtggtggt
     2101 gctccgcgct gctgggctac gtacatcaca tgcgcaggga gtacatcgag gcggaggccg
     2161 ccttccgcga ggcgctggcc tccatgcccg tgcccctgcg cgacgagtgg atgacccgct
     2221 acgtcctcac aggcgatgtc ctcgaggagc tggaggaggc ctcgccgacg gagagggacc
     2281 gga
//
LOCUS       DASQQQ010000026         4034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_112712, whole genome shotgun sequence.
ACCESSION   DASQQQ010000026 DASQQQ010000000
VERSION     DASQQQ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112712"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..1413)
                     /gene="coaBC"
                     /locus_tag="VE646_01450"
     CDS             complement(199..1413)
                     /gene="coaBC"
                     /locus_tag="VE646_01450"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008740397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HZD78690.1"
                     /translation="MAARQAPALAGRRVLLGVTGGIAAYKACTLTRLLTAAGAEVQVV
                     MTAAATRFVGPDTFSALSERPAYTDLWQAPGEVLHVRLAHEAHVAVVAPVTANVLAKL
                     ATGIADDLLTSTLLEASCPLVLAPAMHSGMWRHPTTRSNVEALAARGAHLVGPVSGPL
                     AAGDEGVGRMAEPEDIAAAVGSAVARGRDLAGRRIVVSAGPTWEPIDPVRFIGNRSSG
                     TMGFALAREALGRGAHVTLVVGPGTVPPPEGPVLVPVTTAEEMREAVLTAAGRADAVI
                     MAAAVADFRPEHTAEGKLKKEGGPPELRLVPTPDILRDLARDRGGAILVGFAAETSDV
                     MEAGRAKLRDKGVDLLVVNEVGREGTGFGSITDEAAILSGREGEETPLRTWTKAALAA
                     AICDRLAARWAS"
     gene            complement(1461..1709)
                     /gene="rpoZ"
                     /locus_tag="VE646_01455"
     CDS             complement(1461..1709)
                     /gene="rpoZ"
                     /locus_tag="VE646_01455"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HZD78691.1"
                     /translation="MIEPKIDDLLAHVDSKYTLVILAARRAREINNYYSQLGEGRGEF
                     VPPLVETGGLKNKPLSIALEEISEGKILAERPEGIPGA"
     gene            complement(1706..2299)
                     /gene="gmk"
                     /locus_tag="VE646_01460"
     CDS             complement(1706..2299)
                     /gene="gmk"
                     /locus_tag="VE646_01460"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HZD78692.1"
                     /translation="MRTRRGRLFVVSGPSGVGKGSVVRQLLERDPEGLGYSVSYSTRE
                     ARLGEVPGRDYVFVPPHEFERMTVAGRFLEWAEVFGHRYGTAAEPVERMLAEGRDVVL
                     EIDVQGARQVHERVPDAVLIFLVPPSLAELERRLRSRGTEPEDKLARRLEKAAFEMDQ
                     SSWFDHVVVNDDLEEATAQVEAIIQTSRNPTQGSTHP"
     gene            complement(2306..2620)
                     /gene="mihF"
                     /locus_tag="VE646_01465"
     CDS             complement(2306..2620)
                     /gene="mihF"
                     /locus_tag="VE646_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HZD78693.1"
                     /translation="MPLPVLTEEQRKQALEKAAEARRKRAELKGELKSGKLTLKDVLS
                     RQDDDTVGKMKVSTVLESLPGVGKVRARKIMEKLDISASRRVRGLGAKQRESLLGEFG
                     KK"
     gene            complement(2758..3441)
                     /gene="pyrF"
                     /locus_tag="VE646_01470"
     CDS             complement(2758..3441)
                     /gene="pyrF"
                     /locus_tag="VE646_01470"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HZD78694.1"
                     /translation="MIPRPANPLIAALDVSDLDRAEELARALSGGVGVLKVGLELFAA
                     HGPRAVERIRAHGPVFLDLKLHDIPTTVERAARAVGRLGVSMLTVHALGGQAMVAAAV
                     RGVTEGSQDAGHAVPTVLAVTVLSSLSGEELASPASLAFEARAAGAGGTVVSGQDVAL
                     VREVTGEDFCLVVPGIRPAGSNGHDQVRILTPEEAVDAGADYLVVGRSITASADPAGA
                     ARSILSTIR"
     gene            complement(3438..>4034)
                     /locus_tag="VE646_01475"
     CDS             complement(3438..>4034)
                     /locus_tag="VE646_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HZD78695.1"
                     /translation="ALELYLGTRDEGHGGTPLYDRRELLVDVVGAVSRLSSLPVFAKL
                     SPVVPNLEETARACVRAGAHGLTLIDALPGMVVDAARLRPRLGAVIGGLSGPAIRPMA
                     LAAVYRVSQALPAVPIMGVGGIATGEDAVEFLLAGAWAVQVGTAMLVDPAVPEEICRG
                     IMGYLEDKGLGSPGELRGRLRGGAAPEPQPADGEIEAR"
BASE COUNT          560 a         1537 c         1386 g          551 t
ORIGIN      
        1 gtcccaccgg gtcggcgcgg aggatctcgt ccaggacggc gtccgagatg cggtcggcga
       61 gcttgtcggg atgcccctcg gtgaccgact cggacgagaa gagatagccg tgacccatga
      121 ccgcgcgagt atatccgtac ccggagacct gaccgccaac cgccggtcga ccgcgcctcc
      181 gctgcaagcg ctccaggatc aggaggccca gcgagcggcc agccggtcgc agatcgcggc
      241 ggccagggcg gccttggtcc aggtgcggag cggcgtctcc tcgccctcgc gtcctgacag
      301 gatggccgcc tcgtccgtga tcgagccgaa cccggtgccc tcccggccca cctcgttcac
      361 caccagcagg tccaccccct tgtcccggag cttggcgcgt ccggcctcca tcacatcgga
      421 ggtctcggcg gcgaacccca ccaggatcgc gccgccccga tcccgggcga ggtcgcggag
      481 gatgtccggg gtcgggacca gccgcagctc gggaggtccg ccctccttct tcagcttgcc
      541 ctcggccgtg tgctcggggc ggaagtcggc caccgcggcg gccatgatca ccgcgtcggc
      601 ccgaccggcc gccgtcagga ccgcctcgcg catctcctcg gccgtggtga ccggcaccag
      661 gaccggcccc tcggggggcg gcacggtgcc cggcccgacc accagggtca cgtgggcacc
      721 ccgtcccaag gcctcgcggg ccagggcgaa gcccatcgtg ccggaggaac ggttgccgat
      781 gaaccgcacg ggatcgatcg gctcccaggt gggcccggcg gagaccacga tccgccgccc
      841 ggccaggtcc cgccctcgag ccaccgccga tccgaccgct gccgcgatgt cctccggctc
      901 ggccatccgg ccgaccccct catcgccggc cgccagcgga ccgctcaccg gccccaccag
      961 gtgcgccccg cgggcggcca gggcctccac gttcgagcgg gtggtggggt gccgccacat
     1021 ccccgagtgc atggcgggtg ccagcacgag cgggcaggag gcctcgagca gcgtggaggt
     1081 gagcaggtcg tcggcgatcc cggtcgcgag cttggccagg acgttcgccg tgaccggcgc
     1141 gaccaccgcc acgtgggcct cgtgcgccag ccggacgtgc aacacctcgc cgggagcctg
     1201 ccacagatcg gtgtaggcgg gacgctccga gagcgcggag aaggtgtcgg ggccgacgaa
     1261 ccgggtggcg gccgcggtca tcaccacctg gacctcggcc ccggcggcgg tcagcaggcg
     1321 cgtgagcgtg caggccttgt atgcggcgat gccgccggtg acgccgagga gcacccggcg
     1381 tcccgccagc gcgggggcct gcctggctgc cacctcaccc cccgcgatcg gctgggctcg
     1441 gggccgagcc cgtcggcggg ctaggcgccg gggatcccct cgggacgctc ggccaggatc
     1501 ttgccctcgg agatctcctc cagggcgatc gacaggggct tgttcttcag cccgccggtc
     1561 tcgaccagcg gtggcacgaa ctcgccacgc ccctcgccga gctggctgta gtagttgttg
     1621 atctccctgg cccgtcgggc cgccaggatc accagcgtgt acttggagtc cacgtgcgcg
     1681 agcaggtcgt cgatcttcgg ttcgatcatg ggtgcgtaga tccttgggtc gggttccggg
     1741 acgtctggat tatagcttcc acttgcgcgg tcgcctcctc caggtcgtcg ttcaccacca
     1801 cgtggtcgaa ccaggaggac tgatccatct cgaacgcggc cttctccagg cggcgagcca
     1861 gcttgtcctc cggctccgtg ccccgagagc gcagtcggcg ttcgagctcg gccagcgagg
     1921 gcgggaccag gaagatcagg accgcgtcgg gcacccgctc gtgcacctgc cgggcccctt
     1981 ggacgtcgat ctccagcacg acgtcgcgcc cctcggccag catccgctcc accggctccg
     2041 cggcggtccc gtagcggtgg ccgaagacct ccgcccattc caggaaccgc cccgccaccg
     2101 tcatccgttc gaactcgtgc ggcggcacga acacgtagtc acgacccggg acctcgccca
     2161 gtcgcgcctc gcgggtcgag taggagacgg aatagccgag cccctccgga tcgcgctcga
     2221 ggagctgtct tacgacgctc cccttgccca ctccggaggg gccggagacg acgaagagcc
     2281 ttccgcggcg cgtgcgcagc tcgcgttact tcttgccgaa ctcgccgagc agggactcgc
     2341 gctgcttggc gccgagacca cgcacccgac ggctggcgga gatgtccagc ttctccatga
     2401 tcttccttgc ccggaccttc cccacgccgg gcagggactc gaggacggtg gacaccttca
     2461 tcttcccgac ggtgtcgtcg tcctgcctgg acagcacgtc cttcagcgtg agcttcccgg
     2521 acttcagctc gcccttgagc tcggcccgct tgcggcgcgc ctccgcggcc ttctccaggg
     2581 cctgcttccg ctgttcctcc gtcaggacgg gtaggggcat gtcgcgctcc ttttctggtt
     2641 ggggtcagag ccacgcgagt atacgcacgg gtcccgagga ccagcaacgc gacgcgcggt
     2701 gtcacctgcg gtttcgctcg gtgccgtcaa cctcgccctg agggtgaggc ccgaggatca
     2761 tcggatcgtc gagaggatcg accgggccgc gccggccgga tcggccgacg cggtgatgga
     2821 ccggccaacc accaagtagt cggcgcccgc gtcgacggcc tcctcgggcg tgaggatcct
     2881 gacctgatcg tgaccgttcg agccggccgg acggatgccc gggaccacca agcagaagtc
     2941 ctcgccggtc acctcgcgca ccagggccac gtcctgtccc gagaccacgg tgccgcccgc
     3001 cccggccgca cgcgcctcga aggccaggga cgcaggggag gcgagctcct cccccgacag
     3061 gctggacagc acggtcacgg ccagcaccgt cggcacggcg tgaccggcat cctgcgagcc
     3121 ctcggtgacg ccccgcacgg ccgcggccac catggcctgc cccccgaggg catgcaccgt
     3181 gagcatcgag acgcccagcc ggcccaccgc gcgggcggcg cgctccacgg tggtggggat
     3241 gtcgtgcagc ttcaggtcca ggaacacggg gccgtgggcg cggatccgct ccacggcccg
     3301 agggccgtgc gccgcgaaca gctcgagccc cacctttagg acgcccaccc cgcccgagag
     3361 cgcccgggcg agctcctcgg cccggtccag gtccgagacg tccagagccg cgatcagcgg
     3421 gttcgccggc cgagggatca ccttgcttcg atctctccgt cggcgggctg gggctccggc
     3481 gcggcgcccc cccggagccg gccgcgcagc tccccgggcg aacccaagcc cttgtcctcc
     3541 aggtagccca tgatgccccg gcagatctcc tcggggacgg cgggatcgac gagcatcgcg
     3601 gtgcccacct gcaccgccca cgcgccggcc agcaggaact cgaccgcgtc ctccccggtg
     3661 gcgatgcccc cgacccccat gatcggcacg gccgggaggg cctgcgatac gcggtacacg
     3721 gccgccagcg ccatggggcg gatcgccggt ccggacaggc cgccgatcac cgcccccagc
     3781 ctgggtcgca ggcgcgccgc gtcgaccacc atgcccggca gggcgtcgat cagggtgagc
     3841 ccgtgcgcac cggcccggac gcacgcccgg gccgtctcct ccaggttcgg caccacgggg
     3901 gacagcttgg cgaacaccgg gagcgaggac aggcgggaga ccgcccccac cacgtcgacc
     3961 agcagctcgc ggcggtcgta gaggggcgtg cctccgtgcc cctcgtcccg ggtcccgagg
     4021 tacagctcca gggc
//
LOCUS       DASQQQ010000027         3837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_221764, whole genome shotgun sequence.
ACCESSION   DASQQQ010000027 DASQQQ010000000
VERSION     DASQQQ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221764"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1765)
                     /gene="nuoL"
                     /locus_tag="VE646_01480"
     CDS             complement(<1..1765)
                     /gene="nuoL"
                     /locus_tag="VE646_01480"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HZD78696.1"
                     /translation="MPVMGLIPLLPLLGAATLLLTGKRWRGTSSGWLASTLVLGSFVV
                     GWIVLFALLGDPASARVSVARFWEWIPVGSLHVPIELRADPLSMVMVLTVSGVATIIH
                     LYAIDYMRDDPRYGRFFAYMNLFVFFMLMLVLADNFLLLYLGWEGVGLCSYLLIGFWF
                     ERPSAAFAAKKAFIVTRIGDSAFLIGIILIWTHFHTLAFDPIFRGAGGLANGTATVMA
                     LLLLAGAVGKSAQLPLHVWLPDAMEGPTPVSALIHAATMVTAGVYLVVRAHAIFEASS
                     VAPTVVAFVGLITAIYSGLSAIGQDDIKRMLAYSTISQLGFMFFGAGMGAYAAAIFLL
                     VAHAFFKALLFLSAGSVMHGLPNDETDMMRMGALRKVMPVTAYTWVVGWLAMAGIPPL
                     SGFFAKDQVVAAAQTSGRNALWIAVLFAAVLTAVYETRATFIAFFGDARYAGHPHDPP
                     PRMRLGMILLAIGAAAGGVLGLTESSGVMARFLDPVVGVPHQVAGALSTAALSAISVA
                     VAVVGIGIAWLVYLSGRVDWKALRERYPGQKRTLLHGFFVNDLYNALLVANAQLASAF
                     VASVVDLRIVDGIVNGVGWVVR"
     gene            complement(1773..2078)
                     /gene="nuoK"
                     /locus_tag="VE646_01485"
     CDS             complement(1773..2078)
                     /gene="nuoK"
                     /locus_tag="VE646_01485"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HZD78697.1"
                     /translation="MSTPIGYFLAVSALLFSVGTVGVLVRKNALVIFMSIELQINAVN
                     LALVAFSRRLGNLTGQTLAFFVMVVAAAEVVVGLAIIVSLYRKRRSADVDDADLLRW"
     gene            complement(2075..2674)
                     /locus_tag="VE646_01490"
                     /pseudo
     CDS             complement(2075..2674)
                     /locus_tag="VE646_01490"
                     /inference="COORDINATES: protein motif:HMM:NF005165.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
     gene            complement(2694..3164)
                     /gene="nuoI"
                     /locus_tag="VE646_01495"
     CDS             complement(2694..3164)
                     /gene="nuoI"
                     /locus_tag="VE646_01495"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HZD78698.1"
                     /translation="MRTGAFGAIAKGMGTVFRQIFRPDVTERYPRQVNPPPPRSHIGR
                     HRLNRHENGLEKCIGCELCAYACPADAIWVEGADNDPEAPTSPGERYAKDYQINYLRC
                     IMCGLCVEACPTRALTLTSAYEMSYATREDAVLTKEQLLQPPLPLGTAPGTEEG"
     gene            complement(3230..>3837)
                     /locus_tag="VE646_01500"
     CDS             complement(3230..>3837)
                     /locus_tag="VE646_01500"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HZD78699.1"
                     /translation="YSGTLTMSGIVASQDRIWNVIPQLPAFLIYLVAGLAETNRPPFD
                     LPEAESELVAGYHTEYSGIKFALFYLGEYLNVVTVASIATTLFLGGWRGPHPNVVPWL
                     WGVLWFLIKVVAIVYVYVWIRATLPRMRYDRLMDFGWKVLIPVGLVWVLATGAVVILP
                     DLYGRRAVLLGAAAVVGVVLLLTLLWPLFAPRRGMPEEVRP"
BASE COUNT          705 a         1359 c         1300 g          473 t
ORIGIN      
        1 tgcggaccac ccaacccacc ccgttcacga tcccgtcgac gatgcgcagg tccaccacgg
       61 aggccacgaa ggccgaggcc agctgggcgt tggccaccaa cagggcgttg tagagatcgt
      121 tcacgaagaa gccgtgcagg agcgtgcgct tctgccccgg gtagcgctcg cgcagcgcct
      181 tccagtccac ccgacccgag agatagacga gccaggcgat cccgatcccc accacggcca
      241 ccgccaccga gatcgcggac agcgccgccg tggacaacgc gccggcgacc tgatggggca
      301 caccgaccac cgggtccagg aaccgcgcca tgacgccgct gctttcggtc aggccgagga
      361 ccccgccggc cgcggccccg atggccagca ggatcatccc caggcgcatc cggggcgggg
      421 ggtcgtgggg atgacccgcg taccgggcat ccccgaagaa cgcgatgaac gtggcccgcg
      481 tctcgtagac cgcggtcagg acggcggcga acagcaccgc gatccacagg gcgttcctcc
      541 cgctggtctg ggccgccgcc accacctgat ccttggcgaa gaacccggac agcgggggga
      601 tgccggccat ggccagccac cccaccaccc aggtgtacgc ggtgacgggc atgaccttgc
      661 gcaaggcccc catgcgcatc atgtcggtct cgtcgttcgg caggccgtgc atcacgctgc
      721 cggcggacag gaacagcagg gccttgaaga aggcgtgggc caccagcagg aagatcgcgg
      781 ccgcgtaggc ccccatgccg gcaccgaaga acatgaaccc gagctggctg atcgtggagt
      841 acgccagcat ccgcttgatg tcgtcctggc cgatggcgga cagccccgag tagatcgcgg
      901 tgatcaggcc cacgaaggca acgacggtgg gcgcgaccga ggacgcctcg aagatggcgt
      961 gggcgcgtac caccaggtac acgccggccg tcaccatggt ggccgcgtgg atcagcgccg
     1021 agaccggcgt ggggccctcc atcgcgtccg gcagccacac gtgcaggggg agctgggccg
     1081 atttccccac cgcgccggcg agcagcagca aggccatgac cgtggccgtc ccgttggcca
     1141 ggccgccggc gccgcggaag atcgggtcga aggcgagcgt gtggaagtgg gtccagatca
     1201 ggatgatccc gatcaggaag gcggagtccc cgatccgggt gacgatgaag gccttcttcg
     1261 ccgcgaaggc cgccgagggg cgctcgaacc agaagccgat cagcaggtag ctgcagagcc
     1321 ccacgccctc ccagcccagg tacagcagga ggaagttgtc cgccaggacc agcatcagca
     1381 tgaagaagac gaacaggttc atgtaggcga agaaccggcc gtaccggggg tcgtcgcgca
     1441 tgtagtcgat cgcgtacagg tggatgatcg tggccacccc cgagacggtg agcaccatga
     1501 ccatcgagag cgggtcggcg cggagctcga tcggaacgtg cagggagccg accgggatcc
     1561 actcccagaa tcgcgccacc gacacgcggg ccgatgccgg gtcccccagc agggcgaaca
     1621 ggacgatcca ccccaccacg aacgacccga gcacgagcgt cgatgccagc caccccgagg
     1681 aggtgcccct ccaccgcttg ccggtcagca agagcgtggc cgccccgagg agcggcagca
     1741 gggggatgag ccccatcacc ggcatgtcgc cctcaccatc gcagcaggtc cgcgtcgtcc
     1801 acgtcggcgc tgcgccgctt ccggtacagg ctcacgatga tcgccagccc caccacgacc
     1861 tcggccgccg ccaccaccat cacgaagaag gccagggtct gtccggtgag gttgcccagc
     1921 cgccgagaga acgccaccag ggccaggttc acggcgttga tctgcagctc gatcgacatg
     1981 aagatcacca gggcgttctt gcgcaccagc acgcccaccg tccccaccga gaacagcagg
     2041 gcggacaccg ccaggaagta cccgatcggc gtgctcacga aggcgcctcc tcgagctgct
     2101 cttcctcggg caccccggtc gcctcgtgcc cctcggggac cggctgccgg gtccggcgtc
     2161 ccgcgtgcag catcgctccg atcgccgcga tgatcagcag caccgaggtc acctcgaacg
     2221 gcaggaagta cgagcggaac aggaccccgg ccagggcctg cgtgttgccg ccctggttca
     2281 ccacccggtc gaagtccgcc ggaccggtcg ccgcgaaccc gaccccggcg tggatcgcgg
     2341 tgacgagctc ggcggcgagg cccaagccca gcacgatggc cagcggccgc tgaccgcgca
     2401 gggtctcgat caggctctcc tcgcgatcca cgccgagcag catgatcacg aacaggaaca
     2461 gcaccatgat ggccccggcg tacacgatga tctgcaccac gaacaggaac ggggcgtcca
     2521 gcagcaggaa gaacacggcc agggtgaaga agttcacgat gagaaacagc gccgcgtgga
     2581 tcgcgttgcg ctggaagagc atcgcgatgg ccgacccgac agagatgggg gcgaacaccc
     2641 agaacaccac ctgatcggcc gtcacccgcg cccccgctgc acgccgggca cgatcagcct
     2701 tcctccgtcc cgggcgcggt accgagcggc aaaggcggct gcagcagctg ctccttggtg
     2761 aggacggcgt cctcgcgcgt ggcgtaggac atctcgtacg cgctggtgag ggtgagggca
     2821 cgggtggggc aggcctccac gcacagaccg cacatgatgc agcgcaggta gttgatctgg
     2881 tagtccttcg cgtaccgctc cccgggagag gtcggggcct ccgggtcgtt gtcggccccc
     2941 tccacccaga tcgcgtcggc cgggcaggcg tacgcgcaca gctcgcagcc gatgcatttc
     3001 tccaagccgt tctcgtgccg gttcaggcgg tgccggccga tgtgggagcg gggcgggggc
     3061 gggttgacct gcctcgggta tcgctcggtg acgtcggggc ggaagatctg ccggaagacc
     3121 gtgcccatgc ccttcgcgat cgcgccgaac gcgccggtgc gcacggcccc cccaggctcg
     3181 ttcgggccct cggtcggggc cgcgcggtcc tgtcggggtc ccggaccact cacggccgga
     3241 cctcctcggg catcccgcgc cgcggtgcga acagcggcca cagcaacgtg aggaggagca
     3301 ccacgcccac cacggcggcc gcgcccagca gcaccgcccg gcggccgtac aggtcgggaa
     3361 ggatcaccac ggcgccggtg gccagcaccc agaccaggcc gaccgggatc agcaccttcc
     3421 acccgaagtc catcagccgg tcgtagcgca tccggggcag ggtggcccgg atccagacgt
     3481 agacgtagac gatggccacg accttgatca ggaaccacag cacgccccac agccacggga
     3541 ccacgttcgg gtgggggccg cgccagccgc ccaggaacag cgtcgtggcg atggaggcca
     3601 ccgtcaccac gttcaggtac tcccccaggt agaacagggc gaacttgatc cccgagtact
     3661 cggtgtggta cccggccacc agctcggact ccgcctccgg caggtcgaac ggcgggcggt
     3721 tggtctcggc caggccggcc accaggtaga tcaggaacgc gggcagctgg gggatcacgt
     3781 tccagatccg gtcctgggag gccacgatcc cgctcatcgt gagcgtcccc gagtaca
//
LOCUS       DASQQQ010000028        12412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_57807, whole genome shotgun sequence.
ACCESSION   DASQQQ010000028 DASQQQ010000000
VERSION     DASQQQ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12412
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57807"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..547
                     /locus_tag="VE646_01505"
     CDS             38..547
                     /locus_tag="VE646_01505"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004930547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZD78700.1"
                     /translation="MTEWDLTTKPVSFSQITLVQLMEITDANIAGIVHGGVVMKLVDT
                     AAGLAAIRHCGGLAVTVSMDEMSFLAPVRIGDTLTVRASVNDVGRTSLEVGVRVESEN
                     VVTGERTHTGSAYLVFVALDDQGKPRPIPPVIAETPTQQRRQREAKMRREARLAHKAA
                     IREHRSREG"
     gene            598..3330
                     /gene="acnA"
                     /locus_tag="VE646_01510"
     CDS             598..3330
                     /gene="acnA"
                     /locus_tag="VE646_01510"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HZD78701.1"
                     /translation="MTRDLAGARTRLDTGSGPVTIHRLGWLAEQGVGEVGRLPHTVKI
                     LLENLLRRAGTRDVGEEDVRALARWPEPVAHANLAYMPSRVLMQDFTGVPAVVDLAAM
                     RSAMARAGGDPGKVNPLVDVDLIIDHSVQVDLFRSPEAYQANIEWEYRRNGERYALLR
                     WAQQAFDGMRVVPPGAGICHQVNLEHLGRVVTTKDGAAFPDTLVGTDSHTTMVNGLGV
                     LGWGVGGIEAEAAVLGQPMFLPTPIVVGVRVTGGLPAGTTATDLVLTLTQMLRARGVV
                     GRFVEFFGPGCSSLELADRATLSNMCPEYGATAAFWPVDEETLRYLRFTGREDRVDLV
                     ERYTKEQGLFRTDEDPEPIFSEVVDLDLSAIEPSVAGPKRPQDRVPLPGVWDSFVEAF
                     RDRMGPDPEPTALDRLVAEGGRPESPVEEGGAVDPSAPAVAVDGQVRHGSVVIAAITS
                     CTNTSNPSVMLAAGLLARKAVEAGLQSRPWVKTSLAPGSRVVTEYLDAAGLQPYLDKL
                     GFSLVGFGCTTCIGNSGPLPEEVERAVRDRDLAVCAVLSGNRNFEGRIHPLVRASYLA
                     SPPLVVAYALAGHVGTDLTAEPLGTDADGSPVYLRDLWPSAEEVREAIAASVSPEQFK
                     REYGRIFQGDEHWNGLPSPTGPMYEWDPDSTYVREPPFFQDLGPEPEEVHDLEGARVL
                     VKVGDSITTDHISPAGSIKPDSPAGEYLIDHGVQQRDFNSYGSRRGNHEVMMRGTFAN
                     VRLRNELAPGTEGPWTTHLPSGDRLSIFDAAERYRAEGTPLLVVAGKEYGSGSSRDWA
                     AKGPSLLGVRAVIAESYERIHRSNLVGMGVLPLQFTDGRTAETLGLTGREVYAVTGLR
                     AGLRPRQTVTVTARGQDGEEVSFEALVRIDEEAEVDTFRHGGILQMVLRQLLRA"
     gene            3417..4544
                     /locus_tag="VE646_01515"
     CDS             3417..4544
                     /locus_tag="VE646_01515"
                     /inference="COORDINATES: protein motif:HMM:NF017072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78702.1"
                     /translation="MELSVGLPRMHKEAGERRDFLPGFVAFLKRTGFGDIVIEHGYGQ
                     GVGFEEKTYLKRSERARFASLEECLAQDVLAVIRCPDEEAIRRMRRGSILLTMIHYPT
                     RPERVELLTELGIHGVSLDGITDDAGRRLVENLQAVGWNGVHAAFHEVASSHPRFAHP
                     SRRPIRVTCLGAGAVAGYAIRAATRYGDPKLREELVARHVPGVEVTVVDFDLTWHEYY
                     MLTRLEQTDLLIDATQRTDPSRVIVPNDWVAALPQDAVILDLSVDPYDFSVDPPKVKG
                     IEGVPEGTLDQYVFHPEDPAYERMDPRIRRGHRRVALSCYSWPGIQPRECMQVYGKQL
                     EPVMRVIARKPVQTWDAHHGTHYERAVARAEVNRWRVENVR"
     gene            4520..5653
                     /locus_tag="VE646_01520"
     CDS             4520..5653
                     /locus_tag="VE646_01520"
                     /inference="COORDINATES: protein motif:HMM:NF017072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HZD78703.1"
                     /translation="MAGRERALTFGLPRMHKEAGERRDFLPALVGKLTDRGAEVLVER
                     GIGSGMGFRDRDYAALSRLVRLVTNREAFGADVVLTLRCPDESEFTKLRSGALLFSML
                     HFSTRPKRVRRLHDLGLEAISMDSITDDHGRRLVEDLKAVAWNGLEAAFEALASGWPD
                     LERPDRGPVQVTIMGAGGIGKHAVEAATKFGDLARNARYAEADLSGVQVVTVGRNVTG
                     REQHLRALLRRTDVLVDATHRDEPWRPLVPNAWIADLPEHAVICDLVVDPYLPEGDPP
                     TVRGIEGIPQGNLDGWVFVRDDPVWDLAPPSVSTEHRRTVVSCYSWPGVHAVPCMEQY
                     GTQLAPLLEELLRAGGMDGLRPEGAYHERALYRASLRAWLAVG"
     gene            complement(5698..6735)
                     /locus_tag="VE646_01525"
     CDS             complement(5698..6735)
                     /locus_tag="VE646_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HZD78704.1"
                     /translation="MSIDLRTTYLGLELANPIVPSASTLSSRTDNLKRMQDAGAAAVV
                     MQSLFEEQIEHDELEIFRVLETGAESFAEALTYFPELEDYNTGPDEYLRHLEMTKREL
                     QIPVIGSLNGASPGGWVRYAKLIEQAGADALELNVYFIAADPDETGEQVERRYLDLVS
                     EVRSEVGIPLAVKIGPSFSSPANMARQLDRAGADGLVLFNRFYQPDIDLETLEVDPTL
                     HLSTSDECLLPLRWIAILRGRLGCSLAATTGVHTWEDSVKLLLAGADVTMMASALFRN
                     GPEHVASVLAGLERWLEEREYVSVEQMKGSVSQANVADPTAFARANYMQMIVSYTSPY
                     DWRMGTAEPRA"
     gene            complement(6732..10358)
                     /gene="nifJ"
                     /locus_tag="VE646_01530"
     CDS             complement(6732..10358)
                     /gene="nifJ"
                     /locus_tag="VE646_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015182979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HZD78705.1"
                     /translation="MTGRKTLVTVDGNEATARIAHKLSEVIAIYPITPSSPMAEHADA
                     WSAAGRTNLWGVAPQVIEMQSEGGAAGALHGALQGGALGTTFTASQGLLLMIPNMFKI
                     AGELTPAVIHVAARTVATHALSIFGDHSDVMAVRPTGWGMLASSSVQSAQDMAAVAHV
                     STLEARVPFLHFFDGFRTSHEVNVIEAVEDDVLRELMDDHLVRAHRERALSPDHPVLR
                     GSAQNPDVFFQAREAANPFYQVVPDIVQYTMDRFGELTGRHYHLFDYSGAPDAERVVV
                     LMGSGVGAVRETVERLVEDGERVGVLEVHLYRPFSAAALVSSLPPTVTSVAVLDRTKE
                     PGALGEPLYQDVLTAFAEEGAAGRLRAVPRVIGGRYGLSSKEFNPPMAKAVFDELAKD
                     EAKRHFTIGIHDDVSHTSLSWDPTFRTEPKDVVRALFYGLGADGTVSANKSSVKIIGE
                     GTDLYAQGYFVYDSKKSGSMTTSHLRFGPRPIEGAYLIRQAGFVACHQFNFLERTDVL
                     AAAEPGATFLLNSPYGPDEVWGHLPLETQRAIVDKGLRFYVIDAQTIADEVGLGTRIN
                     TILQTCFFALAGILDQPEAIARIKQAIEKSYGKRGQSVLDMNYAAVDRALEGLHEVRI
                     PVETSGDLHRLPPVPDDAPDFVKRVTAMMIAGQGDLLPVSALPPDGTFPTGTARWEKR
                     SIAHEIPIWDPSICIDCAKCALVCPHAAILMKVYDPSDAEGAPDTFQHKEWRDREHPG
                     RWMTIQVAPDDCTGCGVCVDICPAKSKEAVKHKSIDMLPKLEHLEAERENFEFFQTIP
                     RPDRREVKVASIKGSQMLEPLFEFSGACAGCGETPYLRLLSQMFGDRIMIANATGCSS
                     IYGGNLPTTPWSKDVNGRGPAWSNSLFEDNAEFGLGMRLALDQQLAQARELLPKLAPE
                     LGQDLVEAILAADPREETDEGGLAEQRERVAELKRRLEGLESPEARDLLAVADVLVRK
                     NVWIVGGDGWAYDIGYGGLDHVLASGRDVNVLVLDTQVYSNTGGQASKATPRGAVAKF
                     AAGGKAVGRKDLGMIAAEYGNVYVAQVAIGADNAQTVKAFAEAESWHGPSLIIAYSTC
                     IEHGIDMSTSLTHQAEIVKSAFWPLYRYDPRKATDSERALHLDSKAPSLKLKDFQAKE
                     ARFAMLARSRPEEFGELQELAQEDAEERWRLYEQLAGVQRHAPGELNGHEKRGRDGGK
                     EGSA"
     gene            complement(10464..11039)
                     /locus_tag="VE646_01535"
     CDS             complement(10464..11039)
                     /locus_tag="VE646_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZD78706.1"
                     /translation="MASEAVHLEEPRFAKWLFDSTAASWIWLVVRVYFGYEWANAGWE
                     KIQGGWLGSSAGIAGFSAGALHGAAQGPHSAVNYGWYASFLHWVGGDGAGIMTRVIPL
                     GEMAIGIALIVGLFTGVSAFFAGMLSMSFGLAGVAGVNPVFFLAEVLLVLAWRNAGYI
                     GLDRVVLPALGTPWEPGRLFHRVKEPTRQLA"
     gene            complement(11191..11868)
                     /locus_tag="VE646_01540"
     CDS             complement(11191..11868)
                     /locus_tag="VE646_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016579326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD78707.1"
                     /translation="MTKGTKEPPLRVLLVDDHEVVRSGVKALLQATEDIIVTAEAGTI
                     REAIDEADRSRPDVVVMDVRLADGSGIEATREIRARHPKTSVVMLTSFADDEALFASI
                     MAGASGYVLKQVKGGELVRAIRTVGKGESLLDPAVTNAVLERLRKGKHLLKDEKLARL
                     SPQEERILSLVADGKTNKEIGEDLRLAEKTVKNYVSSILSKLEVARRAEAAAYLARHT
                     TTPGTQS"
     gene            complement(11928..>12412)
                     /locus_tag="VE646_01545"
     CDS             complement(11928..>12412)
                     /locus_tag="VE646_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78708.1"
                     /translation="MFIRYYLDLAMRFAEVEEKLLHDPASWLPGIAEGAEDRGERLLV
                     DVGFPASDRRVAKSVEIELGNPYRSPGKVLLPLTWRATRTEGLFPILEADMEIAGLGA
                     DRTQLSLSGRYRPPLGALGRVLDRALLHRVAEATVKDFLDGVGQMLRNGLPAATGTGA
                     "
BASE COUNT         1775 a         4331 c         4446 g         1860 t
ORIGIN      
        1 atccgccgcc atgctaccgg tccggtacag tgcggccatg accgagtggg acctcaccac
       61 gaagcccgtg tcgttctccc agatcacgct ggtgcagctg atggagatca ccgacgcgaa
      121 catcgccggg atcgtccacg gcggggtggt gatgaagctg gtggacacgg cggcgggcct
      181 ggccgccatc cggcactgcg gcgggctggc ggtcacggtc tcgatggacg agatgtcgtt
      241 cctggcgccg gtccgcatcg gggacacgct cacggttcgg gcctcggtga acgacgtcgg
      301 gcgcacgtcc ctggaggtcg gggtgcgcgt tgagtcggag aacgtggtca cgggggagcg
      361 gacgcacacc ggtagcgcgt acctcgtgtt cgtggcgctg gacgatcagg gcaagcccag
      421 gcccatcccg ccggtgatcg cggagacgcc cacgcagcag cggcggcagc gcgaagccaa
      481 gatgcgccgg gaggcgcgcc tggcccacaa ggccgcgatc agggagcatc ggagccggga
      541 aggatgaacc gggacggctc cacgttcccg gggcccgacg atcgaggaag gaccgacatg
      601 acgagggatc tggcaggagc ccggacccgg ctggacaccg ggtcgggacc ggtgacgatc
      661 caccggctgg gctggctggc cgagcagggg gtcggcgagg tggggcggct gccccacacg
      721 gtgaagatcc tgctggagaa cctgctccgg cgcgccggga cccgtgacgt gggcgaggag
      781 gacgtgcgcg cgctcgctcg ctggcccgag ccggtggccc acgccaacct cgcctacatg
      841 ccctcgcgcg tgctgatgca ggacttcacc ggtgtcccgg cggtggtgga cctggccgcg
      901 atgcgctcgg ccatggcccg ggcgggagga gatccgggca aggtgaaccc gctggtcgac
      961 gtggacctga tcatcgacca ctcggtccag gtggacctgt tccggagccc cgaggcctac
     1021 caggccaaca tcgagtggga gtaccggcgc aacggcgagc gctacgccct gctccgctgg
     1081 gcccagcagg ccttcgacgg gatgcgggtg gtcccgccgg gggccgggat ctgccaccag
     1141 gtgaacctcg agcacctggg gcgggtggtg accacgaagg acggcgctgc cttccccgac
     1201 acgctggtcg gcaccgactc ccacaccacg atggtgaacg gcctgggggt cctgggctgg
     1261 ggggtcggag ggatcgaggc ggaggcggcg gtgctcgggc agcccatgtt cctgcccacg
     1321 cccatcgtgg tcggggtccg ggtgaccggg ggcctgcccg cgggcaccac cgccacggac
     1381 ctggtgctca cgctcaccca gatgctgcga gcccgtggcg tggtgggccg gttcgtcgag
     1441 ttcttcggcc ccggttgctc ctcgctggag ctggccgacc gggccaccct gtcgaacatg
     1501 tgccccgagt acggggcgac cgcggccttc tggccggtgg acgaagagac cttgcgctac
     1561 ctgcggttca ccggccgcga ggaccgggtg gacctggtcg agcggtacac gaaggagcag
     1621 gggctcttcc ggaccgacga ggatccggag cccatcttca gcgaggtggt ggacctggac
     1681 ctgtcggcga tcgagccctc ggtggccggg cccaaacgtc cccaggaccg ggtccccctg
     1741 ccgggggtct gggactcgtt cgtggaggcg ttccgggacc gcatgggacc cgacccggag
     1801 cccaccgcgc tcgaccgcct ggtggccgag gggggacggc ccgagtcacc ggtggaggag
     1861 gggggtgcgg tggacccctc ggccccggcc gtggccgtgg acgggcaggt gcggcacggg
     1921 tcggtggtga tcgccgccat caccagctgc acgaacacct ccaacccctc ggtgatgctg
     1981 gcggccggcc tgctggcgag gaaggcggtg gaggcggggc tgcagagccg gccctgggtg
     2041 aagacctcgc tggcgcccgg ctcccgggtg gtcaccgagt acctggacgc ggcgggcctg
     2101 cagccgtacc tggacaagct gggattctcg ctggtggggt tcgggtgcac cacctgcatc
     2161 gggaactccg gtcccctgcc cgaggaggtc gagcgggccg tgagggaccg ggacctggcg
     2221 gtgtgcgcgg tgctgtcggg gaaccgcaac ttcgagggtc ggatccatcc cctggtgcgg
     2281 gccagctacc tggcgtcccc cccgctggtc gtggcgtacg cgctggcggg tcacgtgggg
     2341 accgatctca ccgccgagcc gctgggcacg gatgccgacg gcagccccgt gtacctgcgt
     2401 gatctctggc cctcggcgga ggaggtgcgc gaggcgatcg cggcctcggt gtccccggag
     2461 cagttcaagc gcgagtacgg tcggatcttc cagggggacg agcactggaa cggcctgccg
     2521 tcgccgaccg ggccgatgta cgagtgggac cccgattcca cctacgtgcg tgagccgccg
     2581 ttcttccagg acctcgggcc cgagcccgag gaggtccacg acctcgaggg cgccagggtg
     2641 ctggtgaagg tgggggactc catcaccacc gaccacatct cgccggccgg ctcgatcaag
     2701 cccgactcgc cggcgggcga gtacctgatc gaccacgggg tccaacagcg ggacttcaac
     2761 agctacggat cgcggcgcgg gaaccacgag gtgatgatgc gcggcacgtt cgccaacgtg
     2821 cgcctgcgca acgagctggc tcccggcacg gagggtccct ggaccacgca cctgccgagc
     2881 ggagatcggc tgtcgatctt cgacgccgcg gagcgctacc gggccgaggg cacgccgctg
     2941 ctggtcgtgg ccggcaagga gtacggatca ggctcgtcgc gggactgggc cgccaagggc
     3001 ccctccctcc tgggcgtccg ggccgtgatc gccgagagct acgagcggat ccaccggagc
     3061 aacctggtgg gcatgggggt cctcccgctg cagttcaccg acgggcgcac cgcggagacg
     3121 ctcggactca cggggcgcga ggtctacgcg gtcaccgggc tccgggccgg cctccggccc
     3181 cgacagacgg tcacggtcac ggcccggggg caggacggcg aggaggtgtc gttcgaggct
     3241 ctggtccgga tcgacgagga ggcggaggtc gacaccttcc gccacggcgg gatcctgcag
     3301 atggtcctgc gccagctgct ccgggcctga gcggtccgct gggtccgggc ctgcgcggcc
     3361 cgtgccctcc cgccgcgatc gcagcgctcc ggtccggccc gactagcatg tccgccatgg
     3421 agctgtcggt cggtctgccc cgcatgcaca aggaagcggg cgagcgccgc gacttcctcc
     3481 ccggcttcgt cgcgttcctc aagcggaccg ggttcgggga catcgtcatc gagcacggct
     3541 acgggcaggg cgtgggcttc gaggagaaga cctacctcaa gcgatccgaa cgcgcccggt
     3601 tcgcctcgct cgaggagtgc ctggcccagg acgtgctcgc cgtgatccgc tgcccggacg
     3661 aggaggcgat ccggcggatg cggcggggat cgatcctcct cacgatgatc cactacccca
     3721 cccgaccgga gcgggtggag ctgctgaccg agctggggat ccacggggtc agcctggacg
     3781 gcatcaccga cgatgcgggc cgtcgcctgg tggagaacct ccaggcggtg ggctggaacg
     3841 gggtccacgc cgccttccac gaggtggcgt ccagccatcc ccgtttcgcc catcccagcc
     3901 gccggccgat ccgggtgacg tgcctggggg ccggggccgt ggccgggtac gcgatccggg
     3961 cggccacccg ctacggggat cccaagctcc gggaggaact ggtggccagg cacgtgccgg
     4021 gggtcgaggt caccgtggtc gacttcgacc tcacctggca cgagtactac atgctgaccc
     4081 ggctggagca gaccgacctg ctgatcgacg ccacccagcg caccgatccg agccgcgtga
     4141 tcgtgcccaa cgactgggtg gccgcgctgc cgcaggacgc ggtgatcctg gacctctcgg
     4201 tcgaccccta cgacttctcg gtggacccac cgaaggtgaa gggcatcgag ggcgtgcccg
     4261 agggcacgct ggaccagtac gtcttccatc ccgaggaccc cgcgtacgag cggatggacc
     4321 cccggatccg tcgcgggcac cgccgggtgg cgctgtcgtg ctactcgtgg ccggggatcc
     4381 agccccgaga gtgcatgcag gtctacggca agcagctcga gccggtgatg cgggtgatcg
     4441 cccgcaagcc ggtccagacg tgggacgcgc accacggcac ccactacgag cgggcggtgg
     4501 ccagggcgga ggtgaaccgg tggcgggtcg agaacgtgcg ctgacgttcg gtctgccccg
     4561 gatgcacaag gaggcggggg agcgccggga cttcctgccc gctctggtgg gcaagctcac
     4621 cgatcgcggc gccgaggtcc tggtcgagcg gggcatcggc tcggggatgg gcttccgcga
     4681 ccgcgactac gcggcgctgt cccggctggt ccggttggtc acgaaccggg aggcgttcgg
     4741 ggccgacgtg gtcctcaccc tccggtgccc ggacgagtcg gagttcacga agctccgctc
     4801 cggggccctg ctcttctcga tgctgcactt ctccacccgg cccaagcgcg tgcgccggtt
     4861 gcacgacctg ggcctggagg ccatcagcat ggactcgatc accgacgatc atggtcgtcg
     4921 gttggtggag gatctgaagg ccgtggcctg gaacgggctg gaagcggcct tcgaggcgct
     4981 ggccagtggc tggccggacc tcgagcgccc cgaccgaggc ccggtgcagg tgacgatcat
     5041 gggtgccggg ggcatcggca aacacgccgt ggaggcggcc acgaagttcg gtgacctcgc
     5101 acgcaacgcc cggtacgcgg aggccgacct gtccggtgtg caggtcgtga cggtgggccg
     5161 caacgtcacc gggcgggaac agcacctgcg ggcgctcctg cgccgcaccg acgtgctggt
     5221 ggacgcgacg caccgggacg agccgtggcg cccgctggtg ccgaacgcgt ggatcgccga
     5281 cctgcccgag cacgcggtga tctgcgacct ggtcgtggac ccgtacctgc cggaggggga
     5341 cccgcccacc gtccggggga tcgagggcat cccgcagggg aacctggacg ggtgggtctt
     5401 cgtgcgcgac gatccggtct gggacctggc gccgccatcg gtctccaccg agcaccgccg
     5461 gacggtggtg agctgctact cgtggcccgg cgtccacgcc gtgccgtgca tggagcagta
     5521 cggcacgcag ctggccccgc ttctggagga gctgctgcgg gccggtggca tggacggcct
     5581 gcgccccgag ggggcctacc atgagcgcgc gctgtaccgc gcgagcctgc gcgcctggct
     5641 cgcggtgggg tgacgccgag cccgggcgcc cgaccgatgg tgggccggcg ctcgccctca
     5701 ggcccggggt tccgccgtcc ccatgcgcca gtcgtacggg ctcgtgtagc tcacgatcat
     5761 ctgcatatag ttggcccggg cgaaggcggt gggatcggcc acgttggcct ggctcacgct
     5821 gcccttcatc tgctcgaccg agacgtactc gcgctcctcg agccaccgct ccaacccggc
     5881 caggacgctg gccacatgct cgggcccgtt gcggaacagg gccgaggcca tcatcgtgac
     5941 gtcggcgccg gccagcagca gcttcaccga gtcctcccag gtgtgcacgc cggtggtcgc
     6001 ggccagcgag cagcccaggc ggccccgaag gatcgcgatc caccgcagcg ggagcaggca
     6061 ttcatcggag gtggacaggt gcagcgtggg atcgacctcc agcgtttcca ggtcgatgtc
     6121 gggctggtag aagcggttga acagcaccag cccgtccgcc cccgctcggt ccagctgccg
     6181 cgccatgttc gccggggagc tgaaggacgg cccgatcttg acggccagag ggatccccac
     6241 ctccgagcgc acctccgaca ccaggtccag gtagcggcgc tcgacctgct ctccggtctc
     6301 gtcgggatcg gccgcgatga agtagacgtt gagctccagg gcgtcggccc cggcctgctc
     6361 gatcagcttc gcgtagcgga cccagccccc gggcgaggcg ccgttcaggc tgccgatcac
     6421 cgggatctgc agctcccgct tggtcatctc caggtggcgc aggtactcgt cggggccggt
     6481 gttgtagtcc tcgagctccg ggaagtaggt gagcgcctcg gcgaagctct cggcgccggt
     6541 ctcgagcacc cggaagatct ccagctcgtc gtgctcgatc tgctcctcga acagcgactg
     6601 catcaccacc gcggcggctc ccgcgtcctg catccgcttc aggttgtcgg tgcgtgagga
     6661 cagggtcgag gccgagggga cgatggggtt cgcgagctcc aaccccaggt aggtggtccg
     6721 aaggtcgatg ctcatgccga cccctccttc ccgccgtccc ggcctcgctt ctcgtgcccg
     6781 ttcagctcgc cgggggcgtg ccgctgcacg ccggccagct gctcgtacag gcgccagcgc
     6841 tcctcggcgt cctcctgagc gagctcctgc agctcgccga actcctcggg ccgggatcgc
     6901 gcgagcatgg cgaaccgcgc ctccttggcc tggaagtctt tgagcttcaa cgacggggcc
     6961 ttgctgtcca gatgcagggc ccgctccgag tccgtggcct tgcgcgggtc gtaccggtac
     7021 agcggccaga acgccgactt cacgatctcg gcctggtgcg tcagcgacgt cgacatgtcg
     7081 atgccgtgct cgatgcaggt gctgtaggcg atgatcaggg acgggccgtg ccaggactcg
     7141 gcctcggcga aggccttgac cgtctgggcg ttgtcggcgc cgatcgctac ctgggccacg
     7201 tacacattgc cgtactcggc ggcgatcatc cccaggtcct tgcgccccac ggccttgccg
     7261 cccgcggcga acttcgccac ggccccccga ggggtggcct tggaggcctg gccgccggtg
     7321 ttcgagtaca cctgcgtgtc caggaccagc acgttgacgt cgcggccact ggccaggacg
     7381 tggtccagcc cgccgtagcc gatgtcgtag gcccagccgt cgccgcccac gatccagacg
     7441 ttcttgcgga ccagcacgtc ggccacggcc agcaggtccc gggcctcggg cgactcgagt
     7501 ccctccagtc gccgcttcag ctcggccacg cgctcgcgct gctcggcgag gcccccctca
     7561 tcggtctcct cacgcgggtc ggcggccagg atcgcctcga ccaggtcctg gcccagctcc
     7621 ggcgcgagct tcggcagcag ctcccgagcc tgggccagct gctgatccag ggccagccgc
     7681 atcccgagcc cgaactccgc gttgtcctcg aacagcgagt tcgaccaggc cggcccgcgc
     7741 ccgttcacgt ccttcgacca gggcgtggtg ggcaggttgc ccccgtagat cgaggagcag
     7801 ccggtggcgt tcgcgatcat gatccggtcg ccgaacatct gcgacagcag cctgaggtac
     7861 ggcgtctcgc cgcatccggc gcaggcgccc gagaactcga acagcggctc cagcatctgc
     7921 gagcccttga tcgaggccac cttgacctcc cgccggtcgg ggcgggggat cgtctggaag
     7981 aactcgaagt tctcccgctc ggcctccagg tgctcgagct tgggcagcat gtcgatcgac
     8041 ttgtgcttga cggcctcctt gctcttggcc gggcagatgt ccacgcacac cccgcaaccc
     8101 gtgcagtcgt cgggcgccac ctggatcgtc atccagcggc cgggatgctc gcggtcccgc
     8161 cactccttgt gctggaaggt gtccggggcc ccctcggcat ccgaggggtc gtagaccttc
     8221 atgaggatcg cggcgtgggg gcagaccagc gcgcacttgg cgcagtcgat gcagatcgac
     8281 gggtcccaga tcgggatctc gtgggcgatc gagcgcttct cccacctggc cgtgccggtg
     8341 gggaaggtgc cgtcgggcgg gagagccgag accggcagca ggtcgccctg gccggcgatc
     8401 atcatggccg tgacccgctt cacgaagtcg ggcgcgtcgt cggggaccgg aggaagacgg
     8461 tgcaggtcgc cggacgtctc cacggggatc cgtacctcgt gcaggccttc cagggcccga
     8521 tccaccgccg cgtagttcat gtccagcacg ctctgccccc gcttcccgta gctcttctcg
     8581 atggcctgct tgatccgggc gatcgcctcg ggctgatcga ggatcccggc cagggcgaag
     8641 aagcaggtct gcaggatcgt gttgatgcgc gtgccgaggc ccacctcgtc cgcgatcgtc
     8701 tgggcgtcga tcacgtagaa cctcagcccc ttgtccacga tcgcgcgctg ggtctccagg
     8761 ggcaggtgcc cccacacctc gtcagggccg taggggctgt tcagcaggaa cgtggccccg
     8821 ggctcggccg cggccagcac gtcggtgcgc tccaggaagt tgaactggtg gcaggccacg
     8881 aagccggcct gccggatcag gtaggcgccc tcgatgggcc ggggcccgaa gcgcaggtgc
     8941 gaggtggtca tggagccgga cttcttcgag tcgtagacga agtagccctg cgcgtacagg
     9001 tccgtgccct cgccgatgat cttgaccgag ctcttgttcg cgctcaccgt gccgtcggcg
     9061 cccaggccgt agaacagcgc ccggaccacg tccttgggct cggtgcggaa cgtcgggtcc
     9121 cagctcaggc tggtgtggct cacgtcgtcg tggatgccga tcgtgaagtg ccgcttggcc
     9181 tcgtccttgg ccagctcgtc gaagaccgcc ttggccatgg gcgggttgaa ctccttggag
     9241 gacagcccgt accggccacc gatcacgcgc gggacggccc ggagccggcc cgccgccccc
     9301 tcctccgcga acgccgtcag gacgtcctgg tacaggggtt cgcccagcgc cccgggctcc
     9361 ttcgtgcgat cgagcaccgc gaccgacgtg acggtcggag gcagggacga gacaagcgcg
     9421 gccgccgaga agggccggta caggtggacc tcgagaacgc cgaccctctc cccgtcctcc
     9481 accaggcgct ccaccgtctc acggacggcg cccacgcccg agcccatgag cacgaccacg
     9541 cgttcggcat cgggagcgcc cgagtagtcg aacaggtggt agtggcgccc ggtgagctcg
     9601 ccgaaccggt ccatcgtgta ctgcacgatg tccggcacca cctggtagaa ggggttcgcg
     9661 gcctcgcgcg cctggaagaa gacatccggg ttctgcgccg agcctcggag caccgggtgg
     9721 tccggggaga gggcccgctc gcggtgcgcc cggaccaggt ggtcgtccat cagctcccgg
     9781 agcacgtcgt cctccacggc ctcgatcacg ttcacctcgt gcgaggtgcg gaagccgtcg
     9841 aagaagtgca ggaagggcac gcgggcctcg agcgtggaga cgtgcgcgac cgcggccatg
     9901 tcctgcgccg actgcaccga ggacgaggcc agcatgcccc acccggtggg acggacggcc
     9961 atcacgtcgc tgtggtcgcc gaagatcgac agggcgtgcg tggccacggt gcgcgccgcc
    10021 acgtggatca ccgccggggt gagctcgccg gcgatcttga acatgttcgg gatcatcagc
    10081 aggaggccct gggaggccgt gaaggtggtg cccagggcac cgccctgcag ggctccgtgc
    10141 agggcgccgg ccgccccgcc ctcgctctgc atctcgatca cctggggcgc cacgccccac
    10201 aggttggtcc gccccgccgc cgaccacgcg tcggcgtgct cggccatcgg ggaggacggc
    10261 gtgatcgggt agatggcgat cacctcgctc agcttgtggg cgatgcgcgc cgtcgcctcg
    10321 ttcccgtcca ccgtgaccaa cgtcttgcgt cccgtcatcc cgctcaccat ccgtctcgtc
    10381 ccgccggccg tccgatgccc cctcgacgtg agggcggggg cacaggcccc cgccctcagc
    10441 gtctcgtcgt ctgggctgtc agctcaagcg agctgcctgg tcggctcctt caccctgtgg
    10501 aacaacctac cgggctccca cggcgtcccg agggccggaa ggaccacccg gtccaggccg
    10561 atgtacccgg cgttccgcca cgcgaggacc agcaggacct cggccaggaa gaacacgggg
    10621 ttcacgccgg ccacgccggc cagcccgaag gacatgctca gcatgcctgc gaagaaggcg
    10681 gagacgccgg tgaacagccc gacgatcagc gcgatcccga tcgccatctc gcccagcggg
    10741 atgacccggg tcatgatccc ggcgccgtcg ccacccaccc agtgcaggaa cgacgcgtac
    10801 cagccgtagt tgacggcgga gtggggcccc tgcgcggcgc cgtggagcgc cccggcggag
    10861 aaccccgcga tgcctgccga actgcccagc cacccgccct ggatcttctc ccacccggcg
    10921 ttggcccact cgtacccgaa gtacacgcgg accaccagcc agatccagga ggcagcggtc
    10981 gaatcgaaga gccacttcgc gaacctgggc tcctccaagt gcacggcctc tgatgccatc
    11041 gcaccctcct ttctacggct gccagcatgg ccggagagcg gtggagcgcc agcggtcggt
    11101 cgcccccttg ccaaatagga ctttcccggt ccgggatggg acctttggcc ccccggcgac
    11161 gtccggtgcc gagcgtggtg aacgcgggcg ctaggactgg gtgcctgggg tggtcgtgtg
    11221 gcgcgccagg taggcggcgg cctcggcccg gcgggccacc tcgagcttgg acaggatgct
    11281 cgagacgtag ttcttgacgg tcttctccgc caggcggagg tcctcgccga tctctttgtt
    11341 cgtcttgccg tcggcgacca gggagaggat ccgctcctcc tggggggaga gccgggcgag
    11401 cttctcgtcc ttcagcaggt gcttgccctt gcgtaggcgc tccagcacgg cgttcgtgac
    11461 cgcggggtcc aggaggctct cgcccttgcc caccgtgcgg atggcccgga ccagctcgcc
    11521 ccccttgacc tgcttgagga cgtagccgga ggcccccgcc atgatcgatg cgaacagggc
    11581 ctcgtcgtcg gcgaaggacg tgagcatgac caccgaggtc ttgggatgcc tggcccggat
    11641 ctcgcgggtg gcctcgatcc cgcttccgtc ggccaggcgg acgtccatca cgaccacgtc
    11701 ggggcgcgag cggtcggcct cgtcgatcgc ctcgcggatc gtccccgcct cggccgtcac
    11761 gatgatgtcc tcggtggcct gcaggagcgc cttcacgccg ctgcggacga cctcgtgatc
    11821 gtccaccagc agcacgcgga gcggcggttc cttggtgccc ttcgtcacgt gagccatcct
    11881 agcggctggg cgccgccggg cacgtgaagg cggggccttc agctcgctca cgcacccgtc
    11941 ccggtagcgg cgggcaggcc gttgcggagc atctgcccca cgccgtccag gaagtccttc
    12001 acggtcgcct cggccacccg gtgcaggaga gcccggtcca ggacgcgacc gagcgccccc
    12061 aggggcgggc ggtaccgccc gctgagcgac agctgggtgc ggtcggcgcc cagaccggcg
    12121 atctccatgt ccgcctccag gatggggaac agcccctcgg tccgggtggc cctccaggtg
    12181 agcggcagca ggaccttgcc cgggctccgg taggggttgc cgagctcgat ctccacgctc
    12241 ttggccacgc gccggtcgct ggccgggaac cccacgtcga ccaggagccg ttcgccccgg
    12301 tcctcggcac cctcggcgat gcccgggagc cacgaggccg ggtcgtgcag cagcttctcc
    12361 tcgacctcgg cgaaccgcat ggccaggtcc aggtagtaac ggatgaacat gt
//
LOCUS       DASQQQ010000029        21395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_388040, whole genome shotgun sequence.
ACCESSION   DASQQQ010000029 DASQQQ010000000
VERSION     DASQQQ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 21395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21395
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388040"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VE646_01550"
     CDS             <1..559
                     /locus_tag="VE646_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013020730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZD78709.1"
                     /translation="VVSVDGVPLPQLTEAQLRRVVAMVTQENFLFTGTVAENIAFGRP
                     DATAGEIEDAARAIGAHAFIEALPNGYDTQVEKRGSRLSAGQRQLVAFARAFLADPAV
                     LILDEATSSLDVPSERAVQRALRTVLVDRTALIIAHRLSTVEIADRVLVLEDGRVVED
                     GRPTELIAAGGEYADLHQAWRESLL"
     gene            complement(591..1493)
                     /locus_tag="VE646_01555"
     CDS             complement(591..1493)
                     /locus_tag="VE646_01555"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZD78710.1"
                     /translation="MERRRLGRLEHRSSVLIYGGAALSEVPQDVADDSIALALRAGIN
                     HFDTAADYGDSELRLGAWMPKIRDEIFLATKTGERTAHGAYDGIRRSLVRLRVDRVDL
                     LQLHAVGTIEQLDAVTGPHGALEGALRARDEGLVGAIGITGHGMRAPATHREALRRFS
                     FDTVLTPYNYRLSLEREYVDDFRELLETVRAQDVGLMLIKALARNLWRADRTDRRATW
                     YEPLEDRPHIDAAVAFALAVPQATGITTPGDVALLPAVIGAERGAAETAADSAAAVLA
                     EIPDLEPPFVRVAGRSYPDWLDEP"
     gene            1604..2242
                     /locus_tag="VE646_01560"
     CDS             1604..2242
                     /locus_tag="VE646_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78711.1"
                     /translation="MKLRRVRWVPVAVAVAVGLGWWFGGFREAFYGSSHDGRVPTAEP
                     SPSPTAAPASPSTPSTFASTREIARGRALGVAWAITASSGSGPTCIIIQRPSSRHSKT
                     CWPADRVSTEAPLRAAAPRGEFWFGPAPIAAADVWVTLSDGRTREGTIHHIPSASVGW
                     GSVYLVDVPFPRASGTVVVVGTEGRFLTAAGTAAHRVIPLVRLYSDRLRPPS"
     gene            2352..2546
                     /locus_tag="VE646_01565"
     CDS             2352..2546
                     /locus_tag="VE646_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78712.1"
                     /translation="MRGGIVLAVLGLMAVLWATVARPGATGGTVLLIGYLLLVTVYFI
                     GYRWAWPRWRDRHAKRVAGR"
     gene            2652..4100
                     /locus_tag="VE646_01570"
     CDS             2652..4100
                     /locus_tag="VE646_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HZD78713.1"
                     /translation="MAEPRVSRYAWVTMGVVLIGTFMVFLDTTIVNVALPQIGFDLHS
                     GQGIEWIITGYLLAVGVSQPASGWLADRFGRKKIYTLSLAAFAGGSLMSALAPNLQAL
                     VGFRVFQGLGGGAMMPVGLAMIYELFPPTRRGTALGIWGIAAMAAPTVGPAAGGYIVT
                     AFGWRWLFLLNVPIGAVGIVAALRLLRASAGFHEERPFDGVGWGLGTAGLLLLLLAFS
                     EADSWGWSSAAMIGSIAGGLALLALFVRRELRTDDPLIDVRMFAVPVFSLTMILVALI
                     TTIQFGRLVFVPIDLETVRRYSAFVTGLILTPSAIGTALTMPLGGKLADLVGARTPVL
                     AGVVILTLAAWFLGHLRAGSSVTWIILVLVIQGAGTGLALMPNTVTAMNALPARLVAQ
                     GSAVRAVNRQIAGSLGTAVLASIVAARIGTVSAGRAVPPAAGQAAYNVVYLIGFGAGV
                     LAIVLAIFLPGRARMRELQRQRSEEFVAPAEA"
     gene            4103..4540
                     /locus_tag="VE646_01575"
     CDS             4103..4540
                     /locus_tag="VE646_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZD78714.1"
                     /translation="MSEIAIVGGGMVGELTALLLAEHGHQVLLLDRDEEPPDGSPEDD
                     FERWPRPGVPQAVHAHVFRARSTRLLREEAPDVLEALEDRGVSAPGSGSARGSSGMPR
                     CPPAVPSTRRCCTARSEAGRGSNVAPAYTSLACWAGRTDTSPP"
     gene            complement(5901..6719)
                     /locus_tag="VE646_01580"
     CDS             complement(5901..6719)
                     /locus_tag="VE646_01580"
                     /inference="COORDINATES: protein motif:HMM:NF016334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HZD78715.1"
                     /translation="MEHRLALDRVYRWAHEDDNIRLVVLTGSLARGEGAADDLSDLDI
                     ELYVRDPSLLLERSDWYRPFGEVLVVEEPENVDWHPTRLVHYVDGKIDFMIAGIDAAI
                     QGVSYDRPFHVLLDKDGLSHGLSRAAGPPLPPSRDEITRCINWFYAAALMSAKCLIRG
                     EPWMAKFRDRDVKDELLQMLVWDHRSRYGWTYDTWHNGGHLREWIDHDLMDAVGSCWG
                     DFSIVGMGRALRASVSLFEELSARTQAALGVQPFDSGPVRAEIDRLLSLPHRTA"
     gene            complement(6722..7522)
                     /locus_tag="VE646_01585"
     CDS             complement(6722..7522)
                     /locus_tag="VE646_01585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HZD78716.1"
                     /translation="MVGIRDSSVNRSRLDVVRALAAAGCVAPADEADELIRAAHAGVA
                     PLDEILVRRERGEPLAWIVGSLEFCGLPIRVEPGVYVPRAHTEPLARRAASLLPEVGV
                     AVDLCTGSGAVAAVMHHARPAATVLGTDIDQIAVACATSNGITALRGDLDDPLPVSLV
                     GLVDVMTAVVPYVPREEMHLLPRDVIAFEPHLALDGGPGGTRFLERVARLSVRWLRRG
                     GHLLLEIGGDQARVLEPTFRALGLSNPGIHRDAEGHDRAIELTLRNGR"
     gene            complement(7605..8687)
                     /locus_tag="VE646_01590"
     CDS             complement(7605..8687)
                     /locus_tag="VE646_01590"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZD78717.1"
                     /translation="MITDEPVRGSYAYVEKPGLFGLSGLDQLRLFTEGRAPGPPIHHL
                     AGLVVVDAREGASTWSMPASPWWRSATGLFPGGALAFVADAALGGAIYTVQPARTSVV
                     TSELSLDFVRPATPSSRTLSATGRVVHVGRRQALSEARIEDAAGALLAHATSRCLVQP
                     VPFDPPELPASWSTLPVADPADPDPYQRPPQGEVLPQAEWDRAGGLELMQRWVAGELR
                     LPPICHLLGWRPLEVDQGAAAWTMPASEWFCTGYATFYGGAVGLLADGAIGTAITTTI
                     PAGAAFGTLDLKVNFLRPVTADGRDLVARGTVVHRGRRIVVATATVEDADGKRLAMAT
                     GSAMILPARPWSTVSRDDLAPNGHED"
     gene            8719..9294
                     /locus_tag="VE646_01595"
     CDS             8719..9294
                     /locus_tag="VE646_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZD78718.1"
                     /translation="MPEILLFHHAYGLTAGCRSFADDLRAAGHVVHLPDLYEGHTFGN
                     LDEGVGYAKQVGFDTIIERGRVSAEDLPNEIVYGGFSLGVLPAQMLAQTRPGARGALL
                     LHACVPPSEFGEGWPEGVPVQIHMMEADPMALEGDLDAARELADGVDGAELFLYPGDR
                     HLFSDRSLPDHEEAAATLMTKRVISFLDGLG"
     gene            9308..9997
                     /locus_tag="VE646_01600"
     CDS             9308..9997
                     /locus_tag="VE646_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78719.1"
                     /translation="MGEGMGSPGGTGAAAGPAVDLHWIPLGAGGYVVRWNGKVYEAMK
                     AGLERRPRYALYHTALEVLVPGGRFAIESAPIPDRRRWERGVVAEGPVGMRLAGRFRL
                     FRYEIRRWESGWIPDIHEAVRTDRVADDAERALRMLDLVPAVPTPVWGRDEFGTGDMW
                     NSNSLTSWLLLRSGIDVDRLEPPPGGRAPGWDAGLVVGRRQMGAARDPRPDGSGHRGR
                     RSTIRIRAGRR"
     gene            10255..10860
                     /locus_tag="VE646_01605"
     CDS             10255..10860
                     /locus_tag="VE646_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZD78720.1"
                     /translation="MGRLFSPGGTRGVILAHQVDDDQTDWLDFAPVLASHDFTVLTFN
                     FEGYCGGGGCSRGTAPSTELWTDIVGAVDFLETKGVRQIGLLGASLGGEVCMAAAARL
                     GKRIGAVATLSASIGLAEVGVAAARRDASAITVPKLFVSGRFDTAPAHAARTFERVAE
                     QPKRLVLLPSGEHGIDLLRWEPGERTRSLILAFFDRALARS"
     gene            complement(11041..11271)
                     /locus_tag="VE646_01610"
     CDS             complement(11041..11271)
                     /locus_tag="VE646_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78721.1"
                     /translation="MNEIEEARHRLETDLRELEERLPPTLRSIKSLVGVLIGSASLGF
                     VVRRLRSRGRSEPGPAAEVVVRIVREDRPQDG"
     gene            complement(11268..11678)
                     /locus_tag="VE646_01615"
     CDS             complement(11268..11678)
                     /locus_tag="VE646_01615"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZD78722.1"
                     /translation="MDERIEGARAGRRPLGTVIASAVDDARTLVRKHVELTRIEVAEA
                     VSARAIGVGLMVAAGVVGLYAIGFAAAAGSAALDLVLPTWAADLAVAAASVLLAGGLV
                     LAGRRAMRTAPTTPKRTQETLKEDARWAKRQLAR"
     gene            11842..12300
                     /locus_tag="VE646_01620"
     CDS             11842..12300
                     /locus_tag="VE646_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZD78723.1"
                     /translation="MSDPQGLERAYREHGQRLWRAVLAYGGDPEIASDAVAEAFAQAL
                     RRGSAIREPERWVWTAAFRIAAGELKERRRRNDQPVADPYVLPDEAIELASVLRTLPD
                     KQRAALVLHYYADLPNDRIAEILGITRATVRVHLSQGRRRLKQLLEDDDA"
     gene            12293..13504
                     /locus_tag="VE646_01625"
     CDS             12293..13504
                     /locus_tag="VE646_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78724.1"
                     /translation="MPDIKERFRAFDRVTSPDLWDEAERRTPRGQVPGEPSAGRRLAV
                     AALALAVAAAGIGVAVRAIGVGTGEEPGASDTSVTPPTPSPSGTAGPTIGGPVSAVLA
                     LPGTSSSIVAGPDGVAYVAVVTDAGSRQIVRSDPSEGSVVTNTAIPGGKQGVSIAFVG
                     DALWAGWGHELHRLDPASLDELNRVALPSAPGAIAVSPEGSLWVGVDGRILLIDPASQ
                     LTTQTVTLDGTARLISFDPSGRHAYVVTDAPVGQDGELLAELDATTGQLISSSAVGYR
                     DLLGPSAIAATDTGVWVSYPTGMMGQVDFLPGKGLTAARPGLGREESSNQVAVSAAAG
                     LVWVSDQAAGTLACIDPTTRDVLDRFSMTTTKTNDLSGRLVTQVGSALYMDGSSSLLR
                     LDPPTRCIPGE"
     gene            complement(13556..14929)
                     /gene="murF"
                     /locus_tag="VE646_01630"
     CDS             complement(13556..14929)
                     /gene="murF"
                     /locus_tag="VE646_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HZD78725.1"
                     /translation="MQGRTTSSIAATVRGRAIGARSWVDHVVIDSRQAGPSALFVALP
                     GTRTDGHDHVDEAYQRGAAATLVSRHDGSGRSAIVVDDPGVALLRLAADERRRLSATV
                     VGITGSVGKTTTKDLLASILGRRRHVTASPASYNNQIGLPLTVLAADRRSEVLVCEIG
                     AGVVGEIASLCQVARPHVGVVTVVGKAHLASFGSVEAVAEGKVELVEGLPRAGTAVLN
                     ADDPVVRSYADRTDAQVLLFGRRPGADVRADRVRVREDGCLGFRLIHQANAANVELRL
                     HGPHFLTDALAASACARTLGIPLEVCADALSHAEPSPRRMQERVTARGLRIWDDSYNA
                     NPTSVAAALAALAALPDRGRRIAVLGHMAELGRWSLREHERVGELAAAARLHRLVVVG
                     PEARPIAIGAHRAGLRAKQVIGCASVDEALEAIRSSAEAGDIVLVKGSRVTGLDRLVD
                     GLDIRVR"
     gene            14973..16622
                     /locus_tag="VE646_01635"
     CDS             14973..16622
                     /locus_tag="VE646_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HZD78726.1"
                     /translation="MNHPAVGSVCSVMRSHGATRPDPSLGLIDAAFQDLGEDFEELAF
                     LAGSMSRRMQRTDAELIRARRHLEGSSLDLLQEQLRWFRSRYDRMRRTALGLRLEARR
                     GRRSIPIMNGLAERKRSLAFQARVLQSTGAALVTSSDWQSPSYEHAVWSMAGRYTGRI
                     TEHADDYKRDRHQDAAAYERAYLREYIDGWERLNLGAVMTASGMAAFTTILGYLTMER
                     LSPGAILVGRSTYHECRDLLRAGPLGSRTREVPEPDTDRLLTTIDATDAGTLFMDTLC
                     NARGIPVPDVARIIQHVGRRGRPFTLVLDNTGRSCSFQPFSLLPCNPSQLRLIVFESL
                     TKYPQFGLDRATGGMIVASPGDARGLDRYREHLGTNVTDSTVWTIPEPNRAILLRRLG
                     RLGRNAALLARRLSEVAEDHPGALEGIGHPSLARHPSHHLLDGSAFPGGFLSLDLRPH
                     LQRTDVYRAMVRSMLEEARRRRVPLAAGASFGFDTTRIYLTAATAACARPYLRVSSGT
                     EHLAAATAVADVLCAAVTGLGGLVSASDESPGRIGLRGGLE"
     gene            16619..17158
                     /locus_tag="VE646_01640"
     CDS             16619..17158
                     /locus_tag="VE646_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZD78727.1"
                     /translation="MREADVHDPDPRTLARARQGDLDAFEELVRSYQADVWRFACHFT
                     RDRALAEDVTQETFIRAFRFLHSFRGDSRFTSWLLRITRNCAMDTIKRYRGRVLPEAP
                     PSAGSPDPQARVELQAALAAVSDEHREPFLLIEVFGLSYQEAADVLSLPVGTVKSRMH
                     RARLALMQALEVQEDAGGL"
     gene            17148..19166
                     /locus_tag="VE646_01645"
     CDS             17148..19166
                     /locus_tag="VE646_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZD78728.1"
                     /translation="MDCDRVRAALSARMDGDRLSPQTTEAVDRHVAGCRGCSAFDRGA
                     WWLRERARLEPAPRVPDLVVAIMTAVREEVRPGDVVLVGPADVRASAPGRRPLTSRLG
                     PLAAALVVGLLAGSLVSGGPWRSPPVSSMASASEVARAVAEAAGRLDAYQATFVITEH
                     PSPNAPAREFSMHLWFQAPERFRLDVTALTRDAGMDGGRSDLHMVIDGSRWALTAPSS
                     CPAGTCPARTTVTRNRIPFTSFTPADLVVPVSTLGDPSRIRVEGRGTVLGHPAIRVRL
                     PFEDARPLFPFLSLGGRWRPFFPQDGVELWLDSATWSPLRYEVFPAPGRAREEWALRF
                     GLPREPPREPIFEVRATSLSRRDPSPGTFAMPEAPRTVDQGARPATMGQLRRALGFEP
                     IVPQHLGGLQLYRAVLPPSPGDRTPPQAVITYSQGLSWLQMGETAMRTDGPYGPVGPQ
                     AEEVSLPGGGVGYYEPATEGQGRRLSIHSADAELYLETNLPRDRLLRIAGSLPIVGVS
                     MPARWTVRRSAQGVRGRVSPRDAAARLSFPLVLPSSVALPPGFTFASTELVKIGAVIG
                     VTAYYQQVSIDPGIGPIRVHEEPSGSLPPASSALQSRVWVRGHIGRWTPRRGRLEWIE
                     DGVYRSIDAPGLALSALLRMADAMRPYDPSSRTSGRATSLPPLTVGAP"
     gene            19163..20401
                     /locus_tag="VE646_01650"
     CDS             19163..20401
                     /locus_tag="VE646_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HZD78729.1"
                     /translation="MKIGNSLRLVAVGSAGFLAGSLLTFSLQGSARIVARPERSTASG
                     VPLPRIVPEEPQTFLAWTPGGLPGSFGRRLDSLRAVRRSVIVASDNTWLTRSESAQGE
                     IVDRPKPGFAIPLEVAAVSPPAYAPFLPPPDRGVALSLAEGQGVLGASSAQLRGLGPG
                     AVLWFGSVRVEVAAVLPDELVGAHELMVSRATGRRIGVRRDRYALIQPRDEATDRSLT
                     REIRPLLPPGLPVRVRAPGETPYFRQGDAVLPPVKIKELFGEFSARPDPARSGYLIID
                     ASWEREHIMTEHVPLLGTVTCNVALFRQIRGVIHELIAEHLASAIQSFSGCYARRFTN
                     RDPDLSISHHSWGIALDINVPQNPFGAPPHQDRRMVRVFERWGFIWGGTFLTPDGMHF
                     EYRRPPSGRLPALSVAPGRR"
     gene            complement(20376..21089)
                     /locus_tag="VE646_01655"
     CDS             complement(20376..21089)
                     /locus_tag="VE646_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78730.1"
                     /translation="MSRTTEYLKSLDRIGVPDVWERAVQTEPPDRSVPPPISPVRRLG
                     VAALALAVFIAGGSLAWVAFRPTSPAPAAGHGPEGLSARPLALPSIAPGASCPTTGTT
                     RITPGAGFSGSVVAQESAHVYLASSPTVELSARDRQADGWYVVKDVWVIDGTYTGPVL
                     IRGGRIDGAGPLLLGWNPTTPRRDGLLVTAGSPSLQTDPSSGWRSVPMGAYLRSPGCY
                     AYQIDGTDFTSFIVFRARR"
     gene            complement(21086..>21395)
                     /locus_tag="VE646_01660"
     CDS             complement(21086..>21395)
                     /locus_tag="VE646_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZD78731.1"
                     /translation="IRDVAAWTWRVAFRLAAAELRGRRDSPSGWAPTSYEMAEPVPEL
                     LAALKRLSPKQRLAVMLHDYADRPTSEVAQALGCSRATVYVHLSQGRRRLQSLLEDAR
                     "
BASE COUNT         2986 a         7530 c         7614 g         3265 t
ORIGIN      
        1 cgtggtgagc gtcgacggcg tcccgctgcc ccagctgacc gaggcccagc tccggcgggt
       61 ggtggccatg gtgacgcagg agaacttcct gttcaccggg acggtggccg agaacatcgc
      121 cttcggccgg cccgacgcca ccgccggtga gatcgaggac gcggcccggg cgatcggggc
      181 ccacgccttc atcgaggcgc tgcccaacgg ctacgacacg caggtcgaga agcgaggcag
      241 ccgcctgtcg gccggtcagc gacagctggt ggccttcgcc cgagccttcc tggccgatcc
      301 ggccgtgctg atccttgacg aggcgacctc ctcgctggac gtgccctcgg agcgtgcggt
      361 ccagcgcgcg ctccgcaccg tgctggtgga ccggacggcg ttgatcatcg cccatcggct
      421 ctccacggtg gagatcgccg acagggtgct ggtcctggag gacgggcggg tggtggagga
      481 cggtcgcccc accgagctga tcgccgcggg tggcgagtac gccgacctgc accaggcctg
      541 gcgcgagagc ctgctctgaa gcacgaggct cccgaaggtg tgaccgcgac ctacggctcg
      601 tcgagccagt cgggatagga tcgaccggcg acccgcacga acggcggctc caggtcgggg
      661 atctcggcca gcaccgctgc ggcgctgtcc gcggcggtct ccgccgcccc ccgctcggcc
      721 ccgatcaccg cggggagcag ggccacgtcg cccggggtgg tgatgccggt cgcctggggg
      781 acggccaggg cgaaggcgac cgcggcgtcg atgtgaggcc gatcctcgag cggctcgtac
      841 caggtggcgc gccggtcggt ccggtcggcg cgccagaggt tcctggccag cgccttgatc
      901 agcatcagcc cgacgtcctg cgcccgcacg gtctccaaca gctcgcggaa gtcgtccacg
      961 tactcccgtt ccagcgagag ccggtagttg tagggggtca gcaccgtgtc gaaggagaag
     1021 cgccggagcg cctcccgatg cgtggccggt gcccgcatcc cgtggccggt gatgccgatc
     1081 gctcctacca gtccctcatc gcgcgcccgg agggcgccct ccagcgcccc gtgcgggccg
     1141 gtgaccgcat cgagctgctc gatggtcccc acggcgtgga gctgcagcag atccacccgg
     1201 tccacccgca gccgcaccag ggatcgtcgg atcccatcgt aggcaccgtg ggccgtgcgc
     1261 tcaccggtct tggtggccag gaagatctcg tcacggatct tcggcatcca tgcccccagg
     1321 cggagttcgg agtcgccgta gtctgcggcc gtgtcgaagt ggttgatgcc ggcgcgcagg
     1381 gccagcgcga tcgaatcgtc cgccacgtcc tgcggcacct cggagagggc cgcgccgccg
     1441 tagatcagca cgctgctccg gtgctcgagc cggcccagcc tacgtcgctc catcgcggcc
     1501 gctcctcgga ggtggcgggt ccatcctacc gacggtgtgg gcggtcggct tcgtcgaggt
     1561 gcccgccggg ccggtgcgga gcgtacgatg ggagcgagac cgcatgaagc tacgccgggt
     1621 cagatgggta ccggtggccg tcgcggtcgc cgtgggcctc gggtggtggt tcggagggtt
     1681 ccgggaagcc ttctacgggt cctcccacga cggccgggtc ccgaccgccg aaccctcgcc
     1741 gtcacccacc gcagctccgg cctcaccgtc cacgccgtcg acgttcgcct cgacgcgcga
     1801 gatcgcccgg ggccgggcgt tgggcgtggc ctgggcgatc accgcgtcca gcgggagcgg
     1861 gcccacgtgc atcatcatcc agcgtccgtc ttctcggcac tccaagacct gttggccggc
     1921 cgaccgggtc tccaccgagg cgccgctgcg ggctgcggct ccccgcggcg agttctggtt
     1981 cgggcccgcc ccgatcgctg ccgcggacgt ctgggtcacc ctcagcgacg gacggaccag
     2041 ggaggggacg atccaccaca tcccctcggc gtcggtggga tgggggagcg tgtacctggt
     2101 cgacgtgccg ttccccaggg catcgggaac cgtggtggtg gtcgggaccg agggccggtt
     2161 cctgacggcg gccgggaccg ccgcgcaccg ggtgatcccg ctcgttcgcc tctattcgga
     2221 ccggctccgc ccgccgagct gagtcgggcg ctgacggccg tgtccgtccg agtgcgccat
     2281 ccggtccccg ctcccaaggc ggctcccatc gggagccgag gctctcaccg ggaacgcatt
     2341 aggctgggag gatgcggggt gggatcgtgc tcgccgtcct cgggctgatg gcggtgctgt
     2401 gggcgacggt ggcgcgtccg ggagccacag gaggaaccgt gctgctgatc ggctacctcc
     2461 tcctcgtcac cgtctacttc atcggctacc ggtgggcctg gccccggtgg cgggaccggc
     2521 acgcgaagcg ggtcgccggc cgctaggcca agcggccccg agcgcggccg aaggctccct
     2581 ccggcgacgc gtgaggggct ccatatactg gctgagggcc tgtcggccgg atccccgcac
     2641 ggaaggagct cgtggcggaa ccacgcgtgt cgaggtacgc ctgggtcacg atgggtgtcg
     2701 tcctcatcgg gacgttcatg gtgttcctcg acaccaccat cgtgaacgtg gcgctgccgc
     2761 agatcggttt cgacctgcac agcggccagg gcatcgagtg gatcatcacg gggtacctgc
     2821 tggcggttgg cgtttcccag ccggcatcag gatggctggc cgaccggttc gggcgcaaga
     2881 agatctacac cctgagcctg gccgccttcg cggggggatc gctgatgtcg gccctcgctc
     2941 ccaacctcca ggcgctggtg ggcttccgcg tgttccaggg gctgggcggc ggcgccatga
     3001 tgccggtggg cctggcgatg atctacgagc tgttcccgcc gacccggcgc gggaccgctc
     3061 tgggcatctg ggggatcgca gccatggccg ctccaacggt gggccctgcg gccgggggct
     3121 acatcgtgac cgcgttcggg tggcggtggc tcttcctgct gaacgtgccc atcggcgccg
     3181 tgggcatcgt cgcggccctg cggctccttc gggcctcggc cgggttccac gaggagcggc
     3241 ccttcgacgg ggtggggtgg gggctgggga cggcgggact gctcttgttg ctcctggcct
     3301 tctccgaggc ggacagctgg gggtggtcct cggcggccat gatcggttcg atcgccgggg
     3361 gtctggccct gctcgccctg ttcgtccgcc gtgagctccg caccgatgat cccttgatcg
     3421 acgtgcggat gttcgccgtc cccgtcttct cgctgaccat gatcctggtg gcgctcatca
     3481 cgacgatcca gttcggccgt ctggtgttcg tgcccatcga cctggagacg gtccggcggt
     3541 actcggcctt cgtgaccggg ttgatcctca cgcccagcgc catcgggacc gcgctgacca
     3601 tgccgctcgg gggcaagctg gccgacctgg tcggcgcccg gacgccggtc ctggccggcg
     3661 tggtcatcct caccctggcc gcctggttct tgggccacct cagggccggc agcagcgtga
     3721 cctggatcat cctggtgctc gtgatccagg gggccggcac gggcctggcc ctgatgccca
     3781 acacggtcac ggcgatgaac gccctcccgg cgcgcttggt cgcccagggc tccgcggtgc
     3841 gcgcggtgaa ccgtcagatc gccggctcgc tggggacggc cgtccttgcc agcatcgtgg
     3901 cggctcgtat cggcaccgtc tcggccgggc gagcggtccc gccggcggcc gggcaagcgg
     3961 cctacaacgt ggtgtacctg atcgggttcg gcgccggcgt gctggcgatc gtgctggcga
     4021 tcttcctgcc cggccgggcc cggatgcggg agctccagcg gcagcgcagt gaggaattcg
     4081 tcgccccggc cgaggcgtga gggtgtccga gatcgcgatc gtcggtggtg ggatggtggg
     4141 ggagctcacg gcgctcctgc tcgcggagca cggccaccag gtgctgctcc tggaccgcga
     4201 tgaggaacct cctgatgggt cccccgagga cgacttcgaa cgctggcctc ggccgggtgt
     4261 gccgcaggcc gttcacgccc acgtcttccg cgcgcgctcc acgcgcctcc tgcgggagga
     4321 ggcccccgac gtgctggagg cgctcgagga ccgcggtgtg agcgcgccgg gatctggttc
     4381 ggcgaggggt tcgagcggga tgccgcgctg tccgcccgcc gtcccgtcta cgaggcggtg
     4441 ttgcaccgcg cggtcggagg ccggtcgcgg atcgaacgtc gctccggcgt acacatcgct
     4501 ggcctgctgg gccggacgga cggacacgtc gccaccgtga cgggcgtgcg gacggacgag
     4561 ggtgagcggg tcccggcaga tccggtgatc gactgccgcg gccggcgctc ctccgctccg
     4621 aggtgactcc gtggcgtcgg ggcacgcctg gcggtcgacg cgctgttccc gtgcgatctc
     4681 cactactacg cgcggcacta ccggctccgg gccggcgcgt cctacccttc tgaggaggtg
     4741 cccgtggggc agtgggcccc gtacgggttg ttcgtcgcgt tcgggggcga caacgggacc
     4801 ttctcgctgg ccggtgccgt gtccaagccg accagacgct gggagcgtgg ttcgaggcgc
     4861 agacggcggt cgacgctcgg atggtccgga cgatgcgcgc cgggatcgcc ggggcgccgg
     4921 cccccgagcc gaggggggat cgtcggaggg aggggacccc tcccccgagt ccggttcgag
     4981 tccctggtcc gcgcctgagg ggcggatccc gccaacccga tctggaggcg ccccaggcct
     5041 ccatctcgtg acccgtggca cccggtcggc aagcgtggcc gccaccaggg cgcacatcgc
     5101 gttcgtgatg gccgccagga acatcacgtg atcgagggca agccccaccc ccaggttggc
     5161 cgagtcgtcg aacgcgttcg ggtcccccgc gaacagcgtc acgacgtgcg ccagctgtgc
     5221 caggttcacg gcgaggaaga ccacgctgaa ccgcgcaaac cacgacgcga tccgtgtcgc
     5281 accacgaccg gggccgcacc tcgggccaca tcctcaccag gaggatgccg accccgagca
     5341 cctcgagggg caggttcggc ctcctcacgc tctggcccat cctggccgtg tccgatcgct
     5401 cccgatgagg ctcgcgggat cgtcgtcgtt ctgaccgtga tcgccgtgct ggctcgctcg
     5461 acatcgcact gacctctgcc ctgaccgccg cggggaggct cgactggctg ctgacggcga
     5521 aaggcggagg cgtgcatctg gccgggctga tggtctggct gagcggccga ggatccggtc
     5581 ggaacgctgt tgccaaacgg ccgatgccgt ctacgatgga ggcacgggag aaaggaggtg
     5641 gtcctacttg catagacagc aggagacccc cgaggtgact ggctgctgag ccatccctcg
     5701 cgggccgggg ccaccgcccc cgcccgtccg ccgggagacc ggcagaatcc aaccagatca
     5761 ccgagcccgc gggctccggt tggtctaccg gaccagcctc gttgtaggcg gcagcgcccg
     5821 cgggcagcgg aactcggtgg gccgggccct gtcacagggc ccggcccatc agcgtcgggg
     5881 gcgcgactgc gcccgcgacg ttaggcggtt cgatggggaa ggctcaacag gcggtcgatc
     5941 tcggcgcgga ccggtccgct gtcgaacggc tggacgccca acgccgcttg ggtccgagcg
     6001 ctcagttcct cgaagaggga gaccgatgcc cggagggccc ggcccatgcc gacgatcgag
     6061 aagtcgcccc agcaggatcc cacggcatcc atcaggtcgt ggtcgatcca ctcccggagg
     6121 tgcccaccgt tgtgccaggt atcgtaggtc cagccgtacc gtgagcgatg gtcccacacg
     6181 agcatctgca gcagctcgtc cttcacgtct cgatcccgga acttcgccat ccagggttcg
     6241 cctcggatca ggcacttcgc ggacatcagt gcggccgcgt agaaccagtt gatgcagcgc
     6301 gtgatctcgt ccctcgacgg ggggagcggt ggtccggcgg ctcggctcaa cccgtggctc
     6361 aagccgtcct tgtcgagcag tacatggaac gggcgatcgt acgagacacc ctgtatcgcg
     6421 gcatcgatcc cggcgatcat gaaatcgatc ttgccgtcga cgtagtggac caaccgcgtc
     6481 gggtgccagt ccacgttctc cggttcctcg accaccagga cttctccgaa cggccggtac
     6541 caatccgacc gctccagcag caggctcggg tcccggacgt acaactcgat atcgaggtcg
     6601 gagagatcgt cggcggctcc ctcgccacgg gccagagacc cggtcaggac caccagacgg
     6661 atgttgtcgt cttcatgggc ccatcggtag acgcgatcga gcgcgagccg atgctccatg
     6721 atcaccggcc gttccggagc gtgagctcga tggcccggtc atggccctcg gcgtcgcggt
     6781 ggatcccagg gttcgagagg cccagagcgc ggaacgtcgg ttcgaggaca cgtgcctggt
     6841 cgccgccgat ctcgaggagc aggtgaccac cccgacgcag ccagcggacg ctgagccgag
     6901 ccacgcgctc caagaacctc gtgccgccgg gcccgccgtc cagcgcgagg tgcggctcga
     6961 aggcgatcac gtcccggggc aacaggtgca tctcctcccg gggaacgtac ggcacgacgg
     7021 ccgtcatcac gtcgaccagc ccgaccaagc taaccggcag gggatcgtcc agatcgcccc
     7081 ggagcgccgt gatgccgttg ctcgttgcac aggcgacggc gatctggtcg atgtcggtcc
     7141 cgagcacggt ggcggcgggg cgggcatggt gcatcacggc ggccaccgcg ccgctgcccg
     7201 tgcagagatc gacggcgacc cccacctcgg gcaggaggga cgcggcccgt cgggccagcg
     7261 gttcggtgtg ggcgcgcgga acgtagacac cgggctccac gcggatcggg aggccgcaga
     7321 actcgagcga tcccacgatc caggccagcg gttcgccacg ctcgcgccgg accaggatct
     7381 cgtccaacgg tgcgacgccg gcgtgcgccg cacggatcag ctcgtccgcc tcgtcggcgg
     7441 gtgccacgca gcccgcggcg gccagggcgc gaaccacgtc caggcgagac cggttcacgg
     7501 agctgtcccg gatccccacc atcctggtcg aggatcacgc gaggcgctcc aaggggaggg
     7561 cggtgaaccc cctgccctca ttggctggtc ctgctccatg gagctcagtc ctcgtgtccg
     7621 ttcggggcta ggtcatcgcg gcttaccgtg ctccagggcc gggcgggaag gatcatcgcg
     7681 ctccccgtgg ccatggcaag ccgcttgcca tcggcatcct cgaccgtcgc cgtggcgacc
     7741 acgatccgac gcccgcggtg gaccacggtg ccccgagcca ccaggtcccg accatcggcc
     7801 gtcaccggac gcaggaagtt caccttcagg tcgagcgtgc cgaaggcggc gccggccggg
     7861 atggtggtgg tgatcgcggt gccgatcgcc ccgtccgcca gcagtccgac ggcgcccccg
     7921 tagaacgtgg cgtacccggt gcagaaccac tccgatgccg gcatggtcca ggcagccgcc
     7981 ccctggtcca cctcgagcgg gcgccatccc agcaggtggc agatcggcgg gagccggagc
     8041 tcgccggcca cccaccgctg catcagctcg agtccgccgg cccggtccca ctccgcttgc
     8101 gggagcacct cgccctgggg tgggcgctgg tatggatcgg ggtcggccgg gtcggcgacg
     8161 gggagagttg accacgaggc ggggagctcg ggcgggtcga aggggaccgg ctggaccagg
     8221 cagcgggaag tggcgtgggc caggagcgct cccgccgcgt cttcgatccg ggcctcggag
     8281 agcgcttgcc tccggccgac gtgcaccacc cgcccggtgg cggagagggt gcggctggag
     8341 ggcgtggccg gccggacgaa gtccagggag agctccgagg tgacgaccga ggtccgcgcg
     8401 ggctgcaccg tgtagatcgc gccgccgagg gcagcgtccg ccacgaacgc gagcgctccg
     8461 cccgggaaca gcccggtggc cgagcgccac caggggctgg ccggcatcga ccacgttgac
     8521 gcgccctccc gtgcatccac cacgaccagg ccggccaggt gatggatagg cgggccaggg
     8581 gcccgccctt cggtgaagag cctgagctgg tccagcccgc tgagcccgaa cagcccgggc
     8641 ttctccacgt acgcgtacga gccgcggacc ggttcatcgg tgatcacgcg ttgcaggata
     8701 acggggtagg ctgcccgcat gcctgagatc ctgctcttcc accatgcgta cgggctgacg
     8761 gccgggtgcc gttccttcgc ggacgacctt cgcgccgccg gccatgtcgt gcatctcccg
     8821 gacctgtacg agggtcacac cttcggcaac ctcgacgagg gggtgggcta cgcgaagcag
     8881 gtcggcttcg acacgatcat cgagcgcggg cgtgtctctg cggaagacct gccgaacgag
     8941 atcgtctacg ggggcttctc gctcggggtg ctgccggccc agatgctcgc ccagacccgc
     9001 cccggcgcca ggggagcgct gttgctccac gcctgcgtcc cgccctccga gttcggtgag
     9061 ggatggcctg agggcgtccc cgtccagatc cacatgatgg aggccgaccc gatggccctc
     9121 gagggcgacc tcgatgccgc ccgggagctc gcggacggtg tcgacggcgc agagctcttc
     9181 ctgtacccgg gcgaccggca cctgttctcc gatcgcagcc tgcccgacca cgaggaggcg
     9241 gcggcaaccc tgatgacgaa gcgggtcatc agcttcctgg atggcctcgg gtagcagcgc
     9301 tccgccgatg ggcgagggga tgggatcgcc gggcgggacc ggcgcagccg cggggcctgc
     9361 ggtggatctt cactggatcc ctttgggcgc gggtggttac gtggtccgct ggaacggcaa
     9421 ggtctacgag gcgatgaagg ccggcctcga gcgtcggccg cggtacgcgc tgtaccacac
     9481 ggccctggag gtgctggttc cgggtggccg gttcgccatc gagtcggcgc cgatccccga
     9541 tcgccgccgg tgggaacgag gggtcgtggc cgagggcccg gtcgggatga ggttggcggg
     9601 acggttccgc ctgttccgct acgagatccg gcggtgggag agcggctgga tcccggacat
     9661 ccacgaggcc gtgcggacgg accgggtggc ggacgacgcg gagcgcgcgc tgaggatgct
     9721 cgacctggtg cccgccgtcc ctactccggt ctggggtcgg gacgagttcg gcaccgggga
     9781 catgtggaac tcgaactcac tcacctcgtg gctcctcctt cgatcgggca tcgacgtgga
     9841 ccggctcgag ccgccgcccg ggggccgggc gcccggctgg gacgccggcc tggtcgtcgg
     9901 ccgccgtcag atgggggcgg cccgggaccc tcgaccagat ggctcgggtc accgtggccg
     9961 gagatccacg atccggatcc gggcaggtcg gcgatgaagc cgtcacggtc gcggcccggg
    10021 ttgaggatgc cgaagcatcg agggcagacg cccatggcct gcgtggtggg cctcatgctc
    10081 ctgtcggtcc tggcggtgag ctgcggcgca ggcggggcca ccggaccacg cggttcccgg
    10141 tcgccgatcg catcgccgat cccgacatcc gggggcccca cgcccagcca gagcgacccg
    10201 ccgacggtgc cgtcgcgccg ggtggtcttc atttcctcgc gaggagcgca cctcgtgggc
    10261 cggctgttct cgcccggagg gacccggggc gtgatcctcg cccatcaggt cgacgacgat
    10321 cagaccgatt ggctcgactt cgcccccgtc ctggcttcgc atgacttcac ggtcctcacc
    10381 ttcaacttcg agggctattg cggaggcggc ggttgttccc ggggcaccgc cccgagcacg
    10441 gagctctgga ccgatatcgt gggggcggtc gacttcctgg agacgaaggg cgtcaggcag
    10501 atcggtctgc tgggggccag cctcggggga gaggtgtgca tggcggcggc ggcgcgcctg
    10561 gggaagcgga tcggtgcggt ggccacgtta tcggcatcga tcggcctggc tgaggtcggc
    10621 gtggctgccg ctcgtcgcga cgcctcggcc atcaccgtcc ccaagctgtt cgtatccggc
    10681 cgcttcgaca cggctccagc ccacgcggct cgcacgttcg agcgcgtcgc ggaacagccc
    10741 aaacggttgg tgctcctgcc ctccggggaa cacggtatcg acctcctcag gtgggagccg
    10801 ggggagcgaa cccgctcact gatcctggcg ttcttcgacc gggccttggc ccgaagctga
    10861 tcgaccggct ggatcacccc ctggtgttcc ggctcaggcc ctggaagggt cggctggcca
    10921 tggcccatga ttccacccag gctcgggcac gatgagcccg cagcgctaca gcgcccgccc
    10981 gcaccaccga tcgccgccgg cctgggaccg caggtagccg ggtactcagg tcggccgacc
    11041 tcagccgtcc tgcgggcgat cctcgcgtac gatccggacc accacctcgg cggccggccc
    11101 gggctcggat cgccccctcg agcgaaggcg ccggaccacg aagccgaggg acgccgaccc
    11161 gatgagcaca cccaccaggc tcttgatgga acggagggtg gggggcaggc gttcctccag
    11221 ctcccggaga tcggtctcca agcggtgtcg agcttcctcg atctcgttca tcgcgcgagt
    11281 tgccgcttcg cccatcgagc gtcctccttc agcgtctcct gcgtgcgctt cggcgtggtg
    11341 ggagccgtcc gcatggcccg gcggccggcc aggacgagcc ccccggcgag gaggaccgat
    11401 gcggccgcca cggccaggtc ggccgcccag gtgggcagca ccaggtccag cgcggccgat
    11461 cccgcggccg ccgcgaagcc gatcgcgtac agtcccacca cgccggcggc caccatcagg
    11521 ccgacgccga tggcccgggc ggacacggcc tcggccacct cgatcctggt cagttcaacg
    11581 tgcttgcgca ccagcgtgcg ggcatcatcc accgccgacg cgatcacggt accgagcggt
    11641 cgtctccccg ctcgcgctcc ttcgatccgc tcatccacga tctggacccc ctcctctcac
    11701 catcactgcc caggacaacc gcaagcaggt tgtactccat accgcggcgg ctctcagacg
    11761 aggatctcgg agcgccggga tgtacacgtt ctggttcaga acgcaccctt ccgagatgtg
    11821 gaacaggatg agctcgtgcg ggtgagcgat cctcagggcc tggagcgagc ctaccgggag
    11881 catgggcagc ggctgtggcg ggccgtgctg gcgtacgggg gcgatccaga gatcgccagc
    11941 gacgcggtgg ccgaggcctt cgcccaggcc ctgcgccgag gctcggccat ccgggaaccg
    12001 gagcggtggg tctggacggc ggcgttccgg atcgcggccg gtgagctgaa ggagcggcgc
    12061 aggcggaacg accaaccggt cgcagatccg tacgtgttgc cggacgaggc catcgagctc
    12121 gcgtcggtgc tccggacctt gccggacaag cagcgggctg ccctggtgct gcattactac
    12181 gcggacctgc ccaacgaccg catcgccgaa atcctcggga tcacccgagc cacggttcgg
    12241 gtgcacctca gccagggtcg gcggcgcttg aagcagttgc tggaggacga cgatgcctga
    12301 catcaaggag cggttccgag cgttcgaccg ggtgacgtcg cccgacctgt gggacgaggc
    12361 cgagcgccgg accccccggg gccaggtgcc cggcgagccg agcgcggggc ggcgcctggc
    12421 cgtggcggcg ctggccctgg ccgtggccgc cgccgggatc ggagtcgcgg tgcgagcgat
    12481 cggcgtcggc accggcgagg aaccgggagc cagtgacacg agcgtgacgc cgcccacccc
    12541 gtcccccagc ggaacggccg ggcccaccat cggtggaccg gtgtccgcgg tgctcgccct
    12601 gcctggcacg tcctcatcga tcgtcgcggg ccccgacggg gtcgcgtatg tcgccgtcgt
    12661 caccgacgca gggtcgcggc agatcgtccg gtcggatccc tcggagggga gcgtggtgac
    12721 gaacaccgcc atccccgggg gcaagcaagg ggtatcgatc gccttcgtcg gcgatgccct
    12781 gtgggcggga tggggacacg agctccaccg cttggatccg gcgtccctgg atgagctgaa
    12841 ccgggtggcg ctgccctcgg ccccgggagc gatcgccgtg tcgccggagg gctcgctgtg
    12901 ggtgggcgtg gacgggcgga tcctcctcat cgatcctgcg tcgcagctca ccacccagac
    12961 cgtgaccctg gacgggacgg cccggctgat ctccttcgac cccagcggga ggcacgcgta
    13021 cgtggtcacc gacgcgcccg tcgggcagga cggcgagctg ctggccgagc tcgatgcaac
    13081 caccgggcag ctcatcagct cgtcggcggt cggctaccgg gacctgctgg ggccatcggc
    13141 catcgcggct accgacaccg gcgtgtgggt cagctatccc accgggatga tgggccaggt
    13201 cgacttcctg cctgggaagg ggctcacggc cgctcgcccc ggcctcggac gggaggagag
    13261 cagcaaccag gtggcggtca gcgccgcggc cggactcgtg tgggtgagcg accaagcggc
    13321 gggaaccctc gcctgcatcg accccaccac ccgcgacgtg ctcgaccgct tctcgatgac
    13381 gaccaccaag acgaacgacc tctcgggacg gctcgtcacc caggtcggat cggccctcta
    13441 catggacggt tcctcatccc tcctccgcct ggacccacct acgaggtgca taccggggga
    13501 gtagccaggc gacgggacac gatgcttcga tccaagggct gaggagcgag ggtccctagc
    13561 ggacccggat gtccaagccg tcgaccaggc ggtcgagccc cgtgacccgg gatcccttga
    13621 cgaggacgat gtcgccggct tcggcgctgg atcggatggc ctcgagcgcc tcatcgacgc
    13681 tcgcgcaccc gatgacttgc ttcgctcgca gaccggcgcg gtgcgctccg atggcgatgg
    13741 gccgagcctc cggcccgacc acgaccagcc ggtgcagccg ggccgcggcc gccagctcac
    13801 ccacccgctc gtgctcccgc agcgaccaac gtccgagctc cgccatgtgg ccgagcacag
    13861 cgatccgccg gcccctgtcg ggcaaggccg ccagcgctgc cagcgccgcc gccacagatg
    13921 tggggttcgc gttgtaggag tcgtcccaga tgcggagccc gcgtgcggtg acccgctcct
    13981 gcatcctcct cggggagggc tcggcatgcg agagggcgtc cgcacagacc tccagcggga
    14041 tccccagggt tcgggcacag gcactcgccg cgagcgcgtc ggtgaggaag tgcggtccat
    14101 gcaagcgcag ctcgacgttc gctgcgttgg cctggtggat gagccggaac ccgagacagc
    14161 catcctcgcg gaccctcact cggtcggccc gaacgtctgc accgggacgc cgtccgaaca
    14221 gcagcacctg ggcgtcggtc cgatcggcgt agctgcgcac caccgggtcg tcggcgttga
    14281 gcacggccgt tcccgcccgg ggcaggccct ccacgagctc gaccttgccc tcggcgacgg
    14341 cctcgacaga gccgaacgag gccaggtgcg ccttgccgac gaccgtgacc acgccgacat
    14401 ggggtcgtgc cacctggcac aaggaggcga tctctcccac cacccccgcg ccgatctcgc
    14461 agaccaggac ctcggagcgg cggtcggccg ccagtacggt gagcggaagg ccgatctggt
    14521 tgttgtagga agcgggactc gccgtgacgt gccgccgacg acccaggatg gatgccagga
    14581 gatctttggt ggtggtcttg cccacggatc ccgtgatgcc cacgacggtc gccgacagcc
    14641 ggcggcgctc gtctgccgcc aaccgcagga gggccactcc cgggtcgtcc accacgatcg
    14701 ccgaccgacc ggacccgtcg tgccgggaga ccagggtggc cgccgccccg cgttggtacg
    14761 cctcgtcgac gtgatcgtgg ccgtccgtcc tcgtgccggg gagggcgacg aagagcgcgc
    14821 tcggaccggc ctgtcggctg tcgatcacca cgtggtccac ccagctccgc gcgccgatcg
    14881 ctcggccgcg gaccgtcgcc gcgatggatg aggtcgttcg tccctgcacg ggtcttcatc
    14941 cctaccgacc ggacggatcg aggagcggtt ccgtgaacca tccggcggtc ggatcggtct
    15001 gttcggtgat gcgctcgcat ggtgctaccc gcccggaccc gtcactcggg ctgatcgacg
    15061 ccgcgttcca ggacctgggg gaggacttcg aggagctcgc cttcctggcc gggtcgatgt
    15121 ctcgtcggat gcagcggacg gacgcggagc tgatccgggc tcggcgccac ctggagggat
    15181 cgagcctgga cctgctccag gagcagctgc gctggttccg ctcccggtac gaccggatgc
    15241 gccgaaccgc gctcgggctg cgactcgagg ctcggcgggg gcgccggtcc atccccatca
    15301 tgaacggctt ggccgagcgc aagcgctccc tggccttcca ggctcgggtg ctccagagca
    15361 cgggggccgc cctggtcacg agctcggact ggcagtcacc ctcctacgag cacgccgtgt
    15421 ggtccatggc cggcaggtac acgggacgca tcacggagca cgccgatgac tacaagcggg
    15481 accgccacca ggacgccgcc gcctacgaac gggcgtacct gcgcgagtac atcgatggct
    15541 gggagcggct gaacctgggg gccgtgatga cggcatcggg catggccgcc ttcacgacga
    15601 tactggggta cctcacgatg gagcgtctgt cgcccggagc gatcctggtc ggccgttcca
    15661 cgtaccacga atgtcgagac ctgctgcgcg cgggtccctt ggggtctcga acccgtgagg
    15721 tccccgagcc cgacaccgat cgcctgctca ccacgatcga cgcgaccgac gcggggacgc
    15781 tgttcatgga caccctctgc aacgcgcggg gcattcccgt gcccgatgta gcccggatca
    15841 tccagcatgt agggcgccga ggacggccgt tcaccctcgt gctggacaac accggccgtt
    15901 cctgcagctt ccagcccttc tcgctccttc cctgcaaccc ttcgcagctc cgcctgatcg
    15961 tcttcgagag cctcaccaag tacccgcagt tcggcctgga tcgtgccacc ggagggatga
    16021 tcgtcgcgtc cccgggcgac gcacgaggcc tggatcgcta ccgcgagcat ctggggacca
    16081 acgtgaccga tagcacggtg tggaccatcc cggagcccaa ccgagccatc ctgctccgac
    16141 gactgggacg gcttgggcgc aatgcggccc tgctcgcgcg ccgactgtca gaggtggccg
    16201 aggaccatcc gggggcgctg gaagggatcg gccatccttc cctggcgaga cacccgtccc
    16261 atcatctgct ggatggatcg gcctttcccg gcgggttcct gtccctggac ctgcgcccgc
    16321 atctgcagcg cacggacgtc tatcgggcca tggttcgctc catgctcgag gaggctcgac
    16381 gccgccgcgt gccgctcgcc gctggagcga gcttcggctt cgacaccact cggatctacc
    16441 tgacggccgc cacggccgcg tgcgcccggc cctaccttcg ggtctcgtcg gggaccgaac
    16501 acctcgccgc agcgacggcc gtggccgacg tgctgtgcgc cgcggtaacc gggctcggcg
    16561 ggctcgtgag cgcttccgac gagagccccg gccgcatagg cttgcgcggc gggctggagt
    16621 gagagaggcc gacgtgcacg atcccgatcc acggaccctc gcccgggcac gtcagggcga
    16681 tctggacgcc ttcgaggagt tggtgcgttc ctaccaggcg gatgtctggc ggttcgcctg
    16741 tcacttcacg cgtgaccggg ccctggccga ggacgtgacc caagagacgt tcatccgcgc
    16801 gttccggttc ctccactcgt tccgaggaga ttcgagattc acgagctggc tcctgcggat
    16861 cacccggaac tgcgcgatgg acacgatcaa gcgttaccgc ggccgtgtgt tgcccgaagc
    16921 acccccctcg gccggttcgc cggatccgca ggcgcgggtt gagctccagg cagccctggc
    16981 ggccgtctcg gacgagcacc gagagccgtt cctgttgatc gaggtgttcg ggctctccta
    17041 ccaggaggcg gcggatgttc tcagcctgcc cgtcgggacc gtcaagagcc ggatgcaccg
    17101 agcccgcctc gcgttgatgc aggccctcga ggttcaagag gatgccggtg gactgtgacc
    17161 gcgtccgggc ggcgctgtcg gcacggatgg atggagatcg gctctcccct cagacgacgg
    17221 aagcggtcga ccggcacgtg gccggctgtc gaggctgcag cgccttcgac cgcggcgcct
    17281 ggtggctgcg tgagcgggca cgtctggagc ccgcaccacg ggtgcccgat ctggtggttg
    17341 ccatcatgac ggcggtgcgg gaggaggtcc ggccgggcga cgtcgtcctc gtcggcccgg
    17401 cagatgtccg agcatccgcg cccgggaggc gccccctcac gtcccggctg ggtcctctgg
    17461 cggcggctct ggtggtgggg ctgctcgcgg gcagcctcgt ctcgggcgga ccgtggcggt
    17521 cgcctccagt gtccagcatg gcctcggcga gcgaagttgc ccgcgccgtc gcggaggccg
    17581 ccggacgcct cgatgcctac caggccacct tcgtgatcac ggagcatcca tcgccaaacg
    17641 cacccgcgcg ggagttctcg atgcacctgt ggttccaggc gccagagcgc ttccgcctgg
    17701 acgtcacggc tctcacccgg gacgccggga tggacggcgg gcgctcggac ctgcacatgg
    17761 tgatcgatgg ctcgaggtgg gccctcacgg ccccctcgtc ctgcccggcc gggacctgcc
    17821 cggcgcgaac gaccgtgacc aggaaccgga tccccttcac ctcgttcacc ccggccgatc
    17881 tggtcgtccc cgtctccacc ctgggcgacc cgtcgaggat ccgggtcgag ggccgaggga
    17941 ccgtgctcgg tcacccggcg atccgggtgc gcctcccgtt cgaggatgct cgccccctgt
    18001 tccccttcct gtccctcggc gggagatggc gaccgttctt cccgcaggac ggggtggagc
    18061 tgtggctgga ctccgcaacg tggtcaccgc tccgctacga ggtgttcccg gctcccggcc
    18121 gcgcgcggga ggagtgggcg ctgcgcttcg gccttcctcg ggaaccgccc cgggaaccga
    18181 tcttcgaggt gcgggcgacc agcctgagtc ggcgcgaccc atcccccggc accttcgcga
    18241 tgccggaggc gccacgaacc gtcgaccagg gtgcccgacc tgcgaccatg gggcagctcc
    18301 gtcgggccct cggcttcgag ccgatcgtgc cgcaacacct tggagggctc cagctgtacc
    18361 gggcggtcct cccaccctcc ccgggcgacc gcactccccc gcaggccgtc atcacctact
    18421 cccaggggct ctcctggctg cagatggggg agacggcgat gcggacggac ggtccctacg
    18481 gccccgtcgg tccgcaggcg gaagaggtga gtcttcctgg tggcggggtg ggctactacg
    18541 aacccgccac cgagggccag ggtcgacgcc tgtcgatcca ctcagcagac gccgagctgt
    18601 acctggagac gaatctcccg cgcgaccggc tgctgaggat cgcgggatca ctgcccatcg
    18661 tgggtgtgtc catgccagcg aggtggacgg tgcgccgctc agcccaaggg gttcgggggc
    18721 gcgtgtcgcc ccgggacgcc gctgcgcggc tgagcttccc gctggtcttg ccgagctccg
    18781 tggccctgcc gcccggcttc acgttcgcca gcacggagct ggtgaagatc ggtgccgtca
    18841 tcggggtgac cgcgtactac cagcaagtga gcatcgatcc gggcatcggg ccgatccggg
    18901 tgcacgagga gccatcaggg tccctgccac ccgcttcctc ggccctgcag tccagggtct
    18961 gggtgcgggg tcacatcggg cggtggacgc cgaggcgcgg tcggctggag tggatagagg
    19021 atggtgtcta tcgctcgatc gacgctcccg ggctcgccct atccgccttg cttcggatgg
    19081 ccgatgccat gaggccgtat gacccgtcat cgcggacgtc cggccgtgca acgagcctcc
    19141 cgccgctcac ggtaggtgcg ccgtgaagat cgggaactcc ctccggctgg tcgccgtggg
    19201 ttcggctggt ttcctggccg gctccctcct caccttctcg ctgcaggggt cggcccggat
    19261 cgttgcgcga cccgaacgat ccacggcttc gggggtgccc ctcccacgca tcgtgcccga
    19321 agagccgcag acgttcctgg cgtggacccc gggcggactc cccgggtcct tcggccgccg
    19381 gctcgactcc cttcgcgcgg tccgccgctc ggtgatcgtg gccagcgaca acacctggct
    19441 cacgcgctcg gagagcgccc aaggggagat cgtcgaccga cccaagccgg gcttcgcgat
    19501 ccctctggag gtggccgccg tgagtccgcc cgcatacgcg ccgttcctgc ctcccccaga
    19561 tcgcggggtg gcgctctcgt tggctgaggg ccagggggtg ttgggggcga gcagcgcgca
    19621 gctgcgcggt ctcggtcccg gagcggtcct ctggttcggc tccgtgcggg tggaggtggc
    19681 ggcggtcctc cccgacgagt tggtgggcgc tcacgagctc atggtctcgc gggcgacggg
    19741 gcggcggatc ggtgtccgac gcgatcgcta cgcgctgatc cagcccaggg atgaggcgac
    19801 cgaccgatcg ttgacccggg agatccggcc gctgctaccg ccgggcctcc cggtgcgggt
    19861 ccgggctcct ggcgaaaccc cctacttccg ccagggggac gcggtccttc cacccgtcaa
    19921 gatcaaggag ctgttcggag agttctccgc ccgcccggat ccagcgcgct ccggctacct
    19981 gatcatcgat gcgagctggg agcgcgagca catcatgacc gagcacgtcc cgctgctggg
    20041 gaccgtgacg tgcaacgtcg cactgttccg tcagatccgc ggtgtgatcc acgaactcat
    20101 cgccgagcac ttggcctccg ccatccagag cttctcgggc tgctacgcga ggcgattcac
    20161 gaaccgcgat cccgacctgt ccatctccca tcacagctgg gggatcgcgc tcgacatcaa
    20221 cgtgccgcag aacccgttcg gtgccccccc gcatcaagac cgccggatgg tccgggtctt
    20281 cgagcgttgg ggattcatct ggggcggtac cttcctcacg ccggatggca tgcacttcga
    20341 gtaccggcga cccccatcgg gacggctccc agcgctcagc gtcgcgcccg gaagacgatg
    20401 aagctggtga agtccgtccc gtcgatctga tacgcgtaac agccgggcga tcggaggtaa
    20461 gcgcccatcg gcacgctccg ccaaccgctc gaggggtcgg tctgcaggga cggggagcct
    20521 gcggtgacca acagcccgtc acgcctaggg gtggtggggt tccacccgag caggagcggt
    20581 cccgcaccat cgatccgccc gccacggatc aagaccgggc cggtgtaggt gccgtcgatc
    20641 acccagacgt ccttgacgac gtaccagccg tccgcctgtc ggtctcgcgc cgacagctcc
    20701 acggtggggc tgctggccag gtagacgtgt gcggactcct gcgcgacgac cgatccggag
    20761 aacccggctc cgggcgtgat cctggtcgtc ccggtcgttg ggcaagaggc gccgggggcg
    20821 atcgacggca gggccagcgg tctggccgag aggccctccg gtccgtggcc cgccgcagga
    20881 gccggggagg tggggcggaa ggcgacccag gcgagcgagc cgccggcgat gaacacggcg
    20941 agggccagcg ctgccacccc caaacgccgg accggggaga tggggggcgg gacggatcga
    21001 tccggcggct cggtctgcac cgctcgttcc catacatcgg gaacgccgat ccggtcgagc
    21061 gacttcaggt actcggtggt cctgctcatc gcgcatcctc caacaggctc tgcagacgtc
    21121 ggcgtccctg gctcagatgt acgtacacgg tcgcgcgcga gcaccccagc gcttgggcga
    21181 cctccgaggt cggtcggtcc gcgtagtcgt gcagcatcac cgccaggcgc tgcttcggcg
    21241 acagtcgctt cagggctgcg agcagctcag ggaccggctc cgccatctcg tagctcgtcg
    21301 gtgcccatcc cgagggggag tcacgtcgcc cgcgaagctc ggcggctgcg agtcggaagg
    21361 ccacccgcca ggtccacgcg gccacgtccc ggatc
//
LOCUS       DASQQQ010000030         6566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_359439, whole genome shotgun sequence.
ACCESSION   DASQQQ010000030 DASQQQ010000000
VERSION     DASQQQ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6566
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359439"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VE646_01665"
     CDS             <1..348
                     /locus_tag="VE646_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HZD78732.1"
                     /translation="IGSMTNDWKPERYEDTHRERVLELIERKASGGRVVVEEAPAPGR
                     VQDLMAALRASVEQAKQGRSRAGAGEAGGREPTDLAGWSRDRLYEEAQRRQIRGRSQM
                     SKDELVEAIRRAS"
     gene            complement(360..1181)
                     /locus_tag="VE646_01670"
     CDS             complement(360..1181)
                     /locus_tag="VE646_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HZD78733.1"
                     /translation="MDGVIGRKTRAAMQGELKARASEPEPPALGIDVLDEQAVRRFQA
                     AFGLEEDGLVGSQAQAALRLEHQRTLGADPAVPGSIRRFQREHDLPQTGIIDEATQGA
                     MRAIRSAEEPSTPEDHERAEGWFEQEDGQPRWMLDPADPESVKRFQREHGLVEDGIIG
                     PQTQVAIRAVRAEHARGRTEPDGDQVVSRPYPHRTLASGLFSSTALPVTDPSLSGEIA
                     AIVEALNEDGRVSRAELAERVGARFWGPGRFRRALDVAEREDRIRKAGDRYVLRR"
     gene            complement(1266..1388)
                     /locus_tag="VE646_01675"
     CDS             complement(1266..1388)
                     /locus_tag="VE646_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HZD78734.1"
                     /translation="MRWPWRWTPTDRDSIRRFQREAGLADDGIIGPRTRGALLA"
     gene            complement(1494..1727)
                     /locus_tag="VE646_01680"
     CDS             complement(1494..1727)
                     /locus_tag="VE646_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78735.1"
                     /translation="MLDRATQGALAGLAAEEGVDEELEVLPGLDIADPDSIGAFQEEH
                     GLEPSGELDAPTQGALAWIYRGGWTWTPWTRTA"
     gene            complement(1739..3289)
                     /locus_tag="VE646_01685"
     CDS             complement(1739..3289)
                     /locus_tag="VE646_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD78736.1"
                     /translation="MAIATRKIRTKVSARMDRLPWSRWHWRIVWALGAVWILDGLEVT
                     MKGAVGARLTNPHALGLTAAQVGTVASLYVAGAVSGALFWGYLTDRFGRGKLFMYTLA
                     LYMVAVLLTGLSWGFYSFMSFRFMTGFAIGGEYAAINSAIDELIPARARGWTDLAIDG
                     SYWIGTIIPASLSLIYLDRSIIPLGRGWRTGFFTGLILAFGLLLLRRRVPESPRWLLT
                     HGKREEAERITSQIEQDVEAATGVSNLQELSDNEAIELRERETTGFVEIARTLFKVYP
                     RRTIVGLSLMGTQAFLYNAIFFTYGLVLTTFFHVSDSVVGIYLIPFAIGNFLARSCWG
                     DTSDSVGRRQMISLSYIAPGVLLIGIGYLFSQAMLSTLWMTVGWVAVFFFASAGASAG
                     YLTVSETFPLEIRALAIAFFYAVATGLGGIVGPWLFGTLIGTGNRFEVYFGYLIGPGL
                     MILGGIVEIIWGVAAERRSLESIAMPLSAAEVEGGPGPHGAGVEGPFDLDPTDPRGHL
                     PAPGPAPA"
     gene            3491..3991
                     /locus_tag="VE646_01690"
     CDS             3491..3991
                     /locus_tag="VE646_01690"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZD78737.1"
                     /translation="MRLGSFLMGGLTGAAIAHFFDPDLGRGRRTRLRDQGLAAVRRLG
                     DRAQARARYRAHQAEGELRERFQPGPQHPFPDDRTLEQRIQSEVFGDPDVPKDRLALD
                     VVDGEAVLRGELDSEAEMRALVQRVSEVAGVRRVQNLLHLPGQPAPNKEEALEASERA
                     EKGGGS"
     gene            3988..4479
                     /locus_tag="VE646_01695"
     CDS             3988..4479
                     /locus_tag="VE646_01695"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZD78738.1"
                     /translation="MSTPAISDPEKVNRGSTGVPDIATERVRGDGSAPGGRESVGELV
                     GRIVGGVKDLARHETELAKIEMKEAAAQQARTAGAAGAAGLFGLFALAFMAAAATAAL
                     TYAIPVWAALLTMCGAFGIAALAALSVAAHFNKKAKSGMSAERTRMTLKETLGWARAQ
                     IRR"
     gene            4452..4736
                     /locus_tag="VE646_01700"
     CDS             4452..4736
                     /locus_tag="VE646_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78739.1"
                     /translation="MGQSADQKVTEIRATRAQVDHDLRELEHRLPIPLNGRTIGLLMG
                     AGTAASLVLKVLSWVWKRRREQRARTANVVVHVVDERHGRHDERDDTEVA"
     gene            4814..5242
                     /locus_tag="VE646_01705"
     CDS             4814..5242
                     /locus_tag="VE646_01705"
                     /inference="COORDINATES: protein motif:HMM:NF022219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HZD78740.1"
                     /translation="MEPRPAQDLGNTVDHPAKLLRIASMVRELQEEIRRETLDEAGVD
                     KLRDAQHRILDELQDVLSEGLREELSALAAPAGAGATESELRVVQAQLLGWLEGLFQG
                     IQAAVFTQQMQARAQLEQMRGRNAPAPSGGPQPPAGGQYL"
     gene            complement(5252..>6566)
                     /locus_tag="VE646_01710"
     CDS             complement(5252..>6566)
                     /locus_tag="VE646_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HZD78741.1"
                     /translation="AERALGKVFPDHWSFMLGEIAAYSFIILVLTGTFLAFFYKASSA
                     ETVYQGSYAPLRGLEVSAAYQSVLHLSFDVRAGLVMRQTHHWTADVFIAAIVAHLCRI
                     FFTGAFRRPRELNWLIGVTMLLLALFNGFSGYSLLDDLLSATGLRIMYSVVQSVPVIG
                     TWMAFLVFGGEFPSDQILGRLLVLHIFIVPALLAGLIGAHLALVWHQKHTQFPEPGLT
                     TEDNVVGSRLWPTYATKSLGLFFLLFAAVAALGGLAQINPIWLYGPFHPYQVTSPAQP
                     DWYLGWIEGALRLFPNWTIQGFGFLVPNAFFPAVLLPGVTFGLLYAWPFLEQWLTKDR
                     APHNLLDRPRDRPVRTAVGTTTLSFYIMLFFSASNDLYARWLQLSIGAVTAFLQWVTV
                     IVPLVVGYLTYKICRGLSVSGAPRFSHMPASAMRHRRPAPFESER"
BASE COUNT         1106 a         2251 c         2332 g          877 t
ORIGIN      
        1 atcggatcca tgaccaacga ctggaagcca gagcgctacg aggacacgca ccgcgagcgg
       61 gtgctggagc tgatcgagcg taaggcgtcg ggtgggcggg tggtcgtgga ggaggcgccg
      121 gcccccggca gggtacagga tctcatggcg gccctgcgcg ccagcgtgga gcaggccaaa
      181 cagggccggt cacgcgccgg ggcgggagag gctggggggc gcgagcccac ggacctggcc
      241 gggtggtccc gcgaccgcct gtacgaggag gcccagcgcc ggcagatccg aggccggtcg
      301 cagatgtcca aggacgagct ggtcgaagcg atccgccggg cctcctgagc cccggccggt
      361 cacctgcgca acacgtagcg gtcccccgcc ttgcggatcc ggtcctcgcg ctcggccacg
      421 tccagggctc gacggaagcg tcccggcccc cagaaccggg cccctacccg ctcggcgagc
      481 tcggctcgcg agactcgccc gtcctcgttc agggcctcca cgatcgccgc gatctccccg
      541 gacagcgacg ggtcggtgac cggcagcgcc gtgctgctga acagccccga ggccagggtc
      601 cgatgcgggt agggtcgcga gaccacctgg tcgccatccg gctcggtccg gccacgggca
      661 tgctcggccc ggaccgctcg gatcgccacc tgcgtctggg ggccgatgat cccgtcctca
      721 accaggccgt gctcgcgctg gaaccgcttg acggactcgg ggtcggcggg atcgagcatc
      781 catcgcggct gaccgtcctc ctgctcgaac catccctccg cccgctcgtg atcctcgggg
      841 gtgctcggct cctccgcgga tcggatcgcc cgcatggctc cctgggtcgc ttcatcgatg
      901 atcccggtct gtggcagatc gtgctcacgc tggaaccggc ggatcgaccc cgggaccgcc
      961 ggatccgccc cgagggtgcg ctggtgctcg agacggagcg ccgcctgcgc ctgggagccg
     1021 accagcccgt cctcctccag gccgaacgcc gcctggaagc ggcggaccgc ctgttcgtcc
     1081 aggacgtcga tgccgagggc ggggggctcg ggttccgagg cgcgggcctt cagctcgccc
     1141 tgcatcgccg cgcgggtctt gcgcccgatc accccatcca cgtccaggtc gtgctcgcgc
     1201 tggaagcgct agatggatcc acggtccgcc gggtccacgt ccaccagccg ggccagcatc
     1261 tgccgctacg cgaggagggc cccgcgcgtc cgaggaccga tgatgccgtc gtcggccagt
     1321 ccggcctcgc gctggaagcg ccggatcgaa tcgcggtccg tgggggtcca cctccacggc
     1381 cacctcaccg ggctcctcct cggggtgctc ggcccggacc gcgaggatcg cggcctgcgt
     1441 cgcgggaccg atgtccccgt ccccctcgag gtcgtaccgg cgctggaacc gcttcacgct
     1501 gtccgggtcc agggggtcca ggtccaaccg ccgcggtaga tccaggccag ggccccctgg
     1561 gtcggtgcat ccagctcacc ggagggctcc agaccgtgtt cttcctggaa ggccccgatc
     1621 gagtcggggt ccgcgatgtc gagccccggt aggacctcca gctcttcgtc gaccccttct
     1681 tccgccgcca accccgccag cgcgccctgg gtcgcccggt cgagcacccc ggtcaccttc
     1741 aggctggtgc tggtccggga gccgggagat ggccccgggg atcggtggga tccaggtcga
     1801 agggcccctc cacgcctgcc ccgtggggac ccggaccgcc ctcgacctcg gccgccgaca
     1861 ggggcatggc gatcgactcc agcgatcggc gttcggccgc cacgccccag atgatctcga
     1921 cgatcccccc gaggatcatc agcccgggcc cgatcaggta cccgaagtag acctcgaacc
     1981 ggttgccggt cccgatcagc gtcccgaaca gccatggacc cacgatccct cccaggcccg
     2041 tcgcgaccgc gtagaagaac gcgatggcca aggcgcggat ctccaggggg aacgtctcgc
     2101 tcaccgtcag gtagccggcg ctcgccccgg ccgaggcgaa gaagaacacc gccacccacc
     2161 ccacggtcat ccatagggtg gacagcatcg cttgtgagaa gaggtagccg atcccgatca
     2221 gcagcacgcc gggggcgatg tagctcaggg agatcatctg ccggcggccc accgagtcgg
     2281 aagtgtcgcc ccaacaggat cgggccagga agttcccgat cgcgaagggg atcaggtaga
     2341 tgcccaccac cgaatcggac acgtggaaga acgtggtgag gaccagcccg taggtgaaga
     2401 agatggcgtt gtagaggaac gcctgcgtcc ccatcaggga caggcccacg atcgttcgcc
     2461 gcgggtagac cttgaacaag gtccgggcga tctccacgaa cccggtggtc tcccgctcgc
     2521 gtagctcgat ggcctcgttg tcggacagct cctgcaggtt cgagactccg gtggcagcct
     2581 ccacgtcctg ctcgatctgg ctggtgatcc gctcggcttc ctcccgcttg ccgtgggtga
     2641 ggagccaccg cgggctctcg ggaacgcgtc ggcgcaggag cagcaggccg aacgccagga
     2701 tgaggccggt gaagaagccg gtgcgccagc cccggcccag cgggatgatg ctgcggtcca
     2761 ggtagatcag ggacaacgac gcggggatga tcgtgccgat ccagtagctc ccgtcgatag
     2821 ccagatccgt ccaccctcgc gcccgagcgg ggatgagctc gtcgatcgcg gagttgatgg
     2881 ccgcgtactc gccgccgatc gcgaacccgg tcatgaaccg gaagctcatg aacgagtaga
     2941 agccccacga caggccggtg agcaggaccg ccaccatgta cagcgcgagg gtgtacatga
     3001 acagcttccc cctgccgaac cgatcggtga ggtaccccca gaacagcgcg cccgagacgg
     3061 cgcccgccac gtagagggag gccacggtcc cgacctgggc cgccgtgagg cccagcgcgt
     3121 gcgggttcgt gaggcgggcc cccacggcac ccttcatcgt gacctcgagg ccgtcgagga
     3181 tccacaccgc ccccagggcc cacacgatcc gccagtgcca gcgcgaccag ggcagccggt
     3241 ccatcctggc cgagaccttg gtcctgatct tccgtgtggc gatcgccatg gctaccccga
     3301 tgggtgggcc gttctccggc gcagtgatta cctgttcacg ctggaacaga cttccggtcg
     3361 ggcgggaccg ggcacatgat cgctgcaccc ccacgtttcg tggaccccag gaagcgggga
     3421 tacccctggt tggacaggca ctcgagcttc ggcatcgacg atcccgcgaa cgggcaagga
     3481 gggatggacc gtgcgcctcg gcagcttcct catgggcggt ctcacggggg ccgccatcgc
     3541 ccacttcttc gaccccgacc tggggcgtgg gcgccggacc cgcctgcgcg atcaggggct
     3601 ggccgccgtg cggcggctcg gcgatcgagc gcaggcccgg gcccggtacc gagcgcatca
     3661 ggcggagggc gagcttcgcg agcggttcca gccggggccg cagcacccct tccccgacga
     3721 ccgcacgctg gagcagcgga tccagagcga ggtcttcggg gaccccgacg tcccgaagga
     3781 ccgattggcc ctcgacgtgg tggacggtga agcggtcctg cggggggagc tggactcgga
     3841 ggcagagatg cgcgccctcg tccagagggt ctccgaggtg gccggcgtgc gtcgggtgca
     3901 gaacctgctg cacctgccgg gacagccggc gccgaacaag gaggaggcgc tcgaggccag
     3961 cgaacgagcg gagaagggag gcggatcgtg agcacgccgg ccatcagcga ccccgagaag
     4021 gtcaaccgag gcagcaccgg ggtgccggac atcgcgacgg agcgggttcg gggggacgga
     4081 tcggcgccgg gcgggcgcga gtcggtgggg gagttggtgg gtcggatcgt gggaggcgtg
     4141 aaggacctgg ctcgccacga gaccgagctg gccaagatcg agatgaagga ggccgcagcc
     4201 cagcaggccc ggaccgccgg ggcagcgggt gcggccggac tgttcggcct cttcgcgttg
     4261 gccttcatgg cggccgccgc cacggcggcc ctcacctacg cgatcccggt gtgggcggcc
     4321 ctgctcacga tgtgcggcgc tttcgggatc gcggccctgg ccgcgttgag cgtggcggcc
     4381 cacttcaaca agaaggccaa gagcggcatg tcggcggagc gaacccggat gaccctgaag
     4441 gagaccctgg gatgggccag agcgcagatc agaaggtgac ggagatccgg gcgacccgcg
     4501 cccaggtcga tcacgacctc cgggagctgg agcaccggct gccgatcccg ctgaacggac
     4561 ggaccatcgg gctgctgatg ggcgccggga cggcagcgag cctggtcctc aaggtcctgt
     4621 cctgggtgtg gaaacggcgg cgggagcagc gggcccggac cgccaacgtc gtggtccacg
     4681 tggtggacga gcggcacggc cggcatgacg agcgagacga cacggaggtc gcatgagtgg
     4741 ccagcccctc gatccgcgat cgatgagcgg ggacgggccg gatccccgaa ccgagccaga
     4801 tcttcggacg gcgatggagc cccggccggc gcaggatctc gggaacaccg tggaccaccc
     4861 cgccaagctc ctccggatcg cctccatggt ccgcgagctc caggaggaga tccgccgaga
     4921 gaccctggac gaggccggtg tggacaagct ccgggacgcc cagcaccgga tcctcgacga
     4981 gctgcaggac gtgctgtccg aggggctccg ggaggagctg tccgccctgg cggctcccgc
     5041 gggcgccggc gccaccgagt ccgagctccg ggtggtccag gcgcagttgc tcggctggct
     5101 ggagggcctg ttccagggca tccaggccgc cgtgttcacc cagcagatgc aggcccgggc
     5161 ccagctcgaa cagatgcggg gccggaacgc acccgcaccc tccggtggcc cgcagccacc
     5221 cgcgggcggc cagtacctct agcgctcgcc ctcacctctc cgactcgaac ggcgcgggac
     5281 ggcgatgccg catggcggag gctggcatgt gcgagaacct cggagcgccg gagacgctca
     5341 agccccgaca gatcttgtac gtcagatacc cgacgaccag cggcacgatc acggtgaccc
     5401 actgcagaaa cgccgtcacc gccccgatgc tcagctgcag ccagcgggcg tacaggtcgt
     5461 tgctggctga gaagaagagc atgatgtaga agctgagggt cgtcgtcccc accgccgtac
     5521 gcacggggcg atcccggggt cggtccagca ggttgtgcgg agcccggtcc ttggtgagcc
     5581 actgctcgag gaacggccac gcatacaaca ggccgaaggt cacccccggc aggaggacgg
     5641 cggggaagaa tgcgttcggc accaggaacc cgaagccctg gatcgtccag ttggggaaga
     5701 gcctgagggc gccctcgatc caccccaggt accagtcggg ctgggcgggc gaggtcacct
     5761 ggtaggggtg gaacggtcca tagagccaga tcggattgat ctgcgccagc ccacccaggg
     5821 cggcgacggc cgcgaacagg aggaagaaca gccccagcga cttggtggcg tacgtaggcc
     5881 acagacgcga gcccacgacg ttgtcctcgg tggtcagacc cggttcgggg aactgcgtgt
     5941 gcttctggtg ccagaccagt gccaggtgag ccccgatcaa gccggccagc agcgcgggca
     6001 cgatgaagat atgcagcacc agcaggcgac cgaggatttg gtcggagggg aactcgcctc
     6061 cgaacaccag gaacgccatc caggtcccga tcaccgggac gctctgcacg accgaataca
     6121 tgatccggag cccggtggcg gacagtaggt cgtccagcag cgagtaaccc gagaacccat
     6181 tgaacagggc cagcagcagc atggtgacgc cgatcagcca gttcagctcc cgggggcggc
     6241 ggaacgcccc ggtgaagaag atccggcaca ggtgggccac gatcgccgcg atgaacacgt
     6301 ccgccgtcca atggtgcgtc tgacgcatca ccaagccggc ccgcacgtcg aagctgagat
     6361 gcagcaccga ctggtaggcg gcggagacct ccagccctcg gagcggagcg taactgccct
     6421 ggtacacggt ctcggcggag ctggccttgt agaagaaggc caggaacgtg ccggtgagga
     6481 ccaggatgat gaagctgtag gccgcgatct ccccgagcat gaaggaccag tggtcgggga
     6541 agaccttgcc cagcgcgcgc tcggcg
//
LOCUS       DASQQQ010000031         2374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_30209, whole genome shotgun sequence.
ACCESSION   DASQQQ010000031 DASQQQ010000000
VERSION     DASQQQ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30209"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..312
                     /locus_tag="VE646_01715"
     CDS             55..312
                     /locus_tag="VE646_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78742.1"
                     /translation="MDDLVLIHTAPSTMAGLLVKGRLESEDIPVFAKGEIEGPYRFGP
                     IYLWVPRDVEVQARLIVAEMEAGGMALDAEDEAGAERTDRA"
     gene            complement(322..714)
                     /gene="rpsI"
                     /locus_tag="VE646_01720"
     CDS             complement(322..714)
                     /gene="rpsI"
                     /locus_tag="VE646_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HZD78743.1"
                     /translation="MAQETIRATGRRKEAVARVRITPGTGTFRLNDRTLEDYFPSRTQ
                     RMIVTAPLRAVEMEKQVDVVATIEGGGSNGQAGALSHGIARALIEMDGSLRGVLKKEG
                     YLTRDAREKERRKYGLKKARKAPQYSKR"
     gene            complement(715..1182)
                     /gene="rplM"
                     /locus_tag="VE646_01725"
     CDS             complement(715..1182)
                     /gene="rplM"
                     /locus_tag="VE646_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HZD78744.1"
                     /translation="MKTYTPKPSDIEHRWWLVDAEGATLGRLASEVASILRGKHKPTW
                     APHLDSGDHVVIVNAAKVRLSGAKREQKKWYRHSQFPGGLREIPYDRLLRERPDLAVE
                     KAVRGMLPKTRLGRRQIRKLHVYAGPEHSHQGQKPEPLELGRTPGAATTTEES"
     gene            complement(1179..1913)
                     /gene="truA"
                     /locus_tag="VE646_01730"
     CDS             complement(1179..1913)
                     /gene="truA"
                     /locus_tag="VE646_01730"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HZD78745.1"
                     /translation="MPVLKLVLAYDGTRFRGWARQPGQRTVQGTLERALGKMLGEAPR
                     LSVAGRTDAGVHARGQVVTLRTENDPLRIQRGLNGMLAPEVVVWRAGRAPEGFDARRS
                     ARAREYRYRVWTAEWPDPFTDRFMWHLPKALGLEPMRRAARSLEGEHDFASFCRAGSG
                     TTVRRLHRLAIRREGDLVEFRLRGNAFLHQMVRSLVGTLVDVGRGRKEAESMPEVLAA
                     RSRRFAGHPAPARGLTLERVLYRGRT"
     gene            complement(1953..2318)
                     /gene="rplQ"
                     /locus_tag="VE646_01735"
     CDS             complement(1953..2318)
                     /gene="rplQ"
                     /locus_tag="VE646_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HZD78746.1"
                     /translation="MPRPKKGPRLGAGPAHQKLMLGGLAADLFTHERVRTTAAKAKAL
                     RPVAERLITFAKRGDVHARRQVLRVVPDRDVVAHLFEDIAPRFADREGGYTRILKLGP
                     RKGDGAEMAQIELVELEEV"
BASE COUNT          295 a          907 c          806 g          366 t
ORIGIN      
        1 cccgcgcacg ccgtcggtgc cgaagagcct ggccatgagc ggtatggtac ccatatggac
       61 gatctcgtcc tcatccacac cgccccgtcg acgatggccg gcctgctcgt gaagggccgg
      121 ctggagagcg aggacatccc ggtcttcgcc aagggcgaga tcgaggggcc gtaccggttc
      181 gggcccatct acctctgggt gccgagggac gtggaggtcc aggcccggtt gatcgtcgcc
      241 gagatggagg cgggcgggat ggccctcgat gcggaggacg aggcgggcgc cgagcggacc
      301 gaccgggcgt agccgggcag cctaccgctt gctgtactgg ggcgccttcc gggccttctt
      361 cagcccgtac ttgcggcgct ccttctcccg cgcatccctc gtgaggtagc cctccttctt
      421 caggacgccc cggagggacc cgtccatctc gatcagggcg cgggcgatcc cgtgtgagag
      481 tgcgccggcc tggccgttcg accccccacc ctcgatcgtg gcgacgacgt cgacctgctt
      541 ctccatctcg acggcgcgga ggggcgcggt cacgatcatc cgctgggtcc gggaggggaa
      601 gtagtcctcg agcgtccggt cgttgagccg gaacgtgccg gtgccgggcg tgatgcgcac
      661 gcgggccacg gcctccttcc ggcggccggt ggcgcggatc gtctcctggg ccatctagga
      721 ctcctccgtg gtggtcgcgg cgcccggcgt ccgaccgagc tcgaggggct cgggcttctg
      781 accctggtgg ctgtgctcgg ggcccgcgta gacgtgcagc ttcctgatct ggcggcgccc
      841 cagcctcgtc ttgggcagca tgccgcggac ggccttctcg acggcgagat cgggccgctc
      901 ccggaggagg cgatcgtagg ggatctcgcg gagcccgccc gggaactggg agtggcggta
      961 ccacttcttc tgctcgcgct tggcgcccga cagccgcacc ttcgcggcgt tcacgatcac
     1021 gacgtggtcg ccggagtcca ggtggggcgc ccacgtgggc ttgtgcttgc ccctgaggat
     1081 gctcgccacc tcgctcgcca gccggcccag cgtggcaccc tcggcgtcga cgagccacca
     1141 gcggtgctcg atgtcgctcg gcttcggtgt gtaggtcttc atgtccgtcc tcgatagagt
     1201 acccgttcga gggtcagccc acgggccggg gcaggatggc ccgcgaaccg tctggatcgc
     1261 gcggccagca cctccggcat ggactccgcc tccttccgtc ctcgtcccac atcgaccagc
     1321 gtccccacca ggctgcgcac catctggtgg aggaaggcgt tcccgcggag ccggaactcc
     1381 acgaggtcgc cctcccggcg gatcgccagc cggtgcagcc ggcgaaccgt cgtccccgag
     1441 cccgcccggc agaacgaggc gaagtcgtgc tcgccctcga gcgaccgggc cgcccggcgc
     1501 atcggctcca ggcccagggc cttcggcaga tgccacatga agcggtccgt gaacgggtcc
     1561 ggccactcgg cggtccagac ccggtaccgg tattcccggg cccgggcgct ccgccgggcg
     1621 tcgaacccct ccggggcccg ccccgcccgc cacaccacca cctcgggggc cagcatgccg
     1681 ttcagccccc gctggatccg cagcgggtcg ttctcggtcc gcagcgtgac gacctggccg
     1741 cgagcgtgca cgcccgcgtc ggtgcgtccc gccaccgaca gccggggagc ctcccccagc
     1801 atcttcccca gcgcccgctc cagggtcccc tggaccgtcc gctggccggg ctggcgcgcc
     1861 cagccccgga acctcgtccc gtcgtaggcg agcacgagct tcaggaccgg catccgcccg
     1921 gacaccctgc cacccggagt cgcccccggg ccctacacct cctcgagctc gacgagctcg
     1981 atctgcgcca tctccgcccc gtcgcccttc cggggcccca gcttcaggat ccgcgtgtag
     2041 ccgccctcgc gatccgcgaa ccggggcgcg atgtcctcga acaggtgcgc gacgacatcg
     2101 cggtcgggca cgaccctgag gacctgccgc cgcgcgtgca cgtcgccccg cttcgcgaag
     2161 gtgatgagcc gctcggccac cgggcgcagc gccttggcct tcgccgccgt ggtccgcacc
     2221 cgctcgtgcg tgaacaggtc ggcggccagc ccgccgagca tcagcttctg gtgggccggt
     2281 cccgcaccga ggcgcggacc cttcttcggc ctcggcacgg tcactcctcc ttcagcgaca
     2341 gcccgagctc ctcgagcttg cccttgacct cgtc
//
LOCUS       DASQQQ010000032         2486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_222081, whole genome shotgun sequence.
ACCESSION   DASQQQ010000032 DASQQQ010000000
VERSION     DASQQQ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222081"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VE646_01740"
     CDS             <1..345
                     /locus_tag="VE646_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006374301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HZD78747.1"
                     /translation="SVHDRVRKLEQTGVIRSYRAVVNPEAVGLFVTALIAVTPLDPTQ
                     PDDLPERVQEFREVIDCYSVAGEANYILKVRTGTTTDLEDLIRRLREKGEVQTRTTVV
                     LSVPFEDRPLRP"
     gene            412..1806
                     /gene="cysS"
                     /locus_tag="VE646_01745"
     CDS             412..1806
                     /gene="cysS"
                     /locus_tag="VE646_01745"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HZD78748.1"
                     /translation="MRLYDSLTRELKEVVPVDGETVKIYSCGPTVYRYIHIGNMRSFM
                     LGDLIRRALRFEGHEVRWIMNITDVGHMTDEVSDTGRDKMELAEADEGSSAAQIAEKY
                     TQAFLEDADLVGIERADLYPRATEHIEDMIRIIQVLLDRGHAYQAGGTVYYDVTTFPE
                     YGKLSGNTLDQLRAGHRQELATDPNKRHPEDFALWKQAGPNRLLKWPSPWGEGFPGWH
                     IECSAMSMKHLGERFDIHTGGNDNKFPHHEDEIAQSEGAVGHAVVSVWVHGGFLQMGG
                     QKMAKSARNIKRVTDLAEQGIDPLAYRLLCFGTRYRSEMDFSWEALEGQHARLRDLRR
                     RMLAWSSAPRPGAPSTAGAELDRRFRDALSDDLNLPHALVAVNDAVGSVELEPGERYA
                     LLSSWDHVLGLDLERLVREQWEPTAEMLELVRRRDEARAAKDWTTADELRERLQSMGL
                     EVMDTPEGTKIRPA"
     gene            complement(1872..>2486)
                     /locus_tag="VE646_01750"
     CDS             complement(1872..>2486)
                     /locus_tag="VE646_01750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014441932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZD78749.1"
                     /translation="VRTHRFAGRREAEVAADLAQALREHGHESVDFTIVGSGPNGASP
                     HHEAGDRVIQAGEAVVMDFGGFAAGYGSDMTRTVVVREPPEGLEEVYGLVRRAQQAAF
                     ETVRPGAACEDVDRVARAVIVDAGYGEAFFHRTGHGIGLETHEEPYIVAGNRTALEPG
                     MTFSIEPGIYLPGRFGVRIEDIVAVTEDGATRLNEASRDLQVVA"
BASE COUNT          388 a          888 c          868 g          342 t
ORIGIN      
        1 tcggtccacg accgcgtccg caagctggag cagaccggcg tgatccgctc ctaccgcgcg
       61 gtggtcaacc ccgaagcggt gggcctgttc gtcacggccc tgatcgcggt cacgccgctg
      121 gaccccaccc agcccgacga cctgcccgag cgcgtgcagg agttccgaga ggtgatcgac
      181 tgctacagcg tggccggcga agccaactac atcctcaagg tccgcaccgg caccaccacc
      241 gatctggagg atctgatccg gcggctgcgg gagaagggcg aggtgcagac ccgcaccacg
      301 gtggtcctgt ccgttccttt cgaagaccgc cccctgcgcc cctgaacctc gtccgtccga
      361 gaggtggacc ggccggccgt gccgccgcgg gccacgggta tgcttgcctg catgcgcctg
      421 tacgactcgc tgacgcgtga gctgaaggag gtcgtcccgg tcgatgggga gaccgtgaag
      481 atctactcgt gcgggccgac cgtctaccgg tacatccaca tcgggaacat gcgctcgttc
      541 atgctggggg acctgatccg ccgagcgctg cggttcgagg gccacgaggt gcggtggatc
      601 atgaacatca ccgacgtcgg ccacatgacg gacgaggtct cggacaccgg ccgggacaag
      661 atggagctgg ccgaggccga cgagggatcg agcgcggccc agatcgccga gaagtacacg
      721 caggccttcc tggaggatgc ggacctcgtg ggcatcgagc gcgccgacct gtacccgcgc
      781 gccaccgagc acatcgagga catgatccgg atcatccagg tcctgctgga tcgggggcac
      841 gcctaccagg ccggcggcac cgtctactac gacgtgacca cgttccccga gtacgggaag
      901 ctctcgggca acaccctgga ccagctgcgc gccggccatc ggcaggagtt ggccaccgac
      961 cccaacaagc gccatccgga ggacttcgcg ctgtggaagc aggccgggcc gaaccgcctg
     1021 ctgaagtggc cgagcccctg gggcgagggc ttcccgggct ggcacatcga gtgctcggcg
     1081 atgtcgatga agcacctcgg ggagcgcttc gacatccaca ccggaggcaa cgacaacaag
     1141 ttcccccacc acgaggacga gatcgctcag tccgagggcg ccgtgggcca tgcggtggtc
     1201 tcggtgtggg tgcacggagg gttcctgcag atgggggggc agaagatggc caagtccgca
     1261 cggaacatca agcgggtcac ggacctggcc gagcagggca tcgatcccct cgcctaccgc
     1321 ctgctgtgct tcgggacccg gtaccgcagc gagatggact tcagttggga ggccctggag
     1381 ggtcagcacg caaggctgcg ggacctccgg cgacggatgc tggcgtggtc atcggccccc
     1441 cggccgggcg cgccctcgac ggccggcgcc gagctggacc gccggttccg agacgccctg
     1501 tccgacgacc tgaacctgcc gcatgcgctg gtggcggtga acgatgcggt cggctcggtg
     1561 gagctcgagc ccggtgagcg gtacgccctg ctgtcctcgt gggaccacgt gctggggctg
     1621 gacctggagc gcctggtccg cgagcagtgg gagccgacgg ccgagatgct ggagctggtc
     1681 cgacgccgcg acgaggccag ggccgccaag gactggacca cggcggacga gctccgggag
     1741 cgcctccagt cgatggggct cgaggtcatg gacacgcccg aggggaccaa gatccgcccg
     1801 gcctgagccg ggtcccgggc gcctggcccg ccccggggtc cggccctcga ggtcacccgg
     1861 gcgccgggtg ctcaggccac cacctgcagg tcccggctcg cctcgttcag ccgggtcgct
     1921 ccgtcctcgg tcacggccac gatgtcctcg atccgcaccc cgaaccgacc gggcaggtag
     1981 atcccgggct cgatcgagaa cgtcatgccg ggctccaggg ccgtccggtt gccggccacg
     2041 atgtagggct cctcgtgcgt ctccaggccg atgccgtggc cggtgcggtg gaagaaggcc
     2101 tccccgtagc cggcgtcgac gatcacggcc cgcgccaccc ggtccacgtc ctcgcaggcc
     2161 gcccccgggc gcaccgtctc gaacgccgcc tgctgcgccc gccgcacgag cccgtagacc
     2221 tcctcgagcc cctccggagg ctcgcgcacg accacggtgc gcgtcatgtc cgagccgtag
     2281 ccggccgcga accccccgaa gtccatcacc acggcctcgc cggcctggat cacccggtcc
     2341 cccgcctcgt ggtggggaga ggcgccgttc ggaccggagc ccacgatcgt gaagtcgacg
     2401 ctctcgtggc cgtgctcgcg cagggcttgg gccaggtcgg ccgccacctc ggcctcccgc
     2461 cgcccggcga accgatgggt ccgaac
//
LOCUS       DASQQQ010000033         7595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_195995, whole genome shotgun sequence.
ACCESSION   DASQQQ010000033 DASQQQ010000000
VERSION     DASQQQ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195995"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VE646_01755"
     CDS             complement(<1..378)
                     /locus_tag="VE646_01755"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZD78750.1"
                     /translation="MAHPRHVLVTGGAGFIGSHLVDLLLREGDHRVSVLDRLSQGGRR
                     ENLDQHQDDPRFTFVLGDVNDGELVDRLVSGADAIVHCAAQAHVDRSIVGPRDFVLTN
                     LLGTQAVLDACRARGRRLLMVSTD"
     gene            complement(391..1548)
                     /locus_tag="VE646_01760"
     CDS             complement(391..1548)
                     /locus_tag="VE646_01760"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZD78751.1"
                     /translation="MRLAIDVRAALDPRRTGVGHYAQQLVRHIPHADPDDEFVAWYLH
                     ARGVFRSRRFFADGGAPNLTEDAARFPARVFEPLSARLGIPRLEWFLDFDALLATNFL
                     PPATRSRSVVMVVHDLAFRVFPDTAPQIDERWLRRFRGWLSNAARVIVPSHAAQDDLC
                     AMEEVDPDKIVVIPHGVDAEGIRSAGAKRVEAARKRLGLDRPYLLFVGGIEPRKNLPA
                     LVHAFARLDEETTLVIAGGKVRWFPDAADRLREVVEALPEPVRRRVILAGYVSEGDKL
                     GLLAGARGVVYPSLYEGFGFPVLEGMAARVPVLTSNVSSLPEVAGDAAILVDPHDEGA
                     IVRGLDELLHDEDLRERLIAAGLARAARFTWEDTSRRTAGVLREVASEGGG"
     gene            complement(1582..2637)
                     /locus_tag="VE646_01765"
     CDS             complement(1582..2637)
                     /locus_tag="VE646_01765"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HZD78752.1"
                     /translation="MRALVLSGGEGSRLRPITHTGAKQLIPIANTPILFHGLRQIREA
                     GITEVGVVVGPPNEQAVRAAVGDGSAWGLRVTFIQQAAPLGIAHAVSTAADFVRGEPF
                     LLYLGDNVLLEGVSRFVREFERSRPDAQILLAHVPEPEHFGVAVLEGERVVRLVEKPR
                     EWISDVALVGVYLFDDSILEACATLRPSWRDEYEITEAIQWLIDRGRTVRAELVSGWW
                     KDTGRPEDLLEANRMMLSVLEPSGEGEVDGASSVEGVVVIGPGAKVVRSTLRGPVILG
                     EGTLVEDSTVGPNASVSFGCRVVGSTVEDSIVMERTRIEGVRGISSSIFGRDVELRGE
                     RGPRRFVIGDQSHIEID"
     gene            complement(2634..4052)
                     /locus_tag="VE646_01770"
     CDS             complement(2634..4052)
                     /locus_tag="VE646_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HZD78753.1"
                     /translation="MTDPTSPDRRWHEHPHLRRAFLAGAASIAVLVTVLSSTAFGLYW
                     YTAKKIHTIGPESPAPSGASPTPAPITGRCAKTACNYLLLGSDSRTGLSKEEQTAFGT
                     NKDIGGSQRSDTIIVVHVEPDQKKAVFLSFPRDLWVDIPGHGQNKINAAFEGGIEHGG
                     AQLVARTVTALTGLEIDHVMYVDLAGFQGVVDALGGVVMCVPYPMQDPLTALDISAGC
                     QHFNGQTALAYVRTRHQPCDTIPDFARISRQQQFLRAVIAKLLSPREIFRLPSLVGPI
                     LDSIRVDQGLNPAELTYLAGQLNGVNTGNVDFRVVPTVPTGTYVNGQYLSIVKLVQPQ
                     ADQLFERLRKGRALGKLGIAQDQTAPSPAVIQVGVFDKRSQGAAVSVHSTLTQGGFDT
                     STPVQDVSVLVRLPAKGSVILFNPNAPDGQAMADVVHGYLSNLDEQAAPKHLLQGLDV
                     AVIVGPNYTVPPPNTSGPVNCP"
     gene            4176..5060
                     /locus_tag="VE646_01775"
     CDS             4176..5060
                     /locus_tag="VE646_01775"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZD78754.1"
                     /translation="MAATSGTPAMGATVLIVDGDGHRAERALGALRSDTAVAVRPLIA
                     SDPDQISERLGQDAVDVALVAFEPSAPELLEVMRERAPEVPVIAVVADPEAASEALDA
                     GAQDFVAERDLDGEVLRRTVRYALDRRQLREELDRRTFIDELTGLYNINGLEQIAAHH
                     LRLADRTHQPLVLVFVRIEELASISQTYGAAEGPRLLADTARILRSAVRSSDIIARVG
                     QDAFCALLTANATGAETLVLARMVEAVATHNARGGRPYPMALSVGAAAYDPDHPAGLS
                     QLIREADARQRERRAPIR"
     gene            complement(5065..5472)
                     /locus_tag="VE646_01780"
     CDS             complement(5065..5472)
                     /locus_tag="VE646_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZD78755.1"
                     /translation="MFEKILLAVDGSEPSQRAVEVAAQEAIAHRAEVLVLHVCEQELT
                     WAVEVEAPSEAAGLIDGVVADLKDRGVSARGDVLRAPLGQTPRVILNVARDESADLIV
                     MGTRGLSDWGRLLMGSVAHKVVHLATCPVLVVR"
     gene            5686..6018
                     /locus_tag="VE646_01785"
     CDS             5686..6018
                     /locus_tag="VE646_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78756.1"
                     /translation="MNKRSAMIVAGGLVGAVASTAGAVSAGLASGPTAQAQTEPVRAT
                     KPVVKTIHRTITIHREPKHQAAPVRAIAVGPSAAASTAPLTHSSGSLAAGGFDDEHEG
                     GGFEGGDD"
     gene            6011..6328
                     /locus_tag="VE646_01790"
     CDS             6011..6328
                     /locus_tag="VE646_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78757.1"
                     /translation="MTDRPRRRRRLPKRVLRALAWVTGGVAFFSPWTVLGLSPKPAAG
                     EATKTARASRQVIVVKKITRKVIIQDVVTAAPVSSVSSTSSGGSAAIAAPVTTTSGST
                     PPP"
     gene            6370..7224
                     /locus_tag="VE646_01795"
     CDS             6370..7224
                     /locus_tag="VE646_01795"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZD78758.1"
                     /translation="MSLIAAGDADAGSSHRAAGVVEHLFSREELRFSRFRGDSELSRV
                     NARAGTWTRVSPGFAALVAYALEAAAGSAGLFDPTVLPALIAAGYDRDFDELIAGARD
                     ALHPSEPCGRWPEIELEGNLLRLPPGVGLDLGGIAKGWTVDLAAQAAVDQGLDWAIVN
                     AGGDLRIAGTPPAEAFPIGIDDPEVPEQELTRIRVASGALATSSITARAWGPGRHHLI
                     DPRTGLPADTEVVQATVWAETCAEAEIGSKQALLTGVPILDRLTGVLVTRDGRIVMNI
                     PSEEEARA"
     gene            7221..>7595
                     /locus_tag="VE646_01800"
     CDS             7221..>7595
                     /locus_tag="VE646_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78759.1"
                     /translation="MTTWIILRAAGIGAYLMLFLSVSWGLVATTGALGKRVSKASATL
                     VHQFMATTGLVLLGIHLGGLSIDAFVPFGPADLLIPLHGTFKPVAVALGIVAMYLSVA
                     VVVLSWLRKRIGTTWWRRSHELA"
BASE COUNT         1091 a         2767 c         2698 g         1039 t
ORIGIN      
        1 gtccgtggag accatcagca gcctccgtcc gcgagcccgg cacgcatcca gcaccgcctg
       61 ggtccccagc aggttcgtga gcacgaagtc ccgaggcccg acgatgctcc ggtccacgtg
      121 ggcctgggcg gcgcagtgca cgatggcatc ggcccccgac accagccggt cgaccagctc
      181 cccgtcgttc acatcgccca gcacgaaggt gaaccgagga tcgtcctggt gctgatcgag
      241 gttctcgcgc cgacccccct gcgagagccg gtcgaggacg ctcacacggt ggtcgccctc
      301 gcgcaggagc aggtcgacca ggtgggagcc gatgaacccg gcacccccgg tgaccagcac
      361 atgtctcggg tgggccatgg ccgcccgagt ctaccccccg ccctcggagg cgacctcccg
      421 caggaccccc gccgtccggc gggacgtgtc ctcccaggtg aagcgcgccg cccgggccag
      481 gccggccgcg atcaggcgct cacgcaggtc ctcgtcgtgg agcaactcgt ccaggcctcg
      541 gacgatggcc ccctcgtcgt gggggtccac caggatcgcg gcgtcgcccg ccacctccgg
      601 cagggacgag acgttcgagg tcaggaccgg cacccgcgcg gccatcccct ccagcacggg
      661 gaacccgaag ccctcgtaca gggagggata gaccactccc cgggcgccag cgagcagccc
      721 gagcttgtcc ccctcgctca cgtagcccgc caggatcacg cgccgccgca ccggctcggg
      781 cagcgcctcc accacctcgc gcagccggtc ggcggcgtcc gggaaccacc ggaccttccc
      841 tccggcgatc acgagcgtcg tctcctcatc caggcgggcg aaggcgtgga ccagcgccgg
      901 caggttcttg cggggctcga tgccgcccac gaagagcagg taggggcggt ccaggccgag
      961 ccgcttgcga gccgcctcca ctcgcttggc cccggccgag cggatcccct cggcgtcgac
     1021 cccgtgcggg atcaccacga tcttgtccgg atccacctcc tccatcgcgc acagatcgtc
     1081 ctgggcggcg tggctgggca cgatcacccg ggcggcgttc gacagccagc cccggaaccg
     1141 gcgcagccac cgctcgtcga tctgcggcgc ggtgtcgggg aacacccgga aggccaggtc
     1201 gtgcaccacc atgaccaccg agcggctgcg ggtggccggc ggcaggaagt tcgtggccag
     1261 gagcgcgtcg aagtccagga accactccag gcgagggatg cccaggcggg ccgacagggg
     1321 ctcgaagacc cgggccggga accgggcggc gtcctcggtg aggttcggag cccccccgtc
     1381 cgcgaagaac cggcgggagc ggaacacccc tcgggcatgc aggtaccagg ccacgaactc
     1441 gtcgtccggg tcggcgtgcg ggatgtgccg gaccagctgc tgggcgtagt gccccacccc
     1501 ggtccggcgc gggtccagcg ccgctcgcac gtcgatggcc agtcgcaccg gccgaagcgt
     1561 agcccgggtg ggaggccggt cctagtcgat ctcgatgtgg ctctggtcgc cgatcacgaa
     1621 gcgacggggg ccgcgctcgc cccggagctc gacgtcccgg ccgaagatgg aggagctgat
     1681 cccgcgcaca ccctcgatcc gggtgcgctc catcacgatc gagtcctcca ccgtgctgcc
     1741 caccacccgg cagccgaagg agaccgaggc gttgggcccc acggtcgagt cctccaccag
     1801 cgtgccctcg cccaggatca ccgggccgcg gagcgtggag cgcaccacct tggcacccgg
     1861 gccgatcacg accacgccct ccacgctcga ggctccgtcg acctcgccct cccccgaggg
     1921 ctccagcacc gagagcatca tgcggttggc ctcgagcagg tcctcggggc ggccggtgtc
     1981 cttccaccag cccgacacga gctcggcccg tacggtccgt ccccggtcga tcagccactg
     2041 gatcgcctcg gtgatctcgt actcgtcgcg ccaggagggg cggagcgtgg cgcaggcctc
     2101 gaggatcgag tcgtcgaaca ggtagacgcc gaccagggcc acgtcggaga tccactcgcg
     2161 gggcttctct accaagcgaa ccacccgttc cccctccagc accgccacgc cgaagtgttc
     2221 gggctccggg acgtgggcca gcaggatctg ggcgtcgggc cgggagcgct cgaactcccg
     2281 cacgaagcgg ctcaccccct ccagcaggac gttgtcgccc aggtacagca agaagggctc
     2341 gccgcgcacg aagtcggcgg cggtggagac ggcgtgcgcg atgccgaggg gcgccgcctg
     2401 ctggatgaag gtcacccgca gtccccaggc cgatccgtcg cccacggcgg cccgcaccgc
     2461 ctgctcgttc gggggaccca ccaccacccc gacctcggtg atcccggcct cgcggatctg
     2521 ccgcaggccg tggaacagga tcggcgtgtt ggcgatgggg atcagctgct tggcgccggt
     2581 atgggtgatg ggccgcaggc gcgacccctc gccccccgag agcaccagcg ccctcacggg
     2641 cagttcaccg gtccggacgt gttgggggga gggaccgtgt agttcgggcc cacgatgacc
     2701 gccacgtcca gtccctgcag caggtgcttg ggcgcagctt gctcgtccag gttcgagagg
     2761 tacccgtgca ccacgtcggc catggcctgg ccgtccggcg cgttcgggtt gaacaggatc
     2821 accgagccct tggcggggag ccgaaccaag acgctcacgt cctgcaccgg agtggaggtg
     2881 tcgaagcctc cctgggtcag ggtcgagtgc acgctcacgg ccgccccttg cgagcgcttg
     2941 tcgaacacgc cgacctggat cacggccggg gagggagccg tctggtcctg ggcgatcccc
     3001 agcttgccca gcgctcgtcc cttgcgcagg cgctcgaaca gctggtcggc ctggggctgc
     3061 accagcttga cgatcgacag gtactggccg ttcacgtagg tgcccgtcgg gaccgtcggg
     3121 actacacgaa agtccacgtt gcccgtgttc acgccgttca gctgcccggc caggtaggtg
     3181 agctcggcgg ggttcaggcc ctggtccacc cggatcgagt ccaggatcgg gccgaccagg
     3241 gagggcaggc ggaagatctc gcgcggcgag agcagcttgg cgatcacggc ccggaggaac
     3301 tgctgctggc ggctgatccg ggcgaagtcg gggatcgtgt cgcagggctg gtgccgggtc
     3361 cgcacgtacg ccagggcggt ctgaccgttg aagtgctggc agccggccga gatgtccagg
     3421 gccgtgaggg ggtcctgcat cgggtacggc acgcacatca cgaccccgcc cagggcatcc
     3481 accaccccct ggaacccggc caggtccacg tacatcacgt ggtcgatctc gagtccggtg
     3541 agggcggtga cggtccgggc caccagctgc gcgccaccgt gctcgatccc cccctcgaag
     3601 gcggcgttga tcttgttctg gccgtgcccg gggatgtcca cccacaggtc ccgcgggaac
     3661 gacaggaaca ccgccttctt ctggtccggc tcgacgtgca ccacgatgat cgtgtccgag
     3721 cgttgggagc cgccgatgtc cttgttcgtg ccgaaggccg tctgctcctc cttcgagagc
     3781 ccggtacggc tgtcgctgcc caggagcagg tagttgcagg ccgtcttcgc gcagcggccc
     3841 gtgatgggcg cgggcgtcgg tgaggcaccg gagggagccg gcgattccgg cccgatcgtg
     3901 tggatcttct tggccgtgta ccagtagagc ccgaacgcgg tcgacgacag gacggtgacg
     3961 aggacggcga tcgaggccgc cccggccagg aaggcccggc gcaggtgcgg atgctcgtgc
     4021 cagcggcgat cgggggacgt cggatccgtc atggctccgg agaagcctac ccgcaggtgg
     4081 acccgggggc cggaccggcc cgtggcgttc ctgtggagcc ggcggccggc tccgagggct
     4141 gggacccggc accccctcac gcataagcta cggccgtggc tgcgacgtcc ggaacgccgg
     4201 cgatgggggc gacggtgctg atcgtcgatg gcgacggcca tcgagccgag cgcgccctgg
     4261 gggccctgcg ctcggacacg gcggtggccg tgcgtccgct gatcgcctcc gacccggacc
     4321 agatatcgga gcgtctgggg caggacgccg tggacgtggc gctcgtggcc ttcgagccct
     4381 cggcgcccga gctgctggag gtgatgcggg agcgggcgcc ggaggtcccg gtgatcgccg
     4441 tggtggccga ccccgaggcg gccagcgagg ccctggacgc cggcgctcag gacttcgtgg
     4501 ccgagcgcga cctggacggg gaggtgcttc ggcggaccgt gcgctacgcg ctggaccgcc
     4561 ggcagctccg ggaggagctc gaccggcgca cgttcatcga cgagctcacc ggcctgtaca
     4621 acatcaacgg cctcgagcag atcgccgctc accacctccg cctggccgac cgcacccacc
     4681 agccgttggt gctggtgttc gtccggatcg aagagctggc ctcgatcagc cagacgtacg
     4741 gtgccgccga gggcccccgc ctgctggcgg acaccgcccg gatcctgcgc tcggccgtcc
     4801 gcagctcgga catcatcgcc cgggtgggcc aggacgcctt ctgcgcgctg ctcaccgcga
     4861 acgccacggg agccgagacg ctcgtgctgg ccaggatggt ggaggccgtg gccacgcaca
     4921 acgcccgggg gggacggccc tacccgatgg ccctgtccgt gggggccgcc gcgtacgacc
     4981 cggaccatcc ggccggcctc tcgcagctga tcagggaagc cgacgcccgc caacgcgagc
     5041 gtcgagcccc gatccgctag tcgctcagcg gacgaccagc accggacagg tggccaggtg
     5101 gaccaccttg tgggccacgc tgcccatcag gagccggccc cagtcggaca ggccgcgcgt
     5161 gcccatcacg atcaggtcgg cgctctcgtc gcgcgccacg ttgaggatca cccgaggggt
     5221 ctgcccgagc ggcgcgcgga ggacgtcgcc ccgagcgctc acgccccgat ccttcaggtc
     5281 cgccaccacg ccgtcgatca gcccggccgc ctccgagggc gcctccacct ccacggccca
     5341 ggtcagctcc tgctcgcaca cgtgcaggac caggacctcg gcgcggtggg cgatcgcctc
     5401 ctgggctgcc acctcgacgg cccgctggct gggctcggat ccgtcgaccg caaggaggat
     5461 cttctcgaac atctgcgcct ccttcgtccc accgccagct tggcgaaccg ggtggttcgg
     5521 ggagagggcc ggatggcccc ggtcggagcc ggccgaacgg cccggaccgc acatccgagg
     5581 gtttagcgag cccttaaccc tccattgacg ctccgatggg agccgccctc ctagcgtggt
     5641 cgccgtgccc gtttcgccac gtccacgggc tcgaggaggg atcacatgaa caagcgctcc
     5701 gcgatgatcg tcgccggcgg gctggtcggc gcggtggcct ccacggccgg cgccgtctcg
     5761 gccggcctcg ccagcggtcc caccgcccag gcccagaccg agccggtccg agccaccaaa
     5821 cccgtggtca agacgatcca ccggacgatc acgatccacc gcgaacccaa gcatcaggcg
     5881 gcaccggtgc gggcgatcgc cgtggggccc tcggccgccg cgagcacggc ccccctcacc
     5941 cactcgtccg gctcgctggc ggccggcggg ttcgacgacg aacacgaggg cggcgggttc
     6001 gagggcggcg atgactgacc gtccccgccg acgtcgccga ctgcccaagc gcgtgctgcg
     6061 agcgctggcg tgggtcaccg gtggcgtggc gttcttctcc ccctggacgg tcctggggct
     6121 ctcccccaag ccggccgccg gcgaggccac gaagaccgcc cgggcatcac ggcaggtgat
     6181 cgtggtcaag aagatcaccc gcaaggtcat catccaggac gtggtcacgg cggcgccggt
     6241 cagctccgtg tcgtccacct cgagcggggg atccgccgcg atcgcggcgc cggtgaccac
     6301 cacgagcggc tccacccccc cgccatgagc gatccacgcg gtcaccggtt ccgggcgatg
     6361 ggcacgagcg tgagcctgat cgctgcgggg gacgccgatg ccgggagctc ccaccgggcg
     6421 gcgggggtgg tggagcacct cttctcccgc gaggagctcc gcttctcccg attccggggc
     6481 gacagcgagc tcagccgcgt gaacgcgcgc gccggaacat ggacccgggt ctccccgggg
     6541 ttcgccgccc tcgtcgccta cgcgctggag gccgcggccg ggtcggccgg cctgttcgac
     6601 ccgaccgtgc tgccggcgct gatcgccgcg ggctacgacc gcgacttcga cgagttgatc
     6661 gcgggtgccc gcgacgcgct gcaccccagc gagccctgcg gacggtggcc cgagatcgaa
     6721 ctggagggga acctgctcag gttgcctccc ggggtggggc tcgacctcgg cgggatcgcc
     6781 aagggctgga cggtggacct ggccgcccag gccgcggtgg accagggcct ggactgggcc
     6841 atcgtgaacg ccggcggcga cctgcggatc gcggggacgc ctcccgcgga ggccttcccc
     6901 atcgggatcg acgacccgga ggtccccgag caggaactga cacggatccg cgtggcatcg
     6961 ggcgccctgg ccacctcgtc gatcacggcc cgggcctggg gaccggggcg gcaccacctg
     7021 atcgaccctc ggaccggtct gcccgcggac acggaggtgg tccaggcaac ggtgtgggcc
     7081 gagacctgtg ccgaggccga gatcggctcc aagcaggccc tgctgaccgg cgtgccgatc
     7141 ctcgatcggc tgaccggggt gctggtgacc cgggacggcc ggatcgtgat gaacatcccg
     7201 agcgaggagg aggcgcgggc gtgacgacct ggatcatcct tcgggccgcc gggatcggcg
     7261 cctacctgat gctgttcctg tcggtgagct ggggactcgt ggccaccacc ggcgccctgg
     7321 gcaagcgggt ctccaaggcc tcggccacgc tggtccacca gttcatggcc accaccggac
     7381 tggtgctcct ggggatccac ctggggggcc tgtcgatcga cgccttcgtg cccttcgggc
     7441 cggcggacct gctgatcccg ctgcacggga ccttcaagcc ggtggcggtg gccctcggga
     7501 tcgtggcgat gtacctgagc gtggccgtgg tggtcctgtc gtggctccgc aagcggatcg
     7561 gcacgacctg gtggcggcgc tcgcacgagc tggcc
//
LOCUS       DASQQQ010000034         4372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_1249, whole genome shotgun sequence.
ACCESSION   DASQQQ010000034 DASQQQ010000000
VERSION     DASQQQ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VE646_01805"
     CDS             complement(<1..570)
                     /locus_tag="VE646_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZD78760.1"
                     /translation="MTVAVITDSAAALPDEVAEEAGVVVVPMWVTVGADTLRDTELPL
                     PELLRRADEGITTSAPSPGDLKEAIEARLGPEGALVVTVASRMSGSYQAARMAAGSFG
                     DAVGVLDSGTAAGAQGLVALAAAGCARLGGSLDDVRARAERVAARVRLVASLGDLDWL
                     VRGGHVPEVVAWAGRSLGLRPVIEFKGGRA"
     gene            complement(567..1877)
                     /locus_tag="VE646_01810"
     CDS             complement(567..1877)
                     /locus_tag="VE646_01810"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD78761.1"
                     /translation="MNIPLGHRGFRNLFVGQAVSALGDWMGTVALIVLVLDLTNSSTA
                     VGGVLVLQLLPSAVGGPVASRVVGRWRRRRTMMGADLFRVAVAVVLPLVQALWWVYLW
                     AFFLQLGGLVFLPARDASIPDLVEGDEDQLSLANGIILGSSFGTIPLGAAAFGGVAGL
                     QAMLFGHSGPIGSRSYLLVFLIDALTFLISFAFVRSLHVLDERGEQPEQEQGSEERTK
                     FLKAFRIPLVRMVVPAGATITLGIGALFSVGVKFVRQVLEASDTQFGILIALFGVGAA
                     AGLGTLQALRHRDQLIMVRTAVALQGIVVAVMSLSPNIGIAYIGAGFFGASSAASLVA
                     GMNVLQTRLEGGDRVQAFTAFHVTIRAGLAVAALVAGVAVDLVNGVRWPFLGQLQPSR
                     VVLLSAGALVFLSAAWVHEPAAEGERGPRGSERPGGRSLEEPDR"
     gene            complement(1885..2505)
                     /locus_tag="VE646_01815"
     CDS             complement(1885..2505)
                     /locus_tag="VE646_01815"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HZD78762.1"
                     /translation="MRRLPAPAVLALSYLCGAIPFSNLVAHRLRHVDLRSVGTGTVSG
                     SGLYRIAGFGPLALAGTLDVAKGAVGPLLAGRERPLIAGLAGGAAVAGHDWSPFLRGA
                     GGRGLSTALGALLVNGWEGVVVLSVGLAGGKLAKRTSIGCFIAYLALVPVLRATRGPS
                     GMVAGGSVVVPLLVKRVLGNRPAVGARRWRIYLNRLVLDQDQPRRR"
     gene            2665..3597
                     /locus_tag="VE646_01820"
     CDS             2665..3597
                     /locus_tag="VE646_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019810376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD78763.1"
                     /translation="MRGIRRSLRMDTAIRTEALTKSYGRARGVIDLGFEVPRGEVFGY
                     LGPNGAGKTTTIRTLLDLIRPTSGRAEVLGMDSHEDSLEIHRRTGYLPGELALYDRMT
                     GSDYLQHLAALRGGVEWAYVRDLAGRLDCDLGARIRSLSHGNRRKVALIQAFMHRPDL
                     LVLDEPTQGLDPLVQREFHHLVTQVRDRGCTVFLSSHVMPEVERLCDRVGIIREGRLI
                     AVEDIGDLKARSIRTLDVHFAHPVDPLHFAGLPGVKEATQHGDAVRITVAGPLDAVVK
                     AVATFPVVELTSHEPSLEEVFLTFYGKREDDDGA"
     gene            3587..>4372
                     /locus_tag="VE646_01825"
     CDS             3587..>4372
                     /locus_tag="VE646_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZD78764.1"
                     /translation="MARDLALRPHGVWLGTVFARTLREQRRALLWWTLGVVGTVLMYA
                     AIWPTIRQNAVDLNRYLDSFPDAMRNLIGSADFGTPDGYLQSELFSFVAPIVFLVYAI
                     GAGARAIAGEEEAGSLDVLLSTPIRRRRVLVDTFGSMTLALLGLAFLLWASTLLLGRG
                     FDMRADLAHLSAAALSTFLVALVFGSLSLAVGCLTGSRSTAIGVSSGLAVVTFLLNMF
                     GPSVPALDRVRWISPFFYYGGAEPMLHGLDPVHPVVLVGISLAA"
BASE COUNT          661 a         1670 c         1454 g          587 t
ORIGIN      
        1 agccctgcct cccttgaact cgatcaccgg tcggaggccg agcgagcgcc ctgcccaggc
       61 caccacctcc ggcacgtggc caccccggac caaccagtcc aggtcgccca ggctcgccac
      121 caaccggacc cgcgccgcca cgcgctcggc cctcgccctt acgtcatcga gcgacccgcc
      181 cagccgagcg catcccgcgg cggcgagcgc caccaacccc tgcgcccctg ccgccgtgcc
      241 cgaatccagg acgccgacgg cgtccccgaa cgagccggcg gccatgcgag cggcctgata
      301 ggagccgctc atccgggacg caacggtcac caccagggcg ccctccggcc ccaggcgagc
      361 ctcgatcgcc tccttcaggt cccccggtga gggagcggat gtggtgatgc cttcatccgc
      421 gcggcgcagc agctcgggca gggggagctc ggtgtcccgc agggtgtccg ctcctacggt
      481 gacccacatc gggaccacca ccacgcccgc ctcctcggcc acctcgtccg gaagggcggc
      541 cgcgctgtcg gtgatcacgg ccacggtcat cgatccggct cctcgagcga ccgcccaccg
      601 gggcgttcgg acccccgggg tccccgctca ccctctgctg cgggctcgtg gacccacgcc
      661 gcgctcagga acaccagcgc tccggccgac agcagcacga cgcgcgaggg ctgcagctga
      721 ccgaggaacg gccacctcac gccgttcacc aggtccacgg ccacgccggc caccagggcg
      781 gccacggcca gacccgcccg gatcgtcacg tggaacgccg tgaacgcctg gacccggtct
      841 cctccctcga gccgggtctg gagcacgttc atcccggcca cgaggctggc ggcactcgag
      901 gccccgaaga agcccgcccc gatgtaggcg atcccgatgt tgggcgacag gctcatcacc
      961 gccaccacga tcccctgcag ggccacggcc gtccgcacca tgatcagctg gtcgcgatgg
     1021 cggagcgcct gcagggtgcc cagccccgcc gcggcaccca ccccgaagag ggcgatcagg
     1081 atgccgaact gcgtgtcgct ggcctcgagc acctgccgga cgaacttcac ccccaccgag
     1141 aacagggcgc cgatgcccag ggtgatggtg gcgccggccg gcaccaccat ccggaccagc
     1201 gggatccgga aggccttgag gaacttggtc ctctcctcgg agccctgctc ctgctccggc
     1261 tgctcaccac gttcgtccag gacgtgcagc gaacggacga acgcgaagga gatcaggaag
     1321 gtgagggcat cgatcaggaa gaccagcagg taggaccggc tgccgatcgg ccccgagtgg
     1381 ccgaacagca tggcctgcag accggccacg ccgccgaagg cggcggcacc cagcgggatc
     1441 gtcccgaacg aggatcccag gatgatgccg ttcgccagcg agagctggtc ctcgtcgccc
     1501 tccacgaggt cggggatgga tgcgtcccgg gccgggagga acaccagccc tcccaactgg
     1561 aggaagaacg cccacaggta gacccaccag agggcctgca ccagcggcag caccaccgcg
     1621 accgccaccc ggaacaggtc ggcgcccatc atggtccgcc gacgccgcca gcgccccacc
     1681 acccgactgg ccacgggacc gcccaccgcc gagggcagga gctggaggac cagcacgccg
     1741 cccaccgccg tggaggagtt cgtgaggtcg aggaccagca cgatcagggc gaccgtgccc
     1801 atccagtccc cgagcgccga gaccgcctgc ccgacgaaca ggttccggaa cccccgatgc
     1861 ccgagcggga tgttcatccc gagatcacct tcggcgcggc tggtcctggt cgagcacgag
     1921 ccggttcagg tagatcctcc agcgcctcgc tccgaccgcg ggccggttcc ccagcacccg
     1981 cttcacgagg agcggaacca cgaccgagcc gccggccacc atccccgagg gcccgcgcgt
     2041 ggcccgaagc accggcacca gcgccaggta ggcgatgaag cagccgatcg atgtgcgttt
     2101 ggcaagcttc ccgcccgcca gtcccacgct cagcaccacc accccctccc agccgttcac
     2161 gaggagcgcc cccagcgccg tggacagccc ccgaccaccg gctcccctga ggaacgggct
     2221 ccagtcgtgc cccgccaccg cggctccgcc ggccagcccc gcgatcagcg gccgctcccg
     2281 gcccgcgagc agtgggccca cggccccctt ggcaacgtcc agcgtcccgg ccagggccag
     2341 ggggccgaac ccggcgatcc tgtacagccc cgaacccgag acggtccccg tccccaccga
     2401 ccgcaggtcg acgtgccgca accggtgggc caccaggttc gagaagggga tggcgccgca
     2461 caggtacgac agcgccagga cggcgggtgc cggaagcctc ctcacgccgg aaacgctaca
     2521 ccgcacggcc atctccctgg ttgggggccc gttcgcggcc cggccggtgg tggtcggcga
     2581 gcgcctcgga catggtgagc cgtccttccc tcctccagcg ggccgatcgg cccacaccgg
     2641 cgatcccgag gatcgccaga gtggatgcga ggcatccgga ggtccttacg catggacacg
     2701 gccatccgca cggaggcgct gacgaagtcc tacggccgcg ctcgtggcgt gatcgacctg
     2761 ggtttcgagg tgccccgcgg cgaggtcttc ggctacctgg gcccgaacgg agccggcaag
     2821 accaccacga tccggaccct gctcgacctc atccgaccca cctccggccg ggccgaggtc
     2881 ttgggtatgg acagccacga ggacagcctc gagatccatc ggcgcaccgg gtacctgccc
     2941 ggggagctgg ccctgtacga ccggatgacc gggtccgact acctccagca cctggcggcg
     3001 ctccggggcg gcgtggagtg ggcgtacgtt cgggacctgg ccggccggct ggactgcgat
     3061 ctgggcgcgc ggatccgctc cctctcgcac ggcaaccggc ggaaggtggc gctgatccag
     3121 gcgttcatgc accggccgga cctcctcgtc ctcgacgagc ccacccaggg cctggacccg
     3181 ttggtccagc gcgagttcca tcatctggtg acccaggtgc gtgaccgggg ctgcaccgtg
     3241 ttcctgtcct cgcacgtcat gcccgaggtc gagcgtctgt gcgaccgggt ggggatcatc
     3301 cgggaaggcc ggctgatcgc ggtggaggac atcggggacc tcaaggcgcg ctcgatccgg
     3361 accctggacg tgcacttcgc gcaccccgtg gaccccctgc acttcgcggg gctccccggc
     3421 gtgaaggagg ccacgcagca cggtgacgcg gtccggatca ccgtggccgg tccgctggat
     3481 gccgtggtga aggccgtcgc gacgttcccc gtcgtggaac tcaccagcca cgagccctcc
     3541 ctggaggagg tcttcctcac cttctacggc aagcgggagg acgacgatgg cgcgtgacct
     3601 cgccctgcgt ccccacgggg tgtggctcgg aacggtcttc gcgaggaccc tccgggagca
     3661 gcggcgagcg ctgctgtggt ggacgctcgg cgtggtgggg accgtgctga tgtatgcggc
     3721 gatctggccg acgatccggc agaacgcggt ggatctgaac cgctacctgg actcgttccc
     3781 cgacgcgatg cgcaacctga tcggctcggc cgacttcggc acgcccgatg ggtacctgca
     3841 gtcggagctg ttcagcttcg tcgcgccgat cgtcttcctg gtctacgcga tcggcgcggg
     3901 cgcccgggcc atcgccggcg aggaggaggc gggctccctc gacgtcctgc tgtcgacgcc
     3961 catccgccgc cgccgggtcc tggtggacac gttcggctcg atgaccctgg ccctgctcgg
     4021 gctcgcgttc ctgctgtggg cctccacgct gctgctcggc aggggcttcg acatgcgggc
     4081 ggacctcgcc cacctctcgg ccgccgccct gtccaccttc ctcgtggccc tcgtcttcgg
     4141 gagcctgtcg ctcgccgtcg gatgcctcac ggggagccgg agcacggcga tcggggtctc
     4201 gagcgggctc gccgtggtga cgttccttct caacatgttc gggccgtcgg tgccggcctt
     4261 ggaccgggtc cggtggatct ctcccttctt ctactacgga ggggccgagc ccatgctcca
     4321 cggcctggat ccggtgcacc cggtggtctt ggtcggcatc tccctggccg cg
//
LOCUS       DASQQQ010000035         3398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_251104, whole genome shotgun sequence.
ACCESSION   DASQQQ010000035 DASQQQ010000000
VERSION     DASQQQ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251104"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(366..1421)
                     /locus_tag="VE646_01830"
     CDS             complement(366..1421)
                     /locus_tag="VE646_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HZD78765.1"
                     /translation="MIRASHRDIIVIGASAGGVTALKGVIRELPPDLPASLFVVLHTV
                     PRGVSQLAPILDAAGPLRASFAEDGEGIRYGHVYVAPPDRHLLLRDSHVHLTRAPKEN
                     RTRPAVNPLFRSAAAAYGRRVVGVVLTGTLDNGTAGLLAVEAGGGTTVVQDPEDAEFP
                     GMPLSALDYLDPDHVVTAAELPGLLVELVGEEMAESGPERLDGAVVNNPGPDDSIGTH
                     QRGPGPDLTELGSREVRPPNTASGFTCPECSGALWEVEERGLTEYVCRIGHLFGPETL
                     LEEHAERRDDLLETALRSIEEESALTARLLERGRERGLSEHRLRRHVQRIRTLERTAA
                     SLEHLLREESPPAEAAE"
     gene            complement(1434..2066)
                     /locus_tag="VE646_01835"
     CDS             complement(1434..2066)
                     /locus_tag="VE646_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HZD78766.1"
                     /translation="MAPSAGKAPRYAMVVIGGSAGALGPMRMVLSGLPPGFGAALAIV
                     LHRVPEGPSLLREILGRKTALEVVDATEGTRMAPGRVLIAPADRNLVLEPESAVGFRV
                     GETHHLRSAIDPVLESAARVYGAGAIALILSGGGHDGAEGCRAVHDAGGTVVVQDPAT
                     AVAGDMPRSVIATGVADVVCDPAEMAGILMGLVSGGSPADVVKPRGGTAP"
     gene            complement(2167..2532)
                     /locus_tag="VE646_01840"
     CDS             complement(2167..2532)
                     /locus_tag="VE646_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78767.1"
                     /translation="MADRDTAERLDALERGLAELKRRMDAELARRDGAGSGEPQHEEI
                     RAEAREQAARARERAHHVHARARELTEQAETLHGTTARAHEAQQQNEDGLRRTAQRER
                     DGAESGAGPSVGPAGNRRR"
     gene            2789..>3398
                     /locus_tag="VE646_01845"
     CDS             2789..>3398
                     /locus_tag="VE646_01845"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HZD78768.1"
                     /translation="MGGSEEQRTALLDYIREQRGVDFASYKRTTLKRRLEKRLQAIGA
                     SDYGEYLDVLQASPEEFRGLLDTLFINITQFFRDPKAWNVLRRQLEGRLDPNAERPVR
                     VWCAGVASGEEAYSVAMLLSEILGTDGFLGRAKIYATDVDDDALIQARQGAYSRKQVA
                     DIPEELRARYMEEQAGVYTFRKDLRRALIFGHHDLLEDAPISK"
BASE COUNT          478 a         1303 c         1130 g          487 t
ORIGIN      
        1 cagcgcccgc gagccttcgg ccgcccggcg gtaatgggag atctcgcaga acgtcgcggg
       61 cggccgttgg cgtgcaggaa cgagcaccag gccagcgcga acgtgcggcg tcaatcggat
      121 cgcagcccca cccgccagag gacggatggg cggcctcgtt ccccctcacc acccggcacg
      181 cgaccctttc accggccatg cggaggagac acagggccac tgccgtggaa accgccgggg
      241 gcgtgggaac cccgggacgg gcgcaccgca acggcggaat cgtactccgg gtccttacgg
      301 tagggaacgc ccgggtgccc ggtccggccg gccatccgcc gctggccggc cggctgtcgg
      361 gaccctcatt ccgcggcctc cgcaggcgga ctctcctccc gcagcaggtg ctcgagggat
      421 gcggcggtgc gctccagcgt ccggatccgt tggacgtgcc gccgcaggcg gtgctcgctc
      481 agaccccgct cccgcccccg ctccagcaga cgggcggtga gggcggactc ctcctcgatc
      541 gagcggaggg cggtttccag caggtcgtcc cgccgctccg cgtgctcttc gagcaaggtc
      601 tccggaccga agaggtggcc gatgcggcag acgtactccg tcagcccccg ctcctccacc
      661 tcccacaggg cgccgctgca ttccggacag gtgaaccccg aagcggtatt cgggggccgg
      721 acctcccggc tgccgagctc cgtcagatcc ggtccggggc ccctctgatg cgttccgatc
      781 gaatcatccg ggccggggtt gttcaccacc gcaccatcca gccgttccgg tcccgattcg
      841 gccatctctt cgcctaccag ctccaccagc aggcccggaa gctccgccgc ggtcaccacg
      901 tggtcggggt ccaggtagtc cagcgccgag agcggcatcc ccgggaactc ggcgtcctcc
      961 gggtcctgga ccaccgtggt gcctccgccc gcctccaccg cgaggaggcc ggccgtgccg
     1021 ttgtccaacg tcccggtcag taccacgccc accacccgtc gaccgtaggc cgcggccgcc
     1081 gaccggaaca ggggattgac cgccggccgc gtgcggttct ccttgggtgc gcgggtcagg
     1141 tgtacgtggc tgtcccgcag cagcaggtgg cggtccggcg gtgccacgta gacgtggccg
     1201 tagcggatgc cctccccatc ctccgcgaac gaggcccgga gtggacccgc ggcgtccagg
     1261 atcggggcca gctgcgacac gcctctcggg accgtatgca gcacgacgaa cagcgaggcc
     1321 ggcagatccg gcgggagctc ccgaatcacg cccttcagcg cggtgacccc cccggccgag
     1381 gccccgatga cgatgatgtc ccgatggctt gctcggatca ttccgcgcct cccctacggg
     1441 gccgtgccac cccgaggctt caccacgtcc gccgggctcc caccgctcac cagccccatc
     1501 aggatacccg ccatctcggc cggatcgcat accacgtcgg cgacgcccgt ggcgatcacg
     1561 gatcgtggca tgtcgcccgc caccgccgtg gccggatcct gcaccaccac cgtgccgccc
     1621 gcgtcgtgca ccgcgcgaca gccctccgcg ccgtcgtggc cgccgccgct caggatcagc
     1681 gcgatcgccc ccgccccgta gacccgggcc gcggattcca gcaccgggtc gatcgccgac
     1741 ctcaggtggt gggtttcccc cacccggaac cccacggcgc tttcaggctc gagcaccagg
     1801 ttgcggtccg ccggggcgat cagcacccgc cccggggcca tccgggtgcc ctccgtcgcg
     1861 tccaccacct ccagggccgt cttgcgaccc aggatctccc gaagcaggct cggcccctcc
     1921 ggcacccggt gaagcacgat ggcgagcgcc gcgccgaatc ccggtggaag cccggacagg
     1981 accatccgca tcggacccag agcgccggcc gatccgccga tcaccaccat cgcgtagcgg
     2041 ggagccttcc ccgctgaggg agccattgag ggcctcgtgc ccgggacggg ggcgagccgc
     2101 cccgattgga cgacccgcgt tcctgcaacg tgataccccg cagcgccgag cgccagtcga
     2161 tcgggctcac cggcggcgat ttccggccgg gccgaccgag ggcccggccc cgctttccgc
     2221 cccatccctc tcccgctggg ccgttcgccg tagcccgtcc tcgttctgct gctgtgcctc
     2281 gtgcgcccgg gccgtcgtcc catggagcgt ctcggcctgc tcggtcagct cacgggcgcg
     2341 agcatgcaca tgatgcgcgc gctcgcgcgc tcgcgcggcc tgctcccggg cctccgcccg
     2401 gatctcttcg tgctgcggct ctcccgaccc ggccccatcc cgccgcgcca gctccgcgtc
     2461 catccggcgc ttcagctcgg caagcccccg ctccagcgcg tccagacgct cggccgtatc
     2521 ccggtctgcc atacccgccg cctttcctct cggagtcgtt gggcgagcgg atgcacaccg
     2581 cgcaccagcg cgcggcgagg cacgacccgg ccaccggccg cgagaggtac tgaaaatggg
     2641 ggagtccggg ccgcgacgaa gattgacact tttccggcgt atccacacct tgtccctctg
     2701 gcgccgtccc gcgcggcgtc actcgctctc gtgcttccgc cgatgccggc gccgcggggg
     2761 cgttccacga atctctcccc ggaggcccat gggcggctcc gaagagcagc ggacggcgct
     2821 gctcgactac atccgcgagc aacgcggcgt cgatttcgcc tcctacaagc gcacgacgct
     2881 gaaacgccga ttggagaagc ggctccaggc gatcggggcc tcggactacg gtgagtacct
     2941 ggatgtgctc caggcctcgc ccgaggagtt tcgggggctg ctggatacgc tcttcatcaa
     3001 catcacgcag ttcttccgcg atccgaaagc ctggaatgtg ctgcgccggc agctggaggg
     3061 gcggctggac ccgaacgccg agcgtccggt gcgggtgtgg tgcgcggggg tggcgagcgg
     3121 cgaggaggcc tacagcgtgg ccatgctgct ttcggagatc ctgggcacgg acggattcct
     3181 gggccgggcc aagatctacg cgacggatgt cgacgatgac gcgctgatcc aggcacggca
     3241 gggcgcgtac agcaggaagc aggtggcgga catccccgag gagctccggg ctcgctacat
     3301 ggaggagcag gccggggtgt acacgttccg caaggacctg cgccgcgcgc tcatcttcgg
     3361 gcaccacgac ctgctcgagg acgcgccgat ctcgaagc
//
LOCUS       DASQQQ010000036         4100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_85297, whole genome shotgun sequence.
ACCESSION   DASQQQ010000036 DASQQQ010000000
VERSION     DASQQQ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85297"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2092
                     /locus_tag="VE646_01850"
     CDS             <1..2092
                     /locus_tag="VE646_01850"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HZD78769.1"
                     /translation="VGEPGPADAAGERLQELVQAAPGEPARHADRGGHQTHATHDKHA
                     GHSVAMFRDRFWLSLLLTIPVLFWSGMLQDWFGYTAPTFFLSDRIPAIVGTVLFLYGG
                     LPFLRGGVREIGDRLPGMMLLISLAILVAFGASIASEFDLIDLRFWWELALLVDVMLL
                     GHWQEMNAIGQASGALEALAALLPDEAEVVHGSQVHTVAAAELRPGDLVLVRPGARVP
                     ADGTIVEGGAEFDESMITGESRPISRGEGERVVAGTVATDSSVRVKVDAVGEQTALAG
                     IRRLVEAAQASRSRSQALADRAAAVLFYVAVAAGVVTFVAWALLGNASDAVERSVTVL
                     VISCPHALGLAIPLVVAISTAISARGGVLVKDRLALERMRTVDTVLFDKTGTLTEGAP
                     AVSEAAGVGGAEDEVLLLAAAAEASSEHPLGRAIVAAAGERGRIPEGTAFRSSTGRGV
                     SATVDGARIEVGGPALLRERGLDEPSPIAARVSSWRSRGATVLFVIREGAVIGALAAE
                     DRVRPESVEAVRALHADGLRVVMITGDAEEVARAVAKHIGIDEVMAEVLPEDKAAAVA
                     ELSSRGLSVAMVGDGVNDAPALAGADVGIAIGAGTDVAIESAGVVLAGDDPRAVVSVR
                     RLSKASYRKMIQNLGWAVGYNVIAIPLAAGVLAWAGVVLAPAVGALAMSLSTVIVALN
                     ALLLRRLDLRPGPA"
     gene            2164..2553
                     /locus_tag="VE646_01855"
     CDS             2164..2553
                     /locus_tag="VE646_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78770.1"
                     /translation="MPYPIVVVDRSEVREGRLEDLKSAAKGLAEFVIANEPRPYSYTF
                     YFDEDATHMTVLQVHPDSASMEEHMSVAADQFPPFAELLEMRSMEVSGDPSEALLERL
                     RRKADMLGTVSLTVHRPHAGFARFAER"
     gene            2795..>4100
                     /locus_tag="VE646_01860"
     CDS             2795..>4100
                     /locus_tag="VE646_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HZD78771.1"
                     /translation="MRVDGYPLIGDYGVVGDGRTAALISTDGAVEWLCLPDLDSPSVF
                     GAMLDAARGGRFELAPEPPFETDRRYLPGTNVLETTHRTSTGVVRVIEAFTLEAGIPP
                     PWRELVRRVEGLEGTVELRWSVEPGFDYGARAATFTEMAEGFVADGPACRIGVLAWDA
                     GVPEVRGDGAVLAGRFSTGPGSRSTLALVGDGRRPLRLPSRGSVERRLEGTIRDWRDW
                     ISRSTYRGQWAGAVERSLLALKLLQNPETGAIAAAATASLPERIGGDRNLDYRFGWIR
                     DIAFTLEALMRLGFEDDAHRSLLWLLDAVAGSHPRPAPLYALGGGPAPSSRELPLPGY
                     RGSRPVMIGNQAVSQLQLSGWGDLLQAVWMHVERGANVLDARTGERLADLADVLAATW
                     PHPDAGLWELREPAQFANSKMGCWVAFDRILNLVDWGQVPPRH"
BASE COUNT          504 a         1402 c         1582 g          612 t
ORIGIN      
        1 ggtcggcgag cccggacccg ccgatgccgc cggggagcgg ctccaggagc tcgtccaggc
       61 ggctcccggc gagcccgccc ggcacgccga tcgcgggggt caccagaccc acgcgaccca
      121 cgacaagcac gcgggccaca gcgtcgcgat gttccgcgac cggttctggc tgagcctgct
      181 cctcacgatc ccggtgctgt tctggtccgg gatgctgcag gactggttcg gctacaccgc
      241 ccccacgttc ttcctctcgg accggatccc ggccatcgtg gggaccgtcc tcttcctcta
      301 tggggggctg ccgttcctcc ggggcggcgt ccgcgagatc ggggaccgcc tgcccggcat
      361 gatgctgctg atctcgctgg ccatcctggt ggcgttcggc gccagcatcg cctcagagtt
      421 cgacctgatc gaccttcggt tctggtggga gctggccctg ttggtcgatg tcatgctcct
      481 cggccactgg caggagatga acgcgatcgg gcaggcctcc ggcgccctcg aggccctggc
      541 agcgctcctg cccgacgagg cggaggtggt ccacggttcg caggtccaca cggtcgccgc
      601 ggccgagctc cggcccgggg atctggtcct ggtgcgaccc ggagcccggg tgcccgcgga
      661 cgggaccatc gtcgaaggcg gggcagagtt cgacgagtcg atgatcacgg gggagtcacg
      721 tccgatctcc cgaggagagg gggagcgggt ggtcgccgga accgtggcca ccgacagctc
      781 ggtccgggtg aaggtggacg cggtgggcga gcagaccgcg ctggccggta tccgccggct
      841 ggtggaggcg gcgcaggcct cccggtcccg gtcgcaggcc ctggccgacc gggcggcggc
      901 cgtcctcttc tacgtggcgg tggcggccgg cgtggtcacc ttcgtggcct gggccctgct
      961 cgggaacgcc tccgatgccg tggagcggag cgtcaccgtc ctggtcatct cgtgtcccca
     1021 cgcgctcggc ctggcgatcc ccctggtcgt cgccatctcc accgcgatct ccgcccgggg
     1081 tggcgtgctg gtgaaggacc ggctggccct cgagcggatg cgcacggtgg acaccgttct
     1141 cttcgacaag accgggaccc tcaccgaagg tgcccccgcg gtcagcgagg cggctggcgt
     1201 gggcggggcg gaagatgagg tcctccttct cgccgctgcc gccgaggcca gcagcgagca
     1261 ccccctgggc cgggccatcg tcgccgcggc cggggaacgg gggaggatcc cggaggggac
     1321 ggccttccgc tcctccacgg gacgaggggt gtcggccacc gtggacggcg cgcggatcga
     1381 ggtgggcgga ccggcgctgc tccgggagcg gggcttggac gagccgtccc ccatcgctgc
     1441 ccgggtgtcg agctggcgct cccggggggc gacggtcctg ttcgtgatcc gcgagggcgc
     1501 ggtgatcggc gcgctggcgg ccgaggatcg ggtgcgcccg gagtcggtgg aggccgtccg
     1561 cgccctccac gccgacgggc tgcgcgtggt catgatcacc ggcgatgccg aggaggtcgc
     1621 gcgcgccgtg gcgaagcaca tcggcatcga cgaggtgatg gccgaggtcc tgcccgagga
     1681 caaggccgcg gcggtggccg agctgtcgtc ccgaggcctg agcgtggcga tggtcggcga
     1741 cggcgtgaac gatgccccgg cgctggccgg cgccgacgtc gggatcgcca tcggtgccgg
     1801 cacggatgtc gcgatcgagt ccgcgggggt ggtgctggcc ggcgacgacc ctcgcgccgt
     1861 ggtctcggtc cggcgcctgt cgaaggcgag ctaccggaag atgatccaga acctgggctg
     1921 ggccgtcggg tacaacgtga tcgccatccc cctcgccgcc ggggtgctgg cgtgggcggg
     1981 cgtggtcctg gcgccggccg tcggggcgct ggccatgagc ctgtccacgg tgatcgtcgc
     2041 cctcaacgcc ctgctcctcc gacggctgga cctccgcccg gggccggcct gagcttgcga
     2101 tacgcgagcc ggtctccgta ggttggagcg gcagaggggc cgggacacag ggaggtgggg
     2161 cccatgccct atccgatcgt cgtggtggac cgttccgagg tccgcgaggg caggctggag
     2221 gacctgaaga gcgctgcgaa ggggttggcc gagttcgtga tcgcgaacga acctcggccg
     2281 tactcgtaca ccttctactt cgacgaggac gccacgcaca tgacggtcct ccaggtgcat
     2341 ccggactccg cctcgatgga ggagcacatg tctgtggcgg ccgaccagtt ccccccgttc
     2401 gccgagctcc tggagatgcg atcgatggag gtctccggcg atccgagcga agccctgctg
     2461 gagcggctgc ggcgcaaggc cgacatgctc ggcaccgtga gcctgaccgt gcaccgtccg
     2521 cacgccgggt tcgcccggtt cgcggagcga taggggatat cagccggcga ccggttcctc
     2581 cctcaaccgc tccgcggtgg cgcgggcgag gtcctcgagc cgcggtctct cgtcctgggt
     2641 ccaggacctg ggtcgtcggt ggtgcgcgga taccatcgcc atgaccccgc cggtcctgga
     2701 caggaccggg atggatgcca cggcacgcga gctcggtcgg ggtgtcgccg ggccggcctc
     2761 gtttgccgag ggaagggctg gatagaaaag ccacgtgcgc gtcgacggct atcccttgat
     2821 cggcgactac ggcgtggtcg gcgatgggag gacggccgcg ctgatctcca cggacggggc
     2881 ggtggagtgg ctctgcctgc ccgacctgga ctcacccagc gtgttcgggg cgatgctcga
     2941 tgctgctcgg ggcggtcgct tcgagctcgc gcccgaaccg cccttcgaga ccgaccgccg
     3001 gtacctgccg gggacgaacg tgctcgagac gacccaccgg acctcgaccg gggtggtccg
     3061 ggtcatcgag gccttcacgc tcgaggcggg catcccgccg ccctggcggg agctggtgcg
     3121 acgggtggag ggcctggagg ggacggtgga gctccggtgg tcggtcgagc ccggcttcga
     3181 ctacggggcc cgggccgcca ccttcaccga gatggccgag ggcttcgtgg ccgacggccc
     3241 cgcgtgccgg atcggcgtgc tggcgtggga cgcgggggtg cccgaggtcc gcggcgacgg
     3301 ggccgtgctg gccggccgct tctccaccgg tccgggcagc cggagcacgc tcgcgctggt
     3361 gggcgacggg cgcaggccgc tgcggcttcc ctcccgcggc tcggtggagc ggcggctgga
     3421 gggcaccatc cgggactggc gggactggat ctcccgcagc acgtaccggg gtcagtgggc
     3481 gggagccgtg gagcggagcc tgctcgcgct gaagctgctc cagaaccccg agaccggcgc
     3541 gatcgccgcc gccgccaccg cctcgctgcc cgagcggatc ggcggcgacc ggaacctgga
     3601 ctaccggttc ggctggatcc gcgacatcgc cttcaccctc gaggcgctga tgcgcctggg
     3661 gttcgaggac gatgcgcacc gctcgttgct ctggctgctc gacgcggtgg ccggctcgca
     3721 cccgaggccg gcgcccctgt acgcgctcgg tggtggtccg gcgcccagct ccagggagct
     3781 cccgctgccg ggctaccggg gctcgagacc ggtcatgatc ggcaaccagg cggtgtccca
     3841 gctccagctc tcgggctggg gagacctgct ccaggccgtg tggatgcacg tggagcgcgg
     3901 ggcgaacgtg ctcgacgcgc ggacggggga gcgcctggcg gacctggccg acgtgctggc
     3961 cgcgacctgg cctcacccgg acgccgggct gtgggagctg cgcgagccgg cccagttcgc
     4021 gaactcgaag atgggctgct gggtggcgtt cgaccggatc ctgaacctgg tggactgggg
     4081 ccaggtccca cctcggcacg
//
LOCUS       DASQQQ010000037         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_140815, whole genome shotgun sequence.
ACCESSION   DASQQQ010000037 DASQQQ010000000
VERSION     DASQQQ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140815"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(429..1559)
                     /locus_tag="VE646_01865"
     CDS             complement(429..1559)
                     /locus_tag="VE646_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZD78772.1"
                     /translation="MATDRFRREFELEGGSVWLNTAHQGRLPRRAALALAEAVRWKLH
                     PEMLATPERFSEVPSRLRQALGRVLGAREDEIVLANSASYGLQLIANGLDLGPGDEVV
                     VAANDFPSDILPWLLLRDRGVTIRMIEPQDEVLTPDEVQAALTHRTRVVCLTWVHSFS
                     GQVADLQGVGRACRSRGAWMVVNGSQAVGAMPIDVQALPVDALVSAGFKWLCGPYGTG
                     VCWLRPELADALRPTKLYWLSALTADDLAAPSLDLESITPRRTGRLDVFGTANFFNYV
                     PFTAALELLLELGIEEVAAYVDGLVTGLLAGIDRARFRLVSREDVRSPLVVVEPLQEA
                     SGEVFARLATAGVHVAHRRGRIRISPHLYNTPDEINRALELL"
BASE COUNT          286 a          751 c          683 g          302 t
ORIGIN      
        1 aggtcggacg cggctcggtg gtgaccctgg accttccggc cacggtccgc caaggggtgg
       61 ctcgccgcct ccagcagcag gtcgagcaga tcggcaggct cattcccgtc agtccttcct
      121 gagcccgccg tcccgggcgg cgtccctggc cgagcacgcc ccggacgccg gccccgccga
      181 gccgagctga accgcaacca cgtgcgcggc tggaccgccg cgatgccgcg gtcctctggc
      241 tgctggcgga tgcgggcctg cggtcgaccg ggctgaccga cctcaaggtg ccggacgtcg
      301 atcttcgcgg ccggaagggt ctccgtacgc cccgctccgg cgggccgctc gctttgctgg
      361 gagcaagctg cgccagagtc tttggctccg ggccagcgtg cccaacctct cgccggagat
      421 gaccccgctc acaacagctc gagcgcgcgg ttgatctcgt ccggggtgtt gtacaggtgc
      481 ggggagatac ggatgcgacc gcggcggtgc gccacgtgca cccccgccgt ggcgagccgc
      541 gcgaacacct ccccggacgc ttcttggagc ggctccacca cgaccagcgg cgagcggacg
      601 tcttcccggg acacgagccg gaaccgcgcc cggtcgatcc cagcgagcag gccggtcacc
      661 aggccgtcga cgtaggccgc cacctcctcg atccccagct ccagcagcag ctccagcgcg
      721 gcggtgaacg ggacgtagtt gaagaagttc gccgtgccga acacgtccag ccggcccgtc
      781 cgcctgggcg tgatcgactc caggtccagc gagggcgccg ccaggtcgtc ggcggtgagc
      841 gcgctcaacc agtacagctt ggtgggccgc agagcgtcgg ccagttcggg ccgcagccag
      901 cacaccccgg tcccatacgg gccgcacagc cacttgaagc cggcgctcac cagcgcgtcg
      961 accggcagtg cctgcacgtc gatgggcatg gcgcccaccg cctgggaacc gttgaccacc
     1021 atccaggcgc cgcggctgcg gcaggcccgc cccacgccct gcaggtcggc cacctgccct
     1081 gagaacgagt gcacccaagt caggcagacc acccgggtgc ggtgcgtgag cgcggcctgc
     1141 acctcgtctg gggtgagcac ctcgtcctgc ggctcgatca tgcggatcgt gaccccccga
     1201 tcccgcagca gcagccacgg caggatgtcg gatgggaagt cgttggcggc caccaccacc
     1261 tcgtctcctg gccccagatc cagcccattg gcgatcagct gcagcccgta ggaggcgctg
     1321 ttggccagga cgatctcgtc ttcgcgggct cccaggaccc gccccagcgc ctggcgtagc
     1381 cgggagggga cctccgagaa ccgttccggg gtggccagca tctccgggtg gagcttccac
     1441 cgcaccgcct cggccaaggc cagggcggcc ctgcggggca gccgcccctg gtgggcggtg
     1501 ttgagccaca ccgaaccgcc ctcgagctcg aactccctcc ggaacctatc ggtcgccatg
     1561 gccggtagtc tgccatccga tcggcggcgg tcgctcagcc tgcgctcccc gctggcccct
     1621 ggcgagccaa acgcccaggt cgcgtagcgc tccctgattc ttaggactcc cctatgtcat
     1681 tctcagcgag ttctcagggt tcccgcgtag aaccacctca acgcggcgga tgcgcccacg
     1741 gctggatggt cccggtgccc ccggttccga ggcgaggctc agcccctcgg atgaccgggt
     1801 tcacggtggg ctccgccgcc ggcgacacgc cggaggccca tatggtggga caaggccggg
     1861 acaaccgttc cggccttggg gcctccgggg aagtcgtggg gttccgggtg gtgctggatc
     1921 gcgtggctgg cgctatggtg tgactggtct acgtcgtcgg tccctcgatt cctttacgtc
     1981 cccgtcttcg tccgcagcca acgacccgtc cgggttccag ga
//
LOCUS       DASQQQ010000038         3169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_113287, whole genome shotgun sequence.
ACCESSION   DASQQQ010000038 DASQQQ010000000
VERSION     DASQQQ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113287"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /gene="hutU"
                     /locus_tag="VE646_01870"
     CDS             <1..1437
                     /gene="hutU"
                     /locus_tag="VE646_01870"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HZD78773.1"
                     /translation="ETLLVQSGKPVGVFRTHEMAPRVLISNAMLVPKWADWDTFRDLE
                     HRGLTMYGQMTAGSWIYIGTQGILQGTYETLAEAATQRFQGSLAGTITLTGGLGGMGG
                     AQPLAVTMNGGVALCVEVDPARIERRIKTRYLDRETDDLDQAVAWVEQARTEREPLSV
                     GLLGNAAELLPALLKGGFSPDLVTDQTSAHDPVGGYIPAGLTLEAAAELRGGDPEEYQ
                     RRAFASMARHCEAMVGFLDAGAVVFDYGNNLRAGAERGGFERAFDYPGFVPAFVRPMF
                     CEGKGPFRWAALSGDPEDIATTDRAVMELFPNDERLHRWLHMAQDRVAYQGLPARICW
                     LGYGERAAAGLRFNELVASGELSAPIVIGRDHLDSGSVASPYRETEGMRDGSDAIADW
                     PILNALVNTAAGAHWVSVHHGGGVGIGYSIHAGMVVVADGSSNAAERLERVLTTDPGT
                     GVVRHADAGYEQALAVAKERGVRLPWLE"
     gene            complement(1460..1957)
                     /locus_tag="VE646_01875"
     CDS             complement(1460..1957)
                     /locus_tag="VE646_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZD78774.1"
                     /translation="MADTEQTVEVRPIREEDRDWVRRLMRDRWKSERMVAHGQVFEPA
                     AMDGFVAEIAGEAVGAITFRIQDKGCEVVSLDALREGLGIGTELIRQVTAAAREAGCR
                     RVWAMTTNDNADAIGFFGRRGFEITAVHEGAVDEARKLKPEIPLVGHDGVEIHDEVEL
                     ELKLR"
     gene            complement(2005..>3169)
                     /locus_tag="VE646_01880"
     CDS             complement(2005..>3169)
                     /locus_tag="VE646_01880"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HZD78775.1"
                     /translation="APTAALRAAAVALPFVAIAQVILGGSRGLKIMRDTLVAYWVGQP
                     ISWIVFSLIAWTFSKTVTATVATYGLSWLFATAVAWVMWHRDTGGLDHQPAEPGEVNA
                     LMRYGAPRAPAALLSQALFYTDFFVLSANTAGPSLGVYAASVRVAQALVLFLTAVSYM
                     FSPFVADLHERGERDRLNVLFKAITRWTLAGTIPLLLLFMVAPGPILQVFGAKFQTGT
                     SWLRILLVGQILNVSVGAAGFVLIMAGRTGWDLIVYASSFLLDLGMSLVLVPRFGPTG
                     AAVAQASTLAASNAFRLFLVWRFVHIQPYDRHYARLAIPAAAATAVMLGVHGVLASKA
                     WPVDLFGTGLVGGVAYLVVLVAAGLTPVEKRTILRVARRRPVAPSDEGSPLNG"
BASE COUNT          489 a         1140 c         1118 g          422 t
ORIGIN      
        1 gagacgttgc tggtgcagtc tggcaagccc gtgggcgtct tccggaccca cgagatggcc
       61 cctcgggtcc tgatctccaa cgcgatgctc gtgcccaagt gggccgactg ggacaccttc
      121 cgcgacctcg agcaccgggg cctcacgatg tacggtcaga tgaccgcggg gtcctggatc
      181 tacatcggca cccaggggat cctgcagggc acctacgaga cgctggccga ggccgccacc
      241 cagcgcttcc agggctcgct cgccgggacc atcaccctga ccgggggcct gggtggcatg
      301 ggcggcgccc agccgctcgc ggtgacgatg aacggcggcg tggccttgtg cgtagaggtc
      361 gacccggccc gcatcgagcg ccgcatcaag acccggtacc tggaccgcga gaccgacgac
      421 ctggatcagg cggtggcctg ggtggagcag gccaggaccg agcgggaacc gctctcggtg
      481 ggcctgctcg ggaacgccgc cgagctgctg ccggcgctgc tgaagggggg cttctccccc
      541 gacctggtca cggatcagac ctcggcgcac gacccggtcg gcggctacat cccggccggc
      601 ctcaccctcg aggcggcggc cgagctcagg gggggcgacc ccgaggagta ccagcggcgc
      661 gccttcgcct cgatggcccg gcactgcgaa gccatggtgg ggttcctcga tgccggcgcc
      721 gtggtcttcg actacgggaa caacctccgg gcgggagccg agcgcggcgg gttcgagcgc
      781 gccttcgact acccgggttt cgttcccgcc ttcgtgcggc cgatgttctg cgagggcaag
      841 ggccccttcc ggtgggccgc cctgtccggc gatcccgagg acatcgccac caccgaccgt
      901 gcggtgatgg agctgttccc gaacgacgag cggctccacc ggtggctgca catggcgcag
      961 gatcgggtgg cctaccaggg actgccggcc cggatctgct ggctgggata cggggagcgt
     1021 gccgcggccg ggctccgctt caacgagctg gtggcctcgg gcgagctgtc cgcgccgatc
     1081 gtgatcggcc gggaccacct ggacagcggc tcggtggcga gcccctaccg cgagacggag
     1141 gggatgaggg acgggtcgga tgcgatcgcc gactggccga tcctgaacgc gctcgtgaac
     1201 acggccgccg gggcgcactg ggtgagcgtc caccacggcg gtggtgtggg gatcgggtac
     1261 tcgatccacg cggggatggt ggtggtggcc gacggctcgt ccaacgccgc ggaacggttg
     1321 gagcgcgtgc tcaccacgga ccccgggacc ggcgtggtgc gccacgccga cgccggctac
     1381 gagcaggcgc tggcggtggc gaaggagcgc ggcgtccggc tgccctggct cgagtgaggg
     1441 cccccggtga gggtcccggt cagcgcagct tgagctcgag ctccacctca tcgtggatct
     1501 cgaccccgtc gtgccccacg agggggatct cgggcttgag cttgcgggcc tcgtccacgg
     1561 ctccctcgtg gaccgccgtg atctcgaacc ctcgccgccc gaagaacccg atcgcgtcgg
     1621 cgttgtcgtt ggtggtcatg gcccacaccc gccgacagcc ggcctcccgg gccgccgcgg
     1681 tcacctggcg gatcagctcg gtgccgatcc ccagcccctc gcgcagcgcg tccagcgaga
     1741 ccacctcgca ccccttgtcc tggatccgga aggtgatggc gccgaccgcc tcgcccgcga
     1801 tctcggccac gaatccgtcc atggccgcgg gctcgaagac ctgcccgtgg gccaccatcc
     1861 gctcgctctt ccagcgatcg cgcatcaggc gccggaccca gtcccggtcc tcctcgcgga
     1921 tggggcggac ctccaccgtc tgttccgtgt cagccatccc tctcccccac atcaagcgga
     1981 ccagctcgac accgacgcac caccctaccc gttgagcggt gacccttcat ccgagggcgc
     2041 caccggccgg cggcgcgcca cacgcaggat cgtgcgcttc tccaccgggg tcagccccgc
     2101 ggccaccagc accaccaggt aggccacccc gcccaccagg ccggtgccga acaggtccac
     2161 aggccaggcc ttggaggcca gcacgccgtg gaccccgagc atgaccgccg tggcggccgc
     2221 cgcggggatg gccagccgag cgtagtgacg gtcgtacggc tggatgtgca cgaaccgcca
     2281 caccaggaac aggcggaacg cgttcgaggc ggccagggtg ctggcctgcg ccacggcggc
     2341 gccggtcggg ccgaaccggg ggaccaacac cagcgacatc ccgaggtcga ggaggaagga
     2401 ggatgcgtac acgatcaggt cccagcccgt gcgaccggcc atgatcaaga cgaaccctgc
     2461 cgcacccacg gacacgttca ggatctggcc caccagcagg atgcgcagcc acgacgtccc
     2521 ggtctggaac ttcgccccga acacctgcag gatcggcccc ggcgccacca tgaacagcag
     2581 gagcaggggg atcgtgccgg ccagcgtcca ccgggtgatg gccttgaaca gcacgttcag
     2641 ccggtcccgc tccccgcgct cgtgcaggtc ggccacgaac gggctgaaca tgtagctcac
     2701 ggcggtcagg aacaggacca gggcctgcgc cacccgcacg gaggccgcgt acacgcccag
     2761 cgagggcccg gcggtgttcg ccgacaggac gaagaagtcg gtgtagaaga gggcttggga
     2821 cagcagtgcc gcgggcgccc tcggcgcccc gtagcgcatg agggcgttca cctcgcccgg
     2881 ctcggcgggc tggtggtcga ggcctccggt atcccgatgc cacatgaccc acgccaccgc
     2941 ggtggcgaac agccaggaca ggccgtacgt ggccacggtc gcggtcaccg tcttgctgaa
     3001 ggtccaggcg atcagcgaga agacgatcca cgagatcggt tggcccaccc agtaggccac
     3061 caacgtgtcg cgcatgatct tcaagccccg gctgccgccc aggatcacct gcgcgatggc
     3121 cacgaagggc agggccacgg cggccgcccg caacgcggcg gtgggggcg
//
LOCUS       DASQQQ010000039        17493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_30539, whole genome shotgun sequence.
ACCESSION   DASQQQ010000039 DASQQQ010000000
VERSION     DASQQQ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 17493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17493
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30539"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VE646_01885"
     CDS             <1..507
                     /locus_tag="VE646_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZD78776.1"
                     /translation="SRLEAGTEVVRLERVDLAALVQATLRARGWADRVRVRGGSLEVI
                     SDRRRLERIVSNLVGNAVEHGGAEVEVEVGPDARGVFVSVSDRGPGIRAEDGPRLFDR
                     FFKGDPSRSGGGSGLGLPIALENARLLGGDIEVASHLGAGARFTLRLPSPSSDEGAGI
                     RDRTVTRR"
     gene            568..1485
                     /locus_tag="VE646_01890"
     CDS             568..1485
                     /locus_tag="VE646_01890"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HZD78777.1"
                     /translation="MKRTIVVAVIVLLAASCARTGAGDLGPVAGGETPSAGSSTPTLS
                     PQPPTSPTGAPSAEPTGSGGTAETMTYEVWFTAGEGTLFVTHRTVPADPRIGTAAMES
                     LLAGPTAAERRAGVGSALPSETKLLGLTIQDGLATVDLTGDVESGGGSLLMMLRLGQL
                     TYTLTQFPTVNGVLIELDGRRVDTFSTEGLMTDRPLTRKAYRILLPPILVERPAIGER
                     VSSPLTVVGSADVFEATVSISLLDARGDEIVRTFTTATCGTGCRGTYEKTIRYTVPET
                     QPGILRVYEASAEDGTPVNVVEIPVVLSA"
     gene            1567..3378
                     /locus_tag="VE646_01895"
     CDS             1567..3378
                     /locus_tag="VE646_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZD78778.1"
                     /translation="MDEQAPLWQQRFRAPILQFPAWSRTAPDLLAAISDESGADQVYA
                     WDLATGTRRRVSDEAVGVIHAAVTADGRRAVWFSDPTGDESGTWVSAPFEGGAPEPLL
                     PGAPSGWPEGIAFAPGVVATVLSDRSGFGVYASVGGGPAKELFRDVDSVGIGLSEWEH
                     EGQDRSGLSADGSLLCVSVAQDGDNLHPALVVLDVRTGAEVARQADGPGFGLEALAWS
                     PVPGDQRLAFCHELSDAVRAALWDLGTGERTDVQADLPGQVFPIDWWPDGASLLLLHR
                     LDGRDGLYRCELTSGALREVLPQSGEVHGAGVRPDGRVWFRLSSGSTPPRILDGDGRE
                     VLRPDGPRTPEGRPYRSFHCTSPSGDRVHGFVVVPEGEPPFPMYMKVHGGPSWLYQDT
                     WMRDVQAVVDQGIAVAMVNYRGSTGYGRRFRDLIIGNIGFPEVEDTVAGLDHLIAEGI
                     ADPKRTSIGGWSWGGYTTLLAIGLHPDRFVCAVGGVPVGDYAQSYEESAPELQAYDRS
                     LLGGTVHEVPDLVRERSPITYADRVKTPTLVLVGEHDTRCPPRQALNWVDAVRAAGAE
                     VEVYTFGTGHVSYVVDEEIRQTSRILEFLEKHLLSGG"
     gene            3420..3803
                     /locus_tag="VE646_01900"
     CDS             3420..3803
                     /locus_tag="VE646_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZD78779.1"
                     /translation="MSTVRVRRRGPASKYAIWARYRNLERWPEWAPHIRELRAFGQLR
                     PGLEGQMVGVLGMTAWFEVLEVDEPGGVWIWVVRSGPAKLRIEHEVAEGLAGMIITGR
                     APAVFAYVPVARRQLTRVVGPGRGP"
     gene            complement(3816..4157)
                     /locus_tag="VE646_01905"
     CDS             complement(3816..4157)
                     /locus_tag="VE646_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZD78780.1"
                     /translation="MKKIECYVQPFDFEEVASSLVAAGVTGMSVIEARGFGVQRGMQR
                     GVEPRKGEFLFHPKTKIEIVATDEEVDRVIEAIKTSIKTKQIGEGKIFVTQIEEAVRA
                     RTGEQGEAAIR"
     gene            complement(4285..5646)
                     /gene="gdhA"
                     /locus_tag="VE646_01910"
     CDS             complement(4285..5646)
                     /gene="gdhA"
                     /locus_tag="VE646_01910"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HZD78781.1"
                     /translation="MSGYVDALMSEVTSKNPGEPEFHQAVREVAESLQLVFERHPHYR
                     KEKVLERIIEPERVVLFRVPWVDDAGEVQINKGYRVEMNSAIGPYKGGLRFHPTVYLG
                     MLKFLAFEQVFKNALTTLPMGGGKGGADFDPKGKTDNEVMRFCQSFMTELQRHIGQFT
                     DIPAGDIGVGGREIGYLFGQYKRIRNEFSGVLTGKGLNWGGSLIRPEATGYGAVYFAQ
                     EMLATRDQSLEGKVCLVSGSGNVAQYTLEKILDLGGKPVTVSDSGGFIHDPAGIDREK
                     LAWIMELKNERRCRIREYADAFPGVRFTPADPRADHNPLWDVKADCAFPSATQNEINA
                     KDAANLLNNGVYVVSEGANMPTVPDGVEQFVGAAILYGPGKAANAGGVAVSGLEMVQD
                     SERLSWTREEVDTKLHGIMKAIHEKSRATAEEFGTPGNYVNGANIAGFIKVADAMLDQ
                     GVV"
     gene            5929..7128
                     /locus_tag="VE646_01915"
     CDS             5929..7128
                     /locus_tag="VE646_01915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007515001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZD78782.1"
                     /translation="MPEALIVDAIRTPIGKRGGAYADVHPTRLLSTVLLALFERTGLD
                     PGEVEDHICGCVSQTGEQALNVGRNAWLAAGLPERVPSTTIDRQCGSSQQAAHFAAQG
                     VMAGAYDLVVASGVESMTRVPLGSSLAGGDPFAGGLADRYDGRLVPQGISAELIAHRW
                     RIPREEQDDLGYRSHARAAAATREGRFRGHLVPVQLPGDGSVLLDRDEGIRMEPDRER
                     MRVLEPAFRSPAFDGLFPGQIDWTVTAGNASQISDGATALLVASPPAAERLGLRPRAR
                     FVSFSVVGDDPVFMLTGPIPATRRAMERAGLDLEDLAAVEINEAFASVVLAWKRELGI
                     SDDWFEEHVNPNGGAIAMGHPLGATGGKLMADLVAELERRDGRYGLQVMCEGGGMANA
                     TVLERAP"
     gene            7125..7391
                     /locus_tag="VE646_01920"
     CDS             7125..7391
                     /locus_tag="VE646_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78783.1"
                     /translation="MTWWMVLSAVGMGLAIAWLGLTALRAASGGQPRSRPEVEDVEEL
                     EVFLVCRDCGTEFQVTRLGELQIPRHCGEPMEVVHRPAARGTEG"
     gene            complement(7577..7837)
                     /locus_tag="VE646_01925"
     CDS             complement(7577..7837)
                     /locus_tag="VE646_01925"
                     /inference="COORDINATES: protein motif:HMM:NF018487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HZD78784.1"
                     /translation="MPRSKGRQKQKSSRRPYVPTPQRKKRKSSPRWYGVAILTVMGVG
                     VLMIVLNYIGLMPLTGGQASNLWLWVGLGLIALGFVGATQWR"
     gene            complement(7849..10188)
                     /locus_tag="VE646_01930"
     CDS             complement(7849..10188)
                     /locus_tag="VE646_01930"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HZD78785.1"
                     /translation="MSDWVEQVGEAARAALPPMDGELTVPGLVAPVEVRRDRWGVSHL
                     SAQSEDDLYFAQGFVQASERLFQIDAALRLSNGRLATMFGDLVLPMDRFVRTVGWNRA
                     GARIAAGYDERSARMLTSFILGARAWLEAMAAPPVEYAVLGLEPELPSDPASWAAASV
                     YLAWGLSGNWDEELLRAEILEDLGWDALRDLFPGTAPAAVVAGRMPVAPTAAQLLDSA
                     PVTPRGQGSNNWVVAGGRSQSGSPLLANDPHLPAQAPSVWIEVHLAAPGFEVCGVALP
                     FFPGVVIGRSLRHAWGMTNVGGDTQDLYLERLSEDGAAALYEGAWEPLTVHREEIHVR
                     GREEPEVLEVRETRHGPILDAYLLGLGSPTVVEGGLRHSYALRWVGLDHTVLPSSLHA
                     MAVATSFEEFREALGDWACPGQNFVYADADGAIGYQCTGLHPIRRGSDGTLPVPGWTD
                     RHEWAGFVPFPELPWAADPEDGFLATANSRPYDGSYPHDLGRDFLPPYRTQRIAELLT
                     AIERHTPTTFAAMQSDTISLPARQIAPQLAAVEPASDRQARALGLLRGWDGDLAAGSG
                     AACLYEIWSLHIAREVLLPKLGERLFEHYYARRAGGSLFASPVLAHLLSTPGPRWFGR
                     AGREARDEVLRRALDAALDELETSLGPDPGSWRWGSLHRAVFAGPLAMIQELAELFTA
                     GTVEAGGDGTTLLQGGFEPGGPYHAAVIPSWRMIVDLADPDGGLGVHTTGQSGNPVSP
                     HWNDLVPLWATGDLHPLPMTPAAVDAVTESRLRLLPASR"
     gene            10332..12059
                     /locus_tag="VE646_01935"
     CDS             10332..12059
                     /locus_tag="VE646_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HZD78786.1"
                     /translation="MSEHRPWFDSYEEGVPTTLEPYPDASIFTMLESSARRFPDAPAI
                     AWLGRRLSYARLLREVERFSAVLVSLGVRKGDRVALVMPNCPQYVIGYYACGRIGAIV
                     VGNNPLYTKKEMRLQLKDSGAGVVVVLDLIYIDFAEVFAELGIEQVVVARLNDYLRFP
                     KKQLAPLLRFRKTQREQGKPWPPVPRDAPVRWWHREMKAASPIPPVATVDPVRDAAGF
                     IYTGGTTGVSKGAMLSHRNLVANAFQAAAYISLGEGNESLLCALPFFHSFGMLAMNVG
                     IAKASKLAVVPNARDLHMVLEIMAKEKPTFFPGVPRMFAAINESPLATKLDLRSVRAC
                     VSGGAPLPQAVAKRFAEITGGARLVEGYGLTEASPVTHANPFDGRDRVGSIGLPVPDT
                     ECKIVDLNDPERIVGPGERGELAVRGPQVMLGYWNRPEETARAIINGWLHSGDVAVMD
                     PDGFFHIVDRIKELILVSGFNVYPTEVEEVLYHHPKIAKVCVAGVPDAKTGEAVKAYI
                     VLKPGETATAEEIVAWCRDPQQGLTGYRVPKQVEFRESLPETLIGKVLRRVLQEEERQ
                     RATKAGSPA"
     gene            12056..12781
                     /locus_tag="VE646_01940"
     CDS             12056..12781
                     /locus_tag="VE646_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZD78787.1"
                     /translation="MSPIRAVVFDLFGTLVPEFPAALWKDHFRRMAAVLQVDAGRFED
                     AWRATAVERQTGRLGDIEDNIRVLCELIGHEPSPERVAAAVAVRLDVYAATFHPLPGA
                     EDTLRWLRERGHPIGLISMCAPDTPALWRELPLAPYVDEALFSCQVGLRKPEAEIYRL
                     ACRRLGVAPSESLFVGDGSYGELSGAAAVGMRAVLIRDPAEVEGDIHRPEVEVWPGPS
                     IGALPEVRALLADPVDQPTSSAG"
     gene            complement(12757..13647)
                     /locus_tag="VE646_01945"
     CDS             complement(12757..13647)
                     /locus_tag="VE646_01945"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZD78788.1"
                     /translation="MRLTGAIAAAVTPLRDGGERLDEDAFQPLQRFLADGGLDGILAL
                     GTTGEGIVLSPPERMRVAELFLQDRPHPDFAVAVHCGAQTTADTAALAAHAAEGGADA
                     VAVIAPPYFPLDQEELFQHLRAAAAACEPLPFYVYEFVGRSGYAVPLAVIHRLREAAP
                     NLAGLKVSDTPFEKVEPYLLDGLDVFIGSEPLALQGMERGAVGTVSGLATAWPETVAA
                     LVHDWDPLAHARVVELRERLAGIPFQAALKHVLGLRGVPVGPAVRRPLRTLTDEERAI
                     VKTLEASPIHPPVQPADDVG"
     gene            complement(13713..15089)
                     /locus_tag="VE646_01950"
     CDS             complement(13713..15089)
                     /locus_tag="VE646_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZD78789.1"
                     /translation="MALSSADIARMLVALAMLLVSAHAVGQVFARFRQPRVVGEILGG
                     LVLGPTFLGSLFPAQLSWVFPASGPVAAIIGAVYQLGLLLLMYCSGAEMRSLFHREER
                     RTVAAVTVGGIAVPFLLGLVAVSVIDTSPLEGPAGVHGAFVLVFGIAIAVTSIPVISK
                     ILFDLGIIGTSFSRIVLGAAVVEDVVLYVALAVALAMASATQGGPFGLPSLLGIDPIS
                     RANVVYHVIAEIGFMSAALAIGPSVFGWTRKLRLNLVNASNPMAYQLAFMLMLSGLCA
                     FIGVVPLFGALVAGLVAGASTDEEVIGERTTIKRFSFAFFIPIYFAVVGLQLDLARTF
                     DPLFFAGFLLFATAVKAVSVYVSARLTGETSRGAANLAVAMNARGGPGIVLASVAFDA
                     GIISPSFFASLVMLAVVTSLIAGSWLDRAVRSGMPLRGPGDPAARPQGHIAPGRDRNQ
                     DTSTGSPG"
     gene            complement(15222..16097)
                     /gene="hisG"
                     /locus_tag="VE646_01955"
     CDS             complement(15222..16097)
                     /gene="hisG"
                     /locus_tag="VE646_01955"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZD78790.1"
                     /translation="MLRLVLPKGSLEEQTLQLFQDADLRVRRSSPRDYHGRIEDERIE
                     AVSLLRPQEIPTYVADGLFDLGITGRDWIVETGADVEEIASLEYAKSGTGHGVRIVLA
                     VPNEHPATRARELPSDSRISTEYVNLTRRYFDDLGIPVHVVWSYGATEAKVPQIVDAI
                     VDVTETGSTLRAHGMKIIGTLLESEVLLIANRGSSADPAKRGAMRDVTTLLLGALRAE
                     GRVLIKLNVPADQLSAVLDVVPSMKSPTISQLSDGGYAIETVVDKGSVNTLIPLLKAS
                     GGTDILELPISKIVP"
     gene            complement(16097..16429)
                     /gene="hisI"
                     /locus_tag="VE646_01960"
     CDS             complement(16097..16429)
                     /gene="hisI"
                     /locus_tag="VE646_01960"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HZD78791.1"
                     /translation="MGLLDRTPLDELRFDDRGLIPAIVQDAESGDVLMMAWMSRESLE
                     LTRAEGRTVFWSRSRGELWRKGDTSGHVQHVRDIRADCDADVLLVLVHQVGAACHTGE
                     RSCFFRRV"
     gene            complement(16429..17190)
                     /gene="hisF"
                     /locus_tag="VE646_01965"
     CDS             complement(16429..17190)
                     /gene="hisF"
                     /locus_tag="VE646_01965"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HZD78792.1"
                     /translation="MTLAKRVVPCLDVDSGRVVKGTRFRDLRDAGDPVELAARYDAEG
                     ADELVFLDITATVQGREATLGVVSRTAEQAFIPLTVGGGVRTEQDVRALLRAGADKVS
                     LNTAAVRDPSLLARCAERFGTQCLVAAIDARRRNGGWEVVIEAGRIGTGLDAVAWAAE
                     ATARHGAGEVLVTSMDRDGTGQGYDLELLRAITDAVSVPVVASGGAGRPEHFAQALTE
                     GGADAVLAASRFHFEELSIGQVKAFLRARGIPVRC"
     gene            complement(17187..>17493)
                     /locus_tag="VE646_01970"
     CDS             complement(17187..>17493)
                     /locus_tag="VE646_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HZD78793.1"
                     /translation="RVMVRGWREAGPPVEETLRDLAAAGTPRFLATSVQQDGTMDGPD
                     FALYEHLVGSTEVPIIASGGVRKADDVWRLRELGVEAAVVGKALYSGTMHLSEVVRG"
BASE COUNT         2544 a         6104 c         6301 g         2544 t
ORIGIN      
        1 tcccgcctgg aggccgggac ggaggtcgtc cggctggagc gggtggacct ggcggccctg
       61 gtacaggcca ccctgcgagc ccgggggtgg gccgaccggg tccgggtccg tggcgggagc
      121 ctggaagtga tctcggatcg ccgtcgcctg gagcggatcg tctcgaacct ggtgggcaac
      181 gccgtggagc acgggggggc ggaggtggag gtggaggtgg gccccgatgc tcgaggggtg
      241 ttcgtctccg tctccgatcg tggcccgggc atccgggccg aggacgggcc ccgcctcttc
      301 gatcgcttct tcaagggtga cccctcccgg tccggtgggg gcagcgggct cggcctccct
      361 atcgcgctgg agaacgcccg gctcctggga ggggacatcg aggtcgccag ccacctcggg
      421 gccggagcca ggttcacgct gcggttgccg tcgccatcgt ccgacgaggg cgccgggatc
      481 cgtgaccgaa cggttacacg ccggtgacgc tccggttgcc cccggcagcc acgatggggc
      541 cgatcacgga gagagaggtg accgctcatg aagcgaacca tcgtggttgc ggtgatcgtg
      601 ctcctcgcgg cgtcctgcgc tcgcaccgga gccggcgatc tggggccggt ggccgggggt
      661 gagacgccga gcgccggatc gtccacgccg acgctgagcc cccagccccc gacctcgccc
      721 accggggcgc cctcggccga gccgacgggc agcgggggga ccgccgagac gatgacgtac
      781 gaggtctggt tcacggcggg tgagggcacc ctgttcgtca cccatcggac cgtgccggcc
      841 gacccgcgca tcggaacggc cgccatggag tcgctcctgg ccggcccgac ggcggccgag
      901 cgcagggccg gcgtgggctc cgccctgccc agcgagacca agctcctggg cctcacgatc
      961 caggacggcc tggccacggt ggacctcacc ggcgacgtcg agtcgggggg cggaagcctc
     1021 ctgatgatgc tgcgcctggg tcagctcacc tacacgctga ctcagttccc cacggtgaac
     1081 ggagtgctga tcgagctgga cggccggagg gtggacacct tctccaccga gggactcatg
     1141 accgaccggc cgctgacccg gaaggcgtac cggatcctcc tgcccccgat cctcgtcgag
     1201 cggcccgcca tcggggagcg ggtctcgagc ccgctcacgg tggtcggatc ggccgacgtg
     1261 ttcgaggcca cggtcagcat cagccttctg gacgcccggg gggacgagat cgtccgcacg
     1321 ttcaccacgg cgacgtgcgg aacggggtgc cggggcacgt acgagaagac gatccgctac
     1381 acggtccccg agacccagcc cgggatcctg cgggtctacg aggcctcggc cgaggacggg
     1441 accccggtca acgtggtgga gatcccggtg gtgctgagcg cctgaggctc ggagcggtcg
     1501 atgggcgggc tcgctgggcc cgcccatcgg ggtccgggca tcgggcccgc tgctacgctc
     1561 cgggccatgg acgagcaagc acccctctgg cagcaacggt tccgagcccc gatcctccag
     1621 ttccccgcct ggtccaggac ggccccagac ctgctggcgg ccatctccga cgagtcgggc
     1681 gcggaccagg tctacgcgtg ggatctggcc accggcaccc gtcgacgggt gagcgatgaa
     1741 gcggtcgggg tgatccacgc ggccgtcacg gcggacggcc gtcgcgcggt ctggttcagc
     1801 gaccccaccg gtgacgagtc ggggacgtgg gtctcggcgc cgttcgaggg cggagcgcct
     1861 gagcccctcc tccccggggc cccatcgggc tggcccgaag ggatcgcctt cgcgcccggc
     1921 gtggtggcca cggtgctctc ggaccgaagc gggttcggcg tgtacgcctc ggtcggtggt
     1981 ggaccggcca aagagctgtt ccgggacgtg gactccgtcg ggatcgggct cagcgagtgg
     2041 gagcacgagg gacaggatcg ctcggggctc tcggcggacg ggtcgctgct ctgcgtctcg
     2101 gtcgctcagg acggcgacaa cctccatccc gccctggtgg tgctcgacgt gcgcacgggg
     2161 gcggaggtgg cacggcaggc ggatggtccg gggttcggcc tggaggcgct ggcctggtcg
     2221 ccggtcccag gggaccagcg gctcgccttc tgccacgagc tgagcgacgc cgtgcgggcg
     2281 gccctctggg acctggggac cggggaacgg acggacgtgc aggccgacct gccgggccag
     2341 gtcttcccca tcgactggtg gcccgacggt gcatccctcc tgctccttca ccggctcgac
     2401 ggccgcgacg gcctgtaccg gtgcgagctg accagcggcg cgctcaggga ggtcctgccc
     2461 cagtccgggg aggtgcacgg ggccggcgtg aggcccgacg ggcgggtgtg gttccggctg
     2521 tcgagcggct ccacgccgcc ccggatcctc gacggcgatg ggcgggaggt cctgaggccc
     2581 gacgggccac ggacgccgga gggccgtccc taccggtcct tccactgcac gagcccgtcg
     2641 ggggatcggg tgcacgggtt cgtggtggtg ccggagggtg agccgccgtt cccgatgtac
     2701 atgaaggtcc acggcggacc gagctggctg taccaggaca cgtggatgcg agatgtccag
     2761 gccgtggtgg atcagggcat cgccgtcgcg atggtgaact accgaggctc caccggctac
     2821 gggcgccgct tccgcgacct catcatcggc aacatcggct tccccgaggt ggaggacacg
     2881 gtcgccggcc tggaccacct gatcgccgag ggcatcgccg atccgaagcg gacgtcgatc
     2941 ggcgggtggt cctggggcgg ctacaccacg ctcctggcga tcggcctgca cccggaccgg
     3001 ttcgtgtgcg ccgtgggagg ggtgcccgtg ggggactacg cgcagtccta cgaggagtcg
     3061 gcccccgagc tgcaggcgta cgaccggtcg ctgctgggag ggaccgtgca cgaggtgccg
     3121 gacctggtcc gcgagcgctc ccccatcacc tacgccgatc gcgtgaagac ccccaccttg
     3181 gtgctggtgg gcgagcacga tacccggtgc cctccccggc aggccctgaa ctgggtggac
     3241 gcggtccggg cggccggggc ggaggtcgag gtgtacacct tcggcaccgg gcacgtgtcc
     3301 tacgtggtgg acgaggagat acgacagacc tcgaggatcc tggagttcct ggagaaacac
     3361 ctgctctccg gcgggtagcg atccggacag atcctcgtca catgccggat gatggagcgg
     3421 tgagcacggt tcgggtccgt cgtcgcgggc cggccagcaa gtacgccatc tgggcccggt
     3481 accggaacct cgagcggtgg ccagagtggg ctccccacat ccgcgagctg cgggccttcg
     3541 gtcagttgcg gccgggcctg gagggccaga tggtgggggt cctggggatg accgcctggt
     3601 tcgaggtgct ggaggtcgac gagccgggcg gcgtgtggat ctgggtggtg cgctcgggtc
     3661 cggccaagct ccggatcgag cacgaggtgg ccgagggact ggccgggatg atcatcaccg
     3721 gtcgagctcc ggccgtgttc gcctacgtgc cggtcgctcg gaggcagctc accagggtgg
     3781 tggggccggg cagggggccc taaggttccc gctgctcagc ggatcgcggc ctcgccctgc
     3841 tccccggtgc gggcccggac cgcctcctcg atctgggtaa cgaagatctt gccctcgccg
     3901 atctgcttgg tcttgatcga ggtcttgatg gcctcgatca cccggtcgac ctcctcgtcg
     3961 gtggccacga tctcgatctt cgtcttggga tggaagagga actcaccctt cctcggctcc
     4021 acgccgcgct gcatgccccg ctggacgccg aagcccctcg cctcgatcac gctcatgccg
     4081 gtaacgccgg ccgccaccag ggacgaggcg acctcctcga agtcgaaggg ctgcacgtag
     4141 cattcgatct tcttcacgcc gcgctccttc cactcccccc gccgggcccg gcgcccgcgc
     4201 tgtccctcca actgtacgga gccggtcaag gcccggcagg gcggtgggcc gcccgtgggc
     4261 ggcccaccga ggcgaggggg gagctcagac cacgccctgg tcgagcatgg cgtccgcgac
     4321 cttgatgaag ccggcgatgt tcgcgccgtt cacgtagttc ccgggcgttc cgaactcctc
     4381 ggccgtggcc cggctcttct cgtggatcgc cttcatgatc ccgtgcagct tggtgtcgac
     4441 ctcttcacgg gtccacgaga ggcgctcgct gtcctggacc atctccagtc cggagaccgc
     4501 cacgccgccg gcgttggcgg ccttgccggg accgtacagg atcgccgccc cgacgaactg
     4561 ctccacgccg tcggggaccg tcggcatgtt ggcgccctcg ctgaccacgt agacgccgtt
     4621 gttcaggagg ttcgccgcgt ccttggcgtt gatctcgttc tgcgtggcgc tggggaacgc
     4681 gcagtcggcc ttgacgtccc acagcgggtt gtgatccgcg cgggggtccg ccggcgtgaa
     4741 cctcacgccc gggaacgcgt ccgcgtactc cctgatccgg caccggcgct cgttcttcag
     4801 ctccatgatc caggccagct tctcgcggtc gatcccggcc gggtcgtgga tgaagccgcc
     4861 ggagtccgac accgtgaccg gcttgccgcc gaggtcgagg atcttctcca gcgtgtactg
     4921 cgccacgttc ccggacccgg agaccaggca gaccttgccc tccagcgact gatcgcgcgt
     4981 agcgagcatc tcctgagcga agtagacggc cccgtagccc gtggcctccg ggcggatcag
     5041 cgacccgccc cagttcagcc ccttgcccgt cagcacgccg ctgaactcgt tgcggatccg
     5101 cttgtactgg ccgaacaggt agccgatctc gcgcccgccg accccgatgt cgccggcggg
     5161 gatgtcggtg aactgcccga tgtgccgctg cagctcggtc atgaaggact ggcagaaccg
     5221 catgacctcg ttgtcggtct tgcccttggg gtcgaagtcg gcaccgccct tgccgccgcc
     5281 catcggcagg gtggtgaggg cattcttgaa gacctgctcg aacgccagga acttcagcat
     5341 gcccaggtac accgtggggt ggaagcgcag gccgcccttg tagggaccga tcgcgctgtt
     5401 catctccacc cggtagccct tgttgatctg gacctcgccc gcatcatcga cccagggcac
     5461 gcggaacaag acgacgcgct ccggctcgat gatccgctcg aggaccttct ccttgcggta
     5521 gtgggggtga cgctcgaaga ccagttggag cgattccgcc acctcccgga ccgcctggtg
     5581 gaactcgggc tcgccggggt tcttgctggt gacctccgac atcaaggcgt ctacgtaccc
     5641 gctcatctgc cctcctttcg cgacggatcc gctccggaac gaagcatccc ggcgaggatc
     5701 gccgggtggg agctctcctg accgatggtg cagatcacag cagcatcgtc ccatcccgaa
     5761 ggtatcggcg gcgggccgga tggcccaaac gtcccgggcc ggatggacgg gccggccgtc
     5821 gccacctccg ctggaaccat acccgccgtt cggcgctgca cctcgcagct cgggcgcgga
     5881 ccggccgggc gcgtagactc ggatcggagc aggtcaggag ccgatcccat gcccgaagcc
     5941 ctcatcgtgg acgcgatccg cacccccatc ggcaagcgcg ggggcgccta cgcggacgtg
     6001 caccccaccc ggctgctgtc cacggttctc ctggcgctgt tcgagcgcac cggcctggac
     6061 cccggcgagg tcgaggacca catctgcggc tgcgtgtcgc agaccgggga gcaggccctg
     6121 aacgtggggc ggaacgcctg gctcgcggcc ggcctgccag agcgggtgcc ctcgaccacc
     6181 atcgaccgcc agtgcggctc ctctcagcag gcggcgcact tcgcggcgca gggcgtgatg
     6241 gcgggtgcct acgacctcgt ggtggcctcc ggcgtcgaga gcatgacccg tgtgccgctg
     6301 gggtcctcgc tggccggagg cgatcccttc gccggggggt tggcggaccg ctacgacggc
     6361 cgcttggtgc cccaggggat ctccgcggag ctgatcgccc accgctggcg gatccctcgg
     6421 gaggagcagg acgatctggg ctaccggtcc cacgcccgtg ccgccgctgc cacccgtgag
     6481 gggcggttcc gagggcacct ggtgccggtg cagctgcccg gggacggatc ggtgttgctg
     6541 gatcgggacg aggggatccg gatggagccc gatcgggagc ggatgcgcgt cctggagccg
     6601 gcgttcaggt cgccggcgtt cgacgggctg ttccccgggc agatcgactg gaccgtgacg
     6661 gccgggaacg cctcccagat ctcggacggc gccaccgcgt tgctggttgc ctctcccccc
     6721 gctgcggagc gcctgggcct gagaccccga gctcggttcg tgtccttctc cgtggtgggg
     6781 gacgacccgg tgttcatgct caccggcccc atccccgcca cccggcgggc catggagcgg
     6841 gccggtttgg atctggagga cctggccgcg gtggagatca acgaggcctt cgcctcggtc
     6901 gtcctggcct ggaagcgcga gctggggatc tcggatgact ggttcgagga gcacgtgaac
     6961 cccaacgggg gagcgatcgc catggggcat ccgctcggag ccaccggggg caagctcatg
     7021 gccgacctgg tggctgagct ggaacggcgg gacggtcggt acggcctcca ggtgatgtgc
     7081 gaggggggag gcatggccaa cgcgaccgtg ctggagcggg cgccgtgacc tggtggatgg
     7141 tgctgagcgc cgtcgggatg gggctggcga tcgcctggct cgggctcacg gcactgcggg
     7201 cggcctccgg gggccagcct cgatcccgcc ccgaggtgga ggatgtggag gagctggagg
     7261 tgttcctggt gtgccgggat tgcgggacgg agttccaagt gaccaggctg ggtgagctgc
     7321 agatccctcg gcactgcggg gagccgatgg aggtcgtgca ccgccccgcc gcccggggca
     7381 ccgagggctg agctcccggc gaaccgcgtt ttccccagcg tttccccatc ccggtgggca
     7441 cggaaccccc ggctttcccc aatcggagcc caccccggcg aagagcgcgc tccgcgttcg
     7501 aatgacagcg atgtggtttt ccccacgtgg ggacgtcgtg tggaagatca tcccgccgtg
     7561 atcatcggcg ctcgggtcag cgccactgcg tggcgcccac gaacccgagc gcgatcagtc
     7621 ccaggcccac ccacagccac aggttggagg cttgcccacc ggtcagcggc atgaggccga
     7681 tgtagttcag cacgatcatc agcacgccga cccccatgac cgtgaggatc gcgacgccgt
     7741 accagcgcgg gctcgacttg cgcttcttgc gttggggcgt ggggacgtac ggtcggcgac
     7801 tcgacttctg cttctgacgt cccttcgatc gaggcatacc gggtcaggtt accgcgatgc
     7861 cgggagcagc ctcagccgtg actcggtcac ggcgtcgacg gccgccgggg tcatgggcaa
     7921 cggatgcagg tccccggtcg cccagagcgg cacgaggtcg ttccagtggg gcgagaccgg
     7981 gttccccgac tgtccggtgg tatggacgcc gagccctcca tccggatctg cgaggtccac
     8041 gatcatccgc caggagggga tcacggccgc gtggtagggc ccgccgggct cgaagccgcc
     8101 ctgcaggagc gtggtgccgt ccccgcccgc ctccaccgtt cccgccgtga agagctcggc
     8161 cagctcctgg atcatggcca agggccccgc gaacaccgcc cggtgcagcg acccccagcg
     8221 ccaggacccc ggatcggggc cgagggatgt ctcgagctcg tcgagggcgg cgtccagcgc
     8281 tcggcgcagc acctcgtcgc gggcctcccg gccggcccgg ccgaaccacc gcgggccggg
     8341 tgtggacagc aggtgggcca gcacgggcga ggcgaacagg ctccccccgg cccgccgagc
     8401 gtagtagtgc tcgaacaggc gctccccgag cttgggcagc aggacctccc tcgcgatgtg
     8461 gaggctccag atctcgtaga ggcacgcggc ccccgaaccg gcggcgaggt cgccgtccca
     8521 gccacgcagg agcccgaggg ctcgggcctg ccgatcggat gccggctcca ccgccgcgag
     8581 ctggggggcg atctggcggg cgggcaggga gatggtgtcg gactgcatcg cggcgaaggt
     8641 ggtcggggtg tggcgctcga tcgcggtcag gagctccgcg atgcgctggg tgcggtacgg
     8701 gggcaggaag tcccggccca ggtcgtgcgg gtaggacccg tcgtaggggc ggctgttcgc
     8761 cgtggccagg aacccatcct cggggtcggc ggcccagggc agctccggga acgggacgaa
     8821 ccccgcccac tcatgccggt cggtccatcc cgggacgggg agggtcccgt cgctcccccg
     8881 gcggatcgga tgcagcccgg tgcactggta gccgatcgcg ccgtcggcgt cggcgtagac
     8941 gaagttctga ccggggcagg cccaatcccc gagtgcctcc cggaactcct cgaacgacgt
     9001 ggccacggcc atcgcgtgca ggctggacgg cagcaccgtg tggtccagcc ccacccatcg
     9061 cagggcgtag ctgtgccgca gcccgccctc caccaccgtg ggagagccca ggccgagcag
     9121 gtaggcgtcc aggatcgggc cgtggcgcgt ctcacggacc tcgagcacct ccggctcctc
     9181 gcggccccgc acgtggatct cctcccggtg caccgtgagc ggctcccagg cgccctcgta
     9241 cagcgcggcc gcgccgtcct cgctcaaccg ctccaggtag agatcctgcg tgtccccgcc
     9301 gacgttggtc atgccccacg cgtgccggag cgagcgcccg atcaccaccc ccgggaagaa
     9361 ggggagggcg acgccgcaga cctcgaaccc gggcgccgcc aggtgtacct cgatccacac
     9421 cgacggcgcc tgggccggca ggtggggatc gttggccagg agcggcgagc cgctctggct
     9481 ccggcccccc gcgaccaccc agttgttcga cccctgcccc cggggcgtga ccggcgccga
     9541 atcgagcaac tgagccgcgg tgggcgccac gggcatcctg ccggccacga cggcagcggg
     9601 agcggtgccc gggaagaggt cgcggagcgc gtcccacccc aggtcctcaa ggatctcggc
     9661 ccgcagcagc tcctcgtccc agttcccgga gagtccccat gcgaggtaga cgctggcggc
     9721 ggcccacgag gccgggtccg aaggcagctc gggctcgaga ccgagcacgg cgtactcgac
     9781 gggaggcgcg gccatggcct ccagccaggc ccgagcgccc aggatgaagg aggtgagcat
     9841 gcgtgcggat cgctcgtcgt acccggcggc gatgcgggcc ccggcgcggt tccatcccac
     9901 ggtgcggacg aagcggtcca tgggcagcac caggtcgccg aacatcgtgg cgaggcggcc
     9961 gttggacagg cggagcgcgg catcgatctg gaacagacgc tccgaggcct ggacgaagcc
    10021 ctgagcgaag tacaggtcgt cctcgctctg ggcggacagg tgggagacgc cccaccggtc
    10081 gcgccggacc tccaccgggg ccacgagccc ggggacggtg agctccccgt ccatgggcgg
    10141 cagcgcggcc cgagcggcct cgcccacctg ctcgacccag tcgctcacgg cagaggcaac
    10201 cctacctgcc ggggcggtgc ggcggcgcac gcccgcggcg ccctcccacg gcgaccggtt
    10261 cgaaggtccg taccggtccg gagccaccgg acgttgagcg gtccccggtc gctctggaat
    10321 gattcctcta cgtgagcgag catcggccct ggttcgacag ctacgaggag ggcgtcccca
    10381 cgaccctgga gccctacccc gacgcgtcca tcttcacgat gctcgagtcc tcggcccgcc
    10441 ggttcccgga cgctccggcc atcgcgtggc tgggccgccg gctctcctac gcgcggctgc
    10501 tgcgtgaggt ggagcggttc tcggcggtcc tggtctcgct gggggtccgc aagggagacc
    10561 gggtcgcgct cgtgatgccc aactgtcccc agtacgtcat cggctactac gcgtgcggcc
    10621 ggatcggggc gatcgtggtg ggcaacaacc ctctgtacac caagaaggag atgcgcctcc
    10681 aactgaagga ctccggcgcc ggggtggtcg tggtcctgga cctgatctac atcgacttcg
    10741 cggaggtctt cgccgagctg ggcatcgagc aggtggtggt ggccaggctg aacgactacc
    10801 tgaggttccc caagaagcag cttgcgccgc tgctgcggtt ccgcaagacg cagcgggagc
    10861 agggcaagcc ctggccgccc gtgccccggg acgccccggt ccgctggtgg caccgggaga
    10921 tgaaggcggc ctcgccgatc cctccggtgg ccacggtgga cccggtccgt gacgcggcgg
    10981 gcttcatcta cacgggaggc acgaccggcg tgtcgaaggg ggccatgctc tcccaccgca
    11041 acctcgtggc caacgcgttc caggccgccg cctacatctc gctgggtgag ggcaacgaga
    11101 gtctcctgtg cgcgctgccg ttcttccatt ccttcgggat gctggcgatg aacgtgggga
    11161 tcgcgaaggc ctccaagctc gcggtggtcc cgaacgcgcg agacctgcac atggtcctgg
    11221 agatcatggc caaggagaag cccacgttct tcccgggggt cccgcggatg ttcgccgcta
    11281 tcaacgaatc gccgctggcc accaagctcg acctccgctc ggtgcgagcc tgcgtctccg
    11341 gcggggcgcc cctgccccag gccgtggcca agcgcttcgc cgagatcacc ggcggagccc
    11401 gtctggtgga ggggtacggg ctgactgagg cctcccccgt gacccacgcc aatcccttcg
    11461 acgggcggga tcgagtgggc tcgatcggcc tgccggtccc cgataccgag tgcaagatcg
    11521 tggacctgaa cgatccggag cggatcgtgg gtcccgggga gcggggagag ctggcggtcc
    11581 ggggccccca ggtgatgctc ggctactgga accgtcccga ggagaccgct cgggccatca
    11641 tcaatgggtg gctgcattcc ggtgacgtgg cggtcatgga cccggacggg ttcttccaca
    11701 tcgtggaccg gatcaaggag ctgatcctgg tctccgggtt caacgtgtat cccaccgagg
    11761 tggaggaggt cctctaccac caccccaaga tcgccaaggt ctgcgtggcg ggggtgcccg
    11821 atgcgaagac cggtgaggcc gtcaaggcct acatcgtcct gaagccgggc gagacggcga
    11881 ccgccgagga gatcgtggcc tggtgccgcg atccccagca gggactgacc ggctaccgcg
    11941 tccccaaaca ggtcgagttc cgcgagtcgc tgcccgagac gctgatcggc aaggtgctgc
    12001 ggcgggtgct gcaggaggag gagcgtcagc gagccacgaa ggccggatcg ccggcgtgag
    12061 cccgatccga gcggtggtgt tcgacctgtt cggcacgctg gtgccggagt tcccggcggc
    12121 gctgtggaag gaccacttcc gccggatggc ggcggtcctg caagtcgacg ccggtcgctt
    12181 cgaggatgcc tggcgtgcga cggcggtgga gcggcagacg gggcggttgg gcgatatcga
    12241 ggacaacatc cgcgtactgt gcgagctgat cggccacgag ccgagccccg agcgggtggc
    12301 cgcggcggtg gccgtgcggc tggacgtgta cgcggccacc ttccacccgc tgccgggggc
    12361 cgaggacacg ctgcggtggc tccgcgaacg gggtcatccg atcgggctga tcagcatgtg
    12421 cgcgccggat acgccggcgc tgtggcgtga gctgccgctc gcgccgtacg tggacgaggc
    12481 cctgttctcg tgccaggtgg gcctgcgcaa gcccgaggcg gagatctacc gcctggcctg
    12541 ccggcgcctc ggggtcgctc cctccgagag cctgttcgtg ggggacggga gctacggcga
    12601 gctctcgggg gccgccgcgg tgggcatgcg ggcggtgttg atccgtgatc ccgccgaggt
    12661 ggagggggac atccaccgcc ccgaggtgga ggtgtggccg gggccctcga tcggagcgct
    12721 gcccgaggtc cgagcgctgc tcgccgaccc ggtcgatcag cccacgtcgt cagccggctg
    12781 aacgggcggg tggatcgggc tcgcctcgag cgtcttcacg atcgctcgct cctcgtcggt
    12841 gagggtccgg agcgggcgac ggacggcggg acccaccggc accccgcgca gccccaggac
    12901 gtgcttcagg gcggcctgga aggggatccc ggccagccgc tcgcggagct cgaccactcg
    12961 ggcgtgggcc aggggatccc agtcgtgcac cagggcggcc acggtctcgg gccaggcggt
    13021 ggccaggccg gagaccgtcc ccaccgcgcc ccgctccatg ccctgcagcg cgagcggctc
    13081 tgaaccgatg aacacgtcca gtccgtccag caggtagggt tccaccttct cgaacggtgt
    13141 gtccgagacc ttgaggccgg ccaggttcgg ggcggcctca cggagccggt ggatcaccgc
    13201 gagggggacc gcgtagccgc ttcgtccgac gaactcgtag acgtagaagg ggaggggttc
    13261 gcaggcggcg gcggcggccc ggaggtgctg gaagagctcc tcctgatcca gcgggaagta
    13321 cggcggagcg atcacggcca ccgcgtccgc tcccccctcg gccgcgtggg cggccagggc
    13381 ggccgtgtcg gcggtggtct gcgctccgca gtgcacggcc accgcgaagt ccggatgcgg
    13441 ccggtcctgg aggaacagct cggccacgcg catccgctcg gggggcgaca gaacgatccc
    13501 ctcccccgtg gtgccgagcg ccaggatgcc gtccaggccg ccgtcggcca ggaaccgctg
    13561 caacggctgg aacgcatcct cgtccaggcg ctctcctcca tcccgcagag gcgtcacggc
    13621 cgcggcgatc gctccggtca gtcgcacggg ctggactcta ccggagctcg tcagggcccg
    13681 acccgcagcg gcgagccgcg ggatgcctcc catcacccgg gcgatccggt cgacgtatcc
    13741 tggttccgat cccggcctgg tgcgatatgg ccctgtggcc gagcggccgg gtcccctggc
    13801 ccccggagcg gcatcccgct ccggaccgcg cgatcgagcc agctgccggc gatcagcgag
    13861 gtcacgaccg ccagcatgac cagggaggcg aagaaggaag gactgatgat ccccgcatcg
    13921 aacgcgacgg aagccaggac gatcccagga ccgccgcgcg cgttcatcgc cacggcgagg
    13981 ttggccgccc cgcggctcgt ctcaccggtc agtcgagcgc tgacgtacac gctgaccgcc
    14041 ttcacggcgg tcgcgaacag caggaagccg gcgaagaaca gcggatcgaa ggtccgagcc
    14101 aggtccagct gaagaccgac aaccgcgaag tagatgggga tgaagaacgc gaacgagaag
    14161 cgtttgatgg tcgtgcgctc gccgatgacc tcctcgtcgg tggatgcccc ggccacgagg
    14221 cccgcaacga gcgcaccgaa cagaggtacc accccgatga aggcgcacag ccccgagagc
    14281 atcaacatga acgccagctg gtaggccatc gggttgctgg cgttcacgag gttgagccgc
    14341 agcttcctgg tccatccgaa cacgctgggt ccgatcgcga gcgctgcgct catgaacccg
    14401 atctcggcga tgacgtggta gacgacgttg gcgcgcgaga tcgggtcgat gccgaggagg
    14461 gagggaaggc cgaacggtcc cccctgcgtc gcgctcgcca tggccagtgc gacggcgagc
    14521 gccacgtaca gcacgacgtc ctccaccacg gcggcgccga ggacgatcct ggagaaggag
    14581 gtgccgatga tccccagatc gaagaggatc ttggagatca ccgggatgct ggtcaccgcg
    14641 atggcgatcc cgaacacgag gacgaaggcg ccgtgcacgc cggccggacc ctccagcggg
    14701 ctggtgtcga tcaccgagac ggccaccaga cccagcagga aggggacggc gatcccgccg
    14761 accgtgaccg ccgcgacggt cctgcgctcc tcccgatgga agagcgaacg catctcggcg
    14821 ccggagcagt acatcagcag gaggagcccc agctgataga cggcgccgat gatggcggcg
    14881 accgggcccg acgccgggaa gacccacgac agctgcgccg ggaacagcga tcccaggaag
    14941 gtgggtccca ggacgagacc tccaaggatc tcaccgacca cccgcggctg gcggaaccgg
    15001 gcgaagacct gtcccacggc gtgcgccgac accagcagca tcgccagggc cacgagcatg
    15061 cgtgcgatat ctgcggacga aagcgccatc caccctccct ctgtgggtcc ggcacacgct
    15121 acgaacgcgg ccggttggtg cccaccgccg tacgccgtag gtcacgtgcg ccgcgtgtcg
    15181 tatggggacg ggccggtgga ccggcagcga ccgtccgcac ctcaggggac gatcttggag
    15241 atcggcagct ccaggatgtc ggtgccaccg ctcgccttga gcaaagggat cagggtgttc
    15301 acgctgccct tgtccacgac cgtctcgatc gcgtacccgc catcggagag ctgggagatc
    15361 gtcggcgact tcatcgaggg caccacgtcc aacaccgcgc tgagctggtc ggcgggcacg
    15421 ttcagcttga tcagcacccg gccctcggcg cggagggccc cgagcaacag cgtggtcacg
    15481 tcccgcatcg ccccgcgctt ggcgggatcg gcggacgagc cccggttcgc gatcagcagg
    15541 acctcggact cgagcagcgt gccgatgatc ttcattccgt gcgcccgcaa cgtggagccg
    15601 gtctcggtga catcgacgat cgcgtcgacg atctgcggca ccttcgcctc ggtcgccccg
    15661 tacgaccaga ccacgtgcac cgggatcccc agatcgtcga agtaccgccg cgtgaggttc
    15721 acgtactcgg tggagatcct ggagtccgaa gggagctccc tcgccctggt cgccgggtgc
    15781 tcgttcggga ccgcgaggac gatccggacc ccgtgcccgg tgccgctctt cgcgtactcc
    15841 agcgaggcga tctcctccac gtccgccccg gtctccacga tccagtcccg cccggtgatc
    15901 ccgaggtcga acaggccgtc ggccacgtag gtggggatct cctgggggcg gaggagggag
    15961 acggcctcga tccgctcgtc ctcgatccgg ccgtggtaat cgcggggcga gctccgacgc
    16021 acccggaggt ccgcgtcctg gaacagttgg agcgtctgct cctcgagcga ccccttgggc
    16081 aggacgagcc gcagcatcac accctccgga agaagcacga gcgttcgccc gtgtggcagg
    16141 cggcgcccac ctgatggacc aggaccagga gcacgtcggc atcgcagtcg gcgcggatgt
    16201 cccgcacgtg ctggacgtga ccggaggtgt cgcccttgcg ccagagctcg ccacgggaac
    16261 ggctccagaa caccgtgcgc ccctcggccc gcgtgagttc cagggactcc cggctcatcc
    16321 acgccatcat caggacatcg cccgactcgg cgtcctggac gatggccggg atcagtcccc
    16381 gatcgtcgaa ccggagctcg tcgaggggcg tgcggtccag gagccccatc agcacctcac
    16441 cgggatccct cgcgcccgga ggaaggcctt cacctggccg atcgacagct cctcgaagtg
    16501 gaatcggctg gcggccagca cggcgtcggc gccaccctcg gtgagggcct gcgcgaagtg
    16561 ctcggggcgg ccggccccgc cgctcgccac gaccggaacg ctcacggcat ccgtgatcgc
    16621 ccgcagcagc tccaggtcgt agccctgccc ggtgccgtcc cggtccatcg acgtcacgag
    16681 cacctcaccg gcgccgtggc gcgccgtggc ctcggccgcc caggccacgg cgtccaggcc
    16741 cgtcccgatc cgcccggctt cgatcaccac ctcccagccc ccgttccggc gccgggcgtc
    16801 gatcgcggcc accaggcact gggtgccgaa ccgctcggcg caccgggcca gcagtgatgg
    16861 gtcgcgcacg gcggctgtgt tcaaggagac cttgtcggcg cccgcgcgga gcagggcccg
    16921 gacgtcctgc tcggtgcgga cccccccgcc caccgtgagc gggatgaacg cctgctcggc
    16981 ggtccgcgag accacgccga gcgttgcctc ccggccctgc accgtggcgg tgatgtccag
    17041 gaagaccagc tcgtccgcgc cctcggcgtc gtagcgggcc gcgagctcca cggggtcgcc
    17101 cgcgtcgcgc aggtcgcgga acctggtccc cttgaccacc cgaccggagt cgacgtccag
    17161 acaggggacg acgcgcttgg cgagcgtcat ccccggacca cctcggacag gtgcatcgtg
    17221 cccgagtaca gggccttgcc caccacggcg gcctccacgc cgagctcgcg caggcgccag
    17281 acgtcgtcgg ccttgcgcac gcctccgctg gcgatgatgg ggacctccgt gctccccacc
    17341 aggtgctcgt acagggcgaa gtccgggccg tccatggtgc cgtcctgctg gacggacgtg
    17401 gccaggaagc gcggggtgcc ggcggccgcc aggtccctca gcgtctcctc gaccggcgga
    17461 cccgcctccc gccagcccct gaccatgacc cga
//
LOCUS       DASQQQ010000040        11546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_251267, whole genome shotgun sequence.
ACCESSION   DASQQQ010000040 DASQQQ010000000
VERSION     DASQQQ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11546
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251267"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2036)
                     /gene="dnaE"
                     /locus_tag="VE646_01975"
     CDS             complement(<1..2036)
                     /gene="dnaE"
                     /locus_tag="VE646_01975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZD78794.1"
                     /translation="MSGRSFVHLHTHTEYSLLDGASRIPALFQAAGEMGMPALAITDH
                     GAMFGALGFYEAGLAAGIKPIIGVEAYVAPGSRFERSPGESEEKYRHLTIVAASERGY
                     RNLLQLVTDAHLEGFYHRPRIDKQLLAEHAEGLIGMSGCLASEISQLLLTGQQDRAVE
                     VASTYGDIFGPGNFYVELQDHGLPDQRTILPGLIDLAHRSRLPMVATNDLHYTFRRDA
                     KPHDVLLCIQQQKVQTDTNRLKFDTDEFYLKSPQEMREVFREMPQACDATLEIAERCE
                     LELVYGDRAPADQRFHLPRFDVPESMRLEEYLRRLVLEGARERYGELTSEILERIDHE
                     LRIITAMGFAGYFLIVWDLIRFARDNGIRVGPGRGSAAGSVVSYSLRITDLDPLRYGL
                     IFERFLNPERKQMPDIDMDFDDRRRDEVIRYVAQKYGSDHVAQIITFQTIKGKQGIRD
                     AARVLGFPASTGDRYCKMYPAAVLGREYPIERALDLSPELRAAYDQEPEAREIIDTAQ
                     ALEGLRREDSVHAAGVVIGDAPLVNYLPLKLAKDSRDDSRRIVTQFDMEGVEKLGLLK
                     MDFLGLRNLSVIEETLALLRRRGIELDIDHVPLEDEAAYQMLRAADTTGVFQLEGGGM
                     RSLIRDLAPDRFEDIMALNALYRPGPLSQGMHTEYAARKHGRKQVEYPHPDLEQ"
     gene            2183..3310
                     /locus_tag="VE646_01980"
     CDS             2183..3310
                     /locus_tag="VE646_01980"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZD78795.1"
                     /translation="MRLSYSSINTYETCPAKFRFQYEEHLPSGRSPALSFGDSLHRAL
                     HRFHDRPVPVAPPLEELHEILDAVWVSEGFRDASEEQMYRDHGRQVMTQYHHENAPHY
                     RIPAAMEFRFLIEVEGVQLSGVIDRMDRIPGGGYEIIDYKTNRRLPPQAAIDRDLQLS
                     IYHLAAKQVWGIEPERLTLYYLLPGQRMSTTRSARDVDQLRRRLATVAERIEAGRFEP
                     RQNPLCDWCDFQRLCPLFRHKYEREEGDPAPRMTQIVDEWIALKRQGREIYRRLDELA
                     GDIHAFCDEHGYRRLFGSDGAAVDRRPQHVSEPDEGRLRRILEPLGLWERVRSVDPQK
                     LSDLIESRTLPPDVEDAVLASRHEVRTQHALYLKNGAAARR"
     gene            complement(3323..4216)
                     /locus_tag="VE646_01985"
     CDS             complement(3323..4216)
                     /locus_tag="VE646_01985"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZD78796.1"
                     /translation="MGARYVALRGRLDMVAAELSGLPRAEVQRAIAAGRVRVDGRSRP
                     KSFSLLGGEVLELDLAGMVELPAEGPAVELAYRDPDLVVVSKPPGLPTHPTANRRSGT
                     LVNRLLGMGVPLADTGDPLRPGIVHRLDAGTSGLMVVACSEAAAPLLVAMFRRHEVGR
                     RYLALVRGVVEHERFAVEAPLGRRGARIRVQATGARPAETRFDVLERFPRVTLLEATP
                     RTGRTHQIRVHLSSVGHPILGDRRYGGGGQDAERLGLSRPFLHSHRLAFEHPLSGRRI
                     RTQDPLPEDLERALRRVRGEG"
     gene            complement(4221..4676)
                     /gene="lspA"
                     /locus_tag="VE646_01990"
     CDS             complement(4221..4676)
                     /gene="lspA"
                     /locus_tag="VE646_01990"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZD78797.1"
                     /translation="MWLYLGAALVYALDRGTKLWAEHALHGRPPITLIPDVLRLTYTT
                     NSGGAFGLGQSAPWIFATATFLVAIAIIVASARLSRLATALGLGSILGGALGNLTDRL
                     VHGHGFLAGRVIDFIDFHVWPVFNVADASIVVGALFLLLVGLRDEREER"
     gene            complement(4711..5100)
                     /locus_tag="VE646_01995"
     CDS             complement(4711..5100)
                     /locus_tag="VE646_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HZD78798.1"
                     /translation="MAVKSPYTKKELADLRERLVEEKEQLSAQLQGIEEATFANSQSD
                     ISGEVSLDDENADAGTFTFERERDLSIENNVRDLLAKIDRALGRMDEGTYGLCSRCGK
                     PIEKARLKALPYVDLCIKDAQSQSRPR"
     gene            complement(5150..5413)
                     /locus_tag="VE646_02000"
     CDS             complement(5150..5413)
                     /locus_tag="VE646_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HZD78799.1"
                     /translation="MAVVTVRVVPRSGRTAVEVEPSRGVVVRVRSAPEAGRATAEALR
                     ALASAIGIAPSRIRLRSGTRSRTKVYAVSGLTQEELDVRLSAT"
     gene            5730..8882
                     /gene="ileS"
                     /locus_tag="VE646_02005"
     CDS             5730..8882
                     /gene="ileS"
                     /locus_tag="VE646_02005"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018848746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HZD78800.1"
                     /translation="MRRFEPVDARAGFPELERDILAFWREADVFHRSMQQRAGAPEWV
                     FYEGPPTANGKPGLHHTESRTFKDVYPRFKTMTGHSVPRKGGWDCHGLPVELEVEKEI
                     GTKTKRDIEAFGIGEFNRLCRASVTRYVDEWERLTERLGFWIDTADAYWTMNTEYVES
                     VWWSLKELHRKGLLFEADKVTTYCPRCGTTLSDHETALGYTQVTDPSVFIRFQIVDSP
                     VPELIGASLLGWTTTPWTLISNTGLGVAADARYVVVEHEGAHLVLAEALRERVLPDAP
                     VVGPAVTGAALVGTRYRPLYPNVEGAHRVADAEFVSLEDGTGVVHMAPAFGAGDMEIG
                     RREGWPVFKPIDGEGRFTDEAPAFVRGLFFKEADAPITEELRARGLLVRAETIDHSYP
                     LCWRCGTPLLYYARNSWYIRTTAVKERLLQVNEEVSWYPAHIKHGRYGDWLENNVDWA
                     LSRERYWGTPLPIWRCPAGHDTAIGSLTELSERAGRDLTAIDPHRPAIDEVTFPCPGC
                     GGTATRVPEVIDTWYDSGAMPFAQWGYHPELGRGKEMFDQRFPADFISEAIDQTRGWF
                     YTLMAEGVLHFDSICYRNVVCLGLIVDKDGRKMSKSVGNVIDPWDVLDRQGADALRWY
                     LITSGSPWAARRVGMEILDEVVRRFLLTWWNVYAFFVLYANASGFDPAEHEVPPSERP
                     LLDRWILSRLSQTVAEAREGLEAYDATGAGRRIAAFVEDLSNWYVRLSRPRFWDPQGR
                     MSVDTVAGFLTLHECLVTLSRLLAPFTPFLAEALWRNLAAGRGDAPDSVHLSDYPRPS
                     GGMVDPLLDRAMADAREVVELGRRIRTEAKVRVRQPLAEAVIQVPGDQGPLRPLLPLI
                     ADELNVRSVTFAGSADRFGSWRAKPDFRALGPRLGPQVKEVASALSEDDGALAARLAG
                     GESVTVRTSTGEVALHPSDVELAQKTVSGWGLASDGSITVALELELTEELRREGLARE
                     LIRAIQDARKAAGLDVTDRIVLGVAADGAVGEALRMHRDVIMSETLATVLEEGSPSHA
                     EHVQEVSVEGSAVTIGLRRD"
     gene            complement(8923..9642)
                     /locus_tag="VE646_02010"
     CDS             complement(8923..9642)
                     /locus_tag="VE646_02010"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HZD78801.1"
                     /translation="MRKKKQEASQHTGFGGDPLSPRLSPEDVQQKEFRLAFRGYNERD
                     VDAFLDHVTEEVGAYQEEARRLRGELEAARGADAGLGNDAAAGQARADAEGILAGARR
                     EAERIVAEARSEAARIAAAAAGGGAGADARAVMAPFLARERGFLQELGQLVQQHAEAV
                     RQMVQAARDGHGAEGPDAGSQEQSTTREGTGPDQPVSVPEAEPDAAVGAAAEGSDEPG
                     TEPPSEAEGGRSLRDLFWGED"
     gene            complement(9658..9951)
                     /locus_tag="VE646_02015"
     CDS             complement(9658..9951)
                     /locus_tag="VE646_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HZD78802.1"
                     /translation="MTAAGNIVLSAVCVLLTVYWFILLAQVLLSWAFVLGVRRPYSGP
                     GRVVMDALDAVTYPVLRPLRSLIPPVRMGGMGLDLSVLLAFVILWVLRVALGC"
     gene            complement(9970..10446)
                     /gene="sepF"
                     /locus_tag="VE646_02020"
     CDS             complement(9970..10446)
                     /gene="sepF"
                     /locus_tag="VE646_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HZD78803.1"
                     /translation="MPGVWKKTLTYLGLVEDDEFEDLDEVAAPESAPVRRLQRPRAVR
                     EVLVEPEDQGIVRTIPATRAAMASSIHKSEPRRFNEAREVADRFKGGIPVIMNLQSTD
                     DTIARRLVDFASGLVYGLDGKIEMVANRVYLLTPANMEVSAEDRERIAGGDFYNQF"
     gene            complement(10456..11151)
                     /locus_tag="VE646_02025"
     CDS             complement(10456..11151)
                     /locus_tag="VE646_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HZD78804.1"
                     /translation="MVLRSRDAIGDNVARIIERIAEACRRAGRERSEVRLVGASKTVG
                     PEAIGWAFESGVREFGENYVGELRDKRGAVPDATWHFIGTLQSHTVHHVADLADVVQT
                     LAPGSAVRRLGRRAVRGGRRLVALIEVDFTGARTGVAPERASAFVDEVASVEGLQLVG
                     LMTMPPFFSEPEEARPFFRRLRELRDRLARDHPSLTELSMGMSMDFPVAIEEGATMVR
                     IGTALFGERPPTT"
     gene            complement(11218..>11546)
                     /locus_tag="VE646_02030"
     CDS             complement(11218..>11546)
                     /locus_tag="VE646_02030"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HZD78805.1"
                     /translation="MQRAASLFGARREVRVAVGPAIGPCHYEVGADVALAVAAGSGAG
                     AMTRRRGDRVFLDLVGTTRAVLREQGIRRIWDTGECTACGRRRFFSYRRDGVTGRHMA
                     LGMRLK"
BASE COUNT         1755 a         4142 c         3944 g         1705 t
ORIGIN      
        1 atctgttcca ggtccggatg ggggtactcc acctgcttgc ggccgtgctt gcgggccgca
       61 tactccgtgt gcatcccctg actgagcggc cccggccggt acagagcgtt cagggccatg
      121 atgtcctcga agcggtccgg agccaggtcg cggatcagtg agcgcatccc gccaccctcg
      181 agctggaaca cgcccgtggt atcagcggcc cggagcatct ggtacgcggc ctcgtcctcg
      241 agcgggacgt ggtcgatgtc cagctcgatg cctcggcgcc gcaacagagc gagcgtctcc
      301 tcgatcaccg agaggttccg caagccgagg aagtccatct tgagcagccc gagcttctcc
      361 acgccctcca tgtcgaactg cgtcacgatg cgccgggagt cgtcgcggga gtccttggcc
      421 agcttcaggg gcaggtagtt gaccaggggc gcgtcgccga tcaccacgcc ggccgcgtgc
      481 accgagtcct cccgccgaag cccctccagg gcctgggccg tgtcgatgat ctcccgggcc
      541 tcgggctcct ggtcgtaggc ggcccggagc tcgggcgata ggtccagggc tcgctcgatc
      601 gggtactccc ggccgaggac cgcggccggg tacatcttgc agtatcggtc gcccgtggaa
      661 gccgggaagc ccagcacgcg cgcggcgtcg cggatgcctt gcttgccctt gatggtctgg
      721 aaggtgatga tctgggccac gtggtccgag ccgtacttct gggccacgta ccggatcacc
      781 tcatcgcggc gccgatcgtc gaagtccatg tcgatgtcgg gcatctgctt gcgctcgggg
      841 ttcaggaagc gctcgaagat cagcccgtag cgcagcgggt ccaggtccgt gatccggagg
      901 gagtacgaga ccaccgaccc ggccgccgat ccccgacccg gccccacccg gatgccgttg
      961 tctcgagcga accggatcag gtcccacacg atcaggaagt aaccggcgaa ccccatcgcg
     1021 gtgatgatcc gcagctcatg atcgatccgc tcgaggatct ccgacgtgag ctccccgtag
     1081 cgctcccggg ccccctcgag caccaggcgc cgcaggtact cctccagccg catgctttcc
     1141 ggaacgtcga agcggggcag gtggaaccgc tggtccgccg gcgctcggtc cccgtacacg
     1201 agctcgagct cgcagcgttc cgcgatctcg agggtcgcgt cgcacgcctg gggcatctcc
     1261 cggaacacct cccgcatctc ctgcggcgac ttcaggtaga actcgtcggt gtcgaacttc
     1321 agccggttcg tgtccgtctg gaccttctgc tgctggatgc acagcagcac gtcgtgcggc
     1381 ttggcgtcgc gtcggaacgt gtagtgcaga tcgttggtgg ccaccatcgg gagccgggag
     1441 cggtgcgcca ggtcgatgag ccccggcagg atcgtccgct gatccgggag cccgtgatcc
     1501 tgcagctcca cgtagaagtt gcccggcccg aagatgtcgc cgtaggtgct ggccacctcc
     1561 acggcgcggt cctgctggcc ggtcaagagc agctgggaga tctcggaggc caggcacccc
     1621 gacatcccga tcagcccctc ggcgtgctcg gcgaggagct gcttgtcgat ccggggccga
     1681 tggtagaagc cctccaggtg agcgtcggtg accagctgga gcaggttccg gtacccgcgc
     1741 tcgctcgcgg ccacgatcgt gaggtgacgg tacttctcct cgctctcccc gggcgaccgc
     1801 tcgaaccgcg aaccgggcgc cacgtaggcc tccacaccga tgatgggctt gatcccggcg
     1861 gctagaccgg cctcgtagaa gccgagggca ccgaacatgg cgccgtgatc ggtgatggcc
     1921 agggccggca tgcccatctc ccccgccgcc tggaacaggg ccggaatcct ggacgcgccg
     1981 tccagcagcg agtattcagt gtgcgtgtgc agatggacga agctgcggcc cgacatcact
     2041 ctcgaagcgt ctcgacgatc gtgctgcctc ggcgtgtgca gaacctgtgc acagcctgtg
     2101 gaagcttaca tgcttgtgat tcctgccggc tcaactctat actcgcgggg ttccgtgtca
     2161 aacgtcgagg tgagcgcgac cggtgagact ctcgtatagc agcatcaaca cgtacgagac
     2221 ctgccccgcg aagttccgct tccagtacga ggagcatctg ccctccggcc ggtcccccgc
     2281 gctgtccttc ggcgactccc tccaccgggc gctccaccgg ttccacgacc ggccggtgcc
     2341 cgtggcgccg cccctggagg agttgcacga gatcctcgac gccgtctggg tgagcgaggg
     2401 cttccgggac gcctccgagg agcagatgta ccgggatcac gggcgacagg tgatgaccca
     2461 gtatcaccac gagaacgcgc cccactaccg gatccccgcc gccatggagt tccggttcct
     2521 gatcgaggtg gagggggtgc agctgtccgg cgtgatcgac cgcatggacc ggatccccgg
     2581 cggtgggtac gagatcatcg actacaagac caaccgccgg ctcccccccc aggcggcgat
     2641 cgaccgcgat ctccagctgt cgatctacca cctggctgcc aagcaggtct ggggcatcga
     2701 gccggagcgg ctgaccctct attacctgtt gcccggccag cgcatgtcca ccacccgttc
     2761 ggcgcgcgac gtggaccagc tccggcgacg actcgcgacc gtggccgagc ggatcgaggc
     2821 gggccggttc gagccccgcc agaaccccct gtgcgactgg tgcgacttcc agcgcctgtg
     2881 ccccctgttc cgccacaagt acgaacggga ggagggcgat ccggcgcctc gcatgaccca
     2941 gatcgtggac gagtggatcg ccctgaagcg tcagggccgc gagatctacc ggcgcctgga
     3001 cgagctggcc ggcgacatcc acgccttctg cgacgagcac gggtaccggc ggctgttcgg
     3061 ctccgacggt gcggccgtgg accgccgccc ccagcacgtc agcgagcccg acgagggacg
     3121 tctacggcgg atcctggaac cgctcgggct gtgggagcgg gtccgctcgg tggatcctca
     3181 gaagctgagc gatctgatcg agtcccgcac gcttcccccg gatgtggagg acgccgtgct
     3241 ggcctcgcgc cacgaggtcc gcacgcagca cgccctctac ctcaagaacg gcgccgcggc
     3301 gcgccggtag gcacccggcg ggtcagccct ctcccctgac ccgccggagg gcccgctcca
     3361 gatcctccgg gagcggatcc tgggtccgga tccggcgtcc gctgagggga tgctcgaagg
     3421 ccagccggtg ggagtggagg aagggacggc tcaggcccag ccgctccgcg tcctggcccc
     3481 cgcccccgta ccgtcgatcg cccaggatcg gatgacccac cgaggaaaga tggacccgga
     3541 tctggtgggt ccgtccggtg cgtggcgtcg cctccaacaa ggtgacccgg gggaaacgct
     3601 cgaggacgtc gaagcgcgtc tcggccggac gcgcccccgt tgcctgcacg cggatccgag
     3661 ctccccgacg cccgagcggc gcctccacgg cgaaccgctc gtgctccacc acgccgcgga
     3721 ccagcgcgag gtaccggcgc ccgacctcgt ggcggcggaa catcgcgacc aacagggggg
     3781 cggcggcctc gctgcaggcc accaccatca ggccagaggt ccccgcatcc aggcggtgga
     3841 cgatgcccgg ccggagggga tcgccggtgt cggccagcgg cacgcccatc cccaggaggc
     3901 ggttcacgag ggtgccgctc cggcggttcg ccgtggggtg ggtcggtagc cccggtggct
     3961 tgctgaccac caccaggtcc gggtcccggt acgcgagctc taccgccggg ccttcggcgg
     4021 gcaactccac catgcccgcc aggtcgagct ccagcacctc gcctcccagc agggagaagg
     4081 acttgggacg ggatcgtccg tccacgcgca cccgacccgc ggcgatcgcg cgttggacct
     4141 cggcccgagg caggccggac agctcggccg ccaccatgtc gaggcgcccg cgcagggcca
     4201 cgtaccgggc ccccacggcc tcagcgctcc tcgcgctcgt ccctcaggcc cacgagcagc
     4261 aggaacagcg cccccaccac gatggaggcg tccgccacgt tgaacaccgg ccagacatgg
     4321 aagtcgatga agtcgatcac ccgaccggcg aggaacccgt gcccgtgcac cagccgatcg
     4381 gtgaggttgc cgagcgcgcc gccgaggatc gacccaagac cgagggcggt ggccagcctc
     4441 gacaaccggg ccgacgcgac gatgatggcg atggccacga ggaaggtggc ggtggcgaag
     4501 atccaggggg cgctctgccc cagcccgaac gccccgcccg agttcgtggt gtaggtgagc
     4561 cggagcacgt cggggatcaa cgtgatcggc gggcggccat gcagcgcgtg ctccgcccag
     4621 agcttggtcc cccggtccaa ggcgtacacc agggccgctc ccagatagag ccacatggcc
     4681 agccgacgcc cgggggccgt gggcgccccc ttacctgggg cggctctggg actgcgcgtc
     4741 cttgatgcag aggtcgacgt agggcagcgc cttcagccgg gccttctcga tgggcttccc
     4801 gcatcgcgag cacagcccgt aggtcccctc gtccatccgt cccagggccc ggtcgatctt
     4861 ggccaggagg tcccggacgt tgttctcgat ggacaggtct cgctcccgct cgaaggtgaa
     4921 cgtcccggcg tcggcgttct cgtcgtcgag cgagacctca ccagagatgt ccgattggga
     4981 gttggcgaag gtcgcttcct cgatcccctg cagctgtgcg gacagctgct ccttctcctc
     5041 cacgagacgc tcgcggaggt cggccagctc cttcttggtg tacggggact tcacggccat
     5101 ggtcgaagga cggtagcacc ctgcgatacg gggcgacaag caacgcccct caagttgccg
     5161 acaggcgcac atccagctcc tcctgggtga gtcccgacac cgcgtacacc ttggtcctcg
     5221 agcgcgttcc ggagcgcaag cggatgcgcg agggagcaat accgatggcc gaagcgaggg
     5281 ctcgcaaggc ctcggcggtg gcccgaccgg cctcgggcgc cgatcgcacc cgcaccacga
     5341 cgccgcgcga aggctcgacc tccaccgccg tcctgcccga cctgggcacc acccgcaccg
     5401 tgaccaccgc catgacgccg tgtagcatgc tcccacaggc gatgacggga acgagtagga
     5461 gacctcgcct cccgcagcga gccggggacg gtgcgagccc ggcgggacgg cacctccgaa
     5521 catcctcctc gagccgccgc ccgaacgggg cgccatccgg gtgcccccag taggcgcggc
     5581 cgggtccgcc cgtgacagcg gcaggctgtg tcggcagccg tgagcggccg ggtccgtgag
     5641 ggtccggcaa gcggggtggt accgcggggt tcacgcctcg tccctgccaa ggggacgagg
     5701 cgttcgcgtt cgtgggagga ggaacggcga tgcgacggtt cgaaccggtg gacgcgaggg
     5761 cgggcttccc cgagctcgaa cgggacatcc tggcgttctg gcgcgaggcc gacgtcttcc
     5821 accgaagcat gcagcagcgg gccggcgcgc ccgagtgggt cttctacgag ggaccgccca
     5881 ccgccaacgg caagcccggg ctgcaccaca cggagtcccg cacgttcaag gacgtgtacc
     5941 cgaggttcaa gacgatgacc ggccactccg tgccccgcaa gggcgggtgg gactgccacg
     6001 gccttccggt ggagctcgag gtggagaagg agatcgggac caagaccaag cgcgacatcg
     6061 aggccttcgg gatcggcgag ttcaaccgcc tgtgccgggc atcggtgact cgctacgtcg
     6121 acgagtggga gcggctcacg gagcggctcg gcttctggat cgacaccgcc gacgcctact
     6181 ggaccatgaa caccgagtac gtcgagagcg tctggtggtc cctgaaggaa ctgcaccgca
     6241 aggggctgct gttcgaggcc gacaaggtga ccacctactg cccccgctgc ggcaccacgc
     6301 tgtccgacca cgagacggcc ctcggctaca cgcaggtcac cgaccccagc gtgttcatcc
     6361 ggttccagat cgtggactcg ccggtgcccg agctgatcgg cgcctccctc ctgggttgga
     6421 ccaccacgcc gtggaccctg atctccaaca ccgggctcgg ggtggcggcc gatgctcgct
     6481 acgtggtggt ggagcacgag ggtgcccacc tggtcctggc cgaggcgctc cgggagcggg
     6541 tgctccccga cgcgccggtc gtcggcccgg cggtcaccgg cgcggcgctg gtggggaccc
     6601 gttaccggcc gctgtacccg aacgtggagg gggcgcaccg cgtggccgac gccgagttcg
     6661 tctccctgga ggacggcacc ggcgtggtcc acatggcacc ggccttcggg gccggagaca
     6721 tggagatcgg ccggcgcgag ggctggccgg tgttcaagcc gatcgacggc gaaggcaggt
     6781 tcacggacga ggcaccggcg ttcgtccgag ggctgttctt caaggaggcc gacgccccca
     6841 tcaccgagga gctccgcgcc cgcggtctcc tggtccgggc cgagacgatc gaccactcct
     6901 accccctctg ctggcggtgc ggcacgcccc tcctgtatta cgccaggaac tcctggtaca
     6961 tccgcaccac ggcggtcaag gagcgtctct tgcaggtcaa cgaggaggtg tcctggtacc
     7021 cggcgcacat caagcacggc cggtacggcg actggctcga gaacaacgtg gactgggccc
     7081 tctcccggga gcggtactgg gggaccccct tgcccatctg gcggtgcccg gccggacacg
     7141 acaccgcgat cggctccctc accgagctgt ccgagcgcgc cgggcgcgac ctcacggcga
     7201 tcgatccgca ccgccccgcg atcgacgagg tgaccttccc ctgcccgggg tgcggaggaa
     7261 cggccacgcg cgtgcccgag gtcatcgaca cctggtacga ctcgggcgcc atgcccttcg
     7321 cgcagtgggg gtaccacccg gagctcggcc gggggaagga gatgttcgac cagcgcttcc
     7381 cggcggactt catctccgaa gccatcgacc agacccgcgg ctggttctac acgctgatgg
     7441 ccgagggcgt cctgcacttc gactcgatct gctaccgcaa cgtggtgtgc ctgggcctga
     7501 tcgtggacaa ggacggccgg aagatgtcca agtccgtggg taacgtgatc gacccctggg
     7561 acgtgttgga ccggcagggt gccgatgccc tccgctggta cctcatcacc agcgggtccc
     7621 cctgggccgc ccggcgggtg gggatggaga tcctcgacga ggtggtccgg cggttcctgc
     7681 tcacctggtg gaacgtgtac gccttcttcg tcctgtacgc gaacgcgagc gggttcgacc
     7741 ccgccgagca cgaggtgccg ccgtcggagc ggccgctgct ggatcgctgg atcctgtccc
     7801 gactgagcca gacggtggcc gaagcccgtg agggactcga ggcgtacgac gcgacgggcg
     7861 ccggacggcg catcgcggcc ttcgtggagg acctgtcgaa ctggtacgtg cgactgtccc
     7921 gtccccggtt ctgggatccc cagggccgca tgagcgtcga cacggtcgcc gggttcctga
     7981 cgctgcacga gtgcctggtg accctctccc ggctgctggc tccgttcacg ccgttcctgg
     8041 ccgaggccct ctggcggaac ctggcggccg gccggggtga cgcgccggac tcggtccatc
     8101 tgtccgacta cccgcgcccc tcggggggca tggtggaccc gttgctcgat cgggccatgg
     8161 ccgacgcccg agaggtcgtc gagctcggac gccggatcag gaccgaggcg aaggtccgtg
     8221 tgcgccagcc cctggccgag gccgtgatcc aggttccggg cgatcaggga ccgctgcgcc
     8281 cgctgctgcc cctgatcgcg gacgagctga acgtgcgctc cgtgaccttc gccggctccg
     8341 ccgatcggtt cggatcctgg cgggccaaac cggacttccg cgccctcggc ccacggctcg
     8401 gcccgcaggt caaggaggtg gcctcggccc tgtccgagga cgacggcgcc ctggccgccc
     8461 ggctggccgg cggggagtcg gtcacggtga ggacatcgac cggcgaggtc gccctccacc
     8521 cctcggacgt ggagctggca cagaagacgg tgtccggttg gggactcgcc tccgacggct
     8581 cgatcacggt tgccctggag ctggagctca ccgaggagct ccgccgcgag ggcctcgccc
     8641 gcgagctgat ccgagcgatc caggacgcgc ggaaggccgc aggactggac gtgaccgatc
     8701 gcatcgtgct cggcgtggcg gcggatgggg ccgtagggga ggcgctccgg atgcaccggg
     8761 acgtgatcat gtccgagacc ctggccaccg tcttggagga aggctcgccc tcccacgccg
     8821 agcacgtgca ggaggtctcg gtggagggct cggccgtgac gatcgggctg cgccgagact
     8881 gaccgttcgg ccggtccccg gcggagctta ggggtggagg cctcagtcct ctccccagaa
     8941 caggtcgcgc agggatcgac cgccctcggc ctcgctcggc ggttccgtgc ccggctcgtc
     9001 cgatccctcg gcggcggcac ccaccgccgc gtccggctcc gcctcgggca cggagacggg
     9061 ctggtccggc ccggttccct cccgcgtggt ggactgctcc tgggaaccgg cgtcgggccc
     9121 ctcggctccg tgcccgtcgc gcgcagcctg gaccatctgg cggacggcct ccgcgtgctg
     9181 ctggaccagc tggccgagct cctgaaggaa cccgcgctcg cgcgccagga acggcgccat
     9241 caccgcccgg gcgtcggcgc ccgcaccgcc ccccgcggcg gctgcggcga tccgggcggc
     9301 ctcggaccgg gcctccgcta cgatccgttc ggcctcccgt cgcgctccgg ccaagatgcc
     9361 ctcggcatcc gccctggcct gacccgcggc cgcatcgttc cccaggcccg catcggcccc
     9421 tcgagccgcc tccagctcgc ctcggagccg gcgggcctcc tcttggtacg caccgacctc
     9481 ttccgtgaca tggtccagga aggcatcgac gtcccgctcg ttgtagccgc ggaaggcgag
     9541 gcggaactcc ttctgctgga cgtcctcggg cgacagccgc ggcgacagcg gatcgccccc
     9601 gaacccggtg tgttgcgagg cctcctgctt cttcttcctc atgatccccc caaggcacta
     9661 gcagcccagc gccacccgca ggacccagag gataacgaag gcgaggagga ccgagaggtc
     9721 gagccccatc ccccccatcc gcaccggcgg gatcagcgat cggaggggtc gcagcaccgg
     9781 ataggtcacg gcgtccaacg catccatcac gacccgaccg ggtccggagt agggcctgcg
     9841 gacccccagc acgaacgccc acgacaacag gacctgcgcc agcaggatga accagtagac
     9901 ggtgagcagg acgcagaccg cgctgagcac gatgttcccg gcggcggtca tcgggtcggg
     9961 accggcccct cagaactggt tgtagaagtc gccgccggcg atacgctccc ggtcctcagc
    10021 cgacacctcc atgttggcgg gggtgagcag gtacacgcgg ttggcgacca tctcgatctt
    10081 cccgtccagc ccgtagacca gaccggatgc gaagtccacc agacgccgcg cgatggtgtc
    10141 gtcggtgctc tgcaggttca tgatcacggg gatcccgccc ttgaaccggt cggccacctc
    10201 gcgggcctcg ttgaaccggc gaggctcgga cttgtggatc gacgacgcca tcgccgccct
    10261 ggtggcgggg atggtgcgca cgatgccctg atcctccggc tccacgagga cctcccggac
    10321 ggcccgcggg cgctgcaggc ggcggacggg cgccgactcg ggcgcggcga cctcgtccag
    10381 gtcctcgaac tcatcgtcct cgaccagtcc gaggtacgtc agggtcttct tccacacgcc
    10441 tggcatgagc tcctcctacg tcgtcggtgg ccgctcgccg aacagggccg ttccgatccg
    10501 gaccatggta gcgccttcct cgatcgcgac cgggaaatcc atcgacattc ccatcgagag
    10561 ctcggtgagg ctcgggtggt cccgagccag ccgatcgcgt agctcgcgca gtcggcggaa
    10621 gaacggtcga gcctcctcgg gttccgagaa gaagggcggc atcgtcatca gtcccaccag
    10681 ctggagccct tcgaccgacg ccacctcgtc gacgaaggcc gacgcccgct ccggtgccac
    10741 accggtgcga gcaccggtga agtcgacctc gatcagcgcc accagacggc ggcctccacg
    10801 gaccgctcgg cggccgaggc gccgcacggc gctcccgggg gccagggtct ggaccacgtc
    10861 ggctaggtcg gccacgtggt gcaccgtgtg gctctgcagg gtgccgatga agtgccacgt
    10921 ggcatcgggc accgctcccc gcttgtccct gagctccccc acgtagttct ccccgaactc
    10981 tcggacgccg ctctcgaagg cccagccgat cgcctcgggg cctacggtct tgctcgcccc
    11041 caccaaacgc acctcggagc gctcgcgacc ggcccgtcgg caggcctcgg cgatccgttc
    11101 gatgatccgg gcgacgttgt ccccgatcgc gtcgcgcgag cgtagcacca tgaggcgtca
    11161 gaacttaaca ggagtgaacc tcgttcacga gccttaccag ggctgggccg gcgaggatca
    11221 cttcaaccgc atccccaggg ccatgtgccg accggtcacc ccatcccgac ggtaggagaa
    11281 gaaccggcgg cggccgcagg cggtgcactc ccccgtgtcc cagatccgtc ggatcccttg
    11341 ttcgcgcagg accgcccggg tggttcccac gaggtccagg aacacccggt cgccccgacg
    11401 acgggtcatc gccccggccc ccgagcccgc cgcgacggcc agggcgacgt ccgccccgac
    11461 ctcgtagtgg cagggaccga tcgcgggccc gacggccacg cggacctcgc gccgggcgcc
    11521 gaagagcgag gccgcccgct gcatga
//
LOCUS       DASQQQ010000041        13047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_141084, whole genome shotgun sequence.
ACCESSION   DASQQQ010000041 DASQQQ010000000
VERSION     DASQQQ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13047
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141084"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VE646_02035"
     CDS             <1..322
                     /locus_tag="VE646_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78806.1"
                     /translation="QPLKPTPLRSQTLSGLHQTVHSGELTPDVALEHLERIWRMRRRL
                     LGDAVLRRRAWDVAESMGWADPYTAEHIALTQLQADAFITVDTELAHSVEGLVHTASI
                     DAPR"
     gene            525..1022
                     /locus_tag="VE646_02040"
     CDS             525..1022
                     /locus_tag="VE646_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78807.1"
                     /translation="MDKLDQIRRLRDEYEAAMDETERLRDKYHQAIVTLHRSGATLRE
                     IAVGLGISHQRVHQIVSPTTTRSRSRARVASVGAALIVALVLGGFAIRTATAPDSDRA
                     SSTVEHSAETEPPVPSNSAVVTLNPRTGQILAGTVVILDPNTGKILALRASPLADLLE
                     NTPPK"
     gene            1043..1912
                     /locus_tag="VE646_02045"
     CDS             1043..1912
                     /locus_tag="VE646_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HZD78808.1"
                     /translation="MSSLMYRSFFALGDGIRTPDGRPIGAVHGYLDMVRRLIVARRPG
                     QVVHVYDHEWRPMARTDLYPGYKSKRPPDPDGLPEQFEVLHRVLDLTGMPQAQTIGWE
                     AEDAVGALCAGAEETDRIGIVSGDRDLLQLVRDPGVRLLYTLRGVTEMREFDEAMVRE
                     VYGVPAHRYVDFAILRGDPSDDLPGVRGVGEKTARALVLTYPTLDELLADASATEPRP
                     GPLKGKPALRARLRDAADYIATMRRLVPVNADAPLDLWSGGRDDAALSALADGLGLRG
                     PIQRLRAALDSAG"
     gene            complement(1918..5151)
                     /locus_tag="VE646_02050"
     CDS             complement(1918..5151)
                     /locus_tag="VE646_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZD78809.1"
                     /translation="MEKFIGDAVMAVWGTPVATEDDAERAVRAALDLTKAVTVLGEEL
                     GELELLARAGVLTGEAAVNLAAQGEGMVAGDLVNTASRIQSAATPGQVFVGEATRRAT
                     EASFAYEDAGTFELKGKAGLVPLWRAVRVVGGVRGTLRSVGLEPPFVGRDRELRMVKE
                     LFHASADERRAHLVSVLGIAGIGKSRLAWEFYKYFDGLADDTFWHRGRCLSYGEGVTY
                     WALAEMVKMRARIAEEEEPASALEKLRATVEENVPEPEERRWIEPRLAHLLGLEERTA
                     ADKEDLFAAWRLFFERLAERFPVIMVFEDMQWADSSLLDFIEYLLEWSRSSPIFVMTL
                     ARPEFTERRPTWGSGKRNFTSLYLEPLSHGATHELLSGLVTGLPHETEQRILERAEGI
                     PLYAVETVRMLLDRGLLVAEGTGYRPTGPIETLEVPETLHALIAARLDGLTGSERRAV
                     QDAAVLGKTFFAVGLASVTDQREVELEPVLASLVRKEILSVQADPRSPERGQYGFLQD
                     LLRTVAYETLSKRERKTKHLAAAAFMERLWVGDVGEIAEVLASHYVQAYRSAPEAEDA
                     AQIRSRARELLSRAGEHAASLAAAEEALRYFEQAIGLTEQPLGQAELHELAGRMAWQA
                     RQAPTAMEHYEAAIGIFDSIGLTHPSARVSAALAEAIWQEGRIEEAVERMRSAFGVLS
                     GEEEDADLATLAAQLGRLLYFMGRTDEALERIELALSIAEALRLPEVFSQALNTRGLI
                     LSTHGRFEEGSLLLRHSLKVAVDNDLSGAALRAYNNVAAQAGQEDAFEDVIEFSREGL
                     ELARRVGDRGWELSFLIGDLGELVYLGRWDEAEARVAAQPPSMDMTEAIRMSYLGMIP
                     MYVWRGDLDRARELLALFPDGESAGDVQTRTVFRALQAPLLRAEGRLEEALAAGEEAF
                     AVHAEFGIRAPQVKEGLVEALEAAGAMGDVDKAEELLRRIEAMRPGEISPYLQAQGAR
                     FAGRVAALRRDDERVEPGFQAASERFAKLKLPFHLAVAKLEQAGWLLDHSAGERAELL
                     LAEARTTFEDLGARPWLERCDRALARPSGGLDELEPSAAAGDGHGPS"
     gene            complement(5124..5531)
                     /locus_tag="VE646_02055"
     CDS             complement(5124..5531)
                     /locus_tag="VE646_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HZD78810.1"
                     /translation="MPAHQGEAVRCASCGTENEPGRKFCGECGAPLSGACPACGTPNP
                     PTVKFCGECGSSLAAEPVPVPPSVPTTPLPEGPVAERRLVTVLFADLVGFTALSERRD
                     AEEVRELLSRYFDTCRTLIARYGGSWRSSSAMP"
     gene            complement(5600..6520)
                     /locus_tag="VE646_02060"
     CDS             complement(5600..6520)
                     /locus_tag="VE646_02060"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010888990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HZD78811.1"
                     /translation="MKVGVVGAGFVGAAAANALVLRHVATDVVLVDVNETKAAAEAAD
                     IAHVTPFSAPVRVGAGGYEQLAGSAVVVITAGRAQQPGQSRLELLRANARILRSVVAE
                     VVAAAPDAVLLVATNPVDVMTRVIEQHAGLGGGRVFGTGTMLDTARFRQIVAARVGVD
                     VTHVHGYVVGEHGDSEVLIWSSLDVAGRHLAAFVHAMGGAFGQEDRAEVEDEVVNAAE
                     RIIRGKGATYYGVAAAIAGAVEVILRNRRSIMTVSAWTPEYGCSLSLPRLVSGPGVIR
                     ELGVSMDGGERSRLERSAEILRGALAQIGT"
     gene            complement(6562..8619)
                     /locus_tag="VE646_02065"
     CDS             complement(6562..8619)
                     /locus_tag="VE646_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HZD78812.1"
                     /translation="MARLPVRGAPRLVVLSFLMLFTELALIRFTSAYIVYLKYFTNFV
                     LLASFLGIGVGFLRAGARHDRSALAPFALLAVVVFVMIFPVRQTPGQSGPILRGLFGT
                     PPLPVWVSLPLLFLAVTAAMACIGEGVARVFVRFDALQAYRLDILGSLLGIAAFTLGS
                     FLHAGPIVWGLVIAAALAVTSEWPIGTVRVLALGAFVLLLVGTSVLSGDLWSPYYRIG
                     VGRDAGSRIPITVNGLPHQSILPFRELVRRQPFYLYPYEHLRSRPRRVLIVGAGSGND
                     VAVALREGAGSVDAVEIDPLLQRLGRARHPDHPYADPRVRAIVDDGRAFLERTDARYD
                     LIEFALPDSLTLVSGAGSLRLESYLFTREAFQTVRDHLAAGGVFSMYNYYRPDVFDRY
                     AGTLSEVFGHAPCLDRGLPGAGPRRQAVLTIGVHPSSIVCTTRWQPTGPVPAPATDDY
                     PFPYARSRGLPAFYLVSLLFVLLASAVLVRTIGGEPLRGIGRYADLFAMGAAFMLLET
                     KNVVQFALLFGTTWLVNSLVFAGILLAVYLAVEVSRRVRFRLLWPSYAALVAALAVAW
                     SVPQERLLSLAPVPRFVAAATVAFAPVFLANLVFAQRFRDSGSPTLAFGANLLGAMVG
                     GVLEYGALIVGYRDLLIAVALLYGVAFWLQARGRAVSAGEREMVGPGEPVLEPSTAPP
                     SNA"
     gene            complement(8682..9161)
                     /locus_tag="VE646_02070"
     CDS             complement(8682..9161)
                     /locus_tag="VE646_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HZD78813.1"
                     /translation="MGEDTAAIRAAREAGLAFDVVRTSPPSSVEESAALQGIDAGSLL
                     RTIVVRRGADDYVFVLVPGGRQIDWPRLRAHLRVNRLSLPDKEEAREVTGYERGAITP
                     FGSSRAWPVVADVSISGLGRVAIGGGARGVNLHLASTELTRVLAATLAAVTRPAERA"
     gene            9234..10520
                     /locus_tag="VE646_02075"
     CDS             9234..10520
                     /locus_tag="VE646_02075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005443987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZD78814.1"
                     /translation="MAVGRSSSEVARTVERLIGPDLAIAVETYDGGRLGPVDAPATLV
                     LRSPDALRRILTAPGELGVGRAYVAGDLDVRGDVYAALIALRERLPEVRMGPTQLAAL
                     VRLVGTRDLRPLPAPPEEARLRGRRHSRRRDAAAIAHHYDVSNRFYELVLGPSMTYSC
                     AVWEGPDLTLENAQAAKYELICRKLALRPGVRLLDVGCGWGGMAVHAAEHHGARVVGV
                     TLSRRQVEWAQKAVAGAGLADRVEIRFQDYRDVRDGPFDAVSSIGMFEHVGLRNLSLY
                     FRHLHRLLRPEGRLLNHGISRPARVGGHTRRGVPVTPSRLQRRSFVDRYVFPDGELHE
                     VGAAVSTMQAAGFEVRHLETLREHYGLTLRAWVSNLEANWEEAAREAGEARARIWRLY
                     MAASALNFQAGRTQVHQVLAVKAPKGRSGFPLRPPF"
     gene            10578..10964
                     /locus_tag="VE646_02080"
     CDS             10578..10964
                     /locus_tag="VE646_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZD78815.1"
                     /translation="MEIARGNEGTYQAGDDARFTGAAWLRSTLAAEDGTNVGVVTFAP
                     GARTHWHRHPGGQFLFGLSGRGRVRTRGERGHVLEAGDVVRVGPDEWHFHGGTPDTLM
                     VHVAVNGGGAPEWGDPVTDEEYAEGF"
     gene            complement(11037..11939)
                     /locus_tag="VE646_02085"
     CDS             complement(11037..11939)
                     /locus_tag="VE646_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZD78816.1"
                     /translation="MARPLRVGVQLPEVERVVRWPEYLSMARAAEEVGFDSVWVGDHL
                     LYRGGDGRSERGPWEAWTLLSALAAATERVRLGPLVACLAFHPPAVLAKAAATVAEVS
                     VGRFVLGVGSGWNEAEFLAFGIPFDHRVDRFEEAFHVVRGLLAGERVSLDGRYHEVRD
                     AVLLPSPVHRPSLVLGSSGPRMLSIALPHVEAWNTWFDLYGNTPEGFAVETRKVDRAA
                     ERAGLDPARIQRSACVLVVLDRSSRERPDEAGVRPLEGPMERIAAGLRELAEAGAHEA
                     ILVVDPITERSIRSLGEALELLET"
     gene            complement(12003..12479)
                     /locus_tag="VE646_02090"
     CDS             complement(12003..12479)
                     /locus_tag="VE646_02090"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HZD78817.1"
                     /translation="MPEDEDEKERLNRKLTELLNELRVALPGVQVLFAFLLILPFNSG
                     FERASDLERAVYAVSLCSAAVASALFIAATAYHRHRFARLEKETLRDKTEMIAAQDHF
                     AVGGLFFLSIAMGGSVFVVFDVLFGVATAAGLALALVVVFGWFWYALPLSRRIRRG"
     gene            complement(12492..>13047)
                     /locus_tag="VE646_02095"
     CDS             complement(12492..>13047)
                     /locus_tag="VE646_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZD78818.1"
                     /translation="WKIVIENYNECLHCPSIHPELVQVVPMFRFGEVWDEETRDDGNP
                     MVEGATSFTASGRSELPKFPDLAPEDYRMYYGSYEFPNLMLNLHPDCVMYYIAFPKGP
                     SHTTVVSEYLFRPDVIDGDDFKPEPVVDFWDLISRQDWEVCARVQIGVGSRAYTRGVY
                     PRQDRFLYWFNEEYRLAMGRQLRG"
BASE COUNT         2035 a         4842 c         4429 g         1741 t
ORIGIN      
        1 ccagccgcta aagcccacac cgttgcgatc ccagacgctg tcgggcctgc accagaccgt
       61 ccacagcggc gagctcaccc ccgacgtggc gctcgagcac ctggaacgga tctggcggat
      121 gcggagacgg ctgctcggcg acgccgtcct ccggcgtcgg gcctgggacg tggccgagag
      181 catgggatgg gcggatccct acaccgccga gcacatcgcc ctgacccagc tgcaggcgga
      241 cgcgttcatc accgtggaca cggagctggc acacagcgtg gaggggctgg tccacaccgc
      301 gtcgatcgac gccccgcgat aaaggagagg cggcatcgaa ggcgacgcta ccccccggtc
      361 cctcggcctc gtccctctga tcgacgacct gcgggtgggg agcgaggagg cggagctcgg
      421 tgcataggct gctgctggac gtgtcgaggt gtcgttcagg taccgaaccc tctgtctacg
      481 cccccttgac gccggtggcg tcgcttcgct agtgtctaga gggtatggac aagctcgacc
      541 agatccggcg gcttcgcgac gaatatgaag ccgcgatgga cgaaactgag cggcttcgcg
      601 acaagtacca ccaggccata gtcactctgc accgctcagg cgcgactctc cgtgagatcg
      661 cggtcgggct gggaatctcg caccaacgcg tgcaccagat cgtgtcacca accaccacgc
      721 gttcacgatc gcgagcgaga gtggcctctg tcggcgctgc cctcatcgtc gctctcgttc
      781 ttggtggatt cgcgatccgc accgcgacgg cgcccgacag tgaccgagcg tcctccaccg
      841 tcgagcattc ggccgagact gagccacccg tcccttccaa cagcgccgtc gtgacgctga
      901 acccgcggac cgggcagatc ctggccggga cggtggtgat tctggatcca aacaccggca
      961 agatccttgc cctcagagcc tcccccctcg ctgacttgct cgagaacacg ccgccgaagt
     1021 gacctcgagg ctgcttcttg acgtgtcgag cttgatgtac cggtcgttct tcgcgctggg
     1081 ggacgggatc cggacccccg acgggcggcc gatcggcgcg gtccacggct acctggacat
     1141 ggtccggcgc ctgatcgtcg ctcgtcgtcc cggacaggtg gtgcacgtct acgaccacga
     1201 gtggcggccc atggcccgca ccgacctgta ccccggctac aagtccaagc gtcctcccga
     1261 ccccgacggc ctccccgagc agttcgaggt gctccatcgg gtgctggacc tcaccggcat
     1321 gccgcaggct cagaccatcg gatgggaggc cgaggacgcc gtcggcgccc tgtgcgccgg
     1381 agctgaggag acagacagga tcgggatcgt gagcggcgac cgagacctcc tccagctggt
     1441 gcgcgacccg ggcgtgaggc tcctctacac gctccggggg gtgaccgaga tgcgggagtt
     1501 cgacgaggcc atggtccgcg aggtgtacgg cgtgccggcg caccgctacg tcgacttcgc
     1561 catcctccgc ggcgacccca gcgacgacct gccgggcgtc cggggggtcg gagagaagac
     1621 ggcccgagcg ctggtgctca cctacccgac gctggacgag ctcctggccg acgcgtccgc
     1681 caccgagccc cggccggggc ccctcaaggg caagcccgcc ctgcgagcgc ggctccggga
     1741 cgccgccgac tacatcgcca cgatgcgccg gctcgtcccg gtcaacgccg acgcaccgct
     1801 ggacctctgg agcggcggcc gggacgacgc ggcgttgagc gcgctggccg atggcctcgg
     1861 gctgcggggg ccgatccaac gcctgcgggc cgccctcgac tcggcgggct gaaccggcta
     1921 cgagggaccg tggccgtcac ccgccgcggc gcttggctcc agctcgtcca gcccgcccga
     1981 cggccgggcc agcgcacgat cgcaccgctc gagccagggg cgagccccca ggtcctcgaa
     2041 cgtcgtgcgc gcctcggcca ggagcagctc cgcccgctcc cccgccgagt gatccagcag
     2101 ccatccggcc tgttccagct tggccacggc caggtggaac ggcagcttca gcttcgcgaa
     2161 ccgctcggac gcggcctgga atcccggctc cacgcgttca tcgtctcggc ggagggcggc
     2221 cacacgaccg gcgaacctgg cgccctgcgc ctggaggtag gggctgatct cccccggccg
     2281 catcgcctcg atccgccgca gcaactcctc ggccttgtcc acgtcgccca tcgcgccggc
     2341 cgcctcgagg gcctccacca gcccctcctt cacctgcggg gcacggatcc cgaactcggc
     2401 gtggacggcg aaggcttcct cgcccgcggc cagcgcctcc tccagccgtc cctccgcccg
     2461 cagcaggggt gcctgcaagg cccggaacac cgtgcgggtc tgcacgtcgc ccgcggactc
     2521 cccgtcgggg aacagcgcga gcagttcacg cgccctgtcc aggtcgcctc gccagacgta
     2581 catcgggatc atgcccaggt acgacatgcg gatcgcctcc gtcatgtcca tgctgggggg
     2641 ctgggccgcg acccgggcct cggcctcgtc ccaccgcccc aggtacacga gctcgcccag
     2701 atcgccgatc aagaagctca gctcccaccc gcgatcgccc acccggcgag cgagctccag
     2761 gccttcccgg ctgaactcga tcacgtcctc gaaggcgtcc tcctgaccgg cctgcgccgc
     2821 gacgttgttg taggcacgca gggccgcccc ggacagatcg ttgtccacgg ccaccttcag
     2881 ggagtggcgg agcaggagcg atccctcctc gaaccgaccg tgggtggaca ggatcagccc
     2941 ccgcgtgttg agggcctggg agaacacctc cggtagacgg agcgcctccg cgatggacag
     3001 cgccaactcg atccgctcca gcgcctcatc cgtcctgccc atgaagtaca ggagccggcc
     3061 gagctgcgcc gccagggtgg ccaggtcggc atcctcctct tcacccgaga ggaccccgaa
     3121 ggccgagcgc atccgctcga cggcttcctc gatgcgtccc tcctgccaga tcgcctcggc
     3181 cagcgccgct gagacccggg ccgacgggtg ggtgagcccg atcgagtcga agatcccgat
     3241 cgccgcctcg tagtgctcca ttgcggtggg ggcctgtctg gcctgccagg ccatacgccc
     3301 ggcgagctcg tgcagctccg cctgcccgag gggctgctcg gtcagcccga tggcctgctc
     3361 gaagtagcgg agcgcctcct ccgcggcggc caacgacgcc gcgtgctcgc ccgccctgct
     3421 caacagctcg cgggcgcggg acctgatctg cgccgcatcc tccgcctccg gcgccgagcg
     3481 gtaagcctgc acgtagtgag aggcgagcac ctcggcgatc tcccccacgt cgcccaccca
     3541 caagcgttcc atgaacgcgg ccgcggccag gtgcttggtc ttgcgctcgc gcttggacaa
     3601 ggtctcgtac gcgaccgtgc gcagcaggtc ctggaggaac ccgtactggc cccgctccgg
     3661 tgagcgggga tcggcctgga ccgacaggat ctccttgcgg accagcgagg cgagcacggg
     3721 ctccagctcc acttcccgct gatcggtcac cgatgccagc ccgacggcga agaaggtctt
     3781 gcccagcacg gcggcgtcct gcacggcgcg gcgttcgctg cccgtgagcc cgtccaggcg
     3841 ggccgcgatg agcgcgtgca gcgtctcggg gacctcgagc gtctcgatcg gcccggtggg
     3901 tcgatagccg gtgccctcgg caaccaggag cccccggtcc aggagcatcc gcacggtctc
     3961 cacggcgtac agagggattc cctcggcccg ctcgaggatc cgctgctcgg tctcgtgggg
     4021 gaggccggtc accaatcccg acaggagctc gtgcgtggcc ccgtgggaca gcggctccag
     4081 gtagagcgag gtgaagttgc gcttgcccga tccccaggtg gggcggcgtt ccgtgaactc
     4141 gggacgggcc agggtcatga cgaagatggg ggagctccgc gaccactcca acaggtactc
     4201 gatgaagtcc agcagcgagc tgtcggccca ctgcatgtcc tcgaagacca tgatcacggg
     4261 gaaccgctcg gccagccgct cgaagaacag ccgccaggcg gcgaacaggt cctccttgtc
     4321 cgcggccgtc cgctcttcca gccccagcag gtgcgccagc cgtggttcga tccagcggcg
     4381 ctcctccggc tcgggcacgt tctcctccac ggtggcccgg agcttctcca gggccgaggc
     4441 cggctcctcc tcctcggcga tgcgggcccg catcttcacc atctcggcga gcgcccagta
     4501 ggtgaccccc tccccgtacg agaggcaccg cccccggtgc cagaaggtgt cgtcggccag
     4561 cccgtcgaag tacttgtaga actcccaggc cagccgggac ttgccaatgc ccgcgatccc
     4621 cagcacggag accaggtgcg cacgacgttc gtcggccgac gcgtggaaca gctccttgac
     4681 catccgcagc tcgcggtcgc gtcccacgaa gggcggctcc agtcccacgg acctcagcgt
     4741 gccccgcacg ccgcccacca cccggaccgc gcgccacagg gggaccagcc ccgccttgcc
     4801 cttgagctcg aacgtaccgg cgtcctcata ggcgaacgaa gcctctgtgg cccgccgggt
     4861 ggcctcgccc acgaagacct gccccggcgt ggccgccgat tggatccggc tggcggtgtt
     4921 gaccaggtcc ccggccacca tgccctcgcc ctgggccgcc aggttgaccg ccgcctcgcc
     4981 ggtgagcacg ccggctcgcg ccagcagctc gagctccccc agctcctccc ctaggacggt
     5041 caccgccttc gtgaggtcca gggcagcgcg gaccgcccgt tcggcatcgt cctccgtggc
     5101 cacgggcgtc ccccacaccg ccatcacggc atcgccgatg aacttctcca cgatcccccg
     5161 taccgcgcga tcagggtccg gcaggtgtcg aagtaccggg acaggagctc acggacctcc
     5221 tcggcgtccc ggcgctccga gagcgccgtg aaccccacca gatcggcgaa caggacggtg
     5281 accagtcggc gttcggccac cggcccctcc ggtaggggag tggtcgggac gctcggcggg
     5341 acggggacgg gctcggccgc gagcgatgac ccgcactcgc cgcagaactt cacggtcggg
     5401 gggttcggcg tgccgcacgc gggacaggct ccggataggg gggcgccgca ctcgccgcag
     5461 aatttccgac cgggctcgtt ctccgtcccg cacgacgcgc accgcacggc ttccccctgg
     5521 tgagcgggca tgacggccta accgtacacc gaacgggtcc ggcccgggcc gggcgtggga
     5581 cccgtcgcgg ccctccgcct cacgtgccga tctgggccag cgccccgcgc aggatctccg
     5641 ccgaacgctc cagtcgcgag cgctcgccgc cgtccatcga cacgccgagc tccctgatca
     5701 cgccgggccc gctgaccagc ctcggcagcg acagggagca cccgtactcg ggtgtccagg
     5761 cgctcacagt catgatcgat cggcggttcc gcaggatcac ttccacggcc ccggcgatgg
     5821 ccgcggccac cccgtaatag gtggcaccct tgccccggat gatgcgctcg gccgcgttca
     5881 ccacctcgtc ctccacctcg gcgcggtcct cctgaccgaa cgcgcccccc atcgcgtgga
     5941 cgaaggccgc caggtgacgc ccggccacgt ccaggctgct ccagatcagc acctcggagt
     6001 ccccgtgctc cccgaccacg tagccgtgga cgtgcgtcac gtccacgccc acccgcgcgg
     6061 cgacgatctg ccggaaccgc gccgtgtcga gcatggtgcc cgtcccgaag acccgtccgc
     6121 cgccgagacc ggcgtgctgc tcgatcacgc gggtcatcac gtcgaccggg ttggtggcca
     6181 cgaggagcac ggcatcgggg gcggcggcca cgacctcggc caccaccgag cgcaggatcc
     6241 gagcgttcgc tcggagcagc tccagtcgag actggcccgg ctgctgggcc cgccccgccg
     6301 tgatcaccac gacggccgag cccgccagct gctcgtaacc gccggccccc acccggaccg
     6361 gcgccgagaa gggcgtcacg tgggcgatgt ccgcggcctc ggccgccgcc ttggtctcgt
     6421 tcacgtcgac caggaccaca tcggtcgcca cgtggcggag caccagcgcg ttcgccgccg
     6481 cggcgcccac gaacccggcc cccaccacgc ccaccttcat cccggcaccc taccgcggtg
     6541 ggccgccccg ccggcctccg gtcaggcgtt cgatggtggc gccgtggagg gctcaaggac
     6601 gggctctccg gggcccacca tctcgcgctc ccccgccgag accgctcgac cgcgagcctg
     6661 cagccagaac gccacgccgt agagcagggc cacggcgatc aggaggtcgc ggtagcccac
     6721 gatcagcgct ccatactcca acaccccgcc gaccatcgct cccagcaggt tggcgccgaa
     6781 ggccaaggtc ggggatccgg aatcgcggaa ccgctgcgcg aacacgaggt tcgccaggaa
     6841 caccggtgcg aacgccaccg tcgccgccgc gacgaaccgg ggcacgggag ccagagacag
     6901 aagccgttcc tggggaacgc tccacgccac cgccagagcg gccaccaacg cagcgtagga
     6961 gggccagagc agccggaacc gcacccgccg ggagacctcg accgccaggt agacggcgag
     7021 caggatcccc gcgaagacga gcgaattcac cagccaggtg gtcccgaaca gcagggcgaa
     7081 ctgcacgacg ttcttggtct ccaggagcat gaacgcggcg cccatcgcga acaggtccgc
     7141 gtaccggccg atcccgcgca acggctcacc gccgatcgtg cgcaccagca ccgccgaggc
     7201 caggagcacg aacagcagcg agaccaggta gaacgccggc aggccgcggc tgcgcgcgta
     7261 ggggaagggg tagtcatcgg tggcaggcgc gggcaccggc ccggtgggct gccaccgggt
     7321 ggtgcagacg atcgagctcg gatgcacccc gatcgtcagg accgcttgtc gcctcggtcc
     7381 cgcgccgggg agccctcggt ccaggcacgg ggcgtgtccg aagacctcgc tcagggtgcc
     7441 cgcgtaccgg tcgaagacgt cgggccggta atagttgtac atgctgaaga cgccaccggc
     7501 ggccaggtgg tcccggaccg tttggaaggc ctcgcgtgtg aacagatagc tctccagccg
     7561 gagcgacccc gcacccgaca cgagggtcag ggagtcgggc aaggcgaact cgatcaggtc
     7621 gtaccgggcg tcggtccgct ccaggaaggc gcgtccatcg tccacgatgg cccgaacccg
     7681 aggatcggcg tacggatggt ccggatggcg cgcccggccc aagcgctgca gcagcgggtc
     7741 gatctcgacc gcgtccacgg agccagcgcc ctcgcgcagg gccaccgcca cgtcgttgcc
     7801 ggagccggcc ccgacgatga gcacccgccg gggccggctc cggagatgct cgtacgggta
     7861 caggtagaaa ggctgccgtc tgaccagttc gcggaagggc aggatcgact ggtgcgggag
     7921 cccgttcacc gtgatgggga tccggctccc ggcgtcccgg cccaccccga tccggtagta
     7981 gggggaccac aggtccccgg agagcacgga ggtcccgacg agcagcagca cgaacgctcc
     8041 cagggccagg acgcggaccg tgccgatcgg ccactcggac gtgaccgcca gcgcggcggc
     8101 gatcaccagc ccccagacga tcggcccggc gtggaggaac gatccgagcg tgaacgcggc
     8161 gatccccagc aggctgccca ggatgtccag gcggtacgcc tgcagtgcgt cgaagcgcac
     8221 gaacaccctg gcaaccccct cgccgatgca ggccatggcc gcggtgaccg ccaggaagag
     8281 caggggcagc gagacccaga ccggaagtgg cggggtaccg aacagacccc gcaggatggg
     8341 gcccgactgt ccgggcgtct gccgcacggg gaagatcatc acgaagacca ccaccgcgag
     8401 cagcgcgaac ggggctagag cggatcggtc gtgccgtgcc ccggcgcgca ggaaccccac
     8461 cccgatcccc aggaagctcg ccaggagcac gaagttcgtg aagtacttca ggtagacgat
     8521 gtacgcgctc gtgaacctga tcagggcgag ctcggtgaac agcatcagga agctgagcac
     8581 gacgagcctc ggggcgcccc tcaccggcag ccgagccatg gcgtcctcct ccccccgccg
     8641 cccggggacg aaacgctcgc ggcggtccgg cgcaacgacg ctcaggcccg ctcggcgggg
     8701 cgggtcaccg cggccagcgt ggccgccagg acgcgggtga gctcggtgga ggccaggtgc
     8761 aggttcaccc ctcgggcacc gcccccgatc gccacgcgac ccagcccgga gatggatacg
     8821 tcggcgacca ccggccaggc ccgcgacgaa ccgaaggggg tgatggcccc gcgctcgtag
     8881 ccagtcacct cccgggcctc ctccttgtcc gggagcgaga gccggttcac ccgcagatgc
     8941 gcccgcagcc tgggccagtc gatctgacgg cctcccggca ccaacacgaa cacgtagtcg
     9001 tccgctcccc gccgcaccac gatcgtgcgc agcaggctcc cggcgtcgat gccctgcagg
     9061 gcggcgctct cctccacgct ggacggcggg ctcgtcctca ccacgtcgaa ggccaggccc
     9121 gcctcacggg cggcacggat ggcggccgtg tcctcgccca tggcccggca gcctacccgg
     9181 tgcgctcggc caggtggcgc gggcgaccgg cccgggcgga tgatggcccc aggatggcgg
     9241 tgggtcgatc gtcctcagag gtggcgcgga cggtggagcg gttgatcggg ccggacctcg
     9301 ccatcgccgt agagacgtac gacggcggac ggctgggacc ggtcgacgcg ccggccacgc
     9361 tggtgctgcg ctcacccgac gcgctccggc gtatcctcac ggccccgggc gagctcggcg
     9421 tgggacgcgc gtacgtggcg ggggacctcg acgtgcgcgg ggacgtctac gcggccctga
     9481 tcgccctgcg ggagcgcctg cccgaggtcc ggatgggtcc cacgcagctg gccgcgctgg
     9541 tccgactggt cggcacccgc gacctccgcc cgctccctgc accaccggag gaagccaggc
     9601 tccgaggccg gcgccactcc cgacggcgag acgcggcggc gatcgctcac cactacgacg
     9661 tgtccaaccg cttctacgag ctcgtgctgg ggccatccat gacgtactcg tgtgcggtgt
     9721 gggaggggcc ggacctcacc ctggagaacg cccaggctgc gaagtacgag ctgatctgcc
     9781 gaaagctcgc cctgcgcccg ggggtgcggc tgctcgacgt ggggtgcggc tggggcggga
     9841 tggcggtgca cgccgccgag catcacggcg cccgagtggt gggcgtgaca ctgtcccgcc
     9901 gccaggtcga gtgggctcag aaggcggtcg ccggtgccgg tctggccgat cgggtggaga
     9961 tccgcttcca ggactaccgg gacgtccggg acggtccgtt cgacgcggtc tcttcgatcg
    10021 gcatgttcga gcacgtaggc ttgaggaacc tgtccctgta cttccgccac ctccaccgcc
    10081 tgctccgacc ggaggggcgg ctgttgaacc acggcatcag ccggcccgcc cgggtgggag
    10141 ggcacacccg gcggggcgtc cccgtcacgc cgtcacgcct ccagcgccgg agcttcgtgg
    10201 accggtacgt gttccccgac ggcgagttgc acgaggtcgg ggccgcggtg tccacgatgc
    10261 aggcagccgg tttcgaggtt cggcatctcg agaccctccg cgagcactat ggactgaccc
    10321 ttcgggcgtg ggtctcgaac ctcgaggcga actgggagga ggctgcgcgc gaggcggggg
    10381 aggcgcgggc ccggatctgg cgcctgtaca tggcggcgag cgcgctgaac ttccaagccg
    10441 gtcgcaccca ggtgcaccag gtgctcgccg tgaaggcgcc caagggccgc agcggcttcc
    10501 ctctccgccc gcccttctga gcctcggtgg gaccccatcg ccggtaggct cgacccatcc
    10561 gacgggaagg agccatcgtg gagatcgctc gggggaacga gggcacgtac caggcggggg
    10621 acgacgcacg cttcaccgga gcagcgtggc tgcggagcac cctggccgct gaggacggca
    10681 ccaacgtcgg ggtggtgacc ttcgcaccag gggcgaggac gcattggcac cggcacccgg
    10741 gggggcagtt cctgttcggg ctgtccggcc gggggcgcgt gcgaacccga ggcgagcgtg
    10801 gccacgtgct cgaggccgga gacgtggtcc gggtcggccc cgacgagtgg cacttccatg
    10861 gcggcacacc ggacacgttg atggttcacg tcgccgtgaa cggagggggc gcccccgagt
    10921 ggggtgatcc ggtgaccgac gaggaatacg cggaggggtt ctgagggagc cggacccgac
    10981 ccgttgaggc ttccgaggcc cctgcgtccg gctcgccgtc tcagaccgtg aaccgctcag
    11041 gtctcgagga gctcgagcgc ctcaccgagg ctccggatcg accgctccgt gatggggtcc
    11101 accaccagga tggcctcgtg ggctcccgct tccgccagct cccggagccc cgccgcgatc
    11161 cgctccatcg ggccctccag cgggcggacc cccgcctcat ccgggcgctc gcgcgaagag
    11221 cggtcgagca ccaccaggac gcaggcgctg cgctggatcc gagccggatc gagcccggcg
    11281 cgctcggcgg ccctgtccac cttcctcgtc tccacggcga agccctcggg cgtgttgccg
    11341 tacaggtcga accaggtgtt ccacgcctcg acgtggggca gcgcgatcga cagcatccgg
    11401 gggccgctgc tgccgagcac caagctcggg cggtgcacgg ggctcgggag cagcacggcg
    11461 tcccgaacct cgtggtagcg accgtccagc gatacccgct cccccgccag caggccacgg
    11521 accacgtgga aggcctcctc gaagcggtcc acccggtggt cgaaggggat cccgaaggcc
    11581 aggaactcgg cctcgttcca gcccgagccg acgccgagca cgaagcgccc gacgctcacc
    11641 tcggccaccg tggcggccgc cttggccagg acggccgggg gatggaaggc caggcaggcc
    11701 accagcggac ccagccggac ccgctccgtc gccgcggcca gggccgagag gagggtccac
    11761 gcctcccacg gaccacgctc ggaccgtccg tcaccacccc ggtacaggag gtggtcgccc
    11821 acccagaccg agtcgaaccc cacctcctcg gcggcccgcg ccatggagag gtactcgggc
    11881 cagcggacca cccgctccac ctcgggcagc tggacgccga cgcgcagggg tctggccacg
    11941 cggcgaagtg tatccgtccg ccgccaccgg ccgtccccca ggggacgtcc gatcaccgcc
    12001 cctcatccgc gccggatccg ccgggacagc gggagcgcgt accagaacca gccgaacacg
    12061 acgaccaagg ccagcgcgag cccggcggcc gtggccacgc cgaacaacac gtcgaacacc
    12121 acgaagacgg atccgcccat cgcgatcgac aggaagaaca gcccccccac cgcgaagtga
    12181 tcctgcgccg cgatcatctc ggtcttgtcc cggagcgtct ccttctccag ccgcgcgaac
    12241 cggtgtcggt ggtacgccgt cgcggcgatg aagagggccg acgccaccgc cgccgagcac
    12301 agcgagactg cgtagaccgc acgttcgagg tcgctcgcgc gctcgaaccc cgagttgaac
    12361 ggcaggatca gcaggaaggc gaagagcacc tgcacacccg gcagggccac ccggagctcg
    12421 ttcaggagct cggtgagctt gcggttcagc cgctccttct cgtcctcgtc ctccggcatc
    12481 ctgccggcgt cttaccccct cagctggcgc cccatggcca gccggtactc ctcgttgaac
    12541 cagtacagga accggtcttg cctcgggtac accccccggg tgtacgcgcg ggagcccacc
    12601 ccgatctgga ctcgggcgca gacctcccag tcctgcctgc tgatgaggtc ccagaagtcc
    12661 accaccggct cgggcttgaa gtcgtccccg tcgatcacgt cgggccggaa gaggtactcg
    12721 gagacgaccg tggtgtggga cggccccttc gggaacgcga tgtagtacat cacgcagtcc
    12781 gggtgcaggt tcagcatcag gttcggaaac tcgtagctgc cgtagtacat cctgtagtcc
    12841 tcgggcgcga ggtccgggaa cttgggcagc tccgaccgcc cggacgcggt gaagcttgtg
    12901 gcaccctcca ccatggggtt gccgtcgtcg cgggtctcct cgtcccacac ctcgccgaag
    12961 cggaacatcg ggaccacctg gacgagctcg gggtggatgc tcggacagtg gaggcattcg
    13021 ttgtagttct cgatcacgat cttccag
//
LOCUS       DASQQQ010000042         2414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_388470, whole genome shotgun sequence.
ACCESSION   DASQQQ010000042 DASQQQ010000000
VERSION     DASQQQ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388470"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..2043
                     /locus_tag="VE646_02100"
     CDS             106..2043
                     /locus_tag="VE646_02100"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HZD78819.1"
                     /translation="MRGRRRRTVAAGWLASVGIASALAVGAAPTTVEAQKSLVFEEFA
                     SEIQVRPDADIIVTETLRPRFEGSWNGIERVLELRPPADYGVAYLLEVRLIGATDGAG
                     HPLRTEETRLDRDTRRFRVWVPEATDRTATVILRYQVGNALGFFAPDTTGLTLDELYW
                     QVTGTTSDVPILSARARVVLPAGAQPKQAAAYVGGAGSTRQTRVILGEGSASVPAVGP
                     LDPGEGLTVAVGWAAGAVTRPAGAAAPTTAAQSGGRGRVGAGGTAPGGPWSPLALWPV
                     LLPLLAFWLAYRAWRRRGRDPAERAITVRWEPPPDISPAEAGTLVDHRADMPDIVATL
                     VDLAVRGVVVIEEREKGGFLSFGKDYAFHLMKPSPQWDDLADHEREFLNGLFERTTRR
                     ERVKAVGEKTGGFLKGVVETFTGEPAEEGAPDGAIDSVLLSDLKNEFYRTLPDIRDAI
                     YRQLVLKGHYLRRPDTVRRRWVLLSMLCVGIGSIGFAGAAGGAGNGRVMALALIVGGF
                     ASALIVGVFGWLMPARTEQGARTREAALGFKRFLERVETPRYRRMITSPKQFEEYLPF
                     AMAFGCEEQWAKAFEGLLTEPPEWYLGTHGAFRASAFASDLGTLSTAAASTLSSSPSS
                     SGSGGGGSVGGGGGGGGVGGF"
     gene            2158..>2414
                     /locus_tag="VE646_02105"
     CDS             2158..>2414
                     /locus_tag="VE646_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78820.1"
                     /translation="MGSFATNIRRILLALFVLGVIAATVELLLLGHTESTTQQIPLFT
                     LALAGVAAATVAVWPRRWVVRGFQLVGAVMIGVGGLGLYMH"
BASE COUNT          323 a          828 c          904 g          359 t
ORIGIN      
        1 tcagttcccc tcgaacatcg tggcgaacgc ggcgcggatc tgggagcggg agttcttcga
       61 gatcgaggcg ccggagcagc gcgaggcgcc gcgggtggag ttctgatgcg cggccggcgc
      121 cgccgcaccg tggcagccgg ctggctcgcg tccgtcggta tcgcatcggc gctcgcggtc
      181 ggggccgcgc ccaccaccgt cgaggcccag aaatcactgg tcttcgagga gttcgcgtcc
      241 gagatccagg tccggccgga cgcggacatc atcgtcaccg agacgctccg accgcgcttc
      301 gaagggtcct ggaacgggat cgagcgggtg ctcgagctcc ggccgccagc ggactacggc
      361 gtggcctacc tcctggaggt gcggctcatc ggcgccacgg acggcgcggg gcatccgctc
      421 cgcacggagg agacccggct cgaccgcgac acccgccgct tccgggtctg ggtccccgaa
      481 gcgacggatc gcaccgccac ggtcatcctc cggtatcagg tcgggaacgc cctcggcttc
      541 ttcgccccgg ataccacggg cctcacgctc gacgagctct actggcaggt gaccggaacg
      601 acctcggacg tgccgatcct cagcgcgcgg gcgcgggtgg tcctgcccgc cggggcgcag
      661 ccgaagcagg cggcggcata cgtgggcggc gccgggtcga cgcggcagac ccgagtgatc
      721 ctgggcgagg gctccgcctc cgtgccagcg gtggggccgc tcgaccccgg agaagggctg
      781 accgtggccg tggggtgggc ggccggcgcc gtcacgaggc cggccggagc ggcggccccc
      841 accacggccg ctcagagcgg aggccggggc cgtgtggggg ccggcgggac cgcacccggc
      901 gggccgtgga gcccgctcgc cctctggccg gtgctgctcc cgctcctggc gttctggctc
      961 gcgtaccgcg cgtggaggcg gcggggtcgg gatcccgcgg aacgggcgat caccgtccgc
     1021 tgggagccgc cgccggatat cagcccggcg gaggccggca ccctggtcga tcatcgcgcg
     1081 gacatgcccg acatcgtcgc caccctcgtc gacctcgccg tccggggcgt ggtcgtgatc
     1141 gaggagcggg agaagggggg gttcctctcg ttcggcaagg attacgcgtt ccacctgatg
     1201 aagccttcgc cccaatggga cgacctcgcg gatcatgagc gggagttcct gaacggtctc
     1261 ttcgagcgga cgacccgccg ggagcgggtg aaggcggtgg gtgagaagac gggcgggttc
     1321 ctgaagggcg tggtcgagac gttcaccggt gagccggccg aggagggagc gcccgacggt
     1381 gccatcgaca gcgtcctgct ttccgatctg aagaacgagt tctacaggac gctccccgac
     1441 atcagggacg ccatttaccg ccagctggtg ctgaaaggcc attacctccg ccgtcccgac
     1501 accgtgcgcc ggcgctgggt cctgctgagc atgctctgcg tgggaatcgg gtcgatcgga
     1561 ttcgccggcg cggccggcgg cgcggggaac ggcagggtca tggcgctggc gctgatcgtc
     1621 ggcgggttcg cgagcgcgct catcgtgggc gtattcggct ggctgatgcc ggcgcggacg
     1681 gagcagggcg cacgaacccg ggaagcagcc ctcgggttca agcgcttcct cgagcgggtc
     1741 gagaccccgc gctaccgacg catgatcacg tcgccaaagc agttcgagga atacctcccg
     1801 ttcgccatgg ccttcggctg cgaggaacag tgggcgaagg cgttcgaggg cctgctcacg
     1861 gagccaccgg agtggtatct cggcacccac ggcgcgttcc gagcgtccgc gttcgccagc
     1921 gacctgggga cgctctccac cgccgcagcc tccaccctct cctcctcgcc ctcctcctcc
     1981 gggtccggcg gtgggggcag cgtgggcggt ggcgggggag gcggaggcgt gggaggattc
     2041 tgacccgggc gccggtgcct ggcgctccgt gccgctgccg cccaccgcgg ggccgtgccc
     2101 ggtcgaccga ccggtatgcg acgtgcatgg tgccaggttc cactcacagc cacccgaatg
     2161 ggctctttcg caaccaacat acgccggatc ctgctcgccc tcttcgtgct gggcgtgatt
     2221 gcggccacgg tcgagctgct gctcctgggg cacaccgaat ccaccaccca gcagatcccg
     2281 ctgttcacgc tggcgctggc gggcgttgcg gcggccacgg tggcggtctg gccgcggcgg
     2341 tgggtggtgc ggggattcca gctcgtgggg gccgtgatga tcggcgtggg cggcctcggc
     2401 ctctacatgc acta
//
LOCUS       DASQQQ010000043         8620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_1401, whole genome shotgun sequence.
ACCESSION   DASQQQ010000043 DASQQQ010000000
VERSION     DASQQQ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8620
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="VE646_02110"
     CDS             complement(<1..1212)
                     /locus_tag="VE646_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78821.1"
                     /translation="MAVAALGQLAAFAVYVASGSGSALTFAKLGWFYFGWFHHVGLVA
                     ELPDLALGRGASQSIGGALPGGTSISYTAGLALMLVTLVAGGLLFLAGRAAAGAAGGG
                     PAARMLHGMKIAPAYAVPALLISLAVSIDTAIPANAFVGGRVRVHESTWQSFVFPFAI
                     ALIAGALGGSSTVGPAVRRERARVRRTLGSVAGGVRMFLLAIVLAFVGMLVLAAVRPD
                     ATKAYLTAVSEPPADQTAVVVAHHILLLPNQSMWVLVPAMGSCDGAYGSGFSAPFLCY
                     WKYPQTVKTSLISPQAGLGDLASPVDARFGTAPWWSFLFLLVPLVSVLAGGWHGARHG
                     GAETAAEGGLLGAAAGVVFAVFVAAGIWLSSVSAGLNASVLGMSTGGSVRVGPDLLSG
                     TILALLWGVVGG"
     gene            complement(1302..2171)
                     /gene="sucD"
                     /locus_tag="VE646_02115"
     CDS             complement(1302..2171)
                     /gene="sucD"
                     /locus_tag="VE646_02115"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HZD78822.1"
                     /translation="MAILVDGSTRVVVQGLTGREGHFHALRNRDYGTQVVAGTSPGKA
                     GRDVEGIPVFDTVADAVAETGANTSLIFVPARFAPEAIYEAADSDLRLVVCITEGIPV
                     RDMARVGSYLRGKASALVGPNCPGLISPGRSNVGIIPGEICSPGRVGLVSRSGTLTYQ
                     IVHELTQRGIGQSTCVGMGGDPVHGVGFIECLERFERDPETDLVVMIGEIGGDDEERA
                     ARFVGEHMSKPVVGYIAGFTAPPGKRMGHAGAIVTGSRGTAQAKAEALEAAGVKVGRT
                     PTEVAEQAEAGLA"
     gene            complement(2171..3316)
                     /gene="sucC"
                     /locus_tag="VE646_02120"
     CDS             complement(2171..3316)
                     /gene="sucC"
                     /locus_tag="VE646_02120"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018505063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HZD78823.1"
                     /translation="MDLYEHQGKELFAKHGIPVPRGIVSQTPEAARAATEILGGASVV
                     KVQVQVGGRGKGGGVVLADTPDQAEEAAGRLLTEGFQRRRVNRVLVEERLPIEREFYA
                     AITLDRSSGTDLAMVAAEGGVEIEELAATKPEALRRLHVDPEIGIHPYHLRGLTGALP
                     PEARDLVENVLAGLYDVLTTEDATLVEVNPLVLLTDGRVFALDAKVTIDDNALFRHPE
                     LEMLKSSFPIDPVEFRAREAGLQYVKLEGEVGIIGNGAGLVMSTLDVVQRAGSAAANF
                     LDIGGGAGADTMATSLEVILSDPSVRSVLVNIFGGITRCDLVAEGILGALDRIESRVP
                     IVVRLDGTNATEGRSILARAAHPTIVPAETMLEAAQTAARLAHEDAA"
     gene            complement(3316..3723)
                     /locus_tag="VE646_02125"
     CDS             complement(3316..3723)
                     /locus_tag="VE646_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012015020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HZD78824.1"
                     /translation="MPERYRVVVAKPGLDGHDRGVKVVARALRDAGFEVVYTGLRQTP
                     EQIVEAVIQEDADAVGLSCHSGAHMTLFPKVVHRLRERGAADVVVFGGGIIPSDDIPK
                     LAAEGIEQIFTPGATTGEIVTWLNERLATKRAS"
     gene            3797..3961
                     /locus_tag="VE646_02130"
     CDS             3797..3961
                     /locus_tag="VE646_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78825.1"
                     /translation="MGKKRIRFRPPGLKPTTKVGTLGPYRGRLGLAWVIAPIVAGLII
                     LAAGYVALFR"
     gene            3997..4476
                     /locus_tag="VE646_02135"
     CDS             3997..4476
                     /locus_tag="VE646_02135"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZD78826.1"
                     /translation="MRWDEFEAACPEIATLARERFAADQLVLVGTIRADGSPRISPNE
                     VDLAAERLLLGMMWRSRKALDLLRDPRVVVHSVPTDRFNTGGDVKLYGRVVDEQDPGV
                     RRAFREEIRRRIDWAPDEPEYHLFSLDVSAAGYTVFGEGSHALAWDPARSLRRLALR"
     gene            complement(4504..6693)
                     /gene="pcrA"
                     /locus_tag="VE646_02140"
     CDS             complement(4504..6693)
                     /gene="pcrA"
                     /locus_tag="VE646_02140"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HZD78827.1"
                     /translation="MTEPSPLLGSLNPVQREAVLHTEGPVLIVAGAGSGKTRALTHRI
                     AYLIREQQTSPYAILAITFTNKAAREMADRVEELLGGRVARGMWILTFHSACARILRR
                     EHERLGLPSTFTIYDENDTERLIAQVLRDLNIDNKRYPPRAMAAGIGRAKDHVLSARD
                     FQAAAGNVYEQTVARVYVGYEERKHAAGALDFDDLITEAVRLFRQQPQVLEHYQERFR
                     YLLIDEYQDTNRAQYQLANLLAAKYRNICVVGDADQGVYSWRGATIQNLLDFERDYPD
                     AAVFLMEQNYRSTENILAVANALIEHNLQRKPKSLCTETEGGELAVRYRADNEHEEAL
                     FVAEETDRLVDEEPYRYADIAIFYRTNAQSRVLEDVFMRAGLPYRVVGGVRFYQRKEI
                     KDVLAYLRLLMNPQDLISARRVVNAPKRGIGDQTVAALESFALAEEVPFLEACRRVEE
                     IAVLGARARGAVSGFVQIMDRLAEALASGAGPARMVEAAATESGYLLELETERTIEAE
                     GRIENLRELGGVAAEHEARFPHEGLAGFLEQVALVSEQDEYDQEASSVTLMTLHNAKG
                     LEFPVVFIIGMEDGVFPHYRSMGDAAELEEERRLAYVGITRAKERLYLTHAWSRTLFG
                     TTSYNPPSRFLAEIPSELVRSIDEEGQEGIAIGSGGGGSAIREAVTGRRPSVEVAAGD
                     TVLHDKWGEGVVLTVSGAGSDAEATVIFEDVGQKRLLLAYAPLKKVG"
     gene            6833..7495
                     /locus_tag="VE646_02145"
     CDS             6833..7495
                     /locus_tag="VE646_02145"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HZD78828.1"
                     /translation="MDSASADPAGAWPRRGSPPRRRRRGRILFAIGAALIAASLAIGS
                     YLGWQLWGTGLATQRWQSQLRKGFEKQIDTQVATPTAGSADPRVVLPGQAVAILQIPR
                     IGLDMVVVEGTGIEDLKKGPGHYTDTAYPWQDHGRVGIAGHRTTYLHPFFHLDQVRPG
                     DRIILQTRYGTYDYEVTRVFVTLPDDGSVLEQTRRPTLVLTTCEPRYSASHRLIVDAV
                     RT"
     gene            7557..8075
                     /locus_tag="VE646_02150"
     CDS             7557..8075
                     /locus_tag="VE646_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HZD78829.1"
                     /translation="MKKVVVALFVTGLVGLAGTVPASAQAYGGQPGLSCTNDTVTGDH
                     LTPNTTPTIYVDGHPSGQAQVGRDGSFSFTLPASAGSGRHTVTVQGISARAVCSIPSE
                     VAGETVQKGKPAPETTTPPGGTAFTGASVITPSLIAVGLLLLGVASIVAGRRRRRIRA
                     GAGDGSGDGPAS"
     gene            8245..>8620
                     /locus_tag="VE646_02155"
     CDS             8245..>8620
                     /locus_tag="VE646_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78830.1"
                     /translation="MLLGSVVLSLIPALSSVRRLREGRVALAEARSSLLDADAGAASD
                     AFARARLAFTDASGNANNVLTDLPSMLPLLGRTYEALRTLAGSGRQIAVAGEQVAGAV
                     GSLPGGPAALGPHHGRLPLGRYE"
BASE COUNT         1209 a         3226 c         2980 g         1205 t
ORIGIN      
        1 cccgcccacc acgccccaga gcagcgcgag gatcgtgccg ctcagcaggt cgggccccac
       61 ccggaccgag cccccggtgg acatgcccag caccgaggcg ttcagtcccg ccgacaccga
      121 ggagagccag atccccgccg ccacgaacac ggcgaacacc acgccggcgg cggcgccgag
      181 caacccgccc tcggcggccg tctcggcccc accgtgacga gcgccgtgcc aacctccggc
      241 caggacggag accagcggga ccagcaggaa caggaacgac caccacggcg cggtgccgaa
      301 gcgcgcgtcc accggtgagg ccagatcccc caagccggcc tgaggcgaga tgaggctggt
      361 cttcaccgtc tgcgggtact tccagtagca caggaacgga gcggagaacc cgctgccgta
      421 ggccccgtcg caggagccca tcgccgggac cagcacccac atcgactggt tcggcaggag
      481 caggatgtgg tgggcgacga ccacggcggt ctgatcggcc ggtggttcgg agaccgcggt
      541 gaggtaggcc ttggtggcgt ccggtcggac cgccgcgagc accagcatcc cgacgaaggc
      601 caggacgatc gccagcagga acatccggac cccaccggcc accgacccga gcgtccgtcg
      661 cacccgggca cgctcccggc gcaccgccgg tcccacggtg gacgatccac ccagcgcccc
      721 ggcgatcagc gcgatggcga aggggaacac gaacgactgc caggtcgact cgtgcacccg
      781 gacccggccg ccgacgaagg cgttggccgg gatcgccgtg tcgatcgaga cggccagcga
      841 gatcagcagc gccggcaccg cgtaggccgg cgcgatcttc atcccgtgga gcatgcgcgc
      901 ggcggggccc ccaccggccg caccggccgc ggctcgcccc gccaggaaga gcagccctcc
      961 cgccaccagc gtgaccagca tgagggcgag gcccgccgtg taggagatgc tcgtgccccc
     1021 cggcaacgcc cccccgatcg actgcgatgc cccccgcccc agggccaggt cggggagctc
     1081 ggccaccagc cccacgtggt ggaaccagcc gaagtagaac caacccagct tcgcgaaggt
     1141 gagggccgag cccgagccgg acgcgacgta gacggcgaac gccgcgagct ggccgagcgc
     1201 cgcgaccgcc acgaatgcca ggagcccccg gatcagagct gtgggccacc ccgcggaaag
     1261 ggcgtcccca ctccgggcgg ccggcggagc ggggggctcc atcatgccag cccggcctcc
     1321 gcctgctccg ccacctcggt gggggtccgg cccaccttca cccccgccgc ctccagggcc
     1381 tcggccttgg cctgggccgt gccccgtgat ccggtgacga tggcccccgc gtgacccatg
     1441 cgcttgccgg gaggcgccgt gaaccccgcg atgtagccca cgaccggctt gctcatgtgc
     1501 tcgcccacga agcgcgcggc gcgctcctcg tcgtcgcccc cgatctcgcc gatcatcacc
     1561 accagatcgg tctcggggtc ccgctcgaac cgttccaggc actcgatgaa ccccaccccg
     1621 tggacggggt cgccccccat ccccacgcag gtggactggc cgatcccgcg ctgcgtgagt
     1681 tcgtgcacga tctggtaggt gagcgttccg gaccgagaga ccagcccgac cctgcccggc
     1741 gagcagatct ccccggggat gatgcccacg ttggaccggc ccggggagat gagcccgggg
     1801 cagttcggcc cgaccagcgc ggaggccttg ccccgcaggt agctccccac ccgggccatg
     1861 tcccgcacgg ggatcccctc ggtgatgcac acgaccaggc gcaggtcgga atcggccgcc
     1921 tcgtagatcg cctcgggggc gaagcgggcc ggcacgaaga tcagcgaggt gttcgccccg
     1981 gtctcggcca cggcgtcggc cacggtgtcg aacacgggga tcccttcgac gtcccgtccg
     2041 gccttgcccg ggctggtgcc ggccaccacc tgcgtcccgt agtcgcggtt gcgcagggcg
     2101 tggaagtgcc cctcccggcc ggtgagcccc tggaccacca ccctggtcga tccgtcgacc
     2161 aggatcgcca tcaggccgcg tcctcgtgcg ccagccgggc cgccgtctgc gctgcctcga
     2221 gcatcgtctc cgccggcacg atcgtgggat gcgccgcccg ggcgaggatg ctccggccct
     2281 cggtggcgtt ggtcccgtcc agccgcacca cgatgggcac ccgggactcg atccggtcca
     2341 gcgcgcccag gatgccctcg gccaccagat cgcagcgcgt gatccccccg aagatgttca
     2401 ccagcacgga gcggacgctc gggtcggaca ggatcacctc cagggaggtg gccatggtgt
     2461 ccgcgccggc cccgccgccg atgtccagga agttggccgc ggcggacccc gcccgctgga
     2521 ccacgtccag cgtggacatg accagcccgg ccccgttgcc gatgatcccc acctcgccct
     2581 cgagcttgac gtactggagc cccgcctccc gggcccggaa ctccaccggg tcgatgggga
     2641 aggacgactt cagcatctcg agctcggggt ggcggaacag cgcgttgtcg tcgatcgtga
     2701 ccttggcgtc gagggcgaac acccggccgt cggtgagcag gaccagcggg ttcacctcga
     2761 ccagggtggc gtcctcggtg gtcagcacgt cgtacaggcc ggccagcacg ttctccacca
     2821 ggtcccgggc ctcgggcggg agcgccccgg tcagcccccg gaggtggtag gggtggatcc
     2881 cgatctcggg gtcgacgtgc aaccgccgca gggcctcggg cttggtggcg gcgagctcct
     2941 cgatctccac gccgccctcc gcggccacca tcgccaggtc ggtgcccgag ctccgatcca
     3001 gcgtgatcgc ggcgtagaac tcgcgctcga tcggcagccg ctcctccacc agcacgcggt
     3061 tcacccggcg gcgctggaag ccctcggtca gcaaccgccc ggccgcctcc tccgcctgat
     3121 ccggcgtgtc ggccagcacc acgccgccgc ccttgccgcg gccgcccacc tgcacctgga
     3181 ccttgaccac ggaggcgccg ccgaggatct cggtggccgc ccgggcggcc tccggcgtct
     3241 gggacacgat cccccgcggc acggggatgc cgtgcttggc gaagagctcc ttgccctggt
     3301 gctcgtacag gtccatcagc tggcccgctt cgtcgccagc cgctcgttca gccaggtcac
     3361 gatctcgccg gtggtggcgc cgggggtgaa gatctgctcg atgccctctg ccgcgagctt
     3421 ggggatgtcg tcgctcggga tgatcccccc tccgaacacg acgacgtcgg ccgctccgcg
     3481 ctcgcggagc cggtgcacga ccttggggaa cagcgtcatg tgcgcgccgg agtggcagga
     3541 gagcccgacg gcgtcggcgt cctcctggat cacggcctcc acgatctgct cgggggtctg
     3601 gcgcaggccc gtgtagacca cctcgaaccc ggcgtcgcgg agcgctcggg ccaccacctt
     3661 gaccccgcgg tcgtggccgt cgagccccgg cttggcgacc accacccgat agcgctccgg
     3721 catgcggccc atcataacca acgcccatcc tcgtcccggc gctcggcgtt ccggcggagc
     3781 gcggtacgct ggatgggtgg ggaaaaagcg catccggttc cgcccgccgg gcctgaagcc
     3841 caccacgaag gtcggcacgc tcgggccgta ccggggccgt ttgggcctgg cctgggtgat
     3901 cgcgcccatc gtggccggcc tgatcatcct ggccgccgga tacgtcgccc tcttccgctg
     3961 agccagggag ccgtcggatg accgggaggg gatcgtttgc gctgggatga gttcgaggcc
     4021 gcctgtcccg agatcgccac gctggcccgt gagcggttcg ccgccgacca gctggtgctg
     4081 gtggggacga tccgcgccga cgggagcccc cgcatcagcc cgaacgaggt cgatctcgcg
     4141 gccgagcggc tcctgctcgg gatgatgtgg cggtcccgga aggccctgga cctcctccgc
     4201 gacccgcgcg tggtggtgca cagcgtaccg accgaccggt tcaacaccgg cggcgacgtc
     4261 aagctgtacg gccgcgtggt ggacgagcag gaccccggcg tgcgtcgggc cttccgggag
     4321 gagatccggc ggcggatcga ctgggccccg gacgagcccg agtaccacct gttctccttg
     4381 gatgtgagcg ccgccgggta cacggtcttc ggcgagggat cgcacgcgct ggcctgggac
     4441 cccgctcgga gcctccgacg cctggcgctg cgctgatcgc gaacggaacc tgtggaagga
     4501 gcctcagccc accttcttca gcggggcgta cgcgaggagc aggcgcttct gccccacgtc
     4561 ctcgaagatc accgtggcct cggcgtccga gcccgcgccc gagaccgtga ggaccacgcc
     4621 ctcgccccac ttgtcgtgca gcaccgtgtc accggcggcc acctccacgg acggccgccg
     4681 cccggtcacc gcttcacgga tcgccgagcc acctccgccc gagccgatcg cgatcccctc
     4741 ctggccctcc tcgtcgatgc tgcggaccag ctcgctgggg atctcggcca ggaaccggga
     4801 gggcgggttg tacgaggtcg tgccgaacag ggtccggctc caggcgtggg tcaggtacag
     4861 gcgctccttg gcccgggtga tccccacgta ggccagccgc cgctcctcct ccagctccgc
     4921 cgcgtcgccc atcgagcggt agtgggggaa gacgccgtcc tccatcccga tgatgaacac
     4981 caccgggaac tccaggccct tcgcgttgtg cagggtcatg agcgtgaccg aggacgcctc
     5041 ctggtcgtac tcgtcctgct cggagaccag ggccacctgc tccaggaacc cggccagccc
     5101 ttcgtggggg aagcgggcct cgtgctcggc ggccacgccg cccagctccc ggaggttctc
     5161 gatccggccc tcggcctcga tcgtgcgctc ggtctcgagc tcgagcaggt agcccgactc
     5221 ggtggcggcc gcctccacca tccgcgccgg gccggcgccc gatgcgagcg cctcggccag
     5281 gcggtccatg atctgcacga acccggacac ggccccccgg gcacgagccc ccagcaccgc
     5341 gatctcctcc acccgccggc atgcctccag gaaggggacc tcctcggcca gggcgaagga
     5401 ctccagggcc gccacggtct gatcgccgat cccgcgcttc ggcgcgttca ccacccggcg
     5461 ggcgctgatc aggtcctgcg ggttcatcag caatcgcagg taggccagga cgtccttgat
     5521 ctccttgcgc tggtagaagc ggacgccccc caccaccctg tagggaaggc cggcccgcat
     5581 gaacacgtcc tccagcacgc ggctctgggc gttggttcga tagaagatcg cgatgtccgc
     5641 gtaccggtag ggctcctcgt cgaccagccg gtcggtctcc tcggcgacga acagtgcctc
     5701 ctcgtgctcg ttgtccgccc ggtaccggac cgcgagctcc ccgccctcgg tctcggtgca
     5761 caggctcttc ggcttgcgct ggaggttgtg ctcgatcagc gcgttggcca ccgccaggat
     5821 gttctcggtg gagcggtagt tctgctccat caggaacacg gcggcgtccg ggtagtcgcg
     5881 ctcgaagtcc aggaggttct ggatggtggc cccccgccag gagtagacgc cctggtccgc
     5941 gtcacccacc acgcagatgt tgcggtactt ggcggccagc aggttggcca actggtactg
     6001 ggcccggttc gtgtcctggt actcgtcgat cagcaggtac cggaaacgct cctggtagtg
     6061 ctccaggacc tggggctgct gccggaacag ccggaccgcc tcggtgatca ggtcgtcgaa
     6121 gtcgagcgcc cccgcggcgt gcttgcgttc ctcgtagccc acgtacaccc gggccacggt
     6181 ctgctcgtac acgttcccgg ccgccgcctg gaagtcccgg gccgagagca cgtggtcctt
     6241 ggcccggccg atccccgccg ccatggctcg gggcgggtac cgcttgttgt cgatgttcag
     6301 gtcccgcagg acctgggcga tcagccgctc ggtgtcgttc tcgtcgtaga tcgtgaaggt
     6361 cgagggcaag cccagacgct cgtgctcgcg ccgcaggatc cgggcgcagg ccgagtggaa
     6421 ggtcaggatc cacatccccc gggccacacg gcctcccagg agctcctcca cccggtcggc
     6481 catctcccgc gccgccttgt tcgtgaaggt gatggcgagg atcgcgtagg ggctcgtctg
     6541 ctgctcccgg atcaggtagg cgatgcggtg cgtgagcgcc cgggtcttgc ccgatccggc
     6601 gccggcgacg atcagcaccg ggccctccgt gtgcagcacg gcctcgcgct ggaccgggtt
     6661 gagggacccg agcagggggg acggttcggt cacggatgcg attgtaggga ccgcgcccga
     6721 cgacggaccc aggcgccgca gccgtgctcg agcgctcgca ctaggtcccc cggcgaggaa
     6781 acggccggac ggcgggggcg gcaacccgga gggtcgtgcc acactggagg ggatggacag
     6841 cgcttccgcc gatcccgccg gcgcctggcc gcgccggggc tcgcctcccc gccgccggcg
     6901 gcgcgggcgc atcctgttcg cgatcggggc ggcgctgatc gcggcctccc tcgcgatcgg
     6961 ctcctacctg gggtggcagc tgtggggcac cggcctcgcc acgcagcgct ggcagagcca
     7021 gctgcggaag gggttcgaga agcagatcga cacccaggtc gccacgccca cggccggctc
     7081 cgccgacccg agggtggtcc tgcccggcca ggccgtggcg atcctgcaga tccccaggat
     7141 cgggctggac atggtggtgg tggaggggac gggcatcgag gatctgaaga aggggccggg
     7201 tcactacacc gacaccgcgt acccttggca ggatcacggg cgggtgggga tcgccgggca
     7261 ccggaccacc tacctgcatc cgttcttcca cctggaccag gtccgccccg gcgatcgcat
     7321 catcctgcaa acgaggtacg gaacctacga ctacgaggtg acgcgcgtgt tcgtcacgct
     7381 gccggatgat ggttcggtac tggaacagac ccggcgaccg accttggtcc tgacgacatg
     7441 cgaaccgagg tactccgcct cccatcgcct gatcgtggac gcggtccgaa cctgaaccgc
     7501 ggtctgcccg ccgcaccggt cctggacgga ccggtccgca cgaaacgagg tgccagatga
     7561 agaaggtggt cgttgcgctg ttcgtcaccg gcctggtcgg cctggccggg acggttccgg
     7621 cctcggcgca ggcctacggc gggcagcccg ggctgagctg cacgaacgac acggtgacgg
     7681 gtgaccacct cacccccaac accaccccca cgatctacgt ggatgggcac ccgtcgggcc
     7741 aggcccaggt cggccgggac ggcagcttct ccttcaccct gccggcgtcg gccggctccg
     7801 ggcgccacac cgtcacggtg cagggcatca gcgcgcgcgc cgtgtgctcc atcccctcgg
     7861 aggtggccgg cgagacggtc cagaagggca agcccgcccc ggaaaccacg accccgccgg
     7921 gcggcaccgc cttcaccggg gcctcggtca tcacccccag cctgatcgcg gtcgggctgc
     7981 tcctgctggg ggtggccagc atcgtggccg gtcgtcggcg ccgccggatc cgcgccggtg
     8041 ccggcgatgg ctcgggggac ggtcccgcct cctgaccggc agcgctcggc atcccgccac
     8101 gatcttcggt cgcgctccgc gtgaggaggg ctcggtcccc tgatcgccca agggccgccc
     8161 caccgggggg acgacgcggg atccggccct cggcggggcc gctggtggcg tgcctccgcc
     8221 ttcctgatcg gcgcggtcct ggccgtgctc ctggggtcgg tggtcctttc gctgatcccc
     8281 gcgctgagca gcgtgcggcg attgcgcgag gggcgcgtcg cgctggccga ggccagatcc
     8341 tcgctcctgg atgcggacgc gggcgccgcc tcggacgcgt tcgcccgggc ccggctcgcc
     8401 ttcaccgacg cgtccggcaa cgccaacaac gtcctgaccg acctgccctc catgctcccg
     8461 ctgctgggca ggacctacga ggcgctccgg accctggccg gatcggggcg gcagatcgcg
     8521 gtggccgggg agcaggtggc cggcgccgtc ggttccctcc ccgggggccc tgcggccctg
     8581 gggccccacc acgggcggct gcccctgggc cggtacgagc
//
LOCUS       DASQQQ010000044         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_278821, whole genome shotgun sequence.
ACCESSION   DASQQQ010000044 DASQQQ010000000
VERSION     DASQQQ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278821"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..>2286)
                     /locus_tag="VE646_02160"
     CDS             complement(133..>2286)
                     /locus_tag="VE646_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HZD78831.1"
                     /translation="GWMGVGDAHRPPRAAVRVRRLPTAPGRAWDRFIDAVSTRAAYRP
                     WRFVAIVMVTTFLLLVGMLPVAFGARTALDFVHRRFLGSINTPLHIPPMAERSTILDS
                     HGSVLQHVYLNFDRKLVPLSAYPRLTRRSVLAIEDHAFYQHGAVDIRSTVRALLADLA
                     AGRIVQGGSTISQQLVKDTITGDARTLARKYHEALDAIRLEHTYPKRRILSMYMNEVY
                     LGNGVYGFAAAARYYFAEQPGQLTLPQSALLAGMIESPTAYDPLVHPKAARGRRGAVL
                     TSLLDLGWITRSRFQRAMATPVRLSAQGRASVHSGPTSFWTQYVIHSFLSDPAFGATP
                     RDRRRALFQGGLRITTTLDPSMQREAERAIAQRMTGPGLPQSALVSIAPGSGAIRAMA
                     VGNWPFSRHQYDLAVDPGGGRTAGSSFKAFTLAAALEQGISPNAVYNGDSPKTIPHCG
                     GGDTWTLHNAEPGSGNYPLWLATADSVNVVFAQVIDQVGPAAVARVAHRMGITSPLDP
                     VCSLTLGTSPVSPLQMGSAYATLANDGVHCMPYPIAMVQDRTGKTIYRARPQCQRAIP
                     ASVAEEETAMLENVIRMGTGTAANIGRPAAGKTGTGQDYQDAWFVGFVPQLATAVWVG
                     YASAEVPMSDVPGYGHGFGATLAAPIWHDFMAAATGSVPARSFAPAPIPFGSSSSAPT
                     SSPSIGASPTPSPSPSPSSPAPSPSPSSPTASPSP"
BASE COUNT          348 a          768 c          851 g          319 t
ORIGIN      
        1 gagacggagc tgctggccat caccggggcc gtcgcggccg gacggatcga tgatgcggtc
       61 acccgcgcgg agcgcttggc cgagcgcctg gagcacccct cgtcccgagc ggtccgctga
      121 tcggcgcgag cgtcacggcg acggcgacgc ggtcgggctc gaaggtgatg gcgacggcgc
      181 ggggctcgaa ggcgatggcg acggcgacgg cgtggggctc gcgccgatcg agggggacga
      241 ggtcggggcc gagctcgagc tcccgaaggg aatcggcgcc ggcgcgaacg agcgcgcggg
      301 cacggaaccg gtggcggcgg ccatgaagtc gtgccagatc ggagcggcca gggttgcccc
      361 gaacccgtgg ccgtaccccg ggacgtcgct catcggcacc tccgcgctgg cgtaccccac
      421 ccagaccgcc gtggccagct gagggacgaa gcccacgaac caggcgtcct ggtagtcctg
      481 accagtgccg gtcttgccgg ccgccggacg cccgatgttc gccgccgtcc ccgtgcccat
      541 ccggatgacg ttctcgagca tggccgtctc ctcctcggcc accgaggccg ggatggcgcg
      601 ctgacactgg ggccgagctc ggtagatcgt cttgccggtc cggtcctgca ccatggcgat
      661 cgggtacggc atgcaatgca cgccgtcgtt ggccagggtg gcgtaggccg agcccatctg
      721 gagcggtgag acgggcgatg tgccgagcgt cagcgagcac acggggtcga gcgggctggt
      781 gatgcccatc cggtgggcaa cccgggccac cgcggccggc cccacctgat cgatgacctg
      841 ggcgaacacc acgttgaccg aatcggccgt ggccagccac agggggtagt tgcccgagcc
      901 cggctcggcg ttgtgcaggg tccaggtgtc ccccccgcca caatgcggga tcgtcttcgg
      961 cgagtcaccg ttgtagaccg cgttgggcga gatgccctgc tccagcgcgg cggccagcgt
     1021 gaaggccttg aacgagctgc ccgccgttcg cccgccgccc gggtccaccg ctaggtcgta
     1081 ctgatgccga ctgaagggcc agttccccac cgccatggcc cggatggcac cgctgccggg
     1141 agcgatgctc accagggcgg actgggggag gcccggcccg gtcatccgct gggcgatggc
     1201 tcgttccgcc tcgcgctgca tcgaggggtc cagggtggtg gtgatccgca ggcctccctg
     1261 gaagagtgcc cgcctccgat cgcggggcgt ggctccgaag gccggatccg agaggaagct
     1321 gtggatcacg tactgggtcc aaaaggacgt cggtcccgag tgcacgctcg cgcgcccctg
     1381 cgccgagagg cggaccggtg tcgccatggc ccgctggaac cgggaccggg tgatccagcc
     1441 gagatccaac aggctggtaa gcacggcacc ccgccggcca cgagccgcct tcgggtgcac
     1501 cagcgggtcg taggcggtgg gggactcgat catccccgcc agcagtgccg actgcggcag
     1561 cgtgagctgg ccgggctgct cggcgaagta gtagcgggcc gccgcggcga acccgtagac
     1621 gccgttgccc aagtagacct cgttcatgta catcgagagg atcctgcgct tggggtaggt
     1681 atgctccagc cggatcgcgt ccagcgcctc gtggtacttg cgggccaggg ttctcgcatc
     1741 gccggtgatc gtgtccttga ccagttgttg ggagatcgtg gaaccgccct gcacgatccg
     1801 cccggccgcc aggtccgcca acaacgctcg cacggtcgac cggatgtcga ccgctccgtg
     1861 ctggtagaag gcgtggtcct cgatggccag caccgagcgc cgggtcagcc gggggtacgc
     1921 ggagagcggc acgagcttgc ggtcgaagtt cagatagacg tgctggagca ccgagccgtg
     1981 ggaatcgagg atcgtggatc gctcggccat cggtgggatg tggagcggtg tgttgatcga
     2041 gccgaggaac cggcgatgga cgaagtccag ggccgtccga gcgccgaagg cgaccgggag
     2101 catccccacc agcagcagga aggtggtgac catcacgatg gccacgaacc tccaaggccg
     2161 gtacgcggcc cgggtggaga ccgcgtcgat gaaccggtcc cacgcccggc cgggggcggt
     2221 gggcagccgc cgcacccgta cagccgctcg cgggggccgg tgcgcgtccc cgacacccat
     2281 ccaccc
//
LOCUS       DASQQQ010000045         7301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_251812, whole genome shotgun sequence.
ACCESSION   DASQQQ010000045 DASQQQ010000000
VERSION     DASQQQ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251812"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1508
                     /gene="gatA"
                     /locus_tag="VE646_02165"
     CDS             72..1508
                     /gene="gatA"
                     /locus_tag="VE646_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HZD78832.1"
                     /translation="MADLLELSVHEALERIEAREVGRDELLSTYVAAAAGDELNAYLW
                     RAEPGKPGCAGRGEGPLAGIPVAVKDLFCTEGVETTAGSRILQGHRPLYTATAVRRLT
                     DAGARVLGKTNMDEFAMGSSNENSAYGPVRNPWDLGTVPGGSSGGSAAAVAAGEAVWA
                     LGTDTGGSVRQPAALCGVVGLKPTYGLISRYGLIAFASSLDTVGTFTRSVRDAAVLLS
                     TIAGKDPLDATSLDEPRRDYAGDLGQGVAGLRVGVVRETFGQGVEPDVASAVRSAVDR
                     FAALGAEVGEASLPHADYALSAYYLIAPSEASSNLARYDGVRYGLRVQGHDSVEMMTK
                     TRGEGFGPEVKRRIMLGTYALSAGYYEAYYGQAQKVRTLIIRDYEHAFERFDLLVSPT
                     SPTTAFRLGERTEDPLAMYLSDVFTIPANLAGVPAVSIPCGLDRRGLPIGLQLTAPVL
                     QEARLLAAADAVEQELAFEEVPPLLRDA"
     gene            complement(1557..2444)
                     /locus_tag="VE646_02170"
                     /pseudo
     CDS             complement(1557..2444)
                     /locus_tag="VE646_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            2531..4006
                     /gene="gatB"
                     /locus_tag="VE646_02175"
     CDS             2531..4006
                     /gene="gatB"
                     /locus_tag="VE646_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HZD78833.1"
                     /translation="MTDTLTTRGYEAVIGLECHVELSTRTKMFCGCRNEFGAPPNTNV
                     CPVCLGLPGSLPVPNERAIEYIVRIGLALNCRIAPHSLFHRKNYFYPDMPKDFQISQY
                     DLPICVGGHLDVDVDGRARRIGITRVHMEEDTGKTSHLGGTGRIGRASAALVDYNRAG
                     VPLVEVVSEPDLRSAEEARAYLVELRATLEALGVSDVRMEEGSLRCDANISVRPVGAD
                     TLGTKVEIKNLNSVRSLERALRFEQGRQVDALERGEPLLQETRHFDEDRGSTHTLRSK
                     EEAFDYRYFPEPDLPPLEPIEAWVEEIRSSLPELPAARRQRLIHEHGLKAEQAAIVGG
                     DRGATEYLERTVALGAPAGRVATWMTQDIAAYLNRTGKDWSELSKDFPPEALAELLAR
                     VERGELSSTGSKTAFGIAVGTGRLLEDVIRVEGLRRVSDTSQLEAWVDEAIAENPALV
                     EQFRGGKTPVINALVGQVLRRSPSADPQAVMELLRARLSGS"
     gene            4026..5291
                     /locus_tag="VE646_02180"
     CDS             4026..5291
                     /locus_tag="VE646_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78834.1"
                     /translation="MSTGRGEPKRYPWWQLILLGLVAMVEAVLVRPLRRLWVSDEPLD
                     TYALVHMASAAGDGMVAIALAGSIFFDIPVGEAKVRVALYLLLTMAPLAVAGPILVVL
                     LDRAGPRRAISATAAAGRSLVAVIAAPRVGSLLLFPLAFFLLVLSKVHGITKNGLTMA
                     YAGREEGLVRANARLGRIAVAGVVLAAVPGVLLLKLGSAHAVLYGAAIVYALTTALNL
                     RLPHPRVPRIRGRVGPRGALPALTAPAVGAAGLRAANGFLLFLLAFALRRSGQPAWWY
                     GILGAAATGGLFAADLLAPRLPTSWREEAVVVACVIGAGLGAVVAFAAFSLPVLTLFA
                     LIVGASGELGRLAFQSLMQGAAPGGAHGRVFVRYEVIFQLSWVAGALLPSMLPIEFRE
                     GFLILFGLYLIAGIGYLAPQHLVQRGGPD"
     gene            5370..5681
                     /locus_tag="VE646_02185"
     CDS             5370..5681
                     /locus_tag="VE646_02185"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZD78835.1"
                     /translation="MAEWMTVGEAGDVAEGDVNSYSVGDRSVAVANVDGKLFAFDDVC
                     SHQQCSLSEGDLDDTVIECPCHGSQFDVTTGDVVEGPAADPIDTFEVREGEGQLEVSV
                     E"
     gene            5712..6941
                     /locus_tag="VE646_02190"
     CDS             5712..6941
                     /locus_tag="VE646_02190"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZD78836.1"
                     /translation="MPTHVILGAGLAGGTAAATLRDEGFEGRIVLIGEEPRAPYERPP
                     LSKEYLRGEEADPLFVRPPGWFGEHEVETRFGERARAVDAADRTVVLDSGDRIGFDAL
                     LVATGSRNRRLAVEGADVPGVFQLRTLEDADRIRAAAAGAARAVLVGAGFIGCEAAAS
                     LRSLGLEVAVVEFFATPLQRVLGAELGGVIEGLHRDHGCEMVLGEAVERFEGTDRFEA
                     VITQGGRRVEGDFAVVGVGVEPATEILESSGVLLDGGVLVDAALRTNVPGILAAGDVA
                     RHDHPRFGPIRVEHYDNAIKMGQAAARNMLGRDEIFDDPHWFWSDQYDAQIQMSGFAT
                     GWDRLVYRGDVDERRFAAFMLQEGVLVSAFSMNWPRDVRRAKALIAAGARPDPELLTD
                     PEVDLRRLVPGEAVERA"
     gene            6938..7159
                     /locus_tag="VE646_02195"
     CDS             6938..7159
                     /locus_tag="VE646_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78837.1"
                     /translation="MIGPQITHVPCDLNQQRKRQEARRDSVARAVARRLGRRSLWDRV
                     LRRQPRRPELTAEDLRLPEGEDVHHSLLY"
BASE COUNT         1044 a         2534 c         2683 g         1040 t
ORIGIN      
        1 gagacggtgc tggcgcaggc gccggacccc gccgacgggg cgttccgagt tccgtctccg
       61 caggcgggct gatggcggat ctgctcgagc tgagcgtcca cgaggcgctc gagcggatcg
      121 aggcgcgcga ggtcggccgc gacgagctgc tctcgaccta cgtcgccgcc gcggccgggg
      181 acgagctcaa cgcctacctc tggcgggccg aacccggcaa gcccggctgc gccgggcgcg
      241 gcgaggggcc gctggccggg atcccggttg cggtcaagga cctgttctgc accgagggcg
      301 tcgagaccac cgccggctcg cgcatcctcc agggacaccg gccgctctac acggcgaccg
      361 ccgtgcgaag gctgacggac gccggcgccc gggtgctcgg caagaccaac atggacgagt
      421 tcgcgatggg ctcctccaac gagaactcgg cctacggccc ggtgcgcaat ccctgggacc
      481 tcgggacggt gcccgggggc tcgtccgggg gatcggccgc tgcggtcgcc gcgggagagg
      541 cggtgtgggc gctgggcacc gacaccgggg gctcggtgcg ccagccggcc gcgctgtgcg
      601 gcgtggtggg cctgaagccg acctacggcc tgatcagtcg gtacgggctc atcgcgttcg
      661 cctcctcgct cgataccgtg ggcacgttca cacgatcggt ccgcgacgcc gcggtcctgc
      721 tctcgacgat cgccggcaag gatccgctgg atgccaccag cctggacgag cccaggcgtg
      781 actacgccgg ggatctgggg cagggtgtgg cgggcctccg ggtcggggtg gtgcgcgaga
      841 ccttcgggca gggcgtcgaa cccgacgtgg cgtccgccgt acgctcggcc gtcgaccgct
      901 tcgccgccct cggcgccgag gtcggcgagg cgtcgctgcc gcacgcggac tacgcgctgt
      961 ccgcctacta cctgatcgct ccctcggagg cctcctcgaa cctggcccgg tacgacggcg
     1021 tccggtacgg gctccgggtc cagggccacg actcggtgga gatgatgacg aagacccgag
     1081 gggagggttt cggtccggag gtgaagcgcc ggatcatgct gggaacctac gcgctgtccg
     1141 ccggctacta cgaggcctac tacgggcagg cccagaaggt ccgcacgctg atcatccggg
     1201 actacgagca cgcgttcgag cggttcgacc tgctggtctc acccacctcg cccaccacgg
     1261 cgttccgtct gggggagcgg accgaggatc ctttggcgat gtacctgtcg gacgtcttca
     1321 cgatcccggc caacctggct ggggtcccgg ccgtctccat cccctgcggg ctggaccggc
     1381 ggggactacc gatcggcctc cagctcaccg ccccggtgct ccaggaggcc cgcctcctgg
     1441 ccgccgccga cgccgtcgag caggaactcg ccttcgagga ggtccccccg ctcctgcgtg
     1501 atgcctgagc tcgagacccg ggtggcccgc tcggagcccg ccgagatgat cagggccacc
     1561 gcccccacga tcgtgccgcc cgccacgacc gtccgcaacc cgacggactc gccgaggatc
     1621 agccatccca agaacaccgc gacgatgggg ttcacgtacg cgtaggtcga gaccagcggc
     1681 gtacgagcgt tccggagcag ccagacgtag caggtgaagc ccacccagga gcccaccgcg
     1741 atcaggtaca ggagagcgag caccgagcgg gcggagaagg cggccggatc gacctgggtg
     1801 aactcgcccc gcaccccggc caggacgatc agcgcggccc cccccaccac catctccatg
     1861 ccggcaccga ccaggggtcg agccggcagc gccgccgtgc gcgagtagag cgagccggcc
     1921 gtccagcaga tggtcgcgcc gatcatgacc agcatccccg acagcggagc cgtggtccca
     1981 ctggccccat ccaccagcag cgcggcaccg ccgaacccca ggatcaaccc ggcgaccgtg
     2041 acccatccat gccgatggcg cagcaggatc cggtccacga gcgccatcca gagcgggacc
     2101 agcgccacca tcagcgaggc gatccccgag gggaccgtgc gctcggccag gaccacgccc
     2161 ccgttgcccc ccacgatcaa gcccagcccc accagcaggg cggagcgcca ctgcagcggg
     2221 ccagggcgat cggcgtcccg gtcaccgcgg cggatcgccc acgcgtacag caccccgccg
     2281 gccaccagga accggatccc cgccgccagg agcgagggca gggtctcgtt catgatgcgg
     2341 atcgccaggt aggtcgtgcc ccagaccacg tagacggcgc cgagcgcggc ccagatcagg
     2401 gggatggagg cccgggcatg ctggggctga gccgtccggg acatgggcac caggataggg
     2461 ctccccgcgc tcggtgcccg gcggctaccc tccgggtgcg tgcgccgaac ccgttaagct
     2521 cacggacggc atgacggaca cgctgaccac ccgcggctac gaggccgtca tcgggctgga
     2581 gtgccacgtg gagctctcga cccggaccaa gatgttctgc ggctgccgca acgagttcgg
     2641 ggccccgccg aacaccaacg tctgtcccgt ctgcctgggg ctgccgggct cgctcccggt
     2701 gccgaacgag cgggcgatcg agtacatcgt gcggatcgga ctggccctga actgccggat
     2761 cgccccccac tcgctctttc accggaagaa ctacttctat ccggacatgc cgaaggactt
     2821 ccagatctcc cagtacgacc tcccgatctg cgtgggtggt catctcgacg tcgacgtgga
     2881 tggccgggca cgccggatcg ggatcacccg cgtgcacatg gaggaggaca ccggtaagac
     2941 ctcgcacctg ggggggaccg gtcgcatcgg tcgggccagc gcagcgctgg tcgactacaa
     3001 ccgcgccggg gtcccgctgg tggaggtggt ctccgagccc gacctgcggt cggcggagga
     3061 ggcgcgcgcg tacctggtcg agctccgggc cacgctggag gccctcggcg tctccgacgt
     3121 ccggatggag gaggggtcgc tccgttgcga tgcgaacatc tcggtccgtc cggtgggggc
     3181 cgacacgctc ggcacgaagg tcgagatcaa gaacctcaac tccgtccgct cgctggagcg
     3241 ggcgctccgc ttcgagcagg gccgacaggt cgatgcgttg gagcggggcg agccgctgct
     3301 gcaggagacg cgccacttcg acgaggacag gggcagcacg cacaccctgc ggtcgaagga
     3361 ggaggccttc gactaccggt acttccccga gccggatctg ccgccgctcg agccgatcga
     3421 ggcctgggtg gaggagatcc gcagctcact gcccgagctc ccggcggccc gtcggcagcg
     3481 cctgatccac gagcatggcc tcaaggccga gcaggccgcc atcgtgggag gggaccgagg
     3541 agccacggag tacctcgagc ggacggtggc cctcggtgcg ccggcggggc gggtggccac
     3601 gtggatgacg caggacatcg ccgcctacct gaaccggacc gggaaggact ggagcgagct
     3661 ctcgaaggac ttccctccgg aggcgctcgc cgagctgctg gcgcgggtgg agcggggcga
     3721 gctctcgtcc actggctcca agacggcctt cgggatcgcc gtggggaccg ggaggctcct
     3781 ggaggacgtg attcgggtgg aggggctgcg gcgggtctcg gacacctccc agctcgaggc
     3841 gtgggtggac gaggcgatcg ccgagaaccc cgcgctcgtc gagcagttcc gcggcgggaa
     3901 gacacccgtg atcaacgccc tggtcggcca ggtcctgagg cgatccccct ccgcggaccc
     3961 ccaggccgtg atggagctcc tgcgggcccg tctctcaggg tcctgacgcc cgggggagcg
     4021 gccccgtgag caccggtcgc ggggagccca agcggtaccc ctggtggcag ctgatcctgc
     4081 tcgggctggt ggccatggtc gaggcggtgc tggtccgtcc gctgcggcgc ctgtgggtct
     4141 cagacgagcc gctcgatacc tacgcgctcg tccacatggc cagcgccgcc ggggacggga
     4201 tggtggccat cgccctggcc ggctcgatct tcttcgacat cccggtgggc gaggccaagg
     4261 tgcgggtcgc cctgtacctc ctgttgacga tggccccgct ggcggtggcg gggccgatcc
     4321 tggtggtcct gctggatcga gccggtcccc ggcgagccat ctccgccacg gccgctgccg
     4381 gtcgttcgct ggtcgccgtg atcgccgccc cgagggtggg ctccctcctg ctgttcccct
     4441 tggccttctt cctcctcgtg ctgtcgaagg tccacggcat cacgaagaac gggctgacga
     4501 tggcctacgc ggggcgagag gaggggctgg tccgggcgaa cgcgcgcctg ggccggatcg
     4561 cggtcgccgg cgtggtcctg gcggccgtcc cgggtgtcct gctcctgaaa ctgggcagcg
     4621 cgcatgcggt cttgtacggg gccgccatcg tgtacgcgct caccaccgcg ctcaacctcc
     4681 gcctgccaca cccgagggtc ccgcggatcc ggggccgggt ggggccgcgc ggggcgctgc
     4741 ccgctctgac ggcgccggcg gtcggagcgg cggggctccg agcggcgaac gggttcctgc
     4801 tcttcctgct ggccttcgcg ctccggagga gcggtcaacc ggcctggtgg tacgggatcc
     4861 tcggcgccgc ggcgacgggg ggcctgttcg cggcagacct cctggcgccg cgcctaccca
     4921 cgtcgtggcg ggaggaagcc gtcgtggtgg cctgtgtcat cggggccggt ctgggagcgg
     4981 tggtggcctt cgccgcgttc tccctgccgg tgctgacgct cttcgcgctc atcgtgggag
     5041 cctccgggga gctcggtcgg ctggccttcc agagcctgat gcaaggagcg gcaccgggcg
     5101 gtgcccacgg gcgggtgttc gtacgctacg aggtgatctt ccagctctcc tgggtggcgg
     5161 gggcactgct gccctcgatg ctgccgatcg agttccggga gggattcttg atcctgttcg
     5221 ggctctacct catcgcgggg atcggatacc tcgcccccca gcacctggtg caacggggcg
     5281 gccccgactg atcgcgcgca gacaccggca gcgatgccgg gtgtcctatg ctgggccggt
     5341 ctgtccgagt cggacaagga ggaagcggga tggcggagtg gatgacggtc ggcgaagccg
     5401 gagacgtggc cgagggcgat gtcaactcct actcggtggg ggaccggtcc gtggcggtgg
     5461 ccaacgtgga cggcaagctc ttcgcgttcg acgacgtctg cagccatcag cagtgctcgc
     5521 tgtccgaggg cgacctggac gacacggtga tcgagtgccc ctgccacggg agccagttcg
     5581 acgtcaccac gggcgacgtg gtcgagggtc cggccgcgga ccccatcgac accttcgagg
     5641 tccgggaggg agagggccag ctcgaggtct cggtggaata gcgggcaccc ggccgccgtt
     5701 gcagcgggac gatgcctacc cacgtcatcc tcggggcagg cctggccggt ggcacggccg
     5761 cggccaccct gcgcgacgag gggttcgagg gccggatcgt gctgatcggt gaggagcctc
     5821 gagccccgta cgaacgtccc ccactgtcca aggagtacct gcggggcgag gaggcggacc
     5881 ccctcttcgt tcgcccaccc gggtggttcg gggagcatga agtggaaacg cggttcggcg
     5941 agcgagcccg ggccgtggac gcggccgatc gcaccgtcgt gctcgactcg ggggatcgga
     6001 tcggcttcga cgccctcctc gtcgccaccg gcagccggaa ccggagactg gcggtggagg
     6061 gagcggatgt gcccggcgtc ttccagctcc ggaccctgga ggatgccgat cggatccgcg
     6121 cggcggccgc cggcgccgcc cgcgccgtgc tggtgggcgc ggggttcatc ggatgcgagg
     6181 cggcggcctc gctccgctcc ctgggcctcg aggtggcggt ggtcgaattc ttcgccactc
     6241 ctttgcagcg cgtgctgggg gccgagctcg gcggggtgat cgaagggctc caccgcgatc
     6301 acggctgcga gatggttctc ggcgaggccg tcgagcgctt cgagggcacc gaccggttcg
     6361 aggccgtgat cacgcagggt ggccggcggg tcgagggcga cttcgcggtg gtcggcgtgg
     6421 gcgtcgagcc ggcgaccgag atcctcgaga gctccggcgt cctgctggac ggcggcgtcc
     6481 tggtggacgc ggcgctccgc acgaacgtgc ccgggatcct cgccgcgggg gacgtggcgc
     6541 ggcacgatca tccccggttc gggcccatcc gcgtggagca ctacgacaac gccatcaaga
     6601 tgggacaggc ggcggcccgg aacatgctgg gacgggacga gatcttcgac gatccgcact
     6661 ggttctggtc cgatcagtac gacgcccaga tccagatgtc cgggttcgcc accggatggg
     6721 accggttggt ttaccgtgga gacgtggacg agcgacggtt cgcggccttc atgctgcagg
     6781 agggggtcct ggtgtccgcg ttcagcatga actggcctcg cgatgtccgc cgggccaaag
     6841 cgctgatcgc ggcgggcgcc cgaccggatc ccgagctgct gacggacccc gaggtggatc
     6901 tgcggcggct ggtccccggc gaggcggtgg aacgggcatg atcggacccc agatcaccca
     6961 tgtcccatgc gacctgaacc agcagcgcaa gcggcaggag gcccgccggg actcggtggc
     7021 acgtgccgtg gcccgacggc tgggcagacg gtcactgtgg gaccgggtgc tgcgacggca
     7081 accgcggcgc cccgagctca ccgccgaaga cctgcgcctg ccggagggcg aggacgtcca
     7141 ccacagcctg ctctactgat aggctgcgcg ccacggcgcg gccccggcgc cgaccgatca
     7201 ccgaccgaga tcccatgagg agtgaacgtc ccgtggacag gcccgagatc atcctggctc
     7261 ccggcccgac gcccatcccg cacgaggtgc tgctcgctca g
//
LOCUS       DASQQQ010000046         3564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_251881, whole genome shotgun sequence.
ACCESSION   DASQQQ010000046 DASQQQ010000000
VERSION     DASQQQ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251881"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1611
                     /locus_tag="VE646_02200"
     CDS             <1..1611
                     /locus_tag="VE646_02200"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HZD78838.1"
                     /translation="IEYSHRFLGVVVGVLILLTAWYAWRRRRGDALVVWPALLAVPLV
                     LVQGAIGAGRIFVGPNPWIVTVHLLVAMAVVATVMVTAASATVPEGGGEPEHPGIGPL
                     AWTTLWATAAVLLIGAYVRGAHASLVFLDWPLMDGTIVPRLGGEAALVTFLHRGLAAV
                     AVVLGGMLAFRSRRSPHRSIRLLGWAAFGLLLLQAGIGGAVVLTRLAPVAVAGHVAGG
                     SLAWAALVALLTVDGRLRRRAEGGERPTARQVAGAYAQLMKPDIIVLLLITTVPTMIL
                     AAGGLPPAGLIAATLVGGTMAAGGANALNHYFDRDIDEVMARTRSRPIPAHAIAPDRA
                     AWFGASLSGLSFVWLATTVNLLAATLALSAIAFYVGVYTLWMKRSTPQNIVIGGAAGA
                     VPVLVGWAAVTGTVGLPAWLLFAVVFFWTPPHFWALSMKYAGDYAEAGVPMLVVVRGG
                     RETAVQIVLYAIQTVAVSLLLAPAAGMGPTYLFAAGALGAAFVWYAAGVLRGGTTRAA
                     MHLFTFSITYLALLFGAVALDTLAGAAA"
     gene            1608..1823
                     /locus_tag="VE646_02205"
     CDS             1608..1823
                     /locus_tag="VE646_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78839.1"
                     /translation="MTAFLVELIRNHGWHAIGELAALALITVFTVLYATALVRGRVRA
                     VRCPSCGRLASRARPTCPRCGTTLETT"
     gene            1903..3018
                     /locus_tag="VE646_02210"
     CDS             1903..3018
                     /locus_tag="VE646_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HZD78840.1"
                     /translation="MGFDRARRTKASRRRLRLAAFIAVLAAALLAACSRGLPQSSLEP
                     AGPRAERIDTLFQVVFWIAAGVFVLVEGAIVYVSIRYRRRKGREDAPVQIHGNTRLEI
                     IWTVVPALILAGLAFPTVSTIFDLSRQPRGENVLHVDVIGHQWWWEFQYPDLDIVTAN
                     ELHIPVGTEVALTLQSKETPGGLAGFNDQGKPIPQNAIPVIHSFWVPRLSGKQDLVPG
                     HVEHLSMAADEPGTYRGQCAEFCGTSHANMRLRVIAQTQADFEGWTATMQQPPAMPEP
                     GSPAAKGAELFQNFGNGSCLACHSVDPALGGTVAPNLRGLAGRQTFAAGLFEVNEKNL
                     RRWLENPRAMKPGVVMPDYGLDQEQIEAIVAYLLTLK"
     gene            3038..>3564
                     /locus_tag="VE646_02215"
     CDS             3038..>3564
                     /locus_tag="VE646_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZD78841.1"
                     /translation="MAIAQPLPRTSIFQRPTATTGLWSWITTVDHKRIGLLYGVTAFG
                     FFLVGGIEALLIRWQLATPNGTLLDAESYNQLFTMHGLTMIFLVVMPLSAAFFNYLVP
                     LMIGARDVAFPRLNAFSYWVFLFGGLFIYSSWFLGGAPDGGWFGYAPLSSQSPGINMD
                     YYAVGLQIVGLGSLLS"
BASE COUNT          424 a         1337 c         1222 g          581 t
ORIGIN      
        1 atcgagtact cgcaccgctt cctgggcgtg gtggtcggcg tcctgatcct gctgacggcc
       61 tggtacgcct ggcgccgcag gcggggggac gccctcgtcg tctggccggc cctgctcgcc
      121 gtccccctgg tcctcgtgca gggcgcgatc ggggcgggga ggatcttcgt cggccccaac
      181 ccctggatcg tcacagtcca cctgctcgtc gccatggccg tcgtcgcgac cgtcatggtc
      241 acggccgcct cggcgacggt gcccgaggga ggcggcgagc ccgagcatcc cgggatcggc
      301 ccgctcgcct ggaccacgct ctgggcgacg gcggccgtcc tgctgatcgg cgcctacgtc
      361 cggggcgccc acgcgagcct cgtcttcctc gactggcccc tgatggacgg gacgatcgtc
      421 ccccgactgg ggggcgaggc cgccctcgtg acgttcctgc accggggcct ggcggccgtc
      481 gccgtcgtgc tcggcggcat gctggcgttc cggtcccggc gctcccccca ccggtcgatc
      541 cgtctcctcg gctgggccgc cttcggcctg ctgctgctcc aggcggggat cgggggcgcc
      601 gtggtgctga cgcgcctcgc cccggtggcc gtggccggcc acgtcgcggg cggcagcctc
      661 gcatgggccg cgctcgtggc cctcctgacc gtggatggcc gactccggcg ccgggcggag
      721 ggcggcgagc ggccgacggc ccgtcaggtg gccggggcct acgcgcagct gatgaagccc
      781 gacatcatcg tcctcctgct catcacgacg gtccccacga tgatcctggc ggcgggcggg
      841 ctgccccccg ccggcctcat cgcggccacg ctcgtcggcg gcacgatggc ggccggcggc
      901 gccaacgccc tcaaccacta cttcgaccgg gatatcgacg aggtcatggc caggacccgt
      961 tcgcgtccga tcccggccca cgcgatcgcg cccgaccggg ccgcgtggtt cggcgcctcc
     1021 ctgtcgggcc tgtcgttcgt gtggctggcc accaccgtca acctgctggc cgcgaccctc
     1081 gccctctcgg cgatcgcctt ctacgtgggc gtctacaccc tgtggatgaa gcgctccacc
     1141 ccgcagaaca tcgtgatcgg tggcgcggcc ggggccgttc cggtcctggt cggctgggcg
     1201 gcggtcaccg gcaccgtcgg gctcccggcc tggctgctgt tcgccgtcgt gttcttctgg
     1261 acccccccgc acttctgggc cctgtcgatg aagtacgcgg gcgactacgc ggaggcgggg
     1321 gtgccgatgc tggtcgtcgt ccggggcggc cgggagacgg ccgtccagat cgtgctgtac
     1381 gcgatccaga ccgtggccgt ctcgctgctc ctcgcccccg cggcggggat ggggccgacc
     1441 tacctgttcg ctgccggcgc gctcggggcg gcgttcgtct ggtacgcggc cggggtgctg
     1501 cgcggcggca cgacccgcgc ggcgatgcac ctgttcacct tctcgatcac ctacctcgcg
     1561 ctgctgttcg gcgccgtggc cctggacacg ctggcggggg cggccgcatg accgcgttcc
     1621 tcgtcgagct gatacgcaac catggctggc acgcgatcgg cgagctcgcc gccctggctc
     1681 tgatcaccgt gttcaccgtc ctgtacgcga ccgcgctcgt ccggggacgg gtgcgtgcag
     1741 tccggtgccc gtcgtgcgga cggctggcct cgcgggcccg cccgacgtgc ccccggtgcg
     1801 ggaccaccct ggagacgacc tgagcgcgtt tcggggaccg gggggggccg tgatatcgtt
     1861 ctgcgctgcg gccggggggc gacccgaccg cccacggatc tgatgggttt cgatcgcgcg
     1921 cgcagaacga aggcatcccg caggcggctc cggctggccg cgttcatcgc cgtcctcgcc
     1981 gccgccctcc tcgccgcctg ctcccggggc ctcccccaga gcagcctgga gcccgccggt
     2041 cctcgcgccg aacggatcga caccctcttc caggtcgtct tctggatcgc cgccggcgtg
     2101 ttcgtgctgg tggaaggggc gatcgtctac gtgagcatcc ggtaccggcg ccgcaagggc
     2161 cgcgaggacg cccccgtgca gatccacggc aacacccggc tcgagatcat ctggaccgtg
     2221 gtccccgccc tgatcctggc cggcctggcc ttcccgaccg tgtccacgat cttcgacctg
     2281 tcccggcagc ccaggggcga gaacgtgctc cacgtcgacg tgatcggcca ccagtggtgg
     2341 tgggagttcc agtaccccga cctcgacatc gtgacggcga acgagctcca catccccgtc
     2401 ggcacggagg tcgcgctcac cctccagtcc aaggagacgc cgggcgggct cgccggcttc
     2461 aacgaccagg gcaagccgat cccccagaac gcgatcccgg tgatccacag cttctgggtg
     2521 ccccgcctca gcgggaagca ggatctcgtc cccggccacg tcgagcacct gtcgatggcg
     2581 gccgacgagc cggggaccta ccgggggcag tgcgcggagt tctgcggcac ctcccacgcg
     2641 aacatgcgcc tgcgggtgat cgcccagacg caggccgact tcgagggctg gaccgcgacc
     2701 atgcagcagc cgcccgcgat gcccgagccg gggagcccgg ccgcgaaggg ggccgagctc
     2761 ttccagaact tcggcaacgg gtcctgcctc gcgtgccact cggtcgaccc cgcgctgggc
     2821 gggaccgtgg ccccgaacct ccggggcctg gccggccgcc agaccttcgc cgcgggcctg
     2881 ttcgaggtca acgagaagaa cctgcgccgg tggctggaga acccccgcgc gatgaagccc
     2941 ggggtcgtga tgcccgacta cggactggac caggagcaga tcgaggcgat cgtcgcctac
     3001 ctgctcacgc tgaaatgatg ggctgggtga tgcactgatg gcgatcgcgc aaccgctccc
     3061 ccgcacctcg atcttccagc ggccgacggc caccaccggc ctctggtcct ggatcacgac
     3121 ggtcgaccac aagcggatcg gcctcctcta cggcgtgacc gccttcgggt tcttcctcgt
     3181 gggggggatc gaggccctgc tgatccgctg gcagctcgcc acgccgaacg gaaccttgct
     3241 cgatgccgag agctacaacc agctcttcac gatgcacggg ctcacgatga tcttcctcgt
     3301 cgtgatgccg ctgtccgccg ccttcttcaa ctacctcgtc ccgctgatga tcggcgcccg
     3361 cgacgtcgcc ttcccccggc tgaacgcgtt cagctactgg gtgttcctgt tcggcggtct
     3421 gttcatctac tcctcgtggt tcctcggcgg cgcccccgac ggcgggtggt tcggctacgc
     3481 gccgctctcg agccagtcgc ccggcatcaa catggactac tacgcggtgg gcctgcagat
     3541 cgtcggtctc gggtccctgc tgtc
//
LOCUS       DASQQQ010000047         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_113934, whole genome shotgun sequence.
ACCESSION   DASQQQ010000047 DASQQQ010000000
VERSION     DASQQQ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113934"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(317..1921)
                     /locus_tag="VE646_02220"
     CDS             complement(317..1921)
                     /locus_tag="VE646_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZD78842.1"
                     /translation="MQAYRRPPADLLHTGFMREARRHWGRLALADTTGRELSFGRALT
                     ASLALASVLARRTPGERTVGVLLPASVGGALANLALLSAGRVPVNLNFTIGPDAMEAA
                     VAESGIRTIVTSRRFLERANVAGTPGMIFLEELGSAIGAGRRLLAFLEARLAPLGLLR
                     RRYGGRGVTPDSLATVIFSSGSSGEPKGVMISHANVLANVDALGDVFSLYPEDRFIGV
                     LPFFHAFGFTGTFWFPLLQGAGVVYHPNPMDAKTVGELAERYRATMVISTPTFCAAYL
                     RRVTPEQFAHLRYVVVGAEKLRAPLACAFREKYGVWPLEGYGCTEMSPVVAINRPDVV
                     LDGMTVEVGTKAGSVGRPIPGVRAKVVDLETGEGPLVGREGLLLVRGPNLMRGYLKQP
                     ERTAEAIRDGWYVTGDIATIDEDGFITITDRLSRFSKIGGEMVPHLKIEEAIDAVLGE
                     GRSAVTAVPDAARGERLVAFYTGTDLTADELWERLRRTDLPRLWLPRPDCLFALEGIP
                     TLGTGKVDLRALRVLAAERVRVADGG"
BASE COUNT          322 a          756 c          723 g          291 t
ORIGIN      
        1 atgatggccc aggtccgtgg gcccgacgcc accgttcccc tcggaagccg tacgtcgagc
       61 tcgaacccct cgaactcgta gatcatcgag agcccctcgg gggctcacgc catctccagc
      121 tcgtcttcac gtcctcttca cgcacccttc aagcgaggat ctcatccggg agcacgcccg
      181 gaggagcggg ttccggccga caagatcacc gaggtccaca ccatgttcga ccccgccacg
      241 gggcactgct gatggacccg cgggggccgc gccggccggt tcggcggcgc cgtccgcccc
      301 tcgcgggcgc ctcgaatcat cctccatccg ccaccctcac ccgctcggcc gccagcaccc
      361 gcagggcccg caggtccacc ttgcccgtgc cgagggtggg gatgccctcc agcgcgaaca
      421 ggcaatccgg tctgggcagc cagagccggg gcaggtcggt gcggcggagc cgttcccaca
      481 gctcgtcggc cgtgaggtcc gtccccgtgt agaacgctac caggcgctcg ccccgggccg
      541 cgtcgggcac cgcggtcacc gcggagcgcc cttcgcccag cacggcgtcg atggcctcct
      601 cgatcttcag gtgcggcacc atctcgccgc cgatcttgct gaagcgagac agccggtcgg
      661 tgatggtgat gaagccgtcc tcgtcgatcg tggcgatgtc ccccgtcacg taccagccgt
      721 cacggatcgc ctcggccgtg cgctccggct gcttcaggta gccccgcatg aggttggggc
      781 ctcggacgag caggagcccc tcgcggccga cgaggggacc ctcacctgtc tccagatcca
      841 ccaccttcgc cctcacgccg gggatgggtc tgccgaccga tccggctttc gtaccgacct
      901 ccaccgtcat tccgtcgagg acgacgtcgg gccggttgat ggccaccacc ggcgacatct
      961 cggtacagcc gtagccttcc agaggccaca ccccgtactt ctcgcggaac gcgcaggcca
     1021 ggggcgcgcg cagcttctca gcacccacca caacgtagcg gaggtgggcg aactgctccg
     1081 gcgtcacccg gcgtaagtag gccgcgcaga aggtcggcgt gctgatcacc atggtcgccc
     1141 ggtaccgctc ggccagctcg cccacggtct tcgcgtccat ggggttggga tggtacacga
     1201 cgccggcgcc ctggaggagg gggaaccaga acgtgcccgt gaagccgaag gcgtggaaga
     1261 agggcagcac gccgatgaac cgatcctccg ggtagaggga gaacacgtcc cccagggcgt
     1321 ccacgttggc cagcacgttg gcgtgggaga tcatcacccc cttcggctcg ccgctgctgc
     1381 cgctggagaa gatcacggtg gccagggagt cgggggtcac gccacgtccg ccgtaccgcc
     1441 gccggaggag gccgagggga gcgaggcgcg cctccagaaa cgcgaggagc cgccgcccgg
     1501 caccgatcgc cgatcccagc tcctccagga agatcatgcc gggggtcccg gcgacgttcg
     1561 ccctttcgag gaagcgtctc gaggtgacga tcgtgcggat gccgctctcg gccaccgcgg
     1621 cctccatggc gtcgggcccg atggtgaagt tcaggttcac cggaacgcgg ccggccgaca
     1681 gcagggcgag gttggcgagg gcgcccccca ccgaagccgg cagcaggacg ccgacggtgc
     1741 gctctccagg ggtccgccga gcgagaacgg aggccagggc cagggaggcg gtcagagccc
     1801 tgccgaagct gagctcccgg cccgtggtgt cggccagggc gaggcggccc cagtgccgcc
     1861 gggcctcgcg catgaagccg gtgtgcagca ggtccgctgg cggccgtcga tacgcctgca
     1921 cggcccacct ccccgtcggg gatcggaagg aaggggccgc ccatccggtg gtgccttcgg
     1981 gcggggtgcg aaccccgagg gcggtccggg ggtcggtcgg tcttcaggat cggcgccgca
     2041 atccatccgt gtcaaggatc tccaccgccg tggaggggca atgcgcggag ac
//
LOCUS       DASQQQ010000048         5766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_31223, whole genome shotgun sequence.
ACCESSION   DASQQQ010000048 DASQQQ010000000
VERSION     DASQQQ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31223"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VE646_02225"
     CDS             complement(<1..723)
                     /locus_tag="VE646_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HZD78843.1"
                     /translation="MKAALCTRYGPPEVIRLAEVPTPTRVDREVLVRVHATTVNRTDC
                     GIRAGHPWFARSLTGVVRPKVPILGGEFAGVIQAVGSGVTRFGVGDRVFGYAADGFGA
                     HAEQLTIAEDGALATMPQGRTFEEVAPSTEGAHYAMTNIEGAGVRPGQRVLVNGGTGG
                     IGSAAVQILKSMGAHVTAVCRGEHAELVRGLGAERIIDFTTEDFRKDDQRYDVVFDAV
                     GKSSFGACRGLLEPHGLYLSTDL"
     gene            complement(794..3253)
                     /locus_tag="VE646_02230"
     CDS             complement(794..3253)
                     /locus_tag="VE646_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZD78844.1"
                     /translation="MEETDPEAVDDRPLADALARPLADALAHPLDGPAAGPVPNPLDG
                     PVPTFEAREAEQVAASAFGVRGTASPLESERDQNFRIEVPDGPSWVLKISNAVEDPAV
                     VDMQTRAMLHIARTAPGLPVMRIAPTLEGHFHASIEDDDGRPHLVRLVTFMPGRKVEA
                     GDLTLEAVRGFGVCAAELGRALRGFFHPAAGHPLLWNLRHAMRLRELIGNIEDQRRRG
                     LVERVLDRYQDHVAPVFEQLRAQVIHNDLTLDNALLDQDQRITGVLDFGDMGHSALVC
                     DLVATAEPLVGERPDHLEALAAVAAGFTSVTPLTDEELDLLPELLLARWTTSVVISAW
                     RVRAHPDNAEYISGWDQGAWTMLGIFDELGIEGWRARVRGAVLSATGGRARPPSTTEQ
                     LIERRSRLLGSAISPLSYERPIHMVEARGAWMTDAEGRRYLDAYNNVPVVGHSHPRVV
                     AAIARQARTLNTNTRYLHRSALELAERLIGTMPDGLDTVLLVNSGSEANDLAWRLATT
                     FTGGRGGLVSEYAYHGVSTAIAELSPETWREGSIPPHVARIPAPDGYRGPIRREEEGW
                     ELGYAGFVDRALDELAGRGIAPAAMFVDPGFTSDGIFAPPPAYLQEAVRRWRDAGGLF
                     VADEVQTGFGRPGSHLWGFQAHGLVPDVVTLGKPMGNGHPVAAVITRADIVDRFARST
                     EWFSTFGGNPVACEAALAVLDVLDEEELLPHAAQIGQHLRAGLTRLMDRHAWIGDVRS
                     MGLLIGVELVRDRAERDPAAEETEAVLNGMREAGVLIGSTGRGENVLKIRPPLVISRD
                     EADLLLDRLDAVLGDPEAPRT"
     gene            3305..5731
                     /locus_tag="VE646_02235"
     CDS             3305..5731
                     /locus_tag="VE646_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZD78845.1"
                     /translation="MKDRARVVIVGGGVGGCSLAYHLAKLGLTDVVLLEKGELTSGST
                     WHAAGLCTQFNASLHLTKLLMDSLELYRSLEAETGQAVGLHEVGSIRLGTRGERIDEF
                     RRAQGRARVAGLPFELIGPERIRELCPLLSLDDVLGAAYLPTDGHVDPAGVTNAMARG
                     AAALGVAIERRTRVTGLSPTAEGGWQVHTERGAIACEIVVNAAGMWAPEVGRLAGVSL
                     PIVPLQHEFIVTDRIEALDDLPGEIPVVRDSDRSFYVRAEGEGLIVGPFEPQPRAWAV
                     QGIPEGFEQRLLEPDLDRLEGVLELATDRIPALAEVGIMRVVNGPDGYTPDGGCLMGW
                     VPGVRNLFVLAGFSIFGIVFGGGAGRYAAEWIVDGQPSEDMWDVDVRRFGPYAAAAPF
                     LVARALDVYAREYAIHFPHEEHAAGRPVKTDPLYERLGERGAVFGFRFGWERPLWFAP
                     PGTEPREEPTFRRPNWFEHVGRECRAVREGVGVLDQTSFGTYEVRGPGASALLDHLCA
                     NVLPGRDGRIVVTQMLTERGGIECDLTVTRLGPDRYYVVSAAATEIHDLAWIERHLPG
                     DGTVTLENVTDRTGVLTVAGPRSRELLQVLTSEDLTNEAFPYMTARTIRFGSVPVLAM
                     RLSYVGELGWELHHPIEHLAELYDRLVEAGRDLGLVDFGYRALDSLRMEKGYRLWGVD
                     ISPDRTPLEAGLERFVRLDKGDFIGRQALLRQREEGLRQTLACLTIDVEDAIPHGAEP
                     ILADDALVGHVAAAEHGHVVGATIALGYLPVDAARPGTELSVQILGRCRAATVVTAPL
                     FDPENARLHS"
BASE COUNT          756 a         2107 c         2088 g          815 t
ORIGIN      
        1 gaggtcggtc gacaggtaca ggccgtgcgg ctcgagcagg ccacggcacg ctccgaacga
       61 gctcttgccg accgcgtcga agaccacgtc gtaccgctga tcgtccttcc ggaagtcctc
      121 ggtcgtgaag tcgatgatcc gctcggcgcc cagtccgcgc accagctccg cgtgctcgcc
      181 gcggcacacg gcggtcacgt gcgcccccat gctcttcagg atttggaccg ccgccgatcc
      241 gatcccgccg gtcccgccgt tgaccagcac gcgctgcccc ggccggaccc cggccccctc
      301 gatgttcgtc atggcgtagt gggcgccctc ggtgctgggg gcgacctcct cgaacgtcct
      361 gccctggggc atggtggcga gcgccccgtc ctcggcgatc gtcagttgct cggcgtgcgc
      421 gccgaacccg tccgccgcgt acccgaacac ccggtcgccg accccgaacc gggtgacgcc
      481 gctgcccacg gcctggatca ccccggcgaa ctcaccgccc aggatcggca ccttcggccg
      541 gacgaccccg gtgagggacc gggcgaacca cgggtggccg gcccggatgc cgcagtcggt
      601 ccggttcacc gtcgtcgcgt gcacccggac cagcacctcc cgatcgaccc gcgtgggggt
      661 gggcacctcg gcgagccgga tcacctccgg cgggccgtac cgggtgcaga gggcggcctt
      721 catgtcccca accctagccg tcagctcgtc tgcctcccga cccggggccg acggagaggg
      781 cggcacgagg tccttacgtg cggggcgcct ccggatcccc caggacggcg tccagccgat
      841 ccagcagcag atcggcttca tcgcgtgaga tcaccagcgg gggtcggatc ttcagcacgt
      901 tctcaccgcg gccggtcgag ccgatcagga cgcccgcctc gcgcatcccg ttcaggaccg
      961 cctcggtctc ctcggctgcc ggatcccgct ccgcacgatc gcggaccagc tccacgccga
     1021 tcagcagccc catcgagcgg acgtccccga tccacgcatg ccgatccatc agcctggtca
     1081 gccccgcgcg caggtgctgg ccgatctggg ccgcgtgggg gagcagctcc tcctcgtcga
     1141 gcacatcgag caccgcgagc gccgcctcac aggccaccgg gttgcccccg aacgtgctga
     1201 accactcggt cgagcgggcg aaccggtcca cgatgtccgc cctggtgatc accgccgcca
     1261 ccgggtggcc gttgcccatc ggcttgccca aggtgacgac gtccggcacc aggccgtggg
     1321 cctggaaccc ccacaggtgc gaccccgggc ggccgaagcc cgtctggacc tcgtcggcca
     1381 cgaacagccc gccggcgtcc cgccaccggc gcaccgcctc ctgcaggtag gcgggcgggg
     1441 gcgcaaagat gccgtcgctg gtgaacccgg ggtccacgaa catcgccgcc ggggcgatgc
     1501 cgcgcccggc cagctcgtcc agggcccggt ccacgaaccc cgcgtacccg agctcccagc
     1561 cctcttcctc gcggcggatc ggaccccggt agccgtcggg agccgggatc ctggccacgt
     1621 gcggcggtat cgagccctcc cgccaggtct ccggcgacag ctcggcgatc gcggtggaga
     1681 cgccgtggta ggcgtactcg ctcaccaggc caccccggcc gccggtgaag gtggtcgcca
     1741 ggcgccaggc cagatcgttc gcctcgctcc ccgagttcac gagcagcacg gtgtccaggc
     1801 cgtccggcat cgtgccgatc agccgctcgg cgagctcgag cgccgagcgg tgcaggtagc
     1861 gggtgttcgt gttcagcgtg cgggcctgcc gggcgatcgc cgccaccacc cgaggatggc
     1921 tatgtcccac gacggggacg ttgttgtagg cgtccaggta ccggcggccc tcggcatcgg
     1981 tcatccaggc gccccgggcc tccaccatgt ggatcgggcg ctcgtacgac agcggcgaga
     2041 tcgccgagcc gagcagcctg ctccggcgct cgatcagctg ctccgtcgtg gaggggggcc
     2101 gggccctgcc gccggtggcg gacagcacgg cgccccgcac ccgggcccgc cagccctcga
     2161 tccccagctc gtcgaagatc ccgagcatcg tccaggcgcc ctggtcccag cccgagatgt
     2221 actcggcgtt gtcggggtgg gcccggaccc gccaggccga gatcaccacg ctcgtggtcc
     2281 accgcgccag cagcagctcg ggtagcaggt cgagctcctc gtcggtcagc ggcgtgaccg
     2341 aggtgaaccc ggccgcgacc gcggccaggg cctcgaggtg gtcgggccgc tcgcccacga
     2401 gcggttcggc cgtggccacc aggtcgcaga ccagggcact gtggcccatg tcgccgaagt
     2461 ccagcacccc ggtgattcgc tgatcctggt ccagcagggc gttgtccagc gtgaggtcgt
     2521 tgtggatcac ctgggcccgg agctgctcga agacgggggc gacgtggtcc tggtaccggt
     2581 ccaggacccg ttccaccagg cctcgccggc gctggtcctc gatgttgccg atcagctcgc
     2641 gcagtcgcat cgcgtgccgc aggttccaca gcagcgggtg cccggccgcg gggtggaaga
     2701 acccccggag ggctcggccg agctcggccg cgcacacgcc gaagccgcgc acggcctcca
     2761 gggtcaggtc gcccgcctcc accttccgcc cgggcatgaa cgtgaccagc cggaccaggt
     2821 gcgggcggcc gtcgtcatct tcgatcgagg cgtggaagtg cccctcgagg gtgggggcga
     2881 tccgcatcac gggcagtccg ggagcggtgc gggcgatgtg gagcatggcc cgggtctgca
     2941 tgtcgaccac ggccggatcc tccaccgcgt tgctgatctt cagcacccag ctcgggccgt
     3001 cggggacctc gatccggaag ttctggtcgc gctcgctctc caggggcgag gccgtccccc
     3061 ggacgccgaa ggcggatgcc gcgacctgtt cggcctcgcg cgcctcgaac gtggggaccg
     3121 ggccgtcgag ggggttgggg accgggccgg cggccgggcc gtcgaggggg tgggcgaggg
     3181 cgtctgcgag gggccgggcg agggcgtctg cgagggggcg gtcgtccacc gcttcggggt
     3241 ccgtctcctc caccgatcgc tcctttggcc cgggctcggg gcgcccagtg taggctcccg
     3301 ctcggtgaag gaccgcgccc gcgtcgtgat cgtcggtggt ggggtgggtg gatgcagcct
     3361 cgcctaccac ctggccaagc tcggtctcac cgacgtggtc ctcctcgaga agggcgagct
     3421 gaccagcggg tccacctggc acgcggcggg gctctgcacg cagttcaacg cgagcctgca
     3481 cctcaccaag ctcctgatgg acagcctgga gctgtaccgg tcgctggagg ccgagactgg
     3541 gcaggcggtc ggcctgcacg aagtggggag catccgcctg ggcaccaggg gcgagcggat
     3601 cgacgagttc cgccgggcgc agggccgggc ccgggtggcc ggtctgccct tcgagctgat
     3661 cggccccgag cggatccgcg agctctgccc cctgctctcg ctggacgacg tcctgggcgc
     3721 cgcgtacctg ccgaccgacg ggcacgtcga ccccgcgggc gtgacgaacg cgatggcccg
     3781 gggcgcggcc gcgctggggg tggcgatcga gcgccgcacc cgcgtcacgg gcctgtcgcc
     3841 caccgccgag ggggggtggc aggtgcacac cgagcggggg gcgatcgcgt gcgagatcgt
     3901 ggtgaacgcc gccggcatgt gggcgcccga ggtgggcagg ctcgccggcg tctccctgcc
     3961 gatcgttccc ctccagcacg agttcatcgt caccgaccgg atcgaggctc tggacgacct
     4021 gcccggcgag atccccgtgg tccgcgattc cgatcgctcg ttctacgtcc gggccgaggg
     4081 cgagggcctg atcgtcggac cgttcgagcc ccagccccgg gcctgggcgg tgcagggcat
     4141 ccccgaaggg ttcgagcagc ggctgctcga gcccgacctc gaccggctcg agggcgtcct
     4201 cgagctggcc accgaccgga tcccggccct ggccgaggtg gggatcatgc gcgtggtcaa
     4261 cggtcccgac ggctacacgc ccgacggcgg ctgcctgatg ggctgggtcc cgggggtgcg
     4321 gaacctgttc gtactggccg gcttcagcat cttcgggatc gtgttcggcg gcggggcggg
     4381 ccgatacgcg gcggagtgga tcgtcgacgg ccagccgagc gaggacatgt gggacgtcga
     4441 cgtgcgccgg ttcggcccgt acgcggcggc ggccccgttc ctggtcgcca gggccctgga
     4501 cgtctacgcg cgcgaatacg cgatccactt cccccacgag gagcacgccg cgggccgccc
     4561 ggtgaagacc gacccgctgt acgagcggct cggtgagcgc ggggcggtct tcggcttccg
     4621 cttcggctgg gagcgaccgc tgtggttcgc cccgcccggc accgagccgc gcgaggagcc
     4681 cacgttccgc cggccgaact ggttcgagca cgtcggccgg gagtgccgcg cggtgcggga
     4741 gggggtcggg gtcctggacc agacgagctt cggcacgtac gaggtgcgcg ggccgggcgc
     4801 gtcggcgttg ctcgatcacc tgtgcgcgaa cgtcctgccc ggccgcgacg ggcgcatcgt
     4861 ggtcacccag atgctgaccg agcgcggtgg gatcgagtgc gacctcacgg tcacgcggct
     4921 cggcccggat cggtactacg tggtgagcgc cgccgcgacc gagatccacg acctggcctg
     4981 gatcgagcgg cacctcccgg gtgatgggac cgtgacgctc gagaacgtga ccgatcgaac
     5041 gggcgtgctg acggtcgcgg gtccccgctc gcgcgagctc ctccaggtgc tcacctccga
     5101 ggacctgacg aacgaggcct tcccgtacat gaccgcccgg accatccggt tcggctcggt
     5161 cccggtgctg gcgatgcgcc tgtcctacgt gggcgagctc ggctgggagc tgcaccaccc
     5221 catcgagcac ctggccgagc tgtacgaccg gctggtcgag gccggccggg atctggggct
     5281 cgtggacttc ggctaccgcg ccctggactc gctccgcatg gagaaggggt accggctctg
     5341 gggcgtcgac atctcgcccg accgcacgcc gctcgaggcc ggcctggagc ggttcgtccg
     5401 gctggacaag ggcgacttca tcggtcggca ggccctgctc cgtcagcgcg aggaggggct
     5461 ccggcagacg ctcgcctgcc tcacgatcga cgtggaggac gccatccccc acggcgccga
     5521 gcccatcctg gcggacgacg ccctcgtcgg tcacgtggcg gccgcggagc acgggcacgt
     5581 cgtcggagcg acgatcgccc tgggatacct gccggtcgac gcggcccggc ccggcaccga
     5641 gctctcggtg cagatcctgg gtcggtgccg agcggccacg gtggtcacgg cgccgctgtt
     5701 cgacccggag aacgccaggc tccactcgta gcggagcagc ctgacgatcg cgcgtcggat
     5761 ctcgaa
//
LOCUS       DASQQQ010000049         6646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_59057, whole genome shotgun sequence.
ACCESSION   DASQQQ010000049 DASQQQ010000000
VERSION     DASQQQ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59057"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VE646_02240"
     CDS             complement(<1..235)
                     /locus_tag="VE646_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018562863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZD78846.1"
                     /translation="MAAIDQGITFEELELATRNQGMPLEALRYDLTPVGMHYQLVHFD
                     VPALDASAWRLTVEGNVERSVELSLPDIRRRPAV"
     gene            352..981
                     /locus_tag="VE646_02245"
     CDS             352..981
                     /locus_tag="VE646_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HZD78847.1"
                     /translation="MELRSDSISEGQAIPHRYALATPTPEGKAELTGEDVSPHLRWSG
                     EPEGVRSFAVSVVDPDVPADASRMEVEGLALGRDEPRVDFAHWLIVDIPPELHEIPEG
                     ADGDRFVAHGKPPGPTGFGGLSGQNGYTGLFAGSEERAGTYGGWDGPYPPWNDEVVHR
                     YVFTVYALDVPSVGLQPGFTLDEFRRAIDGQVLDTGELLATYTLNPRLR"
     gene            1152..1640
                     /locus_tag="VE646_02250"
     CDS             1152..1640
                     /locus_tag="VE646_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HZD78848.1"
                     /translation="MGSTRETGNGVPSMLTEEGKRRLEERAKRLREEVIPDIVARLEM
                     PERDAQVDAEFQRATDELADIDYLIRHAPVAEQVPGDPQVVEVGDEVEVRFDDGSTDH
                     FLIVHPVEATLDDLRISADSPLAQAMLGRRVGDEVQVEAPAGPYRCRIVAASDRSDKG
                     DG"
     gene            1685..2092
                     /locus_tag="VE646_02255"
     CDS             1685..2092
                     /locus_tag="VE646_02255"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZD78849.1"
                     /translation="MSPIGQRSLVLILARDLATSLATPVFIVDRSGDLVFYNEAAGRV
                     LGKPFVEGEPMPAERWATAFGQSDDDGTPLGPEAIPLWTAFTEQRPAHRHLVIRGADG
                     VRRRLSVTAFPLYARADEFVGALAVFWETPGDA"
     gene            2098..2934
                     /locus_tag="VE646_02260"
     CDS             2098..2934
                     /locus_tag="VE646_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZD78850.1"
                     /translation="MRIWGCRGSLATPGPQTVRYGGNTSCIEVRLSDDSLVILDAGTG
                     IRPLGLALDGPRPRRIDLLLTHLHLDHLEGLGFFPPLWDPQTELHIWGPPSPLESLQE
                     RIAVYLSPPLFPVHLSDVPSRPIFHDAPEDEVQIGSATVLSQPIIHSGPTVGYRIEDG
                     GSVLAYLTDHEPAHGADLRSTAPEWISGFAIARGADLLIHDCQYTEAEYPAHVGWGHS
                     SLGHVADFARIAKARHLLLFHHDPVRTDDELEVMLARVRELVGNDIEVDLAREGMELD
                     LP"
     gene            complement(2926..3273)
                     /locus_tag="VE646_02265"
     CDS             complement(2926..3273)
                     /locus_tag="VE646_02265"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HZD78851.1"
                     /translation="MGSGDDGWIEVLAASDLPEGKPVKGRADGVDLLLFLQGERIYAV
                     TNRCTHQGAPLHRGIVRRVGQEPVATCPIHGSQFRLSDGAVVRGPAGAPVVAYEARLD
                     GEMVEVRPLSPSG"
     gene            3402..4769
                     /locus_tag="VE646_02270"
     CDS             3402..4769
                     /locus_tag="VE646_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012891154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HZD78852.1"
                     /translation="MDALDLSRWQFAITIAFHFLFVPLSIGLAFLVAVMQTAWYRTEK
                     PVYLRMTKFWGRLFLINFALGVVTGIVQEFQFGMNWSTYSRFVGDVFGAPLAIEGLAA
                     FFLESTFLGLWIFGWERLSRRLHLATIWLVAFGATISALWILAANAWMQHPVGFAMDP
                     ATGRAQMRNFFAMLTNPVLAAQFPHTVLAALTTGGMFVIGVSGYHLLRAREADLFTRS
                     ARIALVVTFVASIGIAFTGHWQAQQMTRYQPMKMAAAEALWDTEQGPGFSLFAVGDIE
                     NGRNHINVQLPHMLSVLATNRWNGTVEGINDLQARFEQEYGPGSYLPVVGLMYWTFRL
                     MVGAGLLMIVLTLAGLWLMRRRSLGRARWFQRAAPIAIALPIAANLLGWTFTEMGRQP
                     WVVYGLLKTSAAGSPSVGTAFVLATLIGFTLIYGLLAGIDVFLLAKYSRTEPAANVGI
                     GAEAY"
     gene            4795..5865
                     /gene="cydB"
                     /locus_tag="VE646_02275"
     CDS             4795..5865
                     /gene="cydB"
                     /locus_tag="VE646_02275"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HZD78853.1"
                     /translation="MNLETLWFGLIAVLWTGYFFLEGFDFGVGTLLPFVGHDDTDRRV
                     LVNAIGPVWDGNEVWLIVAGGATFAAFPGWYATLFSGFYLALFLVLAALIFRGVAFEF
                     RGKDRSVRWKAWWDRAIFWGSAVPALLWGVAWANILRGVPIDARQEFVGSFFDLLNPY
                     ALLGGVMTLLLFTLHGAVFLTLRTEGSLRERTRAVAIRLSWPATAAVLGFLSWTYVNA
                     VEIDNKGVVPGVVPIAAIGLVAAVSWLLHEKLDGWAFLATGLGVVLLTATIFLNLYPR
                     VLVSSLGAANDLTIWNVASSHYTLKVMTIVALTFTPVVLLYQGWTYWVFRRRLGRIDV
                     TGPQGDGEAETRVEEAGFPITP"
     gene            5928..>6646
                     /locus_tag="VE646_02280"
     CDS             5928..>6646
                     /locus_tag="VE646_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HZD78854.1"
                     /translation="MMQPETSFPIGSDRGVGANLQRAREERGVSLSEVAQATRIRPRY
                     LEALENDAPEDVFPGRVYARFFLREYARYLRLPDQPLVEALDARWTVGPPQEETLAPV
                     LALAPMERSRRWPGRLVTLIAVALLATLVSLSVVAGGRGDRAAEPPLGATTGGPSAGS
                     TSGAGSTGGGSATRGHAPPGAGGSVTPPEVTSIRARLVAADRCWVYARAADGRVLFSQ
                     TLEPGMSTSLQARRGLDLQLG"
BASE COUNT          933 a         2270 c         2369 g         1074 t
ORIGIN      
        1 tcaccgcggg cctgcggcgg atgtccggca gggacagctc caccgatcgc tccacgttcc
       61 cctccacggt caaccgccag gccgacgcat cgagcgccgg gacgtcgaaa tgcaccagct
      121 ggtagtgcat gccgacgggc gtgaggtcgt agcgcagagc ctccagcggc atgccctggt
      181 tccgggtggc gagctcgagc tcctcgaagg tgatcccctg gtcgatcgct gccatgtccg
      241 ccacgggacc cagtctcgca caccccaccc agaaccgtcg gatccgaccg tcgcgttgca
      301 caaggatcga gccggtgcca aggtcgttcc agcgcagtcg aggaggtgga gatggagctg
      361 aggagcgaca gcatcagcga gggccaggcg atcccccacc gatacgcgct ggcgacgccg
      421 acgcccgagg ggaaggcgga gctcaccggc gaggacgtca gtccgcacct gcgttggtcc
      481 ggagagcccg agggggtccg gtccttcgcc gtatcggtgg tcgaccccga cgtgccggcc
      541 gatgcctcgc ggatggaggt ggagggcctg gcgctgggcc gggacgagcc cagggtcgac
      601 ttcgcccact ggctcatcgt ggacatccca ccggagctgc acgagatccc cgagggtgcc
      661 gacggcgacc gcttcgtggc ccacggcaaa cctccgggac cgaccggctt cggggggctc
      721 tccggccaga acgggtacac gggtctgttc gccgggagcg aggagcgtgc cggcacgtac
      781 ggaggttggg acggccccta cccgccgtgg aacgacgagg tggtgcatcg ctacgtcttc
      841 accgtgtacg cgctggacgt gccgtccgtg ggcctgcagc cgggcttcac cctcgatgag
      901 ttccgccgag ccatcgacgg acaggtcctc gacaccggcg aactgctcgc cacctacacg
      961 ctaaacccac gactgcgctg agggtgtggg ggagcgcccg accgaagcgg tagactgagg
     1021 ctccgggccg gcgaccgcgg ccgggctgac gccacgcccc cgcccctcgc tcgaggagca
     1081 aaccccgaac gagcagctcg gatgcatggt tcgacgccca gccccaggag ccggtggaag
     1141 gagggagcag cgtgggttcc acacgagaga cgggcaacgg cgtccccagc atgctgaccg
     1201 aggaaggcaa gcgtcggctc gaggagcggg ccaagcggct gcgggaggag gtcattcccg
     1261 acatcgtggc ccggctcgag atgcccgagc gtgatgctca ggtggacgcc gagttccagc
     1321 gggcgaccga cgagctggcc gacatcgact acctgatccg tcacgcgccg gtcgccgagc
     1381 aggttccggg ggacccgcag gtggtcgagg tcggggacga ggtcgaggtc cgcttcgacg
     1441 acggttccac ggaccacttc ctgatcgtgc acccggtgga ggccaccctg gacgatctac
     1501 ggatctcggc cgattcccca ctggcccagg cgatgctggg acgacgggtg ggagacgagg
     1561 tgcaggtgga ggcgccggcc ggtccttacc gctgtcggat cgtggccgcg tcggaccgga
     1621 gcgacaaggg cgacggctag tctcatcgtt gtcccgtcgg gtcggcccgg ctaggctccc
     1681 acccatgagc ccgatcggcc agcgcagcct cgtgctcatc ctggcgcgcg acctggccac
     1741 cagcctggcc accccggtgt tcatcgtgga ccggtccggc gatctggtct tctacaacga
     1801 ggcggcgggc cgggtcctgg gcaagccgtt cgtggagggc gagcccatgc cggccgagcg
     1861 ctgggcgacc gccttcggcc agagcgacga cgacggcacg cccctgggac cggaagcgat
     1921 cccgctgtgg acggcgttca cggagcagcg gccggcccat cgacacctcg tgatcagggg
     1981 ggccgacggt gtccgccgcc gcctgtccgt caccgccttc ccgctgtacg cacgggccga
     2041 cgagttcgtg ggcgccctgg cggtgttctg ggagaccccc ggggacgctt gatgcatgtg
     2101 cggatctggg ggtgccgtgg ctccctcgcg acgcccggac cccagacggt ccgctacggg
     2161 gggaacacct cgtgcatcga ggttcggctc agcgacgaca gcctggtcat cctcgatgcc
     2221 ggcaccggga tccgccccct ggggctggct ctggacggcc cgcgccccag gaggatcgat
     2281 ctcctcctca cccatctgca cctggatcat ctggagggtc tggggttctt cccgccgcta
     2341 tgggatccgc agaccgagct ccacatctgg gggccgccct cgccgctgga gtcgctgcag
     2401 gaacggatcg ccgtctatct gtcgccgccg ctgttcccgg tgcacctgtc cgacgtccct
     2461 tcccgaccga tcttccacga tgcccccgag gacgaggtcc agatcggatc ggccacggtc
     2521 ttgtcccagc cgatcatcca ctccggtccc acggtcggat atcggatcga ggacgggggg
     2581 agcgtcctcg catacctcac ggaccacgag ccggcccacg gtgcggacct gcgcagcacc
     2641 gcccccgagt ggatctccgg gttcgcgatc gcgcggggag cggacctgtt gatccacgac
     2701 tgtcagtaca cggaggcgga gtaccccgcg cacgtcgggt ggggtcactc cagcctgggg
     2761 cacgtcgccg acttcgcgcg tatcgccaag gcccggcacc tgctcctgtt ccaccacgat
     2821 ccggtgcgca ccgacgacga gctcgaggtg atgctggccc gggtccggga gctggtgggc
     2881 aacgacatcg aggtggacct ggcccgagag gggatggagc tcgacctacc ctgagggaga
     2941 gaggggccgg acctccacca tctcaccgtc cagccgcgcc tcgtaggcga ccacgggggc
     3001 gccggccggc ccccggacca ccgcaccgtc gctcaggcgg aactggctgc cgtggatcgg
     3061 acaggtcgcc acgggctcct ggcccacgcg gcggacgatc ccccgatgca ggggggcacc
     3121 ttgatgcgtg caccggttgg tcaccgcgta gatccgttcc ccctggagga agaggagcag
     3181 gtccacgccg tcggctcgcc ccttgaccgg cttgccctcg ggcagatccg atgccgccag
     3241 cacctcgatc cacccgtcgt cgcccgatcc cacgccggcc ccctggtcgt ccggacgact
     3301 ctacccggtc gctcgggccg gcgagagcgg gacccccggc ccggccagcg ggccggcgca
     3361 tccaggacga tgtgcgcggg tcgcaggagg aggagggccg gatggacgct ctcgacctgt
     3421 cgcggtggca gttcgccatc acgatcgcgt tccacttcct gttcgtcccg ctatcgatcg
     3481 gcctggcctt cctggtcgcc gtgatgcaga cggcctggta ccggaccgag aagcccgtct
     3541 acctgcggat gacgaagttc tggggacggc tcttcctgat caacttcgcc ctcggcgtgg
     3601 tcaccgggat cgtccaggag ttccagttcg ggatgaactg gtccacgtac tcgcgcttcg
     3661 tcggtgatgt gttcggggcg cccctggcga tcgaggggct ggcggcgttc ttcctcgagt
     3721 cgaccttcct gggcctatgg atcttcggct gggagcggct gtcccgacgc ctgcacctgg
     3781 ccacgatctg gctggtggcg ttcggggcga cgatctcggc gctgtggatc cttgcggcga
     3841 acgcctggat gcagcatccg gtgggcttcg cgatggaccc ggcgacgggg cgggcgcaga
     3901 tgcggaactt cttcgccatg ctgacgaacc cggtgctggc cgcccagttc ccccacacgg
     3961 tgctggccgc cctcaccacc gggggcatgt tcgtgatcgg cgtctccggc taccacctgc
     4021 tgcgggcgcg ggaggcggat ctgttcacgc ggtccgctcg gatcgcgctg gtggtcacgt
     4081 tcgtggcctc catcgggatc gccttcacgg gccactggca ggcccagcag atgacgaggt
     4141 accagccgat gaagatggcg gcggccgagg ccctgtggga cacggagcag gggcctgggt
     4201 tctcgctgtt cgccgtcgga gacatcgaga acgggcgcaa ccacatcaac gtgcagctgc
     4261 cgcacatgct ctcggtgctg gccacgaacc ggtggaacgg caccgtcgaa gggatcaacg
     4321 acctccaggc gcggttcgag caggagtacg gaccgggcag ctacctgccg gtggtgggcc
     4381 tgatgtactg gaccttccgc ctcatggtgg gggccggtct gctgatgatc gtgctcacgt
     4441 tggccggcct gtggctgatg cgtcggcgct cgttgggacg ggcccggtgg ttccagcggg
     4501 cggcgccgat cgccatcgcg ctgcccatcg ccgccaacct gttggggtgg accttcacgg
     4561 agatgggaag gcagccatgg gtcgtctacg ggctgctgaa gacctccgcc gccggatccc
     4621 cctcggtggg cacggcgttc gtgctggcga ccctgatcgg gttcacgttg atctacggcc
     4681 tcctggccgg catcgacgtg ttcctgcttg ccaagtactc gaggaccgag ccggcggcga
     4741 acgtgggcat cggcgccgag gcgtactgag gtgacggcga tggaacacgg ctacctgaac
     4801 ctggagaccc tgtggttcgg cctgatcgcg gtcctgtgga ccgggtactt cttcctggag
     4861 ggcttcgact tcggggtggg gacgctgctc ccgttcgtgg gccacgacga cacggatcgc
     4921 cgcgtcctgg tcaacgccat cggtccggtg tgggacggga acgaggtgtg gctcatcgtg
     4981 gccgggggag ccacgttcgc ggccttcccc ggctggtacg ccacgttgtt cagcggcttc
     5041 tacctcgccc tgttcctggt gctggccgca ctgatcttcc ggggagtggc cttcgagttc
     5101 cggggcaagg accgcagcgt ccggtggaag gcctggtggg accgggccat cttctggggc
     5161 agcgcggtcc cggcgctgct gtggggtgtg gcctgggcga acatcctgcg gggcgtgccg
     5221 atcgacgccc gacaggagtt cgtggggtcg ttcttcgacc tgctgaaccc gtacgcgctg
     5281 ctgggcggcg tgatgaccct cctgctcttc accctccacg gggccgtgtt cctgacgctc
     5341 cggaccgagg gatcgctgcg cgagcgcacc cgggccgtcg cgatccggct gtcctggccg
     5401 gccacggccg ccgtgctggg gttcctgtcc tggacctacg tgaacgcggt ggagatcgac
     5461 aacaaggggg tcgtgcccgg cgtggtgccg atcgccgcca tcgggctggt ggcggcggtc
     5521 tcctggctgc tgcacgagaa gctcgacggc tgggccttcc tggccaccgg gctgggggtc
     5581 gtcctgctca ccgccacgat cttcctgaac ctctacccgc gcgtcctggt ctcgagcctc
     5641 ggggccgcca acgacctaac gatctggaac gtcgcctcca gccactacac gctgaaggtg
     5701 atgacgatcg tggcgctgac gttcacgccg gtagtactcc tgtaccaggg ctggacctac
     5761 tgggtcttcc gtcgacggct gggccggatc gacgtgaccg gtcctcaggg cgacggtgag
     5821 gcggagacca gggtggagga ggcgggtttc cccatcacgc cttgaccttc gcggccctgg
     5881 ttcggatact gggcgggaca accgatcccc gtggccaatc gggttccatg atgcagccgg
     5941 agacgtcctt ccctatcggc tccgaccgcg gtgtcggagc caacctgcag cgcgctcgag
     6001 aggagcgggg ggtttcgctg tccgaggtcg cccaggcgac ccggatccgc ccccgctacc
     6061 tcgaggcgct ggagaacgac gcccccgagg acgtgttccc cgggcgcgtc tacgctcggt
     6121 tcttcctccg cgagtacgcc cgataccttc gcctacccga tcagccgctg gtggaggcgc
     6181 tggacgctcg ctggacggtg ggtcctcccc aggaagaaac cctcgcgccg gtcctggccc
     6241 tggccccgat ggaacggtcg aggcggtggc cgggtcgtct ggtgacgctg atcgcggtgg
     6301 cgctcctggc caccctggtc tccctctcgg tggtggccgg cggccggggt gaccgggccg
     6361 cggagccgcc gctgggcgcg acgaccggcg gtccgagcgc cgggtccacg agcggcgcgg
     6421 gatcgaccgg cggcgggtcc gccacgaggg gccatgctcc gcccggtgcg gggggttcgg
     6481 tgacccctcc cgaggtgacc tccatccggg cccggctggt ggcggccgat cgatgctggg
     6541 tgtacgcgag ggccgccgac gggcgggtgt tgttcagcca gaccctcgag cccggcatga
     6601 gcacctcgct gcaggctcgc cgtgggctcg atctgcaact ggggaa
//
LOCUS       DASQQQ010000050        13467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_360274, whole genome shotgun sequence.
ACCESSION   DASQQQ010000050 DASQQQ010000000
VERSION     DASQQQ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13467
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360274"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..919)
                     /gene="hpnD"
                     /locus_tag="VE646_02285"
     CDS             complement(53..919)
                     /gene="hpnD"
                     /locus_tag="VE646_02285"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014628571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HZD78855.1"
                     /translation="MSAHASAPEREVQAAYRTCGRITRSEAANFYYGIRLLPADRYRG
                     LCAVYALARRIDDIGDGDLPAEVKLEALERVRDDLERVGASPQDPVLVAVGDAAARFG
                     LPMDAFVDLIDGVEMDVRGTSYRHIDDLVGYCRRVAGSIGRLSVAVFGADDSASAAVL
                     GDDLGVAMQLVNILRDVREDRGVGRCYLPAEDLAAFGLDPRFDLADPAAVALVAFEAG
                     RARGWFERGLQLLPMLDRRSAACVGAMTGIYRRLLTTIEEAPAEVLRRRLSLSPLQKA
                     WVAARCLSGVGG"
     gene            complement(916..1758)
                     /gene="hpnC"
                     /locus_tag="VE646_02290"
     CDS             complement(916..1758)
                     /gene="hpnC"
                     /locus_tag="VE646_02290"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019330379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HZD78856.1"
                     /translation="MGATAVPRPDAVMARVQGENFPVASRLLPAEVRRDLLAIYGFAR
                     LVDDAGDEAAGDRAALLDHLEREVDLLFAGAPTHPLFRRLVPTVRRHRIPDQPFRRLI
                     AANRQDQTVRRYPTFSDLRAYCELSANPVGHLVLHVLEAATPERVALSDDVCTGLQLV
                     EHWQDVREDHGRGRIYVPQDDLVRFGVPEAILGSDVPTEAFHALMAFEEGRARSLLAS
                     GAPLAATFRGRARYAVAAFAAGGLAALDAMARADHDVLSGSPRPGALRRARWLLAVLR
                     GGAG"
     gene            complement(1758..2210)
                     /locus_tag="VE646_02295"
     CDS             complement(1758..2210)
                     /locus_tag="VE646_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HZD78857.1"
                     /translation="MTVLLENHSFQTTHCGQFLDITDDVREVVTKSGVQNGSVLVYSP
                     HTTCSVVINESESGFVQDFCRLMDSLVPVDGSYVHDDLEVRTQNLEDDPHDIPNGWAH
                     CRQSLIGSASQHIPVKDGEPLLGRWQRVFFLELDRSRDRKVFIQAMGE"
     gene            complement(2363..3175)
                     /locus_tag="VE646_02300"
     CDS             complement(2363..3175)
                     /locus_tag="VE646_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZD78858.1"
                     /translation="MAAHDRFTIAELTRRTGVPPATIHHYLRLGLLAPPKRAAVNRFL
                     YDDRHVTGLRLIRVLRERRGLSLKTIRRVLPDLVALEAGEAFRPEMWDRAIDLRMGPD
                     RRRTPGGRLLDAAVESFGRSGYGDVNVDDICRVARIAKGSFYRHFRSKEELFFAAAEA
                     AAAQVTQAVAESVGDGPVPIDRLASWAADLMEPRSALFLDLLGRASQRRPGYPAAARR
                     VFGDLMLGLGRHTDPADPERAGGEVLDRVLSTLLRRALNPDAVPLPLPARRR"
     gene            3216..4073
                     /locus_tag="VE646_02305"
     CDS             3216..4073
                     /locus_tag="VE646_02305"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZD78859.1"
                     /translation="MGIGPGVLTMGTVGLVTDSSACLPAQIADRAGIGVVPVTIHLSS
                     RVVPGDDPVAARLVYDALRRDEPVKSSAPTPLEYLQAIDDLEVDSVIVVVPAEEFTVM
                     RRNAMVGAGLARHPVTVVDSRTAASAQGLVVGAAAAAIAAGGSHDDVVEVIEDASRRA
                     ELVAMLDQLRYVRRSGRVSAAALGLAEELGIKPVFRLRDGRVERVAIPRSEGAALERI
                     AREAARHGLPQGDGAVVFHASSPARADRLVGRLGVTAPPVEFSPGMGIHTGPGVAGVA
                     WLRRSSSQA"
     gene            4070..5713
                     /locus_tag="VE646_02310"
     CDS             4070..5713
                     /locus_tag="VE646_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78860.1"
                     /translation="MTPSRSRRFTRLLLPCALAPMLETGLVAELGPRSGLALAPQVTA
                     LPPFDLFHDLRWISVYHESWILLALELLAMIGVRAVWVAWTVRAAWPGSDPPEVRRAA
                     VRLLLYETIAATLMIPWVALLFGLALTHLSFLFFAAIPPALGIAVAIHRGAVSRAAGP
                     GYRWGPTSASLGWVLGSFMWLSVAGAAAGSWSTGPAVAVAGLAGLGNARAWIGVVEDL
                     AGAPHRASGRWVASSLVAATFAVVLVGTAAGFAADGSVSPPVRAGQTGTPMAATVHSV
                     LVARGLFSRLGPSTPVLGAGLPPGTAVRAFSYRGLDEAGEPRPYGPSDTLRTPEAMAR
                     LMDRQVRALYRAYGRPVTIVAESEGALIARTYLLTRYPRHSGMVDELVTLGLAVGPTA
                     VYFPPPGTQGWGVGSGWALRGLAGVLRGLGPLDFTPDAPILRRLTDCSSMFRRVVDER
                     LPGGVRQVSIGALADAVDPSGENPAGPGWIVAAAHGGMVGRPDVQRLIADVLEGRAPP
                     AGSVWGRTASTLVSATSAAWRVPSLLPQLRPSARCGTVR"
     gene            complement(5753..6250)
                     /locus_tag="VE646_02315"
     CDS             complement(5753..6250)
                     /locus_tag="VE646_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78861.1"
                     /translation="MSAAALSPRSIRRRRRGLILGLTVLMGAVAALPAIPWLMGIVTN
                     AKSSYTAEIPAENRGTYVVTPDGVTQLYSWSILLDQMPADAPVLERADVDMFAVVQKQ
                     FDRLSNYRLIHLADDAEIPLRLVSERGMQLLLRPERLAAGAYLFVVPSDSMFGGSTYQ
                     YFRIR"
     gene            complement(6247..8229)
                     /locus_tag="VE646_02320"
     CDS             complement(6247..8229)
                     /locus_tag="VE646_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZD78862.1"
                     /translation="MHIEHITNLQAVLFVGGVATLAYVVFRVIPGVNVERPRTSYRSE
                     EITAYDRALPKYFVAAVAAVAIGAIHAVIKDIPPLYEWLALAGHGGHLIRDIANTHLV
                     IVLGGTVAATGLTWYVLPRMTGRPLYSERLASLSFWCTLIGAGGFYLANVALGIVLGR
                     MARQGIPYDVAKSQLGVWRAVPIGVSASVMGIGYWTFVADVVITAWVSRRVETPRPHA
                     HLIKYFVIGALGLFAGTVQGVIQVMPSQEAWIRAAVPAGHYIDPIAHAHVNLVTGMLS
                     LIAGLAFFWLRDEDAERGRRREQLVFWTLVPGSLMFYATFLFLGFSEGRLIVDHGLSF
                     EQAMARMGPLHVVPLMVSGIVTLTGVWLLLGTILIRVLFGHARRAAGSAFVLLGIAAL
                     FVGTAQGLIQLLPPVKLWLEAAGQPGDAIANAHAQLNILGGVLSLLVGLLFLGGVGFA
                     GRVPRPEVTRRIAGFAAVGAGTYYLSAMATAVVLGRSVASGRPLGRSLGLALPWGPLG
                     MALGAALYAVAFVRLSGFGWSATTAYRKAGWAEFLRRLAADEGSSPRWRQRIPVVYYL
                     AAELLSGSVGFPGLGWILSGRSTVGLPMALGGPFLAWAMIPLVTSPRGAGTLSHHTIV
                     ADALYLSISTLTSLLVLWWIEAREQRIRRLEARLTG"
     gene            complement(8233..8466)
                     /locus_tag="VE646_02325"
     CDS             complement(8233..8466)
                     /locus_tag="VE646_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78863.1"
                     /translation="MADEGDERERDQGRPGAPRGDEAGEEKTARWDREVERIEQERWT
                     TPNQIKDWLVLLLMMVVFIGWGLGVYFFEPGLR"
     gene            complement(8482..8787)
                     /locus_tag="VE646_02330"
     CDS             complement(8482..8787)
                     /locus_tag="VE646_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78864.1"
                     /translation="MYPESYTHLIAGSIPRDTWQEAWYSISTWKGYLQSFPGFLRLRV
                     SIRELEGGDIRVAIATIWEHEEQLDAWLGGQWRADRILQQLSTPAYDVTDEILADLS"
     gene            complement(8791..9234)
                     /locus_tag="VE646_02335"
     CDS             complement(8791..9234)
                     /locus_tag="VE646_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78865.1"
                     /translation="MEHDTGAAQAHVLHDLNPQVTISTPSGVKVVRNVPTGNDTAASQ
                     AYYDYLLGRRMTDIPPTRTGIQWQGPIIIYSWFLALTVFFFLFARFFSIAHRKRNELY
                     GVTRFGGGILERNGRVTWFTWGVIAALTALGIWYVVFQSVHGQWY"
     gene            complement(9224..9526)
                     /locus_tag="VE646_02340"
     CDS             complement(9224..9526)
                     /locus_tag="VE646_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78866.1"
                     /translation="MKTIRRVLSVLLLSGVVVLATAGPSFAHDAPDQGQAAWLMADWM
                     LLSFLVFFLAALFGFVIAVRRGYLRNLEQAKYHILTIDEEDYYTPDWAREEVTDGA"
     gene            complement(9541..10980)
                     /locus_tag="VE646_02345"
     CDS             complement(9541..10980)
                     /locus_tag="VE646_02345"
                     /inference="COORDINATES: protein motif:HMM:NF014486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HZD78867.1"
                     /translation="MAEHIPGEFQEPRSAIDYTREGPRRRLLMTPHLAAIGGLLAFFT
                     VVLMVVVLPTETYHPPASTNWLPLSNLALRGRAVYLSNGCVYCHSGFSRPQDVAVGLY
                     YLYPRVSEPGDYAGNDSPNILGSERTGPDLSQEGGNHPDDWQRAHYDDPRNTTPISIM
                     PRFNFFSDSQLKDMIAFNQSQGGKEATLRNSAILVGKALWNGGVEGTLKDLANVFPTE
                     VSQAQSAGTYHADGTIDDTTKWGLSWGDVWAVNTHLRSYWVTRNPLPPTEDNLVIGKQ
                     IYLTRCSGCHGPTGDGNGPGARFMAPKPADFIHVDDQMTAPDASDGQFYYRVLTGGKG
                     TDMENFGTRLSVEDTWRVVLFLETIAKGGLKQTLPTTDMFIKWEPQQGLLNYIKDHPV
                     SDPNAGYDPTAGPFEQAARWIAPGLAPQDTVYIAGKLPVNLATIAKMVEERYRQFVQQ
                     DYQDALGRGEPMPPRSEIFSTEGVQWHAP"
     gene            complement(10973..12505)
                     /locus_tag="VE646_02350"
     CDS             complement(10973..12505)
                     /locus_tag="VE646_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZD78868.1"
                     /translation="MAVTTGTRNGRGIGFVSPEVLQVDEPVSEYDDRAAKIWLFSSIF
                     WLTFVDLVGLTMATELVTPDLFGGIPWLLFSRIRPIHVNGVIFAWLSSMYFGGIFYVL
                     PRLTGVRSMWSERTAIWMAWAWNAMFTAGVITLALGYTQGREYAEFIWPIDVVLVAIW
                     LVNIYNIVMTVLNRRIKPLYVTVWWFLGSPLWLVADYIIGNVMWRPGSMLGNGVPGGH
                     ISGALTNPLADGMLNWWQNHNLFGLWLTPMLVALTYYIVPRVTNTPLYSHTLSLISFW
                     GIAFFYTGVGHHHLLQAPIPGWLKTFATVNSIFLLIPVFAFIANITLTMRGNWEKVFT
                     NLPLRFTITAFIFYDLVNIQGTFMALPSFNRVIHFTNFIVAHAHLALLGAFTILGMGV
                     IDYVIPQIYKRPIYSRSLVEWQYWLILIGFTGFFVSLTFASFLQGQNWALGIPEVNVL
                     PEIRPHYIARGVFGAMIVMSGIVQIYNIWRTVRGDTHQKTRDEIEPFLRAGEPAEATA
                     RG"
     gene            12774..>13467
                     /locus_tag="VE646_02355"
     CDS             12774..>13467
                     /locus_tag="VE646_02355"
                     /inference="COORDINATES: protein motif:HMM:NF017051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78869.1"
                     /translation="MSGSRMEPKCEVPIQVVGAGYPRTSIRTLERLVREGSDVYRRLR
                     HAGIDDAVVLSTCQRLEIYGASPHAPAHAGLPSLERIVEPATEGPPASDLFELSGSAA
                     VEHLFSVAAGIESILPGEPQILTQVREALERGEAERMAGPALRRLFHAALRAGRRVRR
                     EAGIGLAHDAVVQAAIDLAMDLPGHARPQVTAVVGAGRMARIAVQHLARLGLNVRTVL
                     DRTPERASDLAAG"
BASE COUNT         2132 a         4720 c         4619 g         1996 t
ORIGIN      
        1 cccgccagcc caccaccgac caccaccacc cgaccgctgc cgtccatcga gctcaccccc
       61 ccacccccga caggcagcgg gccgccaccc acgccttctg gagcggcgag agggacagtc
      121 gccggcgcag gacctcggcc ggcgcctctt cgatcgtggt caggagccgg cggtagatgc
      181 cggtcatggc tccgacgcag gcggcgctgc gccgatccag catcggcaag agctgcaggc
      241 cccgctcgaa ccacccgcgg gccctcccgg cctcgaaggc gaccagcgcc acggccgccg
      301 ggtccgccag gtcgaacctc gggtccaggc cgaaggcggc taggtcctcg gccggtaggt
      361 agcagcgtcc gacgccccga tcctcccgga cgtcgcggag gatgttcacg agctgcatgg
      421 ccacacccag gtcgtcaccg aggacggccg cgctcgcgga atcatcggcg ccgaacaccg
      481 ccaccgagag gcgtccgatc gacccggcca cccgccggca gtagccgacc aggtcgtcga
      541 tgtgtcggta cgaggtgccc cggacgtcca tctcgacccc gtcgatcagg tcgacgaagg
      601 catccatcgg caggccgaag cgcgcggcgg cgtctcccac cgccacgagc accgggtcct
      661 ggggggaagc gccgacgcgc tcgaggtcgt cgcgcacgcg ctccagcgcc tcgagcttga
      721 cctcggccgg gagatctcca tccccgatat cgtcgatccg gcgggccagg gcgtagacgg
      781 cgcagagccc tcggtaccga tcggccggca gcagccggat gccgtagtag aagttcgccg
      841 cctcggaccg tgtgatccgg ccgcacgtcc ggtaggcggc ctggacctcg cgttccgggg
      901 cagaggcgtg agcactcatc cggccccacc tcgcaggacg gcgagcagcc accgggcacg
      961 tcggagcgct ccgggacggg gtgagccgga gagcacgtcg tgatccgcac gcgccatcgc
     1021 gtccagggcg gccagccccc ccgcagcgaa cgcggccacc gcgtaccggg cccgaccccg
     1081 gaacgtcgcc gcgagcggcg cgccgctcgc cagcagcgag cgagcccgtc cctcctcgaa
     1141 ggccatcagg gcgtggaagg cctcggtggg gacgtcggag cccaggatcg cctcgggcac
     1201 accgaaccgc accagatcat cctgggggac gtagatccgt cctcggccgt gatcctcgcg
     1261 gacgtcctgc cagtgctcca cgagctgcag tccggtgcac acatcgtcgg acagcgccac
     1321 acgctccggc gtggccgctt ccaggacgtg cagcacgagg tgacccaccg ggttggccga
     1381 gagctcgcag taggcccgga gatcggagaa cgtggggtac cggcggacgg tctgatcctg
     1441 ccggttggcg gcgatcagcc ggcggaaggg ctggtccggg atccgatgcc gccgcaccgt
     1501 gggaaccagc cgccggaaca gtgggtgcgt cggggctccc gcgaacagca ggtcgacctc
     1561 acgctccagg tggtcgagca gggcggcacg atccccggca gcctcgtccc ctgcatcgtc
     1621 caccagcctg gcgaagccgt agatcgccag caggtccctc cgcacctccg ccggcagcag
     1681 gcgcgaggca accgggaagt tctcgccctg gacgcgagcc atgaccgcgt cgggccgcgg
     1741 cacggccgtc gcgcccatca ctcccccatc gcctggatga acacctttcg atctcgggag
     1801 cggtcgagct ccaggaagaa gacccgctgc cagcggccga ggagcggctc gccatccttc
     1861 acggggatgt gctgagaggc cgacccgatc agcgattgcc ggcagtgcgc ccacccgttg
     1921 gggatgtcgt gggggtcgtc ctccaggttc tgggtccgga cctccaggtc gtcgtggacg
     1981 taggagccgt ccaccgggac cagcgagtcc atcaggcggc agaagtcctg cacgaatccc
     2041 gactcgctct cgttgatcac cacggagcag gtcgtatgcg gggagtagac aaggacgctg
     2101 ccgttctgca ccccgctctt ggtgacgacc tcacgcacgt catcggtgat gtccaggaac
     2161 tgcccgcagt gggtcgtctg gaacgagtga ttctcgagta gaacggtcat cccacctcca
     2221 caagcccgtt gcctgcagac ctttccccct cgcggtgcga ctcacacata tctcacatgc
     2281 ctgaccgatc ggtctagtcc gttgttggtg gctaccgatc atccgaggga tcgtggccgc
     2341 atgtcaagcg gcgaatgccg gctcaacgcc ggcgcgcggg gagcggcagc gggaccgcat
     2401 ccgggttcag cgctcgacgg agcagcgtgc tcagcacccg atccaggacc tctcccccgg
     2461 cccgctcggg atcggccggg tcggtgtgcc ggccgagccc gagcatgagg tcgccgaaga
     2521 cgcgacgcgc ggccgccggg tatccggggc gacgttgaga ggctcgcccc aacaggtcca
     2581 ggaacagggc cgatcgtggc tccatgagat cggcggccca cgacgccagc cgatcgatcg
     2641 gcacgggacc gtcgcccacc gactcggcga ccgcctgggt cacctgagcc gccgccgcct
     2701 cggccgcggc gaagaacagc tcttccttgg agcggaagtg ccggtagaag gaccccttcg
     2761 cgatcctggc cacccggcag atgtcgtcga cgttcacgtc cccgtatccg ctccgaccga
     2821 aggactcgac ggccgcatcg agcaggcgcc cgcccggggt ccgacggcgg tcgggtccca
     2881 tccgcaggtc gatggcccgg tcccacatct ccggccggaa ggcctcgccc gcctcgagcg
     2941 ccaccagatc gggcaggacc cggcggatcg tcttcaggga caacccccga cgctcccgga
     3001 gcacccggat cagtcgaaga ccggtgacgt gccggtcgtc gtagaggaac cggttgacgg
     3061 cggcccgctt gggcggcgcg agcagcccca gccggaggta atgatggatc gtcgcaggcg
     3121 gaacgcccgt cctgcgcgtc agctcggcga tggtgaatcg gtcatgtgca gccatctgaa
     3181 cccaacggtc gatagttaca tacaatcatc ctcacgtggg tatcggacct ggggtgctga
     3241 ccatgggaac cgtagggctg gtgacggact ccagcgcatg ccttccggcc cagatcgcgg
     3301 accgggcggg tatcggcgtg gtgccggtca cgatccacct ctcctcccgc gtcgtcccgg
     3361 gcgacgatcc cgtggcggcg cgactcgtct acgacgccct ccgcagggac gagccggtca
     3421 agtccagcgc ccctaccccg ctcgagtacc tccaggccat cgacgacctg gaggtggact
     3481 cggtgatcgt ggtggtgccc gccgaggagt tcacggtgat gcgtcggaac gccatggtgg
     3541 gggccggtct ggcgagacac cccgtcacgg tggtggactc ccggaccgct gcctcggccc
     3601 aggggctcgt ggtgggagcg gcggcggccg cgatcgccgc cggtggctcc cacgacgacg
     3661 tggtcgaggt catcgaggac gcctcccggc gggccgaact cgtggccatg ctcgatcagc
     3721 tccggtacgt ccgccgcagc ggccgcgtgt ccgctgcggc cctgggcctc gccgaggagc
     3781 tggggatcaa gccggtcttc cgtctccggg acggccgcgt ggagcgggtc gcgatccctc
     3841 gcagcgaggg agcggccctg gagcgcatcg ctcgggaagc cgcgcgccac ggtctgccgc
     3901 agggtgacgg cgcggtagtg ttccatgcga gctccccggc ccgggcggac cgcctggtgg
     3961 gccgcttggg cgtgacggcc ccgccggtcg agttcagtcc aggcatgggc atccacaccg
     4021 gcccaggtgt ggccggggtg gcctggctac ggcgttcttc ctcccaggcg tgaccccctc
     4081 acgctccaga cgcttcaccc gcctgctgct gccctgcgcg ctcgcgccga tgctcgagac
     4141 ggggctcgtc gccgagctgg gaccgcggag cgggctggcg ttggcccctc aggtcacggc
     4201 gctgcccccc ttcgacctct tccacgacct gcggtggatc tcggtctacc acgagtcctg
     4261 gatcctgctg gccctcgagc tgctggcgat gatcggggtc cgggcggtct gggtggcgtg
     4321 gaccgtgcgg gccgcctggc cggggagcga cccgcccgag gtgcgacggg cggccgttcg
     4381 gctgctcctg tacgagacga tcgccgccac cttgatgatc ccctgggtcg ccctcctctt
     4441 cgggctggcc ctcacccatc tctccttcct gttcttcgcc gcgatccccc ctgcgctggg
     4501 tatcgccgta gcgatccatc gtggagcggt gagccgggcg gcgggtcccg ggtaccggtg
     4561 gggtccgacc agcgcgagcc tgggctgggt cctcggttcc ttcatgtggc tcagcgtggc
     4621 cggtgccgcc gccggctcgt ggtccaccgg accggccgtg gcggtcgcgg gcctggccgg
     4681 tctgggcaac gcacgggcct ggatcggcgt ggtcgaggac ctggcggggg caccgcaccg
     4741 ggcctccggt cgctgggtgg cctcctccct ggtggccgcg accttcgccg tggtgctggt
     4801 cgggaccgcg gccgggttcg ccgccgacgg atcggtgtct ccgcccgtac gggccggcca
     4861 gaccgggacc cccatggcgg ccacggtcca ctcggtgctc gtggcccgag ggttgttcag
     4921 tcggctcggt ccatccacgc cggtgctggg agcgggactc ccacccggaa ccgccgttcg
     4981 ggcgttctcc tatcggggcc tggacgaggc cggtgagcca cgtccctacg gcccctcgga
     5041 cacgcttcga acccctgaag cgatggccag gctcatggat cgccaggtgc gtgccctgta
     5101 ccgcgcctac ggtcgcccgg tcacgatcgt ggccgagagc gagggagcgt tgatcgctcg
     5161 gacctatctg ctcacccgct atccgaggca cagcggcatg gttgacgagc tcgtcacgct
     5221 cggcctcgcg gtcggcccca ccgccgtgta cttcccccct ccggggacgc agggttgggg
     5281 cgtgggcagc ggttgggccc tgcgaggtct ggcaggcgtg ctccggggcc tcggcccgtt
     5341 ggacttcacc cccgatgcgc cgatcctccg tcggctcacg gactgtagct cgatgttccg
     5401 ccgggtggtg gacgagcggc tgcccggagg ggttcggcag gtctcgatcg gggcgctggc
     5461 ggacgcggtg gaccccagcg gcgagaaccc cgccggtccc ggctggatcg tggccgccgc
     5521 ccacggaggc atggtggggc ggcccgacgt acaacggctg atcgccgacg tgctcgaggg
     5581 ccgggcacct cccgccggct cggtgtgggg tcgaacggcc tccaccctgg tgtcggccac
     5641 ctcggccgcc tggcgggtgc cctcgctgct cccgcagctg cgccccagcg cccgatgcgg
     5701 caccgttcgc tgagggtccc cggagacgag cgcgccccgg ccaccgatgg cctcagcgga
     5761 tgcggaagta ctggtaggtg ctgcccccga acatgctgtc cgagggcacc acgaacaggt
     5821 acgcgccggc ggccagccgt tccggacgaa ggagcagctg catcccgcgc tcggaaacga
     5881 gccggagtgg gatctccgcg tcatcggcca ggtggatcag ccggtagttc gacaggcggt
     5941 cgaactgctt ctggacgacc gcgaacatgt ccacgtcggc gcgttcgagc accggggcgt
     6001 cggccggcat ctggtccaac aggattgacc aggagtacag ctgcgtcacg ccgtcgggcg
     6061 tgaccacgta ggtgccccgg ttctcggccg ggatctcggc cgtgtagctg ctcttggcgt
     6121 tggtgacgat gcccatcagc caggggatcg cggggagcgc ggcgaccgct cccatgagca
     6181 ccgtgaggcc gaggatcaga ccccgccggc ggcgtcggat cgatcggggc gacagggcgg
     6241 cggcgctcat ccagtcaacc gagcctcgag tcggcggatg cgctgctccc gcgcctcgat
     6301 ccaccacagc acgagcagcg aggtcagcgt gctgatgctg aggtacaggg catcggccac
     6361 gatcgtgtgg tgggacagcg tccccgcgcc tcggggtgag gtgactaggg ggatcatggc
     6421 ccaggccaag aagggacccc caagcgccat gggcagaccg acggtggagc gaccgctgag
     6481 gatccacccc aggccgggga agcccaccga gcccgacagc agctcggccg ccaggtagta
     6541 gacgacgggg atccgctggc gccagcgggg gctactgccc tcgtccgccg ccaggcgccg
     6601 caggaactcg gcccagcccg ccttccggta ggcggtggtg gccgaccacc cgaacccgga
     6661 caggcgcacg aacgccaccg cgtacaaggc ggcgcccagg gccatgccga gcggtcccca
     6721 gggcagcgcc agccccaggc tccgcccaag cggccgaccg ctcgccacgg agcggcccag
     6781 gacgacggct gtcgccatgg ccgacaggta ataggtacca gctccaacgg cggcgaaccc
     6841 ggcgatcctc cgggtcacct cggggcgggg aacccgcccg gcgaacccca ccccacccag
     6901 gaacagcaga ccgaccagca gggacaggac gccgcccagg atgttgagct gggcgtgggc
     6961 gttggcgatc gcgtcgcccg gctgccccgc ggcctccagc cacagcttca ccggggggag
     7021 cagctggatc agcccctggg ccgtgcccac gaacagggcg gcgatcccca gcaggacgaa
     7081 ggcggaaccg gcggcccgac gggcgtgccc gaagagcacg cggatcagaa tcgtccccag
     7141 caggagccac accccggtga gggtgacgat gcccgagacc atcagcggaa ccacgtgcag
     7201 ggggcccatc cgggccatgg cctgctcgaa agacaggcca tgatcgacga tcagcctgcc
     7261 ttcggagaac cccaggaaca ggaaggtcgc gtagaacatc agcgacccgg gcaccagcgt
     7321 ccagaacacc agctgctccc gccgccgccc ccgctcggcg tcctcgtctc ggagccagaa
     7381 gaaggccagc cccgcgatca gggacagcat gccggtcacc aggttcacgt gggcgtgggc
     7441 gatcggatcg atgtaatggc cggccgggac cgccgcgcgg atccaggcct cctggctcgg
     7501 catcacctgg atcacgccct gcacggtccc cgcgaagagt cccagagcgc cgatcacgaa
     7561 gtacttgatc aggtgggcgt gcggcctcgg tgtctccacc cgccgcgaga cccaggccgt
     7621 gatcaccacg tccgccacga aggtccagta gccgatcccc atcaccgacg cggacacccc
     7681 gatcggcacc gcccgccaga cgcccagctg gctcttggcc acgtcgtagg ggatcccctg
     7741 ccgagccatc ctgccgagca cgatgcccag ggcgacgttg gccaggtaga agccaccggc
     7801 cccgatcagc gtgcaccaga acgacagcga ggccagccgc tcgctgtaca gcggccgccc
     7861 cgtcatgcgc ggcagcacgt accaggtgag cccggtcgcc gcgaccgttc cccccagcac
     7921 gatcacgagg tgcgtgttgg cgatgtcccg gatcaggtgc cctccgtggc cggccagcgc
     7981 caaccactcg tacaggggcg ggatgtcctt gatcacggcg tggatcgcgc cgatcgccac
     8041 ggccgccacc gccgccacga agtacttggg gagcgcccgg tcgtacgcgg tgatctcctc
     8101 cgaccggtac gaggtgcgag ggcgctccac gttcacaccg gggatcacgc ggaagacgac
     8161 gtaggcgagc gtcgccaccc cccccacgaa gaggacggcc tgcaggttgg tgatgtgctc
     8221 gatgtgcacg gctcagcgca ggccgggctc gaagaagtac accccgaggc cccagccgat
     8281 gaagacgacc atcatcagca gcaggacgag ccagtccttg atctggttcg gggtcgtcca
     8341 gcgttcctgc tcgatgcgct cgacctcccg gtcccaccgg gcggtcttct cctcaccggc
     8401 ttcatcaccg cgcggggccc ccggacggcc ctgatctcgt tcgcgctcgt cgccctcgtc
     8461 ggccatcccg gtcctcctcc ttcagctcag atcggccaag atctcgtccg tgacgtcgta
     8521 cgcgggggtg gacagctgct gcaggatccg atcggcccgc cattgaccgc ccagccaggc
     8581 atccagctgc tcttcgtgct cccagatcgt ggcgatggcc acccggatgt cgcccccctc
     8641 cagctcgcgg atgctgaccc ggagccggag gaagccgggg aacgactgca ggtagccctt
     8701 ccacgtggag atcgagtacc aggcctcctg ccaggtgtcc cgagggatcg agcccgcgat
     8761 gagatgggtg tacgactcgg gatacatggc tcagtaccac tgcccgtgga cggactggaa
     8821 gacgacgtac cagatgccga gcgccgtgag ggcggcgatc accccccacg tgaaccaggt
     8881 gacccgtccg ttccgctcca agatcccgcc gccgaaccgg gtcaccccgt acagctcgtt
     8941 gcgcttgcgg tgagcgatgc tgaagaacct ggcgaagagg aagaagaaca cggtcagggc
     9001 caggaaccag gagtagatga tgatcggacc ctgccactgg atcccggtcc gggtcggcgg
     9061 gatgtccgtc atccgccgcc ccaggaggta gtcgtagtag gcctgggagg ccgcggtgtc
     9121 gttgccggtg gggacgttgc ggaccacctt caccccgctg ggggtggaga tcgtcacctg
     9181 ggggttaagg tcgtgcagga cgtgcgcctg agctgccccg gtgtcatgct ccatcggtca
     9241 cctcctcccg cgcccagtcc ggcgtgtagt agtcctcttc gtcgatcgtc aggatgtggt
     9301 acttggcctg ttccaggttc ctcaggtatc cccgccgcac cgcgatgacg aacccgaaca
     9361 gcgccgccag gaagaagacc aggaacgaga gcagcatcca gtcggccatc agccacgccg
     9421 cctgcccctg gtcgggggcg tcgtgagcga aggaggggcc ggccgtggcc agcacgacca
     9481 cccctgacag gagcaggacc gataggaccc gacggatcgt cttcatctca gctcacgctc
     9541 ctacggcgca tgccactgga ccccttccgt gctgaagatc tcgctcctgg gaggcatggg
     9601 ctcgccccgg cccagcgcat cctggtagtc ctgctggacg aactggcggt accgctcctc
     9661 caccatcttg gcgatcgtcg ccaggttcac cggaagcttc cccgcgatgt acaccgtgtc
     9721 ttggggagcc agccctggtg cgatccagcg cgccgcctgc tcgaacggac cggccgtcgg
     9781 gtcgtagccc gcgttgggat cggaaaccgg atggtccttg atgtagttga ggaggccctg
     9841 ctgcggctcc cacttgatga acatgtcggt ggtgggcagc gtttgcttca gacctccctt
     9901 ggcgatggtc tccaggaaga gcaccacccg ccaggtgtcc tcaaccgaca gccgcgtccc
     9961 gaagttctcc atgtcggtcc ccttcccccc ggtcagcacg cggtagtaga actgaccgtc
    10021 agaggcgtcc ggcgcggtca tctggtcgtc cacgtggatg aagtcggcgg gcttcggggc
    10081 catgaagcgc gcgcccgggc cgttgccgtc tccagtcggt ccgtggcaac cggaacaccg
    10141 cgtcaggtag atctgcttgc cgatcaccag gttgtcctcg gtcggtggaa gcgggttgcg
    10201 ggtcacccag taactgcgca ggtgtgtatt gacggcccag acgtcgcccc aggagaggcc
    10261 ccacttcgta gtgtcatcga tcgtgccgtc ggcatggtag gtaccggccg actgtgcttg
    10321 agacacctcg gtggggaaca cgttggccag gtctttgagg gttccctcga cgcccccgtt
    10381 ccagagcgcc ttgccgacaa ggatggcgga gttgcggagg gtggcctcct tcccgccctg
    10441 ggactggttg aaggcgatca tgtccttcag ctggctgtcc gagaagaagt tgaagcgcgg
    10501 catgatcgag atcggcgtgg tgttccgcgg gtcgtcgtag tgagctcgtt gccagtcgtc
    10561 cgggtgattg ccgccttcct gggacaggtc aggaccagtc cgctcgctcc ccaggatgtt
    10621 cgggctgtcg ttgcccgcgt agtcgcccgg ctcggagacc cgcgggtaga ggtaatagag
    10681 ccccaccgcc acgtcctgtg ggcggctgaa cccgctgtgg cagtagacgc agccgttcga
    10741 caggtacacg gctcgtccgc gcagcgccag gttcgagagc ggcagccagt tggtcgaggc
    10801 cggtggatga tacgtctcgg tgggaaggac caccaccatc agcaccaccg tgaagaaggc
    10861 cagcaacccg ccgatcgccg ccaggtgcgg ggtcatcagc aggcgacggc gtggcccctc
    10921 ccgcgtgtaa tcgatcgcgc tgcgcggctc ctggaactcg ccagggatgt gctcagccac
    10981 gggccgtcgc ctccgccggc tcgccggccc gcaggaaggg ctcgatctcg tctcgggtct
    11041 tctggtgagt gtcgccccgg accgttcgcc agatgttgta gatctgcacg atcccggaca
    11101 tcacgatcat cgcgccgaac acaccacgcg cgatgtagtg gggccggatc tcgggcagca
    11161 cgttcacctc ggggatcccc agggcccagt tctggccctg gaggaagctg gcgaaggtca
    11221 gggacacgaa gaagcccgtg aacccgatca ggatcagcca gtactgccac tccaccagcg
    11281 agcgactgta gatcggtcgc ttgtagatct gggggatcac gtagtcgatc acgcccatcc
    11341 ccaggatcgt gaacgctccc agaagcgcga gatgcgcgtg cgccacgatg aagttcgtga
    11401 agtggatcac ccggttgaac gagggcaggg ccatgaaggt gccctggatg ttgaccaggt
    11461 cgtagaagat gaacgcggtg atggtgaaac gtaagggtaa gttcgtgaag accttctccc
    11521 agttcccgcg catcgtgagc gtgatgttgg cgatgaacgc gaacacgggg atcagcagga
    11581 agatgctgtt gacggtggcg aaggtcttca gccacccggg gatcggcgcc tggagcaggt
    11641 ggtggtgccc cacccccgta tagaagaacg cgatacccca gaacgagatc agcgacagcg
    11701 tatggctgta caggggcgtg ttcgtgaccc gcggaacgat gtagtaggtg agggccacca
    11761 gcatcggcgt gagccacagg ccgaacaggt tgtggttctg ccaccagttc aacatcccat
    11821 cggccagcgg gttggtcagc gctccggaga tgtgaccgcc gggaacgccg ttgccgagca
    11881 tgctccccgg acgccacatc acgttgccga tgatgtagtc ggccaccaac cagagggggc
    11941 tgcccaggaa ccaccacacc gtcacgtaca ggggcttgat ccgacggttc aagaccgtca
    12001 tcacgatgtt gtagatgttc accagccaga tcgcgacgag caccacgtcg atcggccaga
    12061 tgaactcggc gtactcacga ccctgcgtgt aaccgagcgc aagggtgatg accccggctg
    12121 tgaacatggc gttccaggcc caggccatcc agatcgccgt gcgctcgctc cacatcgacc
    12181 ggaccccggt gagccgggga aggacgtaga agatcccgcc gaagtacatc gaggacagcc
    12241 aggcgaagat cacgccgttc acgtggatcg ggcggatgcg gctgaacagg agccatggga
    12301 taccgccgaa cagatcgggc gtcaccagct cggtggccat cgtcagccca accaggtcta
    12361 cgaaggtgag ccagaagatg gagctgaaca gccagatctt cgcggcccgg tcgtcgtact
    12421 cgctgacggg ctcgtcgacc tggagcactt ccggcgagac gaaccctatc cccctgccgt
    12481 tcctggtccc cgtcgtgacc gccatcgctc tccggtcctc cctccggtga tcggtgggac
    12541 gggcccgcct cacgcgacgc ccgacggcac cgacgctacg cgcgaccccc gccacaggtc
    12601 cgtcacggct ccgtaacttc tgtgtcacaa gcccttccgt ggggtcccca accggtcgga
    12661 ccggaggtac cgtggcccgg acggcagccg ccggcacggc ctgccatccg aggcggcgcg
    12721 ccggccggac aggcgtgggc ggagccgtgc ggttgcggag gacgagccgg gtgatgtcgg
    12781 ggtcgcggat ggagccgaag tgcgaggtgc cgatccaggt cgtcggcgcg gggtacccgc
    12841 ggacgtcgat ccggacgctc gagcgcctgg tccgggaagg atcggacgtc taccgccgtc
    12901 tgcggcacgc cgggatcgac gacgccgtgg tgctgtccac gtgccaacgg ctcgagatct
    12961 acggggcgtc cccccatgcc ccggcccacg ccgggctgcc gtccctcgaa cgcatcgtcg
    13021 aacccgcgac cgaaggaccg cccgcttccg acctcttcga actgtcggga tcggcggcgg
    13081 tggagcacct gttctccgtc gccgccggga tcgagtcgat cctgcccggg gaaccccaga
    13141 tcctcaccca ggtccgcgaa gcgctggagc gcggggaggc cgagcggatg gccgggcccg
    13201 ccctgcggcg gctgttccac gcggcccttc gcgccggccg acgggtccgc agggaggcgg
    13261 ggatcgggct ggcccacgac gccgtggtcc aagcagccat cgacctcgcg atggaccttc
    13321 ccggtcacgc gcggccccag gtcacggcgg tggtgggggc cggccgtatg gcccggatcg
    13381 ccgtgcagca cctcgcgcgc ctcggactca acgtccggac ggtcctcgac cggacaccgg
    13441 agcgggcctc cgatctggcg gccggcg
//
LOCUS       DASQQQ010000051         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_168829, whole genome shotgun sequence.
ACCESSION   DASQQQ010000051 DASQQQ010000000
VERSION     DASQQQ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168829"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VE646_02360"
     CDS             <1..183
                     /locus_tag="VE646_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HZD78870.1"
                     /translation="SALVAAVHTGHFPSFDEAARGMVRIAEVIEPRQEHRGVYDELYH
                     RYRETYPALRGLMHGG"
     gene            360..1955
                     /locus_tag="VE646_02365"
     CDS             360..1955
                     /locus_tag="VE646_02365"
                     /inference="COORDINATES: protein motif:HMM:NF033572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
                     /protein_id="HZD78871.1"
                     /translation="MTAAALERHTSDCTPGHPAVYFAAAHFFFRQLDNWLNSDEANHL
                     SEADAEAQVQCRGRELLRLLLQAHLAQRGTGQVGHALRVFDPPTPTQGPDDPARGPDA
                     TAQAPPPPPQPAARHAEKRLHGRTLHTTLGEVIVWRTGYAAPGRTSVHPLDQQLRLPG
                     RCFSHPLQEDLLRHAVQGPFGEAVGHVRRATGIALSKRSAEQLGVEAAADFQAFYRQQ
                     PAAEAGGGGPIVVTGIDCKGVPMIKPEKTLRKARLRKGDKKHKKRMATVASVRTAQPR
                     VRSPEEVADSLFAATPKDKPPRVRRKPPAPAEHKRVWADLLASKDAVIAEVVEEVRRV
                     DPSGAKRHVALCDGERALKRRILPALQKVAPGAVLILDLMHVLEKLWKVGHCFYEEGS
                     DEATAWVRQQALRVLRGEVGAVIEGIQVRAGEGGLKGSPRESVDKACRYLQRNREYMR
                     YDEYLRQGLPIASGCVEGACKHLIKDRLERSGMRWSLEGAQAMIRLRSLYVSGDFDEY
                     WDFHLQQEQRRLYPPGRWEVVEE"
     gene            1970..>2168
                     /locus_tag="VE646_02370"
     CDS             1970..>2168
                     /locus_tag="VE646_02370"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HZD78872.1"
                     /translation="MCILVLLYHVAEDAPVVAGANREEFYQRGGEPPRLLEGPGPGGL
                     RAVAGIDPVAGGTWFGVNSAGV"
BASE COUNT          351 a          750 c          782 g          285 t
ORIGIN      
        1 agcgccctgg tcgccgcggt gcacacgggc catttcccct cgttcgacga ggcggcccgg
       61 ggtatggtgc ggatcgccga ggtgatcgag ccgcggcagg aacatcgcgg agtttacgac
      121 gagctgtatc accgataccg ggagacgtac cccgccctgc gcggcctgat gcacggcggg
      181 tagaacggaa ttcattccgt tcgtgggact ggcggaacgg aatgaattgg gcgtggctac
      241 ttttcgtcgg gtagaaaagg tcgaggtgtc aaggggttgc ggcgtgaggg gcggacaagg
      301 caagcccccg ggacgaaaat aggggcgtta gccggcccta tcccccccag gagcttgcca
      361 tgaccgctgc tgccctcgaa cgccacacca gcgattgtac ccccggccac cccgccgtct
      421 acttcgccgc cgcccacttc ttcttccgac agctggacaa ctggctcaac tccgacgagg
      481 ccaaccacct cagcgaggcc gacgccgagg cccaggtcca atgccgcggc cgcgagctgc
      541 tccgcctgct cctacaggcc cacctcgccc agcgcggcac cggacaggtc ggacacgccc
      601 tccgcgtctt cgacccaccg acaccaaccc aagggcccga cgaccctgcc cgagggcccg
      661 atgccaccgc ccaggcccca cccccgccgc cccagcccgc cgcccgccac gccgaaaagc
      721 gcctgcacgg gcggaccctg cacaccaccc tgggcgaggt catcgtgtgg cggaccggct
      781 atgccgcccc cggccgcacc agcgtccacc ccctcgacca acagctgcgg ctgcccgggc
      841 gctgcttcag ccacccgctg caggaggacc tgctccgcca cgccgtgcag ggccccttcg
      901 gcgaggccgt cgggcacgtc cggcgcgcca ccggcatcgc cctgagcaag cgctccgccg
      961 agcaactcgg cgtggaggcg gccgccgact tccaggcctt ctaccgccag cagcccgccg
     1021 ccgaggcggg ggggggcggg ccgatcgtgg tgaccggcat cgactgcaag ggcgtgccca
     1081 tgatcaagcc ggagaagacc ttgcgcaagg cgcgcctgcg caagggggac aagaagcaca
     1141 agaagcgcat ggccacggtg gcctcggtgc ggacggcgca accccgcgtg cgcagccccg
     1201 aggaggtggc ggacagcctg ttcgccgcca cgcccaagga caagccgccg cgcgtgcgca
     1261 ggaagccgcc tgcgccggcg gagcacaagc gggtgtgggc ggacctgttg gccagcaagg
     1321 acgcggtgat cgccgaggtg gtcgaggagg tccggcgcgt ggacccgtcg ggtgccaaga
     1381 ggcacgtggc cttgtgcgac ggggagcggg ccctgaagag gcggatcctg ccggcgctac
     1441 agaaggtggc cccgggtgcg gtgctgatcc tggacctgat gcacgtgctg gagaagctgt
     1501 ggaaggtggg gcactgcttt tacgaggagg gcagcgacga ggcgacggcg tgggtgcggc
     1561 aacaggcctt gcgcgtgctg cgcggggagg tgggcgcggt gatcgagggg atccaggtcc
     1621 gcgccggcga gggcgggctg aagggctctc cgcgtgagtc ggtggacaag gcgtgccgct
     1681 acctgcagcg gaaccgggag tacatgcggt atgacgaata cctgcgccag gggctgccga
     1741 tcgccagcgg ctgcgtggag ggcgcgtgca agcacctgat caaggaccgc ctggagcgtt
     1801 cggggatgcg ctggagcctg gagggtgccc aggcgatgat aaggctgcgc tcgctatacg
     1861 tcagcggcga cttcgacgag tactgggact tccacctcca gcaggagcag cgacgtctct
     1921 accccccagg ccgctgggaa gtcgtcgaag agtagccacg ccctttgcca tgtgcatcct
     1981 ggtgttgctc tatcatgtcg ccgaggacgc ccccgtggtg gcgggggcca accgggagga
     2041 gttctaccag cgcggcgggg agccgccgcg cctcctcgaa gggcccggcc cgggcggact
     2101 gcgggccgtc gccggcatcg accccgtggc cggcgggacg tggttcggcg tcaactccgc
     2161 gggcgtcc
//
LOCUS       DASQQQ010000052         9305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_252391, whole genome shotgun sequence.
ACCESSION   DASQQQ010000052 DASQQQ010000000
VERSION     DASQQQ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9305
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252391"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VE646_02375"
     CDS             complement(<1..551)
                     /locus_tag="VE646_02375"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HZD78873.1"
                     /translation="MSESIYAVYPVYRYRGTPFDVDEAAQEVENLFKELSDQISVRGV
                     YSTVGFRADADLALWLVAPSPDAAQRAMVAFGRTQAGRVLAMTWSFMGVVKPAEFTAD
                     HAPAFVKGDPPKRFLNVYPFVRTSEWYLLPAEERAALLREHGEIGREFPDVLANTTTA
                     FGINDWEWILAFEADDLERIVDCL"
     gene            complement(548..1930)
                     /gene="hemG"
                     /locus_tag="VE646_02380"
     CDS             complement(548..1930)
                     /gene="hemG"
                     /locus_tag="VE646_02380"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HZD78874.1"
                     /translation="MSGRVAVAGAGIAGLTAAHRLTRRAPDLEVTVLESQAAPGGKIR
                     SARVGGVMLEAGPDSLLARKPAGVELCRELGLGDDLVAPGAGVAQIWTDAGLLRFPSG
                     PFGISTDLGELWRWEGMSRRGKLRAAGDLVRRARTDPSDESLGSLIRRRMGDEAAEKL
                     VAPLLGGLFAGDVDRLSVLATFPELARWERERGSLIRGARAAARAARGPRVAPMFLRL
                     RGGLIRLVEALGAELGPRLRCGVEVTGLSLEGDRYWVATSEGDLEADAVILATPAFVT
                     ADLVEPLAPAAAPELRAIPHVSTCVVLFVYEQGTGPALPESSGFVSPRGALAISAATL
                     VSRKWPDRSFGDRAVIRCFVGGVGTEELVEAPDEDLVEGVCRQLQAIYRLPERAAAAA
                     VVRWPRAMPQYEVGHLERVDRIERALPPRVALVGQGYRGTGIPDCIRGGDEAATRVAS
                     LLEVEGERVR"
     gene            complement(1927..2907)
                     /gene="hemH"
                     /locus_tag="VE646_02385"
     CDS             complement(1927..2907)
                     /gene="hemH"
                     /locus_tag="VE646_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006446402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HZD78875.1"
                     /translation="MSDPPIGVLVMAYGTASGPDDIERYYTDIRGGRPPTPEHLQELR
                     ERYAAIGNRFPLLEITRRQAEGLERDLNRPADGRTFRTYLGMKHSEPFIPEGVARMRA
                     DGIERALGIVMAPHWSGMSIETYVERVEKAVAEDGGPAFGFVRGFHDHPGFVDLLASR
                     IEQASKELPAEARDGAAVIFSAHSLPVRTLEDGSQRCKACFRCEASCRYRDGLRETAA
                     LVAERLGLEHATTGWQSAGRTADPWWGPPIEDLIRDVARQGRPGVVVCSAGFVADHLE
                     ILYDLDIEAREVAEDAGIAFARTRMPNADPAFTALLAEVVRAPPQAAVPS"
     gene            complement(2904..3947)
                     /gene="hemE"
                     /locus_tag="VE646_02390"
     CDS             complement(2904..3947)
                     /gene="hemE"
                     /locus_tag="VE646_02390"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HZD78876.1"
                     /translation="MAPAPLPRFIAACRRQPVDRTPVWFMRQAGRYLPEYGQARGSGD
                     ILQAVRTPELAVELTLQPLRRMELDAAIVFSDIMVPLAAIGVGVQIRSGVGPVVAEPF
                     RSAADLPRLRPLEPEADVPHVLETLRLLRKELEVPLIGFAGAPFTLASYLIEGGPSRD
                     QARAKALLHQEPTVWDRLMRSLGQIVLEHLRAQVAAGAQAVQVFDSWAGTLPPDVYER
                     AVLPTMRGIFGGLEDLGVPRIHFGVGTGELLALMRSAGADVVGVDWRVPLDAAWGRVG
                     HDLAVQGNLDPAVCLARWEVVRDEALTVLTRAAGRAGHVFNLGHGVLPGTPVGNLQRL
                     VDLVHRTTEREAT"
     gene            complement(4039..6612)
                     /locus_tag="VE646_02395"
     CDS             complement(4039..6612)
                     /locus_tag="VE646_02395"
                     /EC_number="7.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012823845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasma-membrane proton-efflux P-type ATPase"
                     /protein_id="HZD78877.1"
                     /translation="MGGTHPEAERGAGSDEPGGPLISEGAGGASFLDAETAEKLPAEE
                     VFEALGSSSGGLTGDQAGRRLADVGPNALEEHRRSAVLQFLSYFWGPIPWMIEVAAIL
                     STIVRHWPDLIIILVLLVFNAGVGFWQEYQAASAVDALKRQLALKARVRRDGRWSQID
                     AADLVPGDVIRLRLGDVVPADVKLVEGDYLSVDQSALTGESLPVNKRPGDIAYSSSAA
                     KQGEMVAVVVATGARTYFGRTARLVSTAGAASHFQRAVLTIGDYLIYLSLFLVAILIF
                     VQLLRGNSFLTLVQFALILTVAAIPVAMPAVLSVTMAVGATVLSRMKAIVSRLESIEE
                     MAGMDILCSDKTGTLTQNRLTLGTPALAQARDAQEVILAGALASKEEDRDAIDLAVLA
                     GLDDPSATAGFTQTRFVPFDPVSKRTEADVRDRNGSVMRVTKGAPQVILDLCRLDRSE
                     ETGIQRQVEDFAHKGYRTLGVARRDDGDWRFLGLLPLFDPPREDSADTISRAEEHGIQ
                     VKMVTGDDMAIAKEIAGELDLGTNILPAAQVFEGDEARWDRPTVREAIEGADGFARVF
                     PEHKFNIVKTLQSGEHIVGMTGDGVNDAPALKQADVGVAVSGATDAARAAADLVLTAP
                     GLSVIVRAVEEARRIFERMNSYAIYRITETIRIMFFVVFTMIAFNFYPITAILIILLA
                     LLNDLPIMTIAWDRTWLDPEPVRWDMRRVLTISTVLGLTGVVSSMLILAWLRVGLKLG
                     ISEIQTLIFLKLAVAGHLTLFIARTREPFWSKPYPAPIMLWSAVGTKVLATLVAAFGF
                     GLITPVPWWAIGLVWGYAILWMVILDLVKVATYRHLELSGGHHRRFLGRLRATLHPFP
                     W"
     gene            complement(6941..7771)
                     /locus_tag="VE646_02400"
     CDS             complement(6941..7771)
                     /locus_tag="VE646_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78878.1"
                     /translation="MPRRRSEIDELFGLEPGRFVAARDELVKRLREGGQEARATQVKA
                     LRRPTVAAWTLNQLARQHREELRGLLEAGEELRRAQRTILSGGSADLTGAGERRRALI
                     AELMRWAGELLAAQRRDSAATREAIREGLEAASADEQTARAALEGHLSKGPSAPVGFG
                     GIDGLQVVPGGRATDGGDASPATDPDSLGAKARDLDQVVRRAEREVDKAQRGAARAED
                     AARRLRREADQADRRAAEARALAERAARDLASARSDRDAAAAARTAARSRGRPTSPRG
                     "
     gene            8124..9059
                     /locus_tag="VE646_02405"
     CDS             8124..9059
                     /locus_tag="VE646_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019900804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HZD78879.1"
                     /translation="MAWTTDDIPDQTGRIAVVTGANGGIGFEVARELARKGARVIMAC
                     RDMERAGSARRAIIEETPGASLEVQEIDLASLGSVRAAAGRILAAHPRIDLLVNNAGV
                     MGIPERRTVDGFEMQLAVNHLGHFALTALLLPALLAAPAARVVSVTSNGRIIGRPLGH
                     ENPHLVDRYGPWRAYGQSKLAAVHFALELDRRFRRAGVAARSLVVHPGFTHTDLQARS
                     ARETGDLLGRLSYAAVRRVGMSPARGALSLLRAATDPNVDGGTLYTPRWVNSGPPVRR
                     PLFARSRDPAAMRTLWEVSERETGVRLDLPTAHDA"
BASE COUNT         1380 a         3500 c         3126 g         1299 t
ORIGIN      
        1 aggcagtcca cgatccgctc caggtcgtct gcctcgaagg ccaggatcca ttcccagtcg
       61 ttgatcccga aggcggtggt cgtgttggcc agaacgtccg ggaactcgcg cccgatctcg
      121 ccgtgctcgc gcagcagggc ggcccgctcc tctgccggca ggaggtacca ctcggaggtg
      181 cggacgaacg gatacacgtt caggaaccgc ttcgggggat cgcccttgac gaaggccggg
      241 gcgtggtcgg ccgtgaactc ggcgggcttg accacgccca tgaacgacca ggtcatcgcc
      301 aaaacccgcc ccgcctgcgt ccgcccgaac gccaccatcg cccgctgcgc cgcatccggc
      361 gagggagcca ccagccacag ggcgaggtcg gcgtcggccc ggaagccgac cgtcgagtac
      421 acgccccgga ccgagatctg atccgacagc tccttgaaga ggttctcgac ctcctgcgcc
      481 gcctcgtcca cgtcgaaggg cgtcccccgg taccggtaga ccggatacac cgcgtagatc
      541 gactcactca tcgcacccgc tccccttcca cctcgagcag cgacgccacc cgcgtcgccg
      601 cctcgtcccc gccccggatg cagtccggga tgcccgttcc tcgatacccc tgccccacca
      661 gcgctacgcg cggtgggagg gcccgctcga tcctgtccac ccgctccagg tgccccacct
      721 cgtactgcgg catcgctcgc ggccaccgga ccaccgccgc ggcggcggcc cgctccggga
      781 ggcggtagat cgcctgcagc tgacggcaca ccccctccac gaggtcctcg tcaggcgcct
      841 ccaccagctc ctcggttccc acccccccga cgaagcagcg gatcaccgcg cggtcgccga
      901 acgagcgatc cggccacttg cgcgacacga gcgtggcggc gctgatcgcc aacgcccccc
      961 tcggggacac gaagccggag gactcgggga gcgcggggcc ggtgccctgc tcgtagacga
     1021 acaggaccac gcaggtcgac acgtgaggga tggcgcggag ctcgggggcg gccgcggggg
     1081 ccagcggctc caccaggtcg gccgtgacga acgcgggggt cgccaggatc accgcatcgg
     1141 cctccaggtc tccctcggag gtggccaccc agtatcggtc gccctccagg gacaggccgg
     1201 tgacctcgac gccgcaccgg agccggggcc cgagctcggc gccgagcgcc tccaccagcc
     1261 ggatcagccc accccgcagg cgcaggaaca tcggcgccac gcggggtccg cgggcggccc
     1321 gggcggcggc ccgagccccc cggatcaggc tgccccgttc ccgctcccag cgagccagct
     1381 cggggaaggt ggccagcacg ctcagccggt ccacgtcgcc cgcgaacagc ccgccgagga
     1441 gcggagccac cagcttctcg gctgcctcgt cgcccatccg ccgccggatc aagctgccca
     1501 acgactcgtc ggaggggtcg gtccgcgctc ggcgcaccag atccccggcc gcccggagct
     1561 tgccccggcg cgacatcccc tcccatcgcc acagctcgcc caggtcggtg gagatgccga
     1621 agggcccgct cgggaagcgc aacagccccg cgtcggtcca gatctgcgcc acacctgctc
     1681 cgggcgcgac caggtcgtca cccaggccga gctcccggca caactcgacc ccggccggct
     1741 tgcgggccag cagggagtcc ggacccgcct ccagcatgac accacccacc ctcgcggagc
     1801 ggatcttgcc cccgggtgct gcttgggact cgagcaccgt gacctccagg tccggcgccc
     1861 gtcgggtcag gcggtgggcc gcggtcagac cggcgatgcc cgcgcccgcg accgccaccc
     1921 gaccgctcac gagggcaccg ccgcctgcgg gggggcccgc acgacctcgg ccaggagggc
     1981 ggtgaaggcc gggtcggcgt tcggcatccg ggtccgcgcg aacgcgatgc ccgcgtcctc
     2041 ggccacctcg cgcgcctcga tgtccaggtc gtagaggatc tccaggtggt cggccacgaa
     2101 gcccgcagag cagaccacca ccccgggccg tccctgcctg gccacatcgc ggatcaggtc
     2161 ctcgatcggg ggaccccacc aggggtcggc ggtacggccg gcgctctgcc agccggtggt
     2221 cgcgtgctcc aggcccaggc gctcggcgac cagggccgcc gtctcccgga gcccgtcacg
     2281 gtaccggcag gacgcctcgc accggaagca cgccttgcag cgctgggagc catcctccag
     2341 cgtccgcacg ggcaggctgt gcgccgagaa gatcaccgcg gcgccgtccc gggcttcggc
     2401 gggaagctcc ttgctggcct gctcgatgcg ggaggcgagc agatccacga acccggggtg
     2461 gtcgtggaag ccgcggacga agccgaacgc cgggcccccg tcctcggcca cggccttctc
     2521 cacccgttcc acgtaggtct cgatggacat ccccgaccag tgcggcgcca tcacgatccc
     2581 cagcgcgcgc tcgatcccgt cggcgcgcat ccgggccacc ccctcgggga tgaagggctc
     2641 cgagtgcttc atgcccaggt acgtgcggaa cgtccggcca tcggcgggac ggttcaggtc
     2701 ccgctccagg ccctcggcct gccggcgcgt gatctccagc agcgggaagc ggttgccgat
     2761 ggccgcgtac cgctcgcgca gctcctggag gtgctcgggg gtgggcggac gtccaccgcg
     2821 gatatccgtg tagtaccgct cgatgtcatc gggaccgctg gccgtcccgt aggccatgac
     2881 caggaccccg atgggcgggt cactcatgtc gcctcccgtt ccgtggtccg gtgcacgagg
     2941 tccaccaggc gctgcaggtt ccccaccggc gtgccgggga ggaccccgtg cccgaggttg
     3001 aagacgtggc cggcccgtcc ggccgcccgg gtcagcaccg tcagtgcctc gtcccgcacg
     3061 acctcccatc gggccaggca gacggccggg tccaggttgc cctggacggc gaggtcgtga
     3121 ccgacccggc cccaggccgc atccaacggc acccgccagt cgacgccgac cacgtccgct
     3181 ccggccgagc gcatcagcgc cagcagctct ccggtcccca ccccgaagtg gatgcgggga
     3241 acgcccagat cctccagacc cccgaagatc cctcgcatcg tgggcaggac cgcccgctcg
     3301 tacacgtccg gcggcagcgt gcccgcccag ctgtcgaaga cctgcaccgc ctgagctccg
     3361 gcggccacct gcgcccggag gtgctccagg acgatctggc ccaggctccg catcagccgg
     3421 tcccatactg tcggctcctg atggagcagc gccttggcgc gagcctggtc ccgggacggg
     3481 ccgccctcga tcaggtagct ggccagcgtg aagggtgcac cggcgaaccc gatcaggggg
     3541 acctccagct ccttgcggag gaggcggagc gtctccagga cgtggggcac gtcggcctcc
     3601 ggttcgagcg gccggaggcg gggaaggtcc gccgccgaac ggaacggctc cgccacgacg
     3661 ggccccaccc cggaccggat ctgcaccccg accccgatgg cggccaacgg caccatgatg
     3721 tcggagaaga cgatcgcggc atccagctcc atccggcgga gcggctggag ggtgagctcg
     3781 acggccaact ccggcgtgcg gacggcctgg aggatgtcac cgctcccccg tgcctgtccg
     3841 tactcgggca ggtaccggcc ggcctgtcgc ataaaccaca cgggggtgcg atccaccggt
     3901 tgtcgccgac acgcggcgat gaaccggggg aggggagccg gagccatggc acacaggata
     3961 cgtaccggtg ccccgctcgg cctcggccgg gcggggttcg aacgttcctc gtgatcgatc
     4021 gccgcccctg cccccgcctc accaggggaa ggggtggagc gtggccctca gccgtcccag
     4081 gaacctgcgg tgatggcccc cggagagctc gaggtgccgg taggtcgcca ccttgaccag
     4141 gtccaggatc accatccaca ggatcgcgta tccccacacg agcccgatcg cccaccacgg
     4201 caccggtgtg atcaggccga agccgaaggc cgccaccagc gtcgccagga ccttcgtgcc
     4261 gacggccgac cacagcatga tcggggccgg gtacggcttc gaccagaagg gctcgcgggt
     4321 ccgtgcgatg aagagcgtga ggtggcccgc cacggccagc ttcaggaaga tgagcgtctg
     4381 gatctcggag atccccagct tcaggccgac ccggagccac gcgaggatca gcatgctgga
     4441 caccacgccc gtgaggccga gcaccgtgga gatcgtgagc acccgtcgca tgtcccaccg
     4501 gaccggctcc gggtccagcc aggtccgatc ccaggcgatc gtcatgatcg gcaggtcgtt
     4561 cagcagcgcc agcaggatga tcaggatcgc ggtgatggga tagaagttga acgcgatcat
     4621 cgtgaagacg acgaagaaca tgatccggat ggtctccgtg atccggtaga tcgcgtagct
     4681 gttcatccgc tcgaagatgc gccgcgcctc ctccaccgcg cggacgatca ccgagagccc
     4741 cggggcggtc aggaccagat cggccgcggc tcgggccgca tcggtggctc cgctgacggc
     4801 gacccccaca tccgcctgct tcagggccgg cgcgtcgttc accccgtcgc cggtcatccc
     4861 gacgatgtgc tcgcccgact gaagggtctt cacgatgttg aacttgtgct ccgggaacac
     4921 ccgcgcgaac ccatcggccc cctcgatggc ctcacgaacc gtgggccggt cccaccgggc
     4981 ctcgtctccc tcgaacacct gcgccgccgg caggatgttc gttccgaggt cgagctcccc
     5041 ggcgatctcc ttggcgatcg ccatgtcgtc gccggtcacc atcttcacct ggatcccgtg
     5101 ctcctcggct cgggagatgg tgtccgcgga gtcctcccgg ggcgggtcga acagcgggag
     5161 gagacccagg aaccgccaat cgccgtcgtc gcgtcgggcc acccccagcg ttcggtaccc
     5221 tttgtgcgcg aagtcctcga cctgtcgttg gatcccggtc tcctccgagc ggtcgagccg
     5281 acacaggtcc aggatcacct gaggcgctcc cttggtgacg cgcatgacgc tgccgttccg
     5341 atcccggacg tcggcctccg tccgcttgct caccgggtcg aaggggacga agcgggtctg
     5401 ggtgaacccg gccgttgccg agggatcgtc tagcccggcc aggaccgcca ggtcgatcgc
     5461 gtcccggtcc tcctccttcg aggccaacgc gccggccagg atcacctcct gggcatcgcg
     5521 cgcctgcgcc aacgccggcg tcccgagggt cagtcggttc tgggtcaggg tccccgtctt
     5581 gtccgagcag aggatgtcca tccccgccat ctcctcgatc gactcgagcc tggagacgat
     5641 ggccttcatc ctcgacagga ccgtcgcccc caccgccatc gtgaccgaga ggaccgccgg
     5701 catggccacg gggatggcgg ccacggtgag gatcagggcg aactggacca gggtgaggaa
     5761 cgagttgccg cgcaacagct ggacgaagat gaggatggcg accaagaaca ggctcaggta
     5821 gatcaggtaa tccccgatgg tgaggaccgc acgctggaag tgcgatgccg ctcccgccgt
     5881 ggagaccagc cgggccgtcc gcccgaagta cgtcctggcg cccgtggcca ccaccacggc
     5941 gaccatctcc ccctgcttgg ccgccgagct cgagtacgcg atgtcgccgg gccgcttgtt
     6001 gaccggcagc gactcgccgg tcagcgccga ctggtcgacg ctcaggtagt cgccctcgac
     6061 gagcttcacg tccgcgggaa cgacgtctcc cagtcggagc ctgatcacat cgcccggcac
     6121 caggtcggcc gcgtcgatct ggctccagcg tccgtccctc cgcacccgtg ccttcagggc
     6181 gagctggcgc ttcagagcat ccaccgcgct ggccgcctgg tattcctgcc agaacccgac
     6241 gccggcgttg aagaccagca ggaccaggat gatgatcagg tccggccagt ggcgcacgat
     6301 ggtcgaaagg atcgccgcga cctcgatcat ccaggggatc ggaccccaga agtaggagag
     6361 gaactgtagg accgcgcttc tgcggtgttc ctccagggcg ttgggcccca catcggccag
     6421 ccgtcgaccg gcctggtcgc ccgtcagccc gccggaactc gatccgagag cctcgaagac
     6481 ctcttccgcc gggagcttct ccgcggtctc cgcatcgagg aacgatgccc ccccggcccc
     6541 ctccgagatg agcgggccgc ccggttcgtc cgatccggct ccccgctccg cttcgggatg
     6601 ggttccgccc atcggcacgc ctcctctcgg cacggtcgcg gctccctaca cgctagtacg
     6661 cgcgtcggag aacggaccca ccgggaggag gagcacggtc gggacgagaa aacgcgcggg
     6721 ctccagagag cgggaggggt gggcatcctc gtggaggttg acgtcgagcg cctcccacac
     6781 gtgctccgga acgatccggc gatcgcgggt ctcagatgcc cttggccgct ggtggtcctg
     6841 aggacgcgag aagacctgga ggctctgccc ttcgacacct cgtcccaggt ctggaagacg
     6901 atcgggaggt acgcaaaggt ccccgcgtcc cagtagatgg tcacccacga ggggacgtcg
     6961 gcctcccccg gctccttgcc gccgtccggg ctgcagcggc ggcatcccgg tcgcttctcg
     7021 cacttgcgag gtccctagcg gcccgttcgg ccagggcccg agcctcggcc gcccgacgat
     7081 ccgcttgatc cgcctcccgc cgcaaccggc gtgccgcgtc ctcggcgcgc gccgccccgc
     7141 gctgggcctt gtccacctcc ctctcggccc ggcgcacgac ctgatccaga tcgcgggcct
     7201 tggccccgag gctgtcagga tccgttgcgg gagaggcatc ccccccgtcc gtggcccggc
     7261 cccccgggac gacctggagc ccgtcgatcc ctccgaaccc caccggggcg gagggcccct
     7321 tggacaggtg accctccagc gccgcccgtg cggtctgctc atcggccgag gcggcctcca
     7381 ggccctccct gatcgcctcc cgggtcgcgg cggagtcccg tctctgcgct gccagcagct
     7441 cacctgccca ccgcatcagc tcggcgatca gcgcccgacg cctctctcca gctccggtca
     7501 ggtcggcgct gcctcccgac aggatcgtgc gctgggcccg tcggagctcc tcccccgcct
     7561 ccaggagccc ccggagttcc tcgcggtgtt ggcgggcgag ctggttcagg gtccaggccg
     7621 caaccgtcgg acggcgcagg gccttgacct gggtcgcccg tgcctcttga ccgccctccc
     7681 ggagccgctt gaccaactca tcacgcgccg ccacgaaccg acctggttcg agcccgaaga
     7741 gctcgtcgat ctcggatctg cgccttggca tcagatcggc cggcccggat ggctcgcgtc
     7801 tcccaagggc tcaagtatgc ctgggtgggg cggttcgagc gaaccgcggt ccggggtccg
     7861 gcctcacgcc gaccctgtct ccgccggtcg aggtgagcgg ccccttcggc cgccgggcgc
     7921 cagttggtga gggggccgct ggctcggcga cgatgcgtcg gcgccagtga gctccgggtc
     7981 cgaccctcag ggcccacgcc accgggatct tcggtggcgt caccgtgaag aacaagaccg
     8041 accgaccgac cggccggttc cggccacccc ctgctgcggt cgctgttcag ctgagcctat
     8101 cctggaccga gcgaaggatc cgcatggcct ggaccacgga cgacatcccc gaccagactg
     8161 gccgaatcgc ggtcgtcacc ggcgctaacg gcggcatcgg cttcgaggtg gcccgcgagc
     8221 tcgcgaggaa gggcgcgcgc gtcatcatgg cttgtcgcga catggagcgg gccgggtcgg
     8281 cacgacgggc gatcatcgag gagacccccg gcgcgtccct cgaggtgcag gagatcgacc
     8341 tcgcttcgct cgggtcggtc cgggcagctg ccgggcgcat cctggcggcc catcccagga
     8401 tcgacctctt ggtgaacaac gccggcgtga tgggcatccc cgagcgacgg accgtggacg
     8461 ggttcgagat gcagctcgcc gtgaaccacc tgggacactt cgcgctcacc gcgctgctgc
     8521 tgccggccct gctcgcggcc cccgccgctc gcgtggtgtc ggtgacgagc aacggccgta
     8581 tcatcggccg cccgctgggc cacgagaacc cccatctggt ggaccggtat ggcccgtggc
     8641 gggcgtacgg tcagtccaag ctcgccgccg tgcacttcgc gctggagctc gaccgacggt
     8701 tcaggcgggc gggcgtcgca gcgaggagcc tggtcgtcca tccggggttc acgcacacgg
     8761 acctgcaggc caggagcgcg cgggagaccg gtgacctgct cgggcggctc tcgtacgcgg
     8821 cggtccgccg ggtcgggatg tcgcccgccc gtggggcgct gtccctgctt cgtgccgcga
     8881 ccgacccgaa cgtcgacggc gggacgctct acacgccgcg gtgggtcaac tcaggacctc
     8941 cggtccggcg cccgctgttc gcccgctcca gggatccagc ggcgatgcgc accctatggg
     9001 aagtctccga gcgcgagacc ggggtgcggc tggacctccc aacggcccac gacgcatagg
     9061 gttgatgtcg ccggagacaa ctccacttac gggttggggc cttcgacacc aggaggacgg
     9121 gcggccttgc gcctcgaacc tctcaaccgc cgccttcccc gaaacccgcc ggccgtcgaa
     9181 gaggggacca ccgcccggcc tccaggacgt cggtggcacc gcgacgcccc cgctcggcgg
     9241 cctcatcacg agtacgagcc ggctgcatga acgagtcgga tcgggttgcc ggcacccaca
     9301 cgtgg
//
LOCUS       DASQQQ010000053         3289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_279138, whole genome shotgun sequence.
ACCESSION   DASQQQ010000053 DASQQQ010000000
VERSION     DASQQQ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279138"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VE646_02410"
     CDS             <1..386
                     /locus_tag="VE646_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ribulose phosphate epimerase"
                     /protein_id="HZD78880.1"
                     /translation="IELLPRFGFTYSSNLMDDVRPYRHDRSDVIELPVHWVLDDAPHF
                     WFDAGSWTKTIRTAAEVEALWEEEVAGIRALGGLAVVTMHPQVIGRPGRLAMLDRFLG
                     WVRRLGDAWIAPCAEIAAHAREADR"
     gene            383..1567
                     /locus_tag="VE646_02415"
     CDS             383..1567
                     /locus_tag="VE646_02415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZD78881.1"
                     /translation="MTGAGASGGELAVEVAVVGGGVIGLSVARELCRAGVHRVVVLEA
                     LTSVGRGSSTRANGGFRAQFTTRPNIEFSRYSIGELERLEAATTLLSLHQVGYLLITG
                     TEDGERGLRDARELQRSLGVPTEWLEPKQVLERAPFLRAEGLRAGTFHARDGFLDPHG
                     LVGALFGEARRLGSQVLTGSPVTAIHRAPTGDLVLEHPGGGVRASWVVNAAGADAAAV
                     AGMLDLDLPVTPVRRSLAYAREAGVAPAELIPMCVDLDTGVLVRREPAGGYVIAYSDP
                     DDPPSRETTVDPAFLDQVARRIGNRFPHLAEVPIDPDRCWAGLYPETPDHHAIIGEAP
                     DMPGFVQCAGFGGHGLMHAPAAGRAVAELVTRGRCETFDLHPLRPSRFAEGDLVVETA
                     VL"
     gene            complement(1623..2558)
                     /locus_tag="VE646_02420"
     CDS             complement(1623..2558)
                     /locus_tag="VE646_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZD78882.1"
                     /translation="MSASRSARPHALGVQGSRMGAVAVLSAVSIWGVTNTLVKLSHLS
                     ALSFAVYRLGLGAVVLFGALLVSRRRLTLPQIRAALPAGVLFGVQIVFWFGALKHTSL
                     VDATMISALQPALVLLVAGRMFGEHAARRDVLLTLASLVGVAVVTFGSAGTPAWSLGG
                     DLLAVASLLGWTAYMLLSKRVRDRVPPLQYMAVVFLIAAVLVLPIALVAGAPLGGVRT
                     TDWLILLVFVIGASSGHVLLAWAHSSVDVSLSSTLLLAQPVLTAVAALLILGEPIPVL
                     AVVGGAVVLGSLGAVVRHASIAGPPPELESPDVPG"
     gene            complement(2595..>3289)
                     /locus_tag="VE646_02425"
     CDS             complement(2595..>3289)
                     /locus_tag="VE646_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZD78883.1"
                     /translation="QIHRPERETDVEETLGALTDLQRAGKIRYFGSSTFPGWQIVEAQ
                     WAAERRGLSRFRTEQPPYSIFVRQIEHDVLPVAQRYGMGVLVWSPLNRGWLTGRYRRE
                     GFDRAPGTRAARSTEGGGPLAKQFDESRPEIQRKLDLVERLLGIASEAGIPLTHLAIA
                     FTLAHPAVTSAILGPRTMDQLADLMAGADVHLDGDTLDAIDQLVPPGTLVDENDRGFG
                     PWWFEPSERRRG"
BASE COUNT          478 a         1156 c         1204 g          451 t
ORIGIN      
        1 cgatcgagct cctgcctcgc ttcggcttca cgtactcgag caacctcatg gacgacgtac
       61 gcccgtaccg gcacgatcgg tccgacgtga tcgagctccc ggtgcactgg gtgctggacg
      121 acgctcccca cttctggttc gacgcgggat cgtggacgaa gacgatccgg accgccgcag
      181 aggtggaggc cctgtgggag gaggaggtgg ccgggatccg agcgctcggc ggtctggccg
      241 tggtcacgat gcacccccag gtgatcggcc gccccggtcg tctggccatg ctggaccggt
      301 tcctgggatg ggttcggcgg ttgggcgatg cgtggatcgc tccctgcgcc gagatcgcgg
      361 ctcacgctcg ggaagccgac cgatgaccgg agccggggct tccggcggcg agctcgcggt
      421 cgaggtggcc gtggtggggg gcggtgtgat cgggctgtcg gtggcccgag agctgtgccg
      481 ggcgggggtg caccgggtgg tggtcctgga ggcgctgacc tcggtggggc gcgggtcctc
      541 cacgcgcgcg aacggcggct tccgcgcgca gttcacgacc aggccgaaca tcgagttctc
      601 ccggtattcg atcggcgagc tcgaacggct cgaggccgcg accaccctgc tgtcgctgca
      661 ccaggtgggg tacctgttga tcaccgggac cgaggacggg gagcggggcc tgcgggacgc
      721 ccgcgagctc cagcgctccc tgggcgtacc cacagagtgg ctggagccga agcaggtgct
      781 ggaacgggct cccttcctgc gagcggaggg ccttcgagcc ggcaccttcc acgcccggga
      841 cgggttcctg gatccccacg gcctggtcgg cgccctgttc ggggaggctc ggcggctggg
      901 ctcccaggtc ctgaccgggt ctccggtcac cgcgatccat cgagcgccca ccggtgacct
      961 cgtcctggag catcccgggg gtggggtccg agcctcgtgg gtggtgaacg cggccggggc
     1021 cgatgcggcc gcggtggccg ggatgctgga cctggatctt ccggtcacgc cggtccgccg
     1081 cagcctcgcc tacgcgcggg aggcgggcgt cgctccggcc gagctcatcc cgatgtgcgt
     1141 ggacctggac accggcgtcc tggtccggcg ggagccggcc ggcgggtacg tgatcgcgta
     1201 ctcagatccg gacgacccgc cctctcgcga gaccaccgtc gatccggcgt tcctcgacca
     1261 ggtggcccgg cgcatcggga accggttccc ccacctcgcc gaggtgccca tcgaccccga
     1321 tcggtgctgg gcgggcctgt acccggaaac gcccgaccac cacgcgatca tcggcgaggc
     1381 gcccgacatg ccggggttcg tccagtgcgc cggcttcggg ggccacgggc tgatgcacgc
     1441 gccggcggcc ggtcgggccg tggcggagct cgtcacgcgg gggcggtgcg agaccttcga
     1501 cctgcatccc ctgcgtccct cgcggttcgc tgagggcgac ctggtggtgg agaccgccgt
     1561 cctgtagcgg tctgcgttgc ggcctggtcg ggagccggca cggtgtcccg gctcagcgtg
     1621 ccctatccag ggacgtctgg cgactcgagc tccggcggag ggccggcgat cgaggcgtgc
     1681 cgcaccacgg ctccgagcga ccccagcacc accgcgcctc ccaccaccgc cagcacaggg
     1741 atcggttccc ccaggatcag cagggctgcc acggcggtga gcaccggctg ggccaacagc
     1801 agcgtagagg acagcgacac gtccacggag gagtgggccc aggccagcag gacgtgaccg
     1861 gacgaggcgc cgatcacgaa caccagcagg atcagccagt cggtcgtccg gaccccgccc
     1921 agcggtgccc cggccaccag ggcgatgggg aggaccagaa cggccgcgat caggaagacc
     1981 accgccatgt actgcagcgg tgggacccgg tcccgaacgc gcttggagag cagcatgtag
     2041 gcggtccacc ccagcaggga cgccacggcg agcaggtccc cgcccaggct ccaggcgggc
     2101 gtccctgcgc tgccgaacgt caccaccgcc acccccacca gcgaggccag ggtgagcagg
     2161 acgtcgcgtc gggcggcgtg ctcaccgaac atcctgccgg ccaccagtag taccagggcc
     2221 ggctggagcg ccgagatcat cgtggcgtcc acgaggctgg tgtgcttcag cgcgccgaac
     2281 cagaagacga tctgcacccc gaagagcacc ccggccggca gggcggcccg gatctggggc
     2341 agggtcaacc gacgcctgga cacgagcagc gcgccgaaca gcaccaccgc gcccagcccc
     2401 aaccggtaga cggcgaagct gagcgccgac aggtgcgaca gcttgaccag cgtgttggtc
     2461 acgccccaga tcgagaccgc cgagagcacg gcgacggcgc ccatgcgcga tccctggacg
     2521 cccagggcgt gcgggcgagc gctgcgggag gcggacatcc cttccatctt cccagcaccg
     2581 ccgagggacg cggctcaccc ccgccgtcgt tcggatggtt cgaaccacca gggcccgaac
     2641 ccccggtcgt tctcgtccac cagcgttccg ggcggcacca gctggtcgat cgcatccagc
     2701 gtgtcaccgt ccaggtgcac gtcggcgccg gccatcagat cggcgagctg atccatggtg
     2761 cggggaccga ggatcgccga ggtcaccgcc gggtgcgcga gcgtgaacgc gatcgccagg
     2821 tgcgtgaggg ggatgcccgc ctcagaggcg atgcccaaca gccgttccac caggtcgagc
     2881 ttgcgctgga tctcgggccg cgactcgtcg aactgcttgg ccagtggtcc accgccttcg
     2941 gtgctgcggg cggcacgggt gccgggagcg cgatcgaagc cctctcgccg gtagcgcccg
     3001 gtgagccagc cccggttcag tgggctccag accaggacgc ccatgccgta ccgctgggcc
     3061 accgggagca cgtcgtgctc gatctgccgg acgaagatcg agtagggggg ctgctcggtc
     3121 cggaagcggg acaggccccg tcgctcggcc gcccactgcg cctcgacgat ctgccaaccg
     3181 gggaaggtgg aggagccgaa gtaccggatc ttccccgccc gctgcagatc ggtgagggcc
     3241 ccgagggtct cttccacgtc ggtctcgcgc tccggccgat ggatctggt
//
LOCUS       DASQQQ010000054         4789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_31655, whole genome shotgun sequence.
ACCESSION   DASQQQ010000054 DASQQQ010000000
VERSION     DASQQQ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31655"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1697)
                     /gene="aspS"
                     /locus_tag="VE646_02430"
     CDS             complement(<1..1697)
                     /gene="aspS"
                     /locus_tag="VE646_02430"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HZD78884.1"
                     /translation="MCGDLRASDVGAEVTLCGWVARRRDHGGVTFVDLRDREGLVQLV
                     FHPEEQPQAHAAAQDLKTESVVRATGEVRPRPEGTVNPALPTGEVEVGVRTLEVLAVA
                     ETPPFPVEDRVEASEELRLRYRYLDLRRPEMTRILRLRHTVNRIIREHMERNGFLEVE
                     TPILTRSTPEGARDFLVPSRLWPGTFYALPQSPQQLKQLLMVAGQDRYYQIVRCLRDE
                     TPRADRGLEFTQLDVEMSFVTEEDVFALIEPLYARIVEETQGVKVSVPFPRMTYDEML
                     ARFGSDKPDLRYGMELADLGEVFAGTAFRAFGQVLTQGGVIKGLSAPAGAELSRKELD
                     QLVQVTKERGAAGLVWLVVEGSGIRSPVEKHLGSDEVDGVLKATGASAGDLVLIVADR
                     GDRVDVALDGLRRQLAERLSLIPEGEWAFCWMTEPPLFEWSDDEGKWVSVHHPFTAPA
                     DPEDLDPATARARGYDLVLNGFEVGGGSIRIHRPELQRRVFERLGLSDEEVQEKFGHL
                     LRAFRFGAPPHGGIAMGIDRTVMLMAGKDAIRDVTAFPKAQSGADPMTGAPAPVSEE"
     gene            complement(1726..3000)
                     /gene="hisS"
                     /locus_tag="VE646_02435"
     CDS             complement(1726..3000)
                     /gene="hisS"
                     /locus_tag="VE646_02435"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZD78885.1"
                     /translation="MTDLAPPRGTLDLLPPESGRMRALYERAAELARRFGYRYVETPV
                     FEHTDLFRRTSGGTSDVVAKEMYTFTDRRGRELSLRPEGTAPVMRAYLANQHDQPTPF
                     KAYYLTRMYRYGRPQAGRYREHRQFGIEVFGVAEPGPDVEVIALGDAFLRGLGLSRYE
                     LQINSIGDQVCRPAYRQELIAYFRTHRERLRDEHRDRFEENPLRVLDCKDEACRAVAA
                     EAPKIAEHLCERCREHFEGVLAGLEAEGLKPVVTATLVRGLDYYTRTAFEFVSQVLSE
                     QQGTLFGGGRYDGLAETLGGPPVPGVGFGMGLERVLLALRDEDVAPPEEPGLRVFVVG
                     VGQAGRARASQVVRLLRGAGVASDASFEERPMGAQLRMADRSGAAYAAIVGEREVTAG
                     TVTLRRLTDGEQEVVPQAELVDHLARPGAGGA"
     gene            complement(2997..3614)
                     /locus_tag="VE646_02440"
     CDS             complement(2997..3614)
                     /locus_tag="VE646_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZD78886.1"
                     /translation="MYLDVFSDNSFETNCWMIAADGTDDAVVVDPGFAAQRVRAILEA
                     GGKRPVAVLATHGHYDHIGAAADVCGDDLPLYIHEADALALSDPIAWGAGAGTPPVDV
                     RDVRLLSDGDVLTFAGFRIEVMHTPGHTPGSVCFVTDGSVLSGDLVFAGTIGRSDFAN
                     SSGGDMVRSLERFLRLPDPLPVLPGHGPRTTVGRERAHNPYLVQR"
     gene            complement(3643..>4789)
                     /locus_tag="VE646_02445"
     CDS             complement(3643..>4789)
                     /locus_tag="VE646_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZD78887.1"
                     /translation="SVAEALLADPSARVRSAALERLAGAAPERGVEPALAALHDPDPA
                     VRIAAGRALGSTGILAIDHVLGALDDPRTADAAVQAVRRADGRQAADRIREYVELAAE
                     RATRHGELAAAIPSDGAATDLLREAVLDRGRRTARAALWALTMLEPARGAMEAAIDSL
                     DGERRQLANALETLEAAGDSSFLRPLVALWEAGQPVGAPDRGGPWMEAVLDLEDELIR
                     SCAELVRADGTGGDMARATGALSTVERMLFLRRVPLFADLAPIELEGVAAIAEERGYA
                     DGETIAEEGEVGEELHIVVDGVIRVVREGERELARRSAGDVVGEMSIITRRPRIASLL
                     AQGDVRTIRIERRGFESILRERPNVALGVMRVLAERLAEDTSDPAQA"
BASE COUNT          642 a         1850 c         1662 g          635 t
ORIGIN      
        1 tgctcctcgg acacgggagc cggcgctccg gtcatcggat cggccccgga ctgggccttg
       61 gggaacgcgg tcacgtcgcg gatcgcgtcc ttgccggcca tcagcatgac ggtgcggtcg
      121 atccccatcg cgatgccccc gtggggcggc gcgccgaacc ggaacgcccg aagcaggtgg
      181 ccgaacttct cctggacctc ctcatccgac agtcccagcc gctcgaacac ccgccgctgc
      241 agctcgggcc ggtggatccg gatgctcccg ccccccacct cgaacccgtt cagcacgagg
      301 tcgtacccgc gagcccgcgc cgtggccggg tccaggtcct ccggatcggc gggcgcggtg
      361 aagggatggt gcacgctcac ccacttgccc tcgtcgtcgc tccactcgaa cagcggcggc
      421 tcggtcatcc agcagaaggc ccactccccc tcggggatca gggacaggcg ctcggccagc
      481 tggcgtcgga ggccgtccag ggccacgtcg acccggtcgc cccggtcggc cacgatcagc
      541 accagatcgc cggccgacgc gccggtcgcc ttcagcacgc cgtccacctc gtcggacccc
      601 aggtgcttct cgaccgggga ccggatcccc gagccctcca ccaccagcca cacgagcccg
      661 gcggcgccgc gctccttcgt gacctgcacc agctggtcga gctccttgcg cgacagctcg
      721 gcaccggccg gtgcggacag gcccttgatc accccgccct gggtcaggac ctgaccgaac
      781 gcgcggaacg ccgtccccgc gaacacctcg cccaggtcgg ccagctccat cccgtaccgc
      841 aggtcgggct tgtccgagcc gaagcgggcc agcatctcgt cgtacgtcat gcgggggaag
      901 ggcaccgaga ccttcacccc ctgcgtctcc tccacgatcc gcgcgtagag cggctcgatc
      961 agggcgaaga cgtcctcctc ggtgacgaag gacatctcca cgtcgagctg ggtgaactcc
     1021 aggccccggt cggcgcgggg cgtctcgtcg cgcaggcacc gcacgatctg gtagtagcgg
     1081 tcttgccccg ccaccatgag cagctgcttg agctgctggg gcgactgcgg gagcgcgtag
     1141 aacgtccccg gccacaggcg cgagggcacc aggaagtctc gcgcgccctc gggggtgctg
     1201 cgggtgagga tcggggtctc gacctcgagg aacccgttcc tctccatgtg ctcgcggatg
     1261 atccggttca ccgtgtggcg caggcgaagg atcctcgtca tctcggggcg gcgcaggtcg
     1321 aggtagcggt agcggagccg gagctcctcc gaggcctcga cccggtcctc caccgggaac
     1381 ggtggtgtct ccgcgacggc caggacctcg agggtccgga ccccgacctc cacctcaccg
     1441 gtgggcaggg ccgggttcac cgtgccctcg ggccgcggcc ggacctcccc ggtcgcgcgc
     1501 accaccgact cggtcttcag gtcctgcgcg gcggcgtgcg cctgcggctg ctcctcggga
     1561 tggaagacca gctggaccag gccctctcgg tcccgcagat ccacgaacgt cacgcccccg
     1621 tgatcgcggc gcctggccac ccagccgcac agggtcacct cggcgcccac gtccgaggcc
     1681 cgcaggtcgc cgcacatgtg cgtgcgcagc gaggtggaga ggtcctcagg caccgccggc
     1741 ccctggtcgc gccaggtggt ccaccagttc cgcctgcggc acgacctcct gctcgccgtc
     1801 ggtcagccgt cggagcgtga ccgtcccggc ggtcacctcc cgctcgccca cgatcgccgc
     1861 gtaggcggcg ccggagcggt cggccatgcg cagctgcgcg cccatcggcc gttcctcgaa
     1921 ggaggcatcc gaggcgaccc ccgcgccgcg cagcaggcgg acgacctgcg aggcccgagc
     1981 ccgaccggcc tggcccaccc cgaccacgaa cacgcgtagg cccggctcct cgggcggggc
     2041 cacgtcctcg tcgcgcaggg ccagcaggac ccgctccaga cccattccga accccacccc
     2101 gggcacgggc ggcccgccca gcgtctcggc caggccgtcg taccgcccgc cgccgaacag
     2161 cgtcccctgc tgctcggaga gcacctggct cacgaactcg aacgccgtgc gcgtgtagta
     2221 gtcgagaccc cggaccagcg tggcggtgac gaccggcttc aggccctcgg cctccaggcc
     2281 ggccagcacg ccctcgaagt gctcccggca ccgctcgcag aggtgctcgg cgatcttcgg
     2341 ggcctcggcg gcgacggctc ggcaggcctc gtccttgcag tcgagcaccc ggagcgggtt
     2401 ctcctcgaag cggtcgcggt gctcgtcgcg caggcgctcg cggtgggtcc ggaagtaggc
     2461 gatcagctcc tgccggtacg cgggccgaca gacctggtcg ccgatcgagt tgatctggag
     2521 ctcgtaccgg ctgaggccca gaccccgcag gaaggcatcg cccagcgcga tcacctcgac
     2581 gtcggggccc ggctcggcca cgccgaacac ctcgatcccg aactgccggt gctcccggta
     2641 ccggccggcc tggggccgac cgtaccggta catccgggtg aggtagtagg ccttgaaggg
     2701 cgtgggctgg tcgtgctggt tcgcgaggta ggcgcgcatc accggggccg tgccctccgg
     2761 acggagcgag agctctcgcc cccggcggtc ggtgaacgtg tacatctcct tggccaccac
     2821 gtcgctggtg ccgcccgagg tgcgtcggaa caggtcggtg tgctcgaaca ccggggtctc
     2881 cacgtagcgg tagccgaacc ggcgggccag ctccgcggcc cgctcgtaca gcgcgcgcat
     2941 ccggccgctc tcgggcggga gcaggtccag ggtcccccgg gggggcgcca ggtccgtcac
     3001 cgctgcacca ggtaggggtt gtgggcccgc tcgcgcccca cggtggtcct gggaccgtga
     3061 cctggcagca cgggcagcgg gtcgggcagg cgcaggaacc gctccaggct ccgcaccatg
     3121 tcgccgcccg aggagttggc gaagtcgctc cgaccgatgg tgccggcgaa caccaggtcg
     3181 cccgagagca ccgagccgtc cgtgacgaag cacacgctcc ccggggtgtg gccgggggtg
     3241 tgcatcacct cgatccggaa tccggcgaag gtgagcacgt ccccgtcgga gagcaggcga
     3301 acgtccctca cgtccaccgg gggcgtgccg gccccggcgc cccaggcgat cgggtcggac
     3361 agggcgagcg catcggcctc gtggatgtac agggggagat cgtcgccgca gacgtcggcg
     3421 gcggccccga tgtggtcgta gtgtccgtgc gttgccagca cggccaccgg gcgcttgccc
     3481 cccgcctcga ggatcgcgcg cacccgctgg gcggcgaagc ccgggtcgac cacgaccgcg
     3541 tcgtcggtgc cgtcggcggc gatcatccag cagttcgtct cgaacgagtt gtcggaaaag
     3601 acgtcgaggt acatgggcag gccatcgtag ccgatgcgtg ggctaggcct gggccgggtc
     3661 gctggtgtcc tcggccaggc gctcggccag gacccgcatc acgcccagcg ccacgttggg
     3721 gcgctcgcgc aggatgctct cgaacccgcg gcgctcgatg cggatcgtcc ggacgtcgcc
     3781 ctgggcgagc agggacgcga tccggggacg acgggtgatg atcgacatct cccccaccac
     3841 gtcgccggcg gagcggcggg ccagctcccg ttccccctcg cggaccaccc ggatcacgcc
     3901 gtcgaccacg atgtggagct cctcgccgac ctcgccctcc tcggcgatcg tctccccgtc
     3961 cgcatagccc cgctcctcgg cgatcgccgc cacaccctcc agctcgatcg gtgccaggtc
     4021 cgcgaacagc gggacccgcc gcaggaacag catgcgctcc accgtggaga gcgcgccggt
     4081 ggctcgtgcc atgtcccctc ccgtcccgtc ggcgcggacc agctccgcgc agctgcggat
     4141 cagctcgtcc tccaggtcca ggaccgcttc catccagggc ccgccccggt ccggcgcacc
     4201 gaccggctgg cccgcctccc acagggcgac cagggggcgg agaaacgagc tgtcgccggc
     4261 cgcctccagg gtctccagcg cgttcgccag ctgccgacgc tcgccgtcca gactgtcgat
     4321 cgcggcctcc atggccccac gcgccggctc gagcatcgtc aacgcccaca gggccgcccg
     4381 agcggtccgg cggccccgat ccagcaccgc ctcgcggagc aggtcggtgg ccgctccgtc
     4441 cgacgggatc gcggccgcca gctccccgtg gcgggtggcg cgctcggcgg ccagctccac
     4501 gtactcgcgg atccggtccg ccgcctggcg tccgtcggcc cggcgcaccg cctgcaccgc
     4561 ggcatccgcc gtgcgggggt cgtccagggc cccgaggacg tggtcgatgg cgaggatccc
     4621 cgtgctgccc agcgcgcggc ccgccgcgat ccgcacggcc ggatcggggt cgtgcagcgc
     4681 cgctagggcc ggctcgaccc ctcgctccgg cgcggccccg gccagccgct ccagggccgc
     4741 cgagcgcacc cgggccgagg ggtcggccag gagggcctcc gccaccgag
//
LOCUS       DASQQQ010000055         4757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_86618, whole genome shotgun sequence.
ACCESSION   DASQQQ010000055 DASQQQ010000000
VERSION     DASQQQ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86618"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VE646_02450"
     CDS             <1..660
                     /locus_tag="VE646_02450"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HZD78888.1"
                     /translation="KIVAAALYAVSDAPDRDLRELARALSAPERTEVLRAYVGERGNR
                     RHKPGRAFERTGYRFEVLCDYGAFRDLQRHRPLTLEWQTLTPQHGYLTPEQVDQAGLA
                     DEWHGVMEDSARAWEALADAAGTEVAQYAVSMAYRIRFVMQMSAREAMHLIELRSSPQ
                     GHPTYRRVVHAMHGLIRDVAGHSAVAEAMSHVDYSEVDLERLEAERRAEAKRRASRSA
                     R"
     gene            710..874
                     /locus_tag="VE646_02455"
     CDS             710..874
                     /locus_tag="VE646_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78889.1"
                     /translation="MDRQATLLLALGLLITAAAFVSAAGWILFVRDRRAGRPAPAGAP
                     HAHPEVHEER"
     gene            874..2160
                     /gene="hisD"
                     /locus_tag="VE646_02460"
     CDS             874..2160
                     /gene="hisD"
                     /locus_tag="VE646_02460"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZD78890.1"
                     /translation="MLELLDLRDRRERLEPRRLEIDPDVSDTVRGILARVRDEGDEAL
                     LDLTLELDGADLHERGLIAGPDELRQAERAVPADLKDALDALVERLTDLHRRQLPSEW
                     WEERDGVRFGEIVRPLRRAGCYVPGGRASYPSSVCMTVVPALVAGVEEIVLCTPPNAD
                     GSPDPAVLYAAGRAGAGRVVKVGGAQAIGAMAYGTDSVPAADRIVGPGNTYVTEAKRQ
                     VAGTVGIDGLAGPTELVLIAGQDADPRWVALDLIAQAEHDPAAIATLVSADPVLIERV
                     DRALSAEIGTAGRRDVVQQALAGARAVLVRDLDQAVDVVNDLAPEHLQLSITGPDTFA
                     ARVRNAGAIFVGAFTAVPFGDYGVASNHVLPTAGTARFACGLRSADFVTISSLVELDD
                     RAAARLGPEVAAIARSEGLLGHARAVEIRAEDRSRP"
     gene            2157..3206
                     /gene="hisC"
                     /locus_tag="VE646_02465"
     CDS             2157..3206
                     /gene="hisC"
                     /locus_tag="VE646_02465"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZD78891.1"
                     /translation="MSPVLPRPGLREVAAYESPQPEALARLNTNECPYPLPESFREDL
                     AEAVRHLTLNRYPDREALELRTALAELHDHPLEGTWAANGSNEVIQQLLLAYGGPGRR
                     VVVFEPTYVLHERLTWISHGELVPLPLAEPWTIGSPEVARAFAARPDVVFVCSPNNPT
                     GNAQPPEVIDELAARGEALIVVDEAYVEFGGRSADSLLARYPNLVVVRTFSKAFALAG
                     ARIGYCLASPDVTEDLRRVRLPYHLSALTQTAGLVALAHRDEARAMLDAIRRQRDRIA
                     EALPRLGAEAFRSEANFLLFRPPRSAGEVWRGLLDRGVLVRDMHEVVEGSLRVTAGTP
                     EEVDRFITALEEVLG"
     gene            3203..3790
                     /gene="hisB"
                     /locus_tag="VE646_02470"
     CDS             3203..3790
                     /gene="hisB"
                     /locus_tag="VE646_02470"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HZD78892.1"
                     /translation="MTKREAAVERVTKETRVRVELSIDGSGTARADTGLPFFDHMLQQ
                     LGTHAGFDLAIQAKGDLEVDGHHTVEDVGLALGQALAEAIGDKAGLRRFASITVPLDE
                     AAVAVALDLSGRPFVVHEVPVDAEMIGTYDTELTEDFLRGFATAAAITVHVRLIAGRS
                     PHHVVEAEFKALAKALGDACSLTGRAGTPSTKGAL"
     gene            3787..4398
                     /gene="hisH"
                     /locus_tag="VE646_02475"
     CDS             3787..4398
                     /gene="hisH"
                     /locus_tag="VE646_02475"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HZD78893.1"
                     /translation="MSIGVLDYGMGNLRSVARAIEHVGGTAEIVEDPGAVQRAEALVV
                     PGVGHFGACVRNLRDRGLDQAIRSVAGAGRPVFGVCVGMQVLLEGSEEDPDPGIGLLA
                     GRCRRLPSSASVKVPHMGWNTVAWSAGAHPYLAGVPDGSRFYFVHSFAPDPGPSTLGV
                     AEHGRTFSAVVASANVFATQFHPEKSGSAGLALYEAFVKEAAG"
     gene            4395..>4757
                     /locus_tag="VE646_02480"
     CDS             4395..>4757
                     /locus_tag="VE646_02480"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HZD78894.1"
                     /translation="MIVIPAIDLRGGKCVRLFRGDPNAQTTYEADPVETAVRFEREGA
                     RRLHLVDLDAALGTGSNHEVVVAICRSVAVPVQLGGGLRTLDAVERALEDGAARAILG
                     TAAALDAAFVTKAVERFRD"
BASE COUNT          612 a         1686 c         1793 g          666 t
ORIGIN      
        1 aagatcgtgg ccgccgcgct ctacgcggtc agcgacgccc ccgatcggga cctgcgcgag
       61 ctcgcccgcg ccctgtcggc ccccgagcgc accgaggtgc tccgcgccta cgtgggggag
      121 cgcggcaacc gccgccacaa acccggtcga gccttcgagc gcaccgggta ccggttcgag
      181 gtgctgtgcg actacggggc cttccgagac ctgcagcgcc accgaccgct gaccctcgag
      241 tggcagaccc tcacccccca gcacgggtac ctcacgcccg agcaggtgga ccaggccggc
      301 ctggccgacg agtggcacgg cgtgatggag gactccgccc gggcctggga ggcgctggcc
      361 gacgccgccg ggaccgaggt ggcccagtac gcggtgtcga tggcctaccg gatccggttc
      421 gtgatgcaga tgagcgcccg ggaggccatg cacctgatcg agctccgatc ctcgccacag
      481 gggcacccca cctaccggcg ggtggtccac gcgatgcacg gtctgatccg cgacgtggcc
      541 ggtcactccg cggtcgccga ggcgatgtcc cacgtggact attcggaggt ggacctggag
      601 cggctggagg ccgagcgccg ggccgaggcc aagcgccggg cctcccgctc ggctcggtga
      661 gccctcggcc tggcgacgtg gagggggttt cgggtaccct ggcggcccca tggaccgtca
      721 agcgacgttg ctgctggccc tgggcctgct gatcaccgcc gcggcgttcg tctccgcggc
      781 gggctggatc ttgttcgtcc gcgaccggcg cgcgggtcgt cccgccccgg ccggcgcccc
      841 ccacgcgcat cccgaggtcc acgaggagcg ctagtgctcg agctcctcga cctccgggac
      901 cggagggagc ggctcgagcc ccggcgtctc gagatcgatc cagacgtgtc cgacaccgtg
      961 cgcggcatcc tcgcccgcgt tcgcgacgag ggtgacgagg cgctgctcga cctcaccctg
     1021 gaactggacg gcgccgatct gcacgagcgc ggtctgatcg ccggtcccga cgagctccgg
     1081 caggccgaac gggccgtccc ggccgacctg aaggacgccc tggacgcgct ggtcgagcgg
     1141 ctcaccgacc tgcaccggcg ccagctcccc tccgagtggt gggaggagcg tgacggcgtg
     1201 cgcttcggcg agatcgtccg gcccctgcgc cgggcgggtt gctacgtgcc cggtggccgc
     1261 gcgagctatc cctcgtccgt ctgcatgacc gtggtccccg ccctggtggc gggggtggaa
     1321 gagatcgtgc tctgcacccc cccgaacgcc gacggctcgc ccgacccggc cgtgctctac
     1381 gcggccggga gggccggagc cggccgggtg gtcaaggtgg gcggcgccca ggccatcggg
     1441 gccatggcgt acggaacgga ctccgtcccg gcggcggacc ggatcgtggg ccccggcaac
     1501 acctacgtga ccgaggccaa gcgtcaggtg gccggaaccg tgggcatcga cgggctggcc
     1561 ggacccaccg agctggtcct gatcgccggc caggatgcgg acccccgctg ggtcgcgttg
     1621 gacctgatcg ctcaggccga gcacgacccg gctgccatcg ccacgctggt cagcgccgac
     1681 cccgtgctga tcgagcgggt ggaccgagcg ctctcggcag agatcggaac ggccggacgg
     1741 cgggacgtgg tgcagcaggc gctggccggt gcccgcgccg tcctggtcag ggacctggac
     1801 caggcggtgg acgtggtcaa cgacctggcc cccgagcacc tccagctgtc gatcaccggc
     1861 cccgacacgt tcgcggcacg ggtgcggaac gccggggcca tcttcgtggg agccttcacc
     1921 gcggtgccgt tcggggacta cggggtggcc tcgaaccacg tgctgcccac cgccggcacg
     1981 gcccggttcg cctgcggctt gcgctcggcg gacttcgtca ccatctcctc cctggtcgag
     2041 ctggacgacc gtgcggcggc caggctcgga cccgaggtgg cagcgatcgc ccgcagcgag
     2101 ggtctgctgg gccatgcccg ggcggtcgag atccgggccg aggatcggag ccggccgtga
     2161 gccccgtctt gccccgcccc ggtctgcggg aggtcgcggc gtacgagtcg ccccaacccg
     2221 aggccctggc ccgcctgaac acgaacgagt gcccctaccc gctcccggag agcttccggg
     2281 aggacctcgc cgaggcggtg cggcacctga cgttgaaccg gtaccccgac cgtgaggcgc
     2341 tcgagctccg caccgcgctg gccgagctcc acgaccaccc gctcgaggga acctgggccg
     2401 ccaacggctc gaacgaggtg atccagcaac tcctgcttgc ctacggcggc cccggtcgac
     2461 gggtggtggt gttcgagccc acctacgtgc tccacgagcg gctgacctgg atctcgcacg
     2521 gcgagctcgt ccccttgccc ctggccgagc cctggacgat cggctcgccg gaggtggccc
     2581 gggcgttcgc ggcccgaccg gacgtcgtgt tcgtgtgctc gccgaacaac cccaccggca
     2641 acgcccagcc gccggaggtg atcgacgagc tggccgcccg cggtgaggcg ctgatcgtgg
     2701 tggacgaggc gtacgtggag ttcgggggcc ggagcgccga ctccctgctg gcccgatacc
     2761 cgaacctggt cgtggtgcgg accttctcga aggcgttcgc gctggccggc gcccgcatcg
     2821 gctactgcct ggcgtcgccc gacgtgaccg aggacctgcg gcgggtccgc ctgccctacc
     2881 acctgtcggc actcacccag accgccgggc tggtcgccct ggcccaccgg gacgaggccc
     2941 gggccatgct cgatgcgatc cgccggcagc gggaccggat cgccgaggcc ctgccgcggc
     3001 tgggcgccga ggccttccgc tcggaggcga acttcctcct cttccgtccg cccagatcgg
     3061 ccggcgaggt ctggcgcggc ctgctcgacc gcggtgtgct ggtccgcgac atgcacgagg
     3121 tggtggaggg ctccctgcgg gtcaccgccg gcacgcccga ggaggtcgac cggttcatca
     3181 ccgccctgga ggaggtcctg ggatgacgaa gcgtgaggcc gccgtggagc gggtgacgaa
     3241 ggagacccgg gtccgggtgg agctctccat cgacggctcg ggcacggccc gcgcggacac
     3301 cgggttgccg ttcttcgacc acatgctgca gcagctcggg acccacgcgg gcttcgacct
     3361 cgcgatccag gcgaagggcg acctggaggt cgacggccac cacaccgtcg aggacgtggg
     3421 tctggccctg gggcaggcgc tggcggaggc gatcggggac aaggcggggc tgcggcggtt
     3481 cgcgtcgatc accgtgccgc tggacgaggc ggcggtggcc gtggcgctgg acctgtccgg
     3541 ccggccgttc gtggtgcacg aggtcccggt cgacgccgag atgatcggga cctacgacac
     3601 cgagctgacc gaggacttcc tgcggggttt cgctacggcg gccgcgatca cggttcacgt
     3661 ccggctgatc gccgggcgga gtccccacca cgtggtggag gccgagttca aggcgctggc
     3721 gaaggcgctc ggcgatgcat gctcgctcac cggtcgcgcc ggtacccctt cgaccaaggg
     3781 cgcgctgtga gcatcggggt gctggactac gggatgggga acctgcgcag cgtggcccgg
     3841 gccatcgagc acgtgggggg gaccgcggag atcgtcgagg acccgggcgc ggtgcagcgc
     3901 gccgaggccc tggtcgtgcc cggcgtgggg cacttcggcg cgtgcgtgcg caacctgcgg
     3961 gaccgtgggc tggaccaggc gatccgctcc gtcgccggcg ccggtcggcc ggtgttcggg
     4021 gtgtgcgtgg gcatgcaggt gttgctggag ggcagcgagg aggatcccga cccgggcatc
     4081 gggctcctgg ccggacgatg ccgacggctc ccctcgagcg cgagcgtgaa ggtgccccac
     4141 atggggtgga acaccgttgc ctggagcgcc ggcgcccacc cgtacctggc cggggtgccg
     4201 gacggatccc ggttttattt cgtccactcc ttcgccccgg acccgggtcc tagcaccctc
     4261 ggcgtcgcag agcacggccg aaccttctcg gcggtggtgg cctctgcgaa cgtgttcgcc
     4321 acgcagttcc atccggagaa gtcgggctcg gcggggctgg cgctgtacga ggcgttcgtg
     4381 aaggaggcgg cggggtgatc gtgatcccgg ccatcgatct gcggggcggc aagtgcgtcc
     4441 gcctgttccg cggggatccc aacgctcaga ccacgtacga ggccgacccg gtggagaccg
     4501 cggtgcgctt cgagcgcgag ggtgcccggc ggctgcacct cgtggatctg gacgcggcgt
     4561 tgggcaccgg gtccaaccac gaggtggtgg tggccatctg ccggtcggtg gccgtgccgg
     4621 tccagctggg gggcggcctg cgcacgctgg acgccgtgga gcgggccctg gaggacggag
     4681 cggcccgggc gatcctgggc acggccgcgg cgttggacgc ggcgttcgtg acgaaggccg
     4741 tggagcgctt ccgggac
//
LOCUS       DASQQQ010000056         3277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_142368, whole genome shotgun sequence.
ACCESSION   DASQQQ010000056 DASQQQ010000000
VERSION     DASQQQ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142368"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VE646_02485"
     CDS             <1..1010
                     /locus_tag="VE646_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HZD78895.1"
                     /translation="GARDPDVLVGPQHGVDVGVVRVAEGVATAMTADPVFIVPAYGWE
                     RAAWFAVHILASDAATSGLPLRWMAVDLNLPPEITDQELATMWEAYHRACEDLGIAVV
                     TGHTARYEGCTWPMVGGAVCLASGPEDRFVTPAMARPGDKVVITKGAAIEATALFAAT
                     FPERLAAGVGEDVVRAADALFEQMTVVPEAVVARGFGLREQGVTAMHDATEGGVYGGL
                     TEVAVASGVGLRIDRGAIPVRPEVRAVCEHAGMDPYASISEGTLVCTVVPDRAEDFVA
                     ALAGSGIDAAVVGDVTAPAEGCVLVGEEGVLPLEHPGLDPFWVAFGRWSEEAAGIAKS
                     S"
     gene            complement(1060..1626)
                     /locus_tag="VE646_02490"
     CDS             complement(1060..1626)
                     /locus_tag="VE646_02490"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018090493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZD78896.1"
                     /translation="MAGRIVERGERVVLRRLSAGDGEEFVHRARASRALHRPWASPPT
                     TAAGFEEYLRRARRADTRMFLVCRAGEGDITGVVTISQIFLGNFRSAYLGYYAFEPFA
                     GRGYMREGLRLVMRHAFGTMGLHRLEANVQPGNVASIRLVEGLGFRREGFSERYLKIG
                     GRWRDHLRFAIVAEDFFAQESARRAEPH"
     gene            1670..2542
                     /locus_tag="VE646_02495"
     CDS             1670..2542
                     /locus_tag="VE646_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZD78897.1"
                     /translation="MDLVTVGDLMVDVTVEASALRAGGDVHGTVRLRPGGSAVNAAVW
                     AAASGATARLHGRIGDDLAGRLISEAIGQRGVEAVLAVDPGSVTGSMLVVQEGGERSM
                     VADRGANARLSPADLPAQIEAEAVLVSGYVLLQEGSAAAGRAALDRARARYVAVDAAS
                     WPLVEGVGGERFLRLTEGANVLFANASEARALTGREGEDAADQLAQRYAVVCIKLGGE
                     GAVLSWEGLLIRFGAEEIAEADPTGAGDAFDGAFLAALVSGAAPGDALRLACHAGARA
                     AGSLELWPERESSR"
     gene            2539..>3277
                     /locus_tag="VE646_02500"
     CDS             2539..>3277
                     /locus_tag="VE646_02500"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HZD78898.1"
                     /translation="MSELLRTSEPVRAALAEGRGVVALETSVLAQGLPHPRNLEAAER
                     MSAAIVSRGAIPAWIGVRRGSVVVGLQDTELETFAGRAAPVKVAPRDLPPVLASGRSG
                     ATTVSATVWAAHRAGIPVAATGGIGGVHPGTGDVSADLQELASVPILLVCSGPKSIVD
                     PVATADRLEELGVTAVGYRCERLPFFLAREAPVALEHRVDSAAGAAEVTRIHARLGVR
                     SAILLCNPIPTEQAMDGGEVAAAAGACE"
BASE COUNT          391 a         1083 c         1355 g          448 t
ORIGIN      
        1 tgggggcgcg ggacccggac gtgctggtgg ggccgcagca cggcgtggac gtcggggtgg
       61 tccgggtggc ggagggcgtg gcgacggcga tgacggccga tccggtgttc atcgtcccgg
      121 cctacgggtg ggagcgggcg gcgtggttcg ccgtgcacat cctggcctcg gatgccgcca
      181 ccagcggcct gcccctgcgg tggatggcgg tcgacctgaa cctgccgccc gagatcaccg
      241 accaggagct cgccacgatg tgggaggcgt accatcgggc ctgcgaggac ctcggcatcg
      301 cggtggtgac cgggcacacg gccaggtacg agggatgcac gtggccgatg gtcggggggg
      361 ccgtctgtct ggcctcgggc ccggaggacc gtttcgtcac gccggccatg gccaggcccg
      421 gcgacaaggt ggtcatcacc aagggcgccg cgatcgaggc cacggcgttg ttcgcggcca
      481 cgttccccga gcgactggcg gcgggtgtgg gagaggacgt ggtgcgagcc gccgacgcgc
      541 tgttcgagca gatgaccgtg gtgcccgagg cggtggtggc ccgggggttc ggcttgcggg
      601 agcagggggt gaccgcgatg cacgatgcca ccgagggcgg ggtctacggg gggctgacgg
      661 aggtggcggt cgcttcgggg gtcggtctgc ggatcgatcg gggggcgatc cccgttcgcc
      721 ccgaggtccg ggccgtctgc gagcacgcgg ggatggatcc gtacgcctcg atctcggagg
      781 gcacgctggt ctgcaccgtg gtgcccgatc gggccgagga cttcgtggcc gcgctggccg
      841 gatcggggat cgatgccgcc gtggtgggcg acgtcaccgc gcccgccgag ggctgcgtcc
      901 tggtggggga ggagggtgtg ctccccctgg agcatcccgg gctcgacccg ttctgggtgg
      961 ccttcggtcg gtggtcggag gaagcggccg gcatcgcgaa gagctcctag gccgccctgg
     1021 ccggaagggg gtccgcggag cggcagagcg tgtcgccggt cagtgcggct cggcccggcg
     1081 ggcgctctcc tgggcgaaga agtcctcggc cacgatggcg aagcgcaggt ggtcgcgcca
     1141 ccgtccgccg atcttcaggt atcgctccga gaacccctcg cgccggaagc ccagcccctc
     1201 caccaggcgg atcgaggcca cgttgcccgg ctggacgttc gcctccagac ggtgcaatcc
     1261 catggtgccg aaggcgtggc gcatcaccag ccggagcccc tcccgcatgt agccccgtcc
     1321 ggcgaagggc tcgaaggcgt agtagccgag gtaggcgctg cggaagttgc ccaggaagat
     1381 ctggctgatc gtgaccacac cggtgatatc gccctccccg gcccggcaga ccaggaacat
     1441 cctcgtgtcc gcccgacggg cccgacgcag gtactcctcg aagccggccg cggtggtggg
     1501 cggactcgcc cagggccgat ggagggcccg gctggcccgc gcgcggtgca cgaactcctc
     1561 gccgtccccg gccgacaggc gccgtaggac cacgcgctcc ccccgctcca cgatacgccc
     1621 cgccatcccg ggcaacgcta gcgcgtccac cggatagggt gggccgggca tggacctggt
     1681 gaccgtgggc gacctgatgg tggacgtgac cgtggaggcc tcggccctcc gggcgggcgg
     1741 tgacgtccac ggcaccgtgc ggctgcggcc ggggggaagc gcggtcaacg cggccgtgtg
     1801 ggccgcggcg tcgggcgcca cggccaggct ccacggacgg atcggagacg acctggccgg
     1861 gcggctgatc tcggaggcca tcggccagcg cggggtggag gccgtgctgg cggtcgatcc
     1921 ggggagcgtg accggctcga tgttggtggt ccaggagggc ggcgagcgct cgatggtggc
     1981 cgaccgcggc gcgaacgccc ggctgtcccc ggccgatctg ccggcccaga tcgaggcgga
     2041 ggccgtcctc gtctccggct acgtgctgct ccaggagggt tcggcggccg cggggcgggc
     2101 cgctctggac cgggcccgag cccggtacgt ggcggtggat gcggcgtcgt ggccgttggt
     2161 ggagggggtc ggcggggagc ggttcctgcg gctcacggag ggggcgaacg tgctgttcgc
     2221 caacgcctcg gaggcccggg cgctgacggg acgcgagggc gaggacgcgg cggaccagct
     2281 cgcgcagcgc tacgcggtgg tctgcatcaa gctcggcggg gagggcgcgg tgctgtcctg
     2341 ggaggggctg ctgatccggt tcggggccga ggagatcgcc gaggccgacc ccaccggcgc
     2401 cggggacgcc ttcgacggcg ccttcctggc cgccctggtc tccggcgccg ctccgggcga
     2461 cgccctccgc ctggcctgtc acgccggggc tcgggccgcg ggcagcctcg agctctggcc
     2521 ggagcgagag tcgtcccgat gagcgagctc ctgcgcacgt ccgaaccggt gcgcgccgcg
     2581 ctggcggagg gacggggggt cgtggcgctg gagacgagcg tcctggctca ggggctgccc
     2641 catccccgga acctggaggc cgcggagcgg atgtccgcgg cgatcgtctc gcgcggagcg
     2701 ataccggcct ggatcggggt ccggcggggg tccgtggtcg tcgggctcca ggacacggag
     2761 ctggagacct tcgcggggcg agccgcgccg gtgaaggtgg cgccgcggga cctgccgccg
     2821 gtgctcgcct cgggtagatc gggggccacg accgtgtccg ccacggtgtg ggccgcccat
     2881 cgagccggga tcccggtggc ggccaccggg gggatcggcg gcgtccaccc gggcaccggg
     2941 gacgtctcgg cggacctgca ggagcttgcc tcggtcccga tcctgctggt gtgctcgggg
     3001 ccgaagtcga tcgtggatcc ggtggcgacg gccgatcggc tggaggagct cggcgtgacg
     3061 gccgtcgggt accggtgcga gcgactgccc ttcttcctgg ctcgcgaggc cccggtggcg
     3121 ctcgagcatc gggtggactc ggcggccgga gcggccgagg tgacgcgcat ccacgcccgg
     3181 ctgggggtga gatccgccat cctgctgtgc aacccgatcc cgaccgagca ggccatggac
     3241 gggggggagg tggcggccgc ggcgggggcc tgtgagc
//
LOCUS       DASQQQ010000057         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_114350, whole genome shotgun sequence.
ACCESSION   DASQQQ010000057 DASQQQ010000000
VERSION     DASQQQ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114350"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="VE646_02505"
     CDS             <1..1215
                     /locus_tag="VE646_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005457711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78899.1"
                     /translation="TQYSPVRARECLEEALGIFEGEGMLAERADALAGLVRAVLATDP
                     DDAERVARLALDAATAAADPRLRAGALTQIGQLALHREDALAAIESLTEATALAEEIG
                     DMATLGRAQLMSGHAHALTGELESADRYFDAAMDTAERTGAQMAWLYGAMARVEHWEG
                     RQRFARILEWLEGILPELTARGHRHHLSYVHYAMGYRALRWLGLPAEALEHLDVAAEL
                     TRGDDWNPPAVMFRNGRAALLLDLGRTPDARAAIDEARRIAEEHRLQEATMHYLLVTE
                     ARVALTEGDLERAGNDIARLDGICRDRGEGHAASALNAELALEAGDPSAAIEAARRAV
                     GLEPLIGVNRWFSRLQVLELLARAEVAAGEDPSATLATARGEAEARLAEIPDRYRAGF
                     LSRPEVQAVLSG"
     gene            1305..>2038
                     /locus_tag="VE646_02510"
     CDS             1305..>2038
                     /locus_tag="VE646_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZD78900.1"
                     /translation="MAPQTIALLLAAGFVAGFINTVAGGGSAITLTVLESLLGPTVAN
                     GTNRVAVFLANGVAVAGFARGDAVPWKRALPLIPPTVVGAGIGAWTATRLSNQGMRTA
                     FAFALLFVAVSVLVKPSRWTQQQKEPVLHEPWRSLAFLGVGFYGGFVQAGVGFLILLA
                     VVMGGGLDLVRGNAAKVLLILSYTPVALLLFARAAQVDVKTGLVLAAGNTSGAWVAAR
                     LAVRRGAGWIRWVLVFVAVAAAVWML"
BASE COUNT          270 a          716 c          753 g          299 t
ORIGIN      
        1 acccagtact caccggtgcg agcgagggag tgcctcgagg aggcgctggg gatcttcgaa
       61 ggcgagggaa tgctggcgga gcgggccgat gcgttggcgg gtctggtccg ggcggtcctc
      121 gccaccgacc cggacgatgc cgagcgggtg gcacgcctgg cactcgacgc cgccaccgcc
      181 gccgccgacc cgaggctgcg agccggcgca ctgacccaga tcggtcagct cgcactccac
      241 cgggaggatg ccctggcggc gatcgagagc ctcaccgagg caaccgcgct cgcggaggag
      301 atcggcgaca tggcgaccct gggacgggcc cagctgatgt cgggccatgc ccacgccctg
      361 accggcgagc tcgagagcgc cgaccggtac ttcgacgccg ccatggacac ggccgagcgc
      421 accggagcac agatggcatg gttgtacggg gcgatggccc gggtcgagca ctgggaaggg
      481 cgccagcgct tcgcccggat cctggagtgg ctggagggga tcctccccga gctcactgcc
      541 cggggccatc gtcaccacct ctcgtacgtc cattacgcga tggggtaccg cgcgcttcgc
      601 tggctgggcc tgcccgcgga ggcgctcgag cacctggacg tcgccgccga gctcacccgg
      661 ggcgacgact ggaacccgcc ggcggtcatg ttccgcaacg gacgtgccgc tctcctcctc
      721 gacctgggac ggaccccaga cgctcgggcg gcgatcgacg aggctcgccg gatcgccgag
      781 gagcaccggt tgcaggaggc caccatgcac tacctgctgg tcaccgaagc ccgggtggca
      841 ctgacggaag gggacctcga gcgggcgggg aacgacatcg cccgtctcga cgggatctgc
      901 cgggaccgcg gtgagggtca cgccgcctcg gcgctcaacg ccgagctcgc gctcgaggcc
      961 ggcgaccctt cggccgcgat cgaggcggcc cggcgagcgg tgggcctgga gccgctcatc
     1021 ggcgtcaacc gctggttcag ccgactgcag gtgctcgagc tgctggcgag ggccgaggtc
     1081 gccgccggag aagacccgtc ggcgaccctg gccaccgccc ggggggaggc cgaggcccgc
     1141 ctggccgaga tccccgaccg gtatcgagca ggattcctct cccgtcccga agtgcaggcg
     1201 gtcctgtccg gatagccggg cggctccccg gccccggcgt cccgcggccg gcgggcattg
     1261 cgccggaccg ctcctcggcg caggtatggt gccgcaccac cccgatggct ccgcaaacga
     1321 tcgcactgct gctcgccgcc ggtttcgtcg ccggcttcat caacaccgtc gccggcggcg
     1381 gctccgccat cacgctcacg gtcctcgagt cgttgctcgg gcccacggtc gccaacggca
     1441 ccaaccgggt ggcggtgttc ctcgccaacg gtgtggcggt ggcagggttc gcccggggag
     1501 acgcggtccc ctggaagcgg gcgctcccgc tgatcccgcc caccgtcgtc ggagccggga
     1561 tcggggcgtg gaccgcgacg cggctctcca accaggggat gcggacggca ttcgccttcg
     1621 cactgttgtt cgtggcggtc tcggtgctgg tcaagccttc ccgatggacc cagcagcaga
     1681 aggaaccagt gctccacgag ccgtggcgct cgctggcctt cctcggggtc ggcttctacg
     1741 gaggcttcgt tcaggcaggg gtggggttcc tcatcctcct cgccgttgtc atgggtggag
     1801 gtctggacct ggtccggggg aacgccgcca aggtgttgct gatcctctcg tatacgccgg
     1861 tggcgctcct gctcttcgcc cgggcggccc aggtggacgt caagaccgga ctggtgctgg
     1921 cggccgggaa cacctccggc gcctgggtgg ccgcccgcct ggcggtgcgg aggggtgccg
     1981 gctggatccg gtgggtgctg gtgttcgtcg ccgtcgccgc cgcggtgtgg atgctgat
//
LOCUS       DASQQQ010000058        17370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_86978, whole genome shotgun sequence.
ACCESSION   DASQQQ010000058 DASQQQ010000000
VERSION     DASQQQ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 17370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17370
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86978"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VE646_02515"
     CDS             complement(<1..569)
                     /locus_tag="VE646_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZD78901.1"
                     /translation="MTAADRERAEAKLRVEELREQLNEHAYRYYVLDDPVVSDAEYDE
                     LMNELKALEERFPELVTPDSPTQRVGVAPTELFAPVQHRAVMLSLDNVFSLEELQAWA
                     ARAERGVGSGARYACELKIDGVAVALTYERGVLVRGATRGDGRVGEDITANVRTVRSV
                     PRRLQVAEPPEVLEIRGEIYLPVRAFENL"
     gene            complement(569..1243)
                     /locus_tag="VE646_02520"
     CDS             complement(569..1243)
                     /locus_tag="VE646_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78902.1"
                     /translation="MRLLRKKPRSFDIQVQDMLLHISAGGDVNEESRAAALSFWEQLQ
                     SYALRAPEVRTSKRPIEVRDDAPAVVRELVGAARAAGVGPMFSFRGALTDHVGRFLAP
                     STSDLTVSCGGDYFIVARKRRKLTVHRAPDGRTIAVVVPPSRQGVGISTAFTSGRAPV
                     DGLAVLATSCMLADAAAAGVQALLSKPDGFSLALGYLKRVPGVTGGLLVQGHRIGLAG
                     GVEIAA"
     gene            complement(1320..2399)
                     /gene="mnmA"
                     /locus_tag="VE646_02525"
     CDS             complement(1320..2399)
                     /gene="mnmA"
                     /locus_tag="VE646_02525"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HZD78903.1"
                     /translation="MASRGSVLVAMSGGVDSSVAACLLAEQGYEVLGSHLRLVHLADG
                     VDHGCCGPTARADAAEVARIAGFPFEIVDLSRAFEDTVLADFLAEHRAGRTPNPCARC
                     NEQIKFGAFLARADELGMDFVATGHYVRTRRDAAGVWHLLRGVDRGKDQSYMLHALGQ
                     RELARSLFPVGGLPKAETRAHARRLGLPVASKPDSQELCFVPRGDHGDFLEGLAPELL
                     RQGQIVDAAGRVVGRHRGLPRYTIGQRRGLGISTGARTYVLDVDAAANRVVVGPGELL
                     ERRGLVADRVRWVIGRPPARGPFQAEVRYRYRGADVPAVVEAEGDRVRVRFGSPQRGV
                     APGQSVVLYRGEELLGGARIVGSIR"
     gene            2488..3252
                     /locus_tag="VE646_02530"
     CDS             2488..3252
                     /locus_tag="VE646_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZD78904.1"
                     /translation="MNVVVLVKYVPDPQGTPELGEDFLVKREGTEGALDPGDEYAVEA
                     ALQVAGGDGEVALVSMGPEVALTAIRRGLSMGAHRGVLVSDPSLRGADVLATARVLAA
                     VVRRAPVDLVLAGVESTDGYTGTLPMTVAELLGLPAATFARKLDVVDGTLRIERQTEG
                     GFDVVEAPLPALATVTASAAEPRYPTLKGIMQAKQKPLEQLSLTDLGVEPAEVTGTQR
                     VTDVRPAAEKGSGEIVQAGEDGAGRIVQFLAEAKVI"
     gene            3253..4212
                     /locus_tag="VE646_02535"
     CDS             3253..4212
                     /locus_tag="VE646_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HZD78905.1"
                     /translation="MAGVWVYAQVEPEGPEASALELLTKARELSDQVAAVALGPGATA
                     AAASLGEHGAATVYASDDEVYAECLGQPAAHALDALIREHRPDLVLFPSSYEARDVAG
                     RLQARTGSTLMANATDVPSVHGAQTQIFGGTAVVDVALEGPAPHLVLVRPKSFPAEPS
                     GGTAQVVPVDLQIPDELRRARRIERHVEAAAGPRLEQAKVVIAGGRGLQDPSNFAILE
                     ELASLLGDSAVGATRAVVDAGWVPYSYQIGQTGKTVKPEVYLAVGVSGATQHVVGMKG
                     SKRIVAINKDPDAPIFKLADLGVVGDALAVLPELIQELRARSG"
     gene            4216..5994
                     /gene="pabB"
                     /locus_tag="VE646_02540"
     CDS             4216..5994
                     /gene="pabB"
                     /locus_tag="VE646_02540"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HZD78906.1"
                     /translation="MVEARFDDLRPGRERSFRLVEPLGSVQAREPSAVPDALDRVEAA
                     VGRGLWAGGFVAYEAAPGLDPALTVRSTRHPSDPLRGLPLVWFGLFRRCERVPPLRPE
                     AHGPVDRDLGWRPSVGREQYEDAVSLIRERIAAGDTYQVNHTFRLRTRVPGSPVELYR
                     DLCLAQRGGYAAFLDAGGYLVLSASPELFLRIEGDRITTRPMKGTTPRGRWPAEDARA
                     ARALQRSSKDRAENAMIVDLLRSDLGRIARPGTVRADPMFEAERYETLWQLTSTITGA
                     TRPGTRLRDVFAALFPSGSVTGAPKVAAMRLICELEDSPRGIYCGSIGYLAPEGSGEP
                     RANLNVAIRTVVLDRRSGAAEYGVGGGITYDSSSSGEYREALAKTRVLTARRPEFQLL
                     ETILHRGRGGFRHLHEHLARLRGSAGYFGFALDPEAVELRLKQARAELGDADGLVRLL
                     LARDGTVQVRIRPRPAPHPGPVTVALDTDPVDPSDVWLFHKTTLRERYERRRAARPDV
                     DDVLLINTRGEVTESTIANLAVRLDGRWVTPPLASGLLPGAYRDRLVRGGRLRERPIS
                     LEEVSAAPGLALVNSARGWRSARLRG"
     gene            complement(6069..6275)
                     /locus_tag="VE646_02545"
     CDS             complement(6069..6275)
                     /locus_tag="VE646_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78907.1"
                     /translation="MPSDASYRGARVRELPKANRTYGSGRVCAAEGCGTKLSMYNRWD
                     FCWQHEPVHEYVPRGKRRSRREAA"
     gene            complement(6414..6902)
                     /locus_tag="VE646_02550"
     CDS             complement(6414..6902)
                     /locus_tag="VE646_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZD78908.1"
                     /translation="MKVRRATAEDVPVLVGLFQELERLQGDWRVFTPRPGFYDEIGAK
                     YQRALDTENAVVLVAEDDGEVVGMAYGEVRIPSRFSDERALELSGVVVRAGYRGRGVG
                     RALVKEASSFAQEIAVDWVELKTFAPNQGAMEFWESLGFTPRVVQLTQATKALARNLE
                     RD"
     gene            complement(6913..7686)
                     /locus_tag="VE646_02555"
     CDS             complement(6913..7686)
                     /locus_tag="VE646_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZD78909.1"
                     /translation="MGEFVRIEADGAVATIRLDRPPANVLNERMSRELWDSSREVAGD
                     DAIRAVVVWGGQRRFSGGADITEMVGFGPREVDPVVGALEGALRHMEAIPKVVIAAVN
                     GYALGGGCEVALAADFRFAAEDAQFGQTEIALGIIPGAGGTQRLPRLIGLARAREMIY
                     TGRRVSADEALAIGLVDRVFAPDEVYPAALEEAARYAEGPSLALRAAKMAVNAAAQTD
                     QERGLELERGVFRDLFVSLDQEEGMRAFLEKRRPRFEGR"
     gene            8351..8722
                     /locus_tag="VE646_02560"
     CDS             8351..8722
                     /locus_tag="VE646_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZD78910.1"
                     /translation="MSSAAGSAPLGGEALLHFDVRGDNLCFRDGRALLVDWNWACVGN
                     PMVDVAFWLPSLRAEGGPRPEDVPASGAAELAAACAGFFRAHAARPPIPTAPHVRPLQ
                     LRHARTALPWAARALGLPSPA"
     gene            8763..9191
                     /locus_tag="VE646_02565"
     CDS             8763..9191
                     /locus_tag="VE646_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HZD78911.1"
                     /translation="MSQRSADVVAILKDHGLRMTPQRRAIVAAVMRTTGHISPSTLAR
                     RVQADMPGVNASTIYRTLALLEEVGVLAHAHLEGGAEYHRAEQPPHAHLACSRCGAEI
                     ELSLDATRELERLIVERHDFAPDLTHFAISGLCRDCRSRG"
     gene            complement(9217..10122)
                     /locus_tag="VE646_02570"
     CDS             complement(9217..10122)
                     /locus_tag="VE646_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD78912.1"
                     /translation="MTASLDAEVRAQTRDRLDAPKGEQLMVCRDLRFAYLERFPALEG
                     VSLSIRRGEKVALLGANGSGKSTLLKILDGLLFPDSGTYEAFGQLVTEDNLEDEQFSM
                     GFRSRVGFIFQNSDAQVFSPTVREEIAFGPLQMGMAPKRVERRIADVMEMLGIQGLAD
                     RAPFQLSGGEKKRVAIASVLVMNPEVLLFDEPTAALDPRTQQWLLELIVELNRAGKTI
                     VLATHDLDVLDIMADRCLVFSEDHRLVGEGAPGRVLADHELLLRVNLVHEHSHAHGLD
                     IHLHQHDYRHHEGSGGSETGAAPKG"
     gene            complement(10100..10993)
                     /gene="cbiQ"
                     /locus_tag="VE646_02575"
     CDS             complement(10100..10993)
                     /gene="cbiQ"
                     /locus_tag="VE646_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HZD78913.1"
                     /translation="MQAEVGLCPCGCIGRRTKGSFVEKTLNGASNIMRQAMFGDDIAR
                     RHGLLQRVDARIKIAATVLLLLAAALVHHIPALLVMYGITLVLAAVSALPVGFFVKRV
                     WLFIPIFTGIVVLPATFSFITPGTIVLPLWHWSGHQVGITAQGLGSAGLIVMRVATSV
                     SLVVLLTLTTPWVKLLAGLRALFVPRIFILIIGMAYRYVFLLLNSVTDMYTARKARSV
                     GKGENAKEGQRFVAATAGALFGKAHALSEEVHMAMLSRGYQGDAKTLEGFRVRAVDLA
                     VALISLMLAVLLVGGDRLVGR"
     gene            complement(11044..12132)
                     /gene="cbiM"
                     /locus_tag="VE646_02580"
     CDS             complement(11044..12132)
                     /gene="cbiM"
                     /locus_tag="VE646_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HZD78914.1"
                     /translation="MHIPDGYLSPATCATLGAATVPFWYQAARRVRKVVKSRYVPLVA
                     IGAAFSFLVMMFNIPVPDGTTAHAVGAVMIAVVLGPWAAVIAVSIALAIQALFFGDGG
                     VLAFGANAFNMAVLMPFAGYAVYRLLSRHLSLTSPKRALAAGVGGYAGINVAALAAAV
                     EFGLQPTLFHTANGTPLYAPFHLAQTIPAMMLAHLTVAGIAEFVVAAGVIGYLQRANV
                     PVLRLNHPKVPVTDEELERRKRLGWRWVLVGMGVMIVLTPLGLLAPGGAFGEAAPANL
                     DLGRYNLRAVPAGLERFSSFWSHTLLGGYGFESGNHAALAYILSAVLGILVIGTVMLG
                     FVVVWRLLRRRRGGGPERPTRDVEEAAA"
     gene            complement(12245..13897)
                     /locus_tag="VE646_02585"
     CDS             complement(12245..13897)
                     /locus_tag="VE646_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZD78915.1"
                     /translation="MAERDRWEDDYRSSPERDADFQTLSGETLDPLYDPSDVHGLDPD
                     RDLGYPGRFPYTRGVYPTMYRGRLWTMRQFAGFGSPAETNARYKYLLEAGQTGLSVAF
                     DMPTLMGRDSDDPRSEGEVGRCGVATDSLADFETLFAGIPLGDITTSMTISGPAVILF
                     AFFLATAERQGVPWSALYGTLQTDILKEYIAQKEWIFPPRPHLRLIGDLMEFCADQVP
                     KFHPISVSGYHIREAGANAWQELAYTLADGFAYVELGRQRGLEVDRFAPGLSFFFNAH
                     IDFFEEIAKYRAARRIWARWLQGRYGARDDASLRLRFHTQTAGVSLTAQQPMNNVVRT
                     AIEALAAVLGGTQSLHTNALDEVLALPTEDAAKLALRTQQIIAHETGVPRVIDPLGGS
                     YFVESLTGRMEELAEEEFARIERMGSGGMLEGVLAGIESGYFQQEIAESAWREQERYE
                     KGRLVKVGVTDFVEPSETPIPTLVIQPDMEQRQVDRLREVRASRRSQPAEASLARLRE
                     AAGTERNLMPLLVDCARAMCTEGEIVAALRDVFGEHLETPRF"
     gene            complement(13925..14860)
                     /gene="meaB"
                     /locus_tag="VE646_02590"
     CDS             complement(13925..14860)
                     /gene="meaB"
                     /locus_tag="VE646_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HZD78916.1"
                     /translation="MELADGVLAGDPRAVARAISMVEDGAEGLEELSAALFGRTGHAY
                     TLGLTGAPGVGKSTLAAELVTAGRERGLRVAVLAIDPTSPFTGGALLGDRLRMQAHAT
                     DPGVFIRSMATRGHLGGMALAAPEAVRILDASGTELVIVETVGVGQAEVDVAAATDTT
                     LVVVSPGWGDAIQVAKAGVLEIADVFVVNKADREGAEAAARDLRQMIRMGPERPWSVP
                     VVQTSAVTPDGIEELWEAIAAHRAHLEDGDRLGALRRARLLREVQSLAAERLRSSIAD
                     LIGSDPGFAEDLEERRTDPYRAAAILVERASGGGG"
     gene            complement(14903..15316)
                     /locus_tag="VE646_02595"
     CDS             complement(14903..15316)
                     /locus_tag="VE646_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78917.1"
                     /translation="MRVEYAEPEPQGLAAMVGGLIEANLARHPERVRLLRPAVIDLSA
                     PDAAVSVTLSIEPGRIRVSSGDHPSVRSHVHVRAPAEDLLLLASVPLRFGLPDPLRRG
                     GRTVLARVIRGRIRIQGLVRHPSTLSRLSRLLSVV"
     gene            complement(15313..16353)
                     /locus_tag="VE646_02600"
     CDS             complement(15313..16353)
                     /locus_tag="VE646_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZD78918.1"
                     /translation="MRAVVYRGIGRVTVEEVPEPEVEDSRDAVVRVGVSAICASDLHF
                     LHGKAPLDPGDVMGHEAMGVVQSVGSAVERFRPGDRVAVAFHIACGDCWFCRRGQTQL
                     CDEWRMLGGGLFGGELGGTQAELVRVPSADVNLLALPDRIGDEPAVFLGDVLTTGVYG
                     ASVAGIEPGDAVAVVGVGPVGHFCVQAARSHGPSELLALDLEPERLALATRFGATAID
                     VRAHHPHTAVAERTGGRGADVVIEAVGTPQAFASALDVVRRGGRIAVVGMFTSETMPM
                     QAGVWWTRALEVRFAGICPVHAHWDRARDQVLAGDLDPMPVVSHRLQLDEAPLGYELF
                     ASRRATKVLLRP"
     gene            complement(16378..16806)
                     /locus_tag="VE646_02605"
     CDS             complement(16378..16806)
                     /locus_tag="VE646_02605"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HZD78919.1"
                     /translation="MFPSDAWFAIYVDRINGSSDYRESASDWEGDIAFVFEAEPDRNV
                     PQDIWGWLDLWHGTCRAGRIVTPEEGERAPYVIRAPYSRWKEVLAGDLDPVKGMMQGK
                     LKLQGDLPTIIRYVRAANELVHLTMSVPTEFLDERPPQTA"
     gene            complement(16848..>17370)
                     /locus_tag="VE646_02610"
     CDS             complement(16848..>17370)
                     /locus_tag="VE646_02610"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZD78920.1"
                     /translation="SRFHREWRQAIGFPRRLWPEVRSGVGFGIVLYPVITLGVGLLVT
                     VVLSAVSGHQPEAPQQVPPHLSALGVAVAIVYAVIIAPFHEEFFFRGILFRSLRDRYG
                     FGVGAVGSGLAFGLIHYIPSTWYDSALLMTVMVFTGIALAWLYDRRGNVGANMVAHAT
                     FNVIGLVLILALR"
BASE COUNT         2604 a         6499 c         5886 g         2381 t
ORIGIN      
        1 ttgaggttct cgaacgcccg cacgggcagg tagatctcgc ccctgatctc cagcacctcg
       61 ggaggctccg cgacctgcag ccggcgaggc accgaccgga ccgtccggac gttcgcggtg
      121 atgtcctccc ccacccgacc atcgccccgg gtggcgcccc ggaccaggac cccacgctcg
      181 taggtgagcg ccaccgccac cccgtcgatc ttcagctcgc acgcgtaccg ggcccccgag
      241 ccgacccccc gctccgcgcg cgcggcccag gcctgcagct cctcgagcga gaagacgttg
      301 tccagtgaca gcatcaccgc tcgatgctgc accggcgcga acagctcggt cggtgctacc
      361 ccgacccgct gggtggggct gtcgggcgtg accagctccg ggaagcgctc ctcgagcgcc
      421 ttgagctcgt tcatcagctc gtcgtactcg gcgtcggaga cgaccgggtc gtccaggacg
      481 tagtagcgat aggcgtgctc gttcagctgc tcccgcaact cctccacgcg gagcttggcc
      541 tcggcccgct cgcggtccgc cgctgtcatc aggcggcgat ctccacgccg cccgccaggc
      601 cgatccggtg cccctgaacc agcaggcccc cggtcacccc cggcacgcgc ttcaggtagc
      661 ccagggccaa gctgaacccg tcgggcttgg acagcagggc ctggacgccg gccgcagcgg
      721 cgtcggccag catgcaggag gtggccagca cggccagacc gtcgacgggc gcgcggcccg
      781 aggtgaacgc ggtggagatc ccgacgccct gccgactggg cgggaccacc acggcgatgg
      841 tgcggccgtc gggggcgcga tggaccgtca gcttgcggcg cttgcgggcc acgatgaagt
      901 agtcgccgcc gcacgagacg gtcagatccg aggtggacgg ggccaggaac cgccctacgt
      961 ggtcggtgag ggctccccgg aacgagaaca tcggccccac ccccgcggcc cgggccgccc
     1021 ccaccagctc gcgtaccacc gccggagcgt cgtcccggac ctcgatgggc cgcttgctgg
     1081 tgcggacctc gggtgcgcgc agcgcgtacg actgcagctg ctcccagaac gacagcgccg
     1141 ccgctcggga ctcctcgttc acgtccccgc cggcgctgat gtgcaggagc atgtcctgca
     1201 cctggatgtc gaagctgcgt ggcttcttcc tgagcagcct cacgccagca cctcgagcac
     1261 ctctcgagcc cgtctccccg tctcgggcgg gatgatagcc gagcccggcg acgccctcgt
     1321 cagcggatcg acccgacgat ccgagcgccg cccaggagct cctccccccg gtacaggacc
     1381 acgctctggc ctggggccac gccccgctgc ggggatccga accgaaccct gacccggtcg
     1441 ccctccgcct ccaccacggc cgggacgtcc gcccctcggt accggtaccg gacctccgcc
     1501 tggaaggggc cccgagccgg gggcctgccg atcacccacc ggacccggtc ggccaccagg
     1561 ccccggcgct cgaggagctc gccgggtccc accaccaccc ggttggcggc cgcatcgacg
     1621 tcgagcacgt aggtccgggc tcccgtggag atccccaatc cccgtcgctg cccgatcgtg
     1681 tagcgaggca ggcctcgatg acgccccacc acccggccgg cggcgtcgac gatctggccc
     1741 tgtcgaagca gctcgggcgc caacccctcc aggaagtcgc cgtggtcgcc ccgcggtacg
     1801 aagcagagct cctgcgagtc cggcttggag gccaccggca accccagccg ccgggcatgc
     1861 gcgcgcgtct ccgccttcgg cagtccaccc accgggaaca gcgaacgggc gagctcccgc
     1921 tggccgagcg cgtgcagcat gtacgactgg tccttgcccc ggtcgacccc ccgcagcagg
     1981 tgccagacgc cggcagcgtc ccggcgggtc cgcacgtagt gccccgtggc cacgaagtcc
     2041 atgcccagct cgtcggcccg ggccaggaac gccccgaact tgatctgctc gttgcagcgg
     2101 gcgcaggggt tcggggtccg gccggcccgg tgctcggcga ggaagtcggc cagcaccgtg
     2161 tcctcgaagg cacggctcag gtccacgatc tcgaagggga agccggcgat cctggccacc
     2221 tccgcagcgt ctgccctggc ggtcggtccg cagcatccgt ggtcgacccc atcggccagg
     2281 tgcaccagcc ggaggtgcga gccgaggacc tcgtacccct gctcggcgag caggcaggcc
     2341 gcgaccgacg agtccacccc gccggacatc gccaccagca ccgatcccct cgacgccatc
     2401 gggttggatg ctacgacgcc gagctgtggg gcacgccccc cggggcgtgg gtatgctcgt
     2461 cgtgtcgtcc gaccggagga ccgaggcatg aacgtcgtcg tcctggtgaa gtacgtgccc
     2521 gacccccagg gaacccccga gctgggggag gacttcctgg tgaagcggga gggcaccgag
     2581 ggggcgctcg accccggcga cgagtacgcg gtggaggccg cgctccaggt ggccggcggc
     2641 gacggcgagg tggccctggt ctcgatgggg cccgaggtgg ccctgaccgc gatccggcgg
     2701 ggactctcca tgggggctca ccggggggtg ctggtgagcg acccgtcgtt gcggggagcc
     2761 gacgtgttgg ccacggccag ggtcctggcc gcggtggtgc gtcgggcgcc cgtcgacctg
     2821 gtcctggccg gcgtcgagtc caccgacggc tacacgggca cgctgccgat gaccgtagcg
     2881 gagctcctcg gcctgccggc cgccacgttc gcccgcaagc tcgacgtcgt cgacgggacc
     2941 ctccggatcg agcgacagac cgagggcggc ttcgacgtgg tggaggcccc cctgccggcc
     3001 ctggccaccg ttacggccag cgccgcggag cctcggtacc ccacgctgaa gggcatcatg
     3061 caggccaagc agaagcccct cgagcagctg tcgctgaccg atctcggggt ggagccagcc
     3121 gaggtgaccg gcacgcagcg ggtgaccgac gtgcgtcccg cggcggagaa ggggtccggc
     3181 gagatcgtcc aggcgggcga ggacggcgcg ggccggatcg tccagttcct ggccgaggcc
     3241 aaggtgatct gaatggccgg ggtgtgggtc tacgcgcagg tcgagccgga gggcccggag
     3301 gcctccgcgc tggagctgct gacgaaggca cgcgagctct cggaccaggt ggccgcggtc
     3361 gccctcggcc cgggagccac cgcggccgct gcgagcctgg gggagcacgg ggcagccacg
     3421 gtctacgcct ccgacgacga ggtgtacgcg gagtgcctgg gacagcccgc ggctcacgct
     3481 ctggacgcgc tgatccggga gcaccggccc gacctggtgc tcttcccctc gagctacgag
     3541 gcccgggacg tggccgggcg gctccaggcc cggaccggct ccacgctcat ggccaacgcc
     3601 accgacgtgc cgagcgtgca cggcgcgcag acgcagatct tcggcggcac ggcggtcgtc
     3661 gacgtggcgc tggagggacc cgcaccccac ctggtgctgg tgcggcccaa gtcgttcccg
     3721 gccgagccct cgggtggtac ggcgcaggtg gtgccggtcg acctccagat ccccgacgag
     3781 ctccgtcgcg cccgccggat cgagcggcac gtggaggcgg cggccgggcc ccggctggag
     3841 caggcgaagg tcgtgatcgc cggcggccga gggctgcagg atccatcgaa cttcgccatc
     3901 ttggaggagc tggcctcgct gctcggcgac tccgcggtgg gggccacccg ggcggtggtc
     3961 gacgccggat gggtgcccta cagctaccag atcgggcaga ccggcaagac cgtgaagccc
     4021 gaggtgtatc tggcggtggg ggtgagcggc gccacccagc acgtggtggg catgaagggc
     4081 tcgaagcgga tcgtggcgat caacaaggac cccgacgccc cgatcttcaa gctcgcggac
     4141 ctcggcgtgg tgggcgacgc gctcgccgtg ctgcccgagc tcatccagga gctgcgggcc
     4201 cgctcgggct gatcggtggt cgaggccagg ttcgacgatc tccgtccggg acgggagcga
     4261 tccttccggc tggtggagcc gctgggatcg gtccaggcgc gggagccctc cgcggtcccg
     4321 gatgccctgg accgggtgga ggccgccgtg gggcgaggcc tatgggccgg cgggttcgtc
     4381 gcctacgagg ccgcgccggg actcgatccc gccctgaccg tccggtcgac acggcacccc
     4441 tccgacccgc tgcgaggact gccgctcgtg tggttcgggc tcttccggcg gtgcgagcgg
     4501 gtgccccccc tgcgaccgga ggcccacgga ccggtcgacc gggacctggg atggcggccg
     4561 tccgtcggca gggagcagta cgaggacgcc gtctcgctga tccgcgagcg catcgccgcg
     4621 ggcgacacct accaggtgaa ccacacgttc cggctacgga cccgggtgcc gggatcgccc
     4681 gtcgagctgt accgcgacct ctgcctggcc cagcggggtg ggtacgccgc cttcctggat
     4741 gccggaggct acctggtgct ctcggcctcg cccgagctct tcctgaggat cgagggcgac
     4801 cgcatcacca cacgccccat gaaggggacg accccccgcg gacggtggcc ggcggaggat
     4861 gcccgggccg ctcgggccct ccagcgctcg tccaaggacc gtgccgagaa cgccatgatc
     4921 gtcgatctgc tccgaagcga cctgggccgc atcgcccggc cggggacggt gcgcgcggat
     4981 ccgatgttcg aggccgagcg gtacgagacg ctctggcagc tcacctccac gatcaccggc
     5041 gcgacccgcc ccggcacgcg gctgcgcgac gtgttcgcgg ccctgttccc cagcggttcg
     5101 gtgaccggtg cccccaaggt cgccgcgatg cgcctgatct gcgagttgga ggattccccc
     5161 agggggatct actgcgggtc gatcgggtac ctggcgcccg agggctcggg agagccccgc
     5221 gccaacctca acgtggccat ccggacggtg gtgctggacc ggcggagcgg tgcggccgag
     5281 tacggcgtgg gcggggggat cacctacgat tcgtcgtcct ccggcgagta ccgtgaggcg
     5341 ctcgccaaga cccgtgtcct caccgcccga cgtcccgagt tccagctgct ggagacgatc
     5401 ttgcaccggg gccggggcgg gttccgacac ctccacgagc acctggcccg cctgcgcggg
     5461 tcggccgggt acttcggctt cgcgctggac ccggaggccg tggagctgcg gttgaagcag
     5521 gcgagggcgg agctgggaga cgccgacggg ctggtccgcc tgctgctcgc ccgcgacgga
     5581 acggtccagg tccggatccg accacggccg gccccccatc cggggccggt caccgtcgcc
     5641 ctcgataccg atccggtgga tccctccgac gtctggctgt tccacaagac cacgctgcgt
     5701 gagcgctacg agcgacggcg tgccgcacgc cccgacgtgg acgacgtgct cctgatcaac
     5761 acccggggcg aggtgaccga gtccacgatc gcgaacctgg ccgtgcgcct ggacgggcga
     5821 tgggtcacac cgcccctggc atctgggctg ctgccgggcg cctaccggga ccggctggtg
     5881 cgcggcggcc ggctgcgcga acgaccgatc tcgctggagg aggtcagcgc cgcacccggg
     5941 ctggcgctgg tgaactcggc ccgcggatgg cgatcggcgc gcctgcgggg atgagcgcgg
     6001 acccagccgt cgctcgtcca ggagaacagg cgtgaggcct cggtgggccg gtcagcctgc
     6061 ggaccggctc aggcggcttc cctgcgcgac cggcgcttgc cccgcggcac gtactcgtgg
     6121 acgggctcgt gctgccagca gaagtcccag cggttgtaca tcgagagctt cgtgccgcag
     6181 ccctcggccg cgcacacgcg gcccgagccg tacgtccggt tcgcctttgg gagttcgcgg
     6241 acgcgagctc cgcggtagga agcatccgaa ggcatgacca ctcctcgcgt tcgtccggac
     6301 cctgtccatc gctccagggt catcaggtat tcgaacagcg cggaaggtcg gttcgttccc
     6361 ggatggggac ggttcgcgca gatccacagc ggatgcacag gttcggggcg cgctcagtcc
     6421 cgctccaggt tcctggccag cgccttcgtg gcctgggtga gctggaccac cctgggcgtg
     6481 aaccccaggg actcccagaa ctccatggcc ccctggttgg gggcgaaggt cttgagctcc
     6541 acccaatcca ccgcgatctc ctgcgcgaag ctcgacgcct ccttcaccag ggcgcgtccc
     6601 accccccgtc cccggtagcc ggcccggacc accacgccgg agagctccag ggcccgttcg
     6661 tcggagaacc gggaggggat ccgcacctct ccgtaggcca tccccacgac ctcgccgtcg
     6721 tcctcggcca ccagcaccac cgcgttctcc gtgtcgaggg cccgctggta cttggcgccg
     6781 atctcgtcgt agaagccggg gcgcggcgtg aacacccgcc agtcgccctg cagccgctcc
     6841 aactcctgga acaggccgac cagcaccggg acgtcctcgg cagttgcccg tctcaccttc
     6901 acggcttccc ccctaccggc cctcgaacct cggacggcgc ttctctagga acgcccgcat
     6961 gccctcctcc tgatcgagcg agacgaagag gtcccggaac accccacgct caagctcgag
     7021 cccccgttcc tgatccgtct gcgccgccgc gttcaccgcc atcttcgccg ctcgcaaggc
     7081 gagagacgga ccctccgcgt agcgggcggc ctcctccagc gccgccgggt agacctcgtc
     7141 gggcgcgaac acccgatcca ccagcccgat ggccagcgcc tcgtccgcgg acacgcggcg
     7201 tcccgtgtag atcatctctc gggccctggc cagcccgatc agccgaggca gccgctgcgt
     7261 gccgccggcg cccgggatga tccccagcgc gatctcggtc tggccgaact gggcgtcctc
     7321 ggccgcgaac cggaagtccg ccgccaaagc cacctcgcag ccacccccca gcgcgtaccc
     7381 gttcacggcc gcgatcacca ccttcgggat cgcctccatg tgccggagcg ccccctccag
     7441 ggcacccacg accgggtcca cctcccgggg cccgaacccg accatctccg tgatgtccgc
     7501 ccctcccgag aaccgccgct gccctcccca gaccaccacc gcgcggatcg cgtcgtcccc
     7561 cgccacctcc cgggacgaat cccacagctc ccgcgacatc cgctcgttca acacgttcgc
     7621 cggcggccgg tcgaggcgga tcgtggccac ggcgccgtcg gcctcgatgc gcacgaactc
     7681 acccatctcc tccgcccccg atccatcggt tcgccgccca tccttaccag accgggaccc
     7741 cgctgcggca gggagcacgg ccccgggggc gcctctgtcc accgaggccg gcctgcccga
     7801 ccccgcacgg cagactacgc ttccccggac atggagccga ccgcccggga agcgcaacgg
     7861 atcgagcggg tcctgaagaa gctccgggcc aggccggtcg gattcggacc cgtcaccacg
     7921 ggcagccaca ctcccgccag gcgctgggtg gtgacgctgg acgacggccg acgggcgttc
     7981 gtgaagatgg ccacggatga gctcaccgcg agctggctgc gggacgagca cctgctctac
     8041 tcgttgctga agggggcgcg attcatgccc gagtacctgg gctggtatga cgaccaggaa
     8101 cggccggtcc tggccatcga ggacctgacc gccgccgcat ggccaccacc gtggacgacc
     8161 gagcgggtgg aggccgttct ggcctgcctg gcggaggtca cggccacccc gcctcccgag
     8221 gggcctgccc cggggccgcg gacgacatct ggggattcgc gacggctggc gcgagatcga
     8281 ggcagagccc gagccgttcc tcgcgctggg tctgtgctcg ccctcgtggc tccggctggc
     8341 cctccccgat ctgagctccg ccgcgggatc ggcgccgctc ggcggcgagg ccctcctgca
     8401 cttcgacgtt cgcggggaca acctgtgctt ccgggacggt cgagccctcc tggtggactg
     8461 gaactgggcg tgcgtgggca acccgatggt ggacgtcgcg ttctggctcc ccagcctgcg
     8521 cgcggagggc ggaccccgtc ccgaggacgt gcctgcgagc ggggccgccg aactggcggc
     8581 cgcgtgcgcc ggcttcttcc gcgcccacgc ggcgcgtccc cccatcccca cggcgcccca
     8641 cgtccgtcct ctccagctca gacacgcccg cacggccctg ccgtgggcgg cccgggcgct
     8701 ggggttaccc tcgcccgctt gaacgaggat ttgcatctgc aaggtcttgc tattatggct
     8761 gggtgagcca gaggtcagcc gacgtggtag cgatcctcaa agaccacggg ctgcgcatga
     8821 cgccgcagcg gcgggccatc gtggcggcgg tcatgcggac caccggccac atctccccca
     8881 gcaccctggc caggagggtc caggcggaca tgcccggcgt gaacgcctcg acgatctatc
     8941 gcaccctggc gctcctggag gaggtagggg tgctggccca cgctcacctg gagggtgggg
     9001 ccgagtacca ccgcgcggag caaccccccc atgcgcatct ggcctgctcg cgctgcggtg
     9061 ccgagatcga gctgtcgctg gacgccaccc gggagctcga gcgcctgatc gtggagcggc
     9121 acgacttcgc gcccgacctc acccacttcg cgatctcggg tctgtgccgc gactgccgct
     9181 cacgcggctg aggcgaacga gaccctcccc ctcggtctag cccttcggag ccgctccggt
     9241 ctcggatccg ccggagccct cgtggtgccg gtagtcgtgc tgatgcaggt ggatgtcgag
     9301 cccgtgcgcg tggctgtgct cgtggaccag gttcacccga aggagcagct catgatcggc
     9361 gagcacccga ccgggcgccc cctcccccac cagtcggtgg tcctcagaga acaccaggca
     9421 tcgatcggcc atgatgtcga gcacgtccag gtcgtgggtg gccagcacga tcgtcttgcc
     9481 ggcccggttc agctccacga tcagctccag gagccactgc tgggtccgcg ggtccagcgc
     9541 cgcggtgggc tcgtcgaaca gcagcacctc ggggttcatc accagcaccg aggcgatcgc
     9601 gacccgcttc ttctcccctc ccgagagctg gaagggcgcc cgatcggcca gcccctggat
     9661 ccccagcatc tccatcacgt cggcgatgcg gcgctccacg cgcttgggag ccatccccat
     9721 ctgcagcggt ccgaaggcga tctcctcccg gaccgtgggc gagaagacct gggcgtccga
     9781 gttctggaag atgaacccca cccgggatcg gaaccccatg gagaactgct cgtcctccag
     9841 gttgtcctcg gtcacgagct gcccgaaggc ctcgtacgtg ccggagtcgg ggaacagcag
     9901 accgtccagg atcttcagca acgtggactt gcccgagccg ttggccccga gcagcgccac
     9961 cttctccccc cgacggatgg agagcgagac gccctccagc gccgggaagc gctccaggta
    10021 cgcgaaccgg agatcgcggc agaccatcag ctgctcgccc ttcggggcat cgagccggtc
    10081 ccgggtctgc gcgcggacct cagcgtccaa cgaggcggtc accccccacc agcagcaccg
    10141 ccagcatcag cgagatcagg gccacggcca ggtccacggc ccgcaccctg aacccctcga
    10201 gggtcttggc gtctccctgg tacccgcgcg agagcatcgc catgtgtacc tcctcggaga
    10261 gggcgtgcgc cttcccgaac agcgcccccg ccgtcgccgc gacgaaacgc tgaccctcct
    10321 tggcgttctc ccccttcccc accgagcgcg ccttgcgggc cgtgtacatg tcggtcaccg
    10381 agttcagcag gaggaacacg taccggtagg ccatgccgat gatcaggatg aagatccgcg
    10441 gcacgaacag ggcgcgcagc ccggccagca gcttcaccca gggtgtggtc agcgtcagca
    10501 ggacaaccag cgagacggag gtcgcgaccc gcatgacgat caggccggcc gatccgagcc
    10561 cctgtgccgt gatcccgacc tgatggccgc tccagtgcca caggggcagg acgatcgtcc
    10621 ccggcgtgat gaagctgaag gtggccggta gcaccacgat ccccgtgaag atcgggatga
    10681 acagccacac ccgcttgacg aagaacccga ccggcagggc ggagacggcc gccagcacca
    10741 gcgtgatgcc gtacatcacc agcagggcgg ggatgtggtg gaccagggcc gccgccagga
    10801 gcagcaggac ggtggccgcg atcttgatcc gggcatcgac ccgctggagc aacccatgcc
    10861 ggcgggcgat gtcgtccccg aacatggcct ggcgcatgat gttcgaggct ccgttcaggg
    10921 tcttctcgac gaagctgccc ttcgtgcggc ggccgatgca gccgcaggga cacagcccga
    10981 cctcggcctg caccaaccat cgtggggccg cgacgccccg gtcaccatcc tcgaccacgg
    11041 atgtcacgcc gcggcctctt ccacgtcccg ggtcgggcgc tcgggtcctc cgccgcgacg
    11101 gcgacgcagc agccgccaca ccaccacgaa accgagcatc acggttccga tcacgaggat
    11161 accgagcacg gccgacaaga tgtacgcgag cgccgcgtgg ttcccgctct cgaacccgta
    11221 tcccccgagc agggtatggc tccagaagct cgagaagcgc tccagcccgg cgggcaccgc
    11281 acgcaggttg taccgcccca ggtcgaggtt ggccggggca gcctcgccga aggcaccgcc
    11341 cggcgccagc aggccgagcg gcgtgagcac gatcatcacc cccatgccca ccagcaccca
    11401 tcgccacccg agcctcttcc ggcgctcgag ctcctcgtcg gtcaccggga ccttggggtg
    11461 gttcagccgc aggaccggca cgttggcccg ctgcaggtag ccgatgaccc cggccgccac
    11521 cacgaactcg gcgatgccgg ccaccgtgag gtgcgccagc atcatggcag ggatggtctg
    11581 ggccagatgg aagggcgcgt acaggggcgt gccgttcgcg gtgtggaaca gcgtgggctg
    11641 gaggccgaac tcgaccgccg cggccagggc ggccacgttg atccccgcat acccaccgac
    11701 ccccgcagcc agggcccgtt tgggagaggt cagcgacagg tgccggctga gcagccggta
    11761 cacggcgtag cccgcgaacg gcatcagcac ggccatgttg aaggcgttgg ccccgaacgc
    11821 cagcacgccg ccgtcgccga agaacagcgc ctggatcgcc agcgcgatgc tgacggcgat
    11881 caccgccgcc caggggccga ggacgacggc gatcatcacg gcccccaccg cgtgggcggt
    11941 ggtgccgtct gggaccggga tgttgaacat catcaccagg aagctgaacg cggccccgat
    12001 ggccaccagg ggcacgtagc ggctcttcac caccttccgc acgcggcgcg cggcctggta
    12061 ccagaacggc acggtggcgg cgcccagcgt ggcgcaggtc gccgggctga ggtagccgtc
    12121 cgggatgtgc accgttcctc ctccgatcgc cgaccgatcc gcatggaaat gcaactgcaa
    12181 tacaacgcag ctactaccat gcacggtacc gagggtggtg cgctccgtca aggctgccgg
    12241 ggcctcagaa cctcggcgtc tcgaggtgct cgccgaagac gtcgcgaagg gcggcgacga
    12301 tctcgccctc cgtgcacatc gctcgggcgc agtccaccag caggggcatg aggttccgct
    12361 cggttccggc ggcctcccgc aggcgggcca ggctcgcctc ggcgggctgg ctgcgccggg
    12421 acgcccgaac ctcccggagc cgatccacct gccgttgctc catgtcgggc tggatcacca
    12481 gggtggggat gggcgtctcg gagggctcta cgaagtccgt gacgcccacc ttgaccaggc
    12541 gtcccttctc gtagcgctcc tgctcccgcc aggcggattc ggcgatctcc tgctggaagt
    12601 agccactctc gatcccggcc aggaccccct ccagcatccc gcccgagccc atccgctcga
    12661 tccgggcgaa ctcctcttcc gccagctcct ccatccggcc ggtgagcgac tcgacgaagt
    12721 aggagccacc gagcggatcg atcacccgcg ggacacccgt ctcgtgggcg atgatctgct
    12781 gggtgcgcag ggcgagcttg gccgcatcct cggtgggcag ggccagcacc tcgtccaggg
    12841 cgttggtgtg gagcgactgg gtgcctccca gcacggcggc cagggcctcg atcgcggtgc
    12901 gcaccacgtt gttcatgggc tgctgggccg tgagcgagac ccccgcggtc tgggtgtgga
    12961 accgtaggcg gagcgacgca tcgtccctgg ccccgtagcg cccctgcagc cagcgggccc
    13021 agatccgccg ggcggcccgg tacttcgcga tctcctcgaa gaaatcgatg tgcgcgttga
    13081 agaagaacga cagccccggg gcgaaacggt cgacctccag gccgcgctgc cggccgagct
    13141 ccacgtaggc gaagccgtcc gccagcgtgt acgcgagctc ctgccacgcg ttcgcgccgg
    13201 cctcgcggat gtggtagccg gacacgctga tgggatggaa cttcggcacc tggtcggcgc
    13261 agaactccat caggtccccg atcaggcgca ggtgggggcg gggggggaag atccattcct
    13321 tctgcgcgat gtactccttg aggatgtccg tctgcagcgt cccgtacaac gccgaccaag
    13381 ggacgccctg gcgctccgcc gtggccagga agaacgcgaa caggatcacc gccggcccgc
    13441 tgatcgtcat cgacgtggtg atgtcaccga gcgggatgcc cgcgaacagg gtctcgaagt
    13501 ccgccaggga gtcggtggcc accccgcacc ggccgacctc gccctcgctc cggggatcgt
    13561 ccgagtcccg acccatcagg gtgggcatgt cgaaggccac cgacaggccg gtctgtcccg
    13621 cctccagcag gtacttgtac cgcgcgttcg tctcagccgg cgatccgaac ccggcgaact
    13681 ggcgcatcgt ccacagccga ccccggtaca tcgtgggata gaccccccgc gtgtagggga
    13741 agcgccccgg atagccgagg tcccggtccg ggtccaggcc atggacgtcg ctcgggtcgt
    13801 acagggggtc gagcgtctct cccgaaagcg tctggaagtc ggcatcgcgt tcgggcgagg
    13861 atcggtagtc gtcttcccag cggtcgcgtt cggccacgat gcctccagtt cggctccggt
    13921 tcccctaccc gcctccgccg cttgcccgtt ccaccaggat agcggcagcc cggtacggat
    13981 cggtccgacg ctcctcgagg tcctcggcga agccgggatc ggagccgatc aggtcggcga
    14041 tcgacgagcg gaggcgctcc gcggccaggc tctggacctc gcggagcagt cgggcccgtc
    14101 gcagggcccc gagccggtca ccgtcctcca ggtgggcccg atgcgcggcg atcgcctccc
    14161 acagctcctc gatcccgtcg ggggtcaccg ccgaggtctg gaccaccggt acgctccacg
    14221 gccgctccgg ccccatccgg atcatctgcc ggaggtcccg ggccgccgcc tcggccccct
    14281 cccggtcggc cttgttgacc acgaagacgt ccgcgatctc gagtaccccc gccttggcca
    14341 cctggatcgc gtccccccat ccgggcgaga ccaccacgag ggtcgtgtcg gtggccgccg
    14401 cgacgtcgac ctccgcctgg cccacaccca ccgtctccac gatgaccagc tcggtgcccg
    14461 aggcgtccag gatccggacg gcctccggag cggccagggc catccctccc agatggcccc
    14521 gggtggccat ggatcggatg aacacgccgg gatcggtggc gtgcgcctgc atccgcagtc
    14581 gatcacccag cagcgcgcca ccggtgaatg gggaggtggg atcgatggcg agcaccgcca
    14641 ctcggagccc gcgttctcgc cccgccgtga ccagctccgc cgccagcgtg gacttgccca
    14701 cccccggcgc cccggtcagg ccgagggtgt acgcgtgacc ggtccggccg aacagggcgg
    14761 cggagagctc ctcgagccct tcggccccgt cctccaccat ggagatggct cgggccaccg
    14821 cccggggatc gcccgccaga actccgtctg ccagctccac gagctccagc gtagccgcca
    14881 cggggcgccg gacgcggcac catcacacca ccgacagcag ccgcgagagc ctgctcagcg
    14941 tgctggggtg acggaccagc ccctggatgc ggatccggcc ccggatcacc cgggccagga
    15001 ccgtccggcc cccgcggcgc agcggatcgg gcagaccgaa ccgcagcggc acggatgcca
    15061 ggagcaggag atcctccgct ggcgcccgaa cgtgcacgtg ggagcggacg ctgggatggt
    15121 ccccgctcga cacgcggatc cgtccgggct cgatcgagag cgtcaccgag accgccgcgt
    15181 ccggcgccga cagatcgatc acggccgggc ggagcagccg cacccgttcc ggatgcctcg
    15241 ccaggttcgc ctcgatcaat cccccgacca tcgcggccag gccctggggc tctggctccg
    15301 cgtactccac cctcacggtc gcagaaggac cttcgtagct cgccgcgacg cgaacagctc
    15361 gtagccgagc ggcgcctcgt ccagctggag ccggtgggag accaccggca tcggatcgag
    15421 gtccccggcc agcacctggt cgcgagcccg atcccagtgc gcatgcacgg ggcagatccc
    15481 ggcgaagcgg acctccagcg cgcgcgtcca ccacaccccg gcctgcatcg gcatcgtttc
    15541 ggacgtgaac atgcccacca cggcgatccg ccctccccgc cggaccacgt ccagcgcgct
    15601 ggcgaacgcc tggggtgtcc ccaccgcctc gatcacgacg tcggcgccgc gaccacccgt
    15661 ccgttccgcc accgccgtgt gcgggtggtg agcgcggacg tcgatcgccg tggcaccgaa
    15721 gcgcgtggcg agcgccaagc gctcgggctc cagatcgagg gccagcagct cggacggacc
    15781 gtggctccga gccgcctgca cacagaagtg ccccaccggg cccacaccca ccacggccac
    15841 cgcgtccccc ggctcgatcc cggccacgga tgctccgtac acgccggtgg tgaggacgtc
    15901 ccccaggaac acggcgggct cgtccccgat ccggtcgggc agcgccagca ggttcacgtc
    15961 ggccgatggc acccgaacca gttccgcctg ggtcccgccg agctcgccgc cgaacaggcc
    16021 acccccgagc atccgccact cgtcgcacag ctgggtctgg cccctccgac agaaccagca
    16081 gtctccgcag gcgatgtgga aggccacggc cacgcgatcg ccgggacgga aacgctccac
    16141 ggcgctcccc accgactgga ccacgcccat ggcctcgtgc cccatcacgt cgccggggtc
    16201 cagcggtgcc ttcccatgca ggaaatgcag gtccgaggcg cagatcgcgc tgaccccgac
    16261 ccgcaccacg gcatctcggg agtcctcgac ctccggctcg ggcacctcct ccacggtcac
    16321 ccggccgatc ccgcggtaga cgacggccct caccggcggc cctcggtcat ccggcgctca
    16381 cgccgtctgg ggaggacgct cgtcgaggaa ctcggtgggc acgctcatgg tgaggtggac
    16441 gagctcgttc gcggcgcgaa cgtacctgat gatggtgggc aggtctccct ggagcttgag
    16501 cttgccctgc atcatcccct tgaccgggtc cagatcgccg gccaagacct ccttccaccg
    16561 gctgtacggg gctcggatca cgtagggcgc ccgctcaccc tcctcgggtg tcacgatccg
    16621 cccggctcga caggtgccgt gccataggtc gagccatccc cagatgtcct gagggacgtt
    16681 ccgatcgggc tcggcctcga agacgaaggc gatgtcccct tcccagtccg atgcggactc
    16741 ccggtagtcg gaggatccat tgatcctgtc cacgtagatc gcgaaccagg cgtcgctcgg
    16801 gaacatcgcc accccgctcc ccctcccctc gctccgccct cagcacctca tcgaagggcc
    16861 aggatcagga ccaggccgat cacgttgaag gtggcgtgcg ccaccatgtt cgcccccacg
    16921 ttgccccgcc gatcgtagag ccacgcgagc gcgatgcccg tgaacaccat gacggtcatc
    16981 agcagggcgc tgtcgtacca cgtggagggg atgtaatgga tcagcccgaa cgcgagcccc
    17041 gagcccacgg cccccacgcc gaaaccgtac cggtcgcgca gggagcggaa caggatgccc
    17101 cggaagaaga actcctcgtg gaacggggcg atgatcacgg cgtacacgat cgccaccgcg
    17161 acgcccagcg ccgacaggtg gggcggcacc tgctgaggcg cctccggctg gtgcccggac
    17221 acggccgaga gcaccaccgt gaccaggagc cccacgccga gcgtgatcac cgggtacagg
    17281 acgatgccga accccacgcc cgaccgcacc tcgggccata ggcgacgggg gaacccgatc
    17341 gcctgtcgcc actcccgatg gaagcgggag
//
LOCUS       DASQQQ010000059         2436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_197873, whole genome shotgun sequence.
ACCESSION   DASQQQ010000059 DASQQQ010000000
VERSION     DASQQQ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197873"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1689
                     /locus_tag="VE646_02615"
     CDS             226..1689
                     /locus_tag="VE646_02615"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HZD78921.1"
                     /translation="MKQQRGAPRSMPMQHGRQPMTAEHRREMVRGYHVRTLWIPLMLL
                     VVGVWLATAPFTFGYLGSGSAGPRVAEVTRLRGLANVGTRGLWMATNDLVAGLLLISF
                     ALLSLRPGAIWPRWAAAGVGAWLVFAPLFLWAPTSAAFLNDTLVGILVVALTILIPMM
                     PGMMLIMQPGPEVPPGWSYNPSAWLQRAPLIALSWTSFFFARYLAAYQLGYLDAAVDP
                     FFGDGTMWVLDSSVSLAFPISDAGLGAAAITIEALMGYMGGTSRWRTMPWMVTFFGIL
                     VIPLGVAHVVLVILQPVVVGSWCTFCLAAAIPMLIMIPLALDEVVAMVQFIRERLRRG
                     DAFWSVFFKGGTLDADGGDERTPGYPGSPGRLAAAAAWGVTVPWTLAVGALAGVWLMI
                     SPDVLGATGSVADANRIVGATAVVVAVVATAELARGLRFLNVVVGAAALVVPMLLGDA
                     PAGLWWTNVGVAAVLVASTLPRGAIRQSYGSADRFVR"
     gene            complement(1947..>2436)
                     /locus_tag="VE646_02620"
     CDS             complement(1947..>2436)
                     /locus_tag="VE646_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78922.1"
                     /translation="TVIRIGPSTLSQLAIELPGGAAPAGIAVGGGSVWVADKLGARIL
                     RIDPSKNEVVGRGTPVLGGQPDRVAFGAGFVWATVPSNDSVTRIDPETGEGVTIEHVG
                     NGPTGIAAGTEGVWVANGLDGTVAHIDPSGARVVGRVEVGFIPDGVAVGAGAVWVTIR
                     AT"
BASE COUNT          308 a          850 c          878 g          400 t
ORIGIN      
        1 cgcgtagatc cctcgcagat gcccggggga cgagccggac gagaaggcgt cctccaactc
       61 ggcgagcgcc ggcggccgat ccagagcgac cgtggcgacg gtgcgccaga acgcctccgg
      121 ctccatcgaa cgatgctgcc acaccgaccc atcgatgtcc ggaggttccc gcgcacggac
      181 ggtttccttc gagcggccga agggtacccg cgtcttggga tggacatgaa gcagcagcga
      241 ggggcgccgc ggtcgatgcc gatgcagcac gggcggcagc cgatgaccgc cgagcaccgc
      301 cgggagatgg ttcgcggcta ccacgtccgc acgttgtgga tccccctgat gctcctggtg
      361 gttggagtct ggctggccac ggcgccgttc acgttcgggt acctcgggtc tgggtcggcc
      421 gggccccgcg tggccgaggt cacgcggctg cgcgggttgg ccaacgtcgg gaccaggggc
      481 ctgtggatgg cgacgaacga cctggtcgcc ggactgctgc tgatctcgtt cgcgttgctc
      541 tcgctgcggc cgggcgccat ctggccacga tgggcggcgg cgggcgtcgg ggcgtggctg
      601 gtgttcgccc cgctgttcct gtgggcgccg acttccgccg ccttcctgaa cgacaccctg
      661 gtggggatcc tggtcgtcgc cctgacgatc ctgatcccga tgatgcccgg catgatgctg
      721 atcatgcagc ccgggcccga ggtgcccccg ggatggtcgt acaacccgtc ggcgtggctg
      781 caacgggcgc cgctgatcgc cctgtcgtgg acgagcttct tcttcgcccg gtacctcgcc
      841 gcctatcagc tcggctacct cgatgccgcc gtggacccgt tcttcggtga tggcacgatg
      901 tgggtcctcg attcgagcgt ctcgctcgcc ttcccgatct cggacgccgg gctgggggcg
      961 gcggcgatca cgatcgaagc cttgatgggc tacatgggcg gcacgtcgcg ctggcggacc
     1021 atgccctgga tggtgacgtt cttcgggatc ctggtgatcc cgctcggcgt cgcgcacgtg
     1081 gtcctggtga tcctccagcc ggtggtggtc gggagctggt gcacgttctg cctggcagcg
     1141 gcgatcccga tgctgatcat gatcccgctc gcgctggacg aggtggtcgc catggtgcag
     1201 ttcatccggg agcgactgcg gcggggcgac gcgttctgga gcgtgttctt caagggcggg
     1261 acgctcgacg cggacggcgg cgacgagcgg acccctgggt accccggttc gcccggccgg
     1321 ctcgcggcgg ccgctgcgtg gggcgtcacc gtgccctgga cgttggcggt cggggcgctc
     1381 gccggcgtgt ggctgatgat ctcgcccgac gtgctcgggg ccaccggatc ggtggcggac
     1441 gcgaaccgca tcgtcggtgc gacggcggtc gtggttgcgg tggtggccac ggccgagctc
     1501 gcccggggcc tccggttcct caacgtggtg gtcggggcgg cggccctcgt cgtgccgatg
     1561 ctgctgggcg atgcgccggc gggcctgtgg tggacgaacg tcggggtggc cgccgtgctc
     1621 gtcgcctcga ccctcccccg cggcgccatc cggcagtcct acggctccgc ggatcgcttc
     1681 gtccgctgac ggtgctccgc ggcgtatgac cggcacgggc accaccggcc cttgacgatc
     1741 agctggtgcc cggggtgggg cgccaacaga atccacacgt ctcggcggcg gccttgagct
     1801 gctggttcgt gtcccgtgga ccaaatcccg ccctcaacgt cgcgagcccc agggaacccg
     1861 gccgccggga aacctgagcc caaagggcga ggcgaggacc cggacagacc cgatggcggg
     1921 cagcaggcgg gctggaggcc cttcggtcag gtggcccgga tggtgaccca caccgctccg
     1981 gcacccaccg cgacgccgtc gggaataaac ccgacctcga ctctccccac caccctcgcc
     2041 ccgctgggat cgatatgggc gacggtcccg tcgagaccgt tggccaccca caccccctcg
     2101 gtcccggcgg cgatccccgt gggaccgttc ccgacgtgct cgatggtgac accctccccg
     2161 gtctccgggt cgatcctggt cacggagtcg ttactgggga ccgtggccca cacgaacccc
     2221 gcaccgaagg ccacccgatc cggctggcca cccagcaccg gtgttcctct gcccacgacc
     2281 tcgttcttcg agggatcgat ccgcaggatc ctcgcaccga gcttgtcggc gacccacacg
     2341 gatcccccgc cgacggcgat gccggcgggc gccgcgccgc cgggaagctc gatggccagc
     2401 tgggagagcg ttgaggggcc gatgcggatg accgtt
//
LOCUS       DASQQQ010000060         6317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_114434, whole genome shotgun sequence.
ACCESSION   DASQQQ010000060 DASQQQ010000000
VERSION     DASQQQ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114434"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="VE646_02625"
     CDS             complement(<1..1248)
                     /locus_tag="VE646_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HZD78923.1"
                     /translation="MLGPDGRIDALPSSNGSVKIGVYVCHCGLNIAGKVDVSAVVEFA
                     STLPGVVVARDYKFMCSDPGQELIEQDLRDGLVNRVVVASCSPLMHEATFRGATARGG
                     QSPFYFQMASIREHVSWVTADGDDATEKAKALVAGAVRRVALHEYLEPRKAEVRPDVM
                     IVGGGIAGIHAALTLADAGKHVYLVERETTIGGHMAKFDKTFPTLDCAACILTPKMSA
                     VRAHPNITLWTYSDVVAVEGFSGDFTVRVRHRPRYVVEDLCTGCLECIEKCIHKQGKF
                     EDEWNEGLSKRKPIYIPFPQATPPVVVVDPDTCIWFKTGKCPQTCIEACERDAFDFEQ
                     TETFDEVKVGAIILATGYKTFDAGRMPEYGYGLYPDVYTSLELERLLNAAGPTGGEIV
                     LRNGSKPKVVGIIHCVGSRDAKHN"
     gene            complement(1235..2221)
                     /locus_tag="VE646_02630"
     CDS             complement(1235..2221)
                     /locus_tag="VE646_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HZD78924.1"
                     /translation="MTRRRYTYYPGCSAEGVGRAFTESLYAVFTHLDAELEELEDWNC
                     CGATAWPSVDQDQAFALAARNLAIAERRAPGSEPVDLVASCTGCYRALIKAEEAMVTP
                     GERAERLGGALGAIGLRYEGRARTRHPVDILLNEIGVDRIRQAVVRPLTGVKVASYYG
                     CQLVRPFATFDDPRDPTSMDRLVEAIGAEPIEWPLKARCCGGSCYCGGPVIGAMPEAT
                     MQLSYALLREAGKRGADVIATICPLCQFNLEGFQGRMAKRFDHPLDMPVVFITQLLGI
                     ALGIDEKRLGVRRLLRWRLPEPAASPPAPRGRRQPAAVGAGAGEEGGADARA"
     gene            complement(2218..2841)
                     /locus_tag="VE646_02635"
     CDS             complement(2218..2841)
                     /locus_tag="VE646_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZD78925.1"
                     /translation="MQTNGTRRKGRGSRKRWRPVKDQSKFEAQLEPGFAAEIAAEVLD
                     GDRLHQCIQCGTCSSACPLSAYMDLTPRRLIAMTRAGARDDVLHSMTPWVCASCYACT
                     VECPKRIPITEIMHALRRISLREKTYPRRFTTPVMTREFVDMIQDRGRSTESWISMKL
                     YLRTNPALLVKNTLIAGRLFRQGRLGVRRGSIEARSELRTLLEAVSS"
     gene            complement(3013..3483)
                     /locus_tag="VE646_02640"
     CDS             complement(3013..3483)
                     /locus_tag="VE646_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HZD78926.1"
                     /translation="MIGCGNPDAGDDAVGLLAVRGARARLGSLRDVEVVEASTIDLVH
                     LLEDVEGVVVVDAVREPSGLREPGTIVRAEAGPAGLPTDLRSSLSSHGLGVGEAVGLA
                     VALGQRPQVIFLGVEAGDVTMGHGPSARVAGALPELVDRIVEETRRLTRGQSRP"
     gene            complement(3504..4796)
                     /locus_tag="VE646_02645"
     CDS             complement(3504..4796)
                     /locus_tag="VE646_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HZD78927.1"
                     /translation="MTRRSVEVPVIARVEGEGALHVKVDDGRITDLRLEIYEPPRFFE
                     AFLQGRHYSEVPDITARICGICPVAYQMSAVHGLERLFEVRVPPGTEELRRLLYCAEW
                     IESHVLHIFMLAAPDFMGFDSAIEMAKVYPDVVKAALRLKRIGNDLMAAIGGREIHPV
                     SPHVGGFSRAPRRRDLEAFLPRLEDAVAEMPGVSDVVAELPRPELPRVTELVSMVHPT
                     EYPMNLGDLASTTGRTFDAASFEEVSREVHVEHSNALHSVMVDTGMPYFVGPLARLNL
                     NHERLTPVARACMQRLGLQVPEFDPFSSMGARVVEVALAFEEALRLIRDYQPPEPPMA
                     EVRPRAGRATWVTEAPRGFLYHRYDVTEDGRVAAAKIVPPTSQNLRHMEQDLRGFLPG
                     VLDRPDEELTRLAEMVVRNYDPCISCATHFLRLEIERS"
     gene            complement(4793..5632)
                     /locus_tag="VE646_02650"
     CDS             complement(4793..5632)
                     /locus_tag="VE646_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfhydrogenase subunit delta"
                     /protein_id="HZD78928.1"
                     /translation="MSANGKPRIAVFKMASCDGCQLQLLDAEEALLEIAGAIDIVNFA
                     EASSHLEHGPYDVTLVEGSISTPEQIEQIRRIRADTGVLIAIGACATSGGVQALRNTR
                     DLGGFVRTVYPTPEYIDSLETSTPISDHVPVDLELTGCPIDRGQLLGALGSLLAGAMP
                     RVSTAPVCAECKRRGYVCVVVAKGEPCLGPVTRTGCGALCPGFGRGCYGCFGPSQPGN
                     DAALARRFLELGDRPDRVADRFRFITGWAPVFREAARKVEHGRSLHPAGAGPAGPKEE
                     VHP"
     gene            complement(5629..>6317)
                     /locus_tag="VE646_02655"
     CDS             complement(5629..>6317)
                     /locus_tag="VE646_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HZD78929.1"
                     /translation="IGEVPISISSDPGHPEHLGHTIRFVGRVTNAFKDLAPGDEIGIR
                     GPYGRPWPLEEAEGGDVVVVGGGVGICPVRPAIEFAMRHRDRYRRLILLMGARHPSLF
                     PYREELDRWMGQMERLQVEMHLAIDEPADGWLYDVGVVTTLFPKAQIEPARTTAFVCG
                     PEIMMRFAGRGLLQLGLRPEQLYVSMERNMQCGIKLCGHCQMGPYFTCTDGPVFRYDE
                     VADLMEVHDL"
BASE COUNT          967 a         2284 c         2126 g          940 t
ORIGIN      
        1 gttgtgcttg gcatcgcgtg agcccacgca gtggatgatc ccgacgacct tcggcttcga
       61 gccgttgcgg aggacgatct cgccaccggt gggtccggcg gcgttcagca ggcgctcgag
      121 ctccaggctc gtgtagacgt cggggtacaa gccgtagccg tactcgggca tcctgcccgc
      181 atcgaacgtc ttgtagccgg tcgcgaggat gatcgctccg accttgacct cgtcgaaggt
      241 ttccgtctgc tcgaagtcga acgcgtcgcg ctcacaggcc tcgatgcacg tctgcgggca
      301 cttgccggtc ttgaaccaga tgcacgtgtc gggatccacg acgaccaccg gcggggtggc
      361 ctgcgggaac gggatgtaga tcggcttacg cttggacagc ccctcgttcc actcgtcctc
      421 gaacttgccc tgcttgtgga tgcacttctc gatgcactcc aggcatcccg tgcacaggtc
      481 ctccaccacg tagcgagggc ggtggcggac ccgcacggtg aaatcgccgc tgaacccctc
      541 gaccgcgacc acgtccgagt acgtccacag cgtgatgttg gggtgcgctc ggacggcgga
      601 catcttcggc gtgaggatgc aggcggcaca gtcgagggta gggaacgtct tgtcgaactt
      661 ggccatgtgg ccgccgatcg tcgtctcgcg ctcgaccagg tacacgtgct tgccggcgtc
      721 cgcgagggtc aacgcggcgt ggatcccggc gatcccaccg cccacgatca tcacgtcggg
      781 gcggacctct gccttgcggg gctcgaggta ctcgtgaagg gcgacccggc ggacggcccc
      841 cgccaccagc gccttggcct tctcggtcgc gtcgtcgccg tcggccgtca cccaggaaac
      901 gtgctcccgg atgctcgcca tctggaagta gaaggggctc tgcccgcccc gggcggtggc
      961 gccccggaac gtggcctcgt gcatcagcgg cgagcaggat gccaccacca ctcggttcac
     1021 caggccgtcg cgcaggtctt gctcgatcag ctcctggccg ggatcggagc acatgaactt
     1081 gtagtccctg gccaccacca cgccgggcag ggtggaggcg aactccacca cggcggagac
     1141 gtcgaccttg ccggcgatgt tcagaccgca gtggcagacg tacacgccga tcttgacgct
     1201 cccgttggat gagggcaggg cgtcgatccg accatcaggc ccgagcatcg gcgcctcctt
     1261 cctctcccgc cccggcgcca accgccgcgg gctggcgtcg cccgcggggc gccggcgggg
     1321 acgccgccgg ctcggggagc cgccagcgca ggagccggcg gaccccgaga cgcttctcgt
     1381 cgatgcccag ggcgatcccc agcagctggg tgatgaagac gaccggcatg tcgaggggat
     1441 gatcgaaccg cttggccatc cgcccctgga acccctccag gttgaactgg cagagagggc
     1501 agatggtggc gatcacgtcc gcgccgcgct tgcccgcctc gcgaagcagg gcataggaca
     1561 gctgcatcgt ggcttccggc atcgcgccga tcacggggcc accgcagtaa caggagccgc
     1621 cgcagcatcg agccttgagc ggccactcga tcggctcggc cccgatggct tcgaccagcc
     1681 ggtccatcga tgttgggtcc cgggggtcgt cgaacgtggc gaaggggcgc accagctggc
     1741 agccgtagta cgaggcgacc ttgacgccgg tgagcggccg gaccaccgcc tgacggatgc
     1801 ggtccacccc gatctcgttc agcaggatgt ccacgggatg gcgcgtccgg gcccgaccct
     1861 cgtaccggag cccgatcgcc cccagcgccc cgcccagccg ctcggccctc tcgccggggg
     1921 tgaccatggc ctcctctgct ttgatcagcg cccggtagca gccggtgcac gacgcgacca
     1981 gatccacggg ttccgaaccc ggcgctcggc gctcggcgat ggccaggttc ctggcggcca
     2041 gcgcgaacgc ctgatcctga tccaccgagg gccaggccgt ggccccgcag cagttccagt
     2101 cctcgagctc ctccagctcg gcgtccaggt gggtgaagac cgcgtacagc gactcggtga
     2161 aggctcgacc cacaccctcc gcggagcacc ccgggtagta ggtgtagcgc ctgcgggtca
     2221 tgaggacacc gcctccagga gggtgcggag ctcgctccgt gcttcgatcg agccccgcct
     2281 cacgccgagc cgtccctggc ggaacagccg ccccgcgatc aaggtgttct tcaccagcag
     2341 ggccggattg gtgcgcaggt acagcttcat cgagatccag gactccgtgc tgcgcccgcg
     2401 gtcctggatc atgtcgacga actccctcgt catcacgggg gtggtgaacc gccgggggta
     2461 ggtcttctcc cggagcgaga tccgccgcag cgcgtgcatg atctcggtga tggggatccg
     2521 cttggggcac tcgacggtgc aggcgtagca cgacgcgcac acccacggcg tcatgctgtg
     2581 caggacgtcg tcccgggccc ccgccctcgt catcgcgatc agtcgccgag gcgtcagatc
     2641 catgtacgcg ctgagcgggc aggcggagct gcacgtcccg cactggatgc actggtgcag
     2701 ccggtcaccg tcgaggacct cagcggcgat ctcggccgcg aaccccggct ccagctgcgc
     2761 ctcgaacttc gactgatcct tgaccggtcg ccaccgcttc cgtgaacccc tccccttgcg
     2821 tcgcgttccg ttcgtctgca tggccctccc ttcgggagga tcgtccgatc tcactcccag
     2881 ggtgccgctg ggccagcgtg gcgacacgag cggttggtcc cacgtcggaa gggcctctcc
     2941 gccctgcgga tgggaccgat cccgaggatg tagcccggga tcgctgcacc gcaagactct
     3001 ccggggcgcc gctcagggac gcgactgacc tcgggtcagc cggcgtgtct cctcgacgat
     3061 ccggtcgacg agctccggta gtgccccggc cacgcgggcc gaggggccat gacccatcgt
     3121 cacgtcaccc gcctccaccc ccaggaagat cacctggggc cgttgaccga gggccacggc
     3181 cagccccacc gcctccccca ctcccagccc atgggacgac agcgaggacc ggagatcggt
     3241 cggcaggccc gccgggccgg cctcggcccg aacgatcgtc cccggctcgc ggaggccgct
     3301 cggctcgcgg accgcatcga ccaccaccac cccctcgacg tcctcgagca gatgtacgag
     3361 gtcgatcgtc gaggcttcca cgacctcgac gtcccggagc gacccgaggc gcgcccgcgc
     3421 gccccgcacg gcgagcaacc cgacggcgtc gtcgcccgcg tccgggttgc cgcaaccgat
     3481 cacacggatc cggcgtggat cgctcagctc cgctcgatct ccaggcgcag gaagtgggtc
     3541 gcgcacgaga tgcacggatc gtagttgcgg accaccatct cggcgaggcg cgtgagctcc
     3601 tcgtcgggac ggtccagcac cccggggagg aacccgcgca ggtcctgctc catgtgccgc
     3661 aggttctgcg aggtgggcgg cacgatcttc gccgcggcga cccggccgtc ctcggtcacg
     3721 tcgtagcggt ggtagaggaa cccccgtggg gcctcggtga cccaggtcgc ccggccggcg
     3781 cggggccgga cctcggccat cggcggttcg ggcggctggt agtcgcggat cagccggagg
     3841 gcctcctcga aggccaacgc cacctccacg accctcgccc ccatcgagga gaaggggtcg
     3901 aactcgggca cctgcagccc gagccgctgc atgcacgcac gcgccaccgg cgtcaggcgc
     3961 tcgtggttta ggttcagccg cgccagcggc cccacgaagt agggcatgcc cgtgtccacc
     4021 atcaccgagt gcagcgcgtt cgagtgctcg acgtgcacct ccctcgatac ctcttcgaac
     4081 gaggcggcgt cgaaggtccg cccggtggtg gaggcaaggt cgcccaggtt catcgggtac
     4141 tcggtcggat gcaccatgga cacgagctcg gtcacccgcg ggagctcggg acgagggagc
     4201 tcggccacca cgtcggagac gcccggcatc tccgccaccg cgtcctcgag ccgcggtagg
     4261 aacgcctcca ggtcccgccg gcgcggggcc ctggagaagc cccccacgtg tggggagacc
     4321 gggtggatct cgcgaccgcc gatcgcggcc atcagatcgt tgccgatccg cttcaatcga
     4381 agggcggcct tcaccacgtc cgggtagacc ttcgccatct cgatcgcgct gtcgaagccc
     4441 atgaagtcgg gcgcggccag catgaagatg tggagcacgt ggctctcgat ccactcggcg
     4501 cagtacagga gccggcgcag ctcctccgtg ccggggggca cccggacctc gaacagccgc
     4561 tccagcccgt ggaccgcgct catctggtac gcgaccgggc agatcccgca gatccgggcc
     4621 gtgatgtcgg ggacctcgga atagtgacgg ccctgcagga aggcctcgaa gaagcgcggg
     4681 ggctcgtaga tctcaagccg cagatcggtg atccggccgt cgtccacctt cacgtggagc
     4741 gcgccctcac cctccacccg ggcgatcacc ggcacctcca ccgagcgccg ggtcatgggt
     4801 gcacctcctc cttcgggccg gccgggccgg ctcccgcggg gtggagcgag cggccatgct
     4861 cgaccttccg ggcggcctcc cggaagacgg gcgcccagcc ggtgatgaat cggaaccgat
     4921 cggccacccg gtcgggccgg tcgcccagct ccaggaagcg cctggccagc gcggcatcgt
     4981 tgcccggctg cgacggcccg aagcatccgt agcagcctcg gccgaacccg gggcagaggg
     5041 cgccgcagcc cgtgcgggtg accggaccga ggcagggctc gcccttggcc accaccacgc
     5101 agacgtagcc ccgccgcttg cactcggcgc acaccggcgc cgtggagacc cgaggcatcg
     5161 ccccggccag cagcgagccc agcgcgccta ggagctggcc gcggtcgatg gggcatccgg
     5221 tgagctcgag gtccaccggc acgtggtccg agatgggcgt ggaggtctcc agcgagtcga
     5281 tgtactcggg cgtggggtac acggtccgga cgaacccacc caggtccctg gtgttgcgca
     5341 gggcctgcac gccacccgag gtcgcgcagg ctccgatcgc gatcaggacc cccgtgtccg
     5401 ctcggatccg gcggatctgc tcgatctgct ccggggtgga gatcgacccc tccacgagcg
     5461 tcacgtcgta ggggccgtgc tccaagtgcg acgaggcctc cgcgaagttc acgatgtcga
     5521 tggcgccggc gatctccagg agcgcctctt cggcgtccag cagctgcagc tggcatccgt
     5581 cgcaggaggc catcttgaac acggcgatgc ggggcttgcc gttcgcgctc acaggtcgtg
     5641 cacctccatg aggtcggcta cctcgtcgta tcggaacacc gggccatcgg tgcacgtgaa
     5701 ataaggaccc atctggcaat ggccgcagag cttgatgccg cactgcatgt tccgctccat
     5761 cgacacgtac agctgctccg ggcgcagccc gagctggagc agacccctgc cggcgaagcg
     5821 catcatgatc tccggaccgc agacgaaggc cgtcgtcctg gccggctcga tctgagcctt
     5881 ggggaacagc gtggtcacga cgcccacgtc gtacagccac ccgtcggccg gctcgtcgat
     5941 cgccaggtgc atctcgacct gcaggcgctc catctgaccc atccaccggt cgagctcctc
     6001 ccggtagggg aacaggctgg ggtggcgggc acccatcaac aggatcagcc gccggtagcg
     6061 atcccggtgg cgcatcgcga actcgatcgc cggacggacg gggcagatgc ccactccccc
     6121 gccgaccacc accacgtcgc cgccctcggc ctcctcgagc ggccagggcc gcccgtacgg
     6181 cccgcggatc ccgatctcgt ccccgggcgc caggtccttg aacgcgttcg tcacccggcc
     6241 cacgaaccgg atcgtgtggc ccaggtgctc ggggtggccg ggatccgagg agatggagat
     6301 cgggacctcg ccgatcc
//
LOCUS       DASQQQ010000061         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_142905, whole genome shotgun sequence.
ACCESSION   DASQQQ010000061 DASQQQ010000000
VERSION     DASQQQ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142905"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1706)
                     /locus_tag="VE646_02660"
     CDS             complement(<1..1706)
                     /locus_tag="VE646_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZD78930.1"
                     /translation="MRRPPLERLVALLGSMLLAFGAIVVRLTVLQVSQADAYQSKALD
                     QRLRTVTLTATRGRILDRSDEPLAISMPARDVYADPRYVDDVAGEAARIAPMLGMKVA
                     DVEQRLSAGGTFSWVARQVDLGTAQRLERLQLPGLGFLPSTKRSYPAGSLASQVLGFV
                     NVDGTGIAGMELEYQKELAGTPGERTQEIDPNGQPIVGGVDVERPPVPGSDLVLTIDR
                     DFQFQVQAALEDAVHRNLAVGGTVVVMDPRTGEIYAMATYPWFDPNDPTASREAAWRA
                     RAITDSYEPGSVNKVITASAAVQEHAVGLDQRLRVPWTMKVGDFTIHDSHQHPVEHMT
                     LGDIVAESSNIGAVEVARRLGERRLDAYIGRFGLGKATEVGFPGEASGTVLPLWEWTD
                     TSLATMAYGQGIAVTPLQMAAVYATVANGGEWVRPRLVRGWVGPSGSFRPARPSATRR
                     VISPATARTVTQMLAYTVDAGTGTAAQIPGYQVAGKTGTARIPDPAGGYYTNRSIASF
                     IGFLPASDPRVVIAAMIDRPATVYGGIAAAPLFQQVARYAIQRLGIAPGAPVPLPPHA
                     LPV"
     gene            complement(1703..2041)
                     /locus_tag="VE646_02665"
     CDS             complement(1703..2041)
                     /locus_tag="VE646_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78931.1"
                     /translation="MGFLVLCAVVLGPFLLGLVTLNALLAQSSFRVDDLHQRIEALTQ
                     EHLELTRRHASLSAPGRIAAWARRSGMRLPDDIRSLHVASAGPAAPAGEADPASARDP
                     SSSAVTGGRG"
     gene            complement(2197..>2493)
                     /gene="mraW"
                     /locus_tag="VE646_02670"
     CDS             complement(2197..>2493)
                     /gene="mraW"
                     /locus_tag="VE646_02670"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HZD78932.1"
                     /translation="PQPARRTFQALRIAVNRELEELTASLPRAVGALAPGGRVVVISY
                     HSLEDRIVKRTFLDDPRLEVLTRKPQRPSPEEVADNARARSARLRAAERGEKAA"
BASE COUNT          366 a          898 c          889 g          340 t
ORIGIN      
        1 accggcaggg cgtggggggg cagaggcacc ggcgcgccgg gagcgatgcc cagccgttgg
       61 atcgcgtagc gggcgacctg ctggaagaga ggggcggcgg cgatgcctcc gtagacggtc
      121 gccgggcgat cgatcatcgc cgcgatcacc acccgaggat cggaggcggg caggaagccg
      181 atgaaggaag cgatcgatcg gttcgtgtag tagccacccg ccggatcggg gatccgagcc
      241 gttccggtct tgccggccac ttggtacccg gggatctgcg cggcggtgcc cgttccggca
      301 tccaccgtgt aggcgagcat ctgggtcacg gtccgagcgg tggcgggcga gatcacccgc
      361 cgggtggccg aggggcgtgc cgggcggaac gacccgctgg gaccgaccca gccgcgcacc
      421 aggcgaggcc ggacccactc acctccgttg gccacggtcg cgtagacggc cgccatctgc
      481 agcggcgtga ccgcgatgcc ctgcccgtac gccatggtgg ccaagctggt gtcggtccat
      541 tcccacagcg gcagcaccgt ccccgaggcc tccccgggga agcccacctc ggtcgctttc
      601 cccaggccga acctgccgat gtaggcgtcc aggcgtcgct cccccaaccg gcgggccacc
      661 tccacggcac cgatgttgct cgattcggcc acgatgtcgc cgagcgtcat gtgctccacg
      721 ggatgctgat gcgagtcgtg gatcgtgaag tcccccacct tcatggtcca cgggacgcga
      781 agacgctgat ccagccccac cgcgtgctcc tgcacggccg ccgaggccgt gatcaccttg
      841 ttcacggaac cgggctcgta cgagtccgtg atcgcccggg cccgccaggc ggcctcgcga
      901 gacgccgtgg ggtcgttcgg atcgaaccag gggtacgtgg ccatcgcgta gatctcgccc
      961 gtgcgcggat ccatcaccac gaccgtcccg cccacggcga ggttccgatg caccgcatcc
     1021 tcgagcgccg cctgcacctg gaactggaag tcccgatcga tcgtgaggac aagatccgag
     1081 ccgggcacgg gcggtcgctc cacgtccacc cctccgacga tcggctggcc gttcggatcg
     1141 atctcctgcg tccgttctcc cggggtcccg gccagctcct tctgatactc cagctccatg
     1201 ccggcgatgc cggtgccgtc cacgttcacg aaccccagta cctgggaggc cagcgacccc
     1261 gccgggtacg accgcttggt cgagggcagg aaccccagtc cgggtagctg cagccgctcg
     1321 agccgctggg ccgtgcccag atcgacctgc ctggccaccc atgagaaggt gccccccgcg
     1381 gataggcgct gctcgacgtc ggcgaccttc atccccagca taggtgcgat ccgcgcggcc
     1441 tccccggcga catcgtccac gtagcgcgga tccgcgtaca cgtcccgagc cggcatcgag
     1501 atcgccagcg gctcatccga ccggtcgagg atccgacccc gggtggcggt cagcgtcacc
     1561 gtgcgcagcc gctggtccag ggccttgctc tggtaggcat ccgcctggct cacctgcagc
     1621 accgtgagcc ggaccacgat ggccccgaac gccaggagca tcgaaccgag cagcgcgacc
     1681 agccgctcga gcgggggacg cctcaccccc ggcctccggt gacggcgctc gaggagggat
     1741 cccgtgccga tgcagggtcc gcttccccgg ccggagccgc cgggcccgcg ctcgcaacgt
     1801 ggagggaacg gatgtcgtcg gggagacgca taccggagcg acgggcccac gcggcgatcc
     1861 ggccgggggc ggacagcgac gcgtgccggc gcgtgagctc gaggtgctcc tgggtgagcg
     1921 cctcgatccg ctgatggaga tcgtccacgc ggaaggagct ctgcgcgagc agcgcgttga
     1981 gggtgaccag tccgagcagg aacggtccca ggacgacggc gcacagcacc aggaagccca
     2041 cggtgcctcg ccgcgggcgg ctcctggggc gccggaccgg ctctggcgct ccctccggcc
     2101 gcggtcgcgg tgccggcggc ggggtcggct tggggacggg agctccgacg gaggggcgga
     2161 aagcgggctg cgagccgagc gccctggccg ggaggctcat gccgccttct ccccacgttc
     2221 ggcggcacgc aggcgagcgc tccgggcccg ggcgttgtcc gctacctcct ccggcgacgg
     2281 ccgctgcggc ttcctggtga ggacctccag ccgcggatca tcgaggaagg tccgcttcac
     2341 gatccggtcc tccagcgagt ggtacgagat cacgaccacg cggccaccgg gagccagggc
     2401 acccaccgcc cgaggcaggg aggcggtgag ctcctccagc tcccggttga ccgcgatgcg
     2461 gagcgcctgg aacgtccggc gtgcgggttg ggg
//
LOCUS       DASQQQ010000062         3121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_335579, whole genome shotgun sequence.
ACCESSION   DASQQQ010000062 DASQQQ010000000
VERSION     DASQQQ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335579"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="VE646_02675"
     CDS             <1..150
                     /locus_tag="VE646_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002549866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD78933.1"
                     /translation="LSTKLSILGVPIDSNFLLMTPYIVTIAVVAGVVRKARPPAADGK
                     PYIKE"
     gene            155..580
                     /locus_tag="VE646_02680"
     CDS             155..580
                     /locus_tag="VE646_02680"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZD78934.1"
                     /translation="MAADPRVRDVRERFERSTFHNWIGMRLDRVEPGAVDVALDLEAH
                     HMNLVGIAHGGLLATVADTATGLAMRSALEPRTTHVTTQLSITFLTPGTGGTVVARGR
                     VLRSGRRFGYAEADVVDGQGTLLARASATFAIRPEAPAQ"
     gene            complement(574..951)
                     /locus_tag="VE646_02685"
     CDS             complement(574..951)
                     /locus_tag="VE646_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HZD78935.1"
                     /translation="MQFLSEGWANELKGRLNADEEFKKSLGSKNATLLQVVGGGDGER
                     RFWLQIDNGTVDLQMGNLQSPDATITEDYETAVALAQGQLNPVSAFMSGKIKISGNMM
                     LLMQLQGPLTGLPRVMREMDVDY"
     gene            complement(1040..1693)
                     /locus_tag="VE646_02690"
     CDS             complement(1040..1693)
                     /locus_tag="VE646_02690"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZD78936.1"
                     /translation="MKEPRNLFLIRHGRSDESSDDLLETPRGLQWNPPLHAVGREQAE
                     LLARRLALTHPAPAAVYCSPLRRARETIAPYVERTGVEVLIEDDLMEAHIGDWENKPF
                     EEIVASDEEILHLVRTQRAIWHRAPGGETVAGFRSRVDAAIEGILRRHPDGEVLVVCH
                     GGVINAYVGPLLGIQHEMFFLPENTSVNTVQVDGASRSVQFLNDVLHLTDPQFFLET"
     gene            complement(1690..2031)
                     /locus_tag="VE646_02695"
     CDS             complement(1690..2031)
                     /locus_tag="VE646_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78937.1"
                     /translation="MRSRAVHAAIKPSADGWTGRIPATGFVVTAPSPTRCLAALRKQA
                     GPDATLTVEVTPRLVGVAEAAAILGWDKRRVITYVDRGSFPDPLARLASGRVWRREDV
                     ESFARDRARRA"
     gene            complement(2096..2290)
                     /locus_tag="VE646_02700"
     CDS             complement(2096..2290)
                     /locus_tag="VE646_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78938.1"
                     /translation="MWALVPGVEVRRVAGDKPYRCPGCDHPIRPGLWHLVVVPEGAVE
                     ERRHWHEGCWRTELRRTGRA"
     gene            complement(2400..3077)
                     /locus_tag="VE646_02705"
     CDS             complement(2400..3077)
                     /locus_tag="VE646_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HZD78939.1"
                     /translation="MATPEIRGELSVRGATVGTAWIAPPGARGTLVVAHGAGVGMDHP
                     FLTGFCRAMGEEAVATLRFNFPYMERGRRSPDPESLLREAWLAAFDRAAERTGGPVLA
                     GGKSLGGRIASMCVADGMPAAGLVFLGYPLHPPGKPERIRDEHLYRITVPMLFLEGTA
                     DPFARPEVLGPVLGKLGDLAEHHAIEGGDHSFRVRGDRRDDREIGAALAGQAAAFVRR
                     VIRASTR"
BASE COUNT          452 a         1125 c         1084 g          460 t
ORIGIN      
        1 ctgtccacga aactgtcgat cctgggggtc cccatcgact cgaacttcct gctgatgacc
       61 ccgtacatcg tcaccatcgc ggtcgtggcc ggcgtggtcc gcaaggcccg gccgccggcg
      121 gccgacggca agccctacat caaggagtag gcgcgtggcc gccgatcctc gggtccggga
      181 cgttcgggag cgcttcgagc ggtcgacctt ccacaattgg atcgggatgc gtctggaccg
      241 cgtggagcct ggagcggtgg acgtggccct ggacctcgag gcgcaccaca tgaacctggt
      301 ggggatcgcc cacggggggc tgctggcgac cgtggcggac acggccaccg ggctcgccat
      361 gcgctccgcc ctggagccca ggaccaccca cgtcaccacc cagctgtcga tcaccttcct
      421 gacgcccggg accggtggga cggtcgtggc ccgggggcgg gtgctgaggt caggacggcg
      481 gttcggctac gcggaagccg atgtcgtcga cggccaggga accttgctgg ctcgggcctc
      541 cgccaccttc gcgatcaggc ccgaggcgcc cgctcagtag tcgacgtcca tctcccgcat
      601 cacccggggc agcccggtga gcggaccctg gagctgcatc agcagcatca tgttgccgga
      661 gatcttgatc ttgccgctca tgaaggccga gaccggattc agctgtccct gggccagggc
      721 caccgcggtc tcgtagtcct cggtgatggt ggcgtcgggg ctctggaggt tgcccatctg
      781 gagatccacg gtgccgttgt cgatctggag ccagaaccgg cgctcgccgt cgccgccgcc
      841 gaccacctgg aggagcgtgg cgttcttcga gcccaggctc ttcttgaact cctcgtcggc
      901 gttcagccgt cccttcagct cgttcgccca cccctccgat aggaactgca ccggcacggg
      961 agcctcccct ctgcgcgttc gcatcgtcac ctacgcctgc cggggcagta tatcggggcc
     1021 ccggacgcca cctggatcgt caggtctcca ggaagaactg ggggtcggtg aggtggagca
     1081 cgtcgttcag gaactgcacg ctccgggacg caccgtcgac ctggacggtg ttcaccgacg
     1141 tgttctccgg gaggaagaac atctcgtgct ggatacccag cagcggaccg acgtacgcgt
     1201 tgatgacgcc gccgtggcag acgaccagga cctcgccgtc gggatggcgc cgcaggatac
     1261 cctcgatcgc cgcgtccacc ctggaccgga accccgcgac ggtctcccca ccgggcgccc
     1321 ggtgccagat ggcccgctgg gtccggacca ggtggaggat ctcctcatcc gaggccacga
     1381 tctcctcgaa cggcttgttc tcccaatccc cgatgtgcgc ctccatcagg tcgtcctcga
     1441 tcaggacctc cacgccggtg cgctccacgt acggggcgat ggtctccctg gcacggcgga
     1501 gcggcgagca gtagacggcg gcaggcgccg gatgggtcag cgccaggcgc cgagccaaga
     1561 gctcggcctg ctcccgcccc acggcatgca gcggcgggtt ccactgcagg cccctgggcg
     1621 tctccaacag gtcgtccgag gattcgtcgg agcgaccgtg ccggatcagg aagaggttgc
     1681 gcggctcctt catgctcgtc gggcccggtc ccgggcgaac gactcgacgt cctcgcgacg
     1741 ccacaccctg cccgaggcga ggcgggccag cgggtcgggg aaggaaccgc gatcgacgta
     1801 cgtgatcacc cggcgcttgt cccagcccag gatcgccgcg gcctctgcca cgcccaccaa
     1861 tcggggcgtg acctcgaccg tgagggtcgc atcgggcccc gcctgcttcc gcagcgccgc
     1921 caggcagcgg gtgggcgacg gagcggtcac cacgaacccg gtggccggga tccggccggt
     1981 ccagccgtcc gccgacggct tgatcgccgc gtggaccgct cgagagcgca ccggccgatg
     2041 atagcgatgc cgtcgccggc cgcggcgtgg gtcgcggtct ggcacggttg gtggctcagg
     2101 cacggccggt ccgtcggagc tcggtacgcc aacacccctc gtgccagtgc cggcgctcct
     2161 ccaccgcgcc ctccgggaca accaccaggt gccagagccc cggccggatg gggtgatcgc
     2221 atccggggca gcggtagggc ttgtccccgg ccacccgacg gacctcgacg cccggaacga
     2281 gtgcccacat cggggcggcg gatcgcggac gatggaccga ttcgagctgc tccggcgctc
     2341 gccggtgacg gcgaggcatc aggccgctcc gggaaccgtg gggatcgcgg gccgatgcat
     2401 caccgcgtgc tcgcccggat cacgcggcgg acgaaagcgg cggcctggcc cgcgagcgcg
     2461 gccccgatct cgcggtcgtc gcgccggtcc ccccgcaccc ggaacgagtg atcgccgccc
     2521 tcgatcgcgt ggtgctcggc caggtcgccg agcttcccga ggaccgggcc cagcacctcg
     2581 gggcgcgcga aaggatcggc ggttccctcc aggaacagca tggggacggt gatgcggtag
     2641 aggtgctcgt cgcggatccg ctcgggcttg cccggtgggt gcagggggta tccgaggaac
     2701 accaggccgg ccgccggcat gccgtcggcc acgcacatcg acgcgatcct cccgccgagc
     2761 gacttgccgc ccgccagcac cgggcccccg gtccgttcgg cggcccgatc gaaggccgcc
     2821 agccaggcct ctctgagcag cgactccgga tcgggtgacc gccgtccccg ttccatgtag
     2881 gggaagttga agcggagcgt ggccaccgcc tcctcgccca tggcacggca gaagccggtg
     2941 aggaagggat gatccatccc tacgcccgcg ccgtgggcca ccaccagggt gcccctcgca
     3001 cccggtggag cgatccacgc ggtgccgacc gtcgcgcccc gcacggagag ctcacctcgg
     3061 atctcgggcg tcgccatctc tgcgatgatt ctgcccgatg gtggggacgc tgtacctggg
     3121 c
//
LOCUS       DASQQQ010000063        22958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_87354, whole genome shotgun sequence.
ACCESSION   DASQQQ010000063 DASQQQ010000000
VERSION     DASQQQ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 22958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22958
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87354"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(336..902)
                     /locus_tag="VE646_02710"
     CDS             complement(336..902)
                     /locus_tag="VE646_02710"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HZD78940.1"
                     /translation="MPRRVSFRPALSLRGRPSMGLRGFAGKPFHPPLTDIPIGAYVLA
                     AAFDLVSWIWASQEWSRDFYRAATFALIGGAVVSLGTALTGFLDWYRSAPKGTQARRT
                     ASAHGLTMATVTVLVLADIGIRLASYSEPSVPAGIAVLTIVVAAMTALGATIGGSLVY
                     DYGFNVQTARDSAVWHRSEQDVMPGQKG"
     gene            complement(966..1346)
                     /locus_tag="VE646_02715"
     CDS             complement(966..1346)
                     /locus_tag="VE646_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016557908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZD78941.1"
                     /translation="MSPVSVRYIVNDVDEAIGFYRDLLGFEVGMHPAPTFAILARGDL
                     RLLLSSPSGQGGGGQRMADGTSPEPGGWNRISLEVPDLPAEIERLSGLGARFRNELVR
                     GVGGDQVLLMDPSGNLVELFQYTS"
     gene            complement(1417..3345)
                     /locus_tag="VE646_02720"
     CDS             complement(1417..3345)
                     /locus_tag="VE646_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD78942.1"
                     /translation="MMGGRGFAHPGMMTPAARSKDFRGSTLRLAGRLRPERNKLLLVI
                     AFGIASVIFTIVGPKILGNAANVLFEGVISRQLPAGVSQQQVIAGLQARGQTQMAEML
                     SGMHLTPGAGIDFARIEGILLTLAGVYLLSAAFGWLQQYLMAGVAQRTMFRLREDVDR
                     KLARLPLRYYDDHPRGDLLSRVTNDIDNISTTLQQSLTQVVTAVLTIVGVLVLMVTIS
                     PVLALISLVAVPVSFVVTVTIAKRSQKQFAAQWERTGDVDGYIEEMFTGHEIVKVFGK
                     QPRAIASFDQQNEDLYQASFRAQFISGIIQPSMMFISNLNYVAIAVIGGIRVTNGTLS
                     LGDVVAYIQYARQFTQPIAQTASIMNMLQSAVASAERVFELLDEAEEEPDPVRPEVLA
                     TTEGHIGLRDVSFRYLRDAPLIERLSLEVPAGETIAIVGPTGAGKTTLVNLLLRFYEI
                     DSGRISIDGVPIRGMSRSGLRRLFGMVLQDPWLFHGTIRENIAYGREGASDGEIEAAA
                     GAARVDHFVHTLPDGYETVIDDEASNVSQGEKQLLTIARAFLADPQILILDEATSSVD
                     TRTEVLIQEAMSELMRGRTSFVIAHRLSTIRGARTILVMNHGAIIEQGDHEELLARRG
                     FYHDLYQSQFAEAFEPVP"
     gene            complement(3378..5108)
                     /locus_tag="VE646_02725"
     CDS             complement(3378..5108)
                     /locus_tag="VE646_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD78943.1"
                     /translation="MRRLLIAHLRPYRRQLVIVVTLLLAQAVANLYLPTLNADIIDNG
                     IAKGDTHYIVSIGGLMLGFTLLMGVAAVIAVYWAARTAMAFGRDVRGALFRRVESFSQ
                     MEVNRFGTPSLITRTTNDVQQVQMLVMIALTVMILAPIMAVGGIVMALRLNPRLSLLL
                     VVILPLMAAVVGFIVTRAVPMFRQLQVKLDRINLVMREKLAGVRVIRAFDRTRFEEQR
                     YGVANADLTDTSLRVMRLFALLMPAIMLILNLSTVAVMWFGSFQVGEGRMPIGDLTAF
                     LTYLMQILFAVLMATIMFVMVPRAAASADRIQEVLEVEPTVIDPAVPVPSPEHLGTLE
                     FRDVTFRYPGADEPVLSGISFAAGPGETTAIVGSTGSGKSTLVNLIPRLYDVTDGAVL
                     VDGVDVRELAQQDLWSRIGLVPQRAFLFAGSVGSNVRDGHVDASDEEVWHALTVAQGA
                     DFVREMPEGLDGPIAQGGANVSGGQRQRLAMARAVVKRPQIYVFDDSFSALDFATDAR
                     LRTALRAETRDTTVVIVAQRVGTIMHADRIVVLAEGRVAGIGTHQELLETCETYRQIV
                     FSQLSPEEVA"
     gene            5215..5673
                     /locus_tag="VE646_02730"
     CDS             5215..5673
                     /locus_tag="VE646_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZD78944.1"
                     /translation="MGGSARTEALAAVENAFSRFMARSSLPRLRQRVTEAVGAGVDFS
                     AFPTLARVASWGPLRTSELAERMGLDVSTVSRRIADLEQAGLVDRAPDPDDRRAHLLQ
                     ITGAGTRLVERMRQVRSGLLEEALGGWSTEQIQELADVLGRFTSALAELS"
     gene            complement(5713..6129)
                     /locus_tag="VE646_02735"
     CDS             complement(5713..6129)
                     /locus_tag="VE646_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78945.1"
                     /translation="MLKFRTMAPVTGPFVGGDNPVRGLAGGGFAWKLDRAHGKLRSDG
                     HLTVAVRGLVLVDDDPVPEPLRGTNPIPAFQAVVSCLTIDHGTAATTNVATDPFPAST
                     SGDSTIEATLDLPSPCIAPVVFVGPSPTVWFAATGA"
     gene            complement(6274..6690)
                     /locus_tag="VE646_02740"
     CDS             complement(6274..6690)
                     /locus_tag="VE646_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HZD78946.1"
                     /translation="MSRDQRRPPRRHEPSTFRCGHCRLDVSTRAVGTAHRNHCPSCLW
                     SRHLDDTPGDRTAACGSLMEPIAIAVRGDGEWVIVHRCTGCDEVHLNRAAGDDNPLIL
                     LRLAVRPLAQPPFPIELIGRLWSGSGTTRSRMPARP"
     gene            6997..7929
                     /locus_tag="VE646_02745"
     CDS             6997..7929
                     /locus_tag="VE646_02745"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HZD78947.1"
                     /translation="MVKRTASIAVGAILLWVVVVPAIPASAITTGRPDAGEHPYVGEL
                     IFYDADYVDPRFDEPGGWFSCSATMLSPDVIVTAGHCTYGVGRDGESTTSGSGNGSGG
                     NDIWFDNNENADFEGFPPNSDYGPDQNPQRYQDRARFLNRSPYWHRGTAYPHPRFADA
                     PFYVYDAGVVVLDQRLRVGDYGRIPTLDYLDRYQGAPHHLLEVVGYGLTASGPFTEEG
                     GDVRMKGDVKIDTLNSAPKDTFILLSNNKGKPHRGGTCFGDSGGPTFDGTDSNLVVAV
                     TSFGFSSTCSGVGGAYRIDQPDDQRFLARFGIRP"
     gene            complement(8011..8541)
                     /locus_tag="VE646_02750"
     CDS             complement(8011..8541)
                     /locus_tag="VE646_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78948.1"
                     /translation="MLATSTFMLVFRFFHIAAGVLWVGSAFLFVGFIGPSAAEVGPSA
                     VPLLTAAVKKRKVARVITGLGIVNVLAGWTMWLHDMGLYGSVGHWVSSRFGLVITIGA
                     VLATTSAFVGSIGVGANVERLVDLSGEIAASGAPPTTNQEQALNRISSALERHGKLDL
                     VLLILAVIAMATARYW"
     gene            8713..10014
                     /locus_tag="VE646_02755"
     CDS             8713..10014
                     /locus_tag="VE646_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HZD78949.1"
                     /translation="MTDHRTIVFFPEPGAWGPTNNCVAIAEVLRERGHRVVFVADASF
                     EGVLEGKGFEERVMRMAAPDENAHPTADPWAEFIRVTAPEFRKPTIEQQATVTRPIWE
                     ALVAGARYSHQRVMEIWADVRPDVVCTDNVTGFPAVELAGCPWARFVSANPLEMRDPD
                     LPPPLSGLPVADRSGWQAFVDEYHRVHEDLLAEHNGFRSSFGVAPCPSDEFNTHSPWL
                     NLYLFPEVADYRRARPLDRTWHRLQSTVRRGEAPFDVNEHVPGDGKVVYLSLGSLGCM
                     DVGLMQRLIDALGTTEHRVIVSLGPLGEQMTLGPRMYGESFLPQPSILPQCDLLITHG
                     GNNTTCEGFHFGLPMIGLPLFWDQYDNAQRLQETGFGLRLPTYDWEEGELVGAVERLL
                     ADQDLTERMRSNAEAIRSDPGAVRAADLLERLAVTGEPVVD"
     gene            complement(10030..10593)
                     /locus_tag="VE646_02760"
     CDS             complement(10030..10593)
                     /locus_tag="VE646_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZD78950.1"
                     /translation="MRFHPPQELRGELVRLRTYRPSDLDEMKASITASHEHLRGWMPW
                     AATPPTDGSVLEFLTPSIEAFGGTKAANYAITLAEDGTYVGGCGLMPRIGEGALEIGY
                     WVDARHTGRGIATESARLLTEAALGLNGVDQVEIHCDEANVRSAAIPRRLGYRLDRIE
                     PDQVKAPLETGRSMIWVAERDRWPVSG"
     gene            complement(10665..13415)
                     /locus_tag="VE646_02765"
     CDS             complement(10665..13415)
                     /locus_tag="VE646_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZD78951.1"
                     /translation="MVGRDAELALLNQTLQRVISEGRPALVTIVGSAGLGKSRLVAEL
                     LEVVGSAPEVYYWRSGRCLPYGAVSYSALADALKSQCEILEDDPTEVVQAKVEAAAVE
                     LLGGTELAPHLAALVGTGTEVALRREELFDAWRRFLDAMAARHPLVLAIEDLHWADPG
                     LLDFLEHLADWAEGPVLILGTSRPELLEIRPTWGGGKRNVTSIFLEPLRSDEDRAMLQ
                     DLLGGQLSAGIEDAIVERSEGNPLFIEEIVRSLIDRGVLRATEASRWEVALPVEEVAL
                     PRSIGALIAARLDTLPAEEKKLLQHASVVGRIFWSGALERLSGWNQDQAREVLGRLRV
                     KELVVPREPSTFSGELELGFRHVLIRDGAYESLPKAARVEQHLATARWAAKRVGDRGE
                     ELAELIASHYRQALRYLAELGRPVDDHPGLAMEALAWLRSAGDRALRLWQRSSALGWY
                     RDALELSDLAALEPFELLRLRTAYATSLHGVETWVVTREAFTRVRELADALGDDASAG
                     KAECELSLIAYVRGVLDEAIELAEEAVRRLEPLGEGEALARALDYLGRIRWRCGQNDE
                     AEAILHRSIEVAERVGALEVVADATITLGSVVAATDRDEGIGLLERGVGLAKRTGDFD
                     LVLRSENNLASILADFAGDFHRAEGIYADALETARKAGDRSWQTFLLGNLGDLARDYS
                     GDLPRSEGYLTESLELARAIGEELTIAMRMPSLGSARLLRGEVDEAEELYQESVRLSE
                     ASPEPQTFWWQKMFGGLTAAARGDADAALERSAESCRLVVRDASLVLLPLPLCERVRI
                     LLAFDRAEQAHEEAASLGDPDRFPPHMRAFVRITQGLLAADPAEQVSELREAATALAS
                     IGMRIHLGRCLIELGRAERRLGVDPRWTLERAREILAACRCGLYLREVEVDIGCLTSP
                     TG"
     gene            complement(13509..13901)
                     /locus_tag="VE646_02770"
                     /pseudo
     CDS             complement(13509..13901)
                     /locus_tag="VE646_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
     gene            complement(13920..14117)
                     /locus_tag="VE646_02775"
     CDS             complement(13920..14117)
                     /locus_tag="VE646_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HZD78952.1"
                     /translation="MAVCPSCGEDNPERARFCLSCGKALGSQAGPRMERKFATALFAD
                     LVGSTALAERTDPEVVRSVIA"
     gene            complement(14175..14960)
                     /locus_tag="VE646_02780"
     CDS             complement(14175..14960)
                     /locus_tag="VE646_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78953.1"
                     /translation="MTGPIDWEDEMRVPWLDRDTADRLLDGHVDPDDAPPGYQEVAGL
                     MRDVGAPASARELLHQDRAVSAASGIRSALGPVPVTPTPRRSTVRPKHFRAKLTGILV
                     IGTLVGTTGLAAAGVLPDPAQNVVSDALSQVGITIPSIEHPASTGTDISQMATSTDAT
                     GVAKGAQISDAASGGMSQAGQHGATSQARPSEAPPVTTPNPGGTATANAASGGAAEVG
                     TSIADQESQGRSAEGSGNAGRTGAAPTPASQPTPTSLPLPAAR"
     gene            complement(14957..15514)
                     /locus_tag="VE646_02785"
     CDS             complement(14957..15514)
                     /locus_tag="VE646_02785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZD78954.1"
                     /translation="MSLGERFEETLRSARSGAGWALADLYRDVHPRVLRYLRALDGAE
                     AEDLASEVWLDAIDGLDRFEGDERAFRAWMFTIARRRWIDLRRRRARRGTEPVSPERL
                     ATHARIGNVEDEALAALATEEALSRIASLPSDQAEVLLLRALAGLEVRQVARIMGKRP
                     GTVRVLQHRALRRLARTMAEQGVTR"
     gene            complement(15582..16472)
                     /locus_tag="VE646_02790"
     CDS             complement(15582..16472)
                     /locus_tag="VE646_02790"
                     /inference="COORDINATES: protein motif:HMM:NF028234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HZD78955.1"
                     /translation="MTVWAGLSVAFLGGDAREVEMAALAVAEGAAVRRFGTPEVDVGR
                     PSGGLVDSLAEALSGARVAVGPIPLPGPDGRIFAPSAAEPIYLTAEVLRRMALSAHVV
                     IGKADPGLRQAAADCGVRVHEYEHDTELMLLRTPAIAEGAIAVAIQRSPVTIHGSPIA
                     VIGFGRTARILTRTLLALGARVHVVARRPEARAEAIAMGAQATDLAGIADLFRGMAIV
                     FNTVPARVLSRGALGRLPRGALVVDMSAPPGGVDGEVATELGLDHVWARGLGASAPRT
                     VAASQWAGVSRIVAAALAQD"
     gene            complement(16469..17578)
                     /locus_tag="VE646_02795"
     CDS             complement(16469..17578)
                     /locus_tag="VE646_02795"
                     /inference="COORDINATES: protein motif:HMM:NF014381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HZD78956.1"
                     /translation="MSGTATGTVAVAVLGAGHGGLAAAADLARRGFHVRLWNRGPEPV
                     ERVTEAGGIRSSGAIGEGLVRIDTATTDLGRAIAGADVVVIVLPAPAHGEVFAALAST
                     MLGELPIVLDPGHMCGSLHLRRMFEIAGRPVPPCAELGTLSYICRSSAPASVQVSLRT
                     REVPMAVSGRIRPEVRALTADLFPGTRAAGVVETWLHDVNLVLHPPGMLLGAAWIEGT
                     DGGFGFYTQGVTPSVERTMLALDMERRAVGRAFGLDLPALTDTMAAYGSADLDRARAG
                     DLRGAIRGGAANAGIQAPTSVDHRYLHEDVGFGLVPLVALARIAGIPTPVAEALIVLA
                     ETITARPYRREGLTAEVLGIAGMDVEAFTHHLEHL"
     gene            complement(17608..18762)
                     /locus_tag="VE646_02800"
     CDS             complement(17608..18762)
                     /locus_tag="VE646_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008846982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZD78957.1"
                     /translation="MKLTSIQTFIVDLPKRRAHNWAGKMSTPIGSHLLVRLGDDEGHV
                     GWGEAPAIPTWGGPHMRYSGEAPNTAELMIRDHLWPAIEGRSPLEPAVLHGAMDSVVK
                     GNPYAKAALDIACFDLTGRALGVPVSTLLGGRVRDRVEVCHSLGIMDDDPALDEAEGA
                     VAEGIGTIKVKTGVDPERDVRLVRRLREHLGDDVRIRVDANEGYASVAEAVGVTRRQV
                     AEAGIFLCEQPMRGARALAEVARRIDVPVMADESAWTPQDVIELAELHAAEVISLYVA
                     KPGGLHRAREQAVVAEAFGMTSDIGGSIEMGIGNAANLQLGAATPIAVLPSVCPVSSP
                     KEAGPGIAGNYYTDDVIAEPLRFERGALLVPEGPGLGIEVDEEKVRAYAR"
     gene            complement(18759..19307)
                     /locus_tag="VE646_02805"
     CDS             complement(18759..19307)
                     /locus_tag="VE646_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZD78958.1"
                     /translation="MPVVTVQLWEGRTLDQKRALVAAITKAMVDHADADPSGLHVVLQ
                     ESTPQNWGRAGVLGVDREPGPPRPARVFGLHHLLLQVSDLARAEAFYVNGLGFVVRKR
                     ATLADGRDLTVTEQGLGLTQGGPEPPGPVEHIAFRVSDVEAVAERVRGAGGRIVKGPG
                     PGPYGTTLYFLDPDGNKIELHQ"
     gene            complement(19307..19993)
                     /locus_tag="VE646_02810"
     CDS             complement(19307..19993)
                     /locus_tag="VE646_02810"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HZD78959.1"
                     /translation="MVQALRNQRLSRGVVDGLLAAVRAGEFAPGDRLPSERRLAEAFG
                     VSRPSIREGLRILELIGAVEVRQGRGAIVLEDATEPSGTLLRGWLRSHQDEVLDLLDV
                     REVLEAAAAASAASRSRAEAEPITAVADDEDLEARVKADVAFHGLIADRSGNAILAGL
                     IHELNGVLETSRFAMFAMPGRPERSLEDHRRIAKAVNSGDPVGAARAMRRHIERTRKE
                     IEMLDAKGTG"
     gene            20054..22279
                     /locus_tag="VE646_02815"
     CDS             20054..22279
                     /locus_tag="VE646_02815"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZD78960.1"
                     /translation="MAGSPEDVADAKRLLTGRGRFVADVAVPGAVSMTVVRSPIAHGR
                     LRATSVDRARGLPGVVAVLTRSDLDGVPAIPIRNFAVPGMEDHLQPVLAGGEVRYVGE
                     PVAVVVAEDRSVAEDAAAAVELEMDLLPVAGEVSLEGVPEDRVMCRWTGRDGDVDAVF
                     EEAAVVVSGSFGTQRHTAVPLETRGITARWGRDGLSLWGPTKFLSFTQRTVADWFGLR
                     PERVHLHRVDVGGMFGVRGELYPEDFLVPWAAAVVGRPVRWVEDRQEHFGAANHGREV
                     RYRFELAAADGRLLAFRARVVVDMGAYVRPAGGRVPLLVIEELPGPYHWRAFEAMCEG
                     IVTNKTPVGTIRGPSALESTFVRERAIDLVAERLGVGSAEVRRASLIDHGGRPYRRSF
                     GEQIHPQVLEPADYRHMFDRFLASTGYAARREDAARRRDRGEPAGVGLACFLAHSALG
                     EEEHASVALGTDGTIELATRATEVGQGIDRLLRRVAAEALEMDPERIRVRSGTTDGTP
                     PGRGTFASRSAVFAGHAVRDACGHLIDQARTKAAKERDAEPESFVPGVGGLRSGEIFV
                     SWEDLAPLDADGVHVARRPAFGFGAHVAEVALDRATGALRVEGLAVGYDCGYALDPHG
                     LRAQLVGATAFGFGDTVLEELVYDRDGQLLSATLMDYLLATAAETPSIEAMVLSTSSD
                     PRAAKGAGEAGVLGVAGAIANAVADAAEARAGGLTVLPLSPERLASELGSAPSPRRGS
                     A"
     gene            22276..>22958
                     /locus_tag="VE646_02820"
     CDS             22276..>22958
                     /locus_tag="VE646_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HZD78961.1"
                     /translation="MNRDVAKRVAEAAEANDLDAVVALSPENVAYLLGFVVPSQPSMR
                     WRHAGVVLTREARSGILAVDMEAATVREREPDADIRVWHEFGHDAVDVLIDLLRDLGL
                     TGSRVGIETEYVPARDMDALRSGLRGVRWEPAGALFGRSRMIKTPREVELMRGLSRLT
                     DRAIWEALGSVREGDTERDLAAAVTGALYRLGAERHDLLIVASGERSRLPNVGPTDRV
                     LRSGDLVRL"
BASE COUNT         3348 a         8154 c         8141 g         3315 t
ORIGIN      
        1 accttggcca tggcgcggcc gtggatgtgc tcggacgccg cgacctcctg cgcggtgaac
       61 gcctgcgtgt gctcctggat ctcgagctcc acgccctgat cccggaggaa cttctcgagc
      121 ttcgtcctgc agtccatgac ggcctcctcg gggatggtcg gtgggctcac gacccgatgc
      181 ccctcgtcgc cggtgcgctg gaccgatgaa cgcggctcgt ccacccatgg ccgccacctc
      241 catgggcagc tctgttcgtg tccactctac ttcgtgcggc acccctcccg cccgaaggga
      301 cgcggggagg ccgcgcggag ttcggatctt ccgcctcagc ccttctgccc gggcatcacg
      361 tcctgctccg agcggtgcca cacggcgctg tcgcgcgcgg tctgcacgtt gaacccgtag
      421 tcgtagacca gcgagcctcc gatcgtcgct cccagggccg tcatggccgc caccacgatg
      481 gtgagcacgg cgatccctgc cgggaccgag ggctcgctgt aggaagccag tcggatcccg
      541 atgtccgcca gcaccaggac cgtgacggtg gccatggtca gaccatgggc gctggcggta
      601 cggcgcgcct gcgtgccctt gggggccgac cggtaccagt ccaggaagcc ggtcagcgcc
      661 gtgcccaacg agaccaccgc cccgccgatc agggcgaagg tggcggcccg gtagaagtcc
      721 cgcgaccact cctgcgaggc ccagatccac gagaccaggt cgaaggctgc ggccaacacg
      781 tacgccccga tggggatgtc ggtgagcggc gggtggaagg gcttgccggc gaacccccga
      841 aggcccatgg aggggcgtcc ccgcaggctg agcgcgggcc ggaacgaaac gcgtctgggc
      901 atggctgatc ctccctccgg gcgggcaggg gcccactcaa tcacaccggc ccggacgctg
      961 caccctcagg acgtgtactg gaacagctcc accaggttgc cggatgggtc catgagcagg
     1021 acctggtcgc caccgacgcc gcgcaccagc tcgttgcgga accgggcgcc caggccgctc
     1081 aggcgctcga tctcggccgg gaggtcgggg acctccagag agatccggtt ccagccgccg
     1141 ggttccgggc tggtgccgtc ggccatgcgc tgacctccgc cgccctgtcc gctgggggag
     1201 ctgagcagca gacgcaggtc gccccgggcc aagatggcaa aggtgggggc ggggtgcatc
     1261 ccgacttcga agccgagcag gtcgcggtag aagccgatcg cctcatccac gtcgttgacg
     1321 atgtaccgaa cgctgaccgg actcatcgcg cccctcccat cctcgctgcg gatcctcacg
     1381 gtccaggcgt accacggatc ggacgatggc gccgggtcag gggaccggtt cgaaggcctc
     1441 ggcgaactgg ctctggtaca ggtcgtggta gaagccccgg cgtgccagga gctcctcgtg
     1501 gtcgccctgc tcgatgatcg ccccgtggtt catcaccagg atcgtgcggg ccccccggat
     1561 cgtggacagg cggtgggcga tcacgaagct cgtcctgccc cgcatcagct cggacatggc
     1621 ctcctggatc agcacctcgg tccgcgtgtc gaccgagctg gtggcctcgt ccaggatcag
     1681 gatctgcgga tcggccagga aagcgcgggc gatcgtgagc agctgcttct cgccctggga
     1741 cacgttcgac gcttcgtcgt cgatcaccgt ctcgtagccg tcgggcaggg tgtgcacgaa
     1801 gtgatccacc cgcgccgcgc cggcggccgc ctcgatctcg ccgtcgctcg ccccctcccg
     1861 tccataggcg atgttctcgc ggatcgtgcc gtggaacagc caggggtcct gcagcaccat
     1921 gccgaacagg cgacggaggc cgctccggct catgccgcgg atcggcacgc cgtcgatgga
     1981 gatccgcccg ctgtcgatct cgtagaagcg aaggagcagg ttcaccagcg tggtcttgcc
     2041 cgcaccggtg ggccccacga tggcgatggt ctctccggcg ggcacctcga ggctcaggcg
     2101 ctcgatcagg ggagcgtccc tcaggtaccg gaacgacaca tcccgcagcc cgatgtgccc
     2161 ctccgtggtg gccaacacct ccggccgcac ggggtcgggc tcctcctcgg cctcgtccaa
     2221 cagctcgaac acgcgctccg cggaggccac cgccgactgg agcatgttca tgatcgaggc
     2281 ggtctgggcg atgggctgcg tgaactgccg cgcgtactgg atgtacgcga cgacgtcgcc
     2341 cagcgacagc gtgccgttcg tcacgcggat cccgccgatc accgcgatgg cgacgtagtt
     2401 caggttcgag atgaacatca tcgagggctg gatgatcccg gagatgaact gggcccggaa
     2461 gctcgcctgg tagaggtcct cgttctgctg gtcgaaggac gcgatcgccc gaggctgctt
     2521 gccgaagacc ttcacgatct catggcctgt gaacatctcc tcgatgtagc cgtccacgtc
     2581 gccggtgcgc tcccactggg ccgcgaactg cttctgggag cgtttggcga tggtgaccgt
     2641 gaccacgaag gacaccggca ccgccaccaa cgagatcagg gccagcaccg ggctgatcgt
     2701 gaccatcagg acgagcacgc ccacgatcgt gagcaccgcg gtcaccacct gggtcaggct
     2761 ctgctggagc gtggtggaga tgttgtcgat gtcgttcgtc acgcgcgaga gcaggtcccc
     2821 tcgcgggtga tcgtcgtagt accggagcgg caagcgggcc agcttgcggt ccacgtcctc
     2881 ccgcagccgg aacatcgtgc gctgtgcgac gccggccatc aggtactgct ggagccaccc
     2941 gaaagcggcg ctcaacaggt acaccccggc cagggtgagc aagatgccct cgatccgggc
     3001 gaagtcgatc cccgcgcccg gcgtgaggtg catgccggac agcatctcgg ccatctgcgt
     3061 ctgaccgcgc gcctgcagcc cggcgatcac ctgctgctgg ctgacgccgg ccggcagctg
     3121 cctggagatc acgccttcga agaggacgtt cgcggcgttg cccaggatct tgggccccac
     3181 gatggtgaag atcacactgg cgatgccgaa cgcgattacc agcagcagct tgttccgctc
     3241 cgggcggagt cgcccggcca gccggagcgt cgagcctcgg aagtccttgg acctggccgc
     3301 cggggtcatc atgcccggat gggcgaatcc ccgtccgccc atcatcgggc cgcgcgctcg
     3361 tcgctcgggg tgctggctca tgcgacctcc tccggcgaca gctgcgagaa gacgatctgg
     3421 cggtaggtct cgcaggtctc gagcagctcc tggtgagtgc cgatgccggc cacccggccc
     3481 tcggccagca ccacgatgcg gtcggcgtgc atgatggtgc ccacccgttg cgccacgatc
     3541 accaccgtgg tgtcgcgggt ctcggcccgg agggcggtgc gcagccgggc atcggtggcg
     3601 aagtccagcg cggagaagct gtcgtcgaac acgtagatct gggggcgctt caccaccgcc
     3661 cgcgccatgg ccaggcgctg gcgctgaccc ccggagacgt tggcccctcc ctgagcgatg
     3721 gggccgtcca gtccttcggg catctcgcgc acgaagtcgg cgccctgagc cacggtgagg
     3781 gcatgccaga cctcttcgtc gctggcgtcc acgtggccgt cgcgcacgtt gctgcccacg
     3841 ctgccggcga agaggaacgc gcgctgggga accaagccga tccgggacca gaggtcctgc
     3901 tgggcgagct cccggacgtc gacgccatcc accagcacgg caccatcagt gacgtcgtac
     3961 aggcgcggga tcaggttcac gagggtggac ttgccgctgc cggtgctgcc cacgatcgcc
     4021 gtggtctctc cggggccggc ggcgaacgag atgcccgaca ggacgggctc gtctgccccc
     4081 gggtagcgga aggtgacgtc ccggaactcc agggtgccga ggtgctcggg gctcgggacc
     4141 ggaaccgcgg ggtcgatcac ggtgggctcg acctccagca cctcctggat ccggtcggcc
     4201 gacgccgcgg cccgcggcac catcacgaac atgatcgtgg ccatcagcac cgcgaacagg
     4261 atctgcatca ggtaggtgag gaaggcggtg aggtcgccga tcggcatccg cccctcgccc
     4321 acctggaagc tgccgaacca catcacggcc acggtggaga ggttcaggat cagcatgatc
     4381 gctggcatca gcagggcgaa cagccgcatc acccgcagcg aggtatcggt gaggtcggcg
     4441 ttcgcgaccc cgtaccgctg ctcctcgaac cgggtgcgat cgaaggcgcg gatcacccgg
     4501 acgccggcga gcttctcgcg catcaccagg ttgatgcggt ccagcttcac ctggagctgc
     4561 cggaacatcg ggaccgcccg ggtcacgatg aagcccacca cggcggccat cagcggcagg
     4621 atcaccacca acagcaggga cagccggggg ttgaggcgga gggccatcac gatgccgccg
     4681 acggccatga tcggcgccag gatcatcacg gtgagcgcga tcatcaccag catctggacc
     4741 tgctggacgt cgttcgtggt gcgggtgatc agcgaggggg tgccgaaccg attgacctcc
     4801 atctgggaga acgactccac ccgccggaag agggcaccgc ggacgtcgcg cccgaaggcc
     4861 atggccgtgc gagcggccca gtacaccgcg atcacggcag cgacgcccat gagcagggtg
     4921 aacccgagca tcaggccgcc gatcgagacg atgtagtgcg tgtcgccctt ggcgatgccg
     4981 ttgtcgatga tgtccgcgtt cagggttggc aggtacaggt tcgcgacggc ctgggcaagc
     5041 agcagcgtga ccacgatgac gagctgccgc cggtaggggc gcaggtgggc gatcagcagc
     5101 cgtcgcacgg ctccggcctc accgggctgg gcggcctcgg cgcccccgcg ggagcgcagc
     5161 cggacggcag catggttgct ggcaccatgc aactatagct gacatacttg gcctgtgggt
     5221 ggttcggctc gtacagaggc cctggccgca gtggagaacg cgttctcccg gttcatggcc
     5281 cgatcctcgc tcccgcgcct gcgtcagcgg gtgaccgagg cggtgggggc cggggtggat
     5341 ttctcggcct tccccacgct ggcgcgggtg gcctcttggg gccccctgcg gaccagcgag
     5401 ctggccgagc ggatgggcct ggacgtgtcc acggtcagcc gacggatcgc cgacctggaa
     5461 caggccggcc tggtggaccg agcgcccgac cccgacgatc gtcgagcgca cctgcttcag
     5521 atcaccgggg cggggacgcg gttggtggag cgcatgcggc aggtccgcag cgggctcctg
     5581 gaagaggccc tcgggggatg gtcgaccgag cagatccagg agctggcgga cgtgctcggc
     5641 cgcttcacct cggccctggc cgagctctcc tgaggacgag ctccgtccgc cggctcccgg
     5701 agggtccgcc cgctaggctc ccgtcgccgc gaaccacacc gtcggggacg gccccacgaa
     5761 cacgaccggg gcgatgcacg ggctcggaag gtccagcgtc gcctcgatcg tggaatcacc
     5821 ggacgtgctc gcggggaacg ggtcggtggc cacgttggtg gtagctgccg tcccgtgatc
     5881 gatggtcagg cagctcacga ccgcctggaa ggcggggatg gggttggtgc cccgcagcgg
     5941 ctccgggacg ggatcgtcgt ccaccaggac caagccgcgg accgcgaccg tgaggtgccc
     6001 gtcgctcctg agcttgccgt gagcgcgatc gagcttccag gcgaaccctc caccggccag
     6061 tccgcggacc gggttgtcgc ctcccacgaa gggcccggtg accggggcca tcgtccggaa
     6121 cttgagcacc gcccgaggat cgtcatccct tgaggtggcc cctgccgccg ccgatatcga
     6181 gccgatcacc aggaccgtcc ccagcacaac gctcagccac cgtctcatgc cgctccctcc
     6241 catccggacc ggcccgcccg gtccacggtg gaactacgga cgagccggca tccgggatct
     6301 ggtcgttccg gaccccgacc acagccggcc gatcagctcg atcgggaacg gcggctgggc
     6361 cagcggacgg accgccagcc ggagcaggat gagcgggttg tcgtctccgg ccgcgcggtt
     6421 caggtgcacc tcgtcgcagc cggtgcagcg gtgcacgatg acccattctc cgtcgcccct
     6481 gaccgcgatc gcgatcggct ccatcagcga tccgcacgcc gccgtccggt cgcccggtgt
     6541 gtcgtcgagg tgccggctcc acaggcagct ggggcagtgg ttgcggtgtg ccgtcccgac
     6601 ggcgcgcgtg gacacgtcca gccggcagtg gccgcagcgg aacgtgctcg gttcgtgccg
     6661 ccggggaggg cggcgctggt cgcgtgacac gggtgacctc cgggatgaga gcgacggggg
     6721 gacgtgcggt cgcgccgggg gtcggtcctc gaaacgatca gcgggagcgg ggggcggaac
     6781 ccgggcgcga cgcgacgacc atggcgatca cggtcatcgg tcacctcctc gggtcctgcc
     6841 gcgtggatcg cggcaaccca tctacgttag ccgatgcacg gacggcgtgc aacgggcggt
     6901 cgaccgcttc ctccgagggc agccgggggg agcgccccgc cgaaagcggt cgctaccatg
     6961 ccacggttcc tgatcaggag aaggggggcc tcgcccatgg tcaagaggac cgcttccatc
     7021 gcggtcggcg cgatcctgct gtgggtggtg gtcgtcccgg cgatcccggc atcggcgatc
     7081 accaccggac gacccgacgc cggagagcat ccctacgtcg gcgagctgat cttctatgac
     7141 gccgactacg tcgatccccg tttcgacgag ccgggcgggt ggttcagctg ctcggccacg
     7201 atgctgtcgc ccgacgtgat cgtgacggcc ggccactgca cctacggggt gggccgcgac
     7261 ggggagtcga ccaccagcgg ttccgggaac gggtccggcg gcaacgacat ctggttcgac
     7321 aacaacgaga acgccgactt cgagggcttc ccgccgaact cggattacgg tccggaccag
     7381 aacccccagc gttaccagga ccgcgcccgg ttcctgaacc ggagtccgta ctggcaccgc
     7441 ggcacggcct acccccaccc ccggttcgcc gatgcgccct tctacgtcta cgacgccggc
     7501 gtggtggtcc tggaccagcg gttgcgtgtc ggcgactacg ggaggatccc caccctcgac
     7561 tacctggacc ggtaccaggg cgctccacac cacctgctgg aggtcgtggg ctacgggctg
     7621 acggcgagcg gtcccttcac cgaggagggc ggcgacgtcc ggatgaaggg cgacgtcaag
     7681 atcgacaccc tgaacagcgc ccccaaggac acgttcatcc tgctgtccaa caacaagggc
     7741 aagccccatc gaggcggcac gtgcttcggc gactcgggtg ggccgacctt cgacggcacg
     7801 gactcgaacc tggtggtcgc ggtgacgtcg ttcgggttca gttcgacctg ctccggggtc
     7861 ggcggcgcct accggatcga ccaaccggac gatcagcgct tcttggcacg gttcggtatc
     7921 aggccttaat cagaggctga cacgcgaccc ggacgcggac ggatgagatc cccgtccgcg
     7981 tccggggtcg agtggctcgg gcgatcggac ctaccagtac cgggcggtcg ccatcgcgat
     8041 gaccgccagg atcagcagca ccaggtccag cttgccgtga cgctccagcg cgctcgagat
     8101 ccggttcagg gcctgctcct ggttcgtggt gggcggggcc cccgacgcgg cgatctcgcc
     8161 gctcaggtcg accaggcgct ccacgttggc gcccaccccg atggacccga cgaaggcgga
     8221 ggtggttgcc agcacggcgc cgatcgtgat gaccaggccg aacctcgagc tgacccagtg
     8281 acccacggag ccgtacaggc ccatgtcatg cagccacatg gtccagccgg cgaggacgtt
     8341 gacgatgccg agacccgtga tgacgcgggc cacctttcgc ttcttcaccg ctgcggtcag
     8401 cagggggacg gcgctcggtc ccacctcggc cgcggagggc ccgatgaatc ccacgaacag
     8461 gaaagccgag cccacccaca ggaccccggc agcgatatgg aagaaccgga acacgagcat
     8521 gaacgtggac gtggcgagca aacccttcct ccttccgatc gtcggacggc caaccctatc
     8581 ccgttgggcc cgggacggtg cgtcggcggg cgagccgtcc tcacgcgtga gatccctgcc
     8641 ggcgagcacg cccgaggtga ggctctccgg cgtcgcccgg tccggtactg tgtgcggccg
     8701 gaggtgggga ccgtgacgga tcatcgaacc atcgtgttct tcccggagcc gggggcgtgg
     8761 gggcccacga acaactgcgt ggcgatcgcg gaggtgctgc gcgagcgggg tcaccgggtg
     8821 gtcttcgtgg cggacgccag cttcgaaggc gtgctggaag gtaagggctt cgaggagcgc
     8881 gtgatgcgga tggcagcgcc cgacgagaac gcccacccca ccgcggaccc ctgggccgag
     8941 ttcatccggg tgaccgcccc cgagttccgc aagccgacga tcgagcagca ggccaccgtg
     9001 acccgcccca tctgggaggc gctggtggcc ggcgcccgct attcgcacca gcgcgtgatg
     9061 gagatctggg ctgacgtccg cccggatgtc gtctgtaccg acaacgtcac cggtttcccg
     9121 gccgtcgagc tcgccggctg cccgtgggcg cggttcgtct cggccaaccc gctggagatg
     9181 cgcgatccgg atctgccgcc gcctctctcc ggactgcccg tggccgaccg gagcgggtgg
     9241 caggcgttcg tggacgagta ccaccgggtc cacgaggacc tgctggccga gcacaacggc
     9301 ttccgcagct cgttcggtgt ggcgccctgc ccgtcggacg agttcaacac ccactcaccg
     9361 tggctgaacc tgtacctgtt cccggaggtc gccgactatc gtcgcgcccg tccgctggac
     9421 cggacctggc accggctgca gtcgacggtc cgcaggggcg aagccccctt cgacgtgaac
     9481 gagcatgtgc ccggtgatgg gaaggtggtg tacctgtccc tcggctcgct gggctgcatg
     9541 gacgtcgggc tgatgcagcg gctgatcgac gccctcggga ccaccgagca ccgggtgatc
     9601 gtctcgctgg ggcccctcgg ggagcagatg acgctgggac ccaggatgta cggcgagagc
     9661 ttcctgcctc agccctcgat cctgccgcag tgcgacctgc tgatcacgca cggcgggaac
     9721 aacaccacgt gcgaggggtt ccacttcggt ctgccgatga tcggcctgcc gctgttctgg
     9781 gaccagtacg ataacgcgca gcgcctgcag gagacggggt tcgggctgcg actgccgacc
     9841 tacgactggg aggagggaga gctggtcggc gcggtcgagc gtctgctggc cgaccaggac
     9901 ctgacggagc ggatgcgttc gaacgccgag gcgatccgct ccgacccggg cgcagtgcgg
     9961 gctgcggacc tgctggaacg cttggcggtc accggcgagc cggtggtcga ctgagggcgg
    10021 gccgcctggt cacccggaga ccggccaccg atcgcgctcg gcgacccaga tcatggagcg
    10081 gccggtctcg agcggtgcct tcacctggtc gggctcgatc cggtccaggc ggtagcccag
    10141 gcgcctcggg atcgcggcgc tccggacgtt cgcctcgtcg cagtggatct cgacctggtc
    10201 gacgccgttc agcccgaggg ccgcctccgt cagcaggcgc gccgactcgg tggcgatgcc
    10261 ccgcccggtg tgacgcgcat ccacccagta gccgatctcg agcgctccct cgccgatccg
    10321 gggcatcagc ccgcatccgc ccacgtaggt cccgtcctcg gccagcgtga tcgcgtagtt
    10381 ggcggccttc gtcccgccga aggcctcgat ggacggggtc aggaactcga ggaccgagcc
    10441 gtccgtcggc ggcgtcgccg cccagggcat ccatcctctc agatgctcgt gactcgcggt
    10501 gatcgacgcc ttcatctcgt cgaggtcgga cggccggtac gtccgcagcc ggacgagctc
    10561 gccgcggagc tcctggggtg ggtggaagcg catcggcgca tcctagcccg gacgggccga
    10621 cggccggggc cgccaggcag ccctcgccac ggatggctct ccggtcagcc cgtgggcgat
    10681 gtgaggcagc cgatatcgac ctcgacctcg cgcaggtaga gaccgcaacg gcacgccgcc
    10741 aggatctccc gcgcgcgttc cagggtccat cgggggtcga cgccgagccg acgctccgct
    10801 cgacccagct cgatcaggca gcggcccagg tggatccgca tcccgatcga cgccagcgcc
    10861 gtcgctgcct cgcgcagctc ggacacctgc tccgcggggt ccgcggcgag cagtccttgg
    10921 gtgatccgga cgaaggcccg catgtgcggt gggaaacggt cggggtcacc gagcgaggcg
    10981 gcctcctcgt gagcctgctc cgctcgatcg aaggccagca ggatccgcac gcgctcgcac
    11041 aacgggagtg ggaggagcac gaggctcgca tctcgtacca ccagtcgaca gctctcggcc
    11101 gacctttcca gggccgcgtc ggcatcacca cgcgccgccg ccgtcagccc accgaacatc
    11161 ttctgccacc agaaggtctg gggctccggg ctcgcctcgc tgagccggac ggactcctga
    11221 tacagctcct ccgcttcgtc gacctcacca cgcaggagtc gggccgatcc caggctcggc
    11281 atgcgcatcg cgatggttag ttcctcgccg atcgcacgcg cgagctccag cgactccgtc
    11341 aggtacccct ccgacctcgg gaggtccccc gagtagtcac gcgccaggtc gccgaggttg
    11401 ccgagaagga acgtctgcca gctgcgatcg ccggccttcc gcgctgtctc gagcgcgtcc
    11461 gcatagatgc cctcggccct gtggaagtcg ccggcgaagt cggccagtat cgatgcgagg
    11521 ttgttctccg accggagcac aaggtcgaag tcgcccgtcc gcttggcgag accgacaccc
    11581 cgctccagga gcccgatccc ctcgtcgcga tcggtggccg ccaccacaga cccgagggtg
    11641 atggtcgcat ccgccaccac ctcgagcgcg ccgacccgct cggcgacctc gatggaacgg
    11701 tgcaggatgg cttccgcctc gtcgttctgc ccacatcgcc atcggatgcg ccccaggtag
    11761 tccagcgccc gggccagcgc ttccccttcg ccgagcggct ccaggcgccg gacggcctcc
    11821 tccgcgagct cgatcgcctc gtccagcaca ccccgcacgt acgcgatcag gctgagctcg
    11881 cactcggcct tgcccgcgct cgcgtcatca ccgagggcgt cggcgagctc gcggacccgg
    11941 gtgaacgcct cccgggtcac gacccatgtc tcgaccccgt ggagcgaggt ggcgtaggcg
    12001 gtgcgcagcc ggaggagctc gaagggctcg agtgcggcca ggtcgctcag ctccagcgcg
    12061 tctcggtacc agcccagcgc ggatgagcgt tgccagagcc ggagggctcg gtccccggcc
    12121 gatcggagcc acgcgagcgc ctccatggcg agcccggggt gatcgtcgac cgggcgaccg
    12181 agctcggcca ggtaccggag tgcctggcgg tagtgcgacg cgatgagctc ggcgagctcc
    12241 tctccccgat cgccaacacg cttggcggcc catcgggcgg tggccagatg ctgctccacc
    12301 ctcgccgcct tggggaggga ctcgtaggcg ccgtcgcgga tcagcacgtg tcggaacccg
    12361 agctcgagct ccccggagaa ggtggaaggc tcccggggga cgacgagctc cttcacccgg
    12421 agccgaccga gcacctcccg cgcctgatcc tggttccacc cgctcagtcg ctcgagggcg
    12481 ccggaccaga agatccgccc cacgacggat gcgtgctgga gcagcttctt ctcctcagcg
    12541 gggagcgtgt ccagccgagc ggcgatgagc gcgccgatcg agcgcggcag cgcgacctcc
    12601 tccaccggca gggcgacctc ccagcgggag gcctcggtcg cccgcagcac ccctcggtcg
    12661 atgagcgagc gcacgatctc ctcgatgaac agcgggttgc cctcgctccg ctccacgatg
    12721 gcgtcctcga tcccggccga gagctggccg ccgagcagat cctgcagcat cgcgcgatcc
    12781 tcgtcggacc tcaggggctc gaggaagatc gacgtgacgt tgcgcttgcc cccaccccag
    12841 gtcgggcgga tctcgagcaa ctccggacga gaggtgccca ggatcagcac cggtccctcg
    12901 gcccagtccg ccaggtgctc gaggaagtcg agcagaccgg ggtcggccca gtgcagatcc
    12961 tcgatcgcga gcaccagcgg gtggcgagcg gccatcgcgt cgaggaaccg gcgccaggcg
    13021 tcgaacagtt cctccctgcg gagggcgacc tcggtcccgg tacctacgag agcggccaga
    13081 tgcggcgcga gctcggtccc gccgaggagc tcgaccgcgg cggcttcgac cttggcctgg
    13141 accacctcgg tcgggtcatc ctcgaggatc tcgcactggg acttcagcgc atcggccagg
    13201 gccgaatagg aaaccgcgcc gtagggaagg caccggccgg agcgccagta gtagacctcg
    13261 ggcgccgatc ccaccacttc gaggagctcc gcgaccaagc gcgacttgcc gagcccagcc
    13321 gagccgacga tcgtgacgag cgctggtcgc ccctcggaga tgacccgctg cagggtctgg
    13381 ttgagcagcg cgagctccgc gtcccggccg accatccgcg cccggatccc cagggccgca
    13441 cgcacgccgc ggatccgggc gcaaacgagg agcgcgggcc aggtcggcac gggctcggcc
    13501 ttgcccttga gtgcgagcac cgtgggctcc ccgtactcga cgatcgcctt ggtcgccgcg
    13561 tggacgaccg ggcccacgag gatctgaccg ggctcggcgg cggcctgcag gcgtgccgcg
    13621 gtgttcacgg catcgccggt caacatccga tcgcgtggcc cctccacgcg ctccaggtcg
    13681 gccaggacat cgccggcctc gacgccgatc cgcatcgcga gctgcggtgc gccctccgaa
    13741 gcgaaggtcc ggttcgcctc ggccagcagg tcctgcagct ccagggcggc gcgcaccgct
    13801 cgctcggggt catcctcgtg cgaccgaggg acgccgaaca ccgcgaggac ggcatcgccc
    13861 atgaacttct cgaccagccc gccgaagcgc tcgatcgtct ccgcgagccg gtcgaacgct
    13921 caagcgatca ccgagcggac gacctccgga tcggtgcgct cggccagcgc ggtggatccc
    13981 accagatcgg cgaagagggc ggtcgcgaac ttgcgctcca tgcgagggcc agcctgggag
    14041 ccgagcgcct tgccgcagga cagacagaac ctcgcccgct ccgggttgtc ctcaccgcag
    14101 ctcgggcaga ccgccaccag gcaagtctac gtccggcgtc tgtcggccgg tcggccggcc
    14161 catcggccgc tggatcagcg ggccgctggg aggggcaggc tggtcggcgt gggctgggat
    14221 gccggagtgg gagcggcccc ggtccggccg gcgttgccgg atccttcggc gctgcggccc
    14281 tggctctcct ggtcggcgat ggaggttccc acctcggccg cgccgccgct ggccgcgttg
    14341 gccgttgcgg tcccgcccgg gttcggcgtg gtgaccggcg gggcctccga tggccgggcc
    14401 tgagaggtgg ctccgtgctg acccgcctgg ctcatgccgc cgctggcggc atcggagatc
    14461 tgtgctccct tggccacgcc cgtagcgtcg gtggaggtgg ccatctggga gatgtcggtt
    14521 ccggtgctgg cggggtgctc gatcgaggga atcgtgatgc cgacctggga cagcgcatcg
    14581 gacaccacgt tctgggccgg gtcggggagg accccggctg cggccaggcc ggtggtaccg
    14641 acgagggtgc cgatcacgag gataccggtg agcttggcgc ggaagtgctt gggacgcacg
    14701 gtcgacctcc ggggggtagg ggtgacgggc acgggtccga gagcgctgcg gatcccgctg
    14761 gctgccgaca ccgcccggtc ctggtggagc agttcccgtg cggaggccgg cgcgccgacg
    14821 tcacgcatca gcccagccac ctcctggtag ccgggagggg cgtcgtcggg atccacgtga
    14881 ccgtccagca gccgatcggc cgtgtcgcgg tcgagccacg gaacgcgcat ctcatcttcc
    14941 caatcgatcg ggccggtcat cgcgttacac cctgctccgc catcgtcctg gccagccgac
    15001 ggagggcgcg atgctggagg acccgaaccg ttcccggccg cttgcccatg atcctcgcga
    15061 cctggcggac ctcgagtccg gccagggcgc ggagcaacag cacctcggct tgatcggagg
    15121 gcagcgaggc gatccgggac agcgcctcct cggtggccag ggcggccagg gcttcatctt
    15181 ccacgttccc gatccgggcg tgcgtcgcca gccgctcggg gctcaccggt tccgtgcctc
    15241 ggcgtgctcg cctacggcgg agatcgatcc accggcgccg ggcgatcgtg aacatccagg
    15301 ctcggaaggc tcgctcatcc ccctcgaacc ggtccaggcc atcgatcgca tccagccaga
    15361 cctcggaggc cagatcctca gcctccgctc catcgagcgc ccgtaggtag cgcagcacgc
    15421 gagggtgcac gtctcggtac agatcggcga gcgcccatcc cgcacccgac cgggccgagc
    15481 gcaaggtctc ctcgaaccgt tcccccaggc tcatggatgt ggttcagggt accgagccct
    15541 ctccggtacc gcatcgaccg cggggaggtc cgtcggtggc gtcaatcctg ggcgagcgcg
    15601 gccgcgacga tccgggagac gcccgcccat tgggacgcgg cgaccgtcct cggcgccgag
    15661 gctcccaatc ctctggccca gacgtggtcg agcccgagct ccgtggcgac ctccccgtcg
    15721 acgcctccgg gcggtgccga catgtccacg accagcgcgc cccgagggag acggccaagc
    15781 gcccctcggg ataggacccg cgccgggacc gtgttgaaca cgatcgccat cccgcggaac
    15841 aggtcggcga tgcccgcgag gtccgtggcc tgcgcgccca tggcgatcgc ctcggctcgg
    15901 gcctccggcc ggcgggcgac cacgtgcacc cgcgcgccga gcgcgagaag cgtgcgcgtc
    15961 aggatccggg ccgtacgtcc gaagccgatc acggcgatcg gcgagccgtg gatcgtgacc
    16021 gggctccgct ggatcgcgac ggcgatcgcg ccctcggcga tggccggggt ccgcagcagc
    16081 atgagctcgg tgtcgtgctc gtactcgtgg acgcggaccc cgcagtccgc ggccgcctgc
    16141 cggaggccgg ggtctgcctt gccgatcacc acgtgggccg agagcgccat cctgcgcagg
    16201 acctcggccg tgaggtagat cggttccgcg gcggaggggg cgaagatccg accgtccggt
    16261 ccggggagcg ggatcggtcc caccgccacc cgagcccccg agagcgcctc ggcgagcgag
    16321 tccaccaacc cccccgaggg gcgcccgaca tcgacctcgg gggtaccgaa ccgccgtacc
    16381 gctgcaccct ccgccacggc cagggcggcc atctccacct cacgggcgtc cccgcccagg
    16441 aacgccaccg agagccccgc ccacaccgtc acaggtgttc caggtggtgg gtgaacgcct
    16501 ccacgtccat gccggcgatg ccgagcacct ccgcggtgag gccctcccgc cggtaggggc
    16561 gtgcggtgat ggtctcggcc agcacgatca gggcctcggc caccggggtg gggatcccgg
    16621 cgatccgcgc cagcgccacc agggggacga gcccgaaccc cacatcctcg tgcaggtacc
    16681 gatgatccac gctcgtgggg gcttggatcc ccgcgttcgc ggcgcctccc cggatcgccc
    16741 ccctgaggtc ccccgcccgc gcgcggtcca ggtcggccga cccgtacgcc gccatcgtgt
    16801 cggtcagcgc aggcaggtcc aggccgaagg cccgaccgac ggctcgccgc tccatgtcca
    16861 aggcgagcat ggtccgctcc accgacggcg tgacgccctg cgtgtagaag ccgaatccgc
    16921 cgtcggtgcc ctcgatccag gcggcaccga gcagcatccc cggtggatgc agcacgaggt
    16981 tcacgtcgtg gagccaggtc tcgacgacgc cggcggcccg ggtgcccggg aacaggtctg
    17041 cggtgagggc ccggacctcg ggccggatcc ggccggacac cgccatgggg acctcgcgtg
    17101 tgcggaggga gacctggacc gaggccgggg cgctggagcg acagatgtag ctgagggtgc
    17161 cgagctccgc gcagggcggg accggcctcc cggcgatctc gaacatccgg cgcaggtgga
    17221 gggacccgca catgtgcccg gggtcgagca cgatcgggag ctcgcccagc atcgtggagg
    17281 ccagggccgc gaagacctca ccgtgcgccg gggccgggag cacgatcacc accacgtccg
    17341 cccccgcgat cgccctcccc agatcggtgg tcgcggtgtc gatgcggacc aggccctcgc
    17401 cgatcgctcc ggaggagcgg atcccgcctg cctcggtgac ccgctccacc ggctccgggc
    17461 cccggttcca cagccgaacg tggaacccac gccgggccag gtcggcggcc gcggccagcc
    17521 cgccgtggcc ggcccccagc acggccaccg ccacggtccc ggtggcggtt ccgctcatcc
    17581 gctcaggccc cccggtccac ggggcgatca cctcgcgtag gcacggacct tctcctcgtc
    17641 cacctcgatc cccaggcccg ggccctcggg cacgagcagg gcgccccgct cgaaccggag
    17701 gggctcggcg atcacgtcgt cggtgtagta gttgccggcg atccccgggc cggcctcctt
    17761 cgggctcgag accggacaga ccgaaggcag gacggcgatg ggcgtggccg cgccgagctg
    17821 caggttggcg gcgttcccga tccccatctc gatcgagccg ccgatgtccg aggtcatgcc
    17881 gaaggcctcg gccacgaccg cctgctcgcg agcccgatgc aggccgcccg gcttcgcgac
    17941 gtacagggag atcacctccg cggcgtgcag ctcggcgagc tcgatcacgt cctgcggggt
    18001 ccaggccgat tcatccgcca tcaccggcac gtcgatccgc cgggccacct ctgccagcgc
    18061 gcgggctccc cgcatcggct gctcgcacag gaagatgccg gcctccgcca cctgccggcg
    18121 ggtgacgccc acggcctcgg ccacgctcgc gtagccctcg ttggcgtcca cgcggatccg
    18181 aacgtcgtcg cccagatgct cgcgcaggcg tcgcacgagg cggacgtccc gctcgggatc
    18241 cacgccggtc ttgaccttga tcgtcccgat gccctcggcc accgcgcctt cggcctcgtc
    18301 cagggcggga tcgtcgtcca tgatccccag ggagtggcag acctcgaccc gatctcggac
    18361 ccgcccgccg agcagcgtcg agaccgggac cccgagggcc cggccggtca ggtcgaagca
    18421 ggcgatgtcc agggcggcct tcgcgtaggg gttccccttc accaccgagt ccatcgcccc
    18481 gtgcaggacc gccggctcga gcggggaccg tccctcgatc gccggccaca ggtggtctcg
    18541 gatcatcagc tctgctgtgt tcggggcctc tcccgagtag cgcatgtgcg gcccgcccca
    18601 ggtggggatg gccggcgcct cgccccagcc gacgtggccc tcgtcgtcac ccaggcggac
    18661 cagcagatgg ctgccgatcg gcgtgctcat cttgccggcc cagttgtggg cccgacgctt
    18721 cggcaggtcg acgatgaacg tctggatcga agtgagcttc actggtgcag ctcgatcttg
    18781 ttcccgtccg ggtccaggaa gtacagggtc gtcccgtacg ggccgggacc gggacccttg
    18841 acgatccgcc ccccggcgcc acggacgcgc tcggccacgg cctcgacgtc gctgacccgg
    18901 aacgcgatgt gctccacggg accgggcggc tcgggcccgc cctgggtgag ccccaggccc
    18961 tgctcggtca cggtcaggtc tcggccgtcg gccagggtcg cccgcttgcg gaccacgaag
    19021 cccagcccgt tcacgtagaa ggcctcggcc ctggccaggt ccgagacctg cagcagcagg
    19081 tgatgcaggc cgaagacccg agccgggcgg ggagggcccg gctcgcggtc cacgcccagg
    19141 acgccggcac gaccccagtt ctggggcgtg ctctcctgga gcaccacgtg cagcccggag
    19201 ggatcggcgt cggcgtggtc caccatcgcc ttggtgatcg cggcgaccag ggcccgcttc
    19261 tgatcgagcg tccgtccttc ccacagctgg accgtgacga ccggcatcag ccggtgccct
    19321 tggcatcgag catctcgatc tccttccggg tgcgttcgat gtgccggcgc atggctcgcg
    19381 ccgcccccac cggatcacca gagttcacgg ccttcgcgat gcggcggtgg tcctccaggg
    19441 agcgctccgg ccggccgggc atcgcgaaca tcgcgaagcg cgaggtctcc aggaccccgt
    19501 tcagctcgtg gatcagtccg gccaagatcg cgttgccgct gcggtcggcg atcaggccgt
    19561 ggaacgccac gtcggccttc acccgggcct cgaggtcctc gtcatcggcc accgcggtta
    19621 tgggctcggc ctccgcacgc gagcgggagg ccgccgaggc cgccgcggcc gcctccagca
    19681 cctcgcggac gtccaggaga tcgagcacct cgtcctgatg cgaccggagc cacccgcgca
    19741 gcagcgtccc gctcggctcg gtggcgtcct ccagcacgat cgcgccccgg ccctgacgca
    19801 cctccaccgc gcctatgagc tcgaggatcc gcagcccctc gcgaatgctc ggcctggaca
    19861 ccccgaacgc ctccgccaag cgccgctcgg agggcagccg atcgcccggt gcgaactcac
    19921 cggcgcggac ggcggcgagc aggccgtcca ccacgccgcg ggagagccgc tggttcctca
    19981 gggcctgcac catcgccggt gtacttgtaa ggtggtcaga ccacttccgt caagtcgggt
    20041 acgatacggc cccatggcgg gctccccgga ggacgtggcc gacgcgaaac gtctgctgac
    20101 cgggcgaggc aggttcgtgg cggacgtggc ggtgcccgga gccgtctcga tgacggtggt
    20161 ccgcagcccg atcgcccacg gacgtctccg agccacctcg gtggatcgag cccgggggct
    20221 tcccggcgtg gtggcggtgc tcacacgctc cgacctggac ggggtgcccg ccatccccat
    20281 ccgcaacttc gccgtgcccg gcatggagga ccacctgcaa cccgtgctgg ccgggggcga
    20341 ggtccgctac gtgggcgagc cggtcgccgt ggtcgtcgcc gaggaccggt cggtggccga
    20401 ggacgccgct gcggccgtcg agctggagat ggacctgctg ccggtggccg gcgaggtgtc
    20461 actcgagggt gtccccgagg atcgggtgat gtgccgatgg accgggcgcg acggcgacgt
    20521 ggacgcggtc ttcgaggagg cggcggtcgt ggtgagcgga tccttcggca cgcagcggca
    20581 caccgcggtt cccctcgaga cgcgggggat cacagcccga tggggccgag acggcctgtc
    20641 gctgtggggc ccgacgaagt tcctctcgtt cacgcagcgg accgtcgcgg actggttcgg
    20701 gctccgtccg gagcgagtgc atcttcaccg ggtggacgtg ggcggcatgt tcggcgtccg
    20761 cggggagctg tacccggaag acttcctcgt cccgtgggcg gccgccgtgg tggggcggcc
    20821 ggtccgatgg gtggaggacc gacaggagca tttcggggcc gcgaaccacg ggcgggaggt
    20881 gcggtaccgg ttcgagctgg ccgccgccga cggccggctg ctcgccttcc gcgcccgggt
    20941 cgtggtggac atgggagcgt acgtgcgccc ggcgggcggg cgggtccctc tcctggtgat
    21001 cgaggagctg cccggcccgt accactggcg ggccttcgaa gcgatgtgcg aggggatcgt
    21061 gacgaacaag accccggtgg gcacgatccg gggcccctcc gccctggagt cgacgttcgt
    21121 gcgggagcgg gccatcgatc tggtggccga gcggttgggg gtcggctcgg ccgaggtccg
    21181 ccgggccagc ctgatcgacc acggcgggcg gccctacagg cgctccttcg gcgagcagat
    21241 ccacccccag gtgctggagc ccgccgacta ccgacacatg ttcgaccggt tcctggcgtc
    21301 caccggctac gcggcacgcc gggaggacgc cgcccgacgc cgcgatcgcg gcgagccggc
    21361 cggggtgggg ctggcgtgct tcctggcgca ttccgccttg ggggaggagg agcacgcgag
    21421 cgtggcgctg gggacggatg ggacgatcga gctggccacg cgggccaccg aggtcggaca
    21481 ggggatcgat cggctcctgc gacgggtggc cgcggaggcg ctcgagatgg atccggagcg
    21541 gatccgggtc cgctccggca ccacggacgg cacgccgccc ggcaggggga cgttcgccag
    21601 ccgatcggcg gtcttcgccg gccacgcggt gcgcgacgcc tgtgggcacc tgatcgatca
    21661 ggccaggacg aaggcggcca aggagcgcga cgccgagccc gagtcgttcg tgccgggggt
    21721 cggcggtctg cggagcgggg agatcttcgt ctcctgggag gatctggcac cgctggacgc
    21781 ggacggcgtg cacgtggctc ggcgccccgc gttcgggttc ggtgcccacg tggcggaggt
    21841 cgcgctcgat cgggcgacgg gagcgcttcg ggtggagggc ctggcggtgg gctacgactg
    21901 cgggtacgca ctcgatcccc atgggctccg ggcgcagctc gtgggggcga ccgccttcgg
    21961 cttcggcgat acggtgttgg aggagctggt ctacgaccgc gatggccagc tgctgtccgc
    22021 cacgctgatg gactacctgc tggccacggc cgccgagacg cccagcatcg aggcgatggt
    22081 cctgagcacc tcctcggatc cgcgggccgc caagggggcc ggagaagccg gggtgctggg
    22141 ggtggccggc gccatcgcga acgcggtggc ggacgcggcc gaggctcgcg ccggcggcct
    22201 caccgtcctg ccgctgtccc ccgaacgctt ggcgagcgag ctcggatcgg ccccgtcccc
    22261 ccgacgagga tccgcgtgaa ccgggacgtc gcgaagcggg tggccgaagc ggccgaggcg
    22321 aacgatctgg atgcggtcgt ggcgctgagc ccggagaacg tcgcctacct cctggggttc
    22381 gtggtgccct cgcagccctc gatgcgatgg cggcatgccg gggtggtcct cactcgggag
    22441 gcacgctcgg gcatcctggc cgtggacatg gaggccgcca ccgtccgcga gcgcgagccc
    22501 gacgccgaca tccgcgtgtg gcacgagttc gggcacgacg cggtcgatgt cctgatcgac
    22561 ctcctccgcg acctgggact gaccggttcg cgtgttggca tcgagaccga gtacgtgccg
    22621 gcacgggaca tggacgcgct ccgctcgggg ctccgggggg tccggtggga gccggccggg
    22681 gccctgttcg gtcgctcgcg gatgatcaag acgccgcgcg aggtggagct catgcggggg
    22741 ctgagtcgcc tgaccgatcg ggccatctgg gaggcgctgg gctcggtccg cgagggcgat
    22801 accgagcgcg acctggccgc cgcggtgacc ggtgcactgt accgtctggg cgccgagcgc
    22861 cacgacctgc tgatcgtggc cagcggcgag cgcagccggc tgccgaacgt gggtccgacg
    22921 gaccgggtcc tgcgcagcgg cgacctggtg cggctgga
//
LOCUS       DASQQQ010000064         8399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_253547, whole genome shotgun sequence.
ACCESSION   DASQQQ010000064 DASQQQ010000000
VERSION     DASQQQ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8399
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253547"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..588)
                     /locus_tag="VE646_02825"
     CDS             complement(97..588)
                     /locus_tag="VE646_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78962.1"
                     /translation="MNAPRLRRGLFGYSARSVRLSLAARERELSESSGRAAAAEQRAD
                     ALTQRTQAAEARCAELDAELEVAARSAQEARATLAARDGLLDELRVELATSRRLFLRQ
                     VAHGRSLEATVEDLAAKLWQARADSAEGATPGEGSEPLVVLPDLEAQAERSNGHPPAA
                     RPV"
     gene            complement(681..1673)
                     /locus_tag="VE646_02830"
     CDS             complement(681..1673)
                     /locus_tag="VE646_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78963.1"
                     /translation="MGYSRRSVHELLSNRDQIARRASRQARDALDHAERLHAELEDAR
                     RHVAELTERAEAAEAQIASLQAKLRDATRDERRVLDVEARAARLDLALRTARNEVGDY
                     TGRLQAAELRVSELEARMRFLGPERDALRDRVVQLSGELDRVRADRDRLRTEIEALGG
                     QPGGADVTVSLQEADPLTEEVAGALRAAEMAVTKIVESARLKGEEELEELERVQQQVR
                     METDQLMAWRRSAEPTLGDLGGWLRETRRRTADLAEQIREVLEPAASALDELNRRLRD
                     IGDVPGGSPIPSGDQSAHAQAGAPRPIIVPEAQRSDQRAGTDLAAGGVIREAAR"
     gene            complement(1751..2896)
                     /locus_tag="VE646_02835"
     CDS             complement(1751..2896)
                     /locus_tag="VE646_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78964.1"
                     /translation="MEEREYPAEEPELKRRFMGYSPEDVQKLLADREQMLADREQMLA
                     VASESAKIAEARIADLERELETVRANMPRPEYVEDRERALAEVRTQLEQAQDDLTRSR
                     GELEQARSGLADEERRRQAAEHRADQYVAELDELRQDLLTARRDLVSESERASSTESR
                     IEELTSELEAAQARMDITPSISDPSTPDEVTDVLRAAEEVVARVMEGGRARVQQELEE
                     VHRELQEAERARDQVRADIDRLAQWRDRLAPQVEVIRSAIEDAQLHAMRFGERVQEAV
                     HPITAAMQVLGEQLEGLNEAGETGPQGSPEPGASGGPEPDEGDAPELGAPEAGAPEAG
                     AFGGPEGGEGGGLDLRGDDGTATTEASTDGPPLGAGGSDPLREVPKS"
     gene            3199..4359
                     /locus_tag="VE646_02840"
     CDS             3199..4359
                     /locus_tag="VE646_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HZD78965.1"
                     /translation="MEIEIGAGKAARRAYGFDEVAIVPSRRTRDPDDVDISWEIDAYR
                     FDLPMMAAAMDAAVSPATAIEVGRLGGLAVMNLEGLQTRFEDPDPAFEEIAQLPDEKA
                     TRRMQELYREPVKDELITRRIREIKEAGVIAAASLTPQRVGRYAKLVLDAELDILVIQ
                     GTVVSAEHVSSRTEPLNLKEFIAGFDLPVIVGGCASYQTALHLMRTGAVGVLVGVGPG
                     NACTTRGVIGVGVPQATAIADASAARSEHLRETGRYCHVIADGGMRTGGDIAKAIACG
                     ADAVMIGSPLTRAYEAPGRGYHWGMATFHPTLPRGARVKTQQVASLEEILVGPAHEND
                     GTFNLFGALATSMATTGYATIKEFQKAELVVAPSIKTEGKTYQQAQRVGMGH"
     gene            4427..4660
                     /locus_tag="VE646_02845"
     CDS             4427..4660
                     /locus_tag="VE646_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HZD78966.1"
                     /translation="MKTTLNLDDDLMRQAKKRAVEEGTTLTHVVEEALRAALATRGRS
                     PRRTVRWVVVDGGPPRVDVADRDALYDVMERPD"
     gene            4576..5085
                     /locus_tag="VE646_02850"
     CDS             4576..5085
                     /locus_tag="VE646_02850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HZD78967.1"
                     /translation="MGGGRRRPPPRRCGGPRRALRRDGAAGLIAIDTNILVYAHRRQA
                     PQHQRALEALRQLVVGPAAWGVPVFVAGEFLRVVTHPRLFDRPSKLEDALAALDGLLS
                     SGTARLLLPGERYWSILRETALDGRASGNLLFDAQIAAVCIEHGVTTIMTEDRDFRRF
                     SRMQVLPLL"
     gene            5104..6639
                     /gene="guaA"
                     /locus_tag="VE646_02855"
     CDS             5104..6639
                     /gene="guaA"
                     /locus_tag="VE646_02855"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZD78968.1"
                     /translation="MADARPVLVVDLGAQYAQLIARRVRECHVYSEVVPHDVSAQDLR
                     ARGPAGIILSGGPASVYEAGAPDVDPELFHLGVPVLGICYGHQLMSRALGGEVAATGI
                     REYGRTELAVDEPGVLLEDLPPRDTVWMSHGDAVSAAPQGFRVTAHTTSTPVAAMEDR
                     QRGLFGVQFHPEVAHTPKGMDVMKRFLYDGCGLLPDWTPANVIERSVARIRHQVGDRK
                     VICALSGGVDSAVAALLVYKAVGEQLTCVFVDTGLLREGEPEQVEEAFGRHFRVPLVH
                     VKAAERFLDRLAGVTDPEDKRKRIGEEFIRVFEEAAAPIGAGLLVQGTLYPDVIESGS
                     RTAATIKSHHNVGGLPEDLSFELVEPLRDLFKDEVRRVGSELGLPEEIVWRQPFPGPG
                     LAVRIVGEVTAERLEMVRAGDAIVREEIRRAGLERPIWQAFAVLLGEVRSVGVMGDGR
                     TYQHPVVVRAVTSEDAMTADWARLPAEVLDRIATRVIGEVRGVNRVAYDVTSKPPGTI
                     EWE"
     gene            complement(6655..7203)
                     /locus_tag="VE646_02860"
     CDS             complement(6655..7203)
                     /locus_tag="VE646_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZD78969.1"
                     /translation="MGHAIDGGMLLLRVFLGALLASHGAQKLLGWFNGYGIEGTGGYF
                     EMIGYPRGKIMAVLAGLTEGLGGIGLAAGLLTPIVSLAFVGLFANVAVAGHGRSFWNH
                     NQPSGIEYPLVLGIGTGIFSLIGPGAFSVDRAFGWWLYGWGWFAASVTLGLLVGAGVL
                     AWRRPPTPAQEAEEDPSGRAAA"
     gene            7295..7906
                     /locus_tag="VE646_02865"
     CDS             7295..7906
                     /locus_tag="VE646_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZD78970.1"
                     /translation="MRTAKGEPSFPILGQEPSERADAARNRERVIAAAERLFEERGFR
                     DVSMDDVAETAGVGKGTVYRRFGDRAGLALALLGRAEALFQQRILTGPPPLGPGAPPE
                     ERLVVFLRALAGLVHRYLDLHVMAESGDPVTRFGSAIYAFYREHVRLLIAEIRPELDA
                     ACLADALLAPLDAGLMRHAADRDVSLRRMKDALERVARGALAV"
     gene            complement(7967..>8399)
                     /locus_tag="VE646_02870"
     CDS             complement(7967..>8399)
                     /locus_tag="VE646_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZD78971.1"
                     /translation="RGYRLDTTTRAMGMALGDVRLPRPRIELGAPDRSEYLRILGAPP
                     EFLARADPHAYHILLAGLRDENVATAMAFDHAGDRGIYNLGTIEHARRGGLGTALTAL
                     HVHDVLARGCRTASLQSTPMAEGIYAAVGFRDLGRILEYVP"
BASE COUNT         1144 a         2982 c         2978 g         1295 t
ORIGIN      
        1 cgatgctcgt ggaggggccg aagacgcact cgggcctcac ggacctgcgc gtgaccgacg
       61 ccggccacgt ggccgagggc cgctgacggg gaggcgtcag acgggccgcg ccgccggtgg
      121 gtggccgttc gagcgttcgg cctgagcctc gaggtccggc aggaccacca gcggctccga
      181 gccctcgccg ggggtggcac cctccgcgct gtcggcgcgg gcctgccaga gcttggcggc
      241 caggtcctcc accgtcgcct ccaggctccg gccgtgcgcc acttgacgca ggaacaggcg
      301 tcgtgaagtg gccagctcga cccggagctc gtccagcagc ccgtcccgtg ccgccagggt
      361 ggctcgagcc tcctgggccg agcgagccgc gacctccagc tcggcatcga gctccgcgca
      421 gcgagcctcc gccgcctggg tccgttgcgt gagcgcatcc gcgcgctgct cggcggcggc
      481 ggcgcgtccg gaagactcgc tcagctcacg ctcgcgggcc gcgagggaca gccggacgct
      541 ccgggccgag tagccgaaca gtccgcgtcg cagccgggga gcgttcatcg gggacctcct
      601 gtcgatcgcc aagcgccaca gggggattct ccgaccgttc gcggggtggt tcaaacaccg
      661 ccaacgggag gttgggtccg ctatcgagcg gcttcccgga tcaccccgcc cgcagccaga
      721 tcggtccccg ctcgctggtc ggatcgctgg gcctccggca cgatgatggg ccgtggtgcc
      781 cctgcctggg cgtgggccga ctggtcgccc gatgggatcg gcgacccccc ggggacgtcg
      841 ccgatatcgc ggagccttcg gttgagctcg tccagagcgc tcgcagccgg ttccaggacc
      901 tcgcggatct gctccgcgag gtcggcggtc cgtctgcgcg tctccctcag ccaccccccg
      961 aggtccccca gggtgggctc ggcgctcctt cgccatgcca tcagctggtc ggtctccatc
     1021 cggacctgct gctgtacccg ttcgagctct tccagctcct cctcgccctt gagccgggcg
     1081 gattccacga tcttcgtgac ggccatctcg gcggctcgga gcgcaccagc cacctcttcg
     1141 gtcaggggat ccgcctcctg gagcgacacg gtcacgtccg cccccccggg ctgaccgccc
     1201 agggcctcga tctccgtccg caggcggtcc cggtcggccc tgacccgatc gagctccccg
     1261 ctcagctgca ccacccgatc gcgcaaggcg tcccgttccg ggcccaggaa cctcatccga
     1321 gcttccagct cggagacacg gagctcggcc gcctgcagcc ggccggtgta gtcgccgacc
     1381 tcgttgcggg cggtgcggag cgccagatcc aggcgggccg ccctggcttc gacgtcgagc
     1441 acccgtcgct catctcgggt cgcgtcccgc agcttggcct ggagcgatgc gatctgggct
     1501 tcagcagctt cggcccgctc cgtgagctcg gccacgtgcc gtcgggcgtc ttcgagctct
     1561 gcgtggagcc gctccgcatg gtcgagagcg tcccgcgcct gcctggacgc cctccgggcg
     1621 atctggtcac ggttcgacag gagctcgtgc acgctccggc gcgagtaccc cacgcctcgt
     1681 ttcaggggag gcaggtccaa gcggtcgggc ggccatgcgt cgcccgggcg atcggtgccc
     1741 ccaccggagg tcatgacttc gggacctccc tcagcgggtc cgatcccccg gcccccagcg
     1801 gtggcccatc ggtggatgcc tccgtggtcg ctgtcccatc gtcgccccgg aggtccaggc
     1861 cgccgccttc gccgccttcc ggtccgccga acgcacctgc ctccggcgcg cctgcctcgg
     1921 gcgcacccag ctcgggcgca tcgccttcat ccggttccgg tccgccggac gcaccgggtt
     1981 cgggggagcc ctggggtccc gtttccccgg cctcgttcag tccctccagc tgttccccca
     2041 gcacctgcat ggcggcggtg atgggatgaa ccgcctcctg gacccgctcg ccgaaccgca
     2101 tcgcgtggag ctgtgcgtcc tcgatcgcgc tcctgatcac ctcgacctgc ggtgccaggc
     2161 gatctcgcca ctgagcgaga cggtcgatat cggctcggac ctgatccctg gctcgctcgg
     2221 cctcctgcag ttcccgatgg acctcctcca gttcctgctg gaccctggca cgtcctccct
     2281 ccatgaccct ggccacgacc tcttcggccg cgcgcagcac gtcggtgacc tcgtcggggg
     2341 tcgaggggtc ggagatcgac ggagtgatgt ccatcctggc ctgcgcggcc tccagctccg
     2401 aggtcagctc ttcgatcctg gattcggtgc tgctggcacg ctcgctctcg ctgacgaggt
     2461 cgcggcgggc ggtgagcagg tcctggcgaa gctcgtccag ctccgccacg tactggtcgg
     2521 ctcggtgttc ggcggcctgc cggcggcgtt cctcgtcggc cagaccgctc cgcgcttgtt
     2581 ccagctcccc ccgggaccgg gtcaggtcgt cctgcgcctg ctcgagctgt gtccggacct
     2641 cggcgagcgc ccgctcccgg tcctcgacgt actctgggcg gggcatgttg gcccgcacgg
     2701 tctccaactc acgctcgagg tcggcgatac gggcctcagc gatcttcgcg ctctccgagg
     2761 cgaccgccaa catctgttcg cgatcggcca acatctgttc gcgatcggcg aggagcttct
     2821 gcacgtcctc cggtgaatag cccatgaatc gtcgcttcag ctccggctcc tccgccggat
     2881 actctcgttc ttccataggg tctggttccc tcccggctcg ggcaggccgt gcaacgatta
     2941 ttcgacctct ggctgaggac ttcaatcacc tggaagagcc gcggtcagct ggaagagccg
     3001 cggcacccca tctggcctac atcatcgcga tcttccgcgg tgacggggcg ctgtcgaggc
     3061 tctccgccaa cgggacccgc ggggtgcggc acggccacag gcggtccggc gccaaggcca
     3121 catcgtggta ccttccaggc atgtaggtgg tcccgacgcc caccccgggt gggcgtctcg
     3181 tgctatctgg gggagaggat ggagatcgag atcggcgccg gcaaggcggc cagacgcgcg
     3241 tacgggttcg acgaggtggc gatcgtgccc tcgcggcgga cccgggaccc cgacgacgtc
     3301 gacatctcct gggagatcga tgcctaccgg ttcgacctgc cgatgatggc cgcggccatg
     3361 gacgcggcgg tctcgccggc caccgcgatc gaggtcggca ggctgggcgg actggcggtc
     3421 atgaacctgg aagggctcca gacccggttc gaggaccccg accccgcctt cgaggagatc
     3481 gcccagctgc cggacgagaa ggccacccgc cggatgcagg agctctacag ggagccggtg
     3541 aaggacgagc tgatcacccg gcggatccga gagatcaagg aggccggggt catcgccgcc
     3601 gcctcgctca ccccccaacg ggtggggcgg tacgcgaagc tggtcctgga cgctgagctg
     3661 gacatcctgg tgatccaggg gaccgtggtc tcggcggagc acgtctcctc ccgcaccgag
     3721 ccgctgaacc tgaaagagtt catcgccggg ttcgatctgc cggtgatcgt ggggggctgc
     3781 gcctcctacc agaccgccct gcacctgatg cgtaccggcg cggtgggtgt gctggtgggc
     3841 gtgggccccg ggaacgcttg caccacgcgc ggcgtgatcg gcgtcggtgt gccccaggcc
     3901 accgccatcg cggacgcctc ggcggcgcgt tcggagcacc tgcgcgagac cggccgctac
     3961 tgccacgtga tcgccgacgg cggcatgcgg accggcgggg acatcgccaa ggccatcgcg
     4021 tgcggggccg acgccgtgat gatcggctcg cccctcaccc gggcctacga ggcgccgggg
     4081 cgcggctatc actgggggat ggccaccttc catcccaccc tgccccgggg ggcccgggtg
     4141 aagacccagc aggtggcctc gctggaggag atcctggtgg ggccggcgca cgagaacgac
     4201 gggacgttca acctgttcgg cgccctggcc acctccatgg ccaccaccgg ctacgccacg
     4261 atcaaggagt tccagaaggc cgagctggtg gtggcaccct cgatcaagac cgagggcaag
     4321 acctaccagc aggcccagcg cgtggggatg ggccactgag ccggagccgg tctggggacg
     4381 tccctctcca ttgacggcga tatggctcca gcgtatggtt ttctggatga agacgaccct
     4441 gaacctcgac gacgatctga tgcggcaggc gaagaagcgt gcggtggagg aaggaacgac
     4501 actgacccac gtggtggagg aggcgcttcg ggccgcgctc gcgacgcgag ggcggtcgcc
     4561 gcggcgcacc gtccggtggg tggtggtcga cggcggcccc ccccgcgtcg atgtggcgga
     4621 ccgcgacgcg ctctacgacg tgatggagcg gccggactga tcgccatcga cacgaacatc
     4681 ctggtctacg cccaccgccg ccaggcgccg cagcaccaac gcgcgctcga agctctccgc
     4741 cagctggtcg tgggacccgc agcctggggg gtgccggtct tcgtggccgg tgagttcctg
     4801 cgcgtggtga cccatccgag gctcttcgat cgcccgtcga agctggagga cgcgctcgcc
     4861 gcgctcgacg gactcctttc cagcggaacc gctcgactgc tccttcccgg ggagcggtac
     4921 tggtcgatcc tgcgggagac cgcgctcgac ggccgggcga gcgggaacct gctgttcgac
     4981 gcgcagatcg cagccgtctg catcgagcac ggcgtgacca cgatcatgac cgaggatcgc
     5041 gacttccgcc ggttctcgcg catgcaggtc ctgccgctgc tttgagcctg ctaccgttcg
     5101 cccatggctg acgcgcggcc ggtcctggtc gtcgatctcg gtgcccagta cgcgcagctg
     5161 atcgcgcgcc gggtgcggga gtgccacgtg tattccgagg tcgtcccgca cgacgtctcg
     5221 gcccaggacc tgagagccag ggggccagcg gggatcatcc tgtcgggcgg accggcgtcc
     5281 gtgtacgagg cgggggcgcc ggacgtggat ccggagctgt tccatctggg cgtcccggtc
     5341 ctcgggatct gctacgggca tcagctcatg agccgggccc tgggcggcga ggtggctgcc
     5401 accgggatcc gggagtacgg gcgcacggag ctggccgtgg acgagcccgg cgtcctgctc
     5461 gaggatctgc cgccccggga cacggtctgg atgagccacg gcgatgccgt gagcgcagcc
     5521 ccgcagggct tccgggtcac cgcccacacg acctccacac cggtcgcggc gatggaggat
     5581 cgccagcgcg gcctgttcgg cgtgcagttc caccccgagg tggcgcacac gccgaagggc
     5641 atggacgtga tgaagcggtt cctctacgac ggctgcgggc tgctgcccga ctggacgccg
     5701 gcgaacgtga tcgagaggtc ggtggcccgg atccgccacc aggtggggga ccgcaaggtg
     5761 atctgcgccc tgtcgggcgg cgtggactcc gcggtggccg ccttgctggt gtacaaggcg
     5821 gtcggcgagc agctcacctg cgtgttcgtc gacaccggcc tgctgcgcga gggcgagccg
     5881 gagcaggtgg aggaggcctt cggccgccac ttccgtgtgc ccctggtgca cgtgaaggcc
     5941 gcggagcggt tcctggaccg gctggccggg gtcaccgacc ccgaggacaa gcgcaagcgg
     6001 atcggcgagg agttcatccg ggtgttcgag gaggcggccg caccgatcgg cgccggactg
     6061 ctggtccagg gcacgctgta cccggacgtg atcgagtcgg gctctcgcac ggcggccacg
     6121 atcaagagcc accacaacgt gggcggcctg cccgaggacc tctccttcga gctggtggag
     6181 ccgctgcggg acctgttcaa ggacgaggtg cggcgggtgg gctcggagct cgggctgccc
     6241 gaggagatcg tctggcgtca gcccttcccg ggcccggggc tggcggtgcg gatcgtgggc
     6301 gaggtgacgg ccgagcgcct cgagatggtc cgggcggggg atgcgatcgt ccgcgaggag
     6361 atccgccggg ccggcctgga gcgcccgatc tggcaggcct tcgcggtgtt gctgggcgag
     6421 gtccgaagcg tgggcgtgat gggcgacggg cggacctacc agcaccccgt ggtggtccgg
     6481 gccgtgacca gcgaggacgc catgaccgcc gactgggccc gcctgccggc cgaggtgctc
     6541 gaccgcatcg cgacccgggt gatcggcgag gttcggggcg tcaaccgggt cgcctacgac
     6601 gtcacctcca agccgcccgg cacgatcgag tgggagtgag ccgtcaccgc cggcctacgc
     6661 ggcggcccgc ccgctggggt cctcctcagc ctcctgggcc ggggtaggag gtcgtcgcca
     6721 cgccaggaca cccgctccca cgagcaagcc aagcgtcacc gaggccgcga accatcccca
     6781 tccgtacagc caccatccga aggcgcggtc caccgagaag gcaccgggac cgatcaggga
     6841 gaagatcccc gtcccgatcc ccagcacgag cgggtactcg atcccgctcg gctggttgtg
     6901 gttccagaag ctccgcccgt gacccgcgac ggcgacgttg gcgaacagcc ccacgaaggc
     6961 caaggacacg atcggcgtga gcagccccgc cgcgagcccg atgccaccga gcccctcggt
     7021 gagcccggcc aggacggcca tgatcttgcc cctggggtag ccgatcatct cgaagtagcc
     7081 cccggtgccc tcgatgccgt agccgttgaa ccacccgagg agcttctgcg ctccgtgcga
     7141 ggcgagcagc gccccgagga acaccctcag gagcagcatc ccaccgtcga tcgcgtgccc
     7201 catcctcgat ccctccttcc gcggccccac atcgccctcg atgtgatcgg cgccccttcc
     7261 tggaaccgga cttcggtccg gttatgctcg atcagtgaga acggcgaagg gggagccttc
     7321 attcccgatc ctggggcagg aaccgtcgga gcgcgccgac gccgcccgca atcgcgagcg
     7381 ggtgatcgcc gcggcggagc gcctgttcga ggaacggggg ttccgggacg tctccatgga
     7441 cgatgtggcc gagacggcag gggtgggcaa ggggacggtc taccggcgct tcggggaccg
     7501 cgccgggctc gcccttgcgc tcctcggtcg agccgaggcc ctgttccagc agcggatctt
     7561 gaccgggccg ccgccgctgg gaccgggcgc gccacccgag gagcggctgg tcgtgttcct
     7621 acgcgcgctc gccggcctgg tgcaccggta cctggacctg catgtcatgg ccgagtccgg
     7681 ggacccggtc actcggttcg ggagcgccat ctacgccttc taccgggagc acgtgcgtct
     7741 gctgatcgct gagatccgcc ccgagctcga cgctgcgtgc ctggcggacg cgctgctcgc
     7801 cccgctggac gcggggctca tgcggcacgc cgcggaccgc gacgtctcgc tccgacggat
     7861 gaaggatgcc ctcgaacgcg tggcgcgcgg cgccttggcg gtctgaggtg cgggtgggtg
     7921 aagccctcgc atgggtcgct ccggtagcag accagcgtcg acgggcctac ggaacgtatt
     7981 cgaggatccg tcccagatcg cggaagccga cggccgcgta gatgccttcg gccatcggcg
     8041 tggactgcag actggcggtt cgacatccgc gcgcgagcac gtcgtgaacg tggagcgccg
     8101 tcagcgccgt accgagaccg cctcgccgtg cgtgctcgat cgtgcccagg ttgtagatcc
     8161 cgcgatcgcc ggcgtgatcg aaggccatgg ccgtcgcgac gttctcgtcg cggaggcccg
     8221 ccagcaggat gtggtaggcg tgggggtcgg cacgggcgag gaactccggt ggcgctccga
     8281 ggatgcgcag gtactcggac cgatctggcg ccccgagctc gatccgcggc cgaggcaggc
     8341 ggacgtcccc gagcgccatg cccatggcgc gggtcgtggt gtcgagcctg tagccgcga
//
LOCUS       DASQQQ010000065         5025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_253560, whole genome shotgun sequence.
ACCESSION   DASQQQ010000065 DASQQQ010000000
VERSION     DASQQQ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253560"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VE646_02875"
     CDS             <1..381
                     /locus_tag="VE646_02875"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HZD78972.1"
                     /translation="GGACEIAIHARKVFVIMRQAQRSFVDRLHFRTSPGHSGDPEHDR
                     QRGWTGSGPTSVVTDLGTYTFDDASGEMTLATLHPGVSLDEVRQNMGWEPKVAPELGR
                     TPEPTPDELRLLREELDPRGVYTK"
     gene            393..1709
                     /locus_tag="VE646_02880"
     CDS             393..1709
                     /locus_tag="VE646_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZD78973.1"
                     /translation="MGHVFHRVLTRELPTAVRAEGVWIEDAAGRRYLDGAGGAIVVNL
                     GHGDHVVVEAMAEQSGRLPYVHGTMFTTDALEAYADDLAPLLPMDHPRIYPVSGGSEA
                     VETAMKLARTYHLARGDEERSVVIGRRASYHGNTLGALDVGGKEALKRPYLPWLGRFP
                     QVAAAYEYRCSMPDHPLACGERHAEELDRTIREIGPRRVAGFIAEPVAGATLAAAVPT
                     DDYWPAMADVCRRHGVLLIADEVMTGFGRTGRWFGMDHWGIRPDILTCGKGSTSGYWP
                     FGFAAASGEVFQTVRGRGFVHGFTWSHNGVGAATAHAVLRRLRDGDLVEASRVQGERL
                     LKDLSSALEDVPIVGDVRGIGLMIGIELVADRETQGPFARAKQVTERVVAAAKELGLL
                     LYSSTGHVDGRDGDLVMLGPPFVISDQECALVVQWTREALTAVAAT"
     gene            1713..2861
                     /locus_tag="VE646_02885"
     CDS             1713..2861
                     /locus_tag="VE646_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HZD78974.1"
                     /translation="MSDRVALVRCPEARIYDHGPQHPLRPQRVLYTWDLIDACGLPEL
                     PNVIEVPCRPATDDELGRVHTDPFIDATRRAGHGEEGDWWRYGYGPGDNPIFPDMHEA
                     GALVAGASLVAAEVVHHGEAEHAFSAAGGLHHAMPAKASGFCVYDDPAIAITWLLDQG
                     VEPIAYVDVDVHHGDGVQAIFYDDPRVLTVSIHESGEFLFPGSGFVTERGLAGAEGTK
                     VNVPLPPRTTDEAWLTALREIVPSVLRAWKPKVLVTQLGCDSHHTDPLAHLRLTTRAY
                     REAAALLHALAHQTAGGRWVATGGGGYQWARVVPRAWTLYFGEMCGHQLPDAVPEAWI
                     EKVEFDIRSWVPTTFSEDPVPNDVLDPETESVIRQVKATCFPFFGLPV"
     gene            2921..4339
                     /gene="pyk"
                     /locus_tag="VE646_02890"
     CDS             2921..4339
                     /gene="pyk"
                     /locus_tag="VE646_02890"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HZD78975.1"
                     /translation="MPRRAKLVCTLGPSTATEPSVRALAEARTDVFRVNFSHGDPASH
                     AELVRLVRSLAEALGRDLTVMADLPGPKIRLGELTEGSLHLDAEHPFVLRADGAPGDV
                     SGAPTTYAGLADDVREGDRVLLADGAVELVVRATRGRDVITEVVRAGSVRARAGVNVP
                     AERLGLPAVTERDREGLARALDLGVDLVAQSFVRRAEDVRTLRELMGDRSVPIVAKIE
                     TRPAIEAIDAVLDEADALMVARGDLGVELPMEQIPVLQKELLVRARSTGKPAVVATQM
                     LESMIHAPRPTRAEASDVANAVLDGADAIMLSGETAIGEYPVEAARAAARIAEVAEER
                     AGRFRTPGEPCRHTEEAAAVAHAAAQVAAGDPDVVAIACFTGTGRTAELLASERPGVP
                     IYAFSSHQRVRRILGLRWGVTSLAAVSPNDTDEMIAVMDAGLVRAGLVRAGQSVVMAA
                     SSPAGKTHTNMLKVHHIGSAVR"
     gene            4622..4822
                     /locus_tag="VE646_02895"
     CDS             4622..4822
                     /locus_tag="VE646_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78976.1"
                     /translation="MPFPPDEPIFGEELERVDEESAEHAEEHRREHEAEHEDDRSTED
                     DRSTKDRSGTAVSWWRRLFRRM"
     gene            complement(4841..>5025)
                     /locus_tag="VE646_02900"
     CDS             complement(4841..>5025)
                     /locus_tag="VE646_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78977.1"
                     /translation="VMEADSVARTIVDVVREGRGPEVNVPRGLAPLQVVRVVAPPLYR
                     RGVRRLMRRFRATPAR"
BASE COUNT          713 a         1753 c         1870 g          689 t
ORIGIN      
        1 ggcggggcgt gcgagatcgc gatccacgcg cgcaaggtct tcgtgatcat gcggcaggcc
       61 caacgctcgt tcgtggaccg gctgcacttc cgcaccagcc cgggccactc gggcgatccc
      121 gagcacgacc ggcagcgcgg ctggaccggc agcggcccca cctcggtggt cacggacctg
      181 ggcacctaca ccttcgacga cgccagcggc gagatgacgc tggccaccct gcacccgggg
      241 gtgagcctgg acgaggtccg tcagaacatg ggctgggagc cgaaggtggc gcccgagctc
      301 ggacgcacgc cggagccgac cccggacgag ctccggctcc tgcgcgagga gctggatcct
      361 cggggcgtgt acaccaagtg aggagcgatg ccatgggcca cgtcttccac cgggtgctca
      421 cccgcgagct gcccaccgcc gtccgggccg agggcgtctg gatcgaggac gcggcggggc
      481 gacgctacct ggatggggcc ggcggcgcca tcgtggtgaa cctggggcac ggcgaccacg
      541 tcgtggtgga ggccatggcc gagcagtccg ggcggctgcc gtacgtgcac ggcacgatgt
      601 tcaccacgga cgccttggag gcctacgcgg acgacctggc gccgctcctg ccgatggacc
      661 atccgcggat ctacccggtg tcgggcggca gcgaggccgt ggagacggcc atgaagctcg
      721 cgcgcacgta ccacctggcc cgaggcgacg aggagcgttc cgtcgtgatc ggccggcgcg
      781 cctcgtacca cgggaacacc ctgggggcgc tggacgtcgg gggcaaggag gcgctgaagc
      841 ggccctacct cccctggctc gggcggttcc ctcaggtcgc ggcggcgtac gagtaccggt
      901 gctcgatgcc ggatcacccc ctggcctgcg gggagcgcca tgcggaggag ctcgaccgca
      961 ccatccgaga gatcggaccg cggcgggtgg ccgggttcat cgcagagccg gtggcgggcg
     1021 ccacgctggc cgcggccgtc cccaccgacg actactggcc ggcgatggcc gatgtctgcc
     1081 gccgacacgg cgtgctgctg atcgcggacg aggtcatgac cgggttcggc cggaccgggc
     1141 ggtggttcgg gatggatcac tgggggatcc ggccggacat cctcacctgc ggcaagggct
     1201 cgaccagcgg gtactggccc ttcgggttcg ccgccgccag tggggaggtc ttccagacgg
     1261 tccgcggccg cggcttcgtg cacgggttca cctggtcgca caacggcgtg ggggcggcca
     1321 ccgcgcacgc cgtgctgcgg cggctccgag acggcgatct ggtcgaggcc tcgcgcgtgc
     1381 agggggagcg gctgttgaag gatctgagct cggccctgga ggacgtgccg atcgtgggcg
     1441 acgtccgcgg catcggcctg atgatcggga tcgagctggt ggccgatcgg gagacccagg
     1501 ggcccttcgc ccgggccaag caggtgaccg agcgggtggt ggccgcggcc aaggagctcg
     1561 ggctcctcct gtactcgagc accggccacg tggacggcag ggatggcgac ctggtgatgc
     1621 tcggaccacc gttcgtgatc tccgaccagg agtgcgccct ggtggtgcag tggacgcgcg
     1681 aggccctgac cgccgtggcg gcgacctgac cggtgagtga ccgggtcgcc ctcgtccggt
     1741 gccccgaggc caggatctac gaccacggac cccagcaccc gctccgcccc cagcgcgtcc
     1801 tgtacacctg ggacctgatc gacgcctgtg ggctccccga gctcccgaac gtgatcgagg
     1861 ttccctgccg cccggccacc gacgacgagc tcgggagggt gcataccgac cccttcatcg
     1921 acgccaccag gcgggccggt cacggtgagg aaggggactg gtggcggtac ggctacgggc
     1981 ccggtgacaa tcccatcttc cccgacatgc acgaggccgg cgcgctggtg gccggggcgt
     2041 cgctggtcgc cgccgaggtc gtgcaccacg gggaggccga gcacgccttc agcgccgccg
     2101 ggggcctgca ccacgcgatg cccgccaagg cctcggggtt ctgcgtctac gacgacccgg
     2161 cgatcgccat cacgtggctg ttggaccagg gggtcgagcc gatcgcgtac gtggacgtgg
     2221 atgtgcatca cggcgacggg gtgcaggcca tcttctacga cgaccctcgc gtgctgaccg
     2281 tctcgatcca cgagtccggc gagttcctgt tccccggatc cgggttcgtc accgaacggg
     2341 gactggccgg cgcggaaggg acgaaggtga acgtgcctct gcctcccagg accaccgacg
     2401 aggcctggct cacggcgctg cgcgagatcg tcccctcggt cctccgagcc tggaagccga
     2461 aggtgctggt cacgcagctc gggtgcgact cccaccacac cgatccgctc gcgcacctcc
     2521 gcctgaccac cagagcgtac cgggaagccg cggcgctcct gcacgccctc gcgcatcaga
     2581 cggcgggcgg ccggtgggtg gccaccgggg gcggcggcta tcagtgggcc cgcgtggtcc
     2641 ctcgggcctg gaccctgtac ttcggcgaga tgtgcgggca ccagctgccc gacgccgtgc
     2701 cggaggcctg gatcgagaag gtggagttcg acatccggtc ctgggtgccc accacgttca
     2761 gcgaggatcc ggtgccgaac gacgtgctgg accccgagac cgagtccgtg atccggcagg
     2821 tcaaggccac ctgtttcccc ttcttcgggc tgcccgtctg atcctggcct ggccgcgcgt
     2881 ggccccggga ggcggggaac gcccgggtat cctcgaaggg atgcctcgac gggccaagct
     2941 cgtgtgcacc ctcgggccct ccaccgccac ggagccctcc gttcgggccc tggcggaggc
     3001 gaggacggac gtcttccggg tcaacttctc gcacggcgac cccgcctcac atgcggagct
     3061 cgtccgcctg gtccgctcgc tggccgaagc gctcgggcgc gacctgacgg tcatggccga
     3121 ccttcccggt ccgaagatcc gcttgggcga actgaccgag gggtcgctcc acctcgacgc
     3181 cgagcatccc ttcgtgcttc gcgcggacgg tgctccgggc gacgtgtccg gggcccccac
     3241 gacctacgcg ggcctggccg acgacgtccg cgagggcgat cgggtcctgt tggccgacgg
     3301 ggcggtggag ctcgtggtcc gcgccacgcg ggggcgggac gtgatcaccg aggtcgtgcg
     3361 ggccggctcg gtgcgggcgc gggccggcgt gaacgtaccg gccgagcggc tggggctgcc
     3421 cgccgtcacc gaacgggacc gggaggggct ggcgcgagcg ctcgacctcg gcgtcgacct
     3481 ggtggcgcag tcattcgtcc gtcgagccga ggacgtccgc acgctccgcg agctcatggg
     3541 cgaccggtcg gtgcccatcg tcgcgaagat cgagacccgc ccggcgatcg aggcgatcga
     3601 tgccgtgctg gacgaggcgg acgccctgat ggtggcccgc ggtgatctcg gcgtggagct
     3661 gcccatggag cagatcccgg tcctgcagaa ggagctgctg gtgagggccc ggtcgacggg
     3721 caagccggcg gtcgtcgcga cccagatgct ggagtcgatg atccacgcgc cccgtcccac
     3781 gagggccgag gcaagcgacg tggccaacgc cgtcctcgac ggtgccgacg ccatcatgct
     3841 ctcgggggag accgccatcg gcgagtaccc ggtggaagcg gcgcgtgcgg cggcccggat
     3901 cgccgaggtg gccgaggaac gcgccggccg gttccgaacc cccggcgagc cctgccggca
     3961 cacggaggag gcggcggcgg tggctcatgc ggcggcacag gtggcggccg gtgaccccga
     4021 cgtcgtggcg atcgcctgct tcacggggac ggggcggacc gccgagctgc tggcctccga
     4081 gcgtcccggg gtgccgatct acgcgttctc ctcccatcag cgggtccggc ggatcctcgg
     4141 gctgcggtgg ggggtgacga gcctcgcggc cgtctccccg aacgacaccg acgagatgat
     4201 cgccgtgatg gatgccggac tggtccgcgc cggtctggtc cgcgccggtc agtcggtggt
     4261 gatggccgcc tcctcaccgg cgggcaagac ccacacgaac atgttgaagg tccaccacat
     4321 cggcagcgcc gtccgctgac ggcgagcgtg tgctctcggt gagagggagg agcggccatg
     4381 ggctacacgc ctgagcagcc acggatcccc aaaggccctc gagccggaga ggccgaggga
     4441 ccgtgaccgc gaatgggttg ccgaggatcg cgcggagcgt gagatgcgcg agcaccaggg
     4501 gtccgcgctc cgacgcctgt tcaggcgctt cgtcggaagg cggtagtcgg caacgcagac
     4561 gagcttccca tcgcccgggt cggcgttccg tgctcgctcg gcatagagtg aggtcatggg
     4621 catgccgttc ccacccgacg agccgatctt cggcgaggag ctcgaacggg tcgacgagga
     4681 gtctgccgag cacgcggagg agcaccgccg cgagcacgag gccgagcacg aggacgaccg
     4741 ctccaccgag gacgaccgtt ccacgaagga ccggtccggc acggccgtct cctggtggcg
     4801 tcggctgttc cgccgcatgt agcccgggca gcgagcgctg ctatcgagcc ggcgtcgcgc
     4861 ggaaccgccg catcagtcgc cgcacgcccc gccggtacag tggcggggcg accacgcgca
     4921 cgacctgcag gggggcgagc ccacggggca cgttcacctc gggaccccgg ccctcccgca
     4981 ccacgtccac gatggtcctg gccaccgagt ccgcctccat cacca
//
LOCUS       DASQQQ010000066         5483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_305741, whole genome shotgun sequence.
ACCESSION   DASQQQ010000066 DASQQQ010000000
VERSION     DASQQQ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305741"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VE646_02905"
     CDS             complement(<1..820)
                     /locus_tag="VE646_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZD78978.1"
                     /translation="MTPAGILVDHMMYGVVVALVVRGDRLSDDRWPAVGSPVSPRTCG
                     KHGWPSGVVLGMGILPTARGRADDIVREAAWLARCVGRGDWAPLSETDLAELGNRLVH
                     EELLPGTALFAQGATSEAVWIVREGRVELSRRDGRRRTILKIVHPGEVDGDISLILAM
                     PLPYSAHAIDRVRALRLPASDFEWLIASRPALARRWLSSVAGRLVHAERRILQLSGKD
                     LRHQVSSLLLDEANGTTVELPQASLAALLGVRRQSLNKVLRDLERTGVIALSYRR"
     gene            complement(950..2776)
                     /locus_tag="VE646_02910"
     CDS             complement(950..2776)
                     /locus_tag="VE646_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HZD78979.1"
                     /translation="MSRTPIGEHGFLADCHGSALVSANGSLDWLCFDRFDAAPVFSRI
                     LDDAAGHWSIAPVDEAEVSRRYIAGTLVLETIFRTASGILVLEDACALGEWAGEHEIG
                     SEAPGVLVRRVRCEAGPVRVGIEYAPRPEFGLISPLLTRIDGGLRARGGASLLVLSGG
                     SDMEIDGPSARLVVEMVPGQSRTFALAHAWSWEEEPQVLDEGAAGGLIERSVATWRRW
                     SAAHDRYEGPWRELVVHSGRVLQGLTFRPTGAIVAAPTTSLPEQIGGQRNWDYRYAWV
                     RDASLTLQALRRTACQVEAQRFFTWMSGAAAESLHRDIELQIVFGIGGEHDLSERELP
                     HLRGYRGSRPVRVGNGAWGQRQLDVYGELLDAASVHVERFEELDGPTRHFLKDAADAA
                     AERWREDDEGIWEVRGGRRPFLHSKLMCWVALDRALAMAHELDARERVEDWTRTRGEI
                     RRAILEHGWSDAAGAFTQSFGSEDLDASVLMMPIVGFLQADDPRMLATIDAIAERLVD
                     ERGLVYRYRSHDGLDGEEGTFLLCTFWLAQAQAMAGRLEGARETFERAIAHVNDVGLL
                     SEEIDGSTGELLGNFPQAFSHIGLINAAAAIHEAEQLRAQRS"
     gene            complement(2784..3506)
                     /locus_tag="VE646_02915"
     CDS             complement(2784..3506)
                     /locus_tag="VE646_02915"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HZD78980.1"
                     /translation="MAAHRVGSLIATVASSSIQALTAEQMREVDRVAVEQLGLLLIQM
                     MENAGARLAELALARFGPGTAVVLAGRGGNGGGGLVAARHLVNRGVRVQVTLAEGPDR
                     LAEVPRHQLRTLEQMGVPIAFRPIEADLVLDALLGYALRGDPHGGATRLIRWANEQGA
                     PVCALDVPSGLEASTGRVGDPCVRATATLTLALPKTGLGAAPHVVGELYLADIGVPDA
                     GCAKIGVTVGPIFRPSAILRLV"
     gene            3724..>5483
                     /locus_tag="VE646_02920"
     CDS             3724..>5483
                     /locus_tag="VE646_02920"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZD78981.1"
                     /translation="MAAPVRTRGVPAEGTGPSPPGRRYRYVAWVLWLLAVLVYLGAMV
                     AGGLTGSMSSQGAVDVVLGMSSVLGAVAYATAGLLIVSRHPGQAVGWLLMAVPLSFGG
                     ANLVGGLATYGLATRPGSIPFARALAWIDRWSLLPILGLIVLLVLLFPEGRIPSRRWR
                     PVLWLAVGAPLAAAAGFALTPGRMTGSLADLTSVVVANPLGIDALAAPIGALTVAASL
                     GSVAAGLLAAGSMVVRYRSSKAEVRQQIRWMALVAAIFLVAFLLEYPVFFLSGGADQG
                     LGSVLSNVLFAVMALAIILGIPAACAIAILKYRLYDLDVVVRKTLVVGSLAVFFTVVY
                     AGIVAGLGAVVGSRFDSTASFLAAAAVAVVFAPARDRARRLADRLVYGRRATPYEILT
                     EFSSHMASTEASTDVLSRMAEVLGTGTGARRARVWLRVGRELSPAAAWPSDAPAVPAA
                     TLAADGLPEVPDQAVFEVRHRGELLGAISVSMPESDPLTPAKEKLARDFAAQASLALR
                     NVRLIEELRASRQRLVAAQDEERRRLERNIHGGAQQQLVALAVKLGLAEGLIDQDPVE
                     AREMIQALRAASQEALEDLR"
BASE COUNT          761 a         2042 c         1854 g          826 t
ORIGIN      
        1 cccgccggta ggacagggcg atcacacccg tccgctcgag gtctcggagc accttgttca
       61 gggactgtcg cctcacgcct aacagcgccg ccagcgatgc ctggggcagc tcgaccgtgg
      121 tgccgttcgc ctcgtccagg agcagactcg agacctggtg acgcaggtcc ttgcccgaga
      181 gctgcaggat ccgccgctcg gcgtggacga gcctccccgc gacgctggag agccaccggc
      241 gcgccagcgc cggacggctc gcgatcagcc attcgaagtc gctcgccggc aaccgcagcg
      301 cgcggacccg gtcgatcgcg tgcgccgaat acgggagcgg catcgccagg atcaggctga
      361 tgtcgccgtc gacctcgccc ggatggacga tcttcaggat cgtgcggcgc cggccgtccc
      421 gccgactgag ctccacccgc ccctcccgca cgatccagac ggcctccgag gtcgcaccct
      481 gcgcgaacag cgcggtcccc ggcaggagct cctcgtggac cagtcggttg ccgagctcgg
      541 ccaggtccgt ctcggacagc ggcgcccagt cgccgcgacc gacgcagcgc gccagccacg
      601 cggcctcccg gactatgtca tcggcgcgac ctcgagccgt cggcaggatg cccatgccga
      661 gcacaacccc gctcggccac ccgtgcttcc cgcaggtgcg tggggatacc ggcgacccca
      721 cggccggcca gcggtcgtcg ctcagacgat cgccgcgtac caccagcgcc acgaccacgc
      781 cgtacatcat gtgatccacg aggatgccgg ctggggtcat gccacctcac cccggtgccg
      841 gctgcgcccg ggcttgcgat ccgaagcccg acgcccggac cggggacctg gtgctcccgt
      901 catggcgggc tcttcgccgt ccgtaccctg atgtgaccct gtgaaccgat caggaccgct
      961 gggctcgcag ctgctctgct tcgtggatcg ctgcggcagc gttgatcagg ccgatgtggc
     1021 tgaaggcttg cgggaagttg cccagcagct cgcccgtgga cccatcgatc tcctcggaca
     1081 gcaggcctac gtcgttcacg tgggcgatgg cccgctcgaa ggtctcgcgg gccccctcca
     1141 gtcggcccgc catcgcctgg gcctgagcga gccagaacgt gcagagcagg aacgttccct
     1201 cctcgccgtc caggccgtcg tgcgagcggt accggtacac gagccctcgc tcgtcgacca
     1261 gtcgttcggc gatcgcgtcg atcgtggcca gcatccgggg atcgtccgcc tgcaggaagc
     1321 ccacgatcgg catcatgagg actgaggcgt ccaggtcctc agacccgaac gactgcgtga
     1381 aagcgcccgc tgcatcgctc catccgtgct cgaggatcgc cctccggatc tccccacgcg
     1441 tccgggtcca gtcctccacg cgctcgcgtg catcgagctc atgggccatc gccagggccc
     1501 ggtccagggc cacccagcac atcagcttgg agtgcaggaa cggccgcctg cccccccgga
     1561 cctcccagat cccctcgtca tcttcccgcc atcgctcggc cgcggcgtcc gcggcgtcct
     1621 tgaggaagtg cctggtcgga ccgtccagct cctcgaaccg ctccacgtgg acggaggccg
     1681 catccagcag ctcgccgtac acgtcgagct gtcgctgacc ccaggcgccg ttgccgaccc
     1741 gcaccggccg gctgccccgg tatccgcgca ggtgtggcag ctcgcgctcg gacagatcgt
     1801 gctcgccgcc gatgccgaac acgatctgta gctcgatgtc ccgatgcagg ctctccgcgg
     1861 cggcccccga catccaggtg aagaaccgct gcgcctccac ctggcaggcc gtgcggcgca
     1921 gagcctggag cgtgaggctc gcatcgcgca cccaggcgta tcggtagtcc cagttgcgct
     1981 ggccgccgat ctgctcgggc agcgaggtgg taggcgcggc gacgatcgca ccggtgggcc
     2041 ggaaggtgag gccctgcagc acccggccgc tgtgcaccac cagctcgcgc caggggccct
     2101 cgtaccggtc atgggccgcg gaccagcgac gccaggtcgc taccgagcgc tcgatcagac
     2161 caccggcggc accttcgtcc aggacctgag gctcctcctc ccagctccag gcgtgcgcga
     2221 gggcgaaggt ccgggactgc ccaggaacca tctcgaccac gagccgcgcg gaaggcccat
     2281 cgatctccat gtcgctccct cccgagagca ccagcaggct cgcacctccc cgggctcgca
     2341 gaccaccgtc gatccgcgtg agcaatggcg agatgagccc gaactcgggc cgcggtgcgt
     2401 actcgatccc aacgcggacc gggccagcct cgcatcggac cctccggacc aagacgccgg
     2461 gggcctcgga cccgatctcg tgctcaccgg cccactcgcc gagcgcacag gcgtcctcga
     2521 ggaccaggat gcccgacgcg gtccggaaga tcgtctcgag cacgagcgtc cccgcgatgt
     2581 agcgtctgga cacttcggcc tcatccacgg gcgcgatcga ccagtgcccc gcggcgtcgt
     2641 cgaggatcct ggagaacacg ggagcggcgt cgaaccgatc gaagcacagc cagtcgagag
     2701 acccgttggc cgagaccaac gccgaaccat ggcagtcggc gaggaagcca tgctcaccga
     2761 tcggcgtgcg gctcatcccg acctcatacc agacgcagga tggcgctcgg gcggaagatc
     2821 ggcccgacgg tgaccccgat cttcgcgcac ccggcgtccg ggaccccgat gtccgccagg
     2881 tagagctcgc cgacgacgtg gggggccgcc ccgagcccgg tcttgggaag cgccagcgtg
     2941 agcgtggcgg tggcccggac gcacggatca cccacccggc cggtgctggc ctccaggcca
     3001 ctcggaacat cgagcgcgca caccggcgcc ccctgctcgt tcgcccaccg gatcagccgg
     3061 gtggcgccac cgtgcggatc gcccctcagg gcatatccga gcagggcgtc gagcaccagg
     3121 tcggcttcga taggccggaa ggcgatcggg acgcccatct gctccagggt cctcagctgg
     3181 tgccgcggaa cctctgcgag ccggtcgggt ccctccgcca gcgtcacctg gacacgcacg
     3241 ccgcggttca ccaggtgccg agccgcgacg aggcctcccc cgccgttgcc tccccggccg
     3301 gcgaggacga ccgccgttcc gggaccgaac cgagcgagcg cgagctcggc cagcctggca
     3361 ccggcgttct ccatcatctg gatgaggagc agcccgagct gttccacggc cactcggtcg
     3421 acctcccgca tctgctctgc ggtcagggcc tggatcgagg acgacgccac ggtggcgatc
     3481 agggatccga ctcgatgcgc agccatccca cccctcagat cgcggtcgtt ccgcctcctc
     3541 ggtagaggac actcccgccg acggaggcat tcccgtcacg gagcctcctc ggcccttcgc
     3601 tgcggttcgg cccccgatct tggcagcggg tgctggcacc ctccccgcca cgtgggcttc
     3661 cacccgcgac ccttgggccc tgcacccttc cggatggggc cttgcgcggc cacaatccat
     3721 gtcatggcgg caccggtgag gacgcgcggg gttccggcgg agggaaccgg accgtcccca
     3781 ccgggcaggc ggtaccggta cgtcgcctgg gtcctctggc tgctggccgt cctggtgtac
     3841 ctgggggcca tggtggcagg agggttgacc gggtccatga gttcccaggg cgccgtggac
     3901 gtggtgctgg ggatgtcctc cgtcctcggg gcggtggcat acgccacggc cgggctgctc
     3961 atcgtgagcc ggcacccggg ccaagcggtc ggctggctgc tgatggcggt gcccctgtcg
     4021 ttcggagggg cgaacctcgt gggcggtctg gccacgtacg ggcttgcgac tcgccccggc
     4081 tccatcccct tcgccagggc cctggcctgg atcgaccggt ggagcctgct gccgatcctg
     4141 gggctgatcg ttctgctggt gctgctgttc cctgagggcc ggatcccctc ccggcggtgg
     4201 cgcccggtgt tgtggctcgc cgtgggtgcg ccgctggcgg cggcggcggg attcgcgctc
     4261 acccctggcc gcatgaccgg atccctggcc gacctgacct cggtggtggt tgccaacccg
     4321 ctgggtatcg acgcgctggc cgccccgatc ggggcgctca ccgtggcggc gtctctgggc
     4381 tcggtggcgg caggcctgct ggccgcgggt tccatggtgg tccgctaccg ctcgtcgaag
     4441 gcggaggtcc gacagcagat ccgctggatg gcgcttgtgg ccgcgatctt cctggtcgcc
     4501 ttcctgctgg agtacccggt cttcttcctc tccggcgggg ccgaccaggg tctcgggagc
     4561 gtcctatcca acgtcctgtt cgcggtcatg gcgctggcca tcatcctggg catcccggcg
     4621 gcatgcgcga tcgccatcct caagtaccgc ctctacgacc tggacgtcgt cgttcgaaag
     4681 accctggtgg tcggctccct ggccgtgttc ttcacggtgg tctacgcggg catcgtagcc
     4741 ggcctgggcg ccgtggtcgg gtcccgcttc gactcgacgg cctccttcct ggccgccgcc
     4801 gcggtggccg tggtcttcgc cccggcccga gaccgggccc gtcgcctggc cgaccggctc
     4861 gtctacggga ggcgggccac cccgtacgag atcctgaccg agttctcctc ccacatggcc
     4921 tccaccgagg cctccaccga cgtcctgtcc cggatggccg aggtcctggg gacgggcaca
     4981 ggtgccaggc gagcccgggt gtggctgcgg gtgggtcggg agcttagtcc ggcagccgcc
     5041 tggccctccg acgcgccggc cgtgccagcc gcgaccctgg ccgccgacgg gctccccgag
     5101 gtgcccgacc aggcggtctt cgaggtgcgc caccgtggcg agctgctggg cgccatcagc
     5161 gtgagcatgc ccgagtcgga tcccctcacc cccgccaagg agaagctggc ccgggacttc
     5221 gccgcgcagg ccagcctggc cctgcgcaac gtccggctga tcgaggagct acgggcctcc
     5281 cgtcagcggc tggtggccgc ccaggacgag gagcgccgcc gactcgagcg caacatccac
     5341 ggcggtgccc agcaacagct ggtcgcgctg gcggtgaagc tgggactggc cgagggtctg
     5401 atagaccagg atcccgtgga ggcccgggag atgatccagg ccctgcgagc agcctcgcag
     5461 gaggctttgg aggatcttcg cga
//
LOCUS       DASQQQ010000067         2492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_361690, whole genome shotgun sequence.
ACCESSION   DASQQQ010000067 DASQQQ010000000
VERSION     DASQQQ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361690"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /gene="purM"
                     /locus_tag="VE646_02925"
     CDS             <1..1022
                     /gene="purM"
                     /locus_tag="VE646_02925"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZD78982.1"
                     /translation="IASSYRAAGVDLEGANRLKRRLAALVDSTRGPEVLGELGGFGGC
                     YQIGELPEPDPVLVSSADGVGTKVLVAHWAGRHDTIGEDLVNHCVNDLLAAGARPLFF
                     LDYIAAGKLESDIVLALVAGLARACRANRAALIGGETAEMPDLYAAGHYDLAGFIVGV
                     APRARLGWRAEKGDVVLGLPSSGLHTNGYTLARRLVIDRGGHLPDEEVPWGGETWADI
                     LLAVHKSYLEAVEPLVDDPDLHAVAHVTGGGFAGNLPRVLPQGLAAQLERDAWTVPPL
                     FGYLAQAGEIDEEEMYRVFNMGIGLCLVVARDSAARVAECCAQAGEQAIEIGRVVEGE
                     GVEWR"
     gene            1013..1813
                     /locus_tag="VE646_02930"
     CDS             1013..1813
                     /locus_tag="VE646_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78983.1"
                     /translation="MALRAFLALALLLTLAAGARAQTISEARVREAIDDAAGGLGQAL
                     GGGSPLTGPASTTGGLLGLRTGLSLGLSAADIEDPRRPAGEIDVVLPVGSAHAAVGLT
                     RRVDVLGKIGLLAAGNDYEGIALTLGLGARVGLLRDAPALPDVSASLYRTWVDGLEFG
                     ELVDDEVAFDADVATWSLRLDASQGFGVVTPYAGVGLDRTGIDADYRIPSSQSSVGTE
                     IEGSIDTSDTHGKAYVGAELNLLVLRAAAEIGAGAAGTFLGVAVRLGP"
     gene            1831..>2492
                     /gene="ribD"
                     /locus_tag="VE646_02935"
     CDS             1831..>2492
                     /gene="ribD"
                     /locus_tag="VE646_02935"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HZD78984.1"
                     /translation="MVENRLAAFMERALELAERGGGATAPNPMVGCVLVAPDGSVIGE
                     GFHRRACGPHAEVEALEAARSCGHDPTGSLAVVTLEPCNAHGRTPPCTTALLEAGVGQ
                     IVFAVADPHTGKGGAERLRGEGVEAVGGVLEGPARRLIEPWLHFVETGRPLFHLKSAQ
                     TLNARVTRGRESVGWVTGPAARQAVHRLRRQHRAVMVGVGTVIADDPLLTVRDWPPAG
                     DG"
BASE COUNT          328 a          821 c          954 g          389 t
ORIGIN      
        1 cgatcgcctc cagctaccgc gcggcggggg tcgatctcga gggggcgaac cgcctcaagc
       61 gccggctggc cgcgcttgtc gactccacgc gcggtccgga ggtcttggga gagctcgggg
      121 gcttcggcgg atgctaccag atcggggagc ttcccgagcc cgatccggtc cttgtctcgt
      181 cagccgacgg cgtggggacg aaggtcctgg tggcacactg ggcgggccgg cacgacacga
      241 tcggcgaaga tctggtcaac cactgcgtga acgacctcct ggcggccggg gcccggccgc
      301 tcttctttct cgattacatc gccgccggca agctcgagtc cgatatcgtg ctggctctgg
      361 tggccggcct ggcccgcgcg tgccgcgcca accgcgccgc cctgatcggc ggcgaaacgg
      421 ccgagatgcc ggatctctat gccgccggcc actacgacct ggcggggttc atcgttgggg
      481 tggctccgcg ggcccgtctg ggctggcggg cggagaaggg cgacgtggtg ctggggcttc
      541 cctctagcgg cctccacaca aacggctaca cgctggcgcg ccggctggtg atcgatcggg
      601 gcggtcacct gcccgacgag gaggtcccat ggggcggcga gacgtgggcc gatatcctgc
      661 tggccgtcca caagtcctat ctggaggcgg tggagccgct cgtcgacgat cccgatctcc
      721 atgccgtcgc tcacgtgacc ggcggcggct tcgccggaaa tctcccgcgc gtcctgcccc
      781 aaggtctcgc ggcgcagctc gagcgcgatg cgtggaccgt gccgcccctg ttcgggtatc
      841 tggcccaggc tggggagatc gatgaggagg agatgtaccg ggtcttcaac atggggatcg
      901 gcctgtgcct ggtggtcgcc agagattcgg ccgcgcgggt cgcggagtgc tgcgcccagg
      961 cgggcgagca ggcgatcgag atcggccggg tggtagaggg agagggcgtc gagtggcgct
     1021 gagagcgttt cttgcgctgg ccttgcttct gacgctggcg gcgggcgccc gcgcccagac
     1081 catcagtgag gcccgggtcc gcgaggcgat cgacgacgcc gcgggaggtc tgggccaggc
     1141 cctcggcggc gggagcccct tgaccggtcc cgcctccacc accggtgggc tgctcggtct
     1201 cagaacgggt ctctcgctgg ggctgagcgc agccgacatc gaggaccccc gtcgtccggc
     1261 aggggagatc gacgtggtgc tgcccgtggg aagcgcgcac gccgccgtgg gactcactcg
     1321 ccgggtcgac gtcctgggga agatcgggct cttggccgcc gggaacgact acgagggaat
     1381 cgccctcacg ctagggcttg gagcccgggt ggggcttctc agggacgccc cggccctgcc
     1441 cgacgtgagc gcatcgctct accgtacctg ggtcgacggc ctcgagttcg gcgagctggt
     1501 ggacgacgag gtcgccttcg atgccgatgt cgccacctgg tcgctgcgcc tggacgcgtc
     1561 gcagggcttc ggcgtcgtga ccccttatgc cggggtcggg ctggaccgga cgggcatcga
     1621 cgcggattac cggatcccat ccagccagtc gagcgtcggg accgagatcg agggctcgat
     1681 cgataccagc gatacccacg gaaaggctta cgtcggggcc gagctgaacc tgctggtgct
     1741 gcgcgcggcg gctgagatcg gcgctggggc cgccgggacg tttctcggcg tggccgtccg
     1801 gctcgggccg tagccgtgtc ccgggatcag gtggtagaga accggctcgc agccttcatg
     1861 gagcgggccc tcgaactggc cgagcgcggt ggcggcgcca ccgcccccaa cccgatggtg
     1921 ggctgcgtcc tggtcgctcc cgacggatcg gtgatcgggg agggctttca ccgacgggcc
     1981 tgtggccccc acgccgaggt cgaggccctg gaggcggccc ggagctgcgg acacgatccc
     2041 accggctcgc tggccgtcgt gactttggag ccgtgcaacg cccatggccg gacgccgccc
     2101 tgcacgacgg ccctgctcga ggccggggtg gggcagatcg tcttcgcggt ggccgatccc
     2161 cataccggga aggggggagc cgagcggcta cgaggcgagg gggtcgaggc ggtcggcggc
     2221 gttctcgagg gcccggctcg gcgattgatc gaaccttggc ttcacttcgt cgagaccggc
     2281 cggccactgt ttcatctgaa gagcgcgcag acgctgaacg cccgggtgac ccgcggccgc
     2341 gagagcgtgg ggtgggtgac cggacccgcc gcgcgacagg ccgttcaccg gctccgccgc
     2401 cagcatcggg cggtgatggt gggggtgggg acggtgatcg ctgacgatcc gcttctcacg
     2461 gtgcgggact ggccccccgc gggggatggc ga
//
LOCUS       DASQQQ010000068        11209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_60724, whole genome shotgun sequence.
ACCESSION   DASQQQ010000068 DASQQQ010000000
VERSION     DASQQQ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11209
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60724"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VE646_02940"
     CDS             complement(<1..895)
                     /locus_tag="VE646_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD78985.1"
                     /translation="MSSRPLLVVEDLRVWYGTERGAVRAVDGVSFTISAGETLGLVGE
                     SGCGKSTLGRGIMGLLPDSAAVAGRVGFDGVDVLGLSPKDRRALHGPEIGMIFQEPMT
                     RLNPLMRISDHFRETLKTHEPGTSDQEMRRRSLEALGGMGIPPTRFRQYPHEFSGGMR
                     QRIMIALALVLRPKLVICDEPTTALDVLVEAQILSILGDLKRNFDTALLFITHNLGIV
                     AETCDRIAVMYAGKMVEEGGTQATFRTPAHPYTRELLGSIISLQTTGLRSIPGAPPDL
                     SDPPPACRFHPRCPDAMRVCAV"
     gene            complement(892..1950)
                     /locus_tag="VE646_02945"
     CDS             complement(892..1950)
                     /locus_tag="VE646_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD78986.1"
                     /translation="MQREAAKREREAVPPTPTPVRRRGALARMLAPVRAARGMPRWIL
                     WTGAGISGSFLVLAIFASWIAPYGFDQYRTAEGVRFVKQQAPSAAHWFGTNVMSTDVL
                     SRVVFGSRTALEVVVLALAFALSLGLPLGLISGYFGGAADRVLVLVMDALFAFPYLLL
                     AIVIAFLLSNSIGRGVMSAAIAIAVVYTPQYFRVVRNHVISVREEPYVEAARALGARP
                     RTVIRKYVLQNVIQNVPVIATLNAADAILTLAALGFLGYGIQPTEAAEWGYDIQRALA
                     DAGAGIWWTGLFPGLAIVLLVTGLTLLGEGLNDVVNPIIRRRSFRKVRFPEEHVEPSE
                     APPSWAEIDPASPPREAP"
     gene            complement(1950..2978)
                     /locus_tag="VE646_02950"
     CDS             complement(1950..2978)
                     /locus_tag="VE646_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD78987.1"
                     /translation="MARETGSLHRYLLTRLALAVPMVLILLTLVFTLMRVAPGDPITA
                     ALGGRLPAAELQRRRAAAGFDRPVIVQYGEYLWSVAHLDFGTTLIDNRPVLSVIAQNG
                     SATLELTAWAMLVAVGVGVPLGLLAGRFRDTGTDLGFRLFGIVIYAAPVFFLGFLAQL
                     VFGKYLNWLPTSGRASPLIGYELQTHTQLFVIDAIIDRNWSALTDVLRHLVLPAMTLG
                     LVIGGVFIRMVRVNVMQTLKGDYVEAARARGVKERWVLFRHAFKNALVPVITVMGLQF
                     ALLLSGAVLTEETFNWPGLGFQLVRYLNNRDYIAVQGIITFAGLVVVLVSLAIDVLTA
                     WVDPRVRY"
     gene            complement(3051..4673)
                     /locus_tag="VE646_02955"
     CDS             complement(3051..4673)
                     /locus_tag="VE646_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZD78988.1"
                     /translation="MRARILHGRVLAFLAVVGLLAAACGGGGGGGEEPTGSASGGGNA
                     QATIVHGTTDTVVSLDPAGSYDFGSWQIIYNTMDGLLEIPPQGNTPEPALADSCDFTD
                     PTTYQCTLKSGLKFSDGSPVTSEDVVYSLQRNIEIADPNGACSLLAALADCKKWDPKA
                     VDTPDPQTVVFHLRYPDGTWPFILTTPAAFVVPKASYPEKDKQPDEPGKVIGTGRYVL
                     KDYRPGEQAVLEANPDFWGDAPANARVIVQYFSNESALKLALEQQEVDIAWRSLGPTA
                     VSALENESGIQVLKGPGTEIRYLVFNLKFSPGKQLAVRKAVAYTIDRAAIAKNAWNGT
                     VEPLYSMVPTEFAGHTDAFAEMYGENPDVNAAKKVLQDAGISTPVDLEIWYTPTHYGE
                     ATADEYAEIKRELEGSGLFKVTLKSAEWDQYSNAAFTDQYPIYQLGWFPDYVDADDYT
                     APFYSKGSFLNDHYSSPKMESLLKQEKAETDPAKREQIFAQIQKLGAEDVPIIPIWQG
                     FQLAAIREGITGVDKTLDASFQFRYWLVSKAS"
     gene            complement(4742..5575)
                     /locus_tag="VE646_02960"
     CDS             complement(4742..5575)
                     /locus_tag="VE646_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HZD78989.1"
                     /translation="MAEVKGFLPPRSPQGRASIVSAPPWHYSGDLLTVEYRAAPGAVA
                     RWLPDPIEPADEDPDAVALIFADWQSCSDSFEELVDPVRSQYKECFMVVRCRYRGERY
                     SRCINIWVDKDFALARGWHQGYPKKLGSIWMTRPVRYGKAGPRLEPGGRFGASVSAND
                     RRIVEARFTITGPSQSNGFVNALPMLHDRYAPSIVEGEPPSMDELVTMKSFDWEGSEI
                     WEGDAELSLFDSPTEELTSLTPTEAIGAYYRSTGVTWGEGTKLADRRSRPGHPARRPR
                     P"
     gene            5625..6470
                     /locus_tag="VE646_02965"
     CDS             5625..6470
                     /locus_tag="VE646_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014058939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZD78990.1"
                     /translation="METRRILIDGQATAVIREGDDLVASDGRRVAAGSALHLPPCEPT
                     KIICIHLNHESRVKEFLATLPPAPTYFHKPTSSLNAHGGDVVRPPRCRYLNYEGEIAI
                     VIGRECRHVSRQDAPGYIAGYTIANDFGLHDFRDTDAGSMLRVKGSDTLCPLGPGLVD
                     DWDFRGKRIRTLVNGEVRQDGTTDEMVWDMPYLVADLARTITLLPGDVILSGTPANSR
                     PVEPGDLVEVEVEGLGVLRNRVVEGTVPIRDDVGAQPSASEEVVSTALGGDWEFRGIR
                     PPSKP"
     gene            complement(6478..6612)
                     /locus_tag="VE646_02970"
     CDS             complement(6478..6612)
                     /locus_tag="VE646_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78991.1"
                     /translation="MKRFLPFALIGLIAILGLAFGMRAMRGRRGAEEPFEEMVSEGVS
                     "
     gene            6754..8058
                     /locus_tag="VE646_02975"
     CDS             6754..8058
                     /locus_tag="VE646_02975"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HZD78992.1"
                     /translation="METKGKRLRLLYPDIHGLERGKYLLGDWTGTAAFCIGVYPLTFD
                     KEILPIPNTQFDIGLPDIEVHLDRDSLRPGWEEGTVIGLGDAEFRGRPTPLDPRHVLR
                     QAMRWWQERGLTPQLAYEFEFYLLEPDPDTGAWRSVPMPSHRVYGTGPAIDPTGVVDE
                     IVKTALACGFEVESWGSEYDTAQYEVNIKYRDCMAATDDGFLFRLLAHEVASRRGYWP
                     TFIGRPFNDRGGSGLHVNLSFRREDGSNAFHDPASADGLSELARRCLAGMLAHHEGMA
                     ALLAPHVNAYKRLQPDMLNGYWANWGYDDRTVTVRVPPGRGETSRLEHRTADGAANPY
                     LAGATILNAARLGVEHELEPPPPQEPGADPNSEVRIPPSLDRSLLVLEADKELCELMG
                     PELIEAFGILKRAEWERYLKNVENPSTLDVTAWELDYYVPFF"
     gene            8239..9456
                     /locus_tag="VE646_02980"
     CDS             8239..9456
                     /locus_tag="VE646_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003987887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HZD78993.1"
                     /translation="MKVLLVGVGGVGEAVAIVAKDRPWLERMVLADFNVDRANEVQAR
                     LGDPQRFPVEFVDAGEKETVVELARRHGADLILNAVEPVFDMPIFGAALEAGATYMDM
                     AMSLSRRHTSEPYRLPGVKLGDEQFQAATEWEDRGLLALVGMGVEPGMADVFAKYAAK
                     HLFDEIDEIGVRDGANLEVEGYGFAPNFSIWTAIEECLNPPILWDRERGWFTTETFSE
                     PEVFEFPEGIGAIECVNVEHEEVLLIPRWIDAGRVTFKYGLGNEFINVLKVIQLLGLD
                     STEPVKVKGAEVAPRDVLVALLPDPAHLGDKMTGKTCAGTWVKGVKEGNPREVYLYQV
                     ADNEACMRDYGVQAVVWQTAVNPVIAMELLETGAWKGTGVLGPEAFDPDPFMELMPTY
                     HFPWGLREMTPTG"
     gene            9569..10822
                     /locus_tag="VE646_02985"
     CDS             9569..10822
                     /locus_tag="VE646_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007379203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZD78994.1"
                     /translation="MAVTEIDLVNPDNYVPGVPFEWFDRLRREQPVSWQDEPGPNQGF
                     WAVTRYKDLVAVHMDWHTYSSEVGAVALEELDPEQLQIRKTMLETDPPRHRELRNICN
                     KRFSGRAVGRYENFIREVARTVLDRALEKKEFDFVPEISRELPIRFLCAIFTVPQEDA
                     PTLIGWGDAMIGNQDPEFSAAVVDQVDTEEFKYLPFRSPIAAKVFEYADRQRDDRLEE
                     PRDDVITALTVAQSEGVLSEREFENYFSLLLIAGNETTRHTISHGMKALMEHPDQLRA
                     LKDDPSKIPVAVEEILRWATPVMHFRRTATKDTELAGQRIREGDKVVTWYVSANRDEE
                     VFADPHRFDIERRPNEHVTFGPGGPHFCLGAHLARLETKVLFQELLPRLRSIEPTGPV
                     ERMRSNFVNGIKHMPVRVELAGPGG"
     gene            complement(10829..>11209)
                     /locus_tag="VE646_02990"
     CDS             complement(10829..>11209)
                     /locus_tag="VE646_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD78995.1"
                     /translation="ALLFGRLFDRAGIGILVPLTIVGALFAPLVFLGGPWAGLAGASL
                     WGLGMGVHESIVPAAVAPMVPANRRASAYGIFTGAYGVAWFLGSVVMGALYAFSLGAV
                     VAFSVVAELAAIPFILRVRHHQTR"
BASE COUNT         1818 a         3908 c         3849 g         1634 t
ORIGIN      
        1 gcaccgcgca cacgcgcatg gcgtcggggc atcggggatg gaaccggcag gcgggcgggg
       61 ggtcgctgag gtccgggggc gcaccgggga tggaccgcag ccccgtggtc tgcagcgaga
      121 tgatcgagcc aagcagctcc cgcgtgtagg ggtgggccgg ggtgcggaac gtggcctgcg
      181 tgccgccctc ctccaccatc ttgcccgcat acatcacggc gatccggtca caggtctccg
      241 ccacgatccc caggttgtgg gtgatgaaca gcaaggccgt gtcgaagttg cgtttgaggt
      301 cccccaggat gctcaggatc tgcgcctcca ccagcacgtc cagggcggtg gtcggctcgt
      361 cgcagatcac gagcttgggc cggagcacca acgccagcgc gatcatgatc cgctggcgca
      421 tgccgccgga gaactcgtgc gggtactgac ggaaccgcgt gggcgggatg cccatgccgc
      481 ccagggcctc cagggaccgg cggcgcatct cctggtccga ggtccccggc tcgtgggtct
      541 tgagcgtctc ccggaagtga tcggagattc gcatcagcgg gttcagccgg gtcatgggct
      601 cctggaagat catgccgatc tccgggccgt ggagcgcccg tcggtccttg ggcgacaggc
      661 ccaggacgtc cacgccgtcg aacccgacgc gtccggccac ggccgcgctg tcggggagga
      721 gccccatgat cccccgcccc agggtggact tcccgcaccc ggactccccc accagcccga
      781 gcgtctcgcc ggccgagatc gtgaacgaca cgccgtccac ggcccgcacg gcgccacgct
      841 cggtcccgta ccagacccgg aggtcctcca cgaccagcag gggcctggag ctcatggagc
      901 ctcccggggc ggggacgccg ggtcgatctc ggcccagctc ggaggggcct cggagggctc
      961 cacatgctcc tcggggaacc ggaccttacg gaaggagcgc cggcggatga tcgggttcac
     1021 cacgtcgttc aggccctcgc ccaggagggt cagcccagtc accagcagga cgatggccag
     1081 gccagggaac agcccggtcc accagatgcc ggcgccggca tcggccagcg cccgctggat
     1141 gtcgtagccc cactcggccg cctcggtggg ctggatcccg tacccgagga agcccaacgc
     1201 cgccagggtg aggatggcgt cggccgcgtt gagcgtggcg atcaccggga cgttctggat
     1261 cacgttctgc agcacgtact tgcggatcac ggtccggggc ctggcgccca gcgcccgcgc
     1321 cgcctccacg tagggttcct cccgaacgct gatcacgtgg ttgcgcacca cccggaagta
     1381 ctgcggcgtg tacacgacgg cgatcgcgat ggcggcgctc atcacgcccc ggccgatcga
     1441 gttcgacagc aggaacgcga tcacgatcgc cagcagcagg taggggaacg cgaacaaggc
     1501 atccatcacc agcaccagga cccggtcggc ggcgccgccg aagtacccgg agatcagacc
     1561 caggggcaat cccagggaca gcgcgaaggc cagcgccagc accaccacct ccagtgccgt
     1621 gcgcgagccg aacacgaccc gcgatagcac gtcggtggac atcacgttgg tgccgaacca
     1681 gtgggcggcc gacggcgcct gctgcttcac gaaccggacg ccctcggccg tgcggtactg
     1741 gtcgaacccg tagggcgcga tccagctcgc gaagatggcc aggacgagga aggagccgct
     1801 gatccccgcc ccggtccaca ggatccagcg gggcatgccg cgggccgccc gcaccggggc
     1861 gagcatccgc gcgagggctc cccgccgccg gacgggggtg ggggtcgggg ggaccgcttc
     1921 tcgttcccgc ttggccgctt cccgctgcat cagtaccgca ccctcggatc cacccacgcc
     1981 gtcagcacgt cgatcgccag gctgaccagc accacgacca agccggcgaa cgtgatgatg
     2041 ccctgtaccg cgatgtaatc acgattgttc aggtaccgga ccagctggaa gccgagcccg
     2101 ggccagttga aggtctcctc ggtcagcacc gcccccgaca gcagcagcgc gaactgcagc
     2161 cccatcaccg tgatcacggg caccagggcg ttcttgaacg cgtggcggaa cagcacccag
     2221 cgctccttca ccccccgggc gcgagccgcc tccacgtagt cgcccttcag cgtctgcatc
     2281 acgttcaccc ggaccatcct gatgaacacc ccgccgatca ccagccccag ggtcatcgcg
     2341 gggaggacca ggtgccgcag gacatccgtc agggccgacc agttccgatc gatgatcgcg
     2401 tcgatcacga acagctgcgt atgcgtctgg agctcgtacc cgatcagcgg gctggcccgt
     2461 cccgaggtgg gcaaccagtt caggtacttg ccgaagacca gctgggccag gaagcccagg
     2521 aagaagaccg gagccgcgta gatcacgatc ccgaacagcc ggaagcccag gtccgtcccc
     2581 gtatcgcgga accggcccgc cagcaggccc agcgggaccc ccacgcccac cgccacgagc
     2641 atggcccagg cggtgagctc cagcgtggcc gagccgttct gcgcgatcac cgagagcacc
     2701 ggccggttgt cgatcagcgt ggtgccgaag tccaggtggg ccacgctcca caggtactcc
     2761 ccgtactgga cgatgaccgg ccggtcgaag ccggcggccg cccgccgccg ctgcagttcg
     2821 gccgccggga gccgtcctcc cagagccgcg gtgatcgggt cgcccggggc cacacgcatc
     2881 agggtgaaga ccagcgtgag caggatcagg accatgggca cggccagggc caggcgggtg
     2941 agcaggtacc gatgaaggga gcccgtttcc ctggccatgt cgttacctcc gggcggcgga
     3001 cagcggacgg gggcccggtg cgatcaccga gcccccgtcc gtcgctccga tcacgaggcc
     3061 ttggatacca gccagtaccg gaactggaac gaggcgtcca gcgtcttgtc cacgccggtg
     3121 atcccctcgc ggatcgccgc cagctggaag ccctgccaga tcgggatgat cggcacgtcc
     3181 tccgcgccca gcttctggat ctgcgcgaag atctgctccc gcttggccgg gtccgtctcg
     3241 gccttctcct gcttcaggag gctctccatc ttcgggctgc tgtagtggtc gttcaggaag
     3301 gatcccttcg agtagaaggg ggccgtgtag tcgtcggcat cgacgtagtc cgggaaccac
     3361 cccagctggt agatcgggta ctggtccgtg aaggcggcgt tcgagtactg gtcccactcg
     3421 gccgacttca gcgtcacctt gaacaggccg ctgccctcga gctcacgctt gatctccgcg
     3481 tactcatcgg cagtggcttc cccgtaatgg gtaggcgtgt accagatctc caggtccacg
     3541 ggcgtgctga tgccggcgtc ctgcagcacc ttcttggccg cgttgacgtc ggggttctca
     3601 ccgtacatct cggcgaacgc gtccgtgtgc ccggcgaact cggtgggcac catcgagtac
     3661 aggggctcca ccgtgccgtt ccaggcgttc ttggcgatgg ccgcccggtc gatcgtgtag
     3721 gcgacggcct tgcgcaccgc cagctgcttg ccgggcgaga acttgaggtt gaacaccagg
     3781 taccggatct cggtgccggg ccccttgagg acctggatcc cggactcgtt ctccagggcc
     3841 gagaccgcgg tcgggccgag gctccgccag gcaatgtcca cctcctgctg ctccagcgcc
     3901 agcttcaggg cggactcgtt cgagaagtac tgcacgatca cccgggcgtt ggccggggcg
     3961 tcgccccaga agtcggggtt ggcctcgagc accgcctgct caccgggacg gtagtccttc
     4021 aggacgtacc gtccggtccc gatcaccttg ccgggctcgt cgggctgctt gtccttctcc
     4081 gggtaggagg ccttgggcac cacgaaggcg gccggcgtgg tgaggatgaa cggccacgtg
     4141 ccgtcggggt accgcaggtg gaacaccacg gtctgcggat ccggcgtgtc gacggccttc
     4201 gggtcccact tcttgcagtc ggccagcgca gccagcaggc tgcaggcccc gttcgggtcc
     4261 gcgatctcga tgttgcgctg gagcgagtag accacgtctt ccgacgtcac cggcgatccg
     4321 tcgctgaact tcagtcccga cttcagggtg cactggtacg tggtggggtc cgtgaagtcg
     4381 caggaatcgg ccagcgccgg ctccggggtg ttgccctgcg gcgggatctc cagcaggccg
     4441 tccatcgtgt tgtagatgat ctgccaggaa ccgaagtcgt acgagccggc cgggtcgagc
     4501 gagaccaccg tgtccgtggt gccgtggacg atcgtggcct gggcgttgcc gccgccgctc
     4561 gcgctgcccg tcggctcctc gcccccgcct cctccgccgc cgcaggccgc cgccaacagg
     4621 cccaccacgg cgaggaacgc gaggacccgg ccatgcaaga tccttgccct catcgagcca
     4681 ccccctcctc gtccggcgat ccggacgccc ccgggcgttc gccgccgtat cctatctctc
     4741 cttacggtcg gggacggcgg gccggatggc cggggcggga tcggcggtcc gccagcttgg
     4801 tgccctcgcc ccaggtcacc ccggtgcttc ggtagtaggc cccgatcgcc tcggtgggcg
     4861 tcaggctggt gagctcctcg gtgggcgagt cgaacagcga gagctcggcg tcaccctccc
     4921 agatctccga gccctcccag tcgaagctct tcatcgtcac gagttcgtcc atcgacggcg
     4981 gttcgccttc gacgatcgag ggggcgtacc ggtcgtgcag catgggcagg gcgttcacga
     5041 agccgttgct ctgggaggga ccggtgatcg tgaagcgcgc ctccacgatc cgtcgatcgt
     5101 tcgcgctcac ggacgcgccg aacctccctc ccggctccag cctgggaccg gccttcccgt
     5161 accgcaccgg ccgggtcatc cagatcgagc ccagcttctt ggggtagccc tggtgccaac
     5221 cccgggccag ggcgaagtcc ttgtcgaccc agatgttgat gcagcgcgag taccgctccc
     5281 cccggtaccg gcagcgcacc accatgaagc actccttgta ctgggaccgc accggatcga
     5341 ccagctcctc gaagctgtcg ctgcacgact gccaatccgc gaagatcagg gccacggcgt
     5401 cggggtcctc gtcggcgggc tcgatggggt cgggcagcca gcgggccacc gccccgggcg
     5461 cggcccggta ctccaccgtc agcaggtcgc ccgagtagtg ccagggggga gcggagacga
     5521 tggaggctcg cccctggggc gagcgcgggg gcaggaagcc cttcacctcc gccatgcgcc
     5581 gcatactacc cttccggttg cacgcggccg gagggagacg ccggatggag acgcgaagga
     5641 tcctgatcga cgggcaggcg accgccgtga tccgcgaggg cgacgatctg gtggcatcgg
     5701 acggtcggcg ggtggccgcc ggctcggccc tccacctgcc cccctgcgag cccacgaaga
     5761 tcatctgcat ccatctgaac cacgagagcc gggtgaagga gttcctggcc acgctgcccc
     5821 cggcgccgac gtacttccac aagcccacca gctcgctgaa cgcgcacggc ggcgacgtgg
     5881 tccgtccgcc ccgatgccgg tacctgaact acgagggcga gatcgcgatc gtgatcggcc
     5941 gggagtgccg gcacgtctcg cgccaggacg cccccgggta catcgccggg tacacgatcg
     6001 ccaacgactt cggactgcac gacttccgag acacggacgc cggctcgatg ctacgggtga
     6061 agggatcgga caccctctgt ccgctcggcc ctgggctggt ggacgactgg gacttccgcg
     6121 ggaagcggat ccggaccctg gtgaacggcg aggtgcggca ggacggcacc accgacgaga
     6181 tggtgtggga catgccctac ctggtggcgg acctggcccg gaccatcacg ctgctgcccg
     6241 gggacgtgat cctgtcgggg accccggcca actccaggcc ggtcgagccc ggggacctgg
     6301 tggaggtaga ggtggagggg ctgggcgtcc tccgcaaccg cgtcgtggag ggcacggtcc
     6361 cgatccggga cgacgtgggc gcccagccct cggcctccga ggaggtcgtc tcgacggccc
     6421 tcggcgggga ttgggagttc cgagggatcc gcccgccgag caagccttag ccgaggatca
     6481 ggacacgccc tccgagacca tctcctcgaa cggctcctcg gccccccgac ggccccgcat
     6541 ggctcgcatc ccgaaggcca gccccaggat cgcgatgagc ccgatcagcg cgaacggcag
     6601 gaacctcttc atggtcgcct cccctcggtt cccggcacgc ccagcctacc cccttgcgcc
     6661 gatccggcgc gaccgaggct cgttcgcgac cgaacgaggg gtgggctatc ctgcggcggt
     6721 cgcccgccga gcccagaaca caggagcgga ccgatggaaa cgaagggcaa gcgtcttcgc
     6781 ctcctctatc ccgacatcca cggcctggag cgtgggaagt acctcctcgg cgactggacc
     6841 ggcaccgccg ccttctgcat cggcgtgtac ccgctgacct tcgacaagga gatcctgccc
     6901 atccccaaca cgcagttcga catcgggctc cccgacatcg aagtgcacct ggaccgcgac
     6961 tcgctgcgac ccggctggga ggagggcacg gtgatcggcc taggggacgc cgagttccga
     7021 ggacgaccca cgccgctcga tccccgccac gtcctgcgcc aggcgatgcg gtggtggcag
     7081 gaacggggac tcaccccgca gctggcctac gagttcgagt tctacctgct ggagcccgac
     7141 ccggacacgg gcgcgtggcg ctcggtcccg atgccctcgc atcgcgtgta cggcaccggg
     7201 ccggcgatcg accctaccgg cgtggtggac gagatcgtca agaccgcact ggcatgcggc
     7261 ttcgaggtcg agagctgggg ctccgagtac gacacggcgc agtacgaggt caacatcaag
     7321 taccgggact gcatggcggc cacggacgac ggcttcctgt tccgtctgct ggcgcacgag
     7381 gtcgcctcgc gtcgcggcta ctggcccacc ttcatcgggc gacccttcaa cgaccgaggt
     7441 ggcagcggcc tgcacgtgaa cctcagcttc cgcagggagg acggctccaa cgcgttccac
     7501 gacccggcct cggccgacgg cttgtcggag ctcgcccggc gctgcctggc ggggatgctc
     7561 gcgcaccacg agggcatggc ggccctgctg gccccgcacg tgaacgcgta caagcggctg
     7621 cagccggaca tgctgaacgg ctactgggcg aactggggct acgacgaccg caccgtcacg
     7681 gtgcgggtcc cgccggggcg cggcgagacc agccggctgg agcaccggac ggcggacgga
     7741 gccgccaacc cgtacctggc cggggccacc atcctgaacg cggctcgcct gggcgtggag
     7801 cacgagctcg agccgccgcc gccccaggag ccaggcgccg atccgaacag cgaggtcagg
     7861 atcccgccca gcctggaccg ttccctgctg gtgttggagg ccgacaagga gctgtgcgag
     7921 ctcatggggc cggagctgat cgaggccttc ggcatcctga agcgggccga gtgggagcga
     7981 tacctcaaga acgtggagaa tccgagcacc ctggacgtca ccgcctggga gctcgactac
     8041 tacgtgccgt tcttctaggc gcgccgcccg ggttcggccc ccgccgcgcc ggccgagcgc
     8101 ggttccgccc aggcgtccaa cccccgaggt cctcctgctc cccgctggag ggtgcgggcg
     8161 ccgggcgctc tgccaagatg gtccgggacg ctccccgagc cggtcggctc tcgggcgcag
     8221 gagcgaggag ggcgtgacat gaaggtcctg ttggtcgggg tcggaggcgt cggcgaggcc
     8281 gtggcgatcg tggccaagga ccgtccctgg ctggaacgga tggtcctcgc ggacttcaac
     8341 gtcgaccgag cgaacgaggt gcaagccagg ctgggcgacc cccagcggtt ccccgtggag
     8401 ttcgtcgacg ccggcgagaa ggagaccgtc gtcgagttgg cccggaggca cggcgccgat
     8461 ctgatcctga acgccgtgga gccggtgttc gacatgccga tcttcggcgc cgccttggag
     8521 gccggcgcca cctacatgga catggcgatg tccctttccc gccgtcatac ctccgagccc
     8581 taccggctgc ccggggtgaa gctcggcgac gagcagttcc aggcggccac cgagtgggag
     8641 gatcggggcc tcctggcgct ggtcggcatg ggcgtggagc cggggatggc ggacgtgttc
     8701 gcgaagtacg cggccaagca cctgttcgat gagatcgacg agatcggcgt ccgggacggc
     8761 gccaacctgg aggtcgaggg ctacgggttc gctccgaact tctcgatctg gaccgcgatc
     8821 gaggagtgcc tgaacccgcc gatcctgtgg gatcgcgagc ggggatggtt caccaccgag
     8881 accttctccg aaccggaggt cttcgagttc ccggagggca taggggcgat cgagtgcgtg
     8941 aacgtggagc acgaggaggt cctgttgatc ccccgctgga tcgacgccgg ccgcgtcacg
     9001 ttcaagtacg gcctggggaa cgagttcatc aacgtgctca aggtgatcca gctgctcggt
     9061 ctggactcga ccgagcccgt gaaggtgaag ggcgcggagg tggcgccacg cgacgtgctg
     9121 gtggcactcc tgcccgaccc cgcccacctg ggcgacaaga tgaccggcaa gacctgcgcg
     9181 ggcacctggg tcaagggcgt gaaggagggc aacccgcgcg aggtgtacct gtaccaggtg
     9241 gccgacaacg aggcctgcat gcgcgactac ggggtccagg ccgtcgtgtg gcagaccgcc
     9301 gtgaacccgg tgatcgcgat ggagctgctg gagaccgggg cctggaaggg caccggcgtc
     9361 ctcgggcccg aggccttcga ccccgatccc ttcatggagc tgatgcccac ctaccacttc
     9421 ccctggggcc tgagggagat gacgcccacc gggtagcccg ccgccgcgcc gcggtggggt
     9481 cacccttgcg acccgtgcca gcgccttgcc cggcgaggtc gttcggtagc aaaatatatg
     9541 aacgtccgat tcctggtggg ggaactccgt ggccgtgacc gagatcgacc tcgtcaaccc
     9601 cgacaactac gtccccggcg tcccgttcga gtggttcgat cggctccgcc gggagcagcc
     9661 cgtgtcctgg caggacgagc ccggccccaa ccaggggttc tgggcggtca cccgatacaa
     9721 ggacctcgtg gccgtccaca tggactggca cacgtactcg tccgaggtcg gcgcggtggc
     9781 cctggaggag ctcgaccccg agcagctgca aatccgcaag acgatgctcg agacggaccc
     9841 gcctcgccac cgcgagctca ggaacatctg caacaagcgg ttctcgggcc gggccgtggg
     9901 acggtacgag aacttcatcc gtgaggtggc gcgcacggtc ctggatcggg ccctggagaa
     9961 gaaggagttc gacttcgtcc cagagatctc ccgagagctc ccgatccggt tcctgtgtgc
    10021 gatcttcacg gtcccccagg aggacgcccc gacgctgatc ggatggggcg acgcgatgat
    10081 cgggaaccag gatccggagt tctccgccgc ggtggtggat caggtcgaca cggaggagtt
    10141 caagtacctg cccttccgca gcccgatcgc ggccaaggtg ttcgagtacg cggaccgaca
    10201 gcgggacgac cgtctggagg agccccggga cgacgtgatc acggccctca ccgtggccca
    10261 gtccgagggc gtgctgtccg agcgcgagtt cgagaactac ttctccctgc tcttgatcgc
    10321 ggggaacgag accacccgcc acacgatcag ccacgggatg aaggcgctga tggagcatcc
    10381 cgaccagctg cgggccctga aggacgatcc ctcgaagatc ccggtggcgg tggaggagat
    10441 cctccgctgg gccaccccgg tgatgcactt ccgccgcacc gcgacgaagg acaccgagct
    10501 cgcgggacag cggatccggg agggcgacaa ggtcgtgacc tggtacgtca gcgcgaaccg
    10561 cgacgaggag gtcttcgcgg acccccaccg cttcgacatc gagcggcgtc cgaacgagca
    10621 cgtcaccttc ggccccgggg gcccgcactt ctgcctgggg gcgcacctgg cccggctgga
    10681 gaccaaggtg ctgttccagg agctgctccc ccggctgagg tcgatcgagc ccaccggccc
    10741 ggtggagcgg atgcgctcga acttcgtcaa cgggatcaag cacatgccgg tgcgtgtgga
    10801 gctggccgga ccaggggggt gaacggctct acctcgtctg gtggtgtcgg acgcgcagga
    10861 tgaaggggat cgcggcgagc tcggccacca ccgagaacgc caccacggcg cccagcgaga
    10921 aggcgtacag ggcccccatc acaacgctcc ccaggaacca cgccaccccg taggccccgg
    10981 tgaagatccc gtaggcggag gcccgtcggt tggccggcac catcggcgcc acggcggcgg
    11041 gcacgatcga ctcgtgcacg cccatcccga gcccccatag cgacgccccg gccaggccgg
    11101 cccaaggccc acccaggaat accagggggg cgaacagggc cccgacgatc gtcagcggca
    11161 ccaggatccc gatgcccgcc cgatcgaaca gccggccgaa gagcagggc
//
LOCUS       DASQQQ010000069         4522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_170401, whole genome shotgun sequence.
ACCESSION   DASQQQ010000069 DASQQQ010000000
VERSION     DASQQQ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170401"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            913..4497
                     /locus_tag="VE646_02995"
     CDS             913..4497
                     /locus_tag="VE646_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD78996.1"
                     /translation="MPKARAIRSIATAEYGVARPAGVTYVPDAGTLLVAGEVLLELTP
                     ADDLVATHSLPARFDAATLAFDPAFGRLTSVTDGQLLTGRPQGLSDVRTLDLRRLQLG
                     DPAGTTFDPESGDWFILDLRGPSLVRVRSQRDLSTAPVSRIPMRPLGSGGLRGLAYNP
                     ADGLLYTAGQGGDGDLLYAIDDSGTVRGTYDLSETGIRDLRAMVFAPSADPTDDPSTV
                     HLYVADAGGSATYGSVTEVTLEAAVTAAAATDVATLVQRIDTSQFTPASPDPSGVVYL
                     PAERRLAITDSEVDETTGAGYHGVNLWQVTSTGSVQDTGTTVPFSIEPTGLGYDPASR
                     TLFISDDDKNRVFLDRPGPDGRFGTSDDDVSWIDVGAYSTDAEDPEFDPATGHLFFID
                     GVAREIFEIDPVNGVFGDSDDQVSSFDLARYGANDLEGLGSDPARNTLLAGERSAKLI
                     YELTKDGALVRTIDASGISGMGHISGLAMAPASYDASRMSYWIVDRGTDNGSNPNEND
                     GQLFEISASTSVNAPPAVDAGSDQTVVQPDRATLAGVVSDDGLPDPPGTTTSQWSLVS
                     GPGGVTFADPTSPTTTASFSTDGTYVLRLTADDSAARSSDDTTITVLPEGTVRPTITE
                     AGVAQSSDDAEERPSGSVSLTSSDLELVVDGSNQQTVGIRFAGTQIPAGATIQDAYVQ
                     FTVDEVSTGADTLTIRGQAADDPGTFTTATGNISSRPRTAAAVSWSPPPWDTVGQAGT
                     GQRTPDLAAVLQEIVDRPGWASGNALALIVTGTGTRTAEAFDGTAIPILHVEYTTGSA
                     SNTAPVVNSVGITPSSPATNDTLSAIVDASDADGDPLSLSYRWAVNGSDLAGQTSSTL
                     DLSVPGNGDAGDQISVTVVASDGIADSAPVSSAPVTVVDSPPAPPGAPTGLTATLSTA
                     AVELSWDPNPEPDLAGYEVYRSGSATGPYTKLNGSLLTTPAYADPAAPVGATSYYQVV
                     AVDAAGNPSAPASTSATRTIAVVGASTASANHGDALAIATPQGTAPGDLMLASITVAG
                     SPAVTAPTGWTRVQAATAGGGQLESVLYAKVAAPGEPASATFALSARHSVVGQIVAYR
                     GVDPTAPIDASAAVANPTSQAIVAPSVSATGPGEVLVSTFAIDANTTIQPPDGMLEQA
                     EVAAAGRGKVAAETADQVLGSSGDTGTRTATASSSALSIGISVTLRPA"
BASE COUNT          697 a         1713 c         1479 g          633 t
ORIGIN      
        1 gtagccagta cctgtcgagg gcccgggcga cctcctcgtc ccgggacagg tcgctgacag
       61 cctgctgctc cagcacctcg ggaaccccgg cctggatcct gcaccagagc tgcttccgca
      121 caaccggcga catcgcaccc gcagcgccca ccggtgcccc ggcagcgagc accccctggc
      181 cgaccgcccg gtccgcatac ccaagcgccg ccttcgccgg ttcctcaacc gccttgtcgc
      241 cgtacccgag cacacgaccg gggcggccgc catcgtcgtg atccggagag ctcacaacga
      301 ccggcgatcc gcatccgctg agggcggccg ccaaggccgg tcgccaccgc gctctggacg
      361 ctggaggcgg cgcatatcgt cactgcgcac tctggtcatc gtcgcgatgg ctcctgagtt
      421 gtcgcatgtt cgtacaccgg aacctgtaac gcaacaagac tgaccccggc attgatgatg
      481 cgcttctggt gtgatgtccg cattgcgcgg gacaacactg aagcatgatc cagccgagtt
      541 atccgatgaa gcccagattc aggctgtccc acgcgagccc tacagttcct ccaactgagc
      601 aggcgcggcg ccagcggcgt acgtcatcta cgtcgagcgc gggatgaagc gttctttgag
      661 gggaagtacc ctcctcatcg ttccctcatg atcgccgcga accacttggc gacacggcaa
      721 cgacgcaggt tccgaccgtg cggaggcccg cttcgggccg atgcgttcga cagctccgat
      781 caggggaagg gggcggcgca acacttgcgt tcgacgggtc gcgcacttgt cgcatttttc
      841 gtggtcgcgg tgatgtcgtc ggcgatgcct gcggcaggcg cggccacgtc gaccccaacc
      901 tcgggccacg ggttgccgaa ggcccgggcg atccgatcca tcgcgacggc cgaatacggg
      961 gtcgcccggc ccgcgggtgt gacctacgtc cccgacgccg ggaccctcct ggttgcgggc
     1021 gaggtgctgc tggagctcac cccggccgac gatctggtgg ctacccacag ccttccggcc
     1081 cgcttcgatg ccgccacgct cgccttcgat ccggccttcg ggcgactgac gtccgtgacg
     1141 gatggccagc tgctcaccgg aagaccgcag ggtctctcgg acgtccgaac gctcgacctg
     1201 cgccgactcc aactcggtga cccggcgggg acgaccttcg accccgagag cggggactgg
     1261 ttcatcctgg accttcgcgg gccctcgctg gtgagggtgc ggtcgcagcg tgacctgtcc
     1321 accgcccccg tgagccggat ccccatgcgg ccgctcgggt ccggcggact gcgggggctc
     1381 gcctacaacc ccgcggacgg tcttctctac accgcggggc agggtggcga cggcgacctc
     1441 ctgtacgcga tcgacgactc cgggacggtc cgcggcacct acgatctgtc ggagaccggc
     1501 atccgcgacc tgcgtgccat ggtcttcgcc cccagcgccg atcccacgga cgacccttcc
     1561 accgtgcatc tgtacgtggc cgacgccggc ggctcggcca cgtacggcag cgtcacggaa
     1621 gtgacgctcg aggccgctgt cacggccgcg gccgccaccg atgtcgcgac cctcgtccag
     1681 aggatcgaca cctcgcagtt cacacctgcg agccccgacc cctccggtgt cgtgtacctg
     1741 ccggcggagc gtcgcctcgc gatcaccgac tcggaggtcg acgagacgac cggggcgggc
     1801 taccacgggg tcaacctctg gcaggtcacg tcgaccggct cggtccagga tacggggacg
     1861 acggtcccct tctcgataga gccgaccggg ctgggatacg accccgcgag caggacgctg
     1921 ttcatctccg acgacgacaa gaaccgggtg ttcctcgacc ggcccggtcc cgacggcagg
     1981 ttcggcactt ccgacgacga cgtgagctgg atcgacgtgg gtgcctacag cacggatgcc
     2041 gaggacccgg agttcgatcc cgccaccggg catctgttct tcatcgacgg cgtggccagg
     2101 gagatcttcg agatcgaccc ggtgaacggc gtcttcggcg actccgacga ccaggtctca
     2161 agcttcgacc tggcgcggta cggcgccaac gacctcgagg ggctcgggtc ggatcccgcg
     2221 cggaacacct tgctcgcggg cgagcggagc gccaagctga tctacgagct gaccaaggac
     2281 ggggcgttgg tccgcaccat cgacgcctcc ggcatcagcg ggatggggca catctcgggc
     2341 ctggccatgg cgccggcgag ctacgacgcg tccaggatga gctactggat cgtcgaccgc
     2401 ggcaccgaca acgggagcaa cccgaacgag aatgacgggc agctgttcga gatcagcgcc
     2461 tcgacgtcgg tgaacgcgcc accggcggtc gatgccggct cggaccagac ggtcgtccag
     2521 cccgaccggg cgaccctggc gggggtcgtc agcgacgatg ggctgcccga tcctccgggg
     2581 accacgacca gccaatggtc cctcgtgagc ggtcccggcg gcgtcacctt cgccgatccc
     2641 acgtccccca ccacgacggc ctcgttctcc accgacggca catacgtgtt gcggctcacc
     2701 gccgacgatt ccgcggcgcg aagctcggac gatacgacca tcaccgtcct tcccgagggg
     2761 accgtgcgtc ccaccatcac ggaagccggg gtagcccaga gctcggacga cgccgaggag
     2821 cggccctcgg ggtcggtgag cctgacgagc tcggacctgg agctcgtcgt cgacgggtcc
     2881 aaccagcaga cggtcgggat ccgcttcgcc gggacccaga tcccggccgg ggcgacgatc
     2941 caggacgcct acgtgcagtt caccgtggac gaggtctcga cgggcgcaga caccctgacc
     3001 atccggggcc aggcggccga tgaccccggc accttcacca cggcgaccgg caacatctca
     3061 tccaggcctc ggacggctgc cgccgtgagc tggtccccac ctccatggga cacggtgggc
     3121 caggccggaa ccggccagcg gacccccgac ctcgcagccg tgctccagga gatcgtggat
     3181 cgtcccggtt gggcttccgg gaacgcgctg gccctgatcg tcacgggtac cgggacccga
     3241 acggctgagg ccttcgacgg aacggcgatc ccgatcctgc acgtcgagta cacgaccgga
     3301 tccgcctcga ataccgctcc ggtggtgaac tcggtgggca tcacgcccag ctcgccggcc
     3361 accaacgaca ccctcagcgc aatcgtggac gcctccgacg ccgacggcga cccgctcagc
     3421 ctgtcctacc ggtgggccgt caacggcagc gacctggccg ggcagacctc ctccaccctg
     3481 gacctgtcgg tgcccggcaa cggtgacgcc ggcgaccaga tctcggtcac ggtggtggcc
     3541 tccgatggga tcgccgactc ggcgccggtg agctcggccc cggtcaccgt ggtcgactcc
     3601 ccaccggccc cgcccggggc ccccaccggc cttacggcca ccctttccac cgcggcggtc
     3661 gagctgtcct gggacccgaa ccccgagccc gacctggccg gctacgaggt gtaccgctcg
     3721 ggcagcgcca ccggcccgta caccaagctg aacggctccc tgctcaccac cccggcctac
     3781 gccgaccccg ccgccccggt gggggccacc tcctactacc aggtcgtggc ggtggatgcg
     3841 gccgggaacc cctcggcgcc cgcctcgacc agcgccaccc gcacgatcgc cgtggtgggg
     3901 gccagcaccg ccagcgccaa ccacggcgac gccctggcga tcgccacccc ccaaggcacc
     3961 gcccccggcg acctgatgct ggcctccatc accgtggccg ggagccccgc cgtcaccgcc
     4021 cccaccgggt ggacccgggt gcaggccgcc accgccggtg gggggcagct ggagagcgtg
     4081 ctctacgcca aggtggccgc ccccggcgag cccgcctcgg ccaccttcgc cctgtcggcc
     4141 cgccactcgg tggtgggcca gatcgtggcc taccgggggg tggaccccac cgcccccatc
     4201 gacgcctcgg ccgcggtggc caacccgacc tcgcaggcca tcgtggcccc ctcggtgtcc
     4261 gccaccgggc ccggggaggt gctggtgagc accttcgcga tcgatgccaa caccaccatc
     4321 cagccgccag acgggatgct cgagcaggcc gaggtcgccg cggcgggcag gggcaaggtc
     4381 gccgccgaga ccgcggacca ggtgctgggc tcctccgggg acaccgggac ccggaccgcc
     4441 accgcctcct cctcggccct cagcatcggg atttccgtca cgctgcggcc ggcctgagcc
     4501 agcccgccac gctggcgatg aa
//
LOCUS       DASQQQ010000070         6057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_253940, whole genome shotgun sequence.
ACCESSION   DASQQQ010000070 DASQQQ010000000
VERSION     DASQQQ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253940"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="VE646_03000"
     CDS             complement(<1..1310)
                     /locus_tag="VE646_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZD78997.1"
                     /translation="MARVAAYGSWRSPIGADLVARAGVVLSEPWLTAEGVFWLELRPR
                     EGGRSVVVRADASGAPTDVTPAGFDVRTKVHEYGGGSYLVHDATVFFSNFVDQRLYRQ
                     EPGTPPEPITPAPPSPGSIRYADGRITPDGSTIVCVRERRQGDETHNDLALVPADGSG
                     PPLPIASGRDFYAFPRIAPDGSALAWIEWDHPNMPWDGTELHVASLSADGLSEDQVVA
                     GGERESIFQPAWTPAGELCFVSDRTGWWNLYRRGGEGPQALAPMDAEFGVPMWEFGYS
                     GYDLLDDGRIVCAYRANGIHHLAILDPLSGELLDLDIPYTCFDPPYVRARGSQVVFVG
                     GGPTTPDQVVALDLDARSVDVLRESEQLSIDPAYLSVPRPIAFPTEGGLTAFGYHYPP
                     ANPDHRPPEGELPPLIVHIHGGPTAESTPELSLGKQFWTSRGFAV"
     gene            complement(1311..2168)
                     /locus_tag="VE646_03005"
     CDS             complement(1311..2168)
                     /locus_tag="VE646_03005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HZD78998.1"
                     /translation="MKASELLEQGIRTLKASPAIDHWQKDREEIEAEDLLGYVLGRDE
                     VDRDAEVLPAAARRFGRLVARRAEGEPVQLIKGYAEFRGLRMRSRPGVFVPRDSTEFL
                     AEQAIRRLRRRRRPVAVDLATGGGTVALAIANETKGARVYGTDISDVAIHVADRNARE
                     LRLRATFLVGDLFAALPAPLRGVVDVITLHPPYVARAEMRDLPDEIRRFEPAHTLSDN
                     SVDGLSLIQRTASESRAWLRRGGWLLVEVSPDRARSVSAVLRRNGFRDVRSTKGGELQ
                     VTRVVVGRR"
     gene            2209..3399
                     /locus_tag="VE646_03010"
     CDS             2209..3399
                     /locus_tag="VE646_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012014035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZD78999.1"
                     /translation="MDVRDAPDEAAFRQRLRSWLHSNLPEGWGTPGYVEPSGPEGLAF
                     QKQWSRRLYEAGFIGLTWPKAYGGFGAPPAYQVILMEELGRAGAPEHATPIGLGMAGP
                     AILTHGTEEQRRRYLPPILTGQEVWCQGFSEPEAGSDLASLRTRAERDGDAYVISGQK
                     VWSSFAHQADLCILLARTDPEAERHAGLTYFIVPMHAPGVEVRPLRQITGDAEFNEIF
                     LDGVRVPVENVVGAPGDGWTVAVNTLMHERANLAVMLTVRLDDALTRLIALARSTRRH
                     GGRAADDPLIRDAIAGLWSDVQALRVTNARALSMLTSTGAPGPEGSIVKLVWAEANQR
                     LTSLALEIQGSYAMLSGGEHAVDGGRWQRLQLRSRGNSIEGGTSEILRDVIAERILDL
                     PKSR"
     gene            3407..4525
                     /locus_tag="VE646_03015"
     CDS             3407..4525
                     /locus_tag="VE646_03015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZD79000.1"
                     /translation="MDLTETETHRLLRDQARAFLGVRYPPERVAGIADGKGFPPDEWR
                     SIVELGWTSISVPETSGGAGLGLPEEAVLAEELGRVVFPGPFLSTVVMALPALAADPD
                     LQRAVTSGRSIATLAWAGPDGAFRTDRFPIGVGKGTDLHHLYGSTWFVPDLAIADLAV
                     VTGRGPRGPGVWAVRLEGDSVSRQPLPTVDGTRRMGALFLEGAEGRLLAEGDEAVCLL
                     GRIRERTLAVLSMEGVGVASAALELAVAHARTRVQFGRPIGAFQAVSQQLASAYADLE
                     GARSLALWAAAAVAAGEQDAPRAAAAAKARSSEAAVGACERAIQVLGGMGFTWEHPLH
                     RLYRRAEWDRAFMGWPSELRASVAASLLDDPEPQGWRR"
     gene            4535..4903
                     /locus_tag="VE646_03020"
     CDS             4535..4903
                     /locus_tag="VE646_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZD79001.1"
                     /translation="MARPSAARSTAPSSRPPSVRGVRLTADRLVVRQPDEGERTSRGG
                     LLIPATAAPAPRRCLWGEVTLVGPDVRGVKVGDRVLFLPQAGLEVELEGEDRLLLRER
                     DVQAVASPTEGPEQSPGQYL"
     gene            complement(4906..5310)
                     /locus_tag="VE646_03025"
     CDS             complement(4906..5310)
                     /locus_tag="VE646_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009955914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HZD79002.1"
                     /translation="MRTTEVEIDTTGRRVIDVTEHVRRFVAEGGEDGIVLVFLPHATA
                     GVALMETGSGSEPDLEDLVERLLPRDERYRHRHGSVGHGGDHLLPVLVAPSLVLPVEA
                     GRLVLGTWQSLVVVDPNRENDRRRLRLQVLAG"
     gene            complement(5307..>6057)
                     /locus_tag="VE646_03030"
     CDS             complement(5307..>6057)
                     /locus_tag="VE646_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79003.1"
                     /translation="GRGRDRRGRGAMTRSDRSGLAGILAVMGGSIAALGSLMEWAQAT
                     GLGFRASARGLSGWEGKVTLLAAGLVLFAGLGVLAGSGVGRLRLPAAFGGLVITGVAV
                     ATALTARAQVIEGAARQISRRLNLPLEQTRDAVRGTFESGALRIGLATGLYLVVAGGI
                     LALGAAALAHRAVGRAALGSASGGGSLAGSGGRPGWPAPPPAGSSGRGPRPAGPQPAV
                     GRVGPTVLPPPPPPRGPDGRLDPRGPMGGEP"
BASE COUNT          856 a         2238 c         2147 g          816 t
ORIGIN      
        1 accgcgaacc cccgactggt ccagaactgc ttgcccaggg agagctcggg ggtggactcg
       61 gcggtgggtc ctccgtggat gtggacgatc agcggcggca gctcgccctc gggcggccgg
      121 tggtccgggt tcgccggtgg atagtggtac ccgaaggcgg tgagccctcc ctcggtgggg
      181 aaggcgatgg gtcgagggac cgagaggtac gcggggtcga tgctcagttg ctcgctctcg
      241 cggagcacgt ccacgctccg cgcatccagg tccagcgcca ccacctggtc cggagtcgta
      301 ggaccaccgc ccacgaagac cacctgagaa ccccgagccc ggacgtacgg cggatcgaag
      361 cacgtgtagg gaatgtcgag gtcgagcagc tcgccggaga gcggatcgag gatcgccagg
      421 tgatggatgc cgttggcgcg gtaggcacac acgatccgtc cgtcgtcgag gaggtcgtac
      481 ccgctgtagc cgaactccca catcggcaca ccgaactcgg catccatggg cgccagcgcc
      541 tgcggcccct ctcctcctcg tcggtacagg ttccaccaac cggtgcgatc agagacgaag
      601 cagagctcgc ctgccggcgt ccaggcgggc tggaagatcg actcgcgctc ccccccggcc
      661 accacctggt cctccgagag cccgtcggca gaaaggctcg ccacgtggag ctcggtgcca
      721 tcccagggca tgttggggtg gtcccactcg atccacgcga gggccgagcc gtcgggggcg
      781 atccgaggga aggcgtagaa gtcccggccg gatgcgatgg gaagcggcgg ccccgagccg
      841 tcggccggaa ccagcgccag atcgttgtgc gtctcgtcgc cttggcgtcg ctcccgtacg
      901 cagacgatcg tcgatccgtc cggcgtgatc cggccgtcgg cgtaccggat cgaccccggg
      961 gacggcggcg cgggggtgat cggctccggg ggggtccccg gctcctgtcg gtacaggcgc
     1021 tgatcgacga agttcgagaa gaacaccgtg gcgtcgtgga ccaggtacga cccgccaccg
     1081 tactcgtgca ccttggtccg gacgtcgaac cctgcgggcg tcacgtccgt aggggcgccc
     1141 gacgcatcgg cccggaccac cacgctccgc ccgccctccc ggggccggag ctccaaccag
     1201 aacacgccct ccgcggtcag ccacggctcg gagagcacca cgccggcccg agccaccagg
     1261 tcggcgccga tcggcgagcg ccacgaaccg tacgctgcga ccctcgccat ctaccgcctc
     1321 cctacgacca cgcgggtcac ttggagctcg ccacccttcg tgctgcgcac gtcacggaaa
     1381 ccgttgcgcc gaagcaccgc ggacaccgaa cgggcccggt cgggactgac ctccaccagc
     1441 agccaccccc cacgccggag ccaggctcgg gactcggagg cggttcgctg tatcaggctc
     1501 aggccgtcga cgctgttgtc gctcagggtg tgggcgggct cgaaccggcg gatctcgtcg
     1561 gggaggtccc gcatctccgc tcgggccacg tacggcggat gcagggtgat gacgtccacc
     1621 accccgcgca gcggggccgg aagggcggcg aacagatcgc ccaccaggaa cgtggcccgc
     1681 agacggagct cgcgggcgtt ccggtcggcg acgtggatcg cgacgtcgga gatgtcggtg
     1741 ccgtacaccc gggccccctt cgtctcgttc gcgatggcca gcgccaccgt tcctcctccg
     1801 gtggccagat cgaccgccac cggccgccga cgccggcgca accgacggat ggcctgctcg
     1861 gccaggaact ccgtggagtc gcgggggacg aacacccccg gccgggagcg catccgcagg
     1921 ccccggaact ccgcgtagcc cttgatcagc tgtaccggct ctccctccgc ccggcgagcc
     1981 accagccgtc cgaaccggcg ggcggccgcc gggagcacct cggcatctcg atccacctcg
     2041 tcccgaccca ggacgtagcc caacaggtcc tcggcctcga tctcctcccg gtccttctgc
     2101 cagtgatcga tcgcgggcga ggccttcagc gtccggatcc cttgctccag cagctcggat
     2161 gccttcatcg cggcaggcat cctaccggtg cgagcgtaag ctgaccgtgt ggatgtccgc
     2221 gatgccccgg acgaagcggc cttccgccaa cgcttgcgct cctggctgca cagcaacctc
     2281 cccgagggat gggggacgcc gggctacgtc gagcccagcg gcccggaagg cctcgcgttc
     2341 cagaagcagt ggtcccggcg gctgtacgag gcggggttca tcgggctcac ctggcccaag
     2401 gcctacgggg ggttcggcgc accgcccgcc tatcaggtca tcttgatgga ggagctgggt
     2461 cgcgccggtg cccccgagca cgccacgccg atcggactgg ggatggccgg ccccgccatc
     2521 ctcacccacg gcacggagga acagcgccgg cggtacctgc cccccatcct gaccgggcaa
     2581 gaggtctggt gccaggggtt ctccgagccc gaagcgggct cggacctcgc ctcgttgcgg
     2641 acccgggcgg agcgggacgg cgacgcctat gtgatcagcg gccagaaggt ctggtcctcg
     2701 ttcgcgcacc aggccgacct gtgcatcctg ctggcccgga ccgatccgga ggcggagcga
     2761 cacgccggcc tcacctactt catcgtgccg atgcatgccc cgggggtgga ggtccggccg
     2821 ctgcgccaga tcacgggaga cgccgagttc aacgagatct tcctggatgg ggtgcgggta
     2881 ccggtcgaga acgtggtggg cgctccgggc gacggatgga ccgtggccgt caacaccctg
     2941 atgcacgagc gggcgaacct ggccgtgatg ctgacggtgc gcctggacga cgcgctgacg
     3001 cggctgatcg cgctggctcg gagcacccgg cggcacgggg gtcgggccgc cgacgaccca
     3061 ctgatccgag acgcgatcgc cggcctgtgg tcggacgtgc aggcgctccg ggtgaccaac
     3121 gcccgggccc tgtcgatgct cacttccacc ggggctccgg gccccgaggg gtcgatcgtc
     3181 aagctggtgt gggccgaggc caaccagcgg ctgacctccc tcgccctgga gatccagggc
     3241 tcgtacgcga tgctctcggg cggggagcac gccgtggacg gcggtcggtg gcagcgcctc
     3301 cagctccgta gccgcggcaa ctcgatcgag ggaggcacct cggagatcct gcgcgacgtg
     3361 atcgccgagc gcatcctgga cctgcccaag agccggtgag cggaccgtgg acctgaccga
     3421 gaccgagacc caccggcttc tccgcgacca ggctcgcgcc ttcctcgggg tccgttaccc
     3481 cccggagcgg gtggccggga tcgccgacgg caagggtttc ccccccgacg agtggcgctc
     3541 gatcgtcgaa ctcggatgga catccatctc ggtgccggag acctccggtg gagccggtct
     3601 ggggctgccc gaggaagccg tgctggccga ggagctcggc cgggtggtgt tccccgggcc
     3661 gttcctgtcc accgtggtga tggccctgcc cgccctggct gccgacccgg acctgcagcg
     3721 agcggtgacc tcgggtcggt ccatcgcgac gctcgcgtgg gcaggcccgg atggcgcctt
     3781 ccgcaccgac cggttcccga taggggtggg caagggcacg gacctccacc acctctacgg
     3841 gtcgacgtgg ttcgtcccgg acctggccat cgccgacctg gcggtggtga ccgggcgcgg
     3901 tccacgaggt ccgggggtct gggccgtgcg gctcgaaggt gactcggtct ctcggcagcc
     3961 actccccacc gtggatggca cgcgacggat gggagccctg ttcctggagg gcgcggaggg
     4021 ccgcctgctc gccgaaggcg atgaggccgt ctgcctcctg ggtcggatcc gcgaacgcac
     4081 cttggcagtc ctgtccatgg agggcgtcgg cgtcgcgtcc gccgcgctgg agctggccgt
     4141 ggctcacgct cggacccggg tgcagttcgg gcggccgatc ggagcgttcc aagccgtgtc
     4201 acagcagctg gcgtcggcgt acgcggacct cgagggggct cgctcgctcg ccctctgggc
     4261 ggcggccgcc gtcgccgccg gcgagcagga cgccccccgt gcagcggccg ccgccaaggc
     4321 ccggagctcg gaagccgccg tcggggcgtg cgagcgggcc atccaggtgc tggggggtat
     4381 ggggttcacc tgggagcatc ctctccaccg cctgtacagg cgggcggagt gggatcgggc
     4441 cttcatgggg tggccgtcgg agctgcgggc gtccgtggcc gcgtccctgc tggatgaccc
     4501 ggagccccag ggatggcgcc ggtagactga ccgggtggct cgaccctccg ccgccagatc
     4561 gaccgccccc agttcacgcc ccccgtcggt ccggggcgtc cggctcaccg cggaccggct
     4621 cgtggtgcgg caaccggacg agggggaacg gacctcccgg ggcgggctcc tgatccccgc
     4681 caccgcggcc ccggcccccc gccgctgtct gtggggcgag gtgacgttgg tcggtccgga
     4741 cgttcgcggg gtcaaggtgg gtgaccgggt cctgttcctc ccgcaggccg ggctcgaggt
     4801 ggagctcgag ggagaggacc gtctgctcct gcgggaacgc gatgtgcagg cggtggcctc
     4861 gcccaccgag ggcccggaac agagccccgg ccagtacctc tgaggtcaac cggcgaggac
     4921 ctggagccgc aggcggcgcc gatcgttctc gcgattggga tcgaccacca ccaggctctg
     4981 ccaggtgccg aggaccagac gccccgcctc cacgggaagc acgagggacg gggcgaccag
     5041 gaccggcagg aggtgatcgc ccccgtggcc gacggacccg tgccgatgcc ggtatcgctc
     5101 gtcgcggggc aggagccgct ccacgaggtc ttccagatcg ggctcggagc ccgatcccgt
     5161 ctccatcaag gccacgccgg ccgtggcgtg gggcaggaac accaggacga tgccgtcctc
     5221 tccgccctcg gccacgaacc ggcgcacgtg ctcggtgacg tcgatcaccc gccggccggt
     5281 ggtatcgatc tcgacctccg tcgtcctcac ggctctcccc ccatcggtcc cctgggatcc
     5341 aaccgcccgt ccgggccccg gggtggtgga ggcggcggca ggacggtcgg ccccacccgt
     5401 ccgaccgcag gctgaggccc tgccggccgc ggcccgcgtc cgctcgatcc cgccggcgga
     5461 ggagccggcc agccgggccg accccccgac cccgcgaggg acccgccccc cgacgcgctc
     5521 cccagagccg ctcgccccac cgcccgatga gccagcgcgg ccgccccgag ggcgaggatc
     5581 ccgccggcca ccaccaggta caggcccgtg gccaggccga tgcgcagtgc ccccgactcg
     5641 aacgtgccac ggaccgcgtc ccgcgtctgc tcgagcggca ggttcaggcg gcgggagatc
     5701 tgccgtgccg ctccctcgat cacctgggcc cgtgcggtca gggccgtggc cacggccacc
     5761 ccggtgatca ccagcccacc gaacgccgcc ggcaaccgga gacgccccac cccggagccg
     5821 gccagcaccc ccagaccggc gaagagcacc agaccggcgg ccaggagcgt gaccttgccc
     5881 tcccacccgc tcagaccccg ggccgaggcc cggaagccga ggcccgtggc ctgtgcccac
     5941 tccatcagcg agcccagcgc ggcgatcgac ccacccatca cggccaggat ccccgccagc
     6001 cccgatcggt ccgatcgtgt catcgccccg cgtcccctcc ggtcccggcc cctgccg
//
LOCUS       DASQQQ010000071        10262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_336174, whole genome shotgun sequence.
ACCESSION   DASQQQ010000071 DASQQQ010000000
VERSION     DASQQQ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10262
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336174"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VE646_03035"
     CDS             <1..447
                     /locus_tag="VE646_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79004.1"
                     /translation="DVGQFFSIEEIGLGPNGWVGLLNYTDAPASLDTLFLCQPPRCVE
                     LPDVLVRPGHVARVTVGDGSGLERVVMTDAPLDLAPSDGEVALYSTDDVDDADRLWAY
                     LEWGSTPHAGTEMAIEAGLWHVGSYAPSSDDATRLYRTDDGLWVFE"
     gene            complement(574..1227)
                     /locus_tag="VE646_03040"
     CDS             complement(574..1227)
                     /locus_tag="VE646_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79005.1"
                     /translation="MAAQQPEATRSGDAQREPAFQTVRRGYDPEEVRAHVAKLTDRLE
                     ALSSQVTRTQRLLDEARRERDLARDAPRADSYKEVSTHVVDLIRRFDQDVAGLRRQAE
                     AEAIGILADARSEAAQLRMQANAEEQEARTRVDEMLGSARLETERLKHELEPVRAATL
                     SRIREMRDGLAASVQELDRVLGAEREQVVVVGEPDDGDPTGGAMPGPPPEARSELGT"
     gene            complement(1362..2072)
                     /locus_tag="VE646_03045"
     CDS             complement(1362..2072)
                     /locus_tag="VE646_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79006.1"
                     /translation="MATEGSGFGSAASGDRSPPSFDMVKRGFDPDQVLQYLRGVAQRV
                     QALEDQIRGLEREREEAKRERDIALEAWETARKDPYEALAAHVAELVRHFDAEVEKLR
                     GEAELEAARILSGAREEADRIRQQAGGAQAEARGQADHIRRQAREEAERILGDAKAQT
                     DQIKSHLMTLHGSTLNELRIIRDHMRNSVREIDVILNGGQQQEEERVVVLGHAGEAEK
                     PVREDEPSARRQETPFGQ"
     gene            complement(2159..2920)
                     /gene="map"
                     /locus_tag="VE646_03050"
     CDS             complement(2159..2920)
                     /gene="map"
                     /locus_tag="VE646_03050"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003391937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZD79007.1"
                     /translation="MIDYKSREDLDRMRKAGRIVASTIERVLEAVRPGATTADLDAVA
                     EAHIVERDAIPSFKGYGQPPFPASICASVNDEVVHGIPSPRRVLREGDLLSLDFGAIW
                     EGFHGDSAVTVFVGEPPSPEAEKLVRVTEEALEAGIAQIRPGGRLSDVGAAVQQVAEG
                     AGFQVVREYVGHGIGRNMHEDPQIPNYGEPGRGPELKPGLVIAVEPMVNVGGWETRVL
                     ADHWTVVTADGSLSAHFEHTIAVTEDGREVLTARA"
     gene            complement(2917..3579)
                     /locus_tag="VE646_03055"
     CDS             complement(2917..3579)
                     /locus_tag="VE646_03055"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013429981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HZD79008.1"
                     /translation="MRIILLGPPGAGKGTQARRLAERYGLSTIATGDIFREQIANATP
                     LGLQAKEYVDRGEYVPDDITTEMVLERLDRPDAREGFILDGFPRTVPQAQALERALSQ
                     AGRPLHAVLNIKISDEMAVKRLMARLVCPNCKRSYNLEFKPPRVEGTCDVCGHELVNR
                     SDDDEATIRRRLEVYHRETQPLVLYFWERGLLRDIDAEAREEVVADRTVEAIADLTED
                     FG"
     gene            complement(3590..4882)
                     /gene="secY"
                     /locus_tag="VE646_03060"
     CDS             complement(3590..4882)
                     /gene="secY"
                     /locus_tag="VE646_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006539152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZD79009.1"
                     /translation="MLRAFVNAFRVPDLRNKILFTLLVIALYRLGSHVPVPVVDISIL
                     QQAAEQQGATGFLSFIDLFSGGALTRMAVFGLGIMPYITASIIMQLLTVVIPKLERWQ
                     KEGESGTKKINQWTRYVTVVLALLQSTGLVFLFHSPSSTTGLGVDIFPAGEFTAQNIA
                     LIVLVMTAGTATIMWMGELITQRGIGNGMSILIFTSVISRLPQEGRSILLSGGTTKFT
                     VVLLIGMAIVVAVIFIEQGQRRIPVQYAKRVVGRRMTAGGSTYIPLKVNQAGVIPIIF
                     ASSVLYFPTLLASVYHAVWFQNFVNDYLTNQRSMVYMTFYGLMVVFFAYFYTAITFNP
                     VDTADNLRKYGGFIPGIRPGPPTSDYLSKVLSRITLPGSLFLATIALLPSIVFAFWVI
                     QQFPFGGTSLLITIGVALETMKQLESQLLMRHYEGFLR"
     gene            complement(4904..5341)
                     /gene="rplO"
                     /locus_tag="VE646_03065"
     CDS             complement(4904..5341)
                     /gene="rplO"
                     /locus_tag="VE646_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HZD79010.1"
                     /translation="MKLHHLRPAEGAKRERKRVGRGRAGVRGKTAGRGTKGYLARHNP
                     KTGFEGGQMPLQRRVPKLKGFTNPNRLEYAVVNVETLGKVFDGGEVDPAALHAHGLVG
                     KGRKVKILARGELGTALTVRAHAFSEAARAKIERAGGRAELIG"
     gene            complement(5395..5574)
                     /gene="rpmD"
                     /locus_tag="VE646_03070"
                     /pseudo
     CDS             complement(5395..5574)
                     /gene="rpmD"
                     /locus_tag="VE646_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119225.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
     gene            complement(5571..6227)
                     /gene="rpsE"
                     /locus_tag="VE646_03075"
     CDS             complement(5571..6227)
                     /gene="rpsE"
                     /locus_tag="VE646_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010145303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HZD79011.1"
                     /translation="MRRYDSQGEKSIYEERVIGINRVAKVVKGGRRFSFAALVVVGDG
                     AGRVGLGYGKAKEVPAAINKGVEEAKRKMFEVPMMGTTIPHQVIGEDGAGAVLLKPAA
                     PGTGVIAGGPVRALVECAGIKDILSKSLGSANAINIVKAAMAGLKSLKRPEEVARLRG
                     RDVHQVAPKPMLEAVAAAQERQQATRAAKLEEVYGDQAAASPGPQRSGPRTGPRGGRR
                     "
     gene            complement(6227..6583)
                     /gene="rplR"
                     /locus_tag="VE646_03080"
     CDS             complement(6227..6583)
                     /gene="rplR"
                     /locus_tag="VE646_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HZD79012.1"
                     /translation="MEAKTKRGARLRRHHRVRRKIAGTPTRPRLAVYRSNRHIYAQLI
                     DDDAARTLIAASDRELPAAGGPGKTGAAKAVGELLAERAKAAGIDRVVFDRGGRLYHG
                     RVAALAEGAREKGLEI"
     gene            complement(6583..7134)
                     /gene="rplF"
                     /locus_tag="VE646_03085"
     CDS             complement(6583..7134)
                     /gene="rplF"
                     /locus_tag="VE646_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HZD79013.1"
                     /translation="MSRIGREPIEIPSGVDVALGDDAVVTVRGPRGTLSQRVHAEMRI
                     VREDSTLRVERPSDEGFHRSLHGLTRTLIANMVEGVTKGFEKRLAIVGVGYRAAMKGK
                     DLEVQVGYSHPVVVPCPEGIEFETPTPTSIVVRGNDKQQVGETAANIRKIRKPEPYKG
                     KGIRYENEYVRKKAGKAAKGAIA"
     gene            complement(7172..7585)
                     /gene="rpsH"
                     /locus_tag="VE646_03090"
     CDS             complement(7172..7585)
                     /gene="rpsH"
                     /locus_tag="VE646_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZD79014.1"
                     /translation="MVRLATHVTDPIGDMLTRIRNANLAYKDDLPLPASKMNRAILRI
                     LDAEGFIVGFEDEGEGIERVLRVHLKYGPKRERTITGLRRVSKPGRRVYVRRGELPRV
                     LGGLGIAIVSTSRGVMTDRDAARAGVGGEVLAYVW"
     gene            complement(7579..7764)
                     /locus_tag="VE646_03095"
     CDS             complement(7579..7764)
                     /locus_tag="VE646_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HZD79015.1"
                     /translation="MAKKALINKQERQAKFRVREYTRCRRCGRSRAVYRKLLLCRICF
                     RELAHAGELPGVTKASW"
     gene            complement(7780..8337)
                     /gene="rplE"
                     /locus_tag="VE646_03100"
     CDS             complement(7780..8337)
                     /gene="rplE"
                     /locus_tag="VE646_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007271040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HZD79016.1"
                     /translation="MKVRFREEARPRLQGELGMSNPLQVPRLEKVVLNMGVGDALKDG
                     RMLEAAVDDLAVITGQRPVITKARKSIAGFKVREGMAIGVKVTLRGDRMWEFVDRLVS
                     VAIPRIRDFRGLDPAAFDGHGNYTLGLTEQLIFPEIDYDKVVKVRGMDITVVTTARTD
                     EEGRALLVALGFPFQGAPMAGEAES"
     gene            complement(8361..8699)
                     /gene="rplX"
                     /locus_tag="VE646_03105"
     CDS             complement(8361..8699)
                     /gene="rplX"
                     /locus_tag="VE646_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HZD79017.1"
                     /translation="MPGVDIKKDDQVRVMNGKSRGHTGRVIRVLPREGRVLVEGAAMA
                     KKHQRTQGKRSASGSQLQQGGIIDTELFIDLSNVQLVCRSCGQPTRVGRRFEPDGSKV
                     RICRKCGEDL"
     gene            complement(8700..9068)
                     /gene="rplN"
                     /locus_tag="VE646_03110"
     CDS             complement(8700..9068)
                     /gene="rplN"
                     /locus_tag="VE646_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HZD79018.1"
                     /translation="MIQQETRCKVADNTGAKVVLCIRVLGGSHKRYARIGDVIVGSVK
                     DALPGGAVKKGDVVRAVVVRTAKERRRPDGSYIRFDDNAVVLINEQRNPRGTRIFGPV
                     ARELREKRFMKIISLAPEVL"
     gene            complement(9119..9397)
                     /gene="rpsQ"
                     /locus_tag="VE646_03115"
     CDS             complement(9119..9397)
                     /gene="rpsQ"
                     /locus_tag="VE646_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HZD79019.1"
                     /translation="MSDEQPAKPERGRRKVRTGVVVSDAMDKTVLVRIDRKVRHPLYK
                     KIVKRSSKLAAHDEANDAHVGDTVRVMETRPLSKTKRWRIVEIVERAK"
     gene            complement(9394..9627)
                     /gene="rpmC"
                     /locus_tag="VE646_03120"
     CDS             complement(9394..9627)
                     /gene="rpmC"
                     /locus_tag="VE646_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HZD79020.1"
                     /translation="MAKIREIRELGDEDLLERVESLKEELFNLRFQLATGQLDNPMRI
                     KQARHEAARILTILRERELEELEAVEDEEREQA"
     gene            complement(9627..10046)
                     /gene="rplP"
                     /locus_tag="VE646_03125"
     CDS             complement(9627..10046)
                     /gene="rplP"
                     /locus_tag="VE646_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HZD79021.1"
                     /translation="MLAPKKVKHRKVHRGRRRGRAKGGTEIHYGDYGLVALQPAWITN
                     RQIEAARVAITRHIRRGGKVWINIFPDKPYTKKPAETRMGSGKGNPEGWVAVVKPGRV
                     MFELSGVSEPLAREAMSRAAHKLPIKTKFISRMGGVE"
     gene            complement(10047..>10262)
                     /locus_tag="VE646_03130"
     CDS             complement(10047..>10262)
                     /locus_tag="VE646_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79022.1"
                     /translation="EIPQAELETERLRARALAQVSSGGASTGALITDEAEPAEPAAEE
                     AKPEAKPEAKPEAKPETKPETKPEEEG"
BASE COUNT         1319 a         3853 c         3384 g         1706 t
ORIGIN      
        1 gacgtcggac agttcttcag catcgaggag atcggcctcg ggccgaacgg ttgggtgggg
       61 ctcctgaact acaccgatgc gccggcgagc ctcgacacgc tcttcctgtg ccagcccccg
      121 cggtgcgtgg agctccccga cgtgctggtg aggcccggcc acgtggcgag ggtgaccgtc
      181 ggcgatgggt ccggcctgga gagagtggtc atgacggacg cccccctcga cctcgcccca
      241 tcggacgggg aggtcgccct gtactcgacg gacgatgtcg acgacgccga tcgcctgtgg
      301 gcctacctgg agtggggctc gacgccgcac gccggaaccg agatggcgat cgaggccggc
      361 ctgtggcacg tgggctcgta cgccccctcg tccgacgacg ccacgcgcct gtacagaacc
      421 gacgatggcc tctgggtctt cgagtaggcc tcaccccgtc tcgaactcgt ggcccggcac
      481 gagcgacccg tccgccgaaa cccggagatc gacaccacgc ggaagaggcg gcctctcggg
      541 gccgcctctt cgccttcccg gatcgagcgt gcgctacgtg ccgagctcgg accgggcctc
      601 cgggggcggg ccgggcatcg ccccgcccgt cggatcgccg tcgtcgggct cgccgaccac
      661 gaccacctgc tcgcgctcgg cgcccaggac ccggtccagc tcctggaccg aggccgccag
      721 gccgtcgcgc atctcccgga tccggctcag cgtcgccgcg cgcaccggct ccagctcgtg
      781 cttgaggcgc tcggtctcca ggcgcgccga gccgagcatc tcgtccacgc gggtccgggc
      841 ctcctgctcc tcggcgttgg cctgcatccg cagctgggcg gcctccgacc tggcgtccgc
      901 gaggatgccg atcgcctccg cctccgcttg ccggcgcagg ccggccacat cctgatcgaa
      961 acgccggatg aggtccacga catgcgtcga cacctccttg tacgagtcgg cgcggggcgc
     1021 gtcgcgggcc aggtcccgct cgcggcgggc ctcatcaagc agccgctggg tgcgcgtgac
     1081 ctgggagctc agtgcttcga gccggtcggt gagcttggcg acgtgggcgc ggacctcctc
     1141 ggggtcgtat ccacgccgga cggtctggaa cgcgggctcg cgctgggcat cgcccgatcg
     1201 cgtggcttcc ggctgttgtg cagccatcgt ccctccggtg ggtcggggaa gggtactccc
     1261 ttccgtcccc cgaacgtacc gcgctcggcc tacggagggg agggcccgaa cggtcgtttc
     1321 tccccgcggg acggcctagg gcagaagtcg tagctccggg gctactggcc gaacggggtc
     1381 tcctggcgcc gggcggacgg ctcgtcctcc cgcaccggct tttcggcctc cccggcgtgc
     1441 ccgagcacca cgacccgctc ctcctcctgc tgctgcccgc cgttcaggat gacgtcgatc
     1501 tcgcgcacgg agttgcgcat gtggtcacgg atgatccgga gctcgttcag cgtggagccg
     1561 tggagcgtca tgaggtggga cttgatctga tcggtctgcg ccttcgcgtc gccgaggatc
     1621 cgctcggcct cctcccgggc ctggcgcctg atgtggtcgg cctgcccacg cgcctcggcc
     1681 tgggcccccc cggcctgctg gcggatccgg tcggcttcct ctcgggcccc ggagaggatc
     1741 cgcgccgcct ctagctcggc ttcgccgcgg agcttctcga cctccgcgtc gaagtgccgt
     1801 accagttccg ccacgtgagc ggccagggcc tcgtaggggt ccttgcgggc cgtctcccac
     1861 gcctcgagcg cgatgtcgcg ctcgcgcttg gcctcctcgc gctcccgctc cagcccgcgg
     1921 atctgatcct ccagggcttg aacgcgctgc gccacccccc gcaggtactg gagcacctgg
     1981 tcagggtcga agccccgctt gaccatgtcg aagctgggcg gcgagcggtc gcccgacgcc
     2041 gcagacccga agcccgatcc ctccgtcgcc atcgaacatc ccccctgcga actgtatgac
     2101 cgtcagcgtg caccgtagcg aatccgggtc cggcgcgcca gggcccgaag gtcggtcgtc
     2161 aggcccgagc ggtgagcacc tcgcgcccat cctcggtgac cgcgatggtg tgctcgaagt
     2221 gggccgagag cgacccgtcc gccgtcacca cggtccagtg gtcggccagg acccgcgtct
     2281 cccagccgcc gacgttcacc atcggctcca cggcgatcac caggccgggc ttgagctcgg
     2341 ggccccggcc cggctccccg tagttgggga tctgggggtc ctcgtgcatg ttccgtccga
     2401 tcccgtgacc cacgtactcg cggaccacct ggaaccccgc tccctctgcc acctgctgga
     2461 ccgcggcgcc cacatcggag agccggccgc ccgggcggat ctgcgcgatg cccgcctcga
     2521 gcgcctcctc ggtcacccgg accagcttct cggcctccgg cgaggggggc tcgcccacga
     2581 acaccgtcac cgccgagtcc ccgtggaacc cctcccagat cgcgccgaag tccagcgaga
     2641 ggaggtcgcc ctcgcggagc acgcgccggg gcgaggggat gccgtggacc acctcgtcgt
     2701 tcaccgacgc gcagatcgag gcggggaacg gcggctgccc gtaccccttg aacgacggga
     2761 tcgcgtcgcg ctcgacgatg tgggcctcgg ccacggcgtc caggtcggcc gtggtggcgc
     2821 ccggccggac cgcctccagc acccgctcga tggtggaggc gacgatgcgg ccggccttcc
     2881 gcatccggtc caggtcctcc cgcgacttgt aatcgatcac ccgaagtcct cggtgagatc
     2941 ggcgatcgcc tcgaccgtcc ggtccgccac gacctcctcg cgcgcctcgg cgtcgatgtc
     3001 gcggagcagg ccccgctccc agaagtacag gaccagcggc tgggtctcgc ggtggtacac
     3061 ctcgagccgg cggcggatcg tcgcctcgtc gtcgtcgctc cggttgacca gctcgtggcc
     3121 gcacacgtcg caggtcccct ccacccgggg aggcttgaac tcgaggttgt agctgcgctt
     3181 gcagttcggg cacaccagcc gcgccatcag ccgcttcacg gccatctcgt cggagatctt
     3241 gatgttcagc acggcgtgga gcggacgccc cgcctggctc agcgcccgct cgagggcctg
     3301 ggcctggggg accgtccgcg ggaacccgtc gaggatgaag ccctcgcgcg cgtcggggcg
     3361 gtccagccgc tcgagcacca tctcggtggt gatgtcgtcc ggcacgtact cccctcggtc
     3421 cacgtactcc ttggcctgca ggccgagcgg ggtggcgttc gcgatctgct cccggaagat
     3481 gtcgccggtg gcgatggtgg acaggccgta gcgctcggcc agcctcctgg cctgggtccc
     3541 cttccccgcc cccggtggtc cgaggaggat gatccgcacg gtccctcccc tatctcagga
     3601 agccctcgta gtggcgcatg aggagctgcg actcgagctg tttcatggtc tccagcgcca
     3661 cgccgatggt gatcagcagc gacgtcccgc cgaacgggaa ctgctggatc acccagaacg
     3721 cgaacacgat cgagggcagg agcgcgatcg tggccaggaa cagcgagccc ggcagcgtga
     3781 tccgggacag gaccttgctc aggtagtcgg acgtcggggg accgggccgg atcccgggga
     3841 tgaacccccc gtacttgcgg aggttgtccg cggtgtccac cgggttgaac gtgatcgcgg
     3901 tgtagaagta cgcgaagaac accaccatga gcccgtagaa ggtcatgtag accatgctgc
     3961 gctggttggt caggtagtcg ttcacgaagt tctggaacca gacggcatgg tagacgctgg
     4021 ccagcagcgt ggggaagtac aggaccgagc tggcgaagat gatcgggatc acgcctgcct
     4081 ggttgacctt cagcgggatg taggtgctgc cgcccgccgt catccgccgg ccgaccacgc
     4141 gcttggcgta ctgcacgggg atccgccgct gcccctgctc gatgaagatc acggccacca
     4201 cgatggccat cccgatcagc agcaccaccg tgaacttcgt ggtgccgccg gacagcagga
     4261 tcgagcggcc ctcctgcggg agccggctga tcacgctggt gaagatcagg atggacatcc
     4321 cgttgccgat cccccgctgg gtgatcagct cgcccatcca catgatcgtc gccgtcccgg
     4381 ccgtcatcac caggacgatc aacgcgatgt tctgcgcggt gaactcgccg gcggggaaga
     4441 tgtcgacgcc caggccggtg gtggagctcg gcgagtggaa caggaacacc aggccggtgg
     4501 actgcaggag cgccagcacc accgtgacgt agcgggtcca ctgattgatc ttcttggtgc
     4561 cggactcgcc ctccttctgc cagcgctcga gcttggggat caccaccgtg aggagctgca
     4621 tgatgatcga ggccgtgatg tagggcatga tgcccagccc gaacacggcc atccgcgtga
     4681 gcgccccgcc ggagaacagg tcgatgaacg agaggaaccc ggtcgccccc tgctgctcgg
     4741 ccgcctgctg caggatgctg atgtccacca ccgggaccgg cacgtgcgaa cccagccggt
     4801 acagggcgat gacgagcagg gtgaacagga tcttgttccg gaggtccggg accctgaagg
     4861 cgttgacgaa ggcgcggagc acgagacccc tatcctaccc cggtcagccg atcagctcgg
     4921 cccggccccc ggcgcgctcg atcttggccc tcgcggcctc ggagaaggcg tgggcgcgga
     4981 ccgtgagcgc cgtcccgagc tcgccgcggg ccaggatctt caccttccgc cccttgccga
     5041 ccaggccgtg cgcgtgcagg gccgccgggt cgacctcgcc cccgtcgaac accttgccga
     5101 gggtctccac gttcaccacg gcgtactcga ggcggttcgg gttggtgaag cccttcagct
     5161 tgggcacgcg gcgctggagc ggcatctggc cgccctcgaa cccggtcttg gggttgtgcc
     5221 gggccaggta gcccttggtc ccccggccgg ccgtcttgcc ccggacgccc gcccggccgc
     5281 gaccgacgcg cttgcgctcc cgcttggcgc cctccgccgg tctcaggtgg tggagcttca
     5341 tgccttcgcc ttccccttcc gcgaccggac ccggcgctcc gcgccgtccc cggacacctc
     5401 ctccaccgtc accaggtgct tgaccttctc gatcatcccg cggatctcgg gacggtcctc
     5461 cttgaccacc gagtcgttga tccggtgcag cccgagccgg cggaccgtgg ccttctggtc
     5521 cttcgggcgg tcgatgacgc tgcggacctg cgtgacccta agctgcgggc tcatcgccgc
     5581 ccaccccgcg gtccggtccg cggaccgctc cgctgcggcc ctggggaggc cgcggcctgg
     5641 tcgccgtaga cctcctccag cttggcggcc ctggtggcct gctgccgctc ctgcgcggcc
     5701 gcgacggcct cgagcatggg cttgggcgcc acctggtgga cgtcgcggcc gcgcaggcgg
     5761 gcgacctcct ccgggcgctt gaggctcttc aggcccgcca tggccgcctt cacgatgttg
     5821 atcgcgttgg ccgagcccag ggacttgctg aggatgtcct tgatgccggc gcactcgacc
     5881 agcgcccgca ccggtccgcc cgcgatcacg ccggtccccg gcgccgccgg cttgagcagg
     5941 accgcgccgg ccccgtcctc gccgatcacc tggtgcggga tggtggtgcc catcatcggc
     6001 acctcgaaca tcttccgctt ggcctcctcg acccccttgt tgatcgccgc cgggacctcc
     6061 ttggccttgc cgtagcccag gcccacccgg ccggcgccgt cgcccaccac caccagcgcc
     6121 gcgaacgaga agcgccggcc gcccttgacg accttggcca cgcggttgat gccgatgacc
     6181 cgctcctcgt agatgctctt ctcgccctgc gagtcgtacc gcctcatcag atctcgagcc
     6241 ccttctccct cgcgccctcc gcgagggcgg ccacccggcc gtggtagagc cgcccgcccc
     6301 ggtcgaacac gacccggtcg atcccggcgg ccttcgcccg ctcggccagc agctcgccga
     6361 cggccttggc cgccccggtc ttgcccggac caccggcggc cggaagctct cggtccgagg
     6421 ccgcgatcag cgtgcgggcg gcgtcgtcgt cgatcagctg ggcgtagatg tgccggttgg
     6481 accggtagac ggccaggcgc ggccgcgtcg gggtcccggc gatcttccgg cgcacacgat
     6541 ggtgacgccg caggcgggcg ccgcgcttgg tcttcgcctc catcacgcga tcgccccctt
     6601 cgccgccttg ccggccttct tgcggacgta ctcgttctcg taccggatgc ccttgccctt
     6661 gtagggctcg ggcttgcgga tcttccggat gttggcggcg gtctcgccga cctgctgctt
     6721 gtcgttgccc cggaccacga tggacgtggg ggtgggcgtc tcgaactcga tcccctccgg
     6781 gcacgggacc accaccgggt gcgagtagcc gacctggacc tccaggtcct tgcccttcat
     6841 cgccgcgcgg taccccacgc ccacgatcgc caggcgcttc tcgaacccct tggtcacgcc
     6901 ctcgaccatg ttggcgatca gcgtgcgcgt caggccgtgg aggctgcggt ggaagccctc
     6961 gtcgctgggg cgctcgaccc ggagggtgga gtcctcgcgg acgatccgca tctcggcgtg
     7021 cacgcgctgg ctcagggtcc cccggggacc cctcaccgtc acgacggcgt cgtcgccgag
     7081 ggcgacgtcc accccgctcg ggatctcgat gggctctcgt ccgatcctgc tcatccgctc
     7141 ttcttcttcc ccccgtggct ccgcttcccc gtcaccacac gtacgccagg acctcgccgc
     7201 ccacgcccgc gcgcgccgcg tctcggtcgg tcatcactcc tcgggacgtg gacacgatcg
     7261 cgatcccgag gccgccgagc acccggggca gctccccgcg acgtacgtag acccggcgcc
     7321 cgggcttgct cacccgccgc agcccggtga tcgtgcgctc ccgcttgggc ccgtacttca
     7381 ggtggaccct gagcacccgc tcgatcccct cgccctcgtc ctcgaacccg acgatgaagc
     7441 cctcggcgtc caggatccga aggatcgccc ggttcatctt cgaggcgggc agcgggaggt
     7501 cgtccttgta cgccaggttg gcgttgcgga tccgggtcag catgtccccg atcgggtccg
     7561 tgacgtgcgt cgcgagcctc accatgacgc cttcgtcacc cccggcagct ccccggcgtg
     7621 cgcgagctcg cggaagcaga tccggcacag gagcagcttg cggtacacgg cgcgggatcg
     7681 gccgcaccgg cggcagcgcg tgtactcacg cacccggaac ttcgcctgcc gctcctgctt
     7741 gttgatcaac gccttcttcg ccataccgct ccccaggcgc taggactccg cctcgccggc
     7801 catcggggcc ccctggaagg ggaacccgag ggcgacaagc agcgccctgc cctcctcgtc
     7861 cgtccgtgcg gtcgtcacga ccgtgatgtc catcccccgg accttcacga ccttgtcgta
     7921 gtcgatctcg gggaagatca gctgctcggt cagcccgagc gtgtagttcc cgtgcccgtc
     7981 gaacgccgcc gggtccagcc cgcggaagtc ccggatccgg gggatcgcga ccgagaccag
     8041 ccggtcgacg aactcccaca tccggtcgcc ccgcagggtg accttgacgc cgatcgccat
     8101 cccctcgcgc accttgaacc cggcgatcga cttgcgggcc ttcgtgatca ccggccgctg
     8161 gcccgtgatc accgcgaggt cgtcgacggc cgcctccagc atacgaccgt ccttcagggc
     8221 gtcgcccacc cccatgttca ggacgacctt ctccagccgc gggacctgca aggggttgga
     8281 catgccgagc tcgccctgga ggcgcggccg cgcctcctcg cggaagcgga ccttcatccg
     8341 gggcacgggg ggcgcctggc tcacaggtcc tcaccgcact tgcgacagat ccgcaccttc
     8401 gagccgtcgg gctcgaagcg gcggccgacc cgcgtgggct ggccgcacga cctgcacacg
     8461 agctgcacgt tggacaggtc gatgaacagc tcggtgtcga tgatgccgcc ctgctggagc
     8521 tgcgagccgc tggccgagcg cttcccctgc gtccgctggt gcttcttggc catcgcggcg
     8581 ccctcgacga gcacccggcc ctcgcgcggg agcacgcgga tcacccggcc ggtgtggccg
     8641 cggctcttgc cgttcatcac ccggacctga tcgtccttct tgatgtccac gcctggcatc
     8701 tagagcacct ccggagcgag cgagatgatc ttcatgaacc gcttctcccg gagctcgcgc
     8761 gcgaccgggc cgaagatgcg ggtcccgcgc gggttccgct gctcgttgat cagcaccacc
     8821 gcgttgtcgt cgaaccggat gtaggagccg tcgggtcggc gccgctcctt ggcggtccgc
     8881 accaccacgg cccggacgac gtcgcccttc ttcacggcgc cgccgggcag ggcgtccttg
     8941 accgagccga cgatcacgtc gccgatgcgc gcgtaccgct tgtggctgcc gccgagcacg
     9001 cggatgcaca ggaccacctt ggcccccgtg ttgtccgcca ccttgcaacg cgtctcctgc
     9061 tggatcaccg ggtcctctct cgttcccgcc tatctggtcc gccctacctg gccccgccct
     9121 acttggcccg ctcgacgatc tcgacgatcc gccagcgctt ggtcttgctg agcggccgcg
     9181 tctccatcac gcgcaccgtg tcgcccacgt gcgcgtcgtt ggcctcgtca tgcgcggcca
     9241 gcttcgacga ccgcttcacg atcttcttgt agagcgggtg ccgcaccttg cggtcgatgc
     9301 ggaccagcac cgtcttgtcc atggcgtcgg acacgaccac gccggtccgg accttccgcc
     9361 gtccccgctc cggcttcgcc ggctgctcgt cgctcacgcc tgctcccgtt cctcgtcctc
     9421 gacggcctcg agctcctcca gctcccgctc gcgcaggatg gtgaggatgc gcgcggcctc
     9481 gtggcgcgcc tgcttgatcc gcatcgggtt gtcgagctgg ccggtggcga gctggaagcg
     9541 taggttgaac agctcctcct tgagcgactc cacgcgctcg agcaggtcct catcgcccag
     9601 ttcacggatc tcgcggatct tggccactac tcgacacccc ccatccgcga gatgaacttg
     9661 gtcttgatcg gcagcttgtg cgccgcccgg ctcatggcct cgcgcgcgag cggctcggag
     9721 accccggaca gctcgaacat cacccgcccg ggcttgacca cggcgaccca gccctccggg
     9781 ttgcccttgc ccgagcccat ccgcgtctcg gccggcttct tcgtgtacgg cttgtcgggg
     9841 aagatgttga tccacacctt cccgccgcgg cggatgtgcc gggtgatggc cacccgggcg
     9901 gcctcgatct gccggttggt gatccacgcc ggctggaggg ccacgagccc gtagtccccg
     9961 tagtggatct ccgtgccgcc cttggcgcgt ccgcgccgcc ggccccgatg gaccttgcgg
    10021 tgcttgacct tcttgggggc gagcatctag ccctcctcct cgggcttggt ctccggcttg
    10081 gtctccggct tggcctcggg cttggcctcg ggcttggcct cgggcttggc ctcctcggcg
    10141 gcgggttccg ccggctcggc ctcgtcggtg atcaacgcgc cggtggaggc gccgccgctc
    10201 gagacctgcg cgagggcccg ggcccgcagg cgctcggtct ccagctcggc ctgcgggatc
    10261 tc
//
LOCUS       DASQQQ010000072        10916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_143649, whole genome shotgun sequence.
ACCESSION   DASQQQ010000072 DASQQQ010000000
VERSION     DASQQQ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10916
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143649"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(389..814)
                     /locus_tag="VE646_03135"
     CDS             complement(389..814)
                     /locus_tag="VE646_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZD79023.1"
                     /translation="MTLEHDLVLAELGRVPLFSGLDDDGMDRVARVVSEAEAPAGHVL
                     VQPAQPGTGMFVLQEGTAKVELPDRDIELGPGEFFGELAILADGIPRTARVQAVSAVR
                     CLAISRRDFAALLDECPQIAVAMLPVLAQRLADTDRAHH"
     gene            complement(811..1752)
                     /locus_tag="VE646_03140"
     CDS             complement(811..1752)
                     /locus_tag="VE646_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZD79024.1"
                     /translation="MSRGLRLLALSSALAVAGLSTSTAWAQEPEAPPAGPDLIVLSGT
                     ARVPRGSTVGEVVVLRGRAVIEGVAVGDVIVLDGPITVNGQVSGSVIAVTGVVRLGPG
                     AQVGGEVRARGTVRTAAGAHVAGGIRQRTAFTWRGPVEVFGRFAGWLAVTVSTFLLGL
                     LLVLMVPRALDTMSATARAAPWWSSGWGLALAIALPIAVLLLAVSLVGLPLALALLSG
                     FGLLGLLGYVIVMYLVGRLLWPSPRSRAVAFVFGWLILRAVGAVPYLSGATAGLAAVF
                     GLGVGAVALWRARSVGGKHREGKAVVWTEAMREEAGL"
     gene            1823..6217
                     /locus_tag="VE646_03145"
     CDS             1823..6217
                     /locus_tag="VE646_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZD79025.1"
                     /translation="MTLELFSPPVRDWFEASFEAPTAAQVRGWPAIASGGHTLVLAPT
                     GSGKTLAAFLWALDRLMSEPAPPPAERCRVLYVSPLRALAVDVEKNLRAPLAGIGLAA
                     ERLGLEAHAPTVAIRTGDTPASERRSIARRPPDILITTPESLYLMLTSQAREILRTIR
                     WVIVDEIHAMAATKRGAHLSLSLERLEAITRQPPQRIGLSATQRPLDEIARFLGGQAE
                     EGPRPVTIVDAGHRKPMEVEVIVPLEDMGQIGTMADTVPSGPAAAGPERISIWPSVHP
                     RLLQLIEQHRSTLIFANARRLAERLAARLNELAGEELVRAHHGSIAREQRLEIEDRLK
                     QGSLRALVATSSLELGIDMGAVDLVIQVESPGSVARGLQRIGRAGHSVGEPSRGKVFP
                     KYRGDLLEAAVVVERMRRGAIEETRYPRNPLDVLAQQIVAMCAVDEWTVSDLAALVRR
                     SATFADLSDELLRAVLDLMSGKYPSDDFAELRPRLVWDRRRDVLRAREGSGRLAVTSG
                     GTIPDRGLFGVFLPDGTRVGELDEEMVYESRRGEVFLLGASAWRIEDITLDRVVVSPA
                     PGEPGKMPFWKGDKPGRPLELGRAIGAFTRTMSALSPDEAVQRLQTGFGLDGWAAANL
                     VEYLHEQRDAAGAIPDDRTVVIERFRDELGDWRVCILSPFGAKVHAPWAMAVEARLSE
                     RLGMGAQVLWSDDGIVIRLPEAEERIPLEDLVFDREEIRDLVVRQLPATALFASVFRE
                     AAARALLLPRRGPTKRTPLWQQRQRSADLLQAAARHPDFPILLEATRECIRDVFDLPA
                     LEQVMGDLRARRILMVPVETPRASPFAQSLLFGWIAVYMYEGDAPLAERRAAALALDR
                     DLLHDLLGAEELRELLDPGVLDELELELQGLAERRRARNVDDLHDLLRLVGDLTAEEI
                     GSRSRGDPSRWIERLLHERQAFHVRVAGEERIAAVEDAGRLRDALGASVPQGIPAVFA
                     ETVAEPLRLLVARYARTHGPFHAADVARRLGIPLERVTGALVALESQGEVVLDEFRPG
                     GLEREWCDADVLRTLRRRSLAVLRREVEPVEAATLARFMPEWQGIGRPHRGPGALGEA
                     VEQLQGAALPASVLEHDVLPARVAGYRPSDLDALCAAGEVVWIGAGPLGADDGRVALF
                     FRDRASRLAPPPADEAPDGPVHRALRHHLANRGASFWPDLVRAAGTAEEAVVLTALWD
                     LVWAGEVTNDTLAPLRAFLLRRPSRARQAKPRPGAVRRTGPAAGQGRWSLVEPLLRPV
                     PSPTEIAHARAVQLLERHGVVTREAVLAEGTPGGFASVYGVLKSMEEAGRVRRGYFVA
                     GRGAAQFALPGAVDRLRQLREVGDRSGPAAVVLAATDPAQPYGAALPWPEGPGRPGRT
                     AGAFVVLVDGRLRGYLERGARSLVTFGDAADEPGWIDGLVALVKDGRLRRIELHRVDG
                     VPAHESLWATPLREAGFTDGYRGLVVRG"
     gene            6261..7067
                     /locus_tag="VE646_03150"
     CDS             6261..7067
                     /locus_tag="VE646_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HZD79026.1"
                     /translation="MPEGDTITRTARTLDRALAGRRLVRFQAPRLPAATLRPSAGSIV
                     LSARARGKHLLIDFSDGSTLHTHMRMHGSWHVYQPGERWRLPPAAMRAMVQVEGAVAV
                     CFRAPVVEVLEGGALRRHPQLTSLGPDLCAPAPDLDAAVEGLGRLLPGSTQIGVALLD
                     QRAAAGIGNVYKSEVLFACGIDPFTPVTALDEATRRRVVETAARLLRANLDARAPRRT
                     VPEGLAVYGRAGRPCRRCGTPIRSRKQGEAARATYWCPACQPAIVPEPAP"
     gene            7064..8662
                     /locus_tag="VE646_03155"
     CDS             7064..8662
                     /locus_tag="VE646_03155"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZD79027.1"
                     /translation="MSARVLSATGSAAAIATPHRAATDAGAAAFADGGNAIDAALAAA
                     VTLAVVYPQQCGVGGDLFALVAEPGGAVNAVNASGPAPRALDPRQVRAMHRAMPEFGP
                     LSVTVPGAVAGWSALHGLGAALPWTELFAPAVGWAREGVSTVRSMAARLEAAGERLSS
                     DPGMAQVFYPRGRPLVEGEPFVQPVLADTLEAIAEAGAAAMYGGAMGDRLAAGLQALG
                     SPMSSEDLASFSPERTPPLTSPYRGLEVAVVPPNSPGFALLEMLAVVERLGIDPDPLG
                     PDAALLAAVFEAASADRDRHNADPRVAAVPVDELLADEHLDRLCHAARGATGPSSAGP
                     ATPARHRDGGDTVALVAADGEGRLVSLVQSLWSSFGSGLLEPSTGIILHNRGNDFTLD
                     PSSPNVLAGGKRPAHTLTPVLVRREGRPVGASGSRGGGGQPQINYMNLVRAFDLGMDA
                     AAAVAAPRYLVGGMDLHAERFADVEARVPNQVRDALASSGFRVDLLADLDDAVGHAHL
                     VLRDADGSLDIGTDPRADGTAWAR"
     gene            complement(8676..>10916)
                     /locus_tag="VE646_03160"
     CDS             complement(8676..>10916)
                     /locus_tag="VE646_03160"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZD79028.1"
                     /translation="AAELAWAATTVVGIVPMSVSVVRDLLHRETGVDIIALLAMVGAL
                     AFGEYLAGAVIALMLSSGQALESYADRRAHRELSALLERAPRSVHRYTDRGLESVPIE
                     RVRPGELLMVKKGEVVPVDGFLASEHAVVDESALTGEARPVEREEGDQLRSGSVNSGG
                     SFDLRATATAEESTYAGIVRLVEEAQTQKAPFVRLADRYALIFLPLTLVMAGGAWIWT
                     GDPVRALAVLVVATPCPLILAAPIAIVAGISRAAKRGIIVKGGGALETLARGSVLLFD
                     KTGTLTAGVPELADVEVFSDVDPDELIRLAASLDQVSPHVLAAAIVRAARDRGATLSF
                     PEGVHEEHGAGIEGKVDARLVSLGKTSWVTRGGTLPPRARDVRRRSAMEGSSCVFVAV
                     DGRVAGALILDDPIRPDTPRVIRTLRRAGIQRVVMVTGDHADVAESVGISIGVDRILS
                     ERDPADKVDAVTAEGDEGVTIMVGDGVNDAPALAAANVGVAMGARGATASSEAADVVL
                     VVDRLDRLAEAMRIARRSRGIAVQSVLIGMGLSFAGMVLGAVGLLRPVAGALVQEAID
                     VAVILNALRALGGDREARRRPRLTSNVGDRFREEHRDFMPWLARIRTAADRLDTYAPA
                     EARDELEQIRWFVTERLVRHEEEEEAAVYPVVSKLMGGEDPMGTMARAHLEIEHLARV
                     FVHLLEGLLPEGPGPDDLVDLRRVMYGLHAILRLHFAQEEEAYAWLASDEPDELAHRH
                     EASPVG"
BASE COUNT         1427 a         4093 c         3970 g         1426 t
ORIGIN      
        1 ggtcctgatg gggctgtcgc aggcggcggc gttcggggcc gcgctgggcg tggtggtcgg
       61 ggccatcctc cgcttccgcc gcggcgagga ctgaacgtca tgaagagggg tctccccggt
      121 ggcccccctg cggcgcccac cgaaggagcg ccgggaggag gccccggcca ggttgcacgc
      181 cggtttggac ctgtccaggc gcagcctgga cgagcatctg ctcgagctcg cgggctgggt
      241 gctcgagatc gccgatgcca gaagggcaag gacccggcgc cgctcgcccg caggaccggc
      301 cggatcgacg cgtgggggct tgctcgcccg tcgggatcca ccccccgcgc cggggtgccc
      361 ggcctcacga ccgcctcgga actcggcgct agtggtgggc ccggtcggtg tcggccagcc
      421 gctgggccag gaccggcagc atcgccacgg caatctgcgg acactcatcc aacagcgcgg
      481 cgaagtcccg gcgtgagatg gccaggcatc ggacggcgct caccgcttgg acccgcgcgg
      541 tgcgggggat cccgtcggcc aagatcgcga gctccccgaa gaactcgccc ggtccgagct
      601 cgatgtcccg gtccggcagc tccaccttcg cggtgccctc ctggaggacg aacatgcccg
      661 tgccgggctg ggcgggttgc acgaggacgt ggccggcggg cgcctcggcc tccgagacca
      721 cacgagccac ccgatccatg ccgtcgtcgt ccaggcccga gaacaggggc accctcccca
      781 gctccgccag caccagatcg tgctccaagg tcatagccct gcctcctccc gcatggcctc
      841 cgtccagact acggccttcc cctcccggtg cttgcccccc accgaccgtg cccgccagag
      901 cgccaccgcg ccgaccccga ggccgaacac cgccgccaga ccagccgtgg cgccgctgag
      961 gtaggggacg gcgcccacgg cccgcaggat cagccatccg aacacgaacg ccaccgcccg
     1021 gctccggggc gatggccaga gcagccgtcc cacgaggtac atcacgatca cgtagccgag
     1081 caggccgaga aggccgaagc ccgacaggag ggccagggcg agcggcagcc cgaccaacga
     1141 tacggcgagc agcagcacgg cgatgggcag ggcgatcgcc agggctaggc cccagccgga
     1201 cgaccaccag ggcgccgccc gagccgtggc ggacatcgta tccagggccc gaggcaccat
     1261 caggaccagc aggaggccca gcaagaaagt cgagacggtc accgccagcc atccggcgaa
     1321 ccgtccgaag acctccacgg gtccccgcca cgtgaaggcg gtccgctgcc gaatcccccc
     1381 cgccacgtgg gcgcctgcgg cggtccgaac cgtccctcgg gcccgcacct cgccgcccac
     1441 ctgggccccc ggacccagcc tgacgacgcc ggtgaccgcg atcacgctcc cgctcacctg
     1501 gccgttcacg gtgatcggtc cgtccaggac gatcacgtcg cccacggcca ccccttcgat
     1561 caccgcgcgc ccgcggagca cgacgacctc ccccaccgtc gagcccctgg gcacccgggc
     1621 ggtgccggac aggacgatca ggtcggggcc cgccggtggg gcctcgggtt cctgcgccca
     1681 ggcggtggat gtgctcagcc cggccaccgc gagcgccgac gataaggcga gcagccggag
     1741 gcccctcgac atggcgagca tcctaccggc gcccgcatcc gggtcgagga cccactgtcg
     1801 ggtcccgcgg tagcgtgtgc gggtgacgct ggaactgttc tcccccccgg tccgggattg
     1861 gttcgaggcc tctttcgagg cgcccacggc ggcgcaggtc cgcggctggc ccgccatcgc
     1921 ctcgggcggt cacacgctcg tcctggctcc gaccggatcg ggcaagacgc tcgcggcgtt
     1981 cctgtgggcg ctcgaccgcc tgatgtccga gccggcgccc ccaccggccg agcgatgtcg
     2041 tgtgctgtac gtgtcgccgc tccgagcgct ggcggtggac gtggagaaga acctgcgggc
     2101 gccgctggcc ggcatcgggc tcgctgccga gcgactgggg ctcgaggcgc acgcacctac
     2161 ggtggccatc cggaccggcg acacgccggc gagcgagcgg agatccatcg cccggcgccc
     2221 ccccgacatc ctgatcacga cgcccgagtc cctgtacctc atgctcacct cgcaggcccg
     2281 cgagatcctg cggacgatcc gctgggtgat cgtggacgag atccacgcga tggcggccac
     2341 caagcgtgga gcccacctct cgctgtcgct cgagcggctg gaggccatca cccgacaacc
     2401 gccgcagcgg atcggcctgt cggccacgca gcgccccctg gacgagatcg cccggttcct
     2461 gggcggccag gcggaggagg ggcccaggcc ggtgacgatc gtggacgccg gccaccgcaa
     2521 gcccatggag gtcgaggtga tcgtcccgct ggaggacatg ggccagatcg gcacgatggc
     2581 cgacacggtc cccagcgggc cggcggcagc cggacccgag cggatcagca tctggccctc
     2641 cgtccatcct cggctgttgc agctgatcga gcagcaccgc tccacgctga tcttcgccaa
     2701 cgctcgccgg ctggcggagc ggctggccgc ccgcctgaac gagctggccg gcgaggagct
     2761 ggtccgcgcg caccacggct cgatcgctcg ggagcagcgg ctggagatcg aggaccggct
     2821 caagcaggga tcgctccggg cgctggtggc caccagctcg ctcgagctcg gcatcgacat
     2881 gggggcggtc gacctggtga tccaggtgga gtcgccgggc tcggtggccc gggggctgca
     2941 gcggatcggc cgggccggac actcggtggg cgagccctcc cggggcaagg tgttcccgaa
     3001 gtaccggggc gacctgttgg aggccgcggt ggtggtggag cggatgcggc ggggcgcgat
     3061 cgaggagacc cgctaccccc ggaacccgct ggacgtgctg gcgcagcaga tcgtggcgat
     3121 gtgcgcggtg gacgaatgga ccgtgtccga cctcgccgcg ctggtgcggc gctcggcgac
     3181 cttcgcggac ctgtccgacg agctgctgcg cgccgtgctg gacctgatgt ccgggaagta
     3241 cccgagcgac gatttcgccg agctccgtcc ccggctggtg tgggaccggc gtcgtgacgt
     3301 cctgcgggcc cgggagggat cggggcggct ggccgtgacc agcgggggca cgatccccga
     3361 ccgcggcctg ttcggcgtgt tcctgccgga cggcacccgt gtgggcgagc tcgacgagga
     3421 gatggtctac gagagccgcc ggggggaggt cttcctgctc ggggcatcgg cgtggcggat
     3481 cgaggacatc accctggacc gggtggtggt ctcgccggcc cccggcgagc cggggaagat
     3541 gcccttctgg aagggggaca agcccggacg gcccttggag ctagggcggg cgatcggcgc
     3601 gttcacccgg accatgagcg ccctgtcccc cgacgaggcg gtccagcggc tccagaccgg
     3661 gttcggcctg gacgggtggg ccgcggccaa cctggtcgag tacctgcacg agcaacgcga
     3721 cgcggccggg gccatccccg acgaccggac cgtggtgatc gagcgcttcc gggacgagct
     3781 cggcgattgg cgcgtgtgca tcctgtcccc gttcggggcc aaggtccacg cgccctgggc
     3841 catggccgtc gaggcccgtc tgagcgagcg cctgggcatg ggcgcccagg tgctgtggag
     3901 cgacgacggg atcgtgatcc gcctccccga ggccgaggaa cgcatcccgc tcgaggacct
     3961 cgtgttcgac cgggaggaga tccgagacct ggtggtccgc cagctgcctg ccaccgcgtt
     4021 gttcgcctcg gtcttccgcg aggcggccgc ccgcgccctc ctcctgcccc gtcgaggccc
     4081 caccaagcgc accccgctgt ggcagcagag gcagcgctcc gcggacctgc tgcaggccgc
     4141 cgccaggcat ccggacttcc cgatcctgct cgaggccacc agggagtgca tccgcgacgt
     4201 gttcgacctg ccggccctcg agcaggtgat gggcgacctg cgggcccgga ggatcctgat
     4261 ggtgcccgtg gagacgccgc gggcgtcccc cttcgcgcag tccctgctgt tcggctggat
     4321 cgccgtgtac atgtacgagg gcgatgcgcc cctcgccgag cgtcgggccg ccgcgctcgc
     4381 cctcgaccgc gacctcctgc acgacctgct gggcgccgag gagctccgcg agctcctgga
     4441 ccccggggtc ctggacgagc tggagctcga gctccagggc ctggccgagc gccggagggc
     4501 ccggaacgtc gacgacctcc acgacctact gcgcctggtg ggcgacctga ccgctgagga
     4561 gatcggcagc cgcagccgcg gcgatcccag ccgttggatc gagcgcctgc tccacgagcg
     4621 gcaggcgttc cacgtgcggg tggccggcga ggagcggatc gcggccgtgg aggacgccgg
     4681 gcgcctgcgc gacgccctgg gcgcgagcgt gccgcagggc atcccggcgg tcttcgcgga
     4741 gaccgtggcc gagcccctgc ggctgctggt ggcccgctac gcgcgcacgc acgggccgtt
     4801 ccacgcggcg gacgtggccc ggcgtctggg gatcccgctc gaacgggtca ccggcgctct
     4861 ggtcgcgctc gagtcgcagg gggaggtggt gctcgatgag ttccggcccg gcggcctgga
     4921 gcgcgagtgg tgcgacgcgg acgtcctgcg cacgctgcgg cgccggtcac tggccgtgct
     4981 ccgccgggag gtcgagcccg tcgaggcggc gaccctcgcg cggttcatgc ccgagtggca
     5041 gggcatcggg cgtccccacc ggggcccggg agccctgggc gaggcggtcg aacagctcca
     5101 gggggcggcc ctcccggcgt ccgtcctgga gcacgacgtc ctgccggccc gcgtggccgg
     5161 ctaccgcccc tcggacctgg atgcattgtg cgcggcgggg gaggtcgtct ggatcggggc
     5221 tggtccgctc ggcgcggacg acggcagggt ggccctgttc ttccgcgatc gtgcgtcccg
     5281 gctggccccg ccaccggccg atgaggcgcc cgatggaccg gtccatcggg ccctccgcca
     5341 ccacctcgcc aaccgagggg cctcgttctg gccggacctt gttcgggccg ccgggacggc
     5401 cgaggaggcg gtcgtcctga cggccttgtg ggacctggtc tgggccggcg aggtcacgaa
     5461 cgacaccctc gcaccgctcc gtgcgttcct gctccgccga ccgtcccggg cccggcaggc
     5521 caagccgcga ccgggtgccg tgcgccgcac cggtccggcg gccgggcagg gacggtggtc
     5581 gctggtcgag ccgctcctgc gtccggtccc ctcccccacc gagatcgccc acgctcgggc
     5641 cgtccagctg ctcgagcgcc acggcgtggt cacccgggag gccgttctcg ccgagggcac
     5701 acccgggggg ttcgcgagcg tgtacggggt gctgaagtcg atggaggagg cgggacgggt
     5761 ccgccgcggt tacttcgtgg ccgggcgggg cgctgcccag ttcgccctgc ccggggccgt
     5821 ggaccggttg cggcagctcc gggaggtggg tgatcggtcc ggccccgctg cggtggtgct
     5881 ggcggccacg gacccggccc agccctacgg cgccgccctg ccttggccgg agggtccggg
     5941 tcgccccggc cgaaccgcgg gggcgttcgt ggtgctcgtg gacggtcggc tgcgggggta
     6001 cctggagcga ggcgcgagga gcctggtcac cttcggggac gccgccgatg agcccggttg
     6061 gatcgacggg ctcgtggccc tggtgaagga cgggcggctg cgcaggatcg agctccaccg
     6121 ggtggacggg gtcccggcgc acgagtccct ctgggccacc ccgctccgtg aggccggctt
     6181 caccgacggc taccggggcc tggtcgtccg cggttgagcc agcggccctc cccatcgtcc
     6241 gcgacgccta tccttgggcc atgcccgagg gcgacacgat caccaggacc gcgcgcaccc
     6301 tcgatcgcgc cctggccggc agacggctgg tccggttcca ggctccacgg ctccccgccg
     6361 ccaccctccg gccctccgcg ggctcgatcg tcctgtcggc tcgcgcccga ggcaagcacc
     6421 tcctgatcga cttctcggat ggatccacgc tccacacgca tatgcgcatg cacgggagtt
     6481 ggcacgtgta ccagcccgga gagcggtggc gcctgccgcc cgccgccatg cgggcgatgg
     6541 tccaggtgga gggtgcggtc gccgtgtgct tccgagcgcc agtggtggag gtgctcgagg
     6601 gcggggcgct ccgccggcac ccccagctca cgtccctggg accggatctg tgcgcgcccg
     6661 cgcccgacct ggatgcggcg gtggaagggc tgggccgcct gctccccggc tcgacgcaga
     6721 tcggagtggc cctcctggac cagcgcgcgg cggccgggat cggaaacgtc tacaagagcg
     6781 aggtgctgtt cgcctgtggc atcgacccct tcacgccggt gaccgccctg gacgaggcga
     6841 cccgccggcg cgtggtggag acggccgcga ggctcctgcg cgcgaacctg gacgctcgcg
     6901 ccccgcggcg caccgtaccc gagggcctgg ccgtctacgg ccgtgccggg cgaccctgcc
     6961 gtcgttgtgg caccccgatc cgctcgagga agcagggcga ggcggcccgg gcaacgtact
     7021 ggtgtcccgc ctgtcagccg gcgatcgtcc cggagcccgc tccgtgagcg cccgggtcct
     7081 gtccgcgacc ggctcggcag ccgccatcgc cacgccgcac cgcgccgcga ccgacgccgg
     7141 agcggccgcc ttcgcggacg gcggcaacgc gatcgatgcc gccctggcgg ccgcggtcac
     7201 gctcgccgtc gtctacccgc agcagtgcgg cgtgggtggg gacctgttcg cgctggtagc
     7261 cgagccgggc ggggccgtca acgcggtgaa cgccagcggc cctgcgcccc gcgcgttgga
     7321 tccgcgacag gtgcgagcca tgcaccgagc gatgcccgag ttcggtccgc tctcagtgac
     7381 ggtgcccggc gccgtcgccg gctggtcggc cctgcacggc ctgggggccg cgctcccctg
     7441 gacggaactc ttcgctccgg ccgtgggctg ggcgcgggag ggtgtgtcga ccgtgcggtc
     7501 catggccgcg cgcctagagg ctgcgggcga gcggctgagc tccgatccgg ggatggccca
     7561 ggtcttctat ccgcgaggtc gcccgctcgt cgagggcgag cccttcgtgc agccggtgct
     7621 cgcggacacg ctcgaggcga tcgcggaggc cggcgcggcc gcgatgtacg gcggcgcgat
     7681 gggcgatcga ctggccgccg ggctgcaggc cctgggctcg ccgatgtcgt ccgaggacct
     7741 ggcgtccttc tcgcccgagc ggacgcctcc cctcacgagc ccgtaccggg ggctcgaggt
     7801 ggccgtggtt ccacccaact caccgggctt cgcgctcctc gagatgctcg ccgtggtgga
     7861 gcggctgggg atcgaccccg atcccctggg gccggacgcg gcgctcctgg ccgcggtgtt
     7921 cgaagcggca agcgcggatc gggaccgcca caacgccgat ccgcgggtgg ccgcggtccc
     7981 ggtcgacgag ctactcgccg acgagcacct cgaccggctc tgccacgccg cccgcggcgc
     8041 caccggcccc agctcggccg ggccggccac accggcccgc catcgggacg gcggcgacac
     8101 cgtcgccctg gtcgccgccg acggcgaggg ccgactggtg tcgctggtcc agagcctgtg
     8161 gagctcgttc ggctccggcc tcctggagcc ctccacgggg atcatcctgc acaaccgcgg
     8221 caacgacttc acgctcgatc cgagctcccc gaacgtcctg gccggcggga agcgtcccgc
     8281 ccacacgctg acgccggtcc tcgttcgtcg ggagggacga ccggtcggcg ccagcggatc
     8341 gaggggcggg ggcggtcaac cgcagatcaa ctacatgaac ctggtccgtg ccttcgacct
     8401 ggggatggat gcggcggccg cggtggcggc gccccgctac ctggtgggtg ggatggacct
     8461 gcacgccgag cggttcgcgg acgtcgaggc tcgggtcccg aaccaggtcc gggacgcgct
     8521 ggcctcgtcc gggttcaggg tcgacctgct cgccgatctg gacgacgcgg tggggcacgc
     8581 acacctcgtg ctccgggacg cggacggctc cctcgacatc ggcaccgatc cccgggccga
     8641 cggcaccgcc tgggcgcgct gacgcggcgc gggcgtcagc cgaccggact cgcctcgtgc
     8701 cggtgcgcga gctcgtccgg ctcgtcggag gccagccagg cgtacgcctc ctcctcctgc
     8761 gcgaagtgca gccggaggat cgcgtgcagg ccgtacatca cccggcgcag gtcgaccagg
     8821 tcgtcggggc cgggaccctc gggaaggagc ccctccagca ggtgcacgaa cacccgggcc
     8881 aggtgctcga tctccaggtg cgcccgcgcc atcgtgccca tggggtcctc gccgcccatg
     8941 agcttggaga ccacgggata gaccgccgcc tcttcctcct cctcgtgccg gaccagccgc
     9001 tcggtgacga accaccggat ctgctcgagc tcatcgcggg cttcggccgg agcgtaggtg
     9061 tccaggcggt cggccgcggt gcggatccga gccagccacg gcatgaagtc ccggtgctcc
     9121 tcgcggaacc gatcgcccac gttcgaggtc aggcgcgggc ggcgccgggc ctcccggtcg
     9181 ccccccagcg cccgcagggc attgaggatc accgccacgt cgatcgcctc ctggaccagc
     9241 gccccggcca ccgggcggag cagcccgacc gcccccagga ccatgccggc gaacgacagc
     9301 cccatgccga tcagcacgct ctgcacggcg atcccgcggg accggcgcgc gatgcgcatg
     9361 gcctcggcca gacggtccag gcggtccacc accagcacca catcggccgc ctccgaggag
     9421 gccgtcgctc cccgggcgcc catcgccacg ccgacgttcg cggcggccag cgccggcgcg
     9481 tcgttcacgc cgtcgccgac catgatagtc acgccctcat cgccctcggc ggtcaccgca
     9541 tccaccttgt cggccgggtc gcgctcggac aggatccggt ccacgccgat cgagatcccc
     9601 accgactcag ccacgtcggc gtggtcgccg gtcaccatca ccacccgctg gatcccggcc
     9661 cgtcgcagcg tccggatcac gcggggcgtg tcggggcgga tcgggtcatc gaggatcagt
     9721 gccccggcca cccggccgtc aacggctacg aacacgcagc tggagccctc catcgccgag
     9781 cgccgccgca cgtcgcgggc ccggggcggg agcgtccccc cgcgggtcac ccacgaggtc
     9841 ttccccagag agaccagccg ggcgtccacc ttgccctcga tcccggcgcc gtgctcctcg
     9901 tgcacgccct cggggaagga cagggtcgcc ccgcggtcgc gtgcggcgcg gacgatggcc
     9961 gccgccagca cgtggggaga cacctggtcc agggaggcgg ccagccggat cagctcgtcg
    10021 ggatcgacgt cggagaacac ctcgacgtcg gcgagctcgg gcacgcccgc cgtcagggtc
    10081 cccgtcttgt cgaacaagag gaccgagccg cgagccaacg tctccagcgc gcccccgccc
    10141 ttgacgatga tcccgcgctt ggcggcccgc gagatccccg ccacgatcgc gatcggcgcc
    10201 gccaggatca gcgggcacgg tgtggccacg accagcacgg ccagcgcccg caccggatcc
    10261 cccgtccaga tccacgcgcc cccggccatc accagggtca ggggcaggaa gatcagcgcg
    10321 taccggtccg cgagccggac gaacggggcc ttctgggtct gggcctcctc caccaggcgg
    10381 acgatccccg cgtaggtgct ctcctcggcc gtggccgtgg cccggaggtc gaacgacccg
    10441 cccgagttca cggatccgct gcgcagctga tcgccttcct cgcgctcgac cggccgggct
    10501 tcccccgtga gggcggactc gtccaccacg gcatgctcag aggctaggaa gccgtcgacc
    10561 ggcaccacct cgcccttctt gaccatgagg agctcacccg gccggacccg ctcgatcggc
    10621 acggactcca atcctcgatc ggtgtagcgg tggaccgagc gcggcgcccg ctccagcagg
    10681 gcggacagct cccggtgggc acgacggtcc gcgtaggact ccagagcctg cccgctggag
    10741 agcatgagcg cgatcaccgc gccggccagg tactcgccga acgccaacgc gccgaccatg
    10801 gccagcagcg cgatgatgtc gacccccgtc tcccggtgga gcaggtcgcg gaccaccgac
    10861 acggacatgg gcacgatccc caccacggtg gtggcggccc atgccagctc ggccgc
//
LOCUS       DASQQQ010000073         4952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_60955, whole genome shotgun sequence.
ACCESSION   DASQQQ010000073 DASQQQ010000000
VERSION     DASQQQ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60955"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1390)
                     /locus_tag="VE646_03165"
     CDS             complement(<1..1390)
                     /locus_tag="VE646_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HZD79029.1"
                     /translation="MTIDTGSLSTPSIERPVFLPRSGMGGLTRRMAVASALLAGVIVA
                     AFTMLFLAVVNFTDSTDESRHTRQELVVADQLDKLILDLETGLRGYVITGEERFLAPW
                     NDARAAFPRQAAELERLVAGDPGQVTHARELVRMGESYIQDYGVPVIQAVRRGDPSAH
                     SVAVTEDGKRRMDALRAEFDRFAVIGRADLNQREASAGEAGRRAVIGAEVGVGSSILL
                     ILLFTGYLTRVIVRPIRAAAFMANRLATGDLSTRIPERGVGEIGTLERSFNFMADSLE
                     QDRGELSRLAEEQGALRRVATLVARAVPPADVFSAVTREVAALLGADTAYLARYQPNG
                     TVTVVARWSGEGTGAAGLGSTAENHAVTDRVRETRRSARLDHPAEVELGGSAPVTGAH
                     HRSWVAAPITVGGRLWGAMVASSVRPDPLPEDAEARMTNFTDLVGTAIANSQARTDLA
                     ASRARVVSAADES"
     gene            complement(1387..1797)
                     /locus_tag="VE646_03170"
     CDS             complement(1387..1797)
                     /locus_tag="VE646_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011595330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZD79030.1"
                     /translation="MSLRALVVDDNEGFTDVARGVLEREGIIVVGVAKNSDEAVRLAQ
                     DLYPDVVLVDIDLGEESGFDLVHRLGAEADVGANAILISTHAEEDFADLIAESPALGF
                     LAKSRLSAGAILDMIRGTDAGGMDPETGGEPPGP"
     gene            complement(1910..2323)
                     /locus_tag="VE646_03175"
     CDS             complement(1910..2323)
                     /locus_tag="VE646_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZD79031.1"
                     /translation="MGAQLGLRWIDGALHVEGELDLATAEGLLTEVERARPSGELVLD
                     LSHLCFIDSEGCRAIARLTGRTNGRVSLVVLRDPRPPVRRVFDLLRLSGRAIRIMDAE
                     QGLHQPPDPQGPVHGSVSGSDGVGGSPFPLTGRCR"
     gene            complement(2498..3151)
                     /locus_tag="VE646_03180"
     CDS             complement(2498..3151)
                     /locus_tag="VE646_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD79032.1"
                     /translation="MRPARVVLADDDVLLREGLATVFERAGSRVVGRAGTASELLSLV
                     RAEHPDLAIVDIRMPPGHSTEGLDAARLIRQDFPEVAIMVLSAHVEVEHAMELLASGD
                     RLGYTLKSRVTDVQSFLASVEQILDGGTVIDPEVVQELVGARRSHDPLETLTDREREV
                     LALMAEGRSNAGIAARLWLAEGTVEKHVRSILAKLALAEAPDDHRRVLAVLRYLESR"
     gene            complement(3252..3325)
                     /locus_tag="VE646_03185"
     tRNA            complement(3252..3325)
                     /locus_tag="VE646_03185"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3289..3291),aa:Met,seq:cat)
     gene            3428..3973
                     /locus_tag="VE646_03190"
     CDS             3428..3973
                     /locus_tag="VE646_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79033.1"
                     /translation="MRVKPILAVAAVALVAAACSSSGPTTAPTASPSAASPSVSPAAT
                     PSEGASPSAQSPAAAKAEIERNWTRFFNGQTPPGQKIDLLVDGQQFAGVIEASSQSQL
                     ARSTRAKVTNVSLTGPDTATVTYDVLVGGQPALTDQQGTAVRTDGTWRVSDASFCSLL
                     ALGGQVPAACPSPGASPSASG"
     gene            3970..>4952
                     /locus_tag="VE646_03195"
     CDS             3970..>4952
                     /locus_tag="VE646_03195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD79034.1"
                     /translation="MRAPAGRGRGRRLATLATILFLTFLDNTIVSVILADMQTTLHPG
                     VSGLQWIVNGYMLAFAALMLTGGTLGDLLGRKKVMLSGVAVFCAGSVAAALAPTTSVL
                     IGSRVVMGIGAAASEPGTLSMIRHIYPERGPRARALGVWAAVSGVALALGPIVGGVLV
                     GYSGWRSVFWFNLGFGAAALAAGVLLLPENADPEGRRLDVPGLIVGAVTISAIAIAVI
                     EGENAGYLSWWIDLLFAVAALGVVAFIRIERRSPDPVLKLHFFQTSAFSGANVVAFAT
                     NFGVFAVFFFTALYLQLVADFSGYRIALQFVTMAAAMVAAAPLAGRWVAARG"
BASE COUNT          710 a         1806 c         1673 g          763 t
ORIGIN      
        1 ggctctcgtc ggccgcggag accacccgcg ccctcgaggc cgcgaggtcg gtccgggcct
       61 gcgagttcgc gatggccgtc ccgaccaggt cggtgaagtt cgtcatccgg gcctccgcgt
      121 cctcgggcaa gggatcgggg cgcaccgaag aagcgaccat cgcgccccag agccgccctc
      181 cgacggtgat cggggcggcc acccacgagc gatggtgggc ccccgtgacg ggcgctgatc
      241 cgccaagctc gacctccgcg gggtggtcga gccgggcgct ccgacgtgtc tcccgcaccc
      301 gatccgtgac cgcgtggttc tccgcggtgc ttccgagccc ggcggctccg gtcccctccc
      361 cgctccaccg ggccacgacc gtcaccgtgc cgttgggctg gtaccgggcc aggtacgcgg
      421 tatcggctcc gagcaacgcg gccacctcac gggtcacggc ggagaagacg tcggcgggag
      481 ggacggctcg ggccaccagc gtcgccactc gtcgcagggc cccctgttcc tcggccagtc
      541 ggctgagctc cccccgatcc tgctccagcg agtcggccat gaagttgaac gagcgctcca
      601 gggtgccgat ctcccccacc ccccgctccg ggatccgggt gctgaggtcg ccggtggcca
      661 gtcggttcgc catgaacgcc gccgcccgga tcggccggac gatcacgcgc gtgaggtagc
      721 cggtgaacag caggatgagg aggatcgagc tccccacccc gacctcggcg ccgatgaccg
      781 cccgccgacc cgcctcgccg gcgctggcct cgcgctggtt caggtcggcc cgaccgatga
      841 ccgcgaaccg gtcgaactcg gcacgcagcg catccatcct ccgcttgcca tcctcggtca
      901 cggccacgct atgggccgac gggtctccgc ggcggaccgc ctggatcacc ggaacaccgt
      961 agtcctggat gtaggactca cccatacgga ccagctcgcg ggcatgggtc acctgccccg
     1021 ggtctcccgc caccaggcgt tccaactccg ccgcctgccg cgggaacgcg gcccgggcgt
     1081 cgttccaggg agctaggaac cgctcctccc cggtgatgac gtacccacgg aggcccgtct
     1141 ccaggtcgag gatcagcttg tccagctggt cggcgaccac gagctcctgt ctcgtgtggc
     1201 ggctctcgtc cgtggagtcg gtgaagttca ccacggccag gaacagcatg gtgaacgcgg
     1261 cgacgatcac gccggccagc agggcgctgg cgacagccat ccggcgggtc agcccgccca
     1321 tcccggatcg ggggagaaag acggggcgct cgatgctcgg cgtgctgagc gaaccggtgt
     1381 cgatcgtcat ggaccgggag gttctccgcc ggtctcgggg tccatccctc ccgcgtcggt
     1441 cccgcggatc atgtcgagga tcgcaccggc ggagagccgg gacttcgcca ggaagcccaa
     1501 cgccgggctc tccgcgatga ggtcggcgaa gtcctcctcg gcatgggtgg agatcaagat
     1561 ggcgttggcc ccaacatcgg cctcggcccc gagccggtgg accagatcga agccgctctc
     1621 ctcgcccagg tcgatatcca cgagcacgac gtccggatac aggtcctggg caagccgaac
     1681 cgcctcatcg ctgttcttgg ccacgccgac gacgatgatc ccctcccgct ccaagacgcc
     1741 acgggcgacg tcggtgaacc cctcgttgtc gtccacgacg agcgcccgca agctcatcac
     1801 gccagcatcc cagggatcgg ggccctcagg catcccagct agcttccctt cgtcctgagg
     1861 ctacctggtg ggctgctacc cctcggagcg tggcgatgca tcggacagcc taccggcagc
     1921 gccccgtgag ggggaagggg gaaccaccca ccccgtcgga gccggagacc gatccgtgga
     1981 cgggtccctg cgggtcaggg ggttggtgga gaccctgctc cgcgtccatg atccggatgg
     2041 ctcgcccgga caagcgaagg agatcgaaga cccgtcggac gggcgggcga ggatccctca
     2101 ggacgaccag cgagacgcgt ccgttcgtcc gtccggtcag gcgggcgatg gcccggcaac
     2161 cctccgaatc gatgaagcac aggtgtgaga ggtccaggac gagctctccg gacggacgtg
     2221 cccgctccac ctccgtcagt aggccctccg cggtcgccag gtccaattcg ccctccacgt
     2281 gcagtgcccc gtcgatccat cgaagcccga gctgtgctcc catcgtgcga ccgtcccttc
     2341 ctcagcgtcc cctgcccgag acaccggagc atggaaggcg tgctccgtgg tcaggtcctc
     2401 ccatagggtc gcgcgaacag ggggggcgtg tcgccaccgg cagcatccat ccgggacccc
     2461 cgctcacctc catcccggcg tacccgctgg cttccagcta ccgggactcc aggtacctca
     2521 ggacggccag gacgcgtcgg tgatcatcgg gtgcctccgc gagcgccagc ttggccagga
     2581 tgctgcgcac gtgcttctcg acggtgccct cggcgagcca cagacgagcc gcgatcccgg
     2641 cgttcgatcg tccctccgcc atcagggcga ggacctcccg ctcccgatcg gtgagggtct
     2701 cgagggggtc gtggctgcgt cgtgccccca ccaactcctg gaccacctcg ggatcgatca
     2761 ccgtgccgcc atcgaggatc tgctccaccg atgcgaggaa gctctggacg tcggtgaccc
     2821 ggctcttcag cgtgtacccg agccggtctc cactggccaa gagctccatg gcgtgctcga
     2881 cctcgacgtg tgccgacagc accatgatgg cgacctccgg gaagtcctga cggatcagcc
     2941 gagccgcgtc cagtccctcc gtcgagtgac cggggggcat ccggatatcc acgatggcca
     3001 gatcgggatg ctccgcccgg accagcgaga ggagctccga ggcggtcccg gcccggccca
     3061 ccacccgtga cccggcccgc tcgaagacgg tggccagccc ttcccgcagg aggacgtcat
     3121 cgtccgccag cacgacgcgg gccggcctca tcgaacgatg aaggtggttc caggaccggg
     3181 tcgcacggtg gggccgagca tggtacgcat ggaccggtcc ctccccggat gctgccgtgt
     3241 gaagcttgtc gtcgcgggga cgggatttga acccgtgacc tctgggttat gagcccagcg
     3301 agctaccgga ctgctccacc ccgcaccccg atgctaccga ccgagtgggg ccatcgccaa
     3361 ccatcggccc ggatcctcct gccggcccgc acgcatgaaa ccgccggcag gcgggaaccc
     3421 ccgcggcatg agggtcaagc cgatccttgc ggtggcggcc gtggccctgg tggcggcggc
     3481 ctgctcgagt tccggtccaa cgaccgcacc caccgcatcg ccgagcgcgg cctcaccctc
     3541 cgtgtcgcct gcggccacac cgagcgaggg cgcctcgccc agcgcccaga gccccgcagc
     3601 cgccaaggcc gagatcgagc gtaactggac ccggttcttc aacgggcaga ccccgcccgg
     3661 ccagaagatc gatctgctgg tcgacggaca gcagttcgcc ggggtgatcg aggcttcgtc
     3721 gcagtctcag ctcgcccggt ccaccagggc caaggtgacg aacgtctccc tgaccggtcc
     3781 cgacacggcc accgtcacct acgacgtcct ggtgggcggt cagccggcgc tgaccgatca
     3841 gcagggcacg gcggtccgca cggacggcac ctggagggtc agcgacgcga gcttctgctc
     3901 cctgctcgcg ctcggcggtc aggtccccgc ggcctgtccc tcgccggggg cgagcccgtc
     3961 ggcctcggga tgagggcacc ggcgggacgg ggccgaggtc gccggctggc cacgctcgcc
     4021 acgatcctgt tcctcacctt cctggacaac acgatcgtca gcgtgatcct cgccgacatg
     4081 cagaccaccc tgcatccggg cgtgtccggc ctgcagtgga tcgtgaacgg ttacatgctc
     4141 gccttcgccg ccctgatgct gaccggtggc acgctcggtg acctgctggg ccgcaagaag
     4201 gtcatgttgt ccggggtggc ggtgttctgc gccggatccg tggccgccgc gctggccccg
     4261 accacctcgg tgctgatcgg cagccgcgtg gtgatgggga tcggcgccgc cgcctcagag
     4321 ccgggcaccc tgtcgatgat ccgccacatc taccctgagc gcggtccccg agcccgggcg
     4381 ctgggcgtct gggcggccgt gtcgggggtg gctctggccc tggggccgat cgtgggtggc
     4441 gtcctggtgg ggtacagcgg gtggcggtcc gtgttctggt tcaacctggg cttcggcgct
     4501 gcggcgctgg cggccggggt cctgctgctg cccgagaacg ccgatccgga aggacgacgg
     4561 ctcgacgtgc cgggcctgat cgtgggagcc gtcaccatct ccgcgatcgc gatcgccgtg
     4621 atcgagggcg agaacgccgg ctacctctcc tggtggatcg acctgctgtt cgccgtggct
     4681 gcgctgggcg tggtggcgtt catccggatc gagcgccgga gccccgatcc cgtcctgaag
     4741 ctgcacttct tccagacctc agcgttctcg ggggccaacg tcgtggcgtt cgccaccaac
     4801 ttcggcgtct tcgccgtgtt cttcttcacc gcgttgtacc tgcagttggt ggccgacttc
     4861 tccggctacc ggatcgcgct gcagttcgtc accatggcgg cggccatggt cgccgccgct
     4921 ccgctggccg ggcggtgggt ggcggctcga gg
//
LOCUS       DASQQQ010000074         9050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_87885, whole genome shotgun sequence.
ACCESSION   DASQQQ010000074 DASQQQ010000000
VERSION     DASQQQ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9050
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87885"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VE646_03200"
     CDS             <1..470
                     /locus_tag="VE646_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZD79035.1"
                     /translation="AGMAAALTACLDDMADEAARRWGLTRRLREGLGSIPRVQLHGHP
                     TQRVPHLACFSVDAVDAEILAMALDERGFRLSVGSNCSGAPGEPSPILEQMGIPNTAS
                     FRLGVGRETGSEDVEALIEVLPGLIQELRAVEEAAEAAMARFRPSPEDPSAPV"
     gene            complement(498..1544)
                     /locus_tag="VE646_03205"
     CDS             complement(498..1544)
                     /locus_tag="VE646_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018528287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HZD79036.1"
                     /translation="MSDVQGAAGNGQEAGVGTLTKKDFTSDQEVRWCPGCGDYAILAT
                     VQSFMPELGIEPDRTVFVSGIGCSGRFTYYMDTFGIHGIHGRAPAIATGIATARPDLN
                     VWVITGDGDALSIGGNHLIHALRRNVNLKILLFNNQIYGLTKGQYSPTSEEGKVTKST
                     PFGSLDHPFNPIALALGAEATFVARTIDNDRQHFTQVLRQAAAHEGAAFVEIYQNCNV
                     FNDGAFDILREKPQGAHNQIRLEHGQPILFDEGNRCVAVGHNGRLVIANVDEVDSGQI
                     LVHDAHGRPSLAFALAHVSHGPSEPTCIGVFREVQRPVYGEAMERQIEMATERLGAGS
                     LEQLLHGGDTWVVG"
     gene            complement(1541..3379)
                     /locus_tag="VE646_03210"
     CDS             complement(1541..3379)
                     /locus_tag="VE646_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HZD79037.1"
                     /translation="MSKAVEEKERVVIRFAGDSGDGMQLTGDRFTNATAILGNDLATL
                     PDFPAEIRAPAGTVHGVSAFQIQFASEDILTPGDHPNVLVAMNPAALKANLETLEKGG
                     TVIVNEDAFIRRNIDKAGYAENPLEDGTLDGYQVYRVPMTSITVRAVEELGVSRKEAE
                     RSKNMFALGLVSWMYGRPTDVTLSWLERKFGEKPLVLDANVAAFKAGYNFGETTELFA
                     HSYRVKPAPATPGTYRNVSGATALALGLTAASQRSGLPLFYASYPITPASELLHELSR
                     LKNFGVRTLQAEDEIAAAGIALGASFAGDLGVTGTSGPGMDLKEETLGLAVALELPMV
                     IVDVQRAGPSTGMPTKTEAADLLMAMFGRHGESPMPIVAAYSPGHCFDAALEASRIAV
                     TYRTPVILLSDTFLGNSSEPWRLPDVGSLPTIEPDFAQGPNAGTEFLPYLRDENLARP
                     WAVPGTPGLRHRLGGLEKDEASHISYDPHNHERMTHLRAAKVDKVADGIPPLEVDDPD
                     GDAELLVLGWGSSYGTIRAAVRRVREAGHKVAAAHLVHLNPFPRNTGDVVRSYRHVLI
                     PEMNMGQLALLIRARFLVDAKTYSKVQGVPIFAEELENEILKVMNG"
     gene            3518..4459
                     /locus_tag="VE646_03215"
     CDS             3518..4459
                     /locus_tag="VE646_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZD79038.1"
                     /translation="MKTHRLGSNGPELSVVGFGAWEAGGGSEWGQSPPEEQVVQAIRT
                     VPDTGIDWIDTAEVYGDGRSEELVGRAIAGRRDELFIATKVAPSPEGSGFRPEEIAKA
                     VRGSLQRLGIDHIDLYQLHWPDETGIPVEDSWGAMATLVDDGLVRFIGVSNFDRELIE
                     RCEPIRHVDSLQQEFSMLALDDRELIRWCGEQGTGVVTYGPLAYGLLTGKITMETAFD
                     RDDHRSQDDRIFPPDKRERSLAVVDGMRPIAERLGISLAQLALAWNVHQPGVTSAIAG
                     SRDPGHVRANAVAGDVELDADARSELERLVTQGPATA"
     gene            4489..5514
                     /locus_tag="VE646_03220"
     CDS             4489..5514
                     /locus_tag="VE646_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZD79039.1"
                     /translation="MAYRRIVLATDGSDSAETPEHVAMTLAKATKGRVTVVNAYRNPE
                     RAEPAVRRAREIGEREGVKTEVVLSPEEPDRAIVATAEDKQADVIVIGSRGLYKGEQL
                     VGNVVRKVATHAPCDVLLTRERAAERRPHGEPPYRRLLIASDGSATADRAARKGYALA
                     KRLGASVTLLFVGHPTTGEMVLQDTIATIGDQEVPSSILIRDGDAAQVIIDTASSEGF
                     DLVVVGNRGLAGAKAMILGSVPRSVTEYATCDVLVARTIAQSLDEIGRGEGGIVTTGD
                     HKVAVYRDERGHLTTLSAKCTHMGCTVKWNPTERTWDCPCHGSRFSGVGEVVNGPAAR
                     PLDPTTL"
     gene            complement(5546..6031)
                     /locus_tag="VE646_03225"
     CDS             complement(5546..6031)
                     /locus_tag="VE646_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HZD79040.1"
                     /translation="MLALSIDYRWLVFIHIVGVLGFVMFHGVSVVVALRLRKERDRAR
                     IAELMQFSGSSIAGMYVSLLILLVGGVWAGFRLDWWRYAWIWVALGVLVFMIAEMSAV
                     ARPYYQRVKEAVEVRPSGVPRRSDEELEQMLGSALPLINAAAGFAALLFIAWLMVYKP
                     W"
     gene            complement(6031..6555)
                     /locus_tag="VE646_03230"
     CDS             complement(6031..6555)
                     /locus_tag="VE646_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZD79041.1"
                     /translation="MKDVFDPLSGTVPGSIAGTDVRRRRSSAGPRPDRPLGKEGVRLL
                     GAVPLFSGLSKRHLRKLAERADEVAFAKGERIVGTGLLGGAFFVIIEGEAKVTKGKRV
                     LTALGPGDFFGELALLDAGERLASVTAATPMRAVRLFKRSFDRLLAEEPGVAVKMLSV
                     LAARIRRVEQSLTG"
     gene            6678..>9050
                     /gene="metH"
                     /locus_tag="VE646_03235"
     CDS             6678..>9050
                     /gene="metH"
                     /locus_tag="VE646_03235"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HZD79042.1"
                     /translation="MSGTARAEAARGPLERIFAERIAILDGAMGTAIQARGLGEGDFR
                     GRRLRDHPVDLRGDNDLLCLTRPDVVLEIHRGHLRAGADIISTNTFNANPFSQADYGL
                     QDLVFEINRAGAELATRAAEEWAPDRFVAGSVGPTNQTLSLSPRVNEPAYRAVTFDEM
                     FAGYAEQIRGLVAGGVDLLLIETIFDTLTAKAAIVAAMEVAPNVPLMISVTITDRSGR
                     TLSGQTIDAFWASVQHARPFTVGVNCSLGAAEMRPYLEALARAAPVFTTCYPNAGLPN
                     AFGGYDEQPDTTGKYLREFAEAGLANVLGGCCGTTDRHIRRIADVVEGLPPRTPPAAR
                     ELTVFSGLEPFAILPDTGFVMIGERTNLTGSKRFRRLIGSGDYQGAVDVAMEQVRGGA
                     NMLDVNMDADLLESEQAMTTFLNLIATEPEIAKVPVMVDSSKWSVLEAGLKCLQGKGV
                     VNSLSLKEGEDDFLQKARKVARYGGGVVVMAFDEQGQADTVGRKIEICSRAHRLLVKE
                     AGYRDEDIIFDPCILAIATGIEEHDEYAKAFIEATRELKRRFPRCRVSGGVSNLSFSF
                     RGNDVVREAMHAAFLYHAIQAGLDMAIVNAGQLAVYEDIPPDLLEHVEDVIFHRRPDA
                     TERMVAFADTVKGEGAKRELDLGWRDAPVQQRLSYALVHGIVDFIEADTEEARRSYPR
                     PLDVIEGPLMDGMRIVGDLFGAGKMFLPQVVKSARAMKKAVAYLEPFMEDEKVRIGGD
                     HAQGKVVLATVKGDVHDIGKNIVGVVLQCNGYEVIDLGVMVPSERILETAR"
BASE COUNT         1433 a         3122 c         3147 g         1348 t
ORIGIN      
        1 tggcgggcat ggcggcggcc ctgaccgctt gcctcgacga catggccgac gaggccgcgc
       61 gccggtgggg cctgacccga cggctgcgcg aggggctcgg ttcgatcccc cgggtccagc
      121 tccacggcca ccccacccag cgggttcccc acctggcctg cttctccgtg gatgcggtgg
      181 acgccgagat cctcgccatg gccctggacg agcgcgggtt ccggctttcg gtggggtcca
      241 actgctccgg cgccccgggg gagccatccc cgatcctcga gcagatgggc atccccaaca
      301 ccgcgagctt ccggctcggc gtgggccgcg agaccggctc ggaggacgtg gaggccctca
      361 tcgaggtcct tccggggttg atccaggagc tccgcgcggt ggaggaggcg gccgaagccg
      421 cgatggccag gttccgcccc tcgcccgagg acccatcggc cccggtctga gcccgccccg
      481 ctttcgggcg ggcccgctca gccgaccacc caggtatccc cgccgtgcag gagctgctcc
      541 aggctgcccg cccccaggcg ctcggtggcc atctcgatct ggcgctccat ggcctcgccg
      601 tagaccggcc gctgcacctc gcggaagaca ccgatgcagg tgggctcgct cgggccgtgg
      661 gagacgtggg ccagggcgaa ggccagcgag gggcgcccgt gcgcgtcgtg gaccaggatc
      721 tgcccgctgt ccacctcgtc cacgttcgcg atcaccagcc gaccgttgtg ccccaccgcc
      781 acgcaacggt tcccctcgtc gaacaggatc ggttgcccat gctcgagccg gatctggttg
      841 tgcgcgccct gcggcttctc ccgaaggatg tcgaaggccc cgtcgttgaa cacgttgcag
      901 ttctggtaga tctcgacgaa cgcggcgccc tcgtgggcgg ccgcctgccg gagcacctgc
      961 gtgaagtgct gccggtcgtt gtcgatggtc ctggccacga acgtggcctc ggccccgagc
     1021 gccagggcga tcgggttgaa gggatggtcc agggagccaa agggtgtgga tttggtgacc
     1081 ttgccctcct ccgacgtcgg cgagtactga cccttggtga ggccgtagat ctggttgttg
     1141 aacagcagta tcttcaggtt cacgttccgc cgcagagcgt ggatcaggtg gttgcctccg
     1201 atcgagagcg cgtcaccgtc tccggtgatc acccagacgt tcaggtcggg ccgagccgtg
     1261 gcgatccccg tggcgatggc cggggctcgc ccgtggatcc cgtggatccc gaaggtgtcc
     1321 atgtagtacg tgaaccggcc cgagcacccg atcccggaga cgaacacggt gcgatcgggc
     1381 tcgatcccca gctcgggcat gaacgattgc accgtcgcca ggatcgcgta gtcgccacat
     1441 cccgggcacc agcgcacctc ctggtcgctg gtgaagtcct tcttcgtcag cgtgccgacg
     1501 ccggcctcct gaccgttgcc ggcggctcct tgcacatcac tcatccgttc atcaccttca
     1561 ggatctcgtt ctccagttcc tccgcgaaga tcggcacccc ctggaccttc gagtaggtct
     1621 tcgcgtccac caggaagcgc gccctgatca gcagggcgag ctggcccatg ttcatctcgg
     1681 ggatcaatac gtgccggtag gaacggacca cgtcgcccgt gttcctgggg aaggggttga
     1741 ggtgcaccag gtgggccgcc gccaccttgt ggcccgcctc gcggacccgc cggaccgccg
     1801 cccggatcgt gccgtaggac gaaccccacc ccagcacgag cagctcggca tcgccgtctg
     1861 ggtcgtcgac ctcgagcggt gggatgccgt cggccacctt gtccaccttc gcggcgcgca
     1921 ggtgcgtcat gcgctcgtgg ttgtgcgggt cgtagctgat gtggctggcc tcgtccttct
     1981 ccaggccgcc caggcggtgc cggaggcccg gtgtgcccgg gacggcccag ggacgcgcca
     2041 ggttctcgtc gcgcaggtac gggaggaact cggtgcccgc gttcggaccc tgcgcgaagt
     2101 ccggttcgat cgtgggcagc gagccgacgt cgggcagccg ccacggttcg gacgagttcc
     2161 ccaggaaggt gtccgagagc aggatcaccg gggtccggta cgtgacggcg atccggctcg
     2221 cctccagggc agcgtcgaag cagtgccccg gggagtacgc cgccacgatc ggcatgggcg
     2281 actccccatg ccggccgaac atggccatca gcaggtcggc cgcctcggtc ttggtgggca
     2341 tcccggtgga gggaccggcc cgctggacgt ccacgattac catcggcagc tccagggcca
     2401 cggccagccc cagggtctcc tccttcaggt ccatgcccgg tccgctggtc ccggtgacgc
     2461 cgaggtcccc ggcgaacgag gcgccgaggg cgatccctgc agcggcgatt tcgtcctcgg
     2521 cctggagcgt ccgcacgccg aagttcttca gtcgcgacag ctcgtgcagg agctcggagg
     2581 ccggcgtgat gggatagctc gcgtagaaga ggggcagtcc gctccgctga ctggccgcgg
     2641 tgagcccgag ggccagcgcg gtggctccgg agacgttgcg gtacgtgccg ggcgtggcgg
     2701 gggccggctt cacccggtag ctgtgcgcga acagctcggt cgtctcgccg aagttgtagc
     2761 cggccttgaa ggccgccacg ttggcgtcca gcaccagcgg cttctcgccg aacttccgct
     2821 ccagccagct gagggtcacg tccgtaggtc ggccgtacat ccacgagacc agccccagcg
     2881 cgaacatgtt cttcgagcgc tcggcctcct tccgggagac cccgagctcc tccacggccc
     2941 gcaccgtgat ggaggtcata ggcacccggt agacctggta cccgtccagg gtgccgtcct
     3001 cgagggggtt ctcggcatag cccgccttgt cgatgttgcg gcggatgaag gcgtcctcgt
     3061 tcacgatcac cgtgccgccc ttctcgaggg tctcgaggtt ggccttgagg gcggcggggt
     3121 tcatggccac cagcacgttc gggtgatccc ccggcgtgag gatgtcctcg gacgcgaact
     3181 ggatctggaa cgccgaaacg ccgtgcaccg tccccgccgg cgcccggatc tcggccggga
     3241 agtcggggag ggtggccagg tcgttgccca ggatggcggt ggcattggtg aaccgatcgc
     3301 cggtcaactg catcccgtcg cccgagtcgc cggcgaaccg gatgacgacg cgttctttct
     3361 cttcaacggc cttgctcatg gctccgtggc cgggcggcgc ccggccgcct ccttctgttg
     3421 actcgcgggt tcgtcaccta gcgtaccgcc tgaccccgga agggacagtc aggagcggta
     3481 cattgtgccg gtccgtatgc cgggagggta tcttgacatg aagacacatc gcttgggaag
     3541 caacggcccg gagctgtccg tggtcggttt cggggcctgg gaggcgggtg gcggcagcga
     3601 atggggccaa tcccccccgg aggagcaggt ggtgcaggcg atccgcaccg tgcccgacac
     3661 ggggatcgac tggatcgaca ccgccgaggt ctacggtgac ggacgctcgg aggagctggt
     3721 gggccgggcc atcgccgggc ggcgagacga gctgttcatc gccaccaagg tcgcgccctc
     3781 cccggaggga tccgggttcc ggcccgaaga gatcgccaaa gccgtccggg ggagcctgca
     3841 gcgactgggg atcgatcaca tcgacctcta ccagcttcac tggccggacg agaccggcat
     3901 ccccgtggag gactcgtggg gcgcgatggc cacgctggtc gacgacgggc tggtgcgctt
     3961 catcggagta tcgaacttcg accgggagct gatcgagcgc tgcgagccga tccgccacgt
     4021 cgattccctg cagcaggagt tctccatgct ggccctggac gatcgcgagc tgatccgctg
     4081 gtgcggggag cagggcacgg gcgtggtcac ctacggaccg ctcgcctatg gcctgctcac
     4141 cggcaagatc accatggaga ccgccttcga tcgggacgac caccgcagcc aggacgaccg
     4201 gatcttcccc ccagacaagc gggagcggag cctggcggtc gtcgacggga tgcgtccgat
     4261 cgccgagcgg ctgggcatca gcctggcgca gctggcgctg gcctggaacg tccaccagcc
     4321 gggcgtcacc tcggccatcg ccgggagccg agacccggga cacgtccgcg cgaacgcggt
     4381 agccggcgac gtcgagctgg acgcggatgc gcgatcggag ctggagcgcc tcgtgaccca
     4441 gggcccggcc acggcgtaag gcgcgccacc gaagggagcg acagcgagat ggcctaccgg
     4501 cggatcgtgc tcgccacgga cggctcggac tcggccgaga caccggagca cgtagcgatg
     4561 accctggcca aggcgacgaa gggccgggtc accgtagtga acgcctatcg gaaccccgag
     4621 cgagcggagc ccgcggtccg ccgagcccga gagatcgggg aacgggaagg cgtgaagacc
     4681 gaggtggtcc tctcccccga ggagcccgac cgagcgatcg tggcgacggc cgaggacaag
     4741 caggccgatg tcatcgtgat cgggagccgc ggcctgtaca agggcgagca gctcgtgggc
     4801 aacgtggtcc gcaaggtggc cacgcacgcg ccctgcgacg tcctgctcac ccgcgagcgc
     4861 gccgcggaac gtcgtccgca cggcgagccc ccctaccggc gcctgctgat cgcctccgac
     4921 ggctcggcca cggccgaccg cgctgcccgc aagggctacg cgctggccaa acgactcggt
     4981 gcttcggtca ccctgctctt cgtgggtcac cccaccaccg gcgagatggt cctgcaggac
     5041 accatcgcca cgatcggcga ccaggaggtc ccgtcctcca tcctgatccg cgacggagac
     5101 gccgcccagg tcatcatcga caccgcgtcg agcgaggggt tcgacctggt ggtggtgggc
     5161 aaccgcggct tggccggggc caaggccatg atcctgggct cggtcccccg cagcgtgacc
     5221 gagtacgcca cctgcgacgt cctggtggcg cgcacgatcg ctcagagtct ggacgagatc
     5281 ggcaggggcg agggcggcat cgtgaccacc ggcgaccaca aggtggccgt ctaccgggac
     5341 gagcggggcc acctcaccac cctgtccgcc aagtgcaccc acatggggtg caccgtgaag
     5401 tggaacccca cggaacgcac gtgggactgc ccctgccacg gctcacgctt ctcgggggtg
     5461 ggcgaggtgg tgaatggtcc ggcggcccga ccgctcgacc ccaccacgct gtagcatgcg
     5521 ccggcgccac cccggccgga tggggtcacc agggcttgta gaccatgagc caggcgatga
     5581 acagcagcgc cgcgaagccg gccgccgcgt tgatcaaggg cagcgccgag ccgagcatct
     5641 gctcgagctc ctcgtcgctg cggcggggca ccccggacgg ccgcacctcg acggcctcct
     5701 tgacccgctg gtagtaggga cgggcgacgg cactcatctc ggcgatcatg aacaccaaca
     5761 cgccgagcgc cacccagatc cacgcgtagc gccaccagtc gagacggaac ccggcccaga
     5821 ccccgccgac cagcaggatc aggagcgaga cgtacatccc ggcgatcgag gacccggaga
     5881 actgcatcag ctcggcgatc ctggcccggt cgcgttcctt gcgcagccgc agggccacca
     5941 ccaccgatac cccgtggaac atcacgaaac cgagcacgcc cacgatgtgg atgaagacca
     6001 gccagcggta gtcgatcgag agggcgagca tcaaccggtc agcgactgct ccacgcggcg
     6061 gatccgcgcg gcgaggacgg agagcatctt gactgccacc ccaggttcct cggccaggag
     6121 ccggtcgaag gaccgcttga acagccggac cgcgcgcatc ggggtcgcgg cggtcacgct
     6181 ggccaagcgc tcgccggcgt ccaggagggc gagctcgccg aagaaatccc cggggccgag
     6241 ggcggtcagc acccgcttgc ccttcgtgac cttcgcctcc ccctcgatga tcacgaagaa
     6301 ggcgccgccc aacaggcccg tgccgacgat ccgctccccc ttggcgaatg ccacctcgtc
     6361 cgcccgctcg gccagcttgc ggaggtggcg cttcgacagg ccggagaaca agggcaccgc
     6421 ccccagcagc cgcacccctt ccttgcccag cggccggtcc ggccgcggcc cggcggagga
     6481 gcgacggcgg cgaacgtcgg taccggcgat cgagccgggc accgtccccg agagtggatc
     6541 gaacacgtcc ttcacccgaa cctcctgacc gccagggacg agacggcagc ctaccgcacg
     6601 ggacctgtgg gggggagggt ccgtggaagt ggtggtcgtg cggctccggc cactggggat
     6661 gggtacgatg actggtcatg agtgggacgg cgcgggccga ggcggcgcgc gggcctctgg
     6721 aacgcatctt cgccgagcgc atcgcgatcc tcgatggggc gatgggcacc gcgatccagg
     6781 ctcgaggtct gggcgagggc gacttccggg gccggcgact gcgggatcac cccgtggatc
     6841 tgcgcggtga caacgacctg ctctgcctga cccgccccga cgtcgtcctc gagatccatc
     6901 gcggccacct gcgggcggga gccgacatca tcagcaccaa caccttcaac gcgaacccct
     6961 tctcccaggc cgactacggg ctgcaggacc tcgtcttcga gatcaaccgc gcgggcgccg
     7021 agctcgccac gcgcgcggcc gaggagtggg cccccgatcg gttcgtggcc ggatccgtgg
     7081 ggccgaccaa ccagaccctg tcgctcagtc cccgggtcaa cgagcccgcc taccgcgcgg
     7141 tcaccttcga cgagatgttc gcagggtacg ccgagcagat ccgggggctg gtggcggggg
     7201 gggtcgatct cctgttgatc gagaccatct tcgacacgct gaccgccaag gccgccatcg
     7261 tcgccgccat ggaggtggcc cccaacgtcc cgctgatgat ctcggtgacc atcacggacc
     7321 ggagcgggcg cacgctctct ggccagacga tcgacgcgtt ctgggcatcc gtgcagcacg
     7381 cccgtccctt caccgtaggg gtgaactgct ccctgggggc ggcggagatg cgtccctacc
     7441 ttgaggcgct ggcccgcgcg gcgccggtct tcaccacctg ctacccgaac gcgggactgc
     7501 ccaacgcgtt cggaggctac gacgagcaac ccgacaccac cggcaagtac ctgcgcgagt
     7561 tcgcggaggc cgggctcgcc aacgtcctgg ggggatgctg cggcaccacc gaccgccaca
     7621 tccgccggat cgccgacgtg gtggagggtc tgccgccccg gaccccgccc gccgcccgcg
     7681 agctcacggt cttcagcggg ctcgagccgt tcgcgatcct ccctgacacg ggtttcgtga
     7741 tgatcggcga gcgcaccaac ctcacgggct ccaagcggtt ccgccgcctc atcggatcgg
     7801 gcgactacca gggcgccgtg gacgtagcca tggagcaggt gcggggcggc gcgaacatgc
     7861 tcgacgtgaa catggatgcg gacctgctcg agtccgagca ggccatgacc accttcctga
     7921 acctgatcgc cacggagccg gagatcgcca aggttccggt catggtggac agctcgaagt
     7981 ggtcggtctt ggaggccggc ttgaagtgtc tgcagggcaa gggcgtcgtc aactccctca
     8041 gcctgaagga aggagaggac gacttcctcc agaaggcccg gaaggtggcc cggtacgggg
     8101 gcggcgtggt ggtcatggcg ttcgacgagc aggggcaggc cgacaccgtc gggcgcaaga
     8161 tcgagatctg ttcccgtgcc caccgcctct tggtgaagga ggccgggtac cgggacgagg
     8221 acatcatctt cgacccgtgc atcctggcca tcgccaccgg catcgaggaa cacgacgaat
     8281 acgcgaaggc attcatcgag gccacgcgtg agctcaagcg acggttccct cggtgccggg
     8341 tctccggcgg cgtctcgaac ctgtcgttct ccttccgcgg caacgacgtc gtccgggagg
     8401 ccatgcacgc cgccttcctc taccacgcga tccaggccgg gctcgacatg gccatcgtca
     8461 acgccgggca gctggccgtg tacgaagaca tcccccccga cctgctggag cacgtggagg
     8521 acgtgatctt ccaccggcgt cccgacgcta cggagcggat ggtcgccttc gcggacacgg
     8581 tgaaggggga gggcgccaag cgtgagctgg acctgggctg gagggacgcg ccggtgcagc
     8641 agcggctcag ctacgcgttg gtgcacggga tcgtcgactt catcgaggcg gacaccgagg
     8701 aggcgcgccg ctcgtacccg cgcccgctcg acgtgatcga gggaccactg atggatggca
     8761 tgaggatcgt gggcgacctg ttcggtgcag gcaagatgtt cctgccccag gtggtgaaga
     8821 gcgcccgggc catgaagaag gcggtcgcct acctggagcc cttcatggag gacgagaagg
     8881 tgcgcatcgg tggcgatcac gcacagggca aggtggtgct cgccaccgtg aagggcgacg
     8941 tccacgacat cggcaagaac atcgtcggcg tggtgctcca gtgtaacggt tacgaggtca
     9001 tcgacctggg cgtgatggtg ccgtccgagc gcatcctcga gacagcgcgc
//
LOCUS       DASQQQ010000075         3804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_143722, whole genome shotgun sequence.
ACCESSION   DASQQQ010000075 DASQQQ010000000
VERSION     DASQQQ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143722"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VE646_03240"
     CDS             complement(<1..362)
                     /locus_tag="VE646_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79043.1"
                     /translation="MAESGRNASKLLKTLEAAGDVEVDRSVVGAVSGRDVRLHQAAAG
                     AVMASGDVTFERGGSGPVLAGGSVRFTQGGCGPVLTGGGVSFEQGGCQSVIAGGGVTV
                     GNRGFVGLALSPRLEVHDG"
     gene            782..854
                     /locus_tag="VE646_03245"
     tRNA            782..854
                     /locus_tag="VE646_03245"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:815..817,aa:Val,seq:tac)
     gene            991..1260
                     /locus_tag="VE646_03250"
     CDS             991..1260
                     /locus_tag="VE646_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79044.1"
                     /translation="MREERLWHPLSRFHGWLSRFHLWIWMNEELIVIGALSVLVIGAI
                     GLIVVSGHTPVPPQSPFDRAPVVQPENMPVPGPTSHPQPAELPAH"
     gene            1342..2829
                     /locus_tag="VE646_03255"
     CDS             1342..2829
                     /locus_tag="VE646_03255"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZD79045.1"
                     /translation="MADAPFELQEVTVAELARRMQEGEVTARALTDAYLDRIDALDRD
                     GPQLRSVIATNPTAQAAAEELDRERQQGHVRGPLHGIPILVKDNIDISGPMGTSAGSL
                     ALREARPSSDAPLVRRLLDAGVVILGKANLSEWANFRSTRSSSGWSAMGGQCRNPYVL
                     DRNPCGSSSGSAVAVSADLCVVAVGTETDGSIMCPASIDGIVGIKPTVGLVRGDGIVP
                     ISHTQDTAGPMARTVADAFELLSAMAEAAPGRGLASDRPSPLDPKALRSARIGVARNL
                     AGFHDRVDALFEQALSALRDLGAELVDAADVPHATELEEPELDVLLYEFKADLETYLT
                     GVDGDVPRTLEGLIAFNRAHADEELAFFGQELFEKAAAKGPLTEPAYLEALSTCGRLA
                     RDEGLDAAFTAHGVDAIVAPTGAPAWLTDHVNGDHYVGGNTTPAAVAGYPSVTVPMGF
                     VSELPVGLSFIGHPWTEATLAGYAYAFEQATTERRPPRYLPTSEA"
     gene            complement(2860..3072)
                     /locus_tag="VE646_03260"
     CDS             complement(2860..3072)
                     /locus_tag="VE646_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HZD79046.1"
                     /translation="MAESIYRVTQVIGTSTESWEEAAKHAVETAAKSLRDLRVGEVDR
                     LDLTIEDGRVTHYRARVNISFKYEEH"
     gene            complement(3135..>3804)
                     /locus_tag="VE646_03265"
     CDS             complement(3135..>3804)
                     /locus_tag="VE646_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017198776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZD79047.1"
                     /translation="DAVEIAFTGLDHGIEGGVEQDYGRSLSVEQAMREEVLLAWEMNG
                     EPLPPQHGFPLRLLVPGWYGMTSVKWLTRIEAVTEPFAGYQQAEAYRFQQSEDDPGQP
                     VTRIRVRALMVPPGIPDFFTRARTVDAGPVVLVGRAWCGAAAVERVEVGVDGGWFEAT
                     LQEPVGDWAWRGWSFTWDAMPGEHTLACRATDANGDTQPLEQPWNVQGMANNLVQQVP
                     VTVR"
BASE COUNT          519 a         1392 c         1306 g          587 t
ORIGIN      
        1 ccgtcgtgca cctcgagccg aggggacagg gccaggccca cgaagccccg gttgcccacg
       61 gtgacgccgc cgccggcgat cacgctctga caccccccct gttcgaacga gacgccgccg
      121 cccgtgagca ccggcccgca gcctccctgc gtgaaccgaa cgctgcctcc ggccaacact
      181 ggcccgctgc ccccgcgctc gaacgtgacg tctccgctgg ccatcacggc gcccgccgcc
      241 gcctggtgca agcgcacgtc ccgaccgctc accgcgccca ccaccgagcg gtccacctcc
      301 acgtcgccgg ccgcttccag ggtcttcaag agcttgctcg cgttccgtcc gctctcggcc
      361 atctgcagac cacctcctcg ctccctccca tcctagggaa ccggtgccgg atgccgtggt
      421 gggggctatc ctgacccggt cgtgaccgac gacgccatcc ccctcggttc cgcgtccccg
      481 ggacgcccgg gatcggagcg cccgaggtgc ttcctgtgcg ggcgttcgac cttcgacccc
      541 cccaaggggg ggcgtccgtg ggtgcgcgcc gcggtggccg gtcagcaggc gctcgtgtgc
      601 cctcgttgcc aggaagagcg cctcgactgg ctcgtccacc tcgatcggtg cgaacgctgc
      661 ggtgccaccc gtctgtcggt catgctcgga gaagtggtct gtcgtgcctg cggccatgtc
      721 cgcggctccc aggtgggccc ggccctctga ccgcgtcggg gccggtagca tctgccacac
      781 ggggcgctta gctcagcggt gagagcgctg cccttacaag gcagaggacg gtggttcgaa
      841 tccaccagcg cccacgtcag gcggccgggt gcgggtctgc ttccaccggc ctcgccgcgt
      901 gggacgcgga tgcgataggg tgtggtggct ctggtcgccc ccgccgtcac cccatcctgt
      961 gcgagcgcga tgggggggta gaggaagaac gtgcgcgaag aacggttgtg gcatcccctg
     1021 tcccgcttcc atgggtggct ctcccggttc cacctgtgga tctggatgaa cgaggagctc
     1081 atcgtgatcg gggccctgtc cgtgctggtc atcggggcga tcgggctcat cgtcgtctcc
     1141 gggcacacgc ccgttcctcc ccagtcgccg ttcgaccggg cgccggtggt gcagcccgag
     1201 aacatgccag tccccgggcc gacgagccat ccgcagccgg ccgagctccc cgcccactga
     1261 gcgcccgccc ggtcgacggg ttggctgtga gccgggtcac ccgcacggcc gggccgacga
     1321 tgcggtagcg tcccggtggt catggccgat gcgccgttcg agctgcagga ggtcacggtc
     1381 gccgagctgg cccgccggat gcaggagggc gaggtcacgg cgcgggccct caccgacgcc
     1441 tacctggacc ggatcgatgc gctggaccgg gacggcccgc agctccggtc cgtgatcgcc
     1501 acgaatccca ccgcccaggc cgccgccgag gagctggatc gcgagcggca gcaaggccat
     1561 gtacgtgggc cgctgcacgg gatccccatc ctggtcaagg acaacatcga catctcgggg
     1621 cccatgggga ccagcgccgg ctcgctggct ctccgcgagg cccgtccttc gagcgatgca
     1681 ccgctcgtcc gccgactgct ggacgccggc gtggtgatcc tgggcaaggc gaacctgtcc
     1741 gagtgggcca acttccgctc cacgcgctcc tcgagcggct ggagcgccat gggcggtcag
     1801 tgccggaatc cttacgtgct cgatcgcaac ccgtgcgggt cgagctcggg ctccgccgtc
     1861 gcggtatcgg ccgatctatg cgtcgtcgcc gtcggtacgg agaccgacgg gtcgatcatg
     1921 tgtccggcgt cgatcgacgg gatcgtcggg atcaagccga cggtggggct ggtgcggggg
     1981 gacggcatcg tgcccatctc gcacacgcag gacacggcgg ggccgatggc acgcacggtg
     2041 gccgacgcgt tcgagctcct gtcggcgatg gcggaggccg caccgggccg gggcctggcc
     2101 tcggatcggc catcaccgct cgacccgaag gccctgcgga gcgcgaggat cggtgtggct
     2161 cggaacctgg cggggttcca cgaccgggtg gatgcgctgt tcgaacaggc gctgagcgcg
     2221 ttgcgggatc tgggggccga gctggtggac gcggccgacg ttccccacgc caccgagctg
     2281 gaggagcctg agctcgatgt cctgctctac gagttcaagg ccgatctgga gacgtacctg
     2341 accggcgtgg acggcgacgt tccccgcacc ctcgaggggc tcatcgcctt caaccgggct
     2401 cacgcggacg aggagctggc gttcttcggt caagagctgt tcgagaaggc ggcggcgaag
     2461 ggtccgctga ccgagcccgc gtacctcgag gccctctcga cctgcggccg cctggctcgg
     2521 gacgaaggac tggacgccgc gttcaccgcc cacggggtgg atgcgatcgt cgcccccacg
     2581 ggcgcgcccg cctggctgac ggaccacgtc aacggggacc actacgtggg cggcaacacc
     2641 acgcccgctg cggtggccgg gtacccctcg gtgacggtgc cgatggggtt cgtctccgag
     2701 ctaccggtcg gtctcagctt catcggccac ccgtggaccg aggcgaccct ggccggctac
     2761 gcctacgcgt tcgagcaggc cacgaccgag cgtcgcccac cccggtacct gcccacgtcg
     2821 gaggcctgac tggacgcaac cccccgggcc ggtgtcccgc tagtgctcct cgtacttgaa
     2881 ggagatgttc acccgggccc ggtagtgcgt cacgcgtccg tcctcgatcg tgaggtcgag
     2941 ccggtccacc tcgccgaccc gcagatcccg cagggacttg gccgccgtct ccacggcgtg
     3001 cttggccgcc tcctcccacg attcggtgct ggtgccgatg acctgcgtga cccggtagat
     3061 gctctccgcc atgtcggcct cctctccgcg ctcggcgtcc atcctctccc aggggaagga
     3121 cgcgctgaag cagactagcg gaccgtgacc ggaacctgct gaactaggtt gttggccatc
     3181 ccctgcacgt tccagggctg ctcgagcggc tgcgtatcgc cgttcgcgtc ggtggctcgg
     3241 caggcgagcg tgtgctcccc gggcatggcg tcccaggtga aggaccagcc ccgccaggcc
     3301 caatcgccca cgggttcctg gagcgtggcc tcgaaccacc caccgtccac gccgacctcc
     3361 acccgctcca cggcggccgc gccgcaccag gcccggccga ccagcaccac gggaccggcg
     3421 tccaccgttc gggcacgggt gaagaagtcg gggatgccgg gcgggaccat cagggctcgg
     3481 accctgatcc tggtgaccgg ctggcccgga tcgtcctcgc tctgctggaa gcggtaggcc
     3541 tccgcctgct gatagccggc gaagggctcg gtcaccgctt cgatccgcgt caaccacttc
     3601 acgctggtca tcccgtacca gccggggacc agcaggcgga gggggaaccc gtgctggggc
     3661 gggaggggct caccgttcat ctcccaggcc agcaggacct cctcacgcat ggcctgctcc
     3721 acggagaggc tgcgtccgta gtcctgctcg acgccgccct cgatcccgtg atccaggccg
     3781 gtgaaggcga tctccaccgc gtcg
//
LOCUS       DASQQQ010000076         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_389701, whole genome shotgun sequence.
ACCESSION   DASQQQ010000076 DASQQQ010000000
VERSION     DASQQQ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389701"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /gene="rpsH"
                     /locus_tag="VE646_03270"
     CDS             <1..274
                     /gene="rpsH"
                     /locus_tag="VE646_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZD79048.1"
                     /translation="EGYIGGFEPEGEGIHQAFRVRLKYGAKRERTITGLRRISKPGRR
                     VYAQRHGLPRVMGGLGIALVSTSQGVMTDRDAARKGIGGEVLAYVW"
     gene            294..845
                     /gene="rplF"
                     /locus_tag="VE646_03275"
     CDS             294..845
                     /gene="rplF"
                     /locus_tag="VE646_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HZD79049.1"
                     /translation="MSRIGKQPIEVPSGVEVTLGDEALVTVTGPRGTLSRRLHPEMRV
                     VREDDHIRVERPSEEGFHRSLHGLTRTLIANMVEGVTKGYEKRLAIVGVGYRAAMKGR
                     DLELQVGYSHPVVVSCPEGIEFEVPTPTSIVVRGNDKEQVGEIAANIRKVRKPEPYKG
                     KGIRYENEFVRKKAGKAAKGGAV"
     gene            845..1195
                     /gene="rplR"
                     /locus_tag="VE646_03280"
     CDS             845..1195
                     /gene="rplR"
                     /locus_tag="VE646_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HZD79050.1"
                     /translation="METKVKRLARVRRHARVRRKIAGSAARPRLAVYRSNRHIYAQLI
                     DDVAATTLASASDREVQASGDKTDAAKAVGELIAKRAKEGGIERAVFDRGGRLYHGRI
                     AALADAAREEGLSI"
     gene            1219..1824
                     /gene="rpsE"
                     /locus_tag="VE646_03285"
                     /pseudo
     CDS             1219..1824
                     /gene="rpsE"
                     /locus_tag="VE646_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009199242.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            1821..>2017
                     /gene="rpmD"
                     /locus_tag="VE646_03290"
     CDS             1821..>2017
                     /gene="rpmD"
                     /locus_tag="VE646_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HZD79051.1"
                     /translation="MSRLRITQTRSVIDRPRDQKDTVRRLGLHRMHHSVLQDDRPEIR
                     GMIAKVQHLVRVEEVADGGGS"
BASE COUNT          327 a          658 c          779 g          253 t
ORIGIN      
        1 cgagggctac atcggcggct tcgagcccga gggcgagggg atccaccagg ccttccgggt
       61 ccggctgaag tacggggcca agcgcgagcg cacgatcacc ggcctgcgtc gcatctccaa
      121 gccgggccgg cgggtgtacg cgcagcgtca cgggctgccc cgggtgatgg gcggcctggg
      181 gatcgcgctg gtgtccacct cgcagggcgt catgacggac cgcgacgccg cccgcaaggg
      241 catcggcggc gaggtcctgg cctacgtgtg gtgagggagc agagggaaca gggatgagcc
      301 ggatcgggaa gcagcccatc gaggtgccga gcggggtcga ggtcacgctg ggcgacgagg
      361 ccctggtcac cgtgaccggc ccgcgcggga cgctctcgcg gcgcctgcac ccggagatgc
      421 gcgtggtccg cgaggacgac catatccggg tcgagcgccc ctccgaggag gggttccacc
      481 gcagcctgca cggcctcacc cgcaccctga tcgcgaacat ggtggagggc gtgaccaagg
      541 gctacgagaa gcgcctggcc atcgtgggcg tgggctaccg ggccgccatg aaggggcggg
      601 acctggagct ccaggtcggc tactcgcacc cggtggtggt gagctgcccc gagggcatcg
      661 agttcgaggt gcccaccccc acctccatcg tggtgcgggg caacgacaag gagcaggtcg
      721 gcgagatcgc cgccaacatc cgcaaggtcc gcaagcccga gccctacaag ggcaagggga
      781 tccggtacga gaacgagttc gtgcgcaaga aggccggcaa ggccgcgaag ggtggtgcgg
      841 tctgatggag acgaaggtca aacggctggc tcgggtccgc cgccacgccc gcgtgcgccg
      901 gaagatcgcc ggcagcgcgg cccgcccgcg cctggccgtg taccgctcga accggcacat
      961 ctacgcccag ctgatcgacg acgtggcggc gaccaccctg gcttcggcct cggatcgtga
     1021 ggtccaggcc tcgggcgaca agaccgacgc ggccaaggcc gtgggcgagc tgatcgcgaa
     1081 gcgggccaag gagggcggga tcgagcgggc ggtgttcgac cgcggcggcc gtctctacca
     1141 tggacgcatc gccgcgctgg ccgatgcggc tcgagaggaa ggactttcca tctgatgcga
     1201 cggtacgact cgtccgacca gcggagcatc tacgaggagc gggtcatcgg gatcaaccgg
     1261 gtggccaagg tcgtcaaggg cggccggcgc ttctccttcg ccgcgctcgt ggtggtgggc
     1321 gacggcgccg ggcgggtggg cgtgggctac ggcaaggcca aggaggtgcc ctcggccatc
     1381 tccaagggtg tggaggaggc caagcggaag atgttcgagg tgccgatgat gggcacctcg
     1441 atccctcacc aggtggtggg ccgcgacggc gccggtgtgg tcctgctgaa gccggccgct
     1501 ccgggcaccg gcgtgatcgc cggggggccg gtccgggccc tggtggagtg cgccgggatc
     1561 aaggacatcc tgtccaagtc catgggctcc tcgaacccca tcaacatcgt caaggcggcg
     1621 gtggaggggc tgcagcagct ccgccgcccc gagcacgtgg ccaagctgcg cggtcgcgac
     1681 gtccacgagg tggcccccaa gcccatgctg gaggcggtgg aggccgccga cgagcggctg
     1741 caggccacgc gggccgccaa gctggaggag gcctacgcgg accagtcctc gcaggggtcc
     1801 tcgtaccggg gtggtcggcg gtgagcaggc tgaggatcac ccagacccgc agcgtcatcg
     1861 accggccccg ggaccagaag gacacggtcc gccggctcgg cctgcaccgg atgcaccact
     1921 cggtgctcca ggacgaccga cccgagatcc gcggcatgat cgccaaggtg cagcacctgg
     1981 tccgggtgga ggaggtcgcc gacggcggcg ggtcgga
//
LOCUS       DASQQQ010000077         3208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_61226, whole genome shotgun sequence.
ACCESSION   DASQQQ010000077 DASQQQ010000000
VERSION     DASQQQ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61226"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..372)
                     /locus_tag="VE646_03295"
     CDS             complement(118..372)
                     /locus_tag="VE646_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79052.1"
                     /translation="MREYLARIGRAGGRKSRRTLDPETARRMVAVREARRAYRRFHTS
                     CFWSYRPDLEIGLDDVEWVAEQLTKHGDREAWRVARSLCP"
     gene            complement(426..1403)
                     /locus_tag="VE646_03300"
     CDS             complement(426..1403)
                     /locus_tag="VE646_03300"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZD79053.1"
                     /translation="MAHGQRTARYAVLAVLVLSAACTDRGSIVSPEEPATGKPPPVDP
                     GDPLFSRGGTWDARAADMDGDGMSDLLVTTHEGWRQPGGVWLQRTDGWFGSAFHFPKS
                     NDRHGCATADVNLDGLIDFYCQTGTMKGEGERANELWLQQTDGTFVDGAGAWGVKDSY
                     GRGRRPIFFDHDGDGGPGLYTTVWGKRVDDEVNRNRLWINEGGTGSTRSSWGPTTPPW
                     GSRRIRRRAVATSRSSSTAERWCSPAGMTGPARSCGEVPRRADGEKGGVFRFAPIRSC
                     ASSLPPAGLQQLLPGHSCHADRPQPRHEGCRCVLSWPPMATPRCDHGRP"
     gene            complement(1764..>3208)
                     /gene="dacB"
                     /locus_tag="VE646_03305"
     CDS             complement(1764..>3208)
                     /gene="dacB"
                     /locus_tag="VE646_03305"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HZD79054.1"
                     /translation="ACATARGGAPPPGEAIARVLAAAPLDQVHWGLLAVDAASGRILQ
                     ERASARKFVPASNMKLLVTTAALERLGPDYRFRTALWAAGTMEDGGVLDGDLVLAASG
                     DPTLSERFWPSDEAPLEALADSLLAHGIRVVRGGLVVDASAWDSTTVAGTWMVGNLPG
                     RSSATGGAFAVAEGEASIVVKGGAVPGEPASVTWTPLGEPDFVESRAETVSPDSAGDL
                     DVAYLPESRRLVVTGRVPADSTEVARVSVRDPVRQAAAALHRTLEERGVTVAGGWSVA
                     WDEDEPVGGGCRSGGVPACAGAREVAALEAPPLLEVVEGILEPSQNWMTEQLVRTLGI
                     SDSTRAGWRPGLRAVEDFMVQTVGVDSLDLSLRDGSGLSAYNLVTPRALVRLLRWVRE
                     QDWGAGYRTAMAEPGEEDSTLESRFPELQGRLYAKTGTISNVNSLTGYLVADSGREIV
                     FSLLSNGSGLPSGTVRAGMDEVVRILARGY"
BASE COUNT          457 a         1201 c         1095 g          455 t
ORIGIN      
        1 ggtcgtggct gtagcggacg gtgttgggct cgaaatgcag ggccgctccg ccggcaaggt
       61 agctgtccgg cgagcggttc cgggccagca gggccagcac ggacgcctga agggccgtca
      121 ggggcatagg gaccgtgcga cccgccaggc ctcgcggtcg ccgtgcttcg tgagctgctc
      181 ggccacccat tcgacatcgt ccaatccgat ctcgaggtcg ggccggtagg accagaagca
      241 ggaggtgtgg aagcggcgat aggcacgacg cgcttcccgc accgccacca tcctgcgggc
      301 cgtctccgga tccagcgtcc gccggctctt ccttcccccg gcgcgcccga tgcgcgcgag
      361 atactccctc accttttcgt ccatactgaa cgttagccgc ttatgttgac gcggtcaatc
      421 ggcagtcacg gccgcccgtg gtcgcatcgt ggggtggcca tcggcggcca tgacaagacg
      481 caccgacaac cttcgtggcg tggctgcggc cggtcggcat gacatgagtg acccggaagc
      541 agttgctgca ggccggccgg gggcaaggac gaggcgcacg accggatagg ggcgaagcga
      601 aacacgccac ccttctcccc gtcagctcgc cgcgggactt ctccacagga ccgggccggc
      661 ccagtcatcc ccgccggaga acaccagcgc tctgccgtcg atgacgaccg ggatgttgcc
      721 acagccctgc gacggattcg gcgcgacccc cacggcggtg tagtcgggcc ccacgaggac
      781 cttgtcgaac ccgttccacc ttcgttgatc cacagccggt tgcggttgac ctcgtcgtca
      841 actcgcttgc cccagacggt cgtgtacagg cccggcccgc cgtcgccgtc gtggtcgaag
      901 aagatcgggc gtcggccgcg accgtacgag tccttcacgc cccaggcccc ggcgccgtcg
      961 acgaacgtgc cgtcggtctg ctggagccag agctcgttgg cgcgctcccc ctcgcccttc
     1021 atcgttccgg tttgacagta gaagtcgatg agcccgtcga ggttcacatc ggccgtggca
     1081 cacccgtggc gatcgttgct cttcggaaag tggaacgctg agccgaacca gccgtcggtc
     1141 cgctggagcc agactccgcc cggctgccgc cagccctcgt gcgtggtgac caggaggtcg
     1201 ctcatgccgt cgccgtccat gtccgcggcc ctggcatccc aggtgccacc ccgggagaac
     1261 agcgggtcgc caggatcgac aggtgggggc ttgccggtcg cgggctcttc gggtgagacg
     1321 atgctcccgc gatcggtgca cgcggcggac agcacgagca ccgcgaggac ggcatatcgc
     1381 gccgtccgct ggccatgggc catggtcacc ttctcatggg gtgccggtgt gtacgtcggc
     1441 ccgccgcggg acctctccac agcaccgggc cggtcctgcg cggcccgccg gagaacaccc
     1501 gcaccatgcc gtcgaggacg gtcggggggt tgccgccggc cttccagcgg atggggagcg
     1561 aggcgccgcg gtcctgcttc tcccttatgc tgtcaggatc atctgcgtca aggcccgagc
     1621 acccggtgcc cagccggttg gaatcacgta aggtacctgg ctaccccaac ttgggggcac
     1681 gatccctcat cccgattcgc acccgttcgc atcccttcgc gcggcgccgg ggaaatgccc
     1741 gcgaaccacc gatgcgcccc gcctcagtac ccccgcgcca gaatccgcac cacctcgtcc
     1801 atgcccgccc gcaccgtccc cgacggaagc cccgatccgt tgctcaggag gctgaagacg
     1861 atctcccggc cgctgtcggc caccaggtag ccggtcagcg agttgacgtt ggagatggtg
     1921 ccggtcttgg cgtacaggcg tccctggagc tcggggaagc ggctctccag ggtggagtcc
     1981 tcctcgcccg gctcggccat ggcggtgcgg tagccggcgc cccagtcctg ctcccggacc
     2041 cagcggagca ggcgcaccag cgcccggggg gtcaccaggt tgtaggccga cagccccgag
     2101 ccgtcccgga ggctcaggtc cagcgagtcg acgcccaccg tctgcaccat gaagtcctcg
     2161 acggcccgta gacccggccg ccacccggcc cgggtcgagt cgctgatgcc cagggtgcgc
     2221 acgagctgct cggtcatcca gttctggctg ggctcgagga tgccctcgac cacttccagg
     2281 agcgggggcg cctccagggc cgccacctcg cgggcgccgg cgcatgcggg cacgcctccg
     2341 ctgcggcacc cgccgcccac gggctcgtcc tcgtcccagg cgaccgacca gcctcccgcc
     2401 accgtgacgc cccgctcctc cagggtccgg tggagcgcgg cggccgcctg ccggacgggg
     2461 tcgcgcacgg acacccgggc gacctcggtc gagtccgccg ggacacgccc ggtcacgacc
     2521 agacgacggc tctcggggag gtacgccacg tccaggtcgc cagcgctgtc cggggacacc
     2581 gtctccgccc tggactcgac gaagtcgggc tcgcccaggg gcgtccaggt caccgaggcg
     2641 ggctcgcccg gcaccgcgcc acccttcacc acgatgctgg cctcgccctc ggccacggcg
     2701 aaggcgcccc cggtggcgga ggaccgtccc ggcagattcc ccaccatcca ggtgcccgcc
     2761 accgtggtgg agtcccacgc ggaggcgtcg accaccagcc ctccccgcac cacccggatg
     2821 ccgtgggcca gcagcgagtc ggccagggct tcgagggggg cctcgtccga cggccagaac
     2881 cgctccgaca gggtgggatc gccgctcgcc gccagcacca ggtcgccgtc caggacgccg
     2941 ccgtcctcca tcgttcccgc agcccacagg gccgtgcgga agcggtagtc cgggccgagc
     3001 cgctccaggg ccgcggtggt gaccaggagc ttcatgttgg aggccgggac gaacttccgg
     3061 gcggaggccc tctcctgcag gatgcggccg ctggccgcgt ccacggccag gaggccccag
     3121 tgcacctggt cgagcggggc cgccgccagc acccgggcga tggcctcgcc cggcggaggg
     3181 gcgccgcccc gggccgtggc acaggcgg
//
LOCUS       DASQQQ010000078         4826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_389859, whole genome shotgun sequence.
ACCESSION   DASQQQ010000078 DASQQQ010000000
VERSION     DASQQQ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389859"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..682)
                     /locus_tag="VE646_03310"
     CDS             complement(29..682)
                     /locus_tag="VE646_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZD79055.1"
                     /translation="MDEGVGKATTDERKKRRSHQDPVRAGGEPEGRRPFQEVAREQLP
                     RLYGLAQRLGADDPEDLVQEGLMRAFRSYGTLRSPKAAPGWFSSILVNAYRDRLRKDA
                     RQVPEVSLDGLDEFSLYRQIAEEDPFPYSDALHQDFLGLFEAEDVHRVLATLPPHYRG
                     ALVLRYIEGHPTKYVARSLGLPLGTVLAQLHRGRKAFERALWDYAERTGLLDKETAR"
     gene            complement(697..1248)
                     /locus_tag="VE646_03315"
     CDS             complement(697..1248)
                     /locus_tag="VE646_03315"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HZD79056.1"
                     /translation="MWLDADDRKLAQDRRLLIAVLGALVALAWVVLWAWGRSPYGRLL
                     SHELLAEAGERGTSEGAALALALVPGWTLMTVAMMLPTSLPLVGLFHSLVRGRPHRAR
                     LVALLVAGYLAVWILFGAIAYAGDFALHRMVERTPWLEARPWIFGASVLVHRLSVAAG
                     SRQPAQNHCFSQVHAIGGLRRSS"
     gene            complement(1417..2793)
                     /locus_tag="VE646_03320"
     CDS             complement(1417..2793)
                     /locus_tag="VE646_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZD79057.1"
                     /translation="MTDAGRTWLRERLRGELIEPSSDGYDAARAVHNGMIDRHPAAIA
                     RCADAADVIAAVDAAREQDLLVSVRGGSHNVNGFATNDGGLVIDLSAMRGVRVDPERR
                     TARVEGGATWGDLDHAAHGFGLATTGGMVSTTGVAGLTLGGGIGHLARAHGLSCDNLL
                     SADVVTADGRFLAASQTQHPDLFWALRGGGGNFGVVTSFEFQLHPVGTVIGGPVFYEI
                     GVARGALRFYRDFIRNAPEELGAFFGFHLAPPLPFLPAERHGEPMCLIVACYAGDHDT
                     GREVVRPLLDFGPPVGHHLGPIPYPALQGAFDALFPPGLQHYWKAEFVKELSDEVIDV
                     HLTYGPRVPHVSSAVHLYPIDGAAGRVAPGDTAFSYRDARFATVIAAMYPDPGDTPAN
                     VAWVRDYHSALSAHSAGGAYVNFLMEEGQERVTASYRDNYQRLVEVKSAYDPTNLFRM
                     NQNIRPRS"
     gene            complement(3154..3597)
                     /locus_tag="VE646_03325"
     CDS             complement(3154..3597)
                     /locus_tag="VE646_03325"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HZD79058.1"
                     /translation="MRAVTERAMGLASGLSLRARVEPERLATGRRGTLIVEVGIPQGC
                     HIEPHKPSEPFLIPTELLIEPPEDMTVRAVIYPPDEERTCKWSPAVLRVYRGTVALTA
                     PIQVHTHAWPGARPLSGRVRYQGCTEAVCLPPAEQHFDATLDVVE"
     gene            complement(3581..4096)
                     /locus_tag="VE646_03330"
     CDS             complement(3581..4096)
                     /locus_tag="VE646_03330"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZD79059.1"
                     /translation="MSGQAGAATLVGSYDLGAEVPDFELPTLDGGMARLPDYRGRPVL
                     IFFTATWCPDCGAGAPHLEQESWQRYRERGLQVICIDVKEHAAVMRPFVERYEWTFPV
                     LLDDGGEVSMRFAPIKEGLPPEVAIINAHFILDTDGVVVYRDFLNMERFDPRAAHVRA
                     FLDEFLGEGRD"
BASE COUNT          736 a         1649 c         1712 g          729 t
ORIGIN      
        1 agctgcttga cggcctgggc gcacgtgatc atctggcggt ctccttgtcg agcagcccgg
       61 tacgctcggc gtagtcccac aacgcccgct cgaacgcctt ccgcccccgg tgcagctggg
      121 cgagcacggt ccccagtggg agcccaaggc tccgggccac gtacttggtt ggatggccct
      181 cgatgtaccg cagcacgagg gcgccacggt agtggggggg cagggtggcc aggacccggt
      241 gcacgtcctc ggcctcgaac aaccccagga agtcctggtg cagcgcgtcg gagtacggaa
      301 aggggtcctc ctcggcgatc tgccggtaca gggagaactc gtcgaggccg tcgaggctca
      361 cctcgggcac ctgccgggcg tccttccgca gccggtcgcg gtaggcgttc acgaggatgg
      421 agctgaacca ccccggcgcg gccttcgggg accgcagcgt gccgtaggac cggaacgccc
      481 gcatcagccc ctcctgcaca aggtcctcgg ggtcatcggc ccccaggcgc tgggccagcc
      541 catacaggcg cgggagttgc tccctggcga cctcctggaa gggccgccga ccttcgggct
      601 ccccgccggc ccggaccgga tcctggtgcg atcgccgctt cttccgctca tccgtcgtcg
      661 ccttgcccac cccctcgtcc accacgccgt cttctttcaa gacgaccgcc gaagcccgcc
      721 tattgcatgt acttgggaaa agcaatggtt ctgcgccggc tgccggcttc cagcggcgac
      781 cgacagccta tgcaccagga cgctggctcc aaagatccag ggtcgggcct ccagccacgg
      841 ggtccgctcg accattcggt ggagggcgaa gtctccggcg taggcgatcg caccgaacag
      901 gatccagacc gcaaggtagc cggcgacgag caaggcaacg agccgggcgc ggtggggccg
      961 cccacgcacc agcgagtgga acaggccgac caaggggagg ctggtgggga gcatcatggc
     1021 caccgtcatt agcgtccagc ccggcacgag cgcgagcgca agggccgcgc cctccgaggt
     1081 gccccgctcg ccggcctctg cgaggagctc gtgggacagg agacgaccgt acggggatcg
     1141 tccccaggcc cacagcacga cccaggccag cgccacgagg gcgccgagca ccgcgatcag
     1201 gagcctgcga tcctgggcca gcttgcggtc gtctgcgtcg agccacatgc gtcgttcctc
     1261 cggctggggc cgccctcccg cgggacgggt tcggtccgcg ggagggacgc gccgcgccca
     1321 gattcagccg ccccgaattt gaaggttccc tggacggcgt tgtggttctg gaggtcgatg
     1381 tcgaacccgt ggtcgcggcg ggcgggcctg gggcgatcag ctccgtggtc tgatgttctg
     1441 gttcatacgg aacaggttgg tcgggtcata ggcgctcttg acctcgacga ggcgctggta
     1501 gttgtcacgg tagctggccg tgacccgctc ctgcccctcc tccatgagga agttgacgta
     1561 ggcgccgccg gccgagtgcg cgctgagggc gctgtggtag tcgcggaccc aggcgacgtt
     1621 ggcgggggtg tccccagggt cggggtacat cgcggcgatg acggtcgcga agcgggcatc
     1681 ccggtagctg aaggcggtgt ccccaggagc cacccgcccg gcggcgccgt cgatcgggta
     1741 caggtgcacc gccgaggaga cgtgagggac gcgcggcccg taggtcaggt ggacgtcgat
     1801 cacctcgtcg ctgagctcct tgacgaactc ggccttccag tagtgctgca ggcccggcgg
     1861 gaacagcgca tcgaacgcgc cctgcagcgc cgggtagggg atgggaccca ggtggtgccc
     1921 cactggcggg ccgaagtcca gcagcggccg gacgacctcc cgccccgtgt cgtggtcgcc
     1981 cgcgtagcag gccacgatca ggcacatcgg ctcgccgtgc cgctcggcgg gcaggaacgg
     2041 cagcggcggc gccaggtgga acccgaagaa cgctcccagc tcctcgggcg cgttcctgat
     2101 gaaatcgcgg tagaaccgga gcgccccgcg cgcgacgccg atctcgtaga acaccgggcc
     2161 gccgatcacc gtgccgaccg ggtggagctg gaactcgaag gaggtcacca cgccgaagtt
     2221 gccgccgccg ccgcgcagcg cccagaacag gtccgggtgc tgcgtctggc tggcggccag
     2281 gaagcggccg tcggcggtga ccacgtcggc cgacagcagg ttgtcgcagg acaggccgtg
     2341 cgcccgggcg aggtggccga tgccgccgcc cagggtgagc ccggcgacgc cggtggtgga
     2401 caccatcccg ccggtggtgg ccaggccaaa gccgtgggcg gcgtggtcga ggtcgcccca
     2461 ggtggcgccg ccctccacgc gggcggtacg acgctcgggg tcgacccgca ccccacgcat
     2521 ggcggacagg tcgatgacca ggccgccgtc gttggtggcg aagccgttga cgttgtggct
     2581 gccgccgcgg accgagacga gcaggtcctg ctcgcgggca gcgtcgaccg cggcgatgac
     2641 gtcggcggcg tcggcgcagc gggcgatcgc cgcggggtgc cggtcgatca tgccgttgtg
     2701 gaccgctctg gcggcgtcgt agccgtcgct ggacggctcg atcagctcgc cgcgcaggcg
     2761 ctcacgcagc caggtccggc cggcatccgt caattcagac gtagttgtcg tcatggtgac
     2821 cccctttggg atgaagtggc acgatccagt cgaagtggcg gctacgagac gggggtggcc
     2881 gcaacgacca ccggctcagc agcggccggg gcggtgagcg agtcgaccgt cggcgtgaag
     2941 tagtggccac gctcgcagcg cgtcttcacg tgctcaacgg gaccctggct ggaggcgaac
     3001 gtccagcggt cgacgatctc ggcaggcgcc cggcaactgg ggtccgggca tgggatgagg
     3061 tcgggcatcg ccgtgctccc cccgtggtca tgctcggagt gctgtggagc tccggaaccg
     3121 ctgtccaggt tgtagcccat cgcttgctcc tttctactcg actacgtcca gggtcgcgtc
     3181 gaagtgctgc tcggccggcg gcaagcagac ggcctcggta cagccctggt acctcactcg
     3241 tccggagagg ggcctggctc ccggccaggc atgggtgtgg acctggatcg gcgccgtgag
     3301 cgcgacggtc ccccggtaca cgcggagcac ggccgggctc cacttgcacg tccgctcctc
     3361 gtctggcggg tagatgactg cccggacggt catgtcttcc ggcggctcga tcagcagctc
     3421 cgtggggatc aggaagggct cgcttggctt gtgcggctcg atgtggcaac cctgggggat
     3481 gccgacctcc acgatcagcg tccctcgccg gccggtggcc agccgctccg gctcgacccg
     3541 ggcccgaagc gagagcccgc tggccaggcc catcgcgcgc tcagtcacgg ccctcaccca
     3601 agaactcgtc gaggaacgcg cggacgtggg cggcccttgg gtcgaaccgc tccatgttca
     3661 ggaagtcgcg gtagacgacc acgccatcgg tatcgaggat gaagtgggcg ttgatgatgg
     3721 cgacctcggg gggcagcccc tccttgatcg gggcgaagcg catcgagacc tcgcccccgt
     3781 cgtcgagcag gaccgggaag gtccactcgt agcgctcgac gaagggccgc atgactgctg
     3841 cgtgctcctt cacgtcgatg cagatgacct ggagcccccg ctcccggtag cgctgccagc
     3901 tctcctgctc gaggtgggga gccccggcgc cacagtccgg acaccaggtg gcggtgaaga
     3961 agatcaggac cggtctgccg cggtagtcgg gaaggcgcgc catcccgccg tccagcgtgg
     4021 ggagctcgaa gtcggggacc tccgctccga ggtcgtagga ccccaccagg gtcgccgccc
     4081 cggcttggcc gctcattcgg tgtcctcctc tgccctcaag cgtctgcttc aacggttccg
     4141 acgcccatac agacgcccca gggtctggag gcattgcatg ttccagccaa tgcaataatt
     4201 tcgtcgctgc cgacgtcctg agtttccccc ggctttctcg gaggcttctg aacctggtcc
     4261 actggaccaa aggaggaagc ttcgatggtg gcaccacgca agtacccggt ggagttgcag
     4321 gagcgagcag tgcggctgtg gcgctccgag cagtcccgcc gcccgatcgc gcacctggcc
     4381 cgcgagctgg gcgtgcaccc ggaggcccta cgcaactgga tccgccagga ggagaccatc
     4441 accacgctag cggtgaaacc gcggcacgcc cgtgggcgcc cgcacttctc cgaaccgggt
     4501 cagtccccgg ccggggcctg ggcgacgcaa cctactgcac gaggcgaccg gagcccctca
     4561 agccgttgtc gttccgccag gcccacgaca cgggagactc tactgaccgt gaccatccga
     4621 tcggtggagc cggtgtcccc aacaacccac tgcaagtctt actagccgta gccgagcatc
     4681 cggcggggga ctgtcaagcg aacggtgtaa ctggtgtgga tagggctact tccttccctt
     4741 gctgacgcgg ccgtcgaagg tgatgtcgaa ggcgttgagg gcggccttcc accggttggt
     4801 ccaccgccgg cggccccggc cggtcg
//
LOCUS       DASQQQ010000079         4615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_170792, whole genome shotgun sequence.
ACCESSION   DASQQQ010000079 DASQQQ010000000
VERSION     DASQQQ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170792"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VE646_03335"
     CDS             <1..417
                     /locus_tag="VE646_03335"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HZD79060.1"
                     /translation="DPVEEVKVGTWILQSLGLRKRGLDLVACPSCGRAEVDVLGLTKQ
                     VHEELERRGIKTPIRVAVMGCVVNGPGEAREADLGIAAGKGQGFLIVNGEVKGKVPEE
                     RFVEVLVEEAERIAAEKAAAEKATAEKATAGSAGVG"
     gene            complement(414..986)
                     /locus_tag="VE646_03340"
     CDS             complement(414..986)
                     /locus_tag="VE646_03340"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HZD79061.1"
                     /translation="MILVVDHYDSFTYNLVQLVEALGYATEVTKSDAEPAAALVQRRP
                     EGVILSPGPGRPEDAGCFPDLLRLLPEQTPVLGVCLGHQAIGLACGARIDRAAPMHGK
                     ASLVFHEGQGILEGVADPFEAARYHSLVVLREDLPAELRLTAWTEDGLVMAAQHRELP
                     RFGVQFHPESILTPEGPRIVENFLALTRRR"
     gene            complement(983..2476)
                     /locus_tag="VE646_03345"
     CDS             complement(983..2476)
                     /locus_tag="VE646_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HZD79062.1"
                     /translation="MQVRPDRETFRSLERSWPLVPMWTELLADVSTPVGLFPALSAEG
                     PGVLLESVERSERWGRYSFAAGDPAAVVIADPAGVRVEEVRREGLPLRVPPSGTPARQ
                     ALRAIATSLSAPRLPSLPSLTGGLMGYLAYEAAGLLDGHPTPDGTRAPCPPIGLLVVD
                     RAVVFDHWRQRLLLVAHVPAGGYEAGVAALEELAATVRTASPPPPEPYREGVAPPAAH
                     GRANMPDRRYRQIVASFQEHILAGDIYQAVPSRRVSFPASHGGYPIYRRLRVSNPAPY
                     MFFVRMLGMELAGSSPEPLVRVEGRRVWTRPIAGTRPRGRTEVRDRLLEHELLADPKE
                     QAEHAMLVDLARNDLGRVCRPGTVRPTELMVVERFSKVMHIVSTVEGELEEGLDPFDA
                     LAVTFPAGTVTGAPKRRAMELISEAEPTARGPYAGAVGYRTFAGDLDFCITIRTAVVA
                     DGLAHVQTGAGVVADSDPETELRETKAKAAALLPAVAGGAEGEEVPR"
     gene            2729..4306
                     /locus_tag="VE646_03350"
     CDS             2729..4306
                     /locus_tag="VE646_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79063.1"
                     /translation="MHRRWPLRMIPAATLAVVAMLAPQPALAAQGRADGPRARSFERS
                     PSILPGVHPLPGSAGSDREGPVERSGAATALAPVSQFHFDAVPRIGPNWPADPSGGMG
                     EHWFFTAGNTSYALFDLGGAPKVGPAPFDTLFDYPARTQVYDPKVVYDPYGQTFVLVY
                     LAVNDGLRRSWIQLVTIPDTPDAEDVGTWCTRRINGDQVAGNGRQWADYPGLGFDADR
                     VVVSTNQFDFGRFRFRYAQILSFPKAGLYDCDASALPDVFARSNTRNPDGTQAFTIQP
                     ATTAGGPTARQLFLSYERGRDTSALVVWALKERASGLTLQRAAIPVGRVAISPYGTQG
                     GGGLKRSNTWWDPGDLRLINAFYDPERNRLYAAHTIYRDVKPDALRAGYGEAAIRWYE
                     VVPKGRIKASSITRRGTVGRAETDAGWPVVATDGEGNIWITYSRASAPRHEFLSAWAA
                     EIRPGSRRATVQLLAAGQARFEATPGPERWGDFNGIARDPTDPSRIVMVNQYAKSDGS
                     PPTRDWQETVDVVVDGG"
     gene            4351..>4615
                     /locus_tag="VE646_03355"
     CDS             4351..>4615
                     /locus_tag="VE646_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HZD79064.1"
                     /translation="MRLSQLFAPTLREDPAEAEIASHRLLLRAAFIRQVAAGVYTTLP
                     LGLRSMRKIERVVREEMDASGAQEIRMPIVLPSEPWRATGRWDL"
BASE COUNT          630 a         1709 c         1693 g          583 t
ORIGIN      
        1 gacccggtgg aagaggtgaa ggtggggacc tggatcctgc agtccctggg gctgcgcaag
       61 cgcgggctgg acctggtggc ctgcccctcg tgcgggcggg ccgaggtcga cgtcctgggt
      121 ctcaccaagc aggtgcacga ggagctggag cggcgcggca tcaagacccc gatccgggtg
      181 gccgtcatgg gctgcgtggt gaacgggccc ggcgaggccc gcgaggccga cctgggcatc
      241 gcggccggca agggccaggg cttcctgatc gtgaacggcg aggtgaaggg caaggtcccc
      301 gaggaacgct tcgtcgaggt cctggtggag gaggccgagc gcatcgccgc cgagaaggcc
      361 gccgccgaga aggccacggc cgagaaggcc accgccgggt ccgccggcgt cggctagcgc
      421 cggcgcgtca gggccaggaa gttctccacg atcctgggac cttccggggt gaggatcgac
      481 tccgggtgga actgcacccc gaaccgcggg agctcacggt gctgcgcggc catcaccagc
      541 ccgtcctcgg tccaggcggt gaggcgcagt tcggccggga gatcctcgcg cagcaccacc
      601 agcgagtggt accgagcggc ctcgaaggga tcggccaccc cctccaggat cccctggccc
      661 tcgtggaaca ccagcgaggc cttcccgtgc atcggcgccg cccgatcgat cctggccccg
      721 caggccaggc cgatggcctg gtgccccagg cagaccccca gcaccggcgt ctgctcgggg
      781 agcagccgca gcaggtcggg gaagcagccg gcgtcctccg gtcggcccgg ccccggcgag
      841 aggatcacgc cctcgggacg ccgctgcacc agcgcggccg ccggctcggc gtcgctcttc
      901 gtgacctccg tggcgtagcc gagggcctcc accagctgca ccaggttgta ggtgaacgag
      961 tcgtagtggt ccaccaccag gatcaccgcg ggacctcctc accctccgcg ccgccggcca
     1021 ccgccggcag cagtgcggcg gccttggcct tggtttcccg cagctcggtc tcggggtccg
     1081 agtccgccac caccccggcc ccggtctgca cgtgggccag cccgtcggcc accaccgcgg
     1141 tccggatcgt gatgcagaag tccaggtccc cggcgaaggt ccggtacccc acggcgcccg
     1201 cgtagggccc ccgcgccgtg ggctccgcct ccgagatcag ctccatggcc cggcgcttcg
     1261 gcgcgccggt cacggtcccg gcggggaagg tcaccgccag ggcgtcgaac gggtccagcc
     1321 cctcctccag ctccccttcc accgtgctga cgatgtgcat caccttcgag aaccgctcga
     1381 ccaccatgag ctcggtgggg cggaccgtgc ccgggcggca cacccgaccc aggtcgttcc
     1441 gggccaggtc caccagcatc gcgtgctcgg cctgctcctt ggggtcggcc aggagctcgt
     1501 gctccagcag gcggtcccgg acctcggtcc gcccccgggg ccgcgtgccg gcgatggggc
     1561 gggtccagac ccggcggccc tccacccgca ccagcggctc gggggaggag ccggccagct
     1621 ccatgccgag catccggacg aagaacatgt agggcgccgg attcgagacg cgcagccgcc
     1681 ggtagatcgg gtagccaccg tgggaggcgg ggaaggacac gcggcgcgag ggcaccgcct
     1741 ggtagatgtc gccggccagg atgtgctcct ggaacgaggc cacgatctgc cggtagcgcc
     1801 ggtcgggcat gttggcccgg ccgtgggcgg ccggtggggc cacgccctcc cggtacggct
     1861 cggggggcgg cggggacgcc gtgcggaccg tggccgcgag ctcctcgagg gcggcgaccc
     1921 ccgcctcgta cccgcccgcc ggcacgtgcg cgaccagcag cagccgttgg cgccagtggt
     1981 cgaagaccac cgcccgatcg accaccagca ggccgatcgg ggggcagggc gctcgggtgc
     2041 cgtccggggt ggggtggccg tccaggagcc cggcggcctc gtacgccagg tagcccatga
     2101 ggccaccggt gagcgagggc aaggagggca gccgcggcgc ggacagggag gtggcgatgg
     2161 cccggagcgc ctggcgcgcc ggcgtccccg agggcgggac ccgcagcggc agcccctccc
     2221 ggcggacctc ctccaccctg accccggcgg ggtcggcgat caccaccgcg gccgggtccc
     2281 cggcggcgaa ggagtaccgg ccccaccgct ccgatcgctc cacgctctcg agcaggaccc
     2341 cgggcccctc ggccgacagc gcggggaaca ggcccaccgg ggtggagacg tcggccagca
     2401 gctcggtcca catcgggacc aggggccagc tccgctccag ggagcggaac gtctcgcggt
     2461 cgggtcgaac ctgcaaccgg acctcctgcg gacatcacaa ggccccgggt cctggcccgg
     2521 ggccgttggc gggatcgccg cggtggtgct caggcgcgcg ccggctccgg gctcctcgag
     2581 ccccggggcc aggaccagcc gcacgcaccg cgcctcatgg tccgccgatt gtcgggcctc
     2641 gggcccgccc ccgtcaacgc cgtggacgtc gttgcccgtt tccgatacgg tctggccggc
     2701 atcggcccgc cgcccaggag aaccaaccat gcatcgacgc tggcccctcc gcatgatccc
     2761 ggcggcgacc ctggcggtgg tggcgatgct ggcgccgcag ccggcgctgg ccgcccaggg
     2821 ccgggccgac ggaccgcggg cacgctcctt cgaacggtcg ccctcgatcc tgcccggggt
     2881 ccatccgctc ccgggctcgg cgggctcgga ccgcgagggg ccggtggagc ggtccggcgc
     2941 agccaccgcc ctcgccccgg tctcgcagtt ccacttcgac gcggtgcctc ggatcggacc
     3001 gaactggccg gccgacccgt ccgggggcat gggggagcac tggttcttca ccgccggcaa
     3061 cacctcctac gccctgttcg atctgggcgg ggcgccgaag gtcggtcccg cccccttcga
     3121 cacgctgttc gactatccgg cccggaccca ggtgtacgac cccaaggtgg tctacgaccc
     3181 gtacgggcag accttcgtgc tggtctacct ggcggtgaac gacgggctcc ggcgctcctg
     3241 gatccagctc gtcacgatcc cggacacccc cgacgccgag gacgtcggca cctggtgcac
     3301 ccggcggatc aacggcgacc aggtggccgg caacggacgg cagtgggccg actacccggg
     3361 gctgggcttc gacgccgacc gggtggtggt cagcacgaac cagttcgact tcggccggtt
     3421 caggttccgc tacgcgcaga tcctgtcctt ccccaaggcc ggcctgtacg actgcgacgc
     3481 ctccgccttg cccgacgtct tcgcgcgctc gaacacgcgg aacccggacg ggacccaggc
     3541 cttcacgatc cagccggcca ccacggccgg cggtcccacc gccaggcagc tgttcctgtc
     3601 ctacgagcgg gggcgcgaca cctccgcgct ggtggtatgg gcgctgaagg agcgggcctc
     3661 ggggttgacg ctgcagcggg cggcgatccc cgtgggccgc gtcgcgatct cgccctacgg
     3721 gacgcagggg ggcggcggcc tcaagcgctc caacacctgg tgggacccgg gggatctccg
     3781 cctgatcaac gccttctacg acccggagcg caaccgcctg tacgcggccc acaccatcta
     3841 ccgggacgtg aagccggacg ctctgcgcgc cggctacggg gaggccgcga tccgctggta
     3901 cgaggtggtg cccaagggcc ggatcaaggc ctcgagcatc acccgcaggg gcaccgtggg
     3961 tcgggccgag accgacgccg ggtggccggt ggtggccacc gacggcgagg ggaacatctg
     4021 gatcacctac agccgggcca gcgctcctcg gcacgagttc ctctcggcct gggcggccga
     4081 aatccggccc gggtcgaggc gggccacggt ccagctcctg gccgcgggcc aggcgcggtt
     4141 cgaggccacc ccaggaccgg agcgttgggg cgacttcaac gggatcgccc gcgaccccac
     4201 cgatcccagc cggatcgtca tggtgaacca gtacgcgaag agcgacggtt cgcctcccac
     4261 ccgggactgg caggagacgg tcgacgtggt cgttgacggc ggctgatccg ggccggccgg
     4321 gcggcgccgg ggcggtatcc tgtgtgcccc atgaggctgt cccagctgtt cgcccccacc
     4381 ctgcgcgagg accccgccga ggccgagatc gcctcgcacc gcttgctgct gcgggccgcg
     4441 ttcatccggc aggtcgcggc cggcgtgtac accacgctgc cgctcgggct gcggagcatg
     4501 cggaagatcg agcgggtggt gcgcgaggag atggatgcct cgggcgccca ggagatccgg
     4561 atgccgatcg tgctgccgtc ggagccctgg cgggccaccg gccggtggga cctgt
//
LOCUS       DASQQQ010000080         3298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_61274, whole genome shotgun sequence.
ACCESSION   DASQQQ010000080 DASQQQ010000000
VERSION     DASQQQ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61274"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..395)
                     /locus_tag="VE646_03360"
     CDS             complement(201..395)
                     /locus_tag="VE646_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020470429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HZD79065.1"
                     /translation="MLLLWDFTANYIRARFGQDERGASLVEYALLVALIAVVCIVAIA
                     FLGRAASSKFSSVGGSINGA"
     gene            complement(530..1186)
                     /locus_tag="VE646_03365"
     CDS             complement(530..1186)
                     /locus_tag="VE646_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD79066.1"
                     /translation="MSIRVLLADDHRMLREGLRRSLEEQGFDVVGEAGDGEEALTAAQ
                     ELQPAVVLMDVTMPVLDGVEATRQIRERAPDVRIVMLTMHADAEVVAQAIRAGASGYL
                     TKDCSIEEVTDAIRLAASGETVLSPDLAATMLNEVRKLAQEEAREEPARQEIITKREE
                     EVLQAIADGLSPAEVAKQLYISQKTVKNHLASIYQKLDARDRTQAVVRAVRMGIIRLD
                     "
     gene            complement(1183..2694)
                     /locus_tag="VE646_03370"
     CDS             complement(1183..2694)
                     /locus_tag="VE646_03370"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HZD79067.1"
                     /translation="MGGLVLVAYATWRSFRPIRFTDETGAFLAVLLEVGVHTLVVAGT
                     GYWASPYVFSLLTAITVAGFARGFNLAFLVGGASALAVAVPYHVVLDIPTRVALRESV
                     QWTVELLMVAAVAGYSRRFWGEAEERHLLALDRLGRLTEANNLLYSLHRLAQALPASL
                     DLEEVLESSLGRLRTLVDATALAIFLPDETSTRWLVARQQGTRLPPVLEADLLPPPVA
                     DATRGRAAVTIEDLQVPSGRGMSIRSRSGIYAPLTARGALVGVLAVEHDEVEHFNTHD
                     IDVFNGFVEPVGLAIDNARWFQRLRTVSADEERTRIARDLHDRVGQSLAYLTFELERL
                     QRHAESEEAKENLGQLRDDLRTVVGDVRETLYDLRTDVSETDDLPTILGQFLDRVQAR
                     TEIEVLTSYQAKARLPLPQEREMWRVAQEAVTNAERHADASIIRVSWRCDAKKAELVV
                     ADNGRGFERGKAGRMDSYGMLGMRERAASVGASLDVDSEPGQGTTVRCTLRRV"
     gene            complement(2820..>3298)
                     /locus_tag="VE646_03375"
     CDS             complement(2820..>3298)
                     /locus_tag="VE646_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZD79068.1"
                     /translation="SWLKRKVEERKDAIRRDLPDTLDLLAISVEAGSGFEGAMQVVCQ
                     HFDSPLAEEFSRTLKEMELGLSRREALQNLKRRTEVPELSNFVLTLTQADALGIPVGR
                     VLKTQAAEMRLRRRQWAREKAGKLPVKILFPLVIFIFPVIMIVILGPAAVEISGAL"
BASE COUNT          486 a         1138 c         1171 g          503 t
ORIGIN      
        1 gcagtgaagt cccagagaag aagcatggct cccgagggtc cctttccttg tcgtgacgtg
       61 cccccgtggg ggcctgctgt cacagcaagt atcggcgccg aggcgggcgc atacatgggg
      121 cgatcggatc attgccggac acgcgaagag gggcgggctc gggagcccgc ccctctcggg
      181 gagaatctac ggtcgtctga tcaggcgccg ttgatggagc cgccgaccga gctgaacttg
      241 gacgaggcgg cccggcccag gaaggcgatg gccacgatgc agaccacggc gatcagggcg
      301 acgaggaggg cgtactcgac cagcgaggca ccccgctcat cctgaccgaa acgggcccgg
      361 atgtagtttg cagtgaagtc ccagagaaga agcatggctc ccgagggtcc ctttccttgt
      421 cacgacgtgc ccccggtggg ggcctgctgt cgttggaagt atcggtgtga ccggtgccgg
      481 tctccatggg gcgttcggct catttccggc cacacccggt ggcgggagct caatccagcc
      541 ggatgatgcc catgcggacg gcccgcacga ccgcctgggt gcgatcgcgg gcgtcgagct
      601 tctggtagat cgacgccagg tggttcttca cggtcttctg gctgatgtag agctgcttgg
      661 ccacctcggc gggggagagg ccgtcggcga tcgcctggag gacctcctcc tcacgtttgg
      721 tgatgatctc ctgccgggcc ggctcctccc gggcctcctc ctgggcgagc ttgcggacct
      781 cgttcagcat cgtggccgcc aggtcgggtg acagcacggt ctcgccgctg gccgccagcc
      841 ggatggcgtc ggtgacctcc tcgatggagc agtccttggt gaggtagccg gacgcgcccg
      901 cccggatggc ctgggccacc acctcggcgt cggcgtgcat ggtgagcatg acgatccgaa
      961 cgtctggtgc ccgctcgcgg atctgccggg tggcctccac tccgtcgagc acgggcatgg
     1021 tgacgtccat cagcaccacc gcgggctgga gctcctgggc ggcggtgagc gcctcctcgc
     1081 cgtcgcccgc ctccccgacc acgtcgaagc cctgctcctc gagcgagcgc cgcagcccct
     1141 cgcggagcat gcggtggtcg tcggcgagga gaacgcggat gctcataccc gtcggagggt
     1201 gcagcgcacg gtggtgccct gccccggttc cgaatcgacg tcgagcgacg cgccgacgct
     1261 cgccgcccgc tctctcatcc ccagcatccc gtaggagtcc atgcggcccg ctttccctct
     1321 ctcgaagccc ctgccgttgt ccgccacgac gagctcggcc ttcttggcgt cgcaccgcca
     1381 ggacacccgg atgatagagg cgtcggcgtg ccgttccgcg ttggtgaccg cctcctgggc
     1441 gacccgccac atctcccgct cctgggggag aggcagccgg gccttcgcct ggtaactggt
     1501 gaggacctcg atctcggtcc gggcctgcac ccggtcgagg aactgcccga ggatcgtggg
     1561 gagatcgtcg gtctcggaca cgtcggtgcg caggtcgtag agcgtctccc gcacgtcgcc
     1621 gacgaccgtg cgcaggtcgt cgcggagctg gcccaggttc tccttggcct cctcgctctc
     1681 ggcgtggcgc tgcagccgct cgagctcgaa ggtcaggtag gcgagcgact ggcccacccg
     1741 gtcgtgcagg tcgcgggcga tgcgcgtccg ctcctcgtcg gcgctcaccg tacgcagccg
     1801 ctggaaccag cgggcgttgt cgatggcgag cccgaccggt tccacgaagc cgttgaacac
     1861 gtcgatgtcg tgggtgttga agtgctcgac ctcgtcgtgc tccaccgcca gcaccccgac
     1921 cagcgccccg cgggcggtga ggggggcgta gatacccgac cgcgaccgga tggacatgcc
     1981 ccgaccgctg ggtacctgga ggtcctcgat cgtgacggcg gcccgccccc tggtggcgtc
     2041 ggcgaccggc gggggcagca ggtcggcttc cagtacgggg ggcagcctgg tgccctgctg
     2101 gcgggcgacc agccagcggg tcgaggtctc gtcggggagg aagatggcca gcgcggtggc
     2161 gtcgaccagc gtgcggagcc ggcccaggct ggactcgagc acctcctcca ggtcgagcga
     2221 cgccggcagc gcctgggcga ggcggtgcag cgagtacagc aggttgttgg cctcggtgag
     2281 ccggcccagc cggtcgagcg ccagcaggtg gcgttcctcg gcctcgcccc agaagcggcg
     2341 ggagtacccg gcgaccgcgg ccaccatcag cagctcgacc gtccactgca ccgactcccg
     2401 tagggcgacg cgggtgggga tgtcgagcac cacgtggtac ggcaccgcga cggccagcgc
     2461 cgacgccccg cccaccagga aggccaggtt gaagccccgg gcgaacccgg cgacggtgat
     2521 cgcggtgagc agggagaaga cgtagggcga agcccagtac cccgtgcccg cgacgaccag
     2581 cgtgtgcacg cccacctcga gcagcacggc gaggaacgcg ccggtctcgt cggtgaagcg
     2641 gatcggccgg aagctccgcc aggtggcgta ggcgacgagc accagcccgc ccacgaggta
     2701 ctcgacctcg tcgcccttgg gcagcggcag ggccagggcg gcgccgacgc cgatcgtgag
     2761 ccagcggatg gcgaggatgg gcggccggaa cagggccgcc cggtcgcccg cctcgccggc
     2821 tagagcgcgc cgcttatctc gacggcggcc ggcccgagga tcacgatcat gatcaccggg
     2881 aagatgaaga tcaccagcgg gaacaggatc ttcaccggca gcttgccggc cttctcccgg
     2941 gcccattgcc ggcgccgcag ccgcatctcc gcggcctggg tcttcagcac ccggcccacg
     3001 gggatgccca gggcgtccgc ctgcgtgaga gtcagaacga agttcgatag ttcaggtacc
     3061 tcggtccgtc gcttgaggtt ctgaagggcc tcccgccggg agagccccaa ctccatctcc
     3121 ttcagcgtac gggagaactc ctcggcgagc ggggagtcga agtgctggca caccacctgc
     3181 atcgctccct cgaagccgct gccggcctcc accgagatgg ccagcaggtc gagcgtatcg
     3241 ggtaggtccc ggcggatggc gtccttgcgc tcctcgacct tgcgcttgag ccacgact
//
LOCUS       DASQQQ010000081        14410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_254444, whole genome shotgun sequence.
ACCESSION   DASQQQ010000081 DASQQQ010000000
VERSION     DASQQQ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14410
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254444"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VE646_03380"
     CDS             <1..197
                     /locus_tag="VE646_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018208307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HZD79069.1"
                     /translation="AAGACERRARVEGVRGKNLTPFLLRCLSERTDGRSLEANLALLE
                     SNAALAAEVAVALAEERSLT"
     gene            complement(190..1236)
                     /locus_tag="VE646_03385"
     CDS             complement(190..1236)
                     /locus_tag="VE646_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZD79070.1"
                     /translation="MIVLRIIGALAAVVLFGGAVVGHQRRQVSRLTLIVTALISVGVV
                     LLAIAPNLFNPVFDILNFRPGSGRRLTAVLLIAVIIMAGLLLRMQGAVDSSERSIRLL
                     VESLGQSTFDWEQAHALPPGPRIVVLSPAFNEAENVPAVIHAMPDEIEGYRVIPIVID
                     DCSDDGTAGAARTAGALTARLPIRRGGGLALRVGYDLALKLGADIVVSIDADGQHQPE
                     ELPTLVKPIIEGTADHVNGSRMLGDFERGALIRHLGVHFFSWLVTILTGQRVTDISSG
                     YRATSAETLRKLLLEQDQFWTSEVTIEALRHHARIVEVPVTFLTRRGGVSKKPKSLRY
                     AWNFSKAIGKTWLR"
     gene            complement(1268..2446)
                     /locus_tag="VE646_03390"
     CDS             complement(1268..2446)
                     /locus_tag="VE646_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017314734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZD79071.1"
                     /translation="MRILILGGDGYLGWSTSMYFSRRGHQVHAVDNYLRRRAHDEAGT
                     DSLTPILEDLPARAAAWKDATGLEIGVTEGDLLDWETVERVFREFEPEAIVHYGEMPS
                     APYSMIDREHTVFTQTNNVMGTLNVLYAMAEYAPNAHLVKLGTMGEYGTPNIDIEEGF
                     ITIDHNGRSDTLPYPKMPGSFYHLTKVHDSHNIHFACRVWGLRSTDLNQGVVYGIETE
                     ETKLDPRLVTRFDYDEVFGTALNRFCLQAVIGHPLTAYGKGGQTRGYLNIVDTLRCVE
                     LAVENPPAEGEYRVFNQFTEQFSIMELAHKVVEAGQHHGLRVGIDHVENPRVEAEEHY
                     YNAKHTKLLDLGLEPHLLSETLIESEIAAIERLKDRVILDHIMPRTRWRAPLHEPTAT
                     "
     gene            complement(2434..4362)
                     /locus_tag="VE646_03395"
     CDS             complement(2434..4362)
                     /locus_tag="VE646_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79072.1"
                     /translation="MVVGIALGAAAAAIIAFYLAPFGAHAPRVPSGFDTPRYIWRSNI
                     VTDHGFRALAEIHVPNQDPLAERAAYPIFAALVHATSGITPLRLAFVLPAVMAVTIGL
                     AAAGFALRGLGDPPWTAPLYALGVAFSVDLARTAIGHGDNLLVDALLVVAALAVVLAT
                     KDRLSLIVPVTLLAAALATHWIFAGEFSALVAALALVEIPSSIRLHRAGAPARRTPSG
                     RLGLVLAGAGLSAVGLLLVLGLAHIRIPDITAATLEGKFERLLGPSDLILALLAIGIG
                     VFWLWTTREGLRRTSVALMALWAITVPAAMLAYRTLHLSVPAYRVVGSALGVPVLVLA
                     GLVAGGRCLARASRRWTAVPWGRLVTASIFGLGALGILGLILPGWHAWEQRAPAFTIR
                     QASEAAAVGQYLERFPDRPAIVVVSGPSRVKQDPVLRSLLPTDQIARPHLFVGRSADL
                     LEGHPTIREGNSITAASEATFREVRPLLEQHPIILALASFGRPVSDTGPGVPIAPGVT
                     LVEGPPPTGPIRPAPFPSPSDATRIMDAAFVLVLLFGVGIGWSRVLRGRSFDIARVAL
                     SPGLGLAALVLVGVVGGSLGLDLGGAPGIVLVLVTAAAGWIPSSSRGAGPDRGTAAMS
                     GTIPPRPTVRRKGLHCAS"
     gene            complement(4619..5869)
                     /locus_tag="VE646_03400"
     CDS             complement(4619..5869)
                     /locus_tag="VE646_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015195388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HZD79073.1"
                     /translation="MTVPDLSPLRARFPALTRQIDGRPCAFLDAPGGSQVPDVVIEAM
                     ASYLRTSNANSHGAFVTSEETDAIVDQAHRAAADLVGSEPDQAVFGPNATTLLFAVSR
                     SIGRTLGPGDEVVVTRLDHDANVRPWVMAAEDAGATVRWVDLDEEHVTLDLGSFDAAV
                     GARTKVVAFTLASNAVGTVTPAAELVRRVRERSDALVVCDAVHYAQHRLIDVGELGCD
                     LLACSPYKIFGPHLGILFGRRDLLSELRPYKVRPAEEHLPFRWETGTQNHEGLAGFGA
                     AVDYLAGIGRTYGSPAREGRRPALEAAFQAIGSHESELARRFLDGVRSVPRLRLYGIA
                     DPARLAERTPTFAVRLEGQHPADTAKALGEQGIFTWDGNYYALELMERLGLEEDGGAL
                     RIGFCHYNTTEEVDRVLQALSLLA"
     gene            complement(5928..6560)
                     /locus_tag="VE646_03405"
     CDS             complement(5928..6560)
                     /locus_tag="VE646_03405"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HZD79074.1"
                     /translation="MSAGPPDDRFEPGRAVALREIWDGRVWSAIPARVVEDRSDLRLL
                     FVHPGTVWKYPRAPDGRWLKLPEPRWVLADRTWDPPRAILSFALPGRSYAPMLFWDRD
                     WEPLHWYVNLESPLRRTPVGFDYTDHVLDVLLPPDRSSWSWKDEDELDQALRRGLFSP
                     RQAQGFRRDGEEAVRLLKDRLVPFDHDWRDWRPPPEWAAPELPADWDRVD"
     gene            complement(6557..8122)
                     /gene="ftcD"
                     /locus_tag="VE646_03410"
     CDS             complement(6557..8122)
                     /gene="ftcD"
                     /locus_tag="VE646_03410"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HZD79075.1"
                     /translation="MGTELEVSVRDNGVLIAAVPNFSEGRRQDVIDEICRALEQPGAH
                     LVYRQADPDHNRLDCTVIGSPRAVSRSALGAAAVAVRLIDMDRHRGNHPRMGAVDVIP
                     FMPVRDITMDDCVAFARAFAQELAERLDLPVYCYDRAALSEGRRSLAEVRRGEYEELR
                     AAVERGERLPDFGPARIGPAGATAVGARKALIAFNVYLSGGDQAAAKDVARAVRESSG
                     GLPAVRAIGFDVPQRDCVTVSMNLVDHEVTGLRAAFRAVRDEARRRGLEVRSSEIVGL
                     VPQAAISDADIEELRLEGFDADAQILERLVSAAEASSGIGSRRIGDFLDVLSSDRPTP
                     GGGAAAGLFGATGASLISMVARLTVDKEGYESAWEPMREALGRAEEDRRAFLDLADRD
                     AAAFEAVMTAFRLPKETEEQIDERRQAIQSAFLGAALVPLEIARRAVDLIDMAANAVR
                     IGNQNAASDGASAAEALYAAARCGIYNVEINVASLKDQAKSAELREEVDSLRARARTL
                     LDQVEAAFAERMG"
     gene            8313..8564
                     /locus_tag="VE646_03415"
     CDS             8313..8564
                     /locus_tag="VE646_03415"
                     /inference="COORDINATES: protein motif:HMM:NF039800.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HZD79076.1"
                     /translation="MSKRYDEVVEVLVEADAGMPSAFRWRGRRYEVADVIGRWRIEGR
                     WWADGRDREYWRVEARGGAVWDLYHDRLGGRWHLERLWD"
     gene            8585..9700
                     /locus_tag="VE646_03420"
     CDS             8585..9700
                     /locus_tag="VE646_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZD79077.1"
                     /translation="MDPVESARAFARQIRSYIDDWELEATYPRPVVASTGLTGLYAPG
                     ELGGLGLSYTEAVEVFEELGRADAAIAFSLSMHNAVTAIVADAGDDGLRHRWAKRLIR
                     GEALGGFSLTEPHAGSDATAITTRAVQTQVGWRITGSKAWVSLAGEADLFLVVCKTSD
                     QPGHRDIAVLAVQREGGGVSFPRIYRKPRAAFLPIGEMELNDAPATLVAPPRTGMQAA
                     LGAIDVARCMIGAIATGMHAEALDLALRYARDRKLFGGRVIDQQGLQWSLADAETDLV
                     AGRLLTRRAAEALGSAEGTVAVAHAKRFCPDAALRAVVLASQILGAYGWLENHPLVRF
                     MDLAKMLQTVDGTTEVQRVIIARDLLKRAEELGPEPS"
     gene            9757..13119
                     /locus_tag="VE646_03425"
     CDS             9757..13119
                     /locus_tag="VE646_03425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZD79078.1"
                     /translation="MATTSPGFVHLDARSFFSLKDGAFSPEDLAGRAAELGMPAVALT
                     DRDGLYGAARFVAACAREGVQPILGATLTVRDVLGGGRGGPSPSTTVILLAQDQRGYA
                     NLCRLLTDAHMAGERGEPSLAPEQICAHPEGLLALLGPDSLPGGLAVAGRFDAARRAA
                     EPYREAFGGRLWVAVQHRLEANSAAEVRMLLRFAEWIAARTVATNPVRYLVPQDAFLA
                     DALECMRQIVPVNHRNVSRTNAEGYLKAPAEMRELFRERPDLADRTLELTESCTFDLG
                     LGQVHFPAFPTPAGRSAASVLAERCWRGMERRGLETTRRVRDRLDHELGMIRRMGFAA
                     YFLNVADIVADIKAMGIRCACRGSAAGSLVCHLTGISDVDPVRHDLAFERFMNPLRDE
                     LPDIDVDVESARREDVYDMILSRYGQDRCACVAMVDTYRARSAIREVGKALGFPEVEV
                     DTVAKAFPHIGAGDIRKAIEILPELAGSNMNAGQLGLLFRVAERLNGFPRHLALHPSG
                     LVISNDDLVRRVPLERSFQGYRMIQADKDDVELLGYLKLDVLGVRMLSSMRHALDEIA
                     RTEAVKVDLDRIPLDDEPTFELIRASDTLGCFQIESPGQRELLQKLQPTRWEDLIVDI
                     SLFRPGPVKSDMIGPFLRRRAGMERPVYLHPSFRPALQETFGVIVYHEQVIRTVAAVT
                     GCDPSEADRVRRHLSDDLEVGRIRGWFLERAKSNLALSDRDAERIWREVWSFASFGFC
                     KAHAAAFAVPTYQSAWLKTHYPAHFLAGVLTHDPGMYPRRLILEDAREHGIAILPLDV
                     NRSERDYVVEPSLSAARAVGSGTTATTAPINTYGIRLGLKDVHGISDAEMRSILEARA
                     DRPFRDVGDFLRRTRVARPVVEAIAHAGGFDALPGGTRRDRLYEAMVTEPQREGDQLT
                     LDLWSATGSVTGLATVAVQRDAGAPGNQRGSQPHSLPEYTDAERVRAELEVLGLDASR
                     HLISFFEPLLADLGVVRSCDLWRHRSEAWVMVAGVKVASQTPAVRSGQRIVFLTLDDG
                     TGPVDVTVFEKVQPKVVKTVFHASVLAVWGRLRRTGVKGVSIIAEDVWDLVALQGARR
                     EGRLERSLAEHASSPAPSPRKFWHASGGSAGW"
     gene            13182..14387
                     /gene="dinB"
                     /locus_tag="VE646_03430"
     CDS             13182..14387
                     /gene="dinB"
                     /locus_tag="VE646_03430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZD79079.1"
                     /translation="MGPPRAAEAILHVDLDAFYASVEVQKDPSLAGKPVVVGGAGTRG
                     VVMSASYEARAFGVRSAMPAVRARRLCPDAVFLPPDFESYRAHSNRFREILLSFTPLV
                     EPIALDEAFLDVAGAELLFGPPRRIAELIRGRVRTEVGVSCSVGVAPNKLLAKLASRR
                     AKPDGLVVVPSDGVAAFLEPLPATELWGVGEKTMEVLSRLGARTVGDLGRIPPGVLGR
                     LIGERHAADLRDLARGRDERAVMPYEAPKSVSHEETFAVDLDAEEDLLREALALSHKV
                     GSRLREDGYRARTVVLKVRLANFTTLTRSRTLPGATDIGAEIFDVARDLYRGLPGAHR
                     RVRLLGVQATGLVPAGAEQLALLRGERWGDVERAVDRIERRFGRGAARPASLMDRSRG
                     RSPGEQVGR"
BASE COUNT         2068 a         5048 c         5199 g         2095 t
ORIGIN      
        1 ccgcggcggg ggcctgtgag cgccgtgctc gggtcgaagg ggtgcggggc aagaacctca
       61 cgccgttcct gctccgatgc ctgtccgagc ggacggacgg ccgcagcctg gaggccaacc
      121 tggccctgct ggagtccaac gcggcgctcg cggccgaggt cgcggtggcc ctggccgagg
      181 agcgctccct cacctgagcc aggtcttgcc gatcgccttc gagaagttcc acgcgtagcg
      241 aagggacttc ggcttcttgg agaccccacc tcgacgggtg aggaaggtga ccggcacctc
      301 cacgatccgc gcgtggtgac gcagggcctc gatcgtgacc tccgaggtcc agaactggtc
      361 ctgctccaac aggagttttc ggagggtctc ggcgctcgtg gctcggtacc cgctggagat
      421 gtcggtgacc cgttgcccgg tgaggatcgt gaccagccag gagaagaaat gcacccccag
      481 gtggcggatc agggctccgc gctcgaagtc ccccagcatg cgggacccgt tcacgtggtc
      541 ggcggtgccc tcgatgatgg gcttcaccag ggtgggcagc tcctcgggct ggtgctggcc
      601 gtcggcgtcg atcgatacca cgatgtcggc tcccagcttc agcgcgaggt cgtaccccac
      661 gcggagggcc agaccgccgc cccgtcggat cggcaggcgg gcggtgagcg ccccggccgt
      721 acgagccgcc ccggcggtgc cgtcgtccga gcagtcgtcg atcacgatgg ggatcacccg
      781 gtacccctcg atctcatcgg gcatggcgtg gatcaccgcc ggcacgttct cggcctcgtt
      841 gaaggcgggg gacaggacca cgatccgggg accgggaggc agcgcgtggg cctgctccca
      901 gtcgaacgtg ctctgcccca gggactccac cagcagccgg atgctccgct cgctcgagtc
      961 caccgccccc tgcatccgga gcagcagacc ggccatgatg atcaccgcga tcaacagcac
     1021 ggccgtgagc cggcgcccgc tccccggccg gaagttcaag atgtcgaaca cggggttgaa
     1081 caggttcggg gcgatggcca gcaggaccac ccccaccgag atcagcgccg tgacgatcag
     1141 cgtcagtcgg ctgacctgcc gacgctggtg accgaccacg gcgccgccga acagcaccac
     1201 cgccgccagg gctccgatga tccgtaggac gatcatgctc ggcggctcct ccccgctccg
     1261 gtgcgcccta ggtggcggtc ggttcgtgca gcggggcccg ccaccgcgtg cgcggcatga
     1321 tgtggtccag gatcacccgg tccttcagcc gctcgatcgc ggcgatctcc gactcgatca
     1381 gggtctccga gagcaggtgc ggctccaggc ccaggtccag cagcttcgtg tgcttggcgt
     1441 tgtagtagtg ctcctcggcc tccacccggg ggttctcgac gtgatcgatc ccgacccgga
     1501 gcccgtggtg ctggccggcc tccaccacct tgtgcgcgag ctccatgatg gagaactgct
     1561 cggtgaactg gttgaacacc cggtactcgc cctcggcggg cggattctcc accgccagct
     1621 ccacgcaccg gagggtgtcc acgatgttga ggtacccgcg cgtctgtccg cccttgccgt
     1681 aggcggtcag ggggtgcccg atcaccgcct gcaggcagaa acggttcagc gcggtcccga
     1741 acacctcgtc gtagtcgaac cgggtcacca ggcgggggtc gagcttggtt tcctcggtct
     1801 cgatgccgta cacgacgccc tggttcaggt cggtcgagcg caggccccag acgcgacagg
     1861 cgaaatggat gttgtgggaa tcatggacct tcgtgaggtg gtagaagctg ccgggcatct
     1921 tcgggtaggg gagcgtgtcc gatcgcccgt tgtggtcgat cgtgatgaag ccttcctcga
     1981 tgtcgatgtt cggcgtgccg tactcgccca tggtcccgag cttcaccagg tgggcgttcg
     2041 gggcgtactc ggccatcgcg tagagcacgt tcaaggtgcc catcacgttg ttggtctggg
     2101 tgaagaccgt gtgctcgcgg tcgatcatcg agtacggtgc cgagggcatc tcgccgtagt
     2161 ggacgatggc ctccggctcg aactcccgga agacacgctc gacggtctcc cagtccagca
     2221 ggtccccctc ggtgacgccg atctccagcc cggtcgcgtc cttccaggcg gcggcacgag
     2281 ccggcaggtc ctcgaggatc ggggtgagtg agtcggtgcc ggcctcatcg tgcgctcgac
     2341 gccggaggta gttgtccacg gcatggacct ggtggccgcg ccgagagaag tacatcgacg
     2401 tcgaccaccc caggtatccg tcgccgccca ggatcaggat gcgcaatgga gtccctttcg
     2461 tctcaccgtg ggacggggag gtatcgtacc cgacatcgcc gcggttcccc tgtcgggacc
     2521 agcgccgcgc gaggaggacg ggatccagcc cgccgcggcc gtgaccagca ccaggacgat
     2581 gccgggcgcc ccgccgagat ccaggccgag cgagccaccc accacgccga ccagcacgag
     2641 cgcggcgagt ccgagcccgg gtgagagtgc gacccgcgcg atgtcgaacg agcgcccgcg
     2701 caggacgcgg gaccatccga tgccgacgcc gaagagaagg acgaggacga acgcggcgtc
     2761 catgatcctg gtcgcatcgc tcgggctcgg gaacggtgcc ggtcggatcg gaccggtcgg
     2821 tggcggtccc tcgaccaggg tgacgccggg ggcgatcggc acgccgggac cggtatcgga
     2881 caccggtcga ccgaacgagg ccagggccag gatgatcggg tgctgttcga ggagcggacg
     2941 gacctcccgg aaggtcgctt cggaggctgc ggtgatggag ttgccctcgc ggatcgtggg
     3001 gtgaccctcg agcaggtccg ccgagcgccc cacgaacaga tgcggtctgg cgatctgatc
     3061 cgtgggcagc aggctccgca gcaccggatc ctgcttcacg cggctcggtc cgctcaccac
     3121 gacgatcgcg gggcgatccg ggaaccgctc gaggtactga ccgaccgcgg cggcttccga
     3181 ggcctgccgg atggtgaacg ctggcgctcg ctgctcccag gcatgccatc cgggaaggat
     3241 cagaccgagg atcccgaggg cacccagccc gaagatcgag gcggtcacca ggcgccccca
     3301 aggaacggct gtccatctgc gggaagcgcg tgccaggcag cggcctcccg cgacgagtcc
     3361 cgccagcacc aggacgggga cgccgagcgc cgagcccacg acgcggtacg cggggaccga
     3421 gaggtgcagc gtccggtacg cgagcatcgc ggcgggcacg gtgatcgccc agagggccat
     3481 cagggcgacc gaggtccgcc gcaggccctc ccgcgtcgtc cacaaccaga agacgccgat
     3541 accgatcgcc agcagggcca ggatcaggtc gcttgggcca agaagccgct cgaacttgcc
     3601 ttccagcgtg gcggccgtga tgtcggggat ccggatgtga gccagtccga gcaccagcag
     3661 cagtcctact gcggatagcc cggcaccggc caacacgagc cccaggcgtc cggacggcgt
     3721 tcgccgcgcc ggggcgcctg ctcgatggag ccggatcgat gaggggatct ccaccagggc
     3781 cagggcagcc accagggctg agaactctcc cgcgaagatc cagtgggtcg ccagggcggc
     3841 ggccaggagc gtcaccggga cgatgaggct cagtcggtcc ttcgtcgcca gcaccacagc
     3901 cagcgctgcc accaccagga gggcgtccac gagcaggttg tcgccgtggc cgatcgcggt
     3961 gcgagcgagg tccacggaga acgcgacacc gagcgcgtac agcggtgccg tccacggagg
     4021 gtccccgagc cctcggagcg cgaaccctgc cgctgccagc ccgatggtca cggccatcac
     4081 ggccgggagg acgaaggcga gccgaagggg cgtgatcccc gatgtcgcat gcaccaacgc
     4141 cgcgaagatc ggataggcgg cacgctcggc gagcgggtcc tggttgggca cgtggatctc
     4201 ggcgagcgcc cggaagccgt gatccgtgac gatgttcgag cgccagatgt accggggcgt
     4261 atcgaacccg gagggcaccc gcggagcatg ggctccgaac ggggcgaggt agaacgcgat
     4321 gatcgctgcg gcggccgcgc cgagggcgat cccaacgacc acccggccgg agggccggcg
     4381 ccgggcatgc gagcccggct cggatccgcc cctttgcggg ctcacttcgg tgtcggccgc
     4441 ccccttgccc cgggtcttcc agcatcggtt gggtctgggc gccaaggccc ggatgaagcc
     4501 atccgtcctc cgtggtccgc gtcgcccatg ccgctgcgcc ggtcgtccgc ccccctgcac
     4561 gccggggatc cctcgcagag ccccatcgtg gtgacgatgt cccggatggc gatgccggtc
     4621 aagcgagcag gctcagggcc tggagcaccc ggtcgacctc ctcggttgtg ttgtagtggc
     4681 agaagccgat ccgcaacgcc ccgccatcct cctccaggcc gagccgctcc atgagctcga
     4741 gcgcgtagta gttcccgtcc caggtgaaga tcccctgctc cccgagggcc ttcgccgtat
     4801 cggccgggtg ctgcccctcc agcctgaccg cgaaggtggg ggtccgctcc gcgagcctgg
     4861 ccggatcggc gatgccgtac agccgcagcc gcggcacgga ccgcacgccg tccaggaacc
     4921 ggcgcgccag ttccgactcg tgcgagccga tcgcttggaa ggccgcctcc agagcggggc
     4981 ggcgcccctc cctcgcgggg gagccgtacg tgcggccgat ccccgccagg tagtccaccg
     5041 cggcgccgaa cccggccaac ccttcgtggt tctgcgtgcc ggtctcccat cggaagggga
     5101 ggtgttcctc ggccgggcgc accttgtacg gccggagctc ggacagcagg tcccgcctcc
     5161 cgaacaggat cccgaggtgc ggtccgaaga tcttgtacgg ggaacaggcc agcagatcgc
     5221 acccgagctc cccgacgtcg atcaggcggt gctgcgcgta gtggacggcg tcgcacacca
     5281 ccagggcgtc gctacgctcc cggacgcgcc ggacgagctc ggcggcgggg gtcaccgtcc
     5341 ccaccgcgtt cgaggcgagg gtgaaggcca cgaccttcgt ccgtgctccg acggcggcat
     5401 cgaacgatcc gaggtcgagg gtcacgtgct cctcgtccag gtccacccag cgcaccgtgg
     5461 ccccggcgtc ctccgccgcc atgacccagg gccggacgtt cgcgtcgtga tccagccgtg
     5521 tgaccaccac ctcgtccccg ggccccaggg tgcggccgat ggatcgagac acggcgaaca
     5581 ggagcgtggt ggcgttcggt ccgaacacgg cctggtccgg ctcggagccc accaggtcgg
     5641 cggccgcccg gtgcgcctga tcgacgatcg catccgtctc ctcgctcgtc acgaacgcgc
     5701 catgagagtt ggcgttgctg gtccgcaggt aggaggccat ggcttcgatc accacgtcgg
     5761 gcacctggga gcctccgggg gcatcgagga acgcgcaggg ccgtccatcg atctggcggg
     5821 tcagggccgg gaagcgagcc cggagcggcg acaggtcggg cacggtcacg gcaagagctt
     5881 gcgtgacggg accgccgccg acaacccgct tctggctgag cgctcggtca gtccacccgg
     5941 tcccagtccg ccggcagctc cggtgcggcc cactccggtg gcggacgcca gtcccgccaa
     6001 tcatggtcga aggggaccag gcggtccttc agcagacgga cggcctcctc tccgtcccgg
     6061 cggaacccct gcgcctgccg gggcgagaac agcccgcggc ggagcgcctg gtccagctcg
     6121 tcctcgtcct tccagctcca ggaggagcgg tccggcggga gcagcacgtc caagacgtgg
     6181 tcggtgtagt cgaaccccac cggggtgcgc cgcagcggcg actccaggtt cacgtaccag
     6241 tgtaggggct cccaatcccg gtcccagaac agcatgggcg cgtaggaccg gccggggaga
     6301 gcgaacgaca ggatggctcg gggaggatcc caggtccgat cggccagcac ccatcggggt
     6361 tcgggcagct tcagccaccg tccgtccggc gcgcgagggt acttccacac cgtccccgga
     6421 tggacgaaga gcaggcgaag gtcggaccgg tcctccacga cccgcgccgg gatcgcagac
     6481 cagacgcggc cgtcccagat ctcgcgaagg gccaccgccc tccccggctc gaaccggtca
     6541 tcgggtggtc ccgcgctcat cccatccgct cggcgaaggc ggcctcgacc tggtccagga
     6601 gggtgcgggc acgcgctcgg agggagtcca cctcctcgcg cagctcggcg ctcttggcct
     6661 ggtccttcag cgaggcgacg ttgatctcca cgttgtagat gccgcagcgg gcggcggcgt
     6721 agagagcctc ggcggcggag gctccgtcgg aggcggcgtt ctgattgccg atcctgaccg
     6781 cgttggccgc catgtcgatc aggtccacgg cccggcgggc gatctcgagg ggcaccaggg
     6841 cggcgcccag gaaggcgctc tggatggcct gccggcgctc gtcgatctgc tcttcggtct
     6901 ccttggggag cctgaaggcc gtcatgacgg cctcgaaggc tgcggcgtcg cgatcggcca
     6961 ggtccaggaa ggcccgccgg tcctcctcgg cccgaccgag ggcctcccgc atcggctccc
     7021 aggcgctctc gtacccctcc ttgtccaccg tgagccgagc caccatggag atcagcgaag
     7081 cgccggtggc tccgaagagc ccggcggccg ccccgccgcc cggggtggga cgatccgagc
     7141 tgaggacgtc caagaagtcc ccgatcctcc gcgagccgat ccccgacgag gcctcggccg
     7201 cgctcaccag acgctccagg atctgtgcat ccgcatcgaa cccctccagt cgtagctcct
     7261 cgatgtccgc atccgagatg gcggcctggg gcaccaagcc cacgatctcg ctcgagcgca
     7321 cctccaggcc ccggcggcgc gcctcgtcgc ggacggcccg gaacgccgcg cgcaggcccg
     7381 tgacctcgtg gtcgacgagg ttcatcgaca ccgtgacgca gtcgcgctgg ggcacgtcga
     7441 agccgatggc gcggaccgcc gggagcccac cggaggactc gcggaccgcc cgcgcgacgt
     7501 ccttggcggc cgcctggtcc ccgccggaca ggtagacgtt gaaggcgatc agggccttgc
     7561 gggcccccac cgcggtggcg cccgcggggc cgatccgggc cggaccgaag tcgggcagcc
     7621 gctcgccccg ctccaccgcg gcccggagct cctcgtactc gccgcggcgg acctcggcca
     7681 ggctgcgccg gccctcggac agggcggccc ggtcgtagca gtagacgggc aggtccagcc
     7741 gctcggccag ctcttgcgcg aaggcccggg cgaaggccac gcagtcgtcc atggtgatgt
     7801 cgcgcacggg catgaacggg atcacgtcca cggcccccat gcgcgggtgg ttgccccggt
     7861 gccgatccat gtcgatcagc cggacggcca cggcggcggc gccaagagcg ctccggctga
     7921 cggcccgggg ggaaccgatg accgtgcaat cgagccggtt gtggtccgga tcggcctgcc
     7981 gatacaccag gtgcgcgcca ggctgctcca gcgctcggca gatctcgtcg atcacgtcct
     8041 ggcggcgacc ttcgctgaag tttggaacgg cggcgatcag caccccgttg tcccgcacgc
     8101 tcacctccag ttccgttccc accgggatcg gcgaccgtcg gccgcccccc acggacccat
     8161 ccacgacccc cggaacgata cccgggcccc ggcgctctcg aagcgtgccg tttgacggat
     8221 gtccgcgggg ggaggtacat tccccttctc tcgaacatac gttcgatagg gataggggat
     8281 ggcagcgagc gagcggaccg aggcggcgag cgatgtcgaa gcgctacgac gaggtggtcg
     8341 aggtcctggt ggaggccgac gcggggatgc cctccgcgtt ccggtggcgg ggccggcggt
     8401 acgaggtggc ggacgtgatc ggccggtggc ggatcgaggg ccggtggtgg gcggacgggc
     8461 gcgatcgcga gtactggcgg gtggaggcgc ggggtggcgc ggtgtgggac ctgtaccacg
     8521 accgcctcgg cggccgctgg cacctcgagc gtctgtggga ctgaggggga agtagcatcc
     8581 gggtgtggat cccgtcgagt cggctcgagc cttcgcccgg cagatccgct cgtacatcga
     8641 cgactgggag ctcgaggcca cctatccccg ccccgtggtg gcgtccaccg gcctgaccgg
     8701 cctgtatgct ccgggtgagc tcggcgggct cgggctctcc tacaccgagg cggtcgaggt
     8761 cttcgaggag ctcggccggg ccgacgccgc catcgccttc tccctgtcca tgcacaacgc
     8821 ggtcaccgcc atcgtcgccg atgccggcga cgatggcctc cgtcaccggt gggcgaaacg
     8881 gctgatccgg ggcgaggcgc tcggcggctt cagcctcacc gagccgcacg ccggatcgga
     8941 cgcgaccgcc atcaccacca gggcggtcca gacacaggtc ggctggcgga tcaccggctc
     9001 gaaggcttgg gtgtccctgg ccggtgaggc ggacctgttc ctggtggtct gcaagacgtc
     9061 ggaccagccc ggccatcggg acatcgcggt cctggcggtc cagcgggagg gcgggggtgt
     9121 gagcttccct cggatctacc gcaagccccg ggccgcgttc ctgccgatcg gggagatgga
     9181 gctgaacgat gcgccggcca cgctggtggc tccccccagg accgggatgc aagccgcgct
     9241 gggggccatc gacgtggccc gctgcatgat cggcgccatc gcgaccggga tgcacgcgga
     9301 ggccctcgat ctcgcgctgc ggtacgcacg ggaccgcaag ctgttcggcg ggcgggtgat
     9361 cgaccagcag gggctgcagt ggagcctggc cgacgcggag acggacctgg tggccgggcg
     9421 tctgctcacg cggcgcgccg ccgaggcgct cggctccgcg gagggcacgg tggccgtggc
     9481 ccacgcgaag cgcttctgcc ccgacgccgc gctgcgggcc gtggtgctgg ccagccagat
     9541 cctcggtgcc tacggctggc tcgagaacca tccgctggtg cggttcatgg acctggcgaa
     9601 gatgctccag acggtcgacg ggaccaccga ggtccagcgc gtgatcatcg cccgcgacct
     9661 cctcaagcgt gccgaggagc tgggcccgga gccgtcttga cgccatccgg cgtgaggaag
     9721 tagcgtcgcg ggtatcgaac gtatgttcgg gtcgatatgg cgacgacctc ccccggcttc
     9781 gtgcacctcg acgcccggtc cttcttctcc ctgaaggacg gggcgttctc gcccgaggac
     9841 ctggccgggc gtgccgccga gctcggcatg ccggcggtcg ccctcaccga ccgcgacggc
     9901 ctctacggcg cggcccgctt cgtcgccgcc tgtgcgcgcg agggcgtgca gccgatcctc
     9961 ggcgccaccc tcaccgtccg cgatgtgctc ggtggtggcc gaggaggacc ctccccctcc
    10021 accaccgtga tcctgctcgc gcaggaccag cgcggctacg cgaacctgtg ccggctcctc
    10081 accgatgcgc acatggcggg ggagcggggg gagccgtcgc tcgcgccgga gcagatctgc
    10141 gcgcaccccg aggggctgct cgcgctgctg gggccggact cgctccccgg cgggctggcc
    10201 gtcgccggtc ggttcgacgc ggcccggcgc gccgcggagc cgtaccgcga ggcgttcgga
    10261 ggtcggctgt gggtggcggt gcagcaccgg ctggaggcca actcggcggc ggaggtccgg
    10321 atgctgctgc ggttcgccga gtggatcgcc gcgcggacgg tggccaccaa cccggtccgg
    10381 tacctggtcc cccaggacgc cttcctggcc gacgccctgg agtgcatgcg gcagatcgtc
    10441 ccggtgaacc accgcaacgt ttcccggacc aacgccgagg ggtacctgaa ggcccccgcc
    10501 gagatgcgcg agctgttccg cgagcgcccg gacctggccg accggaccct cgagctgacc
    10561 gagtcgtgca ccttcgacct cggcctgggg caggtgcact tcccggcgtt ccccacaccg
    10621 gcgggccgct ccgccgcctc ggtcctggcc gagcgctgct ggcgcgggat ggagcggagg
    10681 ggcttggaga ccacccggcg ggtccgggac cgcctggacc acgagctcgg catgatccgc
    10741 cggatggggt tcgccgccta cttcctgaac gtggccgaca tcgtggcgga catcaaggcc
    10801 atggggatcc gctgcgcgtg ccgggggagc gcggccggct cgctggtctg ccacctcacc
    10861 gggatctccg acgtcgaccc ggtccggcac gacctggcgt tcgagcggtt catgaacccg
    10921 ctccgggacg agctcccgga catcgacgtg gacgtcgagt cggcccggcg cgaggacgtc
    10981 tacgacatga tcctgtcccg ctacggccag gaccggtgcg cctgcgtggc gatggtcgac
    11041 acctaccgag cccgctcggc gatccgcgag gtgggcaagg cccttggctt ccccgaggtc
    11101 gaggtggaca cggtggccaa ggccttcccc cacatcggcg cgggcgacat ccgcaaggcg
    11161 atcgagatcc tccccgagct ggccggctcg aacatgaacg ccggccagct cgggctgctg
    11221 ttccgggtgg ccgagcgcct gaacgggttc ccccggcacc tggcgctgca cccctccggc
    11281 ctcgtgatct cgaacgacga cctggtccgg cgggtgccgt tggagcgctc gttccagggg
    11341 taccggatga tccaggccga caaggacgac gtcgagctgc tgggctacct gaagctcgac
    11401 gtgctcgggg tgcggatgct ctcgtcgatg cgccacgccc tggacgagat cgcgcgcacc
    11461 gaggcggtca aggtcgacct ggacaggatc ccgctggacg acgagccgac gttcgagctg
    11521 atccgggcct cggacacctt ggggtgcttc cagatcgagt cccccggcca gcgcgagctg
    11581 ctccagaagc tccagcccac ccgctgggag gacctgatcg tcgacatctc gctgttccgg
    11641 ccggggccgg tgaagtcgga catgatcggt cccttcctgc ggcggcgggc cgggatggag
    11701 cgcccggtct acctgcatcc gtcgttccgc ccggcgctgc aggagacctt cggcgtgatc
    11761 gtctaccacg agcaggtgat ccgcaccgtc gccgccgtca ccggctgcga cccctccgag
    11821 gccgaccggg tccgccgtca cctctccgac gatctcgagg tgggccggat ccgcggctgg
    11881 ttcctggagc gcgccaagtc caacctggcc ctgtccgacc gggacgccga gcggatctgg
    11941 agggaggtct ggtccttcgc cagcttcggc ttctgcaagg ctcacgccgc cgccttcgcc
    12001 gtacccacct atcagtcggc ctggctgaag acccactacc cggcgcactt cctggccggg
    12061 gtcctcaccc acgaccccgg gatgtacccc cggcggctga tcctcgagga cgcccgcgag
    12121 cacggcatcg ccatcctgcc cctggacgtg aaccgcagcg agcgggacta cgtggtcgag
    12181 ccgtcgctct ccgcggctcg cgccgtcggg tccggcacca ccgcgaccac tgccccgatc
    12241 aacacgtacg ggatcaggtt ggggctgaag gacgtgcatg ggatctccga tgccgagatg
    12301 cggtcgatcc tggaggcgcg cgccgaccgg ccgttccggg acgtggggga cttcctgcgg
    12361 cggacgaggg tggcgcggcc ggtggtggag gcgatcgcgc acgccggcgg gttcgacgct
    12421 ctgcccggcg gcacccgacg cgaccgcctc tacgaggcca tggtcaccga gccccaacgc
    12481 gaaggcgacc aactgaccct cgacctctgg tcggcaacgg ggtcggtcac ggggttggcc
    12541 accgtggcag tgcagcggga tgccggagct ccgggcaacc agaggggctc tcagccccac
    12601 tccctccccg agtacaccga cgccgagcgc gtgcgagccg agctcgaggt gctcggcttg
    12661 gatgcctccc gtcacctgat ctcgttcttc gagcccctgc tcgccgacct cggcgtggtg
    12721 cgctcctgcg atctgtggcg tcaccgctcg gaggcctggg tgatggtggc cggggtgaag
    12781 gtggcctccc agacgccggc cgtccgcagc ggccagcgga tcgtcttcct cacgttggac
    12841 gatggcacgg gcccggtcga tgtcaccgtg ttcgagaagg tccagcccaa ggtggtgaag
    12901 acggtgttcc acgcgtccgt cctggcggtg tgggggcgcc tgcgccggac cggggtgaag
    12961 ggcgtcagta tcatcgcgga agatgtctgg gacctggtcg cgctgcaagg ggcccgccgg
    13021 gagggacgcc tcgagcggtc gctcgcggag cacgcttcct cgcccgcgcc gtctccacgg
    13081 aagttctggc acgccagcgg gggttcggcc ggatggtgat ggaacggatg ccctcggatc
    13141 gccgccgcgg gatcccggcc ggcccggagg ggtgagggtg gatggggcct ccccgagccg
    13201 ctgaggcgat cctccacgtc gatctggatg ccttctacgc ctcggtcgag gtgcagaagg
    13261 acccctcgct cgccggcaag cccgtggtgg tgggtggggc gggaacgcgc ggggtggtga
    13321 tgtcggcctc gtacgaggcc cgggccttcg gcgtgcggag cgcgatgccg gcggtgcggg
    13381 ccaggcggct gtgtccggac gccgtcttcc tcccgccgga cttcgagtcc taccgggcgc
    13441 actccaaccg gttccgggag atcctgctct cgttcactcc gctggtggag cccatcgccc
    13501 tggacgaggc cttcctcgac gtcgccggcg cggagctgct gttcggcccg ccccgccgga
    13561 tcgccgagct gatccgggga cgggtccgca ctgaggtggg ggtgagctgc tcggtgggtg
    13621 tggcgcccaa caagctgctc gcgaagctgg cctcgcggcg ggccaagccc gacggcttgg
    13681 tggtggtccc ctcggacggg gtggcggcct tcctcgagcc cctgccggcg acggagctgt
    13741 ggggcgtggg ggagaagacc atggaggtgc tgtcccgtct gggggctcgc accgtgggcg
    13801 acctcggtcg gatcccgccc ggggtgctgg gccggctgat cggcgaacgg cacgccgccg
    13861 acctgcgcga cctggcccgg ggccgggacg agcgcgccgt gatgccgtac gaggcgccga
    13921 agtcggtctc gcacgaagag accttcgccg tggatctgga tgccgaggag gacctgctgc
    13981 gggaggcgct ggcgctctcg cataaggtcg ggtccaggct ccgggaggac ggctaccggg
    14041 cccggacggt cgtcctgaag gtccgcctgg ccaacttcac gacgctcacc cggtcccgaa
    14101 cgctccccgg agccaccgac atcggggccg agatcttcga cgtggcccgc gacctgtacc
    14161 gagggttgcc gggcgcacac cgccgggtcc ggttgctggg ggtccaggcc acggggctgg
    14221 tgccggccgg ggccgaacag ctcgcgctgc tgcgaggcga gcggtggggc gacgtcgagc
    14281 gggccgtcga ccgcatcgag cgtcggttcg gacggggggc cgcccggccg gcatccctca
    14341 tggatcgcag tcgggggcgg tcaccaggtg agcaggtggg caggtgaagc gggtgggctg
    14401 ggtgggtaaa
//
LOCUS       DASQQQ010000082         3393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_144138, whole genome shotgun sequence.
ACCESSION   DASQQQ010000082 DASQQQ010000000
VERSION     DASQQQ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144138"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VE646_03435"
     CDS             complement(<1..491)
                     /locus_tag="VE646_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HZD79080.1"
                     /translation="MSDPFRARLQRAAHQAGEAGLAGLLVTPGPDLRYLSGHAPPPLE
                     RLTLLALRPGRDPVMLLPALEAPAARSAPGIGTVELTTWEDGEDPYAAAARIVGVGRY
                     AISDQAWATHVLGLLGAISGCELVPRGTALPLLRARKDREELRLLARAGAGADASFRV
                     VRT"
     gene            complement(488..1414)
                     /locus_tag="VE646_03440"
     CDS             complement(488..1414)
                     /locus_tag="VE646_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HZD79081.1"
                     /translation="MATPFGKLTVIVNPHAGKRRVGEEIPELERTLQARGLPYTLLRT
                     EGPGDATRFAREALESGARFVVAVGGDGTVHEVVNGMIRDDRPIVPQPVLGVVAAGSG
                     CDLVRTFGLPGDTTRSCQHLMGENTYPLDIGKVTFTGPDGGPAVRYFPNVAEVGLGAA
                     VTARAGRMPARFGQSKYFFAFWRTLPGYRLAGLKLQADRRTYEGPGLMVIVGNCQYYG
                     GAMRISPRSYPGDGVLDVLVFKGPKSDAFTLLPKVYRGEHLPHPHIAEFRVKRSLSVD
                     ADRPLALEADGEVLGTTPATFEILPQPVLMKL"
     gene            complement(1476..>3393)
                     /locus_tag="VE646_03445"
     CDS             complement(1476..>3393)
                     /locus_tag="VE646_03445"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HZD79082.1"
                     /translation="RPREGHRRVYVPRREEVVQVLGEQGMLPAIYFVFSRAGCDRSVE
                     WLLGSGLRLTEREEAERIREFAEMRVAWMDEEDLSTLGFYDFAEALSAGVAAHHAGML
                     PVFKETVEELFEAGLVKVVFATETLSLGINMPAKTVVIEDLWKFQGERHELLTPGEYT
                     QLTGRAGRRGIDELGHAVVVFQRQVPFERVASLATTRTYELTSSFRPSYNMAVNLIRN
                     YTRDQAHHLLNSSFAQFLADRGVVVLERQLERDRAYLDGYHQQMACRLGDFREYWRLR
                     QKADRIRDEARRGRDRVRGDAVREALGALRPGDVIFVPRARRRGLAVVLSSREGRPTV
                     LAQDRKYFRLSPRDFEDPPVVLSKVTLPRSGSVRSARYRRDLAARLVALEVRPPKGRR
                     DRVDRQAEREAARYERAAEQHPCHRCPDRPTHERWAARASQLERQVQGVERRIRNRTE
                     TLARQFERVLGVLEELGYVRGFQTLPKGELLARIYGEGDILVTEALADGAFEGLSPQE
                     AAAVISSFVYESRERVPRHAEMPTAETVDRYRRILGLWRRIRRSEDQHHVELCRELDP
                     GFAAPVFHWAEGKPLEDVLAETEMAPGDFVRNCKQLLDLLRQIEEVAEGPASAPVGRA
                     TEAVNRGVVAYTGV"
BASE COUNT          475 a         1283 c         1176 g          459 t
ORIGIN      
        1 tgggtccgaa cgacccggaa ggacgcgtcg gcgccggccc cggcgcgcgc cagcaggcgc
       61 agctcctccc ggtccttgcg ggctcggagc agcggcaggg ccgtcccccg gggaacgagc
      121 tcgcaccccg agatggcccc gaggagcccg agcacgtgcg tcgcccaggc ctgatcgctg
      181 atcgcgtagc ggccgacccc cacgatgcgg gcggcggcgg cgtagggatc ctcgccgtct
      241 tcccaggtgg tgagctccac ggtgccgatc ccgggcgccg accgcgccgc cggcgcctcc
      301 agggccggca ggagcatcac cggatcccgc cccggccgca gcgcgagcag ggtcagccgc
      361 tccagcggag gcggcgcgtg gccgctcagg taccgcaggt cgggcccggg cgtcaccagg
      421 aggcccgcga ggcctgcctc gccggcctgg tgggcggctc gctgcaaccg ggcgcggaac
      481 gggtcgctca cagcttcatc agcaccggtt ggggcaggat ctcgaaggtg gccggcgtgg
      541 tgccgagcac ctccccgtcg gcctcgaggg ccagggggcg atcggcatcc accgacagcg
      601 accgcttcac gcggaactcg gcgatgtggg ggtgcggcag gtgctcgccc cggtagacct
      661 tgggcagcag ggtgaacgca tccgacttcg gccccttgaa gaccagcacg tcgagcacgc
      721 cgtctcccgg gtaggaacgg ggagagatcc gcatcgcgcc cccgtagtac tggcagttgc
      781 ccacgatcac catcagcccc gggccctcgt acgtgcgtcg atcggcctgg agcttcagcc
      841 cggccagccg gtacccgggc agggtgcgcc agaaggcgaa gaagtacttc gactgcccga
      901 agcgggccgg catccgcccg gcccgagccg tcacggccgc ccccaggccg acctcggcga
      961 cgttggggaa gtagcgtacg gccgggccgc cgtccggacc ggtgaaggtg accttgccga
     1021 tgtccagcgg gtaggtgttc tcgcccatca ggtgctggca ggagcgggtg gtgtctccgg
     1081 gcagcccgaa cgtccgcacc aggtcgcagc ccgagccggc cgccaccacc cccaggaccg
     1141 gctgggggac gatcggtcgg tcgtccctga tcatgccgtt caccacttcg tggacggtcc
     1201 cgtccccgcc gaccgccacc acgaaccttg ccccgctctc caaggcctcg cgcgcgaacc
     1261 gcgtggcatc cccggggccc tcggtccgca gcagcgtgta cgggagccct cgcgcctgca
     1321 gcgtgcgctc cagctcgggg atctcctccc ccacccgccg cttgcccgca tgggggttca
     1381 cgatcaccgt gagcttgccg aacggggtgg ccatggcggg tgcatcctat cgacgtgggg
     1441 ccccggtccg gcggagcatc cgacctggtg gcacctcaga cccccgtgta ggccaccacc
     1501 ccgcggttga cggcctccgt ggcccgcccc accggggccg aggccgggcc ctcggcgacc
     1561 tcctcgatct ggcgcaggag gtccagcagc tgcttgcagt tgcgcacgaa gtcgcccgga
     1621 gccatctcgg tctcggccag cacgtcctcc agcggcttgc cctcggccca gtggaacacc
     1681 ggagccgcga accctgggtc cagctcgcgg cagagctcga cgtggtgctg gtcctccgac
     1741 cggcggatcc gtctccacag accgaggatc cggcggtacc gatcgaccgt ctcggcggtg
     1801 ggcatctcgg cgtgacgtgg gacgcgctcg cgcgattcgt agacgaacga ggagatcacc
     1861 gccgcggcct cctgggggga gagcccttcg aacgccccgt ccgccagggc ctcggtcacc
     1921 aagatgtcgc cctccccgta gatccgcgcc aggagctcgc ccttcggcag ggtctggaag
     1981 ccccgcacgt agcccagctc ctcgagcacc ccgagcaccc gttcgaactg cctggccagc
     2041 gtctcggtcc ggttgcggat ccgccgttcc accccttgga cctgccgctc cagctgggag
     2101 gcccgggccg cccatcgctc gtgggtgggc cggtccgggc agcggtggca gggatgctgc
     2161 tcggcagccc gttcgtagcg ggcagcctct cgctcggcct gccggtccac gcggtcccgc
     2221 cggcccttgg ggggccggac ctccagggcc accagccgcg ccgccaggtc ccgccggtac
     2281 cgggcgctcc gcacgctgcc cgagcgcggc agggtgacct tggacaggac caccggagga
     2341 tcctcgaagt cgcgcggaga cagccggaag tacttccggt cctgcgccag gacggtgggc
     2401 cggccctcgc gggaggagag gaccacggcc agaccgcggc gccgggcccg tggcacgaag
     2461 atcacgtcgc ctggccgcag ggcgcccagg gcctcgcgca cggcgtcgcc ccgcacccgg
     2521 tcgcggcctc gccgggcctc gtcccggatc cggtccgcct tctgcctgag ccgccagtac
     2581 tcgcggaagt ctcccagccg gcacgccatc tgctggtggt acccatccag gtaggcccgg
     2641 tcccgctcca gctgacgttc caggaccacc accccgcggt cggccaggaa ctgcgcgaac
     2701 gacgagttca gcaggtggtg ggcctggtcg cgcgtgtagt tgcggatcag gttgaccgcc
     2761 atgttgtagg agggacggaa ggaggaggtg agctcgtacg ttcgggtggt ggccagcgag
     2821 gccacccgct cgaaggggac ctgtcgctgg aacaccacca cggcgtgccc cagctcgtcg
     2881 atcccccgtc ggccggctcg gccggtgagc tgggtgtact cgcccggcgt caggagctcg
     2941 tgccgctcgc cctggaactt ccacagatcc tcgatcacca cggtcttcgc cggcatgttg
     3001 atgcccaggg acagcgtctc ggtggcgaac accaccttga ccagccccgc ctcgaacagc
     3061 tcctccaccg tctccttgaa gacggggagc atcccggcgt ggtgggcggc caccccagcc
     3121 gagagcgcct ccgcgaagtc gtagaacccc agggtggaca ggtcctcctc gtccatccac
     3181 gccacccgca tctcggcgaa ctcacggatc cgctcggcct cctcccgctc ggtgaggcgg
     3241 aggccggagc ccaacagcca ctcgacgctc cggtcgcacc cggcccggct gaacacgaag
     3301 tagatcgccg gcagcatgcc ctgctcaccg aggacctgga ccacctcctc gcgacggggg
     3361 acgtacacgc ggcggtgacc ctcccggggg cgc
//
LOCUS       DASQQQ010000083         7079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_33160, whole genome shotgun sequence.
ACCESSION   DASQQQ010000083 DASQQQ010000000
VERSION     DASQQQ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33160"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /gene="ccsA"
                     /locus_tag="VE646_03450"
     CDS             <1..348
                     /gene="ccsA"
                     /locus_tag="VE646_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HZD79083.1"
                     /translation="VGSMTKRGLLPPAAVLDRIAYRTIAFAFPIWTFAIIAGAIWAEN
                     AWGRYWGWDPKETWSFITWVVFAGYLHARATSGWRGRRAAVIAMVGFASLFVTYYVVN
                     LWIVGLHSYAGVS"
     gene            500..1573
                     /locus_tag="VE646_03455"
     CDS             500..1573
                     /locus_tag="VE646_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HZD79084.1"
                     /translation="MRKLSRLVLGAVALALVAAACAPNASQDSLKPAGPYAEKIHALF
                     TPVFWIAAAIFFLVEGMLIFFVIRYRHRKGRERIPPQIHGNNRLEIAWTIVPALILVG
                     VTVPTVATIYDLASKPTGRVLNVNVIGHQWWWEFDYPGQKIITANEMHIPVGEPVYVS
                     LCSAGAGYKQQPAPNTCQDVPAGVGDAVIHSFWVPELAGKQDVVPGRTNTLTLEADEP
                     GTYSGQCAEFCGLSHAYMRFKVIAEPRDRFDQWVRAQQTDADTPPEGSLAATGMNQFL
                     NGQCIACHAIQGTDAQANGGPNLTHLAGRDCFAGCSFDLNEQNLKRWLADPPAMKAGS
                     WMPDYGLSQDQIDALVAYLMTLK"
     gene            1590..3464
                     /gene="ctaD"
                     /locus_tag="VE646_03460"
     CDS             1590..3464
                     /gene="ctaD"
                     /locus_tag="VE646_03460"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HZD79085.1"
                     /translation="MASVSLPGEGGWLHRPSASTGFWSWITTVDHKRIGILYGFTAFT
                     FFLIGGSEALLIRIQLARPDQQILDPNAYNQIFTMHGLTMVFLVVMPLSAAFFNYLIP
                     LMIGARDVAFPRLNAFSYWAFLLGGLFIYSGFALGGAPNGGWFGYAPLSTVEPGNNML
                     FYAIGLIITGVASTVSAVNLAVTIINMRAPGMSLFRMPVFVWMALVVQLLLAFSLPII
                     TVGLFEMLFDRRFGTHFFDPAAGGDPVLWQHLFWLFGHPEVYILILPAMGIVSEILPV
                     FSRKPLFGYQFVVFSGIAIGFIGFGVWAHHMFTSGLGPVANTAFGISTMIIAVPTGVK
                     IFNWLGTMWRGDIRFTTPMLFAVAFIGQFVVGGLSGVTHAVVPADYQQTDTYYVVAHF
                     HYVLFAGAIFGLFGGMYYWWPKIFGRRLNERMGKVQFWAMVIGANLTFAPMHILGLQG
                     MPRRIQTYPASLGLGFWNFLVSIGAFIIAASVLLFILNAAASFRRPKDAEADPWDART
                     LEWMTSSPPPAHNFDEIPVVHSLDELWHRKYAESEGGAVVPVPAGGSGEQEVVTGKAH
                     EGGGHAIHMPSPSYYPLVAALGFPLIGYGIIFHWWMAFAGMVLILAGLYGWALEPSAE
                     "
     gene            3473..4081
                     /locus_tag="VE646_03465"
     CDS             3473..4081
                     /locus_tag="VE646_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HZD79086.1"
                     /translation="MALSEHLFESEHEATSTGLPNTKLAMWIFLASDCLLFGALITTY
                     VLYRGASVVGPYPKDVFDIPYTSVSSFVLLASSLTMVLALAAIQRGDLGRMRVWLLAT
                     ALLGMTFVGGQVYEFTSFYREGLGLSTNLFGTTFYVLTGFHGTHVGIGVLMLLSLVWM
                     SFTGKLTTRDPMTVELVGLYWHFVDVVWIVIFTVVYLIPTGK"
     gene            4093..4437
                     /locus_tag="VE646_03470"
     CDS             4093..4437
                     /locus_tag="VE646_03470"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HZD79087.1"
                     /translation="MESTHELQQQGAHPAPISGGHTVRHPSPKEYIRIALILGVVTAG
                     EVTVYYVESVRSMLIPLLFLFAAVKFSLVVLWFMHLRFDSRTYARFFMMGLAGAVTLF
                     IIVLLTFSAFSR"
     gene            4443..5282
                     /locus_tag="VE646_03475"
     CDS             4443..5282
                     /locus_tag="VE646_03475"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HZD79088.1"
                     /translation="MILPHWHPHPDVWLVLGGTWAAYVMAVRRHRGRVPADDRNERRR
                     LWMFSLGMLTLWIASTWPIHDLAEDYLFSVHMIQHLLYMLVAPPLLIAGSPPWLLRRI
                     LGPTPVRVVVRFLVRPVVAFILFNAVLLFIHWPAVVAAQLDSELAHFGIHLLTVSVSL
                     VMWWPVMSPLPEMPALSPPGQMLYLFFQSLAPTIPASFLTFGTSILYPAYAAFPRIWG
                     ISVHSDQLVAGLIMKLGGGLVLWGFVATIFFRWHAREERDGWDALQLRHVEREVRSEL
                     TKP"
     gene            5279..6547
                     /locus_tag="VE646_03480"
     CDS             5279..6547
                     /locus_tag="VE646_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HZD79089.1"
                     /translation="MRSTTRDRLLLPVLMPIGILVVIAAVLFGFSRILLSVSHGAATA
                     TALAVAFGIMVVSGVVAGRAVVKPSHLASMVGAIAGVAMVAGGIALVTIAPATSEGGG
                     SAATLHLVAKDIAFDQTKLSAPAGKGFTIAFDNQDAGLQHNVQIFDNKDFSGDPVFDG
                     ELTTGPEQIDYEVPALDAGTYYFRCVVHPSQMTGELDVVEGGGGAGGGGPTVTVSAKD
                     IQFDTSQIELPADTPATIHFQNDDAGIQHNIAIFPSEKDLQDPVFRGDLVTGPGAVDY
                     QIPPLKPGTYYFHCDVHPTMNGKVVVSGAGGGGGPSATPSGGGGAGGGGGGPTTTSVT
                     AEGIKFGISQIDLPANQAVTLTFENKDAGIQHNIAIFKSESDLSNPLFRGEVITGPAT
                     VQYQIPALPPGTYYFHCDIHPTMNGTVVVG"
     gene            6594..>7079
                     /locus_tag="VE646_03485"
     CDS             6594..>7079
                     /locus_tag="VE646_03485"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZD79090.1"
                     /translation="MRRLLPLVLLLPLLASSACTSSAPDGPTVTSISGPMPTLSGTTL
                     VGPELSPDEYAGRPVVVNFWATWCGPCRREQPVLSAAERSAGGDGPVFLGVNYRDDPA
                     AARAYLKEFHVGYPSLEDASGDLAYRFGVPGLPATIFVDAGGRMRFRVLGALDASTLS
                     DL"
BASE COUNT          994 a         2403 c         2407 g         1275 t
ORIGIN      
        1 gtgggcagca tgaccaagcg tgggctcctg ccgcccgccg ccgtcctgga ccggatcgcg
       61 tatcgcacca tcgcgttcgc gttccccatc tggaccttcg ccatcatcgc cggggccatc
      121 tgggccgaga acgcgtgggg acgctactgg ggctgggatc cgaaggagac gtggtccttc
      181 atcacctggg tggtgttcgc gggctacctg cacgcgcggg ccacatcggg ctggcgaggc
      241 cgccgggcgg cggtcatcgc gatggtgggg ttcgcctcgc tgttcgtcac ctactacgtg
      301 gtgaacctgt ggatcgtggg gctgcactcg tacgccgggg tgtcctgacg gtgagcggcg
      361 gcatcggtcc ggagcggttg gcgttcgtga cggtgtcgtc acgaaccttg cgtcgagcat
      421 gcgcgttcgt ggatggtaca gtgcgccgac cggcccgggt gttcgcaccc cgcgaggtct
      481 ggaagaggta gggggaacgt tgcggaagct gtcacgactg gtcctgggcg ccgtcgccct
      541 ggccctggtc gccgcggcgt gcgcgccgaa cgcgtcccag gactcgctga agccggccgg
      601 tccctatgcg gagaagatcc acgcgctgtt cacccccgtg ttctggatcg cggccgccat
      661 cttcttcctg gtggagggga tgctgatctt cttcgtgatc cggtaccggc accgcaaggg
      721 tcgggagcgc atcccccccc agatccacgg gaacaaccgc ctggagatcg cctggaccat
      781 cgtgccggcg ctgatcctgg tgggcgtgac ggtgcccacg gtggccacga tctacgacct
      841 ggcctccaag cccaccggcc gggtcctaaa cgtgaacgtg atcggccacc agtggtggtg
      901 ggagttcgac taccccggcc agaagatcat cacggccaac gagatgcaca tcccggtggg
      961 cgagccggtg tatgtgtcgc tgtgctccgc cggggccggg tacaagcagc aaccggcccc
     1021 caacacgtgc caggacgtgc cggccggggt gggggacgcg gtgatccact cgttctgggt
     1081 ccccgagctg gccggcaagc aggacgtcgt ccccgggcgg acgaacacgc tcacgctgga
     1141 ggcggacgaa cccggcacgt actccggaca gtgcgccgag ttctgcgggc tctcccacgc
     1201 gtacatgcgg ttcaaggtga tcgcggagcc ccgggaccgg ttcgaccagt gggtccgcgc
     1261 ccagcagacc gatgccgaca cgccgcccga gggctcgttg gccgccacgg ggatgaacca
     1321 gttcctgaac ggccagtgca tcgcctgcca cgcgatccag gggaccgacg cccaggccaa
     1381 cggcggcccg aacctcacgc acctggccgg tcgggactgc ttcgcgggat gctccttcga
     1441 tctgaacgag cagaacctga agcgatggct ggccgatccc ccggccatga aggccggctc
     1501 gtggatgccc gactacgggt tgagccagga ccagatcgac gccttggtcg cctacctgat
     1561 gacgctgaag tgatggaggg agctcgagca tggcgagcgt gagccttccc ggcgaaggag
     1621 gctggctgca ccggcccagc gccagcaccg gcttctggag ctggatcacc accgtcgacc
     1681 acaagcggat cgggatcctc tacggcttca cggccttcac gttcttcctg atcgggggga
     1741 gcgaggccct gctgatccgg atccagctgg cccgtcccga ccagcagatc ctggatccga
     1801 acgcgtacaa ccagatcttc accatgcacg gcctgaccat ggtcttcctg gtggtcatgc
     1861 cgttgtcggc ggcgttcttc aactacctga tcccgctgat gatcggggct cgcgacgtcg
     1921 ccttccccag gctgaacgcc ttcagctact gggcgttcct gctgggcgga ctgttcatct
     1981 actccggctt cgccctgggc ggcgcgccca acggcggctg gttcgggtac gcgccgctgt
     2041 ccacggtgga gcccgggaac aacatgctgt tctacgcgat cgggctgatc atcaccggcg
     2101 tcgcctccac cgtctctgcg gtgaacctgg ccgtcaccat catcaacatg cgggcgccgg
     2161 ggatgtcgct gttccggatg ccggtgttcg tgtggatggc gctggtggtc cagctgctcc
     2221 tggcgttctc gctgccgatc atcaccgttg ggctcttcga gatgctgttc gatcgccggt
     2281 tcggcaccca cttcttcgac ccggcggcgg gcggtgaccc ggtgctgtgg cagcacctgt
     2341 tctggttgtt cggccatccc gaggtctaca tcctgatcct gcccgccatg ggcatcgtct
     2401 cggagatcct gccggtgttc tcccgcaagc cgttgttcgg ctaccagttc gtggtcttca
     2461 gcggcatcgc gatcgggttc atcgggttcg gcgtgtgggc gcaccacatg ttcaccagcg
     2521 ggctcggccc ggtggccaac acggcgttcg ggatctccac catgatcatc gcggtcccca
     2581 ccggggtgaa gatcttcaac tggctcggca cgatgtggcg cggcgacatc cggttcacca
     2641 cgcccatgct gttcgccgtg gcgttcatcg ggcagttcgt ggtgggcggc ctgtcggggg
     2701 tgacccatgc cgtggtcccg gccgactacc aacagaccga cacctactac gtggtggcgc
     2761 acttccacta cgtgctgttc gccggcgcga tcttcgggct gttcggcggg atgtactact
     2821 ggtggccgaa gatcttcggc cgcaggctga acgagaggat gggcaaggtc cagttctggg
     2881 ccatggtgat cggcgcgaac ctcaccttcg cccccatgca catcctgggc ctgcagggga
     2941 tgccccggcg gatccagacc tatccggcct cgctgggcct ggggttctgg aacttcctgg
     3001 tgagcatcgg ggccttcatc atcgcggcga gcgtgctgct gttcatcctg aacgcggccg
     3061 cgagcttccg ccgccccaag gacgccgagg ccgacccctg ggacgcgcgc accctcgagt
     3121 ggatgacctc ctccccgccg cccgcgcaca acttcgacga gatcccggtg gtgcattcgc
     3181 tggacgagct gtggcaccgt aagtacgcgg agtccgaggg cggcgccgtg gttccggtcc
     3241 ccgcgggggg atcgggcgag caggaggtcg tcacgggcaa ggcacacgag ggcggcgggc
     3301 acgccatcca catgccgagc ccctcctact acccgctcgt ggcggccctg ggctttcccc
     3361 tgatcgggta cgggatcatc ttccattggt ggatggcctt cgccgggatg gtgctgatcc
     3421 tggccggact gtacgggtgg gccctggagc cctcggcgga gtaggagcga tcgtggcact
     3481 gtccgaacac ctcttcgaga gcgagcacga ggccaccagc accggcctgc ccaacacgaa
     3541 gctcgccatg tggatcttcc tggcctcgga ctgcctgctc ttcggcgcgc tgatcaccac
     3601 ctacgtcctg taccggggcg ccagcgtggt ggggccgtac cccaaggacg tcttcgacat
     3661 cccctacacg tcggtgagct cgttcgtgct gctggccagc tcgctgacca tggtgctggc
     3721 gctggcggcg atccagcggg gcgacctggg gaggatgcgg gtgtggctcc tggccacggc
     3781 cctgctgggg atgacgttcg tgggcgggca ggtctacgag ttcaccagct tctaccgcga
     3841 ggggctcgga ctgtccacga acctgttcgg caccacgttc tacgtcctca ccgggttcca
     3901 tgggacgcac gtggggatcg gggtcctcat gctgctgtcc ctggtgtgga tgtcgttcac
     3961 cggcaagctc accaccaggg atccgatgac cgtggagctg gtggggctgt actggcactt
     4021 cgtggacgtc gtgtggatcg tgatcttcac ggtcgtgtac ctgatcccga cgggcaagta
     4081 gggggagagc gcgtggagag cacgcacgag ctgcaacagc agggagcgca tccggcaccg
     4141 atctcgggcg gccacaccgt ccggcacccg agccccaagg agtacatccg catcgcgctg
     4201 atcctggggg tggtgacggc cggcgaggtg acggtctact acgtcgagtc ggtccgctcc
     4261 atgctgatcc cgctgctgtt cctgttcgcg gcggtcaagt tcagcctcgt ggtgctgtgg
     4321 ttcatgcacc tgcgcttcga cagccggacc tacgcgcgct tcttcatgat gggcctggcc
     4381 ggtgcggtga ccctgttcat catcgtgctg ctgaccttca gcgcgttctc gaggtaggta
     4441 cgttgatcct gccccattgg cacccgcatc cggacgtctg gctcgtgctg ggggggacct
     4501 gggccgcgta cgtgatggcc gtccggcggc accggggccg ggtgccggcc gacgatcgga
     4561 acgaacggcg gcgcctgtgg atgttctccc tgggcatgct caccctgtgg atcgcctcca
     4621 cctggccgat ccacgacctg gccgaggact acctgttctc cgtccacatg atccagcacc
     4681 tcctgtacat gctcgtcgct ccccccctgt tgatcgccgg ctcgcccccg tggctgctgc
     4741 ggcggatcct tgggccgacc cccgtgcggg tggtggtgcg gttcctggtc cgcccggtgg
     4801 tggcgttcat cctcttcaac gccgtgctgc tgttcatcca ctggcccgcg gtggttgcgg
     4861 cgcagctgga ttcggagctc gcgcacttcg ggatccacct gctcacggtg agcgtctcgc
     4921 tggtgatgtg gtggccggtg atgtccccgc tgccggagat gccggcgctg tcgcctcccg
     4981 gccagatgct gtacctgttc ttccagtcgc tggcgcccac gatccccgcg tcgttcctga
     5041 cgttcgggac ctccatcctc tacccggcgt acgccgcgtt ccccaggatc tgggggatct
     5101 cggtccactc ggaccagctg gtggccggcc tgatcatgaa gctggggggc gggttggtgc
     5161 tgtgggggtt cgtggcgacc atcttcttcc gctggcacgc ccgtgaggag cgggacggct
     5221 gggatgccct ccagctgcgt cacgtggagc gcgaggtgcg atcggagctg accaagccat
     5281 gagatcgacc acccgagacc ggctcctcct gcccgtgctg atgccgatcg ggatcctggt
     5341 cgtgatcgcg gcggtcctgt tcggcttctc gcggatcctg ctgtccgtgt cccacggcgc
     5401 ggccaccgcc accgcactgg ccgtggcctt cggcatcatg gtggtctccg gcgtggtggc
     5461 gggccgggcc gtggtcaagc cctcccacct ggcctcgatg gtgggggcca tcgcgggcgt
     5521 cgcgatggtg gcggggggca tcgcgctggt caccatcgcc ccggccacct ccgagggtgg
     5581 gggcagcgcc gccacgctgc acctggtggc caaggacatc gcgttcgacc agaccaagct
     5641 ctccgccccg gccggcaagg gcttcaccat cgcgttcgac aaccaggacg cgggcctcca
     5701 gcacaacgtc cagatcttcg ataacaagga cttcagcggc gatccggtct tcgacggcga
     5761 gctgaccacg gggccggagc agatcgacta cgaggtccct gcgctcgacg cgggcacgta
     5821 ctacttccgc tgcgtggtgc acccctcgca gatgaccggc gagctggatg tcgtggaggg
     5881 cggtggcgga gcgggcggcg gtggacccac cgtgaccgtg tcggcgaagg acatccagtt
     5941 cgacacgtcg cagatcgagc tgcccgccga cacgccggcc acgatccact tccagaacga
     6001 cgacgccggg atccagcaca acatcgccat cttccccagc gagaaggacc tgcaggaccc
     6061 cgtgttccgt ggcgacctgg tcaccgggcc gggggcggtc gactaccaga tcccgccgct
     6121 gaagcctggc acgtactact tccactgcga cgtccatccc accatgaacg ggaaggtcgt
     6181 ggtgtccggg gccggcggcg ggggtggccc aagcgccacc ccctccgggg gcggaggtgc
     6241 gggtggcggc ggtggggggc ccaccaccac atcggtgacc gccgagggca tcaagttcgg
     6301 catctcccag atcgacctgc cggccaacca ggcggtgacg ctgaccttcg agaacaagga
     6361 cgccgggatc cagcacaaca tcgcgatctt caagagcgag agcgacctgt cgaacccgct
     6421 cttccggggc gaggtcatca cggggccggc cacggtccag taccagatcc cggcccttcc
     6481 ccccggcacg tactacttcc actgcgacat ccatcccacg atgaacggca cggtggtcgt
     6541 gggctgagcc gcccgcggcc cgttcgcgcg gccgcgtggg agcatggagg tcgatgcgac
     6601 gcctcctccc gctggtgctc ctcctcccgt tgctggcgtc gtccgcctgc acctcctctg
     6661 ccccggacgg gccgaccgtg acctcgatct cgggacccat gcccaccctg tccggcacga
     6721 ccctggttgg ccccgagctg tcgcccgacg agtacgccgg ccggccggtg gtggtgaact
     6781 tctgggccac gtggtgcggt ccgtgccggc gggagcagcc ggtgctctcc gcggcggagc
     6841 gctcggccgg aggggacggg ccggtcttcc tgggcgtcaa ctaccgggac gacccggcgg
     6901 ccgcacgggc ctacctgaag gagttccacg tggggtaccc gagcctggag gacgcgtcgg
     6961 gggacctggc ctaccggttc ggcgtccccg gtctccccgc cacgatcttc gtggatgccg
     7021 gcgggcggat gcgcttccgg gtgctggggg cgctcgacgc ctccaccctc tccgacctg
//
LOCUS       DASQQQ010000084         3194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_33245, whole genome shotgun sequence.
ACCESSION   DASQQQ010000084 DASQQQ010000000
VERSION     DASQQQ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33245"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VE646_03490"
     CDS             complement(<1..671)
                     /locus_tag="VE646_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HZD79091.1"
                     /translation="MKRDYYDILGIPRDADSQAIKKAYRQLALKYHPDRNPEDPDAED
                     RFKEATEAYEVLRDSELKALYDRYGHEGLQAGVRGGGGGFGGFRTFDEALNIFMREFG
                     GFGFEDFFGTGRRRGARVRGSGVKIRVRLTLEEVTKGAKKTVRIPILDTCGDCGGTGG
                     RDGAEPSACPTCGGAGEIQQVQRSLFGQVVRVGPCAACGGEGKIIRDPCPTCSGEGRQ
                     RVAVDR"
     gene            complement(1082..2350)
                     /gene="dprA"
                     /locus_tag="VE646_03495"
     CDS             complement(1082..2350)
                     /gene="dprA"
                     /locus_tag="VE646_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZD79092.1"
                     /translation="MNEAAPLADLLAACETPSLALRAPRSVWRTVCGEDVPEPERLAE
                     AARRAEEDIRALEAFDAAIVDFRQADYPRLLRPLPQPPAMLFVRGRAEVLDWPGVAVV
                     GTRGCTRYGRDTAAAISLGLAGAGYAVISGLARGVDAAAHRAAAEAGAATIAVLGTGI
                     DVTYPPEHAGLARKILEIGCLVSEFPPGTPPRKYHFPQRNRLIAGLARAVVVVEADER
                     SGALITASHALEQGKDVFAVPGAIDARTSRGTNRLIQDGAGLVTCAADVTEALGSWAW
                     EGHREGSSFGESSDLGVPGGPSAVESATLTPRLGAGGAAAGGRPRGQAGGGSGVGDEA
                     AIAPPMREADARLRFDAAGREGSEPPPGSRGVARRVLQAISQGIDDLDSLAAALGEGT
                     GLLSGVLLELEVAGLVERLPGPRFRSVGRR"
     gene            complement(2575..>3194)
                     /locus_tag="VE646_03500"
     CDS             complement(2575..>3194)
                     /locus_tag="VE646_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZD79093.1"
                     /translation="TNRAPREHESGEAGEERRIELELKLIADVGLVGQPNAGKSTLLA
                     SLSAARPKIAEYPFTTLEPHLGVVDLGGGRTLVVADIPGIIEGAHEGKGLGLRFLRHI
                     ERTRTLAYLVPVDAEDPQAEYELLRSELQEYSGELARKPHCVVLSKADLVPPDGEAPS
                     LGAPDAFGVYAVSGVTRKGLQELAEALWGEVMKYVDSEASHGGTG"
BASE COUNT          475 a         1192 c         1026 g          501 t
ORIGIN      
        1 cggtcgaccg ccacgcgctg ccgcccttcg ccggagcacg tcgggcaggg atcccggatg
       61 atctttccct cgccgccgca cgccgcgcag ggacccaccc gaacgacctg cccgaacagc
      121 gaacgctgaa cctgctggat ctcgcccgcc ccgccacaag tggggcaggc gctgggctcc
      181 gcaccgtccc gcccgcccgt gcctccacag tcgccgcagg tgtccagaat ggggatgcga
      241 accgtcttct tcgccccctt cgtgacttcc tccagcgtca gccgcacgcg gatcttgacg
      301 cccgagcccc gtacccgggc gcccctgcgg cggccagtgc cgaagaagtc ctcgaacccg
      361 aagccgccga actcccgcat gaagatgttg agcgcttcgt cgaaggtcct gaaccctcca
      421 aatccgccgc cgccgccccg cacgccggcc tgcagaccct catggccgta gcgatcgtag
      481 agcgccttca gctccgaatc cctgagcacc tcgtaggcct ccgtggcctc cttgaacctg
      541 tcctctgcgt cggggtcttc cgggttccgg tctggatggt acttcaaggc gagctgccga
      601 tacgccttct tgatcgcctg tgaatcggca tcacggggga tcccgagtat gtcgtagtag
      661 tccctcttca tctaagcccg tctacagatg ctacgcatcc cggccaaggg cctgacaccc
      721 tcggccagcg atgatcaatc tccagcgacc tattcaagtt cgcagaacgg ctcgggcttt
      781 tcaatcccac ggtttcggtg gggtcgggcg gcggtatccg gtattcggta ttcgtcggta
      841 ttcggtgttc ggtgttcggt gttcggtctt cggtggacgg tggacggtgg acggtgggcg
      901 gccggcggta gtcgacccac ggttggtcct ccaggggttg ggggtctctc agcggcaatg
      961 ctcccacctc ccgtccaccg accggcagca ccgccgactg acccaccgcc acccgatcca
     1021 ccgaaatccg ccacccgatc caccgaaacc accgcccgac ctaccgaaac cgccgcccga
     1081 cctaccgccg cccgaccgac cgaaaccggg gacccggaag tcgctcgacg agcccggcga
     1141 cctccagctc gaggagcacg ccggagagga gcccggtccc ttcccccagg gcagcggcga
     1201 gggagtcgag gtcatcgatg ccctgggaga tggcctgcaa cacgcgtcgc gccacccccc
     1261 ggctaccggg tggcggctca ctcccctctc taccggcggc gtcgaacctg agcctggcat
     1321 cggcctcgcg catcgggggc gcaatcgccg cctcgtcccc aacgccggag ccaccgccag
     1381 cctgcccccg gggccggccg ccggctgcgg cgccacctgc gccgagccgc ggggtgaggg
     1441 tggccgattc tacagctgac ggtccaccag gcactcccag gtcggaagac tcaccgaagg
     1501 aggatccctc ccgatgcccc tcccaagccc acgagccgag ggcctccgtc acatccgccg
     1561 cgcaggtgac cagccccgcc ccatcctgga tcagtcggtt ggtcccccga ctcgttcgcg
     1621 cgtcgatagc ccctggcacc gcgaacacat ctttcccctg ctccagcgcg tggctggcgg
     1681 ttatcagcgc tccgctgcgt tcgtcggcct ccacaaccac caccgcacgc gccagaccgg
     1741 cgatcagccg gttgcgctgg gggaagtggt acttccgggg cggcgtcccc ggcgggaact
     1801 cgctcaccag gcatcctatc tccaggatct ttcgagcgag gcccgcgtgc tcgggtggat
     1861 acgtgacatc gatcccggtt ccgagcaccg cgatcgtggc ggcgccggct tcggcagccg
     1921 cccggtgggc ggccgcatcg actccgcgtg ccaacccgct gatcacggcg tatccggccc
     1981 cggccaggcc gagggagatg gcagctgcgg tatcacggcc gtaacgggtg caaccgcggg
     2041 ttcccactac cgcgacgcca ggccagtcca ggacttccgc ccggccccgc acgaagagca
     2101 tggccggtgg ttggggcagc ggccgcagca gcctgggata gtccgcctgc ctgaagtcga
     2161 cgatcgcggc atcgaaggcc tcgagggccc ggatgtcctc ctccgcccgt cgcgctgcct
     2221 ccgccagccg ctccggttcc ggcacgtcct ccccgcagac ggtccgccag acggacctcg
     2281 gagcgcggag ggcgaggctc ggggtctcgc aggcggccag caggtcggcg aggggggcag
     2341 cctcgttcat gccgtcacgt ctgagggtga ggagggcgat ccactcgtcg cgtcgtgacg
     2401 gcatgaggac aggcctggcg cgctggcggt gtcaggctag ccgggcgcgg cgaccgcacg
     2461 gcgacggcct ggcgtggccg gggcatcgga tgagccggca cgccggcggc atagcggctc
     2521 cgtggcgtga tggctgcacg aggtcgaccg gtgatgaggt catgcggcgc ctggttaacc
     2581 cgttcctccg tggctcgctt cggagtccac gtatttcatc acctcacccc agagggcctc
     2641 ggcgagctcc tggagccctt ttcgggtaac gccggagacc gcgtagaccc cgaaggcgtc
     2701 gggcgcgccc agtgacgggg cctcaccatc aggcggaacc agatccgcct tcgacagcac
     2761 gacgcagtgc ggcttccgcg ccagctcgcc actgtattcc tgcagctcgc tccgcaagag
     2821 ctcgtactcc gcctgagggt cctccgcgtc gaccgggacc aggtaggcga gcgtgcgggt
     2881 gcgctcgata tgacgcagga agcggaggcc gagacccttg ccctcgtgag caccttcgat
     2941 gatgccgggg atgtccgcca ccaccagggt ccggccaccc cccaggtcca cgacccccag
     3001 gtgcggctcg agggtggtaa atgggtactc ggcgatcttg gggcgcgccg ctgacagcga
     3061 tgccagcaat gtgctcttcc ccgcgttggg ctgtcccacc aggccgacgt cggcgatgag
     3121 cttcagctcc agctcgattc gccgctcctc ccccgcctcc ccactctcgt gctcgcgagg
     3181 cgcccgattc gtgg
//
LOCUS       DASQQQ010000085         6990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_223895, whole genome shotgun sequence.
ACCESSION   DASQQQ010000085 DASQQQ010000000
VERSION     DASQQQ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223895"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VE646_03505"
     CDS             <1..301
                     /locus_tag="VE646_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79094.1"
                     /translation="LCEARLQLAVQDRTGTPSYLSTPAREPTGAMLRLLRHRDPECRF
                     PGCRTRAYLKAHHMAWVSRGGPTTLSSLVQPGADLHLPSPPGARVRVGPGPGGRR"
     gene            584..916
                     /locus_tag="VE646_03510"
     CDS             584..916
                     /locus_tag="VE646_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HZD79095.1"
                     /translation="MTELPEQPAMPESPVARDDRLDAARKRAEEIQGFYVHLIVYVVV
                     NAGLFTINALSRGDSGVWWFYWPLAGWGIGLLIHAATTYLGVFSEDWKERKARQLFER
                     EHHGSARA"
     gene            complement(938..2509)
                     /locus_tag="VE646_03515"
     CDS             complement(938..2509)
                     /locus_tag="VE646_03515"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HZD79096.1"
                     /translation="MLLSDLVRASSRVAATRARSAKIEALASVLGRARVAEVPVAVAY
                     LSGSLPHGSIGVGWATVRNLPSPTGSEPTLELGSVDRTLRRIGATTGSGSQAARRAEL
                     AQLFEAATEEEQRFLRGLLTGEIRQGALEGLMVEAVARASDVPAQAVRRALMLGADLG
                     AVARAALSEGERGLAGFRLRVLEPVQPMLAQSATDLEDALGRIRPASVEWKLDGARLQ
                     VHRAGREVRAFTRNLADVTSRVPEIVERVAGLPVEAVVLDGEAIALRDDGRPHPFQVT
                     MGRFGSRLAVEALRASVPLSCFFFDLLHLDGEDLIDRPLAGRLTVFDERLPQEVRIPR
                     IVAASAAEAEPFLEGTLAAGHEGVMVKSLEAPYGAGRRGAGWLKVKRAHSLDLVVLGA
                     EWGHGRRRGWLSNLHLGARDPETGGFVMLGKTFKGMTDQVLEWQTERLLQLETRRDRF
                     TVYVRPGLVVEVAFDGLQTSPRYPGGLALRFARIKGYRHDKAPEDADTIDTVRALHAG
                     DAGASAASGGQDAAR"
     gene            2588..3253
                     /locus_tag="VE646_03520"
     CDS             2588..3253
                     /locus_tag="VE646_03520"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZD79097.1"
                     /translation="MPTEPERCCFDEWAGSYARRARRRRLVGPSRDLLAGLSEVGLRD
                     RTVLDVGCSAGVLAFETLEGGASRVTGIDLSPAAIQEAGRIAGERGLADRATFQVADG
                     ASAPLVPHDVVVLDKVFCCYPNVDRLLANSLGAARALYAFSLPPSDGLRGALQRAFSA
                     VANRWYRLRRRTFGDFRTYVHDVAALEARVREAGFEHRFGRRRLGWDLAIYVRPESTV
                     SER"
     gene            complement(3273..3560)
                     /locus_tag="VE646_03525"
     CDS             complement(3273..3560)
                     /locus_tag="VE646_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79098.1"
                     /translation="MTVSVIVQFIVKVPDVDRFVAASEKYAPMMREMGARNSAAYEDE
                     NEPGLMSTLSEWDTHDQMHEASEKYGEGFNADAGTEGLDWVTHIWHRKGEA"
     gene            3866..3949
                     /locus_tag="VE646_03530"
                     /pseudo
     CDS             3866..3949
                     /locus_tag="VE646_03530"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase"
     gene            3964..4215
                     /locus_tag="VE646_03535"
     CDS             3964..4215
                     /locus_tag="VE646_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79099.1"
                     /translation="MEQRVRHRQRRGGDLPPDRRRHHRVMPQMRFFPQDWAERSAGAV
                     GLVGHQVREDLGRFKTFIEGRGEETGAWRGEIPRQRRTG"
     gene            complement(4227..4430)
                     /locus_tag="VE646_03540"
     CDS             complement(4227..4430)
                     /locus_tag="VE646_03540"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZD79100.1"
                     /translation="MSAHCGRSRAWATWRSSQLAVHLRSSLKAGVRGDELKEIILQVA
                     TLAGFPPAMNAMSTLKTVLSESR"
     gene            complement(4876..5652)
                     /locus_tag="VE646_03545"
     CDS             complement(4876..5652)
                     /locus_tag="VE646_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010988112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD79101.1"
                     /translation="MTTAIRHTWYMTVRHLRALFRQPVWIMITLVQPIIWIVLYGALF
                     KNITELGGFGTNDYIVFLAPGIVVMTALFSGGWSGMALIEDIDRGVVDRFLVTPVRRG
                     ALISGRLVQLGLVAVIQSLILIGIAFVLGARWDGGALGLLVLVGCSILLGAAFGALSN
                     AMAVVVRKEETVIAAVNFVILPLTFLSSVFLPTSQAPGWIRTAATFNPVNWAVVAGHA
                     ALSAGTDWAAVMARTGYLLSFALVCAWLATSAFRAYQRSV"
     gene            complement(5649..6653)
                     /locus_tag="VE646_03550"
     CDS             complement(5649..6653)
                     /locus_tag="VE646_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014181065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZD79102.1"
                     /translation="MTDAIEAEGLIKTYPGGVRALDGIGFGVEAGTVFGLLGPNGAGK
                     STTVKILTTLSRPDAGRAGVEGFDVLKSPDRVRRVIGVVAQRSGVDREATGRENVRLQ
                     GQLYGMGGKGLERRVDELLERFGLSEAADRVARGYSGGMQRRLDIALGLVHRPRVLFL
                     DEPTTGLDPEVRAGMWGMITRLAADEGLTILLTTHYLEEADRLARRLAIVDQGRIVAR
                     GTPEQLKAELRGDAIQVELADVQANGDVHEALGPLVEVREVVVDGRALRARADDGARA
                     VPVVLQALESRGVGVTSVRVSRPSLDDVYLRHTGRSFTEADRAGEAPQPDDRTKEAQR
                     "
     gene            6776..>6990
                     /locus_tag="VE646_03555"
     CDS             6776..>6990
                     /locus_tag="VE646_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005832633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZD79103.1"
                     /translation="MIAEGRAAQTASDVLDEAAVGVMGINRTDGRCLEIMERSGGLTA
                     GRLASESGLTTGAVTAVLDRLEQAGYVR"
BASE COUNT         1035 a         2600 c         2367 g          988 t
ORIGIN      
        1 cctctgcgag gccaggctgc agctggcggt gcaagaccgt acgggcaccc cgagctacct
       61 gtccaccccg gcccgggagc ccaccggggc gatgctgcgg ctgctgcggc accgggaccc
      121 cgagtgccgc ttccccggct gtcggacccg ggcctacctg aaggcccacc acatggcctg
      181 ggtgagccgg ggtgggccca ccaccctttc cagcctggtc caacctggtg ctgatctgca
      241 ccttccatca ccgcctggtg cacgagtacg ggtggggcct ggtccgggcg gacgacggtg
      301 aactccgctg gtaccggccc gacggggtcc ggtaccgagc cgggccggta ccagctctcc
      361 gacctccaga gaccctggct ggctgaccgg ccggtcatga acacctgacc ctgcctaccg
      421 gtccaccgag cctgatctgc gtgccgccgt cgccttgctg ctgtgaccgc atggcatcct
      481 tcccgccctc gattcccggc ctcggtcccg tccgacgagt gcccaacgac ccctgcgacg
      541 tgcggggtgg gatgtgagcc tcagtggaac ggaggtgatc acgatgaccg agcttccaga
      601 gcagccggcc atgcccgagt cgccggtggc gcgtgacgat cggctcgacg cggccaggaa
      661 gcgggccgag gagatacagg ggttctacgt ccacctcatc gtctacgtgg tggtgaacgc
      721 cgggctcttc accatcaacg cgctcagtcg cggcgacagc ggcgtctggt ggttctactg
      781 gccgctcgcc gggtggggga tcggcctgct gatccacgcg gcgacgacct accttggcgt
      841 cttctccgag gactggaagg agcgcaaggc tcgtcagctc ttcgagcgcg agcaccacgg
      901 ctcggcccga gcctgagccc atcgggcagc cggaggctca tcgggcggca tcttgcccgc
      961 cgctcgccgc tgaagcaccg gcgtcgcccg cgtgcagcgc ccggaccgta tcaatcgtgt
     1021 cggcgtcctc cggcgccttg tcgtggcggt agcccttgat ccgagcgaac cgcagcgcga
     1081 gcccgcccgg atagcgcggg ctggtctgga gcccgtcgaa ggccacctcc accaccagcc
     1141 cgggacgcac gtagacggtg aagcggtccc gtcgggtctc gagctgcagc agtcgctcgg
     1201 tctgccactc gagcacctgg tcggtcatcc ccttgaaggt cttgcccagc atcacgaacc
     1261 cgccggtctc cgggtcgcgc gccccgaggt gcaggttgct gagccacccg cgtcggcggc
     1321 cgtggcccca ctcggccccc agcaccacca ggtccagcga gtgcgcgcgc ttcaccttca
     1381 gccaaccggc cccccggcgc ccggctccgt acggggcctc cagggacttc accatcacgc
     1441 cctcgtgccc cgcggcgagg gtcccttcca ggaacggctc ggcctccgcc gccgacgccg
     1501 ccacgatccg cgggatccgg acctcctgcg gcaggcgctc atcgaagacg gtgagccgac
     1561 cggccagggg tcggtcgatc aggtcctcgc cgtcgaggtg caggaggtcg aagaagaagc
     1621 acgacagcgg caccgacgcc cggagcgcct ccaccgccaa gcggctcccg aaccgcccca
     1681 tcgtcacctg gaatggatgc ggcctgccgt catcgcggag ggcgatcgcc tccccgtcca
     1741 gcaccaccgc ctccaccggg agacccgcga cccgctcgac gatctccggg acgcgcgagg
     1801 tgacgtcggc caggttccgg gtgaacgccc tgacctctcg cccggcccgg tgcacctgca
     1861 gccgtgcccc gtccagcttc cactccaccg aggcggggcg gatcctcccc agggcgtcct
     1921 ccaggtcggt cgcgctctgc gcgagcatcg gctgcaccgg ctcgagcacc cgcaggcgga
     1981 agccggccag tccccgctcg ccctcggaga gcgcggcccg tgccacggcc cccaggtcgg
     2041 cgccgagcat cagcgcccgc cgaaccgcct gcgccggcac gtccgaggct cgggccaccg
     2101 cctcgaccat caggccctcc agcgcgccct gacggatctc cccggtcagc agcccgcgga
     2161 ggaagcgttg ctcctcttcg gtggcggcct cgaacagctg ggccagctcc gccctgcggg
     2221 cggcctgcga ccccgagccc gtggtcgcgc cgatccgtcg gagcgtccgg tccaccgacc
     2281 cgagctccag cgtgggctcg gagccggtcg gggaggggag gttgcggacc gtggcccatc
     2341 ccacgccgat cgacccgtgc ggcagggagc cagagaggta cgccacggcc accggcacct
     2401 cggcgacccg tgcacgaccg aggaccgagg cgagcgcctc gatcttcgcg gatcgggctc
     2461 gcgtggcggc cacccgggac gaggctcgga ccagatcgct cagcagcatg ggggcaggat
     2521 ggcagaccgg accgccggcc gacccacgct tgacgctcgg gcggttctgg tgccaccgtc
     2581 cggagtgatg cccaccgaac ccgagcggtg ctgcttcgac gagtgggccg gctcctacgc
     2641 ccgtcgggcc cggcggcgcc ggctggtcgg cccctcccgt gacctgttgg ccggcctctc
     2701 cgaggtcggc ctgcgggacc ggaccgtgct ggacgtcggc tgcagcgcgg gtgtgctcgc
     2761 cttcgagacg ctcgagggtg gagcgagccg ggtgacgggg atcgacctat caccggccgc
     2821 gatccaggag gccggtcgga tcgccgggga gcggggactg gccgatcgag ccacgttcca
     2881 ggtggccgat ggggcctcgg ctccgctcgt cccacacgac gtcgtggtgc tcgacaaggt
     2941 gttctgctgc tacccgaacg tcgaccggct cctcgccaac tcgctgggcg ccgcgagggc
     3001 cttgtacgcc ttctccctcc cgccctccga tggtctccgg ggcgcgctcc agcgcgcctt
     3061 ctcggccgtc gccaaccgtt ggtaccgcct ccgcaggcgc acgttcggcg acttccgcac
     3121 gtacgtgcac gacgtggccg cgctcgaggc tcgggtccgc gaggccggct tcgagcaccg
     3181 cttcggccgc cgccgtctcg ggtgggacct ggcgatctac gtccgtccag agtcgaccgt
     3241 ctcggagcgc tgagccgaag gacggggggc ggttacgcct cgcccttgcg atgccagatg
     3301 tgggtgaccc agtccagccc ttccgtgccg gcgtccgcgt tgaacccctc gccgtacttc
     3361 tccgaggcct cgtgcatctg gtcgtgggtg tcccactcgg acagcgtcga catcagtccc
     3421 ggctcgttct cgtcctcgta cgcggcggaa ttccgcgccc ccatctcccg catcatcggg
     3481 gcgtacttct cggacgccgc tacgaaccga tcgacgtcgg ggaccttcac gatgaactgc
     3541 acgatgaccg acacggtcac cacctccctt cccagggtgg aagagcgtgc catgcggtca
     3601 tggagggcgc aaggcgcgag cgccgagccc gtagctgcgc cctggtctcc ttccgcggtc
     3661 ggacccatgt tgcccccgac gagccggtgg gtacggcgga tagcgagagg catcccaccc
     3721 cataggaggt tcccatgccc gcgcacgagg aacacacgta caaggagtcc gtcgaggtcg
     3781 cggttcccct cgccgccgcc tacaccagtg gacgcagttc gaggacttcc cggccttcat
     3841 ggacggggtc gagtcggtcc agcagatggc ggaggaccgg ctccactggg tcgccctgat
     3901 cggcggccgc cggcgcgagt gggacgccaa gatcaccgag cagaagcccg atgagcggat
     3961 cgcgtggagc agcgagtccg gcaccggcaa cgccggggtg gtgaccttcc accggatcga
     4021 cgacggcacc accgggtcat gccccagatg cggttcttcc cgcaggactg ggccgagcgg
     4081 tcggccggcg cggtcggcct ggtgggacat caggtccgcg aggacctggg ccgcttcaag
     4141 accttcatcg aggggcgggg cgaggagacc ggggcctggc ggggcgagat cccccggcag
     4201 cgacggaccg gctgacgcgc gtcggcctaa cgtgactcgg acaggaccgt cttcagcgtc
     4261 gacatcgcgt tcatggccgg ggggaaaccc gccaacgtcg ccacctgcag gatgatctcc
     4321 ttcagctcgt cacccctcac tccggccttc aacgaggacc ggaggtggac ggcgagctgg
     4381 gaggaccgcc aggtcgccca ggccctcgat cgtccgcagt gagcgctcac cgtggatctc
     4441 cagcaggcgt cgacgcccgt tcgagccggc cgcccatccc tcgctccttc atcccggcgt
     4501 gatcccaggc ggggaagcta tcccaggacg gggcgatcgg gtccgctcag atcacgccga
     4561 tcacgcggag gatgccgaag gtggcggcgg cgcccaccag cacgatgccg atcccgacct
     4621 cctgcttccg ctcggggatc ccttcctgga cgccaggacg gccatccgcc agttggaccg
     4681 ggccgccccg gccgccagta gcagcgaggc ctctgtcccc tcgaccgagt tcaggaacag
     4741 cggcaggatg aacccgggga tcccgccacc ggagaagacc gcgaggagcc gaaccacgtc
     4801 cccacggttc ccgccgcccg acgaggacga acagggagcc gcggaccctc gcggggatcc
     4861 cgccgcgcga tcctcctaga ccgatcgctg gtaggcgcgg aaggcgctcg tggccagcca
     4921 cgcgcagacc agcgcgaagc tcagcaggta cccagtgcgt gccatcactg ccgcccagtc
     4981 ggtcccggcc gagagcgccg catgcccggc caccacggcc cagttcaccg ggttgaacgt
     5041 ggcggcggtc cggatccagc cgggggcctg gctcgtgggg aggaacaccg atgacaggaa
     5101 ggtgaggggc aggatcacga agttcaccgc cgcgataacc gtctcctcct tgcgcacgac
     5161 gaccgccatc gcgttcgata gagccccgaa cgcggccccc aggaggatcg agcatcccac
     5221 cagcaccagc agccccagcg ccccgccgtc ccaccgggct cccaggacga acgcgatgcc
     5281 gatcaggatc agcgactgga tcacggccac cagacccagc tgcaccagcc gccccgagat
     5341 cagggcccca cgtctgaccg gggtcaccag gaaccgatcc accacgccac ggtcgatgtc
     5401 ctcgatcagc gccatcccgc tccacccgcc ggagaacagc gcggtcatca ccacgatccc
     5461 cggagcgagg aacacgatgt agtcgttggt cccgaacccg ccgagctcgg tgatgttctt
     5521 gaacagggcg ccgtagagca cgatccagat gatgggctgg acgagcgtga tcatgatcca
     5581 gacgggctgg cggaacaggg cgcgcaggtg acgaaccgtc atgtaccagg tgtgtcggat
     5641 cgccgtggtc atcgctgcgc ctccttcgtg cggtcgtcgg gttggggtgc ctcaccggcg
     5701 cgatctgcct cggtgaacga gcggccggtg tggcggaggt agacgtcgtc cagcgatggg
     5761 cgggagaccc tcaccgaggt caccccgacc ccccgggact cgagtgcctg gagcaccacc
     5821 ggcacggcgc gagctccgtc atcggcgcgg gcccggagcg cccgaccgtc cacgaccacc
     5881 tcgcggacct cgaccaacgg gcccagggcc tcgtgcacgt cgccgttcgc ctggacatcg
     5941 gcgagctcca cctggatcgc gtctccgcgc agctccgcct tgagctgctc gggggttccc
     6001 cgcgccacga tgcgaccctg gtccacgatg gccaggcgcc gggccagccg gtcggcctcc
     6061 tccaggtagt gggtggtgag caggatcgtg aggccctcgt cggccgccag gcgcgtgatc
     6121 atcccccaca tcccggcccg gacctccggg tccagtcccg tggtcggttc gtccaggaac
     6181 aggaccctgg gccggtggac caggccgagc gcgatgtcca gacggcgctg catcccgccc
     6241 gagtacccgc gggccacccg gtccgcggcc tccgagagcc cgaaccgctc caacagctcg
     6301 tccacgcgac gctcgaggcc cttgcccccc atgccgtaga gctgcccctg gaggcggacg
     6361 ttctcccggc ccgtggcctc tcggtccacg ccggagcgct gcgccaccac gccgatcacc
     6421 cggcggacgc ggtcggggct cttcagcacg tcaaagccct ccaccccggc tcgtccggcg
     6481 tccgggcggg acagcgtggt gaggatcttc acggtggtcg acttgccggc gccgttgggg
     6541 ccgaggagcc cgaacaccgt gccggcctcc accccgaagc cgatcccgtc cagcgcccgg
     6601 accccgcccg ggtaggtctt gatgaggccc tcggcctcga tcgcgtcggt catcctcgca
     6661 cctctcttcc cgcccccggc cccggaccgg tggtagtatc cgttcgagtg gtgagtatct
     6721 tatgagtcaa gagactgaat cgtcaagagg gattcgggac cgtctcgtcg gcgaggtgat
     6781 cgccgagggc cgggccgctc agaccgccag cgacgtcctg gacgaggcgg ccgtaggggt
     6841 catgggcatc aaccggaccg acggccgatg cctcgagatc atggagcgca gcgggggcct
     6901 cacggccgga cggctcgcct cggagagcgg tttgaccacg ggtgcggtaa cggcggtcct
     6961 ggaccggctg gagcaggccg ggtacgtacg
//
LOCUS       DASQQQ010000086         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_88310, whole genome shotgun sequence.
ACCESSION   DASQQQ010000086 DASQQQ010000000
VERSION     DASQQQ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88310"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VE646_03560"
     CDS             complement(<1..655)
                     /locus_tag="VE646_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZD79104.1"
                     /translation="MGPDPGRIAELKGREDERFRAEHPRSLELLARARASMPNGVPMA
                     WLADPGTYDHPPVWALDGHGARFRDVDGHEYVDLNIADMSMFCGYTPPQVVEAVARRM
                     AAGNQFLLPVEDAIWVSEELARRWSLPKWQFTLSASQANTEVIRVARVATGRPRAVMF
                     EGKYHGHFDQALVALRDGHEVPEEPGLPPGVTDQTRVIPFNDVAALDRALDPGDVAVV
                     "
     gene            692..2206
                     /locus_tag="VE646_03565"
     CDS             692..2206
                     /locus_tag="VE646_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HZD79105.1"
                     /translation="MPDPFSWHPEPELIGFRDPARSRAALEELGRAAWEASLDYLYEE
                     AMRRPVAPESYPDLRRWFFGPSNEPAPAPLEPAPWKQVLAQFRERVAPHVFNSQHPGS
                     FSYFTPPPLPASIAGEVLSQWVHQGVDVWAAGPVAALVEEEVTAWLRDLVGLGAEGWG
                     VLTSGGVMANIMAINVIRDVHLPRVLDLRDQPRGRDLEGVRVYASDQAHFSIERALST
                     LGFPTDTLRIVPSDERFRLRGDPVARAVAEDRAAGRTPLAIAADSGSTNTGSVDLVGE
                     LAEVARAEGMWLHVDAAYGGAARLSDRDAGRVPDLERAHSVTIDPHKWFFQAYDVGAL
                     VVRRREDLLRTFHREPEYYRYPKPEDAPLNWMQYSIEGTRRFRALKLWLSWKHLGSAG
                     LGRLIEANDDLAAHLARRCRESPDLEAVPTEPELSVVCFRHLPPGLGPDELDRYQDRL
                     QRALEVSGEAWVSTTRLRGRTYLRAGIVNYLSTAEDVDRMLDALRRLSPSVTQP"
     gene            2275..>2454
                     /locus_tag="VE646_03570"
     CDS             2275..>2454
                     /locus_tag="VE646_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79106.1"
                     /translation="MAATDRPLRGFSRSYLDLSMAHWRIAAVPLGTGPSATSESASPG
                     PSDETGVARRAEDGPV"
BASE COUNT          336 a          901 c          870 g          347 t
ORIGIN      
        1 gcaccacggc cacatccccg ggatcgagcg cccggtccaa cgccgccaca tcgttgaacg
       61 ggatcacccg ggtctggtcg gtcacgcccg gaggcagacc gggttcctcg gggacctcgt
      121 gcccgtcccg gagcgccacc agggcctggt cgaagtggcc gtggtacttg ccctcgaaca
      181 tcacggcccg agggcgaccg gtggccaccc gggccacccg gatcacctcg gtgttggcct
      241 gggaggccga cagcgtgaac tgccacttcg ggagcgacca ccgccgggcg agctcctcgg
      301 agacccagat cgcgtcctcg acggggagca ggaactggtt gccggccgcc atccgtcgag
      361 ccaccgcctc caccacctgc gggggcgtgt acccgcagaa catgctcatg tcggcgatgt
      421 tgagatcgac gtactcgtga ccgtccacgt cccggaagcg ggcgccgtgc ccgtccagcg
      481 cccagacggg cggatgatca taggtgccgg gatcggcgag ccaggccatc ggcacgccgt
      541 tcggcatgga ggcccgggcc cgagccagca gctccaggga gcgggggtgc tcggcgcgga
      601 accgctcgtc ctcgcgcccc ttcagctcgg cgatcctgcc ggggtccggg cccatgggtc
      661 acccagccta gcggttgcta cagtccgccc cgtgcccgac ccgttctcct ggcatccgga
      721 gcccgagctg atcggcttcc gcgacccggc ccgctctcgg gcggcgctgg aggagctggg
      781 gcgggcggcg tgggaggcct ccctcgacta cctctacgag gaggcaatgc gccggcccgt
      841 cgcccccgag tcgtacccgg acctgcggcg gtggttcttc ggtccgtcga acgagccggc
      901 ccccgccccg ctggagccgg ccccctggaa gcaggtcctg gcccagttcc gggagcgggt
      961 ggccccgcac gtgttcaact cgcagcaccc cggctcgttc agctacttca ccccaccgcc
     1021 actgccggcg tcgatcgccg gagaggtgct ctcgcagtgg gtgcaccagg gcgtggatgt
     1081 gtgggcggcc gggccggtgg ctgccctggt ggaggaggag gtcacggcct ggctgcgcga
     1141 tctggtgggg ctcggcgccg agggatgggg cgtcctgacc tcgggaggcg tgatggccaa
     1201 catcatggcc atcaacgtga tccgcgacgt gcacctgccc cgtgtgctgg acctccgcga
     1261 ccagccccgg ggacgcgacc tggagggcgt gcgggtctac gcgtcggacc aggcccactt
     1321 ctcgatcgag cgggccctgt ccacgctggg attccccacc gataccctgc ggatcgtccc
     1381 ctcagacgag cggttccgtc tacgaggaga cccggtggcc cgagccgtgg ccgaggaccg
     1441 ggcggccggc cgcaccccgc tggccatcgc cgccgacagc ggctccacga acacgggctc
     1501 ggtggacctg gtgggcgagc tggccgaggt ggcccgggcc gaggggatgt ggctccacgt
     1561 cgacgccgcg tacgggggcg ccgcccggct ctcggatcgg gacgccggtc gggtgccgga
     1621 cctggagcgg gcccactcgg tgacgatcga tccccacaag tggttcttcc aggcctacga
     1681 cgtcggggcc ctggtggtcc gccgccgcga ggacctgctg cgcaccttcc accgcgagcc
     1741 cgagtactac cgctacccca agcccgagga cgcgcccctg aactggatgc agtactcgat
     1801 cgagggcacc cggcggttcc gcgccctgaa gctgtggctg tcgtggaagc acctggggag
     1861 cgccgggctg ggacgtctga tcgaggcgaa cgacgacctg gccgcccacc tggcccgccg
     1921 gtgccgcgag agcccggatc tggaggccgt ccccaccgag ccggagctct ccgtggtctg
     1981 cttccgacac ctcccgcccg gcctggggcc cgacgagctg gaccggtacc aggaccggct
     2041 gcagcgggcg ttagaggtct cgggggaggc gtgggtgtcc accacccgcc tgcggggccg
     2101 cacctacctg cgtgccggca tcgtcaacta cctgtccacc gccgaagatg tggaccggat
     2161 gctggacgcc ctccgacggc tctcgccgag cgttacccaa ccgtaacgac tccgcccctc
     2221 gtccgaaaca ctccggcggg acgatgtgat ccaccgacgg gccaaggaga ggccatggcg
     2281 gcgacggatc ggcccctgcg cgggttctcg cgttcctacc tggatctatc gatggctcac
     2341 tggcggatcg cggcggtccc ccttgggaca gggccgagcg ccacgtcgga gtcggcttcc
     2401 ccaggcccct ccgacgagac cggggtcgcg cgccgcgccg aggacggtcc cgtc
//
LOCUS       DASQQQ010000087        17167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_390272, whole genome shotgun sequence.
ACCESSION   DASQQQ010000087 DASQQQ010000000
VERSION     DASQQQ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 17167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17167
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390272"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..922
                     /locus_tag="VE646_03575"
     CDS             215..922
                     /locus_tag="VE646_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZD79107.1"
                     /translation="MLERLRRDARALGALEDELRRSLYLFVRAGGRPVNREEAAAAVG
                     ISRKLAAFHLDKLVDRGLLTFTYERPPGRGGRGAGRSAKYYRPSDREVEVSIPERRYD
                     LLGSVLLRALRSREPGEAAGEAAGRAARETGFQMGRDERQGLGIPPAGPERTMALAVR
                     LLERTGFEPYEDEPGSIRLRSCPFQALAERDRDLVCGMNHCLIGGVVAGLGSEAVDVL
                     LDPLPGGCCVTLKAPAH"
     gene            complement(926..1423)
                     /locus_tag="VE646_03580"
     CDS             complement(926..1423)
                     /locus_tag="VE646_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HZD79108.1"
                     /translation="MRKRTSLIALTVALGLLGAACSGGSGTAASGTASPTGEPATSPT
                     LSPTATGGGGGGTITIGGQRANDHGTKDVSAQGQLEVQMNDFYFDPTIVKGTPGEQVT
                     IELKNESGVLHNFSIQGQSIDQDVSPGEHQTVTVTIPQSGQAVFFCKYHQSLGMVGAL
                     EPSSG"
     gene            complement(1690..2334)
                     /locus_tag="VE646_03585"
     CDS             complement(1690..2334)
                     /locus_tag="VE646_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HZD79109.1"
                     /translation="MATTTARDALRRRALRLEYATIAWNLGEAVLTIWLGLAAGSLAL
                     VGFGTDSVIEVFASGVVVWHVRPGHQIDRPDRTALALNLVAGAFLALALILAAASIRN
                     LAGGQRAGHSLAGITYLAVTALVMFGLAVAKRRTAAGLGSAPLASEASMTFLDGMLST
                     ATLAGLALNAGLGWWWADPAAALLVSIAALNEAHENRGEARDLQRSAGKAGPDR"
     gene            2486..2740
                     /locus_tag="VE646_03590"
     CDS             2486..2740
                     /locus_tag="VE646_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004572663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HZD79110.1"
                     /translation="MNEVEIEYCVPCGLLPTAEETAHTLLSTYGQRLGGLRLKPGHGG
                     VFRVSVDGTRVFDKSEDGYDVAAIVSRVDERLAPERSLRS"
     gene            2812..3285
                     /locus_tag="VE646_03595"
     CDS             2812..3285
                     /locus_tag="VE646_03595"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZD79111.1"
                     /translation="MSTYTISEVATRTGFSTPTLRYYEQIGLIPRPERTEVGYRVFTE
                     EHLRILDFIGRAKRLGLPLEEIAVLAEAWAKRDCRATREQLERLVETKLTQVRHLIGD
                     LIRFRDQLEEVHLELTDRPAPRRCGPDCGCEIEVNTIDRDEVRVLSLVSLSPTHG"
     gene            complement(3695..4912)
                     /locus_tag="VE646_03600"
     CDS             complement(3695..4912)
                     /locus_tag="VE646_03600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZD79112.1"
                     /translation="MTVRRPGVAERGAISIPPPMGPTQLGEGLVRRAPVGTRLAYLDN
                     LKTVLIAGIIASHAVMGYATFGSWTYQDVQETTLSELVEKVYALLYLLLGGLFLMALF
                     FLVSGLLTPGSLQRKGPTRFTLDRLLRLGVPLAVYTLLVWPLLEYALLGPFRHRGFWN
                     SVTNTDPVLDNGPVWFVGVLLLYSILYVAWRWRCPEGGPSERALGGPTLVLLALGVGI
                     SLFLVRLALPMDSNQPLNVKAWGWPEYLATFGLGIVAAGRGWLRDVPRAVSRCSGIAT
                     IAASLVVVAVAVTADLRGLGQDVYFGGWGGLALVWAMSEGILAVSGPIWVLSFVQRHL
                     NATGPLRQIMARGSYAAFMLQGPVIIALELTLRPAAIPGDIKALLVATLGIVGSFALA
                     WPLVTRTPLRRIL"
     gene            5497..6270
                     /locus_tag="VE646_03605"
     CDS             5497..6270
                     /locus_tag="VE646_03605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZD79113.1"
                     /translation="MEELSARGFFDALAGEYHLLLDDWWEAAETHGRIIGGLLREHGV
                     PPGEGLLDCTCGIGTQALPLARQGYRVLGTDLSAAAIARARKEAKERGIQVELAVSDV
                     RQVHRLVPSPVEAVIACDNSFAHLLTKDDLRAALRSVRACLAPGRLFLASIRDYDRLS
                     EERPTGVVPLTYPTGGPRRIVGQAWEWSESAEALLIRLFILREEHDDWSCSLWATRLR
                     AWRRHEVDSALGVAGFHDIVWHFPDDSGYYQPIVTARAM"
     gene            complement(6311..6769)
                     /locus_tag="VE646_03610"
     CDS             complement(6311..6769)
                     /locus_tag="VE646_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZD79114.1"
                     /translation="MEAAVRRACSVFEEMGDLGHLSGFLALLADVLADMGRFEEAESQ
                     AARAWSMSADSDVMDQILLRRARAKILAARGDLESAVRTGREAVELTHGIQDTDLQAG
                     VWMDLGPIFNEAGRQAEAIDLATQAVEMWERKGNLVRAARAREVLRSMSA"
     gene            6781..7152
                     /locus_tag="VE646_03615"
     CDS             6781..7152
                     /locus_tag="VE646_03615"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZD79115.1"
                     /translation="MRLIEDHSERVGYLLKERVFDVAILVDALRRIADAECVIDPTIV
                     SRLVGRRRRADALEALTPREREVIGLVAAGLSNSAIAEHLYVTERTVEAHTKQIFQKL
                     ALEADPGSNRRVLAVLAYLRS"
     gene            7286..7555
                     /locus_tag="VE646_03620"
     CDS             7286..7555
                     /locus_tag="VE646_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79116.1"
                     /translation="MRVNIESGREAEAAEFLKATVLPAVKQSPGLVAAYWWQGSEGGQ
                     GSSLAVFDSEEAARGAAAMASSGQVPMPDYVSMAGADVVEIQASI"
     gene            7831..7903
                     /locus_tag="VE646_03625"
     tRNA            7831..7903
                     /locus_tag="VE646_03625"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7864..7866,aa:Arg,seq:acg)
     gene            complement(7915..8568)
                     /locus_tag="VE646_03630"
     CDS             complement(7915..8568)
                     /locus_tag="VE646_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZD79117.1"
                     /translation="MSESAPEDPELKRAVFARFDRPCPERTVFTTNTSSLIPSMFADA
                     TGRPDRFAALHFHLPVWSANVVDVMPHPGTAPEVTELLLAFAPRIGQIPIHLRKEHHG
                     YAFNSMYEAMNREAIALAANRIALVEDVDRAWMGVTKMPVGPFGMLDDVGRGTVRKIT
                     SFWAQQLGDPQLEKNEKYLRGYVDTGRLGRKSGEGFYRYPEPAYRDPGFLEGRFPTS"
     gene            complement(8568..8834)
                     /locus_tag="VE646_03635"
     CDS             complement(8568..8834)
                     /locus_tag="VE646_03635"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZD79118.1"
                     /translation="MNIDDVRPVLIVGAGTMGQQIGLQCAMHGYEVVLHDVQPSALRA
                     RMQGIRSTADQIVAARLMDGSERDRALPLIGTARIMRPRPKRPT"
     gene            complement(8831..9277)
                     /locus_tag="VE646_03640"
                     /pseudo
     CDS             complement(8831..9277)
                     /locus_tag="VE646_03640"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
     gene            complement(9293..9895)
                     /locus_tag="VE646_03645"
     CDS             complement(9293..9895)
                     /locus_tag="VE646_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79119.1"
                     /translation="MREFLRRRDEHAWSDLRAGALTGLLSSTYSTLFVQFLARDTGRD
                     PLVDWMQVGTVPLRNRAIRSRPGPVGVSAGILAHQLADFFWAVVFFGTIVPRVPRRRR
                     TAFLLATAALWAAATAAIEYYLALPWVQPILRMQVPYWTAMGVHLSSAAAYPLSMLFG
                     EGDRTVGRRAAALLGASIPMMAFIDVRSRTRRGLRWAIRG"
     gene            10219..10395
                     /locus_tag="VE646_03650"
     CDS             10219..10395
                     /locus_tag="VE646_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HZD79120.1"
                     /translation="MAAEDRARDALEMLDEEERQVLVRVAGILRRIDFGTVLLVVQDG
                     KVVQIEMAEKFRLR"
     gene            10532..11563
                     /locus_tag="VE646_03655"
     CDS             10532..11563
                     /locus_tag="VE646_03655"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZD79121.1"
                     /translation="MRKAPALLLVVALLTTGCASSASRAEGSSDVLRLGMFPNLTHAP
                     AYVALGEGIFDRDLAPTRVEVTYFNSGSDAQAAVTTGAIDATYIGPGPSITAYLQSGE
                     VAIVSGVASGGASFVIRKGSGISESADLHGKKVAVPGIGNTQDVALRTWLKDHGLRAK
                     DAGGDVAVAAIDNPELLQTFQAGHVDAAWEPEPWPSFLIQQGVARLFVDEATLWPRGR
                     FVVANLLVNTIFLKAHPHIVKRLVRANVEAIQAISDDPTHAQEAAQAGLIQAGAPSFD
                     QSVVDAAWSKLEFTWDPLPETLCKDARDAHALGYLEQDPKDISRIYRLGILNEVLKEE
                     GEPPVEVPA"
     gene            11560..12390
                     /locus_tag="VE646_03660"
     CDS             11560..12390
                     /locus_tag="VE646_03660"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD79122.1"
                     /translation="MRTLPRLRAVPDLETASPKLSARGVSKSFRSARGGTVPALVDID
                     LEIRRGEFVCLIGPSGCGKTTLLNLFAGLDAADQGDLRIDGAAITRPGPDRAVLFQEP
                     ALFPWLSVLANVEFALEMLGVPKDERRDRAMHWLHRVHLGRFADAQPHELSGGMRQRA
                     AIARALSCQPEVLLADEPFAALDAQTREILQGELQEVWSETRNTFLFVTHNVREAVFL
                     ADRVILMSAPPGTLVTEHRIEAPRPRDFEDILLSKVVVDIHAHLSREVEKVVAREVGP
                     "
     gene            12374..13162
                     /locus_tag="VE646_03665"
     CDS             12374..13162
                     /locus_tag="VE646_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD79123.1"
                     /translation="MRSVLSQLWPRLAAIGGLLLVWWLAYRSGLFNPAELPSPGQVIT
                     AFGDTFFKPHPPRESILAATQASLIRLCVGLAMGITVGTSIGLSMAVSSSVQRSVGSL
                     MSGLQALPSISWLPLAILWFGLTERAILFVVIIASIPAVAIAAASSIRLVPPLFVRAG
                     RTMGARGWTLYRRVVLPAAVPGYVGGLQSAWALGWRALMAGELISTGNKGLGHLLDAN
                     RQLLLTSNIFAVMLMIIVVGMAVEMTLGSIDRRVRARRGLVLAT"
     gene            13245..13442
                     /locus_tag="VE646_03670"
     CDS             13245..13442
                     /locus_tag="VE646_03670"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HZD79124.1"
                     /translation="MQRNRSILWALLLIAIGVVLLLRNSGTIAEDVRIWPILLMAVGA
                     WLLIERLAFGTRWGGGSARPW"
     gene            13439..14206
                     /locus_tag="VE646_03675"
     CDS             13439..14206
                     /locus_tag="VE646_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79125.1"
                     /translation="MILAIGVTFFLQDVGAISTDITIWPVVLIAVGVAIVLSAVPARG
                     RRAWGPVRETVPLDGAASARVVVKHGAGRLTVTSQVNPDLLVDGSFGAGVRKRVRRSG
                     DRLDVMLSHQGWWMEHARSWSGSGGPLDWEVGLSRLVPLELEMEGGAHRARLDLSDLQ
                     VRELRVETGASETTITVPSKGHCRARVEAGAASVTVRIPPHTAAQIRVQGGLSGVRVP
                     ETRFPAVPGGYRSPDFEEAADRVELEIQAGAAGITIE"
     gene            complement(14218..15549)
                     /locus_tag="VE646_03680"
     CDS             complement(14218..15549)
                     /locus_tag="VE646_03680"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HZD79126.1"
                     /translation="MGGGLFTDLYELNMAASYLRRGMNRTATFSLFVRRLPPERGFLV
                     AAGLEPCLEFLEGFGFEEEDLRYLREELGFDEPSLEAFRETRFDGEVWAVPEGRVLLA
                     DEPLLEITAPIAVAQLVETFLLNQISFQTAIASKAARCRLAARDRDVVDFAFRRTQGT
                     DAAMAVARTTAIAGFTATSNVEAARRYGLTATGTMAHSYIEAFPTEAEAFRAFAQDFP
                     GRAVFLVDTYDTANGVRTATRVIRDLGLTDRLGIRLDSGDLEQLSREARRILDDEGLP
                     HVRIFASGGLDEHDVDRLVRAGAPIDAVGVGTRVGVSADAPYLDSVYKLVEYDGRPVL
                     KLSEAKATEPGRKQVYRTAQRDLVALRDEPPPPGAEPLLVPVMLEGHRTGPPRNVETA
                     RRLFESDLVRLPGGATLIDRPEAPVAGFSESLERLTEESRARALRSAGLPG"
     gene            15684..16787
                     /locus_tag="VE646_03685"
     CDS             15684..16787
                     /locus_tag="VE646_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZD79127.1"
                     /translation="MRFLERSDFDRLLSSLVESGYDLIGPTVRDGAVVLEPIDGVHDL
                     PRGVRDEQGPGRYRLSPTGGDELFAWSHGPDSAKRFLFPAREVLGTVARGDSRMSPAT
                     SQDGERPLAFVGLRSCDLHAVAIQDRVFMEADPAYRRRRERAVFIGVNCEHPGSTCFC
                     ASMGAGPRCAVGFDLALTELPTGFVVEVGGPRGEELIEAVTTREASEEEEDAALRVTR
                     EAEVRMDRTLDTQDMPEVLYRNRRHPRWKEVAERCLACGNCTSACPTCFCHDLVDASD
                     ITGARATRTREWASCFSEEYSWAAGASVRAGRDARYRQWLTHKFASWIDQFGTSGCVG
                     CGRCITWCPVGIDVTEEVAVIRATDGEVEVVAQ"
     gene            16784..>17167
                     /locus_tag="VE646_03690"
     CDS             16784..>17167
                     /locus_tag="VE646_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit gamma"
                     /protein_id="HZD79128.1"
                     /translation="MSEAAKLLEPEGTLGRVGVIRRVVPETPDATTFWTTFRAGDGEG
                     YAFRPGQFNMLYLFGIGEVPISISSDPGHPESLGHTIRFVGRVTNAFRDLAPGDEIGI
                     RGPYGRPWPLEEAEGGDVVVVGGGVG"
BASE COUNT         2561 a         5843 c         6073 g         2690 t
ORIGIN      
        1 cctgcgaccc cggagggtca gagcgggctt gatgctgaac agccggacca cgacctcgct
       61 cctttctcta aaactaagac ctctaggtgt tagggcgaac cctacggaaa gatggagctt
      121 ctgtcaatcc tcttgccggt accccagatg aggtgcttgg atccccctcg cctgttctcc
      181 gtatcgctag gatctcttgg tgtaggattg atccatgctg gagcggctga ggcgggacgc
      241 gcgggcgctt ggggcgctgg aggacgaact ccgtcgctcc ctgtacctgt tcgtacgggc
      301 cggcggacgg ccggtcaatc gggaggaggc ggcggcggct gtcgggatct cccgcaagct
      361 ggccgcgttc catctggaca agttggtgga ccgaggcctc ctcaccttca cctacgaacg
      421 accgcccggg cgcggaggcc gcggcgccgg gcgatcggcc aagtactacc gaccctccga
      481 tcgggaggtc gaggtgtcca tccccgagcg gcgctacgac ctgctcgggt cggtgctgct
      541 gcgggccctc cgatcgcggg aacccggtga ggccgccggg gaggccgcag gcagggcggc
      601 ccgtgagacg gggttccaga tgggacgtga cgagcggcag ggtctcggca tccctccggc
      661 cggcccggaa cgaacgatgg ctctggccgt tcgactgctg gaacggacgg ggttcgagcc
      721 ctacgaggac gagccgggct ccatccggct gcggagctgt ccgttccagg cgctggccga
      781 gcgggatcgc gatctggtgt gcggtatgaa ccactgcctg atcggcggcg tggtcgcggg
      841 gctggggagc gaggccgtcg atgtgctcct tgatccgctg ccgggtggat gctgcgtcac
      901 gctgaaggcg ccggcgcact agggctcagc cgctcgaggg ctccagcgcg ccgaccatcc
      961 ccaagctctg gtggtacttg cagaagaaga ccgcttggcc cgactgcggg atggtcacgg
     1021 tgaccgtctg gtgctcgccc ggcgacacgt cctggtcgat gctctggccc tggatcgaga
     1081 agttgtgcag cacgccgctc tcgttcttca gctcgatggt gacctgctcg ccaggcgtgc
     1141 ccttcacgat ggtggggtcg aagtagaagt cgttcatctg cacctcgagc tggccctgtg
     1201 cgctcacgtc cttcgtgccg tgatcgttgg cccgctggcc gccgatcgtg atcgtgccgc
     1261 ccccgcctcc gccggtggcc gtcgggctca gggttgggct cgtggcgggc tcaccggtgg
     1321 ggctggccgt gcccgatgca gcggtgcccg agccacccga gcaggccgca cccagcaacc
     1381 ccagggccac cgtcagcgcg atcagcgacg tccgcttcct catctcctca ctcctcatcc
     1441 cgtcggacca gatgccggtc cttccatgcc cgctgcggac cggatctctg tcacgtgccg
     1501 gccaacccac accaggcggc gtcgtgccgc ccggtggtct acggacgccc gggctggatg
     1561 gttcatcagc gggcctgatc tcccgatggt tccaccgggg cgatctccgc ttcggtcaag
     1621 gccgaccgcg cgaggagttt tgccccttcg aggaccgccg gtgcagggcc gggtcgggcc
     1681 ggggggcctt cagcgatccg gcccggcctt gccggcgctc cgctggaggt ctcgggcctc
     1741 gcctcggttc tcgtgggcct cgttgagcgc cgcgatcgac accagcaggg ccgcggccgg
     1801 gtcggcccac caccacccca gcccggcgtt cagggccagg ccggcgagcg tcgccgtcga
     1861 cagcatcccg tcgaggaagg tcatcgaggc ctcggaggcc agcggcgcgg agccgagccc
     1921 ggcggccgtg cgtcgcttgg ccacggccag cccgaacatc accagggccg tcaccgccag
     1981 gtacgtgatc ccggcgagcg agtgcccggc ccgctggccg cccgcgaggt tgcggatcga
     2041 agccgcagcc aggatcaggg ccagagccag gaaggccccg gccaccaggt tcagcgccag
     2101 cgccgtccgg tcgggacgat cgatctggtg accgggccgg acgtgccaga ccaccacgcc
     2161 ggaggcgaac acctcgatga cggagtcggt gccgaagccg acgagcgcca gcgagccggc
     2221 cgccagcccc agccagatcg tcagcacggc ctcgcccagg ttccacgcga tcgtcgcgta
     2281 ctcgagccgc aaggctcgcc tccgcaacgc gtccctggcg gtcgtcgtcg ccatgcccac
     2341 atcatcccaa ggcgaacatg ccggccaacc cgaacgctct ggtgcccgtt gggttgacat
     2401 tcaagtcgac ttcaatgcct agcctcggtc catcgacggc gatcggcgac gacggtggat
     2461 gccagatgga aggagcgtca tgggtctgaa cgaggtggag atcgagtact gcgtgccgtg
     2521 cggtctgttg cccacggccg aggagacggc gcacacgctg ctgtccacct acgggcagcg
     2581 tctgggtggg ctccgtctga agcccgggca cggcggggtg ttccgggtca gcgtggacgg
     2641 cacccgcgtc ttcgacaaga gcgaggacgg ctacgacgtg gcggccatcg tgtcccgggt
     2701 ggacgagcgc ctggctcccg agcggagcct tcgttcctga gcgaagggca ggctcgggcg
     2761 atggtgcggt ggacggtaaa gtggacttga atcttcacca aggggcaggc tatgtcgacc
     2821 tacacgatct ccgaggtggc gacccgcacg ggcttctcga ccccgacgct tcgctactac
     2881 gagcagatcg gactgatccc gcggccggag cgtaccgagg tcggctaccg ggtcttcacc
     2941 gaagagcatc tccggatcct cgacttcatc ggacgggcca agcggctcgg cctgccgctg
     3001 gaggagatcg cggttctggc cgaagcctgg gccaagcggg attgccgcgc gacccgggag
     3061 caactcgagc gcctggtcga gaccaagctg acccaggtcc ggcacctgat cggcgacctc
     3121 atccggtttc gggatcagct ggaggaggtc cacctggagc tcacggatcg accggcgccg
     3181 cgccggtgcg gacctgactg tggctgcgag atcgaggtca acacgatcga tcgggacgag
     3241 gtccgcgtgc tctcgctggt ctcgctcagc ccgacgcacg gctgatctcg ctgaccatgg
     3301 tgcgtgagcg atctgcggtc gggtagaaca gcgtctcggc gagtcgacga gcgcccgtgg
     3361 ctcagcggtg agaggatccg acgggcaccg agcgctctgc ggcaggtagc tcaggtggta
     3421 cttgaaggca gcggatccgg cgatgacctt cccccggacc tcgaacgccg gcataccgta
     3481 ggggatgcct tggtcggcat cgggaacgat cgccaggatg gcctcgcgga ggtcggccag
     3541 ggctccgttt gggctcctcg agtgcttcga ggtactcgtc gatctcctcg gccgacacgc
     3601 tccatcctat ccaggcctgc tggtccaggc ctgctggaac ctcgcctgag acggcggtcc
     3661 tcgggatcct gcttgaccaa cgccccggcc cagctcagag gatccggcgt agcggtgtcc
     3721 gcgtcacgag cggccaagcg agggcgaacg agcccacgat cccgagtgtg gccacaagaa
     3781 gcgccttgat gtctccgggg atagcggcgg gtcggagcgt tagctcaagg gcgatgatca
     3841 ccgggccctg cagcatgaac gccgcgtagg agccgcgggc catgatctgc cgcagcggcc
     3901 ccgtcgcgtt cagatgccgc tggacgaagg acaggaccca gatcggtccc gagacggcga
     3961 ggatgccctc gctcatcgcc cacaccagcg ccaacccacc ccagccaccg aagtacacgt
     4021 cttgacccag gccgcgcaaa tccgcggtca cggcaacggc cacgaccacg agcgacgcag
     4081 cgatggtggc gatcccgctg catcgcgaga cagcgcgcgg tacgtcgcgg agccatcctc
     4141 gtccggccgc cacgatcccc aggccgaacg tcgccaggta ctccggccac ccccacgcct
     4201 tcacgtttag cggctggttg gagtccatcg ggagggccag ccgcaccagg aacagggaga
     4261 tgccgacgcc gagcgccagc aggaccagcg tcggcccgcc cagcgctcgc tccgaaggtc
     4321 ccccttccgg gcaccgccag cgccatgcga cgtagaggat cgagtagagc aggagaaccc
     4381 ccacgaacca caccgggccg ttgtccagta ccgggtcggt gttcgtgacc gagttccaga
     4441 atcctcggtg acggaacggt ccgagcagcg cgtactccag cagcggccac accagcagcg
     4501 tgtagaccgc cagcggcaca cccagccgca gaagacgatc gagggtgaag cgggtcggtc
     4561 ccttgcgttg gagcgatccc ggggtcagca ggcccgagac caggaagaac agggccatga
     4621 ggaacagtcc accgaggagc aggtacagga gggcgtacac cttctctacg agctccgaca
     4681 gcgtcgtctc ctgcacgtcc tggtaggtcc aactgccgaa ggtcgcgtac cccatcacgg
     4741 catgcgaggc gatgatgccc gcgatgagga ccgtcttgag gttgtccagg tatgccagcc
     4801 gcgttcccac cggggcccga cggacgaggc cctcgcccag ctgtgtgggc cccatcggtg
     4861 gggggatcga gatggctccc cgctcggcca cgcccggccg ccgaaccgtc atgcgcccct
     4921 cccggccgcc gtccggccga tgatccttac ccccagtccc tccaaccgcg accgagggcc
     4981 ggccgacgcc caggggatca cccacggatg gggggagctg gccgtgcaac ctcgcgatgc
     5041 gccgatcagg ggggcggtga agttcttcat gatcgaggcc aggccgtggt acggccatcc
     5101 ttcggtgggt cagccgagcg ggggacgagg agagctctca gaaggaccgg gggcctggga
     5161 tgcacaggcc cccgagcacg gcacgagcgc caaacccgga gcaaggcgct cgcgggagat
     5221 tctggaggat gcgtgtacag gccgggtcct tcggcagagc acccgcgagg ctcgccgccc
     5281 atcgcgaccc ctccctcccc ggcgtggcac agagctggga tcctgcctcc tcggcgggtc
     5341 tcccggtcca ccgggtcacg cgggtccacc atccctcgcg cgcctcagcg tgtcgcgggc
     5401 ctggtgaccc gggacgttgg ggaccgactc gcaccctcgg gatctccacg gtcggtgcgc
     5461 ggacgaagac ccgggtccgg tgacacactt gccgctatgg aggagctttc cgcccggggg
     5521 tttttcgatg cactcgccgg cgagtaccac ctcctgctcg atgactggtg ggaggcggcc
     5581 gagacccacg gtcggatcat cggcgggctc ctgcgtgagc acggtgtccc tcccggggag
     5641 gggctgctcg actgcacgtg cggcatcggg acccaggcgt tgccactggc tcgccagggg
     5701 tatcgggtgc tgggcacgga cctcagcgcg gcggcgatcg cgcgtgcccg gaaggaagcc
     5761 aaggagcgag gcatccaagt cgagctcgcc gttagcgatg tccggcaggt tcatcggctc
     5821 gtcccctccc ccgttgaggc cgtgatcgcg tgcgacaact cgttcgccca cctgctcacc
     5881 aaggacgacc tgcgggccgc tctccgctcc gtccgggcct gtctggcccc aggccggctg
     5941 ttcctggcga gcatccgcga ctacgacagg ctctcggagg agcggcccac gggcgtcgtc
     6001 cctctcacgt accccaccgg cggtccgcgc aggatcgtgg ggcaggcctg ggagtggtcc
     6061 gagtccgccg aggcgctcct gatcaggctg ttcatcttga gggaggagca cgatgactgg
     6121 tcgtgctcct tgtgggccac ccggctccga gcctggcgcc gccacgaggt cgactcggcg
     6181 ctcggggtgg ctgggttcca cgacatcgtc tggcacttcc ccgatgacag cggctactac
     6241 cagcccatcg tcaccgccag ggcgatgtag aaggccgcta ccctggcgag tgcccctcag
     6301 cctagaggtc tcaggccgac atggatcgca gaacctcccg cgcccgagcg gcgcggacga
     6361 ggttgccctt gcgctcccac atctccacgg cctgcgtcgc cagatcgatc gcctcggcct
     6421 gccggccggc ctcgttgaag atcggaccga ggtccatcca aaccccagcc tgcaggtcgg
     6481 tgtcctggat cccgtgcgtc aactccacgg cctcgcgccc ggtcctgacc gccgactcca
     6541 ggtccccccg tgcggccagg atcttcgccc gcgcccggcg gagcaggatc tggtccatga
     6601 cgtcgctgtc ggcgctcatc gaccaggccc gggcggcctg ggactcggcc tcctcgaacc
     6661 ggcccatatc ggcgagcacg tcggcgagca aggcgaggaa ccccgacagg tggcccagat
     6721 cgcccatctc ctcgaacacc gagcaggcgc ggcgcactgc cgcctccacc gccgggagcg
     6781 atgcggctca tcgaggacca ctccgaacgc gtcgggtacc tgctgaagga gcgtgtgttc
     6841 gacgtcgcga tcctggtgga cgccctgcgt cggatcgccg acgccgaatg cgtgatcgat
     6901 ccgacgatcg tgtcccggct cgtcggccgg cgccggcggg cagacgcgct cgaggccctc
     6961 acgccgcgtg agcgagaggt catcgggctg gtggcggcgg ggctgtccaa ctccgcgatc
     7021 gctgaacacc tctacgtcac cgagcggacc gtcgaggcac acaccaagca gatcttccag
     7081 aagctcgctc tcgaggcaga tccaggctcg aaccggcgcg tgctggccgt tctcgcctac
     7141 ctccgttctt aggtccgtga taggggagca gcgtattgca gctccatggc tcggcgggat
     7201 gcctcctcga catcatcggg gggttcggag gagaatcctg caccaccgag tggcggagga
     7261 ggacggtcac gtgcatgggc tagtggtgag ggtaaacatc gagtcgggtc gggaagccga
     7321 ggctgcggag ttcctgaagg ccaccgtgct tcccgccgtg aagcagtcac cgggcctcgt
     7381 cgctgcatac tggtggcagg gctcggaggg agggcagggg tcgtcactcg cggtcttcga
     7441 cagcgaggag gcggcacgag gggctgcagc catggcctcg agcggtcagg tccctatgcc
     7501 cgactacgtc tccatggcgg gcgcggacgt cgtggagatc caggcgtcga tctgacgcgc
     7561 tcccgaagcg ctcgctctta cgactcgagg tctacgtcgg cccctaccgt cccgcggctg
     7621 ctcctgctgg tccccgacgg acagggctcc ggaacgatgc ctgcgggagt cgcgagttgg
     7681 actggagtcc gtgctcagga gttcaccaga cgcaggttgg gaccgctcgc tacacttctt
     7741 tgtgtgcttg cggtcgcttc gtgacccgcc ttgtatgacg ctggggagcg gtgagcggca
     7801 ggatggggga gcgtcgctgc aggtagacgg gcgccggtag ctcaacggat agagcatctg
     7861 actacggatc agaaggttgg gggttcgaat ccctcccggc gcgccgatcg cggttcagga
     7921 cgtcgggaac cttccctcca agaaccccgg gtcgcggtag gcgggctcgg ggtagcggta
     7981 gaagccctca ccgctcttcc tacccaggcg ccccgtgtcc acgtatccgc gcaggtactt
     8041 ctcgttcttc tccagctgtg gatcgccgag ctgctgagcc cagaaggacg tgatcttccg
     8101 gacggtgcca cggcccacgt cgtcgagcat cccgaagggg ccgacgggca tcttcgtgac
     8161 tcccatccag gcccggtcga cgtcttccac gagggcgatc cggttcgcgg cgagcgcgat
     8221 cgcctctcgg ttcatggcct cgtacatcga gttgaaggcg tacccgtgat gctccttgcg
     8281 cagatggatc gggatctgcc cgatccgagg cgcgaaggcg agcagcagct cggttacctc
     8341 gggggcggtg cctggatggg gcatgacgtc gacgacgttc gccgaccaca ccggcaagtg
     8401 gaagtgcagc gccgcgaacc gatccgggcg accggtcgcg tccgcgaaca tcgaggggat
     8461 caggctcgac gtgttcgtag tgaacaccgt tcgttctggg cacgggcgat cgaaccgcgc
     8521 gaagaccgcc ctcttgagct ccgggtcctc gggcgccgac tcgctcatca ggtcggcctc
     8581 ttcggcctcg gcctcatgat ccgggcggtg ccgatcaggg gcagcgcccg gtcgcgctcc
     8641 gacccgtcca tcagacgggc cgccacgatc tggtccgcgg tagagcggat gccttgcatc
     8701 ctggcccgga gggccgacgg ctgcacgtca tggaggacga cctcgtaccc gtgcatcgcg
     8761 cactggaggc cgatctgctg ccccatggtg ccggcaccga cgatcaacac aggtcgcacg
     8821 tcatcgatgt tcatccttca ccttccaaga gctgccgcga cgactccatc cggtcgatct
     8881 ggcggagctg ggagtgacgg atgttccggg ccaggagccg gaggcggagg tcgcccggct
     8941 cggaaagctt ccgctcgctc atccggatcg cacccaggtg atggggaacc atgagttcca
     9001 ggaagcggcg atcgaaggcc cggccctgca tctccgccag gcggtcgacg tccccctccg
     9061 gaggcatccc cggcatctcg tcacgctcct ggccgctcag cggctcgacc tcgcctccga
     9121 accacgccgc ccaccagtcc gccatcagcc ggatctccgc cccgtgctcg gccatcaatc
     9181 ggcccagagc gcgaagatcg gggacctcgg cccgctcggc ggccagttcg gccagccgat
     9241 ctccggtccg gtggtgcccg gtcatcatgt gcaggaaccg gcggtcctcc tcctaacccc
     9301 ggatcgccca ccggagccca cgccgggttc gtgaccggac gtcgatgaag gccatcatgg
     9361 ggatcgacgc gcccaggagc gctgccgccc tgcgccccac cgtccgatcc ccctcgccga
     9421 acagcatgga cagcggatag gcggccgccg agctgaggtg cacgcccata gcggtccagt
     9481 acggcacctg catccgtagg atcggctgca cccaaggcag cgccaggtag tactcgatgg
     9541 ccgcggtggc ggcggcccaa agcgccgccg tggccagcag gaacgccgtc cggcggcgac
     9601 gagggacccg ggggacgatg gtcccgaaga agaccacggc ccagaagaaa tcggcgagct
     9661 ggtgcgcgag gatcccagcc gagacgccga ccggacccgg gcgggaacgg atcgctcggt
     9721 tcctgagcgg gacggtgcca acctgcatcc agtcaacgag ggggtcgcgg cccgtatccc
     9781 gggctaggaa ctgcacgaac agcgtggagt atgtggagga caggagaccg gtcagcgccc
     9841 ccgcccgcag atcgctccac gcgtgctcgt cccggcgcct caggaactcc ctcacggatc
     9901 gcatcccacc tctgttcctg cccaacgtga ggaggaccca tgccaccgat cccgcggtat
     9961 ggaaggccac gaggatggta ggcggccgat gatggcctcc ttcgacgagt tccgggtgcg
    10021 gaccgacgac ggtgtctccc tgcatgctcg ccgatggagg gcgtcgcggg gcggcctgag
    10081 gacacccctc cgaccacccg cccgagcgat gcttccgcta cgggctcgcg ggaccagcgg
    10141 gtccgaagac ccgtagcggg ggggacccgc cgacggaccc cggccgccgg gacgacgcta
    10201 gcctgagggc gtttcctgat ggccgcggaa gaccgggctc gggacgccct cgagatgctc
    10261 gatgaggagg agcgccaggt gctcgtccga gtcgcaggca tcctgcgccg gatcgacttc
    10321 gggacggtcc tgctggtggt gcaggacggc aaggtcgtcc agatcgagat ggccgagaag
    10381 ttccggttgc ggtgatcgcg tcgggacctt cggcccatgg caccgctccg gtgccgaagt
    10441 agcgttcgct gcaacatcgg ctgaccagac gactggaggc cgcgacgcat cggcgtcgcg
    10501 gcccttcttc gtatgagggg aggttgggct catgaggaag gctccggccc tcctgttggt
    10561 ggtggccctg cttaccaccg gatgcgcgag ctcggcatcc agggccgagg gaagctcgga
    10621 cgtgctacgg ctggggatgt tccccaacct gacgcacgcc cccgcgtacg tggccctggg
    10681 ggaaggcatc ttcgatcggg acctggcccc gaccagggtc gaggtcacgt acttcaactc
    10741 gggcagcgat gctcaagccg ccgtcaccac cggggcgatc gatgcgacct acatcggccc
    10801 gggaccctcg atcaccgcct acctgcagtc cggcgaggtg gccatcgtgt ccggtgtggc
    10861 ctccggtggt gcctccttcg tcatccgcaa gggatcgggg atctccgagt cggcggatct
    10921 gcacggcaag aaggtcgccg tccccgggat cgggaacacg caagacgtgg ccctgcggac
    10981 ctggctcaag gaccacggcc tccgggccaa ggacgcgggg ggcgacgtgg cggtggccgc
    11041 gatcgacaac cccgagctcc tgcagacgtt ccaggcaggg cacgtggacg cggcctggga
    11101 gccggagccg tggccgagct tcttgatcca gcagggcgtg gcgcggctct tcgtggatga
    11161 ggcgacccta tggccacggg gacgcttcgt ggtcgccaac ctgctggtga acacgatctt
    11221 cctgaaggcc catccccaca tcgtgaagcg gttggtcagg gccaacgtcg aggcgatcca
    11281 agccatctcg gacgacccca cccacgccca ggaggctgcc caggccggac tgatccaggc
    11341 cggcgctccc tccttcgacc agtccgtcgt ggacgcggcc tggtcgaagc tcgagttcac
    11401 ctgggatccg ctgcccgaga cgctctgcaa ggacgctcgg gatgcccacg ccctcgggta
    11461 cctggagcag gatccgaagg acatctcgcg catctaccgg ctgggcatcc tgaacgaggt
    11521 gctgaaggaa gagggggaac ctccggtgga ggttcccgca tgaggacctt gccacggctc
    11581 cgtgcggtgc cggacctcga gacggcttcc ccgaagctgt cggcgcgcgg cgtgtcgaag
    11641 tcgttccgta gcgcccgtgg cgggacggtg ccggccctgg tcgacatcga cctcgagatc
    11701 cgtcggggcg agttcgtgtg cctgatcggt ccctccggat gcggcaagac gaccctgctc
    11761 aacctgttcg ccggcctgga tgccgcggac cagggggatc tccggatcga tggtgctgcc
    11821 atcaccaggc ccgggcccga tcgcgccgtc ctgttccagg agccggccct gttcccttgg
    11881 ctgagcgtcc tggccaacgt ggagttcgcg ttggagatgc tgggggtgcc gaaggacgag
    11941 cggcgggacc gggccatgca ctggctccac cgggtccatc tggggcggtt cgccgatgcc
    12001 cagcctcacg agctctccgg tggaatgcgc cagcgggccg cgatcgcccg ggccctgtcg
    12061 tgtcagcccg aggtgctgct ggccgacgag cccttcgccg ccctcgatgc ccagacgcgg
    12121 gagatcctcc agggcgagct ccaggaggtg tggtcggaga cgcggaacac attcctgttc
    12181 gtgacccaca acgttcgcga ggcggtcttc ctggccgacc gggtgatcct gatgtcggcc
    12241 ccgccgggaa ccttggtgac cgagcatcgg atcgaggcgc ccaggcctcg cgacttcgag
    12301 gacatcctgc tgtccaaggt ggtggtggac atccacgccc acctgtcgag ggaggtggag
    12361 aaggttgtcg cgcgtgaggt cggtccttag ccagctctgg ccgcggctcg cggcgatcgg
    12421 cggcttgctc ctggtgtggt ggctggcgta ccggagcggg ctcttcaacc cggcggagct
    12481 tccgtcaccc ggccaggtga tcaccgcgtt cggcgacacg ttcttcaagc cgcacccccc
    12541 tcgggaatcg atcctggcgg ccacccaggc cagtctcatc cgcctgtgcg tgggtctggc
    12601 catgggaatc acggtgggaa cgtcgatcgg cctcagcatg gccgtctcgt ccagcgtgca
    12661 gcggagcgtg ggcagcctga tgtcggggtt gcaggcgctg ccgtcgatct cctggctgcc
    12721 cctggccatc ctgtggttcg ggctgaccga gcgggcgatc ctgttcgtgg tgatcatcgc
    12781 ctcgatccct gcggtggcga tcgccgccgc gtccagcatc cggttggtgc ctccgctgtt
    12841 cgtgcgggcc ggtcgcacga tgggcgcgcg cgggtggacg ttgtaccgac gcgtggtgct
    12901 gccggccgcg gtccccggat acgtgggcgg gctgcaatcc gcgtgggccc tggggtggcg
    12961 tgccctgatg gccggggagc tcatctccac tgggaacaag ggcctggggc atctgctgga
    13021 tgcgaaccgg cagctgttgc tgacctcgaa catcttcgcg gtgatgctga tgatcatcgt
    13081 ggtgggcatg gcggtcgaga tgacgctggg atcgatcgac cggcgggtcc gagctcggcg
    13141 gggtctcgtg ctcgccacct gatcgacatc cggccgcgga cgatggctcg cccccgggcg
    13201 ctgacgcagg ataggctccg gtcgatcgga ggggaggacg tgccgtgcag aggaaccgct
    13261 ccatcctgtg ggcgctgctg ctgatcgcga tcggcgtggt cctgctcctc cgcaacagcg
    13321 gcacgatcgc tgaggacgtt cggatctggc ccatcctgct gatggcggtc ggcgcgtggc
    13381 tgctgatcga gcgcctggcg ttcgggacgc ggtggggcgg gggttcggcg cgcccctggt
    13441 gatcctggcg atcggggtca cgttcttcct gcaggacgtc ggggcgatct ccacggacat
    13501 caccatctgg ccggtcgtcc tgatcgcggt cggcgtcgcg atcgtcctgt cggcggtccc
    13561 agcccgtggc cgccgcgctt ggggaccggt gagggagacg gtgccgctgg acggtgccgc
    13621 ttcggcccgg gtggtggtga agcacggagc cgggcggctg accgtgacct cacaggtgaa
    13681 cccagacctt ctggtcgatg gctccttcgg ggccggggtc cgcaagcgcg tgcggcggag
    13741 cggcgatcgg ctggacgtga tgctgtccca ccagggctgg tggatggagc acgcgaggtc
    13801 ctggagcggg agcgggggac ccctcgactg ggaggtcggt ctctcccgac tcgtgcccct
    13861 ggagctggag atggagggtg gggcccaccg ggcccggctg gacctgtcgg acctgcaggt
    13921 tcgcgagctc cgggtggaga ccggtgccag cgagacgacc atcacggtcc cctcgaaggg
    13981 ccactgccgg gcccgggttg aggccggtgc cgcctcggtc acggtccgga tcccgccgca
    14041 cacggcggcc cagatcaggg tgcagggtgg cctgtcgggc gtccgtgtgc ccgagacgcg
    14101 cttcccggcc gtccccggcg gctaccgatc gccggacttc gaggaggccg cggaccgcgt
    14161 ggagctcgag atccaggcgg gagccgcggg gatcacgatc gagtgacggc gcgccattca
    14221 gccgggcagg ccggcgcttc ggagcgcacg ggcccgtgac tcctcggtga ggcgctccag
    14281 ggactcggag aaccctgcca ccggcgcctc gggccgatcg atgagcgtcg cccctccggg
    14341 cagccggacc aggtcggatt cgaagagccg ccgggccgtc tcgacgtttc gcggcggtcc
    14401 cgtgcggtgg ccctcgagca tcaccgggac cagcagcggc tccgcgccgg gcggcggggg
    14461 ctcgtcgcgg agggccacca ggtctcgctg ggccgtgcgg tacacctgct tgcgcccggg
    14521 ctcggtggcc ttggcctccg agagcttcag caccggccga ccgtcgtact ccacgagctt
    14581 gtagaccgag tccaggtagg gcgcgtccgc ggataccccc acgcgggtcc ccaccccgac
    14641 ggcgtcgatg ggggcgcccg cccgcaccag gcggtccacg tcgtgctcgt cgagccctcc
    14701 gctggcgaag atgcgtacgt ggggcagtcc ctcgtcgtcc aggatgcggc gggcctcgcg
    14761 tgagagctgc tccaggtccc cgctgtccag ccggatcccc aggcggtcgg tgaggccaag
    14821 gtcccggatc acccgggtgg ccgtgcggac gccgttggcg gtgtcgtagg tgtcaaccag
    14881 gaacacggcc cggccgggga agtcctgcgc gaacgccctg aaggcctccg cctcggtcgg
    14941 gaaggcctcg atgtaggagt gggccatcgt gcccgtcgcc gtgagcccgt agcgccgagc
    15001 ggcctccacg ttgctggtcg ctgtgaagcc ggcgatcgcc gtcgtcctgg ccaccgccat
    15061 cgccgcgtcg gtgccttgcg tgcgccggaa ggcgaagtcc accacgtccc ggtcgcgggc
    15121 ggccagtcgg cagcgggcgg ccttggaggc gatcgccgtc tggaacgaga tctggttcag
    15181 caggaaggtc tcgaccagct gcgccaccgc gataggtgcc gtgatctcca gcagcggctc
    15241 gtcggcgagg aggacccgtc cctccgggac ggcccacacc tcgccgtcga aacgggtctc
    15301 gcggaaggcc tccaggctgg gctcgtcgaa gccgagctcc tcccgcaggt atcggagatc
    15361 ctcctcctcg aacccgaagc cctccaggaa ctccaggcac ggctccagcc cggcggcgac
    15421 caggaatccc cgctccgggg ggagccgccg gacgaacagg ctgaaggtgg cggtccggtt
    15481 catgccccgg cggaggtagc tcgccgccat gttcagctcg tacaggtcag tgaacaggcc
    15541 cccacccatg gcgtacaggc tacgcgaagc ccggtgccac cccgacccta ggtcgtgggg
    15601 gcaaggggcc cgggaccggt gcgcccttcg tcccctggca catcgctatc gctcggggac
    15661 gctatccgtg gaggtgtgag cccgtgaggt tcctcgagcg cagcgacttc gaccggctcc
    15721 tgtcgtccct ggtggagtcc ggctacgacc tgatcggacc gaccgtccgc gacggggccg
    15781 tcgtgctgga gcccatcgat ggtgtgcacg atctaccccg cggtgtccgc gatgagcagg
    15841 gcccgggccg gtaccggctg tcccccaccg gcggggacga gctgttcgcc tggtcccacg
    15901 gaccggactc agccaagcgg ttcctgttcc cggcccgcga ggtcctcggc acggtggcca
    15961 ggggggacag ccggatgagc ccggccacgt cgcaggacgg cgagcggccc ctggccttcg
    16021 ttgggctccg ttcctgcgat ctgcacgcgg tggcgatcca ggaccgcgtg ttcatggaag
    16081 ccgatcccgc gtaccgccgg cggcgggagc gggcggtgtt catcggcgtg aactgcgagc
    16141 accccggctc aacgtgcttc tgtgcctcga tgggtgcggg tcctcgatgc gcggtgggct
    16201 tcgacctcgc cctcaccgag ctacccacgg gttttgtggt ggaggtcggt ggtccgcgcg
    16261 gggaggagct tatcgaggcg gtcaccaccc gggaggcctc cgaggaggag gaggacgccg
    16321 cgctccgggt gacccgggag gccgaggtcc gcatggaccg gaccctggac acgcaggaca
    16381 tgccggaggt cctgtaccgg aaccggcgcc accccaggtg gaaggaggtg gctgagcggt
    16441 gcctggcatg cggcaactgc acctcggcct gccccacgtg cttctgccac gacctggtgg
    16501 acgcctcgga tatcaccggc gcccgtgcca cccggacccg cgagtgggcg agctgcttca
    16561 gcgaggagta cagctgggcg gccggcgcct cggtccgggc gggacgggac gcgcgctacc
    16621 ggcagtggct cacccacaag ttcgcttcct ggatcgacca gttcgggacg tccggatgcg
    16681 tgggctgcgg gcgctgcatc acctggtgcc cggtgggcat cgacgtgacg gaagaggtcg
    16741 cggtgatccg cgcgaccgac ggcgaggtgg aggtggttgc ccagtgagcg aggcggcgaa
    16801 gctgctggag ccggagggca cgcttggtcg ggtaggggtg atccgccgtg tcgtgcccga
    16861 gaccccggac gcgaccacct tctggaccac cttccgggcc ggggacggcg agggctacgc
    16921 gttccgcccc ggtcagttca acatgctcta cctgttcggg atcggcgagg tccccatctc
    16981 gatctcctcg gaccccggcc accccgagtc cctggggcac acgatccggt tcgtgggtcg
    17041 ggtgacgaac gcgttcaggg acctggctcc gggcgacgag atcgggatcc gcggcccata
    17101 cgggcgtccg tggccgctgg aggaggcgga gggcggcgat gtcgtcgtgg tcggcggcgg
    17161 ggtcggc
//
LOCUS       DASQQQ010000088         5474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_33594, whole genome shotgun sequence.
ACCESSION   DASQQQ010000088 DASQQQ010000000
VERSION     DASQQQ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33594"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VE646_03695"
     CDS             complement(<1..418)
                     /locus_tag="VE646_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79129.1"
                     /translation="MDPIQYLRAFRRRWAVIVAAVLVAAAAGWLTTSTVAPVRAAPQR
                     YSATALLGNGTSSTVPGVPILDVPALVRVATLPDVVDLAAKELGWTGDPSVLAGNVQV
                     TPDPQSDGFLDITGTATGPRQAERIAEAFSNALIAFV"
     gene            complement(397..951)
                     /locus_tag="VE646_03700"
     CDS             complement(397..951)
                     /locus_tag="VE646_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZD79130.1"
                     /translation="MSSRTVLVAHPEALTAEALAAALARFPGLVPVGVTTDADELVAM
                     GVRAQAAAVDGRLPGARDAAARLRRLGVRVVLIEQPTDGAEDEGIRVPLHAPVSRLAA
                     ALAPESGAVDGSRGLTRRERQVLTLVAKGMAGKQVARLLGISPKTVEQHKTRIFEKLG
                     VHNQAAAVAVVAARGSAAWIPSSI"
     gene            complement(948..2831)
                     /locus_tag="VE646_03705"
     CDS             complement(948..2831)
                     /locus_tag="VE646_03705"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HZD79131.1"
                     /translation="MDRAELRQTVPGRLVVLFRHDVPLILLDLLAVFAAYLAALLVRL
                     NFDVPADMWAGFRSFFPALMAAHLLANYLFGLYGQMWRYASVQEARRLGLAGITAAVL
                     VMGADQWLGVPMPRSAALMGAAGALVAFGAIRFQSRLFGFRRREVAMDEGRVKRVLVM
                     GAGDSGAQLLSDVLRHPEVGLRPVGMVDDDPRRQRLALHGVRVLGTRDDIPSLAHRYA
                     VDEVLLAVPSATSEVIRDVARRSEEAGVSLRVLPSVRQLVGGRVGVRDLRDLHIEDLL
                     GRRQVETDLEAVGGLLRGKRVLITGAGGSIGCEIALQVAGFEPDRLLLLDGDETHLHD
                     LLVSLGASAKAVPVLADVRDRTRMVELFLEHQPQVVFHAAAHKHVPLLEDHPQEALAT
                     NVLGTANVADAAVTSGAERFVLISTDKAIRPSSVMGASKRLAEDVVRSLSGRGTVLCA
                     VRFGNVLGSRGSVIPTFLRQIQAGGPVTVTDPAMTRYFMSVQEAVQLVLQAAALSRGG
                     EVFTLEMGEPVNILDLARRLMHLSGRVPERDVRIEICGIRPGEKLHEELVDADERPMS
                     TDHPGIVAAASPVPDRALLRARLSELEGLAGEGRLAELAELMRLPVGPTKAEAEPVEA
                     RLP"
     gene            complement(2838..3689)
                     /locus_tag="VE646_03710"
     CDS             complement(2838..3689)
                     /locus_tag="VE646_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79132.1"
                     /translation="MSKLDRLGWVVCRWFQLGETRFGVRTTSPAFGGWLDHVLGAYAV
                     DGPEDPDAEPYYGLVVEDGSGASGRVGRRFHILYEGTWDVVRTMDLRTLARAFLLEVE
                     ALTFPGRDDVIYLEAAVVAGGGRTAMVPSYMVPALARAGRRVQRAAHAVLPGTLAVAI
                     DPDTGKLIPVPRRLEVPDDALEILQAPPEGPDPRAWVEVPVPADRVMLFGPEPGLEPL
                     PRAEALVQLASRTKNLPVLGGRAVRGLARLLETAQPCTTRLMGTEHMIRTVLALLDGG
                     PGTGNGG"
     gene            complement(3764..4063)
                     /locus_tag="VE646_03715"
     CDS             complement(3764..4063)
                     /locus_tag="VE646_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr-rel-A system PqqD family peptide chaperone"
                     /protein_id="HZD79133.1"
                     /translation="MSRPMMRSDLTVVELDGEAVIYDEETGDLHHLNPTATIVFGLCD
                     GRSTMREMSQDISAAFGVAADEVEQQVRALIRRFRGANLLCPPGTGPGDGAGGRG"
     gene            complement(4073..4357)
                     /locus_tag="VE646_03720"
     CDS             complement(4073..4357)
                     /locus_tag="VE646_03720"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HZD79134.1"
                     /translation="MIASIQQGYPTRKQRVWLKQSDQENVLYDPEHETVHLLNATAMA
                     IWVLCDGETSPDEMIDAICELSGLPRDVVLEDVRRTLEQFTEARILTWRS"
     gene            complement(4354..5040)
                     /locus_tag="VE646_03725"
     CDS             complement(4354..5040)
                     /locus_tag="VE646_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD79135.1"
                     /translation="MTEAEAVVRVLLADEQSLFREAMKIVLGSQEDLEVVGEARDGMQ
                     AVAEVERVRPDVALLDAGLPNFDGIRATALIAERAPDCQVIVLSAQEDERVLVRAMEA
                     GASGYLSKASPMSDLIEAARAVHRGEALVPPRMLGALLKRLVQRRRERDDALKRMGSL
                     TRREREVLALVADGADNEGIAQELVISPETARTHVQNVLGKLGVHSRLEAAAFVTQNG
                     IMDDLVGAGS"
     gene            complement(5175..>5474)
                     /locus_tag="VE646_03730"
     CDS             complement(5175..>5474)
                     /locus_tag="VE646_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZD79136.1"
                     /translation="AGRSGDGSAEGLWDLEGVSDGHRNAPSAEELLGSQLTDREREVL
                     ALLTKGSTSLAIARTLQVSPNTVRTHVQSIRYKLQVHSRLEAAAFAVRHGLVPVE"
BASE COUNT          784 a         2137 c         1822 g          731 t
ORIGIN      
        1 gcacgaaggc gatgagggcg ttcgagaacg cctcggcgat ccgctccgcc tggcgggggc
       61 cggtggcggt gccggtgatg tcgaggaacc catcgctttg gggatcgggg gtgacctgca
      121 cgttccccgc caacaccgag ggatcgccgg tccaccccag ctccttcgcc gcgaggtcaa
      181 ccacgtccgg cagggtggcc acccgcacca gcgcaggaac gtccaggatc ggcaccccgg
      241 ggacggtcga gctggttccg ttccctagca gcgcggtggc cgagtaccgt tgcggcgccg
      301 cccgcacggg tgccacggta gaggtggtga gccacccggc cgcggccgcc accaagacgg
      361 cggccacgat caccgcccac cgccgccgga aggccctcag atactggatg ggatccatgc
      421 tgcgctccct cgtgccgcga ccacggcgac cgccgccgcc tggttgtgga cgccgagctt
      481 ctcgaagatg cgggtcttgt gctgctcgac ggtcttcggg ctgatgccca ggagccgggc
      541 cacctgcttg ccggccatcc ccttggccac cagcgtcagc acctgtcgct cccgccgggt
      601 gaggcccctc gaaccatcga cggccccgga ctccggcgcc agggcggccg ccagacggga
      661 gaccggggcg tgcagcggca cccggatccc ttcgtcctcc gccccgtcgg tgggctgctc
      721 gatcagcacc acccggaccc cgagccgacg cagccgcgcc gcggcatccc tcgccccggg
      781 aagccgacca tccaccgcgg ccgcctgagc ccgcaccccc atggccacca gctcgtccgc
      841 gtccgtggtc acccccaccg gaaccagccc ggggaagcgg gccagcgccg ccgcgagggc
      901 ctcggccgtc agggcctcgg gatgggccac cagcaccgtc cgggagctca cggcagcctc
      961 gcctccaccg gctccgcctc cgccttggtc gggcccaccg ggagacgcat cagctccgcc
     1021 agctcggcga gcctgccctc gccggccagc ccctcgagct cgctcaggcg cgcacgcagc
     1081 aacgctcggt ccggcaccgg cgatgccgcc gccacgatcc cggggtggtc ggtggacatg
     1141 gggcgctcgt cggcatccac cagctcctcg tgcagcttct cgcctggccg gatcccgcag
     1201 atctcgatcc gcacgtcgcg ctcggggacc cgcccggaca ggtgcatcag ccgacgggcc
     1261 aggtccagga tgttgaccgg ctcgcccatc tccagcgtga agacctcgcc cccccgggag
     1321 agggccgccg cctgcagcac cagctggacc gcctcctgga cgctcatgaa gtaccgggtc
     1381 atggccggat cggtcacggt caccggcccg ccggcctgga tctgacgcag gaacgtgggg
     1441 atcacgctgc cccggctgcc cagcacgttg ccgaaccgga ccgcgcacag cacggtgccg
     1501 cgccccgaga ggctgcgcac cacgtcctcc gccagccgct tggaggcccc catcacgctg
     1561 gaggggcgga tcgccttgtc cgtggagatc aggacgaacc gctccgcccc ggaggtgacc
     1621 gccgcatcgg ccacgttggc cgtgcccagg acgttcgtgg ccagggcctc ctgtgggtgg
     1681 tcctccagca gcgggacgtg cttgtgggcc gccgcgtgga acaccacctg gggctgatgc
     1741 tccaggaaca gctccaccat ccgggtccgg tcccgaacgt ccgccaggac cgggacggcc
     1801 ttggcggagg caccgagcga gaccagcagg tcgtgcaggt gggtctcgtc cccgtccagc
     1861 aggagcaggc ggtccggctc gaacccggcc acctgaaggg cgatctcgca gccgatggag
     1921 cccccggcgc cggtgatcag cacccgcttg cctcgcagca ggcctcccac cgcttccagg
     1981 tcggtctcca cctgtcgcct gcccaacagg tcctcgatgt gcaggtcgcg gaggtcgcgg
     2041 acccccaccc gaccccccac gagctgccgg accgagggca gcacgcgcag cgagaccccg
     2101 gcctcctcgc tccggcgggc cacgtccctg atcacctcgc tggtggccga ggggacggcc
     2161 agcagcacct catccaccgc gtaccgatgg gccaaggagg ggatatcgtc gcgggtcccc
     2221 agcacccgca ccccgtgcag cgccaggcgc tggcgccgcg gatcgtcgtc caccatgccc
     2281 accggtcgca gcccgacctc gggatgtcgg agcacgtcgc tcagcagctg ggcccccgag
     2341 tcgcccgccc ccatcaccag gacccgcttg acgcggccct cgtccatcgc cacctcccgg
     2401 cgcctgaagc cgaacagccg gctctggaac cggatggcgc cgaacgccac cagggcgccc
     2461 gccgccccca tcaacgcggc ggagcggggc atcgggaccc ccagccactg gtcggccccc
     2521 atgaccagga ccgcggccgt gatcccggcc aggcccaggc ggcgggcctc ctggacgctg
     2581 gcgtagcgcc acatctgtcc gtacagcccg aacagatagt tggccagcag gtgcgccgcc
     2641 atcagcgccg ggaagaagga tcggaacccg gcccacatgt cggcggggac gtcgaagttc
     2701 aaccgcacca gcagcgcagc caggtaggcg gcgaacaccg ccagcaggtc caggaggatg
     2761 agcgggacgt cgtggcggaa cagcacgacc aaccgcccgg ggaccgtctg tcgcagctcg
     2821 gcgcgatcca cgaccgccta ccccccgttc cccgtcccgg gaccgccgtc cagcagggcc
     2881 aggaccgtcc ggatcatgtg ctcggtgccc atgagcctgg tggtgcaggg ctgagcggtc
     2941 tcgagcagcc gggccaggcc tcgcaccgcc cgccccccca gcacggggag gttcttcgtc
     3001 cgcgaggcca gctgcaccag cgcctcggcg cgcggcaacg gctccagccc cggttcggga
     3061 ccgaacagca tcacgcggtc ggccggcacc ggcacctcca cccacgcccg tgggtccggt
     3121 ccctccggtg gggcctgcag gatctccagt gcgtcgtcgg ggacttccag ccggcgcggg
     3181 accgggatca gcttcccggt gtcggggtcg atcgccacgg ccagggtccc cggcagcacg
     3241 gcgtgcgcgg cccgctgcac ccggcgtccg gcccgtgcca gcgcgggcac catgtacgag
     3301 ggcaccatgg cggtcctccc gccgccggcg accaccgccg cctccaggta gatcacgtcg
     3361 tctcgaccgg ggaacgtgag cgcctccacc tccagcagga aggcccgggc cagggtccga
     3421 aggtccatcg ttcggaccac gtcccatgtg ccctcgtaca ggatgtggaa ccgcctgccc
     3481 acccgaccgc tcgccccgct gccatcctcg accaccaggc cgtagtaggg ctcggcgtcc
     3541 ggatcctccg gaccgtccac cgcgtaggcg cccagcacgt ggtccagcca tccgccgaac
     3601 gccggcgagg tggtgcgcac cccgaaccgg gtctccccca gctggaacca ccggcacacc
     3661 acccacccga ggcggtccag cttggacatt caggtgctct tgggcttctt ctgacgggcc
     3721 tgctcccgct cgtccaggag cgcccccgcg gtcggggccc ggctcatccc cgccccccgg
     3781 cgccgtcgcc gggcccggtc cccggaggac acagcaggtt cgcgccgcgg aagcggcgga
     3841 tcagcgcgcg cacctgctgc tcgacctcgt cggccgccac cccgaaggcc gcggagatgt
     3901 cctgggacat ctcgcgcatc gtgctccgcc cgtcgcacag gccgaacacg atggtcgccg
     3961 tggggttgag gtgatgcagg tccccggtct cctcgtcgta gatcacggcc tcgccgtcca
     4021 gctcgaccac ggtgaggtcg gagcgcatca tgggccggct cacgggcatg gctcaggacc
     4081 tccaggtgag gatgcgggcc tcggtgaact gctccagggt cctgcggacg tcctccagga
     4141 ccacgtcacg gggaaggccg gacagctcgc agatcgcgtc gatcatctcg tcggggctgg
     4201 tctcgccgtc gcacaggacc cagatcgcca tggccgtggc gttcagcagg tgcacggtct
     4261 cgtgctcggg gtcgtacagg acgttctcct ggtcggactg cttgagccag acgcgctgct
     4321 tgcgcgtggg atagccctgc tggatcgatg cgatcacgat ccggccccca ccaggtcgtc
     4381 catgatgccg ttctgggtca cgaaggccgc cgcttccagc cgggagtgca cgcccagctt
     4441 gccgagcacg ttctgcacgt gggtccgcgc ggtctccggg ctgatcacca gctcctgggc
     4501 gatgccctcg ttgtcggcgc cgtcggcgac cagggccagg acctcgcgct cacggcgggt
     4561 gaggctcccc atccgcttga gggcgtcgtc ccgctcccgg cgccgttgga ccagccgctt
     4621 cagcagggcc cccagcatcc ggggcggcac cagggcctca ccccgatgca cggcacgggc
     4681 ggcctcgatg agatcgctca tgggcgaggc cttcgagagg tagccgctgg cccccgcctc
     4741 catggcccgg accaggaccc gctcgtcctc ctgggcgctc agcacgatga cctggcagtc
     4801 gggcgctcgc tcggcgatca aggcggtggc ccggatccca tcgaagttcg gcagcccggc
     4861 gtccaacagc gccacgtcgg gacgcacccg ctccacctcg gccaccgctt gcatgccgtc
     4921 acgggcctcc ccgacgacct ccaggtcctc ctggctgccg agcacgatct tcatggcctc
     4981 gcggaacagg gactgctcat cggccagcag gacgcgtacc actgcttcag cctcggtcac
     5041 gatgagctcc cccaaggggt cggcaggacg acgctagcca cccctccctg cgcacgcacg
     5101 cgcatccggt cgtaggaaac ctacggtgcc tcccgtacga gaacctcacc ctcccccgga
     5161 cgcggctccg gcgatcactc cacgggcacg aggccgtggc ggaccgcgaa ggcggccgcc
     5221 tccagccggg agtgcacctg cagcttgtac cggatgctct gcacgtgggt ccggaccgtg
     5281 ttgggcgaga cctgcagcgt ccgggcgatc gccaggctgg tggagccctt ggtcaggagc
     5341 gccaagacct ctcgctcgcg gtcggtcaac tggctgccca acagctcttc ggcgctcggc
     5401 gcgttccgat ggccgtcgct tacgccctcg aggtcccaga gcccctcggc ggagccgtcg
     5461 ccggaccggc cggc
//
LOCUS       DASQQQ010000089         2820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_144388, whole genome shotgun sequence.
ACCESSION   DASQQQ010000089 DASQQQ010000000
VERSION     DASQQQ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144388"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1560)
                     /gene="nadB"
                     /locus_tag="VE646_03735"
     CDS             complement(<1..1560)
                     /gene="nadB"
                     /locus_tag="VE646_03735"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HZD79137.1"
                     /translation="MRVSPEVRETDVLVLGSGIAGLSYAIKAAAHGSILLLTKKEDTE
                     ANTNYAQGGIAAVMDPGDSFAAHVQDTLTAGAGLCHEEAVQVLVENGPARVGELIDYG
                     VAFSYRGEAPERLSLAREGGHSARRIVRADDLTGRAIEKGLLAEASKAGVEILEHWFA
                     LDLWMTGEGADRRCHGAVVVDRHSGELVAVRARVIYLATGGCGQVYSYTTNPAIATGD
                     GVAMAWRAGAAVANMEFIQFHPTALYPVEEEPFLISEAVRGEGAVLRDQEGREIMRGK
                     EGGDLAARDVVARAIDTEMRSSGRPHVWLDCGPIRPDRFAERFPNILETCRARGFEPP
                     GEWLPVVPAAHYACGGVRTDLDARTDVRDLLAAGEVACTGVHGANRLASNSLLEAVVF
                     ADRAAETTRRLLEEPAPAVDPPPNRQPRDGMDPGPLRERVREVLWTGAGIVRSDPGLA
                     EARARLDELAEGLSVEPIDPAAAEAANLHLVGSLIVASATLRKESRGLHFTLSYPESD
                     PAYRRDTVLVSE"
     gene            complement(1557..2675)
                     /gene="lpxK"
                     /locus_tag="VE646_03740"
     CDS             complement(1557..2675)
                     /gene="lpxK"
                     /locus_tag="VE646_03740"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HZD79138.1"
                     /translation="MTERSGPVIDEGARIYGWARRTLYGEGGRTGLAARTAARPLGWT
                     WNRLARRRLERRRTAERLPGPALGVGNVTVGGGGKTSLVMWLIEAGLPEEARPAVLLR
                     GYGRTGPGVWVLKPGDLSVGADVVGDEPALLARSGAWVGVARDRCRAAQAVAARRRPH
                     VFILDDALQNRRVARVLDLVVFTSEDLLAPARCLPAGPLRQGPAWQPALGVWLVVGMD
                     PRERVWPEGTIGRAFAGWWHELAGFVAEWRDAGTISLSAWRAGREIPWEPEDEVVAVT
                     GVAKPESVARFAADVGFPVTRVAAFPDHHRFREVEIRFLIEEHPGAAFVITEKDAVKV
                     DAAWFGDRPVGVLRRRLAPVDPEPLRDLVREAIGWPHR"
BASE COUNT          429 a         1104 c          902 g          385 t
ORIGIN      
        1 ttcggacacc agaacggtgt cccgtcgata ggcgggatcg gattcgggat acgacagtgt
       61 gaaatgtaag ccccggctct ccttgcgcaa cgtcgccgat gcgacgatca agcttcccac
      121 caggtgcagg ttggcggcct cggctgcggc cgggtcgatg ggctcgaccg agaggccttc
      181 ggcgagctca tccagccggg cgcgggcctc cgcgagcccc ggatcgctgc gcacgatccc
      241 ggcccccgtc cagagcacct cgcgcacccg ctcgcggagg gggccgggat ccatcccatc
      301 gcgcggctgg cggttgggcg gaggatcgac cgccggggcc ggctcctcga gcagccgccg
      361 ggtcgtctcg gcggcccggt cggcaaacac aaccgcctcg aggagcgagt tgctggccag
      421 ccggttggct ccatggacgc cggtgcaggc cacctccccg gccgccagca ggtcccgcac
      481 atcggttctg gcatcgagat ccgtccgcac gcccccacac gcgtaatgcg ccgccgggac
      541 gacgggaagc cactccccgg ggggttcgaa gccgcgtgcc cggcacgtct cgaggatgtt
      601 cgggaagcgc tcggcgaacc ggtccgggcg gatgggcccg cagtcgagcc agacgtgagg
      661 ccggccgctt gagcgcatct cggtgtcgat ggcgcgggcc accacgtcgc gcgccgccag
      721 gtccccccct tccttgccgc gcatgatctc ccggccttcc tgatcccgca gaaccgcccc
      781 ttcaccgcgg accgcctcgc tgatcagaaa cggctcctct tcaacgggat acagcgctgt
      841 cgggtgaaac tggatgaact ccatgttggc gacggccgcc ccggcgcgcc aggccatcgc
      901 aaccccatcc ccagtggcga tggccgggtt ggtcgtgtag ctgtagacct gcccgcaacc
      961 cccggtggcc agatagatca cccgagcgcg gacggccacc agctcgccgc tgtgccggtc
     1021 gacgaccacc gccccgtggc acctccggtc cgccccttcc ccggtcatcc acagatcgag
     1081 cgcgaaccag tgctcgagga tctcgacacc ggccttcgag gcctcggcca gcaacccttt
     1141 ctcgatcgcc cggccggtga ggtcgtcggc gcgcacgatg cggcgggccg agtgcccgcc
     1201 ttcgcgggcc aaggacagcc gctcgggagc ctccccacgg tagctgaagg cgaccccgta
     1261 gtcgatcagc tcgcccaccc gggctggccc attctccacc agtacctgca cggcttcctc
     1321 gtggcagagc ccggctcccg ccgtcaaggt gtcctgaaca tgggcggcga acgagtctcc
     1381 gggatccatg accgccgcga tgcccccctg ggcgtagttg gtgttggctt cggtgtcttc
     1441 cttcttggtc agcaggagaa tgctcccgtg ggctgccgcc ttgatggcgt acgaaagtcc
     1501 cgcgatcccg ctccccagga cgaggacgtc cgtttcgcgg acctccggcg aaaccctcac
     1561 cggtgtggcc agccgatcgc ctcgcggacg aggtcccgga gcggctcggg gtcgaccggt
     1621 gcgagccgac gccggagaac ccccacgggt cgatccccga accaggcagc gtcgaccttg
     1681 acagcgtcct tctcggtgat cacgaaggct gcccccggat gctcctcgat caggaaccgg
     1741 atctcgactt ccctgaagcg gtgatgatcg gggaaggccg ccacccgcgt caccgggaag
     1801 ccgacatcgg cggcgaaccg ggcgacggat tcgggcttcg ccaccccggt gacggccact
     1861 acctcgtcct ccggctccca cgggatctca cgacccgcac gccaggcgct cagcgagatc
     1921 gtgcccgcgt cccgccactc cgccacgaag cccgcaagct cgtgccacca ccccgcgaag
     1981 gcacgtccga tggttccctc cggccacacc cgctcgcgcg gatccatccc gacaaccaac
     2041 cacaccccca gcgccggttg ccacgccggt ccctgccgga ggggacccgc gggaaggcac
     2101 cgggcaggcg ccaagagatc ctccgaggtg aagacgacga gatcgagcac cctggccacc
     2161 cgccggttct ggagcgcgtc gtccaggatg aagacgtgcg ggcgccgtct cgccgccacc
     2221 gcctgggccg cccggcagcg gtcccgggcc accccgaccc acgccccgga ccgcgccagc
     2281 agcgcgggct cgtcgcccac gacgtccgcc ccgacggaca aatcccccgg cttgagaacc
     2341 cacacaccgg gaccggtccg gccatatccc cgcaggagga ccgctggacg cgcctcttcc
     2401 ggaagcccgg cctcgatcag ccacatcacc aggctcgtct ttcccccgcc ccccacggtg
     2461 acgttgccga cgcccagcgc cggccctggg agccgctccg ccgtgcgccg gcgctccagg
     2521 cgtcgacggg cgagacgatt ccaggtccat cccagaggac gcgctgccgt gcgcgccgcg
     2581 aggccggtcc ggccgccttc cccgtagagg gtcctccgcg cccagccgta gatccgggcc
     2641 ccctcgtcga tcaccgggcc gctccgctca gtcacgggac caccaccggc gcagccgccg
     2701 gctcgagccc ttcgccgcga agctcggcct cgagacgcag gtgaagcgcg cacaacgctc
     2761 tctcgagatt ggcgctcgcg caccaacccg cctgcctgtc catgaccggg ggaatccaaa
//
LOCUS       DASQQQ010000090        15296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_115918, whole genome shotgun sequence.
ACCESSION   DASQQQ010000090 DASQQQ010000000
VERSION     DASQQQ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15296
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115918"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..565
                     /locus_tag="VE646_03745"
     CDS             182..565
                     /locus_tag="VE646_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD79139.1"
                     /translation="MALRVLVVDDHPAFRRALTAALRMVRDIEVAGEAGGGIAAVDQA
                     NDIHPDVVLMDLSMPDIDGIEAMRRIRRSKPNLPVVFLTAHADPGIEKEAREAGASGF
                     LAKGIGLDELVTVIHEAADGVVRIP"
     gene            complement(592..2100)
                     /gene="lysS"
                     /locus_tag="VE646_03750"
     CDS             complement(592..2100)
                     /gene="lysS"
                     /locus_tag="VE646_03750"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZD79140.1"
                     /translation="MTEDDVTGREREVLRARRSSRERLGDRAFALSLQEVLGAAEPTS
                     SGAIRQRHGAMPPDRVEDVPYAVAGRVVLRRDFGKLAFLVLRDRDGDIQVVCDRGDLP
                     QADFELLEEVDLGDIVAAHGRVGTTRKGELSIFAERLAMLSKALRPLPEKWHGLRDPE
                     LQQRYRYLHLATDDRARRYAIARAQVLRTLRRELDRRGYVEVETPVLQAIAGGALARP
                     FTTHHNALDLDLKLRISLELYLKRLLVGGLERVYEIGRNFRNEGVDREHNPEFTMLEL
                     YEAYGDYETMMRIAEALITACAEAVRGSRSLEFRARGLDLEPPWRRVTVLGSVGDAIG
                     RPVSLDTPDLAALADEHGVYVDPAWGPGKIAQELFEKLVEPTLWEPTFVCDFPREVSP
                     LARSHRTEAGLTEHFDLVVGGVELATAFSELTDPDEQRVRFELQASQRAAGEEETHPY
                     DQDFLTALEHGMPPAGGIGIGIDRLLMVLTDAPSLRDLILFPHLRPDEGPEG"
     gene            complement(2093..2869)
                     /locus_tag="VE646_03755"
     CDS             complement(2093..2869)
                     /locus_tag="VE646_03755"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HZD79141.1"
                     /translation="MLLAVDVGNTESVLGVFRDAELITHWRMATVPERTADELALVFG
                     GFLQHEQIAFSDLTGIVISSVVPAATDALREMVARYLSFHPVVLEPGVRTGMAILIDN
                     PREVGADRIANSLAAYARYGGPCIVLDFGTATNFDVVSAAGEYLGGVIAPGLQVAASS
                     LYAAAARLTRVELRAPRSVVGKNTVEAVQSGLILGAAAEVDGIVQRIEEEIGPAKVVA
                     TGGLAGIVVPHCRTSIDQEPWLTLEGLRLVFERNVGIDGD"
     gene            complement(2968..3819)
                     /gene="panC"
                     /locus_tag="VE646_03760"
     CDS             complement(2968..3819)
                     /gene="panC"
                     /locus_tag="VE646_03760"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HZD79142.1"
                     /translation="MEMIRAVAELRAEGDRARAEGSLVGLVPTMGALHEGHASLIRRA
                     RQECRAVIVSIFVNPLQFGSGDELRSYPRDEARDLRVCEGLGVDLVFAPSAGAMYPEG
                     EPTVALDPGPLGRRLEGASRPGHFRGVLTVVAKLFHAAGPCRAYFGEKDAQQLFLVRR
                     MVRELDFPVQVVACPTVREADGLARSSRNVLLSAQERQAAGCLFLALSEAAELSRSGE
                     RDGGRIVAVMAREVGATPLARLDYAAVVDEDTFEEVRRAEGSMRALVAARFGAVRLID
                     TLRLPTR"
     gene            complement(3810..4673)
                     /locus_tag="VE646_03765"
     CDS             complement(3810..4673)
                     /locus_tag="VE646_03765"
                     /inference="COORDINATES: protein motif:HMM:NF019603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HZD79143.1"
                     /translation="MDVAVVGAGRVGTAMAVLLGRAGHRIVGLAGRSATIQRAATFLP
                     GLPVTDPVRAAEGADVVLIATPDDAIASIATSLAGAGVLSRGRAVVHVSGATSLDALA
                     AGRTAGATVLSVHPLQTFPTVEAALERMPGVPIAVTATDEEGATLGERLAVDVGGRPF
                     RLADEAKPLYHAAAVFASNYLVAVTALGQAVGRAAGLEDPVALMAALQRTTLENVISL
                     GPATALTGPAVRGDAGTIAGHLEALSGSVPSAVDAYVALARTAIDLGERSGRLPPGRR
                     RAVERVLERWR"
     gene            complement(4693..5319)
                     /locus_tag="VE646_03770"
     CDS             complement(4693..5319)
                     /locus_tag="VE646_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79144.1"
                     /translation="MSVDVVHVSTEVPERAVTLPHLCGGIVSLGRDINTTYFTARSEG
                     SVDTYSVGIDAVHPVLSGYRLLGVSPAADFLATPAGGRSTAGAGTSLFWLGTGGPIPY
                     GTAHRPLRVDRVLAWSRDGSQAAVLGWDGVVHGVFVLDASAGSDERRLARFVGVVPGR
                     STFATFASDGTLYLSLDGVLVRYADGVTRQVALPAGVPQPMGPILWLP"
     gene            complement(6123..6629)
                     /gene="folK"
                     /locus_tag="VE646_03775"
     CDS             complement(6123..6629)
                     /gene="folK"
                     /locus_tag="VE646_03775"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HZD79145.1"
                     /translation="MEVPAFIGLGSNLGERLANLQAAVDLLDESPLVRVARSSRVYET
                     APVGGPRQPDYLNAVLEVRTELDPGALLRTCHQVEAALGRVREERWGPRTIDLDILGY
                     DRAEVQEPDLTIPHPRMHERAFVLMPLLELTADPLLPGGRSVASLRLPPNALTEVRPF
                     APPLRVTP"
     gene            complement(6641..6997)
                     /locus_tag="VE646_03780"
     CDS             complement(6641..6997)
                     /locus_tag="VE646_03780"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HZD79146.1"
                     /translation="MSTSRLFLTGIDVEGRHGANAGEKDRPQAFVVDLDVEVAVSSDT
                     LEGTADYRTLIRTAREVVQGTSFDLLESIATAVAEAVAARSGVVRATAIVHKPGAAIS
                     SGVQGVAAAATAEPTG"
     gene            complement(6994..7887)
                     /locus_tag="VE646_03785"
     CDS             complement(6994..7887)
                     /locus_tag="VE646_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZD79147.1"
                     /translation="MSDLQKRQVELSVGRISYLEEGDPEEPAIVLLHGYPTSSYLWRE
                     FVPAFAPWFHVIAPDLVGSGDSDKPPDLPLHVRAQTAYLVELLQALGIGSCAAVGHGA
                     GGGIAQLLALEGRVRAMVLVDSVAFDAWPTPLVREVQRRQPSEGLPEGFLPRWLRSGM
                     GHPERLKAETLREYLRPFQGPEGAAALLRLVRALDGRGLAGREGELARLDIPILLLWG
                     EDDAYLPVDIAERLAEAVPTASLAVLPGCSHFLPEDAPETIAPLMFEYLRSRYLGRSH
                     EHRGGPVVVELQRKPFEGEAG"
     gene            complement(7884..8735)
                     /gene="folP"
                     /locus_tag="VE646_03790"
     CDS             complement(7884..8735)
                     /gene="folP"
                     /locus_tag="VE646_03790"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZD79148.1"
                     /translation="MPEGTALIWRCGARTIECGGRTLLMGVLNVTPDSFSDGGRFLDP
                     DAALGHGLAMMKDGADMVDIGGESTRPGSDPVPLEEELRRVLPVVQALASRTAVPLSI
                     DTRHAAVAEAALRAGAVIVNDISGGREPGMFDVVKAAGAGLVLMHMRGDPKTMQEAPT
                     YTDVVGEVKDYLARRLEAAIFAGLSPEQLVVDPGIGFGKDLEHNLELLRRLDALGDLG
                     RPILVGPSRKRFIGTLLDLAEDERVEGTAGVAAWAATHGASIVRVHDVKQISRVIRLV
                     DAIALGR"
     gene            complement(8780..9349)
                     /gene="folE"
                     /locus_tag="VE646_03795"
     CDS             complement(8780..9349)
                     /gene="folE"
                     /locus_tag="VE646_03795"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018707158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HZD79149.1"
                     /translation="MSFDRQRIEEGIRMILAGIGDDPDRDGVKDTPRRVADMYEEIFA
                     GIGVDARSLVTVIPGADFDEMIMVKDIPLYSSCEHHLIPFVGKAHVAYVPSKEGRITG
                     LSKVARVVDMLAKRPQVQERLTTQIADAIDDALEPRGVFVMIEAEHLCMTMRGVKKPG
                     SRTVTSAVRGLFRTDARTRQEALSHIGKA"
     gene            complement(9408..10070)
                     /locus_tag="VE646_03800"
     CDS             complement(9408..10070)
                     /locus_tag="VE646_03800"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZD79150.1"
                     /translation="MSERWATFDCYGTLIDWLGGIRMTLSELFPEHDAELLLGAYHEI
                     EPEVQRGRSISYRQVLSETLERLAHREGLELAADDRAALAESLPTWPPFPEVPPALDE
                     LRHRGWRIAILSNTDPDLLDASLSLISVPVDARITAAEAGSYKPSPGHWETFFERTGA
                     DRDRHAHVAASLFHDIEPCAAMGLRSIWINRLGESSQLSRAAELPDLDDLPDTLDRLV
                     PA"
     gene            complement(10112..11950)
                     /gene="ftsH"
                     /locus_tag="VE646_03805"
     CDS             complement(10112..11950)
                     /gene="ftsH"
                     /locus_tag="VE646_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZD79151.1"
                     /translation="MFVLLTNHGSQGGKLSYSQYQSFVDQGKVRTATILEGDQTVRGE
                     LTDNTSYQVAYPEASQESLIKELGAQNVDVNVDPQKGSALLGFLVQLIPVILIIGAFI
                     WIMNQSQGGGGRVMQFGKARAKVVSKDQPKTTFTDVAGVDEAIEELQEVKEYLQNPAK
                     FQAMGAKIPKGVLLFGPPGTGKTLLARAVAGEAGVPFYSISGSDFVEMFVGVGAARVR
                     DLFDQAKANAPAIVFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFDQRTAVI
                     LMAATNRPDILDPALLRPGRFDRHVTIDRPDLEGRKGILKVHARGKPFDGSVELDVIA
                     RRTPGFTGADLSNVINESALLAARWGRKAIGMKEVEEAIDRVMAGPERKTRVMNEREK
                     RMIAYHEGGHALVAHVLPNTDPVHKISVIPRGRALGYTLTLPQEDKFLMTREELSDEL
                     AMLLGGRVAEELIVGDITTGAANDIERATKVARQMVTEYGMSDTIGPLTLGQKQHEVF
                     LGRDFASQPDYSDQVAFEIDNEVRQMIDQAHDEALEILQERRAKLDEIAAALMERETI
                     DKDEVERILAEVPKRAQRDAAVRGAGLAVSRRAVRAQRDQGPAPLR"
     gene            complement(12151..12552)
                     /locus_tag="VE646_03810"
     CDS             complement(12151..12552)
                     /locus_tag="VE646_03810"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZD79152.1"
                     /translation="MNRGRIALVLLVPVLTAACSSSPTQSGTGTTPTTGIRGVVLLGP
                     VCPIVSAESPCPPRPLADTTVEVRNDRGQVVATAVTDARGRFGVQVPPGRYQVRAEPD
                     GAGPRIGHLVSVTVPEGGSAQVKVPVDSGIR"
     gene            complement(12607..13224)
                     /gene="hpt"
                     /locus_tag="VE646_03815"
     CDS             complement(12607..13224)
                     /gene="hpt"
                     /locus_tag="VE646_03815"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HZD79153.1"
                     /translation="MEGRVALSEPNGTSSAPLEAPFAEEYERHIQKVLIGREEIQAKL
                     AELGAAITHDYAGTDPLLVGVLKGAFVLMADLARHIRMPVDFDFMAVASYGASTQTSG
                     VVRILKDLDHDIEGRHVLLIEDIVDSGLTLNYLLKNLRGRRPASLEVCALLHKVGIQQ
                     VHLDLRYVGFEIPPEFVIGYGLDFAEKYRNLPYIATLKPEAYRDL"
     gene            complement(13239..14237)
                     /gene="tilS"
                     /locus_tag="VE646_03820"
     CDS             complement(13239..14237)
                     /gene="tilS"
                     /locus_tag="VE646_03820"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZD79154.1"
                     /translation="MARPPTVARVLHRVRATVHEHGMFLPGETVMVAVSGGPDSMCLL
                     HSLVELRRLLKIRLEVFHLDHRLRPGSADDAAYVERAARRLRLPFHLRVAETVPPAGA
                     SVEDWAHRVRLRALALTMREAGASRAAIGHTLDDQVETVLIALLRGGGLDAVAGLRPV
                     HGPYVRPLIDVTRDEVNAFCRARHLRPRIDPTNRDVRLFRNAVRLRALPAIEDATRRD
                     VRAPIARTSALLRRDAEELERRATEAMVEFLEEVPEGIRLDALALTSLPWAIGSRVAR
                     SAVYRCGVLPSADDVDAILDLSAGRPGRKRHLTGGLVAARRKEYVSLSRSSPESRG"
     gene            complement(14237..>15296)
                     /locus_tag="VE646_03825"
     CDS             complement(14237..>15296)
                     /locus_tag="VE646_03825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HZD79155.1"
                     /translation="VDWSMGSQAAKLVDWGVAANVGRRVGGRGPVLQAIERARLREDL
                     SATVPHAEELVSGFTGLAAGGFRSRAWVMSRGEWVATNLRGLQRLLEPLARRMLAENA
                     RRSDLRRKALGAQIGVLLGYVSKKVLGQYDVFLPPDDEGLLYFVGPNLAETERRFALP
                     AHDFRMWVAIHEVTHRVQFGATPWLRGYLNRMVNTYLDTVQLDAREMLAQLRRAVAEA
                     RVSTEWRSANLLMFLMTPEQRDLFHRMQAMMSLLEGHASFVMDEVARDHVRDLPRLRR
                     SLKERRSVSGMEKTFQRAIGFDSKIRQYSSGEAFVRAVHGRGGMRALNLVWLDEANLP
                     TFQEIAQPERWLARVAGG"
BASE COUNT         2361 a         5580 c         5207 g         2148 t
ORIGIN      
        1 cggcagcggc cccggttcac ctcccctccg tgagcagccg tagggcacag ggccgccccc
       61 gcatatgacc cattcggact agcgaagttc ccccggatgg cccgttgttc gttcgcccaa
      121 cgctcgggga agatggcggc aggcgatggt gccttcccct ggtcttccct gtaaggagac
      181 gatggcgttg cgggtgctgg tcgtggacga tcacccggcc ttccgtcggg cgttgaccgc
      241 tgccctgcgg atggttcggg acatcgaggt ggcgggcgag gcggggggag gcattgcggc
      301 cgtggaccag gccaacgaca tccacccgga cgtggtcctg atggacctct ccatgccgga
      361 catcgatggg atcgaggcca tgcgccggat ccggcgatcc aagccgaacc tgccggtggt
      421 gttcctgacg gcccacgccg atccgggcat cgagaaggag gcccgtgagg ccggcgcctc
      481 gggcttcctg gccaagggga tcggtctgga cgagctggtc accgtgatcc acgaggccgc
      541 cgacggggtg gtccggatcc cgtaggcgcc agctccccga gtcgtcgctc ctcacccctc
      601 gggcccctcg tcgggtctga ggtgtgggaa gaggatcagg tcccgcagcg aaggcgcgtc
      661 ggtcagcacc atcagcaacc ggtcgatccc gatcccgatc cccccggccg ggggcatacc
      721 gtgctcgagc gcggtcagga aatcctggtc gtagggatgg gtctcctctt ccccggcggc
      781 ccgctgcgaa gcctggagct cgaagcggac gcgctgctcg tcggggtccg tgagctcgct
      841 gaacgccgtg gccagctcga cgccgccgac caccagatcg aagtgctcgg tgaggccggc
      901 ctcggtccgg tgggacctgg ccagcggcga gacctcgcgg gggaaatcgc agacgaacgt
      961 gggctcccac agggtcggct cgaccagctt ctcgaacaat tcctgggcga tcttccccgg
     1021 accccaagcg ggatccacgt agacgccgtg ttcgtcagcg agcgccgcca gatccggcgt
     1081 gtccagggac acgggccttc cgatcgcgtc gcccacgctc cccagcacgg tgacccggcg
     1141 ccagggaggc tcgagatcga gcccgcgcgc gcggaactcc agcgaccggc tcccgcggac
     1201 ggcctcggcg cacgcggtga tcagagcctc ggcgatccgc atcatggtct cgtagtcccc
     1261 gtaggcctcg tacagctcga gcatcgtgaa ctcggggttg tgctcccggt cgaccccctc
     1321 gttgcggaag ttgcgcccga tctcgtacac gcgttccaga ccccccacca ggagccgctt
     1381 caggtacagc tcgagcgaga tccggagctt gaggtccagg tccaaggcgt tgtgatgggt
     1441 ggtgaacggc ctggccagcg caccgcccgc gatggcctgg aggacgggcg tctccacctc
     1501 gacgtagccg cgcctatcca gctcgcggcg caaggtccgc aggacctggg cccgcgcgat
     1561 cgcgtaccga cgggcgcgat cgtcggtggc caggtgcagg taccggtacc gctgctgcag
     1621 ctcgggatcg cgcaggccgt gccacttctc gggcagcggt cggagcgcct tggagagcat
     1681 ggccaggcgc tccgcgaaga tcgacagctc ccccttcctc gtggtcccca cgcggccgtg
     1741 agccgccacg atgtccccga ggtccacctc ctcgagcagc tcgaagtcgg cctggggcag
     1801 gtccccccgg tcgcacacca cctggatgtc gccgtcccga tcccgcagga ccaggaaggc
     1861 gagcttgccg aagtccctgc gcaggaccac gcggcccgct accgcgtagg gaacgtcttc
     1921 gacccggtcg gggggcatcg ccccgtggcg ttgccggatc gcgcccgagg acgtgggttc
     1981 ggcagcgccc agcacctcct gcaggctaag ggcgaaggct cggtcgccca ggcgctcgcg
     2041 gctgctgcgg cgggctcgca ggacctcgcg ctctcgtccg gtgacgtcgt cctcagtcac
     2101 cgtcgatccc cacgttgcgc tcgaagacca gccgcagacc ctcgagcgtc agccagggct
     2161 cctgatcgat cgacgtgcgg cagtgaggca ccacgatgcc ggccaggccg ccggtcgcca
     2221 ccaccttggc cgggccgatc tcctcctcga tccgctgcac gatcccatcg acctccgccg
     2281 cggcccccag gatgagcccc gactgcaccg cttcgacggt gttcttgccc accacgctcc
     2341 tcggcgcccg gagctcgacc cgggtcagcc gcgccgccgc cgcgtacagg ctcgaggccg
     2401 ccacctggag cccgggggcg atcaccccgc ccaggtactc cccggccgcc gagaccacgt
     2461 cgaagttggt cgcggtcccg aagtccagca cgatacacgg ccctccgtag cgcgcgtagg
     2521 cagccaggga gttggcgata cggtccgctc ccacctcgcg agggttgtcg atcaggatgg
     2581 ccatccccgt gcggacaccc ggctcgagca ccaccgggtg gaacgacagg taccgggcca
     2641 ccatctcccg cagggcgtcc gtcgccgccg gaaccaccga ggagatcacg atcccggtga
     2701 ggtcggagaa ggcgatctgc tcgtgctgga ggaacccgcc gaacaccagg gccaactcgt
     2761 cggcggtgcg ctccgggacc gtcgccatcc gccagtgcgt gatcagctcg gcgtcacgga
     2821 acacgccgag gacggactcc gtgttcccga catccacggc cagcagcacc gcggctcctt
     2881 ccggttcgat cgtggcccgc gggcggggct cggggcgctc ggaccgcgtc atcctgtcac
     2941 gctcgacggc cggccgacac gtgcgcctca gcgggtcggc agccggagcg tgtcgatcag
     3001 ccggacggcg ccgaaccggg cggcgaccag cgctcgcatc gacccctcgg cccggcggac
     3061 ctcctcgaac gtgtcctcgt ccaccaccgc cgcgtagtcc aggcgcgcga gcggcgtggc
     3121 gcccacctcc cgcgccatca cggccacgat ccgcccccca tctcgctctc ccgagcggga
     3181 cagctcggcc gcctcggaga gcgccaggaa caggcacccg gccgcctgtc gttcctgggc
     3241 ggagagcagg acgttgcggg aggagcgggc gagcccgtcg gcctcgcgga ccgtgggaca
     3301 ggccacgacc tgcaccggga agtcgagttc tctcaccatc cgacggacca ggaacagctg
     3361 ctgcgcgtcc ttctcaccga agtaggctcg gcagggcccg gccgcgtgga agagcttcgc
     3421 gaccacggtg agcacgccgc ggaagtgacc gggccgagag gcgccctcga gccgccgccc
     3481 gagtggcccc gggtccagcg ccaccgtggg ctcgccctcc gggtacatcg ctcccgccga
     3541 gggcgcgaag accaaatcca cccccaaccc ctcgcagacc cgaaggtccc gagcctcgtc
     3601 ccgcgggtag ctgcgaagct cgtcgcccga gccgaactgc agcgggttca cgaagatcga
     3661 gacgatcacc gcccggcact cctgtcgagc ccggcggatc agcgaggcat gcccctcgtg
     3721 cagcgccccc atcgttggca ccagccccac caggctaccc tcggcccgcg cccgatcccc
     3781 ctccgcccgg agctccgcga cggcgcggat catctccatc gctcgaggac ccgctcgacg
     3841 gcccgcctcc gtcccggagg cagccgtccg gacctctccc ccagatcgat cgccgtccgc
     3901 gccaaggcca cgtaggcgtc gaccgcgctg ggcacggatc ccgacagcgc ttccaggtgc
     3961 ccggcgatcg tccccgcgtc accacgcacg gccgggccgg tcagggcggt cgcgggtccc
     4021 agcgagatca cgttctccag tgtggtccgt tgcagcgcag ccatcaacgc gacaggatcc
     4081 tctaatcctg cggcccgacc gaccgcctgg cccagggccg tcacggccac caggtagttc
     4141 gaggcgaaga ccgcggcagc gtggtagaga ggcttggcct cgtccgccag ccggaagggc
     4201 cgccctccta cgtccaccgc cagccgctcg ccgagggtag ctccctcctc gtccgtcgcc
     4261 gtgaccgcga tggggacccc cggcatccgc tccagcgctg cctccacggt ggggaaggtc
     4321 tgcagcggat gcaccgagag caccgttgct cccgcggtcc tcccggcggc gagggcatcc
     4381 agggaggtcg cccccgagac gtggaccacg gctcgaccgc gcgacagcac gccggcccca
     4441 gccaaggacg tggcgatgga cgcgatggcg tcgtcggggg tggcgatcaa cacgacatcg
     4501 gcaccctcgg cagcgcgcac cggatcggtg accggcagtc cgggcaggaa ggtggcggct
     4561 cgctggatcg tggccgaccg gccggccaga cccacgatcc ggtggcccgc ccgtccgagc
     4621 agaacggcca tcgcggtgcc cacgcgcccc gcacccacca cggctacatc catcgccgcc
     4681 gagtatccca actcagggga gccagaggat cggacccatg ggctggggaa cgccagcggg
     4741 cagagcgacc tgccgggtga caccgtccgc gtaacgcacc agcacgccat ccagggacag
     4801 gtacagggtc ccatcgctgg cgaacgtggc gaaggtggac cgtcccggca ccacacccac
     4861 gaaccgagcg agccgtcgtt cgtcgctgcc ggcgctcgcg tcgagcacaa agaccccgtg
     4921 cacgacccca tcccacccca ggacggctgc ctgggagcca tcacgggacc aggccagcac
     4981 gcggtcgacc cgcagcggtc gatgggcggt cccgtatggg atcggcccgc ccgtccccag
     5041 ccagaacagc gatgtcccgg caccggcggt cgaccgtcct ccggccggtg tggccaggaa
     5101 atcagcggcc ggtgagacac cgagcagccg gtagccggac aggaccgggt ggaccgcatc
     5161 gatcccgacg ctgtaggtgt ccacggatcc ctccgagcgg gcggtgaagt aggtggtgtt
     5221 gatgtcccga cccagggaca cgatgccgcc acagaggtgc ggaagcgtca cggcgcgctc
     5281 ggggacctcg gtggagacgt ggaccacgtc gacactcacc cggcggcacc cgtctcggga
     5341 gcccccctgg tgggcgagca cgaccacgtc cccgcggggc gcccaggcgg ccatgtcccc
     5401 acggcccaag ggacggggcc ggtcggaggg gcgtgtgccg cgcaggacga acgtgcggac
     5461 gccgccgttc ctgtcccggg cggtcagacc gacccaccgg gtaccggcca tcgatgcgtc
     5521 cgacagggcc agtagatgtg ggacccgggg gccccgcatg gtccagccct cggctaggtc
     5581 gagggtccac agcgactggg ctccgtgctg cccacgagcc gcatacaaga gcgcaccgag
     5641 cagaggcgat accggagggg tcgtggcgat agggggagag gcacggtgct cgggtccggc
     5701 ggcggtgtgg gccgctggga tcagcgtcca cacgagggcc agcgccccca ccaccgcgac
     5761 cagacccagg accagccgcc acgggccccg acctggagcg tcggacgatg ggccagcggc
     5821 cgcgccggtg tccgggtggg cgtggggatc aagatcggtc cacgtttgtg gctccgagcg
     5881 gaggggtggg ttcgagctga ccgggtcgga gggcgcaggg ggatccgagc ggccgatcgc
     5941 gttcgggccc ggggtcctgg gtgggggcgg cttggcacgg ccaccgggcg gctcggcacg
     6001 gccaccggag cgcggcccct ccgagggctc cacggcccga cggtacaggg acgtcccgca
     6061 ccgagggcag tccggatcct cagaatcccc ctcccagtca caacggaagc acgccgcgat
     6121 gctcacgggg tcactcggag gggtggagcg aacgggcgga cctcggtcag cgcgttcggt
     6181 gggagccgga gcgaggccac cgacctaccg ccgggcagca gggggtcggc ggtgagctcg
     6241 agcagcggca tcagcacgaa cgcccgttcg tgcatccgcg gatgagggat ggtcaggtct
     6301 ggctcttgca cctcggcacg gtcataccca aggatgtcga ggtcgatcgt cctgggaccc
     6361 cagcgctcct ccctcacccg cccgagggcc gcctccacct ggtggcaggt acggagcagc
     6421 gctccgggat ccagctcggt ccggacctcg aggacggcgt tcaggtagtc gggttgccga
     6481 ggccccccca ccggcgcggt ctcgtacacg cgggacgatc tggccacccg caccaacggg
     6541 ctctcgtcca acagatccac cgccgcctgc aggttcgcca ggcgttcccc gaggttcgaa
     6601 cccagaccga tgaacgccgg gacctccacg cccggcccgc tcaaccggtg ggttccgccg
     6661 tggcggcggc cgccaccccc tggacgccgc tcgagatggc cgcgcccggc ttgtgcacga
     6721 tcgcggtggc acgcaccacg ccggagcggg cggcgacggc ctcggcgacc gcggtcgcga
     6781 tcgactcgag caggtcgaac gaggtcccct ggaccacctc ccgcgcggtc cggatcagcg
     6841 ttcggtagtc ggccgtcccc tccagcgtgt ccgaggagac ggcgacctcg acgtccaggt
     6901 ccaccacgaa ggcttgcggg cggtccttct caccggcgtt ggcaccgtgc cggccctcca
     6961 cgtcgatgcc ggtcaggaac agcctggacg tgctcacccc gcctccccct cgaagggctt
     7021 gcgctgcagc tccacgacca ccggtccgcc ccgatgctca tgcgagcgac ccaggtaccg
     7081 ggagcgaaga tactcgaaca tcagcggcgc gatcgtctcc ggcgcgtcct ccggcaggaa
     7141 gtgcgagcag ccggggagca ccgcgagtga ggcggtgggc accgcctcgg ccaggcgctc
     7201 cgcgatgtcc accggaaggt aggcgtcgtc ctcaccccac agcagcagga tcgggatgtc
     7261 gagccgtgcg agctcgccct cccggccggc caagccacgg ccgtcgaggg cccggaccag
     7321 tcggaggagc gctgcggcac cttcgggacc ctggaacggt cggaggtact cgcgcagcgt
     7381 ctcagccttg agccgctcgg gatgtcccat ccccgaacgc agccatcgcg ggaggaagcc
     7441 ctccgggagc ccctcagagg gctgccgccg ctgcacctcc cgcaccaaag gcgtgggcca
     7501 cgcgtcgaac gccaccgagt cgaccagcac catggcgcgc acccggccct ccagcgccag
     7561 cagttgggcg atccctccgc cggccccgtg gccgacggcc gcacacgatc cgatccccag
     7621 tgcctgcagc agctcgacca ggtacgcggt ctgagcccgg acgtgcaggg gtaggtccgg
     7681 gggcttgtcc gagtccccgg acccgaccag atcgggcgcg atcacgtgga accacggcgc
     7741 gaaggccggc acgaactcgc gccacaggta cgacgaggtg gggtacccgt gcagcaagac
     7801 gatggccggc tcctcgggat cgccctcctc cagataggag atccgcccga ccgacagctc
     7861 gacctgccgc ttctggagat cgctcatcgc ccaagcgcga tcgcatcgac cagacggatc
     7921 acgcgcgaga tctgcttcac gtcgtggacg cggacgatgc ttgcgccgtg cgtcgcggcc
     7981 caggcggcca cgccggcggt accctccacc cgctcgtcct ccgccagatc gagcagcgta
     8041 ccgatgaacc gcttccggga gggaccgacc aggatcggtc gtccgaggtc ccccagcgcg
     8101 tccagccgtc ggagcagctc aaggttgtgc tccaggtcct tcccgaaacc gatgcccggg
     8161 tcgaccacga gctgctctgg ggacaggccg gcgaagatcg ccgcctcgag gcgccgggcc
     8221 aggtagtcct tcacctcccc caccacgtcc gtgtaggtgg gggcctcctg catcgtcttc
     8281 ggatcacccc gcatgtgcat caacaccagc cccgctcccg cggccttcac cacgtcgaac
     8341 atgccgggct cccggccacc ggagatgtcg ttcacgatga cggcgccggc ccggagcgcc
     8401 gcctccgcca ccgccgcgtg gcgcgtgtcg atcgacaggg gcaccgcggt ccgcgaggcc
     8461 agagcctgca ccacgggcag gacccgccgc agctcttcct ccagcggcac gggatcggag
     8521 cccgggcggg tggattcacc tccgatgtcc accatgtcgg cgccgtcctt catcatcgcc
     8581 aggccgtgac ccagcgccgc gtcggggtcc aggaaccgac cgccgtcgga gaaggaatcg
     8641 ggcgtgacgt tcagtacgcc catcagcagc gtgcggccgc cgcactcgat ggtccgggcg
     8701 ccgcagcgcc agatcagggc cgtcccctcg ggcatgccac catcctagcg agggaccccc
     8761 cgacgaaccg gcggaggtct caggccttgc cgatgtggct gagggcctcc tggcgggtcc
     8821 gcgcatccgt gcggaacagc ccgcgaaccg cggacgtgac ggtccgggat ccgggcttct
     8881 tcaccccgcg catggtcatg cacaggtgct cggcttcgat catcacgaac acacctcggg
     8941 gttcgagcgc atcgtcgatc gcgtcggcga tctgggtggt gagacgttcc tggacctggg
     9001 ggcgcttggc cagcatgtcc acgacccgag ccaccttgga cagcccggtg atcctcccct
     9061 ccttgctggg cacgtaggcc acgtgggcct tgccgacgaa ggggatcaga tgatgctcgc
     9121 agctcgagta cagggggatg tccttcacca tgatcatctc gtcgaagtcc gccccgggga
     9181 tcacggtgac caacgaccgg gcgtccacac cgatcccggc gaagatctcc tcgtacatgt
     9241 cggccacgcg acggggcgtg tccttgaccc catcccggtc ggggtcgtcg ccgatcccgg
     9301 ccaggatcat ccggatgccc tcctcgatcc gctgccggtc gaagctcatg ggagccccac
     9361 tctacgcagg gagcggagcg cgggaaccgg cgcgacgccc ctggtcatca ggcgggcacc
     9421 agccggtcga gcgtgtccgg caggtcgtcc aggtcgggaa gctccgccgc tcgtgacagc
     9481 tgcgagctct ctcccaagcg gttgatccag atcgaccgca agcccatcgc ggcgcagggc
     9541 tcgatgtcgt ggaagagcga tgctgcgacg tgcgcgtgcc ggtcccggtc ggcaccggtc
     9601 cgctcgaaga aggtctccca gtgaccgggg gagggcttgt acgagcccgc ctccgccgcc
     9661 gtgatccgcg cgtccaccgg cacggagatc aaggacaggg acgcatccag cagatccgga
     9721 tccgtgttcg acaagatcgc gatgcgccac ccgcggtgcc gtagctcgtc cagcgcgggg
     9781 ggcacctcgg ggaacggcgg ccaggtgggc agcgactcgg ccagggcggc tcggtcgtcg
     9841 gcggcgagct cgagcccctc tcgatgcgcc agccgctcga gggtctcgga caggacctgt
     9901 cggtagctga tcgatcgccc gcgctgcacc tcgggctcga tctcgtggta ggcgccgagg
     9961 agaagctcag catcgtgttc cgggaacagc tcggacagcg tcatccggat cccgccaagc
    10021 cagtcgatga gcgttccgta gcaatcgaag gtcgcccagc gctcgctcat ggcggggtga
    10081 gtctagagga cgcggaccgg gatcaccggg gtcatcgaag cggcgccggg ccctgatccc
    10141 gctgcgcccg aaccgcccgg cgcgagaccg ccagcccggc cccgcggacg gcggcatccc
    10201 gctgggctcg cttcggcacc tcggccagga tccgctcgac ctcgtccttg tcgatggtct
    10261 cccgttccat gagggcggcg gcgatctcgt ccagcttcgc ccgccgctcc tgaaggatct
    10321 ccagcgcctc gtcgtgcgcc tgatcgatca tctggcggac ctcgttgtcg atctcgaagg
    10381 cgacctggtc cgagtagtcg ggctgggaag cgaagtcgcg ccccaggaag acctcgtgct
    10441 gcttctgtcc gagggtgagg ggcccgatcg tgtcgctcat cccgtactcg gtcaccatct
    10501 gccgagccac cttggtggcc cgctcgatgt cgttcgcggc gccggtggtg atatcgccca
    10561 cgatcagctc ctcggccacc ctcccgccga gcagcatggc cagctcgtcc gacagctcct
    10621 cgcgcgtcat gaggaacttg tcctcttggg gcagggtgag cgtgtatccc agggcccggc
    10681 cccgcgggat caccgagatc ttgtgcaccg ggtcggtgtt cggcagcacg tgggcgacca
    10741 gcgcgtgtcc cccctcgtgg taggcgatca tccgcttctc gcgctcgttc atgacccgcg
    10801 tcttgcgctc gggtccggcc atcacccggt cgatcgcctc ctcgacctcc ttcatgccga
    10861 tggccttcct gccccaccgg gcagccagga gggccgactc gttgatcacg ttggacaggt
    10921 ccgcgccggt gaacccgggg gtgcgacggg cgatcacgtc gagctcgacg cttccgtcga
    10981 acggcttgcc gcgcgcgtgg accttgagga tccccttgcg gccctccaga tccggacggt
    11041 cgatcgtcac gtgccggtcg aaccgtccgg gacgcagcag cgcagggtcg aggatgtcgg
    11101 ggcggttcgt ggccgccatc aggatcacgg ccgtccgctg gtcgaacccg tccatctcca
    11161 ccaggagctg gttcagggtc tgctcgcgct cgtcgtgccc gccgcccagt cctgcaccac
    11221 ggtggcgacc cacggcgtcg atctcgtcga tgaacacgat ggccggcgcg ttcgccttgg
    11281 cctggtcgaa cagatcccgt acccgggcgg cccccacccc gacgaacatc tccacgaagt
    11341 cggatccgct gatcgagtag aagggaaccc cggcctcacc ggccaccgcc cgagccagca
    11401 gagtcttgcc ggtaccgggc ggtccgaaca ggagcacgcc cttggggatc ttggccccca
    11461 tggcctggaa cttggccggg ttctgcaggt actccttgac ctcctgcagc tcctcgatgg
    11521 cctcgtcaac gccggccaca tcggtgaacg tggtcttcgg ctggtccttg ctgaccacct
    11581 tggcccgagc cttgccgaac tgcatcaccc ggccgccgcc gccctgggac tggttcatga
    11641 tccagatgaa cgctccgatg atgaggatca ccgggatcag ctggaccagg aagccaagga
    11701 gggccgagcc cttctgcgga tccacgttca cgtcgacgtt ctgggcccct agctccttga
    11761 tcagagactc ctggctcgct tccggatagg cgacctggta cgaggtgttg tcggtgagct
    11821 cgcctcggac ggtctgatcg ccttcgagga tcgtggcggt gcggaccttc ccctgatcca
    11881 cgaagctctg gtactggctg tacgagagct tccccccctg ggagccgtgg ttcgtgagca
    11941 ggacgaacat ccacaggccg aacagggcga tcagcacgta gagggccgga ccccggagga
    12001 tccggcgata cggagactcg ggcggggacg ccaggagggg ctcctttcgg atcggaggag
    12061 acgccgcagc cccgggacgc cctccgctcc gggaccacgc cgatggtagc aaggggggtt
    12121 ccgtgcgacc agacccccga ggaggaccgc tcagcggatc ccactgtcca cgggcacctt
    12181 gacctgggcg gatccgccct cagggacggt gacactcacg agatggccga tgcgcggccc
    12241 ggctccgtcc ggctcggctc gcacctggta ccggccgggc gggacctgca ccccgaaacg
    12301 gccgcgcgcg tcggtgacgg cggtggcgac cacctggccg cgatcgttcc tgacctccac
    12361 cgtggtgtcg gcaagaggcc tgggcggaca cgggctctcc gcgctcacga tggggcagac
    12421 cggaccgagc agcaccactc cccggatccc ggtggtcggc gtggtacccg tcccggactg
    12481 ggtcggagag gagctgcacg ctgccgtcag caccggcacg aggaggacca gcgcgatgcg
    12541 gcctcggttc atggcgcttg gacgggtgga tgggcagggg ccgttcccgt caccacccga
    12601 cgcggatcag agatcccggt aagcctcggg cttcagggtg gcgatgtacg gaaggttccg
    12661 gtacttctcg gcgaagtcca gaccgtagcc gatcacgaac tcggggggga tctcgaaccc
    12721 cacgtaccgc agatccaggt ggacctgctg gatgcccacc ttgtgcagca gcgcgcagac
    12781 ctccaggctg gcggggcggc ggccgcggag gttcttcagc aggtagttca gcgtgagccc
    12841 cgagtccacg atgtcctcga tcagcaggac gtgccgaccc tcgatgtcgt ggtccaggtc
    12901 cttcaggatc cgcaccacgc cggaggtctg cgtggacgcg ccgtaggagg ccacggccat
    12961 gaagtcgaag tccaccggca tccggatgtg acgggcaagg tccgccatga gcacgaacgc
    13021 ccccttcagg acgcccacga gcaggggatc ggtgccagcg tagtcatggg tgatcgctgc
    13081 ccccagctcc gccagcttgg cctggatctc ctcccggccg atcaacacct tctggatgtg
    13141 ccgctcgtac tcctcggcga acggcgcttc cagcggtgcc gacgaggtcc cgttcggttc
    13201 ggacagtgcg acccggcctt ccatctcgcg gccccctttc atccccggct ctcgggggaa
    13261 gagcgagaga gggaaacata ctccttgcgg cgcgcggcca ccaatccgcc cgtgaggtgc
    13321 cgtttccggc ccggccgccc ggccgagagg tccaggatgg cgtcgacgtc atcggcgctc
    13381 gggagcacgc cgcagcggta cacggccgag cgggccacgc ggctcccgat ggcccagggc
    13441 aggctcgtca gcgccagggc gtcgagccgt atcccctcgg ggacctcctc caggaactcg
    13501 accatggcct cggtggcccg gcgctcgagc tcctcggcgt ccctcctcaa cagggccgag
    13561 gtccgcgcga tgggcgcccg gacgtcgcgc cgggtggcgt cctcgatcgc cggaagagct
    13621 cggaggcgca cggcgttgcg gaacagccgc acatcgcggt tcgtgggatc gatccggggc
    13681 cgcagatgac gagcccggca gaaggcgttc acctcgtcgc gcgtcacatc gatcagggga
    13741 cgcacgtagg ggccgtgcac gggccgcagc ccggccaccg cgtccaggcc tccaccccgc
    13801 aggagggcga tgagcacggt ctccacctgg tcgtcgagcg tgtggccgat cgccgcccgg
    13861 ctcgccccgg cctcgcgcat cgtgagggcc agcgcacgca gccgaacccg gtgcgcccag
    13921 tcctccaccg aggcgccggc cggcggaacg gtctcggcca cccgcaggtg gaagggcagc
    13981 cgcaggcgcc gagcggcgcg ctccacgtac gcagcgtcat cggccgagcc ggggcgcagc
    14041 cggtggtcga ggtggaacac ctcgagccgg atcttcagca gccggcgaag ctcgaccagc
    14101 gagtgcagca ggcacatcga gtccggtcca cccgagaccg ccaccatcac cgtctcgccg
    14161 ggcaggaaca tcccgtgctc atggaccgtg gcccggaccc gatgcaggac ccgggccacg
    14221 gtcgggggcc gagccatcag ccgccggcga cgcgggccag ccagcgctcg ggctgagcga
    14281 tctcctggaa ggtgggaagg ttcgcctcgt cgagccagac gaggttcagc gcccgcatcc
    14341 cgccccgacc gtggaccgcc cgcacgaagg cctctccgga cgagtactga cggatcttcg
    14401 agtcgaaccc gatggcgcgc tggaacgtct tctccatacc cgaaacgctc cgtcgctcct
    14461 tcaggctccg acgcagccgc gggagatcgc ggacgtggtc ccgggccacc tcgtccatca
    14521 cgaagctggc gtgcccctcg agcaacgaca tcatcgcctg catgcgatgg aagaggtcgc
    14581 gttgctcggg ggtcatgagg aacatcagca ggttggcgct ccgccactcg gtgctgacgc
    14641 gggcctcggc cacggcgcga cggagctgag ccagcatctc gcgggcgtcc agctgcacgg
    14701 tgtcgaggta cgtgttcacc atccggttca ggtatcccct cagccacggc gtcgcgccga
    14761 actggacccg gtgggtcacc tcgtggatcg ccacccacat ccggaagtca tgggcgggca
    14821 gcgcgaaccg acgttcggtc tcggccaggt ttgggccgac gaagtagagc aggccctcgt
    14881 cgtcgggcgg gaggaagacg tcgtactgac cgagcacctt cttggacacg tagcccagga
    14941 gcacgccgat ctgcgctccc aacgccttgc gccgaaggtc cgagcgccgg gcgttctccg
    15001 cgagcatccg ccgggccagg ggctcgagca gccgctggag cccccgcagg ttcgtggcca
    15061 cccactcgcc gcgcgacatc acccacgcgc gggagcggaa gccgccggcg gccagacccg
    15121 tgaagccgga gaccagttcc tccgcgtggg gcacggtcgc ggacagatct tcccggagtc
    15181 gagcgcgctc gatcgcctgc aagaccggac cgcgaccacc gacccgacgg ccgacgttgg
    15241 cagcgacccc ccagtcgacc agcttggccg cctgagagcc catggaccaa tcgacc
//
LOCUS       DASQQQ010000091         4207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_200308, whole genome shotgun sequence.
ACCESSION   DASQQQ010000091 DASQQQ010000000
VERSION     DASQQQ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200308"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1556)
                     /locus_tag="VE646_03830"
     CDS             complement(186..1556)
                     /locus_tag="VE646_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZD79156.1"
                     /translation="MSADVVEIDLQLVERLTKEETDALDARHRRSLEYREVAKSHLPG
                     GVSSSWQSWPPHPIYVSHGQGSHVWDIDGNEYVDYHNGYGVMVMGHAHPRVVEAVQRR
                     VELGTHFAQPTEDALVCAENLAERFGLPLWRFGNSGTEATLDACRIMRAVTGRKLLVK
                     VEGTYHGHHDSLMVSVFPSQEDAGPRDHPASRPQTLGMPDGIVEQVICVPFNDASAVR
                     RAFAEHPGTIAGMIVEPAMSNCGLVLPDAGYLQALKDICHANGALLAFDEVKTGCTLA
                     WGGAVEAFGVVPDLVCLAKAIGAGLPCGAIGGSEEAMGMIVRGELEQVGTFNGNPLTM
                     AAMKVNLTEVLTKDAYREFDRIDGVLARCGEVIDRYRLPASIKVLGAKGSIDWHHEPI
                     HEYRDLWQIDDRIPQLAWLWQLNRGVFKSPGSKWESWTSSIVHTDEDVRRYVDNFEAL
                     AEAITA"
     gene            complement(1574..2467)
                     /locus_tag="VE646_03835"
     CDS             complement(1574..2467)
                     /locus_tag="VE646_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZD79157.1"
                     /translation="MSGEPECFVVRLADGRSLEALTAGPAEGDVFILHHGSPGSAVAF
                     DPFTEACVARGLRFVTFSRAGFGDSSRRSGRSVASVAADVAELADHLGAERFHVAGWS
                     GGGPHALACAALLPERVIAAATIAGVAPWDADGLDWTAGMGEDNQVEYPLAAGHPDEL
                     LRWMQPHVDALSRATAEQVVEDLSSVLSAVDRAALSGAFGAYEAKMFHGAFRHGPWGW
                     YDDDLAFTRSWGFELGEIRVPVSIWQGPHDLMVPFAHGGWLAAHIPGARAHLEPEHGH
                     LSLALGRFGDILDDLVASGRE"
     gene            complement(2486..3868)
                     /locus_tag="VE646_03840"
     CDS             complement(2486..3868)
                     /locus_tag="VE646_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZD79158.1"
                     /translation="MAVRETIEFDLDLVDHLIEEEEAKLEPKHRASIAYREVAESTVA
                     GGVASSWQDSPPHAIYIDHGERNRLWDIDGNEYVDYHLGYGAMVVGHAHPKVVEAVER
                     QVRRGTHFAQPTKDLDVVGESLADRFRVPLWRFMNSGTEATLEATRLMRANTGRNVIL
                     KVEGTYHGHHDSLMFSVVPDPAKIGPREHPVTLPQAMGIPQAFADLVRVVPFNDLQEV
                     ERALAEHEGDVAGMIVEPAMMNCGVILPEPGYLQGLKDLMHGAGAYLAFDEVKTGATI
                     AYGGAVEAFGVTPDIVCLAKAIGGGLPCGAMGATEDLFRPVLEGEYDMAGTFNGNPLT
                     MAASRATLTEILTKDAYERFDQVHVGLAEGCTAVIEKYRLPAYVTGMGAKGSVIYSPT
                     PVREYRDTVDIDERITYLAWLFQQNRGVFKSPWSKQETWTTSVWHGDEDVQRYVANFE
                     AFAAAITGSA"
BASE COUNT          639 a         1515 c         1398 g          655 t
ORIGIN      
        1 gggggcctgg accacctcgt cctggggcac ggtctggatg ggaagctccc gcccaaggtc
       61 tcgaggcacg gtgtggatgg cggcccgcat gtccatcagt atcgaacatg tgtttggacc
      121 tggctgtgac ctaggtcaca gacgcagcgg accgtggtcc ttcccccgaa cgcgacggac
      181 cgctgctacg ccgtgatggc ctcggcgagc gcctcgaagt tgtcgacgta ccttcggacg
      241 tcctcgtccg tgtgcacgat cgagctggtc cagctctccc acttcgaccc ggggctcttg
      301 aacaccccgc ggttcagctg ccagagccac gccagctgtg ggatccggtc atcgatctgc
      361 cacagatccc ggtactcgtg gatcggctcg tggtgccaat cgatcgagcc cttggcgccg
      421 agcaccttga tcgaagcggg cagccggtac cgatcgatga cctccccgca ccgtgccagc
      481 acaccatcga tccggtcgaa ctcccggtag gcgtccttgg tcaggacctc ggtcaggttc
      541 accttcatcg cggccatcgt caacgggttg ccgttgaagg tgccgacctg ctcgagctca
      601 ccgcggacga tcatgcccat ggcctcctcg ctgccaccga tcgccccaca gggcaggccg
      661 gccccgatgg ccttcgccag gcacacgaga tcgggaacca cgccgaaggc ctccactgcg
      721 ccgccccagg cgagggtgca cccggtcttc acctcatcga aggccagcag agccccgttc
      781 gcgtggcaga tgtccttcag cgcctggagg taaccggcat ccggcagcac caggccgcag
      841 ttcgacatcg cgggctcgac gatcatgccg gcgatggtcc cggggtgctc cgcgaacgcg
      901 cggcgcacgg ccgaggcgtc gttgaagggc acgcagatca cctgctccac gatcccgtcg
      961 ggcatcccaa gggtctgcgg ccgcgaggcc gggtggtccc tcgggccggc gtcctcctgg
     1021 gaggggaaca cgctcaccat cagcgagtcg tggtgaccgt gataggtccc ctccaccttg
     1081 accagcagct tccggcccgt gaccgcgcgc atgatccggc aggcgtccag cgtcgcctcg
     1141 gtcccgctgt tgccgaaccg ccacagcggc agcccgaagc gttcggcgag gttctccgca
     1201 cagaccagag cgtcctccgt gggctgggcg aagtgggtgc cgagctcgac ccgccgctgc
     1261 acggcctcca ccaccctggg gtgggcgtgc cccatgacca tcacgccgta gccgttgtgg
     1321 tagtcgacgt actcgttccc gtcgatgtcc cacacgtgcg atccctggcc gtgcgagacg
     1381 tagatcgggt gcggcggcca ggactgccag gacgagctca ccccgccggg caggtgcgac
     1441 ttggccacct cgcgatactc gagggagcgc cggtgcctcg cgtcgagcgc gtccgtctcc
     1501 tccttcgtca ggcgctccac cagctgcagg tcgatctcca ccacgtccgc ggacatggct
     1561 cccgcctcct ccctcactcc cgtcccgacg ccaccaggtc gtcgaggatg tctccgaagc
     1621 gccccagcgc cagcgacagg tgaccgtgct cgggctccag gtgggcccgg gcgccgggga
     1681 tgtgcgccgc gagccacccg ccgtgcgcga acggcaccat caggtcgtgc ggtccctgcc
     1741 agatcgacac cgggacccgg atctcgccga gctcgaagcc ccaggaccgg gtgaacgcca
     1801 gatcgtcgtc gtaccacccc caggggccgt gccggaaggc cccatggaac atcttcgcct
     1861 cgtacgcccc aaatgcaccg ctcagggcgg cccgatccac ggccgagagg accgaactca
     1921 ggtcctcgac cacctgctca gcggtcgccc gcgatagcgc gtccacgtgc ggctgcatcc
     1981 accgcagcag ctcgtcgggg tggccagcgg cgagcgggta ctcgacctgg ttgtcctcgc
     2041 ccatgccggc cgtccagtcc agcccgtccg cgtcccacgg cgcgacaccg gcgatggtgg
     2101 ccgcggcgat cacgcgttcg ggcagcagcg cggcgcaggc cagggcgtgg gggccaccgc
     2161 ccgaccaccc ggccacgtgg aagcgctcgg cgccgaggtg gtcggccagc tcggcgacgt
     2221 cagcggcgac ggaggccacc gagcgccccg accgcctgga ggagtccccg aagccggcgc
     2281 gggagaaggt cacgaaccgg agcccgcgag ccacgcaggc ctcggtgaac gggtcgaacg
     2341 ccacggcgga tcccgggctg ccatgatgca ggatgaagac gtctccctcc gccggaccgg
     2401 cggtcagggc ctccagggac cggccatccg ccagccggac gacgaagcac tccggctcgc
     2461 cgctcatcgg ctggaggcta ccgcgtcagg ccgagcccgt gatggccgcc gcgaacgcct
     2521 cgaagttcgc cacgtaccgc tgcacgtcct cgtcgccatg ccagaccgag gtggtccagg
     2581 tctcttgctt cgaccacggc gacttgaaga ccccgcggtt ctgctggaac agccaggcca
     2641 ggtaggtgat ccgctcgtcg atgtccacgg tgtcccggta ctccctgacc ggcgtggggc
     2701 tgtagatcac cgagcccttc gctcccatcc cggtcacgta cgccggcagg cggtacttct
     2761 cgatcaccgc cgtgcacccc tcggcaagac ccacgtgcac ctggtcgaag cgctcgtagg
     2821 cgtccttggt caagatctcg gtcaacgtcg cgcgcgaggc ggccatcgtg agcgggttgc
     2881 cgttgaaggt gccggccatg tcgtattcgc cctcgagcac cggccggaac aggtcctcgg
     2941 tggcgcccat cgccccgcac ggcagccctc cgccgatcgc cttggccagg cagacgatgt
     3001 cgggggtcac gccgaaggcc tcgacggccc cgccgtaggc gatcgtggcc ccggtcttga
     3061 cctcgtcgaa cgcgaggtac gcgcccgcgc cgtgcatcag gtccttcagg ccttggaggt
     3121 agcccggctc gggcaggatc acgccgcagt tcatcatggc cggctccacg atcatgccgg
     3181 ccacgtcgcc ctcgtgctcg gccagcgccc gctcgacctc ctggaggtcg ttgaagggga
     3241 ccacgcggac cagatccgcg aaggcctgcg ggatacccat ggcctggggg agggtcaccg
     3301 ggtgctcgcg cggcccgatc ttcgcggggt cgggcaccac ggagaacatc agcgagtcgt
     3361 ggtgaccgtg gtaggtcccc tcgaccttca ggatcacgtt gcggccggtg ttggctcgca
     3421 tcagccgggt ggcctcgagc gtggcctcgg tccccgagtt catgaaccgc cacaacggca
     3481 cgcggaagcg atcggccagg ctctccccca ctacgtcgag gtccttggtg ggctgggcga
     3541 agtgggtgcc acgccgcacc tggcgctcga ccgcctcgac caccttggga tgggcgtgac
     3601 cgaccaccat ggccccgtat cccaggtggt agtcgacgta ctcgttcccg tcgatgtccc
     3661 acaggcggtt gcgctcgccg tggtcgatgt agatcgcgtg aggcggcgaa tcctgccagg
     3721 aggacgccac gccaccggcg acggtgctct cggccacctc ccggtacgcg atcgaggctc
     3781 gatgcttggg ttcgagcttg gcctcctcct cctcgatcag gtgatcgacg agatccaggt
     3841 cgaactcgat ggtctcccgt accgccatgg ttcccagcct ccctgggggt cgcgctccgg
     3901 ccgtttgaac ggccattcaa gattcacagc catcatacgc cctgtcaagg gcgcgagcct
     3961 tgggcgctat cggagaccgg cgcgcgagag cccggccccg tgcggggccg ggctctgctg
     4021 ggcatggcga gactctctag gaatcgcgag gtaataaggt tgcctccagc gtgacaggct
     4081 ctaccatgag cgcatggtgg gggaagaggc cgtcgggcgg agagtggcca cgctgtggcc
     4141 ccacctgaac gagcgtcagc gtcgcctgtt gctcgcagcg gaggcccggg agctgggccg
     4201 tggagga
//
LOCUS       DASQQQ010000092         4429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_33739, whole genome shotgun sequence.
ACCESSION   DASQQQ010000092 DASQQQ010000000
VERSION     DASQQQ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33739"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VE646_03845"
     CDS             <1..573
                     /locus_tag="VE646_03845"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZD79159.1"
                     /translation="ARRRQDLLALSQRQASQAQALSWARGALLDSVAGAQRTVTELTA
                     KLKKEQRVISLLGQHVNAGGAIAVCPVRGPNAFVDSFGWPRSGGRTHQGIDLIAPDGT
                     PVVSVQPGRAEAHPNTLGGNAVIVYGPGGDWTYYAHLSSYGQMGSVGAGAVIGYVGST
                     GDTNVDHLHFEYHPGGGGAVDPYQALLAVC"
     gene            650..1183
                     /locus_tag="VE646_03850"
     CDS             650..1183
                     /locus_tag="VE646_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aerial mycelium formation protein"
                     /protein_id="HZD79160.1"
                     /translation="MAVSNGNRRIDRILRPDFADAPSELSLDELRERRDECLAEREYL
                     SLLRRLVQGRAEILKAELERRGGAVMEDAPLVERLSKILAGEGHGAARGEAVRVGVPE
                     DEILHARRRVERMVADAGLSDPGSLDDRRLREAVEVLATEEHQVSESRAAVIGVLDRL
                     QEELKRRYKQDPSTVLD"
     gene            1375..2145
                     /locus_tag="VE646_03855"
     CDS             1375..2145
                     /locus_tag="VE646_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZD79161.1"
                     /translation="MTRSPSHDRQRSAAGFDPEFPTVQRQLLVFARELGELYHLERQR
                     SEDLERALQSLKETYLATMTSLAQVVEAKDLTTRGHLDRTRAFGVALARRIDPALAEG
                     PELDYGFFLHDIGKVGIPERILCKAGPLSADEWEVMRTHPTIGAQIVAPIKFLGSAVD
                     VIRHHHERFDGHGYPGGLRGDRIPLAARIFAVADSYDAMTSDRPYRDALRTDRALDEI
                     RLGAGKQFDPDVASVFIDLVEEDACPVPAAPQGVARSS"
     gene            2162..3139
                     /locus_tag="VE646_03860"
     CDS             2162..3139
                     /locus_tag="VE646_03860"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HZD79162.1"
                     /translation="MPAAVPLVRVIRSGLEESVHLGHVAVCDASGRLVARAGDPRRPV
                     FARSCMKPIQGAVSLGAIGEDLPDDLIAVMCASHNGEPVHVRAVRRLLRRAGLGASSL
                     RNPPGWPLDARSMARAGRPSRLLHNCSGKHAGMLFGCVRSGWDTERYRRRTHPLQRRV
                     LGTVVAGSGRSEVRIGVDGCGVPVHGMPLVAMATLYARFGQPDRFGAVSAAVARVVDA
                     MRAEPYLVAGRGRVDTALMRAAPGLLVKGGAEALHCAWIPEGGLGVAVKVLDGGERAA
                     GPTLIRTLRLLGALNQRQLERLGSFAERPVSGGGRPVGCVEAAFELRGR"
     gene            3178..>4429
                     /gene="mshA"
                     /locus_tag="VE646_03865"
     CDS             3178..>4429
                     /gene="mshA"
                     /locus_tag="VE646_03865"
                     /EC_number="2.4.1.250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol-3-phosphate glycosyltransferase"
                     /protein_id="HZD79163.1"
                     /translation="MALTAGVDGRPRVAVVSLHTSPLDQPGTGDSGGMNVYIRQAADR
                     LTACGVDVDVFTRRRDPRDQPVEELPGGSRIIRVKAGPPVPVPKQELPRFLPEFLGGV
                     LGRQRADGTRYDVVHSHYWLSGWVGRSTKEIWRVPLVASFHTLGKVKNYSLAQGEPPE
                     PPVRLSGEEAVIAEADRIMAATPVEAAQLVGLYRADPGRIRLAPPGVDHQLFFPRPRD
                     QARDRLHLTGVRLVLFVGRLQPHKGPDIAVRALAEAIARDPRGTRDVVLAVVGGPSGS
                     SHGAEVAHLMDLAGALGVGERVVFFPPQPQQRLADFYAAAECVLVPSRSESFGLVALE
                     AQACGTPVVAAAVGGLRYVVEDGATGYLVEGHDPADHAERLLQILADPTLGRRLGTSA
                     VARSLRFSWDATAVEILSVYRELLQ"
BASE COUNT          574 a         1519 c         1684 g          652 t
ORIGIN      
        1 gcccgccggc ggcaggacct gttggcgctg tcccagcgac aggcctcgca ggcgcaagcc
       61 ctgtcctggg cccggggtgc gttgctcgac tccgtggccg gggcccagcg cacggtgacc
      121 gagctcacgg ccaagctcaa gaaggagcag cgggtgatca gcctcctcgg ccagcacgtg
      181 aacgccggag gcgccatcgc cgtgtgtccg gtccgagggc cgaacgcctt cgtcgactcc
      241 ttcggctggc cgcgttccgg cgggaggacc caccagggga tcgacctcat cgcccccgac
      301 gggacgccgg tggtgtcggt gcagcccggc cgggccgagg cgcaccccaa cacgctgggc
      361 ggcaacgccg tcatcgtgta cggccccggg ggcgactgga cctactacgc ccacctgtcc
      421 tcctacggcc agatgggctc ggtgggggcc ggcgcggtga tcggctacgt aggctccacc
      481 ggggacacca acgtggacca tctgcacttc gagtaccacc caggcggcgg gggcgccgtc
      541 gacccctatc aggcgctgct ggccgtgtgc tgaggggctg ctcgccctga ggcccgtcca
      601 cgcgccggcg cgctacgatg gcgcgaaccc gggtgcgaag gagcgtgtcg tggctgtctc
      661 gaacgggaac cggcggatcg atcgcatcct gcgtccggac ttcgcggacg cgcccagcga
      721 gctctccctg gatgagctcc gagagcgccg cgatgagtgc ctcgccgagc gcgagtacct
      781 ctccttgctc cgccgactgg tccaggggcg ggccgagatc ctgaaggcgg agctcgagcg
      841 ccgcgggggt gcggtgatgg aggatgcacc tctggtggag cgtctgtcga agatcctcgc
      901 cggcgagggg cacggagcgg cgcgcggtga ggccgtccgc gtgggcgtgc cggaggacga
      961 gatcctgcac gcccgtcggc gcgtcgagcg gatggtggcc gacgcgggac tgtccgatcc
     1021 gggctccctg gacgaccgcc ggctccgcga ggcggtggag gtcttggcta cggaggagca
     1081 tcaggtctcg gagagccgag cagcggtgat cggggtcctg gaccggctgc aggaggagct
     1141 gaagcgccgc tacaagcagg atccgtccac ggtcctggac tgaccccggg cggaccgagc
     1201 tgacgcgtcc tgcgtacccc gggcggggga ccggcggtcc cgctcatcgt ccgaaggttc
     1261 atgggagccg ggggatggcc gtttgggcca acgtgcctat ggacccgcat gggtcgaaac
     1321 atcaggctgg gttccgcccc cggggcaccc cggtcgggta tcgtgcggcc ccagatgacg
     1381 agatcacctt cccacgaccg gcagcgatcc gcggcggggt tcgaccccga gttcccaacc
     1441 gtgcagcgac agctgctggt gttcgcccgc gagctcggtg agctctacca cttagagcgc
     1501 cagcggagcg aggacctgga gcgggcgctg cagagcctca aggagaccta cctggcgacc
     1561 atgaccagcc tggcgcaggt ggtcgaggcc aaggacctca ccacgcgtgg tcacctggac
     1621 cggacccggg ccttcggcgt ggcgctggcc cgacggatcg acccggccct ggccgaaggg
     1681 ccggagctcg actacggctt cttcctccac gacatcggca aggtggggat ccccgagcgg
     1741 atcctgtgca aggcgggccc cctctcggcg gatgagtggg aagtgatgcg cacccacccc
     1801 acgatcgggg cacagatcgt ggcccccatc aagttcctgg gcagcgcggt cgacgtgatc
     1861 cgtcatcacc acgagcggtt cgacggccac ggctaccccg gggggctgcg gggagaccgg
     1921 atcccgctgg ccgcccggat cttcgcggtg gccgactcgt acgacgccat gaccagcgac
     1981 cggccttacc gggacgccct gcggacggac cgtgcgctcg acgagatccg actgggggcc
     2041 gggaagcagt tcgacccgga tgtcgcatcc gtcttcatcg atctggtcga ggaggacgcc
     2101 tgcccggtgc cggctgcgcc acagggggtc gcccggtcct cctgagcgta ggctgaggcc
     2161 gatgccggcc gcggtccccc tggtgcgtgt gatccgctcg ggactggagg agtccgttca
     2221 ccttggccac gtggccgtgt gcgacgcgtc cgggcggctg gtggctcggg ccggcgaccc
     2281 acggcggccg gtcttcgctc gatcctgcat gaagccgatc cagggcgcgg tctccttggg
     2341 ggcgatcggt gaggacctcc ccgatgacct gatcgcggtg atgtgcgcat cgcacaacgg
     2401 tgagcccgta cacgtccgcg cggtccgccg gttgctgcga cgggccgggc tgggggcgtc
     2461 cagcctgcgc aacccgcccg gctggccgtt ggacgcccgg tcgatggctc gggccggtcg
     2521 cccctcccgc ctgctgcaca actgttcggg caagcacgcc gggatgctct tcggatgcgt
     2581 gcgttcgggc tgggacaccg agcgctaccg gcgtcgaacc cacccgctcc agcggcgggt
     2641 cctggggacc gtggtggcgg gaagcggtcg ctccgaggtc cggatcggtg tggacggctg
     2701 cggcgtgccg gtgcacggga tgccgctggt cgccatggcc acgctgtacg cgcggttcgg
     2761 gcagccggat cggttcgggg cggtgtccgc ggccgtggcc cgggtcgtcg acgcgatgcg
     2821 cgccgagccc tacctggtgg cgggccgggg ccgtgtcgac accgcactga tgcgggcggc
     2881 cccgggactg ctggtcaagg gcggggcgga ggcgctgcac tgcgcctgga tcccggaggg
     2941 tgggctcggc gtggcggtga aggtgctcga cgggggggag cgggccgccg ggccgacgct
     3001 gatccggacg ctgcgcctgc tcggcgcgct gaaccagcgg cagctcgaga ggctcggatc
     3061 gttcgcggag cgcccggtga gcggcggggg gcgacccgtg gggtgcgtgg aggcggcgtt
     3121 cgagctccgc ggccggtagg acccgatctt ccgcggaacc tcgcgtacca tcgttccatg
     3181 gctctcaccg ccggtgtgga cggccggccg cgcgtggcgg tcgtcagcct gcatacctcc
     3241 cccctggacc agcccggcac cggggactcc ggcggcatga acgtctacat ccgccaggcg
     3301 gcggaccggc tcacggcctg cggggtggat gtggacgtgt tcacgcggcg tcgggatccg
     3361 cgggaccagc cggtggagga gctgcccggt ggctcccgca tcatccgggt gaaggccggg
     3421 cccccggtgc ccgtcccgaa gcaggagctc ccgaggttcc tgccggagtt cctgggcggc
     3481 gtgttgggac gtcagcgggc cgatggcacg cggtacgacg tcgtgcacag ccactactgg
     3541 ctctcgggat gggtgggcag gagcacgaag gagatctggc gggttcctct ggtcgcctcg
     3601 ttccacacac tgggcaaggt caagaactac tcgttggcgc agggtgagcc cccggagccc
     3661 ccggtccgcc tctccggcga ggaagcggtg atcgccgagg ccgaccggat catggccgcg
     3721 acgccggtgg aggccgcgca gctggtcggt ctgtaccgcg cggaccctgg gcggatccga
     3781 ttggcgcccc ccggcgtcga ccaccaactc ttcttccccc ggccgcggga ccaggcccgc
     3841 gaccggttgc acctgaccgg ggtccggttg gtgctgttcg tgggccggct ccaaccgcac
     3901 aaggggcccg acatcgccgt gcgcgccctg gccgaagcga tcgcgcgcga tccccgagga
     3961 accagggacg tcgtcctggc ggtggtcggc ggtcccagtg ggtcctccca cggggccgag
     4021 gtggcgcacc tcatggacct ggccggcgcg ctgggcgtgg gcgagcgtgt cgtcttcttc
     4081 ccgccccagc cccagcagcg gctggcggac ttctacgccg cggccgagtg cgtgctcgtg
     4141 ccgtcgcgat ccgagtcctt cggcctggtc gccctggagg cgcaagcctg cggcacgccg
     4201 gtggtggcgg ccgcggtcgg ggggctccgg tacgtggtgg aggacggcgc gaccgggtac
     4261 ctggtcgagg ggcacgatcc cgccgaccac gcggaacgtc tgctgcagat cctcgccgat
     4321 ccgaccctgg gtcggcgcct ggggacctcg gccgtggcac ggtcccttcg gttctcgtgg
     4381 gacgcgacgg cggttgagat cctctccgtg taccgcgagc tccttcagg
//
LOCUS       DASQQQ010000093         8674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_337784, whole genome shotgun sequence.
ACCESSION   DASQQQ010000093 DASQQQ010000000
VERSION     DASQQQ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8674
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337784"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1301)
                     /locus_tag="VE646_03870"
     CDS             complement(192..1301)
                     /locus_tag="VE646_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD79164.1"
                     /translation="MGGGEVRLVDLVKRFADVTAVAGINLQMPPGEFFSLLGPSGCGK
                     TTTLRLIAGFERPDEGQILLDGVDMANVPPHKRNVNTVFQNYALFPHLNVFENVAFGL
                     RYKDVSKQDTRRRVLESLSLVRMEGLEKRRPTQLSGGQQQRVALARALVLNPAVLLLD
                     EPLGALDAKLRKALQIELKALQEEVGITFVYVTHDQEEALTMSDRLAVMSNGRVEQIG
                     TPSEVYEEPATAYVADFLGVSNLMDATADGAAANGRCRVRIGDFELYAEQGEVDARGQ
                     TKVTIRPERIRLEAHGTSGENRVPGMVERVVYVGSIMQVIVHLAEGDTLQAWVQNQGQ
                     GSPFEQGSPVTVHMPAEALRVLQDTGAEVPATVDG"
     gene            1340..2584
                     /locus_tag="VE646_03875"
     CDS             1340..2584
                     /locus_tag="VE646_03875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD79165.1"
                     /translation="MAVQDNPEDGLRPSHPGSTPAERGFGLAGQVAPLFAAYLAFGAF
                     WGVWVVVFADFLQARGMSEGAAGLYLSALAAVSILTMTLLAPRLHRLALSRTVPLGLG
                     SMGLAALGIAFGPGPGGLAGAFVFLGVGNGLIDVFVNVGGQGAEARHGRPVLQFLHAS
                     YNAGGIVGALGAAAMHVAGLSFRWQLTVTAGVLFAAAIWNVASRRLPDRAVSTAPDEE
                     RPMVSLSVFLRSPMLILPAVVVLSAFMVEGSMDIWSVIYLRRSLEATVVAGALAFAAF
                     SLAMAVGRMLAGRLLFGLGYRTTMRVSGAGSVASGLIAALTHSATVAGVAFLFLGLFI
                     ASAAPAAFGVIGETDEDPVLAVAGLTTAGYSGFVVGPPIMGWLAETAGLRATMLVVVV
                     AAVGVGAGGLGRRPRPAVPPSR"
     gene            complement(2592..4043)
                     /locus_tag="VE646_03880"
     CDS             complement(2592..4043)
                     /locus_tag="VE646_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZD79166.1"
                     /translation="MADALQNFIDGRWVDARDGERFDVFDPATGEVIATAPHSKQADV
                     EAAVQAARRTFDEGTWWPGTPARERGRILLRAADIVRREHENLAQLEARDSGKPIGEA
                     REDIEEVAFMFEYFGGWATKIEGDVHQLSSDAMFMVWKEPVGVAVGITPWNYPMTMAV
                     QKVAPAIAAGCTFILKPPEQTPLTCLQLPAILGEAGLPDGVFHVITGFGETAGAPLVA
                     HPKVDKIGFTGSREVGKIIMRSGADTLKRVTLELGGKSPNIVFPDADFDAAVEGSCNG
                     IFWNQGEICSAGSRVFVDGTIYDDALNAFVDRARQVKLGEPFDEGTTMGPLVSREQME
                     RVSRYIEIGGGEARLAIQGDPPADPKLSGGYFVPPTIFADVDNGATIAREEIFGPVMS
                     VIRFTDVDEVIRLANENDYGLAAAVWTNDLRKALTTARALRAGIVWINDTQPAPTEMP
                     WGGYKQSGIGRELGREGVDDYLERKSVFVNLAE"
     gene            4141..5001
                     /locus_tag="VE646_03885"
     CDS             4141..5001
                     /locus_tag="VE646_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZD79167.1"
                     /translation="MERERFVEAAGVRLAIREWPGQGPAVLLLHGLASTSHIWDLVAP
                     SLSRRHRVVAYDQRGHGRSGKPSTGYGFDRVSADAVAVIRGLRMRRPVVVGHSWGAAV
                     ALRVAHRTPQRVSGAILLDGGFSNLRDRMDWPTAKRLLAPPDIDGMPLERFLAWPRRA
                     LAGLIEVTPQVEEVFRSLVRVDRRGRIHRRLAVSNHLRILHAMWAQDTLAALRTVPVP
                     TLVLACRTAEPTGGEAGFHAAKVEAERAVRAIGEPVRFAWIEGIHDVPLQHPQLVARR
                     IERFVRRVVG"
     gene            5006..5203
                     /locus_tag="VE646_03890"
     CDS             5006..5203
                     /locus_tag="VE646_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79168.1"
                     /translation="MVTFILILLLLAAGFGVLGIVLKATLVIVLSVVLTVALLTAGVS
                     YWLRYRLYRFRRELERRGWGG"
     gene            complement(5225..5629)
                     /locus_tag="VE646_03895"
     CDS             complement(5225..5629)
                     /locus_tag="VE646_03895"
                     /inference="COORDINATES: protein motif:HMM:NF022700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 family protein"
                     /protein_id="HZD79169.1"
                     /translation="MRIGSDPSIGRRSRLLRPAVDAAALILFVLVGVVNHDGRITVGS
                     VARTAVPLLVAWFAVGWAAGAYRRPGPRTLLLTWLAATPLAVLMRSVLARGPWGMELL
                     EFLGVALAFTLLFVLVARLIAAAVLWFVPETG"
     gene            5753..6871
                     /locus_tag="VE646_03900"
     CDS             5753..6871
                     /locus_tag="VE646_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZD79170.1"
                     /translation="MSGLSRHARTRPGLRVGVRAAVVLGMATLPLLLGVSLHDLPPPV
                     RVVVNGEPVSLSDGTTFGGAIRMMHLRPRPGRLLDVQGGVLEARADPGRILLNGDVAP
                     RSRGLRSGDRISVVNGTDRTEGTRRVVDRLPGRHPGDPQYSLATSRMEQITVVGRRSG
                     KVVSVRYRSIGRSHAPPAVALTFDDGPWPKGTRGVLTVLERMHAKATFFMIGYLVDRY
                     PAIARDVARAGMAVGNHSWDHVEDPPFAKLKPHRLHTELAQANQAIGRATGVHPTLFR
                     PPAGSWDPEVLTVANRLGMRIALWDVDPRDWAASATPRSIAANVLRNVRPGAVVDLHD
                     GGGDAKATIRALPKIIRGIRKMGLQLVTMDQQLPPGPR"
     gene            6908..7336
                     /locus_tag="VE646_03905"
     CDS             6908..7336
                     /locus_tag="VE646_03905"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HZD79171.1"
                     /translation="MERATVLFDLECGFCRWTADRLAAWDRRGRLRFVALQEPEADRL
                     LPGMPEERKMGSWHLVLPNGRVRSAGDAVAPLMRRLPGGGPIAALATALPGPTERLYA
                     FVSRHRDGLAQVLGPRRSCVDPRSARRPGDRPNGKRARAR"
     gene            complement(7323..8234)
                     /locus_tag="VE646_03910"
     CDS             complement(7323..8234)
                     /locus_tag="VE646_03910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZD79172.1"
                     /translation="MPADRRALRGALGRTGVWSFTLETRSAAEERTAAERIEAMGYRV
                     LWFPEAVDSKEAFAQSALLLGATERAVVGTGIANIWARDPVAMANGWRALSDAHPGRF
                     LLGIGVSHRYAVDRRGHRYERPYSRMREYLDAMDAAPTSAPQPAGPLWRVLAALGPRM
                     LELAAERAEGAHPYFVPVEHTAFARARLGPDPVLAVEQTAVLQTDPSRARGIARTFAD
                     GYLASPNYARNLKRMGWGDHDIAGGGSDRLIDAVVAWGDVDRIVARVREHLEAGADHV
                     CLQVVAEAEDDVCLGQLEELAPALSEL"
     gene            complement(8246..>8674)
                     /locus_tag="VE646_03915"
     CDS             complement(8246..>8674)
                     /locus_tag="VE646_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZD79173.1"
                     /translation="FGLSMDYHVFLLTRIREEYDRIGHNTEAVAYGLRTTAGIITGAA
                     LIMVAVFTGFAAGRIGALQQMGFGLAVAVFMDATIVRTLLVPAGMRLLGDLNWYLPRW
                     LRWLPQIDVEGREPTQRVLQPALEPVGVAPAAEPVPRDDS"
BASE COUNT         1173 a         3192 c         2989 g         1320 t
ORIGIN      
        1 ccatgagcac gatgcgggct cggaacacgt ctcgatacgc cgacgtatac ttccgggccc
       61 gcttctccag gatggtctgc gtggacgcgt cgagaacgat ctcgtacggg ctcttcctcg
      121 gcacaggctc cctcctctcg ggggaaaccc taccgtacaa cttacgtcgg tgtaattatg
      181 gtggcgacca cttagccgtc gaccgtggcc gggacctcgg cgccggtgtc ctgcaggacc
      241 cggagcgcct cggccggcat gtggaccgtc accgggctgc cctgctcgaa gggggagccc
      301 tgaccctggt tctggaccca ggcctgcaag gtgtcgccct cggcgaggtg cacgatcacc
      361 tgcatgatcg agcccacgta caccacccgc tccaccatgc cggggacccg gttctcgccc
      421 gaggtgccgt gcgcctccag gcggatccgc tccgggcgga tcgtgacctt ggtctgtcct
      481 cgcgcgtcga cctctccctg ctccgcgtag agctcgaagt cgccgatccg gacgcggcag
      541 cggccgttgg cagccgcgcc gtcggcggtg gcgtccatga ggttggagac ccccaggaag
      601 tcggccacgt aggcggtggc gggctcctcg tagacctcgc tcggcgtccc gatctgctcc
      661 acccggccgt tgctcatcac ggccaggcgg tccgacatcg tgagggcctc ctcctggtcg
      721 tgggtcacgt acacgaaggt gatgcccacc tcctcctgca gcgccttgag ctcgatctgc
      781 agggccttgc gcagcttggc gtccagggca cccaggggct catccagcag gagcacggcc
      841 gggttcagga ccagggcccg cgccagggcc acccgctgct gctgcccacc cgagagctgg
      901 gtgggccggc gcttctccag gccctccatc cggaccaggg acagcgactc gagcacccgg
      961 cgcctcgtgt cctgcttcga gacgtccttg taccgcagcc cgaaggccac gttctcgaag
     1021 acgttcaggt gcgggaacag cgcgtagttc tggaagaccg tgttcacgtt gcgcttgtgg
     1081 ggggggacgt tggccatgtc cacgccgtcc agcaggatct gaccctcgtc cggccgctcg
     1141 aagcccgcga tcaggcgcag cgtggtggtc ttgccgcagc ccgaaggccc cagcaacgag
     1201 aagaactcgc ccgggggcat ctgcaggttg atcccggcga ccgcggtcac gtccgcgaac
     1261 cgcttcacca ggtcgaccag tcgaacctct ccaccaccca tggcaggggg gaggatacac
     1321 gactaccctg gctctttgca tggccgttca agacaacccg gaggacggct tgcgccccag
     1381 ccatcccgga tccacgccgg ccgagcgggg cttcgggctg gcgggccagg tggcgccgct
     1441 gttcgccgcc tacctcgcct tcggagcctt ctggggggtc tgggtggtgg tgttcgcgga
     1501 cttcctccag gctcgaggga tgagcgaggg ggccgcgggg ctgtacctct cggcgctggc
     1561 ggccgtgtcg atcctcacca tgacccttct cgctccccgg ctgcatcggc tggccctgtc
     1621 caggaccgtg ccgctcggcc tggggagcat gggcctggcc gccctgggca tcgcgttcgg
     1681 gccgggaccg ggagggctgg ccggcgcatt cgtgttcctg ggggtcggca acggtctgat
     1741 cgacgtgttc gtgaacgttg ggggccaggg tgcggaggcc cgtcacggcc gcccggtcct
     1801 gcagttcctg cacgcctcgt acaacgccgg cgggatcgtg ggggcgctgg gagcggcggc
     1861 catgcatgtg gccggcctct cgttccggtg gcagctgacg gtcaccgccg gggtgctctt
     1921 cgccgccgcg atctggaacg tcgcctcccg caggctcccc gatcgtgcgg tctccaccgc
     1981 cccggacgag gagcggccga tggtctccct ctcggtgttc ctgcgctccc cgatgctgat
     2041 cctgccggcc gtcgtggtgc tctccgcctt catggtggag gggtccatgg acatctggtc
     2101 ggtgatctac ctccggagga gcctggaggc cacggtggtg gccggcgcgc tcgccttcgc
     2161 cgcgttcagc ctggccatgg ccgtggggcg gatgctggcc ggccgcctgc tgttcggtct
     2221 ggggtaccga accaccatgc gggtgtccgg agccggctcg gtcgcctccg gcctgatcgc
     2281 ggccctcacc cacagcgcga cggtggccgg ggtggcgttc ctgttcctgg ggctgttcat
     2341 cgcctccgcc gccccggcgg ccttcggcgt gatcggcgag accgacgagg atccggtcct
     2401 ggcggtggcc gggctcacca ccgccggata ctccgggttc gtggtgggcc cgcccatcat
     2461 gggatggctg gccgagaccg ccggtctgcg ggccacgatg ctggtggtcg tcgtcgcggc
     2521 ggtcggggtg ggcgccggcg gcctcggccg ccgtccccga ccggccgtcc ccccgagcag
     2581 gtgaacgcgg gctactcggc caggttcacg aacacggact tgcgctccag gtagtcgtcc
     2641 acgccctccc gaccgagctc ccggccgatg ccggactgct tgtagccacc ccacggcatc
     2701 tcggtggggg ccggctgggt gtcgttgatc cacacgatcc ccgcccggag cgcccgggcc
     2761 gtggtgaggg cctttcgcag gtcgttcgtc cacaccgcgg cggccaggcc gtagtcgttc
     2821 tcgttcgcca gccggatcac ctcgtccacg tcggtgaacc ggatcaccga catcaccggg
     2881 ccgaagatct cctcccgggc gatcgtggct ccgttgtcga cgtcggcgaa gatcgtgggc
     2941 ggcacgaagt agccccccga cagcttgggg tcggccgggg gatccccctg gatcgccagc
     3001 cgcgcctccc ccccgccgat ctcgatgtac cgggagaccc gctccatctg ctcgcgcgag
     3061 acgagcggcc ccatggtggt gccctcgtcg aatggctccc cgagcttcac ctgcctggcg
     3121 cggtcgacga acgcgttcag cgcgtcgtcg tagatcgtcc cgtccacgaa cacccgcgac
     3181 ccggccgagc agatctcacc ctggttccag aagatcccgt tgcagctgcc ctccaccgcc
     3241 gcgtcgaagt ccgcgtcggg gaagacgatg ttgggagact tgcccccgag ctcgagcgtg
     3301 acccgcttca gcgtgtcggc cccgctgcgc atgatgatct tccccacctc gcgcgagccc
     3361 gtgaagccga tcttgtctac cttcggatgg gccaccagcg gcgctcccgc cgtctcgccg
     3421 aacccggtga tcacgtggaa cacgccgtcg ggcagccccg cctcgcccag gatcgcgggc
     3481 agctgcaggc aggtgagcgg ggtctgctcc gggggcttga ggatgaacgt gcaccccgcg
     3541 gcgatggccg gtgccacctt ctgcaccgcc atcgtcatcg ggtagttcca gggcgtgatg
     3601 ccgaccgcca cccccacggg ctccttccac accatgaaca tcgcgtccga cgacagctgg
     3661 tggacgtcgc cctcgatctt cgtggcccat cccccgaagt actcgaacat gaaggcgact
     3721 tcctcgatgt cctcccgcgc ctccccgatc ggcttgccag agtccctggc ctcgagctgg
     3781 gccaggttct cgtgctcccg gcggacgatg tcggcggccc ggaggaggat ccgcccgcgc
     3841 tcccgtgccg gggtccccgg ccaccacgtg ccctcgtcga aggtccgtcg ggcggcctgc
     3901 accgccgcct ccacatcggc ctgcttcgaa tgcggtgcgg tggcgatcac ctcaccggtg
     3961 gccgggtcga agacgtcaaa ccgctcccca tcccgagcgt cgacccacct gccgtcgatg
     4021 aagttctgca acgcatcagc cactacgatc cctccccgaa ggtgttgaac cgtcattcag
     4081 cataccgagg accgccggat cgggggaacc cagagcggcg tcccggtagg cttcggcccg
     4141 gtggaacgcg agcggttcgt ggaggcagcg ggcgtccggc tggcgatccg tgagtggccc
     4201 gggcagggtc ccgccgtgct cctgctccac ggcctcgcct ccacctccca catctgggac
     4261 ctggtggcgc ccagcctgtc ccggcgccac cgggtggtgg cctacgacca gcgcggccac
     4321 gggcgctcgg gcaaaccctc gaccggctac gggttcgacc gggtgagcgc cgacgccgtg
     4381 gcggtgatcc gcgggctccg gatgcgccgg ccggtggtgg tcggccactc ctggggggcc
     4441 gccgtggccc tgcgggtggc ccatcggacg ccccaacggg tctccggagc gatcctgctg
     4501 gacggagggt tctccaacct ccgcgatcgg atggactggc ccaccgccaa gcgcctgctg
     4561 gcacccccgg acatcgacgg gatgccgctc gagcggttcc tggcctggcc gcgccgcgcc
     4621 ttggccggcc tgatcgaggt caccccgcag gtcgaagagg tcttccgctc gctggtccgg
     4681 gtggaccggc ggggacggat ccaccgccgc ctggcggtct ccaaccacct caggatcctc
     4741 cacgccatgt gggcccagga cacgctggcc gccctgcgga ccgtgccggt gcccacgctg
     4801 gtgctggcct gcaggaccgc cgagcccacc ggcggcgagg ccgggttcca cgctgccaag
     4861 gtcgaggccg agcgtgcggt ccgggccatc ggcgaaccgg tccgcttcgc ctggatcgag
     4921 gggatccacg atgtcccgct gcagcatccg cagttggtgg cccggcggat cgaacgattc
     4981 gtgcgacggg tcgtaggatg agcacgtggt cacgttcatc ctgatcctgc tgctgctggc
     5041 cgccgggttc ggcgtgctcg gcatcgtcct gaaggcgacg ctggtcatcg tcctgtcggt
     5101 ggtgctcacg gtcgcgctgc tcacggccgg cgtctcctac tggctgcgct accgcttgta
     5161 ccgcttccgg cgcgagctcg aacgacgcgg ctggggcggg tgaccgagcc cttcggatcg
     5221 gacgctatcc ggtctcgggg acgaaccaga gcaccgcggc ggcgatcagg cgggccacga
     5281 gcacgaagag caacgtgaag gccagtgcca cccccaggaa ctcgagcagc tccatccccc
     5341 agggcccgcg cgccaggacc gagcgcatca ggacggccag gggcgtcgcc gcgagccagg
     5401 tgagcaggag cgtacgtggt ccgggacgcc ggtaggcccc cgccgcccac cccaccgcga
     5461 accaggccac gagcagcgga acggccgtgc gggccaccga tcctacggtg atccgaccgt
     5521 cgtggttcac gaccccgacc aggacgaaga ggatcaatgc cgccgcgtcc accgccgggc
     5581 gaagcagccg cgagcggcga ccgatggagg gatcgcttcc tatccgcacc ccctcatcct
     5641 cccagaccgg cggtccgccc cgggtcggat cgttgagcct gggggggttc cgtggttcga
     5701 taccttctca ccccatggcc gaggtgaacg tcccaccgat gtcgagggca acgtgtccgg
     5761 actgagtcgg cacgcccgga cccggcccgg gttgcgggtc ggggtgcgcg cggccgtcgt
     5821 gctgggcatg gccaccctgc cgctcctgct gggcgtgagc ctgcacgacc tgccccctcc
     5881 ggtccgggtg gtggtgaacg gcgagccggt ctcgctgagc gacggcacca cgttcggggg
     5941 agccatccgc atgatgcatc tgcgcccgcg cccagggcgc ctgctggacg tgcagggagg
     6001 cgtcctggag gctcgggccg accccggccg catcctgctg aacggagacg tagcgcctcg
     6061 atcccggggc cttcggagcg gcgaccgcat cagcgtcgtc aacggcacgg accgcaccga
     6121 gggcacccgg cgcgtcgtgg accgcctgcc cggacgccat cccggtgacc cccagtacag
     6181 cctggccacc tcccggatgg agcagatcac ggtggtcggg cgtcggtccg gcaaggtcgt
     6241 ctcggtccgc taccgctcca tcggtcggag ccacgcccct cccgccgtgg ccctgacctt
     6301 cgacgacggc ccctggccga agggcacccg gggggtcctc accgttctgg agcggatgca
     6361 tgccaaagcc acgttcttca tgatcggcta cctggtcgat cgttacccgg ccatcgcccg
     6421 ggacgtcgcg cgggccggca tggcggtggg caaccactcc tgggaccacg tggaggaccc
     6481 tccgttcgcg aagctgaagc cgcatcgcct ccacacagag ctggcccagg cgaaccaggc
     6541 gatcggccga gccaccggcg tccatcccac gctcttccgg ccgccggccg gcagctggga
     6601 cccggaggtc ctgaccgtcg cgaaccggct gggcatgcgc atcgcgctgt gggacgtgga
     6661 cccacgcgat tgggccgcct cggccacgcc caggtccatc gccgccaacg tcctccgcaa
     6721 cgtccgtccc ggagccgtgg tcgacctcca cgacggcggg ggggacgcga aggccaccat
     6781 ccgggccctg ccgaagatca tccgagggat ccgcaagatg ggcctgcagc tcgtcaccat
     6841 ggatcagcag ctcccacccg gcccgaggta ggagccgcgc tccgcgcggc tgggtagggt
     6901 ggcggtgatg gagcgcgcca ccgtcctgtt cgacctcgag tgcggcttct gccggtggac
     6961 cgcagaccgg ctcgccgcct gggatcgtcg tggccgcctc cggttcgtcg ccctgcagga
     7021 gcccgaggcc gaccgcctgc tcccgggcat gcccgaggag cgcaagatgg gctcatggca
     7081 cctggtcctg ccgaacggcc gtgtgcgctc ggccggcgac gccgtggcac ccctgatgcg
     7141 ccggctcccc gggggtgggc ccatcgccgc cctggccacc gcgctccccg gtcccaccga
     7201 gcgcctctac gcgttcgtct cccggcaccg cgacggactg gcccaggtgc tgggtccaag
     7261 gcgatcctgc gtggacccgc gctcggctcg acgaccgggg gaccgcccga acggcaagcg
     7321 ggctagagct cgctgagagc gggggccagc tcctcgagct gccccaggca cacgtcgtcc
     7381 tccgcttcag cgaccacctg caggcagacg tggtcggcgc cggcctcgag gtgctctcgc
     7441 acgcgggcca cgatccgatc cacgtcgccc cacgccacca cggcatcgat caaccggtcg
     7501 cttcctcccc cggcgatgtc gtggtctccc caacccatcc tcttgaggtt ccgcgcgtag
     7561 ttcggcgagg cgaggtaccc gtcggcgaac gtccgagcga tcccccgggc ccgggacgga
     7621 tcggtctgga ggacggcggt ctgctccacg gccagcacgg gatcggggcc gagccgggcc
     7681 cgggcgaacg ccgtgtgctc gaccggcacg aagtaggggt gggcaccctc ggcgcgctcg
     7741 gcggcgagct ccagcatgcg agggccgagc gccgccagca cccgccagag cgggccggca
     7801 ggctgtggcg ccgaggtggg agcggcgtcc atggcgtcga ggtactcgcg catccgcgag
     7861 taggggcgct cgtaccgatg cccgcgccga tcgacggcgt accggtggct gaccccgatc
     7921 cccaagagga agcgcccggg atgagcatcg gagagggcgc gccagccgtt ggccatggcc
     7981 acgggatcac gggcccagat gttcgcgatc ccggtaccca ccaccgcgcg ctcggtggcc
     8041 cccagcagca gagccgactg ggcgaacgcc tccttgctgt ccacggcctc ggggaaccag
     8101 agcacccggt agcccatggc ctcgatccgt tcggccgccg tccgttcctc ggcggccgaa
     8161 cgggtctcga gggtgaagct ccagacgccg gtccggccca gggccccgcg cagcgctcgc
     8221 cgatccgccg gcacgcctgc tcccctcagc tgtcgtcgcg cggcacgggc tccgcggcgg
     8281 gcgccacgcc tacgggctcg agggccggct gaagcacccg ctgggtcggc tcgcggccct
     8341 ccacgtcgat ctgcgggagc caccggagcc accgcggcag gtaccagttc aggtcgccga
     8401 gcagccgcat gccggccggg accaggagcg tccgcacgat ggtggcgtcc atgaacacgg
     8461 ccacagccag gccgaagccc atctgctgca gcgccccgat acgaccggcc gcgaagcccg
     8521 tgaacacggc caccatgatc agggcggcgc ccgtgatgat gccggccgtg gtccgaagac
     8581 cgtacgcgac ggcctccgtg ttgtggccga tccggtcgta ctcctcgcgg atcctggtca
     8641 gcaggaacac gtggtagtcc atggacagcc cgaa
//
LOCUS       DASQQQ010000094         2448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_306663, whole genome shotgun sequence.
ACCESSION   DASQQQ010000094 DASQQQ010000000
VERSION     DASQQQ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306663"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..1529)
                     /locus_tag="VE646_03920"
     CDS             complement(219..1529)
                     /locus_tag="VE646_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79174.1"
                     /translation="MTFGLERIDGEPLPLVTYENTITTVEATAGIIALEADGTFLAST
                     TFLTTPRHGAATETTFTSGTYAWTDATLHLSYVDGTTDQGVLDGALLTLASGGSTFEF
                     RRLEAPFELAAAYTFPDLAGDDFIQRFAFTGDGTLWVTSFGGSIVRVRSGKTTVWDAA
                     TDLGGERASGPFVDHADRVWIALGNGYAVHDGQSWTRFDAPAALTPGTSIGHVAVSGA
                     GDVLLAAGHEGVGGLLLRTAGGWMALTPDNSLLPNHVTRSVAVDPAGDFWVASARTSR
                     PGGLSRISGGVLVEVFDAEAGLLTDGAETIAVGGGGVVVGFDDFLHPEPGADGGIQVI
                     PTGGGPVTSWFPAVTGLTSSRVHSLAVTDAGDIWFVTTLDEAQPTCATCFSGIGRLDA
                     AGAFEVVSALNSDVPPNGYFAGIASDPSGVVYVGTEREIYRVVR"
     gene            complement(1878..>2448)
                     /locus_tag="VE646_03925"
     CDS             complement(1878..>2448)
                     /locus_tag="VE646_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HZD79175.1"
                     /translation="VVILAGVVVTAGAGLTSILPAALGGVVLMVFTGCVKIEELYREM
                     DWSVVFLLAGLIPLGLAMDETEAADWMGQVVGSALEPLPPVVAVGLFYLMTSLLTEVM
                     SNNAAAVVLTPVALVTAGALDMNPYALLVAVMFGASASFMTPIGYQTNMLVYGPGGYR
                     FLDFLKVGGPLNLILLVSAALLIPLFWGG"
BASE COUNT          395 a          863 c          861 g          329 t
ORIGIN      
        1 gtcgtggcgg atcgaggcgg gccggcaccg catcgtggcg gtgtggccgt cgggcgcgcg
       61 cgactcggtg gaggtgcggg gcgagggcac ccggtagtgc cgtcgcggtc atgtccagcc
      121 cgcagccgtc acctggcgtg ccctccatcg cttgaagaag gcggaagggc tgatccggga
      181 gctatgacga cgcgcccgcc ggctcgtgcg ccacccgctc agcggacaac gcgatagatc
      241 tcccgctccg tccccacgta caccaccccc gacgggtcgc tcgcgatccc cgcgaagtag
      301 ccgttcgggg ggacatcgga gttcagggcg gagaccacct cgaacgcgcc agcggcgtcg
      361 agccggccga ttccgctgaa gcaggtggcg caggtgggct gcgcctcatc gagggtcgtg
      421 acgaaccaga tgtcccccgc gtcggtgacg gccagggagt gaacgcggct cgaagtgagg
      481 ccggtcaccg cggggaacca cgaggtgacc gggccgccgc cggtgggaat gacctggatg
      541 cccccgtccg cgcccggctc ggggtgcagg aagtcgtcga agcccaccac gacgccgcca
      601 ccgcccacgg cgatggtctc ggccccgtcc gtcaggagcc ctgcctcggc gtcgaagacc
      661 tccaccaaca cgcctccgga gatgcggctc aggccacctg gccgcgaggt tcgggcggag
      721 gccacccaga agtcgccggc cgggtccacc gccacgctcc tggtcacgtg gttcgggagc
      781 agcgagttgt ccggcgtgag ggccatccag ccacccgccg tccgcagcag gagcccgccc
      841 accccctcgt ggcccgctgc caggagtacg tcaccggccc cactgaccgc cacgtggccg
      901 atgctcgtcc ccggcgtgag tgcggccggc gcgtcgaagc gcgtccagct ctggccgtcg
      961 tggacggcgt agccgttgcc cagcgcgatc cagaccctgt ccgcgtggtc gacgaagggg
     1021 ccgctcgccc gctcgccccc gaggtcggtt gccgcgtccc agaccgtggt cttccccgag
     1081 cggacccgaa cgatcgagcc gccgaacgac gtgacccaga gcgttccatc ccctgtgaac
     1141 gcgaagcgct ggatgaagtc gtcgccggcc aggtcgggga aggtgtacgc ggccgccaac
     1201 tcgaacggcg cctccagccg ccggaactcg aacgtgcttc cgccgctggc gagcgtgagg
     1261 agcgcgccgt cgagcacgcc ctggtccgtg gtgccgtcca catacgaaag gtgcagcgtg
     1321 gcgtcggtcc acgcatacgt accggaggtg aacgtcgtct cggtggccgc gccgtggcgc
     1381 ggtgtggtga ggaaggtcgt gctggccagg aaggtgccgt ccgcttcgag ggcgatgatg
     1441 cccgccgtcg cctcgaccgt ggtgatcgtg ttctcatagg tgaccagggg cagcggttcg
     1501 ccgtcgatgc gctcaaggcc gaaggtcacg ggccccgcgg ttcccgggcc gctgacgtct
     1561 ccgccacacg cagccgctcc cagagccccg agcaacgcca cgcaaatcgc acttcgcatg
     1621 cttcgactcc ggcgctttcg ggtagagtcg actcgaggac cacgcggccg ggaccaggtg
     1681 gtccgcttca agaataaggt gatggccgag ggcggcgaag tgccggagta cccaccgcag
     1741 gtgggtccgg taggaacgag gccgatgctg ccgccattca ccccgttgat cgcggccggt
     1801 ggcgcggagc gcagcggatt cgaggacagg gcggcgacat ccaggaggtc ccgttgtacc
     1861 cgcgagtcgc tcgcccctca ccccccccag aacagcggga tcagcagggc ggcgctcacc
     1921 aggaggatga ggttgagggg tccccccacc ttcaggaagt cgaggaagcg gtagccgccg
     1981 ggtccgtaga ccagcatgtt ggtctggtat ccgatgggcg tcatgaagga ggcggaggcc
     2041 ccgaacatca ccgccaccag cagggcatag gggttcatgt ccagggcccc ggccgtcacc
     2101 agcgcgacgg gggtcaacac caccgccgcg gcgttgttgg acatgacctc ggtgagcagg
     2161 ctggtcatga ggtagaacag gcccaccgcc accaccggcg ggagaggctc gagcgcgctc
     2221 ccgaccacct gccccatcca atcggcggct tcggtctcgt ccatggccag gcccagggga
     2281 atcagccccg ccaggaggaa gaccacgctc cagtccatct cccggtagag ctcctcgatc
     2341 ttcacgcagc cggtgaagac catgaggacg acgccgccga gggccgccgg gaggatggag
     2401 gtcaggccgg cccccgccgt cacgaccacc ccagccagga tgaccacg
//
LOCUS       DASQQQ010000095         2061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_172114, whole genome shotgun sequence.
ACCESSION   DASQQQ010000095 DASQQQ010000000
VERSION     DASQQQ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172114"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="VE646_03930"
     CDS             complement(<1..1289)
                     /locus_tag="VE646_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZD79176.1"
                     /translation="MKASGRSKISVPTKAGGPGWATGMAGRQEIDLRGLERDLSEAVA
                     GEVRFDRATRALYATDSSNFRQPPIGVVIPRTTRDVVATHEVCHAYQAPILARGCGTS
                     LSGETVNYAVVMDFSKYLHGIMDIDSERRLVRVQPGAINEKVNEATGRYGLVFGPDPS
                     THAYCTIGGNIGNNSCGIHSVQARFSGEGSRTSDNVHELEILTYDGTRMRVGKEEEGD
                     IDRIVAAGGRRGEIYAKLRDLRDRYADRIRERYQDIPRRVSGYNLDELLPEKGFNVAR
                     ALVGTEGTCVTVLEATLQLVPNPRHRALLVIGYEDIFSVGDHVAKIMAHRPMGCEAID
                     HMLFKDLEQLGIQTSDLPLLPEGEAWVLVEVGGETQEEADGLARDLMRDLDKDPDPPK
                     GSSLFDDLEQREKIWRIREAGLAGTAFPPDGRDHWPG"
     gene            complement(1345..>2061)
                     /locus_tag="VE646_03935"
     CDS             complement(1345..>2061)
                     /locus_tag="VE646_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011031648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZD79177.1"
                     /translation="DHVPIYGSGGFTSYSLERLAEQLGGWVEMGIARVKMKTSRHPEE
                     DPRRLDAVRKAIGPEPELFVDANGALSRKQALSWAERFRHEWGVSWFEEPVSSDDLEG
                     LRLLRDRGPGGMDIAAGEYGFLLADFRHLLAAGAVDCLQADVTRCGGITGLLRVSGLC
                     DTHTIDLSGHCAPAVSAHALCGVIHLRHLEYFHDHVRIEGLLFDGTLDPQDGELRPDR
                     SRPGLGLELKREEAQRFRVR"
BASE COUNT          288 a          777 c          656 g          340 t
ORIGIN      
        1 cacccgggcc agtgatcgcg cccgtccggc gggaaggcgg ttccggccag cccggcctcg
       61 cggatccgcc agatcttctc ccgctgctcc aggtcgtcga acagcgaact gcccttgggt
      121 gggtcggggt ccttgtccag atccctcatc aggtcccgcg cgagtccatc ggcctcttcc
      181 tgcgtctccc cccccacctc caccaggacc caggcctccc cctcgggcag cagcggcagg
      241 tcgctggtct ggatccccag ctgctcgagg tccttgaaca gcatgtggtc gatcgcctcg
      301 cagcccatcg ggcggtgggc catgatcttc gccacgtgat cgcccacgct gaagatgtcc
      361 tcgtacccga tcaccagcag cgcgcggtgc cgcgggttcg ggacgagctg gagcgtcgcc
      421 tccaggacgg tcacgcaggt gccctcggtt cccaccagtg cccgagccac gttgaagccc
      481 ttctccggca gcagctcgtc caggttgtag cccgaaaccc ggcgagggat gtcctggtac
      541 cgctctcgga tccggtccgc gtagcggtca cgcaggtcgc gcagcttggc gtagatctca
      601 ccgcggcggc cgcccgccgc aacgatccgg tcgatgtctc cttcctcctc cttgcccacg
      661 cgcatccgcg tgccgtcgta ggtcaggatc tcgagctcgt ggacgttgtc cgaggtccgg
      721 ctgccctccc cagagaagcg ggcctgcacc gagtggatcc cgcaggagtt gttgccgatg
      781 ttgccgccga tcgtgcagta ggcgtgggtg gaggggtccg gcccgaacac cagcccgtaa
      841 cgcccggtgg cctcgttcac cttctcgttg atcgcgccgg gctggacccg caccagccgg
      901 cgctcgctgt cgatgtccat gatcccgtgc aggtacttcg agaagtccat gaccacggcg
      961 tagttgaccg tctcgccgga caggctcgtg ccgcaccccc gggccaggat aggggcctgg
     1021 taggcgtggc agacctcatg ggtggccacg acgtcacggg tcgtccgagg gatcaccacg
     1081 ccgatcggag gctggcggaa gttcgaggaa tccgtcgcgt acagggctcg ggtggccctg
     1141 tcgaaccgca cctctccggc cacggcctcc gacaggtccc gctccagacc ccggaggtcg
     1201 atctcctgtc gcccggccat gcccgtcgcc caccccggac caccggcctt ggtcggtacg
     1261 ctgatcttcg atcgacccga cgccttcatg agctcgtcct cccccgtcgc cggcgttcgt
     1321 ccggcctcgt cgtcggcggc ttcgtcaccg cacgcggaac cgctgggcct cctcccgctt
     1381 cagctccaga cccagtccgg gccgagaacg atccgggcgc agctctccgt cttgcggatc
     1441 aagggtcccg tcgaagagca gaccctcgat ccgcacgtga tcgtggaagt actccaggtg
     1501 gcgcaggtgg atcacgccgc acagggcgtg cgccgagacg gccggggcgc agtgccccga
     1561 gaggtcgatc gtgtgcgtgt cgcacaggcc gctcaccctc agcaggccgg tgatgccgcc
     1621 acaccgcgtg acgtcggcct gcaggcagtc gaccgcgccc gccgccagca gatgccggaa
     1681 gtcggccagc aggaacccgt actcgccggc ggcgatgtcc atccctcccg gccctcggtc
     1741 ccggagcagc cggagcccct ccaggtcgtc ggagctcacc ggttcctcga accacgagac
     1801 cccccactcg tgccggaacc gctccgccca ggacaaggcc tgcttgcggc tgagcgcccc
     1861 gttggcgtcc acgaacagct cgggctcggg tccgatcgcc ttgcggacgg cgtcgagccg
     1921 ccgcgggtcc tcctccggat gccgcgaggt cttcatcttg acccgggcga tgcccatctc
     1981 gacccaaccg cccagctgct ccgccagccg ctcgagcgag tagctggtga accctccgct
     2041 gccgtagatc ggcacgtggt c
//
LOCUS       DASQQQ010000096         4066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_200480, whole genome shotgun sequence.
ACCESSION   DASQQQ010000096 DASQQQ010000000
VERSION     DASQQQ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200480"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VE646_03940"
     CDS             <1..269
                     /locus_tag="VE646_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="menaquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HZD79178.1"
                     /translation="FLGLQELLRYFHPMVAGISIPTFILVGLAAVPYIDRNPSTRPGD
                     RKLAITMFTMLFMFGATLTIAGSFFRGTGYNWVWPWAQGVFFEL"
     gene            266..1000
                     /locus_tag="VE646_03945"
     CDS             266..1000
                     /locus_tag="VE646_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HZD79179.1"
                     /translation="MTTILALSSGTIAALVVMGVIDLFAVVFGLSFARARRAEREAAM
                     AAPGQPAAPGPKAPKPVSRREFFRRSLVTSLAVFGAQFGGATIAFLWPNLKGGFGSVI
                     AAGKIGDIKSFIDANNQPFYQGAGRFYVVEYKGKASGSVDYEGEGVATQGIMPLYQRC
                     VHLGCRVPFCQQSQWFECPCHGSKYNYAGEYRLGPAPRGLDRFKLTVDAGGNVFVDTS
                     QIILGPPRGTDTIDEPPQGPFCVAPG"
     gene            1007..1741
                     /locus_tag="VE646_03950"
     CDS             1007..1741
                     /locus_tag="VE646_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZD79180.1"
                     /translation="MLALSKAQGIILIIGGAVFVLIAGGALLSRGRGRRAEAPPEIPR
                     GMRPGPSDPDLETPLLQKLQGWGVLLVVFFVIWIPLTWLSEPSRNYDQEKALLTDSVA
                     RGKAGVQLFSEENQGGVGCVRCHGSELTGSKIVDTTTGAVINTPNLTTVCGGPWTGHP
                     LIYSLNDIYITIEQGRNIMPSWSIRYAGAMDDQQINDIVNYIVSIQDKGQVTFKHNVC
                     VNPDATKAAVDEFGPPPSSPTTNAAP"
     gene            1738..2487
                     /locus_tag="VE646_03955"
     CDS             1738..2487
                     /locus_tag="VE646_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79181.1"
                     /translation="MSPLAAGTCIGFHIDCGILVKGGLGVGMAFVLFIGSVLLLLSAV
                     LGRKMGYLVLAVAFFGWMAIFSGLWVSGYFISQGPTTVKDLGPRGAEPAWVVEAAGTN
                     VSSYPYQEYKDYPGGGWTTPSPGLSASVQSVTGAIQAYLADQANEEAGKSEFEPGAFQ
                     TTDFTVQDIKFDTSGKVSLAAAHAYYNSGGPLITLYLRHDSGSVPRYSWMFLAGSLLG
                     LVIHLPFLDRVERKRKEILTGGTAPPWYGPA"
     gene            complement(2658..3341)
                     /locus_tag="VE646_03960"
     CDS             complement(2658..3341)
                     /locus_tag="VE646_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015658712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HZD79182.1"
                     /translation="MSERPIPEATVGRLPIYLRALVELAELGHATVSSEMLAEAAGVN
                     SAKVRKDLSHLGSYGTRGVGYDVAYLIHQVRRELGLTQHWPIVIVGIGNLGHALANYR
                     GFAARGFRIAALVDADPLKVGEPVAGLHVRPVEDLPDIVRQNDGLLGVICTPAAEAQK
                     VADRLVEAGIRSILNFAPAVLQVAEGVSVRKVDLAIELQILAYYEQRKVALDQVRSRG
                     RAMADLTEL"
     gene            complement(3402..3641)
                     /locus_tag="VE646_03965"
     CDS             complement(3402..3641)
                     /locus_tag="VE646_03965"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HZD79183.1"
                     /translation="MDLEVVMYVRPGCGLCEEAREVILAEHRQTPFTYREVDISGRDA
                     LELEYGVRIPVVEIDGRELFELRVPAAEFAAAVRR"
     gene            3708..>4066
                     /locus_tag="VE646_03970"
     CDS             3708..>4066
                     /locus_tag="VE646_03970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZD79184.1"
                     /translation="MQEDDRLDLELHRRLEIGDREAFEQLYRRYASSAYGLALRVTGQ
                     DPLAQEVVSDAFLALWRAPQAFDATRGSFRAFFLSLVHHRAVDTVRREERLRKRAERS
                     SNLEPRVDEDVAEGVVE"
BASE COUNT          618 a         1454 c         1360 g          634 t
ORIGIN      
        1 acttcctggg cctgcaggag ctgctgcgct acttccaccc gatggtggcg ggcatctcga
       61 ttcccacgtt catcctggtg gggctcgcgg ccgtgcccta catcgaccgc aaccccagca
      121 ccaggcccgg tgatcgcaag ctcgccatca ccatgttcac gatgctgttc atgttcggag
      181 ccacgctcac catcgccggc tccttcttcc gcggcaccgg ctacaactgg gtctggccgt
      241 gggcacaggg ggtgttcttc gagctatgac gaccatcctg gccctgtcat ccgggaccat
      301 cgccgccctc gtggtgatgg gcgtgatcga cctgttcgcg gtcgtcttcg gcctctcgtt
      361 cgcccgcgca cgccgggccg agcgcgaggc cgccatggcc gcaccgggcc agccggccgc
      421 ccccgggccc aaggctccca agcccgtctc gcgccgcgag ttcttccggc ggtccctggt
      481 cacctccctg gcggtgttcg gcgcgcagtt cgggggcgcg accatcgcct ttctctggcc
      541 gaacctcaaa gggggcttcg ggtccgtgat cgcggccggc aagatcgggg acatcaagag
      601 cttcatcgac gcgaacaacc agcccttcta ccagggggcc ggccgcttct acgtggtcga
      661 gtacaagggc aaggcctccg gcagcgtgga ctacgagggc gagggggtgg ctacccaggg
      721 gatcatgccc ctgtaccagc ggtgcgtgca cctggggtgc cgggtgccgt tctgccagca
      781 gtcgcagtgg ttcgagtgcc cctgccacgg ctcgaagtac aactacgcgg gcgagtaccg
      841 cctgggcccg gccccccgag gcctggaccg cttcaagctc acggtcgatg ccggcggcaa
      901 cgtgttcgtg gacacgtccc agatcatcct gggaccgccg cggggcaccg ataccatcga
      961 cgagccgccg cagggtccgt tctgcgtggc gccgggatag gggacgatgc tcgcactctc
     1021 caaggctcag ggcatcatcc tcatcatcgg gggggcggtc ttcgtcctca tcgcgggcgg
     1081 ggccctgctc tcgcgcggcc ggggccggcg ggccgaggcg ccaccggaga tcccccgcgg
     1141 catgcgaccc ggcccctccg accccgatct ggagacgccg ctgctgcaga agctccaagg
     1201 gtggggcgtc ctgctggtgg tgttcttcgt gatctggatc ccgctcacct ggctgtcgga
     1261 gcccagccgg aactacgatc aggagaaggc gctgctcacc gactcggtgg cccggggcaa
     1321 ggccggggtg cagctgttct ccgaggagaa ccagggcggc gtggggtgcg tgcgctgcca
     1381 cgggtccgag ctcaccggct ccaagatcgt ggacaccacc acgggggcgg tgatcaacac
     1441 gccgaacctg accaccgtgt gcgggggacc ctggaccggc cacccgctga tctacagcct
     1501 gaacgacatc tacatcacga tcgagcaggg ccggaacatc atgccgtcct ggagcatccg
     1561 gtacgccggc gccatggacg accagcagat caacgacatc gtgaactaca tcgtgtcgat
     1621 ccaggacaag ggccaggtca ccttcaagca caacgtctgc gtcaatccgg acgccacgaa
     1681 ggccgccgtc gacgagttcg ggccaccgcc gtcgtccccg accaccaacg ccgcaccatg
     1741 agcccgctcg ccgccggcac ctgcatcggg ttccacatcg actgcggcat cctcgtgaag
     1801 ggcgggctgg gcgtggggat ggccttcgtc ctgttcatcg gcagcgtgct gctgctgctc
     1861 tccgccgtcc tggggcgcaa gatgggctac ctggtgctcg cggtggcctt cttcggctgg
     1921 atggccatct tctccgggct gtgggtgagc gggtacttca tctcccaggg gcccaccacc
     1981 gtgaaggacc tcgggccccg tggcgccgag cccgcctggg tggtggaggc cgccgggacc
     2041 aacgtgagca gctaccccta ccaggagtac aaggactatc cgggcggcgg gtggaccacc
     2101 ccctcgcccg gcctgtcggc ctcggtccag tcggtgaccg gtgcgatcca ggcctacctc
     2161 gccgaccagg ccaacgagga ggcggggaag tcggagttcg agcccggcgc cttccagacc
     2221 accgacttca ccgtgcagga catcaagttc gacacctccg ggaaggtgtc cctggccgcc
     2281 gcgcacgcct actacaacag cggcggcccg ctgatcacgc tgtacctgcg ccacgacagc
     2341 ggcagcgtgc cgcggtactc gtggatgttc ctggccggct cgttgctcgg cctggtgatc
     2401 cacctcccgt tcctggaccg cgtcgagcgc aagcgcaagg agatcctgac cggcgggacc
     2461 gccccgccct ggtacggacc cgcctaggag aggagacgga gtgctggcag ccgaggctcg
     2521 cttgctggat cagggcgtcg tgcagttcat cctgttcggc gtcatggtcc tggccgtgtt
     2581 catcacgctc tggttcacca tcgcgaagta gccggacccg gccgttccac caccgccgca
     2641 cgccatcgtc ggcgtgctca gagctccgtc aggtccgcca tggcccggcc ccggctccgg
     2701 acctggtcca gcgccacctt gcgctgctcg tagtaggcca ggatctgcag ctcgatcgcc
     2761 aggtccacct tccggacgga gacgccctcg gccacctgta ggacggcggg cgcgaagttc
     2821 aggatcgagc ggatcccggc ctccaccagc cggtccgcga ccttctgcgc ctcggcggcc
     2881 ggcgtgcaga tgacgccgag caggccgtcg ttctgccgga cgatgtcggg caggtcctcc
     2941 accgggcgga cgtgcagccc ggccaccggc tcacccacct tgagcgggtc ggcgtccacc
     3001 agcgccgcga tgcggaaccc gcgggcggcg aacccccggt agttggccag ggcgtggccg
     3061 aggttcccga tacccacgat cacgatgggc cagtgctggg tcagccccag ctcccgacgg
     3121 acctggtgga tcaggtaggc gacgtcgtag ccgacccccc gcgtcccgta ggagcccagg
     3181 tgcgagaggt ccttccgcac cttggcggag ttcacgccgg ccgcttcggc cagcatctcg
     3241 gacgacacgg tggcgtgacc cagctcggcc agctccacca gtgcgcggag gtagatcggc
     3301 agacgcccca ccgtggcctc ggggatgggg cgctcgctca ccgcccgatg gtaccgggtc
     3361 gagggtccgc gcacaaacgc gcaagctctg caccggaacg atcagcgccg cacggccgcg
     3421 gcgaactcgg ccgccggcac gcggagctcg aacagctcgc gtccgtcgat ctcgaccacc
     3481 gggatccgga ccccgtactc gagctccagg gcgtcccgac cggagatgtc cacctcccga
     3541 taggtgaagg gcgtctgccg gtgctccgcc aggatcacct cgcgggcctc ctcgcagagc
     3601 ccgcaccccg gacggacgta catcaccacc tcgagatcca cggatgagca tgctacccgg
     3661 ccggggctgg ggccaccggt cgtgcggccg gtgacacaat cggagagatg caggaggacg
     3721 accgcctgga cctcgagctt catcggcgcc tggagatagg cgaccgggag gccttcgaac
     3781 agctgtaccg acggtacgcc tcctcggcct acggcctggc gctccgggtg acgggtcaag
     3841 atcccctggc ccaggaggtc gtgagcgacg ccttcctggc cctgtggcga gcaccccagg
     3901 cgttcgacgc cacacggggg tcgttccggg ccttcttcct ctccctggtg caccaccggg
     3961 ccgtcgacac ggtgcgtcgg gaggagcggc tccggaaacg cgctgagcgt tcatcgaacc
     4021 tcgagccccg cgtcgacgaa gatgtagcgg agggcgtcgt ggagga
//
LOCUS       DASQQQ010000097        10339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_88615, whole genome shotgun sequence.
ACCESSION   DASQQQ010000097 DASQQQ010000000
VERSION     DASQQQ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10339
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88615"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VE646_03975"
     CDS             complement(<1..233)
                     /locus_tag="VE646_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79185.1"
                     /translation="MKLSSKMRWVGRGAASGAKAGAKLLGTGAKGGAKVASKGAKAKA
                     ESMGRQAKHKALERRIDRLDEENQELRIENRVL"
     gene            complement(333..905)
                     /locus_tag="VE646_03980"
     CDS             complement(333..905)
                     /locus_tag="VE646_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012998528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZD79186.1"
                     /translation="MAEFHVLHQGYLRDDEDRVGGTTSFVRDGDVLVVIDPGMCRDRA
                     ALLTALTGLGVDPEGVTDVVISHHHPDHTLNVALFPQARVHDHWAWYRDDLWISRPAE
                     GVELAPGIRLLETPGHTPQDISTAIQTDAGLIVCTHLWWTADGPDEDPYAPDPELVHR
                     NRGRVLELPGLLRIVPGHGPPFEPGPNTPR"
     gene            complement(932..1744)
                     /locus_tag="VE646_03985"
     CDS             complement(932..1744)
                     /locus_tag="VE646_03985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZD79187.1"
                     /translation="MSVPIDLLRRIDAYLDAAPRTGATGEPVGPFTLFVNGGGGWRYY
                     ARPTPGARPVTPEDVVAVRERQRALGQPEAIEWVLDVSPGVGAAAGAAGMQVAERPLM
                     HLATPDFRAAQTPHGGMVRVLDPNEDPAIAMAVAELGFASEGTDPGPVGYRPARILAA
                     RLERGALEFVGDRLRRGLTVTALATSGGEPAAVGSHQPLHDMSEIVGVTTLPAFRRRG
                     LGAAVTSVLVRDALERGADTVFLTAGNEEIARVYERVGFRTIGRVGVATAPD"
     gene            1873..2193
                     /locus_tag="VE646_03990"
     CDS             1873..2193
                     /locus_tag="VE646_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017602755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZD79188.1"
                     /translation="MFQHVDPRWMDHPGWWGSFAGFIPLLLFLVVIGVLVWAVFRVTS
                     HPRTAPPGPWVRPAPPAPTQDRALEEVRVQYARGEITREEFLQRTEDLSGVRPGQGPP
                     GQAG"
     gene            2206..2922
                     /locus_tag="VE646_03995"
     CDS             2206..2922
                     /locus_tag="VE646_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD79189.1"
                     /translation="MPSTRSQRSSPDARVLVVDDESYITDLVATALRYEGFEVESAAD
                     GRTALAKVESFRPDLLVLDVMLPDVDGFQVQERLVDRGRRVPVLFLTARDATEDKVRG
                     LTIGGDDYVTKPFSLEELVARIRAILRRVGGAPEGTGRLRFEDLEMDEDTHEVWRAGR
                     AVELTPTEFNLLRLLMMNPRRVLSKAQILDHVWNYDFGGDASIVETYISYLRRKVDLV
                     DPHLIHTVRGVGYVLRLPSG"
     gene            2927..4375
                     /locus_tag="VE646_04000"
     CDS             2927..4375
                     /locus_tag="VE646_04000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZD79190.1"
                     /translation="MSLRARLLITTLLIAAAGLVVADVTTYRFLSSFLVHRVDEQLVA
                     ARESAAHALSVEARFGRSAPPPDVQVPDGTYAQVRDAAGRTVIPWRCFCYGMTTPSPP
                     SLPAVLPGSTGSSRSVRTFSTSPVAGGSPHYRVLATALVPDGGTLVVAIPLTDVVATL
                     HRLLFLEVVVSVAVLAVLGGLSLWLVRLGLRPLEGMGETAGAIAAGDLSRRVEPCDER
                     TEVGRLGMALNAMLAQIEAAFEERRASEQRLRRFVADASHELRTPLTSIRGYAELFRR
                     GADTHPEDLAKSMERIEAEASRMGVMVDDLLLLARLDQGRPLEREPVDLPKIVTDSVE
                     AARAAEPDRPIELRSVDEAVVLGDPHRLRQVVDNLLQNVRVHTPAGAPAHVDVSSEGG
                     WVELGVTDEGRGLDPEATGRAFERFFRGDPSRSRGNGGAGLGLSIVAAIVQAHGGQVR
                     AESPDGGGARFVVRLPAVPVEQPSELPPPPGE"
     gene            4430..5263
                     /locus_tag="VE646_04005"
     CDS             4430..5263
                     /locus_tag="VE646_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZD79191.1"
                     /translation="MAGAIGFICITCGVQHAPSEIPPERCAICEDERQYVGDGGQRWT
                     TLAEMRGRYRNTFMELEPDLVSIRTMPGFAIGQHALLVRTRAGNVLWDCLSYLDDETV
                     ARVRELGGIAAIAISHPHFYDSCVEWSSAFGDAPIYLPAVDREWVMRPSPSIVHLEGD
                     QVEPVPGVRVIRVGGHFHGSTVLHWAAGAEGRGALLTGDSLSVVADRRFVTVMYSYPN
                     RIPLSAGAVRDVGVRALRHPFDRLYGGWRGDVIEHEAMQAVRASVERYAGMVEGSWPR
                     R"
     gene            5377..6571
                     /locus_tag="VE646_04010"
                     /pseudo
     CDS             5377..6571
                     /locus_tag="VE646_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686635.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-independent formaldehyde
                     dehydrogenase"
     gene            complement(6596..7084)
                     /locus_tag="VE646_04015"
     CDS             complement(6596..7084)
                     /locus_tag="VE646_04015"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HZD79192.1"
                     /translation="MPVLVTYASKHGATEGIAEAIGERLRERGIKTEVEPIGDRDDLG
                     AVDAVVVGSAIYMGSWMKEASAFLERHRDELDTLPVWLFSSGPTGEGPTEVGLADKQL
                     AKLAGLVAAREHHVFFGAIDPERLGFLERRIVKTVKAPTGDFRDWDEVRSFADRIAGG
                     LS"
     gene            7307..7534
                     /locus_tag="VE646_04020"
     CDS             7307..7534
                     /locus_tag="VE646_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79193.1"
                     /translation="MPDSVGKRKRRDVTAKKAAAREERRLARASRKRDREAGLIESGP
                     PIGPAEQSEWLPGHGEAAEAQEEEAAEASES"
     gene            7593..8081
                     /locus_tag="VE646_04025"
     CDS             7593..8081
                     /locus_tag="VE646_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZD79194.1"
                     /translation="MAEAVQPETDRSVGVRELGHVVLYVRDLSRSVTFYRDVLGWRQV
                     VGAETVGMPVAMFSGGRTHHELLLIEVGEGATPIPAGRRVGLYHFGLKVGDTDEELRT
                     MLTRLVDAGLQLTGTADHGMTHSLYLADPDGNEIELYVDVPGADWNDPEVLMSRPRPL
                     RL"
     gene            complement(8088..9284)
                     /locus_tag="VE646_04030"
     CDS             complement(8088..9284)
                     /locus_tag="VE646_04030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD79195.1"
                     /translation="MDSTRPAVRERIAVAATFSAHAVVAGTVGPWIPSLKARSGLDAG
                     ALGLALGAYAGGLVAGTRLAGPVVRRTGRGAVRTGIPLLAAALALLPLAGRLAWLAGI
                     FALLGLVSGLLDVAMNLEAAAVEARARRRVMSGMHGAWSASMLVGAAIASAGVAARVP
                     IEVHLAVVAILLAAGSWPLLRWLPPAGNEHPGRRPAADRGPPSPATSSVRSSPRSTGT
                     VVLLCLIPFAAFLTEGVAAEWSAVYLRSSAGTTPGVAGVGIVAFSAGMAASRFPGDRL
                     AARIEPSTVVGTGTAVAAAALGTALIAGGAAAALTAFTLVGLGVGPAVPLAFSEAARM
                     RRPRSGSVLGLAVTAGYLGSIAGPMAVGFTADRFGLRTALVIPVAMCLAAALGAGAIR
                     PRPAAR"
     gene            complement(9294..9635)
                     /locus_tag="VE646_04035"
     CDS             complement(9294..9635)
                     /locus_tag="VE646_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79196.1"
                     /translation="MDDGCREAHGVRGFALLKPARGDLDGATETMDLALTHCSRQRDT
                     HLWLRACVLDGLCAVAVAVRHLMAERWISDLISLTARSEMHQLSARAYLYRPDLLGGA
                     PPPDGRTGGRA"
     gene            9737..9805
                     /locus_tag="VE646_04040"
     CDS             9737..9805
                     /locus_tag="VE646_04040"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HZD79197.1"
                     /translation="MVQVERAYDPDNLFYLNQNIQP"
     gene            9851..>10339
                     /locus_tag="VE646_04045"
     CDS             9851..>10339
                     /locus_tag="VE646_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HZD79198.1"
                     /translation="MEVDLRAYQARIGYQGPVRPTADCLAELHLAHVGSIPFENLDIL
                     LGRPILLDLESLQAKLVEARRGGYCFEHNTLFAAVLETIGFDVTRLAARVGHEARAPA
                     PRTHMVLGVEAEGFQWLADVGFGGASILRPLPFEPGPVVEQFGWRFRLVDVDGLRVVQ
                     ALR"
BASE COUNT         1429 a         3611 c         3846 g         1453 t
ORIGIN      
        1 ttcaggacgc ggttctcgat ccggagctcc tggttctcct cgtccaggcg gtcgatccgg
       61 cgctccagcg ccttgtgctt ggcctgccgg cccatggact cggccttggc cttggcgccc
      121 ttgctggcca ccttcgcgcc gcccttcgct ccggtcccca ggagcttggc cccggccttg
      181 gcgccactcg cggctccccg tccgacccac cgcatcttcg aggacagctt catagcatcg
      241 gcctccttcc gccctctccg cagatggata cccacggcct ccgaccgcga accagggcgc
      301 acggccacca gctcgctccg gcgcgggggc gctcagcggg gggtgttcgg ccccggctcg
      361 aaggggggtc cgtggcccgg gacgatgcgg agcagaccgg gcagctccag cacgcggccg
      421 cggttgcgat gcacgagctc gggatcgggg gcgtacgggt cctcgtccgg accgtcggcc
      481 gtccaccaca gatgggtgca cacgatcagg ccggcgtcgg tctggatggc cgtggagatg
      541 tcctgtggcg tgtgtcccgg cgtctccagc agccggatcc ccggagccag ctcgacgccc
      601 tcggccgggc ggctgatcca gaggtcgtcg cggtaccagg cccagtggtc gtggacccgg
      661 gcctggggga acagcgccac gttgagcgtg tggtcggggt gatggtggga gatgaccacg
      721 tcggtgacgc cctcgggatc tacgcccagg cccgtcagcg cggtcagcag ggccgctcga
      781 tcccggcaca tcccggggtc gatcaccacc aggacgtccc cgtcccggac gaaggaggtg
      841 gtgccgccca cccgatcctc gtcgtcccgc agatacccct ggtgcagcac gtggaactcg
      901 gccatgcccg gccccttcct gtctcgttca ctcagtccgg cgcggtcgcc accccgaccc
      961 gcccgatcgt cctgaagccc acccgttcgt acacccgcgc gatctcttcg ttccctgccg
     1021 tcaggaacac cgtgtccgcc ccgcgctcca aggcatcccg gacgaggacc gaggtcaccg
     1081 cggccccgag ccctcggcgg cggaaggccg gcagggtcgt cacgcccacg atctcgctca
     1141 tgtcgtggag gggctgatgg ctgccgacgg ccgccggctc ccccccggac gtagccagcg
     1201 cggtgacggt caacccacga cggagccggt ccccgacgaa ttccagggct ccgcgctcca
     1261 gccgggcggc gaggatccgt gccggccggt acccgacggg acctggatcg gtgccttcgc
     1321 tcgcgaaccc cagctcggcg accgccatcg cgatggccgg gtcctcgttc ggatcgagga
     1381 cgcgcaccat gccgccgtgg ggcgtctgcg cggcgcggaa gtccggggtc gccaggtgca
     1441 tcagggggcg ttccgcgacc tgcatcccgg cggcgcccgc ggcggcgccc acgccgggcg
     1501 agacgtcgag cacccactcg atcgcttcgg gttgacccaa ggcgcgctgt cgctcgcgca
     1561 cggcaacgac gtcctccggg gtgaccggcc gggcccccgg cgtggggcgt gcgtagtaac
     1621 gccagccgcc gcccccgttc acgaacagcg tgaacggtcc gactggttcg ccggtcgcgc
     1681 cggtgcgggg ggcggcgtcg aggtacgcgt cgatgcgccg cagcaggtcg atgggtacgc
     1741 tcaccggagg cagagcctac cgccaccgcg tgggaagatg gcctccgacc ggttccctgc
     1801 ggcctccccg aggatcctca gcgaactccc aggttcgtgt ggtgcagtgg tggtcgagga
     1861 ggtggaggcg acatgttcca gcacgtggat ccgagatgga tggaccaccc ggggtggtgg
     1921 gggtccttcg ccgggttcat cccgctcctg ctcttcctgg tggtgatcgg cgtcctggtc
     1981 tgggccgtct tccgcgtgac ctcgcacccg cggaccgcgc ctcccggtcc ctgggtgcgg
     2041 ccggcgcccc cggctcccac ccaggaccgc gcgctggagg aggtccgggt ccagtacgcg
     2101 cgcggggaga tcacgcggga agagttcctg cagcggaccg aggacctgag cggggtgcga
     2161 cccggtcagg gtcctccggg ccaggccggg taaggggtcg ggacggtgcc gtcgacgcgc
     2221 tcgcagaggt cctcgccgga tgcgcgcgtc ctggtcgtgg acgacgagtc ctacatcacc
     2281 gacctggtgg cgacggccct gcggtacgag gggttcgagg tcgaatcggc cgccgacggt
     2341 cggaccgccc tcgccaaggt ggagtcgttc cggcccgacc tcctggtcct ggatgtgatg
     2401 ctccccgacg tcgacggctt ccaggtgcag gagcgattgg tcgatcgggg ccggcgcgtg
     2461 cccgtgctgt tcctcaccgc tcgggacgcc accgaggaca aggtcagagg gctgacgatc
     2521 ggcggagacg actacgtgac gaagccgttc agcctggagg aattggtggc caggatccgg
     2581 gccatccttc gccgggtggg tggcgcgccc gagggcacgg gccggctgcg gttcgaggac
     2641 ctggagatgg acgaggacac gcacgaggtc tggcgagcgg gccgcgccgt cgagctcacg
     2701 cccaccgagt tcaacctctt gcgcctgctc atgatgaacc cgcgacgggt cctgtcgaag
     2761 gcgcagatcc tcgatcacgt ctggaactac gatttcgggg gcgacgcctc catcgtcgag
     2821 acctacatca gctatctgcg ccggaaggtc gatctggtcg accctcacct gatccacacg
     2881 gtccggggag tgggctacgt gctgcgcctg ccctcggggt gaccccgtgt ccctgcgcgc
     2941 ccgcctgctc atcaccaccc tgctgatcgc ggccgcgggg ttggtggtgg ccgatgtgac
     3001 cacgtaccgg ttcctgtcct ccttcctggt ccaccgggtg gacgagcaac tggtggcggc
     3061 ccgggagtcg gccgcccacg ccctctcggt ggaggcgcgg ttcggccgct cggcccctcc
     3121 ccccgatgtc caggtgccgg acggcacgta cgcgcaggtc cgggacgccg cggggaggac
     3181 cgtgatcccg tggcggtgct tctgctacgg gatgaccacc ccctcgcctc cgtccctgcc
     3241 cgccgtgctc cccggctcga ccgggtcgtc gcggagcgta cgcacgttct ccacgtcgcc
     3301 ggtcgccggc gggtcacccc attaccgggt cctggccacc gcgctggttc ccgatggggg
     3361 aacgctggtg gtggcgatcc ccctcacgga cgtcgtggcc acgctgcatc ggctcctgtt
     3421 cttggaggtc gtggtcagcg tggccgtgct ggcagtcttg gggggcctgt ccctgtggct
     3481 ggtccggctg ggcctgcgtc cgctcgaggg catgggcgag acggccgggg cgatcgctgc
     3541 cggagacctg tcccggcggg tggagccctg cgacgagcga accgaggtgg ggcggctcgg
     3601 catggccctg aacgcgatgc tggcccagat cgaggcggcc ttcgaggagc gccgggcctc
     3661 cgagcagcgg ctgcggcggt tcgtggcgga cgcctcgcac gagctccgga cccccctgac
     3721 gtcgatccgg ggctacgcgg agctgttcag gcggggggcg gacacgcatc ccgaggatct
     3781 ggccaagtcg atggagcgga tcgaggccga ggcctcgcgc atgggcgtga tggtggacga
     3841 cctgctgctg ctggcccgtc tggatcaggg ccgcccgctg gagcgagagc ccgtcgacct
     3901 gcccaagatc gtcacggatt cggtggaggc cgctcgggcg gccgaacccg atcgaccgat
     3961 cgagctccgc tcggtcgatg aggccgtggt gctgggcgat ccccaccggc tgcgccaggt
     4021 ggtggacaac ctgctgcaga acgtccgggt gcacacgccg gccggcgccc cggcccacgt
     4081 ggacgtgtcc tcggagggcg gatgggtgga gctcggggtc accgatgagg gtcgggggct
     4141 ggacccggaa gcgacgggcc gggccttcga gcgcttcttc cgcggcgacc cctcgcggtc
     4201 ccggggcaac ggcggggccg ggctggggct gtccatcgtg gcggcgatcg tgcaggccca
     4261 cggcggccag gtccgcgccg agagccccga cggcggtggg gcccggttcg tggtccggct
     4321 cccggccgtg cccgtggagc agccatccga gctgccacct ccaccgggcg agtgaacgac
     4381 caggggcgcg ggcgcgagga cggctgcggt gccggggtag ggtgggctcg tggcaggggc
     4441 gatcggcttc atctgcatca cctgcggggt ccagcacgcc ccgagcgaga tcccgccgga
     4501 gcgctgcgcg atctgcgagg acgagcggca gtacgtgggt gacgggggcc agcggtggac
     4561 cacactggcc gagatgcgcg ggcggtaccg caacacgttc atggagctgg agccggatct
     4621 ggtgagcatc cggacgatgc ccgggttcgc catcggccag cacgccctcc tggtgcgcac
     4681 ccgggccggc aacgtgctgt gggactgcct gagctacctg gacgacgaga cggtggcgcg
     4741 ggtacgcgag ctcggaggga tcgcggccat cgccatctcg catccgcact tctacgacag
     4801 ctgcgtggag tggagctcgg cgttcgggga cgccccgatc tacctccccg cggtggaccg
     4861 agagtgggtg atgcgcccga gcccgtcgat cgtgcatctc gagggggacc aggtcgagcc
     4921 ggtcccaggc gtgcgggtga tccgtgtggg cggccacttc cacggctcca ccgtcctgca
     4981 ctgggcggcg ggggccgagg ggcgaggcgc gctcctcacc ggcgacagtc tgagcgtcgt
     5041 agcggatcgg cgcttcgtca cggtcatgta cagctaccct aaccggatcc ccctgtcggc
     5101 cggcgcggtg cgcgacgtgg gggtgcgtgc gctccgccac cccttcgacc ggctgtacgg
     5161 ggggtggcgc ggggacgtga tcgagcacga ggcgatgcag gcggtccgag cctcggtgga
     5221 gcgatacgcg gggatggtgg aggggtcctg gcctcggcga tgaccgcgcc gcccgcccgg
     5281 gacggccccc ggcggccttc gtttcctgtc tcgaaccggc gggtaggcgg ctgatctgcg
     5341 gatcgggcag ggggcgcgag cgcgaggagg tcgagcatga gggccgccgt ctacaagggt
     5401 ccgcgttccg tctccgtaga gcaggtggac gatccgaggc tcgaggagcc gaccgatgcc
     5461 ctgcttcggg tgacctccac ggcgatctgc gggagcgatc tgcacatgtt cgagggacgg
     5521 agctccgtgg aggaggggac ggtcttcggg cacgaggatc atgggggtga tcgaggaggt
     5581 cggaccgggc gtccacacgc tccgggccgg cgatcgggtg gtcctgccct tcaacatcgc
     5641 gtgtggagcc tgctacaact gcgctcgcgg ctgctacaac gcctgcctgg tggcgaaccc
     5701 caaccgggcg cacgccgcgt acgggtacgc cggcatgggg cccttccgcg gcggccaggc
     5761 cgagctggtc cgtgttccct tcgcggaggt gaactgcctg aagctccccg ggagccgcac
     5821 gatgagctgg aggacgactt cgtgatgctg gcggacgtct tccccaccgg ctaccacggc
     5881 acggagctgg ccggtgtccg caccggggag ccggtggcgg tctggggggc cggcccggtg
     5941 ggtcagatgg ccgccctcag cgctctgctc cggggcgcca gcgaggtcta cgtggtggac
     6001 ggcgtgccgg agcggctgga gaaggcggcc gagatcgggg cgatccctat cgacttccgg
     6061 gagggctcgc ccagcgagca gatcaagcag caccggcgcg acgaccggct ggtgggaggc
     6121 tcgtggcggc cgggtgagga gaagatggac ggcgtgatgg cggcggtgga cgcggtcggc
     6181 taccaggcca gggacgagga cgacccctcg tgagagatgc ccacgcaggc cctcgaggac
     6241 atcgtcgagg tgctgaacgc gaccggccgg ctggggatcg tgggggtcta catcgctccc
     6301 gatcccgggg cgcccaccga ggctcgcaag caggggcggt tcgagctgcc gctcgccaag
     6361 ctgttcgaga agacgatctc gatcgggatg gggcagtgcc cggtgaagcg ctacaacacg
     6421 ttcctccgcg atcagatcgt ggccggacgg gcccggccga gctacttcgt gagccaacgg
     6481 ccgtcgctgg acgaggcgcc ggaggcgtac gagcggttcg accgccgtgc ccggctacac
     6541 gaaggtggtg ctcaagcccg aggccgcgta gggcaggccc cggcagcggc ggggatcagc
     6601 tcaggccgcc ggcgatccgg tcggcgaagg atcggacctc gtcccagtca cggaagtcgc
     6661 cggtaggcgc tttgacggtc ttgacgatcc gccgttcgag gaagcccaac ctctccgggt
     6721 cgatcgcccc gaagaacacg tggtgctccc tcgccgccac cagccccgcc agcttggcca
     6781 gctgcttgtc ggcgagcccg acctcggtgg ggccctctcc ggtgggtccg ctgctgaaca
     6841 gccacaccgg tagggtgtcc agttcgtccc ggtgccgctc gaggaaggcc gaggcctcct
     6901 tcatccacga gcccatgtag atcgcgctgc ccaccaccac ggcgtcgacc gctccgaggt
     6961 cgtcgcggtc gccgatcggt tcgacctccg tcttgatccc gcgctcccga agccgctcgc
     7021 cgatcgcttc ggcgatcccc tcggtcgcgc cgtgcttgct cgcataggtc accaacaccg
     7081 gcatgctcat caccccttcc catggagcca agcaggtctg ggggcgacgt cgcggggcca
     7141 tggacccggt cggggcggga ccgtcgggcg gcgtccgtcc tggtagggtc gatggcaggg
     7201 caccgatcgc ggttcgccgg taaccaggaa gcgcttgcac gccgtgggat cccgcccgga
     7261 ggctcgggcg ccggagtcgc gacgcacgac caacgaggag gacgccatgc ctgacagtgt
     7321 gggcaagagg aagcgacggg acgtgaccgc caagaaggcg gccgctcggg aagagcgtcg
     7381 gctggcgcgg gcgtcccgca agcgggaccg ggaggccggc ctgatcgagt cgggtccgcc
     7441 catcggtccg gcggagcaga gcgagtggct ccccggccac ggcgaagccg ccgaggcgca
     7501 ggaggaggag gcggcggaag cctccgagtc ctgagcgccg gcgctcgggt catccgccga
     7561 gggctctcga ggacgaggcg ggaggtgaag ggatggcgga ggcggtgcag ccggagaccg
     7621 accgttcggt cggcgtgcgc gagctcggcc acgtggtcct gtacgtgcga gacctgtcca
     7681 ggtcggtgac gttctaccgg gacgtgctcg gctggcggca ggtggtgggg gccgagaccg
     7741 tggggatgcc ggtcgcgatg ttctcggggg gacggacgca ccacgagctg ctgctgatcg
     7801 aggtggggga gggggccacg ccgatcccgg cggggcgccg cgtgggcctg taccacttcg
     7861 ggctcaaggt cggggacacg gacgaggagc tccggaccat gctcacccgt ctggtggatg
     7921 cgggcctgca gctcaccggg acggccgatc acggcatgac ccacagcctg tacctggccg
     7981 atcccgacgg gaacgagatc gagctgtacg tcgacgtgcc cggcgcggac tggaacgatc
     8041 ccgaggtcct gatgtcccga ccccgccccc tccggctctg aacgggttca gcgtgccgcc
     8101 ggacgcggcc ggatggcacc ggcacccagc gccgcagcca ggcacatggc caccgggatc
     8161 accagggcag tgcgcagccc gaaccggtcg gcggtgaatc ccacggccat cggcccggcg
     8221 atcgagccca ggtagccggc cgtcacggcc aggcccagca ccgaccccga ccgcgggcgc
     8281 cgcatcctcg ccgcctcgct gaaggccagg gggaccgccg ggcccacgcc gagccccacc
     8341 agggtgaatg ccgtcagcgc cgccgcggcg ccgcccgcga tgagcgcggt gccgagcgcc
     8401 gcggccgcga ccgccgtacc ggtgcccacg accgtcgaag gctcgatgcg ggcggcgagc
     8461 cgatcccccg ggaagcgcga cgccgccatc ccggcggaga acgcgacgat ccccaccccg
     8521 gccacgcccg gggtggtgcc cgccgaggag cggaggtaga cggcgctcca ctccgcagcc
     8581 accccctcgg tgaggaaggc cgcgaagggg atcaggcaga gcagcacgac cgtgccggtg
     8641 gaccgtggcg acgatcgaac cgacgaggtc gctgggctag gaggcccgcg gtcggccgcg
     8701 ggacggcggc ctggatgctc gttgcccgcc ggcggcagcc atcgcagtag cggccagctc
     8761 cccgccgcca gcaggatcgc caccacggcc aggtgcacct cgatcggcac gcgggcggcc
     8821 acgccggcag aggcgatagc ggctccaacc agcatcgacg cgctccaggc gccgtgcatc
     8881 ccggacatca cccggcgcct cgctcgagcc tccacagccg ccgcctcgag gttcatcgcg
     8941 acatcgagca ggccgctcac caggcccagg agggcgaaga tcccggcgag ccaggccagc
     9001 cggccggcca ggggcaggag ggccagcgcg gccgccagca gggggatccc cgtccggacc
     9061 gcgccgcgcc ccgtccgacg caccacgggc cccgccaacc gggtccccgc caccaacccg
     9121 cccgcgtagg cgccgagcgc cagtcccagg gcgcccgcat ccaggcccga ccgagccttc
     9181 agcgaaggga tccaggggcc gacggtcccc gccaccacgg catgggccga gaaggtcgct
     9241 gccacggcga tgcgctctcg gaccgccggc cgggtgctgt ccacggcccg gaatcatgcc
     9301 cgacccccgg tccttccgtc aggtggaggg gccccgccca gcagatcggg tcggtacagg
     9361 taggcgcggg cggatagctg gtgcatctcc gaccgagcgg tgagcgagat cagatcgctg
     9421 atccagcgct cggccataag gtgccggacc gcgaccgcca cggcgcacaa cccgtcgagc
     9481 acgcaggcgc gcagccagag atgcgtgtcc cgctggcggc tgcagtgggt cagcgccagg
     9541 tccatcgtct cggtggctcc gtccaggtct ccccgcgccg gcttgaggag ggcgaacccg
     9601 cggacgccgt gggcctccct gcagccgtcg tccacccgcg aggtgagccc gaaggcatgc
     9661 tcgaaggcct ccgaggctcg gtccagtctt ctggcccgga tccgtcaggg tggactacgg
     9721 cgcgaactag gagcgcctgg tgcaggtcga gcgtgcgtac gatcccgaca acctgttcta
     9781 cctgaaccag aacatccagc cctgaggggc cccgctcgac gccccaggtc cccacggcga
     9841 tgatggccgg gtggaggtgg acctgagggc ctaccaggca cggatcggct accaggggcc
     9901 ggtaagaccc acggccgact gcctggccga gctgcacctg gcgcacgtgg gctcgatccc
     9961 gttcgagaac ctcgacatcc tcctgggccg gccgatcctc ctggacctcg agagcctgca
    10021 ggccaagctg gtcgaagcgc gccgcggtgg ctactgcttc gagcacaaca cgctgttcgc
    10081 ggcggtgctg gagacgatcg ggttcgacgt gaccaggctc gccgcgcggg tcggccacga
    10141 ggcacgtgct cccgcgcccc ggacccacat ggtcctgggg gtggaggccg aggggttcca
    10201 gtggctggcc gatgtcgggt tcggcggcgc ctcgatcctg cggccgctgc cgttcgagcc
    10261 cgggccggtc gtcgagcagt tcgggtggcg gttccggctg gtggacgtcg atgggctccg
    10321 ggtggtccag gcgctccgg
//
LOCUS       DASQQQ010000098         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_172440, whole genome shotgun sequence.
ACCESSION   DASQQQ010000098 DASQQQ010000000
VERSION     DASQQQ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172440"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /locus_tag="VE646_04050"
     CDS             <1..1297
                     /locus_tag="VE646_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZD79199.1"
                     /translation="ALTVGVGLGACGTGEAGEAPAASTGRVERGDLRITAEATGSVEP
                     VRQVQVKSKASGEVLELYADVGDEVEPGTLLAEVDPRDVRNAYDQAQADLAVAKESRR
                     IAAAQLERQQELAEAGFITEQELESARLQDANAEAGLIKSQTNLELAQLRLADVTIRA
                     PLAGTILTRSVEEGSVIQSASQNVSGGSVLFEMADLDSMQVRTLVDETDMGSLRAGME
                     ATVTVEAYPDRVFRGAVERIEPQAVVQQSVTMFPVIVRLDNRSGLLRPGMNAEVEILI
                     DQASDVLLVPNNAIVNLGDVGPAAMVLGLDVEKMDLGPFQEAARDGSRGGNDRPAIGS
                     GLMEVPASQQPAGAAEEARAGRKSVVFVKADSTASAQPRLVEIGLNDWDRTEVRSGLQ
                     EGEMVALIGAVALQAQQNEFMERMRSRGPDGPFGGGRGR"
     gene            1320..2540
                     /locus_tag="VE646_04055"
     CDS             1320..2540
                     /locus_tag="VE646_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD79200.1"
                     /translation="MLIKEIIMVALAAIRANALRSFLTTLGIIIGVAAVIAMVALGGG
                     AQRRVEDQIQRMGTNVLTIRAGQGMFGGIRGQGGVRLSVEDAEALRGGTSGGLLTVAP
                     EASSRQQLSYLRWNSNNEIVGTWPEYFEIYEHKLVQGRFFDHGEVQGRRRVAVLGFNV
                     PEEDLGTPVPLLVGKTIQIGGQPFQVIGVLAEKGEMGRSNPDGSVYIPVSTAIYRVFG
                     GRERLGSIYAKTPTPAEMDQAFAEIDRILRREHRIPPGEEADFSISNAADLLESFSET
                     SKVFGYLLAGIAGISLLVGGIGIMNIMLVSVTERTREIGVRKAMGATKKAILFQFLIE
                     ALVLCVLGGILGVVAGWGSAHLITRIAEWPTAVSYLAVMTALGFSAGIGLFFGIWPAQ
                     RAARLDPIDALRYE"
     gene            2704..3288
                     /locus_tag="VE646_04060"
     CDS             2704..3288
                     /locus_tag="VE646_04060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZD79201.1"
                     /translation="MAEQVQTTAPSETDGQLVERVRAGDGRAFDRLVRRHLRVAHAVA
                     RSKLSGNEDDADDVCQEAFMTALERIEDCRDPARFRPWLLSIVRNKAHNYRAYLAVRE
                     AEPLDVVEPAAGTDDVGARVERHELKDDLAQALAELTELQRTVFVRFDLEGWGHGEIA
                     EALGVSRGASRFHLHAARRALRKRLSAHRLAWSR"
BASE COUNT          515 a         1068 c         1259 g          481 t
ORIGIN      
        1 cgctctcacc gtgggcgttg gactcggcgc gtgcggaacc ggggaggccg gggaggcgcc
       61 ggcggcgagc acgggtcggg tggagcgggg cgacctgcgg atcaccgccg aggccaccgg
      121 ctcggtggag ccggtgcggc aggtgcaggt gaagtccaag gccagcggcg aggttctcga
      181 gctatacgcc gacgtgggcg acgaggtgga gcccggcacg ctcctggccg aggtggaccc
      241 ccgggacgtg cgcaacgcct acgaccaggc ccaggccgac ctggccgtcg ccaaggagag
      301 ccggaggatc gcagcggccc agctcgagcg ccagcaggag ctcgccgagg cgggcttcat
      361 caccgagcag gagctggaga gcgcgcggct tcaggacgcc aacgccgagg cgggcctcat
      421 caagtcccag accaacctgg agctggccca gctccggctt gccgacgtga cgatccgcgc
      481 cccgctggcc gggacgatcc tcacgcgcag cgtggaggag gggtcggtca tccagtcggc
      541 gtcgcagaac gtgagcggcg gctcggtcct gttcgagatg gccgacctgg actccatgca
      601 ggtgcgcacc ctggtggacg agaccgacat gggctcgctg cgggcgggga tggaggcgac
      661 ggtgaccgta gaggcctatc cggaccgcgt cttccggggc gcggtggagc ggatcgagcc
      721 gcaggcggtg gtgcagcaga gcgtcaccat gttcccggtg atcgtgcggc tggacaaccg
      781 ttcggggctg ctgaggccgg ggatgaacgc cgaggtggag atcctcatcg accaggcgag
      841 cgatgtcctg ctggtgccca acaacgccat cgtgaacctc ggcgacgtgg ggcctgcggc
      901 catggtgctg gggctcgacg tcgagaagat ggacctcggc ccgttccagg aggccgctcg
      961 tgacggaagc cggggaggga acgaccgccc ggcgatcggc tccggtctga tggaggtccc
     1021 ggcgagccag cagcccgcag gagccgcgga ggaagcccgg gcgggccgga agtcggtggt
     1081 gttcgtcaag gccgactcca cggcctccgc gcagccgcgg ctggtggaga tcggcctgaa
     1141 cgactgggac cggaccgagg tgaggtcagg cctccaggag ggggagatgg tcgccctcat
     1201 cggcgccgtg gcgctccagg cccagcagaa cgagttcatg gagcgcatgc ggagccgcgg
     1261 gcccgacggc ccgttcggtg gcgggcgggg tcgctgagcg tcctcatcgg agaccgacga
     1321 tgctgatcaa ggagatcatc atggtggccc tggccgccat ccgcgccaac gcgctgcgct
     1381 cgttcctcac gacgctgggc atcatcatcg gtgtggcggc ggtcatcgcc atggtggctc
     1441 tcggcggcgg cgcccagcgc cgggtcgagg accagatcca gcgcatgggc accaacgtcc
     1501 tcaccatccg ggcgggtcag ggcatgttcg ggggcatccg gggacagggt ggcgtccgac
     1561 tgagcgtgga ggacgccgag gccctgcggg gcgggacttc cggggggctg ctcaccgtgg
     1621 cgcccgaggc gtcgtcccgg cagcagcttt cctaccttcg ctggaactcg aacaacgaga
     1681 tcgtgggcac ctggcccgag tacttcgaga tctacgagca caagctcgta cagggacgct
     1741 tcttcgatca cggagaggtc cagggccgac ggcgtgtggc cgtcctcggc ttcaacgtgc
     1801 ccgaggagga tctcgggacg cccgtgcccc tcctcgtcgg gaagacgatc cagatcgggg
     1861 ggcagccgtt ccaggtcatc ggtgtgctcg ccgagaaagg ggagatgggc cgctccaacc
     1921 ccgacgggag cgtctacatc cccgtctcga cggcgatcta ccgggtgttc gggggtcggg
     1981 agcgtctcgg ctcgatctac gccaagacgc ccacccccgc cgagatggat caggccttcg
     2041 ccgagatcga ccggatcctc cggcgggagc atcgcatccc tccgggcgag gaggccgact
     2101 tcagcatcag caacgccgcc gacctcctgg agtcgttcag cgagaccagc aaggtgttcg
     2161 ggtacctgct ggctggcatc gccgggatca gcctcctggt ggggggcatc ggcatcatga
     2221 acatcatgct ggtgtcggtc accgagcgga cccgggagat cggcgtgcgc aaggccatgg
     2281 gagccacgaa gaaggccatc ctgttccagt tcctcatcga ggccctggtc ctctgcgtcc
     2341 tgggaggcat cctgggcgtg gtggccgggt gggggagcgc gcacctcatc acccggatcg
     2401 ccgaatggcc caccgccgtc tcgtacctgg cggtgatgac ggccctgggc ttttcggcgg
     2461 ggatcggcct ctttttcggc atctggccag ctcagcgagc tgcgaggctc gatccgatcg
     2521 atgcgctgag gtacgagtga tgagatgggc gacgaccggt cgggaaggct cgaccccgcc
     2581 ggcgccagga ggtatgcatt gtccggagcg ctgacgttgc cctggggccg gtggccggtc
     2641 ccacgaaggg ctccggccgg actcgtccgg ccggacccga gtcgagtcca gccgggatat
     2701 gcggtggccg aacaggttca gacgaccgcg ccgtccgaga ccgacggcca gctcgtggag
     2761 cgggtgcgtg ccggggatgg gcgggctttc gaccgcctgg tccgccgtca cctgcgggtg
     2821 gcccacgcgg tggcccgctc gaagctgagc gggaacgagg acgatgcgga cgacgtctgc
     2881 caggaggctt tcatgacggc tctggaacgc atcgaggact gtcgggaccc ggcgcgcttt
     2941 cggccctggc tgctctccat cgtgaggaac aaggcgcaca actaccgggc gtacctggca
     3001 gtccgggagg cggagccgct ggacgttgtg gagcctgcgg ccggcacgga cgacgtcggc
     3061 gcccgggtcg agcgccacga gctgaaggac gacctcgccc aggcgctcgc ggagctcacc
     3121 gagctgcaac gcacggtgtt cgtccgcttc gatctggagg gatggggcca cggcgagatc
     3181 gcagaggcgc tgggcgtttc gcggggagcc tcccgcttcc acctgcacgc ggcacgccgc
     3241 gcgctccgga agcggctctc cgcccaccgg cttgcatgga gtcgatgatg gaagctgacc
     3301 gaggcgggag gcgctcccgg gtg
//
LOCUS       DASQQQ010000099         3601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_116537, whole genome shotgun sequence.
ACCESSION   DASQQQ010000099 DASQQQ010000000
VERSION     DASQQQ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116537"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VE646_04065"
     CDS             complement(<1..1010)
                     /locus_tag="VE646_04065"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZD79202.1"
                     /translation="MRIVLSWLREICPTDLGVEDLAELLTRKGAEVEGVERPWDRLRG
                     VLVARVLEVRDHPNSDKLCLARVQTGSGEQEVVVGVRNMAAGDLVPLAPPGATVPALP
                     EPLAARPIRGMVSNGMLCSPMELGIAPSHEGILVLPDDLEPGQDVRSVLGLDDAVLDV
                     EVTPNRPDFLSVLGIAREVSAATGVPIVPPPTSVREEEGKAEDAASLEVLDLDRCPRY
                     LARILTGVRHAPSPIKIQARLTAAGMRPISGAVDATNYAMLEIGQPLHPFDLALLRGP
                     GVVVRRAAEGERLVTLDDVERAFTGDDLLICDPERPVAVAGVMGGEVAEVSDATTEVL
                     LE"
     gene            complement(1007..2053)
                     /gene="pheS"
                     /locus_tag="VE646_04070"
     CDS             complement(1007..2053)
                     /gene="pheS"
                     /locus_tag="VE646_04070"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HZD79203.1"
                     /translation="MDPQEALRVLEQERRRGLERFAEAGSTDELEAAKVAVLGRRSPW
                     SQVQRSLGGLGVEEKRRLGRLSNEVKAELEEALERRRAVLVQSDETRLLQADRVDVTL
                     PGRRPREGSLHPLTLIEEEIVEVFTRMGYRVEEGPEIEDDWHNFEALNIPPDHPARTM
                     KDSLYVDVPGLLLRTETSAVQIRTMERQAPPVFIVAPGRVYRREAVDATHLSVFHQIE
                     GLAVDEGLSFSDMKGTLEAFARAMFGPERRVRLNPDYFPFVEPGAQVAVSCFICGGLS
                     CPTCGNGWIELLGAGMVHPRVLENCGIDPERYTGFAFGLGIERVAMVRYGIHDMRSFV
                     DGDVRFLQQFQGVA"
     gene            2444..3007
                     /locus_tag="VE646_04075"
     CDS             2444..3007
                     /locus_tag="VE646_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZD79204.1"
                     /translation="MRKLIYGMNLTLDGYVAAPGDDLGWSGPSDELFQWWLDQERAIG
                     LLLYGRKLWEVMSSYWPTGDQQPDATPAEIEFARNWRDTPKVVFSSAMEKVDWNTRLV
                     TGDAVEEITRLKAEDGEPMRVGGATLAGAAMRAGLIDEYTIVTHPVLVGGGTPFFTAL
                     DSWVNLNLLETRTFPGGVVLTRYETRR"
     gene            complement(3295..>3601)
                     /locus_tag="VE646_04080"
     CDS             complement(3295..>3601)
                     /locus_tag="VE646_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79205.1"
                     /translation="LQLILEAFMEAEVWWASEGPVVVSGGVDEGRLVLEVFRAGTELD
                     PEGAGALFEPRPPGTGSGSKIGLFVARGVAEAQGGSATVHVEDGLRFRLEVPAVPEP"
BASE COUNT          576 a         1296 c         1226 g          503 t
ORIGIN      
        1 ctctccagca gcacctcggt ggtcgcgtcg gagacctcgg ccacctcgcc ccccatcacg
       61 ccggccacgg ccaccggtcg ctccgggtcg cagatcagca ggtcgtcacc ggtgaaggcg
      121 cgctcgacat cgtccagggt caccaggcgc tcgccctccg cggcgcggcg gaccacgacc
      181 cccgggcccc gcagcagcgc caggtcgaag gggtgcagcg gctggccgat ctcgagcatc
      241 gcgtagttcg tggcatccac ggccccggag atgggccgca tcccggcggc ggtgagccgg
      301 gcctggatct tgatgggcga cggcgcgtgt cggacgcccg tcaggatccg cgccaggtac
      361 cgtgggcagc ggtccagatc caggacctcc aggctcgcgg catcctcggc tttgccctcc
      421 tcctcccgca cgctcgtggg cggcgggacg atcgggaccc ccgtcgccgc cgagacctcc
      481 cgagcgatcc ccagcaccga gaggaagtcg ggccggttcg gtgtgacctc cacgtcgagc
      541 acggcgtcgt ccaggcccag caccgagcgc acgtcctggc cgggctcgag gtcatccggc
      601 agcaccagga tgccctcgtg gctgggggcg atcccgagct ccatgggcga gcagagcatg
      661 ccgttggaga ccatgccccg gatgggccga gcggcgagcg gctcgggcag ggcgggcacc
      721 gtggccccgg gcggggccag ggggaccagg tcgcccgccg ccatgttgcg gacgcccacc
      781 acgacctcct gctcgcccga gccggtctgc accctggcca ggcacagctt gtccgagttc
      841 gggtgatcgc ggacctccag cacgcgcgcc accagcacgc ctcggaggcg atcccagggg
      901 cgctcgacgc cctccacctc ggcccccttg cgcgtgagca actcggccag atcctccacg
      961 ccgagatccg tggggcagat ctcgcggagc caggacagga cgatcctcac gcgaccccct
     1021 ggaactgctg gaggaagcgc acgtcaccgt ccacgaacga ccgcatgtcg tggatgccgt
     1081 accggaccat ggccacccgc tcgatcccca gaccgaaggc gaaccccgtg tagcgctcgg
     1141 ggtcgatccc gcagttctcc aggacgcgcg gatgcaccat cccggcacca agcagttcga
     1201 tccaaccgtt cccgcacgtg ggacagctca ggcccccgca gatgaagcac gagacggcca
     1261 cctgagcccc cggctcgacg aaggggaagt agtccgggtt caggcggaca cggcgctcgg
     1321 gaccgaacat cgccctggcg aacgcctcga gcgtcccctt catgtcggag aaggacaggc
     1381 cctcgtccac cgcgagcccc tcgatctggt ggaacacgga caggtgggtg gcgtccaccg
     1441 cctcccgtcg gtacacgcgg ccgggcgcca cgatgaacac gggcggtgcc tgtcgctcca
     1501 tcgtgcggat ctgcaccgcc gaggtctctg tgcgtagcag caggcccgga acgtcgacat
     1561 agagcgagtc cttcatggtg cgagccgggt gatcgggtgg gatgttcaac gcctcgaagt
     1621 tgtgccagtc gtcttcgatc tcggggccct cctcgacccg gtaccccatc cgggtgaaga
     1681 cctcgacgat ctcctcctcg atcagggtca gggggtgcag cgacccctcg cgggggcgcc
     1741 ggccgggaag cgtgacgtcc actcggtccg cctgcagcag ccgcgtctcg tccgactgga
     1801 ccaggaccgc ccgtcgccgc tcgagggcct cctcgagctc ggccttgacc tcgttggaga
     1861 gccgtcccag ccgccgcttc tcctcgacgc ccaacccgcc gagcgagcgc tgcacctgcg
     1921 accaggggga acggcgcccg agcacggcca ccttggccgc ctccagctca tcggtggatc
     1981 cggcctccgc gaagcgctcg aggccgcggc ggcgctcctg ctccaggacg cggagggctt
     2041 cctgcggatc catgggtgcc agagtctagc aagcccccct cccaggaccg ggcggggtgt
     2101 tccccagggc gccgtgggcc gctcgtggtt gtacgtgttg aggaagtccg cgagcgcggc
     2161 ggtgcgctct tcgtcgctgg cataagggac ggacgtagag ccactcgatc ttcatcgtca
     2221 gcacactcct gaagccagaa ccccgcagcg gtggaggctc gctcgccggg gtggacttcg
     2281 gcgtaggcca tgcggctgtg gtcctcgaca gccgcatgga tgtagccgtg tcgcgacggg
     2341 tcgcgaacca ccgacctggg tactcgcgcc tcgtcggtgc cacgtcgggc gtgcgctgaa
     2401 ccggtctaag cttccgagac cggccgaccg aaggcatggt gcgatgcgga agctgatcta
     2461 cggcatgaac ctgacactgg atggctacgt cgccgcgccc ggcgacgacc tcgggtggag
     2521 tgggccgagc gacgagctgt ttcagtggtg gctcgatcag gagcgggcga tcggcctgtt
     2581 gctgtatggg cgcaagctgt gggaggtcat gagttcctac tggccgaccg gcgaccagca
     2641 gcccgatgcc accccggcgg agatcgagtt cgcgcgcaac tggcgggaca cgccgaaggt
     2701 ggtgttctcc tcggcgatgg agaaggtcga ctggaacacc cgcctggtca ccggcgacgc
     2761 ggtcgaggag atcacccggc tcaaggctga ggacggcgag ccgatgaggg ttggtggtgc
     2821 aacgctcgcc ggggcggcca tgcgggccgg gctgatcgac gagtacacga tcgtcaccca
     2881 tccggtcctg gtgggcggcg gcacgccgtt cttcaccgcg ctggatagct gggtgaacct
     2941 gaacctgctg gagacgcgga cgtttcccgg cggggtggtt ctgaccaggt acgagacgag
     3001 gcgctaagcg cgttccctcc acgcggccaa ggacgtgacg aggcagtgat gggcccaccg
     3061 ggcttgatca cgggatcggt tccacggaag ttctggaccc accacgatgg tcgaagacgg
     3121 ccgccggcag gtcgctcgac acgaccggct caccggccga ctccaagccc acctcgaact
     3181 cgaccaccac ctgtaggcac tgatccgcaa agccgcgttg cccggcgcca cagcactcca
     3241 acgagcccgc gccgcggcgg gaactcgtgt caacagcgtc cagagggaac acagctaggg
     3301 ctccgggacc gcggggacct ccagccggaa ccgcaggccg tcctcgacgt gcaccgtggc
     3361 ggagccgccc tgggcctcgg ccacgccccg ggccacgaac aggccgatct tgctgcccga
     3421 gccggtgccc gggggacgag gctcgaacag ggcaccagcc ccctcggggt cgagctcggt
     3481 accggctcga aacacctcca gcaccaaacg gccctcgtcc accccgcccg agaccaccac
     3541 cggaccctcc gatgcccacc agacctcggc ctccatgaag gcctccagga tcagctggag
     3601 c
//
LOCUS       DASQQQ010000100         9356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_63209, whole genome shotgun sequence.
ACCESSION   DASQQQ010000100 DASQQQ010000000
VERSION     DASQQQ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9356
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63209"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VE646_04085"
     CDS             complement(<1..243)
                     /locus_tag="VE646_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HZD79206.1"
                     /translation="MTEHPSLVVCIGGSAGGHDALRELFDRVDHDVDAVFLVVLHIAA
                     AGQSVLPRIVARATDLQVLPATDGAPLVRGRVYVAVA"
     gene            complement(308..2281)
                     /gene="ftsH"
                     /locus_tag="VE646_04090"
     CDS             complement(308..2281)
                     /gene="ftsH"
                     /locus_tag="VE646_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009108927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZD79207.1"
                     /translation="MRPERSSDPQGGADGRPPWSRSIVWILVGVAALFLLFGPSLLGS
                     GGRSIPFSQFLDLVQNGSVTQVTLSQGQVQGMYQPSGGGSSQQGGSSQQTAFTSTLPT
                     NYNVNQLIDQLTKQGVEVNATRPSGLVQFFVIWGLPLLLLVGGYFLFRRMLRNRMGGD
                     GGSMGGGPFGFGRSKAKLYEKSKLRTTFQDVAGLDEVRAEVAELVDYLKTPGRFRRLG
                     AKIPKGVLLVGPPGTGKTLLARAVAGEADVPFFSVSASTFVEMLVGAGAARVRDLFER
                     ARRRAPAIVFIDEIDAIGQARAGRAGSQLGAHQEREQTLQQLLSEMDGFEPNSGVIIV
                     AATNRPEVLDQALLRPGRFDRQIVVHLPDVRGRHEILKLHARGVALGLDADLETLARR
                     TPGFSGAQLANVINEGALLAGRRGKDHVAMSDLEDAIDRVVAGLERRSQIISEEERRM
                     IACHEAGHAVVGHSARHADRIHRISIVPRGIGALGFTQQLPDEDRYLVSEPELHDRLA
                     TLLGGRCAEQLVFGFVTSGAQDDLRKASDIARRMVTEFGMSETVGPVVVSEPDSQFLQ
                     SALMRRPDLSDETEQAIDREVRNLIERAKGQAETMLSEHREEHRRLMEALLEEETLEG
                     PRIEELIGDGSRDGQTSDRRNASGAEGPTQRSA"
     gene            complement(2347..2568)
                     /locus_tag="VE646_04095"
     CDS             complement(2347..2568)
                     /locus_tag="VE646_04095"
                     /inference="COORDINATES: protein motif:HMM:NF040017.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6186 family protein"
                     /protein_id="HZD79208.1"
                     /translation="MGPYEVIGTVGWVVILGAIAAWEVATLVHHDPRTPTMGDVVRFF
                     LRPTVGRWLLFVVWLWLGWHFFIRTTVGA"
     gene            2892..3218
                     /locus_tag="VE646_04100"
     CDS             2892..3218
                     /locus_tag="VE646_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79209.1"
                     /translation="MSLPAGRWALYAGWIWLGWDLLPTEGAPLSGRPWASRVRRGLVI
                     AAVLGYAWWAAFLRSEVRWVDVLVPAAGLLAIALFGHLRRSTPISSMARATRDWIELQ
                     LRRARR"
     gene            3215..4870
                     /locus_tag="VE646_04105"
     CDS             3215..4870
                     /locus_tag="VE646_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZD79210.1"
                     /translation="MRLRSHLPFRSHGGLVALTTGLPLAEGLVLRWSGMAAAQPLAPQ
                     ITAPEPFGIFHDLRWVSVFHDAWWSFALELLLLVAFRAGLMTLLVRLAWPGDRPRPPL
                     RPMLIHNAIFTAAVLVLLTPSEALIFASAAAPVSWIFLAAWPLALLVAVLVFPGPVRH
                     DWWRWRAPARSVGWVFLTFVVLTAGGALVASSPPGLAELAIVATGLFDAWAWVRIVRG
                     LIEPGASAIRLPQPSISLALMAAVVLGMTALTFGLLRDVSRLNQSASRPASRSGRPVL
                     LVTGFGSSWSGRPDPLLGSDFDQRRFSYAGMGPNLDPLPFGPQATQRPLPVLVREMAR
                     QVRAFAEQTGQQVSIVAESEGTVVAKTYLATHPGAPVSRLIMLSPLVDPGRVYFPPPG
                     REGWGVASGWEVRGISAVIRWLSSLQVAPDVPFFRDLVNEAPALQRILRCPLAGVRQI
                     AFEPLADAVTGLPPTEGSGAVPRVVIPAFHGGMLESASVQRSVDVVLSGGTLPSFRAW
                     EVLDRAVRAVASPWQSPSLPATLPTAWDHTRLPDPVCAPVGST"
     gene            5043..6887
                     /locus_tag="VE646_04110"
     CDS             5043..6887
                     /locus_tag="VE646_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HZD79211.1"
                     /translation="MATTKRTGPRADTYLPIEEYGMIGDCHSAALVSRTGSIDWCCLP
                     RFDAGSAFGRLLDRDRGGHCSIRPRARSVHTRRRYLENTLVLETVVRAGGAEARMIDG
                     LTMPSGPGRDREILRAVDGLRGRMSFEIEVEPRFDCGRLRPWITQLGVHCYAAVGGDD
                     CLIVWSDADLEPVGEDALRGRFEVGEGQRVRLAIRYIRPHELDGGRGQDFDPTDLDDR
                     LERTIEWWREWASHGSMDHPHGSAALRSAITLKAMTYAPTGAMIAAATTSLPESPQGA
                     RTWDYRYSWIRDSAFAVRSLARLGFEEEADGFRRFVERSAAGHAEDLRILFGVGGERH
                     LMESTVPELEGYRGAKPVRIGNGASTQLQLDVYGHVVELAWRWHQRGHSPDDDYWRFL
                     MDIVETAAARWEERDRGIWEVRGRRQHFVHSKVMCWAALDRGIRLATECGRRAPLRRW
                     RQVRREIRSAVEQHGYDRKRGVFTRSFGTKSMDAALLLLPQVDFVPVDDERMMRTVDE
                     VRKDLGQDGLLLRYRVSDGIDAPEGVFLPASFWAVECLAGQGRLEEAEALFERAVAAA
                     NDLGLFSEEYDPGTYHMLGNFPQALTHMSHIGASVAIAAAANQISPPL"
     gene            6909..7889
                     /locus_tag="VE646_04115"
     CDS             6909..7889
                     /locus_tag="VE646_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZD79212.1"
                     /translation="MEIRPLGSSGLSVTPIGLGLAALGRPAYIDLGRERDLGPERSPE
                     AMRARCHAVLDAAFDAGIRYVDAARSYGDAERFLSTWLAGRGSTVAELTVGSKWGYTY
                     VADWRMDADVHEVKDHSLQTLRRQVGESRALLGSRLALYQIHSATLESGVLEDRGVLE
                     ELVRLRGDGLAIGLTTSGARQGETVRRALQVRVAGERPFDVVQATWNPLEPSAGPALA
                     EAKEAGWGVIVKEAVANGRLVPGAPDLPRELDSVAERCGATVDQVAIAAALENPWADM
                     VLSGAVTEGQVRSNAGAAEIGLEPGDLDALAGLAEPPERYWAARSALPWR"
     gene            8093..8167
                     /locus_tag="VE646_04120"
     tRNA            8093..8167
                     /locus_tag="VE646_04120"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8125..8127,aa:Val,seq:cac)
     gene            complement(8424..9356)
                     /locus_tag="VE646_04125"
                     /pseudo
     CDS             complement(8424..9356)
                     /locus_tag="VE646_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418914.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         1238 a         3256 c         3421 g         1441 t
ORIGIN      
        1 ggcgaccgcc acgtagaccc ggccccggac gagcggcgcg ccgtccgtgg cggggagcac
       61 ctggaggtcc gtggcacgag ccacgatccg gggcagcacg ctctggccgg ccgccgcgat
      121 gtggagcacc accaggaaca ctgcgtccac gtcgtggtcg acccggtcga acagttctcg
      181 gagcgcgtcg tgtccgccgg cggagcctcc gatgcacacg acgagcgaag gatgctcggt
      241 cacggcgtgg ctcctccccc tcgaacctta cccgtccccg agggtgggcg gaagccccgt
      301 gggcacctca cgcgctccgc tgcgtcggtc cctcggcccc cgaggcgttc cgccggtccg
      361 aggtctggcc atcgcgcgat ccgtccccga tcagctcctc gatcctgggg ccctcgagcg
      421 tctcctcctc caggagggcc tccatcaacc ggcggtgctc ctcgcggtgc tcggacagca
      481 tggtttcggc ttgccccttg gcccgctcga tcaggttccg gacctcgcga tcgatcgcct
      541 gctcggtctc gtcggacagg tcggggcgac gcatcagcgc gctctgcagg aactgggagt
      601 cgggctcgga aaccaccacc ggccccactg tctcggacat cccgaactcg gtcaccatgc
      661 gccgagcgat gtcggaggcc ttgcgcaggt cgtcctgcgc cccgctggtc acgaacccga
      721 acaccagctg ctcggcgcag cgaccgccca gcagggtggc caggcggtcg tgcagctccg
      781 gctcggagac caggtaccga tcctcatcgg gcagctgctg ggtaaacccc agggcgccga
      841 tgccgcgggg tacgatcgag atgcggtgga tccggtccgc gtgtcgggcc gagtgaccca
      901 ccaccgcgtg gccggcctcg tgacacgcga tcatgcggcg ctcctcctcg gagatgatct
      961 gggaccgccg ctccagcccg gccaccacgc ggtcgatcgc gtcctccagg tcggacatcg
     1021 ccacgtggtc cttgccccgg cggccggcga ggagggcgcc ctcgttgatt acgttggcga
     1081 gctgcgcacc ggagaacccc ggggtccgcc gggccagcgt ctccaggtcg gcgtcgagac
     1141 cgagggccac accccgggcg tgcagcttca ggatctcgtg ccggccccgc acgtcgggca
     1201 ggtgaaccac gatctgccgg tcgaaccggc ccggtcggag cagggcctgg tccagcacct
     1261 cgggacggtt cgtggccgcc acgatgatga ccccgctgtt cggctcgaac ccgtccatct
     1321 ccgaaaggag ctgctggagc gtctgctcgc gctcctgatg ggcgccgagc tgcgatcccg
     1381 cgcgccccgc ccgcgcctgg ccgatcgcgt cgatctcgtc gatgaacacg atggccggcg
     1441 cgcgccggcg ggcgcgctcg aacaggtcgc ggacccgagc ggctccggcg cccaccaaca
     1501 tctccacgaa ggttgacgcc gagaccgaga agaagggcac gtccgcctcg ccggccacgg
     1561 cccgagccag caacgtcttg ccggtcccgg gcgggcccac cagcagcacg cccttgggga
     1621 tcttcgcgcc cagcctgcgg aaccgccccg gggtcttcag gtagtcgacg agctcggcga
     1681 cctcggcccg aacctcgtcc agcccggcca catcctggaa cgtcgtgcgg agcttgctct
     1741 tctcgtagag cttggccttg ctccggccga agccgaaggg accgccgccc atggagccac
     1801 cgtcgccgcc catgcggttg cggagcatcc gccggaacag gaagtagccg cccaccagca
     1861 gcagcagggg gagcccccag atcacgaaga actggaccag gccgctcggt ctggtcgcgt
     1921 tcacttcgac cccctgcttg gtgagctggt cgatcagctg gttgacgttg tagttggtcg
     1981 gcagcgtgct ggtgaaggcc gtctgctgcg acgagcctcc ctgctgcgac gagccaccac
     2041 cggacggctg gtacatgccc tggacctggc cctgcgacag ggtgacctgc gtgacgctcc
     2101 cgttctggac caggtcgagg aactgggaga aggggatcga gcgaccgccg ctgcccagca
     2161 ggctggggcc gaacagcagg aacagcgcgg ccaccccgac caggatccag acgatcgatc
     2221 ggctccaggg gggtcgcccg tcggcgcccc cctgggggtc ggaggagcgt tcaggacgca
     2281 tcgggcgcac agggtccgtc ggggatcacc gcccctggct tccccgccgg agcacgtggc
     2341 gaaacgctac gcgccgacgg tggtgcggat gaagaagtgc catcccaacc acaaccagac
     2401 cacgaacagc agccaccgtc ccaccgtggg ccggaggaag aagcggacga cgtcgcccat
     2461 cgtcggggtc cgcgggtcgt ggtgcacgag ggtcgccacc tcccaggccg cgatcgcgcc
     2521 caggatcacc acccacccaa cggtgccgat gacctcgtac ggacccatag cccgatcgtg
     2581 ccccggggcg ccgcgccgca tgcggcaagg cggttcggac ccgaaccggt cccggcgggt
     2641 ataggagcgg cgtggtcgcc atgcctcctc cctccgaccg tgggcggccg ggtccgtcgc
     2701 gacccgtacg ggccgcgccc cgcggccgga cgcccgagcg acgcgcaccg ggcccacgcc
     2761 cgcacgggcc ggcccgctcg gccgtactgg cctggtcggt cctgttcgca ctgctggtgg
     2821 gctgggagtt caccggcctg gcgttcggcg gccgggggtg gcctacgttc agcggggtcg
     2881 tacggttctt catgagcttg cctgcgggac gatgggcgct gtacgcggga tggatctggt
     2941 tgggatggga cctgctgccg accgagggcg ctccgctctc gggccgtccc tgggcgtcgc
     3001 gcgtccggcg cgggctcgtg atcgcggctg tgctgggata cgcgtggtgg gcagcgttcc
     3061 tgcggagtga ggtgcgatgg gtggacgtgc tcgtgccggc ggcggggctc ctggcgatcg
     3121 ccctgttcgg gcacctgcgc cggtcgacgc ccatctcctc gatggcgcgg gcgacccggg
     3181 actggatcga gctccagctc cgccgagctc gacgatgagg ctgcgctcgc acctgccctt
     3241 ccgctcgcac ggcggcctgg tggcgctcac caccgggctc ccgctggcgg agggcctggt
     3301 gttgcggtgg agcggcatgg cggcagcgca gccgttggca ccgcagatca cggcgcccga
     3361 gcccttcggc atcttccacg atctgcgctg ggtgagcgtc ttccacgatg cctggtggag
     3421 cttcgctctg gagctgctgc tcctggtcgc gttccgggcc ggtctgatga cgctgctcgt
     3481 tcggctggcc tggccagggg atcggccacg ccctccattg cgaccgatgc tcatccacaa
     3541 cgcgatcttc acggcggcgg tgctggtgct cctcacgccc tccgaggctc tgatcttcgc
     3601 gtcggcggcg gcccccgtgt cgtggatctt cctggccgcc tggccgctcg cgctcctggt
     3661 cgccgtgctg gtcttccccg gaccggtccg ccacgactgg tggaggtggc gggcgcccgc
     3721 ccgatccgtg ggatgggtgt tcctcacgtt cgtggtactc accgcgggcg gggcgcttgt
     3781 ggcatccagc ccccccggcc tggccgagct ggcgatcgtt gcgaccggcc tgttcgacgc
     3841 ctgggcctgg gtccggatcg tgcgagggct gatcgagccg ggagcctcgg cgatccgcct
     3901 gccgcagccc tccatcagcc tggccctgat ggccgcggtc gtcctgggga tgaccgccct
     3961 caccttcggg ctgctacggg acgtctcacg cctgaaccag agcgcctcca ggcccgcctc
     4021 caggtccggc cggcccgtgc tgctggtgac cgggttcggg tcgagctgga gcggccgccc
     4081 ggatccgttg ctcggctcgg acttcgacca gcggcggttc tcgtacgcgg ggatgggtcc
     4141 gaacctcgat cccctgccct tcggtcccca ggccacccag cgaccactac cggtcctggt
     4201 gcgcgagatg gcccgccagg tgcgggcctt cgccgaacag accgggcagc aggtgagcat
     4261 cgtggccgag agcgagggca cggtggtcgc gaagacgtac ctggccacgc atcccggagc
     4321 gcccgtgtcc aggctgatca tgctcagccc gctggtggat ccgggccgcg tctacttccc
     4381 cccgcccgga cgggagggat ggggcgtggc ctcgggctgg gaggtccggg ggatctcggc
     4441 ggtgatccgg tggctgtcct cgctccaggt ggcgcccgac gtcccgttct tccgggacct
     4501 ggtgaacgag gccccagccc tgcagcggat cctccgctgt cccctggcgg gggtgcgcca
     4561 gatcgcgttc gagccgctgg cggatgccgt gaccggcctt ccgcccaccg agggatccgg
     4621 cgccgtcccc cgcgtggtga tccccgcgtt ccacggcggg atgctcgaga gcgcctcggt
     4681 ccagcgctcg gtggacgtgg tgctctccgg gggcacgctc ccgtcgttcc gggcgtggga
     4741 ggtcctggac cgggcggtcc gggccgtggc ctccccctgg cagagcccca gcctgccggc
     4801 gacccttccg accgcctggg accacacccg gttgcccgat cccgtgtgcg ccccggtcgg
     4861 gtccacctga ggctcgatgc ccggagcttc cgggaccccg ccccgtcggg cgcggggtgg
     4921 gcgaccaagc tgggcgcgtc gtccggagcg cggggccgcc tcagccacga cctccgtcgt
     4981 ttgctgcggc accactgcgg ctacatatcg gacgacctcg gccgcaaggg ttcggtgagc
     5041 gcgtggcgac gacgaagagg acggggccca gggccgacac ctacctcccc atcgaggagt
     5101 acgggatgat cggcgactgc cactcggcag cgctggtctc tcggaccggt tcgatcgact
     5161 ggtgctgcct tccccggttc gatgccggga gcgccttcgg gcggctgctg gaccgggacc
     5221 gcggcgggca ctgctcgatc cggcctcgcg cccggtccgt ccacacgcgg cggcggtatc
     5281 tggagaacac cctcgtgctt gagacggtgg tccgtgccgg gggggccgag gcccggatga
     5341 tcgacggcct cacgatgccg tcgggtccgg ggcgcgatcg cgagatcctg cgggcggtgg
     5401 acggcctgcg gggacggatg tcgttcgaga tcgaggtgga gccgcggttc gactgcgggc
     5461 ggctccgacc ctggatcacg cagctggggg tccactgcta tgcggcggtg gggggcgacg
     5521 actgcctgat cgtctggtcg gatgccgacc tggagccggt cggggaggat gccctgcggg
     5581 ggcgcttcga ggtgggagag ggccagcggg tccggctggc gatccggtac atccgccccc
     5641 acgagctcga cggggggcgg ggacaggatt tcgaccccac cgacctggac gatcgcctgg
     5701 agcggacgat cgagtggtgg cgggagtggg cctcgcacgg cagcatggat catccgcacg
     5761 gctccgcggc gctgcgctcg gccatcacgc tgaaggcgat gacctacgcc ccgaccgggg
     5821 cgatgatcgc cgccgccacc acctcgctgc ccgagtcgcc ccagggggcc aggacctggg
     5881 actaccggta cagctggatc cgcgattcgg ccttcgccgt gcgctccctg gcccggctgg
     5941 ggttcgagga ggaggccgac ggcttccgcc ggttcgtcga gcggagcgcg gcgggccacg
     6001 ccgaggacct tcggatcctg ttcggcgtgg gcggggagcg acacctgatg gagtccacgg
     6061 tccccgagct cgaaggctac cggggggcga aaccggtgcg gatcgggaac ggggcctcga
     6121 cccagctcca gctggacgtc tacgggcacg tggtcgagct ggcctggcgc tggcaccagc
     6181 ggggccactc cccggacgac gactactggc gcttcctcat ggacatcgtc gagacggccg
     6241 cggccagatg ggaggagcgg gaccgcggca tctgggaggt gcggggacgt cgtcagcact
     6301 tcgtgcactc caaggtgatg tgctgggcgg ccctggaccg cggcatccgt ctggccaccg
     6361 agtgcggccg gagagccccc ctccgacgct ggaggcaagt gaggcgagag atccgctcgg
     6421 ccgtcgagca gcacgggtac gaccggaagc ggggcgtgtt cacccgcagc ttcgggacca
     6481 agtcgatgga cgccgcgctg ctcctgctgc cccaggtcga cttcgtgccg gtggacgacg
     6541 agcggatgat gcgcacggtg gacgaggtcc gcaaggacct ggggcaggac ggtctgctgc
     6601 tccggtaccg cgtgtcggat gggatcgacg ctcccgaggg ggtcttcctc cccgcctcgt
     6661 tctgggcggt ggagtgcctg gccgggcagg gtcgactgga ggaggccgag gcgctgttcg
     6721 agcgggccgt ggccgccgcc aacgacctgg ggctgttctc cgaggagtac gaccccggca
     6781 cctaccacat gctgggcaac ttcccccagg ccctcaccca tatgtcgcac atcggtgcct
     6841 cggtggcgat cgcggcggcc gcgaaccaga tctcgccgcc gttgtgaccg gtaggtaggc
     6901 tgcgtcaggt ggagatccgg cccctgggct cgtccggcct gagcgtcacc ccgatcggcc
     6961 tggggctcgc cgcgctcggc cgccccgcgt acatcgacct ggggcgggag cgggacctgg
     7021 ggccggaacg ctcgcccgag gcgatgcggg cccgctgcca cgccgtgctc gatgcggcgt
     7081 tcgacgcggg gatccggtac gtggacgccg cgcgctcgta cggggatgcg gagcggttcc
     7141 tttcgacctg gctcgccggc cgcgggagca cggtggccga gctcacggtg gggtccaagt
     7201 ggggctacac gtacgtggcc gactggcgga tggacgcgga tgtgcacgag gtgaaggacc
     7261 actcgctcca gacgctccgt cgtcaggtcg gcgagagccg ggcgctcctg ggaagccggt
     7321 tggccctgta ccagatccat tcggccacgc tggagagcgg ggtcctggag gaccgcgggg
     7381 tcctggagga gctggtcagg ctgcggggcg acggtctggc gatcgggctc accaccagcg
     7441 gagcccggca gggcgagacg gtgcgccggg ccctccaggt ccgggtggcc ggggaacgac
     7501 ccttcgacgt ggtccaggcc acgtggaacc cgctcgagcc ctcggccgga ccggccctgg
     7561 cggaggccaa ggaggccggg tggggggtca tcgtgaagga ggcggtggcc aacggccgac
     7621 tcgtgcccgg cgcgccggac ctgccgaggg agctcgattc ggtggccgag cggtgcgggg
     7681 ccacggtcga tcaggtggcc atagcggccg ccctggagaa cccgtgggcc gacatggtct
     7741 tgtcgggcgc ggtgaccgaa ggacaggtcc gcagcaacgc cggggcggcc gagatcggac
     7801 tcgagcccgg tgacctggac gccctcgccg gcctggctga gccgccggag cggtactggg
     7861 ccgcgcggtc cgcgctcccc tggaggtagc cgaagcaccg ggcggcacgg gcaccgggtt
     7921 cgcgggcctg gatcacatcc gccgggccga cgggcttgca acggatggcg atccggtgcc
     7981 aaactggatg ttcctggcgt ccgagctgcg aggctcggcg atggacggcg gcgcgacgcg
     8041 ggcacgcgcc gccgtctgct ttctggcccc gtgatatcgt tcctcccccc acgggcgcgt
     8101 agctcagcgg gagagcgctg ccttcacacg gcagaggtcg caggttcgaa acctgccgcg
     8161 cccaccagcg ctcccgaagg tcggaagctc ggtcgacggg gaccgtgcgc acgtccttcc
     8221 cttaacaggc tgcggaaaaa cgccagctcg cacccgttct gaggtcgcag aggcgttcgc
     8281 cgcgtcctgg tgactcggtt atcgggagct caccactcgg tgagtggcgt gcagctctgc
     8341 ttctcggcgc tccgtcacgt cgcatgcgcg tcgagggcgg cgatccgcac cacgttgtag
     8401 accgcgcctg ccatgagaaa ctaggccctg ttcctcgctc tgccgatgaa ccggagcttc
     8461 ctgcccccgg cgatggtctt catccacccg aagggttcct cgatgcgttt gcagatccgc
     8521 tgggagacgg cgtgaccggc gtggcgggtg gtgcgggcat cgatggcgct gcgcctgtgg
     8581 tccgtctcgt tcggggcgac gtgcggggtg aagccacgct ctctgcagtc cgcgacgaag
     8641 tccttggtgt cgtaggcctt gtccccggcg agcgtccgtc tgcgtttgcg cttcggcagc
     8701 cgctcgagca tctccagggc cgcctcccgc tcggcgtacc cggtggcccg agtgagctcc
     8761 atgtccacga tgaggcccga tcggttctcc atcaggacgt gcccggcgta gcaaagcttc
     8821 gccgcggagg cgtgggactt tcgggccagc agggcctcgg gatcggtcgt tgagctgtgg
     8881 gtttggttcg agcggcgttc cccgtggaag tctcgctcgg cgttgcgtcc gccctcgggc
     8941 gtagaccccg agccatcctt cggccggaag ctcttgtggg aggcccacgc ctcgaggagc
     9001 gtcccgtcca cggagaagtg ttcggaagag gtgtagcggc ggaggtgggc ctggcggacc
     9061 acttgggcga agaaccgatc cgcgatctcg tgctccagga ggcgatccct gttcttggtg
     9121 aacgtggtgg ggtcgaaggc ctctgcctcg atggggaggt ccaggaacca cttgaaaagc
     9181 aggtcgtagt tcagccgctc gcagaaggcc cgctccgagc ggatcgagta cagggccatg
     9241 agcaccgtgg ccttcagtag ctgctccggc gggacgctgg gccgcccgat cggcgagtac
     9301 atggcctcga actcgccgtc gagcgaggcc agcaccccct cgacgacctt gcggat
//
LOCUS       DASQQQ010000101         7420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_363331, whole genome shotgun sequence.
ACCESSION   DASQQQ010000101 DASQQQ010000000
VERSION     DASQQQ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7420
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363331"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VE646_04130"
     CDS             <1..768
                     /locus_tag="VE646_04130"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZD79213.1"
                     /translation="KKAVERSLELWTSRPVVGAWSWDGDRTLYFRPREYWPAHTTVRF
                     VGHLDGVEGSPGVYGVHTLTQTFSIGRSLIAVADTGTHRVQIYLDKQLFGDWPMSSGK
                     PGDDTPNGTYLTLDKSNPEEMIGPGYDIMVPWSVRFTWSGDFLHDAYWSVGDQGFANV
                     SHGCVNLSPEHAKTYYQLAGQGDPVTITGSPRAGTWGNGWTMWFLSWPQLLRGSALHR
                     AVQTGPRGSSLVDPRALRPSRAQAPRGKPKPGNADAA"
     gene            complement(947..1405)
                     /locus_tag="VE646_04135"
     CDS             complement(947..1405)
                     /locus_tag="VE646_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZD79214.1"
                     /translation="MGGLRILVGGGDRIVLFTLPFLILGLALNDAFPSAFGGGGPAPV
                     LRIVSIVASIVGIAIWIWSVLLILMKVPRGELITTGPYAVVKHPIYTTVSFLVLPWIG
                     FLFDTWVWAGIGIVLYVGSRLFSPREEAELSERFGASWNTYLHAVRIGWL"
     gene            1635..2051
                     /locus_tag="VE646_04140"
     CDS             1635..2051
                     /locus_tag="VE646_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017199278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZD79215.1"
                     /translation="MPTKLNPYLNFRDSTREAMEFYRTVFGGKLYLSTFKDFNASQDP
                     SEDEKIMHAVLEAENGLTIMAADTPNSMEYTPGTNFNMSLSGDDEAELRGYFEKLGEG
                     GKVTMPLEKAAWGDTFGMLIDGFGVRWLVNIAGRHD"
     gene            complement(2067..2795)
                     /locus_tag="VE646_04145"
     CDS             complement(2067..2795)
                     /locus_tag="VE646_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HZD79216.1"
                     /translation="MKGLTSVERYLDSVTRQPQARGWTAPAGGAAVHWPVVTISRQTG
                     AGAHVVADELLDLLIARAPKGSPTWTVFDRNLVDEVLRHHDLPARLATFMREDRVSHV
                     ADAMEELFGLHPRASTLVKRTADTILHLAEIGNVILIGRGANLITARLDWAFHVRLVG
                     SAGKRIAYLQGRLHMDPGAAAQYLRAQDLGRQRYVKEYYGRDIDDPLQYDLVINTDGI
                     PYREAARIIADATSLLGSSPEPPS"
     gene            3083..3811
                     /locus_tag="VE646_04150"
     CDS             3083..3811
                     /locus_tag="VE646_04150"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HZD79217.1"
                     /translation="MTSTPTTMHRGPQVNRLVMIALVVLVAAAGVAAGFALGRRSGSG
                     SSSGIQGSGTAATETRDVGPFSSVDLVGSNLVSITVGDARSVVVHADDNLIHRVTTTV
                     ESGRLEIGNHGSFTTHVPMRVDVTVPHLDGVTVEGSGTVTVAGVRSDVLTVGLPGSGV
                     IKMSGTTDRLEATLDGSGDLLMGDLLAADVTALISGSGTIRLSVSRSLDASVTGSGAI
                     LYAGSPAEVSKSVTGSGTIAGSDR"
     gene            3923..4405
                     /locus_tag="VE646_04155"
     CDS             3923..4405
                     /locus_tag="VE646_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79218.1"
                     /translation="MTGRVRTSGILIVILGVWGGIVPFVGPTFGFGAGTSAWTWTVPF
                     ATLNVAAAVAAVVGGFLLLSGGASGARAGGALAVVGGMWFVLGPIFQPLWHQASVLVV
                     VTGGPSWMDVAKVIGYHDGTGLLITFLGAYALGVLSWPRRVAARSAEPVAPRRVDEAA
                     "
     gene            4534..4872
                     /locus_tag="VE646_04160"
     CDS             4534..4872
                     /locus_tag="VE646_04160"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZD79219.1"
                     /translation="MPSGLPEFRRALCEFDLPSDLLEDARLLVTELVSNSIRHAGLRP
                     TDLILVRTSWNGARLRIEVRDGGAEPGSASLCRAPGSEAGWGLYLVDRLAARWGARAG
                     RWWFELDSSG"
     gene            complement(4885..5214)
                     /locus_tag="VE646_04165"
     CDS             complement(4885..5214)
                     /locus_tag="VE646_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79220.1"
                     /translation="MGERDDRLARNEAISRTINEELERAQDAGGRDRHVRMVCECGDP
                     ACERLIAVSIAEYERVRSHPEAFLLIAEHVDPAVDRVVEETDRFVVVAKADGPPRDVA
                     EATDPRS"
     gene            complement(5292..5690)
                     /locus_tag="VE646_04170"
     CDS             complement(5292..5690)
                     /locus_tag="VE646_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79221.1"
                     /translation="MFGHVAAAISSLGPPGRAAAAGTQWSPGGPFVIAAYVATGLSLV
                     LMGVYAFAPLVRHRRRIRRARRSDRRGMSDVRDDAVERELNDLLSDQSLVREARRTVW
                     ASRRGGQDPSRHHHGPHGRRPPPDGEGHPS"
     gene            complement(5860..6612)
                     /locus_tag="VE646_04175"
     CDS             complement(5860..6612)
                     /locus_tag="VE646_04175"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZD79222.1"
                     /translation="MSAVRARWIRVLPASLTAVALSAFALIAIGRRRRSWERSERLIP
                     PPSEPRSYESPSAERAMGADRIPLEEAAAAAAECRNCPLWEGTLVYGAGNADADLMLI
                     GGTPSEEELVAFGGEADAFLDAMLHEIGVRREDVYLTTIVKHPVPGGRDPHADEIRPC
                     IHWLEEQVQLVEPKLLVPLGTVATRALLGGKVKMGHVHGERSAWKGRTVIPTFHPEWL
                     RRGGPDSQRMRDFRHDVAGIRRVLDLPQIHAA"
     gene            7199..>7420
                     /locus_tag="VE646_04180"
     CDS             7199..>7420
                     /locus_tag="VE646_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HZD79223.1"
                     /translation="MHDALFYETDERLVSALVPWLREGLDGNEGVAVVTTIPRIAMLR
                     EGLGPRAERVTFVPNDVWYTKPAATVARWR"
BASE COUNT         1127 a         2535 c         2614 g         1144 t
ORIGIN      
        1 aagaaggcgg tggagcggtc cttggagctg tggacctcga ggccggtggt cggggcctgg
       61 tcctgggacg gggaccggac cctgtatttc cggccccgcg agtactggcc ggcgcacacc
      121 acggtgcgct tcgtcggaca cctcgacggg gtggagggat cgcccggcgt ctacggggtg
      181 cacacgctga cgcagacctt ctcgatcgga cgctccttga tcgccgtggc cgatacgggc
      241 acccaccgtg tccagatcta cctggacaaa cagctgttcg gggactggcc gatgagctcc
      301 ggcaagccgg gggatgacac gccgaacggc acctacctca cactggacaa gtcgaacccg
      361 gaagagatga tcggtcccgg ctacgacatc atggtgcctt ggtcggtccg gttcacatgg
      421 tccggtgact tcctgcacga cgcctactgg tcggtgggtg accaggggtt cgccaacgtc
      481 agccacggct gcgtgaacct ctcgccggag cacgccaaga cctattacca gctggccggc
      541 cagggcgacc cggtgacgat caccgggagt ccccgagccg gcacctgggg caacggatgg
      601 accatgtggt tcctgagttg gccccaactg ctacggggaa gcgcgctgca tcgagcggtc
      661 cagaccggtc cgagggggag ttcgctcgtg gatccgcggg cgctgcggcc atcgcgggct
      721 caggctccac gggggaagcc gaagccgggg aacgcggacg ccgcgtaggc cgcggcggat
      781 ggtccactcc gatccaggtt tccctcgacg cttcctcaga ggagccccag aagctcgtac
      841 gtcgatccga tccacccgca ggcggcgcgg aacgctcccg agtcccgtcc atggcaccac
      901 cgtcccgcgt caaggccggg cgcgaagggg ccccatgatg agccgttcac agccagccga
      961 tcctcacggc gtggaggtag gtgttccagg acgcgccgaa ccgctcggag agctccgcct
     1021 cctctcgagg cgagaacagc ctggatccga cgtacaggac gatcccgatc ccggcccaca
     1081 cccaggtatc gaacaggaag ccgatccagg ggaggaccag gaacgacacg gtcgtgtaga
     1141 tggggtgctt caccacggcg tagggcccgg tggtgatcag ctcgccccgc ggcaccttca
     1201 tcaggatcag gaggaccgac cagatccaga tcgcgatccc tacgatcgac gcgacgatcg
     1261 agacgatccg caggaccggc gctggcccgc cgcccccgaa ggccgacggg aacgcgtcgt
     1321 tcagggccag accgaggatc aggaagggga gcgtgaacag cacgatcctg tcaccgccgc
     1381 cgaccaagat cctcaggccc cccaccgccg ttcgagccgg cccgcaccat cccgcgccat
     1441 cggcccgggc tggcccctcg ctccgatcgt gcccaccgcc ctcacctcgc gtcgtaccgg
     1501 cgcccctcag gggcgccgaa ccggccctgg tcggactcaa gcctcgccgg tcgagcggac
     1561 accggttagg agagatggtc ctctccgggg cagggccagt agcatcgagg ggtccacgag
     1621 gaggggagga gcggatgccg accaagctga acccgtacct gaacttccgg gactccaccc
     1681 gcgaggcgat ggagttctac cggacggtct tcgggggcaa gctctacctc agcacgttca
     1741 aggacttcaa cgcctcccag gacccgagcg aggacgagaa gatcatgcac gccgtcctcg
     1801 aggccgagaa cggcctcacg atcatggctg cagacacccc gaacagcatg gagtacacgc
     1861 cgggcacgaa cttcaacatg tcgttgagcg gtgacgatga ggccgagttg cgcgggtact
     1921 tcgagaaact cggagagggc gggaaggtga cgatgccgct ggagaaggcg gcttggggcg
     1981 acaccttcgg gatgctcatc gacggcttcg gggtccggtg gctcgtgaac atcgccggga
     2041 gacacgacta gggatgcatg tcgcgctcag ctgggaggtt cgggggaaga tccgaggagg
     2101 gaggtcgcgt cggcgatgat ccgggcggcc tctcggtacg ggatgccgtc cgtgttgatc
     2161 acgaggtcgt actggagcgg gtcgtcgatg tcccgtccgt agtactcctt cacgtaccgc
     2221 tggcgaccga ggtcctgcgc acgtaggtac tgggcggccg ctcctgggtc catatgcaga
     2281 cgtccctgca ggtaggcgat ccgcttgccc gccgaaccca ccagccgcac gtggaacgcc
     2341 cagtcgagcc tcgccgtgat gaggttggca ccacggccga tcaggatcac gttcccgatc
     2401 tccgccaggt gcaggatggt gtccgccgtc ctcttgacca gcgtcgacgc ccgtgggtgg
     2461 agcccgaaga gctcctccat ggcatccgcc acgtgagaga cgcgatcctc gcgcatgaac
     2521 gtcgcgagcc gggccggcag gtcatgatgc ctgagcacct cgtccaccag gttccggtcg
     2581 aagacggtcc aggtgggcga gcccttgggc gctcgagcga tgaggagatc gaggagctca
     2641 tcggcgacca catgggctcc cgctcccgtc tggcgggaga tcgtcaccac gggccagtgc
     2701 accgctgctc cacccgccgg agcggtccat ccccgtgctt ggggctgtcg cgtgaccgag
     2761 tccaggtagc gctcgacgct ggtgagcccc ttcatgaacg gctcacccca tcctccacgg
     2821 ggcgctcggt ggctcctcca gcctcgatcg agggctccac gatctccctc tcctcgcacc
     2881 catggtaccg aggggcaggc atcgagccct acggcggccg gttacccgcg accacggacc
     2941 gggatcgagc ggcatacgct ccgtccctcg gcgccgggga ccatcgtccc tcccggaccc
     3001 atcggggacg gacacagttg tggtgcgcca ccggaacggt gacggctctc gatgtccacc
     3061 gaaccgccgc gaggagaggg ccatgacgag caccccgacg accatgcacc gtggtccaca
     3121 ggtcaaccgg ctggtgatga tcgccttggt cgtgctcgtc gcggccgccg gtgtggcggc
     3181 cgggttcgcg ctcggtcggc ggtccgggtc cggatcgtcg agcggcatcc agggatccgg
     3241 cacggcggcg accgagacgc gggacgtcgg cccgttcagc tccgtcgacc tcgtcgggag
     3301 caacctggtg tcgatcaccg tcggcgacgc tcgcagcgtc gtggtgcatg ccgacgacaa
     3361 cctgatccac cgcgttacca ccaccgtcga gtccggccgg ctcgagatcg gcaaccacgg
     3421 cagcttcacc acacacgtac cgatgagggt cgacgtgacc gtcccccacc tcgacggggt
     3481 cacggtggag ggctcgggaa cggtgacggt ggcgggcgtg cggtcggatg tgctcacggt
     3541 cgggttgccg gggagcggcg tgatcaagat gtcgggcacc accgaccggc tggaggcgac
     3601 gctggatggc tcgggtgacc tgctgatggg cgacctgctc gccgcagacg tcacggccct
     3661 gatctcgggc tcgggaacga tccggctgtc cgtgtctcgg agcctcgatg catcggtcac
     3721 cgggagcggg gcgatcctgt atgcgggcag cccggcggag gtcagcaaga gcgtgaccgg
     3781 ctccggcacg atcgccggca gcgatcgctg aaaaggccgt tacgtccgct cgacgccgtt
     3841 ccacccctgc ccgctcgtcc gtttagcccg tgcacgctgc ggggaagggg caatcacgag
     3901 tccgccacgg gaggtggatg tcatgaccgg acgggtgaga acgagcggga tcctgatcgt
     3961 catcctgggt gtgtggggtg gcatcgtgcc cttcgtgggc ccgacgttcg ggttcggggc
     4021 ggggaccagc gcctggacct ggaccgttcc cttcgcgacc ctgaacgtcg cggcggcggt
     4081 ggccgcggtc gtgggagggt tcctgctgct atccggcggg gcgtccggtg cccgggccgg
     4141 cggcgcgctc gccgtggtcg gagggatgtg gttcgtgctc gggcccatct tccagcccct
     4201 gtggcaccag gcgagcgtct tggtcgtggt caccggagga cccagctgga tggacgtggc
     4261 caaggtgatc gggtaccacg acggcacggg gctgctgatc acgttcctcg gcgcgtacgc
     4321 cctcggcgtg ctgtcctggc cgaggcgcgt ggccgcccgg tccgcggagc ccgtggcgcc
     4381 gcgccgggtc gacgaggcgg cctgaccata ctgggggctt gttcccccga ccgtgtatac
     4441 cggcgagccg gtgggtaggt ggcgaccagt cggatgggag ccaggaccgc ccctcgtgac
     4501 ggcgggcggg tatcggaatt gagggtcccc gccgtgccgt ctggcttgcc cgagttccgc
     4561 cgcgccctct gcgagttcga tctcccctcg gatctgctgg aggatgcgcg gctcctggtc
     4621 accgagctcg tgtccaacag tatccgccac gccgggcttc ggcccaccga cctcatcctg
     4681 gtccgcacgt cgtggaacgg ggcccggctc cgcatcgagg tccgcgacgg cggggcggaa
     4741 ccgggatctg cgtcgttgtg tcgcgcaccc ggctcggagg ccggttgggg tctgtacctg
     4801 gtcgaccgtt tggccgccag gtggggggcc agggccgggc gctggtggtt cgagctcgac
     4861 agctccgggt gatggtgacg gggatcagga ccgagggtcg gtggcctcgg ctacatctcg
     4921 aggcggaccg tcggctttgg ccaccacgac gaaccgatcc gtctcctcca ccacacggtc
     4981 gacggcagga tcgacgtgct cggcgatcag caagaatgcc tctgggtgcg accggacgcg
     5041 ttcgtattcc gcgatggaga cggcgatcag ccgctcacag gcgggatccc cgcactcaca
     5101 gaccatccgg acgtggcgat cgcgcccccc cgcatcctgg gcccgctcga gctcctcgtt
     5161 gatcgtccga gagatggcct cgttgcgggc caaccgatcg tcccgttccc ccatcgcccc
     5221 cctcccggac gctagcacga gggatgcgac ctggggaagg cggccggtcc gtccggtgat
     5281 gagtggtacc gctacgaggg gtggccttcg ccatccggcg gcggacggcg tccgtggggt
     5341 ccgtggtggt ggcgtgaggg atcctgccct cctcgacggg aggcccagac cgtcctgcgg
     5401 gcttctcgga cgagggactg gtccgacagc aggtcgttca gttcccgctc gaccgcgtcg
     5461 tcgcggacgt ctgacatccc gcgccggtcg gagcgacgtg ccctccggat ccgtcgtcga
     5521 tggcgcacca acggcgcgaa cgcgtagacc cccatcagca ccaggctcaa ccccgtggcg
     5581 acgtaggcgg cgatcacgaa cgggccccca gggctccact gcgtccccgc ggccgccgcc
     5641 cggccgggcg ggccgaggga cgagatcgcg gctgccacgt ggccgaacat cggctcctcc
     5701 agcggtcctc gggtcccgac ccagggaagt atggccccaa gatcggtcgt ctgctcaccc
     5761 ctatcgagat ggtgccccgc gctccgaaga ccgaaacgac cggaggcgat ccgaggggca
     5821 tgccgaggcg acgagccagc gctcgagcct caggcgggct caggcagcgt ggatctgcgg
     5881 cagatccagc acccgtcgga tccctgcgac gtcgtgccgg aagtcccgca tccgctgcga
     5941 gtcgggccca ccgcggcgga gccactcggg gtggaaggtc gggatgacgg tgcggccctt
     6001 ccacgcggac cgctccccgt gcacgtgacc catcttcacc ttcccgccca gcagcgcgcg
     6061 ggtcgcgacc gtcccgagcg gtacgagcag cttcggctcc accagctgga cctgctcctc
     6121 cagccagtgg atgcagggcc ggatctcatc ggcgtgcgga tcccgccccc ccgggaccgg
     6181 gtgcttcacg atggtggtga ggtagacgtc ctcgcgccgg acgccgatct cgtggagcat
     6241 cgcgtccagg aaggcatccg cttcgccccc gaaggcgacg agctcttcct cggacggggt
     6301 cccgccgatc agcatcaggt cggcatccgc gtttccggcg ccgtacacga gggtgccctc
     6361 ccagagcggg cagttgcggc actcggccgc tgcggccgcc gcctcctcga gggggatccg
     6421 atcggccccc atcgctcttt ccgcagacgg ggactcgtag gagcggggtt cgctcggcgg
     6481 tgggatcagc cgctcggagc gttcccaact tcggcgccgc cgaccgatcg cgatcagcgc
     6541 gaaggcgctc agggcgacgg ccgtcagcga tgcgggcagg actcggatcc atctggcgcg
     6601 cacggcgctc acctctccca tccgaggatc gaggagatcg tcacggtccc atcggcgtcg
     6661 ggcgggtctt ccttgacctc catgtgatcg agacatcctc ctcgtcggcg gatctgtgca
     6721 ccacgtaccc atgtcaccgg cgcgcgatgc ccgcaaacgc gatcggaatg tggtgtcgcg
     6781 tgccccggcg ctgtgcggca ggtggagcgt ccgatccatg gcgagacccc cgcgtccggg
     6841 ggccccactg aagcgatgcc ctgcgcggcc cgcgtccggg ctccggaagg tcggagcggc
     6901 cgcgcttcac gccgagccct tggccgttgg gctcgggtcg ggagcggacc ccgctcgcgc
     6961 tcgtcccctc gggcccgaac cgtatcacaa gcgtgtgatc cggtcgagcc cgcggtccga
     7021 tagggcaacg agccggccgg gtaggacctg ttcggtggat ggcccacggt ggcttgctct
     7081 tccgcgcagg ccggcgctac cctcgtccgg gcaggcgtct gcgatggggt gcgccggcca
     7141 cgggcaagat gacgctcctc ggcgtggagg tggttccctg acgcgcgcgg ctggactggt
     7201 gcacgatgcc ctgttctacg agaccgatga gcgcttggtc tcggcgctcg tgccgtggct
     7261 gcgggagggg ctcgacggga acgagggtgt ggccgtggtc accaccatcc cccgcatcgc
     7321 gatgctccga gagggcctcg gaccccgggc ggagcgggtg accttcgtgc cgaacgatgt
     7381 ctggtacacg aagccggccg caaccgtggc caggtggcgg
//
LOCUS       DASQQQ010000102         4367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_224630, whole genome shotgun sequence.
ACCESSION   DASQQQ010000102 DASQQQ010000000
VERSION     DASQQQ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224630"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="VE646_04185"
     CDS             <1..1090
                     /locus_tag="VE646_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79224.1"
                     /translation="FAAIGALAAQLFDSRRRAATWAGIALALSLMLRVAADGTRTLGW
                     IRWTTPLGWIENVRPFGGTNLVALIPLSVSIVAAVTGALVLRARRDQGAGALGLEEAA
                     ARRTRLLTGPEGLALLSGRTAAVTWTLAVGAFAFVFGLLAKGVSEFIEEGEGFEEILR
                     RFGGSGILSAAGFLGLIYSAFIVIVLVVYAGTQISAMREEEATGRLDNVLVHPVGRAR
                     WLATRGMVAVVGAVVVAVVASLAMWAGAAAAGSSVSLWGQVRAALNCLPVVALFLGIG
                     LLAFGFVPRLTAAITFGLVTVAYLIQLIGAIVDAPDWVLDLSPFHHVAAVPIVPANVG
                     AALAMLAAALIAIVAGTAAWLRRDIALA"
     gene            1281..1496
                     /locus_tag="VE646_04190"
     CDS             1281..1496
                     /locus_tag="VE646_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018518417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HZD79225.1"
                     /translation="MCRSIKRLRAADGELATDEQIREAALQFVRKVSGYRAPSKRNTQ
                     AFEAAVDEVADASRRLLEAVAPPVPRR"
     gene            1732..3123
                     /locus_tag="VE646_04195"
     CDS             1732..3123
                     /locus_tag="VE646_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZD79226.1"
                     /translation="MVPLDLSPEQMREMGEAALAYVVDFLAGRDAAAASNFDEAEAVA
                     RLHRASPPQEGGDFGPLVELVDTIASNSADNAGPGFLAYIPGGGLFASSLADLLSTTI
                     DRFVNLWGEAPVAAQIENNVVRWLCGLFDLPPESRGVLTSGGSMANFSAIVTARAAHL
                     PEDFLHGALYVSEHVHASVQKAAMLAGFPVRNVRRVPVTPDLRMDVQALRKMVGEDRG
                     TGFAPFGVVASAGTTNTGTIDPLSAIAEVCEVEGLWLHVDAAYGGFFQLTERGHERFA
                     GIERADSITLDPHKGMFLPYGTGALIVRHGTKLREAHFVGAAYLQDLAADADIPNFTE
                     YSAELSRDFRGLRVWFPLKLHGVSAFRRALDEKLDLSELLYEELKADPRLELPWSPDL
                     TVVPFRLRDGDDAANRRLLETVNASKRVFLSSTVIDGRFTIRACILSHRTHRDRIEEC
                     IAIVRDAVARIAG"
     gene            3238..3471
                     /locus_tag="VE646_04200"
     CDS             3238..3471
                     /locus_tag="VE646_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HZD79227.1"
                     /translation="MRKLDVLTILLVVVGALNWGLVAIAEFDLVAAVFGMDFGQTSAI
                     TRVVYGLVGLSGIYVGAQASSMRRRWSARLIAA"
     gene            3563..>4367
                     /locus_tag="VE646_04205"
     CDS             3563..>4367
                     /locus_tag="VE646_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HZD79228.1"
                     /translation="MPELPEVQALAERLHAALAPAPFAGADLLSFSSLKTVSPRASEL
                     VGRELSGVGRRGKFLLLDLEGPRLLVHLSQGGRVDVEDPPKSTRPRGAVVRLRFEGRA
                     SVLVKEFGRERKAGWWIVAPGDEGPLGRLGPEPDEPAFERLVLEGDDARRVHTILRDQ
                     HTVAGIGRGYADDILHRARLSPFAALGSLSAEQRRALLEAVHAILDEALQVERRRTGG
                     LPTKIGDHFTVHGRHGMPCPACGDDLRRISYGSHEVTYCPACQTGGKVLA"
BASE COUNT          542 a         1519 c         1615 g          691 t
ORIGIN      
        1 gttcgcggcg atcggggcgc tcgccgcgca gctgttcgac tcgcggcgtc gggccgcaac
       61 ctgggccggg atcgcgctgg ccctctcgct gatgctccgg gtggccgccg acgggacccg
      121 cacgctcggg tggatccggt ggaccacgcc gctcggctgg atcgagaacg tacgcccgtt
      181 cggggggacg aacctcgtcg cactgatccc gctctcggtg tcgatcgtgg cggcggtgac
      241 cggcgcgctc gtcctgcggg cccggcggga ccagggagcc ggggcgttgg ggctcgagga
      301 ggcggccgcc cgcaggacca ggttgctcac cggccccgaa ggcctggcgc tgctgtcggg
      361 ccggaccgct gccgtcactt ggaccctggc cgtgggggcg ttcgcgttcg tgttcgggct
      421 gctggccaag ggggtgagcg agttcatcga ggagggcgag gggttcgagg agatcctgcg
      481 ccggttcggc ggctcgggca tcctgtccgc ggcggggttc ctggggctga tctactcggc
      541 cttcatcgtg atcgtgctgg tcgtctacgc gggcacgcag atctcggcga tgcgcgagga
      601 ggaggccacc ggacggctcg acaacgtcct ggtccatccg gtggggcggg cccggtggct
      661 cgccacgcgg gggatggtgg cggtggtcgg cgcggtggtg gtcgcggtcg tggcgagcct
      721 ggcgatgtgg gccggggccg ctgcggccgg ctcctccgta tcgctgtggg ggcaggtccg
      781 ggccgcgctg aactgcctgc cggtggtggc gctgttcctg ggcatcggcc tgctggcctt
      841 cggcttcgtg cctcgcctca cggcggcgat cacgttcggc ctggtgacgg tggcctacct
      901 gatccagctg atcggggcca tcgtggacgc gccggactgg gtgctggacc tgtccccgtt
      961 ccaccacgtg gcggccgtgc ccatcgtgcc ggcgaacgtc ggggccgcgc tggcgatgct
     1021 cgcggccgcg ctcatcgcca tcgtcgccgg caccgcggcg tggctccgtc gcgacatcgc
     1081 cctggcctga gggaagttgc acagcgcgat gggccgttcg cgtccctgga agaggtcacg
     1141 ctacgttggt gcttctcgag gtctcgatgc ggcggatatg cggccccgat ttcgaaccgc
     1201 gggtgagcgg tccggaacgg ctgccgccgc agtcgggcga gaccctccgg gccgttgggc
     1261 aggatggcta tcatcggacc atgtgccgga gcatcaagcg gttgcgggcc gcggacggtg
     1321 agctggccac cgacgagcag atccgggagg cggcgctcca gttcgtgcgg aaggtgagcg
     1381 ggtatcgcgc gccctccaag cggaacaccc aggcgttcga ggcggcggtg gacgaggtgg
     1441 ccgacgcctc gcgccggctc ctcgaggcgg tggccccgcc cgtccctcgg cggtgaaggt
     1501 cgtccccctc accgagcggc ccgatctgga cgagcgcgtg agcgagcacc tcctcgatgg
     1561 cgtggacggc gtgcttccct cgcggtggag cagcggaacg atgggctgcc gcccaccacg
     1621 ctctgcgcgc tcgagggtgt ggtcgcgccc gagaagcagg acggggccac ggcctcggca
     1681 ccgtctctcg tgaacgtcga ccgtccgtga cgcgtgggta ggatgggcgt cgtggtgccg
     1741 ctggatctct caccggagca gatgcgcgag atgggggagg ccgcgctcgc ctacgtcgtg
     1801 gacttcctgg ccggtcgcga cgccgcggcg gcgtcgaact tcgacgaggc cgaggccgtg
     1861 gcccgtctcc accgggcatc ccctccgcag gagggcgggg acttcgggcc gctcgtggag
     1921 ctggtcgaca cgatcgcctc gaactcggcc gacaatgccg ggcccgggtt cctggcctac
     1981 atccccggcg gcgggctgtt cgcctcgtcc ctggccgacc tgctgtcgac cacgatcgat
     2041 cggttcgtga acctgtgggg ggaagccccc gtggccgcgc agatcgagaa caacgtggtg
     2101 cggtggctgt gcgggctgtt cgacctgccg ccggagtccc gcggcgtgct cacctccgga
     2161 ggctcgatgg ccaacttctc cgccatcgtg accgcccgcg ccgcccacct gcccgaggac
     2221 ttcctgcacg gcgccctgta cgtgagcgag cacgtgcacg cctcggtcca gaaggcggcg
     2281 atgctggcgg ggttccccgt ccgcaacgtc cggcgcgttc ccgtcacgcc cgacctgcgg
     2341 atggacgtcc aagctctccg gaagatggtc ggggaggacc ggggaaccgg gttcgccccg
     2401 ttcggggtgg tggcctcggc cggcaccacc aacaccggca cgatcgaccc gctctcggcc
     2461 atcgcggagg tgtgcgaggt cgaggggctc tggctgcacg tcgacgccgc gtacgggggg
     2521 ttcttccaac tcaccgagcg ggggcacgag cggttcgccg ggatcgagcg ggcggactcg
     2581 atcacgctgg atccgcacaa gggcatgttc ctcccgtacg ggaccggcgc cctgatcgtc
     2641 cggcacggca ccaagctccg tgaggcgcac ttcgtggggg ccgcctacct ccaggacctg
     2701 gccgccgacg ctgacatccc gaacttcacc gagtactcgg ccgagctctc cagggacttc
     2761 cgtgggcttc gggtctggtt cccgctcaag ctccacgggg tctcggcgtt ccgtcgggcg
     2821 ctggacgaga agctcgacct gtcggagctg ctctacgagg agctgaaggc cgacccccgc
     2881 ctggagctgc cctggtcccc ggacctcacg gtggttccct tccgcctccg cgacggcgac
     2941 gacgccgcca accgccggct gctcgagacg gtcaacgcct ccaagcgcgt cttcctgtcc
     3001 agcaccgtga tcgacgggcg cttcaccatc cgggcctgca tcctctcgca ccgcacccac
     3061 cgcgatcgca tcgaggagtg catcgcgatc gtgcgggacg cggtggcccg gatcgccggg
     3121 taacggagcg gccgtccccg tcgaacctat gcactgggtg cgcaatcgtg gacactcccg
     3181 cataccttaa cgcacactgg tcccacacgt ccgcataggt tcgaaggagg ggtagcggtg
     3241 aggaagctcg acgtcttgac catcctgttg gtcgtggtgg gcgccctgaa ctggggcctg
     3301 gtggccatcg cggagttcga cctggtcgcc gccgtgttcg gcatggactt cggtcagacc
     3361 agcgcgatca cccgcgtggt ctacgggctg gtcggcctgt ccggcatcta cgtgggggcc
     3421 caggcctcct cgatgcggcg ccggtggagc gcccggctga tcgccgcctg acccacccgt
     3481 accaccaccc gagcaccgca gacgaggagc cgccggcccg cgccggcggc tccacgtcgt
     3541 gcacgctggt tacagtgacg gcatgcccga gctccccgag gtccaagcct tggccgagcg
     3601 gctccacgcc gcgctggctc cagcgccctt cgccggcgcc gacctgctga gcttctcctc
     3661 cctgaagacg gtgtcgcccc gggcctcgga gctggtgggg cgagagctgt cgggggtggg
     3721 tcgccggggc aagttcctgc tcctcgacct ggagggtccc cggctcctgg tgcatctatc
     3781 ccagggcggc cgggtggatg tggaggatcc tcccaagtcg acccggcccc ggggggccgt
     3841 ggtgcggctc cggttcgagg ggcgagcatc cgtcctggtg aaggagtttg ggcgggagcg
     3901 caaggccggc tggtggatcg tcgcgccggg agacgagggg ccgctgggcc gcctcggccc
     3961 cgagccggac gagcccgcct tcgagcggct ggtgctcgag ggcgacgatg cgcggcgcgt
     4021 gcacacgatc ctccgggacc agcacaccgt ggccgggatc ggccgcggct acgcggacga
     4081 catcctgcac cgggcccgac tctcgccgtt cgcggccctg gggtcgctct cggccgagca
     4141 acgccgggcg ctcctggagg ccgtgcacgc catcctggac gaggctctcc aggtggagcg
     4201 gcgacgcacc ggtggcctcc ccacgaagat cggcgaccat ttcacggtgc acgggcgcca
     4261 cggcatgccc tgcccggcgt gcggcgacga ccttcgccgg atctcctacg gctcccacga
     4321 ggtcacctac tgtcccgcct gccagaccgg aggcaaggtg ctggccg
//
LOCUS       DASQQQ010000103        24978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_307530, whole genome shotgun sequence.
ACCESSION   DASQQQ010000103 DASQQQ010000000
VERSION     DASQQQ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 24978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24978
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307530"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VE646_04210"
     CDS             complement(<1..193)
                     /locus_tag="VE646_04210"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HZD79229.1"
                     /translation="MSVPPPPPPPPPSRQPTRSLRRSRSDRVIAGVCGGIGWYLGVDP
                     VLIRIAFVVLTVAGGSGVLL"
     gene            complement(245..2836)
                     /gene="clpB"
                     /locus_tag="VE646_04215"
     CDS             complement(245..2836)
                     /gene="clpB"
                     /locus_tag="VE646_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HZD79230.1"
                     /translation="MDLQKLTMKSQAALEAAREQALARNHQAIEPEHVLFALLSDPEG
                     VVYPLLHRLGTSPRALRDRIEALLERIPKVYAQGAAEVRVSSATASLLEEALREAQAL
                     TDEYVSTEHLFLAMLRGDSNVARVLRESGITRDAALAALAEVRGRQRVTDPNPEEKYQ
                     ALERYGRDLTELARRGKLDPVIGRDDEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVE
                     GLAQRIVDGDVPESLKDKRVVALDIGAMVAGSKYRGEFEERFKAVLREIAESEGQVIT
                     FIDELHTIVGAGGAEGAVDAGNMLKPMLARGELRAIGATTLDEYRQHIEKDPALERRF
                     QPVYVGEPSVADTIAILRGLKERYEVHHGVRIQDSALVAAAVLSNRYVTGRFLPDKAI
                     DLIDESASRLRIEIDSMPVEIDEVERRIKQLEIERAALKKETDPVSAERRDRLEAELA
                     DLREQAEGMKAHWRREKEQIDRIRSLKSEIEQARMEAERAERDGELERAAELRYGRLV
                     ELTKRLEEANTELAELQRDRKMLKEEVDEEDVAEVVSKWTGIPVARLMEGEMDKLVHL
                     EGALHRRIVDQDEAVEAVANAIRRSRAGLSDPDRPIGSFIFLGPTGVGKTELARALAE
                     FLFDDERAMVRIDMSEYQERHTVSRLIGAPPGYVGYEEGGQLTEAVRRRPYSVVLLDE
                     IEKAHTDAFNVLLQLLDDGRLTDGQGRTVDFRNAVVIMTSNLGSYVFADPMASIEERK
                     EKVMAEVRATFRPEFVNRVDEIVVFHQLGREEIAQIVDIQLGQLQRRLSERNLTFEPT
                     DAARDLLADKGFEPAYGARPLKRLIQREIQDALAMKLLSGELREGDRVEVDAGPDGLV
                     FRAHQPA"
     gene            complement(2923..3372)
                     /locus_tag="VE646_04220"
     CDS             complement(2923..3372)
                     /locus_tag="VE646_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016695959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZD79231.1"
                     /translation="MRDRGGDDGMKRQGQADRREDRAVYIISVAAELAGVHPQTLRIY
                     ERKGLVRPKRTSGNTRRYSERDIRRLQLIQELTQEQGVNLAGAKMIIEMQAELEDLRR
                     RTAELESELRRVRRGRPVPLGPSSATVDIVPVRTVVSFPWAGHRVVT"
     gene            complement(3365..4507)
                     /gene="dnaJ"
                     /locus_tag="VE646_04225"
     CDS             complement(3365..4507)
                     /gene="dnaJ"
                     /locus_tag="VE646_04225"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZD79232.1"
                     /translation="MNGDVRREWFEKDYYQVLGVAKNAPQDEIKKAYRKLAQKYHPDA
                     NPGNETAEERFKEISAAYDVVGDADKRKQYDQVREMASSGYGPGGFPGAGPGGAGGRV
                     RFEDVGFGGMGDIGDLFSVFTGGGRRTGTGRPRGADLETEVRVSFEDAMRGTTVPVRI
                     QGPAPCPRCGGSGAEPGSSPVTCPQCGGAGAVSVNQGFFQMTQTCPRCHGSGHIVEHP
                     CTQCRGSGSIRSTRRFNVKVPAGVKDGARIRLAGRGEPGPAGGKPGDLFVVVHVASHA
                     LFGRKGNDLTITVPVTYAEAALGANVEVPTLNGPVTLKVPAGTQGGRTFRIRGRGAPK
                     KGGHGDLLARVEIEVPRKLSREEKQLLRQLKEMEKDRPRKRLGVDA"
     gene            complement(4517..5137)
                     /locus_tag="VE646_04230"
     CDS             complement(4517..5137)
                     /locus_tag="VE646_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZD79233.1"
                     /translation="MDRPRVRVTDKRRVRDEGGSAPAEAGAAPPADPAEEEGLAPTPE
                     APTSGSTTEASGTVDLEAARQEAAAYRDHLVRLQAEFENYRKRVLKEQTRAVEMAAEP
                     LIRRLLEVIDEFELALMAAEQKPDFDKFLHGVELVYAKLMDTLKAEGLERIEAEGKPF
                     DPELHEALMGSAGGEGDLVVGDVLRQGYTLRDRVIRPSGVRVTHEA"
     gene            complement(5167..5619)
                     /locus_tag="VE646_04235"
     CDS             complement(5167..5619)
                     /locus_tag="VE646_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZD79234.1"
                     /translation="MRETLVRWDPFRDLVSIQDELNRLFGRTFAGAEATRAVGAGTWM
                     PALDVYETEDRFVAKLELPGIDPGAVDVSVEDSTLIISGKREFESEVNEEDYHRIERR
                     YGAFTRSIGLPQTADTEKVEASFDKGVLTVEVPKIEKAKPKKITVKAK"
     gene            complement(5616..7478)
                     /gene="dnaK"
                     /locus_tag="VE646_04240"
     CDS             complement(5616..7478)
                     /gene="dnaK"
                     /locus_tag="VE646_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZD79235.1"
                     /translation="MPRAVGIDLGTTNSVVSVLEAGEPVVIPNAEGSRTTPSVVGFSK
                     NGEILVGEVAKRQAITNPDRTIRSVKRHMGERDWSVEIDGKKWTPQEISAQILTKLKR
                     DADAYLGDTATQAVITVPAYFDDAQRQATKEAGQIAGLEVLRIINEPTAAALAYGLDK
                     QHDQTILVFDLGGGTFDVSLLEIGEGVFEVKATHGDTHLGGDDWDQRVIDWMVKEFKN
                     AHGVDLGADRMALQRLKEAAEKAKIELSQVAETTINLPFITATAEGPLHLEMKLTRSE
                     FERMTEDLVERCRSPFDQAVKDWGKDVSAIDHVILVGGATRMPMIQELVKKLTGGKEP
                     HKGVNPDEVVSVGAAIQAGVLKGDVKDILLLDVTPLSLGVETKGGIFTKLIERNTTIP
                     TRKSEIFTTADDGQTTVEIHVLQGEAETVYSPGVRSLGKFHLMGIPPAPRGMPQIEVA
                     FDIDANGIVNVSAKDLATGKEQQMTITGGTALSKDDIDHMVKDAEKFAAEDHRRREAA
                     EARNSADQLTYQIDKAIEEWGDKVPQGDRDQLTRANDELKEALRGEDVDTIRSATDAA
                     MQAFQRIGQAMYQTTQAQAEPGQQAAAAGGGEAASEGGDEDVVEGEIVDEGGAK"
     gene            7685..9685
                     /locus_tag="VE646_04245"
     CDS             7685..9685
                     /locus_tag="VE646_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HZD79236.1"
                     /translation="MAARIVALLALLMVAFGLFARRVALLARLARLGRPVERARDVPT
                     RVEREVTVVFGQRKLLQRLVPGLMHAFIFWGFLVLLTTIAEAIGQAVSPTFALPVLGH
                     AAWLGFVQDLFAALVVVGIVMALLIRKVQRPERFVGSHLREADLILAWIFGVVLTLFG
                     LNATRIAQGLIESPAAWMPISNALAALFRPLAPSTLQTLAWAFLWSHLVLVFGFLVFV
                     PYSKHLHIFVSFPNVFLSNTKPRGYLTPLRIDLEALETEDVGLGAASIEDLSWKQMLD
                     LYACTECGRCQGVCPAWNTGKPLSPKLLIMNLRDHLFEQGPKILQARADGTELAAVAL
                     NPDVVDDEVLWSCTTCGACMQECPVDIEHIDHIVDMRRSLVMAESRFPQEAGALLRNL
                     EGAGNPWGQPQDERAAWASDMDVPVVADGGPPFEYLYWVGCAGSFDDRAKAISRAVAT
                     LLRRAGLSFAILGAQERCTGDPARRMGNEYLFQTLAEQNVEILNRAGVTKIVANCPHC
                     YNTLLNEYPDYGGRFEVIHHTQLFRRLVAEGRLRPSAGVRSLVTYHDPCYLGRHNEIY
                     AAPRQLLDGVPGVATVEMPRHGPRGLCCGAGGARMWMEERIGKRINEERMDEAASTGA
                     DTVGVACPYCLVMLDDGARSRGEGTEVADVAQILVRAVEPGD"
     gene            9927..10892
                     /locus_tag="VE646_04250"
     CDS             9927..10892
                     /locus_tag="VE646_04250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZD79237.1"
                     /translation="MDTIRDRADRAAECRRLAEQLQLDAVGIVRSGPDGRELSWWAAP
                     GGPPRPLDVDEVLTGHAAGWVVCPRSDEVVFGRLTPESSVRSVQVLRAMLSDLSGEPP
                     RTAAPGAASPDDPVARERARWAYAIHDGLTQVVTAAVLDLEWRARKLELEPGEAITAL
                     SEAASELHSALEDVRGVLAILTPPEPLEEESLDHLVEELFGRWQLPSSWSIEGDLDSI
                     PRPILEAASSIIRESVANAAKHSSTQEVNVRIQASGQEMEVSVEDRGRGFRPTETGLH
                     AGHLGLEMMRRRVAELHGSLDIRSEPGHGTRVVARLPVGEQGDEP"
     gene            10898..11596
                     /locus_tag="VE646_04255"
     CDS             10898..11596
                     /locus_tag="VE646_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD79238.1"
                     /translation="MIVDDHALVRRGMAHVVRECFADADVVEAADAAEAMGVMERTPA
                     DIALVDVRLPVHDGLELLHDMKSRWREVPVIMLTTFDHAQYVRRALAEGAAGYLLKDA
                     TPEDLEQAIRVALSGGGNVLSPRVIQNLFEAMETGEGEEEAPHRRPAANLTQRETDIL
                     ELLAEGKSNRDISRSLFLSEKTVKAHLAAIFRKLGVSNRTQAAMAAVSMGIGSAHFPV
                     DRAEAVGSGAGDRS"
     gene            11642..13129
                     /locus_tag="VE646_04260"
     CDS             11642..13129
                     /locus_tag="VE646_04260"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZD79239.1"
                     /translation="MAESEGTRSNGDRGTTTAEATGAVGPGGFGHPGPVEAYEEDWYR
                     SLKALAERRRDLLGEERDAPEGPTGPFSEEPVAHSEATEGPVQDPDDEIQTVARQEAE
                     RAGDELAQRDRAVRALGHCTQGLPGPAMELALADPDPDVRATALQWLPPMPERGLADM
                     VSRSLGDPDERVRRAAMRRFAECAGADRDAAWNALRTCPAKELGDMVAALQQTQTASI
                     ELAFERLSSSEEGERVLAIEVVGWGRSPGCVEAAVHALGDPSAPVRRAAVASLGRLRD
                     PSAAVALNKALVDPDPDVRVGAVNALGVIDDESVLGSLVTALKDPEPRVREMASEVLT
                     EWSSPAVARRLAEVLAVPSQRDSAAGLLMRIGPASLELLIDVLRQGNQVLTPIVGGLL
                     QRIVGFEDLLGRFSSPDPDRRLRAVEAVAAIGGSPAVDALIGMLSDRDERIRIRSAQL
                     LGALGDRRAEQPIRQAALEDPVPEVVQAAAETLARLGPEDGGRAA"
     gene            complement(13132..14154)
                     /gene="trpD"
                     /locus_tag="VE646_04265"
     CDS             complement(13132..14154)
                     /gene="trpD"
                     /locus_tag="VE646_04265"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018761367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HZD79240.1"
                     /translation="MPDPDLWPSILSRLIGGGSLSAAESSQALGSIMREEATPVQVAG
                     FLMALRTKGETVDEVEGLARAMLEFARPVTFRGSLVDTCGTGGDRASTFNISTVSALV
                     VAGAGVRVAKHGNRAASSRCGSADLLEALGVKIDHEPEGVERCLAEAGIGFMFAPVFH
                     PATAYATGIRRELRVPTIFNFLGPLTNPARPAAQVVGVSDARMLPLVAEVLARRRVRA
                     LVFRGLDGLDELTTTGPSTVFRVRDGSASEGTLDPFELGLPPATPEDLRGGEVAENLG
                     IARRVLDGQAGPARDVVLLNAGAALEVAGRADSLAQGMELAATSIDSGAAGQILERWV
                     EVSQSP"
     gene            14268..14341
                     /locus_tag="VE646_04270"
     tRNA            14268..14341
                     /locus_tag="VE646_04270"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14300..14302,aa:Gly,seq:ccc)
     gene            complement(14366..15037)
                     /gene="nth"
                     /locus_tag="VE646_04275"
     CDS             complement(14366..15037)
                     /gene="nth"
                     /locus_tag="VE646_04275"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HZD79241.1"
                     /translation="MPADRRRVRAIHRRLLRHYGDLEPPRRSDPLEELILTVLSQHTS
                     DVNAERSFQALRGRFATWEDVAEAPSDEVAEAIRSGGLAEVKAPRIQAILRCIAEREG
                     AFDLSALRDRPDRVVREYLTTLPGVGPKTAAVVMAFSLSRATLPVDTHVHRVSRRLGL
                     VPPRASAERAQDLLEALAPDELRVELHVGLIRLGREICRAPSPRCRACPLNDVCPTAP
                     RFLHT"
     gene            complement(15068..16417)
                     /gene="dnaB"
                     /locus_tag="VE646_04280"
     CDS             complement(15068..16417)
                     /gene="dnaB"
                     /locus_tag="VE646_04280"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HZD79242.1"
                     /translation="MRTADEVRRLRPDRVPPHNAEAEESVLGSMMLSADAIADVVEVV
                     RPDDFYHSANAKIFQALREVYGRGDPVDAITAVEELKRRAILDEVGGHLYIHELVEQV
                     PTPAAAASYARIVAETALLRRLISAAADIMDMAYSAPEEPERVADQAEQRIYEVARHD
                     EKEQVASLRELVDQAMEDLERIQNRESAFAGLPTGFRDLDSLLSGMQAGNLIVVAARP
                     GIGKSSFVTNLARNVAVEQGGPVAMFSLEMSRWEIGMRLLCAEARVPWDSIRNKRVGA
                     DDWSRIAQAADVLHEAPLYIVDSGNVTLVDIRAKARRLAARRTGLGLIIVDYLQLMSH
                     TRRVDNRQQEIAEISRGLKMLAKELEIPVIAVSQLNRDPERRQDKRPQLSDLRESGAI
                     EQDADVVMFIHRDDADPAKKGLADVIIAKHRNGPTDTIPLTFLPHLTQFRNFAPPTA"
     gene            complement(16779..17228)
                     /gene="rplI"
                     /locus_tag="VE646_04285"
     CDS             complement(16779..17228)
                     /gene="rplI"
                     /locus_tag="VE646_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HZD79243.1"
                     /translation="MKVILQKPVDKLGVPGDVVEVADGYARNYLMPRGLAVKATRGGL
                     KHIDSLKRAHGVRVSKAKAEAELVAQRLIGAPVRVPARVGEEGKLFGSVTAAEVAAHV
                     EEQMGIAVDRHDIHLDEPIRSVGVHEVRVHLFAEVDPVISVEVVAEG"
     gene            complement(17225..17494)
                     /gene="rpsR"
                     /locus_tag="VE646_04290"
     CDS             complement(17225..17494)
                     /gene="rpsR"
                     /locus_tag="VE646_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003949403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HZD79244.1"
                     /translation="MPAKPKRRNKREKDDKGWQKKQKRKVCIFCKDRIEYVDYKDVMT
                     LRKFVSDRGKIRARRVTGNCAQHQRDVAAAVKNAREMALLPYSTR"
     gene            complement(17484..17927)
                     /gene="ssb"
                     /locus_tag="VE646_04295"
     CDS             complement(17484..17927)
                     /gene="ssb"
                     /locus_tag="VE646_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZD79245.1"
                     /translation="MASDNQIIIVGNLTDDPELRYTPNGAAVAKFRVAVNRRYKDEAG
                     NWKDGETSYFTVNAWRTLAENVAESLTRGTRVVVAGRLQMRSWETQEGDRRTVVEIEA
                     DEIGPSLRWATAKVERQSRSGGDWSAPAPVGAGAGMSEETPLDAG"
     gene            complement(18001..18294)
                     /gene="rpsF"
                     /locus_tag="VE646_04300"
     CDS             complement(18001..18294)
                     /gene="rpsF"
                     /locus_tag="VE646_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019174235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HZD79246.1"
                     /translation="MRQYEIMLILPAEADESVVGAAVDRITRVVSQGGGEVGKIDRWG
                     RRRLAYEIDDRTDGYYVVVEFTAEPSVLVELERTLHLADDVVRFKVVVRGEAA"
     gene            18471..19256
                     /gene="panB"
                     /locus_tag="VE646_04305"
     CDS             18471..19256
                     /gene="panB"
                     /locus_tag="VE646_04305"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HZD79247.1"
                     /translation="MTVTVRDLQAWKDEGKRWAMLTAYDFPTAQILDAAGVPVLLVGD
                     SVANNVLGYDTTLPVTMEEMLHHTRAVVRGVGKAMVVGDMPFGSYQPSVEEGIRNAGR
                     FIKAGAHAVKIEGAQVELTHRLTEIGIPVMGHIGLTPQFVNTMGGYRVQGRSEQAANR
                     LSEQAASLEKAGVFSIVLEGVPADVGRVITRDSSVPTIGIGAGPHCDAQVLVVHDLLG
                     INERHPKFVKAYADLRGQIAAAARAFAEDVAAGRFPGEEHSYH"
     gene            19448..20689
                     /locus_tag="VE646_04310"
     CDS             19448..20689
                     /locus_tag="VE646_04310"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HZD79248.1"
                     /translation="MEPRRGIGLGVVLACLAATLGLGLAVKAPCAAGDWTDGRQYTRY
                     CYSDIVPLLSTEQLTGNRLPYLQPCTTNGPGECDEYPVLSMYLMRLAAWMSNGFAGFF
                     YANALLLTIAAAVAVVCLYLLVGSRAVYLALAPTLLLYGFMNWDLLAVALATASTLAY
                     LNRRDVWSGALLGLGAAAKVYPALLLVPFVLGRFRERRPDAGIHLAWAGAGAWLAVNL
                     PFIVAAPRSWWEFFRFNGARPADWDSLWFIGCHRISGSLGCNAPATIGTGTVNVASAA
                     AFVVLAVAVWVLRRRREPGFPRWTFGFPLLVVFLLTNKVYSPQYGLWLLPWFALALPD
                     LRLFALFEAADVAVFLTRFSWFGRFSGTGGWPIGAFEVAVVIRAAILIACVVTWILRR
                     PAPVQAEARGVADRSPADLAA"
     gene            20686..21864
                     /locus_tag="VE646_04315"
     CDS             20686..21864
                     /locus_tag="VE646_04315"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HZD79249.1"
                     /translation="MSATSLTLSARTRRGIRYSLTVFVAVRIGLFLVALVGLELVPPL
                     RPVSVPGWVAHPLPDPGWHNLFTSWERFDGLWFLRIAAGGYRSGDGSAAFFPLYPLAI
                     RAVSWLIGGHPFAASLIVSNVSFAAALCVLYSLTAAERSEERARRAVLFLATFPTALF
                     FFAPYSESLFLLLAVTAFWAARSDRWGTAGIAAALAALTRSIGIILAPALVVEAFLRW
                     RERGRNPVPGIAAGAAPGVATLGYLAFWGLRSGAWLAPVHQQANWERRFSWPWATLVE
                     ATRFAFRYLGNANGGYWAIDWLIVVPALALSVYAVFRLRASYTVYLWGGLLVPLFYIF
                     PGRPLMSMPRFLLPLFPLFWAASDLSERWRLPRGAVAAVGAGGLALLTVLFVNWYYIF
                     "
     gene            21930..22568
                     /locus_tag="VE646_04320"
     CDS             21930..22568
                     /locus_tag="VE646_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD79250.1"
                     /translation="MTIRVAIADDHSLVRQGLRRYLDMAEGIEVIGEASNGAELIDLV
                     DKESPDIALVDIRMPEMDGLEAARQIRDRFQGVGVIMLTAYDDRQFVVEAVRSGARGY
                     VLKARDAEHLIQTVRLVAGGNMVIDPQLVVALAEELSQAKERDRRAETLTAREIEVLQ
                     LLAFGHTNRDIAERLYISPDTVKTHLEHIFEKLGASDRTAAVAEALRRRLIE"
     gene            22693..24822
                     /locus_tag="VE646_04325"
     CDS             22693..24822
                     /locus_tag="VE646_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZD79251.1"
                     /translation="MSDTHSSSRGASAWPISIALVATMGLVAAAYAFAHERIGSQPAL
                     SSLIVAMACALAILASLPLRRAAGTWHSLRFLLGGVAVVGLANLWLLWAEAENMPRYG
                     PSAYDALFLLAAAFFWIPAQLEYRHHFRKDDRREIAADVALISAALGTILYLWIRPDG
                     ATFTSSLSSAVFALIAATAFSSYGALALWVPSVGHAGQFAAVGAFSSALMAFATPWVR
                     GAYRPGTVQVDLPLALGALGLAALLTLAPRSTPSGPITRPTRYGRALLTTVAVAAACA
                     ALGLIAGAESRGEIGNLHATGLIWLLGAAVALRILANQLRSTQDAQAARDALDQKEMA
                     LLETDKALARLQRANETLRESEERLRLVFEAAVDGIVELDEHDVIVRANEAFCQMLEL
                     PQSMVEGQPWSALGAVVQADESFGTLPQTGQGTLMREGQAIYLEARTSDVPGDPPRRL
                     LLVRDVTAARVADQTIRSLFKFLQDRDEDRTRIMRRTNAAIEGERNRIARDLHDGPVQ
                     GVSAASLSLEAVLLMLKAGETEEGVEVLGKVRKELAEEADNLRRLMAGLRPPLLEERG
                     LVPALRENLEKFGHDTGIQTVFHANGVTDVPQDLETLAYRIVQEALSNAGKHAHAGRI
                     SVGVEALGGQLRIEIADDGKGFDSAKAREFLRLGRVGLASMRERVELANGTFMVHSTP
                     GKGTTVLATLPLESVPGPREFAVNEAF"
BASE COUNT         3509 a         8838 c         8556 g         4075 t
ORIGIN      
        1 agagcaggac gccactgcca ccggccacgg tgagcaccac gaacgcgatc cggatcagga
       61 ccggatcgac gcccaggtac catccgatgc cgccacagac gccggcgatc acccgatcgg
      121 accggctgcg ccgcaaggag cgcgtcggtt ggcgcgaggg cggagggggc ggtggcggtg
      181 gcggcacgga cacgccgaaa ccctagcgcc tggggcgctc ccctccaagc ccgaaggcgc
      241 cctctcaggc gggctgatgg gcccggaata ccaggccgtc cggccccgcg tccacctcga
      301 cccggtcacc ctcccgcagc tcgccggaca gcagcttcat ggccagggca tcctggatct
      361 cgcgctggat cagccgcttc agcggacggg ctccgtaggc gggctcgaac cccttgtccg
      421 cgaggaggtc gcgggccgca tccgtcggct cgaaggtgag gttccgctcg gacaggcgcc
      481 gctgcagctg gccgagctgg atgtccacga tctgggcgat ctcctcgcgc cccagctgat
      541 ggaagacgac gatctcgtcg acccggttga cgaactcggg acggaaggtg gcccggacct
      601 cggccatcac cttctccttc cgctcctcga tcgatgccat cgggtccgcg aacacgtacg
      661 agcccaggtt cgaggtcatg atcaccaccg cgttgcggaa gtccacggtc ctcccctgcc
      721 cgtcggtcag ccgcccatcg tccaggagct ggaggaggac gttgaaggcg tcggtgtggg
      781 ccttctcgat ctcatccagc agcaccaccg agtaggggcg gcggcggacg gcctcggtga
      841 gctgaccgcc ctcctcgtac cccacgtacc cgggcggcgc accgatcagc cgggagaccg
      901 tgtgccgttc ctggtactcg ctcatatcga tccggaccat ggcccgctcg tcatcgaaca
      961 ggaactcggc cagcgcccgg gccagctcgg tcttccccac cccggtggga cccaggaaga
     1021 tgaacgaacc gatcggccgg tccgggtcag acagccccgc ccgggaacgc cggatcgcgt
     1081 tggccaccgc ctccacggct tcgtcctggt ccacgatgcg ccggtgcagc gcgccctcca
     1141 ggtggaccag cttgtccatc tcaccctcca tcagacgggc cacggggatg ccggtccact
     1201 tggagacgac ctcggccacg tcctcctcgt cgacctcctc cttcagcatc ttccgatcgc
     1261 gctgcagctc ggccagctcg gtgttggcct cctccagacg cttcgtgagc tcgaccagcc
     1321 gcccgtaccg aagctcggcg gcccgctcga gctcgccgtc gcgctcggct cgctcggcct
     1381 ccatccgggc ctgctcgatc tcggacttca gggagcggat ccggtcgatc tgctccttct
     1441 cccgccgcca atgcgccttc atgccctcgg cctgctcccg gaggtcggcg agctcggcct
     1501 cgagccggtc ccggcgctcg gcggacaccg gatcggtctc cttcttcagc gccgcccgct
     1561 cgatctcgag ctgcttgatg cgccgctcca cctcgtcgat ctccaccggc atcgagtcga
     1621 tctcgatccg cagccggctg gccgactcgt cgatcaggtc gatcgccttg tccggaagga
     1681 accgtccggt cacgtaccgg ttggacagga ccgccgcggc caccagggcc gagtcctgga
     1741 tccgcacccc gtggtggacc tcgtaccgct ccttcaggcc gcgcaggatc gcgatcgtgt
     1801 cggccaccga gggctcgccc acgtatacgg gctggaaccg ccgctccagc gcggggtcct
     1861 tctcgatgtg ctggcggtac tcgtccagcg tggtcgcccc gatggcccgg agctccccgc
     1921 gcgccaacat cggcttcagc atgttgccgg cgtcgaccgc tccctcggcg ccgccggcac
     1981 caacgatcgt gtgcagttcg tcgatgaacg tgatgacctg tccctcgctc tcggcgatct
     2041 cgcgtagcac ggccttgaag cgctcctcga actccccccg gtacttggag ccggccacca
     2101 tcgccccgat gtcgagcgcg accacccgct tgtccttcag ggactccgga acgtcgccgt
     2161 ccacgatccg ctgcgccagg ccctccacga tggccgtctt gcccaccccc ggctcgccga
     2221 tcagcaccgg gttgttcttg gtccggcggg agaggacctg gatcacccta cggatctcgt
     2281 cgtcgcgacc gatgactggg tccagcttgc ctcggcgcgc cagctcggtg aggtcgcgac
     2341 cgtagcgctc cagcgcctgg tacttctctt cggggttcgg atcggtcacg cgctggcggc
     2401 cccggacctc ggccagcgcg gcgagcgccg cgtcccgcgt gatcccggac tcccggagga
     2461 cgcgggccac gttcgagtcg cccctcaaca tcgccaggaa caggtgctcg gtggacacgt
     2521 actcgtcggt gagcgcctgt gcctcgcgga gcgcctcctc cagcaagctc gcggtggccg
     2581 aagacacacg cacctcggcc gcaccctggg cgtagacctt cgggatccgc tccaacagcg
     2641 cctcgatgcg gtcccggagc gcacggggcg acgttcccag ccggtgcagc agcgggtaca
     2701 cgacaccctc ggggtccgac agcagggcga acaggacgtg ttcgggctcg atcgcctggt
     2761 ggttccgggc gagcgcctgt tcgcgggcag cctccagggc ggcctgcgac ttcatggtca
     2821 gcttctgcag gtccatcggt gatccaccgt ctccatcttc tcggtcatgc tcggtcatgt
     2881 cgtcgtgcgc gctcgtgccg gggcgggctc gcagggccgc cctcaggtga ccacccggtg
     2941 gccggcccag gggaaggaca cgacggtccg gaccggcacg atgtccaccg tcgcgctgga
     3001 cgggccgagc ggcaccggcc gccccctgcg cacgcgccgg agctcgctct cgagctcggc
     3061 ggtgcgccga cgcaggtcct cgagctccgc ctgcatctcg atgatcatct tcgccccggc
     3121 cagattgacc ccttgctcct gggtcagctc ctggatcagc tgtaggcgcc ggatgtcacg
     3181 ctccgagtag cggcgggtgt tgccagaggt gcgcttgggc cgcaccagac ccttgcgctc
     3241 gtagatccgc agggtctgcg gatgcacccc cgcaagctcg gcggcgacgc tgatgatgta
     3301 gacggcacga tcctcccgtc ggtccgcttg cccttgccgc ttcatcccgt cgtcgccccc
     3361 tcggtcacgc atccactccg agccgcttgc gcggccgatc cttctccatc tctttcagtt
     3421 gccgcagcag ctgcttctcc tcccgagaca gcttcctcgg gacctcgatc tccaccctgg
     3481 ccagcaggtc accgtgtccg cccttcttgg gcgcgcccct gccccggatc cggaaggtcc
     3541 gtccgccctg ggtcccggca ggcaccttca gcgtcacggg accgttcagg gtgggaacct
     3601 ccacgttcgc ccccagcgcc gcctccgcgt aggtcaccgg caccgtgatg gtcaggtcgt
     3661 tccccttgcg tccgaacagg gcatgggacg cgacgtgcac caccacgaac agatcgccgg
     3721 gcttcccccc ggcgggaccc ggctccccgc gtcccgccag acggatccgc gccccgtcct
     3781 tcacgccggc cggcaccttg acgttgaacc gacgcgtgct ccggatgctg cccgagcccc
     3841 ggcactgcgt gcaggggtgc tccacgatgt ggccgctccc gtggcaccgg gggcaggtct
     3901 gcgtcatctg gaagaagccc tggttcacgc tcacggcccc ggcgcccccg cactgggggc
     3961 aggtcaccgg cgagctcccc ggctcggcgc cgctgccgcc gcagcgggga cacggagccg
     4021 gaccctggat ccggaccggt accgtggtcc cacgcatcgc gtcctcgaag gagacccgga
     4081 cctcggtctc caggtccgcg ccccggggcc gcccggtgcc cgtgcgtcgg ccgccacccg
     4141 tgaagacgct gaacaggtct ccgatgtccc ccatgccgcc gaagccgacg tcctcgaacc
     4201 gaacccggcc gcccgcgccc cccggaccgg cgcccgggaa cccacctggg ccgtagccgg
     4261 aggaggccat ctcgcggacc tggtcgtact gcttgcgctt gtccgcatcc ccgacgacgt
     4321 cgtaggcggc cgagatctcc ttgaaccgct cctcggccgt ctcgttccca gggttcgcgt
     4381 ccgggtggta cttctgcgcg agcttgcgat aggccttctt gatctcgtcc tggggagcgt
     4441 tcttggcgac gccgagaacc tggtagtagt ccttctcgaa ccactcacga cgcacgtcgc
     4501 cgttcatggc gacccctcag gcctcgtgcg tcacacggac gcccgacggg cggatgaccc
     4561 ggtcccgaag cgtgtacccc tgtcggagga catcgcccac gaccagatcc ccctcgcccc
     4621 ccgccgagcc catcagggcc tcgtgcagct ccgggtcgaa cggcttgccc tcggcctcga
     4681 tccgctccag gccctccgcc ttcagcgtgt ccatcagctt ggcgtacacg agctccacgc
     4741 cgtgcaggaa cttgtcgaag tcgggcttct gctcggcggc catcagggca agctcgaact
     4801 cgtcgatcac ctcgagcagg cggcggatca ggggctcggc cgccatctcg acggcgcggg
     4861 tctgctcctt cagcacgcgc ttgcggtagt tctcgaactc ggcctgcagg cgcaccaggt
     4921 gatcgcggta ggccgccgcc tcctgccgtg ccgcctcgag atccacggtc cccgaggcct
     4981 cggtggtcga tccggaggtc ggggcctcgg gggtgggcgc cagtccctcc tcctcggcgg
     5041 gatcggccgg gggggccgcg ccggcctcgg ccggcgcgga ccctccctcg tcccggaccc
     5101 gtcgcttgtc ggtcaccctc acgcgcgggc ggtccatctg ctcgcgatcc tcgcgatcgt
     5161 gctcggtcac ttggccttca ccgtgatctt cttcggcttg gccttctcga tcttcggcac
     5221 ctcgacggtc agcacgccct tgtcgaagga cgcctcgacc ttctcggtgt cggccgtctg
     5281 cggcaggccg atcgaacggg tgaacgcacc gtaccgccgc tcgatgcggt ggtagtcctc
     5341 ctcgttcacc tcggactcga actcccgctt gccgctgatg atcagggtgg agtcctccac
     5401 agagacgtcc actgcgcccg gatcgatgcc cggcagctcg agcttggcca cgaagcggtc
     5461 ctcggtctcg tacacgtcca gcgccggcat ccacgtcccg gcgccgaccg cgcgcgtggc
     5521 ctcggccccc gcgaaggtcc gtccgaacag gcggttcagc tcatcctgga tcgagaccag
     5581 gtcacggaac ggatcccagc gaaccagcgt ctccctcact tggcacctcc ttcatcgacg
     5641 atctcaccct cgacgacgtc ctcgtcgccg ccttcggaag cagcttcccc accgcccgcg
     5701 gcggcggcct gctggcccgg ctcagcctgg gcctgcgtgg tctggtacat ggcctggccg
     5761 atgcgctgga aggcctgcat cgcggcatcg gtggccgacc tgatggtgtc gacgtcttcc
     5821 cccctgagcg cctccttcag ctcgtcgttc gccctggtca actggtcacg atcgccctgc
     5881 gggaccttgt ctccccactc ctcgatcgcc ttgtcgatct ggtaggtgag ctggtcggcg
     5941 ctgttccggg cctcggcagc ctcgcgccgc cggtggtcct cggccgcgaa cttctccgcg
     6001 tccttgacca tgtgatcgat gtcgtccttc gacagggcgg tgccgccggt gatggtcatc
     6061 tgctgctcct tgccggtggc gaggtccttg gccgacacgt tcacgatgcc gttggcgtcg
     6121 atgtcgaagg cgacctcgat ctgcggcatg ccgcgcggtg ccggcgggat ccccatcagg
     6181 tggaacttgc ccagcgagcg gacgccgggc gagtacacgg tctcggcctc gccctgcagg
     6241 acgtggatct ccaccgtggt ctggccgtcg tcggccgttg tgaagatctc gctcttgcga
     6301 gtggggatgg tggtgttgcg ctcgatcagc ttggtgaaga tcccaccctt ggtctcgacc
     6361 cccagcgaca ggggcgtgac gtcgaggagc aggatgtcct tcacgtcgcc cttcagcaca
     6421 ccggcctgga tggcggcacc gaccgagacg acctcgtcgg ggttcacacc cttgtgcggc
     6481 tccttgccac cggtcagctt cttcaccagc tcctggatca tgggcatgcg ggtggccccg
     6541 cccaccagga tcacgtggtc gatcgcggac acgtccttgc cccagtcctt gacggcctgg
     6601 tcgaacgggc ttcggcagcg ctccacgagg tcctcggtca tgcgctcgaa ctccgagcgc
     6661 gtgagcttca tctccaggtg cagcggacct tcggccgtag cggtgatgaa cggtaggttg
     6721 atggtggtct cggccacctg ggacagctcg atcttggcct tctccgccgc ctccttcagg
     6781 cgctggaggg ccatccggtc ggcgcccagg tccacgccgt gcgcgttctt gaactccttg
     6841 accatccagt cgatgacgcg ctggtcccag tcgtcgccac cgaggtgggt atcgccgtgc
     6901 gtggccttga cctcgaacac gccctccccg atctccagca gggagacgtc gaacgttccc
     6961 ccaccgaggt cgaagacgag gatggtctgg tcgtgctgct tgtccagacc gtaggcgagc
     7021 gcggccgccg tcggctcgtt gatgatgcgc aggacctcca ggccggcgat ctgtccggcc
     7081 tccttggtgg cctggcgctg cgcgtcgtcg aaatacgcgg gcaccgtgat gactgcctgg
     7141 gtcgcggtgt cgcccaggta cgcatcggcg tcccgcttca gcttggtcag gatctgtgcc
     7201 gagatctcct gcggggtcca cttcttcccg tcgatctcga ccgaccagtc ccgctcgccc
     7261 atgtggcgct tgaccgaccg gatcgtccgg tccgggttgg tgatggcttg tcgtttggcg
     7321 acctcgccca ccaggatctc gccgttcttg gagaacccga ccaccgaggg ggtggttcgg
     7381 gagccctcgg cgttcgggat gaccaccggc tcgccggcct ccagcacgga gaccaccgag
     7441 ttggtcgtcc cgaggtcgat tcccacggca cgtggcatga gcatcctcct cgtcgtctta
     7501 ctgagtcgat agcgaggata aagcttgagt gtatgagtgt caaagtcgag ctcaacgatc
     7561 ccgcttggcc agcgaggata ggcatcgacg ggggctcacg gaggatctcg ggatctcctc
     7621 aacctacgga cgtgccagag ggggcggggc gagcccccgg acctcggggg gtagactcgc
     7681 gagcatggcg gcccggatcg tcgcgctgct cgccctgctg atggtggcgt tcggactgtt
     7741 cgcccgccgg gtcgccctcc tggcccgcct ggcccgcctg ggcaggccgg tcgagcgcgc
     7801 ccgagacgtc ccgacccggg tggagcggga ggtcaccgtg gtcttcggcc agcgcaagct
     7861 cctgcaacgc ctcgtgccgg gcctgatgca cgcgttcatc ttctggggat tcctggtcct
     7921 gctgaccacg atcgccgagg ccatcgggca ggccgtctcc cctacgttcg cgctcccggt
     7981 cctgggccac gccgcctggc tggggttcgt gcaggacctg ttcgccgcgt tggtggtggt
     8041 gggcatcgtc atggcgctcc tgatccggaa ggtgcagcgg cccgagcggt tcgtggggag
     8101 ccatctgcgc gaggcggacc tgatcctggc ttggatcttc ggcgtggtcc tcacgttgtt
     8161 cggactgaac gccacccgca tcgcgcaggg cctgatcgag tcaccagcgg cctggatgcc
     8221 gatctcgaac gccctcgccg ccctgttccg tccactcgcc ccatcgaccc tgcagacgct
     8281 cgcctgggcc ttcctctgga gccacctggt cctggtcttc ggattcctgg tcttcgtgcc
     8341 ctactccaag cacctgcaca tcttcgtcag cttccccaac gtcttcttgt cgaacacgaa
     8401 gccccgtgga tacctgaccc ccctgcggat cgatctggag gccctcgaga ccgaggacgt
     8461 ggggctcgga gcggccagca tcgaagacct gtcctggaag cagatgctcg atctctacgc
     8521 ctgcaccgag tgcgggcgtt gtcagggcgt ctgcccggca tggaacaccg gcaaaccgct
     8581 gtcccccaag ctgctgatca tgaacctgcg ggaccatctg ttcgagcagg gaccgaagat
     8641 cctccaggcc cgagccgatg gcaccgagct agctgcggtg gctctgaacc ccgacgtggt
     8701 agacgacgag gtgctctggt cgtgcacgac gtgtggggcc tgcatgcagg agtgcccagt
     8761 ggacatcgag cacatcgacc acatcgtgga catgcgtcgg agcctggtga tggccgagtc
     8821 ccgcttcccc caggaagccg gcgccctgct caggaacctg gagggagccg gcaacccttg
     8881 gggccaaccc caggacgaac gggcggcatg ggcgagcgac atggacgtcc cggtcgtcgc
     8941 cgatggcggt ccaccgttcg agtacctgta ctgggtggga tgtgccggct cgttcgacga
     9001 tcgggccaag gccatctccc gagcggtcgc caccctgctc cgacgggcgg ggctgtcctt
     9061 cgccatcctg ggcgcgcagg agcggtgcac gggtgacccc gccagacgga tgggcaacga
     9121 gtacctcttc cagaccctcg ccgagcagaa cgtcgagatc ctgaaccggg cgggggtcac
     9181 gaagatcgtc gccaactgcc cccactgcta caacacgctc ctgaacgagt acccggacta
     9241 cggagggcgg ttcgaggtca tccatcacac gcagctcttc cgtcggctcg tggccgaggg
     9301 tcggctgcgc ccatcggccg gggtccggag cctcgtgacg taccacgacc cctgctacct
     9361 gggccggcac aacgagatct acgccgctcc caggcagctg ctggacgggg tgccgggcgt
     9421 ggccacagtg gagatgccgc gtcacggtcc ccgcgggctg tgctgcggag cgggcggggc
     9481 ccgcatgtgg atggaggagc ggatcgggaa gcggatcaac gaggagcgga tggacgaggc
     9541 cgcctccacc ggtgccgaca cggtgggggt agcgtgcccc tactgtctgg tgatgctgga
     9601 cgacggcgcc cgatcccggg gagagggaac tgaggtcgcc gacgtcgccc agatcctcgt
     9661 tcgggcggtc gagcccggcg actaggccct tcggcgtacc acccgggccg ccggtgggtc
     9721 gctcacggac gacaagcatc gtgctcgatg gtgagcccgg gccatcccgc ctcctcatct
     9781 ccacgctccg cggtcaaggg cggcagcgga ccatccgaaa gaaggactag accgaacggc
     9841 tcgggaccgc ccatcggaca cccccgggtc gagagccgta tgcgggccac gcgaacgaac
     9901 gagcgtgtcg acggagggac cgcgggatgg acacgatccg ggatcgggcc gatcgagcgg
     9961 cggaatgccg ccgcctggcc gagcagctcc aactcgatgc ggtcggcatc gtgcgctccg
    10021 gtccggatgg gcgtgagctg tcctggtggg ccgcccccgg agggccaccg cgacccctcg
    10081 acgtggacga ggtgctgacc ggtcacgccg cggggtgggt ggtgtgtccc cggagcgacg
    10141 aggtggtgtt cggtcgtctg acgccggaga gctcggtccg ctcggtccag gtgttacggg
    10201 ccatgctctc cgatctgtcg ggtgaacccc cgcgaaccgc cgccccgggc gccgcgtcgc
    10261 cggacgaccc ggtggctcgc gaacgtgctc gatgggccta cgcgatccac gatgggctga
    10321 cgcaggtggt caccgcggcc gtgctggacc tggagtggag ggcacgcaag ctcgagctcg
    10381 agccgggaga ggcgatcacc gccctgtcag aagccgcctc cgagctccac agcgccctgg
    10441 aggacgtacg gggcgtgctg gcgatcctga ccccgccgga gccgctggag gaggagtccc
    10501 tggaccactt ggtggaggag ctcttcggac ggtggcagct gccctcatcg tggagcatcg
    10561 agggagacct cgattcgatc ccccggccga tcctcgaggc cgcctcctcg atcatccgcg
    10621 agagcgtggc gaacgccgcg aagcactcct cgacgcagga ggtgaacgtc cggatccagg
    10681 cgtccggcca ggagatggag gtgagcgtgg aggaccgtgg gcggggtttc cgtcctacgg
    10741 agacggggct gcacgccggg cacctgggac tggagatgat gcgacgccgc gttgccgagc
    10801 tccatggctc gctcgacatc cggtccgagc ccggtcatgg cacacgggtg gtcgctcggc
    10861 tgcccgtcgg ggaacaagga gacgaaccgt gaggatcatg atcgtcgacg accacgccct
    10921 ggttcgccgc ggcatggccc acgtcgttcg cgaatgcttc gccgacgcgg acgttgtgga
    10981 ggcggccgat gccgctgaag ccatgggcgt gatggagcgg accccggccg acatcgcgtt
    11041 ggtcgacgtc cggctgccgg tgcacgacgg cctcgagctg ctgcacgaca tgaagtcccg
    11101 ctggcgcgag gtgcccgtga tcatgctgac caccttcgat cacgctcagt acgttcgccg
    11161 ggcgctggcg gagggagcgg ccgggtacct gctgaaggac gccacccccg aggacctcga
    11221 gcaggccatc agggtggccc tatcgggtgg agggaacgtg ctgtcccctc gggtgatcca
    11281 gaacctgttc gaggcgatgg agacgggtga gggggaggag gaggctccgc accgccggcc
    11341 ggccgccaac ctcacacagc gcgagacgga catcctggag ctccttgccg agggcaagag
    11401 caaccgagac atctcccggt cgctgttcct gtcggagaag accgtgaagg cccacctcgc
    11461 cgccatcttc cgcaagcttg gggtgagcaa ccggacccag gccgccatgg cagcggtctc
    11521 catgggcatc ggctcggcac acttcccggt cgaccgagcc gaggccgtgg gatccggggc
    11581 cggcgatcgc tcgtgagcgg agcgaaggga cgatccggct cgcggggacc ggaggaacga
    11641 cgtggcggag agcgaaggaa cccgatccaa cggcgaccgg gggaccacca cggctgaggc
    11701 aaccggggcg gtggggccgg gcgggttcgg ccatcccggt ccggtcgagg cctacgagga
    11761 ggactggtat cgaagcctga aggccctcgc ggaacgccga cgcgacctcc tcggggagga
    11821 gagggacgct cccgagggtc cgaccggacc cttctcggag gagccggtcg ctcactccga
    11881 ggccaccgag ggccccgtcc aagatcccga cgacgagatc cagacggtcg cccggcagga
    11941 ggccgagcga gcaggggacg agctcgcgca gcgggaccgg gcggtccgcg ccctcggtca
    12001 ctgcacccag ggtctcccgg ggcctgccat ggagctggct ctggccgatc ccgatcccga
    12061 cgtacgagcg acggccctgc agtggttgcc cccgatgccc gagcgggggc tggcggacat
    12121 ggtctcccgg tcgctcggcg acccggacga gcgggtccgc cgggcggcga tgcggcggtt
    12181 cgccgagtgt gccggtgccg accgagacgc ggcgtggaac gcgctacgca cgtgcccggc
    12241 gaaggagctg ggcgacatgg tggcggcgct ccagcagacc cagacagcct cgatcgagct
    12301 cgcgttcgag cggctcagct cgtcggaaga gggcgagcgg gtcctggcaa tcgaggtggt
    12361 gggatggggc aggtccccgg gttgcgtgga ggcggcggtc catgccctgg gggacccttc
    12421 ggcaccggtc cgccgcgccg cggtggcatc gctcggtcgc ctccgcgacc cctccgcagc
    12481 cgtggcactc aacaaggcgc tggtggaccc ggatccggat gtgcgggtgg gcgccgtgaa
    12541 cgcgctcggt gtgatcgacg acgagtcggt gctgggatcg ctcgtcaccg ccctcaagga
    12601 tcccgagccc cgggtccggg agatggccag cgaggtcctc accgagtgga gctcccccgc
    12661 cgtggcacgc cggctggcgg aggtgctggc cgtaccgagc cagcgggact cggcggccgg
    12721 cctgctgatg cggatcggac ccgcctccct ggaactgctg atcgacgtcc ttcgtcaagg
    12781 caaccaggtc ctcaccccca tcgtgggcgg gctgctccaa cggatcgtcg ggttcgagga
    12841 cctgctcggg cggttcagct cacccgatcc cgaccgccgg ctccgggccg tggaggcggt
    12901 ggccgccatc ggcggctcgc cggcggtgga tgcgctgatc ggcatgctgt ccgaccgaga
    12961 cgagcggatc cggatccgtt cagcgcaact gctgggggcc ctcggtgatc gacgcgccga
    13021 gcagcccatc cggcaggcgg cgctggaaga tcccgttccg gaggtggtcc aggccgccgc
    13081 cgagaccctg gctcgcctgg gccccgagga cggcggccgg gccgcctgag cctacgggct
    13141 ctgggaaacc tccacccacc gctccaggat ctggccggcg gcgcccgagt cgatggaagt
    13201 cgcggccagc tccatcccct gtgcgaggga atcggcacgc ccggcgacct ccagcgcggc
    13261 gcccgcgttc agcaggacca cgtcacgggc cggtcccgcc tgcccgtcca ggacgcgcct
    13321 ggcgatcccg aggttctcgg ccacttcgcc cccccgcagg tcctccgggg tcgccggggg
    13381 caatccgagc tcaaacgggt cgagcgttcc ttccgacgcc gaaccgtcac ggacccggaa
    13441 cacggtcgag ggacccgtcg tggtgagctc gtccagaccg tcgagcccac gaaagaccag
    13501 ggcccgcacg cgccgccggg ccagcacctc ggccaccagc gggagcatgc gggcatcgga
    13561 cacgcccacc acctgcgccg cgggacgtgc cgggttcgtg aggggtccga ggaagttgaa
    13621 gatcgtgggc acgcgcagct cccgacggat gccggtggcg tacgccgtgg ctgggtggaa
    13681 gaccggcgcg aacatgaagc cgatgcctgc ctccgcgaga caccgctcca ccccctcggg
    13741 ctcgtggtcg atcttcacgc ccagggcctc gagaaggtcg gccgatccgc agcgggacga
    13801 cgccgcccgg ttcccgtgct tggccaccct cacgccggcg ccggcgacca cgagcgcgct
    13861 caccgtggag atgttgaagg tcgaggcccg gtcgccaccg gtgccgcagg tatccaccag
    13921 gctgccccgg aacgtgaccg gtcgggcgaa ctcgagcatg gcccgggcga ggccctcgac
    13981 ctcgtcgacc gtctcgccct tcgtgcgcag ggccatcagg aagcctgcga cctggaccgg
    14041 tgtagcctcc tcccgcatga tcgagcccag ggcctgcgag ctctcggcgg ccgacaacga
    14101 cccgcccccg atcagccgag agaggatcga cggccacaga tccggatccg gcatggagcg
    14161 gacgctagca ggtcgctccc ggcggcgacg agccggatgc ggtgggccgg acgagaacag
    14221 ctccgctcgc tacacttgcc tccgcggccg gcctgccgag ccgttccgcg ggcgtggttc
    14281 aatggtagaa cgcgagcttc ccaagctcga aacgggggtt cgattcccct cgcccgctcc
    14341 atccattcgc ccccccgctc ccgcctcagg tgtggaggaa cctgggtgcc gtcgggcaga
    14401 cgtcgttcag cgggcaagcc cggcatcgag ggctgggcgc ccggcagatc tcgcgaccga
    14461 gccggatcag tcccacgtgg agctcgaccc ggagctcgtc cggggcgagg gcctcgagca
    14521 ggtcctgggc ccgctccgcg gacgccctgg gcggaaccag cccaagcctg cggctcaccc
    14581 gatgcacgtg cgtgtccacc ggcaacgtgg cccggctcag cgagaaggcc atcaccacgg
    14641 ccgcggtctt ggggccgacg cccggcaggg tggtgaggta ctcgcggacc acccggtcgg
    14701 ggcgatccct cagcgcggac aggtcgaagg ccccctcacg ctccgcgatg caccggagga
    14761 tggcctggat cctgggagcc ttgacctcgg ccaggccgcc ggatcggatc gcctccgcca
    14821 cctcatccga gggagcctcg gccacgtcct cccacgtcgc gaaacgccct cggagcgcct
    14881 ggaacgatcg ctcggcgttg acgtcgctgg tgtgctgcga gagcacggtg aggatcagct
    14941 cctcgagcgg atcgctgcgg cgtggcggct ccaggtcgcc gtagtggcgg agcaaccggc
    15001 gatggatcgc tcgcacgcgg cggcggtcgg caggcatccc gccagcgagc ctacgcgatg
    15061 ccccggctca ggcggtgggc ggtgcgaagt tccggaactg cgtcaggtgg ggcaggaacg
    15121 tgagcgggat ggtgtcggtg gggccgttcc ggtgcttggc gatgatcacg tcggccaagc
    15181 ccttcttggc ggggtcggcg tcgtcgcgat ggatgaacat caccacgtcc gcgtcctgct
    15241 cgatggctcc ggattcccga agatcggaga gctgagggcg cttgtcctgg cgccgctcgg
    15301 ggtctcggtt caactgggac acggcgatca ccgggatctc gagctccttg gccagcatct
    15361 tcagcccccg gctgatctcg gcgatctcct gctggcggtt gtccacgcgg cgggtgtgtg
    15421 acatgagctg gaggtagtcg acgatgatga gtcccaggcc ggtccgtcga gcggccaggc
    15481 gccgagcctt ggcgcggatg tccacgaggg tgacgttgcc cgagtcgacg atgtacagcg
    15541 gcgcctcatg gagcacgtcc gcggcctggg cgatccggga ccagtcgtcc gcccccaccc
    15601 gcttgtttcg gatgctgtcc cagggcaccc gcgcctcggc gcagagcagc cgcatgccga
    15661 tctcccagcg cgacatctcc agggagaaca tcgcgaccgg gccgccctgc tcgaccgcga
    15721 cgttgcgcgc caggttcgtg acgaacgagg acttcccgat gcccggacgg gccgccacca
    15781 cgatgaggtt gcccgcctgc atccccgaca ggagggagtc caggtcgcgg aagcccgtgg
    15841 ggagtccggc gaacgccgac tcccggttct ggatccgttc caggtcctcc atcgcctgat
    15901 cgaccagctc ccgcagcgag gcgacctgct ccttctcgtc gtggcgggcg acctcgtaga
    15961 tccgctgctc ggcctggtcg gcgacacgtt ccggctcctc cggcgccgag tacgccatgt
    16021 ccatgatgtc cgcggccgcc gagatcagcc ggcgaaggag cgcggtctcg gcgacgatgc
    16081 gtgcgtatga ggcggcggcc gcgggcgtgg ggacctgctc cacgagctcg tggatgtaga
    16141 ggtgaccccc cacctcgtcc aggatcgccc gccgcttcag ctcctcgacc gccgtgatgg
    16201 catcgacggg atcgccgcgc ccgtacacct cgcgcagggc ctggaagatc ttcgcgttgg
    16261 ccgagtggta gaagtcgtcg gggcgcacga cctcgaccac gtcggcgatg gcgtccgccg
    16321 acagcatcat cgagccgagg accgactcct ccgcctcggc gttgtgcggt gggacccgat
    16381 ccggccgaag acgccggacc tcatcagccg tgcgcaccca ggaacccccc tcgagggatg
    16441 atcagcagcc ggagcggaag gctagacgga ggcaggcgcc gagtcattgg gataagccct
    16501 gtggattcag ggatcccagg tcgcccaggg agatttgttg tcacacccgt cctcacctgc
    16561 gcctccgctt gtggaaaagc gcttcagaac gtgtgtgtac acgacgggga tgacacgcat
    16621 gtgtttcccc agctagacgg ggtcgagctc gggaccaccg tcggcgttcg cgcgaggcac
    16681 cgtggccagt acagaagggt tccggggccg ggaggaggcc tggtgagcgc ccgtcggctc
    16741 agccgcgagc gagcgagacg cccgcccggc ccctccccct acccctccgc gacgacctcg
    16801 accgagatga cggggtcaac ctcggcgaac aggtgcacac gcacctcgtg gaccccgacg
    16861 ctgcggatcg gctcgtccag gtggatatcg tggcggtcca ccgcgatccc catctgctcc
    16921 tcgacgtggg cggcgacctc ggccgcggtg acgctcccga agagcttgcc ctcctcaccg
    16981 acccgagccg ggacgcggac gggcgcgccg atcagccgct gggccaccag ctcggcttcc
    17041 gccttggcct tcgacacccg aaccccgtgg gcccgcttca ggctgtcgat gtgcttgagc
    17101 ccacccctcg tggccttcac cgcgagcccc cgcggcatga ggtagttacg cgcgtagccg
    17161 tcggcgacct cgacgacgtc cccggggacg ccgagcttgt cgaccggctt ctggaggatg
    17221 accttcatcg ggtcgagtac gggagcagcg ccatctcgcg ggcgttcttg accgctgcgg
    17281 caacgtctcg ctggtgttga gcgcagttcc cggttacccg ccgggctcgg atcttgcccc
    17341 gatcggagac gaacttgcgc agggtcatca cgtccttgta gtcgacgtac tcgatccggt
    17401 ccttgcagaa gatgcagacc ttgcgcttct gcttcttctg ccagcccttg tcgtccttct
    17461 ctcgcttgtt ccttcgcttc ggcttagccg gcatccagcg gtgtctcctc actcatccca
    17521 gcgcctgcgc caaccggggc cggagccgac cagtcaccgc cggaacggct ctgtcgctcg
    17581 accttggcgg tcgcccaccg caggctcggt ccgatctcgt cggcctcgat ctcgacgacc
    17641 gtgcgccgat caccctcttg ggtctcccag gagcgcatct gcaggcgccc ggccaccacc
    17701 acgcgggttc cccgggtcag ggactccgcc acgttctccg ccagggtgcg ccaggcgttc
    17761 accgtgaagt acgaggtctc cccgtccttc cagttgcccg cctcgtcctt gtagcggcgg
    17821 ttcaccgcca cccggaactt ggccacggcg gcgccgttcg gcgtgtaccg cagctcgggg
    17881 tcgtcggtca gattgcccac gatgatgatc tggttgtcgc tcgccatctc catcacctac
    17941 ctgtcgtgtc cgcttcccga cggatggagg gccgggaagg gggaagtcgc cttggtcgcc
    18001 tcacgcggcc tcgccgcgga cgaccacctt gaaccgcacc acatcgtcgg ccaggtgcag
    18061 cgtgcgctcg agctccacga gcacggacgg ctccgcggtg aactccacca cgacgtagta
    18121 gccgtccgtc cgatcgtcga tctcgtaggc caggcgcctg cgaccccacc gatcgatctt
    18181 cccgacctcg ccaccgccct gcgagacgac ccgggtgatg cgatcgaccg ccgcgccgac
    18241 gaccgattcg tcggcctcgg cgggcaggat gagcatgatc tcgtactgcc tcagttctcc
    18301 acctcctctg gtctggacgg cccccgagag ggagcaggag tgggacctcc agagtgtacc
    18361 ggctgcacgt ggaccgtaac acggagcacc gacaccccac gcccccgggt ccggaccgag
    18421 cggtagactg cgcggacagc cgtgcactcg aacggaagga ggcgcgagcg atgacggtga
    18481 ccgtccgcga cctgcaggcg tggaaggacg agggcaagcg ctgggccatg ctcacggcct
    18541 acgacttccc cacggcgcag atcctcgatg cggctggggt cccggtgctc ctggttggtg
    18601 attccgtcgc gaacaacgtc ctcggctacg acacgaccct gcccgtcacg atggaggaga
    18661 tgctccacca cacccgggcc gtggtccggg gcgtcggcaa ggccatggtg gtgggtgaca
    18721 tgccgttcgg ttcgtatcag ccttcggtcg aggaggggat ccgcaacgcg ggtcggttca
    18781 tcaaggccgg cgcgcatgcg gtgaagatcg agggggccca ggtcgagctc acccaccgcc
    18841 tgaccgagat cgggatcccc gtcatgggcc acatcgggct caccccgcag ttcgtgaaca
    18901 ccatgggcgg gtaccgggtc cagggacgat ccgaacaggc ggcgaaccga ctgagcgagc
    18961 aggcggcatc cctggagaag gccggggtct tctcgatcgt cctggagggg gtgccggcgg
    19021 acgtgggccg cgtgatcacc cgcgacagct ccgtgcccac catcgggatc ggcgccggac
    19081 cccattgcga cgctcaggtg ctggtcgtcc acgatctgtt ggggatcaac gagcggcacc
    19141 cgaaattcgt gaaggcctac gcggatctcc gtggccagat cgccgccgcg gcgcgagcgt
    19201 tcgcggagga cgtggcggcg ggccggttcc ccggcgagga gcactcctac cactgatcct
    19261 ccctggttcc gggcggagat cgaggtcctg ctccgcgtct tcgtcgtgaa gcgtggcgcc
    19321 atcagcgagc gagggtcgtg ggcgagcctc ccgggagcag ccgcaccgga cccgacacgg
    19381 tgagggcggg gcagctgagg tcggtcctga gcggcgatcg cccgtccctg catgggatga
    19441 tcgaccggtg gaaccccgac gtgggatcgg gctgggggtg gtgctcgcgt gcctggcggc
    19501 caccctggga ctcggactgg cggtgaaggc tccctgcgcc gccggggact ggaccgacgg
    19561 acgtcagtac acgcgttact gctactcgga tatcgtgccg ctgctgtcca ccgaacagct
    19621 cacgggcaac cgcctcccct atctccagcc ttgcaccacc aacggtccgg gcgagtgtga
    19681 cgagtacccc gtcctctcga tgtacctgat gcgcctggcc gcctggatgt cgaacggctt
    19741 cgcaggcttc ttctacgcaa acgccctgct gctcaccatc gcggccgcgg tggccgtcgt
    19801 ctgcctgtac ctgttggttg gcagccgggc cgtgtacctg gcgctggcac ccaccctgtt
    19861 gctctacggc ttcatgaact gggatctgct cgcggtggcg ctggccacgg cgagcacgct
    19921 cgcttacctg aaccgccggg acgtctggtc cggagccctg ctggggctgg gggcggcggc
    19981 caaggtctac ccggcactgc tgctggtccc cttcgtcctg ggccggtttc gggaacgacg
    20041 gcccgacgcc ggcatccacc tcgcatgggc aggggcggga gcctggctcg cggtcaacct
    20101 gcccttcatc gtggcggccc cccggagctg gtgggagttc ttccggttca acggggccag
    20161 gccagcggac tgggatagcc tctggttcat cggctgccac aggatctcgg gaagcctggg
    20221 atgcaacgca ccggcgacca tcgggacggg caccgtcaac gtggcatccg ccgcggcctt
    20281 cgtggtcctt gcggtggccg tctgggtgct gcgacggcgg cgtgagccgg ggttcccccg
    20341 gtggaccttc gggttccccc tgctggtggt gttcctgctc accaacaagg tctattcgcc
    20401 ccagtacggc ctgtggctct tgccctggtt cgccctcgcc ctaccggacc tgcggttgtt
    20461 cgcgctgttc gaggcggccg atgtcgccgt gttcctcacc cgcttctcct ggttcggacg
    20521 cttctccggc acgggcgggt ggccgatcgg tgcgttcgag gtcgccgtgg tgatccgggc
    20581 ggcgatcctg atcgcttgcg tagtgacctg gatcctccgc cgcccggcac cggtccaggc
    20641 cgaggcacga ggggtggccg atcggagtcc ggcggatctg gcggcatgag tgcgacctcg
    20701 ctcaccctga gcgctcggac caggcggggg atccggtaca gcctcaccgt gttcgtcgcc
    20761 gtgaggatcg ggttgttcct ggtcgccctc gtggggctgg agctggtgcc gccgctgcgt
    20821 cccgtctcgg tccccggatg ggtggcgcat cccctcccgg accccggttg gcacaacctg
    20881 ttcacctcct gggagcggtt cgacgggctg tggttcctgc ggatcgcggc cggcggctac
    20941 cggtcggggg acggcagcgc ggcgttcttc cccctgtacc cgctggcgat ccgcgcggtg
    21001 tcctggctga tcggcggtca tcccttcgcc gcctcgctga tcgtgtccaa cgtctccttc
    21061 gccgccgccc tgtgcgtctt gtactcactg acggcggcag agcgctcgga ggagagggcc
    21121 cgccgagccg tgctgttcct cgccacgttc cccaccgcgc tcttcttctt cgcgccgtac
    21181 agcgaatccc tgttcctgct gctggccgtc acggcgttct gggctgcgcg ctcggatcgg
    21241 tgggggacag cggggatcgc ggcggcgttg gcggcgctca ccaggagcat cggcatcatc
    21301 ctggctcccg cgctcgtggt ggaggccttc ctgcgctggc gggagcgcgg gaggaacccg
    21361 gtcccgggga tcgcggcagg agccgccccc ggggtggcga ccctaggcta cctcgccttc
    21421 tgggggctcc ggagcggggc ctggctggct ccggtgcatc agcaggccaa ctgggaacgg
    21481 aggttctcgt ggccgtgggc cacgctggtg gaagccaccc gtttcgcctt ccggtacctg
    21541 ggcaacgcga acggcggcta ctgggcgatc gactggctca tcgtcgtccc ggcgcttgcc
    21601 ctgtcggtct acgccgtctt ccggctccgc gcctcctaca ccgtctacct gtggggcgga
    21661 ctgctggtgc cgctcttcta catcttcccc gggcgtccgc tcatgtcgat gccccggttc
    21721 ctgcttcccc tcttcccgct gttctgggcg gcatcggacc tgtccgagcg gtggcgcctg
    21781 ccccggggag cggtggccgc ggtgggagcc gggggtctgg ctctcctcac cgtgctgttc
    21841 gtcaactggt actacatctt ctgatggggg atgccgacat ataggatgag ccggcggaca
    21901 ccggtgccgg agcggtgaag gagcctgggg tgacgattcg ggtagccatc gcggacgacc
    21961 attccctggt ccgccagggg ctccgccgct acctcgacat ggccgagggc atcgaggtga
    22021 tcggcgaggc ctccaacggc gccgagctca tcgacctcgt cgacaaggag agccccgaca
    22081 tcgcgctggt ggacatccgg atgccggaga tggacgggct ggaggcggcg cgacagatcc
    22141 gggaccggtt ccagggggtc ggggtgatca tgctgaccgc ctacgacgac cgccagttcg
    22201 tggtggaggc cgtccggtcg ggcgctcgcg ggtacgtgct gaaggcccgg gatgccgagc
    22261 acctgatcca gacggtccgg ctcgtcgccg gcggcaacat ggtgatcgac ccgcagttgg
    22321 tcgtggctct cgccgaggag ctctcgcagg ccaaggagcg ggaccggagg gcagagacgc
    22381 tcaccgctcg ggagatcgag gtcctacagc tgctggcctt cggccacacg aaccgcgaca
    22441 tcgcggaacg tctgtacatc tcgcccgaca cggtcaagac gcatctcgag cacatcttcg
    22501 agaagctcgg agcctccgac cggaccgccg cggtcgccga agcgctccgg cgacggctca
    22561 tcgagtaggc gccgtgtccc gctccggcag gggttcagat gtcccccctc cggcgtaggt
    22621 ctcctgagct cggacgggcc cgtccgggtg atggagtctg agcggcgcac gaggcatcat
    22681 gaaggttcgg ggatgagcga tacgcacagc agctcgcgag gggcctccgc ctggccgatc
    22741 tccatcgcgc tcgtggcaac gatggggctc gtcgcggcgg cctacgcatt cgctcacgag
    22801 cggatcggat cccagccggc gctgtcgagc ctgatcgtgg cgatggcatg tgcgctggcc
    22861 atcctggcct cccttcccct ccgtcgcgct gccggcacgt ggcacagcct ccgtttcctc
    22921 ctcggagggg tggccgtggt cggtctggcg aacctgtggt tgctgtgggc cgaggccgag
    22981 aacatgcccc gatacgggcc gagcgcctac gacgccctgt tcctgctggc cgccgcgttc
    23041 ttctggatcc ccgcgcagct cgagtaccgg catcatttcc gcaaggacga tcggcgcgag
    23101 atcgccgccg acgtggccct catctcggcg gccctgggga cgatcctcta cctatggatc
    23161 cggccggatg gagccacgtt cacgagctcg ctgtccagcg ccgtcttcgc cctcatcgcc
    23221 gccaccgcgt tctcctcgta cggggccctg gccctgtggg tgccctcggt cggccacgcg
    23281 gggcagttcg cggcggtggg cgcgttctcc agcgccctga tggcgttcgc tacgccatgg
    23341 gtccgaggcg cgtaccggcc gggcacggtc caggtggacc tgcccctggc gctgggtgcg
    23401 ctcgggctgg ccgccctgct caccctggcc ccgcgctcga ctccgtccgg tccgatcacg
    23461 cgccccacac ggtacggacg cgccctgctc actacggtcg ccgtggcggc cgcctgcgcc
    23521 gccctgggcc tgatcgccgg cgccgagagc cgtggcgaga tcggcaacct gcacgcgacc
    23581 gggctgatct ggttgctggg ggcggcggtg gcgctccgca tcctggccaa ccagctccgc
    23641 agcacgcagg acgcccaagc ggcccgcgac gcgctggacc agaaggagat ggccctgctc
    23701 gagaccgaca aggccctggc ccgcctgcag cgagcgaacg agaccctgcg cgagtccgag
    23761 gaacggctcc ggctcgtctt cgaggccgcg gtcgacggga tcgtggagct cgatgagcac
    23821 gacgtgatcg tccgagccaa cgaggccttt tgtcagatgc tcgagcttcc ccagagcatg
    23881 gtcgaggggc agccctggag cgcgctcggg gcggtggtcc aggcggatga gtccttcggc
    23941 accctgcccc agaccggcca gggaacgctg atgcgcgagg ggcaagccat ctacctcgag
    24001 gcccggacct cggacgtgcc gggtgaccca ccgaggcggt tgctgctggt gcgggacgtc
    24061 acggccgccc gcgtcgccga ccagacgatc cggtcactgt tcaagttcct ccaggaccgc
    24121 gacgaggacc gcacccggat catgcgccgc accaatgcgg ccatcgaggg cgagcgcaac
    24181 cgcatcgccc gggacctgca cgacgggccg gtccaggggg tctccgccgc ttccttgtcg
    24241 ctggaggccg tcctgctgat gctgaaggcc ggggagaccg aggaaggcgt cgaggttctg
    24301 ggcaaggtcc gcaaggagct ggcggaggag gcggacaacc tccgccgact gatggccggc
    24361 ctgcggccac cgctgctgga ggagcgcggc ctggtccccg ccctgcgcga gaacctcgag
    24421 aagttcgggc acgacacggg gatccagacg gtcttccacg cgaacggggt caccgacgtc
    24481 ccccaggacc tggagacgct ggcctaccgg atcgtccagg aagccttgtc caacgccggg
    24541 aagcacgctc acgctgggcg catctccgtg ggggtggagg ccctgggcgg tcagctgcgg
    24601 atcgagatcg cggacgacgg gaaaggcttc gactcggcca aggcgaggga gttcctgcgc
    24661 ctgggtcggg tggggctcgc ctccatgcgg gagcgggtgg agctggccaa cggcacgttc
    24721 atggtccact ccacgcccgg gaagggaacg acggtcctcg ccaccctgcc cctggagtcg
    24781 gttcccgggc cacgggagtt cgcggtcaac gaggccttct agctccctcg ccccccaccg
    24841 gcgtggcggg tgggggcggg ctcggtgtcc cggagggcgt gcccgacggg ctcgggctcg
    24901 ggctggggct ggttgagggg ctggggctgg ggctggggct ggtcgagggg ctggggctgg
    24961 gcgacgggct cggcttcg
//
LOCUS       DASQQQ010000104         2893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_88996, whole genome shotgun sequence.
ACCESSION   DASQQQ010000104 DASQQQ010000000
VERSION     DASQQQ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88996"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VE646_04330"
     CDS             complement(<1..745)
                     /locus_tag="VE646_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HZD79252.1"
                     /translation="MGVQSRNGGAWLIVGLALAVGSQAKAQGQKEIGSVREALDVSAR
                     LAGSSGPASVNWIESGERFSYTTRGARGTAEVRVYNPETLADEALFDARRLTLPGTAE
                     PIAYRSFEFGQDSRHLVFQTHFRRIYRNSGISDFYLYTLGEQGVRPLVKNARTADLSP
                     SGERLGFERGGNLYVIDLEDGSEQQLTGVAGDSIFDGVFDWVYEEEFGFAQAWKWSPD
                     SRRIAYWQTDERGVPVIQLTDWSEQHPDWF"
     gene            1043..2101
                     /locus_tag="VE646_04335"
     CDS             1043..2101
                     /locus_tag="VE646_04335"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HZD79253.1"
                     /translation="MAPDPALMLLAFAAVAAITLVLLWPARRALAGLRRVRREAERVR
                     LEDALKHMHECERAGRACTLENLAGRLETSRGAASDLLTRLTERGLAVVGPSGVELTG
                     PGRAYALRIVRTHRLWERYLADRTGVPAVDWHEEAERVEHELTQEEAEDLAARLGHPR
                     YDPHGDPIPTAGGEIPAPVGVPLPRVVPGRHVVVRHLEDEPREVFERLVGLGLAPGVP
                     LEVLDGEGEGEGAARGAVRIRVQGVERSVRAADAGNVTVEEVAEDVGSEGRSRTLADA
                     EPGQEVRVTSLSPACQGPQRRRMLDLGVVPGTRITPELVSTSGDPVAYRIRGALIALR
                     RHQASWIEVDAEPVERAG"
     gene            2103..>2893
                     /locus_tag="VE646_04340"
     CDS             2103..>2893
                     /locus_tag="VE646_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HZD79254.1"
                     /translation="MATRLKPGAPGGAAQHRRQTLVRLGLSPDRWDYLVALAGNPNVG
                     KTTVFNGLTGLRQHTGNWPGKTVARAEGAFAHNGKRVKVVDLPGTYSLQAGSSDEEVA
                     RDFIVFGRPDVTVVVVDATRLERNLNLVLQILEITDRVVVCLNLVDEARRHGVSIDAK
                     RLERELGVPVVPAVARQGEGIEELLAAVEGVATGRIPTTPYRVQRHAPEVEGAVAELV
                     PAIERAYPGVPNPRWVALRLLNADAAVEEAVRDGELGLLGHGGPET"
BASE COUNT          407 a          995 c         1091 g          400 t
ORIGIN      
        1 ggaaccagtc cgggtgctgc tccgaccagt cggtgagctg gatcaccgga accccccgct
       61 cgtccgtctg ccagtacgcg atccgtcggc tgtccgggct ccacttccac gcctgcgcga
      121 acccgaactc ctcctcgtag acccaatcga acaccccgtc gaagatgctg tcgccggcca
      181 ctcccgtgag ttgctgctcc gacccgtcct ccaggtcgat cacgtacagg ttcccgcccc
      241 gctcgaaccc cagccgctcc ccgctcggag atagatccgc cgtccgcgcg ttcttcacca
      301 gcggcctcac cccctgctcc cccagcgtgt acaggtagaa atccgagatc ccggagttcc
      361 ggtagatccg acggaaatgc gtctggaaca ccaggtgcct cgagtcctgc ccgaactcaa
      421 acgatcggta cgcgatcggc tccgccgtcc ccgggagcgt cagccgccgc gcatcgaaca
      481 gcgcctcgtc cgccagcgtc tccgggttgt agacccggac ctccgccgtc ccccgggcgc
      541 cccgggtcgt gtacgagaac cgctcgcccg actcgatcca gttcacgctc gccggaccgg
      601 acgatcccgc caggcgcgcc gaaacgtcca gtgcctcccg cacgctcccg atctccttct
      661 gcccctgcgc cttcgcctgc gatcccaccg ccagcgccag cccgacgatc agccacgccc
      721 ccccgttcct gctctgcaca cccatgatct cgccctctct attcggccac ggcccctgac
      781 ggaccgccgt cacgtcccgc ttcacagccc ggaaaatacc gggcgcctcc aacccgcgca
      841 cgcgcccgcg cccgccgctc acctggtccg cccggctcga tcactggccc gaaacgtgac
      901 cgcgtgcggg agcgtgagcg agggcgccat cgtcccccgt cgaccgtact cctgcgtcgc
      961 aaggaaaggt ccgatcggcc gagggtcttg accgctgcgc cccctcagac aaaatttagc
     1021 cgagactaaa aacggaggga ccgtggctcc tgaccccgca ctcatgctcc tcgccttcgc
     1081 cgccgtcgcg gcgatcaccc tcgttctgct ctggcccgca cgccgcgccc tggccggcct
     1141 ccgtcgggtc cggcgggaag cggagcgggt gcgcctcgag gacgcgctca agcacatgca
     1201 cgaatgcgag cgggcgggtc gcgcctgcac gctggagaac ctggcggggc gactggagac
     1261 gtcgcgggga gcggcgtcgg acctgctgac ccggctgacg gagcgcggac tggcggtggt
     1321 cggcccgtcg ggggtggagc tgacgggtcc cgggcgagcg tacgcgctgc ggatcgtgcg
     1381 gacgcatcgg ctgtgggagc ggtacctggc ggaccggacg ggggtgccgg cggtggactg
     1441 gcacgaggaa gcggagcggg tggagcacga gctgacacag gaggaggcgg aggacctcgc
     1501 ggcgcgtctg ggccacccgc gttacgatcc gcacggagac ccgatcccca cggcgggcgg
     1561 ggagatcccg gcgccggtgg gcgtgccgct cccgcgggtg gtgccgggcc gtcacgtcgt
     1621 ggtccggcac ctggaggacg agccgcggga ggtgttcgag cggctggtcg gcctggggct
     1681 ggcgccgggt gtgccgctgg aggtgctgga cggggagggg gagggggagg gggcggcccg
     1741 gggcgcggtg cggatccggg tccagggcgt ggagcggagc gtccgggcgg cggatgcggg
     1801 caacgtgacg gtggaggagg tggcggagga tgtcgggtcg gagggtcggt cacggacgct
     1861 ggcggacgcg gagccgggcc aggaggtgcg cgtgacgagc ctgtcgccgg cgtgtcaggg
     1921 tccgcagcgt cgtcgcatgc tggacctggg ggtggtgccg gggacgcgaa tcacgccgga
     1981 gctggtgagc acgtcgggcg acccggtggc gtaccggatc cggggtgcgc tgatcgcgct
     2041 gcggcgccac caggcgtcgt ggatcgaggt cgacgcggag ccggtggagc gggcgggctg
     2101 atatggcgac gcgactgaag ccgggggcgc ctgggggcgc cgcacagcac cgtcggcaga
     2161 ccctcgtgcg cctgggtctg agtcccgacc gctgggacta cctggtggcg ctcgcgggga
     2221 acccgaacgt cggcaagacg acggtgttca acggcctcac ggggctccgg caacacaccg
     2281 ggaactggcc gggcaagacc gtggcgcggg cggagggcgc gttcgcgcac aacggcaagc
     2341 gtgtgaaggt ggtggatctg cccggcacct actcgctcca ggccgggagc agcgacgagg
     2401 aggtggcgcg cgacttcatc gtcttcggcc gcccggacgt gacggtggtc gtggtggacg
     2461 cgacgcggct cgagcggaac ctgaacctgg tgctgcagat cctggagatc acggaccggg
     2521 tggtggtctg cttgaacctg gtggacgagg cgcggcgcca cggggtgtcg atcgacgcga
     2581 agcggctgga gcgcgagctc ggggtgcccg tggtcccggc ggtggcccgc cagggggagg
     2641 gaatcgagga gctgctcgcg gcggtagagg gcgtcgcgac cgggcggatc ccgaccactc
     2701 cctaccgggt gcagcgccat gcgccggagg tggagggtgc ggtggcggag ctggtgcccg
     2761 caatcgagcg ggcgtatccc ggcgtcccga atccccgctg ggtcgcgctc cggctcctga
     2821 acgcggatgc ggccgtggag gaggcggtgc gggatggtga gctcgggctc ctcggccacg
     2881 gcggtccgga aac
//
LOCUS       DASQQQ010000105        15643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_34803, whole genome shotgun sequence.
ACCESSION   DASQQQ010000105 DASQQQ010000000
VERSION     DASQQQ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15643
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34803"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            427..1899
                     /locus_tag="VE646_04345"
     CDS             427..1899
                     /locus_tag="VE646_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZD79255.1"
                     /translation="MRLEMRVTIWLTALLVAATALHLVGMATGAYSFVFMTVPLVLGI
                     VLSVWAVRRTVLKPLAQICTAARDLGAGDLSVRLPDFDSAELEEVSRTFNETARMLED
                     RAADLAEAITRLRSDLEGLEEIQGVLASGAGLPQVLSRVARTLGTALGANGAAIWRVG
                     SDVPEVVEGGPLPPVEGLPGAAGGVPVTSHGALADVPEDAPLSWALAPARRDGRVVGV
                     VGAAWDPSRPIDRMQRDLLVALAGLTAVAIENVHLLDRVTEKEQSLEGLLRKTLSTQE
                     EERRRISRELHDETSQVLSALMMNVDLLERHIEDREPAVDGSLARVEAVKGLAEEAAR
                     NLDKMMLELRPALLDELGLMPALRWHLTQASDLWGVPIRFEGRSVGRLADVVEVAAFR
                     IVQESVSNCVRHATASVIDVRVCADDRMLHVEVDDDGVGFDTGPLPSLSRTGRAVGLV
                     GMRERAELVGGSFHVTSSPGHGTRVRAELPLLPPGWRNDE"
     gene            1892..2557
                     /locus_tag="VE646_04350"
     CDS             1892..2557
                     /locus_tag="VE646_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD79256.1"
                     /translation="MSDRIRVLVVDDHEVVRQGIKMVLDSDADLEVVGEAGSGEEAID
                     AVEELDPSVVVMDVGLPGLSGFEATRLIKRAHPDIQVLALTVHDSEAYVFQMLRAGAT
                     GYVVKRAPAADVIQAVKHAFAGEAVLHPSAARLLIRDYLSRVAQGEEVSYDTLSQRER
                     EILRLIAEGKTNKEIAVLLDVSVKTVQAHRANLMHKLGMHDRTELVKYAIRRGIIGLD
                     ETA"
     gene            2633..2779
                     /locus_tag="VE646_04355"
     CDS             2633..2779
                     /locus_tag="VE646_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79257.1"
                     /translation="MAHAKDKGGEKHSKRAAQKSLKERRAQKRAKRESSQHPSTVHIT
                     TTSQ"
     gene            2782..3852
                     /locus_tag="VE646_04360"
     CDS             2782..3852
                     /locus_tag="VE646_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HZD79258.1"
                     /translation="MFEELLEAWDGEQVAVRYDRQLRTWMFVCVHSTRLGPGAGGTRM
                     KVYPSPHDGLRDGLRLSAAMTSKNTMAGLPLGGGKAVLAVPEVPEGDRRREVLLAYAD
                     LVESLHGTYWTACDMNTTPQDMDAIAERCGSVFGRSEAGGGSGSSAPATAIGVFHGIQ
                     ACAREVFGTPDLGRRTVLVQGVGAVGRVLAERLHEAGAELILADADPARAEHAAGRLG
                     ARTVPAESAIDTPCDVFSPCATGAVLSEETIPRLRCRIVAGAANNQLARSEDAERFRP
                     LGILYAPDYVINAGGIIHLASLELLGEDLAARDERVLAIGDTLTELFRRAEEQGLSTT
                     AAADEVVAARLAAGGGTRLAPA"
     gene            3885..4244
                     /locus_tag="VE646_04365"
     CDS             3885..4244
                     /locus_tag="VE646_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HZD79259.1"
                     /translation="MARDLASMQCVPCRGGIPPLTHEQIEPYLADLGGDWQVEEDHHL
                     SKTYRFPNFREALDFTNRVGQLAEEVGHHPDIALSWGRVGLTVWTHKIGGLHEADFVF
                     AAKADRAFEGWTAAHQE"
     gene            complement(4261..5538)
                     /gene="hemL"
                     /locus_tag="VE646_04370"
     CDS             complement(4261..5538)
                     /gene="hemL"
                     /locus_tag="VE646_04370"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HZD79260.1"
                     /translation="MVTGSEELFERARRVIPGGVNSPVRAFGAVGGTPPFIVRAEGAF
                     IEDADGNRYVDLVQSWGASLFGHACPELVEAATQAARRGTSYGAPTEAEVELAESIVQ
                     ALPSVEMVRLVSSGTEACMSAIRLARAATGRSKILKFDGCYHGHSDPLLAKGAGSGVA
                     TFGLPASPGVTEGAARDTLQVPYNDLEAVRALFGRNGPRIACLIVEPVAANMGVVPPE
                     PGFLQGLRELCSEHGALLLFDEVITGFRIAYGGAQSLYGVEPDLTVLGKVMGGGFPCA
                     GFGGRARVMEHLAPQGPVYQAGTLSGNPVAVAAGLAALRLARERDPYPALVRTARELT
                     SGLAQGFGRTGMAATINRAGSMFSVSFGPGPVRDYAQARAADQERYGRFFHHMLERGV
                     YLPPSGFELWTLTTTHGPEEVGRVMEAAASFEG"
     gene            5574..6233
                     /locus_tag="VE646_04375"
     CDS             5574..6233
                     /locus_tag="VE646_04375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZD79261.1"
                     /translation="MGDDRPILDAPGGPMACFFAAPEGRPPAPGVVVVQEWWGLNGQI
                     EGVARRLAGEGFAALAPDLYRGKQADEPHEARKLSMQLDRGRALQDLRAAVGWLLEHG
                     AGAVGAIGFCMGGSITFELACIEERLGAAVPFYGLSDLRERTITCPVQAHFGTADRFD
                     PELLDDVAWRLGDLGNGSELHRYEGAPHAFMNETRRSYRPQAAAVAWERAISFLRANL
                     S"
     gene            complement(6243..6767)
                     /gene="msrA"
                     /locus_tag="VE646_04380"
     CDS             complement(6243..6767)
                     /gene="msrA"
                     /locus_tag="VE646_04380"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013554192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HZD79262.1"
                     /translation="MAIQTATLGAGCFWGVEWVFRQVPGVIDVVSGYSGGHTENPTYR
                     EVCTHTTGHAEVVQITFDDQRVSYGQLLEVFWAMHDATQMNRQGPDVGSQYRSIILAH
                     SEEQRATAEASKASAQARISRPVATEIVPFEDFYPAEDYHQRYYDKTGHEPYCHVLPV
                     RTLQAMGLVPAGRR"
     gene            complement(6953..7939)
                     /gene="hemB"
                     /locus_tag="VE646_04385"
     CDS             complement(6953..7939)
                     /gene="hemB"
                     /locus_tag="VE646_04385"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012688931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HZD79263.1"
                     /translation="MATFPQHRPRRLRRTPALRSLIRETELAPRHLIAPLFVKEGIAE
                     PRPIVSMPHQFQHTLESLRKEAAEVAARGVTAFMLFGVPERKDPEGSEAWNPDGIAQK
                     GLRLLREELGDDHVVIADLCLDEYTDHGHCGVLDHEGNVDNDATLERYRKIAVAQAEA
                     GADLLGPSGMMDGQVAAIREVLDKAGLQRVGIVAYAAKFASSFYGPFREAAECAPRFG
                     DRTGYQMDPPNSDEAIREITTDIEEGADIVMVKPALPYLDVIRRAKEETRFPLAAYNV
                     SGEYSMVKAAAQNGWLDGRRATLEVLTSIRRAGADLILTYHAKEAADWLTGR"
     gene            8013..8732
                     /locus_tag="VE646_04390"
     CDS             8013..8732
                     /locus_tag="VE646_04390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HZD79264.1"
                     /translation="MQPAYDAALLREGEAFAAAAQMGLDLPVPSCPGWSVAELTWHLG
                     RVHRHWEERVRLRLQEQAGLTEVERPPHRELVPWFRDGLERLSQALRGADPGERIWTW
                     ARQQDVGFVRRRMAQETVVHRVDAQLAAGSFEPIEPVLAADGVDEFLDVMLGEDEERL
                     REGTETIHLHATDIEAEWLVEVADGRLTNRHEHGRGEAAARASASGLLLLLWRRIAPD
                     RVEVLGDRSVLRRFLARADLG"
     gene            complement(8729..9538)
                     /locus_tag="VE646_04395"
     CDS             complement(8729..9538)
                     /locus_tag="VE646_04395"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZD79265.1"
                     /translation="MTGPLDGAVVLVTRPRAQADELIRALEARGATAVAAPAVEILPA
                     RSAALTAALRELSMGRFDWLTLTSRNTVDMLEARLLPEDVRARVAVIGEGTAEAFRAW
                     TGRDPDLMPRTFTTAALSRTFPRGTGRVLCARADIAPEGLEDALAAKGWAPVRVHAYR
                     TRTPTSLPRAARGVLRDGSVDAITFTSASTVRGFVGTVGALRGNPKVICIGPVTAREA
                     RARGFRVSGVADPHTIEGLVDAVERALRARTARGTCAGGPAAAPSGMSEGP"
     gene            complement(9535..10428)
                     /gene="hemC"
                     /locus_tag="VE646_04400"
     CDS             complement(9535..10428)
                     /gene="hemC"
                     /locus_tag="VE646_04400"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HZD79266.1"
                     /translation="MKLRIGTRRSRLAMAQAEAVARALRNAGAEPEVVPMQTAGDRGG
                     AGGASPAGLKGLWVSDIVRALREGEVDLAVHSAKDLPAEDEDGVALAAVPERASPLDV
                     LITPDGHLPQGSGLGTSSLRRRSQVLRWRPDLRVVELRGNVDTRLAKLERGEVDGLVL
                     AAAGLLRMGIVPEHAAPMSAAEMVPAPGQGCLGIQARSADPGVLEVLSRLDHRDSRTA
                     FEAERRLVRLLGGGCALPLGAYARVQDQRVRLVAVVLTSDGDRAARVDVEATSPEVAA
                     DVAAQRLRSEGAEQILAEARA"
     gene            complement(10425..11696)
                     /locus_tag="VE646_04405"
     CDS             complement(10425..11696)
                     /locus_tag="VE646_04405"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:NF000744.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HZD79267.1"
                     /translation="MAILALGVSHRRATVELLEQLAFTDDDFTKAYRRAQDDDALDEA
                     VILSTCNRVEIYASVPSYHAGFLSLKRLLAESRGAHPDLLTEPLYSHYDEAAAQHLFA
                     VAAGLDSMVLGEPQILAQVREAHRRAAAEGAAGHALTALFHAAARAGRRVRSETGVGA
                     APDAFVEAGAHLAEERLAGLAGKSAAVVGAGQMAALAVKHLRARGVGPVRVLNRSLER
                     ARALAERTSAEPDDLAGLPGALAGADLLVSATGAAGAVIHHDTVAAALNGRTRPLFVL
                     DLAVPRDVEPSVAELDRVTLVDIEGLRTALATPAAEASEDIRRAEAVVAEELHRFAIR
                     QRSERLAPVIRALRERGDDVLTAETRRFRAELSRLTPDERDAVEAMARGIVAKLLHEP
                     IVRLKELSTPGTEDLHARMLADLFGLDPRSE"
     gene            11818..12930
                     /locus_tag="VE646_04410"
     CDS             11818..12930
                     /locus_tag="VE646_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HZD79268.1"
                     /translation="MERGRARDLGVVVGMLPTGARNAITDVAGVRVGHATLIRDPEPG
                     GRGAVRTGVTAVFPHQGLPWVERVYAGTHILNGYGELIGINQIAEWGLLHSPVVMTSS
                     LAIGRAYDATVRWRTERHGVTTHDGGDMPIVTECDDSFLNDVTTFPLRDQDVFAALDS
                     AESGDVAEGCVGAGTGMQCFDFKGGIGTASRVLPAGLGGYTVGALVLTNFGAREELIV
                     DGVPVGREISDLMPEEHGEGSCICLVATDAPMLPHQVRRLAVRAGLGLARTGSTARNT
                     SGEQMLAFSTANRLPMETPAGALEVRALLDGAAESSPPILSGLFSAVIEAVEEGVLNA
                     LFTATTTTGRDGNTLHALPLDRTLGLLERHGRLRSA"
     gene            13034..13558
                     /locus_tag="VE646_04415"
     CDS             13034..13558
                     /locus_tag="VE646_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZD79269.1"
                     /translation="MDVNGGFRRMLQERFGRVREAMTSSVVELEPSLLAAEAVRLLSE
                     AGVTGGPVVEDGRVVGIVTLSDLIGREHHAAAHVTGPFLRSDRQLGNLKVTDVMTREV
                     VTACEDWPLVRAIVTMDDAGVNRLPVLDAFDHPVGILARDDVVRAVAAALRWAERAEE
                     HGAQVPDWTLLEAD"
     gene            13609..13697
                     /locus_tag="VE646_04420"
     tRNA            13609..13697
                     /locus_tag="VE646_04420"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13643..13645,aa:Ser,seq:gct)
     gene            13854..14006
                     /locus_tag="VE646_04425"
     CDS             13854..14006
                     /locus_tag="VE646_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79270.1"
                     /translation="MTYHDPHEPVREPDSGGLVGFAAIKYTAIVVIVLAILAFIAFYL
                     IPAVTS"
     gene            14057..14191
                     /locus_tag="VE646_04430"
     CDS             14057..14191
                     /locus_tag="VE646_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPGG-motif small membrane protein"
                     /protein_id="HZD79271.1"
                     /translation="MLWIIAAVFVIWGIVTLIRGGVLAGILLIVVGLLIGPGGVSIFT
                     "
     gene            14239..15537
                     /locus_tag="VE646_04435"
     CDS             14239..15537
                     /locus_tag="VE646_04435"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD79272.1"
                     /translation="MERRAGFVDRIGALRPLQIRDFRLLWTGLTVSMIGDGVYVVAVA
                     LQVLDLSNSPTAIAGVGIAWTAPQVVLMLGSGALADRIDRRRLMVSGDLIRLVAIGTL
                     GTLSIADRLTLPMLFALVVVYGVGQAVFAPAFSSIIPTIVPDDLLVQANSLGQVVRPA
                     AMMLIGPLVGGILAGSLGPGWAFVMDGASFGWSAVMIVLMRVRPLPRTEEPTRVWADV
                     KQGLRYVRSETWIWAALVAATVSLLCTWGPWEALVPFVVRNQLAPSGGAGVALGLVFG
                     AGGLGAVGAALVLGQREGLPRKPVTVLYLAWALGMVMTAGFGMVVTVWQGMIVAFVSE
                     GSIAVLVVIWYTLLQRLVPRDLLGRVSSLDWLISIAGVPLSYAIVGPMAGWVGADATL
                     IGAGLLGGAITLAFMFIPGARSPERDGSLEPRHEGAAVRS"
BASE COUNT         2127 a         5411 c         5833 g         2272 t
ORIGIN      
        1 ccccgccgcg tgctcaagac cccggctcgg gccagagacc ggacgatctt gctcaggaat
       61 gcgggggaga tctcttcctg ggcggcgacc tgcgtcacgc tcaggtggcc ctggcgagcg
      121 acctcgagga cgcagcggac cgcgtagtcc gcctccctcg tcatgatcat cgccgtccac
      181 ctccggtcct cgaccggccg cccgggtcgc cgcccgatgc ctttgcgcaa gggccggtct
      241 ccggaccttt ctggtacgga tggtccctgt tcgagcgatc ggcaccacgg gtcgagggtc
      301 ctgcggcccg ggggtcgagc ggcgaaccgg agaccctagg tccgagaccg tacggccccg
      361 ccgaccaggg acttccgccc ccaccacgtc cctcccggac gaagtgaagt ctcttcggga
      421 ggttccgtgc gactcgagat gcgcgtcacc atctggttga cggccttgct ggtggccgcc
      481 accgcgctgc acctggtggg gatggcaacc ggcgcgtact cgttcgtgtt catgaccgtt
      541 ccactggtgc tgggcatcgt gctgtccgtg tgggccgtcc gccgcaccgt cctgaaaccg
      601 ctggcccaga tctgcacggc ggcccgcgat ctgggggcgg gcgacctgtc cgtgcgtctg
      661 ccggacttcg acagcgccga gctcgaggag gtctccagga cgttcaacga gaccgcccgc
      721 atgctcgagg accgggcggc cgatctggcc gaggccatca cccggctccg gtccgatctg
      781 gaagggctcg aggagatcca gggcgtgttg gcctcgggcg ccggtctgcc ccaggtcctg
      841 tcccgcgtcg cgcggaccct cggaaccgcg ctgggggcca acggtgcggc gatctggcgc
      901 gtcggctcgg acgtccccga ggtcgtggag ggcggtcccc tcccgccggt ggagggcctc
      961 cccggtgcgg ccgggggcgt cccggtcacc tcccacggtg ccctggccga cgtccccgaa
     1021 gacgcgccgc tctcttgggc gcttgctccg gcccgacggg acgggcgggt cgtgggcgtg
     1081 gtgggggcgg cgtgggatcc ctcgcgtccg atcgatcgca tgcagcgcga cctgttggtg
     1141 gccctagccg ggctcaccgc cgtggccatc gagaacgtcc atctcctgga ccgggtcacc
     1201 gagaaggaac agagcctgga ggggctgcta cgcaagaccc tcagcacgca ggaggaggaa
     1261 cgccgccgga tctcccggga gctgcacgac gagaccagcc aggtgctctc ggcgctgatg
     1321 atgaacgtcg acctgctgga gcgccacatc gaggaccggg agccggccgt ggacgggtcg
     1381 ctggcacggg tggaggcggt gaagggtttg gccgaggagg ccgccaggaa cctggacaag
     1441 atgatgctcg agctccgccc ggcgctgctg gacgagctcg ggctgatgcc ggcgctgcgg
     1501 tggcacctca cccaggcgag cgatctgtgg ggggtgccga tccggttcga gggacggagc
     1561 gtcggacgcc tcgccgatgt cgtggaggtg gccgccttcc gcatcgtcca ggagtcggtg
     1621 agcaactgcg tgcggcacgc cacggcgtcc gtgatcgacg tgcgggtctg tgccgacgac
     1681 cggatgctgc acgtggaggt ggacgacgac ggcgtgggct tcgacacggg gccgttgccg
     1741 tcactgtcac ggaccggccg ggcggtcggt ctggtgggga tgcgggagcg ggccgagctc
     1801 gtcggcggct cgttccacgt gacgtcctcg ccgggccatg ggaccagggt gagggccgag
     1861 ctgccgctcc tgccaccggg atggaggaac gatgagtgac cggatcaggg tgctggtggt
     1921 cgacgaccac gaggtcgtgc gccaggggat caagatggtc ctggattccg acgccgacct
     1981 ggaggtggtg ggcgaggcgg gctcgggcga agaggccatc gacgcggtgg aggagctcga
     2041 tccgagcgtg gtggtgatgg acgtgggctt gcccggcctg tccgggttcg aggccacacg
     2101 gctgatcaag cgcgcccatc ccgatatcca ggtgctggcc ctcacggtgc acgacagcga
     2161 ggcgtacgtg ttccagatgc tccgggccgg ggctacggga tacgtcgtca agcgggcgcc
     2221 ggcggcggat gtgatccagg cggtgaagca cgcgttcgcc ggggaggccg tcctgcatcc
     2281 cagcgccgct cgcctgctga tccgcgacta cctctcccgg gtggcgcaag gggaggaggt
     2341 ctcctacgac acgctgtcgc agcgcgagcg ggagatcctg cggctgatcg ccgagggcaa
     2401 gaccaacaag gagatcgccg tcctgctcga cgtctcggtg aagacggtgc aggcgcatcg
     2461 ggccaacctg atgcacaagc tggggatgca cgatcggacc gagctggtga agtacgcgat
     2521 ccgccggggc atcatcgggc tcgacgagac cgcctgaggc ggagcggacg cgcagacgca
     2581 cccggtcttc ccggcgggcc ataatggatg ggtagccgaa ggaggtccca ccgtggcgca
     2641 cgccaaggac aagggcggcg agaagcacag caagcgggcg gcgcagaaga gcctgaagga
     2701 acggcgggcg cagaagcgcg ccaagcggga gtcctcgcag cacccctcga ccgtgcacat
     2761 cacgacgacg tcccagtagc cgtgttcgag gagctgctcg aggcctggga cggtgagcag
     2821 gtcgccgtgc gctacgaccg ccagctccgg acctggatgt tcgtctgcgt ccactcgacg
     2881 cggctcggtc cgggagcggg cggcacccgg atgaaggtct acccgagccc gcacgacgga
     2941 ctccgggatg ggctgcgcct ctcggccgcc atgaccagca agaacacgat ggccggcctt
     3001 ccgctagggg gcggcaaggc ggtgctggcg gtgcccgagg tgcccgaggg cgatcgccga
     3061 cgggaggtct tgctcgccta cgccgacctg gtcgagtccc tgcacggcac ctactggacg
     3121 gcctgcgaca tgaacaccac gccacaggac atggacgcga tcgccgagcg gtgcggctcc
     3181 gtattcgggc gctcggaggc gggaggggga tcgggcagct cggccccggc cacggccatc
     3241 ggcgtcttcc acgggatcca ggcgtgcgcg cgggaggtgt tcgggacccc agacctgggc
     3301 cgtcgaacgg tcctggtcca gggcgtgggt gcggtgggcc gcgtgctggc ggaacgcctg
     3361 cacgaagcgg gagccgagct gatcctggcg gacgccgacc ccgcccgtgc ggagcatgcc
     3421 gccgggcggc tgggcgccag gaccgttccg gcggagtcgg ccatcgacac cccctgcgat
     3481 gtgttctccc cgtgcgccac gggcgccgtg ctctctgagg agacgatccc ccgcctgcgc
     3541 tgccgcatcg tggccggggc cgcgaacaac cagctggccc ggtcggagga tgccgagcgc
     3601 tttcgcccgc tcggcatcct gtacgcgccc gactacgtga tcaacgcggg cgggatcatc
     3661 cacctggctt cgctcgaact gctgggggag gacctggccg cgcgggatga gcgggtgctc
     3721 gcgatcgggg acacgctgac tgagctcttc cggcgggcgg aggagcaggg cctgtccacg
     3781 accgccgcgg ccgacgaggt ggtggccgct cggttggcgg ccgggggcgg cacgcgtctg
     3841 gcaccggctt gacgcaacgg cggcctgccc ccaccatcgt ccccatggcg agggatctgg
     3901 cgtcgatgca gtgcgtgccg tgccgaggag ggatcccccc gctcacgcac gagcagatcg
     3961 agccttatct ggccgacctt ggcggcgatt ggcaggtgga ggaggaccac cacctgtcga
     4021 agacctaccg gttccccaac ttccgggagg cactggattt cacgaaccgg gtgggccagc
     4081 tggccgagga ggtcggccac catccggaca tcgcgctgtc ctggggccgg gtcggcctga
     4141 ccgtctggac ccacaagatc ggagggttgc acgaggcgga cttcgtcttc gcggcgaagg
     4201 ccgaccgcgc cttcgaaggg tggacggccg cccaccaaga gtgactctgg tcgggacgcc
     4261 tcagccctcg aagctcgccg ccgcctccat cacccgtcct acctcctcgg gtccgtgcgt
     4321 ggtggtcagc gtccacagct cgaagccgga gggcggcagg tagacgccgc gctccagcat
     4381 gtggtggaag aagcggccgt accgctcttg gtctgccgcc cgcgcctgcg cgtagtcacg
     4441 gaccgggccg gggccgaacg agacgctgaa catcgagccg gcccggttga tggtggcggc
     4501 catgccggtc cgcccgaagc cctgggccag gccggacgtg agctcccggg cggtgcggac
     4561 cagggccggg taggggtcgc gctcgcgcgc cagccggagc gccgcgagcc cggccgccac
     4621 cgctaccggg ttccccgaca gcgtgccggc ctggtacacg ggtccctgcg gcgcgaggtg
     4681 ctccatcacg cgagctcgtc ccccgaagcc ggcgcagggg aagccgccgc ccatcacctt
     4741 gcccagcacg gtgagatcgg gctccacccc atagagggac tgcgcgccgc cgtaggcgat
     4801 ccggaacccg gtgatgacct cgtcgaagag cagcagcgct ccgtgctcgg agcagagctc
     4861 gcgaagaccc tgcaggaaac cgggctcggg cggcacgaca cccatgttgg cggccaccgg
     4921 ctccacgatc aggcaagcga tccgggggcc gttccgcccg aacagggcgc ggaccgcctc
     4981 gaggtcgttg taggggacct gcagggtgtc tctggccgcg ccctcggtga cgccgggcga
     5041 ggccggcagg ccgaacgtgg ccacgcccga cccggccccc ttggccagca aggggtccga
     5101 gtggccgtgg tagcagccgt cgaacttgag gatcttcgat cggcccgtgg cggccctggc
     5161 caggcggatg gcgctcatgc aggcctcggt ccccgaggag accagccgga ccatctccac
     5221 cgaaggcagg gcctgcacga tcgactcggc cagctcgacc tcggcctccg tgggggcgcc
     5281 gtaggaggtc ccccgccggg ccgcctgcgt ggcggcctcc accagctcgg ggcacgcgtg
     5341 gccgaacagc gatgcccccc aggactggac caggtccacg taacggttgc cgtcggcgtc
     5401 ctcgatgaac gcgccctccg ctctgacgat gaagggcggc gtgccgccca ccgccccgaa
     5461 ggcccggacc ggggagttca cccccccggg gatcacccgg cgggcacgct cgaacagctc
     5521 ctcggacccg gtcaccatca cccagtgtag gcgggtggga tacgctcgca ccggtggggg
     5581 acgaccgccc gatcctcgac gcgcccggcg ggccgatggc ctgcttcttc gccgctcccg
     5641 agggccgccc gcccgccccc ggcgtcgtgg tggtgcagga gtggtggggg ctcaacgggc
     5701 agatcgaggg cgtggcgcgc cggctggcgg gggaggggtt cgcggccctc gcccccgacc
     5761 tgtaccgggg caagcaggcc gacgagccgc acgaggcccg caaactgtcg atgcagctgg
     5821 accgggggcg ggccctgcag gacctgcgcg ccgcggtggg ctggctcctc gagcacggag
     5881 ctggggcggt gggcgcgatc ggcttctgca tgggcgggtc catcaccttc gagctcgcgt
     5941 gcatcgagga acggctcggc gccgcggtcc ctttctacgg gctgtccgac ctgcgggaac
     6001 ggacgatcac gtgtccggtg caggcgcatt tcggcaccgc ggatcggttc gatcccgagc
     6061 tcctggacga cgtggcctgg cgcctgggcg acctgggcaa cggcagcgag ctccaccggt
     6121 acgagggcgc gccgcacgcg ttcatgaacg agacgcgtcg ttcgtaccgg ccccaggccg
     6181 ccgccgtcgc ctgggagcgg gcgatctcgt tcctgcgggc gaacctgagc tgagggaggg
     6241 cctcagcgcc ggccggctgg caccagcccc atggcctgca gggtccgcac cgggaggacg
     6301 tggcagtagg gctcgtggcc ggtcttgtcg taatagcgct ggtggtagtc ctcggcggga
     6361 tagaagtcct cgaaggggac gatctcggtg gcgacgggcc gggagatgcg cgcctgcgcg
     6421 ctggccttgg acgcttcggc cgtagctcgc tgctcctcgg aatgggccag gatgatgctc
     6481 cggtactggc tgcccacgtc gggaccctgg cggttcatct gggtggcgtc gtgcatcgcc
     6541 cagaacacct cgagcagctg cccgtacgac acgcgctggt catcgaaggt gatctgcacg
     6601 acctcggcgt gaccggtggt gtgggtgcag acctcccggt aggtggggtt ctccgtgtgc
     6661 cccccgctgt agcccgagac gacgtcgatc acgccgggta cctgccggaa cacccactcc
     6721 accccccaga agcagccggc cccgagcgtc gcggtctgga tcgccatggt gaggatcact
     6781 ccttccggat cgatactccc ttcaaccatc cgcccgccgg ccggtgttcc accagcagcc
     6841 agcggctccc atcgaagtcg ctgggtcccg gtcccgaggc gtgccccgat gcccacggcc
     6901 gccgcgatca gcgcgtccgc gacatccccg ccgtccctct gcggcagatc gcctaccgac
     6961 cggtgagcca gtcggccgcc tccttggcgt ggtaggtgag gatgaggtcg gcaccggccc
     7021 ggcggatcga tgtcaggacc tccagcgtgg ccctgcggcc gtcgagccat ccgttctggg
     7081 cggccgcctt gaccatggag tactcccccg agacgttgta ggcggccaag gggaaccggg
     7141 tctcctcctt ggcccgacgg atcacgtcca ggtagggcag cgccggcttc accatgacga
     7201 tgtcggcccc ttcctcgatg tcggtcgtga tctcgcggat cgcctcgtcc gagttcggcg
     7261 ggtccatctg atagccggtg cggtccccga accgcggcgc gcactcggcg gcttctcgga
     7321 acggaccgta gaaggaggag gcgaacttgg ccgcgtaggc cacgatcccc acgcgctgga
     7381 ggccggcctt gtccagcacc tcccggatcg cggcgacctg tccgtccatc atcccagagg
     7441 gcccgagcag gtccgcgccg gcctcggcct gggccacggc gatcttccgg tagcgctcga
     7501 gcgtggcgtc gttgtccacg ttgccctcgt ggtccagcac gccgcaatgg ccgtggtcgg
     7561 tgtattcgtc caggcagagg tcggcgatca cgacgtgatc gtcgccgagc tcctcgcgaa
     7621 gcagcctcag gcccttctga gcgatgccgt ccgggttcca cgcctcggag ccctcgggat
     7681 ccttgcgctc gggcacgccg aagagcatga aggccgtcac cccccgggcg gcgacctcgg
     7741 cggcctcctt ccgcagcgac tcgagggtgt gctggaactg gtgtggcatc gagacgatcg
     7801 gccgtggctc cgcgatgccc tccttcacga acagcggcgc gatcaggtgc cgcggcgcca
     7861 gctcggtctc gcggatcaag gagcggagcg ccggcgtacg acggagccgc cgcggccggt
     7921 gctgcgggaa cgtcgccatg ctcgcgagtg tacgagcccg gcggcgaacg ggcgacccgc
     7981 gatcctgcgt ccgtggctag actcgcggca tcgtgcaacc cgcctacgac gccgcgttgc
     8041 tccgggaggg ggaggcgttc gccgccgcag cccagatggg tctggatctg ccggtgccgt
     8101 cctgcccggg ctggagtgtg gccgagctga cctggcacct gggccgggtc catcgccact
     8161 gggaggaacg ggtccgtctc cggctccagg agcaagccgg cctcaccgag gtcgagcgtc
     8221 ccccgcaccg ggagctggtg ccctggttcc gcgacggtct cgagcgcctg tcgcaggccc
     8281 tccgtggagc ggatccgggg gagcgcatct ggacctgggc caggcagcag gacgtggggt
     8341 tcgtccggcg ccggatggcg caggagaccg tggtgcatcg ggtcgatgcc cagctcgccg
     8401 ccggctcgtt cgaaccgatc gaaccggtcc tggcggcgga cggcgtggac gagttcctgg
     8461 acgtgatgct gggcgaggac gaggagcggc tgagggaggg gacggagacg atccacctgc
     8521 atgccaccga catcgaggcg gagtggctgg tggaggtcgc agacggccgg ctcacgaacc
     8581 ggcacgagca cggcaggggg gaggccgcgg ctcgggcgag cgcctcgggg ttgttgctgc
     8641 tgctctggcg gcggatcgcg ccggaccggg tcgaggtgct gggggatcgc tcggtgctgc
     8701 ggcggttcct ggctcgggcc gacctcggct agggcccctc gctcatccct gacggcgcgg
     8761 cggccggccc accggcgcag gttccccgag cggtcctggc ccggagcgcc cgctccaccg
     8821 cgtcgaccag cccctcgatc gtgtgggggt cggccacccc cgagaccctg aatccgcgcg
     8881 cacgtgcctc gcgggcggtg accggtccga tgcagatcac cttcgggttg ccccgcaagg
     8941 caccgaccgt tcccacgaag ccgcgcacgg tggaagcgct ggtgaacgtg atggcgtcca
     9001 cggacccgtc gcggagcacc ccgcgcgcgg cgcggggcag cgaggtcggg gtccgggtcc
     9061 ggtaggcgtg gacccggacc ggggcccatc ccttcgccgc cagggcgtcc tcgaggccct
     9121 ccggcgcgat gtcggctcgg gcgcagagca cccgcccggt gcctcgcggg aacgtgcgcg
     9181 acagggccgc ggtggtgaag gtccggggca tgaggtcggg gtcgcggccg gtccaggccc
     9241 ggaaggcctc ggcggtgccc tcgccgatca ccgccacgcg ggcccggacg tcctcgggca
     9301 gcagccgggc ctcgagcatg tccacggtgt tcctgctggt gagcgtcagc caatcgaagc
     9361 ggcccatcga gagctcgcga agggccgcgg tcagtgcggc cgagcgcgcg ggcaggatct
     9421 ccacggccgg tgcggccacg gcggtggcac cccgggcctc cagcgcccgg atcagctcgt
     9481 cggcctgggc ccggggacgg gtcaccagga ccacggcgcc gtccagcggc ccggtcacgc
     9541 tcgtgcctcg gccaggatct gctccgcccc ctcggaacgg agacgctggg cggccacgtc
     9601 ggcggccacc tcgggggacg tggcctccac gtcgaccctc gcggcccggt cgccgtcgga
     9661 ggtgaggacg accgcgacca gccggacccg ctggtcctgg acccgagcgt aggccccgag
     9721 gggcagggcg catcccccgc ccaggagccg caccaaccgc cgttccgcct cgaacgcggt
     9781 ccggctgtcc cgatggtcca ggcgcgagag cacctccagg acgccggggt cggctgaacg
     9841 ggcctggatc cccaagcagc cctgacccgg agcggggacc atctccgccg ccgacatcgg
     9901 agcggcatgc tcgggcacga tccccatccg caggagcccg gccgccgcca gcacgagccc
     9961 gtccacctcc ccgcgctcca gcttggccag ccgcgtgtcc acgttgcccc gcagctcgac
    10021 cacccggaga tcagggcgcc accgcaggac ctgggagcgg cggcggaggc tcgaggtccc
    10081 caggccggag ccctgcggga ggtggccgtc gggggtgatc aggacgtcga gggggctggc
    10141 tcgctcgggc acggcggcca gggccacgcc gtcctcgtcc tcggccggga ggtccttggc
    10201 ggaatggacg gccagatcca cctccccctc gcgcagagct cggacgatgt cactcaccca
    10261 caggcccttc aggcccgccg gggaggcgcc gcccgccccg cctcggtccc cggcggtctg
    10321 catggggacc acctcgggct cggcgccggc gttccgcagg gccctcgcca cggcctccgc
    10381 ttgggccatg gccagacgcg atcgccgcgt gccgatccgc agcttcactc ggagcgcgga
    10441 tccaggccga acaggtcggc gagcatacgg gcgtgcaggt cctccgtgcc gggggtggac
    10501 agctccttca gccgcacgat gggctcgtgg agcagcttgg ccacgatgcc tcgggccatc
    10561 gcctccaccg cgtcccgttc gtcgggggtg aggcgcgaga gctcggctcg gaagcgccgg
    10621 gtctcggccg tcaggacgtc gtccccgcgc tcgcgcaggg cccggatcac cggcgccaga
    10681 cgttcggagc gctgtcggat cgcgaagcga tggagctcct cggccaccac cgcctcggcg
    10741 cgccggatgt cctcggaggc ctcggcggcc ggggtggcga gcgccgttcg cagaccctcg
    10801 atgtccacga gcgtcacgcg gtcgagctcg gccaccgagg gctcgacgtc gcgcggcacg
    10861 gccaggtcga gcacgaacag gggccgggtc cgcccgttca gcgccgcggc cacggtgtcg
    10921 tggtggatca cggcaccggc cgccccggtg gctgagacca gcagatcggc cccggccagg
    10981 gcccccggca ggcccgccag gtcgtccggc tcggccgagg tgcgttcggc caaggcccgg
    11041 gctcgctcca ggcttcggtt cagcacccgc accgggccga cgccgcgcgc ccgaaggtgc
    11101 ttgacggcca gggccgccat ctggccggcc cccaccaccg cggcgctctt gccggccagc
    11161 ccggccaggc gctcctcggc caggtgcgca ccggcctcca cgaacgcgtc cggggcggcc
    11221 cccaccccgg tctcggatcg gacccggcgt ccggctcggg ccgccgcatg gaacagggcc
    11281 gtcagggcgt gtcccgcggc cccctcggcg gcagcccggc gatgggcctc gcggacctgg
    11341 gccaggatct gcggttcgcc caggaccatc gagtccagcc ccgccgcgac ggcgaacagg
    11401 tgctgggccg cggcctcgtc gtagtgcgag tacaggggct cggtcagcag gtcggggtgg
    11461 gcgccccggg actcggccag caggcgcttc agggagagga agccggcgtg ataggaaggg
    11521 acgctggcgt agatctccac gcggttgcag gtggagagga tcacggcctc gtccagcgcg
    11581 tcgtcgtcct gtgcgcgccg gtaggccttc gtgaagtcgt cgtcggtgaa cgcgagctgc
    11641 tccagcagct ccacggtcgc gcggcgatgc gagacgccga gagcaaggat cgccatgggt
    11701 acctgctgat tctacggagc atggcgcgga acggtcgtgc ccttgggtcg cagcgcctcg
    11761 aaagcgccgc ccctgcgata gccttgagcg ctcgcgggac gagacgaggg gacggccgtg
    11821 gaacggggac gcgccaggga cctgggggtg gtggtgggca tgctacccac cggtgcccgc
    11881 aacgcgatca ccgacgtggc gggcgtgcgg gtgggacacg ccaccctgat ccgcgacccg
    11941 gaacccggtg ggcggggggc ggttcggacc ggggtcaccg ccgtcttccc ccaccagggc
    12001 ttgccctggg tcgagcgcgt gtacgcgggc acccacatcc tgaacggcta cggcgagctg
    12061 atcggcatca accagatcgc cgaatggggg ctgctgcact cgccggtggt gatgacctcg
    12121 tccctggcca tcgggcgggc ctacgacgcg acggtccggt ggcgtaccga gcggcacggc
    12181 gtgaccacgc acgacggggg ggatatgccg atcgtcaccg agtgcgacga ctcgttcctg
    12241 aacgacgtca cgaccttccc cctgcgtgat caggacgtgt tcgcggcgct ggactcggcc
    12301 gagagcggtg acgtggccga gggctgcgtg ggggccggga ccggcatgca gtgcttcgac
    12361 ttcaaaggcg ggatcggcac ggcctcgcgg gtcctgccgg ccgggctcgg cgggtacacg
    12421 gtgggcgcgc tcgtcctcac gaacttcggg gcccgggagg agctgatcgt ggacggggtc
    12481 ccggtgggcc gggagatcag cgacctgatg cccgaggagc acggcgaggg atcgtgcatc
    12541 tgcctggtgg cgaccgatgc gcccatgctg ccgcatcagg tccgccgcct ggccgtgcgg
    12601 gccggcctgg gcctggcccg gaccggctcg accgcccgga acacctccgg ggagcagatg
    12661 ctggccttct ccaccgccaa ccggcttccg atggagacgc ccgccggcgc cctggaagtg
    12721 cgggcgctgt tggacggagc ggccgagtcc tccccgccga tcctgtccgg gctgttctcg
    12781 gcggtgatcg aggcggtgga ggagggcgtg ctgaacgcct tgttcacggc caccaccacc
    12841 accggccggg acggcaacac gctgcatgcc ttgcccctgg accgcacgct cgggctcctc
    12901 gagcggcacg gccggctccg gtccgcctga acggcgcacc gcgctcccga tggtccctgc
    12961 gggggtgggt cgcatgcccc ctgccgcccc atgctcccca ccgcacgatg gcctcgaggg
    13021 aggaggagcg cccatggatg tcaacggcgg gttccgcagg atgctccagg agcggttcgg
    13081 tcgggtccgc gaggcgatga ccagcagcgt ggtcgagctg gagccctcgc tcctggcggc
    13141 cgaagcggtg cgtctgttga gcgaggccgg cgtcaccggt gggccggtgg tcgaggacgg
    13201 ccgcgtggtg gggatcgtca cgctctcgga tctaatcggg cgcgagcacc acgccgcggc
    13261 ccacgtgacc ggtcccttcc tgcgcagcga ccggcagctc gggaacctca aggtgaccga
    13321 tgtgatgacc cgcgaggtgg tcacggcgtg cgaggactgg ccgctggtgc gggccatcgt
    13381 gaccatggac gacgcaggcg tgaaccggct gcccgtgttg gatgcgttcg atcacccggt
    13441 cgggatcctg gcccgggacg acgtggtgcg ggccgtggcc gcggccctgc gatgggccga
    13501 gcgcgccgaa gagcatggcg cccaggtccc cgactggacc ctgctcgagg ccgactgatt
    13561 cccgatcgcg ggatctgccg gtggacgggt cccgtacgat aggcgaccgg agacgtggct
    13621 gagtggccga aagcagccgc ctgctaagcg gttaggggtg tgaaagcccc tcgcaggttc
    13681 gaatcctgcc gtctccgctg atcgaacgag ttcgagtcac gcccgcgacg gttccgaacc
    13741 cgcttcctca caagccgtta ctcccgtgcg cgatggttta tcgcgcacgc atccggggga
    13801 ctccctctgc aggccggcag gacggagagc cggcatgagg ggagggtaac gacatgacct
    13861 atcacgaccc gcacgaaccg gtgcgagaac ccgactcggg cggtctggtg gggttcgccg
    13921 cgatcaagta cacggccatc gtggtgatcg tcctggcgat cctcgcgttc atcgccttct
    13981 acctgatccc ggcggtgacg agctgatggc cgccgcgatc gtgggtctgc tcgccgccgg
    14041 agcggcgtgg gcgacgatcc tgtggatcat cgccgccgtc ttcgtgatct ggggcatcgt
    14101 cacgctgatc cgaggcgggg tgctggcagg gatcctgttg atcgtcgtgg ggctcctgat
    14161 cggcccgggc ggcgtgagca tcttcaccta gcaccgccag ggtgctcgtc gtcgttcccc
    14221 gctcctatgc tccaggccat ggagcgtagg gcggggttcg tcgaccggat cggagcgctc
    14281 cggccgctgc agatcaggga cttccgcctc ctctggaccg ggctcaccgt gtcgatgatc
    14341 ggcgacggcg tctacgtggt cgccgtggcg ctccaggtac tcgatctgtc gaacagcccc
    14401 acggccatcg ccggcgtggg gatcgcctgg accgcgccgc aggtggtgct gatgctcggc
    14461 agtggagcgc tcgccgatcg catcgatcgc cggcggctga tggtgagcgg cgacctgatc
    14521 cgcctggtgg cgatcggcac gctcgggacg ctgtcgatcg cggaccggct cacgctgccg
    14581 atgctgttcg ccctcgtggt ggtctacggt gtgggacagg cggtcttcgc ccccgccttc
    14641 tcctcgatca tccccacgat cgtgcccgac gacctcctgg tgcaggccaa ctcactgggt
    14701 caggtggttc gcccggccgc catgatgctg atcggcccgc tggtcggcgg gatcctggcc
    14761 gggagcctgg gtccgggctg ggccttcgtg atggacggag ccagcttcgg ctggtcggcg
    14821 gtgatgatcg tgctgatgcg ggtccggccc ctcccccgaa ccgaggaacc gacgcgcgtg
    14881 tgggccgacg tcaagcaggg cctgaggtac gtgcggagcg agacctggat ctgggcggcg
    14941 ctcgtggcgg ccacggtcag cctgctctgc acctggggac cgtgggaggc tctggtgccc
    15001 ttcgtggtgc ggaaccagct cgcgccctcc gggggcgccg gcgtcgcgct ggggctggtc
    15061 ttcggagcgg gtgggttggg agcggtaggg gcggcgctcg tgctcggtca gcgggaaggc
    15121 ctgccccgca agcccgtcac ggtcctgtac ctggcgtggg cgctggggat ggtgatgacg
    15181 gcggggttcg ggatggtggt caccgtgtgg caggggatga tcgtggcctt cgtgtccgag
    15241 ggctcgatcg ccgtgctggt cgtgatctgg tacacgctgc tgcagcgcct ggtcccccgc
    15301 gatctgctgg gtcgagtctc gagcctggac tggctgatct cgatcgccgg ggtgccgctg
    15361 tcgtacgcga tcgtcggccc gatggcaggg tgggtgggcg cggacgccac gctgatcggg
    15421 gcgggcctgc tgggcggggc catcacgctc gccttcatgt tcatcccggg cgcgcgaagc
    15481 cccgaacggg atgggtccct cgagccgcgg cacgagggcg ccgcggtgcg gtcgtgaacc
    15541 ggtggggacg gccggtcgag acgtgatgcg gggccggacc gtccgccgcc tcgttcctcg
    15601 gcccctgggc aacgacgtcg tccatgccgg cggtacgaga cgc
//
LOCUS       DASQQQ010000106         3554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_34850, whole genome shotgun sequence.
ACCESSION   DASQQQ010000106 DASQQQ010000000
VERSION     DASQQQ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34850"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2036)
                     /locus_tag="VE646_04440"
     CDS             complement(<1..2036)
                     /locus_tag="VE646_04440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HZD79273.1"
                     /translation="MAAPPKARRGPKHASRTTKTQQRRGFFRRYWWLFVAVPLGGLLI
                     LVGTLTFVYSRLQLPQTPPPLQTTHLYDRTGKHLLATLHASVDRTIIPFDQMPASVRN
                     AVIAVEDQDFYSHPGIDPLGIVRAAWSDLVSHQVVQGGSTITQQLVKNVYAGHYEHDP
                     KTGQTTYVVPPRTFGQKVREALLAIKLEQSYTKDQILTTYLNTIYFGHGAYGVEAAAQ
                     TYFGKHAAELTLVQSATLAGLIRNPSSFDPIVNPGNAFVRRNYVLERMAQVGDITQAR
                     ADALERTKVKAVKGGSELSFPPKLGYFLDYARRTLIDRFDEGTVFGGGLHVTTSLDER
                     MQHAAEAAVAGRLRSKGDPEAALVAVDPSTGEVRAMYGGRNFGASQVNLALSGAKGFG
                     GTGRQAGSAFKPFTLAAAMEDHVSLSSRWRGPGRITIPDRRCYTDGKPWTLSNASDEE
                     SGDFTLAQATAHSVNTVYAQLVSMVGPDRVVDVAHRMGIRSPLKPVCSITLGTQSVNP
                     LEMTNAYATLAARGVYRPATPLRQVTTPGGQTELKSSRKGRRALDQNDADLVTYALRG
                     VIAGGTGTNANIGRPAAGKTGTAQSYVDAWFCGYVPQLATCVWVGYPKGEIPLTYVEG
                     FPAVYGGTIPALIWHDFMSEAVGSMPVRDFATPSFSGYTRGAPTPPPSLAPSKSP"
     gene            complement(2140..2514)
                     /locus_tag="VE646_04445"
     CDS             complement(2140..2514)
                     /locus_tag="VE646_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HZD79274.1"
                     /translation="MRSRRSLLATVDYTLAKRALLRDVRTGLKSRADICDAHPELMRA
                     ARHVGEPTHRDCPVCERTKLVLLAYVYADDLKQDNGRVWPLDQGLSLAADHRNASCYV
                     VEVCTGCQWNHLSEAFVARSAG"
     gene            complement(2511..2987)
                     /locus_tag="VE646_04450"
     CDS             complement(2511..2987)
                     /locus_tag="VE646_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3654c family TadE-like protein"
                     /protein_id="HZD79275.1"
                     /translation="MSGNEHGSVTIVAAALLAMVVVLTMGASDLGRVLLASSRAQTAA
                     DASALAAAQQVALPSELAPQGVAAEYAAWNRGQLVSCLCDPPTLEATVTVRVPVHGLF
                     LVPGTRYAVASARAVVRREGPLTGVRSVAAGQPVGNLLWGPGRTMPQPWRSTLAGR"
BASE COUNT          542 a         1212 c         1264 g          536 t
ORIGIN      
        1 gggctcttgg agggcgcgag gctcggcgga ggcgtaggcg cccccctcgt gtagccggag
       61 aacgacgggg tggcgaagtc ccgcaccggc atgctcccga cggcctccga catgaagtcg
      121 tgccagatca gcgcggggat cgtgcccccg tacacagccg ggaagccctc cacgtacgtg
      181 agggggatct cgcccttggg atagccgacc cacacgcagg tggccagttg gggcacgtag
      241 ccacagaacc acgcatccac gtagctctgc gcggtcccgg tcttgcccgc cgccggccgt
      301 ccgatgttgg cgttggtccc ggtcccgccg gcgatcacgc cgcggagcgc gtacgtgacc
      361 aggtcggcgt cgttctggtc cagggcgcgc ctgcccttgc gcgaggactt taactcggtc
      421 tgtccgccgg gggtggtcac ctgacgcagc ggggtagccg ggcggtagac gccgcgggca
      481 gccagcgtcg cgtacgcgtt cgtcatctcc agagggttca ccgactgcgt gcccagggtg
      541 atggaacaga ccggcttgag gggggagcgg atccccatcc gatgagccac gtccaccacg
      601 cggtcgggcc ccaccatcga cacgagctgc gcatagacgg tgttcacgga atgggcggtg
      661 gcctgggcca gggtgaaatc gccgctctcc tcgtcgctcg cgttggagag ggtccagggc
      721 ttgccgtccg tgtagcagcg ccggtcggga atcgtgatcc tgcccgggcc ccgccatctc
      781 gagctgaggg agacgtggtc ctccatggcg gccgccagcg tgaacggctt gaaggcggaa
      841 cccgcctgcc ggccggtgcc gccgaagccc ttcgcacctg agagcgccag gttcacctgc
      901 gaggctccga agttgcgccc cccgtacatc gctcggacct cgcccgtgga gggatcgacc
      961 gccacgagcg cggcctccgg atcgcccttc gagcggaggc gaccggccac ggccgcttcg
     1021 gccgcgtgct gcatccgctc gtccagcgag gtggtcacgt gcaggccgcc cccgaacacg
     1081 gtgccctcgt cgaatcggtc gatcagggtc ctgcgcgcgt agtcgaggaa gtagccgagc
     1141 ttggggggga agctgagctc ggatccgccc ttcaccgcct tgaccttggt gcgctcgagc
     1201 gcgtccgccc gggcctgcgt gatgtccccc acctgtgcca tacgttccag cacgtagttg
     1261 cggcgcacga acgcgttccc cgggttcacg atggggtcga aggaggaggg gttcctgatg
     1321 aggccggcca gggtggccga ttggaccagg gtgagctcgg ccgcgtgctt cccgaagtag
     1381 gtctgcgccg ccgcctccac accgtaggcg ccgtgtccga agtagatcgt gttcaggtac
     1441 gtggtcagga tctggtcctt cgtgtagctc tgctcgagct tgatcgccag cagggcctcg
     1501 cggaccttct gcccgaaggt ccggggtggg accacatagg tggtctgccc ggtcttggga
     1561 tcgtgctcgt agtggcccgc gtagacgttc ttcaccagct gctgggtgat ggtggatcca
     1621 ccctgcacga cctggtggga gaccagatcg ctccatgccg cgcggacgat ccccaggggg
     1681 tcgatacctg gatgggagta gaagtcctga tcctcgacgg cgatcaccgc gttcctgacg
     1741 ctggccggca tctgatcgaa ggggatgatc gtccggtcca ccgacgcgtg cagcgtcgcc
     1801 agcaggtgct tgcccgtccg gtcgtagagg tgggtggtct ggagcggcgg gggcgtctgc
     1861 ggcagctgca ggcgcgagta cacgaaggtc agcgtgccga cgaggatgag caggccgccc
     1921 agcggcacgg cgacgaacag ccaccagtag cgacggaaga agccgcgacg ttgctgcgtc
     1981 ttcgtcgtcc gggaagcgtg cttcggcccg cggcgggcct tcgggggtgc ggccaaggcg
     2041 tgccgattct accggcagac ctgcatcatc acgtccggtg cgcgcggcgg gcgtgcggtt
     2101 ccggtgatgg cgccgggtgt cgggccgacc ggggagcgat cagccggcgc tgcgggcgac
     2161 gaacgcctcg gacaggtggt tccactggca tcccgtgcac acctcgacca cgtagcagga
     2221 ggcattgcga tgatcggcgg ccaaggagag cccctggtcg agcggccaca cccgtccgtt
     2281 gtcctgcttg aggtcgtcgg cgtacacgta ggcgagcagg accagcttcg tccgctcgca
     2341 caccggacag tcccggtgcg tgggctcgcc cacgtggcgg gcagcccgca tcagctcggg
     2401 atgggcgtcg cagatgtcgg cacgggactt gagcccggtg cggacgtccc ggagcagggc
     2461 ccgcttggcc agcgtgtaat cgacggtggc caacaacgac cgccggctcc tcaccttcct
     2521 gcgagggttg atcgccatgg ttgtggcatt gtacgccccg gcccccacag aaggttcccg
     2581 acgggctggc cggccgccac ggatctcacc ccggtgagcg gaccctccct ccgaaccaca
     2641 gcccgagcgc tggccaccgc gtacctggtg ccggggacca ggaagagacc gtggacaggc
     2701 acccgcacgg tgacggtcgc ctcgagggtc gggggatcgc acaggcagga caccagctgg
     2761 ccccggttcc aggcggcata ctccgcggcc acgccctgag gggcgagctc ggaggggagg
     2821 gccacctgct gggcggcggc cagcgccgag gcgtccgccg ccgtctgggc cctgctcgac
     2881 gcgaggagca cccgacccag gtcggaggcc cccatcgtga ggacgaccac catggccagg
     2941 agagcggcgg ccacgatggt gaccgacccg tgttcgttgc cgctcacccg aactcctgac
     3001 gcatgatcgc cgtcgcctgc aggtggacct cggacgggaa caggacgccg gccagaggaa
     3061 ccggacggac tcgacgtagc tgacctcgac ccggacgggc tcccccaagc ccccggacgg
     3121 ttgcacgctc acctcctcgt caccctcccg cagcccgggc aaggccacca ccacgtccgg
     3181 ttccacctgc ctgatggcct cgagcagctg atgccggtcc cgcgccgcgg ccaccacgcg
     3241 tggacccggt ccgatcgagg aagtgcatga cctcctcggc cacctcgtcg ccctcgacgg
     3301 ccagcacgat cctggacagc ttctccatcc acgctccatc cggcatcagg gggaagccac
     3361 ggggaagtgg ttcatcacac gaacgaactc agccggggga ggctttcggg agggagcacc
     3421 ggtccgatgc gcaggccgca gcgtacgtga gcccatcgag ccaggcaatc gccaaggcgg
     3481 cggaatcttc ggcggcgtcg gcgggcggat cggccgccta ccggcggatg gatcacccca
     3541 cgctcggggg aggg
//
LOCUS       DASQQQ010000107         4696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_282319, whole genome shotgun sequence.
ACCESSION   DASQQQ010000107 DASQQQ010000000
VERSION     DASQQQ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282319"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2571)
                     /locus_tag="VE646_04455"
     CDS             complement(<1..2571)
                     /locus_tag="VE646_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZD79276.1"
                     /translation="MDTALKPYSPLGLQMRRISGALVGRPTELAAIRQELVDTRPGRL
                     AAVTLEGEPGIGKTRLLLAVNELAGAEGATPIAVAADEELRGPFLLARSILGAPQAVD
                     AASGTPAEDDLRRSLDILSGRDEAGLETLPADQKLLRTCDLAAIALRSLAAERPLVLL
                     VDDLQWVDEDSLRLLRYVVRADATSPILLVLALRPDEFALVHEAVTLVADMERLGLVR
                     RLKLARFSPAEASEMLQQVLGGPVEGSSAATVHAQAEGVPFILEELARAYRDAGMVQQ
                     IDGKWTLAKNAERLLPSAVKTLISRRAAHLPEDTKQALALAAVLGRRFSLKDLGEVMV
                     RVGEAGEPDLQSLAELLRPAALAGLLLQHPADSAADFSFAHEQVREFAAGTLTAARRR
                     AVHEAIVAMLTAGEPSVESLPLLAHHARAAGDAAVCVRFSLEAATNALGSNAPDEVLR
                     VVELALPTASAAQDRVALLQARDQALEMLRRPEDRLQGLAELSALAEALGDSHLELEV
                     QLRRAAALRLAEQHDQAAQIARGVRSLAEERADRPAEMAACLELGQALLRAPLGETFT
                     PPEIEVDMDGAEEAYQRAVVLSEELGDRGATANALRELGVIDLGRVRSWFVGEVQAGR
                     LYEYLGRLTSGETLPDVAAGTPLELFLPSANEKLERALRLFEEVGDRPGAMSTIIALA
                     YVHWAPDIHMGSGAARHIEEIRRLASRMKTMAKESERALAEAQMVYGAHVFARAKVIP
                     DLAVTRGEEAYRLARVIGDRALEFLAAGGTALALLDLGEVEAACGWLDRAAAAATQSP
                     TPLRARLLEMWRGVADAASGDAAGMRRRFERALELATEQGSAAARCEVLARTALEAAR
                     L"
     gene            complement(2643..3314)
                     /locus_tag="VE646_04460"
     CDS             complement(2643..3314)
                     /locus_tag="VE646_04460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007515639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HZD79277.1"
                     /translation="MTGDERWEDVDRYLRELFAPADRVLEAALRDSEAAGLPSIQVTP
                     TQGKLLHLLARATEARRILELGTLGGYSAIWLARALPPDGALITLEADPRHAEVARAN
                     LDRAGVSGLVEIRVGPALETLPQLVGTGPFDLIFIDADKVGYPDYFGWSLRLAGAGSV
                     IVADNVVRGGAVADPDSEDPNVLAVRRFHELVAAEPRVSATVIQTVGTKGYDGFTLAL
                     VTEPG"
     gene            complement(3357..4556)
                     /locus_tag="VE646_04465"
     CDS             complement(3357..4556)
                     /locus_tag="VE646_04465"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZD79278.1"
                     /translation="MSDGPVRSGPVRRLASIGFRPGDDADERVRKATLTLSTSLITVL
                     STGWTTYYFTLGLPRAAAIPLAYQLVSLVSLAVFARTKRYGGFRTAQLAIMLVFPFLL
                     QWTLGGFVPSGAVALWALSSPLGALMFAGTRQAWPWFAGFVALVVASGPLDRVLEPVS
                     IPASTRAAFFVADVIGVSLTAYLLLQYFVRERERERERSERLLLNVLPAPVAERLKEH
                     HGVIADRFEQATVLFADIVDFTPRSAGARPEEVVRLLDEVFTTFDGLAHEHGLEKIKT
                     IGDAYMVAGGIPVPRQDHLEAVAGMALAMTPAVRAIGGLELRIGIDTGPVVAGVIGRS
                     RFIYDLWGDTVNTASRMESQGIPGRIQVTERVASALEPCFVLEPRGTVEVKGKGPMST
                     WFLVGAR"
BASE COUNT          669 a         1808 c         1607 g          612 t
ORIGIN      
        1 cagccgagcc gcttccaggg cggttcgagc caggacctcg caccgggcgg ccgcgctccc
       61 ctgctcggtg gcgagctcca gcgcccgctc gaagcgacga cgcatccccg ccgcatcgcc
      121 gcttgccgcg tcggccaccc cgcgccacat ctccagcagg cgcgctcgga gcggcgtggg
      181 gctctgcgtc gcggccgccg cggcccggtc caaccatccg caggccgcct ccacctcacc
      241 caggtcgagc agggccaggg ccgtgccgcc cgccgccagg aactcgaggg cccggtcgcc
      301 gatcacccgg gccagccggt acgcctcctc cccccgggtg accgccaggt ccgggatcac
      361 cttggcccgg gcgaacacgt gcgccccgta gaccatctgt gcctccgcca acgcccgctc
      421 gctctccttg gccatcgtct tcatccgcga ggccagccgg cggatctcct cgatgtgtcg
      481 cgcggcgccc gagcccatgt ggatgtccgg cgcccagtgg acgtaggcca gggcgatgat
      541 ggtggacatc gccccgggcc ggtcccccac ctcctcgaac aagcgcaggg ctcgctccag
      601 cttctcgttc gcgctcggca ggaacagctc gagcggcgtg cccgccgcga cgtcgggcag
      661 ggtctccccg gacgtgagcc gacccaggta ctcgtagagc cggccggcct ggacctcgcc
      721 cacgaaccag ctgcggaccc gtcccaggtc gatcacgccg agctcgcgca gggcgttcgc
      781 cgtggctcct cggtccccca gctcctcgga cagcaccacc gctcgctgat acgcctcctc
      841 ggccccgtcc atgtcgacct cgatctcggg tggtgtgaag gtctcaccaa ggggggcacg
      901 gagcagggcc tgaccgagct ccaggcaggc ggccatctcg gcgggacggt cggcccgctc
      961 ctcggccagc gaccggaccc ctcgagcgat ctgggccgcc tggtcgtgct gctcggccag
     1021 gcggagcgcg gccgcacgcc ggagctggac ctcgagctcc aggtgcgagt ccccgagcgc
     1081 ctccgccagg gccgacagct ccgccagccc ttgcaggcga tcctccgggc gacggagcat
     1141 ctccagggct tggtcccgag cctggagcag cgccacccgg tcctgggccg cggaggcggt
     1201 aggcagcgcg agctccacca cccggagcac ctcgtcgggc gcgttcgagc ccagcgcgtt
     1261 cgtggcggcc tccagcgaga agcgcacgca gaccgcagca tcgccggccg cccgcgcgtg
     1321 gtgggccagc agcgggaggc tctccaccga gggctcgccg gcggtaagca tcgccacgat
     1381 ggcctcgtgg acggcgcgac ggcgggcggc ggtgagcgtc ccggcagcga actcgcgcac
     1441 ctgctcgtgc gcgaagctga agtcggccgc cgagtcggcc gggtgctgca gcagcagccc
     1501 ggccagggcc gccggacgca gcagctcggc cagggattgg aggtcgggct cgcccgcctc
     1561 tcccacgcgg accatcacct cgcccaggtc cttcaagctg aaccggcgcc ccagcacggc
     1621 cgcgagcgcc agggcctgct tcgtgtcctc gggcaggtgc gccgctcgcc gcgagatgag
     1681 cgtcttgacc gccgagggca gcaggcgctc ggcgttcttg gccagggtcc acttcccgtc
     1741 gatctgctgg accatcccgg cgtcgcggta ggcgcgagcc agctcctcga ggatgaacgg
     1801 gacgccctcg gcctgcgcgt ggacggtggc ggcgctcgag ccctccaccg ggccgcccag
     1861 gacctgctgg agcatctccg atgcctccgc ggggctgaac cgcgcgagct tcagcctccg
     1921 caccagcccc aggcgctcca tgtcggccac caacgtcacg gcctcgtgca ccagggcgaa
     1981 ctcgtcgggc cggagcgcga gcaccagcag gatggggctg gtggcgtcgg cccggaccac
     2041 gtagcgcagg agccggaggc tgtcctcgtc cacccactgc aggtcgtcca ccagcaggac
     2101 gagcgggcgc tccgccgcca agctccggag ggcgatcgcg gccagatcgc aggtccgcag
     2161 cagcttctga tcggcgggga gggtctcgag ccccgcctcg tcccgccccg acaggatgtc
     2221 gaggctccgg cggaggtcgt cctcggccgg cgtccccgac gccgcatcga cggcctgggg
     2281 cgcgccgagg atggagcggg cgagcaggaa gggacccctg agctcctcgt ccgcggccac
     2341 ggcgatgggg gtcgccccct ccgctccggc cagctcgttc accgccaaga gcagccgggt
     2401 cttcccgatg cccggctcac cctccagcgt gaccgcggcc aggcggcccg gtcgggtatc
     2461 gaccagctcc tggcggatcg cggccagctc cgtgggcctg ccgaccagtg cgccgctgat
     2521 ccgacgcatc tgcaggccga gtggcgagta gggcttaagg gccgtgtcca tggcgcggcc
     2581 cattgtgtca cgcgggcgac gacttccggc ggccggctat ccgggctcgg tgtccgggcc
     2641 ggttatccgg gctcggtgac cagcgcgagc gtgaagccgt cgtagccctt ggtcccgacg
     2701 gtctggatca ccgtggcgct cacccgcggc tccgccgcca ccagctcgtg gaaccgacgc
     2761 accgcgagga cgttcgggtc ctcactgtcc ggatcggcga cggcgccgcc ccgcaccacg
     2821 ttgtccgcca cgatcaccga gccggcgccc gccaggcgca gcgaccaccc gaagtagtcc
     2881 gggtaaccga ccttgtccgc atcgatgaag atcaggtcga acggcccggt gcccacgagc
     2941 tggggcaagg tctccagcgc cgggcccacc cggatctcca ccaggccgct caccccggcc
     3001 cggtccaggt tggcccgcgc cacctccgca tgccgtggat cggcttccag cgtgatcaag
     3061 gcgccgtcgg gcggcagggc ccgagccagc cagatcgcgc tgtacccacc cagggtcccc
     3121 agctccagga tcctgcgggc ctccgtcgcg cgcgccagca ggtgcagcag cttgccctgc
     3181 gtgggcgtga cctggatcga cggcaggccg gccgcctcgc tgtcccggag cgcggcctcc
     3241 aggacccggt ccgccggagc gaacagctcc ctgaggtacc ggtccacgtc ctcccaccgc
     3301 tcgtcacccg tcatggtgct cctctctgct cgctgcagcg gctgccggcg gaaccctcac
     3361 cgtgcgccca ccaggaacca ggtggacatg ggacccttgc ccttgacctc cacggtgccc
     3421 cgaggctcca gcacgaagca cggctcgagc gccgacgcca cccgctcggt gacctggatc
     3481 cggcccggga tcccctggct ctccatcctg ctcgccgtgt tcacggtgtc gccccacagg
     3541 tcgtagatga atctgctccg gccgatcacc ccggccacca cgggaccggt gtcgatgccg
     3601 atgcgcagct cgaggccccc gatcgctcgg accgccggcg tcatggccag cgccatcccc
     3661 gccacggcct ccagatgatc ctggcgcggg accgggatgc cgcccgccac catgtacgcg
     3721 tccccgatcg tcttgatctt ctccagcccg tgctcgtgcg ccagcccatc gaaggtggtg
     3781 aacacctcgt cgagcagacg gaccacctcc tcggggcggg caccggccga tcgcggggtg
     3841 aagtccacga tgtcggcgaa cagcacggtg gcctgctcga accgatccgc gatgaccccg
     3901 tggtgctcct tcagccgctc ggccaccggg gccggcagca cgttcagtaa caggcgctcc
     3961 gagcgctcgc gctcgcgctc ccgctcccgc acgaagtact ggagcaagag gtacgcggtg
     4021 agcgagacgc cgatcacgtc agccacgaag aacgccgctc tggtactcgc ggggatcgag
     4081 acgggctcca ggacccggtc caggggaccc gaggccacca ccagggccac gaagcccgcg
     4141 aaccacggcc aggcctgacg agtgccggcg aacatcaggg ccccgagcgg cgagctcagg
     4201 gcccacagcg ccacggcacc ggagggcacg aaccccccca gcgtccactg cagcaggaag
     4261 gggaagacca gcatgatggc cagctgcgcg gtgcggaagc ccccgtaccg cttggtcctg
     4321 gcgaacaccg ccagggacac cagggagacg agctggtacg cgagcgggat cgccgcggcc
     4381 ctgggcagcc ccagggtgaa gtagtaggtg gtccacccgg tggatagcac cgtgatcagg
     4441 ctggtcgaga gggtgagcgt cgccttgcgc acgcgctcgt cggcatcgtc cccgggccgg
     4501 aacccgatcg aggcgagccg cctgaccggt ccggatcgaa cgggtccatc gctcacgcgc
     4561 agactctagc cggagcccga ccccggggcg tcggttgagg ggtggaccgc gcacacgcta
     4621 gcgtgcaacg cgatggggga cttcggcatc ggctcgtgga tcggtcgccg tgcccgcatc
     4681 gatccggccc ggaccg
//
LOCUS       DASQQQ010000108        15175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_63613, whole genome shotgun sequence.
ACCESSION   DASQQQ010000108 DASQQQ010000000
VERSION     DASQQQ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15175
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63613"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1610
                     /locus_tag="VE646_04470"
     CDS             99..1610
                     /locus_tag="VE646_04470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HZD79279.1"
                     /translation="MAERLTALDASFLYLETPQMHMHVAGLTILDPSTRPDGRLPLDD
                     VRRLLASRLHLAPRLRQRVVMVPGNLGLPLWVDDASFDLDFHLRRAALPSPGGRPELT
                     EYVQRSISRPLDRTKPLWELHVIEGLEDGHVATLFKAHHAMIDGISGMQLSAAVYDLQ
                     PVPPEPSRRPRWVPEPTPSPPDLIRGAIQEQLTHPFRAVTEAADRVARSPAVAALGLS
                     VVATGVRSLVDMGSRPPSSLDVPIGPNRRFAMTEASLERYKAIKDALGGTVNDVVLAV
                     VAGALHRLLRARGEATRGRSVRALVPVSVRHAGDVGFGNRVAPAFLNLPVGRMSPGTR
                     LARIREGTHRLKSSTMAMSADAIIGLGAYAPGGMLAAAARLASYAPVFNLVVSNVPGP
                     QQPMYMAGAKLLANYPAMPIGRSSALSVACTSLGGTMAFGLTGDRDGMPDLDDLAGAL
                     AEALGEMAKAAAVQGSASAPAAVGGSRRASPRRPGPSGPGPRSSGRDRPRAGA"
     gene            complement(1498..2412)
                     /locus_tag="VE646_04475"
     CDS             complement(1498..2412)
                     /locus_tag="VE646_04475"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZD79280.1"
                     /translation="MSSTNLDLPPGRFVDLDGPVHYLEWDGPLERTFLLVHGLGGSHL
                     SWALVGQRLAEHGRVLAVDLAGFGRTPRAGRSSRLSANQVLLARFIDEIVQAPAVVVG
                     NSMGGAVAMLQAAYEPASVDGVVLTASVFPWARGGLPHPLVVTGFALYRAPGVGEWTM
                     RRRFGGLSAERIVRFSFRIITEDPSTVPEELIRAHIDLLEERQPDPDIGPAFLEAARS
                     LLSLGRRGELARRIIESVKAPVLVIHGYGDRLVPVAFAQAEIEHHPNWMVRLLPGVGH
                     VPMIEAPDRWVRAVEDWLDEIPPPRPAR"
     gene            2622..3533
                     /locus_tag="VE646_04480"
     CDS             2622..3533
                     /locus_tag="VE646_04480"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZD79281.1"
                     /translation="MALRAGEPERVGAPSVPSYEGFDYQAFWRDPGRRHLDEAERRVV
                     GRLLPATGRRILDVGCGYGRLTGSYVDRFDEVVLLDAAWSLLEQARDRWGGRVRLIAA
                     DLHALPLAPGSFDAAVLVRVLHHFHDPVSVLRHVRRALTSEATLVFSVGNKRNPRRVG
                     RYLLDRSRPNPFGLGLEPYGSSSFGWHPRDVERLPREAGLSLGPVLGLGVLDKVAGRV
                     GALADAVPPGDMLSGPLGRLGIAPVLFAAANPLGPGPAPEEVGRWLRCPICQRGLDGP
                     GSEGGGYRCASCRRRYPVRGGIADFRL"
     gene            3579..4907
                     /locus_tag="VE646_04485"
     CDS             3579..4907
                     /locus_tag="VE646_04485"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZD79282.1"
                     /translation="MAKVSLRAPLRERAGGQRSVDAEGETVVQVLRSLERLHPRLAGW
                     ILDERGTLREHVGVFVNGEKAAATTRVQEGDTMHIVPSISGGAQDTELLVGTRKGLFV
                     LRGGRGGTMSIAARAFKGSTVEYAVRDPRTGRYLASVTHGQFGPRLFVSDDPTEDWEQ
                     AQGPSFPEDADTAVERIWIVQPGESDGLLWAGVAPAGLFCSEDGGLTWALNRGMWDQP
                     DRPSWQPGAGGLALHSICPWPGDPDRLAIGISAAGVWVTEDGGNSWQRSNAGLVARYL
                     PEEVRPTSTDLCVHNMHRAPLRPERLFLQFHGGVYRSDDAGRSWIDIAGGLPSDFGFP
                     LAIDPHDPDRAFVIPLIADRDRVTPDGRVRVFETGDAGTTWEALDTGLPQQEAYLTIL
                     RQAFAQDGQDPLGLYFGATSGEVFGSVDRGATWRVVAPHLAPVSSVRVAR"
     gene            5026..5814
                     /locus_tag="VE646_04490"
     CDS             5026..5814
                     /locus_tag="VE646_04490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HZD79283.1"
                     /translation="MFGPGSAAWRIGGERLVTVVGGGRALLMQIAHPLVAAGVADHSD
                     FEADPFARLWRTIGVVLDVVFGDHDQAAAAAERVNRIHAEVVGERDGSPYRALEPELL
                     LWVHATLVDTALVTYARFVRPVAPAEGDRYYQEMKRSAALFRVPDDILPPDLPAFRRY
                     LRTEISRLRVGEEARRLAGGILDPPLPWPLAPAGYLLRQATIGLLPARLRSAFGFCWT
                     ENRERVLRASAWIIRRTLPLLPANIVRWPHVRASQARSELSERE"
     gene            6009..6941
                     /locus_tag="VE646_04495"
     CDS             6009..6941
                     /locus_tag="VE646_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZD79284.1"
                     /translation="MPEPARPLGTDDADDLRLYLEAAAREPLLTKEEEVELAIIIEHG
                     KEAEEKLRSGRLRSEKSIMKARQAVKKAEAARQRFIMANLRLVVSVARKYQGQGLPLL
                     DLIQEGNIGLMRAVELFDWRRGFKFSTYATWWIRQGITRAIADRSRSIRLPVHIHDRL
                     RKLNRTRFQFAQSMGREPSRDELAKALDTTVEEIDDLTEMGRREPLSLQSPVGEDTEL
                     GDLIEEVDSDAAFNEVEDQLVREEIGDAVERVLDERERHVIALRYGLENGQPLSLRET
                     GKVLGLSGERVRLIEREALRKLRDSNLISAVTVV"
     gene            complement(6965..8332)
                     /locus_tag="VE646_04500"
     CDS             complement(6965..8332)
                     /locus_tag="VE646_04500"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HZD79285.1"
                     /translation="MRVPGVVFASETLITAILSDRSLSQLANVAGLPGIVQASFGMPD
                     IHLGYGFPIGAVAATDVGAGGVVSPGGVGFDISCGVRLLVSELEEAEVRPGLDRLMSA
                     LEGSVPKGTGEGGVWRLRSERELQGLLVEGARFAVAEGHGEERDLERCEDQGRLPDAD
                     PGQVSAHARQRGLSQVGSLGSGNHFLEVQAVDRIVDPELARGFGLRQGLVCVMIHCGS
                     RGLGHQVCTDHVRTMERVMPRYGIDVPDRQLSCAPVDSPEGRAYLGAMAAATNYGRAN
                     RQLLTETVRRSFDQVLGTRSLRLLYDVSHNLAKLERHRMDGRERLVCVQRKGATLALP
                     PGDERLPPDLRRFGQPVLIPGSMGTASYVLAGLPGSGAFHSTCHGAGRRMSRHAALKE
                     IQGKDLRTQLMREGIAVRAGSLKGLAEEAPFAYKDVEDVVRTCEGAGLARRVARLRPL
                     GVLKG"
     gene            complement(8510..9184)
                     /locus_tag="VE646_04505"
     CDS             complement(8510..9184)
                     /locus_tag="VE646_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZD79286.1"
                     /translation="MGGRARGDLNVWWRVGIVVVGAVARIFFRLRATGTEHVPASGPA
                     ILAGNHVSSLDGIALGLVTGERARRMTRFLAAAEFFAKPQFRWALRLYRQIPLHRGAG
                     DEDALGEAIRTIRRGALAGIFPEGRVNPDPDVLQRGRSGLARLAIPTGAPVVPVGIWG
                     TQHRWPRAGLHAHRPWRPTIVVAYGPPLDPGVDGLSANAVLMFTDRVMHAIAHQVQIA
                     RTMAEG"
     gene            9224..10405
                     /locus_tag="VE646_04510"
     CDS             9224..10405
                     /locus_tag="VE646_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HZD79287.1"
                     /translation="MDARELRRIAADRFDDLVDALREMVDIDSGSYTPEGVNRIADLC
                     QARFEADGWSVRRIPHRPDEGQAQLGDLVVGRLDGSGGPRILMIGHTDTVFDPGTAAD
                     RPFTTHGERATGPGVSDMKSGLVLGFFAVEVLREVGFERFGSITYLCNPDEEIGSPFS
                     RPHIEEAATDADAALVLESGRENGGIVSARKGVSDYRIEVVGRAAHAGVEPHKGRSAV
                     LEAAHKIVALHALNGRWSGVTVNAGIVHGGTRTNVVAERCVVEVDLRSPAEASLREAE
                     AAVEEIAATHTVPDVTVEVSAGKWHRPMERSEAVARLAKVAAEVGRELGLELHEASTG
                     GASDANTTAGHGVPTLDGLGPVGGSAHAPQEWMDLRSVVPRMALLAGIISKLGSRPPR
                     G"
     gene            10418..10900
                     /locus_tag="VE646_04515"
     CDS             10418..10900
                     /locus_tag="VE646_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012688165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HZD79288.1"
                     /translation="MDISTVTFDKPDDVNLILGQSHFIKTVEDLHEALVQSAPGLRFG
                     IAFSESSGPRLIRRSGNDEELIALAVRNAEAIGAGHSFVVLLREGFPINVLNAVKMLP
                     EVCRIYAATANRLEVVVAQTEGGRGILGVIDGGSPLGVETEEDVVERKALLRRFGYKL
                     "
     gene            10908..12032
                     /locus_tag="VE646_04520"
     CDS             10908..12032
                     /locus_tag="VE646_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZD79289.1"
                     /translation="MSTQVRIPSGLIKAVIGAEAAARHLGRAVPAIVYVDANDEDGTN
                     VFTSEQATAILGYEAEGWRIDRDGWMSLVHEEDRPSVLEEYRRSRTEARAFGAAYRLR
                     ASDGSYRWFRDEAVPLAAEGGAPLCWRGVMLDVTGEAQAGQRLAQSEDALRRTLEERR
                     TLLARLEESLEEERRRIASDLHDDPIQVISAADMQLQALGALADGDPEVRRGIDEVHE
                     TLKLAVDRLRQLLSELRPAPLGPEGLEATLRAFLEELRFPVRWSLEVRLNADPPADIG
                     AVLFRIAQEAITNAGKHARAERLRVSVSSRDGGIELAVTDDGSGFDRQQSGTSAPGHI
                     GLATMAERAELAGGWCTVKSSSGGGTSVRAWVPLPSAELR"
     gene            complement(12039..13235)
                     /gene="ugpC"
                     /locus_tag="VE646_04525"
     CDS             complement(12039..13235)
                     /gene="ugpC"
                     /locus_tag="VE646_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017586874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HZD79290.1"
                     /translation="MAEIVLDGVTKVFGSDVVAVSDLSLRIDDGEFMVLVGPSGCGKS
                     TILRILAGLEEVTAGEVVIGERQVTDLPPKERDVAMVFQNYALYPHMTVEQNLGFGLR
                     IRRTGKREMQERVADAARILGLEPLLHRRPGELSGGQRQRVAMGRAMVREPQAFLMDE
                     PLSNLDAKLRVQMRAELARLHERLGTTTVYVTHDQVEAMTLGQRVAVLRDGVLQQVDT
                     PQNLYNHPANLFVAAFIGSPPMNLVEARVEGTTVSFAGYRVPVPADLDLGPEAGRSLI
                     LGIRPSDLEDTAVWSNPDLPSIEVTADVTEELGSEVNVLFTVDAPPIATEETLAAADQ
                     AAEDAPIPLVADGTRAIFCARVDARTTARPGSSVRLSLDPRRFHFFDPETGLSLQARR
                     GVGATT"
     gene            complement(13246..14262)
                     /locus_tag="VE646_04530"
     CDS             complement(13246..14262)
                     /locus_tag="VE646_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HZD79291.1"
                     /translation="MSRPAVLAVDGGGSKVDAAILSRHGEVLGAARIPDPKWDQTGDD
                     LHMESIRSAIYAAADDAALGADGSPLAELGVYCLAGADLPADDRRILRGLARSGFSDE
                     SILRNDTFAVLRAGTDRDWGVGVVCGFGTNCTGVAPNGRIYRFPAIGEVSGDWGGGMD
                     VGSEALWHALRARDGRGPKTELATAVPAHFGLRAPRQVMERLYFRRMEPERLAELPPV
                     VFRSAERGDAVARSIVDRQADEVVLMAGAAIRRLRMASLDVDVVLGGGIFRNRFGPFF
                     DRIEEGLRVVCPAVRVTVVSAPPVVGAAMLGLDRIGASMAIKARVRSTLTHDRLIRRL
                     ERSR"
     gene            complement(14259..14879)
                     /locus_tag="VE646_04535"
     CDS             complement(14259..14879)
                     /locus_tag="VE646_04535"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZD79292.1"
                     /translation="MSDGPRVALTLDAEHPDRPLCPPGNAERLLDALGEASIRVTFFV
                     QGRWATAHPDLARRIGSEGHRVGLHSKYHARMTLFSDAGLERDVVEGQAEVAETTGCD
                     PRPWFRCPFDDGHDDPRVQAALERLGYRDVSWNVAAEDWEPDRTPEAVRRDTVRGALA
                     HGDGAVVLLHTWPGPTGRALPLILQDLRASDVRFVTLDQLEPEQLP"
     gene            complement(14872..>15175)
                     /locus_tag="VE646_04540"
     CDS             complement(14872..>15175)
                     /locus_tag="VE646_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79293.1"
                     /translation="PLELAGPGMAAIVLATEPETRRLDLSLARDLVEAGSSVLVITPD
                     GEAPKGAHAAATGYQDRALMSAVSIVPIQLLSWKLSVLHGRTPGEYTRASKVTTRE"
BASE COUNT         2179 a         5301 c         5517 g         2178 t
ORIGIN      
        1 ggcggccggc gcggctcgcc aggtcctctc cacggagccg ccgatcccct gatgcagccg
       61 ttaccgccgg cgcacacggg cggtacggtt gggttgccat ggctgagcga ctgaccgccc
      121 tggacgcgtc gttcctgtac ctggagacgc cccagatgca catgcacgtg gcgggcctca
      181 cgatcctcga tccgtccacg cggccagatg ggcggctccc actcgacgat gtccgccggc
      241 tgcttgcttc ccggctccac ctagcccctc gcctgcgtca gcgtgtggtg atggtgccgg
      301 gcaacctcgg gcttcccctg tgggtcgacg acgcaagctt cgatctggac ttccacctcc
      361 ggcgggcggc gttaccatcg ccgggcggcc ggccggagct gaccgagtac gtgcagcgct
      421 cgatctcccg ccccctcgac cggaccaagc cgttgtggga gctccacgtg atcgaaggcc
      481 tcgaggacgg gcacgtcgcc acgctgttca aggcccatca cgccatgatc gacgggatct
      541 ccgggatgca gctctccgcg gcggtctacg atctgcagcc cgtgccgccg gagccttccc
      601 gccggccccg atgggtgccc gagcccacgc cgtcgccccc ggacctgatc cgcggcgcga
      661 tccaggagca gctgacgcat ccattccgag cggtgaccga ggccgccgac cgggtcgcac
      721 ggtcaccggc cgtggcggcc ctcgggctgt cggtggtggc gacgggcgtc cggagcctgg
      781 tcgacatggg cagccggccg ccctcgtcgc tggacgtccc catcggcccg aaccggcgat
      841 tcgccatgac cgaggcatcg ctggagcggt acaaggcgat caaggacgcg ctgggtggaa
      901 ccgtcaacga cgtcgtgctg gccgtcgtgg ccggcgccct gcaccggctg ctccgtgccc
      961 ggggggaggc cacccgggga cggagcgttc gggccctggt tccggtctcg gtccggcacg
     1021 cgggggacgt ggggttcggc aaccgggtgg ccccggcctt cctgaacctc ccggtaggca
     1081 ggatgtcgcc cggcactcgg ttggctcgga tccgggaggg cacccaccgc ctgaagagct
     1141 cgacgatggc catgagcgcg gatgcgatca tcggtctcgg cgcctacgcc cccgggggca
     1201 tgctcgcggc ggcagcccgg ctggcttcgt acgcgcccgt cttcaacctg gtggtctcca
     1261 acgtcccggg cccgcagcaa cccatgtaca tggcgggagc caagctgttg gccaactacc
     1321 cggccatgcc gatcggacgc agctcggcgc tgtccgtggc gtgcacctcg ctcgggggca
     1381 cgatggcgtt cgggctgacc ggcgatcggg acgggatgcc cgacctggat gacctggcgg
     1441 gggccctcgc cgaggcgttg ggcgagatgg cgaaagctgc cgccgtccag ggatccgcta
     1501 gcgcgccggc cgcggtgggg ggatctcgtc gagccagtcc tcgacggccc ggacccagcg
     1561 gtccggggcc tcgatcatcg ggacgtgacc gaccccgggc aggagcctga ccatccagtt
     1621 cgggtggtgc tcgatctcgg cctgcgcgaa cgccacgggg accaggcggt cgccgtagcc
     1681 gtggatcacc agcaccggcg ccttcaccga ctcgatgatc cgccgggcca actcgccgcg
     1741 acgacccagc gaaaggagcg atcgagcggc ctccaggaag gcggggccga tatctggatc
     1801 gggctggcgc tcctcgagca ggtcgatgtg ggcgcggatc agctcttccg gcacggtgct
     1861 cggatcctcc gtgatgatcc ggaagctgaa gcggacgatc cgctcggccg agagcccccc
     1921 gaaccggcgc cgcatggtcc attccccgac cccgggggcg cggtacaagg cgaatcccgt
     1981 caccacgagc ggatggggga ggccgccccg agcccatggg aagaccgatg cggtgaggac
     2041 gacgccgtcg acggacgcgg gttcgtatgc agcctggagc atggccaccg cgccgcccat
     2101 cgagttgccc accacgacgg cgggggcttg gacgatctcg tcgatgaacc gcgccagcag
     2161 cacctggttg gccgataggc gactgctccg ccccgcgcgc ggggtacgcc cgaacccggc
     2221 cagatccacc gccaggaccc gaccgtgctc agcgagccgc tggcccacca gcgcccagga
     2281 caggtgcgag cctcccagcc catggaccag gaggaacgtc cgctcgagcg gcccgtccca
     2341 ctccaggtag tgaaccggac catcgaggtc gacgaagcga ccgggaggga ggtcgaggtt
     2401 ggtgctactc atggctcgag ccagcgtacc gttccctccg cgagccgcgg gcccggggca
     2461 ccgagcgtgg aggatcgctg cgatgcactt cagcgccgga tcctccgggg gcgccgagcg
     2521 acgaggtgac gccgtcgtca cgaagtagcc accggacccc aaccggtccc taaccgtggt
     2581 ccgaccgttc cgcaaccgtg cgtgggaatg ctgtgcgagc catggccctt cgcgcagggg
     2641 aaccggaacg ggtcggggcc ccctcggtgc cgtcgtacga ggggttcgac taccaggcgt
     2701 tctggcggga tccgggacgg aggcacctgg atgaggccga gcgccgcgtg gttggtcggc
     2761 tcctccccgc caccggacga cggatcctgg acgtgggctg cggctacggt cgtctgaccg
     2821 gctcgtacgt ggaccggttc gacgaggtgg tcctgctcga cgccgcctgg tcgctgctgg
     2881 aacaagcgcg cgaccggtgg ggcgggcggg tccgtctgat cgcggcggac ctgcacgcct
     2941 tgccgttggc gccaggctcg ttcgatgcgg cggtactggt gcgggtgctg catcacttcc
     3001 acgaccccgt ctcggtgctc cgccacgtgc gccgggccct gaccagcgag gcgaccctgg
     3061 tgttcagcgt ggggaacaag cgcaacccca ggagggtggg gcgctacctg ctggaccggt
     3121 ctcgcccgaa cccgttcggc ctggggctcg agccctacgg atcgtcgtcg ttcggctggc
     3181 atcccaggga cgtggagcgg ctgccccggg aggcggggct ctcgctgggg cccgtgctcg
     3241 ggctgggggt cctggacaag gtggccggca gggtcggggc gttggccgac gccgtgcccc
     3301 cgggcgacat gctttcggga ccgcttggtc ggctcgggat cgcccccgtt ctgttcgccg
     3361 cggccaaccc cctcggtccc ggacctgccc cggaggaagt gggtcgctgg ctgcgatgtc
     3421 ccatctgcca gcgcggcctc gatggccccg gatccgaggg cggtggctat cggtgcgcat
     3481 cgtgccgccg ccggtacccc gtgaggggcg gcatcgccga cttccggctc tgagccggct
     3541 ggcctcgctc ctcggcggtg atggctacac ttccccgcat ggccaaggtc agcctccgcg
     3601 caccgctccg agaacgggcc gggggacagc gatcggtcga cgccgagggc gagaccgtcg
     3661 tccaggtact gcgttccctc gagcggctcc acccccggct ggcgggatgg atcctggacg
     3721 agcgcgggac gctcagggag cacgtggggg tgttcgtgaa cggcgagaag gcggcggcga
     3781 cgactcgcgt gcaggagggc gacacgatgc acatcgtgcc gtcgatctcc ggcggcgccc
     3841 aggacacgga gctcttggtg ggcacgcgca agggcctgtt cgtgctccgg ggtggtcgcg
     3901 gtggaacgat gtcgatcgcc gcccgcgcgt tcaagggcag cacggtcgag tatgcggtca
     3961 gggatcctcg gaccggccgg tacctcgcct cggtaaccca cggccagttc ggccccaggc
     4021 tgttcgtgag cgacgatccg accgaggact gggagcaggc ccaggggccg agcttcccgg
     4081 aggatgccga tacggccgtc gagcgcatct ggatcgtcca gccgggcgag agcgacggtc
     4141 tgctctgggc gggggtggcc cccgcggggc tgttctgcag cgaggacggg ggtctgacgt
     4201 gggccttgaa ccggggcatg tgggatcagc ccgatcgtcc ctcctggcag ccgggcgccg
     4261 ggggcctcgc cctgcattcg atctgcccct ggcccggcga ccccgaccgg ctggctatcg
     4321 gcatctcggc tgcgggcgtc tgggtcacgg aggacggagg gaacagctgg cagcgctcca
     4381 acgcgggtct ggtcgcccgg tacctgcccg aggaggttcg tcccacctcc accgacctct
     4441 gtgtgcacaa catgcaccgt gcgccgctgc ggccggagcg tctgttcctg cagttccacg
     4501 gcggcgtcta ccgctcggac gatgccggga ggtcgtggat cgacatcgct ggggggcttc
     4561 cctccgactt cggcttccct ctggcgatcg acccgcacga cccggaccgg gcctttgtga
     4621 tcccgttgat cgcggaccgg gaccgggtga cgccggacgg gcgggtccgg gtgttcgaga
     4681 cgggtgatgc cggcacgacc tgggaggctc tggacaccgg gttgccgcag caggaggcgt
     4741 acctgacgat cctgcgccag gccttcgctc aggacggaca ggacccgctc ggcctgtact
     4801 tcggggccac ctcgggcgag gtgttcgggt cggtcgaccg cggcgcgacc tggcgggtgg
     4861 tggcacctca cctggctccc gtgtcctcgg tccgggtggc tcggtagacg ggcccggacg
     4921 gccccgacgt agcatcgatc catggggtct gcgacccggc ctcgcctttc ggacgccctc
     4981 ggcggtctac ccaccccccg tcccggacgc cgcggggatc cggggatgtt cgggcctggt
     5041 tccgcggcat ggcgcatcgg cggagagcgc ctggtcaccg tggtgggtgg gggacgggcc
     5101 ctgctcatgc agatcgctca tccactggtg gccgcggggg tggccgatca cagcgacttc
     5161 gaggccgatc ccttcgcgcg gctgtggcgg acgatcggcg tggtgttgga tgtggtgttc
     5221 ggggaccacg accaggccgc cgcggccgcg gagcgcgtga accggatcca tgccgaggtg
     5281 gtgggcgaac gggacggctc cccgtaccga gccctcgagc ctgagctctt gctgtgggtc
     5341 catgccacgc tcgtggacac ggccttggtc acgtacgcgc ggttcgtccg cccggtggct
     5401 ccggccgagg gcgaccgcta ctaccaggag atgaagcggt ccgccgccct gttccgggtc
     5461 cccgacgaca tccttccccc tgacctgccg gcgttccgac ggtacctgcg cacggagatc
     5521 tcccggttgc gtgtgggtga ggaagcacgc cgcctggcgg gcgggatcct ggacccaccg
     5581 ttgccatggc ccctggcgcc cgccgggtac ctgctccgcc aggccacgat cggcctgctc
     5641 cccgcccggc tgcggagcgc gttcggcttc tgctggaccg agaaccgtga gcgcgttctc
     5701 agggcatcgg cctggatcat caggcgaacc ttgccgctgc taccggcgaa catcgtgcgg
     5761 tggccgcacg tgcgggcctc ccaagctcgc tccgagctga gcgagcggga atagcggaga
     5821 cgttcacgat gtctcaacat atgtcggcct ttacaaaacc cgaggccgtg ggtaccatcc
     5881 gcagcgaggg gcctggggcg gccagttctt gtgcgcccgg ggttcaacgt acgtcgtaag
     5941 gtcccgaacc ctcgagctga ggcaggcacg cctcggatcg ggggctcgag gggagagtga
     6001 aaaagcagat gccagaaccc gcacgaccgc tcggcacgga cgacgccgac gacctccggc
     6061 tgtacctgga ggccgccgcg cgtgagcccc tcctgaccaa ggaggaggag gtcgagctcg
     6121 ccatcatcat cgagcacggc aaggaggcgg aagagaagct ccgctccggt cggctgcgct
     6181 cggagaagtc catcatgaag gcgcgtcagg ccgtcaagaa ggcggaggcg gcgcgccagc
     6241 gcttcatcat ggcgaacctc cggttggtgg tgtcggtcgc tcggaagtac cagggacagg
     6301 ggctccccct cctggacctg atccaagagg ggaacatcgg cctgatgcgc gccgtcgagc
     6361 tgttcgactg gcgccgggga ttcaagttct cgacctacgc cacctggtgg atccggcagg
     6421 gcatcacccg ggccatcgcc gaccggtcgc gttccatccg gctgccggtc cacatccacg
     6481 accgactgcg caagctgaac cgcacccggt tccagttcgc ccagagcatg ggtcgggaac
     6541 cctcgcgcga cgagctcgcc aaggccctgg acaccacggt ggaggagatc gacgatctca
     6601 ccgagatggg tcggcgcgag ccgctgtccc tgcagtctcc ggtgggcgag gacaccgagc
     6661 tgggcgacct gatcgaggag gtcgactcgg acgccgcgtt caacgaggtc gaggaccagc
     6721 tggttcgcga ggagatcggc gacgcggtcg agcgtgtgct cgacgagcgc gagcgccacg
     6781 tgatcgccct gcggtacgga ctcgagaacg ggcagcccct ttcgctgcgc gagaccggca
     6841 aggtgctcgg gctgtccggg gagcgggtcc ggctgatcga gcgcgaggcg ctgcgcaagc
     6901 tgcgggattc caacctgatc tctgccgtca ccgtggtgtg agggccgagc gcccgaacga
     6961 ccggtcaacc cttcagcacg cccagggggc gcagccgtgc gacccgccga gcgagcccgg
     7021 ccccctcgca ggtcctgacg acgtcctcga cgtccttgta cgcgaacggt gcctcctcgg
     7081 ccagtccctt gagagagccg gcccgaaccg cgatgccctc gcgcatgagc tgcgtccgga
     7141 ggtccttccc ctggatctcc ttcagcgcgg cgtgtcggct catccgtcga ccggccccat
     7201 ggcatgtcga atggaaggcg ccgctgcccg gcaggcctgc cagcacgtag gaggccgtgc
     7261 ccatcgaacc cgggatcagc accggctgcc cgaaccgccg caggtcgggg ggcaggcgct
     7321 cgtcgcccgg gggaagggcc agggtggccc ccttccgctg cacgcacacc agccgttcgc
     7381 ggccgtccat ccggtgacgc tcgagcttgg ccaggttgtg ggacacgtcg tacaggagcc
     7441 gaagggaccg ggtcccgagc acctgatcga acgacctgcg gacggtctcc gtcagcagct
     7501 ggcggttggc gcgcccgtag ttggtggcgg cggccatcgc gcccaggtac gcgcgcccct
     7561 cgggcgagtc caccggggcg cacgagagct ggcgatcggg gacgtcgatc ccgtacctgg
     7621 gcatcacgcg ctccatggtg cgcacgtggt cggtgcagac ctggtggccc aggccgcgcg
     7681 agccgcagtg gatcatcacg cagaccagcc cctgtcgcag gccgaagcca cgggccagct
     7741 cggggtcgac gatgcggtcc accgcctgca cctcgaggaa gtggtttccc gatcccaggc
     7801 tgcccacctg gctcagcccg cgctgccgag cgtgggcaga tacctggccc gggtctgcgt
     7861 cgggcagccg gccctggtcc tcgcagcgct cgaggtcgcg ctcctcgccg tgcccttcgg
     7921 ccacggcgaa ccgggcgccc tccaccagca atccctgcag ctcccgctcc gaacgcaggc
     7981 gccagacgcc cccttcgccg gtccccttgg ggacgctccc ctcgagggcg ctcatcagcc
     8041 ggtccaaccc cggccgcacc tcggcctcct cgagctcgga gaccagcagg cggacgccgc
     8101 aggagatgtc gaagcccacg ccgccgggcg agaccacgcc accggccccc acgtcggtgg
     8161 cggcgacggc accgatcggg aacccgtagc ccaggtggat gtcgggcatc ccgaacgagg
     8221 cctgcacgat gcccggcagg ccggccacgt tcgcgagctg gctcagcgag cggtcgctca
     8281 ggatcgccgt gatcagggtc tctgaggcga acaccacacc gggcacgcgc atcgcgtccc
     8341 tcggtcggat cgtccatcgg aacggttcct ggcgggtgag ctggaaccgg ccgtccgtgg
     8401 acctgcgggt cgcaggctgc gccggcaagg tgcacttcct ccgtccgacc gtagcacggt
     8461 gccgaggcgg ggggcggtgc ctgcggggct ggtgaccgca ccggcgcgat catccctcgg
     8521 ccatcgttcg ggcgatctgc acctgatgcg cgatcgcgtg catcacccgg tccgtgaaca
     8581 tcaagacggc gttcgcggac aagccgtcca ccccgggatc cagcggaggg ccgtacgcca
     8641 ccacgattgt cgggcgccac ggccgatggg cgtgcaggcc ggcgcgcggc caacgatgct
     8701 gagtgcccca gatcccgacg gggaccacgg gcgcccccgt cgggatcgcc agccgagcca
     8761 ggcccgagcg gccccgctgg aggacgtcgg ggtcggggtt caccctaccc tccgggaaga
     8821 tgccggcgag cgcgccccgc cggatcgtcc ggatcgcctc gcccaacgca tcctcgtcgc
     8881 cggctccccg gtggagcggg atctggcgat agaggcggag cgcccatcgg aactgcggtt
     8941 tggcgaagaa ctcggccgcg gccaggaacc gcgtcatgcg gcgggcccgc tcgccggtga
     9001 ccaggcccag ggcgatgccg tccagcgagc tcacgtggtt gcccgccagg atggccggtc
     9061 cggaggccgg gacgtgctcc gtccccgtgg cgcgcaggcg gaagaagata cgggccacgg
     9121 cccccacgac cacgatcccg acgcgccacc agacgttcag gtcgccccgc gctcgccccc
     9181 ccacgccgcg gatgctagcg gtccctccgt atactggccc gccgtggacg cccgggagct
     9241 ccgacggatc gccgccgacc gcttcgacga tctggtggac gcgctgcggg agatggtgga
     9301 catcgactcg ggctcgtaca cgccggaggg cgtcaaccgg atcgccgatc tctgccaggc
     9361 ccggttcgaa gcggacggat ggtcggtccg gaggatcccc caccgtcccg acgaggggca
     9421 ggcacagctg ggcgacctgg tggtgggtcg cctggatggc tcgggcggac cgaggatcct
     9481 catgatcggg cacacggaca cggtcttcga tcccgggacg gccgccgacc gaccgttcac
     9541 cacgcatggg gagcgagcca ccgggcccgg ggtctcggac atgaagagcg ggctggtgct
     9601 cgggttcttc gcggtggagg tgctgcggga ggtgggcttc gagcgcttcg gctccatcac
     9661 ctacctctgc aacccggacg aggagatcgg ctccccgttc tcccgccccc acatcgagga
     9721 ggctgcgacc gacgccgatg ccgccctggt cctggagagc ggccgcgaga acggtggcat
     9781 cgtctcggcc cgcaagggcg tgtcggacta tcggatcgag gtcgtgggcc gcgctgctca
     9841 cgcgggggtg gagccacaca aggggcggag cgccgttctc gaggcggccc ataagatcgt
     9901 tgcgctgcac gcgctgaacg gccggtggtc gggagtcacc gtgaacgcgg gcatcgtgca
     9961 cggcggcacg cggaccaacg tggtggccga gcgatgcgtg gtcgaggtcg acctccggtc
    10021 gcccgcggaa gcctcgctgc gggaggccga agccgccgtc gaggagatcg ccgccaccca
    10081 cacggttccc gacgtgaccg tggaggtctc ggcggggaag tggcaccggc ccatggagcg
    10141 gagcgaggcc gtcgcacggc tggcgaaggt ggccgccgaa gtcggccgcg agctgggcct
    10201 ggagctgcac gaggcctcca cgggcggagc ctccgacgcg aacaccaccg ccgggcatgg
    10261 tgtcccgacc ctggacggcc tgggcccggt gggcgggtcc gcccacgctc cacaggagtg
    10321 gatggatctg aggtcggtcg ttcctcggat ggcgctgctg gccgggataa tctccaagct
    10381 ggggagccgg ccaccgaggg gttgaggagc gctggccatg gacatcagca cggtgacgtt
    10441 cgacaagccg gacgacgtga acctcatcct ggggcagtcg cacttcatca agaccgtcga
    10501 ggatctccac gaggcgctgg tgcagtccgc gcccggcttg cggttcggta tcgcgttctc
    10561 cgagtcgagc ggccctcggc tgatccgacg gagcggcaac gacgaggagc tgatcgcgtt
    10621 ggccgttcgc aacgccgaag cgatcggggc cgggcactcg ttcgtggtcc ttctgcgcga
    10681 ggggttcccg atcaacgtcc tgaacgcggt caagatgctc cccgaggtgt gccggatcta
    10741 tgctgcgacg gcgaaccggt tggaggtcgt ggtggctcag accgaagggg gacgggggat
    10801 cctgggggtg atcgacgggg ggagcccgct cggcgtggag accgaggagg acgtcgtgga
    10861 gcgcaaggcc ctgctccgtc ggttcgggta caagctgtga ggcggcgttg agcacccagg
    10921 tgaggatccc gtccggcctc atcaaggccg tgatcggcgc agaggccgcg gcgcggcacc
    10981 tgggtcgcgc cgtgccggcg atcgtctacg tcgacgcgaa cgacgaggac ggcaccaacg
    11041 tgttcaccag tgagcaggcg accgccatcc tcgggtacga ggcggagggc tggaggatcg
    11101 accgggacgg gtggatgtcg ctggtccacg aggaggaccg ccccagcgtc ctggaggaat
    11161 accgccgatc gcgaaccgag gctcgggcgt tcggcgccgc gtaccggctg cgggcctccg
    11221 acgggtccta ccggtggttc cgggacgaag ccgttccgct cgccgcggag ggcggagcgc
    11281 ccctgtgttg gcggggggtc atgctggacg tcaccgggga ggcgcaggcg gggcagcgcc
    11341 tggcccagag cgaggatgcc ctccgcagga cgctggagga acggcggacc ctgctggcca
    11401 ggctcgagga gtctctggag gaggagcggc gcaggatcgc ctccgacctg cacgacgatc
    11461 cgatccaggt gatcagcgcc gcggacatgc agctccaggc cttgggagct ctggccgacg
    11521 gcgatccgga ggtccggcgc gggatcgacg aggtccatga gacgttgaag ctcgccgtcg
    11581 accgcctgcg ccagctgctg tcggagctcc ggccggcgcc gctgggcccg gagggcttgg
    11641 aggccaccct gcgagccttc ctcgaggagc tccgcttccc ggtgcgctgg agcctcgagg
    11701 tccgcttgaa cgcggacccg cccgcggaca tcggagccgt gctcttccgg atcgctcagg
    11761 aggccatcac gaacgccggc aagcatgcgc gggcggagcg gctgcgggtg tccgtctcgt
    11821 ccagggacgg ggggatcgag ctcgcggtga ccgacgacgg gagcgggttc gatcggcagc
    11881 agtcggggac gtcggcaccc gggcacatcg gcctggcaac catggcggag cgggccgagc
    11941 tcgcgggcgg ctggtgcacg gtgaagagca gttccggagg ggggacctcc gtgcgggcat
    12001 gggtcccgct tccctccgcc gagcttcgct gatcgacctc acgtcgtggc acccacgccc
    12061 cgccgtgcct ggagggacag tcccgtctcc gggtcgaaga agtggaaccg gcgggggtcc
    12121 agcgacagac ggaccgatga tccgggacga gcggtggtcc gggcatcgac ccgtgcgcag
    12181 aagatcgccc gggtcccgtc cgccacgaga gggatcgggg cgtcctcggc agcctgatcg
    12241 gccgcggcca gcgtctcctc cgtggcgatg gggggcgcgt cgaccgtgaa cagcacgttg
    12301 acctccgagc cgagctcctc ggtgacgtcg gccgtgacct cgatcgacgg gaggtcgggg
    12361 ttcgaccaga cggccgtgtc ctccaggtcg gacggccgga tccccaggat cagggagcgt
    12421 ccggcctccg ggccgaggtc gaggtccgcc ggcacgggga cgcggtaccc ggcgaaggaa
    12481 accgtggtcc cctccacccg cgcctccacc aggttcatcg ggggtgagcc gatgaaggcg
    12541 gccacgaaca ggttcgcggg gtggttgtag aggttctgcg gcgtgtcgac ctgctgcagg
    12601 acgccatcgc ggagcacggc cacacgctgg ccgagggtca tggcctcgac ctgatcgtgc
    12661 gtgacgtaca cggtcgtggt ccccagccgc tcgtggagtc gggccagctc ggcgcgcatc
    12721 tgcacccgta gcttcgcgtc gaggttggag aggggttcgt ccatcaggaa ggcctgcggc
    12781 tcccgcacca tggcccgtcc catggccacg cgttgccgct ggcccccgga gagctctccc
    12841 ggccgccggt gcagcagggg ctcgagcccg aggatccgcg cggcgtccgc cacccgctcc
    12901 tgcatctccc tcttgccggt ccgacggatc cggaggccga agccgaggtt ctgctccacg
    12961 gtcatgtgcg gatagagcgc gtagttctgg aacaccatcg cgacgtcgcg ctccttcggc
    13021 gggaggtccg tgacctgccg ttcgccgatc acgacctcgc ccgcggtgac ctcttccagg
    13081 cccgccagga tccgtaggat cgtcgacttc ccgcatcccg agggccccac caggaccatg
    13141 aactcgccgt cgtcgatccg cagggagagg tcgctcacgg cgaccacgtc cgatccgaag
    13201 accttcgtca caccgtccag gacgatctcc gccatgtcgt gcctcctacc tcgatcgctc
    13261 gagtctccgg atcagccggt cgtgggtcaa ggtgctccgc acgcgtgcct tgatggccat
    13321 cgatgccccg atacggtcga gcccgagcat ggccgcaccc accacgggag gagccgacac
    13381 caccgtcact cggaccgccg ggcagaccac acggagaccc tcctcgatgc ggtcgaagaa
    13441 gggtccgaag cggttgcgga agatgccgcc accgagcacc acatccacgt cgagggaggc
    13501 catgcggagc cgccggatcg ccgccccggc catcagcacc acctcgtcgg cctgccggtc
    13561 cacgatgctc ctggcgacgg cgtctcctcg ctcggcggag cggaagacga ccggcggcag
    13621 ctcggccagg cgctccggct ccatccgccg gaagtacaga cgctccatca cctgccgggg
    13681 cgccctgagc ccgaagtggg cgggcacggc cgtggccagc tcggtcttcg gtccgcggcc
    13741 gtcgcgggcg cgcagggcat gccagagggc ctcgctgccg acgtccatcc cgccacccca
    13801 gtcgccggag acctcgccga tggccgggaa ccggtagatg cggccgttgg gcgccacacc
    13861 ggtgcagttc gtgccgaacc cgcagacgac cccgaccccc cagtcgcgat ccgtgcccgc
    13921 ccggagcacg gcgaacgtgt cgttgcgaag gatgctctcg tcgctgaacc ccgagcgggc
    13981 cagccctctg aggatccgcc ggtcgtccgc ggggaggtcc gcccccgcca ggcagtacac
    14041 gccgagctcg gccaggggcg agccgtcggc cccgagcgcg gcatcgtcgg cggccgcgta
    14101 gatcgcggag cggatcgact ccatgtgcag gtcgtcgccg gtctggtccc acttgggatc
    14161 ggggatccgg gcggcgccca ggacctcgcc gtgccgggac aggatcgccg cgtccacctt
    14221 cgaaccgcct ccatcgacgg ccagcacggc gggccggctc acggcagctg ctccggctcc
    14281 agctggtcca gggtgacgaa ccggacgtcg gaggcccgca ggtcctggag gatcagcggg
    14341 agcgcccggc cggtcgggcc gggccacgtg tggagcagga ccacggcgcc gtcaccgtgg
    14401 gcgagcgcac cgcgcaccgt gtcccgccgg acggcttccg gcgtcctgtc cggctcccag
    14461 tcctcggccg cgacgttcca cgacacgtcc cggtacccga gccgctccag ggccgcctga
    14521 acccggggat cgtcgtggcc gtcgtcgaag gggcaacgga accagggccg ggggtcgcag
    14581 ccggtggtct cggcgacctc cgcctggcct tcgaccacgt cccgctccag gcccgcgtcc
    14641 gagaacaagg tcatcctcgc gtggtacttg gaatgcaggc cgacccggtg gccctcgctc
    14701 ccgatccgcc gggccaggtc cggatgggcg gtcgcccagc gaccctggac gaagaaggtg
    14761 acccggatcg atgcctcgcc caaagcgtcg agcagccgct ccgcgttgcc cggcgggcac
    14821 aggggacgat cggggtgctc ggcgtcgagg gtgagcgcga cccgagggcc gtcactcacg
    14881 ggtggtcacc ttggatgccc gcgtgtactc gcccggcgtg cgcccgtgga gcaccgagag
    14941 cttccacgag aggagctgga tcggcacgat ggagacggcc gacatcaggg cccgatcctg
    15001 gtacccggtg gcggccgcgt gggcgccctt cggtgcctca ccgtcggggg tgatgaccag
    15061 caccgagctg ccggcctcca ccaggtctcg cgccaggctg aggtccagcc ggcgtgtctc
    15121 gggctcggtg gcgagcacga tcgccgccat gccggggccc gcgagctcga gggga
//
LOCUS       DASQQQ010000109         9672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_63755, whole genome shotgun sequence.
ACCESSION   DASQQQ010000109 DASQQQ010000000
VERSION     DASQQQ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9672
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63755"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="VE646_04545"
     CDS             <1..965
                     /locus_tag="VE646_04545"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HZD79294.1"
                     /translation="PSKGTGTAPGVGPSATGGASATGGSQAARQPRHLPAPPDIRPIA
                     ANPLPHEGLWQTVGRTADGIPTLRVAFLRPDPVHTSLVAGVAWMDTTLLRAALVAGTQ
                     VPGGSAWQQGWGAEVPRSRRGSLVATFNSGFLLKDSHGGYYADGRTYAPLVPGGASVV
                     VDRDGAITVGRWGVDVQMGPQVAFVRQNLQLIVNHGRPVPGLATDSYQRWGATLGNAV
                     MVWRSGLGVTTDGALVYAAGPGLSVQSLAAVLARAGAVRAMELDINTDWVSFNYYTQN
                     RSSSWGVAAHKLLSTMIRSQDRYLVPDERDFFALSLRPRFAKTG"
     gene            complement(977..1783)
                     /locus_tag="VE646_04550"
     CDS             complement(977..1783)
                     /locus_tag="VE646_04550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZD79295.1"
                     /translation="MNKGFILPGGSASEQLEQAMMAEDAGWDGIFVWEAAYGIDAWSL
                     LAAMAVRTRTIKLGTLLTPLPWRRPWKLASQAATVDQLSEGRTILTVGIGAVTDDLPL
                     TGEVTGVRERAELMDEGIDLLRTLWSGQGHHEGPHYRYECAMDDQIEIGRPVQHRIPI
                     WVVGVWPRPRSMARVLRCDGIVPQYSDHEGTPARAREVRAWLSEHGASDLDLIADGET
                     PEDPALAGEVVGPWAEAGCTWWLETNWEMPHHVPERMQQVRDRIAAGPPA"
     gene            1776..2108
                     /locus_tag="VE646_04555"
     CDS             1776..2108
                     /locus_tag="VE646_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79296.1"
                     /translation="MFIGAAPLRHDVTTSTGSPAHGSRSRTVARDRRSDIGADIGVGL
                     RCYRPLTLPRGGRSMDAIQTLIEALVVAFATTVLAWMVGGLRRETRESIGRLRGEVGL
                     FRDDLAAT"
     gene            2105..2374
                     /locus_tag="VE646_04560"
     CDS             2105..2374
                     /locus_tag="VE646_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79297.1"
                     /translation="MTTLKAELRGEMGRVREDLHGEMGLREDLRVEMGELRDELRGET
                     GELRGEFRTEQREGFSGVREELLILRSDLTQVALAVGARPRAGNE"
     gene            2450..2653
                     /locus_tag="VE646_04565"
     CDS             2450..2653
                     /locus_tag="VE646_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79298.1"
                     /translation="MFWALFALGVVLFLALVVVVLGIVVKGANDGGSQYLLGTRHAGS
                     HPGLTSSDYSEGRDEPDEEQGGT"
     gene            2781..3572
                     /locus_tag="VE646_04570"
     CDS             2781..3572
                     /locus_tag="VE646_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZD79299.1"
                     /translation="MASGGSRTRLRLGDVEVARVGLGTNQLTNTPERVAFIKEAVASG
                     LNHIDTAHTYTDGESEQTIGAAFSPKPDGCILASKGGYAPGHGRPEILRAQIDESLRR
                     LRTEAIDLYYLHRVDPETPFEDSVGALAEARAAGKVRHIGLSKVDVDQIERARAIAPI
                     AAVQNHYNLSERGSDDVIDHCAREGIVFVPYYPLEGGDERALEEIAEGHDTTPMQIRL
                     AWLLHRSPMTLPIPGTLSLEHLRQNLAALDIELTGDEFEALSGGS"
     gene            3770..4186
                     /locus_tag="VE646_04575"
     CDS             3770..4186
                     /locus_tag="VE646_04575"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZD79300.1"
                     /translation="MRSNRFTFKASGDDAGGGFAAVETVLHPVAAAPAHVHERTDEAF
                     YVIGGEIVVEVGEQRLRGTPGSFGFLPRGIPHRYLPQAPGPVRVLWLLSPAGFEGFWR
                     EVGTPVIDGEEPPSPSPPDQVRMAELGRKYLTDFTG"
     gene            complement(4447..4923)
                     /locus_tag="VE646_04580"
     CDS             complement(4447..4923)
                     /locus_tag="VE646_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZD79301.1"
                     /translation="MQPIKDAASAFLEHRRIAVTGVSRNPKGHGSNVVYQRLRAREYE
                     VFAVNPNADEVEGDPCYHDLKSIPGGVQGVVIGTRPELAEDTMRECVDLGITDVWMHR
                     GPGGGSVSEAATEYGRSHGVTVIDGGCPCMFGPTADVGHKVMRFMFTATGNVPRRV"
     gene            complement(5054..5431)
                     /locus_tag="VE646_04585"
     CDS             complement(5054..5431)
                     /locus_tag="VE646_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010474397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZD79302.1"
                     /translation="MTGGLGGLDAITFDCGDPLRLARFWGAVFGADVAAVDGDEPHYV
                     DLVPVPGLPTLRFQRVPEPKTTKNRLHLDVSVEDLEEACGRVEALGGRRISARPYVEY
                     GYEWLVMADLEGNEFCLVIDREA"
     gene            complement(5428..6816)
                     /locus_tag="VE646_04590"
     CDS             complement(5428..6816)
                     /locus_tag="VE646_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZD79303.1"
                     /translation="MADDGRHQDLAEAEAEGAVPRLSVSPPGPRSRELIDRQRRVAWS
                     GALDTMPIGIRRKHGSVIEDVDGNLLIDMLTGWGSTNIGATHPEVLAAAIDALRDYGQ
                     EIPDYVSAPLVVELAEKLVRIAPDPLARVSFEISGTEAVESAMKFMRARTERPFILTF
                     LGQYHGESYGAQAVGSQASGYSRFMRELNNGYVHVPYPHPYRCPFHARPQDCDGICVV
                     DYVREQILPYLVAPDRIAGVMIEPIAGEAGVFIPPDPFWPALMELCGEHGWLWCADEA
                     QTLFGRCGPMFATELWDLHPDLMVLAKALSGGILPIAACLGSEDVMGETEVYAGGTYP
                     WFPPSCAAALKGIEVLERGDLLEHGRALEAIALDRLGPLADRYGIVGEARVKGLYIGV
                     EFVRDRETKEKAVQEARAVHFKCLERGIVGIYDRGMHVVRWQPALSMPEAMFERACDI
                     LEGSIAEVEAGR"
     gene            complement(6824..8437)
                     /locus_tag="VE646_04595"
     CDS             complement(6824..8437)
                     /locus_tag="VE646_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZD79304.1"
                     /translation="MAANTYDAIVIGGGHNGLVAAGYLAKKGARVVVLEARHKTGGAA
                     DTSTPFPEHPDVRVTTLSYVMSLMPPTIVRDLRLESFGYRVFPMGSTYAPQPGGGAIR
                     LVDEEGELRERLAMFSKRDADAVGEWRAWIERSAALLGPVLMETPPKVGSMAIGDVID
                     QVRFVLRHRRGMSVRQAADVTKLFTMSAADLLSEWFESEEVMGALSTDGIIGTWAGPE
                     EPGTAYVLMHHSVGDLGDGQISAWGYPEGGMGAVSLALQRSAESFGADIRVNAPVERV
                     LVRDGRAVGAVIEGGEQVHAPLLVTACHPRIAFLRHLDRHELPDAFVRDIESWKTRSG
                     TVKINLALSRLPEFRDDPGEDPQIRGASIMIAPSIRYLERAFEEARDGRASTAAFSET
                     CVPSVFDRTLAPEGMHVMSMFTQWVPHTWADERHQDELEAYADRMIDIHEDFIPGLKE
                     AIVGRQVIGPWQMEHEWGLIGGNIFHGELVPNQLFHMRPAPGYADYRTPIRGLYQASS
                     ATHAGGGVTGMPGHHAVRQIVRDRKLRRG"
     gene            8563..9027
                     /locus_tag="VE646_04600"
     CDS             8563..9027
                     /locus_tag="VE646_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZD79305.1"
                     /translation="MPPLIDELDLRILDLLQTDGRMPFVKIAEQLGVSDTTVRTRVDR
                     LVRRFGVKFVVDVDPNDLGLVYVYLAVGVRGPALARAVERMTALPSVIFLARTVGTHD
                     LMAEAICRDHEDLMRLLDEIRAIPGVVRMDTLTVLRVEKEDWRFVGLAAGQG"
     gene            complement(9040..>9672)
                     /locus_tag="VE646_04605"
     CDS             complement(9040..>9672)
                     /locus_tag="VE646_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HZD79306.1"
                     /translation="ADIAELKWFHLSPEPVTLAGVRGLLSRTGFTGELGYEFFLLDPD
                     GAEGLWDAVLDAGAAPFGLDAVELLRVEAGLLIQHEDYVPGQTDPYELSLDPYIDLEG
                     HDFVGKQAAARRAVGPSRRMVTLVIQGEEVPEAGTPVTVEGRAVGDVRSPARTPRFGA
                     IALAVLDARCADDGGVVQVDGRPATVRPAPIDDPRKLRPRSDPRDPVRVS"
BASE COUNT         1397 a         3492 c         3344 g         1439 t
ORIGIN      
        1 ccccctcgaa ggggaccggc acggcacccg gcgtgggacc atcggcgacg ggcggagcct
       61 cggcgacggg cgggagtcag gcggcccgcc aaccacggca cctgcccgcc ccacccgaca
      121 tccggcccat cgcggcgaac cccctgcccc acgaggggct ctggcagacc gtgggacgga
      181 cggcggacgg catccccaca ctgcgcgtgg ccttcctgcg ccccgacccc gtccacacca
      241 gcctcgtggc cggcgtggcc tggatggaca cgacgctgct gcgcgcggcc ctggtggccg
      301 gcacccaggt ccccggtggc tcggcctggc agcagggctg gggcgccgag gtcccccgca
      361 gccggcgcgg ctcgctggtg gccacgttca actccgggtt cctgctgaag gactcgcacg
      421 ggggctacta cgcggacggt cggacctacg cgccgctcgt cccgggcggc gcctccgtgg
      481 tggtggaccg ggacggagcg atcacggtcg gacgttgggg ggtggacgtc cagatgggtc
      541 cgcaggtggc gttcgtgcgg cagaacctgc agctgatcgt gaaccacggc cggccggtcc
      601 ccgggctggc caccgactcc taccagcggt ggggagcgac cctcgggaac gcggtgatgg
      661 tctggcgctc agggctcggc gtgaccacgg acggggccct ggtgtatgcg gccggccccg
      721 ggctgtcggt gcagtcgctg gcggcggtgc tggcccgcgc gggagcggtc cgagccatgg
      781 agctggacat caacaccgac tgggtctcgt tcaactacta cacccagaac cggtcctcga
      841 gctggggcgt ggctgcgcac aagctgctct cgacgatgat ccgatcgcag gaccggtacc
      901 tggtcccgga cgagcgcgac ttcttcgcgc tgtcgctgcg tccgcggttc gcgaagaccg
      961 gatagcccgg ggcgggtcag gcgggcgggc cggcggcgat ccggtcccgc acctgctgca
     1021 tccgctcggg gacgtgatgg ggcatctccc agttggtctc cagccaccag gtgcatccgg
     1081 cctcggccca ggggccgacc acttcgccgg ccagggccgg atcctcgggc gtctcgccgt
     1141 cggcgatgag gtcgaggtcg gaggcgccgt gctcgctcaa ccacgcccgc acctcccgtg
     1201 cccgggccgg ggtcccctcg tgatccgagt actgcggcac gatgccgtcg caccgcagca
     1261 cgcgcgccat ggacctcggg cgcggccaca ccccgacgac ccagatcggg atccggtgct
     1321 gcaccggcct gccgatctcg atctggtcgt ccatcgcgca ctcgtaccgg taatgaggtc
     1381 cctcgtgatg cccctggcca gaccacagcg tccgcagcag gtcgatgcct tcgtccatca
     1441 gctccgcccg ctcgcggacg ccggtcacct cgccggtcaa gggaaggtcg tcggtcaccg
     1501 ctccgatccc cacggtcagg atcgtccgtc cctcggacag ctggtcgacg gtggccgcct
     1561 ggctcgcgag cttccagggc cggcgccacg gcagcggcgt cagcagcgtc ccgagcttga
     1621 tcgtccgggt ccggactgcc atggccgcca ggagcgacca cgcgtcgatg ccgtaggcgg
     1681 cctcccacac gaagatgcca tcccagccgg cgtcctcggc catcatcgcc tgctccaact
     1741 gctcggatgc cgagccgccg ggaaggatga agcccttgtt catcggcgcg gctcctcttc
     1801 gccatgatgt gaccaccagt accggctccc ctgctcacgg tagccgctcg cggaccgtcg
     1861 cgcgcgaccg ccggagcgac atcggtgctg acatcggtgt cgggctacgc tgctatcgtc
     1921 cgctcaccct cccccgggga ggacgatcca tggatgcgat ccaaacgctg atcgaggcgc
     1981 tggtcgtcgc gttcgcaacg acggtgctcg cgtggatggt gggcgggctc cgccgagaga
     2041 cccgtgagtc gatcggccgg ctgcgtggag aggtgggtct gtttcgcgac gacctggcag
     2101 cgacatgacg acgctgaagg ctgagctccg tggtgagatg ggtcgggtgc gcgaggacct
     2161 gcacggagag atgggcctgc gcgaggacct gcgcgtagag atgggcgagc tgcgcgacga
     2221 gctccgcgga gagacgggcg agctgcgcgg agagttcagg acggagcagc gcgaggggtt
     2281 cagcggggtt cgggaggagc tgctgatcct gcggtcggac ctcacgcagg tggccttggc
     2341 cgtcggggcc cgccctcgag ccggcaacga gtgatcgatg gaccggtggc gtcacctgaa
     2401 ggcgccgttc ccccttcggg aaccggcacc gaggtagact cgatcgacca tgttctgggc
     2461 gctgttcgcc ctgggggtcg tgctgttcct cgcgttggtg gtcgtcgtcc tcgggatcgt
     2521 ggtgaagggg gcgaatgacg gaggttccca gtacctgctc ggtacccggc acgccggttc
     2581 gcacccgggt ctgacttcgt ccgactactc ggagggccgg gacgagcccg acgaagagca
     2641 gggaggaacc tgaggccctg tggcggacgg cgcaggtcgg ttccctccat cggtagcaga
     2701 ctggtcctca gccgtgccac gcaccacggg ctgcgttgca cggctcttcg gaccacggag
     2761 cggagcgagg aggtcggtgc atggcgtcag gcggctcgag gaccaggctg cggttgggcg
     2821 atgtcgaggt ggcacgggtg gggctcggga cgaatcagct cacgaacacc ccagagcgcg
     2881 tcgccttcat caaggaggcg gtcgcctcgg ggctgaacca catcgacacc gcccacacct
     2941 acaccgatgg tgagagcgag cagacgatcg gtgccgcgtt ctcgccgaag ccggacgggt
     3001 gcatcctcgc gagcaagggg gggtacgcgc cggggcacgg acgtcccgag atcctgcggg
     3061 cgcagatcga cgagagcctc cgtcggctgc ggaccgaggc gatcgacctg tactacctgc
     3121 accgcgtgga cccggagacg cccttcgagg acagcgttgg cgccctcgcc gaggctcgcg
     3181 cggccgggaa ggtccgccac atcgggctgt cgaaggtcga cgtcgaccag atcgagcgtg
     3241 cccgtgccat cgcgccgatc gccgcggtcc agaaccacta caacctctcc gagcgcggat
     3301 cggacgacgt gatcgatcac tgcgcccgcg aggggatcgt gttcgtcccc tactacccgc
     3361 tggagggcgg ggacgagcgc gcgctcgaag agatcgccga gggccacgac accaccccga
     3421 tgcagatccg cctggcgtgg ctcctgcacc ggtccccgat gacgttgccg atccccggta
     3481 cgctgtccct cgagcacctg aggcagaacc tcgcggcgct cgacatcgag ctcacggggg
     3541 acgagttcga agcgctgtcc ggggggtcgt gaccggctcc gcatcgctga tctccaccgg
     3601 cggaagagct cgacggaggg gcgacggcga cctctccaag cccccggctg gcggccgggt
     3661 cgcccgtgcg gcatacttcg ccgtctgccc gcccggacca ggaggctcac gtgagcccag
     3721 cagaggcacg gccgtacctg cgcggcccga cgagggagag gcgctgtggg tgcggagcaa
     3781 ccgcttcacg ttcaaagcca gtggggatga cgcgggcgga gggttcgccg cggtcgagac
     3841 ggtgctgcac cccgtggccg ccgcgccggc gcacgtccat gagcggaccg acgaggcctt
     3901 ctacgtgatc gggggggaga tcgtcgtcga ggtgggtgaa cagcgtctgc gcggaacgcc
     3961 cggctcgttc ggcttcctcc cgaggggcat tccccaccga tacctccccc aggcgccggg
     4021 gccggtgcgg gtgctttggc tcctctcccc cgccgggttc gaggggttct ggcgggaggt
     4081 gggcaccccg gtcatcgacg gcgaggagcc accgtctccg agcccacccg accaggtgcg
     4141 catggcggag ctcgggcgca agtacctcac cgacttcacg gggtgaggat ggtggaaggg
     4201 ccggcccggc gctccggacc ggcccttcac gggtccccgt tgatcggcgg ggagctcacc
     4261 ggccatcacg atagggcgac ctggtctccc catcggtgac caccgacaca gaccggctgt
     4321 gtcgtccgtc acttccctca tacgagggct cccaccgttc ggctcgtacc gggcttgacc
     4381 gatggtcggg tcggcccggc gctcggggac gcgctgtgtc gaagccgatc cgagggggtg
     4441 gacccgtcag accctcctgg gcacgttgcc ggtcgccgtg aacatgaacc gcatcacctt
     4501 gtggcccacg tccgcggtgg gaccgaacat gcacgggcag ccgccatcga tcacggtcac
     4561 gccatggctg cgaccgtact cggtggcggc ttcggacacg ctgcccccgc cgggcccccg
     4621 atgcatccag acgtcggtga tgcccaggtc gacgcactcg cgcatcgtgt cctcggcgag
     4681 ctccggtcgg gtcccgatca cgaccccctg cacgccgccc gggatcgact tcaggtcgtg
     4741 gtaacagggg tcgccttcga cctcatcggc gttcgggttc accgcgaaga cctcgtactc
     4801 ccgagcgcgc agccgctggt acaccacgtt gctgccgtgt cccttggggt tcctggagac
     4861 gcccgtcacc gcgatccgcc ggtgctccag gaacgcgctc gccgcgtcct tgatgggctg
     4921 catcccgacc tcctccccac aggcccaggc cagtgaaagg ccagcctccc ccggcacatc
     4981 cccgatgtcg ggggtcctgc gtcccggcgc gcacgggacc gatggcagcc ggtgcggtgg
     5041 gatccgggag aactcacgcc tcgcggtcga tcacgaggca gaactcgttg ccttcgaggt
     5101 cggccatcac gagccactcg tacccgtact cgacgtacgg tcgtgcggag atccgtcggc
     5161 cgccgagcgc ctcgacgcgg ccgcaggcct cctccagatc ctcgaccgac acgtcgaggt
     5221 ggagtcggtt cttcgtggtc ttcggctccg ggacccgctg gaaccgcagg gtcggcagcc
     5281 cggggacggg gacgagatcg acgtagtgcg gctcgtctcc gtccacagcc gccacgtccg
     5341 ccccgaacac ggctccccag aaacgggcca gcctcagtgg gtccccgcag tcgaacgtga
     5401 tcgcgtcgag ccccccgagc cctcccgtca acgacctgcc tccacctcgg cgatcgatcc
     5461 ctcgaggatg tcgcaggcgc gctcgaacat cgcctccggc atcgagaggg cgggctgcca
     5521 gcgcaccacg tgcatgccgc ggtcgtagat ccccacgatc ccgcgctcca ggcacttgaa
     5581 gtgcactgcc cgcgcctcct gcaccgcctt ctccttcgtc tcccggtccc ggacgaactc
     5641 gaccccgatg tacaggccct tcacccgggc ctcgcccacg atcccgtacc ggtcggccag
     5701 cggaccgagc cgatcgagcg cgatcgcctc caacgcgcgg ccgtgctcga ggaggtcgcc
     5761 gcgttccagc acctcgatcc ccttgagcgc cgccgcgcac gagggcggga accacggata
     5821 cgtcccgccc gcgtagacct cggtctcgcc catcacgtct tccgagccga ggcaggcggc
     5881 gatcggcagg atcccgccgg acagcgcctt ggctagcacc atcaggtccg gatgcaggtc
     5941 ccacagctcg gtggcgaaca tcggtccgca ccggccgaac agcgtctgcg cctcgtcggc
     6001 gcaccacagc cacccgtgct ccccgcagag ctccatcagc gccggccaga acgggtcggg
     6061 cgggatgaac acccccgcct cgccggcgat gggctcgatc atcaccccgg cgatgcggtc
     6121 gggagccacc aggtagggaa ggatctgctc ccgcacgtag tccacgacgc agatcccgtc
     6181 gcagtcctgc ggccgcgcgt ggaacgggca gcggtagggg tgcggatacg gcacgtgcac
     6241 gtagccgttg ttcagctccc gcatgaaccg cgagtagccg ctggcctggg atcccaccgc
     6301 ctgcgcgccg taggactcgc catggtactg ccccaggaag gtgaggatga acggccgctc
     6361 cgtcctggcg cgcatgaact tcatcgcgct ctccacggcc tcggtcccgc tgatctcgaa
     6421 gctcacccgc gcgagggggt cgggagcgat ccggacgagc ttctcggcca gctccacgac
     6481 cagcggcgcg gagacgtagt cggggatctc ctggccgtag tcgcgcagcg cgtcgatcgc
     6541 cgcagccagc acctcggggt gggtggcgcc gatgttggtg gacccccacc cggtcagcat
     6601 gtcgatcagc aggttgccgt cgacgtcctc gatcaccgag ccgtgcttgc gcctgatccc
     6661 gatcggcatc gtgtcgagcg cgccggacca ggccacgcgc cgttgccggt cgatgagctc
     6721 gcgcgaccgc gggcccggtg gggagaccga caggcgcggg acggctccct ccgcctccgc
     6781 ctcggccagg tcctggtggc gtccgtcgtc ggccatcgcg tcgctaccct cgccgcagct
     6841 tgcggtccct gacgatctgg cggaccgcgt ggtggccggg catgccggtc accccgccgc
     6901 ccgcgtgcgt ggcgctggag gcctggtaca gcccccggat cggggtccgg tagtccgcgt
     6961 acccgggcgc cgggcgcatg tggaacagct ggttgggaac gagctcgccg tggaagatgt
     7021 tgccgccgat caggccccac tcgtgctcca tctgccacgg gccgatcacc tgccggccga
     7081 cgatcgcctc cttgaggccg gggatgaagt cctcgtggat gtcgatcatg cggtcggcgt
     7141 aggcctccag ctcgtcctgg tgccgctcgt ccgcccacgt gtgtgggacc cactgcgtga
     7201 acatcgacat cacgtgcatg ccctcgggcg cgagcgtccg gtcgaacacg ctcggcacgc
     7261 acgtctccga gaacgccgcc gtcgaggccc gcccgtccct cgcctcctcg aaggcgcgtt
     7321 cgaggtagcg gatcgagggg gcgatcatga tcgaggcgcc ccggatctgc gggtcctcgc
     7381 ccgggtcgtc gcggaactcc ggcaggcgcg acagcgccag gttgatcttg accgtgccgc
     7441 tccgcgtctt ccagctctcg atgtcgcgca cgaacgcgtc cggcagctcg tgccggtcca
     7501 ggtgccgcag gaaggcgatc cgagggtggc aggcggtcac caggagcggg gcgtggacct
     7561 gctcgccccc ctcgatcacc gcgcccaccg ctcgtccgtc gcggaccagg acccgctcca
     7621 ccggcgcgtt cacgcggatg tcggccccga aggactccgc cgagcgctgg agggccaggc
     7681 tgacggcccc catccctccc tccgggtacc cccacgccga gatctggccg tcgcccaggt
     7741 cgccgaccga gtggtgcatc aggacgtagg ccgtgccggg ctcctcgggg ccggcccagg
     7801 tgccgatgat cccgtccgtc gagagggccc ccatgacctc ctcggactcg aaccactccg
     7861 agagcaggtc ggcggccgac atcgtgaaca gcttggtcac gtcggccgcc tggcgaacgc
     7921 tcatgccccg ccggtgacgg agcacgaacc ggacctgatc gatcacgtct ccgatcgcca
     7981 tggagcccac cttgggcggc gtctccatca ggaccgggcc gaggagcgcc gcgctgcgtt
     8041 cgatccatgc gcgccactcc cccaccgcgt ccgcgtcgcg cttggagaac atggccaggc
     8101 gctcccgaag ttcaccctcc tcgtccacca ggcggatcgc gcccccgccc ggttgcggcg
     8161 cgtaggtgga acccatcggg aagaccctgt aaccgaacga ctcgagccgc aggtcgcgga
     8221 cgatggtagg cggcatcagg ctcatcacgt aggagagcgt ggtcacccgc acgtcggggt
     8281 gctcggggaa cggcgtgctg gtgtcggccg ccccaccggt cttgtgccgg gcttccagca
     8341 ccaccactcg agcgcccttc ttggccagat agccggcggc caccagcccg ttgtgcccgc
     8401 ccccgatcac gatcgcgtcg tacgtgttcg ccgccatggc agccctctcc cgctcggacc
     8461 cggccgacga tagtggtcca caagggagga tccgagagcc ttcgacgccg tcgaccttcg
     8521 atattcgatg gatggacgcc gatctgtggg attatccgaa gcatgccccc gctgatcgac
     8581 gagctcgacc tgcggatcct cgacctgctg cagaccgacg gccggatgcc gttcgtgaag
     8641 atcgccgagc agctcggcgt ctcggacacg accgtcagga cccgggtcga tcgcctggtc
     8701 cgccggttcg gcgtgaagtt cgtggtggac gtcgacccca acgatctcgg cctggtctac
     8761 gtgtacctgg ccgtcggcgt gcggggcccc gccctggcac gcgccgtcga gcggatgacc
     8821 gcgttgccct cggtgatctt cctggcccgc acggtcggca cccacgacct gatggccgag
     8881 gcgatctgcc gggaccacga ggacctgatg cggctcctgg acgagatccg ggcgatcccc
     8941 ggcgtggtgc ggatggacac gctcaccgtc ctccgcgtgg agaaggagga ctggcggttc
     9001 gtggggctcg cggccggcca ggggtgagcc cggccgcgct caggagaccc tgaccgggtc
     9061 gcggggatcg gaccgcggcc tgagcttgcg cgggtcgtcg atcggagcgg gacgcaccgt
     9121 ggccggacgt ccatccacct ggaccacccc cccgtcgtcc gcgcatcggg cgtcgagcac
     9181 ggccagcgcg atcgcgccga aacgaggcgt ccgcgcgggg ctccgcacat cgcccaccgc
     9241 tcggccttcc accgtgaccg gggtccctgc ctccggcacc tcctcaccct ggatcacgag
     9301 ggtgaccatc cgccgcgaag gacccaccgc gcgtcgcgcc gccgcctgct tgccgacgaa
     9361 atcgtggccc tccaggtcga tgtaggggtc cagcgagagc tcgtacggat cggtctggcc
     9421 cgggacgtag tcctcgtgct ggatcagcag cccggcctcg acgcggagca gctccacggc
     9481 gtccagaccg aacggcgcgg ctccggcatc gaggacggcg tcccacagcc cctcggcgcc
     9541 gtctgggtcg aggaggaaga actcgtagcc cagctctccc gtgaaccccg tcctggacag
     9601 caggcctcgg acgccggcca gcgtcacggg ctcgggggat aggtggaacc acttcagctc
     9661 cgcgatgtcc gc
//
LOCUS       DASQQQ010000110         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_173263, whole genome shotgun sequence.
ACCESSION   DASQQQ010000110 DASQQQ010000000
VERSION     DASQQQ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173263"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VE646_04610"
     CDS             <1..581
                     /locus_tag="VE646_04610"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZD79307.1"
                     /translation="PGDRRFSEDDGLRPRSPYAASKAGADLLCSAYHTTYGTDITIVR
                     GTNAYGPRQLERVMPTYTLAALRRLPIPVYGAGQQRREWLHVEDWVRAAALILERGEP
                     GVVYNLGDGFELSNLELAERICDLAGAPRSLISFVPDRPGHDFRYGVRCERLRALGWE
                     PGVPFAEGLARTVDWYRTHLDEVVRATPAPVR"
     gene            607..1437
                     /locus_tag="VE646_04615"
     CDS             607..1437
                     /locus_tag="VE646_04615"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZD79308.1"
                     /translation="MRIAVTGAEGGLGRAFLAQVPGHHDVLAFGHAELDVGDHHAVMR
                     TLVPLRPELILNLAAFTRVDACESDPDRAYRDNALGPQSVALAARACEALVLHVSTDY
                     VFDGEKGAPYDELDEPRPLSVYARSKLAGERFVRQVLPSHFVVRTGFVFGGGSDFLSG
                     ALRRLAAGEPAGGLADRVGSPTCVRELASRLLPLALTGRFGTYHLAGPEATTWFDVLQ
                     RAKRIGDLPGEVRPQRAEELALPAPRPRSTALTSVFAPAVGIEPMPPLDRSLSDLLGS
                     "
     gene            2054..>2248
                     /locus_tag="VE646_04620"
     CDS             2054..>2248
                     /locus_tag="VE646_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79309.1"
                     /translation="MQAAWSENVSAGRIRRRGDLEARRSREQAERVQSCRELCLSYEE
                     EGTTGELDGRLPAAEMAVVQA"
BASE COUNT          311 a          799 c          810 g          328 t
ORIGIN      
        1 aacccggcga ccggcggttc agcgaggacg acgggctgcg cccccggagc ccctacgcgg
       61 ccagcaaggc cggggccgac ctgctgtgca gcgcctacca caccacgtac gggaccgaca
      121 tcacgatcgt gcgtgggacc aacgcgtacg gtccacgtca gctcgagcgc gtgatgccca
      181 cctacacctt ggctgccctg cgccgcctgc cgatccccgt gtacggggcg gggcagcagc
      241 ggcgcgaatg gctccacgtc gaagactggg tccgggccgc cgccctgatc ctcgagcggg
      301 gggagcctgg ggtcgtctac aacctcgggg acgggttcga gctctcgaac ctcgagctgg
      361 cggagcggat ctgcgacctg gccggggcgc cgcggtcgtt gatctcgttc gtgccggacc
      421 ggcccggcca cgacttccgc tacggggtgc gatgcgagcg cctgcgggcc ctgggctggg
      481 agccgggggt gccgttcgcg gagggactgg cccggaccgt ggattggtac cggacgcacc
      541 tggacgaggt cgtgcgcgcc acgcccgcgc ccgtgcggtg agtggtcgcc cgatgaggat
      601 cgtccgatga ggatcgcggt cactggcgcc gagggcgggc tgggtcgcgc cttcctcgcc
      661 caggtccccg gccaccacga cgtccttgcc ttcggtcacg ccgagctgga cgtgggcgac
      721 caccacgcgg tgatgcgcac cctggtgccg cttcgccccg agctgatcct gaacctggcc
      781 gccttcaccc gggtggacgc gtgcgagtcc gatcccgacc gcgcctaccg ggacaacgcc
      841 ctcggtccgc agagcgtggc gctcgcggct cgggcctgcg aggcgttggt gctgcacgtg
      901 tccacggact acgtcttcga cggcgagaag ggggcgccct acgacgagct ggacgagccc
      961 cgtccgctct cggtgtacgc gcggagcaag ctggcggggg agcggttcgt ccggcaggtg
     1021 ctgccctcgc acttcgtggt tcggaccggc ttcgtcttcg gcggcgggag cgacttcctg
     1081 agcggcgcgc ttcggcgtct ggccgcgggc gagccggccg gcgggctggc ggaccgggtt
     1141 ggctcgccga cctgcgtgcg cgagctggcc tcgaggctgc tgccgctggc cctcaccggg
     1201 aggttcggga cgtaccacct ggccggtccc gaggccacca cctggttcga cgtgctgcag
     1261 cgggccaagc ggatcggcga cctgccgggc gaggttcggc cccaacgggc cgaggagctc
     1321 gcgctgccgg cgccccgccc ccggtccacc gcgctcacca gcgtgttcgc gcccgccgtg
     1381 ggcatcgagc cgatgcctcc actggaccgg tcgctctcgg acctattggg gtcctgaggg
     1441 gtcgttcgcg tccccggtcc gtgtggtggg cgcgtccccg ggtctttcct gcctccggcc
     1501 gtcgatggca tacgcaccgt tgtggctgcg gagccaccct caccgatagg tcggccccct
     1561 cgccaccacc cctcgaaggg ctcgggcggc cgtcagactg cgatcggcaa cggggcatcg
     1621 gacggccaac tccgcacgga aaagttggct cgcccagggg catgtcgctc aagcggtgga
     1681 gcggtgtggg agcggtctcc tagaggctgg ctgatcccat ccaggagcct ggccttgtgg
     1741 tggcctagcc tcggtcacca cggctccacc ggatccagaa gagcgtcttc cccgctgatg
     1801 tatccaggtc cgcgggaggg atctgccagc gccgagtcga ccttacgatg tagaagacca
     1861 ccgctcccga gagccgccac cacgacgccg agcccaaccc aggaccacca gaccatcaac
     1921 gcaagcgtac gcctcggccc cacgagaagc gacggccctc gccgagctgg gggatccatc
     1981 ctggtccaga tccctccccg cggagtgacc taggtcacag acaggtccaa acacatgttc
     2041 gatactgatg gacatgcagg ccgcctggtc cgagaacgtc tccgcgggaa ggatccggcg
     2101 ccgaggcgac ctggaggccc gccggagccg ggaacaggcc gagcgggtcc agtcctgccg
     2161 ggagctctgc ctcagctacg aggaggaggg caccaccggg gagctggacg ggcgccttcc
     2221 ggccgcggag atggcggtgg tgcaggcc
//
LOCUS       DASQQQ010000111         3286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_117594, whole genome shotgun sequence.
ACCESSION   DASQQQ010000111 DASQQQ010000000
VERSION     DASQQQ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117594"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VE646_04625"
     CDS             <1..1149
                     /locus_tag="VE646_04625"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HZD79310.1"
                     /translation="GAAGAFATPLLLGEAGGATALSLYIVGVTGWTGVLAFFHGWRPL
                     FVTTLAGAFGLLAMGSAQAPPGGRLWLAVAGAWSWAVVTVLPLLRERLPGGRIHRGRR
                     PVPVFLRTRVARPEQLDRLLVAGLIPVGTVLLVILQATLWDLERPGAAAIGFAATAAL
                     AALLVGPGPMGARARIAGVAPALLTYLGSALLVPDDALRVALLATGGAATFLIPSVRA
                     APGAEPLGHVVFAILAAWITAELSDAARPIGFLTLLAAAELAAIAAAVAVALRVLAPP
                     ARLTYLIAAHLAVLVWVLLCARGVDGGLAWVSILWGVYGAALLVLAVGRSLAPVRIAA
                     LATLGLVAAKLLLVDTAGVGAGWRIILFIGFGAAFLGLGYISNPEPRS"
     gene            complement(1229..1663)
                     /locus_tag="VE646_04630"
     CDS             complement(1229..1663)
                     /locus_tag="VE646_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79311.1"
                     /translation="MPAHRLRLTLRYIEILDSKDVDMYGEFRFRFQVSVPSRQLSSET
                     RIPQEGHLSIADHPAMNKVSRRDVLFEGEVEDGDTLVVEASGEELDRLSANDQLTSYR
                     REFSGPVDGWLGEHGPWDEGGDNVKDPEQLGDWRLAYAIEAV"
     gene            3091..3162
                     /locus_tag="VE646_04635"
     tRNA            3091..3162
                     /locus_tag="VE646_04635"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3123..3125,aa:Thr,seq:ggt)
BASE COUNT          407 a         1195 c         1162 g          522 t
ORIGIN      
        1 ggagcggcgg gcgcgttcgc cacgccgctc ctcctgggtg aggccggcgg cgccacggcg
       61 ctgtccctct acatcgtggg ggtgaccggg tggacgggcg tcctggcgtt cttccacggc
      121 tggcgaccgc tcttcgtgac caccctggcc ggtgcgttcg ggcttctcgc catgggctcc
      181 gcccaggcac cacccggtgg caggctctgg ctcgccgtgg ccggcgcgtg gtcgtgggcc
      241 gtggtcaccg tgcttccgct cctgagggag cggctcccgg gcgggcgcat ccatcgcggg
      301 cgacgccccg tgccggtgtt cctccggacc cgggtcgccc ggcccgagca gctcgatcgg
      361 ctcctggtcg ccgggctgat ccccgtgggg accgtgctcc tggtcatcct ccaggcgacc
      421 ctctgggacc tcgagcggcc gggggcggcg gccatcgggt tcgccgccac ggccgcgctc
      481 gcggcactcc tggtcggtcc gggtcccatg ggcgcccggg cccgcatcgc cggggtggcg
      541 cccgccctcc tcacctacct cgggtcggcc ctcctcgtcc cggacgatgc cctccgggtc
      601 gcgcttctcg cgaccggagg cgccgcgacc ttcctgatcc cgtcggtgcg cgccgccccc
      661 ggcgcggagc ccctgggcca tgtggtcttc gcgatcctgg ctgcctggat cacggccgag
      721 ctctcggacg ccgcgcggcc gatcgggttc ctcacgctcc tcgccgccgc ggagctcgcc
      781 gcgatcgcgg ccgccgtggc ggtagcgctg cgagtgctgg cgccaccggc ccggctcacg
      841 tacctgatcg ccgcacacct cgccgtgctc gtctgggtgc tgctgtgcgc gcggggggtg
      901 gatggaggac tcgcctgggt cagcattctc tggggagtct acggcgccgc cctcctcgtc
      961 ctcgccgtcg gcaggagcct cgcacccgtc cgcatcgccg ccctcgccac gctcgggctg
     1021 gtcgccgcca agctactcct cgtggacacc gccggtgtgg gcgccggctg gcgcatcatc
     1081 ctgttcatcg gcttcggcgc cgccttcctg ggtctcggct acatttccaa tcccgagccc
     1141 cgctcgtaga ccgcgcccgc cgggatgaaa gaggcggggc cgccccccgg gacggccccg
     1201 cgccaccacc ccgcccgcgt tcggtcgcct acaccgcctc gatcgcgtac gccagccgcc
     1261 agtccccgag ctgctccggg tccttcacgt tgtcgccgcc ctcatcccac gggccgtgct
     1321 cgcccagcca gccgtccacg gggccactga actcccgacg gtacgacgtc agttgatcgt
     1381 tcgcgctcag ccggtccagc tcctccccgc tggcttccac caccagcgtg tcgccatcct
     1441 cgacctcgcc ctcgaacagg acgtctcgcc gggagacctt gttcatcgcc gggtggtccg
     1501 cgatggacag gtggccctcc tgcgggatcc gcgtctccga ggagagctgt cggctcggca
     1561 cgctcacctg gaagcggaag cggaactcgc cgtacatgtc cacgtccttg ctgtccagga
     1621 tctcgatgta ccggagcgtt aggcggaggc gatgcgcagg catttcatga cctcgttctg
     1681 gttggacgtg gatgtcccgg ggcggcctgt cgtccgggaa gacgcactgt gacagcggaa
     1741 aggtgtcggt ccggtccgga ggaggcaagg gtgggggcgg gatccgaacg ggctgcgggg
     1801 gaggcgccgc gctcgcgccg cgcagccgct tctcgatgtg acgccgcagc gccacccggc
     1861 gcggccgggg agggtgcgga gagcccgccg gcgagggcgg gtcctcggct cggtccgttc
     1921 cgagccggcg gtcgcctcct cgacccaacc gggtcccgga agaacgcggc cccgtgccat
     1981 tggcagtcct cgcgcccggg ggtcccggcg acggcgcgga cgaccagggc gacgattcgt
     2041 gagttcgcgc ccgcgctccc tgcgcgcgag ggcgcgcgcg cttgctatgt gcgctctgct
     2101 ctgatctctc cggcggactc gagtgtgggc gagcgggccc tgtccggcgg cagatccagc
     2161 ggtgtgagcc ggtgaagtcc gcgtcgagcc cccgctcgta ctccagccgg agcctgggga
     2221 gctccaccac catcacgcgg ccctcttccg acgctcgacc ccacgggcgg tcccggtgtg
     2281 gaagcgtgga gcttcccgca gacgagtctc cactccgtct gctccgatcc gctccgcgtt
     2341 ctccgtgtca cgtggctccg aaagttgggt ggcccccgga gtaggagaat cgtttgatga
     2401 gcccgagaaa ccggagccac cgaccccggt cgccctggca gtctgaagaa atggggaaat
     2461 tccaccctgt gaaggacctt ccccggagat cgtcgctgat ctgtcgtaac ctgttgaacc
     2521 gcaacagcgg gtatgtggga agtggtcgac ggcgtgacac ggaggcgtcg gagggctccg
     2581 aggggtcgga ggacagcccc tttgcatggc tcgcctccaa ccgccggctg ggagggcgtg
     2641 gacgttgaac ttgaagcgca cgcccacgcg caggtccgcc agtcgcaggt aggacagccc
     2701 ctgcgagaca tgcacggcga cgtggcggcg tggaaccggc actccgacgc cgtccgagcg
     2761 ctccgtgtca cgcggttccg gaggtggggt cgcccccggg ggcaggcgaa tcgccggatc
     2821 ggccaggaaa ccgagtcgcc ggcccgggtc gccctggcaa gtctgaagaa atggggaaat
     2881 tccaccccgt ggtgttgaca gcgcagagcg ccccccgtat actgaaaatc tgtcttttcg
     2941 agcgggggat cccgctccgc cgtcgccggc ccgaatcggc gcggcacacg gacgcgccgc
     3001 acggcttcgg cccgcgacgc gtccgtgcca cgaaaggcag ggacacgagg gcggcccgca
     3061 ccggacccgg tgcccgtcgc aggctggaat gctcgcgtag ctcaggggta gagcgcttcc
     3121 ttggtaagga agaggtcccg ggttcaaatc ccggcgcgag ctcgttggct ggcggtccat
     3181 gcgagccggc gccggggccg ggtgcccggg cgccggccgc tgatcgctta caggggctgg
     3241 gtttttaccg caccgtcaca ggacgtcgag ggcaaggaaa tgggca
//
LOCUS       DASQQQ010000112        19576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_391249, whole genome shotgun sequence.
ACCESSION   DASQQQ010000112 DASQQQ010000000
VERSION     DASQQQ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 19576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19576
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391249"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VE646_04640"
     CDS             complement(<1..931)
                     /locus_tag="VE646_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79312.1"
                     /translation="MRRAILSIIFVLAALATTALPEPVAEAAYPATNGLLAFRGDRAN
                     GQGDLFTLDPASRQEVALTNTADMQEMDPAWSPEGTRIAFTRRPKLQARPDLFVMNAN
                     GRGRVRLTATPVAERHPAWSPDGTTLVYAARTSARGPTRLFRIRADGRGRVRLTFESS
                     GSYDDDPAWSPDGSRIAFSTNRDGGFPELYLMDPDGSHPLRLTSNHLLDADPSWSPDG
                     TRLAFVRCSEDGTSEIYLMNADGTGQIDLTNTPDLQESDPAWSPDGAAIAYAAVPVGG
                     GEPQIHIMNADGSGQAALTSGPGGAFSPSWQPRP"
     gene            957..2147
                     /locus_tag="VE646_04645"
     CDS             957..2147
                     /locus_tag="VE646_04645"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HZD79313.1"
                     /translation="MNATTSRRLVLGLAVTIAVAGLSPAAGAGPSRDRDRSEAPLRKV
                     IRRHIAPGLIFTKIVQKKIPLRTYILRADVRTTPLTMDVALGGSAMPQRDRTSRIANR
                     HGAIAAVNGDFGTFSGGPTHPFEEDGTLVRSSISPGFMFAVSKDEQQMFVQRPVQWMS
                     ATLPSGLVWPIARWNDGPPGTGEITANTPVGGSLEPPPPGACSARLLPSGSPEPSSTG
                     DGMQQSFTVDVTGCFEDGLARNGGVVLSAIPASDEALQLLSLAPGTPVMLRWTFGWTN
                     VYDAMGGVPQLVADGRNVAGSCSGSICNRNPRTGVGITADGRVIMVVVDGRQSKWSVG
                     LTMNQFGRLMVRLGAVSALNLDGGGSSTMVIRGKVVNRPSAGFERRVTNALLVLPGPD
                     FDEP"
     gene            complement(2394..4232)
                     /locus_tag="VE646_04650"
     CDS             complement(2394..4232)
                     /locus_tag="VE646_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HZD79314.1"
                     /translation="MAAAVVTAGSLGAFQALALIGAPAASAVSTCAFNPLTHTVAVTM
                     DSGDTVTMTVDGTTGAIELGGSACGSATISNTTSIVVLGTPSTDETLVIDNNHDVSFP
                     ASIAWAIDLGTGTGDALEIHLEDGVDGSVTVGDSAFTMNGAPGQMFGVEDVNVYGGDG
                     DDTIDGSALTATGPSFSAGGGDGDDTITGGAGDDSLYGDAGDDWLAGGAGDDEVHGNA
                     GADTVYEGAAVNGADVLSGGSNAGDTIDYGDRVTDVALVAGGGAVSGENLDAATDCEA
                     GETDEEMDDVDGFDTYITGSGDDCIVGTVADETFVPGEGDDSVDGKGGTDTLDYSSSL
                     AGVTIDPAAGTATGQGDDEFADIEEFVGSDFNDTMIWDGSSVFYGGDGRDTVDASAMT
                     TGVTIDLSGGALFPGQDVENAIGGSGDDTITGNDFRNSLWGGPGHDMIDGGLSNDTLN
                     GGPGPDVLDGEEGADLVSYRSETGGVTVNLALGTGSAPSGDDALSNIESVIGSPFNDN
                     ITGSEDSNLIKALRGRDSVRAGGGPDTVRLGPGNDSARGGSGDDNLFGGNGNDRLFGG
                     SGDDLGAGGRGKDICKSIEIKRSCGTAKHPKRALGTAAAKLLARLG"
     gene            complement(4583..6196)
                     /locus_tag="VE646_04655"
     CDS             complement(4583..6196)
                     /locus_tag="VE646_04655"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZD79315.1"
                     /translation="MAIGVSELGNGSYRDLDDRGLVLDFQAGEAEAYQEIFRRYRALA
                     WSIAFRLLASVDDAEEATQETMLRVYQGLPKFNGRYQLQAWVARIATNVSLDKLRARA
                     RRPQEGVAARDRIEDLQVDGGQDPFELVERVLERERVLAVLEELPDHHRRALVLREVE
                     GRSHQEIGEALEITAAQAKALIHRAKGSFRRAWQGNGNGNGRRGVSALVPWVLFPPRL
                     PHLLRRLIGTLGESGGGQVASQAATQATTAATAAPAVTAAAASGAERLTAAAMAFLVA
                     GSVTAGVVVARTPKAEEAKPWTSPAAVQVVTPVSAPAIRPNQVVLVETKQQHHHKKKH
                     HQQKASEPAAVESPSMDVDPADEPSGAPEPEPTESPSAEPTTAPIGPAPDWDMTFQAG
                     IPWAGSCADCPAATWLTSSKVKGTAGDTVSIAQVAVGSALDSQGQPSWKAYVQYAGSA
                     EGTTGRLDFQFKLATDLGWYSYSGIAVLSSGGATSDGGYDYTFVGPYHLDDAPGEEAV
                     PVQGSVQFKVRFWADGTSLYATDVSLFEA"
     gene            6599..7108
                     /locus_tag="VE646_04660"
     CDS             6599..7108
                     /locus_tag="VE646_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HZD79316.1"
                     /translation="MRIVPLTLHADGRGSLTEIFRRSWVPGAREVVQVNLSISDSGVL
                     RGLHFHRRQADYWCVLSGRALVGLFDLREGSPTRMHKAEVPLDAELERVGLYLPPGIA
                     HGFYAQTELVLAYLVDGYFTGEDELGLAWDDPEVAIAWPTDAPILSERDRSNPSLAQA
                     LAHAPTFVG"
     gene            complement(7234..7527)
                     /locus_tag="VE646_04665"
     CDS             complement(7234..7527)
                     /locus_tag="VE646_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79317.1"
                     /translation="MGPREVNVPREVGHRLQDISEASARLHERLAELQHWAWNEQGIA
                     QLPAGRSMEALFERQQEMLKAIQDLAVALDTWISLSQSLTEHYLPRLVRLLIT"
     gene            complement(8242..8820)
                     /locus_tag="VE646_04670"
     CDS             complement(8242..8820)
                     /locus_tag="VE646_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZD79318.1"
                     /translation="MATRSIPGISASRDVVHTGQVDDRAASGQASPLVARARQGDRVA
                     FALLYARYHPALYRVARFHVPDGPEDVVAETFVRAWRDLPRYRDAGTPFAAWLYGIAL
                     HVLDELAGSRRSQPDGEPVQEPEGSERDERVSLAEAMGHLSSEERQVLELKFLIGLSS
                     AEAATAIGRTADAVDSVQWRALRSLRAMLEER"
     gene            9039..10130
                     /locus_tag="VE646_04675"
     CDS             9039..10130
                     /locus_tag="VE646_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZD79319.1"
                     /translation="MEGTRLSRRVLAALIVGTLMLALVPGLSDVAARAQTAPSHPQPQ
                     LDRQLQQARKDLRKTRHRIRDELRRLRKMTKQMDHLATRISKNRSALDRTKAHMEKLK
                     KEIARVKARLAYLEALLAERSRQAYILGPGTPLMYLLTASSAADAASRIVMLDEMNRR
                     DAVLADQVDQARADLGFRRESLARVKFARRILAYALKDAQKQLAAKMAKTQRLWKQLK
                     AHKVLVLDRISRLHPFAMCPVQGPVAIADDFGIWVHRPKNWGGDHIHQGNDMMAAMGT
                     PIVAPFDGVAVDATNHIGGTAVKVIGKYGYVYNAHLSRFGQLGPVTKGTVVGYVGATG
                     NAGGPHDHFEWHPGGGDAVDPHPFLMQVC"
     gene            10278..10706
                     /locus_tag="VE646_04680"
     CDS             10278..10706
                     /locus_tag="VE646_04680"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020511114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZD79320.1"
                     /translation="MPVREAEAEWTGTLQEGDGTMRFGGGAFEGRYSAASRFEEGPGT
                     NPEELIGAAHAGCFSMALAGALVRAGFGPEAIRTGARVHIEKGDPGWAITKIDLRTEA
                     TVPDIEDARFHEIAEEAKRDCPVSKALAAVPIELVATLAS"
     gene            complement(10712..13933)
                     /locus_tag="VE646_04685"
     CDS             complement(10712..13933)
                     /locus_tag="VE646_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZD79321.1"
                     /translation="MPVPCPACGRENPVGARFCMWCASPLPAEEPAPPGSRKSVTVLF
                     CDVVGSTSLGEQLDPESVREVMRRFFGRMGTILEHHGGTVEKYIGDAVMAVFGVPTVH
                     EDDALRAVRAASEMHRALPSLNEDLRAGWSVSLDTRIGINTGEVVVGDVAAGQALVVG
                     DAVNVAARLEQAASPGQVLLGRDTYRLVRGVVGAEPVEPLVLKGKRERVSAYRLLSDL
                     PEGPLLAERGDPPLVDRAAELDALRQAFAAVAEQRSCRLVTVVGSAGVGKSRLVREFA
                     ATLEGRATILQGRCLPYGEGITFWPVAEIVKQACDIHDDDPRAVATGKIGTVLSGAPE
                     ADLVAERVGSIMGLAVAAVDIQETFWAIRRLLEWIGREGPAVVLFDDIQWAEPTFLDL
                     VEYLAGWIRDAPVLLLCAARPDLEDLRPAWGTGVRGDRLSPTRLTEAESEQLLRGILG
                     GGAGDPRATRRITEAAGGNPLFLEELLRMLEDDGMVRREDEAWTAVDLSHISVPPTIQ
                     ALVSARLDRLTPDERTVIRCASVIGKVFWWGAVSHMLEARVRRDVGGHLQALVRKDLI
                     RPGRSTFAGQDAFRFHHLLVQEAAYRSTPKGVRAELHERFAGWLTATAGDRRVEFEEV
                     LGYHLERAYLYRAELGAVGEAEHQLAGRAAGHLAAAGRRAQARGDLPAAADLLDRAVS
                     LLPAGDAGRRALLPDLGEALMETGDLTRADGLLAAAIEEAEAAGDRGVAAHAAVVRLL
                     LEESTEPEERSSHALRQLEAVIPVFEELGDDLGLARAWRLLGDVEWTRSRYGAVDVAL
                     GRALEHARRAGATWEEAEALGQYTGSGVYGPAPVAEVIERCDQVLASGGRNRSVQARA
                     LRALASLRAMQGRYEEGRDLARQAADLLEDLGLALRAAFVSETLAFVERLAGEHAAAE
                     RELRAGFDAMTELGERGYRTTSAALLAHAILAQGPERVPEAERWLQIAEEAAEDDLST
                     QVLLRSARGRVLAARGEPAAAERSAREAVSLAEGTDDLNMRADVLLDLAEVLRTRGND
                     PDAAAAREEALGLYRRKGNLSSAARTERLLESSKRPAETDGVR"
     gene            14029..14424
                     /locus_tag="VE646_04690"
     CDS             14029..14424
                     /locus_tag="VE646_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79322.1"
                     /translation="MSIEIDQEFSVIGTDDRLSTRFAEASSTSPVVVEQQLYEDLGSD
                     EEWGERVGELRGVCFPVGQGQALCNVVLRFDGKLGKGSVSAAGILDVAGEADLSGAMA
                     ISGGTGPFRQRRGELQVTSWNPKRWRLAV"
     gene            complement(14450..14532)
                     /locus_tag="VE646_04695"
     tRNA            complement(14450..14532)
                     /locus_tag="VE646_04695"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14497..14499),aa:Leu,seq:taa)
     gene            complement(14564..15391)
                     /locus_tag="VE646_04700"
     CDS             complement(14564..15391)
                     /locus_tag="VE646_04700"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZD79323.1"
                     /translation="MSRKSRNRQLAKLAQRRYEERRRQQRKRATIIGVAFGVALIGLA
                     VLGFEFLTGNGPNSAASGTPTPSAAAPGTKTGTVEPSPGPKVVACGAQAPKGATKPKP
                     QFAGPPPMRLDASKTYTATIETSCGTFRVRLLADQAPQTVNSFVFLSREGFYDATRIH
                     RIDTSLDIIQGGDPKGTGTGGPGYSIKDELSGKESYGPGVVAMANAGPNTGGSQFFVV
                     YGDKGHTLDPTPNYTIFGRVVKGLDVVRRIAGVPLQGSSQQPKQAVYLDKVTIDVSG"
     gene            complement(15403..16791)
                     /locus_tag="VE646_04705"
     CDS             complement(15403..16791)
                     /locus_tag="VE646_04705"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HZD79324.1"
                     /translation="MSELLGSDLPAVRDQLGREPVTPFTVVARCPGGHPLVIRNRPFD
                     PRGHPFPTLFWLTCPEAVRALSRLESEGWIGRLNDRAREDQPFARELERAHREYALER
                     TRNDPQGRSSGGVGGARAGIKCLHAHYANHLAGGADPVGAWAAARVEPVHPEERPGRV
                     AAVDLGTNSIRLLVASQASGGGLEAFARDMLVTRIGQDVDRTGRIDPEALRRTVDVLA
                     RYSRRARALHAERIRVAATQAVREAGNREELERAVRELTGAELRILSGREEAALSFLG
                     ATGGLEQPAPYFVLDIGGGSTEFVVGSDRPHAAVSKRIGSVRLTERLVRDDPPSLAEL
                     TSMRQLVGRVLDEVEQDVPVQEARTMVAVAGTATTLRAVAADLTFYDPERIHRTWLSL
                     VEVRAVLDRLRRMSTAERAALPVMAPGRADVIVAGAVVLAEVMHRFGFARALVSETDI
                     LEGLASELLTAR"
     gene            complement(16801..17163)
                     /locus_tag="VE646_04710"
     CDS             complement(16801..17163)
                     /locus_tag="VE646_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006689791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HZD79325.1"
                     /translation="MADLEVGAVIEGTVSRITPYGAFLTLDGGKVGLVHISEIDRNYV
                     RDVHDHLRESDVVQAKVIAIKEDGKIDLSIKALQDPAPQRSRRGADPGFESMLKKFMR
                     QSEERQVDYKRSVEHKRK"
     gene            17329..18282
                     /locus_tag="VE646_04715"
     CDS             17329..18282
                     /locus_tag="VE646_04715"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZD79326.1"
                     /translation="MPGMRRRWLAAVLLAGIVAAGCASGTPGRPGGSIPAPTSASAAS
                     GSDPATIEHVVVIVMENHEYGRIIGSPQAPYLNRLARRNVLLTHEYAIRHPSLPNYLA
                     LTAGSTFKISSDCTSCRVKARNLVDQLEAHGISWRAYMESMPSPCFTGATAGTAPNDY
                     AKKHDPFMYYDDIRKRPRRCRQVVPLRRLARNLEAGTLPRFAWITPNECHDMHSCGVA
                     TGDTWLKRWVPQLLPALGSNGILLVVFDEGSSNAGCCSIPGGGHVAAIIAGPGAGHGI
                     RIRTALDHYSLLRLIEDAWGLRHLRRAGDAVTPTVTGWSSG"
     gene            complement(18288..18617)
                     /locus_tag="VE646_04720"
     CDS             complement(18288..18617)
                     /locus_tag="VE646_04720"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HZD79327.1"
                     /translation="MTVRAEAATGTRVRLTGRAAFLIAAVTLLALLAVGPARAYLSQR
                     GQIRDLQRQVQVLEGSNATLQRQVTRLHDPVELERLARQCLGMVRPGETAFVIGPPTA
                     DGGPADC"
     gene            complement(18614..>19576)
                     /gene="eno"
                     /locus_tag="VE646_04725"
     CDS             complement(18614..>19576)
                     /gene="eno"
                     /locus_tag="VE646_04725"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020572460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZD79328.1"
                     /translation="GANAILGVSLAVAHAGANSVALPLYRYLGGSGTHLLPTPMMNVI
                     NGGVHADNELELQEFMLVPVGAASFSEALRWCAETYHALKSLLKGRGLATGVGDEGGF
                     APQIGTAAEALSVMVEAIGSAGLEAGDEMALAMDPAASEIYRDDTYRLEGSDRSSADM
                     VGFYRALLDGFPLVSIEDGLAEDDWEGWGHLTRELGGRCQLVGDDLFVTDPERLGRGL
                     REGVANAILIKLNQIGTLTETMDVMRMAADARYGRVVSHRSGETEDTTIADLAVGLGA
                     GQLKTGAPARSERVAKYNRMLRIEEELGDVARYGGGSLIRRSGP"
BASE COUNT         2817 a         7209 c         6640 g         2910 t
ORIGIN      
        1 ccggcctggg ctgccagctc ggcgagaacg cgccgccggg gcccgaggtc agcgccgcct
       61 gccccgagcc atcggcgttc atgatgtgga tctgcggttc ccccccaccc accggaaccg
      121 ccgcatacgc gatcgcggcc ccgtcgggcg accaggcggg atcggactcc tgcaggtccg
      181 gggtgttcgt caggtcgatc tgtccggtcc cgtcggcgtt catcaagtag atctccgacg
      241 tgccgtcctc ggagcaccgg acgaacgcca gcctggtgcc gtccggcgac cagctgggat
      301 cggcgtccag gaggtgattg ctggtcagac ggagcgggtg ggagccgtcg gggtccatca
      361 ggtacagctc ggggaaccct ccgtcccggt tcgtgctgaa ggcgatccgg ctcccgtccg
      421 gggaccaggc cgggtcgtcg tcgtagctcc ccgacgactc gaacgtcagc cggacccgac
      481 ccctcccgtc ggcccggatc cggaatagcc gggtgggtcc gcgcgcgctc gtacgcgccg
      541 catagacgag cgtcgtcccg tccggcgacc acgccgggtg gcgctcggcc accggcgtgg
      601 cggtcagcct gacccgtcct cggccgttcg cgttcatcac gaacaggtcg ggccgagcct
      661 ggagcttggg gcgccgggtg aaggcgatcc gggtgccctc gggcgaccag gcggggtcca
      721 tctcctgcat gtccgcggtg ttcgtgaggg caacctcctg ccggctcgcc gggtccagcg
      781 tgaacaggtc gccctgcccg ttcgcccggt ccccgcggaa ggccagcagg ccgttcgtgg
      841 cggggtacgc cgcctcggcg accggctcgg gcagcgcggt cgtggcgagg gcggcgagga
      901 cgaagatgat cgagaggatc gctcggcgca tggcgggcct gtaggatgag ttgcccgtga
      961 acgctaccac ctcccgccgc ctggttctcg gcctcgccgt gacgatcgcg gtggccggcc
     1021 tgtctccggc ggccggagcg ggcccgagcc gggatcggga ccgcagcgag gcgcccctac
     1081 gcaaggtgat ccgcaggcac atcgccccgg gtttgatctt cacgaagatc gtccagaaga
     1141 agatcccgct gcgtacctac atcctgcgag ccgacgtccg gaccacgccc ctcacgatgg
     1201 acgtggcgct gggcggctcc gcgatgcccc agcgggatcg gaccagccgg atcgccaacc
     1261 gccacggcgc gatcgcggcc gtcaacggcg acttcggcac gttctccggt ggccccacgc
     1321 accccttcga ggaggacggc acgctggtgc gcagctcgat ctcgcccggg ttcatgttcg
     1381 ccgtctcgaa ggacgagcaa cagatgttcg tgcagcgccc cgtgcagtgg atgagcgcga
     1441 cgctgccgag cggcctggtc tggcccatcg cccgatggaa cgacgggccg ccggggaccg
     1501 gagagatcac cgcgaacacg cccgtgggcg gctcgctgga gccgccgccc cccggcgcat
     1561 gctcggcccg gttgctaccc tccgggtcgc ccgagccctc gagcaccggc gacggcatgc
     1621 agcagtcgtt cacggtcgac gtgacgggat gcttcgagga tgggctggcc cggaacggcg
     1681 gcgtggtgct ctcggccatc ccggcgtccg acgaggccct ccagctgctg tcgctcgcgc
     1741 ccgggacgcc ggtgatgctg cgatggacgt tcggctggac caacgtgtac gacgcgatgg
     1801 gcggcgtgcc acagctggtg gcggacggca ggaacgtggc cggctcgtgc agcggctcga
     1861 tctgcaaccg caacccacgg accggcgtgg ggatcacggc cgacggccgg gtgatcatgg
     1921 tggtggtgga cggccgtcag tcgaagtggt cggtgggcct gacgatgaac cagttcggcc
     1981 gcctcatggt gcgcctcggc gccgtctcgg cgctgaacct ggacggcggc ggatccagca
     2041 cgatggtcat ccgaggcaag gtcgtgaacc ggccctcggc cgggttcgag cggcgggtga
     2101 cgaacgcgct cctggtgctg ccgggcccgg acttcgacga accgtagtgc gtcgatcagc
     2161 tcgcgcccct gcggacgtgc tcgagggatc cggccatctt ctacctcgtg ctggcgacgt
     2221 tcgggttcgc ccggctcggg tacctggcga tcgccgtctg acgccggctc cgcggaccgg
     2281 cccacatagg acagggagcc actctctcct gcaaacgaag gccatggtgg cgaacaagaa
     2341 ggaaccggcc cctggaaggg ggccggttcc cagcagctgt ccgggtactc ggactagccg
     2401 agacgcgcca gcagcttggc cgcagcggtg cccagggccc gcttggggtg cttggcggtg
     2461 ccgcagctgc gcttgatctc gatgctcttg cagatgtcct tgcccctgcc tccggcaccg
     2521 aggtcgtctc ccgacccgcc gaagagacgg tcgttcccgt tgccgccgaa caggttgtca
     2581 tcaccagagc cgccacgggc cgagtcgttg cccgggccca agcggactgt gtcgggtcca
     2641 cccccggcgc gcaccgaatc acggcccctc agcgccttga taaggttgct gtcctcgctg
     2701 ccagtgatgt tgtcgttgaa cggcgagccg atgacgctct cgatgttgga cagcgcgtcg
     2761 tccccgctgg gggcgctgcc ggtgccgagg gccaggttga ccgtcacacc acccgtctcg
     2821 ctacggtagg agacgaggtc ggccccttcc tcaccatcta ggacatccgg accgggacca
     2881 ccgttcaggg tgtcgttgga gagaccaccg tcgatcatgt cgtggcctgg gccaccccac
     2941 aggctgttgc ggaagtcgtt gcccgtgatg gtgtcgtcgc ccgagccgcc gatcgcgttc
     3001 tcgacgtcct ggccggggaa cagcgctccg ccggacagat cgatcgtgac gccggtggtc
     3061 atcgcgctgg catcaaccgt gtctcggcca tcgccgccgt agaacacgga gctgccgtcc
     3121 cagatcatgg tgtcgttgaa gtcggatccg acgaactcct cgatgtcggc gaactcgtca
     3181 tcgccctggc ccgtggccgt gccggccgcc gggtcgatgg tcacgccagc aagggagctg
     3241 gagtagtcca gcgtgtccgt gccgcccttg ccgtcgacgc tgtcgtcgcc ttcaccggga
     3301 acgaacgtct cgtcagcgac ggtgccgacg atgcagtcgt ctccggatcc ggtgatgtac
     3361 gtgtcgaagc catcgacgtc gtccatctcc tcgtcggtct cacctgcctc gcagtcggtt
     3421 gcggcgtcca ggttctcacc cgaaactgcg ccgccgccgg ccacgagcgc cacgtccgtg
     3481 acccgatcgc cgtagtcgat ggtgtcgccg gcgtttgacc caccgctcag cacgtctgct
     3541 ccattgacgg ccgctccctc gtagacggtg tcggcgccgg cattgccgtg cacctcatcg
     3601 tcgccggcgc caccggccag ccaatcatcg ccggcgtctc cgtacaggga gtcgtcgccg
     3661 gcgccaccgg tgatggtgtc gtcgccgtcg ccacccccgg cagagaagga cggaccagtg
     3721 gcggtcagcg ccgagccgtc gatggtgtca tcgccgtcgc cgccgtacac gtttacgtcc
     3781 tcgacgccga acatctggcc cggggccccg ttcatcgtga acgcggaatc accaacggtg
     3841 acgctgccgt ccacgccatc ctcaaggtgg atctcgaggg cgtcgccggt gccggttccc
     3901 aggtcgatgg cccaggcgat cgacgccggg aagctgacgt catggttgtt gtcgatcacc
     3961 agcgtctcgt cggtgctggg cgtcccgagc acgacgatcg aggtggtgtt gctgatcgtc
     4021 gcgcttccgc acgccgagcc gcctagctcg atcgccccgg tcgtcccgtc cacggtcatc
     4081 gtgaccgtgt cgccggagtc catcgtcacc gccaccgtat gggtgagcgg gttgaacgcg
     4141 cacgtgctca ccgccgacgc cgccggcgct ccgatcaggg caagtgcttg gaacgctcca
     4201 agcgatcctg ccgttaccac cgcggccgcc acccggcgac cgcgcttacc gaacaggttc
     4261 atgcgacccc ccttcctccc tctgcgctcc cgccctcgtc gcacaccgca acgagaggcc
     4321 ggtgcaggaa caacggtccg gacctatccc tgacacccga ccgagccggt catttcaccg
     4381 cccagcggtc cgggcgtcct tgcccgtagc cttgtgcctc acccccggcg gtgggtgcct
     4441 gacagaggtt ccagctccac ggaaccccac caatgcaaac gaacaacccg accaaaggtt
     4501 gcgggttaga gagacatccg gcgtaggtcg ataggcccgc cgctgtagat cgatcgagca
     4561 tcccgggtgg tgaggcggcg ggctaggcct cgaacaggga aacgtcggtc gcatacaggc
     4621 tggtgccatc ggcccagaac cgcaccttga actggacgct gccctgcacg ggaaccgctt
     4681 cctcaccggg ggcatcgtcc aggtggtacg ggcccacgaa cgtgtagtcg tagccgccat
     4741 cggaagtcgc cccgcccgag ctcaggaccg cgatcccgga gtacgagtac cagcccagat
     4801 cggtggcgag cttgaactgg aaatcgagcc ggccggtggt gccctcggcc gagccggcgt
     4861 actggacata ggccttccag gagggctgac cctgggagtc gagcgcggat cccacggcca
     4921 cctgagcgat gctcacggtg tcgcccgccg tgcccttcac cttcgaggag gtgagccagg
     4981 ttgcggccgg gcagtctgcg caggatccgg cccaggggat cccggcctgg aaggtcatgt
     5041 cccagtcggg agcgggaccg atcggcgctg tggtgggctc ggccgagggg gactcggtgg
     5101 gctcgggctc gggcgctccg gacggctcgt cggcaggatc gacgtccatc gagggcgact
     5161 ccaccgcggc cggctcggag gccttctgct ggtgatgctt cttcttgtgg tggtgctgct
     5221 gcttcgtctc cacgagcacc acctgattgg gtctgatcgc gggggccgac acgggggtca
     5281 cgacctggac ggccgcgggc gatgtccagg gcttcgcctc ctcggctttg ggcgtgcgcg
     5341 ccacgaccac ccccgcggtg accgagcccg ctaccaggaa ggccatcgct gcagcggtga
     5401 ggcgctcggc gcccgaggct gccgccgccg tgacggccgg tgccgccgtg gccgcagtcg
     5461 tcgcctgcgt ggcggcctgg ctcgccacct ggccgccccc gctctcgccg agggttccga
     5521 tgagccgccg caacaggtgc ggtagccgcg gcgggaacag cacccatggc accagagcag
     5581 agaccccccg gcggccgttg ccgttcccgt tgccctgcca cgcccggcgg aacgagccct
     5641 tggcccggtg gatcagggcc ttggcctgtg cggccgtgat ctcgagcgcc tcgccgatct
     5701 cctggtggga gcggccctcg acctcgcgga gcaccagggc tcggcggtgg tggtcgggga
     5761 gctcttcgag tacggccaag accctctcgc gctccagcac ccgctcgacc agctcgaacg
     5821 ggtcctgccc gccgtcgacc tggagatcct cgatacggtc ccgggcggcc acgccctcct
     5881 gcggccggcg ggccctggct cggagcttgt cgagcgagac gttcgtcgcg atgcgggcca
     5941 cccacgcctg gagctggtat cggccattga acttggggag gccttggtag acgcggagca
     6001 tggtctcctg cgtcgcctcc tcggcgtcgt cgacgctggc gagcagccgg aaggcgatgc
     6061 tccaggcgag ggcccggtac cggcggaaga tctcctggta cgcctccgcc tcgcctgcct
     6121 ggaagtccag gaccaggccg cgatcgtcca ggtcccgata ggacccgttc cccagctcag
     6181 aaaccccgat ggccatgctt ctcctgctaa ctcaaacgtc cgatgtgttg tcgaggttgc
     6241 cccagcaggc caaacgaggg ctcggcgccc agcacgcgcc gacgatatca cggccgctcc
     6301 ttgcggcgtg tatcctttcg cccctcgccg aaggtgccgt tcgacccacg gcgcgtgtcg
     6361 cgcccccact gcgggagacc catcccgccc tcgtggcccc acgacacgtg tgatgtatcg
     6421 acggccaacc cctggtgcct tcagcggaag gggcccggga tccgaagcgg ccgagggggg
     6481 gtgctacggt ctcggacatg gcaaggatgg gcatcccgac gatccgccgg cgagcgggcc
     6541 gcccgccggc ccccccaaca cggtgggccc accggtgatg gagcctccga tcgccggggt
     6601 gcggatcgtg ccgctgaccc tgcacgcgga cggtcgtggc tcgctgaccg agatcttccg
     6661 ccgttcgtgg gtgcccgggg cccgagaggt ggtgcaggtg aacctctcga tctccgactc
     6721 cggggtgctt cggggactgc actttcaccg ccggcaggcc gactactggt gcgtcctgtc
     6781 gggtcgagcg ctggtggggc tgttcgacct ccgcgagggc tcccccacgc ggatgcacaa
     6841 ggccgaggtc cccctcgacg ccgagctcga gcgggtcggc ctgtatctgc ccccgggcat
     6901 cgcccacggc ttctacgccc agaccgagct ggtcctggcc tacctggtgg acgggtactt
     6961 cacgggcgag gacgagctcg ggttggcctg ggacgatccc gaggtggcga tcgcctggcc
     7021 caccgatgcc ccgatcctgt ccgagcgcga ccgctcgaac ccttccctgg cccaggccct
     7081 ggcccacgcg cccacctttg tgggctgaga ccagaggtct ggcgggtcgt tccagggatc
     7141 ggggcacagg gccttggcgc atcgtcatgt caacccggct cggctacggg atcgggatcc
     7201 ggtcacccct tcgaagcatg ctcgccccag cccctacgtg atgagcagtc ggaccaggcg
     7261 gggcaggtag tgctccgtga gcgactggct gagcgatatc caggtgtcca gtgcgacggc
     7321 caggtcctgg atcgccttca acatctcctg ctgtcgctcg aacagcgcct ccatcgatcg
     7381 tcccgcgggc agctgcgcga tgccctgctc gttccaggcc caatgttgaa gctcggcgag
     7441 ccgctcgtgc agccgggcgg aggcctcact gatgtcctgc agccggtgac cgacttcccg
     7501 tggcacgttc acctcccgtg gacccacccc gacccccctt gtcgcctgcg ggggaggaac
     7561 cggaacccgt tctcgagctc gatgtggggg ctcgatcctg atgcgccgag cggcccgggg
     7621 tgaccgcgga cgccgaggac ccactcgagc cgtgccgtgc gtccggccga tcgcaccgtg
     7681 gaaccctggg gaccccttcg cgccggagtc cccgcgcggc ctgcgacgcc gccgcagaga
     7741 cctccctgcc ggtccggcaa cccctccgcg aggagcggtg agccgtcgcc gagccggccc
     7801 caccgtgcgg gaccgtgtcc ggccaactcc tgctctcggg cggagccgtc ggtgccgcgg
     7861 gcaggtccac gccgaggtga ctcgccgcgt gagccagcgc atcctggacc ggggccggga
     7921 ggacccccgc cgacgccagc ccaccggtca gcaccagggc ggcagccagc gccagcgcgc
     7981 cgatcctcct ccgacctgcc ctccggcccc gaggcctggt ccaccgttcg ccgatcgggt
     8041 ccggcgctcg atcgggcagc cgacgcacca cgtgctgcca gccgatccgg ggatcggggg
     8101 tcgcctccga ggtcagccgc tccagccgcc gatgcagcct caggagatcc tcgacctcat
     8161 cgtccgggtg acgggccttg atctcctcct cggagatcgc gtgcatctcc catcctcgaa
     8221 gatccgcctc caggccctga ctcatcgctc ctccaacatg gctcgcaggg accgcagtgc
     8281 ccgccactgc accgagtcga ccgcgtcggc ggtccggccg atcgccgtgg ccgcctcggc
     8341 gctggacagc ccgatcagga acttcagttc gagcacctgc cgctcctccg aagacaggtg
     8401 gcccatggcc tcggccaggc tcacccgctc gtccctctcc gatccctccg gctcctggac
     8461 cggttccccg tccggctggc tccgccgact ccctgccagc tcgtccagca catggagcgc
     8521 gatgccgtag agccacgccg cgaacggcgt tcccgcatcg cgataacgag gcaggtcccg
     8581 ccaggcccgc acgaaggtct cggccacgac gtcctccgga ccgtccggca catggaaacg
     8641 ggccacgcgg tagagggctg ggtgataccg ggcgtagagc agagcgaagg caacccgatc
     8701 cccctgcctg gcccgcgcga ccagggggct cgcctgcccc gacgccgccc gatcgtcgac
     8761 ctggcccgtg tggaccacgt ccctggaagc cgagatgcct gggatcgatc gggtcgccac
     8821 cccatacccc tcccactgat ccgggatgac taggtatcgg caaccggact ccatcccctg
     8881 agtccctcgg atgggggaga acgaccgatc cgccatccga ggccggtcgc cgacgcgggg
     8941 accagtcggg cgatgaggcg tagccgccca tccccaccgc ggcccggatc ctcgcgcccc
     9001 cgcttcagga tcgccacggg catgccgaca cctgctccat ggaggggacc cggctgtccc
     9061 gtcgcgtgct cgccgccctg atcgtcggca cgctgatgct cgccctcgta ccaggcctgt
     9121 cggacgtcgc ggcgagggcc cagaccgccc cctcccatcc ccagccccag ctcgatcgac
     9181 agcttcagca ggcccgcaag gacctgcgga agacgcgcca ccggatccgc gacgagctgc
     9241 ggcgcctgcg gaagatgacc aagcagatgg accacctggc cacccggatc tcgaagaacc
     9301 gctcggcgct ggatcggacc aaggcgcaca tggagaagct gaagaaggag atcgccaggg
     9361 tgaaggcgcg cctggcgtac ctggaggccc tgctggccga gcgctcacgg caggcataca
     9421 tcctgggccc cggcacgccg ctcatgtacc tgctcacggc ctcctccgcc gccgacgccg
     9481 cctcgcgcat cgtgatgctc gacgagatga accgtcgcga cgccgtcctg gccgaccagg
     9541 tggaccaggc cagggccgat ctgggcttcc gtcgagagtc cctcgccagg gtgaagttcg
     9601 ctcgtcggat cctggcgtac gccctcaagg acgcccagaa gcagctcgcc gccaagatgg
     9661 ccaagaccca gcgactctgg aagcagctca aggcccacaa ggtgctggtc ctcgacagga
     9721 tctcccggct gcaccccttc gccatgtgcc cggtccaggg gcccgtcgcg atcgccgacg
     9781 acttcgggat ctgggtacac cggccgaaga attggggcgg cgatcacatc caccagggca
     9841 acgacatgat ggcggcgatg gggacgccga tcgtggcccc cttcgacggg gtggcggtgg
     9901 acgcaacgaa ccatatcggc gggacggccg tgaaggtcat cggcaagtac ggctacgtgt
     9961 acaacgcgca cctgtctcgg ttcgggcagc tcggcccggt gacgaaggga acggtggtcg
    10021 gctacgtggg cgcgaccggg aacgccggcg gcccccacga ccacttcgag tggcacccgg
    10081 gcgggggcga cgcggtggac ccgcatccct tcctcatgca ggtctgctag cgccgagcgc
    10141 acggcccgca ccggctcgca tgcgatccgg ccccgcctcg acttcacacg gccggttccc
    10201 ttccgcacga ggacgccacc cgcgtaggcc tccggcactg ctagcttcga aggaggacgc
    10261 atccgagagg aggccacatg ccggtacgcg aagcggaagc ggagtggacg ggaaccttgc
    10321 aggaaggcga cgggacgatg cgcttcggcg gtggggcgtt cgagggccga tactccgccg
    10381 cctcacggtt cgaagagggt cccggcacca accccgagga gctcatcggg gccgcccacg
    10441 cggggtgctt ctccatggcc ctcgcggggg cgctcgtccg cgccgggttc ggccccgagg
    10501 ccatccgcac gggggccagg gtccacatcg agaagggcga ccccggatgg gcgatcacca
    10561 agatcgacct gcggaccgag gccacggtgc ccgacatcga agatgccagg ttccacgaga
    10621 tcgccgagga ggccaagcgg gactgccccg tgtccaaggc cctggcggcg gtccccatcg
    10681 agctcgtcgc gaccctggcc tcctgaccgg gtcaccggac accgtccgtc tcagccgggc
    10741 gcttggagct ctccaggagg cgctcggtcc gtgccgccga agacaggttc cccttgcggc
    10801 ggtacagacc cagcgcctcc tcccgcgctg cggccgcgtc cggatcgttg cccctcgtcc
    10861 ggagcacctc cgccaggtcc aggagcacgt cggcccgcat gttgagatcg tccgtcccct
    10921 ccgccaggga cacggcctct cgggccgatc gctctgcggc cgccggctcg ccccgtgccg
    10981 cgagcacccg tccccgcgcg ctgcggagca gcacctgggt gctcaggtcg tcctcggccg
    11041 cctcctctgc gatctggagc cagcgctccg cctcggggac gcgctcgggg ccctgggcca
    11101 ggatggcgtg ggccagcagg gcggcactgg tcgtccggta tccccgctcc ccgagctcgg
    11161 tcatggcgtc gaagcctgcc cgcagctcgc gctccgccgc ggcgtgctcg cccgccagtc
    11221 gttccacgaa cgcgagcgtc tcggacacga acgccgctcg cagggcgagc ccgagatcct
    11281 ccagcaggtc ggcagcctgg cgagccagat cccggccctc ttcgtaccgt ccctgcatcg
    11341 ctcgaaggga ggccaaggcc cggagggcgc gagcctgcac cgaccggttg cgtccacccg
    11401 aagccaggac ctggtcgcat cgctcgatca cctcggccac gggggccggt ccgtagacgc
    11461 ccgaccccgt gtactgaccg agcgcctccg cctcctccca ggtggcgccg gcccgccgag
    11521 cgtgctccag ggcccgtccc agggcaacgt ccacggcccc gtaccgggag cgggtccact
    11581 ccacgtcccc cagcagccgc caggccctgg ccaaccccag atcgtcgccc agctcctcga
    11641 agaccgggat caccgcttcc agctgcctga gggcgtggct cgaccgctcc tcgggctcgg
    11701 tggactcctc gagcaggagc ctcaccaccg ctgcatgggc ggccacgccc cggtcgccgg
    11761 ccgcctcggc ctcctcgatc gcggccgcca ggaggccgtc cgcccgtgtg aggtcgccgg
    11821 tctccatgag cgcctccccc agatcgggca ggagggcccg acgaccggcg tcccccgccg
    11881 ggagcagcga caccgctcga tcgagcagat ccgcggcagc cggcaggtcg ccccgggcct
    11941 gcgctcggcg tccggccgcc gccaggtgcc cggccgctcg accggcgagc tggtgctcgg
    12001 cctcccccac cgcgcccagc tcggcccggt acaggtaggc gcgttccagg tggtagccca
    12061 gcacctcctc gaactcgacc cggcgatcac cggccgtggc ggtgagccac cccgcgaacc
    12121 gctcgtggag ctcggcccgg acgcccttgg gggtgctccg gtaggcggcc tcctggacca
    12181 ggaggtgatg gaagcggaag gcgtcctggc cggcgaacgt cgaccggccc gggcggatga
    12241 ggtccttgcg gaccagcgcc tggaggtgac cgcccacgtc gcggcggacc cgtgcctcca
    12301 gcatgtgcga cacggctccc caccagaaca ccttcccgat caccgaggca caccggatca
    12361 cggttcgctc atcgggcgtg agccggtcca gtcgggcgct gacgagggcc tggatggtgg
    12421 gcgggaccga gatgtgggac agatccacgg cggtccacgc ctcgtcctct cgccgaacca
    12481 tcccgtcgtc ctccagcatg cggagcagct cttccaggaa cagggggttc ccgccggccg
    12541 cctcggtgat ccgtcgggtc gcccggggat cgcccgctcc gcctccgagg atgccccgga
    12601 gcagctgctc gctctccgcc tccgtgagcc gggtggggga cagacgatcg ccccgcaccc
    12661 ccgtgcccca ggccggtcgg aggtcctcca ggtcgggccg ggccgcgcag agcaggagga
    12721 ccggggcatc gcggatccac cccgccaggt actcgaccag gtccaggaac gtgggctcgg
    12781 cccattggat gtcgtcgaag aggacgaccg cggggccctc gcggccgatc cattccagca
    12841 ggcgacggat cgcccagaag gtctcctgga tgtccacggc ggcgaccgcg agccccatga
    12901 tcgaacccac ccgctcggcc accagatcgg cctccggcgc gccggagagg accgtgccga
    12961 tcttgccggt ggctacggct cgcggatcgt cgtcgtggat gtcgcaggcc tgcttcacga
    13021 tctcggcgac cggccagaac gtgatgccct ccccgtaggg gaggcaccga ccctgaagga
    13081 tcgtagcccg tccctccagg gtcgcggcga actcccggac cagccgggac ttgccaacgc
    13141 ccgcggagcc aacgaccgtc accagacggc aggaccgctg ctcggccacg gcggcgaacg
    13201 cctgccggag cgcgtccagc tcggccgccc ggtcgaccag cggcggatcg ccgcgttcgg
    13261 cgagcagggg cccctcaggg aggtccgaga gcagccggta cgcgctcacg cgctcccgct
    13321 tgcccttgag gaccaggggc tcgaccggct cggccccgac caccccgcgc accagccgat
    13381 aggtgtcccg acccaggagc acctgcccgg gggaggccgc ctgttccagg cgcgccgcaa
    13441 cgttcaccgc atccccgacc accagcgcct gcccggccgc cacgtcaccc accaccacct
    13501 ccccggtgtt gatgccgatc cgggtatcga ggctcacgct ccagccggcc cgcagatcct
    13561 cgttcagcga gggcaacgcc cggtgcatct ccgaggccgc gcggacggcc cggagggcat
    13621 cgtcctcgtg gacggtgggc acgccgaaca cggccatcac ggcatcgccg atgtacttct
    13681 ccaccgtgcc gccgtggtgt tccaggatcg tccccatccg gccgaagaac cgcctcatga
    13741 cctcgcgcac cgactcggga tccagctgct cccccaggga cgtcgagccg accacgtcgc
    13801 agaacagcac ggtcacggac ttgcgcgacc cgggcggcgc cggctcctcc gcgggcaggg
    13861 gcgacgcgca ccacatgcag aacctcgccc ccacggggtt ctcgcgccca caggccggac
    13921 agggcaccgg catggcgaga gtctaggcac tccctccgag cccttgctcc ccggtgcatc
    13981 ggcggcctag gctcggtctt ccgcgaccgg agggagcgtc ccatggagat gtcgatcgag
    14041 atcgaccagg agttctcggt gatcggcacc gatgaccgcc tcagcacccg gttcgcggag
    14101 gcctcgtcca cctccccggt ggtcgtggag cagcagctgt acgaggacct ggggagcgac
    14161 gaggagtggg gggaacgggt cggcgagctg cgcggggtgt gcttcccggt agggcagggt
    14221 caggccctct gcaacgtggt cctccggttc gacggcaagc tcgggaaggg ctcggtgagc
    14281 gcggccggga tcttggacgt ggccggggag gccgacctgt cgggcgcgat ggcgatctcc
    14341 ggaggcaccg ggcccttccg gcagcggcgg ggcgagctcc aggtcacgag ctggaacccg
    14401 aagcgctggc gcctggcggt ctgatccgcc acgggcacga acggatccgt gcccggggtg
    14461 ggagtcgaac ccacatgccc tttcgggccg gcgtgtttaa gacgcctccg tattccgttc
    14521 cggcacccgg gccccaacaa ggataggcgg tggggccggc cacctatccc gaaacgtcga
    14581 tcgtgacctt gtccaggtac acggcctgct tcggctgctg gctcgatccc tggaggggaa
    14641 ccccggcgat ccgccgcacg acgtcgagcc ccttcacgac ccgtccgaag atcgtgtagt
    14701 tcggggtcgg gtccagggtg tgccccttgt ccccgtagac cacgaagaac tggctcccgc
    14761 cggtgttggg cccggcgttc gccatggcca cgaccccggg gccgtagctc tccttgcccg
    14821 agagctcgtc cttgatgctg tatccgggtc cgcccgtgcc ggtccccttg ggatccccgc
    14881 cctggatgat gtccaggctc gtgtcgatcc gatggatcct ggtggcgtcg tagaagccct
    14941 cgcgggacaa gaagacgaag ctgttcacgg tctggggggc ctgatcggcc aggagccgca
    15001 cccggaacgt cccgcacgag gtctcgatcg tggccgtgta ggtcttggag gcatccaggc
    15061 gcatcggcgg cggccccgcg aactggggct tgggcttcgt cgcgcccttc ggggcctgcg
    15121 ctccgcaggc caccaccttg ggcccgggcg acggctccac ggttccggtc ttcgtccccg
    15181 gagccgcagc gctcggggtc ggggtgccgg acgcggcgct gttgggcccg ttcccggtga
    15241 ggaactcgaa gccgaggacg gccagcccga tcagcgccac cccgaacgcc acgccgatga
    15301 tcgtggcccg tttgcgctgc tgccggcgcc gttcctcgta tcggcgctgg gccagcttgg
    15361 cgagctgccg gttcctgctc ttcctcgaca cggcgtggaa gactaccgcg cggtgagcag
    15421 ctcggacgcc aggccctcga ggatgtccgt ctccgagacc agcgctcgtg cgaacccgaa
    15481 ccggtgcatc acctcggcca gcaccacggc gccggccacg atgacgtcgg cccggccggg
    15541 ggccatgacc ggcagcgccg cccgctccgc cgtgctcatc cgccggagcc ggtccaagac
    15601 cgcccgaacc tccaccaggg acagccacgt gcggtggatc cgctcgggat cgtagaaggt
    15661 caggtcggcc gccaccgccc gcagcgtggt ggccgtgccg gccaccgcca ccatcgtccg
    15721 agcctcctgc acgggcacgt cctgctccac ctcatcgagc acccggccca cgagctgtcg
    15781 catgctcgtc agctcggcca gggacggtgg atcgtctcgt accagccgct cggtgagccg
    15841 aacgctgccg atccgcttcg acacggcggc gtggggacgg tcggagccca ccacgaactc
    15901 ggtggacccg cccccgatgt ccaggacgaa gtacggggcg ggctgctcca gtcccccggt
    15961 ggcgcccagg aacgacagag cggcctcctc ccggccgctc aggatccgga gctcggcgcc
    16021 ggtgagctcg cggacggcac gctcgagctc ttcgcggttg ccggcctctc ggaccgcctg
    16081 cgtggcggcg acccggatcc gctcggcgtg cagggctcgc gctcgtcggg agtaccgggc
    16141 cagcacgtcc acggtgcgcc ggagcgcctc cggatcgatc cggcccgtgc gatcgacgtc
    16201 ctgaccgatc cgggtgacga gcatgtcgcg ggcgaacgcc tccaaccctc cgcccgaggc
    16261 ctggctcgcc accaggagcc ggatcgagtt cgtccccagg tccaccgccg ccacccgacc
    16321 ggggcgctcc tccggatgca ccggctccac acgcgccgcg gcccaggccc ccaccggatc
    16381 ggcacctccg gccaggtggt tggcgtagtg ggcgtgcagg catttgatcc cggctcgagc
    16441 gccacccacc ccgccggacg atctcccctg cgggtcgttg cgtgtccgct cgagcgcgta
    16501 ctcgcggtgc gcacgctcca gctcccgtgc gaagggctgg tcctctcgcg cccggtcgtt
    16561 cagccggccg atccagccct ccgactccag gcgggacagg gccctcacgg cctcggggca
    16621 cgtgagccag aacaaggtgg ggaagggatg cccccggggg tcgaaagggc ggttgcggat
    16681 caccagcgga tgcccgcccg ggcagcgggc aaccaccgtg aacggggtga cgggctctcg
    16741 acccagctgg tcacgaacgg cgggcagatc gctgcccagg agctcactca aacgatgccg
    16801 ttacttgcgc ttgtgctcaa cggagcgctt gtagtcgacc tggcgctcct cgctctggcg
    16861 catgaacttc ttgagcatcg actcgaaacc cgggtcggcg ccgcgccgac tccgttgcgg
    16921 agcagggtcc tggagggcct tgatggacag gtcgatcttg ccgtcttcct tgatggcgat
    16981 gaccttggcc tggaccacgt cactctcgcg gaggtgatcg tggacgtccc tgacgtagtt
    17041 gcggtcgatc tcggagatgt gcacgagtcc gaccttgcct ccatcgagcg tgaggaacgc
    17101 tccgtacggg gtgatccgcg acacggtccc ctcgatgacc gcaccgacct cgaggtctgc
    17161 gatgaccgct cctttccgct ggtgagcagg cccgttgccc gctcacgcta ccgagagcgc
    17221 cactatccca aggcccgggt tcccccgtca ataggcgccc ggccgatacg ccgcttccgc
    17281 gccctccaag ggggcggagg tgccctcgct cttggggacg gtaccctgat gcccggcatg
    17341 cgcaggagat ggctcgcagc ggtgctgctc gccgggatcg tcgccgccgg atgcgcgagt
    17401 gggacccccg gccgtccggg ggggtcgatc ccggcgccga cctcggcgtc cgctgcctcc
    17461 ggctcggatc ccgcgacgat cgagcacgtc gtggtgatcg tgatggagaa ccacgagtac
    17521 gggcggatca tcggctcacc gcaggcgccc tacctgaacc ggttggccag gaggaacgtc
    17581 ctgctgaccc acgagtacgc gatcaggcac ccctcgctgc cgaactacct cgcgctgacg
    17641 gcaggctcca cgttcaagat ctcgtcggac tgcacgagct gccgggtgaa ggcgcgcaac
    17701 ctggtggatc agctggaggc tcatggcatc tcctggaggg cctacatgga gtcgatgccc
    17761 tcgccctgct tcacgggggc cacggcgggc acggcaccca acgactacgc caagaaacac
    17821 gacccgttca tgtactacga cgacatccgc aaacggcccc gacggtgccg ccaggtcgtg
    17881 cccttgcgca ggctggcccg gaacctcgag gcgggcaccc tgccccggtt cgcctggatc
    17941 accccgaacg agtgccacga catgcactcg tgcggggtgg ccaccgggga cacctggctg
    18001 aagcgatggg tgccccagct gctcccggcc ctgggatcca acgggatcct gttggtcgtc
    18061 ttcgacgagg gctcgtcgaa cgccggctgc tgctccatcc cgggaggcgg gcacgtggcc
    18121 gcgatcatcg ccggaccggg cgccggccac ggcatccgga tcaggaccgc cctggaccac
    18181 tactccctgc tccggctcat cgaggacgcc tgggggctgc gtcacctccg ccgagccggc
    18241 gacgccgtca ccccgacggt cacgggatgg tcctcgggct gatccgatca gcagtcggcg
    18301 ggtcccccgt ccgccgtggg cgggccgatc acgaaggcgg tctcgcccgg ccgcaccatg
    18361 ccgaggcact gacgggccaa ccgctctagc tccacgggat cgtgcaggcg ggtgacctgc
    18421 cgttgcaggg tggcgttcga gccctccagc acctgcacct gacgttggag gtccctgatc
    18481 tggccccgct gggacaggta cgcccgggcc ggtcccaccg cgaggagcgc cagcagcgtc
    18541 acggcggcga tgaggaacgc ggcccgaccg gtcaggcgga cacgagtgcc ggtcgccgcc
    18601 tcggcccgga ccgtcacggc ccgctccgcc ggatcagcga gccgcccccg tagcgggcga
    18661 cgtcccccag ctcttcctcg atcctcagca tccggttgta cttggccacg cgctcgctgc
    18721 gggccggggc gccggttttg agctgccccg cccccaggcc cacggccaga tcggcgatgg
    18781 tcgtgtcctc ggtctcaccc gagcggtgcg agaccacccg tccgtaccgc gcatcggcgg
    18841 ccatgcgcat cacgtccatg gtctcggtga gggtgccgat ctggttcagc ttgatgagga
    18901 tggcgttggc caccccctcc cggagccccc ggcccagccg ttctggatcc gtgacgaaca
    18961 ggtcatcgcc gaccagttgg cagcggccgc ccagctcccg ggtgaggtgc ccccacccct
    19021 cccagtcgtc ctcggccagg ccgtcctcga tcgacacgag ggggaacccg tccagcagtg
    19081 ctcggtagaa cccgaccatg tcggccgagc tccgatcgct cccctcgaga cggtaggtgt
    19141 cgtcgcggta gatctccgac gcggccgggt ccatggccag ggccatctcg tcccccgcct
    19201 cgagccccgc gctcccgatc gcctcgacca tgaccgacag ggcttcggcc gcggtcccga
    19261 tctgcggggc gaacccgccc tcgtccccga cgccggtggc cagaccgcgc cccttcagca
    19321 gggacttcaa cgcatggtag gtctcggcgc accagcggag agcctcggag aaggaggcgg
    19381 cgcccaccgg gacgagcatg aactcctgca gctcgagctc gttgtccgcg tgcacgccgc
    19441 cgttgatcac gttcatcatg ggggtgggca ggaggtgggt cccggagcca cccaggtacc
    19501 gatacagggg gagcgccacg ctgttcgccc cggcgtgggc caccgccagg gacaccccca
    19561 ggatcgcgtt cgcccc
//
LOCUS       DASQQQ010000113         7272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_225017, whole genome shotgun sequence.
ACCESSION   DASQQQ010000113 DASQQQ010000000
VERSION     DASQQQ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225017"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VE646_04730"
     CDS             <1..227
                     /locus_tag="VE646_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HZD79329.1"
                     /translation="VEARLVSYNVSSVTGGSDALGDVVVQLDVAGRRVTGRGVATDVV
                     EASARAYLAAINRSLQAPVGSASTTDAWSA"
     gene            224..1282
                     /locus_tag="VE646_04735"
     CDS             224..1282
                     /locus_tag="VE646_04735"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HZD79330.1"
                     /translation="MSGSYRVGVLPGDGTGPEVVREGLKVLDAVAEQEGFRVERETFD
                     LGGERYLRTGEVLPDEELARLRACDALFLGAVGHPDVRPGILERELLLRIRFELDQYV
                     NLRPVIRYPGVETLVPSHTEHDVDLVVVRENSEGLYSGAGGFHRKGTAHEVAVQESVN
                     TRHGVERIVRYAFQLAMRPDRRRKLTLVHKTNVLTYAGDLYRRVVDEVGREYPEVVTD
                     YVHVDAACIYFLDQPSRFDVIVTDNMFGDIITDLGAMIQGGMGIAAGANINPGGVSMF
                     EPIGGTAPDHTDKGTINPLAAIGAVQMMLEVLGESAAAGRVGGGIRFACSKLHSMRAG
                     EMGYATSEVGDLVVEAAS"
     gene            1279..2862
                     /gene="cimA"
                     /locus_tag="VE646_04740"
     CDS             1279..2862
                     /gene="cimA"
                     /locus_tag="VE646_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HZD79331.1"
                     /translation="MSGTHRVELYDTTLRDGAQRAGLSYSIEDRLRILHKLDSLGFPY
                     VEGGWPGANPRDSEFFRLAAKERLEHATLTAFGMTRKAGERAQDSAVLRELLEAGTVA
                     ICLVGKSWDLHVTDALRTTLEEGVAMVRESIAFLRSEGRRVFFDAEHFFDGYRRNPDF
                     ATAVLAAAEEAGAERLVLCDTNGGCLPAEVARVVGEVKETTTVPLGVHVHNDAGCAVA
                     NSLVAVESGVVQVQGTVNGYGERTGNADLVPIVANLELKLGRAALPAGSLERLTELSH
                     YVAEVANLTPDSHQPYAGRYAFTHKAGLHTSGVSRFAEAYEHVPPASVGNHRGVVASD
                     LGGRSTIVMKAHELELELAEDAIPGLLDELKRREARGYTFEVADASLELLVRRHDGWR
                     QPFFRIDSYRVHVEQRGEDEALAEATVKVWTNGNRHIESAEGNGPVGALDNALRKALL
                     NEYPELEHITLEDYRVRVLDENVGTGSTVRVLIDSSNGQREWTTVGVSDNIIEASWEA
                     LTDGYLYGLLHPRQDGGAG"
     gene            2874..3392
                     /locus_tag="VE646_04745"
     CDS             2874..3392
                     /locus_tag="VE646_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZD79332.1"
                     /translation="MAVRIREAVPGDARAIAEIHVRSWQHAYRGQLTDEYLDGLKVED
                     RLERHRQSLEQPPADYRMWLAEQGGEVIGFACTGKSGDADADERTAELYAIYLTPDRI
                     GTGVGRELLDHAVADLTDRGFRTATLWVLRTNEVAHRFYEGTGWATDGTTSAERVDRE
                     MRPTVRYRRDLA"
     gene            complement(3371..4579)
                     /locus_tag="VE646_04750"
     CDS             complement(3371..4579)
                     /locus_tag="VE646_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79333.1"
                     /translation="MSDPARERIRVAIAGRIYRRPTLVRRFLEDDGFDVVDISSTLEG
                     TLAALAEQEPDAVVVGEGMSEDLANLRSAAPEARIVVMGGAGSPGPIADAHLPRGAAL
                     ARLTAALRAQFDRPEVAPSATRDGATSEPGPAPSEDRTAAARMGGLAAALTLVVAVAL
                     GVVGGPLSPTGIRALAPSIIRPIGRPAVASPLDLAQRELTGLADALGSGDDTAALTLA
                     RSLMDHLAEARSAGLNMTGIEGQVRAVLRPLLADVSPGVLSALRAILGDLLAPFPTPV
                     GGGVPAITRSSSGGSVAGPQPTAPTAGPVPGPSEGSSGVPVPSPSPEHTDRGRGVEPD
                     KPPKGGWHGGNPRRQGPKDRSHGHGSHGSDHARGQGRKRGGERGNDHGGDQGREHAGG
                     AEPRYARSLR"
     gene            4709..5515
                     /locus_tag="VE646_04755"
     CDS             4709..5515
                     /locus_tag="VE646_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018570424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZD79334.1"
                     /translation="MALTGIAGVGTYGSVRVLVGTSGWRYDHWRGRFYPQGLPTSRWL
                     SAYAERFATVEVNNTFYHLPKAETFARWAVQTPAGFVWSVKASRYVTHIRRLRDCGSA
                     VELLWSRAIELGPHLGPILFQLPPTLAADPARLAAFLRVLPTGMRAAFEFRHPSWLRP
                     EVLSLLDGAGAALVLADRPGARVRPLVTGGWSYVRLHQGRPDAPGYARRKLARWAERI
                     AGLPASEAFVYFNNDTGGAAVQDALTMRELLAARGIEWPRSPRSAPRDEG"
     gene            complement(5436..6029)
                     /gene="leuD"
                     /locus_tag="VE646_04760"
     CDS             complement(5436..6029)
                     /gene="leuD"
                     /locus_tag="VE646_04760"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HZD79335.1"
                     /translation="MEPFHIHTGTAVPLDRADVDTDQIIPAEHLKRIERSGFGPFLFG
                     AWREDPDFVLNRPEHRGASILLAGPNFGCGSSREHAVWALQDAGFRIVVAPSFGDIFR
                     TNCTKVGLLPVRLPEPVVRTLMEAVDRDPATPITVDLEALRVTAPGVDQAFQMDDFTR
                     RRLLEGLDDVGLTLRHEEQIADYEATRSPWLPTAPAS"
     gene            complement(6036..>7272)
                     /gene="leuC"
                     /locus_tag="VE646_04765"
     CDS             complement(6036..>7272)
                     /gene="leuC"
                     /locus_tag="VE646_04765"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HZD79336.1"
                     /translation="DLTVATMDHNVPTAPGPEDPVSVLQLEALRRNCAEFGITLFPTG
                     HIGQGIVHVIGPQMGLTQPGLVIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLR
                     QRRPAPMAVTVDGDLPPGVTAKDLILGIIARIGTGGGVGHVLEYRGSAIRSLSMEGRM
                     TVCNMSIEAGARAGLVAPDQTTFAYLEGRPGAPRGAEWERALDHWRTLATDADAAFDR
                     EVTIDAADLTPFVSWGTNPGQSVSIEAVVPEPTDETMERALAYMDLAPGTPMRQIRPD
                     TVFIGSCTNSRIEDLRIAAQVVRGRRVHEGIRALVVPGSMLVKAQAEGEGLDTVFTRA
                     GFEWRDAGCSMCLAMNADKLAPGERSASTSNRNFEGRQGAGGRTHLVSPAVAAATAVA
                     GHFAAPADLGIDLRTAKGA"
BASE COUNT         1039 a         2498 c         2698 g         1037 t
ORIGIN      
        1 gtgtcgaggc ccggctcgtc tcctacaacg tctcgagcgt gaccggcggc tcggacgcgc
       61 tcggggacgt ggtggtgcag ctcgacgtgg cggggcgccg ggtgaccggt cgcggcgtgg
      121 ccaccgacgt ggtcgaggcc agcgcccgag cctacctagc ggcgatcaac cgatccctgc
      181 aggccccggt gggctcggcc agcaccacgg atgcgtggtc cgcgtgagcg gttcgtatcg
      241 ggtcggggtc ctgcccggcg atgggaccgg tcccgaggtg gtccgcgagg gcctgaaggt
      301 gctggacgcg gtcgcggagc aggagggctt ccgggtcgag cgcgagacgt tcgacctggg
      361 gggcgagcgg tacctgcgga cgggcgaggt gctgccggac gaggagctcg ctcgactgcg
      421 ggcctgcgac gccctgttcc tcggcgccgt ggggcacccg gacgtcaggc cgggcatcct
      481 ggagcgcgag ctgttgctgc ggatccgttt cgagctggac cagtacgtca acctgcggcc
      541 ggtgatccgg tacccggggg tggagaccct ggtcccctcc cacaccgagc acgacgtgga
      601 tctggtggtg gtccgggaga acagcgaggg gctctacagc ggggccggtg gcttccaccg
      661 caaggggacg gcgcacgagg tcgcggtcca ggagtccgtc aacacccgac acggggtgga
      721 gcggatcgtc cgatacgcgt tccagctggc catgcggccg gaccgccgcc ggaagctcac
      781 gctggtgcac aagaccaacg tgctcacgta cgccggggat ctgtaccggc gcgtggtgga
      841 tgaggtgggc cgagagtacc cggaggtcgt caccgattac gtgcacgtcg acgccgcctg
      901 catctacttc ctggatcagc cctcgcggtt cgacgtgatc gtcaccgaca acatgttcgg
      961 cgacatcatc acggacctcg gggccatgat ccagggcggc atggggatcg cggctggggc
     1021 caacatcaac ccagggggcg tgtcgatgtt cgagccgatc ggggggacgg cccccgacca
     1081 taccgacaag ggaacgatca accccctggc ggcgatcggc gcggtccaga tgatgctcga
     1141 ggtgctcggc gagtccgccg cggctggtcg ggtgggaggc gggatccggt tcgcctgctc
     1201 caagctccac tcgatgcggg ccggcgagat ggggtacgcc acttcggagg tcggcgacct
     1261 ggtggtggag gcagcctcgt gagcgggacg caccgtgtcg agctctacga caccaccctc
     1321 cgggacggtg cgcagcgggc cggcctctcc tactccatcg aggaccgctt gcggatcctg
     1381 cacaagctgg actccctggg gttcccctac gtggaggggg gatggccggg agccaacccc
     1441 cgggacagcg agttcttcag gctcgccgcc aaggagcggc tggagcacgc cacgctgacc
     1501 gccttcggca tgacgcggaa ggcgggagag cgtgcgcagg acagcgcggt gctccgcgag
     1561 ctcctggagg ccgggaccgt ggcgatctgc ctggtgggca agtcctggga cctgcacgtg
     1621 accgatgccc tgcgtaccac cctggaggaa ggggtggcca tggtacggga gtcgatcgcc
     1681 ttcctgcgct cggagggccg acgggtcttc ttcgacgccg agcacttctt cgacgggtac
     1741 cggcggaacc cggacttcgc gacggcggtc ctggccgcgg ccgaggaggc cggcgccgag
     1801 cgcttggtcc tgtgcgacac gaacggcggc tgcctgcccg ccgaggtggc ccgggtggtg
     1861 ggcgaggtga aggagaccac caccgtgccg ttgggggtgc acgtccacaa cgatgccggc
     1921 tgcgccgtcg ccaactcgct cgtggccgtg gagtcgggcg tggtgcaggt ccagggcacc
     1981 gtgaacggct acggggagcg caccgggaac gcggacctgg tcccgatcgt cgcgaacctc
     2041 gagctgaagc tgggtcgcgc ggcgctgccg gccggctcgc tggagcggct gacggagttg
     2101 tcccactacg tcgccgaggt ggcgaacctg accccggatt cccaccagcc ctacgcggga
     2161 cggtacgcct tcacgcacaa ggccgggctc cacaccagcg gcgtgtcccg cttcgccgag
     2221 gcctacgagc acgtgccccc ggcctcggtc ggcaaccacc gcggcgtggt ggcctccgac
     2281 ctcggcggtc gttccaccat cgtgatgaag gcccacgagc tcgagctgga gctggccgag
     2341 gacgcgatcc ccggcctgct ggatgagctg aagcgccggg aggcgcgcgg ctacacgttc
     2401 gaggtggccg atgcctcctt ggagctgctg gtccggcgcc atgatgggtg gaggcagccc
     2461 ttcttccgca tcgatagcta ccgggtgcac gtggagcagc gaggtgaaga cgaggccctg
     2521 gccgaggcca cggtcaaggt gtggacgaac ggcaaccgcc acatcgaaag cgcggagggc
     2581 aacggcccgg tcggtgcgct ggacaacgcg ctgcgcaagg cgctgttgaa cgagtacccg
     2641 gagctggagc acatcacgct cgaggactac cgggtccggg tgctggacga gaacgtcggc
     2701 accgggtcga cggtgcgggt gttgatcgac tcctcgaacg gccagcgcga gtggaccacg
     2761 gtgggcgtct cggacaacat catcgaggcc tcctgggagg ccctcacgga cggctacctc
     2821 tacgggctgc tccacccgcg ccaggacggc ggggccgggt agcatctgcg tccgtggccg
     2881 tccgcatccg cgaggccgtt cccggcgacg ctcgagccat cgccgagatc cacgttcggt
     2941 cctggcagca cgcctaccgc ggacagctca ccgacgagta cctggacggc ttgaaggtgg
     3001 aggaccgcct ggagcgccat cgtcagagcc tggagcagcc cccggccgac taccgcatgt
     3061 ggctcgctga gcagggcggc gaggtgatcg gcttcgcgtg tacgggcaag agcggggacg
     3121 ccgatgcgga tgagcggacg gccgagctgt acgcgatcta cctgactccg gaccggatcg
     3181 gcaccggcgt gggacgggag ctgctcgacc atgccgtggc cgacctgacg gaccgagggt
     3241 tccggacggc cacgctctgg gtcctgagga cgaacgaggt ggcccaccgc ttctacgagg
     3301 ggaccggctg ggcgaccgat gggacgacgt ccgccgagcg cgtggaccgg gagatgcgcc
     3361 cgaccgttcg ctaccggagg gatctggcgt agcggggttc agcgccgccg gcgtgctccc
     3421 gaccctggtc gccgccgtgg tcgttcccgc gctcaccgcc gcgcttcctg ccctgccccc
     3481 gggcatggtc gctcccgtgg gagccatgcc catgtgatcg gtccttcggt ccttgccgcc
     3541 tggggttccc gccgtgccag ccgcccttgg gcggcttgtc cggctcgacg ccgcgaccac
     3601 ggtcggtgtg ctcgggggag ggcgagggca ccgggacccc gctggagccc tcgctggggc
     3661 cgggcaccgg ccctgcggtg ggagcggtgg gctggggacc ggccaccgag ccgccgctcg
     3721 aggaacgggt gatcgccggg acgcctccgc cgaccggggt ggggaacggc gccagcaggt
     3781 ctccgaggat ggcgcgcagc gcggacagca ccccagggga gacgtcggcc agcagcggac
     3841 gcaggaccgc tcggacctgg ccctcgatcc cggtcatgtt cagacccgcc gacctggcct
     3901 ccgccagatg gtccatgagc gatcgagcca aggtaagcgc tgcggtatcg tcgccgctcc
     3961 ccagggcgtc ggcgagtccg gtcagctctc gctgggcgag atcgagcggc gaggccaccg
     4021 ccggccggcc gatggggcgg atgatggagg gcgcgagggc tcggatcccg gtcggcgaca
     4081 gggggccgcc cacgaccccc agggccacgg ccacgacgag cgtcaacgcc gccgccaggc
     4141 cccccatccg ggcggcagcc gtccggtcct ccgatggcgc cggcccgggc tccgaggtgg
     4201 ctccatccct cgtggccgag ggcgcgacct cgggccggtc gaactgcgct cgcagcgccg
     4261 cggtgagccg agcgagcgcc gctccccgag gcaggtgcgc atcggcgatc ggccccggcg
     4321 acccggcgcc tcccatcacg acgatccggg cctcgggagc cgcggagcgg aggttcgcca
     4381 ggtcctcgct catcccctcg cccaccacca cggcgtccgg ctcctgctca gccaaggcag
     4441 ccagggtccc ctccaaggtg gaggagatgt cgaccacgtc gaaaccgtcg tcctccagga
     4501 accggcggac gagggtggga cgacggtaga tccggccggc gatcgcgacg cggatgcgct
     4561 cccgcgcggg gtcgctcatc gccccggttc cctccagggc atacaggcat cgtacccacg
     4621 ccatcgcgcc tggcccccgg gcccttcggg ggatttcagc gcccgacacc gtaggccggg
     4681 tgggggatgg aaccctggcg gacgggcgat ggcgttgacg ggcatcgccg gggtgggtac
     4741 gtacggatct gtgcgggtcc tggtgggcac cagcgggtgg cgatacgacc attggagggg
     4801 tcgcttctac ccccaggggc tgccgacgag ccgctggttg tccgcatacg ccgagcggtt
     4861 cgcgaccgtc gaggtgaaca acaccttcta ccacctgccc aaggccgaga cgttcgcgcg
     4921 ctgggcggtc cagacaccag cggggttcgt gtggagcgtc aaggccagcc ggtacgtcac
     4981 gcacatccgg cggctccggg actgcgggtc cgcggtggag ctcctgtggt cccgggcgat
     5041 cgagctcggc ccgcacctgg gtcccatcct cttccagctg ccgccgaccc tggccgccga
     5101 tcccgcgcgg ctggccgcgt tcctccgggt gctcccgacc ggcatgcgcg ccgccttcga
     5161 gttccgccac ccgagctggc tccgtcccga ggtgctctca ctgctggatg gagcgggtgc
     5221 cgcgctggtc ttggcggacc gcccgggagc ccgggtgcgg cccctggtga ccggcggctg
     5281 gtcctacgtc cgcctccacc aggggcgccc ggacgcgccc ggctatgcac ggcgcaagct
     5341 cgctcggtgg gccgagcgga tcgccgggct tccggcgagc gaggcgttcg tctacttcaa
     5401 caacgacacg ggtggcgccg cggtgcagga cgccctcacg atgcgggagc tgttggcagc
     5461 cagggggatc gagtggcctc gtagtccgcg atctgctcct cgtgacgaag ggtgagcccc
     5521 acgtcgtcca gcccttccag caagcgtcgt ctggtgaagt cgtccatctg gaacgcctga
     5581 tcgacaccgg gagcggtgac ccggagggct tccaggtcca ccgtgatcgg cgtggccgga
     5641 tcgcggtcca cggcctccat cagcgtgcgg accacgggtt cggggagccg aacggggagc
     5701 agtccgacct tcgtgcagtt ggtgcggaag atgtcgccga acgacggcgc caccacgatc
     5761 cggaacccgg cgtcctgcag cgcccagacc gcgtgttcac ggctcgagcc gcagccgaag
     5821 ttggggccgg ccaggaggat cgaggccccc cggtgttccg ggcggttgag gacgaagtcg
     5881 gggtcctcac gccacgcgcc gaacaggaag gggccgaacc cgctgcgctc gatccgcttg
     5941 aggtgctcgg ccgggatgat ctgatcggtg tcgacgtcgg cccggtccag ggggaccgcg
     6001 gtgccggtgt ggatgtggaa tggctccatc gccgttcagg ctcccttcgc ggtgcggagg
     6061 tcgataccca ggtcggccgg cgccgcgaag tggccggcga ccgccgtggc ggcggccacc
     6121 gcgggcgaga ccagatgggt acgaccaccc gcgccctggc gaccctcgaa gttccggttt
     6181 gaggtggagg cgctccgctc gccgggggcg agcttgtccg cgttcatcgc caggcacatc
     6241 gagcagccgg catcacgcca ctcgaagccg gcccgggtga agacggtgtc cagaccttca
     6301 ccctccgcct gcgccttcac cagcatcgac cccggaacca ccagggcccg gatcccctcg
     6361 tgcacccgac gcccccggac gacctgcgcg gcgatccgca ggtcctcgat ccggctgttc
     6421 gtgcacgagc cgatgaagac ggtgtccggc cggatctggc gcatcggggt cccgggggcg
     6481 aggtccatgt aggcgagggc tcgctccatc gtctcatcgg tgggctcggg aacgacagcc
     6541 tcgatggaga cggactggcc ggggttcgtc ccccagctca cgaagggcgt gaggtccgcg
     6601 gcgtcgatcg tcacctctcg gtcgaaggcg gcgtcggcgt cggtcgccag cgtccgccag
     6661 tggtcgagcg cgcgctccca ctcggccccc cgaggcgcac ccggccgacc ctccaggtac
     6721 gcgaacgtgg tctggtccgg cgcgaccagg ccggctcggg cccccgcctc gatcgacatg
     6781 ttgcacaccg tcatccgacc ctccatggac agggaacgga tcgccgagcc ccggtactcg
     6841 aggacgtggc ccacgccccc gcccgtaccg atccgcgcga tgatcccgag gatcaggtcc
     6901 ttggccgtga ccccgggcgg caggtcgccg tcgaccgtga cggccatcgg agcgggccgc
     6961 cgttgccgca gggtctgggt ggccagcacg tgctcgacct cgctggtgcc gatcccgaag
     7021 gccagcgccc cgaaggcgcc gtgggtggag gtatgggagt cgccgcacac gatcaccagg
     7081 cccggctggg tgagccccat ctggggtccg atcacgtgga cgatgccctg gccgatgtgg
     7141 ccggtgggga agagggtgat gccgaactcg gcgcagttgc ggcgcaaggc ctccagctgg
     7201 agcaccgaaa ccggatcctc cgggcccggg gccgtcggga cgttgtgatc catcgtggcc
     7261 acggtgaggt cc
//
LOCUS       DASQQQ010000114         7946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_202383, whole genome shotgun sequence.
ACCESSION   DASQQQ010000114 DASQQQ010000000
VERSION     DASQQQ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7946
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202383"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VE646_04770"
     CDS             complement(<1..435)
                     /locus_tag="VE646_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79337.1"
                     /translation="MTEATAPTGLRAESPAFIARPRQRAGIALTVLALILTIGAYALV
                     GLGKKGHLPTNLALYGSIFVVGYAGAYLTIRRTAPWADPAMFPVAGVLTGLGFAVIFR
                     LSGGLAAEQATWVVVGLIAFIATLLVVRDHRQLDAYTYTIGLV"
     gene            complement(432..1592)
                     /locus_tag="VE646_04775"
     CDS             complement(432..1592)
                     /locus_tag="VE646_04775"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HZD79338.1"
                     /translation="MRVRAGAATDIGMVRERNEDSFLVAEPLFVVADGMGGHLGGEVA
                     SRLTVETLERTSASLEDLVERVREANRVVFERSVSDRTVAGMGTTLTAAWVEGDRVRL
                     VHVGDSRAYLLRGGELRQLTEDHTLVQRMVLEGRITRQEAAVHPQRNVLTRVIGTDPV
                     VEPDDLELDLRPGDRVLLCTDGLTGMLGEEAIRDLLDSGLEPQLAAEELVREANHAGG
                     IDNTTVIVLDVEEGDPDSDPRSIVSSVQAPPPSTAARADTSPRGIDTIRGPQTRGRPR
                     WGRIALWTGVAVALVLAGLVGTRTYLDRQWYVGVSDGHVAVYRGIPSSVLGFDLHRVV
                     QETSIPAAEAERFPVYQDLEQGITTRDRDSALAIVQQIRTDVASIHRSGGSP"
     gene            complement(1589..2047)
                     /locus_tag="VE646_04780"
     CDS             complement(1589..2047)
                     /locus_tag="VE646_04780"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZD79339.1"
                     /translation="MSPFVLSVLKYSLLALLYFFIYRAVRAVVVDVAGRRPARGAEPR
                     RVVPTKATRGGRPPSSLVVHAPDGDKLGSFRLSEPLTVGRDPGCDVPLEDTYVSQEHA
                     RLYRQDGTWYVEDLGSTNGTYLNDRRVAAPSTVHAGDVVRVGKTVLEFRR"
     gene            complement(2049..2765)
                     /locus_tag="VE646_04785"
     CDS             complement(2049..2765)
                     /locus_tag="VE646_04785"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HZD79340.1"
                     /translation="MPILRDFERRLGNLVEGFFATTFRSGLQPVELAKRVLREMDAAR
                     TVGLNEVWAPNHFVFSLSQEDSQRFEQAEQALAHELKQVVRDAAAERGWGLVGPPEIV
                     FEVDEGLGRGKFRCRASLVEGEPEPPPARAADRPATVPGGSEASLTVTEEGAAARGYR
                     LGAALVTIGRLPECDVVLTDPAASRRHASIRAQGGGHLLTDLGSTNGTFVNDEPVGER
                     WLQDGDRITIGSAVLEFRRR"
     gene            2897..2981
                     /locus_tag="VE646_04790"
     tRNA            2897..2981
                     /locus_tag="VE646_04790"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2932..2934,aa:Leu,seq:cag)
     gene            3109..3651
                     /locus_tag="VE646_04795"
     CDS             3109..3651
                     /locus_tag="VE646_04795"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HZD79341.1"
                     /translation="MTRTPRVRLRLVGLAAALLLFAADCGGSASGESRTAACGNGQGT
                     AQQVPLRVLQGSSGATVALVPVSLDGKGPFAFALDTGAARSVVGRSVLNQLNVKTLGK
                     TKEPIAGIVGKVRATKVRITRWRAGEVKLPEPEIVLSIPLADQTGGGGPQGLLGSDIL
                     SSFGSITVDYQHQMLILCTS"
     gene            3738..4226
                     /locus_tag="VE646_04800"
     CDS             3738..4226
                     /locus_tag="VE646_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007258826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HZD79342.1"
                     /translation="MLDPTLEDALNDQMGREFFSSYLYLAMAAHFDQGNLPGMAAWMR
                     AQAEEEREHAMKFFDHVLDRGGSVRLAAIDAPPEKFGAPLDVFAQALGHEREITAAID
                     RLFAKADKPTEVFLQWFATEQVEEERSVEQIVEALRMAEDSGAALLMLDRELGSRGTA
                     EA"
     gene            4299..4757
                     /locus_tag="VE646_04805"
                     /pseudo
     CDS             4299..4757
                     /locus_tag="VE646_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018959703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
     gene            complement(5077..6468)
                     /locus_tag="VE646_04810"
     CDS             complement(5077..6468)
                     /locus_tag="VE646_04810"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HZD79343.1"
                     /translation="MRRLVAVGVFFELALLTIGLTWIRPMSDGQPARATTTSAASTDY
                     RIEGDGTGDVVEVNLVAENTVQEIAPGVKYQVWTFNGTAPGPVIRVHLGDTVRFTLTN
                     DDTMGMQHSIDFHAAQTPWDKNYVPVDPGKSLSFDWVARFPGVFMYHCGVPPVIQHIA
                     NGMYGAIIVEPDDLPTAREYVLVSGEFYPSKKPVNGAYVGDYDKMLEGTDPTYVVFNG
                     MADRYKDAPLEARPNELIRLYVVNAGPTLWNSFHVIGALFDHVYPSGNPTNQLNGLQS
                     WSVGPGDGAMYELRIPDAGLYPFVNHSFAYTGRGELGLLKIDPSAPAAPASYPAMGDP
                     FSAGVHPAGSASPTATAASGSTTSDRSTAAACEPKGTKLQIAAEGMAFDTDCLAAPAG
                     RRFTIAFDNRDEGVPHNLAIYTDADAGTALFVGDVITGPKATTYRVPGLDPGTYFFRC
                     DVHPQMNGTFVVA"
     gene            6591..7100
                     /locus_tag="VE646_04815"
     CDS             6591..7100
                     /locus_tag="VE646_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZD79344.1"
                     /translation="MNLTLSRRGDYVVRAAICLAGAWDGNGAYRKIREVADEMELPLS
                     YTPQVLGTLARAGLAEAKAGREGGYRLTRSPEQISLLEVIEAAEGYLVSERCPLRGGP
                     CRWDDTCAVHPTWIKASEAIRKVLSKTTLQEVSSVDRDLGSGAILDPAPKGHRVVDHA
                     HAGGPRRSA"
     gene            7201..7467
                     /locus_tag="VE646_04820"
     CDS             7201..7467
                     /locus_tag="VE646_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79345.1"
                     /translation="MGRWEGFEKCPGCGFDLMTGEGLRSCAWGDCPYLPEELDVFCPD
                     CRFNFFTMKGNPPCEDPMTCESGAEARMHIENLRHWAEQRELLS"
     gene            complement(7464..>7946)
                     /locus_tag="VE646_04825"
     CDS             complement(7464..>7946)
                     /locus_tag="VE646_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HZD79346.1"
                     /translation="VAFATRQPFIFPSLGPTAFLLFYTPQAAAASPRNTLAGHLIGCV
                     AGYVSLAIFGLLHAGPAIVVGVSGNRVGAAALSLALTAGFMAWLRVPHPPAGATTLIV
                     SLGLIATIPKLAVLMLGVLLLTIQGFAINRLAGVEYPVWAPKPPSRRTDVGGRPSSGA
                     "
BASE COUNT         1207 a         2746 c         2840 g         1153 t
ORIGIN      
        1 caccaggccg atcgtgtacg tgtacgcgtc cagctgccgg tggtcgcgca ccaccagcag
       61 ggtggcgatg aaggcgatga ggcccacgac gacccacgtg gcctgctccg ccgccagccc
      121 tccggagagc cggaagatca cggcgaagcc gagcccggtg aggacgccgg ccaccgggaa
      181 catcgccgga tcggcccacg gcgcggtgcg gcggatggtg aggtaagccc cggcgtagcc
      241 cacgacgaag atcgacccgt acagcgccag gttcgtgggc aggtggccct tcttccccag
      301 accgaccagc gcgtaggcgc cgatcgtgag gatcagcgca aggaccgtga gggcgatgcc
      361 cgcgcgctgt cgaggtctgg cgatgaaggc gggggactcg gccctcaggc cggtgggggc
      421 ggtcgcctcg gtcacgggct acctccgctc ctgtggatcg aggccacatc ggtgcggatc
      481 tgctgcacga tggccagcgc gctgtcgcga tcgcgggtgg tgatgccctg ctccaggtcc
      541 tggtagaccg ggaaccgttc cgcctcggcc gcggggatcg aggtctcctg gaccacgcgg
      601 tgcaggtcga accccaggac gctggagggg atcccgcggt acacggcgac gtgcccgtcg
      661 gagaccccca cgtaccactg tcgatccagg taggtgcggg tcccgacgag cccggccagg
      721 accagcgcca ccgcgacgcc ggtccacagc gcgatccgtc cccaccgagg gcggccccgt
      781 gtctgcggcc cccggatcgt gtcgataccc cggggcgagg tatccgctct cgccgccgtg
      841 gacgggggag gggcctggac ggacgagacg atggagcgag gatcggagtc ggggtcgccc
      901 tcctccacgt cgaggacgat caccgtggtg ttgtcgatgc cgccggcgtg attggcctcc
      961 cggaccagct cctcggcggc gagctgaggc tccaggcccg agtcgagcag atcgcggatc
     1021 gcctcctcgc ccagcatgcc ggtgagcccg tccgtgcaga gcagcacccg atcgccggga
     1081 cggaggtcca actccaggtc gtcgggctcg accacggggt cggtgccgat gacccgcgtc
     1141 agcacgttcc gttgcgggtg caccgccgcc tcctggcggg tgatccgacc ctccaggacc
     1201 atgcgttgga ccagggtgtg gtcctcggtc agctggcgga gctcccctcc gcggagcagg
     1261 taggcgcgcg agtcgcccac gtgcaccagg cggacccggt cgccctcgac ccaggcggcc
     1321 gtgagggtgg tccccatccc ggccacggtc cggtccgaga ccgagcgctc gaacaccact
     1381 cggttcgcct cgcgcacccg ctccaccagg tcctcgagcg aggcgctggt ccgctccagg
     1441 gtctccaccg tcaggcggga ggccacctcg ccgcccaggt gtccccccat cccgtcggcc
     1501 accacgaaca gcggctcggc caccaggaac gaatcctcgt tgcgctcccg caccatcccg
     1561 atgtccgtgg cggccccggc ccggaccctc atcggcggaa ctccaggacc gtcttgccca
     1621 cacgcacaac gtcgccggcg tggaccgtgg agggcgcggc gacacgcctg tcgttcaggt
     1681 acgtgccgtt ggtggacccc aggtcctcca cgtaccaggt cccgtcctgc cggtagaggc
     1741 gggcgtgctc ctgggagacg tacgtgtcct ccaacgggac gtcacaccct ggatcgcgac
     1801 ccacggtgag cggctcggag aggcggaagc tgccgagctt gtcgccgtcc ggcgcgtgga
     1861 ccaccagcga ggagggcggt cgtccgccgc gcgtggcctt ggtcggcacc acccggcggg
     1921 gctcggcgcc ccgtgccggt cgccggccgg cgacgtcgac caccaccgcc cggacggccc
     1981 ggtagatgaa gaagtacagg agggccagga gcgagtactt cagcaccgag agcacgaagg
     2041 gggacacgtc agcgcctccg gaactcgagc acggccgagc cgatcgtgat gcggtcccca
     2101 tcctgcagcc accgctcgcc caccggctca tcgttcacga acgtgccgtt ggtcgagcca
     2161 aggtccgtca gcaggtgccc tccgccttgg gcccggatcg aggcgtggcg gcgcgaggcg
     2221 gccgggtcgg tgaggaccac gtcgcactcg gggagccgtc cgatcgtgac gagcgcggcg
     2281 ccgagccggt atccgcgggc ggccgcgccc tcttcggtga ccgtgagcga ggcctcgctt
     2341 ccaccgggca ccgtcgcggg gcgatcggcc gctcgcgccg ggggtggctc gggctccccc
     2401 tccaccaacg acgcccgaca ccggaacttg cctcgcccca ggccctcgtc gacctcgaac
     2461 acgatctcgg gcgggccaac caggccccag ccacgctccg ccgccgcgtc gcgcaccacc
     2521 tgcttcagct cgtgcgccaa cgcctgctcc gcctgctcga accgctgcga atcctcctgg
     2581 gacagggaga acacgaagtg gttcggtgcc cacacctcgt tcaggccgac cgtcctggcc
     2641 gcgtccatct cgcggagcac ccgcttggcc agctccacgg gctgcaggcc gctgcggaac
     2701 gtggtggcga agaacccctc gaccaggttc cccagacggc gttcgaagtc acggaggata
     2761 ggcacgggtc ggatcgcgtt tgcgcaggtg gccgggcttt gcgcggatta tatcggcctt
     2821 ccggataccg gttggggagc ggtgcccggc ggcgtccagc actgctagca tctcggactg
     2881 cccacgtggg cgccacgggc gagtggcgga atttggcaga cgcgcaggat tcaggttcct
     2941 gtgagggcaa cctcgtgggg gttcaagtcc cccctcgccc accctctcgg atctccacca
     3001 gccaccccga ggacttcgtc cgtcccgagg gtgccgtccg ctatggccat cgtgtatcgg
     3061 ttcggaccgc cgggtatcca gcgggcaccg cgctctggag gaggaatcat gacccgcacc
     3121 ccccgcgtcc gtctccggct ggtgggactc gcagcggcgc tgctgctgtt cgcggcggac
     3181 tgcggcggat cggccagcgg cgagagcagg accgcggcct gcgggaacgg tcagggcacg
     3241 gcccagcagg tgcctctccg ggtcctccag ggcagctccg gtgccaccgt cgcgctggtc
     3301 ccggtgagcc tggacgggaa gggaccgttc gccttcgccc tggacacggg ggcggcccgg
     3361 tccgtggtcg gccggtccgt gttgaaccag ctgaacgtca agaccctggg caagaccaag
     3421 gagccgatcg ccggcatcgt cggcaaggtg agggcgacca aggtccggat cacgcggtgg
     3481 cgggcgggag aggtcaagct gcccgaaccc gagatcgtgc tctccatccc gcttgccgat
     3541 cagaccggtg gcggtggacc ccaaggcctc ctgggatcgg acatcctgag ctcgttcggc
     3601 tcgatcacgg tcgactacca gcaccagatg ctcatcctct gtacgagttg acgtccggga
     3661 tcgcgctccg cgcagccgca gcccgcatcg ccgcctgtcg gtacgatgca gggaaagcgc
     3721 ttcggaagga ggacgccatg ctcgacccca ccctcgagga cgccctgaac gaccagatgg
     3781 ggcgtgagtt cttctcgtcc tacctgtacc tggccatggc ggcgcacttc gaccagggga
     3841 accttcccgg catggccgcc tggatgcgtg cgcaggcgga ggaagagcgt gagcacgcca
     3901 tgaagttctt cgaccacgtg ctcgatcgtg gcggcagcgt gcggctggca gccatcgatg
     3961 cgccaccgga gaagttcggg gccccgctcg acgtgttcgc gcaggccctg gggcacgagc
     4021 gggagatcac ggccgccatc gaccggctgt tcgcgaaggc cgacaagccg accgaggtct
     4081 tcctccagtg gttcgccacc gaacaggtgg aggaggagcg ctcggtggag cagatcgtgg
     4141 aagcgttgcg catggccgag gacagcggtg ctgcgctcct gatgctggac cgcgagctcg
     4201 gttcccgcgg gaccgcggag gcgtagctct ccagccgcac cagctcgttc cggtgacccc
     4261 cgcgagcgcg ggaaccgcgc cgggcctatg ctcgcaaggt gttcgagcgg ttcacggatg
     4321 cggcccgcca tgccgtggtc ctggcccagg aggagtgcag gctcctgaac cacgaccaca
     4381 tcgggaccga gcacatcctg ctcgggctga tccgtgagca ggaggccatg ggggccaagg
     4441 cgctcgagtc gatgaacatc tccctggagg ccgtgcgcca gcaggtggag gagatcatcg
     4501 gtcagggcca ggccgcgccc accggccaca tcccgttcac gccgcgcgcc aagaaggtcc
     4561 tggagctgtc cctgcgcgag gcgctgcagc tcgggcacaa ctacatcggg accgagcaca
     4621 tcctgctcgg cctgattcgc gagggggagg gcgtggccgc ccaggtcctg cagaagctcg
     4681 gggccgatct gaaccgggtg cggcggaccg tgctggagct gctctcgggg tacgccgaag
     4741 ccgcctcctc gggcggcggg gccccggcgt cctccaccac gccggagcac gccggggcgg
     4801 agagggaggg ggggccagcg cccgaggtgg cagagccgtg gaacggaccg ctctgcccga
     4861 gctgcggagc ggagctcgcc accgcggccg cctaccgcag catcaccgtg ccggacgagc
     4921 aggcccgcaa cgggcgcccg atcgtcttcg tgttctgcgg gagctgctct cgcaccgtgg
     4981 gcatgctgcc cgccgactga tccggtccga tcggggacgt ggagaggggc cgggtgcgaa
     5041 acccggcccc tcggtggcgc gcggtggggg tccgagctac gccaccacga aggtcccgtt
     5101 catctgggga tggacgtcgc accggaagaa gtaggtgccg ggatccaggc ccggcacgcg
     5161 gtacgtcgtg gccttcggtc cggtgatcac gtcccccacg aacagggcgg tcccggcatc
     5221 cgcgtcggtg tagatggcca ggttgtgcgg gacgccctcg tcccggttgt cgaacgcgat
     5281 cgtgaaccgc cgcccggccg gagcggccag gcagtcggtg tcgaaggcca tcccctcggc
     5341 cgcgatctgc agcttcgtcc ccttcggttc gcaggcggcg gccgtcgacc ggtccgaggt
     5401 ggtcgagccg gaggccgcgg tggcggtggg gctcgcggac ccagcggggt ggacgccggc
     5461 ggagaagggg tcgcccatgg ccggatagga agccggggcg gcaggagcgc tggggtcgat
     5521 cttcaacagg ccgagctcgc cgcggcccgt gtaggcgaag gagtggttca cgaacgggta
     5581 gagcccggca tcggggatcc gcagctcgta catggccccg tcgcccggcc cgacgctcca
     5641 gctctgcagg ccgttcagct ggttcgtggg gttcccgctc ggatagacgt ggtcgaacag
     5701 tgcgccgatc acgtggaagg agttccagag ggtggggccg gcgttcacca cgtacaggcg
     5761 gatcaactcg ttcgggcggg cctcgagcgg ggcgtccttg taccggtcgg ccatcccgtt
     5821 gaacaccacg tacgtgggat cggtcccctc gagcatcttg tcgtagtcgc ccacgtacgc
     5881 accgttcacc ggcttcttgg acggatagaa ctcccccgag accagcacgt actcgcgggc
     5941 cgtcggcagg tcatcgggct ccacgatgat ggccccgtac atcccgttcg cgatgtgctg
     6001 gatcaccggc gggaccccgc agtggtacat gaacacaccg gggaagcgcg ccacccagtc
     6061 gaaggacagg ctcttcccgg ggtccacggg gacgtagttc ttgtcccacg gtgtttgggc
     6121 ggcgtggaaa tcgatcgagt gctgcatccc catcgtgtcg tcgttcgtga gcgtgaaccg
     6181 gaccgtatcc ccgaggtgga cacggatcac cgggccgggc gccgtcccgt tgaacgtcca
     6241 gacctggtac ttcacgccgg gcgcgatctc ctgcacggtg ttctcggcga cgaggttcac
     6301 ctccaccaca tcgccggtcc cgtcgccttc gatccggtag tcggtggacg cggctgaggt
     6361 cgtcgtcgcc cgcgcgggct ggccgtcgct catcggccgg atccaggtca gaccgatggt
     6421 gagcaaggcg agctcgaaga agacccccac cgccacgagc cgtctcatcg ttgcctcctc
     6481 ctccgtaccg cccggcgccg tcccgcagag cgaccttcct tctcccgcta aacgtggagt
     6541 atgatactac acgtatgtct ccgaggtaaa ggtccgatac actgggaggg atgaacctga
     6601 cgctctctcg acgcggcgac tacgtggtcc gcgccgccat ctgcctggcc ggcgcgtggg
     6661 atgggaacgg tgcctatcgg aagatccggg aggtcgccga cgagatggag ctgcccctca
     6721 gctacacgcc gcaggtcctc ggcacgctgg cccgggccgg cctggcggag gccaaggccg
     6781 gtcgggaggg cggctaccgc ctgacgcgtt cccccgagca gatctcgctc ctggaggtca
     6841 tcgaggcggc cgagggctac ctggtctcgg agcgctgccc cctgcgcggg ggcccctgcc
     6901 gctgggacga cacgtgcgcc gtccacccca cgtggatcaa ggcgtcggag gcgattcgca
     6961 aggtgctctc caagaccacg ttgcaggagg tgtcctccgt ggaccgggac ctggggagcg
     7021 gagcgatcct cgatccggcc ccgaagggcc atcgcgtggt ggaccacgcc cacgcgggcg
     7081 gtcctcgacg ctctgcctga gcctgttccc agatgggccg ttcgacaggg gtcggcaggg
     7141 gaccctcggc cctctcccgc gttcaggcct tcggggagca tggccgcgga ggcgatggac
     7201 atgggtcgat gggaaggctt cgagaagtgc cctggatgcg ggttcgacct catgacgggc
     7261 gaggggctcc gctcgtgcgc ctggggggac tgcccgtacc tcccggagga gctcgacgtg
     7321 ttctgcccgg actgccggtt caacttcttc acgatgaagg gcaaccctcc ctgtgaggat
     7381 ccgatgacgt gcgagagcgg cgcagaggcg cgcatgcaca tcgagaacct gcggcactgg
     7441 gccgagcagc gtgagctcct gagctaggcc cctgagctgg gacgaccacc gacatccgtc
     7501 cggcgggacg ggggcttggg cgcccacacc gggtactcga cgccggccag gcggttgatc
     7561 gcgaacccct ggatcgtgag cagcaggacc cccagcatca gcacggccag cttggggatc
     7621 gtggcgatca gcccgaggct cacgatgagc gtggtcgcac ctgcaggcgg gtgggggacg
     7681 cggagccacg ccatgaaccc cgcggtcaac gccagggaga gggccgccgc gcccacccgg
     7741 ttgcccgaga cccccaccac gatggccggg cccgcgtgga gcaggccgaa gatcgccagc
     7801 gagacgtacc cggccacgca gccgatcagg tggccggcca gggtgttgcg gggcgaggcc
     7861 gccgcggcct ggggcgtgta gaagagcagg aaggcggtgg gtccgagcga cgggaagatg
     7921 aagggctgcc gggtggcgaa cgccac
//
LOCUS       DASQQQ010000115        11175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_202482, whole genome shotgun sequence.
ACCESSION   DASQQQ010000115 DASQQQ010000000
VERSION     DASQQQ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11175
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202482"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..328)
                     /locus_tag="VE646_04830"
     CDS             complement(5..328)
                     /locus_tag="VE646_04830"
                     /inference="COORDINATES: protein motif:HMM:NF015302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HZD79347.1"
                     /translation="MSPRGVAVALLIGTGTFVAVVCSLGLLVLPDIFDRLHFLSGLTV
                     ATLLVTTGVVVRTGWHAISASAAIVAAGALVAGPTLTHAVGRTARFRDHGTLEIDDGE
                     RSGPP"
     gene            complement(325..576)
                     /locus_tag="VE646_04835"
     CDS             complement(325..576)
                     /locus_tag="VE646_04835"
                     /inference="COORDINATES: protein motif:HMM:NF015993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HZD79348.1"
                     /translation="MTGPLVAASVLLVCFLPCGALVLRGRREDAVIGVTASGVLGQLV
                     LLLLAAAMDRSIYSEAAVALVLLSFGGSLIFLRFLERWA"
     gene            complement(573..1061)
                     /locus_tag="VE646_04840"
     CDS             complement(573..1061)
                     /locus_tag="VE646_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79349.1"
                     /translation="MSWRPSLIARAVTGTVLGFALWLGLVVSTDLQELLAGVIACTAV
                     AAVAPLLRSRPARTSLGWVHVVRPVLHSLPRLVPDTVALTVILWRRATRGAEPPSRFV
                     AVRAPVGGHDARAVGRRVAATVGASLAPGAYVVHTTEDGWILLHQLRPASSTGIRGAS
                     QP"
     gene            1171..3444
                     /locus_tag="VE646_04845"
     CDS             1171..3444
                     /locus_tag="VE646_04845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZD79350.1"
                     /translation="MLLSLFLVCFVSLVLSTILLTGVGRRLRLSARPLGIAGAALSTV
                     ASGAIVAALWHPPAGHVRVGEALLFVVTAMVVLLRPRWNPSGQVFFGALLASALSYLA
                     FAGEVTFASGLSILGSAASALLLVLELIALTLSASFAFESLDVICRTRWDRRDVPWDP
                     AYLPMVSLQIAAYNEPPDMLIETIRSVEGLDYPNFELVVIDNNTKDPEVWGPVEDYCR
                     GRPRVRFIHVDPWPGYKSGALNLALTAYTDPNAELVGVIDADYLVDPGYLRRVVGYFS
                     DPRLAFLQTPQDYREYEGDAYLTACYDAYRYFFLTTMPSRNERDSIIFAGTMGLLRRS
                     VLEELGGWDEWCITEDAEASLRMLKAGYSGLFIGRSFGRGIMPLSFASLKSQRFRWCF
                     GGMQILRSHWRELMPWNRDPRNHLSLAQRIDYLLGGLQWLNDLVYLGFTLVLLATAIL
                     LATRGWFALRPLIGLAVLLPAALIGSGLLRAIWALRARAGIGMRRAVLAFLNWLSLSW
                     TVALACVQGLTRAKGVFLRTPKAGDRSGVLAAMWTARTETFLALVVWGAGALAVIRGH
                     ATWFVLGLFAWQGAVYAAAPYMSWLNQHTELSAQLERRRRSEWRRERLATAGPYAIGA
                     LSGAAIGAGIVALILLGGTQAAPPASSPFTVPHAATGQGSSASGGTALPSPSQSPSPT
                     TSPTPTVTPTPTPTPTPTPTATPSVTPSPSPSPETSPSPSAVTSPSSSPVASLTPLPS
                     ASVAATGGPAAAPIAGP"
     gene            3441..4559
                     /locus_tag="VE646_04850"
     CDS             3441..4559
                     /locus_tag="VE646_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HZD79351.1"
                     /translation="MRGVRMAGARRWWLILGAVLAGAAVVCAVVVVRGAEGGEPGVER
                     QARTAGERFLDRYVDEDGRVVRRDQGDDTVSEGQAYAMLVAVALGDQARFSRVWGWTT
                     HHLQRPDGLLSWHWQDGAVVDPQSAADADVETARALLLAAERFGQPAYRRAGERIAGA
                     ILRVETVTVGSQRVLMAGPWARSLPAWIDPSYFIPGAFRLLYRTTGDARWRAVNRSSL
                     SLLEHLTEPIPHLPPDWARLSFQGLAIPAPAPAGGGMAEFGLDAVRVPVQMAEDCRPA
                     GRRIAASLWPFFRQAATSGIVTRYDLQGHPLTQDRHPTGWVAAAAAAEAAGHQAWALS
                     ALDHAQRMEAQHSTYYGAAWVALGRILLTTRWLGGCGS"
     gene            complement(4659..5390)
                     /locus_tag="VE646_04855"
     CDS             complement(4659..5390)
                     /locus_tag="VE646_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR1/FUN34/YaaH family transporter"
                     /protein_id="HZD79352.1"
                     /translation="MAEPAQIRPTSEQQVAGEVQPRVVLQPLAAPSILGLFGFAGATL
                     IVAANMAGWYGNSTTTAFFLAPFALAFGGIAQFLAGMWAFKARDGLATAMHGMWGSFW
                     IGYGILWLLVGTGVLPTSGLDTAFGFWFIPLAAITYSGAFAALSDGNLGVFSVLLLLA
                     TGAAFAAGALMSGGHFWEIVAGWLFVASAVDAWYVATAMMLEGAAGKVILPLFKMRKD
                     TNIPGRKPTRPIQLEWAEPGIKRGQ"
     gene            5584..8862
                     /gene="fdh"
                     /locus_tag="VE646_04860"
     CDS             5584..8862
                     /gene="fdh"
                     /locus_tag="VE646_04860"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:6160..6162,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HZD79353.1"
                     /translation="MSIEDRDGRSTTWPVLRQLVQRDPGGLGKSAFSKRTIELHPRTR
                     DADEVVKSICPYCGVGCGQRVFVKDGRITDIEGDPDSPISEGCLCPKGAASFQLVTGS
                     QREHQVLYRRPGGAEWEPISLDRAMDMVAERVKRTRDETWQDENENGHPLHRTLGVAH
                     LGGATLDNEENYLIKKLFTALGVIQVENQARIUHSSTVPGLGTSFGRGGATSFQQDLQ
                     NADCIVIEGSNMAECHPVGFRWVMEARRKGAKLIHVDPRFTRTSAMADIHVPIRAGTD
                     IAFLGGIVNHIIQNEKYFRDYVVNYTNAASIISDEFQDTEDLDGLFSGWSHERGQYGP
                     ESWQYRGIEVAPAAGQREMFTGEAHSERGTRIRAIEHDFTLQDPRCVFQIVKRHYQRY
                     TPEVVEDICGIPKEQFLQVAETLCENSGRERTSAFCYAVGWTQHSIGVQYIRTAAIVQ
                     LLLGNIGRPGGGIMALRGHASIQGSTDIPTLYNILPGYLPMPKASFDISLKDYIENNE
                     SAAGWWSEFPKYIVSLLKAYWGDAATEDNDFCYGYLPHLSGDHSHMTTVADMADGLVK
                     GYFVMGENPTVGSMHGAFHREGLRRAEWVVVRDFAPTETAEFWKVAPEIDRGEVKPED
                     IQTEVFFFPAAAHVEKDGAFTQTQRLLQWHHKAVEPPGDCRSELHFMYHLGKRLKALY
                     ADSAEGKDRPILDLTWDYPTQGPHEEPDAEMVLKEINGFTVDDGKPVDGFTSLKADGS
                     TACGCWIYSGAYKDGVNQTARRKPAGEQHWVAPEWGWAWPSNRRILYNRASADPEGRP
                     WSERKRYVWWDEEQGQWTGHDVPDFIKDRAPSYRPDDDATGTDTLAGIDPFIMQADGK
                     GWLYAPNGLLDGPLPTHYEPQESVVENALYGQQCNPARMEWDRRENPYHRPWGDPRFP
                     YILTTYRLTEHHTAGGMSRWLSWLSELQPEMFCEVSPQLADEVGLRNGDWATISTARA
                     EIEARVLVTPRIRPIKVRGRVFHQIGLPYHWAGKGLVTGDPTNELISFVADPNVSIQE
                     SKALTGNIVPGRRSRGRRVVTSGPLEPTPPDGSRARDLPIAQDKPEGRHGVQAGESRE
                     GAQT"
     gene            8862..9698
                     /locus_tag="VE646_04865"
     CDS             8862..9698
                     /locus_tag="VE646_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZD79354.1"
                     /translation="MAVKGFFTDTTVCIGCKACEVACKEWNQLPDDGMVFTGMSLDNT
                     VDLGASTWRHVSFMERPGVSLPTSDVFGAQTPAAFGSQTSPDGQFSWLMMSDVCKHCQ
                     RAGCLENCPTGAIVRTEFDSVYVQPDVCNGCGYCIVGCPFGVIDRRADDGRAWKCTLC
                     YDRLQDDMTPACAQACPTDSIQFGDLTELHAKAEERVQTLHERGMADAYLYGAGEENQ
                     PGTGGLNAFFLLVDQPEVYNLPPDPVVPTKKAGQAWRSVAGAAAAMVGAALVAVLTSG
                     GE"
     gene            9698..10939
                     /gene="nrfD"
                     /locus_tag="VE646_04870"
     CDS             9698..10939
                     /gene="nrfD"
                     /locus_tag="VE646_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HZD79355.1"
                     /translation="MDARHDQQPERNAGDGRNIDPATGILEGEASEQVVPTRRAFIEG
                     SPEFDVWTQGPPIAADGKRAAEPTYYDRPAIKEPVWIWAVPAYFYAGGAAGAAAALGA
                     VAQGIDRRGMATLIERCRWVAGIGGGIGTGLLIYDLGRPERFLNMLRVFRPTSPLNLG
                     SWILAGATPASSGAAALSHADGLLGALGDVGGMVGGSLGVPLAGYTAVLLSNTSVPLW
                     QETRRTLPALFYASAVSSAACLLDLFHLNRREIAVVNRFGNAGKAAELALAEALERDA
                     FRIPEVGAPLRHGVSGALWRGAKALTGASLAVSSAPGTWRWKRALSGVLGTAGAIALR
                     FALFQAGKVSARDPRTTFRQQRQGHGAEEVTGQSAIAGPRGIRATSQESVVAQPANDG
                     DRMPLPDLEQAAGKEPGTGSM"
     gene            complement(10976..>11175)
                     /locus_tag="VE646_04875"
     CDS             complement(10976..>11175)
                     /locus_tag="VE646_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79356.1"
                     /translation="ELDVSEITDGHAADVLRGIFISLDLEKPTPDGTVQFYPPGSPPW
                     SSWVLSTILPQRSMLRADIEA"
BASE COUNT         1715 a         3888 c         3936 g         1636 t
ORIGIN      
        1 acgttcacgg tggacccgac cgctcgccgt cgtcgatctc cagcgttccg tgatcccgga
       61 accgggccgt ccgcccgacg gcatgcgtga gggtcgggcc ggccaccagc gcgccggcgg
      121 caacgatggc cgcgctggcc gagatcgcgt gccacccggt acgcaccacg acccccgtgg
      181 tcaccagcag ggtggccacg gtcagtccgg agaggaagtg gagccggtcg aagatgtcgg
      241 gcaggaccag caggcccagg gagcacacga ccgccacgaa ggtccccgtg ccgatcagca
      301 gggccacggc cacaccccga gggctcatgc ccatcgctcc aggaaccgca ggaagatgag
      361 cgatcctccg aacgacagca ggacgagggc caccgccgcc tccgagtaga tcgagcggtc
      421 catcgccgcg gccagcagca gcagcaccag ctggccgagg acccccgacg cggtgacccc
      481 gatcacggcg tcctctcgcc tcccacgcag caccagggcc ccgcagggga ggaagcagac
      541 caggagcacc gaggcggcca cgagcggtcc ggtcatggct gagaggcgcc ccggatcccc
      601 gtgctcgagg cagggcgcag ctggtggagc aggatccagc cgtcctcggt cgtgtgcacc
      661 acgtacgccc cgggggcgag cgacgcccca acggtcgccg ccacgcggcg gcccacggcg
      721 cgtgcgtcgt gtccgcccac gggggcacgg acggccacga agcggctggg gggctcggct
      781 ccgcgagtcg ctcggcgcca caggatcacg gtcagcgcca cggtgtccgg cacgagccgt
      841 ggcaggctgt gcaggacggg ccgaacgaca tggacccatc ccaggctggt cctggccggg
      901 cgggagcgca gcagcggtgc gaccgctgcc accgcggtgc aggcgatcac acccgccagg
      961 agctcctgca gatccgtgga gaccaccagg cccagccaca gggcgaagcc gagcacggtc
     1021 ccggtgaccg ctcgggcgat gagcgatgga cgccaactca tccccatctc gcactccttt
     1081 ccggggcagc tattcccggt ggctgcaggg tcaaaccgac accctccgcc tctccgtttc
     1141 ctgtatcccc caggtgggga tgtggcaccc gtgcttctca gcctgttcct cgtctgcttc
     1201 gtgagcctgg tgctgagcac catcctcctc acgggggtgg ggcgccggct gcgtctgtcc
     1261 gcacgtccgc tgggcatcgc gggggccgcc ctctcgaccg tcgcctccgg tgcgatcgtg
     1321 gcggcgctct ggcacccccc cgccggccac gtccgggtgg gggaagcgct cctgttcgtg
     1381 gtcacggcga tggtggtgct gctgcggcct cggtggaacc cgtcggggca ggtgttcttc
     1441 ggcgcgctgc tcgcctccgc cctctcgtac ctggcgttcg cgggcgaggt gacgttcgcc
     1501 agcggcctct cgatcctggg ctcggccgca tcggcgctgc tgctggtgct cgagctgatc
     1561 gccttgacgc tttccgcatc gttcgcgttc gagagcctgg acgtcatctg ccggacgcgg
     1621 tgggatcggc gagacgtgcc ctgggatccg gcgtacctgc ccatggtgtc cctgcagatc
     1681 gccgcgtaca acgaaccgcc ggacatgctg atcgagacga tccggtcggt ggagggactc
     1741 gactacccga acttcgagct ggtcgtgatc gacaacaaca cgaaggaccc ggaggtctgg
     1801 ggaccggtcg aggactactg ccgcggccgg cctcgagtgc ggttcatcca cgtcgatccg
     1861 tggcccggct acaagtccgg tgccctgaac ctcgccctca ccgcctacac cgatccgaac
     1921 gccgagctgg tcggcgtgat cgacgccgac tacctggtcg atcccggtta cctccgacgg
     1981 gtcgtgggct acttctccga tccacggctg gccttcctcc agacgcccca ggactaccgg
     2041 gagtacgagg gcgacgcgta cctgaccgcc tgctacgacg cgtaccggta cttcttcctg
     2101 acgacgatgc cgtcgcgcaa cgagcgggac tcgatcatct tcgccggcac catggggctg
     2161 ctccgtcgct cggtgctcga ggagctcgga ggctgggacg agtggtgcat caccgaggat
     2221 gccgaggcct cgctgcgcat gctgaaggcc ggctactccg ggttgttcat cgggaggtcg
     2281 ttcggacggg gcatcatgcc gctgtccttc gcgtcgctca agagccagcg gttccgttgg
     2341 tgcttcggcg ggatgcagat cctgcgcagc cactggcggg agctcatgcc ctggaaccgg
     2401 gacccccgca accacctcag cctggctcag cggatcgact acctgctggg cgggctgcag
     2461 tggctgaacg acctcgtgta cctgggcttc acactggtgc tgctggccac cgccatcctg
     2521 ctcgccaccc gcggctggtt cgccctccgg ccgctgatcg gcctggccgt gctgttgccg
     2581 gccgcgctga tcggatcggg tctgcttcga gcgatctggg ctctccgagc cagggccggc
     2641 atcggcatgc gccgagccgt cctggccttc ctgaactggc tctcgctgtc ctggacggtc
     2701 gcgctcgcct gcgtgcaggg gctcacccgg gcgaagggag tcttcctgcg cacgcccaag
     2761 gcgggggacc ggagcggtgt gttggcggcc atgtggaccg cgcgcaccga gacgttcctc
     2821 gccctcgtcg tgtggggagc gggagcgttg gccgtgatcc gcggacatgc cacctggttc
     2881 gtcctcggac tgttcgcgtg gcagggtgcc gtctacgccg cggctcccta catgtcgtgg
     2941 ctcaaccagc acaccgagct ctccgcccag ctcgagcggc gtcgtcgctc cgagtggcgg
     3001 cgggagcggc tggccacggc cgggccgtac gcgatcggag cgctctcggg agccgcgatc
     3061 ggcgcaggca tcgtggccct catcctgctc ggtgggaccc aagcggcccc tccggcctcg
     3121 agccccttca cggtgccgca tgcggccacg ggccagggga gctcggcgag cggtggcacg
     3181 gctctcccgt cgccgagcca gagtcccagt cccaccacgt ctcccacccc gaccgtcacg
     3241 ccgacgccga cgccgacgcc gacgccgacc ccgaccgcga cgccgagcgt gacgccgagt
     3301 ccgagcccga gcccggaaac gagcccttcg ccctcggccg tgacgagccc gagcagctcg
     3361 cccgtcgcct cgctgacgcc gctgccgtcg gcgtcggtcg ccgcgacggg cggcccggcg
     3421 gccgcaccga tcgcggggcc gtgaggggcg tccgcatggc cggggccaga cgctggtggc
     3481 tgatcctcgg agcggtgctc gccggcgcgg ccgtggtgtg cgccgtggtc gtggtccgcg
     3541 gggcggaggg gggcgagccc ggcgtggagc ggcaagctcg gaccgccggg gagcggttcc
     3601 tcgaccgcta cgtcgatgag gatgggcgcg tggttcgccg ggaccaagga gacgacacgg
     3661 tgagcgaggg gcaggcgtac gcgatgctgg tggccgtggc cctcggcgac caggcccgtt
     3721 tctcccgggt ctggggttgg accacgcacc acctccagcg ccccgatggc ctgttgtcct
     3781 ggcattggca ggacggtgcc gtggtggatc cccagtcggc ggcggacgcc gacgtcgaga
     3841 cggcgcgagc gctgctgctc gcggccgagc gtttcgggca gcccgcctac cgccgagccg
     3901 gtgaacggat cgccggcgcg atcctccgcg tggagaccgt gacggtgggc tcgcaacggg
     3961 tcctgatggc gggtccctgg gcccgttccc tcccggcctg gatcgatccc agctacttca
     4021 tccccggcgc cttccgcctc ctgtaccgga ccaccgggga cgcgcgctgg cgggccgtga
     4081 accgatcctc gttgagcctg ctggaacacc tcacggagcc gatcccccat ttgcctccag
     4141 actgggctcg cctctccttc caggggctcg ccatccccgc ccctgcaccg gctggaggcg
     4201 gcatggccga gttcggcctc gacgccgtcc gcgtcccggt gcagatggcg gaggactgcc
     4261 gcccggctgg ccggcggatc gccgcatcgc tctggccctt cttccgccag gcagcgacct
     4321 cggggatcgt cacccggtac gacctgcagg ggcatcccct gacccaggac cgccacccga
     4381 cgggctgggt ggccgcggcc gccgcagccg aggccgcggg ccaccaagcc tgggccctgt
     4441 cggcgctgga tcacgcccaa cggatggagg cgcagcactc gacgtactac ggagccgcct
     4501 gggtcgctct gggccgcatc ctgctcacca ctcggtggtt gggggggtgc ggatcctgag
     4561 cgggtcgaga gccggacggg gtggcggaca tggaagaagg ccccgcggtc gcggggcctt
     4621 ctcggttcct tcttcctcgg tcggaagccg ttgcgggatc actgaccccg cttgatgccc
     4681 ggctcagccc actcgagctg gatggggcgg gtcggcttgc gtccggggat gttggtgtcc
     4741 ttccgcatct tgaacagcgg aaggatgacc ttgcccgcag cgccctcgag catcatggcc
     4801 gtcgccacgt accaggcgtc gaccgccgag gccacgaaca gccagcccgc gacgatctcc
     4861 cagaagtggc cgccggacat gagggctccg gcggcgaacg cagcacccgt cgccagcagg
     4921 agcagcaccg agaagacacc gaggttcccg tcgctgagcg cggcgaacgc gcccgagtag
     4981 gtgatcgcgg ccagcgggat gaaccagaac ccgaaggccg tgtccaaccc actcgtgggc
     5041 aacacaccgg tgccgacgag cagccacaag atcccgtagc cgatccagaa ggatccccac
     5101 atcccgtgca tggccgtagc caggccgtcc cgggccttga aggcccacat cccggccagg
     5161 aactgggcga tccctccgaa cgccaatgcg aacggggcca ggaagaacgc ggtcgtcgtg
     5221 gagttgccgt accaaccggc catgttcgcg gcgacgatca gggtggcgcc tgcgaacccg
     5281 aacagcccca ggatcgatgg ggctgcgaga ggctgcagca ccacacgtgg ctggacctcg
     5341 ccagccacct gttgctcgga cgtcgggcgg atctgtgcag gttccgccat cagacgtcac
     5401 ctcctttcgc tcacgcccct acagataccc atcgcttctc catctcatcg cggatccggg
     5461 gatcggatac cgacacctct ggacgcgacg acggtccgcg ggacctgtcg tttgccctgg
     5521 gagcgacgcg ggtacgcgcc agggtgcagc cgtcccacag ccatcccgac gatggaggtc
     5581 cgtatgagca tcgaggaccg tgatggtcgg tccacgacgt ggccggtctt gaggcagttg
     5641 gtgcagcggg atccgggagg gctcggcaag agcgccttct ccaagcggac catcgagctg
     5701 cacccgcgga cccgggatgc ggacgaggtg gtgaagtcca tctgtcccta ctgcggcgtc
     5761 gggtgtgggc agcgggtctt cgtgaaggac ggccggatca ccgacatcga gggcgacccg
     5821 gactccccga tctcggaagg gtgcctgtgc cccaagggcg cggcgagctt ccagctggtc
     5881 accgggtccc agcgcgagca ccaggtgctg taccgacggc cggggggggc cgagtgggag
     5941 ccgatctcgc tcgaccgggc catggacatg gtggccgagc gcgtcaagcg gacccgcgat
     6001 gagacctggc aggacgagaa cgagaacggc catccgctcc accgcacgct cggcgtcgcg
     6061 cacctgggtg gagcgacgct ggacaacgag gagaactacc tcatcaagaa gctgttcacg
     6121 gccctgggcg tgatccaggt cgagaaccag gcccgcatat gacacagctc cacggtcccc
     6181 ggtctgggga cctcgttcgg acggggcggc gccacgtcct tccagcagga cctgcagaat
     6241 gccgactgca tcgtgatcga gggctcgaac atggccgagt gtcacccggt gggcttccgc
     6301 tgggtgatgg aggcccggcg caagggcgcc aagctgatcc acgtggaccc gcggttcacg
     6361 cgcaccagcg cgatggccga catccacgtc ccgatccggg ccggcaccga catcgccttc
     6421 ctgggcggga tcgtgaacca catcatccag aacgagaagt acttccgcga ctacgtcgtg
     6481 aactacacga acgcggcatc gatcatctcc gatgagttcc aggacaccga ggacctggac
     6541 ggcctgttca gcggctggag ccacgagcgc ggccagtacg gccccgagag ctggcagtac
     6601 cgggggatcg aggtggcgcc cgccgccggg cagcgagaga tgttcaccgg cgaggcgcac
     6661 tcggagcgcg ggacccggat ccgcgcgatc gagcacgact tcaccctgca ggacccgcgc
     6721 tgcgtcttcc agatcgtgaa gcgccactat cagcgctaca cgcccgaggt ggtcgaggac
     6781 atctgcggca tccccaagga gcagtttctc caggtggccg agacgctatg cgagaactcg
     6841 gggcgggagc ggacctccgc gttctgctac gcagtgggtt ggacgcagca ctccatcggc
     6901 gtgcagtaca tccggacggc ggccatcgtc cagctgctgc tggggaacat cggccggccg
     6961 ggcggcggga tcatggccct gcgcgggcac gcctcgatcc agggcagcac cgacatcccg
     7021 accctgtaca acatcctgcc gggctacctg ccgatgccga aggcgagctt cgacatctcg
     7081 ctgaaggact acatcgagaa caacgagtcg gcggccggct ggtggagcga gttccccaag
     7141 tacatcgtct cgttgctcaa ggcgtactgg ggcgacgcgg ccaccgagga caacgacttc
     7201 tgctacgggt acctgccgca cctgtccggc gaccactcgc acatgaccac ggtggccgac
     7261 atggccgatg gtctggtgaa gggctacttc gtgatggggg agaaccccac cgtgggctcg
     7321 atgcacggcg cgttccaccg cgagggcctc cgccgggccg agtgggtggt ggtccgggac
     7381 ttcgccccca ccgagaccgc cgagttctgg aaggtggcgc ccgagatcga ccgcggcgag
     7441 gtcaagccgg aggacatcca gaccgaggtg ttcttctttc ccgccgctgc ccatgtggag
     7501 aaggacggag ccttcaccca gacccagcgc ctgctgcagt ggcaccacaa ggcggtggag
     7561 ccaccgggcg attgccggag cgagctgcac ttcatgtacc acctgggcaa gcggctgaag
     7621 gcgctctatg cggattccgc cgagggcaag gaccggccga tcctcgatct cacctgggac
     7681 tacccgacgc agggaccgca cgaggagccc gacgccgaga tggtgctgaa ggagatcaac
     7741 gggttcaccg tggacgacgg gaagccggtc gacgggttca cctcgctgaa agccgacggc
     7801 tcgaccgcct gcggctgctg gatctactcc ggtgcctaca aggacggcgt gaaccagacc
     7861 gctcggcgga agccggccgg cgagcagcac tgggtggccc ccgagtgggg gtgggcctgg
     7921 ccgagcaacc ggcggatcct ctacaaccgg gcctcggccg atcccgaggg acgcccctgg
     7981 tctgagcgga agcgctacgt gtggtgggac gaggagcagg gtcagtggac cggccacgac
     8041 gtccccgact tcatcaagga ccgtgcgccc tcgtaccggc ctgacgacga cgcgacgggc
     8101 acggacaccc tggccgggat cgaccccttc atcatgcagg ccgacggcaa ggggtggttg
     8161 tacgcgccga acggcctgct cgatggcccg ctgcccacgc attacgagcc gcaggagtcg
     8221 gtggtcgaga acgccctgta cggtcagcag tgcaatcccg cccggatgga gtgggaccgg
     8281 agggagaacc cctaccaccg tccgtggggc gaccctcggt tcccgtacat cctgaccacc
     8341 taccggctga ccgagcacca cacagccggg gggatgtccc ggtggctgtc ctggctctcc
     8401 gagctccagc cggagatgtt ctgcgaggtg tcgccgcagc tcgccgacga ggtgggtctg
     8461 cgcaacggcg actgggccac gatctccacc gccagggccg agatcgaggc ccgggtcctg
     8521 gtgaccccca ggatccggcc catcaaggtg cggggccgcg tgttccacca gatcggcctg
     8581 ccctaccact gggcgggcaa ggggctggtg accggcgatc ccacgaacga gctgatctcg
     8641 ttcgtggccg accccaacgt ctcgatccag gagtccaagg ccctgaccgg gaacatcgtg
     8701 ccggggcgtc ggagccgggg ccgtcgggtg gtcaccagcg gcccgctcga gccgacgcct
     8761 ccggacggct cccgggcccg ggacctcccc atcgcccagg acaaaccgga gggccgtcac
     8821 ggcgtccagg cgggggagtc gcgagagggg gcgcagacct gatggcggtc aagggcttct
     8881 tcaccgatac cacggtctgc atcggctgca aggcgtgcga ggtggcctgc aaggagtgga
     8941 accagctccc ggacgacggg atggtcttca cgggcatgtc cctcgacaac acggtcgacc
     9001 tcggcgcctc gacctggcgg cacgtctcgt tcatggagcg accgggggtc tcgctgccga
     9061 cctcggacgt cttcggggcg cagacgccag cggcgttcgg ctcgcagacc tctcccgacg
     9121 gtcagttctc ctggctgatg atgtccgacg tctgcaagca ctgccagcga gcgggctgcc
     9181 ttgagaactg ccccaccggt gccatcgtgc gcaccgagtt cgactcggtc tacgtgcagc
     9241 ccgacgtctg caacgggtgc ggctactgca tcgtggggtg cccgttcggc gtgatcgatc
     9301 ggcgcgccga cgacggccgg gcctggaagt gcaccttgtg ctacgaccgg ctccaggacg
     9361 acatgacccc ggcctgcgcg caggcctgtc ccacggactc gatccagttc ggcgacctca
     9421 ccgagctcca cgccaaggcg gaggagcggg tgcagaccct gcacgagcgg ggcatggccg
     9481 acgcctacct ctacggggcc ggcgaggaga accagcccgg cacgggcggc ctgaacgcct
     9541 tcttcctcct ggtggaccag ccggaggtct acaacctgcc tcccgacccg gtggtgccca
     9601 cgaagaaggc ggggcaagcc tggcggtcgg tggccggcgc ggcggcggcc atggtcggcg
     9661 ctgccctggt ggcagtgctc acgagcggag gcgagtgatg gacgcacggc acgatcagca
     9721 gcccgagcgc aacgccggcg acgggcgcaa catcgacccg gccaccggca tcctggaggg
     9781 ggaggcgtct gagcaggtcg tccccacccg gcgcgccttc atcgaagggt ccccggagtt
     9841 cgacgtctgg acccaagggc caccgatcgc cgcggacggg aagcgcgccg ccgagcccac
     9901 gtactacgac cgacccgcga tcaaggagcc ggtgtggatc tgggcggtcc cggcctactt
     9961 ctacgcgggc ggggcggcgg gtgcggccgc cgcgctcgga gcggtggccc agggcatcga
    10021 ccggcggggc atggccacgc tcatcgagcg atgccgatgg gtggccggga tcggtggagg
    10081 gatcgggacc ggtctcctga tctacgacct gggtcgtccc gaacggttcc tgaacatgct
    10141 gcgcgtcttc cgcccgacgt ccccgctgaa cctcggttcg tggatcctgg ccggggccac
    10201 gcccgcgtcc tcgggtgcgg cggcgctgag tcacgccgac gggctgctgg gggcgcttgg
    10261 cgacgtgggc gggatggtcg gtggttcgct cggcgtgccg ctggccgggt acacggcggt
    10321 cctgctctcg aacacgtcgg tgccgctgtg gcaggagacc cgtcgcaccc tgcccgccct
    10381 gttctacgcg agcgcggtgt ccagcgcggc atgcctgctc gacctgttcc acctgaaccg
    10441 tcgggagatc gccgtggtga acaggttcgg caacgcgggg aaagcggcgg agctggcact
    10501 ggccgaggcg ctggagcggg acgcgttccg catccccgag gtgggagccc cgctccgaca
    10561 cggcgtgagc ggcgcgctct ggcggggcgc gaaggcactg accggggcga gcctggcggt
    10621 ctcctccgcc ccgggcacct ggcgatggaa gcgggcgctg tccggggtgc tggggacggc
    10681 cggcgccatc gccctgcggt tcgcgctgtt ccaggcgggg aaagtctcgg cgcgtgaccc
    10741 acggaccacg ttccgtcagc agcgccaggg acatggtgcc gaagaggtca ccgggcagag
    10801 tgccatcgca ggtccccgcg ggatccgagc cacctcacag gagagcgtgg tggcgcagcc
    10861 cgctaacgac ggggatcgga tgcccctccc cgatctggag caagcggccg ggaaggagcc
    10921 ggggaccggc tcgatgtaga tcgcaccggg aggtgccggg aggtgccggc ttcccttacg
    10981 cctcgatatc tgctcgcagc atgctccgtt gcgggaggat cgtgctgagc acccagctgg
    11041 accatggggg actgccgggc gggtagaact gcaccgtgcc gtcaggggtc ggcttctcca
    11101 gatccaggct gatgaagatc ccacgcagca cgtccgccgc atgaccatcc gtgatctcgg
    11161 aaacgtcgag ctccc
//
LOCUS       DASQQQ010000116        13931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_146160, whole genome shotgun sequence.
ACCESSION   DASQQQ010000116 DASQQQ010000000
VERSION     DASQQQ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13931
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146160"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..1084
                     /locus_tag="VE646_04880"
     CDS             212..1084
                     /locus_tag="VE646_04880"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HZD79357.1"
                     /translation="MKDLVGPADFTLANCGTIIIRKVTDPSGDTTTSFGYTTNVTTTP
                     STTTSPFSLKDGEHNKIENVNPGSSLNVTEDDPGPGYALTNIDCSASTVSSANYSTDT
                     STRAVTFSIAAGETLDCTFTNTLQRGAIEITKTTIKDDSGLAGATFSITGPNSYSNSV
                     SSGSDGTVCLDGLTFGDYTVTETAAPSGYAIDDDAGHTVTVDNNATCSDDPFGGETIS
                     FSDTPLSEVGVTFTSLAGSGVTNASIVCEDSTPSVIDAVSENGDPDPAYDDTGETFTN
                     LAPGTYTCTVVVDP"
     gene            1301..2131
                     /locus_tag="VE646_04885"
     CDS             1301..2131
                     /locus_tag="VE646_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006911143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZD79358.1"
                     /translation="MRVAVVGHVEWVDFARVDHVPSAGEIVHASDWWAEPAGGGAVAA
                     VQLAKLAGDCTFFTALGRDEVGRRAAEELRRLGLRVKVAWRHAPTRRALTFVDPRGER
                     TITTLGPRLNPHAEDQLPWDQLASTDAVYFTAGDEGALRIARRGRVLVPTSRVMGLLG
                     RVDIPLDAVVGSARDPSERFDPSALSRPPGLVVLTDGARGGTFLTPDGSDRAFAAAPL
                     PGPLVDTYGGGDSFAAGLTYGLGAGMPPERALRLAARCGAWCVSGRGPYAGQLTAADL
                     "
     gene            complement(2244..3377)
                     /locus_tag="VE646_04890"
     CDS             complement(2244..3377)
                     /locus_tag="VE646_04890"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TQO small subunit DoxD"
                     /protein_id="HZD79359.1"
                     /translation="MNQRKARTQRNRRLPPPPVRGRHRGGSPLVQGWRQQPMSMRLLR
                     AFLGVTFVYAGVQKMADPNFLHPGTPDFIGSQLAAFAHGSPIGGVLRALAQVPVPVGL
                     AIAFAEIAIGLGTLVGIAPVLMGVGGFLVNLALTLSATWHVHPYFLGSDSIYAVAWLA
                     YLLGLVELGRRARSRAAPTVDRIHTSDEIGRRELIRGGVLAGATLLAASAGAAVAGKP
                     AAALSAGLKQRRPGRGQDGVPSQDPTGNGSSGSSSQGPGVSSSQGSSQGSGGPSPSGT
                     TIATLSSLPVGGAVGFNDPGVGPAALIRLGQDRVVAYSRVCTHAGCLVGYDASQQILY
                     CPCHGAEYDPAHHAEVIAGPAPSPLPPIKVEIDPATGTVILPS"
     gene            3481..4137
                     /locus_tag="VE646_04895"
     CDS             3481..4137
                     /locus_tag="VE646_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD79360.1"
                     /translation="MRLLLIEDDRRLADLLATRLRKEGYEAATCASGLEGLDMAASGE
                     YDLAVVDVMLPGLDGISLTAELRSRGIAIPVLMLTARDTVEDRVTGLRSGADDYLVKP
                     FAFAELLARIEALVRRMDGGDRLAHDGVVLDPLARRVWAADRAVELTAKEFDLLECLL
                     RNRGRVMTRAELLEQVWDFALETQGKVVDLYVHYLRKKLGDSGSVIQTVRGVGYAIGR
                     "
     gene            4162..5316
                     /locus_tag="VE646_04900"
     CDS             4162..5316
                     /locus_tag="VE646_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZD79361.1"
                     /translation="MAASHATVLAVILIALGGLGLLLLSRELDRAATGELRAAAQQQA
                     NRIREMGHPAPPPDSDVPSASALQLGVFLPDGTPIGEPAEIPSWLTPGPPVRDLTISS
                     EPVRLVTLPAGPSGAPLALVVAARSLIPEAHLLHEVRLLLLFGGGAAVALSLAAGWWL
                     AGRALRPVQAAYEVQAGFAADASHELRTPLTFIRSGVEVLAERDPELGAQVLSEVDYL
                     TELTRQLLELARAERGTLSLERRPIDVGRICRSAAQRSRMASGTTLSLEGEEVAATGD
                     PIAVEAALDAMLENVGVHGGGSATVRWGPVDGRVLISVEDRGPGLAPELASRAFERFT
                     RADPSRTRDTGGAGLGLALARALVEAQGGRMWLEPTPGGGLTAKIALPRG"
     gene            5363..7195
                     /locus_tag="VE646_04905"
     CDS             5363..7195
                     /locus_tag="VE646_04905"
                     /inference="COORDINATES: protein motif:HMM:NF015410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HZD79362.1"
                     /translation="MDVSAFLARTPPFDTLDGSTLARLVRSVQIEHFPPGAPMVPPGA
                     EAPRFLYLIRKGAVEVIDGDTPLDLMTEGEAFGAGSPLDQLGETVRLRAHEDTLCYLI
                     PSEIAEEVLHTRRGMAFVMGTVRRTIARLREERVTDRPVEMYRPVGSLIRRPPVTCGA
                     GTSVAEAARLMARERISCVLVAGGEGWGILTDRDLRSRVVAEGRDPERITVSEVMTPA
                     ATTVEPSELAGEVLLRMLEHGFHHCPVRAGDGRLLGVVTDTDLMGLGRETPFALKSAI
                     ERAPDAEAAVREVRRLPQVISALVEAGSDPVETGHVVAFAIDALTRRLLDLGIDRLGE
                     PPVPWAWLALGGAARQEQALRTDQDHALVVGAGSEEPEEPVDAYFAELATLVTDGLET
                     ADIPRCEGHAMATNRVLRTSIGGWERRLHGWMEDPSLMGSVFLSIAFDRRQVAGPLDA
                     EPILGAMMHQASAYPLFLRHLARRGLDLRPPTGFFRDVVVGSQSERAGRLDLKQGGIT
                     IVNNLARYWAIRAGVPARRTVERLRATGGRGGGHEDLVEAFRFLWQVRLRHQVEQVGR
                     GTDPDDLVDPRELGPVVRQGLKEAFRTIGRAQRVLALELGVPRR"
     gene            complement(7203..8003)
                     /gene="fabI"
                     /locus_tag="VE646_04910"
     CDS             complement(7203..8003)
                     /gene="fabI"
                     /locus_tag="VE646_04910"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HZD79363.1"
                     /translation="MLLQGKKLLITGVLTRESIAFATARAVQREGAEIVLSSFGRALS
                     ITEKLAKRLDPQPDVLELDANDEDHIQAVREELARRWGSLDGFLHAIAFAPQDALGGN
                     FLTTPWESVGAAFRTSAFSLKAIAAGLLPLMRQRGGGIVSLDFDARVAWPVYDWMGVA
                     KAGLESVTRYLARDLGPYGVRVNAVSAGPLRTVAGKGIPGFEELAERWHERAPLGWDT
                     TDPSPVADTVAFLLSDAARGISGELVHVDGGYHALGSNPVAPPTDTHG"
     gene            8111..9124
                     /gene="add"
                     /locus_tag="VE646_04915"
     CDS             8111..9124
                     /gene="add"
                     /locus_tag="VE646_04915"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HZD79364.1"
                     /translation="MDLRTVAKVDLHRHLEGALRLQTIIDLYREAGEPLAAATPAELA
                     PAAQVLAPMGSLAEVLTFFRIAQGALRTYQAVERVAFEAVEDLAADHVRLAELRYSPD
                     FLCRSHGLDWDGALDAIVAGVERARAVHDVTVGLVAIVSRSYGMESAARTVAFALRHR
                     DRFVGFDLADDEVNHPPSDYVQVLAPLREARLPLTAHYGEAAGPQYPRDAVQVLGARR
                     LGHGVSVAADPAVTALVIDRGVTLEMCPTSNHRTNAVASLEAHPALRLLHQGARVTLN
                     TDDPGLFGIDLTHELEVAADVLGFGPGDLRLATEHALQASFLDPRVKAETRRRHFGWL
                     DAT"
     gene            9275..9886
                     /locus_tag="VE646_04920"
     CDS             9275..9886
                     /locus_tag="VE646_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HZD79365.1"
                     /translation="MPKVDLKRSMRRLYFPPAGQPVLVDVPELSFLRIDGRGDPEAAP
                     YTQAMEALFAVSFGLKFMIKRLDPEDDYTVMPLESLWWSRDQSTFEIEDRSTWRWTAM
                     VVQPSNVTGSLVERAVADAAAKRRSPAFRKIRLERFREGLAAQVMHVGPYSEELATIE
                     KLHAFIAEHDYPVRGKHHEIYLSDPRRCAPDRIKTVIRQPVGL"
     gene            9987..11633
                     /locus_tag="VE646_04925"
     CDS             9987..11633
                     /locus_tag="VE646_04925"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZD79366.1"
                     /translation="MTTADAVRVFVDRYWEQLLELDPILGTMVGDERYDDRLPDPSEE
                     GRARSASVHRAALAELASFDRDIEDDALRTSLDMAEAISLREVASIDHRLDRLWAVSH
                     FWGPAQLLGELGAMQRADTPERLERYLARLSATPAFYHAVREVTRDGAAAGVTAPRVV
                     VERTLGQLERFLTTPVEESPALGPVPEQDTAGRERVAEVLRTSAMPALEAYRTALREY
                     LPDVTETIGLSALPGGEAMYAAEILAWTSLPLEAQKVHDLGVEDLERIEEERRLAADR
                     LGYGSPAEAEADLLQRHPFASKEELKDLAEGQVRRGWEAAPSFFGRIPRDNCEVRSVE
                     EFREADMAFAFYQPPSEDGSRPGIYYVNGYDLPSRPRHHLATTTYHEANPGHHFQISI
                     EQEMGDRPALRRFGGLLSGSAFAEGWGLYSERLADEMGLFLDEAERLGMLDAQAHRAA
                     RLIVDTGMHALGWTRERSIATLASAGVPHTDAVIETDRYITLPGQALSYKIGQFEIER
                     QREEAAKREGAAFTLPSFHDRLLALGSLPLPALQRELRSR"
     gene            11772..12956
                     /locus_tag="VE646_04930"
     CDS             11772..12956
                     /locus_tag="VE646_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HZD79367.1"
                     /translation="METGRILVGVPRETFPGESRVALVPAALPVLAKAGMDVIVEHGA
                     GEAAGFRDASYEKNGGRLGARVDVFEAEIVVQVRTCGANPDAGRDDIERYHPDQIIAG
                     MADPLVCPIGIQQAAERKVIAFALDFMPRITRAQVMDVLSSQATAVGYKAVLLAAERL
                     PKLFPMLTTAAGTVPPAQVFVIGAGVAGLQAIATARRLGAVVQGYDVRPAAQEDIESL
                     GGRAVLLPLQPGDAEDKSGYAKELGEAFYRRQQEFMAQVVAGSDVVISTALIPGKKAP
                     VLITEEAVARMQPGSVIVDCAAERGGNCALTRPNRRVVTDNRVTILGPTNLASSVPTV
                     ASQMYSKNVTAFLTHVFKDGSLDIDFEDEIVKGTLVTRAGDIVHTRVLESLAEVGHDG
                     SA"
     gene            12940..13266
                     /locus_tag="VE646_04935"
     CDS             12940..13266
                     /locus_tag="VE646_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HZD79368.1"
                     /translation="MTGQLEVALFIFVLASFLGYAVITRVPGLLHTPLMSLTNALDAI
                     AIVGSLILAGAGETTMATVLGSVAVFASFTNIVSGFLLTDRMLRMFKRREAPQRSRAA
                     PKGAQS"
     gene            13275..13721
                     /locus_tag="VE646_04940"
                     /pseudo
     CDS             13275..13721
                     /locus_tag="VE646_04940"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
     gene            complement(13754..13931)
                     /locus_tag="VE646_04945"
                     /pseudo
     CDS             complement(13754..13931)
                     /locus_tag="VE646_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
BASE COUNT         1960 a         4910 c         5058 g         2003 t
ORIGIN      
        1 cgacgcggga tacgcggagg cagaggtcaa cacgggagga gcggtcaccg actcgatcgc
       61 gccaagcccg ccggaatcac tcgggacgaa cgagttcggc gaggcgggga tcgatctcac
      121 cggcgccggt gtgttcagtg ccggaacgtg cgagagcttc ggaacggcgt tcggcgtcag
      181 ccgcagctcc ggcaactctg ggaccgcgca gatgaaggac ctcgtggggc cggcagactt
      241 cacgctcgcc aactgcggca cgatcatcat ccgcaaggtg acggacccgt ccggagacac
      301 gacgacttcc ttcggctaca cgaccaacgt gacgacgacg ccgtcaacca cgacctcacc
      361 cttctcgctc aaggatggcg agcacaacaa gatcgagaac gtcaacccgg gcagcagcct
      421 gaacgtcacc gaagacgacc cgggccccgg ctacgcgctg acgaacatcg attgctcggc
      481 cagcactgtc tcgtcggcca actactcgac cgatacgtca acgagggcgg tgacgttctc
      541 gatcgccgca ggtgagacgc tcgactgcac cttcacgaac acactgcagc gcggcgcgat
      601 cgagatcacc aagaccacga tcaaggacga cagcggtctg gcgggcgcca cgttctcgat
      661 caccgggccg aactcgtact cgaactcggt gtcgagcggc tccgatggca ccgtgtgcct
      721 ggacggcctg acgttcggcg actataccgt cacggagacg gcggcaccga gcgggtacgc
      781 catcgatgac gacgccggtc acaccgtcac cgtcgacaac aacgcgacgt gcagcgatga
      841 tccgtttggt ggggagacga tctcgttcag cgacacgccg ctctcggagg tcggtgtgac
      901 gttcacgtcg ctggcaggat cgggagtgac caacgccagt atcgtgtgcg aggactccac
      961 gccctctgtg atcgacgcag tgtcggagaa cggcgaccca gatccggcgt atgacgacac
     1021 gggcgagacc ttcaccaacc tcgctccggg cacgtacacc tgcacggtgg tggtcgaccc
     1081 gtagcgagcg agagctaaca gaaagatcgc gagaggggcg gccccacccg ggtcgccctt
     1141 ctcggttccc ggctcctggg gaagctgcag gctcgccagc ccccttccgg tctgaccagt
     1201 gcgttcccgg caggtgatgc ggacctgagg gatcgtggga ccccacgatg cggtggggtc
     1261 agatccgatg aggcaagctc agccaccgta cgctggcccc gtgcgggtgg cggtggtggg
     1321 ccacgtggag tgggtggact tcgcccgcgt cgaccatgtc ccaagcgcgg gggagatcgt
     1381 gcacgcctcc gactggtggg cggagcctgc cggcggcggc gcggtggcgg ccgtgcagct
     1441 cgcgaagctc gcgggggact gcaccttctt caccgccctc ggcagggacg aggtgggacg
     1501 gcgggcggcc gaggagctgc gtcgcctggg cctccgggtg aaggtggcct ggcgccacgc
     1561 cccgacgcgt cgcgcgctga ccttcgtcga tcccaggggc gagcggacga tcacgacgct
     1621 cggccctcgc ctgaacccac acgccgagga ccagctcccc tgggaccagc tcgcgagcac
     1681 cgacgcggtg tacttcaccg ccggcgacga gggggccctg cggatcgctc gacgaggtcg
     1741 ggtcctggtc ccaacctcgc gggtcatggg gctcctgggg cgggtcgaca tccccctcga
     1801 cgcggtggtg gggagcgccc gcgacccctc ggagcggttc gacccgtccg ccctgtcgcg
     1861 gcctcccggc ctggtggtgc tcaccgacgg ggcacgcggc gggaccttcc tcacccccga
     1921 cgggagcgac cgagcgttcg cggccgcccc cctgcccggc ccgctcgtgg acacctacgg
     1981 gggcggcgac agcttcgcgg ccggtctcac ctacggcctg ggggccggga tgccgcccga
     2041 gcgagcgctc cggctggccg ccaggtgcgg cgcctggtgc gtgtccggcc gggggccgta
     2101 cgcgggtcag ctgacagccg ccgacctctg atcgatcacg gtccccgacg cggacggggc
     2161 ctcgacctgg cggagcgggt ccacgacgcg gttgttccgt cgagccggca tgcccacccg
     2221 acgcgggggg tcgtgccggg cgctcaggac ggcaggatca ccgtgcctgt agccgggtcg
     2281 atctccacct tgatcggcgg gagcgggctg ggcgcgggac cggcgatcac ctcggcgtgg
     2341 tgcgcggggt cgtactcggc cccgtggcag gggcagtaca ggatctgctg ggaggcgtcg
     2401 tagccgacca ggcatcccgc gtgcgtacac acgcggctgt aggcgaccac gcgatcctgt
     2461 cccaggcgga tcagcgcggc cggtcccaca ccgggatcgt tgaacccgac ggcgcccccc
     2521 accgggaggc tgctgagggt ggcgatcgtg gtcccggacg gcgagggacc gcccgagccc
     2581 tgggacgagc cctgggacga agaaacgccg ggaccctggg acgacgagcc gctcgagccg
     2641 ttccccgtcg ggtcctggga ggggacgccg tcctgtcccc gcccgggccg gcgctgcttc
     2701 aagccggcgc tcagcgccgc ggcgggcttc ccggccaccg ccgcgccggc gctggcggcg
     2761 agcagcgtgg cgccggccag cacgcctcct cggatcagct ctctgcggcc gatctcgtcg
     2821 gacgtatgga tccgatcgac tgtgggagca gcccggctcc gggcccgccg gcccagctcg
     2881 accaggccca ggaggtaggc gagccacgcc accgcgtaga tcgagtccga tcccaggaag
     2941 tagggatgca catgccaggt ggccgacagc gtcagggcca ggttgacgag gaagccccca
     3001 acgcccatca gcaccggcgc gatgccgacc agcgtgccga gcccgatcgc gatctccgcg
     3061 aacgcgatgg ccagccccac cggcaccgga acctgggcca gcgcccggag cacgccaccg
     3121 atcggactgc catgggcgaa ggcggcgagc tggctgccga tgaagtcggg ggtgcccggg
     3181 tgcaggaagt tgggatcggc catcttctgc accccggcgt acacgaacgt gacgccgagg
     3241 aacgcgcgga gcagtcgcat cgacatcggc tgctgccgcc acccctggac caggggcgag
     3301 cctccccggt gccggccccg gaccgggggc ggagggagcc ggcggttgcg ctgggtgcga
     3361 gccttcctct ggttcacggt gaccgagtat ccggatcgcg gattggacga cgtttggccg
     3421 aaggttggga tcgggttagg aacgagggtc atccggcctc gctcgcgaga ccatagggac
     3481 gtgcgtctgc tcttgatcga ggacgaccgc cggctggccg acctgctcgc gacccggctc
     3541 cggaaggagg gctacgaggc cgccacctgc gcgtcgggtt tggagggtct ggatatggcc
     3601 gcctcgggcg agtacgacct ggccgtggtg gacgtgatgc tccccgggct ggacggcatc
     3661 tccctcaccg ccgagctccg gtcgcggggc atcgcgatcc cggtgctgat gctcacggca
     3721 cgcgacaccg tggaggatcg ggtgacgggc ctgcgctcgg gcgcggacga ctacctggtc
     3781 aagcccttcg ccttcgccga gctcctggcg cgcatcgagg ccctggtccg gcgcatggac
     3841 ggcggggacc ggctggctca cgacggcgtg gtcctcgacc cgctggcacg ccgcgtgtgg
     3901 gcggccgacc gagcggtgga gctgaccgct aaggagttcg acctgctcga gtgcctgctg
     3961 cgcaatcgag gacgggtgat gacgcgcgcc gagctcctgg agcaggtctg ggacttcgcg
     4021 ttggagacgc agggcaaggt ggtggacctg tacgtccact acctcaggaa gaagctggga
     4081 gactccggga gcgtgatcca gaccgtgcgc ggcgtcgggt acgcgatcgg ccggtagatg
     4141 ttccgctccc ttcgcttccg tatggccgca tcccacgcca cggtgctggc ggtcatcctc
     4201 atcgctctgg gtgggttggg tctgttgctc ctgtcccgcg agctcgaccg ggcggccacc
     4261 ggcgaactgc gggccgcggc gcagcagcag gcgaaccgga tccgagagat ggggcacccg
     4321 gcgcccccac ccgactcgga cgtgccctcc gcgtcggccc tacagctcgg cgtcttcctg
     4381 cccgacggca ccccgatcgg cgagccggcc gagatcccca gctggctgac cccggggccg
     4441 cccgtccgcg acctcacgat ctcttccgag ccggttcgtc tggtgaccct gcccgcgggc
     4501 ccctccgggg cccccctggc cctggtggtg gcggcccgat cgctgatccc cgaggcccac
     4561 ctgctccacg aggtacggct gctcttgctc ttcgggggcg gggcggcggt ggccctcagc
     4621 ctggccgccg gctggtggct ggcggggcgg gccctccgtc cggtccaggc cgcctacgag
     4681 gtgcaggcgg ggttcgccgc cgatgcctcc cacgagctgc ggacgccgct gacgttcatc
     4741 cgctcgggag tggaggtgct ggccgagcga gacccggagc tgggcgccca ggtgctctcg
     4801 gaggtcgact acctcaccga actcacccgg cagctcctgg agctggccag ggccgagcga
     4861 ggcaccctgt cgctggagcg gcggcccatc gacgtggggc ggatctgccg ctccgccgcg
     4921 cagcggagcc ggatggcgtc ggggaccacc ctgtcgctgg agggagagga ggtcgccgcg
     4981 accggggatc ccatcgccgt cgaggcggcg ctggacgcga tgctcgagaa cgtcggcgtc
     5041 cacgggggcg gctcggccac ggtgcggtgg ggcccggtcg acggacgggt gctgatctcg
     5101 gtcgaggacc gcgggccagg cctggcgccc gagctggcct cgcgcgcgtt cgagcggttc
     5161 acgcgggccg atcccagccg gacccgagac acggggggcg ccggtctcgg gctggcgctg
     5221 gcgcgcgccc tggtcgaggc gcagggcggc aggatgtggc tcgagcccac gcccgggggt
     5281 ggcctgaccg ccaagatcgc cctgccccgc ggctgagccg cgcctggccc gggtgccgcg
     5341 gcctgccgta ggctccggac cggtggacgt ctccgcgttc ctggcccgga ccccgccgtt
     5401 cgacacgctg gacggatcca cgctcgcacg cctggtgcgc tcggtccaga tcgagcactt
     5461 ccctcccggc gcgcccatgg tgcctccggg cgccgaggcg ccgaggttcc tgtacctgat
     5521 ccgcaagggg gcggtcgagg tgatcgacgg cgacacaccc ctggacctca tgaccgaagg
     5581 tgaggcgttc ggcgccggct ccccgctgga ccagctgggg gagaccgtcc ggctccgcgc
     5641 gcacgaggac acgctctgct acctgatccc gagcgagatc gctgaggagg tgctccacac
     5701 gcggcgaggg atggcgttcg tgatggggac ggtgcggcgc acgatcgccc ggttgcggga
     5761 ggagcgcgtg accgatcgtc cggtcgagat gtaccggccg gtcggctcgc tgatccggcg
     5821 cccgccggtg acgtgcgggg ccggcaccag cgttgccgag gcggctcggc tcatggcgcg
     5881 cgagcggatc tcctgcgtgc tggtcgccgg tggggagggg tggggcatcc tgaccgatcg
     5941 cgacctgcgg tcccgggtgg tggccgaggg gcgcgatccc gagcggatca cggtgtctga
     6001 ggtcatgacc ccggccgcca ccacggtcga gccctcggag ctggccggcg aggtcctcct
     6061 gcggatgctc gagcacggct tccaccattg cccggtgcgc gccggggacg gccgtctgtt
     6121 gggcgtggtg accgacacgg acctaatggg cctgggccgc gagaccccgt tcgcgctgaa
     6181 gagcgcgatc gaacgcgccc ccgatgccga ggccgccgtt cgggaggttc gacggctccc
     6241 ccaggtgatc tcggccctcg tggaggccgg cagcgacccg gtggagaccg gtcacgtggt
     6301 ggcgttcgcg atcgacgcgc tgacccgtcg cttgctggac ctcgggatcg atcggctggg
     6361 cgagccgccg gtcccctggg cctggctggc cctgggggga gcggcccgac aggagcaggc
     6421 cctccgcacg gaccaggacc acgccctggt ggtaggcgcg ggatccgagg agccggagga
     6481 gccggtggac gcctacttcg cggagctggc caccctggtg acggacgggc tcgagacggc
     6541 cgacatcccc cgctgcgagg gccacgcgat ggccacgaac cgagtgctgc gcacctccat
     6601 cgggggctgg gaacggcggc tccacggatg gatggaggac cccagcctga tgggcagcgt
     6661 cttcctgtcg atcgcgttcg accgcaggca ggtggccggg ccgctggatg ccgaacccat
     6721 cctcggcgcg atgatgcatc aggcttcggc gtaccccctg ttcctccggc atctggcgcg
     6781 tcggggcctc gacctccgcc cgcccaccgg gttcttccgc gacgtcgtgg tcggatcgca
     6841 gagcgagcgg gcggggaggc tcgacctcaa gcagggcggc atcacgatcg tgaacaacct
     6901 cgcccgctac tgggcgatca gggccggcgt tcccgcgcgg cgcacggtgg agcgcctgcg
     6961 ggccacgggc ggccgcggcg gagggcacga ggacctggtg gaggcgttcc ggttcctgtg
     7021 gcaggtgcgg ctgcggcacc aggtcgaaca ggtcgggcgt ggcaccgatc cagacgacct
     7081 ggtggacccg cgcgagctcg gcccggtggt gcgacagggc ctgaaggagg ccttccggac
     7141 gatcgggcgg gcccagcggg tgctggccct ggaactcggg gtgccccggc gctgagtcga
     7201 ccctagccgt gggtgtcggt cggaggagcg accgggttcg atccgagggc gtggtagccc
     7261 ccgtccacgt ggaccagctc gccgctgatg cctcgtgcgg cgtccgagag caggaacgcg
     7321 accgtgtcgg ccaccgggct cggatcggtg gtgtcccatc ccagcggcgc ccgctcgtgc
     7381 caccgctcgg ccagctcctc gaagccgggg atccccttgc cggcgaccgt gcggagcggg
     7441 ccggctgaca cggcgttcac gcggaccccg tacggaccca gatcgcgcgc caggtaccgg
     7501 gtgaccgact cgagccccgc cttggctacc cccatccagt cgtacaccgg ccaggccacc
     7561 cgcgcgtcga aatccaagga cacgatgccg cccccgcgct gccgcatcag cggcaggagg
     7621 ccggcggcga tcgccttcag ggagaaggcg ctggtccgga aggccgcgcc cacgctctcc
     7681 cagggcgtgg tgaggaagtt gccgcccaat gcgtcctggg gagcgaaggc gatcgcgtgc
     7741 aggaacccgt ccagggatcc ccaccgccgg gccagctcct cgcggacggc ctggatgtgg
     7801 tcctcatcgt tcgcgtcgag ctccagcaca tccggctgcg ggtccaagcg cttggcgagc
     7861 ttctcggtga tgctgagggc ccggccgaac gagctgagga cgatctcggc gccctcgcgc
     7921 tggacggctc gagcggtcgc gaacgcgatg ctctcccggg tcagcacccc ggtgatcaac
     7981 agcttcttgc cctgcagcag catccgcgcc ccctcgacga acggcctccg atgctaccgg
     8041 cgtgcggggc cccccgcatc ctcgcatcat cggccgaggc cccccacacg tcgtttaccc
     8101 ttcggaggtc atggaccttc ggaccgtggc caaggtcgat ctgcaccggc acctggaggg
     8161 agcgctccgc ctccagacca tcatcgacct gtaccgcgaa gccggggagc cgctcgcggc
     8221 cgccaccccc gccgagctgg cgccggccgc acaggtcctg gccccgatgg gcagcctggc
     8281 cgaggtcctg acgttcttcc gcatcgctca gggggccttg cgcacctacc aggccgtgga
     8341 gcgggtcgcc ttcgaggcgg tggaggacct ggccgccgac cacgtccggc tcgccgagct
     8401 ccgctactcc cccgacttcc tgtgccgcag ccacggactg gactgggatg gggcgctgga
     8461 cgccatcgtg gccggcgtgg agcgggcccg cgcggtacac gatgtcacgg tgggcctggt
     8521 ggcgatcgtc agtcgctcct acggcatgga gtcggcggcg cggaccgtgg ccttcgcgct
     8581 gcgtcaccgg gaccgttttg tgggcttcga cctcgcggac gacgaggtga accatccgcc
     8641 ctcggactac gtgcaggtgc tggccccgct ccgggaagcg cggcttcccc tcaccgccca
     8701 ctacggggag gcggcggggc cccagtaccc gcgggatgcg gtgcaggtgc tcggcgctcg
     8761 gcgcctgggt cacggggtca gcgtggcggc cgaccccgcc gtgacggccc tggtgatcga
     8821 ccggggggtc accctggaga tgtgccccac ctccaaccac cggaccaacg ccgtcgcctc
     8881 gctggaggcc caccctgcgc tccgtctcct gcaccagggt gctcgcgtca ccctgaacac
     8941 ggacgatccc ggcctgttcg gcatcgacct cacccacgag ctggaggtgg ccgccgacgt
     9001 gttggggttc ggtccgggcg acctgcggct cgccaccgag cacgccctgc aggcgagctt
     9061 cctggatccc cgggtcaagg ccgagacgcg ccgtcggcat ttcggctggc tcgacgctac
     9121 ctagggccgg ggcccgagga ccggttccag ccggccgggc gcccctgggt cgccgacccg
     9181 agccgacggg cgggcggcat gggcagggca ggtcgttcgc ccctaatggc ccgctcgggc
     9241 ccctggctac ggtggcgccc gagaggaggg ccggttgccc aaggtcgacc tcaagcgatc
     9301 catgcgtcgc ctgtacttcc ctccggccgg gcagccggtc ctggtggacg tccccgagct
     9361 gtccttcctc cggatcgacg gtcggggcga tccggaagcg gcaccgtaca cccaggcgat
     9421 ggaggcgctg ttcgccgtct ccttcgggct gaagttcatg atcaaacgcc tcgatcccga
     9481 ggacgactac acggtgatgc cgctggagtc gctgtggtgg agccgggacc agagcacgtt
     9541 cgagatcgag gaccggtcga cctggcggtg gacggcgatg gtcgtccagc cctcgaacgt
     9601 caccgggtcg ctcgtggagc gagccgtggc cgatgccgcc gcgaagcgtc gttcgcccgc
     9661 gttccgcaag atccggctgg aacggttccg ggagggactg gcggctcagg tgatgcacgt
     9721 ggggccctac tcggaggagc tcgcgaccat cgagaagctc cacgccttca tcgccgagca
     9781 cgactacccg gtgcggggga agcatcacga gatctacctc tccgatcccc ggcggtgcgc
     9841 gccggaccgg atcaagaccg tgatccggca gcccgtcggt ctgtagcggc cggcgtcctc
     9901 gcgctccagc gggaccagcg tgccccactg agccggtcgg gtcggactag gatggcccgc
     9961 gccacggtcg gagaggggaa cgagggatga ccaccgccga cgcggtccgc gtgttcgtgg
    10021 accgctactg ggaacagctg ctggagctcg atcccatcct gggcacgatg gtcggcgacg
    10081 agcgctacga cgatcgcctc cccgacccga gcgaggaggg ccgggcccgg tcggcttcgg
    10141 tgcaccgagc cgcgctggcc gagctcgcga gcttcgaccg ggacatcgag gacgacgcgc
    10201 tccggacctc gctggacatg gccgaggcca tctccctgcg cgaggtcgcc tccatcgacc
    10261 accgcctcga tcgcctctgg gccgtgagcc acttctgggg tccggcccag ctcctcggcg
    10321 agctcggcgc catgcagcgg gccgacacgc ccgagcggct ggagcgctac ctggcgcgcc
    10381 tgtccgcgac gccggccttc taccacgcgg tgcgcgaggt cacccgggac ggggccgccg
    10441 cgggcgtcac cgcccctcgg gtggtggtcg agcggacgct gggccagctc gagcggttcc
    10501 tgaccacgcc ggtcgaggag tcacccgcgc tgggccccgt gccggagcag gacaccgccg
    10561 gacgcgagcg cgtggccgag gtcctgcgga catcggccat gccggccctg gaggcctacc
    10621 ggaccgccct gcgcgagtac ctgcctgatg tcaccgagac gatcggcctg tcggcgctcc
    10681 cggggggcga ggcgatgtac gcggccgaga tcctggcctg gacctcgctg ccgctggagg
    10741 cccagaaggt gcacgacttg ggcgtggagg acctggaacg gatcgaggag gagcggcgtc
    10801 tagcggccga ccggctgggc tacggttcgc ctgccgaggc cgaggccgac ctcctgcagc
    10861 gtcatccgtt cgcctcgaag gaggagttga aggacttggc cgagggccag gtccgtcggg
    10921 ggtgggaggc ggcgccgtcc ttcttcggcc ggatcccccg cgacaactgc gaggtccgat
    10981 cggtggagga gttccgggag gcggacatgg cgttcgcctt ctaccagcct ccctccgagg
    11041 acggctcccg gccggggatc tactacgtga acggctacga cctccccagc cggccccggc
    11101 accacctggc caccaccacc taccacgagg ccaaccccgg tcatcacttc cagatctcga
    11161 tcgagcagga gatgggcgac cggccggcct tgcgtcggtt tggcgggctg ctgtccggca
    11221 gcgccttcgc cgaagggtgg gggctgtaca gcgagcggct ggccgatgag atgggcctgt
    11281 tcctggacga ggccgagcgg ctcggcatgc tcgacgccca ggcccaccgg gccgcgcggc
    11341 tgatcgtgga caccgggatg catgcgctgg gatggacacg ggagcggtcc atcgccacgc
    11401 tggcgtcggc gggcgtgccc cacacggatg ccgtgatcga gaccgatcgc tacatcacgc
    11461 tgcccgggca ggccctctcg tacaagatcg gccagttcga gatcgaacgg cagcgggagg
    11521 aggcggccaa gcgggagggt gccgcgttca cgctcccctc cttccacgac cgcctgctcg
    11581 ccctgggctc gctgcccctg ccggcgctcc agcgcgaact gcggagccgc tgagccggcg
    11641 ctcacccgga ccgagccctc caccggccct cgcccctccg gccgaacggc cccgtggcgg
    11701 cgggcctccc gccactctcc gacctcggtc gcgcctggga acgtgatggc gatagcgagg
    11761 acggtgagct catggagaca ggacgcatcc tcgtcggtgt ccctcgggag accttcccgg
    11821 gggagtcacg ggtggccctc gtgccggcgg ccctcccggt cctcgccaag gccggcatgg
    11881 acgtgatcgt cgagcacggc gcgggcgagg cggcggggtt ccgggacgcc agctacgaga
    11941 agaacggggg acggctcggg gcacgcgtcg acgtgttcga ggccgagatc gtggtgcagg
    12001 tccggacctg cggggccaac ccggacgcgg gccgcgacga catcgagcgc taccaccccg
    12061 atcagatcat cgccggcatg gccgaccccc tggtctgtcc gatcggcatc cagcaggccg
    12121 ccgagcgcaa ggtgatcgcg ttcgccctgg acttcatgcc ccggatcact cgggctcagg
    12181 tcatggacgt gctctcctcg caggccacgg cggtgggcta caaggccgtg ctgctcgcgg
    12241 ccgagcgcct gccgaagctc ttccccatgc tgacgacggc cgccggcacc gtcccccccg
    12301 cccaggtctt cgtgatcggg gccggggtgg cagggctcca ggccatcgcc accgcccggc
    12361 ggctcggcgc ggtggtccag gggtacgacg tccggccggc ggcgcaggag gacatcgaga
    12421 gcctcggcgg ccgcgccgtg ctgttgcccc tgcagcccgg ggatgccgag gacaagagcg
    12481 ggtacgccaa ggagctgggc gaggccttct accgccgcca gcaggagttc atggcccagg
    12541 tcgtggcggg ctcggacgtg gtcatcagca ccgcgttgat ccccggcaag aaggccccgg
    12601 tgctgatcac cgaggaggcc gtggcccgga tgcagccggg ctcggtcatc gtggactgtg
    12661 cggcggagcg cgggggcaac tgcgccctca cccgcccgaa ccggcgggtc gtgacggaca
    12721 accgggtcac gatcctgggt cccaccaacc tggcctcgtc ggtccctacc gtggccagcc
    12781 agatgtactc gaagaacgtc accgcgttcc tcacgcacgt gttcaaggac gggagcctcg
    12841 atatcgactt cgaggacgag atcgtgaagg gaacgctcgt caccagagcc ggcgacatcg
    12901 tccacacccg ggtgctggag tcgctcgcgg aggtgggtca tgacgggtca gcttgaggtc
    12961 gcgctcttca tcttcgtgct ggcctcgttc ctggggtacg ccgtcattac gcgtgtgccc
    13021 ggattgctgc acacgccgct gatgtcgctc acgaacgccc tggacgcaat cgcgatcgtc
    13081 gggtcgctga tcctggctgg tgccggcgag accacgatgg ccacggtgct cggctccgtg
    13141 gcggtcttcg catccttcac gaacatcgtc tccggcttcc tgctcaccga ccgcatgctg
    13201 cgcatgttca agcgccgtga agccccccag cgctcccggg ctgcccccaa gggagcccag
    13261 tcgtgaagga cgcgatgaac acgaccgtca tcccgctcgc ctacctggtg gcggcgtcgt
    13321 gcttcgtgct ggcgctgaag tggctctccc accccaccac cgctcagcgc ggggtccggg
    13381 tcggtgaggt ggggatgttc ctggcgatcg tggccacgct gttcaagttc cagatcgtcg
    13441 agtaccagtg gatcgcgatc gcgctggtcg cgggcgtgat cgtgggcatc ccgctcggcc
    13501 tggtcccgat gacggcggtg ccgcagcgga cggcgctctc ccatgccttc ggcgccctgg
    13561 ccgccggcgt ggtgggcacc gccgagtact acctgcgacg gcccgacctg agcaccagca
    13621 cgatgatcgc cctcggtctg gaggtggtgc tggggttcct gacgttcaca ggctcgttga
    13681 tcgccgcagg caagctccag ggggtcctgc gggaccggcc ctggatctac aagggccaga
    13741 acatcgtgaa cctggccgtg ctggcctcgg ccagcaggac gttcatgtgc ccgggcatcc
    13801 gctcggccac cgggtggatc gcgaaccgca cgtccacccc gcggtgcagc agcgcgtcgg
    13861 agagctcgcg caccttgtgc tgggcctgag ccgcggccat cccgtacccg gggacgatca
    13921 ccacccggcg g
//
LOCUS       DASQQQ010000117         7980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_364637, whole genome shotgun sequence.
ACCESSION   DASQQQ010000117 DASQQQ010000000
VERSION     DASQQQ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364637"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1836
                     /locus_tag="VE646_04950"
     CDS             <1..1836
                     /locus_tag="VE646_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HZD79369.1"
                     /translation="GGEPDLDVLLPVAVALPLFGAAALAALTKLLPRRPADLLALGFA
                     CATTAICALLTIVAASGPVIHWFGGWTPLGRQAVGVAFLADGFAAGFAAFIGLLVVCA
                     LVFSIRFYEAVGTLFQALMLALLAGMVGFAFSADLFDLFVFLELVAATTYALVAYRIQ
                     ERGPLEASLNTAVTNTVATLVLLIGIALVYGRTGALNLGQVFRAFDGRSADLLVTMAL
                     VASAVGFLVKAAAVPFDMWLADAYAVAPIPGGVIFAGVLTEMGLYGLGRIRWTAFAGI
                     GQIARFDLVILAVGAITAVVGALMAVPQRRLKRLVAFVAVAHSGVLLMGLGTGTSEGL
                     AGTALYLLADGGLKAALFLAVGILKHRRGSIHEDRLIGRGRDLHVAGIVFLLTGLGIA
                     GLPPFGTALGKGLIEAAALEHGMSWAPWVLGLTSVLTGGAILRAAGRIFLGWGPEEAT
                     SHADEEEVTETEGGRGGTPLVMSGPAAALVVLALAIGCVPRLLHAAHVGALQFREPAR
                     YAAALFGSRTAPVSIPPAPPWWDAPTLLSGVLAAVAAVAFALVALDRVRIPARLTRVA
                     TRLWTSPMARLRAAHSGHVGDSIAWLVGGAASLGIAVSLILGVFR"
     gene            complement(1843..2496)
                     /locus_tag="VE646_04955"
     CDS             complement(1843..2496)
                     /locus_tag="VE646_04955"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HZD79370.1"
                     /translation="MGAGAGVLGLLQELARWLGPVFTTAGYAIVPAVVFLETAALIGW
                     VVPGDVTMALAGVYAARGDLALEWILTSGVVAAIAGQTGGYWLGRRYGRSIIQRVPFV
                     RRVEPRMEEASAFLRRHGGKAVMLGRFATGVSSFVPFTAGTAGMPFPRFLAFMIPTSL
                     VWVSAVVLLGYLLGRNLALIDRVLSGVGWVVLAVVLLLLGGAWLWHRRRKQGGTDRR"
     gene            complement(2504..3289)
                     /locus_tag="VE646_04960"
     CDS             complement(2504..3289)
                     /locus_tag="VE646_04960"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HZD79371.1"
                     /translation="MAGLAERFPRTRRLIGRLSGHQILFSASGLAFYALISLAPVVIL
                     VMWIASLLLGDQRVHHFADAVGRMAPNDLGLDSALRSVAQRGTSIGVLAGVTALWPAS
                     AYGAGLTRAFDRVADGSYELPGLRGRVLLIVVLLPLSTVGALVVSYLGTTLVDPSLFH
                     GVAAGLVALGVGFVWIFVSTLLIYRVFPPERLPWNAILRATLIAAGGAAALSALLALF
                     LAAGTNFKEHYATSAVASLVLIGIWLYLSNALLLAGFRLVDTD"
     gene            complement(3363..3755)
                     /locus_tag="VE646_04965"
     CDS             complement(3363..3755)
                     /locus_tag="VE646_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79372.1"
                     /translation="MADETWIWEIRNPDGGSMGLELARGRMAPGPRVLAHSLPERADV
                     TVVDEEGELVARGRDLRDDGGTPMAELSVDGGSVRRRNRWPTEEDLGTPVILPGGEVG
                     LLERWWNAEDGSEWRWSVGFHNRVAPGG"
     gene            3839..4858
                     /gene="galT"
                     /locus_tag="VE646_04970"
     CDS             3839..4858
                     /gene="galT"
                     /locus_tag="VE646_04970"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HZD79373.1"
                     /translation="MSTLRQDPTTRQWVILAPRRGARPHQELEGPSRDDLPSFDAGCP
                     FCPGNEDQTPPEILREPSGADWQVRVVPNLYGALSGDGPAVRSGPPMFRELPGVGGHE
                     VVIESPAHDGRMDEMSIPDVTRILRVWRRRYRDLMAAPHVRAVVVFKNFGALAGTSLV
                     HTHSQVVATPVFLPRLLHRLDVATRHFDDHGSCVYDDVIAAEREAELRVVEGCGRFLA
                     FVPFAAGSPFEVWIAPTYHEGSFGEIRDEDVDDLSCLLIRTLAAIRKACADPDFNLVV
                     YSSPANGHPAEVFHWHIKVLPKLSTPAGFEIGSGMSIQTVPPEDAALALRGALSTIPM
                     PARPA"
     gene            complement(4880..6310)
                     /gene="gltX"
                     /locus_tag="VE646_04975"
     CDS             complement(4880..6310)
                     /gene="gltX"
                     /locus_tag="VE646_04975"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZD79374.1"
                     /translation="MRCRFAPAPSGSLHVGNVRTGLFSWAFARHHRGAFILRVEDTDA
                     SRVTEEAVHGVIESLRWLGVDWDEGPDVGGPHQPYRQSQRGEVYRDAVERLLAKGAAY
                     PCYCTPDELEERRRAARARGRAWTYDGRCRNLSQAERATFEAEGRASAIRFAMPEREW
                     VVQDLVKGEVRFAPGALGDFIMVRSDGSPTFLLAVAVDDLRMEVTHVIRGDDLLASAP
                     RNAAVIEALGGTAPRYAHVPQVLGGDGKPLSKRHGSTSVEAFREQGVVHEALLNYLAL
                     LGWSKDERTTFLSKQELVEAFELERVSSNPAAFDTKKLEWMNNHYIQQLDDDDLAARC
                     MPFLTQAGLLPEPELLRRAMPLVRERMSTLRGSVDMLRFLFTDDLVPNEQARGLLERA
                     GPEYLRAGARALEAVEPWTAERIGTALDALAETNGLNRRKGWQPIRAAVTASDVSPPL
                     PQSLELLGKERAVARVQASAGSLAAG"
     gene            complement(6313..7074)
                     /locus_tag="VE646_04980"
     CDS             complement(6313..7074)
                     /locus_tag="VE646_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZD79375.1"
                     /translation="MRLVRFRTADRIATGALEGGSGSVRVLGGTFFQNPVPTGEELPL
                     DDVRLLAPVLPSKVVAVGRNYLAHAKEFGGAVPDEPLLFFKPSTAVIGPGDPIPLPAV
                     SRRVDYEGELAVVIGRLARNVAAEDAYRVILGYTCGNDVTLRDLQRNDDQWARAKGFD
                     GSCPLGPWIETDLDPTGVRIETRLNGDTRQFATTADMAFGVATLVEYITSFLTLLPGD
                     VILTGTPEGVGQLSPGDVVEVEVEGLGTLSNPVAS"
     gene            complement(7086..>7980)
                     /locus_tag="VE646_04985"
     CDS             complement(7086..>7980)
                     /locus_tag="VE646_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD79376.1"
                     /translation="VAAALERVGLVPVEQFLHKYPGDLSGGQKQRAVLARAIILDPDL
                     LIADEPVSMLDMSVRAKILELMLQLKTELDLTYLYITHDLATAKFFCDRIAIMYLGQI
                     VEIGPTDAIFEDPKHPYTVALLKAVPDPDPDRMVPRDLPRGEVPDAANPPLGCRFHPR
                     CPRAFAVCGWESRDLKEHIEEHWARGSKDRFEQERAILGDLGALAAPTTTARVPAGRA
                     HDGEHVRALLDRMREEDPDRPLWTGVAEVRSEAAACTVSFREPLEPRLLDLDPVTVAC
                     HLYDPDARRAAEEGGGSGAMR"
BASE COUNT         1086 a         2966 c         2795 g         1133 t
ORIGIN      
        1 gggggtgaac cggacctgga cgtcctgctg ccggttgccg tcgcgctccc gctgttcggc
       61 gccgccgcgc tggcagccct cacgaaactg cttccccgac gaccggcgga cctgctggcg
      121 ctgggcttcg cctgcgccac caccgcgatc tgcgccctgc tgacgatcgt cgccgcctcc
      181 gggccggtga tccactggtt cggtggctgg acgccgctgg gccggcaggc agtgggggtg
      241 gccttcctgg ccgacggctt cgccgccggc ttcgcggcgt tcatcggcct gctcgtggtc
      301 tgcgccctgg tgttctcgat ccgcttctac gaggcggtgg gtaccctgtt ccaggcgctg
      361 atgctcgcgc tgctggccgg gatggtcggg ttcgcgttct cggccgacct gttcgacctc
      421 ttcgtgttcc tggagctcgt cgcggccacc acctacgcgc tggtggccta ccggatccag
      481 gagcgcggtc ccctggaggc ctccttgaac accgccgtca cgaacacggt cgcgacgttg
      541 gtgctcctca tcgggatcgc cctggtctac ggacggaccg gggcgctgaa cctggggcag
      601 gtcttccgcg ccttcgacgg ccgctctgcc gacctgctcg tcaccatggc cctggtggcc
      661 tcggcggtgg ggttcctggt gaaggcggcg gcagtgccct tcgacatgtg gctggcggat
      721 gcctacgccg tggccccgat ccccgggggc gtgatcttcg ccggcgtcct gaccgagatg
      781 ggcctctacg gcttgggtcg gatccgctgg acggccttcg caggcatcgg ccagatcgcc
      841 cggttcgatc tggtcatcct ggccgtgggg gcgatcacgg ccgtggtggg agcgctcatg
      901 gccgttcctc aacgccgcct gaagcggctg gtcgccttcg tcgcggtggc gcattcgggc
      961 gtgctgctga tggggctcgg gaccgggacc tcggaggggc tggcgggaac ggccctgtac
     1021 ctgctggccg acggcggact gaaggccgcc ctgttcctgg cggtggggat cctgaagcac
     1081 cgacgaggtt cgatccacga ggaccggttg atcggtcggg ggcgggacct gcatgtggcg
     1141 ggcatcgtct tcctgctcac gggcctgggg atcgccgggc tcccgccgtt cgggaccgcg
     1201 ctgggcaagg ggctgatcga ggcggccgcg ctcgagcacg gcatgtcgtg ggcaccgtgg
     1261 gtgctcggcc tgacctcggt cctaacggga ggcgcgatcc tgcgggccgc cgggcgcatc
     1321 ttcctggggt gggggccaga ggaggcgacg tcccacgccg atgaggagga ggtcaccgag
     1381 accgaagggg gccgaggggg gacgccgctg gtgatgagcg ggccggcggc ggcgctggtg
     1441 gtcctggccc tggcgatcgg gtgcgtcccc cggctgctgc acgcggccca cgtgggggcg
     1501 ctgcagttcc gcgagcccgc ccggtacgcc gcggcgctgt tcgggagcag gacggccccc
     1561 gtgtcgatcc cgcctgcgcc gccctggtgg gacgccccga cgctcctgtc cggggtcctg
     1621 gccgcggtcg cggcggtggc cttcgccctg gtggccctgg accgggtccg gatccccgca
     1681 cggctcacgc gcgttgcgac ccggctctgg acgagcccga tggctcggct ccgagcggcc
     1741 cacagtggcc acgtggggga ttcgatcgcc tggctggtgg gcggggccgc gtccctgggg
     1801 atcgccgtct ccttgatcct cggcgtcttc cgctgatccg ggtcaccttc ggtcggtgcc
     1861 accctgtttg cgccgacggt gccacagcca ggcccccccg aggaggagca gcaccaccgc
     1921 cagcaccacc cagcccaccc ccgacagcac ccggtcgatc agggcgaggt tccggccaag
     1981 gaggtacccg agcaacacca ccgcgctcac ccacaccagg gaggtgggaa tcatgaaggc
     2041 gaggaagcgg gggaacggca tcccggccgt gccggcggtg aagggcacga aggagctgac
     2101 gcccgtggcg aatcggccca acatgaccgc cttgccgccg tgccgccgca ggaacgccga
     2161 ggcctcctcc atccgcggct ccacccggcg cacgaacggc acccgctgga tgatcgagcg
     2221 gccgtagcgc cgccccagcc agtagccgcc cgtctgtccg gcgatcgccg ccaccacgcc
     2281 cgaggtgagg atccactcga gggccagatc gccgcgggcc gcgtacacgc cggccagcgc
     2341 catcgtcacg tcccccggga ccacccaccc gatcagggcc gcggtctcca ggaacaccac
     2401 cgccgggacg atcgcgtacc cggcggtcgt gaagacgggt ccgagccatc gggccagctc
     2461 ctggaggagc cccagcacac cggcaccggc ccccacgcct gcctcagtcg gtatccacca
     2521 gccggaagcc ggccagcagc agcgcgttcg acaggtacag ccagatgccg atcagcacca
     2581 gggacgccac ggcgctggtg gcgtagtgct ccttgaagtt cgtccccgcg gccaggaaca
     2641 gcgcaagcag ggcggacagc gccgccgccc ctccggccgc gatcagcgtg gcccgcagga
     2701 tggcgttcca cggcagccgt tccggcggga agacccggta gatcagcagc gtcgagacga
     2761 agatccacac gaagcccacc cccagcgcca cgagcccggc agccacaccg tggaacaggg
     2821 acgggtccac cagggtcgtc cccaggtacg agaccaccag ggcgccgacc gtggacaagg
     2881 gcagcaggac cacgatcagc agcacccggc cccgcaaccc ggggagctcg tacgagccat
     2941 cggccacccg atcgaaggcc cgggtcaggc ccgccccgta cgcgctggcc ggccacaggg
     3001 cggtgacgcc cgcgagcacg ccgatgctgg tgccccgctg ggccacgctc cgcagggccg
     3061 agtccagtcc caggtcgttc ggggccatcc tgccgaccgc gtctgcgaag tggtggacgc
     3121 gctggtcgcc gaggagcagg ctcgcgatcc acatcaccag gatcaccacg ggcgccaggg
     3181 agatgagcgc gtagaaggcc aggcccgagg cggagaacag gatctggtgg ccgctcagtc
     3241 gaccgatcag ccggcgcgtc ctggggaagc gctcggccag cccggccacc gctcgggaac
     3301 cgacctcgcg caggccctcc cccagctccg cgccgttgcg ttgttccgtc tgcgccagcg
     3361 ggctaacccc ccggcgcgac gcggttgtgg aacccgacgc tccagcgcca ctcggagccg
     3421 tcctccgcgt tccaccaccg ctcgagcaga cccacctcgc cgccgggcag gatcaccggg
     3481 gtgcccaggt cctcctcggt cggccaccgg ttgcgccggc ggaccgagcc tccgtccacc
     3541 gacagctcgg ccatgggggt gccaccgtca tcccggaggt cgcgccctcg ggccacgagc
     3601 tcgccttcct cgtccacgac ggtgacgtcc gcgcgctcgg gcagcgagtg ggccagcacg
     3661 cgcggccccg gtgccatccg cccgcgcgcg agctccaggc ccatggagcc cccgtcgggg
     3721 ttacggatct cccagatcca ggtctcgtcc gccatcgagg gaccccttcc cggagcgcgg
     3781 gggggacaaa ccgggcgccc gtccaccccg gcccggttcc tgtaccgtgg tccctcgtat
     3841 gtccaccctc cgacaggatc cgaccacccg gcagtgggtg atcctcgccc cccggcgggg
     3901 cgcccggccg caccaggagc tcgagggccc gtctcgggac gatcttccga gcttcgatgc
     3961 cggatgcccc ttctgccccg gcaacgagga ccagacccca ccggagatcc tccgggagcc
     4021 ctccggtgcc gactggcagg tgcgcgtggt cccgaacttg tacggggcgc tctccggcga
     4081 cggccccgcc gtccgcagcg gccccccgat gttccgggag ctgcccggcg tgggcggtca
     4141 cgaggtggtg atcgagtccc ccgcgcacga cgggcggatg gacgagatgt ccatccccga
     4201 cgtgacccgg atcctccgcg tgtggcggcg gcggtaccgg gacctcatgg ccgcccctca
     4261 cgtccgggcg gtggtcgtgt tcaagaactt cggagccctg gccgggacgt cgctcgtcca
     4321 tacgcattcg caggtggtgg ccaccccggt gttcctgccc cggctgctgc accggctcga
     4381 cgtggccacc cgccacttcg acgaccacgg gagctgcgtc tacgacgacg tgatcgctgc
     4441 cgagcgcgag gccgagctcc gggtggtgga gggatgcggc cggttcctgg ccttcgtgcc
     4501 cttcgccgcc ggctccccgt tcgaggtgtg gatcgctccc acctatcacg agggatcgtt
     4561 cggcgagatc cgtgacgagg acgtggacga cctgtcctgc ctgctgatcc ggaccctggc
     4621 cgcgatccgc aaggcctgcg ccgaccccga cttcaacctg gtggtgtact cctcccccgc
     4681 caacggccac ccggcggagg tcttccactg gcacatcaag gtcctgccga agctctccac
     4741 ccccgccggc ttcgagatcg gctccggcat gagcatccag acggtccctc ccgaggacgc
     4801 tgcgcttgcc ctgcgggggg ccctctcgac gatcccgatg ccggcccgtc cggcctgagc
     4861 ggacggcctc ggcgaccgct cagccggcgg ccagggaccc ggccgaggcc tggacccggg
     4921 ccacggcgcg ctccttgccc aacagctcga gggactgcgg caagggcggc gagacgtcgg
     4981 acgcggtgac cgcggcccgg atcggctgcc agcccttcct tcggttcagg ccgttggtct
     5041 ccgccagggc gtcgagcgcg gtcccgatcc gctcggcggt ccacggttcg accgcctcca
     5101 gcgcccgggc gccggcccgc aggtactcgg gaccggctcg ctcgagcagg cctcgagcct
     5161 gctcgttcgg caccaggtcg tcggtgaaca ggaaccggag catgtccacc gacccccgca
     5221 gggtgctcat gcgctcccgc accaggggca tggcccggcg gagcagctct ggctcgggca
     5281 ggagccccgc ctgggtcagg aacggcatgc accgggcggc caggtcatca tcgtcgagct
     5341 gctggatgta gtggttgttc atccactcca gcttcttcgt gtcgaaggcg gccgggttgc
     5401 tggagacccg ctccagctcg aacgcctcca ccagctcctg cttggacagg aaggtggtcc
     5461 gctcgtcctt ggaccacccc agcagcgcga ggtagttcag cagcgcctcg tgcactaccc
     5521 cctgctcgcg gaaggcctcc accgaggtcg acccgtgccg cttggagagt ggcttgccgt
     5581 caccgcccag gacctgcggc acgtgggcgt accggggcgc cgtgcccccg agggcctcga
     5641 tcaccgcggc gttccggggc gcgctggcca gcaggtcatc gccccggatc acgtgggtga
     5701 cctccatccg caggtcgtcc acggccaccg ccagcaggaa cgtgggcgag ccatcggagc
     5761 ggaccatgat gaagtcgccg agggcgccgg gggcgaagcg cacctcgccc ttgaccaggt
     5821 cctggaccac ccactcgcgc tcgggcatcg cgaagcggat cgccgaggct cggccctcgg
     5881 cctcgaacgt ggcgcgctcg gcctgggaca ggttccggca ccggccatcg taggtccagg
     5941 cccgtccccg ggcccgagcc gcccgccgcc gctcctccag ctcgtcgggg gtgcagtagc
     6001 aggggtaggc cgctcccttg gccaggagcc gctcgacggc gtcccgatag acctcgccgc
     6061 gctgcgactg ccggtacggc tggtggggtc cgcccacgtc gggtccctcg tcccagtcca
     6121 cccccagcca gcgcagcgac tcgatcacgc cgtgcacggc ctcctcggtg acccggctgg
     6181 cgtcggtgtc ctccacccgc aggatgaagg cgccccggtg gtgccgggcg aaggcccagc
     6241 tgaacagccc ggtccgcacg ttgcccacgt gcagggagcc ggagggggcc ggcgcgaacc
     6301 ggcaccgcac gttcacgagg cgacggggtt ggacagcgtc cccagcccct ccacctcgac
     6361 ctcgaccacg tctccggggg acagctgacc gacaccctcg ggcgtgccgg tgaggatgac
     6421 gtcgccgggc agcagggtca ggaacgaggt gatgtactcg accagcgtgg ccaccccgaa
     6481 ggccatgtcg gcggtggtgg cgaactgccg cgtgtccccg ttcaggcggg tctcgatgcg
     6541 caccccggtg gggtccagat cggtctcgat ccacggcccg agcggacacg agccgtcgaa
     6601 gcccttggcg cgggcccact ggtcatcgtt gcgctgcaga tcgcgcagcg tgacgtcgtt
     6661 cccgcaggtg tacccgagga tcacccggta cgcatcctcg gccgcgacgt tgcgggccag
     6721 ccggccgatc accaccgcca gctcgccctc gtagtcgacc cggcggctga cggccggcag
     6781 cgggatcgga tcccccggcc cgatcaccgc ggtggagggc ttgaagaaca ggagcggctc
     6841 atcgggaacg gcgcccccga actccttcgc gtgcgccagg tagttccgcc ccaccgccac
     6901 gaccttcgag ggcaggaccg gcgccagcag ccgaacgtcg tcgagtggca gctcctcgcc
     6961 ggtgggcacc gggttctgga agaacgtgcc gccgagcacg cggaccgagc cggacccccc
     7021 ctccagggcc ccggtggcga tgcggtcggc ggtgcggaat cggacgagcc tcatcgggcc
     7081 cgaggctacc gcatcgcgcc ggacccgccc ccctcctcgg ccgcacgcct ggcgtcggga
     7141 tcgtacaggt ggcaggccac cgtcaccgga tccaggtcca gcagccgggg ctccaggggc
     7201 tcgcggaacg acacggtgca ggcggccgcc tccgaccgca cctcggccac cccggtccac
     7261 aggggccggt ccgggtcctc ttccctcatc cggtccagga gcgcacgcac gtgctccccg
     7321 tcgtgggcgc ggccggcggg cacccgtgcc gtggtggtgg gcgccgccag cgcccccaga
     7381 tcgcccagga tggcgcgctc ctgctcgaac cggtccttgg agccgcgggc ccagtgttcc
     7441 tcgatgtgct ccttcaggtc ccggctctcc cacccgcaga ccgcgaacgc ccgggggcaa
     7501 cgggggtgga accggcagcc gagcggagga ttcgcggcgt cggggacctc gcccctgggg
     7561 agatcgcgcg ggaccatccg atccggatcc ggatccggca cggccttgag cagggccacg
     7621 gtgtacggat gcttgggatc ctcgaagatc gcatcggtgg ggccgatctc cacgatctgg
     7681 ccgaggtaca tgatcgcgat ccggtcacag aagaacttcg cggtggccag gtcgtgcgtg
     7741 atgtacaggt aggtgagatc cagctcggtc ttcagctgga gcatgagctc gaggatcttg
     7801 gcccgcacgc tcatgtccag catcgacacc ggctcgtcgg cgatcagcag gtccgggtcc
     7861 aagatgatgg cgcgggccag caccgcccgc tgcttctgcc cgccggacag gtccccgggg
     7921 tacttgtgca ggaactgctc cacgggaacc agccccaccc gctcgagtgc cgcggcgacg
//
LOCUS       DASQQQ010000118        17004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_340133, whole genome shotgun sequence.
ACCESSION   DASQQQ010000118 DASQQQ010000000
VERSION     DASQQQ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 17004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17004
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340133"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2364)
                     /locus_tag="VE646_04990"
     CDS             complement(<1..2364)
                     /locus_tag="VE646_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZD79377.1"
                     /translation="MDRPRCERCGEPLPVDARFCPNCGFPTTAPPPEERKVVTVVFTD
                     LVGSTQLSSRIDPEAYREVIAAYYQAVTEELESLRGRAYNFAGDAVVGVFGLPQAHDD
                     DALRAIRAGLAVVDRVGRLGQSLGLPVPLRCRVGINTGSVAIGSEAAEQGLLFGAAVN
                     AAARLQQGAEPGGLLVGETTRMIALERVEFGERRLVQAKGFEEQLPGWPVVALAPRST
                     RRTIPFVDRRRELRLLRDAFERAVESRRGHMVTLVGEPGIGKSRVIDEFVAALPAQVH
                     VLTGRADPFEEDVTFAPVGQMLLQELGERPDAPKDLLQARLEEVVLSCCSADRSEQIV
                     ARLGHVLSLVDDPQEDRRSRVAEVRSGLLAFLEGLGAREPVVLVFEDLHLAEPHLLDL
                     VEHTVRDARGMPLFVLCAARDDLLDQRPDWGGGLRDSLTLFLESMSVDDATELALQAG
                     EGLDPRTAERIARHAGGNPFFIVETTGMLLHVGGELPSDTGPLPQALLPPTVQAVIAA
                     RIDHLPGEARDVVRKASVFARSTFDLSELRLIAEAGEPILRLLEEEELLLPDAERAGE
                     WRFRHGMVRDVAYESLPKRERRRLHLSVADRLAEDPHTAARYPRSIAYHLEQAARASL
                     DLNPADRALAERAVDALAHAGHLALDGSEPRPAADLYERAIALAGPEAGWGRREADAL
                     AHLGESRYWLGEFEAAVPPLTRAIELATEDLPIRAQASRFLGDIELSIHGNQERAEAL
                     LDRSLAAARELGDPHTLARTLLTAGWAPYWRNDLEGAGAMFEEALNIA"
     gene            complement(2414..2899)
                     /locus_tag="VE646_04995"
     CDS             complement(2414..2899)
                     /locus_tag="VE646_04995"
                     /inference="COORDINATES: protein motif:HMM:NF023594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HZD79378.1"
                     /translation="MREKIARPELVRRWEGDFPVGHRYTPGVAGDAFFTALRDRGVLL
                     GSRCEGCDYTYVPARLFCERCFSELAADTEVGPGGELVSFTIGFVGPEERPLDLPETI
                     GLIRPDGADAVLVHRILDPGDEPLEVGARVQMILREAERRVGSILDIEGFRIVGEPSV
                     P"
     gene            complement(2899..3543)
                     /locus_tag="VE646_05000"
     CDS             complement(2899..3543)
                     /locus_tag="VE646_05000"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HZD79379.1"
                     /translation="MQVGTVVVMSRDGASAAGPPGGASLVPPASRAPEPPAAPGAEPV
                     TVVQTPPFAMGGHPLSDAEFRSATGAVDDAIEARYAWDAGVAVGRFLDGLAEGRILGR
                     VCHGCGRVLVPPRMFCERCFRETDGWAEVEPSGVVQTYSVCHVSWDMQRIEPPELPAV
                     IAIDGSSGGFLHKLGEVAPDDVRIGMAVEAVWKPQAERTGSILDIAYFRPVAEG"
     gene            complement(3517..4671)
                     /locus_tag="VE646_05005"
                     /pseudo
     CDS             complement(3517..4671)
                     /locus_tag="VE646_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014512167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
     gene            complement(4671..5027)
                     /locus_tag="VE646_05010"
     CDS             complement(4671..5027)
                     /locus_tag="VE646_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZD79380.1"
                     /translation="MSVNTLDHVWFWVGDMDRAVVFYRDVLELGLMRRDGDEWAEFDA
                     GAVRLALHGAGPGSAPGGTAVFRVDDLVTARARLEERGVTFDHAGEVDGYARYATFRD
                     PFGNGLQLIEFIEGGG"
     gene            complement(5024..6193)
                     /locus_tag="VE646_05015"
     CDS             complement(5024..6193)
                     /locus_tag="VE646_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HZD79381.1"
                     /translation="MEPRVAVVGIGHSGFAPITAGLSYKELMFEAAQRAYDDAGVDPR
                     TDIDSFVCCSEDLLEGTSIFDEYVPDQLGALHRPVQTVAGDGLFGVATALMLIGAGAA
                     RVVAVEAHSKVSDVLTLGRIERFALDPVLNRPVVPSVLAIAGLDMTAFLERTGLSEEH
                     CAIVAAKNRRNALDNPRAAYPSDLGADEVDDSAPSYWPLRELEIAGPADGCVVVVLAA
                     EELVRDLTEAPAWILGTGWCSGSPTLESRRWGEADTVRAAAERAYAMAGIEDPREQID
                     LAELDDTYAYKELEHLEALGLVDGEELPQLLADGELEPDGDLPVNVSGGALGQGNLFQ
                     ANGLVRLQECVEQLRGEAGERQVDEVFTALAQSWPGVPSTSSAVMVLANDEGVGG"
     gene            complement(6246..6881)
                     /locus_tag="VE646_05020"
     CDS             complement(6246..6881)
                     /locus_tag="VE646_05020"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZD79382.1"
                     /translation="MAFPRRLLVEGEELILDLRPHWIALVGPAAVAILGLAAYIVLLV
                     YAPSSGFGRSVLVWGGLIALFLLLVWYPARRAVRWATSHFVVTSDRVIHRQGWIAKSS
                     MEIPLEAINDVRFSQGVFERIIGAGDLSIESAGERGTNVFSDIRNPEEVQKTIYHQGE
                     MNQQRMMRGGPAPGAPSTTGELERLASLRDRGVLTQEEFEAQKRRILGAGR"
     gene            complement(6931..7683)
                     /locus_tag="VE646_05025"
     CDS             complement(6931..7683)
                     /locus_tag="VE646_05025"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HZD79383.1"
                     /translation="MPLSEHALTHALERIGVQAPVRFDEITRSTQLTAVELARQGAPE
                     WTLVGSNHQTEGRGRLGRSWVDEPDRGLIVSVVLRPQLHPEQGGLLTLLAGWAMATAC
                     RLSGTPVGCRWPNDLVTREGKVGGILAESLLEQDRFAFVVLGTGVNIGQAPYVHHAAA
                     IEAVDPADLLEDFLRTFVRRYEPTHPAFAGAVTSSYRDVCVTLGNEVSTTTVSGARIE
                     GRAVDVDQAGGLVVSTQAGPEVVRFGEIRHLR"
     gene            7734..9458
                     /locus_tag="VE646_05030"
     CDS             7734..9458
                     /locus_tag="VE646_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HZD79384.1"
                     /translation="MPKTRILVANRGEIAVRIIRTIRELGYRSVAVYSDADRDALHVE
                     MADSAYRIGPPLPADSYLSIGAILEAARKAKATAVHPGYGFLSERAHFATAVEESGFL
                     FIGPPAEAIDMMGDKSAARQAADRVGVPIVPGTTEPVTVKQARRHAERIGFPLLVKAA
                     FGGGGRGMHIVRTGADLEPALTRAAREAKAYFGRPEVFLERYIERAHHVEAQILADRH
                     GTISFLGERDCSVQRRHQKLVEETPSPVVDAELRSRIGEAAMMLAKEAGYTNAGTVEG
                     VVDDEGHFYFLEMNTRLQVEHTVTEMVTGLDIVALQIAVALGEHVDLSSVRGRGHAIQ
                     CRINAEDPSKNFLPGPGRVAGYREPSGPFVRVDSYLRPGKEVPSDYDSMFAKVIVSAE
                     DRERARRRMLRALDEFQVEGIPTTIPLHRWVLGTREFREGTHTTAWLEPALRDLKLPM
                     PEIALPPTEAKQPVPAELLVELDGRRVPVRIFDQRRDTAPRPPSRHLSTHGEHVHSVV
                     AAPMQGTILRVLVEQGQEIAAGDVLCIVEAMKMENAIPAPRDGMVSDLPVQAGQVVEP
                     GQTLAVID"
     gene            complement(9473..10165)
                     /gene="npdG"
                     /locus_tag="VE646_05035"
     CDS             complement(9473..10165)
                     /gene="npdG"
                     /locus_tag="VE646_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HZD79385.1"
                     /translation="MDVAVIGGTGEEGFGLTLRLARAGQHAIIGSRSQEKGATTAEKA
                     RGILGADGRVDGTTNQAAAEAGEIVFVTVPYAGQADIYRAIKDHVAAGKIVVDCTSPL
                     ATAVGGRAWQVVHPWHGSAAEQAKAILDPGVRMVAAFHTIAGGALQDLEHPIESDVLV
                     CGRDREAKGLVGGVIDQIPDLRWVDAGDLSMARIVETLTAVLVSVNRNYRIHDSGFRM
                     TGRDSWGPRPER"
     gene            complement(10248..10931)
                     /locus_tag="VE646_05040"
     CDS             complement(10248..10931)
                     /locus_tag="VE646_05040"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HZD79386.1"
                     /translation="MRSTRRLLGVAVAGSIAMSLLAAAPAGAAGGAKATLNVVHGIPG
                     ADVNVCVNGAEAISDFNPGEVVKGVKLPPGSYDLKVVAASDDCADPAVLEADGIRLKE
                     GRNYTAVAHLKANGAPTIGLFRNDMSEVGEGRARLIVRHTAAAPAVDLWANGAILGEG
                     LNNGETFTAVLPRGVYAAWVSLPGDYRPVIGPDVLKLRRGHTYQVYAWGNGTDGYDLA
                     VVPARSGVK"
     gene            11120..11281
                     /locus_tag="VE646_05045"
     CDS             11120..11281
                     /locus_tag="VE646_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79387.1"
                     /translation="MARTMTYLTELVVGVVCLGAGTGLIRRGPRMAGLLLGVAGVAAV
                     VHAMVRLPG"
     gene            complement(11294..12913)
                     /locus_tag="VE646_05050"
     CDS             complement(11294..12913)
                     /locus_tag="VE646_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZD79388.1"
                     /translation="MDQRDRSSGAEPSRQRERHITDSGIRVRPVYGPDDLAGFDPGRD
                     LGAPGEPPYTRGVYPTMYRGRLWTMRQYAGMATAQETNRRFRYLLEQGQTGLSVAFDL
                     PTQMGYDSDHPRAEGEVGKTGVAICSIEDMHRLFEQIPLDRVSTSMTINATAAVLLLL
                     YELVAEERGIDPGQLSGTVQNDLLKEYAARGTYIYPPKPSMRIITDLFAYCRGRIPRW
                     NTISISGYHMREAGATAVQEVAFTLANGIAYVQAALAAGLAVDDFAPRLSFFFACHMN
                     FFEEIAKFRTARRLWARIMRERFGAVDARSAMLRFHTQTGGATLTAQQPETNVVRTAF
                     EALAAVLGGTQSLHTNSFDEALALPTEHAAKLALRTQQVLAYETGVPDTVDPLAGSYF
                     LETLTDQLQERVERYLEKIEGMGGAVAAIEQGFYQDEIHEAAFKIQRGIEAGNRVVVG
                     VNRFLDPHERPVEIQRIGEEEVARQVERVRALRAGRDRAAVDASLAAVGAAARGSANL
                     LPPMKEALRARATLGEVSDVLREAFGEYRPR"
     gene            13017..13913
                     /gene="nadC"
                     /locus_tag="VE646_05055"
     CDS             13017..13913
                     /gene="nadC"
                     /locus_tag="VE646_05055"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018858110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HZD79389.1"
                     /translation="MVVVVSSTSLTSPHLGASAADDLVRRALAEDAPSGDPTTALVIP
                     AGTSCSAELRAKQAGIVAGMPVARMVFETAVEQDGAGSIGFTARVRDGAEVRPDEVLA
                     VVRGPARTILRAERVAINLLSHLSGVATLTRAFVDAAAPARILCTRKTTPGLRELERA
                     AVVAGGGSLHRASLSDAVLIKDNHVRIAGGVGTAVRRARREGHPVEVEVETLDQLDEA
                     LAEGAERILLDNATPDLVRRAVDRVGDAGRLEISGGVRLETVGDLVSAGARVISVGRL
                     THSAPALDISLEVIRGDEDARG"
     gene            13888..14922
                     /gene="nadA"
                     /locus_tag="VE646_05060"
     CDS             13888..14922
                     /gene="nadA"
                     /locus_tag="VE646_05060"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HZD79390.1"
                     /translation="MGTKTRAAEGSEALAERFTSWADEVRRLAQERRAVILAHNYQVP
                     WIQDVADFVGDSLALSRRAAATDAEVIVFCGVHFMAETAKVLSPDKTVLLPDLDAGCS
                     LADTVTAEQVRTWKAEHPGAAVVAYVNTTAEVKAEADICCTSSNASDVVGSIPEDREI
                     LFLPDMFLGAHVERVTGRKMHVWMGECHVHAAIDPEQLERKALEDPDAAFMIHPECGC
                     TTSALWLLSEGVLPAERTRVLSTGGMVAEARRSSSDRFLIATETGILHQLRKAAPQKI
                     FDPIDEDAVCRYMKAITPEKLLRSLREGVFEITVPQAVAARARRAVERMIEVGPSSGP
                     VSPAPSNRSR"
     gene            14928..16460
                     /locus_tag="VE646_05065"
     CDS             14928..16460
                     /locus_tag="VE646_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZD79391.1"
                     /translation="MRTVVVGAGAAGLFCALHAAERGPVSLVAPAAALDAATAWAQGG
                     IAAATGPEDSPELHAADTIAAGRGLCDPVAVAVLAREAPWAVDELRALGMPFDRGATA
                     LEGGHRARRVLHAGGDASGRELHRFLADRVRDDERVERVHGLVRAVVRDLEGARGVRL
                     ADGTVIEGDRVVLATGGACGIFGHRTGPETSTGDGLVLAGDAGAALADLEFVQFHPTA
                     LDAPGHPARLLTEALRGEGALLTDAEGRSFMHRFHPSGDLAPRDVVARAVVRVREETG
                     RPVFLDARSVPDVASRFPVAAEQCREVGLDIAARPIPVAPAAHYFMGGVLTDTWGRTS
                     VPRLFACGEVAATGVHGANRLASNSLAEALVFGRRVALAEDGSWEVTDRGTRDAPQPP
                     ARPGGPGLAELRALCDEHLGVQRSGPDLDRFLAALDPADRSRDGSAARVARLMALAAR
                     RRTESRGGHFRVDFPGIDPAWRVRQAVDRDGWWALPVPDPRPSDGVPREERPGSSSVV
                     PQ"
     gene            16533..16931
                     /gene="sdhC"
                     /locus_tag="VE646_05070"
     CDS             16533..16931
                     /gene="sdhC"
                     /locus_tag="VE646_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HZD79392.1"
                     /translation="MAKRKTDVGTLYKGSPGQWSWLAHRITGIAVILFLFAHVVDTAV
                     VGWGPGAYDRVIQVYENWAVRILELGLVAAVMYHAINGVKIMIFDFWPSSTRYFKTIS
                     LISTGIFLAGMVPITWIMGKDIIHLAFGGA"
BASE COUNT         2407 a         6255 c         5976 g         2366 t
ORIGIN      
        1 ggcgatgttc agggcctcct cgaacatcgc tccggcccct tcgaggtcgt tgcgccagta
       61 cggggcccac cccgccgtga gcaacgtccg ggccagcgtg tggggatccc cgagctccct
      121 ggccgcggcc agcgatcggt cgagcagcgc ctcggcccgc tcctggttgc cgtggatcga
      181 cagctcgatg tcgcccagga accggctggc ctgggcccgg atcggcaggt cctcggtggc
      241 gagctcgatc gccctggtga gcgggggcac cgccgcctcg aactcgccca gccagtaccg
      301 tgactcgccc aggtgggcca gcgcgtcggc ctcgcgccgc ccccaccccg cctcgggacc
      361 ggccagcgcg atagcccgct cgtacaggtc cgccgccgga cgaggctcgg agccgtccag
      421 cgccaggtga ccggcgtgcg cgagggcatc caccgcgcgc tcggccagcg ctcggtcggc
      481 cgggttcagg tccaacgaag cgcgggccgc ctgctccagg tgataggcga tcgagcgcgg
      541 gtaccgcgcg gcggtgtgtg ggtcttccgc cagccggtcg gccaccgaca ggtgcaggcg
      601 ccgacgctcc cgtttgggca ggctctcgta cgcgacgtcg cgcaccatgc cgtgccggaa
      661 ccgccactcg ccggcccgct cagcgtcggg caggagcagc tcctcctcct ccagcagccg
      721 caggatcggc tcgcccgcct cggcgatcag ccgcagctcg gacaggtcga aggtggagcg
      781 ggcgaagacg gatgccttgc ggaccacgtc gcgagcctcg cccggcaggt ggtcgatgcg
      841 ggcggcgatc acggcctgga ccgtgggcgg cagcagcgcc tggggtagcg gcccggtgtc
      901 cgaggggagc tcacctccca cgtggagcag catgccggtg gtctccacga tgaagaacgg
      961 gttgccgccg gcgtgccggg cgatgcgctc ggccgtgcga gggtcgagcc cctcccccgc
     1021 ctgcagggcg agctccgtgg catcgtccac cgacatcgac tccaggaaca gggtgagcga
     1081 gtcgcgcagg ccgccccccc agtccggacg ctggtcgagc aggtcgtccc gggccgcaca
     1141 gagcacgaac aggggcatgc ccctcgcgtc gcgaaccgtg tgctccacca ggtccaggag
     1201 atggggctcg gccaggtgca ggtcctcgaa gaccagcacc accggctccc gggccccgag
     1261 gccctccagg aaggccagca ggcccgagcg gacctcggcc acgcgcgagc gccggtcctc
     1321 ctgcgggtcg tccaccaggc tcaggacgtg accaaggcgg gccacgatct gctccgagcg
     1381 atcggccgag cagcaggaga gcacgacctc ctcgagccgg gcctgcagca ggtccttggg
     1441 cgcgtcgggg cgctctccca gctcctggag cagcatctgg cccacggggg cgaaggtgac
     1501 gtcttcctcg aaggggtcgg cgcgcccggt caggacgtgg acctgggcgg ggagcgcggc
     1561 cacgaactcg tcgatcaccc ggctcttacc gatgcccggc tcccccacga gggtcaccat
     1621 gtggccgcgg cgggactcca ccgctcgctc gaacgcgtcc cgcagcagcc ggagctcgcg
     1681 gcgacgatcc acgaagggga tggtccggcg cgtggagcgg ggcgccagcg ccaccaccgg
     1741 ccagccgggc agctgctcct cgaacccctt ggcctgaacc agccgccgct cgccgaactc
     1801 cacccgctcc agagcgatca tcctggtggt ctcgcccacc agcagtccgc cgggctcggc
     1861 cccctgctgg agcctggccg ccgcgttcac ggcggctccg aagagcagtc cctgctccgc
     1921 tgcctccgag ccgatcgcca ccgaccccgt gttgatcccc acccggcatc gcaggggaac
     1981 cggcagcccc agggactggc ccaggcgtcc cacccgatcg accacggcca gcccggcccg
     2041 gatcgctcgc agcgcgtcgt cgtcgtgcgc ctgcgggagc ccgaacacgc ccaccacggc
     2101 gtccccggcg aagttgtagg cgcggccgcg gagggactcg agctcctcgg tgacggcctg
     2161 gtagtaggcg gcgatcacct cccggtaggc ctccggatcg atccgggacg acagctgcgt
     2221 ggagcccacc aggtcggtga agacgaccgt gacgaccttg cgctcctcgg gtgggggcgc
     2281 ggtggtgggg aagccgcagt tcgggcagaa ccgggcgtcg acggggagcg gctccccaca
     2341 gcgctcgcac ctcgggcggt ccatccggca agcatacgtt ggggcgacga cgaccccgaa
     2401 cgctcccaga ccgctacggg accgacggct cgcccacgat ccggaagcct tcgatgtcga
     2461 ggatcgaccc cacccggcgc tccgcctcac ggaggatcat ctggacccgg gcccccacct
     2521 ccagcggctc atcacccggg tcgaggatcc ggtgcaccag gacggcgtcg gcgccgtccg
     2581 gacggatcag gccgatcgtc tcggggagat ccaacggccg ctcctcggga ccgacgaacc
     2641 cgatggtgaa ggacacgagc tctccgcccg gcccgacctc cgtgtcggcc gccagctccg
     2701 agaagcaccg ctcgcagaac agccgggccg ggacgtacgt gtagtcgcag ccctcacagc
     2761 gggagccgag caggaccccg cgatcgcgca gagccgtgaa gaaggcgtcc ccagccaccc
     2821 ccggcgtgta tcggtgaccg accgggaagt cgccctccca ccgacggacc aactcgggcc
     2881 gggcgatctt ctcgcgcatc agccctccgc caccggacgg aagtacgcga tgtcgaggat
     2941 cgacccggtg cgctcggcct gcggcttcca caccgcttcc acggccatgc cgatccggac
     3001 gtcgtccggc gccacctcgc cgagcttgtg gaggaagcca ccgctggaac cgtcgatcgc
     3061 gatcacggcg ggcagctccg gcggctcgat ccgctgcatg tcccaggaca cgtgacagac
     3121 cgagtacgtc tggaccacgc cgctcggctc cacctcggcc cagccatcgg tctcccggaa
     3181 gcaccgctcg cagaacatcc ggggtggcac cagcacccgg ccgcatccgt ggcacacccg
     3241 ccccaggatc cggccctcgg ccagcccgtc caggaacctg cccaccgcga cccccgcatc
     3301 ccacgcgtac cgagcctcga tcgcatcgtc caccgccccg gtcgccgacc ggaactcggc
     3361 atccgacagc ggatgtcccc ccatggcgaa cggcggggtc tgcacgaccg tgacgggttc
     3421 ggccccagga gccgccgggg gctctggagc tcgcgaagcg ggcggaacga gcgaagcgcc
     3481 ccccggcggg ccggcggccg aagccccatc gcggctcatg acgaccacgg tgccgacctg
     3541 catgagatcg ccccaggctt gggccacccc ccgttccggg gtgccgggga cctggcgggc
     3601 gccggcctcg ccgcggagct gccagaagag ctcggcgatc ttcatcaggc cggcggcggc
     3661 gatggggttg ccgacgccga gcaggccgcc gctggggcag ctcgggaggt ctccctcgcg
     3721 atcgagcaca ccgtcccgcg cggcctctgg ggccttgcct cgatcgaaca ggagcaggcc
     3781 ctcgaggtgg tggagctcct tgtagtcgaa ggggtcgtag ggctcggcca ggtggatctg
     3841 cttgcggggc tcggtgatgc cggccatgtc gtaggccatg cgggcggcgt tcgccacgta
     3901 ctcggggaac accaggtcgc ggttcgtcca gtaggcggtg tcgaggttcc accccacgcc
     3961 gtcgatccac accggggcat cggtgatgcg gcgggcggcc tcctccgatg ccagcaccag
     4021 cgccacggcg ccgtcggaga ccggcgagac atccagccgc tgcaccggcc aggccagggt
     4081 ctccgagccc agcacgtcct ccaccgtgat gtcggtgctt ccgagcagcg ccgccggatg
     4141 cccggccgcg ttgtgcttgt tcttcaccgc cacggccgcg atgtctcgct tgtccagccc
     4201 gtggaccgtc atgtagcggt tcatctccag ggcgaagatc cacagcaggt tgggtccgag
     4261 cggtcgctcc aggacgttgt cgaagatcgt gatgaacgcg ccctgcggat gcggctgggt
     4321 ggaggacatc ttctcctcgg ccaccagcag ggcgacgtcg aacagcccgc tggccaccga
     4381 gtaccagccc tggatcgcgg tgaacacgcc gctgccgcca cccacgtagg tgcggatgaa
     4441 gggcttgccg acggcgccgg ctccgtccga gaggtactcg cccttcatgt gcaccccatc
     4501 gaaggcgtcg ggggccgagg tcaggcagac gccgtcgacg tcggacagtg agagctcgca
     4561 ggagtccagc gccgcccggg ccgcctccca ggccagctcc ttgcccgtct cctgggcgcg
     4621 gcgcatgaag cgggtcatcc cggctccgac caccgccacc cggcgagcca tcagccgccc
     4681 ccctcgatga actcgatcag ctgcagcccg ttgccgaacg gatctcgaaa cgtcgcgtac
     4741 cgggcgtagc cgtcgacctc ccccgcgtgg tcgaacgtga cccctcgctc ctccaggcgg
     4801 gcccgagcgg tcaccagatc gtccacccgg aagaccgccg tgcctccggg cgccgacccg
     4861 gggccggccc cgtggagggc gagccggacg gctccggcgt cgaactcggc ccactcgtcc
     4921 ccgtcgcgac gcatcaggcc gagctcgagc acgtcacgat agaagaccac ggcccggtcc
     4981 atgtccccca cccagaacca gacgtggtcg agggtgttca cgctcacccg ccgaccccct
     5041 cgtcgttcgc cagcaccatc accgcggagg aggtggacgg cacgcccggc caggactggg
     5101 cgagggccgt gaagacctcg tccacctgcc gctcgccggc ctccccgcgc agctgctcca
     5161 cgcactcctg cagccgcacc agtccgttcg cctggaagag gttcccctgc ccgagcgccc
     5221 cacccgagac gttcacgggc aggtcgccgt cgggctcgag ctcgccgtcg gccagcagct
     5281 gcggcagctc ctcgccgtcg accaggccga gagcctccag gtgttcgagc tccttgtagg
     5341 cgtaggtgtc gtccagctcg gccaggtcga tctgctcgcg ggggtcctcg atgccggcca
     5401 tcgcgtacgc ccgctcggcg gcggctcgaa cggtatcggc ctccccccac cgccggctct
     5461 ccagggtggg gctccccgag caccacccgg tcccgaggat ccaggccggg gcctcggtca
     5521 ggtcccggac cagctcctcg gcggccagca ccacgaccac gcacccatcg gcgggtccgg
     5581 cgatctcgag ctcccggagc ggccagtacg agggtgccga atcgtccacc tcgtccgccc
     5641 ccaggtccga ggggtaggcg gcccgggggt tgtccagggc gttgcgccgg ttcttcgccg
     5701 ccacgatcgc gcagtgctct tcggacaggc cggttcgctc caggaaggcg gtcatgtcca
     5761 gaccggcgat cgccaggacc gacggcacca ccgggcggtt caggacggga tccagcgcga
     5821 accgctcgat ccggcccagc gtgagcacgt cggagacctt gctgtgcgcc tcgaccgcca
     5881 ccacccgggc ggcgccggcg ccgatcagca tgagggccgt ggccaccccg aacaggccgt
     5941 ccccggccac cgtctgcacc ggccggtgga gcgccccgag ctggtcgggc acgtactcgt
     6001 cgaagatgct ggtgccctcc agcaggtcct cggaacagca cacgaacgag tcgatgtccg
     6061 tccggggatc cacccccgcg tcgtcgtacg cgcgctgcgc ggcctcgaac atcagctcct
     6121 tgtaggacag cccggcggtg atgggagcga atcccgaatg cccgatcccg acgacggcga
     6181 cgcgcggctc cacgtctcct tctatcaccg aacgcgttcg agcgacgagc cgtggccctc
     6241 cggcgtcagc ggccggcgcc gaggatcctg cgcttctggg cctcgaactc ctcctgggtg
     6301 aggacccccc ggtcgcggag ggacgccaga cgctcgagct cgccggtcgt ggatggcgcg
     6361 ccgggtgcgg ggcctccccg catcatccgc tgctggttca tctcgccctg gtggtagatc
     6421 gtcttctgca cctcttcggg gttgcggatg tccgagaaga cgttggtccc acgctccccc
     6481 gccgactcga tgctgaggtc ccccgccccg atgatccgct cgaagacccc ctggctgaac
     6541 cgcacgtcgt tgatcgcctc gagcgggatc tccatcgagg acttggcgat ccacccctgc
     6601 cggtggatca cccggtcgct ggtgaccacg aagtgcgacg tggcccatcg cacggcccgc
     6661 cgcgccggat accagaccag gaggaggaac agggcgatca gcccacccca caccagcacg
     6721 gagcgaccga acccggagct cggcgcatag accaggagca cgatgtaggc ggccagcccg
     6781 aggatcgcga cggccgcggg gccgaccagg gcgatccagt gcggacgcag gtcgaggatc
     6841 agctcctctc cctccaccag cagccgcctc gggaacgcca tcggcgggac gctaccccgc
     6901 ccggggggcc gcggcaacca cggcgagcgc tcagcgcagg tgccggatct cgccgaagcg
     6961 gacgacctcc ggaccggcct gggtgctcac caccagacct ccggcctggt ccacatcgac
     7021 ggcccgcccc tcgatccgcg cccccgagac ggtggtggtg ctcacctcgt tgccgagcgt
     7081 cacgcagacg tcccggtacg aggaggtcac cgcgccggcg aaggccgggt gggtgggctc
     7141 gtaccggcgg acgaaggtcc gcaggaagtc ctcgagcagg tccgccggat ccaccgcctc
     7201 gatcgccgct gcgtggtgca cgtacggcgc ctggccgatg ttcacgccgg tccccaggac
     7261 cacgaaggcg aaccgatcct gctccaggag cgactcggcc aagatccctc ccaccttgcc
     7321 ctcccgggtc acgagatcat tcggccatcg gcatcccacc ggcgtgcccg agagccgaca
     7381 ggcggtggcc atggcccatc cggccagcag ggtgagcagc ccgccctgct ccgggtgcag
     7441 ctgggggcgg aggaccaccg agacgatcag cccccgatcg ggctcgtcca cccagctacg
     7501 gcccaggcgc cccctgccct ccgtctggtg gttggacccg accagggtcc actcgggcgc
     7561 cccttgccgc gccagctcca ccgccgtgag ctgggtggag cgggtaatct cgtcgaatcg
     7621 aacgggggcc tgcaccccga tccgctcgag cgcgtgcgtc agagcgtgtt cggatagcgg
     7681 catcggttga ctcccgttcg ggtgatgtgg attatcgcga gccggagccg cccgtgccga
     7741 agacccgcat attggtcgcg aaccgaggcg agatcgccgt ccggatcatc cggaccatcc
     7801 gcgagctcgg ctatcggtcc gtggccgtgt actcggatgc ggatcgggac gccctgcacg
     7861 tggagatggc ggactcggcg taccggatcg gtccgccgct gccggccgac tcgtacctgt
     7921 cgatcggcgc gatcctggag gcggctcgca aggcgaaggc caccgcggtg caccccggct
     7981 acgggttcct gtccgagcgc gcccatttcg ccaccgcggt cgaggagtcc gggttcctgt
     8041 tcatcggccc gccggccgag gccatcgaca tgatggggga caagtcggcc gcccggcagg
     8101 ccgccgatcg ggtcggtgtt ccgatcgtgc cgggcaccac ggagccggtc acggtgaagc
     8161 aagccaggcg ccacgcggaa cggatcggct tccccttgtt ggtgaaggcc gcgttcggcg
     8221 gcgggggcag ggggatgcac atcgtgcgga cgggcgcgga cctcgagccg gcgctgaccc
     8281 gggcggcgcg ggaggcgaag gcctacttcg gccggcccga ggtcttcctg gagcgctaca
     8341 tcgagcgcgc ccaccacgtg gaggcccaga tcctggccga tcgccacggc accatctcct
     8401 tcttgggcga gcgcgactgc tcggtccagc gccggcacca gaagctggtg gaggagacgc
     8461 cctccccggt ggtcgacgcc gagctgcggt cccggatcgg cgaggcggcg atgatgctgg
     8521 ccaaggaggc cgggtacacg aacgccggga ccgtcgaggg cgtcgtcgac gacgagggcc
     8581 acttctactt cctggagatg aacacccgcc tgcaggtgga gcacacggtg accgagatgg
     8641 tcaccgggct cgacatcgtg gccctgcaga tcgcggtggc cctcggcgag cacgtggacc
     8701 tgtcctcggt gcgcggacgc ggacacgcga tccagtgccg gatcaacgcc gaggacccct
     8761 cgaagaactt cctcccgggg cccggccggg tcgccgggta ccgcgagccg tccgggccct
     8821 tcgtgcgcgt ggactcctac ctgcgaccgg gcaaggaggt cccgtcggac tacgactcga
     8881 tgttcgcgaa ggtgatcgtg agcgcggagg accgggaacg ggcgcgccgt cggatgctgc
     8941 gggccctgga cgagttccag gtggagggca tccccaccac gatcccgctg caccggtggg
     9001 tgctcggcac ccgggagttc cgcgagggca cccacaccac cgcgtggctg gagcccgcac
     9061 tccgggacct gaagctgccg atgccggaga tcgccctgcc acccacggag gccaagcagc
     9121 cggtgccggc cgagctgctc gtcgagctgg acggccgaag ggtgcccgtc cggatcttcg
     9181 accagcggcg ggacaccgcc ccccggccgc cgagccggca cctgtccacc cacggcgagc
     9241 acgtccacag cgtggtggcc gcgcccatgc agggcacgat cttgcgcgtc ctggtggagc
     9301 agggccagga gatcgcggcc ggtgacgtgc tctgcatcgt cgaggccatg aagatggaga
     9361 acgcgatccc cgccccccgg gacggcatgg tttccgatct gccggtgcag gcggggcagg
     9421 tggtcgagcc cggacagacc ctcgccgtga tcgactgagc ccggcggaac cgctaccgct
     9481 cggggcgcgg cccccaggag tctcgtccgg tcatgcggaa cccggaatcg tggatccggt
     9541 agttgcggtt gaccgagacc aggaccgcgg tcagggtctc gacgatccgc gccatcgaca
     9601 ggtcgcccgc atccacccag cggaggtcgg ggatctggtc gatcacccca cccaccaggc
     9661 ccttggcctc acggtccctc ccgcagacga gcacgtcgct ctcgatggga tgctccaggt
     9721 cctgcagggc tccgccggcg atcgtgtgga aggccgccac catccggacc cccggatcca
     9781 ggatcgcctt ggcctgctcg gccgccgacc cgtgccaggg atgcaccacc tgccaggcgc
     9841 gaccacccac cgcggtagcc agcgggctgg tgcagtccac cacgatcttg ccggccgcca
     9901 cgtggtcctt gatggctcga tagatatccg cctggcccgc gtagggaacg gtgacgaaga
     9961 cgatctcccc ggcctcggcg gccgcctggt tcgtggtgcc gtccacccgg ccgtcggcgc
    10021 ccaggatccc ccgggccttc tccgcggtgg tggccccctt ctcctgtgat cgggatccga
    10081 tgatcgcgtg ctggccggcc cgggccaacc gcagcgtgag cccgaaccct tcctcgccgg
    10141 tgcctccgat gaccgccacg tccatggtgc gccagcctag cggaacggag cgaccccgcc
    10201 gggggggctt ccggcggggt cgctccagga ccaaggcccg ttggccctca cttcaccccg
    10261 gacctggccg ggacgaccgc caggtcgtac ccgtccgtcc cgttgcccca ggcatagacc
    10321 tggtaggtgt gcccgcgacg cagcttcagc acgtcggggc cgatcacggg ccggtagtca
    10381 ccgggcaggg agacccaggc cgcgtagacg cccctgggga ggacggccgt gaacgtctcg
    10441 ccgttgttca agccctcacc caggatggcg ccgttcgccc acaggtccac cgccggagcg
    10501 gccgcggtgt ggcggacgat cagccgggcc ctgccctcgc ccacctcgct catgtcgttg
    10561 cggaacaggc cgatggtggg ggcaccgttc gccttcaggt gagcgacggc cgtgtagttc
    10621 ctgccctcct tcagcctgat cccgtcggcc tccaggacgg ccggatcggc gcagtcgtcc
    10681 gaggcggcca cgaccttcag gtcgtacgag cccggaggca gcttgacgcc cttcacgacc
    10741 tcacccgggt tgaagtccga gatcgcctcg gccccgttca cacacacgtt cacgtccgcg
    10801 cccgggatgc cgtggacgac gttcagcgtc gccttcgctc cgccggcggc tcccgccggc
    10861 gctgcggcga gcagcgacat ggcgatcgac ccagcgaccg ccacaccgag cagcctcctc
    10921 gtgctgcgca tggctccctc ctcccctcgg ggggtgggcc gtctctccca cccctgcccg
    10981 agagcctggt ggaggagatg cactggcgtc acacatcccg tcggatctgc ataggttcgt
    11041 gcccgatgga cctctgcgag gagggcacag cctaggcact tgccttggat cggggaccgt
    11101 ccgcgtagag tccggtgcga tggcgcggac gatgacgtac ctgacggagc tcgtcgtggg
    11161 ggtggtctgc ctgggggccg ggaccggctt gatccgccgg gggccgagga tggccggcct
    11221 gctcctgggc gtggccgggg tggccgcggt ggttcacgcc atggtgcgct tgccgggctg
    11281 agcccgaggg ccgctaccgc ggccggtact cgccgaacgc ctcgcgcagc acgtcggaga
    11341 cctcccccag cgtggcccgg gcgcgcaggg cctccttcat cgggggcagg aggttggccg
    11401 atcctcgggc ggcggccccg accgccgcca gggatgcatc caccgcggcc cggtcccgac
    11461 cggcgcgcag ggcccggacc cgctcgacct gccgcgccac ctcctcctcg ccgatccgct
    11521 ggatttccac gggccgttcg tgcggatcga ggaagcggtt caccccgacc accacgcggt
    11581 tcccggcctc gatcccccgc tggatcttga acgccgcctc gtggatctcg tcctggtaga
    11641 agccctgctc gatggcggcc acggcgcccc ccatgccctc gatcttctcc aggtacctct
    11701 ccacccgttc ctgcagctgg tcggtgagcg tctccaggaa gtaggagccg gccagcgggt
    11761 ccaccgtgtc ggggacgccg gtctcgtacg ccagcacctg ctgggtccgc agggccagct
    11821 tcgccgcgtg ctccgtgggc agcgcgagcg cctcgtcgaa cgagttcgtg tgcagcgact
    11881 gggtcccacc caggacggcc gccagggcct cgaaggccgt ccgcaccacg ttggtctcgg
    11941 gctgctgcgc ggtgagcgtg gctccgccgg tctgcgtgtg gaaccgcagc atcgcgctcc
    12001 gagcatccac cgctccgaag cgttcccgca tgatccgagc ccacagacgc ctggcggtcc
    12061 ggaacttggc gatctcctcg aagaagttca tgtggcacgc gaagaagaag gacaggcggg
    12121 gcgcgaagtc atccacggcc aggccggcgg ccagggccgc ctgcacgtac gcgatcccgt
    12181 tggccagcgt gaacgcgacc tcctgcacgg cggtggcccc ggcctcgcgc atgtggtacc
    12241 cgctgatcga gatggtgttc caccggggaa tccggccccg gcagtacgcg aacaggtccg
    12301 tgatgatgcg catcgagggc ttgggggggt agatgtaggt cccccgggcc gcgtactcct
    12361 tcagcagatc gttctgcacg gtcccggaca gctggcccgg gtcgatcccc cgctcctcgg
    12421 cgaccagttc gtacagcagc aggagcacgg cggccgtcgc gttgatcgtc atcgaggtcg
    12481 agacgcgatc gagggggatc tgctcgaaca ggcgatgcat gtcctcgatc gagcagatcg
    12541 cgaccccggt cttgcccacc tcgccctcgg cccgcgggtg gtccgagtcg taccccatct
    12601 gggtcggcag gtcgaaggcc accgacagcc cggtctggcc ctgctccagc aggtaccgga
    12661 accgccggtt cgtctcctgg gccgtggcca tccccgcgta ctggcgcatg gtccagagcc
    12721 gaccccggta catcgtcggg tacacgcctc gcgtgtaggg aggctccccc ggcgccccga
    12781 ggtcccggcc cggatcgaag ccggcgaggt cgtcggggcc gtacacgggt cggacccgga
    12841 tgcccgagtc ggtgatgtgg cgttcccgct gccgggatgg ctcggccccg ctcgatcgat
    12901 cgcgttggtc catgagaccc agcgtagccc ctctcccccg ccggccgtcg cagccggggc
    12961 atccgtggac ggccgagtcg atcccgcacg gcctcggccg gagcatgccg ggtacgatgg
    13021 tggtcgtcgt gtcatccacc agcctcacct ccccgcacct cggagcctcc gcggccgacg
    13081 acctggtccg ccgagcgctg gccgaggacg caccgagcgg ggatcccacc acggcgctgg
    13141 tgatcccggc cggaacctcc tgctccgccg agctccgggc gaagcaggcc ggcatcgtgg
    13201 ccgggatgcc cgtggctcgg atggtcttcg agacggcggt cgagcaggat ggggccggat
    13261 cgatcggctt cacggctcgg gttcgggacg gcgccgaggt ccgaccggac gaggtgctgg
    13321 ccgtggtccg gggacccgcc cggaccatcc tgcgcgccga gcgggtcgcc atcaacctcc
    13381 tgtcccacct gtccggcgtg gccacgctca cccgagcctt cgtggacgcg gcggcgccgg
    13441 cgcggatcct gtgcacccgc aagaccaccc cggggctccg cgagctcgag cgcgcggccg
    13501 tggtggccgg cggcgggagc ctgcaccgag catccctctc ggatgcggtg ctgatcaagg
    13561 acaaccacgt ccgcatcgcc ggaggggttg ggacggccgt ccgccgggcc aggcgggagg
    13621 gtcatccggt cgaggtcgag gtcgagaccc tcgatcagct ggacgaggcc ctggccgagg
    13681 gcgcggagcg gatcctgctg gacaacgcca cccccgacct ggtgcggagg gcggtggacc
    13741 gggtgggaga cgcggggcgg ctggagatct ccggcggcgt gcggctggag acggtggggg
    13801 acctggtctc ggcgggggcg cgcgtgatct cggtgggacg gctcacgcac tcggccccgg
    13861 cgctcgacat ctcgttggag gtcatccgtg gggacgaaga cgcgcgcggc tgagggctcg
    13921 gaggcgctcg cggagcggtt cacgtcctgg gccgatgagg tccggcgact ggcgcaggag
    13981 cggcgcgccg tgatcctggc ccacaactac caggtcccct ggatccagga cgtggccgac
    14041 ttcgtgggag attcgctggc cctgtcccgt cgggccgcgg ccaccgacgc cgaggtgatc
    14101 gtcttctgcg gcgtgcactt catggcggag acggccaagg tgctgtcgcc ggacaagacg
    14161 gtgctgctgc ccgatctgga cgccggatgc tccctggccg acacggtcac ggccgagcag
    14221 gtgcggacct ggaaggccga gcacccgggc gccgccgtgg tggcctacgt gaacaccacg
    14281 gcggaggtga aggcggaggc ggacatctgc tgcacctcgt ccaacgcgag cgacgtggtg
    14341 ggctcgatcc ccgaggaccg ggagatcctg ttcctaccgg acatgttcct gggcgcgcac
    14401 gtggagcgcg tcaccggtcg gaagatgcac gtctggatgg gcgagtgcca cgtgcacgcg
    14461 gccatcgacc cggagcagct ggagcgcaag gcgttggagg accccgacgc cgccttcatg
    14521 atccatccgg agtgcggctg cacgacctcg gccctgtggc tgctgtccga gggcgtgctg
    14581 cccgccgagc gcacgcgggt gctctccacc ggcgggatgg tggccgaggc tcggcgctcc
    14641 tcgagcgacc ggttcctgat cgccacggag accggcatcc tgcatcagct ccggaaggcg
    14701 gcgccgcaga agatcttcga cccgatcgac gaggacgccg tctgccggta catgaaggcc
    14761 atcacccccg agaagctcct gcggtccctg cgggaggggg tcttcgagat cacggttccc
    14821 caggccgtcg ccgctcgggc ccggcgtgcc gtcgagcgca tgatcgaggt gggacccagc
    14881 tcggggcccg tctcccccgc tccctcgaac cgatcgcggt aggcgcggtg cgcaccgtgg
    14941 tcgtgggcgc gggcgcggcc ggcctgttct gcgccctgca cgccgccgaa cgaggacccg
    15001 tctcgctggt cgctccggcc gcagccctgg acgccgccac ggcgtgggcc cagggcggca
    15061 tcgccgcggc caccggcccg gaggactcgc ccgagctgca cgcggcggac acgatcgccg
    15121 cgggccgcgg tctgtgcgac ccggtcgccg tggccgtgct cgcgcgggag gcgccctggg
    15181 ccgtcgacga gctccgcgcc ctggggatgc ccttcgaccg tggggccacg gcgctggaag
    15241 ggggacaccg cgcccgccgg gtcctccacg cgggtgggga cgcgtccgga cgcgagctcc
    15301 accggttcct ggccgatcgc gtgcgagacg acgagcgggt ggagcgggtc cacggcctgg
    15361 tccgggcggt ggtccgggac ctggagggag cgcggggcgt gcggctggcc gatgggaccg
    15421 tgatcgaggg cgaccgggtg gtcctggcca ccggcggcgc ctgcgggatc ttcggtcacc
    15481 gcaccggacc cgagacctcc accggcgacg gcctggtgct ggccggggac gcgggagcgg
    15541 cgctggccga tctggaattc gtgcagttcc atcccacggc gctcgacgcg cccggccatc
    15601 ccgcccggct gctcaccgaa gcgctccgcg gcgagggggc gctcctcacc gatgccgagg
    15661 gccggagctt catgcaccgc ttccatccat ccggcgacct cgctccacgc gacgtggtgg
    15721 cccgcgccgt ggtccgcgtg cgcgaagaga ccggccgtcc ggtgttcctg gatgctcggt
    15781 ccgtcccgga cgtggcctcg aggttcccgg tcgccgccga gcaatgccga gaggtcgggc
    15841 tggacatcgc cgcccgcccg atccccgtcg cgcccgccgc ccactacttc atgggcggcg
    15901 tcctcaccga cacctggggt cggacctcgg tgcctcggct gttcgcctgc ggtgaggtgg
    15961 cggccaccgg ggtccacgga gccaaccgcc tggcgtcgaa ctcgctggcg gaggcgctgg
    16021 tcttcggcag gcgcgtggcc ctggccgagg acggctcgtg ggaggtgacc gaccgcggga
    16081 cacgggacgc cccccaacca ccggcccgcc ccggggggcc gggcctggcc gaactccggg
    16141 ccttgtgcga tgagcacctg ggggtccaac ggagcggtcc agacctcgac cggttcctgg
    16201 cggcgctgga ccccgcggac cggagccggg acggatcggc cgcacgggtg gcccggctga
    16261 tggccctcgc ggcgcgccgc cgcaccgaga gccggggcgg gcacttccgg gtcgacttcc
    16321 ccggcatcga cccggcgtgg cgggtccggc aggccgtgga tcgcgatggc tggtgggcgc
    16381 tgccggtccc ggatccgcgg ccttcggacg gggtcccccg ggaagaacgg cccgggtcga
    16441 gctccgtcgt tccgcagtag actcccgccc ggtcgcacgg cgcatcggcc cgaacggcgg
    16501 agctccacac gctggggtga ggaacgagga cgatggcgaa acgcaagacc gatgtcggca
    16561 cgctgtacaa gggcagtccg ggacagtggt cctggctggc ccatcgcatc accgggatcg
    16621 ccgtgatcct gttcctgttc gcccacgtgg tcgacacggc ggtggtcggc tggggccccg
    16681 gagcctatga ccgcgtgatc caggtctacg agaactgggc cgtgcggatc ctcgagctcg
    16741 gactggtggc ggcggtcatg taccacgcga tcaacggcgt gaagatcatg atcttcgact
    16801 tctggcccag ctccacccgc tacttcaaga cgatctccct gatcagcacc ggcatcttcc
    16861 tggcgggcat ggtcccgatc acctggatca tgggcaagga catcatccac ctggccttcg
    16921 gaggggcgtg atggccaccc gcaccgagag ccgaccggcg ggcgagcggc ccgtcccgcg
    16981 cagcggctcg atgctcgggt accg
//
LOCUS       DASQQQ010000119         2519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_36201, whole genome shotgun sequence.
ACCESSION   DASQQQ010000119 DASQQQ010000000
VERSION     DASQQQ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36201"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VE646_05075"
     CDS             complement(<1..203)
                     /locus_tag="VE646_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019069999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79393.1"
                     /translation="MAVVTGASRGIGRAVAERLAAQAPASPSSPAPSTRWPRPRGRCR
                     GMAYGSSEEVVAMWPKSYAEQIR"
     gene            complement(345..1955)
                     /locus_tag="VE646_05080"
     CDS             complement(345..1955)
                     /locus_tag="VE646_05080"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006939923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZD79394.1"
                     /translation="MVTSLDEPTVASSDHGFLPIDDLELRSRIDGMLNRHPAVGLAVG
                     VVRNGSLEFYGHGTADIASNTPITADTVFRIGSITKTFTAIAVMQLQERGLIDLDAPA
                     NDYLRAYELVPAQAGFRPATVRHLLTHTAGIPEARHVCDLFHPEAGPFGGRPLHLSVR
                     FGERLPSLAEYYRDGLRVVAQPGTAFAYSNHGYATLGQIVEDVTGTPLERYFRERIFE
                     PLGMEDTDLLRSERVASRLATGYGLGRRGAEAVPDRDWIGAAGGGIYSTTRDIARFAA
                     ALLGGGANQHGSVLEPATLATMFEAYHRPDPRLPGWGLGFARAEAGTHRVVGHDGILP
                     GFNSELLVAPDDGVGVVAFTNGSKGAFMWMETEFKRLLRHLLDAPDEVVRTDIAHHPE
                     VWEELCGRYRLPARISDLRQRLAIAAGVEVFVRGGRLMLRALAPIPALYRGLPLHPDD
                     EDDPYVFRLDLSGLGMSAVRVVFGRDVASDTAAIHTSLGGQPLSLIRRPTEGRARGPL
                     AAALGGLLVATATRSVTRRRQRSKEAAA"
BASE COUNT          386 a          922 c          878 g          333 t
ORIGIN      
        1 ctccggatct gttccgcgta gctcttcggc cacatggcca ccacctcctc cgagctccca
       61 tacgccatcc cgcggcagcg cccccgcggc ctcggccacc tggtgctggg agcgggcgag
      121 gagggcgacg ccggcgcctg ggcggccagt cgttccgcga cggcccggcc gatcccgcgg
      181 ctcgcgccgg tcaccaccgc cacctgcccc ttcaggaccg gcccaaccgc ggtctcctgg
      241 gaggtgggcc tccacgacct ccacgctgga cgccggcctc gtcctgatcg cccgggccag
      301 ccccgtggcg ctcatcctcc aaagctcggc ggccgttgcg aggctcatgc cgctgcctcc
      361 ttggatcgct gacgacgccg cgtgacggat cgcgtggcgg tggccaccag cagtccgccc
      421 agcgcagcag cgagcggccc acgcgccctc ccttcggtgg gtcgcctgat gagcgacaac
      481 ggctgtcccc cgagactggt gtggatggct gccgtgtcgc tggcaacgtc gcggccgaac
      541 acgacgcgga cggccgacat ccccaacccc gagaggtcga gccggaacac gtacgggtcg
      601 tcctcgtcgt cggggtgcag cggaagaccc cggtagagcg cggggatggg agcaagcgcc
      661 cggagcatca ggcgtccacc gcgcacgaac acctccacgc cggcggcgat cgccaggcgc
      721 tggcggaggt cggagatccg cgcaggcagc cgatagcgac cgcacagctc ctcccagacc
      781 tcggggtgat gagcgatgtc agtgcgcacg acctcgtcgg gtgcgtcgag caggtggcgg
      841 agcagccgct tgaactcggt ctccatccac atgaacgccc ccttcgagcc gttcgtgaag
      901 gcgacgacgc cgacaccgtc gtcgggggcc accaggagct cggagttgaa gcccggtagg
      961 atcccgtcgt ggccgaccac gcggtgcgtg ccggcctcag cgcgggcgaa cccaaggccc
     1021 caccccggca ggcgaggatc cggccggtgg taggcctcga acatcgtcgc cagcgtcgcg
     1081 ggctccagca ccgacccgtg ctggttggct ccaccaccca gcagcgccgc ggcgaagcga
     1141 gcgatgtcgc gggtggtgga gtagatcccg ccgcctgcgg cgccgatcca gtcccgatcg
     1201 gggaccgcct ccgcgccgcg gcggccgagg ccgtagcccg tcgccagacg cgacgcgacc
     1261 cgctcggagc ggagcaggtc ggtgtcctcc atcccgaggg gctcgaagat ccgctcacgg
     1321 aagtagcgct ccaggggcgt cccggtcacg tcctcgacga tctggccgag cgtggcgtac
     1381 ccgtgattcg agtacgcgaa ggcggtgcct ggctgggcga ccacccggag accgtcgcgg
     1441 tagtactcgg ccaacgacgg cagccgttcc ccgaacctca cgctgaggtg aagaggccgt
     1501 cccccgaagg gccccgcctc cgggtggaac aggtcgcaga cgtggcgcgc ctcggggatc
     1561 ccggcggtgt gggtcaggag gtggcgcacc gtcgcgggcc ggaagccggc ctgcgccggg
     1621 accagttcgt aggcgcgcag atagtcgttc gccggcgcgt cgaggtcgat gagacctcgc
     1681 tcctgcagct gcatcacggc gatcgcggtg aaggtcttgg tgatggaccc aatccgaaag
     1741 accgtgtccg cggtgatcgg cgtgttcgag gcgatgtccg cggtgccgtg tccgtagaac
     1801 tcgagggatc cgttgcggac cacgcccacc gccagaccga cggcgggatg gcggttcagc
     1861 atcccgtcga tcctcgacct caactcgagg tcgtcaatcg ggaggaaccc atgatccgaa
     1921 gacgcgaccg tcggctcgtc gagtgacgtc accatcgtct ccaccctcct cgctcccccg
     1981 ggcaccccga accagcctcc gcgcccggcg gccactccac gctcccaccc gaggacagcc
     2041 ggtacaggag cccttgagcc cgctccgagg gggaccttcg ggcgccctga cggccgggcg
     2101 agccccggga gtggcgtccc atcggccggg gcaatcagcg ttcccccgcc ccaggacctc
     2161 agaagcccgc tggagttggc cacgatgggc gaagggcgtc ctcgccggac tgctggagga
     2221 agcgagcgcg gctgaagagc cggatcacgg agcacccggg cgcgagggtg gacaagcccg
     2281 ctatcgcctt ccctccgcgg ctcaccccgg gcaagcccgc cggccgatcc aacggtgcag
     2341 ccccacagta aagggcagta ctttggagca tggcaaccat cgacgatgcg gcccggctgg
     2401 ccctggggct cccccaggtc accgaaggcg agcggcacgg caaccgcacc tggtcggtcg
     2461 gcgggaaggc cttcgcgtgg gtgcgaccgt tcagcaaggc ggacctcagg cggttcggc
//
LOCUS       DASQQQ010000120         5541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_392033, whole genome shotgun sequence.
ACCESSION   DASQQQ010000120 DASQQQ010000000
VERSION     DASQQQ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392033"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..904
                     /gene="cpaB"
                     /locus_tag="VE646_05085"
     CDS             236..904
                     /gene="cpaB"
                     /locus_tag="VE646_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HZD79395.1"
                     /translation="MPWPAGAPAFLLVRGYAARLEPLRPVLGNPVDVVVAARALERGT
                     TLAADDLAVRRIPSVYAPPGVLHAGPEAVGGVLVAGLAPGEPLTRTRLAGTHAGPVAA
                     LVPTGLRAFAVASGIASGMVRPGDRVDVLATFGGQRPYTETVAEGLEVARVMGTEPGP
                     GSGPSPSTATVGQAATVVLLVDPAQAERLAHAAALGTIAIAVAPPSDLPPTDAAPTQT
                     SVPR"
     gene            973..1863
                     /locus_tag="VE646_05090"
     CDS             973..1863
                     /locus_tag="VE646_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HZD79396.1"
                     /translation="MLGITQGITEFAPVSSSGHLILVPWFFGWSILKDPILNKTFDVA
                     LHVGTLIGALLYFRNDVVRYLAAWIRSIRGRRMATVDERLAWVLVIGTIPGAVAGAAF
                     SNLIETRLGQPWLIASMLVVFGVVLVAVDRMVAARSSRSVAMAGDGATVMVDGVARGR
                     TIEDVGIRDGVIVGVAQAVALQPGVSRSGVTITAARAVGLEREPAARFSFLLSLPIIA
                     GAGVFKGAELVREGMPPGMAGPFLWGMISAAVSGFLVIWFLLSYLRRHDFMIFLWYRV
                     AVAAIVFAVILAGVRPASGL"
     gene            complement(1826..2962)
                     /locus_tag="VE646_05095"
     CDS             complement(1826..2962)
                     /locus_tag="VE646_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZD79397.1"
                     /translation="MVRPRRVLVTGGAGFIGSHVVDQLVSEGIEVRVLDSLPPGAHGR
                     EPDYLNGGAEYVVGDLRDGEMVERALEGVDAVSHQASMVGLGTDLEDAPDYVANNDLG
                     TAVLLRAMHRVGSVDRLVLASSMVVYGEGAYRCVRHGSVHAPPRDPRRLAAGAFEPTC
                     PGCDRSLVPEPVSEVAAVDPRNVYAATKLHQEHLGAVFAREHGAGFVALRYHNVYGPR
                     MPRLTPYAGVASIFLSALAEGQAPRVLEDGRQRRDFIHVRDVARANVLALTVEAVPNG
                     PLNVATGRPRTVGEMAAALTSAFGPGAPVPEVTGGFRLGDVRHVFASTDRAEQRLGFR
                     AGVPFEVGMAELARAPMRAAPTPLIATGAMRAPTGRRPDAPRRG"
     gene            complement(2956..4293)
                     /locus_tag="VE646_05100"
     CDS             complement(2956..4293)
                     /locus_tag="VE646_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79398.1"
                     /translation="MSVRTRDPVRDSSIPAVGVRRSGLRIRVAIVVSGAVLLVEASWW
                     WGRGLQGDGTRLFLNAPPLTGHLDPRFPARALGAVAVAGIIVWLGPLLAQRAGWRRLL
                     LVAVAGAALWAAALAAADGVAGFLIGPSSRLGYLHDVPLVGSPGAFLSGFVDRIGMYS
                     AHVRAHPPGMVLLLWSLDRLGLSGPGWVAALEILGGAAAVPAASIAVREVAGEAAARR
                     AVPFLVLAPAAVTLAASGDALFTGVAAWAIALVVLATGRTGRTSDGLAWVGGVLFGAT
                     LFLSYGLVLLSLLPLAVAIGRRRFRPLAAAAVGIAAVVGAFGAAGFWWGAGLLAARRE
                     YLASVARFRPYGYFLVADLAAFAVILGPAAIAGLVRLRHRATWLLVGGALVAVALADV
                     SGMSKGEVERIWLPFAPWVLLAAAALPERTGRAWLAATAVAALALQLLVVSPW"
     gene            complement(4290..4772)
                     /locus_tag="VE646_05105"
     CDS             complement(4290..4772)
                     /locus_tag="VE646_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79399.1"
                     /translation="MSTSARARAGRGRSFWIGLVLGWAAIAFGILSLLSHAGATHPIV
                     FGLYVVGSALAHDLVLVPLTLAFMALVAPRLPVRLRGPVTGASVVSAVVVLFAIPALA
                     GFGRLPDNPSLLPRNYVGGLAAVLAAVWVVAGLVVLARLAGNRRARGAGTVPPSRAAR
                     "
     gene            complement(4769..>5541)
                     /locus_tag="VE646_05110"
     CDS             complement(4769..>5541)
                     /locus_tag="VE646_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZD79400.1"
                     /translation="WPFSFPPAHYAAAWITIGALIVHVGAKLPVAARALPARSPERRA
                     ERDPRRGADGQAAGLSRRGFLAATAASAGALTVATAGGTLRPLAAVSVLAPRDPRSGP
                     QGVPVRKTAGSAGVEAAARDPGYRLTVEGNVANPLSLSLEDLGRLPQHRTSLPIACVD
                     GWSAQAEWRGVRVRDLLAMAGAPPQAEAEVVSLQRPGSAYATSDLNRYHAADPDTILA
                     LEVNGESLSLDHGFPLRLVGPNRPGVMQTKWVTRVVVR"
BASE COUNT          795 a         2102 c         1983 g          661 t
ORIGIN      
        1 cgcgatgatc gtgggccggg agcagctgga ggagttccgc actcgggtca tgcccctggt
       61 ccagcgcgaa ccccgacgcc ggtagcacct cggggcgccg ggcgccgtga accacgtcac
      121 agccagcgcg gctcgcctcc actaccctcg ccggcgctgg tatcggatgc cgagcgcagg
      181 aggcgccccg tgatgctccg ccgttggtcc ccggccgcga aggctctgat gctccttgcc
      241 ctggccagcg ggggcgcccg cgttcctgct cgtccggggg tacgcggcgc gactggagcc
      301 gctccggccg gtgctgggca acccggtcga cgtcgtggtg gcggcccggg ccctggagcg
      361 agggaccacg ctggcggccg atgatctggc cgtccggcgc atcccgtcgg tctacgcgcc
      421 gccgggtgtc ctccacgccg gtcccgaggc cgtgggtggc gtgctggtgg ccgggctcgc
      481 ccccggcgag ccgctcaccc gcacgcgcct ggccggcacg cacgccggac cggtggcggc
      541 cctggtgccc acgggccttc gagccttcgc cgtcgcgtcg gggatcgctt cgggcatggt
      601 gcgaccggga gaccgggtcg acgtgctggc gacgttcggg ggtcaacgcc cgtacaccga
      661 gacggtggcc gagggcctgg aggtggcccg ggtgatgggc accgaaccgg gtccgggatc
      721 ggggccgagc ccgtccacgg cgaccgtcgg gcaggccgcc acggtggtcc tgctggtcga
      781 cccggcgcaa gccgagcgac tggcccacgc cgcggccttg ggcacgatcg ccatcgccgt
      841 ggctccgccg tcggatctgc cacctacgga cgccgctccg acccagacat cggttccccg
      901 gtagcatgtc cgccacgtgc ctacggcggg ccaccgggaa ggagccgagc gacgagcatc
      961 ctgcaagcga tcgtgctggg gatcacccag gggatcaccg agttcgcccc ggtctccagc
     1021 tcgggacacc tgatcctggt gccctggttc ttcggttgga gcatcctgaa ggacccgatc
     1081 ctcaacaaga ccttcgacgt cgccctgcac gtcggcacgc tgatcggtgc cctcctctac
     1141 ttccggaacg acgtggtccg gtacctggcg gcgtggatcc gttccatccg gggcaggcgg
     1201 atggccaccg tggatgagcg gctggcgtgg gtgctggtga tcgggacgat ccccggggcg
     1261 gtggcgggcg ccgctttcag caacctgatc gagacgcggc tcggacagcc ctggctgatc
     1321 gccagcatgc tcgtggtctt cggcgtggtg ctggtggcgg tggaccggat ggtggcggcg
     1381 cgatcttcgc gctcggtggc gatggcgggc gacggcgcca cggtgatggt ggatggagtc
     1441 gcgaggggac gaaccatcga ggacgtcggg atccgggacg gcgtgatcgt gggggtggcg
     1501 caagcggtcg cgctgcagcc cggggtgtcc cggtcgggcg tgaccatcac ggcggcccgg
     1561 gccgtggggc tcgagcgcga acccgccgcc aggttctcct tcctactgtc gctgcccatc
     1621 atcgccggcg ccggcgtgtt caagggagcg gagctggtaa gggaagggat gccgcccggg
     1681 atggcgggcc cgttcctgtg gggcatgatc tcggccgccg tcagcggctt cctggtgatc
     1741 tggttcctgc tctcctacct gcgacggcac gacttcatga tcttcctgtg gtaccgggtg
     1801 gccgtggcgg cgatcgtgtt cgccgtcatc ctcgccgggg tgcgtccggc ctccggcctg
     1861 taggggcgcg catcgcgcct gtcgcaatca gcggcgttgg tgcggcccgc atcggtgccc
     1921 gcgccagctc ggccatcccc acctcgaacg gcacccccgc gcggaacccg agccgctgct
     1981 ccgcccggtc ggtggaggcg aacacgtgcc ggacgtcgcc cagccggaac ccgccggtca
     2041 cctccggaac cggtgctccc gggccgaacg ccgaggtcag cgccgcagcc atctcgccca
     2101 ccgtgcgggg gcggccggtg gccacgttga gggggccgtt cggtacggcc tccaccgtca
     2161 gcgccaggac gttggcccgc gccacgtcgc ggacgtggat gaaatcgcgt cgctggcggc
     2221 cgtcctcgag cacccgcggg gcctgcccct cggccagcgc gctcaggaag atgctggcca
     2281 cccccgcgta gggcgtcagc cggggcatcc ggggtccgta cacgttgtgg taccggaggg
     2341 ctacgaagcc ggcgccgtgc tcgcgcgcga acaccgcgcc gaggtgctcc tgatgcagct
     2401 tggtggccgc gtagacgttg cgcggatcca ccgcagcgac ctcggacacg ggctcgggga
     2461 ccagcgaccg gtcgcatccc gggcaggtgg gctcgaaggc cccggcggcc aggcgacggg
     2521 ggtccctggg tggggcgtgg accgatccgt gccggacgca ccggtaagcc ccctccccgt
     2581 acacgaccat gctgctggcc agcaccaggc ggtccacgga acccacccga tgcatcgccc
     2641 gcagcaggac cgccgtcccc agatcgttgt tcgccacgta gtcgggcgcg tcctccagat
     2701 cggtcccgag tcccaccatc gaggcctggt ggctgaccgc atcgaccccc tccagcgctc
     2761 gctccaccat ctctccatcc cggaggtcgc ccaccacgta ctcggcaccc ccgttcagat
     2821 agtccggttc ccgtccgtgc gccccgggag gcagggagtc cagcacccgg acctcgatgc
     2881 cctcgctgac cagctggtcc accacgtggg agccgatgaa tccggctccc ccggtcacga
     2941 ggacccggcg aggcctcacc acggcgagac caccaacagc tgcagggcca gcgccgcgac
     3001 cgcggtggct gccagccagg cacgtccggt ccgctccggc agggccgccg cggccaggag
     3061 cacccacggg gcgaacggca gccagatccg ctccacctcg cccttggaca tcccggacac
     3121 atcggccagc gccacggcca ccagcgcgcc gcccaccagc agccaggtgg cccggtgtcg
     3181 caggcgcacc agccccgcga tggcggccgg tcccaggatc acggcgaacg cggccaggtc
     3241 ggccaccagg aagtagccgt agggccggaa ccgagcgacg ctggccagat actcgcggcg
     3301 ggcggccagc aagccggccc cccaccagaa gcccgccgcg ccgaaggcgc cgaccaccgc
     3361 ggcgatcccg accgccgccg cggccagggg gcggaaccga cgacggccga tcgccacggc
     3421 cagcgggagg aggctcagga ggaccagccc gtaggagagg aacagggtcg cgccgaacag
     3481 caccccgccg acccacgcga gaccatccga ggtccggccc gtccgtccgg tggccagcac
     3541 gaccagggcg atcgcccacg cggccacccc cgtgaagagc gcgtcgccgg aagccgccaa
     3601 cgtcacggcg gccggcgcca gcacgaggaa gggcacggcc cgtcgcgcgg ccgcctcccc
     3661 cgccacctct cgcaccgcga tcgaggcggc ggggacggcg gcggcgccgc ccaggatctc
     3721 cagggcggcc acccaccccg gacccgacag ccccaggcga tcgagcgacc agagcagcag
     3781 gaccatcccc ggcgggtgcg cccgcacatg ggccgagtac atcccgatgc gatccacgaa
     3841 cccggacagg aacgccccgg gagacccgac cagcgggacg tcgtgcaggt accccagccg
     3901 gctggagggg ccgatgagga acccggcgac cccatcggcg gcagccaggg cggccgccca
     3961 cagcgccgca ccggccacgg ccaccagcag cagccgccgc caacccgccc gctgggccag
     4021 cagggggccc agccacacga tgatcccggc gaccgcgacc gcccccagcg cgcgagcggg
     4081 gaagcgtgga tcgagatgac cggtgagcgg gggcgcgttc aggaacaggc gtgtgccgtc
     4141 cccctggagc ccgcgacccc accaccagga ggcttcgacc agaagcaccg cgcccgacac
     4201 gacgatcgcg acccggatcc ggagcccgga ccggcggacc cctaccgcgg ggatcgagga
     4261 gtcccggacc gggtcccggg tgcggacgct catcgggcag cccgcgacgg cgggacggtc
     4321 cccgctcctc gtgcccgccg attcccggcc agccgggcca gcaccacgag cccggcgacc
     4381 acccacacgg ccgccaggac tgccgccagt cccccaacgt agttgcgggg cagcaggctc
     4441 gggttgtcgg gcagccgccc gaaccccgcc agggccggga tcgcgaagag cacgaccacg
     4501 gcgctgacca ccgatgctcc ggtgaccggc ccgcgcaacc ggaccggcag ccggggcgcc
     4561 accagggcca tgaacgcaag ggtcaggggc accagcacca ggtcgtgcgc cagggccgag
     4621 cccaccacgt acagcccgaa gacgatcggg tgggtcgccc cggcgtgcga gagcaagctc
     4681 aggatgccga aggcgatcgc cgcccacccc aacaccagcc cgatccagaa ggaacgaccg
     4741 cgtcccgcgc gcgcccgcgc gctcgtactc atctcaccac caccctggtc acccacttgg
     4801 tctgcatcac gcccggtcgg ttcggtccga ccaggcgcag cgggaagccg tgatccaggc
     4861 tcaggctctc gccgttgacc tccagagcca ggatcgtgtc ggggtcggcc gcgtgatacc
     4921 ggttcaggtc ggaggtcgcg taggccgagc cgggccgctg gagcgagacc acctccgcct
     4981 ctgcctgggg cggcgctccg gccatcgcca acaggtctcg cacccggacg cctcgccact
     5041 cggcctgcgc gctccaccca tccacgcagg cgatcggcag cgacgtacgg tgctggggga
     5101 ggcgcccgag gtcttccagc gagagggaga gggggttggc gacgttgccc tccaccgtca
     5161 ggcggtaccc ggggtccctc gccgccgcct ccacgccggc gctgccggcg gtcttcctca
     5221 ccggcacccc ttgcggtccc gaacgtggat cgcgcggggc cagcaccgac accgcggcca
     5281 gcggtcgcag ggtcccaccc gccgtggcca cggtcaacgc gccggccgac gccgcggtcg
     5341 ccgccaggaa cccgcggcgc gagagccctg cggcctgtcc gtccgcacct cgtcggggat
     5401 cccgctctgc ccggcgctcc gggctgcgcg cgggcaaggc gcgggccgct acgggcagct
     5461 tggcgcccac atggacgatg agggcaccga tcgtgatcca tgccgcggcg tagtgcgcgg
     5521 gcgggaacga gaagggccag g
//
LOCUS       DASQQQ010000121        11052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_392042, whole genome shotgun sequence.
ACCESSION   DASQQQ010000121 DASQQQ010000000
VERSION     DASQQQ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11052
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392042"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VE646_05115"
     CDS             complement(<1..371)
                     /locus_tag="VE646_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZD79401.1"
                     /translation="MQVAWAGGRRVVVITLLLVAAFLAQGAVVQSAHASTRSRDTMLR
                     LTNHDRSEHHEASLSLNAKLSRYALRHSRRMAEKGYLFHTIDLADRLKGLDWSIGGEN
                     VGVGSTLHRVERGFMRSTPHR"
     gene            complement(511..1326)
                     /locus_tag="VE646_05120"
     CDS             complement(511..1326)
                     /locus_tag="VE646_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018252029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZD79402.1"
                     /translation="MRVALCQMNSGDDVDANRRSADELLEGAAAGGADLASLPEVWTY
                     LGSARRHREVAEPVPGPTSEWLGSVAAEHGMWVLGGSLLELGPDRRVFNTSLLFDRSG
                     RQVAAYRKIHLFDVRLPGQPPIRESASYTAGEQVVTAETQFGRFGLSICYDLRFPELY
                     RSLALQGAQIVFVPSAFTEETGRDHWDVLLRARAIEDQVFVIAAAQWGTWGPPEQHRR
                     CFGNSLVVDPWGRVLARAPDGIGVTFADLDLDEVGRVRERLPALQHRRLPPSC"
     gene            1350..1955
                     /locus_tag="VE646_05125"
     CDS             1350..1955
                     /locus_tag="VE646_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79403.1"
                     /translation="MAVGRYTVMDGSGNPVGTEEFRCAPGPMGWRYFSDVETSVPEPH
                     HEVIDLTVDADWRPVRTRIQTGSHEIELSASTDRLSGFLDGLPVRFPWGLEHHLDYLS
                     PAFNAATTNRLSATGDIDVVYLDPVTCEPRSVRQRYELLGSDEVATPVGRFAAVRWRY
                     TAMSSGWSRDLWVAADVVVRYEDVFELAWYEPGASGPLPRS"
     gene            complement(1956..2537)
                     /locus_tag="VE646_05130"
     CDS             complement(1956..2537)
                     /locus_tag="VE646_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZD79404.1"
                     /translation="MAQAEKPQVELPAPGVWELDPQHTAVEWVARHLLTKVRGRFRTF
                     TGAIEVAERPEESRVEVEIDTASIDSGTPDRDNHLRSADFLEVETYPKLTFRSSTLRP
                     TGTNSFDLEGELTIRDVTRPVTLQAEYLGVHDSPWGTKVAAFSAATEIDREEFGLTWN
                     VAIETGGWLVSRDVRIELEIEAVYQAEAAREAS"
     gene            2653..3009
                     /locus_tag="VE646_05135"
     CDS             2653..3009
                     /locus_tag="VE646_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZD79405.1"
                     /translation="MPPDGLSPYCHHFHRAVQMIARRWTPEILRALLAGTVRFSGFTA
                     VIPGLSDRLLSERLKDLEAEGIVVRTVTPATPVRVEYRLTAKGEDLATAIGAIAAWAE
                     RWVTEREAVESEAGSH"
     gene            3115..4422
                     /locus_tag="VE646_05140"
     CDS             3115..4422
                     /locus_tag="VE646_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZD79406.1"
                     /translation="MSDDAAQLLERHRKVLPSWMLLYYEQPIELVRGEGTRVWDGAGG
                     EYLDFFGGIVTTISGHLMERMVHELTEQIESGINHTSTLYLIRPQIELAEKLVELAPI
                     DHPAKAFFVSSGTEAIEAALLFATSWRRSNEIIALRNSYHGRSFGAVGITANKGWAPT
                     SFSPLKVSHAIEPYCYRCPLGLSYPTCGVACAEDLRNVIVTTTTGEPAAMIAEPIQGV
                     GGFVTPPQEYFQIVKGILDEFGIPFIADEVQTGFGRTGEAFWGIGSYGVQPDAIVVAK
                     GLGNGLAIGAVVGRTDMVESLRANSISTFGGNPISTRGALANLDNVLERDLQQNAYKM
                     GSYLMRGLKDLEDRYDVVGEVRGKGLMIGVEMVTDGTSKEPDAQGANRVMESARERGV
                     LIGRGGLYGNVLRLSPPLTITEDDAERAVETLDVAFGELQATR"
     gene            complement(4419..5684)
                     /locus_tag="VE646_05145"
     CDS             complement(4419..5684)
                     /locus_tag="VE646_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZD79407.1"
                     /translation="MRATDPWWTHPGLQVRDGRLLVAGRDAQGLSRDHGTPLYVYDLA
                     RIGEQAAALQGAFARRGVPFRLRLALKAQRDPAVLGYLRSLARPGSARGVGLDVCSPG
                     ELAHGLRHGWRPEEISYTGTNVSERDLDAILAHPVHVNVDLLSQLERFGRRGRGRRVG
                     LRVNPRRGAASGVGRADLYSGGARPSKFGILEEQLPEALAVAGRLDLTIDTVHFHVGD
                     GFLDEGLPRFAKAVDAVARMTGTLLEAGCPIAEVNAGGGLGVPQGPGQAPLDLDAYAG
                     ILVERLGPLGVGLAAEPGDFLTKEMGVLLAEVVSMELREGVTFVGLDAGYNVAPERFI
                     YGSPLPVVPCLGPDAPPAGPVTITGNINEGDDVFAEDLPLPAVAEGDVLAMLGVGSYN
                     ASMHLDHCLRPAARVVAFPHRRGRRPELG"
     gene            complement(5694..6452)
                     /locus_tag="VE646_05150"
     CDS             complement(5694..6452)
                     /locus_tag="VE646_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZD79408.1"
                     /translation="MELSVVERWFAAREVRDGLTLLWEPHVDPYLISNIWHLRGSERD
                     LLVDTGNGLGRLRPVIEAAARGRPVIAVATHAHFDHVGGLTEFDDRWIHRADAPALRG
                     AEDPLRLRRSDLSPAFLADMAFYGFAPPEVLVHALPRPDFDVAGFRTPACQPTRLLED
                     GDRVELGDRSFVVLHVPGHTPGSLALWDGSSGVLFTGDTVYADDPVSGADRDAFHRSL
                     RRLRDLPVSLVCAGHNRPIGPEELVRIIDGLLRG"
     gene            6565..6843
                     /locus_tag="VE646_05155"
     CDS             6565..6843
                     /locus_tag="VE646_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79409.1"
                     /translation="MPDDETLPVGALEEEKQDPATSPSRPQPSEAEVESARLLANDAR
                     PTLQKEGMSYDTIRRLADEFIARDLGEGLPEFLDWARARTQPEERPSD"
     gene            6912..8306
                     /locus_tag="VE646_05160"
     CDS             6912..8306
                     /locus_tag="VE646_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZD79410.1"
                     /translation="MAALLAFALVLLVAVLLSGLAHRSVLSTALVFLVAGFISGDAVT
                     GVVKVNPGQSNLELFIDLALFSVLFTDGMRVGIRQLAGAWRLPGRALLLGMPLTLGII
                     AGLAHLMFDLPWIEALLMGAALSPTDPVLAAAIVGREEVPARLRHLLNVESGLNDGLA
                     LPVVIATLAVASENRVPLPQLLLQIGGGVAIGVVVPWLAIRLGRLRIFGVAGLYEPLG
                     AFAIGTIVFALAGLLGYNMFLAAFAAGVTIATLSTAVRDAFRRFGELVTELLKLGALF
                     LFGALIPISAVGGVGWLLAVGFALLVLLVARPAALAVSLLGSQLDVSERVTAAWFGPK
                     GFASVAYGLIILHGGISHGSLLFDVIALVTAISIVAHSSTDVLVARWFRRLREEGTGG
                     EASGDAVGQARAEAEAEREVGEQPEIEHPAERGEEPEPAEAEETAEQEAARARGDQAG
                     SEQPPDRAGSGEQR"
     gene            8350..9180
                     /locus_tag="VE646_05165"
     CDS             8350..9180
                     /locus_tag="VE646_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HZD79411.1"
                     /translation="MSTGRDDEETGSDEPSDELRIAFEATVDEGVRRLTRSWPRLVAT
                     GLVGGIDISVGVFALLLTLQETHSVPVGALAFGIGFLALLLARSELFTENFLVPIAAV
                     VATKARWRDILRLWAGTAVTNYLGGWIVMAVALTAYPGIRNEAVSLAVYYPRLGIGWT
                     SFASALMAGTVMTLMTWMERGTTSTTAKMVASFSAAYVLATGHLDHAIISGLEMFAGL
                     QAGAPFGYVDWARTVGWAALGNMIGGIGFVTVLRIVQVGGRQLSEERNRPLSEPRGGG
                     "
     gene            complement(9206..>11052)
                     /locus_tag="VE646_05170"
     CDS             complement(9206..>11052)
                     /locus_tag="VE646_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZD79412.1"
                     /translation="WEDSAVAPEKVGPYLRDLRELYRTYGLRGALYGHLGDGCIHSRI
                     SFDLRTASGLKNYRAFMEEAADLVVSYGGSLSGEHGDGQQRAELLPKMFGEDLVQAFR
                     EFKAIWDPDWKMNPGKVVDAYRLDENLKLGADYQPWQPDVRFAYQEDGGDFAHAALRC
                     VGVGKCRIPGGVDVMCPSFMVTREEKHTTRGRARLLFEMLQGDVVTDGWRSDEVYEAL
                     DLCLACKGCTNDCPVNVDMPTYKAEFLHHHWKGRLRPRQAYAFGLIDRAAQVASWMPG
                     LANLVSSVPPSARLLKLAGNVAPSREVPCFAPLTLQDWFARRGDRNPGGPPVILWPDT
                     FNNYFHTDVGVAAVEALEAAGWHVIMPRRHVCCGRPLYDYGFLDLAERYLRRSIDEVR
                     QHIRQGVQVVGMEPSCLATFKDELPKMLPHDEDGKRLRLQSFHFPDFFREHDIPLPKL
                     NRRALVWGHCHHKATGGIDPEVQLLRTMGLDVETVTGGCCGLAGSWGFEHGHEEVSMA
                     AGEQALLPAVRRAPQDTIVVADGFSCKTQIEQGGTGRRALHVAQVLQLARELGPEGPA
                     EGKPEDRFTGARPSASAGRKLKRAAGLMGAGVAAGIGVAAGLAAARTR"
BASE COUNT         1616 a         3946 c         3867 g         1623 t
ORIGIN      
        1 tcccggtgcg gcgtgctgcg catgaaccct cgctcaaccc gatgcagggt gctcccgacc
       61 cccacgttct caccgccgat ggaccagtcc aggcccttca gcctgtcggc caggtcgatg
      121 gtgtggaaca ggtacccctt ctcggccatg cgacggctgt gtcgcagggc gtatcgagag
      181 agcttggcgt tcaggctgag cgacgcctcg tggtgttcgg accgatcgtg gttcgtgagc
      241 cggagcatgg tgtcccgcga acgggtgctc gcatgcgcgc tctggaccac ggccccctgg
      301 gccaggaagg cggccacgag cagcagcgtg atgaccacca ccctgcgacc cccggcccac
      361 gcgacctgca tcgccacctc ccgccgtccg gcggccagcc gctcagacgc accggccgtc
      421 cctgcgcagg aggtatcggc tgaacgcgct gtgcggttga gccgaacgac cgatccggac
      481 ccccctcgaa cccggagggg ggatcgagcc tcagcaggaa ggcggcagcc ggcgatgctg
      541 cagcgccggg agtcgctcgc ggacccggcc cacctcgtcc agatcgagat cggcgaaggt
      601 caccccgatc ccgtccggcg ctcgagccaa cacacggccc cagggatcga cgaccaggct
      661 gttgccgaag cagcggcgat gctgctcggg cggcccccag gtcccccact gggccgcggc
      721 gatcacgaag acctggtcct cgatggcccg agcacgaagc aggacgtccc agtggtccct
      781 gccggtctcc tccgtgaacg cggaggggac gaagacgatc tgggcccctt gcagggcgag
      841 ggagcggtac agctccggga atcgcaggtc gtagcagatc gacagtccga accgcccgaa
      901 ctgcgtctcg gccgtgacca cctgctcacc cgccgtgtag ctggccgact cgcggatcgg
      961 cggctgtccc ggcagccgga cgtcgaacag gtgaatcttg cgataggcgg ccacctgtcg
     1021 gcccgagcgg tcgaacagca ggctcgtgtt gaacacccgg cgatccggtc ccagttccag
     1081 gaggctgcct cccagcaccc acatgccgtg ctcggcagcg accgacccca accactcgga
     1141 ggtcgggccc ggcaccggct cggccacctc ccgatggcgc ctggctgagc cgaggtaggt
     1201 ccagacctcg gggagggagg cgaggtccgc gccgccggcg gccgccccct cgagcagctc
     1261 atccgccgag cgccggttgg cgtcgacgtc gtcgcccgag ttcatctggc acagagcgac
     1321 cctcacttcg gcatcctacg atggggcccg tggcggtcgg ccggtacacc gtgatggacg
     1381 gatcggggaa ccccgtcggc accgaggagt tccggtgcgc cccagggccg atgggatggc
     1441 ggtacttctc ggacgtggag acctcggtgc ccgaaccgca ccacgaggtg atcgacctca
     1501 ccgtggatgc cgactggcgc ccggtgcgca cccggatcca gaccggctca cacgagatcg
     1561 agctctcggc gagcacagac cggttgagcg gtttcctgga cggcctgccg gtgcggttcc
     1621 cttggggcct cgagcatcac ctggactacc tctctccggc cttcaacgcg gcgaccacga
     1681 accgcctgtc ggccaccggg gacatcgatg tcgtgtacct ggacccggtg acctgcgagc
     1741 cgaggtccgt ccggcagcgc tacgagctgc tggggagcga cgaggtcgcg acccccgtcg
     1801 gacgcttcgc cgccgtgcgc tggcgctata cggcgatgtc gtcgggctgg agccgcgacc
     1861 tctgggtggc cgccgacgtg gtggtccggt acgaggacgt cttcgagctg gcgtggtacg
     1921 aaccgggcgc gagcggcccg ctgccgcggt cctagctacg aggcctccct cgccgcctcg
     1981 gcctggtaca cggcctcgat ctcgagctcg atccgcacgt cccggctcac cagccaacct
     2041 ccggtctcga tcgcgacgtt ccaggtgagc ccgaactcct cccgatcgat ctcggtggcg
     2101 gcggagaagg cggccacctt ggtcccccat ggggagtcgt ggacccccag gtactcggcc
     2161 tgcagggtga ccggacgggt gacgtccctg atcgtcagct cgccctccag atcgaacgag
     2221 ttggtgccgg tgggccgtag ggtcgagctc cggaaggtga gcttcgggta cgtctcgacc
     2281 tcgagaaagt ccgcgctccg cagatggttg tcccggtcgg gggtcccgct gtcgatgctc
     2341 gcggtgtcga tctccacctc cacccgggac tcctcgggcc tctcggccac ctcgatcgcg
     2401 ccggtgaacg tgcggaaccg gccgcggacc ttcgtcagca ggtgccgcgc cacccactcc
     2461 accgcggtgt gctgcggatc cagctcccag acgccggggg cgggcagttc cacctggggc
     2521 ttctcggcct gcgccatcgt cctcctcctt cgctatgctt acccaacact ggggactaac
     2581 ttgtcataag gaagcgttat tccgtacatg agcacctttc ccccgccgcg tggggcacct
     2641 accatgccgg acatgccgcc cgatggcctg tccccctact gccaccattt ccaccgggcg
     2701 gtgcagatga tcgcgcgccg ctggacgccg gagatcctcc gggccctgct ggcggggacg
     2761 gtgcggttca gcgggttcac ggccgtgatc cccgggttgt cggaccgact cctgtcggag
     2821 cgcctgaagg acctggaggc cgagggcatc gtggtgcgga ccgtgacgcc cgccaccccc
     2881 gtccgggtgg agtaccgcct gacggccaag ggcgaggacc tggccaccgc gatcggcgcg
     2941 atcgccgcct gggccgagcg atgggtgacc gaacgcgagg ccgtggagtc cgaggcgggc
     3001 tcgcactagc cacccccccc atcgtcgggg tcagcaccaa ggagagccgc tcggcctccc
     3061 gggggtgcct tcccggcgat atcgtgactc ccacagccac acgagggggt cgcgatgtcc
     3121 gacgatgctg cccagctgct ggagcgacac cggaaggtcc tgccgtcctg gatgctcctg
     3181 tactacgagc agcccatcga gctggtccgg ggcgagggca cgcgcgtctg ggacggagcg
     3241 ggtggcgagt acctcgactt cttcgggggg atcgtcacca cgatctcggg ccacctgatg
     3301 gagcggatgg tccacgagct caccgagcag atcgagagcg ggatcaacca cacctcgacc
     3361 ctgtacctga tccgtccgca gatcgagctg gccgagaagc tggtggagct cgcgccgatc
     3421 gaccatccgg ccaaggcgtt cttcgtgagc tccggcacgg aggccatcga ggcggcgctc
     3481 ctgttcgcga cgtcctggcg gcgctcgaac gagatcatcg ccctccgcaa ctcgtaccac
     3541 ggccggtcct tcggggccgt cggcatcacc gccaacaagg gatgggcacc gacctcgttc
     3601 tcgccgctga aggtctccca cgcgatcgag ccgtactgct accgctgccc gctcgggctg
     3661 tcgtacccga cctgcggcgt ggcctgcgct gaggacctcc gcaacgtgat cgtcacgacc
     3721 accaccggcg agccggcggc catgatcgcc gagccgatcc agggggtggg cggcttcgtg
     3781 accccgcccc aggagtactt ccagatcgtg aagggcatcc tcgacgagtt cggcatcccg
     3841 ttcatcgccg atgaggtcca gaccggcttc ggccggaccg gcgaggcgtt ctgggggatc
     3901 gggtcctacg gggtgcagcc cgatgccatc gtcgtggcca agggcctggg caacgggctc
     3961 gcgatcggcg cggtcgtcgg ccggaccgac atggtcgagt cgctccgcgc gaactcgatc
     4021 tcgaccttcg gtggcaatcc gatctccacc cggggggccc tggccaacct ggacaacgtg
     4081 ctggagcggg acctgcagca gaacgcctac aagatgggct cgtacctgat gcgtggcctc
     4141 aaggacctcg aggaccgcta cgacgtggtc ggcgaggttc gcggcaaggg gctgatgatc
     4201 ggcgtcgaga tggtgaccga cgggacgtcc aaggagcccg acgcccaggg ggcgaaccgg
     4261 gtgatggagt cggcccgtga gcggggggtc ctgatcggcc gcggcggcct gtacggcaac
     4321 gtgcttcggc tctccccgcc gctcaccatc accgaggacg acgccgaacg cgcggtggag
     4381 acgctggacg tcgccttcgg ggagctgcag gccacccgct agccgagttc gggccgccta
     4441 ccccggcggt gcgggaaggc caccacccgg gccgcggggc gcaggcagtg gtccaggtgc
     4501 atcgaggcgt tgtagctgcc caccccgagc atggccagca cgtccccctc ggccaccgcg
     4561 gggaggggca ggtcctcggc gaacacgtca tcgccctcgt tgatgttgcc ggtgatggtg
     4621 accggaccgg cgggtggagc gtcgggcccg aggcagggca ccacgggcaa gggcgatccg
     4681 tagatgaagc gctcgggggc gacgttgtag ccggcatcca ggcccacgaa ggtgaccccc
     4741 tcccggagct ccatcgatac gacctcggcc agcagcacgc ccatctcctt cgtgaggaag
     4801 tctcccggct ccgctgcaag tcccacccca agggggccga gacgctccac caggatcccc
     4861 gcgtaggcgt ccagatccag gggggcctga ccgggtccct gcggcacccc cagcccacct
     4921 cccgcgttca cctcggcgat cgggcagccg gcctcgagca gcgtgccggt catccgggcc
     4981 accgcatcta cggccttcgc gaaccggggc agcccctcgt ccaggaaccc gtcgcccacg
     5041 tggaagtgga cggtgtcgat cgtgagatcg agcctgccgg ccacggccag cgcctctggg
     5101 agctgttcct ccaggatgcc gaacttcgag gggcgcgctc ctccgctgta caggtccgcg
     5161 cggccgaccc ccgaggccgc gccccgtcgg gggttcaccc gcaggcccac gcggcgtccc
     5221 cgtccccgcc gaccgaaccg ctcgagctgc gagagcaggt ccacgttgac gtggacgggg
     5281 tgggccagga tcgcgtccag gtcacgctcg gagacgttcg tccccgtgta gctgatctcc
     5341 tccggccgcc acccatgtcg gaggccgtgc gccagctcgc ccggggagca cacatccagg
     5401 cccacgcccc gcgccgaacc gggccgggcg agcgatcgca ggtagcccag gaccgcggga
     5461 tcgcgctgag ccttcagcgc gagccgcagc cggaacggga ccccccggcg ggcgaacgcc
     5521 ccctgcaggg cggcggcctg ttcaccgatc cgggccaggt cgtagacgta cagtggggtg
     5581 ccgtgatccc gagacaaccc ctgcgcatcc cggccggcca ccagcaggcg gccgtctcgc
     5641 acctggagcc cgggatgcgt ccaccacgga tcggtcgcgc gcatcggcag aggctacccg
     5701 cgcaggagtc cgtcgatgat ccggacgagc tcctcggggc cgatcggccg gttgtggccg
     5761 gcgcagacca gcgacacggg gaggtccctc aggcgacgga ggctccggtg gaacgcgtcc
     5821 cgatcggcgc ccgacaccgg gtcgtccgcg tagacggtgt cgccggtgaa caggaccccg
     5881 ctcgacccgt cccacagcgc gaggctgccg ggggtatgac cgggcacgtg cagcaccacg
     5941 aacgaccgat cgcccagctc cacccggtcg ccgtcctcca gcagccgggt gggctggcag
     6001 gcgggcgtcc ggaaccccgc gacgtcgaag tcgggtctgg gcagggcgtg gaccagcacc
     6061 tcgggtggcg cgaagccgta gaacgccatg tccgccagga aggccggcga cagatcgctc
     6121 cgccgcagcc ggaggggatc ctcggccccc ctcagggcgg gagcgtcggc gcggtggatc
     6181 cagcggtcgt cgaactcggt caggcctccc acgtggtcga agtgcgcgtg ggtggcgaca
     6241 gcgatcacgg ggcgcccgcg ggcggccgcc tcgatcacgg ggcggagccg ccccagtccg
     6301 ttgcccgtgt ccaccagcag gtcccgctcg ctcccccgca ggtgccagat gttcgagatc
     6361 aggtatgggt cgacgtgcgg ctcccacaag agcgtgagcc cgtcccgcac ctcgcgcgcg
     6421 gcgaaccagc gctcgaccac cgacagctcc atgagcctcc cgcgacggat atccgcccca
     6481 tcctgccaga cgcggtcgac gatgacgttt gccccgctct caccacggga accagcgcag
     6541 tgacgttccg cggcaggagg accgatgccc gacgacgaga cgctcccggt gggagccctg
     6601 gaagaggaga agcaggaccc ggccacgagc ccctcccggc cccagcccag cgaggccgag
     6661 gtggagagcg cccgcctgct ggccaacgac gcgagaccca ccctccagaa ggaggggatg
     6721 agctacgaca cgatccgccg cctggcggac gagttcatcg cccgggacct gggggaggga
     6781 ttgccggagt tcctcgattg ggccagggcc cggacgcagc ccgaggagcg cccctccgac
     6841 tgatcgccgc gcccgctcgg cgctcggccc cggcggtttg tggcgcgcga ggcccagggt
     6901 aagtccgggt cgtggccgcc ctcctcgcgt tcgccctggt cctgctggtg gccgtcctgc
     6961 tttccggact ggcgcaccgc agcgtgctgt cgacggcact ggtcttccta gtcgcggggt
     7021 tcatcagcgg cgacgccgtc actggtgtgg tcaaggtgaa cccgggccag tccaacctcg
     7081 agctgttcat cgacctcgcg ctgttcagcg tgctgttcac ggacggcatg cgggtgggca
     7141 tccggcagct ggccggcgcg tggcggctgc cgggccgagc gctgctgctg ggcatgccgc
     7201 tcaccctcgg gatcatcgcg ggcctggccc acctcatgtt cgacctgccc tggatcgagg
     7261 cgctcctgat gggcgccgcg ctgagcccca cggaccccgt gctggcggcg gcgatcgtgg
     7321 gccgcgagga ggtcccggct cggctgcggc acctactcaa cgtcgagagc gggctgaacg
     7381 acggcctcgc gctgcccgtg gtgatcgcca ccctggccgt ggcctccgag aaccgggtgc
     7441 ccttgcctca actgctcctc cagatcggcg gcggggtcgc catcggggtc gtggtgccct
     7501 ggctggccat ccgcctggga cgccttcgga tcttcggggt ggccggcctg tacgagcctc
     7561 tcggggcgtt cgcgatcggc acgatcgtgt tcgccctggc cgggttgctg gggtacaaca
     7621 tgttcctggc cgcgttcgcc gccggagtga ccatcgccac cctgtccacg gccgtccgcg
     7681 acgccttccg ccggttcggc gagctggtca cggagctgct caagctggga gccctcttct
     7741 tgttcggggc gctgatcccc atcagcgcgg tgggtggcgt gggatggctg ctggccgtcg
     7801 gcttcgcgct gctggtcctg ctggtggcca ggccggcggc gctggcggtc tcgctgctcg
     7861 ggagccagct ggacgtgagc gagcgcgtga ccgcggcgtg gttcggtccc aaggggttcg
     7921 cctccgtcgc gtacggcctg atcatcctcc acggcggcat ctcccacggc tccctgctgt
     7981 tcgacgtgat cgcgctggtc accgcgatct cgatcgtggc gcactcctcc accgacgtgc
     8041 tcgtcgcacg ctggttccgg cggctgcggg aggagggtac cggcggggag gcatccggcg
     8101 acgcggtggg gcaagctcga gcagaggcgg aggcggagcg ggaggtcggg gagcagccgg
     8161 agatcgagca ccccgcggaa cgcggcgagg agcccgagcc tgcggaggcc gaggagaccg
     8221 cggagcagga ggcggcgcgg gcgaggggcg accaggccgg gtcggagcaa ccgcccgacc
     8281 gagccggttc gggtgagcag cgatgacgcc gggcagcgtg gaagccatgg acctgggtac
     8341 gtagccgccg tgagcacggg gcgggacgat gaagagacag gctcggacga gccctcggac
     8401 gagctgcgga tcgcgttcga ggccacggtg gatgagggcg tccggcgcct cacccgttcc
     8461 tggccccggc tcgtcgcgac ggggctggtg ggcgggatcg acatcagcgt cggcgtcttc
     8521 gccctgctcc tcaccctgca ggagacccac agcgtgccgg tgggagcgct ggccttcggg
     8581 atcgggttcc tcgcgctcct gctggcgcgg agcgagctct tcacggagaa cttcctggtg
     8641 ccgatcgccg ccgtcgtggc caccaaggcg cggtggcgcg acatcttacg gctgtgggca
     8701 ggcacggcgg tcacgaacta cctgggcggg tggatcgtga tggccgtcgc cctgaccgcg
     8761 tacccgggga tccgcaacga ggcggtgtcg ctggccgtgt actacccgcg gctggggatc
     8821 ggctggacct ccttcgcctc cgcgctgatg gccgggaccg tcatgaccct gatgacctgg
     8881 atggaacgag ggaccacctc caccacggcg aagatggtgg cctccttctc ggccgcgtac
     8941 gtcctggcca ccggacacct ggaccacgcg atcatcagcg gcctggagat gttcgccggc
     9001 ctccaggccg gagccccctt cggctacgtc gactgggcca ggaccgtggg gtgggccgcg
     9061 ctcgggaaca tgatcggcgg catcggcttc gtaacggtgc tccgcatcgt ccaggtgggg
     9121 gggcggcaac tgagcgagga gcggaaccgc cccctcagcg agccgcgggg cgggggctga
     9181 tacccccgga gccagagacg aagggtcagc gggttctggc ggcggcgagg ccggccgcca
     9241 cgccgatccc ggccgcgacc ccggctccca tcagcccggc cgcccgcttc agcttgcggc
     9301 ccgcggaggc cgagggccgg gccccggtga agcgatcctc cggcttgccc tcggccgggc
     9361 cttcgggtcc cagctcccgt gccaactgca ggacttgggc gacgtgcagg gcccgtcgtc
     9421 ccgtcccccc ctgctcgatc tgggtcttgc aggagaagcc gtcggcgacc acgatcgtgt
     9481 cctgcggcgc ccggcgcacg gccggcagca acgcctgctc acctgcggcc atggacacct
     9541 cctcgtgccc gtgctcgaac ccccacgatc cggccagacc gcagcagcca ccggtgacgg
     9601 tctccacgtc caggcccatg gttcgcagca gctggacctc gggatcgatc ccgccggtcg
     9661 ccttgtggtg gcagtgcccc cacaccaggg ccctgcggtt cagcttcggg agcgggatgt
     9721 cgtgctcgcg gaagaagtcg gggaagtgga acgactgcaa gcgcaagcgc ttgccgtcct
     9781 cgtcgtgcgg cagcatcttc ggcagctcgt ccttgaaggt ggccaggcag ctgggctcca
     9841 tccccaccac ctgcacgccc tgccggatgt gctggcggac ctcgtcgatc gagcggcgca
     9901 ggtaccgctc ggccaggtcc aggaacccgt aatcgtacag gggtcggccg caacagacgt
     9961 ggcggcgggg catgatgacg tgccaccccg ccgcctccaa cgcctccacc gcggcgacac
    10021 cgacgtccgt gtggaagtag ttgttgaacg tgtccggcca caggatcacg ggggggccgc
    10081 cggggttgcg gtcgccccgc cgcgcgaacc agtcctggag cgtgagcgga gcgaagcacg
    10141 ggacctctcg gctcggagcc acgtttcccg ccagcttcag gagccgcgcc gaggggggca
    10201 cggacgagac caggttggcc agccccggca tccacgaggc cacctgggcg gcgcgatcga
    10261 tcagcccgaa cgcataggcc tggcggggcc gcagccggcc cttccagtgg tggtgcagga
    10321 actcggcctt gtaggtgggc atgtccacgt tcaccggaca gtcgttcgtg caccccttgc
    10381 acgccaggca caggtccaga gcctcgtaga cctcgtcgct ccgccaccca tcggtgacga
    10441 cgtccccctg gagcatctcg aacagcagcc gggcccggcc acgcgtggtg tgcttctcct
    10501 ccctggtcac catgaagctt gggcacatca cgtcgacgcc cccggggatg cggcacttcc
    10561 caacccccac acagcggagc gccgcgtggg cgaagtcacc gccgtcctcc tggtaggcaa
    10621 accggacgtc cggctgccag ggctggtagt cggccccgag cttcaggttc tcgtccaacc
    10681 ggtaggcgtc gaccaccttg ccggggttca tcttccagtc cgggtcccag atcgccttga
    10741 actcgcggaa ggcctggacc aggtcctcac cgaacatctt gggcagcagc tcggcccgct
    10801 gctggccgtc gccgtgctcg cccgagagcg atcccccgta cgacaccacc agatcggcgg
    10861 cctcctccat gaaggcgcga tagttcttca gcccggaggc ggtccgcagg tcgaacgaga
    10921 tcctggagtg gatgcatccg tcgcccaggt gcccgtacaa cgcgcctcgg aggccgtacg
    10981 tgcggtagag ctcgcggagg tctcgaaggt agggaccgac cttctcgggc gccaccgccg
    11041 agtcctccca cc
//
LOCUS       DASQQQ010000122        11274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_392090, whole genome shotgun sequence.
ACCESSION   DASQQQ010000122 DASQQQ010000000
VERSION     DASQQQ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11274
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392090"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VE646_05175"
     CDS             complement(<1..351)
                     /locus_tag="VE646_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase catalytic subunit"
                     /protein_id="HZD79413.1"
                     /translation="MSRSLAVDLRGLRLPVPVLAASGCSGTGRELSGLVDHRALGAVV
                     TGSLTYGPSRGAPTPRVAESRAGIVTAVGQNPGVRDFMAEDLPRLRRLRIPVIASVGG
                     SSLEEYVRVTSALQG"
     gene            complement(348..1220)
                     /locus_tag="VE646_05180"
     CDS             complement(348..1220)
                     /locus_tag="VE646_05180"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HZD79414.1"
                     /translation="MRRARAEILNVRRQGGFHSMTLVAPQIAEKARPGQFISVAMPPD
                     RGFILRRHFSIHQASRRGGWAGTLEFVFDPRGPGTAWLAEMKAHRFVDVIGPLGKSFA
                     YPQRLTNCLLVSEGHGTAQIYFLAQELRARGKRVDMLIGGTEESRVFRPIEGKRLSQT
                     VTVVTRDGSLGSQGTVAEALPAVTASTGAQVVYAAGPRSVLRAVAGFCAERRLPAQVA
                     VEAEMACGLGLCYTCVVPVIRKDGSGYDNLRACTEGPVFNPARIFWDRWMNDEPRTVP
                     TPPEGFPAVTAWPG"
     gene            complement(1217..4495)
                     /gene="carB"
                     /locus_tag="VE646_05185"
     CDS             complement(1217..4495)
                     /gene="carB"
                     /locus_tag="VE646_05185"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HZD79415.1"
                     /translation="MPRRTDMDRILVIGSGPIVIGQACEFDYSGTQACKVLRGEGFQV
                     SLVNSNPATIMTDPEFSDRTYIEPLTPESVLSVIRREKPDAILPTLGGQTGLNVAVAL
                     AESGELDRLGVSLIGASLDAIHRAEDRSGFQRAMTEAGLPVPRAGIAHGVDEALAVAD
                     AIGYPVIVRPSFMLGGGGSGFADGPAGLRELAARSLAESPAHEVLVEESVAGWKEFEL
                     EVMRDGADNAVIVCSIENVDPMGVHTGDSITVAPQQTLTDREYQEMRDAALACIRAIG
                     VETGGSNIQFAVDPRDGRQVLIEMNPRVSRSSALASKATGFPIAKIAALLAVGYRLDE
                     ITNDITGQTPAAFEPTLDYVVVKIPRFAFEKFSEADPTLSTTMKSVGEVMAIGRTFKE
                     ALGKAWRSMERDGFDLGWLGLRGILHDEPPPSAPAHSLLDLLRIPSEGRLHLVELALR
                     EGLSVEGIARRSGIDAWFVDQIAQVVEEASALQGRALGSLGAGEVLEAKRVGISDRRI
                     GRLTGATEGEVRARRSELGVEVVYKTVDTCGGEFPATTPYHYSTYEEESEVRPAERPR
                     VLILGAGPNRIGQGIEFDYACVHAAYALREAGYEAVMVNSNPETVSTDYDTSSRLYFE
                     PLSAEDVLAVCRVERPVGVIVQLGGQTPLKLAPTLQEHGFEVLGTAPEAIDLAEDRGK
                     FATLCAELGIPAPPHGEARDLEEAREVARRIGYPVVVRPSYVLGGRAMEIVYGEDELE
                     RFVRSAAEASPDHPVLIDRFLEGAIEIDVDAVADGTDVFVGAVMEHIEEAGVHSGDSS
                     CAIPPPTLSDDELDEVERITRALAERLGVRGLMNLQLAVKDERIWVLEANPRASRTVP
                     FVSKATGVPLAKVATLVLLGRSLREQTALGLVPADPAHYRRLPYTCVKAAVLPFGRFP
                     GVDTVLGPEMRSTGEVMGIDQDSGSALAKALVSSGATLPAAGTVFVSVANRDKRAIAF
                     PARRLSELGFRLLATRGTAGVLERAGIAVERVDKVSEGSPNVADMIAGGEVDLVINTP
                     FGRSPRTDGYYIRTAAARAGIPCITTLPGVLAALRGIEALREDPAEPLAIQDYHELAG
                     RSPG"
     gene            complement(4495..5628)
                     /gene="carA"
                     /locus_tag="VE646_05190"
     CDS             complement(4495..5628)
                     /gene="carA"
                     /locus_tag="VE646_05190"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HZD79416.1"
                     /translation="MRVGSKGSRAARQALLVLEDGSRFRGRAFGAGGETFGEAVFNTA
                     MSGYQEVLTDPSYAGQVVVMTAPHQGNYGTNGLDPESGRVRVAGFAVREASRRASSWR
                     GERTLAQELGAAGVVGIEGIDTRRLTLRLRSEGAMRCGLSTEDLDPHSLVERVRATQG
                     MEGADLAAGVSAEEPFEARSVVGPAATDLGRVFRVAAYDFGMKRTILRLLAAAGCETT
                     VFPARTPALEVLGDGFDGVFLSNGPGDPAATRYGVEATRALLGRLPVFGICLGHQLLG
                     LALGTRTFKMKFGHRGANQPVKDLATERVEVSSHNHGFAVDPASLGDRVAMTHVNLND
                     GTLEGLRALDVPAFGVQYHPEAAPGPHDARHLFGRFRELMGAH"
     gene            complement(5630..6931)
                     /locus_tag="VE646_05195"
     CDS             complement(5630..6931)
                     /locus_tag="VE646_05195"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014693424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZD79417.1"
                     /translation="MPDRILFRGARLVDPASATDAVRHVLVERGRIAAIGEDASPPAG
                     TEVLDCTGLVLAPGLVDPHTHLREPGREDRETVETGSRAAVAGGYTAITAMANTDPVA
                     DTAAVIEEVRSLALRAGLCDVSPVGAITKGLEGAEMAAIGEMSAIGVRMFSDDGRCVP
                     TSRVLRNALEYAKAFDVVLAEHCEDAALSEDTQMHEGVHSYTLGLAGEPAEAEEVIVY
                     RDLALARLTGGRLHLLHLSTAGSVELVRQAKAEGLRVTAEATPHHLTFTHEDLLNYDT
                     NRKVKPPLRTAEDRAALRAALADGTIDVVGTDHAPHAVEEKEAEFDHAPPGTIGLETA
                     LAAILTHLVEPGILTLERAIEVLSTAPARLLGLSEHGGPVEPGGPAHLVAFDPQAEWV
                     VEPPFQSRSRNSAFLGRRLRGRVIHTVHRGELVVCDGKPQR"
     gene            complement(6924..7874)
                     /locus_tag="VE646_05200"
     CDS             complement(6924..7874)
                     /locus_tag="VE646_05200"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HZD79418.1"
                     /translation="MNKHLLSINDVSAQDIVRILDTAESFREVGTRVIKKVPALRGRT
                     VVNLFYENSTRTRISFELAAKRLSADVINFTTSGTSVAKGESLKDTALTLQAMGADAV
                     VIRHSSSGSPFTLTRWIRGSVLNAGDGTHEHPTQALLDLYTIRERLGRFEGLRTAIMG
                     DVLHSRVARSLSFALVKMGAEVTLVGPPTLIPPDAPAWGVQVSYDIDAVLPKLDVCYM
                     LRVQRERQRQQYFPSVREYARLFGLTRERVGVLPEGALIMHPGPMNRGVEIDSDVADL
                     PQAVMEEQVTNGIAVRMSLLYLLLGGSGSSSDEGEGAADA"
     gene            complement(7871..8449)
                     /gene="pyrR"
                     /locus_tag="VE646_05205"
     CDS             complement(7871..8449)
                     /gene="pyrR"
                     /locus_tag="VE646_05205"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012798319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HZD79419.1"
                     /translation="MGGGGRLLDEAEIRRSVTRIAHEILEKNKGAARTVLVGIANRGD
                     DLARRLASEIERIEGQPVPVGVLDITFYRDDIGMRAEAPEVHETRIGFDINDRTVVLV
                     DDVLYTGRTIRAAMDALVDFGRPTAIQLAVLVDRGHRELPIRADYVGKNLPTRRGEDV
                     RVALKELDGEDAVYVEGRPGSHAGPGGTGDDA"
     gene            8698..9807
                     /locus_tag="VE646_05210"
     CDS             8698..9807
                     /locus_tag="VE646_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HZD79420.1"
                     /translation="MGLSMAERKAVTKEMARRYARASKKDKGRMLDELCELTGWTRRH
                     GIRALAQAVHPPPRPSRAPRARVYGPEVLEPLLVVWATLNGPAGKRLAPFMGEIVTAL
                     ERCGELQVDPEVRTKLLSMSAATIDRALAPERARLRVKGRSGTKPGTMLKRQIPIRTF
                     AEWDDARAGFCEVDLVAHDGGDPAGEFCQTLDLTCVATGWTECRAVRNKAQRWCFEAL
                     LEIEDSLPFALVGLDSDNGSEFINDQLFRYCTERGITFTRSRPYRKNDNCFVEQKNWP
                     VVRQNVGYGRYDTPAELEVLNELYRHLSPYVNFFQPQMKLVSKTRRGAKVSKHFDRAR
                     TPYQRILESPHVSGDTKGPSKNNYTLVVLACAWIV"
     gene            complement(9768..10985)
                     /locus_tag="VE646_05215"
     CDS             complement(9768..10985)
                     /locus_tag="VE646_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HZD79421.1"
                     /translation="MPVVDEVLVRKFDTVFPHLDERQRRIYAAAEARALGRGGIARVV
                     EASGLSRSTVQRAMGELDRGFEVSPRIRRPGAGRKPIVKANPALVRALEALVDPGARG
                     DPESPLRWTIKSTRQLAHALTDQGHAVSHSAVADLLHALGYSLQANIKTQEGAAHPDR
                     DAQFSYLAREVGRRLRRGEPVISVDTKKKELVGQFKNAGQTWRPKGDPEEVAMHDFAD
                     PALGKAIPYGVYDVGANTGWVVVGTDHDTAEFAVATIWSWWETVGGPSYPEATRLLVC
                     ADGGGSNGYRVRLWKVELHRLAAAAGLDITVCHFPPGASKWNKIEHRLFSHISMNWRG
                     RPLTSHEVIVDLIGSTTTRQGLKVRASLDPRSYPTRTKITDEQLAAVPITRHRFHGEW
                     NYTIHAQARTTRV"
BASE COUNT         1631 a         4172 c         3838 g         1633 t
ORIGIN      
        1 gccctgcagg gcgctggtga cgcggacgta ctcctccagg ctggagccgc ccaccgaggc
       61 gatcaccggg atcctcagcc gtcgcagccg gggcaggtcc tcggccatga agtcgcgtac
      121 gcccgggttc tgacccacgg cggtgacgat gccggcgcgg ctctcggcca cgcgcggggt
      181 gggggcaccc cgggagggac cgtaggtgag gcttccggtc accacggccc cgagggcccg
      241 atggtccacc agcccggaga gctccctgcc cgtcccggag cagcccgagg ccgccagcac
      301 cgggaccggg agccgcaggc cccgcagatc gacggccagc gaccggctca ccccggccac
      361 gcggtcaccg ccgggaagcc ctcaggcggg gtgggcacgg tccgcggctc gtcgttcatc
      421 caccgatccc agaagatgcg ggccgggttg aacacggggc cttcggtgca ggcccgcagg
      481 ttgtcgtagc cggagccgtc cttgcggatc accgggacca cgcacgtgta gcagaggccc
      541 agcccgcagg ccatctccgc ctccaccgcc acctgcgcgg gcagccgccg ctcggcgcag
      601 aaccccgcca ccgcccgcag cacgctccgg ggcccggccg cgtagaccac ctgagccccc
      661 gtggacgcgg tcacggcggg cagagcctcg gccacggtcc cctggctgcc cagcgagccg
      721 tcccgggtca cgaccgtgac cgtctgcgac agccgcttgc cctcgatcgg cctgaagacc
      781 cgggactcct ccgtcccccc gatcagcatg tccacccgct tgccccgggc ccggagctcc
      841 tgggccagga agtagatctg ggcggtgccg tgcccctcgg agaccaacag gcagttggtc
      901 agccgctgcg ggtacgcgaa ggacttgccg agcgggccga tcacgtccac gaagcggtgg
      961 gccttcatct cggccagcca ggccgtcccc gggccccggg ggtcgaacac gaactcgagg
     1021 gtaccggccc acccgccccg gcgagacgcc tgatggatcg agaagtgccg gcggaggatg
     1081 aagccccgat cagggggcat ggccaccgag atgaactgtc cgggccgggc cttctccgcg
     1141 atctggggcg ccaccagcgt catggagtgg aacccgccct gccggcgaac gttcaggatc
     1201 tcggcgcggg cccgcctcac cccggggacc tccccgcgag ctcgtggtag tcctggatcg
     1261 cgagcggctc ggcggggtcc tcgcgcagag cctcgatccc gcgcagggcc gccagcacgc
     1321 cgggcagcgt ggtgatgcag gggatccccg cccgcgcggc cgcggtgcgg atgtagtacc
     1381 cgtcggtccg ggggctccgc ccgaacggcg tgttgatcac caggtccacc tcgccgccgg
     1441 cgatcatatc ggccacgttg ggactgccct cggacacctt gtccacccgc tccaccgcga
     1501 tcccggcgcg ctccaggacc cccgcggttc cccgggtggc caggagccgg aacccgagct
     1561 cggacagccg ccgggcgggg aaggcgatgg cccgcttgtc gcggttcgcc accgagacga
     1621 acacggtccc ggccgcgggc aaggtggcgc cggaggacac cagggccttg gccagggccg
     1681 accccgagtc ctggtcgatc cccatgacct cgcccgtgga gcgcatctcg ggacccagca
     1741 cggtgtccac gccggggaac cgtccgaacg gcagcaccgc cgccttcacg cacgtgtacg
     1801 gcaagcgccg gtagtgggcc ggatcggcgg gaaccaggcc gagcgcggtc tgctcgcgca
     1861 ggctccggcc cagcagcacc agggtggcca ccttggccag gggcacgccg gtggccttgg
     1921 atacgaacgg cacggtgcgg gaggcgcgcg ggttggcctc gagcacccag atccgctcgt
     1981 ccttcaccgc gagctgcagg ttcatgagcc cgcggacccc caggcgctcg gccagggccc
     2041 gggtgattct ctccacctcg tccagctcgt cgtccgacag ggtgggcggg gggatcgcgc
     2101 acgacgagtc gcccgagtgc accccggcct cctcgatgtg ttccatcacc gcgcccacga
     2161 acacgtcggt gccgtcggcc acggcgtcca cgtcgatctc gatcgcgccc tccaggaacc
     2221 ggtcgattag caccgggtgg tcggggctgg cctcggcggc ggagcggacg aagcgctcca
     2281 gctcgtcctc cccgtagacg atctccatgg cgcggccgcc gagcacgtag ctcgggcgca
     2341 ccacgacggg gtagccgatc cgccgggcca cctcccgagc ctcctccaga tcgcgggcct
     2401 cgccgtgcgg cggggccggg atgccgagct cggcgcacag cgtggcgaac ttcccgcggt
     2461 cctcggccag atcgatggcc tcgggcgcgg tgccgagcac ctcgaacccg tgctcctgca
     2521 gcgtgggggc cagcttcagc ggggtctggc cgcccagctg cacgatcacc cccaccgggc
     2581 gctccacgcg gcacacggcc agcacgtcct cggccgacag gggctcgaag tacaggcggc
     2641 tgctggtgtc gtagtcggtg gagaccgtct cggggttgct gttcaccatc acggcctcgt
     2701 agcccgcctc gcggagcgcg taggcggcgt gcacgcaggc gtagtcgaac tcgatgccct
     2761 gcccgattcg gttcggcccc gcccccagga tcagcacccg cgggcgctcg gccggacgga
     2821 cctcgctctc ctcctcgtac gtggagtagt ggtacggcgt ggtcgcaggg aactcccccc
     2881 cgcacgtgtc caccgtcttg tacacgacct ctaccccgag ttcgctccgc ctggcccgca
     2941 cctccccctc cgtcgccccc gtcagccgcc cgattcgccg atcggagatc cccacccgct
     3001 tcgcctccag cacctcgccg gccccgagcg atccgagcgc ccgcccctgg agcgcagacg
     3061 cctcctccac cacctgcgcg atctgatcca cgaaccatgc atcgatcccc gacctccggg
     3121 cgatcccttc aaccgagagc ccctcccgca gcgcgagctc caccaggtgc aaccgcccct
     3181 ccgaagggat ccggagcagg tcgaggagcg agtgggccgg tgcggaaggg ggcggctcgt
     3241 cgtggaggat gcccctgagc ccgagccatc cgaggtcgaa cccgtctcgc tccatcgacc
     3301 gccacgcctt ccccaacgcc tccttgaagg tccgcccgat ggccatcacc tcgcccaccg
     3361 acttcatcgt ggtgctcaag gtgggatcgg cctcggagaa cttctcgaag gcgaagcgcg
     3421 ggatcttcac cacgacgtaa tcgagggtcg gctcgaaggc ggccggcgtc tggccggtga
     3481 tgtcgttggt gatctcgtcc agccggtacc ccacggccag tagggcggcg atcttcgcga
     3541 tggggaaccc ggtggccttc gatgcgagcg ccgacgaacg cgacacgcgg gggttcatct
     3601 cgatcagcac ctggcggccg tcgcgcgggt cgacggcgaa ctggatgttc gatccgccgg
     3661 tctccacgcc gatcgcccgg atgcacgcca gcgccgcatc gcgcatctcc tggtactcgc
     3721 gatcggtcag cgtctgttgc ggcgcaacgg tgatggagtc gccggtgtgc acgcccatgg
     3781 gatccacgtt ctcgatcgag cacacgatca cggcgttgtc ggccccgtcg cgcatcacct
     3841 cgagctcgaa ctccttccat ccggcgaccg actcctccac caggacctcg tgtgccgggc
     3901 tctcggccag gctccgtgcg gccagctcgc gcaggccggc cgggccgtcc gcgaagccgc
     3961 tccccccgcc gcccagcatg aacgagggcc ggacgatcac cgggtagccg atcgcatcgg
     4021 ccacggccag ggcctcgtcc acgccgtggg cgatcccggc ccgcggcacc gggagaccgg
     4081 cctcggtcat cgcgcgctgg aagccgctgc gatcctcggc ccggtggatc gcgtccaggc
     4141 tggccccgat cagcgagacg cccaggcggt ccagctcgcc cgactcggcc agcgccaccg
     4201 ccacgttcag cccggtctgc ccgccgaggg tgggcaggat cgcatcgggc ttctcgcgac
     4261 ggatcacgct cagcacggac tcgggcgtga gcggttcgat gtaggttcgg tcgctgaact
     4321 ccgggtcggt catgatcgtc gccgggttcg agttgaccag cgacacctgg aagccctccc
     4381 cccggagcac cttgcaggcc tgggtgcccg agtagtcgaa ctcgcaggcc tggccgatca
     4441 cgatgggccc cgagccgatc accaggatcc ggtccatgtc cgtgcgccgg ggcatcagtg
     4501 cgcccccatc agctcgcgga accggccgaa caggtgccga gcgtcgtgcg gcccgggagc
     4561 ggcctcgggg tggtactgca cgccgaaggc cggcacgtcg agcgcccgca ggccctccag
     4621 cgtgccgtcg ttcaggttca cgtgggtcat cgccacccga tcccccagcg aggccgggtc
     4681 caccgcgaac ccgtggttgt ggctggagac ctccacgcgc tcggtggcca ggtccttcac
     4741 cggctggttc gcgccgcggt ggccgaactt catcttgaac gtccgcgtcc cgagcgccag
     4801 ccccagcagc tggtggccca ggcagatccc gaacacgggc agccggccca gcagggcccg
     4861 cgtcgcctcc acgccgtacc gggtggccgc cgggtcgccg gggccgttgg agaggaatac
     4921 gccgtcgaac ccgtccccca ggacctcgag cgccggggtc cgggcgggga agaccgtggt
     4981 ctcgcatccg gcggccgcca gcaggcgcag gatcgtgcgc ttcatgccga agtcgtaggc
     5041 ggcgacccgg aacacccggc cgaggtccgt cgcggccggg cccaccaccg agcgggcctc
     5101 gaagggctcc tcggcgctca cccccgcggc caggtcggcg ccctccatcc cctgcgtcgc
     5161 ccgcacgcgc tcgaccaggg agtgggggtc gaggtcctct gtggacagcc cgcaccgcat
     5221 cgccccttcg ctccgcagcc gaagggtgag ccggcgggtg tcgatgccct cgatccccac
     5281 caccccggcc gccccgagct cctgcgccag cgtccgctcg ccccgccagc tggaggcccg
     5341 tcgggacgcc tcgcgcaccg cgaacccggc cacccgcacg cgtccggatt ccgggtccag
     5401 gccgttcgtg ccgtagttgc cctggtgggg agcggtcatg accaccacct gaccggcgta
     5461 cgaggggtcg gtcaggacct cctggtagcc cgacatcgcg gtgttgaaca ctgcctcccc
     5521 gaaggtctcg cccccggccc cgaaggcccg gccgcggaac cggctcccgt cctcgagcac
     5581 caggagggcc tggcgggcgg cccgcgatcc cttcgagccc accctcatct cagcgctgcg
     5641 gcttcccgtc gcagaccacc agctcgccgc ggtgcaccgt gtggattacc cggccccgca
     5701 gccgccgtcc caggaaggcg gagttgcggc tgcgcgattg gaacggtggc tccaccaccc
     5761 actccgcctg cgggtcgaag gccaccaggt gggccggacc accgggctcc actgggcccc
     5821 cgtgctcgct cagcccgagc agccgggccg gggccgtcga caggacctcg atcgcccgct
     5881 cgagcgtcaa gatcccgggc tccaccaggt gggtgaggat cgccgccagg gcggtctcca
     5941 ggccgatcgt gccgggcggc gcgtggtcga actccgcctc cttctcctcc accgcatggg
     6001 gcgcgtggtc ggtgcccacc acgtcgatcg tgccgtcggc gagcgccgcg cgcagcgcgg
     6061 cccggtcctc cgcggtgcgc agcgggggct tcaccttgcg gttcgtgtcg tagttcagca
     6121 ggtcctcgtg cgtgaacgtg aggtggtgcg gggtggcttc ggcggtcacc cgcaggccct
     6181 cggccttggc ctggcgcacc agctccaccg agcccgcggt ggataggtgc agcagatgca
     6241 ggcggccccc ggtgagccgg gccagcgcga gatcccggta cacgatcacc tcctcggcct
     6301 cggccggctc gccggccagc cccagcgtgt aggagtgcac gccctcatgc atctgggtgt
     6361 cctcggacag cgccgcgtcc tcgcagtgct cggccagcac cacgtcgaac gccttcgcat
     6421 actcgagcgc gttgcggagc actcgggacg tgggcacgca ccgaccgtcg tcgctgaaca
     6481 tccgcacgcc gatggccgac atctcgccga tcgcggccat ctctgccccc tccaggccct
     6541 tggtgatggc tcccaccggg gagacgtcgc agagccccgc ccggagggcc agcgagcgga
     6601 cctcctcgat caccgccgcc gtgtccgcca ccgggtcggt gttggccatg gccgtgatgg
     6661 cggtgtagcc gcccgcgacc gcggcgcgcg agcccgtctc cacggtctcc ctatcctcgc
     6721 ggcccggctc ccgtaggtgc gtgtgcggat ccaccaggcc gggcgccagc acgaggccgg
     6781 tgcagtccag gacctcggtc cccgccgggg ggctcgcgtc ctcgccgatg gcggcgatcc
     6841 ggccgcgctc gaccagcacg tgccggacgg cgtcggtggc cgaggccggg tccaccagcc
     6901 gggccccccg gaacaggatg cgatcaggca tcggcggccc cctctccttc gtccgagctc
     6961 gagcccgagc ccccgagcag caggtagagc agggacatcc gtaccgcgat gccgttcgtc
     7021 acctgctcct ccatcaccgc ctggggcaga tcggccacgt ccgagtcgat ctcgaccccg
     7081 cggttcatcg gtccggggtg catgatcagg gcgccctccg ggagcacccc cacccgctcc
     7141 ctggtgaggc cgaacagccg cgcgtactcc cgcacgctgg ggaagtactg ctggcgctgg
     7201 cgctcgcgct ggacccggag catgtagcag acgtcgagct tggggagcac ggcgtcgatg
     7261 tcgtaggaga cctgcacgcc ccaggccgga gcgtcgggcg ggatcagcgt gggtggcccc
     7321 accagcgtga cctcggcccc catcttcacc agcgcgaagg acagggaccg cgcgacacgc
     7381 gagtgcagga cgtcgcccat gatcgcggtg cggaggccct cgaaccggcc caggcgctcc
     7441 cggatcgtat acaggtccag cagggcctgg gtggggtgct cgtgggtgcc atccccggcg
     7501 ttcaggacgc tgccccggat ccaccgggtg agggtgaagg gcgacccgct ggaggagtgg
     7561 cggatcacca cggcgtcggc ccccatggcc tgcagggtga gggcggtgtc cttcaggctc
     7621 tcccccttgg ccacgctggt gccgctggtg gtgaagttga tcacgtcggc cgagagccgc
     7681 ttcgcggcca gctcgaacga gatccgggtg cgggtggagt tctcgtagaa caggttcacc
     7741 acggtgcgcc cccggagggc cgggaccttc ttgatgaccc gcgtgcccac ctcgcggaag
     7801 gactcggccg tgtccaggat ccgcacgatg tcttgggcgg acacgtcgtt gatcgacagc
     7861 aggtgcttgt tcatgcgtcg tctcccgtcc cgcccggccc cgcgtgcgag cccggccgtc
     7921 cctccacgta gacggcgtct tcgccgtcga gctccttcag ggccacccgg acgtcctcgc
     7981 cgcggcgggt gggcaggttc ttgcccacat agtcggctcg gatcggcagc tcccggtgac
     8041 cccggtccac cagcacggcc agctggatcg cggtggggcg gccgaagtcc accagcgcgt
     8101 ccatcgcggc gcggatggtg cgtcccgtgt acaggacgtc atccaccagg accacggtgc
     8161 ggtcgttgat gtcgaacccg atgcgggtct cgtggacctc gggggcctcg gcccgcatcc
     8221 cgatgtcgtc ccggtagaag gtgatgtcca ggacgcccac gggcaccggc tggccctcga
     8281 tccgctcgat ctcggaagcc aggcgccggg ccagatcgtc gccccggttg gcgatgccga
     8341 ccagcacggt gcgggcggcg cccttgttct tctccaggat ctcgtgcgcg atgcgggtca
     8401 cggagcgccg gatctcggcc tcgtccagca gccgcccgcc gccgcccatc acggaagaac
     8461 gcccaaccgc tcccgcggca gggcgccctg gtcgtgcacc atcgttcctc gcctcccttc
     8521 ccggcctcgc gggaccggct taaaggggaa gttcccggcg caggatacgt cgcggagggc
     8581 ctcgcggcaa cgggccgagt tgactcacca gaaatgtcct cgaaacggct tcattgcctc
     8641 acctggaatg gtcctcgaag gatccacgct cctgcctgat cgggcaggag gggtgcggtg
     8701 ggcctttcga tggcagagcg caaggcggtg accaaggaga tggccaggcg gtacgccagg
     8761 gcctcgaaga aggacaaggg ccggatgctc gacgagttgt gcgagctcac cgggtggacc
     8821 aggcgccacg gcatccgggc gctcgcccag gcggtccatc caccgccccg gccgtccaga
     8881 gcgcccaggg ctcgcgtcta tgggccggag gtcctggagc cgctcctggt ggtgtgggcc
     8941 accctgaacg gcccggccgg caagcgcctg gccccgttca tgggcgagat cgtgaccgcc
     9001 ctggagcgct gtggcgagct ccaggtcgat cccgaggtcc ggaccaagct gctgtcgatg
     9061 tccgcggcca cgattgaccg ggccctggcg ccggagcggg ctcgcctgcg ggtcaaagga
     9121 cgctccggga ccaagccagg gaccatgctc aagcggcaga tccccatccg aaccttcgcc
     9181 gagtgggacg atgccagggc cgggttctgt gaggtggacc tggtggccca cgacgggggc
     9241 gatccggccg gcgagttctg ccagaccctc gacctgacct gcgtggcaac gggatggacc
     9301 gagtgccggg cggtgcgcaa caaggcccag cggtggtgct tcgaggccct gctggagatc
     9361 gaggactccc tgccgttcgc cctggtgggg ctggactcgg acaacggcag cgagttcatc
     9421 aacgaccagc tgttccgcta ctgcaccgag cggggcatca ccttcacccg ctcccgcccc
     9481 taccgcaaga acgacaactg cttcgtggag cagaagaact ggccggtggt ccgccagaac
     9541 gtgggctacg gccgctacga cacccccgcc gagctcgagg tgctcaacga gctctaccgc
     9601 cacctcagcc cgtacgtgaa cttcttccag ccccagatga agctcgtctc caagacccgc
     9661 cggggggcca aggtgagcaa acacttcgac cgggcccgca ccccctacca gcggatcctg
     9721 gagtcccccc acgtgtccgg cgacaccaag ggcccgtcca agaataatta cacccttgta
     9781 gttctcgcct gtgcgtggat cgtgtagttc cactcgccgt ggaaccggtg cctggtgata
     9841 ggcaccgcag cgagctgctc gtccgtgatc ttggtgcggg tggggtacga gcggggatcg
     9901 agcgatgcgc gcaccttgag cccctggcgg gtcgtcgtgg agccgatgag gtccacgatc
     9961 acctcatggc tggtgagcgg acggcctcgc cagttcatgg agatgtggga gaacagtcga
    10021 tgctcgatct tgttccactt cgacgctccc ggagggaagt ggcacaccgt gatgtcgaga
    10081 ccggcagcgg cggccaggcg atgcagctcc accttccaca gccggacccg gtacccgttg
    10141 gacccccctc cgtccgcgca caccagcagg cgggtggctt ccgggtagga agggccgccc
    10201 acggtctccc accagctcca gatggtggcc acggcgaact cggcggtgtc gtgatcggtc
    10261 cccaccacca cccacccggt gttggcgccg acgtcgtaca cgccgtaggg gatggccttg
    10321 cccagggctg gatcggcgaa gtcgtgcatg gcgacctcct cgggatcccc cttggggcgc
    10381 cacgtctgac ctgcgttctt gaactgcccg accagctcct tcttcttggt gtcgaccgag
    10441 atgaccggct cgccccggcg cagccgacga ccgacctccc gagccaggta ggagaactga
    10501 gcgtcccgat cggggtgagc ggcgccctcc tgggtcttga tgttggcctg cagggaatac
    10561 cccaaggcgt gcagcaggtc ggccacggcg gaatggctca ccgcgtggcc ctgatcggtg
    10621 agggcatggg cgagctgacg ggtgctcttg atggtccacc gcagggggga ctccggatcg
    10681 ccccgagccc ctgggtccac caacgcctcc agggcccgga ccagggcggg gttggccttc
    10741 acgatcggct tgcgtcccgc tcccgggcga cggatgcggg gtgagacctc gaacccccgg
    10801 tccagttcgc ccatagctcg ctgcacggtg gatcgggaca ggcccgatgc ctcgactacc
    10861 ctcgcgatcc cccctcgtcc cagggctctc gcttccgccg ccgcgtagat ccggcgctga
    10921 cgttcgtcca agtgggggaa cacggtgtcg aacttgcgca cgagcacttc gtcgacgacc
    10981 ggcacacggc cagtctactc cacgcctggc ctgatagcgt cacttattgt gctacagctc
    11041 ccaaggcctg cctgacccgg atctacctgc agctcaaccc ggccgagctc aagcggcgga
    11101 tggccgactg ccagtcgcgg ctgctgcaaa tcaaccggac caaacccgat cggagcaagg
    11161 aggtgagacg acccggacac ccctccgccg ggaggaagtt ctcctggacg gagatttcga
    11221 ggacatcttt ggtgaggcaa ccggaggtgg cgccgaggac atcttgacgt gagt
//
LOCUS       DASQQQ010000123         3183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_65463, whole genome shotgun sequence.
ACCESSION   DASQQQ010000123 DASQQQ010000000
VERSION     DASQQQ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65463"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..824
                     /locus_tag="VE646_05220"
                     /pseudo
     CDS             33..824
                     /locus_tag="VE646_05220"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(875..2329)
                     /locus_tag="VE646_05225"
     CDS             complement(875..2329)
                     /locus_tag="VE646_05225"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD79422.1"
                     /translation="MKGTLDRARDALRSSDFRRLFAIRLVSQSADGLFQAALVASVVF
                     SPERQSTTTGFAIATLVISLPFSVIGPFTGVFIDRWPRRRILQVAPWLRTALVWLVLF
                     DPVRAPLPFYAGALWVLSVNRFYLATAQAIVPRLVPTEDLLMANSLATVGGTVALLAG
                     VFVGGWVADLVGNLPVIVVAGAMWLIASGVASRITTALSPHQVPSSAVGDELRRVAGE
                     FVDGLERLLRSPRALGPITSISLDQMGQGVVLVLSLIVFRERFEQGVGSFSNLIGAGG
                     IGVLLGILTVGKLEERFPKERIVAGAFYAGAGCLLLVSTYITGWSVLLASFAVGLTFA
                     WKKIPIDTMVQEAIPDGYRGRVFAVYDVLYNLSRVVAAFLAIPLLPALGPARSVGLIG
                     ALFLLWAPVLPRWVRRASDLVIRFYEGGRAEEWPRSVVWGGVEEDVRVQRSWLEERNG
                     ERRTRYRLAMEDGTVIEVSRAEGAPSWSIDRELS"
     gene            complement(2411..>3183)
                     /locus_tag="VE646_05230"
     CDS             complement(2411..>3183)
                     /locus_tag="VE646_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018349013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HZD79423.1"
                     /translation="STLREVEADVVVSYLPVGSEEAAKHYAQAALDARVALVNCLPVF
                     IASDRTWAKRFVQAGVPIVGDDIKSQVGATIVHRILTKLFEDRGVKLNRTYQLNFGGN
                     MDFMNMLERERLASKKRSKTQSVQSQMAKPLDGRDIHIGPSDYVPWLEDRKWAQIRLE
                     GQAFGDVPLNVELKLEVWDSPNSAGIVIDALRCAKVAKDRGVAGPLIGPSAYFMKSPP
                     VQFTDDSAREMVEEFILGNGASKRADWTAFFDSQPAEA"
BASE COUNT          504 a         1120 c         1097 g          462 t
ORIGIN      
        1 acaaccccac ccggcagagc ttctatgccg agatggtcgg cgctcgcgat ctgacgaacg
       61 cggtgagcct gaacagcgcc gtgatgacgg ccacccgcat cgtgggaccg gcggtggccg
      121 gcctggtgat cgcggtggcg ggactggccc ccgcgttcct gctgaacgcg gtctcctaca
      181 tcgcggtcat cacggcgctc gcggcgatgc ggccccagga gctgatccgc gacggcatgc
      241 aggcccggcg tagaggtcag gtgcgggagg ggatcgcata cgtgtggcgg acgccggcgc
      301 tgcggcagcc gctgatctgg atggcgatcc tctacacgtt ctcgttcaac ttcgcggtgc
      361 tgttccccct cctcgccgag gacaccttcc agggcaacgc cgggacctac ggcacgttgc
      421 tgtcggccct cgggatcggt tcgctgatcg gcgcgctgct gatggcacgg caggcccgac
      481 cgacgcccag gcgactggcc accgctggtg cggtcttcgg ggccgtgtcg atcgtcgcgg
      541 cgttgatgcc caccctggac gcgacgctcc tggttctggt gccgatgggc cttggcagca
      601 tgctgttcat gatcctggcc aactccaccc tgcagctcgc gtcccggccc gagatgcggg
      661 gccgagtgat ggccctgtac ggcatcgtgt tcctgggcag cacccccttc ggtgcgccca
      721 tcgccggatg ggtgggggag catctgggtg cacgggccgg tatcgcgttg ggcggggcga
      781 tcgcgctggt caccgggctg gccgggctgt gggcggtgct ccgccgcgga gcgctcgcgt
      841 ccccgggcac cggagcccgg gaggacacgg acggtcagga tagctcgcgg tcgatcgacc
      901 aggaaggtgc gccctcggcc cgcgagacct cgatcaccgt cccgtcctcc atggccagcc
      961 ggtaccgggt gcggcgctcg ccgttgcgct cctcgagcca gctgcgctga acccggacgt
     1021 cctcttccac gccgccccag accaccgagc gcggccactc ctccgcccgt ccaccttcgt
     1081 agaaacggat caccaggtcg gaggcgcgcc ggacccagcg gggcaggacc ggtgcccaca
     1141 gcaggaacag cgcgccgatc agccccacgg agcgggccgg tccgagggcg ggcagcaggg
     1201 ggatggccag gaaggccgcc accacgcggg agaggttgta cagcacgtcg tacacggcga
     1261 agacccggcc ccggtacccg tcggggatcg cctcctgcac catggtgtcg atggggatct
     1321 tcttccaggc gaaggtgagc cccacggcga agctggcgag cagcaccgac caccccgtga
     1381 tgtaggtgga gaccaggagc aggcagccgg cgccggcgta gaaggcgccg gccacgatcc
     1441 gctccttggg gaagcgctcc tccagcttgc ccacggtcag gatgcccagc aggaccccga
     1501 tcccgcccgc gccgatcagg ttcgagaacg atcccacgcc ctgctcgaac cgctcccgga
     1561 agacgatcag ggacaggacc aacacgaccc cctggcccat ctggtccagg ctgatcgagg
     1621 tgatgggacc gagcgctcgg gggctccgca gcaggcgctc gagcccgtcc acgaactcgc
     1681 cggccacccg gcggagttcg tcgcccacgg ccgacgaggg cacctggtgg ggcgagagcg
     1741 cggtggtgat ccgtgaggcc acgcccgacg cgatcagcca catggcgccg gccaccacga
     1801 tcaccggcag gttgcccacc agatcggcca cccaccctcc cacgaacacc ccggccagca
     1861 gggccaccgt tccgcccacc gtggccaggg agttggccat cagcaggtcc tcggtgggca
     1921 ccagccgagg gacgatggcc tgggcggtcg cgaggtagaa ccggttcacg gagaggaccc
     1981 acagggcgcc ggcgtagaag ggcagcggcg cccggaccgg gtcgaacagc accaaccaca
     2041 ccagtgccgt tcgcagccag ggcgcgacct gcaggatccg acgccggggc caccggtcga
     2101 tgaacacgcc ggtgaagggc ccgatcacgg agaagggcag cgagatcacc aaggtggcga
     2161 tggcgaaccc ggtcgtggtg ctctgccgtt cgggcgagaa caccaccgag gccacgaggg
     2221 cggcctggaa cagcccgtcc gccgactggc tcacgagccg gatggcgaac agacgccgga
     2281 aatccgagct gcggagcgcg tcgcgagccc gatccagggt gcccttcatg ctcttcagtc
     2341 ttccaccccg aggggagcga catcgacgcg ccggtgggtc ccggccgtcg ggtcgtccag
     2401 gagaggtggc tcaggcctcc gccggctgcg agtcgaagaa ggcggtccag tcggcccgct
     2461 tgctggcccc gttgccgagg atgaactcct ccaccatctc ccgcgccgag tcgtcggtga
     2521 actgcaccgg cggcgacttc atgaagtagg cgctcggtcc gatcagcggt ccggccaccc
     2581 cacgatcctt ggccaccttc gcgcaccgca aggcatcgat cacgatgccg gcggagttcg
     2641 ggctgtccca cacctcgagc ttgagctcga cgttcagcgg gacatcgccg aaggcttgac
     2701 cctcgagccg gatctgcgcc cacttgcggt cctcgagcca cgggacgtag tcggaggggc
     2761 cgatgtggat atcgcgtccg tccagtggct tcgccatctg gctctgcacc gactgggtct
     2821 tcgatcgctt cttggaggcc agccgctcac gctccagcat gttcatgaag tccatgttgc
     2881 cgccgaagtt cagctggtag gtccggttca gtttcacgcc gcggtcctcg aagagcttgg
     2941 tcaggatccg gtggacgatg gtggccccca cctggctctt gatgtcgtcg ccgacgatgg
     3001 gcacccctgc ctgcacgaac cgcttggccc aggtccgatc ggaggcgatg aacacgggca
     3061 ggcagttcac caacgccacc cgcgcgtcga gcgcggcctg ggcgtagtgc ttggccgcct
     3121 cttcgctgcc cacggggagg taggacacca cgacgtccgc ctccacctca cgcagcgtcg
     3181 agg
//
LOCUS       DASQQQ010000124         6117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_258295, whole genome shotgun sequence.
ACCESSION   DASQQQ010000124 DASQQQ010000000
VERSION     DASQQQ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258295"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VE646_05235"
     CDS             complement(<1..584)
                     /locus_tag="VE646_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79424.1"
                     /translation="MTRPLSPTAFLRSLRRTRLGGPIEYAGQALGGLARPWLLRSGAT
                     PEEEGRPLPGDELVAHPNWQATRAVSIDATPARIWPWVAQMGYGRGGWYGWNPLERED
                     TGAFRLLPNLPATKVGDVLLDGPGCDETKGAFEVMTVEAPRTLVLHSARDPLTGREVD
                     PAHKPRLLIDTAWSFCLDPIDRRRTRLLARTRIQ"
     gene            800..2764
                     /locus_tag="VE646_05240"
     CDS             800..2764
                     /locus_tag="VE646_05240"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HZD79425.1"
                     /translation="MIGERLDVWDLLAPGMLISNLPWMLVLAWFSRRLLGLRRRSLWS
                     SAAAGLIGWLIGSGIALLSIRGDVQGHYDAVWRIGVVLSVLFTMLATVGVELLARHRE
                     EAFSLPGTTIPHPVRSVRALLARVGRYIQVLRIAARHGLRMRPGHPPDRLPAREVRAA
                     LEECGGMFVKLGQMLATRPDVVGPSLAEELTGLQEDAPPAPTEDVDRLLQEEFGHPVG
                     EVFERFDAEPIAAASIAQAHRARLGSGEEVIVKVQRPGVASLVERDLSILLRLTRRAE
                     RRARWAESYGVAKVAEEFAAGVIKELDFTIEARSVEEIGAALGDTPEIRVPHVFTELS
                     TPRVLVLEFLQGVSVGQRERVRELGVDHEKLADVLLRSTISLVVGGERFHADPHPGNV
                     MVLADGRLGLLDFGSTGRLDAIERASVVDMLTAIAQNDPELLRDAVLAIAVVRRRIEE
                     RALDRALAGFMARHLGRGAAPSVAMLQELAQMFLAMGIALPSTTTMLFRMLVTLEGTL
                     TTLCPGYELIGAARDLAPELIRERLAPSRWQEQAREELMGLMPVLRRAPRHLDRVATI
                     LERGELATRVSLFATEDDQRVLRALVNRTLLVLTGGAVGVVAVGLLAVPGGPTVSGVV
                     SLFDVLGYMGLFLSAVLLLRVTVAALIEGG"
     gene            2961..3395
                     /locus_tag="VE646_05245"
     CDS             2961..3395
                     /locus_tag="VE646_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79426.1"
                     /translation="MRLSWRDGLATLLVGAGAILYALWLADAEVAGFSGVRPLAGLVL
                     GLGLAASVTAVVYGVGAGLLRASKVYLTVASVIGLTALVAGTVALVGASETMLAALVA
                     STVALWAISTARHGLIAEHAGGRVAPTAAGGVGARAPRGAHG"
     gene            3435..3656
                     /locus_tag="VE646_05250"
     CDS             3435..3656
                     /locus_tag="VE646_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79427.1"
                     /translation="MRRGWIVAGVVLAAAYLARRLAARSGALSWEQMIERMPDTAPPK
                     WVFNNIQAIRENTEQILGRLRSEPATAPS"
     gene            3665..4024
                     /locus_tag="VE646_05255"
     CDS             3665..4024
                     /locus_tag="VE646_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79428.1"
                     /translation="MAIGAASLVALWGVAHAIPTRAVVAGFGPISIDNRRIILQEWLV
                     EAFAMWGIAALVIGVTVLDADGEATAWVCRGSAAALVAIGVLTAFTGGRTQVVWFKIC
                     TVLLTTTAALLLAASFL"
     gene            4456..4617
                     /locus_tag="VE646_05260"
     CDS             4456..4617
                     /locus_tag="VE646_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79429.1"
                     /translation="MPEVEVDRNEDVAWACGCGPAMRQMAAACCGEPEPESTGSEEQE
                     QTPRVQGGA"
     gene            4677..4925
                     /locus_tag="VE646_05265"
     CDS             4677..4925
                     /locus_tag="VE646_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007296069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZD79430.1"
                     /translation="MLLCGIFMVRMGGRRGMGGMCGWWRGRGYHDQTDAAVAQRSSDH
                     DALVRPSARGILEERFARGEISEEEFEGRKQVLARNPS"
     gene            5148..5813
                     /locus_tag="VE646_05270"
     CDS             5148..5813
                     /locus_tag="VE646_05270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZD79431.1"
                     /translation="MSRSSGPGASLEKAPARPARVSSGELFASYQVRIRRYISSMVHD
                     PVEADDLTQDVFLQVHRKLDSVRDPDAVVSWLYRIATHLCYDRFRRSSREPRTDPLDV
                     GGSGGGRGLGDIADQLRLDRVLEQREMSACVRSYIDGLSTQYRQVILLHDLEEVSNPQ
                     IAQMLGASLDTIKIRLHRARRKLQVALGEHCLLSLDEQGVLVCEPAEVTTAATSGSVR
                     GSP"
BASE COUNT          828 a         2018 c         2264 g         1007 t
ORIGIN      
        1 atctggatcc gggtcctggc caggagccgc gtccgtcggc gatcgatcgg gtcgaggcag
       61 aacgaccatg cggtgtcgat caggagacgg ggcttgtggg cggggtccac ttcccgcccc
      121 gtgaggggat cccgcgccga gtgcaagacc agggtccgtg gggcctcgac ggtcatgact
      181 tcgaaggctc ctttggtttc atcgcagccg gggccgtcca gcaagacgtc gccaaccttc
      241 gtggccggta ggttcggaag gagccggaag gcgccggtgt cctctcgctc cagtgggttc
      301 cacccgtacc agccaccgcg gccatagccc atctgcgcga cccagggcca gatcctggcc
      361 ggggtggcat cgatgctgac ggctcgcgtt gcctgccagt tcgggtgggc aacgagctcg
      421 tcaccgggga gtggtcgccc ttcctcctcg ggggtcgctc ccgagcgcaa gagccatgga
      481 cgggccaacc ctcccagggc ctgtcccgcg tactcgatcg ggccgccgag cctggttcgg
      541 cgcagggaac ggaggaacgc cgtcggagaa agaggtcgcg tcatcgcggt tcacctccct
      601 cgcgagcatc cgctcttccc agcgtcgtgt gaagggtcgt aggtccatac ccaccctcac
      661 cggagggccg tcgtcccgcc cggggagggt cgcccgaccg tggcgcccct ggagccgtcc
      721 cgccaccctg agcgaggagg ggggtgttct ccgtgacgct cgaagcacgg ctctccggcg
      781 gagggggggc ggtgtgggca tgatcggcga gcggttggac gtctgggacc tcctggcccc
      841 ggggatgctg atcagcaacc tgccctggat gctggtgctg gcctggttct cacgtcggct
      901 cctgggcctg aggcgacgct ccctgtggtc ctccgctgcg gccggcttga tcggctggct
      961 gatcggcagc ggcatcgccc tgctctcgat ccgcggcgac gtccagggcc actacgatgc
     1021 ggtttggcgg atcggcgtgg tgctgtcggt cctgttcacg atgctggcca ccgtcggcgt
     1081 ggagctgctg gcgcgccacc gggaggaggc cttctccttg ccgggcacca ccatccctca
     1141 cccggtccga agcgttcggg cgctcctggc tcgggtcggc cggtacatcc aggtcctcag
     1201 gatcgcggcc cgccatggcc tgcgcatgcg gccgggacat ccccccgacc gtctgccggc
     1261 ccgcgaggtg cgggccgcgc tcgaggagtg cggagggatg ttcgtgaagc tcggccagat
     1321 gctcgcgacc aggccggacg tcgtgggtcc gagcctggcg gaggagctca ccggactgca
     1381 ggaggacgcg ccgcccgctc cgaccgaaga cgtcgatcgg ctccttcagg aggagttcgg
     1441 tcatcccgtg ggcgaggtgt tcgagcgctt cgacgccgag cccatcgccg cggcctcgat
     1501 cgctcaggcg catagggccc ggctcggctc gggtgaggag gtcatcgtca aggtgcagcg
     1561 tccgggggtg gcgagcctgg tggagcgcga cctgtcgatc ctgctccgcc tgacccgccg
     1621 tgcggagcga cgggcgcgct gggccgagag ctacggggtg gcgaaggtgg ccgaagagtt
     1681 cgccgctggc gtgatcaagg agctggactt cacgatcgag gcccggagcg tcgaggagat
     1741 cggcgcggcg ctcggtgaca cgcccgagat ccgggttcct cacgtcttca cagagctctc
     1801 gacaccgcgg gtgctggtcc tcgagttcct ccagggggtc agcgtgggcc agcgtgagcg
     1861 cgtgcgtgag ctcggggtgg accacgagaa gctggccgat gtgctcctgc gatcgacgat
     1921 ctcgctggtg gtcgggggcg agcggttcca tgccgaccct catcccggca acgtgatggt
     1981 gctggccgac ggtcggctgg ggctgctgga cttcggctcg accggtcggc tggacgccat
     2041 cgaacgcgcg tcggtggtgg acatgctcac ggcgatcgca cagaacgacc ccgagctgct
     2101 ccgagacgcg gtcctggcca tcgccgtggt ccgccgtcgg atcgaggaac gcgccctgga
     2161 ccgggcgctc gccggcttca tggcccgcca cctcggaagg ggtgcggcgc cgagcgtggc
     2221 catgctccag gagcttgccc agatgttcct ggcgatgggg atcgcgctgc cctcgaccac
     2281 cacgatgttg ttccggatgc tggtcacgct cgaggggacg ctcacgaccc tgtgtcctgg
     2341 ctacgaactg atcggggccg ccagggatct cgcgccggag ctgatccgcg agcgactggc
     2401 tccctcgagg tggcaggagc aggcccggga ggagctcatg ggtttgatgc cggtcctgcg
     2461 acgggctccg cggcacctcg accgggtggc cacgatcctc gagcggggcg agctggccac
     2521 gagggtgagc ctgttcgcca ccgaggacga ccaacgcgtc ctccgcgctc tggtcaaccg
     2581 gaccctcctg gtcctgaccg gcggtgccgt gggggtggtg gcggttggct tgctggccgt
     2641 tcccggaggg ccgaccgtct cgggcgtcgt ctccctcttc gacgtcctcg gctacatggg
     2701 gctgttcctg tccgcggtgc tcctgctccg ggtcacggtc gccgccctga tcgaaggggg
     2761 atgaccgtca accgctccgc atcgcgggcg atcctcacgc cgcagcgact cggttcgtcg
     2821 gccccggggg ccagcatcgg ggggtcggcc ggtcctcttc caggggggcc ccacgcctct
     2881 tcgcgtgggc caccgcatgg gccagagtgg cctcgctgct ccggacgagt ctccacgctg
     2941 cggacggaag ggaggccgtg atgcgcctgt catggagaga cggactcgcc accctgctcg
     3001 tcggtgcggg agcgatcctc tacgccctgt ggctggctga tgcggaagtg gccgggttct
     3061 cgggtgtgag acccctggcc gggctcgtgc tcgggctggg cttggccgcc tcggtgaccg
     3121 cggtggtcta cggcgtaggt gccgggctcc tgcgggcgtc caaggtgtac ctgacggtcg
     3181 catccgtgat cggtctgacg gcgctggtgg ccgggacggt ggccctggtc ggggcgagcg
     3241 agacgatgct cgcggcgctg gtggcctcca cggtggcgct gtgggccatc tcgacggcgc
     3301 ggcacggcct gatcgccgag cacgccggag gccgggtcgc gcccacggct gccgggggcg
     3361 tcggagcccg agccccgagg ggagcccatg gctgagggcc ggaccggtgc agagcgccag
     3421 gagaggagga agcgatgagg agaggctgga tcgtggccgg cgtcgtgctg gcagccgcgt
     3481 acctggcccg ccggctcgcc gcccgctcgg gagccttgag ctgggagcag atgatcgagc
     3541 ggatgccgga caccgcccca ccgaaatggg tgttcaacaa catccaggca atccgcgaga
     3601 acaccgagca gatcctgggg cgactgagga gcgaaccggc cacggctccg tcatgaggga
     3661 agccttggcc atcggcgcgg cttcgcttgt cgcgctatgg ggtgtcgcac acgcgatccc
     3721 cacgcgcgcg gtggtggccg gcttcggccc gatctcgatc gacaaccggc ggatcatcct
     3781 gcaggagtgg ctggtcgagg ccttcgccat gtggggcatc gccgcgctgg tgatcggcgt
     3841 caccgtgttg gacgcggacg gcgaggcgac cgcctgggtc tgccggggat ccgccgccgc
     3901 gctcgtcgcg atcggtgttc tgaccgcgtt caccgggggc aggacgcaag tggtctggtt
     3961 caagatctgc actgtcctgc tgaccaccac ggccgcgctc ctcctggctg cgagcttcct
     4021 ctgagccaac ggggtgagcg tggatggcgc atctcccggc ctacgtcgat gccgtgcccg
     4081 gccggccgtt cccgctcgtc ccgacgttgc aggagctgcc ggcccgaggc catcgggtgt
     4141 ccgtgcgttg cggcgtccgg gaggtcgagc tgctccgatc ggtcgggtcc cggtcgcccc
     4201 aagccgagct cgtcgaagct gacgcgaccg cttcggggac gctgggcgac ctgaccaccc
     4261 atcttcctcc tgtggcgatc tggccgcacc aggtgtatcc cttccctcca ccccttcgtc
     4321 ctgcacggtg gatggatcag aagggaggtg actcagatgt tccgccgcaa cgagggggcg
     4381 ctggatcggg ccctcaggct ggcggccggg gaggagcacc ccgcgccgtc gctgtaggag
     4441 ggaggaggtc ctgggatgcc cgaggtggag gtcgatcgga acgaggacgt ggcgtgggca
     4501 tgcgggtgcg gcccggccat gcgccagatg gcggcggcct gctgcggcga gccggagccc
     4561 gagagcaccg gctcggaaga acaggagcag acgccacgcg tgcaaggagg tgcgtgagca
     4621 tgtggtggtg gggtcccgga tcctggtggc cctggatcgg gctgatgatt gggttcatgc
     4681 tgctctgcgg gatcttcatg gtccggatgg gcggcaggcg cggcatgggc ggcatgtgcg
     4741 gatggtggcg cgggcgcgga taccacgacc agacggacgc ggccgtcgcg cagcggtcga
     4801 gcgatcacga cgcgctggtt cgccccagcg cccgggggat cctcgaggag cgtttcgccc
     4861 ggggcgagat ctccgaggag gagttcgaag gtcgcaagca ggtcctggct cggaacccgt
     4921 cgtgacagct gtgcgcgatg gcaacggagg ccgcgggagc caacggttcc ggcggtctcc
     4981 gcgtgcatcg ctcggcgcca cgctgggagg ggccatcccc agctcacgcg gtgctggagg
     5041 atctcgacgc acgggacggt ctgtctcaga gagacatgac catcggccct atccgcctgg
     5101 gaccatggtc cctatcggcg ggagaggcct aggtctcaca tgatccgatg agccggtcat
     5161 cggggcctgg tgcatcgctc gaaaaggccc cggctcgccc cgctcgggtt tcctccggtg
     5221 aactcttcgc ctcctatcag gtcaggatcc gccggtacat ctcgagcatg gtgcacgacc
     5281 cggttgaggc cgatgacctc acccaggacg tgttcctgca ggtccaccgg aagctggact
     5341 cggtacgcga tcccgatgcg gtggtgtcct ggctgtaccg gatcgcgacg cacctgtgct
     5401 acgaccggtt ccgcaggtcc tcccgcgagc cgaggaccga cccgctcgac gtgggcggct
     5461 cgggcggggg gaggggtctc ggggacatcg ccgatcagtt gaggctggat cgggtgctcg
     5521 agcagaggga gatgagcgcc tgcgtcagga gctacatcga cgggctgtcc acccagtacc
     5581 ggcaggtgat cctcctgcac gacctggagg aggtgagcaa cccacagatc gcccagatgc
     5641 tcggggcctc gctcgacacg atcaagatcc gcctgcatcg cgcccgccgc aagctccagg
     5701 tggccctggg ggagcactgc ctgctgtcgc tcgatgagca aggggtcctc gtctgtgagc
     5761 cggccgaggt cacgaccgca gcaacctcgg ggagtgtgcg cgggagcccc tagtcgtcgc
     5821 gctccgccgg ctcctcggcc ggtaacgccc aaccctgcag ggctgccctt cggaaggcga
     5881 gcggtgagca tcttcgtcga agcggcggtg tggatccctt gccgcccggc ccgtgcacct
     5941 gaccggactc gcgtgtgtgc ttcgcccctt ccccggatca gctcggggcg tggagcgcca
     6001 cgaccctggc cgaggacgtc ggtgaggttc ccggatctgc cgagcccgcg cttctctcgc
     6061 cgatcaagag gaacgtccgt aggcgaccct tgcctttgac ctcgatgatt ccccgct
//
LOCUS       DASQQQ010000125         4159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_309943, whole genome shotgun sequence.
ACCESSION   DASQQQ010000125 DASQQQ010000000
VERSION     DASQQQ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309943"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="VE646_05275"
     CDS             complement(<1..1079)
                     /locus_tag="VE646_05275"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HZD79432.1"
                     /translation="MDLSISLDNGRAREPAVAGAKAAGLALARAAGLPVLPGWIVPTH
                     LAAPAADAGLEALRRTGAPSATRAVHAVPVGEPLLRDLERIARRIGRDAIVRSSAPQE
                     GDPAWAGAFASYVEVTGADLATAVRGCWASAFARDALARCRALGVDPRAVRVAVLIQP
                     WTPFEAGGTARVDRHGRVAVSAAPGWPGALAAGRVGGWDLVVSRHNEVAAGGSRGTGV
                     DVWLVRDVAALARAAREATGHPVIEWGARGNELTLLQVRSTRVRRVGASDRATTRRRA
                     APRPLLGARGDLAERLAVLAARFGGPLGDRLVLPWALAADPLPAASDTAQGDPAAAVA
                     EARELSRRLTAAAWGLPPGAASAAAS"
     gene            complement(1083..2321)
                     /locus_tag="VE646_05280"
     CDS             complement(1083..2321)
                     /locus_tag="VE646_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HZD79433.1"
                     /translation="MKETFGYANVEWKQGIDWTAVREWRLRRAREAMKRHGLGAMLLM
                     YDENVRYVTSTLTPGWNRLKPGLRYAVLCEGKDPILYEQGDIGLHILKNSPWIPPANV
                     RHSFAWIKGAAGAASQMQVEKFARALLDDLEDAGVNDQPLGVDFIDINMMRAFERAGV
                     TWTDGMTPMMEARAVKSPGEQDGLRIVGSICDALHYEMSQFLRPGLTENQVAAFGFEY
                     LYSFPGVEDVEDVIVSSGPNAWPNWRNFSDRIIRPGELVIIDVAALTWNGFKSCMYRT
                     YCVGGKPTDEMRAYYDEALTWLRDSIDAVKPGVTTRDIASTWPSAMDAWGYEDEDQAA
                     ANLWGHGLGLAQYDQPVISRIWSLDHPVEIQPGMVFALETQHGKNLEFGVRLEEMLVV
                     TEHGHELITSFPIDEITVVD"
     gene            complement(2340..3557)
                     /locus_tag="VE646_05285"
     CDS             complement(2340..3557)
                     /locus_tag="VE646_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxyltransferase"
                     /protein_id="HZD79434.1"
                     /translation="MTGEPWHTDRWFTSPWNFLPEVRADLSLPETVQVHDVTLRDGEQ
                     QAGVVFTKDDKLRIAEALAEAGVPRIEAGLPAVSAADEAAVREIAGMGLPARVYAFSR
                     CMVDDVRRAADCGVAGLVMEIPCSHHLIEQAYRWPVQRAVDATVEATSLAHELGLLVS
                     FFPIDATRAGLTEYLRMIETVAKDGHVDAVGLVDTFGVLSPHGVRLFVRRSRELLGVP
                     LETHFHMDFGLGVANTLIAVAEGVEVIQTTVTGTGERAGNTPMEETVLALLTMYGIDT
                     GIKTERFFDLSKLVMELAGVSQPSNRPVVGERLFNVESGIITTWVRNVGEDLVEAFPY
                     RPELVGQREPQLVLGKGSGLDSVAAALERHGRTATPEQTEQILAEVKARSLAAKALLG
                     DEGFLEIVDQVTG"
     gene            3780..>4159
                     /locus_tag="VE646_05290"
     CDS             3780..>4159
                     /locus_tag="VE646_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZD79435.1"
                     /translation="MRIPPVTARDHLDDEGRAAFDRIVETRGEILRPFQTFLHSPAVA
                     RSVAELGHVVRFRSHLGDRDRELVTVATGRARACPFIWESHLDAARRAGLREESIQAL
                     VSGGEGLERRERLLTTFVSELCETG"
BASE COUNT          613 a         1509 c         1428 g          609 t
ORIGIN      
        1 gacgccgcgg cgctggcggc accgggcgga agtccccagg ccgcggcggt cagccgccgg
       61 ctcagctccc gggcctccgc gacggccgcc gccgggtcac cctgggcggt gtcggaggcc
      121 gccgggagcg ggtcggcggc gagggcccat gggagcacga ggcggtcgcc gagcgggcct
      181 ccgaagcggg ccgcgaggac ggcgagacgc tctgccaggt caccccgggc accgaggagg
      241 ggtcggggcg ccgctcgccg tctcgtcgtc gcccgatccg aagccccgac ccgccgcact
      301 ctggtggacc ggacctgcag cagggtgagc tcgttgcccc gggcccccca ctcgatcacc
      361 ggatggccgg ttgcctccct cgcggcgcgg gccagggccg cgacgtcccg aacgagccag
      421 acatcgacgc cggttcccct cgacccgccg gcggcgacct cgttgtgccg cgacacgacg
      481 aggtcccacc caccgacccg tccagccgcc agggccccgg gccacccggg ggctgcactc
      541 accgcgaccc gaccgtggcg gtcgacgcgc gccgtcccgc ccgcctcgaa cggggtccag
      601 ggctggatca ggaccgccac ccgaacagcg cgggggtcga ccccgagcgc ccggcacctg
      661 gccagggcgt cccgggcgaa cgccgaggcc cagcaacccc gtacggcggt cgccaggtcc
      721 gcccccgtga cctccacgta ggaggcgaag gctcccgccc acgccgggtc gccctcctgt
      781 ggcgccgagg accgcacgat cgcgtcccgg ccgatccgcc tcgcgatccg ctccaggtcg
      841 cggagcaggg gttcgccgac tgggacggcg tgaaccgccc gggtcgctga cggggcgccg
      901 gtgcgccgca gcgcctccag ccccgcatct gcggccggcg cggccaggtg ggtgggaacg
      961 atccagccag gcaggacggg caggcctgcc gcccgggcca gggccagccc ggccgccttg
     1021 gccccggcta ccgccggctc gcgggcgcgg ccgttgtcga gggagatcga gaggtccacc
     1081 gatcagtcga ccaccgtgat ctcgtcgatg gggaacgagg tgatgagctc gtgcccgtgc
     1141 tcggtgacca cgagcatctc ctccaggcgc acgccgaact cgaggttctt gccgtgttgg
     1201 gtctccaggg cgaacaccat gcccggctgg atctcgacgg ggtgatcgag cgaccagatc
     1261 cgcgagatga ccggctggtc gtattgggcc aggccgaggc cgtgccccca gaggttcgcc
     1321 gcggcctggt cctcgtcctc gtagccccag gcgtccatgg ccgagggcca tgtcgacgcg
     1381 atgtcgcgcg tggtcacgcc gggcttcacc gcatcgatcg agtcgcggag ccaggtgaga
     1441 gcctcgtcgt agtaggcccg catctcgtcg gtgggcttcc cacccacgca gtacgtgcgg
     1501 tacatgcagg acttgaagcc gttccaggtc aaggcggcca cgtcgatgat cacgagctcg
     1561 cccgggcgga tgatccgatc ggagaagttc cgccagttcg gccaggcgtt cggcccggag
     1621 gacacgatca cgtcctccac gtcctctacc ccagggaagc tgtacaggta ctcgaatccg
     1681 aatgccgcga cctggttttc ggtgaggccc ggcctgagga actgggacat ctcgtagtga
     1741 agggcgtcac agatggaccc cacgatgcgc aggccatcct gctcgcccgg gctcttgacc
     1801 gcccgggcct ccatcatcgg ggtcatcccg tcggtccagg tgacgcctgc ccgctcgaag
     1861 gcccgcatca tgttgatgtc aatgaagtcc acgccgagcg gctggtcgtt cacgcccgcg
     1921 tcctccaggt catccagcag ggccctggcg aacttctcga cctgcatctg cgaggcggcg
     1981 ccggctgcgc ccttgatcca ggcgaacgag tggcggacgt tcgccggtgg gatccacggc
     2041 gagttcttca ggatgtggag cccgatgtcg ccctgctcgt agaggatggg atccttcccc
     2101 tcgcacagca cggcatagcg gagccccggc ttcaggcggt tccacccggg cgtgagggtc
     2161 gaggtcacgt agcgtacgtt ctcgtcgtac atgagcaaca tcgcgcccag gccgtgacgc
     2221 ttcatggctt ctcgggctcg gcgcagccgc cactcccgga cggccgtcca gtcgatcccc
     2281 tgcttccact cgacgttcgc gtagccgaac gtctccttca cgtcagtcca cctccccggt
     2341 cagccggtta cctggtccac gatttccagg aatccctcgt cgcccagcag ggctttggcc
     2401 gcgagcgagc gcgccttgac ctcggcaagg atctgctcgg tctgctcggg ggtcgccgtg
     2461 cgcccgtgcc gctcgagcgc ggcggccacc gagtcgaggc ccgagccctt gcccaggacg
     2521 agctgcggtt cacgctgccc caccagctcg ggccggtacg ggaacgcctc gacgaggtcc
     2581 tcgccgacgt tgcgaaccca ggtcgtgatg atcccggact ccacgttgaa cagccgctct
     2641 ccgacaacgg gccggttcga gggctgggac accccggcca gctccatgac gagcttcgaa
     2701 agatcgaaga accgctcggt cttgatcccc gtgtcgatcc cgtacatcgt gaggagcgca
     2761 agaaccgtct cctccatggg cgtgttgccg gcccgctccc ccgtgccggt gaccgtggtc
     2821 tggatcacct cgacgccctc tgcgacggcg atgagggtgt tcgccacccc cagaccgaag
     2881 tccatgtgga agtgcgtctc caggggcacg ccgaggagtt cccggcttcg ccgcacgaac
     2941 agccggacgc cgtgggggga caggaccccg aacgtgtcga ccagaccgac cgcatcgacg
     3001 tggccgtcct tggccacggt ctcgatcatg cggaggtact cggtgagccc cgcccgggtg
     3061 gcgtcgatgg gaaagaacga aaccaggagc ccgagctcgt gggcgaggct cgtggcctcc
     3121 acggtcgcgt cgacggcccg ctggacgggc caccggtacg cctgctcgat gaggtggtgg
     3181 ctgcagggga tctccatcac caagcccgcc acaccgcagt ccgccgcccg cctcacgtcg
     3241 tcgaccatgc accgcgagaa ggcgtagacc ctcgcgggca gccccattcc ggcgatctcg
     3301 cggaccgcgg cctcgtccgc cgcggacacc gcaggcaggc cggcctcgat ccgggggacc
     3361 cccgcctcgg ccagggcctc ggcgatccgc agcttgtcgt ccttcgtgaa gaccacgccc
     3421 gcctgctgct cgccgtcgcg gagggtcacg tcgtggacct gcacggtctc cgggagtgac
     3481 aggtcggccc gcacctcggg gaggaagttc cacggactcg tgaaccatcg gtccgtatgc
     3541 cagggttcgc cggtcatcgt gcccctctcg cggacagcgc ttatgtatta cacacaatac
     3601 acaaatcgcg ggcaacggac gggtcccttg gaggtaagcg gtcggagcgg actgaccgcg
     3661 gtgggccacc gcgcccgggg agaggacggg agaccaccgc tcgctccgca ggccgggatg
     3721 cagtcgatcg ggtgagtgcc ccaggggcgc ccctgtatat agtgtgtaat ctaaggacaa
     3781 tgcggatccc accggtaacc gcgcgcgacc acctggacga cgaggggcgg gccgccttcg
     3841 accgcatcgt cgagactcgt ggcgagatcc tgcggccgtt ccagacattc ctgcattccc
     3901 ctgcagtggc gagatcggtg gccgagctgg gccacgtggt caggttccgg tcacacctgg
     3961 gcgaccggga ccgcgagctc gtcaccgtcg ccaccggacg ggcccgggcc tgccccttca
     4021 tctgggagtc gcacctcgac gcggcccggc gggctgggct ccgcgaagag tcgatccagg
     4081 ccctcgtttc cggcggcgag gggctcgagc ggcgggagcg gctcctgacg accttcgtct
     4141 cggagctctg cgagaccgg
//
LOCUS       DASQQQ010000126         2857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_204106, whole genome shotgun sequence.
ACCESSION   DASQQQ010000126 DASQQQ010000000
VERSION     DASQQQ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204106"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1525)
                     /locus_tag="VE646_05295"
     CDS             complement(<1..1525)
                     /locus_tag="VE646_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79436.1"
                     /translation="MGLASRALRIRPGEGRLVGLLIGLAFVALASLAIGQSGIEALFF
                     DRIGTRKLPLMYLLQGGTTFLAMLGLTGLLGRLGPRRTYVLAPLALAVVVLAERVLLL
                     SGGRWVYPALWLTVPIVVLAQGVVLWGTAGAVVDTRQAKRLFPVFAAGGIVGSVAGGL
                     ATRPLAGLLGTSNLTVVWAAGLAGTFLLCRIVLGGAGSQARRASALREMVGGFRSVRR
                     SALLRWMTAAAVLFSVLYYSLYLPYARVATDRFPDADRLAGFFGIFWAAVTGVAFLVS
                     MLVANRLFAWLGVAAMTIVLPLLYGGAFGILLVESGFVALVGMRFVTGVWLQGVAAPG
                     WETLVNVVPEDRRDQTRAFLNGGPTQAGTVIAGVVALVGQQALSPRQFALIGLAAAVL
                     TTIAAIGIRRSYAGALLDALRAGRPQVFELASERPGVIGDAQAVRALTEAAGSPDVRL
                     RRLAFDLLSEVAGGLPEWWLADEDPVVRLLAVRGLSEVPGGLRPLVEDPHPAVATAAA
                     "
     gene            complement(1546..2373)
                     /locus_tag="VE646_05300"
     CDS             complement(1546..2373)
                     /locus_tag="VE646_05300"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZD79437.1"
                     /translation="MTRYETPEQEWQAYLGGTHPHLQHRSPLRLVPSGPRCKLCGAPF
                     GAPGRLVLGRYGFAPWEKNPKICKRCFHGLRGAARACPAAGGKADQEVRGGEVEISML
                     FADVRGSSKLARSMSTMDFTKLMNRFYRVSTDILIDRDAIIEKFVGDEVVGLFVPFLA
                     GPRHAELALDAARALLEATGHGSEDGPWLPLGAGVHTGSAFVGIVGSEGSNDFTALGD
                     PVNLAAHLASQAAVGEVLITDATATGAELSPDGLERRHVSLKGYPLDVLVVPAGATG"
     gene            complement(2420..>2857)
                     /gene="dtd"
                     /locus_tag="VE646_05305"
     CDS             complement(2420..>2857)
                     /gene="dtd"
                     /locus_tag="VE646_05305"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015920266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HZD79438.1"
                     /translation="VLQRVSWARVSVDRRTVAEIGRGLVLLVGVGRDDGFGDYDRLAQ
                     KVVNLRVFADRGDRMDRSLREVGGEVLVVPNFTLYGELRRGRRPSWEGAAPPEVAEGR
                     VEAFARALEACGATVRRGAFGAHMRVELENDGPVTLVIDTASL"
BASE COUNT          452 a         1136 c          965 g          304 t
ORIGIN      
        1 cggccgcggc ggtggccacc gccgggtggg ggtcctccac gagcggccgg aggcccccgg
       61 gcacctcgga caggccgcgg acggccagca ggcgcacgac cgggtcctcg tccgcgagcc
      121 accactcggg caagcctccg gcgacctcgc tcagcaggtc gaacgccagg cggcgcaggc
      181 gcacgtcggg ggacccagcg gcctcggtga gcgcacggac cgcctgggcg tccccgatca
      241 ccccagggcg ctccgaggcg agctcgaaga cctgtggacg gccggcgcgg agcgcgtcca
      301 gcagcgcgcc ggcgtacgag cgtcggatgc cgatggccgc gatcgtggtg agcacggcgg
      361 cggccagccc gatcaacgcg aactgccgag ggctcagcgc ctgctgcccc accagggcga
      421 ccacgccggc gatcacggtc ccggcctggg tgggaccgcc gttcaggaag gcgcgggtct
      481 ggtcccggcg atcctcgggc accacgttca ccagcgtctc ccaccccggc gcggccaccc
      541 cctgcagcca cacccccgtc acgaaccgca tccccaccag ggccacgaac cccgactcca
      601 ccagcaggat cccgaacgcg ccgccgtaca gaaggggcag cacgatcgtc atggccgcca
      661 ccccgagcca ggcgaacagc cggttcgcca cgagcatcga caccaggaac gccacgccgg
      721 tcaccgcggc ccagaagatc ccgaagaacc cggccagccg gtcggcgtcg gggaaccgat
      781 cggtggccac ccgcgcgtag ggcaggtaca gggagtagta caggaccgag aagaggaccg
      841 ccgccgcggt catccaccgc agcagggcgg accgacgcac ggaccggaac cccccgacca
      901 tctcccgcag cgccgaggct cgtcgggctt gggagcccgc gccgccgagg acgatccggc
      961 acagcaggaa cgtcccggcc agccccgccg cccacaccac cgtgaggttc gacgtgccca
     1021 ggagccccgc caggggccgg gtggccaggc ctccggccac cgaccccacg atgccgccgg
     1081 cggcgaagac cgggaacagg cgcttggcct gcctcgtgtc caccacggct ccggccgtcc
     1141 cccacaggac cacgccctgg gcgagcacga cgatcgggac cgtgagccat agagccgggt
     1201 acacccagcg gccgccggac agcaggagca cccgctccgc gagcacgacc acggccagag
     1261 ccagcggggc caggacgtag gtccgccgcg gaccgagccg gcccaacagg ccggtcaggc
     1321 caagcatcgc caggaaggtg gtgcccccct gcagcaggta catcaggggc agcttccggg
     1381 tgccgatccg gtcgaagaac agcgcctcga tcccgctctg gccgatggcc agcgaggcca
     1441 gcgccacgaa cgccaggccg atcagcaggc cgaccagccg gccttcgccc ggccggatcc
     1501 gcagtgcccg cgatgccagg cccatcggct ccctcccagg gccggctacc ccgtggcgcc
     1561 ggcgggcacc accaggacgt cgagcgggta gcccttcaac gacacgtgcc gtcgctccag
     1621 cccatcgggc gagagctctg ccccggtggc cgtcgcgtcg gtgatcagga cctcgcccac
     1681 ggcggcctgg gaggccagat gggccgcgag gttcaccgga tcccccagcg cggtgaagtc
     1741 gttggagccc tcggatccga cgatgcccac gaaggccgag cccgtgtgca cccccgcccc
     1801 cagggggagc caggggccgt cctcggagcc gtggcccgtg gcctccagca gggcccgggc
     1861 cgcgtcgagc gccagctcgg cgtgccgggg accggccagg aacgggacga agagccccac
     1921 cacctcgtct cccacgaact tctcgatgat ggcgtcccga tcgatcagga tgtcggtcga
     1981 gacccggtag aagcggttca tcagcttggt gaagtccatc gtggacatgc tccgggcgag
     2041 cttgctcgag cctcgcacgt ccgcgaagag catcgagatc tccacctcgc cgccccggac
     2101 ctcctggtcg gccttcccgc cggcggccgg acacgccctg gcggcgccgc gcagcccgtg
     2161 gaagcagcgc ttgcagatct tggggttctt ctcccagggc gcgaacccgt accgtcccag
     2221 caccagccgg cccggggcgc cgaacggcgc accgcagagc ttgcagcgag ggccggaggg
     2281 gaccagccgg agcgggctcc gatgctgcag gtgcggatgg gtcccgccca ggtaagcctg
     2341 ccactcttgc tcgggcgtct cgtacctcgt catggccgag ggcagcctag cgtcctcggc
     2401 cgggctggtg ccacaccgat cacagcgacg ccgtgtcgat caccagcgtg accgggccgt
     2461 cgttctcgag ctccacccgc atgtgcgccc cgaacgcccc ccgccgcacg gtcgcgccgc
     2521 aggcctcgag cgcgcgggcg aaggcctcca cccgtccctc ggccacctcg ggcggcgccg
     2581 ccccctccca ggagggtcgt cgcccccgac gtagctcccc gtagagggtg aagttcggca
     2641 ccaccaggac ctccccgccc acttcccgga gcgagcggtc catccggtcc ccgcggtcgg
     2701 cgaagacgcg caggttcacc accttctgcg ctaggcggtc gtagtccccg aacccgtcgt
     2761 cccgccccac ccccaccagc agcaccaggc cgcgcccgat ctccgccacg gttctccggt
     2821 ccacgctcac ccgggcccag gagacccgct gcaggac
//
LOCUS       DASQQQ010000127        20011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_147621, whole genome shotgun sequence.
ACCESSION   DASQQQ010000127 DASQQQ010000000
VERSION     DASQQQ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 20011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20011
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147621"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1376)
                     /locus_tag="VE646_05310"
     CDS             complement(144..1376)
                     /locus_tag="VE646_05310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZD79439.1"
                     /translation="MLTWEFPPRIVGGLSTHVAGLSRALAAMGHRIHVLTVGQPEAPT
                     EAWVDGVHVLRVPSPLPPTEPAGLIPWVVRFNTDLLDPGVDLLGTEGFDLIHAHDWLV
                     APAAIGLSGLCRRPLVATIHATERGRHQGYLPGPENEVIHRIERWLVEGADRLIACSR
                     FMAGQLWREFDTSSLKVDVIPNGTVVPHAPRRPVHGYGARRSPEERLILFVGRLEFEK
                     GVQTLLEALPAVHAQVPVRLLIAGTGTYEPCLRDRVRCSELSERVHFLGFCPQEQLHV
                     VRAAADLCVVPSLYEPYGMAAVEAMAAGVPCIAAHTGGLAEVISPGRTGLLFRPGDPA
                     TLAAQMIRVLSQPDLARTLAEGGRREAATRFGWGSVARATIAAYGRIVGGASAVLRQG
                     NGHAARGVVGGRLAEFTT"
     gene            complement(1647..1736)
                     /locus_tag="VE646_05315"
     tRNA            complement(1647..1736)
                     /locus_tag="VE646_05315"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1700..1702),aa:Ser,seq:cga)
     gene            1823..2215
                     /locus_tag="VE646_05320"
     CDS             1823..2215
                     /locus_tag="VE646_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZD79440.1"
                     /translation="MDEVRLRAIPLFAGLSRKEREQLARWADEVEVPAGTRLVDQGAF
                     PHEFFVISEGRAEVTIDGRPIAELGPGDFFGEIALLEGQRRTATVTSSTPLSAVVMHS
                     RDFGSMERSLPDVAARIRQEMTRRIGRD"
     gene            complement(2221..2673)
                     /gene="tadA"
                     /locus_tag="VE646_05325"
     CDS             complement(2221..2673)
                     /gene="tadA"
                     /locus_tag="VE646_05325"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HZD79441.1"
                     /translation="MRAGDEAMMRLALSEAELANGWGDVPVGAVVAREDEVLGRAGNQ
                     REHRNDPTAHAEVLALRQASEVLGTWRLEGCTMYVTLEPCAMCAGALVLARVRRLVFG
                     APDPRAGFAGSLGDLVRDPRLNHEVAVKAGVLGEACGDLLRAFFRDRR"
     gene            complement(2673..3017)
                     /locus_tag="VE646_05330"
     CDS             complement(2673..3017)
                     /locus_tag="VE646_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018793807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HZD79442.1"
                     /translation="MAGRARFEELVAEAVDSLPAWILERLENVEILVEDRPPTGEPNL
                     LGRYHGIPLVQRDGGYAGVLPDTITLYRSTIERTARDEDDLRRIVAHTVAHEIAHFFG
                     ISDDRLREIGRY"
     gene            3072..4115
                     /locus_tag="VE646_05335"
     CDS             3072..4115
                     /locus_tag="VE646_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79443.1"
                     /translation="MPAPDQLDLWQWIGVFGLSAAVLVVAGTSLAKWGDEIATRTGLS
                     GLLIGSLLAATATSLPELVIGVSASVSGAPALAVSDMFGSNMANMAILAVVDLMGRHR
                     VWPVVELGHARVASIAIALTAFAALGVIARPDLTVAGWVGADTLFIGVAYVSTVAWMR
                     RSRVSRVASLPGEGELPVPTGWSEPGRRGDVAPAALRFGAAAALILASAPFVTRSAEG
                     IAVTGGLSESFVGTAMLAVASSLPELVASLAAVRIGAHDLAVGNLFGSNAMNMAMLLI
                     FDLVYPKGPILAAVGGPGEATAAVASIALMGLTLAAIVHGEETRIRRLEPDAVVVLLA
                     YLAAMWAVWAASV"
     gene            complement(4139..5332)
                     /gene="nrfD"
                     /locus_tag="VE646_05340"
     CDS             complement(4139..5332)
                     /gene="nrfD"
                     /locus_tag="VE646_05340"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HZD79444.1"
                     /translation="MSAIAHVAPISAAARRARAWYALLVAGLAVGLGCFAYQWANGLT
                     VTGMSNTVSWGMYIISFMFLVGISAGGLIVVAGSELVRSKRFEPLGRLAIVVSATAIA
                     AAAISILPDLGRPQLVWKMLLQPNFTSPLVWDVAIITTYLAIAVADLWIMTRPEPRPG
                     ALRLVAFIALPAAVLVHSVTAWIFGLVVARPFWNTALMAPLFISSALVSGTALVILAA
                     HIAQRTTAFAPPDRVFPDLGRLMVWFIGADVFLLFAEVLTTITSRVPDHVEQLRVLLF
                     GRLAWVFWSEVALGAMVPFVIFAVPRLRASRPWLVTGASLALIGVFFKRVNILLSALF
                     EPLVGLAPGIPGGRPGQPFRPDQIYVPTWVEWGVLLGLASFVGLAISLGVRYLVLPRG
                     RHEPV"
     gene            complement(5329..6054)
                     /locus_tag="VE646_05345"
     CDS             complement(5329..6054)
                     /locus_tag="VE646_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZD79445.1"
                     /translation="MPEPATGPKLSPVEALPEASRRYGEVGAHSAEPRWGMAVDTERC
                     IGCWSCAVICKSENDVPLGMWWNRILTAGGDLDTPEVDEHGDLSMHWVPLACQHCGDP
                     PCEKVCPTSATYVDDRGTVLVNQADCIGCRYCMAACPYGVRVFNWGTPDRATDFDTGM
                     VQARPVGSVEKCTFCIHRLEEGQVPSCVWSCPARARIFGDLNDPDGQLVRTIEDRRGT
                     RLLEDKGTQPKVFYLEQRRRRSL"
     gene            complement(6047..8551)
                     /locus_tag="VE646_05350"
     CDS             complement(6047..8551)
                     /locus_tag="VE646_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZD79446.1"
                     /translation="MSVTRRAFLGGMGAAAGAAALGGAIAVGRSGIGAEPPASLLVDR
                     VVRTTCSPNCTGACGQLAFVRDGKIVKIQQAADYPDEAYNPRGCMKGLSFHNQIYGPD
                     RVMTPLIRTGERGSGRFRRATWGEALDHVAAELKRIGEAYGYDSIHVFGQVAGTGFVQ
                     KGVNYRAAGVLGMSHGTSFDYNGDLPMAMPITFGVQNADHEAKDWANARFLLVVGANP
                     LETRIPDAHFIFDAVEKGARLVVVDPVFSPTASKADTWVQLVPGTDAAFALGLVNQVL
                     AEGLEDRRFLITYTDAPLLVRSDTGKRLREADLAKGGATDRFLVWDRHRQMLATVGTD
                     RLGMPRGVDPALEGTFRVRMADGRTVEVTPGWALVAAEVSTWTPERASRVSGVPAELI
                     AKIARAFATEAPAAILMGGGANHWYHGDLTGRAYALLSVVTGNVGRSGGGFSVYVGQY
                     KVRGNVAPWWFPDDLHPPVIPTIYLVRGKTETMNPNTPYPRNGFKGLFVTFSNFFLQS
                     PDVNQLLGRLEELELVVVVDPQMTDTAAYADVVLPATTWYEKKDLVVSPLHPFIQVQQ
                     PAIEPLGESRSETWIWSELVKRIDRQRWARYFRGLTEDDAIDMILAGGSSTEGITREQ
                     LEQGPVRLNVPDPDIAFREQIEDLVPFPPQSLPVPLEVTAAFLPTRRIELYKEEDRFL
                     ELGEHVVTYKDPYDDGVHDPATYPLRLLSPHSKWRIHSTYSDNPWLAEIHGGEPGVLM
                     HPDDAAGRGIAENDPIEVFNTRGSLRARAHVSRAARPGVISLPEGWWARYYLAGKGVN
                     ELTSSAVNPIHEIYSVANIWAPSTGWKDCRCEVRRA"
     gene            complement(8548..8769)
                     /locus_tag="VE646_05355"
     CDS             complement(8548..8769)
                     /locus_tag="VE646_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79447.1"
                     /translation="MADRASRRRFLMGLLAVPAAAVGARALGALAATAFASRPSARGS
                     STTRCAQCGATAHAMLDPSCPDAPRVIVR"
     gene            complement(8932..9960)
                     /gene="speB"
                     /locus_tag="VE646_05360"
     CDS             complement(8932..9960)
                     /gene="speB"
                     /locus_tag="VE646_05360"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HZD79448.1"
                     /translation="MADRDFRLQRSELRSEDEFDEFAWDDLPYHTGWRGSFHKLPWAT
                     TEDEIRAAGADVAIVGAPLDDGTSARPGARFGPRAIREAPYHWGTTDAWSIQLEVEPF
                     RALTVVDAGDAPVVPSRLARGLRVIHEKVFRVAGTGAVPIVLGGDHSITYPSAAAVAR
                     HVHPRSVGIVHFDAHADTGNDVWGSLVSHGTPMRRLIEEGWVHGRNFVQIGLRGYWPE
                     KDTFDWMREHQLRWHTMVEIEERGAEAVIADAIVEALDGPECIYVSVDIDVVDPGTAP
                     GTGTPEPGGILPRELLRAIRQIVGRVDLVGMDVVEVSPPYDQSEVTAMLAHRAVLEAI
                     SALALKRS"
     gene            complement(10042..11064)
                     /gene="hypE"
                     /locus_tag="VE646_05365"
     CDS             complement(10042..11064)
                     /gene="hypE"
                     /locus_tag="VE646_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HZD79449.1"
                     /translation="MPLPPGDAVLLGHGSGGRLTAELIRDLFVPLFAGSDGSELSRLG
                     DAAVVEVNGTRLAFTTDGFVVTPKFFPGSDIGALAVNGTVNDLAMMGATPLYLSAALI
                     LEEGLPMDELGAIVASMAEACRRADVALVTGDTKVVERGSADGLFVTTTGIGVVPDGV
                     EVGPERARPGDVVIVTGPVGSHGVAVMSQRAGIEFEAELTSDTAPLTGLVRAMLAAGE
                     VRCLRDATRGGLASVLNELAEASQVSITVVEGDVPVLPPVRAACEMLGLDPLYVANEG
                     VAVAVVAPKAAEAALAAARSDPLGTHASVVGEVGVGPSRVRLRTGLGVTRPLLMLAGD
                     QLPRIC"
     gene            complement(11100..12206)
                     /gene="hypD"
                     /locus_tag="VE646_05370"
     CDS             complement(11100..12206)
                     /gene="hypD"
                     /locus_tag="VE646_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HZD79450.1"
                     /translation="MRFIDEYRDDRIARALLEQIGRTVTRPWTLMEVCGGQTHTLIRQ
                     GIDQVLPPQIELVHGPGCPVCVTPLALIDQAIEIAERPDAILCSFGDMLRVPGSSEDL
                     FMARAAGGDVRVVYSPLDAVEIARRNREKTVVFFAVGFETTAPANAMAVKQAKQLGVD
                     NFGLLVAHVLVPPAITAILVSPGNRVQAFLAPGHVCAVQGWREYEPLARDFRVPIVVT
                     GFEPVDLLEGILMVVRQLEAGRSEVENQYVRAVERDGNAAAMAVVDEVFETCDRAWRG
                     IGVIERSGLRLREPYRSYDAELRFPRQGRAASESEVCIAGEVLRGRAKPSHCPAFGAE
                     CTPEHPLGAPMVSSEGACAAYYLAGREREGAMTP"
     gene            complement(12203..12514)
                     /locus_tag="VE646_05375"
     CDS             complement(12203..12514)
                     /locus_tag="VE646_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020139559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HZD79451.1"
                     /translation="MCLGVPGKVVEVFEEDGIRMGTVDFGGIRRKACLEYAPEVELGG
                     FVVIHVGFAISVVDEEEAARAYELLAEMGNLEGIDLPQDPEGVRPEDVPDALGGRPEP
                     R"
     gene            complement(12514..14847)
                     /gene="hypF"
                     /locus_tag="VE646_05380"
     CDS             complement(12514..14847)
                     /gene="hypF"
                     /locus_tag="VE646_05380"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HZD79452.1"
                     /translation="MSVGSAMGSVIDTGRTRTALTPTTSARRFEVRGVVQGVGFRPFV
                     WRLASRLGLSGWVRNVSGVVQIHAEGSIQALDDLARAIAKEAPPLARVEDVRWTPAAV
                     LKIDAFTVEESRDGEGSDRLVSPDVATCSACLAELLDPDDRRYRYPFINCTDCGPRFT
                     IIESLPYDRERTSMRAFPLCEVCGREYRDPGDRRFHAEPVACSSCGPTLELLDGAGRR
                     LDGDPVAGGAALLRAGRIVALKGLGGFHLACDATDERTVTELRRRKARPDKPFAVMVA
                     DVDRALEVFSPSADELELLRSPKAPIVLVRDVGVLAPSVAPGHHRQGAMLPSTPLHHL
                     LLREVGRPLVMTSGNRSDEPICITNERTLHDLNGVADAFLVHDRDIVSRYDDSVTRVW
                     RGAPAVLRRARSYAPEPLELPLEVPAPILGTGALLHGAFCLASGRRAFLSQHVGDLDT
                     EEAMAAYGDALARYREVFGIEPQVVAHDLHPDFASTRFAVETGLPRVAVQHHHAHVAA
                     VMAEHGLSGTVIGVAFDGFGLGEDGTVWGGEFLVCDAARAERAGHLRPVPQPGGDAAV
                     RSPWRMAAAHLADAAALDEGLPLLGETAERDRGLNVVLAQVRSGPNAPLTSSAGRLFD
                     AVSCLAGVCRSATYEGQPAMLLEQIASGPAERSYPVAVQRVEGRLVLDTRPIVRGVVA
                     DLRAGRSAAEVASVFHATMATGIDRVCGEIRAGRGLSRVALSGGVFGNDLLLSDVVGR
                     LEADGFEVFVPREVPPGDGGIALGQVLVAAARMGGED"
     gene            complement(14888..15364)
                     /locus_tag="VE646_05385"
     CDS             complement(14888..15364)
                     /locus_tag="VE646_05385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HZD79453.1"
                     /translation="MARILVIGAGDTRRGDEGAGVVAAERLRDVAPVGIEVIAPGRIS
                     PETLAELEGVSHVLVLDAIDVGRRPGSIAAFETNSLSPCGSKVISAFGVADLFAAVGQ
                     TSDAPEEAIVLAIQPGATAPFTALSRPVRDAIPEMVQAALGVLRSWMAGGPMPLPT"
     gene            complement(15369..15779)
                     /locus_tag="VE646_05390"
     CDS             complement(15369..15779)
                     /locus_tag="VE646_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase/urease maturation nickel
                     metallochaperone HypA"
                     /protein_id="HZD79454.1"
                     /translation="MHDYHAVHVLVERLTDELARDDGAVAEVRIRAGVAFSPEALVQA
                     FEMLTPGTALEGSRLLVEGSTEETTCSACGHRWEATGDDLAGHWLVCPSCGALSEPEA
                     GAGLEVRTVVRSPDGGGEPPAGGEGSRGARSGGG"
     gene            complement(15737..16264)
                     /locus_tag="VE646_05395"
     CDS             complement(15737..16264)
                     /locus_tag="VE646_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HZD79455.1"
                     /translation="MRTLVLGLGNELAADDAVGLLVARAVRERLQGRADVVESSASGM
                     ALIEILAGYDRAVVVDAILTGRTAPGTVSEMGMVQVGRVVAPSTHQAGVPELAAVARR
                     LGLTFPTTTRVLAVEVEDPFTIGGAMSRSVAEAVAGMVDRVVDLVEGWALPDPSASGG
                     AACTTTTPSTSSSSA"
     gene            complement(16267..17727)
                     /locus_tag="VE646_05400"
     CDS             complement(16267..17727)
                     /locus_tag="VE646_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HZD79456.1"
                     /translation="MTEILIDPITRLEGHGRIHIFLNDDGAVDRAYLQIPELRGFEAF
                     AQGRRAEDMPQITSRICGVCPMAHHMAGTKTLDDLYHVQPPPAAVKVREMVYDAFMIE
                     DHALHFFYLGGPDFVVGPTAPKAERNILGVIGTVGLEIGKQVIAARKEIRGLMTEAAG
                     KAIHPVFGLPGGISKPISGDLRDRFVTGAAHAVELGKLALQLFRDVVLSNAAYVDLVT
                     SDAYTHRTYYMGLVDGQNRVNFYDGHLRVVDPEGKEFLSFDAKDYLEHVAEHVEPWSY
                     IKFSYLKDVGWKGFADGPGSGVFAVAPLARLNAAEGMSTPMAQEAYEELFSTLGGRPV
                     HHTLATHWARIVELINAAERMGELAADPEITDPEVRTIPTETPDEGIGVVEAPRGTLI
                     HHYRTDERGLITQANLIVATQSNAARIALSVDKAAKALIHGPEVDEGLLNEVEMAFRA
                     YDPCFGCATHALPGEMPLILEIRDRRGRLVREIRRD"
     gene            complement(17724..18713)
                     /locus_tag="VE646_05405"
     CDS             complement(17724..18713)
                     /locus_tag="VE646_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HZD79457.1"
                     /translation="MSKPKVAFYWCASCGGCEEAVVDLNEVVLDVVDAVDIVFWPVAL
                     DFKASDVEALADGELAASFINGAIRTSEQREMVELLRRKSGAVVAFGACAWLGGIPGL
                     ANLVTRESLLGYVYHGSPSTDGDGAEPREEVEVPEGILRLPRFDPDVRTLDQVIDVDY
                     YLPGCPPPADLIVQAVMALVEGRLPEKGTVLAPDVALCAECPRADTKPEDLKVERFHR
                     PYEIVADPKTCLLAQGLLCLGPATRSGCGAVCVAANMPCTGCMGPTSRVIDHGAKALS
                     AIASLMPSREREDVAASAEQIVDPVGTFYRYSLPASLLHRRVDRSPEPQEVTS"
     gene            complement(18710..19192)
                     /locus_tag="VE646_05410"
     CDS             complement(18710..19192)
                     /locus_tag="VE646_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HZD79458.1"
                     /translation="MDDGSFEPRIVAFFCTWCTYTAADLAGISRMQYAPNVRAIRLMC
                     SGRLDPQFILAALRGGADGVLIGGCHPGDCHYQEGNYNTLRRFELLTRILRDMGIEEE
                     RVRLEWISASEGDRVQKVCNEMTEQIRALGPLRLSTPPRVQPGESAAEGMELVGQVRP
                     "
     gene            complement(19197..>20011)
                     /locus_tag="VE646_05415"
     CDS             complement(19197..>20011)
                     /locus_tag="VE646_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZD79459.1"
                     /translation="IIHCVGSRDAKHNLWCSKVCCMYSLKLAHMVKERTGAEVYNFYI
                     DMRTPGKGYEEFYDKILREGVHMVRGRVAGVTDEAASPEEEGHLVIRAEDTLIGSVRR
                     IPVDMVVLAVGLEPQPDAADVRRLFNISCGTEGWFTERHPKLAPVSTFTDGIFLAGAC
                     QGPKDIPDTVAQAGAAAAEALAMVDRGFVTLEANTAHIDEDACSGCKTCVGLCPFGAI
                     AFEEERKKASVNEVICKGCGVCVGACPSGAAQQHLFTDAEILSEIEGVLAYV"
BASE COUNT         2988 a         7452 c         6822 g         2749 t
ORIGIN      
        1 gtgagcgtcc cggccaccct caagcgcctg ggttgcccgt actcgcagta gcactccgca
       61 tccggaaccc gaaggcacag catcaggacc tccttgccgt ctccacgtcc cactccctca
      121 ggtggtgagc tcaggtggtg agctcaggtg gtgaactcgg ccagcctccc tccgaccacc
      181 cctctcgccg cgtgtccgtt cccctggcgg agcacggccg acgccccgcc cacgatccgt
      241 ccgtaggccg cgatggtggc ccgcgccacc gatccccagc cgaaccgtgt tgcggcctcc
      301 cgccgcccgc cctcggccag ggtgcgcgcc aggtccggtt ggctgagcac acggatcatc
      361 tgagcggcca gcgtggctgg gtctccgggt cggaacagca gccccgttcg gccggggctg
      421 atgacctcag ccagaccccc ggtgtgggcg gcgatgcagg gcaccccggc ggccatggcc
      481 tccaccgccg ccatcccgta cggctcgtac agggatggca ccacgcacag atcggccgcg
      541 gccctcacca cgtgcaactg ctcctgggga cagaacccca gaaagtgcac ccgctccgac
      601 aactccgagc atcggacccg atcgcggagg cagggctcgt aggtgccggt gccggcgatg
      661 agcaggcgca ccggcacctg agcgtgcacg gcgggcagcg cctccaacag ggtctggacg
      721 cccttctcga actcgagccg gcccacgaac aagatgagcc gttcttcggg gctccgtctc
      781 gctccgtagc cgtgcaccgg cctgcggggc gcgtggggga ccacggtgcc gttcgggatg
      841 acgtccacct tcagcgagga ggtatcgaac tcgcgccaga gctgccccgc catgaagcgg
      901 gagcaggcga tcaagcgatc ggctccttcg accagccatc gctcgatccg atggatcacc
      961 tcgttctccg gcccggggag gtagccctgg tgacgaccgc gctcggtggc gtggatggtg
     1021 gccaccaagg ggcgacggca cagcccggac aggccgatcg cggccggggc gaccagccag
     1081 tcgtgcgcat ggatcaggtc gaacccctcc gtcccgagca gatccacccc gggatccagc
     1141 aggtccgtgt tgaagcgcac gacccacggg atcagccccg ccggctcggt ggggggaagg
     1201 ggggacggca cccggagcac gtggacccca tccacccacg cctccgtcgg cgcctccggc
     1261 tggcccacgg tcagcacgtg gatccggtgg cccatggccg ccagggcccg agacaggccg
     1321 gcgacgtggg tggagagccc gcccacgatc cgcgggggga actcccaggt cagcatcaga
     1381 accctcaggc ggcgaccact cgaaccgtcg acgctcggtg accgggatcc aacgcggccg
     1441 gccgtcctcg atgtcggcgc tcgacgcggg ccgatgggca tctgggcgat cagggaacgg
     1501 agtgtcgcca tacgccccac caccaagacg ttcgatggag aggagagggg cgttcactcg
     1561 agatcccgcc gatgatggcg gagccgccga ggacgggccc ctcccgatgc cgcgatgctc
     1621 ggtgaggccc accctcggtc agcgagcgga ggcggcgtga ttcgaacacg cgaggggcgg
     1681 tttagacccc taaccgcttt cgaggcggtc ccgttcgtcc gctccggcac gcctccgccg
     1741 gcgagtctag caatcccccg gtcgagggct cgccggtcgg gcccggggat ggcaggatgc
     1801 ccccaagggc aggaggggca gcatggacga ggtgcggctg agggcgatcc cgctgttcgc
     1861 ggggctgtca cgcaaggagc gggagcaact cgcccggtgg gcggacgagg tggaggtccc
     1921 cgccgggacc cggctggtcg atcagggggc cttcccccac gagttcttcg tgatctccga
     1981 gggtcgggcc gaggtcacga tagacggacg gcccatcgcc gagctgggcc cgggcgactt
     2041 cttcggcgag atcgccctgc tcgagggcca gcggcggacg gccaccgtca ccagttcgac
     2101 cccgctctcg gccgtggtca tgcactcgcg ggacttcggg tcgatggagc ggtcgctgcc
     2161 cgacgttgcc gccaggatcc ggcaagagat gacccgccgg atcggccgag actgaccggc
     2221 tcagcggcga tcccggaaga aggcccggag caggtcgccg caggcctcgc ccaggacccc
     2281 ggccttcacc gccacctcgt ggttcagccg gggatcgcgc accagatcgc cgagcgatcc
     2341 cgcgaagccg gccctggggt ccggggcgcc gaacaccagc cggcgcaccc gagccagcac
     2401 caaggccccc gcgcacatcg cgcacggctc gagggtcacg tacatcgtgc agccctccag
     2461 gcgccaggtc ccgagcacct cgctggcctg gcggagggcc aggacctccg cgtgagcggt
     2521 ggggtcgttc cggtgctcgc gctggttgcc ggctcggccc aggacctcgt cctccctggc
     2581 caccaccgcc cccacgggga cgtcgcccca cccgttcgcc agctcggcct cggacagggc
     2641 caggcgcatc atcgcctcgt cgcccgcccg catcagtacc gtccgatctc acgcaggcga
     2701 tcgtcggaga tcccgaagaa gtgcgcgatc tcgtgcgcca cggtgtgcgc gacgatccgc
     2761 cgaaggtcgt cctcgtcccg ggcggtccgc tcgatggtgg atcgatacag ggtgatcgtg
     2821 tccggcagca cgcccgcgta gcccccgtcc cgctggacca aggggatccc gtggtaacgg
     2881 cccagcaggt tcggctcgcc ggtgggcggc cgatcctcca ccaggatctc cacgttctcc
     2941 aggcgctcca ggatccatgc cgggagcgag tcgaccgcct cggccaccag ctcctcgaac
     3001 cgcgctcgtc ccgccatcca ccgtcagcgt agctcccggc cctccgcccg gccgcgtagg
     3061 ctgcggccag gatgccggcg ccggaccagc tggacctgtg gcagtggatc ggggtcttcg
     3121 gcctcagcgc agcggtgctg gtggtggccg ggacctcgct ggcgaagtgg ggcgacgaga
     3181 tcgccacccg caccggcctg tctgggctgt tgatcggctc gctgctggcc gccacggcca
     3241 cctcgttgcc cgagctggtg atcggtgtga gcgcctcggt gagcggagcg cccgcgttgg
     3301 ccgtctcgga catgttcggc agcaacatgg cgaacatggc gatcctggcc gtggtcgacc
     3361 tgatgggccg ccaccgcgta tggccggtgg tcgagctcgg tcacgcccgg gtggcctcga
     3421 tcgccatcgc cctcaccgcg ttcgcggcct tgggcgtgat cgcccggccg gacctgacgg
     3481 tggccggctg ggtgggggcc gacacgctgt tcatcggcgt cgcctacgtc tccaccgtgg
     3541 cctggatgcg tcgctcgcgc gtgtcgcgcg tggcctccct gccgggcgag ggggagctgc
     3601 cggtgcccac agggtggagc gagccgggcc gccggggcga cgtggcaccg gcggccctcc
     3661 ggttcggggc ggcggcggcg ctcatcctgg cgagcgcccc gttcgtcacc cgctccgcgg
     3721 agggcatcgc ggtgaccggt gggttgtcgg agagcttcgt cggcacggcg atgctggccg
     3781 tcgccagctc tctgccggag ctggtggcct ccctggccgc cgtccggatc ggggctcacg
     3841 acctggccgt gggcaacctc ttcggcagca acgcgatgaa catggccatg ctgttgatct
     3901 tcgacctggt ctacccgaag ggaccgatcc ttgccgccgt gggaggcccg ggcgaggcca
     3961 cggccgcggt cgcctccatc gccctcatgg gcctcacgtt ggcggcgatc gttcatgggg
     4021 aggagacgcg gatccggcgg ctggagccgg acgcggtggt ggtgctgctc gcctacctgg
     4081 ccgcgatgtg ggccgtctgg gcggcttcgg tgtgaggtcc tcgcccttcc ggggccggtc
     4141 agaccggctc gtgccgcccg cggggcagga ccaggtaccg gacccccagg gagatcgcca
     4201 gccccacgaa gctcgccagg ccgagcagca cgccccactc gacccacgtg ggcacgtaga
     4261 tctggtcggg tcggaacggc tgccccggcc ggccgccggg gatcccgggc gccagaccca
     4321 ccaggggctc gaagagggcc gacagcagga tgttcacgcg cttgaagaac acgccgatga
     4381 gcgcgagcga ggcgccggtc acgagccacg ggcggctggc ccgcagccgc gggacggcga
     4441 agatcacgaa cgggaccatc gcgccgagcg caacctcgct ccagaacacc cacgccagcc
     4501 ggccgaacag caggacgcgc agctgctcca cgtggtcggg gacccgggag gtgatcgtgg
     4561 tgaggacctc ggcgaacagc aggaagacgt cggccccgat gaaccagacc atcagccggc
     4621 ccagatccgg gaacacgcgg tcgggcgggg cgaaggcggt ggtccgttgc gcgatgtgcg
     4681 ccgcaaggat cacgagcgcg gtgcccgaga ccaaggccga ggagatgaac aggggagcca
     4741 tcagcgccgt gttccagaag ggccgcgcca cgaccaggcc gaagatccag gcggtcaccg
     4801 aatggaccag gacggcggcc gggagcgcga tgaaagccac caaccggaga gctccgggcc
     4861 tcggctcggg gcgggtcatg atccacaggt ccgccacggc gatcgccagg taggtggtga
     4921 tgatcgccac gtcccagacg aggggcgacg tgaagttcgg ctgcaggagc atcttccaca
     4981 ccagctgcgg acgccccagg tccggcagga tcgagatcgc ggcggcggcg atcgccgtgg
     5041 ccgagaccac gatggccagc cggcccagcg gctcgaaccg cttgctgcgc acgagctccg
     5101 agccggccac cacgatgagc cccccggccg agatcccgac caggaacatg aacgagatga
     5161 tgtacatgcc ccaggagacg gtgttcgaca tgccggtcac ggtcagcccg ttcgcccact
     5221 ggtaggcgaa gcaccccagg cccacggcca gaccggccac gagcagcgcg taccaggccc
     5281 gggcgcgacg ggcggccgcc gagatcggcg ccacgtgcgc gatcgcgctc acagggatcg
     5341 cctccgtcgc tgctccaggt agaagacctt gggctgggtg cccttgtcct cgagcagcct
     5401 ggttcctctg cggtcctcga tcgtccgcac cagctgcccg tcaggatcgt tgagatcccc
     5461 gaagatccgg gcccgggcgg ggcacgacca cacgcacgag gggacctggc cttcctcgag
     5521 ccggtggatg cagaaggtgc acttctccac gctgcccacc ggcctggcct ggaccatgcc
     5581 cgtatcgaag tccgtcgccc ggtccggcgt gccccagttg aagacgcgga ccccgtacgg
     5641 gcaggccgcc atgcagtacc ggcagccgat gcaatcggcc tggttgacca ggaccgtccc
     5701 tcggtcgtcc acgtacgtgg ccgaggtcgg gcagaccttc tcgcacgggg ggtcgccgca
     5761 gtgctggcag gccagcggaa cccagtgcat cgacaggtcg ccgtgctcgt ccacctcggg
     5821 cgtgtccagg tcgccgcccg cggtgaggat ccggttccac cacatgccga gcgggacgtc
     5881 gttctcggac ttgcagatca ccgcgcacga ccagcacccg atgcaccgct cggtgtccac
     5941 cgccatcccc caccggggct cggcgctgtg cgcccccacc tcaccgtagc gccgggaggc
     6001 ctcgggcagg gcctccaccg gtgagagctt cggaccggtc gcgggctcag gcacgtcgca
     6061 cctcgcagcg gcagtccttc cacccggtcg agggcgccca gatgttcgcc accgagtaga
     6121 tctcgtggat cgggttcacc gccgaagagg tgagctcgtt cacgcccttg ccggccaggt
     6181 agtaccgggc ccaccacccc tcgggcagcg agatcacgcc ggggcgggcg gcccggctca
     6241 cgtgggcccg ggcccgcagg ctgccgcggg tgttgaacac ctcgatcggg tcgttctcgg
     6301 cgatgccgcg cccggcggcg tcgtccggat gcatcagcac acccggctcg ccgccgtgga
     6361 tctcggccag ccaggggttg tccgaatagg tggagtggat ccgccatttc gagtgcggcg
     6421 aaagcagccg gagcgggtag gtggccggat catggacccc gtcgtcgtac gggtccttgt
     6481 aggtgaccac gtgctcgccg agctccagga accgatcctc ttccttgtag agctcgatcc
     6541 ggcgggtagg caggaaggcc gccgtgacct ccagcggcac cggcagcgac tgcggcggga
     6601 acggcaccag gtcctcgatc tgctcgcgga aggcgatgtc cgggtccggc acgttcagcc
     6661 ggacggggcc ctgctcgagc tgctcgcggg tgatgccctc ggtggagctc ccgccggcca
     6721 ggatcatgtc gatcgcgtcg tcctcggtca ggccgcggaa gtagcgagcc cacctctggc
     6781 ggtcgatgcg cttcaccagc tcgctccaga tccaggtctc cgagcgcgac tcgccgagcg
     6841 gctcgatggc cggttgctgg acctggatga acgggtggag cgggctgacc acgaggtcct
     6901 tcttctcgta ccaggtcgtg gccggcagca cgacgtccgc gtacgccgcg gtgtcggtca
     6961 tctgcgggtc gaccaccacg accagctcca gctcctccag ccgccccagc agctggttca
     7021 cgtccggcga ctggaggaag aagttcgaga acgtcacgaa cagccctttg aagccgttcc
     7081 gtgggtacgg ggtgttgggg ttcatggtct cggtcttgcc ccggaccagg tagatcgtgg
     7141 ggatcacggg cggatggaga tcgtctggga accaccacgg cgcgacgttg ccccggacct
     7201 tgtactggcc gacgtagacg ctgaacccgc cgcccgaacg tccgacgttg cccgtcacca
     7261 ccgacagcag cgcgtaggcg cgcccggtga ggtcgccgtg gtaccagtgg ttcgcgccgc
     7321 ctcccatcag gatcgccgcc ggggcctcgg tggcgaacgc ccgggcgatc ttcgcgatca
     7381 gctctgccgg gacgccgctc acccgcgagg cgcgctcggg cgtccaggtc gacacctcgg
     7441 cggcgacgag cgcccacccc ggggtcacct cgacggtacg gccgtcggcc atccgcaccc
     7501 ggaacgtgcc ttccagggcc ggatcgaccc ctcggggcat ccccagccga tcggtgccca
     7561 ccgtggccag catctggcgg tgccgatccc agaccaggaa gcgatcggtc gccccgccct
     7621 tcgccagatc ggcctcgcgg aggcgcttgc ccgtgtccga ccggaccagg aggggcgcat
     7681 cggtgtacgt gatcaggaac cgccggtcct ccaggccctc ggccaggacc tggttgacca
     7741 gaccgagggc gaacgcggcg tcggtgcccg ggacgagctg gacccaggtg tcggccttcg
     7801 acgccgtggg cgagaagacc gggtccacca ccaccagtcg ggcccctttc tccacggcgt
     7861 cgaagatgaa gtgagcgtcg gggatccggg tctcgagcgg gttcgccccc accacgagga
     7921 ggaacctcgc gttcgcccag tccttggcct cgtggtcggc gttctggacc ccgaaggtga
     7981 tgggcatggc catcggcagg tcgccgttgt agtcgaagct cgtgccgtgg ctcatgccga
     8041 gcacacccgc cgcccggtag ttgaccccct tctgcacgaa gccggttccg gcgacctgac
     8101 cgaagacgtg gatcgagtcg tagccgtagg cctccccgat ccgcttgagc tccgcggcca
     8161 cgtggtcgag cgcttccccc cacgtcgccc tgcggaagcg tcccgatcca cgctcgccgg
     8221 tccggatcag cggggtcatc acgcgatccg gcccgtagat ctggttgtgg aacgagagcc
     8281 ccttcatgca gccgcgcggg ttgtaggcct cgtccggata gtccgcggcc tgctggatct
     8341 tcacgatctt cccgtcccgc acgaaggcca gctggccgca cgcgccggtg cagttcggtg
     8401 agcaggtggt gcgcactacc ctgtccacca gcagcgatgc gggcggctcg gcgccgatac
     8461 cgcttcggcc caccgcgatc gccccaccca gggccgccgc gccggcggcc gcccccatcc
     8521 cacccaggaa cgcgcgccgg gtgacgctca ccgcacgatc accctcgggg cgtcggggca
     8581 ggaggggtcg agcatggcgt gagcggttgc gccgcactgc gcgcagcggg tggtgctgct
     8641 cccgcgcgcc gaagggcgcg atgcgaacgc cgtcgcggcg agcgcaccca gggcgcgggc
     8701 cccgacggcg gcggccggca ccgccagcag ccccatcagg aacctccgcc tcgacgctcg
     8761 gtccgccatc gcatcccctc ccgccggcac tgcgtcgcta ggatggcgat agtccccctg
     8821 ggcccgatgg acatgagacc catggcccta cccccgaggg acccgaagcc ctgtcccacg
     8881 tctcggcaga tggcgggacg tcgccgatgc gtgggagcga acgggcctgg gctacgaacg
     8941 cttcaaggcg agggccgaga tcgcctcgag gaccgcgcgg tgggcgagca tcgcggtgac
     9001 ctcgctctga tcgtagggag gggagacctc caccacatcc atcccgacca ggtccacacg
     9061 gcccacgatc tggcggatgg cccgcagcag ctcccggggc aggatcccac cgggctcggg
     9121 ggtcccggtg ccgggggcgg ttcccggatc gaccacgtcg atgtccacgg acacatagat
     9181 gcactcggga ccatccaggg cctcgacgat cgcgtcggcg atcaccgcct ccgctccgcg
     9241 ctcctcgatc tcgaccatcg tgtgccagcg cagctggtgc tcgcgcatcc agtcgaaggt
     9301 gtccttctcg ggccagtagc cgcgaaggcc gatctggacg aagttccggc cgtgcaccca
     9361 gccctcctcg atcagccgcc gcatcggcgt gccgtgcgag accagcgagc cccacacgtc
     9421 gttgccggtg tccgcgtggg catcgaagtg cacgatcccc accgatcgcg ggtgcacgtg
     9481 tcgggccacc gcggcggcgc tcgggtaggt gatggagtgg tcaccgccca gcacgatggg
     9541 cacggctccg gtgccggcca cccggaagac cttctcgtgg atcacgcgaa gcccccgggc
     9601 cagccgggag ggcaccaccg gggcatcccc ggcgtccacc acggtcagcg cgcggaacgg
     9661 ctccacctcg agctggatcg accaggcatc ggtcgtcccc cagtggtagg gcgcctcgcg
     9721 gatcgcacgg ggcccgaacc gcgctccggg ccgggcggac gttccgtcgt ccaagggggc
     9781 accgacgatg gccacatcgg cgcccgccgc gcggatctca tcctcggtcg tggcccaggg
     9841 cagcttgtgg aaggaccccc gccagcccgt gtggtacggc aggtcgtccc aggcgaactc
     9901 atcgaactcg tcctcggaac gaagctcgct gcgctgcagg cggaagtcgc ggtcggccac
     9961 ggtcgctcct cgttcgggat gggcagcctg cacgctaccc ccgcccccaa cgggccacaa
    10021 ccgccgggag ctcgctcgtg atcagcagat ccgggggagc tggtcgccgg ccagcatgag
    10081 gagcggtcgg gtgacgccca gccccgtcct cagccggacc ctggatgggc ccacacccac
    10141 ctccccgacg accgatgcgt gggtgcccag cggatccgag cgtgccgccg ccagggcagc
    10201 ctcggcggcc ttcggcgcca cgaccgcgac cgccacgccc tcattggcga cgtagagggg
    10261 gtcgagcccg agcatctcgc acgcggcacg caccggtggc aggaccggca cgtcgccctc
    10321 cacgaccgtg atcgagacct gcgaggcttc ggccagctcg ttcagcacgc tggccaggcc
    10381 gccgcgggtg gcatcccgca agcagcgcac ctcgcccgcg gccagcatcg ctcgcacgag
    10441 ccccgtcagc ggggccgtgt ccgaggtcag ctcggcctcg aactcgatcc cggcccgctg
    10501 cgacatcacc gccacgccgt gcgagcccac cggcccggtg acgatcacca catcacccgg
    10561 ccgggcgcgc tcgggcccca cctccacccc gtcgggcacg acgccgatgc ccgtggtggt
    10621 gacgaacaat ccgtcggcgc tcccccgctc gaccaccttc gtgtccccgg tcacgagcgc
    10681 cacatccgcg cgccggcagg cctcggccat ggaagcgacg atcgccccga gctcgtccat
    10741 cggcagcccc tcctccagga tcagggccgc ggacaggtac aggggcgtgg cccccatcat
    10801 cgccaggtcg ttcaccgtgc cgttcacggc gagcgcgccg atgtccgagc ccgggaagaa
    10861 cttgggcgtg accacgaagc catcggtggt gaacgcgaga cgggtgccgt tcacctccac
    10921 cacggccgca tcgccgagcc gcgagagctc ggaaccatcc gagcccgcga acagcggcac
    10981 gaacaggtcc cggatcagct cggcggtgag cctgccaccg cttccgtggc cgaggagcac
    11041 cgcgtcgccc ggaggcagtg gcacggggca ccgcatcgcg aacccccgcg cgcgatctct
    11101 cacggcgtca tcgccccctc gcgctcccgt cccgctaggt agtacgcggc gcaggcgccc
    11161 tcgctggata ccatcggcgc gccgagcggg tgctccgggg tgcactcggc accgaacgcc
    11221 gggcagtgcg acggcttcgc gcgcccccgg agcacctcgc cggcgatgca gacctccgat
    11281 tcggaggccg cacgtccctg ccgcgggaac cgcagctcgg cgtcgtagga gcggtagggc
    11341 tcgcggagcc ggagcccgct ccgctcgatc acgccgatcc cgcgccaggc ccggtcgcag
    11401 gtctcgaaca cctcatccac cacggccatg gcggccgcgt tcccgtcgcg ctcgaccgct
    11461 cgcacgtact ggttctccac ctccgagcga ccggcctcca gctggcggac caccatgagg
    11521 atgccctcga gcaggtctac cggctcgaac ccggtcacca cgatcggcac gcggaagtcg
    11581 cgggccagcg gctcgtactc tcgccagccc tgcaccgcgc acacgtggcc cggcgccagg
    11641 aacgcctgca cccggttgcc gggcgagacc aggatcgcgg tgatggccgg cggcaccagc
    11701 acgtgggcca ccagcagccc gaagttgtcc acgccgagct gcttggcctg cttgacggcc
    11761 atcgcgttcg ccggcgccgt ggtctcgaat cccacggcga agaacaccac ggtcttctcc
    11821 cggttccggc gggcgatctc gaccgcgtcc agtggcgagt acaccacccg cacgtcgcca
    11881 ccggcggccc gggccatgaa caggtcctcg ctcgaacccg gcacccggag catgtccccg
    11941 aacgagcaga ggatcgcgtc cggccgctcg gcgatctcga tggcctggtc gatcagcgcc
    12001 agcggggtca cgcagaccgg gcagccgggg ccgtgcacca gctcgatctg cggcgggagc
    12061 acctggtcga tcccctgccg gatcagcgtg tgggtctggc cgccgcacac ctccatcaac
    12121 gtccagggac gggtcacggt ccggccgatc tgctcgagca gggcccgggc gatccggtcg
    12181 tcgcggtact cgtcgatgaa cctcatcgcg gctcgggcct cccccccaag gcgtcgggca
    12241 cgtcctcggg ccggacgccc tcggggtcct ggggcagatc gatgccctcc aggttgccca
    12301 tctccgccag cagctcgtag gcgcgcgccg cctcctcctc gtccaccacc gagatggcga
    12361 accccacgtg gatcaccacg aagccgccga gctcgacctc gggcgcgtac tccaggcagg
    12421 ccttgcgccg gatccccccg aagtccacgg tgcccatgcg gatgccgtcc tcctcgaaga
    12481 cctccactac cttgcccggg acgccgagac acatcagtcc tcacctccca tgcgagccgc
    12541 ggccaccagc acctgaccga gggcgatccc gccgtccccc ggcggcacct cacgggggac
    12601 gaacacctcg aagccgtcgg cctccagccg tcccaccacg tccgagagca ggaggtcgtt
    12661 cccgaacacg ccaccggaca gggcgacgcg cgacaggccc ctaccggccc ggatctcgcc
    12721 gcacacccga tcgatcccgg tcgccatcgt ggcgtggaag acggaagcca cctcggcggc
    12781 gctccgaccg gcccgcaggt cggccaccac cccccgaacg atgggccggg tgtcgagcac
    12841 cagccgtccc tccacccgct gcaccgccac cgggtagctg cgctcggcgg gaccagacgc
    12901 gatctgctcg agcagcatgg cgggctgtcc ctcgtaggtc gccgaccggc agacaccggc
    12961 caggcacgac acggcatcga acaagcggcc ggcggagctg gtgaggggcg cgttcggtcc
    13021 cgagcgcacc tgggcgagga ccacgttcag gccccggtcc cgctcggcgg tctccccgag
    13081 cagggggagg ccctcatcga gcgccgcggc atcggccagg tgggccgcgg ccatgcgcca
    13141 cgggctgcgc acggccgcgt cccctcccgg ctgggggacg gggcgcaggt gccccgcgcg
    13201 ctccgcgcga gcggcatcac agaccaggaa ctccccgccc cacacggtcc cgtcctcgcc
    13261 cagcccgaac ccgtcgaagg ccacgccgat caccgttccg ctcagaccgt gctcggccat
    13321 caccgcggcc acgtgggcgt gatggtgctg cacggccacc cgaggcaggc ccgtctccac
    13381 ggcgaaccgg gtggacgcga agtcggggtg gaggtcgtgg gccacgacct ggggctcgat
    13441 cccgaacacc tcgcggtagc gagccaacgc gtccccgtac gccgccatcg cctcctcggt
    13501 gtccaggtcc ccgacgtgct gcgacaggaa ggcccggcgc ccggaggcca ggcagaacgc
    13561 gccgtgcagg agcgccccgg taccgaggat cggcgccggc acctccaggg gcagctcgag
    13621 cggctcgggc gcgtagctgc gggctcgccg caggaccgcg ggcgcccccc gccagacccg
    13681 ggtcaccgag tcgtcgtagc ggctcacgat gtcgcgatcg tggaccaaga aggcgtcggc
    13741 cacgccgttc aggtcgtgca gggtccgctc gttcgtgatg cagatcggct cgtcgctgcg
    13801 gttcccgctg gtcatgacca gcggacgtcc aacctcgcgg agcagcaggt ggtggagcgg
    13861 cgtggagggc agcatggccc cctggcggtg gtgcccgggg gccaccgagg gagccaagac
    13921 tcccacgtcg cggaccagca cgatgggggc cttcggcgag cggagcagct cgagctcgtc
    13981 ggccgagggg gagaagacct cgagcgcgcg atccacgtcc gccaccatga ccgcgaaggg
    14041 cttgtcggga cgggccttac gccggcggag ctcggtcacc gttcgctcgt cggtggcgtc
    14101 gcaggccagg tggaaccctc ccaggccctt cagcgccacg atccgacccg cgcgcaacag
    14161 ggcggccccc cccgccacgg gatccccgtc caggcggcga ccggcgccgt ccaggagctc
    14221 cagggtgggt ccgcacgagg agcaggccac gggctcggcg tggaagcgcc ggtcccccgg
    14281 gtcccggtac tcacggccgc acacctcgca cagcgggaag gcgcgcatgc tcgtgcgctc
    14341 ccggtcgtaa ggcagcgact cgatgatcgt gaaccgcgga ccgcaatcgg tgcagttgat
    14401 gaaggggtag cggtaccggc ggtcgtcggg atccaggagc tcggccaggc atgccgaaca
    14461 ggtggccaca tccggcgaga ccaggcggtc cgacccctcg ccgtctcggc tctcctccac
    14521 cgtgaacgca tcgatcttga gcacggcggc cggggtccac cgcacgtcct ccacccgagc
    14581 caggggcggc gcttccttcg cgatcgcgcg cgccaggtcg tccaatgcct ggatggatcc
    14641 ctccgcatgg atctgcacca cgccgctgac gttgcggacc catccggaca gccccaggcg
    14701 cgaggccagg cgccacacga acggccggaa gcccacgccc tggacgaccc cgcggacctc
    14761 gaaccgtcgg gccgaggtgg tgggggtgag cgccgttcgc gttcggccgg tgtcgatcac
    14821 ggatcccatc gcactgccca ccgacatcgt ccgcgatcgg cgagggggcg ccccgggtcc
    14881 accggcccta tgtcggcagg ggcatcggtc cccctgccat ccagctccgc aggaccccga
    14941 gcgccgcctg caccatctcc gggatcgcgt cacggacggg tcgggacagc gcggtgaagg
    15001 gcgccgttgc ccccggctgg atcgccagca cgatcgcctc ctcgggcgcg tcggaggtct
    15061 gccccaccgc ggcgaacaga tcggccaccc cgaacgcgct gatgaccttg gacccgcacg
    15121 gggacagcga gttcgtctcg aacgccgcga tcgagcccgg gcggcggccc acgtcgatcg
    15181 cgtccagcac caacacgtgg gagaccccct cgagctccgc cagggtctcc ggactgatcc
    15241 tgccgggggc gatgacctcg atgcccacgg gagccacgtc ccggagccgc tccgcggcga
    15301 ccacgcccgc cccctcgtcg cccctgcggg tgtcgccggc gccgatcacg aggatgcgag
    15361 ccaccccctc accccccgcc cgaccgcgct cccctcgacc cctcgcctcc cgccgggggc
    15421 tcccccccgc catccggcga acgcacgacg gtcctcacct cgagccccgc accggcctcc
    15481 ggctcggaca gcgcgccgca cgaagggcag acgagccagt gacccgcgag atcgtccccg
    15541 gtcgcctccc atcggtgccc gcacgcggag cacgtggtct cctcggtcga cccctcgacc
    15601 agcagccggg aaccctcgag cgccgtgccc ggggtcagca tctcgaacgc ctggaccaac
    15661 gcttcaggcg agaacgccac gcccgcgcgg atccggacct cggcgacggc gccgtcgtcc
    15721 cgggcgagct cgtccgtcag gcgctcgacg aggacgtgga cggcgtggta gtcgtgcacg
    15781 ctgctcctcc tgacgccgac ggatccggca acgcccagcc ctcgaccaga tccaccacac
    15841 gatcgaccat cccggcgacg gcctccgcca cggaccggct catggcgccg ccgatcgtga
    15901 agggatcctc cacctccacg gccaggaccc gcgtggtcgt cgggaaggtc aaccccagcc
    15961 gtcgcgccac ggcggcgagc tccggcacac cggcctgatg cgtgctcggg gccacgaccc
    16021 ggccgacctg gaccatgccc atctcgctga cggtacccgg ggcggtgcgg ccggtcagga
    16081 tcgcatccac gaccaccgcc cgatcgtagc cggcgaggat ctcgatcagc gccatcccgg
    16141 aggccgagct ctccaccacg tcggcccggc cctggaggcg ctcccgcacg gcgcgggcca
    16201 cgagcagacc cacggcgtcg tcggccgcca gctcgttgcc cagcccgagc acgagcgtgc
    16261 gcatgctcag tcccgccgga tctcccgcac gaggcggccg cgccggtccc tgatctccag
    16321 gatgaggggc atctctcccg gcagggcatg ggtggcgcac ccgaagcacg ggtcgtaggc
    16381 ccggaaggcc atctcgacct cgttcaggag cccctcgtcc acctccggac cgtggatcag
    16441 cgccttcgcg gccttgtcca cggacagcgc gatccgagcc gcgttgctct gggtggccac
    16501 gatcaggttg gcctgcgtga tcagcccccg ctcgtccgtg cggtagtggt ggatcagcgt
    16561 gccccggggg gcctccacga cgccgatgcc ctcgtcgggg gtctcggtcg ggatggtccg
    16621 gacctcgggg tcggtgatct cggggtcggc ggccagctcg cccatccgct cggcggcgtt
    16681 gatgagctcc acgatgcggg cccagtgcgt ggccagggtg tggtgcacgg gcctgccccc
    16741 gagcgtggag aagagctcct cgtaggcctc ctgtgccatc ggcgtggaca tcccctccgc
    16801 cgcgttcagc cgcgcgagcg gggccaccgc gaacaccccg ctgcccggcc catccgcgaa
    16861 gcccttccat cccacgtcct tcaggtacga gaacttgatg tagctccagg gctcgacgtg
    16921 ctcggcgacg tgctcgaggt agtccttggc gtcgaaggac aggaactcct tgccctccgg
    16981 gtccaccacg cgcaggtgcc cgtcgtagaa gttcacgcgg ttctgcccgt ccaccagacc
    17041 catgtagtag gtacggtgcg tgtacgcgtc cgaggtcacc aggtccacgt aggcggcgtt
    17101 ggacaggacc acgtcgcgga agagctggag ggcgagcttg ccgagctcga ccgcgtgggc
    17161 cgcgcccgtc acgaaccggt cccggaggtc cccggagatg ggcttggaga tgccgccggg
    17221 cagcccgaag accgggtgga tcgccttgcc ggccgcctcg gtcatgaggc cccggatctc
    17281 cttgcgggcg gcgatcacct gcttgccgat ctccagcccg accgtgccga tcaccccgag
    17341 gatgttccgc tcggccttcg gggcggtggg cccgaccacg aagtcgggtc cgcccaggta
    17401 gaagaagtgc agggcgtggt cctcgatcat gaaggcgtcg tagaccatct cgcggacctt
    17461 cacggccgcc gggggtggct gcacgtggta caggtcgtcc agggtcttcg ttccggccat
    17521 gtggtgagcc atcggacaga ccccgcagat gcgggaggtg atctgcggca tgtcctccgc
    17581 ccgacggccc tgcgcgaagg cctcgaaccc ccggagctcc gggatctgca ggtaggcccg
    17641 gtcgaccgct ccgtcgtcgt tcaggaagat gtggatccgc ccgtgtccct ccagacgggt
    17701 gatcggatcg atcaggatct ccgtcacgac gtcacctcct gtggctcggg gctgcgatcg
    17761 acccgtcggt gcagcaggga cgccggcagc gagtaccggt agaaggtccc caccggatcg
    17821 acgatctgct ccgcgctggc ggcgacgtcc tcgcgctccc ggctcggcat cagcgaggcg
    17881 atggccgata gggccttcgc cccgtggtcg atcacccggc tggtgggtcc catgcacccg
    17941 gtgcagggca tgttcgcggc cacgcacacg gctccgcatc ccgagcgcgt tgcgggcccc
    18001 aggcacagca ggccctgggc cagcagacag gtcttcggat cggccacgat ctcgtacggc
    18061 cggtggaacc gctcgacctt caggtcctcc ggcttggtgt cggcccgcgg gcactcggcg
    18121 cacagcgcca cgtcgggcgc cagcaccgtc cccttctcgg ggagccgacc ctccaccagg
    18181 gccatgaccg cctggacgat gagatcggcc ggcggcgggc accccggcag gtagtagtcg
    18241 acgtcgatca cctggtccag cgtccgaacg tccggatcga agcgcgggag ccggaggatg
    18301 ccctcgggca cctcgacctc ttcccgcggc tccgccccgt ccccgtcggt gctgggggac
    18361 ccgtggtaga cgtagccgag gagggactcc cgggtcacca ggttggccag gccggggatg
    18421 ccccctagcc acgcgcacgc cccgaaggcc acgaccgcac cggacttgcg ccgcaacagc
    18481 tccaccatct cccgctgctc cgacgtgcgg atcgcgccgt tgatgaacga ggccgcgagc
    18541 tccccatccg ccagggcctc gacatccgag gccttgaagt ccagggccac gggccagaac
    18601 acgatgtcca ccgcgtcgac gacgtcgagg accacctcgt tcaggtcgac cacggcctcc
    18661 tcgcatccac cgcacgaggc gcaccagtag aaggcgacct tcggcttgct catggcctca
    18721 cctgtcccac cagctccatc ccttccgcgg cggactcccc cggctgcaca cgcggcggcg
    18781 tgctcaagcg gagcggcccg agcgcgcgga tctgctcggt catctcgttg cacaccttct
    18841 gcacgcggtc gccctccgag gccgagatcc actcgagccg cacccgctcc tcctcgattc
    18901 ccatgtcccg caggatccgc gtgaggagct cgaaacgccg gagggtgttg tagttgccct
    18961 cctggtagtg gcagtcgccc ggatggcatc cgccgatcag cacgccgtcg gcccctccgc
    19021 gcagggcggc caggatgaac tgagggtcga gccggcccga gcacatcagc cggatcgcgc
    19081 ggacgttcgg cgcgtactgc atccgcgaga tcccggccag gtcggccgcc gtgtacgtgc
    19141 accaggtgca gaagaacgcc acgatccgag gctcgaagga gccgtcgtcc acgctctcag
    19201 acataggcca acaccccctc gatctcggag aggatctcgg catccgtgaa caggtgctgt
    19261 tgggcggcac ccgaagggca ggcgcccacg cagacgccgc accccttgca gatcacctcg
    19321 ttcaccgacg ccttcttgcg ttcctcctcg aacgcgatcg ccccgaacgg gcacaggccc
    19381 acgcatgtct tgcagccgga gcacgcatcc tcgtcgatgt gcgcggtgtt ggcctcgagg
    19441 gtcacgaacc cgcgatcgac catcgcgagc gcctcggccg ccgccgcacc ggcctgcgcg
    19501 accgtgtcgg ggatgtcctt cggcccctga caggcaccgg ccaggaagat cccgtcggtg
    19561 aaggtagaga cgggagcgag cttcgggtgc cgctcggtga accagccctc ggtgccgcag
    19621 gagatgttga acagccgtcg cacatcggcg gcgtcgggct gcggttccag gccgaccgcc
    19681 agcaccacca tgtcgaccgg gatccggcgg accgagccga tcagcgtgtc ctcggcccgg
    19741 atcaccagat gcccttcctc ctccggcgag gccgcctcgt cggtcacccc ggccacacgg
    19801 cctcggacca tgtgcacgcc ctcgcggagg atcttgtcgt agaactcctc gtagcccttc
    19861 ccgggcgtgc gcatgtcgat gtagaagttg tagacctcgg ccccggtgcg ctccttcacc
    19921 atgtgcgcga gcttcagcga gtacatgcag cacaccttgg agcaccacag gttgtgtttg
    19981 gcgtccctgg agccgacgca gtggatgatc c
//
LOCUS       DASQQQ010000128         6681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_4705, whole genome shotgun sequence.
ACCESSION   DASQQQ010000128 DASQQQ010000000
VERSION     DASQQQ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4705"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VE646_05420"
     CDS             <1..649
                     /locus_tag="VE646_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HZD79460.1"
                     /translation="SFANEIANLCELVGADPRRVLPAIGADHRVGEAFLGPGVGWGGS
                     CLPKDVAALIGMGQEYGYGAPLLRGARHVNLGQRRAVLRKLQRGLKVLKGRRVAVLGL
                     AFKAHTDDLRESPALELIEQLHDAGALVTAYDPAVKIAPAELDGIRMVPNAYDAADRA
                     DALVVVTDWPEFAELDLRQVAGRMDGDLLVDGRNLIDPDRAARAGLRLVGVGTGG"
     gene            812..1798
                     /locus_tag="VE646_05425"
     CDS             812..1798
                     /locus_tag="VE646_05425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZD79461.1"
                     /translation="MRRLRILTWHVHGGYLSALSHVPHEFYLPVKPGRPPGYAGRTGP
                     FRWPDTVHEVPADDVRRMRFDCVLYQSRDNYVWDGHEILSISQQRLPRIYLEHDPPRE
                     HPTDTRHVVQDPSVLVVHVTPFNALMWDTGDAPTRVIEHAVSIPPGVRYTGERQRGVV
                     VVNNLAARGRRLGLDVFDQVRREIPLDLVGMGSEELGGLGEVPYPELPIFEAPYRFLF
                     NPIRYTSLGLAVIESMHIGMPVVGLATTEMVTAIGNGVSGYVDTDPRRLVERMRHLLD
                     DPAEARRLGEGARRTAEARFGMDRFVHEWLDAFATVTGIPTPSSAWERRGAA"
     gene            1795..3711
                     /locus_tag="VE646_05430"
     CDS             1795..3711
                     /locus_tag="VE646_05430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZD79462.1"
                     /translation="MNRRVAVISAHASPLAILGGVDSGGQNVYVAQVARGLAARGYEV
                     DVFTRRDADLVPPVSEWVGGVRIVHVPAGPARHVPKEQLLAYAEEFTGHVVDAARRDG
                     YDLLHANFFISGLAAAEVKRRLGIPFVITFHALGRVRRLHQGADDGFPDERFAIEERL
                     VAEADHIVAECPQDEEDLVRLYNADPAKITIVPCGFDPAEFFPISKPLARSSLGFEPR
                     ERIVLQLGRMVPRKGVDTVIRGIARLNRDHGIPSRLVIVGGECEDPDPRATPEIGRLQ
                     RIARDEGVIDGVIFTGRRDRPELAKYYSAADVFITTPWYEPFGITPVEAMGCGTPVVG
                     SNVGGIKFTVRDAETGYLVPPNDPGVLAERLAHLYRHPKLLSLFGRQAVHRARDLFTW
                     DRVSEALVGLYEQVLAATRPAATDQPRRLQSVDRSFRSALEALEEGRRALPGPILRGA
                     DVLAACLRRGGKILICGNGGSAAQAQHFAAELVGRFEDDSRPPLPVISLTADTAVLTA
                     WSNDRSFSDVFARQVEALGQPGDVVVGISTSGRSPNVVRAFEAANRLGLTRVAILGGD
                     GGALRGRADVALVVPGPATPRIQEIHSVIVHVLCELIEQELSSGGHRRAAEMPLPDLS
                     SMWREPRPMAAEGA"
     gene            3708..4439
                     /locus_tag="VE646_05435"
     CDS             3708..4439
                     /locus_tag="VE646_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZD79463.1"
                     /translation="MTTQDLLGTVALVTGGGRGLGEAVCRTLARAGASVLVADLRKRE
                     ADVVAQDINEHEGEAVSVPLDVGDAQQAEDAIRTATDRFGRLDVLVNNAGIDVTQPVD
                     ELEVADWDRVLQTNLRGPFLLSRLALRVMKEQGSGSIVNVVSTAAKRAWANASVYHAS
                     KWGLMGFTQGLHVEGREHGVKVTALVAGGMRTPFLLERFPDIDPSTLQDPANVAEAVR
                     FVLTLPPETVIPELMVIPMRETSWP"
     gene            4490..5959
                     /gene="rfaE2"
                     /locus_tag="VE646_05440"
     CDS             4490..5959
                     /gene="rfaE2"
                     /locus_tag="VE646_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017318346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HZD79464.1"
                     /translation="MDRFAGLPVTVVGEAMLDSWMEGRTSRLCPEAPVPVVDVLGRTD
                     APGGPANVAVNARSLGAEVRFVSVIGDDPEGELARRCLQGRGLDDRDLLVQSGRRTLT
                     KSRITADRQILIRVDHGDTGPIDRFTERALLDRLTEAFVQSRAVIVSDYGYGLMTPTV
                     VAHLAALQRDRARILVLDAKDPTAYVDVGVTAVKPNFAQAMRLLGVPDGDTGQGFDRA
                     ASVIEVGDRLLELLGAEIVAVTLDAEGAVILERGRPAHRTYADPVRSSTTAGAGDTFS
                     AAFALALAVGAQTPTAAEIASAAASVVVAKPGTASCTPTELRQVLSPAAKRLDREGLG
                     ERMEAYRRQGKRIVFTNGCFDILHRGHIAYLNRAKELGDVLVIGVNDDAGVRRLKGPD
                     RPINALEDRIEVLAALSSTDHVVPFREETPAELIRAIRPDVFVKGGDYSVERLPEASL
                     VQALGGRVWILPFVEDRSTTGIIERIRRSGGGSLDRAPA"
     gene            5961..>6681
                     /locus_tag="VE646_05445"
     CDS             5961..>6681
                     /locus_tag="VE646_05445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZD79465.1"
                     /translation="MRGAGNWAGARNLLCIRLDALGDVLMTEPAIRALRGSLARSRIT
                     LLTSTSGAEACGMIAAIDDVIAYDPPWMRSGSGQRRREDRGMIDRLARHRFDGAVIFT
                     VYSQSALPAAYLTHLAGIPRSLAYGRENPYHLLTDHRAEDEPHRIVRHEVRRHLDLVA
                     SIGCSTPDERIHVRPSPRARRSIRERIGAMGLRRDEPWVVVHAGATAPSRRYPVERFA
                     EVVARLRRDLRWPVVLTGSETE"
BASE COUNT          924 a         2345 c         2468 g          944 t
ORIGIN      
        1 cagcttcgcg aacgagatcg ccaacctgtg cgagctggtg ggcgccgacc cccgtcgggt
       61 gctcccggcg atcggcgcgg accaccgcgt gggagaggcg ttcctcggtc cgggggtggg
      121 ctggggtggc tcgtgcttgc ccaaggacgt cgccgcgctg atcggcatgg gccaggagta
      181 cgggtacgga gcgcccctgc tgcggggagc ccgccacgtc aacctcggcc agcggagggc
      241 cgtgctgcgc aagctgcagc gcggcctgaa ggtcctgaag ggacggcggg tggccgtgct
      301 ggggctggcg ttcaaggccc acacggacga cctgcgggag tcccccgccc tcgagctgat
      361 cgagcagctc cacgacgcgg gcgctctggt caccgcctac gatcccgcgg tcaagatcgc
      421 tcccgcagag ctcgacggca tccggatggt gccgaacgcc tacgacgcgg cggaccgcgc
      481 cgacgccctc gtggtcgtca ccgactggcc cgagttcgcc gagctcgacc tgcgacaggt
      541 ggccggacgc atggacggcg atctgctggt ggacggccgg aacctgatcg accccgaccg
      601 cgcggcgcgg gccgggctgc gcctggtagg ggtgggcacc gggggctgat cgggggcccc
      661 gggctccacg tttatcggga agaggtgcgg gtagccagaa ccgtacgggc gagccgtgca
      721 gcactcaccc gctcgtcgac gcggcccgac gggacgagaa ggaggggttc gccatccgag
      781 tggcggtcac agatcgaggg ggacggcggt gatcagacgc cttcggatcc tcacctggca
      841 cgtccacggc ggctacctaa gcgcgctctc ccacgtgccc cacgagttct acctgccggt
      901 gaagccgggc cggccccccg ggtacgccgg ccggaccggg ccgttccggt ggccggacac
      961 ggtgcacgag gtgccggccg atgacgtccg gcggatgcgg ttcgactgcg tcctgtacca
     1021 gtcccgggac aactacgtgt gggacgggca cgagatcctg tccatctccc agcagcgcct
     1081 gccgcggatc tacctggagc acgaccctcc acgcgagcac cccaccgaca cccggcacgt
     1141 cgtccaggac ccctccgtgc tggtggtgca cgtgaccccc ttcaacgcgc tgatgtggga
     1201 cacgggcgat gcccccaccc gcgtgatcga gcacgccgta tcgatccccc ccggcgtccg
     1261 ctacacgggt gagcgccaga ggggggtggt ggtggtgaac aacctggcgg caagagggcg
     1321 gcggctgggg ctcgacgtct tcgaccaggt ccggcgcgag atccccctgg acctggtggg
     1381 gatgggctcg gaggagctcg gtgggctggg cgaggtcccc tacccggagc tcccgatctt
     1441 cgaggccccc taccgcttcc tgttcaatcc gatccgctac acgagcctgg gcttggcggt
     1501 gatcgaatcc atgcacatcg gcatgccggt ggtagggctg gccaccaccg agatggtgac
     1561 cgcgatcggc aacggcgtct ccggctacgt ggacacggat ccgcgccggc tcgtggagcg
     1621 gatgcggcac ctgctcgacg acccggccga ggcccgtcgc ctgggcgagg gggcgcgccg
     1681 gacggccgaa gcccgattcg gcatggaccg cttcgtgcac gagtggctgg acgcgttcgc
     1741 caccgtgacc gggatcccca cgccctcgtc ggcctgggag cgacgggggg cggcgtgaac
     1801 cgccgggtgg cggtcatcag cgcccatgca tccccgctcg cgatcctggg cggtgtggac
     1861 agcggcggtc agaacgtcta cgtggctcag gtcgcgcgcg ggctggccgc ccgcggctac
     1921 gaggtggacg tgttcacccg ccgggacgcc gacctggtgc cgccggtctc cgagtgggtg
     1981 ggcggggtcc ggatcgtgca cgtaccggcc ggccctgctc ggcacgtgcc caaggaacag
     2041 ctcctggcgt acgcggagga gttcaccggc cacgtggtcg acgccgctcg gcgcgacgga
     2101 tatgacctgc tccatgccaa cttcttcatc tccggcctgg ccgccgccga ggtcaagcgc
     2161 cggctcggca tccccttcgt catcacgttc cacgcgttgg gacgggtgcg tcggctgcac
     2221 cagggggccg acgacggctt ccccgacgag cgcttcgcga tcgaggagcg gctggtggcc
     2281 gaggctgacc acatcgtggc cgagtgcccg caggacgagg aggatctggt ccggctgtac
     2341 aacgcggatc cggccaagat cacgatcgtc ccctgcgggt tcgatcccgc cgagttcttc
     2401 cccatcagca agccgctcgc ccgttcgtcg ttgggcttcg agccccgcga gcggatcgtg
     2461 ctgcagctcg ggaggatggt gccccgcaag ggtgtggaca cggtgatccg gggcatcgcc
     2521 cgactgaacc gggaccacgg gatccccagc cgcctggtga tcgtgggcgg ggagtgcgag
     2581 gaccccgacc cgcgtgccac ccccgagatc gggcggctgc agcggatcgc ccgcgacgag
     2641 ggggtgatcg acggggtgat cttcaccggg cgccgggacc ggcccgagct ggccaagtac
     2701 tacagcgcgg cggatgtgtt catcaccact ccgtggtacg agccgttcgg gatcacgccg
     2761 gtggaggcga tgggctgcgg caccccggtg gtgggttcga acgtgggggg gatcaagttc
     2821 accgtgcgcg acgccgagac cggctacctg gtgcccccca acgaccccgg cgtcctggcc
     2881 gagcggctgg cccacctgta ccggcacccc aagctcctct cgctcttcgg tcgacaggcc
     2941 gtgcaccggg cccgggacct gttcacctgg gaccgggtgt ccgaggcgct ggtgggcctc
     3001 tacgagcagg tcctggcggc cactcgcccg gcggccacgg accagccccg caggctgcag
     3061 tccgtggacc ggagcttccg ctcggcgctc gaagcgctgg aggagggccg gcgcgcgttg
     3121 cccggtccga tcctgcgggg cgccgacgtc ctggccgcct gcctgcgccg gggcggcaag
     3181 atcctgatct gcggcaacgg gggcagcgcc gcacaggcgc agcacttcgc cgccgagctg
     3241 gtgggccggt tcgaggacga ctcccgaccg cccctgcccg tgatctcgct caccgccgac
     3301 accgcggtgc tcacggcctg gtccaacgat cgctccttct ccgatgtctt cgcccgccag
     3361 gtggaggcgc ttggacagcc cggggatgta gtggtcggca tcagcaccag cgggcggtcc
     3421 ccgaacgtgg tccgggcgtt cgaggcagcg aaccggctgg ggctcacccg cgtcgccatc
     3481 ctgggtggtg acgggggcgc gctgcgcggt cgggcggacg tggccctggt ggtcccgggg
     3541 cccgccaccc ccaggatcca ggagatccac tcggtgatcg tgcacgtcct ctgcgagctg
     3601 atcgagcagg agctgtcctc gggcggccac cggcgggcgg ccgagatgcc gctcccggac
     3661 ctgtcgtcga tgtggcgcga gccccgacca atggccgcgg agggggcatg accacccagg
     3721 atctgctggg cacggtggcg ctcgtcacgg gcggaggccg gggcctgggc gaggcggtgt
     3781 gccgcaccct ggcccgagcg ggcgccagcg tgctggtggc cgacctacgc aagcgcgagg
     3841 ccgacgtggt ggcccaggac atcaacgagc acgaagggga ggcggtgtcg gtcccgctgg
     3901 acgtgggcga cgctcagcag gccgaggacg cgatccgaac ggcaaccgat cggttcggcc
     3961 ggctggacgt cctggtgaac aacgccggca tcgacgtcac ccagcccgtg gacgagctcg
     4021 aggtcgccga ctgggaccgg gtcctgcaga cgaacctgcg agggccgttc ctgctctcgc
     4081 gactggccct tcgggtgatg aaggagcagg gctccggcag catcgtgaac gtggtgtcca
     4141 ccgcggccaa gcgcgcgtgg gccaacgcct ccgtgtacca cgcgagcaag tgggggctga
     4201 tggggttcac ccagggactg cacgtggagg ggcgcgagca cggcgtgaag gtcaccgccc
     4261 tggtggcggg gggcatgcgg acgccgttcc tgctggaacg cttcccggac atcgacccct
     4321 ccaccctgca ggacccggcc aacgtggcgg aggcggtccg cttcgtgctc accctccccc
     4381 ccgagaccgt gatcccggag ctgatggtga tcccgatgcg ggagacctcg tggccctgag
     4441 cgcgacggcc ccctcgccgt cgccgtctgg aggtgggacc gccgatctgg tggaccgatt
     4501 cgccggcctg ccggtcaccg tggtcggcga ggccatgctc gacagctgga tggagggtcg
     4561 gacctcccgg ctgtgcccgg aggcgccagt ccccgtggtc gacgtgctgg gccgcaccga
     4621 cgcaccgggc ggcccggcga acgtcgccgt gaacgcgcgc tcgctgggcg ccgaggtccg
     4681 gttcgtgtcg gtgatcggcg acgatccgga gggcgagctg gcgcgccggt gcctgcaggg
     4741 gcgcggcctc gacgaccgcg acctcctggt gcagtcggga cgacgaaccc tgaccaagag
     4801 ccgcatcacc gccgatcgcc agatcctgat ccgcgtcgat cacggcgaca cggggccgat
     4861 cgaccggttc accgagcggg ccctcctgga tcgcctgacc gaggcgttcg tccagagccg
     4921 cgcggtgatc gtctccgact acggctacgg gctgatgacg cccacggtcg tggcgcacct
     4981 ggccgccctg cagcgggacc gggccaggat cctggtcctg gacgcgaagg atcccaccgc
     5041 gtacgtggac gtgggggtca ccgccgtgaa gccgaacttc gctcaggcca tgcggctgct
     5101 cggggtcccc gacggggaca ccggccaggg cttcgatcgc gcggccagcg tgatcgaggt
     5161 gggcgaccgg ctgctggagc tcctgggcgc cgagatcgtg gcggtgaccc tcgacgcgga
     5221 gggcgcggtg atcctggagc gcggacgccc ggcccatcgc acctacgccg atccggtccg
     5281 gtcctcgacc acggcggggg ccggggacac gttctcggcg gcgttcgcgc tggcgctggc
     5341 cgtgggagca cagaccccca ccgcggccga gatcgcctcg gcggccgcct cggtggtggt
     5401 ggccaaaccc ggcaccgcgt cgtgcacccc caccgagctc cggcaggtcc tctcgccggc
     5461 cgccaagcgc ttggaccggg agggcctcgg cgagcggatg gaggcctatc gccggcaggg
     5521 caagcggatc gtgttcacca acggctgctt cgacatcctg caccggggtc acatcgccta
     5581 cctgaaccgc gccaaggagc tgggagacgt gctggtgatc ggggtcaacg acgatgccgg
     5641 ggtccgccgg ttgaagggcc cggaccgacc gatcaacgcg ctggaggacc ggatcgaggt
     5701 gctggccgcg ttgagctcga ccgatcacgt cgtgccgttc cgggaggaga cgccggccga
     5761 actgatccgg gcgatccgac ccgacgtctt cgtcaagggc ggggactact cggtggagcg
     5821 gctgccggag gcctcgttgg tgcaggccct cgggggccgg gtctggatcc tgccgttcgt
     5881 ggaggaccgg tccaccaccg ggatcatcga gcgcatccgc cgctccggcg gcgggtcgct
     5941 cgaccgggcg ccggcctgag atgcgcgggg cggggaactg ggcgggggcg cggaacctgc
     6001 tgtgcatccg gctggacgcc ttgggcgacg tcctgatgac cgagccggcg atccgcgccc
     6061 tccgaggctc gctcgcgagg agccgcatca cgctgctcac gtccacgtcg ggagccgagg
     6121 cctgcggcat gatcgccgcc atcgacgacg tgatcgcgta cgaccctccc tggatgaggt
     6181 ccggctccgg gcaacggcgc cgcgaggacc gggggatgat cgaccgcctc gctcgccacc
     6241 ggttcgacgg cgcggtcatc ttcaccgtgt acagccagag cgccttgccc gccgcgtacc
     6301 tcacccacct ggccgggatc ccccggagcc tcgcgtacgg ccgggagaac ccctatcacc
     6361 tgctgaccga tcaccgggcc gaggatgaac cccaccggat cgtccgccac gaggtccgcc
     6421 gccacctcga cctggtggcc tcgatcggct gctccacgcc cgacgagcgg atccacgttc
     6481 gtccctctcc ccgggcccgc cggtcgatcc gcgaacggat cggcgcgatg ggcctccgac
     6541 gagacgagcc gtgggtggtg gtccatgcgg gcgccacggc cccctcgcga cggtacccgg
     6601 tcgagcgctt cgcggaggtc gtcgcgcggc tgcgccgcga tctgcgatgg ccggtggtgc
     6661 tcaccggctc ggagaccgag c
//
LOCUS       DASQQQ010000129         4457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_258845, whole genome shotgun sequence.
ACCESSION   DASQQQ010000129 DASQQQ010000000
VERSION     DASQQQ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258845"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="VE646_05450"
     CDS             complement(<1..1237)
                     /locus_tag="VE646_05450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZD79466.1"
                     /translation="MGHRGTIGDPEEGSLIGPDRPQRLEAEGSAVPRNRYVDLLRVGA
                     IALVALGHWLLTSLSYRQGHFVIGDVLGRIRWARWLTLGFQVMPVFFLVGGFANAISW
                     SAHRDEGWTAWLHRRVRRLLWPTTLFVATALVVVTGCRLVGVDPTDLSQGAWAVAIQL
                     WFLPVYLLLLLGTPAMYAAHRRWGLWMPATLILGAGLVDALVLGLGWSWMGWANYLLV
                     WGAAHQLGFAWHDGGLTHRRWMPLALAGVGAGALAALVGLGPFPLSMIGVPGAPIDNA
                     APPTVALAALAVCQTGVVVAMAGRAARLLTRPPLWRFVQRANASVMTIYLWHMVPVIP
                     LAVALYPSRVVVEPSIGSASWWWMRLPWLAMLAALTVPLVIAVGRLEYRGLALTAPPR
                     SERRSTWMLLTGVGAVTFAL"
     gene            complement(1558..1839)
                     /locus_tag="VE646_05455"
     CDS             complement(1558..1839)
                     /locus_tag="VE646_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79467.1"
                     /translation="MEVLAQYAQQSGGGDAALALVCLGFWALIVWGTYRMAVRNGRSG
                     GLAIVLGLIFGIWALLGYAIAGPTSEMRARGRQSVPVPVPPPPPPNTLP"
     gene            complement(2161..2234)
                     /locus_tag="VE646_05460"
     tRNA            complement(2161..2234)
                     /locus_tag="VE646_05460"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2198..2200),aa:Ile,seq:cat)
     gene            complement(2305..3120)
                     /locus_tag="VE646_05465"
     CDS             complement(2305..3120)
                     /locus_tag="VE646_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HZD79468.1"
                     /translation="MLSVVTLLIIVTLSLLVTRVATVALTLTGMSRETARFQARSALT
                     GTGFTTREAESVVSHPVRRRIIRLLMLLGSAGLVTAMASLLLSFTSVGSGLAGLKRAV
                     LLLAGLGLLWIFTLSDRVDRWMSVVIERVLRRSTDLQVRDYEGLLRLAGGWVVAELKV
                     SDEDWLADRTLADLDLPQEGVLVLGIERVDGRYVGAPKGSAALHPGDVVILYGHERVV
                     VDLDVRESGLRGERDRRGSRLLFRRHLAAQEREEEEHREAGSEARSSVGRSSP"
     gene            complement(3974..4046)
                     /locus_tag="VE646_05470"
     tRNA            complement(3974..4046)
                     /locus_tag="VE646_05470"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4011..4013),aa:Asn,seq:gtt)
     gene            4132..4365
                     /locus_tag="VE646_05475"
     CDS             4132..4365
                     /locus_tag="VE646_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79469.1"
                     /translation="MLRRLLWIGVGIAIGYQLATRTSHVTEAASSNPAVRELADRGRS
                     LADRAGVVGLRAVQRARVEIQHRLAASGDADRD"
BASE COUNT          731 a         1623 c         1484 g          619 t
ORIGIN      
        1 acagcgcgaa cgtcacggcc cccacccctg tcagcagcat ccaggtggac cggcgctcgg
       61 accgcggcgg agcggtgagc gccaggcctc ggtactcgag tcggcccacc gcgatcacca
      121 gcggaaccgt cagcgccgcg agcatcgcga gccaggggag ccgcatccac caccacgagg
      181 cggatccgat cgaaggctcc accaccaccc gcgatgggta cagggccacc gccagcggga
      241 tcaccggcac catgtgccac aggtagatcg tcatcaccga cgcgttcgcc cgttgcacga
      301 agcgccacag cgggggacgg gtgagcagac gcgcggctcg gcccgccatc gccaccacca
      361 cccccgtctg gcagacggca agagccgcca gcgccacggt cgggggcgcc gcgttgtcga
      421 tgggggctcc gggcaccccg atcatgctga gcgggaacgg acccaggccc accagggcgg
      481 ccagggcgcc ggcacctacc cccgcgagag ccagcggcat ccatcgccga tgggtgaggc
      541 cgccgtcgtg ccaggcgaag ccgagctggt gggcggctcc ccacaccagc agatagttgg
      601 cccatcccat ccagctccag ccgagcccca ggaccagggc gtccacgagc ccggcaccga
      661 ggatcagcgt ggccggcatc cacagacccc accggcggtg cgccgcgtac atcgcggggg
      721 tgcccaacag caagagcagg tagacgggga ggaaccacag ctggatcgcg acggcccacg
      781 cgccttggga gaggtcggtt ggatccaccc cgaccagacg gcaccccgtg acgaccacca
      841 gcgccgtggc gacgaagagc gtcgtcggcc acaggagcct cctgacccgc cggtggagcc
      901 aagcggtcca gccctcgtcg cggtgcgcgg accaggagat cgcgttggcg aacccgccca
      961 ccaggaagaa caccggcatc acctggaacc ccagggtcag ccagcgcgcc caccggatcc
     1021 gtcccaggac atcgccgatc acgaagtggc cctgtcggta cgagagcgag gtgaggagcc
     1081 agtgtccgag cgccaccagg gcgatggcgc cgacccggag cagatccacg tagcggttgc
     1141 ggggcaccgc ggacccttcg gcctccaagc gttgcggacg atccgggccg atgagcgacc
     1201 cctcctccgg atcgccgatc gttccccgat gacccatgcg cgatcccttc gccttcaagg
     1261 ataggagtac cgcgcccttg cgacgaccgc ctcccgcacc gagtcaccca gatccgagcc
     1321 cgatccacga gggctcgggc gccccgcctc agcagccgag cgggaacgcc actgccaccg
     1381 acgtgggaga ggagcaagca gccgctccgg accaggggcg cggcctcccc gcgtggggat
     1441 cccgtcggct cgtgatcggc gctcgcggct agcgcgggtc accaggccga tcgcaggtac
     1501 ttgctcaggc tggatcccag ccgaccgtac ggcctacccc tgccgcttgc gggttcttca
     1561 cgggagcgtg ttcggtggtg gcggcggcgg caccggcacc ggcaccgact gccgcccgcg
     1621 ggccctcatc tcggacgtcg ggcccgcgat cgcgtagccc agcagggccc agatgccgaa
     1681 gatgagaccc aagacgatcg cgaggccacc gctccggccg ttgcgcaccg ccatcctgta
     1741 ggtcccccag acgatgaggg cccagaagcc gagacacacc agcgccagcg cggcgtcccc
     1801 gccgcccgac tgctgcgcgt actgcgcgag cacctccaca cctcctcctc gcgatccagt
     1861 tgctcgttat cgcttcggct gaagagagcc gtcacttgat ggagggcgac cccgagagcg
     1921 tgggattgcc ttgggccagg caggtcacga gcatcggaac cgccgtaggg tcgccacgct
     1981 ccagccgcct ctggccgtgc ccccgctcct gggctcgaag gcggctgctc gagagggacg
     2041 tcggccaccc atccgtgaac ccgcgtgggt ggcggcgagg acgtcgctcg ttcgaagcct
     2101 cgggtctcct gcagcaccgc cagccggcct cgtctgatcc gccggcgggt ctttccctcg
     2161 tggggcgggc aggactcgaa cctgcgaccg gcggattatg agtccgctgc tctgaccgac
     2221 tgagctaccg ccccagggcc tgctctcgac cccttcgagc gtacatggtg ggtacgggga
     2281 agcaccctgt gaaaccgtct gttctcaagg tgagctcctt ccgacggagg agcgagcctc
     2341 cgaaccggct tctcggtgct cctcttcctc ccgctcctgg gccgcgaggt gacggcggaa
     2401 cagcagccgc gagccccgtc gatcgcgctc gccacgcagc ccggactctc gcacgtccag
     2461 atccaccacc acgcgctcgt gcccgtagag gatcaccacg tcgccgggat gcagcgcagc
     2521 cgatcccttc ggcgcgccca cgtatcgccc atcgactcgc tcgatcccca gcaccagcac
     2581 accctcctgt ggaagatcca ggtcggccag cgttcggtcg gccagccagt cctcatccga
     2641 gaccttgagc tcggccacca cccacccgcc ggccagacgc agcagccctt cgtaatcacg
     2701 gacctgcagg tccgtggacc ggcgcaacac gcgctcgatc accacggaca tccaccgatc
     2761 cacccggtcg ctgagggtga agatccacag caagcccagt ccggcgagga ggagcaccgc
     2821 gcgcttcagg cccgccaggc cgctgcccac ggacgtgaac gacagcaaca gggaagccat
     2881 cgcggtgacc agcccggcgc tgcccaggag catcagcaac cggatgatgc ggcgccggac
     2941 cggatggctg accaccgact cggcctccct ggtggtgaat ccggtaccgg tgagggcaga
     3001 gcgtgcctgg aagcgtgccg tttccctgga catgccggtc agcgtgaggg cgaccgtcgc
     3061 cacgcgggtg accaacagcg acaacgtaac gatgatcagc aacgtcacga ccgagagcat
     3121 cccccacctc ctgtacgggc agtacccgac ggtgacgtcg ccgaaccgag gccggaaggg
     3181 tgggccgctc gaggaggccg cgaaccgcgc cactccttcg tgcggaaggt aaccttcacg
     3241 ggtggtggaa cccgaggcag gaaggaacag tccctaccgc cgtgtgctgg tgacggcctg
     3301 gggagccgcg gccgtatgct tggcggtgtt cctggctatg ctcgtgatgg ccctgcacgc
     3361 cggtggcacg cgaagcctgg agcagtggat cagcgaccgc atccccttcc ggcaggcgcc
     3421 gaaggcggtg gagttcatcg agcgtctggc ggccatcccg ggctcgagac ggggcgccct
     3481 gcttctcgtg ctcgtcgtct tcgtctggtg gtgggtgcga caccgggacc tccgacccgg
     3541 cgtgctcctg gcggcggcgt tcacgggcac cacctcgacc gttgccatcc tcaagccgtt
     3601 gatcgtccgc gcctccccgc tccaggggag cggggtgggc cacgccttcc cctccctcca
     3661 cacggccgat gcggcggccg tcctcggcac gctggcgatc gtggtcgcgc tatctcaccg
     3721 acggctcttg gttcgctcca tggtgatctc cgctgctctg gtgaccgtgg tcgggctggc
     3781 ggtgatggcg cagggttatc actggttcac ggacgtggtg gccggcgcgg ctgtggccgg
     3841 tgtgtgggtg ttcggcctca ccccggccgc gtacctgatg tggacgcgtc cggacctggt
     3901 gcacgcgctg catcgggatc ggcttccgga ggtcgccgga tccctggggc ggctacggct
     3961 catgggatca cagctccggg ggggagactc gaactcccaa ccatcggatt aacagtccgc
     4021 cgctctgccg attgagctac cccggaagat gccggagcag tctaccaacg gcgcatcggc
     4081 gcccggcccg agctatgctc ccgtcgaccc cgaccggaaa ggatcggaac catgctgcga
     4141 cggctccttt ggatcggtgt gggtatcgcc atcggctacc agctcgccac ccgaacctcg
     4201 cacgtgaccg aggccgcctc gagcaatccg gccgtccggg agctggccga tcgcggccgt
     4261 tcgctcgccg atcgggccgg cgtggtgggg ctgagggccg tccaacgggc ccgcgtggag
     4321 atccagcacc ggctcgccgc cagcggcgac gccgaccggg actaacaact agtctcctga
     4381 gtcggaagtt cacatgatgt tcacgaggat gtcctcagcc gtggttcgcg gcgtgcaagg
     4441 atgccgcatg gccagag
//
LOCUS       DASQQQ010000130         7178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_341348, whole genome shotgun sequence.
ACCESSION   DASQQQ010000130 DASQQQ010000000
VERSION     DASQQQ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341348"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VE646_05480"
     CDS             complement(<1..691)
                     /locus_tag="VE646_05480"
                     /inference="COORDINATES: protein motif:HMM:NF020919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1996 domain-containing protein"
                     /protein_id="HZD79470.1"
                     /translation="MPLRRNAVTAGRPGGPRERRTGGSRTPRPTAHPGLLLAVITALV
                     GGALLPPALAATGGGQFVIRCRYSHSLRDDPIVFPGQPGASHRHDFYGNRSVDASSTQ
                     ATMLSATTTCRVPSDTAGYWVPATYLAGERVIPPVARLYYLGTAGRTVETIPPGLEMI
                     GGDKTAASPQENLHVRWYCGEIASVKTPRRDTPYDCTPYAQYSFVDGVVAIIDMPNCW
                     DGTGLEPADVVY"
     gene            750..1568
                     /locus_tag="VE646_05485"
     CDS             750..1568
                     /locus_tag="VE646_05485"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HZD79471.1"
                     /translation="MIPYKTFPVFHVGPVAFHTFGVFVGLGMLVGAWVFVRHGRRAGM
                     DAEELGALAWWILIAGFVGARLMYVIAHIGDFVSRPWAVVAVWEGGLEFSGGFIAAVA
                     VVLWWLRRHRDVPGLTLADGVVLGLAPGLAIGRIGCYSVGEHLGGSTSFPLAVHYLGG
                     VTREGPIAIGAHIHNTALYEILLLLPLIALLFWLRHRGVGPGWLTVTFLLWYGVQRFC
                     TDFLRAYDRTVLGLTGAQYLCIVLVGAGLVLMTRIRRSSQEADRLPSAAPAEAH"
     gene            complement(1604..2728)
                     /locus_tag="VE646_05490"
     CDS             complement(1604..2728)
                     /locus_tag="VE646_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014058165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZD79472.1"
                     /translation="MEGTPTLHQTLVALGSVLVGLAAGWAGRWLFGRLEQRAQRTPWA
                     GDEALYAALGETSLAGFAVTGVWAAALALPLRDHVRSIVDASLLSLIILLITVVCARL
                     TAGLIRAYTRQRPGVAGSSSIFVNITRLLVFAIGLLVLLQTLGVSIAPLLTALGVGGL
                     AVALALQDTLSNLFAGLHILASKKVQPGDYIQLDSGQEGYVVDINWRNTWIRALPNNM
                     VLVPNARLAQAIVTNFYQPEREMSVLIEVGVAYGSDLEQVERVTIEVAREVMRGVSGG
                     VPSSDPFIRYHTFNESSIDFSVILRAKEYTDQYVIKHEFVKQLHDRYLDEGITIPFPI
                     RTIGFVEPGRDGADPHRNVAASSGRSGTPEEGADGGRSGS"
     gene            2810..3562
                     /locus_tag="VE646_05495"
     CDS             2810..3562
                     /locus_tag="VE646_05495"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HZD79473.1"
                     /translation="MRTTLGARPVALLTGPTLVLMLVLGLPATLQAPGARAVAAVRPS
                     TLRPPLVWKKIPFGAKRKRQMAAYSKRHYGLRTWRLADPHVIVEHYTDGTSVRSAWSY
                     FAANVRHLGEMPGVCAHFLVDTDGTVYQLVNLGIRCRHAIGMNWTAIGIEMVGTSDAQ
                     ILHRRVQLRAALRLTLWLMARFGIGVGNVIGHAETLQSPYHHERYAAWRCLTHADWQH
                     PDMRTFRHRLRRMAQRAGVPAGPGPVWVDSGC"
     gene            3737..5146
                     /locus_tag="VE646_05500"
     CDS             3737..5146
                     /locus_tag="VE646_05500"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HZD79474.1"
                     /translation="MLAAGGALWLVRTGRAEHRSLPGSPPASPAHVSPSVSPSPAYHL
                     RVVLGSLKGHRVQGVPVRQRDLRAPARAIRGTLTQLYSIGFVDPSRWDGGRFPALYRL
                     FSRDARSGVHRDLSDLSVGRLATRLSAVRPRRARLDLRFLLDRGGHPVTAVARMRFSG
                     TALADGIQAAIRQDGRYVLRRMAGRWRIVSYRVRSHQPSLPDLRGTPGRAAYSPALPS
                     SGLLFVLVIGSDARPGQSVAATRADSLHIIGVNPKLGMASVLGIPRDSYVPIPGAGVQ
                     KINSALVHGGPELMVRTVEQLSGIHIDAYVLTGFDGFRSLVNAVGGIRTTVPYPMNDH
                     YSRAHFRKGPTRMNGREALAFARDRHDVPGGDVGRSRNQGRLIVSAFREFRTDVQRNP
                     LELLRWVAAGSRYLRTNLTLQQMTELLLAVPSIDAKHVRNDVVPGTGGSAGGQSVIRL
                     GSAAHSLFRDLGSDGVLGR"
     gene            5235..6191
                     /locus_tag="VE646_05505"
     CDS             5235..6191
                     /locus_tag="VE646_05505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZD79475.1"
                     /translation="MAQPSQDDRIPQTADRPDAPGSPRDLPRTSWTTALKATIAAMRK
                     DRVPLVAAGMGFYWFLSVFPLLIAAIGIIALAHVGSSMVTTINDAIHTALPGEAAGVL
                     TTAVSRAQTQTGGGLAALVVSVLVAVWSASSGMAAMQEGLDVAYGQESRKFLKKRLVG
                     IVLILATLVLGGLAMALIVFGTPLGVWVLDHVPDGHSLVWAWTVVRWVLAIPVIMALF
                     GLFYRLGPNRPQPAASWLSPGAIVGAGIWVAVSLGFSFYISNFGGSYGDTYGALAGVV
                     LLLLWLYLTAIAVLLGGELNGELERQRARRMGIRAAPDAPAA"
     gene            6306..>7178
                     /locus_tag="VE646_05510"
     CDS             6306..>7178
                     /locus_tag="VE646_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014182309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZD79476.1"
                     /translation="MRDFDLYIDGRWATGEGPDRIQVRDPATAEPVWTVVRGTAGDID
                     RAVRLARAAFEDGRWSRIAPGERARMLLRVADLIDARMPELARIESVDAGKPIKLTTN
                     FDIPFGADNVRFFAGAARNLEGKAAGEYSPDHLSYIRREPLGVIAAICPWNYPFQIAV
                     WKIFPALAAGNTVVVKPAKETPATAFEIAVLCQEAGIPAGVLNVVTGYGAEIGEELVR
                     HPLVDMACVTGDTETGRKVMRAAAEAPKKVHLELGGKAPFIVFDDADLEAAARGAVAG
                     AYINCGQDCTAATRA"
BASE COUNT          978 a         2485 c         2578 g         1137 t
ORIGIN      
        1 cgtagaccac gtccgccggc tccagcccgg tgccgtccca gcagttgggc atgtcgatga
       61 tcgccaccac cccgtccacg aacgagtact gcgcgtaggg tgtgcagtcg tacggcgtgt
      121 cgcgccgagg ggttttcacg ctggcgatct cgccgcagta ccagcggacg tgaaggttct
      181 cctgcgggga ggcggccgtc ttgtccccgc cgatcatctc cagccccgga gggatggtct
      241 ccacggtccg gccggcggtt cccaggtagt acagccgggc caccgggggg atgacccgtt
      301 ctccggccag gtacgtggcc ggcacccagt agccggccgt atcgctgggt acccggcagg
      361 tggtggtggc cgacaacatc gtcgcttggg tggacgaggc gtccacggaa cggttgccat
      421 agaagtcgtg ccggtgcgat gcgcccggtt gcccggggaa cacgatgggg tcgtcccgga
      481 gcgagtgcga gtagcggcac cggatcacga actgcccgcc tcccgtggcc gcgagggcag
      541 gcggcaggag cgcgccgccc accagggctg tgatcaccgc gagcagcaga ccggggtggg
      601 ccgtgggtct cggggtccgg gatccgccgg tccggcgctc ccgggggccg cccggtcgac
      661 ctgcggtcac ggcgttgcgt cgaagcggca ctcatggcat cctaccggcc tcggtcggac
      721 gggggaagcg ccggtagcat ggaggggccg tgatcccgta caagaccttc cccgtgttcc
      781 acgtggggcc cgtcgcgttc cacacgttcg gggtcttcgt cgggctgggc atgctggtag
      841 gcgcctgggt gttcgtccgc cacggccgcc gggcggggat ggacgccgag gagctgggcg
      901 cgctggcctg gtggatcctg atcgccgggt tcgtcggggc tcgcctgatg tacgtgatcg
      961 cgcacatcgg cgacttcgtg tcccggccct gggccgtggt ggccgtctgg gagggcgggc
     1021 tggagttctc cggcggcttc atcgcggcgg tggcggtggt gctgtggtgg ctgcggcggc
     1081 accgggacgt cccggggctc acgctggccg acggcgtggt gctcgggctg gcgccagggc
     1141 tggcgatcgg ccggatcggc tgctactcgg tgggtgagca cctgggtggg tcgacctcgt
     1201 tcccgctggc cgtgcactac ctgggcgggg tgaccaggga gggacccatc gcgatcgggg
     1261 cgcacatcca caacaccgcg ctgtacgaga tcctgctgct cctgccgctg atcgccctgt
     1321 tgttctggct ccggcaccgt ggggtcgggc ccggctggct caccgtgacc ttcctgcttt
     1381 ggtacggcgt gcagcgcttc tgcaccgact tcctccgggc ctacgaccgc accgtcctcg
     1441 gcctgaccgg ggcgcagtac ctgtgcatcg tcttggtggg ggccgggctg gtcttgatga
     1501 cccggatccg ccgctcctcg caggaggctg atcggctgcc ctcggctgca ccggccgagg
     1561 cgcactgacc catccgggtc cgggcccggt ggatcggaac gggtcaggag ccggagcggc
     1621 ccccgtccgc gccctcctcc ggggtcccgg aacgcccact gctcgcggcc acgttccggt
     1681 ggggatcggc gccgtcccgc cccggctcca cgaacccgat ggtgcggatg gggaacggga
     1741 tggtgatgcc ctcgtccagg taccgatcgt ggagctgctt cacgaactcg tgtttgatca
     1801 cgtactggtc cgtgtactcc ttggcccgca ggatgacgga gaagtcgatg ctggattcgt
     1861 tgaaggtgtg gtagcggatg aaggggtcgc tggagggcac gccgccgctc accccccgca
     1921 tcacctcccg ggccacctcg atcgtcaccc gctcgacctg ctccagatcg ctcccgtacg
     1981 ccaccccgac ctcgatcagc accgacatct cccgctcggg ctggtagaag ttcgtcacga
     2041 tcgcctgcgc cagccgggcg ttcggcacca ggaccatgtt gttgggcaag gcgcggatcc
     2101 acgtgttccg ccagttgatg tccacgacgt aaccctcctg acccgagtcc agctggatgt
     2161 agtccccggg ctggaccttc ttcgatgcca ggatgtgcag gccggcgaac aggttggaga
     2221 gggtgtcctg cagcgccagc gcgacggcca ggcctcccac acccagcgcg gtgagcagcg
     2281 gcgcgatcga gacccccagc gtctggagca gcaccaggag gccgatcgcg aacaccagga
     2341 ggcgcgtgat gttgacgaag atcgagctgg agccggccac ccccgggcgc tggcgcgtgt
     2401 aggcgcggat cagcccagcg gtcagccgag cgcagaccac ggtgatcagc aggatgatga
     2461 gcgacagcaa cgaggcgtcc acgatcgagc gcacgtgatc gcgcaagggc agggccaacg
     2521 ccgccgccca gacgccggtc acggcgaacc ccgccagcga ggtctctccg agcgcagcgt
     2581 agagggcttc gtccccggcc cacggggttc gctgagctcg ctgctccagg cggccgaaca
     2641 gccaacggcc ggcccagccc gccgcgagtc cgaccagcac cgatccaagg gccaccagcg
     2701 tctggtggag cgtgggagtt ccctccacgc gcatcacctc cggtccacga ccctaccggg
     2761 tccggggggc ccgtttcaca tccaccccga cgtgagacag gatgggcccg tgagaacgac
     2821 ccttggtgcc aggccggtcg cgttgctgac ggggccgacg ctcgtgctga tgctcgtgct
     2881 ggggctgccg gcgaccctgc aggccccggg cgcgcgcgcc gtcgcagcgg tgcgcccctc
     2941 gacccttcgg ccgccgctgg tctggaagaa gatccccttc ggggccaagc gcaagcgaca
     3001 gatggccgcc tattcgaagc gtcactacgg ccttcggacc tggcggctcg ccgacccgca
     3061 cgtgatcgtc gagcactaca cggacgggac ctcggtccgg agcgcctgga gctacttcgc
     3121 cgccaacgtc cggcatctcg gcgagatgcc cggggtgtgt gcgcacttcc tggtggacac
     3181 cgacgggacg gtctaccagc tggtgaacct ggggatccgg tgccgccatg ccatcgggat
     3241 gaactggacg gcgatcggga tcgagatggt ggggaccagc gacgcgcaga tcctccaccg
     3301 gcgggtgcag ctgcgggccg ctctgcgcct caccctctgg ctgatggccc gcttcggcat
     3361 cggcgtgggc aacgtgatcg gccacgccga gaccctccag agcccctacc accacgagcg
     3421 gtacgcggcc tggcgctgcc tcacgcacgc ggactggcag cacccggaca tgcgcacgtt
     3481 ccggcaccgc ttgcggcgca tggctcagcg cgccggggtc cctgcggggc cgggtcccgt
     3541 gtgggtcgac tccggctgct gagctcggcg gggccccgga tgctgccgcg acctcgccca
     3601 tggatcggct tgcacgcgat ggctcggccg gtagtctgcg ggccatgcac gtcttgcccg
     3661 tcccgctccg agtgggcacc gtctccccgt caagctccgt cctcgaacgc tggtcctggg
     3721 atcgttggtg gcggtcatgc tggccgcggg tggagcgctc tggctggttc gtaccgggcg
     3781 cgcggagcac cggagcctgc cgggctcgcc accggcttcc ccggcgcacg tctctccgag
     3841 cgtgagcccg agtcccgcct accacctccg cgtggtgctg gggagcctga agggccatcg
     3901 ggtgcaggga gtacccgttc gacagcggga cctgcgggca ccggcgcgag cgatccgcgg
     3961 caccttgacc cagctgtact cgatcgggtt cgtcgatccc agccggtggg atggggggcg
     4021 gttcccggcc ctgtaccggc tgttctcacg cgatgctcga tcgggcgtcc accgtgacct
     4081 ctccgacctg tcggtgggcc ggctggcaac gcgcctgtcg gcggtccgtc ctcgacgggc
     4141 caggttggat cttcggttcc tgctggatcg gggcggtcac ccggtgaccg cggtggcgcg
     4201 catgcggttc tcgggcaccg cgctggcgga cgggatccag gccgccatcc ggcaggacgg
     4261 ccggtacgtg ctccgccgga tggccggccg ctggcggatc gtctcctacc gggtccgcag
     4321 ccatcagccc tcgctcccgg acctgagggg cacgccggga cgtgccgcgt actcgccggc
     4381 cctgccgtcc tccggtctgc tgttcgtcct ggtcatcggc agtgacgccc gtcccgggca
     4441 gtcggttgcg gcgacgaggg cagattccct gcacatcatc ggcgtgaacc cgaaactcgg
     4501 gatggcttcg gtgctgggga tcccgcgcga ctcctacgtc cccatccccg gggcgggggt
     4561 ccagaagatc aactcggccc tggtgcatgg agggccggag ctgatggtcc gcacggtcga
     4621 gcagctcagc gggatccaca tcgacgcata cgtgctcacc gggttcgacg gcttccgcag
     4681 cctggtgaac gcggtcggcg ggatcaggac cacggttccg tatcccatga acgaccacta
     4741 ctcgcgcgcc catttccgaa agggccccac ccggatgaac gggcgggagg cgctggcctt
     4801 cgcccgtgac cggcacgacg tgcccggggg agacgtgggc cgctcccgca accaggggcg
     4861 tctgatcgtc tcggccttcc gggagtttcg tacggacgtc cagcgcaacc cgctggagct
     4921 cctgcggtgg gtggcggccg gttcgcggta cctgcgcacg aacctcaccc tccagcagat
     4981 gacggagctc ctcctggcgg tgccatcgat cgacgccaag cacgtacgga acgacgtggt
     5041 gccgggaacg ggtggatccg ccggcggtca gagcgtgatc cggctcggct cggccgccca
     5101 ctccctgttc cgagacctgg gctctgacgg cgtgctcggc cgctgatccc gggccgacac
     5161 gaatgggcca cgcccatgag ggtatccacc gagggatatc gggctagccg tccaaacgcc
     5221 gagaggaggg tggcatggct cagccatcac aggacgatcg catcccccag accgccgacc
     5281 gaccggacgc cccagggtcc cccagggacc tgccgaggac cagctggacc acggcgttga
     5341 aggcgacgat cgccgccatg cggaaggacc gggtacccct cgtggccgcc ggcatgggct
     5401 tctactggtt cctatccgtc ttcccgctcc tgatcgccgc catcgggatc atcgcgctgg
     5461 cgcacgtggg ctcctccatg gtcaccacga tcaacgacgc catccacacc gcgctcccgg
     5521 gcgaagcggc cggcgtgctc accaccgccg tgtcccgagc gcagacccag accggcggcg
     5581 gtctggcggc gctcgtggtc agcgtcctcg tcgcggtgtg gagcgcctcg tcggggatgg
     5641 ccgccatgca ggagggcctg gatgtggcct acgggcagga gagccggaag ttcctgaaga
     5701 agcgcctggt cgggatcgtg ttgatcctgg ccacgctcgt gctgggcggt ctggccatgg
     5761 cgctgatcgt gttcggcacc ccgctggggg tgtgggtcct ggaccacgtt cccgacggcc
     5821 actcgctggt gtgggcatgg accgtcgtgc gttgggtgct ggccatcccc gtgatcatgg
     5881 cgctcttcgg cctcttctac cggctcggtc cgaaccggcc acagccggcg gcgtcgtggc
     5941 tgagtcccgg tgccatcgtg ggcgcgggga tctgggtggc cgtgtcgctg gggttctcct
     6001 tctacatctc gaacttcgga ggctcctacg gcgacacgta cggcgccctg gccggcgtgg
     6061 ttctcctctt gctctggctg tacctgaccg cgatcgccgt cctgctcggt ggagagttga
     6121 acggtgagct cgagcggcag cgggcccggc ggatgggcat ccgcgccgcc cccgacgcgc
     6181 ccgcggcctg aagaccatcc cctggacgca cgcgggtccc ggccgtcgaa ggtcctccgc
     6241 ccctggagcg ccgggccggc tccggcaggc tgtgagcacg ggcatcccgt ggggggaggt
     6301 tgaccatgcg cgacttcgac ctgtacatcg acggccggtg ggcgacgggc gagggacccg
     6361 atcggatcca ggtccgcgac ccggctaccg cggaaccggt gtggaccgtc gtccggggca
     6421 cggccgggga catcgatcgc gccgtgcgct tggctcgcgc ggcgttcgag gatgggcgct
     6481 ggagccgcat cgcccccggc gagcgggccc ggatgctgct cagggtcgcg gacctgatcg
     6541 acgcccggat gccggagctg gcgcggatcg agtccgtcga cgccggcaag ccgatcaagc
     6601 tgaccacgaa cttcgacatc cccttcgggg ccgacaacgt ccggttcttc gcgggcgcgg
     6661 ctcggaacct ggagggcaag gcggccggtg agtactcgcc cgaccacctg agctacatcc
     6721 ggcgggagcc cctgggcgtg atcgcagcca tctgcccctg gaactacccg ttccagatcg
     6781 cggtgtggaa gatcttcccg gccctggcgg ccgggaacac ggtggtggtg aagccggcga
     6841 aggagacccc ggccaccgcc ttcgagatcg ccgtgttgtg ccaagaggcc gggatccccg
     6901 ccggcgtgct gaacgtggtg accggatacg gtgcggagat cggtgaggag ctggtccggc
     6961 accctctcgt ggacatggcc tgcgtcacgg gggacacgga gaccgggcgc aaggtgatgc
     7021 gtgcggcggc cgaggcaccg aagaaggtcc accttgagct ggggggcaag gccccgttca
     7081 tcgtcttcga cgacgccgac ctggaggcgg ccgccagggg cgccgtggcc ggcgcctaca
     7141 tcaactgcgg ccaggactgc acggcggcca cccgcgcc
//
LOCUS       DASQQQ010000131         3204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_175949, whole genome shotgun sequence.
ACCESSION   DASQQQ010000131 DASQQQ010000000
VERSION     DASQQQ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175949"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VE646_05515"
     CDS             complement(<1..473)
                     /locus_tag="VE646_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZD79477.1"
                     /translation="METTVDRERLRTLTRREEVDFVERHPRSGALFEQGKAHLLAGVP
                     MPWMTEWAGPYPVFVAEAQGARFVDVDGHEYVDLCLGDTGAMTGHGPKEAVAAIAEQA
                     AKGITLMLPSEDSIWVGAEMTRRFGLPYWQFCLTATDANRFTLRLARAITGRPKVL"
     gene            complement(603..2339)
                     /locus_tag="VE646_05520"
     CDS             complement(603..2339)
                     /locus_tag="VE646_05520"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HZD79478.1"
                     /translation="MTKSEERMGTLEHEWITALRTGKPAAVPIEGAALLEHPMLNKDA
                     AFTDNERDVFGLHGLLPVRVRSMEEQVQVEVEKIRRKSDPLEQYIGLAALQDRNETLF
                     YRVLIEHLEEFMPIVYTPVVGQACQTYSHILRRPRGLWLTPDDIDRIPELLRNARHED
                     VRLIVVTDNERILGLGDQGAGGMGIPVGKLSLYTAGAGIYPALTLPVSLDVGTDNEDL
                     LADPYYLGYRAPRLRGERYDAFIEAFVGGVLEVFPHTVLQWEDFKQHNAIRLLDRYRQ
                     RITSFNDDIQGTSAVVLAGILAALRYLGEPLGAQRFVFLGAGAAGIGIARLLRAAMQR
                     EGIPEDDIRRAIVQLDSKGLTFEGRTPLDEDKREFALRAEDLPALGFGPAERYSLETV
                     IRHVQPTMLIGTSGTPGSFTEAAIREMAKHVKSPVIFPLSNPTSKSEAVPEAVLAWTE
                     GRALVATGSPFDPVKLDGRTHLIGQANNAFVFPGIGLGAIVAQVRAITDDMFLAAADA
                     LAEMVPPERMEQGALYPSQSQLRAISRAIAKRIVCQARDCGIGRAFQEQELEATVDEM
                     IWYPDYVPYRPI"
     gene            complement(2336..>3204)
                     /locus_tag="VE646_05525"
     CDS             complement(2336..>3204)
                     /locus_tag="VE646_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HZD79479.1"
                     /translation="NPGHAVLFPIIVGVSLVFGVMLVVRIGGADMPTVISLLNGYAGL
                     AASMMGFVLSNKLLIIAGALDGSSGFFLSIIMCRAMNRSFTNVLFGGFGAEQVAAEIE
                     QRPVRSVDPEETAFLLDMARRVVIVPGYGMAAAQAQHKVRELSDALLHRGVDVRFAIH
                     PVAGRMPGHMNVLLAEANVPYDLLYDLESINPELPQTDVVLVVGANDVVNPAARSDEH
                     SPIYGMPILDVDRAQSVIVVKRSMRSGFAGVDNELFYLDKTQMLFGDAKQVIGSVVEE
                     LSKLPAREVAPA"
BASE COUNT          476 a         1145 c         1089 g          494 t
ORIGIN      
        1 aggaccttgg gccgtccggt gatggctcga gccagccgca gggtgaagcg gttcgcgtcg
       61 gtcgccgtga ggcagaactg ccagtagggg agcccgaacc gccgggtcat ctcggcgccc
      121 acccagatcg agtcctccga gggcagcatc agggtgatcc ccttggcggc ctgttcggcg
      181 atcgcggcca cggcctcctt aggtccgtgc ccggtcatcg cgcccgtgtc ccccaggcag
      241 aggtccacgt actcgtgccc gtccacgtcc acgaagcggg ccccctgagc ctcggccacg
      301 aagaccggat agggccccgc ccactccgtc atccacggca tgggcacgcc ggccagcagg
      361 tgggccttgc cctgctcaaa cagcgcgccg gagcgcggat gacgctcgac gaagtccacc
      421 tcctcgcggc gggtcagcgt gcgaaggcgt tcacggtcga cggtggtctc catggtcgag
      481 ccccttcggg tcgcgggcgt tgcgatggaa ctgtgccccg ccgtgcccgg ggcgtcaacc
      541 gaacgtggga ggggccgcgc cctggcgcgg cccctcccgg cccccctcgg gcccccgccg
      601 gttcagatcg gacggtacgg gacgtagtcc gggtaccaga tcatctcgtc gaccgtcgcc
      661 tccagctcct gttcctggaa ggcccggccg atcccgcagt cccgggcctg gcacacgatg
      721 cgcttggcga tggcgcggga gatggcccgc agctgggact ggcttgggta cagcgcgccc
      781 tgctccatcc gctccggagg caccatctcg gccagcgcgt cggcggccgc caggaacatg
      841 tcgtcggtga tggcgcgcac ctgggccacg atggcgccga gcccgatccc ggggaagacg
      901 aacgcgttgt tggcctggcc gatcaggtgg gtgcgaccgt cgagcttcac cgggtcgaac
      961 ggactgccgg tggccaccag ggcccgaccc tcggtccagg cgagcaccgc ctcgggcacc
     1021 gcctcggact tggaggtggg gttcgacagc gggaagatca ccgggctctt cacgtgcttg
     1081 gccatctccc ggatcgccgc ctcggtgaag ctgccgggcg tgccgctagt gccgatcagc
     1141 atggtgggtt ggacgtgccg gatcaccgtc tccaggctgt accgctcggc cggcccgaag
     1201 cccagcgcgg gcaggtcctc ggcccggagc gcgaactccc gcttgtcctc gtccagcggc
     1261 gtgcgaccct cgaacgtgag gcccttggag tccagctgga cgatggcccg gcggatgtcg
     1321 tcctccggga tgccctcgcg ctgcatggcc gcacgcagca ggcgggcgat cccgatcccg
     1381 gccgcgccgg cccccaggaa gacgaaccgc tgggccccga ggggctcacc gaggtaccgc
     1441 agcgccgcca ggatgcctgc cagcaccacg gccgaggtgc cctggatgtc gtcgttgaag
     1501 ctggtgatcc gctggcggta ccggtccagc agccggatcg cgttgtgctg cttgaagtcc
     1561 tcccactgca ggaccgtgtg ggggaagacc tccagcacgc ccccgacgaa ggcctcgatg
     1621 aacgcgtcgt accgctctcc ccggagccgg ggcgcccggt accccaggta gtaggggtcc
     1681 gccagcagat cctcgttgtc ggtgccgaca tcgagcgaca cgggcagtgt cagggccggg
     1741 tagatcccgg cgccggcggt atacagggag agcttcccca ccgggatgcc catgccgccg
     1801 gcgccctggt ccccgagccc caggatccgc tcgttgtcgg tcaccacgat cagccgcacg
     1861 tcctcgtgcc gggcgttgcg gagcaactcg gggatccgat cgatgtcgtc gggcgtgagc
     1921 cacaggccgc ggggccgccg caggatgtgg ctgtaggtct ggcacgcctg cccgaccacc
     1981 ggcgtgtaga cgatcggcat gaactcctcc aggtgctcga tcagcacccg gtagaacagc
     2041 gtctcgttcc ggtcctggag cgcggccagg ccgatgtact gctcgagcgg gtcgctcttg
     2101 cggcggatct tctccacctc cacctggacc tgctcctcca tgctgcggac ccggaccggg
     2161 agcagaccgt gcagaccgaa cacgtcccgc tcgttgtccg tgaacgccgc gtccttgttc
     2221 agcatggggt gctcgaggag cgccgccccc tcgatgggca ccgcggcggg cttccccgtc
     2281 cgcagcgccg tgatccactc gtgctcaagc gtccccatcc gttcctccga cttggtcatg
     2341 cgggcgccac ctccctcgcg ggtagcttgg agagctcctc cacgacgctg ccgatcacct
     2401 gcttggcgtc gccgaagagc atctgggtct tgtccaggta gaagagctcg ttgtccaccc
     2461 cggcgaagcc cgagcgcatc gatcgcttca ccacgatcac cgactgggcg cggtccacgt
     2521 ccaggatcgg catcccgtag atcgggctgt gctcatcgga tcgggccgca gggttcacca
     2581 catcgttggc ccctaccacc agcaccacgt cggtctgcgg gagctcggga ttgatgctct
     2641 cgaggtcgta gaggaggtcg tagggcacat tggcctcggc cagcaggacg ttcatgtgcc
     2701 cgggcatccg cccggccacc gggtggatcg cgaaccgcac gtccaccccg cggtgcagca
     2761 gcgcgtcgga gagctcgcgc accttgtgct gggcctgagc cgcggccatc ccgtacccgg
     2821 ggacgatcac cacccggcgg gccatgtcca ggaggaaggc cgtctcctcc ggatccacgc
     2881 tgcggaccgg tcgctgctcg atctccgcgg ccacctgctc ggcgccgaac ccgccgaaca
     2941 gcacgttggt gaaggaccgg ttcatggccc ggcacatgat gatcgacagg aagaacccgc
     3001 tggagccgtc cagggcgccg gcgatgatca gcagcttgtt gctgaggacg aagcccatca
     3061 tcgaggccgc cagccccgcg tagccgttca gcagcgagat cacggtgggc atgtcggctc
     3121 cgccgatccg gaccacgagc atgacgccga acaccagcga cacgccgacg atgatcggga
     3181 acagcacggc gtgaccgggg ttgg
//
LOCUS       DASQQQ010000132        11518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_204771, whole genome shotgun sequence.
ACCESSION   DASQQQ010000132 DASQQQ010000000
VERSION     DASQQQ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11518
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204771"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VE646_05530"
     CDS             complement(<1..561)
                     /locus_tag="VE646_05530"
                     /inference="COORDINATES: protein motif:HMM:NF015923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HZD79480.1"
                     /translation="MLGGSMGVRPGRVRRLRIRKAIGNAMLAVTMMTAGFLYLALVKN
                     VTLVVDGHPRSVRTLSANVGELLDARGIALAGTVLVEPPPATPLADGMTVVVDTDVSA
                     RPFLGPSSRDVGAWVMEGVGGPPAKLATRLTEAGFSAGESVGPSRIVDARVVVRGKEH
                     DVLTNATTVRELLSAMGIRPDGDDRVR"
     gene            complement(703..1497)
                     /locus_tag="VE646_05535"
     CDS             complement(703..1497)
                     /locus_tag="VE646_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZD79481.1"
                     /translation="MSEQNPELAVQSAAVDTHCHLFLLEEEPPRAVEVARAAGVGTLI
                     CVGIDPQTSRTSVELAESLPGVFATAGMHPHTASDLDEAAGAEIEELLGNPRVVGVGE
                     CGLDFFRMRSPREDQERAFRLHIGLGRESGTPLVVHVRDAWPQTLRLLEEGSADRVVL
                     HCFSGDAQVAREASARGYHLSFAANITYPKNQELRDAAAAVPMDRILIETDSPFLPPQ
                     SLRGHDNAPGNVLHVAREIAAARAMDVDDVVRATSMNARTAFPGLR"
     gene            complement(1642..3174)
                     /gene="metG"
                     /locus_tag="VE646_05540"
     CDS             complement(1642..3174)
                     /gene="metG"
                     /locus_tag="VE646_05540"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HZD79482.1"
                     /translation="MGRDVFYLTSAIPYVNAEPHVGTSYEIVGCDFVARYRRLRGDRV
                     FFLTGTDEHSINVARSAAELGIAPAEWTDRMVPKWEEVWRALRISNDDFIRTSEPRHI
                     ERVQTFVQRLYDRGEVYLGTYEGPYCVSCEEFKSEAELVDGKCSIHLKPVERLKEENY
                     FFRLSAYGDRLLQLYERDPGFVRPEVRRNEVVAFVRRGLQDLSISRSGSDWGIPVPWD
                     PTHVIYVWVDALLNYITAPGFGVDGSLFEQVWPCDLHIIGKDITRFHAIIWPAMLMAA
                     GLEVPKSVFAHGFVTVGGEKMSKSRGTGVHPSELIDHFGVDSYRYYFMREIQFGQDGN
                     WSWESMVDRHNADLANGLGNLASRVLAMLGSYFDGLVPEPGPGGAEGDLPDMIADAVQ
                     RYDGLMGEVALSPALAAVWDIVDRANQYLVEQEPWKVAKDPSRREELGGILYAAAETL
                     RILAILIQPIMPQASGRLWQQLGMAEPLDRQRLPEAAGWGLLAAGTRTEKGEPLFPRL
                     ES"
     gene            complement(3295..4089)
                     /locus_tag="VE646_05545"
     CDS             complement(3295..4089)
                     /locus_tag="VE646_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79483.1"
                     /translation="MLGSFAVLIVALAFWWSSRSPSSVEVPLPTAALPTGPSSPIAPS
                     GSPSSPVPAIGYAFLATRPDGSPYRWNPCAPIRYVVNTSGAPAGALTDVQEAARRVSS
                     ATGISFEFAGTTSETLAQRRSAGYVISTPEGSAWAPVLIAWASGPEFQALGYDPAAVG
                     AGGPFPGEAEVGQTTGRYVSGLVALNSGTNPPPAEGFSTPTSWGVVLMHDLGHVVGLA
                     HVEDPTQIMAAGLTQPRTVTDWGAGDRRGLALVGRQAGCLQAVPRS"
     gene            complement(4286..5131)
                     /gene="rsmI"
                     /locus_tag="VE646_05550"
     CDS             complement(4286..5131)
                     /gene="rsmI"
                     /locus_tag="VE646_05550"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HZD79484.1"
                     /translation="MSGTLYLVGTPIGNLGDLTERARETLGSVDLVAAEDTRRAGRLL
                     ARLGIDARTISFFEGNEERRAPQLLQRLREGERVALISDAGMPGLSDPGYRLVRGCVE
                     AGIDVRVIPGPSASVSALVLSGLPTDRFVFEGFLPRRTGDRRARLRALADLPHTLVLF
                     ESPRRVAALLRELIDELGDRRVAIARELTKRHEEVLRGTASELLEVLGERELRGETVV
                     VIEGARDRRPDVLDLDHLVKEARTLVRRGLRKRAAAAGVAHAHGGSANAIYRRLLEDE
                     RDGRD"
     gene            5178..5603
                     /locus_tag="VE646_05555"
     CDS             5178..5603
                     /locus_tag="VE646_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HZD79485.1"
                     /translation="MAAAAPHRVTYRTPLPEKLGIRDGARVLIASAPDGFAPGDLPAR
                     VQMLRAARGPLDVVLVFATRRSQLARRFGTLVHALAPAGRLWVAWPKRASGAPTDLSF
                     PVVQRMGLDAGLVDNKSAAIDQTYQGLQFVHRLVDRPRR"
     gene            5600..7153
                     /locus_tag="VE646_05560"
     CDS             5600..7153
                     /locus_tag="VE646_05560"
                     /inference="COORDINATES: protein motif:HMM:NF027518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HZD79486.1"
                     /translation="MTDTEAATAAPITQTAPGAMAALRRILNRPVVAIVAVGLIAGVL
                     RFMHLGYPGSRVFDEVYYSKSACIFLGYSNQRCDITSADEKYWRNDKNDTGAWVHPPL
                     GKWAIAAGELAFGTDAFGWRVSAAVFGTATVMVLAGMVQLLFGSPIWTFTGGLLLATE
                     SLEFVQSRVSMLDIFVTFWIASGFLLLLLDRRWMDQRSPAMAGPPEPSEPSEPHELSE
                     PSEEGAPVPGQEVAAARHVPSPFFRPWRIAAGIAFGGAVASKWSGLTGIAGALAVAFV
                     WEVSRRRRAGVRHAVWRTFQWESFPLVVIFLALPALVYVASYLGWFLHFGFHWKDWMH
                     LQGDMASYHEHLKSVDESGKPIHPYLAQAWKWIVLWRPVLYFARYGDDVRRVIYANGN
                     PAIFWGSLVALPYAGFAWWRRRDWRAGFVVLTVAALYLPWFLVSRPQFFFYATPITPF
                     FVLACVYALRDLARVHIGGSVSRPWLPVAVGLVILSVALFFWFWPVLTGGPVSNSAFQ
                     LRAWFRSWV"
     gene            7263..9263
                     /locus_tag="VE646_05565"
     CDS             7263..9263
                     /locus_tag="VE646_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HZD79487.1"
                     /translation="MAEREHTFISMGDGVRLHATLYLPQGSGPWPALLEALPYRKDDL
                     TASYRPEYERFAEHGYAVCRVDLRGTGTSEGVAEDEYPPVELRDLNEVIAWLASRRWC
                     SGAVGMFGTSYSGFNSVQVAMTRPPALRAIVSIFATDDRYTYDDVHYYGGALKALDLI
                     DYPAYMVAMNALPPVPSLYGQGWRAEWERRVTGSEPWLIRWLQEQNRSPYWDHGSLCT
                     DYGSIRAATMIVAGWADGYWKDALHVLESLRCPKRLLQGPWAHASTETSLPGPNIDLF
                     PEMVRWWDRWLKDEPNGVDRDPPIVWFARRSTRPEPDLSRMGGEWRFEPGWPLERSRR
                     HELPLSGAATPGRVGPGPDELEVRGDVGWTAWISCAGHLPYGQPVDQRPDEPFSLVYD
                     WPPLEDEVELFGRVRLDLTVSASAPVAYVSAKLCDVFPDGTSALVARGFLNLTHRHSH
                     EHPEALEPGRPVRAQIDFLLTSWVFEAGHRLRLDLAGTDWPNVWSPPQPVTITVDRSA
                     STLILPTLDGPSPLTERPALPPSGREQERPTPAPGSRGGGDERDGGVTWRIEHDVLER
                     RTRAVAGSEAWTEAEGDRPRMFEGYGGWVEVSTTDPGQARARSRTRLIVEWPEATVEA
                     EARQVVRSDAHAYHLELELDVREDGQTRWSRRWEHRFPRDLQ"
     gene            complement(9297..10043)
                     /locus_tag="VE646_05570"
     CDS             complement(9297..10043)
                     /locus_tag="VE646_05570"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZD79488.1"
                     /translation="MRLDVRARAPRPVLPARTIREELLVVLSLSLLASAVYAVISLTS
                     APVKGVTVAVVPTVPFPTQVANFVFGLAPAWLVLYLVRRNGEGTAAIGLALDRPRRDL
                     ARGLVLFLVVGAAGIAVYLGAVALGVNRFVVPVPAGHWWTVPVLFMSAIQAALLEEII
                     VAGYFITRLQQLGATSVAAILASALLRGTYHLYQGWGGFAGNFGMGLLFGFIFVRTRR
                     AWPLVIAHLLLDLGAGAGYLLFREHLPGFS"
     gene            10109..10624
                     /locus_tag="VE646_05575"
     CDS             10109..10624
                     /locus_tag="VE646_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79489.1"
                     /translation="MPAEDEFEDVAGPSEGGPEPEEAVDLAAEVAGVMEVPVGRIAVE
                     PDGDEMAWLVLDGGRVTWFNLRTTTRMKPERRSRFVRALAQLARGIDRLRPMIEGLEV
                     GHRYAVEYRNQEIRRNMRVKGVLRDLSGFRVGKGVSGAGWVLTIETKPLLGTPALFHL
                     DTDVLVDMRPA"
     gene            10669..>11518
                     /locus_tag="VE646_05580"
     CDS             10669..>11518
                     /locus_tag="VE646_05580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZD79490.1"
                     /translation="MARESADVVVVGAGTVGGWASVFAAENSARVIVLEGGLAGQGAS
                     SRAAGVVRAQGGTPATVALGRWSIDFYRSQPERLGTDSGFRELGYLILAVTGGDERAG
                     RERVAMQQRAGLDVRWVSAGEAVELNPTLAAAGHRGGSYAAGDGCIDPPRNVRAYSLA
                     MQLAGVDLRERTPFLGVRTRQRGARRRVTGVRIPGRVIATERVILTGGPSLRAVGKLV
                     GTRIPAGGARHQVAVSAPHRAFEVERQPMVFDLGAGLYWRLEEGGLLWGMSNPRERPG
                     PARDVDW"
BASE COUNT         1675 a         4084 c         4106 g         1653 t
ORIGIN      
        1 acggacgcga tcgtcaccgt cgggccggat ccccatggcc gagaggagtt cccgaaccgt
       61 ggtcgcgttg gtgagtacgt cgtgttcctt ccccctcacg acgacccgag cgtccacgat
      121 ccgggacggt ccgacggact ctcccgcgga gaaccccgcc tctgtgagtc gggtcgccag
      181 cttggccggc ggtccgccca ctccttccat cacccaggcc cccacatccc tcgaggacgg
      241 cccgaggaac ggtcgggccg acacgtccgt gtccaccacg accgtcatgc catcggccag
      301 cggcgtggcc ggcggcggct ccacgaggac cgtccccgcc agggcgatgc ccctcgcatc
      361 gaggagctcg cccacgttcg cggacagcgt gcgcacggat cgcgggtgcc cgtccaccac
      421 gagcgtgacg ttcttcacga gcgcgagata gaggaagccc gccgtcatca tcgtgacggc
      481 gagcatggcg ttgccgatcg ccttccggat ccgcaatctg cggacccggc cgggacgaac
      541 ccccatgctt cctcccagca tcgggagaag gctccggggg gaaggtccga cggggccgga
      601 ccgaagcctc catgcgcacg aaacgggacg ctatcaaacc ctcaaccctc actgcaagct
      661 tgaaagcgaa cgggtgttcg aggacccggg ccaccggctc gtctaccgga ggcccgggaa
      721 cgcggtccgt gcgttcatgg aggtcgcacg caccacgtcg tccacgtcca tcgccctcgc
      781 cgccgcgatc tctcgggcga cgtgcaggac gttgcccggg gcgttgtcgt ggccccgcag
      841 cgactgagga ggaaggaagg ggctgtcggt ctcgatcagg atccggtcca tcgggaccgc
      901 ggccgctgca tcccgcagct cctggttctt cgggtaggtg atgttcgcgg cgaacgagag
      961 gtggtagccg cgggccgagg cctcccgagc cacctgcgcg tcgccgctga agcagtggag
     1021 caccacccgg tcggccgatc cctcctccag gaggcggagg gtctggggcc acgcatcgcg
     1081 gacgtggacc accagcggtg tgcccgactc gcgaccgaga ccgatgtgga gccggaacgc
     1141 ccgctcctga tcctcccgcg gcgagcgcat ccggaagaag tccagcccgc attcgcccac
     1201 gcccaccact cgagggttcc ccaggagctc ctcgatctcc gctccggccg cctcgtccag
     1261 gtcgctcgcc gtgtgcggat gcatgccggc cgtggcgaac acgccgggca gcgactcggc
     1321 cagctctacc gacgtcctgc tggtctgcgg gtcgatcccc acgcagatca acgtgcccac
     1381 ccccgcggcc cgcgcgacct ccaccgctcg tggaggttcc tcctccaaca ggaagaggtg
     1441 gcagtgggtg tccaccgccg cgctctgcac cgcgagttca gggttctgct cgctcaccac
     1501 ggcacgacga tagcgcctct ccacctttgg tcgaccggtg gtgcaaccac cttcgagcgc
     1561 gccgaggcgg cgatcgccgt ggggtccacc cggactggag gggatgggtc tcacgtgaaa
     1621 cgtcgttccc cgaaggttca gtcacgactc gaggcgcggg aacaggggct cccccttctc
     1681 ggtgcgcgtt cccgcagcga gcaggcccca tccggcagcc tcgggcagtc gctgccgatc
     1741 caggggctcc gccatcccga gttgctgcca caggcgaccc gatgcctggg gcatgatcgg
     1801 ttggatcaag atcgccagga tccgcagcgt ctcggccgcc gcgtacagga tcccgcccag
     1861 ctcttcgcgg cggctcggat ccttggccac cttccagggc tcctgctcca ccaggtactg
     1921 gttcgcgcga tccacgatgt cccaaacggc cgccagggcc ggggagaggg ccacctcgcc
     1981 catgagcccg tcgtagcgct ggacggcgtc cgcgatcatg tcgggcaggt cgccctcggc
     2041 cccgcccgga cccggctcgg gcaccagccc atcgaagtag gagccgagca tggccagcac
     2101 ccggctggcc aggttcccca gcccgttggc cagatcggcg ttgtgccggt caaccatcga
     2161 ctcccacgac cagttgccgt cctgcccgaa ctggatctcg cgcatgaagt aatagcggta
     2221 ggagtccacc ccgaagtggt cgatcagctc ggatgggtgg acccccgtgc cacgggactt
     2281 cgacatcttc tccccgccca cggtcacgaa gccgtgagcg aagacgctct tggggacctc
     2341 caggccggcg gccatcaaca tcgccggcca gatgatcgcg tggaaccggg tgatgtcctt
     2401 gccgatgatg tgcaggtcgc acggccagac ctgctcgaag agcgacccgt ccacgccgaa
     2461 gcccggggcg gtgatgtagt tgaggagggc atccacccac acgtagatca cgtgggtggg
     2521 gtcccagggc accgggatcc cccaatccga cccgctccga gagatcgaca gatcctgaag
     2581 gcctcgccgc acgaacgcca cgacctcgtt ccttcggacc tccgggcgga cgaacccagg
     2641 atcgcgctcg tagagctgga ggagccggtc accgtacgcg gagaggcgga agaagtagtt
     2701 ctcctccttc agccgctcca ccggcttgag atggatcgaa cacttcccgt ccacgagctc
     2761 cgcctcgctc ttgaactcct cgcaggagac gcagtacggc ccctcatagg tcccgaggta
     2821 gacctcgccc cggtcgtaca gacgctgtac gaaggtctgc acgcgctcga tgtgccgggg
     2881 ctccgaggtc cggatgaaat catcgttcga gatccgcagg gccctccaga cctcctccca
     2941 cttcggcacc atccggtccg tccactccgc tggagcgatg ccgagctcgg ccgcggagcg
     3001 agccacgttg atgctgtgct cgtccgtgcc cgtgaggaag aacacccggt cgccccgcag
     3061 tcgccgatag cgcgcgacga agtcgcagcc aacgatctcg tacgacgtcc ccacgtgtgg
     3121 ctcggcgttc acgtacggga tcgccgatgt gaggtagaag acgtcccggc ccatggtcgc
     3181 gccatcctac ccggccgcgt gttggggacg ggtccctccg gacgggaacg gagcaccggc
     3241 ggactagtct ggcctggact agttgggagg tcagccgatg aggcgcgtgc ctgatcagga
     3301 ccgaggaacg gcttgcaggc accccgcctg gcggcccacc aacgcgagcc ctcgccgatc
     3361 cccggcgccc cagtcggtca ccgtccgagg ctgggtcagg cccgccgcca tgatctgcgt
     3421 gggatcttcc acgtgggcca gcccgaccac gtgccccagg tcgtgcatca gcaccacgcc
     3481 ccagctcgtg ggggtcgaga acccctccgc cggcggaggg ttggtgcccg agttcagcgc
     3541 caccaggccg ctcacgtacc gtccggtggt ctgccccacc tcggcctcgc cggggaacgg
     3601 accgccggcg cccaccgccg ccgggtcgta cccgagggcc tggaactcgg gacccgaagc
     3661 ccaagcgatc agcaccggcg cccacgccga gccctccggg gtggagatca cgtacccggc
     3721 ggatcgccgc tgcgccagcg tctcggacgt cgtgccggcg aactcgaagg agatcccggt
     3781 ggcactcgag accctgcgcg ccgcttcctg gacgtcggtc agcgcaccgg ccggggcgcc
     3841 ggacgtgttc accacgtacc ggatcggagc gcacgggttc caccggtacg gagatccgtc
     3901 gggtcgggtg gcgaggaagg cgtacccgat cgccggcacc ggcgacgagg gcgatccgct
     3961 gggagcgatc ggcgacgagg gaccggtggg gagcgcggcc gtggggagcg gcacctctac
     4021 ggaggacggg gagcgcgagc tccaccagaa ggcgagcgcc acgatcagca ccgcgaacga
     4081 tcccagcatg agcagggtgg gccaccgcgg ctccgatgtg gatcgaccgg gctgcgggga
     4141 tccgaggtcg gggccgggca ggccgtcgcg gttccctccc ggatcgtggg acggggcggc
     4201 agcgggctgg ggtcgtggtc ggggttgggc caccacggga gggagccagg tctgatcggg
     4261 cgtggctcga tgggcctcag gtcgatcagt cccgcccatc tcgctcgtcc tccagcaggc
     4321 gacggtagat cgcgttggcc gatccgccgt gcgcgtgagc caccccggcg gcggccgcac
     4381 gcttgcgcag acctcggcgg accagggtac gagcttcctt caccaggtgg tcgaggtcca
     4441 gcacgtccgg gcgccggtcc cgagcgccct cgatcaccac gaccgtctct ccacggagct
     4501 cgcgctcgcc caggacctcg agcagctccg acgcggtgcc ccggagcacc tcctcgtggc
     4561 gcttggtgag ctcgcgagcg atggcgaccc gccggtcgcc cagctcgtcg atcagctcgc
     4621 gcagcagggc cgccacccgc ctgggcgatt cgaacagcac cagggtgtgt ggcaggtcgg
     4681 ccagggcccg cagccgcgct cggcgatcgc cggtccggcg gggcaggaac ccctcgaaca
     4741 cgaaccggtc ggtcggcagc cccgagagca cgagcgccga gaccgacgcg gagggcccgg
     4801 ggatcacccg gacgtcgatc cccgcctcca cgcatccgcg caccagtcgg tacccgggat
     4861 cggacagtcc cggcatcccg gcatccgaga tcagcgcgac gcgctcgccc tcccgcaacc
     4921 gctggagcaa ctggggtgca cggcgctcct cgttgccctc gaagaaggag atcgttcggg
     4981 catcgatccc gagacgggcc agcagccgcc cggcccgccg ggtgtcctcc gcggccacca
     5041 ggtccacgga gccgagcgtc tcccgggccc gttcggtgag gtcgccgagg ttcccgatgg
     5101 gggtgccgac caggtacagg gtcccgctca cggacagatg gtcgcacggc ggggagccgc
     5161 cggtagagtg gactcccatg gccgccgcgg ctccgcaccg ggtcacgtac cgcactcccc
     5221 ttcccgagaa gctcgggatc cgggatggcg cccgggtgct gatcgccagc gccccggacg
     5281 ggttcgcgcc gggggacctg cccgcgcgcg ttcagatgct ccgagccgcc cgggggcccc
     5341 tggatgtggt cctcgtgttc gcgacgcgcc ggagccaact cgcgcgccgg ttcgggacgc
     5401 tggtccacgc cctggccccg gccggccgcc tctgggtggc ctggccgaag cgagcatcgg
     5461 gagcgcccac cgacctctcg ttcccggtgg tccagcggat gggactggat gcgggcctcg
     5521 tggacaacaa atctgcggcg atcgaccaga cgtaccaggg cctgcagttc gtccaccgcc
     5581 tcgtggaccg cccgaggcga tgaccgacac ggaagcggcc accgccgctc cgatcacaca
     5641 gacggccccg ggggccatgg ccgccctgcg ccggatcctg aaccggccgg tggtcgcgat
     5701 cgtggcggtg gggctcatcg ccggagtcct gcggttcatg cacctgggct acccgggcag
     5761 ccgcgtgttc gacgaggtct actactcgaa gtcggcctgc atcttcctcg gctactcgaa
     5821 ccagcgctgc gatatcacga gcgccgacga gaagtactgg cggaacgaca agaacgacac
     5881 gggcgcttgg gtccatcctc cgctggggaa gtgggccatc gcggcgggcg agctcgcctt
     5941 cggcaccgac gccttcggtt ggcgggtctc ggccgccgtg ttcgggacgg ccacggtgat
     6001 ggtgctggcc gggatggtgc agctcctgtt cgggagcccc atctggacct tcaccggcgg
     6061 cctgctccta gccaccgaga gcctggagtt cgtgcagtcg cgggtgtcga tgctcgacat
     6121 cttcgtcacg ttctggatcg cctcgggctt cctgctgttg ctgctcgacc ggcgatggat
     6181 ggaccagcga tcaccggcga tggcagggcc cccggagccg tccgagcctt cggagccgca
     6241 cgagctgtcg gaaccatccg aggagggcgc cccggtcccg gggcaagagg tcgccgccgc
     6301 tcggcacgtc ccctcgccct tcttccgtcc gtggcggatc gcggccggga tcgcgttcgg
     6361 gggggccgtg gcctccaagt ggtccggcct cacggggatc gccggggccc tggccgtggc
     6421 cttcgtgtgg gaggtctcgc gccggcgccg ggccggggtg cgccacgccg tctggcgcac
     6481 gttccagtgg gagagcttcc ccctggtggt catcttcctg gccctcccgg ccctcgtgta
     6541 cgtggcctcg tacctcggat ggttcctgca cttcggcttc cactggaagg actggatgca
     6601 cctgcagggg gacatggcca gctaccacga acacctcaag tcggtcgacg agagcggcaa
     6661 gccgatccac ccgtacctcg cgcaagcctg gaagtggatc gtgctgtggc gaccggtctt
     6721 gtacttcgct cggtacggcg acgacgtccg ccgcgtgatc tacgcgaacg gcaacccggc
     6781 gatcttctgg ggcagcctcg tggccctccc ctacgcgggg ttcgcctggt ggcgcaggcg
     6841 cgactggcga gccggcttcg tggtgctgac cgtggcggcc ctgtacctgc cctggttcct
     6901 cgtctcccgt ccccagttct tcttctacgc cacgccgatc acccccttct tcgtgctcgc
     6961 gtgcgtctac gcgctgcggg acctggcccg ggtgcacatc ggcgggtcgg tctcccggcc
     7021 gtggctgccg gtggccgtgg ggctggtgat cctgtcggtg gcgctgttct tctggttctg
     7081 gccggtgctg accggtggtc cggtctccaa cagcgccttc cagctccggg cctggttccg
     7141 ctcctgggtc tgaccgggtc ggagcagctc ccctcgctgc ggtggatcgc gggccgcccg
     7201 ctgaccaccg gtcacggccg tcggtgcctc ggcgcgggac gggccgacta cgatgcggcg
     7261 cgatggcgga gcgggagcac accttcatct cgatgggcga tggggtgcgc ttgcacgcca
     7321 ccctgtacct gccccagggc tccgggccgt ggccggcgct gctggaagcc ctcccctacc
     7381 gcaaggacga cctgaccgcc tcctaccgtc cggagtatga gcggttcgcg gagcacggct
     7441 acgcggtgtg ccgggtggac ctccggggga ccggcacctc cgagggtgtg gccgaggacg
     7501 agtacccgcc ggtggagctc cgagacctga acgaggtgat cgcgtggctc gcgtcgcgcc
     7561 gctggtgcag cggcgccgtg gggatgttcg ggacctcgta ctcggggttc aactcggtcc
     7621 aggtcgccat gacccggccg ccggccctca gggcgatcgt gtcgatcttc gccaccgacg
     7681 accgctacac ctacgacgac gtccattact acggcggggc gctcaaggcg ctggacctga
     7741 tcgactaccc ggcctacatg gtggcgatga acgcgctgcc gccggtcccc tccctgtacg
     7801 ggcagggctg gcgcgccgag tgggagcgac gggtcaccgg ctccgaaccc tggctgatcc
     7861 gctggctgca ggagcagaac cggagcccgt actgggacca cggctccctg tgtaccgact
     7921 acggctcgat ccgagccgcc accatgatcg tggccgggtg ggcggacggc tactggaagg
     7981 acgcgctcca cgtgctggag tccctgcgct gtcccaaacg gctgctccag gggccgtggg
     8041 cgcacgcctc gaccgagacc tcgctccccg gccccaacat cgacctgttc ccagagatgg
     8101 tgcgatggtg ggaccgctgg ttgaaggacg agccgaacgg cgtggatcgg gacccgccga
     8161 tcgtctggtt cgcgcggcgg tcgacgcggc cggagccgga cctgtcgagg atgggagggg
     8221 agtggcggtt cgagcccgga tggccgttgg agcggagccg ccggcacgag ctgcccctgt
     8281 ccggtgccgc cacccccggg cgggtaggcc ccggtcccga cgagctcgag gtgcgaggag
     8341 acgtggggtg gaccgcctgg atctcctgcg ccggacacct gccctacggg cagcccgtcg
     8401 accagcggcc cgacgagccg ttctccctgg tctacgactg gcctccgctg gaggacgagg
     8461 tggagctgtt cgggcgggtc cggctggatc tcacggtgag cgcctcggcc ccggtggcct
     8521 acgtctcggc caagctctgt gacgtcttcc ccgacggcac gtccgcgctc gtggcgcggg
     8581 ggttcctcaa cctcacccac cgccactcgc acgagcaccc ggaggcgctc gagccggggc
     8641 gaccggtccg agcccagatc gacttcctgc tcacctcgtg ggtgttcgaa gcgggccatc
     8701 gtctgcggtt ggacctggcc ggcacggact ggccgaacgt gtggagcccc ccccagcccg
     8761 tgacgatcac cgtggatcga tccgcctcca cgctgatcct gcccaccctg gatggaccgt
     8821 cccccctcac cgagcgcccg gcgctgcctc cgtccggccg cgagcaggag cgccctaccc
     8881 cggcccccgg ttcccggggt ggcggggacg aacgcgacgg aggggtcacg tggcggatcg
     8941 agcacgacgt cctcgagcgg cgcacccggg ccgtggccgg cagcgaagcc tggaccgagg
     9001 ccgaggggga ccgcccccgg atgttcgagg ggtacggcgg gtgggtggag gtctccacca
     9061 ccgaccccgg gcaggcccgg gcccggtccc ggacccggct gatcgtggag tggccggagg
     9121 ccaccgtcga ggccgaggcc cggcaggtgg tccggtccga cgcgcacgcg taccacctgg
     9181 agctcgagct ggacgtgcgg gaggacgggc agacccgctg gtcccggcgg tgggagcatc
     9241 ggttcccccg cgacctgcag tagacgggcg cctgccgctc gatcctccga cccgacctat
     9301 gagaagccgg gcaggtgctc tcggaacagc aggtagccgg ctcccgcacc cagatccagg
     9361 agcaggtggg cgatcacgag cggccatgct cttcgggtcc gcacgaagat gaagccgaag
     9421 agcaggccca tcccgaagtt gccggcgaac ccgccccagc cctgatacag gtggtaggtg
     9481 ccacgcagga gggcgctcgc gaggatcgcc gccaccgacg tggcgccgag ctgctgcagc
     9541 cgggtaatga agtagccggc cacgatgatc tcctcgagca gcgcggcctg gatcgcgctc
     9601 atgaacagca ccgggacggt ccaccagtgc cccgccggga cgggcaccac gaaccggttc
     9661 acgccgaggg ccacggcgcc caggtagacg gcgatgccgg ccgcacccac caccaggaac
     9721 agcaccagcc ctcgggccag gtcccggcga ggtcgatcca gggccaggcc gatggccgcc
     9781 gtgccctcac cgttgcggcg caccaggtac agcaccagcc aggccggtgc cagcccgaag
     9841 acgaagttcg ccacctgggt ggggaacggg accgttggga ccacggccac cgtgacgcct
     9901 ttcaccggcg ccgacgtgag gctgatgacc gcgtagacgg cgctggcgag cagggagagc
     9961 gagagcacca ccaggagctc ctcgcggatg gtcctggcgg gcaggaccgg ccgcggggct
    10021 cgggcccgca cgtccagacg catggaggaa ctgtacggcg gtcggagata ctggacccgg
    10081 agcggagcgc agaccgacgg aggcgcgcat gccggcggag gacgagttcg aggacgtcgc
    10141 gggacccagc gagggcggac ccgagccgga ggaggccgtg gatctggcgg ccgaggtggc
    10201 gggggtgatg gaggtgccgg tgggtcgcat cgccgtggag ccggatggcg acgagatggc
    10261 gtggctcgtc ctggacgggg gccgcgtcac ctggttcaac ctgcgaacga ccacccgcat
    10321 gaagcccgag cggcgttcgc gcttcgtccg ggcgctggca cagctcgccc gcggcatcga
    10381 tcgcctccga ccgatgatcg aggggctcga ggtgggccac cgctacgccg tcgagtaccg
    10441 caaccaggag atccgccgga acatgcgggt caagggggtg ctccgtgacc tatccgggtt
    10501 ccgcgtgggc aagggcgtga gcggggccgg gtgggtgctg acgatcgaga cgaagccgct
    10561 gctcgggacc ccggccctct tccacctcga caccgacgtc ctggtcgaca tgcggccggc
    10621 ctgagcgccg atagactcgc gctccggacc gacgaggagg gagcggcgat ggccagggag
    10681 tcagccgacg tcgtggtggt cggcgccggg acggtcggtg gctgggcgtc ggtcttcgcc
    10741 gccgagaaca gcgctcgggt gatcgtcctg gagggaggcc tggccggaca gggagccagc
    10801 agtcgggcgg ccggcgtggt ccgggcccag ggcggcaccc ccgccaccgt ggccctcggc
    10861 cggtggtcga tcgacttcta ccggtcgcaa cccgagcggc tgggcaccga ctccggcttc
    10921 cgcgagctcg gctacctgat cctggccgtg accggggggg acgagcgagc cggccgggag
    10981 cgggtggcca tgcagcagcg cgccgggctc gacgtgcggt gggtctcggc cggggaggcc
    11041 gtggagctga accccacgct ggccgcggcc gggcaccggg gggggagcta cgcggccggc
    11101 gacggctgca tcgatccccc caggaacgtc cgggcctact ccttggccat gcagctggcg
    11161 ggcgtcgatc tgcgcgagcg cacgccgttc ctcggcgtgc ggacccggca gcggggcgct
    11221 cggcggcggg tgaccggggt gcggatcccg ggccgggtca tcgccaccga gcgggtgatc
    11281 ctcaccggcg ggccgagcct gcgcgcggtg ggcaagctgg tcgggacgcg gatcccggcc
    11341 ggcggggcac ggcatcaggt ggccgtctcg gcgccgcacc gggccttcga ggtggaacgg
    11401 cagcccatgg tgttcgacct gggagcggga ctgtactggc ggctggagga gggcgggctg
    11461 ctgtggggca tgagcaatcc gagggaacgg ccgggacccg ctcgcgacgt ggactggc
//
LOCUS       DASQQQ010000133         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_393785, whole genome shotgun sequence.
ACCESSION   DASQQQ010000133 DASQQQ010000000
VERSION     DASQQQ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393785"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VE646_05585"
     CDS             <1..395
                     /locus_tag="VE646_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HZD79491.1"
                     /translation="PFEPGPVVDQFGWRFRVVEVDGGRVVQAFWRGGWEDHYTFTLEP
                     QFPVDYEVANHFTSTHPDSRFVRTLTAQLSWPERSLILRDLELSEVTPDGGTVRQIAA
                     GELLDVLASRFALSFPEGTRFRTRSERG"
     gene            complement(457..972)
                     /locus_tag="VE646_05590"
     CDS             complement(457..972)
                     /locus_tag="VE646_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZD79492.1"
                     /translation="METWDTIRARRNVRRYADRPIAEDDLQRIVEAARRTPSSTNQQG
                     WDLVVVTDRDRLRRLSGAWRYARHLADSAATIVLVMPAVEDRDERDTYWYDLGQMTMS
                     IMLAAADLGIGSSHASIAEQELVRETLGLPEDREAAIFIALGYPADRALRPIEHPKRR
                     ALEDILHRDRW"
     gene            complement(1016..2194)
                     /locus_tag="VE646_05595"
     CDS             complement(1016..2194)
                     /locus_tag="VE646_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HZD79493.1"
                     /translation="MARIGIEQRRARLGFRHRLGEPAATVDEVARAMVGLHSSDPSTV
                     FLSAYARVQRFRTADMEDALYERRSLVRMLGMRRTLFVVPRDLAAVMDEACTKALAPG
                     ERRRLTRMLEEQGVAPGSGGARWIRRVEHATLRALGARGEATARELTKDVPELGSKLT
                     FGEGKRWGGTMGVSTRILFLLATDGRIVRTRPLGGWTSGQYRWARTENWLAEPLPVVE
                     HDEARTDLLRRWLRAFGPGTMADVRWWTGWTAKLAAQTLRAVEAVEVEMKEGTGYLLP
                     DDLEPVPRPEPWAALLPGLDPTVMGWKERDWFLGPHGRLLFDRNGNAGPTVWADGRLV
                     GGWTQSADGEVVVRLLEPLDRTTRRRIEAERERLRAWLGAVRIRPRFRSPIEQELSGP
                     "
BASE COUNT          318 a          881 c          799 g          341 t
ORIGIN      
        1 tgcccttcga gccggggccg gtcgtggacc agttcggatg gcggttccgt gtggtggagg
       61 tggacggggg ccgagtggtc caggcgttct ggcggggcgg gtgggaggac cactacacgt
      121 tcacgctcga gccccagttc ccggtcgact acgaggtcgc caaccacttc acgtccacgc
      181 atccggactc ccggttcgtc cggacgctca ccgcccagct ctcctggccc gagcgctcac
      241 tgatcctgcg ggatctcgag ctctccgagg tcacgccgga cggagggacc gtccgccaga
      301 tcgccgcagg ggagctcctg gacgtgctgg catcgcggtt cgcgctgagc ttcccggaag
      361 ggacccggtt ccgaacccgg tccgagcgcg ggtagggcga ccgtccgatc ggggcggcgg
      421 gaagggctgg cgagcatcgc tggctaccag gactcgctac cagcggtccc ggtggaggat
      481 gtcctccagc gcccggcgct tgggatgctc gatcggccgc agcgcacgat cggccgggta
      541 ccccagggcg atgaagatcg ccgcctcccg gtcctcgggc aggcccagcg tctcccggac
      601 cagctcctgc tcggcgatcg aggcgtggct gctcccgatc ccgaggtccg ccgcggccag
      661 catgatcgac atcgtcatct gacccaggtc gtaccagtag gtgtcgcgct cgtcccggtc
      721 ctcgacggcc ggcatgacca ggacgatcgt ggcggccgag tcggccaggt ggcgcgcgta
      781 ccgccacgcc cccgacagcc ggcggagccg atcgcggtcg gtcaccacca ccaggtccca
      841 gccctgctgg ttcgtggatg agggcgtgcg ccgggccgcc tcgacgatcc gctggaggtc
      901 gtcctcggcg atcggccggt ccgcgtaccg ccggacgttc cgacgagccc ggatcgtgtc
      961 ccaggtctcc atcccccaac cttacccgtc ggagcgggaa cggcggcccg gccgctcagg
     1021 gtcccgagag ctcttgctcg atcggtgaac ggaagcgggg ccggatccgg accgctccca
     1081 gccaggcgcg caggcgttcg cgctcggcct cgatccgccg tcgggtggtc cggtcgaggg
     1141 gctcgaggag ccggacgacc acctcgccgt cggcgctctg cgtccatccc ccgaccagcc
     1201 gcccatccgc ccagaccgtg ggcccggcgt tgccgttgcg gtcgaacagg aggcgcccgt
     1261 ggggccccag gaaccagtcg cgctccttcc accccatcac ggtggggtcg agtccgggca
     1321 gcagcgcggc ccacggttcg ggccgcggca cgggctcgag gtcgtcgggc aggaggtacc
     1381 cggtgccctc cttcatctcg acctcgacgg cctccaccgc ccgcagggtc tgtgcggcga
     1441 gcttcgccgt ccacccggtc caccaccgga cgtccgccat cgtgccggga ccgaatgccc
     1501 tgagccatcg acgcaacagg tcggtgcggg cctcatcgtg ctccaccaca gggagcggtt
     1561 cggcgagcca gttctcggtg cgcgcccacc ggtactggcc cgaggtccat cctcccagcg
     1621 gccgggtccg gacgatcctc ccgtccgtgg ccagcaggaa gaggatcctg gtggagaccc
     1681 ccatcgtgcc gccccagcgc ttgccttccc cgaacgtcag cttgctcccg agctcgggca
     1741 cgtccttcgt gagctcccgt gcggtggcct cgccccgagc accgagcgct cggagcgtcg
     1801 cgtgctccac ccgacggatc caccgtgctc ccccggatcc gggcgcgacg ccctgttcct
     1861 cgagcatccg ggtcaggcgc cgacgctcgc ccggtgccag cgccttggtg cacgcctcgt
     1921 ccatcacggc ggccaggtcg cggggcacca cgaacaacgt tcggcgcatc cccaacatcc
     1981 ggaccagcga tcgtcgctcg tagagggcgt cttccatgtc ggcagtccgg aagcgctgca
     2041 cccgcgcgta cgccgagagg aacacggtgg agggatcgct cgagtgcagg cccaccatcg
     2101 cccgggcgac ctcgtccacc gtggccgccg gctcccccag gcggtgccgg aacccgagcc
     2161 gagcgcgccg ctgctcgatg ccgatgcgtg ccatgaccaa ggggagggtc aagcctcttc
     2221 ccgcgcccgg acgatggaca catccctcac gaggtcaagg agatgtccac gggtcccgat
     2281 cctcgatcgc ccgcgaggaa cgagccgagg cggctgccct cctccgcgat ctcggcccg
//
LOCUS       DASQQQ010000134         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_204921, whole genome shotgun sequence.
ACCESSION   DASQQQ010000134 DASQQQ010000000
VERSION     DASQQQ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204921"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..819
                     /locus_tag="VE646_05600"
     CDS             49..819
                     /locus_tag="VE646_05600"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HZD79494.1"
                     /translation="MPSVARINITPVKSTTLHHPPCVELGPRGAEGDRRFFLVDGDGR
                     RFSGDRKGPLLAVRADHDPATDRLRFRLPEGAEAEGPAAATGDALVVNFYGRDVPAHL
                     VPGPWEGMLSAFVGRPVRLARPDRTGDALDVEPVTLVSLASVDELSRQGGSEGRVDPD
                     RFRMLLEVDGCGPHEEDRWWGRLLKIGRAVIRAGAPVPRCVVTTLDPATGMRDFPTLS
                     VIKRYRGVTSGNELPFGIYGRVERVGAVAVGDRIDLLD"
     gene            complement(828..1703)
                     /locus_tag="VE646_05605"
     CDS             complement(828..1703)
                     /locus_tag="VE646_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD79495.1"
                     /translation="MGREAIALAEVTVTVPATVGETLQHDLRGIAVVWRREMIRFFRN
                     RMRIVTSLVQPILFLFVLGTGLARLIPGGAGFDFRTFMFPGILAMTVLFTAIFSAVSI
                     VWDREFGFLREMLVAPIGRTALVMGKILGGGTVATVQAAIMLALAGLVRIPYSPTMLL
                     ALLGMMALTAFTVTAVGTLVASRMTQIESFQVVIQFIVLPMFFLSGAVFPVSQLPAWL
                     EALTRVDPLSYAVDPMRGAIFGHIGVAPKVAAILNPGMTWAGWHVATWLELLVMALIG
                     VVSLIGAAVQFSRTE"
     gene            complement(1685..>2281)
                     /locus_tag="VE646_05610"
     CDS             complement(1685..>2281)
                     /locus_tag="VE646_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HZD79496.1"
                     /translation="RDGLVRTFSGGMKRRLEIARGLLHSPRVLFLDEPTIGLDPQTRA
                     HIWEYVVQLKAREGITLFMTTHYMDEAEYCDRIAIIDEGRIVVTGTPEELKASVGADR
                     VEIRTADDDAAVAVLGRRFGIEARHADGLVRFQVQQGGEFVPRLFAELEIPIAAVSVA
                     RPSLDDVYMTYTGRTIRDAEASITDRNRSRRQRWAGRR"
BASE COUNT          361 a          813 c          775 g          332 t
ORIGIN      
        1 acgctatcac cgccgctccc gcgcccggcc actggtagcg tggacgccat gccttccgtg
       61 gcccggatca acatcacccc ggtcaagtcc accaccttgc atcacccgcc gtgcgtggag
      121 ctgggccccc ggggggccga gggcgaccgg cggttcttcc tggtcgacgg cgatggccgg
      181 cgcttctccg gcgaccgcaa ggggccgctg ctcgccgtcc gggccgatca cgacccggcg
      241 acggacaggc tccgattccg tctccccgag ggtgcggagg cggaggggcc ggccgccgcc
      301 accggggatg cgctcgtggt gaacttctat gggcgtgacg tgcccgccca tctggtgccc
      361 ggaccgtggg aggggatgct ctcggcgttc gtcggtcggc cggtccgctt ggcccggccc
      421 gaccggacgg gcgatgccct cgacgtggaa ccggtcaccc tggtctccct ggcctcggtg
      481 gatgagctct cgcgccaggg cggcagcgag ggtcgcgtgg atcccgaccg gttccgcatg
      541 ctgctcgagg tcgacggctg cggcccccac gaggaggacc ggtggtgggg acggctgctc
      601 aagatcggca gggccgtgat ccgggccggc gcacccgtgc cgcggtgcgt ggtgaccacg
      661 ctggacccag ccacggggat gcgggacttc cccacgctct cggtgatcaa gcgctaccgg
      721 ggcgtgacct ccggcaacga gcttccgttc gggatctacg gccgggtgga acgggtgggc
      781 gccgtggctg tgggtgaccg gatcgatctc ctggactgac cctgacctca ctcggtgcgg
      841 gagaactgga cggccgcacc gatcagggag accacgccga tcagggccat gaccagcagt
      901 tccagccacg tggccacatg ccaaccggcc cacgtcatgc ccgggttcag gatcgcggcc
      961 accttcggcg ccaccccgat gtgcccgaag atcgcccccc gcatcggatc caccgcgtag
     1021 ctcagagggt cgacgcgcgt cagggcctcc agccaggcgg gcagctgcga gacggggaac
     1081 accgcaccgg acaagaagaa catcggcagg acgatgaact ggatcacgac ctggaacgat
     1141 tcgatctggg tcatccggct ggcgaccaac gtcccgaccg ccgtcaccgt gaaggccgtg
     1201 agcgccatca tgcccagcag cgccaggagc atcgtcggcg agtaggggat ccttaccagc
     1261 ccggccaacg cgagcatgat ggccgcctgg accgtggcga cggtgccgcc cccgaggatc
     1321 ttccccatga ccagggcggt ccggccgatc ggcgccacca gcatctcccg caggaagccg
     1381 aactcccgat cccacacgat cgacacggcg gagaagatgg ccgtgaacag gacggtcatg
     1441 gccaggatgc ctgggaacat gaacgttcgg aagtcgaagc ccgcaccgcc cgggatcagc
     1501 ctggccagtc ccgtccccag gacgaacagg aacaggatcg gctgcacgag ggaggtcacg
     1561 atccgcatcc tgttgcggaa gaagcggatc atctctcgcc gccagaccac cgcgatcccc
     1621 cgcaggtcgt gctgcagggt ctcgccgacg gtggcgggaa cggtgacggt cacttcggcc
     1681 agggctatcg cctccctgcc caccgttgcc ggcgggatcg gttgcgatcg gtgatggacg
     1741 cctcggcgtc ccggatggtc cgccccgtgt aggtcatgta cacatcgtcc aggctcggtc
     1801 gtgccacgct caccgcggcg atggggatct ccagctcggc gaacaggcgc ggcacgaact
     1861 ctccgccctg ctgcacctgg aaccgcacca ggccgtcggc atgcctggct tcgatgccga
     1921 accgccgtcc caacacggcg acagccgcat cgtcgtccgc ggtgcggatc tccacccggt
     1981 cggccccgac gctggccttc agctcctccg gcgtgccggt gaccacgatc cgaccctcgt
     2041 cgatgatcgc gatccgatcg cagtactccg cctcgtccat gtagtgggtc gtcatgaaca
     2101 gcgtgatgcc ctcgcgggcc ttgagctgca ccacgtactc ccagatgtgg gcccgggtct
     2161 gggggtcgag gccgatcgtc ggctcgtcca ggaacaggac gcgcggcgaa tgcagcagcc
     2221 cgcgagcgat ctccagccgc cgcttcatgc cgccggagaa cgtgcgcacc aggccgtccc
     2281 g
//
LOCUS       DASQQQ010000135         5626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_120245, whole genome shotgun sequence.
ACCESSION   DASQQQ010000135 DASQQQ010000000
VERSION     DASQQQ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120245"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..614)
                     /locus_tag="VE646_05615"
     CDS             complement(204..614)
                     /locus_tag="VE646_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79497.1"
                     /translation="MDVDAERPARPSDAPDRPGTAPGRRSDRWAVGARGARNPWAWTS
                     AGAALAGASAVAAIAAATAGIAPLDRLPDRWAYALFAAIPSAGVVAVAAGLTGRARFR
                     LRWLCWIGAVLGALLVIGAIAVVIVLSLSLRKLG"
     gene            complement(673..1200)
                     /locus_tag="VE646_05620"
     CDS             complement(673..1200)
                     /locus_tag="VE646_05620"
                     /inference="COORDINATES: protein motif:HMM:NF036411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5319 family protein"
                     /protein_id="HZD79498.1"
                     /translation="MEEPLDRRGNEDEPEPTRLEPEEEENVRADLEDLDGMRDVFAQQ
                     GVKGVVIACPDCGENHFYEWDLLKENLEHMLQTGEPRMHEPAFEVREDEYIQWDYGKG
                     YIDALGDTGLEPGRRVSVTRCPWCTTPAQEYFQYCPRCGRSLAALRIYKDLVERGMDE
                     RDVRALLVRAGFEPF"
     gene            complement(1200..1901)
                     /locus_tag="VE646_05625"
     CDS             complement(1200..1901)
                     /locus_tag="VE646_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HZD79499.1"
                     /translation="MRFATALVLFAHPDDAEYMCGGTVALWAREGTEVHYVCITDGSA
                     GNNEPGAVREEIAKVRKQEQRDAAAVLGVKTVTFLDFADGMLEVTLDARRAVAREVRR
                     IRPDVLVAPDPARLWSAARSYINHADHRAAGELALCAVMPDAPSRPQFPELLDDGFEP
                     YEIPSLWLGAEEPDTYIDITKTIDVKLEALTRHRSQQGEASAPWVRERGRQLGEAGGV
                     EYAEGFRTFVLKEEE"
     gene            1951..2322
                     /locus_tag="VE646_05630"
     CDS             1951..2322
                     /locus_tag="VE646_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79500.1"
                     /translation="MDHEGPERGSGLREEVPRAGALGTATALGGLWGLLGYTVLWDGT
                     PFATDRRFVESVLGTMALLPIRLVLWGIRAFELLTGRTYNFPDSNWWIGAVAGLLGAL
                     IVAGGVLAIRTTARWVRSLGA"
     gene            complement(2319..3653)
                     /locus_tag="VE646_05635"
     CDS             complement(2319..3653)
                     /locus_tag="VE646_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZD79501.1"
                     /translation="MTRRVERPGPPLSGLRALAAFRRDPLGVLIDAARYGDLVRIDLP
                     RYPAHLVNHPDLIREVLVADGSRFEKGPTMQAARRTLGQGLLTSEGEVHKRQRRMIQP
                     IFHHDRIDGYGEAMVEEAVRVTDGWSDGRVLDLRDEMAQLTLAIVARTLFGARVGTTQ
                     AREIGDALALQLTMFDRVFSPLFVITDRLPLPSNRRFDRAKAAIDAVVERMIARRRQE
                     DTAGNDLLSLLIGAQEDGQGMSDRQVRDEAITLFLAGHETTANALTWTWHLLSAYPEA
                     EERLHAEVDEALGDRPPTVGDLPRLAYTRMVLTESMRLLPPAWAIGRRATVPLELGDR
                     RLPRGAVVVVSPYLVHRDPRWYRDPEAFAPERWSEDRSAAPRLAYLPFGAGSRMCIGE
                     PFAWLEGVLVLATLARAWRFRTVPGHPIELQPVITLRPRFGVVATAHRRRRG"
     gene            complement(3650..4018)
                     /locus_tag="VE646_05640"
     CDS             complement(3650..4018)
                     /locus_tag="VE646_05640"
                     /inference="COORDINATES: protein motif:HMM:NF018008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZD79502.1"
                     /translation="MSEGVDRSIRVIKADRHHDLHWDEVERPEGDTNPPGEEVIVFRS
                     GDQRFSFGMWQRVPEEGPMEPPYHEIAVLIEGEVELTEEDGTVHRAGPGDILITPEGS
                     RATWKALSPVKKFWAVYKEP"
     gene            complement(4052..4531)
                     /locus_tag="VE646_05645"
     CDS             complement(4052..4531)
                     /locus_tag="VE646_05645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZD79503.1"
                     /translation="MEEQGAIRPAHLVAVRDLRQRVLRPHQRAEELVYPGDEAPDSLH
                     LGAFRDDELLGIATIVREAPPGTSDPTAWRVRGMATLPEVRGKGYGAALLEACLDHAS
                     SRGGGLVWCNARATAVEFYRRFGFEVRGEPFTLPVIGAHLFMWRPLEPRAAPDPPGG"
     gene            complement(4543..>5626)
                     /gene="argH"
                     /locus_tag="VE646_05650"
     CDS             complement(4543..>5626)
                     /gene="argH"
                     /locus_tag="VE646_05650"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HZD79504.1"
                     /translation="LHAGRSRNDLVVTDLRLWLLDAGREIAAGLERLVRALLGHARAH
                     AETVMPGTTHGRLAQPVTLGHHLAAHGWALLRDLERLDGWRERSSVSALGAGALAATS
                     LPLDPAAIAERLGLRPFGNSIDAVSDRDFGQEFLATGAICGTHLSRLAADLIRWSDEA
                     LGWAELDEAFTTGSSMMPQKRNPDVAELSRAKAGRVLGDFTTLTSVLNGLPLGYQRDL
                     QEDKEPLFDAADTLRLVTAALAGCVETVRFHADAMRRAVGAEELFATDLAEVLVKGGV
                     PFREAHRRVGVLLRELSEQGRSLRDLTEEEWLQHGLPDGAELLDPDRSVRARTLRGGP
                     SPEQVLAQVRAIEDALGPGAEGTDRR"
BASE COUNT          782 a         2109 c         1941 g          794 t
ORIGIN      
        1 tgtgcgtggt ggagttcctc cagatcaccc ggagcacgaa gaagatgcgc gcgccgtcct
       61 tcaaggggct gcgcgaggac aagctgccgg gggagtgctt cctggagctc cctgctccgg
      121 gctcgcccgg cgccccaccg acctccttat cgcggacgcg gcgtcgttga ggcggcacac
      181 ggacggcttg accgcgggat cgttcagccc agcttgcgga gcgagaggga gaggacgatc
      241 acgacggcga tcgcaccgat caccaacagg gcgcccagca ccgccccgat ccagcacaac
      301 cagcgcagcc ggaaccgagc tctgcccgtg aggccggccg cgacggccac gaccccggcc
      361 gacgggatcg ccgcgaacag cgcataggcc cagcggtcgg gcaggcgatc cagcggggcg
      421 atccccgccg tggccgcggc gatggccgct accgccgagg cgccggccag cgcggcgccg
      481 gcggacgtcc aggcccaggg gttcctcgcc ccgcgcgctc cgacggccca tcggtcgctc
      541 cgccgcccgg gcgccgttcc ggggcggtcg ggcgcatcgg agggacgagc cgggcgctcg
      601 gcgtcgacgt ccatcgcgcc tacctaccat cccacggagg ccgactcaag cgcccccagc
      661 ctggtccgcg tctcagaagg gctcgaagcc ggcgcggacc agcagggccc gcacgtcgcg
      721 ctcgtccatg ccgcgctcca ccaggtcctt gtagatccgc agcgccgcga gcgatcgacc
      781 gcacctgggg cagtactgga agtactcctg cgccggcgtg gtgcaccacg ggcaccgtgt
      841 gacggacacc cgccgccccg gctcgaggcc ggtgtcgccg agtgcgtcga tgtacccctt
      901 gccgtagtcc cactggatgt actcgtcctc gcgcacctcg aacgccggct cgtgcatccg
      961 cggctcaccg gtctggagca tgtgctcgag gttctccttc agcaggtccc actcgtagaa
     1021 gtggttctcc ccacagtctg ggcaggcgat cacgacgccc ttgaccccct gctgggcgaa
     1081 gacgtcccgc atgccgtcca ggtcctcgag gtcggcgcgg acgttctcct cctcctccgg
     1141 ctccagacgc gtgggctcgg gctcgtcttc gttgccccgg cgatcgagcg gctcctccat
     1201 cactcctctt ccttcaagac gaacgtgcgg aacccctccg cgtactccac gccaccggct
     1261 tcccccagct gccggccgcg ttcgcggacc cacggggccg atgcctcacc ttgctgagaa
     1321 cggtggcggg tgagggcttc cagcttcacg tcgatcgtct tcgtgatgtc gatgtacgta
     1381 tcgggctcct cggcgccgag ccaaaggctg gggatctcgt agggctcgaa gccgtcgtcc
     1441 aggagctcgg ggaactgcgg acgggacggc gcgtcgggca tcacggcgca cagcgccagc
     1501 tcgcccgccg cgcggtgatc cgcgtggttg atgtagctac gcgcggccga ccacaggcga
     1561 gcggggtccg gagccaccag cacgtcgggg cggatccggc ggacctcccg ggctacggct
     1621 cggcgtgcgt ccagggtcac ctcgagcatc ccgtcggcga agtcgaggaa cgtcacggtc
     1681 ttcaccccca ggaccgccgc ggcgtcccgc tgctcctgct tgcggacctt cgcgatctct
     1741 tcccggacgg cccccggctc gttgttgccg gccgagccgt cggtgatgca gacgtagtgg
     1801 acctccgtgc cctcgcgagc ccacagggcg accgtgcccc cgcacatgta ctcggcgtcg
     1861 tcggggtggg cgaagaggac cagcgcggtt gcgaacctca tgccatcgag cctaccggac
     1921 ggatcccgga acctgcagca gacttggggc atggaccacg agggaccgga gcgggggtct
     1981 gggctgaggg aagaggtccc ccgtgccggc gcgctcggga ccgcgaccgc gctgggcggg
     2041 ctctggggcc tgctgggcta cacggtgctc tgggacggca cgcccttcgc gaccgatcgc
     2101 cggttcgtgg agtcggtgct gggcaccatg gccctgctgc ccatccggct cgtgctctgg
     2161 gggatccggg cattcgagct cctcaccggc cggacctaca acttcccgga ctcgaactgg
     2221 tggatcggtg cggtggccgg tctcctcggt gccctgatcg tggcgggagg cgtcctggcg
     2281 atccggacca cggcgcgctg ggtgcgctcg ctcggcgcct agccgcggcg ccggcggtgc
     2341 gccgtcgcca ccacgccgaa cctcggtcgg agcgtgatga cgggctgcag ctcgatgggg
     2401 tgccccggca ccgtccggaa ccgccaggcc cgggccaggg tggccagcac caacaccccc
     2461 tcgagccagg cgaacggttc tccgatgcac atccgcgacc cggccccgaa tgggaggtac
     2521 gcgagccgag gcgccgcgct ccggtcctcg ctccaccgct cgggagcgaa ggcctcggga
     2581 tcccggtacc agcgggggtc ccggtgcacc aggtagggag acaccaccac gacggctccc
     2641 cgagggagcc gccggtctcc cagctcgagt gggaccgtgg cccgccggcc gatcgcccag
     2701 gccggcggca ggagccgcat cgactcggtc aggaccatcc gcgtgtaggc caggcgtggc
     2761 aggtccccca cggtcggcgg gcgatcgccg agcgcctcgt cgacctccgc gtgcaggcgc
     2821 tcctccgctt ccgggtaggc cgacaacagg tgccacgtcc aggtcagcgc gttggcggtg
     2881 gtctcatggc cggccaggaa cagggtgatc gcctcgtccc ggacctgccg atcgctcatc
     2941 ccctgcccat cctcctgagc cccgatcagg agcgagagca ggtcgttacc ggccgtgtcc
     3001 tcctggcgtc gacgggcgat catccgctcc accacggcgt cgatcgcggc cttggcccga
     3061 tcgaaccggc ggttgctggg cagcggcagc cggtcggtga tgacgaacag gggcgagaac
     3121 acccggtcga acatcgtcag ctgaagcgcc agcgcgtcac cgatctcccg agcctgggtg
     3181 gtccccaccc gcgcgccgaa cagggtccga gcgacgatgg cgagcgtgag ctgcgccatc
     3241 tcgtcgcgga ggtccaggac ccggccgtcg ctccacccgt cggtgacccg aaccgcctcc
     3301 tccaccatcg cctcgccgta gccgtcgatc cggtcgtggt ggaagatggg ctggatcatc
     3361 ctccgctgac gcttgtgcac ctccccctcg ctggtcagga gcccttggcc cagggtccgc
     3421 cgggccgcct gcatggtggg tcccttctcg aaccgggacc cgtcggcaac cagcacctcg
     3481 cggatcagat cggggtggtt caccaggtgg gcggggtacc ggggcaggtc gatgcggacg
     3541 aggtcgccgt agcgtgccgc gtcgatcagc acccccaggg ggtctcgtcg gaacgccgcg
     3601 agcgcacgca gcccggacag cggcgggccc ggccgctcca cccgccgggt cacggctcct
     3661 tgtacacggc ccagaacttc ttcaccgggg agagggcctt ccaggtcgcc ctggatccct
     3721 ccggcgtgat gaggatgtca ccggggccgg cccgatggac cgtgccgtcc tcctcggtca
     3781 gctccacctc accctcgatc aacaccgcga tctcgtggta cgggggctcc atcggtccct
     3841 cctcggggac ccgctgccac atcccgaagc tgaaccgctg gtcccccgat cggaagacga
     3901 tgacctcttc acccggaggg ttcgtgtcgc cctccgggcg ctccacctcg tcccaatgca
     3961 ggtcgtggtg ccggtccgcc ttgatcaccc ggatgctgcg gtcgaccccc tcgctcatcg
     4021 ccagacctcc gacgtcggct gcggggacag gctacccacc cggcgggtcg ggcgccgctc
     4081 ggggctcgag gggacgccac atgaagaggt gggcaccgat cacggggagg gtgaagggct
     4141 cgcctcggac ctcgaacccg aagcgccggt agaactccac cgccgtggct ctggcgttgc
     4201 accagaccag cccgccgccc cgggacgatg cgtgatcgag gcaggcttcc agcagggccg
     4261 cgccgtaccc cttgccccgg acctcgggga gcgtggccat gccccgtaca cgccatgcgg
     4321 tcgggtccga ggtccccggg ggggcctcgc gcacgatcgt ggcgatgccc agcagctcgt
     4381 cgtcccggaa ggccccgagg tgcaggctgt ccggcgcctc gtcgcccggg tacacgagct
     4441 cctcggcccg ctggtgcggc cgcagcaccc gttgacgcag atcccggacc gcgaccaggt
     4501 gggccggccg gatcgcgccc tgctcctcca tgtcagacga gcctagcgtc gatcggtgcc
     4561 ctcggctccc gggccgaggg catcctcgat cgctcgcacc tgcgccaaga cctgctcggg
     4621 ggaggggcca ccccgcaggg tccgagcgcg taccgaccgg tccggatcga ggagctcggc
     4681 cccgtcgggg agcccgtgtt gcagccactc ctcctcggta aggtcgcgca gggaccgtcc
     4741 ctgctccgac agctcccgca gcaggacgcc gacccggcgg tgggcctcgc ggaacggcac
     4801 cccgcccttc accaacacct cggccaggtc cgtggcgaac agctcctcgg cccccacggc
     4861 ccggcgcatc gcgtcggcgt ggaaccggac cgtctccacg cagccggcga gcgccgccgt
     4921 caccaaccgc agcgtgtcgg cggcgtcgaa cagcggctcc ttgtcctcct ggagatcccg
     4981 ctggtacccg agggggagcc cgttcaggac cgaggtgagg gtggtgaagt ctcccagcac
     5041 ccgaccggcc ttggcccggg acagctcggc gacgtccggg ttccgcttct gcggcatcat
     5101 gctggagccg gtcgtgaacg cctcgtccag ctccgcccag cccaaggcct cgtcgctcca
     5161 ccggatcagg tccgccgcca accgggacag gtgggtgccg cagatcgcgc ctgtcgccag
     5221 gaactcctgc ccgaagtccc ggtccgagac ggcatcgatc gagttgccga agggccggag
     5281 tcccaggcgc tcggcgatgg cggccgggtc caggggcagg gaggtcgcgg ccagcgcccc
     5341 ggcccccagc gccgacacgg acgagcgctc ccgccatcca tccaggcgct ccagatcccg
     5401 caggagcgcc cacccgtggg cggccaggtg gtgaccgagg gtgaccggct gggccaggcg
     5461 cccgtgcgtg gtcccgggca tgaccgtctc ggcgtgtgcc cgggcgtggc cgaggagcgc
     5521 ccgcaccagg cgctccaggc ccgcggcgat ctcccgaccg gcatcgagca gccacagccg
     5581 caggtccgtc accaccagat cgttacggct ccgacccgcg tggagc
//
LOCUS       DASQQQ010000136         3397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_66744, whole genome shotgun sequence.
ACCESSION   DASQQQ010000136 DASQQQ010000000
VERSION     DASQQQ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66744"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VE646_05655"
     CDS             complement(<1..230)
                     /locus_tag="VE646_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZD79505.1"
                     /translation="MAQTRYDAIVIGGGHNGLVAAAYLAKAGRRVVVLEKRDRVGGIL
                     DTVDVAPGVRAPGVAHTVGRLRTSVVRDLGLRR"
     gene            complement(230..1834)
                     /locus_tag="VE646_05660"
     CDS             complement(230..1834)
                     /locus_tag="VE646_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZD79506.1"
                     /translation="MARRYDAVVIGGGHNGLTAAAYLARAGRRVLVLERRHVLGGAAV
                     TEEVFPGFRFSEFSYVVSLLRPEIIRELNLPAHGLEILPLDGTITPLEDDALWRVNDH
                     GKTIRELRRWSKSDAEAYEEYGQLMVDMARFIKPILSVVPPDPGVMNPFDWTPLVPLA
                     KSFRDLSPRLQSTFLQLMTMSATDFLRQWFETDPLIATMSASGIIGTYQGIQSPGTAY
                     VLLHHYMGEIDGAFRAWGVPKGGTGGISEAIGRAAQAAGADIRTEAPVARIDVRNGRA
                     TGVTLGSGEEIEAGVVLSSLDSNWTFVKLVEPGHLDEDFLREVRRYKYRGSSGKVNLA
                     LDGLPELACKPGVGEWLRGAISFSPGVDYMERAYDEAKYGMFSSRPYVDMIIPTLVDP
                     SMAPPGKHIVSCFVQYAPYHLADGEVWDDQMRERFGETVIDTLEERFPKIRDHILHKQ
                     FLTPLDIEEMTGLTEGNIFQGELSLEQLFFNRPVPGWAKYRTPIRDLWICGSAAHPGG
                     GIMGAPGRLAALEVLKTQKAGRRAVA"
     gene            2059..3249
                     /locus_tag="VE646_05665"
     CDS             2059..3249
                     /locus_tag="VE646_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HZD79507.1"
                     /translation="MSVGTAFHPRTSQLNQKMAWGEWSGYFSAQVYADFHDIEYNAIR
                     EAAALIDVTPLYKYAISGPDATRLVDRVMTRDASKLAIDQVMYTPWCDERGKVIDDGT
                     VARRKEREYFWTAADASYRWFVLNAHGLDVQIEDVTDTVAGLAVQGPKSRDLLEVLTG
                     QDWKDLRYFRHRRTEIAGIDVNVTRTGYTGDLGYELWVDAGAALELWDAVWEGGEAFG
                     IRAAGIHALDVTRVEAGLILAEVEYTSARHAISAEQEYSPFEISLGRLVDFGKADFVG
                     KHALELEQVSGGPARRLVGLELDWSGIETMFAKHDQPPAVSAFVHRDPVPVYRQGKRV
                     GRATSVTWGPTIKKMVGFGSLPPSLASPGTRLSVEWTVEGERGRVGASVDETPFLDLP
                     RKRA"
BASE COUNT          514 a         1204 c         1172 g          507 t
ORIGIN      
        1 cgccgcaggc cgaggtcccg gaccaccgag gtccgcaagc gccccacggt gtgcgccaca
       61 ccgggtgccc ggacgccagg cgccacgtcc acggtgtcga ggatcccgcc cacccggtcc
      121 cgcttctcca gcaccaccac gcgtcgaccc gccttggcca ggtacgccgc cgccaccagg
      181 ccgttgtgcc cgccaccgat cacgatcgcg tcgtaccggg tctgcgccat cacgccaccg
      241 ccctccggcc ggccttctgc gtcttcagca cctccagggc ggccagccgc ccgggcgcgc
      301 ccatgatgcc gccgcccgga tgcgcggccg agccgcagat ccacaggtcc ctgatggggg
      361 tccggtactt cgcccacccg ggcaccggac ggttgaagaa cagctgctcg agggagagct
      421 cgccctggaa gatgttgcct tcggtcaggc cggtcatctc ctcgatgtcg agcggcgtga
      481 ggaactgctt gtgcaggatg tgatcccgga tcttcgggaa ccgctcctcc agcgtgtcga
      541 tcacggtctc accgaaccgc tcgcgcatct ggtcgtccca gacctcgccg tcggccaggt
      601 ggtacggcgc gtactgcacg aagcagctca cgatgtgctt gccgggcggg gccatcgagg
      661 ggtcgaccag cgtggggatg atcatgtcga cgtacggccg ggagctgaac atcccgtact
      721 tcgcctcgtc gtaggcgcgc tccatgtagt ccacgcccgg cgagaagctg atcgcgccac
      781 ggagccattc acccaccccg ggcttgcacg ccagctcggg caggccgtcc agcgccaggt
      841 tcaccttgcc ggaggagccg cggtacttgt agcgcctcac ctcgcgcagg aagtcctcgt
      901 ccaggtggcc gggctccacc agcttcacga acgtccagtt cgagtccagg ctcgagagca
      961 ccacgccggc ctcgatctcc tccccggacc cgagcgtgac gccggtggcc cgaccgttgc
     1021 gcacgtcgat ccgggcgacc ggtgcttccg tccggatgtc ggctccggcg gcctgggccg
     1081 cccgtccgat cgcctcggag atcccgccgg tgccgccctt ggggacaccc caggcccgga
     1141 aggcaccgtc gatctcgccc atgtagtggt gcagcagcac gtacgcggtc ccgggcgact
     1201 ggatcccctg gtaggtcccg atgatgcccg aggcggacat ggtggcgatc agcggatcgg
     1261 tctcgaacca ctgccgcagg aaatcggtgg cgctcatcgt catcagctgc aggaacgtgg
     1321 actgcagacg aggggacaga tcgcggaagc tcttggccag cggcacgagc ggcgtccagt
     1381 cgaacgggtt catgacgccc gggtccggcg gcaccaccga caggatcggc ttgatgaacc
     1441 gcgccatgtc gaccatcagc tggccgtact cctcgtaggc ctcggcgtcg ctcttggacc
     1501 agcggcgcag ctcccggatc gtcttgccat ggtcgttcac ccgccacagg gcgtcgtcct
     1561 cgaggggcgt gatcgtcccg tccagcggca ggatctccag gccgtgggcg ggcaggttca
     1621 gctcgcggat gatctcgggc cggagcaacg agaccacgta ggagaactcg gagaaccgga
     1681 acccggggaa gacctcttcg gtgacggcgg ccccgcccag cacgtggcgc cgctccagga
     1741 ccaggacccg acggccggcc cgggccaggt aggccgccgc cgtgagcccg ttgtgtccgc
     1801 cgccgatcac gaccgcgtcg tagcgccgtg ccaccgttcc ctccttccat cccgtggggg
     1861 acccgtgacg gggaccgacg cggtcctcgc gccgaaccca gcgcgtcagc gccgacgcca
     1921 tgctaggccg ggcaaccccc gccgaccagc gaccccttga ccacaggtca gcgcgggagg
     1981 cgctggggac ggtcggggcc gaggcgttgc gtgcatactc tgagcggaca cccacccgcg
     2041 gtcggagagg agggcgtcgt gagcgtcggc accgcgttcc acccgcgaac ctcgcagctc
     2101 aaccagaaga tggcctgggg ggagtggtcc ggctacttct ccgcacaggt ctacgcggac
     2161 ttccacgaca tcgagtacaa cgcgatccgc gaggcggccg cgctgatcga cgtgaccccg
     2221 ctgtacaagt acgccatctc cggccccgac gccactcggc tggtggaccg cgtgatgacc
     2281 cgcgacgcat ccaagctggc gatcgatcag gtgatgtaca cgccgtggtg cgacgagcgc
     2341 ggcaaggtga tcgacgacgg caccgtcgcc cgccgtaagg agcgcgagta cttctggacg
     2401 gcggccgacg ccagctaccg gtggttcgtg ctgaacgcgc acggtctgga cgtgcagatc
     2461 gaggatgtca ccgatacggt tgccgggctg gccgtccagg gccccaagtc ccgcgacctc
     2521 ctcgaggtcc tcaccggtca ggactggaag gacctgaggt acttccgcca ccgccgcacg
     2581 gagatcgccg ggatcgacgt gaacgtgacc cggaccggct acaccggcga tctcggctac
     2641 gagctgtggg tggacgccgg ggccgcactg gagctgtggg atgccgtgtg ggaggggggc
     2701 gaggccttcg ggatccgcgc ggccggcatc cacgccctcg acgtcacccg cgtggaagcc
     2761 gggctgatcc tggccgaggt cgagtacacg agcgcccggc acgcgatcag cgccgagcag
     2821 gagtactccc ccttcgagat ctcgctgggc aggctggtcg acttcgggaa ggccgacttc
     2881 gtgggtaagc acgccctgga gctcgagcag gtgtcgggcg gaccggcgcg acgcctggtg
     2941 ggcctggaac tcgattggag cgggatcgag acgatgttcg cgaagcacga tcagccaccc
     3001 gcggtctcgg ccttcgtgca ccgcgacccc gtcccggtct accggcaggg caagcgggtg
     3061 ggacgagcca ccagcgtcac ctggggaccg accatcaaga agatggtggg cttcggctcc
     3121 ctgccgccct cgctcgcttc tcccgggacg cgcctgtccg tggagtggac ggtcgagggc
     3181 gaacgcggcc gggtcggtgc ctcggtggac gagacgccgt tcctggacct gccccgcaag
     3241 cgcgcctgag gggccgaacc cggccccgta tgcgatgatg ggggcccgtg cgcgtttgtt
     3301 cggcccgcgt cccgatcctc ctgatgctcg cttgcggtgt ggccgcgctg gccgtggcct
     3361 tccggagcgg tttggtggcg gccggcgtgg cgggcat
//
LOCUS       DASQQQ010000137         7085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_311069, whole genome shotgun sequence.
ACCESSION   DASQQQ010000137 DASQQQ010000000
VERSION     DASQQQ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7085
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311069"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VE646_05670"
     CDS             complement(<1..222)
                     /locus_tag="VE646_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD79508.1"
                     /translation="MSNKDRFVVGSDQLRVLVVDDHALFRRGLQMVLDQEPDLALVGE
                     ASDGAEAVEKAQELMPDVILMDVRMPKRSG"
     gene            complement(234..605)
                     /gene="raiA"
                     /locus_tag="VE646_05675"
     CDS             complement(234..605)
                     /gene="raiA"
                     /locus_tag="VE646_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HZD79509.1"
                     /translation="MDLVLKGRGTRITEHMRGAAEHKLARLGRMAPRVTRIEIEVISE
                     KNPRQGGVKRVEAALETPRKTFRAVAMGSDVDSALDQLAEKLERQLRDHREKRRTRLV
                     GGGKRLKSAQAETTSQEGSEE"
     gene            complement(843..1709)
                     /locus_tag="VE646_05680"
     CDS             complement(843..1709)
                     /locus_tag="VE646_05680"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HZD79510.1"
                     /translation="MSSGSSCVATAVAPRAAWSFPCPDRPLGAACPARCRIPSLGSRH
                     ARPLLDVLYPRRCAACGSGPWPFCPGCRDLLVPMEPPWCERCGLPATRSLRRCSDCPP
                     AAIDLARSAFLFRGPARSAVHRLKFSGWRAVAGALGQACVAVWSEPANVATWVPLSRA
                     RLTARRFDQARALAEVVAAGLGRPVEGFLVRAQDTAPQARRGATDRRTALAEAFHALR
                     PPGHQRVLLVDDVLTTGATAAACADALRTAGATWVGLLTAARAGSPRTRGGYTRAGLT
                     PGSVVARGAFPR"
     gene            complement(1639..2283)
                     /locus_tag="VE646_05685"
     CDS             complement(1639..2283)
                     /locus_tag="VE646_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79511.1"
                     /translation="MSDRISVRTRFERFPATLKGAFILRGEDRDPHQVILQAARTESL
                     DGRVQRALPVGEATLDIVPRRDLFVPFEVSVSDLDPGWYELVCDLRVDGVSATYRGDR
                     RFAVAWPRASVRRGRVEVGERFPLGPSEVRIGDVECTGDSIRVELSVTPPSPVTARLS
                     ADGARHEILSMDLDQASGVGRIVAYPLLKVHRELRIELRGHGRGAEGGLVVPLP"
     gene            complement(2280..3338)
                     /gene="mtnA"
                     /locus_tag="VE646_05690"
     CDS             complement(2280..3338)
                     /gene="mtnA"
                     /locus_tag="VE646_05690"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HZD79512.1"
                     /translation="MSGVPSPVEWRGDLVAILDQRALPLREGYLECRSVEDVAEAIRS
                     LAVRGAPVLGITAAFGMALAVTTSTARGTNGLFRELNRAGDLLKATRPTAVNIGWAVD
                     RMLETARGECCSVGSTRQRVHEALVAEALTIEREDREACAAMGRAGAELVPDPADVLT
                     HCNTGSLCTAGIGTAQGVIVAAHGAGRRVHVWVDETRPVWQGARLTAWELQRLGIPMT
                     LVADGAAGSLMAAGKVDLVVVGADRIAANGDVANKVGTYGLAVLAAHHGIPFYVAAPL
                     STIDRSTPTGADIAIEERDAAEVTAPLGGQVAPPGTPAANPAFDVTPAELITAIVTDA
                     GVLRPPYAETLARVRGTG"
     gene            complement(3335..3727)
                     /locus_tag="VE646_05695"
     CDS             complement(3335..3727)
                     /locus_tag="VE646_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZD79513.1"
                     /translation="MGAGDLAGFREAGRTLFSLGLIKGSEGNLSTWDGERLLITRTGC
                     ELARLSDEDVLRGSLDDPPANASSDLAIHLRWYRERGAGAVAHAHPAGTVPPGWGEGQ
                     AHGSYAFGSDLAAAVAQIVGSVRAERDT"
     gene            complement(3709..4965)
                     /gene="ahcY"
                     /locus_tag="VE646_05700"
     CDS             complement(3709..4965)
                     /gene="ahcY"
                     /locus_tag="VE646_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HZD79514.1"
                     /translation="MDCDVRDPGLAGKGRERIEWAAGEMRVLAQIRERFEKEKPLQGV
                     RVGACLHVTTETANLMETLKAGGADIALCASNPLSTQDDVAAALCEQSGIRAFAIKGE
                     DHDTYYRHISAVLDHHPQITMDDGADLVSLLHKERRDQVDEVIGGTEETTTGVIRLRA
                     MAADGALRYPIVSVNDANTKHLFDNRFGTGQSTMDAIMRSTNLLLAGRTVVVCGYGMC
                     GRGVASRARGLGARVVVTEVDPLPALEAAMEGYQVMSLREAARLGDVFVTVTGDTSVI
                     RGEHMELMKDGAVMANSGHFDVEIDKVALAGMAVEQRRIREFVDEYRLADGRRLHLLG
                     EGRLVNLAAAEGHPAAVMDMSFANQALTAEWVVKSRGTLTPGVHSVPAEIDAEVARLK
                     LRAMGIEIDRLTPEQEEYLSSWEQGT"
     gene            complement(4950..5792)
                     /locus_tag="VE646_05705"
     CDS             complement(4950..5792)
                     /locus_tag="VE646_05705"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HZD79515.1"
                     /translation="MIAALPGPGDSLADDAAEAVRGRTGFEPAVCIVLGSGLGAAVGS
                     LREEASFGFGELPGFPRPTVPGHAGRLVLGELGGVPVAAFLGRIHYYEGNDMNVCALP
                     VRLARLLGAGTAILTAATGGIGEGLTTGHLVVGMDHLNLMGVSPLRGWRSPDGSPPFV
                     DMVGAYDRDLADLAMASASDHGVPVSRGVYAAMSGPSFETPAEIEMLRRSGASVVGMS
                     VVPEAVPARAMGMRLLGLFFVSNEVGVPVSHEEVIKASDAMAAALGEVIRDVLMGGAG
                     WTAT"
     gene            complement(5789..6847)
                     /locus_tag="VE646_05710"
     CDS             complement(5789..6847)
                     /locus_tag="VE646_05710"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HZD79516.1"
                     /translation="MIDLDDEVAIRASDPGNMIDVVAGLPHHCLEGHRLGLGASPPPV
                     AEGITALAFCGMGGSAVAGDVIAALGAPHLPVPVQVVRSPVLPAYCRTDTLVLVSSYS
                     GNTAEALGCFEEAVRRGCRLAVLTSGGRLAARAHELGLPRVPLPEGFQPRAALGYLAL
                     GSLGLLEGLGWLPPMGEDLRLAAGDLTTLAATLGPSVPTSVNPAKRLAVGIGDRVPVI
                     WGAEGIGAVAADRWRTQMNENGKVAAWSSALPELDHNEVVGWSEDQGERFCVIALRHE
                     GDHPDVADRFDLSGQIARSAGARTEEVWARDSSMLGRFLSLVMVGDFTSVYVGVRRGI
                     DPTPIDAIARLKRALSAT"
     gene            complement(6844..7032)
                     /locus_tag="VE646_05715"
     CDS             complement(6844..7032)
                     /locus_tag="VE646_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HZD79517.1"
                     /translation="MSLDPQLLEILVCPNDRGEVDYLEAEQVIECRECGYRYPVRDGI
                     PVMLIDEAEKPRGKKKRR"
BASE COUNT         1002 a         2775 c         2364 g          944 t
ORIGIN      
        1 cccggagcgc ttgggcatgc ggacgtccat caggatcacg tccggcatga gctcctgcgc
       61 cttctccacg gcctcggcgc cgtcggaggc ctcgcccacg agcgcgaggt ccggctcctg
      121 gtccaggacc atctgcaggc cccgacggaa gagcgcgtga tcgtccacca ccaacacccg
      181 gagctggtcc gatccgacga cgaagcgatc cttgttcgac acgacccctc cggctactcc
      241 tccgacccct cctgcgaggt ggtctccgcc tgggccgatt ttagtcgctt gccgcccccc
      301 accaagcgcg tgcggcgttt ctctcggtga tcgcggagct gccgctccag cttctcggcc
      361 agctgatcca gggccgagtc cacgtcgctg cccatcgcca ccgcccggaa ggtcttgcgg
      421 ggggtctcca gggcggcctc cacgcgcttc acgccgccct ggcgggggtt cttctcgctg
      481 atgacctcga tctcgatgcg ggtcacgcgc ggggccatac gcccgagcct ggcgagcttg
      541 tgctcggcgg cgccgcgcat gtgctcggtg atcctggtgc cgcgtccctt caggaccaga
      601 tccatcggcg ttccttcctc cgtctccctc acgcgctgcg cccccggttc cggggggagg
      661 tgggctcacg cccggctgac ctggtctttg cccccacact cgcctgctga ggggatcgcc
      721 gcccgaggcg gcttagcacc ttctcccggc acggaccggg gcaggactta catctaaacc
      781 gcaccatgct caacgaccaa gggtcgcctt acgtgggtcg cttcgcctgc ggctggcatc
      841 gactaccggg ggaacgcccc ccgggcaacc acagacccag gcgtgagccc cgcgcgagta
      901 tacccaccgc gggtgcgggg gctgccggcc cgggccgccg tcagcagccc cacccaggtg
      961 gcgccggccg tgcgcagcgc gtcggcgcag gcggcggccg tggcgccggt ggtgaggacg
     1021 tcgtccacca gcagcacccg ctgatgccca ggaggtcgca gggcgtggaa ggcctccgcc
     1081 agcgcggtgc gccggtcggt ggcgccgcgc cgggcctgcg gcgcggtgtc ctgggcccgg
     1141 accaggaacc cctccacggg acgacccagg cccgcggcga cgacctccgc cagggcccgt
     1201 gcctggtcga agcggcgagc ggtcaggcgg gcgcgggaca gcggcaccca ggtggccaca
     1261 ttggcgggct cggaccacac ggcgacgcag gcctggccta acgcgcccgc caccgcccgc
     1321 caccccgaga acttcagccg gtgcacggcc gagcgggcgg ggccccggaa caggaacgcg
     1381 gagcgggcca ggtcgatcgc cgccggcggg cagtccgaac agcgccgcaa gctgcgggtc
     1441 gcgggtagcc cgcaccgctc gcaccagggc ggctccatcg gcaccaggag gtctcggcag
     1501 ccgggacaga acggccaggg cccggacccg caggcggcgc accggcgcgg atacaggacg
     1561 tccagcaggg gccgagcatg ccgcgaccct agcgagggga tgcgacatcg agccgggcac
     1621 gcggccccaa ggggtcggtc agggcagggg aacgaccagg ccgccctcgg cgccacggcc
     1681 gtggccacgc agctcgatcc ggagctcacg atggaccttg agcagcgggt aggccacgat
     1741 ccgacccacg cccgaggcct ggtccagatc catcgagagg atctcgtgac gggcgccgtc
     1801 ggcgctgaga cgggcggtga ccgggctcgg cggggtgacg gacagctcga cccggatcga
     1861 gtccccggtg cactccacgt ctccgatccg cacctccgag ggaccgagcg ggaaccgctc
     1921 tcccacctcg acccgtcccc ggcggaccga ggcccggggc cacgccaccg cgaacctgcg
     1981 gtcgccgcgg tacgtggccg agaccccgtc cacccgaaga tcgcacacca gctcgtacca
     2041 gcctgggtcc aggtccgaga cgctcacctc gaacggcacg aacaggtccc gcctggggac
     2101 gatgtcgagg gttgcctcgc ccaccggcag ggcccgctgg acccgaccgt ccagggactc
     2161 ggtcctggcc gcctggagga tcacctggtg aggatcgcga tcctcgcccc ggaggatgaa
     2221 ggcgcccttg agcgtggccg ggaagcgctc gaaccgcgtg cgcaccgaga tccgatcgct
     2281 caccccgtcc cccgaaccct cgccaacgtc tcagcgtacg gcggccggag cacgcccgca
     2341 tcggtgacga tggccgtgat cagctcggcc ggggtcacgt cgaaggccgg gttggcggcg
     2401 ggggtcccgg gcggggccac ctgcccgccc agcggtgcgg tcacctccgc ggcgtctcgc
     2461 tcctcgatcg cgatgtccgc gccggtgggc gtgctccggt cgatcgtgga caggggcgcc
     2521 gcgacgtaga acgggatccc gtggtgtgcg gccagcaccg cgagcccgta cgtgcccacc
     2581 ttgttcgcca cgtcgccgtt cgcggcgatc cgatcggccc ccaccaccac caggtccacc
     2641 ttgcccgccg ccatcaggga tccggcagcc ccgtcggcca ccagggtcat cgggatcccc
     2701 agccgctgga gctcccacgc ggtcagccgg gccccctgcc ataccggccg ggtctcgtcc
     2761 acccagacgt gcacccgccg cccggccccg tgggcggcga cgatgacgcc ctgcgccgtg
     2821 ccgatcccgg ccgtgcacag gctcccggtg ttgcagtggg tgaggacgtc ggccggatcg
     2881 gggacgagct cggctccggc ccggcccatg gccgcgcagg cctcgcgatc ctcacgctcg
     2941 atcgtgaggg cctccgcgac caacgcctcg tggacccgtt ggcgggtgct gccgaccgag
     3001 cagcactcgc cccgagccgt ctccagcatc cggtcgaccg cccacccgat gttcacggcg
     3061 gtgggtcggg tggccttcag caggtccccc gcccggttca gctcgcggaa gagaccgttc
     3121 gtgcctctgg ccgtggacgt cgtcacggcc agcgccatcc cgaacgccgc cgtgatcccg
     3181 agcaccggcg ctccccggac cgccagggag cggatcgcct cggccacgtc ctccacgctc
     3241 cggcactcca ggtacccctc gcggagcggg agcgcccgct ggtcgaggat cgcgacgagg
     3301 tcgccgcgcc actccacggg tgacgggacc ccgctcacgt gtcccgctcc gcccggaccg
     3361 aaccgacgat ctgcgccacg gccgccgcca ggtccgaccc gaacgcgtag ctgccgtggg
     3421 cctgtccctc cccccatccg ggcggcaccg ttcccgcggg atgggcgtgg gccaccgcgc
     3481 cggccccgcg ctcccggtac cagcgcaggt ggatggccag gtccgaggag gcgttcgccg
     3541 gcgggtcgtc cagcgaaccc ctcagcacgt cctcatccga cagccgggcc agctcgcacc
     3601 cggtgcgggt gatcagcagg cgctcgccgt cccaggtgga caggttgccc tcgctgccct
     3661 tgatcagccc gagcgagaac agggtccgcc cggcctcccg gaaccccgct aggtcccctg
     3721 ctcccacgag ctcaggtact cctcctgctc cggcgtgagg cggtcgatct cgatccccat
     3781 ggcccggagc ttcagcctgg ccacctcggc gtcgatctcg gccggcacgc tgtggacccc
     3841 gggggtcagg gtgccccgcg acttgaccac ccactccgcc gtcaacgcct ggttggcgaa
     3901 ggacatgtcc atgaccgcgg ccggatgccc ctcggccgcg gccaggttca ccaacctgcc
     3961 ctcgcccaac aggtgcaacc ggcggccatc ggccagacga tactcgtcca cgaactcccg
     4021 gatccgccgc tgctccacgg ccatcccggc cagggccacc ttgtcgatct cgacgtcgaa
     4081 gtgacccgag ttcgccatca cggcgccgtc cttcatcagc tccatgtgct ccccgcggat
     4141 cacgctggtg tccccggtca ccgtgacgaa cacgtccccg agccgcgccg cctcccggag
     4201 ggacatcacc tggtagccct ccatcgccgc ttccagcgcg gggagcgggt ccacctcggt
     4261 caccaccacg cgagcaccca gaccccgcgc ccgggaggcc acgccccgcc cgcacatgcc
     4321 gtacccgcac acgaccaccg tgcggccggc caggagcagg ttcgtagatc gcatgatcgc
     4381 gtccatcgtg gactgcccgg tgccgaaccg gttgtcgaac aggtgcttgg tgttggcgtc
     4441 gttcaccgag acgatcggat agcggagcgc cccgtcggcg gccatggccc gcaggcggat
     4501 caccccggtg gtggtctcct cggtgccgcc gatcacctca tcgacctggt cccgacgctc
     4561 cttgtggagc aggctcacca ggtcggcgcc gtcgtccatc gtgatctggg gatggtgatc
     4621 gagtacggcg ctgatgtgcc ggtagtacgt gtcgtggtcc tcgcccttga tggcgaaggc
     4681 gcggatcccg ctctgctcgc acagggcggc ggcgacgtcg tcctgcgtgg agagtgggtt
     4741 cgaggcgcac agggcgatgt cggccccccc ggccttcagc gtctccatca ggttcgccgt
     4801 ctcggtggtg acgtgcaggc acgcccccac ccgcaccccc tggagcggct tctccttctc
     4861 gaaccgctcg cggatctggg ccagcacacg catctctccg gccgcccact cgatccgctc
     4921 ccgccccttc ccggccaggc ctgggtccct cacgtcgcag tccatcccgc tcctcccatc
     4981 agcacgtcac ggatcacctc accgagggcg gccgccatgg cgtccgaggc cttgatgacc
     5041 tcctcgtggc tcacggggac cccgacctcg ttcgagacga agaacagccc cagcagccgc
     5101 atccccatgg cccgggcggg tacggcctcg ggcaccacgg acatccccac caccgacgcc
     5161 cccgaccggc gcagcatctc gatctcggcc ggggtctcga agctcgggcc ggacatggcc
     5221 gcgtacacgc cccgcgacac ggggacaccg tgatcggagg ccgaggccat cgcgaggtcc
     5281 gccagatcgc ggtcgtaggc cccgaccatg tccacgaacg gcggagaccc gtccgggctg
     5341 cgccagccac gtagggggct cacccccatc aggttcaggt ggtccatccc caccaccagg
     5401 tgcccggtgg tgaggccctc cccgataccc ccggtcgccg cggtgaggat cgccgtcccc
     5461 gcacccagca gacgggccaa ccggaccggc agcgcgcaca cgttcatgtc gttgccttcg
     5521 taatagtgga tccgacccag gaaggccgcc accgggacgc cgcccagctc gcccagcacc
     5581 aggcgcccgg cgtgaccggg cacggtgggg cgagggaagc cgggcagctc cccgaacccg
     5641 aacgatgcct cctcgcggag cgatcccacc gcggccccca ggcccgaccc cagcacgata
     5701 cacacggcgg gttcgaagcc ggtccgcccc cgcacggcct cggcggcgtc gtccgcgagc
     5761 gagtctccgg ggccagggag cgcggcgatc acgtcgccga cagcgcccgt ttcagccggg
     5821 cgatggcgtc gatgggtgtg ggatcgatcc cccgtcgcac ccccacgtac acggacgtga
     5881 agtcccccac catgacgagc gacaggaacc ggcccagcat cgaggagtcg cgggcccaca
     5941 cctcctcggt ccgcgccccg gcggagcggg cgatctgacc ggacaggtcg aaccggtccg
     6001 ccacgtccgg atggtccccc tcgtggcgca gggcgatcac gcagaaccgc tcgccctggt
     6061 cctcggacca ccccacgacc tcgttgtgat cgagctcggg cagggccgag gaccaggcag
     6121 cgaccttccc gttctcgttc atctgggtcc gccaccggtc ggccgctacg gcgccgatcc
     6181 cctcggcccc ccagatgacc ggcacccggt ccccgatccc gacggccagc cgcttggccg
     6241 ggttcaccga ggtgggaacc gagggcccca gcgtggcggc gagcgtggtg aggtcgccgg
     6301 cggccaggcg gagatcctcc cccatcggcg ggagccagcc caagccctcg agcagcccga
     6361 gcgagcccag cgccaggtag ccgagagccg cgcggggctg gaagccctcg ggcaggggga
     6421 cccgtggcag accgagctcg tgggcccggg ccgccagccg cccccccgag gtcaggacgg
     6481 ccagccggca gccccgccgc acggcctcct cgaagcatcc cagcgcctcg gccgtgttcc
     6541 ccgagtacga ggacacgagg accagggtgt cggtccggca gtaggcgggc agcaccgggc
     6601 tccggaccac ctgcacgggc actggcagat ggggcgctcc cagggcggcg atcacgtccc
     6661 cggccacggc cgatccgccc atcccgcaga aggcgagtgc cgtgatgccc tcggccactg
     6721 ggggcggcga ggcacccagg cccagccggt gcccctccag gcagtggtgg ggcaggccgg
     6781 ccacgacgtc gatcatgttc cccgggtcgc tcgcccggat cgcgacctcg tcgtcgaggt
     6841 cgatcaccgg cgcttcttct tcccccgggg cttctccgcc tcgtcgatca acatgaccgg
     6901 gatgccgtcg cgcacgggat accgataccc gcactcccgg cactcgatca cctgctcggc
     6961 ctccaggtag tccacctcgc ctcggtcgtt ggggcagacc aggatctcga gcagctgcgg
     7021 gtccaggctc acgcgtcacc tcctcgccct ctgatcaggg ccagcacctc atccgtcttc
     7081 tgctg
//
LOCUS       DASQQQ010000138         8343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_66811, whole genome shotgun sequence.
ACCESSION   DASQQQ010000138 DASQQQ010000000
VERSION     DASQQQ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8343
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66811"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VE646_05720"
     CDS             <1..274
                     /locus_tag="VE646_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79518.1"
                     /translation="EETSSPKVTAQDAGVAPDFSGCAGKTGLDNAICRHEVLVGIHPD
                     NPGLENSLTRLHDNLDRQQERAKATSHGPSGDHGASGDHGNPGGNH"
     gene            complement(329..1030)
                     /locus_tag="VE646_05725"
     CDS             complement(329..1030)
                     /locus_tag="VE646_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HZD79519.1"
                     /translation="MRVVIAGAGAVGRHLGSDLAARKHQVTVIEQVPEILREAREWDP
                     DLEYLLGDACEPWVLEKADLKTADVVVAATGDDEDNLVISLLSKQEFAVPRVLARVNH
                     PRNEWMFNEQWGVDVAVSVPHLLTALVEEAVTVGDLVRLLRLEGGRVGLLELTIPPSS
                     PNAGRPLYELRLPTDSAIVAVLREGHVVIPQPETVIAAGDEILALSSKDSEPTLREAI
                     VGEGVPGSSPVDVDD"
     gene            complement(1032..1691)
                     /locus_tag="VE646_05730"
     CDS             complement(1032..1691)
                     /locus_tag="VE646_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HZD79520.1"
                     /translation="MHAIIVGCGRVGSELTIQLGRAGHEVAIIDKRPSAFDRLPPDFK
                     ARTIVGLGFDRDILEEAGIKDADAFIAVTNGDNSNIVSARVAREHYHVPKVIARIYDP
                     RRAEIYERLNIPTVATTTWGVKQVMLMLAHPREEIKESLAGGDLFRMRLQVPEHLVGT
                     PIRELNLDGKTCVVGVDRGGHGFIPVADSTFQEGDFAALIVHKDALAELDELLTPVAE
                     Q"
     gene            1824..4151
                     /locus_tag="VE646_05735"
     CDS             1824..4151
                     /locus_tag="VE646_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZD79521.1"
                     /translation="MAPNGTASPVDVLKRLLVGRALASHKQEHQLLPKILALPVFSSD
                     PLSSVAYATEEMMLVLVAAGAGALSLKLPIAVGIALLLAIVVTSYRQTVRAYPQGGGS
                     YIVARENLGTVPGLVAAAAILTDYVLTVAVSVTAGTIAITSAFPSLVGDRVPIAIALV
                     GLVTMVNLRGVRESGIVFAVPTYGFVALVMVTLAAGFVECATGCPRAATADLPLQAEA
                     GLGLFLGLKAFSSGATALTGVEAIADGVPAFRRPQSHNAATTLAMMGVISITMFLGIT
                     ALAEFLRVRVTAELAASRSVLSQIGETVFGRNALFFLLQVFTAGILVLAANTSFQDFP
                     RLSSILARDRFMPSQFRNRGDRLVFSNGVLVLALLASALIWVFHAQLTHLIQLYVVGV
                     FTAFTLSQAGMVRRWMRRRGPQWRRSAIINGIGASVTGVVLVIVTITKFRGGAWIVIT
                     AIPVIVAFFLAVHHHYERVGTMLRARDLAVDAERRNAVVFVVADLGPATIDAVAYLRA
                     LRPERVEPVFVGPADAYERVATAWDHRAPRFGRLHPLRQAQLHPVRALRDHVRAMART
                     QEEFVTVLIPELLHSHTWRQFLRRRQTLLLKAAFLFEPDIVVMNVPLLPEELLDAEAH
                     RHHTAELERTVALVPVSAVHDATVEAVSYAISLNPWRAEALFLATDLNEVPAIIEQWG
                     NKQMPIPLSAIEAPFRDIGAPLLEEVRKHTDRGDTVVTVVIPEFVVHRWWEHLLHNQT
                     AFYIKRLLLFEPRVVVASVPFHLGAESRAATAAAS"
     gene            4164..6497
                     /locus_tag="VE646_05740"
     CDS             4164..6497
                     /locus_tag="VE646_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZD79522.1"
                     /translation="MSELSSRRIQGRVGGYLKRVLVGRAVASQKLEHTLLPKILALPV
                     FSSDALSSVAYATEEILTVLLGIGVGAVAYPKVLPISVAIALLMAIVVTSYRQTVRAY
                     PNGGGAYIVSKDNLGTVPGLVAAAALLVDYVLTVSVSIVAGVFALVSAAPALNQHKVI
                     LSLVFIAFITLANLRGVRESGTLFAVPTYAFILSILSMIVVGLIGCVGGCPAAQPVTP
                     LPELAGATAASIPLFQILRAFASGSTALTGVEAISNGVPAFRRPQARNAGETLAIMAT
                     IAITMFLGISFLASHMHVTVSADRSVVAQIAHTAFGGGFAFYMVQTFTTAILVLAANT
                     SFQDFPRLSAILARDRFMPRQFQNRGDRLVFSNGVIVLAGLAGLLVYAFDANLSRLIQ
                     LYVVGVFTSFTLSQTGMVRHWLKERHRGPEAQRGWQRAIVINAIGAVATFIVLIVVLM
                     TKFEHGAWIVVVAIPVLVVLFMTVHRHYVSVMTQLRRGTVRAGEIGVNHLVLLVRELD
                     AATAEAVGVVRSIRPTELHVVHPTSNGIAPELQQRWREFSMGAPDLEVLPVSNGNLTQ
                     AVRAYIGRLDRASSDFVNVVIPEVIGEGLAGYLFRRWSLVRLKAGLLREPNVVVTDVP
                     VHVDEGNLGRVDGRPLIPSRTVALAFVSAVNDASIRAVNYAQSLEAAETRAIYFDLDP
                     DVAHRMQEEWGAAGLRIPLDIVEAPFRDLTGPMLDEVRRHTEREDTVVLVVVPEFVVR
                     RWRQMVLHNQNALFVKRLFLFEPRVVLSSVPFPLETR"
     gene            6497..7048
                     /locus_tag="VE646_05745"
     CDS             6497..7048
                     /locus_tag="VE646_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HZD79523.1"
                     /translation="MDRDQALALCREKAEKETTVRHLISVEGVMRALGRRFGEDQDLW
                     GLTGLFHDLDQDETHDDPERHAFLAAGWLREAGVDERIVNGVLAHAREEHRTDLMSKA
                     VVHADAVAGLLVAAALVRPERAVGMKVSSVKKKLKERAFAPGVNRAEVTGVDGSIGLT
                     LDEFLAVSIEGLQLVAPEIGLVP"
     gene            complement(7062..7943)
                     /locus_tag="VE646_05750"
     CDS             complement(7062..7943)
                     /locus_tag="VE646_05750"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZD79524.1"
                     /translation="MDLSGWGPIRRGRALWEQVQRIRAVDLVRAVGHRSGENNGGQMA
                     AALTYYAFLSLFPLLLLALSVIGYLLKDDPTAQREWVNRLAGSVPGLGSIIASNMRAV
                     VEGRATAGAIGLAGLLWAGMGMVGGARTTLARIFRLPRASNPVLKRFRTVGALAALGV
                     VALGSVSVTGFLAGLGSEAGVGLAVRALGIAGAAAVDLGFFLLAYRVLAPGSGPSYPY
                     LVPGALLMAAGWTGLKLAGAWYAARVISRASAIYGTFAAVIGVLAIFNIAAQLFVYGA
                     ELCAVMSERRHERTGVL"
     gene            complement(8030..>8343)
                     /locus_tag="VE646_05755"
     CDS             complement(8030..>8343)
                     /locus_tag="VE646_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014092866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZD79525.1"
                     /translation="PVVRTFISLPAGVARMNVWRFSVYTLLGCLPWTFGLTILGYQLG
                     ERWDRVEQVIRPFSWAFGAVLAVGIGWWVWRRVRELRVRPLPSREDDPGEEPEREPVG
                     R"
BASE COUNT         1168 a         2923 c         2920 g         1332 t
ORIGIN      
        1 ggaagagaca tcgagcccca aggtgaccgc ccaggacgcg ggggtcgcac ccgacttctc
       61 cgggtgcgcc ggcaagaccg gcctggacaa cgcgatctgc cggcacgagg tgctggtggg
      121 gatccacccg gacaaccccg ggctggagaa ctcgctgacc cgcctgcacg acaacctgga
      181 ccggcagcaa gagagggcca aggcgacgtc gcacggcccc tccggggacc acggtgcgtc
      241 gggcgaccac gggaaccccg gcgggaacca ctaggacgtc gggccggcct ggggaaggcg
      301 cgccgaacca agtccgatgc ccgcggacct agtcgtccac gtcgaccggg gatgagcccg
      361 ggacgccctc cccgacgatc gcctcccgca gggtgggctc ggagtccttc gaggataggg
      421 ccaggatctc gtcgcccgcc gcgatcaccg tctcgggctg cgggatgacc acgtggccct
      481 cgcggagcac ggcgacgatc gccgagtccg tgggcagacg cagctcgtag agtggccgtc
      541 ccgcgttcgg cgacgacggc gggatcgtca gctcgagcag ccccacccgc ccgccctcca
      601 ggcggagcag gcgcaccagg tcgcccacgg tcaccgcctc ctccaccagg gcggtcagga
      661 ggtgggggac cgacacggcg acgtccacgc cccattgctc gttgaacatc cactcgttgc
      721 gcggatggtt cacgcgggcc aggacccggg gcacggcgaa ctcctgcttg gacagcaggg
      781 agatcaccag gttgtcctcg tcgtcgccgg tggcggcgac caccacgtcc gccgtcttca
      841 ggtcggcctt ctccagcacc cagggctcgc acgcgtcgcc gagcaggtac tcgaggtccg
      901 ggtcccactc ccgggcctcc ctcaagatct cggggacctg ctcgatcacc gtgacctggt
      961 gtttgcgggc ggccaggtcc gaacccaggt gacggcccac ggccccggcc cccgcgatca
     1021 cgacccgcat gtcactgctc cgccacgggg gtcaggagct cgtccagctc ggcgagcgcg
     1081 tccttgtgca cgatcagggc ggcgaagtcg ccctcctgga aggtgctgtc ggccaccggg
     1141 atgaacccgt ggccgccccg gtcgaccccc accacgcagg tcttgccgtc caggttcagc
     1201 tcccggatcg gtgtccccac caggtgttcg gggacctgca gccgcatgcg gaagaggtcg
     1261 cccccggcga ggctctcctt gatctcctcc cgggggtggg ccagcatcag catcacctgc
     1321 ttcacgcccc aggtggtggt ggccaccgtc gggatgttca gccgttcgta gatctcggca
     1381 cggcgcggat cgtagatccg ggcgatcacc ttgggaacgt ggtagtgctc gcgcgccacc
     1441 cgggcggaca cgatgttgga gttgtctccg ttcgtcaccg cgatgaaggc gtcggcgtcc
     1501 ttgatgcccg cctcctccag gatgtcccgg tcgaagccca gacccacgat ggtccgggcc
     1561 ttgaagtccg gaggcagccg gtcgaaggcg gacgggcgct tgtcgatgat cgccacctcg
     1621 tggcccgccc tgccgagctg gatcgtgagc tcggagccga ccctcccaca cccgacgatg
     1681 atcgcgtgca tcggcccgtt aggttagccg acgcggggat ccgttgaacg gccctcggtc
     1741 ccttccaccg cccagggggc tccggcgtca cggcgtccgg ggctggactg ccggcagggg
     1801 cggctcctac catcggtggc ggcatggccc ccaacggcac cgcctctccg gtcgacgtgc
     1861 tgaagcggct cctcgtcggt cgagcgctgg cctcgcacaa gcaggaacac cagctgctgc
     1921 cgaagatcct ggcgctcccg gtgttctcct cggatccgct ctcctccgtc gcctacgcga
     1981 ccgaggagat gatgctggtc ctggtggccg ccggcgccgg cgcgctctcg ctgaagctgc
     2041 cgatcgcggt ggggatcgct ctgctgctgg cgatcgtcgt cacgtcgtac cggcaaacgg
     2101 tccgcgccta cccccagggc ggcggctcgt acatcgtggc tcgtgagaac ctcgggacgg
     2161 tgccgggtct ggtggccgct gccgcgatcc tcaccgacta cgtcctcacc gtggccgtca
     2221 gcgtgacggc cggaacgatc gccatcacct ccgcgttccc ctcgctggtc ggggaccggg
     2281 tgccgatcgc gatcgcgttg gtgggtctgg tgacgatggt gaacctccgg ggggttcggg
     2341 agtcgggcat agtcttcgcg gtccccacct acggcttcgt ggccctggtg atggtgaccc
     2401 tggccgccgg gttcgtggag tgcgcgacgg gttgtccgcg ggcggccacg gcggatctgc
     2461 cgctgcaggc cgaggccggc ttggggctgt tcctggggct gaaggcgttc tcctcgggtg
     2521 ccacggcgct caccggcgtg gaggcgatcg ccgacggtgt cccggccttc cgtcggcccc
     2581 agtcacacaa cgccgccacc acgctggcca tgatgggcgt gatctcgatc acgatgttcc
     2641 tgggcatcac ggcgctcgcg gagttcctgc gggtgcgggt cacggcggag ctggccgcgt
     2701 cgcggtcggt gctgtcgcag atcggggaga cggtgttcgg gcgcaacgcg ctgttcttcc
     2761 tgctgcaggt gttcacggcc gggatcctgg tgttggcggc caacacctcc ttccaggact
     2821 tcccccggtt gtcctcgatc ctggccaggg accggttcat gccttcgcag ttccggaacc
     2881 ggggtgaccg gctggtcttc tccaacgggg tcctggtgct ggcgctgttg gcatcggcgc
     2941 tgatctgggt cttccatgcg cagctcaccc atctgatcca gctgtacgtg gtgggggttt
     3001 tcaccgcgtt cacgctctcg caggcgggga tggtgcgccg gtggatgcgt cggaggggcc
     3061 cgcagtggcg gcggagcgcg atcatcaacg gcatcggggc gagcgtcacc ggggtggtcc
     3121 tggtgatcgt cacgatcacg aagttccggg gcggcgcctg gatcgtgatc acggcgatcc
     3181 cggtcatcgt ggcgttcttc ctcgccgtgc accaccacta cgagcgggtg gggacgatgc
     3241 tgcgggcccg cgatctggcg gtcgatgccg agcgtcggaa cgcggtggtg ttcgtggtgg
     3301 ccgatctggg gccggccacg atcgacgccg tggcgtacct gcgggccctg cgccccgagc
     3361 gggtggagcc ggtgttcgtg gggcccgcgg acgcatacga gcgcgttgcc accgcctggg
     3421 atcatcgggc accgcggttc gggcgcctgc acccgttgcg gcaggcccag ctccacccgg
     3481 tgcgggcgct ccgggaccac gtccgggcca tggcccggac ccaggaggag ttcgtcacgg
     3541 tgctcatccc cgagctcctc cacagccaca cgtggcgaca gttcctccgc cggcggcaga
     3601 ccctgctgct gaaggcggcc ttcctgttcg aacccgacat cgtggtgatg aacgtcccgc
     3661 tcttgcccga ggagctcctg gatgccgagg cccatcgcca ccacacggcg gagctcgagc
     3721 ggaccgtcgc gctggtcccg gtctccgcgg tgcacgacgc cacggtcgag gcggtgtcgt
     3781 acgcgatctc cttgaacccg tggcgggcgg aggccctgtt cctggccacg gatctgaacg
     3841 aggtgccggc catcatcgag cagtggggca acaagcagat gccgatcccc ctgtccgcga
     3901 tcgaggcgcc cttccgtgac atcggcgccc ccctgctcga ggaggtgcgc aagcacaccg
     3961 accgcggtga cacggtggtg accgtggtga tccccgagtt cgtggtgcat cggtggtggg
     4021 agcacctgct ccacaaccag acggccttct acatcaagcg cctgttgctc ttcgagcccc
     4081 gcgtggtggt ggccagcgtg cccttccacc tgggcgccga gagccgggcc gccacggcgg
     4141 ccgcgtcgta gcatcctccc gccatgtcgg agttgagctc ccgccggatc cagggtcgcg
     4201 tgggcggcta cctcaagcgc gtgctcgtgg gccgggccgt ggccagccag aagctcgagc
     4261 acaccctgct gccgaagatc ctcgccctgc cggtgttctc gtccgacgcc ctgtcctcgg
     4321 tggcgtacgc gacggaggag atcctgaccg tcctcctggg gatcggggtg ggtgccgtgg
     4381 cctatccgaa ggtcttgccg atctcggtgg ccatcgcgtt gctgatggcg atcgtggtga
     4441 cctcctaccg gcagacggtc cgcgcgtacc cgaacggggg cggggcctac atcgtgagca
     4501 aggacaacct cgggacggtc cccggactgg tggcggccgc ggccctgctg gtcgactacg
     4561 tgctcacggt atcggtctcg atcgtggccg gcgtgttcgc gctggtctcg gcggcccccg
     4621 ccctgaacca gcacaaggtg atcctctccc tggtgttcat cgcgttcatc accctggcga
     4681 acctccgcgg cgtccgtgag tcggggacgc tgttcgcggt tcccacctac gccttcatcc
     4741 tgtcgatcct gtcgatgatc gtggtgggct tgatcggctg cgtggggggc tgcccggccg
     4801 cccagccggt gacgccgctg ccggagctgg cgggggcgac cgcggcgagc atccccctgt
     4861 tccagatcct ccgggccttc gcctccgggt ccaccgcgct caccggtgtc gaggccatct
     4921 cgaacggcgt gcccgcgttc cgccggccac aggcgcgcaa cgccggggag accctggcca
     4981 tcatggccac gatcgcgatc acgatgttcc tgggtatctc cttcctggcg agccacatgc
     5041 acgtgaccgt gagcgcggat cgctccgtgg tggcgcagat cgcccacacc gccttcgggg
     5101 gcggcttcgc cttctacatg gtccagacct tcaccacggc gatcctcgtg ctggccgcca
     5161 acacctcgtt ccaggacttc cccaggctct cggcgatcct ggccagggac cggttcatgc
     5221 cccgccagtt ccagaaccgc ggcgaccggc tggtcttctc gaacggcgtg atcgtcctgg
     5281 ccggcctggc cgggctgctc gtctacgcct tcgatgccaa cctgtcccgc ctgatccagc
     5341 tctacgtggt cggcgtgttc acgtcgttca ccctgtcgca gaccgggatg gtgcggcact
     5401 ggctgaagga gcgtcacagg ggacccgagg cccagcgcgg ttggcagcgg gcgatcgtga
     5461 tcaacgcgat cggggccgtg gccaccttca tcgtcctgat cgtggtcctg atgacgaagt
     5521 tcgagcacgg cgcctggatc gtggtcgtgg cgatccccgt gctggtggtg ctgttcatga
     5581 ccgtgcaccg gcactacgta tcggtgatga ctcagctccg gcggggcacg gtccgggccg
     5641 gggagatcgg ggtcaaccac ctcgtcttgc tggtgcgtga gctcgatgcg gcgacggccg
     5701 aggcggttgg ggtggtgcgg tcgatacggc ccaccgagct ccacgtcgtc caccccacgt
     5761 ccaacggcat cgcgccggag ctgcagcagc ggtggcgcga gttctcgatg ggcgctccgg
     5821 atctggaggt cctgccggtg tcgaacggga acctgacgca agcggtccgg gcctacatcg
     5881 gtcggttgga tcgggcctcg tcggacttcg tgaacgtcgt gatccccgag gtgatcggcg
     5941 agggccttgc cggctacctg ttccgccgct ggtccctggt gcggctgaag gccgggctgc
     6001 tgcgggagcc caacgtggtg gtgaccgacg tgccggtcca cgtggacgag ggcaacctgg
     6061 ggagggtgga cggccgcccc ctgatcccct cgcgcacggt ggccctggcc ttcgtgtccg
     6121 cggtgaacga cgccagcatc cgggccgtga actacgccca gtcccttgag gccgcggaga
     6181 cgcgggccat ctacttcgac ctcgatccgg acgttgcgca ccggatgcag gaggagtggg
     6241 gggcggccgg cctgcgaatc cccctcgaca tcgtggaggc cccgttccgg gatctgaccg
     6301 gccccatgtt ggacgaggtc cgtcggcaca ccgagcggga ggacaccgtg gtgctggtgg
     6361 tcgtccccga gttcgtggtg cgacgatgga ggcagatggt gctgcacaac cagaacgcgc
     6421 tgttcgtgaa gcgtctgttc ctgttcgagc cgcgggtggt gctctccagc gtgcccttcc
     6481 cactggagac ccgctgatgg accgggacca ggcgctcgcg ctctgccgcg agaaggccga
     6541 gaaggagacc acggtccggc acctgatctc ggtggagggg gtgatgcggg cgctgggtcg
     6601 tcggttcggg gaggaccagg acctctgggg gctgaccggt ctgttccacg acctggacca
     6661 ggacgagacg cacgacgatc ccgagcgcca cgcgttcctc gcggcgggat ggctgcggga
     6721 ggccggggtg gacgagcgga tcgtgaacgg tgtcctggcc cacgcccgcg aggagcaccg
     6781 cacggacctg atgtccaaag cggtggtcca cgccgatgcc gtggcggggc tcctggtggc
     6841 cgcggccctc gtgcgccccg agcgggccgt ggggatgaag gtctccagcg tgaagaagaa
     6901 gctcaaggag agggccttcg ctccgggcgt gaaccgcgcc gaggtgaccg gggtggacgg
     6961 ctcgatcggg ctgacgctgg acgagttcct cgcggtctcg atcgagggcc tccagctcgt
     7021 tgcccctgag atcggcctgg tcccctagcc cgccggccag gtcagagcac gcccgtccgc
     7081 tcgtgccgcc gctcgctcat caccgcgcac agctcggctc cgtacacgaa cagctgcgcg
     7141 gcgatgttga agatcgccag gacgccgatc acggcggcga acgttccgta gatggccgag
     7201 gcccggctga tcacccgggc cgcgtaccag gcgccggcga gcttcagccc cgtccatccc
     7261 gcggccatga gcagcgcccc cggcacgagg tacgggtagg agggacccga accgggagcg
     7321 agcacccggt aggcgagcag gaagaagccc aggtcgaccg ccgcagcccc ggcgatgccg
     7381 agggcccgca cggcgagacc gacccccgcc tcgctgccca gcccagccag gaacccggtc
     7441 acggacaccg agcccagggc cacgacgccg agcgcggcca gggcaccgac cgtgcggaac
     7501 cgcttcagca ccgggttgct cgccctgggg aggcggaaga tccgtgccag ggtggttcgg
     7561 gcaccgccca ccatccccat ccccgcccac agcaggccgg ccagcccgat cgcgccggcc
     7621 gtggctcggc cctcgaccac ggcccgcatg ttgctcgcga tgatggaccc cagcccgggc
     7681 acggacccgg ccagacggtt gacccactcc cgctgggccg tggggtcgtc cttcagcagg
     7741 tagccgatca ccgagagggc cagcagcagc agcgggaaca gggacaggaa ggcgtaatag
     7801 gtgagggccg ccgccatctg ccccccgttg ttctcgccgc tgcggtggcc cacggcacgc
     7861 accaggtcca ccgcgcggat gcgttggacc tgctcccaga gcgctcgccc tcggcggatc
     7921 ggtccccatc cactcagatc cacgacccac agcattccac cagggctcgg gggtcggccg
     7981 gagcggcgga cgtggaactc cgaccgggtg gatctcggac cggaccggat cagcggccga
     8041 ccggctcgcg ctcgggctct tcccccgggt cgtcttcccg ggacggcaac ggtcgcacgc
     8101 gcagctcccg gacccttcgc cagacccacc agccgatgcc gaccgccagt accgccccga
     8161 acgcccagga gaaggggcgg atcacctgct cgacccggtc ccagcgctcg cccagctggt
     8221 agccgaggat cgtgaggccg aaggtccagg gcaggcaccc gagcaacgtg tagacgctga
     8281 acctccacac gttcatcctg gcgacccccg ccggcaggct gatgaaggtc cgcaccacgg
     8341 gga
//
LOCUS       DASQQQ010000139         3118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_311110, whole genome shotgun sequence.
ACCESSION   DASQQQ010000139 DASQQQ010000000
VERSION     DASQQQ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311110"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VE646_05760"
     CDS             complement(<1..370)
                     /locus_tag="VE646_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZD79526.1"
                     /translation="MTASAPDPPPYGTGTLSDLSRSLFAAVGVPGLDDALGIGSLAGA
                     CLLLVDGLGWRALQAARDRAPFLSSLMEGAEARALSCGFPATTATSLASVGTGVPPGE
                     HGVVGYTFAMPDLDRPMNALR"
     gene            complement(367..3000)
                     /locus_tag="VE646_05765"
     CDS             complement(367..3000)
                     /locus_tag="VE646_05765"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79527.1"
                     /translation="MAAPETLGRTLAEAADPELARVALSRVGEDRLARAALAREDVLP
                     AAVRLLGFSTSAADFLVAHPEETEALADIGPRTRGQLEEELAGDVARLRPVDGLRRFR
                     RRAMLRVAAADLAGRPLEEVVGQISAVAEVCLGQGCRLVAPAELALIGLGKLGGAELN
                     YASDVDLIFVHRGDRERAERSVAELIRLLSEPTAEGIALRIDPTLRPGGRGGALSRSI
                     EAMHDYYERGSATWERQALIKARPVAGDLQLGEAFIRMVEPFVYPEELAPQAIEEVRR
                     VKVRLEDYIRARGKAGVEVKRGWGGIRDVEFAVQLLQIVHGRRDARLREPNTLTALAT
                     LADRGYVAEPDARALTEAYRFLRVLEHRLQIVRDLQTHELPSDRPSRTRLARSLGMDG
                     PEALQAEYERQTALVRSLHQRLFYRPLLEAFAGPPTPRPGVDREATEELLAGLGFGRP
                     AEAYAVLDRLVDPATRLGKVLGHVFAVMAPALALAADPDAALVRLERVAAAVGPEPEL
                     ADVLATDPGVARRIAHVVSASSFATDLLVADPSRLRALEQEAEPADVQAALVRVVGRY
                     AARELDPRDTGLTLTEVADRVVRDALGRAAPELPFAVIGLGKLGAQELNFASDLDLVF
                     VYEGDGSEDFRRAGDLGERVLAAIRQAGWEPDADLRPEGRAGPLARSIAAYLEYWERH
                     AQTWEFQSLLRARFVAGDGGLGHRFCSFARDFAYPEYLPVERVGEIRRMRDRVERERV
                     RPAEAGTFHFKLGRGSLADVQFAVELALMSHGGRHPEVRSTRTLDAVERLAQARLIED
                     SVALGLGEAFVFLSNVKNALEVDRRVHAEALPPSPEEQTALARRLGYEEYPRQSFLGD
                     YLRIARRARRAMERVFAEVTA"
BASE COUNT          455 a         1215 c         1078 g          370 t
ORIGIN      
        1 accggagcgc gttcatcggc ctgtccaggt cgggcatcgc gaacgtgtaa cccaccacgc
       61 cgtgctcgcc tggcgggacc ccggtcccca ccgaggccag gctggtggcc gtggtggccg
      121 ggaacccgca ggacagggct cgggcctctg cgccttccat cagcgaggac aggaacggcg
      181 cccgatcccg ggcggcctgc agcgcccgcc agccgagccc gtccaccagc agcaggcacg
      241 cgccggcgag cgacccgatc ccgagcgcgt cgtccaggcc cggaaccccc acggccgcga
      301 acagcgagcg agacaggtcg ctcagcgtcc ccgtgccgta gggaggcgga tcgggagccg
      361 acgcggtcac gcggtcacct cggcgaagac ccgctccatg gcccgacgcg cccggcgggc
      421 gatccgcaga tagtccccca ggaagctctg gcgcgggtac tcctcgtacc ccaggcgccg
      481 ggccagagcg gtctgttcct cgggggatgg agggagcgcc tccgcgtgca cccgccggtc
      541 gacctcgagc gcgttcttca cgttcgacag gaagacgaac gcctccccca acccgagggc
      601 cacgctgtcc tcgatcagcc gggcctgggc cagccgctcc accgcatcca gggtgcgcgt
      661 ggatcggacc tcgggatgac ggcccccgtg gctcatcagc gccagctcca cggcgaactg
      721 cacgtccgcc agggagcccc ggcccagctt gaagtggaag gtgccggcct ccgcggggcg
      781 aacccgctcg cgctccaccc ggtcacgcat ccgacggatc tcgcccaccc tctccaccgg
      841 caggtactcg gggtaggcga agtcacgcgc gaacgagcag aatcggtggc cgaggccccc
      901 gtcccccgcc acgaacctcg ctcggagcag cgactggaac tcccaggtct gcgcgtgccg
      961 ctcccagtac tcgaggtagg cggcgatcga gcgcgccagc ggcccggctc gaccctctgg
     1021 ccggaggtcg gcgtccggct cccacccagc ctggcggatc gcggccagga cccgctcccc
     1081 caggtcgcct gcgcgccgga agtcctcgga gccgtccccc tcgtacacga acaccaggtc
     1141 caggtccgag gcgaagttca gttcctgagc tcccagcttg cccaggccga tcaccgcgaa
     1201 gggcagctcc ggtgcggccc gccccagggc atcgcgcacc acccggtcgg ccacctccgt
     1261 gagggtcagg cccgtgtccc gggggtcaag ctcccgagcc gcgtaccggc ccaccacccg
     1321 gaccagggcg gcctgtacgt ccgccggttc cgcctcctgc tccagcgccc ggagtcgcga
     1381 ggggtcggcc accagcaagt cggtggcgaa cgagctggcc gacacgacgt gcgcgatccg
     1441 tcgagccacc ccgggatcgg tggccagaac gtcggccagc tcgggctcgg gacccaccgc
     1501 ggccgccacc cgctccaggc ggaccagggc ggcgtccggg tcggcggcca gcgccagggc
     1561 cggagccatc accgcgaaga cgtgcccgag gaccttgccc aggcgcgtgg cgggatcgac
     1621 cagccggtcg agcacggcgt aggcctcggc cggacggccg aatcccaggc cggccaggag
     1681 ctcctcggtg gcctcccggt ccacgcccgg tctgggggtg gggggcccgg cgaaggcctc
     1741 tagcagggga cggtagaaca ggcgctggtg gaggctccgg accagcgcgg tctggcgctc
     1801 gtactcggcc tggagggcct cgggcccgtc catgcccagg gagcgcgcca gccgggtccg
     1861 cgaggggcga tcggacggca actcgtgcgt ctgcaggtcc cggacgatct ggagccggtg
     1921 ctccagcacg cgcaggaacc gataggcctc ggtgagcgca cgagcgtccg gctcggccac
     1981 gtaaccccgg tcggcgagcg tggcgagcgc ggtcagggtg ttcggctcgc gcagccgggc
     2041 atcccgccgg ccgtggacga tctgcagcag ctggacggcg aactccacgt cccggatccc
     2101 tccccacccc cgcttcacct ccacgccggc cttgcctcga gcacggatgt agtcctccag
     2161 gcggaccttg acgcgacgga cctcctcgat cgcctgaggg gcgagctcct ccggatacac
     2221 gaacggttcg accatccgta tgaaggcctc ccccagctgg agatcgccgg ccaccggccg
     2281 ggccttgatc agcgcctggc gctcccaggt cgcggaccct cgctcgtagt agtcgtgcat
     2341 cgcctcgatc gaacgagaga gggctccgcc ccgaccaccc ggccgaaggg tgggatcgat
     2401 ccggagggcg atgccctcgg ccgtgggctc cgagagcagc cggatcagct ccgccacgct
     2461 tcgctcggcg cgttcccggt ccccccggtg cacgaagatc aggtcgacgt ccgaggcgta
     2521 gttcagctcg gccccgccga gcttccccag gccgatcagg gccagctcgg ccggcgccac
     2581 gagtcgacag ccctgtccca gacagacctc ggcgacggcg ctgatctgcc cgaccacctc
     2641 ctccagcggc cgaccggcga ggtcggcggc cgccacccgg agcatggccc gccggcggaa
     2701 cctgcggaga ccatcgaccg gtcgcagcct cgccacgtcg ccggccagct cctcctccag
     2761 ctggccacgg gtccgcgggc cgatgtcggc cagcgcctcg gtctcctccg ggtgtgcgac
     2821 cagaaagtcg gcggctgacg tggagaagcc gaggagccgg accgcggccg gcaggacgtc
     2881 ctcccgggcc agcgccgctc gagccaggcg atcctcgccg acccgggaca gcgcgacccg
     2941 ggcgagctcc gggtcggcgg cctcagccag cgtccgcccg agcgtctccg gtgcggccac
     3001 ggccccccgt ccgtcggtcc cgccatcggg tctggacggc cacgagcggc gggaacagca
     3061 ccatggccat gcctatgaac agagcccccg accaggccgc gctggccaga gcccccac
//
LOCUS       DASQQQ010000140         8542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_176495, whole genome shotgun sequence.
ACCESSION   DASQQQ010000140 DASQQQ010000000
VERSION     DASQQQ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8542
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176495"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VE646_05770"
     CDS             complement(<1..663)
                     /locus_tag="VE646_05770"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZD79528.1"
                     /translation="MSPVALVGVCAGLAVVAAAGVLSPLRGSIARTERAPARGTRSSG
                     NGSARARPARADRLRGSAPALVAGLALVAVTVPFLLGAFGRSNGAPSADATPMSAIEG
                     FRQQVAEHPEDVAARLELGFLLLNQGDAGGAAQQYMAVLQRDPTNAEAHTQLAWVLLG
                     VGRPRAALQQVDQALQARTGYAEALWVRGVILLRGLHRPRSAAQAFTAYLKASPYGAH
                     RQE"
     gene            complement(660..1067)
                     /locus_tag="VE646_05775"
     CDS             complement(660..1067)
                     /locus_tag="VE646_05775"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HZD79529.1"
                     /translation="MVCAVATLGTLLLGPLSSQSAPPTRAEQVRSVAATLRCPVCLNL
                     SVADSTSGVARQMRRTIAQELSAGMTPDQIRSRFVGSYGRWILIEPPRRGLDLTIWLV
                     PALIVAAGLVWTVLAVRRWSRGAAEAGGAEELA"
     gene            complement(1103..1663)
                     /locus_tag="VE646_05780"
     CDS             complement(1103..1663)
                     /locus_tag="VE646_05780"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZD79530.1"
                     /translation="MTETKTGWTRRRWMLTGGVAAAVAIFTLPLAFGLTRDPNVTRSP
                     LLGKAAPPFTLTRLNGEGRLSLQELRGHVVVLNFWASWCVECRVEHDALAAAWARFRD
                     QGVVLVGVSFQDPPSAARGFVRATGADWPQVQDPGTRTALAYGVSGVPETFLIGPDGR
                     IAYRWIGPVSYDALAERVDRLVGGTS"
     gene            complement(1660..3660)
                     /locus_tag="VE646_05785"
     CDS             complement(1660..3660)
                     /locus_tag="VE646_05785"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HZD79531.1"
                     /translation="MGVLGRFALVLALLLAAYGAVAAVLGARWNRPALVESARTTAFS
                     SLAVIGAAYGALLAAVMANDFSLRYVAENSSRATPTFFKVLSLWSADEGSLLLWNLIL
                     AAFVAAVAFRFRRDRPASFPYALATLHVVQVFYLVLVVGPASPFTQLASPPTDGRGPL
                     PLLQNHPLMAAHPPMLYLGFIGFTIPFAFAMGALISGRLSEPWVALTRRWTLAAWVFL
                     SAGLVLGALWSYGVLGWGGYWAWDPVENVALLPWLAATAFLHSSVLEERRGMFRTWNL
                     SLVVGAFALTTFGTFLTRGSVLLSVHAFAASLVGPLYLGFLVLVMLVGFGLIAARAVQ
                     LRAPARLERAVSRGSAFVAQNVLLVAITLIVLLGTIFPLAMEAITGSRVSVGGPYFDQ
                     TTVPLFLLLLFLMGVGPLLSWRRAAPGEAARRLTVPAGATAAVTVLLAVAGVHGFGAL
                     LAFGLATFVLTANGAGLIRRIGATRRTRSLGTFRAVGSTWSEDPRRFGGLVVHLGIAI
                     AAIAITASACFTQQTEVTLARGEATSFAGYRLRYQERRVLQQPQREVLVADVAVTRGG
                     APAGTVDPSLNVYPSSTDPIGTPSIRYGVRADLYASVLGFSADGSTATLRFYHTPGVS
                     WLWMGGAIVILGGVLALRPRPRRSPPAVVAPVDRPVAEGVPA"
     gene            complement(3660..4082)
                     /locus_tag="VE646_05790"
     CDS             complement(3660..4082)
                     /locus_tag="VE646_05790"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HZD79532.1"
                     /translation="MKRGRTLFAVTVLGASLGWVALGGLRNSLVYYRTPTEVVQQGKA
                     VVGERMRLGGLVMAGTVHRAGSEIRFVVTDGTTQIPVVDTANAPALFGERKGVVLEGT
                     YGADGVFHADTMLVKHDDTYRPPAMASGASVAAAAGGS"
     gene            complement(4079..4213)
                     /locus_tag="VE646_05795"
     CDS             complement(4079..4213)
                     /locus_tag="VE646_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79533.1"
                     /translation="MAEPGYVIVGYLLTAIVLGAYVLSLFRRAARASRASGHRRRDRA
                     "
     gene            complement(4206..4958)
                     /gene="ccsA"
                     /locus_tag="VE646_05800"
     CDS             complement(4206..4958)
                     /gene="ccsA"
                     /locus_tag="VE646_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HZD79534.1"
                     /translation="MRGGRLLGRATGRRAERVLGLLALMGVAVSTVLSLVVSPPDAEQ
                     GDLIRLMYIHVPAAWLAYLSFSVVFVASVAYLRTGRSRWDRLAAASAEVGVVFTALTI
                     VLGAIWAKPVWGTWWTWDPRLTTTAILLLIYVGYLAVRRLPETPSRRGRWAAVVGIAG
                     FVDVPIVHLSVRWWRSLHQPPARLLGVPNLAPAMGVALMAGIVAFTLAYAYLLVVRLR
                     VGRAEDRQAAEAIARLTAAPSDPRLEEVTSRG"
     gene            complement(4955..5632)
                     /locus_tag="VE646_05805"
     CDS             complement(4955..5632)
                     /locus_tag="VE646_05805"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HZD79535.1"
                     /translation="MSLLRQALLVGSKDLRIELRGRHATGAAAPFVATVLLSFGLALG
                     PGRSLLRQTAPGLLWLAVLFASTMAFRSSYRAETEDGAIDSLVLSPVDDAAVFLGKAA
                     AVSVELLALEALVLALAAGLFDLSLVGQPLVLLAAVGLGTVGLSLVGSLFGIVAESSA
                     TGEGLLPLLVLPLATPVLLAGVRATAMATSGHGAEAGSWLGLLLAFDLCFAAVGVLAF
                     GALMEER"
     gene            complement(5629..6426)
                     /gene="ccmA"
                     /locus_tag="VE646_05810"
     CDS             complement(5629..6426)
                     /gene="ccmA"
                     /locus_tag="VE646_05810"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HZD79536.1"
                     /translation="MTVDRGPTAGGGGGRIRATGERNDQVTGLQSIGVGAPTDEGAVR
                     AALDPPTGAVPAVRLTGVTRLFDRQAALRNVHLHVAPGEVVMLRGPNGAGKSTLLRII
                     STAIAPTYGTGSVLGHDLRSGRAGIRAAVELLGHRARLYEDLSARENLRFLCSLYGID
                     EDGIGVALQRVRLSSAADERVRSYSQGMRQRVAIARLLLRDPGLLLLDEPYGGLDEEG
                     KGLVDELIADARTRGRTVLVATHDRSRDPSPVREVWMEDGRIVAGGA"
     gene            6688..7653
                     /locus_tag="VE646_05815"
     CDS             6688..7653
                     /locus_tag="VE646_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HZD79537.1"
                     /translation="MADVMPLRGEGRTVGSIAGLWGECLAEFLGTFVLIAFGDGVVAV
                     AVAGLPGSGRTASPTTFFQGSGDWLLITWGWALAVAFGVWVAGGISGAHINPAVTVAF
                     AVRRKFPWNKVGPYIAAQVVGAFVGAALVFLVYSAAINAFNAAAGVTKSSGQALVTFS
                     IFATFPAGYFHGGWVGPFIDQIVGTAFLVMFVVAIIDARNQPVQSNLAPLIVGLVVAA
                     VGMSYGPNAGYAINPARDFGPRLFAWVAGWGSVALPGTLHIKGLDFSNYVWIPIVGPL
                     LGGPIGVIVYDWFIGDVLHARLKPPPPGRIPEAMSGPTTSEPPPG"
     gene            7729..>8542
                     /gene="mmuM"
                     /locus_tag="VE646_05820"
     CDS             7729..>8542
                     /gene="mmuM"
                     /locus_tag="VE646_05820"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF007020.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HZD79538.1"
                     /translation="MEDPIRALLRARGTVVLDGGLATELERRGADLRDPLWSARVLVE
                     DPSMILEVHRSYFEAGADVAISASYQASFQGLEARGLDTDAAAALMRSSVELADRARL
                     EAPGDGSRRLVAASVGPYGAVLANGAEYTGDYDRTADWLREWHLLRLEVLATSGADLL
                     AVETIPSIVEAEALTEALTRFPEVPAWVSFSCRDGARICDGTPFERAARVATTAPNVV
                     AVGVNCTAPSHVASLVELATAAVDRPVVTYPNRGAFWDPVRRAWTDPPRQDAG"
BASE COUNT         1357 a         3189 c         2958 g         1038 t
ORIGIN      
        1 ctcctgccgg tgcgccccgt acggtgatgc cttcaggtag gcggtgaatg cctgcgccgc
       61 cgatctcggc cggtgcaggc cgcgcagcag gatcacgccg cggacccaca gggcttccgc
      121 gtagccggtc cgagcctgga gcgcctgatc cacctgctgc agcgccgcac gcggtcgtcc
      181 gaccccgagc aggacccagg ccagctgcgt gtgggcctcg gcgttcgtgg gatcacgctg
      241 caggacggcc atgtactgct gcgcggcccc gcccgcatcg ccctggttca gcaggaggaa
      301 cccgagctcc agtcgggccg ccacgtcttc ggggtgctcg gccacctgct ggcggaagcc
      361 ctcgatggcg gacatgggcg tggcgtccgc gctcggggcg ccgttggaac gaccgaacgc
      421 accgaggagg aaggggaccg tcaccgcgac gagcgccagg ccggccacca gcgccggggc
      481 gctgccccgc aggcgatcgg cccgtgccgg gcgtgcccgc gccgatccgt tccccgacga
      541 tcgtgtccct cgagcaggcg cccgctccgt ccgtgcgatg ctgccacgca acgggctcag
      601 gaccccggcc gcggccacga cggcgagacc cgcgcacacc ccgaccagcg cgaccgggct
      661 catgccagct cctccgcgcc gccggcctcg gccgcccccc ggctccagcg gcgcaccgcg
      721 agcacggtcc acaccagtcc ggccgccacg atcagcgccg gtaccagcca gatcgtcaga
      781 tccagacccc gacggggcgg ctcgatcagg atccaccggc cgtaggaccc cacgaagcgg
      841 gagcggatct ggtccggggt catcccggcc gacagctcct gggcgatcgt tcgtcgcatc
      901 tgcctcgcca cgcccgacgt ggagtcggcc acactgaggt tcaggcagac ggggcagcgc
      961 agggtggcgg ccaccgagcg cacctgctcg gcccgggtgg gtggggcgct ctggctgctc
     1021 agaggcccca gcagcagggt gccgagcgtc gcgaccgcac acaccaccag gaagatggtt
     1081 cgaggacgac ctaccgccat cctcacgagg tccccccgac caggcgatca acgcgctcgg
     1141 ccagcgcgtc gtacgagacc ggcccgatcc accggtaggc gatgcggccg tcaggaccga
     1201 tcaggaacgt ctcggggacc ccggagacgc cataggccag cgcggtccgc gtccccgggt
     1261 cctgcacctg gggccagtcc gcgcccgtgg ccctcacgaa cccgcgggcc gcggatggcg
     1321 ggtcctggaa cgagacgccc accagcacca ccccctggtc gcggaagcgg gcccaggcgg
     1381 ccgccagcgc gtcgtgctcc acgcggcact ccacgcacca ggaggcccag aagttcagca
     1441 ccacgacgtg accgcgcagc tcctgcagcg acagccgtcc ctcgccgttc aggcgggtca
     1501 acgtgaacgg aggagccgcc ttgcccagca agggggacct cgtgacgttg gggtcgcgag
     1561 tcaggccgaa ggcgagcggg agcgtgaaga tggccaccgc cgcggccacc ccaccggtca
     1621 gcatccaccg gcgacgggtc cagcccgtct tcgtctccgt cacgccggga ccccttccgc
     1681 gacgggtcgg tccaccggcg cgaccacggc ggggggcgat cggcggggcc ggggacgcag
     1741 ggccaggact ccgcccagga tcacgatggc cccgcccatc cacagccagg agacaccggg
     1801 cgtgtggtag aagcggaggg tcgccgtgga cccgtcggcg ctgaagccga gcacggacgc
     1861 gtacaggtcg gctcggaccc cgtaccggat cgagggcgtc ccgatcggat cggtggaact
     1921 cggatagacg ttcagggagg ggtccacggt gccggccgga gcgccgcccc gggtcaccgc
     1981 cacgtcggcc accagcacct ctcgctgggg ctgctggagc acccgccgct cctggtaccg
     2041 gagccggtag ccggcgaagg aggtggcctc gccgcgggcc agggtgacct cggtctgctg
     2101 cgtgaagcag gcggaggcgg tgatcgcgat cgccgcgatt gcgatgccca ggtgcaccac
     2161 caggccgccg aaccgtcggg gatcttcgga ccaggtcgag cccaccgccc ggaacgtccc
     2221 caacgagcgc gtccgccggg tcgccccgat ccgacggatc agcccggcgc cgttcgcggt
     2281 cagcacgaac gtagccagcc cgaaggccag cagcgccccg aacccgtgga cgcccgccac
     2341 ggccagcagc accgtgaccg ccgcggtagc gccggccgga acggtcagtc gcctggcggc
     2401 ctcgccggga gcagcccgcc gccacgacag cagcggaccg acccccatca ggaacaggag
     2461 cagcaggaac agcggcacgg tggtctggtc gaagtaggga ccgcccacgg acacccggct
     2521 cccggtgatc gcttccatgg ccagggggaa gatcgttccc agcagcacga tgagggtgat
     2581 ggccaccagc aggacgttct gcgccacgaa cgccgacccg cgggagaccg ccctctccaa
     2641 cctggccggc gcccggagct ggaccgcccg ggccgcgatc agaccgaacc ccaccagcat
     2701 caccagcacc aagaacccca ggtacagcgg accgaccagg gaggccgcga aggcgtgaac
     2761 cgacaacagc acgctgccgc gggtgaggaa ggtcccgaag gtggtcagcg cgaacgcgcc
     2821 caccaccagc gagaggttcc aggtccggaa catgccccgt cgctcctcca gcacggagga
     2881 gtgcaggaag gcggtggccg ccagccaggg gagcagcgca acgttctcca ccgggtccca
     2941 ggcccagtag ccaccccacc ccaggacccc gtacgaccag agcgcgccga gcaccagccc
     3001 cgcggagagg aagacccagg ccgccagcgt ccaccggcgg gtgagcgcga cccacggctc
     3061 ggagagccga ccggagatca gggcgcccat ggcgaaggcg aaggggatcg tgaacccgat
     3121 gaagcccagg tacagcatgg gggggtgcgc cgccatcagg gggtggttct ggagcagcgg
     3181 cagcggacca cggccgtccg tgggcgggga ggcgagctgc gtgaacggcg atgccggccc
     3241 caccacgagc accaggtaga acacctggac cacgtgcagc gtggccagcg cgtaggggaa
     3301 cgaggccggc cggtcgcggc ggaaccggaa ggccacggcg gccacgaatg cggccaggat
     3361 caggttccag agaaggagcg atccctcatc ggcggaccac agggatagga ccttgaagaa
     3421 cgtgggcgtg gcccggctgg agttctcggc cacgtaccgc aacgagaagt cgttggccat
     3481 gaccgccgcc agcaacgccc cgtaggcggc accgatcacg gcgagcgacg agaacgcggt
     3541 ggtgcgggcg ctctccacca gggccggccg gttccagcgg gccccgagca ccgccgccac
     3601 cgctccgtag gcggccagca gcagcgcgag caccaacgcg aaccgcccca ggacgcccat
     3661 caggaccccc cggcggccgc ggcgaccgac gcgccggaag ccatcgcggg cggccggtac
     3721 gtgtcgtcgt gcttcaccag catcgtgtcg gcgtggaaga cgccgtcggc tccgtacgtg
     3781 ccctccaaga ccacgccttt gcgctcaccg aacagcgccg gagcgttcgc cgtgtccacc
     3841 accgggatct gcgtggtgcc gtcggtcacg acgaatcgga tctcggagcc ggcgcggtgc
     3901 accgtccccg ccatcaccag cccccccagg cgcatgcgct cgcctacgac cgccttcccc
     3961 tgctggacga cctcggtggg ggtccggtag tagacgaggc tgttccgcaa cccccccagc
     4021 gccacccatc ccaggctcgc gccgagcacc gtcaccgcga agagcgtccg gccccgcttc
     4081 acgcgcgatc ccgccgccgg tgtccggaag cccgcgaggc gcgcgcggcc cgccggaaga
     4141 gcgagagcac gtacgcgccg aggacgatgg cggtgaggag gtagccgacg atcacgtagc
     4201 cgggctcagc cacggctggt cacctcctcc agccggggat cggacggcgc ggcggtgagg
     4261 cgggcgatgg cctccgcggc ctgccggtcc tccgcccgcc ccacacgcag gcgcacgacg
     4321 agcaggtacg cgtaggccag cgtgaacgcc acgatcccgg ccatcagcgc cacgcccatg
     4381 gccggggcca ggttcggcac gcccaacaac cgggccgggg gctggtgcag cgaccgccac
     4441 caccgcaccg agagatggac gatcggcacg tcgacgaagc cggcgatccc caccaccgcc
     4501 gcccaccggc cacggcgcga cggcgtctcg ggcagccggc ggaccgccag gtagcccacg
     4561 tagatcagca gcaggatcgc cgtggtggtg agcctcggat cccaggtcca ccacgtgccc
     4621 cacaccggct tggcccagat cgctcccagg acgatcgtga gcgccgtgaa caccaccccc
     4681 acctcggccg aggccgccgc caaccggtcc cagcggctcc ggccggtccg taggtacgcc
     4741 acgctggcca cgaacaccac cgagaaggac aggtacgcga gccaagcggc cgggacgtgg
     4801 atgtacatca gccggatcag atctccttgc tcggcatccg gcggcgagac cacgagggac
     4861 aggaccgtgc tcacggccac ccccatcaac gccaggaggc cgaggacacg ctcggcccgc
     4921 cgcccggtcg ccctccccag cagtcgcccg cccctcatcg ctcctccatc aacgcgccga
     4981 acgccaacac ccccacggcc gcgaagcaca ggtcgaaggc caacaacagc cccagccagg
     5041 agccggcctc ggccccgtgg ccggaggtcg ccatcgcggt cgcacgcacc ccggcgagca
     5101 gcaccggtgt agccagcggc agcaccagca acggcaacag cccctccccc gtcgcggagg
     5161 actcggccac gatcccgaac aggctgccca ccagcgacaa ccccaccgtg ccgagcccga
     5221 ccgccgccaa gagcaccagg ggctgcccca ccagcgacag atcgaacagc cccgccgcga
     5281 gagccagcac cagggcttcc agcgccagga gctccacgga gaccgcagcc gccttgccca
     5341 ggaagacggc ggcatcatcc acgggggaca ggaccaggct gtcgatcgcg ccgtcctcgg
     5401 tctcggctcg gtagctgctg cggaacgcca tggtcgacgc gaagaggacc gcgagccaca
     5461 gcaggcccgg agcggtctgc cgcagcaggg acctgcccgg accgagcgcc aggccgaacg
     5521 acagcagcac ggtggccacg aagggggccg cggccccggt ggcgtgacga ccccggagct
     5581 cgatcctcag gtccttgctc cccaccagca gcgcctgacg gaggaggctc atgcgccgcc
     5641 cgccacgatc ctgccgtcct ccatccagac ctcccggacg ggggagggat cacgcgagcg
     5701 gtcgtgcgtg gccacgagca cggtccggcc tcgggttcga gcgtcggcga tcagctcgtc
     5761 caccagcccc ttgccctcct cgtccagccc gccgtagggc tcgtccagca gcaggagccc
     5821 ggggtcgcgc agcagcaacc gggcgatggc tacccgctgg cgcatgccct gcgagtagga
     5881 gcggacgcgt tcgtcggccg ccgacgacag cctcacccgc tgcagtgcca ccccgatgcc
     5941 gtcttcgtcg atcccgtaca gggagcagag gaagcgcagg ttctcgcgcg cgctgaggtc
     6001 ctcgtacaac cgggctcggt gaccgaggag ctcgacggcc gcacggatcc ctgctcggcc
     6061 cgagcgcaga tcgtgcccga gcaccgaccc ggtcccatag gtcggcgcga tcgccgtgga
     6121 gatgatcctc aggagcgtgc tcttgcccgc gccgttcggg ccgcggagca tcacgacctc
     6181 acccggagcg acgtgcaggt ggacattccg cagggccgcc tgacgatcga agagcctggt
     6241 gacgccggta agccgtaccg ccggcaccgc accggtcggt ggatccaagg ccgcgcggac
     6301 ggcaccctcg tcggtcggag cccccacgcc gatcgactgc agacctgtca cctggtcgtt
     6361 ccgctccccc gttgccctga tgcgtccgcc gcccccgccc gcggtgggcc cgcggtcgac
     6421 cgtcaccccg caccggcagg ccatccaccg gctcgccggg tatacccccc cgcaaccgcc
     6481 cccaaacctc ccggacgctt tgtcgggcaa gggtcccggc ttgacatttc gagtcggaaa
     6541 gcgccttctt gttccttgca tggatccaag ggcggagccg cgcacgcttg ggcgcgggcg
     6601 gttgcccggt gaggaggtga tacccactgg acgatcgagc gtctgggacg gctcgatccg
     6661 atcggcaccg accttcgagg aggtgacatg gcagacgtga tgccgttgcg gggcgagggc
     6721 aggaccgtcg ggtccatcgc cgggttatgg ggcgagtgcc tcgcagagtt cctcgggacg
     6781 ttcgtcctga tcgcgttcgg tgatggcgtg gtggcggtgg cggtggcggg actgcccggc
     6841 tcggggagga ccgccagccc cacgaccttc ttccagggct ccggggactg gctgctgatc
     6901 acctggggat gggcgctcgc tgtggccttc ggcgtttggg tggccggagg gatcagtggc
     6961 gcgcacatca accccgcggt gacggtggcc ttcgcggtcc ggcgcaagtt cccctggaac
     7021 aaggtcgggc cgtacatcgc ggcccaggtg gtcggtgcct tcgtgggcgc cgccctcgtg
     7081 ttcctggtct acagtgcggc gatcaatgcg ttcaacgccg ctgccggggt gacgaagagc
     7141 tccggccagg ccctggtgac gttctcgatc ttcgccacct tcccagctgg ctacttccac
     7201 ggcggctggg taggaccctt catcgaccag atcgtcggta cggcgttcct ggtgatgttc
     7261 gtggtggcga tcatcgacgc ccgcaaccag ccggtccaga gcaacttggc ccccctgatc
     7321 gtcgggctcg tcgtcgccgc cgtgggcatg tcctacggcc ccaacgccgg atacgccatc
     7381 aacccggccc gtgacttcgg gccgaggctg ttcgcgtggg tggccggctg gggctcggtg
     7441 gccctccccg ggaccctgca tatcaagggg ctcgacttca gcaactacgt gtggatcccc
     7501 atcgtgggtc cgttactcgg ggggcccatc ggcgtgatcg tctatgactg gttcatcggc
     7561 gacgtgctgc atgcgaggct gaagccccca cccccgggtc ggattccgga ggccatgtcg
     7621 ggaccgacca cgagcgagcc gccaccgggc tgagctgcgc tctccgcatc gacgaggccg
     7681 gcgggaacct cccgccggcc tccgtcatgc gtcgactagg ctgagctcat ggaggatccg
     7741 atccgggcct tgctccgagc tcgaggaacc gtggtgctcg acgggggcct ggccaccgag
     7801 ctcgagcggc ggggagccga ccttcgcgac cccctgtggt cggcccgggt cctggtcgag
     7861 gacccgtcga tgatcctcga ggtccatcgc tcctacttcg aggctggggc cgacgtcgcg
     7921 atctcggcca gctaccaggc gagcttccag ggcctggagg cccggggact cgataccgat
     7981 gcggccgcgg ccctcatgcg ctcgagcgtc gagctggcgg accgagcccg tctggaggca
     8041 ccgggggacg gctcccgccg gctggtggcg gcttcggtcg gtccctacgg cgccgtgctg
     8101 gccaacgggg ccgagtacac aggcgactac gaccggaccg cggactggct gcgggagtgg
     8161 cacctgctcc gtctggaggt cctggccacc tcgggcgccg acctgctggc cgtggagacc
     8221 atcccctcga tcgtggaggc ggaggcgctg accgaggccc tgacccggtt ccccgaggtt
     8281 cccgcgtggg tcagcttcag ctgtcgcgac ggggcccgga tctgcgacgg gacgccgttc
     8341 gagcgcgccg cccgcgtggc caccaccgcg cccaacgtgg tcgccgtggg cgtcaactgc
     8401 acggccccca gccacgttgc ctccctggtg gagctcgcga ccgctgccgt cgaccggccg
     8461 gtggtgacct acccgaaccg cggagcgttc tgggacccgg tccgccgcgc ctggaccgat
     8521 ccaccccggc aggacgcggg tc
//
LOCUS       DASQQQ010000141         2320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_148857, whole genome shotgun sequence.
ACCESSION   DASQQQ010000141 DASQQQ010000000
VERSION     DASQQQ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148857"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /gene="sat"
                     /locus_tag="VE646_05825"
     CDS             complement(<1..319)
                     /gene="sat"
                     /locus_tag="VE646_05825"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF025664.4"
                     /note="ATP sulfurylase; ATPS; converts ATP and sulfate to
                     5'phosphosulfate and pyrophosphate; in some organisms this
                     enzyme is involved in the incorporation of inorganic
                     sulfate while in others it is involved in the production
                     of ATP in the reverse direction; the enzyme from Thermus
                     thermophilus is dimeric and binds a zinc ion that is
                     coordinated by cysteine and histidine residues that are
                     not found in all related proteins but is found in some
                     thermophilic organisms; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HZD79539.1"
                     /translation="MQRQLAVAEAEELAARRIPRLTLSAAEVADLRALATGAYSPLGG
                     FMDAEQHQGCVEDMRLPDGTLWPIPVCLGLPDGAGLGDGGLVALRGQDGALLGVLEVQ
                     EVFE"
     gene            complement(761..2209)
                     /locus_tag="VE646_05830"
     CDS             complement(761..2209)
                     /locus_tag="VE646_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HZD79540.1"
                     /translation="MVRICADERGGRMEPRPRRLSPLDVSNLRVEDRGRPMHVAGLAI
                     LEGAPLLDASGELRLSALLEHLERRLHLAPALRQVLYRPRLGLGPPVWVDDPGFDIRR
                     HVRARAVPAPGDEEALLRVCSELNEPPLDRSRPLWELWLLTGLAGGEVGVLIRLHHVV
                     ADGIAALALMGALFDASADAAPPVGRPWTPRPVPGARELLGDNLRHRAVAVTRAVSRL
                     RRPAQLVRRLGSTALQVRQLLHEGLAPRVSLNRPVGRRRRLLLGRADLDRVRALAHAH
                     GAKVNDVVLAAVAGGARRLLEHRGELRRDLVLKASVAVSMRRPADQQAAGNVVGIRLA
                     PLPAGDPDPIRRLRAIARATAEGKRRPPLEPGGRFARTWMVQAMSSQRLVNLFTSNLP
                     GPPVRLYLAGARVLELFQVGVVQGNVTVGVGVLSYAGRLNFAIVGDAEAVPDLEVFAE
                     GLSEALEELGSEVRGPSSPPAADRRPGAPSAR"
BASE COUNT          346 a          887 c          805 g          282 t
ORIGIN      
        1 gctcaaagac ctcctggacc tccagcacgc ccagcaacgc cccgtcctga ccgcgcagcg
       61 ccaccaggcc accatccccc aggccggcgc catcgggcag cccaagacag acggggatcg
      121 gccacagcgt cccatccggc agccgcatgt cctccacgca cccctggtgc tgctcggcgt
      181 ccatgaagcc accaagcggg ctgtaggccc ccgtggccag cgcccgaagg tcagcgacct
      241 ccgccgcgct caaggtgagc cgggggatcc gccgcgcggc cagctcctcc gcctcggcca
      301 cggccagttg ccgctgcacc agcctgccac catggggaag gctcccttct gcctccgccg
      361 accaccgccg ggtcgtcgtc accgcctgct ccctcctgct ccgagaccgt gcgctcgcaa
      421 ggcagcgtgc cgggcgcgcc gggcgccgcc ccacctgccg cgggccggag gacgccgcaa
      481 ccgtcgctga tcgccgaacg cgagccccca gcaatcgatc aaaatactcc catacggtgg
      541 cggtcaggct gaccccccca gaggtgcatg ccagacattc ttagcagatc tcggcggcgt
      601 cgattgggcg cctggcggct gtgatgtggc ggggtcctgc gggtcctccg gtaggaggca
      661 tggatgagga tcggcgcgga ccgccttcct ggtcgccggg gagcggcccg gcggcaggct
      721 cgaccaccgg catcgccgcg ccacccgggc acggacctga tcagcgcgct gacggagctc
      781 cgggtcgacg gtcggcggct ggaggactgg acggtcctct cacctcggat cccagttcct
      841 caagcgcctc cgacagcccc tccgcgaaca cctccagatc cggcacggcc tcggcgtcac
      901 cgacgatggc gaagttcagc cgtcccgcat acgagaggac tccgacgccc acggtcacgt
      961 tcccctgcac cacgcccacc tggaacagct ccaggacccg cgcgccggcc aggtacagcc
     1021 gcacgggagg gccggggagg ttgctggtga acaggttgac cagccgctgg gaggacatcg
     1081 cctggaccat ccaggtccgg gcgaaccggc caccgggctc cagcggtggg cgtcgcttgc
     1141 cttccgcggt cgcccgcgcg atcgccctga gccgccggat gggatcgggg tcgccagccg
     1201 gtaggggggc gagcctgatc cccaccacgt tccccgcggc ctgctggtcg gcggggcggc
     1261 gcatggacac ggcgaccgag gccttgagca ccaggtctcg cctgagctcc ccgcggtgct
     1321 cgagcagccg tcgcgccccg ccggccactg ccgccagcac gacgtcgttg accttcgcac
     1381 cgtgagcgtg ggcaagcgct ctgacccgat cgagatccgc tcgcccgagc aggaggcggc
     1441 ggcgcctccc cacggggcgg ttgagcgaca cccgcggcgc caggccctcg tgaagaagct
     1501 ggcggacctg gagagccgtc gagccgagtc gacggacgag ctgggccggg cggcggagcc
     1561 gggatacggc tcgggtcacg gcgacggcgc gatgtcgcag gttgtccccg aggagctcgc
     1621 gagcgccggg aacgggtcgg ggggtccagg gccgtccaac cgggggcgcg gcatccgcgg
     1681 aggcatcgaa cagggcgccc atcaacgcca gggcggcgat gccgtcggcg accacgtggt
     1741 gcagccggat gaggaccccg acctccccgc cggcgagccc ggtcaacagc cacagctccc
     1801 acagcgggcg cgaccggtcc agcggcggct cgttcagctc cgagcagacg cggaggagcg
     1861 cctcctcgtc gccgggggcc gggacggcac gcgcccgtac atgacgtcgg atgtcgaagc
     1921 ccggatcatc gacccacacg ggcggcccca ggccgaggcg tggccggtac aggacctggc
     1981 gcagagcagg cgccaggtgc aggcgccgtt cgaggtgctc cagcagcgcg ctcagccgaa
     2041 gctcgcccga ggcgtccagg agcggagctc cctccaggat ggccagcccc gcgacgtgca
     2101 tgggccggcc gcggtcctcg accctcaggt tgctcacgtc aagcgggctc aaccgccgcg
     2161 gtctcggttc catacggccc ccacgctcgt cggcgcatat cctcaccata ggtgcccacg
     2221 ctggctcccg tcggccgtga cgcaggtcca ccttgcatgc tgaccatggg cgctcacagc
     2281 ccagcctggg ccggccggca cccgggcgct agggccgatg
//
LOCUS       DASQQQ010000142        20144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_149164, whole genome shotgun sequence.
ACCESSION   DASQQQ010000142 DASQQQ010000000
VERSION     DASQQQ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 20144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20144
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149164"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..145)
                     /locus_tag="VE646_05835"
     CDS             complement(<1..145)
                     /locus_tag="VE646_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HZD79541.1"
                     /translation="MSEREVKDALYEQFARLGKAVASPKRIELLDLLCQGERTVEALA
                     REAH"
     gene            280..1581
                     /locus_tag="VE646_05840"
     CDS             280..1581
                     /locus_tag="VE646_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD79542.1"
                     /translation="MRTSTSGQTRLGIGANLAQFSLLVGVEVLVGGMVGQERAIVPLL
                     ASRIFGLHAFSAALSFIVVFGFVKAATNFFAGSLSDRLGRKRVLVAGWLVGIPVPLLV
                     IFAPSWGWVIAANALLGVNQGLSWSDTVIMMVDLAGPGSRGLAMGLNEAAGYGAVALT
                     ALATGVIAQHAGLRPEPFFLGLAYAGLGLGLSALIVRETRGHAALEANTRRPVSAEAG
                     ERQPSTTRVFVMTSLKQRALSACSQAGLVKNLNDGVAWGLFPLLFSHHGLSLSAVGFL
                     VAIYPAIWGFGQLLTGPLSDRLGRKWLIASGMWLQATGLAIIAGTSGFGPWVLGSASL
                     GIGAAMVYPTLLAAVSDVAHPTWRARALGVYRLWRDAGYAIGAIIAGVLADRFGIRAT
                     LWTVGALTAASGLVVAIRMYETLPTTLRAAGSRRGSATSLS"
     gene            complement(1663..2049)
                     /locus_tag="VE646_05845"
     CDS             complement(1663..2049)
                     /locus_tag="VE646_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HZD79543.1"
                     /translation="MRERAKELKAEARANKDRAAGESDVQAKIAEMPEPDRTMAKRLH
                     EIVKASAPSLSPRTWYGMPAYARDGKVVCFFKPASKFNSRYATLGFEEAANLDEGTMW
                     VTSFALTRLTAADEKKISALVKQAVS"
     gene            complement(2224..3120)
                     /locus_tag="VE646_05850"
     CDS             complement(2224..3120)
                     /locus_tag="VE646_05850"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZD79544.1"
                     /translation="MDDTLIREVTIQRTFDAPRDAVWAAWTDGEQLERWFMPHGFTVP
                     ECTVDPRPGGRLHLRVRGPDGAEMSNDGLFEEVDPPQRLVMTTTAFEGRLEVRNTITF
                     EDADGGTELTLHAQVVKAAPDLAAALAGMEEGWLQSFEKLDAVLTGGEVDIMSRAVVA
                     SRLLDAPRQAVWAAYTDPEQLERWWAPPGHTIETQQIDVRPGGIWRSTLRGPHGEFEQ
                     RITYLTLQEPQVLAYLYGDPDQPGHAFTLVELAEQGGKTTVTVTIHFSSAEERRGMVE
                     HGAQAGLEGALDRVAAYVSEHR"
     gene            complement(3105..3527)
                     /locus_tag="VE646_05855"
     CDS             complement(3105..3527)
                     /locus_tag="VE646_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018096111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZD79545.1"
                     /translation="MTAAADTLDRTFAALADPTRRAILERLAAGEATVQELARPFPIS
                     LPAVSKHLKVLERAGLIARGRRAQWRPAELRAEPLHEAADWLEEYRRLWEGRLDRLDA
                     FLKEIRPDPDRERAGYEPTGPDPAGPDPKGDQEPWTTR"
     gene            complement(3711..4556)
                     /locus_tag="VE646_05860"
     CDS             complement(3711..4556)
                     /locus_tag="VE646_05860"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HZD79546.1"
                     /translation="MSTSALFGIKTALALAVLAIGLFGALLPWALGARGLSDRPLALG
                     DTFAGGVLGGAGLVHLLSDGIDRFRAALPAVGYPLALLLAGAGFLMILLIEGVVVAGR
                     EEVHVPGVPADRAAGARHEVGWLPGTGEEHRPTHPLVLLIVLSAHSVILGLALGAQGA
                     VAGALVVFLAIVAHKAVAGFALGMGYRRAGFARNRAAPQVAFFAAMTPLGILAGSLAT
                     VALASHADQLFEAVFDSLGAGTFLYIATLDILRTEFDGPEDRWRKWLAASLGFGLMAL
                     LAVWV"
     gene            complement(4810..4944)
                     /locus_tag="VE646_05865"
     CDS             complement(4810..4944)
                     /locus_tag="VE646_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79547.1"
                     /translation="MPCRIHATAELAFLETESEYVHRYKILKELLSDAESGGGVAHAA
                     "
     gene            complement(5152..5385)
                     /locus_tag="VE646_05870"
     CDS             complement(5152..5385)
                     /locus_tag="VE646_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79548.1"
                     /translation="MADQEHRHEGRVHEHEHVHVTHYLRPDEDWVHLTATHAHEHNHA
                     PLQHTHVSHEEPDKEHEREAHIHDHVSPETSPS"
     gene            complement(5528..6889)
                     /locus_tag="VE646_05875"
     CDS             complement(5528..6889)
                     /locus_tag="VE646_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HZD79549.1"
                     /translation="MSEFIREVSSGRNRFVLRVATIAAIGGFLFGYDTGVIGGALLYI
                     KHDLHATSKLDQQAIVASLLVGAVGGALLGGRLADRLGRRRTIIGAGWVYVAGGLGSA
                     ISMGTWQLVGARLVLGLAVGAASFVTPMFISEIAPRDIRGGTVTFNQLMLTSGILVAY
                     IADWGLKGLPANWRWMLGLAAVPGLALAIGMLFVPPSPRWLAEQGRGNEARQVLQRVH
                     GKGDVQEEFAQIEQAARQRAGHLALLRPSLRPVVLVGVGLAAFQQLVGINTVIYYAPT
                     ILSFTGIGAGSALTKTVFIGVINVAFTVVAILLLDRVGRRPLLLLGTSGLVIALAALG
                     LFFRLGWLQQHASVMALGALLLYIAAFAVGLGPVFWLMISEIYPLGIRSAAESTASVV
                     NWGTNFAVSFTFLTVVGALGRPGAFWLYAAIGLVTIWFILTRVPETRGRSLEEIQRQL
                     GAS"
     gene            7392..9275
                     /locus_tag="VE646_05880"
     CDS             7392..9275
                     /locus_tag="VE646_05880"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HZD79550.1"
                     /translation="MPAATLSGQTTVTVTNTPNSGSVMVTWSPSGGSTGTLITEFGPS
                     PVTNDYSFAWPTQKYLDASGVLRVYWGSTSNAPVEVALALANGNTSDIQHSPDDWQSF
                     LPDPTWNGASDPAVIAVGDGASNEPASNGLADSLAAANPPLFLYLGDIYQDGTFTENL
                     NHYGRNSMDGGAGTLWGQMGTVTQPALGDHEKNNLPAWRDYFHGRPNYTAFTFGNVLF
                     LDLSSSGTPMGKGKPQYDFVQSVLQSNTAPCVITYFQNPVINKHGIKDRRVDMWKLIT
                     DNGGDLVLTGNSHTMAQYKPLNDQLALPSPGDATMVELIDGAGGHALGGTFPSDPKLE
                     WSLGKTPGALHITLQGAAEGGTPTSLGWSYRDTNGNALHSGTRDCGGSTTPPPAGPAI
                     TGFSPTSGPVGTEVTVEGSSFTDATDVAFAGTSVGTGNFTVDSDSQITTAVPSGATSG
                     PISVTTPDGTATSSDSFTVTAPGSALTFTPDADTWVQSSRPDKNFGSRPFVKVDGSPE
                     RHALLRFTVSGLGSSAPAGAMLRLYCTNASQDGGSLYRVTDQTWQESTVTWNTQPPTD
                     STPFASLGRVTADTWVEVDVSSLVTGDGTYSLQIVTPSKNLSAYTSKEGTAGFAAQLV
                     VTP"
     gene            9336..9503
                     /locus_tag="VE646_05885"
     CDS             9336..9503
                     /locus_tag="VE646_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79551.1"
                     /translation="MFFPRSPLLPFGVPRDLRRLLVFLEGWLGLAGDRAAVPDTGQVI
                     EAGLPTRTPFA"
     gene            9537..11801
                     /locus_tag="VE646_05890"
     CDS             9537..11801
                     /locus_tag="VE646_05890"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZD79552.1"
                     /translation="MQQRAEPGEPAPPRPDRGPGRDHRPSRPARAYAWFSTHLRYLIV
                     AAWIAAPIACILWLPSLESVSTNDVGELIPHDIPAYRAEQLSARVFGFPLTAETVVVQ
                     RNPNGLSASEQAHIVRTDIALTQGNIQGYDQIRAAIPVLNTLGLFPGSREHSTTAVTY
                     LSMTGTRSIGDQTVMAHRFARQELGSNVVGVTGPVPARVEQGRLISQWLPWVEIATVA
                     MIALVVGFAFRSIGAPLAVLASAAVAYVVATRLVAWAGTHFQVAIPPDLEPVLVVLLL
                     GVATDYSVFFLSGMRDRLADGSARLDAAKRTVTDVAPIIFAAGSIVTAGTAALLAARS
                     DLIRAFGPTLAITVFVTMAVSISLIPALIAIFGRVLYWPHRPEPSFEQKRLARLRSWL
                     ARVRTAKPVALVISVVAVVALSVAGWEVLHIRMGLPLVRALPNSTEAARAETAAAKGF
                     APGILSPTAVILQAQGIGTQTDALARLEQELRQQPDVAGVLGPADTVVGTALNVLVSE
                     SQDAVRYVLVLNSDPLAGPAVDALNGLRDRMSALLDRAGLSGITVSYAGNTAIVTDTI
                     RSTISDLWRVGIATLAVDLLLLMLFLRSLVAPFYLMVASLLALAASLGLTTYFFQSFL
                     GSGGITYFVPFVAAVLLVSLGADYNVFLVGRIWEEAGRRPLREAIITATPRASRAINL
                     AGLTLALSFGFLALVPLDQFREVAFAMGVGILLDAFVVRPVLVPALISLFGYLSGWPG
                     KALRRGRELEEASS"
     gene            12164..12592
                     /locus_tag="VE646_05895"
     CDS             12164..12592
                     /locus_tag="VE646_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79553.1"
                     /translation="MASDGAKVVGDGGGRLIQIEGSFIVVRGFEISDADILVWIQHAH
                     GVRLARNLLHDAGSECVRIKYLASDNVLAHNRIVRNRSHVPAECVDVKEGSARNPGGA
                     QQLYRRPGPGQRRLLIARTAHALHRERLSGHGGRRYPPRW"
     gene            complement(12803..14323)
                     /locus_tag="VE646_05900"
     CDS             complement(12803..14323)
                     /locus_tag="VE646_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZD79554.1"
                     /translation="MRPYNPKPLDVVRDPGKLAGLHRRTLRLSTDRIDLMRGATVLRS
                     VPFDARSTVSFSAVAAAVGPGPWVKDLGDGQLLLKAAFAQGPGTSVRFGGDGTDTIRL
                     AARPDVFIGGVQARATFQDVLVTSWNVSAQQVDRDYRGDRPFILYEGGSVLDIVGSEI
                     AYLGSDRSMAYGTSWRLGATGSMTDSDVHDNFFGVYSFRARDLRLEGNRIHDNVYYGV
                     DPHDYTKGLDIRGNEVYGNGDHGIVLSVGVTDGVIAQNHVHDNDGNGIVMDERSDGNL
                     IEGNRSERNRGDGIVVLGSSRVVVRGNVISDNRVGVRLNHDGEHNVVRSNTIQGNREG
                     VEVYDGAVDTLFEANRISSSSEAGMVLEEVGSVSRDDRISDGPVGIEVRGPATIEGSA
                     TRGVERGIVVTSRGIAEIDGVDLEASDAAVELDRGAIARVRRSRLAAPDPFTGAQPRL
                     AVANDIAHPWWSLPWFAIAGLSFVLLALVLQAVHRTRNIGMRRHRSRAPAGVSNAR"
     gene            complement(14512..15897)
                     /locus_tag="VE646_05905"
     CDS             complement(14512..15897)
                     /locus_tag="VE646_05905"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZD79555.1"
                     /translation="MRVVWRTLLALVLAGGLAAVAWSLLARHRVQTPVRLEVDQATGV
                     VHLTRGQTDLPGLQMALQRGADGSILRRQGEAWLLTGTLVIDTGASLELSGATLRLNS
                     TPDQEVGLEARGGDLTIVDSMVTSWDPSRGTADGDVRDGRAWVLARDGSSMIVRDSTM
                     RMLGYDAYERYGVSWRTAGTGGSVDGSTFAGNYYGLYTFGAEPMRITDSVIKDSLSYG
                     LDLHTGSDGFLIQGDRFLHNGKHGFILAVDCTNAVVKDNLAVGNAQHGFVVFQGSDHA
                     TLEGNDAHGNGGSGIDVSGSKDVRVRGNAVYENRNGIVIHDRSSDIAVEGNRVAGNGS
                     DGVVLSSGASSVTLTGNLVDFNRRSGLYLDDGTVRVQGDNRILDNTVGVWVSGGMEEA
                     TITGNRIGGSTDDGVHVTDLTPAPVIRGNRITESGKAAFSGSSKAQVERFVAENTLRD
                     NDAAMRVRGPS"
     gene            complement(15894..17219)
                     /locus_tag="VE646_05910"
     CDS             complement(15894..17219)
                     /locus_tag="VE646_05910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZD79556.1"
                     /translation="MDWIGSLAFLVPLGILGVIRWTSWLIRRLPAVLYRSVENDHREP
                     LSIVVPVYQEDPQTFRYALESWLRNDVEEIILVIDHTDRTCIDIASEYPVRVIVTPVP
                     GKRPALKTGWEATSTPLVALVDSDTIWASDVAQLVCMPFADPKVGGVATRQNVANPRT
                     PWQHLNDMYLDYRYFDEVAAQTVRGRAISCISGRTAVYRRELLLRHADRFMNETFLGV
                     RCMSGDDKRLTTLILEGGHLTVLQRSARVWSSFPPDALGFVKQRLRWSRNTWRSDLRA
                     LKDGWVWHHPFLAFSMIDKAVSSFTLLVSPTIMVLALIAGNELVAGLLGAWWLLSRSA
                     KMLPHLERRPRHAVTMMPLFIVTSFVMALVKISALFTIRRQRWLTRDVEVSARTGEVE
                     RSGRNGSVPSPTSVAAALPPPPPPRAPAALPPLPPPREHPDLFAWKGKR"
     gene            complement(17219..18208)
                     /locus_tag="VE646_05915"
     CDS             complement(17219..18208)
                     /locus_tag="VE646_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HZD79557.1"
                     /translation="MAHVVIVGSGVVGTATGKGFARHGHRVSFVDIDPRRIDALRSEG
                     FGATDHVRLPSEPSIVFLTLPTPNDGHRWDLGPFRRGAEQVAEAMKDSHALHTVVVRS
                     TVPPGTTDGLVRPILEEATGGRQGERFSLAANPEFLRAACALEDFLNPWMTVVASRSK
                     RTLERMRRLFEPFGGEIHTFGDPRVAELVKCAHNLFNATKISFWNEIWRVGNRLGMDV
                     ADVARIVSRSAEGSYNPEYGINAGAPYGGACLPKDTKGFLGFARHVGVEMPLLQAVDE
                     VNEQVAADGQGITPDRHEAADGPTTVPSAPPVAGTIEPDADPSADGQAVRRAS"
     gene            complement(18263..19393)
                     /locus_tag="VE646_05920"
     CDS             complement(18263..19393)
                     /locus_tag="VE646_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HZD79558.1"
                     /translation="MLPTNPDPLRHAPWRAVVLAGGKGTRLRPLTESRPKPLVPFMGA
                     PYAAGLLRRLAQAGCGRATFLVGPDAGPFAPIQDLGRAAGIRVDLVTEERPLDTAGAA
                     RRLLAGTGERSVVVLNGDILSDVRYERLIEAHRRSRSVATLALMRVEDPSSYGVVVRD
                     DAGRVRRFLEKPAPGTTAHRTVNAGAYVLDASAFDLLPGDGSLSFERDVFPALVDEGA
                     LVTGVIPHAYWQDLGTPERYRAGHRAVLEGRCAWPWAPGLRRIGPLAAVHRDAAVDRA
                     ASLAGPVVVGAGCAIEPGARIGDAVLFDEAAVEGSASVSQAILGRGARVRSGIRLDPG
                     SVLADGAVVAGPVTAPSREAGPALLQDPPMSADDGVRMTDDG"
     gene            19567..>20144
                     /locus_tag="VE646_05925"
     CDS             19567..>20144
                     /locus_tag="VE646_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HZD79559.1"
                     /translation="MARAIWSGAVSFGLVNVPVRLYSATESKDVRFHEFQQGTGRRIR
                     HERVAEGTDQEVPYEDVVKGYEYADGQYVTLTQEELEAAQPERTRGIAIEDFVELADV
                     DPIFFQKTYYLDPGPGAEKPYALLLRAMEETGRVGIARFVFRTKEHLAAIRPMDGLLV
                     LETMFFADEVRDVGDIDGVPAQADLDQREVDMA"
BASE COUNT         2958 a         7303 c         6814 g         3069 t
ORIGIN      
        1 tgtgcgcctc cctcgccaac gcttccacgg tccgctcacc ctgacataga agatccagca
       61 gctcgatccg cttggggctg gccacggcct tgcccagccg cgcgaactgc tcgtacagcg
      121 cgtccttcac ctcgcgttca ctcacttgac gagcgtcctc caatattcca atactctttg
      181 gatgaatact aatgctctgg gaggggcgtc acaaggatcc ggtggtgctc gcggcccagg
      241 cgagcgatcc cgccgaacgg aacgcttcct ccaacggcca tgaggacgtc cacttccggg
      301 cagacccgtc tggggatcgg ggccaacctc gcccagttct ccctcctggt gggtgtggag
      361 gtcctggtgg gcggcatggt cggccaagag cgtgccatcg tccccctcct cgccagccgg
      421 atcttcggcc tccatgcgtt cagcgccgcc ctctcgttca tcgtggtgtt cggcttcgtg
      481 aaggcggcga ccaacttctt cgccgggtcc ctctcggatc ggttggggcg caagcgcgtg
      541 ctcgtggcgg gctggctggt cgggatcccg gtgcccctgc tcgtgatctt cgccccgtcc
      601 tggggctggg tgatcgcggc gaacgcgctc ctcggggtga accaggggct cagttggtcg
      661 gacacggtga tcatgatggt cgacctggcc ggcccaggga gcaggggcct ggccatgggg
      721 ctcaacgaag ccgccgggta cggagcggtc gcgctgaccg ctctggcgac cggcgtgatc
      781 gcccagcacg ccgggctgcg gccggagccg ttcttcctcg ggctcgccta cgccgggctg
      841 gggctcggcc tctcggcgct gatcgttcgc gagacgaggg gccacgccgc cctggaggcg
      901 aacacgcgcc ggccggtctc ggccgaggcc ggcgagcggc agccgtccac gacccgcgtc
      961 ttcgtcatga ccagtctcaa gcagcgagcg ctctcggcgt gcagccaggc cggcctggtc
     1021 aagaacctca acgacggggt ggcttggggc ctgttcccgc tgctgttctc gcaccacggg
     1081 ctctccctca gcgccgtcgg gttcctcgtg gcgatctacc cggcgatctg gggcttcggc
     1141 cagctcctga cgggacccct gtccgatcgt ctcggccgca agtggctcat cgcgtccggg
     1201 atgtggctcc aggcgaccgg gctcgcgatc atcgccggga cctcgggctt cggtccgtgg
     1261 gtcctgggct ccgcatcgct cggcatcggg gccgcgatgg tctacccaac cttgctcgcg
     1321 gcggtgagcg acgtcgccca cccgacctgg cgggcgaggg cgctcggcgt gtaccggctc
     1381 tggcgtgacg cggggtacgc gatcggagcg atcatcgcgg gggtactcgc ggaccggttc
     1441 gggatcaggg ccaccttgtg gacggtcggt gccctgaccg ccgcctcggg gttggtggtg
     1501 gcgatccgga tgtacgagac cctacccacc acgctgcgag ccgcaggttc gcggaggggg
     1561 tccgcgacct cgttgtcata gcgcgatcgc ggggacccga cgaccggcgc tcgtgacccg
     1621 caacggcgag atctcgcacc cgtggccttc gggacccggt cctcagctca ccgcctgctt
     1681 cacgagggcg gagatcttct tctcgtcggc ggcggtcaac cgcgtcagcg cgaacgaggt
     1741 cacccacatg gtgccttcgt ccaggttcgc cgcttcctcg aagccgagcg tcgcgtacct
     1801 cgagttgaac ttggacgcag gcttgaagaa gcagacgacc ttgccgtccc tggcgtaggc
     1861 gggcatcccg taccaggtcc tcggcgacag ggagggcgcg ctggccttca cgatctcatg
     1921 gagccgcttc gccatggtgc gatccggttc gggcatctcg gcgatcttcg cctgcacatc
     1981 gctctccccg gcggcccggt ccttgttggc acgcgcttcg gccttcagct ctttggcgcg
     2041 ctccctcatc gcggctcgtt cctcgtccgt gaaccccccg gaccccctgc cgcgagagct
     2101 cgtggcggac ccctgggctc cctttttcgc tggttccctc tcagccatcg cggacctcct
     2161 ccttgcatcc gatcgggatg aacccgtgca gccacatcgt ggcgccggag aactccctcg
     2221 tcatcagcga tgctccgaga cgtaggcggc gacccggtcg agggcgccct ccagaccagc
     2281 ctgcgccccg tgctccacca tgccccgacg ctcctcggcc gaagagaagt ggatcgtgac
     2341 cgtgacggtc gtcttcccgc cctgctcggc gagctccacg agcgtgaacg cgtggcccgg
     2401 ctggtccggg tcgccgtaga ggtaggcgag cacctgcggc tcctgcagcg tcagatacgt
     2461 gatcctctgc tcgaactcgc cgtgtgggcc tcggagcgtg ctgcgccaga tgccgcccgg
     2521 ccgcacgtcg atctgctgcg tctcgatcgt gtgccccggc ggcgcccacc agcgctcgag
     2581 ctgttcgggg tcggtgtagg cggcccacac cgcctggcga ggcgcgtcga gcaggcggga
     2641 ggcgaccacg gcccgagaca tgatgtcgac ctcgcccccg gtgaggaccg catcgagctt
     2701 ctcgaagctc tggagccagc cctcctccat cccggccagg gcggcggcga ggtccggtgc
     2761 ggccttcacg acctgggcgt ggagcgtcag ctcggtgccg ccgtcggcgt cctcgaaggt
     2821 gatggtgttg cgcacctcga gtcgcccctc gaacgcggta gtcgtcatga ccagccgctg
     2881 cggcgggtcg acctcctcga agaggccgtc gttggacatc tcggcgccgt cgggaccgcg
     2941 cacgcgcagg tggagccggc cgcccggccg cgggtccacg gtgcactcgg ggacggtgaa
     3001 gccgtgcggc atgaaccaac gctcgagctg ctcgccgtcg gtccaggccg cccacacggc
     3061 gtcgcgcggc gcgtcgaagg tgcgctggat cgtgacctcg cggatcagcg tgtcgtccat
     3121 ggctcctgat ctcccttcgg gtcgggtccg gctgggtccg ggccggtagg ttcgtagccg
     3181 gcgcgttccc ggtcggggtc gggtcggatc tccttgagga aggcatcgag gcggtcgagt
     3241 cgcccctccc acagtcggcg atattcctcg agccagtccg ccgcctcgtg cagcggctcg
     3301 gcccgaagct cggcggggcg ccactgggcg cgccggccgc gggcgatcag gccggcgcgc
     3361 tcgagcacct tgaggtgctt ggagacggcc ggaaggctga tggggaaggg ccgcgcgagc
     3421 tcctggacgg ttgcctcacc cgcggccagg cgctcgagga tcgcccgccg ggtgggatcg
     3481 gcgagggcgg cgaaggtgcg atcgagggta tcggcggcgg cggtcatctt ggtgaacctc
     3541 tcggttattt atcttcctgg ttatataccg caccgttcgg tcgtgtcaag ctccgtccca
     3601 ccgcaacgga ccctgaggcc accggggtac gccaagtggc tcgcccgacc ggtggtggtg
     3661 ctgggccccc gcccgacccc gacccggggg accgagcggt acccaaggac tcagacccac
     3721 accgccagca acgccatcag cccgaaccca agcgaggcgg ccagccactt ccgccagcga
     3781 tcctcggggc cgtcgaactc ggtgcggagg atgtccagcg tggcgatgta gaggaaggtg
     3841 cctgccccga gggagtcgaa cacggcctcg aacagctggt cggcgtggct ggccagcgcc
     3901 accgtggcga ggcttccggc caggatcccg agaggcgtca tggccgcgaa gaaggcgacc
     3961 tgcggcgcgg cccggtttcg ggcgaagccc gcgcgccgat agcccatgcc cagggcgaac
     4021 cccgccaccg ccttgtgcgc cacgatggcc aggaacacga ccagcgctcc ggccactgcg
     4081 ccctgggccc cgagcgccag acccaggatg accgagtgcg cggacagcac gatcagcagc
     4141 accagggggt gggtgggccg gtgctcctcc cccgtcccgg gcagccatcc gacctcgtgc
     4201 cgagcgcccg ccgcccgatc agcggggacc cccggcacgt ggacctcctc gcggccggcc
     4261 acgaccacgc cctcgatcag caggatcatc aggaacccgg ccccggccag caggagggcg
     4321 agcgggtacc ccaccgcggg caaggcggcc cggaacctgt cgatcccgtc gctcagcaga
     4381 tggacgaggc cggccccacc cagcacgccc ccggcgaacg tgtcacccag cgccaggggc
     4441 cggtcgctca ggccccgcgc cccgagtgcc cacggcagca gggccccgaa gagcccgatc
     4501 gccaatacgg ccagggccag ggcggtcttg atcccgaaca gagcggaggt gctcacgtcg
     4561 agccatcgta gtgtggtcct gcccggagac cgcagcgcag gccggtcgcg ttcgcgatcc
     4621 acccacagcg ggtggacgga cctcccgggc tcccggcacg aagacccgac cgcgtcacgg
     4681 gccatcgccc gcgcagcccg gcgagaccga gattggggcg actaccgcgc ttgaccgatc
     4741 cgctcccggg ccacggcgag cccgggctcc agctgacgcc ggcccgtagt gacggtgatg
     4801 gcgacaggct caggcggcat gggccacccc acctccgctc tccgcgtcgg agagcagctc
     4861 cttgaggatc ttgtaccggt ggacgtactc ggactcggtc tccaggaacg ccagctcggc
     4921 ggtggcatgg atccggcagg gcaaccctgg tcggagcgct tcctggccgc accggtccgc
     4981 gcccgtccac cgtccacaag aacccggcga cggtctcgcc ctccttcgcc tccgtctccc
     5041 gtccatccag cccccctatc ctcggatgat cgccaccggg agcgggcggg tccggtgggt
     5101 ccgtcggtcc ttcagccctc ctcttcgtga ggcgggtggg tcaccgcgat gtcacgaggg
     5161 cgaggtctcc ggactgacgt gatcgtggat gtgggcctcg cgctcgtgct ccttgtccgg
     5221 ctcctcgtgt gagacgtgtg tgtgctgcag gggcgcgtgg ttgtgctcgt gcgcatgggt
     5281 ggccgtcaag tgcacccagt cctcgtccgg tcggaggtag tgggtcacgt ggacgtgctc
     5341 gtgctcgtgc accctccctt cgtggcggtg ctcctgatct gccatggccc cctcctttcg
     5401 ggttcccgtc tcgtccctac ccgccgatcg gctccaccac cgccgcggtc gggtcctctg
     5461 gcgagtcaca tccagctcgg tgccatgagc ccgaggtccg agtgggccga tgaggggatc
     5521 ggctcggtca cgacgcgccg agctgccgct ggatctcctc caggctccgt ccacgcgtct
     5581 cgggcaccct ggtcaggatg aaccagatcg tgaccagccc gatcgccgcg tacagccaga
     5641 acgctcccgg tcgacccagt gcccccacca ccgtgaggaa ggtgaaggac acggcgaagt
     5701 tcgtgcccca gttcaccacc gaggcggtgc tctcggcggc gctgcggatg cccaatggat
     5761 agatttccga gatcatcagc cagaacaccg gtcccagccc caccgcgaag gccgcgatgt
     5821 acagcagcag cgctccaagg gccatcaccg acgcgtgctg ctgcagccag cccagccgga
     5881 agaacagacc cagcgcggcc agcgcgatca ccagccccga agtgccaagc agcagcagcg
     5941 ggcggcggcc cacccggtcc agcagcagga tcgccaccac cgtgaaggcc acgttgatca
     6001 ccccgatgaa cacggtcttg gtgagcgcgc tgccggcacc gatcccggtg aaggacagga
     6061 tggtgggggc gtagtagatc accgtgttga tccccaccag ctgctggaac gcggccagcc
     6121 ccacccccac caggaccacc ggccgcagcg atggccggag cagggccagg tgcccggccc
     6181 gctgccgggc cgcctgctcg atctgcgcga actcctcctg cacgtccccc ttgccgtgga
     6241 cccgctgcag cacctgccgg gcctcgttcc cccgcccctg ctcggccagc caccgagggc
     6301 tgggcgggac gaagagcatc ccgatcgcca gggccagtcc ggggacggcg gccagcccga
     6361 gcatccaccg ccagttcgcc ggcaaaccct tcagccccca gtcggcgatg taggcgacca
     6421 ggatgccgct ggtcagcatc agctggttga acgtcacggt gccgccccgg atgtcccggg
     6481 gggcgatctc ggagatgaac atcggggtca cgaacgaggc cgcccccacc gccaacccca
     6541 gcaccagtct ggcccccacc agttgccagg tgcccatcga gatcgccgag cccaggccgc
     6601 cggccacgta gacccagccc gccccgatga tcgtgcgccg gcgtcccagc cgatcggcca
     6661 gccgccctcc gagcagcgcc ccgcccaccg ctcccaccag caaggaggcc acgatggcct
     6721 gctgatccag cttcgaggtg gcgtgcaggt cgtgcttgat gtacaggagc gcgccgccga
     6781 tcacgccggt gtcgtagccg aacaggaacc cgccgatcgc cgcgatcgtc gccacgcgga
     6841 gcacgaaccg gttccggccg gacgagacct ccctgatgaa ctcgctcatg gaacgccctg
     6901 gcacccctac ccgaggcgag cggtcgggaa gcaggtctgc acgagcgcgc ggcccgaagg
     6961 atcgggttgg ggcctccagg ggccaggggc cggggtcccg gccaggtcgt tgccgcggcc
     7021 tcccccctca gcactcgcca cgtcgcgagc aggtacccgc gtccggtctt cgcccgaggc
     7081 gatgctgtac ggatctttgg tgcaggtgag ggattcgttc ccgggccgga tgtccaagga
     7141 tggggtccga cccggaaccc gcggtggcac cgcgtcgtcg ggtccgcctc gatgtgggtg
     7201 gtcctccccg gcgcgtccct gccgttcgac ccagccgagg gaggcgaacg atgcacccac
     7261 agctcatcat ccgccggctc gtgcgcgtcg gcccggtcgt ggccatcgtc gcttctctgc
     7321 tgacggttgg cccgtccgcg acggcggctc ccgagtgtgg gagctccggc agtcacacga
     7381 tctgcgtatc catgccggct gcgaccctat cgggacagac cacggtgacc gtgacgaaca
     7441 cgccgaacag cggctcggtg atggtgacgt ggagcccgtc gggcgggtcc accggcacgc
     7501 tgatcacgga gttcgggccc tccccggtca cgaacgacta ctccttcgcc tggcccacgc
     7561 agaagtacct cgatgcgtcc ggcgtcctgc gtgtgtactg gggctcgacg tcgaacgccc
     7621 cggtcgaggt ggcgctcgcg ctggcgaacg ggaacacctc ggacatccag cacagccccg
     7681 atgactggca gagcttcctg ccggacccga cgtggaacgg cgcctccgat cccgcggtga
     7741 tcgcggtcgg ggacggcgcg agcaacgagc cggcctccaa cggcctggcc gacagcctgg
     7801 ccgcggcgaa cccgcccctc ttcctgtacc tgggggacat ctaccaggac ggcacgttca
     7861 ccgagaacct gaaccactac ggacggaact cgatggacgg tggcgccggg accctgtggg
     7921 gtcagatggg cacggtcacc caaccggccc tcggcgacca cgagaagaac aacctgcccg
     7981 cgtggcgcga ctacttccac ggacgcccga actacacggc cttcaccttc ggcaacgtgc
     8041 tgttcctgga cctgtcctcc tcgggcacgc cgatgggcaa gggcaagcct caatacgact
     8101 tcgtgcagtc ggtcctgcag agcaacaccg ccccctgcgt gatcacctac ttccagaacc
     8161 cggtgatcaa caagcacggg atcaaggacc gacgcgtgga catgtggaag ctgatcacgg
     8221 acaacggcgg cgacctggtc ctgaccggta actcgcacac catggctcaa tacaagcccc
     8281 tgaacgacca gctggcgctg ccctcgccgg gcgacgccac catggtcgag ctgatcgacg
     8341 gcgccggcgg ccacgccttg ggtggcacct tccccagcga cccgaagctc gagtggtcgc
     8401 tgggcaagac ccccggcgcc ctgcacatca ccctgcaggg ggcggccgag ggagggaccc
     8461 cgaccagcct tggctggagc taccgggaca ccaacgggaa cgcgctgcac tcgggcaccc
     8521 gggattgcgg cggctcgacg accccacctc ccgccggccc cgccatcacg gggttctcgc
     8581 ccacgtccgg cccggtggga accgaggtga cggtcgaggg ctcgtcgttc accgatgcca
     8641 ccgacgtcgc cttcgcgggc accagcgtgg gcaccgggaa cttcacggtg gactcggact
     8701 cgcagatcac gaccgccgtg ccgagcggcg cgaccagcgg ccccatctcg gtgaccacgc
     8761 cggacggcac ggccaccagc tcggacagct tcacggtgac cgcgcccggc tccgccctga
     8821 cgttcacacc cgacgcggac acctgggtcc agtcgagtcg gcccgacaag aacttcggat
     8881 cccggccctt cgtgaaggtc gacggctcac cggaacggca cgcgctgctc cggttcaccg
     8941 tctcggggct cggctccagc gccccggccg gcgcgatgct ccgcctgtac tgcaccaacg
     9001 cgtcgcagga cggcgggagc ctgtaccggg tgacggacca gacctggcag gagtccacgg
     9061 tgacgtggaa cacgcagccg ccgaccgact ccacgccgtt cgcctcgctg ggacgggtga
     9121 cggcggacac gtgggtcgag gtcgacgtct cctcgctcgt gaccggggat gggacctaca
     9181 gcctgcagat cgtgacaccg tcgaagaacc tgtccgccta cacgtcgaag gagggcacgg
     9241 cggggttcgc cgcccagctg gtggtgacgc cctgagcacc ccgcgatcgt cgttggcgga
     9301 ggttcgatcg gaggggcgcc gggaacgtta gctccgtgtt cttcccccgt tcgcccctgc
     9361 tgccgttcgg cgtgccgcgc gatctgcgcc ggctcctcgt gttcctggaa ggatggctgg
     9421 gcctcgctgg ggaccgtgcc gccgtccctg ataccggcca ggtcatcgag gccggccttc
     9481 cgacgcggac gcccttcgcg tgaaccggag cggagacccg ggtatggatg ctctcgatgc
     9541 agcagcgcgc tgagccgggg gaaccggcac caccgaggcc cgaccgcggg ccgggccgcg
     9601 accaccggcc gagccggccg gcccgcgcct acgcatggtt ctcgacgcat ctccggtacc
     9661 tgatcgtggc ggcctggatc gccgcaccca tcgcctgtat cctctggctg ccctccctcg
     9721 aatcggtctc gacgaacgac gtgggtgagc tgatccccca cgacatcccc gcttaccggg
     9781 ccgagcagct gtcggcccgg gtcttcggct tccccctgac cgccgagacc gtcgtggtgc
     9841 agcggaaccc gaacgggctc tcggcctcgg agcaggccca catcgtccgg accgacatcg
     9901 ccctcaccca aggtaacatc cagggctacg accagatccg cgccgcgatc cccgtcctga
     9961 acaccctggg cctgttcccc ggatcgcgcg agcactcgac caccgccgtg acctacctgt
    10021 cgatgaccgg gacccggtcg atcggggacc agaccgtcat ggcccatcgg ttcgcgcgcc
    10081 aggagctcgg gagcaacgtg gtcggtgtca ccggaccggt tcccgcccgg gtcgaacagg
    10141 gacggttgat atcgcagtgg ctgccgtggg tcgagatcgc cacggtggcg atgatcgccc
    10201 tggtggtggg gttcgccttc cggtcgatcg gcgccccgct cgccgtgctg gcctcggccg
    10261 ccgtcgccta cgtcgtggcc acccgcctgg tggcctgggc ggggacccac ttccaggtgg
    10321 ccatcccacc ggacctggag ccggtgctgg tggtcctgct cctcggcgtg gccacggatt
    10381 actcggtctt cttcctctcc gggatgcgcg accggctcgc cgacgggagc gcccggctgg
    10441 atgcggcgaa gcggaccgtc accgacgtcg cgcccatcat cttcgcggcc gggtccatcg
    10501 tgacggccgg gaccgccgcg ctcctcgcgg cccgatccga cctgatccgg gcgttcggac
    10561 cgaccctggc catcaccgtc ttcgtgacga tggccgtgtc gatctcgctg atcccggccc
    10621 tcatcgccat cttcggacgg gtcctgtact ggccacaccg gcccgagccg tcgttcgagc
    10681 agaagcgcct ggcccgcttg cgctcctggc tggctcgggt gcgcacggcc aagccggtcg
    10741 cgctggtcat ctcggtggtg gcggtggttg cgttgtcggt ggccgggtgg gaggtgctcc
    10801 acatccggat ggggttgccg ctggtccggg ccctgccgaa ctcgacggag gcggccaggg
    10861 cggaaaccgc tgcggcgaag ggcttcgccc ccggcatcct gtcccccacc gccgtgatcc
    10921 tccaggctca ggggatcggg acgcagaccg acgccctggc ccggctcgag caggagctgc
    10981 gtcagcagcc cgacgtcgcc ggcgtgctcg gtcccgcgga caccgtggtg gggacggcgc
    11041 tgaacgtcct ggtgtccgag tcccaggacg cggtccgcta cgtcttggtg ctgaactccg
    11101 atccgctggc cggtcccgcg gtcgacgccc tgaacggtct tcgagaccgg atgtccgccc
    11161 tgctcgatcg agcggggctc agcggcatca ccgtctcgta cgcgggcaac accgcgatcg
    11221 tgaccgacac gatccgctcc accatcagcg atctgtggcg ggtggggatc gccaccctgg
    11281 ccgtggatct cctgctgctg atgctcttcc tccgatcgct ggtggcgccc ttctacctga
    11341 tggtggccag cctgctcgcc ctggccgcgt ccttgggcct caccacgtac ttcttccagt
    11401 ccttcctcgg cagcgggggc atcacctact tcgtgccgtt cgtggcggcc gtgctcctgg
    11461 tctcgctggg cgcggactac aacgtcttcc tggtgggccg gatctgggag gaggccggtc
    11521 gacgaccgct gcgcgaggcc atcatcaccg ccacgccgag agcctcgcgg gcgatcaacc
    11581 tcgcgggcct cacgctcgcg ctgagcttcg gcttcctggc gctcgtgccg ctcgatcagt
    11641 tccgggaggt ggccttcgcc atgggcgtgg gcatcctgct ggacgccttc gtggtccggc
    11701 cggtcctggt tccggcgttg atctcactgt tcggctacct cagcggatgg ccgggcaagg
    11761 cgctgcgccg gggccgggag ctggaggagg cgagcagctg atgtgacccc ccatcctcct
    11821 cagggagccg gtgcgtatga cctggggtgg ttccggttcc ggggaacggg gggtggaacg
    11881 atgaggggca agagatgcgt ggcgtgggcg ctcgccgcat cgctggtgcc gtggccggcg
    11941 ccgaccatcc ggggctgggc ggccggctcg acgaccgggc ggacgatcca cgtctccgcc
    12001 gccgcagggg ctgggggcga tggttcgtcc acccatccct tctcgagcat ccaagccggg
    12061 atcggggggc ccgcgcaggg gatacggtcg ccgtggcccg ggaacctatc gggaagcgat
    12121 ccggatcgat cggtcgggac gtcgcggctc gccgatccgc atcgtggcga gcgacggagc
    12181 caaggtcgtc ggtgacggcg gtggccggct catccagatc gagggatcct tcatcgtggt
    12241 gcggggtttc gagatctccg atgcggacat cctcgtatgg atccagcacg cgcacggtgt
    12301 gcggctggcc cggaacctcc tgcacgatgc ggggagcgaa tgcgttcgga tcaagtacct
    12361 ggccagcgac aacgtgctcg cacacaaccg gatcgtccgg aaccgctccc acgtgccggc
    12421 cgagtgcgtg gacgtcaagg aggggagcgc gcggaaccct ggtggcgcac aacagctgta
    12481 ccggcggcca ggacccggac agcggaggct tctcatcgcg cggacggcgc acgcgcttca
    12541 tcgggaacgt ctctcagggc atggcgggcg ccggtatccg cctcggtggt gatgcggtca
    12601 cggacggcat gtggaacctg gtcatcgata atgtcttgcg tgacaacgcg ggctacgcgg
    12661 tgaagatcat gcggaccccg cagggcctca tctgcgggaa ccacctcgtt cgcaaccggt
    12721 cacgagcctc gaacgaggca gtctatcggc ccggacggcc ctgcccggtc gctgaccggc
    12781 cgcggccctc ctcgtgcggc gttcagcgcg cgttgctgac cccggccggg gcccgcgagc
    12841 ggtggcgccg catgccgatg ttccgggtac gatgcaccgc ctgcaggacc agggcgagca
    12901 gcacgaacga gagcccggcg atcgcgaacc agggtagcga ccaccacggg tgcgcgatgt
    12961 cattggccac cgccagccgg ggttgggctc cggtgaaggg atccggggcg gccagcctgg
    13021 agcgacggac ccgggcgatc gctccccgat ccagttcgac ggccgcgtcg ctggcctcca
    13081 ggtccacccc gtcgatctcc gcgatacccc tcgaggtcac gacgatgccc cgctccacgc
    13141 cccgcgtcgc ggacccctcg atcgtggccg gaccccggac ctcgatgccc acgggcccat
    13201 cgctgatccg gtcgtcgcgg ctcaccgagc ccacctcctc caggaccatc ccggcctcgg
    13261 aggagctcga gatccggttg gcctcgaaca gcgtatccac ggccccgtcg tagacctcca
    13321 ccccttcccg gttgccctgg atcgtgttgg accggaccac gttgtgttca ccgtcgtggt
    13381 tcaagcggac accgacacgg ttgtcgctga tcacgttccc gcgtaccacc acccgggagg
    13441 agcccagcac cacgatgccg tccccccggt tccgctcgga tcggttgccc tcgatcaggt
    13501 tcccgtccga ccgctcgtcc atgacgatcc cgttgccgtc gttgtcgtgg acgtggttct
    13561 gggcgatcac gccatccgtg acgcccacgg agaggacgat gccgtggtca ccgttcccgt
    13621 agacctcgtt gccgcggatg tccagaccct tcgtgtagtc gtggggatcg accccgtagt
    13681 agacgttgtc gtggatgcgg ttgccctcca accgcagatc gcgcgctcgg aagctgtaga
    13741 ccccgaagaa gttgtcgtgg acatccgagt cggtcatcga gccggtcgcg ccgagacgcc
    13801 acgaggtccc gtaggccatg gagcggtccg atcccaggta cgcgatctcc gaaccgacga
    13861 tatccagcac cgaccccccc tcgtacagga tgaaggggcg gtcgccccgg tagtcgcggt
    13921 cgacctgctg ggcggacacg ttccacgagg tcaccagcac gtcctggaac gtggcccggg
    13981 cctggacccc gccgatgaac acgtccggac gggctgccag ccggatcgtg tccgtgccgt
    14041 ccccaccgaa ccgcaccgag gttcccggcc cctgggcgaa cgccgccttc agcagcagct
    14101 gcccgtcgcc cagatccttc acccatggcc ccggtcccac cgcggccgcc accgcgctga
    14161 agctgacggt cgaccgcgca tcgaagggga ccgagcgcag caccgtggct ccgcgcatca
    14221 ggtcgatccg gtccgtggag aggcggaggg tccgccggtg gagcccggcc agcttgcccg
    14281 ggtcccggac cacgtcgagc ggcttcgggt tgtagggacg cacgttccgc tctggtagcg
    14341 agcccgaccg agagccaccc gtcttccccg gagccgggct cggcgaggag gcggacgagc
    14401 tcaccgagga tgagggttca ccggccgatt gcgccgatgc ggtcggcgag gacagggaga
    14461 gcgccagcag ggccacgatc ggcagcgagg ccccgacacg gaggcgccgg ctcacgacgg
    14521 cccccggacc cgcatggcgg catcgttgtc ccgcaaggtg ttctcggcca cgaaccgctc
    14581 cacctgtgcc ttggaggatc cggagaacgc ggccttcccg ctctcggtga tccggttgcc
    14641 ccggatcacc ggggcgggcg tgaggtccgt gacatgcacg ccgtcgtcgg tgctcccgcc
    14701 gatccggttc ccggtgatgg tggcctcctc catacctccg gacacccaca cacccaccgt
    14761 gttgtcgagg atccggttgt ctccctggac ccggaccgtg ccatcatcca ggtacaaacc
    14821 ggaccgccgg ttgaagtcca cgaggttgcc ggtgagggtg accgacgagg ctccgctgga
    14881 caggaccaca ccatccgagc cgttgcccgc cacccggttc ccctccacgg cgatgtcgga
    14941 ggaccggtcg tggatcacga tcccgttccg gttctcgtag acggcgttcc cacggacccg
    15001 aacgtccttg gagccactca cgtcgatccc cgagccaccg ttcccgtgcg cgtcgttgcc
    15061 ctccagcgtg gcgtgatccg acccctggaa gaccacgaag ccatgctggg cgttgcccac
    15121 ggccaggttg tccttcacca cggcgttggt gcagtccacc gcgaggatga acccgtgctt
    15181 cccgttgtgc aggaaccgat cgccctggat caggaagcca tcgctgccgg tgtgcaggtc
    15241 cagcccgtag gacagcgagt ccttgatcac ggaatccgtg atccgcatgg gctccgcccc
    15301 gaaggtgtag aggccgtagt agttgccggc gaaggtggag ccgtcgacgg agccacccgt
    15361 gccggcggtc cgccacgaca cgccgtaccg ctcgtaggcg tcatacccga gcatgcgcat
    15421 cgtcgagtcc cgcacgatca tcgaggagcc gtcgcgtgcc aggacccagg ctcgcccgtc
    15481 gcggacatcc ccatcggccg tgccccggga cggatcccag gaggtgacca tggagtccac
    15541 gatcgtgagg tcgccccccc gggcctccag acccacctcc tggtccggtg tggagttgag
    15601 gcgcagggtg gcacccgaca gctcgaggga cgccccggtg tcgatcacca acgtgcctgt
    15661 cagcagccag gcctcgccct gccggcggag gatcgacccg tccgctccac gctgcagcgc
    15721 catctgcagc cccggcagat cggtctggcc cctcgtgagg tggaccaccc ctgtggcctg
    15781 atccacctca aggcgcaccg gcgtctggac ccggtgtctg gccagcaacg accacgccac
    15841 cgccgccagc ccacccgcca ggaccagggc caggagggtc cgccagacga ccctcatcgc
    15901 ttccccttcc acgcgaacag gtcgggatgc tcccgtggcg gcgggagtgg aggcagcgcc
    15961 gccggtgccc gtggaggcgg gggcgggggc agcgccgccg ccaccgatgt cggcgaggga
    16021 acggaaccgt tccgaccgct tcgttcgacc tcaccggtcc gggctgagac ctccacgtca
    16081 cgggtgagcc agcgctggcg acggatcgtg aagagagccg agatcttcac cagcgccatc
    16141 acgaacgatg tcacgatgaa cagcggcatc atcgtcaccg cgtgcctggg ccggcgctcc
    16201 aggtgcggaa gcatcttcgc cgagcggctg aggagccacc acgcgccgag gagcccggcc
    16261 accagctcgt tcccggcgat cagcgccagc accatgatcg tgggtgagac cagcagcgtg
    16321 aacgagctca ccgccttgtc gatcatggag aacgcgagga acggatggtg ccatacccag
    16381 ccgtccttca gggctcgcag gtcgctcctc cacgtgttcc gggaccagcg cagccgctgc
    16441 ttcacgaacc cgagagcatc cggggggaag gaggaccaca cccgtgccga acgctggagc
    16501 acggtgaggt gcccaccttc gaggatcagc gtggtcaggc gcttgtcgtc gcccgacatg
    16561 cagcgcaccc ccaggaacgt ctcgttcatg aaccggtcgg cgtggcggag caggagctcg
    16621 cgccggtaga cggccgtccg accggagatg caggagatgg ccctgccccg cactgtctgg
    16681 gccgcgacct cgtcgaagta ccggtagtcc aggtacatgt cgttcaggtg ctgccagggt
    16741 gtccgggggt tcgcgacgtt ctgccgggtg gccaccccgc ccaccttcgg atcggcgaag
    16801 ggcatgcaca cgagctgggc cacgtcggac gcccagatcg tgtccgagtc caccagcgcc
    16861 accagcggcg tggaggtcgc ctcccaaccg gtcttcagcg ccggtcgctt gcccgggacc
    16921 ggcgtgacga tgacccgcac cgggtactcc gacgcgatgt cgatgcaggt ccggtcggtg
    16981 tgatcgatca ccaggatgat ctcctccacg tcgttgcgca gccacgactc gagcgcgtag
    17041 cggaacgtct gcggatcctc ctggtacacc ggcaccacga tcgagagcgg ttcgcggtgg
    17101 tcgttctcca ccgagcggta caggacggcg ggcaagcgtc ggatcagcca gctcgtccac
    17161 cggatcaccc cgaggatccc gagcgggacg aggaacgcga ggctcccgat ccagtccatc
    17221 agctggcgcg cctgaccgcc tggccgtcgg ccgagggatc ggcatcgggc tcgatcgtcc
    17281 ccgccacggg cggtgccgag ggcacggtgg tgggtccgtc ggccgcctcg tgccggtcgg
    17341 gcgtgatccc ctggccgtcg gccgccacct gctcgttcac ctcgtccacg gcctggagga
    17401 gcggcatctc cacgcccaca tgccgggcga agccgaggaa ccccttcgtg tccttgggca
    17461 ggcacgcacc accgtacggc gctcccgcgt tgatcccgta ctcggggttg tacgagccct
    17521 cggccgaccg ggagacgatg cgcgccacat cggccacgtc catgcccagc cggttgccca
    17581 cccgccagat ctcgttccag aagctgatct tggtggcgtt gaacaggttg tgggcgcact
    17641 tcaccagctc ggccacgcga gggtcaccga acgtgtggat ctccccgccg aacggctcga
    17701 acagccggcg catccgctcc agcgtccgct tgctccggga ggccaccacc gtcatccacg
    17761 ggttcaggaa gtcctccagg gcgcaggcgg cgcgcaggaa ctcggggttc gccgccaggg
    17821 agaaccgctc gccctggcgg ccgcccgtgg cctcctccag gatcggccgg accaggccgt
    17881 cggtggtccc gggaggaacc gtcgagcgga cgaccacggt gtggagcgcg tggctgtcct
    17941 tcatggcctc cgccacctgc tccgcgcccc gtcggaaggg accgaggtcc cagcgatggc
    18001 cgtcgttcgg ggtgggcaag gtcaggaaca cgatgctggg ctcgctgggg agacggacat
    18061 ggtcggtcgc gccgaagccc tcggagcgca gcgcgtcgat ccgcctcgga tcgatgtcga
    18121 cgaacgacac cctatgtccg tgccgggcga accccttgcc ggtggcggtc cccaccacgc
    18181 ccgaccccac gatcaccacg tgtgccatct tggatctccc ctctctctgc tcccgccccg
    18241 ggccgtacgg cccggggccc tctcatccgt cgtcggtcat ccggactccg tcgtccgcgc
    18301 tcatcggtgg atcctgcagc agggccggac cggcctcccg gctcggcgcc gtcacggggc
    18361 cggcgaccac cgcgccgtcg gccaggaccg agcccggatc gagcctgatc ccgctccgca
    18421 cccgggcgcc cctcccgagg atcgcctggc tgacgctggc cgatccctcg accgctgcct
    18481 cgtcgaagag caccgcatcg ccgatcctgg ctcccggctc gatcgcgcac ccggctccca
    18541 ccacgaccgg gccggcgagg ctcgcggccc gatccacggc ggcgtcccgg tgcactgccg
    18601 ccaggggccc gatccgccgc agcccggggg cccacggcca ggcacagcga ccctcgagca
    18661 ccgcgcggtg cccggcccgg taccgctccg gcgtcccgag gtcctgccag taggcgtgcg
    18721 ggatcacgcc cgtcaccagg gcaccctcgt ccacgagggc ggggaagacg tcgcgctcga
    18781 acgacaggga gccatcgccc gggagcaggt cgaaggccga tgcatccagc acgtacgccc
    18841 ccgcgttcac cgtccggtga gcggtggtac ccggggccgg cttctccagg aaccgccgga
    18901 cccggccagc gtcgtcgcgg accacgaccc cgtagctcga cgggtcctca acgcgcatca
    18961 gggccagcgt ggcgacggac cgggagcggc ggtgcgcttc gatcagccgc tcgtaccgga
    19021 cgtcggacaa gatgtcgccg ttcaggacca ccaccgagcg ctcgccggtc ccggccagga
    19081 gccgtcgcgc ggcgccggcc gtatccagcg gacgctcctc ggtcaccagg tccacccgga
    19141 tccccgcggc cctgccgagg tcctggatcg gcgcgaacgg cccggcatct gggcccacca
    19201 ggaacgttgc ccgcccacac ccggcctggg ccagcctgcg gagcagcccc gccgcgtacg
    19261 gcgcgcccat gaagggcacc agcggcttcg gacgtgactc cgtcagcggg cggaggcggg
    19321 ttcccttccc accggccagc accacggctc gccagggggc gtggcggagc ggatccgggt
    19381 tcgtggggag catgcgggcc gtccttggtt gacgttcgtg ttacgaagtt gtcctgaggg
    19441 tttcccgggg gttccccgcc cgaaacacgg agcgcggtga acgtgggtta ggagatcgtt
    19501 aaggatcccg ccgacccggg cccggagcgc gttatccccg cccaccagac cgggtatcga
    19561 tcaggcatgg cgcgcgcgat ctggagcgga gcggtctcgt tcgggctggt caacgtgccg
    19621 gtccgccttt actcggccac cgagtccaag gacgtccggt tccacgagtt ccagcaggga
    19681 accggacgtc ggatccgcca cgagcgggtg gccgagggca ccgaccagga ggtcccctac
    19741 gaggacgtgg tcaagggcta cgagtacgcg gacgggcagt acgtcaccct gacccaggag
    19801 gagttggagg ccgcccagcc cgagcgcacc cggggcatcg cgatcgagga cttcgtggaa
    19861 cttgcggacg tcgaccccat cttcttccag aagacctact acctggatcc gggccccggg
    19921 gccgagaagc cgtacgcgct cctgctgcgc gcgatggagg agaccggccg ggtgggcatc
    19981 gcccggttcg tgttccggac caaggagcac ctggccgcga tccggcccat ggacggactg
    20041 ctcgtcctgg agacgatgtt cttcgcggac gaggtccgcg acgtgggcga catcgacggc
    20101 gttccggcgc aggccgatct ggaccaacgg gaggtcgaca tggc
//
LOCUS       DASQQQ010000143         3351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_5500, whole genome shotgun sequence.
ACCESSION   DASQQQ010000143 DASQQQ010000000
VERSION     DASQQQ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5500"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..806)
                     /locus_tag="VE646_05930"
     CDS             complement(15..806)
                     /locus_tag="VE646_05930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZD79560.1"
                     /translation="MRIGLHVGHWERRPADAVDLVREAERMGVDSVWTSEAWGSDAVT
                     TISWLAARTSTIGVGTAVMQIPARTPAATAMTAITLDHLSGGRFRLGLGVSGPQVVEG
                     WHGVGSDDPLGRTREYVAIVRAALARNAPLEHHGRHYDLPLREGTGMGRALKVGVVPL
                     RADLPVYLAAMGPGNVALAAEIADGWLPILFSPERADLFRPWLEEGFARRGGPPERFD
                     VAPMAWAAVGEDLGSCRDRLRPAIALYVGGMGARDRNCYRRMVVR"
     gene            910..2352
                     /locus_tag="VE646_05935"
     CDS             910..2352
                     /locus_tag="VE646_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HZD79561.1"
                     /translation="MLEQAEQAVEAARRAGADYADARFVRDDSESITVRNQEMEGIDR
                     STSKGLGIRVLVGGYWGFAATARTEEADVRRTAELAVSIARAAARLPRDPVSLTQVEP
                     ATGTWHSPMQEDPFNVPLEEKIALLMEASRRMQEVRGLSFGEAGIDLFRRRTWFASSE
                     GAALEQTTVHSGGGIEATAVGEGDLQRRSFPNSFRGHIAAAGWEHVKRLALVQEAERT
                     ATEAVELLSAPECPSELTTLILDGDQVQLQVHESIGHPVELDRVLGMEEAYAGSSFLR
                     PEDRGRLRYGSELVSITADATLPGGLGTFGWDDEGVPAQRVPIIVDGIFQDFLSSRET
                     AGVVGQTSSGAMRADGWQHLPLIRMTNISIEPREGTLADIIGDTKDGIYAATNSSWSI
                     DDKRVNFQFGCEIAWRIKDGKLTEMFRNPNYTGITTEFWGSCDAVGGREDWVLWGTPN
                     CGKGQPGQVARVGHGASPARFRNVRVGVRG"
     gene            2352..>3351
                     /locus_tag="VE646_05940"
     CDS             2352..>3351
                     /locus_tag="VE646_05940"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HZD79562.1"
                     /translation="MPGLVGPDEVRRVAEAALELPGVDGVEVLFMHEWSGLTRFASSA
                     IHQSTWQEDTAIRVRVVDGGRIGVAATNDLSPEGARAAAASAREMALVVAPDPLFPGL
                     APPAAVPERDGFEEATASSTPEARAGSVAALVGQCPPGFAAAGAFETAAAEVAVANTE
                     GQFCWAPSTQASITTVVTGGEGGNGFAEVFAPSVEAIDPEALGRRAADKAMRSQDPRD
                     LEPGRYPVILEASAVSTLVGFLAWIGFGGRALLEGRSCFSGKEGQRVAAPTVTIMDDA
                     LSPLTVGMPFDFEGVPRRSVPLIEGGVFRDGVYDLRTAKQGGKQTTGHALPPPNPEG"
BASE COUNT          480 a         1213 c         1207 g          451 t
ORIGIN      
        1 tcctgctcgt agccctatcg caccaccatc cgccggtagc agttccgatc ccgggcgccc
       61 atccctccca cgtacagggc gatggcggga cggagccggt cccggcacga cccgaggtcc
      121 tcgcccaccg cggcccaggc catcggcgcg acgtcgaacc tctcaggcgg accccctcgg
      181 cgagcgaacc cttcctccag ccacggccgg aagaggtcgg cacgctcggg cgagaacagg
      241 atggggagcc agccgtcggc gatctcggcc gccagggcca cgttgccggg tcccatcgcc
      301 gccaggtaca cggggaggtc ggcccggagc ggcacgaccc cgaccttcag ggctcgtccc
      361 atcccggtcc cctcgcgcag cggaagatcg tagtgacgcc cgtggtgctc gaggggcgcg
      421 ttccgggcca gagcggctcg aacgatcgcc acgtactcgc gggtccggcc cagtgggtca
      481 tcggatccca ccccgtgcca gccttcgacc acctggggtc cggagacgcc gagtcccaac
      541 cggaaccgcc cgcccgacag atgatcgagc gtgatggccg tcatggcggt cgcggcgggg
      601 gtcctggccg ggatctgcat gaccgcggtc cccaccccga tcgtcgacgt ccgagcggcc
      661 agccagctga tcgtggtgac ggcgtccgag ccccaggcct cggaggtcca gaccgagtcc
      721 acgcccatcc gttccgcctc gcgcaccagg tccacggcgt cggcgggacg ccgttcccag
      781 tgaccgacgt gcaggccgat cctcacggct gcagcctagc cgccccagca tgccgcgccg
      841 gggctgctcc ggtaggcttg cggcccgtgg ctcctcgcgg ggccgtccgg tcggatcgga
      901 ggcgacgcct tgctcgagca agcggaacag gcggtcgagg ccgcacgcag ggcgggagcc
      961 gactacgccg atgctcgctt cgtccgggat gactccgagt ccatcaccgt gcgcaaccag
     1021 gagatggagg gcatcgaccg ctccacctcc aagggcctgg ggatccgggt gctggttggc
     1081 gggtattggg ggttcgcggc aaccgcccgc accgaggagg ccgacgttcg gcgcaccgcg
     1141 gaactagccg tgtcgatcgc tcgcgccgcg gcccgactgc cccgtgaccc cgtgtcgctc
     1201 acccaggtcg aaccggccac gggcacctgg cacagcccga tgcaggagga ccccttcaac
     1261 gtccctctcg aggagaagat cgccctgctc atggaggcca gccggcggat gcaagaggtc
     1321 cgggggctga gcttcgggga ggccgggatc gacctgttcc gccggcgcac ctggttcgcc
     1381 tcctcggagg gagccgcgct cgagcagacc acggtccact ccggcggtgg gatcgaggcc
     1441 accgccgtgg gtgagggcga tctgcagcgt cgctccttcc ccaactcctt ccgagggcac
     1501 atcgccgcag ccgggtggga gcacgtgaag cgcctggcgc tggtccagga ggccgagcgc
     1561 accgccaccg aggcggtgga gctgctctcc gcccccgagt gcccgagcga gctcaccacc
     1621 ctgatcctgg acggcgatca ggtgcagctc caggtccacg agtcgatcgg tcaccccgta
     1681 gagctcgacc gcgtgctggg catggaggag gcctacgcgg gctcctcgtt cctgcgcccg
     1741 gaggaccggg gccggctccg ctacggcagc gagctggtct ccatcaccgc cgacgccacc
     1801 ctgcccggcg gtctgggcac cttcggttgg gacgacgagg gcgtgccggc gcagcgggtg
     1861 ccgatcatcg tggacggcat cttccaggac ttcctctcca gcagggaaac ggccggcgtg
     1921 gtgggacaga cctcgtccgg cgccatgcgc gccgacggct ggcagcacct gccgctgatc
     1981 cgcatgacca acatcagcat cgagccccgc gagggcaccc tggcggacat catcggggac
     2041 acgaaggacg ggatctacgc ggccacgaac agctcatggt cgatcgacga caagcgagtg
     2101 aacttccagt tcggctgcga gatcgcctgg cgcatcaagg acggcaagct gaccgagatg
     2161 ttccgcaacc ccaactacac gggcatcacc accgagttct gggggtcctg cgacgcggtg
     2221 ggcggccggg aggactgggt cctgtggggc accccgaact gcgggaaggg acagcccggc
     2281 caggtggcca gggtggggca cggggcctcg ccggcccggt tccgcaacgt ccgggtgggg
     2341 gtccggggct gatgcccggc ctggtcggcc ccgacgaggt ccgccgcgtg gccgaagccg
     2401 ccctggagct gcccggcgtg gacggcgtcg aggtgctgtt catgcacgag tggagcgggc
     2461 tcacccgctt cgcgtcgtcg gccatccacc agagcacgtg gcaggaggac acggccatcc
     2521 gggtgcgggt ggtggatggc ggccggatcg gcgtggccgc caccaacgac ctgtccccgg
     2581 agggagcacg ggcggccgca gcgagcgccc gggagatggc cctcgtcgtc gcccccgacc
     2641 cgctgttccc ggggctggct cctccggcgg cggtcccgga gcgggacggc ttcgaggagg
     2701 cgaccgcgtc ctccacaccc gaggcccggg ccgggtccgt ggccgcgctc gtggggcagt
     2761 gcccgccggg gttcgcggcc gccggcgcgt tcgagaccgc cgctgccgag gtggcggtgg
     2821 ccaacaccga gggccagttc tgctgggctc catccacgca ggcctcgatc accacggtgg
     2881 tcaccggcgg cgagggcggg aacgggttcg ccgaggtctt cgcaccctcg gtggaggcga
     2941 tcgatcccga ggccctcgga cgccgggcgg cggacaaggc gatgcgctcc caggaccctc
     3001 gcgatctgga gcccggccgg taccccgtga tcctggaggc ctccgccgtc tccacgctcg
     3061 tggggttcct ggcgtggatc ggcttcggag gtcgagccct gctggaggga cggtcctgct
     3121 tctcgggcaa ggaggggcag cgcgtcgcgg cgccgaccgt cacgatcatg gacgatgccc
     3181 tctcgccgct cacagtgggg atgcccttcg acttcgaggg ggtgccccgc cgatcggtgc
     3241 ccctgatcga gggcggggtc ttccgcgacg gcgtctacga cctgcgcacc gccaagcagg
     3301 gggggaagca gaccaccggc catgccctgc ctccccccaa ccccgagggc c
//
LOCUS       DASQQQ010000144         5033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_394842, whole genome shotgun sequence.
ACCESSION   DASQQQ010000144 DASQQQ010000000
VERSION     DASQQQ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394842"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VE646_05945"
     CDS             complement(<1..1041)
                     /locus_tag="VE646_05945"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZD79563.1"
                     /translation="MKEPKFIFVTGGVASGLGKGITTASLGRLFKARSLRVALQKLDP
                     YVNVDPGTMNPFEHGEVFVLDDGAETDLDLGHYERFTDENLHRGSNLTTGAVYSAVIQ
                     KERRGDYLGKTVQVIPHITDEIKERIRSYAEAENADLLIVEVGGTVGDIESLPFLEAI
                     RQLRNEVGRDRCAFVHVSLMPFIGPSGELKTKPTQHSVKELRSLGLQPDAIVCRSDRP
                     IGRHLKEKISLLCDVPISGVVSAPDADSIYRVPLILHQEGLDQVLAHHLRIEQAPDLA
                     EWKGLVERIDVSTDPVQIAIVGKYVNLRDAYLSVIEALRHGGFYHRARVDITWVASDD
                     LAKGDTSVVLAGI"
     gene            complement(1124..1870)
                     /locus_tag="VE646_05950"
     CDS             complement(1124..1870)
                     /locus_tag="VE646_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79564.1"
                     /translation="MRWGRLTVVNYRGVGIPRALGLLIGAAAVLWCLAYALARSVPSS
                     AWASLIGCLLVLVAGLVDDVVAVGPRGLRNHLRALASGHVTTGIVKVIVIVGSAVVVI
                     ASTRPGHWWVGAAGVVLVAASANVWNGLDLRPGRALKAYLPMALPFLLAGDLAAAPAL
                     VGVSLGALVGLPLDLRERAMLGDGGANLLGFSVGVGLVLVLPGWGVAAAAVAAVALNV
                     LADTVSFSRVIGELGPLRWADRLGRLPAPD"
     gene            complement(1867..2532)
                     /locus_tag="VE646_05955"
     CDS             complement(1867..2532)
                     /locus_tag="VE646_05955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZD79565.1"
                     /translation="MTALVAARNEEDRVAATVAALRSVPEVTEVVVVDDGSRDGTAGA
                     ARLAGARVLRSSRHRGKGGALRSALIALPTPDVWLLADADLGVTAAGVRPALTCVLEG
                     RADLAVGSLPPQPGGGFGLVKRAAARAIRALSGFAAEEPLSGQRAVTSEGMRVCGPLA
                     AGFGVETAMTIDAVRAGLRVLEVPIEVRHRPTGRTLSGFLHRGRQGLDIARAVAARAV
                     GRR"
     gene            complement(2544..3485)
                     /locus_tag="VE646_05960"
     CDS             complement(2544..3485)
                     /locus_tag="VE646_05960"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HZD79566.1"
                     /translation="MISFRYHLITIVAIFLALGLGLLAGTTVVDQSLVSNLRNTTEIL
                     RSDLRDQRAEAARYQAQVGAAQEASNILPVLDPGLLAGVPVVLVAGPELDGAVLSITR
                     DALDEARANLLGVLSLTDRMAAIDETSRRALAKILGAPADTTPEVLQRMTAQTLSQRL
                     TSGLPRRGAQPAQDDVLDQLLTENFLTSPGITKDDVQAIGGRDQVVVVLAGGPTVPVV
                     SPDRFLVPLVGDLVARGTSVAAGESTTTSYPFVETLRADGSPADGTRMVTVDDLDLSI
                     GGAALVLGLEHLMFAGQGGNYGVKGSPTDVRPIPPLT"
     gene            complement(3482..4744)
                     /gene="steA"
                     /locus_tag="VE646_05965"
     CDS             complement(3482..4744)
                     /gene="steA"
                     /locus_tag="VE646_05965"
                     /inference="COORDINATES: protein motif:HMM:NF040608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HZD79567.1"
                     /translation="MLEGPTTGGAEAMATTGRSRGLLRRPQRGPRPHEAVARVDQRTK
                     RLVRRAKQGEIAIIDHEDLDRVAAEGLVEAGVSGVVNAAPSISGRYPNLGPLILLDAG
                     VPLVDGVGPLLLRKVRDGDVVRVDGDRVFLDDRLVGVGVRQSERSVRAAMEEARAGLA
                     EQFESFARNTVDFMQRERDLLFGGAGLPEIDLDMSGRPVLVVVRGYSYKEDLAVLRPY
                     IRDVRPVLVGVDGGADAVLEAGYRPDLIIGDMDSVSDEALLMAVPRGGARRAHRATRI
                     VVHAYRDGFAPGGERLDQLGVPYQVVRAAGTSEDVAFLLSHEKGASLIVAVGSHGNLR
                     EFLDKGRLGMASTFLVRLRVGEILMDAKGVSRLYSPRVRTRDALLLLGAALFAMVMVV
                     VISPALRLYVIQLFEQFRQWLFHLRELL"
     gene            complement(4801..>5033)
                     /locus_tag="VE646_05970"
     CDS             complement(4801..>5033)
                     /locus_tag="VE646_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014693301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HZD79568.1"
                     /translation="DRHLVVSKTGGTASVAVVDGTDRIGELSRMLSGLPESQAAAVHA
                     EELLTQADRVKRGRGRRRPARAGAASAHRTAR"
BASE COUNT          785 a         1904 c         1678 g          666 t
ORIGIN      
        1 gatcccggcc aggacgaccg aggtgtcgcc cttggccagg tcgtcggagg ccacccaggt
       61 gatgtcgacc ctggcacggt ggtagaagcc gccgtgccgt agggcctcga tcaccgacag
      121 gtaggcatcg cggaggttca cgtacttgcc cacgatcgcg atctggaccg ggtcggtgga
      181 cacgtcgatc cgctcgacca ggcccttcca ctcggccagg tcgggggcct gctcgatccg
      241 cagatgatgc gccaggacct ggtccagccc ctcctggtgc aggatcaggg gcacgcggta
      301 gatcgaatcg gcgtcgggtg cggacacgac gcccgagatc ggcacgtcgc acagcagcga
      361 gatcttctcc ttcaggtggc gcccgatggg ccggtcggat cggcaaacga tcgcatcggg
      421 ctgcagaccg agcgatcgca gctccttgac cgagtgctgg gtgggcttgg tcttgagctc
      481 cccgctgggg ccgatgaagg gcatcaggga cacgtggacg aacgcgcacc gatcgcgccc
      541 cacctcgttc cgtagctggc ggatggcctc taggaagggc agggactcga tatcgcccac
      601 cgtgcccccg acctcgacga tcagcaggtc ggcgttctcc gcctctgcgt atgagcggat
      661 ccgctccttg atctcgtccg tgatgtgcgg gatcacctgg acggtcttgc ccaggtagtc
      721 gccgcggcgc tccttctgga tcacggccga gtagacggcg ccggtggtca ggttggagcc
      781 gcggtgcagg ttctcgtccg tgaaccgctc gtagtgaccc aggtccagat cggtctcggc
      841 accgtcgtcg agcacgaaca cctctccgtg ctcgaagggg ttcatggtgc ccggatcgac
      901 gttgacgtag gggtccagct tctggagggc gacccgcaga ctgcgggcct tgaacaggcg
      961 ccccaaagaa gcggtggtga tccccttgcc gagtcccgag gcgactccgc cggtcacgaa
     1021 gatgaacttc ggctctttca acacctcggg attgcatgct agcacaggcc gaggacggga
     1081 ccggcagatc cgagcgtttc cgctccccgg tcgaccccaa gggtcaatcc ggtgcgggca
     1141 gccgccccag gcggtccgcc catcggagcg gtccgagctc cccgatcacg cgggagaagc
     1201 tcaccgtatc ggccaggacg ttcaacgcca ccgctgcgac ggcggccgcg gcgacccccc
     1261 atccggggag cacgaggacg agcccgaccc ccacggagaa gcccagcagg ttcgcgccgc
     1321 cgtcgcccag catggcgcgc tcgcgaaggt cgaggggcag gccgaccagg gcccccaggg
     1381 acacgccgac cagcgccggt gcggcggcca gatcgcccgc cagcaggaac ggcagggcca
     1441 tgggcaggta tgccttgagc gcacgacccg ggcggaggtc gagcccgttc cagacgttgg
     1501 cggaggcggc caccaggacc actcccgccg cccccaccca ccagtgaccg ggtcgggtgg
     1561 aggcgatcac caccacggcc gagcccacga tcacgatcac cttcacgatg cccgtcgtca
     1621 cgtgaccgga cgcaagggcc cgcaggtggt tgcggagtcc gcgcggtccc acggccacca
     1681 catcgtccac cagtccggcg acgaggacca gtaggcaccc gatcaggctg gcccacgcgc
     1741 tggaggggac cgatcgggcg agggcgtacg ccaggcacca gagcaccgcc gctgctccga
     1801 tcagcaggcc caaggcccga gggatgccca ccccccggta gttgacgacg gtgagtcggc
     1861 cccacctcat cgacgtccca ccgcccgcgc cgccaccgcg cgcgcgatgt ccaggccctg
     1921 acggccgcga tgcaggaacc ccgacagggt ccggccggtc ggccggtgcc gcacctcgat
     1981 ggggacctcg agcacccgca gcccggcccg caccgcgtcg atcgtcatcg ccgtctccac
     2041 gccaaagccg gccgccagtg ggccacagac ccgcatccct tccgaggtca ccgctcgctg
     2101 accggacaat ggctcctcgg ccgcgaagcc gctcagcgct cggatcgcgc gggccgccgc
     2161 gcgcttgacc aggccgaacc caccgccggg ctggggaggc aaggaaccca cggcgaggtc
     2221 ggcccggcct tccagcacgc acgtcagcgc cggtcgaacc cctgccgccg tgacccccag
     2281 gtcggcatcg gccaggagcc acacgtcggg ggtcggcagc gcgatgaggg ccgaccgcaa
     2341 cgccccgccc ttgccccggt gcctggagct tcggagcacc cgcgctccgg ccagccgagc
     2401 ggctccggcg gtgccgtccc gcgagccgtc gtccaccacc accacctcgg tgacctcggg
     2461 gaccgatcgc agcgcggcaa ccgtggcggc cacccgatcc tcctcgttcc gggcggcgac
     2521 caacgcggtc accaccggcg gcctcacgtg agcgggggga tcggacgcac gtccgtgggg
     2581 ctgcccttca cgccgtagtt gccgccctgc ccggcgaaca tcagatgctc caaccccagg
     2641 accaacgcgg ctcccccgat cgacaggtcc aggtcgtcca cggtgaccat gcgggtgccg
     2701 tccgcagggg aaccgtcggc acgcagcgtc tccacgaacg gataggaggt cgtggtgctc
     2761 tccccggccg cgacgctcgt cccacgcgcc accagatcgc ccaccagcgg caccaggaac
     2821 cgatccgggc tcaccaccgg aacggtgggc cctcccgcca gcaccacgac gacctggtcg
     2881 cgccccccga tggcctggac gtcgtccttg gtgatgccgg gcgaggtgag gaagttctcg
     2941 gtcagcagct ggtcgaggac gtcgtcctgc gcgggctgcg ccccgcgccg ggggagcccg
     3001 ctggtcagac gctgtgagag cgtctgcgcc gtcatccgct ggaggacctc gggggtggta
     3061 tcggccggtg ctccgaggat cttggccagc gctcgacgcg acgtctcgtc gatcgccgcc
     3121 atccgatccg tcaagctcag caccccgagg aggttggctc gagcctcgtc caaggcgtca
     3181 cgcgtgatgg acagcaccgc cccatcgagc tccggacccg ccaccaggac gacggggacc
     3241 ccggccagca acccgggatc gagcaccggc aggatgttgc tcgcctcctg cgccgccccc
     3301 acctgcgcct ggtacctcgc ggcctccgcc cgctggtccc ggaggtccga gcgcaggatc
     3361 tcggtcgtgt tgcgcaggtt gctgaccagg ctctgatcca ccacggtggt ccccgcgagc
     3421 aggcccagcc ccagggccag gaagatggcc acgatggtga tgaggtggta ccggaacgag
     3481 atcacaggag ctccctgagg tggaacagcc actgacggaa ctgctcgaac agttggatca
     3541 cgtacagccg gagcgccggc gagatcacga cgaccatgac catcgcgaac aacgcggccc
     3601 ccaggagcag gagcgcgtcc cgggtgcgga cccgggggct gtacaggcgc gacacgccct
     3661 tcgcgtccat caggatctcc cccaccctca gccggacgag gaaggtgctg gccatgccca
     3721 gccggccctt gtccaggaac tcgcgtaggt tcccgtggct ccccaccgcc acgatcaggc
     3781 tggctccctt ctcatgggac agcaggaacg cgacgtcttc gctggtgccg gccgcccgca
     3841 ccacctggta cgggacgccg agctggtcca ggcgctcgcc gccgggggcg aagccgtcgc
     3901 ggtaggcgtg caccacgatc cgcgtggccc ggtgcgcgcg ccgggcgccc ccgcgcggca
     3961 ccgccatcag gagcgcctca tcggagacgc tgtccatgtc cccgatgatc aggtcggggc
     4021 ggtagcccgc ctccaacacg gcgtccgcgc cgccgtccac ccccaccagg accggacgga
     4081 catcgcggat gtacggccgc agcaccgcca ggtcctcctt gtaggagtag ccgcggacga
     4141 cgaccagcac cggacgcccc gacatgtcga ggtcgatctc gggcagcccg gcccccccga
     4201 agagcagatc gcgctcccgc tgcatgaagt cgacggtgtt gcgggcgaag ctctcgaact
     4261 gctcggcgag tccggcgcgc gcctcctcca tcgcggcgcg gaccgagcgc tccgactggc
     4321 gaacccccac gcccaccagc cggtcgtcca ggaacacccg gtcgccgtcc acccggacca
     4381 cgtccccgtc ccgcaccttc cgcagcagca ggggaccgac cccgtccacc agcgggaccc
     4441 cggcgtccag caggatcagc gggccaaggt tcggatatcg gccgctgatc gacggtgccg
     4501 cgttcaccac cccgctgacc cccgcctcca ccagcccctc cgcggccacc cggtccaggt
     4561 cctcgtgatc gatgatggcg atctctccct gcttcgcccg ccgtaccagg cgcttggtcc
     4621 gctgatccac ccgggccacc gcttcatggg gtcgaggccc tcgctgcggc ctccgcagca
     4681 gacccctgga cctgcctgtc gtcgccatcg cctccgcgcc cccggtcgtc ggcccttcga
     4741 gcatgcacgc cgctcgaggg gcaccgaaca gggtagccga acgggaccca ggtccccagg
     4801 tcagcgggcg gttcggtggg ccgaggcagc cccggcacgc gccggacggc ggcggccgcg
     4861 gccccgcttg acgcggtcgg cctgggtcag cagctcctcg gcgtgcaccg cggcggcctg
     4921 gctctcgggc agacccgaga gcatccggga cagctcgccg atgcgatcgg tcccgtcgac
     4981 caccgccacc gaggccgttc ctccggtctt gctgaccacc aggtggcgat cgg
//
LOCUS       DASQQQ010000145         4040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_283969, whole genome shotgun sequence.
ACCESSION   DASQQQ010000145 DASQQQ010000000
VERSION     DASQQQ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283969"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VE646_05975"
     CDS             complement(<1..437)
                     /locus_tag="VE646_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZD79569.1"
                     /translation="MGQAPIRVGEVEVAVVCEGFAPLPLEEELPGQDVDWAGERTIHP
                     WAFVDERTWAWHVHAFALFTRAGVVMVDVGLGDFPPYRPWAEQVDPEAALASAGVRPG
                     DVRAVIHTHLHADHAGGAVVAGRPRFPNAVHHVHPADWSAFGAP"
     gene            654..1109
                     /locus_tag="VE646_05980"
     CDS             654..1109
                     /locus_tag="VE646_05980"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HZD79570.1"
                     /translation="MERFLPKTKRIALVAHDNKKRDLLEWARYNATTLGEHHLFATGT
                     TGSMLESELGLSVEKLQSGPLGGDLQIGAQIADGAIDLLVFFWDPLEPQPHDPDVKAL
                     LRIAVVWNVPIACNRASADFIISSPLMTGRYERLPPNYEAYVMREIGWG"
     gene            1161..1793
                     /locus_tag="VE646_05985"
     CDS             1161..1793
                     /locus_tag="VE646_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003998968.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HZD79571.1"
                     /translation="MARTAGTAEPFVPDRPSLSNLRRAVQDCRGCELYRDATRAVFGE
                     GRRRARVMLVGEVPGDREDIEGRPFVGPAGRELDRGLDAAGIDREDAYVTNAVKHFRF
                     ERRGKRRIHRKPTLGQMSACRPWLDAEIAVVRPEVVVCLGATATRAVAGPGVTVSRNR
                     GTFVETDLEPLVTITVHPSAILRSPDEASRRAEREAFERDLSLVARAIRA"
     gene            1869..2525
                     /locus_tag="VE646_05990"
     CDS             1869..2525
                     /locus_tag="VE646_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79572.1"
                     /translation="MAKPSQREVVRALLERHGRTHAQDLGVDVSRNTPSPLFRLLIAS
                     LLFSARISSNVALEAARALAEKGWTTAPKMAGSTWSQRTKTLNRAGYARYDESTSRMI
                     GESTDLLLERWGGDLRKLRAEADRDPDQERPLLKAFKGIGDRGVDIFFREVQVAWDEI
                     RPFADRPALDAAERLGLPADPGKLEGLVSRKDFPRLVAALVRTGIEQDFDQIQDQAAA
                     "
     gene            2522..3562
                     /locus_tag="VE646_05995"
     CDS             2522..3562
                     /locus_tag="VE646_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZD79573.1"
                     /translation="MSLPEPVGRPAPSFGIRPPPPREIQLEVTGACNLRCTMCLVAYR
                     PTLSRSEGSMSFETFRAVVDDVPGLERITLQGLGEPLLAPDLFRMIAYAAERGIRMGF
                     NTNGTLLTRSRAVRLVRAGLGWLHISVDGATAETYESIRSGSDFDRVTRNVRGLVEAM
                     RERSASEPELSLVFVAMRRNVRQLPDMVRLAASWGVPRLWVQNLSHSFADVSPMTDYR
                     GIGEFTAAEALWTDRDGEVEDLFDRARRLADRLAIELRLPRLEERSTSRGSGTPGCDW
                     PWRSTFVNHDGTVQPCCMLMGADRGVLGSVRTERFGEVWDGAGYRAFRRRLLSDQPPD
                     VCRGCSMYRGVF"
     gene            complement(3565..>4040)
                     /locus_tag="VE646_06000"
     CDS             complement(3565..>4040)
                     /locus_tag="VE646_06000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZD79574.1"
                     /translation="VLTGSEAERDLVEAVRRTAGVPVHSLAGELSVPELAALIERASI
                     LLSNNTAPVHLAAGVGTPVVVLYALTNPQHTPWGVPSRVLIHDVPCRFCYRSVCPQGH
                     HRCLRGIEPVTVVDALHELWRSTRGELRGTDRPVAGRVVPIDTPPVRLPVPATLG"
BASE COUNT          580 a         1416 c         1476 g          568 t
ORIGIN      
        1 ggggcgccga acgccgacca gtcggccggg tggacgtggt gcacggcgtt ggggaaccgg
       61 ggccggccgg cgaccaccgc tccgcccgca tgatcggcgt gcaggtgggt gtggatcacc
      121 gcccgcacgt ccccgggcct caccccggcc gacgccaggg ccgcctctgg gtcgacctgc
      181 tcggcccacg gccggtacgg ggggaagtcg ccaaggccga cgtccaccat gaccacgccc
      241 gcccgcgtga acagcgcgaa cgcgtgcacg tgccacgccc acgttcgctc gtccacgaag
      301 gcccagggat ggatggtccg ttcgcccgcc cagtccacgt cctgcccggg gagctcctcc
      361 tccagtggga gtggggcgaa cccttcgcac accaccgcca cctcgacctc gcccacccgg
      421 atcggcgcct gtcccatccc ctcgcctccc tcgcgtccgt ctacccgccg ctggcggaga
      481 agtgctggtg gtccttcggg ccgtgccggc tccggtaccg gacccaggcg atggcccgga
      541 acgcccgcga tggcgatcgc acggacgcat gttccaccat ccggggtccc ggcgcgggtc
      601 gaggcggccc tgccttgacc gatgacgctt cgggccacag gatgaccgtc gccatggaac
      661 gcttcctgcc caagaccaaa cgcatcgccc tggtcgctca cgacaacaag aagcgcgatc
      721 tcctggagtg ggccaggtac aacgcgacca cccttggcga gcaccacctc ttcgccaccg
      781 gcacgacggg gtcgatgctc gagagcgagc tcgggctgtc cgtggagaag ctccagagcg
      841 gcccgctcgg cggcgacctg cagatcggcg ctcagatcgc cgacggggcg atcgacctcc
      901 tggtgttctt ctgggacccg ctggagccgc agccgcacga cccggacgtg aaggccctgc
      961 tccggatcgc cgtggtgtgg aacgtcccca tcgcctgcaa ccgtgcctcg gccgacttca
     1021 tcatctcctc accgctcatg accgggcggt acgagcggct gcccccgaac tacgaggcct
     1081 acgtgatgcg ggagatcggc tggggatagt ctgccctcgg cggtcggttg cccgtctccg
     1141 ggagcgggta cgagcccggc gtggcgcgaa ccgccgggac cgcggaaccc ttcgtccccg
     1201 accgaccgtc gctgtccaac ctccggcgag ccgtccagga ctgccggggc tgcgagctgt
     1261 accgggacgc caccagggcg gtgttcgggg agggcaggcg ccgggcccgg gtgatgctgg
     1321 tgggagaggt ccctggcgac cgggaggata tcgagggccg gcccttcgta ggtccggccg
     1381 gacgcgagct ggaccggggg ctcgacgcgg ccgggatcga tcgtgaggac gcgtacgtga
     1441 ccaacgccgt caagcacttc cggttcgagc gacggggaaa gcgccggatc caccgcaagc
     1501 ccacgctggg tcagatgagc gcctgccggc cctggctgga cgcggagatc gcggtggtgc
     1561 gcccggaggt ggtggtgtgc ttgggcgcca ccgccacccg ggcggttgcc ggacccggcg
     1621 tcaccgtctc ccggaaccgg ggcacgttcg tggagaccga tctggagccg ctggtgacca
     1681 tcacggtgca tccctcggcc atcctgcgct cgccggacga agcctcccgg cgggccgagc
     1741 gcgaggcgtt cgagcgcgac ctgtccctgg tggcccgcgc gatccgggcc tgaaccgctc
     1801 gcttcggagc cccgtttgga ccgcacgagg tgtgggcatc cacccctgga ggacggaagg
     1861 agacgcccat ggccaagccc tcccaacgcg aggtcgttcg ggcgctgctc gagcgtcacg
     1921 ggcggaccca cgcccaggac ctgggggtcg acgtgtcccg caacacgccg tccccgctct
     1981 tccggctgct gatcgcatca ctgctcttct cggcgaggat cagctcgaac gttgcgctgg
     2041 aggcggcccg agcgctggcc gagaaggggt ggaccacggc tccgaagatg gccggatcca
     2101 cctggtcgca gcggaccaag acgttgaacc gggccgggta cgcccgctac gacgagagca
     2161 cgtcccggat gatcggcgag agcacggacc tgctgctgga gcgatggggt ggcgacctgc
     2221 gcaagctccg ggcagaggcg gaccgcgacc cggaccagga gcggcccctg ctgaaggcgt
     2281 tcaaggggat cggcgatcgc ggggtggaca tcttcttccg cgaggtgcag gtggcctggg
     2341 acgagatccg tccgttcgcc gaccggcccg ccctggatgc cgccgagcgc ctcgggctgc
     2401 cggccgaccc gggcaagctg gaggggctgg tctcgaggaa ggacttccct cgactggtgg
     2461 ccgcgctggt gcgcacgggg atcgagcagg acttcgacca gatccaggac caggcggccg
     2521 catgagcctg cccgagccgg tcggccgacc cgcgccgagc ttcggcatcc gaccgccacc
     2581 gcctcgggag attcagttgg aggtcaccgg cgcctgcaac cttcgctgca cgatgtgcct
     2641 ggtcgcctac cgccccaccc tgtcccggag cgaaggcagc atgtcgttcg agaccttccg
     2701 agcggtggtg gacgacgtgc cggggctgga gcggatcacc ctccaagggc tgggggagcc
     2761 cctgctcgcg ccggacctgt tccggatgat cgcctacgcg gcggagcgcg ggatcaggat
     2821 gggcttcaac accaacggga ccctgctcac gcgctcgcgc gccgtccgtc tggtgagggc
     2881 cgggctgggg tggctgcaca tctcggtgga cggtgccacc gccgagacct acgagagcat
     2941 ccgctccggg tcggacttcg accgggtgac caggaacgtg cggggattgg tggaggcgat
     3001 gcgggaacgc tcggcctccg agcccgagct ctccctggtc ttcgtcgcga tgcgccgaaa
     3061 cgttcgtcag ctccccgaca tggtccgcct ggcggcgtcc tggggggtcc cgcgactgtg
     3121 ggtacagaac ctgtctcact ccttcgccga cgtgagcccc atgaccgact accgggggat
     3181 cggcgagttc acagccgccg aggcgctgtg gacggaccgc gacggcgagg tggaggacct
     3241 gttcgaccgg gctcggcgcc tggccgaccg gctcgcgatc gagctgcggc tccctcggct
     3301 cgaggagcgg tccacgagcc gaggctcggg cacgcccggt tgcgactggc cgtggcggtc
     3361 cacgttcgtg aaccacgacg gcacggtcca gccgtgctgc atgctgatgg gggcggaccg
     3421 gggggtgctg ggatcggtcc ggaccgagcg gttcggcgag gtctgggacg gggccgggta
     3481 ccgggccttc cgacggcgcc tgctcagcga tcagccaccc gacgtgtgcc gtgggtgctc
     3541 gatgtaccgc ggggtgttct gaggctaccc cagggtcgcg ggcaccggga gccggaccgg
     3601 tggtgtgtcg atcggaacca cccggcctgc gacggggcgg tcggttcccc ggagctcccc
     3661 gcgcgtcgac cgccagagct cgtgtagggc gtcgaccacc gtgacgggct cgatgccgcg
     3721 cagacagcgg tggtggccct gcgggcagac gctccgatag cagaaccggc aggggacgtc
     3781 gtggatcagc acgcggctgg ggacccccca gggggtgtgc tgggggttcg tcagggcata
     3841 gaggaccacc acgggcgtgc ccaccccagc cgccaggtgg acgggcgccg tgttgttcga
     3901 cagcaggatc gaggcccgct cgatcagcgc ggcgagctcg gggaccgaga gctccccggc
     3961 cagggagtgg acgggcacgc cggcggtccg acgcaccgcc tcgaccaggt cccgctcggc
     4021 ctccgagccg gttagcacca
//
LOCUS       DASQQQ010000146         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_311511, whole genome shotgun sequence.
ACCESSION   DASQQQ010000146 DASQQQ010000000
VERSION     DASQQQ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311511"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VE646_06005"
     CDS             complement(<1..603)
                     /locus_tag="VE646_06005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZD79575.1"
                     /translation="MYKTKPSAACVMPADHHLRVLALNGSLKHRPALSNTEEVVEKVL
                     DEVREHASIDAEILRLADHRLPVGLEFRESQDDDWPGIVERIKASDIVLFATPIWWGG
                     RSSLMQRVIERLDALDEEYIATGRSALYNKTAGIVITGSEDGALSTMGTIMMVLTFMG
                     FTLPPECACYWVGEVGYPPAEDREKRLRNPAVPHMAKNMAR"
     gene            649..1398
                     /locus_tag="VE646_06010"
     CDS             649..1398
                     /locus_tag="VE646_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020497141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZD79576.1"
                     /translation="MESKGGRVRRAVPLPTLLDVLTNIIVHTSLAFMNDSQTDVASLQ
                     VLREPDGAAALLDPERRRLVEALQETPDSAAGLARRLGESRQRLNYHLHALEDAGLIE
                     LQEERRRGNCVERVLRVVARRFVLDPGALGGLAVDPAELTDRFSASYLIALAARAIRE
                     LADLIERAARGRKRLATGGIETQLRLSRPADFRVFLDDLSEAVAEVVARHHDERGGGR
                     AFRVLVGAYPAPEGADARAQPSEARDEGGEA"
     gene            1395..>2040
                     /locus_tag="VE646_06015"
     CDS             1395..>2040
                     /locus_tag="VE646_06015"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZD79577.1"
                     /translation="MSDEAKGGTDPRMAPAAEGTRSAEGQVEIGAPPERIWRALTEAE
                     ELIRWFPLEARVEPGEGGSVWMSWKNEYAGESKILVWEPGRHLRTSWGWHEGEGPVQI
                     TDYLLEGRGGRTVLRVVTSGFPSDASWDDWVEGTRKGWAFELASLRHYLEHHDGEDRE
                     VIYLRRRVGRTREEIWARLFGPHGLGGRPLGGRPIDEERPVQYAAIVDDPPGGLF"
BASE COUNT          292 a          618 c          805 g          325 t
ORIGIN      
        1 ccgggccatg ttcttcgcca tgtgcgggac ggcggggttc cggagccgct tctcacgatc
       61 ttccgccggt gggtagccga cctcgcccac ccagtagcag gcgcactccg gcggcagcgt
      121 gaaccccatg aacgtgagca ccatcatgat cgttcccatc gtggagagcg cgccgtcctc
      181 gctcccggtg atgacgatcc ccgcagtctt gttgtagagg gcgctgcggc cggtggcgat
      241 gtactcctcg tccagggcgt ccaaccgttc gatcacgcgc tgcatcaggc tggagcggcc
      301 gccccaccag atgggagtgg cgaagagcac gatgtcggag gccttgatcc gctcgactat
      361 gccgggccag tcgtcgtcct gggactcccg gaattcgagg ccgacgggga gacggtggtc
      421 ggccagtcgc aggatctcgg catcgatgga ggcgtgttcg cgcacctcgt ccagcacctt
      481 ctcgacgacc tcctcggtgt tggagagggc ggggcggtgc ttgagggatc cgttgagcgc
      541 gaggacgcgc agatggtggt cggcaggcat cacgcaggcg gcggagggct tggtcttgta
      601 catggccggc tccggggttt cgggtggaag cggcgggtgt tcgaagcggt ggaatctaag
      661 ggcggtcggg tccggcgggc agtgccgtta ccgacattgt tggatgtctt gacaaatatt
      721 attgtccata ctagcctggc gttcatgaac gattcacaga ccgatgtcgc gtcgctccag
      781 gtgctacggg agcccgatgg ggccgccgct ctgctcgatc cggaacgacg gcggctggtg
      841 gaggcccttc aggagacgcc cgactctgcc gccgggctgg cgcgccgact gggcgagtcc
      901 cggcagcggc tgaactacca cctccacgcc ctggaggacg ccggcctcat cgaactgcag
      961 gaggaacggc gccgcgggaa ctgcgtggag cgcgtgctcc gggtcgtggc gcgacggttc
     1021 gtgctggacc ccggggccct ggggggcctg gccgtggatc cggcggagct gaccgaccgt
     1081 ttctcggcct cgtacctgat cgcgctggcg gcccgggcga tccgggagct ggcggacttg
     1141 atcgagcggg cggctcgggg gcggaagcgg ttggcgaccg gcgggatcga gacgcagctg
     1201 cgcctctcca ggccggccga cttccgggtc ttcctggacg acctgtcgga ggcggtggcg
     1261 gaggtcgtgg cgaggcatca cgacgaacgc ggcggcggcc gcgcgttccg ggtcctggtc
     1321 ggcgcgtacc cggcgccgga gggggccgat gcgagggctc agcccagtga ggcgagagac
     1381 gaaggaggcg aggcatgagt gacgaagcga aggggggaac cgacccccgt atggcccctg
     1441 cggccgaggg gacgcgttcg gccgagggcc aggtcgagat cggcgcgccg cccgagcgga
     1501 tatggcgggc gctgacggag gccgaggagc tgatccggtg gttccctctg gaggcgaggg
     1561 tggagcccgg ggagggcggg tccgtctgga tgtcgtggaa gaacgagtac gccggtgagt
     1621 cgaagatcct ggtttgggag ccggggcgcc acctgcgtac gagctggggc tggcacgagg
     1681 gcgagggccc ggtccagatc accgactatc tgctggaggg gcgcggcggc cggacggtgc
     1741 tgcgggtcgt gacctcgggt ttcccgagcg acgcctcgtg ggacgattgg gtggagggca
     1801 cgcgaaaggg ctgggcgttc gagctggcct cgctgcggca ctacctggag catcacgacg
     1861 gcgaggaccg ggaggtgatc tacctacggc gtcgggtcgg tcggacccgg gaggagatct
     1921 gggcccggct cttcggcccg cacggtctgg gcggccggcc cctgggcggc cggcccatcg
     1981 atgaagagcg gcccgttcag tatgcggcga tcgtggacga tcccccgggc ggcctcttcc
//
LOCUS       DASQQQ010000147        13163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_395064, whole genome shotgun sequence.
ACCESSION   DASQQQ010000147 DASQQQ010000000
VERSION     DASQQQ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13163
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395064"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="VE646_06020"
     CDS             complement(<1..161)
                     /locus_tag="VE646_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018504523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HZD79578.1"
                     /translation="MGRTLPEKIWDRHVVRRAEGEPDLLYVDLHLVHEVTSPQAFDAL
                     RMAGRRVRRS"
     gene            259..1029
                     /locus_tag="VE646_06025"
     CDS             259..1029
                     /locus_tag="VE646_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004983042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZD79579.1"
                     /translation="MSLTGGAREGRVGVLDRAVRILDAVGEGHRTFTDIVGATGLPRP
                     TAHRLLKALEGHGLLASIDGRRYGPGPHLMRLAATAAKDQPLRAVAHPVLEQLAERIG
                     ESAQLYVRRGDVRVCVDAVEATSELRTIVPVGATLPLTAGSAGKLFLAWSTEEDRDRL
                     LRSMPRLTADTRGPDALRRELAEIRRRGWAASIGEREPGVASVSAPVHGPDGALAAAI
                     SVSGPYRRRGRFRPRSHAAALVAAARGIEESLGAGPAA"
     gene            complement(1043..2779)
                     /locus_tag="VE646_06030"
     CDS             complement(1043..2779)
                     /locus_tag="VE646_06030"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HZD79580.1"
                     /translation="MSLAVSLVVSTAVGAPAAVDPEPTLLVSPAIVTAGGGVVVSGEL
                     EGGDGCGAGRPADLGWRPARGTWTTLAETTTGDDGSFAYNDAPATSGSYQVSFPAFDG
                     CGSLVSGSVQVRVRTRVEASLQASSPVRAGSCLSVSTEVSPPKPGQEVVLQQRTSSGW
                     VTLRSLVLDAGSSATDRPCFGFADLGIVRLRVRWPTQDAMNVTGTSSTLAFKVEKARW
                     MRRIDRLIDHRSVSLSVDRDGATLYEHAPDTRRIPASNEKLLLSMALLDRLGPGHRMS
                     TSAAAASVDHGVVRGDLWILGRGDPEIRASRMAALARRLVEAGLRRVSGQVLGAVGYF
                     RHDWWASGWRTNARAYVAPPTALVFDGNRTGDGYTRSPERLAARSLTRQLRLRGVSVV
                     GRPGMGPAPSGLIRLAAIRSRPLRQLIARMDRPSDNFFAEVLGKLLGVSAEGAPGTIA
                     KGARALRAWVSSHGERFSLYDGSGLSYDNRVSAGGMVRLLEAAGARPWGAVLRQALPA
                     GGQGTLEHRLVGERVRAKTGTLDGVSALSGWVWLDEDDGWAEFSILSSLPKWKAVDIE
                     DGIVRTLAEHAR"
     gene            2914..3774
                     /locus_tag="VE646_06035"
     CDS             2914..3774
                     /locus_tag="VE646_06035"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HZD79581.1"
                     /translation="MAYLEFSFRPEVSGPILVCAFAGWNDGGEAASTALRYARERWGA
                     RRFARIDPEEFFDFQVNRPTVRLDEGQTRRLDWPACEFFHARVGGRDLIVFLGVEPNV
                     RWRTFCEQILAVSRDLGVRLLVTLGAFLADVPHTFPTPVTAASSDPEWSSRLDVEPSR
                     YEGPTGILGVLNEMASRTGVPALSLWGAASHYLPSGTNPKVALALLEKLGELLEVPID
                     LAGMRTATAVWDQQVRRAIEEDEDLVAYVRRLEELTEEGRDLGEIPSGDQLADELERF
                     LRDQRGDGGS"
     gene            3774..4568
                     /locus_tag="VE646_06040"
     CDS             3774..4568
                     /locus_tag="VE646_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZD79582.1"
                     /translation="MELRPLGTTGHRVPVVGMGTWKVFDVPPQEEDRIGGVIRAGFEA
                     GVRVIDSSPMYGRSEEVLGHALAGLRTEIFVATKIWTASGEEGRRQLRRQLGLYEGSV
                     DLEQVHNLMGWREHLDWLEGERDAGRVRLLGATHWNPAAFDELEQVMRTGRIHAVQIP
                     YNPLEAQAGERILPLAEELGLGVIVMSPFDEGSLLPGAEPEALEELGVQTWAQALLKW
                     SLSDPRVHVVIPATSRPEHAVQNAAAGQPPWFDRDQRGLVSRLAGA"
     gene            4565..5326
                     /locus_tag="VE646_06045"
     CDS             4565..5326
                     /locus_tag="VE646_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HZD79583.1"
                     /translation="MNARAAAWPGSAHELEAEQRRLATLRPKPWTWVGPARVGGTFVA
                     FSTSGSEVPVERSWAVAVVVEPGAEAGPPDRAVAVAEVTEPYRAGYLALREGSLLEAA
                     ARALARRPDVLLVNATGRDHPRRAGLALHLGAVLDLPTVGVTDRPLLAGGPMPGDERG
                     CSEPLRLGDVVVGFRVRTRRGVRPVCAHAGFRTDPATARDVLLASTRGTRTPEPLRQA
                     RFLARSARARDEGRTPPGWTQDRSSRVTLPPVRRA"
     gene            5358..6659
                     /locus_tag="VE646_06050"
     CDS             5358..6659
                     /locus_tag="VE646_06050"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HZD79584.1"
                     /translation="MTRLRTVIERFRGNLFFVPALFLTAAIALAEGAITLDVTLHRAA
                     FQLPVGLASTVASARSLLGTVASATIAFAGIAFSVSLLMVQLASTQYSPRVIYGFLRD
                     PFGKRVMGLVIGTFTFCLLVLLVVHAPAGSGVPVVPQVSVLIAVVLGVSSIVAVVALI
                     SHGVISMQAGEIVRRITDEAREQIERECAPVAAPPRRLDRVVPDGPGLIVRAPGDGWV
                     QTVDIDRLLGAVPEGGVVRLETWPGAFVPEETPICTVWPMPEDVRATARTMERAFVIG
                     RSRTMQQDLTFGIRQLVDIALRALSPGVNDPTTANSAIVHIGAVLRSLLLHDLPSLVL
                     TDGGRRVIRPREFEHRDYVALGFDEIRRAGAGQPAVAESLLQVLGMLAAALGTAGLRE
                     RIEPLRAQARLVVEVAERSDLPNADLDRIHRAGVRAGLSYR"
     gene            6785..7210
                     /locus_tag="VE646_06055"
     CDS             6785..7210
                     /locus_tag="VE646_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79585.1"
                     /translation="MEPDQDSEVRERGMGETAIFARYVAAMLRIGQADPDRARAAILA
                     RGDQVLAALRDAMQPGATDSVRIVALIDMYLQTTLEMLYAASRSDDRLEFAEAEMKAW
                     AVAGALDRAAEAARSKDRDTVYAAMRAAESVLGINLTSS"
     gene            7288..8550
                     /locus_tag="VE646_06060"
     CDS             7288..8550
                     /locus_tag="VE646_06060"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD79586.1"
                     /translation="MNGVRTAVASTFSSLRIRNFRLFITGQFLSGIGTWMQMVAAPWL
                     VLQLTGSGVALGIDTALQYAPMLVFGAWGGVIADRFDNRRLQILTQLAYAVTALALWT
                     IVATGVVRIGMVYALSLLTGLVTAVDMPTRQSFYLEMVGRTDLTNAMSLNTATFTGTR
                     IVGAALAGVLIALIGTAPIFLINGITYLAVVVALLRMRVGELNHREPVPRARGQIREG
                     VVHVWRTAELRLPLLLMAAVFVFSFNFSVLLPLFATRTFAGGSQTYGAMLSLWGVGSL
                     AGALVLAGRSADANAHRLSMFGLALGALSLAVSLAPAIPLAYVLLPPLGAVGISFAIT
                     GNSTLQLRSAPHMRGRVMALYSVVFLGSTPIGGPLSGWVGQHLGARVGLAGGGAIALA
                     AAGLAASRLRSGSEPKGRSVAAGGYSHR"
     gene            8559..9560
                     /locus_tag="VE646_06065"
     CDS             8559..9560
                     /locus_tag="VE646_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HZD79587.1"
                     /translation="MRALVLEAPRTHLVEREVPVPRPGPGQVLLRVSACGVCRTDLHV
                     VDGELTSPELPLIPGHQIVGVVEEVGPGVLGVAERVGVPWLGWTCGECRYCRGGRENL
                     CPVARFTGYQIDGGFAEYAVADERFCLPIPDDYPDLQAAPLLCAGLIGWRALKMTGDA
                     ERLGIYGFGAAAHIVAQVARHQGRRVYAFTRPGDERTQAFARELGAAWAGASTQPPPE
                     ELDGAILFAAAGELVPAALEAVAPGGTVVCAEIHMTDIPSFPYDLLWREKELRSVANL
                     TRADGREFLELAPRVQVRTTVTTFPLEQANDALDALRGGGLQGAAVLVVRPVEPTEA"
     gene            complement(9712..10809)
                     /gene="serC"
                     /locus_tag="VE646_06070"
     CDS             complement(9712..10809)
                     /gene="serC"
                     /locus_tag="VE646_06070"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HZD79588.1"
                     /translation="MKQRVWNFSAGPAALPLPVLEQIRSDLPVLPGAGMSVLEMSHRS
                     DRFGQILGEAEQGLRELLHVPDGFRVLFLQGGASLQFSMAPMNLLPEGGPPGAYVLTG
                     SWGAKAIAEARKQGPVTAAWDGESTGFTSVPNLPRLSGLSVSSYLHVTSNETIQGVQF
                     PSGFEPASGPPLLCDASSDFLSRPLPLERYGLLYAGAQKNAGPAGLTVVLIADALLER
                     IPDGLPSMLDYRTYAASGSLYNTPPVFTIYVLMLVTRWLRDQVGGLEEMQARNRHKAE
                     LLYQAIDGSGGFYRGHALPEARSTMNVTFRLPDASLEEAFVDAAAGQGLVGLKGHRSV
                     GGIRASIYNAMPLEGVQTLRTFMERFPARHG"
     gene            complement(10883..11731)
                     /locus_tag="VE646_06075"
     CDS             complement(10883..11731)
                     /locus_tag="VE646_06075"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HZD79589.1"
                     /translation="MTGIQDQVGALRRVYVRAPQAEDHLAWRAYGWHAPPHPSRAAEE
                     HAAFRAELERAGSEVVSGLAPAPGDPDAIYVYDPVLATTSGALLLRPGKEGRRSEPDV
                     VAQDLRRAGMEIRGRIEPPGTIEGGDLLWLDEGTLAVGRSYRTDERGVRALRSALPDV
                     EVLAFDLPHRRGPGSVLHLLSLISLLDRDLAVAYPPLVPVRLMQELARRGITLVEVPE
                     AELESMGPNVLALAPRVALALEGNPETRRRMEASGVEVRTYRGDEISRKGDGGPTCLT
                     RPLLRD"
     gene            complement(11738..12850)
                     /locus_tag="VE646_06080"
     CDS             complement(11738..12850)
                     /locus_tag="VE646_06080"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HZD79590.1"
                     /translation="MSWPVVRSFGAPGDRGQAYGAQASERIRRSIDLYTRVFRHYADL
                     GWEEVRTRARGFLAPIEGYDPSALLELDGIAGGAGVELDDILALNVRTEVMFGLGIGA
                     RECTAVVALPDATTDGHVILAQNWDWKPQTQETCVLLVSAPDDGPAFVTLVEAGLLAK
                     TGMNEAGIGLATNALISDRDQGEPGVPYHAILRRILQADSFAEAANAVFRPERASSAN
                     YLVAHRDGEALDLEAAPGRAAETCLIWPEMGVLAHANHFQSTRFMFRDTGLADEGADS
                     LVRQHRAERAMREGAGTLSVSSIQEALRDHFDRPSSVCSHPTPGTDPEEDYATIASVV
                     MDLSVGTLWVTEGTPCRAQFERYDVAQLFSEVRAGS"
     gene            12894..>13163
                     /locus_tag="VE646_06085"
     CDS             12894..>13163
                     /locus_tag="VE646_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZD79591.1"
                     /translation="MWPGDARVAVAVTFDLDAEEVWIADDAANRHRPAALSQGVYGPK
                     VAVPRIVDLLERHGIRATFFVPGRVAEAHPVAIEALVAGSQELACH"
BASE COUNT         1790 a         4555 c         4914 g         1904 t
ORIGIN      
        1 gatcggcgga cccggcgccc cgccatgcgc agggcgtcga aggcctgggg gctggtcacc
       61 tcgtgcacga ggtgcaggtc cacgtagagg aggtcgggct caccctcggc ccgccggacc
      121 acgtgtcggt cccagatctt ctccggcaag gtccgtccca tcgttccccc ctcgggggcc
      181 gaccggtatc cacgtctgtc gactggccgc ccgcctcggt cggtcccata tcgtggaacc
      241 tggtatcaat atgtgggcat gagtctaaca ggaggagcgc gcgaagggcg ggtcggcgtc
      301 ctggaccggg cggtgcgcat cctggacgcc gtgggggagg gccaccggac gttcacggac
      361 atcgtcggtg ccaccggact cccccgtccc acggcccaca ggctgctcaa ggccctcgag
      421 ggccatgggc tgctcgcctc gatcgacggc cgccggtacg ggccgggtcc gcatctgatg
      481 cgcctggcgg ccacggccgc gaaggaccag ccgctgcgcg ccgtggccca cccggtgctc
      541 gagcagctgg cggagcggat cggcgagagc gcgcagctct acgttcgtag aggcgacgtt
      601 cgggtctgcg tggacgcggt cgaggcgacg agcgagctcc ggacgatcgt gcccgtcggg
      661 gccacgctgc cgctcacggc gggatccgcg gggaagctct tcctggcctg gagcaccgag
      721 gaggatcggg accggctcct ccgaagcatg ccccggctga ccgcggacac gcgcggaccg
      781 gacgcgctcc gccgggagct cgccgagatc cggcgacggg gatgggcggc gagcatcggg
      841 gagcgcgaac ccggcgtggc ttcggtgagc gcgccggtgc acggtccgga cggcgcgctg
      901 gcggcggcga tctccgtctc agggccgtac aggcggcgcg gtcggttccg cccccgctcc
      961 catgccgcgg ccttggtggc ggcggcacgg gggatcgagg agtcgctcgg ggctgggccc
     1021 gcggcctgag gccagcccgg gatcatcgcg cgtgctcggc cagggtgcgg acgatgccat
     1081 cctcgatgtc cacggccttc cacttcggga gcgaggacag gatcgagaac tccgcccagc
     1141 cgtcatcttc gtccagccac acccatccgg agagggccga cacgccgtcc agggttccgg
     1201 tcttggcacg gacccgctca ccgacgaggc ggtgctcgag cgttccctgc ccaccggccg
     1261 gcagagcctg tcgcagcacc gctccccagg gccgcgcccc ggctgcctcc aggagacgga
     1321 ccatcccccc ggcgctcacg cggttgtcgt aggacagacc ggatccgtcg tacaggctga
     1381 accgctcccc atggctcgag acccaggcac ggagcgcacg ggcacccttg gcgatggtcc
     1441 ccggggctcc ctcggcggac acgccgagga gcttgcccag cacctcggca aagaagttgt
     1501 cggagggccg atccatcctc gcgatcaact gacggagggg tcgggagcgg atcgcagcca
     1561 gcctgatcag gccggagggc gcgggtccca tcccgggccg tccgaccacc gagacccccc
     1621 gcagccggag ctgccgcgtg agggaccggg cggccaggcg ctcgggcgaa cgtgtgtagc
     1681 catccccggt ccggttgccg tcgaagacca gggcggtcgg gggcgccacg tacgcccgcg
     1741 cgttggtccg ccaacccgac gcccaccagt cgtgacggaa gtacccgacc gcccccaaga
     1801 cctgcccgct cacccgccgg aggcccgcct ccaccaggcg acgggcgagg gcggccatcc
     1861 gggaggcgcg gatttcggga tcgcctcgcc ccaggatcca cagatcgccg cggaccacgc
     1921 cgtgatcgac cgacgcggca gccgccgacg tggacatgcg gtggcccggg ccgagccgat
     1981 ccagcaaggc catcgacagc agcagcttct cgttggacgc ggggatccgc cgcgtgtcgg
     2041 gagcatgttc gtacagggtg gcgccgtccc ggtccaccga cagggacacg gaccggtggt
     2101 cgatcaggcg gtcgatccgc cgcatccacc tggccttctc caccttgaac gcgagcgtcg
     2161 atgaggtccc cgtcacgttc atcgcgtcct gggtgggcca tcggacccgg aggcggacga
     2221 tgcccaggtc ggcgaagccg aagcaggggc ggtccgtggc ggacgagcct gcatccagca
     2281 ccagggaccg cagcgtcacc cagcccgagg acgtgcgctg ctggagcacc acctcctgcc
     2341 caggcttggg agggctcacc tccgtggaca cggacagaca gctccccgcg cgcaccggtg
     2401 aagacgcctg gagcgaggct tcgacgcggg tccgcacgcg cacctgcacc gatcccgaga
     2461 ccagggatcc gcatccatcg aaggcgggga acgagacctg gtaggaaccc gaggtggccg
     2521 gcgcgtcgtt gtaggcgaac gagccatcgt cgccggtggt tgtctcggcg agcgtggtcc
     2581 aggtgccccg ggcgggccgc cagcccagat cggcaggacg cccggccccg cacccgtcgc
     2641 caccttcgag ctcgcccgat accacgacac ccccgcctgc ggtgacgatg gcgggagaga
     2701 ccaggagggt cggctcgggg tccacggcgg ccggcgctcc cacagcggtg gacaccacga
     2761 gggacaccgc gagggacacc gcgagcgacg cggcgacgga tagacgagcg aacttcaggg
     2821 tcctctcctc ggatgggctc gctgattctg tcccggcggg tggtccccac tcaaggaccc
     2881 cagacgtcgg gtcggcggcg gtagcctgtc ctggtggcgt acctggagtt ctccttccgc
     2941 ccggaggttt cggggccgat cctcgtgtgt gccttcgcgg ggtggaacga cggcggagag
     3001 gcggccagca ccgcccttcg ctacgcgcgg gaacgctggg gagcacggcg gttcgcgcgc
     3061 atcgatcccg aggagttctt cgacttccag gtgaaccgcc ctaccgtccg cctggacgag
     3121 ggccagaccc gccggttgga ctggcccgcg tgcgagttct tccacgcacg cgtgggagga
     3181 cgggacctca tcgtgttcct gggggtggag cccaacgtgc ggtggcgcac cttctgcgag
     3241 caaatcctgg cggtgagccg agacctcggc gtccggttat tggtgaccct gggggccttc
     3301 ctggccgacg tcccgcacac gttcccaacc ccggtcacgg cggccagctc ggatccggag
     3361 tggtcctcgc ggctggacgt ggagccctcc cggtacgagg gcccgaccgg catcttgggg
     3421 gtgctgaacg agatggcttc ccgcaccggg gtccccgccc tctcgctatg gggggccgcc
     3481 tcgcactacc tgccgtccgg cacgaacccc aaggtggcgc tggcgctgct cgagaagctc
     3541 ggcgagctac tggaggtgcc gatcgacctg gccgggatgc gcaccgcgac ggcggtgtgg
     3601 gaccagcagg tgcgccgagc catcgaggag gacgaggacc tggtcgccta cgtccggcgg
     3661 ctggaagagc tcaccgagga gggccgcgac ctgggggaga tcccatcggg cgatcagctc
     3721 gcggacgagc tggagcggtt cctgcgcgac caacgggggg acggggggtc ctgatggagc
     3781 tgcgccccct cggaaccacc ggccatcggg tgccggtggt gggcatgggg acctggaagg
     3841 tcttcgacgt tcctccccag gaggaggaca ggatcggagg ggtgatccgc gccgggttcg
     3901 aggccggggt ccgggtgatc gactcctcgc cgatgtacgg acgttccgag gaggtgctcg
     3961 gtcacgccct cgcggggctg cggaccgaga tcttcgtggc cacgaagatc tggacggcgt
     4021 cgggtgagga ggggcgacga cagctccgcc gtcagctcgg cctgtacgag gggtcggtgg
     4081 acctggagca ggtgcacaac ctgatggggt ggcgagagca tctggactgg ctggagggcg
     4141 agcgcgacgc gggccgggtg cggctgctgg gcgcgacgca ctggaacccg gcggccttcg
     4201 acgagctcga gcaggtgatg cggaccggac gcatccatgc ggtgcagatc ccctacaacc
     4261 cgctcgaggc ccaagcgggt gagcggatcc tgccgctggc ggaggagctg ggtttgggcg
     4321 tgatcgtgat gagcccgttc gacgagggga gcctgctgcc gggggccgaa ccggaggcgc
     4381 tggaggagct gggcgtgcag acctgggcgc aggcgctgct gaagtggtcg ctctcggacc
     4441 cgcgggtcca cgtcgtcatc ccggccacct ctcggccgga gcacgcggtc cagaacgcgg
     4501 cggccggcca gccgccgtgg ttcgaccggg accagcgcgg gctggtgtcg cgcctggctg
     4561 gcgcgtgaac gctcgggccg cggcctggcc tgggtcggcc cacgagctcg aggccgaaca
     4621 gcgccggttg gcgaccctgc gtcctaagcc ctggacctgg gtcggcccgg cgcgggtcgg
     4681 aggcacgttc gtcgccttct ccacgtccgg gagcgaggtc ccggtggagc ggtcctgggc
     4741 cgtcgccgtg gtcgtggagc ccggagccga ggccggcccc cccgatcgag cggtggcggt
     4801 cgccgaggtg accgagccgt accgggcggg gtacctcgcg ctgcgcgagg gctcgttgct
     4861 ggaggcggcg gcgcgcgccc tggcgcgacg cccggacgtg ctgctggtga acgccaccgg
     4921 acgggatcat ccccgacggg ccggcctggc cctgcatctg ggggcggtgc tggatctgcc
     4981 caccgtgggg gtgacggatc gccccctgct cgcaggtggt ccgatgccgg gagacgagcg
     5041 ggggtgctcc gagccgctcc ggctgggcga cgtggtggtc gggttccgcg tgaggacccg
     5101 tcgaggggtc cgaccggtgt gcgcgcacgc cgggttccgt accgacccgg ccacggcccg
     5161 ggatgtgctc ctggcctcga cgcgcgggac ccggaccccg gagcccctcc gccaggcgcg
     5221 gttcttggcc cggtcggccc gcgcccgaga cgagggcagg acgccacccg gctggaccca
     5281 ggaccgctcc tcaagggtga cgctcccacc cgtgcggcgc gcgtagggtc ggggctgacg
     5341 gggcaagagg ggacacgatg acgcgtctgc gcaccgtgat cgaacggttc cgaggcaacc
     5401 tgttcttcgt ccccgccctc ttcctgacgg ccgcaatcgc tctggcggaa ggggcgatca
     5461 cgctggacgt gaccctgcac cgggccgcgt tccagctccc ggtcggcctg gcctccacgg
     5521 tggccagcgc ccgatcgctg ctggggacgg tggccagcgc cacgatcgcg ttcgccggga
     5581 tcgcgttctc ggtctccctg ctgatggtcc aactggcttc cacccagtac tcgccgcggg
     5641 tgatctacgg cttcctgcgg gacccgttcg gcaagcgggt catggggctc gtgatcggga
     5701 cgttcacctt ctgcctgttg gtgctgttgg tggtccacgc gccggcggga tccggggtcc
     5761 ccgtggtgcc ccaggtctcg gtgctgatcg cggtggtact gggcgtgagc tcgatcgtcg
     5821 ccgtcgtggc gctgatcagc cacggcgtga tctcgatgca ggccggcgag atcgtccgtc
     5881 ggatcacgga cgaggctcgg gagcagatcg agcgcgagtg cgctccggtc gcggctccgc
     5941 cccggcggct cgaccgcgtg gtccccgacg gaccggggct catcgtgcga gcaccggggg
     6001 acgggtgggt gcagacggtc gatatcgatc ggttgttggg ggcggttccg gagggcggcg
     6061 tggtccgact cgagacctgg ccgggcgcct tcgtccccga ggagaccccg atctgcacgg
     6121 tctggccgat gcccgaggac gtacgcgcca ccgcccgtac gatggagcga gccttcgtca
     6181 tcgggcgctc acgcacgatg cagcaggacc tgaccttcgg gatccgccag ctggtggaca
     6241 tcgcgttgcg cgcgctctca ccgggggtga acgatcccac caccgcgaac tcggcgatcg
     6301 tccacatcgg ggcggtgctg cggtcactgc tccttcacga tctgccctct ctagtcctga
     6361 ccgatggggg gcgccgggtg atccgaccgc gcgagttcga gcatcgcgac tacgtggccc
     6421 tgggcttcga cgagatccgg cgggccgggg cgggtcagcc cgcggtggcc gagtccctgc
     6481 tgcaggtcct gggcatgctc gccgccgcgc tcgggacagc cgggctccgc gagcgcatcg
     6541 agcccctcag agcgcaggcc cggctggtcg tggaggtcgc cgagcgcagt gacctcccga
     6601 acgcggatct cgaccgtatc caccgggccg gtgtccgggc cggcctgagc taccgctgag
     6661 cccggtgggc gccggggcga ggtcgacggc gggaaataga ccttcgaccg agcctcggac
     6721 atcgaacggc cgatggcggg tatccccctg ttggccgagg atgcggtggt gagggggacg
     6781 agacatggag ccggatcagg acagcgaggt gcgggagagg ggcatgggtg agacggcgat
     6841 cttcgcccgg tacgtggcgg cgatgctccg gatcggtcaa gccgacccgg atcgggcccg
     6901 agcggcgatc ctggcgcggg gtgaccaggt cctggcggcc ctccgcgacg cgatgcagcc
     6961 cggtgcgacg gattcggtcc ggatcgtcgc cctcatcgac atgtacttgc agacgacgct
     7021 cgagatgctc tacgccgcgt cgcgttccga cgatcggctg gagttcgcgg aagcggagat
     7081 gaaggcctgg gccgtcgcgg gcgcgctcga tcgcgccgcc gaggcggcca ggtccaagga
     7141 ccgggacacc gtctacgcgg ctatgcgcgc ggccgagtcg gtgctcggga tcaacctcac
     7201 ctccagctga ggacgagggg ggcagcccac gaaccaccca tgggggtcgt gggctgcgct
     7261 ccatcgggtc accgtaggct gttggggatg aacggcgtgc ggaccgcggt ggcctcgacc
     7321 ttctcctcgc tccggatccg caacttccgg ctgttcatca ccggtcagtt cctctccggc
     7381 atcgggacct ggatgcagat ggtggcggca ccgtggctgg tgctccagct caccggcagc
     7441 ggggtggccc tcgggatcga cacggcgctc cagtacgcgc cgatgctggt gttcggggcg
     7501 tggggaggcg tgatcgccga ccggttcgac aaccgccgcc tccagatcct gacccagctc
     7561 gcctacgcgg tgacggcgct cgccctctgg acgatcgtgg ccaccggggt ggtgcggatc
     7621 gggatggtgt acgccctgtc gttgctcacc ggcctggtga cggccgtcga catgccgacc
     7681 cggcagagct tctacctcga gatggtcggc cggaccgatc tcacgaacgc catgagcctg
     7741 aacaccgcca ccttcaccgg gacacgcatc gtgggggccg cgctggccgg cgtgctgatc
     7801 gctctgatcg gtaccgcacc gatcttcctg atcaacggca tcacctacct cgccgtagtg
     7861 gtggcgctgc tccggatgcg ggtgggcgag ctgaaccacc gggagccggt ccccagggcc
     7921 cgggggcaga tccgcgaggg cgtcgtgcac gtctggcgga ccgccgagct gcgcctgccc
     7981 ctgttgctga tggccgcggt cttcgtcttc tcgttcaact tctccgtcct gctgccgctg
     8041 ttcgccaccc ggaccttcgc tggtggatcc cagacctacg gcgccatgct ctcgctgtgg
     8101 ggcgtgggct ccctggccgg ggcgctcgtc ctggccgggc ggtcggcgga cgcgaacgcc
     8161 caccggctgt cgatgttcgg tctggcgctg ggggcgctca gcctcgccgt gtcgctggct
     8221 ccagcgatcc cactcgcgta cgtgcttctg ccgccgctgg gggcggtcgg catctcgttc
     8281 gcgatcaccg ggaactcgac gctgcagctg cggtcggcgc cgcacatgcg gggtcgggtc
     8341 atggcgctgt actcggtcgt cttcctgggc agcaccccga tcggcggtcc gctctcgggg
     8401 tgggtggggc agcacctcgg cgctcgcgtg gggctggccg gaggtggggc gatcgccctg
     8461 gcggccgcgg gcttggcggc ctcgcggctg cggtcgggga gcgagccgaa gggtcgatcc
     8521 gtggcagccg ggggatactc gcatcgatga ccgatacgat gcgcgccctg gtcctggagg
     8581 cgccccgcac gcacctcgtg gagcgcgagg tcccggtgcc gcgcccggga cccgggcagg
     8641 tcctgcttcg ggtcagcgcc tgcggtgtct gtcgcacgga cctgcacgtg gtggacgggg
     8701 agctgacctc ccccgagctc ccgctgatcc ccggccatca gatcgtgggg gtggtggagg
     8761 aggtcggacc cggtgtgctc ggcgtggccg agcgggtcgg cgttccctgg ttgggctgga
     8821 cgtgcggcga gtgccggtac tgccggggtg ggcgcgagaa cctgtgcccg gtcgcccggt
     8881 tcaccggcta ccagatcgac gggggcttcg ccgagtacgc ggtggccgac gagcggttct
     8941 gcctcccgat ccccgacgac tacccggacc tgcaggcggc tccgttgctg tgcgcggggc
     9001 tgatcggctg gcgggccctg aagatgaccg gcgacgccga gcgactgggc atctacgggt
     9061 tcggggccgc ggcccacatc gtggcgcagg tggctcgaca ccaggggcgg cgcgtgtacg
     9121 ccttcacccg gccgggtgac gagcgaacgc aggccttcgc ccgagagctg ggggccgcgt
     9181 gggccggtgc ctccacccag ccgccgccgg aggagctgga cggcgcgatc ctgttcgcgg
     9241 cggcgggcga gctggtgccg gcggccctgg aggcggtggc gcccggtggc acggtggtct
     9301 gtgccgagat ccacatgacc gacatcccga gtttcccgta cgacctgctg tggcgggaga
     9361 aggagcttcg atccgtggcc aacctcacca gggcggacgg gcgggagttc ctcgagctcg
     9421 ccccccgggt gcaggtgcgg accaccgtga cgaccttccc gctggagcag gccaacgacg
     9481 cgctggacgc cctgcgaggg gggggcctgc agggggcggc ggttctggtg gtgcgcccgg
     9541 tggagccgac cgaggcctag ggccgggggc tcgagcgccg ggatgcccgt gcggtaggtg
     9601 tcgcaggccg cggcctgagc ccccggccca cgcccgagct gcgacctccc gctcgagcag
     9661 cgccccgccc gcaggtcctt tccccctcgc aggtgacgga cgcggctcgg ctcagccgtg
     9721 ccgagcgggg aacctctcca tgaaggtgcg cagggtctgc accccctcga gcggcatggc
     9781 gttgtagatc gatgcccgga tcccacccac cgaacgatgg cccttcaatc ccaccaggcc
     9841 ctggccggcg gccgcgtcca cgaaggcctc ctccaggctg gcatcgggca gccggaacgt
     9901 gacgttcatc gtggaacggg cctcgggaag ggcatgcccg cggtagaagc cgccgctgcc
     9961 atcgatggcc tggtagagca gctcggcctt gtgccggttg cgcgcctgca tctcctccag
    10021 gccgcccacc tggtcgcgga gccagcgcgt caccaacatc agcacgtaga tcgtgaacac
    10081 gggcggggtg ttgtacaggg atccgctggc ggcgtacgtg cggtagtcga gcatggaggg
    10141 gaggccgtcg gggatccgct ccagcagggc atcggcgatc agcaccacgg tgaggcccgc
    10201 cggaccggcg ttcttctggg cccccgcgta caggagcccg taccgttcga gcgggagcgg
    10261 gcgtgagagg aagtccgagg aggcatcgca gaggagcggc ggccccgagg ccggctcgaa
    10321 ccccgacggg aactgcaccc cctggatcgt ctcgttcgag gtcacgtgca ggtaggagga
    10381 gacggacagg ccgctcagcc tcggcaggtt cggaaccgag gtgaagccgg tgctctcgcc
    10441 gtcccacgcc gcggtcaccg gaccctgctt gcgggcctcg gcgatcgcct tggcgcccca
    10501 ggagccggtg aggacgtacg cgcccggagg gccgccctcc ggcagcaggt tcatgggcgc
    10561 catggagaac tgcagcgagg cccccccttg gaggaagagc acgcggaagc catccggcac
    10621 atggaggagc tcccgcagcc cttgctccgc ctccccgagg atctggccga accggtcgga
    10681 gcggtgactc atctcgagga cggacatccc cgctccgggc agcaccggga gatccgagcg
    10741 gatctgctcc aggaccggca gcggcagggc ggcgggcccc gccgagaagt tccacacgcg
    10801 ctgtttcatc tgcccatgct cccgcgccgg gactccgagc gtcacgccgc acacgccgaa
    10861 gggggcggag tgatcggccc ggtcagtcgc ggagcagcgg tcgggtcagg caggtcggac
    10921 cgccgtcgcc cttccgtgag atctcgtccc cccggtaggt ccgaacctcc acccccgacg
    10981 cttccatgcg ccgccgggtc tcggggttcc cctccagcgc cagggccacc cgaggggcca
    11041 acgccagcac gttcggtccc atcgactcga gctcggcctc cgggacctcc accagggtga
    11101 tgccccgacg cgcgagctcc tgcatcagcc ggaccggcac gagtggtggg taggcgaccg
    11161 cgaggtcccg atccagcagg ctgatcagcg agagcagatg gaggaccgat ccgggccccc
    11221 gccggtgcgg cagatcgaag gcgaggacct ccacgtcggg cagcgcgctg cgcagggcac
    11281 ggacgccccg ttcatcggtc cggtagctgc ggcccaccgc cagcgtcccc tcgtccagcc
    11341 acaggaggtc gccgccctcg atcgtccccg gtggctcgat ccggccgcgg atctccatcc
    11401 ccgcccgccg gagatcctgg gcgaccacat cgggttcgga gcgtcgtccc tccttgccgg
    11461 gccggaggag cagcgctccg gaggtcgtgg ccaggacggg gtcgtagacg tagatcgcgt
    11521 ccggatcgcc gggcgcgggg gcgagacccg ataccacctc cgagcccgcc cgttcgagct
    11581 ccgctcggaa cgcggcgtgc tcctcggccg cccgcgatgg gtggggaggc gcgtgccagc
    11641 cgtaggcgcg ccaggccagg tggtcctcgg cctggggggc tcgaacgtac acgcgccgca
    11701 gggcgccgac ctggtcctgg atgcccgtca cgtccgtcta cgagccggca cggacctcgg
    11761 agaagagctg cgccacgtcg tatcgctcga actgcgcccg gcacggcgtg ccctcggtga
    11821 cccacagggt tccgaccgac aggtccatca cgaccgaggc gatggtggcg taatcctcct
    11881 ccgggtcggt cccgggggtg gggtgggagc acacggacga gggccggtcg aagtggtcgc
    11941 gcagcgcctc ctggatcgag ctcacggaga gcgtcccggc gccctcccgc atggcgcgct
    12001 cggcccggtg ctggcgcacc aggctgtcgg cgccctcgtc ggccaggccc gtgtcgcgga
    12061 acatgaagcg ggtgctctgg aagtggttgg cgtgggccag gacacccatc tcgggccaga
    12121 tcaggcaggt ctcggcggcc cgtccgggag cggcctcgag atccagcgcc tcgccgtcgc
    12181 ggtgcgcgac caggtagttg gcggaggagg cgcgctcggg gcggaacacg gcgttcgcgg
    12241 cctccgcgaa cgagtccgcc tgaaggatcc gacgcaggat cgcgtggtag gggacgccgg
    12301 gctcgccctg gtcgcgatcg gagatcaggg cgttcgtggc cagcccgatc cccgcttcgt
    12361 tcatgccggt cttcgccagg agcccggcct cgaccagcgt cacgaaggca gggccgtcgt
    12421 cgggggcgga caccagcagc acgcacgtct cttgcgtctg gggcttccag tcccagttct
    12481 gcgccaggat cacgtggccg tccgtggtcg cgtcgggcag cgccaccacg gcggtgcact
    12541 ccctggcccc gatcccgagc ccgaacatca cctccgtgcg gacgttgagg gcgaggatgt
    12601 cgtccagctc cacccccgct cccccggcga tgccgtcgag ctcgagcagg gcggaggggt
    12661 cgtatccctc gatgggggcc aggaaccccc gggcccgggt ccgcacctcc tcccatccca
    12721 ggtccgcgta gtggcggaag acgcgcgtgt agaggtcgat ggatcggcgg atccgctccg
    12781 aagcctgggc accgtaggcc tgtccccggt ctcccggagc gccgaaggac cgcaccacgg
    12841 gccagctcat ggcgcgatga tagggcctcg gcgagccggg gaggcgagga tcgatgtggc
    12901 cgggagatgc gcgggtggcg gtggccgtca cgttcgacct cgatgccgag gaggtgtgga
    12961 tcgccgacga tgcggccaac cgccatcggc cggcggctct ctcgcagggc gtctacgggc
    13021 ccaaggtcgc ggtgccccgc atcgttgatc tgctggaacg gcatgggatc cgggcgacgt
    13081 tcttcgtccc cgggcgagtg gccgaggccc atccggtcgc gatcgaggcg ctggtggccg
    13141 ggagccagga gctcgcctgc cac
//
LOCUS       DASQQQ010000148         7029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_177520, whole genome shotgun sequence.
ACCESSION   DASQQQ010000148 DASQQQ010000000
VERSION     DASQQQ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7029
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177520"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /gene="lipB"
                     /locus_tag="VE646_06090"
     CDS             complement(<1..521)
                     /gene="lipB"
                     /locus_tag="VE646_06090"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HZD79592.1"
                     /translation="MTSDGWLLRPLGPVPYERGNAAMHALAERRLAGEIPDTLILLEH
                     PPVYTAGRRWKPDHIVWTEERIRGAGAELHFIDRGGSLTFHGPGQLVGYPIVDLGTKP
                     DAVGYVRRLEDVVIRAAADLGVELHRSDVQTGVWSGRRKVCAIGVRLMRARVTLHGFA
                     LNCTTDLSWYQAIV"
     gene            complement(526..2157)
                     /locus_tag="VE646_06095"
     CDS             complement(526..2157)
                     /locus_tag="VE646_06095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HZD79593.1"
                     /translation="MTTVAMPQLGETVVEGTILKWLKQEGDNVGQDEPLFEISTDKVD
                     TEVPSPTAGVLTKILVPEGQTVKVGTPLAEIGGAGAGAGTDQPQAAQAEQAPPAQAEQ
                     APPAEQPAPQSAEAATHTPPPAEAPAGQAPGTPVQSSAPVETPQVEPAAEAPAAAPAP
                     PQEPAPQPQVAAPSTRAETAAELPDRGARSQILSPLVRRLAAENDVDLSAIVGSGTGG
                     RITKDDVLSYVEAARAGLAQPAAAPPAATVPAQAPGQAPAAQPAPAPPQPAQAPGPTQ
                     APVQPQPAQAPAQPQPAQAPVLPQPAQAPVAPPGAGEEMIPVSHIRRAIGEHMTRSLQ
                     VSARAWNAVEVDVENVAQLRQRAREAFRAREGFNLTYMPFVARAVVDALMAFPLVNAE
                     LRGDQIVLKHFVNLGIAVSYEEGLIVPVVRGADGMNLIGLSRAINDVATRARAKQLKP
                     DEVHGGTHTITNPGPFGSLISVPIINQPETAILAFDTVQKRPVVVDDAIAIRHMVYLS
                     LSWDHRVIDGALASQFLSRVKQNLETWDLGEDLGL"
     gene            complement(2171..3217)
                     /locus_tag="VE646_06100"
     CDS             complement(2171..3217)
                     /locus_tag="VE646_06100"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZD79594.1"
                     /translation="MAQARVEAGAAGHGAEPRGKRTENGEATYLIAIAEAMWEEMERD
                     DRVYLLGEDIGVYGGAFKVTEGFIERFGRERVMDTPIAEETIVGMAMGSAMEGFRPIA
                     EFQYADFMTSGFDEIVTALARYHYRTGVPLPVVLRAPSGGGVRASNFHSTNPEPWFAY
                     APGLKVICPAFPSDAKGLLKSAIRDDNPAVFLEHKWIYRRIREQVPEDPDFLVPIGKA
                     KVQREGTDVSIITYGAMVHQALEAGEELAQQGISAEIVDLRTVYPLDEQTVLASVAKT
                     SRALVLYESSRFLGTGAEVAAMIAERAFTDLDAPVMRLAPPHTPVPFSPVLEDAYIPQ
                     VADIEAAVKELSTW"
     gene            complement(3217..4281)
                     /locus_tag="VE646_06105"
     CDS             complement(3217..4281)
                     /locus_tag="VE646_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HZD79595.1"
                     /translation="MATDVSQMAGGQEADWRVGLSDDQLKDILHKLKLCRYFDERMEA
                     LYRQGRLPGAIYSGRGQEGTHVGVTYPLRKDDSLFPTHRDLSAQLTKGLDLKRVMAQF
                     WGRIDGYTRGRDGNSHIGDWEGTRTFTVISHLPIAYPIATGAALAYLRRGEPRVALAI
                     CGDGSTSNGRWHESLNASAVHQLPVVWVVNNNQYAYSTPNRLEFRVPTIAERAAAYGM
                     PGVRVDGQDVLEVYRVAREAVERARTGEGPSLIESVSLRWRGHAGHDPAKYVPKELLD
                     QYMQRKDPVKRYEDLLVSREVVDEEEIKRVQERVEQEFEEGYEFAQRSPFPVPEDVTK
                     GVFTDDGYWQTEPSREGGTG"
     gene            complement(4294..5673)
                     /gene="lpdA"
                     /locus_tag="VE646_06110"
     CDS             complement(4294..5673)
                     /gene="lpdA"
                     /locus_tag="VE646_06110"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HZD79596.1"
                     /translation="MPDAYDVVVIGAGTGGYACALRAAQLGKRVALIDREERLGGTCL
                     LRGCIPTKALLQSAAVMETIRRSEEWGVHASGEPDWDAVKAFEAKTVDKLVSGLTGVI
                     RSRKIEVIRATATVAPGPAAIADGRRIEASELVLATGSAPKLLPGLAVTDRIITSDQA
                     LWYPTIPRSAVVIGAGAVGLEFASLYRSFGADVTLIEALPRIAPLEDEDLSKELGRQF
                     RRRGIQAHAGASVQTIDEVDGGVRVVYEAGGRAEEASADICLVAVGRGPVTTGLGLEA
                     IGVETDRGFVKVDGLLQTTAAHVWAVGDVAATPLQLAHVAFTEGIAVAERLAGIPSPE
                     IDYAGIPRVTYSSPEVASVGITEAQAIERGEELDVERFNLQGVGKANIVGEGGIVKLI
                     ARKGGGPVLGIHMVGPHVTDLISEGMLITNWEAVPAEVAALIHPHPSLSEAVGEAFLA
                     LAGKPLHSA"
     gene            complement(5761..6471)
                     /locus_tag="VE646_06115"
     CDS             complement(5761..6471)
                     /locus_tag="VE646_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79597.1"
                     /translation="MHKFIGCLVVVLVAAGCRDAAIGSATPPAAPQIHITESPSPFSE
                     VTAGQVRAMIPDTWRPQLAGTLMDAEHGIIASPRPREWRSSTSPIEGMAAMWVDGTRV
                     GVPSDYYYLAATGPALDGLTHRPECSATSRTVFADHRPAFADGPRDSPGDYVAAGQGI
                     CKLGPRPTRWAYFVAAPGFGPMRAIGIPSSGLYVVVAVMHDSPRASATLNKLLQRTRF
                     GSATVADLIAAASGRALS"
     gene            complement(6591..>7029)
                     /locus_tag="VE646_06120"
     CDS             complement(6591..>7029)
                     /locus_tag="VE646_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD79598.1"
                     /translation="TRAIREISPQTRVILFTVDESRTSISEAIQAGVSGYLLKDASAE
                     ELVNAARLALEGKAVIHPQLTRAFIEEVQLAEKRPDVAPLSKREKEILQKVAYGATTK
                     EVAHDLGISPHTVKTHLERIFEKLGANDRAQAVAIAIRTGLVE"
BASE COUNT          981 a         2461 c         2480 g         1107 t
ORIGIN      
        1 acgatggcct ggtaccagga caggtcggtc gtgcagttca aggcgaaccc gtggagggtc
       61 acccgggccc gcatcagtcg cacgccgatc gcgcacacct tccgacggcc ggaccaaacc
      121 cccgtttgga catcgctgcg gtgcagctcc accccgaggt cggcggcggc acggatgacc
      181 acgtcctcga gccggcggac gtacccgacc gcgtccggct tggtgcccag atcgacgatg
      241 gggtagccca cgagctgccc gggtccgtgg aaggtgagcg atcccccccg gtcgatgaaa
      301 tggagctcgg ctccggctcc gcggatccgc tcctcggtcc acacgatgtg atccggcttc
      361 cagcgccgac cggccgtgta cacgggggga tgttcgagca ggatcagcgt gtcggggatc
      421 tcgccggcca accggcgctc ggcgagggcg tgcatggctg cgttcccgcg ctcgtacggc
      481 accggcccga gcgggcggag cagccatccg tcggacgtca tcgcgctaca gcccgaggtc
      541 ctcgcccaga tcccaggtct ccaggttctg cttcacgcgg gagaggaact gggacgccag
      601 agcgccgtcg atcacgcggt ggtcccagga gagcgagaga tagaccatat ggcggatcgc
      661 gatcgcgtcg tccaccacca ccggccgctt ctgcaccgta tcgaacgcca ggatcgcggt
      721 ctcgggctgg ttgatgatcg gcacggagat cagcgagccg aacgggcctg gattggtgat
      781 ggtgtgggtc ccgccgtgga cctcgtcggg cttgagctgc ttggcccgag cccgggttgc
      841 gacgtcgttg atcgccctgg acaggccgat gaggttcatg ccgtcggcgc ctcggaccac
      901 ggggacgatc agcccctcct cgtaggacac ggcgatgccg aggttcacga agtgcttgag
      961 gacgatctga tctccgcgca gctcggcgtt caccagcggg aaggccatga gggcatcgac
     1021 caccgcccgg gccacgaacg gcatgtaggt caggttgaag ccttcccgag cccggaacgc
     1081 ttcgcgggcc cgctgacgca gctgggccac gttctccacg tccacctcga ccgcgttcca
     1141 tgcccgagcc gaaacctgca ggctgcgggt catgtgctcg ccgatggccc ggcggatgtg
     1201 ggagacgggg atcatctcct ccccggcgcc cggaggcgcg accggggcct gagccggctg
     1261 gggtaggacc ggggcctgag ccggctgggg ttgggccggg gcttgggccg gctggggttg
     1321 gaccggggcc tgggtcggcc cgggggcctg ggccggctgg ggcggcgccg gggcgggctg
     1381 cgcggcaggt gcctgaccgg gtgcctgagc cggtactgtc gctgccggcg gggcggctgc
     1441 cggctgcgcc agaccggccc gagctgcttc gacgtagctc aacacgtcgt ccttggtgat
     1501 cctccccccg gtgccgctgc cgacgatcgc ggagaggtcc acatcgttct cggccgccag
     1561 ccgccgcacc agcggagaca ggatctggga gcgtgcccct cgatccggca gctcggcggc
     1621 ggtctccgct cgggtggagg gggcggcgac ctggggttgg ggtgccggct cctgaggggg
     1681 cgcaggggcg gctgccggcg cctccgctgc cggctccacc tggggggtct ccaccggggc
     1741 gctcgactgc acaggggtcc ccggcgcctg ccccgcaggc gcctcggccg gtgggggggt
     1801 gtgcgtggcg gcctcggccg actgaggagc gggctgctcg gccggcggtg cctgctcggc
     1861 ctgagcgggc ggtgcctgct ccgcctgagc cgcttgtggc tggtcggtcc ccgccccagc
     1921 cccggcgccg ccgatctcgg ccaagggcgt gccgaccttc acggtctgtc cctcgggaac
     1981 gaggatcttc gtgaggaccc ctgcggtggg ggaggggacc tccgtgtcga ccttgtccgt
     2041 cgagatctcg aacaggggct cgtcctggcc gacgttgtcg ccttcctgct tcaaccactt
     2101 gaggatggtc ccctcgacga cggtctcgcc gagctgcggc atggctacgg tggtcatctg
     2161 tcctccccgc tcaccaggtg gaaagctcct tgacggcggc ctcgatatcg gcaacctgtg
     2221 ggatgtacgc gtcctccagc acgggggaga agggcacggg cgtgtgcgga ggcgccagac
     2281 gcatcaccgg ggcgtcgagg tcggtgaacg cccgctccgc gatcatcgcc gcgacctcgg
     2341 cccccgtgcc gaggaaccgg ctgctctcgt acagcaccag ggctcggctc gtcttggcca
     2401 cggacgccag cacggtctgc tcgtccaggg ggtacacggt ccgaaggtcc acgatctccg
     2461 ccgagatgcc ctgctgcgcg agctcctccc cggcctccag ggcctggtgg accatcgcgc
     2521 cgtaggtgat gatcgagacg tcggttccct cccgctgcac cttcgccttg ccgatcggga
     2581 ccaggaagtc cgggtcttcc gggacctgct cgcggatgcg ccggtagatc catttgtgct
     2641 ccaggaagac cgccgggttg tcgtcacgga tggcggactt cagcagccct ttggcatccg
     2701 aggggaaggc cggacagatc accttcaggc ccggcgcgta cgcgaaccac ggctcgggat
     2761 tggtggagtg gaagttgctg gcgcggaccc cgcctccgga gggcgcgcgc aggaccacgg
     2821 gcagcggcac gccggtccgg tagtggtacc gggccagggc ggtgacgatc tcgtcgaacc
     2881 cggaggtcat gaagtcggcg tactggaact cggcgatggg tcggaagccc tccatcgccg
     2941 agcccatggc catgccgacg atcgtctcct cggcgatcgg cgtgtccatg acgcgctcgc
     3001 gaccgaagcg ctcgatgaac ccctcggtga ccttgaaggc gcctccatag accccgatgt
     3061 cctcgccgag caggtagacg cggtcgtcgc gctccatctc ctcccacatc gcttcggcga
     3121 tcgcgatcag gtacgtggcc tccccgttct cggtacgctt gcctcgcggc tcggccccgt
     3181 ggccggcggc accggcctca acgcgcgctt gggccatcag ccggttcccc cctcgcgcga
     3241 gggttcggtc tgccagtacc cgtcgtccgt gaagaccccc ttggtcacgt cttcggggac
     3301 cgggaagggc gagcgctgtg cgaactcgta tccctcctcg aactcctgct ccacccgctc
     3361 ctgcacgcgc ttgatctcct cctcgtcgac gacctcgcgg ctgacgagga ggtcctcgta
     3421 ccgcttcacg ggatccttgc gctgcatgta ctggtcgagc agctccttcg gcacgtactt
     3481 ggccgggtcg tggccggcgt gcccccgcca ccgcagcgag accgactcga tcagcgaggg
     3541 tccctccccc gtgcgagccc gctccaccgc ctcacgggcc acccggtaga cctcgagcac
     3601 gtcctgtccg tccacgcgga ccccgggcat cccgtacgcg gccgctcgct cggcgatggt
     3661 ggggacccgg aactccagcc gattgggggt cgagtacgcg tactggttgt tgttcaccac
     3721 ccagaccacc gggagctgat ggacggccga ggcgttcagc gactcgtgcc agcgcccgtt
     3781 cgaggtggac ccgtccccgc agatcgccag ggccacccgc ggctctccgc gccgtaagta
     3841 ggcgagcgcc gcgcccgtgg cgatggggta cgcgatcggc aggtgcgaga tcacggtgaa
     3901 ggtccgcgtg ccctcccagt cgccgatgtg gctgttcccg tcgcgacccc gcgtgtagcc
     3961 gtcgatccga ccccagaact gagccatgac ccgcttgagg tcgagcccct tggtgagctg
     4021 ggccgacagg tcccgatggg tggggaacag cgaatcgtcc ttgcgcaacg ggtaggtcac
     4081 gccgacgtgg gtcccctcct gtccgcgacc cgagtagatg gcccccggga gcctgccctg
     4141 ccggtacagc gcctccatcc gctcgtcgaa gtagcggcag agcttgagct tgtgcaggat
     4201 gtccttgagc tgatcgtcgg agagaccgac ccgccagtcg gcctcctgac cccccgccat
     4261 ctgcgatacg tcggtcgcca aagcgcggtc cccctacgcg gaatgcagcg gcttgccggc
     4321 gagggccagg aaggcctcgc ccaccgcctc ggacaaggac ggatggggat ggatcagggc
     4381 ggccacctcg gccgggaccg cctcccagtt ggtgatcaac atgccctcgg agatcagatc
     4441 ggtgacgtgc ggccccacca tgtggatccc cagcaccggg ccgcctccct tccgggcgat
     4501 gagcttcacg atgcctcctt cgcccacgat gttggccttc cccacgccct gcaggttgaa
     4561 ccgctccaca tcgagctctt caccccgctc gatggcctgg gcctcggtga tccccactga
     4621 ggcgacctcg ggagaggagt acgtgacgcg cgggatcccc gcgtagtcga tctcggggga
     4681 agggatgccg gcgaggcgtt ccgccaccgc gatgccctcc gtgaacgcca cgtgcgcgag
     4741 ctgcaggggg gtggccgcca cgtcgccgac cgcccacacg tgggcggcgg tggtctggag
     4801 gagaccgtcg accttcacga agccgcggtc ggtctccacg ccgatcgcct cgagccccag
     4861 accggtcgtg accggacctc ggcccacggc caccaggcag atgtcggcgg acgcctcctc
     4921 ggcgcgtccg cccgcctcgt agaccacccg gacgccaccg tcgacctcgt cgatggtctg
     4981 cacgctcgct ccggcgtggg cttggatccc ccggcgtcgg aactggcgcc cgagctcctt
     5041 ggagaggtcc tcgtcctcca ggggcgcgat ccgggggagg gcttcgatca gggtcacatc
     5101 ggcgccgaag gaccggtaca gggacgcgaa ctcgagcccc acggcgccgg cgccgatcac
     5161 cacggccgag cgtgggatcg tcgggtacca cagggcctga tcgctggtga tgatccggtc
     5221 ggtgaccgca agcccgggca ggagcttggg ggccgacccc gtcgcgagca ccagctcgct
     5281 cgcctcgatc cgacggccgt ccgcgatggc tgccggaccg ggggcgaccg tggccgtcgc
     5341 gcggatgacc tcgatcttcc gggaccggat gacgccggtg agacccgaga ccagcttgtc
     5401 caccgtcttg gcctcgaagg ccttgaccgc gtcccagtcg ggctcccccg aggcatgcac
     5461 gccccactcc tccgacctac ggatcgtttc catgaccgca gcggactgga gcagcgcctt
     5521 ggtggggatg catccgcgca gcaggcaggt gccgcccagt cgttcttccc gatcgatcaa
     5581 cgccacgcgc ttgcccagtt gcgcggcccg cagggcacag gcgtagccgc ccgtacccgc
     5641 accgatcacg acgacgtcat aggcatccgg catgcgaacc tccatgttac cgccggtggt
     5701 ggaacgaccg ggtctaggat cgggcccgcc ggcttgatga tgtggggacg gcggggatgc
     5761 tcaggacagc gctcggcccg acgccgcagc gatcaggtcg gcgaccgtcg cgcttccgaa
     5821 gcgcgtgcgc tgcagcagct tgttcagcgt cgcgctggcc cgtggcgagt cgtgcatcac
     5881 cgccaccacc acgtacaggc cggagctcgg gatcccgatg gctcgcatcg gtccgaagcc
     5941 gggcgcggcc acgaagtacg cccaccgcgt gggtctgggt cccagcttgc agatgccctg
     6001 ccccgctgcg acgtagtctc ccggggagtc cctgggcccg tcggcgaagg ccggccgatg
     6061 atccgcgaac accgtccggc tggtggcgct gcactcggga cgatgggtga gcccatcgag
     6121 cgccgggccg gtcgccgcca ggtagtagta gtcggagggc acccccaccc tggtcccgtc
     6181 cacccacatc gccgccatgc cctcgatggg ggatgtggag gatcgccact ccctcgggcg
     6241 gggagaggcg atgatcccgt gctccgcgtc catgagggtt ccggcgagct gaggccgcca
     6301 ggtgtcgggg atcatggccc ggacctggcc cgcggtgacc tcgctgaagg ggctggggga
     6361 ttcggtgatg tggatctggg gagcggccgg aggcgtcgct gacccgatgg ccgcatcgcg
     6421 gcagcccgcg gcgaccagga cgacaaccag gcagccgatg aacttgtgca cccgccgatc
     6481 ggttcgagca tagtcgccgg ggggccggcc cgccagtcgg cgggcgggcc tacggggggg
     6541 cggggcgagg ggtggaggac cgctcctcct cccggacgcc cggcctccag ctactcgacc
     6601 aggccggtgc ggatcgcgat ggcgaccgcc tgggcgcgat cgttcgcgcc gagcttctcg
     6661 aagatgcgtt cgaggtgggt cttcacggtg tggggcgaga tcccgaggtc gtgagccacc
     6721 tccttggtgg tggcgccgta ggccaccttc tggaggatct ccttctcgcg cttggacagg
     6781 ggagccacat cgggccgctt ctcggcgagt tgcacctcct cgatgaaggc gcgggtgagc
     6841 tgtgggtgga tcacggcctt gccctcgagc gccagccggg ccgcgttcac cagctcttcg
     6901 gccgaggcgt ccttcagcag gtacccggag acgccggcct ggatggcctc cgagatggac
     6961 gtgcgggact cgtccaccgt gaacaggatc acccgggtct gcggcgagat ctcccggatg
     7021 gcgcgggtt
//
LOCUS       DASQQQ010000149         8790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_260680, whole genome shotgun sequence.
ACCESSION   DASQQQ010000149 DASQQQ010000000
VERSION     DASQQQ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8790
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260680"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VE646_06125"
     CDS             <1..547
                     /locus_tag="VE646_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HZD79599.1"
                     /translation="LASSEQDLSADPHVWLDPVLMQQIVHRIGDALARADTAHASADR
                     QREGRTVRVLQRLDAAYRGGLASCRFTTFVTSHEAFGYLAQRYGLHQLGIEGLAPESE
                     PSAQRIQAAATAIQQGRAAPAVFYEETAEGRRVGQSVAADVGVPALPLGTVATVPTPG
                     DYLSVMRENLSSLEQGLRCAS"
     gene            535..1272
                     /locus_tag="VE646_06130"
     CDS             535..1272
                     /locus_tag="VE646_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HZD79600.1"
                     /translation="MRELMEVQAVRDAVELAEVRFGYGAAPVLDGVNLRLRSGQIAVL
                     AGANGSGKSTLLRLLVGLLRPDAGIVRVLGGSPVDPAIRRRIGYAPQGLRTVTSLPVS
                     VAEVVAAGITPSKGVFARMGRSDWRRVQASIEAVGLEGLSRECLFELSGGQQQRAIIA
                     RALVGDPDLLLLDEPTTGIDPRSRPIVVREFRRRAEAGATVIAVSHDPDEFHEVCDRI
                     LVLESGRLEPVTHDEFHHRFHQHAGPT"
     gene            1269..2099
                     /locus_tag="VE646_06135"
     CDS             1269..2099
                     /locus_tag="VE646_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HZD79601.1"
                     /translation="MSRLFLVWAVQAYLAGAAVGLAAPVIGCFLVQRRLSLIGDAVGH
                     LAFAGVGLGVAVGLAPLWGALAIAVLGAVGLEWLRVRGRLAGDLSLALIFYLGLALGL
                     VIISARGGLTASVQGVLFGSIFTITWSDVLAIVVLCAVVVAFVSVFYKELVAVALDEE
                     TARASGLQVDRLNVLLVVLTALLVAAGMRVVGLLLIASLLVVPVAAGSRLAHSFRGSL
                     LWAAAAGVASTIAGLVLALVQGAISPGGTIVLTSAVLFVLASAVGGRAARRHPRMVAP
                     "
     gene            2096..2596
                     /locus_tag="VE646_06140"
     CDS             2096..2596
                     /locus_tag="VE646_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HZD79602.1"
                     /translation="MSDRRQMWAAAGLRTTRQRETIVGALEEAGRPITAQALHARLSR
                     AGGRAPGLATVYRTLQALEAAGYARTFPGAEGEASYKLCDPGHHHHLICEGCGRVVEI
                     PSCEIEGWAKTAARRRGFSVSSHQADIFGLCPPCRRTAAGVAAAPVRASGRAERAGQR
                     PGSRTR"
     gene            complement(2538..3206)
                     /locus_tag="VE646_06145"
     CDS             complement(2538..3206)
                     /locus_tag="VE646_06145"
                     /inference="COORDINATES: protein motif:HMM:NF044818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79603.1"
                     /translation="MVLGAWALLFWFLWLSGRDALYLSSRTSWVVPVGAILLTAASSG
                     RLAAARVRLPEPVGRREAWILGAMVLPVVLVAALPTATLGSFSAGKRTGFTGAGLATP
                     LGDIGTGQLTLVDVAAAETSGPGEAALAKRAGEQVSFTGFVTRYADTPADEFELSRYI
                     VTCCVADATIAQVRVVNVPPGVFDPDQWVEVSGTIYPLGSEVIISASSIRGVARPSPP
                     YLTP"
     gene            complement(3302..4261)
                     /locus_tag="VE646_06150"
     CDS             complement(3302..4261)
                     /locus_tag="VE646_06150"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HZD79604.1"
                     /translation="MAVVAALAAITVLLRLAAPERVAWIRTAFLIFASFLVQALPFVL
                     IGALAAAVIEVFVPLGTLEKLARLPRPLQLPAAGLAGLAFPICECGSVPVARRLAAKG
                     MEPSAAVTFMLAAPVVNPVVIASTFVAYHGRSMQWTMVAGRFLLGLLVAVAVGWVVGD
                     RSREELLRPRADEGHAHHVLELGPPEARWRRFFTHLGNDFLYMGRFLLLGATIAAMVQ
                     TFLPASVIGSVAGIPVLNMVVMMGLASVLSLCSESDAFIAASFVQFGPAAQLAFLVFG
                     PMVDLKLGALYMGTFRRGFFRMLVIAATATTLVGALWIGVIAG"
     gene            complement(4315..5637)
                     /locus_tag="VE646_06155"
     CDS             complement(4315..5637)
                     /locus_tag="VE646_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79605.1"
                     /translation="MFGRRLLVLLVILGALAIPAGALRATCAGRSCSGGSDAPARIPF
                     CSLPSAIKADLVAGFRQGRSPDILAVAAHALSGGTGQTGAQAPWPALTPGPDTSVPLV
                     FAGRGVDPAARVPRGTGLDQVAPTVARILGFRRPHPEIPAGTAVPGVPDGTRPRLVLE
                     IAWKGAGGAELRRAPQAWPTLASLLRRGTGTLSGTTGSLPLDPTATLTTIGTGGPPSQ
                     HGITGTLLRNRDGHLVHAWGPGSPPSVIPTLADDVGRASDRAAMIGLVAATRSDRGIV
                     GGTWYRGQDRDPVRLALGGAPVSRTRRLLATGFGRDGVPDILAVVLDGSIRSMDRRTR
                     RVIAAARQAAARSLLVVVAGTGSSAADRGIGADEVAARVEAGVPGGVDVIAGTVAGGI
                     FLDQDALATLRITGQTVTKAVLDARAPDGSPLFADAFQGFAVSFARYC"
     gene            5961..7520
                     /locus_tag="VE646_06160"
     CDS             5961..7520
                     /locus_tag="VE646_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZD79606.1"
                     /translation="MAPGSLEEVACSLPSDWLLRTWRGYEADRGPEIQILPKEYNFVG
                     SGLPHVGPWDFVNDVPMFWYGPGHIRAQGAVDRPVTSAGIAPTQAKLLDFDRYQAPDG
                     IPMSEAIAPGQQPPKLIVTMVWDAGGMNVLDRWKDAWPYLRSLIPEGTWYTNASVGSS
                     PTSTAQIHATIGTGAFPDHHEMIGHRFRIGDHITTPWAQGPAYLMEPTLADLYDRAMG
                     NRPVVGEQGTVSIHLGMLGHGAMWGGGDKDVAVTREAVGATTLGAETFRWNLSPLLSQ
                     SFRFPSYVNDVPGFRQDVRAVDAADGQIDGKWRTNDIAQLLNGFDTPARTPYQTRVLE
                     EMVRREGFGADDLPDLLFVNYKEIDYISHVWSVDSLEMRDAVVAQDVALKEFVSFLDR
                     QVGRGDWVLLVTADHGSMIPTRISGGFQISSTPIAAGINARFDHDGDDTHIVELIQPT
                     QIFINTKELRQNGYTLEDVSRYLMDLTRAQTAGAGVAVDPSTADQTVFQAAFPSSIMK
                     GLPCLPETHGG"
     gene            7537..>8790
                     /locus_tag="VE646_06165"
     CDS             7537..>8790
                     /locus_tag="VE646_06165"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZD79607.1"
                     /translation="MRLWPALLALAFVASACTSSGVESGTPSAASPAGSVPPSAPPPP
                     ISNLAMAQRVCAAVSHEVLLRTWRGYFPGRGGDVQIIPTYPNFVNGGLTHATPYAYTQ
                     EVPLFLYGPGYVRPGVYPRRATLADIAPTAGALLKFPFHAPDGTAQQQALLPADQRPL
                     PKLLVTMVWDSAGWDVLDRWKDDWPYLRSLLPEGALFTDVTVGSSPSNTPTGHSIIGT
                     GAFPRRTGFVDEYIRVNGRIQKPNAEGPAFLLQPTLADLYDRAMGNRPIVGGIASLSA
                     HITMMGHGSLWGGADRDLAVTREAQFGPTGGAESDVWNLTDAMAPFYTFPGYVNSLPP
                     VTAYTRSLDQADGQLDDAWRGHPFTRLHNGFDTPARSPYQEALIETMVQREGFGRDDV
                     PDLLDLNYKAIDTIGHLFSADGVEMS"
BASE COUNT         1253 a         3113 c         3189 g         1235 t
ORIGIN      
        1 gctggcttcc tccgagcagg atctgtcggc cgatccgcac gtctggctgg accccgtgct
       61 gatgcagcag atcgtccatc gcatcgggga tgcgctcgcc cgggccgaca cggcccacgc
      121 ttcggccgac cggcagcgcg agggtcggac cgtgcgggtc ctgcagaggc tcgacgcggc
      181 ctaccgagga gggctggcga gctgccggtt caccacgttc gtgacctccc acgaggcgtt
      241 cgggtatctg gcccagcggt acgggctcca ccagctcggg atcgaggggc tcgcgcccga
      301 gagcgaaccc tccgcccaac ggatccaggc ggcggcaacc gcgatccagc agggtcgggc
      361 ggccccggcc gtgttctacg aggagacggc tgagggccgc agggtggggc agagcgtggc
      421 cgcggacgtg ggggttccgg cgctgccgct cgggacggtg gcaacggtac cgacaccggg
      481 cgactatctg tcggtgatgc gagagaacct gtccagcttg gagcaggggt tgcggtgcgc
      541 gagctgatgg aggtgcaggc ggtccgggat gcggtggagc tcgccgaggt gcggttcggc
      601 tacggggccg ctcccgtcct ggacggcgtg aaccttcggc tgcgctcggg ccagatcgcc
      661 gtcctggccg gggcgaacgg cagcgggaag tccacgctcc tgaggctcct cgttggcctg
      721 ctccggccgg atgccgggat cgtccgggtg ctgggcggat cgccggtgga cccagccatc
      781 aggcggcgga tcgggtacgc gcctcagggg ttgcgcaccg tcacgtcgct cccggtgtcc
      841 gtggccgagg tggtggcggc gggcatcacg ccctccaagg gcgtgttcgc gcggatgggc
      901 cgttcggact ggcgccgggt ccaggcctcg atcgaggcgg tcggactgga ggggctgtcc
      961 cgggagtgcc tgttcgagct ctccggaggc cagcagcagc gggccatcat cgctcgagcg
     1021 ttggtcggcg atcccgacct gctcctcctg gacgagccga ccaccggaat cgatccgcga
     1081 tcccggccca tcgtggtccg cgagttccgc cggcgggccg aggcgggggc caccgtgatc
     1141 gccgtctccc acgaccccga tgagttccac gaggtgtgcg accggatcct ggtgctcgag
     1201 agcgggcggc tggagccggt cacccacgac gagttccatc accgcttcca ccagcacgcg
     1261 gggcccacgt gagccggctg ttcctggtgt gggcggtcca ggcgtacctc gccggggcgg
     1321 cggtcgggct ggccgcgccc gtgatcggct gcttcctggt ccagcgacgg ctgtcgttga
     1381 tcggcgacgc cgtgggacac ctggccttcg ccggggtggg cctgggggtg gcggtcgggc
     1441 tggcgcccct gtggggcgcc ctggcgatcg ccgtactggg cgccgtcggg ctggagtggc
     1501 tgcgggttcg gggtcgcctg gccggggacc tgtcgctcgc gctcatcttc tacctggggc
     1561 tggcgctcgg actcgtgatc atctcggccc ggggcggtct cacggcctcg gtgcaaggcg
     1621 tgttgttcgg ctcgatcttc acgatcacat ggagcgatgt gctcgcgatc gtggtgctgt
     1681 gtgccgtggt ggtggcgttc gtttccgtct tctacaagga gctggtggcc gtcgccctgg
     1741 atgaggaaac ggcccgagcc tcggggcttc aggtggatcg gttgaacgtc ttgttggtgg
     1801 ttctgacggc cctgctggtg gcggccggga tgcgggtggt ggggctgctg ctgatcgctt
     1861 cgttgctggt ggtcccggtg gccgcgggct cccgcctggc ccactcgttc cgggggtccc
     1921 tgctgtgggc cgccgcagcc ggcgtggctt ccaccatcgc cggtctggtc ctcgccctgg
     1981 tccagggggc catctcgccc ggcggcacca tcgtgctcac ctcggccgtc ctgttcgtgc
     2041 tggcatcggc ggtgggcggg cgggcggccc gccgtcaccc gcggatggtg gccccgtgag
     2101 cgatcgtcgg cagatgtggg ccgcggcggg gctccgcacg acacgacagc gggagaccat
     2161 cgtgggcgcg ctggaggagg cggggcgacc gatcacggcc caggccttgc acgctcggtt
     2221 gtcccgtgca ggcgggagag cgccgggcct ggccaccgtc taccggaccc tccaggcgct
     2281 cgaggcggcc ggctacgcga ggaccttccc cggcgccgag ggggaagctt cgtacaagct
     2341 gtgcgatccc gggcaccacc accacctgat ctgcgagggt tgcggtcggg tggtcgagat
     2401 cccttcctgc gagatcgagg ggtgggcgaa gaccgcggct cggcgcaggg gattcagcgt
     2461 ctcctcccac caggcggaca tcttcggtct gtgcccgccg tgccgccgga ccgcggccgg
     2521 ggtggctgcc gccccggtca gggcgtcagg tagggcggag agggccgggc aacgccccgg
     2581 atcgaggacg cgctgatgat gacctcgctg ccaagcgggt agatggtgcc gctcacctcg
     2641 acccactgat cggggtcgaa gacgccgggc ggcacgttca ccacgcggac ctgcgcgatg
     2701 gtggcgtcgg ccacgcagca cgtcacgatg taccggctga gctcgaactc gtcggccggt
     2761 gtgtccgcgt accgcgtgac gaagcccgtg aagctcacct gttcgccggc gcgtttggcc
     2821 agggcggcct cacccggccc ggaggtctcg gcggcggcca cgtcgaccag ggtcagctgc
     2881 ccggttccga tatcgcccag gggcgtggcc agccccgcgc ccgtgaaccc ggtgcgcttg
     2941 ccggcggaga agctgccgag ggtggcggtc gggagcgcgg ccaccaagac gacgggcagc
     3001 accatcgctc cgaggatcca ggcctcccgc cggccgaccg gctcggggag ccggacccgt
     3061 gcagccgcca agcgtcccga ggaggcggcg gtgagcagga tcgcacccac gggcaccacc
     3121 caactggtcc gtgaggacag gtagagggca tcgcgccccg agagccagag gaaccagaac
     3181 aacagcgccc acgcgccgag caccacccct ccggcgatcc tcgatgcgct ccaggccgtg
     3241 ggcgctcggg cggagcgcgt tctgagcgcc ggcgcggcgg gtcgcggaag gaggacgtcg
     3301 ctcatcccgc gatcaccccg atccacagcg caccgaccag cgtggtggcc gtggcagcga
     3361 tcacgagcat gcggaagaag cccctgcgga aggtccccat gtagagggcg ccgagcttga
     3421 ggtccaccat cggaccgaag accaggaagg cgagctgggc cgccgggccg aactgaacga
     3481 aggaggcagc gatgaacgcg tcggactcgc tgcataagga cagcacggat gccagcccca
     3541 tcatcaccac catgttcagc accgggatgc ccgccacgct cccgatcacc gaggccggca
     3601 ggaaggtctg caccatggcg gcgatcgtgg cgccgagcag gaggaaccgg cccatgtaca
     3661 ggaagtcgtt gcccaggtgc gtgaagaacc ggcgccaccg cgcctcgggc gggccgagct
     3721 ccaggacgtg gtgcgcgtgt ccctcatcgg cgcgtggccg caggagctct tccctggacc
     3781 ggtcgcccac gacccagccc accgcgacgg ccaccagcag gccgagcagg aaccggcccg
     3841 ccaccatcgt ccactgcatg ctccgtccgt ggtaggccac gaacgtcgat gcgatcacca
     3901 ccgggttgac cacgggcgcg gccagcatga acgtcaccgc ggccgagggc tccatcccct
     3961 tggccgccag acgcctggcc acgggcaccg agccgcactc gcagatgggg aaggccagcc
     4021 cggccagacc ggcggcgggg agctgcagag gccgggggag ccgggccagc ttctccagcg
     4081 tcccgagggg cacgaacacc tcgatcacgg ctgcagcgag ggccccgatc aggacgaacg
     4141 gcagggcctg gaccaggaag gaggcgaaga tcaggaaggc ggtcctgatc caggccacgc
     4201 gctcgggagc ggccagccgg agcagcacgg tgatcgcggc cagagccgcg accacagcca
     4261 tgagggtgcg aggggacgcg gtccggccac gggtcccccc ggcgatcgcg accatcagca
     4321 gtacctcgcg aaggacacgg cgaagccctg gaacgcatcg gcgaagaggg gggatccgtc
     4381 gggagcccgg gcgtcgagca ccgccttggt caccgtctgc ccggtgatcc gcagggtggc
     4441 caacgcgtcc tgatcgagga agatgcctcc cgcgacggtc ccagcgatga cgtcgacccc
     4501 gccggggacg ccggcctcga cacgggcggc gacctcgtcc gctccgatcc ctcggtcggc
     4561 cgccgaggag ccggtcccgg caaccaccac cagcagcgac cgcgccgccg cctgccgggc
     4621 cgccgcgatc acgcggcgcg tgcgccgatc catggagcgg atcgagccgt cgaggaccac
     4681 cgccaggatg tcgggtacac cgtcgcggcc gaacccggtg gccaggagac gccgggtccg
     4741 gctcaccggg gcaccgccca gcgcgagccg taccgggtcc cggtcctgcc cccggtacca
     4801 ggtgccgccc acgatgccac ggtccgatcg cgtggcggcg accagcccga tcatcgccgc
     4861 tcgatcgctc gctcgaccga cgtcgtcggc caatgtcggg atcaccgagg gcggtgagcc
     4921 cgggccccag gcatggacga ggtgcccgtc gcggttccgc aggagggtcc cggtgatccc
     4981 atgctgggag ggcggtcctc cggtaccgat cgtggtcagc gtcgccgtcg gatccagcgg
     5041 cagggagccg gtggtccccg acagcgtgcc ggtgccccgc ctgagcaggg aggcgagcgt
     5101 cggccaggct tggggggcgc gacggagctc ggcgcccccc gcgcccttcc aggcgatctc
     5161 gagcaccagc cgcggtcggg tcccatcggg gacgccgggg acggcggtcc cggccgggat
     5221 ctccggatgg gggcgccgga agcccaggat ccgggcaacc gtgggcgcga cctgatccag
     5281 cccggtcccc cgggggacgc gggcggcggg gtccacgccc cgcccggcga agacgagggg
     5341 gaccgacgta tccgggcccg gggtcagggc gggccagggc gcctgcgccc ccgtctgccc
     5401 cgtcccgccg ctcaacgcat gcgcggctac cgccaggatg tccggggacc ggccctgccg
     5461 gaacccggcg accagatcag ccttgatggc cgagggcagg gagcagaagg ggatccgcgc
     5521 cggggcatcc gagccgccgg agcacgagcg ccctgcacac gttgcccgca gggccccggc
     5581 ggggatcgcg agggcgccga ggatcacgag caggacgagg agccgacgcc cgaacacggc
     5641 ctctcacggt accgcgtggg agcctcaccg gctgcggcgc ggtcggtctg ctaggttgcc
     5701 cgtcgcccgt ggtgtggtct cccaagcgct ccgtggctcc ccggcgcctc tcggcgctgg
     5761 tgttcgtgct ggccctcgtg gggggagcct gcggcggcag cgatgggcag ccttcggggg
     5821 cagatgttcg gggcgggaca tcgaccaagg ccgggacgtc ggcctccccc gccgcgccgg
     5881 cgccggcctc ccccgccgct ccggtggctg cgacctcggg tgcgccggcc ccgtcttccg
     5941 accccacgag ctcggccgcc gtggcgccgg gcagcctcga ggaggtcgcc tgctcgctcc
     6001 cgagcgactg gctccttcgg acctggcgcg gctacgaggc cgaccggggt cccgagatcc
     6061 agatcctgcc caaggagtac aacttcgtgg ggtccggtct gccccatgta gggccctggg
     6121 acttcgtgaa cgacgtcccg atgttctggt acgggcccgg ccacatccgg gcccaagggg
     6181 cggtggaccg accggtcacg tcggccggga tcgctcccac ccaggccaag ctgctggact
     6241 tcgatcggta ccaggcgccc gacgggatcc cgatgagcga ggcgatcgcc ccggggcagc
     6301 aacctcccaa gctgatcgtg acgatggtgt gggatgccgg cggcatgaac gtcctggacc
     6361 gatggaagga cgcctggccc tacctccgat cgctgatccc agaagggacc tggtacacga
     6421 acgcctcggt gggctcctcg cccaccagca cggcacagat ccacgccacg atcggcaccg
     6481 gcgccttccc cgaccaccac gagatgatcg gtcaccgctt ccggatcggc gatcacatca
     6541 ccaccccgtg ggcgcagggt cccgcgtacc tgatggagcc caccctggcc gatctgtacg
     6601 accgcgccat gggcaaccgc cccgtggtgg gtgagcaggg cacggtctcg atccacctgg
     6661 ggatgctcgg tcacggggct atgtggggcg gcggggacaa ggacgtcgcc gtgacccggg
     6721 aggcggtggg cgccacgacc ctgggggcgg agacgttccg ctggaacctc agcccgctgc
     6781 tcagccagtc gttccgcttc ccctcctacg tgaacgacgt gccggggttc cgtcaggacg
     6841 ttcgggcggt cgacgccgcg gacgggcaga tcgacggcaa gtggcgcacg aacgacatcg
     6901 cgcagctgct gaacggattc gacaccccgg cccgcacccc gtaccagacc cgggtgctcg
     6961 aggagatggt ccgccgggag gggttcgggg cagatgacct tcccgacctg ctcttcgtca
     7021 actacaagga gatcgactac atcagccacg tgtggagcgt ggactccctc gagatgcggg
     7081 acgccgtggt ggcgcaggac gtcgccctga aggagttcgt ctccttcctc gatcggcagg
     7141 tgggacgggg tgactgggtc ctgctggtga ccgccgatca cggctcgatg atccccaccc
     7201 ggatctccgg cggcttccag atctcctcca ccccgatcgc ggccgggatc aacgccaggt
     7261 tcgatcacga cggagacgac acccatatcg tggagctgat ccagcccacg cagatcttca
     7321 tcaacacgaa ggagctgcgg cagaacgggt acaccctgga ggacgtctcc cgctacctga
     7381 tggatctgac ccgagcccag acggccgggg ccggcgtggc cgtggacccg tccacggcgg
     7441 accagaccgt cttccaggcc gcgttcccct cgtcgatcat gaagggcctc ccctgcctgc
     7501 ccgagacgca tggcgggtga tcggatccac tcgcgcgtga ggctctggcc cgccctcctc
     7561 gcgctggcgt tcgtggcgag cgcttgcacc tcgtccgggg tggagagcgg cacgccttcc
     7621 gcggcatccc cggccgggtc ggtcccaccc tcggccccgc cgccgccgat ctcgaacctg
     7681 gccatggcgc agcgggtgtg tgccgccgtg tcccacgagg tgctcctccg gacctggcgg
     7741 gggtacttcc ccggccgagg cggcgatgtc cagatcatcc cgacctaccc gaacttcgtg
     7801 aacggcggac tgacccatgc caccccgtac gcgtacacgc aggaggtccc gctcttcctg
     7861 tacgggccgg gatacgtgcg cccgggcgtc taccctcggc gggccaccct cgccgacatc
     7921 gctcccaccg ccggcgccct gctcaagttc ccgttccacg cgccggacgg cacggcgcag
     7981 cagcaggcgc tgcttcccgc cgaccagcgc ccgctgccca agctgctggt cacgatggtc
     8041 tgggactccg ccgggtggga tgtcctcgat cggtggaagg acgactggcc gtacctgcgt
     8101 tcgctgcttc ccgagggcgc actgttcacc gacgtgaccg tgggctcctc accctccaac
     8161 accccgaccg ggcactcgat catcgggacg ggggccttcc cccggcgtac ggggttcgtg
     8221 gacgagtaca tccgggtgaa cggccggatc cagaagccga acgccgaggg cccggcgttc
     8281 ctgctccagc ccaccctggc cgacctgtac gaccgcgcca tgggcaaccg cccgatcgta
     8341 ggggggatcg cgagcctgtc ggcccacatc acgatgatgg gccacggctc cctgtggggc
     8401 ggcgccgacc gggacctggc cgtcacgcgc gaggcgcagt tcggccccac cggcggcgcc
     8461 gagtccgacg tgtggaacct gaccgacgcg atggctccct tctacacgtt ccccgggtac
     8521 gtgaactcgc ttccgcccgt caccgcgtac acgcggtcct tggatcaggc ggacggtcaa
     8581 ctcgacgatg cctggcgcgg gcaccccttc acccggctgc acaacggctt cgatactccg
     8641 gcccggagcc cctaccagga ggccctgatc gagaccatgg tccagcggga gggcttcggt
     8701 cgggacgacg tgccggacct gctcgatctg aactacaagg cgatcgacac gatcggtcac
     8761 ctcttcagcg ccgacggcgt ggagatgtcc
//
LOCUS       DASQQQ010000150         6241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_226933, whole genome shotgun sequence.
ACCESSION   DASQQQ010000150 DASQQQ010000000
VERSION     DASQQQ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226933"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VE646_06170"
     CDS             complement(<1..930)
                     /locus_tag="VE646_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZD79608.1"
                     /translation="MNERTVLEFIRQRGPVSRAQIARDSGLSKPTVSQALAALERTGL
                     VREAGRSTGGKGPSALLYELDATAGWVVGIDVGRAWLRAAIANLTGDIVARRDERAKA
                     RSARSLIEQIGRVAHGLAGEAGLRWRQVTFAVVGSPGVVRPARDQVVLAHNLPGWGRH
                     GLIEAVQEELGTKTAFENDVNLATVGEHWKGLGKGVDNFVYLHVGTGVGLGLVVNGEL
                     YRGATGIAGEIAYLPLVADPHHPASRRHGALESVLGAKGLLTFARSRGLRSPLSLRTV
                     FSAARRGDQDAAAVVEVAIRYIALAIATVVPVMD"
     gene            complement(1063..2358)
                     /locus_tag="VE646_06175"
     CDS             complement(1063..2358)
                     /locus_tag="VE646_06175"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 4"
                     /protein_id="HZD79609.1"
                     /translation="MTKICVIGGGSAYMPGIAYAFAKLADHFPDTTVTLHDIDPDALD
                     LQTRLSGSILRSRGVRGVRVDASRDRAGALEGADMVLTAFRPGGFPARQLDESIPPRY
                     GVIGQETAGPGGFAMALRSIPVLLDIAKEVRKVGAPGAVILNYTNPVQVVTDALVRYG
                     DVPFIGLCDQTSGEIGFLARLMGVNRGALEIDTSGTNHMTFTRAVRLDGVDVTGRVWN
                     LLDGLDLGSLATEAERRIVRLFRVLRLIPSEYLQYFFFHDQVLAEQRSKGRTRAGEIM
                     AQLPEVLESYRREADAADPRPSMARASEEHGDFAVEVMAAMLSGRPGRFILNVPNRGA
                     ITDLPADAVVEVPCRVMGRTIQPLAQGAMPAMVAGLVRQVVEHARLAADAAVDGEREA
                     CVRALLAHPLVRSLETAEALTEEFLTAHAAWLPQFARLS"
     gene            2424..3674
                     /locus_tag="VE646_06180"
     CDS             2424..3674
                     /locus_tag="VE646_06180"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZD79610.1"
                     /translation="MAELAINGGSPVRPQGFPAWPEWDEREVRAVTEVVRSGNWGGFP
                     EPGPNASAFEDAFAAYQGAAHGILMVNGTITMEVALKALDIGWGDEVIVPALTFVATA
                     YAPMAAGALPVIVDVDPRTWTIDPDQVEAAITPRTKAIMPVHLGHQMADMDRIMKIAQ
                     QHSLAVVEDCAHAHGQRWRDRGAGCIGDLGSFSHQSSKILTAGEGGSLLTNDSGLASR
                     AHSIIDCGRPKDPEERRYTFGANYRLGELHAALLVTQLERFEDQRRQRVEGAEVFEEL
                     APQVPGVSIMPHDPRVTRWSFYNYVFAIEPEEFAGATNETVAAALEAEGIPAEVQYPS
                     MNRYDLFQPSRSRLPVAVEFAERLDPASMRFPVAEDAGQRRSIYLQENAFRAGREGVE
                     GILEALAKVRKFAGELNRSGTGPR"
     gene            complement(3646..4107)
                     /locus_tag="VE646_06185"
     CDS             complement(3646..4107)
                     /locus_tag="VE646_06185"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HZD79611.1"
                     /translation="MPYEISTKELPPQHVALVRRATSMAVIGKDIHEGFADLMEAMAG
                     AGVSPSGPPFVVYHGLDDQIDRIEICAPVVEPFEDRGEARGMRIPAGLVACTIHRGRF
                     EEIGPAYQALAVWVREHDRQMAGPPREVYLTDLEATPDPASNVTEVQFPID"
     gene            complement(4173..5444)
                     /locus_tag="VE646_06190"
     CDS             complement(4173..5444)
                     /locus_tag="VE646_06190"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HZD79612.1"
                     /translation="MGVKVAVVGAGSTYTPELVEGFARRAGSFPMDELALLDIDPERL
                     DIVGSLAGRMLARAGWSGKLRPTQDRLEAIEGADYVIVQLRVGGQAARYVDETLPLRF
                     GCIGQETTGPGGFAKALRTVPVVLELAELTASRGAPDAWVVDFTNPTGIVTQALLDDG
                     HHAIGLCNVAIGFQRRFAQHFGVEPERVQLDHIGLNHLSWERAVRVDGVDRLPEILEG
                     SAQMVADETDLPVELIRMLGAIPSYYLHYYYLTDEVLEHQRSHRTRAEEVMEIEAGLL
                     EMYRDPDLAEKPKLLEERGGAFYSEAAAQLVASLHAGTGDVQVVDVRNEGAIPGLPDD
                     AVVEVPATIARDGARAAPLAPLAPDMLGLVQHAKAYERLTVSAATSGDRTLALRALMA
                     NPLVRDYRTAEPLLDALLEANRRHLPRFFPD"
     gene            5469..>6241
                     /locus_tag="VE646_06195"
     CDS             5469..>6241
                     /locus_tag="VE646_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HZD79613.1"
                     /translation="MKSLRNFPDPFLGEIAEQPDAMRRAAIALLERRSTLERIREAGR
                     SAHTLIFTGMGSSYDACYPAVNDLAGRGVPALLVDTAELLHFRRPVLDSATLLTIVSQ
                     SGESAEIVKLVSEIAKQRTSPVVLSITNGLANDLAKRATLTLDTAAGRETGPSTMTFA
                     ASMATLSGVARLLAGDSVDTAIDRTRTAAEAAARAVERLVADADAWAQELVDMLGDKE
                     VTVVLGRGPARAAAEMGALMLKECGVMAESLESAAFRHGP"
BASE COUNT          924 a         2305 c         2130 g          882 t
ORIGIN      
        1 gtccatgacc ggcaccaccg tcgcgatggc cagggcgatg taccggatgg ccacctccac
       61 cacggccgcc gcatcctgat cccctctccg cgccgccgag aagacggttc gaagcgacag
      121 cggggaccgc aggcctcggg accgcgcgaa cgtcaggagc cccttggccc caagcacgga
      181 ctcgagcgcc ccatgcctgc ggctggccgg gtggtggggg tcggctacga ggggcaggta
      241 cgcgatctcc cccgcgatcc ccgtcgcccc tcggtacagc tcgccgttca ccacgagccc
      301 gagccccacg ccggtcccca cgtgcaggta cacgaagttg tcgacgccct tgcccaggcc
      361 cttccagtgc tccccgaccg tggccaggtt cacgtcgttc tcgaaggcgg tcttggtccc
      421 cagctcctcc tgcacggctt cgatcagccc gtgccgtccc catcccggca ggttgtgggc
      481 caggaccacc tggtcccgag ccggccgcac gacgcccgga ctgcccacca cggcgaaggt
      541 cacctggcgc cagcgcagcc cagcctcgcc ggcgaggccg tgcgccaccc gcccgatctg
      601 ctcgatcagc gaccgggcgc tgcgggcctt cgcccgctcg tcacgacggg cgacgatgtc
      661 gccggtcagg ttcgcgatgg ccgcccggag ccaggcccgg ccgacgtcga tgcccaccac
      721 ccagcccgcc gtggcatcga gctcgtacag cagggcgctc gggcccttgc caccggtgga
      781 gcggcccgcc tcgcgcacca ggccggtgcg ctccagcgcc gcgagcgcct gggagacggt
      841 gggcttggag agcccactgt cgcgggcgat ctgggctcgg gacacagggc cgcgttgacg
      901 gatgaactcg agcacggtcc gctcgttcat cgccctgagc aagctcggcg tgcccgctgc
      961 gaagatctgc tcgccgcccg tgcctcggcc ccccgccagc gcagtgctaa ggaaactttc
     1021 ctaactattc ggggcgatgc tacgctcccc cggcggggac ggtcaagaga ggcgggcgaa
     1081 ctgcgggagc caggcggcat gggcggtgag gaactcctcg gtgagggcct ccgcggtctc
     1141 cagggagcgg accagcgggt gggccagcag ggcccggacg caggcctccc gctccccatc
     1201 cacggcggcg tccgcggcca gcctcgcgtg ctcgaccacc tgtcggacca gcccggccac
     1261 catcgccggc atcgcaccct gcgcgagggg ttggatggtc cggcccatca cccggcacgg
     1321 gacctccacc accgcgtcgg cagggaggtc ggtgatcgct ccccggttgg gcacgttgag
     1381 gatgaaccgc ccgggccgcc ccgagagcat cgcggccatc acctcgaccg cgaagtcccc
     1441 gtgctcctcg ctggcccgtg ccatgctggg acgcgggtcg gccgcatcgg cctcgcgccg
     1501 gtagctctcc agcacctccg gcagctgagc catgatctcc ccggcccggg tgcgaccctt
     1561 ggagcgttgc tcggccagca cctggtcgtg gaagaagaag tactgcaggt actcgctggg
     1621 gatcagccgg agcacccgga acagccgcac gatcctgcgc tcggcctcgg tggccagcga
     1681 tcccaggtcc agcccatcca gcaggttcca gacccgcccc gtcacgtcca cgccgtccag
     1741 gcgcaccgca cgggtgaagg tcatgtggtt cgtcccggac gtgtcgatct ccagcgcacc
     1801 acggttcacc cccatgagcc tggccaggaa cccgatctcg ccggaggtct ggtcgcacag
     1861 cccgatgaac gggacatccc cgtatcgcac cagggcgtcg gtcaccacct ggaccgggtt
     1921 cgtgtagttc aggatcaccg ctcccggcgc tccgaccttc cggacctcct tcgcgatgtc
     1981 caggagcacg gggatcgagc gcagggccat ggcgaacccg cccgggccgg cggtctcctg
     2041 gccgatgacc ccgtaccggg gcgggatcga ctcgtcgagc tgacgggccg ggaacccgcc
     2101 cggacggaag gcggtgagca ccatgtcggc gccctccaac gctccggccc ggtcgcggga
     2161 cgcgtccacc cgaacccccc ggacaccccg ggaccgcagg atgctgccgc tcagccgtgt
     2221 ctgcaggtcg agcgcgtcgg gatcgatgtc gtgcagcgtc accgtggtgt cggggaagtg
     2281 gtcggcgagc ttcgcgaagg cgtacgcgat gcccggcatg taggccgacc cgccaccgat
     2341 cacgcagatc ttcgtcacgc gccgctccga gcggacctta gcaccggccc ggcggtgagc
     2401 cgggccccag gaaggagctg agcatggccg agcttgcgat caacggcggg tcaccggtcc
     2461 gtccccaggg gttcccggcg tggcccgagt gggacgaacg cgaggtccgg gccgtgaccg
     2521 aggtggtccg cagcggcaac tggggcgggt tccccgagcc cggccccaac gcctcggcgt
     2581 tcgaggacgc gttcgccgcc taccaggggg cggcccacgg gatcctcatg gtgaacggga
     2641 ccatcacgat ggaggtcgcc ctgaaggccc tggacatcgg atggggcgac gaggtcatcg
     2701 tcccggcgct caccttcgtg gccaccgcct acgctccgat ggcggccggc gccctgcccg
     2761 tcatcgtgga cgtcgatccg aggacctgga cgatcgatcc cgaccaggtg gaggcggcca
     2821 tcacgcccag gaccaaggcc atcatgccgg tccacctcgg tcatcagatg gccgacatgg
     2881 accggatcat gaagatcgct cagcagcact cgctggcggt ggtggaggac tgcgcgcatg
     2941 cccacgggca gcgctggcgg gaccggggcg cagggtgcat cggcgacctc gggtccttca
     3001 gccaccagtc ctcgaagatc ctgaccgcgg gcgagggcgg ctcgctgctc acgaacgact
     3061 cggggctggc ctcccgcgcc cattccatca tcgactgcgg ccgccccaag gatcccgaag
     3121 agcgacggta cacgttcggg gcgaactacc ggttgggcga gctgcacgcc gccctgctgg
     3181 tgacccagct ggagcggttc gaggatcagc ggcggcagcg cgtggagggc gccgaggtct
     3241 tcgaggagct cgccccacag gtccccggcg tgagcatcat gccccacgat ccgagggtca
     3301 cccggtggag cttctacaac tacgtgttcg cgatcgagcc ggaggagttc gcgggcgcga
     3361 cgaacgagac ggtcgccgcg gccctcgagg ccgagggcat cccggccgag gtccagtacc
     3421 cctcgatgaa ccgctacgac ctcttccagc cctcgcgctc acgcctaccg gtcgccgtgg
     3481 agttcgctga gcggctcgat cccgcgtcga tgcggttccc ggtggccgag gatgccggcc
     3541 agcgtcgctc gatctacctg caggagaacg ccttccgcgc ggggcgcgaa ggggtggagg
     3601 gcatcctcga ggccctggcg aaggtgcgca agttcgccgg cgagctcaat cgatcgggaa
     3661 ctggacctcg gtgacgttcg acgccgggtc cggcgtcgcc tccagatcgg tcaggtagac
     3721 ctcgcggggc ggccccgcca tctgccggtc gtgctcgcgc acccataccg cgagcgcctg
     3781 gtacgccggc ccgatctcct cgaacctgcc gcggtggatg gtgcacgcca ccagacccgc
     3841 cgggatccgc atcccccggg cttctccccg atcctcgaac ggctccacca cgggagcgca
     3901 gatctcgatc cgatcgatct ggtcgtccag gccgtggtac acgacgaacg gaggtccact
     3961 cgggctgacg cccgcgccgg ccatggcctc catcagatcg gcgaacccct cgtggatgtc
     4021 cttgccgatc accgccatcg acgtcgcccg tcgtaccagg gcgacgtgct gagggggaag
     4081 ctccttggtg ctgatctcgt acggcatgtc gcctccttcc gccgaagagt caagcgccgg
     4141 accgggcgtg ggtctggagt cgttggaccc ggtcagtccg ggaagaagcg cgggaggtgc
     4201 cgccggttcg cctccaagag cgcgtccagc agcggctcgg ccgtccggta gtcgcgcacc
     4261 agcgggttgg ccatcagggc cctcagcgcg agcgtccggt cgccgctggt ggccgcggac
     4321 accgtcagcc gctcgtaggc cttggcgtgc tggaccagac cgagcatgtc gggggcgagc
     4381 ggagccaacg gggccgcacg agccccgtcg cgggcgatcg tggccggaac ctccaccacc
     4441 gcgtcgtcgg gcagaccggg gatcgcgccc tcgttgcgca cgtccacgac ctgcacgtcc
     4501 ccggtgccgg cgtgcagcga ggcgaccaac tgcgccgcgg cctcgctgta gaacgccccc
     4561 cctcgctcct cgagcagctt cggcttctcc gccaggtccg gatcgcggta catctccagc
     4621 agccccgcct cgatctccat gacctcctcg gcccgggtcc gatggctccg ctggtgctcg
     4681 agcacctcat cggtcaggta gtagtagtgc aggtagtagg aggggatggc gccgagcatc
     4741 cggatcagct ccacgggcag gtcggtctcg tccgccacca tctgcgccga gccctcgagg
     4801 atctcgggca ggcggtccac tccatcgaca cggacggcgc gctcccagga caggtggttc
     4861 aggccgatgt ggtccagctg gacccgctcg ggctcgaccc cgaagtgctg cgcgaaccgg
     4921 cgctggaagc cgatcgccac gttgcagagc ccgatcgcat ggtgcccgtc gtccagcagg
     4981 gcctgggtca cgatcccggt cggattcgtg aagtccacga cccacgcgtc cggcgcgccg
     5041 cgcgaggcgg tgagctcggc cagctcgagc accaccggca ccgtgcgcag cgccttggcg
     5101 aacccgcccg ggccggtggt ctcctgcccg atgcagccga accgcagggg cagcgtctcg
     5161 tccacgtacc gtgccgcctg gccgcccacg cgcagctgca cgatgacgta gtcggcgccc
     5221 tcgatggcct cgaggcggtc ctgggtcggc cggagcttcc cgctccagcc ggctcgcgcc
     5281 agcatccgtc cggccagcga gccgacgatg tcgagacgct ccgggtcgat gtccaggagc
     5341 gccagctcgt ccatggggaa cgagccggcc cgccgcgcga agccctcaac gagctccggc
     5401 gtgtaggtgc tcccggcgcc cacgaccgcc accttcacac ccatcgcccc gcatcgtaca
     5461 ctgcccccgt gaaatcgtta agaaactttc ctgatccgtt cctgggggag atcgccgagc
     5521 agccagacgc gatgcggcga gccgccatcg cgctgctgga gcggcggagc accctcgagc
     5581 gcatccgcga ggccggacgg tcggcgcaca cgctcatctt caccgggatg ggcagttcct
     5641 acgatgcctg ctatccggcc gtgaacgacc tcgccggccg aggcgttccc gccctgctgg
     5701 tggacacggc cgagctgctg cacttccggc gccccgtgct ggactcggca acgctcctca
     5761 cgatcgtgag ccagtccggg gagagcgcgg agatcgtcaa gctggtctcg gagatcgcca
     5821 agcagcgcac gagcccggtg gtactgtcga tcacgaacgg tctggccaac gacctggcca
     5881 agcgggcgac cctgaccctg gacaccgctg ccggccggga gaccggcccc tcgaccatga
     5941 ccttcgccgc ctccatggcc accctgtccg gggtggccag gctcctggcg ggcgacagcg
     6001 tcgacaccgc gatcgaccgg accaggacgg cggccgaggc ggccgccagg gcggtcgagc
     6061 gcctggtggc cgatgccgac gcctgggccc aggaactggt cgacatgctg ggcgacaagg
     6121 aggtgaccgt cgtcctgggc cgcggcccgg cccgtgcggc ggccgagatg ggagcgctca
     6181 tgctcaagga gtgcggcgtc atggccgaat ccctggagtc cgccgccttc cgtcacggtc
     6241 c
//
LOCUS       DASQQQ010000151         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_284602, whole genome shotgun sequence.
ACCESSION   DASQQQ010000151 DASQQQ010000000
VERSION     DASQQQ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284602"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..606)
                     /locus_tag="VE646_06200"
     CDS             complement(292..606)
                     /locus_tag="VE646_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004955155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79614.1"
                     /translation="MSGEFLSAAGDIQPIIGGLAPNWGPFGKLGAEARVMIEVIMAAA
                     VLVCLGIAAWGAAKQRIGSTALRDTFSAEQGKGLIIAGLTGVFIIGSLGTIFTIVYGM
                     AI"
     gene            958..1917
                     /locus_tag="VE646_06205"
     CDS             958..1917
                     /locus_tag="VE646_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018840932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HZD79615.1"
                     /translation="MPAKSLVAAAVVTPVLFMGGLGAATIASAAGSAGTTLVQGTVPE
                     GLAPYLERYGHTCPELTPGRLAAQLYQESGFRTDVVSSANAQGLAQFLPGTWAAHGRD
                     GDGDGRAVIWNPADAVRSAAEYDCSLAGWGRGLPGDHVNNMLAAYNAGYGRMKLLYQG
                     GTPWPAETQNYVRRIRALERAFTAVRGPVPPSRAAAIAIQYARSKLGTPYLWGGTGTV
                     EQGGRFDCSGLMQAAYRAAGLNLSRTSRTQWYDGPHVPREQLQPGDLVFFAWDTTSPA
                     SIHHVGMYVGGGYMINAPYTGAVIRFDKIDTPGYIGAVRPTAA"
BASE COUNT          318 a          753 c          727 g          329 t
ORIGIN      
        1 gggtgcgggg ctgttcgtcg gtgacgggcc cgtagtcagg cggcatggcc agatcccttc
       61 aggctgatgc cgggggagcg agcccgctgt ggacggcgcg gcgtaccggc ggccggggca
      121 gcctcacccc gcccgtccga acggcaccga tccctggcgg aggctgggca gcggccgtct
      181 ccggacagga tgacgccggt ggaccgctgg cgccccccgc ctccccttcc ggcccggtca
      241 tcctgtctcg ccgacgtgcc cgcaggctcc ccacagcagc cttttccgtg cctagatggc
      301 catcccgtag acgatcgtga agatcgtccc gagcgagccg atgatgaaca ctccggtcag
      361 gccggcgatg atcaggccct tgccctgttc tgcgctgaag gtgtcacgga gcgcggtcga
      421 cccgatccgc tgcttggcgg cgccccaggc ggcgataccc aggcatacca ggacggccgc
      481 tgccatgatg acctcgatca tcacccgggc ctcggcgccg agcttgccga acggtcccca
      541 gttgggtgca aggccaccga tgatgggctg gatgtcgcca gcggcagaca gaaattcacc
      601 actcatcaga cgcccccgat cctgcgtctc gtgtccgtcc gagcggacgg atctttacgt
      661 tcatctgcct cgccgtcatg cgctttccgg cgaggccgtg gacagacgtc tcctgtgcgg
      721 ctatccacgt tcactctgtg tatcacgggc aattgcgtac agcaacaagg tgtcttacaa
      781 aggataagcg atgcggtggc ggtcctcgtc ctgcgttacc tcccgctgag gcggtagcct
      841 ggtccgtcca gcgcgcgacg ccgacgtatc caccaggccg ggggtgccgc acatgcagga
      901 cacgggaggg cccacacgac gctccagcga tggtatacag cgcggcgggg cttgaccatg
      961 ccggcgaaat cgctggtcgc cgccgctgtg gtcacgccgg tcctgttcat ggggggtctg
     1021 ggggcggcga ccatcgcgtc cgcggccggg tccgccggga ccacgctggt gcagggcacc
     1081 gtgccggagg gcctggcgcc gtacctcgag cggtacggcc acacctgccc ggagctgacc
     1141 cccggccggc tggccgccca gctctaccag gagtccggct tccgcaccga cgtggtgagc
     1201 agcgccaacg cccagggcct ggcccagttc ctccccggca cctgggccgc ccacggccgg
     1261 gacggtgacg gggacggccg ggccgtcatc tggaacccgg ccgacgcggt ccgctcggcc
     1321 gccgagtacg actgctcgct ggccggctgg gggcgcggcc tccccggcga ccacgtgaac
     1381 aacatgctgg ccgcctacaa cgccggctac gggcggatga agctgctgta ccaggggggc
     1441 accccgtggc cggccgagac gcagaactac gtgcgccgga tccgcgccct ggagcgggcg
     1501 ttcaccgcgg tgcgcgggcc ggtcccgccg tcccgggcgg ccgcgatcgc gatccagtac
     1561 gcccggtcca agctcggcac gccgtacctg tggggtggca ccggcaccgt ggagcagggg
     1621 ggccggttcg actgctcggg tctgatgcag gccgcctacc gcgcggccgg tctcaacctc
     1681 agccggacct cgcggaccca gtggtacgac gggccgcacg tcccgcggga gcagcttcag
     1741 cccggcgatc tggtgttctt cgcctgggac accacctcgc ccgcctccat ccaccatgtc
     1801 gggatgtacg tcggcggcgg gtacatgatc aatgcgccct acaccggggc ggtgatccgt
     1861 ttcgacaaga tcgacacccc gggttacatc ggggcggtac ggcctaccgc agcgtgaaac
     1921 ggcgccattc cggtgatcac gacgtcgtga tcaggtagtc acatttcgcc acgctgcgta
     1981 ctgacttgcc atcatcgagt cactcactcg actgtgtgaa tgacgaaggc ttcccccggc
     2041 tcggaccaag ctgacatatt cgaccgaaga ggagtccggc ggtgacatgc gcgggacggg
     2101 gagatcagcg cccgggcggt cccggcc
//
LOCUS       DASQQQ010000152         4959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_68314, whole genome shotgun sequence.
ACCESSION   DASQQQ010000152 DASQQQ010000000
VERSION     DASQQQ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68314"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1603
                     /locus_tag="VE646_06210"
     CDS             <1..1603
                     /locus_tag="VE646_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HZD79616.1"
                     /translation="WEDAAPTDRYEMRSICLALIDSDILDDERLAIDVRGKRLEAVVV
                     PFHLRSEAPPYARPILASHEEPPQSVATNPVAARVRRLLERAAENTEWRQEACINLIP
                     SEMTASMLVRLLSIMDPAFRYAEHRPLEAFYEEEVFYYQGTGFIGQVEQQVERELATF
                     LGCREVEARVISGQMANGAVFSGLVDYRNRTDRRREPGRIARVMNHHIGKGGHLSAQP
                     MGALRDFVARDPSTERPAVLNFPVLPENPYRVDVPATLELIDTYRPELIVLGKSVIIH
                     REPVEPIRRFLDEQAIPAVLMYDMAHVLGLVGPHFQRPFDEGADLVTGSTHKTFFGTQ
                     RGVIGTRFEEDEERYELWEAIRSRTFPGSVSNHHLGTLVGLLMATYEMQRFKDEYQPA
                     VIANAKAFARALADCGLSVAGDPSIDFTETHQVVVDVGYGRGPSIAARLEANNIICNY
                     QARPQDEGFTAAGALRLGVAEMTRFGMEPDDFRELASLMRDVIVADADVAERVRELRK
                     RFREMRYCFGTEDFPDLTRRLSGLV"
     gene            complement(1644..2480)
                     /locus_tag="VE646_06215"
     CDS             complement(1644..2480)
                     /locus_tag="VE646_06215"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HZD79617.1"
                     /translation="MTQLTWAVARAGGLVSWALAAASVLWGLALSTRLLGRRPRPPWL
                     YDLHRFLGGLTVVFIGVHVGAILLDRFVHFGLIQVLVPFTGTWHPTAVAWGIVAMDLA
                     VAVEVTSLLRSRISTRVWRRVHLGSFAVFMLGTVHGLSAGTDALSGPTRWLMVGTSVM
                     VVAMTAVRIASPRRTAPTLRRARVPARPDRSAIRAAAFPRDGRGKNQAMNQAEREDYE
                     FSCGDCGSSWTETYDVTLRTDGTVVTRSFWKEGLPVTSPLFGKACRLCGGHVVNRRRL
                     AA"
     gene            complement(2477..3400)
                     /locus_tag="VE646_06220"
     CDS             complement(2477..3400)
                     /locus_tag="VE646_06220"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZD79618.1"
                     /translation="MAAETRFRAMGSDVHVIVVRGSRALLWAAADRLEDLEARWSRFR
                     PTSEVSLMNAMAGRPVRVSGPTLELVLRSLEGARRTGGRFDPSVLGDVIRAGYDRSFE
                     TLPDDPPPGRSTLRSGYPGIVVDVASRTVRLPEGVGFDPGGIGKGLAADLLAAELSAA
                     GAVGVCANVGGDLRVEGPAPGGGSWPVAIEHPWGGPAITVSLRAGAVATSTRARRVLG
                     SGHHLIDPATGRSAQTGIASATAVAAEAWQAEVLAKAAFLGGDPAWGGAEWLVVEDSG
                     RIRTSPGFSRFVVPMPHGKPGSEPPDGRLAG"
     gene            3502..3738
                     /locus_tag="VE646_06225"
     CDS             3502..3738
                     /locus_tag="VE646_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79619.1"
                     /translation="MPDAWVLDVPVLPAAIPETMPRNAITLIAPAITRERAAAWRRRG
                     RGPDGPFWGSTYVLLPGISTPSEPAEAEDRVGPP"
     gene            complement(3761..3991)
                     /locus_tag="VE646_06230"
     CDS             complement(3761..3991)
                     /locus_tag="VE646_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79620.1"
                     /translation="MIHRCPYCGALAHDDRTEVLDSTDGPILMTRWRCAEDDTHWWTA
                     STDSPVVRRTEPAATGLLGAILRDLEEAGRAA"
     gene            complement(3988..>4959)
                     /locus_tag="VE646_06235"
     CDS             complement(3988..>4959)
                     /locus_tag="VE646_06235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZD79621.1"
                     /translation="AEVAACLRMLGVRSVRLITNNPAKAEGLTRSGLVVEERVPLPTS
                     AHARSMGYLRTKERRLAHLAPAGDPLPDGIQPVTDVTPLLGEVRTPFDRPYVVLKYAQ
                     TLDGRIATRTGDARWISGEPERRMSHALRAACDAVLVGIGTVLTDDPLLTVRMVPGVS
                     PTRVVLDRRLRIPPEAKVLGEGASTVVVTSTGADAARVQDLRRSGVGVIAVGPTEGGL
                     DLEQTLRSLRARGVRTLLVEGGARVITSFLRSGSVDRLIVSLAPTIIGAGTAAVGDLG
                     VTRMMQAVRLPDASVHRVGPDLVVAGSLHRGTSSAETERAPDVSGVA"
BASE COUNT          748 a         1832 c         1719 g          660 t
ORIGIN      
        1 ttgggaggac gcggcgccca ccgaccggta cgagatgcgc tcgatctgcc tggccctgat
       61 cgatagcgac atcctggacg acgaacggct cgcgatcgac gtccggggca agcggctgga
      121 ggccgtggtg gtgccgttcc acctacgctc ggaggcgccc ccctacgccc gccccatcct
      181 cgcctcgcac gaggagccac cccagtcggt cgcgaccaat ccggtcgccg cgagggtgcg
      241 ccgcctcctg gaacgcgccg ccgagaacac cgagtggcgg caggaagcgt gcatcaacct
      301 gatcccctcg gagatgacgg cctccatgct ggtccgcctg ctctcgatca tggacccggc
      361 gttccggtac gcggagcatc ggccgctcga ggccttctac gaggaggagg tcttctacta
      421 ccagggcacc gggttcatcg ggcaggtcga gcagcaggtc gagcgcgagc tcgcgacctt
      481 cctgggctgc cgggaggtgg aggcccgggt gatcagcggc cagatggcga acggggcggt
      541 gttcagcggc ctggtcgact accgcaaccg gaccgatcgg cgccgcgagc cgggccggat
      601 cgcccgggtg atgaaccacc acatcggcaa gggaggacat ctgagtgccc agcccatggg
      661 cgcgctgcgg gacttcgtgg cccgcgatcc ctcgaccgaa cggccggcgg tgctgaactt
      721 ccccgtcctg cccgagaacc cctaccgcgt ggacgtgccg gccacgctgg agctgatcga
      781 tacgtaccgg cccgagctga tcgtgctcgg caagagcgtg atcatccacc gggagcccgt
      841 ggagccgatc cgtcggttcc tggacgaaca ggcgatcccg gcggtgctga tgtacgacat
      901 ggcgcacgtg ctcgggctgg tgggccccca cttccagcgg ccgttcgacg agggggccga
      961 cttggtgacc ggctccaccc acaagacgtt cttcgggacc cagcgcggcg tgatcggcac
     1021 ccgcttcgag gaggacgagg agcgatacga gctgtgggag gcgatccgct cgcggacctt
     1081 ccccggttcc gtgtccaacc accacctcgg caccctggtg ggcctgctga tggcaacgta
     1141 cgagatgcag cgcttcaagg acgagtacca gcccgccgtg atcgcgaacg ccaaagcctt
     1201 cgcccgcgcc ctcgccgatt gcgggctctc cgtggccggc gatccgtcca tcgacttcac
     1261 cgagacccac caggtggtgg tggacgtcgg gtacgggcgc gggccctcga tcgccgctcg
     1321 gctggaggcg aacaacatca tctgcaacta ccaggcccga ccgcaggacg aggggttcac
     1381 cgccgcgggc gccctgcgcc tgggcgtggc cgagatgacc cggttcggta tggagcccga
     1441 cgacttccgc gagctcgcct cgctcatgcg ggacgtgatc gtggccgacg cggatgtggc
     1501 agagcgggta cgcgagcttc gcaagcgctt ccgtgagatg cgctattgct tcggcaccga
     1561 ggacttcccc gatctcaccc gacggttgtc ggggctcgtc tagctacgct ttgtcctcgg
     1621 cgatccgccg ctcgctcatc ccttcaggcg gccagtcgcc gacgattgac cacgtggcca
     1681 ccacacagcc ggcaggcctt gccgaacagg ggggaggtga cgggcagacc ctccttccag
     1741 aaggatcgcg tgaccaccgt tccgtcggtc ctgagcgtga cgtcgtaggt ctcggtccag
     1801 ctcgatccgc agtctccaca ggagaactcg tagtcttctc gttccgcttg gttcatggct
     1861 tggttctttc cacgcccgtc ccgcggaaac gccgcggccc ggatggcgga ccgatcggga
     1921 cgggccggca cccgggcgcg ccggagggtc ggggcggtgc gcctggggct cgcgatccga
     1981 acggccgtca ttgccaccac catcacgctc gttccgacca tgagccaccg ggtcggaccc
     2041 gacagcgcgt cggtacctgc gctcaggccg tgcacggtcc ccagcatgaa caccgcgaac
     2101 gagcccaggt gcacccgccg ccacacccgg gtggagatcc gggaacggag cagcgaggtg
     2161 acctccacgg cgacggccag gtccatggcg acgatgcccc aggccaccgc cgtgggatgc
     2221 caggtgccgg tgaacgggac caggacctgg atcaggccga agtggacgaa gcggtcgagc
     2281 aggatggccc ccacgtggac cccgatgaag accacggtca gcccgcccag gaaacggtgc
     2341 aggtcgtaca gccacggcgg acggggacgt cgaccgagca gccgggtcga cagggccaga
     2401 ccccacagca ccgaggcagc cgccagggcc cagctcacca ggccgccggc ccgagccacc
     2461 gcccaggtca gctgggtcat cccgccagcc tcccgtccgg cggttcggaa cccggcttgc
     2521 cgtggggcat cggcaccacg aaccgggaga agcccgggct agtgcggatc cggcccgagt
     2581 cctccaccac cagccactcg gccccgcccc acgccgggtc accacccagg aacgcggcct
     2641 tggccaggac ctcggcctgc cacgcctcgg ccgcgacggc cgtggccgag gcgatccccg
     2701 tctgcgccga acggccggtc gccgggtcga tcaggtggtg ccccgacccc agcactcggc
     2761 gggcccgcgt gctcgtggcc acggccccgg cccgcaggga caccgtgatc gcggggccgc
     2821 cccacgggtg ctcgatcgca accggccatg agccaccccc cggtgcgggc ccctccaccc
     2881 ggaggtcccc gccgacgttg gcgcagaccc ccacggcacc cgccgccgac agctccgccg
     2941 cgagcaggtc ggcggccaag cccttgccga tgcccccggg gtcgaacccg acaccctccg
     3001 ggaggcggac cgtgcgcgat gccacatcga cgacgatgcc cgggtagccc gatcggagcg
     3061 tcgaccgccc gggtggaggg tcgtcgggca gggtctcgaa gctccggtcg tagccggctc
     3121 ggatcacgtc gcccaggacc gaggggtcga agcgaccgcc ggtccgtcgc gccccctcca
     3181 gggagcgcag caccagctca agcgtcggtc ccgagacccg gaccggccgg cccgccatcg
     3241 cgttcatcag gctcacctcg ctggtgggcc ggaaccggct ccaccgggcc tcgaggtcct
     3301 ccagccggtc cgccgccgcc cacagcaacg ccctcgagcc ccgcaccacg atcacgtgca
     3361 cgtccgagcc catggcgcgg aaccgggtct cagctgccat ggctcgaggt caccggcatc
     3421 tggcccacgg agttgctgcc gggaagcgcc gacccgaacg agctcgaagc cttcgaactc
     3481 ctggaggtgg ggtccgcggt ggtgcctgat gcctgggtgc tcgacgtacc ggtcctgccg
     3541 gccgcgatcc ccgagacgat gccgaggaac gcgatcacgc tcatcgcgcc cgcgatcacg
     3601 cgggagcgag ccgcggcgtg gcggcggcgc ggacgggggc cggacgggcc cttctggggt
     3661 tccacgtacg tgctccttcc gggcatcagc actccaagtg aacccgcgga ggctgaagac
     3721 cgggtgggac ccccctgagg atcgcctgag agctggtgcc tcaggcggcc cggccggcct
     3781 cttccaggtc cctcaggatc gcgcccagca ggcccgtggc ggccggctcg gttcggcgga
     3841 ccaccggcga atcggtggac gcggtccacc agtgggtgtc gtcctccgcg caccgccagc
     3901 gggtcatcag gatgggcccg tcggtggagt ccagcacctc ggtgcgatcg tcgtgcgcga
     3961 gggccccgca gtaggggcat cgatggatca agccaccccg ctcacgtccg gggcgcgctc
     4021 ggtctcggcg gaggacgtcc ctcgatgcag gctccccgcc accaccaggt ccggtcccac
     4081 ccgatgcacg gaggcatcgg gcaaccgcac ggcttgcatc atccgcgtca cccccaggtc
     4141 gcccacggcg gcggttccgg ccccgatgat cgtgggggcc agcgagacga tcagccgatc
     4201 caccgatccg cttcggagga acgacgtgat caccctggct cccccctcca ccagcagggt
     4261 ccggacccca cgggcccgga ggctccggag ggtctgctcc aggtccaggc cgccttccgt
     4321 ggggccgacc gcgatcacac caaccccgga tcgacgcagg tcctggacgc gagcggcgtc
     4381 cgcgccggtc gaggtcacca ccacggtcga agcgccctcg cccaggacct tggcctccgg
     4441 ggggatccgc agccggcggt ccaggaccac ccgggtgggg gacaccccgg ggaccatccg
     4501 cacggtgagc agcgggtcgt cggtcagcac ggtcccgatc cccaccagca cggcgtcgca
     4561 cgccgcgcgg agggcatggg acatgcgccg ctcgggctcc ccggagatcc accgggcgtc
     4621 cccggtccgc gtggcgatcc gcccgtcgag cgtctgggcg tacttcagga ccacgtaagg
     4681 gcggtcgaag ggcgtgcgga cctctcccag gagcggggtg acgtcggtca cgggctggat
     4741 gccgtcgggc agcgggtccc cggcgggggc caggtgcgcg agccggcgct ccttggtccg
     4801 caggtagccc atgctgcgtg cgtgggccga ggtgggcagg ggcacccgct cctccacgac
     4861 caggccggac cgggtcaggc cctcggcctt ggccgggttg ttcgtgatga gccgcaccga
     4921 ccgtacgccg agcatccgca ggcacgccgc gacctccgc
//
LOCUS       DASQQQ010000153         8391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_261017, whole genome shotgun sequence.
ACCESSION   DASQQQ010000153 DASQQQ010000000
VERSION     DASQQQ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8391
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261017"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..125
                     /locus_tag="VE646_06240"
     CDS             <1..125
                     /locus_tag="VE646_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HZD79622.1"
                     /translation="IRTWITDWNENPRPFVWHKTADQILESLAAYCERISDSGH"
     gene            complement(196..546)
                     /locus_tag="VE646_06245"
     CDS             complement(196..546)
                     /locus_tag="VE646_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HZD79623.1"
                     /translation="MPLAAALALGLVACGSSRGGSGQIHEGPGGPDAVKVVMEDSRFE
                     PDPLRLPAGTKVTVGLTNEGDENHNFTIDALHLPTGTMASGDGVSATFTVPTGSTEFH
                     CTFHPGMDGTITGT"
     gene            complement(599..851)
                     /locus_tag="VE646_06250"
                     /pseudo
     CDS             complement(599..851)
                     /locus_tag="VE646_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020518759.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
     gene            complement(885..1175)
                     /locus_tag="VE646_06255"
     CDS             complement(885..1175)
                     /locus_tag="VE646_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HZD79624.1"
                     /translation="MKAVHAVVLVQCDVDTIPEAAQAIADLEGVSEVYSVAGEFDLVA
                     VVRVSDHDRLASVIPGGIAKVHGVVRTETLIAFQVYSRHDLERLFSVGFKEG"
     gene            complement(1172..2350)
                     /locus_tag="VE646_06260"
     CDS             complement(1172..2350)
                     /locus_tag="VE646_06260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZD79625.1"
                     /translation="METAVILEGARTPIGRFLGSLSEVPTVDLGVLAVREALRRADVE
                     PEQVELTIMGHGRQAGNGPNTGRQVSIKSGIPQEVPGYNVNMACASGMKAVQIAAQQI
                     MLGEAEIVVAGGMENMTRVPFLLDRMRFGYRLGDATVYDAMYRDGLLDPLCGLVMGET
                     AENLVDRYDISRAEQDAFALESQQKAEGGKARRAAEMFPVEAPNPRGKGTITLAQDEH
                     PRPETTLEKLAALPPVFRKDGSVTAGNSSGLTDGAGAVVLMSESRAKAEGRTILARIL
                     GGSSAGVDPSIMGVGPVPATRKVLEKTGLSLSDMECIELNEAFAAQVIACERELKFDR
                     DRLNPKGGGISVGHPIGLSGTRIILTLAYEMRELGYALGLATLCVSGGQGAAVVLERA
                     "
     gene            complement(2360..4045)
                     /locus_tag="VE646_06265"
     CDS             complement(2360..4045)
                     /locus_tag="VE646_06265"
                     /inference="COORDINATES: protein motif:HMM:NF005907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HZD79626.1"
                     /translation="MFDILGAPRGPAGGAVLEPVQTTREEQIPLFAVTFCVVDLETTG
                     GSPTDARITEIGAVKYRGGERIGVLETLVDPGIPIPPFITHLTGIRDQDVRGAPGIGA
                     VLSSFEEFVAGCVFVAHNARFDFSFLNTALARLDRDPLPPPPVCTARLARRVVWPDVP
                     NVRLQTLAGYFRTSVRPTHRALADAQACAEVLHGLLELGGRLGIASLGDLHQAVRARG
                     RPNFGKIRLADGLPRRPGVYLFRGRDGRVLYVGKSKDLRSRVRSYFYGDPRKKVEDLL
                     TQTSSVEGLPCDSELEALVLEARLIRLHEPAYNRRGRTWRRYAYLRIDTSDAFPRIKI
                     VREATTGGTFLGPFPSSQLARMAKEALEEAFPIRRCTRPMRASTRSAPCALSDMGRCL
                     APCDGRVGPERYGELVRSLISSLTHPGGLLGALERQLRRLADQERFEEAALARDRLRA
                     LAEALSRRRQDAWLLGPGRLRLSDRAGAELSFRDGALEVGTPADPISYPYPRAQADEV
                     AAVRNWLQRNEVRLERADVPLSEPLEGRPLRDVLVLLRSSGSDRGSGPRAADR"
     gene            complement(4079..4663)
                     /locus_tag="VE646_06270"
     CDS             complement(4079..4663)
                     /locus_tag="VE646_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004595167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HZD79627.1"
                     /translation="MNASWVRGYLEGTARTARHAAEGCAEDVAAAAELLVNAVLDGGK
                     ILICGNGGSAADSQGLATEFVSTLTLDNRRPAIPALALTTDTSVLTAVANDFGFEGVF
                     ARQVEALGHPGDVLVGISTSGNSEDVVRAFEQAAAQSIHRVLLSGATGGRLASLADVA
                     ILIPSGETSHIQEGHLAVEHALAFLVERTLYPRG"
     gene            complement(4660..5235)
                     /locus_tag="VE646_06275"
     CDS             complement(4660..5235)
                     /locus_tag="VE646_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZD79628.1"
                     /translation="MDAAAMSARFVLLDRDGTINEDVDHLLDPAQVRLIPGAAEAIRM
                     LRDLGLGIVVVTNQSPVGRGWISMTELDAIHARLVDQLMRRGATIDGIYACPHGPEAG
                     CACRKPATGLALRAAAEHGFDPSQSFVVGDHRGDMELGRALGATSILLLTGHGRQELE
                     HGAGEVADAVVADLPAAAEVIRSMVANGAAP"
     gene            complement(5217..6257)
                     /locus_tag="VE646_06280"
     CDS             complement(5217..6257)
                     /locus_tag="VE646_06280"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HZD79629.1"
                     /translation="MSDREIRAAAPLRISFVGGGTDFPHWYERHGGAVLSATIDRFAR
                     VSLSPRSDARVRLRDLRLDQHAQYHLREGPTFDGVLDLPKAAVRRLGLDRGIDLELRS
                     DAPPGSGLGGSSAMVTAVVAALAAFGDIEISAHELAHLSYTIERNDLGIAGGWQDQYA
                     AAFGGFNLIEFAASGVAVTSVRLSGGTIDALRRHLLLCYTGGVRTNLGLIDTQIRLYR
                     EGREETIQGMKQLQAMAYEMRDVLEAGDVGSLGPLLQEAYESKRRMNPHIADGTAIDE
                     LLARARAAGATGGKICGAGGGGYLLLSCPPEVRSRVRRQLEALGARFSPFDFSEGGVE
                     ARTGSDRWTPRR"
     gene            complement(6305..6583)
                     /locus_tag="VE646_06285"
     CDS             complement(6305..6583)
                     /locus_tag="VE646_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HZD79630.1"
                     /translation="MSRESNGGIDLKTLPRDPSPPDMWQEDAACYGIEPDVFFPTSEE
                     EAGPALAYCSGCPIRETCLAWALKNGERYGVWGGLTEQQRRRLVRFVA"
     gene            complement(6718..7581)
                     /locus_tag="VE646_06290"
     CDS             complement(6718..7581)
                     /locus_tag="VE646_06290"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79631.1"
                     /translation="MDERTLEEQISALDDARALVAWSDLRLTSVVGTDARRWLHDLVT
                     ADIAGLEAFSVRRCLLLTPSGHIRADVHVYGMEDGTFVLAQSADQPAPVADLLEPYVL
                     SSEVGLEPCPLGVLSVPGPWAVPAELPAIWSPSVLGDGFDVLGPGEELAAARRLLSED
                     RVEVSARAVEAWRILRGLPRFPVDVDQTSVPAEAGLDETVDTTKGCFLGQESVAKIRN
                     LGHPPRVVLALHADGPVAPGATVDAAGERAGLVTSATPDGRGGTALLGRVRWERRQGA
                     LTASGLRLERV"
     gene            7606..>8391
                     /locus_tag="VE646_06295"
     CDS             7606..>8391
                     /locus_tag="VE646_06295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZD79632.1"
                     /translation="MGLPRTLLVTNDFPPRVGGIQRTLHALWRELPADRVSVFAPASE
                     GSPRFDGGEPYVIRRGDRGFLWPTTRTRDAILAAAGEVEAEVVLFGAAYPLALLGPAL
                     SRAGLRYVTAAHGFEYWLSLAPGTHALMRRATSRACHVAVMCSAFIARVVRTAVPERV
                     PVSVMYPGADVEAFRPDLPTEDLRRVHGLGARPVVVCVSRLVPRKGQDVLIRAMPQIR
                     QRVRDAALLVVGDGPHRARLERLAEEHAPRSVVFAGQVSEADLP"
BASE COUNT         1211 a         3026 c         2973 g         1181 t
ORIGIN      
        1 cgatccggac ctggatcacc gactggaacg agaacccgcg gccgttcgtg tggcacaaga
       61 ccgcggacca gatcctcgag agcctcgccg catattgcga gcggatctcc gactcaggac
      121 actaggggag gacgtcatcg ggaaggtatg cgcgccttcc aggatggatg cgccacgtct
      181 gggagacggg acggcctagg tccccgtgat cgtgccgtcc atgccgggat ggaacgtgca
      241 gtggaactcc gtggagccgg tcgggacggt gaacgtcgcg ctcaccccgt ctccagacgc
      301 catcgtgccg gtgggcagat ggagcgcgtc gatggtgaag ttgtggttct catccccctc
      361 gttcgtgagc ccgacggtga ccttcgtccc ggcgggcagg cggagcggat cgggctcgaa
      421 ccgcgagtcc tccatcacga ccttgacggc gtcggggccg ccgggtccct cgtggatctg
      481 tcccgacccc ccgcggctcg atccgcaggc gaccaggccc agggccaggg ccgcggcgag
      541 cggcaaccac cagcgctaca tccgtgcctc cttccggctc tcgttcctcg gtgcgggcct
      601 agcgatcctc gaggtggcga cggacggcca ggactcgtcg gtgctcggtg atcaccagcc
      661 ggccggcgat gcccgcgttg agaggccctc cgccatcagc gccaagacct cctcctcgcg
      721 cggcgtcagc cgatggtgcg ggtgccggcg agcccgagcc aggagcgccg tgcaaccgtc
      781 ggccacccgg atcagggcgt ccaggaagtc gcggacgtcg gccacgcctg cttgagtagg
      841 tagccgaccc cgtggtgtcc ccggacagtg gatcgctcgg gggactagcc ctccttgaag
      901 ccgaccgaga acaggcgctc caggtcgtgc cgggagtaga cctggaacgc gatgagcgtc
      961 tcggtccgga ccacgccgtg aaccttggcg atccctccgg ggatcacgct ggcgagccgg
     1021 tcgtgatcgg acacccggac cacggccacc aggtcgaact ccccggccac cgagtagacc
     1081 tcggagaccc cttccaggtc ggcgatggcc tgagcggcct cggggatcgt gtccacgtcg
     1141 cactggacga ggaccacggc gtggacggcc ttcacgcgcg ctccaacacc acggccgccc
     1201 cctggccgcc cgacacgcag agcgtggcaa gcccaagcgc gtagccgagc tcgcgcatct
     1261 cgtacgccag ggtcaggatg atccgcgttc ccgagagccc gatggggtgc cccaccgaga
     1321 tgccgccgcc cttggggttc aggcggtcgc ggtcgaactt cagctcgcgc tcgcaggcga
     1381 tcacttgggc cgcgaacgcc tcgttcagtt cgatgcactc catgtccgac agcgacaagc
     1441 ccgtcttctc gagcaccttg cgggtggccg ggaccgggcc gacgcccatg atcgacggat
     1501 cgaccccggc cgagctcccg cccaggatcc gggccaggat ggtgcggccc tccgccttcg
     1561 cccgggactc ggacatcagg accacggcgc cggcaccatc ggtgaggccc gaggagttcc
     1621 ccgccgtgac cgagccgtcc ttgcggaaca cggggggaag ggccgcgagc ttctccaggg
     1681 tggtctcggg acggggatgc tcgtcctggg ccagggtgat ggtgcctttg cccctggggt
     1741 tgggggcttc caccgggaac atctccgccg cccgtcgcgc cttcccgccc tcggccttct
     1801 gctgggactc cagcgcgaac gcgtcctgtt cggcacgcga gatgtcgtag cggtcgacca
     1861 ggttctccgc ggtctcgccc atcaccagcc cgcagagcgg gtccagcagc ccgtcccggt
     1921 acatcgcgtc gtacacggtg gcatcgccca ggcggtagcc gaagcgcatc cggtccaaca
     1981 ggaacgggac ccgggtcatg ttctccatgc cgccggccac gacgatctcg gcctcgccta
     2041 gcatgatctg ttgggcggcg atctggacgg ccttcatccc cgatgcgcac gccatgttga
     2101 cgttgtagcc gggcacctct tgggggatcc cgctcttgat cgagacctgg cgtccggtgt
     2161 tcggtccatt gccggcctga cggccgtgtc ccatgatcgt gagctcgacc tgctcgggtt
     2221 ccacatcggc gcgccggagc gcctcgcgca cggcgagcac ccccaggtcc accgtgggga
     2281 cctcggagag ggatcccagg aacctgccga tcggtgtccg ggccccctcg aggatgacgg
     2341 ccgtctccac ggcaccagtc tatcggtccg cggcgcgggg ccctgacccg cggtccgagc
     2401 cggacgagcg caggaggacc agcacgtctc gcaacggccg gccctcgagc ggctccgaga
     2461 gtggcacatc ggcccgctcc agtcggacct cgttgcgctg caaccagttc cggacggctg
     2521 cgacctcgtc ggcctgggcc ctggggtagg ggtaggagat gggatcggcg ggggtcccca
     2581 cctccagcgc tccgtcccgg aagctgagct cggcgccggc gcggtccgac aggcgcagcc
     2641 gccccggccc gagcagccag gcgtcctggc gacggcggga taacgcctca gccagtgcgc
     2701 gcagccggtc ccgagccagg gcggcttcct cgaaccgctc ctggtccgcg agccggcgca
     2761 gctgccgctc gagcgctccg aggagcccgc ccgggtgtgt gagggaggag atgagggacc
     2821 ggacgagctc cccgtaacgc tcgggcccga cgcgaccgtc gcacggggcc aggcaccggc
     2881 ccatgtcgga cagggcgcac ggggcggaac gggtgctggc ccgcatcgga cgggtgcacc
     2941 ggcggatcgg gaaggcctcc tccagggcct ccttggccat cctggccagc tgtgaggagg
     3001 ggaacggccc caagaaggtc ccaccggtgg tggcctcccg gacgatcttg atgcgcggga
     3061 aggcgtcgga ggtgtcgatg cgcaggtacg cgtaccggcg ccaggtcctg ccccgacggt
     3121 tgtacgccgg ctcgtggaga cggatcaggc gagcctccag caccagggcc tcgagctccg
     3181 agtcgcaagg cagcccctcc acggaggatg tctgcgtcag caggtcctcc accttcttcc
     3241 ggggatcgcc gtagaagtag ctccggaccc gcgagcgcag gtccttggac ttgcccacgt
     3301 agagcacgcg tccgtcccgg ccccggaaca ggtagacgcc cgggcgccgg ggcaggccgt
     3361 ccgccagtcg gatcttcccg aagttcggcc ggccgcgagc ccgcaccgcc tgatgcaggt
     3421 cgccgaggct cgcgatcccc agtcgacccc ccagctccaa gagcccgtgg agcacctcgg
     3481 cgcaggcctg tgcgtcggcc agggcgcgat gcgtgggccg gaccgaggtg cggaagtagc
     3541 ccgccagggt ctggagtcgg acgttcggga cgtctggcca caccacgcga cgggccagcc
     3601 gagcggtgca gacgggcggc gggggcagcg ggtcgcgatc cagccgcgct agggcggtgt
     3661 tcaagaacga gaagtcgaag cgcgcgttgt gggccacgaa cacgcagccg gccacgaact
     3721 cctcgaagga ggacaggacc gcaccgatgc cgggcgcgcc gcggacgtcc tggtctcgga
     3781 tcccggtcag gtgggtgatg aacggcggga tcgggatgcc gggatcgacc agcgtctcca
     3841 gcacgccgat ccgctcccca ccccggtact tgacggcccc gatctcggtg atgcgcgcgt
     3901 cggtgggcga tcccccggtg gtctccaggt ccacgacgca gaacgtcacg gcgaagagcg
     3961 ggatctgctc ctcccgcgtg gtctggaccg gctcgagcac cgcacctccc gctgggccgc
     4021 gcggggcgcc caggatatcg aacacctgtt cgatggtcaa gcccgggggc ccggtggctc
     4081 agccgcgcgg atacagggtg cgctccacca ggaaggcgag ggcgtgctcg accgccagat
     4141 gcccctcctg gatgtgggag gtctcgccgc tcgggatcaa gatcgcgacg tccgccagcg
     4201 aggccagccg gcctcccgtc gcgccgctca gcaggacccg gtggatgctc tgggccgccg
     4261 cctgctcgaa ggcccgcacc acgtcctcgg agttcccgct ggtcgagatc ccgaccagga
     4321 cgtcgccggg gtgccccagg gcctccacct ggcgggcgaa gacgccctcg aacccgaagt
     4381 cgttcgcgac cgccgtcagc accgaggtgt cggtggtcag cgcgagcgcc gggatggccg
     4441 gccggcggtt gtccagcgtg agggtgctca cgaactcggt ggccaggccc tggctgtcgg
     4501 cggcgctccc gccgttgccg cagatcagga tcttgccccc gtccagcacg gcgttcacca
     4561 gcagctcggc ggcggcggcc acgtcctcgg cgcagccctc ggctgcatgg cgcgccgtcc
     4621 gggcggtccc ctccaggtac cctcggaccc aggaggcgtt cacggggccg ctccgttggc
     4681 caccatggat cggatcacct cggccgccgc gggcaggtcg gccaccaccg cgtcggccac
     4741 ctcgccggcc ccgtgctcga gctcctgccg cccgtggccg gtcaggagca ggatcgaggt
     4801 ggcccccagg gcccggccga gctccatgtc cccccggtgg tcgcccacca cgaacgactg
     4861 ggagggatcg aacccgtgct cggccgcggc gcgcagcgcc agacccgtgg ccggcttgcg
     4921 gcacgcgcat cccgcctccg gcccgtgggg gcatgcgtag atcccgtcga tggtggcgcc
     4981 cctccgcatc aactggtcca cgagccgcgc gtggatcgca tccagctcgg tcatcgagat
     5041 ccacccccgc cccaccgggg attggttggt caccacgacg atccccaggc cgaggtctcg
     5101 cagcatgcgg atggcctcgg cggcgccggg gatcaggcgg acctgagccg gatccaggag
     5161 gtggtcgacg tcctcgttga tcgtgccgtc ccggtccagg agcacgaagc gagcgctcat
     5221 cgccgcggcg tccatcgatc cgaaccggtc cgtgcctcga cgcccccctc ggagaagtcg
     5281 aacggcgaga agcgcgcgcc gagggcctcc agctgccggc ggacccggga tcgcacctcg
     5341 ggggggcagg acaggagcag gtacccacca cccccggcgc cgcagatctt gccgccggtg
     5401 gcccccgccg cccgagcccg ggccaacagc tcgtcgatcg cggtcccgtc ggcgatgtgc
     5461 gggttcatcc gccgtttgct ctcgtacgcc tcctggagca ggggccccag cgacccaacg
     5521 tcgcccgcct cgagcacgtc gcgcatctcg taggccatcg cctggagctg cttcatcccc
     5581 tggatcgtct cctcgcgtcc ctcgcggtac aggcggatct gggtgtcgat cagacccaga
     5641 ttggtccgaa cgccgcccgt gtagcagagc agcaggtgcc gccggagcgc gtcgatggtc
     5701 cccccgctca gccggaccga ggtgacggcg acccctgagg cggcgaactc gatcaggttg
     5761 aagccaccga acgcggccgc gtactggtcc tgccagcccc ccgcgatgcc gaggtcgttc
     5821 cgctcgatcg tgtagctcag gtgcgccagc tcgtgagcgg agatctcgat gtcgccgaac
     5881 gcggccagcg cggccaccac tgcggtgacc atggccgagg acccgcccag gccgctaccc
     5941 ggcggagcgt ccgagcggag ctccaggtcg atcccccgat ccagccccag gcgtcggacg
     6001 gccgccttgg gcaggtccag gacaccgtcg aaggtcggcc cctctcgcag gtggtactgg
     6061 gcgtgctgat cgagccgcag gtcgcgcagc ctcacccggg catcgctccg cggcgacagc
     6121 gacacgcgcg cgaagcgatc gatcgtggcc gacaggacgg ccccaccgtg ccgctcgtac
     6181 cagtggggga agtcggtccc cccaccgacg aagctgatcc gcaggggcgc ggcggcccgg
     6241 atctcccgat cgctcatcgg ggctcagtat gggcggcggg cggaccgggt gaccagccgg
     6301 tcgctcaggc cacgaagcgc accaggcggc gtcgctgctg ctcggtgagg cccccccaca
     6361 cgccgtagcg ctcgccgttc ttcagcgccc aggccaggca ggtctcccgg atggggcacc
     6421 cggagcagta ggcaagcgcc ggtcccgcct cctcctccga ggtggggaag aacacgtcgg
     6481 gctcgatccc gtagcaggcg gcgtcctcct gccacatgtc gggtggtgat gggtcacggg
     6541 gaagggtctt gaggtcgatg ccaccgttgg actctctgct caccagctga ctcctgtgct
     6601 gtggccgacg tctgcccgac ccccgggggc cgagctggaa gatgccctat tgatagatcc
     6661 atggcgcagt gaagtgaagt cttgaaaacg ccggacggcc gtagtaggaa tcgagcctca
     6721 cacccgctcc aaccgcaggc cggaggcggt cagcgccccc tggcgtcgct cccaccggac
     6781 cctgcccagg agggcggtgc ccccccggcc gtcgggtgtg gcgctggtca cgaggccggc
     6841 ccgctcgcct gcggcatcca ccgtggcgcc gggggcgacg ggtccgtcgg cgtgcagcgc
     6901 gagcaccact cgcggtgggt ggcccaggtt gcggatcttc gccacggact cctgacccag
     6961 gaagcatccc ttggtggtgt ccacggtctc atccagaccg gcctcggccg gcacggaggt
     7021 ctggtccacg tcgacgggga accggggcag acctcggagg atgcgccagg cctccacggc
     7081 ccgagcggag acctccacgc gatcctcgga caggagccga cgcgccgcgg cgagctcctc
     7141 cccaggcccc aggacgtcga acccatcacc cagcacggac ggggaccaga tcgcggggag
     7201 ctcggccgga acggcccagg gcccagggac ggagagcacg ccaagcggac agggctccag
     7261 tcccacctcg gaggagagca cgtagggctc gagcaggtcg gcgaccggcg cgggctgatc
     7321 ggcgctctgg gcgagcacga acgtcccgtc ctccatgccg tagacgtgga cgtccgcccg
     7381 gatgtgcccg gaaggggtga gcaacaggca ccggcggacc gagaaggcct ccagcccggc
     7441 gatgtccgcg gtgaccagat cgtgcagcca tcgcctcgcg tccgtgccga cgacgctggt
     7501 cagccggaga tccgaccagg ccaccagggc acgcgcgtcg tcgagcgccg agatctgctc
     7561 ttcgagcgtg cgttcgtcca tctccccctg ggaggataag cggtcgtggg gcttccacgg
     7621 accctgctgg tcacgaacga cttcccgccc cgggtgggcg ggatccagcg cacgctgcat
     7681 gccctgtggc gggagctccc ggccgaccgg gtctcggtct tcgccccagc ctcggagggc
     7741 tccccgaggt tcgacggtgg cgagccatac gtgatccggc gcggggaccg cgggttcctg
     7801 tggccgacca cacggacgcg cgatgcgatc cttgccgctg ccggggaggt ggaggcagag
     7861 gtggtgctgt tcggcgcggc gtacccgctg gccctgctcg gtccggccct gtcgagggcc
     7921 ggactccggt acgtgacggc ggcccatggg ttcgagtact ggctgtcgct ggccccgggg
     7981 acccacgcgc tgatgcgccg ggccacctcg cgcgcctgcc acgtggcggt gatgtgcagc
     8041 gccttcatcg cccgcgtggt ccgtaccgcg gtcccggagc gggttcccgt gtcggtgatg
     8101 tacccggggg cggacgtgga ggccttccgc ccggacctgc ccaccgagga cctgcggcgt
     8161 gtccacggcc tgggagcgcg cccggtggtc gtgtgcgtca gcaggctggt cccgcgaaag
     8221 gggcaggacg tgctgatccg ggccatgccc cagatccgcc agcgggtccg ggacgccgcg
     8281 ttgttggtcg tgggggacgg gccccaccgc gccaggctcg agcggctggc cgaggagcac
     8341 gctccgcgct cggtggtctt cgccggacag gtctcggaag ccgatctgcc c
//
LOCUS       DASQQQ010000154        11767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_311703, whole genome shotgun sequence.
ACCESSION   DASQQQ010000154 DASQQQ010000000
VERSION     DASQQQ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11767
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311703"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VE646_06300"
     CDS             <1..606
                     /locus_tag="VE646_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZD79633.1"
                     /translation="AGIDGIVIPGGFGWRGVEGKLEAIRYARENGVPFLGLCLGLQCA
                     VIEFSRHVCGLSGANSSEFDPATPHPVIDLLPEQKNVTDLGGSMRLGAQPCYLVEGTR
                     AAMAYGEPVVEERHRHRYEVNPVYHDVLREHGLVFAGRSKDERLVEIIELPDHPFFVA
                     GQFHPELRSRPTRPHPLFRDFVGAARTHHHDEAERTVVIPG"
     gene            614..1144
                     /locus_tag="VE646_06305"
     CDS             614..1144
                     /locus_tag="VE646_06305"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZD79634.1"
                     /translation="MPDPEDSARAYEGRFVRVDRESWGSSTWEVVRRQGPAAQGAVAV
                     VALTPADRVILVRVFRPPIRTALLEIPAGLLDREDEDALSCARRELREETGYRASKIE
                     FLGGWYSSAGMTDEYVQAFLARTDEEPEGPTDGEVEEVVTLAFEEAVARARAGRVRDA
                     KTALSLLLAAERRSRP"
     gene            1237..2361
                     /gene="ald"
                     /locus_tag="VE646_06310"
     CDS             1237..2361
                     /gene="ald"
                     /locus_tag="VE646_06310"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HZD79635.1"
                     /translation="MIVGVPKEVKDNEYRVALTPEGARELVRAGHRVLVQDGAGAGSS
                     IPQERYQRAGAEIVPGADEVWGSSDLILKVKEPVPEEYGHLRQGQILFTYLHLAAGRE
                     LTRGLVDTKVAAVAYETVQSRDGRLPLLAPMSEVAGRMAPHVAARLLEKEHGGRGILM
                     GGVSGVRPANVLVLGAGMAGSNAAWIAAGMEAAVVVVDKDLDKLRFIDQIHKGRITTL
                     MSDQLTLEQRVREADVLIGSVLVPGARAPKLVTAEMVGSMRPGSVVVDIAIDQGGCVE
                     TSRMTTHSDPTYVEQGVVHYCVGNMPGAVPNTSTYALTNVTLPYALSIAALGLEEAAR
                     RDPALALGVNVYQGQVTNAGVAEAHSLPFTELDRLIDGMR"
     gene            2376..3329
                     /gene="xerD"
                     /locus_tag="VE646_06315"
     CDS             2376..3329
                     /gene="xerD"
                     /locus_tag="VE646_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014360197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HZD79636.1"
                     /translation="MVDAVSAQVERFLDHLWVERGLSDNTVQAYRRDLGRYARWLRTR
                     GCEDASRVTGDDVRAFVAGLSASTHADGHRYRATSVVRALSAVRSFHRFLLREGEATE
                     DPTAGVVRPRLPRALPRPLSVGDVQRLLSAPAEGTSAGLRDRAILEVLYGAGLRVSEL
                     TGLDVDDVDLQEGSVRVLGKGSKEREVPLGGYARDAVAAYLTRVRPDVATPRSRAALF
                     LSLRGGRLSRQACGRLVSDHAKRAGIAGRVTPHSLRHSFATHLLEGGADVRVVQELLG
                     HASVATTQVYTLVTEEHLREVYFTTHPRARRVTSAARRDAG"
     gene            3326..3682
                     /locus_tag="VE646_06320"
     CDS             3326..3682
                     /locus_tag="VE646_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HZD79637.1"
                     /translation="MIGDAQQLQLRGELEQQREHLRREIEEHGGDADSDDAAFDVERG
                     FADSAHSTAERARLLSVLKALRANLRLVNRALTKMELGTYGVCEACGQPIGAERLEAL
                     PWAILCFDCKQKGEGR"
     gene            3705..4871
                     /locus_tag="VE646_06325"
     CDS             3705..4871
                     /locus_tag="VE646_06325"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HZD79638.1"
                     /translation="MPRVLLLVCDSFGVGDAPDAAAYGDEGSDTLGNTSRSVGGIGAP
                     NLERLGLGLLTPIQGVEPTAAPGTAHGRLTERSSGKDTTTGHWEMSGIVLDQPFPLYP
                     EGFPPEVVEPFEREIGRQVLGNRPASGTEIIEELGAEHLATGRPIVYTSGDSVFQIAC
                     HERAVPVSTLYGWCRVARRILTGPHAVGRVIARPFTGEPGSFTRTPRRRDFSLPPPGP
                     TVLDQCSEQGVAVYGVGKIQDIFAGRGLTEARYSESNDHGIDLTLDYLARPAPGLVFT
                     NLVDFDSKYGHRNDPPGYAAAVEALDRRIPELIGALSGGVLLLTGDHGCDPTTPSTDH
                     SRERTPLLASGLPDGPRDVGTRDSFADLGATVADLLGVEVGGLVGRSFVEELGL"
     gene            4993..6312
                     /locus_tag="VE646_06330"
     CDS             4993..6312
                     /locus_tag="VE646_06330"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HZD79639.1"
                     /translation="MTFSDPRDVVAVKRDGGRLSAEDLSLFVQGYARGEVRDELAAAF
                     LMAAFIRGLDREETLSLTRTMIASGRTLPLSGISRPTVDKHSTGGVADGVTLAFAPIA
                     ASLGLAVAKLSGRGLGHTGGTLDKLESIPGLRTDLSPGAFERQVDQIGCAVAAPTRDL
                     VPADGALYALRDATATVPSVPLIAASVMSKKLAVDTDLVLLDVKAGSGAFMKSPEEAR
                     ALAAVCAELAADWGRACRVAVTDMSQPLGQAIGNAIDIEESIRLLRGELPGRLLDLAL
                     TFCSRALAGLTGASPEDARSRAAAAVSSGAALESFRRMVQAQGGDPRVVDDPRGVLPA
                     APVRRPIPAVTAGWLVAVDAEAIGRASGELGAGRKRKGDRIDPAVGIVFEPKVGDRVE
                     KGQRLGTVHARSEDRAQACSARVLEALRFAEEPVEPPPLVFDWFGEP"
     gene            6332..6973
                     /locus_tag="VE646_06335"
     CDS             6332..6973
                     /locus_tag="VE646_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZD79640.1"
                     /translation="MRFALYLLVALAPSLFLHEYAHALTADRLGDPTPRRWGRLSFNP
                     RPLIDPFGTVILPGLLLVLIASGLFVPLFAYTKPFPHDPTYLRNRERDELLVQLSGPL
                     LSLALAVIAGIVLRTTVAGEAGLFIFAVVYVNLLLFVFNLMPIPGLDGARILARFLPP
                     RAREVYRNLDQYLALFMLLVFFLLGGLFLGIVNALSGGLCRAVTGGAGLCGIF"
     gene            7113..7850
                     /locus_tag="VE646_06340"
     CDS             7113..7850
                     /locus_tag="VE646_06340"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HZD79641.1"
                     /translation="MTEDVAPGGFAVDLPVFTGPFKLLSDLLLDQRIDVCDVPVARIT
                     DRYLSYSAQADHWNLEEATWFLAVCAILLELKVSRLMPRHRVPDEEDLLGVSPDLVYA
                     RSLELAAFRRMSLELARRMEEESRYVPRLAGPPPEFAHLYPDVLETVTVDGLTALAVA
                     ALRPAPLLDLYHVTPIRYSMEQAVEAVTGHLGRLGSARFRELVADCEERIHVVVRFLA
                     LLELYREGKVDLEQAETFGEIEVRWQG"
     gene            7858..8415
                     /gene="scpB"
                     /locus_tag="VE646_06345"
     CDS             7858..8415
                     /gene="scpB"
                     /locus_tag="VE646_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010476081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HZD79642.1"
                     /translation="MEERTDRLRALEALLFVSDEPVTSTILAQALSLDRTEVEGLCEE
                     LARSYEERGAGIVLRGVAGGWRLSSHPDVAPIVEGFVLSSRHTRLTKAALETLAIVAY
                     KQPVTRHQVSAIRGVNSDGVLRALAERGLVAEVGREEGPGRPMLYGTSPEFLERLGLP
                     SLSALPPLAPLLTQSAQEAEAAGDE"
     gene            8422..9141
                     /locus_tag="VE646_06350"
     CDS             8422..9141
                     /locus_tag="VE646_06350"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014628938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZD79643.1"
                     /translation="MAEERLQRALARAGLGSRRACEELIRAGVVTVNGRTATLGERVD
                     PSTDVVAVRGHRVNVDPERRYLALHKPAGITTTLGDRHAEADLRSLLPAGPRVVPIGR
                     LDRETEGLLLLTNDGQLAHRLTHPRFGVEKEYLARVDGLPSERQLARLRAGVELEDGP
                     ARAVSIRSVGRSRTSGALRLVLTEGRKREVRRMLSAVGLPVTRLVRLRVGPIRLGRLA
                     PGEVRELTPEECRALFRAAGL"
     gene            9155..9541
                     /gene="aroH"
                     /locus_tag="VE646_06355"
     CDS             9155..9541
                     /gene="aroH"
                     /locus_tag="VE646_06355"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HZD79644.1"
                     /translation="MMGEDRVEGERVRAVRGAICVERDTPKAILDATERLLSQMLRRN
                     DVEEADVVSIFFTATEGLRSTFPAEAARRMGLGRVPLLCATEIPVPGSLPRVVRVLMH
                     LYTSRSRAGILHVYLDGAESLRDDLD"
     gene            9528..10622
                     /locus_tag="VE646_06360"
     CDS             9528..10622
                     /locus_tag="VE646_06360"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HZD79645.1"
                     /translation="MTSTDRVAVIGTGLIGTSIALAAARLGQQVRGFDADRRVLAVAA
                     ERSGLHPTSDAAECARGATLVFLCTPVETIPAVASEVLGAEPEATVTDVASVKSRIMV
                     EVEAAVRPADLPRFVGGHPMGGSERSGPRWAAAGVLDGIVWVLTPTERSSSEAQERLS
                     AWIARVGGKPMIMDPARHDRLVAAVSHLPQVASTALMGLAAAEEAGEPDILLLAAGGF
                     RDLTRLAASNPQLWSQILLANREAVAEAIHLYVKRLSAMRDLVLLGSQREIEATFDEA
                     KAARLALGAKATVRTGVAVLQVPVPDRPGALADLTSALGAAQVNIEDLQIVHSPEGGR
                     GTVHLTVGRTQVEAAVGALGGHGFEPRRLA"
     gene            10660..>11767
                     /locus_tag="VE646_06365"
     CDS             10660..>11767
                     /locus_tag="VE646_06365"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HZD79646.1"
                     /translation="MVEGSVRVPGDKSIAHRWLILAATARGRSWIGDLPPSLDVRSTA
                     RCLALLAPSVRPSLEGLTWKPARGTEPDGFTWDPGAYETWSSQVEVEAEGRDGLRAAS
                     TPLDCGNSGTTMRLLAGVLAGTGFTTTLTGDPSLRTRPMERVAEPLRRMGARVHTEDG
                     HAPLVVEGAALRGIAYRVPLPSAQVKSAVLLAGLAAEGETSVREPAPTRDHTERILEV
                     LGAPVRRSDGAIVVSAYQHGGLRGRVPGDVSSAAFLLAAAALTGGRLSLPRVGLNPTR
                     TRFLEVMRRMGVRVHERIDGDELGEPIGELLVEPGDRPIGTVVTPDELPLVIDEVPVL
                     AALAVHAVGPTRFAGAGELRVKESDRLAGLARMLR"
BASE COUNT         1550 a         3981 c         4444 g         1792 t
ORIGIN      
        1 gccgggatcg acggcatcgt gatccctggc gggttcggat ggcggggcgt cgaaggcaag
       61 ctcgaggcga tccgctacgc ccgcgagaac ggtgtcccct tcctgggcct gtgcctggga
      121 ctgcagtgcg ccgtgatcga gttctcccgc cacgtctgcg gcctgagcgg cgcgaactct
      181 tccgagttcg acccggccac ccctcacccc gtgatcgacc tgcttcctga gcagaagaac
      241 gtgacggatc tggggggctc gatgcgcctg ggtgcgcagc cctgctacct ggtggagggc
      301 acgcgggccg cgatggccta cggggagccg gtggtggagg agcgccatcg ccaccgctac
      361 gaggtcaacc ccgtgtacca cgacgtcctc cgtgagcacg gcctggtgtt cgccgggcgc
      421 tccaaggacg agcggctggt ggagatcatc gagctcccgg atcacccctt cttcgtggcc
      481 gggcagttcc accccgaact gcgatcgcgt cccacccgcc cccacccgct gttccgtgac
      541 ttcgtggggg cggcacggac ccatcaccac gacgaggccg agcgtaccgt cgtgatcccc
      601 ggctgaagcc ccggtgcccg atcccgagga cagcgcacgc gcctacgagg gccggttcgt
      661 gcgcgtggac cgcgagtcgt ggggctcctc cacctgggag gtggttcggc gccaggggcc
      721 ggccgcccag ggggcggtgg cggtcgtggc cctcaccccg gccgaccggg tgatcctggt
      781 ccgggtcttt cggcccccca tcaggaccgc tctgctggag atcccggccg gcctgttgga
      841 ccgcgaggac gaggacgccc tctcgtgcgc gaggcgcgag ctccgtgagg agaccggcta
      901 ccgggcgtcc aagatcgagt tcctgggcgg ctggtactcg agcgcgggca tgacggacga
      961 gtacgtgcag gcgttcctgg cgcggaccga cgaggagccg gagggaccga ccgacggcga
     1021 ggtggaggag gtggtgacgc tcgcgttcga ggaggccgtc gcgcgggccc gcgccggccg
     1081 ggtgcgcgac gcgaagacgg cgctctcgtt gctcctggcc gccgagcgcc ggtcgcgccc
     1141 ctgaccatcc ggcccccggc ggacggtccc atcgaacccc gtccctcctt ttggggtcgg
     1201 gtacgcttgt cggcgcaccg ccggaagggg gcgaacgtga tcgtcggggt cccgaaagag
     1261 gtcaaggaca acgagtaccg cgtggcgctc acgcccgagg gcgcccggga gctcgtccgg
     1321 gccggccatc gtgtcctggt ccaggatggc gccggcgcgg gctcgagcat cccccaggag
     1381 cgataccagc gcgcgggggc cgagatcgtt cccggggccg acgaggtctg gggctccagc
     1441 gacctgatcc tgaaggtcaa ggagcccgtg cccgaggagt acggccacct ccggcagggg
     1501 cagatcctct tcacctacct gcacctcgct gccggcaggg agctcacccg gggcctggtg
     1561 gacacgaagg tcgcggcggt ggcctacgag acggtgcagt cacgggacgg gcggttgccg
     1621 ctcctcgccc ccatgagcga ggtggccggg cggatggcgc ctcatgtcgc ggcccggttg
     1681 ctggagaagg agcacggtgg tcggggcatc ctgatgggcg gggtgtccgg cgtccgtccg
     1741 gccaacgtgc tcgtgctcgg tgcggggatg gccggctcca acgccgcctg gatcgcggcc
     1801 gggatggagg cggccgtggt ggtggtggac aaggacctgg acaagctgcg gttcatcgac
     1861 cagatccaca agggccggat caccacgctg atgtccgacc agctcaccct cgagcagcgg
     1921 gtccgggaag ccgacgtcct gatcggatcg gtcctggtgc cgggtgcgcg cgcgccgaag
     1981 ctcgtcacgg ccgagatggt gggctcgatg cgtccgggct cggtggtcgt cgacatcgcc
     2041 atcgaccagg gtggctgcgt ggagacctcc cggatgacga cgcactcgga tccgacctac
     2101 gtcgagcagg gtgtggtcca ctactgcgtt gggaacatgc ccggcgcggt ccccaacacc
     2161 tcgacgtacg cactgacgaa cgtgaccctc ccctatgcgc tctccatcgc ggcactcggc
     2221 ctggaggagg cggcgcgccg ggatccggca ctggccctgg gggtgaacgt ctaccagggg
     2281 caggtgacca acgccggcgt cgccgaggcg cactccctgc ccttcacgga gctcgaccgg
     2341 ctgatcgacg gcatgcgctg agcgggacgg gacggatggt cgacgccgtg tcggcacagg
     2401 tcgagcggtt cctggatcac ctgtgggtgg agcgcggact ctctgacaac accgtgcagg
     2461 cctaccgtcg ggacctcggc cgctacgcgc gatggctccg gacccgggga tgcgaggacg
     2521 cgtcgagggt caccggcgac gacgtgaggg cgttcgtggc cggcctctcc gcgtccacgc
     2581 acgctgacgg ccaccggtac cgcgccacct ccgtggtgcg agcgttgtcg gcggtccggt
     2641 cgttccatcg gttcctgctc cgggagggcg aggcgaccga ggacccgacg gccggcgtgg
     2701 tgcgaccgag gctgccccgt gcgctgccca ggcccctctc cgtcggcgac gtccagcggc
     2761 tgctgtccgc gcctgccgag ggaaccagcg ccggactgcg cgaccgggcc atcctcgagg
     2821 tcctctacgg tgcggggctc cgggtctctg agctcacggg gttggatgtg gacgacgtgg
     2881 atctccagga ggggagcgtc cgggtcctgg gcaagggcag caaggagcgg gaggtccccc
     2941 tgggcggcta cgcccgagac gcggtggccg cctacctcac ccgggtgcgc cccgatgtgg
     3001 ccacgccgcg gagcagggcg gcgctcttcc tgagcctgcg ggggggccgc ctgagccgcc
     3061 aggcctgcgg ccggttggtc tcggaccacg cgaagcgcgc cgggatcgcc gggcgggtga
     3121 ccccccactc gctgcggcac tcgttcgcca cgcacctgtt ggaggggggt gcggacgtgc
     3181 gggtggtcca ggagctgctg ggacacgcga gcgtggccac gacccaggtc tatacgctgg
     3241 tgaccgagga gcaccttcgc gaggtgtact tcacgacgca tccgcgggcg cggcgcgtga
     3301 cgtccgcggc gaggagggat gccgggtgat cggagatgcg caacagctgc agctccgggg
     3361 agagctcgag cagcagcggg agcacctacg cagggagatc gaggagcacg gtggcgacgc
     3421 cgattcggac gatgccgcgt tcgacgtgga gcgggggttc gcggactcgg cgcacagcac
     3481 cgctgagcgc gcccgcctgc tctccgtcct caaggcgctg cgggccaacc tccgcctggt
     3541 gaaccgggcc ctcacgaaga tggagctggg gacctacggc gtgtgcgaag cgtgcgggca
     3601 gccgatcgga gccgagcggc tcgaggcgct gccgtgggcg atcctctgct tcgactgcaa
     3661 gcagaagggc gagggccggt gacgtcgacg tgaatccgtc ccgggtgcct cgcgtgctcc
     3721 tcctggtctg cgattccttc ggggtgggtg atgctccgga cgcggccgcg tacggggacg
     3781 agggctcgga cacgctcggg aacacctcgc gcagcgtcgg agggatcggg gcgccgaacc
     3841 tggagcgcct cggtctaggt ctgctcacgc cgatccaggg ggtggagccc acggcggctc
     3901 cgggcacggc tcacggacgg ctgacggagc gctcgtccgg caaggacacc accacggggc
     3961 actgggagat gagcgggatc gtgctcgacc agcccttccc cctgtacccg gaggggttcc
     4021 caccggaggt ggtcgagccg ttcgagcggg agatcggccg gcaggtgctg gggaaccggc
     4081 ccgcgtcggg caccgagatc atcgaggagc tcggggccga gcacctggcc accggcaggc
     4141 cgatcgtgta cacgagcggc gactccgtgt tccagatcgc ctgccacgag cgtgcggtgc
     4201 cggtgtcgac gctgtacggg tggtgccgag tggcccgccg gatcctcacc gggccgcacg
     4261 ccgtggggcg ggtgatcgcc cgacccttca cgggggagcc gggctcgttc acccggaccc
     4321 cccggcggcg ggacttctcg ctgccgccgc ccggtcccac ggtgctcgac cagtgcagcg
     4381 aacagggggt ggccgtgtac ggcgtgggga agatccagga catcttcgcc gggcggggcc
     4441 tgaccgaggc caggtactcg gagtccaacg atcacggcat cgacctcacg ctggactacc
     4501 tggctcggcc cgccccgggc ttggtcttca ccaacctggt cgacttcgac agcaagtacg
     4561 ggcaccgcaa cgacccgccc ggatacgcgg ccgccgtcga ggctctcgat cggcggatcc
     4621 ccgagctcat cggtgccctg agcggaggcg tgctcctgct gacgggcgat cacggctgcg
     4681 atccgacgac cccctccacg gatcactctc gcgagcggac cccgctgctg gcgtccggcc
     4741 tgcccgacgg gccgcgggac gtgggcactc gggacagctt cgcggacctg ggcgccacgg
     4801 tggcggacct gctgggggtc gaggtcgggg gactggtcgg gcgcagcttc gtggaggagc
     4861 tcggcctgtg agcctccggg atcgagctgc acctccggac ggtggagcgt gccctcagcg
     4921 aggtgggatg gaccatcgag ctgaagtgga ggtcgaccat cgtcgggacg accggaggag
     4981 catgaggtcc aggtgacgtt ctccgacccc cgggacgtgg tcgcggtcaa gcgggacggc
     5041 ggccgtctgt ccgcagagga cctttccctg ttcgtgcagg gctacgcgag gggcgaggtg
     5101 cgggacgagc tcgcggcggc gttcctgatg gctgccttca tccgggggct ggaccgggag
     5161 gagaccctgt ccctcacgcg gacgatgatc gcctcgggcc ggaccctgcc gctctcgggc
     5221 atctcccggc ccacggtgga caagcactcc accgggggcg tggccgacgg cgtcaccctg
     5281 gcgttcgcgc cgatcgcggc gtccctgggc ctggcggtgg cgaagctgtc cggccgcggt
     5341 ctgggccata ccggggggac cctcgacaag ctggagtcga tcccgggcct tcggaccgat
     5401 ctcagtccgg gggcgttcga acggcaggtg gaccagatcg gctgcgccgt ggcggccccg
     5461 acgcgcgacc tcgtgccggc cgacggcgcg ctgtacgccc tgcgggacgc gacggccacg
     5521 gttccctccg tcccgctgat cgccgccagc gtgatgtcca agaagctggc cgtcgacacc
     5581 gacctcgtgc tcctggacgt gaaggcgggg agcggcgcat tcatgaagag cccggaggag
     5641 gctcgagccc tggccgccgt gtgcgccgag ctcgcggccg actggggccg ggcatgccgg
     5701 gtggccgtga cggacatgtc gcagcccctc ggccaggcga tcgggaacgc gatcgacatc
     5761 gaggagtcga tccggctgct ccggggtgag ctccccggcc ggctcctgga cctggccctc
     5821 accttctgct cgagggccct ggccgggctg accggcgcct cgccggagga tgctcggagc
     5881 cgggccgccg cagcggtctc gtccggcgcg gcgctcgagt ccttccgccg catggtgcag
     5941 gcccagggag gcgatccccg tgtggtggac gatccgagag gcgtccttcc cgcggccccg
     6001 gtccgtcggc ccatcccggc cgtcaccgcc ggttggctgg tggccgtgga cgccgaagcc
     6061 atcggacgag ccagcgggga gctcggcgcg ggacgcaagc gcaaagggga tcggatcgac
     6121 cccgcggtcg gtatcgtgtt cgaacccaag gtgggggacc gggtggagaa ggggcagcgg
     6181 ctgggcacgg tgcacgcgcg gagcgaggat cgggcccagg cgtgctcggc cagggtgctc
     6241 gaggccctga ggttcgccga ggagcccgtc gagccgccac cgttggtgtt cgactggttc
     6301 ggtgagccct gacgggggtc cgaggatgaa cgtgcgcttc gcgctgtacc tgctggtcgc
     6361 gctcgcgccc agcctgttcc tgcacgagta cgcgcacgcc ctcaccgccg accggttggg
     6421 cgatcccacc ccccggcggt ggggtcgact gtcgttcaac ccgcggccgc tgatcgatcc
     6481 cttcggcacg gtgatcctcc cggggctctt gctggtcctg atcgcctccg gtctgttcgt
     6541 gccgctgttc gcctacacga agcccttccc ccacgatccg acctacctgc gcaaccggga
     6601 gcgcgacgag ctgctcgtgc agctgtcggg tccgctgctc agcctggccc tggcggtgat
     6661 cgcggggatc gttctccgca ccacggtggc cggggaggcc ggcctgttca tcttcgccgt
     6721 ggtctacgtc aacctcctcc tgttcgtgtt caacctcatg ccgatccccg gcttggacgg
     6781 agcccggatc ctggccaggt tcctgcctcc acgtgcccgg gaggtctacc gcaacctcga
     6841 ccagtacctg gcgctgttca tgctcctggt gttcttcctg ctcggtggtc tgttcctggg
     6901 catcgtcaac gcgctgtccg gtggtctgtg tcgcgccgtg accggtgggg cgggcctgtg
     6961 cggcatcttc tgatggatct cccggaggcc cgagccgccc gcgatatcat cgcgtccatc
     7021 gtggcgggag catcgatccg tggtcgcggg gccctggagc ccgatccgac cgggtgcggc
     7081 gggaccggag tggcggccga accgggacca tcgtgaccga ggacgtcgct cccggcgggt
     7141 tcgccgtcga cctgcccgtg ttcacgggcc cgttcaagct cttgagcgac ctcctgctcg
     7201 atcagcggat cgacgtctgc gatgtcccgg tcgcccggat cacggatcgg tacctctcct
     7261 actcggcgca ggccgaccac tggaacctcg aggaagcgac ctggttcctg gccgtctgcg
     7321 ccatcctgct ggagctgaag gtgagccggc tcatgcccag gcatcgggtg ccggacgagg
     7381 aggatctgtt gggcgtctcg cccgacctgg tctatgcgcg cagcctggag ctggcggcgt
     7441 tccgccggat gtccttggag ctggctcggc ggatggagga ggagtcgcgc tacgtcccac
     7501 ggctggccgg ccccccgcca gagttcgcac acctgtaccc cgatgtcctg gagacggtga
     7561 ccgtggacgg gttgaccgcg ctcgcggtcg cggccctccg gcccgctccg ctgctcgacc
     7621 tctaccacgt caccccgatc cgctactcga tggagcaggc ggtggaggcg gtcaccgggc
     7681 atctgggcag gctcggttcg gccaggttcc gagagctcgt ggccgattgc gaggagcgga
     7741 tccacgtggt ggtccggttc ctcgcgttgc tcgagctcta ccgtgaaggc aaggtcgacc
     7801 tcgagcaagc ggagacgttc ggcgagatcg aggtccgttg gcaaggatga ggtgaaggtg
     7861 gaggagcgca cggatcgtct acgggccctc gaggccctgc tgttcgtctc ggacgagccg
     7921 gtgacctcga ccatcctggc ccaggccctc tcgctggacc gcacggaggt ggagggtctc
     7981 tgtgaggagc tggctcgatc ctacgaggag cgcggcgccg ggatcgtgct gcgcggcgtg
     8041 gccggcggct ggcggctgag cagccatccc gatgtggccc cgatcgtgga gggattcgtc
     8101 ctgtcctccc gtcatacgag gctcacgaag gcggccctgg agaccctggc gatcgtcgcg
     8161 tacaagcaac ccgtgacccg ccaccaggtc tccgccatcc gaggggtgaa ctcggacggc
     8221 gtgctccgag ccctggcgga gcgggggctg gtcgcggagg tgggtcgcga ggagggaccg
     8281 ggccggccca tgctgtacgg cacctctccc gagttcctgg agcgcttggg cctgccttcg
     8341 ctctcggcgc tgccgcccct ggccccgctg ctgacccaga gcgctcagga ggcggaggcg
     8401 gccggggacg agtgagcccg ggtggccgag gagcgtctgc agcgagcgct ggcccgagcc
     8461 gggctcgggt cgcggcgggc ctgtgaggag ctgatccggg ccggggtcgt gaccgtgaac
     8521 ggccggacgg ccacgctcgg cgagcgggtg gatccctcca ccgacgtggt ggccgttcgc
     8581 ggtcaccgcg tcaacgtgga tcccgagcgc cggtacctcg ccctgcacaa gcccgcgggg
     8641 atcaccacca ccttggggga ccggcacgcg gaggcggacc tccgctccct gttgccggcc
     8701 gggccgcggg tggtgccgat cggcaggctc gatcgtgaga cggagggcct gctgctgctc
     8761 acgaacgacg ggcagctcgc ccaccggctg acccacccgc ggttcggggt ggagaaggag
     8821 tacctggccc gcgtggacgg cctgccgagc gagcgacagc tggctcgcct ccgggccggg
     8881 gtcgagctcg aggacggacc ggctcgggcc gtgtcgatcc ggtcggtggg ccgttcccgc
     8941 acctccggtg ccctccggct ggtgctgacg gagggccgca aacgcgaggt ccgccggatg
     9001 ctctcggcgg tggggcttcc cgtgacccgt ctggtgcgcc tgcgcgtcgg gcccatccgc
     9061 ctgggccgcc tggcccccgg agaggtgcgg gagctcacgc cggaggagtg ccgagcgctg
     9121 ttccgggccg ccggactgta gccttttggc gcccatgatg ggggaggatc gggtggaggg
     9181 ggagcgggtg cgggcggtgc ggggggctat ctgcgtggag cgcgacacgc cgaaggcgat
     9241 cctcgatgct accgagcgtc tgctgagcca gatgctgcgc cgcaacgatg tggaagaagc
     9301 agacgtcgtc agcatcttct tcaccgcgac cgaggggctc cggtcgacgt tccccgccga
     9361 ggccgcccgc cgcatggggc tgggcagggt gccactcctg tgtgccaccg agatcccggt
     9421 ccccggatcg ctgccccggg tggtgcgggt gctgatgcat ctgtacacgt cgcggtccag
     9481 ggccgggatc ctgcacgtct acctggacgg tgcggagtcg cttcgggatg acctcgactg
     9541 accgggtggc ggtgatcggc accgggctga tcggcacgtc gatcgccctg gcggcggccc
     9601 gtctgggcca gcaggtgcga gggttcgacg ccgaccgccg ggtcctggcc gtggccgccg
     9661 agcgtagcgg gctgcatccg acctcggatg ccgccgagtg cgcacgcggc gccacgctgg
     9721 tcttcctgtg cacgccggtc gagacgatcc ctgcggtggc gtccgaggtc ctcggcgccg
     9781 agccggaggc cacggtgacc gacgtggcga gcgtgaagtc tcggatcatg gtcgaggtcg
     9841 aggcggcggt ccgcccggcg gatctcccgc gctttgtggg cggccacccg atgggcggct
     9901 ccgagcggtc cgggccccgc tgggcggccg cgggggtgct ggacggcatc gtctgggtgc
     9961 tcacgcccac cgagcgttcg tcctccgagg cacaggaacg tctgagcgcc tggatcgccc
    10021 gggtaggcgg gaagccgatg atcatggacc cggcccggca cgaccgattg gtcgccgcgg
    10081 tcagccacct gcctcaggtg gcctccaccg cgctgatggg cctggccgcg gcggaggagg
    10141 cgggcgagcc cgacatcctc ctgctggccg caggcgggtt ccgcgacctg acgagattgg
    10201 cggcatcgaa ccctcagctg tggagccaga tcctgctcgc caaccgtgaa gcggtggcgg
    10261 aggcgatcca cctgtacgtg aagcggctct cggccatgcg ggacctggtc ctcctgggct
    10321 cgcagcggga gatcgaggcc accttcgacg aggcgaaggc ggctcggttg gcgctcgggg
    10381 ccaaggccac cgtgcggacc ggcgtggccg tgttacaggt cccggtcccc gaccgcccgg
    10441 gcgctctggc cgatctcacc agtgccctgg gcgctgcgca ggtgaacatc gaggatctgc
    10501 agatcgtcca ttcgcccgag ggcggtcgcg gcacggtgca cctcacggtg gggcggaccc
    10561 aggtggaggc ggcggtcggt gcgctcgggg gtcacggctt cgagccccgg cgactggcct
    10621 gaggaggcat gggtggcggc gaaggtgtct cccggcgctg tggtcgaggg gagcgtccgg
    10681 gttcctgggg acaagtccat cgcgcatcgg tggctgatcc tggccgcgac cgcgcgaggg
    10741 cgctcctgga tcggtgacct tccaccttcg ctcgatgtcc gttccacggc tcgctgcctg
    10801 gcgctgctcg ccccctcggt tagaccttcg ctggagggtc tgacctggaa gcctgctcgg
    10861 ggcacagaac ctgacggttt cacgtgggac ccaggagcgt acgagacctg gagctcccag
    10921 gttgaggtcg aggctgaagg ccgggatggt cttcgagcag cgtccacgcc actcgattgc
    10981 ggcaactcgg ggaccacgat gcgcttgctc gccggcgtcc tcgccggaac ggggttcacc
    11041 accaccctga ccggagaccc gagcctgcgg acccgcccca tggaacgggt ggccgagccg
    11101 ctcaggagga tgggagcgag ggttcacacc gaggacgggc atgctccact ggtggtggag
    11161 ggggccgcgc tgcgaggcat cgcctaccgc gtccccctcc cgagcgcgca ggtgaagagc
    11221 gccgtgctcc tggccggatt ggcggcagag ggagagacct cggtccggga gccggcgccc
    11281 acccgcgatc acacggagcg gatcctcgag gtcctgggcg cgcccgtccg ccgttcggac
    11341 ggagccatcg tcgtctccgc ctaccagcac gggggcttgc gagggcgtgt gcccggcgac
    11401 gtctcctcgg ccgcgttcct cctggccgcg gccgccctga ccggtggacg gttgtccctg
    11461 ccccgtgtgg gcttgaaccc gacccgcacg cggttcctcg aggtgatgcg gaggatgggg
    11521 gtgagggtgc acgagcgtat cgacggcgat gagctgggcg agccgatcgg tgagctcctg
    11581 gtggagcccg gggatcggcc gatcgggacg gtcgtgaccc cggacgagct gcccttggtg
    11641 atcgacgagg tcccggtgct cgcggcgctc gccgtgcacg cggtgggccc gacccggttc
    11701 gcgggagccg gcgagctccg ggtgaaggag agcgatcggc tggcggggct ggcgcgcatg
    11761 ctccggg
//
LOCUS       DASQQQ010000155         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_311726, whole genome shotgun sequence.
ACCESSION   DASQQQ010000155 DASQQQ010000000
VERSION     DASQQQ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311726"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1555
                     /locus_tag="VE646_06370"
     CDS             <1..1555
                     /locus_tag="VE646_06370"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZD79647.1"
                     /translation="YGRLLAGATARRGTTAAVALVALLASLALVPFLGTEFVPALEEG
                     SLLLRVTYAPSTSLNQVIRTTDRMQRDILDAFGDREVRGAVSYIGRAEAGGEPEPVNN
                     VEMTLDLVPEGEWRFGSKTALIAAMDSVVGVYPGVQLTFSQPIEARVDELVSGVRAQL
                     AIKLFGDDLDVLLDRADAIAAVVGDIEGAEDVQVEQVTGQPATEVSVDRGAIARYGLN
                     VEDVLGVIRVGVGGEVVGQLYDGPRRFDILLRLEEPWRDDASRIADLLVPTPSGTNVP
                     VAQLASVRTVVGPKQIGHEGSQRRIVIQANVRGRDLGRFVAEARQAVARRVRLPPGYT
                     VAWSGQFENQQRAMRRLSLIVPLTIALIYLLLFLSLGSLRLAALIILNVPFALIGGIV
                     ALFVTGQYLSVPASVGFIALFGVAVLNGVVLVTYIKQLRDDGAAVDEAARQGAELRLR
                     PVLMTALVASLGLVPLLFSRGAGSDVQRPLATAVVGGLITSTLLTLVLLPSIYRWFDP
                     GAGGEGAAG"
     gene            1552..>2158
                     /locus_tag="VE646_06375"
     CDS             1552..>2158
                     /locus_tag="VE646_06375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZD79648.1"
                     /translation="MSLRARVRRHPLVAFVVLAYLLSWSYWIPLAWNGAVVVPGGSVT
                     HLPGLLGPALAALLVPLLAGDREALADLLRRMRLVTRPVWRFGLVSLSPLVFLGLALP
                     VAAVTGAPLPDVSDFARYSGLPVLELWAVVPIVFVVNGLGEETGWRGFALPRLQQRFG
                     PLGGTLVLAVIWAAWHVPLFQAVETYRLMTPATMLFGFGLGI"
BASE COUNT          242 a          779 c          787 g          350 t
ORIGIN      
        1 ctacggcagg ctcctcgccg gggcgacggc ccggcggggc acgacggccg ccgtcgccct
       61 ggtggcgctc ctggccagcc tggccctggt gccgtttctc ggcaccgagt tcgtcccggc
      121 cctggaagag ggctcgctcc tgctccgggt cacctacgcc cccagcacgt cgctcaacca
      181 ggtgatccgt accacggacc gcatgcagcg ggacatcctg gacgcgttcg gcgaccggga
      241 ggtgcgcggg gcggtctcct acatcggccg cgccgaggcc ggaggcgagc cggagccggt
      301 gaacaacgtc gagatgaccc tggacctggt gccggagggt gagtggcgct ttggctcgaa
      361 gaccgccctg atcgccgcca tggacagcgt ggtgggggtg taccccggcg tgcagctcac
      421 cttctcgcag cccatcgagg cccgggtcga cgagctggtg agcggcgtca gggcccagct
      481 cgccatcaag ctcttcggag acgacctgga cgtcctcctc gaccgggccg acgccatcgc
      541 cgccgtggtg ggcgacatcg agggcgcgga ggacgtccag gtggaacagg tcaccggaca
      601 gcccgccacg gaggtgtcgg tggaccgggg ggccatcgcc cgctacgggc tgaacgtcga
      661 ggacgtcctg ggggtgatcc gggtgggggt ggggggcgag gtggtcggcc agctctacga
      721 cggcccccgg cgcttcgaca tcctcctgcg gctggaggag ccctggcgcg acgatgcgtc
      781 caggatcgcg gacctgctgg tgccgacgcc ctccgggacg aacgtgccgg tggcgcagct
      841 cgccagcgtg cgcacggtgg tgggacccaa gcagatcggc cacgagggga gccagcgccg
      901 catcgtgatc caggcgaacg tgcgcggtcg ggacctgggc cgcttcgtgg ccgaagcgcg
      961 gcaggcggtg gcccggcgcg tcaggctgcc gccgggctac accgtggcgt ggagcggcca
     1021 gttcgagaac cagcagcggg ccatgcggcg cctgtcgctg atcgtgcccc tgaccatcgc
     1081 cctcatctac ctgctgctct tcctgagcct ggggtccctc cggctggccg cgctcatcat
     1141 cctcaacgtg cccttcgccc tcatcggcgg catcgtggcc ctcttcgtga ccggccagta
     1201 cctgtccgtg cccgcctccg tggggttcat cgccctcttc ggcgtggcgg tgctgaacgg
     1261 cgtggtgctg gtcacctaca tcaagcagct ccgggacgac ggggcggccg tcgacgaggc
     1321 cgcacggcag ggggccgagc tccgcctccg acccgtgctc atgaccgcgc tggtggcgag
     1381 cctggggctg gtaccgctcc tgttctcccg gggcgccggc tccgacgtcc agcggcccct
     1441 ggccaccgcc gtggtgggcg gcctcatcac ctcgacgctg ctcacgctgg tgctgctgcc
     1501 cagcatctat cggtggttcg acccgggggc cggcggcgag ggagcggcag ggtgagcctg
     1561 cgggcccggg tgcgccggca ccccctggtc gctttcgtcg tgctggccta cctcctgtcc
     1621 tggagctact ggattcccct cgcgtggaac ggcgccgtgg tggtgcccgg cgggtccgtc
     1681 acccaccttc cgggactgct gggccctgcc ctggcggcgc tcctcgttcc cctgctggcg
     1741 ggcgaccgcg aggcgctggc cgacctgctc cgccgcatgc ggctcgtgac gagaccggtg
     1801 tggcggttcg gcctcgtgag cctgagcccc ctcgtgttcc tgggcctggc gctcccggtg
     1861 gccgccgtca ccggcgcccc cctcccggac gtctccgact tcgcccgcta ctccggcctg
     1921 ccggtgctgg agctctgggc ggtggttccc atcgtgttcg tggtcaacgg cctcggggag
     1981 gagaccggct ggcgcggctt cgcactgcct cgcctgcagc agaggttcgg acccctcggg
     2041 ggcacgctcg tcctcgccgt gatctgggcc gcctggcacg tgcccctgtt ccaggccgtc
     2101 gagacgtacc gcctcatgac gccggcgacg atgttgttcg ggttcggcct cggcatcg
//
LOCUS       DASQQQ010000156         2147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_342766, whole genome shotgun sequence.
ACCESSION   DASQQQ010000156 DASQQQ010000000
VERSION     DASQQQ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342766"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /gene="arcC"
                     /locus_tag="VE646_06380"
     CDS             <1..826
                     /gene="arcC"
                     /locus_tag="VE646_06380"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HZD79649.1"
                     /translation="RQGWEVAITHGNGPQVGFILRRSELASHELHEVPLDVCGADSQG
                     AIGYALQQNLYNDFARLGIHKPVTTVITQTEVEPDDPAFQRPTKPIGSYMDQEQAVSR
                     RTHDGWSVIEDAGRGWCRVVPSPLPGRIVEEDVIRTLLAQGIVVISAGGGGIPVVTDA
                     EGNLRGVAAVIDKDLASSLLASRLGAELLLISTSVEKVALRFGTPEQEWVSELPASAA
                     RSYLEEGGHFAKGSMAPKIQAAVSYVEATGGTAIITDPDNVERALDGDTGTRIVPD"
     gene            920..1465
                     /locus_tag="VE646_06385"
     CDS             920..1465
                     /locus_tag="VE646_06385"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HZD79650.1"
                     /translation="MSRGAIGSDRRSVGAGSLREIIMGAQDNLTTVLAVVLGVSIGSG
                     ELKAIALAGMAAGVAEAISMGGVLYTATRAERDLERNGDGPPSSGPLKDPVVAASVTF
                     AAAIVAAAIPLSPFAVLPVRWATVTAAGLSVTCLFALGWWAGGVTGQHPFRGGLRFVA
                     IGGFAALAAALVGTVLRTNGS"
     gene            1589..>2147
                     /locus_tag="VE646_06390"
     CDS             1589..>2147
                     /locus_tag="VE646_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HZD79651.1"
                     /translation="MDESTVRRIELPQIADPGPLGLAAFALTTFLLSAKNAGWTDGGN
                     AWLGYALAYGGLAQLLAGMWEFRNRNVFGATAFATYGAFWIGLGLYGLLVEGASPNPL
                     NDLGWIVLAFAIFNTYMALWSTRVNVAVFLVFFTLEVTEIVLAIGFLSQTDVIVKAGG
                     VIGVITAVVAWYTSAAGVVNGMAGHP"
BASE COUNT          301 a          742 c          788 g          316 t
ORIGIN      
        1 ccgccagggg tgggaggtgg ccatcacgca cggcaacggt ccccaggtgg gcttcatcct
       61 ccgccgatcg gagctcgcct cgcacgagct gcatgaggta cccctggacg tctgcggcgc
      121 cgacagccag ggcgcgatcg gttacgcgct gcagcagaac ctctacaacg acttcgctcg
      181 tctcggcatc cacaagcccg tgaccaccgt gatcacccag accgaggtcg aacccgacga
      241 cccggccttc cagcgcccca ccaaaccgat cggctcctac atggaccagg agcaggcggt
      301 cagccgtcgg acgcacgatg ggtggagcgt gatcgaggac gcggggcggg gctggtgccg
      361 cgtggtccca tccccgctgc ccgggcggat cgtggaggag gacgtgatcc ggaccctcct
      421 cgctcagggc atcgtggtga tcagcgctgg cggaggaggg atccctgtgg tgaccgacgc
      481 cgagggcaac ctgcggggcg tggccgcggt gatcgacaag gacctcgcgt cctccctgct
      541 ggcctcgcgg ctgggggccg agctgctcct gatcagcacc tccgtggaga aggtcgcgtt
      601 gcgcttcggg accccggagc aggaatgggt ctcggagctg cccgcatcgg ccgctcgctc
      661 ctacctcgag gaggggggac acttcgcgaa aggcagcatg gcccccaaga tccaggcggc
      721 cgtgtcctac gtggaggcga cgggcggaac cgcgatcatc accgacccgg acaacgtgga
      781 gcgcgccctg gacggggaca cgggcaccag gatcgtgccc gactgatccg gcccccccgg
      841 atccgacggc ccgcccgacc cccgaggccc cggcccttcg gcccggggcc gaccgatctg
      901 ggcgggggac gctggatgcg tgagccgggg agccatcggg tcggaccgac ggagcgtggg
      961 ggccggcagc ctccgagaga tcatcatggg cgcccaggac aacctcacga ccgtcctggc
     1021 cgtggtgctg ggcgtgtcga tcgggtccgg cgagctaaag gccatcgccc tggccggcat
     1081 ggccgccggg gtcgccgagg cgatctccat gggtggggtt ctctacacgg ccacccgcgc
     1141 cgagcgcgac ctggagcgca acggtgacgg gccaccctcc tccggcccgt tgaaggaccc
     1201 tgtcgtcgcg gccagcgtga cgttcgcggc cgccatcgtg gccgccgcca tccctctctc
     1261 ccctttcgcc gtgctcccgg tccggtgggc tacggtcacg gccgccggcc tgtcggtgac
     1321 gtgcctgttc gccctggggt ggtgggcggg gggcgtgacc ggccagcacc cgttccgcgg
     1381 gggccttcga ttcgtggcga tcggcgggtt cgcggccctg gccgcggccc tggtgggaac
     1441 cgtgctgcgc acgaacggct cctgacgcgg gggcccaggc ggccggccgg cgcagaacgg
     1501 ccctgcggcg acgggacctt cgacgggtga gccggccacg ctccggcggc agcatcgcag
     1561 ggacgtcgct ggaggaagga aggatcacat ggacgagtcc acggtgcgtc gcatcgagct
     1621 cccccagatc gccgaccctg ggcccctcgg gctggcggcc ttcgccctca ccacgttcct
     1681 cctgtcggcc aagaacgccg gctggacgga tgggggcaac gcgtggctgg gctacgcgct
     1741 cgcctacggc gggctggccc agctgctggc cgggatgtgg gagttccgca accgcaacgt
     1801 cttcggcgcc accgcgttcg cgacgtacgg ggccttctgg atcgggctgg gcctgtacgg
     1861 gctgctggtc gagggggcga gcccgaaccc cctgaacgac ctggggtgga tcgtgcttgc
     1921 gttcgcgatc ttcaacacct acatggcgct gtggagcacc cgggtgaacg tcgcggtgtt
     1981 cctcgtgttc ttcacactcg aagtgaccga gatcgtcctg gcgatcggct tcctgagcca
     2041 gacggacgtg atcgtgaagg ccggaggcgt gatcggggtc atcaccgcgg tggtggcctg
     2101 gtacacctcg gccgcgggcg tggtcaacgg gatggccggg cacccgg
//
LOCUS       DASQQQ010000157         2634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_311766, whole genome shotgun sequence.
ACCESSION   DASQQQ010000157 DASQQQ010000000
VERSION     DASQQQ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311766"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VE646_06395"
     CDS             <1..745
                     /locus_tag="VE646_06395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZD79652.1"
                     /translation="EELHQRDIERAQQLATVGELASGVAHEIKNPLVGIANGLDLVMR
                     RVGADPGLSPIADEMSRQLERIQGAIRDLLAYARPSDPTRAPTEVNRVAKRAATLVGP
                     AAERRGVHVRTELNPAVSEIPADEELIRQALVNLLMNAVQASPPTKEVGVRTVTAPEG
                     AAIEVWDRGRGITPRDLEHIFKPFYTTRHSGTGLGLSITREIVERHGGRLEVESEVGK
                     GSTFRIILPREVPAAHGAGGGSKGEVGSQ"
     gene            742..2118
                     /locus_tag="VE646_06400"
     CDS             742..2118
                     /locus_tag="VE646_06400"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZD79653.1"
                     /translation="MSEERVLVCDDEMLIRMWLEEHLREAGYAVEGTGDGAELLAAME
                     REPADLVLLDLRLPDGSGIDVLERLKQREPGVPVIMITAYGEVETAVAAVRAGAHHFL
                     EKPIQLSELLVLIEQALETRALRGELDRYRDGYRWQFSGVTLVGRSHALRKVAELITR
                     LGMKGSPSNVLIRGESGTGKDVVARALHARGPRRSRPFISVNCTALPESLVESELFGH
                     EAGAYTDARETKRGLLELANGGTIFLDEIGDMPKAAQSKLLGFLEHRSFRRVGGVRDV
                     EVDVHVIAATNRDLEAAVAGGSFREDLFYRLNVVPIWLPPLRERPEDVAPLVLHLVET
                     LCGDLRQPVREVEPGAMRALERHAWPGNVRQLRNILERVLLLHDDGPIHEADLPAEIR
                     GGREEADGRLVVLPPSGISLDELERELIAQALERTDGNKTGAARLLGVSRDTLRYRLE
                     KYGMDGPE"
     gene            complement(2127..>2634)
                     /locus_tag="VE646_06405"
     CDS             complement(2127..>2634)
                     /locus_tag="VE646_06405"
                     /inference="COORDINATES: protein motif:HMM:NF016334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HZD79654.1"
                     /translation="PDWLAAGYRVLLDRDRIADRLPPPSVLAYVPRPPSDAEYRAVVE
                     EFWWESLYVAKYIGRGELLPARYSLETVLRYRCLVPMLEWYVQIGRGWEQSVGVRGSG
                     LRWLLAPDERTALDATYAGDTLRGHAEALEAMVELFTHAARAIARDLGYTYPSTVDRE
                     TRMLLRRD"
BASE COUNT          408 a          824 c         1050 g          352 t
ORIGIN      
        1 ggaagagctg caccagcgag acatcgagcg ggcgcagcaa ctggcgaccg tgggggagct
       61 ggcgagtggg gtggcgcacg agatcaagaa cccgctggtc gggatcgcca acggattgga
      121 cctggtgatg cggcgggtgg gggccgaccc ggggctctcg ccgatcgcgg acgagatgtc
      181 gcggcaactg gagcggatcc agggtgcgat ccgggacctg ctggcgtacg cgcggccgtc
      241 ggacccgact cgcgcgccca cggaagtgaa ccgggtggcg aagcgggcgg ccacgctggt
      301 ggggccggcg gcggagcggc ggggtgtgca cgtgcggacg gagctgaacc cggcggtgtc
      361 ggagatcccg gcggacgagg agctgatccg tcaggcgctg gtgaacctgc tgatgaacgc
      421 ggtgcaggcg agcccgccga ccaaggaggt gggggtgagg acggtgacgg cgccggaggg
      481 cgcggcgatc gaggtgtggg atcgaggccg ggggatcacg ccccgggacc tggagcacat
      541 cttcaagccg ttctacacga cccggcacag cgggacgggc ctgggcctga gcatcacgcg
      601 ggagatcgtg gaacggcacg gaggccgcct ggaagtcgag agcgaggtcg gaaagggttc
      661 gaccttccgc atcatcctgc cccgtgaggt cccggccgcg cacggggccg gcggcggctc
      721 gaagggggag gtaggtagcc aatgagcgag gaacgcgtcc tcgtctgcga cgacgagatg
      781 ctcatccgca tgtggctcga ggagcacctg cgggaagcgg gctacgcggt ggagggcacg
      841 ggggacgggg ccgagctcct cgcggcgatg gaacgggagc cggcggacct ggtgctgctc
      901 gatctgcggc tgccggacgg gtcgggcatc gacgtgctcg aacggctgaa gcagcgggag
      961 ccgggggtcc cggtgatcat gatcacggcg tacggtgagg tcgagaccgc ggtggcggcc
     1021 gtgcgcgccg gtgcccacca tttcctggaa aagccgatcc agctctcgga gctcctggtg
     1081 ctcattgagc aggcgctgga gacccgggcc ctgcgcggcg agctcgatcg gtaccgggac
     1141 ggctaccgct ggcagttttc gggggtgacg ttggtcggcc ggtcgcacgc gctgcgcaag
     1201 gtggccgagc tgatcaccag gctgggaatg aagggcagcc ccagcaacgt gctgatccgg
     1261 ggtgagagcg ggacgggcaa ggacgtggtg gcgcgggcgc tccacgcccg cggaccccgg
     1321 cgctcccggc cgttcatctc cgtgaactgc acggcgctac cggaatcgct cgtggaaagc
     1381 gagctgttcg gacacgaggc gggcgcctac acggatgccc gggagaccaa gcgggggctg
     1441 ctggagctcg ccaacggggg cacgatcttc ctcgacgaga tcggggacat gccgaaggcc
     1501 gcccagtcca agctgctcgg cttcctcgag caccgcagct tccggcgggt gggcggcgtg
     1561 cgcgacgtgg aggtggatgt ccacgtgatc gccgccacga atcgcgatct ggaggcggcc
     1621 gtggcggggg gctccttccg ggaggacctc ttctaccgcc tcaacgtggt gccgatctgg
     1681 cttcctcccc tccgcgagcg cccggaggac gtggcgcccc tggtgcttca cctcgtggag
     1741 acgctctgtg gcgacctgcg ccagcccgtc cgggaggtcg agccgggggc gatgcgtgct
     1801 ctggagcgcc acgcctggcc cgggaacgtc cggcagctcc gcaacatcct ggagcgcgtg
     1861 ctcctgctgc acgacgacgg tccgatccat gaggctgatc tcccggcgga gatccggggt
     1921 gggcgggaag aagcggacgg ccgcctggtc gtgctgccgc cttccggaat cagcctggac
     1981 gagctggagc gcgagctcat tgcccaggcg ctggagcgga cggacggcaa caagaccgga
     2041 gccgcccgcc tcctcggggt ctcccgcgac acgctccgat accggctgga gaagtacggg
     2101 atggacgggc cggaataggg ttcgaatcag tcccgtctga gcagcatgcg ggtctcgcgg
     2161 tcgaccgtgg acgggtaggt gtagccgagg tcccgggcga tggcgcgggc ggcgtgggtg
     2221 aagagctcga ccatggcctc gagggcctcc gcgtggcccc ggagggtgtc gcccgcgtag
     2281 gtcgcgtcga gggccgtgcg ctcgtcgggg gcgaggagcc accggagtcc gctgccccgc
     2341 acgccgacgc tctgctccca gccgcgcccg atctggacgt accactcaag catcggcacg
     2401 aggcagcggt agcggaggac ggtctcgagg ctgtagcggg ccgggagcag ctcgccccgg
     2461 ccgatgtact tggccacgta cagcgactcc caccagaact cctcgacgac ggcccggtac
     2521 tcagcgtcac tcgggggccg gggcacgtag gccagcacgg acggcggggg gagccggtcg
     2581 gcgatgcggt cacggtcgag gagcacgcgg tacccggcgg cgagccagtc cggc
//
LOCUS       DASQQQ010000158        17926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_227136, whole genome shotgun sequence.
ACCESSION   DASQQQ010000158 DASQQQ010000000
VERSION     DASQQQ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 17926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17926
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227136"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="VE646_06410"
     CDS             complement(<1..1152)
                     /locus_tag="VE646_06410"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZD79655.1"
                     /translation="MSATMEATSPTPVRVQQSLIPELERFGAVDVSACFNCGNCTAVC
                     PLSTQEGAFPRRLIRYAQVGMRDRLLASPEIWSCYACGECTQTCPRDADPAGFMAAAR
                     RFVTASHDRTGLTRRLATSVPFAAAFITVLVALFGAFMYTAHRPVAGQEIALFEYLPA
                     TFVHNLGIVVLGIFAIAAIGGLVSCCRHLSRVRPMPEEHHRSARRAGEAVWYAVGRES
                     IAQERMRQECADDPDVEPRAWYLRRWFIHASTMWGFLGLLMATILDYLLDLTGVKATG
                     GHVPLWYPVRVLGTVAGLLLVYGTSVSIVRRFHRRERSMAHSSISDWWFLWLLWVAGV
                     SGFALEMALYLPHAPIWGYPMFLFHVAVSMTLVVLAPFGKFAHAIYRPVA"
     gene            complement(1149..1442)
                     /locus_tag="VE646_06415"
     CDS             complement(1149..1442)
                     /locus_tag="VE646_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79656.1"
                     /translation="MSDGNKPGTIGAYKKLHGQASAELLERVRVQNHVKAAVRRSLVD
                     GPKTPPEVAETAELSAREALWTLIALRKYGVVEEVGQDGDYVRYALSGKDPRA"
     gene            complement(1439..3325)
                     /locus_tag="VE646_06420"
     CDS             complement(1439..3325)
                     /locus_tag="VE646_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HZD79657.1"
                     /translation="MAEWSDRPERSQQPEEPERRRIGVYVCHCGGNISDVVDVEAVAD
                     QAAQDEDVVVARHLMFMCSDEGQSTIERDIEDLGLDRVVVAACTPSLHETTFRRTVAR
                     AGLNPYLYQHANIREQVSWAHVHDHAAATEKAARLIRAAIAKAASLRELDEVRVDAVH
                     RALVVGGGVAGLRAALDLARWGMEVVLVERSGGLGGNVLRLGRTYPSEEPGRALVEGL
                     VAAVRSEPSIDVRLWCEVKDVSGYVGDFRVGVHSVDPSGNGHPCEGEVEVGAIVLATG
                     FRDYEPSEGEYGFGHPRVVTLPAYQQRVYAESTDGDLAAALGIPGPVRSVAFLHCVGS
                     RQVEGIDGTDGKPLNTYCSRVCCTASVRAAAETRRRFPEVRVVDVFRDIRTYARDQEE
                     AYREAGEAGVLFVRLPDGERARVETSGNGDGWPLLVRARDSLTFGEELELPVDVVVLA
                     TGMEPGGAEGLVEATKAPTGADGFLLEVHPKLRPVESAIPGILLAGTARGPMDITEST
                     TSASAAAAKVATLLARDQVEMEPFVARVDVGRCTGNGACVAACPYSGALGLQEVELED
                     GVVVTRAYVNPVACKGCGACVAVCPSGAIDVQGWEIGQYVAMVEAITAEEPEVAVGEK
                     AGAR"
     gene            complement(3318..4652)
                     /locus_tag="VE646_06425"
     CDS             complement(3318..4652)
                     /locus_tag="VE646_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HZD79658.1"
                     /translation="MSDRGGREIETDVLVVGAGIGGLQASLDLADQGHRVLLVEREAS
                     IGGRMIALSKVFPTMDCASCITTPKMSSTAHHELITTLTYTDVESISQADGGFAARIV
                     RRPRYVDEDACIGCRLCEYACPVDVPHEFDGGMGARRAVYIPFGTAIPQKAVVDMPNC
                     ILCGKCEKACPTDAIDFLQEPESIQVRARAVVLSTGYKPTPTDAKREYVPAGRTGNVI
                     SGLDMERLLAPNGPYGRVLRPSDGKIPDSIAYVQCAGSRDETVGVPYCSRVCCMYAIK
                     QAMLLSGAIPLADITIYYMDIRTFGKGYEEFYQTARSMGINFVKAKVAKISQRSDGDL
                     DVRIEHQEEGGRVEDVRHDLVVLSVGLEPGADVRRFAPVGADRFGFIRSADPALDATR
                     TDVPGLFAAGTALAPKDIVDTVAEASAVAMQVAAYLRLRSDRPAELAEVAHG"
     gene            complement(4649..5089)
                     /locus_tag="VE646_06430"
     CDS             complement(4649..5089)
                     /locus_tag="VE646_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HZD79659.1"
                     /translation="MTPPTKVLVLASLLCAYPGIDATGQAHLDYPANTYVLPTPDPVM
                     FPEWFYLHAFERGIDGIMIASCGTDSPYEGSYEQLAARINRVYAQMKERGLDIRRLKL
                     TAICSVCTKAFVKEVGDLAATVEGLGPAEEELARSSVAAGSEAS"
     gene            complement(5097..5363)
                     /locus_tag="VE646_06435"
     CDS             complement(5097..5363)
                     /locus_tag="VE646_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HZD79660.1"
                     /translation="MTIALSESDLANLTADKVVDARGSACPGPLLEAKKGIGGVAMNA
                     VLEIWSSDPNTKTDIEAWAGKVGHEFLGSLAADGYDRVFLRRMK"
     gene            complement(5427..5795)
                     /locus_tag="VE646_06440"
     CDS             complement(5427..5795)
                     /locus_tag="VE646_06440"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HZD79661.1"
                     /translation="MADQEKLVIMATHGPEDAELATIPFVMGCAALASDVDAVIGFQA
                     EGVRLVHEGVADKVAAPEFPPLAKLLDDYRELGGKLLVCSPCIKSREIPAEGLVEGAE
                     VVAAARFVAEITSATNSLVY"
     gene            5968..6639
                     /locus_tag="VE646_06445"
     CDS             5968..6639
                     /locus_tag="VE646_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79662.1"
                     /translation="MLPEPTQDRSIEPITGWRIWNLSLPRGREPLLHPAGAGVDAWPR
                     RKPVRARCGTPPLLWFTRRPHQAPNPTCTCGIYASRSLDTFERPRPAYPPPPVIGTVS
                     LWGRVIEHELGWRAQMAYPAQLRLVCSMCAWFEPGPGEPLVVHKFGGRLYTLCEIHRG
                     GIQLPDGRRSKLTPLGPDALQARVLDAYAVEVVPLERVELLFHLPPTPEPPPYMPSIR
                     VVPAR"
     gene            6714..7718
                     /locus_tag="VE646_06450"
     CDS             6714..7718
                     /locus_tag="VE646_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005517358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HZD79663.1"
                     /translation="MPAITVEDLTRLQRVPPVAGTARLRPVRRVSLAPHALEGEGFPV
                     RRAFAGADLTDLDPFVHMDQMGEVEYAPGEPKGTPWHPHRGFETVTYMLDGIFEHQDS
                     NGGGGVITNGDTQWMTAGAGILHIEKPPEHLVLSGGLFHGIQLWVNLPRAEKWAPPRY
                     QDLRAGEVVLLATPDAGALVRVIAGEIAGHPGPGQTYTPMTMLHATVSPGARLELPWR
                     PDFNALAYVLAGIGTVGAEQQPIGTGQLAVFGPGDAVRLGAATAQESRSPSLEVLLLG
                     GRPIREPVAWYGPFVMNTKAELLQAFEDYQAGRLGQIPAAHHTPDHIVESPPGKDPGA
                     "
     gene            complement(7757..7981)
                     /locus_tag="VE646_06455"
     CDS             complement(7757..7981)
                     /locus_tag="VE646_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZD79664.1"
                     /translation="MQVHVTLRGTLTDRLPGGKGTLDVADGATVATVSERLGLPGGHC
                     VFVLNGATVKLGAELREGDRLQAFPPMAGG"
     gene            complement(8013..9356)
                     /locus_tag="VE646_06460"
     CDS             complement(8013..9356)
                     /locus_tag="VE646_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HZD79665.1"
                     /translation="MAVREAPTGPALDHVVVDRYTDLVGPGNEMLGPVRDGGRIEYLT
                     VPGCWGPMITPSIRSGHEVSLPVRVEGAKVGDAVALHVETLEQLSRATTSGTHTGWEG
                     RFISDPFVANICPTCRDAGRHFLYPDTYLDGIGEGAVRCSTCHSEVIPFHMVEGYTMV
                     FDEGMSLGVTVAPERAEELAREAREWVVLQEHATQHPIVALGKADLVGAVTRVRVSAG
                     NLGSIPAIDLPSSHNCGDFGAFLVGAEHEFAVSEEQLELRTDVHMDIDSVREGSIVIV
                     PVKVDGAGIYAGDVHSMIGDGEVAVHTTDVSARVVVRVEVIRGLELDGPILLPPAEDL
                     PFLAKPFTKDEVDRAMALARMHKTKLEGPVMPIQVLGSGPFINAAVDNGVERAARLLR
                     MSVDEVKNRTTVSGAVEIGRLPGFVQINLLAPRERLERLGIWHLVEQMYGAPTGW"
     gene            complement(9484..12711)
                     /locus_tag="VE646_06465"
     CDS             complement(9484..12711)
                     /locus_tag="VE646_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZD79666.1"
                     /translation="MRCPRCGSEGPAGARYCPACGAELKALATEERKLVSVLFVDQVG
                     STARADGADPEDVRDENRRFFDEVRGRIERHGGVVEKYIGDAVMAVFGAPIGRSDDAE
                     RAVRAALAVAEGVAELMAEGLDLQVRAAVCTGEAIVALEPPPGDALATGDVVHVAARL
                     QAAAPPGGTIVDAETHRLTRHAFGFQELAPIRAKGKAAPVPVWLVGEPVGAPALRPVS
                     TTPLVGREREQGLIRMVWQGAVSSRRPHLVTILGPSGIGKSRLAREVSAEVEAGDGQA
                     LWGRSLPYEEQTPHHALGQIVRHLAQIYENDPAEVARTKLAILVGRLFVEPEAPDTTR
                     YLSLLLGLGLDEPTSDPIHLRYASRLLIEHLAESRPVMIVFEDLHWADDALLELIDYL
                     VAHVHDASVVFLALARPEFVERRPGWGAGLIGQTTLPLEPLTTDEAGRVAASLLGGAR
                     PEVIERVVAAAGGNPLFLEELVASIGEAAPGELPSTIRAAVGARIDALPPDVRTLLLY
                     ASVIGRSFWQGVLERVTEAADVGSALEELEARGLVLRRPRSQVEGDAEFSIKHDLIRD
                     VAYETLPRATRRRLHAATAEAIETSVQEPRELAWILAHHWREAGEPERAIAYFLDAAD
                     RARGALAVEETYDYYARALELAGDDDHRRQIRLRRGLALVELEDYARAERELGELLGE
                     LEGQDQLEALLARSHATLWTERTDETLDQARRLVGLVGNRGVVELQGPALAILAAAYA
                     MRGEDGDLEEAISSGDRALEVWVPGTRRRELAEHYHLHADHAYWTGDYGRALELTRLA
                     AETGGGEPHSIEFVLRGAGMEGLVLAGLGRYEEALQTNEAAIGIAREMGRSTNVVVNY
                     STSVLRDIFDLEEARRRSEEVADRLGPSEFNMPWMNARADVIAAAILQGDLGTAERLW
                     PSAWEDALGSVAWEHWLVSGRLAAARAELELEMGRPDEAVAWARRALTLARRTRRRKY
                     EAVTLETLGRALTAQGLADRAVSELRRAVSAADAVGSPLARWRARAALAGALARSSER
                     DGESEVVWGEAAGIVRRVLGDLAPKRAARYRSDRRVAEVLAAAPS"
     gene            12811..14262
                     /locus_tag="VE646_06470"
     CDS             12811..14262
                     /locus_tag="VE646_06470"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HZD79667.1"
                     /translation="MDRAPAILNLLDEDELRRVHDSALRVLAEVGARIMSGEGRRLLL
                     EHGGTLLDEDVVRVPATLVERALADAPHRFTMFDRNGAPALHLGEGTVSVGAGVTNLN
                     YLDPETDRVEPFSLEATARSTLLADALPNIGFVATPGVTRATEDLPQHIVNQREFVEM
                     VANTTKPLMVLIAGGPELSDIYHMAELAAGGPDALRRRPFVIPYLNSVSPLLFNPETV
                     DKLFLAADRGLPVCCQAAPQVGATGPATIAGTLVVAAAETLLGLVLTQLRHPGLPFVS
                     GTVPFLMDMRNGAVTAGGPDGLRFMVAMGQLCRRWGLPLVGMSFGGDSKVMDEQAVLE
                     ATYYGLGATLAGVDLIFDAGCVEGGLLFSPELLVIADQAVEMARRATERLDLSEEAIA
                     FETIARVGPGGLYLGEEHTLSHFRELWLPRILSWEPRPQWEEGGATTLRQRARERVLA
                     TWGEHTVDPLPEDVLAGMREVIERRRATPPPPE"
     gene            14490..15395
                     /locus_tag="VE646_06475"
     CDS             14490..15395
                     /locus_tag="VE646_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZD79668.1"
                     /translation="MQLIKRRLVGKEGSARVKELRAILADLPGYKNGPYADIRAWVAG
                     QIEQTRVRSRVVHRDSIAVRREGQAQIALVGPPNVGKSSLLQALSDIQIRTGDYPFTT
                     LRPVAAVAVIHGIPVQLVEIPGLIEGATKDRGGGRALLGVLRGADAIVFCHDATAPLQ
                     PLRQVLAEVRAAGIDRPALLTATKLDEAGTSALERLRATVPQLPVMGVSILDDASLDA
                     FREAVWGLTGLVRVFTRRPGSPQADPLAVRAGATVEDVAERLHGELAGSCRGARVWGP
                     SGRFPGQRVGREHAVLEGDVIEVLA"
     gene            complement(15413..16270)
                     /locus_tag="VE646_06480"
     CDS             complement(15413..16270)
                     /locus_tag="VE646_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZD79669.1"
                     /translation="MDIAIGIGNTLLDMEGPTLLEWARRAEARGFSSLATIGRVAWPG
                     FDELAALAAAAAVTSRIGLLTDVLLAPTYDAARLAKLTASVDALSAGRLTLGLGVGAR
                     PDDFPAVGRDFHARGRIFDEQLGRLHESWAGQGSALGPTPFGPATLRGRIPVLFGGDP
                     VRAARRAARWEGGYTVGGAPAEAAEGMLAAFKGAFRQVGGTRTPRTVCLAYFGLDERD
                     EESRRQLLAYYGYVGDRAQAIATGAARTAEQVRDRVEAYRRLEIDELVFVPSVPEVDQ
                     VDLLADLVL"
     gene            16389..17837
                     /locus_tag="VE646_06485"
     CDS             16389..17837
                     /locus_tag="VE646_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZD79670.1"
                     /translation="MATRWAATCGTRPGGARLVVAAAAVMVLTACSSNGPSSPAPSSG
                     AGSAPSTVPAHVSPSPLPSGPAAGAAWTTYGGDAARSGLDRSSPPIGRVHRVWTSPAL
                     DGAIYAQPLLFRERVFVATESDSVYALDAASGQVAWRRHLGEAVPRSALPCGNIDPTG
                     ITGTPVIDPSTGVLYAVTFVQPGRHDLVALDTADGSVRFRRPIDPPGLDPLVEQQRPA
                     LTISDGRVYVAYGGLFGDCGEYHGAVVGSALDGSGSLISYRVPSGRAAGIWAPPGPTV
                     VPGDDLLVATGNSFSTSSFDFGNAVIRLSPSLDVVDWFAPSNWLDLNRGDVDLGSTSP
                     ALLPDGLVFIAGKEGIGSLLRLDRLGHVGGEVFSARVCDRAFGGTAVRPPFVYVSCTG
                     GVVGLRLGSGPSFQVAWRGPSFDAGPPIVSGGAVWTVDLGSGDLVALDAGTGDQRSRQ
                     RLGAVVHFTAPAAGDGHVYVAATDRVVGVAGG"
BASE COUNT         2463 a         6773 c         6168 g         2522 t
ORIGIN      
        1 cgccaccggc cggtagatcg cgtgcgcgaa tttcccgaac ggcgccagca ccacgagggt
       61 catggagacg gccacgtgga acaggaacat cgggtacccc cagatggggg catggggcag
      121 gtacagggcc atctccagcg cgaagcccga gacgcccgcc acccacagga gccacaggaa
      181 ccaccagtcg gagatcgagg agtgggccat cgagcgctcg cgcctgtgga accggcgcac
      241 gatcgacacc gacgtcccgt acacgagcag cagcccggcc acggtgccca gcacccggac
      301 cgggtaccag agcggcacat gcccgccggt agccttgacc ccggtcaggt ccagcaggta
      361 gtcgaggatc gtggccatga gcagaccgag gaacccccac atcgtggagg cgtggatgaa
      421 ccaccgccgc aggtaccagg cccggggttc cacgtcgggg tcgtcggcgc actcctggcg
      481 catccgttcc tgggcgatcg actcccgccc caccgcgtac cacacggcct ctccggcccg
      541 ccgagccgac cgatgatgct cttcgggcat cggtcgcact cgcgacagat gtcgacagca
      601 gctcaccagc ccgccgatcg cggcgatggc gaagatcccg agcacgacga tccccaggtt
      661 gtggacgaac gtggccggga ggtactcgaa cagcgcgatc tcctgcccgg ccaccggacg
      721 gtgcgccgtg tacatgaacg cgccgaacag ggcgaccagc accgtgatga acgcggccgc
      781 gaacggcacc gaggtggcca ggcgccgggt caggccggtg cggtcgtggc tcgccgtgac
      841 gaagcggcgg gccgccgcca tgaagccggc cgggtccgca tcccgcggac aggtctgcgt
      901 gcactcgccg cacgcgtagc aggaccagat ctcggggctg gccagcagcc ggtcgcgcat
      961 cccgacctgc gcgtagcgga tcagccgccg cgggaaggcc ccttcctgcg tggagagggg
     1021 gcacaccgcc gtgcagttgc cgcagttgaa gcaggcggac acgtccacgg ctccgaaccg
     1081 ttccagctcc gggatcaggc tctgctggac ccggaccggc gttgggctgg tggcttccat
     1141 cgtggcgctc atgcccgcgg atccttcccg ctcagggcgt accggacata gtccccgtcc
     1201 tggccgacct cctcgaccac gccgtacttg cgcagggcga tcagcgtcca cagggcctcg
     1261 cgcgccgaga gctcggcggt ctccgcgacc tccggggggg tcttcggccc gtcgaccagc
     1321 gatcgtcgga ccgcggcctt gacgtggttc tggacgcgga cccgctcgag cagctcggcc
     1381 gaggcctgcc cgtggagctt cttgtaggcg ccgatcgtcc ccggcttgtt cccgtcgctc
     1441 atcgggctcc cgccttctcg cccaccgcca cctccggctc ctcggccgtg atggcctcga
     1501 ccatggcgac gtactggccg atctcccatc cctggacgtc gatcgcgccg ctcgggcaga
     1561 cggccacaca tgcgccgcag cccttgcagg ccaccgggtt cacgtaggcc cgcgtcacca
     1621 ccacgccgtc ctcgagctcc acctcctgca acccgagcgc tccggagtac gggcaggcgg
     1681 ccacgcacgc cccgttcccc gtgcagcggc cgacatccac ccgggccacg aacggctcca
     1741 tctccacctg gtcgcgggcc agcagggtgg cgaccttggc cgcggcggcc gatgccgagg
     1801 tcgtgctctc ggtgatgtcc atcgggcccc gggccgtccc ggccagcagg atgccgggga
     1861 tcgcgctctc caccggacgg agcttggggt ggacctcgag caggaaaccg tccgcaccgg
     1921 tgggggcctt cgtggcctcc accaggcctt cggcgccacc gggctccatg cccgtggcca
     1981 ggaccacgac gtccaccggc agctccagct cctcgccgaa ggtgagcgag tctcgggctc
     2041 gcaccagcag cggccagccg tcgccgttcc cgctcgtctc caccctggcc cgctccccgt
     2101 cggggaggcg cacgaacagg accccggcct caccggcctc ccggtaggcc tcctcctggt
     2161 cgcgggcgta ggtgcggatg tcccggaaca cgtccaccac cctgacctcg gggaagcgcc
     2221 gccgggtctc cgcggccgcc cggacgctgg ccgtgcagca gacccgggag cagtacgtgt
     2281 tcaggggctt cccgtccgtc ccgtcgatgc cctccacctg gcggctgccc acgcagtgca
     2341 ggaacgccac cgagcggacc ggccccggga tccccagggc agcggccaga tcgccgtcgg
     2401 tgctctccgc gtacacccgc tgctggtacg ccggaagcgt gaccacccga gggtgaccga
     2461 acccgtattc gccctcggac ggctcgtagt cccggaagcc ggtggccagc acgatcgcgc
     2521 ccacctcgac ctcgccttcg cacgggtgcc cgttgccgct cgggtcgacc gagtggacac
     2581 cgacccggaa gtcgcccacg tagccggaga cgtccttcac ctcacaccac aggcggacgt
     2641 cgatcgaggg ctcggaccgc acggcggcca ccaggccttc cacgagcgca cgaccgggct
     2701 cctcggacgg gtacgtgcgt cccaggcgga ggacgttgcc gccaagcccc ccgctgcgct
     2761 cgaccagcac cacctccatc ccccaacggg ccagatctag cgcggcgcgg agcccggcca
     2821 ccccgccgcc cacgaccagg gcgcggtgga ccgcgtccac ccgtacctcg tcgagctctc
     2881 gcagcgacgc ggccttcgcg atcgccgccc ggatcagacg ggcggccttc tcggtggcgg
     2941 ccgcgtgatc gtggacgtgc gcccagctga cctgctcgcg gatgttcgcg tgctggtaca
     3001 ggtaggggtt cagcccggcc cgggccacgg tgcgtcggaa cgtggtctcg tgcaggctgg
     3061 gggtgcaggc cgccaccacc acccgatcca ggccgaggtc ctcgatgtcc cgctcgatcg
     3121 tgctctgtcc ctcgtccgag cacatgaaca tcagatgccg ggcgacgacg acgtcctcgt
     3181 cctgcgcggc ctgatcggcg acggcctcga cgtcgacgac gtcgctgatg ttcccgccgc
     3241 agtggcacac gtagacgccg atccggcggc gctccggttc ctccggctgc tgcgaccgct
     3301 ccggccgatc cgaccactca gccatgggcc acctccgcca gctccgcggg tcgatcgctc
     3361 ctcaggcgca ggtaggccgc cacctgcatg gccaccgcgc tcgcctcagc gaccgtgtcg
     3421 acgatgtcct tgggcgcgag cgcggtaccc gcagcgaaga gcccggggac atcggtccgg
     3481 gtggcgtcga gcgcggggtc cgccgaccgg atgaagccga agcgatcggc ccccacgggc
     3541 gcgaaccgcc gcacgtcggc ccccggctcc agccccaccg agagcacgac caggtcgtgc
     3601 cggacgtcct cgacccggcc cccctcctcc tgatgctcga tccgaacgtc caggtcgccg
     3661 tcggaccgct gggagatctt ggcgaccttc gccttcacga agttgatccc catggaccgg
     3721 gcggtctgat aaaactcctc gtaccccttg ccgaaggtcc ggatgtccat gtagtagatc
     3781 gtgatgtcgg ccagagggat cgcgcccgag agcagcatgg cctgcttgat cgcgtacata
     3841 cagcacacgc gggagcagta cggcacgccg accgtctcgt cccgggagcc cgcgcactgc
     3901 acgtaggcga tgctgtccgg gatcttcccg tcggaggggc gcagcacccg tccgtagggg
     3961 ccgttcggcg ccagcagccg ctccatgtcc aggccgctga tcacgttccc ggtcctgccg
     4021 gccggcacgt actcgcgctt ggcgtcggtc ggggtgggct tgtagccggt gctcaggacc
     4081 accgcacgcg cgcggacctg gatgctctcc ggctcttgga ggaagtcgat ggcgtcggtg
     4141 ggacaggcct tctcgcactt gccgcagagg atgcagttgg gcatgtccac cacggccttc
     4201 tgcgggatcg ccgtcccgaa ggggatgtag acggcccgcc gggcccccat ccctccgtcg
     4261 aactcgtgcg ggacgtccac ggggcaggcg tactcgcaca gccggcaccc gatgcaggcg
     4321 tcctcgtcca cgtatcgcgg cctccggacg atgcgcgccg cgaagccgcc gtccgcctgg
     4381 ctgatggact cgacgtccgt gtaggtgagc gtggtgatga gctcgtggtg ggcggtcgag
     4441 gacatcttgg gcgtggtgat gcagctggcg cagtccatcg tggggaagac cttcgacagg
     4501 gcgatcatcc gtcccccgat gctcgcctcc cgctccacca ggagcacgcg gtggccctgg
     4561 tcggccaggt cgagggaagc ctgcaggccg ccgatcccgg cccccacgac caggacgtcc
     4621 gtctcgatct cccggccccc tcggtcgctc acgacgcctc gctccccgcg gcgaccgagc
     4681 ttcgggcgag ctcctcctcg gccggcccga gcccctcgac cgtggcggcc agatcgccga
     4741 cctccttcac gaacgccttc gtgcacaccg agcagatcgc ggtgagcttc agccgacgga
     4801 tatccaggcc ccgctccttc atctgcgcgt agacgcggtt gatccgggcg gctagctgct
     4861 cgtaggagcc ttcgtacgga ctgtccgtcc cgcaggaggc gatcatgatt ccgtcgatcc
     4921 cgcgctcgaa cgcgtgcagg tagaaccact ccgggaacat caccggatcc ggcgtgggca
     4981 gcacgtacgt gttggccggg taatccaggt gggcctggcc ggtggcgtcg atccccggat
     5041 aggcgcacag gagcgacgcg agcacgagga ccttcgtggg aggcgtcacc gccgcctcac
     5101 ttcatccggc gcaggaacac gcggtcgtag ccgtcggcgg ccagggaccc gaggaactcg
     5161 tggcccacct tgccggccca ggcctcgatg tcggtcttcg tgttcggatc gctggaccag
     5221 atctccagca cggcgttcat cgccacgccg ccgatgccct tcttggcctc gagcaggggt
     5281 ccggggcacg ccgagccccg ggcgtcgacg accttgtcgg ccgtcaggtt cgcgagatcg
     5341 ctctcgctga gcgcgatggt catgttcgaa ccctccttcg cggccgcgcg ggcgaccggc
     5401 ccgcgaggcc gggttccgcg gtgcgatcag tagacgaggc tgttcgtcgc cgaggtgatc
     5461 tcggccacga agcgggcggc cgccaccacc tcggccccct ccacgagccc ctccgccggg
     5521 atctcgcggc tcttgatgca ggggctgcag acgagcagct tcccgcccag ctcgcggtag
     5581 tcatcgagca gcttcgccag cggtgggaac tcgggggccg ccaccttgtc ggccactcct
     5641 tcgtgcacga ggcgaacgcc ctcggcctgg aacccgatga cggcgtcgac gtccgaggcg
     5701 agcgccgcgc accccatcac gaaggggatc gtcgcgagct ccgcgtcctc ggggccgtgg
     5761 gtcgccatga tgacgagctt ctcctggtct gccatggccg gcacctcctg tgcgggctgt
     5821 atgtctgcgg ccaacatagg tctgcgacac agggcgggag ggggccggag ggtcccgttc
     5881 ccggccgccg atggtcccgc tccccggcca tcgctcgggg cggtctgcct tgccgtgcgc
     5941 cggcggtcag cccaccatgg aagggtgatg ctccccgagc ccacccagga tcgttccatc
     6001 gaacccatca ccggatggcg catctggaac ctgtccctgc cccggggacg ggagccgctc
     6061 ctgcacccgg ccggtgcggg cgtggacgcc tggcccaggc gcaagccggt ccgggcccgt
     6121 tgcgggacgc cgccactgct gtggttcacg cggcggccgc accaggcccc gaaccccacc
     6181 tgcacctgcg ggatctacgc cagccggtcg ctcgacacct tcgagcggcc tcgccccgcc
     6241 taccccccac ctccggtgat cggcacggtc tccctgtggg gacgggtgat cgagcacgag
     6301 ctcgggtggc gagcccagat ggcatacccg gcccagctcc ggctggtgtg ctcgatgtgc
     6361 gcctggttcg agcccggtcc gggggagccg ctcgtcgtcc acaagttcgg gggccggctg
     6421 tacacgttgt gcgagatcca tcggggcggg atccagctcc ccgacgggcg gcgctccaag
     6481 ctcacccccc tgggtccgga cgcgctgcag gccagggtgc tggacgccta tgccgtggag
     6541 gtggtgcccc tcgagcgggt ggaactgctg ttccacctcc cgcccacgcc cgagccaccg
     6601 ccgtacatgc cgtcgatccg ggtggtcccg gcccgctgag cagaggcccc cgagggagcc
     6661 catcggaata ccgaggccgc gggccgcgtt gcccggcagg aaggagccaa ccgatgccag
     6721 cgatcaccgt cgaggacctg acccggttgc agcgcgtgcc ccccgtcgcc gggacggcgc
     6781 ggctccgacc ggtgcgccgg gtctcgctgg ctccccacgc gctcgagggg gagggcttcc
     6841 ccgtgcgccg ggccttcgcc ggcgccgatc tcaccgacct cgaccccttc gtccacatgg
     6901 accagatggg cgaggtcgaa tacgcgccgg gcgagcccaa gggcacgccc tggcaccccc
     6961 accgcgggtt cgagaccgtg acctacatgc tggacgggat cttcgagcac caggactcga
     7021 acggcggcgg gggcgtgatc acgaacggcg acacgcagtg gatgacggcc ggggccggga
     7081 tcctgcacat cgagaagccc cccgagcacc tggtgctctc cggtggcctg ttccacggga
     7141 tccagctttg ggtcaacctg ccccgggccg agaagtgggc tccgccccgg taccaggacc
     7201 tgcgggccgg cgaggtcgtt ctgttggcga ccccggatgc cggggcgttg gtgcgggtca
     7261 tcgccgggga gatcgccggc caccccggcc ccgggcagac gtacacgccc atgaccatgc
     7321 tccatgcgac ggtgtcgccc ggggcgcgcc tggagctccc ctggcgcccc gacttcaacg
     7381 cgctcgccta tgtgctggcc ggcatcggca cggtcggcgc ggagcagcag ccgatcggta
     7441 ccggacagct ggccgtgttc ggccccggcg acgccgtccg gctgggggcg gccacggccc
     7501 aggagtcacg ttcgccgagc ctggaggtcc tgctgctggg gggccgcccc atccgcgagc
     7561 cggtggcctg gtacgggcca ttcgtcatga acacgaaggc cgagctgctc caggcgttcg
     7621 aggactacca agcggggcgc ctgggccaga tccccgcggc ccaccacacg cccgaccaca
     7681 tcgtcgagtc cccgcccgga aaggacccgg gcgcctgagg ggcggcctcg ccgttcggcc
     7741 cggggctggc gcggcgtcag ccgccggcca tcggcgggaa ggcctgcagg cggtcaccct
     7801 cacgcagctc ggccccgagc ttcaccgtgg cgccgttcag gacgaacacg cagtgcccgc
     7861 ccggcaggcc gagccgctcc gacaccgtgg cgacggtcgc tccgtcggcc acgtccagcg
     7921 tccccttccc gccgggcagg cgatcggtca acgtgccccg aagggtcacg tggacctgca
     7981 cggtgcgcct ccgccggccg gcagccgtgc cgtcaccagc cggtcggcgc gccgtacatc
     8041 tgctccacga ggtgccagat cccgagccgc tccagccgct cccgcggcgc cagcaggttg
     8101 atctggacga accccggcag acggccgatc tccaccgccc cgctcaccgt ggtccggttc
     8161 ttcacctcgt ccacgctcat ccggagcagc ctcgcggctc gctccacgcc gttgtccacc
     8221 gccgcgttga tgaacgggcc cgagcccagg acctggatcg gcatcaccgg gccctcgagc
     8281 ttggtcttgt gcatcctcgc gagggccatg gcccggtcga cctcgtcctt cgtgaagggc
     8341 ttggccagga acggcaggtc ctcggccggc gggagcagga tcggcccgtc cagctcgagg
     8401 cccctgatca cctcgacccg gacgaccacc cgggcggaga cgtcggtggt gtgcacggcg
     8461 acctcgccat cgccgatcat ggagtgcacg tccccggcgt agatcccggc cccgtccacc
     8521 ttcaccggga cgatcacgat ggagccctcc cgcaccgagt cgatgtccat gtgcacgtcg
     8581 gtgcggagct ccagctgctc ctcgctgacg gcgaactcgt gctcggcccc caccaggaag
     8641 gccccgaagt cgccgcagtt gtgtgaggag ggcaggtcga tcgcggggat cgagcccagg
     8701 ttcccggccg agacccggac cctggtcacc gcgcccacca ggtccgcctt gccgagggcg
     8761 acgatcggat gctgcgtggc gtgctcctgc agcaccaccc actcacgggc ctcgcgggcc
     8821 agctcctcgg cccgctcggg cgccacggtc acccccaggg acataccctc gtcgaagacc
     8881 atcgtgtagc cctcgaccat gtggaagggg atcacctccg agtgacaggt cgagcaacgg
     8941 accgccccct cgccgatccc gtccaggtag gtgtccgggt acaggaagtg acggcccgcg
     9001 tctcggcacg tggggcagat gttggccacg aaggggtccg agatgaaccg cccctcccag
     9061 ccggtgtgcg ttccggacgt ggtagcgcgc gagagctgct ccagggtctc gacgtggagc
     9121 gcgacggcgt cgcccacctt ggcgccctcc acacgcaccg gcagcgagac ctcgtggccg
     9181 ctgcggatcg agggcgtgat catcggtccc cagcagccgg ggaccgtcag gtactcgatc
     9241 cggccgccgt cgcggaccgg cccgagcatc tcgttgccgg gcccgaccag atcggtatac
     9301 cgatccacca ccacgtggtc cagcgccgga ccggtgggag cctcacgaac ggccatcgcc
     9361 tggtcacctc ctcgcaccgc cggggccgtc gagggccgac ccggcggacg gcgatcacgg
     9421 gttgcagccc catcgtccgc ccgacgaccg gacctggtca aaccgctctc ggcgagcgcg
     9481 aggtcacgag ggtgcggcgg ccaggacctc ggccacccga cggtccgatc ggtaccgggc
     9541 ggcccgtttc ggagccagat cgcccagcac ccggcggacg atccccgccg cctcacccca
     9601 caccacttcc gactccccat cgcgctccga ggagcgagcc agcgcaccgg ccagggccgc
     9661 gcgggctcgc caccgggcca ggggcgagcc cacggcgtcg gcggcagaca cggctcgccg
     9721 cagctcggag acggcccgat cggccagccc ttgggccgtc agggcccgac ccagcgtctc
     9781 caacgtcacc gcctcgtact tgcggcgccg ggtcctacgg gccagcgtca gggcccgtcg
     9841 ggcccaggcc accgcctcat ccgggcgccc catctcgagc tcgagctcgg cgcgcgctgc
     9901 ggccagacgc ccgctcacca accagtgctc ccatgccacg ctccccagcg cgtcttccca
     9961 cgccgacggc cacaggcgct cggcggtccc caggtcgccc tggaggatgg cagccgcgat
    10021 cacgtccgcc cgggcgttca tccacggcat gttgaactcc gacggcccca gccggtccgc
    10081 cacctcctca ctccggcgcc gggcttcctc gagatcgaag atgtcccgga ggacggaggt
    10141 cgagtagttg accaccacgt tggtgctgcg acccatctcc ctggcgatcc cgatggcggc
    10201 ctcgttggtc tggagggctt cctcgtaccg ccccagcccg gccaggacga gcccctccat
    10261 ccccgcgccc cgcaggacga actcgatgct gtggggctcc cccccgccgg tctcggcggc
    10321 cagccgggtg agctccaacg cgcgcccgta gtcgccggtc cagtacgcgt ggtccgcgtg
    10381 gaggtggtag tgctcggcga gctcgcgccg ccgggtcccc ggaacccaca cctccagggc
    10441 gcggtcgccc gaggagatcg cctcctccag gtcaccgtcc tcaccccgca tggcgtaggc
    10501 ggcggccaag atcgcgagcg cgggcccctg gagctcgacc acgccgcggt tcccgaccag
    10561 accgaccagc cgtcgggcct ggtccagggt ctcgtcggtg cgttccgtcc agagcgtcgc
    10621 gtggctgcgg gccaacagcg cctcgagctg gtcctgcccc tccagctcgc cgagcagctc
    10681 ccccagctcg cgctccgccc gcgcatagtc ctccagctcc accagggcca ggccgcggcg
    10741 caggcggatc tggcgccggt ggtcatcgtc ccccgcgagc tccagcgctc gcgcgtagta
    10801 gtcgtacgtc tcctccacgg ccagcgcgcc ccgcgctcga tccgccgcat ccaggaagta
    10861 ggcgatggcc cgttctggct cgccggcctc ccgccagtgg tgagccagga tccatgccag
    10921 ctcccggggc tcctggaccg aggtctcgat cgcctccgcg gtcgcggcgt ggaggcgccg
    10981 acgggtggcc cggggcagcg tctcgtacgc cacgtcgcgg atcaggtcgt gcttgatgga
    11041 gaactccgca tccccctcga cctggctgcg aggccgtcgc aacaccagcc ccctcgcctc
    11101 caactcctcg agcgccgacc ccacgtctgc cgcctcggtg acccgttcca gcacgccttg
    11161 ccagaacgag cgcccgatca cggaggcata caggagcagc gtccggacgt ccggcggcaa
    11221 ggcgtcgatc ctggccccca cggccgctcg gatggtggag ggcagctcgc cgggtgcggc
    11281 ctccccgatg gaggccacca gctcctccag gaacagggga ttgccccccg ccgccgccac
    11341 cacgcgctcg atcacctcgg gccgtgcccc accaaggagg gaggcggcga cccgaccggc
    11401 ctcgtcggtg gtgagcggct ccagcggcag ggtggtctga ccgatgaggc ccgcgcccca
    11461 ccccggccgt cgctccacga actcggggcg cgcgagggcg aggaagacca ccgacgcgtc
    11521 gtggacgtgc gccaccaggt agtcgatgag ctccaggagc gcgtcgtccg cccagtggag
    11581 gtcctcgaac acgatcatca cgggtcggga ctccgccagg tgctcgatca ggagccggct
    11641 cgcgtaccgg agatggatcg gatcgctggt cggctcgtcc aggcccaagc cgagcagcag
    11701 cgagaggtac cgcgtggtgt cgggcgcctc cggctcgacg aacagccttc ccaccaggat
    11761 cgccagcttg gtccgagcga cctccgccgg gtcgttctcg tagatctgcg ccaggtggcg
    11821 gacgatctgg cccaacgcgt ggtgaggtgt ctgctcctcg tagggcaggc tgcgacccca
    11881 cagggcctgt ccgtcacccg cctccacctc ggcggagacc tcacgagcca accgggactt
    11941 gccgatcccg gagggtccga ggatcgtcac caggtgcggg cgccggctgg agacggcccc
    12001 ctgccacacc atccggatca gcccctgctc gcgttctcgc cccaccaggg gggtggtcga
    12061 gaccggccgg agcgcgggtg cacccaccgg ctcgcccacc agccagacgg gcacgggggc
    12121 cgctttgccc ttggcccgga tgggggccaa ctcctggaac ccgaaggcgt ggcgcgtgag
    12181 gcgatgggtc tccgcatcga cgatcgtccc ccccggcggc gcggccgcct ggagccgggc
    12241 ggccacgtgc accacgtctc cggtcgccag cgcgtccccc ggcgggggct ccagcgcgac
    12301 gatcgcttct ccggtacaca cggccgcccg cacctgcaga tccagcccct cggccataag
    12361 ctcggcgaca ccctccgcga cggccagggc ggcccggacg gcgcgctcgg catcgtcgct
    12421 gcgaccgatg ggcgccccga acacggccat gaccgcatcg ccgatgtact tctcgaccac
    12481 gccgccgtgg cgctcgatgc gaccccgcac ctcgtcgaag aaccggcggt tctcgtcccg
    12541 gacgtcctcc ggatccgctc catctgccct ggcggtggag cccacctggt cgacgaagag
    12601 gaccgagacg agcttgcgct cctcggtggc cagagccttg agctcggcgc cacacgcggg
    12661 gcagtaccga gcacccgccg gcccctcgga cccgcagcgg ggacagcgca tgccgcatcc
    12721 tacgtgatgg caggggggcc cgcgccaccc gagggtctcg ggccgttcgg ccctggcccg
    12781 gcgaccgacc cggccgggac catgccggtc atggatcgcg cccccgccat cctgaacctg
    12841 ctggacgagg acgagctcag gcgggtgcac gacagcgcgc tgcgcgtgct ggccgaggtg
    12901 ggagcccgca tcatgagcgg cgagggccgg cgcctcctgc tcgagcacgg cggcaccctg
    12961 ctcgacgagg acgtggtgcg ggtgccggcc accctggtgg aacgggcgct ggccgacgcc
    13021 ccccaccgct tcaccatgtt cgaccggaac ggggcgcccg ccctgcacct gggcgagggc
    13081 acggtgtccg tgggggccgg cgtgaccaac ctgaactacc tggacccgga gaccgaccgc
    13141 gtggagccct tctcgctcga ggccaccgca cggtcgaccc tgctggccga cgcgctgccg
    13201 aacatcggct tcgtggccac gcccggcgtc acgcgcgcca ccgaggacct accccagcac
    13261 atcgtgaacc agcgcgaatt cgtggagatg gtggcgaaca ccaccaagcc gctcatggtg
    13321 cttatcgccg gtggtcccga gctgtcggac atctaccaca tggccgagct ggcagccggt
    13381 ggcccggatg ccctgcggag gcggccgttc gtgatcccgt acctgaactc ggtctccccg
    13441 ctgctgttca acccggagac cgtggacaag ctcttcctag ccgccgatcg aggcctgccg
    13501 gtgtgctgcc aggccgcccc ccaggtgggc gcgaccgggc cggccacaat cgcggggacc
    13561 ctggtggtcg ccgccgccga gacgctgctg gggctggtgc tgacccagct ccgtcacccc
    13621 ggcctgcctt tcgtgagcgg gaccgtcccg ttcctgatgg acatgcgaaa cggggccgtg
    13681 acggccggcg gcccggacgg cctgcgcttc atggtggcca tgggccagct gtgtcggcga
    13741 tggggcctgc cgctcgtggg gatgagcttc ggcggggact cgaaggtcat ggacgagcag
    13801 gccgttctcg aggctaccta ctacgggctc ggcgcgacgc tggcgggagt cgacctgatc
    13861 ttcgacgcgg gctgcgtgga gggcggcctg ctgttctccc ccgagctgct ggtgatcgcc
    13921 gaccaggccg tggagatggc ccgccgcgcc accgagcgcc tggacctgag cgaggaggcg
    13981 atcgccttcg agacgatcgc gagggtcggg cccggcggcc tgtacctggg cgaggagcac
    14041 acgctcagcc acttccggga gctgtggctc ccccggatcc tgtcctggga gccgcgcccg
    14101 cagtgggagg agggcggggc caccacgctt cgtcagcggg ctcgggagcg tgtgctggcg
    14161 acctgggggg aacacacggt cgatcccctg ccggaggacg tgctcgccgg gatgcgcgag
    14221 gtgatcgaac gacgtcgggc caccccaccg ccgccggagt gagcgaggcg ccactcgtgt
    14281 atcctgcagc gcatggcact gcgcaaggaa gcgtgttggc gcctggaggt cttcaggcag
    14341 tcgcactagg ccgctgcttc ccggttccgc gtcgggggaa gcagcgggga caggcgcatc
    14401 catgacggtc ggcacccgcc ggccgggtgt cgcctcctcc cgctccatcg caggaggtcg
    14461 ccaccgtgcc catgccgcac caggtccaga tgcaactgat caagcgccgc ctcgtcggca
    14521 aggagggaag cgcgcgcgtc aaggagctcc gcgccatcct ggccgacctg cccggctaca
    14581 agaacggccc ttacgccgac atccgcgcgt gggtggcggg acagatcgag cagacccggg
    14641 tgcgctctcg ggtggtccat cgcgactcga tcgccgtgcg tcgcgagggc caggcgcaga
    14701 tcgcgctggt gggccccccg aacgtgggca agtcgtcgct gctgcaggcg ctctcggaca
    14761 tccagatccg caccggcgac tatccgttca ccacgctccg gccggtcgcc gcggtcgccg
    14821 tgatccacgg gatcccggtc cagctggtgg agatccccgg gctcatcgag ggtgccacca
    14881 aggaccgggg cggcggccgg gcgctgctcg gcgtcctgcg cggtgccgac gccatcgtgt
    14941 tctgccacga tgcgacggcg ccgctccagc ccctgcggca ggtcctggcc gaggtgcgcg
    15001 ccgccgggat cgaccggccc gcgctcctga ccgccaccaa gctggatgag gccgggacct
    15061 cggcgctgga gcggctccga gcaacggtgc cccagctgcc ggtgatgggc gtctcgatcc
    15121 tcgacgacgc gagcctcgat gccttccgtg aggccgtgtg gggcttgacc ggcctggtcc
    15181 gcgtgttcac tcgccgtccc ggctcccccc aggccgaccc gctcgccgtg cgggcgggcg
    15241 ccaccgtgga ggacgtcgcc gaacggctcc acggcgagct cgccggatcg tgtcggggag
    15301 cgcgggtctg gggcccatcg gggcgcttcc ccggccagcg cgtggggcgc gaacacgcgg
    15361 tgctcgaggg tgacgtgatc gaggtgctcg cctgagccct cccgcgtgag cctcacagga
    15421 ccaggtcggc cagcaggtcg acctggtcca cctcgggcac gctggggacg aagaccagct
    15481 cgtcgatctc cagtcggcgg tacgcctcca cccggtcccg gacctgctcg gcggtccgcg
    15541 cggcgccggt cgcgatggcc tgggctcgat cgcccacgta cccgtagtac gcgaggagct
    15601 gacggcggga ctcctcgtcc cgctcgtcca ggccgaagta ggccaggcac accgtccttg
    15661 gcgtgcgggt cccgccgacc tgccggaacg cccccttgaa cgccgccagc atcccctccg
    15721 cggcctctgc cggcgcgccg cccaccgtgt acccgccctc ccagcgcgcc gcgcggcgcg
    15781 cggcccggac ggggtccccg ccgaacagca cggggatccg tccgcggagc gtcgccggcc
    15841 cgaacggggt cgggccgagg gccgagcctt ggccggccca gctctcgtgc aggcgcccga
    15901 gctgctcgtc gaagatcctc ccccgggcgt ggaagtcgcg ccccacggcc gggaagtcat
    15961 ccggccgagc ccccaccccc aggcccaggg tgagccggcc agcggagagg gcgtccacgc
    16021 tcgcggtgag cttggccaac cgtgccgcat cgtaggtggg cgcgagcagg acatcggtga
    16081 gcaggccgat cctcgaggtg accgccgcgg cggcggccag cgcggccagc tcgtcgaagc
    16141 ccggccaggc cacccggccg atggtggcca gggacgagaa gccccgagcc tcggcccggc
    16201 gggcccactc cagcagggtg ggcccctcca tgtccaacag cgtgttcccg atcccgatcg
    16261 cgatgtccac cgttccctcc tgcagcgcaa ggaagccctg cggccaccct acccgggatc
    16321 cggcctccga cgccgacccg aggtgccccg cgcgcgagcg aaccggcgta tcccccacaa
    16381 tggtggtgat ggctacccgg tgggccgcca cgtgtggaac ccggcccggc ggcgcccggc
    16441 tggtggtggc cgccgcggcc gtgatggtcc tgacggcctg ctcctcgaac ggcccctcat
    16501 cacccgcccc gagctcgggc gccggctcgg cgccatccac cgtgcccgcg cacgtctctc
    16561 cctcaccgct cccctccggc ccggcggcgg gagcggcgtg gaccacgtac ggaggcgatg
    16621 ccgctcggag cggcctggat cgctcctcgc cgccgatcgg ccgcgtccac cgcgtgtgga
    16681 cctcgcccgc cctggacgga gcgatctacg cgcagccgct gctgttccgt gagcgggtct
    16741 tcgtggccac ggagagcgat tccgtctacg ctctggacgc cgcctcggga caggtggcct
    16801 ggcgccgcca cctcggggag gcggtcccgc gctcggcgct tccctgcggg aacatcgatc
    16861 ccaccggcat caccggcacc ccggtgatcg atccctcgac cggggtcctg tacgcggtga
    16921 ccttcgtgca gcccggccgg catgacctgg tggcgctgga tacggccgac ggcagcgtgc
    16981 ggttccgccg accgatcgac ccccccggcc tcgatcctct ggtcgagcag cagcgccccg
    17041 cgctcaccat ctcggacggc cgcgtgtacg tagcctacgg gggcctcttc ggcgactgcg
    17101 gcgagtacca tggcgccgtg gtcgggagcg ctctggacgg gagcggctct ctgatctctt
    17161 accgggtgcc ttcgggacga gcggccggca tctgggcgcc acccggtccg accgtggtcc
    17221 ccggggacga cctgctggtg gcgaccggca acagcttctc cacgtcgagc ttcgacttcg
    17281 gcaacgccgt gatccgcttg tccccgagcc tcgacgtggt cgactggttc gcgccctcga
    17341 actggctcga cctcaaccgg ggcgacgtgg acctgggctc gacgagcccc gccctgctgc
    17401 ccgacggatt ggtgttcatc gccggcaagg aagggatcgg ctcgctgctc cgactggacc
    17461 gcctcggaca cgtgggtggg gaggtcttct ccgcgcgggt gtgcgaccgg gccttcggcg
    17521 ggaccgcggt gcgtccgccc ttcgtgtacg tgtcctgcac cggcggcgtg gtggggctgc
    17581 ggctgggctc gggaccgtcg ttccaggtgg cgtggcgggg ccccagcttc gacgccggtc
    17641 cgcccatcgt ctcggggggt gcggtctgga cggtggatct gggctcgggc gacctggtcg
    17701 ccctcgatgc cggaaccggc gatcagcgct cgaggcagcg cctgggagcg gtcgtgcact
    17761 tcaccgcgcc ggccgccggc gacgggcacg tctacgtggc ggcgacggat cgcgtggtgg
    17821 gggtcgccgg tggctagccc caaccgctcg tggcgcgatc ggctgatccc cggggcggtg
    17881 ctgggcctgg ccgcgggggg cgtggcgatc ggcgtcgcgc agttcg
//
LOCUS       DASQQQ010000159         7819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_395864, whole genome shotgun sequence.
ACCESSION   DASQQQ010000159 DASQQQ010000000
VERSION     DASQQQ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395864"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VE646_06490"
     CDS             <1..577
                     /locus_tag="VE646_06490"
                     /inference="COORDINATES: protein motif:HMM:NF020253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HZD79671.1"
                     /translation="IGRTGWTGELGYEIYLRDTSRGDELFERVLRAGEPYGIRVIAPS
                     EARRIEAGIFNYGSDMGIEDTPFHVTGLEKYVELGQEQDFIGRTALERILRQGVDRKL
                     VGIQIGGDPMSDEGALNDFWPVVADGGRVGRVTAGAWSPRLERNVGYAWVPIALAGVG
                     TSLGIESPRGVLPATVHSLPFVDPEKEIPKS"
     gene            612..785
                     /locus_tag="VE646_06495"
     CDS             612..785
                     /locus_tag="VE646_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79672.1"
                     /translation="MKMIQIPLPRAMQLRRNLTAYDGNCVALVELRTAPLLTRDARIA
                     RGAERLVEVVLVR"
     gene            782..1558
                     /locus_tag="VE646_06500"
     CDS             782..1558
                     /locus_tag="VE646_06500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZD79673.1"
                     /translation="MSDSIAFDRAADYYDRTRGLSGAAAAAQTELLVRELRGRGRALE
                     VGVGTGQVALPLASVGISLLGVDLSRPMVDRLLANAGGAAPFPLVLADAARLPLGDDA
                     FGAALTRWVLHLIPDWTAAVGEMARTVHPGGVLLISLGGYGSPRREIQNRFGELAGVS
                     VRPIGLDWDGWAELDVVLASLGASARALPEIPDHPEERMGSFLAAIGSNRYSWTWPVP
                     EDVRLRAVRELRGWVQERFGPLDRPRRSEHPIRWRAYDLP"
     gene            1696..2628
                     /locus_tag="VE646_06505"
     CDS             1696..2628
                     /locus_tag="VE646_06505"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HZD79674.1"
                     /translation="MPITPVEKIWMDGELVDWGEAKIHVLSHALHYGSGVFEGIRCYE
                     TKSGPAVWHLDEHVKRLFRSAKLYHMDIPFSHEAIGEAIKETIRANGLSSCYIRPLVM
                     RGYGEMGVNPLNAPVNVLVAVWPWGAYLGEDALERGVRVKISSWKRNDQNSLPSAAKA
                     TGQYINGVLAKTESLKAGYDEAIMLNHAGFVTDGSGENVFAVRGGVLSTPPISAGCLD
                     GITRASVMAMAQDLGHEVREANLTRTDLYNADEAFFTGTAAEVTPIQEVDDRVVGEGT
                     RGPITKEIQGTFFAATKGEIERYASWLTPVASSR"
     gene            2625..5183
                     /locus_tag="VE646_06510"
     CDS             2625..5183
                     /locus_tag="VE646_06510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HZD79675.1"
                     /translation="MSDDAAYRLPRRVTPVRYDLVLEPDLVAGTFAGWEDVAVHVHQP
                     ADAILLNALDLQIERGWLASEDARVEVREIRMEPETERVHLALDREVSAGAWTLHVEF
                     RGTLNDKLHGFYRSTYTDAEGPHVIATTHFEATDARRAFPCWDEPDLKAVFGVTMIVP
                     EALTAVSNGPEVDRTPVDGGKVKVRFADTIPMSTYLVAFVVGRLELTDPVDVDGVPLR
                     IAHVPGKGHLTRFALQAGAFSLRFFSEYYGIPYPDTKVDFIALPDFGAGAMENLGCIT
                     FREALVLVDPDQATQQELLNIADVVAHELAHMWFGDLVTMRWWNGIWLNEAFATFMAN
                     VTVDAFRPEWERWASFRRSISGALEIDGLDSTRSIEYPVRSPDDAADMFDTLTYTKGA
                     AVLRMLEQYLGPERFREGIRLYLDRHRLGNTETGDLWSAIEEATGEPVRRIMDSWIWQ
                     GGYPVVTAKHSDGAIVLSQERFRYLGGDDGTRWDVPMLVRFGAAQADAKPVLVGRPPS
                     VDGPTAVPVPGSAAEPRRVVANAGGSSFVRIRYGDGLRRALTESPQELSPIERYGLID
                     DAWATVVAGLSPVSDFLTLVRGFTEESDVHVWQAVIHGLAWLDRFAEGDVRERLRAYV
                     RDLVSPALDRLGWEPDPGEPDLTRALRGSLAGALAVLGHDAGAVDRLRGIEESARSGA
                     RIDAALASTAVGVVAATGGLEAYEAYLIHRDAAPTPQEQLRYLYALPDFREAELMDRT
                     LELAMTDAVRTQNAPFLLSRSIANRDLGQRAWRFVRERWGQVNDRFPENTIIYVADPV
                     RLLTTPEDEADVQAFFREHDIPQARKMLQQVLERQRINVAFRRRAEGELPTALV"
     gene            5306..6775
                     /locus_tag="VE646_06515"
     CDS             5306..6775
                     /locus_tag="VE646_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010040759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HZD79676.1"
                     /translation="MTGGDAQVGDVTVGSVRVSTDHWIGGERVPSDDAFEDVSPIDEQ
                     VIAHVARGGRGEADAAVATAREAAPRWGGMSPKERAAVLHAIGDGVQARVPELAAVET
                     RDNGSLLRSMRDSVMPRVAHNFHFFADQLLELGHEDFEIRGHRNHVSWDPSGVAVVIT
                     PWNAPLMLGTWRLAPALATGNAVVFKPPEWAPLTASILGDIAREAGLPDGALNVVQGI
                     GEEAGAALVAHPDVDRIAFTGSVGTGRLVAQAAGANLTPVSLELGGKSPFLIFGDADL
                     EAAVNQAVNQYDNAGQVCLAGTRLLVERSIHDAFLERFLQRVSALRQGDPRDEEVDLG
                     PQITREHFERVDGFVQRAKAAGARALIGGGPNEALGGLYYRPTLFVDAPAGSEILREE
                     VFGPVLTLQPFADEDEAVALGNDTDYGLAAIVYTGDPERAERVSARLVAGTVWVNCFF
                     VRDLRAPFGGSRNSGIGREGGVRSFDFYADVKNVCMAPV"
     gene            6792..7670
                     /locus_tag="VE646_06520"
     CDS             6792..7670
                     /locus_tag="VE646_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 1,2-dioxygenase"
                     /protein_id="HZD79677.1"
                     /translation="MGEIVGAGIVSHVPTIMLPEETRREINDGREISLVEGHRRLKAE
                     VFDRLRPDTVVVCDTHWESTFEHIVTAHDRRQGRFTSHELPRGMSAIPYDMPGDPELA
                     HALARQAEGRDDTWILACEDPYLPIFYGTVNIWTYLSGGERWISLAINQTGQSEDFLL
                     LGDLLGRAVEEVDRRVVILASGGMTHRFFAFRELRGHESSSPENIISPEAREADEHVL
                     EMLEKGDHRGVIDWMPEYKRFAPEGKFGHYLIMVGALGGASCTATGERFSDYEASVGT
                     GQVHVWFERPEGGWTA"
BASE COUNT         1124 a         2602 c         2906 g         1187 t
ORIGIN      
        1 gatcggcagg accgggtgga cgggcgagct gggctacgag atctacctgc gggacacctc
       61 gcgtggcgac gagctgttcg aacgtgtcct gcgtgcgggg gagccctacg ggatccgggt
      121 gatcgcgccg agcgaggccc gccggatcga ggccgggatc ttcaactacg gcagcgacat
      181 ggggatcgag gacacgccgt tccacgtcac cgggctggag aagtacgtcg agctcggcca
      241 ggagcaggac ttcatcggcc ggacggcgct cgagcggatc ctgcggcaag gggtggaccg
      301 caagctcgtc ggcatccaga tcggcggaga tcccatgagc gacgaggggg ctctcaatga
      361 cttctggccg gtggtcgcgg acggcgggag ggttggtcga gtgacggccg gggcgtggtc
      421 gccacgcctc gagcgcaacg tgggctacgc ctgggtgccg atcgccctgg ccggggtcgg
      481 aacgtcgctg gggatcgaga gccctcgcgg cgtgcttccc gcaacggtcc actccctccc
      541 gttcgtcgac ccggagaagg agatcccgaa gtcctaggct catgcagcac gcatgtgtcg
      601 atgtgcggcg catgaagatg atccagatcc cgctgccgcg agcgatgcag ctgcgtcgca
      661 acctcacggc ctacgacggt aactgcgtgg cgctggtcga gctgcgcacg gcccccctgt
      721 tgacccgcga cgcccggatc gctcgtgggg cggagcgctt ggtggaggtg gtcctggtcc
      781 gatgagcgac tcgatcgcgt tcgaccgtgc ggccgactac tacgaccgga cccgtggtct
      841 ctcgggggcc gcagccgcag cccagaccga gctgctggtg cgcgagctgc ggggtcgggg
      901 ccgggccctc gaggtgggtg tcggcaccgg ccaggtcgcg ctgcccctgg cctccgtggg
      961 gatctcgctc ctgggcgtgg acctgtctcg gccgatggtg gaccggctgc tcgcgaacgc
     1021 cggcggggcg gcccccttcc ccctggtcct ggccgatgcc gcccgccttc ccctagggga
     1081 tgacgccttc ggcgccgccc tgacgcgttg ggtgctgcac ctcatcccgg actggacggc
     1141 cgcggttggg gagatggccc ggacggtgca tccgggcggc gtcctgctga tcagcctggg
     1201 cgggtacggg tcgcctcgcc gggagatcca gaaccggttc ggggagctgg ccggcgtctc
     1261 ggtgcgcccg atcgggttgg actgggacgg ctgggccgag ctcgatgtgg tgctcgcgtc
     1321 gttgggcgct tctgcgcggg ccctgcccga gatccccgac catcccgagg agcggatggg
     1381 atcgttcctg gccgcgatcg gatcgaaccg gtactcgtgg acgtggccgg tgccggagga
     1441 cgtgcgcctc cgcgccgtcc gcgagctccg tggctgggtg caggagcggt tcgggcccct
     1501 ggaccggccc cgcaggagcg agcacccgat ccgttggcgc gcctacgacc tgccctgagg
     1561 cgcctcagcg gggccgccgc gatcgggggt ctgggcgcac cggggcggcc tccggcgtcc
     1621 ccgccacagg ctatcctttg gggcaacggg ccggcgcacg cgcccggccg gcatggatcc
     1681 gcgaaggagg ccctgatgcc catcacgccg gtcgagaaga tctggatgga cggtgagctc
     1741 gtggactggg gtgaggccaa gatccacgtc ttatcccatg ccctgcacta cgggagcggc
     1801 gtgttcgagg gcatccgctg ctacgagacg aagtccggac cggccgtgtg gcacctggac
     1861 gagcacgtga agcgcctgtt ccgctcggcc aagctctacc acatggacat ccccttctcg
     1921 cacgaggcga tcggggaggc catcaaggag acgatccggg ccaacgggtt gtcctcctgc
     1981 tacatccggc ccctggtgat gcggggctac ggtgagatgg gtgtgaaccc gctgaacgcc
     2041 ccggtgaacg tgctcgtcgc cgtctggcct tggggcgcgt acctgggcga ggacgcgctg
     2101 gagcgcgggg tccgcgtcaa gatctcctcc tggaagcgca acgaccagaa ctcgctgccc
     2161 tcggccgcca aggccaccgg gcagtacatc aacggggtcc tggccaagac ggaatccctc
     2221 aaggcggggt acgacgaggc catcatgctg aaccatgccg ggttcgtgac cgacggctcc
     2281 ggggagaacg tgttcgcggt ccggggcggc gtcctgtcca cgcccccgat ctcggccggc
     2341 tgcctggacg ggatcactcg cgcctccgtg atggccatgg cgcaggacct cgggcacgag
     2401 gttcgcgagg ccaacctcac gcgcaccgac ctgtacaacg ccgatgaggc gttcttcacg
     2461 gggaccgccg ccgaggtgac gcccatccag gaggtcgacg atcgggtggt gggggagggc
     2521 acgcgcggcc ccatcaccaa agagatccag gggaccttct tcgctgccac caagggcgag
     2581 atcgagcggt acgcctcgtg gctgaccccg gtggcgtcga gccgatgagc gacgacgccg
     2641 cgtaccggtt gccgcggagg gtcaccccag tccgctacga cctggtcctc gagcccgacc
     2701 tggttgccgg gaccttcgcg gggtgggagg acgtcgccgt ccacgtccac cagcccgcgg
     2761 atgcgatcct gctgaacgcg ctcgacctgc agatcgagcg gggctggctc gcctccgagg
     2821 acgctcgggt ggaggtccgc gagatccgga tggagccgga gaccgagcgt gtgcatctgg
     2881 ccctggaccg ggaggtctcg gccggagcgt ggaccctcca cgtcgagttc cggggaacgc
     2941 tgaacgacaa gctgcacggg ttctaccggt ccacctacac ggacgccgag ggcccgcacg
     3001 tgatcgccac cacccacttc gaggccacgg acgcgcgccg ggcgttcccc tgctgggacg
     3061 agcccgatct gaaggccgtg ttcggcgtga ccatgatcgt ccccgaggcc ctgaccgccg
     3121 tctccaacgg acccgaggtc gaccggacac ccgtcgacgg cggcaaggtg aaggtccggt
     3181 tcgccgacac gatccccatg tccacgtacc tggtggcctt cgtggtgggg cgcctggagc
     3241 tcaccgatcc ggtcgacgtc gacggcgttc ccctccggat cgcgcacgtg cccggcaagg
     3301 gccatctcac gcggttcgcc ctccaggcgg gggcgttctc gctgcgcttc ttctccgagt
     3361 actacggcat cccctatccg gacacgaagg tcgacttcat cgcccttccg gacttcgggg
     3421 ccggggccat ggagaacctg ggctgcatca cgttccggga ggccctggtg ctggtggacc
     3481 ccgatcaggc cacccagcag gagctgctca acatcgcgga cgtggtggcg cacgagctcg
     3541 cgcacatgtg gttcggggac ctggtgacga tgcggtggtg gaacggcatc tggctgaacg
     3601 aggccttcgc cacgttcatg gccaacgtca cggtggacgc gttccggccc gagtgggagc
     3661 gatgggcctc cttccgccgg tccatctcgg gcgcgttgga gatcgacggc ctggacagca
     3721 cccggtccat cgagtacccg gtgcgctcgc cggacgacgc ggcggacatg ttcgacacgc
     3781 tcacgtacac gaagggcgca gcggtcctgc ggatgctcga acagtacctg ggtccggagc
     3841 ggttccggga ggggatccgc ctgtacctgg accgtcatcg gttgggcaac acggagacgg
     3901 gggacctgtg gagcgccatc gaagaggcca ccggtgagcc ggtacgtcgg atcatggact
     3961 cctggatctg gcagggaggg tacccggtgg ttacggcgaa gcactcggat ggggcgatcg
     4021 tcctgtccca ggagcgcttc cggtacctgg gtggcgatga cggcacccgg tgggacgtcc
     4081 ccatgctggt tcggttcggg gcggcgcagg ccgatgccaa gccggtgctg gtggggcgtc
     4141 caccgtccgt ggatggcccc acggcggtcc cggtccccgg atcggccgcc gagcccaggc
     4201 gggtcgtggc caacgccggc gggtcgagct tcgtgcggat ccgctacggc gacggcttgc
     4261 ggcgggccct gaccgagtcc cctcaggagc tgtcgcccat cgagcgctac gggctgatcg
     4321 acgatgcttg ggccaccgtg gtggccggcc tgtcgccggt gagcgatttc ctgaccctgg
     4381 tccgcggctt caccgaggag agcgatgtcc acgtgtggca ggcggtgatc cacggcctgg
     4441 cctggctgga ccggttcgcc gaaggcgacg tgcgcgaacg gctccgcgca tacgtgcgcg
     4501 acctggtgag tccggccctg gaccgtctgg gctgggagcc ggatccgggt gagccggacc
     4561 tcaccagagc gctccgcggg tcgctggccg gggccctcgc cgtcctgggt cacgacgcag
     4621 gggcggtgga ccgtctgcgg ggcatcgagg agtccgcgcg ctcgggcgcc aggatcgacg
     4681 ccgccctggc ctcgacggcg gtgggggtgg tggccgccac cggtggtctc gaggcctacg
     4741 aggcgtacct gatccaccgc gacgccgcgc ccacgcccca ggagcagctg cggtacctgt
     4801 acgcactgcc ggacttccgt gaggctgagc tcatggaccg gaccctggag ctggccatga
     4861 ccgatgccgt gcggacccag aacgcgccct tcctgctctc ccgctccatc gccaaccggg
     4921 acctcggaca gcgggcctgg cggttcgtgc gagagcgctg ggggcaggtg aacgatcggt
     4981 tccccgagaa caccatcatc tacgtggccg atccggtccg gctgctcacc acgccggagg
     5041 acgaggcgga cgtgcaggcc ttcttccggg agcacgacat ccctcaggcc cggaagatgc
     5101 tccagcaggt cctggagcgt cagcggatca acgtcgcgtt ccgccgccgg gcggaagggg
     5161 agctccccac cgccctggtc tgatcggcgg gcccgggctc gccaccgcgt gcccccggcc
     5221 cctctttcgc cgagcttgac agcccgaggg gccctcaata ccgttcggca ccgaacgacg
     5281 tggcccggcc ttgacgggcc gagcgttgac cggaggcgat gcgcaggtgg gggacgtcac
     5341 ggtcggatcg gtccgggtgt ccacggacca ctggatcggc ggcgagcgcg tgccctccga
     5401 cgacgcgttc gaggacgtct cacccatcga cgagcaggtg atcgcccacg tcgcgcgggg
     5461 cgggcgggga gaggccgatg ccgccgtggc gaccgcgcgg gaggccgccc cgcggtgggg
     5521 ggggatgagc ccgaaggagc gggcggcggt tctccacgcg atcggcgatg gggtccaggc
     5581 ccgtgtcccg gagctggcgg ccgtcgagac ccgggacaac gggtcgctcc tgcgctcgat
     5641 gcgcgactcg gtcatgccgc gcgtcgccca caacttccac ttcttcgccg atcagctgct
     5701 cgagctcggc cacgaggact tcgagatcag gggccaccgg aaccacgtga gctgggatcc
     5761 ttccggcgtg gcggtggtca tcacgccgtg gaacgcgccg ctgatgctgg ggacctggcg
     5821 gttggcgccc gccctcgcga ctgggaacgc cgtcgtgttc aagccgcccg agtgggcgcc
     5881 gctgaccgcc tcgatcctcg gggacatcgc ccgcgaggcc ggcctgcccg acggagcgct
     5941 caatgtggtc caggggatcg gcgaggaggc gggcgccgcc ctggtggccc atcccgacgt
     6001 ggatcggatc gcgttcacgg gctcggtagg aaccggccgg ctggttgccc aggcggccgg
     6061 ggcgaacctc acgccggtct cgctcgagct gggcggcaag tcgccgttcc tgatcttcgg
     6121 cgacgccgac ctggaggcgg ccgtgaacca ggccgtgaac cagtacgaca acgccggaca
     6181 ggtctgcctg gccgggacgc ggctgctggt cgagcgctcg atccacgatg ccttcctcga
     6241 gcgcttcctg cagcgggtct cggcgctccg ccagggggac ccccgggatg aagaggtcga
     6301 cctcggtccg cagatcaccc gggagcactt cgagcgcgtg gacggtttcg tccaacgggc
     6361 caaggcggcc ggtgcccgtg cgctgatcgg cggcgggccc aacgaagcgc tcggcggcct
     6421 ctactaccga cccacgctgt tcgtggacgc gccggccggc tccgagatcc tccgcgagga
     6481 ggtgttcggg cccgtcctca ccctccagcc gttcgccgac gaggacgagg ccgtggcgct
     6541 cggcaacgac acggactacg ggctcgccgc gatcgtctac accggcgacc cggagcgggc
     6601 cgagcgggtg tcggcccggt tggtggccgg aacggtgtgg gtgaactgct tcttcgtgcg
     6661 ggacctgcga gcgccgttcg gcggctcccg gaactcgggc atcggccggg agggcggtgt
     6721 gcggagcttc gacttctacg cggacgtgaa gaacgtctgc atggcgccgg tctgaaagga
     6781 gatgacggcg gatgggtgag atcgtcggcg ccgggatcgt gtcccacgtc cccacgatca
     6841 tgttgccgga ggagacccgg cgcgagatca acgacggcag ggagatcagc ctggtcgagg
     6901 gccaccggcg gttgaaggcc gaggtgttcg accggctgag gcccgacacc gtggtggtgt
     6961 gcgacacgca ctgggagtcc accttcgagc acatcgtcac cgcgcacgac cgccggcagg
     7021 ggcggttcac ctcgcacgag ctgccccggg gcatgagcgc gatcccgtac gacatgccgg
     7081 gcgatccgga gctggcgcac gccctggccc ggcaggccga gggccgggac gacacgtgga
     7141 tcctggcctg cgaggatccc tacctgccga tcttctacgg caccgtgaac atctggacct
     7201 acctctccgg gggcgaacga tggatctcgc tggccatcaa ccagaccggg cagagcgagg
     7261 acttcctgct gctgggcgat ctgctgggcc gggcggtgga ggaggtggat cgtcgggtgg
     7321 tgatcctggc ctccggcggg atgacccacc ggttcttcgc gttccgcgag ctcagggggc
     7381 acgagtcctc cagcccggag aacatcatca gtcccgaggc ccgcgaggcg gacgagcacg
     7441 tgctggagat gctcgagaag ggcgaccacc ggggcgtgat cgactggatg cccgagtaca
     7501 agcggttcgc tcccgagggc aagttcgggc attacctgat catggtgggg gcgctggggg
     7561 gcgcctcctg cacggcgacg ggggagcggt tctccgacta cgaggcgtcc gtggggacgg
     7621 gccaggtcca cgtctggttc gagcgtcccg agggcggatg gacggcgtag gggcaagccg
     7681 acggaagggg ggttggaggt ggctgaggcc caggaagggt tgggctacga gcggttgggg
     7741 gagaagcggc tctcgctgac cgacgcggtc gcgcagtcgg cggggttcat ggcgccggtc
     7801 ttctcgtccg cgctgctga
//
LOCUS       DASQQQ010000160         8602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_68657, whole genome shotgun sequence.
ACCESSION   DASQQQ010000160 DASQQQ010000000
VERSION     DASQQQ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8602
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68657"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..2275)
                     /locus_tag="VE646_06525"
     CDS             complement(11..2275)
                     /locus_tag="VE646_06525"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HZD79678.1"
                     /translation="MDSREQVVQPKPERRGGLLSRLRPAPSEEGEQRESYQQLERLVR
                     GYNAKADLKPIREAYAYAEAHHEGQKRLSGEDFVEHPVAVATILADLSLDTTTLVAAL
                     LHDVVEDTDVSLDEIEEDFGAEVARIVDGLTKLMHIEFRSREQEQAENVRKMIVAMAG
                     DIRVLLIKLADRLHNMRTLGALPPARQRKTATETLEIFAPLAHRLGVQQVKWELEDLS
                     FKTLHPGPYREIASLVEARRAERQAYVEGVLRSARNRLREAGIKAEVEGRPKHLYSIY
                     EKMVVGGKEFNEIYDLAAIRILVDSVRDCYGALGAIHSLWKPVPGRFKDYIAMPKSNM
                     YQSLHTTVVGPEGRPIEVQLRTHEMHRTAEFGIAAHWRYKERQGGKKAKEAADLAWLG
                     QMMEWLKDMADPREFMEGLKIDLYRGQVFVFTPKGDVINLPAGATPVDFAYSIHTEVG
                     HRTIGAKVSGKLVPLDYELRTGDTVEILTSRAQGEGPSQDWLQFVVTPRARTKIRQWF
                     SRERREDALDQGRDALHRLMRKQNVPFKRLATEEALEQVAREMKFPNLDSLFVAVGEG
                     HVSPQSVMARLARIVSGTPEEDVTRVPLARPVQVGQPDVTSGVVVPGSVDVWVRLARC
                     CTPVPGDEIMGFVTRGQGVSVHRTDCPNARALSREPERVIEVSWRTGRPTSFVVSVQV
                     EALDRTRLLSDVATVLADHHVNILSATSTVGRDRITNLRFTFELGDIQHLSQILASVK
                     KVENVYDAYRVVPH"
     gene            complement(2309..2830)
                     /locus_tag="VE646_06530"
     CDS             complement(2309..2830)
                     /locus_tag="VE646_06530"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HZD79679.1"
                     /translation="MDGERLRGLIRDVPDFPQPGIVFKDITPLLADEIAYSTVIDLMV
                     VHFGRGNVDKVVGIEARGFILAAPVAYHFGAGFVPVRKKDKLPWDTETAEYALEYGTA
                     TLEIHKDAVTPGERVLIVDDVLATGGTAKATADLVERIGGKVIGLSFLIELDFLKGRE
                     RLEGYDLLTLLTY"
     gene            complement(2833..4011)
                     /gene="secF"
                     /locus_tag="VE646_06535"
     CDS             complement(2833..4011)
                     /gene="secF"
                     /locus_tag="VE646_06535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HZD79680.1"
                     /translation="MNVHHAIATFRGHREPRFRFMERRNIWFLVSGAVIVLSILGLAV
                     RGLNLSIDFEGGSQITYQDVKQVSVQDVQSTLADYGLAANSEVQIVGGNQVSIRTQSL
                     TSLTDTQRTQLIDRLAQQAGVNATDINRTDVGPTFGSEISRKAIQGLIVVLAAITLYI
                     SLRFEWKMAVGAMAALVHDVLITIGVYALVGREVTPATIIAVLTILGYSLYDTVVIFD
                     KIKENTESAAMLARDTYGGVVDTSLNQVFMRSVNTSLVVVFPILSLLLFGGDTLKDFA
                     FAMMIGVLSGTYSSIFVASPILTILKEREPRYAQIRARAAARSTTGRRLRSVPGPGGS
                     LPAGSDNGEGGQLTPVGTTAAATRTTARAGGSTGRPKPKGGSRSGAKGKKKPSKRKRR
                     "
     gene            complement(4008..6056)
                     /gene="secD"
                     /locus_tag="VE646_06540"
     CDS             complement(4008..6056)
                     /gene="secD"
                     /locus_tag="VE646_06540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZD79681.1"
                     /translation="MKATRNLWLSIIFVAALVTGSVAALLVGIHPSLGLDLEGGVSVI
                     LAAPQGTPKSVMERALENIRQRVDAFGVGEPLLYLSGNNIEVQIPGLARGTIQPRAKD
                     QYCLVGKDQQDFRQCFPSQAEAQKALDAIAVNPVVNSVCLTGDVWGDTAPCYPTTKDA
                     KAAIDAISVVPVAQASTAPPSPSGSFCLNGTGLPQAPCSFATKKDANAALAAVGTDVS
                     RQYCLQNADGSTLSSDQGTACFQAQDQATAALSGISAQHATQQFCAISSNGANQGCYL
                     TRDAATARLQETGQERLLQVIGTTARLQERQVLQTLSPGSAEYKATPVTCGTQAQRET
                     SACSFQALKDQTVVFLGQGPTDSATKYKLGPAKITGDMIKKATAVYNTGSQTQAATGW
                     EIDFSLTSQGAKVFGEVTSELVNQSPPLNQLAIIVDNRIISSPVVQGAITGGNGVITG
                     QFSQQRAQDLATQLNAGALPVNLKKQQVQTVSPTLGQESLHQGIIAGVVGLILLALYL
                     LFYYRMLGIVAWFGMSIWAVLAIGLVAVAGRTLGYSLTLAGVAGLVISLGITADSYIV
                     FFERLKDEVRHGKTPRAAVQPAFKRAYKTIVAADIVTALAALILYITAISSVRGFALT
                     LGVATLLDLFVVYFFKRPVVFLIARSERLVNLHGFGLTSGVAAEPEPGELVPAGGAAK
                     "
     gene            complement(6053..6775)
                     /locus_tag="VE646_06545"
     CDS             complement(6053..6775)
                     /locus_tag="VE646_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HZD79682.1"
                     /translation="MRPDGTEQGTPAPPPLATPDGGVVNLELVQANPELSAFISAADR
                     VMEALGYTEHGFRHANLTARIAYNVLTRAGFDDHTANLACVAGYLHDVGNMVSREMHG
                     QTGAVLVYHALRDEVGGQDLAAIVAAIANHEEENGYAVSPVSAAVILADKSDVHRSRV
                     RKSGQIEFDIHDRVNYAAEQSFLRVDSAAKTVTLELTIDTEISQVMEYFEIFLGRMQM
                     CRKAAGVLDCKFKLTINGAELA"
     gene            complement(6838..7209)
                     /gene="yajC"
                     /locus_tag="VE646_06550"
     CDS             complement(6838..7209)
                     /gene="yajC"
                     /locus_tag="VE646_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HZD79683.1"
                     /translation="MIQFLAQSSSTSSSGGLLSLLIPLLLMGGVFYFLLIRPQRTRAR
                     QQQSLLASLRIGDEVMTSAGIFGTIVDMDEESDTVTVEIAPGTNVHMLRRAVSQRFVE
                     EDEDEQDVDDDGTEEEADSRP"
     gene            complement(7329..8342)
                     /gene="ruvB"
                     /locus_tag="VE646_06555"
     CDS             complement(7329..8342)
                     /gene="ruvB"
                     /locus_tag="VE646_06555"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007790131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZD79684.1"
                     /translation="MTEEDRVVDPELSVEEREFDAALRPRTLEEFVGQERVKEQLGLL
                     IDGARARGETVDHLLFSGPPGLGKTTLAGIVATALGVGFQPTSGPALDRPGDLAAILT
                     NLEEGDVLFVDEIHRMPRAVEEVLYPALEDYKLDVVLGKGPSARSIRLDLPRFTLVGA
                     TTRPGRITLPLRERFGFSPRLDYYSAQDLAKIVRRSAGILQVAVEPDGADEIARRSRG
                     TPRVANRLLRRVRDFAEVRHDGTITGDVARAGLALFEVDELGLDKVDVAILSAVVEKF
                     GGGPVGLSTLAAAVGEETDTVEDVYEPYLLQLGFLKRTPRGRVATERAHRHLGVPGAL
                     PLG"
     gene            complement(8353..>8602)
                     /locus_tag="VE646_06560"
     CDS             complement(8353..>8602)
                     /locus_tag="VE646_06560"
                     /inference="COORDINATES: protein motif:HMM:NF019140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZD79685.1"
                     /translation="DVAALTLVPGVGKKVAQRVVLDLKEKLGGEGLVVEGPLADVRDA
                     LLSLGLSAQETSDAMAGLEADGGRSVEELLREALKHVG"
BASE COUNT         1247 a         3056 c         2925 g         1374 t
ORIGIN      
        1 ccacccgcat ctagtggggc acgacgcggt aggcgtcgta gacgttctcg accttcttca
       61 ccgaggccag gatctgcgac aggtgctgga tgtcccccag ctcgaaggtg aaccgcaggt
      121 tcgtgatccg gtcccggccc accgtggagg tggccgagag gatgttcacg tggtgatcgg
      181 ccagcaccgt ggccacgtcg gagagcaggc gggtccggtc cagggcctcg acctgcaccg
      241 acaccacgaa cgaggtgggg cgcccggtgc gccacgagac ctcgatcacg cgctcgggct
      301 cccgggagag cgcgcgggcg ttcgggcagt cggtgcggtg caccgacacc ccctggcccc
      361 gcgtgacgaa ccccatgatc tcgtcccccg gcaccggggt gcagcacctg gccagccgca
      421 cccacacgtc gaccgaaccg ggcaccacca ccccggaggt cacgtccggc tggccgacct
      481 ggaccggcct ggccaggggc acccgggtca cgtcctcctc gggggtgccg ctcacgatcc
      541 gggccagccg ggccatcacc gactgcggcg acacgtgccc ctcccccacc gccacgaaca
      601 gcgagtccag gttggggaac ttcatctcgc gggccacctg ctccagggcc tcctcggtgg
      661 ccaggcgctt gaacgggacg ttctgcttgc gcatcaggcg gtggagcgcg tcgcggccct
      721 ggtccagggc gtcctcccgg cgctcccggc tgaaccactg ccggatcttc gtgcgggccc
      781 gaggggtgac cacgaactgc agccagtcct gcgacgggcc ctcgccctgg gcccgtgagg
      841 tgaggatctc gaccgtgtcc ccggtgcgca gctcgtagtc caggggcacc agcttgcccg
      901 agaccttggc cccgatcgtg cggtgcccca cctcggtgtg gatcgagtag gcgaagtcca
      961 ccggcgtggc cccggcgggc aggttgatca cgtccccctt gggggtgaag acgaagacct
     1021 gtccgcggta caggtcgatc ttcagaccct ccatgaactc ccgcgggtcg gccatgtcct
     1081 tcagccactc catcatctgg ccgagccagg ccaggtccgc ggcctccttg gccttcttgc
     1141 cgccctggcg ctccttgtac cgccagtggg cggcgatgcc gaactcggcc gtgcggtgca
     1201 tctcgtgggt gcgcagctgg acctcgatcg ggcgcccctc gggccccacc acggtggtgt
     1261 gcagggactg gtacatgttc gacttgggca tcgcgatgta gtccttgaac cgcccgggga
     1321 ccggcttcca cagggagtgg atcgcgccca gggccccgta gcaatcgcgg accgagtcga
     1381 ccaggatgcg gatggcggcc aggtcgtaga tctcgttgaa ctccttgccg cccacgacca
     1441 tcttctcgta gatcgagtac aggtgcttgg ggcgcccctc gacctccgcc ttgatgccag
     1501 cctcccgcag ccggttccgg gccgagcgca ggacgccctc cacgtacgcc tggcgttcgg
     1561 cgcgccgggc ctccaccagg ctggcgatct cgcggtacgg gcccgggtgg agggtcttaa
     1621 acgacaggtc ctccagctcc cacttcacct gctggacccc caagcggtgc gcgagcgggg
     1681 cgaagatctc gagcgtctcg gtggcggtct tgcgctggcg agccgggggc agcgcgccga
     1741 gcgtgcgcat gttgtgcagg cgatcggcca gcttgatcaa caggacccgg atgtcgccgg
     1801 ccatggccac gatcatcttg cgcacgttct cggcctgctc ctgctcccgg ctgcggaact
     1861 cgatgtgcat cagcttggtg agcccgtcga cgatccgagc cacctccgcg ccgaagtcct
     1921 cctcgatctc gtcaaggctg acgtccgtgt cctccaccac gtcgtgcagg agggccgcca
     1981 ccagcgtggt ggtgtccagg ctgaggtcgg ccaggatcgt ggccaccgcg accgggtgct
     2041 ccacgaagtc ctcgcccgac aaccgcttct ggccctcgtg atgggcctcg gcgtaggcgt
     2101 aggcctcgcg gatcggtttc aggtcggcct tcgcgttgta gccacggacc aggcgctcca
     2161 gctgctggta gctctcgcgc tgctccccct cttcgctggg ggccgggcgc aggcgcgaca
     2221 gcagcccgcc gcggcgctcg ggcttgggct gcaccacctg ctcacggctg tccacgcgca
     2281 tgcctcctcg aggtagccag tgtaaggctc agtaggtgag cagcgtgagc aggtcgtagc
     2341 cctcgaggcg ctctcggccc ttcaggaagt cgagctcgat caggaaggac aagccgatca
     2401 ccttcccgcc gatccgctcc accaggtcgg ccgtggcctt ggccgtacct ccggtggcga
     2461 gcacgtcgtc gacgatcagc acgcgctccc ccggcgtgac ggcgtccttg tggatctcca
     2521 gggtggccgt cccatactcg agcgcgtact cggccgtctc cgtgtcccag ggcagcttgt
     2581 ccttcttgcg caccgggacg aagccggccc cgaagtggta ggccacgggg gccgccagga
     2641 tgaacccccg ggcctcgatg cccaccacct tgtccacgtt gccgcggccg aagtgcacca
     2701 ccatgaggtc gatcacggtg gagtaggcga tctcgtcggc gagcagcggg gtgatgtcct
     2761 tgaacacgat ccccggttgc gggaagtccg gcacgtcgcg gatcaggccc cgcagccgct
     2821 cgccgtccac cgctacctcc gcttccgttt ggacggcttc ttcttcccct tcgcgccgct
     2881 cctcgaacca cccttgggct tgggtcgccc ggtcgagccg ccggcccggg cggtggtgcg
     2941 ggtggccgcg gcggtggtgc ccaccggcgt gagctggccc ccctcgccgt tgtccgagcc
     3001 ggccggcagc gacccgcccg gcccgggcac cgagcgcaga cgccggcctg tggtggagcg
     3061 cgcggcggcc cgagcgcgga tctgcgcgta ccgaggctcg cgctccttca ggatcgtgag
     3121 gatcggcgag gccacgaaga tggacgaata ggtcccggac aggaccccga tcatcatggc
     3181 gaaggcgaag tccttcagcg tgtccccgcc gaagagcagc agcgacagga tggggaagac
     3241 caccaccagg gacgtgttca ccgagcgcat gaacacctgg ttcagggagg tgtccaccac
     3301 gccgccgtag gtgtcgcggg ccagcatggc cgcgctctcg gtgttctcct tgatcttgtc
     3361 gaagatcacc acggtgtcgt acagcgagta ccccaagatc gtcagcaccg cgatgatcgt
     3421 ggccggagtg acctcgcggc ccaccagggc gtagacgccg atcgtgatca ggacgtcgtg
     3481 caccagggcg gccatggcgc ccaccgccat cttccactcg aagcgcaggg agatgtacag
     3541 ggtgatggcc gccagcacca cgatcaggcc ctggatcgcc ttgcgggaga tctcggagcc
     3601 gaacgtgggc cccacgtcgg tgcggttgat gtccgtcgcg ttcacgccgg cctgctgcgc
     3661 cagccggtcg atgagctggg tccgctgcgt gtcggtcagg ctggtgaggc tctgggtgcg
     3721 gatcgagacc tggttgccgc cgacgatctg gacctccgag ttggcggcca gcccgtagtc
     3781 cgccagcgtg gactgcacgt cctggacgct gacctgcttg acgtcctggt acgtgatctg
     3841 ggagccgccc tcgaagtcga tcgagaggtt caggccgcgc accgccaggc ccaggatcga
     3901 cagcacgatg acggcgcccg agaccaggaa ccagatgttc cgccgctcca tgaaccggaa
     3961 gcgcggctcc cggtgacccc ggaaggtggc gatggcgtgg tggacgttca cttggccgca
     4021 cctcccgccg ggaccagctc accgggctcc ggctccgccg ccaccccgct ggtcaggccg
     4081 aagccgtgca ggttcaccag gcgctcgctc ctggcgatca ggaacaccac cgggcgcttg
     4141 aagaagtaca cgacgaacag gtccaggagg gtggccacgc ccagggtgag agcgaagccg
     4201 cgcaccgagc tgatcgcggt gatgtacagg atcagtgcgg ccagagcggt cacgatgtcg
     4261 gcagccacga tcgtcttgta cgcgcgcttg aaggccggct gcaccgcggc ccgaggggtc
     4321 ttgccgtgcc ggacctcgtc cttcagtcgc tcgaagaaca cgatgtagga gtcggcggtg
     4381 atgccgagcg agatcaccag gccggccacg cccgccagcg tcaggctgta gcccagcgtc
     4441 cggcccgcca ccgccaccag cccgattgcc agcaccgccc agatgctcat gccgaaccag
     4501 gccacgatgc cgagcatccg gtagtagaag agcaggtaca gggccagcag gatcaggccg
     4561 accacgccgg cgatgatgcc ctggtgcagc gactcctggc ccagggttgg actcaccgtc
     4621 tggacctgct gcttcttcag gttcaccggc agggcaccgg cgttcagctg ggtagccaga
     4681 tcctgggccc gctgctggga gaactgcccg gtgatcacgc cgttgccgcc ggtgatcgct
     4741 ccctgcacca ccggggagga gatgatccgg ttgtccacga tgatggccag ctggttcagg
     4801 ggcgggctct ggttcaccag ctcggaggtg acctcgccga agaccttcgc gccttgggag
     4861 gtgagggaga agtcgatctc ccaaccggtg gcggcctggg tctggctgcc cgtgttgtac
     4921 acggcggtgg ccttcttgat catgtcgccg gtgatcttgg ccggccccag cttgtacttg
     4981 gtggccgaat ccgtcggccc ctggccgagg aagaccacgg tctggtcctt cagggcctgg
     5041 aagctgcagg cggacgtctc gcgctgcgcc tgcgtgccgc aggtcaccgg ggtggccttg
     5101 tactcggcgc ttccggggct gagggtctgc aggacctggc gctcctgcag gcgggcggtg
     5161 gtcccgatca cctgcagcag ccgttcctga ccggtctcct gcagccgcgc cgtcgcggcg
     5221 tcgcgggtca ggtagcagcc ctggttcgcg ccgttcgagc tgatcgcgca gaactgctgg
     5281 gtcgcgtgtt gcgccgagat cccggacagc gcggcggtgg cctggtcctg ggcctggaag
     5341 caggccgtgc cctggtcgct ggacagcgtg ctgccgtcgg cgttctggag gcagtactgg
     5401 cgggacacgt cggtgccgac ggccgccagg gccgcgttgg cgtccttctt cgtggcgaac
     5461 gagcagggcg cctggggcag cccggtgccg ttcaggcaga agctcccgct gggtgagggc
     5521 ggcgccgtcg aggcctgggc cacggggacc acgctgatgg catcgatcgc cgccttggcg
     5581 tccttcgtgg tcgggtagca cggcgcggtg tcgccccaga cgtctccggt caggcacacc
     5641 gagttcacca ccgggttcac ggcgatggcg tcgagcgcct tctgggcctc ggcctggctg
     5701 gggaagcact gccggaagtc ctgctggtcc ttgcccacca ggcagtactg gtccttggcc
     5761 cgcggctgga tggtcccccg ggccagcccg gggatctgga cctcgatgtt gttgccggag
     5821 aggtacagga ggggctcgcc cacgccgaac gcgtcgaccc gctggcggat gttctccagg
     5881 gcccgctcca tcaccgactt gggcgtgccc tgcggggcgg ccaggatcac cgagacgccg
     5941 ccctccaggt ccagccccag gctcggatgg atgcccacca ggagcgcggc gaccgagccg
     6001 gtcaccaggg ccgccacgaa gatgatcgag agccacaggt tgcgggtcgc cttcatgcca
     6061 gctccgcccc gttgatggtg agcttgaact tgcagtccag gaccccggca gccttgcggc
     6121 acatctgcat ccggcccagg aagatctcga agtattccat gacctgactg atctcggtgt
     6181 cgatggtgag ctcgagggtc acggtcttgg cggcggagtc cacccgcagg aaggattgtt
     6241 ccgccgcgta gttgacccga tcgtggatgt cgaactcgat ctgccccgac ttgcggacgc
     6301 ggctgcggtg gacgtcggac ttgtccgcca ggatcaccgc ggccgagacg ggggatacgg
     6361 cgtagccgtt ctcctcctca tggttcgcga tagccgccac gatggccgcg aggtcttgcc
     6421 ccccgacctc atcgcgcaag gcatggtaca cgagaacggc gccggtctga ccgtgcatct
     6481 cgcgggagac catgttcccc acgtcgtgca ggtagccggc cacgcacgcc aggttcgccg
     6541 tgtggtcgtc gaacccggcc cgagtgagca cgttgtacgc gatcctggcg gtgaggttgg
     6601 cgtgacggaa gccgtgctcg gtgtagccga gggcctccat cacgcggtcg gccgccgaga
     6661 tgaacgccga cagctcgggg ttggcctgca ccagctccag gttcaccacc ccgccatcgg
     6721 gcgtggcgag cggcggtggg gccggcgttc cctgttccgt tccgtccggg cgcaccggcc
     6781 cggctcgggc tgtggtgtcc ggctccgcct tcggcgccgg ctccgctgtc cccttcttca
     6841 cggccgcgag tcggcctcct cttccgtgcc gtcgtcgtcg acgtcctgct cgtcctcgtc
     6901 ctcctccacg aaccgctggc tcaccgcgcg gcggagcatg tggacgttcg tgccgggagc
     6961 gatctcgacg gtgaccgtgt cggactcctc gtccatgtcc acgatggtcc cgaagatgcc
     7021 ggccgaggtc atgacctcgt cgccgatccg gaggctcgcc agcaggctct gctgctgccg
     7081 ggcgcgggtg cgctggggcc ggatcaggag gaagtagaac acgcccccca tcagcaggag
     7141 ggggatcagg agggacagga gcccacccga ggacgaggtg ctgctgctct gggccaggaa
     7201 ctggatcatc gaacggcgcc tttcctatgg gtccgggacg aacccgcagc atacaggcta
     7261 ccgcgcccgg gaccggacgt tcggtcctcg gttgctcgca ccggcatccc cttggggcgc
     7321 aacggccttc aacccagcgg gagggctccg ggaacgccga ggtgccgatg cgcgcgctcg
     7381 gtggccacgc gcccgcgcgg ggtccgcttg aggaacccca gctggagcag gtagggctcg
     7441 tacacgtcct ccacggtgtc ggtctcctcg cccaccgcgg ccgccagcgt ggacagcccc
     7501 accgggccgc ccccgaactt ctccaccacc gcggacagga tcgccacgtc caccttgtcc
     7561 agccccagct cgtccacctc gaacagggcc agaccggccc gagccacgtc gccggtgatg
     7621 gtgccgtcgt gccggacctc ggcgaagtcc cgcacccgcc gcagcagccg gttggccacc
     7681 cgaggggtgc cgcgggagcg ccgggcgatc tcgtcggccc cgtcgggttc caccgccacc
     7741 tgcaagatcc ccgccgagcg ccgcacgatc ttggccaggt cctgcgccga gtagtagtcg
     7801 aggcgcgggg agaagccgaa ccgctctcgc agcggcagcg tgatccggcc gggccgggtg
     7861 gtcgccccca ccagggtgaa cctcggcagg tcaagccgga tgctgcgcgc gctgggaccc
     7921 ttgcccagca ccacgtccag cttgtaatcc tccagcgccg ggtagagcac ctcctccacg
     7981 gccctcggca tccggtggat ctcgtccacg aacaggacgt cgccctcctc caggttggtg
     8041 aggatggccg ccagatcgcc gggacgatcc aaagccgggc ccgacgtggg ctggaaccca
     8101 acccccagcg cggtcgccac gatcccggcc aaggtggtct tgcccagccc gggcggccca
     8161 gagaacagca ggtggtcgac ggtctcaccc cgggcccgcg cgccgtcgat cagcagcccg
     8221 agctgctcct tgacccgctc ctggcccacg aactcttcca gggtccgggg gcgcagcgcg
     8281 gcgtcgaact cgcgctcctc caccgacagc tccgggtcca ccacccggtc ctcctcggtc
     8341 acgtcgccca ccctacccca cgtgcttgag ggcctcgcgg agcagctcct ccaccgagcg
     8401 cccaccgtcg gcctcgagcc ccgccatggc gtcactggtc tcctgggcgg acaaccccag
     8461 ggacaggagc gcgtcgcgca cgtcggccag cgggccctcc accaccagcc cctcgccgcc
     8521 gagcttctcc ttcaggtcca agaccacccg ctgcgcgacc ttcttgccca cgccgggcac
     8581 gagcgtgagc gcagccacgt cg
//
LOCUS       DASQQQ010000161        14636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_396106, whole genome shotgun sequence.
ACCESSION   DASQQQ010000161 DASQQQ010000000
VERSION     DASQQQ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14636
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396106"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VE646_06565"
     CDS             <1..456
                     /locus_tag="VE646_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HZD79686.1"
                     /translation="NAVGAVPGPMDCYLALRGLKSLAARMQAHCRNARAVADLLSGHP
                     KVRRVHYPGLTTHPGHEIAERQMRDFGGMVSFEVASREEALRMARSTRLFFLAESLGG
                     VESLIEVPAPMTHASVAGSPLEVPDTLIRLSVGIEHPDDLIADLGQALG"
     gene            546..935
                     /locus_tag="VE646_06570"
     CDS             546..935
                     /locus_tag="VE646_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79687.1"
                     /translation="MLLALVGILLVGVAGGAQRVRAGAFVALAVDARALAPGSTPRGD
                     TVVALSDRITDVDRGPGPSVWMGTDGAIYRSDPGFSPTSATPTPSPGAGTGPGLPGGV
                     AGLVTLAVLIGLLLYFRSRLNRRSGGS"
     gene            complement(932..1096)
                     /locus_tag="VE646_06575"
     CDS             complement(932..1096)
                     /locus_tag="VE646_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79688.1"
                     /translation="MQKWEYFVAPLLEHNPGEILNTFGDDGWELVSVLQLTTPAGGQS
                     LVAYLKRPKS"
     gene            complement(1099..1542)
                     /locus_tag="VE646_06580"
     CDS             complement(1099..1542)
                     /locus_tag="VE646_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZD79689.1"
                     /translation="MSQPLIEPYLPSDAELRSILGDARTVAVVGLSSKPHRESHSVAS
                     YLQRKGYRIIPVNPNEDEVLGERSYPSLLDVPQDLRVDVVDVFRRAADTPPIAAEAVR
                     RGAKVLWLQDGIVNEEARRIAEAGGLTVIMGVCIRRTSRRLSEED"
     gene            complement(1539..2108)
                     /locus_tag="VE646_06585"
     CDS             complement(1539..2108)
                     /locus_tag="VE646_06585"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HZD79690.1"
                     /translation="MDVDDLRAILAGSLAQDPRPQAPPGDRLAGVMVPILVGPEPAVV
                     FTRRTAHLSRHAGEISFPGGLQDPGERHVLDTALRETSEELGIPPSAFDVLGALAPVH
                     TTVSGILIVPFVGMLALRPTLTPNAEEIAEVLEFPVRRLIEAEAEVEVPRGDRIYRGF
                     AYGMEGHTIWGATARILHQLIEILRRRSS"
     gene            2185..3546
                     /locus_tag="VE646_06590"
     CDS             2185..3546
                     /locus_tag="VE646_06590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZD79691.1"
                     /translation="MERFDAFVVGGGGTGSEIAFRLAGAGLRVGLAERDRLGGECNHY
                     GCVPTKVMLRSAKIAHLARGGAAFGVRIPQVEVDVEAVRRRARDIIQSQSGGGAEPFE
                     RAGIRVFMQEARLTGPRRLELADGARIEARAVVLATGSEPTLPPVEGLRDGPVWTNKE
                     AIWACGSVPGSLGVIGTGAIGIEFAQIYARFGSRVAALEVMPRIMPSEDEEVSAALAP
                     ALEEEGIRLRPGVHLERALHDGRRWILEMAGGERLQVDELLVAAGRRACFDGHDLDAA
                     GVRLDEGGRPLLDATLRTTAEGIWAAGDATGELLFTHVGTYEAEIVADDILGRPRPRD
                     YHVVPRVTFCDPEVASVGLSEREARERGHEVRTGRFLMRDSERAQIEGRTDGLVKIVA
                     DPRTGQVLGGHIVAEEAGAMIHEVVAAMAARVPVEAIGRAIHAYPTLSESVKAAFARL
                     LEG"
     gene            3576..3920
                     /locus_tag="VE646_06595"
     CDS             3576..3920
                     /locus_tag="VE646_06595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HZD79692.1"
                     /translation="MDSDTATVITVTEKAARKIAQLAAKEGRSEPTLRVRVVAGGCSG
                     FSYELGFTDATAADDHVIDAYGVRVLVDPKSAPIVRGSTLDFNDSLLGGGLRMLNPQA
                     KQECACGESFSI"
     gene            4009..5196
                     /locus_tag="VE646_06600"
     CDS             4009..5196
                     /locus_tag="VE646_06600"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HZD79693.1"
                     /translation="MAPKDNTQWSDLYNEVVTTGLCTGCTACIVACPFHVLGYEDNVP
                     VQLQEDGADRCLHGDKGCSLCTLACPRFRGWEADIDEKLFGRTRKPEELIGQYQDIVL
                     ARAADPETLMHGQDGGVVSALLIWGLENGEIDGVCTSKLSEERAWDAEPTVVTDRDGV
                     LATAGSRYTYSANPLAMLKAAELGLSKLALVGMSCQASVPGSMSARRVNKWAKKVNWT
                     LGLLCSKTFSYDGLMVEIAQNELGLALDDIARVNIKGKLLFYTQEGEEITYSLKKSHR
                     FTRQGCLHCPDFSAEHADISFGGLGQEGGWSLTIIRTEKGRDIWERAVADGVVEWRPG
                     SEDPGAIDLMMKLAAKSRDRWPTTEVPESDRLPGFVGNGSGNGSGDGRAAVDAASSTA
                     SQG"
     gene            complement(5287..5616)
                     /gene="fdxA"
                     /locus_tag="VE646_06605"
     CDS             complement(5287..5616)
                     /gene="fdxA"
                     /locus_tag="VE646_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005478968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZD79694.1"
                     /translation="MTYVIAEPCIDVKDRACVDECPVDCIYEGPRMLYIHPDECVDCG
                     ACEPVCPVTAIFYEDDVPAEWKQFTAVNAEWFADNVSGVGSPGGAAQVGPSSVDHPVV
                     AGWAAAG"
     gene            complement(5692..6666)
                     /locus_tag="VE646_06610"
     CDS             complement(5692..6666)
                     /locus_tag="VE646_06610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZD79695.1"
                     /translation="MEAMDVDRRPPTGIALRDPLSWDAFVEIARTVEQTGYTALFLPE
                     ITGRDAFVALAGIATSTSTLRLGTGVVTIVSRALPSIAMGAATVHELSDGRMVLGLGT
                     GPVVPGALEQLRERVLAIKAALAGEAVKDGRGGRFRLSLDLGSEPPPVWIAALGPKAM
                     RLAGEVADGVLLNWCPPERVSFAREQIRLGARSVGRDPSAVTVAVYVRACLGQEPVHA
                     LTALRRATGQYASYPAYLRQFEQCGLGQEARAAARAVQGGDADGVPEAMVRRVCLVGE
                     PAEAMGRLQAYREAGADLPIVYPVAVLDASSSIMGTLFALAPHPAVEA"
     gene            6687..7340
                     /locus_tag="VE646_06615"
     CDS             6687..7340
                     /locus_tag="VE646_06615"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HZD79696.1"
                     /translation="MDPVRFSTGDAVSLEGELRLPADQPVASAVLCHALPIAGGSKDH
                     PILWAIRNELAARGLLVLSFNFRGVMRSGGTYGGGRTEIRDVAAGVDFVREQAPESVP
                     TLVCGWSFGANVALREAVADPRVSALALVGLPLGERAPDVPELPSAAELRAFRRPVLL
                     LAGQGDTYCPRPELELMARRLPRSEVEIVPGTDHFLWRREKQAAERIGAFAASVLRG"
     gene            complement(7337..7540)
                     /locus_tag="VE646_06620"
     CDS             complement(7337..7540)
                     /locus_tag="VE646_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79697.1"
                     /translation="MRRLLATPVGWFGFLAVEMAVLILLWRLLGRLFPGMSLRADAVV
                     LLLMGLGFTIANYAVRRRYLPPH"
     gene            complement(7537..7836)
                     /locus_tag="VE646_06625"
     CDS             complement(7537..7836)
                     /locus_tag="VE646_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79698.1"
                     /translation="MGTIALSLLLFGLLVVGTAVALSVTGRLPARSSTGGEGRGPARP
                     WWGNPLVWLGVMAGSLLLGGVVAPKLLGGVVLFLPFFWVRGLRRRPGPESRERHP"
     gene            complement(7933..9360)
                     /locus_tag="VE646_06630"
     CDS             complement(7933..9360)
                     /locus_tag="VE646_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016909839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZD79699.1"
                     /translation="MTTKPFLVAGEWRTGEGTFEVKSPFDESVVAELGVPSEADVEEA
                     TATAVKTFEQFQHLPVHARSEALDHISKRLAESVDENAELIAREGGKPVKWAKVEAVR
                     AISTFRWASEVLRHGDDEVMRLDTEASLGSRLGIVRRFPFGPVLGITPFNFPLNLVAH
                     KVAPALAVGAPIVVKPAHATPLGAIRLAELFDETDLPKGMYQVLPVSSKVADGMARDP
                     RYRKISFTGSSEIGWYLKGLDPKKRVTLELGGNAGVIVHSDADLDHAAKRIAVGGYYQ
                     AGQSCISVQRVLVQSEVFDDFAARLTKEVESLKYGDPMDPSVDVGPVIDRDNVARIEE
                     WVKEAVEQGAELLTGGRADGPFYVPTLLSQVKPEMKVCREEIFGPVVTISPYQTFDEA
                     IAEVNNSRFGLQAGVFTNDLERAFQAHRQIQTGGVVINDQSAFRADQMPYGGSKDSGF
                     GREGLRYAMEEMTEPRIMVISNIPL"
     gene            complement(9403..10848)
                     /locus_tag="VE646_06635"
     CDS             complement(9403..10848)
                     /locus_tag="VE646_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HZD79700.1"
                     /translation="MATEHETWGMYIGGEWVESGRGESEPDINPATGEAVATVQVGTR
                     EDGDRAAEAAQKAFDEVWYDTPPKERSAMMLALADAMEKDADNLARLEAEDVGKPISV
                     SAADIPFIIDNLRFFAGAARILEGKSAGEYEKGFTSMIRREPLGVTVGICPWNYPLMM
                     AIWKVGPALAAGNTSILKPAELTPRTSLRLAQLSEGILPPGVLNVITGHGSEIGDQLV
                     KHEKVRLVSVTGDTATGKVIAANAGASNVKRVHLELGGKAPVVVFDDADVEKLVENLK
                     FASFTNTGQDCTASCRVVAGPGIYDDLLSELVPAVETIKVGDIMDESIEMGPLVSPGQ
                     RDSVAGFVDRAAQAGGKVMTGGDRMEGPGYYYKPTVVADVEQTAEIVQKEVFGPVVTV
                     QRFSDEDQAVAWANDVVYGLASSVWTRDAARAMRVARRLQFGAVWVNTHFMITAEMPH
                     GGYKESGYGKDMSMYSMEDYTQIKHVMWSLD"
     gene            complement(11001..12542)
                     /locus_tag="VE646_06640"
     CDS             complement(11001..12542)
                     /locus_tag="VE646_06640"
                     /inference="COORDINATES: protein motif:HMM:NF019520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HZD79701.1"
                     /translation="MGLTVRDILAVPGLALRLVAGEEAIDRPVRWVHASELEDPTPWL
                     KGGELLLTTGAGVGATPARQRAYLRRLSAAGLAGLGFGLGFSHDRVPRALVAAAEQSA
                     FPLFEVPYPVPFIAITEAVFTRLVAEQYDVLQRAFDAEQQLTRAVVEEEGIQGICASL
                     ARVTGGWVVLVDLHGIPVAASPEAGRQRASRLWDEVRASRPEGTGFSLTLMDHGHHVW
                     IQPVGTHGRVEAFLAVGKPEAPSQVDRIVAGHALSLFAIELAKSRAVAEAERRLQGDF
                     LGSLATGTLDPPEVLRGLARYGFPRDEPVRVLSMEGGDAEELAYAVEDQLSRAGSAYL
                     VSRGEEGVHVLLPAQSQDGEPFRAAVSERLGSEVRLGSGGAVAPSAVGRSLREARYSL
                     QVCRLEGRSSAGFEDLGTYRLLLSMSEPDALRAFADSLLAPLDAYDLEQNGELTTSLR
                     AFLQHNARWETAAGELFVHRHTLRYRMRKVEELTGRDLSSSFDRMEFWLALRARELLA
                     AQEDG"
     gene            12763..14109
                     /gene="gabT"
                     /locus_tag="VE646_06645"
     CDS             12763..14109
                     /gene="gabT"
                     /locus_tag="VE646_06645"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HZD79702.1"
                     /translation="MDIPQERKVVTEIPGPKSREWFERRAKAVPPGVANIHPVVTARA
                     SGAIIEDVDGNRLIDFATGIAVLNVGHTAPEVVRAAQRQMELDTHTCFHVTANEPYIE
                     LAERLNALAPGDFEKRTMFANSGAEAVENAVKIARHATGRPAVITFDHAFHGRTLLAM
                     SLTAKVMPYKQGFGPFPPEIYRLPYAYPYRCPTGGTEQTCAESCLAYALDEMHKHIGE
                     QNIAAVIVEPIQGEGGFVVPAPGFLKGIADFCREHGIVFIADEIQSGMGRAGHWFAIE
                     AEGLVPDMITTAKSLGAGFPISAITGRADLMDAVHVGGLGGTYGGNPVAAAAALAVLD
                     KIEREDLLERSRRLGASMLERLKDMRARNVVVGDVRGRGMMTAIELVADRQTKQPIDG
                     PTGNAIVRACLEQGLVILKAGTYDNVIRLLPPLTIEEGLLTEGLDILESALADVTK"
     gene            14234..14636
                     /locus_tag="VE646_06650"
                     /pseudo
     CDS             14234..14636
                     /locus_tag="VE646_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013914889.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
BASE COUNT         2206 a         5262 c         5069 g         2099 t
ORIGIN      
        1 aacgccgtgg gcgccgtccc cgggccgatg gactgctacc tcgcgctccg ggggctgaag
       61 agcctggcgg ctcggatgca ggcccactgc cgcaacgccc gtgccgtcgc ggatctcctc
      121 tccggccatc cgaaggtccg acgggtgcac tacccgggtc tgacgacgca tccggggcac
      181 gagatcgccg agcggcagat gcgggacttc ggtggcatgg tctcgttcga ggtggcctcc
      241 agggaggagg cgctccgcat ggccaggtcc acccggctgt tcttcctggc cgagtcgctg
      301 ggaggcgtgg agtccctgat cgaggtcccc gcccccatga cccacgccag cgtcgccggg
      361 tcgcccctcg aggtgccgga cacgctgatc cgcctgtccg tcggcatcga gcaccccgat
      421 gatttgatcg ccgacctcgg ccaggccctg gggtgagggt ccaagcgagg agggccccgt
      481 ctccgccggt gggtgagccg acgggcgctc cgaccgggtc cctctccccc ctccgcgctt
      541 cggcggtgct gctcgcgctg gtggggatcc tcctcgtggg ggtcgcgggc ggggcgcagc
      601 gggtcagggc cggggcgttc gtcgccctgg cggtcgatgc cagggccctc gcaccaggga
      661 gcacgccaag aggggatacg gtcgtggccc tgtccgaccg gatcaccgat gtcgatcgcg
      721 gaccaggccc ttccgtgtgg atgggcaccg atggggcgat ctaccgctcg gacccgggct
      781 tctcgccgac ctcggcgacg cccactccct cgccgggcgc aggcaccggg ccgggccttc
      841 ccggcggcgt ggcgggactg gtgacactgg ccgtcctgat cgggctcctg ctgtacttcc
      901 ggtcccgctt gaaccgccga tccggtggct cctagctctt ggggcgcttg aggtaggcca
      961 ccagcgactg cccgccggct ggggtggtca gctgcaagac cgagaccagc tcccagccgt
     1021 catcgccgaa cgtgttgagg atctcgccgg ggttgtgctc gaggagcgga gcgacgaagt
     1081 actcccactt ctgcacgatc agtcctcctc cgacaggcgc ctgctggtcc gacggatgca
     1141 cacacccatg atgacggtca ggccccccgc ctccgcgatc cgccgagcct cctcgttcac
     1201 gataccgtcc tgcagccaca gcaccttggc tccccggcgc accgcctcgg ccgcgatcgg
     1261 aggcgtgtcg gccgcccggc ggaagacgtc caccacatcg acccgcaggt cctgcggcac
     1321 atcgagcaac gaggggtagg accgctctcc caggacctcg tcctcgttcg ggttcaccgg
     1381 gatgatccgg taccccttgc gctgcaggta ggacgcgacc gaatgcgact cccggtgggg
     1441 cttggaggag aggccgacca cggcgaccgt ccgggcatcc cccaggatcg agcggagctc
     1501 ggcatccgag ggcaggtacg gctcgatcag aggctgactc acgatgacct ccttcgcagg
     1561 atctcgatca gctgatgcag gatgcgcgcg gtcgcccccc agatcgtgtg tccttccatc
     1621 ccgtaggcga acccccggta gatgcgatcc cctcgtggga cctcgacctc ggcctccgcc
     1681 tcgatcagcc gacggacggg gaactccagc acctcggcga tctcctccgc gttgggcgtg
     1741 agcgtgggcc ggagcgccag catccccacg aaggggacga tgaggatgcc ggagaccgtc
     1801 gtgtgcaccg gggcgagcgc gcccaggacg tcgaaggcgc tcggggggat gccgagctcc
     1861 tccgaggtct cccgcagggc cgtgtccagc acgtgccgct cgcccgggtc ctggagccca
     1921 ccggggaagg agatctcacc ggcgtgccgg gagaggtggg cggtgcgtcg ggtgaagacc
     1981 acggccggct ccgggcccac caggatcggg accatcacgc cggccagacg atcgccgggg
     2041 ggcgcctggg gccgcgggtc ctgcgcgagc gaaccggcca ggatcgcccg gagatcgtcc
     2101 acgtccatcc gagatcatcg tagacgagcg gacgcggatc gcccgcccgg atgacgcagg
     2161 ccggcggcgt ggttggatag cggcatggag cgcttcgacg ccttcgtcgt gggcgggggg
     2221 ggcacggggt ccgagatcgc cttccgtctg gccggcgccg gcctgcgcgt gggactggcg
     2281 gagcgggacc ggctcggcgg cgagtgcaac cactacggct gcgtccccac gaaggtgatg
     2341 ctcaggagcg cgaagatcgc gcacctagcc cgggggggag ccgcgttcgg cgtgcgcata
     2401 ccgcaggtgg aggtggacgt ggaggccgtt cgccggcgcg cccgggacat catccagagc
     2461 cagtccgggg gcggagccga gcccttcgag cgtgccggca tccgtgtctt catgcaggaa
     2521 gcgcggctga cgggacccag acggctcgag ctggcggacg gggcgcggat cgaggctcgg
     2581 gccgtcgtgc tggccacggg cagcgaaccg accctgcccc ccgtcgaggg cctgcgcgac
     2641 ggccccgtgt ggacgaacaa ggaggcgatc tgggcatgcg ggtcggtccc aggatcgctc
     2701 ggggtgatcg gcaccggggc catcgggatc gagttcgcgc agatctacgc tcggttcggg
     2761 tcgcgcgtgg cggcgctgga ggtgatgcct cggatcatgc ccagcgagga cgaggaggtc
     2821 tccgccgccc tggccccggc cttggaggag gaggggatca ggcttcggcc cggcgtccac
     2881 ctcgagcggg ccctgcacga tggacgccgg tggatcctcg agatggccgg aggagagcgt
     2941 ctgcaggtcg acgagctgct ggtggcggcc gggcgacggg cctgcttcga cgggcacgac
     3001 ctcgatgcgg cgggcgtgcg gctggacgag ggcggtcgcc ccctgctcga tgccacgctg
     3061 cgcaccaccg cggaggggat ctgggcggcc ggcgacgcca ccggggagct gctgttcacg
     3121 cacgtcggca cctacgaggc ggagatcgtg gccgacgaca tcttgggacg gccgcggccc
     3181 cgcgactatc acgtggtgcc ccgcgtgacc ttctgcgacc ccgaggtcgc gagcgtcggg
     3241 ctcagcgagc gggaggctcg ggagcggggg cacgaggtcc gcacgggacg cttcctcatg
     3301 cgcgacagcg agcgagccca gatcgagggg cggaccgacg ggctcgtgaa gatcgtggcc
     3361 gacccacgga cgggacaggt gctgggcggc cacattgtgg ccgaggaggc gggggcgatg
     3421 atccacgagg tggtggcggc catggccgct cgcgttccgg tggaggcgat cggccgggcc
     3481 atccacgcct accccacgct ctccgagtcc gtgaaggcgg cgttcgcccg cctgctggag
     3541 ggatgatgcc ggtcgacctg ggtatcctgg gagtgatgga tagcgacacc gccaccgtca
     3601 tcacggtcac cgagaaggcg gcccgcaaga tcgcccagct cgcggcgaag gaggggcgct
     3661 ccgagcccac cctgcgcgtc cgcgtggtgg ccgggggctg ctccggcttc tcctacgagc
     3721 tcgggttcac cgacgccacg gcggccgacg atcacgtgat cgatgcctac ggcgtccggg
     3781 tcctggtcga ccccaagagc gccccgatcg tgcgaggatc caccctggac ttcaacgatt
     3841 ccctcctggg cggggggctg aggatgctca acccgcaggc caagcaggag tgcgcctgcg
     3901 gcgagtcctt ctccatctga acccggcgtc acgggccgtt ggtcccgagg gtcggcgggc
     3961 gggcggcccc cggttgccgt cctgggacgc gcgctatgct tcctggcgat ggcgccgaag
     4021 gacaacacac aatggtccga cctctacaac gaggtggtca ccaccggcct gtgcaccggg
     4081 tgcaccgcgt gcatcgtggc ctgccccttc cacgtgctgg ggtacgagga caacgtcccg
     4141 gtccagctgc aggaggacgg tgccgaccgg tgcctgcatg gtgacaaggg gtgctcgctg
     4201 tgcaccctgg cctgtccgcg gttccgcggg tgggaagcgg acatcgacga gaagctcttc
     4261 ggccggaccc ggaagccgga ggagctgatc ggccagtacc aggacatcgt gctggcccgg
     4321 gccgccgacc ccgagacgct gatgcacggg caggacggcg gcgtcgtctc ggccctgctg
     4381 atctggggtc tggagaacgg cgagatcgac ggcgtctgca cctcgaagct gtccgaggag
     4441 cgggcctggg acgccgagcc caccgtggtg accgatcgcg acggggtcct ggccacagcc
     4501 ggttcgcggt acacgtactc ggcgaaccct ctggccatgc tgaaggcggc cgagctcggg
     4561 ctgtccaagc tggcgctggt ggggatgtcc tgccaggcga gcgtgcccgg ctcgatgagc
     4621 gcccggcgcg tgaacaagtg ggccaagaag gtcaactgga cgctggggct cctgtgctcg
     4681 aagaccttct cgtacgacgg cctgatggtg gagatcgcgc agaacgagct cggcctggcc
     4741 ctcgacgaca tcgcccgggt gaacatcaag ggcaagctgc tcttctacac gcaggagggc
     4801 gaggagatca cctactccct gaagaagtcg caccggttca cccggcaggg gtgcctgcac
     4861 tgcccggact tctccgcgga gcacgccgac atctccttcg gcggcttggg gcaggaaggc
     4921 ggctggagcc tcaccatcat ccgcacggag aagggccggg acatctggga gcgcgccgtg
     4981 gccgacggcg tcgtcgagtg gcgcccaggc tcggaggatc ccggggcgat cgacctgatg
     5041 atgaagctcg cggccaagtc ccgcgaccgc tggcccacca ccgaggtccc cgagtccgac
     5101 cggctgccgg gcttcgtggg gaacggcagt gggaacggca gcggggacgg acgggccgcg
     5161 gtggatgcgg catcgtccac cgcctcgcag ggctgaccca gcccgaaccg ccgggccacc
     5221 ccgaacgcga acgatcccgg gccgctgccc gggatcgctc ggtgatcgcg cttcggaagg
     5281 agccggtcag cctgccgcgg cccatccggc cacgacgggg tgatccacgg agctcggccc
     5341 cacctgggcc gctccgcccg gcgacccgac gccgctgacg ttgtcggcga accactcggc
     5401 gttgaccgcg gtgaactgct tccactcggc gggcacgtcg tcctcgtaga agatcgccgt
     5461 gaccgggcac acgggctcgc acgcgccgca gtcgacgcac tcgtccgggt ggatgtacag
     5521 catccgcgga ccctcgtaga tgcaatccac cgggcactcg tcgacgcacg cccgatcctt
     5581 cacgtcgatg caaggctctg cgatgacgta ggtcatgcct tttccccttt tccacggtgc
     5641 ggaccttgca aatccgtagt gtacgactcg gcgggccggc tgcaaacgac ctcaggcctc
     5701 gacggccggg tggggcgcca gcgcgaacag ggtgcccatg atcgaagagg aggcgtccag
     5761 cacggccacc gggtagacga tgggcaggtc ggcaccggcc tcccgatagg cctggagccg
     5821 ccccatcgcc tcggcgggct cccccaccag gcagacccgg cggaccatcg cctccggcac
     5881 gccgtccgcg tcgcccccct ggacggcgcg agccgccgcc cgcgcctcct ggcccagccc
     5941 gcactgctcg aactgtcgga ggtacgcggg gtaggaggcg tactgaccgg tggctcggcg
     6001 cagcgcggtc agggcatgca cgggctcctg gcccaggcag gcccggacgt agacggccac
     6061 ggtcacggcg gatggatccc ggcccaccga cctcgctccc aggcggatct gctcgcgggc
     6121 gaagctcacc cgttcgggcg ggcaccagtt cagcaggacc ccgtcggcga cctccccggc
     6181 cagccgcatc gccttcggcc ccaatgcggc gatccacacg gggggtggct ccgatcccag
     6241 gtccagcgac agccggaacc gaccgccccg cccgtccttc accgcctctc cggccagggc
     6301 ggccttgatc gccagcacgc gctcccgcaa ctgctcgagc gcgcccggga cgaccgggcc
     6361 ggtccccaag cccagcacca tgcgcccatc cgagagctcg tggacggtgg ccgcgcccat
     6421 cgcgatcgag ggcagagccc gcgacacgat ggtcaccacc ccggtgccca gccggagggt
     6481 ggaggtggat gtggcgatcc cggccagcgc aacgaacgcg tcgcgaccgg taatctccgg
     6541 caggaagagc gccgtgtacc ccgtctgctc gacggtccgg gcgatctcca cgaaggcgtc
     6601 ccaggacagg ggatcgcgca gggcgatccc ggtcggcggc ctccggtcca cgtccatggc
     6661 ctccatgccc actaccatcg tcccacgtgg accccgtgcg gttcagcacc ggcgatgccg
     6721 tctccctgga gggggagctt cgcctgcccg ccgaccagcc ggtcgcctcc gccgtcctgt
     6781 gtcatgccct gcccatcgcg ggcggcagca aggaccatcc catcctgtgg gcgatccgca
     6841 acgagctggc cgcgcgcggg ctgctggtgc tgtccttcaa cttccgtggc gtgatgcgct
     6901 cgggtggcac gtacggtggg ggccggacgg agatcaggga cgtggccgcc ggggtggact
     6961 tcgtccgcga gcaggccccg gagtcagtcc cgacgctcgt gtgcggctgg tccttcggag
     7021 ccaacgtcgc cctccgagag gccgtcgccg acccgcgggt ctcggcgctg gcgttggtgg
     7081 gcctgccgct cggcgagcgg gcgcccgacg tccccgaact gccctcggcg gccgagctgc
     7141 gtgccttccg ccgtcccgtg ctgctgctgg ccggccaggg agacacgtat tgcccccgtc
     7201 cggagctgga gctgatggcc cgccgactcc cgcgctccga ggtggagatc gtgccgggca
     7261 ccgatcactt cctgtggcga cgggagaagc aggcggccga gcggatcggg gccttcgccg
     7321 cctccgtgct ccggggttag tgcggaggca ggtaccgccg gcgcacggcg tagttcgcga
     7381 tggtgaaccc gagccccatc agcagcaaga ccaccgcgtc ggcccgcagg gacatgccgg
     7441 ggaacaggcg acccagcagc cgccacagca ggatcagcac ggccatctcc acggccagga
     7501 aaccgaacca cccgaccggc gtggccagca ggcgcctcac ggatgccgct cccggctctc
     7561 cggcccgggc cgccggcgga gcccccgtac ccagaagaac gggaggaaga gcacgacacc
     7621 gcccagcagc ttgggagcca ccaccccccc caggagcagg gaccccgcca tcaccccgag
     7681 ccacaccagg gggttccccc accagggccg ggccgggccg cggccctcac cccctgtgga
     7741 ggagcgggcg gggagccggc cggtgacgct cagcgcgacg gccgtgccca ccaccagcag
     7801 gccgaacagc agcagggaca gggcgatcgt tcccacgtca ccagggtagc gcgggcgctc
     7861 gcatgtgctc ccgcccggag gcccggtccg ggagggccgg cggccgcagg ccctcccgga
     7921 tccacgccgg tgctacaggg ggatgttgga gatgaccatg atcctcggct ccgtcatctc
     7981 ctccatggcg taccgcaggc cctcccgtcc gaagcccgag tccttggacc ccccgtaggg
     8041 catctggtcg gcgcggaagg ccgactggtc gttgatcacg acgccgccgg tctggatctg
     8101 acggtgagcc tggaaggcgc gctccaggtc gttggtgaac acgccggcct gcaggccgaa
     8161 ccgggagttg ttgacctcgg cgatggcctc gtcgaaggtc tggtacgggc tgatggtcac
     8221 caccgggccg aagatctcct cgcggcagac cttcatctcg ggcttgacct gcgacagcag
     8281 ggtgggcacg tagaagggcc cgtcggcccg accgccggtg agcagctccg cgccctgctc
     8341 gaccgcctcc ttgacccact cctcgatgcg ggccacgttg tcccgatcga tcaccgggcc
     8401 gacgtcgacc gatggatcca tcgggtcacc gtacttcagg ctctcgacct ccttggtcag
     8461 gcgcgcggcg aaatcgtcga acacctcgga ctgcaccagc acccgctgga cgctgatgca
     8521 gctctggccg gcctggtagt agccccctac ggcgatccgc ttcgccgcgt ggtccaggtc
     8581 cgcgtccgag tgcacgatca cgccggcgtt gccgcccagc tcgagcgtca cccgcttctt
     8641 cgggtccaga cccttcaagt accagccgat ctcggaggag ccggtgaagg agatcttccg
     8701 gtacctgggg tcccgggcca taccgtcggc caccttcgag gagaccggca gcacctggta
     8761 catgcccttc ggcaggtccg tctcgtcgaa cagctcggcc agacggatcg cccccagcgg
     8821 cgtggcgtgg gccggcttca ccacgatcgg cgcacccacc gccagcgccg gcgcgacctt
     8881 gtgggccacc aggttcagcg ggaagttgaa gggcgtgatc ccgaggaccg gcccgaacgg
     8941 gaagcgcctg acgatcccca gccgggaacc gagggacgcc tcggtgtcca accgcatcac
     9001 ctcgtcgtcg ccgtggcgca gcacctcgga tgcccagcgg aaggtggaga tggcccgcac
     9061 ggcctcgacc ttcgcccact tcacgggctt cccgccctcc ctggcgatca gctcggcgtt
     9121 ctcgtcgacc gactcggcca ggcgcttcga gatgtggtcg agggcctcgg accgcgcgtg
     9181 caccggcagg tgctggaact gctcgaaggt cttcaccgcc gtggcggtcg cctcctcgac
     9241 gtccgcctcg ctcggcaccc ccagctcggc caccacgctt tcgtcgaacg ggctcttgac
     9301 ctcgaaggtc ccctcccccg tccgccactc tccggctacc aggaacggtt tcgtcgtcat
     9361 gcttgactca ccaccatcct ctctgcccag gtggtccgtt cgtcagtcga gcgaccacat
     9421 cacgtgcttg atctgcgtgt agtcctccat ggagtacatg gacatgtcct tgccgtaccc
     9481 gctctccttg tagccgccgt gaggcatctc ggccgtgatc atgaagtgcg tgttcaccca
     9541 cacggcgccg aactgcagac ggcgagccac ccgcatggcg cgggcggcgt ccctggtcca
     9601 cacggaggag gccagtccgt acaccacgtc gttggcccag gcgaccgcct ggtcctcgtc
     9661 ggagaagcgc tggacggtga cgaccgggcc gaagacctcc ttctgcacga tctccgcggt
     9721 ctgctccacg tcggccacca ccgtgggctt gtagtagtag ccgggaccct ccatccggtc
     9781 ccccccggtc atgaccttcc ctcccgcctg ggccgcccgg tctacgaagc ccgcgaccga
     9841 gtcgcgctgg ccgggcgaga ccagcgggcc catctcgatc gactcgtcca tgatgtcgcc
     9901 gaccttgatg gtctcgaccg ccgggaccag ctcggacagc agatcgtcgt agatccctgg
     9961 gccggccacc acccggcagg aggccgtgca gtcctgaccc gtgttcgtga agctcgcgaa
    10021 cttgaggttc tccacgagct tctccacgtc ggcgtcgtcg aacaccacca ccggggcctt
    10081 gccccccagc tccaggtgca cgcgcttgac gttgctggcg ccggcgttgg ccgcgatgac
    10141 cttgccggtg gccgtgtcgc ccgtgacgga taccaggcgc accttctcgt gcttcacgag
    10201 ctggtccccg atctcggagc cgtggccggt gatcacgttc agcacccccg ggggcaggat
    10261 cccttcggac agctgggcca gccggagcga ggtacggggc gtgagctcgg cgggcttgag
    10321 gatggacgtg ttgccggcgg cgagggccgg ccccaccttc cagatcgcca tcatcagcgg
    10381 gtagttccac gggcagatgc ccacggtcac cccgagcggc tccctccgga tcatgctggt
    10441 gaagcccttc tcgtactcgc cggccgactt gccctccagg atccgcgctg ccccggcgaa
    10501 gaaccggagg ttgtcgatga tgaacgggat gtcggcggcc gagaccgaga tgggcttgcc
    10561 cacgtcctcg gcctcgaggc gagccaggtt gtcggcgtcc ttctccatgg cgtcggccag
    10621 ggccagcatc atggccgagc gctccttcgg cggcgtgtcg taccacacct cgtcgaaggc
    10681 cttctgggcg gcctcggccg cccggtcgcc gtcctcgcgc gtaccgacct ggaccgtcgc
    10741 cacggcctca cctgtggcgg ggttgatgtc cggctcggat tccccccgcc cggactcgac
    10801 ccactcgccc ccgatgtaca tgccccatgt ctcgtgctcg gtggccatgc ccacctcccc
    10861 tgtaacgcgg atgcctcgga cggatacggt gatcccggaa ctccagagta tgcccagacg
    10921 gccttcgagc gggctactcg acgttccggc tctccggcca tccaccactg tacggagggg
    10981 acaaacggga gcggaccggc tcagccatcc tcctgcgcgg cgagcagttc ccgcgcccgc
    11041 agggccagcc agaactccat ccggtcgaac gagctcgaca ggtcgcgccc ggtcagctcc
    11101 tccaccttgc gcatccggta gcggagcgtg tgccgatgga cgaagagctc gccggccgcg
    11161 gtctcccatc tggcgttgtg ttgcaggaag gctcggagcg aggtggtcag ctccccgttc
    11221 tgctccaggt cgtaggcatc gagcggcgcc aggagcgaat cggcgaaggc ccgcaaggcg
    11281 tcgggctcgg acatcgagag cagcagccgg taggtcccca ggtcctcgaa gcccgccgag
    11341 gaccgtccct ccagacgaca cacctggagg gagtagcggg cctcgcggag gctccggccc
    11401 accgccgagg gcgctaccgc accgcccgac cccaggcgga cctcggagcc gaggcgctcg
    11461 gagacggcgg cgcggaaggg ctcgccgtcc tggctctgcg ccgggaggag cacgtgcacc
    11521 ccctcctccc ccctcgagac caggtacgcg ctgcctgccc tcgagagctg atcctccacc
    11581 gcgtacgcga gctcctcggc atcgcccccc tccatcgaga gcacacgcac cggctcgtcg
    11641 cgcgggaacc cgtacctggc cagaccgcgg aggacctcag gggggtcgag cgtccccgtg
    11701 gcgagcgagc cgaggaagtc accctgcagc cgacgctcgg cctcggccac ggcgcgggac
    11761 ttggccagct cgatggcgaa gagggagagc gcgtgcccgg ccacgatccg atccacctgg
    11821 ctgggcgcct ccggcttccc caccgccagg aaggcctcga cccggccgtg cgtgcccacc
    11881 ggctggatcc acacgtggtg tccgtgatcc atcagggtca ggctgaaccc cgtcccctcc
    11941 ggccgggagg cccgcacctc gtcccacagg cgcgaggcgc gctgccggcc ggcctccggg
    12001 ctcgcggcga ccgggatgcc gtggaggtcc accagcacga cccatccccc ggtcacccgg
    12061 gccagcgagg cacagatccc ctggatcccc tcctcctcca ccaccgcccg ggtgagctgc
    12121 tgttcggcgt cgaaggcccg ctgcaagacg tcgtactgct cggccaccag ccgggtgaac
    12181 acggcctcgg tgatggcgat gaacgggacc ggatagggca cctcgaacag cgggaacgcc
    12241 gactgctcgg ccgccgcgac cagggccctc ggtacccggt cgtgggagaa gcccaggccg
    12301 aacccaaggc ccgcgagccc ggccgcagac aggcggcgca ggtaggctcg ctgcctggcg
    12361 ggggtggcgc cgaccccggc cccggtcgtg agcaggagct cgcccccctt cagccacggc
    12421 gtcggatcct ccagctcgct ggcgtggacc caccggaccg ggcgatcgat ggcctcctcg
    12481 ccggccacca gccgcagggc cagccccgga acggccagga tgtctcgaac ggtgagcccc
    12541 acggctcgga ttgtacgagg tgtacaaagc cccccgccgc actggttccg gaagcggtac
    12601 ctgctccccc gcggtgaagc accgccccgg tcctcgcccc gtcccccggc gggactggac
    12661 ggggcgtaca agcgctccgc gaaccgttgg tcgcgaggcg gcctgacagc ccggccagcc
    12721 gcggctcaga ctcgaaagca ccccggccag ggaggacgaa acgtggacat cccgcaggag
    12781 cgcaaggtcg tcaccgagat cccgggtccg aagtcccggg agtggttcga acgtcgggcc
    12841 aaggccgtcc cccccggtgt ggcgaacatc caccccgtgg tgacggcacg cgcctcgggc
    12901 gccatcatcg aggacgtcga cggcaaccgc ttgatcgact tcgccaccgg gatcgccgtc
    12961 ctgaacgtcg gccacaccgc gccggaggtc gtccgggcgg ctcagcggca gatggagctc
    13021 gacacccata cgtgcttcca cgtcaccgcc aacgagccgt acatcgagct ggcagaacgg
    13081 ctgaacgccc ttgcccccgg agacttcgag aagcgcacga tgttcgccaa ctccggcgcc
    13141 gaggcggtcg agaacgcggt gaagatcgcc cgtcacgcca cggggcggcc cgccgtgatc
    13201 acgttcgacc acgcgttcca cggccggacc ctgctcgcga tgtccctcac cgcgaaggtc
    13261 atgccgtaca agcaggggtt cggcccgttc ccgccggaga tctaccggct gccctacgcc
    13321 tacccgtacc gctgccccac gggggggacc gagcagacct gcgccgagtc ctgcctggcc
    13381 tacgccctcg acgagatgca caagcacatc ggcgagcaga acatcgccgc ggtcatcgtg
    13441 gagccgatcc agggcgaggg tggcttcgtg gtgccggcac cggggttcct gaagggcatc
    13501 gccgacttct gccgcgagca cgggatcgtc ttcatcgccg acgagatcca gtccggtatg
    13561 ggccgcgccg gtcactggtt cgccatcgag gcggaaggcc tggtgcccga catgatcacc
    13621 acggccaagt cgctcggggc cgggttcccc atctcggcca tcaccggacg ggccgacctc
    13681 atggatgccg tccacgtggg tgggctcggc ggcacctacg gggggaaccc ggtggcggct
    13741 gcggcggccc tggcggtgct cgacaagatc gagcgggagg acctcctgga gcgctcccga
    13801 cggctggggg cgtcgatgct cgagcgcctg aaggacatgc gggcgcggaa cgtggtggtg
    13861 ggtgacgtcc gcggtcgcgg catgatgacc gcgatcgaac tggtggccga ccggcagacc
    13921 aagcagccga tcgacggacc gaccggcaac gcgatcgtcc gggcctgcct ggagcagggc
    13981 ctcgtgatct tgaaggccgg gacctatgac aacgtgatcc ggctgctgcc gccgctgacg
    14041 atcgaggaag gactgttgac cgagggcctg gacatcctgg aatcggccct ggcggacgtc
    14101 acgaagtagc gggtcccgcc gcagctcgga tccacggtcg atcgccgcct cacttccgcg
    14161 ggcagcaagg acgcgagcat cggaacgcgt tggtcgacct actgagcaaa ccgatcaccg
    14221 gatccagcgc accctgagcg gcggggaccc cttgtacctg tgccactgga tgcggcagtc
    14281 gggactggcc gacctcctgc cgtcgctgcc cgcggtctag gtgggaccga gcgccggcag
    14341 catcatgatg gctcccagca tcgggaaaga cttcgtcgga agccacccac cgggggcgat
    14401 gacacgctgg ggatcgttgg ctcttcgatc ttcccgctcc ttgaccacga ggatctgccg
    14461 gacaactcca tggcccacgc agaacgatgg gccaccggga tgccggcccg cgtacgccat
    14521 cgacgatcag accgccatca aagtgatcga tgacaccgtc ggagtcgttt ccgaggggca
    14581 ctggaagctg tttgccccaa cagcaacagg cccagtcgat ccgcacgcca tcagcg
//
LOCUS       DASQQQ010000162         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_38195, whole genome shotgun sequence.
ACCESSION   DASQQQ010000162 DASQQQ010000000
VERSION     DASQQQ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38195"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VE646_06655"
     CDS             <1..563
                     /locus_tag="VE646_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008485314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZD79703.1"
                     /translation="ESFIHPFTILLSVPLALVGALAALWLTGGGLNTMSLIGVVILVG
                     IVVNDAIVKVDFINQARARGAGVRAAILEAGSARLRPIIMTTITTVLGLLPMALGIGR
                     GADLRAPLAIAVIGGLTTATALTLIVVPVAYDLVESARVRLRAFARRSDEPSPEPVGG
                     GGSAGSRVKAPAGTAYVTGGPEEDGS"
     gene            560..>2143
                     /locus_tag="VE646_06660"
     CDS             560..>2143
                     /locus_tag="VE646_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZD79704.1"
                     /translation="MIRSAIRRPVAVTMAYIAIALLGYAAWRNIPLELLPETQLPQLS
                     IQAVWEGSSPEAVESFVTSPIEGAVEQVAGVEKVTSTSQAGQAMIQVEFTRETDMDFA
                     RLELSERLAGLREDLPATARTTVIPYVPREFREQQTPFLEYTVTGPYTPEALRAHVQD
                     LIAPEITQVDGVDDVEVSGGRDRLLEIEMDPGKVQALGLSPYQVQQRIRDLEYVKEAG
                     YVDVGGLLRTVAIRHRAGSVADILQLPVLTDRGRVVRLKDVAVVHDTYEDARQHYRID
                     GFPAVAFTIHKELGTNVVRVADAVKARIAALAPLHPPGIRLILDEDESEAVKTQLSDL
                     RLRALVAAAVIFLVLLLFLGSLRTAGIVFATIAFSILITLNLIYFGGFTLNVLTLMGI
                     AMGFGLIVDNAIVVLENVYRRRRLGDAAEVAAERGAREVVLPILAATLTTVVVLVPFV
                     YLQGELQVYYVPLAIVVGFSLLASLFVAFTFIPGLASRILRRVEPDPRGRAVPQLARA
                     YAEEVVAVQRRRAERRAELW"
BASE COUNT          291 a          734 c          780 g          338 t
ORIGIN      
        1 tcgaatcgtt catccacccg ttcaccatcc tgctcagcgt gccgctcgcc ctggtcgggg
       61 cgctggccgc gctctggctg accggcggtg ggctgaacac catgagcctc atcggggtgg
      121 tgatcctcgt cgggatcgtg gtgaacgatg ccatcgtcaa ggtggacttc atcaaccagg
      181 cgcgggcccg gggggccggc gtccgagccg cgatcctgga ggccggctcc gcgcggctcc
      241 gcccgatcat catgaccacc atcaccaccg tgctcgggct cctgcccatg gccctcggga
      301 tcggccgggg cgccgacctg cgggccccgc tggcgatcgc ggtcattggc ggcctcacca
      361 ccgccaccgc gctcaccctg atcgtcgtgc cggtcgcgta cgatctcgtg gagagcgccc
      421 gggtccggct ccgggccttc gcccgccggt cggacgagcc gtcccccgag ccggtcgggg
      481 gtggaggctc ggccggctcc cgcgtgaaag ccccggcggg gaccgcctat gtcaccggcg
      541 ggcccgagga ggacgggtcg tgatccgcag cgccatccgc cgccccgtcg cggtcacgat
      601 ggcgtacatc gcgatcgcgc tgctcggcta cgcggcgtgg cgcaacatcc cgctggagct
      661 gctgccggag acccagctgc cgcagctgag catccaggcg gtgtgggagg gctcctcccc
      721 cgaagcggtg gagtcgttcg tgacctcccc gatcgagggt gcggtggagc aggtggcggg
      781 agtggagaag gtcacgtcca catcccaggc ggggcaggcg atgatccagg tcgagttcac
      841 gcgcgagacc gacatggact tcgcccgact cgagctctcg gagcgcctcg cggggctcag
      901 ggaggatctc cccgccacgg cccggacgac ggtgatcccc tacgtcccgc gggagtttcg
      961 cgagcagcag acgccgttcc tcgagtacac cgtcacgggg ccgtacacgc ccgaggcgct
     1021 ccgggcccac gtccaggacc tgatcgcgcc ggagatcacg caggtggacg gcgtcgacga
     1081 cgtggaagtg agcggcggcc gggaccggct gctggagatc gagatggacc cgggcaaggt
     1141 gcaggcgctc gggctcagcc cataccaggt ccagcagcgg atccgggacc tggaatacgt
     1201 gaaggaagcc gggtacgtcg acgtgggcgg gctgctccgc acggtggcga tccggcaccg
     1261 tgcgggatcg gtggcggaca tcctccagct ccccgtgctg acggaccggg gccgcgtcgt
     1321 gcggctgaag gacgtggccg tggtccacga tacgtacgag gacgcgcggc agcattaccg
     1381 gatcgacggg ttccccgcgg tggcgttcac gattcacaag gagctcggca ccaacgtggt
     1441 gagggtggcc gacgcggtga aggcgaggat cgccgcgctg gcgccgctcc acccgccggg
     1501 gatccggctg atcctggacg aggacgagag cgaggcggtg aagacgcagc tctccgacct
     1561 ccgcctccgg gcgctggtgg ccgcggccgt gatcttcctc gtcctcctgc tctttctggg
     1621 ctcgctccgc accgcgggca tcgtcttcgc gaccatcgcg ttctcgatcc tcatcacgct
     1681 caacctgatc tacttcggcg gcttcacgct gaacgtgctg acgctgatgg ggatcgccat
     1741 ggggttcggg ctcatcgtgg acaacgcgat cgtggtgctg gagaacgttt accgacggcg
     1801 gcgcctcggt gatgcggccg aggtggccgc ggagcggggt gcccgggagg tggtgctccc
     1861 gatcctggcg gcgacgctca ccaccgtggt cgtgctcgtc ccgttcgtct acctgcaggg
     1921 cgagctccag gtctactacg ttcccctggc gatcgtcgtc ggcttcagtc tgctggccag
     1981 cctgttcgtg gcgttcacgt tcatccccgg cctcgcgagc cggatcctgc gccgggtgga
     2041 gccggatcca cggggacggg ccgtgcccca gctcgcgcga gcctatgcgg aggaggtggt
     2101 agcggtgcag cggcgccggg ccgagcgccg ggcggagctc tgg
//
LOCUS       DASQQQ010000163         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_150828, whole genome shotgun sequence.
ACCESSION   DASQQQ010000163 DASQQQ010000000
VERSION     DASQQQ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150828"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1639)
                     /locus_tag="VE646_06665"
     CDS             complement(<1..1639)
                     /locus_tag="VE646_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HZD79705.1"
                     /translation="MTSVSVVGTGAMGGRIARRLLDAGHQVLVWNRTESRYRELVELG
                     AVPVPSPAEAARRSDVVLTMVADPEALEALTRSPDGLAAGVATTTTFIEMSTVGPAAV
                     ARLAGMLPDGTALLDAPVLGSISEAASGSLRIFVGGPDALFQRWEPLLSVFGSPIHVG
                     PLGAGAAAKLVANLTLFGVLGTLGESLALADALGLDRGVAFEVLGATPLAAQAERRRP
                     SIESGSYPPRFALRLARKDAALILGAAVQAGVELPVAEAAHRWLTEAEGEGPGDRDYS
                     AVLAEILHRSRRGPAGAITPGGMPRYAGLIIDLDGVVWLEGHEIPGAAQAIATLRDQG
                     TRVMFLTNDPKSSREVLAGRLTGLGIPAGPADVLTSAAATARLLSSSPGFVGRAAFVV
                     GAEALHQEFERAGFDLVSEDQAERAAAVVVGGHEGFGYAELQAATTAVLHGAKLFATG
                     RDALVPGPERPSPGTGAIVAAIEAATGVAATVVGKPEPFVFQIARDMLADREPVAVVG
                     DTLSSDILGAKRAGLDAILVLTGTSTERDLDRAGVRPD"
     gene            1762..>2459
                     /locus_tag="VE646_06670"
     CDS             1762..>2459
                     /locus_tag="VE646_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014050036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZD79706.1"
                     /translation="MSPVLVTGATGRVGRAVIDRLLDAGVPVRALVHRTEATATLPPN
                     IQVVTGDLTVPESLEAALTGAGAVFLVWTAPPTTAPAVVERIASFAPRVVFLSAPHRT
                     PHPFFQQPNPMAVLYAEIERLIAATGLEWTIIRPGMFASNALLWWAPAIRSEGVVRWP
                     YGAAETAPVDDRDVAAVAALTLNRDGHAGGDYVLTGPESLSQAEQVRIIAEVLGRPIS
                     FEELSPDQFRNEREG"
BASE COUNT          353 a          940 c          845 g          321 t
ORIGIN      
        1 ggtccggtcg gacccccgct cggtccaggt cacgttcggt ggacgtgccg gtgagcacga
       61 ggatcgcatc gagcccggcg cgcttggcgc ccaggatgtc cgagctcagc gtgtcgccca
      121 cgaccgccac cggctcccga tccgcgagca tgtcgcgggc gatctggaag acgaacggct
      181 cgggcttccc gaccaccgtg gctgccaccc cggtcgcggc ctcgatcgcg gcgacgatcg
      241 caccggtccc cgggctcggg cgttcgggac ccggaaccag cgcgtcccgc ccggtggcga
      301 acagcttggc gccgtgcagg accgccgtgg tcgccgcctg cagctcggcg tagccgaagc
      361 cctcgtggcc cccgaccacc accgccgccg cgcgttctgc ctgatcctcg ctcaccaggt
      421 cgaagccggc ccgttcgaac tcctggtgga gcgcctccgc gcccaccacg aacgcggccc
      481 ggcccacgaa ccccggggac gaggagagga gccgcgcggt cgccgccgcc gaggtcaaca
      541 cgtcggccgg accggcgggg atgccgaggc cggtcaaccg gccggccaac acctcgcgcg
      601 agctcttggg gtcgttggtc aggaacatca cccgggtccc ttgatcgcgg agcgtggcga
      661 tggcctgggc cgccccgggg atctcgtgcc cctcgagcca caccacgccg tccagatcga
      721 tgatcagccc tgcgtatcgg ggcatcccgc ccggggtgat cgcgcccgct ggacctcggc
      781 gggaccggtg caggatctcg gcgagcaccg cgctgtagtc ccgatcgccg gggccctcgc
      841 cctccgcttc ggtgagccag cggtgagccg cctcggcgac cggaagctcg acgcccgcct
      901 gtacggccgc gcccaggatc agtgcggcgt ccttccgcgc cagccggagc gcgaagcggg
      961 gcgggtagct cccgctctcg atcgacggcc gccgccgctc cgcctgggct gcgagcggcg
     1021 tggcgccgag cacctcgaag gccacgccgc gatcgagccc cagggcatcg gccagcgcca
     1081 gagactcccc cagggtcccc agcaccccga acagggtcag gttggccacg agcttggcgg
     1141 ccgcgcccgc gcccagcgga cctacgtgga tcggggagcc gaacaccgac agcagcggct
     1201 cccaccgctg gaagagggcg tccgggcctc ccacgaagat ccgaagagac cccgacgccg
     1261 cctccgagat gctgccgaga acgggggcgt cgagcagcgc cgtgccatcc ggcagcatcc
     1321 ccgccagccg agcgaccgcg gccggtccca ccgtcgacat ctcgatgaac gtcgtggtgg
     1381 tcgcgacacc ggcggccagg ccatccgggc tccgtgtcag ggcctcgagc gcctccgggt
     1441 cggccaccat cgtcagcacc acgtcggagc gccgtgccgc ctcggccggg ctcggtaccg
     1501 ggaccgcccc aagctccacc agctcccggt acctggactc cgtccggttc cacacgagga
     1561 cctggtgtcc ggcatcgagg agccgccgag cgatccgccc acccatggca ccggtcccga
     1621 ccaccgacac gctcgtcatg tcgtgactca tgaaagcacg cccacgccgt tcagtcgtca
     1681 gcacgggcag ggaggcgaag gtggcatgat cgacctcgtg catcgacggg cgatctcgag
     1741 gtccccacgg accggaccgg ggtgagtccg gtcctcgtca cgggggcgac cgggcgggtc
     1801 gggcgcgccg tgatcgaccg gctgttggac gcgggcgtcc cggtccgtgc cctcgtgcat
     1861 cgcaccgagg cgacggccac cctgcccccg aacatccagg tcgtcaccgg tgacctcacc
     1921 gtgcccgagt cgctcgaggc agcgctcacc ggtgccgggg cggtcttcct ggtctggacc
     1981 gccccgccga cgaccgcgcc tgcagtcgtc gagcgcatcg cgagcttcgc gccccgtgtc
     2041 gtcttcctct cagcaccgca caggaccccg cacccgttct tccagcaacc caacccgatg
     2101 gcggtgctct acgctgagat cgagcggctc atcgcggcca ccgggctgga gtggacgatc
     2161 atccggccgg ggatgttcgc atccaacgcc ttgctctggt gggcgcccgc catccgctcc
     2221 gagggggtgg tgcgctggcc gtacggcgct gccgagacgg cacccgtcga cgaccgcgac
     2281 gtggcagcgg tcgccgccct gaccctgaat cgggacggac acgccggagg ggactacgtg
     2341 ctcacgggcc ccgagtccct gagtcaggca gagcaggtgc gcatcatcgc cgaggtcttg
     2401 gggcgtccga tcagcttcga ggagctatct ccggaccagt tccggaacga gagggaagg
//
LOCUS       DASQQQ010000164         9422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_69157, whole genome shotgun sequence.
ACCESSION   DASQQQ010000164 DASQQQ010000000
VERSION     DASQQQ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9422
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69157"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VE646_06675"
     CDS             <1..584
                     /locus_tag="VE646_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79707.1"
                     /translation="LIDVSDAPSPLAARLLDHLALQAPFSPWVALYGPGRGVALFLLD
                     EIRRIVPGLEPLAHRGTALPHWDLSPDDFARFAHNVWIEIQQEVSPLGRIQGALRLSD
                     TELARLFGVRRQAVSQWLDEGLPSARLPKALAVLQVADLLERNLQPERIPAVVRSPAR
                     AYGGLSMLQMIERGHHDELLERTRASFDWAATA"
     gene            648..1121
                     /locus_tag="VE646_06680"
     CDS             648..1121
                     /locus_tag="VE646_06680"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HZD79708.1"
                     /translation="MGRSLGRLVRGRARRPLEPPGSFPVVYLCADLEVARANVLRRFE
                     GLPYGPLDLLPGRRPALAETVVPERSVVDAVSDAGCRAAGLPTTYPLDQRGTPVPRGR
                     CQPIGRTAWSQGEPGIACRSAAVGPPTRGEELAWFPDHAGPLALSSVRAFEAWFA"
     gene            1118..1708
                     /locus_tag="VE646_06685"
     CDS             1118..1708
                     /locus_tag="VE646_06685"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HZD79709.1"
                     /translation="MTSIRRCAWVPMDDPDYVQYHDREWGTPSHDDRHLFEMLVLEGA
                     QAGLSWSTILHKRDGYRAAFEGFDAGRVARFDRRRIEGLLADPGIVRNRLKVGSAVDN
                     ARRVLEVRAEHGTLDNYLWSFVDGSPVRNRWRSLDQIPAETPTSRTMSMDLKRRGFRF
                     VGPTVCYAFMQAVGMVNDHVTSCFRWAELDASTANR"
     gene            complement(1742..2890)
                     /locus_tag="VE646_06690"
     CDS             complement(1742..2890)
                     /locus_tag="VE646_06690"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD79710.1"
                     /translation="MRSALAIAGTNLRRVLRDRTATFFIFVFPFMIILAIGAVFGASS
                     TPLLGVSSAGSGPLGADLLTRLERTEGIRVRSYPDRGSLTRAVQRGQVEAGLVIPGGF
                     DREVRGGATVSLPYIARPVGAAQGGSAIVAGVVDEQAAVLRAARFGQRETGSSFDQAL
                     ARATAASREGPPVAVVERSVGGTAGTFAYGAAQELILFVFVVSLGASSMLIESRRLGV
                     SRRMLASPTSARTIVMGEGLGRFAIALFQGVLIAGVTLVLFGVDWGDPAATAAVVILF
                     ALVGTGAAMLAGSVLENAQQAGSFGVFFGLLLAALGGCMVPLEIFPPTMRTIAHLTPH
                     AWALDAFSKILGRGAGIGGVAPQLGVLALYAGVLLALATVSFRRRLTG"
     gene            complement(2887..4056)
                     /locus_tag="VE646_06695"
     CDS             complement(2887..4056)
                     /locus_tag="VE646_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD79711.1"
                     /translation="MRGSLIVAGKDLRQRMRDRSAYVIAFLVPFGLALIFSLTLANVE
                     EGSFQATFAVADRDGGQMADSFSQILRGLDFVTLRPVPTAARARDLTDAGDVDAAFVI
                     PAGFSTSAGAGRGARIEVLTSPDATVAGTVARSLASSFASDLNGIQLSVTAALGDHTA
                     NPGRAAMADLVERARAVPAVAALEQDTAESRLFSSTTFYAAGMAVFFVFFTVEFGVRG
                     LLAERESGTLARLLVAPIRPGAVLAGKALATFAVGLVSMVALVVATTLLLGAEWGDPL
                     GVALLVVSGVLAAMGLTAVVSSLARTPAQASGYASATAVALGLLGGTFFPVSQGPSFL
                     AHLSLIAPQAWMMRGFQDLATGGSVAEILPAVGAVLAFAVVLGGLGAVRAGRVRA"
     gene            complement(4058..5005)
                     /locus_tag="VE646_06700"
     CDS             complement(4058..5005)
                     /locus_tag="VE646_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020524783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD79712.1"
                     /translation="MDDAQPVLWCRDLRKAFGDLIAVDGVGFHIAAGETYGLLGPNGA
                     GKTTTISMICGLLDRDGGEVLLEGHPLDPRAVKAKAAIGYVPQELALYPDLTGRENLR
                     FFGRLYALAGRDLEARVDEVLSVIDLQERADDRVEHYSGGMQRRLNIGLGLLNRPSLL
                     VLDEPTVGVDPQSRNAILSGVERFRGLGMAVLYTTHYMEEAERLCDRVGIIDHGQLKA
                     EGTRRELVAMVGERDRVSLAATGDLDAAERTVEAVGGVEDVSRRDGGLEVVVREAHRV
                     LPAVLSAVAEAGAHATSVEIAEPNLEAVFLHLTGRALRE"
     gene            complement(5129..5845)
                     /locus_tag="VE646_06705"
     CDS             complement(5129..5845)
                     /locus_tag="VE646_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HZD79713.1"
                     /translation="MEDPRIRIRPRGPYVVSGDPPLLRTAIVKTEHREPVDWDEGPSF
                     SPPAGEYLLCRCGASPTLPFCDGSCESLEWEDALTADRGPTAERRSLFEGEDLELTDD
                     LSLCSKAGFCTNLSSNVWDLVEESDDPDAREQLVGMVQRCPSGRLVLREAPSGAELEP
                     ELEPSIGVQPSGPFWVRGMIPVESEDGLGWEVRNRMTLCRCGSSRNKPFCDGSHKVVG
                     FRDAAMPARPEGTDSALEAP"
     gene            complement(5929..7074)
                     /locus_tag="VE646_06710"
     CDS             complement(5929..7074)
                     /locus_tag="VE646_06710"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79714.1"
                     /translation="MADRIRREDLEELMEPVEGPCVSIFMPAHRGGPQTAQDPIRLKN
                     LLSQADEELRAMGLRTPEVAELLEPGRRLLEDQGFWRRQSDGLAVYLSAGELRTFRVP
                     LDLDELVIAADRFHVKPLLPMFTVDGRFFVLALSQNEVRLLQGSRFHVEEVDLRRVPE
                     DLLEAMRYEDLEKERQLHVATRGGVGAAAIFHVHGVGGEVERDLIARFFRQVDAGLWK
                     EELHDEHAPLVLAGVDFLLPMYREISRYPSIVEEGVVGNPDRFRPEELHQRAWAILEP
                     IFAQTIREDRDRYAELEPAGRTAATVPDILTAAEQGRVDVLFSATGEHLWGRVDGRVE
                     VHSDPQPGDEDLLDLAAVRTLLTSGRIHAVEPDEVPGDGPVAAILRY"
     gene            complement(7212..8069)
                     /locus_tag="VE646_06715"
     CDS             complement(7212..8069)
                     /locus_tag="VE646_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79715.1"
                     /translation="MSHPTRYRRPANVARGLKWLRHGAVDPELGFAWSRERTGMDGQR
                     PGSPVPIFAVGARGLPYDVSEELWVVELFDVARTVLFPEQRAHGGEDVFDGSERRVLG
                     LSGRLVRRVDAWSAGLAHEFAVVCALRLRERAIQALRDADDLLGSSLAPDSPAATDGD
                     RSQVDRDLLGVELSAMQNAEYLLGMWREARAAEAYGPYRAADAFAAAAAGARVTGVLT
                     YAAAAGDDRTERSGRNADLAFADERRWQAAWLRTKLGLEDMEPHVSGSRRGGDRSHRD
                     PALPGALTA"
     gene            complement(8462..9028)
                     /locus_tag="VE646_06720"
     CDS             complement(8462..9028)
                     /locus_tag="VE646_06720"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HZD79716.1"
                     /translation="MLRLRAVAVLLGALLVAACSGASAPVEVPADQLPFSLSRSPTPS
                     PGTGDQTLEITFVHGGRLQAVVRTADASVPAPEAAIRALLKGPTRSERAGGATSSVPP
                     ATQLIGIVVTDQIAEVDLSGEFQTPAQPREVLLRVAQVVWTLVGIPGVTAVRFAIDGE
                     VVPVVTDTGSSVARPVSAPDYTAVAPST"
     gene            complement(9034..>9422)
                     /locus_tag="VE646_06725"
     CDS             complement(9034..>9422)
                     /locus_tag="VE646_06725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZD79717.1"
                     /translation="DGPSLTAFTDKARVERILGNLLDNAFDHGEGRAVRVRISREGER
                     CLVGVSDEGPGLDRRDAARIFDRFYKADASRTRERGGVGLGLAIASQNAGLLGGTIDV
                     ISSPGAGATFVLKIPVGAREPAEVSG"
BASE COUNT         1350 a         3609 c         3268 g         1195 t
ORIGIN      
        1 tgctcatcga cgtgagcgac gccccctcgc ccctcgcggc ccggctgctg gaccacctgg
       61 cgctccaggc gcccttctcc ccctgggtcg ccctgtacgg ccccgggcgg ggcgtcgcgc
      121 tgttcctgct cgacgagatc cggcgcatcg tccccggtct ggagcccctg gcgcaccggg
      181 gcacggcgct cccccactgg gacctgtccc ccgatgactt cgcacggttc gcgcataacg
      241 tgtggatcga gatccagcag gaggtctcgc ccctggggag gatccagggc gcccttcgcc
      301 tgtcggacac cgagctggcg aggctgttcg gcgtgcgtcg ccaggccgtc tcacagtggc
      361 tcgacgaggg cctgccctcc gcccggttgc ccaaggctct ggccgtgctc caggtggccg
      421 acctgctcga gcgcaacctg caacccgagc ggatcccggc cgtggtcagg agcccggcgc
      481 gggcctatgg cggcctgagc atgctccaga tgatcgagcg gggccaccac gacgagctgc
      541 tggagaggac ccgagcctcg ttcgactggg cggccaccgc ctgaggcccg catgcggttc
      601 cgccacgtcc gtcgctccgg tccgtacctg cgggtcgccg acccggactg ggtcgatccc
      661 ttggacggct cgttcgcggc cgggcgcggc ggccgctgga accccccggc tcgttcccgg
      721 tggtctacct gtgcgccgac ctcgaggtgg ccagggcgaa cgtcctgcgg cggttcgagg
      781 gcctgccgta cggccccctc gacctcttgc cgggacgacg tccggcgctc gcggagacgg
      841 tggtcccgga gcggtcggtg gtggacgcgg tgagcgatgc cggatgccgg gcggcgggcc
      901 tgcccacgac gtaccccctc gaccagcgag gaacccccgt cccccggggc cggtgccagc
      961 cgatcggccg gaccgcctgg tcgcagggcg agcccggcat cgcctgccgg agcgccgccg
     1021 tgggtccgcc gacccggggc gaggagctcg cctggttccc cgaccacgcg ggcccgctcg
     1081 ccctgagctc ggtccgcgcc ttcgaggcgt ggttcgcgtg acgtcgatcc ggcgctgcgc
     1141 gtgggtgccg atggacgacc cggactacgt ccagtaccac gaccgcgagt ggggaacgcc
     1201 ctcgcacgac gaccggcacc tgttcgagat gctggtgctg gagggagccc aggccggcct
     1261 gtcctggtcc acgatcctgc acaagcggga cggctaccgg gcggcgttcg agggcttcga
     1321 cgccgggcgg gtggcccggt ttgaccggcg tcggatcgag gggctcctgg ccgaccccgg
     1381 gatcgtgcgc aaccgcctga aggtggggtc ggccgtggac aacgcccgcc gggtgctcga
     1441 ggtccgggcc gagcacggca cgctggataa ctacctctgg tccttcgtgg acgggagccc
     1501 ggtgcggaac cggtggagga gcctggacca gatcccggcg gagacgccga cctcccgcac
     1561 catgagcatg gacctcaagc gacgaggctt ccgcttcgtg ggacccacgg tctgctacgc
     1621 cttcatgcag gccgtgggca tggtgaacga tcacgtgacc tcgtgcttcc ggtgggcgga
     1681 gctcgacgcg tcgacggcga atcgctgagg ccggagggac cggtgcaccg ccccggctcg
     1741 atcagccggt gaggcgccga cggaacgaca cggtcgccag ggcgaggagc acgccggcgt
     1801 aaagcgcgag caccccgagc tggggagcga ccccgccgat ccctgctccg cgtcccagga
     1861 tcttggagaa cgcgtccagg gcccaggcgt ggggcgtgag gtgggcgatc gtgcgcatcg
     1921 tgggcgggaa gatctcgagc ggcaccatgc acccgcccag ggcggccagc agcaggccga
     1981 agaagacgcc gaaggacccg gcctgctggg cgttctcgag caccgatccc gccagcatcg
     2041 cagcaccggt ccccaccagg gcgaacagga tcaccacggc ggcggtggcc gccggatcgc
     2101 cccagtccac cccgaacagc accagcgtca ccccggcgat caacaccccc tggaacagcg
     2161 cgatcgcgaa ccgccccagc ccctcgccca tcacgatcgt ccgggccgac gtgggcgagg
     2221 ccagcatccg tcgggacacg cccagccgtc ggctctcgat cagcatggac gaggccccca
     2281 gggagaccac gaagacgaac aggatcagct cctgcgccgc gccgtaggcg aaggtgccgg
     2341 ccgtcccccc caccgagcgc tccaccaccg ccaccggggg cccttctcgg gacgcggccg
     2401 tggcccgggc cagcgcctga tcgaagctcg agcccgtctc ccgctggccg aaccgcgccg
     2461 cccggagcac cgccgcctgc tcgtccacca ctccggccac gatcgcgctg cccccctgcg
     2521 ccgcgccgac gggccgggcg atgtagggca gggacacggt ggccccgcct cggacctcgc
     2581 ggtcgaaccc gcccgggatc accagccccg cctccacctg cccccgctgc acggccctgg
     2641 tgagcgaccc gcggtccgga tagctccgca cgcggatccc ctcggtccgc tccagccggg
     2701 tcagcaggtc ggcgcccagc ggcccgctcc cggcggagga cacacccagc aggggggtgg
     2761 acgaggcgcc gaacacggcc ccgatcgcca ggatgatcat gaacgggaac acgaagatga
     2821 agaacgtcgc cgtccggtcg cgcaggaccc ggcgcaggtt cgtgcccgcg atcgccagcg
     2881 ccgacctcac gcccgaaccc tgcccgcccg gaccgccccc agcccgccca gcaccaccgc
     2941 gaaggccagc acggcgccca cggcgggcag gatctcggcc acgctgccgc cggtggccag
     3001 gtcctggaag ccgcgcatca tccaggcctg cggcgcgatc aggctgaggt gggccaggaa
     3061 gctcggcccc tgggacacgg ggaagaacgt gccgcccagc aaccccaggg ccaccgccgt
     3121 ggccgacgcg tacccgctgg cctgggccgg ggtcctggcc aggctggaca ccaccgcggt
     3181 aagccccatc gcggccagca cgcccgagac cacgagcagc gccaccccca gcgggtcgcc
     3241 ccactcggcg cccaggagca gggtggtggc cacgaccaac gcgaccatgc tgaccagccc
     3301 caccgcgaag gtggccaggg ccttgccggc cagcacggct ccggggcgga tcggcgccac
     3361 cagcaggcgc gccagggtcc cgctctcgcg ctcggccagc aggccccgga cgccgaactc
     3421 gaccgtgaag aacacgaaga acaccgccat ccccgccgcg tagaacgtgg tcgagctgaa
     3481 caggcggctc tcggcggtgt cctgctcgag cgcggcgacc gccgggacgg cgcgggcccg
     3541 ttccaccagg tcggccatcg ccgcccggcc ggggttcgcc gtgtggtccc cgagcgccgc
     3601 ggtcaccgag agctggatcc cgttcaggtc gctggcgaac gaggaggcca gggagcgagc
     3661 caccgtgccg gccaccgtcg cgtccgggct ggtgaggacc tcgatccgag ccccccggcc
     3721 ggctccggcg gaggtcgaga acccggccgg gatcacgaag gccgcgtcca cgtcgccggc
     3781 gtcggtcagg tcccgggccc gggcggcggt gggcaccggt cggagcgtga cgaagtcgag
     3841 accccggagg atctggctga acgagtcggc catctgtccg ccgtcgcgat cggccaccgc
     3901 gaaggtggcc tggaacgagc cttcctccac gttggctagg gtgaggctga agatcagcgc
     3961 cagcccgaac ggcaccagga aggcgatcac gtacgcggag cggtcgcgca tccgctgtcg
     4021 caggtccttg ccggccacga tcagcgaccc gcgcatctca ctcccgcagg gcccgaccgg
     4081 tcaggtgcag gaacacggcc tcgaggttcg gctcggcgat ctccaccgag gtggcgtggg
     4141 cgcccgcctc ggccaccgcg gacaggaccg cgggcagcac ccggtgcgcc tcccgcacca
     4201 ccacctcgag cccaccgtcg cgccggctga cgtcctccac gccacccacc gcctccacgg
     4261 tgcgctcggc cgcgtccagg tcgccggtgg ccgccaggct cacccggtcg cgctccccca
     4321 ccatggccac gagctcccgg cgcgtgccct cggccttcag ctgcccgtgg tcgatgatcc
     4381 ccacccggtc gcacaggcgc tcggcctcct ccatgtaatg cgtggtgtac aggaccgcca
     4441 tccccaggcc ccggaaccgc tccacgccgg acaggatcgc gttgcggctc tgcgggtcga
     4501 cccccacggt cggttcgtcc agcaccagca ggctgggccg gttcaagagc cccagcccga
     4561 tgttcagccg ccgctgcatg ccgcccgagt agtgctccac gcggtcgtcg gcccgctcct
     4621 gcaggtcgat caccgagagc acctcgtcca cgcgcgcctc gaggtccctg ccggccaggg
     4681 cgtagagccg gccgaagaac cggaggttct cccggccggt caggtccggg tacagcgcca
     4741 gctcctgcgg cacgtagccg atcgcggcct tggccttcac ggcgcgcgga tcgagggggt
     4801 ggccctcaag caggacctcg ccgccgtcgc gatccagcag cccgcagatc atcgagatcg
     4861 tggtggtctt gcccgccccg ttcggcccca ggagcccgta ggtctcgccg gccgcgatgt
     4921 ggaaccccac accgtccacc gcgatcaggt cgccgaacgc cttgcgcagg tcacggcacc
     4981 acagcaccgg ttgggcatcg tccacgacgc atccccctgg atcagagcct aggggggtcg
     5041 cgagccaccc cggatggggc gcctcgcccc ccaccgaggg tccgaacgcc cccggtgtgc
     5101 ggggggagct ggccgcggtt cgggagacct acggagcctc gagggcgctg tccgtgccct
     5161 ccggtcgcgc gggcatcgcg gcatcgcgga acccgaccac cttgtgggag ccgtcgcaga
     5221 agggcttgtt ccgggagctc ccgcaccggc acagcgtcat ccggttccgg acctcccacc
     5281 cgagcccgtc ctcgctctcc accgggatca tgccccggac ccagaacggg ccgctgggct
     5341 gcaccccgat cgagggctcg agctcgggct ccagctcggc gcccgacggg gcctcccgca
     5401 gcaccaggcg gcccgagggg caccgctgca ccatccccac cagctgctcg cgggcgtcgg
     5461 gatcgtcgga ctcctcgacc aggtcccaga cgttggagga caggttcgtg cagaacccgg
     5521 ccttcgagca cagcgacagg tcgtcggtga gctcgaggtc ctcgccctcg aacagcgagc
     5581 ggcgctcggc ggtgggcccg cgatcggccg tcagcgcgtc ctcccactcc agcgactcgc
     5641 acgacccatc gcagaacggc agcgtgggcg acgctccgca ccggcacagc aggtactcgc
     5701 cggcgggggg cgagaagctg gggccctcgt cccagtccac cggctcgcgg tgctcggtct
     5761 tcacgatggc cgtccgcagc agcggcggat cgccgctcac cacgtagggc ccgcggggcc
     5821 ggatgcggat cctggggtcc tccacgggcc caagggtagc ggaccgcgcg ccggcccgca
     5881 gaggtgccgg tcgctctggc tcaggagcgc aggatcagga acgcaggatc agtagcgcag
     5941 gatcgcggcg accggcccat cgccgggaac ctcgtccggc tccaccgcgt ggatccgtcc
     6001 cgaggtcagc agggtccgga ccgccgccag gtccaggaga tcctcgtcac ccggctgggg
     6061 atccgagtgg acctcgaccc ggccgtcgac ccggccccag aggtgctcgc cggtcgcgct
     6121 gaacagcacg tccacccgac cctgctccgc cgcggtgagg atgtccggga ccgtggccgc
     6181 ggtccgcccg gccggctcga gctcggcgta ccggtcccgg tcctcccgga tcgtctgggc
     6241 gaagatcggc tccaggatcg cccaggcccg ctggtgaagc tcctcggggc ggaaccggtc
     6301 cgggttgccc accacccctt cctccacgat ggaggggtac cggctgatct cccggtacat
     6361 gggcaacagg aagtccacgc cggccaggac gagcggtgcg tgctcgtcat ggagctcctc
     6421 cttccacagg cccgcgtcga cctgccggaa gaagcgggcg atcaggtccc gctccacctc
     6481 cccgcccacc ccgtggacgt ggaagatggc ggccgcaccc acgcccccac gggtggccac
     6541 gtggagttga cgctccttct ccaggtcctc gtaccgcatc gcctcgagca ggtcctccgg
     6601 cacgcgtcgc agatcgacct cctccacgtg gaaccgggag ccctggagca gccggacctc
     6661 gttctggctg agggccagga cgaagaaccg accgtcgacc gtgaacatcg gcaacagggg
     6721 cttcacgtgg aaccgatcgg cggcgatcac cagctcgtcc aggtcgaggg ggacccgaaa
     6781 cgtccggagc tcgccggcgg acaggtacac ggccagcccg tcgctctggc gccgccagaa
     6841 cccctggtcc tccaggagcc ggcgtccggg ctccagcagc tcggcgacct cgggcgtgcg
     6901 caaccccatc gcccgcagct cctcgtccgc ctgggagagg aggttcttca gccggatcgg
     6961 gtcctgcgcc gtctgcggtc cccctcggtg cgcgggcatg aagatcgaca cgcagggacc
     7021 ctccaccggc tccatcagct cctccaggtc ctcgcgccgg atccggtcgg ccatccgttc
     7081 acctcctcgc gttcggcggc cgcgctcggg gccggcctgg gacgtcaacc cacccttacc
     7141 cgatgggcat gggacctagc ggaggtaggg acggccgtcg gcggcgaccc gggggccgcc
     7201 gcgcgccggc ctcaagcggt gagggcgccg ggaagggccg ggtcccgatg ggaccggtcg
     7261 cccccacggc ggctgccgga cacatggggc tccatgtcct cgaggccgag cttggtgcgc
     7321 agccaggccg cctgccaccg ccgctcgtcg gcgaacgcga ggtccgcgtt gcgaccagat
     7381 cgctccgttc ggtcatcgcc ggcggccgcc gcgtacgtga gcacgccggt gacccgagcc
     7441 cccgccgccg cggcggcgaa cgcgtccgcc gctcggtacg gtccatacgc ctcggcggct
     7501 cgggcctcgc gccacatccc caacaggtac tcggcgttct gcatggcgga cagctccacc
     7561 ccgagcaggt ctcgatccac ctgagaccgg tcgccgtcgg tcgccgcagg gctgtcggga
     7621 gccagcgagg aaccgaggag atcgtcggca tcgcgcagag cctggatcgc gcgctcccgc
     7681 agccggaggg cacagaccac ggcgaactca tgagcgagcc ccgcgctcca ggcgtccact
     7741 cggcgcacca gccgtccgga caggcccagc acgcggcgct ccgacccgtc gaagacgtcc
     7801 tcgccgccat gcgcccgctg ctcggggaag agcacggtgc gtgcgacgtc gaacagctcc
     7861 accacccaca gctcctcgct cacgtcgtag ggcagacccc tggcccccac cgcgaagatc
     7921 ggcacagggg aaccgggtcg ctgcccgtcc atcccggtcc gctcccggct ccaggcgaag
     7981 cccagctcgg ggtccacggc gccgtggcgg agccacttca ggcctcgggc cacgttggcc
     8041 ggtcgacggt atctggtggg atgactcatc tatgctcctt cgcgtcgatc gatgctggcg
     8101 ttcgggtcat gctgctccgg tccagccggt acggctgccg tcgctcgtgg cgcaagggac
     8161 acggtgcggc cacaccgcac gcacacgaac catctcccct gcggatcggt cagatggtcc
     8221 agccgctccc cgcatgacgg acagggcgga tcgaccgccc ccctgaggcc gcctgcgggg
     8281 cgctgggcgg cgcgtccacc ggatcgcatg cctcgagtct cgccggaggc ggtttcgggt
     8341 ggtggacggg cgggttacag cttggtgacg atgcgccatc tcccccctct ggtcgtcccc
     8401 tggatcggct catggctacc cgcggtggat cggcgggaaa cggagggaga cccgagccag
     8461 atcaggtcga tggggcgacc gccgtgtagt ccggggcgct cacgggtcgg gccacgctgg
     8521 accccgtatc ggtcacgacc gggacgacct ctccgtcgat cgcgaaccgc acggcggtga
     8581 cccccgggat gcccaccagc gtccacacca cctgcgccac gcggagcagg acctcgcgcg
     8641 gctgggccgg ggtctggaac tccccggaca ggtccacctc ggcgatctga tcggtgacca
     8701 cgatgccgat caactgggtg gcgggcggaa cgctcgaggt cgcgcccccc gcccgctccg
     8761 acctggtggg gcccttcagc agggcccgga tcgcggcttc cggcgcgggg accgacgcgt
     8821 ccgccgtccg caccaccgct tggagccggc ccccgtgcac gaacgtgatc tccagggtct
     8881 gatcgccggt tcccggcgac ggcgtggggg aacggctcag ggagaacggc agctggtcgg
     8941 cggggacctc gaccggcgcc gaggcgccgg agcaggcggc caccaacagc gcgccgagca
     9001 ggaccgccac ggcacggagc cggagcatcc gcctcatccg gagacctccg ccggttcccg
     9061 cgccccgacc gggattttca ggacgaaggt ggcgccggca cccggcgagc tgatcacgtc
     9121 gatcgtgccc cccaggagcc cggcgttctg cgaagcgatc gccagcccca ggccgacgcc
     9181 accccgttcc cgcgtgcggc tggcatcggc cttgtagaag cggtcgaaga tccgggccgc
     9241 gtcccgccgg tcgaggccgg ggccctcgtc ggagaccccg accaggcagc gctcgccctc
     9301 ccgcgagatc cgcacgcgca cggcccggcc ctccccgtga tcgaaggcgt tgtccaggag
     9361 gttgcccagg atgcgttcca cccgggcctt gtccgtgaac gccgtcaggg acgggccgtc
     9421 ga
//
LOCUS       DASQQQ010000165         4467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_38397, whole genome shotgun sequence.
ACCESSION   DASQQQ010000165 DASQQQ010000000
VERSION     DASQQQ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38397"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VE646_06730"
     CDS             <1..1140
                     /locus_tag="VE646_06730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZD79718.1"
                     /translation="GSARRFHELERFHELVQSLEAVFWEADPTTLQFRFLSRRAESML
                     TGPDQDRQAPDRWGDHIDPQDREGALDAVREAIEGTVDRSVEYRARERDGKPVWIRDL
                     VHVIRDPQRDPTALRGLMVDITGRKRAEEALRQSERKYLEAFRREREATQRLRALDEM
                     KNTFLEAVSHDLRTPLTSILGSALTLEQAGLDMDPADALDLLHRIAGNARKLERLLSD
                     LLDLDRLQRGIIAPQRRPTDLGALVRHAVEDPDLLSGHPVDVDLDPVVISVDAAKVER
                     IVENMLANAGRHTPEGTRLWVRVKPADGGALVVVEDEGPGVPSELKEAVFEPFRQGQS
                     AASHSPGVGVGLSLVSRFAELHGGRAWVEDRPGGGASFRVFLPDA"
     gene            complement(1176..1547)
                     /locus_tag="VE646_06735"
     CDS             complement(1176..1547)
                     /locus_tag="VE646_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79719.1"
                     /translation="MGILDKVKEAAGGVAEVTKKGAAQVQTKVHLSQLRSRADDAAKR
                     LGYLIHKERTEGTSAAGEVEALVAEITDLERQIAEGTTAEGTTAEGTPAQGTPTQGTP
                     AQGTAPGTASSEAPAEPPAGS"
     gene            complement(1702..2229)
                     /locus_tag="VE646_06740"
     CDS             complement(1702..2229)
                     /locus_tag="VE646_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HZD79720.1"
                     /translation="MAIEEAALEVPVHEHEEHRHKKLVIIASSGDLDKAWAQMIIATA
                     GAAMEMETTIFFTFWGLFSLVKENVRVTGENWMQKMVAMMDHPGMQHLKLGKMNFVGA
                     GPAMMKKLAREHSVASPGELLQMAVDMGVKLVPCQMTMDMLGLKREDMIDGVGEPMGA
                     AGMLLEAQDAVTLFV"
     gene            complement(2243..2476)
                     /locus_tag="VE646_06745"
     CDS             complement(2243..2476)
                     /locus_tag="VE646_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014645171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HZD79721.1"
                     /translation="MSQAITKTLDLKGLSCPLPIVKTAQAMKELSSGDLIEALATDPG
                     STADFTAWCRSTGNELIEHEEDGGTFRFVIRKK"
     gene            complement(2473..3651)
                     /locus_tag="VE646_06750"
     CDS             complement(2473..3651)
                     /locus_tag="VE646_06750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HZD79722.1"
                     /translation="MPARVVVLGGGVGGTLAANLVSRELGADVAVTVVDPTGMHVYQP
                     GYLYVALGQANGRWLTRDERTLLRPDVGLLVDEAVRIHPDAGTVQLARGGGIEFDYLV
                     MATGARLAPDQIPGLEEGSYEFYSLDGAQRLREALRGFSGGRILVGIAGIPYRCPPAP
                     IEFVFMLEEYLRRRGLRERTEVTLLSPLNRAFTIEAASKLIQPIMEERGIGLTTFFNV
                     ESVDPSAGVVSSLEGEKAEYDLLVLVPPHRGQTVVSESNLGDASGWLPTDRATLQVQG
                     YERIFAIGDGTDLPISKSGSTAHFEAPVVASRIASLVRGTAPKENYGGRVMCFLETGN
                     RKATVLRFDYEHPPAPPSPNVLWHVSKWMLNRLYWGIVPKGRLPENLPLPVPTGRSES
                     "
     gene            complement(3656..3898)
                     /locus_tag="VE646_06755"
     CDS             complement(3656..3898)
                     /locus_tag="VE646_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HZD79723.1"
                     /translation="MAGVTHIDRSIDARGMPCPGPLMSLIGAIRESEVGAVIEVLSSD
                     EGSRTDIPAWIHKAGHEMVETVEDDGFARFVVRKAR"
     gene            complement(3912..4316)
                     /locus_tag="VE646_06760"
     CDS             complement(3912..4316)
                     /locus_tag="VE646_06760"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HZD79724.1"
                     /translation="MDGQTLSLVLFSGTDDKLNAAAVLTAGAAAMGRKVNLFLQYWAL
                     DAFRADRIAKDHGVAPEAGPAGAQILQRHGGQHWSEIFQQAKSIGEVAIHACSLSMEM
                     FGLTMDDLDPVVDDQEGVASFFMEVDGPVTFI"
BASE COUNT          662 a         1580 c         1495 g          730 t
ORIGIN      
        1 gggagcgctc gtcggttcca cgagctggag cgcttccacg agctcgtcca gagcctcgag
       61 gcggtcttct gggaggcgga ccccaccacc ctgcagttcc ggttcctcag tcgccgagcc
      121 gagtcgatgc tgaccggccc ggaccaagat cggcaggccc ccgaccggtg gggagaccac
      181 atcgacccgc aggaccgcga gggggccctg gatgccgtcc gagaggcgat cgaaggcacc
      241 gtcgaccgca gcgtcgagta ccgggccagg gagcgtgacg gcaagcccgt ctggatccgc
      301 gacctggtcc acgtgatccg ggacccgcag cgtgacccga ccgccctgcg ggggctcatg
      361 gtggacatca ccggacgcaa gcgggccgag gaggccctgc ggcagagcga gcggaagtac
      421 ttggaagcgt tccgtcgtga gcgggaggcc acccagcgcc tgcgggccct ggacgagatg
      481 aagaacacgt tccttgaggc cgtctcccac gacctgagaa ccccgctgac ctcgatcctg
      541 ggatccgcgc taacgctgga gcaggcgggc ctggacatgg atccggccga cgccctggat
      601 ctgctgcacc ggatcgcggg gaacgcgcgc aagctcgagc gcctgctctc cgacctgctc
      661 gatctggacc gcctgcagcg cggcatcatc gccccgcagc gtcggccgac ggatctgggg
      721 gcgctggtcc ggcacgcggt ggaggatccg gatctgctct ccggtcaccc ggtggacgtc
      781 gacctggatc ccgtggtgat ctcggtcgac gcggccaagg tggagcggat cgtcgagaac
      841 atgctggcca acgccgggcg gcacacgccg gaaggcacgc gcctgtgggt ccgggtcaag
      901 cccgccgatg gcggcgcgct cgtggtggtc gaagacgagg gtcccggcgt gccgagcgag
      961 ctcaaggagg ccgtcttcga gccattccgc cagggacaga gtgccgcttc gcactcaccc
     1021 ggcgtggggg tgggactgtc gctcgtgtcc cgcttcgccg agctgcacgg cggccgagcc
     1081 tgggtggagg atcgcccggg cggcggcgcg tccttccggg tcttcctgcc cgacgcctga
     1141 cggacccggg gccgtgtcgg cgcccgatgt gtcgatcagc tccctgccgg aggctccgcc
     1201 ggggcctcgg agctcgccgt tcccggggcg gtgccctggg cgggggtgcc ctgggtgggg
     1261 gtgccctggg cgggggtgcc ctcggccgtg gtgccctcgg ccgtggtgcc ctcggcgatc
     1321 tgccgctcca ggtccgtgat ttcggccacg agcgcctcca cctcgccggc ggccgaggtg
     1381 ccctcggtcc gctccttgtg gatcaggtac ccgagcctct tggccgcgtc gtccgctctg
     1441 ctccgcagct ggctcaggtg gaccttcgtc tggacctggg cggccccctt cttggtcacc
     1501 tcggcgacgc cgccggcggc ctctttcacc ttgtccagga ttcccatccg aaccacctcc
     1561 tcactactgg ttgcaccgta ccgcgacggc cgcccatccc gccaccccgg acagggtgag
     1621 gcccgggagg ttggtggggg actagcctcc cgggcctcgt tgtcggggtg cgatggacgg
     1681 cccgacaacc gatggccacg ctcagacgaa gagcgtgacc gcgtcctgcg cttcgaggag
     1741 catcccggcg gcgcccatgg gctcacccac gccgtcgatc atgtcctcgc gcttgagccc
     1801 caacatgtcc atcgtcatct ggcagggcac gagcttgacg cccatgtcca ccgccatctg
     1861 caggagctca ccggggctcg ccacgctgtg ctcacgggcg agcttcttca tcatcgccgg
     1921 cccggccccg acgaagttca tcttgccgag cttcaagtgc tgcatcccgg ggtgatccat
     1981 catcgcgacc atcttctgca tccagttctc cccggtgacc cggacgttct ccttgaccag
     2041 cgagaacaga ccccagaagg tgaagaagat cgtggtctcc atctccatgg cagcgcccgc
     2101 cgtggcgatg atcatctggg cccacgcctt gtccagatcg ccgctcgagg cgatgatcac
     2161 cagcttcttg tgtcggtgct cctcgtgctc gtggaccggg acctcgagcg ccgcttcctc
     2221 gatcgccatc gttttcaccc cgtcacttct tgcggatgac gaaccggaag gtgccgccgt
     2281 cttcctcgtg ctcgatcagc tcgttgcccg tcgaccgaca ccaggccgtg aagtcggccg
     2341 tggaacccgg atccgtggcc agggcctcga tcaggtcccc ggacgacagc tccttcatgg
     2401 cctgagcggt cttcacgatg ggtagcgggc aggacaggcc cttcagatcg agcgtcttgg
     2461 tgatggcctg gctcatgact cactccttcc ggtgggaacg ggcaggggca ggttctcggg
     2521 caaccgcccc ttgggaacga tgccccaata cagtcggttc aacatccatt tcgagacgtg
     2581 ccacaggacg ttcgggcttg gaggcgccgg cgggtgctcg tagtcgaagc gcagcaccgt
     2641 ggccttgcgg ttgccggtct cgaggaagca catcaccctg ccgccgtagt tctccttcgg
     2701 ggcggtgccg cggaccagcg aggcgatccg actggccacc acgggtgcct cgaaatgcgc
     2761 cgtcgacccg ctcttggaga tcggtaggtc cgtgccgtcc ccgatggcga agatccgctc
     2821 gtagccctgg acctgtagcg tggcccgatc cgtggggagc cagcccgagg cgtcgcccag
     2881 gttggactcg gagaccacgg tctggccgcg gtggggcggg accagcacca gcaggtcgta
     2941 ctccgccttc tctccttcca gggaggacac cacgccggcg gaggggtcca cggactcgac
     3001 gttgaagaag gtcgtcaggc cgatcccacg ctcctccatg atgggctgga tcagcttgga
     3061 cgcggcctcg atggtgaacg cccggttcaa gggcgatagc agcgtcacct cggtccgctc
     3121 gcggaggccg cgtcgtcgga ggtactcctc gagcatgaag acgaactcga tcggggccgg
     3181 cgggcatcgg taggggatgc ccgcgatccc caccaggatc cgtccccccg agaagccccg
     3241 cagggcctcc cgcagccgct gggctccatc gagcgagtag aactcgtagg atccctcctc
     3301 gagcccgggg atctggtccg gggccagacg agccccggtc gccatgacca ggtagtcgaa
     3361 ctcgatgcct cccccccggg ccagctgcac ggtccccgcg tcggggtgga tccggacggc
     3421 ctcgtccacg agcagcccca cgtccgggcg gagcaacgtg cgctcgtcgc gcgtcagcca
     3481 tcggccgttg gcctggccca gggccacgta caggtatccg ggctggtaga cgtgcatgcc
     3541 ggtggggtcg acgacggtga cggccacgtc cgcccccagc tccctggaga ccaggttcgc
     3601 agccagggtc ccgccgaccc caccgccgag cacgaccacc cgagccggca cgtcctcacc
     3661 gggccttccg aaccacgaac cgggcgaacc catcgtcctc gaccgtttcc accatctcgt
     3721 gcccggcctt gtggatccac gccgggatgt cggtcctgga tccctcgtcg ctggacagga
     3781 cctcgatcac cgctcccacc tcgctctcgc ggatggcgcc gatgagagac atcagcgggc
     3841 cgggacaggg catgccccgt gcgtcgatcg atcggtcgat gtgggtcacg cctgccatcc
     3901 ccgacacctc ctcagatgaa ggtcaccggg ccgtcgacct ccatgaagaa cgaggccacg
     3961 ccctcctggt cgtccacgac cggatccagg tcgtccatgg tcagtccgaa catctccatc
     4021 gacagcgagc aggcgtggat cgccacttcg ccgatcgact tcgcctgctg gaagatctcg
     4081 ctccagtgct gccccccgtg acgctgcagg atctgagctc ccgcgggacc ggcctcggga
     4141 gcgacgccgt ggtccttggc gatgcgatcg gcgcggaacg cgtcgagggc ccagtactgc
     4201 aggaagaggt tcaccttccg gcccatcgcg gcggctcccg ccgtcagcac ggcagcggcg
     4261 ttcagcttgt cgtctgtccc tgagaacagg accaacgaga gcgtctggcc gtccatctca
     4321 tcgcaccccg tcgcttcagt gatgccgttc cgcccctagg gtccaggacg gtggcaacgc
     4381 tcgagagggc cggagtaccc gcttcggcag ggaccgttgg gccggttgcg acgggaagcg
     4441 ggccctggct accgtcggat cgtgaca
//
LOCUS       DASQQQ010000166         2924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_38421, whole genome shotgun sequence.
ACCESSION   DASQQQ010000166 DASQQQ010000000
VERSION     DASQQQ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38421"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /gene="murA"
                     /locus_tag="VE646_06765"
     CDS             <1..971
                     /gene="murA"
                     /locus_tag="VE646_06765"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZD79725.1"
                     /translation="ARFGRARVAMPGGCNIGSRKIDLHVRGLEKMGARFLSEHGYLQA
                     SAPAGLRGAVISLDFPSVGATENVLMAAVAARGTTVIENAAREPELVDLVELLTEMGA
                     LIQGAGTPTIEVEGTDRFQAAEHTVIPDRIEAGTYAMAACATGGEVELLGARSDHLDL
                     VLAKLSELGAEVRETEHGIAVSLQGRPAPADLVTLPYPGFPTDLQPQIMALLATADGT
                     SIVTENVFESRFMFVDELNRMGADIRTEGHHAVIRGVERLSAAPVRALDIRAGAAMVI
                     AALAADGITAIEDMYHVDRGYQDFESKLRSLGAEVSRRRELSAALP"
     gene            998..2686
                     /locus_tag="VE646_06770"
     CDS             998..2686
                     /locus_tag="VE646_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79726.1"
                     /translation="MAVLVAGWLGGGSSLAWGAQQREAFVFLVRGIAFRDVQLLPGTG
                     AALMSGGEDLPADLAAGAPSGARIVDLGSLEVPGSGGSDRSLLHGAALRAAWERIGRI
                     VGASSADRILVIVACTRAPSTERLPLTPITMASGPPASVLPPGSEPVNGLPDRLRALT
                     SDSTRRIGVVSAVDIVPTIRSFLGLAPGSSGSVIRRTPDPAPFTLYERSLADRRLWFP
                     IQTAGALYVTAVGLAGIVMFLLRGRLPTRLVRGTGWLALSVIPLATALLAAGHLPSLS
                     PEIVIPFVVGVTMLSVLAVVPLHRFGTSAPTAALGAAVLVYFAVEAALGWTAALTPFL
                     GGSELDGGRFFGLPNVFIGLLIGSSLFVAARLSPWTGFALVAAVGLFAGLPSLGSNLG
                     ASIALFAAAGLWLVVRTRPRPGWQRFGTAAGVVAAGTGLVLLAHRYLTSTPTHVTRFE
                     EARGGLGAEWGRFVDRLGVGWDLIRSNPFALVPVIGVVATTALVLWPPGSVRIRLALV
                     PELAQAVTVTLVAGVVAYVANDSGPAATGLAFGLGLAGLLYVLLASRTGMMEAP"
     gene            2683..>2924
                     /locus_tag="VE646_06775"
     CDS             2683..>2924
                     /locus_tag="VE646_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79727.1"
                     /translation="MSDPVPPRPDLGPSAPPGDRASPAGRVRSTWSWWEAVGVYLLIT
                     LASSTVASPLLLIRPKGVAELVASAVIAVLNVGLLVL"
BASE COUNT          309 a         1034 c         1099 g          482 t
ORIGIN      
        1 tggctcggtt cgggcgagcc cgcgtggcca tgcccggcgg ctgcaacatc gggtcccgga
       61 agatcgatct gcacgtccgt gggctggaga agatgggtgc tcggttcctg tccgagcacg
      121 ggtacctgca ggccagcgcc cccgcggggc tccgcggtgc cgtgatctcg ctggatttcc
      181 ccagcgtcgg cgccaccgag aacgtgctga tggcggcggt ggccgcccgg ggcaccacgg
      241 tgatcgagaa cgcggctcgg gagcccgagc tggtggatct ggtggagctc ctgaccgaga
      301 tgggggcgct gatccaaggg gcgggcactc cgaccatcga ggtggagggc accgaccggt
      361 tccaggccgc ggagcacacg gtgatccccg atcggatcga ggcggggacc tacgccatgg
      421 ccgcctgcgc caccggaggg gaggtcgagc tgctcggggc tcgttccgac cacctggacc
      481 tcgtgctggc caagctgtcg gagctcgggg ccgaggtgcg tgagaccgag cacgggatcg
      541 cggtgtcgct gcaaggacgg ccggccccgg ccgatctggt gactctcccg taccccgggt
      601 tccccaccga tctgcagccc cagatcatgg cgctgctggc cacggcggac ggcaccagca
      661 tcgtgaccga gaacgtcttc gagagccggt tcatgttcgt ggacgagctc aaccgaatgg
      721 gggccgacat ccgcaccgag gggcaccacg ccgtgatccg aggggtggag cgtctgtccg
      781 ccgcccccgt gcgggccctg gacatccgag ccggagccgc catggtgatc gccgccctcg
      841 cggccgacgg catcaccgcg atcgaggaca tgtaccacgt ggaccgcggc taccaggatt
      901 tcgagtcgaa gctgcggtcc ctgggggccg aggtgagccg ccggcgggag ctctccgccg
      961 cgctcccgtg acccgcgcgc tcggtcgcct ggccgtcgtg gcggtgctcg tcgccgggtg
     1021 gctgggtggg ggctcgtccc tcgcgtgggg agcgcagcag cgcgaggcct tcgtgttcct
     1081 ggtccggggg atcgccttcc gcgacgtcca gctgctgccg ggcaccggcg cggcgctgat
     1141 gagcggaggc gaggacctgc cggccgacct ggcggcgggg gcgccgtccg gcgcgcggat
     1201 cgtcgatctg gggtccctcg aggtgccggg ctcgggtggg tccgaccgat cgctcctcca
     1261 tggtgccgcc ctccgggccg cctgggagcg gatcgggagg atcgtcggcg cctcgtccgc
     1321 ggaccggatc ctggtgatcg tcgcctgcac ccgagctccg agcaccgagc gtctcccgct
     1381 cacgcccatc accatggcct cgggaccccc ggcgagcgtg cttccgccgg ggtccgaacc
     1441 ggtgaacggc ctgcccgacc ggctccgagc gctgacctcc gactccaccc gtcggatcgg
     1501 cgtggtctcc gccgtagata tcgtgcccac gatccgttcc ttcctgggcc ttgcccccgg
     1561 gtcctccgga tccgtgatcc gccggacgcc ggacccggcc ccgttcacgc tgtacgagcg
     1621 ctccctggcc gatcgccgcc tgtggttccc gatccagacg gccggcgccc tgtacgtgac
     1681 ggccgtgggg ctggcgggga tcgtgatgtt cctgctgcgc ggtcgcctcc cgacccggct
     1741 ggtgcgcggc acgggctggt tggcgctctc ggtgatcccg ctggccacgg cgctcctcgc
     1801 cgcgggccac ctgccgagcc tctcccccga gatcgtgatc ccgttcgtgg tgggggtcac
     1861 gatgctctcg gtgctggccg tcgtgccgct gcaccggttc ggcacctcgg cacccacggc
     1921 ggccctggga gcggccgtgc tggtgtactt cgcagtggag gccgcgctgg gatggacggc
     1981 cgctctcacc cccttcctgg gtggatcgga gctggacgga ggtcggttct tcggcctgcc
     2041 gaacgtgttc atcggcctgc tgatcggctc atcgctgttc gtggccgccc ggctgtcccc
     2101 ctggaccggc ttcgcgctcg tcgcggcggt ggggctcttc gccggcctgc cgtcgctggg
     2161 ctcgaacctg ggggcctcga tcgccctgtt cgcggcggcg gggttgtggc tggtggtccg
     2221 gacccgaccc cgtcccgggt ggcagcgctt cgggacggcg gccggcgtgg tggccgcggg
     2281 caccggcctg gtgctcctgg ctcaccgcta cctgacctcg acccccaccc atgtgacccg
     2341 cttcgaggag gcgcgcggcg gcctcggcgc cgagtggggg cggttcgtgg accgcctggg
     2401 cgtgggctgg gatctgatcc ggtcgaaccc gttcgccctg gtgcccgtga tcggcgtggt
     2461 ggcgaccacg gccctggtgc tgtggccgcc cgggtcggtg cggatccgcc tggcgctcgt
     2521 ccccgagctc gcgcaggccg tgaccgtcac cctggtggcg ggcgtggtcg cgtacgtggc
     2581 caacgattcg ggccccgcgg ccacggggct tgccttcggg ctgggactgg cagggctgct
     2641 gtatgtgttg ctggcgagcc gaacagggat gatggaggca ccatgagcga tccggtcccc
     2701 ccgcgccccg acctgggacc gagcgcgccg ccgggggatc gggcgtctcc ggccgggcgg
     2761 gtccgctcca cgtggtcctg gtgggaggcc gtcggcgtct acctgctgat cacgctggcc
     2821 tcctccacgg tcgcctcccc gctgctgctg atccggccca agggggtggc cgagctggtg
     2881 gcctcggcgg tgatcgccgt gctgaacgtg ggtctgctgg tgct
//
LOCUS       DASQQQ010000167         4753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_262058, whole genome shotgun sequence.
ACCESSION   DASQQQ010000167 DASQQQ010000000
VERSION     DASQQQ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262058"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="VE646_06780"
     CDS             complement(<1..1147)
                     /locus_tag="VE646_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZD79728.1"
                     /translation="MSTRLEERTRGGVGQSVRRVDGIPKVEGAFLYGSDLWHEQMLWG
                     ATLRSPHPHARIRSVGIWEAVASPGVHAVLTHEDLPGARTYGLEFSDQPVLAWDRVLY
                     AGQPVAVVAAETPELARRAAQRIRVDYEVLPPVLDMTEALDPSAPRLHEWGNVLRHVR
                     IEHGDPDAGAEVWVEGYYETGMQDQAALGPEAGLAIPAEDGGIDLIVSTQWLHVDRRQ
                     IAPCLNLPEDKVRITLAGVGGAFGSREDLHMQIHACLLALHTGRPVKMSYGRPESFLA
                     HVHRHPSRVWMRTGAARDGTLIAVRARVVIDGGAFASSSSAVIGNATTFLPGPYEVPN
                     ALLEGTAVYTNNPPCGAMRGFGAVQVCFAHEGQMDRLAAALGMDAVEL"
     gene            complement(1147..2523)
                     /locus_tag="VE646_06785"
     CDS             complement(1147..2523)
                     /locus_tag="VE646_06785"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HZD79729.1"
                     /translation="MSRLLIERCEVVAVMDDAGTELAGGSILIEDGRISWVGSGAPPG
                     AEGVERIDGRGCVAAPGLVNTHHHLFQTLTRARAQDKGLFDWLVELYPVWASVDEEWV
                     GIAARVGLAELALSGCSTTTDHHYIFPAGAGDLMAAEIDAARQIGLRFHPARGSMDRG
                     ESGGGLPPDATVEDTDSALEATEDAVRRFHDPSPGSMLRIAVAPCSPFSVSERLMRGS
                     AELARRLRVRLHTHVAETLDEERHCLERFGGRPLELLEEWGWLGEDVWLAHCVHLDGD
                     DVKRMGVTSTGAAWCPSSNLRLASGIAPARALLDAGAPVGLGVDGSASNDAGDLLAEA
                     RQGLLVARAGGDPTAMSARQVLRAATRGGAACLGREDELGSLGPGKRADVALFDLTGL
                     GHAGAQADPVAALVFTSPGGVRDLLVEGRPVVRDGHLVRADEEAIAAQGRRRARSISG
                     GAGPGGGG"
     gene            complement(2520..2993)
                     /locus_tag="VE646_06790"
     CDS             complement(2520..2993)
                     /locus_tag="VE646_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZD79730.1"
                     /translation="MLVRANVNGEWREAHVWPGESLLFLLREELGLPGSKNACEQGEC
                     GSCSVWLDGELVCACLVLAGQAHQHQVRTVEGLAGGQTLHPVQEAFLEAGAVQCGFCT
                     PGLIVAVTDLLEHDPSPDELEVREALSGNLCRCTGYEKILRAVSLAAERKREGAW"
     gene            complement(3006..3884)
                     /locus_tag="VE646_06795"
     CDS             complement(3006..3884)
                     /locus_tag="VE646_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HZD79731.1"
                     /translation="MDVLLPRTLEEALEIRAERPHAVPIAGGTDLMVAMNLEGYRPEA
                     ILDVSRLPELRGWRREDDTVFLGAGVTFARIQRELQDLRALVQASRSVGSPQIRNRGT
                     VGGNLGTASPAGDALPVLGVHDAEIELAAAGGRRRRLPWNAFLQGVKRTALTPDELIT
                     GATWRALTGPQSFSKIGTRNAMVISVVSLCLALDPATRTVRVALGSVAPTVVRAAEAE
                     TFAAAAMERAGSWDEPAPLGGPDLEGFAERVVAAVAPIDDVRGTAAYRRHAVAVLARR
                     ALGWAIDERAEEAMVG"
     gene            complement(3884..>4753)
                     /locus_tag="VE646_06800"
     CDS             complement(3884..>4753)
                     /locus_tag="VE646_06800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZD79732.1"
                     /translation="VPMAAFAATTDRIKVGSGVVNIWTRNAGLMGATFVTLDDLAPGR
                     VMLGIGAWWEPLASKVGVDRRRPLQAMREYVEVTRRLIAMERVTYHGAFVHVEDIQID
                     VVHGDPSPRNVPIYIGATGMRMMELAGEIADGVLLNYLVSPGYDHTAMAHLAVGAERG
                     RRSVEDLDRPQLVVCSLDEDRGRALDRARELVTQYLGQQPHIMKASGVDQGLLDEIGK
                     VLTWPAGPEEIRRAKALVPDEVVQMITASGTADECRAKVREYVEAGCTCPILYPLGDD
                     VRATIDAFADGVA"
BASE COUNT          639 a         1867 c         1637 g          610 t
ORIGIN      
        1 gcagctcgac cgcatccatc ccgagggcgg cagcgagccg gtccatctga ccttcgtgcg
       61 cgaagcacac ctgcaccgcg ccgaaccccc gcatcgcgcc gcacggcggg ttgttcgtgt
      121 agacggccgt cccctcgagc agcgcgttcg gcacctcgta ggggccgggc aggaaggtcg
      181 tggcgttgcc gatcaccgcc gatgaggacg aggcgaacgc gccgccgtcg atcaccaccc
      241 gagcccggac cgcgatcagc gtgccgtcgc gcgcggcccc ggtccgcatc cacacgcgcg
      301 aggggtggcg gtgcacgtgg gccaggaagg actcgggccg accgtaggac atcttcaccg
      361 gccggccggt gtgcagggcg agcaggcagg cgtggatctg catgtggagg tcctcgcgcg
      421 agccgaaggc gccccccaca ccggccagcg tgatccggac cttgtcctct ggcaggttca
      481 ggcagggagc gatctgccga cggtccacgt ggagccactg cgtcgagacg atcaggtcga
      541 tgcccccgtc ctcggcgggg atggccaggc cggcctcggg tccgagggcc gcctggtcct
      601 gcatcccggt ctcgtagtag ccctcgaccc acacctcggc cccggcgtcg ggatcgccgt
      661 gctcgatgcg cacgtgccgc agcacgttgc cccactcgtg cagccgcggc gccgacggat
      721 cgagcgcctc ggtcatgtcc aggaccgggg ggaggacctc gtagtcgacc cggatccgtt
      781 gggccgcccg ccgggccagc tccggggtct ccgccgccac gacggcgacc ggctggcccg
      841 cgtacaggac ccggtcccat gcgagcacgg gctgatcgga gaactccagg ccgtaggtcc
      901 tcgctccggg gaggtcctcg tgcgtcagga cggcgtgcac gccggggctg gccaccgcct
      961 cccagatgcc caccgagcgg atccgcgcgt gggggtgcgg gctccgcagc gtggcgcccc
     1021 acagcatctg ctcgtgccac aggtcgctgc cgtacaggaa ggcgccctcc accttgggga
     1081 tgccgtccac ccgtcggacg ctctggccga ccccgccccg ggtccgctcc tcgagccggg
     1141 tgctcatcac ccaccgcctc ccggaccggc cccgcccgag atcgaccggg cccggcggcg
     1201 tccctgggcc gcgatcgcct cctcgtcggc cctgaccagg tggccgtcgc gaaccaccgg
     1261 acgcccctcg accagcaggt cccggacccc gccgggggag gtgaagacca gagccgccac
     1321 ggggtcggcc tgcgcaccgg cgtgcccgag gccggtgagg tcgaacagcg ccacgtccgc
     1381 ccgcttgccc ggcccgagcg agcccagctc gtcctcccgc cccaggcacg ccgcgccgcc
     1441 gcgcgtcgcg gcccgcagca cctgccgggc gctcatggcc gttgggtcgc cgcccgcgcg
     1501 ggccaccagc aacccctgcc gggcctcggc caacaggtcc cccgcgtcgt tcgatgccga
     1561 cccgtccacg cccaggccca ccggcgcacc ggcgtccagc agggcgcggg cgggagcgat
     1621 gcccgaggcc agccgcaggt tcgaggaagg gcaccaggcc gcgccggtgg aggtgacccc
     1681 catgcgcttg acgtcgtccc cgtccaggtg gacgcagtgc gccagccaga cgtcctcgcc
     1741 cagccagccc cactcctcga gcagctcgag cggccggccg ccgaaccgct ccaggcagtg
     1801 gcgctcctcg tccagggtct cggcgacgtg ggtgtgcagg cggacccgga gccggcgggc
     1861 cagctccgcc gagccgcgca tcagccgctc gctcaccgag aacggcgagc agggcgccac
     1921 ggcgatccgc agcatcgatc ccggcgacgg gtcgtggaac cggcggacgg cgtcctcggt
     1981 ggcttcgagc gcggagtccg tgtcctccac ggtggcgtcg gggggcaggc cgccaccgga
     2041 ctccccccgg tccatcgagc cgcgcgcggg gtggaagcgc agaccgatct gccgggccgc
     2101 gtcgatctcg gcggccatca ggtcgccggc gccggccggg aagatgtagt ggtggtcggt
     2161 ggtggtgctg cacccggaca gggccagctc ggccaggccg acgcgcgccg cgatccccac
     2221 ccactcctcg tccaccgatg cccacaccgg gtagagctcc acgagccagt cgaacagacc
     2281 cttgtcctgg gcccgcgccc gggtgagggt ctggaacagg tggtggtgcg tgttcaccag
     2341 cccgggcgcc gccacgcatc cccgcccgtc gatccgttcg acgccctcgg ccccgggcgg
     2401 cgcccccgag ccgacccacg agatgcggcc gtcctcgatc aggatcgagc cgccggcgag
     2461 ctcggtgccg gcgtcgtcca tgaccgccac cacctcacag cgctcgatca gcagccggct
     2521 caccaggccc cctcccgctt gcgctcggcg gcgaggctca cggcccgcag gatcttctcg
     2581 tagccggtgc accggcacag gttgccggac agtgcctcgc ggacctccag ctcgtccggc
     2641 gaggggtcgt gctccagcag gtcggtcacc gcaacgatca gccccggcgt gcagaacccg
     2701 cactgcacgg cgccggcctc caggaacgcc tcctgcaccg ggtgcagggt ctgccccccc
     2761 gccaacccct ccacggtcct gacctggtgc tggtgggcct gcccggcgag caccaggcac
     2821 gcgcagacca gctcgccgtc cagccacacg gagcacgagc cgcactcccc ctgctcgcag
     2881 gcgttcttcg agccggggag gccgagctcc tcgcgcagca ggaacaggag gctctcgccc
     2941 ggccagacgt gggcctcccg ccactcgccg ttcacgttcg ctcggacgag cacctcaggc
     3001 gtccctcatc cgaccatcgc ctcctcggcc cgctcatcga tcgcccagcc gagggcacgg
     3061 cgggccagga ccgcgacggc gtgccgccgg tacgcagccg tcccccgcac gtcgtcgatg
     3121 ggtgccacgg ccgccacgac ccgctcggcg aagccctcga ggtccggtcc gccgagcggc
     3181 gccggctcgt cccaggagcc ggcgcgctcc atcgcggcgg ccgcgaaggt ctcggcctcg
     3241 gcggcccgta ccaccgtggg tgcgaccgag ccgagggcca cccggacggt ccgggtggcc
     3301 gggtccaggg ccaggcacag gctcaccacc gagatcacca tcgcgttgcg ggtcccgatc
     3361 ttggagaagg actgcggtcc ggtcagggcg cgccacgtcg cccccgtgat cagctcgtcg
     3421 ggcgtcaggg cggtgcgctt cacgccttgg aggaacgcgt tccagggcaa gcgtcgtcgg
     3481 cggcccccgg cggcggccag ctcgatctcc gcgtcgtgga cgcccaggac gggaagcgca
     3541 tcgcccgccg gggaggccgt ccccaggttg ccgcccacgg tcccgcggtt gcggatctgc
     3601 ggcgacccca ccgagcgcga ggcctgcacc agggcccgga ggtcctgcag ctcgcgctgg
     3661 atccgagcga acgtcacccc cgcgcccagg aagacggtgt cgtcctcgcg tcgccaccct
     3721 cgcagctcgg gcaggcggga cacgtccagg atcgcctccg gccggtatcc ctcgaggttc
     3781 atcgccacca tgaggtcggt gccgccggcg atcgggacgg cgtgcggccg ctcggccctg
     3841 atctccagcg cctcctccag cgtgcgcggc aggaggacgt ccatcaggcg accccgtccg
     3901 cgaaggcgtc gatcgtggcg cgcacgtcgt cccccagcgg gtacaagatc gggcacgtgc
     3961 atcccgcctc cacgtactcg cggaccttgg cccggcactc gtcggccgtg cccgaggccg
     4021 tgatcatctg caccacctcg tcgggcacca gggccttcgc ccggcggatc tcctcgggcc
     4081 cggcgggcca ggtgagcacc ttcccgatct cgtccagcag cccctgatcc acgccgctgg
     4141 ccttcatgat gtggggctgc tggcccaggt actgcgtcac cagctctcgg gcccggtcca
     4201 gggcgcggcc ccggtcctca tccagcgagc acaccacgag ctggggccga tcgagatcct
     4261 ccaccgaccg ccggccacgt tcggcgccga ccgccagatg ggccatcgcg gtgtggtcgt
     4321 agccggggct caccaggtag ttgagcagca cgccgtcggc gatctcgccc gccagctcca
     4381 tcatccgcat gcctgtggcg ccgatgtaga tcggcacgtt ccggggcgag ggatccccgt
     4441 ggacgacgtc gatctggatg tcctccacgt gtacgaacgc accgtggtag gtgacccgct
     4501 ccatcgcgat cagccgccgg gtgacctcca cgtactcgcg catcgcctgc agcggccggc
     4561 ggcggtccac ccccaccttc gaggccagcg gctcccacca ggcgccgatc ccgagcatga
     4621 cccgtccggg agcgaggtcg tcgagcgtga cgaacgtggc ccccatgagc cccgcgttgc
     4681 gggtccagat gttcaccacc cccgagccca ccttgatgcg gtcggtggtc gcggcgaaag
     4741 cggccatggg aac
//
LOCUS       DASQQQ010000168         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_69600, whole genome shotgun sequence.
ACCESSION   DASQQQ010000168 DASQQQ010000000
VERSION     DASQQQ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69600"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..922)
                     /locus_tag="VE646_06805"
     CDS             complement(107..922)
                     /locus_tag="VE646_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007084534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZD79733.1"
                     /translation="MLTDPSLPAAGRPLPRVLAVANQKGGVGKTTTAVNLGAALAELD
                     YRVLVVDLDPQGNATTGLGLNPRNLETSMYHVVMSDVAIEDCIEPTSVRNLFVAPASL
                     DLAGAEIELVPAFSRELRLRRALEEVTDQYDFVLIDCPPSLGLLTVNGLAAAGEVLVP
                     IQCEYYALEGLGQLLRNINLVQKNLNPRLELTTIILVMYDARTKLSDQVVHEVQEHFG
                     DRVCRNVVPRTVRLSEAPSFGQPIIAFDSSSRGAIAYRELAKEVSGGTPRRTG"
     gene            complement(1347..>2005)
                     /locus_tag="VE646_06810"
     CDS             complement(1347..>2005)
                     /locus_tag="VE646_06810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HZD79734.1"
                     /translation="EPGRLTTPFGPREGLVRVLERAHRLGFIGDPPLAAHLDHAQGYA
                     AAWDTEGGVPAGPGMAADLGSGGGLPALPLVLHWPASRWVLIEAGDRRAAFLESAVRD
                     LDLGDRVVVRHERAEDAGRDPDLRAGCDLVTARSFGGPPVTAECAAPLLRVGGLLLVS
                     EPPVAEPDRWPTAGLAELGFRRGASIDGPPALQVLHLESACPPRYPRRVGVPGKRPLW
                     "
BASE COUNT          280 a          696 c          742 g          287 t
ORIGIN      
        1 gccccggggc tgatggggat tggggacgat gctcgtggtg gccaggtcct gaagcatggc
       61 gtccggtgtg ccggacgcct ccggcgggat cagcgctccc aggcccttac ccagtccgcc
      121 gcggcgtgcc accgctcacc tccttggcca gctcgcggta ggcgatggcg ccccgcgagg
      181 acgagtcgaa cgcaatgatc ggctggccga acgacggcgc ctcggacagc cgtacggtgc
      241 gggggaccac gttccgacac acccgatcgc cgaagtgttc ctggacctcg tgcacgacct
      301 ggtcggaaag cttggtgcgg gcgtcgtaca tgacgaggat gatcgtcgtc agctccagcc
      361 ggggattgag gttcttctgc acgaggttga tgttgcggag gagttgcccc aggccctcca
      421 gggcgtagta ctcgcactgg atcggcacca ggacctcgcc ggcggcggcc aggccgttga
      481 ccgtgagcag ccccaacgac ggtgggcagt cgatgagcac gaagtcgtac tggtcggtaa
      541 cttcctccag cgctcgtcgc aggcgcagct cccggctgaa ggcgggcacc agctcgatct
      601 ccgcgccggc gaggtcgagg ctggccggcg ccacgaacag gttccgcacc gaggtgggct
      661 cgatgcaatc ctcgatggcc acgtcgctca tgaccacgtg gtacatcgag gtctccaggt
      721 tgcgggggtt cagtcccagg ccggtcgtgg cgttgccctg aggatccagg tccacgacca
      781 gcacccggta gtcgagctcg gccagggcgg cgccgaggtt caccgcggtg gtcgtcttcc
      841 ccacgccgcc cttctggttg gccaccgcca ggacacgggg gagtggacga ccggcggcgg
      901 ggagcgacgg atcggtgagc agctgttcgg tctgctcgag gatcggctcc tgcaaccgcg
      961 gttcctcagc gaccggttcc gcggcttcgg gtggcggcgc ggcttcgctg gcgggggcct
     1021 cggtggcggg ggcctcggtg gcgggggcct cgcccggggg agcgcccgac ggcccatcga
     1081 tgacgtcgga cgccgccacc ggggcgtgct cctggtctga tgccgtctcc tgaggagccg
     1141 cttcctcgtc gtccgcccgc gcccgcagcc gttggaagat catccggccg cgcgcgaccg
     1201 gatccgaggg gctgtcggtc atcccggcga tgttgcccgc ggcatcccac gccggtcaag
     1261 gactcattga ggcttctcgt cgactttccg ggcgggagcg tgacctcccc cgcgtgtttc
     1321 ccgtgaaaca cggcgaggtt cggtgctcac cagagcgggc gcttcccggg cacaccgacc
     1381 cggcggggat agcggggcgg gcaggccgac tccaggtgga ggacctgcag tgcggggggc
     1441 ccgtcgatgg acgctccccg ccggaacccc agctcagcca acccggccgt cggccagcgg
     1501 tcgggctcag cgacgggagg ttcgctcacg agcaggaggc cgccgacccg gagcaggggc
     1561 gcggcgcact cggccgtgac cggcgggcca ccgaaggaac gggcggtcac caggtcgcaa
     1621 ccggcccgga ggtcggggtc ccggccggcg tcctccgccc gctcgtggcg caccacgacc
     1681 cgatcgccca gatcgaggtc gcggacggcg ctctccagaa aggcggcgcg gcggtcaccc
     1741 gcttcgatca gcacccagcg gctggcaggc cagtgcaaca ccaacggcag ggccggcaat
     1801 cccccgccac tccccaggtc ggcggccatt ccggggccgg ccggcacacc accctcggtg
     1861 tcccaggcgg cggcgtagcc ctgggcgtga tcgaggtggg cggcgagtgg cgggtcgccg
     1921 atgaacccca ggcggtgagc ccgctccagc acacggacga ggccctcgcg cgggccgaaa
     1981 ggggtggtca gtcgtccggg ctctc
//
LOCUS       DASQQQ010000169         3897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_397133, whole genome shotgun sequence.
ACCESSION   DASQQQ010000169 DASQQQ010000000
VERSION     DASQQQ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397133"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VE646_06815"
     CDS             <1..958
                     /locus_tag="VE646_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZD79735.1"
                     /translation="EGAEARALSCGFPATTATSLASVGTGVPPGEHGVVGYTFAMPDL
                     DRPMNALRWEPYGPGKRTDLRSTYPPELVQPTSTLFERASACGVETVMVGGVEHVASG
                     LTRAVFRGAGRRPGFGMGDVATTGAALLRDRTARFVYAYHADLDTTAHVRGVGSESWA
                     VHLSYVDRLAADLAERLPAGTVLAITGDHGTVDVDPADRIDLGHHPELLEGVAMVGGE
                     ARARHVYAAPGAGADVLAAWTETLGPRAWVRSREEAIQAGWFGPVVGPEARSRIGDVV
                     AAARAAVAVVQRELDGVQASLIGHHGSFDPAEQLVPFLIHRT"
     gene            complement(985..>3897)
                     /locus_tag="VE646_06820"
     CDS             complement(985..>3897)
                     /locus_tag="VE646_06820"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HZD79736.1"
                     /translation="PIILTRAPGYVLLVDAGSIDARRFERLITHGVHELGAGDRSSGT
                     ADLRAALELWRGPPLSDFAYEEFAQPDIHRLQELRAEAIEELAEAEIDAGNTMGILPL
                     LDAAIREDPLRERARELQMLALYRAGRQAEALRTFQRYRLMLAEELGLDPSPGLRRLQ
                     ERILVHDPTLVPQRAAEPGVTRNPFKGLRAFEEGDREDFFGRDVLVHQLVERFAAGAR
                     LIAVVGPSGCGKSSVVNAGLIPAVRSGELPGGWRIERILPGRHPTGELQRVQEGSEPI
                     LLVLDQFEQLFTEADPAERDAFLDRLATLAANPRGPWRIVLTLRADFYDRPLLHPAFA
                     GIFTAGVVNVMPMSSDELESAIAGPAERAGLEVQPALLAELVAQTLGRTGALPLLQYV
                     LTELFDRRRDSALTLDAYRASGGLQGTLTHRAEELHASLNDQQRRIAKQVFLRLTRLG
                     EGTRHARRRTRIGELTSLELDPVALSEVLERFGSYRLVSFDHDLLTGDATVEIAHEAL
                     LWEWDRLAGWIEEHRIDLRKHLALVAAIEEWQASGRQPDYLLSGSRLAEYQTWASRTT
                     LRLTEEEGSFLDAGLERRLSLQAEEAARAQQQRHLERRARTRLIALVTAVIVLIGGAT
                     YALFGRLTDRPPDVVLVWEGMHGDGGWADMIGKGFDAGVARLRMDAERRIVPLTAPER
                     FAPELRAAAGEGARLVVIGGTFEDAEGTAAVARDHPDTHFVSVDWVGPTVPNITYVRF
                     REEEGSFLAGAAAALKSRTGRIGFIGGAGVWTIWRCEAGYEAGARWVDPEVRVRSVYL
                     QSDTPEGGLADDDQAWRKATRMYQRGADVIFAAAGGSGFGVFKAAALTSEDVGRHLWA
                     IGVDSDQYRTVVPALRDQVGEEIAARLQRHILTSMVKHLDTAVFTTMSRYANGALRPG
                     VRRLGLAEGGVDIAYSGGFIDDVRPELEHLRRRIIAGEITVPVVPSSMGKIPGAGT"
BASE COUNT          569 a         1375 c         1380 g          573 t
ORIGIN      
        1 ggagggtgca gaggcccgag ccctgtcctg tgggttcccg gccaccaccg ccaccagcct
       61 ggcctcggtg gggacggggg tcccgcccgg cgagcacggc gtggtgggct acacgttcgc
      121 catgcccgac ctggatcgac cgatgaacgc gctccggtgg gagccgtacg ggccggggaa
      181 gcggaccgat ctgcgctcca cctaccctcc cgagctggtc caacccacct ccacgctgtt
      241 cgagcgcgcc tccgcctgcg gcgtggagac cgtgatggtg ggcggggtcg agcatgtggc
      301 gagcgggctc acccgagccg tcttccgggg ggcgggcagg cggcctggct tcgggatggg
      361 ggacgtggcg accacggggg ccgcgctgct ccgcgaccgg acggcccgct tcgtgtacgc
      421 ctaccacgcg gacctggaca ccaccgccca cgtccggggc gtgggatcgg aatcctgggc
      481 ggtccatctc tcgtacgtcg accggctggc ggcggacctg gccgagcgcc tgccggcggg
      541 gacggtgctg gcgatcaccg gggaccacgg cacggtcgat gtcgatcccg ccgaccggat
      601 cgacctgggg catcaccccg agctgctcga gggcgtggcg atggtgggcg gcgaggctcg
      661 agcccggcac gtgtacgccg ctccgggcgc cggcgccgat gtcctggccg cctggaccga
      721 gacgctgggg ccgcgcgcat gggtccgctc gcgtgaggag gccatccagg cggggtggtt
      781 cggcccggtg gtcggcccgg aggctcgatc acggatcggg gacgtggtcg ccgcggcgcg
      841 tgcggcggtg gcggtggtgc agcgcgagct ggatggcgtc caggcctcgc tgatcggcca
      901 tcacggctcg ttcgatccgg ccgagcagct ggtgccgttc ctgatccatc ggacgtgagc
      961 gtgcgctggt ggtgactggg agcttcaggt tccggctcca gggatcttgc ccatactgga
     1021 cggaaccacg ggcacggtga tctcgccggc gatgatccgt cggcgaaggt gctcgagctc
     1081 cggacggacg tcgtcgatga acccaccgct gtaggcgatg tcgacgccac cttcggccag
     1141 gccgagccgt cgcacgccgg gacgaagggc cccgttcgcg taccgcgaca tggtcgtgaa
     1201 gaccgcggtg tcgaggtgct tcaccatgga ggtcaggatg tgccgctgca accttgccgc
     1261 gatctcctcg cccacctggt cgcgcagcgc ggggaccacc gtgcggtatt gatcggagtc
     1321 cacgccgatc gcccacaggt gtcggccgac gtcctcggac gtgagggctg ccgccttgaa
     1381 cacgccgaac cccgacccac ccgcggccgc gaagatcacg tccgcgcccc gctggtacat
     1441 cctcgtcgcc ttgcgccatg cctgatcgtc atcggccaac ccgccctcgg gggtgtccga
     1501 ctggaggtaa acggacctga cccgcacctc gggatcgacc catcgagcgc cggcctcgta
     1561 gccggcttcg catctccaga tcgtccacac gcccgccccg ccgatgaaac cgatcctccc
     1621 ggtcctgctc ttgagcgccg ccgccgcccc cgcaaggaac gaaccctcct cttctcggaa
     1681 gcgcacgtac gtgatgttgg ggaccgtcgg gcccacccag tccacggaca cgaagtgggt
     1741 gtcggggtgg tcccgagcaa ccgccgcggt tccctcggcg tcctcaaagg tccccccgat
     1801 caccaccaga cgggcgccct cgccggcggc cgcccggagc tcaggagcga accgctcggg
     1861 cgcggtgagg ggcacgatcc tccgctccgc gtccatgcgg agccgagcaa cgccggcatc
     1921 gaagcccttg ccgatcatgt cggcccagcc accatccccg tgcatcccct cccacaccag
     1981 caccacgtcc ggcgggcgat cggtcaaccg accaaagagc gcgtaggtgg ccccgccgat
     2041 cagaacgatc accgccgtca ccagggcgat gagtcgagtc ctggcgcgcc gctcgaggtg
     2101 tcgttgctgc tgcgcgcgcg ccgcctcctc ggcctgtagg gacagtcggc gttccaggcc
     2161 ggcgtcgagg aaggagccct cctcctcggt gagccggaga gtggtgcggc tcgcccacgt
     2221 ctggtactcg gccaggcgtg aacccgacag gaggtagtcg ggttgccggc cggatgcctg
     2281 ccactcctcg atcgcggcca cgagtgcgag gtgcttgcgc aggtcgatcc ggtgctcctc
     2341 gatccatccg gcgagccggt cccattccca gagcagcgcc tcgtgggcga tctccaccgt
     2401 cgcgtcgccg gtgagcaggt cgtggtcgaa ggacaccagt cggtagctcc cgaaccgctc
     2461 caggacctcg gacagggcga ccggatcaag ctccaaggag gtgagctcgc cgatgcgggt
     2521 gcgccgtcgg gcgtgtcggg tgccctcacc caggcgggtc agccgcagga agacctgctt
     2581 ggcgatcctc cgctgctggt cgttgaggga cgcatggagc tcctcagcgc ggtgagtcaa
     2641 ggtgccctga aggcccccgg aagctcggta ggcgtccaag gtgagtgccg agtccctcct
     2701 gcgatcgaac agctcggtga gcacgtactg cagcaaaggc agcgcgccgg tccgacccag
     2761 ggtctgggcg acgagctcgg ccaggagcgc cggctggacc tcgagacccg cccgctcggc
     2821 gggtcccgcg atcgccgact ccagctcgtc cgagctcatg ggcatcacgt tgacgacacc
     2881 ggcggtgaag atgccggcga acgcaggatg caagagcggc cgatcgtaga agtcggctcg
     2941 gagggtgagg acgattcgcc acggacctcg ggggttggcg gccagggtgg ccagccgatc
     3001 caggaacgca tcccgctccg ccgggtccgc ttccgtgaag agctgctcga actggtcgag
     3061 caccagcagg atcggttcgc taccctcctg gacccgttgg agctcgccgg tcgggtgacg
     3121 tcccgggagg atgcgctcga tcctccagcc acccggcagc tctccggagc ggacggcagg
     3181 gatcaggccc gcgttcacca cactggactt gccgcacccg gagggtccca ccacggcgat
     3241 cagtcgagct ccagcggcaa atcgctccac cagctggtgg acgaggacat cccggccgaa
     3301 gaagtcttcc cggtccccct cttcgaaggc gcggaggccc ttgaacgggt tccgcgtgac
     3361 gcccggctcc gccgccctct gtgggacgag ggtggggtcg tggacgagga tccgttcctg
     3421 caggcgccgc agccccggag aggggtcgag gccgagctcc tcggccagca tgagccgata
     3481 ccgctggaag gtgcggagcg cctcggcctg acgtcccgct cggtacagcg cgagcatctg
     3541 caactcacgg gcgcgctccc ggagggggtc ctcccggatg gccgcgtcga gcaggggcag
     3601 gatgcccatc gtgttcccgg cgtcgatctc ggcctcggcg agctcctcga tggcctccgc
     3661 tcggagctcc tgcagccgat ggatgtcggg ttgcgcgaac tcctcgtacg cgaagtccga
     3721 gagcggcggt ccccgccaga gctccagcgc cgcccggagg tccgcggtcc cggacgatcg
     3781 atctccggcc ccgagctcgt gcacgccgtg ggtgatcagc cgctcgaagc ggcgggcatc
     3841 gatcgacccc gcatccacca ggaggacgta gcccggggca cgggtgagta tgatcgg
//
LOCUS       DASQQQ010000170         3163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_151492, whole genome shotgun sequence.
ACCESSION   DASQQQ010000170 DASQQQ010000000
VERSION     DASQQQ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151492"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VE646_06825"
     CDS             complement(<1..655)
                     /locus_tag="VE646_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZD79737.1"
                     /translation="MCHEPTARPPFPPISGGAGIADRQDQVLEASDGNRFMAFTARTT
                     EPDAPGIVILPDVRGLHPFYRDLAERFAQAGVHATALDYFGRTAGTGERGEDFDHSPH
                     VERTHPDTVALDVGATVAHVRSEASGGTERVFTVGFCFGGRHSFNQAARGHGLAGVIG
                     FYGPPHRRDDEGDAPIDLAPRYRCPVLGLWGTADRATPVEQVHAFRQALDHAGVPNEI
                     "
     gene            complement(768..974)
                     /locus_tag="VE646_06830"
     CDS             complement(768..974)
                     /locus_tag="VE646_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79738.1"
                     /translation="MALTWMMIGVLTLAGIALIGYVVWRAWAPDPLGSWRRDEYDWTV
                     IRFDGAGRTRVQTVRTPSRRPGLR"
     gene            complement(1059..2114)
                     /locus_tag="VE646_06835"
     CDS             complement(1059..2114)
                     /locus_tag="VE646_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HZD79739.1"
                     /translation="MPRIRRVQAGQPGLHQRRRGRGFCYEDAGGHPVSDPQTLERIRG
                     LTIPPAWSDVWICADPWGHIQATGTDAAGRRQYLYHERWRRWRDRRKFRRMLAFGRAL
                     PGLRDVVEEHLEARDLSRERVLACAVRLLDLGGFRVGGEIYAERNGSFGLATLRKEHV
                     RVRGEAISFDFTAKGGKRRVLVVSDPGAAAVLRSLKARRDGGGDLLAHREGRDWRDVR
                     SADINAYLKEVAGDGFTAKDFRTWNATVLAAVAVAARSEVGPSLRARKRAAAAAMKQV
                     AEHLGNTPSVCRASYVDPRVIDRFLEGEVAEIPRIALDDDGVLELDDRSLREAVEAAV
                     LRLLAGSVVPVSRRPAA"
     gene            2275..>3163
                     /locus_tag="VE646_06840"
     CDS             2275..>3163
                     /locus_tag="VE646_06840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZD79740.1"
                     /translation="MATPIVARRWVSDRGSEEEGVIRFGLQIPNFTYPGVDDADLFER
                     IAQIATTAESAGFDSVWVMDHLYQIPSVGPRTDPMLEAYTLLGAIAARTSRVQLGTMV
                     TGVTYRNPALLAKIVTTLDAVSSGRAILGIGAAWNEDEHRGYGFRFPPAGERLDQLEE
                     ALRICRAMFTQQAPSFQGEHYRIHEALNVPRPIRPNGIPILVGGSGEKRTLRLVAQYA
                     DACNLFGDLDTIRHKLEVLDAHCEAVGRDPAEITKTRLGGLVIAPSKEEAERTGAEAA
                     ARRGTDLERYRAYAMIGDPD"
BASE COUNT          477 a         1174 c         1075 g          437 t
ORIGIN      
        1 cgatctcgtt cggtacgccc gcgtggtcca gtgcctgccg gaaggcgtgc acctgctcca
       61 cgggggtggc ccggtccgcg gtaccccaca gccccaggac gggacagcgg tagcgcggcg
      121 cgaggtcgat cggcgcgtcg ccctcgtcgt cgcggcggtg cgggggaccg tagaagccga
      181 tcacgccggc cagcccgtga ccgcgcgccg cctggttgaa ggagtgtcgt ccgccgaagc
      241 agaagcccac cgtgaacacc cgctcggtgc ctccactcgc ttccgagcgc acgtgggcga
      301 ccgtcgctcc cacatccagg gccaccgtgt ccggatgggt tcgctccacg tgcggggagt
      361 gatcgaagtc ctcaccgcgc tccccggtcc cggccgtacg gccgaagtaa tccagggcgg
      421 tggcatgcac accggcctgc gcgaagcgct cggccaggtc ccggtagaag ggatgcaggc
      481 cccggacgtc tggcaggatc acgatccccg gggcgtcggg ctcggtcgtg cgggccgtga
      541 aggccatgaa ccggttcccg tccgaagcct ccaggacctg gtcctggcgg tccgcgatcc
      601 cggcgccccc agagatgggc gggaacggcg gtcgggcggt gggctcatgg cacatgcatc
      661 gctcctttcc ttccgcacat gcatcgacgg gtatccctca ggacttgcgg ggcggtgcat
      721 ccgatgctat cgcgcctgcc gcgacgcgag cgtccccccc gttccggcta ccggaggccg
      781 gggcgtcgcg acggcgtccg aaccgtctgc acccgtgtcc ggccggcgcc gtcgaagcgg
      841 atgaccgtcc agtcgtactc gtcccgccgc caggacccga gaggatcagg tgcccacgcg
      901 cgccacacca cgtagccgat cagggcgatc cccgccagcg tcagcacccc gatcatcatc
      961 cacgtcaacg ccatcacgtc ctcgctccac aggcagccgt tctctacccg ggccgcgggc
     1021 agcacacacc cgaaggcaga cgaccagcgg tcctcgggtc aggccgccgg ccgtcgcgag
     1081 acgggcacta ccgatccggc caagagacga aggaccgccg cctccaccgc ctcccggagc
     1141 gatcggtcgt cgagctcgag gaccccgtcg tcgtccagcg cgatccgtgg gatctcggcc
     1201 acctccccct cgaggaagcg gtcgatcacc cgcggatcga cgtaggaggc ccgacagacc
     1261 gacggggtgt tcccgaggtg ctcggccacc tgcttcatgg ccgcggccgc ggcccgcttg
     1321 cgggctcgga gggaggggcc gacctcgctc cgcgccgcca cggccacggc ggccagcacg
     1381 gtcgcgttcc aggtccggaa gtccttggcg gtgaacccgt ctccggcgac ctccttgagg
     1441 taggcgttga tgtccgcgga ccggacgtca cgccagtcac gcccctcccg atgagccagc
     1501 aggtcgcccc cgccgtcgcg acgcgccttc agcgagcgga gcacggcggc ggcacccgga
     1561 tcgctgacca ctaggaccct gcgcttgcct cccttggccg tgaagtcgaa gctgatcgcc
     1621 tcccccctca cccgaacgtg ctccttgcgt agcgtggcca acccgaacga gccgttccgc
     1681 tccgcgtaga tctccccgcc cacccggaac ccgcccaggt ccagcaatcg gaccgcacag
     1741 gccaggaccc gctcgcgcga caggtccctg gcctccaggt gctcctctac gacgtcccgc
     1801 aggccgggaa gggcacggcc gaacgcgagc atccgccgga acttgcgccg gtcgcgccat
     1861 cgccgccatc gctcgtggta gaggtactgg cgccggcccg cggcgtccgt tccagtggcc
     1921 tggatatggc cccacgggtc ggcgcagatc cagacgtcgg accaggcagg cgggatggtc
     1981 aacccgcgga tccgctcgag ggtctgcgga tcggagaccg gatgcccgcc cgcatcctcg
     2041 tagcagaagc cccgaccgcg gcgacgttgg tgcagccccg gctgtcccgc ttgtacccgc
     2101 cggatgcgtg gcacccctcg ctcctcccgc tcgatgatcc tcaggcgatg ccggtcgatg
     2161 gccggcccgc agagcgggag cgcccgctcg tccgtgagga ctacccgcca ccccgcccgc
     2221 cgaacccacc gaggcggtgt gggtccctgc agatgggtac gctgatcagg agacgtggcc
     2281 acgcccatcg tggcccgccg gtgggtctcc gatcgtggga gcgaggagga gggcgtgatc
     2341 cggttcggcc tgcagatccc gaacttcacg tatccgggcg tggacgatgc ggacctgttc
     2401 gagcgcatcg cccagatcgc caccaccgcc gagtccgccg ggttcgattc ggtgtgggtg
     2461 atggatcacc tgtaccagat ccccagcgtg ggaccgcgta cggacccgat gctcgaggcc
     2521 tacacgctgc tcggggcgat cgcggcccgg acgtcccggg tgcagctcgg cacgatggtc
     2581 accggggtca cctaccggaa cccggccctg ctggcgaaga tcgtcaccac actggacgcg
     2641 gtctcctctg gccgtgccat cctggggatc ggagccgcct ggaacgagga cgagcaccgc
     2701 ggctacggct tccgcttccc gcccgccggt gagcgcctgg accagctgga ggaggcgctg
     2761 cggatctgcc gagcgatgtt cacgcagcag gcaccgtcgt tccagggcga gcactaccgg
     2821 atccacgagg ccctgaacgt cccccgcccc atccgcccga acggcatccc catcctggtg
     2881 ggtggcagcg gtgagaagcg cacgctccgg ctggtggccc agtacgccga cgcctgcaac
     2941 ctcttcggag acctggacac gatccggcac aagctcgagg tgctggacgc gcactgcgag
     3001 gctgtgggcc gagacccggc cgagatcacc aagacccgtc taggggggtt ggtgatcgca
     3061 cccagcaagg aggaggcgga gcgcacgggc gccgaggccg cggccaggcg ggggacggat
     3121 ctggagcggt accgcgcgta cgccatgatc ggcgacccgg aca
//
LOCUS       DASQQQ010000171         3374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_7886, whole genome shotgun sequence.
ACCESSION   DASQQQ010000171 DASQQQ010000000
VERSION     DASQQQ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7886"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VE646_06845"
     CDS             complement(<1..742)
                     /locus_tag="VE646_06845"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HZD79741.1"
                     /translation="MKSYRVDLRSDTVTKPSEAMRRAMAEAEVGDDWYGDDPTVNRLQ
                     ERAAEVTGKEAALYVPTGTMANQIGLRLHVHGSGHLVVAESHAHVATTEVMTSAVLSG
                     IAYRTIPGNARGQITAEQVREALQPDAYYDVEMVDLLAVENTHGHAGGTVMPVEELRA
                     IRKVSQEVGVPIHMDGARIFNASAASGTDVADYCAEVATMMFCVSKGLGAPIGSLVCG
                     PAEPIREARRLKILFGGAWRQAGVMAAAG"
     gene            complement(789..1937)
                     /locus_tag="VE646_06850"
     CDS             complement(789..1937)
                     /locus_tag="VE646_06850"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HZD79742.1"
                     /translation="MKVGILTGGGDVPGLNACIKAATLRAVEEGHEVVGIRRGWAGIL
                     ECNPEDPVSVERNSIRLDPSVVRTIDRSGGTFLHTSRTNPAKVRPSEEPEFLHEPAED
                     DTPRDHTPHALKVIERMGIDALIPIGGDDTLSFALRLHDEGLPVVAVPKTMDNDVHGT
                     DYCIGFSTAVTRTVNFIHQLRTSAGSHERLAVVEVFGRYSGETSLVSAYLAGVERAVI
                     SEVPFDIQRLADLLMADRAGNPSRYAMMTISEGASMVGGEMVLGGQEDAYGHRKLGGI
                     GQVTGEALRQLTGEGIIYQQLGYLMRSGSPDSLDLMVAINYAVMAADLLVEREFGRLV
                     ALRNGNYVNVPLPVVRQGVKRVDVGALYDTEQYRPKIRHVDGKPMFLY"
     gene            complement(1987..3027)
                     /locus_tag="VE646_06855"
     CDS             complement(1987..3027)
                     /locus_tag="VE646_06855"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017565081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HZD79743.1"
                     /translation="MEGASLAKRIGILTGGGDCPGLNAAIRAVVRKARGDLGWEVLGF
                     RDGWRGVIEADVRPLTEESVGGILYRGGTILGSSGMNPYSQGGIDRVRENFRDLEVDG
                     LVVIGGEGTLGAAARMAEEGLPVVGVPKTIDNDLSGTDFCIGFQTAVQVVTEAIDRLH
                     TTAESHNRLMLVEVMGRSAGWIAIHGGIAGGADMILIPERPFDLTEVSHHLRRRHLRG
                     HSFSIVVVAEGATPVPGTFELPEYPVDERGFIRYGGIAQALAPEIERLTGYETRVTVL
                     GHVQRGGTPCADDRVLATRFGIGVVDLVDRGAWGTMVGISGNEVVEVPLAEAAKVRPV
                     PGSLYRAAEVFF"
     gene            3181..>3374
                     /locus_tag="VE646_06860"
     CDS             3181..>3374
                     /locus_tag="VE646_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79744.1"
                     /translation="MSPAHAGSIDQRLSTARGERRAALARLRTLHAELRDLLTRYARL
                     ERRAGRASLRLVDASRAVQEA"
BASE COUNT          477 a         1276 c         1108 g          513 t
ORIGIN      
        1 gcccggccgc cgccatgacc ccggcttgcc gccacgcgcc gccgaacagg atcttcaggc
       61 gccgggcctc tcggatcggc tcggccgggc cgcacaccag cgaaccgatc ggtgccccca
      121 ggcccttgga gacgcagaac atcatcgtgg cgacctcggc gcagtagtcg gccacgtcgg
      181 tgccggatgc cgccgaggcg ttgaagatcc gcgccccgtc catgtgtatg ggaacaccga
      241 cctcctgcga gaccttccgg atcgcacgca gctcttccac cggcatcacg gtgccgccgg
      301 catggccgtg cgtgttctcc acggccagca gatcgaccat ctcgacgtcg tagtacgcgt
      361 ccggctgcag cgcctcccgg acctgctcgg cggtgatctg gcccctggcg ttcccgggaa
      421 tcgtccggta ggcgatgccc gagagcaccg ccgaggtcat cacctccgtc gtggccacgt
      481 gggcgtggga ctcggccacc accaggtgcc ccgacccgtg gacgtggagc cgcagcccga
      541 tctggttggc catcgtgccg gtgggcacgt agagggcagc ctccttgccg gtgacctcgg
      601 ccgcccgctc ctggagccgg ttcaccgtgg gatcgtcgcc gtaccagtca tcgccgacct
      661 cggcctccgc catggcgcgg cgcatcgctt ccgagggctt cgtgaccgtg tccgaacgca
      721 gatcgacccg gtaggacttc acgatcaggc cagtatagga ggccctcccg cgccggccga
      781 tccggcgctc agtacaggaa catcggcttg ccgtccacgt ggcggatctt gggccggtac
      841 tgctcggtgt cgtagagcgc tcccacgtcc acgcgcttca ccccctggcg gaccaccggg
      901 agcgggacgt tcacgtagtt gccgttgcgc agggccacca gccggccgaa ctcccgctcc
      961 accagcaggt ccgcggccat caccgcgtag ttgatcgcca ccatcaggtc gagcgaatcg
     1021 gggcttcccg agcgcatcag gtaccccagc tgctggtaga tgatcccctc gccggtgagc
     1081 tggcgcaggg cctcgcccgt gacctggccg atgcccccga gtttgcggtg cccgtacgcg
     1141 tcctcctgac cccccaggac catctcaccg cccaccatgc tggcgccctc ggagatcgtc
     1201 atcatcgcgt agcggctggg gttgccggcc cggtcggcca tcagcaggtc cgccagcctc
     1261 tggatgtcga agggcacctc ggagatgacg gcccgctcca cgcccgccag gtacgccgac
     1321 accagggagg tctcgccgct gtaccggccg aacacctcga ccacggccag gcgctcgtgc
     1381 gacccggcgc tggtccgcag ctgatggatg aagttcaccg tgcgggtgac ggcggtcgag
     1441 aacccgatgc agtagtcggt gccgtggacg tcgttgtcca tcgtcttggg gaccgccacc
     1501 accggcaggc cctcgtcgtg caggcgtagg gcgaaggaca gggtgtcgtc gccgccgatg
     1561 gggatcagcg cgtcgatccc catccgctcg atcaccttca gcgcgtgggg tgtgtggtcg
     1621 cgcggcgtat cgtcctccgc gggctcgtgc aagaactccg gctcctccga ggggcggacc
     1681 ttggcggggt tcgtcctgga ggtgtgcagg aacgtgccgc cgctgcggtc gatcgtccgc
     1741 accacggagg ggtccagccg gatcgagttc cgctcgacgg agaccgggtc ctccgggttg
     1801 cactcgagga tgccggccca gccgcgccgg atcccgacca cctcgtgccc ctcctccacc
     1861 gcgcggagcg tggcagcctt gatgcacgcg ttcagccccg ggacgtcccc tccgcccgtc
     1921 aggatgccga ccttcatgag cgctccacct tcccgcgccg gacccaccgg cgtccatccc
     1981 tcgatgctag aagaagacct cggcggcccg atacagggat ccgggcacgg gccgcacctt
     2041 ggccgcctcg gccaggggca cctcgaccac ctcgttgccg ctgatcccca ccatcgtgcc
     2101 ccaggccccg cggtccacca ggtccaccac cccgatcccg aaccgcgtgg caaggacccg
     2161 atcgtcggcg cagggggtcc cgccgcgctg cacgtgcccc aggaccgtga cccgggtctc
     2221 gtacccggtg agccgctcga tctccggcgc cagcgcctgg gcgatccccc cgtagcggat
     2281 gaagccccgc tcgtccaccg ggtactcggg gagctcgaac gtgccgggca ccggcgtcgc
     2341 cccctcggcc accaccacga tcgagaagga gtgaccccgc aggtgtcgcc gccggaggtg
     2401 gtggctcact tcggtcaggt cgaagggtcg ctcggggatg aggatcatgt cggcgccccc
     2461 ggcgatgccg ccgtggatgg cgatccagcc ggcgctccga cccatcacct ccacgagcat
     2521 cagccggttg tggctctcgg ccgtcgtgtg caggcgatcg atggcctccg tcaccacctg
     2581 gaccgccgtc tggaagccga tgcagaagtc cgtcccggac aggtcgttgt cgatggtctt
     2641 gggcacgccc accaccggca gcccttcctc ggccatccgc gcggccgcgc cgagggtgcc
     2701 ctcgccgccg atcaccacca ggccgtcgac ctccaggtcc cggaagttct cacggacccg
     2761 gtcgatccct ccctgcgagt aggggttcat gccggagctg cccaggatcg tcccgccccg
     2821 gtacaggatc ccgcccacgg actcctccgt gagtggacgg acgtccgcct cgatgacccc
     2881 ccgccaaccg tccctgaagc ccaggacctc ccaccccagg tcccccctcg ccttgcggac
     2941 gacggcgcgg atcgccgcgt tcaagccggg acagtctccg ccgcccgtca ggatgccgat
     3001 tcgcttggcc agggatgcac cctccatcaa ccgggctgag gttcgagcct gttgcccatc
     3061 cggtcgagtc gattctaggc tgggggcggg ccaccggccc acggaggagg agacgacacg
     3121 gcacgcagag cgcggggaag agcgatcgcg gcgatcgctg cgctgtccat cctggcggcc
     3181 atgtcgccgg cccacgcggg gtcgatcgat caacgactct cgacggctcg gggcgagcgg
     3241 cgggcggccc tggcgcgcct gcggaccctg cacgccgagc ttcgcgacct gctgaccagg
     3301 tacgcccgtc tggaacgccg ggccgggcgt gcctccctcc ggctggtcga tgcctcccgg
     3361 gccgtgcagg aagc
//
LOCUS       DASQQQ010000172        10820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_368325, whole genome shotgun sequence.
ACCESSION   DASQQQ010000172 DASQQQ010000000
VERSION     DASQQQ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10820
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368325"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VE646_06865"
     CDS             <1..196
                     /locus_tag="VE646_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZD79745.1"
                     /translation="VWYITPVPDAKAIIANQLDDPAVAHSQLVFPSNATYQKAHDYYT
                     FKNFQDEQQWNDIFQPIIEG"
     gene            160..1065
                     /locus_tag="VE646_06870"
     CDS             160..1065
                     /locus_tag="VE646_06870"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD79746.1"
                     /translation="MERHLPANHRGLSARKAPFGRRLAPYLLSAPGGIWLLVFFVIPF
                     FVMFSVSLQTGNIDIGFTLTWHIAEYWQAVTLYHAFIVRSLWYASVVTVLTLIVGYPV
                     AYWIAFHAGRRKTTYLFLLLLPFFVSFVIRTLSWGFILADQGVVLGTLKRVHILPQDF
                     HVIATSTAVIAGIAYNSLPFTVLPLYVSLEKIDRSVLEAAHDLYANNVAVFRKVILPL
                     TVPGIFAAFLLTFIPAAGDYVNATILGSTGTRMVGNIIQDLYLGSAFNYPMASALSFI
                     LMAILLVGILAYARLLGTRSIEEFI"
     gene            1065..1937
                     /locus_tag="VE646_06875"
     CDS             1065..1937
                     /locus_tag="VE646_06875"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD79747.1"
                     /translation="MAIAEGPIALGEAQEQRPELEPLRPPNRRWTRFILPAFTKLVMI
                     YLLVPIAIMILYTFNKTLGGTPKVTFRWNGFTTEWYRQLLGVPDLTAALKNSILIAFT
                     STVVATALGTLMALALVRYRYRGQTATDFVMFLNISQPEVVMGAALLSLFVTINFPRG
                     LLSIFIAHVMFNIPIVAVTVRARLAGYDTTIEDAARDLYADALRTFTKVTLPLIWPGI
                     LAGALLAFALSIDDFVVTQFVAGTTVTFPLWVFGAVKVGIPPQVFVWGTLIFAVGVVV
                     ALFNVILQQRKQPG"
     gene            complement(1941..3677)
                     /gene="menD"
                     /locus_tag="VE646_06880"
     CDS             complement(1941..3677)
                     /gene="menD"
                     /locus_tag="VE646_06880"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HZD79748.1"
                     /translation="MSEGDVAFARMRAVASALAWTGVRHACVSPGSRSTPLALALWRE
                     PRIQVHVHLDERASAFFALGVGKATGLPAAVACTSGTAAAELLPAVVEASMSRTPLLL
                     LTADRPPELRGVGANQTIDQIELYGRYVRWSIDAPVPEAADDLEGWRSLAVEAAARSF
                     GHHPPGPVHVNLPFREPLLPVAVPDPTERVPAGSTPLPAAAPPRPRSEDLRRLAELTS
                     GGVERGLVYAGSLRVAAGGVLDAADALGWPVIAEPTSGLRVPGTLDAGQLLVADQRFR
                     AEHRPELVLQLGAAPTSRAAQALTSETERLVIVDPDDLVADPGRRADLRLVADPNLVA
                     RELTGRMSRRAGDGWLAHWREGDQRTGEAVDALLGGWEEPFEGRIARDLAEALPQGSL
                     LCVGSSMPVRDLDAFMRPRDGLRVLANRGASGIDGFVSTVLGAGVAGRPTFALLGDLT
                     LVHDVGSLLWSARRGHDAVLVIPNNGGGAIFSSLAQRRLPSEELEELFTTPHRLDLGA
                     VARAAGARHVRVERAVDLTPAVLEAHRGGGVWFVEVSVDPGRDLTRRAAVREAVARAL
                     REDAGLPEPSAG"
     gene            complement(3674..4582)
                     /locus_tag="VE646_06885"
     CDS             complement(3674..4582)
                     /locus_tag="VE646_06885"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HZD79749.1"
                     /translation="MAASRLAVWWHGARPRTLAAGVMPVIVGTAAAGRASWWRFGAAL
                     LVALGLQIGVNYANDYHDGVRGIDTEHRTGPPRLTSSGMAAPASVAAAALACIGVAGV
                     AGLALAVATSLWLIPVGALAMVALWLYSGGPRPYAGLGLGEVMVFLFFGLMATAGTAY
                     VQAERVPAAAWWASVVMGLLAVAILVANNLRDIPTDEATGKRTLAVRLGDRRTRVLYR
                     AVVVLAFAVAVTGVIAGIVASGAGLSQWALLSLAAWVVAIRPMELVGRARGGELVEVL
                     VGTASVQAAFGALLALGLWIAGVTAA"
     gene            complement(4588..5412)
                     /gene="menB"
                     /locus_tag="VE646_06890"
     CDS             complement(4588..5412)
                     /gene="menB"
                     /locus_tag="VE646_06890"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017570490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HZD79750.1"
                     /translation="MIGLMDWQRSGEYQDIRYESAGGIAKITIDRPEVRNAFRPTTLF
                     ELSRGFEAARDDPTIGVIVLTGEGPLAFCSGGDQRVRGDDGYKDPRGIGRLNVLDLQI
                     QIRRLPKPVIAMVAGYAIGGGHVLHLVCDLTIAADNAKFGQTGPKVGSFDAGYGSGLL
                     AATIGLKRAKEIWFLCEQYDAQTALQWGLVNRVVPLETLEEETVRVCRRILQMSPLAL
                     RMIKGGMHAWTDGLAGIQQLAGDSTLLFYLSDEAQEGRDAYLEKRPPDFSRFPKRP"
     gene            5455..6642
                     /locus_tag="VE646_06895"
     CDS             5455..6642
                     /locus_tag="VE646_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZD79751.1"
                     /translation="MARSGNVGVGPGDVVAVEMPLGSAWPGLVEAVWGAGAALMPVDH
                     RLPPPARDRLLAAARPTVRVDPTGWHRLAQGSPVDDAVTLVVATSGTSGAQKLALFTR
                     QAVEAAVLASAQALGAARDDGWLACLPPAHIGGLLVVLRASILGAPLVCLPAFDAAQV
                     AAATSSRFTSLVPLMLRRLLDAEARLGHLRAILVGAGGMDAGLRERAGAAGVTAVQTY
                     GLTESCGGVVYDGLPLPGIRLRIDPETSAIELAGPTLMLEYRFDERATAAAFTPDGWL
                     RTADGGRLERDGRLHPLGRLDERIDTGGERVWPDEVEGALRGHPRIVEVAVVGRPDRE
                     WGERVVAFVVPTDPADPPSLDEIRGHVSLSLPRYMAPRQLVLRRGPLPRSASGKVRRG
                     DLA"
     gene            6729..7670
                     /gene="trxB"
                     /locus_tag="VE646_06900"
     CDS             6729..7670
                     /gene="trxB"
                     /locus_tag="VE646_06900"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HZD79752.1"
                     /translation="METIVADVRNVIILGSGPAGYTAALYAGRADLEPLVLKGLDAGG
                     QLMLTTEVENYPGFPDAVLGPELMEAMEKQAARFGTELLHQTATRVDLSHWPFSVWAG
                     DQEWSARSLIAATGASAKWLGVPGEERLRGRGVSACATCDGFFFRDRELVVVGGGDTA
                     MEEATFLTKFASKVSIVHRRDEFRASKIMQERALVNPKISVIWDSVVDEILGQDAVTG
                     VRLRNVKTGETSEPPTDGVFMAIGHTPNTSLFEGQLELSNGYIVVQEPTTRTSVQGVF
                     AAGDVTDVIYRQAVTAAGQGCKAAIDAERFLLEHEGT"
     gene            7799..8119
                     /gene="trxA"
                     /locus_tag="VE646_06905"
     CDS             7799..8119
                     /gene="trxA"
                     /locus_tag="VE646_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZD79753.1"
                     /translation="MGDVTDQDFDSRVLGSSTPVLVDFWAEWCVPCHMVSPVVEEIGR
                     EKDDALQVVKLNIDENPEITRRYGIMSIPTLMLFVSGQERARLVGARGKDALLREIDP
                     HIAA"
     gene            8181..9299
                     /locus_tag="VE646_06910"
     CDS             8181..9299
                     /locus_tag="VE646_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HZD79754.1"
                     /translation="MKIIRRGDAGEAVRDIQHRLLDLGLRIEPGELEGRFGPATEQAI
                     RDFQASRGLPADGLVGPDTWGQLVEAGYRLGDRVLYLRSPAFRGDDVRELQRRLNALG
                     FDAGREDGILGTATQVAVVEFQRNVGEQPDGIVAMGTVDALRRVRSSVGSTSRALVRE
                     AEAMRRMARALSGCRVAVDPGHGPDEPGYTSRTGMAEGELTLPIAEVLCEELAARGAI
                     PFLLRPAHEDPAPSERAHDANDLNADVCVSIHLGGADPGAQGPVCSYFGTTDTYSPAG
                     QRLAEIVTEALADVGIGPGTTQRLAVAMLRETRMPAVQVEPCALTDPASEPLITDPSW
                     RRRVAVALADAVQRFVEGAGAQAATGAASARVGPSPLP"
     gene            9562..10515
                     /locus_tag="VE646_06915"
     CDS             9562..10515
                     /locus_tag="VE646_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HZD79755.1"
                     /translation="MRFCLMIEGQEGPTWQQWLDLAAACERLGFEALFSSDHYYSVMD
                     APGRGSHDAWTLLAGLAARTEHLRLGTLVSPVTFRLPTVLAKVATTVDQISAGRVELG
                     MGAGWWEEEHRTHGFPFPPAGQRMEMLAEQLQIVHGLFSEDPFTFQGRHYQLERCTFL
                     HKPVQHPHPPIIVGGTGGPLMASLVARWADEFNTVGGTPDEVRTRFERVRQAVTDRGR
                     DQSEVVTSFMTWFYVGEDEAAFRANVERARARDPKAGDFDAYLAELQRDCITGTPERA
                     AERLSEYAAAGVQRVMLNHELYEDVGALELLAEEVFPRVEG"
     gene            complement(10512..>10820)
                     /locus_tag="VE646_06920"
     CDS             complement(10512..>10820)
                     /locus_tag="VE646_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79756.1"
                     /translation="RAMERIREAGAGRCLPGHGPAGPMLPRNTDPVERHLRWVLDRAA
                     EAVVRGLSGAALREDLRRRFEDQRSRPGGVDFPVRIPGFLEVTASAAERDVRASAPRP
                     "
BASE COUNT         1569 a         3959 c         3743 g         1549 t
ORIGIN      
        1 ggtctggtac atcacccccg ttccggacgc gaaggcgatc atcgccaacc agctggacga
       61 ccctgccgtg gcccacagtc agctggtctt cccgagcaac gccacgtacc agaaggcgca
      121 cgactactac acgttcaaga acttccagga cgagcagcag tggaacgaca tcttccagcc
      181 aatcatcgag ggctgagcgc caggaaggct cccttcggtc gacggctggc cccgtacctg
      241 ctgagcgcgc ccggcgggat ctggctcctg gtcttcttcg tgatcccgtt cttcgtgatg
      301 ttctccgtct cgctgcagac cgggaacatc gacatcgggt tcacgctcac gtggcacatc
      361 gccgagtact ggcaggccgt caccctgtac cacgccttca tcgtgcgatc gctctggtac
      421 gcgagcgtgg tgaccgtgct gaccctgatc gtgggctacc cggtggccta ctggatcgcg
      481 tttcacgccg ggcgccggaa gaccacgtac ctgttcctgc tcctgctgcc gttcttcgtc
      541 tccttcgtga tccgcacgct ctcgtggggc ttcatcctcg cggaccaggg cgtggtgctg
      601 ggcacgctga agcgggtgca catcctgcca caggacttcc acgtgatcgc cacctccacg
      661 gcggtgatcg cggggatcgc ctacaactcc ctgcccttca cggtgctccc gctctacgtg
      721 tccctggaga agatcgatcg ctccgtgctc gaagcggcac atgatctgta cgcgaacaac
      781 gtcgccgtgt tccgcaaggt gatcctcccg ctcaccgtcc cggggatctt cgcggccttc
      841 ctgctcacgt tcatcccggc cgccggcgac tacgtgaacg cgaccatcct ggggagcacc
      901 ggcacccgga tggtgggcaa catcatccag gacctgtacc tgggcagcgc cttcaactac
      961 ccgatggcgt cggccctctc gttcatcctg atggccatcc tgctggtggg gatcctcgcc
     1021 tacgcgcggc tgctcggcac gcgcagcatc gaggagttca tctgatggcg atcgccgaag
     1081 gtcccatcgc gctcggcgag gcgcaggagc aacggccgga actggagccg ctgcggcctc
     1141 ccaaccggcg gtggacgcgg ttcatcctgc ccgccttcac caagctcgtg atgatctacc
     1201 tgctggtgcc gatcgccatc atgatcctgt acacgttcaa caagaccctg gggggcaccc
     1261 cgaaggtcac cttccgctgg aacggcttca ccaccgagtg gtaccgccag ttgctggggg
     1321 tccccgatct gacggcggcc ctgaagaact cgatcctgat cgcgttcacc agcacggtcg
     1381 tggccaccgc cctgggcacg ctcatggctc tcgccctggt gcggtaccgg taccggggcc
     1441 agaccgccac ggacttcgtg atgttcctga acatctccca gcccgaggtg gtgatggggg
     1501 cggcgctgtt gtccctgttc gtcaccatca acttcccgcg agggctcctg tcgatcttca
     1561 tcgcgcacgt catgttcaac atcccgatcg tggcggtgac cgtccgggcg cggctggccg
     1621 gctacgacac cacgatcgag gacgccgcca gggacctgta cgccgatgcc ctgaggacgt
     1681 tcaccaaggt gaccctgccg ttgatctggc cggggatcct ggccggggcc ctgctggcgt
     1741 tcgccctgtc gatcgacgac ttcgtggtca cccagttcgt ggccggcacc accgtgacct
     1801 tcccgctgtg ggtcttcggc gcggtcaagg tcggcatccc gccgcaggtc ttcgtgtggg
     1861 gcacgctgat cttcgcggtg ggggtggtgg tggcgctctt caacgtgatc ctgcagcagc
     1921 ggaagcagcc gggctgagcc tcaacctgcg gagggctcgg ggaggccggc atcctccctc
     1981 agcgctcggg ccaccgcttc gcggaccgcc gcccgccggg tcaggtcccg ccccgggtcc
     2041 accgatacct ccacgaacca cacgccgccc ccccggtgcg cctcgagcac ggcgggggtg
     2101 agatccaccg cccgctccac ccggacgtgc cgagcgccgg cggcccgggc caccgccccc
     2161 agatcgaggc ggtgcggcgt ggtgaacagt tcctccagct cctccgaggg cagccggcgc
     2221 tgggccagcg aggagaagat cgctcccccc ccgttgttcg ggatcaccaa gacggcatcg
     2281 tggcctcgac gggcgctcca cagcagcgaa cccacatcgt gcaccagcgt gaggtcgccg
     2341 agcagcgcga aggtcggcct ccccgccacg cccgccccca gcaccgtgga gacgaacccg
     2401 tcgatcccgc tggctccccg gttcgccagc acccggagcc catcgcgggg acgcatgaac
     2461 gcatcgagat cgcgcaccgg catgctcgac cccacacaca ggagcgatcc ttgggggagc
     2521 gcctcggcca ggtcccgcgc gatccggccc tcgaagggct cctcccagcc acccaggagc
     2581 gcgtccaccg cctcccctgt ccgctggtcc ccctcccgcc agtgggccag ccacccgtcc
     2641 ccagcgcggc ggctcatgcg tccggtcagc tcgcgcgcga ccaggttcgg gtcggccacc
     2701 aggcgcaggt cggcccgccg acccgggtcg gccaccaggt cgtcggggtc cacgatcacc
     2761 aggcgctcgg tctccgaggt cagcgcctgg gccgcccggg acgtcggtgc cgctcccaac
     2821 tggagaacca gctccggccg atgctccgcc cggaagcgct ggtcggccac cagcagctgg
     2881 ccggcgtcga gcgttccggg gacccgaagg cctgacgtcg gctcggcgat caccggccac
     2941 ccgagcgcat ccgccgcatc gaggaccccg ccggcggcga cccgcaggga tccggcgtac
     3001 acgaggccac gctcgacccc gccgctcgtc agctcggcca ggcgacggag gtcctccgac
     3061 ctcggccgcg gcggggccgc ggccggtagg ggggtcgatc cggccgggac gcgctcggtc
     3121 ggatccggca cggccacggg caggagcggc tcacggaagg gcaggttcac gtgcacgggg
     3181 ccgggtggat ggtggccgaa gctccgcgcc gccgcctcca ccgccaggct ccgccagccc
     3241 tccagatcgt cggccgcttc gggtacggga gcgtcgatgg accaacgcac gtaccgaccg
     3301 tagagctcga tctggtcgat ggtctggttc gcgcccacgc ctcggagctc cggaggccgg
     3361 tccgccgtga gcaggagcaa cggcgtccga gacatcgacg cctccaccac cgccggcagg
     3421 agctcggcgg ccgcggtgcc gctggtgcag gccaccgccg ctggcagccc ggtggccttg
     3481 cccacgccga gcgcgaagaa ggccgaggcc cgctcgtcca ggtggacgtg gacctggatc
     3541 ctcggctcgc gccagagggc cagggccagg ggcgtcgaac gggagccggg ggaaacgcag
     3601 gcgtgccgga cgccggtcca ggcgagcgcg ctcgcgaccg cccgcatacg tgcgaacgcg
     3661 acgtccccct cactcacgcg gccgtgaccc ccgcgatcca gagtccgagc gccagcagcg
     3721 caccgaaggc ggcctgcacc gacgcggtgc ccaccaggac ctcgaccagc tcgcctcccc
     3781 gcgcccgccc caccagctcc atgggccgga tcgccaccac ccatgccgcc aacgacagga
     3841 gggcccactg ggagagaccc gctcccgaag ccacgatgcc cgcgatcacc ccggtcaccg
     3901 ccaccgcgaa ggccaggacc accacggccc ggtacaggac gcgggtccgg cgatccccca
     3961 atcggaccgc cagcgtgcgc ttgccggtgg cctcgtcggt ggggatgtcg cgcaggttgt
     4021 tggccaccag gatcgccacc gccagcagtc ccatcaccac cgacgcccac caggcggcgg
     4081 ccggcacgcg ctcggcctgc acgtaggccg tgccggcggt agccatcaga ccgaagaaca
     4141 ggaagaccat gacctcgccc agaccgaggc cggcgtaggg ccgcggcccg cccgagtaca
     4201 gccacagcgc caccatggcc agggcgccga cggggatcag ccacagcgac gtggccacgg
     4261 ccagggcgag ccccgccacg ccggccaccc cgatgcaggc cagggcggcc gccgccaccg
     4321 aggccggagc ggccatcccc gaggaggtca acctcggcgg tccggtgcgg tgctcggtgt
     4381 cgatcccccg cacgccgtcg tggtagtcgt tggcgtagtt cacgccgatc tgcagcccga
     4441 gggcaaccag cagggcggcg ccgaaccgcc accaggaggc ccgaccggcc gccgccgtcc
     4501 ccacgatcac cggcatgacg ccggcggcga gcgttcgagg gcgtgcgccg tgccaccaga
     4561 cggccaggcg gctcgcggcc atcgcggtca cggccgcttg gggaacctcg agaagtccgg
     4621 ggggcgcttc tccaggtagg cgtcgcgacc ttcctgggcc tcgtcggaca ggtagaacag
     4681 gagcgtcgag tcgcccgcga gctgctggat cccggccagg ccgtcggtcc acgcgtgcat
     4741 cccgcccttg atcatcctca gcgccagggg actcatctgc aggatccgac ggcacacccg
     4801 caccgtctcc tcctccagcg tctccagggg gaccacccgg ttgaccaacc cccactgcag
     4861 cgccgtctgg gcgtcgtact gctcgcacag gaaccagatc tccttggccc gcttcaagcc
     4921 gatcgtggcg gcgaggagac cgctcccgta cccggcatcg aacgacccca ccttgggacc
     4981 ggtctggccg aacttcgcgt tgtcggcggc gatggtgaga tcgcacacga ggtggagcac
     5041 gtggccgcca ccgatcgcgt acccagccac catcgcgatc accggcttgg gtagccgacg
     5101 gatctggatc tgcaggtcca ggacgttcag gcggccgatc ccccgggggt ccttgtagcc
     5161 atcgtcgccc cgaacccgct ggtccccacc cgagcagaag gccaagggcc cttccccggt
     5221 gaggacgatc acgccgatgg tcgggtcgtc gcgagctgcc tcgaagcccc gcgagagctc
     5281 gaacagcgtg gtggggcgga acgcgttgcg cacctccgga cgatcgatcg tgatcttggc
     5341 gatgcccccc gcgctctcgt agcggatgtc ctggtactca cccgaccgct gccaatccat
     5401 cagaccgatc actcctagag cgttgactcg aacggttagg ctagcctatc tcaggtggcg
     5461 cggagcggga acgtcggggt cgggccgggc gacgtcgtgg cggtggagat gcctctcggg
     5521 tccgcgtggc cggggctggt ggaggcggtc tggggcgccg gtgcggcgct catgccggtc
     5581 gaccaccgcc tgccaccccc ggcgcgcgac cgtctgctgg ccgccgcccg ccccacggtc
     5641 cgagtcgacc ccaccggatg gcaccggctc gcccagggga gccccgtgga tgatgcggtg
     5701 accctggtcg tggccacctc cggcacatcg ggggcgcaga agctggcgct gttcacacgg
     5761 caggccgtgg aggccgccgt gctggcctcg gcgcaggccc tcggtgccgc ccgggacgac
     5821 ggctggctgg cctgcctccc cccggcgcac atcggcgggc tgctggtggt gctccgggcc
     5881 tcgatcctgg gtgcaccgct cgtgtgcctt ccggcgttcg acgcggccca ggtggcggct
     5941 gccacctcca gccggttcac ctcgctcgtg ccgctcatgc tccggcgcct gctggatgcg
     6001 gaggcccggc tggggcatct ccgagcgatc ctggtgggcg ctggcgggat ggacgccggc
     6061 ctgcgcgaac gcgctggggc cgccggggtc acggccgttc agacctatgg tctcaccgag
     6121 agctgcggcg gggtggtcta cgatggtctc cccctccccg gcatccgcct caggatcgat
     6181 cccgagacct ccgccatcga gctcgccggg cccaccctca tgctggagta ccgcttcgac
     6241 gagcgagcga ccgctgccgc cttcacacca gacggttggc tgcgaacggc ggacgggggc
     6301 cggctggagc gggacgggcg gctgcacccg ctgggacggc ttgacgagcg gatcgacacc
     6361 gggggcgagc gggtgtggcc cgacgaggtg gagggcgccc tccgagggca ccccaggatc
     6421 gtcgaggtcg ccgtggtcgg caggcccgat cgcgaatggg gcgagcgggt cgtggcgttc
     6481 gtcgtgccga ccgatccggc cgatcccccg agcctggacg agatccgcgg ccacgtgtcc
     6541 ctctcgctgc ctcggtacat ggcgcctcgc cagctcgtcc tgaggcgcgg cccgctcccc
     6601 cgctcggcat cgggcaaggt ccgtcgcggg gacctggctt gagcaccccg ccgtgggcca
     6661 cggatgcggc acgggaggcg tcggcggccg cgtggaagga atagggagcg gttatgagct
     6721 atgggagaat ggagacgatc gtggccgacg tacgcaacgt gatcatcctg ggctcgggtc
     6781 ccgccggcta cacggccgcg ctgtacgcgg ggcgcgccga cctggagccg ttggtgctca
     6841 aggggctgga cgccggcggg cagctcatgc tcactaccga ggtcgagaac tacccggggt
     6901 tccccgacgc cgtcctcggt ccggagctga tggaggccat ggagaaacag gcggcccggt
     6961 tcgggaccga actgctgcac cagaccgcca cccgcgtgga cctgtcgcac tggcccttct
     7021 cggtgtgggc cggcgaccag gagtggtcgg cccggtccct gatcgcggcc accggggcca
     7081 gcgccaagtg gctcggcgtg ccgggcgagg agcggctccg gggacgcggc gtgtccgcct
     7141 gcgccacctg cgacggcttc ttcttccggg accgggagct cgtcgtcgtg ggcggcggcg
     7201 acaccgcgat ggaggaggcc accttcctca cgaagttcgc ctccaaggta tcgatcgtgc
     7261 accggcgtga cgagttccgg gcctcgaaga tcatgcagga gcgggccctc gtcaacccca
     7321 agatctccgt gatctgggac agcgtggtcg acgagatcct gggccaggat gcggtcaccg
     7381 gcgtgcggct gcgcaacgtg aagaccggcg agacgtccga gccaccgacc gacggggtgt
     7441 tcatggccat cgggcacacg ccgaacacct cgctgttcga gggtcagttg gagctgtcga
     7501 acgggtacat cgtggtacag gagcccacca ccaggaccag cgtgcagggc gtgttcgcgg
     7561 cgggcgacgt caccgacgtg atctaccgtc aggcggtgac ggcggccgga cagggctgca
     7621 aggcggccat cgatgccgag cgcttcctcc tcgagcacga gggcacctga ggacggcgag
     7681 ccgggcacga ccgcgggccc gggaacgcgg tggccgcgcg gagcatcgtg aggcaacgcc
     7741 gacggccgga tgcggccgga gggacgagag gcgacagaaa ggatcgaacg tggggctgat
     7801 gggcgatgtg acggaccagg acttcgacag cagggtgctg ggttccagca cccccgtgct
     7861 cgtggacttc tgggcggagt ggtgcgttcc ctgccacatg gtctctcccg tggtggagga
     7921 gatcggccgg gagaaggacg atgcgctcca ggttgtgaag ctcaacatcg acgagaaccc
     7981 tgagatcacg cgccgctacg gcatcatgag catccccacc ctgatgctct tcgtgagcgg
     8041 tcaggagcgc gcccgcctgg tcggggctcg gggcaaggac gccctgcttc gagagatcga
     8101 tccgcacatc gcggcctgac acgcgccggg gccgtgctcc ccggttgccg cggaccggcg
     8161 gtcctaccga tactccacgc gtgaagatca tccgacgtgg tgatgccggc gaggccgtgc
     8221 gcgacatcca gcaccggctc ctggacctcg gcctccggat cgagcccggt gagctggagg
     8281 gccggttcgg cccggccacc gagcaggcga tccgggattt ccaggcctcc cgcgggctcc
     8341 ccgccgacgg gctggtcggg cccgacacct ggggccaatt ggtcgaggcc gggtaccggc
     8401 tgggggatcg ggtcctgtac ctgcgctccc cggccttccg aggcgacgac gttcgggagc
     8461 tccagcggcg gctgaacgcg ctcggcttcg atgccgggcg cgaggacggc atcctgggca
     8521 cggccaccca ggtcgccgtg gtcgagttcc agcgcaacgt tggcgagcag cccgacggca
     8581 tcgtggccat gggcaccgtg gacgcccttc gccgggtccg ctcgagcgtc ggcagcacca
     8641 gccgggcgct ggtccgggag gcggaagcga tgcggcggat ggcccgggcc ctctcgggct
     8701 gccgggtggc cgtcgacccc ggccacggtc cggacgagcc cgggtacacg agccggaccg
     8761 ggatggcgga gggtgagctc accctgccga tcgccgaggt cctgtgcgag gagctcgccg
     8821 ctcggggagc gatccccttc ctgctccgcc ccgctcacga ggacccggcg ccgagcgagc
     8881 gggcgcacga cgccaacgac ctgaacgccg acgtgtgcgt ctcgatccat ctgggcggtg
     8941 ccgatcctgg cgcgcaggga ccggtctgct cctacttcgg caccaccgac acctactccc
     9001 ccgccgggca gcgcctggcc gagatcgtca ccgaggcgct ggccgacgtc gggatcggcc
     9061 ccggcaccac gcagcgcctg gcggtggcca tgctccgcga gacgcgcatg ccggcggtgc
     9121 aggtcgagcc atgcgccctc accgatcccg ccagcgaacc cctgatcacc gacccctcct
     9181 ggcggcgccg ggtcgccgtc gccctggcag atgcggtgca gcgcttcgtg gagggagccg
     9241 gtgcccaggc cgccaccggc gcggcctcag cgcgcgtcgg cccgtccccg ctcccgtgag
     9301 ctgctgacgg ccgcacgccc ggccagcacg atcatcacca gccccaggag cccgccgagg
     9361 aggagcgcac ggccggcgct ctccacctcg gccctcgaga tggccctcga cggggcgcct
     9421 ctccgcatcc gccggtggct cgctaccccg gctccggcac ctcctcgggt gcgaccctca
     9481 cccactggct cggtccagct tgtcaggacg caccatggaa ggattgtagg aggctcgatc
     9541 caccgaccat gcggaggcga catgcggttc tgtctgatga tcgagggtca agaaggcccc
     9601 acctggcagc agtggctcga cctggccgcc gcgtgcgagc ggctgggttt cgaggcgctg
     9661 ttctcctcgg atcactacta ctcggtgatg gatgctcccg gccgcgggtc ccacgatgcc
     9721 tggaccctcc tggccggcct ggccgcacgc acggagcacc tccggctcgg caccctggtc
     9781 tctcccgtca ccttccggtt gccgaccgtg ctcgcgaagg tggccaccac ggtggaccag
     9841 atctccgcag ggcgggtcga gctcggcatg ggcgccgggt ggtgggagga ggaacaccgc
     9901 acccacggct tccccttccc cccggccgga cagcgcatgg agatgctggc ggagcagttg
     9961 cagatcgtcc acggtctgtt ctccgaggac ccgttcacct tccagggccg gcactaccag
    10021 ctcgagcggt gcacgttcct gcacaagccc gtgcagcatc cccacccccc catcatcgtg
    10081 gggggcacgg gcgggccgct catggcatcg ctcgtggccc ggtgggcgga cgagttcaac
    10141 accgtgggcg gtaccccgga cgaggtgcgc accaggttcg agcgcgtgcg ccaagccgtc
    10201 accgaccgtg gccgcgacca gtcggaggtc gtcacctcct tcatgacgtg gttctacgtg
    10261 ggcgaggacg aggccgcctt ccgggccaac gtggagcggg ctcgggcccg cgatcccaag
    10321 gcgggcgact tcgatgccta cctcgccgag ctgcaacgcg actgcatcac cggcaccccc
    10381 gaacgcgccg cggagcgcct gtccgagtac gccgccgccg gcgtccagcg cgtgatgttg
    10441 aaccacgagc tgtacgagga cgtgggcgcg ctcgagctgc tggccgagga ggtcttcccc
    10501 cgggtggagg gctaggggcg gggggccgag gcccgcacgt cgcgttcggc ggccgaggcg
    10561 gtgacctcga ggaagccggg gatccggacg gggaagtcca ccccgcccgg cctggagcgc
    10621 tggtcctcga accgccgacg gaggtcctcg cggagggccg ctccgctcag gccccggacc
    10681 actgcctcgg ctgcccggtc caggacccag cgcagatgcc gctccacggg atcggtgttc
    10741 cggggcagca tgggtcccgc tggaccgtgg ccggggaggc accgcccggc ccccgcctcc
    10801 cggatccgct ccatggcgcg
//
LOCUS       DASQQQ010000173         3809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_69775, whole genome shotgun sequence.
ACCESSION   DASQQQ010000173 DASQQQ010000000
VERSION     DASQQQ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69775"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VE646_06925"
     CDS             <1..1081
                     /locus_tag="VE646_06925"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HZD79757.1"
                     /translation="FERQIKALRKSNNPTAPQQIASLRGEIGSRTSQKQLLPGLIVIQ
                     EPRAVPVVTTGFQPPSSRTTRVLIAAAIGLLAGLILALALERFDTKIRTRQAAEQHFG
                     YPVLAEIPVFRRKDRKGLPVASRPAGAAADAFRLLTVGVAAGPPAGGDGHPRERTLER
                     SPVPKTVLVTSAGPADGKSTVAANLAAAFGEQGKRVMVLSCDMRRPSIHRRFGLPVGP
                     GLSDAIVSANGQLELGPWKTRVRNVLVVPSGLVDENPGELLGSRRMHAALEEAASEAD
                     VVILDSSPVLVANDVTSLLAQVDSVVLVARANKTRADLAERTGEILRRAGAPVVGLAF
                     NGTREINLPTSYRRYYKHARGPVRL"
     gene            1174..1533
                     /locus_tag="VE646_06930"
     CDS             1174..1533
                     /locus_tag="VE646_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79758.1"
                     /translation="MLSSPEEIVRALVTYTDWWQPTTASIFRVGGRRNGSSSDGLHAG
                     LLETLSEREELCRRMQQIDPRDRHLLYLWYLKQLPAHEIAHEIRISRRQCFRRRAAAV
                     RELIELGDPDRARTVEN"
     gene            1573..2880
                     /locus_tag="VE646_06935"
     CDS             1573..2880
                     /locus_tag="VE646_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HZD79759.1"
                     /translation="MRSTADLRRAILSRDSCVAVIGQGYVGLSLACAAAEAGFRVTGV
                     DVDASRVEHLSRGRAAVAGVEQAALSAAVATGRLSFTTSARVVRDCRLVLVCVPTPLR
                     EGMPDLSFVDQACQDVAEHLAPGSLVVLESTTYPGTTEERVRPILERPGLVAGHDFLL
                     AYSPERIDPGGSEFSFRRVPRVVGGVNPEATGMAVLFYEQLVDKVMSVSSARAAELAK
                     LLENTFRHVNIALVNEMAMLCHEVGIDVWEVIEAAATKPFGFMRFSPGPGVGGHCIPL
                     DPTYLAWQVRRDAGHQFRVLEEAEDINAQMPAWVAARIGEVLNDRGKPVKEAGILVLG
                     AAYKPDVGDVRESPSIRVMNHLYRRGARISYHDPYIDQIAMNGGRLHCTELTTQRVQR
                     SDLVALLTPHAAYDLDWLAEHAQVLFDARNAYRTPCPENVVRL"
     gene            2877..3194
                     /locus_tag="VE646_06940"
     CDS             2877..3194
                     /locus_tag="VE646_06940"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HZD79760.1"
                     /translation="MSGVVRFRRSPQALWRRVGAGYVVGTKEDAEVHELLGGAAAVWD
                     GLAIARSRSGLVAELGSIHGVEPEEIRDQVEEVLERLLELGVIEQVVEMGVELGEVVQ
                     IDA"
     gene            3187..>3809
                     /locus_tag="VE646_06945"
     CDS             3187..>3809
                     /locus_tag="VE646_06945"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZD79761.1"
                     /translation="MPEAALEAMRRIAAFGLPGTPTPEPFAPTEREWSSLLSRIQWER
                     MSGLAVESAADGWLNLTDGQASELLNLHRAAMAWSLGVERKLLVLAEAFEVEGIAFAV
                     LKGASVAHTAYAEPCLRSFADLDLLVRTQDYERACRLLGALGHVRQRPEPRPGWEARF
                     GKASVHKHPQDRIEVDLHRTLVLGPFGLWIDPEELLDRRVTFELGGRT"
BASE COUNT          534 a         1268 c         1437 g          570 t
ORIGIN      
        1 cttcgagcgg cagatcaaag ctctgcggaa gtcgaacaat cccaccgccc cacagcagat
       61 cgcctccctc cggggcgaga tcggctcccg gaccagtcag aagcagctgc ttcccggcct
      121 gatcgtgatc caggagccgc gggccgtccc ggtggtgacc accgggttcc agccgccgtc
      181 gagccgcacc acgcgcgtgc tgatcgcggc cgcgatcggg ctcctggccg gcctcatcct
      241 ggcgctcgcc ctggagcggt tcgacaccaa gatccggacc cggcaggcgg ccgagcagca
      301 cttcgggtac ccggtgctcg cggagatccc ggtcttccgc cggaaggatc gcaagggctt
      361 gcccgtggcg agccggccgg ccggcgcggc ggccgatgcc ttccggctgc tcacggtggg
      421 ggtggcggcc gggccccccg ccggcggcga cgggcacccc agggagcgca cgctcgagcg
      481 ctcgcccgtg ccgaagaccg tcctggtgac cagcgcgggg cccgccgacg gcaagagcac
      541 cgtggccgcg aacctggccg ccgcgttcgg cgagcagggc aaacgggtga tggtcctgtc
      601 ctgcgacatg cgacgaccct cgatccaccg ccgcttcggg ttgccggtgg gaccgggcct
      661 gagcgacgcg atcgtctccg ccaacggcca gctggagctg gggccctgga agaccagggt
      721 ccgcaacgtc ctggtggtcc ccagcggcct ggtggacgag aacccgggcg agctcttggg
      781 gtcccggcgg atgcatgcgg cgctggagga ggccgcgtcc gaggccgatg tggtgatcct
      841 ggactcgtcc cccgtgctgg tggccaacga cgtgacctcg ttgctggccc aggtggactc
      901 ggtggtactg gtggccaggg cgaacaagac ccgcgcggac ctggccgagc gcaccggcga
      961 gatcctgcgg cgcgcaggcg ccccggtggt ggggctggcg ttcaacggca cccgggagat
     1021 caacctcccc accagctacc gccgctatta caagcacgcc cggggaccgg tccggctgtg
     1081 aacgagggat ggggacgagg gtgaggggta ggggatttcc ccgtcttccg cgtcaccgcc
     1141 cgcgtgcata cgctctaacg tcaggagggg tgtatgctct cctcgcctga ggagatcgtc
     1201 cgggcgctgg tcacctacac ggactggtgg cagcccacga cggcttcgat cttccgggtg
     1261 ggtggccgtc gcaacggttc ctcctccgac gggctccacg cggggctgct ggagacgctc
     1321 tccgagcgcg aagagctgtg ccggcggatg cagcagatcg atcctcgcga tcgccacctg
     1381 ctctacctct ggtacctgaa gcagttgccc gcacacgaga tcgcccacga gatccggatc
     1441 agccgccgcc agtgcttccg ccgccgtgct gcagcggtcc gcgagctgat cgagctcggc
     1501 gatccggacc gagctcggac cgtggagaac tgagccttcc gtcgcccgcc agaccgtcaa
     1561 gggggagaag ccttgcgctc caccgcagat cttcgccgcg ccatcttgtc cagggactcg
     1621 tgcgtggcgg tgatcggcca gggctacgtt gggctgtcgc tggcctgtgc cgccgccgag
     1681 gccggctttc gggtgaccgg cgtggacgtg gacgcctcgc gtgtcgagca cctgtcgagg
     1741 ggtcgagcgg ccgtggccgg cgtggagcag gcggcgctct cggcggcggt cgcgaccggc
     1801 cgcctgtcgt tcaccacctc tgcccgggtc gtccgcgatt gccggctggt gttggtctgc
     1861 gtgcccacgc cgctgcgcga gggcatgccg gacctgtcct tcgtggatca ggcatgtcag
     1921 gacgtggccg agcacctggc gcccgggtcg ctggtcgtgc tggagtccac gacctacccg
     1981 gggaccaccg aggagcgggt gcgtcccatc ctggagcggc cggggctggt ggcggggcac
     2041 gacttcctgc tggcctactc gccggagcgg atcgatccgg gcggcagcga gttctccttc
     2101 cggcgggtgc cccgggtggt gggcggggtc aaccccgagg ccactgggat ggcggtcctg
     2161 ttctacgagc agctggtgga caaggtgatg agcgtgagct cggctcgcgc cgccgagctg
     2221 gccaagctgc tggagaacac gttccggcac gtgaacatcg ccctggtcaa cgagatggcc
     2281 atgctctgcc acgaggtggg gatcgacgtg tgggaggtga tcgaggcggc ggccaccaag
     2341 ccgttcgggt tcatgcggtt ctcgcccggg ccgggcgtgg gcgggcactg catcccgctc
     2401 gatcccacgt acctggcgtg gcaggtgcgc cgcgacgccg gccaccagtt ccgggtgctg
     2461 gaggaggcgg aggacatcaa cgcgcagatg ccggcgtggg tggccgccag gatcggcgag
     2521 gtcctgaacg accgcggcaa gccggtgaag gaggcgggga tcctggtgct gggggccgcc
     2581 tacaagcccg acgtgggcga cgtccgcgag tccccgtcga tccgggtgat gaaccacctg
     2641 taccggcggg gggcgcggat ctcctaccac gatccgtaca tcgatcagat cgcgatgaac
     2701 ggcggccgct tgcactgcac cgaactgacc acccagcggg tccagcgctc ggacctggtg
     2761 gcgctgctga ccccccatgc cgcgtacgac ctggactggc tggccgagca cgcccaggtc
     2821 ctgttcgacg cccgcaacgc ctaccggacg ccgtgcccgg agaacgtggt gcgcctgtga
     2881 gcggcgtggt gcgcttccgg cgatcgccgc aggcactgtg gaggagggtg ggtgccggct
     2941 acgtggtcgg caccaaggag gacgccgagg tgcacgagct cctgggcggc gcggcggcgg
     3001 tctgggacgg gctggccatc gcccgaagcc ggagcgggtt ggtggcggag ctgggatcga
     3061 tacacggggt ggagcccgag gagatccggg accaggtgga ggaggtgctc gaacggctcc
     3121 tcgagctggg agtgatcgag caggtggtcg agatgggcgt ggaactcggc gaggtggtgc
     3181 agatcgatgc ctgaggccgc tctcgaggcg atgcgccgga tcgcggcgtt cggcctgccg
     3241 ggaaccccca caccggagcc gttcgcgccg acggagcgag agtggtcctc gctcttgtcg
     3301 cggatccagt gggagcggat gagcggcctg gccgtcgaga gcgccgcgga cggctggctg
     3361 aacctcaccg acgggcaggc ctcggagctc ttgaaccttc atcgcgcggc catggcctgg
     3421 agcctggggg tcgagcggaa gctgctcgtc ctggccgagg cgttcgaggt cgagggcatc
     3481 gccttcgcgg tgctgaaggg cgcgtcggtg gcccacaccg cctacgcgga gccgtgtcta
     3541 cggtccttcg ccgacctgga cctgctggtg cggacccagg actacgagcg cgcctgccgg
     3601 ctcctcggcg cgctggggca cgtgcgccag cggcccgaac cgcggcccgg gtgggaggcc
     3661 cggttcggca aggccagcgt ccacaagcac ccgcaggacc ggatcgaggt cgacctgcac
     3721 cgaacgctgg tgctgggccc gttcgggttg tggatcgacc cagaggagct gttggaccgg
     3781 cgggtgacgt tcgagctcgg cggtcggac
//
LOCUS       DASQQQ010000174         2667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_397421, whole genome shotgun sequence.
ACCESSION   DASQQQ010000174 DASQQQ010000000
VERSION     DASQQQ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397421"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..2587
                     /locus_tag="VE646_06950"
     CDS             176..2587
                     /locus_tag="VE646_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZD79762.1"
                     /translation="MAAVWFADIVGYGRLASRNENEALEIVRLFQRTCRDVVRRYDGR
                     VVKFTGDGALAEFHSTESAVRAACGLESAVRARAEAGGLTQPQLHIGLHVGEIASAPD
                     GDLYGEGLNLASRLEGLAGPGQVLVSEDVRRQLHQRPEFRFIPLGEREIPDSDEPIAV
                     YCVVERSGAAALPVLPDVAPGPWRSLHRELARRGVYRAAVIHLIVAGVAMAAAVAVLD
                     RAQAPSWAARAVALLAVLGFPVVLWLAWTYEIGRGGLRRHEPGTPDEAPARPLLAFVG
                     LILVVALIAGAIVFVQPPLRGELPELPMNRIAVLYFDDLSPDGRLRHLVDGLTESLIH
                     DLSGVPGIEVVSRNGVKPFENASVPPDSVARALGAGTLVQGSVDESDGRLRVRVQLID
                     GRKGTVLESESLERAHGDLFALQDELTERVGGFLRRQLGAEIRLVEMRAETDNVRAWE
                     LVHQAEGLKDEAQPLVQAGALAEAGRLFERADSLLIEAHHVDSLWVQPVVQRGWLAYE
                     RSRWTENSDMTASLRWNEEAARIAEAALRLGPGDPEALNLRGSLRVWRAIQAPERDPA
                     EADAQLDRAIEDLRGSIEADPRQAGAWSRLSWGLAARGDRPGAREAARRAYEADAYLD
                     EANEIIWRGFSTSYDLEEADEAERWCEEGRRRFPADATFVSCHIWLLTMPGASPPPDS
                     VWALLDAYDRLVPPHAVGRRRTVRMGVAAALARAGLADSALAVATRARAGDSDLAGES
                     AYFEAFVRTLVGQEESATRLLADYLRASPAQRHEVAVTWWFEPLHDRPDFQALVGETR
                     QPGGP"
BASE COUNT          362 a          933 c          972 g          400 t
ORIGIN      
        1 ggagagcggg ccccttcccg gaaggatccg ggcgggggcc cgttcttgtt gtgagttccg
       61 gggccggtcc accggccctc ttgcaggcgg atgacgctac cgtatactgg ttaacgccag
      121 gccaggccca cccggagagg acgtcatgcc ccagcggggg accctgccac acaggttggc
      181 ggcggtttgg ttcgccgaca tcgtcggcta cggccggctc gcgtcccgca acgagaacga
      241 agcgctcgag atcgtccggc tgttccagcg aacgtgccgt gacgtcgtgc gccggtacga
      301 cggccgcgtc gtcaagttca ccggcgacgg ggccctggcc gaattccaca gcaccgagtc
      361 cgccgtacgg gcggcctgtg ggctcgagtc ggccgtgcga gcccgggccg aagccggcgg
      421 gctcacccag ccccagctcc acataggtct ccacgtcggc gagatcgcca gcgcccccga
      481 cggtgacctc tatggagaag ggctgaacct ggcttcgcgg ctcgagggcc tggccgggcc
      541 cggacaggtc ctcgtcagcg aggacgtgcg ccgtcagctc caccagcgcc ccgagtttcg
      601 cttcatcccc ctgggtgagc gcgagatccc ggactcggac gaacccatcg ccgtgtactg
      661 cgtggtggag cgctcgggcg ccgcagcgct cccggtgtta ccggacgtcg cgccgggtcc
      721 ctggcgatcg ctgcaccggg agctcgctcg tcggggggtc tacagggccg ccgtgatcca
      781 cctgatcgtg gccggcgttg ccatggccgc ggcggtcgcc gttctggatc gagcgcaagc
      841 gccgagttgg gccgctcgag ccgtggcgct gctggcggtc ctcgggttcc cggtcgtgct
      901 gtggctggcc tggacgtacg agatcggccg cggcgggctg aggcgccacg agccggggac
      961 cccggacgag gcgccagcgc gcccgctgct cgcgttcgtt ggcttgatcc tggtcgtggc
     1021 cctgatcgcc ggtgccatcg tcttcgtcca gccgccgctc cggggcgagc tccccgagct
     1081 gccgatgaac cggatcgcgg tgctctactt cgacgacctc agcccggacg gccgtttgcg
     1141 gcacctggtg gacgggctca ccgaatctct catccacgat ctctccgggg tccccggtat
     1201 cgaggtcgta tcgcggaacg gcgtgaagcc gttcgagaac gcgagcgttc cgccggacag
     1261 cgtggcccgg gccctcgggg cgggaacgct cgttcagggc agcgtggacg agtccgatgg
     1321 gcgtcttcga gtccgggttc agctcatcga tggtcgcaaa ggcacggttc tcgagagcga
     1381 gtctctggag cgggcccacg gggacctgtt cgccctgcag gatgagctga cggaacgggt
     1441 gggaggcttc ctgcgccgcc agctgggcgc ggagatccgt ctcgtcgaga tgcgcgccga
     1501 gaccgacaac gtgcgcgcct gggagctcgt ccatcaggcc gagggcctga aggatgaggc
     1561 ccagccgctg gtccaggccg gggccctcgc cgaagcgggc cgtctcttcg agcgggccga
     1621 ttcgctgctc atcgaggccc accacgtgga ctccctgtgg gtccagcccg tcgttcaacg
     1681 cggatggctg gcctacgagc gttcgcgctg gacggagaac agcgacatga cggcctccct
     1741 gcgctggaac gaggaggccg cccggattgc cgaggccgct ctccggcttg gccccggtga
     1801 tccggaggcc ctcaacctgc gcgggagcct gcgcgtgtgg cgggccatcc aggccccgga
     1861 gcgcgacccc gccgaggccg atgcccagct cgatcgagcc atcgaggacc tccgaggctc
     1921 gatcgaggcc gacccccgcc aggcaggggc ctggtcacgg ctgagctggg ggctggcggc
     1981 gcgcggcgac cggccggggg cccgggaggc cgcccgacgg gcctacgaag ccgacgccta
     2041 tctggacgaa gcgaacgaga tcatctggcg tggtttctcg acatcgtacg acctcgagga
     2101 ggccgacgag gcggagcgct ggtgcgagga ggggagacgg cggttcccgg ccgacgcgac
     2161 cttcgtttcg tgccatatct ggctgctgac catgccgggg gcctcccccc cgccggacag
     2221 cgtgtgggcc ctgctcgacg cctacgaccg ccttgtcccg ccccacgcgg tgggtcgtcg
     2281 ccgaacggtg cgcatgggcg tcgccgcggc tctggcccgg gctggactgg ccgacagcgc
     2341 gctcgcagtg gccacccggg cgcgagcggg agacagcgac ctggccggag agagcgccta
     2401 tttcgaggcc ttcgtgcgaa cgctcgtcgg tcaggaggag tcggccacga ggctcctcgc
     2461 ggattacctg cgggccagcc cggctcagcg ccacgaggtc gcggtcacct ggtggttcga
     2521 gccgcttcac gaccgaccgg acttccaggc gctggtcggc gagacccgtc agcccggcgg
     2581 accctgaccg gcccccccgg ccacagctga caccgcctct actcggcggt cgcggtcccg
     2641 gctgaatgct gcacagccgc gaaggga
//
LOCUS       DASQQQ010000175         4259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_286539, whole genome shotgun sequence.
ACCESSION   DASQQQ010000175 DASQQQ010000000
VERSION     DASQQQ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286539"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VE646_06955"
     CDS             complement(<1..451)
                     /locus_tag="VE646_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZD79763.1"
                     /translation="MRVTVVGSGFVGQTTAMRLVQRGLAEVVLIDIIEGLPQGLALDI
                     RQSAPIVGFEPHVIGTNDYADTAGSDVVVITAGFPRQPGMSRMDLLGKNAGVVREVVE
                     KVAPGSPEAILIVVTNPLDEMTYLAAEVSGFPKERVVGMAGVLDSGRL"
     gene            complement(509..1414)
                     /locus_tag="VE646_06960"
     CDS             complement(509..1414)
                     /locus_tag="VE646_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZD79764.1"
                     /translation="MSRRPLIETAHGTRREAFGPQEWALLVAIAAMWGSSFLLIEIGL
                     EGFGPGLVGFGRVAVGALTLSLLPGSRRPVERADLPRVALLGTVWMAAPLVLFPIGQR
                     WIDSSLAGMINGAVPIFAALTAAVLLRRPPPARQLVGVSVGFVGVVGVLWPAARGADA
                     TALGAALVLLAVLCYGVAVNLAVPLQQRYGALPVLFRAQLVALVLLAPAAVISLPDSD
                     LLWTSALAVGALGFFGTGWAFVAMTTLVGRVGATRGSVSIYLLPVVAIALGVVFRDET
                     VAAISLIGTALVLLGAYLTSRREGP"
     gene            complement(1449..2534)
                     /locus_tag="VE646_06965"
     CDS             complement(1449..2534)
                     /locus_tag="VE646_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HZD79765.1"
                     /translation="MGTHRITLIPGDGVGPELVEAARRTVEATGVRVRWDVQEAGAEA
                     AEREGAPLPDRVLASIRANGVALKGPLTTPVDGPHRSANVALRVALDLYAGVRPCRSF
                     PGVRSRYEGVDVVVIRENTEGSYTGIEFEQGGPNAAELIAFIERVTGRPVRQDSGLSI
                     KAISMGASTRIARFAFAYARAHGRRAVTAGHKANIMKLSDGVFLEAARQVAGEHPEIG
                     FEDRIIDNLAMQLARRPQAFDVLVLPNLYGDIVSDLCAGLIGGLGLAPGADLGDDCAV
                     FEAQHGSAPRYRGTGRANPMALMLSGAMMLRHLGEREAGDRLEGAVATVIARGQVTAD
                     LKPIRDDPSAATTAEVREAVIGELISR"
     gene            2653..3771
                     /locus_tag="VE646_06970"
     CDS             2653..3771
                     /locus_tag="VE646_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HZD79766.1"
                     /translation="MTEKGLREIVAAQTRLSDIDGEAGRLWYVGYAIEDLAANASYEE
                     TVHLLHELRLPTQAELDELTAFLVEQREPSRFIVDLMPTLAQQTSPMSMLRTSVSAAS
                     AYDPDGWDNSPEANRRKALRLISRTPTFLAMYHRLRTGQEIVPPDPKLSQAANFLYML
                     TGLEPSPEDADVLDTTFTLYADHTMNASTFTARIIASTLADMHSAISGAIAALKGPLH
                     GGANEEAMKMLEEVADPANAELYVHDRLARKEKIMGFGHAVYRTMDPRARILKRLSHD
                     VGERHGDLGWYRISEAIEATTRLQKQLFPNVDFYAASVYHMLGIPTDLMTPIFAIARM
                     AGWTAHVREQYEDNRIIRPDSEYVGPRDQRWVPIPERG"
     gene            complement(3796..>4259)
                     /locus_tag="VE646_06975"
     CDS             complement(3796..>4259)
                     /locus_tag="VE646_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79767.1"
                     /translation="VRRLALASIARLLDGVRPQNKAAATALTDELMAGLLRYTGVLLT
                     IALAVIAVALVTGPYPWAVRLRGWAVDLGRAVAAAPGAARRQPVAVWIRAHRDTLVFG
                     GAAAGVASLFFFDLSLLGFLVVVLLLALFEVAVLRVAASAGVPVASEGGDP"
BASE COUNT          697 a         1608 c         1435 g          519 t
ORIGIN      
        1 gcaggcggcc cgagtcgagc acgcccgcca tccccaccac ccgctccttc gggaacccgg
       61 agacctccgc ggccaggtag gtcatctcgt cgagcgggtt cgtgaccacg atcaggatcg
      121 cctcgggcga gccgggcgcc accttctcca ccacctcgcg gacgaccccc gcgttcttcc
      181 ccaacaggtc catccggctc atccccggct gccgcgggaa ccccgcggtg atcaccacga
      241 cgtcggagcc ggccgtgtcc gcgtagtcgt tggtcccgat gacgtgcggc tcgaacccca
      301 cgatcggcgc cgactgacgg atgtctagcg cgagcccctg ggggagccct tcgatgatgt
      361 cgatcaggac gacctcggcc aggccccgct ggacgagccg catggccgtg gtctgcccga
      421 cgaacccgct tccgacgacg gtgacgcgca tggtcgatgc tcctcggtcc ggatgcggcc
      481 catcctactc accgacctcg aagacctgtc acggcccctc tcgacggctc gtcaggtagg
      541 cgcccaggag gaccagggcc gttccgatca gcgagatcgc ggccaccgtc tcgtcccgga
      601 agacgacgcc gagggcgatg gccaccaccg gcaggaggta gatcgagacc gatccccggg
      661 tggctcccac ccggcccacg agcgtcgtca tggccacgaa cgcccagccg gtgccgaaga
      721 acccgagggc tcccaccgcg agcgcgctgg tccacaggag atccgagtcc gggagcgaga
      781 tgaccgccgc cggcgcgagg agcaccagcg ccaccagctg ggcccggaac aggaccggga
      841 gcgccccgta gcgctgctgg agcggcaccg cgaggttgac ggccacgccg tagcagagca
      901 cggccagcag gaccagggca gcaccgagcg cggtggcgtc tgcgccccgg gccgccggcc
      961 acaggacccc caccaccccg acgaagccaa ccgagacccc gaccagctgg cgagcgggcg
     1021 gcgggcgccg cagcaacacc gcagccgtga gcgccgcgaa gatcggcacc gccccgttga
     1081 tcatcccggc cagcgaggaa tcgatccacc gctgcccgat ggggaacagc accaaggggg
     1141 ccgccatcca gacggtcccg agcagcgcca cgcgaggcag gtcggcccgt tcgaccggcc
     1201 ggcgcgaccc cggcagcagc gagagcgtga gcgcgccgac cgcgacgcga ccgaacccca
     1261 ccaggccggg gccgaagccc tcgagtccga tctcgatgag caggaacgac gaaccccaca
     1321 tggccgcgat ggccacgagc agcgcccact cctgcgggcc gaacgcctcg cggcgcgtgc
     1381 cgtgggccgt ctcgatcagc ggacgtcggc tcatctgggc catcggaccg ccaacgcgtg
     1441 gggatccctc accgtgagat cagctccccg atcacggcct cccggacctc ggccgtggtg
     1501 gcggcgctcg ggtcgtcccg gatgggcttc aggtcggcgg tcacctggcc ccgcgcgatc
     1561 acggtggcga ccgccccctc caggcgatcg ccggcctccc gctcacccag gtgccggagc
     1621 atcatcgccc cggagagcat cagcgccatc gggttcgccc gcccggtccc gcggtaccgg
     1681 ggggcgctcc cgtgctgcgc ctcgaagacc gcgcagtcgt cccccaggtc cgcgccgggg
     1741 gccagaccca ggccgccgat cagtcccgcg cacaggtccg agacgatgtc cccgtaaagg
     1801 ttcggcagga ccaggacgtc gaacgcctgg ggccggcggg ccagctgcat cgcgaggttg
     1861 tcgatgatgc ggtcctcgaa gccgatctcc gggtgctcgc cggcgacctg ccgggcggcc
     1921 tccaggaaca ccccgtcgga gagcttcatg atgttggcct tgtgtccggc ggtgaccgcc
     1981 cgccggccgt gcgcccgggc gtaggcgaag gcgaaccggg cgatccgcgt gctcgcgccc
     2041 atcgagatgg ccttgatcga cagcccggag tcctgtcgga cgggtcggcc ggtcacccgc
     2101 tcgatgaacg cgatgagctc ggccgcgttc gggcccccct gctcgaactc gatccccgtg
     2161 tagctgccct ccgtgttctc gcggatcacg accacgtcga ccccctcgta gcgggagcgg
     2221 acgccgggga acgaccggca cgggcggacc cccgcgtaca ggtccagcgc cacccgcagc
     2281 gccacgttcg cgctgcgatg gggtccgtcg accggagtgg tcagggggcc cttgagcgcc
     2341 acaccgttgg cgcggatcga cgcgagcacc cggtcgggca acggcgcgcc ttcccgttcg
     2401 gccgcctcgg ctcccgcctc ctgcacgtcc caccggaccc ggacgccggt ggcctcgacg
     2461 gtccggcggg ccgcctccac gagctcgggg ccgaccccgt cgcccgggat cagggtgatg
     2521 cggtgcgtgc ccacgcgctc ccttctgcga tgatgcggct gcggctggac ccggacctat
     2581 cacgaggtcg agataccgtg attgtggtgg aatcaaggtc cggatgacat ccgaacccga
     2641 tgggagggtg gggtgaccga gaagggcctg cgggagatcg tcgcggcaca gacccggctg
     2701 tccgacatcg acggggaagc cgggcgcctg tggtacgtgg ggtacgcgat cgaggacctg
     2761 gccgcgaacg ccagctacga ggagaccgtg cacctgttgc acgagctccg cctgccgacc
     2821 caggcggagc tggacgagct cacggcgttc ctggtcgagc agcgggagcc ctcccggttc
     2881 atcgtcgacc tgatgcccac gctcgcccag cagacctcgc ccatgtcgat gcttcgcacg
     2941 agcgtgtccg cggcctccgc gtacgacccc gatgggtggg acaactcgcc cgaggccaac
     3001 cgacgcaagg ccctccggct gatctcccgg accccgacct tcctcgccat gtaccaccgc
     3061 ctgcggacgg ggcaggagat cgtgcccccg gatccgaagc tgtcgcaggc cgcgaacttc
     3121 ctctacatgc tcaccggcct cgagccctcg ccggaggacg ccgacgtcct ggacaccacg
     3181 ttcacgctct acgcagatca cacgatgaac gcgtccacgt tcacggccag gatcatcgcc
     3241 tccacgctcg ccgacatgca ctcggcgatc agcggagcga tcgccgcgct gaaggggccc
     3301 ctgcacggcg gcgcgaacga ggaggccatg aagatgctcg aggaggtggc cgaccccgcc
     3361 aacgcagagc tgtacgtcca cgatcgcctc gcgcgcaagg agaagatcat gggcttcggc
     3421 cacgccgtct accggaccat ggacccacgg gccaggatct tgaagcgcct gtcccatgac
     3481 gtcggcgagc gacacggcga cctcggctgg taccggatct ccgaggccat cgaggcgacc
     3541 acgcgcctcc agaagcagct gttcccgaac gtggacttct acgcggcaag cgtctaccac
     3601 atgctgggga tcccgaccga cctcatgacc ccgatcttcg cgatcgcccg catggccggc
     3661 tggaccgccc acgtccgaga gcaatacgag gacaaccgga tcatccggcc cgattccgag
     3721 tacgtgggac cgcgcgacca gcgctgggtg ccgatccccg agcgcggctg agcgcggggg
     3781 tcgctcggca gccgatcacg ggtcgccccc ctccgaggcg accgggaccc cagcggaggc
     3841 cgccacccgg agcacggcga cctcgaagag cgcgagcagc aacaccacga ccaggaaccc
     3901 gaggagggag aggtcgaaga agaacaggga ggcgacgccg gcggccgccc ccccgaacac
     3961 cagggtgtcc cggtgggctc ggatccagac ggccaccggc tgccggcgag ccgctcccgg
     4021 tgcggcggcc accgcgcggc ccagatcgac cgcccagccc cgcagacgca cggcccatgg
     4081 gtacggcccg gtgaccaacg ccaccgcgat cacggccagc gcgatcgtga gcaggacgcc
     4141 ggtgtaccgg agcagaccgg ccatcaactc gtcggtgagg gcggtcgctg ccgccttgtt
     4201 ctgcggcctc accccgtcga gcagccgagc gatcgaggcc aacgccagcc ggcgcacca
//
LOCUS       DASQQQ010000176         4035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_368432, whole genome shotgun sequence.
ACCESSION   DASQQQ010000176 DASQQQ010000000
VERSION     DASQQQ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368432"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2178)
                     /locus_tag="VE646_06980"
     CDS             complement(<1..2178)
                     /locus_tag="VE646_06980"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HZD79768.1"
                     /translation="MPDALAPVRDRVSFAKLDEVLPLPDLVGIQRESFDWLLAEGLKE
                     VLEEVSPIEDFTEQFQLHFGKHTFKEQKFSEEECKDKDQTFSAPLFVEAMFVNKATGE
                     IKEQEVFMGDFPMMTEKGTFIINGTERVVVSQLVRSPGAYFSREQDKTTDKDIYIAKV
                     IPSRGAWLEFDVDKKDTVGVRIDRKRRQNVTVLLKALGWSTEEILDLFEGARSVQNTL
                     EKDHFETPEEALEDIYRKLRPGEPPTAESARTLLENLFFNHKRYDLARVGRYKVSKKL
                     EPAEGALLKQLRARAKQLAELDNPDKKAWDQPRFRVWGELQKDEHGDGAPKYKGILSY
                     EDMLKTVRYLVKLHAGEEGYEPDDIDHFGNRRLRSVGELIQNQIRIGLSRMERVVRER
                     MTTQDVEAITPQTLINIRPVVASIKEFFGSSQLSQFMDQTNPLAGLTHKRRLNALGPG
                     GLSRERAGFEVRDVHPSHYGRMCPIETPEGPNIGLIGSLSTYGRINEFGFVETPYRRV
                     VKGRVTDKIDYLSADEEDKHVIAQANAALNEDSTFQNADVLVRRKGGEVDQVPPEEID
                     YMDVSPKQIISVAAALIPFLEHDDANRALMGANMQRQAVPLLRSEAPLVGTGVEFRAA
                     VDAGDVVLAEEAGVVEDVSADEVVVRERGGTLRTYRLQKFRRSNQGTSMNQKPLVFEG
                     DKVSRRQVIADGPSTESGELALGRNLVVAYMPWEGHNYEDAIII"
     gene            complement(2384..3058)
                     /locus_tag="VE646_06985"
     CDS             complement(2384..3058)
                     /locus_tag="VE646_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79769.1"
                     /translation="MSAFNTPGHRFEALRDSRSTPRPTASFTHILSGSFPTTPPRPAT
                     PDLRYRRFALDQPVEASFLRFVAEHPQSDTAGAVQVAEVQAFGSGDVVVQEQSGGGVD
                     PVHDEGLALVPTADAQLTLNLMLEGACLFPPPTQGVDAWVTELPDGYQDGTSVISVRP
                     ESDLPQARADVDVFFLSSDCQLTGSVATASAGESGTVPQGTWYVVTELYTSVASRIVL
                     DAGRVG"
     gene            3045..>4035
                     /locus_tag="VE646_06990"
     CDS             3045..>4035
                     /locus_tag="VE646_06990"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HZD79770.1"
                     /translation="MNALTWSSAQTAFDRARASSPERTQAQTEIDVLTSRIANYENQI
                     SDLSLIDLDPGEVIQPADLPTGPASPNHLLNGGLALFVGLALGIGFAFLRERLDDRLA
                     GRDEFEEAIGAPTLAVIPRVTGWRKRERAELPALKAPKGAPAEAYRTVRTNLLFMARD
                     GDIKTIEITSPATAEGKTTTVANLAVSLAQAEKRVIAVSCDLRKPRLHRFFGLTNEVG
                     LTSVLAGTATLTDAILRPFGVDTLRVMSSGPIPNNPSELLSSDRMEALLAQLRTLADF
                     VVIDTPPTLVVSDGLILAPKTDAVLMVADASTTSHGAARHLREQLEQVGGNIVG"
BASE COUNT          588 a         1416 c         1375 g          656 t
ORIGIN      
        1 gatgatgatg gcgtcctcgt agttgtggcc ctcccagggc atgtaggcga ccaccaggtt
       61 gcgccccagg gcaagctcgc cggactcggt ggacggtccg tcggcgatga cctgccggcg
      121 cgagaccttg tcgccctcga acaccagggg cttctgattc atggacgtgc cctggttgga
      181 ccggcggaac ttctggagcc ggtaggtccg cagggtcccg ccgcgctcgc ggaccacgac
      241 ctcgtcggcc gagacgtcct cgaccacccc ggcctcctcg gccagcacca catcgccggc
      301 gtccaccgcg gcccggaact cgacccccgt gcccaccagc ggggcctcgg agcgcagcag
      361 cgggaccgcc tggcgctgca tgttggcgcc catcagggcc cggttggcgt cgtcgtgttc
      421 caggaacggg atcagggccg ccgccaccga gatgatctgc ttgggcgaca cgtccatgta
      481 gtcgatctcc tccggcggca cctgatcgac ctcgccgccc ttgcgccgga ccaggacgtc
      541 ggcgttctgg aacgtgctgt cctcgttgag cgccgcgttg gcctgggcga tgacgtgctt
      601 gtcctcctcg tcggcggaca ggtagtcgat cttgtcggtg accctgccct tgaccacccg
      661 gcggtacggg gtctcgacga acccgaactc gttgatccgc ccgtaggtgg acagggaccc
      721 gatcaggccg atgttcgggc cctccggggt ctcgatgggg cacatccggc cgtagtggct
      781 gggatgcacg tcccggacct cgaacccggc gcgctcgcgc gacaggcccc ccgggccgag
      841 ggcgttcagc cgacgcttgt gcgtgagccc ggccagcgga ttcgtctggt ccatgaactg
      901 gctgagctgg ctcgagccga agaactcctt gatcgaggcc acgacgggcc ggatgttgat
      961 gagggtctgg ggcgtgatcg cctcgacgtc ctgggtggtc atgcgctcgc ggaccacccg
     1021 ctccatccgg gacagcccga tccggatctg gttctggatc agctcgccca cgctgcgcag
     1081 gcgccggttg ccgaagtggt cgatgtcgtc gggctcgtag ccctcctccc cggcgtggag
     1141 cttgaccagg taccggacgg tcttgagcat gtcctcgtag gacaggatcc ccttgtactt
     1201 gggggcgccg tcgccgtgct cgtctttctg gagctcgccc cacacgcgga accgcggctg
     1261 gtcccaggcc ttcttgtccg ggttgtccag ctccgcgagc tgcttggccc gggcccggag
     1321 ctgcttgagc agcgcgccct cggcgggctc gagcttcttg ctcaccttgt agcgacccac
     1381 ccgggccagg tcgtagcgct tgtggttgaa gaacaggttc tccagcagcg tgcgggcgga
     1441 ctcggccgtg ggcggctcgc ccgggcgcag cttgcggtag atgtcctcca gggcctcctc
     1501 gggcgtctcg aagtggtcct tctccagggt gttctggacg gaccgggcgc cctcgaacag
     1561 gtccaggatc tcctcggtgc tccagcccag ggccttcagc aggaccgtga cgttctggcg
     1621 gcgcttgcga tcgatccgga cgccgaccgt gtccttcttg tcgacgtcga actccagcca
     1681 ggcgccccgg ctggggatga ccttggcgat gtagatgtcc ttgtccgtgg tcttgtcctg
     1741 ctcacggctg aagtaggccc cgggcgagcg gaccagctgg gagaccacga cccgctcggt
     1801 gccgttgatg atgaaggtcc ccttctcggt catcatgggg aagtcgccca tgaagacctc
     1861 ctgctccttg atctcgccgg tggccttgtt gacgaacatg gcctcgacga acagcggggc
     1921 gctgaaggtc tgatccttgt ccttgcactc ctcctcggag aacttctgct ccttgaaggt
     1981 gtgcttgccg aaatgcagtt ggaactgttc ggtgaagtcc tcgatgggag agacttcctc
     2041 caggacctcc ttcaggccct cggccaggag ccagtcgaag gactccctct ggatgcccac
     2101 gaggtcgggg agcggtagga cctcgtcgag cttggcgaaa ctgacacgat ctcgaacagg
     2161 cgccagagcg tcaggcaaga actatccccc ctagggtgaa agacacgcct ggaatcaggg
     2221 catgcgggat gcggcacggc aaacgagtag tttacgtgcg caggcgcgca ccgtcaaccc
     2281 ccgggatggg gtcccggtcg aaccggccgc aatgtactcc tcccggagcc ccaggtcaac
     2341 cgccggatgg gccccatccg gcggttgacc tggggccatc cggctacccc acgcgccccg
     2401 cgtccagcac gatccggctc gcgacgctcg tgtagagctc ggtcaccacg taccacgtgc
     2461 cctgggggac ggtgccgctc tcccccgcgc tggcggtggc gacgctgccg gtgagctggc
     2521 agtccgagga caggaagaac acgtccacgt cggccctggc ctgcggcagg tcgctctcgg
     2581 gccgcaccga gatcacgctg gtcccgtcct ggtagccgtc cggcagctcg gtgacccagg
     2641 cgtcgacgcc ctgggtgggc ggcgggaaca ggcacgcgcc ctccagcatc aggttcagcg
     2701 tgagctgggc gtccgcggtg ggcaccagcg ccaacccctc gtcgtggacc gggtccacgc
     2761 ccccgcccga ctgctcctgg accaccacgt cgcccgagcc gaacgcctgg acctcggcga
     2821 cctgcacggc gccggccgtg tcgctctgcg ggtgctcggc cacgaaccgc aggaacgagg
     2881 cctccaccgg ctggtcgagc gcgaaccgcc ggtaccgcag gtccggggtc gccggccgag
     2941 gcggggtggt ggggaacgag ccggacagga tgtgcgtgaa gctcgccgtc ggtcgaggcg
     3001 tagaccgaga gtcccgcagc gcctcgaacc ggtggcccgg ggtgttgaac gcgctcacct
     3061 ggagctcggc gcagaccgcg ttcgaccggg ccagggcgag ctcgccggag cgcacccagg
     3121 cgcagaccga gatcgacgtg ctgacctcgc ggatcgcgaa ctacgagaac cagatcagcg
     3181 acctcagcct gatcgacctc gatcccggcg aggtgatcca gccggccgac ctgccgaccg
     3241 gccccgcgtc gccgaaccac ctcctcaacg gcgggctggc gctgttcgtc gggctggccc
     3301 tcgggatcgg gttcgcgttc cttcgggagc ggctggacga ccggctggcc ggccgcgacg
     3361 agttcgagga ggcgatcggg gcgccgaccc tggccgtgat cccgcgcgtc accggctggc
     3421 gcaagcgcga gcgcgccgag ctcccggcgc tgaaggcccc gaagggcgcc ccggccgagg
     3481 cctaccgcac cgtccgcacc aacctgctgt tcatggcgag ggacggcgat atcaagacca
     3541 tcgagatcac gagcccggcg acggccgagg gcaagaccac caccgtggcg aacctcgccg
     3601 tgtccctggc gcaggccgag aagcgggtga tcgcggtctc ctgcgacctc cgcaagccgc
     3661 ggctgcaccg gttcttcggg ctgaccaacg aggttggcct cacgagcgtg ctcgccggca
     3721 ccgccacgct caccgacgcg atcctgcggc cgttcggcgt cgacacgctc cgcgtgatgt
     3781 cgagcggccc gatcccgaac aacccctcgg agctcctgtc gtcggaccgg atggaggcgc
     3841 tgctcgccca gctccgcacg ctggccgact tcgtggtgat cgacacgccg cccacgctgg
     3901 tggtctccga cggcctgatc ctggccccga agaccgatgc ggtgctcatg gtggccgacg
     3961 cctcgaccac ctcacacggg gcggcgcgtc acctccgcga gcagctcgag caggtcgggg
     4021 gcaacatcgt cggcg
//
LOCUS       DASQQQ010000177         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_91702, whole genome shotgun sequence.
ACCESSION   DASQQQ010000177 DASQQQ010000000
VERSION     DASQQQ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91702"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1110)
                     /locus_tag="VE646_06995"
     CDS             complement(106..1110)
                     /locus_tag="VE646_06995"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HZD79771.1"
                     /translation="MTSRHATIAGVGSALPERVVPNAWFESFLQTSDAWIRERTGIRE
                     RRFAGEDQATSDLALEATRRALEAADVTPEQVDLLVCATLTPDTPIPAAAVWVQRKLR
                     ITCPAFDVNAACAGFSYAVSTGTAFIESGTADTVVVIGAEVLSRVLDLTDRSTCVLFG
                     DGAGAVVLRRSERPGILGSVLGADGQAAEILIIPAGGSARPASSETLAERAHTIRMPS
                     GREVFKRAVVEMAAACRQLLEKSGYAPDDVDLLIPHQANARIMNAVAERLGIDADRAV
                     VDVAEVGNTSAASIPIALDRAWRAGRIRRDALVLITSFGAGLAWGANLVRWTAPSPTS
                     "
     gene            complement(1175..2101)
                     /locus_tag="VE646_07000"
     CDS             complement(1175..2101)
                     /locus_tag="VE646_07000"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZD79772.1"
                     /translation="MGLAILFPGQGSQYPAMADPWLEHEAGRSVLDVASEVLGRDIVD
                     ACRDEALLATTAFVQPALLACDVAAFRVLAAEGVEPAAAAGHSLGEFAALVAAGAMDL
                     PEALEAVRVRGAAMQDASERFPGTMVALIGLTEEQADQVCRASRGDDVLAVANENAAN
                     QFVLSGSEPAVARAESEARRLGGRAIRLNVAGAFHSPLMEPALQAIRAVLERTTIRPP
                     VFPVVANVTAGPEQDPNTIRELLGRHVIRPVRWHASMRTLAGMGVGALVEAGPGQVLS
                     RLARRSFPGITVATVGSAAEAAAARAEAEPRS"
     gene            2266..>2520
                     /locus_tag="VE646_07005"
     CDS             2266..>2520
                     /locus_tag="VE646_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HZD79773.1"
                     /translation="MAERVQRGLPASQRQRNAGAEGLFGLLGQDMAVDLGTANTLVYV
                     RRSGIVLNEPSVVAINTLTGAILAVGNEAKRMIGRTPSHIQ"
BASE COUNT          373 a          962 c          869 g          316 t
ORIGIN      
        1 cgtcgctggt gcggaacccg atcgccaccc tccacccgtc ggccgccagt gcctccgcgc
       61 acgcccggcc gatgccccga gctgcacccg tgaccagggc gaccttcacg acgtcgggct
      121 cggcgcggtc catcggacca ggttcgcccc ccaggccagc ccggcgccga acgacgtgat
      181 cagcaccagg gcgtcccgcc ggatccggcc cgcccgccac gcgcgatcca gggcgatggg
      241 gatcgaggcg gccgaggtgt tccccacctc ggccacgtcc accaccgcgc gatccgcatc
      301 gatccccagg cgctcggcca ccgcgttcat gatccgggcg ttggcttggt gggggatcag
      361 caggtcgacg tcgtccggcg cgtaccccga cttctccagc agctggcggc acgccgcggc
      421 catctcgaca accgcccgct tgaagacctc tcggcccgag ggcatgcgga tcgtgtgggc
      481 gcgttccgcc agggtctcgg acgatgccgg ccgggccgag cccccggcgg ggatgatgag
      541 gatctcggcc gcctgcccgt ccgctccaag cacggaaccg aggatgcccg gacgctcgga
      601 gcgtcgaagc acgacggccc cggctccgtc cccgaacagc acgcaggtcg aacggtcggt
      661 caggtcgagc acgcgcgaga ggacctccgc gccgatcacc accacggtgt ccgcggtccc
      721 ggactcgatg aaggcggtcc cggtcgacac cgcatacgag aatccggcac aggcggcgtt
      781 gacgtcgaac gccggacagg tgatgcgcag cttgcgctgc acccacaccg ccgcggccgg
      841 tatcggggtg tcgggagtga gggtggcgca cacgaggagg tccacctgct cgggcgtcac
      901 gtcggccgcc tccagcgcac gccgcgtggc ctccagcgcg aggtcggagg tggcctggtc
      961 ctcgccggcg aaccggcgct cgcggatccc cgtacgctcg cggatccagg catcgctggt
     1021 ctgcaggaac gattcgaacc acgcgttcgg caccacccgc tctggaaggg ccgaaccgac
     1081 gcccgcgatc gtcgcgtgcc gcgacgtcac cgggcccccc accgttggcg catggagccg
     1141 aggatagcgt cccggggagc cgaacgccgg cgggtcacga gcgcggctcc gcctcggccc
     1201 gggcggcggc ggcctccgcc gcagagccca ccgtcgcgac cgtgatgccg gggaacgaac
     1261 gtcgagccag cctggacagg acctgccccg gaccggcctc gaccagggcg cccaccccca
     1321 tgccggccag ggtccgcatc gaggcgtgcc agcggaccgg acggatcacg tggcggccca
     1381 ggagctcgcg gatcgtgttc gggtcctgct cggggccggc ggtcacgttg gccaccaccg
     1441 ggaagaccgg cggtcggata gtcgtacgct ccagcaccgc tcggatcgct tgcagggccg
     1501 gttccatcag cggggagtgg aacgctcccg ccacgttgag ccggatcgcg cgaccgccca
     1561 gtctcctggc ctcgctctcg gcccgagcga cggccggctc cgagccggac aggacgaact
     1621 ggttggcggc gttctcgttc gccaccgcga gcacgtcgtc ccctcgggag gctcggcaga
     1681 cctgatcggc ctgctcctcg gtcaggccga tcagcgccac catggtgccg gggaaccgct
     1741 ccgacgcatc ctgcatggcg gcgccgcgga cccgcacggc ctccagggcc tcgggaaggt
     1801 ccatcgcgcc cgccgccacc agggcggcga actccccgag ggagtgaccg gccgccgcgg
     1861 cgggctcgac gccttcggcc gccaggacgc ggaaggcggc aacgtcgcag gccagcaggg
     1921 cgggttgcac gaacgcggtg gtggccagga gggcctcgtc ccggcaggcg tcgacgatgt
     1981 ctcgaccgag gacctccgag gctacgtcca gcaccgatcg ccccgcctcg tgctccagcc
     2041 acgggtcggc catcgccggg tactgcgacc cttgccccgg gaacaggatc gccagaccca
     2101 ccgcatcatc ctccccaccg gtcgacgccc gaccctatcg tccgggatgc ccgacggcac
     2161 gtacccgggg cggtgggggc gccgcgcgct cgcccgcttg cctcgacgag catcccctgc
     2221 aggttgccag ccctggaccc atcctgtaga acaaggcgcg aacctatggc cgagagggtt
     2281 caacgaggtc ttcccgcatc ccagcgccag cgcaacgcgg gcgccgaggg actgttcggt
     2341 ctcctgggcc aagacatggc cgtcgacctg gggaccgcga acacgctcgt ctacgttcgg
     2401 cgcagcggca tcgtgctgaa cgagccctcg gtcgtggcca tcaacaccct gaccggcgcg
     2461 atcctcgcgg tggggaacga ggccaagcgc atgatcggcc gcaccccctc gcacatccag
//
LOCUS       DASQQQ010000178         5102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_38928, whole genome shotgun sequence.
ACCESSION   DASQQQ010000178 DASQQQ010000000
VERSION     DASQQQ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38928"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VE646_07010"
     CDS             <1..655
                     /locus_tag="VE646_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HZD79774.1"
                     /translation="LAGIVGSINMGNTLGGTNWPGGSLDPETHIVYVQACNACINATS
                     VAPVPPEEGDATYTGGRVDTSTGDDGPRGGLAVQGLPIVKPPYGVLSAIDLDTGELLW
                     QVPHGDTPDDVRDDPALEGIDVPKTGQARSVGLVVTKTLVILGDPEVTSPPGREPGAM
                     LRAYDKETGEEVGAVWLPAPQSGSPMTYMVDGKQYIVVAVSGRRYTGEYIAFALPDA"
     gene            738..1676
                     /locus_tag="VE646_07015"
     CDS             738..1676
                     /locus_tag="VE646_07015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZD79775.1"
                     /translation="MRAVTVREFGPPEVLRVEEVPRPQPGEGELLVRVHAAGVNPVDA
                     GVRSGRARALVGAELPYVPGFDVSGIVAEGGPGVTEFEVGDEVFAMIDLRRGGGYAEY
                     AIVRQEEAASKPARATFEEAAALPLAALTSWQALFDNAGLREGDVVLIHGGSGGVGSV
                     AVQLAKWRGATVIATASERNQDFLRELGADVAIDYQSQRFEDIARDVDVVLDPIGGET
                     QARSLRVLKDGGTLVALIGLTPEARSPGRDIRVRTMLVHPDADQLRRIAGLVDEGRLR
                     PMVSRVFPIEGAPEAHEQIETGHTRGKVVLEIGGSG"
     gene            1741..2568
                     /locus_tag="VE646_07020"
     CDS             1741..2568
                     /locus_tag="VE646_07020"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HZD79776.1"
                     /translation="MSPAVAARLAVVGWGAIGLSRAPAPADGVEGPGVPPPSASSAAY
                     AQEAPYHSYDGSWTFVRVRTHGDYERLGRFRRRFRGYAGWSHDYPDAERNFSRILSEF
                     TSVDVRLVASGGNILTFEDPRIYRFPIAYVSEPDEWRTTAAEAAALRDYLLKGGFVIF
                     DDFFEQEMGNLVRQMRRVFPELDFLPLDGSEAIWGSFFALEPLTIDLEGPRKHGIPRF
                     WGLYMGNDKGRRMLAVAGAGADIGDLWEWSDRGYFPVDPTNEAYRIGINYVIYALTH"
     gene            2750..3700
                     /gene="pstC"
                     /locus_tag="VE646_07025"
     CDS             2750..3700
                     /gene="pstC"
                     /locus_tag="VE646_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HZD79777.1"
                     /translation="MRSPRLSDGIHGRGAGDRVYSVVLTLFALAVPALLLLIFTVVTV
                     GAWPALRRFGFEFVTSTVWDPVAGRFGAGPALYGTLVSSVVALILATPLALGSAIFLT
                     EFAPRRLRRPVAFVVDLLAAIPSVVYGLWGIFVLLPLLRAHVMPFLRDTLHLGATPFF
                     RGPAYGPSMLAAGVILAIMILPYISAVSREVLMAVPRSQREAALALGATRWEMLWGAV
                     IPFAKSGIAGGVILGLGRALGETMAVTMVIGNAARISASLFQPGYTMASLIANEFTEA
                     TGELHLSALLAVGFVLFLVTLVVNAGARWLVWRTSLERGS"
     gene            3697..4554
                     /gene="pstA"
                     /locus_tag="VE646_07030"
     CDS             3697..4554
                     /gene="pstA"
                     /locus_tag="VE646_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZD79778.1"
                     /translation="MSEFRTSPRRRATNALMIGLTWAAAAVATLPLLFILGYLLQKGL
                     SALSWSFFTHLPRPPGVEGGGMANAVVGTLVVVGIASALGVPVGIGAGVYLAEKKGAP
                     LAVVVRFLADVLNGLPSIVIGIFAWELVVVPMGHFSAIAGGFALGAMMIPTVTRTTEE
                     MVRVVPTSLREAALALGYTRWRTALGIVIPTALAGILTGVLVALARIAGETAPLLFTA
                     FGNSFWSLSPTEPIAALPLQIFNYAISPYDEWHAQAWAGALVLIGLLLIIGLISRAVT
                     RSGAVRGVD"
     gene            4598..>5102
                     /locus_tag="VE646_07035"
     CDS             4598..>5102
                     /locus_tag="VE646_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZD79779.1"
                     /translation="MAKAPARMEVRALDAFFGAVHAVRGVSMRIPEHHVTAVIGPSGC
                     GKSTFLRTLNRLHETVPGARVRGRVLLGGEDIYEAEVDPVALRRRVGMVFQRSTAFPT
                     MSIRGNVAAGLRVLRSVGRRVDEEARVKTALRRAALWTEVRDRLDDSALDLSGGQQQR
                     LCIARALA"
BASE COUNT          632 a         1793 c         1862 g          815 t
ORIGIN      
        1 cctcgccggg atcgtcgggt ccatcaacat gggcaacacc ctcggcggca cgaactggcc
       61 cgggggctcg ctggatcccg agacccacat cgtctacgtc caggcctgca acgcctgcat
      121 caacgccacg tccgtggccc cggtccctcc cgaggagggc gacgccacct ataccggcgg
      181 cagggtggac acctccaccg gcgacgacgg cccccggggc ggcctggccg tccagggcct
      241 tcccatcgtc aagcctccct acggcgtgct gtcggccatc gacctggaca ccggtgagct
      301 gctgtggcag gtgccccacg gcgacacgcc cgacgacgtg cgggacgacc ccgccctcga
      361 ggggatcgac gtgccgaaga ccgggcaggc gcggagcgtg gggttggtgg tcacgaagac
      421 cctggtcatc ctcggggatc cggaggtgac gtcgcctccc ggccgggagc ccggcgccat
      481 gctgcgggcc tacgacaagg agaccggcga ggaggtcggc gcggtgtggc tgccggcccc
      541 ccagagcggc tcgcccatga cctacatggt ggacggaaag cagtacatcg tggtggcggt
      601 gagcggccgg cgctacaccg gcgagtacat cgccttcgcc ctgcccgacg cctgaggccg
      661 cgggcggggt gagacgtgcg gggatcgttt tggcggtgct tctcgcgggg ccggggccgc
      721 tccgtgccca gcagacgatg agggccgtca cggtccggga gttcggccca ccggaggtgc
      781 tgcgggtgga ggaggtgccg cgcccgcagc ccggggaggg cgagttgctc gtacgcgtgc
      841 acgcggcggg ggtgaatccg gtggacgccg gggtgcggtc gggccgggcc cgggccctgg
      901 tcggggccga gctgccgtac gtcccgggct tcgacgtcag cgggatcgtc gccgagggcg
      961 ggcccggggt cacggagttc gaggtgggcg acgaggtgtt cgcgatgatc gacctccggc
     1021 ggggcggcgg ctacgccgag tacgccatcg tgcgccagga ggaagcggcc tccaagcccg
     1081 cccgcgcgac gttcgaggag gccgcggcgc tccccctggc ggccctgacc tcctggcagg
     1141 ccctcttcga caacgccggg cttcgggagg gcgacgtggt gctgatccac gggggctccg
     1201 ggggcgtggg ctccgtcgcc gtccagctcg ccaagtggcg gggtgcgacc gtcatcgcca
     1261 ccgcgtcgga gcggaaccag gactttctcc gagagctcgg agccgacgtg gccatcgact
     1321 accagtccca gcgcttcgag gacatcgccc gggacgtgga cgtggtcctg gaccccatcg
     1381 gcggcgagac ccaggcgcga tccctgcggg ttctgaagga cggcggcacc ctggtggccc
     1441 tcatcgggct gaccccggag gcccgttcgc cgggaaggga catccgggtc cgcaccatgc
     1501 tcgtgcatcc cgatgcggat cagttgcgga ggatcgccgg gctggtggac gaaggtcgcc
     1561 tgcgcccgat ggtatcccgg gtcttcccca tcgagggtgc cccggaggcc cacgagcaga
     1621 tcgagaccgg ccacacccga gggaaggtcg tgctcgagat cggggggtcg ggatgagcgt
     1681 gcgcccggcc tgccgcgggg ccccggaggc gggagcgctc cgccttcccg gcgagcgcag
     1741 atgagcccgg ccgtcgccgc ccggctcgcg gtggtgggct ggggtgcgat aggcctctcc
     1801 cgggcaccgg cgcccgcgga cggcgtggag ggacccggcg tgccgccccc ctccgcgtcc
     1861 tccgctgcgt atgcccagga ggctccctac cattcctacg acggctcgtg gaccttcgtc
     1921 cgcgtccgca cccacggcga ctacgagcgc ctcggccgct tccggcgacg tttccgcggc
     1981 tacgccggat ggtcccacga ctatcccgac gcggagcgga acttctcgcg gatcctgagc
     2041 gagttcacca gcgtggacgt ccgcctcgtg gcctcgggtg gcaacatcct caccttcgag
     2101 gacccccgga tctaccgctt tcccatcgcc tacgtctccg agcccgacga gtggaggacc
     2161 acggcggcgg aggccgcggc gctgcgcgac tacctcctca aaggtggctt cgtcatcttc
     2221 gacgacttct tcgaacagga gatggggaac ctcgtgcggc agatgaggcg cgtgttcccc
     2281 gagctcgact tcctgcccct ggacggctcc gaggcgatct ggggctcgtt cttcgccctg
     2341 gagccgctga cgatcgacct ggagggtccg cgcaagcacg ggatcccgcg cttctggggc
     2401 ctctacatgg ggaacgacaa gggccgccgg atgctcgccg tggcgggcgc gggggccgac
     2461 atcggcgatc tctgggagtg gtcggaccgg ggctactttc ccgtggaccc gaccaacgaa
     2521 gcctaccgca tcggcatcaa ctacgtgatc tacgccctga cccactagcc ggggcgacgg
     2581 acggctccgc ggagccgcct gtaccatccc ccggccaggg cccgcgtcgc cgtccgttac
     2641 gtgtccgtta cgtcttcatg acccgttcgc taccggcggc cgctccattt caacccacat
     2701 ggtcgacgga tcccgggccg ggccagccgg cgagccagcg agcgcctccg tgcggtctcc
     2761 acgtctctcc gacggcatcc acggccgtgg ggcgggggac cgggtctatt cggtcgttct
     2821 cacgctgttc gccctggcgg ttcccgcgct cctgctgctg atcttcaccg tggtgacggt
     2881 gggtgcgtgg ccggcgctgc ggaggttcgg cttcgagttc gtcacctcca ccgtgtggga
     2941 cccggtcgcc gggcggttcg gcgccggacc cgccctgtac ggaacgctcg tctcgtcggt
     3001 ggtggcgctg atcctggcca cgcctcttgc gctgggctcg gcgatcttcc tgacggagtt
     3061 cgcaccccgg cggctgcggc ggcccgtggc cttcgtggtg gacctcctgg ccgcgatccc
     3121 cagcgtggtc tacggcctgt gggggatctt cgtgctgctg ccgctgctcc gggcccacgt
     3181 gatgccgttc ctgcgggaca ccctccacct cggagccacg ccgttcttcc gcggcccggc
     3241 ctacggcccc agcatgctgg cggcgggtgt catcctggcc atcatgatcc ttccctacat
     3301 ctcggcggtg tcccgggagg tgctcatggc cgtgccccgg tcgcagcggg aggcggcgct
     3361 ggcgctggga gccacccggt gggagatgct gtggggggcg gtcatcccct tcgcgaagtc
     3421 gggcattgcc gggggcgtga tcctgggcct ggggcgggct ctgggcgaga cgatggccgt
     3481 gaccatggtc atcggcaacg ccgcccggat ctcggcctcg ctcttccagc cgggctacac
     3541 catggcctcc ctcatcgcca acgagttcac cgaagccacc ggagagctcc acctgtcggc
     3601 gctcctggcg gtgggcttcg tcctgttcct ggtgaccctg gtggtgaatg ccggggcccg
     3661 ctggctcgtg tggcgcacgt cgctggagag gggatcgtga gcgagttccg tacctctccc
     3721 cggcggcggg cgaccaacgc cttgatgatc ggcctgacct gggcggcggc ggcggtggcg
     3781 acgctgcccc tgctgttcat cctcggctat ctcctccaga agggcctgag cgccctgagc
     3841 tggagcttct tcacgcacct gccgagacca ccgggcgtgg aggggggcgg catggcgaac
     3901 gccgtcgtgg ggaccctggt ggtggtgggg atcgccagcg ccctcggcgt tcccgtgggc
     3961 atcggcgcag gggtctacct ggcggagaag aagggcgctc ccctcgcggt ggtggtccgc
     4021 ttcctggccg acgtgctgaa cgggctgccg tccatcgtca tcggaatctt cgcctgggag
     4081 ctggtggtgg tgcccatggg ccacttctcc gccatcgccg gcggcttcgc cctcggggcc
     4141 atgatgatcc ccacggtgac ccgcacgacc gaggagatgg tccgggtcgt gccgaccagt
     4201 ctcagggagg cggccctggc gctgggctac acccggtggc gcaccgccct cgggatcgtg
     4261 atccctacgg cgctggccgg catcctcacg ggggtcctgg tggctctcgc ccgcatcgcc
     4321 ggcgagaccg cgcccctgct gttcacggcc ttcggaaaca gcttctggtc gctgtcgccc
     4381 accgagccga tcgcggccct gccgctccag atcttcaact acgcgatcag cccctacgac
     4441 gaatggcatg cccaggcctg ggcgggggcc ctggtcctca tcgggctgct gctgatcatc
     4501 ggcctgatct cccgggccgt gacccggagc ggggccgtcc gtggcgtcga ctgagccggt
     4561 gcggcgcttc tgggcccggg acgtggacgc cgccgccctg gcgaaggcgc cggccaggat
     4621 ggaggtgcgc gcgctggacg ccttcttcgg agccgtccac gccgtgcggg gcgtctcgat
     4681 gcggatcccg gagcaccacg tcaccgccgt catcggtccc tcgggatgcg gcaagtccac
     4741 gttcctgcgc accctgaacc gcctgcacga gacggtgccc ggggcccggg tgcgggggcg
     4801 cgtcctgctg ggaggggagg atatctacga ggcggaggtg gatccggttg ctctccgccg
     4861 cagggtgggg atggtcttcc agcggtccac ggcgtttccc acgatgtcga tccgcggcaa
     4921 cgtggcggcg ggcctccggg tgctccggtc cgtgggtcgg cgcgtggacg aagaggcgcg
     4981 cgtaaagacg gcccttcggc gtgcggcgct ctggacggag gtccgggacc ggctcgacga
     5041 cagcgccctg gacctttcgg ggggccagca gcagcgcctc tgcatcgccc gggccctggc
     5101 ca
//
LOCUS       DASQQQ010000179        10524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_8303, whole genome shotgun sequence.
ACCESSION   DASQQQ010000179 DASQQQ010000000
VERSION     DASQQQ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10524
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8303"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VE646_07040"
     CDS             <1..514
                     /locus_tag="VE646_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD79780.1"
                     /translation="HAFSGGQRQRVGIARALALEPDFIVADEPVSALDVSIRAQVVNL
                     LQDLQDHLGLTYLFIAHDLSVVRHISHRIAVMYAGKIVEVADSDEIYQDPKHPYTRAL
                     LSAVPIPDPALQRDRHRQVLSGEIPNPISPPVGCRFHTRCPMAEERCRTDQPPLEEKS
                     PEHVAACWFT"
     gene            546..2063
                     /locus_tag="VE646_07045"
     CDS             546..2063
                     /locus_tag="VE646_07045"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019608045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZD79781.1"
                     /translation="MSDVQLITHWIDGRPVEGTSDRSGEVFDPAAGDVTKRVGFAGPE
                     DVEAAVRAAQGAFPAWRETPITDRASIMFRFRQLVLDRIEELAALISSEHGKVRVDAM
                     GEIRAGLDVVEFACGIPHLLKGEYSENVSRNINTYSLRQPVGIVAGITPFNFPFMVPM
                     WMTPIALACGNTMILKPSEKDPSASMLVARLLQEAGLPDGVFNVLHGDKVAVEGLLTH
                     PAIGAVSSVGSTPVARYIYETASSRGKRVQALGGAKNHMVVLPDADLDLAADSAVNAG
                     YGSSGQRCMAISAVATVAGAERELLGKIEERIANLKVGPGLDPASQVGPLVTAQSRER
                     VMSLIASGADEGARVAVDGRDVKVEGYENGFFVGPTVIDGVTAEMTVYREEIFGPVLV
                     VAHASRLDEAIRLVNANPYGNGTAIFTNDGGAARKFEHEVEVGMVGINVPIPVPLPFY
                     SFGGWKGSLFGGLHMYGPDGVRFFTRGKVVTSRWPDPSARGVDLGFPGAKRAPTD"
     gene            complement(2067..4370)
                     /locus_tag="VE646_07050"
     CDS             complement(2067..4370)
                     /locus_tag="VE646_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZD79782.1"
                     /translation="MDAWVGRSLRRKEDERFVRGAGRYVADLSFPDQLQVAFVRSPLA
                     HARLGGMDPSAALGTPGVVAVLTHRDVPAGPLPPFLWDAPPEKLVSVLQPMLRPCHPP
                     LLPHDRVRFVGQAVAMVLAESRYAAEDGAERVEVNYDPLPPAATVEDALGPDAPRIHA
                     GWPDNLAVRFTVRKGDPEAAASHAALTVHERYRIQRQAGIPLETRGALALPQDGRLTL
                     WSATQNAHPLRRALSRVTGRPEDTVDVVAPDVGGGFGTKGVLYPEDLLVGLAALRLER
                     PVKWVEDRIEHMQSAIHAREQEHDVRLSVDADGRILALVDHFVVDTGAFNPLGLVIPY
                     NTIAHLMGPYRVPAMEAVGTGVVTTKVPTAPYRGAGRPEAVFAVERSLERAARSLGVD
                     PLEIRLRNLVPPEEMPFDAGILYRDGEPLVLDSGDYQGALRRAASLVRSGAYRSAEEP
                     RDGSLRGVGFACYVEGTGIGPFEGAAVRLDPSGSASVHTGACSQGQSHETVFAQVCAD
                     ALGVDPDDVDVTTGDTRGLERGWGTVASRSAVVAGNAVRAASQRVRSQVLSAASEVLE
                     TAPEDLAIRGARVEVVGAPERSVQLRTLGGRDPNLLAATEYYEPPTVTWANGVHAAEV
                     EVDPSTGELRIVRYVVVHDCGTLINPMVVEGQIHGGVAQGIGGALLEEVVYDRDGQLM
                     NATLADYLVPTAAEVPAIVLDHLESPSPLNPLGIKGVGEGGAVPAAAALANALEDALR
                     STGAVVRRTPLSPSYVRSLLQSSSAGQ"
     gene            4555..5310
                     /locus_tag="VE646_07055"
     CDS             4555..5310
                     /locus_tag="VE646_07055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZD79783.1"
                     /translation="MRELDLGGTRALVSGASRGIGKAIAEGLASAGADVVGLARTAAA
                     LDELGGRIEELGARFLPVAIDVRDPEAVEVAVEDAWRWRGGIGILVNAAGVIVRSEPP
                     AVKPDEFDAVFGTNVRGTFFLTQAVGGRMLEGSGGSIVNVASLAGRVVTGAAVTYQAS
                     KAAIIQLTRALAVRWAPKIRVNAVGPGYIRTDLNTAWLDVEENLRYVHDHTPLGRVGT
                     PADVAGAVIFLASADAAYITGQHLMIDGGWSAQ"
     gene            complement(5318..5866)
                     /locus_tag="VE646_07060"
     CDS             complement(5318..5866)
                     /locus_tag="VE646_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006699719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZD79784.1"
                     /translation="MASQPARDLVDRILEQWERERPDLDPSPMAIIARISRLSRFLER
                     QVESVLAQHGLSEPQFGVLAALRRAGPPYCLSPTALYNSLLISSGAMTNRLERLTAAG
                     FVKRVPDANDRRSILVMLTPKGLRVIEEAVRAHTENEEQLLDPLTGQERQTLARLLRK
                     LVLQFEDGNRLIRQIDSVRESI"
     gene            6091..7098
                     /locus_tag="VE646_07065"
     CDS             6091..7098
                     /locus_tag="VE646_07065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZD79785.1"
                     /translation="MERIGLAAGYDPSMTPRDLAAAFRAAEERGFEVGFFSETIELVR
                     DSVTSLAAIGLGTQSMTLGCTQIVRLRTPLVMAQSLAALDELTGGRMMMAPGACTNTH
                     AKRHGLHEASPPQTLVEWVESIRAILSGETVSYHSEHVHFDEVKLSFSPTRTHVPLFI
                     PATSKTGLQIAGRIGDGVLLNATCSPEYSANAIKIVRQAAEEAGRDWSTFQIWQIINT
                     SVEDDHRKALDAIRWEIASKLDPIQLPFIIKPKKVVGEPYMQEEDIPMFQEAWDRGGK
                     QALIEAVPDSYVEGLTASGTPDEVVKRVQQYRDVGVQVPLLRPAAPHQVDRILELFSP
                     S"
     gene            complement(7170..7832)
                     /locus_tag="VE646_07070"
     CDS             complement(7170..7832)
                     /locus_tag="VE646_07070"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZD79786.1"
                     /translation="MELENTLEIPAPLDRVWGFMLDVEQVAPCMPGAELTEVVDDHTW
                     KGKVNVKLGPVSLSFAGTVVLQDRDEAAHTVVLKADGRETKGKGTASAQVTSKMEAAD
                     GDRTRVSISADLNISGSLAQFGRGMIGDVSQRMANQFAACLGERMQAMEAQAPPAEPV
                     GAAATGEAAPAPPPAGAPLPGAAQPVGGIRLALWALFRALVRGVVRLGKAITGLFKGR
                     SS"
     gene            complement(7941..8249)
                     /locus_tag="VE646_07075"
     CDS             complement(7941..8249)
                     /locus_tag="VE646_07075"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HZD79787.1"
                     /translation="MTVTEVWTHGTWTVRPGSEDDFVDAWRSLAEWTVQEFGTAAAGR
                     LLRDQERANVFISFGPWSDLDTVERWRASEGFRDRVAMITRLLDSFEPRTLEPVTQVP
                     "
     gene            complement(8246..9190)
                     /locus_tag="VE646_07080"
     CDS             complement(8246..9190)
                     /locus_tag="VE646_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79788.1"
                     /translation="MGRRLAERLRTGHRSPPVQVICGHCGARALGAAYLVRSDARWWS
                     IPGRVLRVLLRRRRMWPAPYLYLIAAGLGVVLGAVLAVSVGAVWWWFATLALPTLVWI
                     GFLATAFGDRDRDPTDELLTQIVQEIDHRRGALRYERRRAELFRRAPLPRYGLPPSWP
                     GARSLGGHETSDGRVVALELQHGEPQGPGLRVEVRSPAHPPALAALTEELWHTAHRPP
                     PHLAPERFSAWAQEQERMASSWSPRWEQAPLLLEGRSVVADLVRTGDAFVAVARVDEH
                     ALVIRGIRWDPEGLELGTIVDLDPYVEGSKRALDSWGG"
     gene            complement(9348..>10524)
                     /locus_tag="VE646_07085"
     CDS             complement(9348..>10524)
                     /locus_tag="VE646_07085"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZD79789.1"
                     /translation="LEYLDDFGLPDLEALGEEICNRSERALRQAIRALPDGEYHKVIH
                     TDGIDEPVRIECTVKIHGDEILVDYTGSSPQVSKGMNVVLNYTAAYTSYALKCAISPE
                     VPHNDGSFRPVTTWAPEGSILNPTPPAAVAARHIVGHFLPHAIFGALTPILPHRVLAE
                     GAGNIWLTTVRGTGSDRFITVFFAAGGTGARPTKDGLSTTSFPSGIATAPVEVIETTS
                     PLVFHRKELRTDSGGPGRYRGGLGQSIEVEVRTGEPFVVSSLADRMRFQAEGYLGGKT
                     GGSGGFKTSLGTRPNPKLSQALPPGTRFTLELPGGGGFHDPLTRDPAAVAEDVAQGLV
                     SVASAWRDYGVKVTPAGKITGLDARRRNRAGATGGASAHGKGRGSASRRRTHTGSRH"
BASE COUNT         1581 a         3670 c         3749 g         1524 t
ORIGIN      
        1 gcacgcgttc agcggcggcc agcgccagcg cgtggggatc gcccgcgccc tggccctgga
       61 gccggacttc atcgtggccg acgagcccgt ctccgccctc gacgtctcga tccgggccca
      121 ggtcgtgaac ctgctgcagg acctgcagga tcatctgggg ctcacgtacc tcttcatcgc
      181 ccacgatctg tcggtggtgc gtcacatctc gcaccggatc gcggtgatgt acgcgggcaa
      241 gatcgtcgag gtggccgaca gcgacgagat ctaccaggac cccaagcatc cgtacacgcg
      301 ggcgctgctc tcggcggtac cgatccccga tcccgcgctg cagcgggacc ggcaccggca
      361 ggtgctgtcc ggcgagatcc ccaacccgat ctccccgccg gtaggatgcc ggttccacac
      421 ccggtgcccc atggcggaag agcgatgcag gaccgaccag ccaccgctgg aagagaagtc
      481 ccccgagcac gtggccgcct gctggttcac gtgatcgcgc gatctccagc caccgaggag
      541 gttccatgag cgatgtgcaa ctgatcacgc actggatcga cgggcgaccc gtggagggca
      601 cctccgatcg atccggcgag gtcttcgacc cggcggccgg cgacgtgacc aagcgggtcg
      661 ggttcgcggg gccggaggac gtggaggcgg ccgtccgcgc ggcccagggt gccttccccg
      721 cgtggcgcga aacgccgatc accgaccggg cctccatcat gttccggttc cgccagctgg
      781 tgctggaccg catcgaggag cttgcggccc tgatctcgag cgagcacggg aaggtgcgcg
      841 tcgacgcgat gggcgagatc cgcgccggcc tggacgtggt cgagttcgcc tgcgggatcc
      901 cccacctgct gaagggggag tactcggaga acgtctccag gaacatcaac acgtactccc
      961 tgcgccagcc ggtggggatc gtggctggta tcacgccgtt caacttcccg ttcatggtcc
     1021 cgatgtggat gacgccgatc gcgctggcct gcgggaacac gatgatcctg aagccctcgg
     1081 agaaggaccc ctcggcctcg atgctcgtcg ctcggctgct ccaggaggcc ggactccccg
     1141 acggcgtctt caacgtcctg cacggggaca aggtggcggt cgaggggctg ctgacccatc
     1201 cggcgatcgg cgcggtgagc tccgtcggat ccacgcccgt cgctcggtac atctacgaga
     1261 ccgcctccag ccggggcaag cgcgtgcagg cgctgggggg cgccaagaac cacatggtcg
     1321 tgctccccga cgccgacctg gatctggcgg ccgattccgc ggtcaacgcc ggctacgggt
     1381 cgtccggcca gcggtgcatg gcgatctccg cggtggccac cgtggccggg gcggagcggg
     1441 agctgctcgg gaagatcgag gagcggatcg cgaacctcaa ggtcggtccg ggtctggacc
     1501 ccgcctccca ggtgggccca ctggtgaccg cccagtcccg ggagcgggtg atgtccctga
     1561 tcgcctcggg cgccgacgag ggcgctcggg tggcggtgga cggccgggac gtcaaggtgg
     1621 agggctacga gaacgggttc ttcgtcggcc ccaccgtgat cgatggggtc acggcagaga
     1681 tgaccgtgta tcgggaggag atcttcggtc cggtcctggt ggtggcgcac gcgtcgaggc
     1741 tggatgaggc catccggctg gtgaacgcga acccgtacgg gaacgggacc gcgatcttca
     1801 cgaacgacgg cggtgccgcc cgcaagttcg agcacgaggt cgaggtgggg atggtcggca
     1861 tcaacgtccc gatcccggtg ccgctcccgt tctactcgtt cggcggctgg aagggctcgc
     1921 tgttcggtgg gctgcacatg tacggcccgg acggggtccg gttcttcacc cggggcaagg
     1981 tcgtgaccag tcggtggccc gatccctccg cgcggggggt ggatctgggc ttccccggcg
     2041 ccaaacgagc gcccacggac tgaccgtcac tggcctgccg aggaggactg caggagggat
     2101 cggacgtagc tgggggatag gggcgtccgt cggaccaccg ctccggtgct tcttagggca
     2161 tcctccagcg cgttcgcgag cgccgcggcc gcgggcacgg caccgccctc gcccacgccc
     2221 ttgatgccga gggggttcaa gggcgagggg gactcgaggt gatcgagcac gatagcgggc
     2281 acctcggccg cggtgggcac gaggtagtcg gccaacgtgg cgttcatcag ctgcccgtcc
     2341 cggtcgtaga cgacctcctc caggagcgcc ccgccgatcc cctgagccac cccgccgtgg
     2401 atctgcccct ccaccaccat cgggttgatc agggtgccgc agtcgtggac cacgacgtac
     2461 cggacgatgc gcagttcgcc ggtcgaaggg tcgacctcca cctccgccgc gtggaccccg
     2521 ttcgcccagg tgacggtggg gggttcgtag tactcggtgg cggccagcag gttgggatcg
     2581 cgtccgccga gggtcctcag ctggacggag cgctccgggg cgcctaccac ctcgacccgc
     2641 gccccgcgga tcgcgaggtc ctcgggagcg gtctccagga cctcagaggc ggcgctgagg
     2701 acttgggatc ggaccctctg cgaggcggct cggacggcgt tccccgccac cacggcgctc
     2761 cggctggcca cggtgcccca accacgctcc agccctcggg tgtcgcccgt ggtcacgtcc
     2821 acatcgtccg ggtccacccc cagggcgtct gcgcagacct gagcgaagac cgtctcgtgg
     2881 gactgaccct gggagcaggc gccggtgtgg accgaggccg atcctgaagg atccagccgt
     2941 accgcggcgc cctcgaacgg gccgatgccg gtgccctcca cgtagcacgc gaaccccacg
     3001 ccgcgaaggg acccgtcgcg aggttcctcg gccgatcgat acgcccccga gcggaccagg
     3061 gaggcggccc ggcgcagggc cccctggtag tcgcccgagt ccagcaccag gggctcgccg
     3121 tctcgataca ggatccccgc atcgaacggc atctcctcgg gggggacgag gttgcggagt
     3181 cggatctcca gcgggtccac gcccagcgaa cgggcggcac gctccagcga gcgttccaca
     3241 gcgaagacgg cctcgggccg cccggccccc cggtacggag ccgtgggcac cttggtggtg
     3301 acgacgccgg tgcccacggc ctccatggcc ggaacccggt acgggcccat gaggtgggcg
     3361 atcgtgttgt aggggatcac caggccgagc gggttgaagg cccccgtgtc caccacgaag
     3421 tggtccacga gcgcgaggat ccggccgtcg gcgtccaccg atagccggac gtcgtgctcc
     3481 tgctcacgcg catggatcgc gctctgcatg tgctcgatgc ggtcctcgac ccacttcacc
     3541 ggccgttcca ggcgcagtgc ggccaggccg accaggagat cctcggggta gagcacgccc
     3601 ttcgtcccga agccgccgcc gacgtcgggg gccaccacgt ccacggtgtc ctcgggccgg
     3661 ccggtgacgc ggctgagggc ccggcgcagg gggtgcgcgt tctgcgtggc ggaccagagc
     3721 gtgagccgac cgtcctgcgg gagtgcgagc gctccgcgcg tctccaacgg gatccccgcc
     3781 tggcgttgga tccggtaccg ctcgtggacc gtgagggcgg cgtgcgaggc ggcggcctcc
     3841 gggtccccct tccggaccgt gaaccgcacg gccaggttgt ccggccaccc cgcgtggatc
     3901 ctcggcgcat cgggaccgag ggcatcctcg accgtggcgg cggggggcag aggatcgtag
     3961 ttcacctcga cgcgctcggc gccgtcctcc gccgcgtacc ggctctcggc caggaccatg
     4021 gccaccgcct gtcccacgaa gcgcacgcgg tcgtggggca gcaggggtgg gtggcagggc
     4081 cgcagcatgg gttggaggac gctgaccagc ttctcgggcg gcgcgtccca gaggaaggga
     4141 ggcaaggggc ccgctgggac gtcgcggtgc gtgagcacgg cgaccacgcc cggggtcccc
     4201 agggcggccg agggatccat cccgccgagc cgcgcgtgcg cgagcggact ccggacgaag
     4261 gcaacctgga gctgatcggg gaaggaaagg tccgccacgt agcgcccggc acctcggacg
     4321 aaacgctcat cctccttgcg acgcaaggat ctccccaccc aagcgtccat cacgaccctc
     4381 ggtccttccc ctgccgctcc gcccggccac tggggacgag cgggggacga ccgtctcgac
     4441 gtcgagatac cttacgccgt gcatggggag gggacaaggc ggccgggccg tccttgacag
     4501 ggctcgggag cccaggtatc tagacgtcga gatatcgacc gtcagggggg aaggttgcgg
     4561 gagctggacc tgggggggac cagggcgttg gtgtccggtg ccagccgcgg catcggcaag
     4621 gccatcgccg aagggttggc gagcgccggc gcagacgtcg tagggttggc ccgcacggcg
     4681 gccgccctcg atgagctcgg cggtcggatc gaggagctgg gggcccgctt cttgccggtg
     4741 gcgatcgacg ttcgcgaccc ggaggcggtc gaggtcgccg tagaggacgc gtggcgctgg
     4801 cggggtggca tcggcatcct cgtgaacgct gctggcgtga tcgtgcgcag cgagccaccc
     4861 gcggtgaagc ccgacgagtt cgacgcggtc ttcggcacga acgtgcgggg gaccttcttc
     4921 ctgacccagg cggtgggcgg gcgcatgctc gaaggctcgg ggggttccat cgtgaacgta
     4981 gcatcgctgg cgggccgcgt ggtcaccggc gcggccgtga cctaccaggc cagcaaggca
     5041 gcgatcatcc agctgacacg tgccctcgcc gttcgctggg cgccgaagat ccgggtcaac
     5101 gccgtgggcc ccggctacat ccgcaccgac ctcaacaccg cgtggctgga cgtggaggag
     5161 aacctccggt acgtgcacga ccacacgccg ctgggacggg tggggacccc ggcagacgtg
     5221 gcgggtgcgg tgatcttcct ggcctcggcg gatgccgcct acatcaccgg ccagcacctg
     5281 atgatcgacg gagggtggag cgcccagtga tccgcgatca gatcgactcg cggacggagt
     5341 cgatctgccg gatcagccgg ttgccgtcct cgaactggag cacgagcttg cgcaggagcc
     5401 gagccagcgt ctgccgctcc tgccccgtga gcggatccag tagttgctcc tcgttctccg
     5461 tgtgggcgcg aacggcctcc tcgatgacgc gcagaccctt gggggtgagc atcaccagga
     5521 tgctccggcg gtcgttcgcg tcgggaaccc gtttgacgaa gccggcagcc gtcaggcgct
     5581 ccaggcggtt ggtcatcgcc ccggacgaga tcaggaggga gttgtacagg gcggtgggag
     5641 ataggcagta ggggggaccg gcacgtcgca gggcggccag gaccccgaac tgcggctcgc
     5701 tcagcccgtg ctgggccagc accgactcga cctgacgctc caggaaccgc gagagccgag
     5761 agatccgggc gatgatcgcc atgggagagg ggtccaggtc cggtcgctcc cgctcccact
     5821 gctccaggat ccgatccacc aggtcgcgcg caggctggct ggccatcgcc ccctccctcc
     5881 tcttcaagac gtctcgagat cgagacagac tggaccgccg atccgacggt cgtcaagcat
     5941 ccgccgccgc gaggtcgact tgactggcct cgcgcaccga tactagggta tctgaacgct
     6001 aagatatctc accgagcccg gatcggggag gggacctggg tcgtcgccgg cccgtccggg
     6061 gtcgacggtt cggacgatcc gggggtgggg atggaacgca tcgggctcgc agccggctac
     6121 gaccccagca tgacgccgcg ggatctggcc gcggccttcc gagccgccga ggagcgcggc
     6181 ttcgaggtcg gattcttctc cgagaccatc gagctggttc gcgactcggt cacctcgctc
     6241 gccgcgatcg gtctgggcac ccagagcatg acgctcggat gcacgcagat cgtccggctg
     6301 cggacaccgt tggtgatggc gcagagcctg gccgccctgg acgagctgac cggaggccgg
     6361 atgatgatgg ccccgggggc ctgcacgaac acccacgcga agcggcacgg gctgcacgag
     6421 gccagtcctc cccagaccct ggtggagtgg gtcgagtcga tccgcgcgat cctctccggc
     6481 gagaccgttt cctaccacag cgagcacgtg cacttcgacg aggtgaagct gtcgttcagc
     6541 ccgacccgga cgcacgtccc gctgttcatc cccgccacca gcaagaccgg cctgcagatc
     6601 gccggccgga tcggcgacgg ggtgctgctg aacgccacgt gctctcccga gtacagcgcg
     6661 aatgccatca agatcgtccg tcaggcagcg gaggaggccg gccgggactg gtcgacgttc
     6721 cagatctggc agatcatcaa cacctcggtc gaggacgacc accggaaggc gctggacgcg
     6781 atccggtggg agatcgccag caagttggac ccgatccagc tccccttcat catcaagccc
     6841 aagaaggtgg tgggcgagcc ctacatgcag gaggaggaca tccccatgtt ccaggaggcc
     6901 tgggaccggg gtgggaagca agcgctgatc gaggccgttc ccgactccta cgtggaaggg
     6961 ctcaccgcct cggggacgcc cgacgaggtc gtgaagcggg tccagcagta ccgagacgtc
     7021 ggcgtccaag tcccgctgct gcggccggcg gcaccgcacc aggtcgaccg catcctcgag
     7081 ctgttctccc cgagctgagc ctcgtcaccg cggcggccga tcgctcggcg ccctcgcggg
     7141 catcggcgat ctcaggggag aaggccgatc tagctcgaac gacccttgaa cagcccggtg
     7201 atcgccttgc cgaggcgcac cacgccgcgg accagggccc ggaacagggc ccacagcgcg
     7261 agccggatcc cgcctaccgg ctgggccgcg ccaggaagcg gcgcgcccgc gggcggggga
     7321 gcgggagcgg cctcgccggt cgcggcggcg cccaccggct ctgcgggcgg tgcctgcgcc
     7381 tccatggcct gcatccgctc gcccaggcag gcggcgaact ggttcgccat ccgctgggag
     7441 acgtcgccga tcatcccgcg gccgaactgg gccagcgaac ccgagatgtt cagatcggcc
     7501 gagatggaca ccctggtgcg gtcgccatcg gccgcctcca tcttcgaggt cacctgggcc
     7561 gaggcggtgc ccttgccctt ggtctcccgc ccgtcggcct tcagcaccac ggtgtgggcg
     7621 gcttcgtcgc ggtcctgcag cacgacggtg ccggcgaacg agaggctgac cggacccagc
     7681 ttcacgttca ccttcccctt ccaggtgtgg tcgtcgacga cctcggtgag ctccgccccc
     7741 ggcatgcacg gagccacctg ttccacgtcc agcatgaatc cccacacccg gtccagcggg
     7801 gctgggatct ccagcgtgtt ctcgagctcc acggttcccc ctccgtcgca ccgcagcgcg
     7861 ctgccgcgct gcctccagat atctctatgt taagttagtt ggtcctctcg gcgccagccg
     7921 gtgtcggggc gggagcccgg tcagggcacc tgggtgaccg gctccagggt cctcggttcg
     7981 aacgagtcca ggagccgcgt gatcatcgcc acgcggtcac ggaacccctc cgaggcgcgc
     8041 caccgctcga ccgtgtcgag gtccgaccag gggccgaagc tgatgaagac gttcgcgcgc
     8101 tcctggtccc gcaacagtct gcccgccgcc gccgtgccga actcctggac cgtccactcc
     8161 gccaacgacc tccacgcatc cacgaagtcg tcctccgacc ccggcctcac cgtccacgtc
     8221 ccgtgcgtcc agacctcggt caccgtcatc ccccccagct gtcgagggcc cgcttcgatc
     8281 cctcgacgta gggatcgaga tccacgatcg taccgagctc gaggccctcc gggtcccatc
     8341 ggatgccccg gatcacgagc gcgtgctcat ccacgcgcgc caccgccacg aacgcgtccc
     8401 cggtgcggac gaggtccgcg acgaccgatc gtccctccag gaggagcggg gcctgctccc
     8461 agcgaggcga ccagctcgat gccatccgtt cctgctcctg ggcccaggcg ctgaagcgct
     8521 cgggcgccag gtggggcggt gggcgatggg ccgtgtgcca gagctcttcg gtgagcgcag
     8581 cgagagcggg aggatgggcg ggggatcgga cctcgacccg gagccccggg ccctggggct
     8641 cgccgtgctg cagctccaga gccacgaccc ggccgtccga cgtctcgtgg ccgcccaggg
     8701 agcgagctcc cggccaggac ggaggcagtc cgtaccgggg aagcggtgcg cggcggaaga
     8761 gctcggcgcg acgacgctcg tagcggagcg cgccacgtcg atgatcgatc tcctgcacga
     8821 tctgcgtgag gagttcgtcg gtcggatcac ggtcccgatc tccgaaggcg gtcgccagga
     8881 acccgatcca gaccagggtc ggcagcgcaa gtgtggcgaa ccaccaccac acggcgccga
     8941 cggatacggc cagcacggcg ccgaggacca caccgaggcc ggctgcgatc aggtacaggt
     9001 agggggccgg ccacatccgc cgacggcgca ggagcacgcg gagcaccctg cccgggatcg
     9061 accaccaccg ggcgtccgag cgaaccaggt aagcggcccc gagggcgcga gcaccgcagt
     9121 gcccacagat cacctggacg ggcggcgagc gatgccccgt ccggagcctc tcggccaggc
     9181 gcctacccat ggatccgtgc tccgggcgcc agtcctcggc gccgcagttc gggcaggggc
     9241 cccggttacc taccgtgacc gcccaccacc gcccaccact cgcccttccc gtgagccata
     9301 tccgaaggat ggcacgggag accgagccgg cgggaggcgt ccgctggcta gtgacgggag
     9361 cctgtgtggg tccgccggcg ggatgccgag ccccggccct tgccgtgcgc ggatgctcca
     9421 ccggttgccc cggcgcggtt gcggcgtctg gcgtcgagcc ccgtgatctt cccggccggc
     9481 gtgaccttga cgccgtagtc cctccaggcc gaggccacgc tcaccaggcc ctgggcgacg
     9541 tcctcggcca ccgcggcggg atctcgcgtg agggggtcgt ggaagccgcc gccccccggc
     9601 agctcgagcg tgaaccgcgt tccgggcggc agcgcctggg agagcttggg gttcgggcgg
     9661 gtgccgagcg aggtcttgaa gccgccgctg ccgcccgtct tgccgcccag gtagccctcg
     9721 gcttggaagc gcatccggtc ggccagcgag gagacgacga acggctcgcc ggtgcggacc
     9781 tcgacctcga tgctctgtcc gaggccaccg cggtagcgtc cgggtccgcc ggaatcggtc
     9841 cggagctcct tgcggtggaa caccaggggc gaggtggtct cgatcacctc gaccggcgcg
     9901 gtggcgatgc cgctggggaa gctggtggtg gacagcccgt ccttggtggg acgggccccg
     9961 gtgcccccgg ccgcgaagaa caccgtgatg aaccggtccg agccggtccc gcggacggtg
    10021 gtgagccaga tgttgccggc cccctcggcc aggacccgat gcggcaggat cggcgtgagc
    10081 gcgccgaaga tggcgtgcgg caggaagtgg cccacgatgt gccgggccgc cacggccgcc
    10141 ggcggcgtgg ggttcaagat cgagccctcc ggcgcccagg tggtcaccgg gcggaagctc
    10201 ccgtcgttgt ggggcacctc gggcgagatg gcgcacttca aggcgtagct ggtgtaggcg
    10261 gccgtgtagt tcagcaccac gttcatgccc ttgctcacct gcggtgagct ccccgtgtag
    10321 tccacgagga tctcgtcgcc gtggatcttc acggtgcact cgatccgcac cggctcatcg
    10381 atcccgtcgg tgtggatcac cttgtggtac tcgccatcgg ggagcgcccg gatcgcctgg
    10441 cgtagcgcgc gctcggagcg gttgcagatc tcctcgccca gcgcctccag gtcgggcaga
    10501 ccgaaatcgt ccaggtactc cagc
//
LOCUS       DASQQQ010000180         2343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_8329, whole genome shotgun sequence.
ACCESSION   DASQQQ010000180 DASQQQ010000000
VERSION     DASQQQ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8329"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..732
                     /locus_tag="VE646_07090"
     CDS             166..732
                     /locus_tag="VE646_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79790.1"
                     /translation="MLAAAGVVLPAACADPGTGPGDGVAYFPLETGHVWTYAPADPLY
                     GQPFQWQVVERRGDTVTLARPPAGSHPGPVTLVDGVDGVDLLLESGTIVPFYRFTAGT
                     SWVHHDPWECDDGSEWAAVDEGDAIATPAGTFLHTLRLERRSAASCTDAGTTAEWWAP
                     DVGLVRWEELNFYAGGPLTFDLVGHSRR"
     gene            887..1858
                     /locus_tag="VE646_07095"
     CDS             887..1858
                     /locus_tag="VE646_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZD79791.1"
                     /translation="MRRVPLGTTGLEVSELCFGTMTFGRASDEKTSADVYARCREAGI
                     DFFDCANVYAEGESERILGRLVAAERDRVILTSKVYFPAGEGPRARGYSRAAILHQLE
                     GSLERLGTDYLDLYFLHHFDEDTPIEDALRTLDDLVRDGKVRYPAVSNFAAWRIARAL
                     GTSERLALAPFRCVQPMYNLLKRQAEVEILPMAEAEGLAVTPYSPMAGGVLTGKYGDK
                     DLSVEGRLKTSDMYQKRYGDPRNFEVARRFAGLAGDHGWHPAALAVAWVARHPAVTAP
                     IIGARNLEQLESTLGAVDIELDDDTYAEISALTPEPPLATDRAEERM"
     gene            complement(1865..>2343)
                     /locus_tag="VE646_07100"
     CDS             complement(1865..>2343)
                     /locus_tag="VE646_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZD79792.1"
                     /translation="SAIAAVDASIPTGSLEPLRQRALRSVRREHVLAWLMGAFSGVAL
                     VLSVVGMFGALSNLVLRRRPELGVRAALGASPLDLRRLVLARAGLMLGAGTVGGLLIA
                     SLLGRLLAGLLFGVAPGDPVTLLAAAGTLALAGALASLVPSSKAGHTDPARLLNAE"
BASE COUNT          372 a          829 c          837 g          305 t
ORIGIN      
        1 ccccggccga gcctcgcggg ggtcgccctc agggacggcc tccccgtgcg ccatccgtgg
       61 agaggagccg gatccgggcg gcccgggacg aggtgaccca tgacgggaac gggagaggtg
      121 ctcaggagaa gacggcgtcg gcgaagacgg accgtcccac ggtgggtgct cgccgccgca
      181 ggcgtggtgc tcccggccgc ctgcgccgac ccggggaccg gccccgggga cggcgtcgcg
      241 tacttccccc tggaaacggg ccacgtctgg acctatgcgc ctgcggaccc cctgtacggg
      301 cagccgttcc aatggcaggt cgtcgagagg cgcggcgaca ccgtcacgtt ggcccgcccc
      361 ccggccggat cccatccggg ccccgtgacg ctggtggacg gagtcgacgg cgtcgatctg
      421 ctcctcgaaa gcgggaccat cgtgccgttc taccgcttca ccgccggaac gtcctgggtg
      481 catcacgacc cgtgggaatg tgacgacggt tcggagtggg ccgcggtcga cgaaggggac
      541 gccatcgcca cccctgccgg gacctttctc cacacgttgc gcctcgagcg gcgcagcgcc
      601 gcctcctgca cggacgccgg aaccaccgcc gaatggtggg cgcccgacgt ggggctggtt
      661 cggtgggaag agctgaactt ctacgcgggg gggcccctca cgttcgacct cgtcggccat
      721 tcccggcgct gagcgccgtc ccccggacgc gccgtcggcc cggcgctgcg cgggcatggt
      781 cccggagcgg gcacggccgg gatcgacgtc cgcgatcgag gtttcccgaa acgggaccgt
      841 cccgcatgtt gcaggcttcc acgacgatga cggcaggagg tcgacgatgc ggcgggtccc
      901 cctcggcacg acgggtctgg aggtctcgga gctctgcttc ggcacgatga cgttcgggcg
      961 ggcgtccgac gagaagacga gcgcggacgt ctatgcccgg tgccgggagg cgggcatcga
     1021 cttcttcgac tgcgccaacg tctacgcgga gggggagtcg gagcgcatcc tgggccggct
     1081 ggtggcggcc gagcgcgacc gggtgatcct gacctccaag gtctacttcc ccgccggaga
     1141 aggacccagg gcccggggct actcgcgggc agccatcctg caccagctcg aggggagtct
     1201 ggagcggctc gggaccgact atctggacct gtacttcctg caccatttcg atgaggacac
     1261 ccccatcgag gacgccttgc gcacgttgga cgacctggtg cgggacggca aggtccgcta
     1321 cccggcggtg agcaacttcg ccgcgtggcg gatcgcccgg gctctcggca cctccgaacg
     1381 gctggcactg gcccccttcc ggtgcgttca gcccatgtac aacctcctca agcgccaggc
     1441 ggaggtggag atcctgccca tggccgaggc ggagggcctc gccgtcaccc cctacagtcc
     1501 catggccggc ggcgtgctca ccggcaagta cggtgacaag gacctctccg tggagggtcg
     1561 gctgaagacc agcgacatgt accagaagcg ctacggcgat ccgcgcaact tcgaggtcgc
     1621 ccggaggttc gccggcctcg ccggggacca tggctggcat ccggcggccc tcgcggtggc
     1681 gtgggtcgcc aggcatccgg ccgtaaccgc cccgatcata ggtgcccgca acctggagca
     1741 gctcgagtcc accctcggcg ccgtggacat cgagctggac gacgacacgt acgccgagat
     1801 ctcggccctc acgccggaac cacctctggc cacggaccgc gcagaggagc ggatgtagag
     1861 ccggctactc ggcgttcagc agcctcgccg gatcggtatg ccccgccttc gaggaaggaa
     1921 ccaggctcgc cagggcaccg gcgagtgcga gcgtacccgc cgcggcgagc agcgtcacgg
     1981 gatcgcctgg agccacgccg aagagcaggc cggccaggag acgtcccagc agcgaggcga
     2041 tcaggaggcc gcccacggtg ccggctccga gcatgaggcc cgcacgggcc aggaccagcc
     2101 gacgaaggtc gagaggactg gccccgagcg ccgcccgaac cccgagctcc ggcctccggc
     2161 gtagcacgag gttggacagg gcgccgaaca tgcccaccac cgaaagaacc agtgcaactc
     2221 cgctgaaggc ccccatcagc caggcgagaa cgtgctcgcg ccggaccgac cggagagccc
     2281 gctgccgcag gggctcgagg gatcccgtgg ggatcgaggc atccaccgcg gcgatggccg
     2341 atc
//
LOCUS       DASQQQ010000181         3850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_39081, whole genome shotgun sequence.
ACCESSION   DASQQQ010000181 DASQQQ010000000
VERSION     DASQQQ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39081"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="VE646_07105"
     CDS             complement(<1..1315)
                     /locus_tag="VE646_07105"
                     /inference="COORDINATES: protein motif:HMM:NF017400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HZD79793.1"
                     /translation="MVPRTDAGHTVSAIEGVVLMRNPRCRDGHVRWLLTMGSVALSLL
                     LPFIVAPPASAEAHCSSPPEVFPIDDLVPGMTATGLTTISGSTPTSFQVEILGVLPDY
                     IWLDVDTIVVHLTGPDSFLDQVGGVFYGMSGSPVSKDGKLIGAVSYAVSEDPTIFGLT
                     PAQAMVDVLTRAPDAAEALPSKIPFDAATRRAVASAIATPVTEAPSGLQRLPTYLGVS
                     GLSGTMLTRLQRSLDRHGSGMTATSAGGMRSGLPVRDTPFAPGEPVGSVLSWGDMTIW
                     AAGTVTLTCDEGLMAYGHSLFYDPPGELRIGMTGVDVVAVGNGQGLWADSMIPVLTEP
                     RGTFEQDRFTGQLGVVGEQSPSMPITSEFSSLDTGRSRSGETDAIFQDLWYGDYEVWG
                     HMVLNLGAVQQEVGPGTVRYGYTIDGTRADGSAFTVENRTMAYSDS"
     gene            complement(1550..2331)
                     /gene="xth"
                     /locus_tag="VE646_07110"
                     /pseudo
     CDS             complement(1550..2331)
                     /gene="xth"
                     /locus_tag="VE646_07110"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
     gene            complement(2371..3381)
                     /locus_tag="VE646_07115"
     CDS             complement(2371..3381)
                     /locus_tag="VE646_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZD79794.1"
                     /translation="MVHNPGGPDRADGGPTEPKVALAFGPIPSRRLGRSLGINNIPPK
                     VCSYGCLYCQVGATPNPQLEPRSIYPPEVLAAAVARQVDLVRARGEPIDHLTFVPDGE
                     PTLDANLGRAIELLRPLRIPIAVISNGSLAWRPDVRNALRAADWVWVKVDAVEEAVWR
                     RMNRPPGELSLRTVLEGIRALAEGLQGRLVSETMLLEGVNDTEPSLRGVAAFLEAAGI
                     GTGYVAIPTRPPSERGVGGPSEAVVCRAHAILAERVPRVEYLIGYEGDAFAASGDARA
                     DLLAITAVHPMRDSAVRAMLARSGSGWDVVDELVREGLLVPLEHGGERFYVRRFHERP
                     RS"
BASE COUNT          596 a         1351 c         1331 g          572 t
ORIGIN      
        1 cagagtccga gtacgccatg gtgcggttct ccacggtgaa cgccgaccca tccgctcggg
       61 tcccatcgat cgtgtagccg tagcggaccg ttcccggccc gacctcctgc tggaccgcgc
      121 cgaggttcag caccatgtgc ccccacacct cgtagtcccc gtaccagaga tcctggaaga
      181 tcgcgtcggt ctctccactc cgggagcgac ccgtgtcgag gctcgagaac tccgaggtga
      241 tcggcatcga gggcgactgc tcgccgacca cacccagctg tccggtgaac cggtcctgct
      301 caaaggtgcc tcgtggctcg gtcagcacgg ggatcatgct gtccgcccac agcccctgcc
      361 cgttgccgac ggccaccacg tccaccccgg tcatcccgat gcggagctct ccgggcgggt
      421 cgtagaacag cgagtgtccg taggccatca gcccctcgtc acaggtcagc gtcacggtcc
      481 ccgcggccca gatcgtcatg tcgccccagg acagcaccga gcccaccggc tcacctggag
      541 cgaacggcgt gtccctcacg ggcaggccgg atcgcatgcc gccggcggac gtggcggtca
      601 tccccgagcc gtgccggtcc agactccgct gcagccgggt cagcatcgtc cccgatagcc
      661 ccgagacgcc caggtaggtg gggagccgct ggaggccgga gggagcctcg gtcacgggcg
      721 tggcgatcgc ggaggccacc gcgcggcgcg ttgcggcatc gaatgggatc ttcgagggga
      781 gtgcctcggc agcatcaggt gccctggtca gcacgtcgac catcgcctgc gcaggggtca
      841 gaccgaagat cgtggggtcc tcggacaccg cgtaggagac cgcgccgatc agcttgccgt
      901 ccttggagac cggggagccc gacatcccgt agaagacccc acccacctgg tcgaggaagg
      961 agtcgggacc ggtcaggtgc accacgatcg tgtcgacatc gagccagatg tagtcgggga
     1021 ggacgccgag gatctcgacc tggaaggagg tcggagtgga gcccgagatc gtggtgagac
     1081 cggtcgccgt catccccggc acgaggtcgt cgatcgggaa cacctcgggt gggctcgagc
     1141 agtgggcctc ggcgctcgcc gggggcgcga cgatgaaggg cagcagcagc gacagggcca
     1201 ccgatcccat cgtcaggagc cagcggacat gtccatcacg gcagcgcggg ttgcgcatga
     1261 ggaccacccc ctcgatcgcg ctcaccgtat gcccggcatc ggtgcggggc accatcgctt
     1321 gtgccccctc cgggggggac catggacccg ctcgttgcga ggacgttgga gtcctacggc
     1381 gcgcggttcg aatctcgggc caggatgggc ggtccgtctc cccaccgctg gatggatctg
     1441 gtcgatggcc tcagcaacgg ccgccggggc acacgcctca cagccgaggg ggcgagccgc
     1501 tccaggatcg ccgcgttacg tcgtccgcca cggagacggt gaggtcttct catcccagcg
     1561 tcacgatgac gggggcgtga tcgctcggct tgccttcccc gaaggtgggc ttgcgctcgt
     1621 cgcggtccac gcacacctcg gtgcagcgct cggcgaccgg gcgcgtcccg agcgcaaggt
     1681 cgaggcgcag cccccatccc cggtggaaga cgccctcccg gtagtcccac cagctgaacg
     1741 ggccgggacc ggtctcgtgc aggcgcatca ggtccacgaa cccccagtcc aagagggctc
     1801 cgatccgctc gcgctcgggc tcgctgcaca ggttctgccc gcgccaccgc tcgggatcgt
     1861 ggacgtcgcg atcctcggga acgacgttga agtcgcccac cacgagcagg ggctcgcccg
     1921 gatcgtgcgc cgtgcggagc cacgcggtga gggtgtcgag ccaccgcagc ttctgctcgt
     1981 actcccgcgt gccgacggcg cggccgttca ccacgtggac gtcgacgatg cggaccccgt
     2041 ccacggtgcc cgacaccacg cgcgcctgct cgggaagcgg atcccctgcg aagccgtggg
     2101 tgacatcggc caggggcgat cgcgagagca ccgcgacgcc gttgcggccg gcttgacccg
     2161 tgaacggcag ctcggtaccc gagctcctcg aacggggcgg tggggaacgc cctgatcggt
     2221 caccttgagc tcctgcaagc agagcaggtc cggtgcgtgg cgcaggagca gttgcctcgc
     2281 gggggcaagg cccatcttga tcgagttcac gttccaggtc acgaccttca tccgctgctc
     2341 cccttcccgc ccccggtgtg gaccaggggt tcatgaccgt gggcgctcat ggaagcgccg
     2401 gacgtagaat cgctcgcccc cgtgctccag ggggacgagg agcccttcac gcaccagctc
     2461 gtccacgacg tcccaccccg acccgctccg cgcgagcatc gcccgaacgg ccgaatcgcg
     2521 catcgggtgg acagccgtga tcgccagcag atccgccctt gcatcgccgg aggccgcgaa
     2581 ggcgtccccc tcgtagccga tcaggtactc gacgcgtggg acacgctcgg ccaggatcgc
     2641 gtgcgctcgg caaacgaccg cctcactcgg accgcccacc ccccgctctg agggtggtcg
     2701 ggtggggatg gccacgtacc cggtcccgat cccggccgcc tccaggaacg cggcgacgcc
     2761 tcgaagggag ggttccgtgt cgttcacgcc ctcgaggagc atcgtctcgg agaccagccg
     2821 tccctgtaac ccctccgcga gggcccggat cccctcgagc acggtccgca gtgagagctc
     2881 gcccggcgga cggttcatcc gacgccacac ggcctcctcg accgcatcga ccttcaccca
     2941 gacccagtcg gcggcccgca gggcgttgcg gacatccggg cgccaggcca gggagccatt
     3001 ggagatgacg gcgatcggga tccggagcgg gcgaagcagc tcgatcgcac ggccgaggtt
     3061 cgcgtccagg gtgggttccc catcgggaac gaacgtcagg tggtcgatcg gctctccgcg
     3121 ggcgcggacc aggtcgacct gacgagcgac cgcggcggcg aggacctcgg gcgggtagat
     3181 cgatcggggc tcgagctggg ggttgggcgt ggcgcccacc tggcagtaca ggcagccgta
     3241 gctgcagacc ttcgggggga tgttgttgat cccgaggctg cgaccgagcc gccgtgacgg
     3301 tatcggaccg aaggccaggg cgaccttcgg ctcggtgggg cctccatccg ccctatctgg
     3361 accacccggg ttgtgcacca tccacccctc cgcacccacg ctagcggggt cggcgacggt
     3421 cgcgtccggt ggaaggtcac ggctcatgtg ggacgaccgg cctggtgcgg ggagatgcag
     3481 atgcgatgtt atgcggtggc ttcgccgggc ctccttctgc tcgatccctg cccctcggtc
     3541 gaggacttac gacgcctgct cgcggaagcg ctcacggggc tgggcgacct ccgctgctag
     3601 atgttcgtgt cggagggggg acttgaaccc ccacgggcta ttcgcccact aggccctcaa
     3661 cccggaacgg tacgtgtggc ctgacgtcat ctgatgtcgt ccactgcgcc tgagcagggg
     3721 aaacgcatgg acctagtggc gctcgtggtg gcccgggatc ccggctacgc ggcggggcac
     3781 gggaatctca cgggaatctg ctgccccgcg ggaaccatgt ggctccgcga cgccggcgag
     3841 ggccaggtgc
//
LOCUS       DASQQQ010000182         5290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_208828, whole genome shotgun sequence.
ACCESSION   DASQQQ010000182 DASQQQ010000000
VERSION     DASQQQ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208828"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VE646_07120"
     CDS             complement(<1..716)
                     /locus_tag="VE646_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZD79795.1"
                     /translation="MADVQQNISALLSEDRVFEPPADFTSHALVKDGSIYERAEADYE
                     GFWAGQAERLAWSKKWDTVMDWTPPTVKWFVGGKINASYNCLDRHVEAGGGDKVAYYW
                     EGEPGEQRTLTYAEVLDEVSRFANALRSLGVQKGDRVAIYLGMIPELPIAMLACARIG
                     APHSVVFGGFSSDALRDRVNDGEAKVLITQDGGYRRGSVVPLKANADEALKGCPSIEH
                     VITVRRTGGDHPFTPGRDLW"
     gene            939..1091
                     /locus_tag="VE646_07125"
     CDS             939..1091
                     /locus_tag="VE646_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HZD79796.1"
                     /translation="MATYEAYCVKCRAKREFEGQEVTLKNGRKAAQGTCPVCGTKVMR
                     ILGKAS"
     gene            complement(1141..1614)
                     /locus_tag="VE646_07130"
     CDS             complement(1141..1614)
                     /locus_tag="VE646_07130"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZD79797.1"
                     /translation="MLKISQKLEYAMRAMIELAVRQEERSLVPAREIAEAQQIPVRFL
                     EQQLGALNKAGLVQSFRGAGGGCRIAKDPSEIRVSDIADAIEGQLFPMFCLEPTDHTC
                     FQDSRCGLQGFWGDVAMAIKRVFEETTVQDLVERQQRAHRHRPTFSAEELLTRLN"
     gene            1874..3364
                     /locus_tag="VE646_07135"
     CDS             1874..3364
                     /locus_tag="VE646_07135"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HZD79798.1"
                     /translation="MSRRPSVFDAPLIVASNRGPVTFERDEEGELVPRRGSGGLVTAL
                     LGALQEAKGLWVATAMSDGDREMTARSNGHVDMGEFGNSYRLRYLEIDPDDYDAYYNQ
                     ISNGLLWFVHHYLWDTVRSPTFDERTEQAWSAYVRVNRMFAQALAEEADALGEDPVYL
                     IQDYHLALVPRFLRELRPNALIAHFSHTAIAGASYLRVLPGKIREGLLRGMLGADVLG
                     FHSQAWAENFLMSIRLLSGARADLARSRVTIEGREVAIRTHPISVDAAPMRELATTRE
                     IRARRRELNRWRGNARLILRVDRLELTKNIVRGFQAHEVFLRRNPHWRGRVRFLALLS
                     PSRIELEEYRAYGEECVAEAERINRELGDLEWRPIEVQVREDFPGAVAAYGLYDVLMV
                     NPIYDGMNLVSMEGPLLNRRHGVLILSRNAGAYWRLGRYAVAVNPFDLHEQAEAIEEA
                     LEMPADERARRARGLSRLVLSNPPARWVRSQLEDLERARVRRARTA"
     gene            complement(3374..3925)
                     /locus_tag="VE646_07140"
     CDS             complement(3374..3925)
                     /locus_tag="VE646_07140"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HZD79799.1"
                     /translation="MFGRHEPPTNRSFYVSVGTSILRFVIVLALVVGGVVLLNQAFPS
                     GGTIAAPGGGGSTVPTSPPASPGDGKSSSKPPPTPKVVGVKIAVFNAAGVNGLAGDTA
                     LLLQRKFGYKAVQVADAPSTASETEVFYRSPPDKIEAEEMVRLYFKSVDPQISRLDPS
                     ATGVKKNVQVAVYLGTDFADLQK"
     gene            complement(4004..>5290)
                     /locus_tag="VE646_07145"
     CDS             complement(4004..>5290)
                     /locus_tag="VE646_07145"
                     /inference="COORDINATES: protein motif:HMM:NF044969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79800.1"
                     /translation="ILLGIAVQRGALTLEERDHLLHQVEQDVVRHVLYDNFLQAQILS
                     QEADPSSSRMEAYEDLMRSLEDGGHLDRALEALPRTDEMAERRRAGIGMARPELAVLL
                     AYAKIALTNALLDSPLPDSEYLAADLRGYFPPAVVERFGDLLSEHPLRRELVSTIVAN
                     DVVNSQGITFVSRLVTETGAEPSDVVRAYRIARDVTDAVERWEAIEALVGSVEPAILG
                     ELMPGVDRMVETTARWYLAHLPGKLGSAIEAHREPFRRFIDVVERVVPGGWSEDRERI
                     AHRLTDQGVPQTLARRHVLQPLLVHGPNVIVVASATGRSVEDTARAFFAVGEALYIDW
                     LEGRLSDLRATSRWQRWAVQAMEDDLLLVRRQVAERVLSEADDGPIEQAIARFADART
                     EVVARLARFMRALALEDVGDLAAMTVAVRQIRSLTA"
BASE COUNT          827 a         1833 c         1821 g          809 t
ORIGIN      
        1 taccagaggt cccggccggg cgtgaaggga tggtcgccgc cggtccgccg cacggtgatg
       61 acgtgctcga tcgacgggca tcccttcagc gcctcgtcgg cgttcgcctt cagcggcacc
      121 accgatccac ggcggtagcc accgtcctgg gtgatcagga ccttcgcctc gccatcgttc
      181 acccggtcgc gcagcgcatc ggaggagaac ccgccgaaca ccaccgagtg gggggcaccg
      241 atccgggcac aggccagcat cgcgatgggg agctccggga tcatgccgag atagatcgcc
      301 acccggtcac ccttctgcac acccagcgac ctcagagcgt tcgcgaaccg agatacctcg
      361 tccagcactt cggcgtaggt gagggtgcgc tgctcgccgg gctctccctc ccagtagtag
      421 gcgaccttgt ccccgccacc ggcctcgaca tgccgatcga ggcagttgta cgaggcgttg
      481 atcttgcctc ccacgaacca cttcacggtg ggcggcgtcc agtccatcac cgtgtcccac
      541 ttcttggacc aggccaagcg ctcggcttgc ccggcccaga agccctcgta gtcggcttcg
      601 gcccgctcgt agatcgaccc gtccttcacc agggcgtgcg aggtgaagtc cgcgggcggc
      661 tcgaagacgc ggtcctcgga caggagcgcg gagatgttct gctggacgtc tgccatgttg
      721 gttcgccccc tcgatgtggg ttctcctccg catcgtagcc tcgcctcacc cggaaccgca
      781 ccccgccacc cggcccccga cggccccgcc gaccggcccc ccacgcaagc tggggtccgg
      841 gcgcaggtgc ttgcatccgc gcccgggacg gaccagcatc gagggcgagg ccgccgatgg
      901 ggcggcctcg aaggctatcc ggggaaggag gaggcggggt ggccacgtac gaggcgtact
      961 gcgtgaagtg tcgggccaaa cgggagttcg aggggcagga agtgacgctc aagaacggcc
     1021 gcaaggccgc tcagggcacc tgcccggtct gtggcaccaa ggtcatgcgg atcctgggga
     1081 aggcctccta gcgctccggt tcgcgggcgc tcccagcgcc gatccgcccg cccaggacgc
     1141 tcagttcagg cgggtgagca actcttcggc ggagaacgtg gggcggtgac ggtgagcccg
     1201 ctgttgccgc tccacgaggt cctggacggt cgtctcctcg aagacgcgct tgatcgccat
     1261 ggccacgtcc ccccagaacc cctgcagacc acaccgggaa tcctggaagc aggtgtgatc
     1321 ggtcggctcc aggcagaaca tcgggaacag ctgcccctcg atggcgtcgg cgatgtcgga
     1381 cacgcggatc tctgaggggt ccttggcgat ccggcaacca ccgcccgccc ctcggaagct
     1441 ctggaccagc ccggccttgt tgagggcccc gagctgctgc tccaggaaac ggaccgggat
     1501 ctgctgggcc tccgcgatct cacgcgccgg gaccagcgag cgctcctcct gccggacggc
     1561 gagctcgatc atggcccgca tcgcgtattc gagcttctga gagatcttga gcaccgtacg
     1621 gcctccacgt tcaatctatg gacgacggag acctgtgtca acgtggtgca accccccagc
     1681 gcccgacgtt accgcaggtc agacaccatg agcgcaaccc gcacgggcac cgagcgcccg
     1741 gtggcggagc agggcgcggg aggccgcgcg cacccgaacc gatagactcg gaagcggtga
     1801 ctgtatcggt ccgtcgtcca tctcggtctg ccggcgtgcg ccgtgggctg ggttccccga
     1861 gggtgatggg cgcgtgagcc gccgcccctc cgtcttcgat gcccccctga tcgtggcctc
     1921 gaaccgcgga ccggtcacgt tcgagcggga cgaggagggc gagctggtac ctcggcgcgg
     1981 gtcgggcggc ttggtcactg cgctgctcgg cgccctgcag gaggcgaagg gcctctgggt
     2041 ggcgaccgcg atgtcggacg gggatcggga gatgaccgct cggtccaacg gtcacgtgga
     2101 catgggggag ttcggcaaca gctaccggct gcgatacctg gagatcgatc ccgacgacta
     2161 cgacgcctac tacaaccaga tctccaacgg gctgttgtgg ttcgtgcacc attacctgtg
     2221 ggacacggtg cgctccccca ccttcgacga gcgcaccgag caggcatggt ccgcgtacgt
     2281 tcgggtgaac cggatgttcg cccaggccct ggccgaggag gcggatgccc tgggcgagga
     2341 ccccgtgtac ctgatccagg actaccacct ggccctggtc ccccggttcc tgcgggagct
     2401 ccgtcccaac gccttgatcg cgcacttctc ccacacggcg atcgccgggg cttcttacct
     2461 gcgtgtcctc ccagggaaga tccgggaggg cctcttgcgc gggatgctgg gggcggacgt
     2521 cctgggcttc cattcccagg cctgggccga gaacttcctg atgtcgatcc ggctgctctc
     2581 gggcgcccgc gccgacctgg ctcggtcacg ggtgacgatc gagggccggg aggtggcgat
     2641 ccggacccac cccatctctg tggacgcggc cccgatgcgc gagctggcca ccacccgtga
     2701 gatccgggcg cggcgccgtg agctgaaccg ctggaggggg aacgcgcggc tgatcctccg
     2761 ggtggaccgt ctggagctca cgaagaacat cgtgcggggc ttccaggccc atgaggtgtt
     2821 cctccggcgc aatccccact ggcggggccg ggtgcggttc ctggccctgc tctcgccctc
     2881 ccggatagag ctggaggagt accgggcgta cggggaggaa tgcgtggccg aggcggaacg
     2941 gatcaaccgg gagctgggag acctggaatg gcggcccatc gaggtccagg tgcgcgagga
     3001 cttcccggga gccgtggccg cctacggcct gtacgacgtg ctgatggtga acccgatcta
     3061 cgacggcatg aacctggtct cgatggaggg gcccctgctg aaccggcgtc acggtgtcct
     3121 gatcctctcc cgcaacgcag gcgcctactg gaggctagga cgctacgcgg tggcggtgaa
     3181 cccgttcgac ctgcacgagc aggcggaggc catcgaggag gcactggaga tgcccgcgga
     3241 cgagcgggcc cggcgggctc ggggcctgtc gcggctggtc ctgtcgaacc ccccggcccg
     3301 atgggtcagg agccagctcg aggatctgga acgagcgcgg gtgcgccgag cccggaccgc
     3361 ctgatcgcgg tcctcacttc tgcagatccg cgaaatcggt gcccaggtag acggccacct
     3421 ggacgttctt cttgacgccg gtggccgagg ggtccaggcg ggagatctgg gggtccacgc
     3481 tcttgaagta caggcgcacc atctcctcgg cctcgatctt gtccgggggg gaccgataga
     3541 agacctcggt ctcggaggcg gtcgaggggg catcggccac ctggaccgcc ttgtatccga
     3601 acttccgctg gagcaggagc gccgtgtcgc cggccaggcc gttgacgccg gccgcattga
     3661 acacggcgat cttcacgccg acgaccttgg gggtcggcgg aggcttggag gaggacttcc
     3721 cgtcaccggg actggcagga gggctcgtcg gaacggtgga accgcccccg cctggagcgg
     3781 cgatcgtgcc gcccgaaggg aaggcctggt tcaggagcac cacgcccccc accacgaggg
     3841 ccaggacgat cacgaaccgc aagatgctgg tacccacgga gacgtaaaag ctcctgttcg
     3901 tcggtggctc gtgcctgccg aacatcctac ccttcgctcc ccgcggcccc aagcgtaggg
     3961 ttgccttcgg gctggccgag gacgctccct gcccggtcca cgatcaggcg gtcaacgagc
     4021 ggatctgacg gacggcgacc gtcatggcgg cgaggtcgcc cacgtcctcg agagccaggg
     4081 cccgcatgaa ccgggccagc cgcgccacga cctcggtgcg cgcgtcggcg aagcgcgcga
     4141 tggcctgctc gatgggacca tcgtccgcct cggacaggac gcgctcggcc acctgcctgc
     4201 ggacgagcaa caggtcgtcc tccatcgctt ggaccgccca ccgctgccac cggctggtgg
     4261 cccgcaggtc gctcagacgg ccttccagcc aatcgatgta gagggcctcg cccacggcga
     4321 agaaggctcg ggcggtatcc tccaccgagc gccccgtggc cgaggcgacc acgatcacgt
     4381 tcggtccgtg gaccagcaac ggctgcagga cgtgacgccg ggccagcgtt tgggggaccc
     4441 cctggtccgt cagccggtgc gcgatccgct cgcgatcctc gctccacccc cccggcacga
     4501 cccgctccac cacgtcgatg aaccgccgga acggctcgcg gtgggcctcg atcgcgcttc
     4561 cgagcttccc ggggaggtgc gccaggtacc accgtgcggt ggtctcgacc atccggtcca
     4621 cgcccggcat cagctcgccg aggatcgccg gttccaccga gcccaccagg gcctcgatcg
     4681 cctcccaccg ctccacggca tcggtcacat ccctggcgat gcgataggcg cgcaccacgt
     4741 ccgagggttc cgcgccggtc tcggtgacca atcgggagac gaacgtgatg ccctgggagt
     4801 tcaccacgtc gttggcgacg atcgtggaga ccagctcccg tcgtaggggg tgctcggaca
     4861 gcaggtcgcc gaagcgctcc accaccgcgg gggggaagta cccgcgcaga tcggcggcca
     4921 ggtactcgga atcgggcaac ggggagtcca gcagggcgtt cgtgagcgcg atcttcgcgt
     4981 aggcgagcag caccgccagc tccggacgag ccatgccgat gccggcgcgc cgccgctcgg
     5041 ccatctcatc cgtcctgggg agcgcctcca gggctcgatc caggtgccct ccgtcctcca
     5101 gcgaccgcat caggtcctcg tacgcctcca tccgcgagga ggaggggtct gcctcctggg
     5161 agaggatctg agcctggagg aagttgtcgt acaggacgtg gcgcaccaca tcctgctcca
     5221 cctggtggag gaggtggtcg cgctcctcga gcgtgagcgc gccccgctgg accgcgatgc
     5281 ccaggaggat
//
LOCUS       DASQQQ010000183         4360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_70155, whole genome shotgun sequence.
ACCESSION   DASQQQ010000183 DASQQQ010000000
VERSION     DASQQQ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70155"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..462
                     /gene="rplO"
                     /locus_tag="VE646_07150"
     CDS             16..462
                     /gene="rplO"
                     /locus_tag="VE646_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018177802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HZD79801.1"
                     /translation="MKLHHLKPAAGAKKDRRRVGRGRAGTGGKTAGRGTKGWGARHNP
                     KLGFEGGQMPLQRRLPKLKGFTNINRVEYAVVNVETLAKISEAGESGPIDPALLYRHG
                     LVRKGRPVKVLARGELDRALTVRAQAFSRAARSKIEQAGGTAEVMG"
     gene            482..1759
                     /gene="secY"
                     /locus_tag="VE646_07155"
     CDS             482..1759
                     /gene="secY"
                     /locus_tag="VE646_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZD79802.1"
                     /translation="MLRGFANAFKVPDLRKKILFTLFIVAIYRFGSFVPVPVVDIKGL
                     QQQAGQGFLSFIDLFSGGALTQMAVFGLGIMPYITSSIIMQLLTVVIPKLEQWQKEGE
                     TGIKKINQWTRYITVVLALLQSTGLVFLFHSGSSSLGGVNIFPTGTFTPPNIALIVLI
                     MTAGTAMIMWLGELITQRGIGNGMSVIIFIAVISRLPSEGHAILLQGGMGKFVTVLLI
                     GMLIVLAVVYVEQGQRRIPVQYAKRVVGRRMTTGGSTYIPLKVNQAGVIPIIFAASIL
                     YFPTLMASVYHASWFQTFVSQYVTNQRSFVYMTIYGLLVVFFAYFYTAITFNPVDTAD
                     NLRKYGGFVPGIRPGPPTANYLNDVLVRITLPGSLFLAAIALLPSIVLAFWVIQQFPF
                     GGTTLLITVGVALETMKQLESQLLMRHYEGFLR"
     gene            1770..2432
                     /locus_tag="VE646_07160"
     CDS             1770..2432
                     /locus_tag="VE646_07160"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013683766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HZD79803.1"
                     /translation="MRIVLLGPPGAGKGTQARRLADRYGLPIVATGDLFRDQVARETE
                     LGGRAKGYMDRGELVPDDLVTEMVMERLHEPDADEGFILDGYPRTVPQATSLEVALAA
                     EHRPLSAVLNFKIGDEMAVKRLTGRLVCPNCKRSYNLSFKPPRIEGSCDVCGHQLKLR
                     DDDDEATIRRRLEVYREQTAPLVLYFWERGILRQIDSEDPEEVVFDRTIEAIADLTEF
                     QT"
     gene            2429..3181
                     /gene="map"
                     /locus_tag="VE646_07165"
     CDS             2429..3181
                     /gene="map"
                     /locus_tag="VE646_07165"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZD79804.1"
                     /translation="MIIYKSPQEIERMRKAGRIVAGTIDTVLRAVRPGVSTGELDAVA
                     EGYIRERGATPSFKGYRGFPASICTSINQEVVHGIPSNERVVREGDILSLDFGAIWEG
                     FHGDSAVTVFVGEPPSSEAEKLVRITKEALEAGIAQIRRGGRLSDISHAVEQVVLGAG
                     FEVVREYVGHGIGRSLHEDPQIPNYGQPGRGPELRPGLVIAVEPMVNMGDWRTRVLAD
                     DWTVVTHDGSLSAHFEHTIAITDDGPEVLTAR"
     gene            3344..>4360
                     /locus_tag="VE646_07170"
     CDS             3344..>4360
                     /locus_tag="VE646_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZD79805.1"
                     /translation="MSTEPVTVLVGGRDQLERELVRFFMEEAGFRVLSMVSQDVDLVR
                     QVEVDRPDAVIVFSSLTDADGTDLVHRIRRASRRPKIVVVTPSPETAWKGPARGADAF
                     VEQWIGIQQLGSLLSRLCRRGPSEEPVSEEPAEAPGELARAGSGGGGGRALPPPPPPD
                     GGGRWDRWFARLQGAAVASVVFLALFVGGTIFNQSPPTPQRMPPAVAAHLNRAYVLLD
                     HLIDGMRDNIPSRIIVRYARQLSDERNSVAQAGDYTSALDQRIEQQVSPYLDDVPPAV
                     AQQVMTALGDLVAPPSPSPSPDPGVIALPTTSPSPTPDLSPSPTTAPSPTDAPSPTTA
                     PSPST"
BASE COUNT          682 a         1475 c         1523 g          680 t
ORIGIN      
        1 ggagggggag gaccggtgaa gctccaccac ctgaagccgg ccgccggggc gaagaaggac
       61 cgccgccgcg tgggccgcgg tcgggccggg accggaggca agacggccgg ccgcggcacc
      121 aagggctggg gcgcccggca caacccgaag ctcggcttcg agggcggcca gatgcccctg
      181 cagcggcgcc tgcccaagct gaaggggttc acgaacatca accgcgtgga gtacgcggtg
      241 gtgaacgtgg agaccctggc caagatctcc gaggccggcg agtcgggccc catcgacccc
      301 gcgctcctgt accggcacgg cctggttcgc aagggccggc cggtcaaggt gctggcccga
      361 ggggagctgg accgcgccct caccgttcgg gcgcaggcct tctcacgggc cgctcggagc
      421 aagatcgagc aggccggggg gacggcggag gttatgggct gaccaggtag gataggtccc
      481 ggtgctcaga gggttcgcca acgcgttcaa ggtccccgat cttcgcaaga agatcctgtt
      541 caccctgttc atcgtggcga tctaccggtt cggctccttc gtgccggtgc cggtggtgga
      601 catcaagggc ctccagcagc aggccgggca ggggttcctg tccttcatcg acctgttctc
      661 cggcggcgcg ctgacccaga tggcggtgtt cgggctgggc atcatgccct acatcaccag
      721 ctccatcatc atgcagctgc tcaccgtggt gatccctaag ctcgagcagt ggcagaagga
      781 gggcgagacc gggatcaaga agatcaacca gtggacccgc tacatcacgg tcgtgctggc
      841 cctgctgcag tccaccgggc tggtcttcct gttccactcg ggcagctcct cgctcggcgg
      901 cgtgaacatc ttccccaccg gcaccttcac cccgccgaac atcgcgttga tcgtgctgat
      961 catgaccgcc ggcaccgcca tgatcatgtg gctgggcgag ctcatcaccc agcgcgggat
     1021 cggcaacggg atgtcggtga tcatcttcat cgcggtgatc tcgcggctgc cgtcggaggg
     1081 ccacgcgatc ctgctgcagg gcggcatggg caagttcgtc acggtgctgc tgatcgggat
     1141 gctgatcgtg ctggccgtgg tctacgtgga gcaggggcag cggcggatcc ccgtgcagta
     1201 cgccaagcgg gtggtgggcc ggcggatgac caccggcggc agcacctaca tcccgctgaa
     1261 ggtcaaccag gccggcgtga tccccatcat cttcgccgcc tcgatcctgt acttccccac
     1321 gttgatggcc agcgtctacc acgccagctg gttccagacg ttcgtgagcc agtacgtcac
     1381 gaaccagcgg agcttcgtgt acatgacgat ctacggcctg ctggtggtgt tcttcgccta
     1441 cttctacacg gccatcacct tcaacccggt ggacacggcg gacaacctgc gcaagtacgg
     1501 gggattcgtg ccgggcatcc ggcccgggcc gcccaccgcc aactacctga acgacgtgct
     1561 ggtccggatc accctgccgg ggtcgctgtt cctggcggcc atcgccctgc tgccgtcgat
     1621 cgtgctggcc ttctgggtga tccagcagtt ccccttcggc gggaccacgc tgctgatcac
     1681 cgtgggcgtc gcgctggaga ccatgaagca gctggaatcc cagctgctca tgcgtcacta
     1741 cgagggcttc ctgaggtagg ggatcaccca tgcggatcgt gctcctgggg ccgccgggcg
     1801 caggcaaagg gacccaggcc cggaggttgg ccgaccgcta cggtctgccc atcgtggcca
     1861 ccggcgacct gttccgcgac caggtggccc gcgagaccga gctgggcggg agggccaagg
     1921 gctacatgga tcggggcgag ctggtgcccg acgacctggt caccgagatg gtgatggagc
     1981 ggctgcacga gccggacgcg gacgaggggt tcatcctcga cggctacccg cgcacggtgc
     2041 cccaggccac ctcgctggag gtggccctgg ccgccgagca ccggccgctg tcggccgtgc
     2101 tgaacttcaa gatcggcgac gagatggcgg tcaagcgcct caccggacgg ctggtctgcc
     2161 ccaactgcaa gcgctcgtac aacctcagct tcaagccgcc gcggatcgag ggctcctgcg
     2221 acgtctgcgg tcaccagctg aagctccggg acgacgacga cgaggccacg atccgccggc
     2281 gcctggaggt gtaccgggag cagaccgccc cgctggtcct gtacttctgg gagcggggca
     2341 tcctccgtca gatcgactcc gaggatccgg aggaggtcgt gttcgaccgc acgatcgagg
     2401 ccatcgccga cctcaccgag ttccagacat gatcatctac aagtcccccc aggagatcga
     2461 gcggatgcgc aaggccggcc ggatcgtggc cggcaccatc gacaccgtgc tgcgggcggt
     2521 gcgtcccggc gtgagcaccg gcgagctcga tgccgtcgcc gaggggtaca tccgtgagcg
     2581 gggcgccacg ccgtccttca agggctaccg ggggttcccg gcgtcgatct gcacctcgat
     2641 caaccaggag gtggtccacg gcatcccctc gaacgagcgg gtggtgcgcg agggcgacat
     2701 cctctcgctg gacttcggcg cgatctggga gggtttccac ggggactccg cggtcacggt
     2761 gttcgtgggg gagccaccgt cctccgaggc cgagaagctg gtccggatca ccaaggaggc
     2821 actggaggcc gggatcgcgc agatccggcg gggcgggcgc ctgtcggaca tctcgcacgc
     2881 cgtggagcag gtggtgctgg gcgcggggtt cgaggtggtc cgcgagtacg tggggcacgg
     2941 gatcggccgc agcctgcacg aggacccgca gatcccgaac tacggacagc ccggacgcgg
     3001 gccggagctg aggcccggcc tggtgatcgc cgtcgagccc atggtgaaca tgggggactg
     3061 gcggacccgg gtgctggccg acgactggac cgtggtcacg cacgacgggt cgctctccgc
     3121 gcatttcgag cacacgatcg ccatcaccga cgacggtccc gaggtgctca ccgcgcggta
     3181 gtggaggcgc tgcgcggcaa agcgacgaag cgcacgtaca tcaagtggac ctttcggacc
     3241 ccccacctac gagataacgt tatgcatgca ggtccgaagc atgagggatc gtcggctccg
     3301 gaggtcgctg ggtggggcgg tggagccggg gaggactgga tcgatgagca cggaaccggt
     3361 caccgtcctg gtggggggac gcgaccagct cgagcgggag ctggtccgct tcttcatgga
     3421 ggaagcgggc ttccgcgtgc tctccatggt ctcccaggac gtcgatctgg tccgccaggt
     3481 cgaggtggac cggcccgatg cggtgatcgt gttctcgagc ctgaccgacg cggatgggac
     3541 cgacctggtg caccggatcc gtcgggcctc gcggcggccg aagatcgtgg tcgtgacccc
     3601 ctcacccgag acggcctgga agggccccgc tcggggagcg gatgccttcg tggagcagtg
     3661 gatcgggatc cagcagctgg gttcgctcct gtcccggttg tgccgccgtg ggccgtccga
     3721 ggagccggtc tccgaggagc cggccgaggc tccaggggag ctggcacgcg ccggctccgg
     3781 cggtggaggc gggagggcgc tccctccgcc gccaccaccg gacggtgggg ggcgctggga
     3841 ccgatggttc gcgcgcctgc agggcgcggc cgtggcctcg gtcgtcttcc tggccctgtt
     3901 cgtgggcggg acgatcttca accagtcgcc gcccacgccc cagcggatgc cgccggccgt
     3961 ggcggcccat ctgaaccgtg cgtacgtcct gctcgatcac ctgatcgacg ggatgcgcga
     4021 caacatccct tccaggatca tcgtccggta cgcccgtcag ttgtccgacg aacggaactc
     4081 cgtggcccag gccggtgact acacctcggc gctggaccaa cggatcgagc agcaggtctc
     4141 gccgtacctg gacgacgtgc ctccggccgt ggcccagcag gtgatgaccg ccctgggcga
     4201 tctggtggca cccccgtcgc cgtccccctc tccggatccg ggggtcatcg ccctccccac
     4261 gacgagcccc tcgccgaccc cggacctcag ccccagcccc accaccgctc cgagccccac
     4321 ggatgctccc agccccacca ccgctcccag cccaagcacg
//
LOCUS       DASQQQ010000184        14130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_398254, whole genome shotgun sequence.
ACCESSION   DASQQQ010000184 DASQQQ010000000
VERSION     DASQQQ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14130
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398254"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..678
                     /locus_tag="VE646_07175"
     CDS             166..678
                     /locus_tag="VE646_07175"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019927122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HZD79806.1"
                     /translation="MPRIELIEGDITEQQVDAIVNAANSSLLGGGGVDGAIHRRGGPQ
                     ILAECRQIRRTAFRDGLPTGRAVATTAGRLPARWVIHTVGPVYSRSEDRSALLASCHT
                     ESLRVADELDARTVAFPAISTGVYGYPLELAAPVAIRAVREADTRVEEVRFVLFGQGA
                     YRAFDRALRA"
     gene            738..1082
                     /locus_tag="VE646_07180"
     CDS             738..1082
                     /locus_tag="VE646_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZD79807.1"
                     /translation="MDELSTDLTTPKGVHEKLDRIQLVDCREPYEWNAGRIEGARHLP
                     LNSIMAGVTGDLETERPVVVVCRSGNRSELATLMLQARGFEAYNLEGGMEEWAREGLP
                     FSTPDGRPGHVA"
     gene            1173..2636
                     /locus_tag="VE646_07185"
     CDS             1173..2636
                     /locus_tag="VE646_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZD79808.1"
                     /translation="MADVEAARRELEEILGATEVVAEPLALRLFARDASMVEGGCALV
                     AYPHSPEEVSGCLRVAERHGLPVVPRGSGTGLAGGATPMGDALVVVTTKMDRILEIRP
                     VDRLAWVEPGVLNLDLANALAPHGFTYAPDPSSQQTCSIGGNVNTNAGGPHCLAYGVT
                     SNHILAVDLALPDGSRVRVGSEAPDWGGYDLRGVVVGSEGTLGIVVAACVRLTPLPPA
                     VRTMLLDFSTVEDCAGTVSAIIASGVVPAAVEMMDRGIVVAAEAFAHAGYPTDAAAIL
                     IVEVDGTQAAVAAQERAVREAAGANAVRSIRMAADEEERALLWKGRKSAFGAVARIAP
                     HYHLHDCVVPRTKLVEVLGGVYKIAERHQLTVTNVFHAGDGNLHPLLSFDRRVPGTLE
                     RVLAASEEIVRLCVDAGGALSGEHGIGLEKRDFMPLVFGEEDLRVQACVRAVFDPHGR
                     MNPHKVLPAGARCGDFAMARGSDAAQAAADLPEGAWI"
     gene            2627..3673
                     /locus_tag="VE646_07190"
     CDS             2627..3673
                     /locus_tag="VE646_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZD79809.1"
                     /translation="MDLIHPHTPGDVQDALREANAEGSTVLVVGGRTHMDLGNPTEVD
                     AELWTTQLDELIDHQPAEMIAVVEAGMRVGDLEAMLSETGQEWPVDAPAEATVGGVIA
                     AGASSPRRLKVGAVRDTVLQAEVVTGDGRVVRSGARTVKNVTGYDVHKLLAGSLGTLG
                     VIIQAALKLRPRPGAATTFRCPGDLELAAAVLDAVPGAAAVLVTSAGLEVRLEGWPEE
                     VQEQARAVRSVAGAVDEGDGPFPADRPWEDRPTVVQVAVPPSRLGRLVGPPLERWGAL
                     VGVGTAWIGARDAAELGEVRRRAAELGGIAPAIKGPGGLGESAPPAAELQRRIKAALD
                     PGRVLAPGRFWGGI"
     gene            3673..4998
                     /locus_tag="VE646_07195"
     CDS             3673..4998
                     /locus_tag="VE646_07195"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HZD79810.1"
                     /translation="MLGAPDDVGGAIGTDLPEGTRFVGPVAGLGPNWGPGDAPAEGDL
                     AACVACGLCLPHCPTYRLSGEETASPRGRIAAMRAVAEGQARIDPAFLGYMDACLSCR
                     ACEDVCPSHVPFGRMMERARVQAEPLRSRRARMLRWLGLDVALPHRRLLGAARALTPV
                     ARPFLGKRVRALMPRGTRPGPRLPTVTETEGEPRGTVALLTGCVQDQWFRHVNRASAR
                     VLSRAGWRVLAPRGQVCCGALQAHNGRLGTARELAAQNLAVLGSADHVVVNAAGCSAH
                     MKDYGDLLGTPEARRFSSRVRDLMEFLDEHGTAATGHLPPEEGPIAYHDPCHALRAQG
                     IRAQPRRLLDAIDGVELLEIADGGRCCGAAGIYELTQPLTADALMHQKAQAVAATGAR
                     VVASANPGCSMQLDAGLRAIGADIRVVHPVELIDAAYGASERMRRGTAG"
     gene            5061..5717
                     /locus_tag="VE646_07200"
     CDS             5061..5717
                     /locus_tag="VE646_07200"
                     /inference="COORDINATES: protein motif:HMM:NF039195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HZD79811.1"
                     /translation="MSRLVGIIAGAALLFAAAACSSASGSGTTPSTPIATPSATIGAS
                     PSGVLPTGSAPPSVIPSGTMQTYSSHGVTFQYPADWQGMTPSGTATVFGNQAWSTTVG
                     TDRTNFVTVASYRINASVTQGNIQQQMQGIASQLTGLFQQAGGSLKSGPTVETVAGLP
                     ALSFVGTATPNGTQVTNHVVLIFHQSTEYLVDCQYTSSNSSQMEQGCSTILDSFQVTG
                     "
     gene            complement(5789..6169)
                     /locus_tag="VE646_07205"
     CDS             complement(5789..6169)
                     /locus_tag="VE646_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HZD79812.1"
                     /translation="MATDLTIVTEDRPGELARVGEALGGAGINIEAMAGFGVEGRGVI
                     HVLVEDAGSARSALEASGLTVSEESEALVMPVDADVDRPGALGEIARKVADAGINFRA
                     VYLGTKNRAVAVTEDNERVLSLLS"
     gene            complement(6244..7821)
                     /locus_tag="VE646_07210"
     CDS             complement(6244..7821)
                     /locus_tag="VE646_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-coumarate--CoA ligase family protein"
                     /protein_id="HZD79813.1"
                     /translation="MIVRSPYPDVEIPDVSLPAYVLERAAELGDKPALIDGPTGRTLR
                     YGELVTAVRRAAGGLAARGLAKGEVVGIFAPNVPEWAIVFHAVGRLGGVNTTVNSLGT
                     EQDLAEQLQDAGARFLVTVPPFLDRAVPAAAKAGVEEVFVLGEAEGATPFSSLLARDA
                     EPPTVDIDPDRDLVALPYSSGTTGLPKGVMLTHRNLVANVCQTEAVQRIEPSDTLIGV
                     LPFFHIYGMTVVLNLALRRGATVVTMPRFELEPFLGLLHEHRVTRGYLVPPIVLALAK
                     HPAVEGYDLSSLHVIMSGAAPLDAELAAACAERVGCRVVQGYGLTETSPVTHASGDRE
                     GEGRPGSIGPPVPNTECRVVDWGTGEDQEVGRDGELCIRGPQVMIGYLNSPSATANTI
                     DPDGWLHTGDVGHVDQDGYFYVVDRLKELIKYKGYQVPPAELEAVLIGHPAVTDAAVI
                     PSADPEAGEVPKAFVVARAEVSAEELMAFVAERVAPYKRVRKVEFVEQIPKSASGKIL
                     RRMLVEQERAGTEPSAG"
     gene            complement(7911..9077)
                     /locus_tag="VE646_07215"
     CDS             complement(7911..9077)
                     /locus_tag="VE646_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZD79814.1"
                     /translation="MTALRPSRKASSFTESVIREMTRLAIAHDAINLSQGYPDFPAPM
                     ELKEAARQAIAEDINQYPITWGSFAFLEAIADKYRREYGMEVDPETMVCVTCGSTEAM
                     IASMLGLLDPGDEVVLFSPFYENYCPDAILAGATPRYVTLSPPGWSFDREELAAAFTD
                     RTRAIVINTPNNPTGKVFTREELEVIADLCLRHDVVVLTDEIYERILYDGHRHVPPAT
                     VQGLEDRTVTISALSKTYAVTGWRVGWTVAPPQLTAGIRAVHDFLTVAAAAPLQEAGV
                     VALSLPEDFYRQQVQEYRDRRDLLMRILAETGFKAEAPQGAYYVMADVSHLGFADDVE
                     AATRLTEDVGVAVVPGSSFFSRPELGRHLVRFAFCKRLETLEEAGRRLRAFLAV"
     gene            complement(9195..10082)
                     /locus_tag="VE646_07220"
     CDS             complement(9195..10082)
                     /locus_tag="VE646_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79815.1"
                     /translation="MREDVKDLLERATASFEPTPVEPEEVVRLEQRRQRRTRIGTAFV
                     ALAVFAAAGLLTWNAFAPRRPLQPGGGDFESGSYVMDHIKVTYPYVSPRADADPGFAG
                     VEFRSTWTHDTFPGDHNCTVTAYDAAGMVGETRIELSSLSRVAVWDHPVAVPVTSEPT
                     VASVTCDAQRLDTPVAFVVSDPRVEPVTEGGRVSGFRYSFRVDAPPNVRPPEYVGTNV
                     CVVLLRTSTGKELFHGRLTVTTLAPHTVSSKILRELAGNVDTRLTLNVSCEPFTGTGV
                     DAVSKGGQYVSNPAAHSAS"
     gene            complement(10075..10575)
                     /locus_tag="VE646_07225"
     CDS             complement(10075..10575)
                     /locus_tag="VE646_07225"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZD79816.1"
                     /translation="MRVMIETRGAAEDFSVFFRREYPRLARACLLLTSHHGDAEELAQ
                     EALARAYMKWADVAIMESPAGYVYRTALNLNRKRLRRLAVRARHVLTPTPHLDVDDAD
                     ERVDLMRAIRALPRGQREALVMVEWLDLDVEEAASLLGIAPASVRGRLFRARQALRER
                     FGGTDA"
     gene            complement(10709..11086)
                     /locus_tag="VE646_07230"
     CDS             complement(10709..11086)
                     /locus_tag="VE646_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZD79817.1"
                     /translation="MPKLCPSAKASSFTESVIREMTRLAIAHDAINLSQGYPDFPAPM
                     EVKEAARAAIADDINQYPITWGSFAFLEAIAAKYRREYGMEVDPETMVCVTCGSTEAM
                     IASLLGILDRGDEVVLFEPYYEN"
     gene            complement(11858..13018)
                     /locus_tag="VE646_07235"
     CDS             complement(11858..13018)
                     /locus_tag="VE646_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZD79818.1"
                     /translation="MATAEIDPLGRLTGFGRELRHRGLAVGTGRIVTFCRAVAALSPP
                     SRNDLYWAGRASLVGRPEDVEAYDRAFESYFRDGVRIELDLPGADAAAAAQEQVQQGA
                     EDPSVEGPALAAEWRSAAEGEEPEGEAAVRLVASAVEVLREKSFADLTDEERERVARL
                     IRRIAVSVPVRRTRRLHPAATGPRFDVKRTLRRSLRTQGEPFQRAWRRRGQRVRPLVL
                     ILDISGSMAPYARALVQFAFAAMAVGRTVEVFCFGTRLTRVTRTLRTKDPDRALHEIG
                     RMVADWEGGTRIGISLKELLDGWSQRAALRGSVAVICSDGLERGDADLLRDQMARLHR
                     LAHRVVWVNPLKGSPRYEPLARGMAAALPSVDVFLPGHNLESLEELSRVLAG"
     gene            complement(13005..13886)
                     /locus_tag="VE646_07240"
     CDS             complement(13005..13886)
                     /locus_tag="VE646_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZD79819.1"
                     /translation="MGSLPYASVEELRSALRGRRYLADRGLATAIYLAASLDKPLLLE
                     GEAGVGKTEVAKVLADLLDRELIRLQCYEGIDASQALYEWNYSRQLIAVRALQDVTVE
                     AHVADLFGPQFLVERPLLAAIRAGSRAVLLVDELDRADDEFEAFLLEILSEFAVTIPE
                     IGRIGATEPPMVVVTSNRTRELHDALKRRCLYHWIDHPALDREIEIVRARAPEISEAL
                     ARDVASAVMRLRSFDIAKRPGVAETIDWANAVSFLGVDRLDEPTVEATLGTVLKDHED
                     QELARSRIAEVIGDGDS"
BASE COUNT         2143 a         5055 c         4906 g         2026 t
ORIGIN      
        1 ggccgggcag ggcgtgctcg agcgctccac cgatcccgga cggcgcgagg ggtcggtctc
       61 cctgcgtccg cttcggctcg gtgcccttga gggagcggtg ctccgctgcc caccgcggtg
      121 aggtggtgct cggcccttgg accggtcacc tcctacgatg gggaggtgcc gaggatcgag
      181 ctgatcgagg gggacatcac cgagcagcag gtcgatgcca tcgtcaacgc ggccaactcc
      241 tccttgctcg ggggcggcgg tgtggacggt gcgatccacc gacggggcgg cccgcagatc
      301 ctggccgaat gccgtcagat ccgccggacc gcgttcaggg acgggctgcc gacgggccgg
      361 gcggtggcca ccacggccgg gcggctcccg gcccgatggg tgatccacac ggtgggcccg
      421 gtctactcgc ggtccgagga ccgctcggcg ctgctcgctt cgtgccacac ggagtcgctc
      481 cgggtggccg acgagctcga cgcgcggacc gtggccttcc ccgccatctc gaccggcgtg
      541 tacgggtacc cgctcgagct ggcggccccg gtcgccatcc gagccgtgcg cgaggcggac
      601 acgcgcgtgg aggaggtccg cttcgtgctg ttcgggcagg gggcctatcg agcgttcgat
      661 cgcgccctcc gagcatgatc tacccccagg catgaccccg ggcataatcc actccgctgg
      721 tagcattttt aggtgccatg gatgagctga gcaccgacct caccaccccc aagggcgtgc
      781 acgagaagct ggatcggatc cagctcgtgg actgccgcga gccctacgag tggaacgccg
      841 gccggatcga aggagcccgg caccttccgc tcaactcgat catggccggg gtgaccgggg
      901 acctggagac ggagcgaccc gtggtggtcg tgtgccgctc ggggaaccgc agcgagctcg
      961 cgaccctgat gctccaggcc cgcggcttcg aggcctacaa cctcgagggc ggcatggagg
     1021 agtgggcccg ggagggtctg cccttctcca ctcccgacgg gcgcccgggc cacgtggcct
     1081 gagagaccct ccttccctcg cgcccgttcc tccgggaagc ggccaggcgt gggaaaccga
     1141 ccgcccggcg cgttagcctg aagccggcag tcatggccga cgtcgaagca gcccgtcgcg
     1201 agctggagga gatcctcggc gccaccgagg tggtggccga gcccctcgct ctccgtctgt
     1261 tcgcgcgcga cgcgtcgatg gtcgagggcg gctgtgccct ggtcgcctac ccgcactcgc
     1321 ccgaggaggt gagcggctgc cttcgcgtgg ccgaacggca cgggctgccg gtggtgcccc
     1381 gggggtcggg gaccggcctg gccggggggg ccacgcccat gggggacgcc ctggtggtgg
     1441 tcacgaccaa gatggaccgg atcctcgaga tccggcccgt cgaccgacta gcctgggtgg
     1501 agccgggcgt cctcaatctc gacctggcca acgccctggc gccccacggg ttcacctacg
     1561 cgcccgaccc ctcctcgcag cagacctgct cgatcggcgg caacgtgaac acgaacgcag
     1621 gcggccccca ctgcctcgcg tacggcgtga cctccaacca catcctcgcg gtggacctgg
     1681 cgctccccga cggctcgcgg gtccgggtgg gctccgaggc accggactgg ggcggctacg
     1741 acctccgcgg cgtggtggtg ggctcggagg gcacgctcgg catcgtggtc gccgcttgcg
     1801 tccgccttac gccgctgcca ccggccgtcc gcaccatgct gctcgacttc tccaccgtgg
     1861 aggactgcgc gggcaccgtg tcggccatca tcgcgagcgg ggtcgtgccg gcggccgtgg
     1921 agatgatgga tcggggcatc gtggtggccg cggaggcgtt cgcccacgcc ggctatccca
     1981 cggacgccgc ggccatcctg atcgtggagg tggacggtac gcaggcggcg gtggccgcac
     2041 aggagcgcgc ggtgcgcgag gcggccggcg cgaacgcggt ccgcagcatc cggatggcgg
     2101 ccgacgagga ggagcgggcg ttgctgtgga aggggcgcaa gagcgcgttc ggcgcggtgg
     2161 ctcgcatcgc gccccactac cacctccacg actgcgtggt gccgcgcacc aagctcgtgg
     2221 aggtcctggg cggcgtctac aagatcgcgg agcggcacca gctcacggtg acgaacgtgt
     2281 tccacgcggg ggacgggaac ctgcatccct tgctgtcctt cgaccgtcgg gtgccgggca
     2341 cgctggaacg ggtgctggca gcgtcggagg agatcgtgcg gctgtgcgtg gatgcgggcg
     2401 gcgccctgtc cggcgagcac gggatcggcc tggagaagcg cgacttcatg cccctggtct
     2461 tcggcgagga ggacctccgg gtgcaggcgt gcgtccgagc cgtgttcgac ccgcacggcc
     2521 gcatgaaccc gcacaaggtc ctgccggccg gcgcgaggtg cggggacttc gccatggccc
     2581 gcggctccga tgcggcgcag gcggccgccg atctgccgga gggcgcatgg atctgatcca
     2641 cccgcacacc cccggcgacg tgcaggatgc gcttcgtgag gcgaacgccg aggggagcac
     2701 ggtgctggtc gtcggcggac ggacccacat ggacctgggg aacccgacgg aggtcgatgc
     2761 cgagctgtgg accacgcagc tcgacgagct gatcgatcac cagcccgccg agatgatcgc
     2821 ggtggtcgag gccgggatgc gcgtgggcga cctggaggcg atgctgagcg agaccgggca
     2881 ggagtggcca gtggacgccc cagccgaagc caccgttggc ggcgtgatcg ccgccggagc
     2941 ctcctcgccg cgacggctga aggtgggcgc cgtgcgcgac accgtgctcc aggccgaggt
     3001 ggtgaccggc gatgggcggg tcgtccgcag cggggctcgg acggtcaaga acgtcaccgg
     3061 gtacgacgtc cacaagctgt tggcggggtc cctcgggacg ctcggcgtga tcatccaggc
     3121 agccctcaag ctccggccgc gccccggcgc cgccaccacc ttccggtgcc ccggcgatct
     3181 ggagctcgcc gccgccgtcc tcgatgcggt gccgggcgcc gcagcggtgc tggtcacctc
     3241 cgcggggctc gaggtccgcc tggagggatg gccggaggag gtccaagagc aggcccgagc
     3301 cgtgcgctcc gtggccgggg cggtggacga gggcgacggc cccttccccg ccgaccggcc
     3361 ctgggaggac cgacccacgg tggtgcaggt cgccgtcccg ccgtcccgcc tgggccggct
     3421 ggtcggcccc ccgctcgagc gctggggggc gctcgtcggc gtggggacgg cgtggatcgg
     3481 cgcgcgggat gccgcggagc tcggggaggt ccgccgccgc gcggcggagc tcggcgggat
     3541 cgcgccggcg atcaaggggc cgggtggact cggcgagagc gcgccccccg cggccgagct
     3601 gcagcgacgg atcaaggcag cgctggaccc cggccgcgtc ctggcccccg gccggttctg
     3661 gggggggatc tgatgctcgg agcgccggac gacgtgggtg gggccatcgg gaccgacctc
     3721 cccgagggga cccggttcgt cggaccggtg gcggggctcg gaccgaactg gggcccgggc
     3781 gacgctccgg ccgaggggga cctggcggca tgcgtggcgt gcggactgtg cctgccccac
     3841 tgccccacgt accggctgag cggcgaggag accgcctcgc cgcgagggcg gatcgcagcg
     3901 atgcgggccg tggccgaggg gcaggcgcgg atcgaccccg ccttcctggg ctacatggac
     3961 gcctgcctgt cctgccgggc gtgcgaggac gtctgtccct cgcacgtccc cttcgggcgg
     4021 atgatggagc gagcgagggt ccaggccgag ccgctccggt cgcgccgagc gcggatgctc
     4081 agatggctgg gactggacgt ggcgctcccc catcggcgcc tgctcggtgc ggcgcgagcc
     4141 ctgacgcccg tggcccgccc cttcctgggc aagcgggtgc gagccctgat gcccaggggg
     4201 acccggcccg ggccacggct gccgacggtg acggagaccg agggcgaacc gaggggcacg
     4261 gtcgcgctcc tcaccgggtg cgtgcaggac cagtggttcc gccacgtgaa ccgggccagc
     4321 gcccgcgtgc tctcgcgagc aggatggcgg gtgctggccc ctcggggaca ggtctgctgc
     4381 ggggctctgc aggcgcacaa cgggcggctg gggacggctc gcgagctcgc cgcgcaaaac
     4441 ctggcggtcc tgggcagcgc ggaccacgtg gtcgtgaacg ccgccggatg cagcgcccac
     4501 atgaaggact acggcgacct actcggcacg ccggaggcac ggcgcttctc ctcccgggtc
     4561 cgggacctca tggagttcct cgacgaacac ggaaccgctg ccaccggtca cctgccgccg
     4621 gaggaaggtc cgatcgccta ccacgacccg tgtcacgcct tgcgggctca ggggatccgg
     4681 gctcaacccc gtcggctcct cgacgcgatc gacggcgtgg agctcctgga gatcgccgac
     4741 ggcgggaggt gctgcggggc ggccgggatc tacgagctga cccagccgct caccgcggat
     4801 gcgctgatgc accagaaggc gcaggccgtg gccgccacgg gcgcgcgggt cgtggcctct
     4861 gccaacccgg gatgctcgat gcagttggat gcgggcctcc gcgcgatcgg cgccgacatc
     4921 cgtgtggtgc acccggtcga gctgatcgac gcggcgtacg gggcgtccga acgcatgcga
     4981 cgcgggaccg ctgggtaggg gcggaagaag atcgaacgcc gagaacctgg gaggtctcca
     5041 tgcatgcccg cagacatcgc atgagccgac tggtgggcat catcgccggc gcggctctcc
     5101 tcttcgcggc cgccgcatgc agcagcgcga gcggctccgg aaccaccccc agcacgccga
     5161 tcgccacgcc cagcgcgacg atcggcgcat cgccgagcgg cgtgctgccc accggcagcg
     5221 cgcctccgtc cgtgatcccg agcggcacca tgcagacgta ctcgtcgcac ggcgtcacgt
     5281 tccagtaccc ggccgattgg cagggaatga ccccctccgg caccgctacc gtgttcggca
     5341 accaagcctg gagcacgacc gtggggaccg atcgcacgaa tttcgtgacg gtcgcgtcgt
     5401 accggatcaa cgcctcggtc acgcagggca acatccagca gcagatgcag ggcatcgcga
     5461 gccagctcac cggcttgttc cagcaggccg gcggctcgct gaagtccggt cccacggtcg
     5521 agacggtggc cggactcccc gcactgagct tcgtgggaac cgccaccccg aacgggacgc
     5581 aggtgaccaa ccacgtggtg ctgatcttcc atcagagcac cgagtacctg gtggattgcc
     5641 agtacacgag ctcgaactcc agccagatgg agcagggctg ctccaccatc ctggatagct
     5701 tccaggtcac cggctgatca tcgagcacgg tcggatcgca tcgacccggc cggcggtcgc
     5761 atcggaccgc cggccgggtt cggcgcgttc acgacagcag cgacaggacc ctctcgttgt
     5821 cctcggtcac ggccaccgca cggttcttcg tgccgaggta gacggcccgg aagttgatcc
     5881 cggcgtccgc caccttccgg gcgatctccc ccaacgcgcc ggggcggtcc acatccgcgt
     5941 ccaccggcat caccaaggcc tcgctctcct cggacacggt gaggccggac gcctccaggg
     6001 cggagcgggc cgagcccgcg tcctccacca ggacgtggat cacgccccgc ccctcgacac
     6061 cgaagccagc catggcctcg atgttgatcc cagccccgcc cagcgcctca ccaacccggg
     6121 ccagctcgcc cggacgatcc tcggtgacga tggtcagatc ggtcgccatc tgcgctcctc
     6181 ctcccttccc gtgcgagcgg tctacccgtt ccggccccaa cggtcaaccg ccggccggaa
     6241 cgctcaaccg gcgctcggtt cagtcccggc ccgctcctgc tccaccagca tccgccgtag
     6301 gatcttgccc gaggccgact tggggatctg ctccacgaac tccaccttcc gcacgcgctt
     6361 gtacggtgcg acccgctcgg ccacgaaggc catgagctcc tcggcgctca cctcggcccg
     6421 ggccacgacg aacgccttgg ggacctcccc ggcctccgga tccgcgctcg ggatcaccgc
     6481 cgcatccgtc accgccgggt ggccgatcag gacggcctcg agctccgccg gcggcacctg
     6541 gtaccccttg tacttgatga gctccttcag gcgatcgacc acgtagaagt agccgtcctg
     6601 gtccacgtga ccaacgtctc ccgtgtgtag ccacccatcc ggatcgatcg tgttcgcggt
     6661 ggcggacggg ctgttcaggt agccgatcat cacctgcgga ccgcggatgc acagctcgcc
     6721 gtcccgaccg acctcctgat cctcccccgt gccccagtcc accacccggc actcggtgtt
     6781 cgggacgggc ggaccgatcg acccgggtcg cccctctccc tcccggtctc cactggcatg
     6841 ggtgaccggg ctggtctcgg tcaggccgta gccctgcacg acgcggcacc ccacccgctc
     6901 ggcacaggcg gccgcgagct ccgcatcgag cggcgcggcc ccggacatga tcacgtgcag
     6961 cgaggacagg tcgtacccct ccacggctgg gtgcttggcc agggccagca cgatgggcgg
     7021 cactaggtag ccgcgggtca cgcggtgctc gtggagcaga cccaggaagg gctccagctc
     7081 gaacctcggc atcgtgacga cggtggcgcc tcgacgcagc gcgaggttca ggaccacggt
     7141 catcccgtag atgtggaaga aggggaggac gccgatcagc gtgtccgagg gctcgatgcg
     7201 ctggaccgcc tcagtctggc agacgttggc caccaggttg cggtgcgtga gcatgacccc
     7261 cttgggcagc ccggtggtcc cgctcgagta gggcagcgcc accagatccc ggtcgggatc
     7321 gatgtccacc gtgggcggct cggcgtcacg ggccagcagg gacgagaagg gcgtcgcccc
     7381 ctcggcctcc cccagcacga acacctcctc cacgcccgcc ttggccgccg ccggcacggc
     7441 ccggtccagg aagggcggga ccgtcaccag gaaccgagcg cccgcgtcct gcagctgctc
     7501 ggccaggtcc tgctccgtgc cgagcgagtt cacggtggtg ttcacccctc ccagccggcc
     7561 cacggcgtgg aacacgatcg cccactcggg cacgttcgga gcgaagatgc cgaccacctc
     7621 ccccttcgcg agcccccggg cggccaggcc cccggcggcg cgacggacgg cggtcacgag
     7681 ctccccgtag cggagcgtcc ggccggtggg accatcgatc agggccggct tgtcgccgag
     7741 ctccgcggcg cgctcgagca cgtaggccgg cagggacacg tcggggatct ccacgtccgg
     7801 gtacgggctc cgaacgatca tcggcggcct ccttcggtcc tggcgtgagg tccgaaccgt
     7861 accgctccct agggaagcct gcagcccgcc gccggtggga gtcctcgggc tcagacggcc
     7921 aggaacgcgc ggaggcggcg accggcctcc tcgagggtct ccagccgctt gcagaaggcg
     7981 aaccggacca gatggcgccc cagctcggga cgggagaaga aggaggagcc gggcaccacc
     8041 gccacgccca cgtcctccgt cagcctggtc gccgcctcca cgtcgtcggc gaacccgagg
     8101 tgggagacat ccgccatcac gtagtaggcc ccctgtgggg cctccgcctt gaaccccgtc
     8161 tccgccagga tcctcataag caggtcgcgc cggtcccggt actcctgcac ctgctgccga
     8221 tagaagtcct cgggcaagct cagggccacc acccccgcct cctgcagggg cgccgcggct
     8281 gccaccgtga ggaagtcgtg gacggcacgg atcccggccg tgagctgcgg cggagccacc
     8341 gtccagccca cccgccagcc ggtcacggca tacgtcttcg acaacgcgct gatcgtgacc
     8401 gtccggtcct ccaacccctg tacggtggcc ggcggcacat gccggtgccc gtcgtagagg
     8461 atccgctcgt agatctcgtc ggtgagaacg accacgtcgt gccggaggca gagatcggcg
     8521 atcacctcca gctcctcacg cgtgaacacc ttgccggtgg ggttgttcgg cgtgttgatc
     8581 acgatcgctc gcgtccggtc cgtgaacgca gccgccagct cctcccggtc gaagctccag
     8641 cccggtgggg acagggtcac gtaccgaggc gtggccccgg caaggatggc atcggggcag
     8701 tagttctcgt aaaaggggct aaaaaggacg acttcgtccc cgggatcgag gaggccgagc
     8761 atcgaggcga tcatcgcctc ggtcgagccg caggtgacgc agaccatcgt ctccgggtcg
     8821 acctccatgc cgtactcgcg ccggtacttg tccgcgatcg cctcgaggaa ggcgaacgag
     8881 ccccaggtga tcgggtactg gttgatgtcc tcggcgatgg cctgtcgggc ggcctccttg
     8941 agctccatcg gcgcggggaa gtcggggtag ccctgcgaca ggttgatcgc gtcgtgcgcg
     9001 atggccagcc gggtcatctc gcggatcacc gactcggtga acgaggaggc cttccgcgag
     9061 gggcgcagcg cggtcatcgt gccggatgat accggctcgt atagcctccc caccccttcg
     9121 ggtcggacaa gggacatcct ggctgggagc cagcccggaa cctgacatgt cgcttccgtc
     9181 acgaaacgac accctcacga tgccgagtgc gccgccgggt tggatacgta ctgaccaccc
     9241 ttcgagactg catccactcc tgtgcccgtg aacggttcgc aggacacgtt gagtgtgagt
     9301 ctcgtatcaa cgttgcctgc cagttcgcga aggatcttcg atgagacggt gtgaggggca
     9361 agggtcgtga cggtcagccg accgtggaag agctcctttc cggtcgacgt ccgaagcaac
     9421 accacgcaaa cgttggtgcc gacgtactcg ggcggacgta cgttcggcgg ggcatcgact
     9481 cggaacgagt accggaatcc gctgactcgg cccccctcgg tcaccggttc gaccctagga
     9541 tcggatacga cgaaggcgac tggcgtatcg aggcgttgtg cgtcgcaagt gaccgatgcc
     9601 actgtcggct cgctcgttac cggtacggcc acggggtgat cccacaccgc aacacgactc
     9661 agcgaactca actcgatccg cgtttcgccc accatcccag cggcgtcgta cgccgtgacc
     9721 gtgcagttgt gatccccggg aaaggtgtca tgcgtccagg tagaccggaa ctcaacacca
     9781 gcgaacccag ggtcggcatc ggcccgtggg ctgacgtagg gataggtcac cttgatgtga
     9841 tccatcacgt acgaaccgga ctcaaaatcg cccccaccgg gttgcagtgg cctgcgcggc
     9901 gcgaacgcgt tccaggtcag cagcccggcc gccgcgaaga cagcgagggc gacgaacgct
     9961 gtgccgatcc tggtcctacg ttggcgtcgc tgttcaagtc gcaccacctc ctcaggctcg
    10021 accggcgtcg gctcgaacga agccgtcgcc cgttccagca gatccttcac atcctcacgc
    10081 atcagttcct ccgaatcgtt cgcgaagcgc ctgccgtgcc cggaacagcc gtccgcgtac
    10141 cgatgcgggc gcgatcccga gcaacgaggc ggcttcctcc acatccaggt cgagccactc
    10201 gaccatgacg agtgcctctc gctgaccacg ggggagcgcg cggatcgcgc gcatcaggtc
    10261 gacccgttca tcggcgtcat caacatcgag atgcggtgtg ggcgtcagga cgtgtcgggc
    10321 ccgaacggcg agccggcgca gtcgcttgcg gttcaggttc agcgcggttc ggtacacgta
    10381 gcccgctgga gactccatga tcgcgacgtc tgcccacttc atgtaggccc gagctagcgc
    10441 ctcctgggcg agctcttcag catcgccatg gtgcgacgtg agcagcaggc aggcacgtgc
    10501 cagccggggg tattcgcgcc ggaagaacac gctgaagtcc tcggccgccc ctctcgtctc
    10561 gatcatcacc cgcatcccta caacatgaac cccccacgca ggtcccgcgt ttggtacatg
    10621 ttcggatgat ttgccgctgc cggtccaaac ggagcgaggg ctggtctcac caagtgtcgc
    10681 aggcgggaag cgacatcctc gccggtggct agttctcgta gtacggctcg aacaggacca
    10741 cctcgtcgcc gcggtcaagg atccccagca acgaagcgat catggcctcg gtcgagccgc
    10801 aggtgacgca caccatcgtc tccggatcga cctccatgcc gtactcgcgc cggtacttgg
    10861 ccgcgatggc ctcgaggaac gcgaacgagc cccaggtgat cgggtactgg ttgatgtcgt
    10921 cggcgatcgc agcgcgggcc gcttccttca cctccatcgg cgcggggaag tcggggtagc
    10981 cctgcgacag gttgatcgcg tcgtgcgcga tggccagccg ggtcatctcc cggatcaccg
    11041 actcggtgaa cgaagaggcc ttcgctgagg gacagagttt gggcatcggg ccggatgata
    11101 ccggctccga tatcgttgcc gaagcggcct cacgagacgc ttcccgccag gacgaacggg
    11161 gccatcgcgt acagccggtg ccagccgtgg atcccgagag tcatctcttg gcgactccgc
    11221 tgcccccgct atacgcaagc gcggggaagg gcggatggcc atcgctcgga ttgtcccacc
    11281 gctcttcagc cacaccgcct catggagcag atcgggatca acccaaacct gccgcgtccc
    11341 acgatggaac cccgtatcac gggcacccgc aggtagcggg gcgcggtccg acaacgacgt
    11401 ggcgaggtga tagtggacgg gatcgccgtt ggccacgtac gaaaggatct gttcgtccgc
    11461 ccccgggagc tcaacgacct gccccaccgg ccgtgtcagc cgcagctcca ggacggaggg
    11521 gtcgccgcag gctcggctga gggcgaggac gaaggcctga ccccgggacc gctccggaga
    11581 gcaacggatg ccggggcgcc gatggcccgt gccaggatca cgcacccgag gatgaacgag
    11641 gcgtacagga gagcgactgg acccccgacc gggagccgta gcggatcgag gaacacctgc
    11701 cagctgaacg ggctcgagag ggaccacgcg tgtacgcctc aacgtgcccg ccggaaccgc
    11761 gcgccacccg cttgcggggg cggcatcagg tcgttcctcc gcgaggacac gcggaccgcc
    11821 atctcgccgg acggcgtcga gcggagcgcg atcccggtca gccggccagc acgcgcgaga
    11881 gctcttccag actctccagg ttgtgtccgg gcaggaacac gtccaccgag ggaagcgccg
    11941 ccgccatccc ccgcgccagc ggctcgtagc gcgggctgcc tttgagcggg ttcacccaga
    12001 ccacgcggtg cgccaggcgg tggagccggg ccatctgatc gcggagcaga tcggcgtccc
    12061 ctcgctccag cccgtccgag cagatcaccg ccaccgaccc ccgcaaagcg gcccgctggc
    12121 tccacccgtc caagagctcc ttgagcgaga tcccgatcct ggtgcctccc tcccaatccg
    12181 ccaccatccg accgatctcg tgcagagccc ggtccgggtc cttggtccgc agggtgcggg
    12241 tcacgcgggt gagacgcgtg ccgaagcaga acacctccac ggttctcccg acggccatgg
    12301 cggcgaaggc gaactgcacc agggcccggg cgtagggggc catcgagccc gagatgtcca
    12361 ggatcagcac caagggccga acccgttgcc cccgccgtct ccacgctcgt tggaacggct
    12421 caccctgggt ccggagcgac cggcgaaggg tgcgcttgac gtcgaaccga ggcccggtgg
    12481 ccgcgggatg cagacgccgc gtccggcgca ccggcacgct cacggcgatc cgccggatca
    12541 ggcgggccac acgctcgcgc tcctcatcgg tcaggtccgc gaacgacttc tcccgcagca
    12601 cctcgaccgc gctcgccacc aggcgcaccg cggcctcgcc ctccggctcc tccccctccg
    12661 ccgccgatcg ccactcggcg gcaagggccg gtccctctac cgaaggatcc tccgccccct
    12721 gctgcacctg ctcctgggcg gccgccgccg catcggcacc agggaggtcg agctcgatcc
    12781 gcaccccgtc gcggaagtac gactcgaagg cgcgatcgta ggcctcgacg tcttcggggc
    12841 ggccaaccaa actggctcgc cccgcccagt agaggtcgtt gcgcgagggt ggggacagcg
    12901 cggcgaccgc tcggcagaac gtgacgatcc ggccggtgcc gaccgcgagc cctcggtgcc
    12961 ggagctcgcg tccgaacccg gtcagccggc cgagggggtc gatctcagct gtcgccatcg
    13021 ccgatcacct cggcgatcct ggagcgggcg agctcctgat cctcgtggtc cttcagcacc
    13081 gtgcccaggg tcgcttccac cgtcggctcg tccagacggt cgacccccag gaacgacacg
    13141 gcgttcgccc agtcgatcgt ctcggccaca ccggggcgct tggcgatgtc gaacgagcgc
    13201 aaccgcatca cggccgaggc tacgtcccgc gccagcgcct cggagatctc cggcgctcga
    13261 gcccggacga tctcgatctc gcggtccagc gccgggtgat cgatccagtg gtacaggcag
    13321 cggcgcttca gcgcgtcgtg cagctcgcgg gtccggttcg aggtcaccac caccatcggg
    13381 ggctcggtgg ccccgatccg cccgatctcg gggatcgtca ccgcgaactc ggagaggatc
    13441 tccagcagga aggcctcgaa ctcgtcgtcg gcccggtcga gctcgtccac cagcaggacc
    13501 gccctcgaac cggcccggat cgccgcgagc aggggtcgct ccaccaggaa ctggggcccg
    13561 aacaggtccg cgacgtgggc ctcgacggtc acgtcctgca gggcgcggac cgcgatcagc
    13621 tggcgggagt agttccactc gtacagcgcc tgggacgcgt cgatgccttc gtagcactgc
    13681 agcctgatca gctcccgatc gagcaggtcg gccaggacct ttgccacctc ggtcttgccc
    13741 actccggcct cgccctcgag cagcaggggc ttgtccaggg acgccgccag gtagatcgcg
    13801 gtggccaggc cccggtcggc caggtaccgg cgtccgcgca gcgccgaccg cagctcctcc
    13861 accgatgcgt acggcaggct ccccatcggt cccagcgtac cggctgggca cgcggagcgt
    13921 ggtggcgggg cgcatgcggc acctccgcct gcggtgccgc ggctacgtgg tggcctcggg
    13981 ctgtccggaa gggccgggca tcaccgccgg gggcagcggg atccggatcc cacgctcgga
    14041 caaccgccgc gtgatgcgct cgcgcagggc gcgctcggcg tcccaccgct tggaggggcg
    14101 ggtctcggcc accactcgaa ccaccagggc
//
LOCUS       DASQQQ010000185         7595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_152541, whole genome shotgun sequence.
ACCESSION   DASQQQ010000185 DASQQQ010000000
VERSION     DASQQQ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152541"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VE646_07245"
     CDS             complement(<1..911)
                     /locus_tag="VE646_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZD79820.1"
                     /translation="MSVAVGGVRRDVREGPGDHGGGRGGPARGGRAQIGRGLPAVAFT
                     GLPGAPVHDARDRIRPATESSGCDGPSAGSWSTCRRGTSGRTVRGLDLPVTMGVLAAS
                     AQVRSDHLERYAFYGELALDGSLGPTPGVLPVAVAAWRAGLAGVVVPRANASEAALVE
                     DLHVVGAPDLASVVAFLRDRWDPPLAQPGAPAPPSHPVDLEEVRGQARARRALEVAAA
                     GGHNVLLMGPPGAGKTMLARRLPTVLPTLSRSEALEVTQLHSVAGILPAGGLVAARPF
                     RAPHHSISVSALLGGGSGPVRPGEISMA"
     gene            complement(918..1649)
                     /locus_tag="VE646_07250"
     CDS             complement(918..1649)
                     /locus_tag="VE646_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZD79821.1"
                     /translation="MQESWLGVPGDTGNRLLDALPAGQRVHLVRCMRAVPLRLRQVVH
                     EQGHPIEHVVFPLDGVISLVVAMEDGSAVETATVGREGLVGLPVCLTEDGVATHRAVV
                     QIQGEGLAIDAAAFLAELGDLPAMRRIVAGYANALLAQVSQSVACNGSHTLRQRCARW
                     LLQTGDRVGSECFYLTQEFFAQMLGVRRASVTVIAGSLRDAGLIRYRRGRVEIVDRPG
                     LEALSCECYRVVRDHYQRLIPGPER"
     gene            1752..2069
                     /locus_tag="VE646_07255"
     CDS             1752..2069
                     /locus_tag="VE646_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZD79822.1"
                     /translation="MVPFRVDRGAIPGSFRLVGELDVAVAELLDTLVQTPPQGDLRID
                     LSGLAFMDSSGLRALIKMAKGLRDRGAKLVLEGSSGEVARLLQLVRADTFPGVVVIPA
                     LDS"
     gene            complement(2190..2525)
                     /locus_tag="VE646_07260"
                     /pseudo
     CDS             complement(2190..2525)
                     /locus_tag="VE646_07260"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
     gene            complement(2554..2988)
                     /locus_tag="VE646_07265"
     CDS             complement(2554..2988)
                     /locus_tag="VE646_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HZD79823.1"
                     /translation="MGVSAVVLLASACGGGSSGEAAAADCAEGAQVVRVQAMDTLRFD
                     PDSVSVKAGEPVCFVVTNEGDIKHEFVVGGKEMQDEHEQEMAGGASMGEMDMGDLPEL
                     ELEPGETKSVTTTFDEPGQMLFACHEPGHYAGGMVGTLTITA"
     gene            complement(3226..3816)
                     /locus_tag="VE646_07270"
     CDS             complement(3226..3816)
                     /locus_tag="VE646_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZD79824.1"
                     /translation="MLDALLDPPVRWVHLVSAVLWPASALIGLWRTRSLRRRRSRRIT
                     YALVVVAFAVGAENLMQALSKAHPAGWAHLTANIVLSGAGVIAVFAMAAYGTEVVVEE
                     RILHALSRGPDADEPPEPRDPLTPREWEVLGLLCQGLGSEPIAARLGISPNTADTHIR
                     NLMRKLKVSSRASAVGWAVRSGAFDPSTGRLGSGVG"
     gene            complement(4072..4686)
                     /locus_tag="VE646_07275"
     CDS             complement(4072..4686)
                     /locus_tag="VE646_07275"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HZD79825.1"
                     /translation="MRPIPVLFHLGPLQIHTYGIGLAVTFIFAAWYLGRRFRRAGYPW
                     RWVGDAAFWIVIAAMVGARVVHVVSQWSFYAAHPGQIPAIWNGGLSSFGGLLFALPTG
                     LVLMRRRCPGVSLGRGLDLAAPVLAAGWAMGRLLGPQLMISGGGRPTTAWYGMRYAGS
                     AGPRVPVPIFQSIGAFLVLAAVLLVERRMHQRPDGLLIAVAAAL"
     gene            complement(4708..5391)
                     /locus_tag="VE646_07280"
     CDS             complement(4708..5391)
                     /locus_tag="VE646_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79826.1"
                     /translation="MWVLLLSVPVVLITLSFAVTPGTPTPVDIGGSTTMVPMVDLHGA
                     IMVPITVAFLAGLAGLFVVLGSAEGDRRLVLSGFRSGEVLAARIGVIVLAAVLSSAVS
                     LVVTAADLRPQAWVPFAGASLLVGLTYAMIGVLAGPLVGRLGGLYLMFLIPMLDVGIA
                     QNNMFGAALPSWGRFLPAHGAVQVLLNGAFSSSFTETGALLQTIGWLVGITAVAAMVF
                     RRVAEPERA"
     gene            complement(5355..6191)
                     /locus_tag="VE646_07285"
     CDS             complement(5355..6191)
                     /locus_tag="VE646_07285"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD79827.1"
                     /translation="MTALLTRRYLVEYARRPVNVILLVVVPVVFVALSAGVVADFAKL
                     LGGTGDTPSLEAATAGWAGAFLAGVGGFFHVAGSRRADRRLALAGTRPVKIVVARLAS
                     GLLLVLLAAGGALLALAARTGIVDVPRTVGATVMFAVVYLGIGAAVGALVHDEVNGSL
                     IILFVWILDVFLGPAMAGGTSALTRVLPTHFVTLVLMDAASGHAGALGDLGAALLWTF
                     GGLAASAAIFVRTTRAQEAQIGSEREPAGGAGWPRGSGTGSASTGGTSSCGCCCSRCR
                     WC"
     gene            complement(6188..6919)
                     /locus_tag="VE646_07290"
     CDS             complement(6188..6919)
                     /locus_tag="VE646_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD79828.1"
                     /translation="MLFWVRAHMSVSEIALAVPRPECGEAREGAADGGHVLRANGVTK
                     TFARGMWPRRHVLHVLRGASLELGRGEIVGLVGENGSGKSTLLKILSGALPREGGTIE
                     RRGRIGYCPQDPILYERLTCDEHFELFGRAYGMDRPRVEEARRAIYDILGFGGWAAAR
                     VAELSGGTRSKLNLGLALLPDPGILLLDEPYAGFDWETYQRFWYLAAERRDAGRAVLI
                     ISHFVTDAERFDRIYEMREGRAVAR"
     gene            complement(7055..>7595)
                     /locus_tag="VE646_07295"
     CDS             complement(7055..>7595)
                     /locus_tag="VE646_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HZD79829.1"
                     /translation="IGTGTDVAIESSDITLISGDLDGVVTAITLSRATMRNIRQNLFW
                     AFGYNTVGIPIAAGVLYPAIGLLLNPMIAAGAMALSSLSVVANANRLRRFRPAPIGSL
                     EAPTPDVKPTVEVREHEGQEDDSEEKEMETMTVHDPVCGMEIDPSNAAASEEYEGQTY
                     YFCSQSCHEKFLADPGQYR"
BASE COUNT         1222 a         2897 c         2521 g          955 t
ORIGIN      
        1 gccatggaga tctctcccgg ccggaccggt ccgctgcccc cgcccagcag ggccgaaacc
       61 gagatggagt ggtgcggcgc gcggaacggc cgtgccgcca ccagccctcc ggcgggcagg
      121 atccccgcga ccgagtgcag ctgcgtcacc tccagcgcct cggaccggga cagggtgggc
      181 agcaccgtgg ggagccgcct ggccagcatg gtcttcccgg cccccggagg ccccatgagc
      241 agcacgttgt gaccgcccgc cgccgccacc tccagggccc gccgggcccg cgcctgtccc
      301 cggacctcct ccaggtccac cgggtgcgac ggcggcgccg gcgctcccgg ctgcgccagg
      361 ggtgggtccc agcggtcccg gaggaaggcc accacgctgg ccaggtcggg ggctcccacc
      421 acgtgcagat cctccaccag cgccgcctcc gatgcgttgg cccgggggac gaccaccccc
      481 gccaggccgg cgcgccaggc ggcgacggcc accggcagga ccccgggggt gggcccgagc
      541 gatccgtcca gggccagctc cccgtagaag gcgtagcgct ccaggtggtc cgagcgcacc
      601 tgcgcggagg cggccaggac ccccatggtc accgggagat ccagcccccg gaccgtcctt
      661 cctgaggttc cccggcgaca ggttgaccac gatccggcgg aggggccatc gcagccggag
      721 ctctcggtgg ccggccggat ccggtcgcgg gcatcgtgca ccggggcccc gggcagcccg
      781 gtgaaggcca ccgcgggcag gccccggccg atctgggctc gaccgccacg ggcaggcccc
      841 ccacgcccac caccgtgatc cccaggacct tcccgtacat cccggcggac cccaccgacg
      901 gccaccgaca cccggtgtca gcgctcgggc cccgggatta gccgctggta gtggtcgcgg
      961 accacccggt agcactcgca ggagagcgcc tccaggcccg ggcggtccac gatctccacc
     1021 cggcctcgcc ggtacctgat cagacccgca tcgcggagcg agcccgcgat cacggtgacc
     1081 gaggcccggc gcacccccag catctgggcg aagaactcct gcgtcaggta gaagcactcg
     1141 gagcccaccc ggtcgccggt ctggagcagc caccgagcgc accgctgccg gagcgtgtgc
     1201 gagccgttgc acgcgaccga ctgcgagacc tgagcgagca atgcgttggc gtagccggca
     1261 acgatccgtc gcatggcagg gagatcgccc agctcggcca ggaaggccgc ggcgtcgatg
     1321 gcgagcccct ctccctggat ctgcaccacg gcccgatggg tggccacgcc gtcctcggtc
     1381 agacacacgg gcagtccgac caagccctct cgacccaccg tggccgtttc caccgccgag
     1441 ccgtcctcca tggccaccac cagggagatc accccatcga gggggaagac gacgtgttcg
     1501 atcggatgcc cctgctcgtg caccacctgg cggagacgca gcgggacggc acgcatgcac
     1561 cgcacgaggt ggacccgctg ccccgccggc agggcatcga gcaagcggtt cccggtgtcg
     1621 cccggcaccc caagccagct ctcctgcatg ctccgaagca tgccacgcgc cgggcacctt
     1681 gcgtacgccc ccgtaccgac aggcccgtcc ggcgacggta gcgtgcccga agatcaggcc
     1741 gagaggagac catggtgcca ttccgggtgg atcggggggc catccctgga agcttccggc
     1801 tcgtgggcga gctggacgtg gccgttgccg agctcctgga cacgctggtc cagaccccgc
     1861 cgcagggcga tctgcggatc gacctgtcag ggctggcgtt catggacagc tcggggctcc
     1921 gggccctgat caagatggcc aagggcctcc gggaccgggg cgccaagctg gtgctggagg
     1981 gctcctccgg cgaggtggcc cgcctcctgc agctggtgcg ggccgacacg ttccccgggg
     2041 tggtggtgat cccggccctc gacagctgac ggctcagaac gcgtcttcga acagctcgac
     2101 ccgcggcgcg cgggcgagga cagcacccga acgctggcca cgtcgaaccg cacgtccccg
     2161 ggccgtgcgc ccgtggtcgc gatgaacagc tcggcgagac gacgcaggcg ttcccgtttg
     2221 gccgcgccca ccgcctcgaa cccgccaccg aagcgatcgc cgcgccgggt cttgacctcg
     2281 cagaagacca gcgtgccgcc gcgctccacc accagatcga gctctccggc ccggcaccgc
     2341 cagttccgcg ccacgggtgc ggaacccccg agcgcggtag gcggccaggg cggcgtcctc
     2401 ccccccgcgg cccgtggcca gccgggggtc gggaccggtc ttttcgccgg aaggctccac
     2461 ggcacgtgat gctgggcggc tgcccacacg ccaagacggt gcgttcggcg cccctggggc
     2521 gccatgcggg ccgacgatgc ctcgtccgag cgcctacgcg gtgatcgtca gggtcccgac
     2581 catcccaccc gcatagtgac cgggctcgtg acaggcgaac agcatctggc ccggttcatc
     2641 gaacgtcgtg gtgacggact tggtctcccc cggctcgagc tcgagctcgg gcagatcgcc
     2701 catgtccatc tcacccatgc tggcgccgcc agccatctcc tgctcgtgct cgtcctgcat
     2761 ctccttgccg ccaaccacga actcgtgctt gatatcgcct tcgttcgtga ccacgaagca
     2821 cacgggctct ccggccttga ccgacacgga gtcggggtcg aaccgaaggg tgtccatcgc
     2881 ctggacccgc accacctgtg caccctcggc gcagtccgcc gctgccgcct cgccggagga
     2941 gccgccccca cacgcggatg cgagcaacac cacagcggat acgcccatca agccgatcga
     3001 acgggatcgc acatccccca cctctttcca gcgtcggtcc cgagccgccg tacggtgcga
     3061 cgtcctgaca tcgcgcggct cctcccaagg tggggtcgac cgaaccgctc tggcaatgcc
     3121 atgtcgccgg tatccgacta ccggatgggt ggtatcccga gccggtaccg tcctgcggcc
     3181 gccccacgag ccccggtcgg gcccggcagg ccggccgttc tcctctcacc ccacgccgga
     3241 gccgagcctg ccggtggacg gatcgaaggc gcccgatctg accgcccacc cgacggcgct
     3301 cgccctgctg gacaccttga gcttcctcat caggttgcgg atgtgcgtgt cggcggtgtt
     3361 cggggagatc cccagacgag ccgcgatggg ctcggacccc agcccctggc acagcagtcc
     3421 gagcacctcc cactcgcgtg gcgtcagcgg gtcacggggt tcgggaggct catcggcgtc
     3481 gggaccacgt gacagggcgt ggaggatgcg ttcctcgacc accacctcgg tcccgtacgc
     3541 ggccatggcg aacacggcga tcaccccagc accgctcagc acgatgttgg ccgtcaggtg
     3601 cgcccatcct gccggatgcg ccttggacag agcctgcatc aggttctcgg cgccgaccgc
     3661 gaaggccacc accacgagcg cgtacgtgat ccgacgggac cggcgacgtc gcagggacct
     3721 cgtcctccac agaccgatca gagcactggc gggccacagg acggcgctca ccaggtgcac
     3781 ccatcggacc ggcgggtcga ggagggcatc cagcacgtca ccgagtgtag gcggctcgcc
     3841 cgggtacatg ccagggtcgg ccccggatca cccgtcggtg ccccgaggcg tgcggtcggg
     3901 aacagcccgg acgcctggtc accccgctgg gacgtcgctg cgggggtgca gcagcagcga
     3961 gcccagcgcc atccagccga tcaggctcag gagcagtccg gcgacctcca ccgcgtcgat
     4021 ccggcccggg gttcccagcc acaggaactg atccaggaaa cggcacgccc ctcacagcgc
     4081 cgccgccaca gcgatcagca gcccgtccgg tcgctggtgc atccgccgct cgaccagcaa
     4141 gaccgcagcg agcaccagga aggcgccgat cgactggaag atcggtaccg ggacccgggg
     4201 ccccgccgag cccgcgtacc gcatcccgta ccaggcggtg gtgggacgac cgcccccaga
     4261 gatcatcagc tggggaccga gcagccgtcc catcgcccag cccgcggcga gcaccggcgc
     4321 ggccaggtcc agaccgcgcc ccagggacac tccggggcag cgccgtcgca tcaacaccag
     4381 cccggtgggg agcgcgaaca gcaggccccc gaacgaggac aagcccccgt tccagatggc
     4441 agggatctga ccggggtgag cggcgtagaa cgaccactgg gagaccacgt gcaccacccg
     4501 ggcgcccacc atcgccgcga tcacgatcca gaacgcggca tcgccgaccc atcgccaggg
     4561 gtacccggcc cggcggaacc gccggcccag gtaccaggcg gcgaagatga acgtgacggc
     4621 caacccgatc ccgtacgtgt ggatctgcag cgggccgagg tggaacagga ccgggatggg
     4681 tctcatcgga tgcgtccacc catcacgtca cgcgcgctcc ggctccgcga cccgacggaa
     4741 gaccatcgcc gccacggcgg tgatcccgac cagccagccg atcgtctgga gcagcgctcc
     4801 ggtctcggtg aaggagctcg agaacgcgcc gttcagcagg acctggaccg ccccgtgggc
     4861 ggggaggaac ctgccccacg agggcagggc tgccccgaac atgttgttct gggcgatgcc
     4921 cacgtcgagc atggggatca ggaacatcag gtacagtccg cccagccgcc cgaccagcgg
     4981 gcccgccagg acgccgatca tcgcgtaggt gaggcccacc agcaggctcg ctcccgcgaa
     5041 tggcacccag gcctggggtc gaaggtccgc cgccgtgacc accagcgaca ccgcggaact
     5101 cagcaccgcc gcgagcacga tcacgccgat ccgcgcggcc aggacctcgc ccgagcggaa
     5161 cccggatagg acgagccgcc gatcgccctc ggccgatccc agcaccacga acagccccgc
     5221 caggccggcc aggaacgcca cggtgatcgg caccatgatc gctccatgca ggtccaccat
     5281 cgggaccatg gtcgtcgacc cgccgatgtc cacgggggtc ggggtgccgg gggtcacggc
     5341 gaagctgagc gtgatcagca ccaccggcac cgagagcagc agcacccaca tgacgaggtt
     5401 ccgccggtac tcgcggaacc cgtaccggag ccccgcggcc aaccggctcc accggccggc
     5461 tcccgttccg acccgatctg ggcctcctgg gcgcgggtgg tacggacgaa gatcgcggcc
     5521 gaggcggcca gccctccgaa cgtccacagc agcgcggctc cgaggtcgcc gagcgcaccg
     5581 gcgtggcccg aggccgcatc catcagcacc agggtcacga agtgggtggg cagcacgcgg
     5641 gtgagggccg aggtcccccc cgccatggcc ggtcccagga acacgtccag gatccacacg
     5701 aacaggatga tgagcgagcc gttgacctcg tcgtggacca gggcccccac cgcggctccg
     5761 atgcccaggt agaccacggc gaacatcacg gtggccccca ccgtgcgggg aacgtcgacg
     5821 atcccggtgc gggccgccag cgccagcaga gcgcccccgg cggcgaggag caccagcagg
     5881 agcccggagg cgagtcgtgc gaccacgatc ttgaccggcc gcgtccccgc gagcgccaag
     5941 cgacgatccg cacggcggga gcccgcgacg tggaagaacc cgcccacacc ggccaggaac
     6001 gcccccgccc aaccggccgt ggctgcctcc agggacgggg tgtcgccggt cccaccgagc
     6061 agcttggcga agtcggccac cacccccgcg gacagggcca cgaagaccac cggcaccacc
     6121 accagcagga tcacgttgac cggccggcgc gcgtactcga ccaggtagcg gcgggtgagc
     6181 agggcggtca tctggccacc gccctgcctt cccgcatctc gtagatccgg tcgaagcgct
     6241 cggcatcggt cacgaaatgg ctgatgatca gcaccgcccg cccggcgtcc cggcgctccg
     6301 ccgcaaggta ccagaaccgc tggtaggtct cccagtcgaa cccggcgtaa ggctcgtcca
     6361 gcagcaggat cccggggtcc gggagcagcg ccagccccag gttcagcttc gagcgggtcc
     6421 ccccggacag ctcggccacc cgggcggccg cccacccccc gaacccgagg atgtcgtaga
     6481 tggcccggcg ggcctcctcg acccgaggcc ggtccatgcc gtaggctcgg ccgaacagct
     6541 cgaagtgctc gtcgcaggtc aggcgctcgt acaggatcgg atcctgcggg cagtacccga
     6601 tccggccacg acgctcgatg gtccccccct ccctgggcag ggcacccgag aggatcttca
     6661 gcagggtgct cttgccggag ccgttctcac cgaccaggcc gacgatctcg ccgcggccca
     6721 gctccagcga ggctccccgc aggacgtgga ggacgtgtcg gcgaggccac atgccccggg
     6781 cgaaggtctt ggtcaccccg ttggcacgga gcacgtgccc gccgtccgcc gccccctcgc
     6841 gcgcctcacc gcactcgggt cgcggcaccg ccagcgcgat ctcactcacg ctcatgtgcg
     6901 ctcggaccca gaacagcacc ccgtcccctc gtcctcatgt tgcccatcgc atgggtggtc
     6961 cggtccatga tcctcgcctg gttcacaccg acatccatcc ctgccgcaac ccatcactcc
     7021 tccttcgatg tcgggccgac gccatgagcg gcggctaccg gtactggccc ggatcggcca
     7081 ggaacttctc gtggcagctc tgggagcaga agtagtaggt ctggccctcg tactcctcgc
     7141 tcgccgccgc gttcgagggg tcgatctcca tcccgcacac cggatcgtgc accgtcatgg
     7201 tctccatctc cttctcctca ctgtcgtcct cctgtccctc gtgctcgcgg acctccacgg
     7261 tgggcttcac atctggggtc ggcgcctcga gcgatccgat gggtgccggc cggaaccgcc
     7321 gcagacggtt ggcgttggcc accacggaga gcgacgacag ggccatggct ccggccgcga
     7381 tcatcgggtt caggagcagg ccgatcgccg ggtacagcac ccctgccgcg atggggatgc
     7441 ccaccgtgtt gtagccgaag gcccagaaca ggttctggcg gatgttgcgc atcgtggccc
     7501 gcgacagggt gattgccgtg accacgccat ccaggtcgcc agagatcaac gtgatgtccg
     7561 acgactcgat ggccacgtcg gtccccgtgc cgatg
//
LOCUS       DASQQQ010000186        10818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_228799, whole genome shotgun sequence.
ACCESSION   DASQQQ010000186 DASQQQ010000000
VERSION     DASQQQ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10818
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228799"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..289)
                     /locus_tag="VE646_07300"
     tRNA            complement(217..289)
                     /locus_tag="VE646_07300"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(253..255),aa:Glu,seq:ctc)
     gene            356..913
                     /locus_tag="VE646_07305"
     CDS             356..913
                     /locus_tag="VE646_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HZD79830.1"
                     /translation="MSEPADPRTIFTIGHGTRPIGDFLELLRGAGIQRLVDVRTAPGS
                     RRNPQFGKEELAAALADASIAYDWEKDLGGLRRTGPDSRHTAIRNASFRAFADHMETD
                     TFRGALDRLAETSRDTITAIMCAESLWWRCHRRMIADALTVRGWRVIHLMDRGKREPH
                     RLHPNARVVGEEVIYDVEDQPRRSG"
     gene            969..2480
                     /locus_tag="VE646_07310"
     CDS             969..2480
                     /locus_tag="VE646_07310"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZD79831.1"
                     /translation="MAVSTPSGRSRALRRITIPVVGLTLFALIAACTASTASEGATGA
                     TGATGTPVAEAAASPPPKLAGFRNIQHVIFIVQENRSFDHYFGTFPGADGIPMKNGKP
                     TVCVPDPVLNECVKPFHESALVNQGGPHDLTHSRIDVDGGKMDGFVRSVTLYSPNKCA
                     DTRSVADCGDTLGPQGQPDVMGWHDAREIPNYWTYARRFVLQDRMFAPTDSWTLPAHL
                     YLVSGWAAHCSSPWKPMTCSSDLQQQGMLDKQRHGQHPAIYAWTDITWLLHRAGVSWA
                     YYAGQNLCGPEVPAKECQSGGATPAQNPLPSFTDVHQTKQMAKVQTHDDFFNAVADGT
                     LPQVSWVVPGRGGISEHPGTHAPLTKGQAWVTRVVNAVAESPLWYRTAIFLTWDDWGG
                     FYDHVRPRRVDANGYGIRVPGLMISAWARRGKIDHQTLSFDAYLKFVEDLFLNGRRLN
                     PRWDGRPDSRPTVRENVKILGDLRKEFDFSQDPLKPPILDPRPSPGPASIPGS"
     gene            2543..3991
                     /locus_tag="VE646_07315"
     CDS             2543..3991
                     /locus_tag="VE646_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018761698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZD79832.1"
                     /translation="MALIATGVVLKVDSGRRASASSRASSSPPTAASTGSAPTQLQLA
                     KQHLKHVIFIVQENRSFDHYFGTFPGADGIPMKDGTPTVCVPDPLLNRCDRPYHSSNL
                     VNFGGPHTQRHSIADVDGGKMDGFVGTAVASHIRCAQAPGRFQPKCRSYLGPRLQPDV
                     LGWHDAREIPNYWGYAEHFVLQDHMFAPTDSWTLPSHLFLISAWSASCTNPKDPMSCH
                     SDLELKEAAKVQHGGQEPLYGWTDITYLLNREGVSWRYYVGEGTCLVDPCSKPRAGSH
                     YTVYQQDPLPGFVTVRQDHQLRNVAGHPAYFRAAADGALPSVSWVMPYNGVSEHPSNG
                     KPITDGQAWVTRVVNAAMRGPDWDSTAIFLTWDDWGGFYDHVRPPRVDRNGYGLRVPG
                     ILISPWAKAGTIDHQTLSFDAYLKLIEDLYLGRQRLNPRTDGRPDPRPTVRENAKILG
                     DLLKEFDFSQTPLDPLVLDPRPSPGPASVPGG"
     gene            complement(4038..5447)
                     /gene="hydA"
                     /locus_tag="VE646_07320"
     CDS             complement(4038..5447)
                     /gene="hydA"
                     /locus_tag="VE646_07320"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HZD79833.1"
                     /translation="MLVRGGTIVEPSGTRRADVLIEGERIAAVGPDLDPAGHGVVDAS
                     GALVVPGAIDVHTHLDLPVGTAVSADDFASGTTAAACGGTTCVIDFAGAGREPPDEAL
                     RTWHAKAEGRAVVDYGFHLTVTSVPEDPADAEELFRWFAAQGVASVKLYLAYPERLMV
                     DPPTLERALAAGKRAGVLVCVHAEDGLEADRRTRRLLASGKTGPSGLIEARPPEVEAA
                     AIRLAAQLAERAAASLYVVHLSSEAGLREIRTARRRRVLVLAETCPQYLYLTDDALRG
                     TGLTGRSQEAADFVCVPPLRTELDREALWGALADGELDAVSTDHCPFTRDTRRRGTQA
                     GGARWKDFTQIPGGLPGVETRLSLVYQGVIDGRFGPERWVDLVAAAPARLFGLARRKG
                     RLAPGLDADLVVFDPSATKHLAAGDLHSRTDHSPYADLTVTGWAAITVSRGRVVARGG
                     EPADVEPGWGRFVPRDRVG"
     gene            5500..6996
                     /locus_tag="VE646_07325"
     CDS             5500..6996
                     /locus_tag="VE646_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008387954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZD79834.1"
                     /translation="MEPVQLQRDLVDPAVLGRAVERFRRDGIVLPTFEQLAEPEKIPE
                     PIRASLSSVDPDAPDPRNLFRVHWYNAADRRGQVPVPGHLVLPPELTGVPARIVLALG
                     DRFPMIGAHKVLAAYGVLAPRLVTGSFDPTSQRAVWPSTGNYCRGGVAISRIMDCRGV
                     AVLPEGMSRERFEWLARWVADPSDVIRTPGTESNVREIYEECARLERDPDNVILNQFS
                     EFGNHLVHYLCTGAALEHVFEAMREEQGDLHLRAFVSASGSAGTLGAGDYLRERYGST
                     TVAVEALECPTLLYNGFGEHNIQGIGDKHVPFIHNVAGTDVVTAVSDRDTDRLNVLFN
                     TEVGRRFLRRRGIAPDVIAALDSLGLSSICNALAAIKVARYFGMGPDDVIVTVATDGA
                     ALYRTEVDKVLARDFGGSFNEADAAETFGRSLEAVTTDHLLELTERDRRRIFNLGYFT
                     WVEQRGVSVEDFTLRRSPSFWRGLRGLLPIWDDMIDELNGRTGLGVGG"
     gene            6993..8342
                     /locus_tag="VE646_07330"
     CDS             6993..8342
                     /locus_tag="VE646_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZD79835.1"
                     /translation="MTAEGTAVVCSGCGYRAPADEPYPFQCPGASSGDDIDHLMVRVL
                     DPEHVSFPWDGDPNPFTRFRRLFHAYHLGLRHGMLDDEYVDLVRDLDRRVAKVDGQGF
                     STTPFSRADELSERAGFAPEGGIWVKDETGNVSGSHKARHLMGVMIHLLTMEWLSLAP
                     SDRRPDLAISSCGNAALAAAVVANAAGWPLQVFVPAWADPAVLDRLVSLGARVTTCER
                     RPGVSGDPAYHALRRAMDEGALPFSTQGPDNGLTIEGGETLAYELVTDLAAEDRAMDR
                     LVIQVGGGALASSCIRGFEDAALLEAPVRLPRIHAVQTEGAHPLERAFRLVRERAGAE
                     PGPSAVVDAMAYARTHRSGFMWPWESEPHSIATGIVDDETYDWAAVVGGMLATDGGPV
                     LASEDRLREANDLARRTTGIDVDPTGSAGLAGVLSLLEEDAIGPAERIAVLFTGVRR"
     gene            8395..9348
                     /gene="pyrB"
                     /locus_tag="VE646_07335"
     CDS             8395..9348
                     /gene="pyrB"
                     /locus_tag="VE646_07335"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HZD79836.1"
                     /translation="MRSFLGRDILSLKDFEREEFFHVFEICDRLAPIAKERRNADLLK
                     DKTLLTAFYQPSTRTRMATEAAMHRLGGHVLGFSDAKMTRAGDFYQESIKDTVHMLEF
                     YADVIAMRHFQQGAPEEAARWASVPVINCGDGWGEHPTQVLTDLYTTWKELGRLDGLK
                     FLLVGDMRMRTMHSILYAFSRFDVESLVVSPPDMSLLPEFKAELEGRSVPFREVESVE
                     KGIADADVIYMEPVVQADYTKGRDERGAEVPTTPDAYRVTRELLRDKGKAEAIVLHSL
                     PRMDELSVEVDATRHARYWVEAFNGVVMRMALLSLVLGAVE"
     gene            9380..>10818
                     /locus_tag="VE646_07340"
     CDS             9380..>10818
                     /locus_tag="VE646_07340"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZD79837.1"
                     /translation="MRFRLNGRPVRVEIRPGMSLLELLREGCGVTSVKDGCAPEGSCG
                     ACTVLVDGRAVVSCAQAAGRAEGRDVLTQDGLSEEQRGTWADAFVASGASQCGFCSPG
                     IVLKAESLLAKNPRPSREEVARALAGNLCRCTGYVKIVDAVELVAGARRGEPLPAVDL
                     SGRVGSRTARYQGRELALGDKPYVNDLSAPGMLHGAIRFSNHPRARVLRIDVSKARAH
                     PGVMAVATAADVPGERSQGLITRDWRQLVAEGEVTAYVGDVLAVVAARSRHAAREAAQ
                     LVEVEYEVLEPVTDPHRALRPEAPSVHEDRDNLLSTSVVRRGDAEAVLAGSTHVLSET
                     FQTQVIEHAFLEPESALAVPEDGGMHVYSQGQGVWEDRRQIASFLGVAEGEVRVTQVA
                     NGGAFGAKEDLNVQCHAALLAHLTSRPVLLTLSRRESLGFHPKRHPMTMQYTIGADEE
                     GRLTAVRARIVGDTGAYASVGDKVLERAAG"
BASE COUNT         1563 a         3778 c         3891 g         1586 t
ORIGIN      
        1 cgccacccca gcccccaagc gctggatcgc ggaatgctcg acacgcggac cgttacggtt
       61 cgagggctct atctcctgtc ccgacatttc aaggaccgcc gtcccacgtt caaacagacc
      121 cgccccctgt ccgggtagaa gcagcgggca gaacgatgcg gagtaggaag gcgtccgggc
      181 agctcctccg aggcgtctcc gcaggtcgga gccctgtagt cccaacggga tttgaacccg
      241 tgtctccacc ttgagagggt ggtgtcctgg gccactagac gatgggaccc tgagccgccc
      301 ggcggcggca agtgtagcaa cgacccggtc gcgccagccg tcttacgctg gagtcatgag
      361 cgagccggcg gatccccgga cgatcttcac gatcgggcat ggcacgaggc cgatcggcga
      421 cttcctcgag ctgctccgag gtgccgggat ccagcggctg gtcgacgtcc gaacggcgcc
      481 cggctcccgc cgcaacccgc agttcggcaa ggaggagctg gcggccgccc tcgcggatgc
      541 ttccatcgcc tacgactggg agaaggacct gggcgggcta cggcggaccg gtcccgactc
      601 gcgccacacc gcgatccgca atgcgtcctt ccgggcgttc gcggatcaca tggagaccga
      661 cacgttccgc ggagccctgg atcggttggc cgagacctcg cgcgacacga tcaccgcgat
      721 catgtgcgcg gagtcgctgt ggtggcgctg ccaccgtcgc atgatcgccg acgccctcac
      781 cgtccggggt tggcgggtga tccacctgat ggaccgaggc aagcgggagc cgcaccggct
      841 gcatcccaac gcccgggtgg tcggtgagga ggtgatctac gacgtggagg accaaccgcg
      901 ccggtcgggg tgacccgtcc ctggctccca ccgcttttct ggatccgctg atatggtcgg
      961 ggggctccat ggctgtatcg accccctcag gacgctcgcg agccctgcgc cggatcacga
     1021 tccccgtcgt cgggctgacg ctcttcgcgc tgattgccgc ctgcacggcc tccacggcca
     1081 gtgagggcgc gaccggcgcc actggtgcca ccggcacgcc cgtcgcggag gcggcggcct
     1141 cgccgccccc gaagctcgcc ggcttccgga acatccagca cgtgatcttc atcgtgcagg
     1201 agaaccggtc cttcgatcac tacttcggca cgttcccggg ggccgacggc atccccatga
     1261 agaacggcaa gcccaccgtg tgcgtgcccg accccgtcct gaacgagtgc gtgaagccgt
     1321 tccacgagtc cgcgttggtg aaccagggtg gaccgcatga cctgacgcac tcgaggatcg
     1381 acgtggacgg cgggaagatg gatggcttcg tccgatccgt gaccctctac tcgccgaaca
     1441 agtgcgccga cacgcgttcc gtggccgact gcggcgacac cctgggtccc cagggccagc
     1501 cggacgtgat gggctggcac gacgctcggg agatccccaa ctactggacg tacgcccgcc
     1561 gcttcgtgct gcaggaccgc atgttcgcgc cgaccgactc gtggaccctg ccggcgcacc
     1621 tgtacctggt ctcggggtgg gccgctcatt gctcgagccc ctggaagccg atgacctgca
     1681 gctcggacct gcagcagcag gggatgctgg acaagcagcg ccacggccag cacccggcga
     1741 tctacgcctg gaccgacatc acctggctcc ttcaccgggc cggggtgagc tgggcctact
     1801 acgcgggcca gaacctctgc ggtccggagg tgccggccaa ggagtgccag tcgggcgggg
     1861 ccacgccggc ccagaacccg ttgccgtcgt tcaccgacgt ccaccagacc aagcagatgg
     1921 ccaaggtgca gacgcacgac gacttcttca acgcggtggc cgacgggacg ctgccacagg
     1981 tctcctgggt ggtgccgggt cggggtggga tcagcgagca ccccggtacg cacgctcccc
     2041 tcaccaaggg ccaggcctgg gtgacgcggg tggtgaacgc ggtggccgag agccccctct
     2101 ggtaccgcac cgcgatcttc ctgacctggg acgactgggg cgggttctac gatcacgtcc
     2161 gcccccggcg ggtcgacgcg aacggctacg ggatccgggt tccgggcctg atgatcagcg
     2221 cctgggccag gcgggggaag atcgaccacc agacgctgtc gttcgacgcc tacctgaagt
     2281 tcgtggagga cctgttcctg aacggtcgac gtctgaaccc tcggtgggac gggcggccgg
     2341 actcgcgccc cacggtgcgg gagaacgtca agatcctggg cgacctgcgc aaggagttcg
     2401 acttctcgca ggatccgctc aagcccccga tcctcgatcc caggccgagc cccgggccgg
     2461 cctcgatccc gggctcgtga ggcccgtgcg acgtccgccg ttgcggcgcg gggtgctcgg
     2521 cgccggcgtc gtagccgtcg ccttggctct gatcgccacc ggggtcgtgt tgaaggtgga
     2581 ctcgggccga cgggcctccg cctcgtcccg ggcctcgtcc tccccgccca ccgccgcctc
     2641 caccggctcg gctcccaccc agctgcagct ggcgaagcag cacctgaagc acgtgatctt
     2701 catcgtccag gagaaccggt ccttcgatca ctacttcggc acgttccccg gggcggacgg
     2761 catccccatg aaggacggca cgcccaccgt gtgcgtgccc gacccgctgc tgaaccggtg
     2821 cgaccggccc taccattcgt cgaacctggt caacttcggc gggccccaca cccagcggca
     2881 ctcgatcgcc gacgtcgacg ggggcaagat ggacgggttc gtggggaccg cggtggccag
     2941 ccatatccgg tgcgcgcagg cccccgggcg gttccagccc aagtgccgga gctacctggg
     3001 cccccggctg cagcccgacg tcctgggctg gcacgatgcc cgggagatcc ccaactactg
     3061 gggctacgcc gagcacttcg tgctccaaga tcacatgttc gctccgaccg actcgtggac
     3121 cctgccctcg cacctgttcc tgatctcggc ctggtcggcg tcgtgcacga acccgaagga
     3181 tccgatgagc tgccactcgg acctggagct gaaggaggcc gcgaaggtcc agcacggcgg
     3241 ccaggagccg ctgtacggct ggaccgacat cacttacctg ctgaaccgtg agggtgtgag
     3301 ctggcgctac tacgtggggg aaggcacctg cctggtcgat ccgtgctcca agccccgcgc
     3361 ggggagccac tacaccgtgt accagcagga ccccttgcca ggcttcgtca cggtccgtca
     3421 ggatcaccag ctgcgcaacg tggccgggca cccagcctac ttccgggccg cggccgacgg
     3481 cgccctgccc agcgtctcct gggtgatgcc ctacaacggc gtcagcgaac atccctcgaa
     3541 cggcaagccg atcaccgacg gccaggcctg ggtcacccgc gtggtcaacg cggccatgcg
     3601 gggtcccgac tgggacagca cggccatctt cctgacctgg gacgactggg gcggcttcta
     3661 cgaccacgtg cggccgccac gcgtggaccg caacggctac ggccttcggg tgccggggat
     3721 cctcatcagc ccgtgggcga aggccggaac gatcgaccac caaacgctct cgttcgatgc
     3781 ctacctcaag ctgatcgagg acctgtacct ggggcgccag cgcctgaacc cgcggaccga
     3841 cgggcgaccc gatccgcgcc ccacggttcg ggagaacgcg aagatcctcg gcgacctgct
     3901 gaaggagttc gacttcagcc agaccccgct cgatccgctg gtgctcgatc cgcgcccgtc
     3961 gccgggaccg gcatccgtcc cgggcggctg agggactgca gcgtccggat cgtcgcttgc
     4021 cggctgggat gggccgctca gccgacgcgg tcgcgcggca cgaaccggcc ccagcccggt
     4081 tcgacgtcgg cgggctcgcc cccccgggcc acgacccgcc cacgcgagac cgttatcgcc
     4141 gcccagccgg tgacggtgag gtcggcgtac gggctgtgat cggtccgcga gtgcaggtcg
     4201 cccgcggcga ggtgcttggt ggccgagggg tcgaagacca ccaggtccgc gtccaggccg
     4261 ggggccaggc ggcccttccg acgggccagg ccgaacagac gcgccggcgc ggcggccacc
     4321 aggtccaccc agcgttcggg tccgaaccga ccgtcgatca cgccctggta cacgagcgag
     4381 agccgggtct ccaccccggg gagcccgccg gggatctggg tgaagtcctt ccagcgagcg
     4441 cccccggcct gggtcccccg ccggcgggtg tcccgggtga aggggcagtg gtcggtggag
     4501 accgcgtcga gctccccgtc ggccagcgct ccccacaggg cctcgcgatc cagttcggta
     4561 cgcagggggg gcacgcagac gaagtcggcc gcctcctgcg agcggccggt caggccggtg
     4621 ccgcggaggg cgtcatcggt caggtacagg tactgggggc aggtctcggc cagcaccagg
     4681 acccggcgcc gtcgggccgt gcggatctcg cgcaggccgg cctcgctgga cagatgcacc
     4741 acgtacaggg acgcggcagc ccgctcggcc agctgcgcgg ccaggcggat ggccgcggcc
     4801 tcgacctcgg gcgggcgggc ctcgatcagg ccggagggac cggtcttacc cgacgccagg
     4861 agccggcggg tccgccggtc ggcctccagc ccgtcttcgg cgtggacgca caccagcacc
     4921 ccggcccgct tgcccgccgc gagggcacgc tccagcgtgg gcgggtcgac catcagtcgc
     4981 tcagggtacg ccaggtacag cttgacgctg gcgacgccct gggccgcgaa ccaccggaac
     5041 agctcctcgg cgtcggccgg atcctcgggg accgaggtga ccgtgaggtg gaacccgtag
     5101 tccaccaccg ctcggccctc ggccttcgcg tgccaggtgc gcagggcctc gtcgggcggc
     5161 tcgcgtccgg cgcccgcgaa gtcgatcacg caggtggtcc caccacaggc ggcggccgtc
     5221 gtgccgctgg cgaagtcgtc ggccgagacc gcggtcccga ccggcaggtc caggtgtgtg
     5281 tgcacgtcga tcgcccccgg gaccaccagc gcgccggagg cgtccaccac cccgtgcccc
     5341 gcgggatcga ggtccggtcc cacggccgcg atgcgctcgc cctcgatcag gacgtctgcg
     5401 cggcgggtgc cggacggttc gacgatggtc ccgcctctga ccagcacgat ggctcaaccc
     5461 cctccggcga tatctctatc atctgggtgt tgaacggggg tggaaccggt gcaactccag
     5521 cgcgacctgg tcgatccggc tgtcctcggc cgcgccgtgg agcggttccg tcgcgacggc
     5581 atcgtgctgc ccaccttcga gcagttggcg gagccggaga agatcccgga gcccatccgg
     5641 gcctcgctga gctccgtgga cccggacgcg ccggaccccc gcaacctgtt ccgggtccac
     5701 tggtacaacg cggccgaccg ccgggggcag gtgccggtcc cgggccacct ggtcctgcct
     5761 ccggagctga cgggggtccc ggcccggatc gtgctcgcgc tgggggaccg gttcccgatg
     5821 atcggcgccc acaaggtcct ggccgcctac ggggtgctcg cgccgaggtt ggtgaccggc
     5881 tcgttcgatc ccacgtccca gcgagcggtg tggccctcca ccgggaacta ctgccggggc
     5941 ggggtggcca tctcccgcat catggactgc cggggcgtgg cggtgctgcc ggaggggatg
     6001 agccgcgagc gcttcgagtg gctggcgcgg tgggtggcgg atccctccga cgtgatccgg
     6061 acccccggca ccgagagcaa cgtcagggag atctacgagg agtgcgctcg gctggagcgc
     6121 gatccggaca acgtgatcct gaaccagttc tcggagttcg ggaaccacct ggtccactac
     6181 ctgtgtaccg gcgctgccct ggagcacgtg ttcgaggcga tgcgcgagga gcagggcgat
     6241 ctgcacctgc gggccttcgt ctcggcgtcc ggctcggcgg ggacgctggg cgccggcgac
     6301 tacctcaggg agcgctacgg ctcgaccacc gtggccgtgg aggcgctcga gtgcccaacc
     6361 ttgctgtaca acgggttcgg cgaacacaac atccagggga tcggcgacaa gcacgtgccg
     6421 ttcatccaca acgtggccgg caccgacgtg gtgacggcgg tctccgatcg tgacaccgac
     6481 cgcctgaacg tccttttcaa cacggaggtg gggcggcggt tcctgcggcg tcgaggcatc
     6541 gcgcccgacg tgatcgccgc gctcgattcc ctgggcctgt cctcgatctg caacgcgctg
     6601 gccgccatca aggtggcccg gtacttcggg atgggccccg acgacgtgat cgtcacggtg
     6661 gccaccgatg gggccgccct gtaccgcacc gaggtggaca aggtcctggc ccgtgacttc
     6721 ggtggctcct ttaacgaggc cgatgccgcg gagacgttcg ggcggtcgtt ggaggccgtc
     6781 accaccgacc acctgctgga gctcacggag cgcgaccgcc ggcgcatctt caacctcggg
     6841 tacttcacct gggtcgagca gcggggggtg tcggtggagg acttcacgct ccggcgctcg
     6901 ccctcgttct ggcgggggct gcgcggtctg ctcccgatct gggacgacat gatcgacgag
     6961 ctgaacgggc gcaccggtct gggggtgggt gggtgaccgc cgagggcacc gccgtggtct
     7021 gctccggctg cgggtaccgg gcgccggccg acgagcccta cccgttccag tgcccgggag
     7081 cgtcctccgg cgacgacatc gatcacctga tggtcagggt cctggacccc gagcacgtga
     7141 gcttcccctg ggacggcgac ccgaacccgt tcacacgctt ccgccggctg ttccacgcct
     7201 accacctcgg tctgcggcac gggatgctgg acgacgagta cgtcgacctg gtccgagacc
     7261 tggaccggcg ggtggccaag gtggacgggc aggggttctc cacgaccccg ttctcgcgtg
     7321 ccgacgagct gtcggagcgt gcggggttcg cgcccgaggg cggcatctgg gtcaaggacg
     7381 agaccggcaa cgtctccggc tcccacaagg cccggcacct gatgggcgtg atgatccacc
     7441 tgctcacgat ggagtggctg agtctcgcgc cctccgatcg gcgccccgac ctggccatct
     7501 cgagctgcgg gaacgcggcg ctggcggccg cggtggtggc gaacgctgcg gggtggccgc
     7561 tccaggtctt cgtgcccgcc tgggccgacc cggcggtgct ggaccggctg gtctcgctcg
     7621 gtgcccgcgt gacgacctgt gagcggcggc ctggggtgag cggcgatccc gcctaccacg
     7681 ccctccgacg cgcgatggac gagggggcgc tgcccttctc cacccagggg cccgacaacg
     7741 gcctgacgat cgaggggggg gagaccctgg cctacgagct ggtgacggac ctggccgccg
     7801 aggatcgggc catggaccgg ctcgtgatcc aggtgggcgg tggggccctc gccagctcgt
     7861 gcatccgggg gttcgaggac gcggccctgc tcgaggcgcc ggtacggctg ccgcggatcc
     7921 acgcggtcca gacggagggt gctcacccgc tggagcgggc cttccgtctg gtccgcgagc
     7981 gcgccggcgc cgagccgggt ccctccgcgg tggtggacgc gatggcctac gccaggaccc
     8041 atcggtccgg gttcatgtgg ccgtgggagt ccgagcccca cagcatcgcc accgggatcg
     8101 tggacgatga gacgtacgat tgggcggcgg tggtgggcgg catgctggcc acggacggcg
     8161 gccccgtgct cgcctccgag gaccgcctcc gcgaggccaa cgacctggct cgaaggacca
     8221 ccgggatcga tgtggatccg accggctccg ccggtctggc gggcgtgctc tcgctgctcg
     8281 aggaggacgc gatcgggccg gccgagcgga tcgccgtgct gttcacgggg gtgcgtcggt
     8341 gagcggcccg ccccctcacc gaccgtcccc cgccgccacg tcgaggaggg cacgatgagg
     8401 agcttcctgg gccgcgacat cctctcgctg aaggacttcg agcgggagga gttcttccac
     8461 gtgttcgaga tctgcgatcg cctggcgccc atcgccaagg agcggcggaa cgccgatctg
     8521 ctgaaggaca agaccctcct cacggccttc taccagccga gcaccaggac ccgcatggcc
     8581 acggaggccg cgatgcaccg cctgggcggg cacgtgctgg ggttctcgga cgccaagatg
     8641 acgcgggccg gcgacttcta ccaggagtcc atcaaggaca cggtccacat gctggagttc
     8701 tacgcggacg tgatcgcgat gcgccacttc cagcagggag ctcccgagga ggccgcacgg
     8761 tgggcttccg tccccgtgat caactgcggc gacggctggg gcgagcaccc cacgcaggtc
     8821 ctgaccgacc tgtacacgac ctggaaggag ctcggccggc tggacggtct gaagttcctg
     8881 ctggtggggg acatgcggat gcgcacgatg cactcgatcc tctacgcctt ctcgcggttc
     8941 gacgtggagt cgttggtggt gtccccgccc gacatgtcgc tgctgccgga gttcaaggcc
     9001 gagctcgagg ggcggagcgt cccgtttcgc gaggtggagt ccgtggagaa gggcattgcc
     9061 gatgcggacg tgatctacat ggagccggtg gtccaggccg actacacgaa gggccgcgac
     9121 gagcgggggg ctgaggtgcc caccaccccc gacgcgtacc gggtcacccg cgagctcctg
     9181 cgggacaagg ggaaggccga ggcgatcgtg ctccactcgc tgccccgcat ggacgagctc
     9241 tcggtcgagg tcgacgccac ccgtcacgcc cggtactggg tggaggcctt caacggggtg
     9301 gtgatgcgga tggcgctgct gtcgctggtg ctcggggccg tcgagtaggg ctcgaccgtc
     9361 gaggcggagg agagtaggca tgcggttcag gctgaacggc cgcccggtgc gggtcgagat
     9421 ccggccgggg atgagcctgc tcgagctgct ccgcgagggg tgcggggtca cctcggtgaa
     9481 ggatgggtgc gcccccgagg gctcgtgcgg cgcctgcacg gtgctggtgg acggcagggc
     9541 ggtggtctcg tgcgcgcagg ccgcaggccg ggccgagggc cgggacgtcc tcacccagga
     9601 tgggctgtcc gaagagcagc ggggcacgtg ggcggacgcc ttcgtggcct ccggtgcctc
     9661 ccagtgcggg ttctgctcgc cggggatcgt cctgaaggcc gagtcgctcc tggccaagaa
     9721 ccctcggccc tcgcgcgagg aggtggcccg agccctcgcc gggaacctgt gtcggtgcac
     9781 cggttacgtg aagatcgtcg acgccgtcga gctggtggcc ggcgctcggc gcggggagcc
     9841 gctgcccgcg gtggacctca gcgggcgggt gggctcgcgt accgctcgct accaggggcg
     9901 cgagctcgcc ctgggcgaca agccctacgt gaacgacctg agcgcgccgg ggatgctgca
     9961 cggggcgatc cgcttctcca accaccctcg ggcgcgcgtg ctccggatcg acgtctcgaa
    10021 ggcccgggcc cacccgggag tgatggcggt ggccaccgcg gctgacgtgc cgggggagcg
    10081 gtcccagggc ctgatcaccc gggactggcg ccagctggtg gccgagggcg aggtcaccgc
    10141 ctacgtcggc gacgtgctgg cggtggtcgc ggcccggagc cggcacgccg cgcgcgaggc
    10201 cgcgcagctg gtcgaggtgg agtacgaggt gctcgagccg gtgaccgacc cccaccgggc
    10261 cctccggccc gaggcgccaa gcgtccacga ggatcgggac aacctgctct cgacctccgt
    10321 cgtgcgacgc ggcgacgcgg aggccgtcct ggcaggatcg acccacgtgc tctccgagac
    10381 gttccagacc caggtgatcg agcacgcctt cctggagccg gagtcggccc tggcggtgcc
    10441 cgaggacggc ggcatgcacg tgtactcgca gggccagggg gtgtgggagg accggcggca
    10501 gatcgcttcg ttcctggggg tggccgaagg ggaggtccgg gtgacccagg tggcgaacgg
    10561 gggtgcgttc ggggccaagg aggacctgaa cgtgcagtgc cacgccgcgc tgctggccca
    10621 tctgacctcc cgcccggtcc tgctcacgct gtcccgccgg gagagcctgg gcttccaccc
    10681 caagcggcac ccgatgacga tgcagtacac gatcggagcc gacgaggagg gccggctcac
    10741 ggcggtccgt gctcggatcg tgggggacac cggggcctat gccagcgtgg gcgacaaggt
    10801 cctggagcgc gccgcagg
//
LOCUS       DASQQQ010000187         3373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_312887, whole genome shotgun sequence.
ACCESSION   DASQQQ010000187 DASQQQ010000000
VERSION     DASQQQ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312887"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2153)
                     /locus_tag="VE646_07345"
     CDS             complement(<1..2153)
                     /locus_tag="VE646_07345"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HZD79838.1"
                     /translation="MHVLAAEGETIKAVFGSFEGRALWVVLVISLIALVFAYYLVREV
                     LASLEGTTKMRDIARAIQEGARAYLNRQFKTIAVFIGVLTVLLFFVLPVAKDSVHGDL
                     FIRFGRSLAFIVGAGFSAIAGYTGMWLAVRANVRTANAARESGLRRALRIAFRAGGVA
                     GMFTVGLGLLGATIIMMIYKRDATGVLIGFGFGGSLLAMFMRVGGGIFTKAADVGADL
                     VGKVEKNIPEDDPRNAAVIADNVGDNVGDCAGMAADLFESYEVTLVAALILGAAAFQN
                     SPKGAIIGVMFPLFVRAVGVITSIVGILLVSPRREDEPGMRAINRGFFISAALSAAIV
                     FAISEMYMNTLRPGFAVLFGLILASVIQVLTEHFTSTRRHPVREIAEQTQTGPATTIL
                     SGFSVGLESTVWAMLVIGGSIIAAFLIGRTTAERLYYISLTGMGMLTTVGVIVSMDTY
                     GPVSDNAQGIAEMTGEFEGRPGEILTGLDAVGNSTKAITKGMAIATAVLAATSLFGSF
                     EEALAGAGFHFLGIRVDRPDVLVGLLIGGSVAFLFSSLAIRAVGRAASQVVVEVRDQF
                     RHHPGIMEFKEKPDYARVVDICTRTSLRELLTPGLLAVLSPIIVGFALGGEPLGAFLA
                     GAILTGQLLAVMLSNAGGAWDNAKKFVEEGHYGGKGSEAHKASVIGDTVGDPFKDTAG
                     PALNPMIKVMNLVSLLIAPLVVEYADKDWARVGIAV"
     gene            2465..>3373
                     /locus_tag="VE646_07350"
     CDS             2465..>3373
                     /locus_tag="VE646_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007298620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZD79839.1"
                     /translation="MAVRTRGARKRHDRAVKATGWWALLLAFSFAVMADPASSVAYAI
                     EAGLRALHGHLHLLLLTMGLVLGIISLVRLNYDQLIRRFPQGGGAVAATAVAFGEGWA
                     FWPLGALVVDFVLTIAISAAAASSAVIAYFPRLAPERAGIALLLVCLVAGLTWFGHLG
                     RSVFAWMTATFIAVALVIFAAGFLAPHTRVFDPQPVTDGSAGFLTVLLAFPVAMALAT
                     GVEAPSSAIAQLGQLDDRGRERFGRVTLWLTFVVEGSLTAFIAVLAVHLHVGIPGHDS
                     TLIADVAEASVGRGALFALFQLTSSLL"
BASE COUNT          545 a         1155 c         1149 g          524 t
ORIGIN      
        1 accgcgatgc ccaccctggc ccagtccttg tcggcgtact cgaccaccaa cggcgcgatc
       61 aggagggaca cgaggttcat caccttgatc atcgggttca gcgccggacc ggcggtgtcc
      121 ttgaacggat cccccaccgt atccccgatc acagaggcct tgtgggcctc gctgcccttg
      181 cctccgtagt ggccctcttc cacgaacttc ttcgcgttgt cccaggcacc gccggcgttg
      241 gagagcatga ccgccaggag ctgtccggtg aggatggcgc cagccaggaa tgcccccagg
      301 ggctctcccc ccagggcgaa gcccacgatg atgggggaca ggacggccaa caagcctggt
      361 gtgagcaact cgcggagcga ggtccgggtg cagatgtcca ccacccgtgc gtagtcgggc
      421 ttctccttga actccatgat gcccgggtgg tggcggaact ggtcccgaac ctccaccacc
      481 acctgcgacg cggccctgcc aaccgcgcgg atggcgagcg acgagaacag gaacgcgacc
      541 gagcctccga tgaggagccc caccaacacg tcgggtcggt cgaccctgat gcccaggaaa
      601 tggaacccag cgcccgccag cgcctcctcg aacgagccga acaaggaggt cgcggcgagc
      661 acggcggtgg cgatggccat ccccttcgtg atggccttgg tcgagttccc gaccgcatcc
      721 agtcccgtga ggatctcgcc cggccggccc tcgaactcgc cggtcatctc ggcgatgccc
      781 tgcgcgttgt ccgacaccgg tccgtacgtg tccatcgaga cgatgacgcc caccgtggtg
      841 agcatgccca tgccggtgag ggagatgtag tagaggcgct cggcggtggt cctgccgatg
      901 aggaacgcgg cgatgatgga cccgccgatc accagcatgg cccacaccgt ggactccagc
      961 cccaccgaga atcccgacag gatggtggtg gcggggccgg tctgggtctg ctcggcgatc
     1021 tcccgcaccg gatgccggcg ggtcgacgtg aagtgctcgg tcaggacctg gatcacgctg
     1081 gccaggatca ggccgaagag gacggcgaag ccgggccgca acgtgttcat gtacatctcc
     1141 gagatggcga acacgatggc cgccgaaagg gccgcggaga tgaagaagcc ccggttgatg
     1201 gcccgcatgc ccggctcgtc ctcgcgacgt ggcgagacga gcaagatgcc cacgatcgag
     1261 gtgatgacgc ccaccgcccg cacgaagagg gggaacatca ccccgatgat ggctcccttg
     1321 ggcgagttct ggaacgccgc agcaccgagg atcagcgctg cgaccagggt gacctcgtaa
     1381 gactcgaaca ggtcggccgc catcccggcg cagtcgccga cgttgtcgcc cacgttgtcg
     1441 gcgatcaccg cagcgttgcg cgggtcgtcc tcggggatgt tcttctcgac cttgcccacc
     1501 aggtcggccc ccacgtcggc ggccttggtg aagatgccgc cgcccacccg catgaacatc
     1561 gcgaggagcg agccgccgaa gccgaagcca atcaggacgc cggtcgcgtc ccgcttgtag
     1621 atcatcatga tgatggtggc gcccagcaag cccaggccta ccgtgaacat cccagcgacg
     1681 ccgccggcac ggaaggcgat ccgcagagcc cggcgaaggc ccgactcccg ggcggcattg
     1741 gccgtccgga cgttggcgcg aaccgccagc cacatcccgg tgtagccggc gatggccgag
     1801 aacccagcgc cgacgatgaa ggccagcgag cgaccgaagc ggatgaacag gtcgccgtgc
     1861 accgagtcct tggccaccgg cagcacgaag aacagcagca cggtcaggac gccgatgaag
     1921 accgcgatcg tcttgaactg tcgattcagg tatgccctgg ccccctcttg gatggcccgg
     1981 gcgatatccc gcatcttcgt ggtgccctcg agggacgcca ggacctcccg tacgaggtag
     2041 taggcgaaga cgagcgcgat cagggagata acgaggacca cccacaacgc ccggccctcg
     2101 aacgagccga agacggcctt gatggtctcg ccctccgcgg ccaggacgtg catgagacct
     2161 ccccggtacc gatgacggaa agcgtgcagg tatcagccga aacgcagggg attataggag
     2221 gaacgcacgg ggctcggctc gggaccgacc gtccccccct cggggggcga tctggtcgcg
     2281 ggcagggccg atccatcggc cccaggggag accgtcggga cccggtagta cgatgggtgg
     2341 cggctggcaa cgtcgtccgg tggatcatcg gtccgaacga tgcgccgcaa cagatgcaca
     2401 ggggtgccgg gaagcctggt cggcgatccg gtgcccttcg tcgtgtctgg aggccctcga
     2461 gcggatggcg gtgcgaacgc gtggtgcgcg caaacggcac gaccgtgccg tcaaggccac
     2521 cggctggtgg gcgctgctgc tggccttctc cttcgcggtc atggcggacc cggcgtcgtc
     2581 ggtggcatac gcgatcgagg cggggctgcg cgcgctccac gggcacctgc atctcctgct
     2641 cctcacgatg gggctggtcc tcgggatcat ctcgttggtc cggctcaact acgatcagct
     2701 gatccgtcgg ttccctcaag ggggcggtgc ggtagcggcc accgcggtgg ccttcgggga
     2761 gggctgggcg ttctggccgc tcggagcgct ggtggtggac ttcgtgttga ccatcgccat
     2821 cagcgccgcc gccgcctcct cggcggtgat cgcctacttc ccgcggctgg cgcccgaacg
     2881 agccgggatc gccctgctgc tcgtctgcct ggtggccggc ctcacctggt tcgggcacct
     2941 gggccggtcg gtgttcgcct ggatgaccgc gacgttcatc gccgtggcgc tggtgatctt
     3001 cgccgccggg ttcctcgccc cccacacgcg ggtgttcgac ccgcagccgg tcaccgatgg
     3061 gtcggcgggc ttcctcaccg tgctgctggc cttcccggtg gcgatggcgc tggccaccgg
     3121 ggtggaggcg ccgtcctcgg ccatcgccca gctcggccag ctcgacgacc gcggccgcga
     3181 gcggttcggt cgggtcacgc tctggctcac gttcgtggtg gagggatcgc tcaccgcgtt
     3241 catcgcggtg ctggccgtcc acctccacgt ggggattccc gggcacgaca gcaccctgat
     3301 cgccgacgtg gccgaggcct ccgtgggacg gggcgccctg ttcgccctgt tccagctcac
     3361 cagctcgctg ctc
//
LOCUS       DASQQQ010000188         4044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_287642, whole genome shotgun sequence.
ACCESSION   DASQQQ010000188 DASQQQ010000000
VERSION     DASQQQ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287642"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VE646_07355"
     CDS             complement(<1..1098)
                     /locus_tag="VE646_07355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZD79840.1"
                     /translation="MNDGTPTILYTSADPDTHGGAFRWLVQMTTEIGDRGFGSVLALS
                     RGTGSAGAGTRIPTHLLSLPRPRRGRAPLQYAGDVVQTIRSARALARIIRREGVALVH
                     ANEILDVYAGLAARLAGVPCVWHVRADISSWPAPLRRTLPRVAAGLASRVVVVSGSVR
                     QHVFIEQGVPTRKVSVIHDAGPDPRTFHPGVDGASVRAELGVPADGSLVVLVSKLVEP
                     KGHEVLVRAVPRVLGSFPGTRFLIVGGEMEGEHHRRYAERLRRLPAQLGVAEAVTFAG
                     YRDDVPQVMAAADLIAHCSTHPDPFPGVVLQGMALGRPVIATDLGGATEQIEDGLSGV
                     LVPPGDPLALGEAITSLLKDPDRRASLGAAAV"
     gene            complement(1095..2336)
                     /locus_tag="VE646_07360"
     CDS             complement(1095..2336)
                     /locus_tag="VE646_07360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZD79841.1"
                     /translation="MSLGSAGTDPRRSGGAVGAVADATRPRRVLCVFGSFERAGAQLR
                     TLEVLRELRRDRLVQFAFCSLDLGPDEVSPDVAEAGGSVHRVSIRSPRFLVAFPRLLR
                     RGRYDVVHTEPQFLSGIVVWLAALCRVPVRIVAIHNSVGDAGQTARKRWVRLVLANRV
                     FLWSMRTLTSRYATNVIAVSASALDSVLPGRWQARRDCRVVYNGTDLGPFRRPEDRVG
                     VRREFGWPDDARIVVNVGRLSTQKNHPVILEAIRRLREEDPRVRLLLVGGGKLRDRIE
                     ELVDRLGLRDACAIVENRPDVPRLLLASDVFFFPSAWEGLPGSPLEALAAGLPIVSSD
                     IPPMLEISPHFPGSILVAPPDDVEAHVEHLRAALRMPRERERVQERFAGSPFALPQAV
                     VAYRGLYRVRGPAPARAEARA"
     gene            complement(2333..3895)
                     /locus_tag="VE646_07365"
     CDS             complement(2333..3895)
                     /locus_tag="VE646_07365"
                     /inference="COORDINATES: protein motif:HMM:NF025654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="HZD79842.1"
                     /translation="MATTFLPSRADRRRKDLAYREQLRIVLLFLAVPATLGLALLVAN
                     GEPLPLPDLRISALTTVVFCLVAIALVLYVHKLHLLSFPSLFLAVTFLFTCSPLILYL
                     LQGERAFRLWEVVDYPAVLAAMPVIMLAFSSFLLGAVLIHVRLPADAPAATEEEGRPP
                     SPSATTRVLRGVGFGLYGVGFLFVAYSTISGGTLSYALSGGYAAYHEAKRSGAIVGFV
                     GVFMAHLLPWSLLILTATARDRRSRALVLVLAAPFVLVMLAVGDRGGPIATMAIVASG
                     LYLVGARIGLGKSLAVGLLIAFLIPTILNLRQLPISEWTGTAIGSAATNQVDVTNTYG
                     EGPITGFFTSMSSPYQTLMATVAQVPQHEDYHHGSDYLSSMVVAIPFRSVLFPFFGAE
                     VNRLPPSQWVLLLLHPGRNAGPGYLQVAEGYLQFGAVGVIGLYLLFGWLLIRLWGAMT
                     RRVHDPRVLAFTLIVMMETLLWVRNSSSSVVRAIAWGAVLVFVVPAVLTVRRWRRSEQ
                     PTPPGPITVLHR"
BASE COUNT          656 a         1460 c         1471 g          457 t
ORIGIN      
        1 gaccgcggcg gcgccgagcg aggcccgccg gtccgggtcc ttgagcagcg aggtgatcgc
       61 ctcccccagg gccagggggt cgcccggagg gaccaggacc ccggagaggc cgtcctcgat
      121 ctgctcggtg gcgccgccca ggtcggtggc gatcaccggc cgacccaggg ccatcccctg
      181 caggaccacg ccggggaacg gatcggggtg ggtcgagcag tgcgcgatca ggtcggccgc
      241 ggccatcacc tgcggaacgt cgtcccggta gccggcgaag gtcacggcct cggccacgcc
      301 gagctgggcc ggcagtcgcc ggagccgctc cgcgtatcga cgatggtgct cgccctccat
      361 ctccccgccc acgatcagga agcgggtccc cgggaacgac ccgagcaccc gggggacggc
      421 ccggaccagg acctcgtgcc ccttgggctc gaccagcttg gagaccagga ccaccagcga
      481 gccgtcggcg ggcaccccga gctcggcccg gaccgatgcg ccgtccacgc ccggatggaa
      541 ggtccgggga tcgggcccgg catcgtggat caccgagacc ttccgggtcg gcacgccctg
      601 ttcgatgaac acgtgctgcc gcaccgaccc cgagaccacc accacccggc tggccaggcc
      661 ggccgccacc cggggcaggg tcctgcggag cggagcgggc caggacgaga tgtcggcccg
      721 gacgtgccag acgcagggga cgccggccag gcgggccgcg agccccgcgt agacgtccag
      781 gatctcgttg gcgtggacga gggccacgcc ctcccgacgg atgatccggg ccagggcgcg
      841 ggcgctgcgg atggtctgca ccacgtcgcc cgcgtactgc agcggggccc tcccccgccg
      901 gggacggggc agggacagga ggtgcgtggg gatccgggtc ccggcccccg cggagccggt
      961 ccctcgggac agggccagga cggagccgaa ccctcggtcg ccgatctcgg tggtcatctg
     1021 caccagccac cggaaggcgc cgccgtgcgt gtcgggatca gccgaggtgt agaggatcgt
     1081 gggcgttccg tcgttcatgc tcgagcctcc gcgcgcgcgg gggccggccc gcgcacccgg
     1141 tacagccccc ggtacgccac caccgcctgc ggcaacgcga acggggaccc cgcgaaccgc
     1201 tcctgcaccc gctcacgctc gcgcggcatc cgcagggccg cccgcaggtg ctccacgtgg
     1261 gcctccacgt cgtcgggggg cgcgaccagg atcgatccgg ggaagtgggg ggagatctcg
     1321 agcatgggcg ggatgtcgct ggacacgatc ggcaggccgg cggccagcgc ctccaggggc
     1381 gacccgggca ggccctccca ggccgagggg aagaagaaga cgtcggaggc cagcagcagc
     1441 cgggggacgt cggggcgatt ctccacgatc gcgcaggcat cgcgcaggcc caggcgatcg
     1501 acgagctcct cgatccggtc ccggagcttg ccgcccccca ccagcagcag ccggacccgg
     1561 gggtcctcct cgcgcaggcg ccggatcgcc tccaggatca ccgggtggtt cttctgggtg
     1621 gacagccgcc ccacgttcac cacgatccgg gcgtcgtccg gccagccgaa ctcccgccgg
     1681 acgcccaccc gatcctcggg tcggcggaag gggcccaggt cggtgccgtt gtagaccacc
     1741 cggcagtccc gccgggcctg ccaccgcccc ggcagcacgc tgtccagcgc gctggcggac
     1801 acggcgatca cgttcgtggc gtaccggctc gtgagggtcc gcatcgacca caggaacacc
     1861 cggttcgcca ggaccaggcg gacccagcgc ttccgcgcgg tctgccccgc atcgcccacc
     1921 gagttgtgga tcgccacgat gcgcaccggc acgcggcaca gcgcggccag ccacaccacg
     1981 atcccgctca ggaactgcgg ctcggtgtgg accacgtcgt agcggccgcg ccggagcagc
     2041 ctggggaacg caaccaggaa gcggggggag cggatcgaga cccggtggac ggacccgccc
     2101 gcctcggcca cgtccgggct gacctcgtcc gggccgaggt ccagggaaca gaaggcgaac
     2161 tgcaccagcc ggtcgcgacg cagctcgcgc aggacctcca gggtccgcag ctgcgccccg
     2221 gcccgctcga agctcccgaa cacgcagagc acccgccgcg ggcgcgtcgc atccgccacc
     2281 gcgccgaccg cccctccgga gcgacgagga tcggtcccgg ccgaccccag gctcatcggt
     2341 gcagcaccgt gatcggtccg ggtggggtcg gttgctccga gcgtctccac cgccgcaccg
     2401 tgagcacggc cgggaccacg aacaccagga cggcgcccca ggcgatcgcg cgcacgaccg
     2461 aggaggagga gttccgcacc cacagcaggg tctccatcat cacgatcagg gtgaaggcca
     2521 gcacgcgggg atcgtgcacc cggcgggtca tggcgcccca caggcggatc agcagccacc
     2581 cgaacagcag gtacaggccg atcaccccga cggcgccgaa ctgcaggtac ccctcggcca
     2641 cctgcaggta gcccggaccg gcgttgcgcc cggggtggag caggagcagc acccactggc
     2701 tcggcgggag acggttcacc tcggcgccga agaaggggaa caggaccgag cggaacggga
     2761 tcgccaccac catggagctc aggtagtcgc tgccgtggtg gtagtcctcg tgctgcggca
     2821 cctgggccac cgtggccatg agcgtctggt aggggctgga catcgaggtg aagaagccgg
     2881 tgatgggtcc ctcgccgtag gtgttggtga cgtccacctg gttggtggcc gcgctcccga
     2941 tcgcggtgcc cgtccactcg gagatcggca gctgtcgcag gttcaggatg gtggggatca
     3001 ggaacgcgat cagcagcccc acggccaggc tcttgcccag gccgatcctg gcccccacca
     3061 ggtacagccc cgaggccacg atggccatgg tggcgatggg gccgccgcga tcgcccaccg
     3121 ccagcatcac caggacgaac ggcgccgcga gcaccaggac cagcgcccgg gaccggcggt
     3181 cgcgggcggt cgcggtgagg atcagcaggg accaggggag caggtgcgcc atgaacacgc
     3241 ccacgaaccc gacgatggcg ccgctccgct tcgcctcgtg gtaggcggcg tagccgccgg
     3301 acagcgcgta gctgagggtc ccccccgaga tcgtggagta ggccacgaac aggaagccca
     3361 cgccgtacaa cccgaaccca accccacgca gcacccgggt ggtggcgctg ggtgagggcg
     3421 gccgcccttc ctcctcggtc gccgcgggcg catcggccgg caggcggacg tggatcagca
     3481 ccgcccccag gaggaaggag gagaacgcca gcatgatcac cggcatcgcc gcgagcacgg
     3541 ccgggtagtc caccacctcc cacaagcgga aggcccgctc cccctggagc aggtagagga
     3601 tcagggggct gcacgtgaac aggaacgtga ccgccaggaa cagcgaaggg aacgagagca
     3661 ggtgcagctt gtgcacgtac aggaccaggg cgatggccac caggcagaac accacggtgg
     3721 tcagcgcgga gatccgcagg tcgggcaggg gcaacggctc gccgttcgcc accagcaggg
     3781 ccaggcccag ggtggccgga accgccagga acaggagcac gatccgcagc tgctcccggt
     3841 aggccaggtc cttcctccgg cggtcggcgc gggagggaag gaacgtggtc gccatctcgc
     3901 cgtcaggtga ccgtgaagtc cgtcgagctg gtggccgcgc ccagggcgtc cttcacctta
     3961 atcggcccgg tggtcgtgtt gggcacggtc gcggtgatct ggctgtccga gtcgatggtg
     4021 aaggcggcct tgaccccgtt gaac
//
LOCUS       DASQQQ010000189         5647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_124842, whole genome shotgun sequence.
ACCESSION   DASQQQ010000189 DASQQQ010000000
VERSION     DASQQQ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124842"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VE646_07370"
     CDS             <1..695
                     /locus_tag="VE646_07370"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZD79843.1"
                     /translation="LGTSGFAYDGWKHGVFYPESLKNADMLAYYASRFRSVEVNYTFR
                     RFPSEKTLTTWRAATPEGFAFTLKANQRITHVKRLADADEDVSTFLNRARLLGDRLGT
                     VLFQCPPSLRYDRQLIERFVGGLPPDVRAVMEFRHGSWVEARDFLHEQGIAWCVAETD
                     DRDPGPEDLSWEPYGYLRLRKTSYEHDELRSWAGRIGPALGSGADVYCYFKHEDEGLS
                     PRLAERLDALIR"
     gene            complement(715..3189)
                     /locus_tag="VE646_07375"
     CDS             complement(715..3189)
                     /locus_tag="VE646_07375"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HZD79844.1"
                     /translation="MDAAPVERLLDELRSPDRAYSTGHHGRWSASRRAALVDACLRDL
                     FMDVAGEGLALVAQGGYGRSELAPGSDLDLLILHSARAGDEVASLTEGLLYPLWDAGL
                     TVGHAVRTPGECLEVARERVDALTAMLEGRIVAGSSSLWDEVHEGLVRSVRESPRAFA
                     LELRDEARSRRERHGSVSWLLEPELKEGAGGLRDVHSLGWLAVAVADHPGLAGLMSQG
                     LVRDAERLAVEAAWEFLVRVRSALHLETGRPTDRLLLDHQPPVARAMGFEDEPGLRAV
                     DGLMRTVFEHARQVEHVRRAAFGRFLRGGASDRRLEPTPEGVLSVFALAAAEGGVLGA
                     SELDRIDAVRLPEPIAWTAPVLDRFLQILRSGRDGVRALEAMDRIGLLVRFLPEWGPV
                     RCRPQRDPYHRYTVDVHLLQALAGMAELVGPEPPSEPLVVEAAAAVTDRDALLLGAFL
                     HDIGKSGQGSHVPVGARVAATALDRMDLPPATRDLAWFLVAEHLLLSDTATRRDLEDD
                     DLILDVAARIGTPERLAALYLLAVADAGATGPAAWTPWRATLIRELVSKVQRVLERGD
                     VGPETAERLTVRADALRERLAEEEPVAIDRFLLRMPRSYFLSVPLERIVEHYPLVASP
                     VGAAEVRTLALPGSRPGTYAVTVVAADRPGLLSWIAGALSLAGLSILTAQVFTTQDGS
                     AVDVFEVEGVFEPEVGEDRWREFRTTLRRAIEGRLSLEYRVERKRAFYPEPRVHRPVT
                     VTLDNGVSDLFTVIEVGAPDRIGLLFDVTRTLAELHLDVHLAKVATYAGRVIDAFYVR
                     DELGRKVIEPSQIEEVRGALIARVRP"
     gene            complement(3209..3547)
                     /locus_tag="VE646_07380"
     CDS             complement(3209..3547)
                     /locus_tag="VE646_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019064219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZD79845.1"
                     /translation="MKLVVAIVKPFKLDEVKETLRELGVHGLTTTEVEGFGRQRGHTE
                     VYRGAEYQVDFVPKVKVEVVCDDDQVQEVVEGVMKAARTGKIGDGKIWVTPAEQVIRI
                     RTGEMGPDAL"
     gene            complement(3605..4456)
                     /locus_tag="VE646_07385"
     CDS             complement(3605..4456)
                     /locus_tag="VE646_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HZD79846.1"
                     /translation="MWKGAISFGLVTIPVAVFPATEEKSLRFNQLHDEDMGRIRYKRV
                     CEKDGEEVSFDHIVKGFEYEKDRYVVLTDEDLDKVPVESSRAIDIQQFVDLDEIDPIL
                     FKKSYYLVPEETGAKAYSLLRRALSDESKVGIAKVAFRDKEHLAALRFKDEVFVLETM
                     YWPDEVRAADFDTLSTDGTVRPQEVEMAKTLIENLTEPWNPESFRDEYREGLQEIVDK
                     KVAGEEIEEVPEAAPSRVVDLMEALKASVEAAKKKTPPSAAKPSGAKKSAAKKKSTAP
                     RKKAAAG"
     gene            4590..>5647
                     /gene="ligD"
                     /locus_tag="VE646_07390"
     CDS             4590..>5647
                     /gene="ligD"
                     /locus_tag="VE646_07390"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HZD79847.1"
                     /translation="MPGKRRSRSGPAFTVAFPSTLPVERGDGDAWWTEVAGRELRLSN
                     LDKVFWPDEGYTKGDLIAYYYNVAELILPHLLERPLTMKRMPDGLSGGFFYEKSAPSH
                     TPEWLPRCRVRSEDAKDGLIDYLMVEDLAGLLFVANLGCIEFHPLHSRCPSVDSPDYL
                     FFDLDPFEPITFGDVLAVAMHVKTALDSLGVTGYPKTSGATGMQIYVPIDPGYGYQQV
                     RDLVGAVGRTIQRADPERVTMAWQISRRAGKVFIDHNMNRSGANIAAVYSLRPEAGAT
                     VSTPLTWDEVEAGVAPGDFTIASVWSRFARVGDLFEGVRTEPQDLGSVLDAFALPSDR
                     EHGSPPRTSEDVIGRSRDP"
BASE COUNT          832 a         2126 c         1860 g          829 t
ORIGIN      
        1 acctgggcac ctcggggttc gcctacgatg gctggaagca cggcgtgttc tacccggaga
       61 gcctgaagaa cgccgacatg ctcgcctact acgcgagccg gttccgctcg gttgaggtga
      121 actacacgtt ccgtcggttc ccctccgaga agaccctcac cacctggcga gcggccacgc
      181 ccgagggctt cgccttcacg ctgaaggcga accagcggat cacccacgtc aagcggctgg
      241 ccgacgcgga tgaggacgtg agcacgttcc tgaaccgggc ccggctcctg ggcgaccgcc
      301 tgggcacggt gctgttccag tgcccgccct cgctcaggta cgaccggcag ctgatcgagc
      361 ggttcgtggg ggggctcccc ccggacgtcc gcgcggtgat ggagttccga cacgggtcgt
      421 gggtcgaggc tcgcgacttc ctacacgagc agggcatcgc ctggtgcgtg gccgagaccg
      481 acgaccgtga tcccggaccc gaggacctct cctgggagcc gtacgggtac ctgcgcctgc
      541 ggaagacctc ctacgagcac gatgagctgc ggagctgggc cgggcgcatc ggccccgcgc
      601 tcggatcggg cgccgacgtc tactgctact tcaagcacga ggacgagggt ctgagcccac
      661 ggctcgccga gcgcctggac gccctgatcc ggtaacgcct cgcggaccgt gcgcctaggg
      721 gcgcacgcgg gcgatgagcg cgcccctcac ctcctcgatc tgcgagggct cgatcacctt
      781 gcgaccgagc tcgtcccgca cgtagaaggc gtcgatgacc cgaccggcgt acgtggcgac
      841 cttggccagg tgcacgtcca ggtgcagctc cgccagggtc ctggtcacgt cgaacagcag
      901 gccgatgcgg tcgggcgcgc cgacctcgat cacggtgaag agatcggaca cgccgttgtc
      961 cagcgtcacg gtcacgggcc tgtgcacccg cggctccggg tagaaggcgc gcttgcgctc
     1021 gacccggtac tcgagcgaca gccgaccctc gatcgcgcga cggagcgtgg tgcggaactc
     1081 ccgccaccga tcctcgccga cctccggctc gaagaccccc tccacctcga acacatccac
     1141 ggcggacccg tcttgggtgg tgaagacctg ggccgtgagg atcgataggc cggccaggga
     1201 gagggccccg gcgatccacg acagcaggcc ggggcggtcg gcggccacca ccgtgaccgc
     1261 gtaggtgccg ggacgcgagc ccgggagcgc cagcgtgcgg acctccgccg cccctacggg
     1321 cgaggccacc agcgggtagt gctcgacgat ccgctccagc ggtacgctca ggaagtagct
     1381 ccgaggcatc cgcagcagga accggtcgat cgccacgggt tcctcctccg ccaaccgctc
     1441 ccggagcgcg tcggcccgca cggtcagtcg ctcggcggtc tcgggtccca cgtcgccccg
     1501 ttccaggacc cgctggacct tggacaccag ctcgcggatc agcgtggcgc gccacggggt
     1561 ccatgcggcc ggcccggtgg caccggcgtc ggccaccgcg agcaggtaca gggcggccag
     1621 acgctccggc gtcccgatcc gggccgccac gtccaggatc aggtcgtcgt cctccaggtc
     1681 ccgccgggtg gccgtgtcgg agagcagcag gtgctcggcc accaggaacc acgccagatc
     1741 ccgcgtggcg ggcgggaggt ccatccggtc cagggccgtg gccgccaccc gcgcccccac
     1801 cggcacgtgc gacccctgcc ccgacttccc gatgtcgtgc aggaacgcgc ccagcagcag
     1861 cgcgtcgcga tcggtgaccg ccgccgccgc ctcgaccacc agcggctccg agggcggctc
     1921 cgggccgacc agctcggcca tccccgccag cgcctggagc agatggacgt ccaccgtgta
     1981 gcggtggtag ggatcgcgct ggggccggca acggaccggc ccccactcgg gcaggaaccg
     2041 gaccaacagg ccgatgcggt ccatcgcctc cagagcccgg accccatccc tgcccgagcg
     2101 caggatctgc aggaaccggt ccagcaccgg cgccgtccag gcgatcggct ccgggagccg
     2161 aaccgcgtcg atccggtcca gctccgaggc acccagcacg ccgccctcgg cggcggccag
     2221 cgcgaacacc gacaggacgc cttcgggcgt gggctcgagg cgccgatcgc tcgccccgcc
     2281 ccgcaggaac cgcccgaacg cggcccggcg cacgtgctcc acctgacgcg cgtgctcgaa
     2341 caccgttcgc atcaacccat ccaccgcacg cagccccggc tcgtcctcga agcccatggc
     2401 ccgggccacc gggggttggt gatccagcag cagccggtcg gtggggcgcc ccgtctccag
     2461 gtgcagggcg ctccggaccc ggaccaggaa ctcccacgcc gcttccacgg ccagccgctc
     2521 cgcgtcccgg accaacccct gggacatcaa gccggccaga ccgggatggt cggccacggc
     2581 caccgccagc catccgaggg agtggacgtc ccgcagcccc ccggctcctt ccttcagctc
     2641 aggctccagc agccaggaga cggacccgtg gcgctcgcgg cgactccggg cctcgtcccg
     2701 aagctcgagc gcgaacgctc gcggagactc gcggaccgat cggaccagtc cctcgtggac
     2761 ctcgtcccac agcgagctcg agcccgccac gatccgtccc tccagcatgg ccgtcagcgc
     2821 gtcgacgcgc tcccgcgcta cctccaggca ctccccgggc gtccggaccg cgtgccccac
     2881 ggtgagcccc gcgtcccaca gcgggtacag gaggccctcg gtcagcgatg cgacctcgtc
     2941 gccagcgcgg gccgagtgga ggatcagcag gtccaggtcc gatcccggtg ccagctcgga
     3001 tcgtccgtag cccccttggg cgaccagcgc gagcccctca cccgcgacat ccatgaacag
     3061 gtcgcggagg cacgcgtcga ccagcgcggc ccgccgcgag gccgaccacc ggccgtggtg
     3121 gccggtcgag taggcccggt ccggggaccg cagctcgtcc aggagccgct ccacgggcgc
     3181 ggcgtccacc cgcatctccg ccccgcgatc agagggcgtc ggggcccatc tccccggtgc
     3241 ggatgcggat cacctgctcg gcgggcgtca cccagatctt cccgtcgccg atcttgccgg
     3301 tgcgagccgc cttcatgacc ccctcgacga cctcttgcac ctggtcgtcg tcgcagacca
     3361 cctcgacctt caccttgggc acgaagtcca cctggtactc cgccccccgg tacacctccg
     3421 tgtggccgcg ctggcggccg aagccctcga cctcggtggt ggtgagcccg tggacgccga
     3481 gctcccgcag cgtctccttc acctcgtcca gcttgaaggg cttcacgatg gccacgacca
     3541 gcttcacggc acaggacctc ctccagggcg gccccagctt agccagcggt ggggcgcgaa
     3601 gtgctcagcc ggcggcggcc ttcttcctcg gcgccgtgga cttcttcttc gccgccgact
     3661 tcttggcccc cgacggcttg gccgcggacg gcggcgtctt cttcttggcc gcctccaccg
     3721 acgccttcag cgcctccatc agatccacca ccctggaggg cgcagcctcg ggcacctcct
     3781 cgatctcctc gccggcgacc ttcttgtcca cgatctcctg gaggccctcg cggtactcgt
     3841 cccggaacga ctccgggttc cacggctcgg tgaggttctc gatgagcgtc ttggccatct
     3901 cgacctcctg agggcggacc gtgccgtcgg tgctgagggt gtcgaagtcg gcggcccgta
     3961 cctcgtcggg ccagtacatc gtctccagga cgaagacctc gtccttgaac cgcagcgcgg
     4021 cgaggtgctc cttgtcgcgg aaggccacct tggcgatccc caccttgctc tcgtcgctca
     4081 gcgcccggcg gagcagcgag taagccttgg cccccgtctc ctcgggcacg aggtagtagg
     4141 acttcttgaa gaggatcggg tcgatctcgt cgaggtcgac gaactgctgg atgtcgatgg
     4201 cccggctgga ctccaccggg accttgtcca ggtcctcgtc ggtcaggacc acgtagcggt
     4261 ccttctcgta ctcgaagccc ttcacgatgt ggtcgaagga gacctcctcc ccgtccttct
     4321 cgcacacgcg cttgtaccgg atccggccca tgtcctcgtc gtggagctgg ttgaagcgaa
     4381 gggatttctc ctcggtggcg gggaacacgg ccaccgggat cgtgaccagg ccgaaggaga
     4441 tggctccctt ccacatcgct ctgggtggca tggcatgacc tccaggggtc ggtcgatctc
     4501 agccgaaagg tcttatccta gcggcccggg gccccaagaa cgagcaggtg agggccgtga
     4561 tggctctccc gcgcggctac catcgaggga tgccggggaa acgcagatcc cgctcgggcc
     4621 ccgccttcac cgtcgccttc ccctccaccc tgcccgtcga gcggggcgac ggcgacgcct
     4681 ggtggaccga ggtggcgggc cgcgagctcc gcctgtccaa cctggacaag gtcttctggc
     4741 cggacgaggg ctacacgaag ggcgacctga tcgcctacta ctacaacgtc gcggagctga
     4801 tcctcccgca cctgctggaa cggcccctca cgatgaaacg gatgccggac gggctgtccg
     4861 ggggcttctt ctacgagaag agcgcgccct cgcacacgcc cgagtggctc cctcgttgcc
     4921 gggtccgctc cgaggatgcc aaggacggcc tgatcgacta cctgatggtc gaggacctgg
     4981 ccggcctgct gttcgtggcc aacctggggt gcatcgagtt ccaccccttg cactcgcgct
     5041 gcccctcggt ggactctccc gattacctgt tcttcgacct ggaccccttc gagcccatca
     5101 cgttcgggga cgtcctggcc gtggccatgc acgtcaagac cgcgctggac tcgctcggcg
     5161 tgaccggcta cccgaagacg agcggcgcca ccgggatgca gatctacgtc cccatcgacc
     5221 ccggctacgg ctaccagcag gtccgcgacc tggtcggcgc ggtgggccgc acgatccagc
     5281 gggccgaccc cgagcgcgtg acgatggcct ggcagatctc ccgccgggcc gggaaggtgt
     5341 tcatcgatca caacatgaac cgctccggcg cgaacatcgc cgccgtctac tccctccggc
     5401 ccgaggccgg ggccaccgtc tccacgccgc tcacctggga cgaggtcgag gccggtgtgg
     5461 caccggggga cttcacgatc gccagcgtgt ggagccggtt cgcgcgcgtg ggcgatctgt
     5521 tcgagggggt ccgcacggag ccccaggacc tgggctcggt cctcgatgcg ttcgccctgc
     5581 cctcggaccg cgagcacggg agtcctccga ggacctcgga ggacgtgatc ggccgctccc
     5641 gggatcc
//
LOCUS       DASQQQ010000190         2978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_287728, whole genome shotgun sequence.
ACCESSION   DASQQQ010000190 DASQQQ010000000
VERSION     DASQQQ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287728"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VE646_07395"
     CDS             <1..190
                     /locus_tag="VE646_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019807692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79848.1"
                     /translation="NLLVADPDALGERAVRSGAVEVAPIQNQAYGLRQGRFTDPYGHV
                     WLIERPLAGEGGAWGRTS"
     gene            804..2117
                     /gene="istA"
                     /locus_tag="VE646_07400"
     CDS             804..2117
                     /gene="istA"
                     /locus_tag="VE646_07400"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZD79849.1"
                     /translation="MIDVHQWAEIRRLHFAEGLGIKAIVKRTGVARNTVRAAIRSQEP
                     PAYRRPARSSKLDPFKGEIVSLLKDDEDIPGKRVLEILQGLGYDGGKSILNEHLAQVR
                     PLFHKPRIYQRTLYLPGRIGQWDLWEPPFAIPVGYGQARRGYVLTGVLGYSRVGAGAL
                     VFSKSAPDLLWAMDRCLGQLGGVPRVAVFDREGALCADKTARHPHPTEPLARFAGHFG
                     FGVHFRPGADPEGKGVVERLHGYLETSFVPGRRFTSPEDFQTKLDAWFAEVANVRLHS
                     TIRCRPIDRLAEDRSATLPLPARSPDLTWRFHAPVHPDPYVRVDTNDYSVHPDAVGLV
                     VEVRVTHHDVTAVTKDGRVVAHHTRSLAPHRTITAAEHGRAIRQLREGTASRSEPLVE
                     IRDLGSYDRLLGLQAPELPGLSVQGAPENGSPGLPEPGLEAGTAR"
     gene            2114..2887
                     /gene="istB"
                     /locus_tag="VE646_07405"
     CDS             2114..2887
                     /gene="istB"
                     /locus_tag="VE646_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020663156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZD79850.1"
                     /translation="MSAKAELAHLFRALKAPAAAAALPELTKRARDGSWSYERFAEEL
                     LGAEHLARSAHGGEARIRQARFPGRKSLDEFDFSFQKSVSRQVLAHLSQLDFLAEAGN
                     VVFLGPPGTGKTHLSIALGIRACLAGHRVAFATATEWVARLADAHRQGRLEAELRRLQ
                     RIPLVVVDEIGYIPFDPEAANVMFMLVSSRYERASVIVTSNKPFSAWGEIFGDAVVAA
                     AMVDRLVHHAEVISLRGDSYRLKDRDLGRVVRPTAERVG"
BASE COUNT          455 a         1030 c         1010 g          483 t
ORIGIN      
        1 gaacctgctc gtggccgatc cagacgcgct cggcgagcgt gccgttcgaa gcggtgccgt
       61 ggaggtcgcg cccatccaaa atcaagccta tgggctccgc cagggacgct tcaccgaccc
      121 gtacgggcac gtctggctga tcgaacgccc attggccggg gaaggcgggg cgtggggtcg
      181 gacatcctga tccactcagc ccgcacggtg gtccccgctt ctcatgaggc ggtgagagat
      241 ctcggatcag cgatgctgga ggacctgaaa ggggtcggaa accaatcgtt ccgatcctgg
      301 cgccgggctc tggatcatcg ggcggtactc ccgtggtctg ctacttcatc ctgcggctga
      361 tcctgaggat cggctcacct gatgatgcga ccggggcgcg aggcggagat cctcgtcttc
      421 gccagcagct cgcggcctcg aagcgaacca gtcccggcct ggctgcggcg ccgggaccgg
      481 atgccgatcg cgggccctgt cgaggatgat gccgagggag cgctcctgcc tgcgccgcga
      541 gacgcccccc accggcaccg cccacgccca ccgcgcctgc gatccttact cccttctgcc
      601 atcgggtgtc agctcaccat ctcttgtccg gttccccggg gacccaagcg gcaccttgag
      661 gtcgcctcga tagccatgtc ggcgccggtt gaaaactgac ccccctgggc cggttgaatc
      721 ctgaccccct gggcttcctt ccgggactcg agggcccggg ggggtgagcc tggtcggcga
      781 tccgagccga ccagggggaa ggaatgatcg acgtgcacca gtgggcagag atcaggcgtc
      841 tgcactttgc cgaggggctg ggcatcaagg cgatcgtcaa acgcaccggg gtcgccagga
      901 acaccgtgcg ggcggcgatc cggtcacagg aaccacccgc gtaccgccgt cctgctcgtt
      961 cctcaaagct cgatcctttc aagggggaga tcgtgtcgct gctgaaggac gacgaggaca
     1021 tccccggcaa gcgcgtgctc gagatcttgc aggggctcgg gtacgacgga ggcaagtcga
     1081 tcctcaacga gcatctcgcc caggtccggc cgctgtttca caaaccccgg atctaccagc
     1141 gcacgctcta cctcccggga cggatcggcc agtgggacct gtgggagcca ccatttgcga
     1201 tccccgtggg ctacggccag gcccgccgcg gctacgtgct gaccggtgtc cttggctact
     1261 ccagggtggg cgccggtgcc ctggtgttct ccaagtccgc gcccgatctg ctctgggcga
     1321 tggatcggtg ccttggccag ctcggcgggg tgccccgcgt agccgtgttc gaccgggagg
     1381 gagcgctgtg cgcggacaag acggcccggc atccccatcc gaccgagccg ctcgctcgct
     1441 tcgccggcca cttcgggttc ggggtgcact ttcgccccgg agcagacccc gagggaaagg
     1501 gcgtggtgga gcggctgcac gggtacctgg agacttcgtt cgtccccggg cgacgcttca
     1561 cctcacccga ggatttccag accaagctcg atgcctggtt cgccgaggtc gccaacgtcc
     1621 ggctccacag cacgatccgg tgccgcccga tcgaccggtt ggcggaggat cgatcggcga
     1681 cgctgccgct ccccgcccgg tcgcccgatc tcacctggcg cttccacgcc cccgtccatc
     1741 ccgaccccta cgtccgggtg gacaccaacg actactcggt gcatccggat gcggtcgggc
     1801 tggtggtgga ggtcagggtc actcaccacg acgtcacggc cgtgaccaag gacggccggg
     1861 tggtggcgca ccacacccgt tccctcgccc cgcaccgcac gatcaccgcg gccgaacacg
     1921 gccgggccat ccggcagctg cgagaaggaa ccgccagcag gtccgaaccg ctcgtggaga
     1981 tccgcgacct cggctcctac gaccggctgt tgggcctgca ggccccagag ctgcccggtc
     2041 tgtcggtcca gggcgctccc gaaaacggct caccgggcct cccagagccc ggtttggaag
     2101 cggggacggc cagatgagcg ccaaggcgga acttgcccac ctgttccgag ccctgaaggc
     2161 cccggctgcc gcggccgcgc tgcccgagct caccaagcga gcccgtgacg gctcctggtc
     2221 ctacgagcgc ttcgccgagg agctgctcgg ggccgagcac ctggcccggt cggcgcacgg
     2281 aggcgaggcc aggatccgac aggcgcgctt tcccgggcgc aagtcccttg acgagttcga
     2341 cttcagcttc caaaagtctg tctcccgcca ggtgctggcc cacctttcgc agctcgactt
     2401 ccttgccgag gcaggcaacg tggtgttcct cggcccgccg ggcaccggca agacccacct
     2461 gtcgatcgcg cttgggatcc gggcctgcct tgccggacac cgggtggcct tcgccaccgc
     2521 cacggagtgg gtggctcgcc tggccgacgc ccaccggcag ggacggctgg aggccgagct
     2581 ccggaggctg cagcggatcc cgctggtggt ggtcgatgag atcggctaca tcccgttcga
     2641 ccccgaggcc gccaacgtga tgttcatgct ggtgtcctcg cgctacgagc gggcctcggt
     2701 gatcgtgacc tcgaacaagc cgttctccgc gtggggagag atcttcgggg acgccgtggt
     2761 ggccgcggcc atggtcgacc gcttggtgca ccacgccgag gtcatctcgc tgagaggtga
     2821 cagctaccgg ctgaaggacc gagacctggg aagggtggtg aggcctacgg ctgaacgtgt
     2881 cgggtagggg ggtcagcttt cggccggcct caaggggtca gttttcgagc ggcgttgaca
     2941 acgggatcga ggcgatccgc gtcgcaaccc tcatgggc
//
LOCUS       DASQQQ010000191         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_228944, whole genome shotgun sequence.
ACCESSION   DASQQQ010000191 DASQQQ010000000
VERSION     DASQQQ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228944"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VE646_07410"
     CDS             complement(<1..865)
                     /locus_tag="VE646_07410"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZD79851.1"
                     /translation="MSLTTLDGRYHVVRPLAAGGMGEVFLAHDAVLAREVAIKVLHRS
                     LAGDPGFIDRFRREARAAGALSHPNIVAVHDWGAVDGVYYMVMELVRGQSLRELLNAE
                     GLLAPAQTADVLLQVLGALGHAHRKGIVHRDVKPENVLVTPEGVAKVTDFGLARAYAD
                     ARATYAGSVTGTVQYLAPEQLQGEPADPRTDLYSLGIVAYELLTGSVPFGGETTLSIA
                     YKHVRERVPRPSLRNRAVPPGLDGWVASMTEKERELRPESAEEARRDLAQEAAGLAEA
                     APVGDLVVATPA"
     gene            995..1714
                     /locus_tag="VE646_07415"
     CDS             995..1714
                     /locus_tag="VE646_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79852.1"
                     /translation="MAEAPRQASEDEILQEGFARIEAAVEAGDTDLSKLGFWKLVRRV
                     KVDPLLSQRWADVVGRIDDKAFRAAVRPRFPIWFGNGVLALGTLAGGGALSVAVVAED
                     PTVSGGALVAAGVIWAVSLHDLAHWVTGRLAGMRFSSYFLGGMLPVPLYPGLKSDYAS
                     YLRADPRARVWMHASGALATKVAPFVPLTVWKLTAAPRWAALTLAGVGVLQIVTDILF
                     STRKSDWKKVRRERAIARARA"
     regulatory      1787..1899
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1888..>2282
                     /locus_tag="VE646_07420"
     CDS             1888..>2282
                     /locus_tag="VE646_07420"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HZD79853.1"
                     /translation="MDRRALALGVYVVTSGDLVKGRGHREVALAAIEGGATAVQLRAP
                     ELEDPSLLPLATELARACREAGVLLLVNDRLDVARQSGAHGVHLGQEDELEGARERLG
                     PDRVLGISVTSPPEALAAEGTGADYLGVTV"
BASE COUNT          301 a          775 c          880 g          326 t
ORIGIN      
        1 ccgccggcgt cgccacgacc aggtcgccga ccggagcagc ctcggccaat ccggccgcct
       61 cctgggcgag gtcgcgacgg gcctcctcgg cggactccgg tcgcagctcg cgctccttct
      121 cggtcatcga ggccacccag ccgtccaggc ccgggggcac ggcgcggttg cggagcgagg
      181 gcctcggcac gcgctcgcgc acgtgcttgt aggcgatgga gagcgtggtc tcgccgccga
      241 aggggacact gcccgtgagc agctcgtagg ccacgatgcc cagcgagtac aggtcggtgc
      301 ggggatcggc cggctcgccc tgcagctgct cgggcgccag gtactgcacg gttccggtga
      361 ccgagccggc gtacgtggcg cgggcatcgg cgtaggcccg agccaggccg aagtccgtga
      421 ccttggccac gccttcgggg gtcaccagga cgttctccgg cttcacatca cggtggacga
      481 tgccctttcg gtgggcgtgc ccgagggcgc cgaggacctg gagcagcacg tcggcggtct
      541 gggcgggggc gagcaggccc tcggcgttca ggagctcgcg caggctctgt ccccggacca
      601 actccatcac catgtagtag acgccgtcca ccgcccccca gtcgtggacg gccacgatgt
      661 tcggatggga cagggctccc gccgcccggg cctcgcgccg gaagcgatcg atgaacccgg
      721 gatcgccggc caggctccgg tgcaggacct tgatggcgac ctcccgggcc agcacggcgt
      781 cgtgggccag gaagacctcg cccatgcccc cggcggcgag cggccgcacg acgtggtacc
      841 gaccgtcgag ggtggttagg ctcatcccat ggtcgggtaa gcggacgacc gggtcgggtt
      901 cggtgcggct ggacgtgacg gaacctccgc tcgcgcgtgc tgtgaggggc cggggaagcg
      961 tccgggcaac atcctagcgt gcgtcggagg tgagatggcc gaagcaccac ggcaagcttc
     1021 cgaggacgag atccttcagg aggggttcgc ccggatcgag gcggcggtcg aagccggcga
     1081 caccgacctg tccaagctcg ggttctggaa gctggtgcgt cgggtcaagg tggatcccct
     1141 gctgtcgcag cggtgggccg acgtcgtcgg acggatcgac gacaaggcct tccgcgccgc
     1201 ggtgcgcccg cggttcccga tctggttcgg gaacggggtg ctggcgctcg gcaccctggc
     1261 cggcggcggc gccctgtcgg tggccgtggt ggcggaggac ccgaccgtgt cgggcggggc
     1321 gctggtggcg gcgggggtga tctgggcggt ctccctgcac gacctcgcgc actgggtgac
     1381 gggccggttg gccgggatgc ggttctcgag ctacttcctg ggcgggatgt tgcccgtccc
     1441 cctgtacccg gggttgaaga gcgactacgc gagctacctg cgcgccgacc ctcgggcccg
     1501 ggtctggatg cacgcttcag gcgcgctggc caccaaggtg gccccgttcg ttccgctgac
     1561 cgtttggaag ctcaccgccg ccccgcggtg ggcggccctg acgctcgccg gcgtgggcgt
     1621 gctgcagatc gtgaccgaca tcctgttctc caccaggaag agcgactgga agaaggtccg
     1681 acgcgagcgg gccatcgctc gcgcccgcgc ctgagggggg cgctcgacga gggcggaccc
     1741 cggccgccct cggctaaggt tcctggcgcc cgcgcctata cttgcgcctc gaagatcggg
     1801 gagctcgggc caccgggctg agaggccggc agaccgcgcc ggcgacccgc cgaacctgat
     1861 ccgggtaatg ccggcggagg gaggccgatg gatcgaagag cgctcgctct cggggtgtac
     1921 gtggtcacct cgggcgacct ggtgaagggc cgcgggcatc gggaggtggc gctcgccgcg
     1981 atcgagggtg gggccacagc cgtgcagctc cgggcccccg agctcgagga cccttccctg
     2041 ctgcccctcg ccaccgagct cgctcgagcc tgccgtgagg ccggggtgct cctcctggtg
     2101 aacgatcggc tggacgtcgc caggcagagc ggggcccacg gcgtccacct gggacaggag
     2161 gacgagctgg agggcgcgcg ggagcggctg gggcccgacc gggtcctcgg gatcagcgtg
     2221 acgtctccgc ccgaggccct tgcggcggag ggaaccggcg ccgactacct gggggtcacg
     2281 gt
//
LOCUS       DASQQQ010000192         3391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_313122, whole genome shotgun sequence.
ACCESSION   DASQQQ010000192 DASQQQ010000000
VERSION     DASQQQ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313122"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VE646_07425"
     CDS             complement(<1..192)
                     /locus_tag="VE646_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79854.1"
                     /translation="MAVTVLDLKRPALALERLEGIAGELIAELETQIPRVLHGSSATA
                     YVGRWRALAERLLTLVAREH"
     gene            complement(252..1751)
                     /locus_tag="VE646_07430"
     CDS             complement(252..1751)
                     /locus_tag="VE646_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZD79855.1"
                     /translation="MGDFGIGSWIGRRARIDPARTALVFEDRSWSYAELDRRIASLTA
                     ALIQLGVEPGDRVAYVGPNHPSFLETLFAVARAGAVLVPVNPGLDDEVAAFEMQDAGC
                     RLVIHAPGPPGIGLAEGERVSLDGSHGGRAYEHLIQGSVRPPERTVGLEDLAILPYTS
                     GTTGRPKGVMLTHANLTWNVVNMIGIADITGADVALATAPLFRMGGLGVTVLETLFMG
                     GTVVLTAGPDPDAVLDLIERHRITLVFAGPEILRGLPQHPRWERADLSSVRLCMTGGT
                     TVPEPLIRTYLERGLPLSQGYGLTEASPVVSVLSGDLLRTVGSAGPPVPLCDVRVVDG
                     DRAEVPAGSIGEILVRGPNVMQGYWHRPEATAHALIADGWLRTGDAGSLDEEGFLYVV
                     GRMADAFAWMGRTVHPYEIEKVLLEHPDVAECAAVGVPDQGTGQVAAAGVVLRAGSNA
                     DAGDLARFGREHLNERGLLGSVVLLDRIPRNPVGKVVRAELRERLTAAV"
     gene            complement(1766..2815)
                     /locus_tag="VE646_07435"
     CDS             complement(1766..2815)
                     /locus_tag="VE646_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZD79856.1"
                     /translation="MIVVGIDPHKQTHTAVAIDGATGRKVGELTVRARSRGFERLLSW
                     ARGLDEERGFAVEDGRHVSGALERHLIARGEGSVRVPPAMMFQARRADRVRGKSDPID
                     AEAVARAALAHPELPVATLAGIERDLDLLVAHRESLVNERTERISKLRWLLHDLDPDL
                     APPLRSLDRFVVLDRLAADLQAMEATTALKICLELIVRCREITARAKELEGEITSLAK
                     RHAGALLQIPGCGALTAAKIVAETAQVARFPTEGHYARYAGTAPLPVASGASHRHRFN
                     RRGNRQLNAAIHRIAVTQLRIHQPAIDYLARKRAEGMSKTEALRALKRCITRTVFKTM
                     TSMDQPAATTMAPAA"
     gene            3173..3328
                     /locus_tag="VE646_07440"
     CDS             3173..3328
                     /locus_tag="VE646_07440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZD79857.1"
                     /translation="MAEGRLVRETGGTGLGLSIVKHIVENHGGEIDVASELGRGTTVT
                     VTIPARA"
BASE COUNT          511 a         1220 c         1185 g          475 t
ORIGIN      
        1 atgctcgcgg gccaccagag tcaggaggcg ttcggcgagc gcccgccagc gccccacata
       61 ggcggtggcg gaggagccat ggaggacccg agggatctgg gtctcgagct cggcgatgag
      121 ctcgccggcg atcccctcca gccgttccag agccagggcg gggcgcttga gatcgagaac
      181 ggtgaccgcc atgatccgag cctaccgcaa gcgaaggcaa gcgcaagcgg aacgcaagct
      241 gactcggagc ttcagaccgc ggccgtcagg cgctcgcgga gctcggcgcg gaccaccttc
      301 cccaccgggt tgcgggggat ccggtccagc agtacgaccg acccgagcag gccccgctcg
      361 ttcaggtgct cgcgtccgaa ccgggccagg tctccagcgt cggcgttcga ccctgcgcgc
      421 aggaccacgc ccgcggcggc cacctgcccg gtcccctggt ctggcacgcc gacggcggcg
      481 cactcggcca cgtccgggtg ctccagcagg accttctcga tctcgtaggg gtggacggtc
      541 cgtcccatcc acgcgaaggc gtccgccatg cggcccacca cgtacaggaa gccctcctcg
      601 tccagcgatc ccgcgtcacc ggtccgcaac cagccgtcgg cgatgagcgc gtgggccgtg
      661 gcctccggac ggtgccagta gccctgcatc acgttcgggc cgcggaccag gatctcgccg
      721 atcgatccgg cggggacctc ggcgcggtcg ccgtccacca cccggacgtc gcacagcggc
      781 accggggggc cggccgagcc cacggtgcgg agcagatccc cgctcagcac gctcaccacg
      841 ggggaggcct ccgtgagccc gtacccctgg ctgagcggca gccctcgctc caggtaggtc
      901 cggatgagcg gctcggggac ggtcgtccct cctgtcatgc acagccgcac gctggacagg
      961 tcggcgcgct cccaccgagg gtgctggggc aaccctcgga ggatctccgg cccggcgaac
     1021 accagggtga tgcggtgtcg ttcgatcagg tccaggacag cgtccgggtc cgggccggcc
     1081 gtcaggacca cggtgccgcc catgaacagc gtctccagga cggtgacgcc gaggccgccc
     1141 atccggaaca gcggggccgt ggccagggcg acgtcggcgc cggtgatatc ggcgatcccg
     1201 atcatgttca ccacgttcca cgtcaggttg gcgtgggtca gcatcacgcc cttgggccgt
     1261 cccgtggtgc ccgaggtgta aggcaggatc gccaagtcct ccaaccccac cgttcgctcc
     1321 ggcggccgga ccgagccctg gatgaggtgc tcgtacgctc ggccgccgtg ggagccgtcc
     1381 agcgagaccc gctccccctc cgccaggccg atcccggggg gtccgggcgc atggatcacg
     1441 agccggcatc cggcgtcctg catctcgaag gccgccacct catcgtccag cccgggattc
     1501 accgggacca acaccgcgcc ggcccgggcc accgcgaaca gcgtctcgag gaaggacggg
     1561 tggttggggc cgacgtaggc cacgcggtcc ccaggctcga cgcccagttg gatcaacgcg
     1621 gcggtcaagg aggcgatgcg ccggtccagc tcagcgtagg accaggatcg gtcctcgaag
     1681 accagcgcgg tccgggccgg atcgatgcgg gcacggcgac cgatccacga gccgatgccg
     1741 aagtccccca tcggttgctc ctatgtcaag ccgccggcgc catcgtggtg gcggcgggct
     1801 gatccatcga agtcatcgtc ttgaacaccg tcctggtgat gcatcgcttc agggcacgca
     1861 gagcctcggt cttgctcatt ccttcggcac gcttcctggc caggtagtcg atcgcgggtt
     1921 ggtggatccg cagctgggtc acggcgatcc ggtggatcgc ggcgttcagc tggcggttgc
     1981 cgcgacggtt gaaccggtga cgatggctgg ctcctgaggc caccggcagc ggtgcggtcc
     2041 ccgcgtagcg ggcgtagtgg ccctcggtcg ggaaccgggc gacctgcgcg gtctcggcca
     2101 cgatcttggc agccgtgagc gccccgcagc cggggatctg cagcagggcc ccggcgtggc
     2161 gcttggcgag cgaggtgatc tcgccctcga gctccttggc gcgagcggtg atctcgcggc
     2221 accgaacgat cagctccagg cagatcttga gcgcggtggt cgcctccatc gcctgcaggt
     2281 ctgcggccag acgatccagc accacgaagc ggtccaggct gcgaagcggc ggggccaggt
     2341 cagggtccag gtcgtgcaga agccaccgga gcttggagat ccgctcggtt cgctcgttga
     2401 cgagcgattc ccggtgcgcc acaagcagat ccaggtcccg ctcgatccca gcgagggtgg
     2461 ccaccgggag ctcggggtgc gccagagccg cccgcgccac cgcctcggcg tcgatcggat
     2521 cggacttgcc ccggacccgg tcggcccggc gggcctggaa catcatcgcc ggcgggacac
     2581 gcacggagcc ctcgccgcgg gcgatcaggt gccgctccag ggccccggag acgtgacggc
     2641 cgtcctcgac ggcgaagccg cgttcctcat ccaggccccg ggcccagctg agcaggcgtt
     2701 cgaaccccct gctccgggct cggaccgtga gctcgccgac cttgcggccg gtcgccccgt
     2761 cgatcgcgac ggcggtgtgg gtctgtttgt gcgggtcgat ccccactacg atcactcctg
     2821 gtcccctttc ggacgcacac accggatggc aggaccaccg gcggacacgc ctcagtgggg
     2881 ctgagagcaa gctcctatca agtcacgccg gcaggccttc gccgacggcg ggcggcagaa
     2941 ccgatgcagg tcatcccgca ccgcgggaga cacgcaggct atgagccagc ccgccgtctg
     3001 gcggaggcta tcccgagacg gtctcgggat ctcgctgcgc gtgcatcctg acactgagtc
     3061 gtcaggctac cctccatcgc cggggccgcc ggtccacggg tcggagccgc gaccgtcgtt
     3121 cctgacgccc acaggcccta cgattccaac ttctcacgac ctaggaggga acatggcgga
     3181 aggcaggctc gtgcgggaga cgggcggcac gggtctgggt ctatccatcg tgaagcacat
     3241 cgtggagaac cacggtggcg agatcgacgt cgccagcgag ctcggccgcg gcaccaccgt
     3301 cacggtgacc atccccgccc gcgcctgagc gcgtggacga ccccaccgag ggcgctccgg
     3361 cgcccgcacc gcccggtgcc cggcgtccgg a
//
LOCUS       DASQQQ010000193         7008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_369490, whole genome shotgun sequence.
ACCESSION   DASQQQ010000193 DASQQQ010000000
VERSION     DASQQQ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7008
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369490"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..620)
                     /locus_tag="VE646_07445"
     CDS             complement(111..620)
                     /locus_tag="VE646_07445"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HZD79858.1"
                     /translation="MFEPCVLGVDPGVASTGVAAVTRAGGRIRVRWAETIRTPADQDE
                     PSRLWRLHRALAQAIAQHRPRELAVERVMWGRNQTSAMAVARATGVILLAAAQAGVPV
                     EEYLPAEVKVAVTGMGGAGKDRVRHALANAHRVEGVPPQVDAADAVAVAVCHLQQARL
                     RRAARAVAR"
     gene            complement(661..3216)
                     /locus_tag="VE646_07450"
     CDS             complement(661..3216)
                     /locus_tag="VE646_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HZD79859.1"
                     /translation="MAASRRGAGRLLVPAIVIVGMIAAATPGPGRADPGTGRSPTATG
                     RIAERALTSAADANEKGTPDSAEALEGAEQYARIRTLPAETVPAAAYRSARRQAAALA
                     STGGTWTEITRQPYQSDAAGYEDPVFSNSGGGAGFVSGRIQGLAVDGSTVFAAAADGG
                     VWRSRDRGRTWTAVGDHLPTLSGGSVAVNPADHSVWFGSGEASTAFENYAGLGVYRSR
                     NDGQTWRRVGGHELEGQMIGTLVFGRGFVFAATTGGVYRHRSRGDLSTHWQRVLAPGT
                     AGPYGFRFANDVAVRPRTNGRVVIAALGWRTGSVDYAGFWRSTKGGARGTWHRVRTRG
                     KLRSSQIGRASFAYSADGTRLYALVESWQYALSEPSALYGIFRSTSGDLRGPWERRAG
                     WQKLAGSGSAMAPFGNSYAPGVQAWYNQAIGVDPADRNHLYVGLEEVYESTNGGGTWT
                     TAGPYWNFTLPCSPSCLNTTHPDQHAVAFGPGVVYVGNDGGVYRRPRSRHTRGGWVNL
                     NRTLHTLQYYHAAVGQTARGDTYWGGLQDNGVGLLRPGASQQVSPFGGDGGDVIVHPS
                     KPKNAVVEYTDMDMALTTNGGASNGHTLAFREISPSCSAFTYTPSPCDPNPRFIAPFE
                     ADPESPDQHWVAGGQDVWETTEGWNTTCSESGCDWTIGHDLGSGNSTTALAVQGATVY
                     AGWCGGADRCNPGDFESGIDTNAGGSWQRVVDGSGNGGDPLPQRYITGLALEPGDPQH
                     VFVAYGGYSRRWIDGGGVGHVFESTDGGATWTGITGNLPDVPAADVEVVGDHLVLATD
                     LGVFTSALTSDDTWSRLGGGLPNAALNSLAIAPDGSFLVAVTHGRGLWKIAAP"
     gene            complement(3301..4053)
                     /locus_tag="VE646_07455"
     CDS             complement(3301..4053)
                     /locus_tag="VE646_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZD79860.1"
                     /translation="MSGHSKWSTIKRKKGAQDAKRSNLFAKLLRAVEVAAREGGGSVE
                     GNMTLASAVEKAKDASVPNENIERAIKRGTGELEGGARYEEVTYEGYGPGGVALFVET
                     LTDNRNRTAQDVRHAFSRSGGNLGETGSTAWMFTRKGVIQIEKDAAPDEVRLLEIAME
                     AGAEDLADAESTWEVTTDPTALIEVRDAIAGAGVPVLSAELSMLPQTQVPVEGGQARQ
                     VLALLEALEELDDVQNIYANFDIPEELMAEAG"
     gene            complement(4056..4613)
                     /gene="pdxT"
                     /locus_tag="VE646_07460"
     CDS             complement(4056..4613)
                     /gene="pdxT"
                     /locus_tag="VE646_07460"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HZD79861.1"
                     /translation="MKAGVLALQGDFREHARVLSDVGATPVEVRTPEELASVDALVIP
                     GGESTTIGKLARSAGLIEPIRERAAEGMPVLGTCAGMIVMASEVEGGDSPLGLVDIRV
                     RRNAYGRQVDSFEADVEVEGIDRPVRAVFIRAPVVERAGAGVRVLSSLEGRPVVLEQG
                     NLVVASFHPELVGEGGLHEYVLRKG"
     gene            complement(4616..5503)
                     /gene="pdxS"
                     /locus_tag="VE646_07465"
     CDS             complement(4616..5503)
                     /gene="pdxS"
                     /locus_tag="VE646_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HZD79862.1"
                     /translation="MERETGTFRVKSGLAEMLKGGVIMDVTNAEQAKIAEDAGACAVM
                     ALERVPADIRKEGGVARMADATKVEEIMSAVSVPVMAKARIGHFVEAQILQALGVDYV
                     DESEVLTPADEEHHIDKWAFTVPFVCGARNLGEALRRIAEGAAMIRTKGEAGTGNVVE
                     AVRHQRTILKETGWVQSLRPEQYVSAAKELGAPVELVRMVAEQGRLPVVNFAAGGIAT
                     PADAALMMQLGVDGVFVGSGIFKSSDPAPRARAIVEATTHFNAPEALAKVSRGLGEPM
                     PGLDIKELGEEGLLQVRGW"
     gene            complement(5545..6597)
                     /locus_tag="VE646_07470"
     CDS             complement(5545..6597)
                     /locus_tag="VE646_07470"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HZD79863.1"
                     /translation="MSLTRRGKAAIAGGVVVTVAVAAVGFFSLFPDKAPAFVRTAISG
                     VGLAPDPPPPDCPLTGRPAPHGAIPDRPALAVKVENLPEARPQSGLDRADVVYEEPVE
                     GGITRFIAIFQCSQADRVGPVRSGRTTDPKVLVQYGHPVIGYAGGAPPVVKAIDEAGL
                     IDVNFDVAASAYTRDPDRPAPHNLYTTTKALWKASRTKDGAPDPVFDHAPEIEGRSRR
                     AASVHLPFSSYSDVIWRWGKKQGAWQRFHGTEPHLLTDGRQVSAANVVVMQVPVSTGS
                     IIDAAGNASPEVSLTGSGRAWVFRDGRVIVGRWRRPALKDVTSFVTRDGSSIALAPGT
                     TWVELVPQSIGVQIAK"
     gene            complement(6689..>7008)
                     /locus_tag="VE646_07475"
     CDS             complement(6689..>7008)
                     /locus_tag="VE646_07475"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZD79864.1"
                     /translation="IFAFGFLLLILAPLFGQVIRFAVSRQREYLADAQGAMLTRYPPG
                     LASALRKIAGAPHAMRSANNATAHLWLDQPSRFPGERMGAMEKLFSTHPPIAERIRRL
                     QEM"
BASE COUNT          926 a         2632 c         2465 g          985 t
ORIGIN      
        1 gagtgcgccc accgtggagg tgggggcgtg cacctcgtac ccgacaccgt ggacgtcgat
       61 caccgcgcgg tcaacggcct tggcggccac cgcgccctgc aggaaggcga tcatcgcgcc
      121 accgcacggg ccgcccgccg caggcgcgcc tgttggagat ggcagaccgc cacggccacc
      181 gcgtcggcgg cgtcgacctg cgggggaacg ccttccacgc ggtgcgcgtt cgcgagggcg
      241 tgccgaacgc ggtccttgcc cgcgccaccc atcccggtga ccgccacctt gacctcggcc
      301 gggaggtact cctccaccgg cacgccggcc tgggccgcgg ccagcaggat cacgccggtg
      361 gcgcgagcca cggccatcgc gctggtctgg ttgcggcccc acatcacgcg ctccaccgcg
      421 agctcgcgcg ggcgatgctg ggcgatggcc tgggccaggg cccggtgcag gcgccacagc
      481 cgggacggct cgtcctggtc ggcgggggtc cggatggtct ccgcccaccg cacccggatg
      541 cgacccccgg cacgggtcac cgcggccacg cccgtggagg ccacgcccgg gtccacgccc
      601 agcacgcacg gctcgaacat gtgttcagtc tagggcccgg ggtggggccg ggcaacgaca
      661 ctacggcgcg gcgatcttcc acagccctcg cccgtgggtg acggccacca ggaagctccc
      721 gtccggcgct atcgccaggc tgttcagggc cgcgttcggc aggccgcccc ccagccgcga
      781 ccaggtgtcg tccgaggtca gggccgatgt gaagaccccc agatccgtgg ccagcacgag
      841 gtggtccccc accacctcga cgtcggcggc cggcacgtcg ggcaggttgc cggtgatgcc
      901 ggtccaggtg gcgccgccgt cggtggactc gaacacgtgg cccacccctc cgccgtcgat
      961 ccaccgacgc gagtagccgc cgtaggccac gaacacgtgc tgagggtcgc caggctcgag
     1021 ggccagcccg gtgatgtacc gctgcgggag cggatcgccc ccgttccccg agccgtccac
     1081 cacccgctgc cacgagccgc cggcgttggt gtcgatcccc gactcgaagt cgcccgggtt
     1141 gcaccggtcg gccccgccgc accagcccgc gtagaccgtg gcgccctgga ccgcgagcgc
     1201 ggtggtgctg ttgccgcttc ccaggtcgtg gccgatcgtc cagtcgcacc ccgactccga
     1261 gcacgtggtg ttccacccct cggtggtctc ccacacgtcc tgccccccgg ccacccagtg
     1321 ctggtcgggc gactcggggt cggcctcgaa gggcgcgatg aaccgggggt tcggatcgca
     1381 cggggacggc gtgtaggtga aggcggagca cgagggcgag atctcccgga aggccagggt
     1441 gtgcccgttc gaggccccgc cgttcgtggt gagcgccatg tccatgtccg tgtactcgac
     1501 caccgcgttc ttgggcttgc tcggatgaac gatcacgtcg ccgccgtcac cgccgaacgg
     1561 agagacctgc tgcgacgccc ccggcctcag caggccgacg ccgttgtcct gcaacccgcc
     1621 ccagtaggtg tcccccctcg ccgtctgccc cacggccgcg tggtagtact gcagcgtgtg
     1681 gagggtgcgg ttcaggttga cccatccccc gcgggtgtgg cgcgagcgcg gacgccggta
     1741 caccccgccg tcgttgccca cgtacaccac accgggcccg aaggccaccg cgtgctggtc
     1801 ggggtgcgtg gtgttcaggc agctcgggga gcagggcagc gtgaagttcc agtagggacc
     1861 ggccgtggtc caggtcccgc ccccgttggt gctctcgtag acctcctcca ggcccacgta
     1921 gaggtggttg cggtccgccg ggtccacccc gatcgcctgg ttgtaccagg cctgcacgcc
     1981 gggcgcgtag gagttgccga acggcgccat ggccgatccg gacccggcca gcttctgcca
     2041 gccggcccgg cgctcccagg gaccccggag gtcgccactg gtggagcgga agatgccgta
     2101 cagggccgag ggctccgaca gcgcgtactg ccacgactcc accagcgcgt acaggcgcgt
     2161 gccgtcggcc gagtaggcga agctggcccg tccgatctgc gaggagcgga gcttgccccg
     2221 ggtccgtacc cggtgccagg tgcccctcgc gccgcccttg gtcgaccgcc agaaccccgc
     2281 gtagtcgacc gacccggtcc gccagcccag cgccgcgatc accacgcggc cgttggtccg
     2341 ggggcgcacg gccacgtcgt tcgcgaagcg gaagccgtac gggccggccg tcccgggggc
     2401 gagcaccctc tgccagtgcg tcgacaggtc gccccgggac cggtgtcggt acacgccgcc
     2461 ggtggtggcc gcgaacacga agccccggcc gaagaccagc gtcccgatca tctggccctc
     2521 gagctcgtgc ccgcccaccc ggcgccaggt ctggccgtcg ttgcgggagc ggtagacgcc
     2581 gaggcccgcg tagttctcga aggcggtgct ggcctccccg ctcccgaacc agacggagtg
     2641 atcggccggg ttcacggcga ccgagccgcc cgagagcgtg ggcaggtggt cgccgacggc
     2701 ggtccaggtg cgcccccggt cccgcgagcg ccacacgccg ccgtccgcgg cggccgcgaa
     2761 gaccgtggag ccgtccacgg ccaggccctg gatgcgcccc gagacgaagc ccgcgccgcc
     2821 gcccgagttc gagaagaccg ggtcttcgta cccggcggcg tcggactggt agggctggcg
     2881 ggtgatctcg gtccaggtcc cgccggtcga ggcgagggcg gcggcctgcc gccgggcgga
     2941 gcggtaggcg gcggccggga ccgtctcggc cggcagggtg cggatccggg cgtactgttc
     3001 ggccccctcc agggcctcgg cgctgtcggg cgtgcccttc tcgttcgcgt cggcggccga
     3061 ggtgagcgcc cgctcggcga tgcggccggt ggcggtgggc gaccggccgg ttcccggatc
     3121 ggcccgaccg gggccgggag tggcggcggc gatcatcccg acgatcacga tcgccgggac
     3181 caggagccgt ccggcacctc gacgtgatgc tgccatggcc gctccccctc gctgctcgcg
     3241 gaccgaggaa cggtagggca tcggggggga agcttcaacg gaaccgctcg cgggccgggc
     3301 tcagccggcc tcggccatca gctcctcggg gatgtcgaag ttcgcgtaga tgttctggac
     3361 gtcgtccagc tcctccaggg cctccagcag ggccaggacc tgccgggcct gcccgccctc
     3421 cacggggacc tgcgtctggg gcagcatcga cagctcggcg gacagcaccg gcacgcccgc
     3481 gccggcgatc gcgtcccgca cctcgatcag cgcggtgggg tcggtggtga cctcccaggt
     3541 gctctcggcg tccgccaggt cctcggcccc ggcctccatc gcgatctcca ggaggcggac
     3601 ctcatcgggg gcggcgtcct tctcgatctg gatcacccct ttgcgcgtga acatccaggc
     3661 ggtgctgccc gtctcaccga ggttcccgcc gctgcggctg aaggcgtggc gcacgtcctg
     3721 cgcggtgcgg ttccggttgt cggtcagggt ctccacgaac agggccacgc cgcccggacc
     3781 gtagccctcg taggtgacct cctcgtacct cgccccgccc tccagctcgc cggtgccacg
     3841 cttgatggct cgctcgatgt tctcgttggg gaccgaggcg tccttggcct tctccacggc
     3901 cgaggccagc gtcatgttgc cctcgacgct gccgccgccc tcgcgggccg cgacctccac
     3961 cgcgcgtagg agcttcgcga acaggttgct gcgcttggcg tcctgggcgc ccttcttgcg
     4021 cttgatcgtg ctccacttgg aatggcccga catcgctacc ccttgcgcag gacgtactcg
     4081 tggagcccgc cctcgcccac gagctccgga tggaaggatg ccacgaccag gttcccctgc
     4141 tccagcacga ccggtcgccc ctcgaggctc gagagcaccc ggacccccgc cccggcgcgc
     4201 tccacgacgg gcgcgcggat gaacaccgct cggaccgggc ggtcgatgcc ctccacctcc
     4261 acgtcggcct cgaacgagtc cacctgccga ccgtaggcgt tgcgacggac ccggatgtcc
     4321 accaggccga gcggcgagtc gcccccctcg acctcggagg ccatcacgat catgccggcg
     4381 cacgtcccga gcaccggcat cccctcggcc gcccgctcgc ggatcggctc gatcaggccg
     4441 gccgaacgcg ccagcttgcc gatggtcgtg gactcgccgc cggggatcac cagcgcgtcg
     4501 accgacgcga gctcctcggg ggtgcggacc tcgacgggcg tggcgccgac gtccgacagc
     4561 actcgcgcgt gctcgcggaa gtcaccctgc agcgcgagga cgccggcctt caccgctacc
     4621 agccgcggac ctgcagcagg ccttcctcgc ccagctcctt gatgtccagg cccggcatgg
     4681 gctcccccag gccccgggac accttggcca gggcctcggg ggcgttgaag tgcgtggtgg
     4741 cctccacgat ggcccgggcc cgcggcgccg gatcggagga cttgaagatc cccgagccca
     4801 cgaacacccc gtccaccccg agctgcatca tgagcgcggc gtccgcgggc gtggcgatgc
     4861 cgccggcggc gaagttcacc acgggcagcc ggccctgctc ggcgaccatg cgcaccagct
     4921 ccaccggggc ccccagctcc ttggccgccg agacgtactg ctcgggacgc aggctctgga
     4981 cccagccggt ctccttgagg atcgtccgct ggtgccgcac ggcctccacc acgttgcccg
     5041 tgccggcctc ccccttggtc cggatcatcg ccgccccctc ggcgatccgc cggagcgcct
     5101 cgcccaggtt ccgggcgccg cacacgaagg ggacggtgaa ggcccacttg tcgatgtggt
     5161 gctcctcgtc cgcgggggtc aggacctcgc tctcgtccac gtagtccacc ccgagcgcct
     5221 ggaggatctg agcctcgacg aagtgaccga tcctggcctt ggccatgacc gggacggaga
     5281 cggcactcat gatctcctcg accttggtgg cgtcggccat gcgagccacg ccgccctcct
     5341 tgcggatgtc ggccggcacg cgctcgagcg ccatgacggc gcaggcgccg gcgtcctcgg
     5401 cgatcttggc ctgctcggcg ttcgtgacgt ccatgatcac gccgcccttg agcatctcgg
     5461 ccaggcctga tttcacgcgg aaggtgccgg tctcacgttc cacgggtctc cctcctcgaa
     5521 ggcgtaccgc cagtgtaccg cccgctactt ggcgatctgg accccaatcg actgggggac
     5581 cagctccacc caggtggtgc ccggcgcgag cgcgatgctc gagccgtcgc gggtcacgaa
     5641 gctggtgacg tccttcaggg cggggcggcg ccaccgcccc acgatcaccc ggccgtcgcg
     5701 gaagacccag gcccggcccg agccggtcag ggagacctcg ggcgaggcgt tgccggcggc
     5761 gtcgatgatg ctgcccgtgg ataccgggac ctgcatcacc accacgttgg cggccgagac
     5821 ctggcggccg tcggtcagca ggtgcggctc cgtgccgtgg aagcgctgcc aggccccctg
     5881 cttcttgccc catcgccaga tcacgtccga gtaggacgag aagggcaggt gcacgctcgc
     5941 ggcccgcctg gagcgcccct cgatctccgg cgcgtggtcg aacacggggt ccggagcgcc
     6001 atccttcgtc cggctcgcct tccagagcgc cttggtggtc gtgtacaggt tgtgcggcgc
     6061 gggccgatcc gggtcgcgcg tgtaggccga ggcggccacg tcgaagttca cgtcgatcag
     6121 gccggcctcg tcgatcgcct tcaccaccgg cggcgctccg cccgcgtacc cgatcaccgg
     6181 atgcccgtac tggaccagca ccttcgggtc ggtggtgcgc cccgaacgca ccggccccac
     6241 ccggtcggcc tgggagcact ggaagatcgc gatgaaacgg gtgatcccgc cctccacggg
     6301 ctcctcgtag accacgtcgg cccggtccag gcccgactgg ggacgggcct cgggcaggtt
     6361 ctccaccttg accgcgagcg ccggccggtc cgggatcgct ccgtgcgggg ccgggcggcc
     6421 ggtcagcgga cagtcgggcg gcggcgggtc gggggccagg cccaccccgg atatggcggt
     6481 gcggacgaac gccggcgcct tgtccgggaa cagggagaag aagccgaccg ccgcaacggc
     6541 gacggtcacg accacaccgc cggcgatggc ggccttgcct cggcgcgtga gggacacgga
     6601 acgacctcct cgatcgtggg ggcgcgcgat cggccgttcc cggccgggcg ctcggatgct
     6661 acctgcccga gcgccgggcc tcaacgacct acatctcctg cagtcgccgg atccgctcgg
     6721 cgatcggagg atgggtggag aacagcttct ccatcgcccc catcctctcc ccagggaacc
     6781 gggagggctg atcgagccag agatgggcgg tggcgttgtt cgccgagcgc atcgcgtggg
     6841 gggcgccggc gatcttccgg agcgccgagg ccagccccgg cgggtagcgc gtcagcatcg
     6901 cgccctgggc gtcggccagg tactcgcgct ggcgcgagac cgcgaagcgg atgacctgcc
     6961 cgaacaacgg cgccaggatc agcaggagga agccgaacgc gaagatca
//
LOCUS       DASQQQ010000194         9384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_71331, whole genome shotgun sequence.
ACCESSION   DASQQQ010000194 DASQQQ010000000
VERSION     DASQQQ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71331"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VE646_07480"
     CDS             complement(<1..336)
                     /locus_tag="VE646_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZD79865.1"
                     /translation="MNDRVRRAEAILRAACGERLRTRFPLAPLTTFRIGGPAALYLEP
                     GSETDLVAAGEALRTTSLPFVVLGKGSNLLVSDAGFPGLVLRLGRGYRWVGRDGERLD
                     VGASMPLPAL"
     gene            complement(333..1754)
                     /gene="murC"
                     /locus_tag="VE646_07485"
     CDS             complement(333..1754)
                     /gene="murC"
                     /locus_tag="VE646_07485"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015749042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HZD79866.1"
                     /translation="MSWAYAPPPGSVPTLEVPDLGTVSSVHMIGVGGAGMRNLAKLLL
                     ARGIAVSGSDLKDSKGLRNLEAIGVRVSVGHRAEQVGEPDAVIVSSAILERNVELAEA
                     RRRGIRVWVRAQALAAAARGKRAIAVSGTHGKTTTTSMVGAILERVGLDPTYVVGGEP
                     NESGSGARSGAGELFVAEADESDGSFLLFRPEVGIVTNVEVDHVDFYPGGLTEIRAAF
                     GAFAQRSEHIVVCADDPGAMEATARHAARVTYGLAEGAAVRVEPTSPGPGGARGVVHA
                     AGGRAPLELAVDGEHNLRNAAAAVAAAGLVGVSVEAAVEGLRGFRGVHRRFEHRGSAR
                     GATFYDDYGHVPTELAVTLDVARRTDPARLVAVFQPHRYVRTQALWRELGASLVEADL
                     IVVTDVYAADQDPIPGVTGKMVVHGVTLATEGKRVVYLPRRGDVVSFLEAEVRPGDLV
                     ITMGCGDVWMLGDAAMERIAEAG"
     gene            complement(1751..2851)
                     /gene="murG"
                     /locus_tag="VE646_07490"
     CDS             complement(1751..2851)
                     /gene="murG"
                     /locus_tag="VE646_07490"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HZD79867.1"
                     /translation="MKVTIAAGGTAGHIFPALALGRRLADEGVQVGFVGTAAGQESRL
                     VPAAGFAFAAVEARPFVRTVSIEMVRAPVAALRSVRQCRPLVEDSDVVVGMGGYASVP
                     AGLAALRGRRPLVLHEQNAVPGLANRSLAIPARSVALAFAEAARLLPKRTRTVVTGNP
                     VRAEILAVPEDRETLAKEARSELGLDPDRATVVVFGGSQGALHVNRATVEAVERLRHR
                     GDLQILLLTGPANLAEVRRRLSENSIPLIRTVPFLERMELAYAVADLIVGRAGATTVA
                     EISVCGLPSILVPYPYATARHQEANARALAHAGAAAILMDHALSGAVLASRITELIGD
                     RGRLRVMGARASAWARPDAAEALARVVREAAR"
     gene            complement(2848..4083)
                     /gene="ftsW"
                     /locus_tag="VE646_07495"
     CDS             complement(2848..4083)
                     /gene="ftsW"
                     /locus_tag="VE646_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HZD79868.1"
                     /translation="MARVERAMTAPRRLRLVASAADPVRARGADTRTQAATRRTLLAL
                     IGSTAFLVLGGLVMVLSASSVSAFAQYGSSFLFFKRQVAYAVVGSGALLLASRMRYQA
                     WKRLSAPLLVIAMVLLAMVLLHPTAGVSAYGATRWIQLGPVTIQPSEIAKLGVVVFAA
                     AILARKGRRLDELGHLALPLLPVIGIVCGLVFVQPDLGTTTILVGTTLLLLFVAGVRL
                     RYLLAGTAAMVATAMVALKPYQWARITAFLHPEADPRNTGYQLLQSLIALGSGGWFGV
                     GLGASRQKWMYVPNAHTDFIFSILGEELGLIGELVVLAAFATLIYAGIRIAVRAPDAF
                     GRLLAAGIVSWFGLQAVVNLGAVTGLLPITGVPLPLLSYGGSSLVVSLAAVGILVNIA
                     RSPERSGRRSGTPPRGLAA"
     gene            complement(4086..5417)
                     /gene="murD"
                     /locus_tag="VE646_07500"
     CDS             complement(4086..5417)
                     /gene="murD"
                     /locus_tag="VE646_07500"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HZD79869.1"
                     /translation="MTGRFAHERVVVVGLGVAGRAAARVLAEEGARVRVSEARPDPGG
                     VDEIASLGVEVRVGGHHPEHLDGASAVVASPGVSEHAPVLGWARERGVPIWSELDVGA
                     RLCEVPYVAITGTNGKSTTTKLVATMMQAAGLDAVACGNIGHPFSIAAREGHEALAVE
                     ASSFQLRFHHWLHPRVSVLLNVAPDHIDWHGSIAGYAASKARIFALQGDGDVHVGNAE
                     DPAAAQISRRASCEQIWFRLGEPGEGEVGYADGELRSRFHEAVDLGRPALDTAGFRAD
                     AAAAAAAGLAFGLDGEAVGTGLSRMEPLPHRGEAVAHVGGVVFLDDSKATNVHAALNA
                     LAGKGDVVLIAGGLSKGAELGPLVTAAPSLAGLVAIGEAASELVSLFEGLVAVRKAGS
                     IEEAVRTAFELCPRPGTVLLAPACASWDMFRDYTERGERFARAALALGEEV"
     gene            complement(5414..6463)
                     /gene="mraY"
                     /locus_tag="VE646_07505"
     CDS             complement(5414..6463)
                     /gene="mraY"
                     /locus_tag="VE646_07505"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HZD79870.1"
                     /translation="MRAILLAAAVGLAVTLLGTPVAIRAFRVWGWGQHIREDGPRTHM
                     EKMGTPTMGGTVIVVGIIAAYVVTRVVFATVTAAGIAVLVATVGLALVGFLDDFIKVR
                     HRRSLGLSKTGKIAGQALVAVIFALIAVKAAHVSTDLSFVRPTGLGLGLGFYVWTMVI
                     LGGASNAVNLTDGLDGLASGSSILVLSAYVYISFWQYRHTCGAVFDPACYRVGVNAAL
                     DTAIVAGGAVGAVAGFLWWNAAPARIFMGDTGSLALGGLIGALAMVTETQLLLVILGG
                     LFVLEATSVILQIIAFRGFGRRIFRMSPIHHHFELAGWPEFTVIVRFWIIAGMCVAIG
                     IGLFYADFLARGGTG"
     gene            complement(6460..7821)
                     /gene="murF"
                     /locus_tag="VE646_07510"
     CDS             complement(6460..7821)
                     /gene="murF"
                     /locus_tag="VE646_07510"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HZD79871.1"
                     /translation="MRPRRLSEVARATDGRLRGSDATVSSVGIDSRRVGAGDLFVALP
                     GEHTDGHAFVADALHRGAAGAVVGRTVSADGPTIEVRDTGQALLALAADERASMDVTV
                     IGVTGSNGKTSTKDLAAAVLASRFRTHASPASFNNEIGLPLTVLGAPRGTEVLVAEMG
                     ARRAGDVALLSRIARPSLVVVTNVGVAHMEVFGSWGAITEAAAEPVEALDARGTALLN
                     ADDPLVLALAERTPARVITFGTGDHAEVRADDLVLNRSGTVSFVLVVGEDREPVELAV
                     PGAHMVSNALAATACGIALGVSPAECAASLKGARISRWRMERFTTEQGVSVVNDAYNA
                     NPESMAAGLRTARWMASEGRLVAVLGHMAELGPIAVEEHERLGELVVRLGVDRLITVG
                     EQARSIARSAVREGQLPEDVASYEDPDQVVEDLRGWLRAGDVVLIKGSRVAGLERLAE
                     ALR"
     gene            complement(7818..9284)
                     /locus_tag="VE646_07515"
     CDS             complement(7818..9284)
                     /locus_tag="VE646_07515"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HZD79872.1"
                     /translation="MAPHTPEARPLSELLTSLAGPELRGDPTTTVTSLAYRSQEVEPG
                     SLFFCIPGSRADGHDFAAEAVALGASALVVERGLDLSVPQVLVPSVRGAMGPISAAFF
                     ARPSDRLTMVGVTGTNGKTTTTYLLESVFRASGLVPGVVGTTGVRIDGEPVPFPRTTP
                     EAPDLHRLLRRMVDRGVEAVAMEVSSHGLDQHRVDAVRYRCAVFTNLTQDHLDYHASL
                     EDYFQAKARLFTPELSDRAVVNHDSPEGRRLLDGVLPTLSYGIEPGADVRATEVRATS
                     EGIAFSVDGRSIRSSLRGRFNVENCLAALAASRSLGIDDAAAVIGIERVRGVPGRVET
                     VEEGQGFLVMVDYAHTPDSVENVLRAARQLATGRLIVVFGCGGDRDRGKRPLMGKVAT
                     SLADLTVVTSDNPRSEEPAAILADIEPGARAGGGPYLVEADRRTAIRVAVERAQPGDV
                     VVIAGKGHETYQELADRTIPFDDRLVVAEELRGRMGTR"
BASE COUNT         1454 a         3629 c         3135 g         1166 t
ORIGIN      
        1 cagggcgggg agcggcatcg aggccccgac gtccaggcgc tccccgtccc gccccaccca
       61 ccggtacccg cgccccaggc gcagcaccaa cccggggaac cccgcgtcgg acaccagcag
      121 gttggatccc ttgcccagca ccacgaaggg gagcgaggtc gtccgcaggg cctcccccgc
      181 ggccaccaga tcggtctcgg acccgggctc gaggtacagg gcggccggcc cgccgatgcg
      241 gaacgtggtg aggggggcca gggggaagcg cgtccggagc cgctctccgc aggccgcccg
      301 gaggatcgct tcggctcgtc ggacccggtc gttcatccgg cctccgcgat ccgctccatg
      361 gccgcgtccc ccagcatcca cacgtccccg catcccatcg tgatcaccag gtcgccgggt
      421 cggacctcgg cctccaggaa gctcaccacg tcgcctcgac gggggaggta gacgacgcgc
      481 ttgccctcgg tcgccagggt cacgccgtgg accaccatct tcccggtgac ccccgggatg
      541 gggtcctgat cggccgcgta cacatcggtc accacgatca ggtccgcctc caccaggctg
      601 gcgccgagct cccgccacag ggcctgggtc cggacgtagc ggtggggctg gaagacggcg
      661 accagccggg cggggtcggt cctgcgagcc acgtccaggg tcaccgcaag ctcggtgggc
      721 acgtgcccgt agtcgtcgta gaacgtggct ccccgggccg agcctcggtg ctcgaaccgg
      781 cggtgcaccc cgcggaagcc ccgcagaccc tccaccgccg cctcgacgga gacccccacc
      841 agccccgcgg cggccacggc cgcggccgcg ttgcgcaggt tgtgctcccc gtccaccgcc
      901 agctccagag gggcgcgacc gcccgccgcg tggacgacgc cccgagcccc accgggtccg
      961 ggcgacgtcg gctccacccg taccgccgct ccctcggcga ggccgtacgt gacgcgggcg
     1021 gcgtgacggg ccgtcgcctc catcgcgccc ggatcgtccg cacagaccac gatgtgctcg
     1081 ctccgctgcg cgaacgctcc gaacgccgcc cggatctcgg tgaggccacc cggatagaag
     1141 tccacgtggt cgacctcgac gttggtcacg atcccgacct cgggccggaa gaggaggaag
     1201 gagccgtcgc tctcgtcggc ctcggccacg aacagctcgc ccgcgccgga gcgagccccg
     1261 ctcccgctct cgttcggctc accccccacc acgtacgtgg gatcgagccc gacccgctcc
     1321 aggatggccc ccaccatcga cgtggtggtg gtcttgccgt gggtcccgga gacggcgatc
     1381 gcgcgcttgc cgcgggccgc ggcggccagg gcctgggcgc gcacccagac ccggatccca
     1441 cgtcgccggg cctcggccag ctccacgttg cgctccagga tcgcgctcga cacgatcacg
     1501 gcatcgggct ctcccacctg ctcggctcgg tgacccaccg acacacgcac cccgatcgcc
     1561 tcgaggttcc gcaggccttt cgagtccttg aggtcggacc cgctcaccgc gatgccccga
     1621 gcgagcagca gcttggcgag gttccgcatg ccggctcctc ccaccccgat catgtgcacg
     1681 gagctcacgg tgcccagatc ggggacctcc agggtcggga cgctccccgg tggcggcgcg
     1741 tacgcccagc tcatcgggcg gcctcccgca ccacgcgggc cagggcctcc gcggcgtccg
     1801 gacgggccca ggccgaggcc cgcgcgccca tcacccgcaa ccgacctcgg tcaccgatca
     1861 gttcggtgat ccgcgaagcc aagaccgctc ccgacagggc gtgatccatc aggatcgctg
     1921 cggctccggc atgcgccagg gcgcgggcgt tcgcctcttg atgcctcgcg gtcgcgtacg
     1981 ggtagggcac gaggatcgag ggcaggccac acaccgagat ctcggccacc gtggtcgcgc
     2041 ccgctcgccc gacgatcagg tccgccacgg cgtacgcgag ctccatccgt tccaggaacg
     2101 ggacggtgcg gatgagcggg atcgagttct cggacaggcg ccgccggacc tcggccaggt
     2161 tggccggacc ggtcagcagg aggatctgca gatccccgcg atggcgcagc cgctccaccg
     2221 cctccaccgt ggctcggttc acgtgcaggg ccccctggct ccccccgaag accaccaccg
     2281 tggctcggtc gggatccaac cccaactcgc tccgagcctc cttggccaag gtctcccggt
     2341 cctcgggcac ggccaggatc tcggctcgca cggggttgcc ggtgacgacc gtccgggtcc
     2401 gcttgggcag gagccgggcg gcctcggcga aggccagcgc gaccgagcgc gcggggatcg
     2461 ccagggaacg gttggccagg ccggggacgg cgttctgctc gtgcaggacc aacgggcgcc
     2521 ggccgcggag cgcggccagc ccggcgggga cgctcgcgta cccgcccatc cccaccacca
     2581 cgtccgagtc ctccaccagc ggccgacact ggcggaccga tcggagcgcc gccaccggcg
     2641 cccgcaccat ctcgatggac accgtgcgga cgaacggtct cgcctccacc gccgcgaagg
     2701 cgaagccggc cgcgggaacc aggcgcgact cctgccctgc cgccgtgccg acgaagccga
     2761 cctgcacacc ctcgtccgcc aaccgtcgcc ccagggcgag ggccgggaag atgtgtccgg
     2821 ccgtcccccc ggccgcgatc gtcaccttca tgcggcgagc cccctcggag gggtcccgct
     2881 ccgccgcccg gaccgctccg gcgagcgggc gatgttcacc aggatcccca ccgcggcgag
     2941 cgagaccacc agcgaggacc ccccgtagga cagcaggggc agcggcacgc cggtgatcgg
     3001 cagcagcccg gtcaccgccc ccaggttcac caccgcctgc agaccgaacc aggagacgat
     3061 ccccgccgcc aggagccgtc cgaaggcatc gggcgctcga accgcgatgc ggatgcccgc
     3121 gtagatcagc gtggcgaacg ccgccaggac caccagctcg ccgatcagcc ccagctcctc
     3181 gccgaggatg gagaagatga agtcggtgtg cgcgttcggg acgtacatcc acttctgacg
     3241 gctcgcgccg aggcccaccc cgaaccaccc ccccgatccg agcgcgatca gcgactggag
     3301 caactggtag ccggtgttgc gtggatcggc ctccgggtgc aggaaggcgg tgatcctcgc
     3361 ccactggtag ggcttcaggg ccaccatggc cgtcgcaacc atcgcggccg tgccggcgag
     3421 caggtagcgg aggcgaaccc cggccacgaa cagcagcagc agcgtcgtac ccaccaggat
     3481 cgtggtcgtc ccgaggtccg gctggacgaa caccagtccg cagacgatgc cgatcaccgg
     3541 cagaagcgga agggccaggt ggcccagctc gtccagccgc ctccccttcc gagccaggat
     3601 ggcggccgcg aacaccacga cgcccagctt ggcgatctcc gagggctgga tagtcaccgg
     3661 accaagctgg atccacctgg tcgcgccgta cgcggacacg ccggcggtgg gatggagcag
     3721 caccatcgcg agcagcacca tcgcgatcac cagcagcgga gcggacaggc gcttccacgc
     3781 ctggtaccgc atcctcgagg cgaggagcag cgcccccgat cccaccaccg cgtaggccac
     3841 ctgccgcttg aagaacagga agctcgatcc gtactgggcg aaggcggaca ccgagctcgc
     3901 cgacaggacc atcaccaacc ccccgagcac caggaaggcg gtcgatccga tcaacgccag
     3961 cagcgttcgg cgggtcgcgg cctgggtgcg ggtgtcggca cctcgggccc gtaccggatc
     4021 ggccgccgat gccaccagcc ggagtcggcg gggggcggtc atcgctcgct cgacgcgggc
     4081 cacgatcaga cctcctcccc caacgccagc gcggcgcggg cgaaccgctc cccccgctcg
     4141 gtgtagtcgc ggaacatgtc ccagctcgcg caggccgggg ccagcagcac ggtgcctggc
     4201 ctcgggcaca gctcgaacgc cgtgcggacc gcttcctcga tcgagccggc cttgcgcacc
     4261 gccaccaggc cctcgaacag cgagacgagc tcggatgccg cctcgccgat cgccaccaga
     4321 ccggccaggg acggagcggc cgtgaccagg ggacccagct cggcaccctt ggagagcccg
     4381 ccggcgatca ggaccacgtc ccccttgccg gccagggcgt tcagcgcggc atgcacgttc
     4441 gtggccttcg agtcgtcgag gaagaccacg ccccccacgt gcgccaccgc ctcgccccga
     4501 tgcggcagcg gctccatccg cgacagcccg gtgccgaccg cctccccgtc caggccgaag
     4561 gccaacccgg cggccgcggc ggcggcggcg tcggcccgga accccgccgt gtccagggcc
     4621 gggcgtccca gatcgaccgc ttcgtggaac cgggagcgga gctcaccgtc cgcgtagccg
     4681 acctcaccct cgcccggctc gccgagccgg aaccagatct gctcgcacga ggctctccga
     4741 gagatctggg cggcggcagg gtcctccgcg ttgcccacgt ggacgtcacc gtcgccttgc
     4801 agggcgaaga tccgggcctt ggaggccgcg taccccgcta tcgagccgtg ccagtcgatg
     4861 tggtcgggcg ccacgttcag cagcaccgac acccgcgggt ggagccagtg atggaagcgc
     4921 agctggaacg acgaggcctc caccgcgagc gcctcgtgcc cctcccgcgc ggcgatcgag
     4981 aacgggtggc cgatgttgcc gcaagccacc gcgtcgagcc cggccgcctg catcatggtg
     5041 gccacgagct tggtggtggt cgacttgccg ttcgtgcccg tgatcgccac gtacggcacc
     5101 tcgcacagcc gggcgcccac gtccagctcg ctccagatgg ggacaccgcg ctcccgcgcc
     5161 catccgagga ccggagcgtg ctcggagacc ccgggactgg ccaccaccgc cgatgccccg
     5221 tccaggtgct cggggtggtg gccgcccacg cggacctcga ccccgagcga cgcgatctcg
     5281 tccacgcccc ccggatcggg ccgcgcctcg ctcacccgaa cccgggctcc ctcctcggcc
     5341 aggacccggg ccgcggcccg gccggccacc ccgagaccca cgaccaccac gcgctcatgg
     5401 gcgaaccggc cggtcatccc gtgccccccc tggccaggaa gtccgcgtag aacaggccga
     5461 tgccgatcgc cacgcacatc ccggcgatga tccagaaccg aacgatcacc gtgaactccg
     5521 gccagccggc gagctcgaaa tggtgatgga tgggagacat ccggaagatc cggcggccga
     5581 accctcggaa ggcgatgatc tgcaggatca ccgaggtcgc ctcgaggacg aacaggccgc
     5641 ccaggatcac cagcagcagc tgcgtttcgg tcaccatggc gagcgcgccg atcagccctc
     5701 ccagggccag ggaccccgtg tcgcccatga agatccgggc cggggcggcg ttccaccaca
     5761 ggaacccggc caccgccccc accgctcccc cagccacgat ggccgtgtcg agcgcggcgt
     5821 tcacaccgac cctgtagcag gccgggtcga agaccgcgcc gcacgtgtgc cggtactgcc
     5881 agaaggagat gtagacgtac gccgacagca cgaggatcga ggagccggag gccaatccgt
     5941 ccagaccgtc ggtgaggttc accgcgttcg aggctccccc caggatcacc atggtccaca
     6001 cgtagaagcc caggcccaga ccgagtccgg tcggacggac gaaggacaga tcggtggaga
     6061 cgtgggcggc cttcacggcg atcagggcga agatcaccgc caccagcgcc tgcccggcga
     6121 tcttcccggt cttggacagc cccagggaac ggcggtgccg gaccttgatg aagtcgtcca
     6181 ggaaccccac cagcgccagg ccgaccgtcg ccaccagcac cgcgatcccc gccgccgtga
     6241 cggtcgcgaa cacgaccctg gtgaccacgt acgcggcgat gatgccgacg acgatcaccg
     6301 tcccccccat cgtgggcgtt cccatcttct ccatgtgcgt gcgaggcccg tcctcccgga
     6361 tgtgctgtcc ccatccccac acccggaacg cccggatcgc caccggcgtg cccaggaggg
     6421 tgacggccag gcccacggcg gcggccagca ggatggccct caccgcagcg cctccgccag
     6481 ccgctcgagc ccggccacgc gcgagccttt gatcagcacg acatcgccgg cccggagcca
     6541 accgcggagg tcctccacga cctggtccgg gtcctcgtag ctggccacgt cctcgggcag
     6601 ctgtccctcg cggacggcgg agcgggcgat cgatcgggcc tgctccccca ccgtgatcag
     6661 ccggtcgacg cccaggcgca ccaccaactc gccgagccgc tcgtgctcct ccaccgcgat
     6721 cggcccgagc tccgccatgt gtccgagcac ggcgacgagt cgaccctcgc tcgccatcca
     6781 cctcgcggtt cgcaggcccg cggccatcga ctccgggttc gcgttgtagg cgtcgttcac
     6841 aacggacacg ccctgctccg tggtgaaccg ctccatccgc cagcgggaga tccgcgctcc
     6901 cttgagggag gcggcacact ccgcggggga cacgccaaga gcgatcccgc aggccgtcgc
     6961 cgccagcgcg ttcgagacca tgtgcgcacc ggggaccgcc agctccaccg gctcacggtc
     7021 ctcgcccacg accagcacga agctcaccgt tccggagcgg ttgagcacca ggtcgtcggc
     7081 ccggacctcg gcatgatcgc ccgtgccgaa ggtgatcacc cgggccgggg tgcgctcggc
     7141 cagcgcgagc accagcgggt cgtccgcgtt cagcagggcg gtcccgcggg catccagggc
     7201 ctccacgggc tcggcggcgg cctcagtgat ggccccccag gagccgaaga cctccatgtg
     7261 cgccacaccg acattggtga ccacgaccag cgatggcctg gcgatccgcg agaggagagc
     7321 cacgtccccc gcccgtcgag cccccatctc ggccaccagc acctcggtcc ctcgaggggc
     7381 accgagcacg gtcaggggga gtccgatctc gttgttgaac gaggcggggc tggcgtgggt
     7441 ccggaagcgc gaggccagca cggccgcggc caggtccttc gttgaggtct tcccgttcga
     7501 gcccgtgacc ccgatcaccg tcacgtccat cgaggcgcgc tcatcggcgg cgagagccaa
     7561 cagggcctgg ccggtatccc gcacctcaat cgtcgggcca tcggccgaga cggtccgacc
     7621 caccacggcg ccggccgcgc cccgatggag ggcgtccgcg acgaacgcgt ggccgtccgt
     7681 gtgctcgccg ggcagtgcga cgaacaggtc gccggcgcct accctacggc tgtcgatgcc
     7741 gacggaggac acggtggcgt ccgagcctcg gagcctgccg tccgtggcgc gcgccacctc
     7801 ggacagccgt cgcggcctca tcgggttccc atccgtcctc ggagctcctc ggccaccacg
     7861 aggcgatcgt cgaacgggat cgtgcggtcg gcgagctcct ggtaggtctc gtgacccttg
     7921 ccggctatca ccaccacgtc tccgggctgc gcccgctcta ccgccacccg gatcgccgtc
     7981 cgccgatcgg cttcgaccag gtaggggccg ccgcccgccc gggctccagg ctcgatgtcg
     8041 gcgaggatcg ccgcgggttc ctcgctccgc ggattgtcgc tggtcacgac cgtgaggtcg
     8101 gccagcgagg tggcgacctt ccccatcaac ggtcgcttgc cccgatcgcg gtcgccgcca
     8161 cagccgaaca cgacgatcag tcgcccggtc gcgagctgcc gggcggcccg caggacgttc
     8221 tccacgctat ccggcgtgtg cgcgtagtcg accatcacca ggaaaccctg gccctcctcg
     8281 accgtctcca cgcgcccggg gaccccccgc acccgctcga tgccgatcac cgccgctgca
     8341 tcgtcgatcc ccagcgaacg ggaggcggcg agcgcggcca ggcagttctc cacgttgaac
     8401 cggccccgga gcgaggagcg gatcgaccgc ccgtccacac tgaaggcgat gccctccgag
     8461 gtcgctcgga cctccgtggc tcgcacatcg gcccccggct cgatcccgta actcagcgtg
     8521 ggcagcaccc cgtccaacaa ccgacgaccc tcaggactgt cgtggttgac gaccgcgcgg
     8581 tccgagagct cgggcgtgaa cagtcgggcc ttggcctgga agtagtcctc cagtgaggcg
     8641 tgatagtcga ggtgatcctg cgtgaggttg gtgaacaccg cacaccggta ccgcacggcg
     8701 tccacccggt gctggtccag cccgtgcgag gagacctcca tggccacggc ctccacgccc
     8761 cgatccacca tccgccgcag caggcggtgc aggtcgggtg cctcgggggt ggtccgtggg
     8821 aacgggacgg gctcaccatc gatcctgacc ccggtggtgc ccaccacgcc gggaaccagc
     8881 ccggaggcgc ggaagaccga ctcgagcagg taggtggtgg tggtcttgcc gttcgtgccg
     8941 gtgacgccca ccatggtgag gcggtcgctc ggtcgagcga agaacgcggc cgagatcggc
     9001 cccatcgcgc ctcgcaccga cggtaccagc acctgcggca ccgataggtc cagcccccgc
     9061 tccaccacca acgcagaggc tcccaaggcc acggcctcgg ccgcgaagtc gtggccatcg
     9121 gcccgcgatc cggggatgca gaagaacagc gagcccggct ccacctcctg ggaccggtag
     9181 gcgagcgagg tgaccgtggt cgtggggtcg ccgcgcagct cagggccggc gagcgaggtc
     9241 aggagctcgg acagcggacg tgcctcgggg gtgtggggag ccaaatcgcc tgagcttatc
     9301 gttccggcct gcccctgccc ccagccggat ccgctcatgg caccggcagg gcgtggggcg
     9361 gcagaggcac cggcgcgccg ggag
//
LOCUS       DASQQQ010000195         4609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_40445, whole genome shotgun sequence.
ACCESSION   DASQQQ010000195 DASQQQ010000000
VERSION     DASQQQ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40445"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VE646_07520"
     CDS             <1..417
                     /locus_tag="VE646_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HZD79873.1"
                     /translation="TDLEMFRKLLDEVRAGDNVGVLLRGIDKDEVERGQVLARPGSIT
                     PHTNFKAQVYVLSKDEGGRHTPFFNGYRPQFYFRTTDVTGTLNLPEGAEMVMPGDNVE
                     ITVELIAPIAMEEGLRFAIREGGRTVGAGRVTEIIK"
     gene            447..755
                     /gene="rpsJ"
                     /locus_tag="VE646_07525"
     CDS             447..755
                     /gene="rpsJ"
                     /locus_tag="VE646_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HZD79874.1"
                     /translation="MAAPRIRIKLRAYDHRMLDVAAREIVEHVTRTGARVVGPVPLPT
                     ERAIYTVIRSPHKYKDSREHFQMLTHKRLIDIVDATSKTVQELQRLNLSSGVDIEIKL
                     "
     gene            752..1414
                     /gene="rplC"
                     /locus_tag="VE646_07530"
     CDS             752..1414
                     /gene="rplC"
                     /locus_tag="VE646_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018847505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HZD79875.1"
                     /translation="MMAQIKGILGTKVGMTQIFDDTRAIPVTVVKAGPCFVAQVKTAE
                     RDGYRAVQLSFEEPDGRRLTRPMQGHFEAHGAKPGRHVVELRTDDAASYTPGQELRAD
                     LFQPGELTDVVGVSKGRGYAGVMKRHGFGGLGASHGTERKHRAPGAIGACATPSRVFK
                     GMRMAGQMGNERVTVLNLEVVGADPERNLLLLKGAVPGPSGGLVMVRSAVKQAVPTGG
                     GA"
     gene            1414..2061
                     /gene="rplD"
                     /locus_tag="VE646_07535"
     CDS             1414..2061
                     /gene="rplD"
                     /locus_tag="VE646_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HZD79876.1"
                     /translation="MASVPVLDRSGKEVASRDLPAEVFEARVSVPLMHQVVVAGQAAL
                     RRGTHSTKTRGEVAGGGKKPWRQKGTGRARQGSIRAPQWTGGGVVHGPHPRDHSLRVN
                     KKMKRGALRSALTDALQSGKLLVLDELGFERPKTKEAAALLEALGLAGRVLLVLPGPT
                     EDGTVELSFRNLVSVRLAYAPSLGTYEVIAADRVVFTQAAIDALEGTAPSGGEAR"
     gene            2058..2348
                     /gene="rplW"
                     /locus_tag="VE646_07540"
     CDS             2058..2348
                     /gene="rplW"
                     /locus_tag="VE646_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002523451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HZD79877.1"
                     /translation="MKTARDVIIRPVVSEKSYAGLEQNVYTFLVDRRANKTEIKEAIQ
                     AIWNVRVVSVNTLNRRGKVKRQRYTTGKRPDQKRAIVTLAEGDSIEIFETGK"
     gene            2352..3182
                     /gene="rplB"
                     /locus_tag="VE646_07545"
     CDS             2352..3182
                     /gene="rplB"
                     /locus_tag="VE646_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HZD79878.1"
                     /translation="MAVRKYKPTTPGRRGASVSSFEEITRAKPEKSLVTKGRKRAGRN
                     THGRITARHQGGGNKRRYRVIDFRRDKDGVPAKVAHIEYDPNRSSRIALLHYVDGEKR
                     YILAPQGLGLGDRVLSGDGADIRSGNALPLRNIPVGTTVHAVELKPGAGARMARSAGT
                     SIQVMAKEGRYATLRLPSGETRMVEATCKATVGEVGNAEHELIQLGKAGRNRWLGKRP
                     SVRGVAMNPVDHPLGGGEGKSSGGRHPTSPWGKKEGRTRKKNKASNRYIVRRRGKGRG
                     "
     gene            3186..3467
                     /gene="rpsS"
                     /locus_tag="VE646_07550"
     CDS             3186..3467
                     /gene="rpsS"
                     /locus_tag="VE646_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HZD79879.1"
                     /translation="MPRSIKKGPFVDEHLMVRVADMNRRGEKKVIRTWSRRSTIVPEM
                     IGHTIAVHDGRKHVPVYISESMVGHKLGEFASTRTFRSHARSERSSSVR"
     gene            3471..3815
                     /gene="rplV"
                     /locus_tag="VE646_07555"
     CDS             3471..3815
                     /gene="rplV"
                     /locus_tag="VE646_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HZD79880.1"
                     /translation="MEAKAQVRYVRISPPKARRVIDLIRGQQVDEARRILRFSPLGAT
                     KTIEKCLNSAVANAEQVPGVAPQNLVVDRAWVDEGPTLKRWRPRAYGRAARVRKRTAH
                     ITVIVKSLGEEA"
     gene            3815..>4609
                     /gene="rpsC"
                     /locus_tag="VE646_07560"
     CDS             3815..>4609
                     /gene="rpsC"
                     /locus_tag="VE646_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010986347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HZD79881.1"
                     /translation="MGHKVNPYGFRLGVIYPWKSNWYAGRDYAEALHQDVWIRRHIRS
                     RLSRAGISSIDIERKGDQIWVFIRTARPGIVIGRKGAEVDRIRKDVERYTRKRVDVKV
                     EDMNSAASELRPETDATLLAQGVAEQLAGRVSFRRAMRRAVQTAMRSGALGVRVQCGG
                     RLGGTEMSRREWYREGRVPLHTLRARIDFGTAEANTTFGQIGVKVWVYHGDEIPQAEQ
                     ETERARARALAQVSRGGGASTGALITDEREAAEVEEPAERQAPAAEP"
BASE COUNT          765 a         1578 c         1714 g          552 t
ORIGIN      
        1 accgacctgg agatgttccg caagctcctg gacgaggtcc gggccgggga caacgtgggg
       61 gtgctgctgc gggggatcga caaggacgag gtggagcggg gccaggtgct ggcccgcccc
      121 ggctcgatca ccccccacac caacttcaag gcccaggtct acgtgctgag taaggacgag
      181 gggggacggc acaccccgtt cttcaacggc taccgccccc agttctactt ccgcaccacc
      241 gacgtcaccg gcaccctcaa cctgcccgag ggcgcggaga tggtgatgcc cggcgacaac
      301 gtggagatca ccgtggagct gatcgccccc atcgccatgg aggagggcct gcgcttcgcg
      361 atccgcgagg gcggccgcac cgtgggtgcc gggcgcgtca ccgagatcat caagtaggga
      421 cgagagggaa gggaagcgcg agggacgtgg ccgcaccgag gatcaggatc aagctccgcg
      481 cctacgacca ccggatgctg gacgtggcgg ctcgcgagat cgtcgagcac gtcacgcgga
      541 ccggcgcccg ggtcgtgggt cccgtccccc tgcccaccga gcgggccatc tacaccgtga
      601 tccgctcgcc ccacaagtac aaggactcgc gggagcactt ccagatgctc acgcacaagc
      661 ggctcatcga catcgtggac gcgacctcca agacggtcca ggagctccag cgcctgaacc
      721 tgtcctcggg ggtggacatc gagatcaagc tctgatggcg cagatcaagg gcatcctggg
      781 gaccaaggtc ggcatgaccc agatcttcga cgacacgcgg gccatcccgg tgaccgtcgt
      841 caaggccggg ccgtgcttcg tggcgcaggt caagaccgcc gagcgcgacg gctaccgggc
      901 cgtccagctg agcttcgagg agcccgacgg ccgtcgcctg acccgcccca tgcaggggca
      961 cttcgaggcc cacggggcca agcccgggcg ccacgtggtg gagctccgca ccgacgacgc
     1021 cgcctcctac acgccgggcc aggagctgcg cgccgacctg ttccagcccg gggagctgac
     1081 cgacgtggtg ggcgtgtcca agggccgggg ctacgccggc gtgatgaagc ggcacgggtt
     1141 cggggggctg ggcgccagcc acggcaccga gcgcaagcac cgcgcgcccg gcgccatcgg
     1201 ggcctgcgcc acgcccagcc gcgtgttcaa gggcatgcgg atggccggcc agatgggcaa
     1261 cgagcgcgtc accgtcttga acctcgaggt ggtcggggcc gaccccgagc gcaacctgct
     1321 gctgctgaag ggcgccgtcc ccggaccgtc cggcggcctg gtgatggtcc gctccgcggt
     1381 gaagcaggcc gtgccgaccg gaggtggtgc ctgatggcgt cggtgccggt gctggaccgc
     1441 tcgggcaagg aggtggccag ccgcgacctg ccggccgagg tgttcgaggc tcgggtcagc
     1501 gtgcccctga tgcaccaggt ggtggtggcg ggccaggcgg ccctgcgccg cggcacgcac
     1561 tccacgaaga cccggggcga ggtggccggg ggcgggaaga agccctggcg gcagaagggc
     1621 accggccggg cccggcaggg ctcgatccgg gcgccgcagt ggaccggcgg gggcgtggtg
     1681 cacggcccgc acccccggga ccacagcctg cgcgtgaaca agaagatgaa gcgcggggcg
     1741 ctgcgctcgg cgctcaccga cgccctgcag agcggcaagc tcctggtcct ggacgagctg
     1801 ggcttcgagc ggcccaagac caaggaggcc gcggccctgc tggaggccct gggcctggcc
     1861 ggcagggtcc tgctggtcct gcccgggccg accgaggacg gcaccgtgga gctgtcgttc
     1921 cgcaacctgg tgagcgtccg cctggcctac gccccgtccc tcggcaccta cgaggtgatc
     1981 gccgccgacc gcgtggtgtt cacgcaggcc gccatcgacg cgctggaggg cacggcccca
     2041 tcggggggtg aggcccggtg aagaccgcgc gcgacgtgat catccgcccg gtggtctccg
     2101 agaagtccta cgcgggcctg gagcagaacg tctacacgtt cctggtggac cggcgggcca
     2161 acaagaccga gatcaaggag gcgatccagg cgatctggaa cgtccgggtg gtctcggtga
     2221 acaccctgaa ccgccggggc aaggtgaagc gccagcgcta caccaccggc aagcgccccg
     2281 accagaagcg cgccatcgtc accctcgccg agggcgactc gatcgagatc ttcgagaccg
     2341 ggaagtagcc catggccgtt cgcaagtaca agccgaccac gcccggacgc cgcggcgcca
     2401 gcgtgtcctc cttcgaggag atcacgcggg ccaagcccga gaagtcgctg gtgaccaagg
     2461 gccgcaagcg cgccgggcgc aacacgcacg ggaggatcac cgcccggcac cagggcggcg
     2521 gcaacaagcg ccggtaccgg gtgatcgact tccgccggga caaggacggc gtgccggcca
     2581 aggtggccca catcgagtac gacccgaacc gctcgtcgcg catcgcgctg ctgcactacg
     2641 tggacggtga gaagcgctac atcctggccc cgcagggcct gggcctgggc gaccgggtgc
     2701 tctcggggga tggcgcggac atccgctcgg gcaacgcgct gcccctccgg aacatcccgg
     2761 tgggcaccac ggtgcatgcg gtggagctga agccgggggc cggggcccgc atggcccgct
     2821 cggccggcac cagcatccag gtcatggcca aggaaggccg gtacgcgacg ctgcggctgc
     2881 cctcgggcga gacgcggatg gtcgaggcca cctgcaaggc cacggtgggc gaggtgggca
     2941 acgccgagca cgagctgatc cagctcggca aggccggacg caaccggtgg ctgggcaagc
     3001 ggccctcggt ccgcggcgtg gccatgaacc cggtcgacca ccccctgggc gggggcgaag
     3061 gcaagagctc gggcggccgc caccccacgt cgccgtgggg caagaaggag ggccgaacgc
     3121 gcaagaagaa caaggcgtcg aaccgctaca tcgtgcggcg ccgcgggaag gggcgtggct
     3181 agccgatgcc gaggtccatc aagaagggtc cgttcgtgga cgagcacctg atggtcaggg
     3241 tcgcggatat gaaccggcgg ggcgagaaga aggtcatcag aacctggtcc cgccgctcga
     3301 cgatcgtgcc ggagatgatc gggcacacca tcgccgtcca cgacgggcgc aagcacgtcc
     3361 ccgtgtacat ctcggagtcg atggtggggc acaagcttgg cgagttcgcc tccacccgga
     3421 ccttccggtc ccacgcgcgc tccgagcgga gcagctcggt caggtaggcg atggaagcga
     3481 aggcgcaggt caggtacgtg cggatctccc cgcccaaggc ccggcgggtg atcgacctca
     3541 tccgggggca gcaggtggac gaggcccgcc ggatcctgcg cttctcgccg ctgggcgcga
     3601 ccaagaccat cgagaagtgc ctgaactcgg ccgtggccaa cgccgagcag gtgcccggcg
     3661 tggcgcccca gaacctggtg gtggaccggg cctgggtgga cgaggggccc accctcaagc
     3721 ggtggcgccc ccgggcctac ggccgggccg cgcgggtccg caagcgcacc gcgcacatca
     3781 ccgtgatcgt gaagtcgttg ggagaggaag cgtagtggga cacaaggtca acccgtacgg
     3841 gttccggctg ggggtcatct acccctggaa gtccaactgg tacgccggcc gcgactacgc
     3901 ggaggcgctc caccaggacg tctggatccg ccgccacatc cgctcgcggc tgtcccgggc
     3961 cgggatctcc agcatcgaca tcgagcgcaa gggcgaccag atctgggtct tcatccggac
     4021 cgcccggccc ggcatcgtga tcgggcgcaa gggggccgag gtcgaccgga tccgcaagga
     4081 cgtggagcgc tacacccgca agcgcgtgga cgtgaaggtc gaggacatga actcggcggc
     4141 cagcgagctg cggcccgaga ccgacgccac gctgctggcc cagggcgtgg ccgagcagct
     4201 ggccggccgc gtgtcgttcc ggcgggccat gcgtcgggcg gtgcagaccg ccatgcgctc
     4261 gggcgccctg ggcgtccggg tgcagtgcgg cggccgcctg ggcggcaccg agatgtcgcg
     4321 gcgcgagtgg taccgcgagg gccgtgtgcc gctgcacacg ctccgggcgc ggatcgactt
     4381 cggcaccgcc gaggccaaca ccaccttcgg tcagatcggg gtgaaggtgt gggtctacca
     4441 cggtgacgag atcccgcagg ccgagcagga gaccgagcgg gcccgggccc gagccctggc
     4501 gcaggtctcc cgcggcggcg gggcgtccac cggcgcgctg atcaccgacg aacgggaggc
     4561 cgccgaggtg gaggagcccg ccgagcggca ggcccccgcg gccgagccg
//
LOCUS       DASQQQ010000196         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_40448, whole genome shotgun sequence.
ACCESSION   DASQQQ010000196 DASQQQ010000000
VERSION     DASQQQ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40448"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1248)
                     /locus_tag="VE646_07565"
     CDS             complement(61..1248)
                     /locus_tag="VE646_07565"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HZD79882.1"
                     /translation="MAGGLDYLKDELRQLEEQGLLIRERVLEGPTGARARYDGREVIN
                     LASNNYLGLAGHPRMRAAASRAAQELGAGSGAVRTIAGTMAMHRDLERRFADFKRAED
                     ALMFQSGFTANAGTVAAVLTKEDVIVSDQLNHASIIDGARLSRAEIKVFPHKDPGAAD
                     RLLAETARPGRRQLLITDGVFSMDGDIAPLPDLVEVAERHGAIMMVDDAHASGVLGNG
                     GAGTVDHFGLHGRVDIQVGTLSKAIGALGGFIAGPRHLIRWLQNRGRPYLFSTSAPPA
                     VVAACMEALDIIRDEPERLERLRSNTRSFKEGLHELGFDTGASETPITPVITGDEEKT
                     QEFARRLFEEGVFTPAIVYPTVARGQARVRTIVIADHTEEDLREALDVFERVGKELDL
                     LPA"
     gene            1302..1790
                     /locus_tag="VE646_07570"
     CDS             1302..1790
                     /locus_tag="VE646_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79883.1"
                     /translation="MGLIEVDGFLADSVVRAEGKLYVQGAGWNRITAPLFPVVHDRIG
                     VGLIFRVAAEPAAQTYRFDLRLEDEQGREQVLGMTRDPAGGDQGKLYRLTGEFTVGPS
                     SASDDSLVAMAVNLNGMTFERPTAYRFVVDVDGVDVKAMPFRVEPLAAQSPPVTGGGG
                     YL"
     gene            complement(1815..>2587)
                     /locus_tag="VE646_07575"
     CDS             complement(1815..>2587)
                     /locus_tag="VE646_07575"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZD79884.1"
                     /translation="TCRTGRAHICENLRILGVDIDEVFAEYVVIPAANAWIVGEGIDP
                     DVASIMEPFGNGVHAAFGTEGGEDLATNPVAVIGCGPIGLFAVGIARAAGAWKVMAIE
                     PNDDRRALAAKMGADLLIDPKADDPVEAVLAATNGTGAEVVLEMSGNPTAIDQGTRML
                     ARGGRMSLLGLPDGPVTLDLNDQVIFKEARVQGITGREMFRTWQQTTTLLSTGRVDVT
                     PVITHRFPMERFEEAFETTASGRAAKVIMFPARGDGPG"
BASE COUNT          360 a          948 c          869 g          410 t
ORIGIN      
        1 ctgctgctcg agggtctcca gggcaagatc cgccggtccg agcccacctg aggcggttcg
       61 tcaggcgggg agtaggtcga gctccttgcc cacccgctcg aagacgtcca gcgcttcccg
      121 cagatcctcc tcggtgtggt ccgcgatcac gatggtccgc acgcgcgcct gcccccgggc
      181 caccgtcgga tacacgatcg cgggggtgaa caccccttcc tcgaacagcc gccgcgcgaa
      241 ctcctgcgtc ttctcctcgt ctccggtgat gacgggcgtg atgggcgtct cgctggcccc
      301 ggtgtcgaag ccgagctcat gcagcccctc cttgaaggac cgggtgttgg agcggagccg
      361 ctccagccgc tccggctcgt cacggatgat gtcgagggcc tccatgcacg ccgccaccac
      421 ggccggcgga gcgctcgtgg agaacaggta ggggcggccc cggttctgca gccagcggat
      481 cagatgcctg ggtccggcga tgaaccctcc cagcgccccg atcgccttgg acagcgtgcc
      541 cacctggata tccacccgtc cgtgcaggcc gaagtggtcc accgtgcccg cccccccgtt
      601 cccgagcacc ccggaggcgt gggcgtcgtc caccatcatg atcgccccgt gccgttcggc
      661 cacctccacc aggtcgggga gcggcgcgat gtccccgtcc atcgagaaca cgccgtcggt
      721 gatcagcagc tgcctccgtc ccggtcgggc ggtctccgcc aggagccggt cggccgcccc
      781 gggatccttg tgggggaaga ccttgatctc ggccctagac aaccgggccc catcgatgat
      841 cgaggcgtgg ttcagctggt ccgagacgat cacgtcctcc ttggtgagga cggcggccac
      901 cgtccccgcg ttcgccgtga aacccgattg gaacatcagg gcgtcctcgg cgcgcttgaa
      961 gtccgcgaac cggcgctcca ggtcgcggtg catggccatc gtccccgcga tggtccggac
     1021 ggccccgctg cccgcaccga gctcctgggc ggctcgggac gctgcggccc gcatccgagg
     1081 gtggcccgcc aggcccaggt agttgttcga ggccaggttg atgacctcac gaccgtcgta
     1141 gcgagcgcgg gccccggtgg gtccctccag gacccgttcc cggatcagca acccctgctc
     1201 ctcgagctgc cggagttcgt ccttcaggta gtcgagcccg ccggccatgt ccgccctcac
     1261 gctaccgcat taggctggga tgggcagaca cgggaggtcg cgtggggctg atcgaggtgg
     1321 acgggttcct ggcggactcc gtggtgcggg ccgagggcaa gctgtacgtg cagggcgcgg
     1381 gctggaaccg catcaccgcc cccctgttcc cggtcgtgca cgaccggatc ggcgtgggat
     1441 tgatcttccg ggtggccgcc gagcccgccg cccagaccta ccgcttcgat ctgcggctcg
     1501 aggacgagca gggacgcgag caggtgctgg ggatgacccg ggacccggcg ggcggcgacc
     1561 agggcaagct gtaccggttg accggtgagt tcaccgtggg cccctcctcg gccagcgacg
     1621 acagcctggt ggccatggct gtcaacctga acggcatgac cttcgagcga ccgaccgcct
     1681 accgcttcgt ggtggatgtg gacggcgtcg acgtgaaggc gatgccgttc cgcgtggagc
     1741 cgctcgcggc gcagtccccg ccggtgaccg ggggcggcgg ctacctctga tccctcgacc
     1801 ggacgaccgg cccgctagcc cggcccgtcg cccctggccg ggaacatgat gaccttggcg
     1861 gcccgtccgg aagccgtcgt ctcgaacgcc tcctcgaacc gctccatggg gaagcggtgg
     1921 gtgatcaccg gggtgacgtc tacccggccc gtggagagca acgtcgtggt ctgctgccag
     1981 gtgcggaaca tctcccgccc ggtgatcccc tggacccggg cctccttgaa gatcacctgg
     2041 tcgttcaggt ccagggtgac ggggccatcg ggcaggccga gcagcgacat ccgccccccg
     2101 cgcgccagca tccgggtccc ctgatcgatc gccgtcggat tgcccgacat ctccaggacc
     2161 acctccgccc cggtcccgtt ggtcgcggcc agcaccgcct cgaccggatc gtccgccttg
     2221 gggtcgatca gcaggtcggc ccccatcttc gcggccaggg cgcggcggtc gtcgttcggc
     2281 tcgatcgcca tcaccttcca ggcaccggcc gcgcgggcga tccccacggc gaacaggccg
     2341 atcggtccgc atccgatcac cgccaccggg ttcgtggcca gatcctcgcc tccctcggtg
     2401 ccgaaggcag cgtgcacgcc gttgccgaac ggctccatga tcgaggcgac gtctgggtcg
     2461 atcccctccc ccacgatcca ggcgttggcg gcggggatca ccacgtactc cgcgaagacc
     2521 tcatcgatat ccacccccag gatccgcagg ttctcgcaga tgtgcgcccg tccggtccgg
     2581 caggtgg
//
LOCUS       DASQQQ010000197         3420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_40484, whole genome shotgun sequence.
ACCESSION   DASQQQ010000197 DASQQQ010000000
VERSION     DASQQQ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40484"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1131)
                     /gene="pilM"
                     /locus_tag="VE646_07580"
     CDS             complement(46..1131)
                     /gene="pilM"
                     /locus_tag="VE646_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HZD79885.1"
                     /translation="MARTRIGLDVGSTAVRVAEVSMGDVPVVVRAAQVPLPPGAVEAG
                     EIRQPELVTDALRELWSSSGVKGKQVYLGVGNQRVVVREVALPWLPERELRDSLAFQV
                     QEFIPMAADEAVLDYDPLGEMDQGGRRMQRILLVAAHKAMVNALVQAVLAAKLEPLGI
                     DLTPFAVVRAVGAGDEGLDLESPGDEAVIDIGAHVTSICVHDRGVTRFVRILPSGGRD
                     ITLAIARGLAVDDGVAEALKRGEPVEPPFDQEGRPMPLPSAEDVRRLALSRAGSFVDE
                     VRSSLEFYAAQVPGARIARVVVTGGGSRLDGFLQLLQERIPVVVDRGRLFERVKSELN
                     LTAEAQAEAEAVLSVAVGLAIAERRRP"
     gene            complement(1132..1338)
                     /locus_tag="VE646_07585"
     CDS             complement(1132..1338)
                     /locus_tag="VE646_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZD79886.1"
                     /translation="MGTSRSGRQAGDRLLTVAEVAATMRVSTMTVYRMIKGGELPAIR
                     VGKNYRIRESDVDQYLYDRQVEGA"
     gene            complement(1349..1501)
                     /locus_tag="VE646_07590"
                     /pseudo
     CDS             complement(1349..1501)
                     /locus_tag="VE646_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016437488.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
     gene            complement(1764..2552)
                     /locus_tag="VE646_07595"
     CDS             complement(1764..2552)
                     /locus_tag="VE646_07595"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HZD79887.1"
                     /translation="MTGYDVALRILIALPAGLAFGSFLTVAIHRVPAGESLVRPRSRC
                     PSCGTPIRNVDNVPVVSWLVLRGRCRACGARISAVYPLTELTTGILFVAVALRFEDVW
                     GAVLLAPFLGLMVALTVIDWRHKIIPNRLVYPAIVLTVVYVTVADLAGGGLDAVRAGV
                     GFLAYGLGLLVIALISPRGMGMGDVKLAALVGLALGGLGLRYVAVAAGAGILVGGVAA
                     IVALLTGAGRKAAMPYGPSIAVGAAVAVFAGAELAEAYLRVVGA"
     gene            complement(2599..>3420)
                     /locus_tag="VE646_07600"
     CDS             complement(2599..>3420)
                     /locus_tag="VE646_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79888.1"
                     /translation="SLHLYGELWANGSILVDQPQAYIDFDAKSTTSSVTVSKGTVFGD
                     AYYCTGTPPDNVAGQELQTCGLGPPPTNPFPQIQYYPDLWADAGYFLLTPFTGATACT
                     LAKAYVEGTAAGTFQGGLGVPAGYSGVVVYIDSTCTYTSSNNATISLGKDLAVITKGS
                     VNLSQRSSWNGVGSMRNLHLMSPWPSTGSPSCPTQNITVGNNTSFNSLVRTFVYTPCT
                     ATMLNNNSAFQGQVVGTNVVIGNLFNMTYKPIFVPGADIVGFEQDIAYIREVQSP"
BASE COUNT          541 a         1261 c         1127 g          491 t
ORIGIN      
        1 gcacctgccg ctggtggagc tcggggggaa ggaggttgac ctggctcacg gacgcctccg
       61 ctcggcgatg gccagcccga cggccaccga caggacggcc tcggcctcgg cctgcgcctc
      121 ggcggtcagg ttgagctccg acttcacgcg ctcgaagagc ctgccccggt cgacgaccac
      181 cgggatccgc tcctggagga gctggaggaa gccgtccagc cgtgaaccgc cgccggtgac
      241 gaccacccgc gcgatccggg ccccggggac ctgcgccgcg tagaactcga gggaggagcg
      301 gacctcgtcc acgaagcttc ccgcgcgcga cagcgccagc cgtcggacgt cctccgccga
      361 cgggagcggc atcgggcgcc cctcctgatc gaacgggggc tccacgggct cgccacgctt
      421 gagcgcctcc gcgacgccgt cgtccacggc cagcccgcgc gcgatcgcga gggtgatgtc
      481 gcggccgccc gaaggcagga tccgcacgaa ccgcgtcacc ccccggtcgt gcacgcagat
      541 gctggtcacg tgggctccga tgtcgatcac ggcctcgtcg cccggggact cgaggtccag
      601 gccctcgtcg ccggccccga cggcccggac gacggcgaag ggggtgaggt cgatgcccag
      661 cggctcgagc ttggccgcca ggaccgcctg cacgagcgcg ttcaccatcg ccttgtgcgc
      721 ggcgaccagc aggatccgct gcatccgtcg gccgccctgg tccatctcgc ccagggggtc
      781 gtagtcgagc acggcctcgt cggccgccat ggggatgaac tcctggacct ggaacgccag
      841 cgagtcacgg agctccctct ccgggagcca cggcagggcc acctcgcgga ccacgacccg
      901 ctggttgccc acgccgaggt acacctgctt gcccttgacg ccgctgctgc tccagagctc
      961 gcggagggcg tcggtgacca gctcgggctg ccggatctcc cccgcctcga ccgcgcccgg
     1021 aggcagcgga acctgcgcgg cccggaccac gaccggcacg tcgcccatcg agacctccgc
     1081 gacccgcacc gcggtggagc cgacgtccag gccgatcctg gtcctggcca tctacgcccc
     1141 ctccacctgg cggtcgtaca ggtactggtc gacgtcggac tcccggatcc ggtagttctt
     1201 ccccaccctg atcgccggga gctccccgcc tttgatcatc cggtagacgg tcatcgtcga
     1261 gacccgcatc gtcgccgcga cctcggccac ggtgagcagg cggtcgccgg cctgccgacc
     1321 gctccgagag gtaccgatcc acgtcggact cccggatccg gtagttcttg cccaccctga
     1381 tcgcggccag ctgcccgctc ttgatcagcc ggtacacggt catgttcgac acgcgcatcg
     1441 tggccgccac ctcacctacc gtgaggagcc ggtcacccac gaacggccgc cgcttcccca
     1501 tctttcacaa ctcccccatc cggcccatat ccctcatgtt ccacatctgc cacatctgcc
     1561 tcgtatccac cctcgtactc tggggctccg caccgtaaca agtgcaacat ctggcgtcaa
     1621 tcaccccttc tacctacttt cggcacgaca ggctaaatta cacatatgta attagctcta
     1681 cttctcagga gtcacacgcg caacacgacg ccaccgagct cagcacaagg gggcacgtgg
     1741 atgagggccg acgacccgga ggatcaggcg ccgacgaccc ggaggtacgc ctcggccagc
     1801 tccgcccccg cgaagacggc gaccgcggca cccacggcga tcgaggggcc gtagggcatg
     1861 gccgccttgc gcccggcacc ggtgagcagg gcgacgatgg ccgcaactcc cccgaccagg
     1921 atccccgcgc cggccgcgac cgcgacgtac cgcaggccca ggccgccgag cgccaggccg
     1981 acgagggcgg cgagcttcac gtcgcccatc cccatccccc tcggcgagat aagcgcgatc
     2041 accaggagcc cgagcccgta cgccaagaac cccacgccgg cccggacggc gtcgagtccc
     2101 ccgccggcca ggtcggcgac cgtcacgtac acgacggtca acacgatcgc ggggtagacg
     2161 agccggttcg ggatgatctt gtggcgccag tcgatcacgg tgagggccac catgagcccg
     2221 aggaacggcg ccaacagcac cgccccccag acgtcctcga acctcagggc caccgcgacg
     2281 aacaggatcc ccgtggtgag ctcggtcagg gggtagacgg cagagatcct cgcgccgcac
     2341 gcgcggcacc gcccccggag caccagccac gacacgacgg gcacgttgtc gacgttccgg
     2401 atcggcgtcc cgcagctcgg gcagcgtgag cggggccgga ccagcgactc ccccgccggg
     2461 acccggtgga tcgcgaccgt caggaaactc ccgaaggcga gcccggcggg cagcgcgatc
     2521 aggatccgga gcgcgacgtc gtatcccgtc atggtgtccg cggaacgcta ccgcagtcgc
     2581 ccgcccgcgg cgggcggcct acgggctctg gacctcgcgg atgtaggcga tgtcctgctc
     2641 gaacccgacg atgtcagccc ccgggacgaa gatcggcttg taggtcatgt tgaacaggtt
     2701 gccgatcacc acgttggtcc cgaccacctg accctggaac gcggagttgt tgttcagcat
     2761 cgtcgccgtg cacggcgtgt acacgaacgt gcggaccagg ctgttgaagc tggtgttgtt
     2821 ccccacggtg atgttctggg tggggcagga cggcgagccg gtcgacggcc aggggctcat
     2881 caggtggagg ttccgcatcg agcccacccc gttccagctc gagcgctggc tgaggttgac
     2941 cgaccccttc gtgatgaccg ccaggtcctt gccgagcgat atcgtcgcgt tgttgctgct
     3001 cgtgtaggtg caggtcgagt cgatgtacac gacgacgccg ctgtagccgg ccggcacgcc
     3061 cagcccgccc tggaacgttc cggccgccgt cccctcgacg taggccttcg ccagcgtgca
     3121 ggcggtggcg cccgtgaacg gcgtcagcag gaagtagccg gcgtcggccc acaggtccgg
     3181 gtagtactgg atctggggga acgggttcgt gggcggcggc cccaggccgc aggtctgcag
     3241 ctcctggccg gccacgttgt cgggcggggt ccccgtgcag tagtaggcgt cgccgaagac
     3301 ggtccccttg ctcaccgtga ccgaggaggt ggtcgacttg gcatcgaagt cgatgtaggc
     3361 ctgcggctgg tccacgagga tcgagccgtt cgcccagagc tccccgtaca ggtgcaggct
//
LOCUS       DASQQQ010000198         2937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_344523, whole genome shotgun sequence.
ACCESSION   DASQQQ010000198 DASQQQ010000000
VERSION     DASQQQ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344523"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VE646_07605"
     CDS             complement(<1..222)
                     /locus_tag="VE646_07605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZD79889.1"
                     /translation="MSATVLFAGGGTGGHLYPALNIAAAMRAARPTMEPVFVGARRGV
                     EARVLPEKGVEYRLLPLQPIRRDRVWQNWR"
     gene            complement(219..1334)
                     /gene="ftsW"
                     /locus_tag="VE646_07610"
     CDS             complement(219..1334)
                     /gene="ftsW"
                     /locus_tag="VE646_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HZD79890.1"
                     /translation="MLLLSVTLVAVGLIMVYSASAVMAQSRGLVPHYFLLRQMSGALA
                     GMVALVVAAQVDYRRLRTLAWPLLAVVVGMLIAMVLPGMEHLAPEANGARRWLMIGPV
                     GIQPSELAKLVLIIWTAALVVKKQDRLRSLSKGLAPFLLIWGLVVALILAQPDLSTAA
                     LALLLAGLVAYAGGARPAHFVALGLLATPFLWSQVTRVAYRMERITAFLDPRADVSDL
                     GYQINQSLIALGSGGVLGRGFGRGQQKFGFLPEPHNDFILAMIGEEWGFLGVAVLTLL
                     FTVFALVGYRIARNAADLFGFLLAVGITNLVVVQAFLHMAVNLALVPPTGITLPFISY
                     GRSSLIVTLAGVGMLMSIARISERRDRALAKARGRGA"
     gene            complement(1447..2835)
                     /gene="murD"
                     /locus_tag="VE646_07615"
     CDS             complement(1447..2835)
                     /gene="murD"
                     /locus_tag="VE646_07615"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HZD79891.1"
                     /translation="MIEPRRIGILGLGRSGRSAAKLALAAGSAVFASDTGDSEEVRQS
                     AAEIERLGGRAETGGHTVARLAECDLLVVSPGIPPTLPLLHDDGLAGVPWTSELEFAY
                     RHLRAPVIAVTGTNGKTTVTAWAAHVLREAGCEVVAAGNIGLPLSEVALRPDPPDWVV
                     AEASSYQLGRIDTFAPKVGVVTNLAPDHLDRYPDLASYYSDKAHLFDNASRESTWVLN
                     AEDPNVLALPGDAPGRRLVFRAEGPLQPGEQGAYVDADGMLRIWVAGEDGFLVGQEEL
                     RLLGAHNRANALATALAAYAVIAEPVALRPGLRSFAPLSHRLEPVAEIEGVLWVNDSK
                     ATNVASARVAVRSMDRPTVLLVGGRHKGERYGPLGAALAGRVKAVVAYGEAADRLAAE
                     LSDRAHVEVVRGGFEAVVDRAATLAEAGDAVLLAPACASFDMFRDYEERGERFRRLVE
                     SRSSRGRGVSRG"
BASE COUNT          470 a         1172 c          921 g          374 t
ORIGIN      
        1 ccgccagttc tgccacaccc gatcccgccg gatcggttgg aggggcagca gccgatactc
       61 cactcccttt tcgggtagca cccgggcctc cactccccgg cgagcgccca cgaacaccgg
      121 ctccatggtg ggtcgcgcgg cccgcatcgc cgcggcgatg ttcaacgccg ggtacagatg
      181 cccgcccgtc ccaccgccgg cgaacagcac cgtcgcgctc acgcgcccct cccccgcgcc
      241 ttcgccagcg cgcggtcccg ccgctcgctg atacgggcga tgctcatgag catgcccacg
      301 cccgccagcg tcacgatcag gctggagcgg ccgtacgaga tgaacgggag cgtgatcccc
      361 gtgggcggaa ccagcgccag gttcacggcc atgtgcagga acgcctggac taccaccagg
      421 ttggtgatcc cgaccgccag caggaagccg aagaggtccg ccgcgttccg cgcgatccgg
      481 tagcccacca gcgcgaagac ggtgaagagg agcgtgagca cggccacgcc caggaacccc
      541 cactcctccc cgatcatcgc caggatgaag tcgttgtgcg gctcggggag gaagccgaac
      601 ttctgctgac cgcgcccgaa cccccgcccc aggacgcccc cactcccgag cgcgatcagc
      661 gactggttga tctggtagcc gaggtcgctc acgtcggccc gcggatcgag gaatgccgtg
      721 attcgctcca tccggtacgc cacccgcgtc acctgggacc acaggaacgg cgtagcgagc
      781 aagccgaggg cgacgaagtg ggcgggccgt gccccacccg catacgccac cagccccgcg
      841 agcagaagcg cgagtgccgc cgtggagagg tccggctgcg ccaggatcag cgcgaccacc
      901 agcccccaga tcaggaggaa cggcgccagc cccttgctca ggctccgcag ccggtcctgc
      961 ttcttcacca ccagcgccgc cgtccagatg atcaggacca gcttggccag ctccgacggc
     1021 tggatcccca ccgggccgat catgagccac cgtcgcgccc cgttcgcttc cggcgccaga
     1081 tgctccattc ccggcagcac catcgcgatc agcatcccca ccacgaccgc gagcagcggc
     1141 cacgcgaggg tgcggagccg ccggtaatcc acctgcgcgg ccaccaccag ggccaccatc
     1201 ccggccagcg cgcccgacat ctggcgcagc aggaagtagt ggggcaccag gccgcgcgac
     1261 tgcgccatga ccgcgctcgc gctgtagacc atgatcagcc ccaccgccac gagcgtcacc
     1321 gaaaggagca gcaccgcacc gctctcccat cccctcccga gtcggctcgg gtcctgcacc
     1381 gcccgctggc gcggatacgg gtccgccggg accgtgcggc cacggccgat cgtcgtcacg
     1441 cccgccctag ccacgcgaca ccccacgccc ccgtgaggaa cgggattcca ccaaccggcg
     1501 gaaccgctcg ccgcgctcct cgtagtcgcg gaacatgtcg aagctcgcgc atgccggcgc
     1561 caggagaacc gcgtccccgg cttccgcgag tgtcgcggcg cggtcgacca ccgcttcgaa
     1621 gccaccacgg accacctcca cgtgcgcccg gtccgatagc tccgccgcga gacggtctgc
     1681 cgcttccccg taggcgacca cggccttgac gcgccctgcc agtgccgcac cgagcggtcc
     1741 gtaccgctcg cccttgtgcc gcccgcccac cagcagcacg gtcggtcggt ccatgctccg
     1801 gacggcgacc cgggccgagg cgacgttcgt ggccttcgag tcgttcaccc acagcacgcc
     1861 ctcgatctca gccacgggct ccagccggtg cgagagcggg gcgaaggagc ggagccccgg
     1921 gcggagggcc accggctcgg cgatcacggc gtatgcggcg agggcggtgg ccagcgcgtt
     1981 ggcccggttg tgagcgccca gcaacctcag ctcctcctgg ccgacgagga acccgtcctc
     2041 ccccgccacc cagatgcgga gcatgccatc cgcatccacg tacgcccctt gctcccccgg
     2101 ctgaagcggg ccctcggccc ggaacaccag ccgccgcccg ggagcatcgc ccggcagcgc
     2161 cagcacgttc gggtcctcgg cgttcagaac ccacgtgctc tcccgcgacg cgttgtcgaa
     2221 caggtgcgcc ttgtcgctgt agtagctcgc gaggtccggg taccggtcca gatggtccgg
     2281 cgccaggttc gtcaccacgc ccaccttcgg cgcgaacgtg tcgatccggc cgagctgata
     2341 ggagctggcc tccgccacga cccagtccgg ggggtccgga cgcagcgcga cctccgaaag
     2401 cggcagcccg atgttgcccg cggctacgac ctcgcacccc gcctcccgga gcacgtgggc
     2461 cgcccacgcc gtcacggtgg tcttgccgtt cgtccccgtc acggcgatca cgggggcccg
     2521 caagtgccga taggcgaact cgagctccga cgtccacggg accccggcga ggccgtcatc
     2581 gtgcagcagc gggagggtcg gcgggatgcc cgggctcacc accaacaggt cgcactcggc
     2641 cagccgggca accgtatgcc cgcccgtttc cgcccgcccc cccaaccgct cgatctccgc
     2701 cgccgactgc cgcacctcct cgctatcgcc ggtatccgat gcgaagacag cgctgcccgc
     2761 ggcgagcgcc agcttcgccg ccgaccggcc gctccggccc agaccgagga tcccgatccg
     2821 ccgtggctcg atcatcggat cttgagggtg ctgaaggcca cgagcgcgca caggatcccc
     2881 aggatccaga agcggatgat caccttgctc tccgcccaac ccctctgctc gaaatgg
//
LOCUS       DASQQQ010000199         8619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_153591, whole genome shotgun sequence.
ACCESSION   DASQQQ010000199 DASQQQ010000000
VERSION     DASQQQ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8619
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153591"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VE646_07620"
     CDS             <1..622
                     /locus_tag="VE646_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HZD79892.1"
                     /translation="DLTCTETVRLSDDRRLTAIQLQWEYLERAGKYVEQEDDHPANRE
                     VLRRWEQTLTALEAEPLSLHRELDWVAKYRLLEGYRERDGLAWSDPKLRLIDLQYHDV
                     RQDKGLYHRLARAGKVERIASDEEIERAVMEPPSDTRAFFRGRCITKYPQAIAAASWD
                     SMIFDTGRDALQRIPMREPLRGTSAHVGDLLERSPDAATLVDLLRS"
     gene            741..950
                     /locus_tag="VE646_07625"
     CDS             741..950
                     /locus_tag="VE646_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HZD79893.1"
                     /translation="MPQEQEQKRTQRTTGGGAGEDASDAGQAQVTSRGEELKEEMDDI
                     LDEIDSVLEENAEEFIKSYVQKGGE"
     gene            947..1819
                     /gene="prcB"
                     /locus_tag="VE646_07630"
     CDS             947..1819
                     /gene="prcB"
                     /locus_tag="VE646_07630"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043806103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HZD79894.1"
                     /translation="MRDQPLAPPVASGGPIGADPSFFHLVVREHPELARPMTITASPE
                     HLGPHGTTVLGMKFGGGVVLAGDRRATEGYAIAEDKMEKVFAADDLSVIAIAGAAGQA
                     VEIVKLFQLELEHYEKITGDRLSLEGKANRLAQMIRGNFPLAVQGLVVVPLFGGYDER
                     RSEGRIFYYDATGGRWEEEDYHSTGSGGRPAKSSMKKRWRPQMPFEEAVRVAVEALLD
                     AAEEDVATGGPDPARGIFPTVLAVTAEGVRQVSQEDLRAAYEAVVAQRSRDRGSQTSQ
                     TGTGTGTGTGAEAS"
     gene            1819..2505
                     /gene="prcA"
                     /locus_tag="VE646_07635"
     CDS             1819..2505
                     /gene="prcA"
                     /locus_tag="VE646_07635"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016905360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HZD79895.1"
                     /translation="MSFMPYVPPEQLMKDRSEYAHKGIARGKSVVGIEYADGLLFLAE
                     NPSTTLHKISELYDRIAFAGVGKYSEFEDLRIAGVRLADLRGYSYGREDVKAKDIANA
                     YSQALSTIFTQQMKPYEVEILVGEVAGDHGNNELYHILFDGSVTDEHGFVGIGGHADA
                     LSSALQGSYHEDWDLATAVREAVTALSQAEGGQVEPASIEAGVLDRTRGARRKFRRLP
                     ADQIEQILAG"
     gene            2543..3901
                     /gene="pafA"
                     /locus_tag="VE646_07640"
     CDS             2543..3901
                     /gene="pafA"
                     /locus_tag="VE646_07640"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HZD79896.1"
                     /translation="MDRRIFGLENEYGVTCTFRGQRRLSPDEVARYLFRRVVHWGRSS
                     NVFLENGARLYLDVGSHPEYASPECDSLTDLVAHDKAGERILEALLAAAEVRLHEEGI
                     SGQVYLFKNNTDSAGNSYGCHENYLVARQGEFARMADILIPFFVTRQIYCGAGKVLHG
                     PRGAQFCIAQRAEHIWEGVSSATTRSRPIINTRDEPHADAERFRRLHVIVGDSNMSEW
                     TTWVKVGITDLVLRMVEANTVMRDLTLENPIRAIREISHDVACRRKVRLANGRELTAI
                     EIQDEYYQRTARFTERRGADAQTTRLLQEWRGALDALAAGAPDRLSRKIDWVAKQRMI
                     ERYRSKHQLPLSSPKVALLDLAYHDVNRDRGLYYLLERSGKMERVVGEREIARAMREP
                     PQTTRAKLRGDFIKQAKQKRRDYTVDWVHLKLNDQAQRTVLCKDPFRSVDDRVEQLIA
                     HM"
     gene            complement(3912..5012)
                     /locus_tag="VE646_07645"
     CDS             complement(3912..5012)
                     /locus_tag="VE646_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013152537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HZD79897.1"
                     /translation="MCADRASVRLRRGTVLAVRPTPSTALELEVEVDGRPAAALAYPE
                     LTGPVDVGDVVVVNTTARWLDLGTGGMDLVMAVEGAAPTELHHAGRVMKARYTPSQAA
                     VLSVEEVHRDVLESSAGLRSTPVVGAPLHSMLGPIAAGAKAAAGARVVYLMTDGAALP
                     GAFSRLLPRLREAGLVDGFITCGQAFGGDLEAVSVWTGLLAAVEVLGAEVVVAADGPG
                     NLGTDTKWGVSALGSGNLLNAAQAVGGRPVAALRMSFTDPRERHRVVSHHSLTILGDV
                     CRVPANVAVPVIDEEATRTQVWDVLRSRKLEDRHQLIEVDGRPGVELLSRLGVEVRSM
                     GRSAGDDPIFFLAASAAGVLAGRMAANARRYR"
     gene            5036..5998
                     /locus_tag="VE646_07650"
     CDS             5036..5998
                     /locus_tag="VE646_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HZD79898.1"
                     /translation="MHPLERLVDLVALLLESPRPATFDRIRETMPEAYGQEDVSSAKR
                     MFERDKDILRDIGVPIEVTATDVWEVEQGYTISKERYYLPEVDFSPEEISALFVAART
                     GSQDAFAEQAVRKLLSGTEGGLLTGASPAPLAAETGSSDARLLRAAEAIAERRVVTFS
                     YRTARGETSERTVDPWGLVFRGGHWYLVGMDRQRGEIRAFRLSRLTGELSVGDPAEAP
                     PEGFRAADQVQVGPWGPGEPGEPGERARIAFSPDVSWWAVRGLQGVREERNRSDGWVE
                     FSLPAAPGDGLVSWALSFGPDAELLEPVRLRHEVTRRLEAVLAG"
     gene            5988..6986
                     /locus_tag="VE646_07655"
     CDS             5988..6986
                     /locus_tag="VE646_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HZD79899.1"
                     /translation="MPAEGERRKRVSRASERLERLLAVVPYLVRHPGTELVELGRLFG
                     VGEDELLEDLNRLFMSGLPPYGPGDLIGVEIDEGRVWIDMADYFSRPLRLTRVEALAL
                     YLRGTELAATPGLPESAPLSSALGKLRGSLGPDGLGDLGGRVEWTRSDGPDDTLQTLR
                     RAAEGRERLSIEYYAASSAERTEREIDPEEVFFAIGNWYVAAFDHRSGQERLFRADRI
                     RTVARTGVSFEPRGLEGAGRPLYTPTDRDLWIRLRLRPEARWVAEYYEVEDQRERGDG
                     DLEVVLPSARLEWVARLVLRLSGQAVVLDPPELKDRVRELAERTRKRYERGSPHDV"
     gene            7075..7419
                     /locus_tag="VE646_07660"
     CDS             7075..7419
                     /locus_tag="VE646_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79900.1"
                     /translation="MGPESILLSVTRGGREGTYRFTCPTCLDAVEKRADRKIVALLVS
                     AGVDVASQADGTGRLGLDTESTFDRGDPMEDVDPRGKLPQGPAFTIDDLIDFHFRLAD
                     DRYIQDFFAQQH"
     gene            7448..7672
                     /locus_tag="VE646_07665"
     CDS             7448..7672
                     /locus_tag="VE646_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79901.1"
                     /translation="MSAPAIVWIVVGSTTTVAMAAAIIALARHVLVLGRSLRRFQEEI
                     QPLTEDIVDGATRASSRGQRLRSELPSGPR"
     gene            7724..8215
                     /locus_tag="VE646_07670"
     CDS             7724..8215
                     /locus_tag="VE646_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZD79902.1"
                     /translation="MFNIGPLELMVILVIALLVIGPKRLPDVGRSVGRALREFRRAQD
                     EVTRTITGGLDEEQDIESGEAVPGAQATGTSTGPEGSGPAPVDPTTDAVRTLGRSLAE
                     LRRAREELQRGFRVDLNELTRPAPPPRRTATTRPTPPPQPVTEETPPGPDVVPGQAAG
                     TQE"
     gene            8291..>8619
                     /locus_tag="VE646_07675"
     CDS             8291..>8619
                     /locus_tag="VE646_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZD79903.1"
                     /translation="MTLVEHLEELRTRLFIALASIGVASIVGWFLYNPVLRLLRDPFC
                     KSLDNLPVGSRPPTGCEFVFIGAVDAVVIKLKVVVFLGLLIALPVVLYELWRFIVPGL
                     TKRERRMA"
BASE COUNT         1337 a         2986 c         3093 g         1203 t
ORIGIN      
        1 agacctcacc tgcaccgaga ccgtgcggct ctcggacgat cggcggctca ccgcgatcca
       61 gctccagtgg gagtacctgg agcgggccgg gaagtacgtg gagcaggagg acgaccaccc
      121 ggcgaaccgg gaggtgcttc ggcggtggga gcagaccctc accgcgctcg aggccgaacc
      181 gctgtccctg caccgcgagc tggactgggt ggccaagtac cggctcctgg agggctaccg
      241 cgagcgtgac gggctcgcct ggtccgatcc caagctccgc ctgatcgacc ttcagtacca
      301 cgacgtccgg caggacaaag gcctctacca tcgcctggcc agggcgggga aggtggagcg
      361 gatcgcctcg gacgaggaga tcgagcgggc cgtgatggag cccccctccg acacacgcgc
      421 cttcttccga ggccggtgca tcacgaagta cccacaggcc atcgcggcgg cgtcgtggga
      481 ctccatgatc ttcgacaccg gccgcgatgc cctgcagcgc atccccatgc gcgaacccct
      541 ccgcgggacc tcggcgcacg tgggggatct gctcgagcgc tcgcccgatg cggcgacgct
      601 cgtggacctg ttgcgctcgt gaggccgacg gcgccgacat ccggcgcgga gcgcaggtcc
      661 gggtcgaccg gggtggtcct gggctcccga tagaatcgaa gggacaggag cgggggcagg
      721 agcggaagga ggctcgagcc gtgccgcagg agcaggagca gaagcggacc cagcgcacga
      781 ccggtggtgg cgccggcgag gatgcgtccg atgccggaca ggcgcaggtg acctcccggg
      841 gcgaagagct gaaggaggag atggacgaca tcctcgacga gatcgactcg gtgctcgagg
      901 agaacgccga ggagttcatc aagagctacg tccagaaggg cggcgaatga gggaccagcc
      961 gctcgcgccg ccggtcgcgt ccggtggccc catcggtgcg gacccgagct tcttccacct
     1021 cgtggtgcgg gagcatcccg agctcgcccg gcccatgacc atcacggcct ccccggagca
     1081 cctcggcccg cacggcacca cggtcctcgg catgaagttc ggcggcggcg tggtcctggc
     1141 cggtgaccgc cgcgccaccg aggggtacgc catcgccgag gacaagatgg agaaggtctt
     1201 cgctgccgac gatctctcgg tgatcgccat cgccggcgca gcgggccagg ccgtcgagat
     1261 cgtgaagctg ttccagctgg agctcgagca ctacgagaag atcaccggcg atcggctctc
     1321 gttggagggc aaggccaacc ggctggccca gatgatccgg gggaatttcc ccctggcggt
     1381 ccagggactg gtggtggtcc cgctgttcgg gggctacgac gaacgccgct cggagggtcg
     1441 gatcttctac tacgacgcca ccggtggacg gtgggaggag gaggactacc actccacggg
     1501 ctcgggaggt cgaccggcga agagctccat gaagaagcgt tggcggcccc agatgccctt
     1561 cgaggaggcg gtccgggtgg cggtggaggc cttgctggat gccgccgagg aggacgtggc
     1621 caccggtggc ccggatcccg cgcgtggcat cttccccacg gtgctcgcgg tcaccgccga
     1681 gggcgtgcgc caggtgtccc aggaggacct gcgcgccgcc tacgaggcgg tggtggccca
     1741 gcggagccgg gatcggggat cccagacctc gcagaccggc accggcaccg gcacgggcac
     1801 cggcgcggag gcatcctgat gtcgttcatg ccgtacgtcc cgcccgagca gctcatgaag
     1861 gatcgctccg agtacgctca caagggcatc gccagaggca agtcggtggt cggcatcgag
     1921 tacgcggacg ggctgctgtt cctggccgag aaccccagca ccacgctcca caagatctcc
     1981 gagctctacg accgtatcgc cttcgccggg gtgggcaagt acagcgagtt cgaggacctg
     2041 cggatcgcgg gggtccggct ggcggacctg cgcgggtact cctacggacg cgaggacgtg
     2101 aaggccaagg acatcgccaa cgcgtactcc caggccctgt ccacgatctt cacccagcag
     2161 atgaagccgt acgaggtgga gatcctggtc ggggaggtgg ccggggacca cggcaacaac
     2221 gagctgtacc acatcttgtt cgacggctcg gtcaccgacg agcacggctt cgtcggcatc
     2281 ggcggacacg ccgacgccct gtccagcgcg ctccagggct cgtaccacga ggactgggat
     2341 ctggccacgg cggtgcggga ggcggtgacg gcgctctcgc aggcggaggg cgggcaggtc
     2401 gagccggcct cgatcgaggc cggcgtgctc gaccgaaccc gcggggctcg acgcaagttc
     2461 cgtcgcctgc ccgcggacca gatcgagcag atcctggcgg gctgaccctc tgcgatcggc
     2521 tggtcggacc tacactcctc gtgtggaccg acggatcttc ggcttggaga acgagtacgg
     2581 cgtcacctgc acgttccgcg gccagcgacg gttatcaccc gacgaggtcg cccgctacct
     2641 gttccggcgg gtggtgcact ggggacggtc ctcgaacgtg ttcctggaga acggcgcccg
     2701 actgtacctg gacgtcggct cgcatcccga gtacgcgagc cccgagtgcg attccctcac
     2761 cgacctggtg gcccacgaca aggcggggga gcggatcctg gaagccctcc ttgccgcggc
     2821 cgaggtccgg ctccacgagg aaggcatcag cgggcaggtc tacctgttca agaacaacac
     2881 cgactcggcc gggaactcgt acggctgcca cgagaactat ctggtggccc gacagggcga
     2941 gttcgcccgg atggcggaca tcctgatccc cttcttcgtg acccgccaga tctactgtgg
     3001 ggccggcaag gtgctccacg gcccccgggg cgcgcagttc tgcatcgcgc agcgggccga
     3061 gcacatctgg gagggcgtct ccagcgccac cacccgctcc cggcccatca tcaacacgcg
     3121 cgacgagccg cacgccgatg cggagcgctt ccgtcgcctc cacgtgatcg tgggcgactc
     3181 gaacatgagc gagtggacca cctgggtgaa ggtgggcatc accgacctgg tgctgcggat
     3241 ggtcgaggcg aacaccgtca tgcgcgacct caccctggag aacccgatcc gagcgatccg
     3301 cgagatctcg cacgacgtgg cgtgccgccg gaaggtgcgt ctggccaacg gtcgggagct
     3361 gaccgcgatc gagatccagg acgagtacta ccagcgcacg gcgcgcttca ccgagcgtcg
     3421 gggcgccgat gcccagacca cgcggctgct ccaggagtgg cggggggcgc tcgacgcgct
     3481 ggcggccggg gcgcccgacc ggctgtcccg gaagatcgac tgggtggcca agcagcggat
     3541 gatcgagcgc taccgcagca agcaccagct cccgctgtcc tcgccgaagg tggccctgct
     3601 ggacctggcc taccatgacg tgaaccggga ccggggtctg tactacctgc tggagcgctc
     3661 gggcaagatg gagcgggtgg tgggggagcg cgagatcgcc cgggccatgc gggaaccgcc
     3721 gcagaccacg cgggccaagc tgcggggtga cttcatcaag caggcgaagc agaagcggcg
     3781 ggactacacg gtggactggg tccacctgaa gctgaacgac caggcccagc gcaccgtgct
     3841 gtgcaaggac cccttccgga gcgtggacga ccgcgtggag cagctgatcg cacacatgtg
     3901 agccgccggg ctcagcggta ccgtcgggcg ttcgccgcca tccggccggc cagcaccccg
     3961 gccgcagagg cggccaggaa gaagatcgga tcgtcccccg cgctccgacc catggatcgg
     4021 acctccaccc cgagccggga cagcagctcc acgccgggac gtccatccac ctcgatcagc
     4081 tgatggcgat cctccagctt gcgggaccgg aggacgtccc acacctgcgt gcgggtggcc
     4141 tcctcatcga tcacgggcac cgcgacgttg gccgggaccc ggcacacgtc gcccaggatc
     4201 gtgagcgagt ggtgggagac gacgcggtgt cgctcccggg gatcggtgaa ggacatccgc
     4261 agggcggcca ccggacgacc ccccaccgcc tgggccgcgt tgagcaggtt gccgctcccc
     4321 agggccgaga ccccccactt cgtgtcggtg cccaggtttc cgggaccgtc ggccgccacc
     4381 accacctccg cgcccagcac ctccaccgcg gccaggagac cggtccagac cgagaccgcc
     4441 tccaggtccc cgccgaacgc ctggccgcac gtgatgaacc cgtccaccag accggcctca
     4501 cgaagacggg gcaggagtcg cgagaacgcg ccggggaggg cggccccgtc ggtcatcagg
     4561 tagacgaccc tggccccggc ggcggccttc gcgccggcgg cgatgggtcc gagcatcgaa
     4621 tgcagcggcg cccccaccac cggcgtgctg cgcaagccgg ccgaggactc gaggacgtcc
     4681 cgatggacct cctcgaccga gagcaccgcg gcctgcgacg gtgtgtaccg agccttcatc
     4741 acccgtccgg catggtgcag ctcggtggga gcggctccct ccacggccat caccaggtcc
     4801 atgcccccgg tccccaggtc cagccaccgg gccgtggtgt tcaccaccac cacgtcgccc
     4861 acgtccaccg gcccggtcag ctccgggtac gcgagggccg cggccggccg gccgtcgacc
     4921 tccacctcga gctccagggc ggtggagggg gtgggacgca cggcgagcac ggtgccccgg
     4981 cggagcctga cggatgcccg gtcggcgcac atggcccgag tatgatgccg cacgcatgca
     5041 cccgctcgag cgtctggtcg acctggtcgc gctcctgctg gagtcaccgc gcccggcgac
     5101 cttcgaccgg atccgggaga cgatgcccga agcgtacggg caggaggacg tcagcagcgc
     5161 caagcgcatg ttcgagcggg acaaggacat cctgcgcgat atcggcgtcc cgatcgaggt
     5221 cacggccacc gacgtgtggg aggtcgagca gggctacacg atctcgaagg agcggtacta
     5281 cctacccgag gtggacttct cgccggagga gatctcggcg ctgttcgtgg ccgcccgtac
     5341 cgggagccag gacgccttcg ccgagcaggc cgtgcgcaag ctgctctcgg ggaccgaggg
     5401 cggtctgctt accggcgcga gcccggcccc cctggccgcc gagaccggct cctctgacgc
     5461 tcgcctgctg cgggcggcag aggccatcgc cgagcgtcgg gtcgtgacct tctcgtaccg
     5521 aaccgcccgc ggtgagacct ccgagcgcac cgtcgatccc tggggcctcg tcttccgcgg
     5581 cgggcactgg tacctggtgg ggatggatcg ccagcggggg gagatccgcg cgttccgcct
     5641 ctccaggctg accggcgagc tgtccgtggg cgaccccgcc gaagcaccgc cggagggctt
     5701 ccgggccgcc gaccaggtgc aggtggggcc gtgggggccg ggggagccgg gggagccggg
     5761 ggagcgagcc aggatcgcgt tctcccccga cgtgtcctgg tgggcggtcc gggggctgca
     5821 gggcgtgcgc gaggagcgga accggtccga cggatgggtc gagttcagcc tgccggccgc
     5881 ccccggggac gggctggtct cgtgggccct ctcgttcggt ccggacgccg agctgctcga
     5941 gccggtccgg ctgcgccacg aggtgacccg acgtctggag gcggtccttg ccggctgagg
     6001 gtgagcgccg caagcgggtc tcgagggcct ccgagcgcct ggagcgcctg ttggccgtgg
     6061 tcccgtacct ggtccgccac ccggggaccg agctggtcga gctcggcagg ctcttcggcg
     6121 tgggggagga cgagctgctg gaggacctga accggctgtt catgtcggga ctgcctccct
     6181 acggcccggg ggacctgatc ggcgtcgaga tcgacgaggg tcgcgtgtgg atcgacatgg
     6241 ccgactactt ctcccgcccc ctccggctga cccgggtgga agcccttgcc ctgtacctgc
     6301 gggggaccga gctggccgcg acgcccgggc tcccggagtc ggcaccgctc tcctccgccc
     6361 tgggcaagct ccgaggatca ctcggcccgg acggactcgg ggacctgggc ggtcgcgtcg
     6421 agtggacccg gtccgatggt ccggacgaca cgctgcagac cctccgtcgg gcggcggagg
     6481 gccgggagcg tctctcgatc gagtactacg cggcctccag tgccgagcga accgagcgcg
     6541 agatcgaccc ggaggaggtc ttcttcgcga tcggcaactg gtacgtggcg gcgttcgacc
     6601 atcggtcggg acaggagcgg ctgttccgag ccgatcggat ccgcaccgtc gctcgcacgg
     6661 gggtgagctt cgagccccga gggctcgagg gtgccgggcg gcccctgtac acccccaccg
     6721 accgggacct gtggatccgg ctgcggttgc gccccgaggc tcgctgggtc gccgagtact
     6781 acgaggtgga ggaccagcgc gaacgggggg acggggatct ggaggtcgtg ctgccatcgg
     6841 cccgtctgga gtgggtggcc aggctggtgc tgcgcctttc gggtcaggct gtcgtcctcg
     6901 atccgccgga gctgaaggac cgggtccgag agctcgccga gcggacccga aaacggtacg
     6961 aacgaggtag tccgcacgac gtatagccgg ctaaaccgct cctcgggcgc cgccgataca
     7021 ggtcgtgtta tgacgaccat ccggacgaca tgcccccggt gcggggaagt ggacatgggc
     7081 cccgagtcga tcctgctatc ggtgacgcgg ggcggccggg agggcaccta ccgcttcacc
     7141 tgccccacct gtctcgacgc cgtggagaag cgcgccgacc gcaagatcgt ggccctgctg
     7201 gtctccgcgg gtgtggacgt cgccagccag gccgacggca ccggacggct cggtctggac
     7261 accgagtcga cgttcgaccg gggggacccc atggaggatg tggaccccag gggcaagctg
     7321 ccccagggcc ccgccttcac gatcgacgat ctgatcgact tccacttcag gctcgccgac
     7381 gaccggtaca tccaggactt cttcgcccag cagcactgag cccctcggtc cggtagcatc
     7441 ctcgtcggtg tcagctcccg ccatcgtgtg gatcgtggtg ggctccacca ccaccgtggc
     7501 catggccgcc gccatcatcg cgctggcccg ccacgtgctg gtgctgggcc ggagcctgcg
     7561 gcggttccag gaggagatcc agccgctgac cgaggacatc gtggatggag cgacgcgggc
     7621 ctcgtcccgc ggccagcgac tgaggtccga actcccgtcc ggtcccaggt agggcctccg
     7681 cagtagaatc gacaggagcc cctcccgacg tacggagcag cccgtgttca acatcggacc
     7741 cctcgagctc atggtgatcc tggtgatcgc gctcctggtg atcgggccga agcgcctccc
     7801 cgacgtgggg cgatccgtgg ggcgggcgct ccgggagttc cgccgggcgc aggacgaggt
     7861 gacgaggacc atcaccggcg gcctcgacga ggagcaggac atcgagagcg gcgaggcggt
     7921 cccgggtgcc caggccacgg gcacctcgac gggtcccgag gggtccggcc cggcgccggt
     7981 ggatcccacc accgacgccg tgcggacgct cggccggagc ctggccgagc tgcgccgggc
     8041 tcgggaggag ctccagcggg gattccgcgt cgacctgaac gagctgaccc ggcccgcccc
     8101 tccgccccgc cggaccgcaa ccacccgtcc gacgcccccg cctcagccgg tcaccgaaga
     8161 gacaccgccc gggccggacg tggtcccggg ccaggccgcc gggacgcagg agtagtcccg
     8221 ggcgggccgg tcgatggcgt ccatcacgaa gcgattccgc cgcccgggcc gccgtgccgc
     8281 cgatggctcg atgaccctgg tcgagcacct cgaggagctc cggaccaggc tcttcatcgc
     8341 tctggcctcg atcggggtgg cctcgatcgt cgggtggttc ctgtacaacc cggtcctgcg
     8401 cctgctgcgt gaccccttct gcaagagcct ggacaacctc cccgtcggct cccggccccc
     8461 gaccggatgc gaattcgtat tcatcggggc ggtggacgcg gtcgtcatca agctgaaggt
     8521 cgtggtgttc ctcggtctgc tgatcgccct gccggtggtc ctgtacgagc tctggcggtt
     8581 catcgtcccc ggtctcacca agcgcgagcg ccgcatggc
//
LOCUS       DASQQQ010000200         4309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_210634, whole genome shotgun sequence.
ACCESSION   DASQQQ010000200 DASQQQ010000000
VERSION     DASQQQ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210634"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2076)
                     /locus_tag="VE646_07680"
     CDS             complement(<1..2076)
                     /locus_tag="VE646_07680"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZD79904.1"
                     /translation="MTTSIFGSVVHRTEDPRFLEGRSRYVDDISIDGALRAVFVRSMM
                     AHARLGGIDPRDALAMPGVVAVLTAADLDLRPQPPSGNVHGPFDRPVLASGVVRFVGE
                     PLAVVVAEDLARAEDAAEVVAVDYDPLPPLVDPERALDPTAPLLFAAAGTNLAHEFAR
                     GWDEDVLAGAEVVVRGRFVNQRLAPVPMETNACAVVPEPDDRLTVWVSTQIPFDVRSD
                     LAEWLGLDKARIRVIAPDVGGGFGAKLITYPEYLVVAAAAIRLGRPVRWAETRTENLV
                     SMTHGRAQIQRVELGARRDGTLVGLRADLLADLGAYPLAGYLAPTTRSMLCGVYRIPK
                     VASRGRSVVTNTTPVSAYRGAGRPEATALIERAIDLLAAELGMDPVELRRRNLIPPEA
                     FPYRTSVGTTYDVGDYPGALDAVLRLADQEGLRAEQAERRSRGDRVQLGVGISTYVEV
                     TAFAGREFGSLEVHPDGRVTVLTGVSPHGQGHETSLAQIASGLLGIPMERITVIHSDT
                     GVVPAGDGTYGSRSLQLGGTAVWNAAERVVEKARVLAAHLLEASPADVTVHEHGRIGV
                     TGAPEASLGWGELATVAADPGRLPEGMEPGLSAGGKFHQKDSTYPFGAHLAVVEVDIE
                     TGEARLVRHVAVDDCGRILNPLLVDGQVHGGLAQGVAQALYEGVAYDELGSPLSGNLT
                     GYLVPSAAEL"
     gene            2444..3031
                     /locus_tag="VE646_07685"
     CDS             2444..3031
                     /locus_tag="VE646_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79905.1"
                     /translation="MLRTGIDPSIERITEPIVAWRAWTLFSRGHHAELRLRPIVGGRR
                     SWPPRRPAGASCPRRCRPVPGVDCVCGLYATHDPLALRHARDPAVVGTVALWGRVVEH
                     DLGYRASRAYPQRLALVCHLCFWQWGLDRARPDHVARLRGGRLVPLCRPHVEMSNRYG
                     YRTPSLMPADAVEQALLDTYAVDPLRVEPGDPSGI"
     gene            complement(3119..3976)
                     /locus_tag="VE646_07690"
     CDS             complement(3119..3976)
                     /locus_tag="VE646_07690"
                     /inference="COORDINATES: protein motif:HMM:NF038012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZD79906.1"
                     /translation="MFIAIVAAVAAGVSFAVGGVVQQRVARTRPEGALSPGLLLSLAR
                     VPLWWAGIGFALLSYGLQSLALAFGPLVLVQPLLVTEVIFAIPISVRIHGMHLELREW
                     IGVSCVVAGLALGIVSARPTSGNPLPALVLWGLAVAASGFVILGALGAARPAEGPVRA
                     SFMAAAAAVALALTSALLKSMVAVFQHHGLGALAVWQPYAMAVVAIGGLYLVQSAFRS
                     GPLAASMPVIDGVEPSFAIGLGIALFGERVQSGVWPFLGIAVGLALLFAGIVLLDTSP
                     VIQRLHERE"
     gene            4117..>4309
                     /locus_tag="VE646_07695"
     CDS             4117..>4309
                     /locus_tag="VE646_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZD79907.1"
                     /translation="MVTLVRSPGGGGHVKCWERLTEAAAATPNLVDLTVYFLGPTERT
                     TELGSNARFVTLPPRLGTGR"
BASE COUNT          660 a         1593 c         1507 g          549 t
ORIGIN      
        1 gagctccgcc gcgctgggaa cgaggtaacc ggtgaggttg cccgagagcg gcgagccgag
       61 ctcgtcgtac gcgacaccct cgtacagggc ttgagcgacg ccctgcgcca gacccccgtg
      121 cacctgaccg tccacgagga gtgggttcag gatccggccg cagtcgtcca cggccacgtg
      181 gcgcaccaga cgcgcctcac cggtctcgat atccacctcg accacggcca ggtgcgcccc
      241 gaaggggtag gtggagtcct tctgatggaa cttgcccccc gccgacaacc ccggctccat
      301 gccctcgggc agacgcccgg gatcggccgc cacggtcgcc agctcccccc agccgagcga
      361 ggcctccggt gcgccggtca ccccgatccg gccgtgctcg tgcaccgtca cgtcggccgg
      421 cgacgcttcc agcaggtgcg ccgcgaggac ccgggccttc tccaccacgc gctcggccgc
      481 gttccacacc gcggtcccac cgagctgcag ggaccgcgaa ccgtaggtcc cgtcgcccgc
      541 gggcaccacc cccgtgtccg agtggatcac ggtgatccgc tccatgggga tccccagcag
      601 cccggaggcg atctgcgcga gcgatgtctc atggccctgt ccatgcggcg agaccccggt
      661 gagcacggtg acccggccat cgggatggac ctccagggag ccgaactcgc ggccggcgaa
      721 ggccgtcact tccacgtagg tgctgatccc gacgccgagc tgcacacggt ccccccgcga
      781 ccgtcgctcg gcctgctcgg cgcgaaggcc ctcctggtcc gccagccgaa gcaccgcgtc
      841 caacgcgccg ggatagtcgc ccacgtcgta cgtggtccct accgacgtgc ggtacgggaa
      901 cgcctcgggc gggatcaggt tccgacgccg gagctcgacc gggtccatcc cgagctcggc
      961 ggccagcagg tcgatggccc gttcgatcag cgccgtggcc tccggtcgcc ccgcgccccg
     1021 gtaggcggac accggggtcg tgttcgtcac caccgagcgc ccgcgggagg cgaccttcgg
     1081 gatccgatag acgccgcaca gcatggaccg ggtggtcggc gccaggtagc cggccagggg
     1141 gtacgcgccc aggtccgcca gcaggtcggc ccggagcccc accagcgttc cgtcgcgcct
     1201 ggccccgagc tccacccgtt ggatctgggc tcggccgtgc gtcatgctca ccaggttctc
     1261 ggtccgggtc tcggcccacc gcaccggccg gcccaggcgg atcgcggccg ccgccaccac
     1321 caggtactcg gggtaggtga tgagcttggc cccgaagcca ccccccacat cgggtgcgat
     1381 cacgcggatc ctggccttgt ccagtcccag ccactccgcg aggtcgctgc gtacgtcgaa
     1441 cgggatctgc gtcgagaccc agaccgtgag gcggtcgtcc ggctcgggca ccaccgcaca
     1501 ggcgttcgtc tccatcggca ccggtgccag gcgctggttg acgaagcggc cccgcaccac
     1561 gacctcggcc cctgcgagga cgtcttcgtc ccacccccgc gcgaactcgt gggccaggtt
     1621 cgtcccggcc gcggcgaaga gcaacggggc ggtcgggtcc agggcccgct cgggatccac
     1681 cagcggcggc agcggatcgt agtcgaccgc caccacctcg gcggcgtcct cggcccgtgc
     1741 caggtcctcg gccaccacca cggccagtgg ctcccccacg aaccggacca ccccggacgc
     1801 cagcaccggg cggtcgaagg gaccgtgcac gttgcctgag ggaggctggg ggcgcagatc
     1861 cagatcggcg gcggtcagga cggcgaccac gccgggcatc gccagcgcgt ccctagggtc
     1921 gatgccgccc aggcgcgcat gcgccatcat ggagcgcacg aacacggccc gcagcgcccc
     1981 atcgatgctg atgtcgtcca cgtaccggct tcggccctcg aggaaccgtg ggtcctcggt
     2041 gcggtggacg acgctgccga agatcgaggt ggtcatgcgg cgcccactct agaggaggca
     2101 gaagcagcaa cgccggggaa ggcgcagccg acgggtggcg atgatgaccc tcgccgcgtg
     2161 cctgagcctc atggcgagcc tcgtcggtgg aggcgcgaac agcatcagcg ctcgccaggt
     2221 ccggcagagg gcggggcttt tccggcgcgg aacccgaggg tagggtcagg tatcgggcgg
     2281 atccgtcgtc cccaacaagg gaggtgatgg tgtgaacatc ggcgaaccgc gacgcgtgat
     2341 cgaagtggag ccggtctccc tcccggtgcc cgaggtcctt cccgaccccg aaccggctcc
     2401 ggagaaagag ccagttcgca cgcctgcccc gtagtcggtc ggcatgctgc ggaccgggat
     2461 cgatccgtcc atcgagcgga tcaccgagcc gatcgtggcg tggcgggcgt ggaccctgtt
     2521 ctcgcgaggg catcacgcgg agctccgcct gcgacccatc gtgggcgggc gacgttcctg
     2581 gccgccccgg cgcccggcgg gtgcctcctg tcccaggcgg tgccggccgg tgccgggcgt
     2641 ggactgcgtc tgcggtctct acgccaccca cgacccgctg gccctgcgac acgcccgtga
     2701 ccccgcggtc gtggggaccg tggcgctgtg gggccgcgtg gtcgagcacg acctcgggta
     2761 tcgcgcttcg cgagcctacc cgcagcgact cgcgctcgtg tgccacctct gcttctggca
     2821 atgggggctg gatcgcgccc ggcccgatca tgtcgcgagg ctccggggcg gtcggctcgt
     2881 gcccctgtgc cgccctcacg tggagatgtc gaatcgctac ggctaccgga ccccgagcct
     2941 gatgccggcc gacgccgtgg agcaggccct cctcgatacc tacgcggtcg atccccttcg
     3001 ggtcgagcct ggtgatccat cggggatcta gccccgggcg aacggacacg cgctcgaacg
     3061 tggggaggga ccatctggac gcgatcttgg cccccgagcg gagggccgga cggccgagct
     3121 actcgcgctc gtgcagccgc tggatcaccg gtgacgtgtc caggagcacg atccccgcga
     3181 acaacagggc cagccccacg gcgatgccga ggaacggcca gacgccgctc tgaacccgct
     3241 caccgaacag ggcgatgccg agcccgatgg cgaacgaggg ctcgaccccg tcgatcacgg
     3301 gcatgctggc agccaagggc ccagatcgaa aggcgctctg cacgaggtac agaccaccga
     3361 tcgccacgac cgccatggcg tacggctgcc acacggccag ggcaccgaga ccgtggtgct
     3421 ggaagacggc gaccatggac ttgagcaggg ccgaggtgag cgccagggcc accgccgcgg
     3481 cggcagccat gaacgaggcc cggaccgggc cctccgccgg ccgtgccgcc cccaacgcgc
     3541 cgaggatcac gaaccccgaa gcggccacgg cgagccccca caggaccagc gccggcaggg
     3601 ggttcccgct ggtggggcgc gcgctcacga tccccagcgc gagccccgcc accacgcagg
     3661 agacgccgat ccactcccgc agctcgaggt gcatcccgtg gatccgcacg gagatgggga
     3721 tcgcgaagat cacctcggtg accagcaggg gctgcacgag gacgagcggc ccgaacgcca
     3781 gcgccagcga ctgcagaccg tacgacagga gcgcgaagcc gatccccgcc caccacaagg
     3841 gcacccgggc cagcgagagc agcagtccgg gcgagagcgc gccctccgga cgagtacggg
     3901 ccacgcgctg ctggacgacc cctcccacgg cgaacgacac ccccgccgcc acggcagcga
     3961 cgatggcgat gaacacgcct ggttgttccc gctcgagcgg ctcggcactc gccgccggtc
     4021 ggcaccggtc gtggtcgtga gcatgtcggt ggcgatgccg ggtaagcgtc tcgtatgcga
     4081 gtggggagcg agcgggagcg tccatcggta ggggtcatgg tcacgctcgt caggagcccc
     4141 gggggcggcg gccacgtgaa gtgctgggaa cgactcacgg aagccgcggc cgcgacgccg
     4201 aacctggtcg acctcaccgt gtacttcctg ggccccaccg agcggaccac ggagctgggg
     4261 tcgaacgcgc ggttcgtgac gctgcctccg cggctgggga ccggccggt
//
LOCUS       DASQQQ010000201         3738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_229362, whole genome shotgun sequence.
ACCESSION   DASQQQ010000201 DASQQQ010000000
VERSION     DASQQQ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229362"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..342
                     /locus_tag="VE646_07700"
     CDS             7..342
                     /locus_tag="VE646_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HZD79908.1"
                     /translation="MPYEEDLAERIRELIAAEAGVTEKEMFGGLAFLIGGNMAVAASG
                     EGGIMVRVDPAISDRLVATTRARPVVMRGRPMQGWLRVDTQHLRDRRQLSRWVERGVS
                     FARSLPAKS"
     gene            382..1461
                     /locus_tag="VE646_07705"
     CDS             382..1461
                     /locus_tag="VE646_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two pore domain potassium channel family
                     protein"
                     /protein_id="HZD79909.1"
                     /translation="MRWVALPAGIVVLGWTLLDVFRTLVMPRAARGRFRLSRILFMPM
                     WRPWRWVGTRRKTIQAREHFLSVAAPFFFFVLLITWVLLALVGYALILWTPAFLDPGS
                     PGGASFANALYVSGTSLFTLGFGAAGATGWTRSIEVLAGATGLGMFAVVIAYLPVLYQ
                     AFNRREVGVLLLDARAGSPPSGPELLHRMGSAGMTSSLSELFVEWERWAADVLETHLS
                     YPLLAFFRSPHDYTSWVTALGSVLDAATLIITATEDEPDQRAKLLYGTGVHAVEDLFY
                     YFRVSERPALIQREEFAEVLQDMKDDGYSVRPLDEAFARFTEKRAKYAPRLDGLSVVL
                     AAPPAQWIGDRSFLGARTQHRTPSE"
     gene            complement(1482..2588)
                     /locus_tag="VE646_07710"
     CDS             complement(1482..2588)
                     /locus_tag="VE646_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HZD79910.1"
                     /translation="MSDAHRLSRREARRIAVQAQLLDGERPAGLHPLVRHLTLLQLDP
                     IAAVAPSADLVVWSRLGSSYSPAALRDAIQDRSLIELHAMIRPAEDIALFRADMGDWP
                     GRGELRPWQHEVREWVQVNDACRRDILARLGVSGPLTMRELPDTCAVAWRSTGWTNNR
                     NVEKLLGFMVQRGEVAVAGRKGPERLWDLASRVYPDHCVVPAAEAKRNRDERRLRALG
                     IARATAPESPVEPVDVGEAGEPAIVEGVRGHWRVDPSVLGRPFSGRTALLSPFDRLVH
                     DRKRALQLFDFEYQLEMYKPAAERRWGYYALPVLHRDRLIGKVDATADRGAGVLRVDT
                     IHEDLPFSKAVAAAVRRELNDLARWLDLRLLLAG"
     gene            complement(2585..2896)
                     /locus_tag="VE646_07715"
     CDS             complement(2585..2896)
                     /locus_tag="VE646_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79911.1"
                     /translation="MSVMLHVTGRIETGRWIEFLEAAERWRESRRARGWALPRVFGAL
                     AGEMNTAMLVFEYPDAATFEREDAAESGDPQYAAVASAMPFAGPIEYRLYREALPAVE
                     A"
     gene            complement(2931..3020)
                     /locus_tag="VE646_07720"
     CDS             complement(2931..3020)
                     /locus_tag="VE646_07720"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HZD79912.1"
                     /translation="MWHCHILEHEDNEMMRPYRVGPLQPGQPT"
     gene            complement(3168..3335)
                     /locus_tag="VE646_07725"
     CDS             complement(3168..3335)
                     /locus_tag="VE646_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79913.1"
                     /translation="MEPGKWTKRLWMEPVTENPAVGATEVWELYNATADAHPIHIHEI
                     TSRWWIGRPSS"
BASE COUNT          513 a         1327 c         1295 g          603 t
ORIGIN      
        1 cgagccatgc cctacgagga ggacctggcg gagcggatcc gcgagctgat cgccgccgag
       61 gccggtgtca ccgagaagga gatgttcggc gggctcgcct tcttgatcgg cgggaacatg
      121 gccgtcgctg cgagcggcga aggggggatc atggtgcggg tcgatcctgc gatctcggac
      181 cggctggtcg cgaccacgag ggctcgtccg gtggtcatgc gcgggcgacc gatgcagggt
      241 tggctccggg tggacacgca gcacctgcgc gaccgccgtc agctcagccg gtgggtggag
      301 cgaggtgtct cgttcgctcg ctccttgcca gccaagtcat gagccgtccc ccgcggtcct
      361 cggccccggt aggctgatcg gatgcggtgg gtcgcgctgc cggcgggcat cgtcgtgctg
      421 gggtggaccc tcctggacgt gttccgcacg ctggtgatgc cgcgggcggc tcggggccgg
      481 ttccgtctca gccgcatcct gttcatgccg atgtggcgac cctggcggtg ggtgggcacc
      541 cggcgcaaga ccatccaggc ccgcgaacac ttcctgtccg tcgccgcgcc cttcttcttc
      601 ttcgtgctgc tcatcacctg ggtgctgctc gcgttggtgg gttacgccct gatcctgtgg
      661 accccggcgt tcctggaccc cggctcgccg ggaggtgcct cgttcgcgaa cgccctgtac
      721 gtgagcggta cctcgctgtt caccctgggc ttcggggccg ccggggcgac cgggtggacg
      781 cgctccatcg aggtcctcgc cggcgccacc ggcctgggga tgttcgcggt ggtgatcgcg
      841 tacctgcccg tgctctacca ggccttcaac cgccgagagg tcggggtgct gctgctggac
      901 gcccgggcgg gatcgccacc ctccggtccc gaactcctcc accggatggg gagcgccggc
      961 atgacctcct cgctctcgga gctgttcgtc gagtgggagc ggtgggcggc cgacgtcctg
     1021 gagacgcatc tgtcgtaccc cttgctggcg ttcttccgtt cgccgcacga ctacacgtcg
     1081 tgggtcacgg cgctcggctc cgtcctggat gccgccaccc tgatcatcac ggccaccgag
     1141 gacgagcccg atcagcgcgc caagctcctg tacggcacgg gtgtccatgc cgtggaggac
     1201 ctcttctact acttccgggt cagcgagcgc ccggccctga tccagcgcga ggagttcgcc
     1261 gaggtcctgc aggacatgaa ggacgatggg tactcggttc ggcctctcga tgaggcgttc
     1321 gcccgcttca ccgagaagcg ggccaagtac gccccgcgcc tcgacgggct ctccgtcgtg
     1381 ctggcggcgc cgcccgcgca gtggatcggc gaccgctcct tcctgggagc tcggacccag
     1441 caccgaaccc cgtcggagtg aggatcccga tcggaccggt ctcagccggc cagcaggaga
     1501 cgcaggtcga gccagcgcgc gagatcattc agctcgcggc gcacggcggc ggccacggcc
     1561 ttgctgaacg gcaggtcctc gtggatcgta tcgacccgga gcaccccagc cccgcggtcc
     1621 gcggtggcgt cgaccttgcc gatcagtcgg tcgcggtgca acacgggcaa cgcgtagtag
     1681 ccccatcggc gttcggccgc cggcttgtac atctcgagct gatactcgaa gtcgaacagc
     1741 tgcagggcac gcttgcggtc gtgcaccagc cggtcgaacg gcgagagaag ggcggtgcgg
     1801 ccggagaacg gacgacccag caccgacgga tccacccgcc agtggcccct gacgccctcg
     1861 acgatggcgg gctcacctgc ctcccccaca tccaccggct ccaccggaga ctcgggcgcg
     1921 gtggctcggg cgatgccgag ggcgcgcagc cgtcgctcgt cgcggttgcg cttggcctcc
     1981 gcggccggga cgacgcagtg gtccgggtag acgcgcgacg ccaggtccca caggcgctcg
     2041 ggtcccttcc ggccggccac cgcgacctcg ccccgctgca ccatgaagcc gagcagcttc
     2101 tccacgttgc ggttgttggt ccagccggtg gatctccagg ctaccgcgca cgtatccggc
     2161 agctcgcgca tcgtcagcgg ccctgagacg ccaagccggg ccaggatgtc ccgccggcag
     2221 gcgtcgttga cctggaccca ctcgcgcacc tcgtgctgcc atggtcgcag ctccccccga
     2281 ccgggccagt cccccatgtc ggcccgaaag agggcgatgt cttcggcggg ccggatcatc
     2341 gcatgcagct cgatcaggct cctgtcctgg atcgcgtcgc gcagcgcggc tggggagtac
     2401 gaggagccca ggcggctcca cacgaccagg tcggcgctgg gtgccaccgc ggcgatgggg
     2461 tcaagctgca gcagggtgag gtggcgaacg agggggtgca gaccggccgg tcgctccccg
     2521 tcgagcagct gagcctgcac agcgatccgg cgcgcctcgc ggcgggagag tcggtgggcg
     2581 tcgctcacgc ctcgaccgcc gggagggctt cccggtacag ccggtactcg atcggaccgg
     2641 cgaagggcat cgccgaggcc acggccgcgt actgggggtc acccgactcc gccgcatcct
     2701 ctcgctcgaa cgtggcggcg tccgggtact cgaacaccag catcgccgtg ttcatctccc
     2761 cggccagggc gccgaacacc ctgggcagcg cccacccgcg ggcccgccgg gactcccgcc
     2821 agcgctcggc agcctccaag aactcgatcc accgaccggt ctcgatgcgg ccggtcacat
     2881 gcagcatgac gctcacgttc ctcaactcct cgaaggggcg accggacgac ctatgtgggc
     2941 tgaccgggct gcaacggacc cacccggtag ggtcgcatca tctcgttgtc ctcgtgctcc
     3001 aggatgtggc agtgccacac gaactgaccc ggcgtccgaa acctggcccg gaccccgcgt
     3061 gatctggccc gggtaggcga tcaccgtgtc cttgaacccg gattcccagg actggggcgg
     3121 ctgcggctcg gattccggca cgacctgcac ttcccgcgtc tgctcgttca cgacgatggc
     3181 ctgccgatcc accacctcga ggtgatctcg tggatgtgga tcgggtgggc atccgccgtc
     3241 gcgttgtaca actcccacac ctcggtggcg cccaccgccg ggttctccgt aaccggctcc
     3301 atccacagcc gcttggtcca cttccccggc tccacgttcg gatcgccggc cagcgtgccg
     3361 agcagcgtct tggccggggc gtcctcgaac gactccgggg ctcgttgaac tccgaggctc
     3421 ctcgaggagc gccaggggcc gcgtcaccgt ctcgctcggg agcgcagcga tcggcggcag
     3481 ctgcaggaac cgtggggggg tggtgggatc cgcggtccgt gaggggacga cacggaacgc
     3541 catcacctga cccgtggtgg ctgggtcggc caccgagaag tcctcgccag gtgtgccgcc
     3601 gccgtacggc tcatccggtc ccacgttggc caggacatga tcgcccaccg ggacgtgggt
     3661 gaagtcgacg atcaggtccg cgcgctcggc gggccccatc agcagtcggt tgccgttggt
     3721 gtccgtcagg ttcacggg
//
LOCUS       DASQQQ010000202         9554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_370108, whole genome shotgun sequence.
ACCESSION   DASQQQ010000202 DASQQQ010000000
VERSION     DASQQQ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370108"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VE646_07730"
     CDS             complement(<1..950)
                     /locus_tag="VE646_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HZD79914.1"
                     /translation="MQEPGAEPPSTPGTSEPGRNRAFSGARARPSGRRAAVSVAESRE
                     GEAPGAIEIQNSSTQRRWATPARRSTPSARASTALASTADGSGPTQGLPRPATQTDQG
                     PGDRRRGDRGVAGAHVQRQEPGPAPRQGPGREREPLPGIDPVLQGQAGHGRIGGRHPV
                     GDHLGAQDPLDPTGHRGHGRGERGPEGRIRHRQPAGPATAALGHETDVPPGPVGRPHA
                     HLLTRARERRGRSWGFLPPGPGPGQWAARGFSSIGRSGRRAPPLPLPAIPTPRPYDAR
                     MLDARDLEIVAALQEDARATYAEVGRKVGLSPSSVHDRVR"
     gene            977..2488
                     /locus_tag="VE646_07735"
     CDS             977..2488
                     /locus_tag="VE646_07735"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZD79915.1"
                     /translation="MRTLYRASTVHTLAHPPVGEWVLVDGRHVERVGSGDPPSADRVV
                     DLPGTAVLPGFVDAHVHLTGTGEHHRVPEVSRARSAQELLETLTRLTQQEQGPLLVHG
                     FDESVWERPEPPSIEALDRITDRPLAVVRVDGHLSLANRGAIQAAGAGDEPGAERDAG
                     GAPTGRLTGAANARLRRWFQQHLSDRDVEQLQLEAASLAVRHGITAVHEMSMPAERGI
                     RDLQILLGHRHRLPVDVVPYVATTDIAQVMDLGLPGIGGDLPVDGSLGARTAWLNEPY
                     RAGEGRGTRAFDDEELAGFFHAGHLAGLQVGVHAIGDAAIEQVVGTWERVYRTLDSRQ
                     RRHFRARRHRIEHFEMAGPDLVERAAALGLAISVQPGFDAAWGHPGSLYEQRLGPNRA
                     RDMNPFRWVLERGSEMGAGSDSPITSIDPLVGIAAFESHHDPSQRLSRAEAIRIYTSG
                     SARLAHLEDKKGSLAPGMHADFSVWDDDPVTAPTLDELGPILTVSLGREVYAS"
     gene            2851..3189
                     /locus_tag="VE646_07740"
     CDS             2851..3189
                     /locus_tag="VE646_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 family protein"
                     /protein_id="HZD79916.1"
                     /translation="MEDIEEQIEDEQLDEPGEPGEIDADASTPPAADDGDGEDVESIE
                     DLLVKKEAQEAEEEDDSVLNMSREERLEPLAVRVVPPQPTEFICKSCYLVKHRSQLKD
                     KARMLCRDCA"
     gene            3111..5453
                     /gene="lnt"
                     /locus_tag="VE646_07745"
     CDS             3111..5453
                     /gene="lnt"
                     /locus_tag="VE646_07745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HZD79917.1"
                     /translation="MQELLSGEAPQPAQGQGAHALPGLRLNRPALRLTLAILISAGCG
                     ILLSLALPPVDAGWIAFLAPAPFLWVIRRSRPLRGLLLGFVFGLSSFGAILWWIMQFG
                     QLAWGALVVVLAASVGVFGLLAPVLWRPSRPVVSSLGLASAWTVTEWIRGMWPVGGFT
                     WGQLGSTQTGNPFLLPLASVGGVWAISFVVLLVAALLVEAGTRVRSRPVGAAGLVAIA
                     AVVVLAPVAIQIPRPTGRILDVATIQVDVRRAAGLPPLREDQRVAELVMRLDRTLTAD
                     PPDLAIWGESSLDPGANLPSFRPRVRSTIRQVGVPTLAGAVVETPSGRFYNQSLLFDG
                     SGRVVGEYRKVHLVPYGEYVPFRRELSWISALRQIPYDVTPGDRVHPLHVDGITFGNV
                     ICFENSFPGIDRELVNQGAGFLVVTTNNASYGFSAASRQHVIMSQLRAVENGRWVVHA
                     AVSGISALIDPQGRIVSETGLFQPAVLRAPVRASTARTLYTRLGDWVPWLALAFTLGL
                     LVAPRRRRHPAREPGPGPARPRSLVLLPTYDERATIAQVIEALLALPQALDVLVIDDA
                     SPDGTAQVVRAMAEGHPRVRLVERPRKAGLASAYLVGFRRALEEGYDLVVEMDSDLSH
                     DPSELPALLAGAARNHLTIGSRYVPGGSVTNWSRSRIALSRAGNRYARLALGLPLSDA
                     TSGYRVFQAALLRHLVQGGIHSDGYGFQIELALRSWNDGFRVGEVPISFRERQHGVSK
                     ISRRIVVEALLMVTLWGLRMRLGRPPVVGPQNAHKGHYGT"
     gene            5713..8469
                     /locus_tag="VE646_07750"
     CDS             5713..8469
                     /locus_tag="VE646_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HZD79918.1"
                     /translation="MRGTRRRPADGRTRRKRWELIGLLLSLTLILSQSIGVVGAAASD
                     GPISEPDDVATATPAVDAPQAAPSPSEAPPAAEDPAPGLGAEPGATTSEQTPSSGGPP
                     AGGDGAGTDQQSPEPSADATKTASRTGGGGGSVAPTLISSPSSGSLNVDLDQFANQPG
                     KGWQNGDLNGNNSAYHEGDVVPFRLAIEGLSAGSHTIHLSYDFTAGGHEAYDFLATWD
                     ASESPSLCASGGGAVSSMCPGLPSAHTHVFPSDPFAPGSPTKAGLTVAGAESFSGLSR
                     NLTMYGGTIDSIGGPAHSGPVSGNSTGDITVHFTSSGSAVLFAWGGHLAESAYWVRTN
                     GDPNGASQVSGAPWHMRTQSLDGSGNKNQDRSIQPSAIVALPALAIQKSADAGTVQIG
                     DQIGYSITVTNTGNVGVSNVSVQDALPGGSGIDWSVAGTTGDTNGLDCSIGGSPPSQA
                     LSCTKDSLAKGGSFTVHVTSPTRGSACGTYDNTASASADGVEVVQSQTATIVVEGCGT
                     DLKITKTASSDAVPYGSSFSYTLTVRNQGSGQATGVVVIDDLADSLTGVSASYDVNPG
                     SPGDTGACSVDTGNLVTCQVATLAPDDGAANGPDTIRIQIQATAPDTCGAITNTAHVS
                     SDQDATGSDSNEVQIDVTGCVTPPAPIGIRIVKGGPALAHVGDTITYTFDVSLTQSTP
                     LSNIAVSDPMCSTAPGLDSKAGGNQDDVLEPGETWRYSCTHVVTSSDPDPLPNTATVT
                     GTSEDERTATDQDSHLVDLIHPAIRIVKTASARSGEPGGSVTYTYVVTNTGDATLFAI
                     SVDDDVIGHICDMASLDPGASSTCTAGYVLPDRSGPIDNVGTAVGSDQTGAKVSDHDR
                     ASVAVVLGTTVTPPPTTTPPKGTAFTGAADAVPLAALALALLLAGSGILFASRRRRQG
                     SAPD"
     gene            complement(8679..>9554)
                     /gene="gcvPB"
                     /locus_tag="VE646_07755"
     CDS             complement(8679..>9554)
                     /gene="gcvPB"
                     /locus_tag="VE646_07755"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HZD79919.1"
                     /translation="RGLVDVGALERLVDEDVAGLMLTNPNTLGLFEEEIERITEVVHA
                     AGGLVYYDGANLNAILGRARPADMGFDIVHINTHKTFATPHGGGGPGAGPVGVVDRLV
                     PYLPGPLVERDADGRFHLQEGGARSIGRMHGFHGNFGVLVRAYAYVFLHGRDGLKEVA
                     DLAVLNANYLAELLGPAFPTAYPGRPMHEFVATGRPLKERTGIRAVDVAKRVIDLGFH
                     PSTVYFPLVVEEAMMVEPTETESTETLDAFAAAMVQAAEEAATDPALLHEAPVSTPVR
                     RLDEARAARHLKLRW"
BASE COUNT         1327 a         3431 c         3454 g         1342 t
ORIGIN      
        1 ttgcggacgc ggtcgtggac cgacgagggc gacagaccca ccttgcggcc cacctcggcg
       61 taggtggccc gggcgtcttc ctggagggcg gccacgatct ccaggtcccg cgcgtccagc
      121 atgcgggcat catacgggcg gggcgtgggg atggctggga gggggagcgg gggagcgcgc
      181 cggccggagc ggccgatgga cgaaaacccc cgagcggccc attgtccggg acccggtccc
      241 gggggtagaa agccccagga acggcccctc ctctcccggg cccgagtcag gagatgcgcg
      301 tggggccgtc ccacgggacc gggggggaca tccgtctcgt gccccagcgc agcggtggca
      361 ggacccgctg gctggcggtg ccggatccgg ccctcggggc cgcgctcacc gcggccgtga
      421 ccgcggtggc cggtcgggtc cagcgggtcc tgggccccga ggtgatcgcc aaccgggtga
      481 cggccgccga tccgtccgtg cccagcctgt ccctggagga ctgggtcgat gcccggaagc
      541 ggttcgcgct cgcggcccgg tccctggcgg ggcgcgggac cgggctcttg gcgctggacg
      601 tgcgcgcctg ctacgcctcg atccccccgt cgacggtcgc ccggtccctg gtccgtctgg
      661 gttgcgggcc gcgggagacc ctgagtgggt cccgagccat cggccgtgct ggccaacgcg
      721 gtgctggccc gcgccgacgg cgtgctgcgc cgagcaggcg tggcccacct gcgctgggtg
      781 gacgagttct ggatctcgat cgcgcccgga gcctcccctt cgcggctctc ggccacgctc
      841 acggcggccc tgcggccgct cggcctggcc ctggccccgg agaaggctcg attcctgccg
      901 ggctcggagg tcccgggcgt ggacggcggt tcggctcccg gctcctgcat cgggccgggg
      961 tatcatcggc gctccgatgc gcaccctgta ccgggcctcc acggtccaca cgctggccca
     1021 tcccccggtg ggggagtggg tcctggtcga cggccggcac gtggagcggg tgggctcggg
     1081 tgacccaccg agcgccgacc gggtggtgga cctgcccggc accgcggtcc tgccggggtt
     1141 cgtggatgcc cacgtgcacc tcaccgggac cggtgagcat caccgcgtcc ccgaggtgag
     1201 tcgggcgcga tcggcccagg agctgctgga gacgctgacc cggctgaccc agcaggagca
     1261 ggggccgctg ctggtccacg ggttcgacga gagcgtgtgg gagcggccgg agccgccgtc
     1321 gatcgaagcg ctggaccgga tcaccgatcg gcccctggcg gtggtgcggg tggatggcca
     1381 cctgtccctg gcgaaccggg gagccatcca ggcggccggc gctggggacg agcccggggc
     1441 cgaacgagac gccgggggag cccccacggg gaggctgacg ggggccgcca acgcgaggct
     1501 gcggcgctgg ttccagcagc acctatccga ccgcgacgtg gagcagctcc agctcgaggc
     1561 ggccagcctg gccgtgcgtc acgggatcac cgccgtccac gagatgtcga tgccggccga
     1621 gcgcgggatc cgcgacctgc agatcctgct gggccaccgg catcggctgc cggtggacgt
     1681 ggtcccctac gtggccacca cggacatcgc ccaggtcatg gatctggggc tccccggcat
     1741 cggcggcgac ctgcccgtgg acggctcgct cggcgcgcgc acggcctggc tgaacgagcc
     1801 gtaccgagcc ggggagggac gggggacccg cgccttcgat gacgaggagc tggccgggtt
     1861 cttccacgcc gggcatctgg ccggcctcca ggtgggcgtg cacgcgatcg gcgacgccgc
     1921 catcgagcag gtggtcggca cctgggagcg ggtgtaccgg accctcgact cccggcagcg
     1981 gcgccatttc cgggcccggc gacaccggat cgagcacttc gagatggccg gtcccgatct
     2041 ggtggagcgg gcggccgcct tgggcctggc catctcggtc cagccggggt tcgacgcggc
     2101 ctggggacac cccggatcgc tgtacgagca gcgcctggga ccgaaccggg cccgagacat
     2161 gaaccccttc cggtgggtcc tggaacgagg gtccgagatg ggggccgggt ccgattcccc
     2221 catcacctcg atcgatccgc tggtggggat cgccgccttc gagtcgcacc acgacccgtc
     2281 gcagcgcctg tcccgagccg aggcgatccg catctacacc agcggcagcg cccggctggc
     2341 ccacctggag gacaagaagg ggagcttggc gcccggcatg cacgccgact tctcggtctg
     2401 ggacgacgac ccggtgacgg cgccgaccct ggacgagctc ggccccatcc tgaccgtctc
     2461 gttgggccgc gaggtctacg cctcctgagg ctgcctccca ccagcgtgtt tgacagtgcc
     2521 cggaggccgg tcctacagta cgcccgcgtc accgcctcgg cggggacgca gcaggaccca
     2581 agaggctgag tagagaacgc ggcgccccgg cgctgcggcc ccaaaggtct cttgggtcct
     2641 ctgtcgttcc ccgctcccgc cgccccgccc cctctcgggc atcgcgctcc gcgggtttgc
     2701 acgccgtccc cgtgggcatc ctgtgcggga aacctctgcc tcttgaccgc gaggtcggga
     2761 acaaccgaag gcgagcgggt gtttatgccc ggaaggccct tgcggggttg cgccggggca
     2821 gtatgatgcg ccggccggaa agagtgaccg atggaagaca tcgaagagca gatcgaggac
     2881 gaacagctgg acgagccggg ggagcccggc gagatcgatg ccgacgcctc cacgccgccg
     2941 gcggcggacg atggcgatgg cgaggacgta gagtcgatcg aggacctcct ggtcaagaag
     3001 gaagcccagg aggccgagga agaagacgat tccgtcctga acatgtcccg ggaggagcgc
     3061 ctggagccgc tggcggtacg ggtggtgccg ccgcagccca ccgagttcat atgcaagagc
     3121 tgctatctgg tgaagcaccg cagccagctc aaggacaagg cgcgcatgct ctgccgggac
     3181 tgcgcctgaa ccgacccgcc ctccgcctca ccctcgccat cctgatctcg gccggatgcg
     3241 ggatactgct ctcgctggcc cttcccccgg tcgacgccgg atggatcgcc ttcctcgcgc
     3301 ccgctccctt cctgtgggtg atccgccggt cgcgtccgct ccgcggcctg ctcctggggt
     3361 tcgtgttcgg gctgagctcc ttcggagcga tcctgtggtg gatcatgcag ttcggtcagc
     3421 tggcctgggg cgccctggtg gtggtcctgg cggcctcggt gggggtcttc gggctgctgg
     3481 caccggtgct gtggcggccg agccgaccgg tggtgtcctc gctcggcctg gcctcggcct
     3541 ggacggtgac cgagtggatc cggggcatgt ggccggtcgg gggcttcacc tgggggcagc
     3601 tcggcagcac ccagacgggc aaccccttcc tgctgcccct ggcctcggtg ggcggggtgt
     3661 gggccatctc cttcgtggtg ctgctggtgg cggccctgct ggtcgaggcc ggcaccaggg
     3721 tccgctcccg cccggtcgga gcggcggggc tggtggcgat cgccgccgtc gtggtcctgg
     3781 cgccggtggc gatccagatc cctaggccga ccggtcggat cctggacgtc gcgacgatcc
     3841 aggtcgacgt gcgacgggcg gcggggctcc cgccgttgcg ggaggaccag cgtgtggccg
     3901 agctggtgat gcgcctggac cggaccctga ccgcggaccc gccggacctc gcgatctggg
     3961 gcgagagctc gctggacccg ggggccaacc tgccctcgtt ccggccgcgg gtccgctcga
     4021 cgatccggca ggtgggggtc cccacgctgg cgggcgcggt ggtggagacg ccgtcgggcc
     4081 gcttctacaa ccagagcctc ctgttcgatg gatcgggcag ggtggtgggg gagtaccgga
     4141 aggtgcacct ggtcccctac ggggaatacg tgcccttccg acgcgagctc tcctggatct
     4201 cggccctcag gcagatcccc tacgacgtga ccccggggga tcgggtgcat ccgctgcacg
     4261 tggacgggat caccttcggc aacgtgatct gcttcgagaa cagcttcccg ggcatcgatc
     4321 gtgagctggt gaaccagggg gccgggttcc tggtggtgac cacgaacaac gcgtcgtacg
     4381 gcttcagcgc ggcctcccgc cagcacgtca tcatgagcca gctccgggcc gtggagaacg
     4441 gccggtgggt cgtgcacgcg gccgtctcgg ggatcagcgc gctcatcgat ccccagggtc
     4501 gcatcgtgag cgagacgggg ctgttccagc cggccgtcct gcgcgctccg gtgcgggcct
     4561 ccacggcccg caccctgtac acgcgcctgg gggactgggt cccctggctg gccctggcgt
     4621 tcaccctggg gctcctggtg gctccccgtc gccgccggca tccggcccgg gagccgggcc
     4681 cgggaccggc ccggccgcga tcgctggtgc tgcttcccac ctacgacgag cgcgccacga
     4741 tcgcccaggt gatcgaggcc ctgctggccc tgccccaggc gctcgacgtc ctggtgatcg
     4801 acgacgcgtc gcccgacggt accgcccagg tggtgcgggc gatggcggag ggccatcccc
     4861 gggtccggct ggtggagcgc ccccgcaagg ccggcctggc ctccgcctac ctggtggggt
     4921 tccgccgcgc cctggaggag ggctacgacc tggtggtgga gatggactcg gacctgtccc
     4981 acgacccctc cgagctcccc gccctgctgg cgggggccgc ccggaaccac ctcacgatcg
     5041 gcagccgata cgtccccggc gggtcggtca ccaactggag ccggtcccga atcgcgctgt
     5101 ccagggcggg caaccggtac gcgaggctcg cgctcggctt gcccttgagc gatgccacca
     5161 gcgggtaccg cgtcttccag gcggccctgc tccgccacct ggtgcagggg ggcatccact
     5221 ccgacggcta cggcttccag atcgagctcg ccctgcgatc gtggaacgac gggttccgcg
     5281 tgggcgaggt cccgatctcg ttccgcgagc gccagcacgg cgtctccaag atctcgcggc
     5341 gcatcgtggt cgaggcgctg ctcatggtca ccctgtgggg cctccggatg cggttgggcc
     5401 gccctccggt ggtcggcccg cagaacgccc ataagggaca ttatggaacc tagagcaagg
     5461 gatggcatcc tgggctcgga tgccccctcg ggccgtccca tccccgtttc gcggacccgc
     5521 tgatctccgc aggacgtcgc tcatcggcgg cgacccgacg tagacggggt ctaggtcgaa
     5581 cggtccatgt gcggtaggta ccgttcggcg gatggtgtag gaacgcgctc cgcgtaccat
     5641 ccctcctggc gggaccgctg tcccgccaac gggctacccg gatctcgggg cttgtacgga
     5701 gggaggacga cgatgcgtgg gacgcgaagg cgcccagcgg acggccggac gcgacggaag
     5761 cggtgggagc tcatcgggct gctgctgtcg ctgaccctga tccttagtca gagcatcggg
     5821 gtggtcggcg ccgcggcgtc ggacggcccg atcagcgagc cggacgacgt ggccacggcc
     5881 accccggctg tagacgcacc ccaggcggcg ccctcgccga gcgaagcccc accggcggcc
     5941 gaggatccgg ccccggggct cggcgccgag ccgggggcca cgacctccga gcagaccccc
     6001 tcctccgggg ggccgcccgc cgggggcgac ggagctggga ccgatcagca gagcccggag
     6061 ccgagcgcgg acgccacgaa gaccgcctcg cggaccggcg gcggcggcgg ttcggttgcg
     6121 ccgacgctca tctcctcccc ctcctcggga tcgctgaacg tggacctgga tcagttcgcg
     6181 aaccagcccg gcaagggctg gcagaacggc gacctgaacg ggaacaactc ggcgtaccac
     6241 gagggggacg tggtcccctt ccgcctggcg atcgagggcc tgagcgccgg gtcccacacc
     6301 atccacctca gctacgactt cacggccggc gggcacgagg cctacgactt cctggccacc
     6361 tgggacgcct cggagagccc gagcctctgc gcctcgggcg gcggggcggt ctcctcgatg
     6421 tgtccgggcc tgccctcggc ccacacgcac gtcttcccga gcgatccctt cgcgcccggc
     6481 tcccccacca aggccggact gacggtcgcc ggggcggagt ccttctccgg cctgtcccgg
     6541 aacctgacga tgtacggggg gacgatcgac tcgatcggcg gccctgcgca ctcgggaccg
     6601 gtgtcgggca acagcaccgg tgacatcacc gtgcacttca cctcctccgg cagcgcggtc
     6661 ctgttcgcgt ggggcgggca cctggccgag tccgcctact gggtgcggac caacggggac
     6721 ccgaacgggg cctcgcaggt gtccggggca ccctggcaca tgcggaccca gtccctggac
     6781 gggtccggga acaagaacca ggaccggagc atccagccca gcgcgatcgt ggccctgccg
     6841 gcgctcgcca tccagaagag cgccgacgcg ggcaccgtgc agatcggcga ccagatcggc
     6901 tactccatca cggtgacgaa caccgggaac gtgggcgtga gcaacgtctc ggtgcaggac
     6961 gcgctgccgg gcggctccgg gatcgactgg agcgtggccg gcaccaccgg cgacaccaac
     7021 gggctggact gctcgatcgg cgggtcgccg cccagccagg cgctctcctg caccaaggac
     7081 agcctggcca agggcggcag cttcacggtc cacgtgacca gcccgaccag ggggtcggcc
     7141 tgcggcacct acgacaacac ggccagcgcc tccgcggacg gggtcgaggt cgtgcagtcg
     7201 cagacggcga ccatcgtggt cgagggctgc ggtaccgacc tcaagatcac caagaccgcg
     7261 tcgagcgacg cggtccccta cggatcgtcg ttttcgtaca cgctcaccgt ccgcaaccag
     7321 ggcagcgggc aggccaccgg ggtcgtggtg atcgacgatc tggccgacag cctcaccggg
     7381 gtctccgcga gctacgacgt gaaccccggg agccccgggg acacgggtgc ctgctcggtc
     7441 gacaccggca acctggtgac ctgccaggtc gccacgctcg cccccgacga cggggcggcg
     7501 aacgggcccg acacgatccg gatccagatc caggccacgg cccccgacac ctgcggcgcc
     7561 atcacgaaca cggcccacgt cagctcggac caggacgcca cgggctcgga cagcaacgag
     7621 gttcagatcg acgtgaccgg ctgcgtcacg cctccggccc cgatcggcat ccggatcgtg
     7681 aagggaggac cggccctggc gcacgtgggc gacacgatca cctacacgtt cgacgtcagc
     7741 ctgacccagt cgacgccgct ctcgaacatc gcggtcagcg acccgatgtg cagcaccgca
     7801 ccggggctgg actccaaggc cggcgggaac caggacgacg tgctggagcc gggtgagacc
     7861 tggcgttact cctgcacgca cgtggtcacc tccagcgatc cagacccgct gccgaacacc
     7921 gccacggtca cggggacctc ggaggacgag cggaccgcga ccgatcagga cagccacctg
     7981 gtggacctga tccacccggc gatccggatc gtgaagaccg cgagcgccag gtccggtgag
     8041 ccggggggat cggtgaccta cacctacgtc gtcacgaaca cgggcgacgc gaccctgttc
     8101 gcgatcagcg tggacgacga cgtgatcggg cacatctgcg atatggcgtc gctcgatccg
     8161 ggtgcaagct ccacctgcac ggccggctac gtgctgccgg atcggagcgg tccgatcgac
     8221 aacgtcggca ccgcggtcgg atccgaccag accggggcca aggtgagcga ccacgatagg
     8281 gcctccgtgg ccgtggtcct cggcaccacc gtcacgcctc cgcccaccac caccccgccc
     8341 aagggcacgg ccttcaccgg agcggccgac gccgtgccgc tggcggccct ggcgttggcg
     8401 ctcctcctgg ccgggtcggg gatcctcttc gccagccggc ggaggaggca gggctctgcg
     8461 cccgattgac ccccgagggg atcgggggga acggcccggg catctcgccc gggccgttcc
     8521 ttgtcccggg gccgtcccgt gccccgctcc cccactcggt cagccgaccg ggacggggcg
     8581 ttctccgagg ccgaggaggc accgtcgccc cccgctcccc gtgcatccct cgggccgagg
     8641 ggccgtgtcc tctcccccgg ggacagggga gcgggacgct accagcggag cttcagatgg
     8701 cgggccgcgc gggcctcgtc gagcctgcgc accggcgtgc tcaccggggc ctcgtgaagg
     8761 agcgcgggat ccgtggcagc ctcctcggcc gcctgcacca tggccgcggc gaaggcgtcg
     8821 agcgtctccg tgctctcggt ctcggtgggc tcgaccatca tcgcctcctc caccaccaac
     8881 gggaagtaca cggtggacgg atggaagccc agatcgatga cccgcttggc gacgtccacg
     8941 gcgcggatgc ccgtccgctc cttcagcggc ctgcctgtgg ccacgaactc gtgcatcgga
     9001 cggcccgggt acgcggtggg gaacgccggt cccagcagct ccgccaggta gttcgcgttc
     9061 aggacggcca ggtccgccac ctccttcagc ccatctcgcc cgtgcaggaa cacgtacgcg
     9121 tacgcgcgga ccaggacccc gaagttcccg tggaagccgt gcatccgccc gatggagcga
     9181 gccccgcctt cctgcaggtg gaaacgcccg tccgcgtccc gctccacgag cgggccgggg
     9241 aggtagggaa ccagccggtc caccaccccc acgggaccgg ccccgggacc cccgccaccg
     9301 tgcggggtgg cgaaggtctt gtgggtgttg atgtggacga tgtcgaagcc catgtcggcc
     9361 ggccgggccc ggcccaggat cgcgttcagg ttggcgccgt cgtagtagac gagccccccg
     9421 gccgcgtgga ccacttcggt gatccgctcg atctcctcct cgaacaggcc gagggtgttg
     9481 gggttggtca acatcagccc ggccacgtcc tcgtccacga gccgctcgag cgcgccgacg
     9541 tcgacgaggc ctcg
//
LOCUS       DASQQQ010000203         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_229470, whole genome shotgun sequence.
ACCESSION   DASQQQ010000203 DASQQQ010000000
VERSION     DASQQQ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229470"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VE646_07760"
     CDS             complement(<1..391)
                     /locus_tag="VE646_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79920.1"
                     /translation="MDDFLARLRRGSIGRVLVLYVGAAWLILQVVGTVNDLVHLPAWI
                     GPTAIALLVIGLPITVATAWVQGDPATSELEQAGEVPNDWELGLRELTRSVRLGRLPY
                     LNWARVSVGGLVVFSLLFGVGGLYAVFR"
     gene            complement(563..1174)
                     /locus_tag="VE646_07765"
     CDS             complement(563..1174)
                     /locus_tag="VE646_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79921.1"
                     /translation="MLKRLSRPTVLAAVVLLGACGDQDPLSPDGEPGPAVAAQVARPA
                     LVNGEAMVPITWTYRVSPIYDGSMVACPGGFVFPVHWSFDARLTHLGRLDRKGSTATF
                     TACAAGEDPQLGPYFAGDAGVHLTGPQGDAVDLEGTLTLLLAAGYATGDWDIVGGAGR
                     FAGARGHIDTVEYPEEDGSGSAGSGTGMITRPRAVTGGRPGSS"
     gene            complement(1299..>2132)
                     /locus_tag="VE646_07770"
     CDS             complement(1299..>2132)
                     /locus_tag="VE646_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79922.1"
                     /translation="YISAGIALGAGRPREALELYSTLDPHCPWAAEWVGPWNDWTAAF
                     HLLDEHRRELKEARRARQWHPDRLEALFIELRALAALGRLDAVREDLARTAEMASERG
                     WTPGTVMRRTAAELRVHGHAAAADQALEAALAWYEARPAEDRSRPAYRMAYYDALFMS
                     GRLRQAEQLAAGFHPDRPGSPDLLGREGVLAARMGASGRAREIAALLEAVRGPYQRGS
                     VDYWLAAIAAWSGEPEHALTLLRRALDQGRPRGIALHADPFVEPLWPVPAFQVAVAED
                     R"
BASE COUNT          329 a          778 c          751 g          274 t
ORIGIN      
        1 cgcggaacac cgcgtacagt ccccccacgc cgaacagcag cgagaagacc accagacctc
       61 cgacggagac ccgcgcccag ttcaggtacg ggaggcggcc cagcctcacg gaccgcgtca
      121 gctcgcgaag cccgagctcc cagtcgttcg gcacttcgcc ggcctgctcc agctcggatg
      181 tcgccggatc cccctgcacc caggcggtcg ccaccgtgat cggcagaccg atgaccagga
      241 gcgcgatggc cgtcggcccg atccacgcgg gcagatgcac caggtcgttc accgtgccga
      301 cgacctgcag gatgagccac gcggcgccca catacaggac gaggacgcga ccgatgctgc
      361 cgcgcctcag ccgtgcaagg aaatcgtcca ctgcggcccc tcgaatggag gtggagcgag
      421 gccagggtgg cgccggagga acaagaataa tgccgcctgc cggggccgaa agcaacggac
      481 cgttgatcgc ccccggagcg aagacgtccg ggggcgttct tcatggccgg gatccggtcg
      541 ccgtcgggcg gaccctctcg ccttacgagc tgcccggacg gcctcccgtc acggccctgg
      601 ggcgagtgat catcccggtg ccgctccccg cggagccact cccgtcctcc tcggggtact
      661 cgacggtgtc gatgtgcccg cgggcgcccg cgaagcgacc ggcgccgccg acgatgtccc
      721 agtctccggt ggcgtatccc gccgccagca gaagcgtgag cgtgccttcg aggtccaccg
      781 catcaccctg tgggccggtc aggtgcacgc cggcgtcacc ggcgaaatac ggcccgagct
      841 gggggtcctc gccggccgcg caggcggtga acgtagccgt gctccccttc cggtccaggc
      901 ggcccaggtg cgtgagccgg gcatcgaagc tccagtgcac cgggaagacg aacccgcccg
      961 ggcacgcgac catgctcccg tcgtagatcg gcgagacccg gtaggtccag gtgatcggca
     1021 ccattgcttc cccattcacc agcgccggcc gcgctacctg cgctgcgact gccgggccgg
     1081 gctccccgtc gggcgacagg ggatcctgat cgccgcaggc accgagcagg actacggcgg
     1141 cgagaacggt cgggcgcgag agtcgcttca gcatggcttc ctcctcggaa tgcggtctca
     1201 tgcccccggc tctgtgtcgg gtggcgtgat caggatgggg cgggggtcgt catagcgacg
     1261 tgcgtcgccc cgtcacaagg ggaggaatgg cgggacggtc agcgatcctc cgccacggcc
     1321 acctggaacg cgggaacggg ccagaggggc tcgacgaagg gatcggcgtg cagggcgatc
     1381 ccccggggcc tgccctgatc cagggcccgc cgaagaagtg tcaaggcgtg ctccggctcg
     1441 cccgaccagg cggcgatggc ggcaagccag tagtccacgc tgccgcgctg gtacggcccc
     1501 cggacagcct ccaggagagc cgcaatctcg cgggcccggc ccgaggcacc catgcgggcc
     1561 gccaggacac cttcccggcc cagcaggtcg ggcgacccgg gtcggtcggg gtggaacccc
     1621 gccgccagtt gctcggcctg acgcagccga ccgctcatga acagggcatc gtagtaggcc
     1681 atccggtacg cagggcgcga gcggtcctcc gccggtcgag cttcgtacca tgcgagggcc
     1741 gcctcgaggg cctggtcggc ggccgcggcg tggccgtgga cgcgtagctc ggccgccgtc
     1801 cggcgcatca cggttccggg cgtccagccc cgttccgaag ccatctccgc agtccgggcc
     1861 aggtcttccc ggaccgcgtc gaggcgcccg agcgccgcca gggccctgag ctcgatgaac
     1921 agcgcttcaa gccggtccgg gtgccactgc cgagcgcgac gagcctcctt cagctcccgc
     1981 cggtgctcgt ccagcaggtg gaacgccgcg gtccagtcat tccacgggcc aacccactcg
     2041 gcggcccacg gacagtgcgg gtcgagagtg gagtagagct cgagggcctc gcgcggccgg
     2101 ccggcgccga gggcgatccc ggccgagatg ta
//
LOCUS       DASQQQ010000204         6078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_154130, whole genome shotgun sequence.
ACCESSION   DASQQQ010000204 DASQQQ010000000
VERSION     DASQQQ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154130"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1681)
                     /locus_tag="VE646_07775"
     CDS             complement(<1..1681)
                     /locus_tag="VE646_07775"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HZD79923.1"
                     /translation="MTPARPNDSLPKSTGAAGARSALRLLSFPLLLAASAFSVVPWAA
                     AQTIAADAAAQGGAPARPGLRAGRIEGPIHLDGRLDEPAWTEADSIPHLTEIEPTEGG
                     RPSARTVVRVLADEHQLIFGIVAYDPDPSGIVAHSVRRDASLRGEDNMTVVLDPFLDG
                     RSGVVFRLNPNGARYDALVSRRGEGENADWDAVWDARAVRTASGWSAEIWIPVRSLTF
                     DSGLRRWGLNIQRRIERLQETDRWASPVLDYSVTQTSRAGLLTGLPEFSVGLGLSVRP
                     YVTAGGGIPAPDSDLETQEDQGIDIGQRIGTNLEATITVNTDFAETEVDARQTNLTRF
                     PLFFPEKRAFFLQGSDIFDFSLGLGDNLIPFFSRRIGLVEGRTVPIDIGAKLAGRAGD
                     TNLGALAVRMGDVRGLAPANTMGVVRVEQNVLSESSAGFLATFGDPLGRGGSWLAGPD
                     VTYQTSHFRGDKNFLVGVWALAMDREGLTGVRSAWGLKVDYPNELWDVSLTHRHIGTD
                     FDPSLGFVPRPGTNQVNLGVDYRPRPAWSLVRQMFFQLEGSLFTRLDGSLQS"
     gene            complement(1952..4432)
                     /locus_tag="VE646_07780"
     CDS             complement(1952..4432)
                     /locus_tag="VE646_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HZD79924.1"
                     /translation="MTSGAAAPSAATGTTGWRPWLEEPASRPGRRRIRAHIGGLHCSL
                     CTGTIEKALVRRPGVDRVAVSLTHEQALVEYDPEVADPDALMQTLLDIGYDLQDPRKV
                     EPFEEQERKLVREGSRFLTALAASFGALALIAPAGGWSLALPAVVYATLLAFAWALLQ
                     PRGRAVQAAGVAGLAALGVALYALKLAGPLASWAAPLTGALALGVFLGPGRSFLAAAA
                     QSLRRGILNQHVLVEIGALAGLAGGTLGLALRPAGYPTASFFAVAVMVLTYHRFSEWL
                     ALIVKTRSSQAVRKLLDLTPRTARRLRNGAEETVPVEALAVGDRVRIRPGERVPADGL
                     VLDGHSAVDESVLTGEPVPVEKAAGEAVVGGAMNGAGTLLVEVTAVGEESFLARVVRS
                     VEEARALKPGLLHLVDRVLQIYTPAVLTLSLLALAGWLAVPWLVTGAPDVRRAVFAAL
                     SVLVMGYPCAIGISAPLSIVRGAGDAANAGILMRTGEAFQALRLATHVVFDKTGTLTV
                     GEPRVMELDPAPGVEERRLLAVAAAAEAGSEHPLGEAVVRAAVGAGLVLPDAADFAAL
                     TGRGVVARLEAGVVRVGSPEWLAEEGTGSGDVDELRRLQERGWTVIGAVLDERFLGWI
                     AVGDAVREDAAGAVADLQRDGLKTVLLTGDHARTAERVARETGIETVWARVRPEEKAE
                     HVRGLQAGGASVAMVGDGINDAPALMQADTGIAMGSGTDIAIDSADIIVLNDRLASVP
                     KAWRISRRGYTRMVRNVVLAFSFNGVGVPMAATGLIHPVWAMIAMAASVTAIFVHSLW
                     GRPELFFGAVQGVGRGERGDHPAVEGAG"
     gene            complement(4449..5114)
                     /locus_tag="VE646_07785"
     CDS             complement(4449..5114)
                     /locus_tag="VE646_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008892247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HZD79925.1"
                     /translation="MWLVNPGPEPWNLAALALGLGLLGFIEPCTVGSSLLFVKYLEDR
                     DRAAKLRTTLAFAATRSILLGVLGAGAAVLGSAFLGLQHGFWILLGTLYVAVGIAYLA
                     GGQRLLVRTLGLPLSRIDGTKGGAALGVLFGLNVPACAAPLLGAVLTATLGTRAAMQG
                     FASLAVFGLALSLPLAAAVLSASGSRWLDRIAGLSRRAPLWTGLAFVALGIWSVYFGL
                     AVR"
     gene            complement(5102..5542)
                     /locus_tag="VE646_07790"
     CDS             complement(5102..5542)
                     /locus_tag="VE646_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HZD79926.1"
                     /translation="MFFIGKLAERTGISRDAIRYYESLGVLPQPERTGAGYRVYGPAD
                     VERLEFIGQAQVLGLTLDEIADVLEIVGEGRAPCDHVRERLAGHLERTRERIRELRFL
                     ERRLASALAGADATDVPDGAVCRCRIIEAAVADHAVDPVERCGS"
     gene            5714..>6078
                     /locus_tag="VE646_07795"
     CDS             5714..>6078
                     /locus_tag="VE646_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79927.1"
                     /translation="MFVHALVAAALAGPVVPPEPACALAGPPVRAGWSGAVPDATEAA
                     APLRVVEDVPLPGGPSRFDYQSLEPASGRLFIAHMGAGRLVVFDVRARRVLADLDGFP
                     TVTGVLAVPAQRRVYASAAG"
BASE COUNT          893 a         2308 c         2102 g          775 t
ORIGIN      
        1 agctctggag gctaccgtcc agccgcgtga acagcgatcc ctccagctgg aagaacatct
       61 gccgcacgag gctccaggcc gggcgggggc ggtagtccac gcccaggttg acctggttcg
      121 tgcccggccg ggggacgaag cccagggagg ggtcgaagtc ggtcccgatg tgccgatggg
      181 tgagggagac gtcccacagc tcgttcggat agtccacctt gagcccccag gcggacctga
      241 ccccggtgag cccctcccgg tccatcgcca gcgcccagac gccgaccagg aagttcttgt
      301 cgcctcggaa gtgggaggtc tgataggtga catcgggacc ggccagccag ctcccacccc
      361 gtccgagagg atcgccgaag gtggcgagga agccggccga ggactccgag agcacgttct
      421 gctcgacccg gacgaccccc atcgtgttcg ccggggccag cccccgcacg tcccccatcc
      481 gcaccgcgag cgccccgagg ttggtgtcgc cggcgcgtcc ggccagcttg gccccgatgt
      541 cgatggggac cgtgcgcccc tcgaccaggc cgatccggcg gctgaagaag gggatgaggt
      601 tgtcgccgag ccccaggctg aagtcgaaga tgtcggagcc ctgcaggaag aacgcccgtt
      661 tctcggggaa gaagagcggg aagcgggtaa ggttcgtctg tcgggcgtcc acctcggtct
      721 cggcgaagtc ggtgttgacg gtgatggtgg cctccaggtt ggtcccgatc cgctgcccga
      781 tgtcgatgcc ctggtcctcc tgcgtctcca ggtccgaatc gggagccggg atcccgccgc
      841 ccgccgtgac gtagggccgc acgctgagcc ccagtccgac gctgaactcc ggaagccccg
      901 tgagcagccc cgctcgactg gtctgcgtga cgctgtagtc caggactggg ctcgcccagc
      961 ggtccgtctc ctgcagccgc tcgatgcgcc gctggatgtt gagcccccag cgccgtagcc
     1021 ccgaatcgaa cgtgaggctc cggaccggga tccagatctc cgccgaccag ccactcgcgg
     1081 ttcgcaccgc tctggcgtcc cagaccgcgt cccagtccgc gttctccccc tcgccccggc
     1141 ggctgaccag cgcgtcgtag cgggccccgt tcgggttcag tctgaacacg acgccagagc
     1201 ggccgtccag aaagggatcc aggacgaccg tcatgttgtc ctcacccctc agcgaggcat
     1261 cgcgccggac gctgtgggcc acgattcccg acgggtccgg gtcgtaggcc acgatcccga
     1321 agatgagctg atgctcgtcg gcgaggaccc ggaccacggt ccgtgccgag ggccgaccgc
     1381 cctcggtcgg ctcgatctcg gtcaggtgcg ggatggagtc ggcctccgtc cacgccggct
     1441 cgtccagccg tccgtccagg tggatcggac cttcgatccg gccggcccgg agccccggac
     1501 gggccggcgc accgccctga gcggccgcgt cggcggcgat cgtctgggcc gccgcccacg
     1561 ggacgacgga gaacgcggag gccgcgagca ggagcggaaa cgagagcagc cggagcgccg
     1621 agcgggcgcc cgccgctccc gtgctcttcg gcaaggagtc gttcgggcgt gcgggagtca
     1681 cgaccatcgt cgccgccggc ggttgggccc gcgacgggag aatcgacaga ggctcgttca
     1741 cgccgcgcgg ggcacaggtc agcgggcgcc tgcggaccgg gcctgctcga atgtgaaggc
     1801 gccggtggac gacgacttgc gcatgcctgg cctcgtctat ccaagagtgg cggatgcgga
     1861 gaatcctaca ggttacgctg catgaaaggc tccacgaagc aagggctccc gggccgctcc
     1921 gtcgaggcac cgcgccccga cggcgttgcc ctcacccggc tccctccacg gccgggtgat
     1981 cgccgcgttc cccgcgtccc acaccctgca cggcgccgaa gaagagctcc ggccgccccc
     2041 acagggagtg gacgaagatc gcggtgacgc tggcggccat cgcgatcatc gcccacaccg
     2101 ggtggatcag gccggtggct gccatgggca cgcccactcc gttgaatgag aaggcgagaa
     2161 ccacgttccg gaccatgcgg gtataaccgc gccggctgat ccgccaggct ttcggcacag
     2221 acgccagccg gtcgttcagc acgatgatgt cggcggagtc gatggcgatg tcggtcccgg
     2281 atcccatcgc gattccggtg tccgcctgca tgagggccgg cgcgtcgttg atcccgtcgc
     2341 cgaccatcgc cactgaggcg cccccggcct ggagcccccg cacatgctcg gccttctcct
     2401 cgggacgcac ccgcgcccat accgtctcga tcccggtctc ccgggccacc ctctcggccg
     2461 tacgggcgtg atcccccgtg aggagcacgg tcttcagacc gtccctctgc aggtccgcca
     2521 ccgccccggc cgcgtcctcg cgcaccgcat cccctaccgc gatccagccc aggaaccgtt
     2581 cgtccaggac ggccccgatc accgtccacc cccgctcctg caggcggcgg agctcatcga
     2641 cgtccccgga cccggtgccc tcctccgcca gccactccgg actgccgacc ctcacgacgc
     2701 cggcctcgag gcgcgccacc accccacgac ccgtcagggc cgcgaagtcg gccgcatcgg
     2761 gcaggaccag gccggccccg acggccgccc ggaccaccgc ctcgcccagg gggtgctccg
     2821 agccggcctc cgccgccgcg gccacggcca gcagccgcct ctcctccacg ccgggcgccg
     2881 ggtcgagctc catcacccgg ggttcgccca cggtgagcgt gccggtcttg tcaaagacca
     2941 catgcgtcgc cagccggagc gcctggaacg cctcgccggt gcgcatcagg atgccggcgt
     3001 tggccgcatc acccgcgccc cgcacgatgg agaggggcgc cgagatcccg atcgcgcacg
     3061 ggtagcccat caccagcacg ctgagcgcgg cgaagacggc gcgtcgcacg tcgggcgcac
     3121 cggtcacgag ccagggcacc gccagccagc cggcgagggc gagcagggag agggtgagca
     3181 cggccggcgt gtagatctgg aggacgcgat cgaccagatg gaggagcccg ggcttgagcg
     3241 cccgcgcctc ctccacgctc cggaccaccc gggccaggaa gctctcctcc cccaccgcag
     3301 tcacctccac gagcagcgtg cccgcgccgt tcatcgcgcc gcccacgacc gcctcgcccg
     3361 ccgccttctc cacgggcacc ggctcgccgg tgagcaccga ctcgtccacc gccgaatggc
     3421 cgtccagcac gagcccgtcg gcggggaccc gctcgcccgg ccggatgcgg acgcggtcgc
     3481 cgaccgccag cgcctcgacc ggtaccgtct cctccgcccc gttccgcagg cgccgggcgg
     3541 tccgcggggt caggtcgagc agcttgcgca ccgcctggga gctccgcgtc ttgacgatga
     3601 gcgcgagcca ttccgagaag cgatgatacg tgagcaccat gaccgccacg gcgaagaagg
     3661 aggccgtcgg gtagcccgcc gggcgaagcg ccaggcccaa cgttccaccg gccagcccgg
     3721 ccagcgcgcc gatctccacc agcacgtgct ggttcaggat cccgcggcgg agcgactggg
     3781 cggcggcggc gaggaagctc cgacccgggc ccaggaagac gcccagcgcc agggcgcccg
     3841 tgagcggggc cgcccacgac gcgagcgggc cggccagctt cagcgcgtag agggcgacgc
     3901 cgagcgcggc gaggccggcg acgccggccg cctgaacggc gcgaccgcgg ggctggagga
     3961 gggcccaggc gaaggcgagg agcgtggcgt acacgaccgc cggcaaggcc aggctccagc
     4021 cgccggcggg agcgatgagc gcgagggcgc cgaagctcgc ggccagcgcg gtcaggaacc
     4081 gcgagccctc ccggacgagc ttccgctcct gctcctcgaa cggctcgacc ttccgggggt
     4141 cctggaggtc gtagccgatg tcgagcaggg tctgcatcag cgcgtcggga tccgcgacct
     4201 ccggatcgta ctccaccagc gcctgctcgt gggtgaggct caccgccaca cggtccactc
     4261 ccggccggcg aaccagcgcc ttctctatgg tgcccgtgca caaagagcag tgcaggccgc
     4321 cgatgtgggc ccggatgcgc cgacgcccgg gacgtgacgc gggctcctcg agccagggcc
     4381 gccagccggt cgtcccggtc gccgccgagg gtgcggcggc tccgctcgtc atgtcgctcc
     4441 ctccgggctc accggacggc cagtccgaag tagaccgacc agatccccag cgccacgaag
     4501 gcgaggccgg tccagagcgg cgcgcgccga ctgagccccg cgatccggtc cagccaacgg
     4561 gacccgctgg ccgagagcac ggccgcggcc agcggcaggg acagggcgag cccgaagacg
     4621 gccagcgacg cgaagccctg catggcggcc cgggtcccca gcgtcgcggt gagaacggcg
     4681 cccaggagcg gcgccgcgca ggccggcacg ttcagcccga agaggacgcc gagcgcggcg
     4741 ccgcccttcg ttccatcgat ccgggagagc ggcaggccca gcgtccggac cagcagccgt
     4801 tgccccccgg ccaggtaggc gattcccacc gcgacgtaga gcgttcccag caggatccag
     4861 aagccgtgtt gaaggcccag gaaggccgag cccaggaccg cggcgcccgc gcccaggacc
     4921 ccgaggagga tgctcctcgt cgccgcgaag gccagcgtcg ttcggagctt ggccgcccgg
     4981 tcgcggtcct cgaggtactt cacgaacagc aggctggagc ctacggtgca gggctcgatg
     5041 aacccgagca ggccgagccc gagcgcgaga gccgccaggt tccacggctc ggggcccggg
     5101 ttcacgagcc acaccgctcg accgggtcga cggcatggtc cgcgaccgcc gcctcgatga
     5161 tccggcagcg gcagacggcg ccgtccggca cgtccgtcgc atcggcgccc gccagtgcgc
     5221 tcgccaggcg acgctccagg aaccgtagct cccggatccg ctcgcgggtt cgctccaggt
     5281 gcccggccag ccgttcccgc acatggtcgc aaggcgcgcg cccttcgccc acgatctcca
     5341 ggacgtccgc gatctcgtcg agcgtcagcc ccagaacctg cgcttggccg atgaactcca
     5401 gccgctccac gtccgccggg ccgtagaccc gatagcccgc gcccgtgcgc tccggctgcg
     5461 gcagcacgcc gagactctcg taatagcgga tcgcgtcccg gctgataccg gtccgctccg
     5521 ccagcttgcc gatgaagaac accttctctc cgatcatgtc cgctctcgct ctccctcgat
     5581 cgtccctcac aaacagaagg taaaccctgg ggcccgctcc agggtcaagg gtccgctcga
     5641 acggcgcccg cgctctgcct ggatccgcaa cggccgtcgt gcgggccgga cgcggtgagc
     5701 gtaggttcga gccatgttcg ttcacgcgct ggtcgcggcc gccctcgccg gacccgtcgt
     5761 ccctccggag ccggcctgcg cgctggccgg gccgccggtc cgcgccggct ggtccggcgc
     5821 cgtccccgac gcgacggagg cggcggcgcc gctccgggtc gtggaagatg tgccgctgcc
     5881 gggcggcccg tcccggttcg actaccagag cctggagccg gcgtccggaa ggctcttcat
     5941 cgcccacatg ggcgccggcc ggctggtcgt cttcgacgtg cgggcccgca gggtcctcgc
     6001 cgacctggac gggttcccga cggtgaccgg cgtgctggcc gtgcctgccc agcgccgcgt
     6061 ctacgcgtcg gcggcggg
//
LOCUS       DASQQQ010000205         2901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_181863, whole genome shotgun sequence.
ACCESSION   DASQQQ010000205 DASQQQ010000000
VERSION     DASQQQ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181863"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..902)
                     /locus_tag="VE646_07800"
     CDS             complement(3..902)
                     /locus_tag="VE646_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZD79928.1"
                     /translation="MAYWVPMYPETALSHLQALERPRAIVEPVLEGFLVARMEEMAAI
                     DRRAAALVGEILRLLAAGGKRIRPAFCYWGYRAGGGTEDATIAKAAAALELFHTFALI
                     HDDLMDESDRRRGVPSVHVRLAEDHPGPADRAERYGRSGAILIGDLAAVLADELLLAS
                     EVPADRLLAALRRYDQMRIEVAAGQFLDVAGVARDEPSALRVAALKTGAYTVEGPLHI
                     GAILAGASLEAMAGLSAYARPLGEAFQLRDDVLDHGEGSAPGATAATVDELLDRADAA
                     LDGPHLVPEAVAALRSLGGALRL"
     gene            1028..2836
                     /locus_tag="VE646_07805"
     CDS             1028..2836
                     /locus_tag="VE646_07805"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HZD79929.1"
                     /translation="MGRSDRFRTLALSSAAATAVLIGWGGLVRATNSGDGCPDWPKCF
                     GHWIPPFEYHALIEYTHRSLAVVATVLIGLQAIAAFRRHRHQPIVFWASVLVVPLFLV
                     QAALGGVVVKTGLAPGWVTVHFAVAMLVLADLVVALVASLGAERRARTGSFARLALWT
                     AAATYALLLAGTYVRARDAGVVFQDWPLMDGKLVPALGGAATVMFVHRVLAAAVTLLV
                     LWVAIRARTLPGRPRSLVILSTALIAAFAAQVMVGAANVWTGLEAWARAAHVMLAAAI
                     WAIAVGLAVWARYVPSPSEGEGARATPDEGVMARPSVRERTTAYVRLTKPRIIELLLV
                     TTVPAMILAERGIPSLGLVLATLGGGTLAAGSANVINCYLDRDIDEVMRRTRRRPLPA
                     NEVSPEQALRFGYALGAVSFLFLSIVVNVLAATLALSAIAFYVFVYTMWLKRTSVQNI
                     VIGGAAGAVPALVGWAAVTGSVGLPAWVLFAIVFVWTPPHFWALSMQYRTDYAAAGIP
                     MMPVVRGEEETRRQILLYTLVLVGTSLLLYPLGHMGPVYLAASVILGGMFLHRALRLW
                     RERSRGLAIGLYRFSILYLGLLFAAVAVDAVLPLAR"
BASE COUNT          330 a         1069 c         1054 g          448 t
ORIGIN      
        1 cgtcatagcc gcagcgcccc gcccagggag cggagcgccg ccaccgcctc ggggaccagg
       61 tgcgggccgt ccagggccgc gtcggcccgg tccaggagct cgtccaccgt ggcggcggtg
      121 gctcccggcg cagagccctc accgtgatcc agtacatcat cacggagctg gaacgcctcg
      181 cccagggggc gcgcgtaggc cgagagcccc gccatcgcct ccagcgaggc ccccgcgagg
      241 atggccccga tgtgcagcgg tccctccacc gtgtacgcgc cggtcttcag cgccgccacg
      301 cggagggcgg agggctcgtc ccgggccacc cccgcgacgt cgaggaactg acccgccgcc
      361 acctcgatcc gcatctggtc gtagcggcga agcgccgcca gcaggcggtc ggctggcacc
      421 tcgctcgcca gcaggagctc atcggccagc accgccgcca ggtccccgat caggatggcg
      481 cccgagcgcc cgtaccgctc ggcccggtcc gccggcccgg ggtggtcctc ggccaggcga
      541 acgtgcacag aaggtacgcc ccgacgccgg tcgctctcgt ccatcaggtc gtcgtggatc
      601 aacgcgaagg tgtggaacag ctcgagcgcg gccgcggcct tcgcgatcgt cgcgtcctcg
      661 gtccccccac cggcccggta cccccagtag cagaaggccg gacggatgcg cttgccgccg
      721 gccgcgagga gccgcaggat ctcccccacc agcgcggcgg ccctgcgatc gatcgccgcc
      781 atctcctcca tccgtgccac caggaagccc tccagcaccg gctccacgat ggcgcgggga
      841 cgttccagcg cctggaggtg ggaaagggcc gtttccgggt acatcgggac ccagtatgcc
      901 aagccggctc cgccacgggc ggcccccatc ggatcccacg ggctcgccgt gccgccctcc
      961 ggggcagacg gaaggagggg cctcgggcta tagtggtcgc cggtccgccc gggcgggccg
     1021 ccgctccatg ggtcgatccg atcgcttccg cacgctcgcg ctctccagtg ccgccgccac
     1081 ggcggtgctc atcggatggg gtgggctggt gcgggccacg aactcggggg acggatgccc
     1141 ggactggccc aagtgcttcg ggcactggat cccgcccttc gagtaccacg ccctgatcga
     1201 gtacacgcac cggtcgctgg cggtggtggc caccgtcctg atcggcctgc aggccatcgc
     1261 ggccttccgt cggcaccgcc atcagccgat cgtgttctgg gcgtcggtgc tcgtggtgcc
     1321 gctcttcctg gtgcaggcgg ccctgggcgg cgtggtggtg aagacgggcc tggcgcccgg
     1381 atgggtgacc gtgcacttcg cggtggccat gttggtgctg gccgatctgg tggtggcgct
     1441 ggtggcctcg ctgggcgccg agcggcgtgc gcgcaccggt tcgttcgcgc ggctcgcctt
     1501 gtggacggcg gcggccacgt acgccctgct gctcgcgggc acctacgtcc gggctcggga
     1561 cgccggggtg gtcttccagg actggccgct gatggacggg aagctggtgc cggccctggg
     1621 cggggcggcc accgtgatgt tcgtccaccg ggtcctcgcc gcggcggtga cgttgctggt
     1681 cctgtgggtg gccatccgag cccggaccct ccccggccgg ccccggtcgc tggtgatcct
     1741 gtccaccgcg ctgatcgcgg ccttcgcggc gcaggtgatg gtcggagcgg cgaacgtgtg
     1801 gaccggactg gaggcatggg cgcgggccgc ccacgtcatg ctcgccgcgg ccatctgggc
     1861 catcgcggtg ggcctggccg tgtgggcccg gtacgtcccc agcccctccg agggggaggg
     1921 cgcgcgggcc accccggacg agggcgtgat ggcccggccc tcggtccgcg agcgcaccac
     1981 cgcctacgtg cggctgacca agccccggat catcgagctc ctgctggtca ccaccgtgcc
     2041 ggcgatgatc ctggccgagc gggggatccc gtcgctcggc ctcgtcctgg ccaccctggg
     2101 cggggggacc ctggccgccg ggtcggccaa cgtcatcaac tgctacctgg accgggacat
     2161 cgacgaggtc atgcgcagga cccgccgtcg gccgctgccg gccaacgagg tctcgcccga
     2221 gcaggcgctc cggttcgggt acgccctggg ggcggtgtcc ttcctgttcc tgtcgatcgt
     2281 ggtgaacgtg ctggccgcca ccctggccct gtcggcgatc gccttctacg tcttcgtcta
     2341 caccatgtgg ctcaagcgga ccagtgtgca gaacatcgtg atcggcgggg cggccggcgc
     2401 ggtgcccgcc ctggtgggct gggccgccgt caccggctcg gtggggctgc cggcctgggt
     2461 cctgttcgcg atcgtgttcg tctggacccc gccgcacttc tgggccctgt cgatgcagta
     2521 ccggacggac tacgcggcgg cggggatccc gatgatgccg gtggtccgcg gcgaggagga
     2581 gacccggcgc cagatcctcc tgtacaccct ggtcctggtg ggcaccagcc tcctgctcta
     2641 cccgctgggc cacatggggc ccgtgtacct ggccgcctcg gtgatcctgg gcgggatgtt
     2701 cctgcaccgg gccttgcggc tgtggaggga gcggagccgg ggcctggcca tcggcctcta
     2761 ccgcttctcc atcctgtacc tggggctgct cttcgccgca gtggcggtgg acgccgtcct
     2821 gcccctggcc cgctgagcgc gctcgctctc cctcgtccct cgtgaggggc cggcggtcgg
     2881 cctagcccga gatccgttgc a
//
LOCUS       DASQQQ010000206         3723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_10835, whole genome shotgun sequence.
ACCESSION   DASQQQ010000206 DASQQQ010000000
VERSION     DASQQQ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10835"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..474)
                     /locus_tag="VE646_07810"
     CDS             complement(205..474)
                     /locus_tag="VE646_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79930.1"
                     /translation="MQHPSERRGPLVRDTGPGPGAGHERDPERAGMLHARIAIVATIV
                     VGQLWGLTVALDRYFLGRSVGWLVVFEALSFAIAVLVWLAAPGEH"
     gene            complement(474..992)
                     /locus_tag="VE646_07815"
     CDS             complement(474..992)
                     /locus_tag="VE646_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZD79931.1"
                     /translation="MSAGGFGEPGRPDGSARVPVRALMDRIREDAPVTRRDYLRILVT
                     ISGGLLAGTIAVSAGVFRRRGAGSAPAKRVARSLPPGASVRFSYPTEQDAAIAMRLAD
                     GTLVGYSSVCTHLSCAVLWNAEGQRLDCPCHDGVFDARDGVPIAGPPPRPLPRVVMRE
                     RPSGIFAIGTEV"
     gene            complement(989..1558)
                     /locus_tag="VE646_07820"
     CDS             complement(989..1558)
                     /locus_tag="VE646_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019766142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZD79932.1"
                     /translation="MMQKTQFLDVGRCIGCQACVAACRECDSHRGRSMIHLDYIDAGQ
                     TVASMPTVCMHCEDPVAPCAEVCPADAILVTADGVVQEAAKERCIGCANCVHACPFGV
                     PKIDIEEKLQYKCNMCYDRTSVGLAPWCATVCPTGAIFYGTLEELLTQRPNATVSDII
                     VFGDQEIRTGVAVVTPAGYAEGAIPGGMA"
     gene            complement(1555..>3723)
                     /locus_tag="VE646_07825"
     CDS             complement(1555..>3723)
                     /locus_tag="VE646_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003968478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HZD79933.1"
                     /translation="HCCFCGVQCGMYLKVDRDGRVFGVEARDHDINHLRLCPKGVVAY
                     QQVNHPDRLTSPLMRDRRGEPLRPVSWDRALDRIVSEIRRIQEEQGRDAFGVIGGASL
                     TNEKAYLVGKFARVALRTRHVDYNGRLCMVSAAAANKKAFGIDRAANPWADLFETQVV
                     IVAGANVGETFPVATQYFWGARDRGAKLIVIDPRETPIARTADVRVRVRPGTDAAFFN
                     GVLNVVVREGLTDERFIDEHTVGWDAVRDAVAAYPPEQVARICGIEPSEVETVAMLWG
                     RAERAMAFHARGLEHHVQGVDNCLSVINLVLATGQLGKPGAGYGTITGQGNGQGGREH
                     GQKSDMLPGMREIENPEHRAHVAAAWGVDEVELPGAGTSFVEMMAQMREGGIRGVIGF
                     CNNPFVSLPNLSTVVQAYDSLEFHAQADFFLSETAARADVVLPSVVWAEDEGTTTNAE
                     GRVVKHNKAAEPPGQARPDWWIMSEIAGRLNRGRWFGFTSARDVFDDLRASSRGGVAD
                     YWGITYEKIEETGGVFWPCPTPEHPGTPRLFEDLRSFFPDGRFRFHAIGWHEPAEPVD
                     EGFPLRLTTGRTVAHFLSGNQTRRIGALVEQTPRPWVEVHPSLGFANGDPVRVVTRRG
                     AVTYPALVTDTIRPDTVFVPYHWSAPVAVNAMTIDALDPVSKIPEFKVCACRIEPGGT
                     IDPVAPPPPPPGRSPTRYEQDTVANLRPPTAPQGRGTSER"
BASE COUNT          574 a         1378 c         1240 g          531 t
ORIGIN      
        1 aacgccacaa ccgacatgag cccgatcgag atcaggccgt tcaggaacga gaacaggccc
       61 aggatgaaca ccgagttgta gcggtcggcc agcgcccgga accggaaccg ccgggccagc
      121 cccaggacga cgtcggacat ccgcccgccg atcgggtacc gctgtgggtc gtcggagggt
      181 cgcgccggct cccggcccgg cacctcagtg ctccccgggc gcggccagcc agaccaggac
      241 ggcgatcgcg aacgacagcg cctcgaacac cacgagccaa cccacgctgc gtcccaggaa
      301 gtagcggtcc agggccaccg tgaggcccca cagctgcccg accacgatgg tggccacgat
      361 cgcgatccgg gcgtggagca tcccggcccg ctccggatca cgctcgtgtc ccgcgcccgg
      421 ccccggaccg gtgtcccgga ccagaggacc gcggcgttcc gacgggtgct gcatcagacc
      481 tccgtcccga tcgcgaagat cccgctcggc cgttcccgca tcaccacccg gggcaggggc
      541 cgcggtggcg ggccggcgat gggcaccccg tcgcgcgcgt cgaacacgcc gtcgtggcac
      601 gggcaatcga gccgctggcc ctccgcgttc cacagcacgg cgcacgacag gtgggtgcac
      661 acggaggagt agcccacgag cgtgccgtcg gccaggcgca tggcgatcgc cgcatcctgc
      721 tccgtggggt aggagaagcg gacggaggcg cccggaggaa gcgaccgtgc cacccgcttg
      781 gccggcgcac tccccgcacc ccggcgccgg aacacgcccg cagacacggc gatcgtgccg
      841 gccagcaggc cccccgagat cgtcaccagg atccgcaggt agtcgcggcg ggtcaccgga
      901 gcgtcctcgc ggatccggtc catcagggcg cgcaccggga cgcgtgccga gccgtcgggg
      961 cggccgggct cgccgaaacc ccccgcgctc acgccatgcc tccggggatc gcgccctccg
     1021 cgtagccggc cggcgtcacc acggcgacgc ccgtccggat ctcctgatca ccgaagacga
     1081 tgatgtcgct cacggtggcg ttcggacgct gggtcaggag ctcctccaag gtcccgtaga
     1141 agatcgctcc ggtggggcag acggtggcgc accaaggagc aagaccgacc gaggtgcggt
     1201 cgtagcacat gttgcacttg tactggagct tctcctcgat atcgatcttg ggcacgccga
     1261 agggacaggc gtgcacgcag ttcgcgcagc cgatgcaccg ctccttggcc gcctcctgca
     1321 ccacgccgtc cgcggtgacc aggatcgcat ccgcagggca cacctcggcg cacggggcca
     1381 ctggatcctc gcagtgcatg cagaccgtgg gcatcgaggc gaccgtttga cccgcatcga
     1441 tgtagtcaag gtggatcatc gaccggcccc ggtgcgagtc gcattcacgg caggcggcca
     1501 cgcaggcctg acacccgatg cagcgaccga cgtccaggaa ctgcgtcttc tgcatcatcg
     1561 ctcgctggtc cccctcccct gcggcgccgt cggcgggcga aggttcgcga ccgtgtcctg
     1621 ctcgtaacgt gtggggctcc ttcctggagg tggcggcgga ggagccaccg gatcgatggt
     1681 ccctcccggc tcgatccggc acgcgcagac cttgaactcg gggatcttgg agacggggtc
     1741 caaggcgtcg atcgtcatgg cgttcacggc gaccggggcg gaccagtgat aggggacgaa
     1801 caccgtgtcg ggtctgatcg tgtccgtcac cagggccggg taggtgaccg cccctcggcg
     1861 ggtcaccacc cggaccgggt cgccgttcgc gaaccccagc gagggatgca cctccaccca
     1921 cggtcgaggg gtctgctcga cgagcgcccc gatccggcgg gtctggttgc ccgagaggaa
     1981 atgggcgacc gtccgtccgg tcgtcagccg gagggggaag ccctcgtcca ccggctccgc
     2041 cggctcgtgc catccgatgg cgtggaaacg gaacctcccg tcggggaaga acgaacgcag
     2101 gtcctcgaac agcctgggcg tgcccggatg ctcgggggtc ggacacggcc agaagacacc
     2161 gccggtctcc tcgatcttct cgtacgtgat gccccagtag tcggccaccc cgcccctcga
     2221 gctggcccgc agatcgtcga acacgtctcg ggcgctcgtg aacccgaacc agcggccacg
     2281 gttcagccgg ccagcgatct cgctcatgat ccaccagtcg ggccgcgcct gccccggcgg
     2341 ctcggcggcc ttgttgtgct tcaccacccg gccctccgcg ttggtggtgg tgccttcgtc
     2401 ctcggcccag accaccgagg gcagcaccac gtcggcccgc gccgccgttt ccgagaggaa
     2461 gaagtcggcc tgcgcgtgga actccaagct gtcgtacgcc tggaccacgg tggacaggtt
     2521 gggcaaggag acgaaggggt tgttgcagaa cccgatcacc ccgcggatcc ccccctcgcg
     2581 catctgggcc atcatctcca cgaacgaggt ccccgctccg ggcagctcga cctcgtccac
     2641 tccccaggcg gccgcgacgt gcgcgcgatg ctccgggttc tcgatctcgc gcatgcccgg
     2701 gagcatgtcg gacttctgac cgtgctcccg accgccctga ccattgccct gcccggtgat
     2761 cgtgccgtac ccggcgcccg gcttccccag ctgtcccgtg gccaggacca ggttgatgac
     2821 cgacaggcag ttgtccaccc cctgcacgtg gtgctccaag ccccgcgcgt ggaaggccat
     2881 ggcgcgctcc gcccggcccc agagcatggc caccgtctcc acctcggagg gctcgatccc
     2941 gcagatccgt gccacctgct cgggcgggta ggccgccacc gcgtcccgca ccgcgtccca
     3001 tcccaccgtg tgctcgtcga tgaaccgctc gtccgtcaga ccctcccgca cgacgacgtt
     3061 cagcacgccg ttgaagaacg ccgcgtcggt gccgggacgg acccgcaccc gcacgtccgc
     3121 ggtgcgggcg atgggggtct cccgtgggtc gatcacgatc agcttggccc cccggtcccg
     3181 agcgccccag aagtactggg tggccacggg gaaggtctcc cccacgttgg ccccggccac
     3241 gatgaccacc tgggtctcga acagatccgc ccatgggttc gccgcgcggt cgatgccgaa
     3301 cgccttcttg ttcgcggccg cggccgacac catgcagaga cgcccgttgt agtccacgtg
     3361 ccgggtgcgg agggcgaccc gggcgaactt gccgaccagg taggccttct cgttcgtcag
     3421 gctggccccg ccgatcaccc cgaaggcgtc ccgtccctgc tcctcctgga tccgccggat
     3481 ctcggacacg atccggtcga gggctcggtc ccaggagacc ggccggagag gctcacctcg
     3541 ccgatcgcgc atcaacgggc tggtgagacg gtcggggtgg ttcacctgct ggtaggcgac
     3601 cacgcccttg gggcacagcc ggaggtggtt gatgtcgtga tcgcgggctt ccaccccgaa
     3661 cacccggccg tcccggtcca ccttcaggta catcccgcac tgcacgccgc agaagcagca
     3721 gtg
//
LOCUS       DASQQQ010000207         7938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_229488, whole genome shotgun sequence.
ACCESSION   DASQQQ010000207 DASQQQ010000000
VERSION     DASQQQ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229488"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1874)
                     /gene="gyrB"
                     /locus_tag="VE646_07830"
     CDS             complement(<1..1874)
                     /gene="gyrB"
                     /locus_tag="VE646_07830"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HZD79934.1"
                     /translation="MATRKTHNSTSGDRGYTSADITVLEGVEHVRRRPGMYIGTTGPR
                     GLHHLVYEVVDNSVDEAMAGFCDRIVVTLLKDGGCRVTDYGRGIPVKPIPKAKDRRPA
                     VEVVLTTLNAGGKFGGRGYRISGGLHGVGVSVVNALSARMVVQVERDGATWTQSYEQG
                     KATSKLVKGKPSRRSGTTITFWPDADIFEEVAFKRDILAERLQELAFLTRGLEIQLID
                     EREDPPIKEVFKASGGLADFVKKLQSGKETLHNRTIHFEGQESDGSAGAEIALQWSAG
                     YSESIHSFANTINTHEGGTHEEGLKKALTNVVNRYARAKNLLKEKEDNLQGEDVREGL
                     VGIVSVKLREPQFEGQTKTKLGNTSIRSLVETTVNEHLATWLEEHPGDAKRIVLKGSA
                     AAKARMAAKHARDLTRRKSFLEGGGLPGKLADCQLSDPSACELYIVEGDSAGGSAKSA
                     RDRATQAILPIRGKILNVEKARLHKILENNEIQALITAIGTGISDEFDLSKARYHRIV
                     IMADADVDGAHIRTLLLTFFFRNMRELIDAGYVYLAQPPLYLLKEGKQERYFYTEREW
                     EQFRGQDGNGERKLDPQRFKGLGEMDAGQLWDTTMDPERRTLLRVTLEDAATADRLFT
                     V"
     gene            complement(2014..2367)
                     /locus_tag="VE646_07835"
     CDS             complement(2014..2367)
                     /locus_tag="VE646_07835"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HZD79935.1"
                     /translation="MPSSKGWRGRNDERTLRETPIGDIVDGLLQERPFARGMPIGRLA
                     GSWTEIVGPRLASQTAPVSLERGVLVVAASTGPWGAQARFLASAIRERADLALGGDQV
                     RTVHVVVRPERRKGL"
     gene            complement(2367..3479)
                     /gene="recF"
                     /locus_tag="VE646_07840"
     CDS             complement(2367..3479)
                     /gene="recF"
                     /locus_tag="VE646_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HZD79936.1"
                     /translation="MRLRWLELLDLRNHERTRLDQIPAALITVVGPNGEGKSNLLEGI
                     AFLFFLASPRISTTQPLVREGKDAAYARGEVETSTGRLLVEVEIRSRGADRVQANRSP
                     VRRKRDVRRQIRAVFFGPDDLDVVRGDPSQRRRFMDEALTSLWPLRGSAITAYERTLR
                     QRNRLLKDWGGGGSPPGLQAWDEELVANGCALMRLRSEAVARLAPGAGEEFRDLSGYG
                     LGCRYRPNVPGTGDQITADPLEAAFWARLEERRGDELVRRTSLVGPHRDDLELAVRDL
                     GARSFASHGEAWAAALCLRLGLSSAIEAEIGEHPVLLLDDPFSALDPRRQAQVGARLA
                     ERGQVLISVADEAHVPQRTQAVWDVSAGHVTVRGVA"
     gene            complement(3496..4599)
                     /gene="dnaN"
                     /locus_tag="VE646_07845"
     CDS             complement(3496..4599)
                     /gene="dnaN"
                     /locus_tag="VE646_07845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HZD79937.1"
                     /translation="MKFRCDRDALSEALQIVQRGVSSRPGIPALTGVLVEAGEGGALT
                     LTTTDLEVSARLSIEVQVQDAGIALVPARLLADTVKSLSDAPVEFESDQGQAQIRCAA
                     YEGALRLLPAEDFPGLQPPSGTRVVVEATRFAEAVGQVARAASRDEARPVLTGVLVEV
                     SREGLTLVATDSYRLAVRELIATAEGEAKAIVPERAMAEAGRAAAGIEKGEVELFVDE
                     SQVSFRAGKLMLTSRLIEGEFPNYRQLLPEAYENRLTVSRQQFVDAVRRVGLLARDTS
                     PVRMEFNALGVKLSSSSPDLGQAVEAVEAGYEGEEITAAFNATYLADGLAAAAGDTVR
                     LEVRDGLKPGIVRGEGDEFTYLVMPIRLPTPVG"
     gene            complement(4800..6179)
                     /gene="dnaA"
                     /locus_tag="VE646_07850"
     CDS             complement(4800..6179)
                     /gene="dnaA"
                     /locus_tag="VE646_07850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HZD79938.1"
                     /translation="MTVTSFEEAADRTWSRVADRARRELSESSFAMWFSGLRATSLHD
                     GVLEVSAPSDYVRDWLAKHYLTMIESAASDAFGSPLQIVLDAREEPAERPTIADPPSP
                     TPTLVRTGSVTLGVPGLPFPGYTFEAFVAGPSNRFAHAAAMAVAEAPPSKAYNPLFIY
                     GGVGLGKTHLIVAIGHHMHRLAPRLRVKYVTSESFMAEFIKAVRERQGYQFAARYRDI
                     DVLLVDDIQFLAKREETQTEFFHTFNALHEKERQIVIASDRPPQELGMEERLQSRFRL
                     GLCVDVQPPDLETRIAILQLKAQREAVHVPDDVIEFVASKFDQNIRELEGALVRVVAW
                     SDLTGHPIGMELAEHALEDLLPQAEAEIPPQLIVEETAKYFSLTVDELVSKSRSRPLT
                     TARHVAMYLVRECTGMTLPKIGELFDRDHTTALHGINKIDGSMRNREPVYRQVQDLSR
                     IIRSRARNL"
     gene            6434..6571
                     /gene="rpmH"
                     /locus_tag="VE646_07855"
     CDS             6434..6571
                     /gene="rpmH"
                     /locus_tag="VE646_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HZD79939.1"
                     /translation="MSKRTFQPNNRRRKKTHGFRTRMRTRAGRAILGRRRAKGRSRLS
                     A"
     gene            6612..6896
                     /locus_tag="VE646_07860"
     CDS             6612..6896
                     /locus_tag="VE646_07860"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_function: GO:0042781 - 3'-tRNA processing
                     endoribonuclease activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HZD79940.1"
                     /translation="MLSQGRPVHGTRMVVFVAPGPGSVAFVAGRRVGGAVVRNRARRV
                     MRAAWRELVPRAREGYDVVLVARAAIRGAKTQDLVAEMQELLAHARVIDP"
     gene            6893..7204
                     /gene="yidD"
                     /locus_tag="VE646_07865"
     CDS             6893..7204
                     /gene="yidD"
                     /locus_tag="VE646_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005769988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZD79941.1"
                     /translation="MKALRRVVWAAGAPARLALVGLIRVYRLLFSGWLGGQCRFYPSC
                     SHYAEEAVRVHGAIKGSGLAIWRILRCGPFTDGGVDHVPARRGASYDDVIQSHPEVKA
                     T"
     gene            7201..>7938
                     /locus_tag="VE646_07870"
     CDS             7201..>7938
                     /locus_tag="VE646_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HZD79942.1"
                     /translation="MIAAVPWSPAWQGLLYGFGWMLARVYDLIPNFGLAIIVLTVLIR
                     VVLLPLGIRQVRSMQHMQIIQPKVKQIQQKFKGNRAKQQEEIMKLYKEYGVNPFSGCW
                     PMLIQFPILIAMYAVVRFPQHPIHVPQDSRLYQAVERQIPPTQPPKTAQPDNGTDFLW
                     MNMLCSPLQAGNPEATLGAVGANGQKQEYPVDCGNSIPYRIPYYIFAGLMFVTTYWQQ
                     RQMQQASPPGAASQQQQTLLKVMPVMFG"
BASE COUNT         1190 a         2810 c         2635 g         1303 t
ORIGIN      
        1 accgtgaaca ggcggtcggc cgtggccgcg tcctccagcg tgacccgcaa gagcgtgcgc
       61 cgctccgggt ccatggtggt atcccatagc tgtcccgcgt ccatctcacc aagacccttg
      121 aaccgctgcg ggtcgagctt cctctcgccg ttgccgtcct ggccacggaa ctgctcccac
      181 tcccgctccg tgtagaagta gcgctcctgc ttgccctcct tcagcaggta cagcggcggc
      241 tgggccaggt agacgtaccc ggcgtcgatc agctcgcgca tgttccggaa gaagaacgtg
      301 agcagcaggg tccggatgtg ggcgccgtcg acgtcggcgt ccgccatgat cacgatgcgg
      361 tggtaccgag ccttggagag gtcgaactcg tcgctgatgc cggtgccgat cgccgtgatc
      421 aacgcctgga tctcgttgtt ctcgaggatc ttgtgcagcc gggccttctc cacgttcaag
      481 atcttgcccc ggatcggcag gatggcctgg gtggcccggt cccgggcgct cttggccgag
      541 ccacccgcgc tgtcgccctc gacgatgtag agctcgcagg cggacgggtc gctcagctgg
      601 cagtccgcca gcttgccggg cagccccccg ccctcgagga aggacttgcg gcgggtgagg
      661 tcgcgcgcgt gcttggcggc catacgcgcc ttggcggcgg ccgagccctt cagcacgatc
      721 cgcttggcgt cgccgggatg ctcctcgagc caggtggcca gatgctcgtt gaccgtggtc
      781 tccaccaagg agcggatcga ggtgttcccg agtttggtct tggtctgccc ctcgaactgg
      841 ggctcccgaa gcttgaccga cacgatgccg accagtccct cccggacgtc ctcgccctgc
      901 aggttgtcct ccttctcctt gaggaggttc ttggcccggg cgtagcggtt caccacgttg
      961 gtgagggcct tcttgagccc ctcctcgtgg gtcccgcctt cgtgggtgtt gatcgtgttg
     1021 gcgaaggagt ggatcgattc cgagtacccc gcgctccact gcagggcgat ctccgccccc
     1081 gccgatccgt cggactcctg tccctcgaag tggatggtgc ggttgtgcag ggtctccttg
     1141 cccgactgga gcttcttcac gaagtccgcc agcccaccgg aggccttgaa cacctccttg
     1201 atcggagggt cctcccgctc gtcgatcagc tggatctcga gcccgcgggt gaggaaggcc
     1261 agctcctgga gccgctctgc gaggatgtcc cgcttgaagg cgacctcctc gaagatgtcg
     1321 gcgtccggcc agaacgtgat cgtggtaccg gatcgccggc tgggcttgcc cttgaccagc
     1381 ttgctggtgg ccttgccctg ctcgtagctc tgggtccagg ttgccccatc gcgctccacc
     1441 tggaccacca tccgagccga cagggcgttc accaccgaca ccccgacccc atgcaagcct
     1501 cccgagatgc ggtacccgcg gccgccgaac ttgcctccgg cgttcaaggt agtcagcacc
     1561 acctccaccg ccggccgacg gtccttggcc ttggggatgg gcttgaccgg gatgccccgg
     1621 ccgtagtccg tgacgcggca ccccccatcc ttcagcagcg tcacgacgat ccggtcacag
     1681 aaccccgcca tggcctcgtc gaccgagttg tcgacgacct cgtacacgag gtggtggagc
     1741 ccccgggggc cggtggtccc gatgtacatc cccgggcgcc ggcggacatg ctcgacgccc
     1801 tccaggacgg tgatgtcggc ggaggtgtat ccccggtctc cactggtgct gttatgtgtc
     1861 ttacgagttg ccaacgaacc tccggatcca tgcctcgcgc agggcaccat agggccggtg
     1921 cgctgagggg gcctcacgcg gggaacgacc cctatatcct accaatctgg acccccctcc
     1981 gccctgcccg cagggatcaa agatatcctt gcgctacaag cctttccgcc gttccggccg
     2041 caccaccacg tgcaccgttc ggacctggtc cccaccgagg gccagatcgg cccgttcccg
     2101 gatggccgag gccaggaagc gggcctgggc gccccacggc cccgtgctcg ccgcgaccac
     2161 caacaccccc cgctccaagc tcaccggcgc ggtctgtgag gccaggcggg gccccacgat
     2221 ctccgtccag gagccggcca gccgaccgat gggcatccca cgcgcgaacg ggcgctcctg
     2281 gagcagcccg tcgacgatgt cgcctatggg cgtctcccgg agggtgcgct cgtcgttccg
     2341 tccccgccat cccttgctcg agggcatcag gccacgcccc ggaccgtaac gtgccccgcc
     2401 gacacgtccc acacggcctg ggtccgctgg ggcacgtgag cctcgtcggc caccgagatc
     2461 aggacctgtc cccgttcggc caggcgcgcg ccaacctggg cctgtcgccg gggatcgagc
     2521 gccgagaacg ggtcgtccag cagcagcacc gggtgctcgc cgatctcggc ctcgatcgcc
     2581 gacgataggc ccagccgtag gcacagagcc gcggcccacg cctcgccgtg cgaggcgaag
     2641 ctccgagcgc ccaggtcccg caccgcgagc tcgaggtcgt cgcgatgggg acccaccagg
     2701 ctggtccgcc ggacgagctc gtcaccccgg cgctcctcca gacgagccca gaacgccgcc
     2761 tccaggggat cggcggtgat ctggtccccg gtccccggca cgttgggccg gtaccggcag
     2821 cccaaaccgt accccgagag gtcccggaac tcctccccgg ccccaggcgc cagccgcgcc
     2881 acggcctcgg agcgcagccg catgagcgcg cacccgttcg ccaccaactc ctcatcccag
     2941 gcctggaggc cgggtggcga tccaccgccg ccccagtcct tcagcaggcg gttccgctga
     3001 cgaagggtgc gctcgtaggc cgtgatggcg gacccgcgca ggggccacag cgaggtcagc
     3061 gcctcatcca tgaaccggcg ccgctgcgag ggatcccccc tcaccacgtc gaggtcgtcg
     3121 gggccgaaga acacggcccg gatctgccgg cggacgtccc gcttgcgtcg cacgggtgac
     3181 cggttcgcct gcacgcgatc ggccccccgg ctccggatct ctacctccac cagcaggcgg
     3241 cccgtcgagg tctcgacctc cccccgcgcg taggcggcat ccttcccctc gcgcaccaag
     3301 ggctgggtcg tcgagatgcg gggcgaggcc aggaagaaca ggaacgcgat gccctcgagc
     3361 aggttggact tcccctcccc gttcggaccc accaccgtga tcagggcggc cgggatctgg
     3421 tccagccgcg tccgctcgtg gttgcggaga tccaggagct cgagccaccg gagccgcacc
     3481 gcgttcccgg cgcgatcagc cgaccggcgt gggcaggcga atcggcatca ccagataggt
     3541 gaactcgtcg ccctcgcccc gcacgatccc gggcttcaag ccgtcgcgca cctcgagccg
     3601 gaccgtgtcc ccagccgccg ccgcgagccc gtccgccagg tacgtagcgt tgaacgccgc
     3661 ggtgatctcc tccccctcgt agccggcctc gacggcctcg acggcctgcc ccagatccgg
     3721 cgaggaagag gagagcttga cccctagcgc gttgaactcc atgcgcaccg gcgaggtgtc
     3781 ccgcgccagc agccccaccc gtcgcaccgc gtccacgaac tgctgcctgg ataccgtgag
     3841 ccggttctcg taggcctccg gcagcagctg tcggtagttg ggaaactcgc cctcgatcag
     3901 ccgcgacgtc agcatcagct tcccggcccg gaagctcacc tggctctcgt ccacgaacag
     3961 ctctacctcg cccttctcga tcccggcggc cgcccggccg gcctcggcca tggcgcgctc
     4021 ggggacgatg gccttggcct ctccctcggc cgtggcgatc agctctcgga ccgccaagcg
     4081 gtacgagtcc gtggccacga gggtcaggcc ttcccgactc acctcgacca ggacgcccgt
     4141 gagcaccggg cgggcctcgt cgcgggatgc cgcccgggcc acctggccca ccgcctccgc
     4201 gaacctcgtg gcctccacca ccacccgtgt gcccgaggga ggctgcaggc ccgggaagtc
     4261 ctcggcgggg agcagccgga gggcaccctc gtacgccgcg caccggatct gggcctggcc
     4321 ctgatcggac tcgaactcca ccggggcgtc cgagaggctc ttcacggtgt ccgccaggag
     4381 ccgcgccggc accagcgcga tgccggcatc ttggacctgg acctcgatcg acagccgtgc
     4441 cgagacctcc aggtccgtcg tggtgagggt gagggcgccg ccttcccccg cctcgaccag
     4501 cacgcccgtg agggcgggga tccccggccg ggacgacacg ccgcgttgca cgatctgaag
     4561 cgcctcggac agcgcatctc ggtcacaccg gaacttcacc tgtccacccc ctcgctgctt
     4621 ctacggttcc cagaacttcc agatcagtgg tagtagagcc tggggaaagg tggataagcc
     4681 ctccatcgcc ccagcgtatc gggtccgtgc gacatgggga cggcgtgtgg aacggagcgg
     4741 gcaaccccgg tgaggagcca cggcccacac aggggttccc acgatgtcca cagggttgct
     4801 cacaggttcc tcgcgcggct gcggatgatc cgggacaggt cttggacctg gcggtagacg
     4861 ggctcgcggt tgcgcatgct gccgtcgatc ttgttgatcc cgtggagggc ggtggtgtgg
     4921 tctcggtcga agagctcacc gatcttgggc agggtcatgc cggtgcattc gcgcaccagg
     4981 tacatcgcga cgtgcctggc cgtggtgagt ggccgggagc ggctcttgga caccagttcg
     5041 tccaccgtga ggctgaagta cttagccgtc tcctccacga tgagctgcgg cgggatctcg
     5101 gcctccgcct gcgggaggag gtcctcgagg gcgtgctcgg cgagctccat gccgatgggg
     5161 tggccggtca gatcgctcca cgcgaccacc cggaccaggg cgccctccaa ctcgcggatg
     5221 ttctggtcga acttgctggc aacgaactcg atcacatcat cggggacatg caccgcctcg
     5281 cgctgggcct tgagctgcag gatcgcgatc cgggtctcga ggtcgggcgg ctggacgtcg
     5341 acgcacaagc ccaaccggaa gcggctctgt aaccgctcct ccatccccag ctcttgtggt
     5401 ggccggtcgg acgcgatgac gatctgccgc tccttctcgt gtagcgcgtt gaaggtatgg
     5461 aagaactcgg tctgcgtctc ctcccgcttg gccaggaact ggatgtcgtc cacgagcagc
     5521 acgtcgatgt cccggtagcg agcggcgaac tggtagcctt ggcgctctcg gacggccttg
     5581 atgaactcgg ccatgaagct ctcgctggtc acgtacttca cccggagccg gggagcgagc
     5641 cgatgcatgt ggtggccgat cgccacgatc aggtgtgtct tgccgaggcc gacgccgccg
     5701 tagatgaaca gcgggttgta ggccttggac ggaggcgcct cggcgacggc catcgcggcg
     5761 gcatgggcga accggttgct cggccccgcc acgaaggctt cgaaggtgta cccggggaac
     5821 ggcaggccgg gcaccccgag cgtcaccgag ccggtccgca cgagcgtggg ggtgggcgat
     5881 ggtggatcgg cgatcgtcgg gcgctccgcc ggttcctccc tggcgtccag gacgatctgc
     5941 aagggtgagc cgaacgcatc cgatgcggcc gactcgatca tcgtcaggta atgcttggcg
     6001 agccagtcac ggacgtagtc ggagggggcg ctgacctcca ggacgccatc gtggaggctg
     6061 gtggcccgca ggccggagaa ccacatggcg aacgaactct cggagagctc gcgccgggcc
     6121 cgatcggcca cacgggacca ggttcgatcg gccgcttctt cgaaggatgt caccgtcatg
     6181 accgattccc acaacacctt tccacacgtg tggaaaccct gggggaaggg aaacggcccc
     6241 gcggtccgga tcccgggggg gccgccaagg ggaaggcgcc gcggactata gcacccgggt
     6301 tttccccacg gggcgggggg cggggccttt tggcgcactt tctccccacc cgtccctttg
     6361 tccggccccg acggggtccc gtatactctg ccggccggcc gctgggccgg ctttctcgac
     6421 ctcgtgaggc acgatgtcca agcgaacgtt ccagccgaac aaccgtcgcc ggaagaagac
     6481 gcacgggttc cgtacgcgca tgcggacccg tgctggccgt gcgatcctgg gccgccggcg
     6541 ggccaagggc cggagccggc tatcggcctg atcgggtcgg agccatcggc acctcgtccg
     6601 gtgtcggcag ggtgctctcc cagggcaggc ctgtccacgg aacccggatg gtagtgtttg
     6661 tcgcgcccgg cccgggcagc gtcgcgttcg tcgccggccg gcgagtcggg ggggccgtcg
     6721 tccgtaaccg ggctcggcgg gtcatgcggg ccgcctggcg ggaactggtt ccccgggcgc
     6781 gggagggata cgacgtggtc ctggtggccc gggcggccat ccgaggggcc aagacacagg
     6841 atctggtagc cgagatgcag gaacttctcg cgcatgcgcg ggtgatcgac ccgtgaaggc
     6901 gctccggcgg gtggtgtggg ccgcgggggc ccccgcaagg ctcgcgctgg tggggctgat
     6961 tcgtgtctac cggctgctgt tctcgggctg gctgggtggg caatgccgct tctacccgag
     7021 ttgctcccac tacgcggagg aggccgtgcg ggtgcacggc gcgatcaagg ggtccggtct
     7081 ggcgatctgg cggatcctcc ggtgcggccc gttcaccgat ggaggggtcg accacgtccc
     7141 tgctcgccgc ggtgcttcgt atgatgatgt catacagtcc caccctgagg tgaaggccac
     7201 gtgatcgccg ccgtcccctg gtcgcccgcc tggcaggggt tgctgtacgg cttcggctgg
     7261 atgctggcgc gcgtctacga cctcatcccc aacttcggct tggccatcat cgtcctcacg
     7321 gtactgatcc gggtggtcct cctgccgcta gggatccgac aggtccggtc gatgcagcac
     7381 atgcagatca tccagccgaa ggtgaagcag atccagcaga agttcaaggg caaccgcgcc
     7441 aagcagcagg aagagatcat gaagctgtac aaggagtatg gggtgaaccc gttctccggg
     7501 tgctggccga tgcttatcca gttcccgatc ctgatcgcga tgtacgcggt ggtccgcttc
     7561 ccgcagcacc cgatccatgt tccccaggac agccggctgt accaggcggt cgagcgccag
     7621 atcccaccca cccagccgcc gaagacggct caacccgaca acggcaccga ctttctctgg
     7681 atgaacatgc tctgctcacc actgcaggca ggcaaccccg aggcgacgtt gggggcggtc
     7741 ggcgccaacg gccagaagca ggagtatccg gtcgactgcg gtaactcgat cccctatcgg
     7801 atcccctact acatcttcgc ggggctcatg ttcgtcacca cctactggca gcaacgtcag
     7861 atgcagcagg ccagtccccc cggggccgcc tcgcagcagc agcagaccct gctgaaggtg
     7921 atgccggtca tgttcggc
//
LOCUS       DASQQQ010000208         5590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_154489, whole genome shotgun sequence.
ACCESSION   DASQQQ010000208 DASQQQ010000000
VERSION     DASQQQ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154489"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="VE646_07875"
     CDS             complement(<1..1179)
                     /locus_tag="VE646_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD79943.1"
                     /translation="MSAAVPLDIAEPGASRRALAVGAFFLIAAAVILVVFALGVPSGE
                     SSTFVLNPVGGGPIHIPDLTVPSRATSLILGVLCAGLGAYQLVRGFGERTNLVLGIVV
                     TLFVVAFLAWAAHGKSFNLVGMFQSTLLLAVPITFGALSGVLCERAAVINVGIEGMLL
                     AGAFAGTVVGSVAGGASGVHLVGGTPAEWLGLAAGAVVGGLLGALLAVLSIRYVVDQI
                     IAGVAINIVALGLTSYLSVAFLEQNNALNAPGIFRGVAIPGLAKLPVIGPVLFDQNIF
                     VYLMLIFVALIYVGLFHTRWGLRVRAVGEHPRAADTVGIRVLAIRYWNVILGGLVAGI
                     GGAFFTLGSVGRFDENMTAGRGFIGLAAMIFGRWNPVGGFLAALVFGFADALSTKLSI
                     L"
     gene            complement(1176..2489)
                     /locus_tag="VE646_07880"
     CDS             complement(1176..2489)
                     /locus_tag="VE646_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014987371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD79944.1"
                     /translation="MADIKQGRPPTEEEEEPRASGEETAGASRSFASDVLEGGAGPSW
                     LRALSIPVLAILTALVLGALLIVFTDSTSLHAWASFFRDPIGALSASWTAVREAYQAL
                     FTGALGSPSEIAGAIGSGDLHRITLSLNPLSETVIASTPLIFAGLSVALGFRAGLFNI
                     GAEGQINVGAIAAASVGFSFAGLPGPLHLTLIVGAGFLGGALWGGIPGYLKAKTGAHE
                     VITTIMMNYISYYLVDYLLLSSFFQRPGRTDPISKPVTAFFPHLLGQDLRLNAGIFVA
                     LLMAIVTAWLLNRSTVGFQFKAVGANPDAARTAGMSPGRTFVWAMALSGALAGLGGAV
                     QLASVSPSLTPGFSSGYGFDAIALALLGKAKPSGVVAAAFLLGALRAGARSMQAATST
                     PIDIVTVIEALVIVFVAAPAVVRGIYRIKAQRAEGPQVLTKGWGA"
     gene            complement(2482..4023)
                     /locus_tag="VE646_07885"
     CDS             complement(2482..4023)
                     /locus_tag="VE646_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018347921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD79945.1"
                     /translation="MRLELKGISKRFPGVVANDRISLAVEPGEIHALLGENGAGKTTL
                     MNILYGLYHADEGEILIDGDPVRFDSPGDAIAAGLGMVHQHFMLVPVFSVTENIMLGL
                     ESTKGRLGLLDRAVARRHVERISREFGLEVPPDALIEELPVGVQQRVEIVKALYREAQ
                     VLILDEPTAVLTPQETEELFRVMRSLKAAGKSIIFITHKLKEVLAIADQITVLRRGGV
                     VGTTRPEETTEPELAAMMVGRAVMLEVAKDPAEPGEPVLEVGDLVVRDDRGHIAVDGV
                     SLQVRAGEIVCVAGVQGNGQTELVEALTGLRPAAAGDIRILGHGMMDASVHRVLDQGV
                     GHVPEDRLRDGLVVDFSIADNLVLDHYDRPPFAHWIVRNEREVLDVAKERAQSFDIRT
                     PSVLQPASTLSGGNQQKVIVAREFSRPIRLLIASQPTRGLDVGSIEYIHGRLVAKRDE
                     GVAVLIVSPELDEVVALADRIAVMYRGRIVAVLPAGAASREQLGLLMAGVAPDEVLDE
                     EAVGG"
     gene            complement(4124..5206)
                     /locus_tag="VE646_07890"
     CDS             complement(4124..5206)
                     /locus_tag="VE646_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HZD79946.1"
                     /translation="MNRIFRSVAVLAVLAMLAAACASNNESGGNGGSTSASEQPGAGV
                     TACEVTDTGGINDKSFNATAWKGVTDAEKDYGVAGKYLESTTQDDYKPNIQAFLGKCD
                     LIVTVGFLLGDATEAAAKANPDQNFAIVDYAYPKTIPNVLALTFSTDQAAFLAGYLAA
                     GMTKTGKVGTFGGINIPTVSIFMNGYAAGVLKYNQDNGTNVQVLGWNPAKQDGTFTGD
                     FEDQNKGRQVTESLLSEGADIIMPVAGPVGLGAAAAVQASGNAMLIGVDTDWFVSAPE
                     YQDIELTSVQKNMDVAVEDAVKTVIDGSFQGGTYVGTLENDGVGIAPFHNFDSQVPSD
                     LKSKIDELKQGIIDGSISVDPKDYLS"
     gene            complement(5378..>5590)
                     /locus_tag="VE646_07895"
     CDS             complement(5378..>5590)
                     /locus_tag="VE646_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HZD79947.1"
                     /translation="GSAACHSVREGDPASMRFVCVVSAPEGLAKMQQEHPDVPIFTAA
                     VDRQLNERGYILPGLGDFGDRLFGTE"
BASE COUNT          883 a         1971 c         1919 g          817 t
ORIGIN      
        1 caggatcgac agtttcgtgg acagggcgtc ggcgaacccg aacacgagcg ccgccaggaa
       61 cccgccgacc gggttccacc gcccgaagat catggcggcc aggccgatga acccgcggcc
      121 cgcggtcatg ttctcgtcga accgtcccac cgagccgagc gtgaagaacg caccaccgat
      181 gccggccacc agcccgccca ggatcacgtt ccagtacctg atggccagga cccggatgcc
      241 cacggtgtcg gccgcgcgcg ggtgctcgcc gaccgcgcgc acccgcaacc cccaccgcgt
      301 gtggaacagg cccacgtaga tcagcgcgac gaagatcagc atcaggtaca cgaagatgtt
      361 ctggtcgaag agcacggggc cgatcacggg cagcttggcc agacccggga tggccacgcc
      421 ccggaagatc ccgggggcgt tcagcgcgtt gttctgctcg aggaaggcca cggacaggta
      481 gctggtcagg cccagggcca cgatgttgat ggccacgccc gcgatgatct ggtccaccac
      541 gtagcggatc gacaggacgg ccagcagcgc ccccagcagg cccccgacca cggcgccggc
      601 ggcgaggccg agccactcgg cgggtgtccc gcccaccagg tgcacccccg aggcgccgcc
      661 cgccacgctg cccaccaccg tccccgcgaa cgccccggcc agcagcatcc cctcgatgcc
      721 cacgttgatc accgcggcgc gttcgcacag cacgcccgag agggcgccga aggtgatcgg
      781 cacggcgagc agcaacgtgc tctggaacat ccccacgagg ttgaaggact tgccgtgggc
      841 cgcccaggcc aggaacgcca ccacgaacag cgtgaccacg atgccgagca ccaggttggt
      901 ccgctccccg aagccgcgga ccagctggta cgcgccgagc ccggcgcaca ggacgccgag
      961 gatcagtgat gtggcgcggg agggaacggt gaggtccggt atatggatcg gcccgccccc
     1021 cacggggttc aggacgaacg tggaggactc accgctcggc acccccaggg cgaacaccac
     1081 gaggatcacc gcggcggcga tgaggaagaa cgcccccacg gcgagggctc gacgggaggc
     1141 gccgggctcg gcgatgtcca gcgggacggc ggcgctcacg ctccccaccc cttggtcagc
     1201 acctgcggcc cctcggcacg ttgggccttg atgcgataga tgccccgcac cacggccggc
     1261 gcggcgacga acacgatcac cagagcctcg atgaccgtga cgatgtcgat gggcgtgctg
     1321 gtggccgcct gcatggaccg ggctccggca cgcagggcgc ccagcaggaa cgccgcggcc
     1381 accacgccgg agggcttggc cttgcccagc agggccagcg cgatggcgtc gaacccgtac
     1441 cccgaggaga agcccggcgt caggctgggc gagacgctgg ccagctgcac cgcgcccccg
     1501 aggccggcca gggcgccgga gagggccatg gcccagacga acgtcctgcc ggggctcatc
     1561 cccgccgtgc gggcggcgtc gggattggcc ccgaccgcct tgaactggaa cccgacggtc
     1621 gaccggttca gcagccacgc ggtcacgatc gccatgagca gggccacgaa gatcccggcg
     1681 ttcagccgca ggtcctgccc gagcaggtga gggaagaacg cggtcacggg cttggagatc
     1741 gggtccgtcc tgcccggccg ctggaagaag gacgagagca ggaggtagtc cacgaggtag
     1801 taggagatgt aattcatcat gatcgtggtg atgacctcgt gggccccggt cttggccttc
     1861 aggtagccgg ggatgccgcc ccacagggcc ccgcccagga accccgcgcc gacgatcagc
     1921 gtgaggtgga gcggtccggg gaggccggcg aacgagaagc ccacggaggc ggccgcgatc
     1981 gcgccgacgt tgatctgtcc ctcggcgccg atgttgaaca ggccggcccg gaagccgagc
     2041 gctaccgaca gaccggcgaa gatcagcggg gtcgaggcga tcaccgtctc cgagagcggg
     2101 ttcaacgaca gggtgatccg atgcaggtcg ccgctcccga tcgcaccggc gatctccgag
     2161 gggctcccca acgccccggt gaacagcgcc tggtacgcct ctcgcacggc ggtccaggag
     2221 gccgagagcg ccccgatcgg atcgcggaag aagctggccc acgcgtggag cgatgtggag
     2281 tccgtgaaca cgatcaggag ggcgccgagc acgagcgccg tgaggatcgc cagcacgggg
     2341 atggacaggg cgcgcagcca ggaggggccg gcgccgccct cgagcacatc ggaggcgaac
     2401 gatcgggagg ctccggccgt ctcctcgccg gaggcgcggg gctcttcctc ctcctcggtg
     2461 ggagggcgtc cctgcttgat gtcagccacc gacggcctct tcgtccagga cctcgtccgg
     2521 cgccaccccc gccatcagca ggccgagctg ctcgcgggac gcggcgccgg ccggcagcac
     2581 ggccacgatc cgccctcggt acatgacggc gatccggtcg gccagggcca ccacctcgtc
     2641 gagctcgggg gagacgatca ggacggcgac gccctcgtca cgcttggcca ccaggcgccc
     2701 gtggatgtac tcgatcgagc ccacgtccag ccctcgggtg ggctggctgg cgatcaagag
     2761 tcggatcggt cgggagaact cgcgggccac gatcaccttc tgctggttcc cgcccgacag
     2821 ggtcgaggcc ggctgcagca ccgagggcgt gcggatgtcg aagctctgcg cgcgctcctt
     2881 ggccacatcg aggacctcgc gctcgttccg gacgatccag tgcgcgaacg gtggacgatc
     2941 gtagtgatcg aggaccaggt tgtcggcgat gctgaagtcc accaccaacc cgtcgcgcag
     3001 ccggtcctcg gggacgtggc cgaccccttg gtcgagcacg cgatggaccg aggcatccat
     3061 catgccgtgg cccaggatcc tgatgtctcc ggccgcggcc ggccggagcc ccgtaagggc
     3121 ctccaccagc tcggtctgtc cattgccctg gacaccggcc acgcagacga tctctcccgc
     3181 ccggacctgc aggctgacgc cgtccacggc gatgtgccct cggtcgtccc gaaccaccag
     3241 gtcgcccact tccagcaccg gttctccggg ctcggccggg tccttggcca cctcgagcat
     3301 caccgctcgg cccaccatca tggcggccag ctcgggttcg gtggtctcct ccggcctggt
     3361 ggtccccacc accccacccc ggcggagcac ggtgatctgg tccgcgatgg ccaggacctc
     3421 cttcagcttg tgggtgatga agatgatcga cttgcccgcc gccttgaggg accgcatcac
     3481 ccggaacagc tcctcggtct cctgcggcgt gaggacggcg gtgggctcat ccaggatcag
     3541 gacctgtgcc tcgcggtaga gggccttcac gatctccacc cgctgctgga cccctaccgg
     3601 gagctcctcg atcagggcgt ccgggggcac ctccaggccg aactcgcggc tgatccgctc
     3661 cacgtggcgc cgggccaccg cgcggtccag cagacccagc cgccccttgg tggactccag
     3721 gccgagcatg atgttctcgg tcaccgagaa gaccggcacc agcatgaagt gctggtggac
     3781 catgccgaga ccggcggcga tggcatcgcc cggtgagtcg aagcggacgg gatcgccgtc
     3841 gatcaggatc tcaccctcgt cggcgtggta caggccatag aggatgttca tcagggtggt
     3901 cttgccggcc ccgttctcgc ccagcagggc atggatctcg ccgggctcca cggccaggct
     3961 gatccggtcg ttggcgacca cgccggggaa ccgcttggag atccctttta gttcaaggcg
     4021 catgctcccc gccccccggc ccgccgggac cgagactatc gcctagggga aggggggtgc
     4081 cgttggtgcc ggcacccccc ttcgaaccca ccggggtcgg tcgtcaggac aggtagtcct
     4141 tcggatcgac cgagatgctg ccgtcgatga tcccctgctt gagctcgtcg atcttgctct
     4201 tcaggtccga ggggacctgg ctgtcgaagt tgtggaacgg cgcgatgccc acgccgtcgt
     4261 tctcgagcgt tcccacgtac gtcccgccct ggaacgagcc gtcgatcacg gtcttgaccg
     4321 cgtcctcgac cgcgacgtcc atgttcttct ggacgctggt gagctcgatg tcctggtact
     4381 cgggagccga gacgaaccag tccgtgtcca cgccgatcag catggcgttg cccgaggcct
     4441 gcaccgccgc ggcggccccc aggccgaccg gtccggccac cggcatgatg atgtcggcgc
     4501 cctcggacag caggctctcg gtcacctgcc ggcccttgtt ctggtcctcg aagtcgccgg
     4561 tgaaggtgcc gtcctgcttg gcggggttcc agccgagcac ctggacgttc gtcccgttgt
     4621 cctggttgta cttcaacacg ccggctgcgt agccgttcat gaagatcgac acggtcggga
     4681 tgttgatgcc cccgaacgtg cccaccttgc cggtcttggt catccccgcg gcgaggtagc
     4741 cggccaggaa cgccgcctgg tccgtggaga aggtgagggc caggacgttc gggatcgtct
     4801 tcgggtacgc gtagtcgacg atcgcgaagt tctggtccgg gttcgccttg gccgcggcct
     4861 ccgtggcgtc gcccagcagg aacccgacgg tcacgatcag gtcgcacttg cccaggaagg
     4921 cctggatgtt cggcttgtag tcgtcctggg tggtggactc caagtacttg ccggcaacgc
     4981 cgtagtcctt ctcggcgtcg gtgacgccct tccaggccgt ggcgttgaag ctcttgtcgt
     5041 tgatcccgcc ggtgtcggtc acctcacagg cggtcacccc cgcgccgggt tgctccgacg
     5101 cggaggtgct gccgccgttc ccgccgctct cgttgttgct ggcgcaggcg gcggccagca
     5161 tcgcgaggac ggcgagcacg gcgacggatc ggaagatgcg gttcatggac ctgccctcct
     5221 ctcttgcccc tcgctgcatc ttacgtagcg gaggtggccg cagggtgggg gtcttccggc
     5281 ccgtccaccg cgggaccggg cccgcgaccg gagctgtgga ccggacccct cgagccgggc
     5341 cacgcacgct gttggggcct ccgggccgtg cggtccctca ttcggtgccg aacaggcgat
     5401 ccccgaagtc ccccaggccc ggcaggatgt agccgcgctc gttcagctgg cggtcgacgg
     5461 ccgcggtgaa gatcggcacg tcggggtgct cctgctgcat cttggccagg ccttcggggg
     5521 cggagaccac gcacacgaac cgcatcgagg ccgggtcacc ctcgcggacc gagtggcagg
     5581 cggccgagcc
//
LOCUS       DASQQQ010000209        14099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_345000, whole genome shotgun sequence.
ACCESSION   DASQQQ010000209 DASQQQ010000000
VERSION     DASQQQ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14099
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345000"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /gene="metK"
                     /locus_tag="VE646_07900"
     CDS             <1..1041
                     /gene="metK"
                     /locus_tag="VE646_07900"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HZD79948.1"
                     /translation="TGLVMVAGEITTSEYIDIPTVVRRTVLDIGYTDANFGIDGATCG
                     VVTSIQEQSVDIARGVDDAWEHRGGSGDDLDALGAGDQGMMFGYACRETDELMPIPIA
                     MAHRLTKRLADVRKAGVVPYLRPDGKSQVSVEYRGGRPVRVDTVLIAAQHREEVDVES
                     LLAPDVEAEVVDPVLREFDLEADGVRVLVNPTGRFEIGGPKADTGLTGRKIIVDTYGG
                     MAPHGGGCFSGKDPTKVDRSAAYMARYVAKNLVAAGVAERLQIQVAYAIGTAHPVSLA
                     VDTFGTETVDPQRIVETVWDVFDFRPAAIIRALDLRRPIYEETAAYGHFGRKEFSWEA
                     TDRADELRRALA"
     gene            1016..2890
                     /locus_tag="VE646_07905"
     CDS             1016..2890
                     /locus_tag="VE646_07905"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79949.1"
                     /translation="MSSAAPSPSRCPSSGPVAVCVDRPLLSLDRPFTYDLPEQLGAGL
                     GSLVQVPFHGRAARGWILGPTDDVPNRMLPVRKLVSPVRFFDQDRLRLLRWMAERYIA
                     PLATVIGRSYPPRVASEEDVVGVVGGGVGAPGSGFPSAAWRLRPGPLRTDPPARAFGP
                     LGRYRGGVQLIDALAEGSGTFVMRTAPGDEQGAAVEAVGAALGSGRTAIVVVPEAEPL
                     PATARAVVEAFGERAALFVGGSKRARYRTWLEILAGRYQVVVGTRPAVFAPVERLGLV
                     YVSRESHGGHREERSPAYHVRDVAVARARLEGGVSVLQALCPTAEAAQLGTTQVAPRG
                     RWWPPVEVVRPGPEGRSPRLVALLRKTRGAFLYAPVPGAGVARVCRTCGEPAACAVCG
                     GMLRAGAGRIRCVVCGADGRCAGCGSTTFGVQPGGAERVEDWARRIAPVPVRRIGPED
                     PPTPPPQGEVTVGGVEAVKDFGPRALDLVGVLDADRASRRPGLAAGERALAAWMEAAS
                     WVGPGGRVLVQTRTPNDPAVQALVMGDPERFRRSDLARRREAGFPPGWPVFRVVGTGT
                     LEAELRALVPATLLVSGLGDETVCLLALEPGALPGFARTVRTLAERGVVTRVEAEPHL
                     "
     gene            2913..3431
                     /gene="def"
                     /locus_tag="VE646_07910"
     CDS             2913..3431
                     /gene="def"
                     /locus_tag="VE646_07910"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014351715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZD79950.1"
                     /translation="MTILPIRILGDPVLREQARDLERFDEGLRRLVDDMFETMYAAPG
                     VGLAAPQVGVSSRFFIYDDGDGAKGFVANPVLSDPAGEETRDEGCLSIPGPFHPTTRA
                     LSISLRGQDLRGAPLQIRAEGLLARIFQHETDHTQGRLYIDRLDDDGRREVMRQLREA
                     ELRRKQEGSRRL"
     gene            3459..4397
                     /gene="fmt"
                     /locus_tag="VE646_07915"
     CDS             3459..4397
                     /gene="fmt"
                     /locus_tag="VE646_07915"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZD79951.1"
                     /translation="MAPRSLRVTFLGNDPWSVPSLEAVRGSGHRVGLVLTRGPRPGRR
                     GSGSIPTAVAQAATRLDLPLTEADTVRTGRGLEALTGSSPEVLVVVAYGEILPPSVLE
                     LPTVAAVNVHFSLLPELRGASPVQQALLRGFERTGVTTMLMEEGPDTGPILRQRAEPI
                     RDEDDAGRLGGRLARIGGDLLVASLEDLAEGTARPRPQDASRATFAPKLHPEDRRIDW
                     QVPAEDVVNRVRAFGPEPGALTAFRGAPLKVLRAAASPERGSPGVVVSAGEGGLVVAA
                     ARGAVALLQVAPAGKREMSGVAFVRGRRPQLGERLA"
     gene            4394..5686
                     /locus_tag="VE646_07920"
     CDS             4394..5686
                     /locus_tag="VE646_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZD79952.1"
                     /translation="MSTAPSAREVALRVVLRVVEHDGYSNLSLGSALAGSSLSAQDRA
                     LATDLAYGTLRRLPWLDRALGPLLVRPLDRATPRARTLLRIGAYQVLCTRIPPHAAVG
                     ETVALAGPRERGFVNAVLRRLASDPPGPPTGGSDQEVALRTGLAPWAVRELRRLVGPE
                     AEGAAGALGERAPLTIRTNTCRIGVDELARRLAEAGLEARPGTIHPGSLLLEGGNPSS
                     LPGFREGWFAVQDQASSFVVAALAPEPGHRVLDACAGPGGKTGHLACLVGPEGLTVGA
                     DVSPARAGLVRTQAHRLGVGVQVLAQDARRPALRGSFDRVLVDAPCSGLGSARRRPEL
                     LWRPRRQELSHLARLQVAITASAAELLAPGGRLVYSVCTFPRAETDAACDAILRHRPD
                     LEPEELSGPDGPGRRIRLWPHRHGTDAMFVAAFRRRST"
     gene            5716..6375
                     /gene="rpe"
                     /locus_tag="VE646_07925"
     CDS             5716..6375
                     /gene="rpe"
                     /locus_tag="VE646_07925"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HZD79953.1"
                     /translation="MGKLSVSILGCDLAELADQVKLVEPHADLIHVDVMDAHFVPIVT
                     FGSVVVASLRPRTGLVLDGHLMVDAPTVLFEDLAEAGMDRVTFHVEAVEDPDPVIRKA
                     RGVGMGVGMTMNPGTPVDALYPFLEELDNVIVMSVNPGWAGQAFIPEALPKLERMRRE
                     LDRRGLDPDLQIDGGVKKDNARRCVEAGANVLVVSSGIYAAQDIPAAAREFAAIARGA
                     A"
     gene            6375..6707
                     /locus_tag="VE646_07930"
     CDS             6375..6707
                     /locus_tag="VE646_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZD79954.1"
                     /translation="MPETILVVDDDPDIARFVEVNLRSAGYEVAVASDGEEALSRAME
                     LRPDLVLLDVMMPKVDGFEVAQRLRRNPQTANTSIIMLTAKALSTDKVLGLTAGADDY
                     IIKPFDPI"
     gene            6750..7307
                     /locus_tag="VE646_07935"
     CDS             6750..7307
                     /locus_tag="VE646_07935"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZD79955.1"
                     /translation="MRNLSPLTGLPGNIRIQEEIERMVREGRPFAVLYSDLDNFKAYN
                     DQKGFVRGDRLIQTTARIIQDAVVEFAGSEGFVGHVGGDDFVAIVPPGVAEGTAKRIV
                     DRFDAVVHGFYEEEDLARGYVEVEDRKGVLQKLPLVGVSVGVATTARRGFAHYGEAVS
                     VATEMKQFAKRDTGSSYAVDRRTAG"
     regulatory      7350..7478
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7548..8645
                     /gene="ribD"
                     /locus_tag="VE646_07940"
     CDS             7548..8645
                     /gene="ribD"
                     /locus_tag="VE646_07940"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HZD79956.1"
                     /translation="MDDRAYVERALGLAERGRGMTSPNPMVGAVVVADGRIVGEGHHE
                     GPGLPHAEVLALRDAGERARGATLYTTLEPCDHFGRTPPCTRAIVEAGVARVVSAMGD
                     PNPAVNGRGFAVLGDAGIEVRSGVLADVAERQNQAYLKHVSTGLPFVTWKMAASLDGK
                     VAARDGTSRWITGQAARRDVHRLRAWSDAILVGAGTALMDDPSLTVRDPEYRGRPPLR
                     VLLDARGRVPETGDLFDDAAPTLVATTEQAPRERRDAWRARGAEVVEYEPEAGLVPLG
                     TLLRDLGKRDVQGVLLEGGPTLAWAAVEEGAVDKVVVYLSPRLIGGRDAPSVLGGRGF
                     APVGAALGLRIWSFDRVGEDLRVEADVQRDR"
     gene            8626..9246
                     /locus_tag="VE646_07945"
     CDS             8626..9246
                     /locus_tag="VE646_07945"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HZD79957.1"
                     /translation="MFSGIVEERGAVGVVEPHRLVVRCRTVLEDSDLGASVAVNGVCL
                     TVVERTADTLTFDVSEETRSRTSLARLSPNDPVNLERPVTLMTRLGGHLVQGHVDGVG
                     RVVSLERDELGGALVRVRPPAELLRYLVEKGSVAVDGVSLTVAGPPDEAFSVALIPHT
                     LAVTTLGTAAVGDPVNLEVDVMAKYVERLLKATDQGRGTTEGMVDR"
     gene            9324..10538
                     /locus_tag="VE646_07950"
     CDS             9324..10538
                     /locus_tag="VE646_07950"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HZD79958.1"
                     /translation="MVIVVDDADRENEGDFVMAAEKVTPEAVNFMVTNGRGIVCMPVT
                     AERLDHLGIPLMVTRNNESHGTAFAVSIDVKDRTTTGTSAYDRAATVRAITDPDLTHD
                     DIRMPGHVFPLMAQEGGVLRRAGHTEASVDLARLAGLFPAGVLCEVLHPDGTMARMPE
                     LVRVAREHALSLISIADLIAYRRRTERLVRRVAEARIPTAQGEFRAVAYESAVDGMTH
                     LALVAGEIGEGENILVRVHSECLTGDVFGSLRCDCGSQLQAALASVAKEGRGVVLYMR
                     GHEGRAIGLTHKLRAYELQEEQGLDTVEANQELGFPADPRDYGIGAQILADLGVRSMR
                     LLTNNPSKRAGLEGHGLSIVERVPILTEPTPHNIDYLRTKRTKLGHILDHLGPEDPME
                     RALHLPAATEGP"
     gene            10538..11017
                     /gene="ribH"
                     /locus_tag="VE646_07955"
     CDS             10538..11017
                     /gene="ribH"
                     /locus_tag="VE646_07955"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HZD79959.1"
                     /translation="MPEVRGEAEAQGRRLAVVASRFNGIVVAKLLDGALEGLRSRGAG
                     EPDIDVVWVPGAFELPLVARRMAGSGLYDAVVCLGAVIRGETPHFDHVAQQAAEGIRR
                     AGEATGVPVIFGVLTTDTFEQAMDRAGGTHGNKGWDAAMAAMEMASLLDRLPKEGSP"
     gene            11014..11364
                     /locus_tag="VE646_07960"
     CDS             11014..11364
                     /locus_tag="VE646_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZD79960.1"
                     /translation="MIIDKPWGKVVTYALNQPASVRIITVESGQETSVHYHQMRDEMW
                     VVLDPGLSLQIGERLVDPDPGEEFMVAAEITHRITNHGSSRGRVLEIAYGYTTEDDTQ
                     RLEDDYGRPLEPDW"
     gene            11458..11664
                     /locus_tag="VE646_07965"
     CDS             11458..11664
                     /locus_tag="VE646_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZD79961.1"
                     /translation="MATQGTVKWFSNEKGYGFISRPDGEDIFVHFSAIQMDGFKTLTE
                     GQEVEFDVVDGPKGKQAANVHAAS"
     gene            11809..12351
                     /gene="infC"
                     /locus_tag="VE646_07970"
     CDS             11809..12351
                     /gene="infC"
                     /locus_tag="VE646_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HZD79962.1"
                     /translation="MNDRIRAPQVRLVGPDGAQIGIVPTQAALRQARDLDLDLVEVAP
                     QANPPVCRIMDYGKYKYERDIRQKEARKKQARVEVKEIKFRPKIDPHDYATKKGHVER
                     FLRAGARVKVTIMFRGREMAHTELGRKILDRLVADLEGRAVVESTPKQDGRNMVMVIA
                     PTKKQPAGKPEEPAVTREQG"
     gene            12356..12547
                     /gene="rpmI"
                     /locus_tag="VE646_07975"
     CDS             12356..12547
                     /gene="rpmI"
                     /locus_tag="VE646_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HZD79963.1"
                     /translation="MPKMKTHRGAAKRFRVTRTGKVLHRKATGNHFLMKKSGSQRRRI
                     EGMTEVTAERRTVRRLLGE"
     gene            12551..12910
                     /gene="rplT"
                     /locus_tag="VE646_07980"
     CDS             12551..12910
                     /gene="rplT"
                     /locus_tag="VE646_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004578709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HZD79964.1"
                     /translation="MARVKRSVHAKKKRREILSQAKGYYGSRSRRLRVAKEQVMHSGR
                     YAYRDRRDRKGQFRRLWIARIGAGARQNGMSYSQLVHGLDKAGVELDRKILADLAVND
                     PNAFAGLVQTARDALAV"
     gene            12966..>14099
                     /locus_tag="VE646_07985"
     CDS             12966..>14099
                     /locus_tag="VE646_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HZD79965.1"
                     /translation="MITSLKNPKVAAAIKLKKRAFRDRERRFLVEGAQAIGEALSSAG
                     GISQLVRSSDLHPVVTRAREAGVEVVEVSEAVMAKLTSTVTPQGLVAVAPYLDVPPDA
                     VPERPGCVAVLHAVRDPGNAGTVLRSADAAGADAVIFSDSSVDPYNPKTVRASAGSLF
                     HLPVVRGAPTPAAVAALRGRGCRVLAMDARGQADLYGMDLSGPVAFLFGNEAWGLPGE
                     VAALADGMVRVPIARRAESLNLAAAASVCLFEWARQRRQGRRSALETVIASAAHDIRS
                     PLTAMKGFGFALSSRWEQMSAEHRSLMLQGIVHDTDRMNIIVRQLVDAARIVAGNLEL
                     FPQLVKLEELVARVAEERRGDPGHPAVRWTGGRVRAFVDPDRLQ"
BASE COUNT         1916 a         4761 c         5437 g         1985 t
ORIGIN      
        1 acgggtctgg tcatggtggc gggcgagatc accacgtccg agtacatcga catccccacg
       61 gtggtgcggc gcaccgtgct ggacatcgga tacacggacg cgaacttcgg catcgacggt
      121 gccacctgcg gcgtcgtgac ctccatccag gagcagtcgg tcgacatcgc ccgcggcgtg
      181 gacgatgcct gggagcaccg gggcggctcg ggggacgacc tcgatgccct cggcgccggc
      241 gaccagggca tgatgttcgg ctacgcctgc cgcgagaccg acgagctcat gcccatcccc
      301 atcgccatgg cgcaccggct gaccaagcgt ctggccgacg tccgcaaggc gggcgtggtc
      361 ccgtacctgc gacccgacgg caagagccag gtcagcgtcg agtaccgggg cgggaggccg
      421 gtgagggtgg acaccgtgct gatcgcggcc cagcaccgcg aggaggtcga cgtggagtcg
      481 ctgctcgcgc ccgacgtcga ggccgaggtg gtggatcccg tcctgcgcga gttcgacctg
      541 gaggccgacg gcgtccgggt cctggtgaac cccaccggcc ggttcgagat cggcgggccg
      601 aaggcggaca ccgggctcac cgggcggaag atcatcgtcg acacgtacgg aggcatggct
      661 ccccacggcg gcggctgctt ctcgggcaag gacccaacga aggtggaccg gtcggccgcc
      721 tacatggccc ggtacgtggc gaagaacctg gtggcggctg gggtggccga gcgcctccag
      781 atccaggtgg cctacgcgat cgggaccgcg caccccgtgt ccctggcggt ggatacgttc
      841 ggcaccgaga ccgtggatcc ccagcgcatc gtggagacgg tgtgggacgt cttcgacttc
      901 cggcccgccg cgatcatccg tgccctggac ctgcggcggc ccatctacga ggagacggcc
      961 gcctatggcc acttcggccg caaggagttc tcttgggagg ccacggaccg cgcggatgag
     1021 ctccgccgcg ccctcgccta gccgctgccc atcctccggc ccggtcgccg tctgcgtcga
     1081 ccgtccgttg ctctcgctcg atcgtccctt cacctacgac ctgcccgagc agctcggcgc
     1141 cggcctcggc tccctggtgc aggtcccgtt ccacggccgc gcggcccgag gctggatcct
     1201 cggacccacc gacgacgtcc cgaaccgcat gctcccggta cgcaagctcg tgtctccggt
     1261 ccgcttcttc gaccaggacc ggctccggct cctgcgctgg atggccgagc gctacatcgc
     1321 cccgctcgcc accgtcatcg gccgctcgta tccgcctcgg gtggcgtccg aggaggacgt
     1381 ggtgggggtg gtgggcgggg gggtcggcgc tccgggatcc gggttcccct ccgccgcttg
     1441 gcggctccga cccggaccgc tccgcaccga cccccccgcc cgcgcgttcg gtccgctcgg
     1501 ccggtatcgg ggcggggtcc agctgatcga tgcgctggcg gagggatcgg ggacgttcgt
     1561 catgcggacg gcgcctgggg acgagcaggg ggcggccgtg gaggcggtcg gggccgcgct
     1621 gggttcgggg cggacggcca tcgtggtggt gccggaggcg gagccgctgc cggccaccgc
     1681 gagggcggtg gtggaggcgt tcggggaacg ggccgcgctg ttcgtggggg gatccaagcg
     1741 cgcgcggtac cggacctggc tggagatcct ggcggggcgg taccaggtgg tggtggggac
     1801 gaggccggcg gtgttcgcgc cggtggagcg gttggggctc gtgtacgtgt cacgggagag
     1861 ccacgggggg caccgggagg agcgctcgcc cgcgtaccac gtgcgggacg tggcggtggc
     1921 gagggcgcgg ctcgagggag gcgtcagtgt gctgcaggcc ctgtgtccga cggcggaggc
     1981 cgcccagctg ggaacgaccc aggtggcgcc tcggggacgg tggtggccgc cggtggaggt
     2041 ggtgcggccg ggtcccgagg ggaggtcccc gcgcctggtg gcgctcctgc ggaagacgcg
     2101 gggcgcgttc ctgtacgccc cggtgccggg ggccggggtg gccagggtct gccggacctg
     2161 tggcgagccc gcggcctgcg ccgtgtgcgg cgggatgctc cgtgcggggg cgggtcggat
     2221 ccgctgcgtg gtctgcggcg cggatggccg gtgcgccggg tgcgggagca ccaccttcgg
     2281 cgtgcaaccc ggaggcgccg agcgggtgga ggactgggcc cggcgcatcg ccccggtgcc
     2341 ggtccggcgg atcggcccgg aggatccacc gacgccgccc ccgcagggag aggtcaccgt
     2401 gggcggggtg gaggcggtca aggacttcgg tccgcgagcg ctggacctgg tgggcgtcct
     2461 ggacgcggac cgggcgtccc ggcggccggg gttggccgcg ggggagcgcg ccctggcggc
     2521 gtggatggag gcggcgtcct gggtcggccc cggcgggcgg gtgctggtcc agacccgaac
     2581 ccccaacgat ccggccgtcc aggccctcgt gatgggcgat cccgagcgct tccgtcgttc
     2641 cgacctcgcg cggcgcagag aggcggggtt cccgcccggg tggccggtct tccgggtggt
     2701 ggggacgggc acgctcgagg ccgagcttcg agcgctggtg cccgccacgc tcctggtctc
     2761 cgggctcggg gacgagacgg tatgcttgct cgcgctcgag cccggcgccc taccgggctt
     2821 cgctcgcacc gttcggaccc tcgccgagcg cggcgtggtg actcgcgtgg aggcggagcc
     2881 ccacctgtga ggagcatggc gaggaacgac cgatgacgat cctgcccatc cgcatcctcg
     2941 gcgatccggt gctccgggag caggcccggg acctcgaacg gttcgacgag gggctccgcc
     3001 gcctggtcga tgacatgttc gagacgatgt acgcggctcc cggcgtcggg ctggccgctc
     3061 cgcaggtggg ggtctcctcg cgcttcttca tctacgacga cggcgatggg gccaagggct
     3121 tcgtggccaa cccggtgttg tccgatccgg cgggcgagga gacccgggac gagggatgtc
     3181 tgtcgatccc cggccccttc cacccgacca ccagggcgct ctcgatctcg ctgcgggggc
     3241 aggacctgcg aggagcgccc ctccagatcc gcgccgaggg gctcttggcg cggatcttcc
     3301 agcacgagac cgaccacacg cagggccgcc tgtacatcga tcggctggac gatgatggcc
     3361 ggcgtgaggt gatgcgccag ctccgcgagg ccgagctccg ccgcaagcag gaggggtccc
     3421 ggcgcctgta ggcggaggcg gaccgtatcc tgcgttccgt ggcacctcgg tcgctgcgtg
     3481 tgacgttcct gggcaacgat ccctggtcgg ttcccagcct ggaggccgtg aggggttccg
     3541 ggcaccgcgt gggcctggtc ctgacccggg gcccgaggcc cggccgccgc gggagcgggt
     3601 cgatccccac ggccgtggcc caggcggcca cgcgcctgga cctgccgctg acggaggcgg
     3661 acacggtccg aaccgggcgt ggtctggagg ccctgacggg ctcgtccccc gaggtcttgg
     3721 tcgtggtggc ctacggggag atcctgcccc cgagcgtgct cgagctgccc accgtggccg
     3781 ccgtgaacgt gcacttctcg ctcctaccgg agctgcgggg cgcgagcccc gtgcagcagg
     3841 ccctgctccg gggcttcgag cgcaccggcg tcaccacgat gctgatggag gaggggccgg
     3901 acaccggccc catcctccgg cagcgggccg agccgatccg cgacgaggac gacgccggac
     3961 ggctgggggg gcgcctggcc cggatcggcg gcgacctgct ggtggcctcc ttggaggatc
     4021 tggccgaggg cacggcacgg ccgaggcccc aggacgcgtc gagggcgacg ttcgccccca
     4081 agctgcaccc cgaggaccga cggatcgact ggcaggtccc ggcggaggac gtcgtgaacc
     4141 gggtgcgggc gttcgggccc gagcccggcg ccctgaccgc gttccggggc gcccccctga
     4201 aggtgctccg ggcggccgcg agccccgagc ggggctcgcc gggcgtcgtg gtctcggcgg
     4261 gggagggcgg gctcgtggtg gccgccgccc gaggagcggt ggccctgctc caggtggccc
     4321 ccgccgggaa gcgggagatg agcggggtgg ccttcgtgcg gggccgccga ccccagctcg
     4381 gcgagcggct ggcgtgagca ccgcgccctc ggcgcgagag gtggccctcc gggtggtgct
     4441 gcgggtggtc gagcacgacg gctactccaa cctgagcctc ggatcggctc tggccgggtc
     4501 gagcctgtcg gcgcaggacc gagcccttgc caccgacctg gcctacggca ccctacgacg
     4561 gctgccgtgg ctcgatcggg ctctgggccc gctcctggtc cgtccgttgg atcgggccac
     4621 gccccgggct cgaacgctgc tccggatcgg cgcctaccag gtcctctgca cgcggatccc
     4681 gccccacgcc gcggtcgggg agaccgtggc gctggccggc ccccgcgagc ggggcttcgt
     4741 gaacgcggtg ctgcggcggc tggcgtccga tcccccgggc ccgccgaccg gcggctcgga
     4801 ccaagaggtc gccctgcgga ccgggctggc cccgtgggcc gtccgcgagc tccgccgcct
     4861 ggtgggtccc gaggccgagg gcgcggccgg cgccctcggc gagcgggcgc cgctcacgat
     4921 ccggaccaac acctgccgga tcggggtcga cgagctcgcg cgtcggctgg ctgaggcggg
     4981 gttggaggca cgtccaggca ccatccaccc cggatccctc ctgctcgagg gagggaaccc
     5041 gtcgagcctc ccagggttcc gggaggggtg gttcgcggtg caggaccagg cctcgtcctt
     5101 cgtggtcgcc gccctggctc ccgagcccgg tcaccgtgtg ctcgatgcct gtgccggccc
     5161 gggagggaag accgggcacc tggcctgcct ggtgggcccc gagggactca cggtcggcgc
     5221 cgacgtctcg ccggcgcggg ccggcctggt gcggacccag gcccaccggc ttggcgtagg
     5281 ggtccaggtc ctggcgcaag acgctcgacg accggccctg cgcggctcgt tcgaccgggt
     5341 gctggtggac gcgccctgct ccggcctggg atcggcccgg cggcgccccg agctgctgtg
     5401 gcggccccgg cggcaggagc tgtcgcacct ggcccgactc caggtcgcga tcaccgcctc
     5461 ggctgccgag ctcctggccc ccggcggtcg cctggtctac tcggtctgca ccttcccccg
     5521 cgccgagacc gacgccgcgt gcgacgcgat cctgcgccac cgcccggacc tggagcccga
     5581 ggagctgtcc gggccggacg gtcccggtcg ccggatccgc ctgtggccgc accggcacgg
     5641 caccgacgcg atgttcgtgg ccgcgttccg ccggcgctcc acctgagccc gtacggccgc
     5701 ggatacaatc cgcccgtggg gaagctctcg gtctcgatcc tcggctgcga cctggccgag
     5761 ctggccgatc aggtcaagtt ggtcgagccc catgccgacc tcatccacgt cgacgtcatg
     5821 gacgcgcact tcgtccccat cgtcacgttc ggatcggtgg tggtcgcctc gctgcggccg
     5881 aggacgggac tggtcctgga cggccatctg atggtcgacg caccgacggt cctgttcgag
     5941 gacctggccg aggccggcat ggaccgggtg accttccacg tcgaggctgt ggaggacccc
     6001 gacccggtga tccgcaaggc caggggggtg ggcatgggcg tggggatgac gatgaacccc
     6061 gggaccccgg tggacgcgct ctacccattc ctggaggagc tcgacaacgt gattgtgatg
     6121 agcgtgaacc cggggtgggc cgggcaggcg ttcatccccg aggccctgcc caagctcgag
     6181 cggatgcggc gcgagctgga ccggcggggg ctcgaccccg acctgcagat cgacggtggg
     6241 gtgaagaagg acaacgcccg gcgctgcgtg gaggccgggg cgaacgtgct ggtggtgtcc
     6301 tcggggatct acgcggccca ggacatcccg gccgccgcac gcgagttcgc ggcgatcgcg
     6361 aggggggcgg cctgatgccc gagacgatcc tggtcgtgga cgatgatccc gacatcgctc
     6421 ggttcgtgga ggtgaacctg cgctcggccg gctacgaggt ggccgtggcc tcggacggcg
     6481 aggaggcgtt gtcccgagcc atggagctcc gcccggacct cgtgctgctg gacgtgatga
     6541 tgcccaaggt cgacgggttc gaggtggccc agcggctgcg tcggaaccca cagaccgcca
     6601 acacctcgat catcatgctg accgccaagg ccctgtccac ggacaaggtc ctggggctca
     6661 ccgcgggggc ggacgactac atcatcaagc cgttcgaccc catctagctg ctggcgcggg
     6721 tcaaggggac cctccgccgg gccaaggaga tgcggaacct gtcccccctc accggcctgc
     6781 ccggcaacat ccggatccag gaggagatcg agcgcatggt ccgcgagggc cggcccttcg
     6841 ccgtcctgta ctcggacctg gacaacttca aggcctacaa cgaccagaag gggttcgtgc
     6901 ggggcgaccg gctgatccag accacggccc gcatcatcca ggacgccgtg gtggagttcg
     6961 cgggatccga gggcttcgtg gggcatgtgg ggggggacga cttcgtcgct atcgtcccgc
     7021 ccggtgtggc cgagggcacc gccaagcgca tcgtggatcg tttcgacgcg gtggtgcacg
     7081 ggttctatga ggaggaggac ctggcccgcg ggtacgtgga ggtcgaggac cgcaagggag
     7141 tcctgcagaa gctgcccctg gtcggggtgt ccgtgggcgt ggccaccacg gcacggcggg
     7201 ggttcgccca ctacggggaa gccgtatcgg tggccaccga gatgaagcag ttcgccaagc
     7261 gtgacaccgg ctcctcctac gcggtggacc ggcgcaccgc cgggtaggcc tggcccaacc
     7321 gaccgccgcg tccctatact ggggcagaag ctcgttctcg gggcggggtg caaatcccca
     7381 ccggcggtga gagtccgcga ccccccggca gccggtcggg ggttgacccg gtgagatccc
     7441 gggaccgacc gtgagagtcg ggatgggaga ggacgggcgg gccggtggcg gtcgccgcgg
     7501 cctgcgccct cgttccttcc tgcgccgcgt ccccggtgag gtgtgacgtg gacgaccgtg
     7561 cgtacgtgga gcgggccctg gggctcgcgg agcgggggag gggcatgacc tctccgaacc
     7621 ccatggtcgg cgccgtggtg gtggccgacg gtcgcatcgt gggtgagggt caccacgagg
     7681 gcccggggct gcctcatgcc gaggtgctcg ccctgcgcga cgcgggggag cgcgctcggg
     7741 gggccacgct gtacaccacg ctcgagccgt gcgatcactt cggccgaacc ccaccgtgca
     7801 cgcgcgcgat cgtggaggcc ggggtcgccc gagtggtctc ggccatgggc gacccgaacc
     7861 cggccgtgaa cggacggggt ttcgccgtgc tcggggacgc cggcatcgag gttcgctcgg
     7921 gcgtgctggc cgacgtggcc gagcggcaga accaggcgta cctgaagcac gtctccaccg
     7981 ggctgccctt cgtgacgtgg aagatggccg cctccctgga cggcaaggtg gcggctcggg
     8041 acggcacctc ccgctggatc acgggccagg ccgcccgccg cgacgtgcac cggctgcggg
     8101 cctggtcgga cgccatcctc gtgggagccg gcaccgccct gatggacgac ccctccctga
     8161 cggtgcggga ccccgagtac cggggacgcc cacccctgcg ggtcctgctg gacgcccggg
     8221 gacgggtccc cgagaccggg gacctgttcg acgacgccgc gcccaccctg gtggccacca
     8281 ccgagcaggc cccgcgcgaa cgccgggacg cctggcgggc ccgcggggcc gaggtcgtcg
     8341 agtacgagcc cgaagccggc ctggtcccgc tcgggacgct cctgcgcgac ctgggcaagc
     8401 gcgacgtcca gggcgtgttg ctggaagggg gccccacgct ggcctgggcc gcggtggagg
     8461 agggggcggt ggacaaggtg gtcgtgtacc tgtcaccgag gctgatcggc ggtcgggacg
     8521 cgcccagcgt cctgggcggt cgggggttcg cgccggtggg cgcagcgctg gggctccgga
     8581 tctggtcgtt cgatcgggtg ggcgaggacc tacgggtgga ggccgatgtt cagcgggatc
     8641 gttgaggagc ggggggccgt cggcgtggtc gagcctcacc ggctggtggt gcggtgccgg
     8701 accgtgctgg aggactcgga cctgggggcc tcggtggcgg tgaacggggt gtgcttgacc
     8761 gtggtcgagc gcaccgccga caccctgacc ttcgacgtgt ccgaggagac ccggtcccgc
     8821 accagcctgg ctcggctctc gcccaacgat ccggtgaacc tggagcgtcc ggtcacgctg
     8881 atgacgcggt tggggggcca cctggtgcag gggcacgtgg acggggtggg ccgggtggtg
     8941 agcctcgagc gggacgagct cggcggggcc ctggtgcggg tgcgcccgcc ggccgagctg
     9001 ctgcgctacc tggtggagaa gggttcggtg gcggtggacg gggtgagcct caccgttgcc
     9061 ggcccgccgg atgaggcgtt ctcggtggcc ctgatcccgc acacgctggc ggtcaccacg
     9121 ctggggaccg ccgccgtggg cgacccggtg aacctcgagg tggatgtcat ggccaagtac
     9181 gtggagcggc tgctgaaggc gaccgaccag ggtcgcggaa cgacggaggg gatggtggat
     9241 cggtgagcga gacggagcag gtggtgggcc ggggggtgtt cgcctcgatc gaggaggcga
     9301 tcgaggacat ccgggcgggc cgcatggtga tcgtggtgga cgatgccgac cgggagaacg
     9361 agggcgactt cgtgatggcg gccgagaagg tgacgcccga ggcggtcaac ttcatggtca
     9421 cgaacggtcg gggcatcgtg tgcatgccgg tgacggcgga gcgcctggac cacctgggga
     9481 tcccgctcat ggtcacccgg aacaacgaga gccacggcac ggccttcgcc gtctcgatcg
     9541 acgtcaagga ccgcaccacc accggcacca gcgcctacga ccgggcggcc acggtgcgag
     9601 ccatcaccga tcccgacctc acccacgacg acatccggat gcccgggcac gtcttcccgc
     9661 tcatggcgca ggagggcggg gtgctccgcc gcgccgggca caccgaggcc tcggtggacc
     9721 tggctcggct ggccgggctg ttccccgccg gcgtcctgtg cgaggtgctg caccccgacg
     9781 gcacgatggc gcggatgccc gagctggtgc gggtggcccg tgagcacgct ctctcgctga
     9841 tctcgatcgc cgatctcatc gcgtaccgac gacggaccga gcggctggtg cggcgcgtgg
     9901 ccgaggccag gatccccacc gcccagggcg agttccgggc cgtggcctac gagagcgccg
     9961 tggacggcat gacccatctc gcgctggtgg ccggggagat cggggagggg gagaacatcc
    10021 tggtccgggt ccactccgag tgcctgaccg gggacgtgtt cgggtccctg cggtgcgatt
    10081 gcggatctca gctccaggcc gcgctggcct cggtggcgaa ggagggccgg ggcgtggtcc
    10141 tgtacatgcg cgggcacgag gggcgggcca tcgggctgac ccacaagctg cgagcctacg
    10201 agctgcagga ggagcagggg ctcgacacgg tggaagcgaa ccaggagctg gggttcccgg
    10261 ccgacccgcg cgactacggg atcggcgcgc agatcctggc ggatctgggg gtccgatcca
    10321 tgcggctgct caccaacaac ccgtcgaagc gagcgggtct ggagggtcac ggcctgtcca
    10381 tcgtggagcg ggtcccgatc ctcaccgagc cgacgcctca caacatcgac tacctgcgga
    10441 ccaagcggac gaagctcggg cacatcctgg atcacctggg cccggaggac cccatggagc
    10501 gggcgctgca cctgcccgcc gccaccgagg gaccctgatg cccgaggtgc ggggagaggc
    10561 cgaggcccag ggccgccgct tggcggtggt ggcttcccgc ttcaacggga tcgtcgtggc
    10621 caagctgctg gacggggcgc tggaggggtt gcgcagccgc ggggccggtg agccggacat
    10681 cgacgtggtc tgggtcccgg gagccttcga gctgcccctg gtcgcccggc ggatggcggg
    10741 gtccgggctc tacgacgcgg tggtctgcct gggagcggtg atccggggcg agacccccca
    10801 cttcgaccac gtggcccagc aggccgcgga gggcatccgc cgggccggcg aggccacggg
    10861 cgtgccggtg atcttcggcg tcctcaccac cgacacgttc gagcaggcca tggaccgggc
    10921 cgggggcacg cacggcaaca aggggtggga cgcggcgatg gcggccatgg agatggcctc
    10981 gctcctcgac cggttgccga aggagggatc gccatgatca tcgacaagcc ctggggcaag
    11041 gtcgtgacgt acgcgctgaa ccagcccgcg tccgtgcgga tcatcaccgt ggagtccggt
    11101 caggagacca gcgtgcacta ccaccagatg cgcgacgaga tgtgggtggt cctggacccc
    11161 ggtctcagcc tgcagatcgg cgagcgcctg gtggacccag atccggggga ggagttcatg
    11221 gtggccgcgg agatcaccca ccggatcacg aaccacggat cgtcccgggg gcgcgtgctg
    11281 gagatcgcct acgggtacac cacggaggac gacacccagc ggttggagga tgattacgga
    11341 cgacccctgg agcccgactg gtgagcccga tcgaggccag gcgcgagcgt cgttcccccg
    11401 ttgtgccgca gcatcacgca tgctacgctc gcgcggcttt gaccccagga ggtcggattg
    11461 gcaacgcagg gcaccgtcaa gtggttcagc aacgagaagg gctacgggtt catctcccgg
    11521 ccggacggcg aggacatctt cgtccacttc tcggccatcc agatggatgg gttcaagacc
    11581 ctgaccgagg gacaggaggt cgagttcgac gtcgtggacg ggccgaaggg caagcaagcc
    11641 gcgaacgtcc acgccgcctc ctgagatcgg ccgggccgcc ggccgtcgac accgtccgtg
    11701 ggcgctccta tacttcccga gaacggttca gcgtgctcac atcggcaccg gccccggtcg
    11761 gtgccttctt gtcgcaggag gtgatgcggg gtctcggctg atccgcgcat aaacgaccgg
    11821 atccgggcac cgcaggtgcg tctggtcggg cccgacggcg cccagatcgg catcgttccg
    11881 acccaggccg ccctccggca ggcccgcgat ctggacctgg acctggtgga ggtggctccg
    11941 caggccaacc cgcccgtgtg ccggatcatg gattacggaa agtacaagta cgagcgggac
    12001 atccggcaga aggaggcccg caagaagcag gcccgggtcg aggtcaagga gatcaagttc
    12061 cgtcccaaga tcgacccgca cgactatgcc acgaagaagg ggcacgtgga gcggttcctg
    12121 cgggccgggg cgcgggtcaa ggtcaccatc atgttccggg ggcgcgagat ggcgcacacg
    12181 gagctcggtc ggaagatcct ggatcgcttg gtggcggacc tggagggcag ggccgtggtc
    12241 gagtccacgc ccaagcagga cggacgcaac atggtgatgg tgatcgcccc gacgaagaag
    12301 cagcccgcgg gcaagcccga ggagccggcg gtcaccaggg agcagggatg acgacatgcc
    12361 gaagatgaag acgcaccgcg gagccgcgaa gcggttccgt gtgacccgga ccggcaaggt
    12421 gctgcaccgg aaggccaccg ggaaccattt cctgatgaag aagagcggca gccagcgacg
    12481 gcgcatcgag gggatgaccg aggtcaccgc cgagcgccgc acggtccggc gcctgctcgg
    12541 ggagtagatc atggccaggg tcaagcgatc ggtccacgcg aagaagaagc gccgggagat
    12601 cctctcccag gccaagggct actacggctc tcgctcccgc cggctccgcg tggccaagga
    12661 acaggtcatg cactccgggc ggtacgcgta ccgggaccgc cgagaccgca agggtcagtt
    12721 ccggcgcctg tggatcgctc ggatcggcgc cggcgccagg cagaacggca tgtcgtactc
    12781 ccagctggtc cacgggttgg acaaggccgg tgtcgagctg gaccggaaga tcctggcgga
    12841 cctggcggtc aacgatccca acgcgttcgc cggcctggtc cagaccgcga gggacgcgct
    12901 cgccgtctga gtcctcggcc gttctcgggc ggggaggtcg cgagggtcgc ctcggagggg
    12961 gcatcgtgat cacgtcgctg aagaacccga aggtggcggc ggcgatcaag ctcaagaagc
    13021 gagccttccg cgaccgcgag cgccggttcc tggtcgaggg tgcccaggcg atcggcgagg
    13081 cgctgtcctc ggccggcggg atctctcagc tcgtgcgctc ctcggacctc cacccggtcg
    13141 taacccgcgc ccgggaggcg ggggtggagg tcgtggaggt ctcggaagcg gtgatggcca
    13201 agctcacctc gaccgtgacc ccccaggggc tggtggcggt ggcaccgtac ctggacgtgc
    13261 cgccggatgc ggtgccggag cggcccggct gcgtggccgt ccttcatgcg gtccgcgatc
    13321 ccgggaacgc ggggacggtg ctccgctccg cggacgcggc cggcgccgac gcggtgatct
    13381 tctccgactc ctcggtggat ccgtacaacc ccaagaccgt ccgcgcctcg gccgggtcgc
    13441 tgttccatct gcccgtggtc cgcggcgctc ccaccccggc ggccgtggcc gcgttgcggg
    13501 gccgcgggtg cagggtgctg gcgatggacg cccgggggca ggcggacctg tacgggatgg
    13561 acctgagcgg gccggtcgcc ttcctcttcg gcaacgaggc ctggggtctg ccgggggagg
    13621 tcgccgcgct ggccgatggg atggtgcggg tgccgatcgc ccgccgcgcc gagtcgctga
    13681 acctggctgc cgccgcctcc gtgtgcctgt tcgagtgggc ccggcagcgc cggcagggtc
    13741 gtcgctcggc cctggagacc gtgatcgcct ccgccgccca cgacatccgg tcaccgctga
    13801 ccgccatgaa gggtttcggc ttcgcgctgt ccagccggtg ggagcagatg agcgccgagc
    13861 accgttcgct gatgttgcag ggcatcgtcc acgacaccga ccgcatgaac atcatcgtcc
    13921 gccagctcgt ggacgccgcc cggatcgtgg ccgggaacct cgagctcttc ccccagctgg
    13981 tcaagctcga agagctcgtg gcccgggtgg ccgaggagcg gcgcggcgat cccgggcatc
    14041 ccgcggtgcg atggaccggg ggccgggtgc gcgcgttcgt cgatcccgac cggctccag
//
LOCUS       DASQQQ010000210         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_211401, whole genome shotgun sequence.
ACCESSION   DASQQQ010000210 DASQQQ010000000
VERSION     DASQQQ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211401"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VE646_07990"
     CDS             <1..412
                     /locus_tag="VE646_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014443232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZD79966.1"
                     /translation="FVISEYGAAIWCDPNVWIPEARRLLRHGGSLVFLGSAPLAMVCT
                     PLDGSVPSTERLERPYFTLHRLDWRDAIDDPGGIEFNLPISGWFRLFRETGFDVVDFV
                     ELQAPVPGPEVNFFVTADWAHRFPSEQVWKLRKR"
     gene            complement(475..1347)
                     /locus_tag="VE646_07995"
     CDS             complement(475..1347)
                     /locus_tag="VE646_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007226478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZD79967.1"
                     /translation="MTNEATDQTAVDAPKPIVYGVASALSPMVRRIVAHNPGLMTGPG
                     TNTYLVGIDEIAVIDPGPGDAEHLDAIAGCGGDRIRWILLTHTHEDHSPGAVGLKKRT
                     GAEILAFDGGEGKGKVRIDGQLGDESIIEATEFHLTALHTPGHASNHLCYLLNEERTL
                     FTGDHIMQGSTVVIRPPDGDMAAYLASLERLKHIRPRLRAIAPGHGHVITDPMGVVDE
                     YIEHRLAREQQIVDTLAARGTATVTELVEDIYVDVPTELHRWARFSVWAHLRKLADEG
                     RAKGDDLDGSWSPA"
     gene            complement(1344..1841)
                     /locus_tag="VE646_08000"
     CDS             complement(1344..1841)
                     /locus_tag="VE646_08000"
                     /inference="COORDINATES: protein motif:HMM:NF016102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HZD79968.1"
                     /translation="MALLLVLLFLVAPIAELAVIVQVAGAIGVLDTIGLLIAVSIVGG
                     WLAKHQGLTTLRRIQTAIAGGEMPSREVANGALILLAGALMIAPGFISDFVALLLLLP
                     PTRAAVRRPLMRWLAERGRGRVTVFGPRGPRTRGSATDDVWDVESWEEPPTSAPARGE
                     LRGPA"
BASE COUNT          291 a          711 c          763 g          326 t
ORIGIN      
        1 cttcgtgatc tccgagtacg gggcggcgat ctggtgcgat cccaacgtct ggatccccga
       61 ggcacgccgc ctgctgcgcc acggcggttc cctcgtgttc ctcgggagcg cgccgctggc
      121 gatggtgtgc acgcccctgg acggatcggt gccgagcacc gagcggctcg agcgccccta
      181 cttcaccctg caccgcctcg actggcgtga cgccatcgac gacccgggcg ggatcgagtt
      241 caacctgccg atctcgggct ggttccggct gtttcgcgag accggcttcg acgtcgtcga
      301 cttcgtcgag ctccaggctc ccgttccggg gcccgaggtc aacttcttcg tgaccgccga
      361 ctgggcacac cgcttcccgt cggagcaggt ctggaagctc cggaagcgct agagatctcg
      421 acgggatcgc cggacgggcc cgaccgtccg gcccactcgg ccgacgccag gccgtcaggc
      481 cggtgaccag ctgccgtcga ggtcgtcgcc cttggcgcgt ccctcgtcgg ccagcttccg
      541 caggtgcgcc cacacagaga accgggccca acggtgcagc tcggtgggaa cgtcgacgta
      601 gatgtcctcg accagctcgg tgacggtggc cgtcccccgg gccgccaggg tatccacgat
      661 ctgttgctcc cgggccagcc ggtgctcgat gtactcgtcc acaacgccca tcgggtcggt
      721 gatgacgtgg ccgtgcccgg gggcgatggc tcgcaggcgc ggccggatgt gcttgagccg
      781 ttcgagcgag gccaggtagg ccgccatgtc gccgtcgggc ggccggatga ccaccgtgga
      841 gccctgcatg atgtggtcgc ccgtgaagag ggtgcgctcc tcgttgagca gatagcagag
      901 gtggttggag gcatggcccg gggtgtggag ggccgtgagg tggaactcgg tcgcctcgat
      961 gatcgattcg tcaccgagct ggccgtcgat ccgcaccttg cccttgccct cacccccgtc
     1021 gaacgccagg atctcggcgc ccgtccgctt cttgagcccg acggcgccgg gcgagtggtc
     1081 ctcgtgggtg tgggtgagca ggatccagcg gatgcggtcg ccgccgcagc cggcgatggc
     1141 gtcgaggtgc tcggcgtccc ccggaccggg atcgatcacc gcgatctcgt cgatgcccac
     1201 caggtaggtg ttggttcccg gcccggtcat cagcccgggg ttgtgggcga cgatgcggcg
     1261 caccatcggc gagagggcgc tggccacgcc gtagacgatc ggcttggggg cgtcgaccgc
     1321 cgtctggtca gtggcctcgt tggtcacgcc gggcccctca gctcccctct ggcgggcgct
     1381 gacgtcgggg gctcctccca gctctcgacg tcccagacgt cgtcggtggc cgacccccgt
     1441 gtgcgggggc cacggggccc gaacacggtg acgcggccgc gcccacgctc ggccagccat
     1501 cgcatgagcg gccggcggac ggccgcccgc gtgggtggca gcaggagcag cagggccacg
     1561 aagtcgctga tgaagccggg agcgatcatc agggcgccgg cgagcaggat cagcgcgccg
     1621 ttggccacct cgcggctcgg catctcgccg ccggcgatgg cggtctggat gcgccgcagc
     1681 gtagtgaggc cctggtgctt ggccagccac ccaccgacga tcgagaccgc gatgagcaac
     1741 ccgatggtgt cgagcacccc gatcgcgccg gccacctgga cgatcacggc cagctcagcg
     1801 atgggggcga ccaggaagag cagtacgagc aggagagcca tgtgccgttg gatcggtgcg
     1861 ggtgaggatc ggcgatgtgt cgggcgggcg gacacctcga acggtggcga ccggacgtcc
     1921 actcagccta ccgccaggtc atcccagccc gatgggcgcc cctggtgccg ccgggctcgg
     1981 gggtgtgacc cccttcacac gccggctacc agggagtaac atgtccggaa ggtacggggg
     2041 gctctactcg taccggggtg gttgcagcgt cgcaggcgaa gggggagagc a
//
LOCUS       DASQQQ010000211         4238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_399637, whole genome shotgun sequence.
ACCESSION   DASQQQ010000211 DASQQQ010000000
VERSION     DASQQQ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399637"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..928)
                     /locus_tag="VE646_08005"
     CDS             complement(185..928)
                     /locus_tag="VE646_08005"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016434517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZD79969.1"
                     /translation="MAEIHDRIREWWDADAAVYDHSPSHAASDPLEAAAWRAALSAAL
                     PARGARVLDVGAGTGSMTMLAAELGYEVTALDLSPLMLERAQHKAAERAVRATFVVGP
                     AEEPPPGPFDAVMERHMLWTLPDPDRALRAWRGSVAPGGRLVLFEGIWGKDDLPERVK
                     RRAAEVLVRLYGIPHDHHAEYDPEILSQLPLGRSPSAEPLIHAVYRAGWRGSRIRRLR
                     DVEWARRIASRPLLGWLEQVPQYVLVADA"
     gene            complement(944..1759)
                     /gene="trxA"
                     /locus_tag="VE646_08010"
     CDS             complement(944..1759)
                     /gene="trxA"
                     /locus_tag="VE646_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZD79970.1"
                     /translation="MEGDVEMDMMQGTQTVVDVTDADFMQTVVEGSKQRPVVVDLWAS
                     WCGPCKTLGPMLERVAEQRGGQFLLAKLDVDANPYTASQFGVQSIPTVIAFKDGKPVD
                     GFIGAVPEPMVNEFVDRLLPTDADLVAEEAEQQERSGDAEEAERRYREALDADAGNRS
                     ARLGLGRILSERGDLDEARQTLMPLLPDPDAERTLASITLREWAQDREPGTLASAKRL
                     ASQGRWREALDGMIGALRDDPDARQAMLEVFTVLGDEDPLVVEYRRKLANALF"
     gene            complement(1771..3942)
                     /locus_tag="VE646_08015"
     CDS             complement(1771..3942)
                     /locus_tag="VE646_08015"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZD79971.1"
                     /translation="MEHEVTASERDLAASVKELRLDVMGMTCGSCAARVEKTLNKQPG
                     VRASVNFATGEAVVRLDQGAPALDVLREAVKARGYDIQVHVDAAAEAARREERSWLIR
                     MAVAWPLGIVTMALSMAFMDHTWARWAAFALATPVEFYAGWPFLKGAAVRARALTANM
                     DTLIAVGTMAAYLYSVSVLSGAQDLYFDSSAVIVAFLLLGRWLEARAKGRASQAIRHL
                     LELGAKEARVVRGDKELKVPVDAVKPGWIMRVLPGEKIPTDGRVVDGSSAVDESMLTG
                     ESVPVEKSAGDEVAGATLNTDGMLLVEATRVGTDTALAQIVRLVAEAQGSKAPIQRLA
                     DQVAGIFVPIVMGVALVTAVAWFVLSGGSVHDALLPAVAVLIIACPCALGLATPAAIM
                     VGTGRGAQLGVLIRGGEVLERSRQIDVVVFDKTGTLTEGRMELTDVVGDERTLALAAG
                     AESSSEHPIARAVVAGADRQGVELPAATGFRSVAGRGVRAEVDGVEVLVGRRSLLEER
                     GLLVPAPLADAVVRLEDGGKTVFWAGWGGRARGVLAVADRIKPGAAEAVDALHRLGIE
                     VAMITGDNRATGEAIAAQAGIDRVLADVRPEAKVDEIRRLQAAGKVVAMVGDGINDGP
                     ALAQADLGIAIGTGTDVAIEASDITLIKGDLPGVVRAIRLSRRTFRTIAENLFWAFGY
                     NVVLIPLAALGFLNPIIAGGAMAMSSVSVLTNSLRLKRFKG"
     gene            complement(3951..4232)
                     /locus_tag="VE646_08020"
     CDS             complement(3951..4232)
                     /locus_tag="VE646_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HZD79972.1"
                     /translation="MAGYSNNKQDVLGRLRKIEGQVRGLQRMVEDDKYCIDILTQVNS
                     ATAALKAVGMGLLDEHVRHCVKESIETGQGDEKVEELLAAVARFAGRPS"
BASE COUNT          620 a         1663 c         1338 g          617 t
ORIGIN      
        1 tccctgcggc ctggccgagg agggcgtggc cagcctctcg gggatcgccg gacgaaccat
       61 caccgtggcc gatgccgtcc tgccggtcct ccgtcacttc gagcgagtct tcgacgtccg
      121 gctgcggtcg gcggccgagg aggccgaggc cttcgccgat gcccccctcg cgtcctgagc
      181 gcggtcaggc gtcggccacg aggacgtact ggggcacctg ctcgagccag ccgagcagtg
      241 gcctggaagc gatgcgccgc gcccactcca cgtcccggag gcgccggatc cggctccccc
      301 tccaacccgc ccggtagacc gcgtggatca gcggctccgc cgagggcgac cgcccgagcg
      361 ggagctggga caggatctcg gggtcgtact cggcatggtg gtcgtgcggg atcccgtaca
      421 ggcggaccag gacctcggcg gccctccgct tgacccgctc gggcagatcg tccttgcccc
      481 agatcccctc gaagagcacg agcctgccgc ccggcgccac cgatcccctc caggcccgga
      541 gcgcccgatc cggatccggg agcgtccaca gcatgtgccg ctccatcacc gcgtcgaacg
      601 ggcccggcgg cggctcttcg gccggcccca ccacgaaagt cgccctcacc gcgcgctcag
      661 cggccttgtg ctgagcacgc tcgagcatca gcggtgacag gtccagggcc gtgacctcgt
      721 acccgagctc ggccgccagc atggtcatgg agccggtgcc ggctccaacg tccagaaccc
      781 tggctccccg ggccggcagg gccgcggaca gggccgcgcg ccaggctgcc gcctcgagcg
      841 gatcggaggc tgcgtgcgag ggcgagtggt cgtagacggc cgcgtcggcg tcccaccact
      901 cccggatgcg atcgtggatc tcggccaccg tcgcctcaga ggatcagaac agggcgttcg
      961 ccagcttgcg tcggtactcg accacgagcg ggtcctcgtc gcccaggacc gtgaatacct
     1021 cgagcatcgc ctgccgggcg tccggatcgt cgcgcagggc cccgatcatg ccatccaggg
     1081 cctcccgcca cctgccctgc gaggccaggc gcttggccga ggccagggtg ccaggctccc
     1141 ggtcctgcgc ccactccctc agggtgatgg aggccagcgt gcgctcggcg tcggggtcgg
     1201 gcagcaacgg catcagcgtc tgacgagcct cgtccaggtc tcctcgctcg gacaggattc
     1261 gtcccagtcc gagtctcgcc gagcggttcc ccgcgtcggc gtcgagcgcc tcgcggtacc
     1321 gtcgctcggc ctcctcggcg tcgcccgagc gctcctgctg ctccgcttcc tcggccacca
     1381 gatcggcgtc ggtgggcaac agacgatcga cgaactcgtt caccatgggc tccggaacgg
     1441 caccgatgaa cccgtccacc ggcttcccgt ccttgaacgc gatcaccgtg gggatgctct
     1501 gcacgccgaa ctgcgatgcc gtgtacgggt tcgcgtcgac gtccagcttg gccagcagga
     1561 actgcccgcc gcgctgctcc gccacccgct ccagcatggg accgagcgtc ttgcagggac
     1621 cacaccagga cgcccacaga tccaccacca ccgggcgctg cttcgatccc tcgacgaccg
     1681 tctgcatgaa gtcggcgtcg gtcacatcta cgacggtctg cgttccctgc atcatgtcca
     1741 tctccacatc accctccacc actgtaccgt tcaacccttg aaccgcttga gccggagcga
     1801 gttcgtcagc accgagaccg acgacatcgc catggcgccg ccagcgatga tcgggttcag
     1861 gaaccccagc gccgccagcg ggatcagcac cacgttgtag ccgaacgccc agaacaggtt
     1921 ctcggcgatc gtccggaagg tccggcgcga taggcggatc gcccgcacca cgcccggcag
     1981 gtcacccttg atcaacgtga tgtcggacgc ctcgatcgcg acgtcggtcc cggtgccgat
     2041 cgcgatgccc aggtcggcct gcgcgagggc cggcccgtcg ttgatgccgt ccccgaccat
     2101 ggccaccacc ttgccggccg cctgcagccg gcggatctca tccaccttgg cttcgggccg
     2161 cacgtcggcc aggacccggt cgatccccgc ctgcgccgcg atggcctcgc cggtcgctcg
     2221 gttgtcgccg gtgatcatgg ccacctcgat ccccagccgg tgcaaagcgt ccacggcctc
     2281 ggccgccccg ggcttgatcc ggtccgcgac cgcgagcacc cctcgagccc ggccgcccca
     2341 gcccgcccag aagacggtct ttcctccgtc ctccaggcgg acgaccgcat cggctagtgg
     2401 cgcgggcacg agcagccctc gttcctcaag cagcgagcgg cggcccacca ggacctcgac
     2461 cccgtcgacc tcggcccgta cgccgcgacc ggccaccgaa cgaaacccgg tcgccgccgg
     2521 aagctcgacg ccctgccgat ccgcccccgc caccacggct cgagcgatgg ggtgctcgct
     2581 ggaggactcg gcgccggcgg ccagggccag ggtccgctca tcgcccacca cgtccgtgag
     2641 ctccatccgc ccctcggtca gggtcccggt cttgtcgaac acgacgacgt cgatctgccg
     2701 cgaacgctcc aggacctcgc cgccgcggat cagcacgccc agctgcgccc ctcgcccggt
     2761 cccgaccatg atggcggccg gcgtggccaa gcccagcgcg cacggacagg cgatgatcag
     2821 cacggccacc gccggcagga gcgcatcgtg gacgctgccc ccgctcagaa cgaaccaggc
     2881 caccgccgtg accagcgcca cgcccatcac gatggggacg aagatgccgg ccacctggtc
     2941 ggccaaccgc tggatcggcg ccttggagcc ctgggcctcg gccaccagcc ggacgatctg
     3001 ggcgagcgcg gtgtcggttc ccacccgggt cgcctccacc agcagcatcc cgtccgtgtt
     3061 cagggtggca ccggcgacct cgtcgcccgc agacttctcc accggcacgg actctcccgt
     3121 cagcatggac tcgtccacgg ccgaggagcc atccaccacc cggccgtcgg tggggatctt
     3181 ctcccccggc agcacccgca tgatccagcc gggcttcacc gcgtcgacgg ggaccttgag
     3241 ctccttgtcc ccccggacca cgcgggcctc cttggcaccc aactccagca ggtgcctgat
     3301 ggcctgcgag gctcgtccct tcgctcgcgc ctcgagccac ctgcccagca gcaggaacgc
     3361 cacgatcacg gccgaactgt cgaagtacag gtcctgagcc cccgacagca ccgataccga
     3421 gtacaggtac gcggccatgg tgcccaccgc gatcagcgtg tccatgttcg ccgtgagtgc
     3481 ccgtgcccgg acggcggcac ccttcaggaa gggccagccc gcatagaact cgacgggtgt
     3541 ggccagcgcg aaggccgccc agcgcgccca cgtgtggtcc atgaaggcca tggacaaggc
     3601 catcgtgacg atcccgagcg gccaggccac cgccatccgg atcagccagg aacgctcctc
     3661 gcgacgggcg gcctccgccg ccgcgtccac gtggacctgg atgtcgtagc ctcgcgcctt
     3721 cacggcttcg cgcaggacgt ccagcgccgg ggcgccctgg tccaggcgga ccacggcctc
     3781 cccggtcgcg aagttcacgg aggcacgcac ccccggctgc ttgttcaggg tcttctccac
     3841 acgcgccgcg cacgagccgc aggtcatccc catcacgtcg aggcggagct ccttcacgga
     3901 tgcggccagg tcgcgctccg acgccgtgac ctcgtgctcc atcaggtcgc tcacgatggc
     3961 ctccccgcga accgggcgac ggcggccagg agctcctcca ccttctcgtc gccctggccc
     4021 gtctcgatgc tctccttcac gcagtgccgg acgtgctcgt ccaggagccc catgccgacc
     4081 gccttcagcg cggccgtggc ggagttcacc tgggtcagga tgtcgatgca gtacttgtcg
     4141 tcctcgacca tccgctgcag ccctcggacc tgcccctcga tcttgcggag ccggccgagc
     4201 acgtcctgct tgttgttcga gtacccagcc atgtctcc
//
LOCUS       DASQQQ010000212         2341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_41483, whole genome shotgun sequence.
ACCESSION   DASQQQ010000212 DASQQQ010000000
VERSION     DASQQQ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41483"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VE646_08025"
     CDS             complement(<1..263)
                     /locus_tag="VE646_08025"
                     /inference="COORDINATES: protein motif:HMM:NF014057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose 1,6-bisphosphatase"
                     /protein_id="HZD79973.1"
                     /translation="MPDGPGVRGPLDPGPLPGREGTLKGPEDREAAMKLTLSTIKADT
                     GGFVGHTEVHPEMRQVAEEATAEAVSGGLLLDAHVDRVGDDLAL"
     gene            307..513
                     /locus_tag="VE646_08030"
     CDS             307..513
                     /locus_tag="VE646_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HZD79974.1"
                     /translation="MGVLAQASVPWAALAPLLVAALAFDVYCLIDLARTPVQGLPKWA
                     WVLLILLVNPLGGIAYLITGRAPR"
     gene            510..1397
                     /locus_tag="VE646_08035"
     CDS             510..1397
                     /locus_tag="VE646_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD79975.1"
                     /translation="MNAAVLTEGLGKVYGSTVALDDVGLRVPQGSIYGLVGPNGAGKS
                     TLLGILAGLRRPSAGSVRIDTPPRRIAVMPDTPLFDGWLSAREVVDLARSLTAPETPG
                     TAVGAALSEAGLGDVSDRRVGGFSRGMLQRLGIAATIVGEPELLILDEPCSALDPLGR
                     HEVLELVGRLGRRATVLFSSHILADVQRVCDTVGVLGRGRLLYQGPLDELLGERVVPA
                     YLIRVRGEIAPVMRSLRGVPWVRSVVEEGRGELRAEVTSVAEAERGLMGRLAQTEARV
                     ISVEPAAADLESVFLELTS"
     gene            1394..2140
                     /locus_tag="VE646_08040"
     CDS             1394..2140
                     /locus_tag="VE646_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79976.1"
                     /translation="MSLWKLEWYRLFRTRRWIGLVVVYLLFGLLGPIITRYQEALFRN
                     VGGGIKVIAPPPTVAQAVSSYVSNASQVGLLVVVVIAAGSLGFDARPEWAAFLRSRVP
                     GPATILVPKFTLNAAAAVAAFVLGMLAAWYETVVLIGEVSIPAMIAGMAYGSLYLAFS
                     VAVVALSAALARGVAGVAGLSLAVLIALPILGQVGVMKPWLPSELVGAPVDLVGGGSA
                     ATFLRAAGVSLGATAVALWAAARLLSAREV"
     gene            complement(2161..>2341)
                     /locus_tag="VE646_08045"
     CDS             complement(2161..>2341)
                     /locus_tag="VE646_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HZD79977.1"
                     /translation="CRCRTTTGGASTMEIDPVCGMEVDPATAEWKHEYQGTTYYFCSK
                     GCMEDFAEDPASYVS"
BASE COUNT          267 a          795 c          877 g          402 t
ORIGIN      
        1 agcgccaggt cgtcgcccac ccggtcgacg tgcgcgtcca ggaggagacc cccggagacg
       61 gcctcggccg tcgcttcctc cgccacctgc cgcatctccg gatggacctc cgtgtgcccc
      121 acgaacccac ccgtgtcggc cttgatcgtg gacaacgtga gcttcatcgc cgcctcccta
      181 tcctccggac ccttgagggt cccttcccgg ccgggcagcg ggccagggtc caaggggcca
      241 cggaccccgg gaccatcggg catgggggga gggtgcgggc ccggccccct ccgctaggct
      301 caggacatgg gagtcctggc gcaggcgtcc gtcccctggg cggcgctggc tccattgctg
      361 gtcgccgcgt tggcgttcga cgtctactgt ctcatcgacc tggcccgaac gccagtgcag
      421 gggttgccga agtgggcgtg ggtgctgctc atcctcctgg tcaacccgct cggcggcatc
      481 gcgtacctga tcacaggccg cgcgccccga tgaacgccgc ggtgctcacc gagggcctcg
      541 gcaaggtcta tgggtcgacc gtggctctgg acgacgtcgg gctgcgggtg ccccagggga
      601 gcatctacgg gctggtggga ccgaacggtg ccggcaagag caccctgttg ggcatcctgg
      661 cgggcctccg gcggccctcc gcggggagcg tcaggatcga caccccgcct cggcggatcg
      721 ccgtcatgcc cgacacgccc ctcttcgatg ggtggctctc ggcccgcgag gtggtcgacc
      781 tggcgaggtc cctcaccgca ccggagaccc cgggcaccgc ggtgggggcc gccctgtcgg
      841 aggccggcct gggggacgtg tccgatcggc gggtgggcgg cttctcccgc ggcatgctcc
      901 aacgcctggg catcgcggcg acgatcgtcg gcgagcccga gctgctcatc ctcgatgagc
      961 cctgctcggc cctggacccg ctcgggcgcc acgaggtgct cgagctggtc ggtcggctcg
     1021 gccgccgggc cacggtcctg ttctcgagcc acatcctcgc cgacgtgcag cgcgtgtgtg
     1081 acacggtcgg cgtgctcggt cggggtcgcc tcctgtacca gggcccgttg gacgagctcc
     1141 tgggggagcg ggtggtcccc gcttacctga tccgggtgcg aggggagatc gcgccggtga
     1201 tgcggtcgct gaggggggtc ccgtgggtcc ggtccgtggt ggaggaaggc cggggcgagc
     1261 tccgggccga ggtgacctcg gtcgcggagg ccgagcgggg gctgatgggg cggctggccc
     1321 agacggaggc cagggtgatc agcgtggagc cggcggccgc ggacctcgaa tcggtgttcc
     1381 tggagctgac gtcgtgagcc tgtggaagct cgagtggtac cgcctgttcc ggacccgacg
     1441 atggatcggg ttggtcgtcg tctacctgct cttcgggctc ctcggtccca tcatcacccg
     1501 ctaccaggag gccctgttcc gcaacgtcgg cggcggcatc aaggtgatcg ccccacctcc
     1561 aacggtggcg caggccgtct cctcgtacgt gagcaacgca tcccaggtcg gcctgctggt
     1621 ggtggtggtg atcgccgccg ggtcgctggg gttcgacgcc cgtccggaat gggctgcgtt
     1681 cctgcggagc cgcgtcccgg ggccggccac gatcctggtg ccgaagttca cgctgaacgc
     1741 cgccgccgcg gtggcggcct tcgtcctggg gatgctggcc gcctggtacg agacggtcgt
     1801 gctgatcggg gaggtctcga tcccagccat gatcgcgggg atggcctacg gatcgttgta
     1861 cctggccttc tccgtggcgg tggtggccct gagcgcggcc ctggccaggg gggtggcggg
     1921 cgtggcgggg ctgtcgctgg ccgtcctgat cgccctgccg atcctcgggc aggtgggggt
     1981 gatgaagccg tggctaccca gcgagctcgt cggggccccg gtcgacctgg tcggcggagg
     2041 ctcggccgcc acgttcctcc gggcggcggg cgtgtccctt ggtgcgacgg cggtggctct
     2101 gtgggcggct gctcgactgc tctcggctcg agaggtgtga gccggctcgc ccgtcgaagg
     2161 tcagctcacg taggaggcgg ggtcctccgc gaagtcctcc atgcatccct tcgagcagaa
     2221 gtagtaggtg gttccctgat actcgtgttt ccactcggcg gtcgccgggt cgacctccat
     2281 gccgcagacc gggtcgatct ccatggtgct ggctcctccc gtggtcgttc gacaccggca
     2341 g
//
LOCUS       DASQQQ010000213         4205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_182569, whole genome shotgun sequence.
ACCESSION   DASQQQ010000213 DASQQQ010000000
VERSION     DASQQQ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182569"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VE646_08050"
     CDS             complement(<1..293)
                     /locus_tag="VE646_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018768816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79978.1"
                     /translation="MARRRGFSAFIVVVAVVTLWLAWPSGGGPGRTATGRSGPGNSGR
                     DGAAATSSPSPAGQVGPDSPIKHVVFIVKENRTFNSYFATYPGAEGSTTGGTL"
     gene            complement(413..1837)
                     /locus_tag="VE646_08055"
     CDS             complement(413..1837)
                     /locus_tag="VE646_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZD79979.1"
                     /translation="MPEGRRGSRRAVLAAAAATAVLVGAAAAPHPTPPLPDRRPNVVV
                     ILSDDQTLDTLPHRPAVMPFLQQVMQDPRDHWITFPNAFLNTPLCCPSRATILTGRYS
                     HHTGVRNNSEAIHLDEANTVATWLHDAGYTTGLVGKYLNRYPFHAAPYVPPGWDRWEA
                     KIQGSGASVYYDYVVSRQGFPVAYGHGPEDYSTDVFAGLAEDFIRSAPGDRPFFLLFA
                     PTGPHSPWTPAPRYAGTWSGPVTVPPNLGEPDVSDKPAWVRALPPFTPERRAAAIADR
                     RREYETLGAVDDAVRGLVAALRRRGALEDTVIFYLTDNGESIGEHRWMRKSCPYDACI
                     RTPFLARVPGATARTDDHLVSNVDLAPTIAALAGVRPSRPVDGTSLAPLLGERSAVSW
                     RSGILSEFVGDGTIPAWWEIRTAGAAFIAYATGERELYDLAGLHGPPDPFELRNRAGD
                     PRYTSLEDRLASGLDDLRSGLGSG"
     gene            complement(1843..2616)
                     /locus_tag="VE646_08060"
     CDS             complement(1843..2616)
                     /locus_tag="VE646_08060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZD79980.1"
                     /translation="MAGRPVTRVPEHVRKNRRFWDGHSAEYQREHAPQLNRWDRPVWG
                     IWAIPEDVLGALGDVGGQDVLEFGCGGAQWSIALARRGAHAVGLDLSIEQLRHAWTLM
                     RPAAVRFPVVNGDGEAMPFGDERFDLVFCDHGAMTFGHPFRTVPEAARVLRPGGRFVF
                     NIASVFHSICWNDDLDRVDDRLHRGYFGVRKWDEESVDFQLPYGEWIRLFHANGLEVE
                     DLVELRPPPGARTTYADFVPLDWARRWPAENIWKLRKHP"
     gene            complement(2598..3893)
                     /locus_tag="VE646_08065"
     CDS             complement(2598..3893)
                     /locus_tag="VE646_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor synthesis domain-containing
                     protein"
                     /protein_id="HZD79981.1"
                     /translation="MEPSRRAVVVTVSDGVARGTRTDDSGHQAGELLAAAGFDVSDRL
                     VVPDEREQIRDLLVRLSDVDRVPLVVTTGGTGFGPRDVTPEATREAIEREAPGLVELM
                     RKVGLEQTPMAALSRAVCGSRGATLILNLPGSPRGVSEGLEAVLPVVGHALDLLAGRT
                     GEHPTGHAGGPGHAEVGRWTFAARQAGPFDFTPATERAWVEATAVKVLEGTPPCRVGN
                     RFRVIPEGAVEGTLGCAEFDAAAEADAPKVIALGEPVTRTYRHESGEIEVYLEPHLPP
                     PRLVVVSATETALHLLRLAHALGYQTVVVETRPGRVTHAHRSEAGEEFSAVGDVALDE
                     RTHVVFTDHDAPGLRDMLATLLHSPAGFIGVMGSRRHVGPYLDELRAMGFADPDLARI
                     RSPVGLDLGGREPAEIAISIAAGLVAARHDREGGWLDAR"
     gene            complement(3898..>4205)
                     /locus_tag="VE646_08070"
     CDS             complement(3898..>4205)
                     /locus_tag="VE646_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79982.1"
                     /translation="PGHPDDRRRSLAGAELGGRLQLVALNAGLVLMVLRAGGWVPDVL
                     GAVGVGLALGGAALALLKAWAFNLLGRTPALSDRLLAVWGAAPSGRDRAAGPPGLAG"
BASE COUNT          623 a         1553 c         1470 g          559 t
ORIGIN      
        1 ttcagggtcc cgcccgtcgt ggatccttcc gcccccggat acgtggcgaa gtagctgttg
       61 aaggtacggt tctccttcac gatgaacacc acgtgcttga tgggactatc gggtcccacc
      121 tggccggcgg gagaggggga gctggtcgcc gcggcgccgt cgcggccgga gttcccgggc
      181 ccgctccgac ccgtcgccgt ccgacccggg cctccgccgc tcggccaggc cagccacagc
      241 gtgaccaccg ccacgaccac gatgaacgcg gagaagccac ggcgccgcgc cacctgctgg
      301 cggctgggcc gacggtggtg gcctcgaggc gtggggggtc gagaacccgg gccaggggtg
      361 tactgcaccg cgcaacgata gcagtcgggc gggcggcgcg aggcccgctg gatcagcccg
      421 atccgaggcc ggaccgcagg tcatccaacc cgctcgccag ccgatcctcg agcgaggtgt
      481 agcggggatc cccggcccgg ttcctgagct cgaagggatc gggggggcca tgcagtcccg
      541 ccaggtcgta cagctctcgc tcgccggtcg cgtatgcgat gaacgcggcg cccgccgttc
      601 ggatctccca ccaggccggg atcgtgccgt cccccacgaa ctcggacagg atcccgctcc
      661 gccagctcac cgcggaacgc tcccccagca acggggccag gctcgtcccg tccaccggcc
      721 ggctcgggcg gacgccggcc agcgccgcga tcgtgggcgc gaggtccacg ttggagacca
      781 ggtgatcgtc ggtcctcgcg gtggcacccg gaacgcgggc caggaacggc gtgcggatgc
      841 acgcatcgta cgggcacgat ttgcgcatcc accgatgctc gccgatcgac tcgccgttgt
      901 cggtcaggta gaagatcacc gtgtcctcga gcgctccgcg ccgccggagg gccgcgacga
      961 ggccccgcac cgcgtcgtcc accgctccga gggtctcgta ctcccgccgg cggtccgcga
     1021 tcgcggcggc ccggcgctcg ggcgtgaacg ggggcaaggc tcgaacccac gcgggcttgt
     1081 cggagacgtc gggctcgccc aggttgggcg gcaccgtcac gggaccggac caggtgcccg
     1141 cgtaccgggg cgccggggtc cacggcgagt ggggaccggt gggggcgaac agcaggaaga
     1201 agggtcgatc cccgggagcc gagcggatga agtcctccgc caggccggcg aacacgtcgg
     1261 tcgagtagtc ctctggcccg tggccgtagg cgaccgggaa cccttgcctg gacaccacgt
     1321 agtcgtagta gacgctcgcg cccgagccct ggatcttggc ctcccagcgg tcccatccgg
     1381 ggggcacgta cggggcggcg tggaacggat accggttcag gtacttgccc accagacccg
     1441 tggtgtagcc ggcatcgtgg agccaggtcg cgaccgtgtt ggcctcgtcc aggtggatcg
     1501 cctcgctgtt gttccggacc cccgtgtggt gcgagtaccg cccggtcaag atcgtggccc
     1561 gggaggggca gcacaggggc gtgttcagga aggcgttggg gaaggtgatc cagtgatcgc
     1621 gagggtcctg catgacctgc tgcaggaacg gcatcaccgc cggacgatgt ggcagcgtgt
     1681 ccagcgtctg gtcgtcagag aggatcacga cgacgttcgg ccgacgatcg gggagtggcg
     1741 gtgtggggtg tggagccgcg gccgccccga ccaggacggc cgtggccgcg gccgccgcca
     1801 ggacggctcg acgactgcct cggcgaccct cgggcatggt gctcagggat gcttccggag
     1861 cttccagatg ttctcggccg gccatcgccg cgcccagtcc agcggcacga agtccgcgta
     1921 ggtcgtgcgc gcgcccggcg gtgggcggag ctcgaccaga tcctcgacct ccaggccgtt
     1981 cgcgtggaac aggcggatcc actcaccgta cggcagctgg aaatccaccg actcctcgtc
     2041 ccacttgcgt acgccgaagt acccgcgatg caggcgatcg tccacccgat ccaggtcatc
     2101 gttccagcag atcgagtgga agacgctcgc gatgttgaac acgaaccggc cgcccggccg
     2161 gagcacccgg gccgcctcag gcacggtccg gaacgggtgc ccgaacgtca tcgccccgtg
     2221 gtcgcagaag accaggtcga accgctcgtc accaaagggc atggcctccc cgtccccgtt
     2281 caccaccggg aaccggacgg cagcggggcg catgagcgtc catgcatgcc ggagctgctc
     2341 gatcgacagg tccagcccca cggcgtgcgc tccccggcgt gccagcgcga tcgaccactg
     2401 ggcgccgccg catccgaact cgaggacgtc ctgcccgccg acgtccccca acgcgccgag
     2461 gacgtcctcc gggatcgccc agatccccca gacgggccgg tcccagcggt tcagctgagg
     2521 agcgtgctcg cgctggtact cggcgctgtg cccgtcccag aaccggcggt tcttgcggac
     2581 gtgctcggga acccgggtca ccgggcgtcc agccatccac cctcgcggtc gtgacgagcc
     2641 gcgaccagcc cggccgcgat ggagatcgcg atctcggcgg gctcccggcc gccgaggtcc
     2701 aggccgaccg gcgagcggat ccgcgccaga tccggatcgg cgaaccccat ggcccgcagc
     2761 tcgtccaggt aaggccccac gtgccgacgg ctgcccatca caccgatgaa gccggccggc
     2821 gagtgcagca gcgtcgccaa catgtccctc aggccgggcg catcatggtc cgtgaacacg
     2881 acgtgggttc gctcgtcgag cgctacgtcg ccgacggccg agaattcctc ccccgcctcg
     2941 ctccggtggg catgcgtcac ccggccgggg cgggtctcga ccacgaccgt ctggtagccg
     3001 agtgcgtgag cgaggcgaag caggtggagc gccgtctcgg tcgctgagac caccacgagc
     3061 cgggggggcg gcaggtgcgg ctcgaggtag acctcgatct ccccggactc gtggcggtag
     3121 gtccgggtca ccggctcacc gagcgcgatc accttcggag catcggcctc ggccgcggcg
     3181 tcgaactcgg cgcacccgag cgttccctcc acggctcctt cggggatcac gcggaagcgg
     3241 ttgcccacgc gacacggcgg ggttccctcg aggaccttga cggccgtcgc ctcgacccac
     3301 gctcgttccg tagccggcgt gaagtcgaac ggaccggcct gccgggcggc gaacgtccag
     3361 cggccaacct ctgcgtgacc aggccctccc gcgtgccctg tggggtgctc gccggtccgc
     3421 cccgccagga ggtcgagtgc gtgacccaca accggcagca ccgcctcgag gccctcgctg
     3481 acgcccctcg ggcttcccgg taggttgagg atcagcgtgg cgccgcggct cccgcacacc
     3541 gccctcgaca gcgcagccat cggcgtctgc tccagaccaa ccttccgcat gagctccacc
     3601 aggccgggcg cctcgcgttc gatcgcctcc cgggtggcct ccggcgtcac gtcgcgaggg
     3661 ccgaaccccg tccctccggt ggtcaccacc agcgggaccc gatccacgtc cgacagccgg
     3721 accagcagat cccggatctg ctctcgctcg tcggggacca ccaggcgatc ggacacgtcg
     3781 aaccccgcgg ccgccagcag ctccccggcc tggtgcccgg aatcgtccgt gcgggtccca
     3841 cgggcgaccc catccgaaac ggtgaccacg acggcccggc gggatggttc catgggacta
     3901 tcctgccaga cccggtggcc ccgccgcccg gtcgcgaccc gagggggccg ccccccagac
     3961 ggccaggagc cgatcggaga gcgccggggt cctgcccagc aggttgaagg cccaggcctt
     4021 gagcagggcc agggcggcgc caccgagggc gagacccacc ccgaccgctc cgaggacgtc
     4081 cggcacccag cccccggcgc gtagcaccat gagcaccaga cctgcgttca gggccacgag
     4141 ctggagccgg ccccccagct cggcaccggc cagcgacctc cgccggtcgt ccggatgccc
     4201 cggcc
//
LOCUS       DASQQQ010000214        10385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_154948, whole genome shotgun sequence.
ACCESSION   DASQQQ010000214 DASQQQ010000000
VERSION     DASQQQ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10385
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154948"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..754)
                     /locus_tag="VE646_08075"
     CDS             complement(140..754)
                     /locus_tag="VE646_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79983.1"
                     /translation="MILSLLFALAFIWGVAAMSEHTGGGRTVLLVGTAVGTGISLLGL
                     ILSFVSSKKRHPQTDGQREETNGGAEVSAEGSPQANGSTEEGARTYEEIFGLRTDVPE
                     EGLLGASEGEGWSTDGHDEEEVAAEGPPPTEDQTTPEAGLQPVEPDPDGAADRSDAHD
                     GEVSPDDHLRRMREQFRARAEEAALRVKQREAELQKTASTSDPE"
     gene            complement(1056..1391)
                     /locus_tag="VE646_08080"
     CDS             complement(1056..1391)
                     /locus_tag="VE646_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79984.1"
                     /translation="MRKKEGDAVSLRLDIATVARIEADRIYDLILPEMESEGTVTVPL
                     PFLENVRTTGQKLEPFAPNYIRTALDGLAETAKIRRLRRKAVVPAHTFASVAAAFRIA
                     ADSMEGRTP"
     gene            1621..3042
                     /locus_tag="VE646_08085"
     CDS             1621..3042
                     /locus_tag="VE646_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79985.1"
                     /translation="MNEVSTPEYAPPEPPWATQGSSPFGAVVRGWGTGLIRGGLAFVV
                     MALVGQATAFVVNLGSGGDASIGTVAKLGWLYFGWFHHVAFTTSVVGLRPGSGLAGQA
                     GLDADVGVAMMLATFLAIALLYKGGRAVADRAGGGGVARILHGMKVAPAYAILALLVS
                     ELVIIDIPFPRIGSVSGGLQVRSWGPSWFLLPLLIAVVSGAAGGLRSGRHELLSHDPW
                     GPRAAGAVVGGLRMFMLGLVLPFVGLLVLAGVHPSATKAYFQTISRPPTDQAVAIIAN
                     HALLLPNQSLWVLVPAMGGCDGMTGGGTSASFLCYSKFPGAMTRPEGLRPRTPVVRTN
                     FGRAPLGYFLFLLVPALSAILGGRYAARKRARLQWEAVAVGAASGVVFAVLVVVGSWF
                     ASVSVRSPSTVGGIPGNASVLIGPGLVAGGLLALAWGVVGGGLGGWLEGRKLPSRAEI
                     PRSSGSSAWGAGHTVGSPDVRPG"
     gene            3082..3903
                     /gene="pheA"
                     /locus_tag="VE646_08090"
     CDS             3082..3903
                     /gene="pheA"
                     /locus_tag="VE646_08090"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008632532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HZD79986.1"
                     /translation="MRIAYQGEPGAFSETAVRAVFPDAEAMPCDTVRIAFSRVTSGAV
                     DFGVVPVENSQAGSVNETYDLLLNSSLVRIAGEVVVRVDHALLAVRGTRLEDVRKAYS
                     HWQALAQSEDFLAAHRIEPVPVHDTAGAARLVAGSGKRDEAAIAGVEAAARFGMVVLA
                     ERIQTYPDNFTKFAVIGTRDAGLGPPDKTALVMAVQDRPGSLLASLEPFAGHGINLTK
                     LESRPRRGAPFEYVFYLDIERAEDDPEVLAALEEVRLHTSMLKVLGSYAGSKGPV"
     gene            complement(3949..4848)
                     /locus_tag="VE646_08095"
     CDS             complement(3949..4848)
                     /locus_tag="VE646_08095"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZD79987.1"
                     /translation="MQHDPVETTEACYRHPTQSTRVHCTRCGRPICPDCMIPAPVGFQ
                     CPECVQLARREFRQARPGVARLGLGQGRLTATNLLLLAMGVMFVVEILAGGPNAALDG
                     PTGQRLYDLGALYPPAIASGQYWRLFSAMFLHLGLIHIAFNAYALWLFGQFAERTFGS
                     LVFLLIFFVTGFLASATSYAFGPPLILGAGASGAIFGVFGAFVAYNYRRRHLAMAAAN
                     LRWAVMLIVLNAVLAFGWSGIDWRAHVGGLLAGFAAGFVAEGVGSDQVRRLVRILGFA
                     ALIGVGVALVMTRTADLRSLMGP"
     gene            complement(4912..5322)
                     /locus_tag="VE646_08100"
     CDS             complement(4912..5322)
                     /locus_tag="VE646_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HZD79988.1"
                     /translation="MDRREARSRFELARVARLGSVRPEGGPHLVPVVFAAAGETVYSV
                     VDAKPKRSPHLQRLENARSEPRVSLLVDHYEEDWNALWWVRADGLAVVQTEGPDRDRG
                     IHLLAGKYPRYAERPPDGPALVIRVTRWRWWSAS"
     gene            complement(5342..6307)
                     /locus_tag="VE646_08105"
     CDS             complement(5342..6307)
                     /locus_tag="VE646_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79989.1"
                     /translation="MSLRRGLVLAVVLASVAAACSSGSSGADGRTGGSGAKQAQAFPA
                     SVDLYTGAPQRVAVGLVFANGTLVSFGTVRFAFSYLGSSGSAQATPGPTVTASYVPTP
                     GTPAGGSTPQVTQPSEARGLYEAEDVTFDRAGNWQVQVTADIQGRGTQSATASLQVAD
                     HPALPAPGQPALKTENHTIRSKGVPAAAIDSRAADGGTVPDPELHRWTIAEAVAQHRP
                     ALVVFATPVYCVSRFCGPVTDAVAQLAKRFSNRAVFIHVEIWRNFQKQQINRAAADWL
                     YRNDNLTEPWLYLIGADGTILDRWSSLFSVLEVARDLQQLPPMKR"
     gene            complement(6394..6840)
                     /locus_tag="VE646_08110"
     CDS             complement(6394..6840)
                     /locus_tag="VE646_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD79990.1"
                     /translation="MRHGLARPLPIVVALVALASAGCGSGSEAGSGSSASPLPSGSLG
                     VVEPAAARSAVEALCSISNDETSDRSAANRIFYDQAHEELHVIAAAAQTTDPDTAATL
                     LQAKEQVESDLAAHELPDDFDSDIAHLVGATQEGLRALGLSAPTCG"
     gene            complement(6849..7514)
                     /locus_tag="VE646_08115"
     CDS             complement(6849..7514)
                     /locus_tag="VE646_08115"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZD79991.1"
                     /translation="MNGDLKAMWTAVDVEAAAGRATTELVARASRAGLIDVAVAVVDS
                     PVGSLLIAGTPKGLVRVAFPEEGPEVVMAELAEMLSPRVLEAPSGLDPVRRELDEYFE
                     GRRRRFDLDLDWTLAPGGFSRRVLRYTARIPFGSVSTYGQMARRAGSPRAARAAGNAL
                     HDNPIPIVVPCHRVVPSSGGVGKYGGSEWRKRFLLELEGAVVPVPPNAGRSGHGARGP
                     RLR"
     gene            complement(7511..7999)
                     /locus_tag="VE646_08120"
     CDS             complement(7511..7999)
                     /locus_tag="VE646_08120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZD79992.1"
                     /translation="MSPPPFARFLEEHRVPVYRFLLATLGPQDADDCFQETFLSALRA
                     YPRLRDDSNLRAWIFTIATRKAADHGRSLARRPVPMAELPDRPAPSIEPASAPELWDA
                     VRALPPAQRAAVIQRYVLDLPYAEIGEALGVSEEAARANAYAGRKKLRAGWPRQDREG
                     TG"
     gene            complement(8046..8795)
                     /locus_tag="VE646_08125"
     CDS             complement(8046..8795)
                     /locus_tag="VE646_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HZD79993.1"
                     /translation="MPGDERPHASGEVVPGLDEAIPLLTGGTIRILGLLPRSSNYTFL
                     ARVSEGGREALAVYKPRAGEAPLWDFPEGTLCHREVAAYRVSQALGWPRVPPTVLREG
                     PEGPGSLQLFLEFDPEEHYFTLAERMPDAFRRVALFDVVVNNADRKGGHCLLASDGEL
                     FVVDHGVCFHQHIKLRTVIWEFAGDPIDDELGRDLERLRMDLGDDLRTQLDPLLSARE
                     LEAMRERLDALLRSGTFPRPGPGRPYPWPPV"
     gene            complement(8756..9286)
                     /locus_tag="VE646_08130"
     CDS             complement(8756..9286)
                     /locus_tag="VE646_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HZD79994.1"
                     /translation="MELERADRITAGAIGEPGHRTFYLQARTGGELITVVVEKQQVEL
                     LSASILELLSRIDKETGAGPSDEELEGLEGPVEPRFRVGRLSIGYEEEHDVILLELEE
                     LLEEDEPEADQPASGAEEPERIRLWATREQMLALSRHGAAVAGRGRPTCQFCGNPMDP
                     EGHTCPAMNGHTRPAR"
     gene            complement(9291..9971)
                     /locus_tag="VE646_08135"
     CDS             complement(9291..9971)
                     /locus_tag="VE646_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HZD79995.1"
                     /translation="MTVLLLIRHGTTEASEKGVLAGWMPGVHLTEAGRDQARALVDRL
                     ERVPIAAIYSSPLERCRETASPLAAARGLSVTVRRDLGEVRYGSWTGRKIRDVMRTKR
                     WKVVQGAPSRARFPDGESLLEVQHRAVADLEGLAARHGRRPVAVVTHADVIRLVVSHF
                     AGVHVDLFQRLVIEPGSLSVVSLGEGVPLILKVNETGDPSTLLSRARRQHAGGRRRDG
                     SRSPKVRG"
     gene            complement(10030..>10385)
                     /locus_tag="VE646_08140"
     CDS             complement(10030..>10385)
                     /locus_tag="VE646_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZD79996.1"
                     /translation="PIRGSGRDLSLLVRNLIDNAVHYTKPGGSVSVSVAAAQCGVVIT
                     VSDTGIGIPSRDLPRIFERFYRVDRARSRDTGGTGLGLSIVKHVSENHGGTIEVRSEL
                     GRGTTVTVDLPSGSA"
BASE COUNT         1495 a         3705 c         3615 g         1570 t
ORIGIN      
        1 cggttccgct ccgagtgcat tatggccgtg gacaggacga ggccggcgac gaacagcaac
       61 gagaggaccg cccagaccca ccgtcgctca cgccggccgc tctggatctt cgccatctcc
      121 cggatcctcc gctcgcctgt cactcgggat ccgacgtgga cgcggtcttc tggagctcgg
      181 cctcccgctg cttgacgcgg agggcggcct cctcagccct ggccctgaac tgctcacgca
      241 tccggcggag gtggtcgtcg ggtgacacct caccatcgtg agcatcgctt cgatcggccg
      301 ctccatcggg atcgggctcg acgggttgga gccccgcctc gggggtggtc tgatcctccg
      361 tcggaggcgg cccttcagcc gctacctctt cctcatcgtg gccatcggtc gaccatccct
      421 caccttctga tgccccgagg agtccttcct cggggacatc ggttcggaga ccgaagatct
      481 cctcgtaggt gcgagcccct tcctccgtcg acccgttcgc ctgcggtgac ccttcggcgc
      541 tcacctctgc gcccccgttg gtctcctccc gctgcccgtc cgtctgcggg tgacgcttct
      601 tggacgagac gaacgagagg atgaggccca gcagagagat gccggttccg accgccgttc
      661 cgaccagaag gacggtccga ccaccgcccg tgtgctcgct catggcggcg accccccaga
      721 tgaaagccag cgcgaacagc aacgacagga tcacccatcg gcgccgtcgc tgatgtccgg
      781 caacgtgtct acacatcgct aaccccccaa cgatcgatgg gcccgacgct catatcggcg
      841 cggaggcggg ggccgtgtat ccgtcctacg gactatttgt ccagcccgat gcgtccgtcg
      901 aagcagcggc cgcgctcacc tggtcggcct acaacacggg cctcgggagg cccgatggag
      961 cgctgcggcg ttggctcctt ccgtatggat gctccttctg gcctgggacc atccaggggt
     1021 gccaccgtgg ccgagctacc gttcctcatg ccgactcatg gggtccgccc ctccatggag
     1081 tccgctgcga tccggaacgc ggccgccacc gatgcgaagg tgtgcgcagg gacgacggcc
     1141 ttcctcctca gccggcgtat cttcgccgtc tccgccagtc catccagcgc cgtcctgatg
     1201 tagttcgggg cgaacggctc cagcttctgt cccgtggtcc gcacgttctc gaggaacggg
     1261 agtggcaccg tcaccgtccc ctcgctctcc atctccggga ggatcaggtc gtagatccga
     1321 tccgcctcga tcctggccac cgtggcgatg tccaaacgca ggctcaccgc gtctccctcc
     1381 ttcttcctca tcccttcacg ctcctatcag tctcggcgtg ctgcccgccc aacctgaccg
     1441 gggagcgcga acgtcgccgc cctttcgagc gacaccacct caagaacgca agggatcgag
     1501 acgatgaggc ggatggatga cggatcggcg tggagctcag ccgatgggcg ggcctcatcc
     1561 gttccggatc cgggggacgc gcgatcttgg agggaaccca tgctccacca gcgatctgag
     1621 gtgaacgagg tctcgacgcc cgaatacgct ccgccggagc ctccctgggc gacccaggga
     1681 tcgtcgccgt tcggcgccgt cgttcggggg tggggcaccg ggctgatccg cggcgggctg
     1741 gccttcgtgg tgatggccct cgtggggcag gcgacggcgt tcgtcgtgaa cctgggcagc
     1801 gggggcgacg cgtcgatcgg aaccgtcgcc aagctgggtt ggctgtactt cgggtggttc
     1861 catcacgtag cgttcacgac ctcggtcgtc gggctgcgtc cgggatctgg gctggccggc
     1921 caggccggcc tcgatgccga cgtcggggtc gcgatgatgc tcgccacgtt cctggcgatc
     1981 gcgctcctgt acaagggcgg gcgtgccgtg gccgatcgcg cggggggtgg gggtgtggcc
     2041 cggatcctcc acgggatgaa ggtggcgccg gcgtacgcga tccttgccct cctggtatcc
     2101 gagctcgtga tcatcgacat ccccttcccg cggatcggat cggtgtcggg cggcctccag
     2161 gtgcggtcgt ggggaccatc gtggttcctg ctccccttgc tgatcgcggt cgtgtcgggc
     2221 gccgccggcg gtctgcgctc cggccgccac gagctcctct cacacgatcc gtggggtcca
     2281 cgcgcggccg gcgccgtggt gggggggctg cggatgttca tgctcggcct ggttctgccc
     2341 ttcgtcgggc tcctcgtgct ggcgggggtc cacccaagcg cgaccaaggc ctatttccag
     2401 accatctccc ggcctcccac cgatcaggca gtggcgatca tcgcgaacca cgccctgctg
     2461 ctgccgaacc agtcgctgtg ggtgttggtc ccggcgatgg ggggctgcga tggcatgacc
     2521 ggaggaggca cctccgcctc gttcctctgc tactcgaagt tcccgggagc gatgacgcgc
     2581 cctgaaggtc ttcgcccccg cacaccggtc gtccgaacga acttcgggag ggcaccgctc
     2641 ggctacttcc tcttcctcct ggtcccagcg ctgtcggcga tcctgggagg ccgatacgct
     2701 gcccggaagc gcgcccggct ccagtgggag gccgtggcgg tgggagccgc gtccggcgtg
     2761 gtgttcgcgg tcctggtggt ggttggctcg tggttcgctt cggtgtcggt ccgctctccc
     2821 tccaccgtgg gaggcatccc cggaaacgcc tcggtcctga tcggacccgg gttggtggcg
     2881 ggcgggctgc tggccctggc gtggggcgtc gtcgggggcg gattgggggg atggctggag
     2941 ggcaggaagc tcccaagccg ggcagagatc ccgcgatcgt cgggctcctc cgcgtggggg
     3001 gccgggcata ccgtcggctc gcctgacgtg cgccccggct gagggcgtcg ggtctcgatg
     3061 cgctgctagg cttcaccggc gatgaggatc gcctaccagg gggagccggg cgcgttcagc
     3121 gagacggccg tgagggccgt ctttcccgac gccgaggcga tgccgtgcga cacggtccgg
     3181 atcgccttct cccgggtcac cagcggagcg gtcgacttcg gcgtggtccc ggtcgagaac
     3241 tcccaggccg gctcggtgaa cgagacctac gacctgctcc tgaacagcag cctggtgcgg
     3301 atcgcggggg aggtggtggt gcgcgtcgac cacgcgctcc tcgcggttcg gggcacccgc
     3361 ctggaggacg tccgcaaggc ctactcgcac tggcaggcgc tcgcccagag cgaggacttc
     3421 ctggccgccc accgcatcga gccggttccg gtccacgaca cggccggggc cgctcggttg
     3481 gtggccggat ctggcaagcg ggacgaggcg gcgatcgccg gcgtcgaggc ggccgcccgg
     3541 ttcggcatgg tggtgctggc cgagcgcatc cagacgtacc cagacaactt cacgaagttc
     3601 gccgtgatcg gcacgaggga cgcggggctc gggccccccg acaagaccgc cctggtgatg
     3661 gcggtgcagg accggccggg atcgctgctg gcctctctgg agccgttcgc cggccacggg
     3721 atcaacctga cgaagctcga gtcgcgcccc cgccgcggtg ccccgttcga gtacgtcttc
     3781 tacctggaca tcgagcgggc ggaggacgat ccggaggtgc tcgccgccct cgaggaggtg
     3841 cggttgcata cctcgatgct caaggtcctg ggttcgtacg cgggctccaa gggccccgtg
     3901 tagcgaggcc ggcctgccgc ggctttcgag ggcgtcaggg atgaggccct acgggcccat
     3961 cagcgaccgg aggtctgcag tgcgggtcat gaccagggcc acaccaaccc cgatcagcgc
     4021 cgcgaagccc aggatccgga ccaggcggcg gacctggtcc gagcccacgc cctcggccac
     4081 gaagcccgcg gcgaagccgg ccagcaggcc accgacgtgg gcccgccagt cgatgcccga
     4141 ccagccgaac gcgagcaccg cgttcagcac gatcagcatg accgcccagc ggaggttcgc
     4201 cgccgccatc gccaggtgcc ggcgtcggta gttgtaggcc acgaatgcac cgaacacgcc
     4261 gaagatcgct cccgaggctc cggcccccag gatcagcggg ggcccgaacg cgtacgacgt
     4321 ggccgaggcc aggaaccccg tcacgaagaa gatcagcagg aagaccaggg atccgaaggt
     4381 ccgctcggcg aactgaccga acaaccacaa ggcgtaggcg ttgaaggcga tgtggatcag
     4441 gcccaggtgg aggaacatcg ccgagaacag ccgccagtac tgcccggaag cgatggccgg
     4501 cgggtacagg gcccccaggt cgtacaggcg ttgcccggtc ggcccgtcga gcgccgcgtt
     4561 cgggccaccg gccaggatct ctaccacgaa catcacgccc atggccagga gcaggaggtt
     4621 ggtcgcggtc aggcggccct gaccgaggcc gaggcgcgcc acccccggcc gggcctggcg
     4681 gaactcgcgc cgggccagct gcacgcactc ggggcactgg aagcccacgg gagcggggat
     4741 catgcagtcc gggcagatgg ggcgaccgca gcgcgtgcag tgcacgcggg tggactgcgt
     4801 cgggtgccgg tagcaggcct cggtcgtctc cacggggtcg tgctgcatgc cagccatgct
     4861 acgggtcggg gcggggagcg acccgtcggc cggtgctcga aggtggggga ctcagctcgc
     4921 cgaccaccat cgccaacggg tgacccggat caccagggcc ggaccatcgg gcggacgctc
     4981 agcgtaccgg gggtacttgc cggccaggag gtggatcccg cggtcgcggt cgggtccttc
     5041 cgtctgcacg acggccaggc cgtcggcgcg gacccaccac agggcgttcc agtcctcctc
     5101 gtagtggtcc accagcaggc tcacccgagg ctcggaccgg gcgttctcca gtcgctgcag
     5161 atgcggcgag cgcttcggct tggcatcgac cacggagtac acggtctcgc cggccgccgc
     5221 gaacaccacg ggcaccaggt gagggcctcc ctcgggacgg acggagccca gtcgggccac
     5281 cctggccagt tcgaaccggc tcctcgcctc ccgtcgatcc atcgggacac ggaccccgat
     5341 ctcagcgctt catggggggt agctgctgga ggtcgcgagc gacctccagg acgctgaaca
     5401 ggctcgacca gcgatccagg atcgtgccgt cggcgccgat caggtacagc cacggttcgg
     5461 tcaggttgtc gttgcggtac agccagtccg ccgccgcccg gttgatctgc tgcttctgga
     5521 agttgcgcca gatctccacg tggatgaaga cggcacggtt cgagaagcgc ttcgcgagct
     5581 gcgccacggc gtccgtcacc ggtccgcaga accggctcac gcagtacacg ggcgtggcga
     5641 agaccaccag ggcgggacgg tgctgtgcga cggcctccgc gatcgtccat cggtggagct
     5701 cgggatcggg cacggttccg ccgtccgcgg cccgggaatc gatcgccgcg gcggggaccc
     5761 ccttcgagcg gatcgtgtga ttctccgtct tcagcgcggg ctgtccgggt gcgggcagcg
     5821 cgggatggtc cgccacctgg aggctcgcgg tggcgctttg cgttccgcgg ccctggatgt
     5881 cggcggtgac ctggacctgc cagttcccgg cccgatcgaa ggtcacgtct tcggcctcgt
     5941 agaggccgcg ggcctccgag ggctgggtca cctgcggcgt cgacccaccc gccggcgtac
     6001 cgggggtggg cacgtacgac gccgtcaccg tgggtcctgg ggtggcttgc gcggacccgg
     6061 agctacccag gtaggagaag gcgaaccgga cggtcccgaa gctgaccagc gtgccgttgg
     6121 cgaacaccag ccccaccgcc acccgctgcg gcgcaccggt gtacagatcg acactggcgg
     6181 ggaaggcctg cgcctgcttc gcccccgacc cccccgtgcg gccgtccgca ccggacgatc
     6241 ccgacgagca cgccgccgct accgatgcca ggacgacggc gagcaccagt ccccgtcgaa
     6301 ggctcatgct ccctatcatc tcgcccgacc tcggctccgt caggctcgag gcgcgaccat
     6361 gggctcggtg ggccaaggag cggagtcccg acctcagccg cacgtcgggg ccgagagccc
     6421 cagcgcgcgc aggccctcct gggtcgcccc gaccagatgc gcgatgtccg aatcgaagtc
     6481 gtccggcagc tcgtgggccg ccaggtcgct ctccacctgc tccttggcct ggaggagcgt
     6541 ggcggcggta tccggatccg tggtctgggc ggcggcggcg atgacgtgca gctcctcgtg
     6601 agcctggtcg tagaagatcc ggttggccgc cgagcggtcc gaggtctcgt cgttggagat
     6661 gctgcacagg gcctcgaccg ccgatcgtgc ggccgccggc tccaccaccc caagcgagcc
     6721 cgagggcagg ggggaagccg aggatccgga ccctgcctcc gagccggagc cgcagcccgc
     6781 ggatgcgagc gccacgagcg ccaccacgat gggtaggggg cgagcgagac cgtgacgcat
     6841 cgcccccatc atcgcagacg aggtccacga gcgccgtgtc ccgatcgacc ggcgttgggc
     6901 ggcaccggaa cgaccgcgcc ctcgagctcg aggaggaagc gcttgcgcca ctcgctccca
     6961 ccgtacttcc cgaccccacc cgacgacggc accacccggt ggcagggaac gacgatcggg
     7021 atcgggttgt cgtgcagggc gttgccggcg gcccgggccg cccggggact cccggcccgc
     7081 cgggccatct gcccgtaggt ggagaccgat ccgaacggga tccgggccgt gtatcgcagc
     7141 acgcgtcgcg agaagccacc cggagccagg gtccagtcca agtcgagatc gaagcgcctc
     7201 cggcgtccct cgaagtactc gtcgagctcc cgccggaccg ggtcgaggcc cgagggcgcc
     7261 tcaaggacgc ggggcgaaag catctcggcc agctcggcca tcaccacctc gggaccttcc
     7321 tcggggaagg ccacgcggac caggcccttc ggggtcccgg cgatcagcag gctgcccacc
     7381 ggagagtcca cgacggccac cgccacgtcg atcagccctg cccgagaggc gcgcgccacc
     7441 agctcggtgg tggcgcggcc ggcggccgcc tcgacgtcca cggccgtcca catcgccttc
     7501 aggtcaccgt tcatcccgtt ccctcccgat cctggcgcgg ccaacccgct cggagcttct
     7561 tcctgccggc gtaggcgttc gccctagccg cctcctcgct cacccccagg gcttcgccga
     7621 tctccgcgta gggaaggtcg agcacgtacc gctggatcac cgcggcccgc tgggccggcg
     7681 ggagggctcg aaccgcgtcc cagagctccg gggccgaggc cggctcgatc gagggcgcgg
     7741 gtcggtcggg gagctccgcc atcgggaccg gtcgccgggc cagggaccga ccgtggtccg
     7801 ccgccttccg ggtggcgatc gtgaagatcc aggctcgcag gttggagtca tcgcggagcc
     7861 gcgggtacgc ccggagcgcc gagaggaagg tctcctggaa gcagtcgtcg gcatcctgcg
     7921 ggccgagcgt ggcgagcagg aaccggtaga ccggcacgcg gtgctcctcc aggaaccgag
     7981 cgaagggtgg tgggctcatg tccacccagg agaacggccg agggggccga tccgtgagac
     8041 gggactcaca ccggcggcca tgggtagggg cgtccgggcc ccggccgggg gaacgtcccc
     8101 gagcgcagga gcgcatccag ccgctcccgc atcgcctcga gctcccgcgc cgacaggagc
     8161 ggatcgagct gtgtgcgcag gtcgtccccc agatccatcc gtagtcgctc gaggtcccgc
     8221 ccgagctcgt cgtcgatggg gtcaccggcg aactcccaga tcaccgtgcg gagcttgatg
     8281 tgctggtgga agcacacccc atgatccacc acgaacagct cgccgtcgga cgcgagcagg
     8341 cagtgacccc ccttccggtc ggcgttgttc accaccacgt cgaacagggc cacccgccgg
     8401 aaggcgtccg gcatccgctc cgccagcgtg aagtaatgct cctcggggtc gaactccaag
     8461 aagagctgca gggagcccgg tccctccgga ccctcgcgga ggaccgtggg gggcacccgt
     8521 ggccagccga gggcctggga gacgcggtaa gcggccacct cgcgatgaca cagggttccc
     8581 tcggggaagt cccagagcgg cgcttccccg gcccggggtt tgtacacggc cagggcctcc
     8641 cgtccccctt cgctcacccg ggccaggaac gtgtagttgg aggaccgggg caggagcccc
     8701 aggatccgga tcgtccctcc ggtcagcagg gggatggcct cgtcgaggcc ggggactacc
     8761 tcgccggacg cgtgtggccg ttcatcgccg ggcacgtatg gccctccggg tccatcgggt
     8821 tgccgcagaa ctggcaggtg ggccgccccc gcccggccac ggccgccccg tgacgggaca
     8881 gggcgagcat ctgctcgcgg gtcgcccaca ggcggatccg ttccggttcc tccgcgcccg
     8941 aggcgggctg gtcggcctcg ggctcatcct cctcgaggag ctcctccagc tcgaggagga
     9001 tgacgtcgtg ctcctcttcg tagccgatcg agagccggcc cacccggaag cggggctcca
     9061 ccggcccctc cagcccttcc agctcctcat cgctgggtcc ggctccggtc tccttgtcga
     9121 tgcgggacag cagctcgagg atcgaggcgg acagcagctc cacctgctgc ttctcgacca
     9181 ccaccgtgat gagctccccg ccggtccggg cctggaggta gaaggtgcga tgtccgggct
     9241 caccgatggc gccggccgtg atccggtccg cgcgttccag ctccaccgcc ctatcctcgc
     9301 accttcgggg accggctccc atcccgcctc cggccgcccg catgttggcg tcgagcccgg
     9361 ctcaggaggg tggaggggtc gcccgtctcg ttcaccttca ggatcagggg aacgccttcc
     9421 ccgagggaca ccacggagag ggagccgggc tcgatcacca gtcgctggaa caggtccacg
     9481 tgcaccccgg cgaagtgcga gacgaccagc cggatgacgt cggcgtgggt caccacggcc
     9541 accggacgcc gcccgtggcg agcggcgagg ccctccaggt cggccacggc gcggtgctgc
     9601 acctcgagca ggctctcgcc gtccggaaac cgggcccgcg acggcgcacc ctggacgacc
     9661 ttccaccgct tcgtgcgcat gacgtcccgg atcttccgcc ccgtccagct cccgtagcgg
     9721 acctctccca ggtctcggcg taccgtgacg ctgaggcccc gagcggcggc caggggggag
     9781 gcggtctcgc ggcaccgctc cagcgggctc gagtagatcg cggcgatcgg cacccgctcc
     9841 agacggtcca ccagggctcg tgcctggtcc cgacctgcct cggtcaggtg cacgcccggc
     9901 atccatccgg cgagcacccc cttctcgctg gcctcggtgg tgccgtgccg gatcagcagc
     9961 agcacggtca tcggggcagg atagcggccc tcggcatccg tctgcttcgc cggtgaagtc
    10021 ctctcgctct cacgccgacc cggagggcag atcgaccgtc acggtggtcc cacgacccaa
    10081 ctcgctccgc acctcgatcg tccccccatg gttctcgctc acgtgcttga cgatggacag
    10141 ccccaagccg gtcccgcccg tgtcccgcga tcgggctcgg tcgacccggt agaagcgctc
    10201 gaagatcctg ggcaggtccc ggctggggat cccgatgccc gtatcgctca cggtgatgac
    10261 cactccacac tgggcggccg caaccgagac cgagacgctg cctcccggct tcgtgtagtg
    10321 gaccgcgttg tcgatcaggt tgcgcacgag cagggacaga tcccgacccg agccgcggat
    10381 cggtg
//
LOCUS       DASQQQ010000215         2879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_289673, whole genome shotgun sequence.
ACCESSION   DASQQQ010000215 DASQQQ010000000
VERSION     DASQQQ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289673"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /gene="rpsH"
                     /locus_tag="VE646_08145"
     CDS             complement(<1..287)
                     /gene="rpsH"
                     /locus_tag="VE646_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZD79997.1"
                     /translation="MVSTKLRTNVTDPVADLLTRIRNANLAYKDDLLVPSSKMNRAIL
                     GILEAEGYIGGFEPEGEGIHQAFRVRLKYGAKRERTITGLRRISKPGRRVYA"
     gene            complement(281..466)
                     /locus_tag="VE646_08150"
     CDS             complement(281..466)
                     /locus_tag="VE646_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HZD79998.1"
                     /translation="MAKKALINKQERPAKFQVREYTRCRRCGRSRAVYRKFLLCRICL
                     RELAHAGELPGVTKASW"
     gene            complement(478..1029)
                     /gene="rplE"
                     /locus_tag="VE646_08155"
     CDS             complement(478..1029)
                     /gene="rplE"
                     /locus_tag="VE646_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HZD79999.1"
                     /translation="MRYREELVPRLQAELGLANRMQVPGLEKIVVNMGIGDALKDGRM
                     LEAAVEDLTVITGQKPVVTKARKSIAGFKLREGMSIGAKVTLRGDRMWEFLDRLLSTA
                     IPRIRDFRGLSPQAFDGHGNYTLGLTEQLIFPEIDYDKVAKVRGMDITVVTTAVDDER
                     GRALLQALGFPFEGMPAVTAAAS"
     gene            complement(1104..1442)
                     /gene="rplX"
                     /locus_tag="VE646_08160"
     CDS             complement(1104..1442)
                     /gene="rplX"
                     /locus_tag="VE646_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HZD80000.1"
                     /translation="MPGVDIKKDDTVRVMTGKSRGHTGRVIRVVPDKGRVLVEGAALA
                     KKHQRTQGRRSTGGQQLEQGGIIDTELPIDISNVQIVCRSCGQPTRVGHQVNADGTKS
                     RICRKCGAEL"
     gene            complement(1442..1813)
                     /gene="rplN"
                     /locus_tag="VE646_08165"
     CDS             complement(1442..1813)
                     /gene="rplN"
                     /locus_tag="VE646_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HZD80001.1"
                     /translation="MIQQETRCRVADNTGAKEVLVIRVLGGSHRRYAGIGDVVVGSVK
                     DALPNAGVKKGDVVKAVVVRTAKERRRPDGSYIRFDDNAVVLLATDTRNPRGTRIFGP
                     VARELRDKRFMKIISLAPEVL"
     gene            complement(1823..2080)
                     /gene="rpsQ"
                     /locus_tag="VE646_08170"
     CDS             complement(1823..2080)
                     /gene="rpsQ"
                     /locus_tag="VE646_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HZD80002.1"
                     /translation="MAERGRRKVRTGVVVSDKMDKTVLVKVDRTIRHPLYKKIVRRSS
                     KLAAHDETNDARMGDTVRVMETRPVSKTKRWRVVEIVERAK"
     gene            complement(2095..2370)
                     /gene="rpmC"
                     /locus_tag="VE646_08175"
     CDS             complement(2095..2370)
                     /gene="rpmC"
                     /locus_tag="VE646_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HZD80003.1"
                     /translation="MTRIQELRDLEDVDLLERLESNKEELFNLRFQLATGQLDNPMRI
                     KQVRHDVARILTILRERDLAAELDNSVVEETAVEDLEVQEPQAEEES"
     gene            complement(2367..2786)
                     /gene="rplP"
                     /locus_tag="VE646_08180"
     CDS             complement(2367..2786)
                     /gene="rplP"
                     /locus_tag="VE646_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HZD80004.1"
                     /translation="MLAPKRVRHRKVHRGRMRGQAKGGSEVRFGDYGLVALEPAWITN
                     RQIESARVAITRHIRRGGKVWINIFPDKPYTKKPAETRMGSCKGNPEGWVAVVKPGRV
                     MFELAGVPESLAREAMLRAAHKLPIKTKFVARVGGAT"
BASE COUNT          369 a         1093 c          918 g          499 t
ORIGIN      
        1 gcgtacaccc gccggcccgg cttggagatg cgacgcaggc cggtgatcgt gcgctcgcgc
       61 ttggccccgt acttcagccg gacccggaag gcctggtgga tcccctcgcc ctcgggctcg
      121 aagccgccga tgtagccctc ggcctccagg atgccgagga tcgcccggtt catcttcgac
      181 gagggcacca gcagatcgtc cttgtacgcc aggttggcgt tgcggatccg ggtcagcagg
      241 tcggcgaccg ggtcggtgac gttcgtgcgc agcttcgtgc tcaccatgag gccttcgtca
      301 cccccggcag ctcccccgcg tgggccagct cgcgcaggca gatccggcac agcaggaact
      361 tgcggtacac ggcgcgcgag cgcccgcacc gccggcaccg ggtgtactcg cgcacctgga
      421 acttcgccgg ccgttcctgc ttgttgatca atgccttctt cgccatgcca ccaaccccta
      481 cgacgccgcc gccgtgaccg cgggcatccc ctcgaagggg aagcccagcg cctgcagcag
      541 cgcgcgtccc cgctcgtcgt ccacggcggt ggtcaccacc gtgatgtcca tcccccggac
      601 cttggccacc ttgtcgtagt cgatctcggg gaagatcagc tgctcggtca ggccgagcgt
      661 gtagttgccg tgcccgtcga aggcctgggg cgacaggccc cggaagtcgc ggatccgcgg
      721 gatcgccgtg gacagcagcc ggtccaggaa ctcccacatc cggtcgcccc gcagggtgac
      781 cttggccccg atcgacatgc cctcgcggag cttgaacccg gcgatcgact tgcgggcctt
      841 ggtcaccacc ggcttctgcc cggtgatcac ggtgaggtcc tccaccgcgg cctccagcat
      901 ccggccgtcc ttcagggcgt ccccgatgcc catgttcacc acgatcttct ccagccccgg
      961 cacctgcatc cggttggcca ggcccagctc ggcctgcagc cgcggcacca gctcctcgcg
     1021 gtagcgcacc ttgagccggg gccggtagcc gtcgctccgc tggccggccg gcctgcgctc
     1081 gcccgtggcc ttgccgctct ggctcacagc tccgctccgc acttgcggca gatccgggac
     1141 ttcgtgccgt cggcgttcac ctggtggccc acccgggtgg gctggccgca ggagcggcac
     1201 acgatctgca cgttcgagat gtcgatgggc agctcggtgt cgatgatgcc gccctgctcg
     1261 agctgctggc caccggtgga gcgccgaccc tgcgtgcgct ggtgcttctt ggccagggcg
     1321 gcgccctcca ccagcacccg gcccttgtcc gggaccacgc ggatcacgcg cccggtgtga
     1381 ccacggctct tgccggtcat cacccgcacg gtgtcgtcct tcttgatgtc cacgcccggc
     1441 atcacagcac ctccggtgcc aacgagatga tcttcatgaa ccgcttgtcg cggagctcgc
     1501 gcgccacggg cccgaagatg cgggtgccgc gcgggttccg cgtgtcggtg gccagcagca
     1561 ccaccgcgtt gtcgtcaaac ctgatgtagg aaccgtcggg ccgacgccgc tccttcgcgg
     1621 tgcgcaccac caccgccttg acgacgtcgc ccttcttcac gcccgcgttc ggcagggcgt
     1681 ccttgacgga gcccaccacc acgtcgccga tcccagcgta cctgcgatgg gacccaccca
     1741 gcacgcggat caccaggacc tccttggccc ccgtgttgtc cgccacccgg cagcgcgtct
     1801 cctgctggat cacccctgct ccctacttgg cccgttccac gatctccacc acccgccacc
     1861 gcttggtctt ggagaccggc cgggtctcca tcacccgcac ggtgtcgccc atgcgcgcgt
     1921 cgttcgtctc gtcgtgggcg gcgagcttgg acgagcggcg gacgatcttc ttgtacaggg
     1981 ggtgccggat ggtgcggtcc accttgacca gcacggtctt gtccatcttg tcggacacca
     2041 ccaccccggt ccggaccttg cgccggcccc gctccgcgat cgctccgctc tggctcacga
     2101 ttcctcctcc gcctggggct cctgcacctc gaggtcctcg accgccgtct cctcgaccac
     2161 cgagttgtcg agctcggcgg ccaggtcgcg ctcgcgcagg atcgtgagga tccgggccac
     2221 gtcgtggcgg acctgcttga tgcgcatggg gttgtccagc tgcccggtgg ccagctggaa
     2281 ccggaggttg aacagctcct ccttgttcga ctccaaccgc tcgagcaggt cgacgtcctc
     2341 gaggtcccgc agctcctgga tccgggtcac gtcgcacctc cgacccgggc gacgaacttg
     2401 gtcttgatgg gcagcttgtg cgcagcccgc agcatggcct cgcgggccag ggactcgggc
     2461 accccggcca gttcgaacat cacccgcccc ggcttgacca cggccaccca gccctcgggg
     2521 ttgcccttgc acgagcccat ccgggtctcg gcgggcttct tcgtgtaggg cttgtcgggg
     2581 aagatgttga tccacacctt ccccccgcgg cggatgtgcc gggtgatggc cacacgggcg
     2641 gactcgatct ggcggttcgt gatccaggcc ggctccaggg ccaccaagcc gtagtcgccg
     2701 aagcggacct cgctgccgcc cttggcctgg ccgcgcatcc ggccccgatg caccttgcgg
     2761 tgacggaccc gcttgggagc gagcatcacg caccctcctc ggagctcggg ggggcctgct
     2821 cgccatccgt cgacgcgggc gtctccggcg cgggcgtctc cggcgcgggc gtctccggc
//
LOCUS       DASQQQ010000216         7708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_182715, whole genome shotgun sequence.
ACCESSION   DASQQQ010000216 DASQQQ010000000
VERSION     DASQQQ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7708
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182715"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..73
                     /locus_tag="VE646_08185"
     CDS             <1..73
                     /locus_tag="VE646_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80005.1"
                     /translation="GLRFAIREGGRTVGSGRVTKIIK"
     gene            83..250
                     /gene="rpmG"
                     /locus_tag="VE646_08190"
     CDS             83..250
                     /gene="rpmG"
                     /locus_tag="VE646_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015618978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HZD80006.1"
                     /translation="MAKKEGNRPKITLECTECRRRNYHTVKNRINDRERLELRKYCRW
                     ERRYTLHRETR"
     gene            complement(247..966)
                     /locus_tag="VE646_08195"
     CDS             complement(247..966)
                     /locus_tag="VE646_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD80007.1"
                     /translation="MPSNDHKRIKVLIVDDHRTFGEALQLALGREKDLRVVDVVTDAP
                     SVIEAVRLHDPDVILMDMARPDAQGIETTRRIKDADPDAHVLILSGHEDEYLLARVVQ
                     AGATGLLRRSEGLTLVASSVRRVHRGEPLHDREEIEGALRRLRHRRDREASTEQRLAR
                     LTPREVEILQRMASGGPSDRMAKDLGMSPHTLRTHTQNIITKLGVHSRLEALVLAIRH
                     GTVTMLDVTDDREDWPSRGEP"
     gene            1056..1862
                     /locus_tag="VE646_08200"
     CDS             1056..1862
                     /locus_tag="VE646_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZD80008.1"
                     /translation="MGRLEGKVAVITGAGRGIGRATAIRFAAEGAAVVVNDVDADPAR
                     GTAATIREAGGRAAVSTDDTVELEGGRALVDLAVSEFGGLDILVNNAGITRDRMFHKL
                     DDAQWDLVLDVNLRTAFHATLAASAHMRESAKRETAERGTPLYHRKLTFTTSYSALAG
                     NVGQANYTASKGAIIGLMRTLARELGPFRINVNAVAPGFIETRLTAAKSEQDELGIPE
                     HLRQMALMTIALGRYGRPEDVAGVHLFLASEDADYVTGVVLPVTGGQLGS"
     gene            1868..2266
                     /locus_tag="VE646_08205"
     CDS             1868..2266
                     /locus_tag="VE646_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HZD80009.1"
                     /translation="MPLNLALEGKTYPELSFIVDPDRVAMFRAAVGEGREDVVPPTFV
                     TAAEFEQFPQIIGDPELGLDFTRVVHGEQEYEWFRPLVLGERLTSRSRLAQIRHKAGN
                     GFLTIETELIGTDGEAVVTARSTMIERGGG"
     gene            2259..2696
                     /locus_tag="VE646_08210"
     CDS             2259..2696
                     /locus_tag="VE646_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HZD80010.1"
                     /translation="MAEVGVRLEDLEPGTEIPRRGRVVVREDVKAYADASGDQNPLHQ
                     SEDFARAVGFPGIIAHGMFTMGHLVTSLSDWLGDPEALRRIRVQFRAPVFMGERIVAG
                     GRVRSVDPETRTAMLDVWVTVEREGTTEHPIRKGEAELRFPTG"
     gene            complement(2715..4400)
                     /locus_tag="VE646_08215"
     CDS             complement(2715..4400)
                     /locus_tag="VE646_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80011.1"
                     /translation="MQQPDHPISPEGSDRARRRGLFSRNPPAEDPLDPRLERAVATYQ
                     ELVTERLEQGLQAIRKEASTLMHEVAAEVWRSAGGDRERIQATILESLARDQAIRGLV
                     AHSDERFQDLVVRTARLEDAVGILTEQARAAQRAMTEGVSALEEAAHSPLLGQTAEVR
                     TQLERVARQVAGAFEALAERDRALIETIQHQVREHGELVTRETGHIADSMQAYVQQGV
                     SAMGQLAARVEDQLAAAPAAMEDTVRLLGEQLEMLDERVGIRARDLDGAVHVVEGRFE
                     ERLMGLARLIRSDSEALRQEMVRSAAQHDEVLAEKLDQELGRVSEALTAATRWTVEEM
                     TRRLTDAHGGMIRQQLDEAVSAIDRNMVRMADTLDGGLERLHQRVGHEAAEAADRAIG
                     GRMDSALDRLDAAARTVDRTGADLVKGQHDLEERLGQNLDARIAGLAKMVRSDNRALA
                     DRVQIAAEQEASKQTLRAVKEMQASLPVEVMEIVERRVQDIADQLHRDMRSTAESVAR
                     VGELLERKVDEMASRIGRRYDNDIQVVVERMGDAMHALAELGRPQQHERIELE"
     gene            4621..4694
                     /locus_tag="VE646_08220"
     tRNA            4621..4694
                     /locus_tag="VE646_08220"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4655..4657,aa:Trp,seq:cca)
     gene            4706..5005
                     /gene="secE"
                     /locus_tag="VE646_08225"
     CDS             4706..5005
                     /gene="secE"
                     /locus_tag="VE646_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HZD80012.1"
                     /translation="MNRQMKRAQRRQGGPGVDRRPSPAQGTRRAATQEKRKRTSARQF
                     LKEVRQELKKVNWPTRQELVAYTVVVLVSVTVLTAYVFGLDSIFSRFVVKVFGGG"
     gene            5060..6103
                     /gene="nusG"
                     /locus_tag="VE646_08230"
     CDS             5060..6103
                     /gene="nusG"
                     /locus_tag="VE646_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HZD80013.1"
                     /translation="MSTPETATPATGDADAAIDPVTERSAAGEQEVEATVPPAAPEPT
                     AEDLPLDETLPEGEREEDVVQGSPQDQAPELVISDQQTEDAVEAAVEEEADQEAQAES
                     FTEEAAEEAEAEAAAEEAEAAGEEAEEPEPEPEEPKPEEPVAEAPAEEEPASEPQEEP
                     VETTVADPFKGPGDWYVVHTYAGYENKVKTNLESRIHTMQMEDKIFDVHIPMEDVMEI
                     KSGKKQVVQRKVFPGYLLVKMVYDNDSWYVVRNTPGVTGFVAAGTGSKPTPLSRREVE
                     KILAVKKEEVKPTFRLGYEEGDVVRIISGPFADFNGTISEINVDQSKLKVLVNIFDRE
                     TPVELGFDQVAKV"
     gene            6138..6581
                     /locus_tag="VE646_08235"
     CDS             6138..6581
                     /locus_tag="VE646_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZD80014.1"
                     /translation="MDARRDAPVFAEGEIQIRADVQVVWDLMARIDDWPRWNPDVKSA
                     RLEGPLAEGSRFTWRSGPGTITSTLRSVRSRQEIGWSGRSLGITALHVWRLDSTPGGT
                     VVRTEESWEGLPARLLRGWSHRTVKSAIDSGLRHLKGAAEAPTGS"
     gene            6691..7122
                     /gene="rplK"
                     /locus_tag="VE646_08240"
     CDS             6691..7122
                     /gene="rplK"
                     /locus_tag="VE646_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HZD80015.1"
                     /translation="MPPKKKVLANVKLQIRAGQATPAPPVGTALGQHGVNIMEFCRQY
                     NEQTQSQAGQVIPVELTIYEDRSFSFITKQPPAAELIKQAAGLEKGSGEPNREKVARL
                     TQDQLRRIAEAKMADLNANDVEMAMRIIAGTARSMGVEVAR"
     gene            7119..>7708
                     /gene="rplA"
                     /locus_tag="VE646_08245"
     CDS             7119..>7708
                     /gene="rplA"
                     /locus_tag="VE646_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016477855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HZD80016.1"
                     /translation="MSGNGKRYQTAAKLLEPEREYVPGEAIKLLKEFPPARFDETVEV
                     AFRLGIDPRKADQMVRGTVSLPHGTGRSVRVAVFAAGPKATEAREAGADVVGGEELVD
                     EVMKGNIDFDATVATPDMMAAVGKAGRVLGPRGLMPNPKSGTVTFEIAKAVQEIKAGK
                     LEYKADRTGNLHLVVGKKSFSEQQLVDNYLAVVEEIL"
BASE COUNT         1201 a         2648 c         2805 g         1054 t
ORIGIN      
        1 gggcctgcgc ttcgcgatcc gcgagggcgg ccgcaccgtg ggttccgggc gcgtcaccaa
       61 gatcatcaag tagggacggc tcatggcgaa gaaggaaggc aaccgcccca agatcacgct
      121 cgagtgcacg gagtgccggc ggcggaacta ccacaccgtg aagaaccgga tcaacgaccg
      181 ggaacggctg gagctccgga agtactgccg gtgggagcgc cggtacacgc tccaccgcga
      241 gacccgctag ggctcacccc gcgagggcca gtcctcccgg tcgtcggtca cgtccagcat
      301 ggtgaccgtg ccgtgccgga tggccaggac cagcgcctcg agcctggagt gcaccccgag
      361 cttcgtgatg atgttctggg tgtgcgtgcg aagcgtatgc gggctcatcc ccaggtcctt
      421 ggccatccga tcggagggcc cgcccgaggc catgcgctgc aggatctcca cctcgcgcgg
      481 ggtgaggcgc gccaggcgct gctcggtgct ggcctcccgg tcccggcggt gacgcaggcg
      541 gcgcagcgcg ccctcgatct cctcccggtc gtgcagcggc tcgccccggt ggacccggcg
      601 gaccgatgag gccaccagcg tcaggccctc gctgcggcgc aagagccccg tggcgcccgc
      661 ctgaaccacg cgcgccagca ggtactcgtc ctcgtgcccg gacaggatca ggacgtgagc
      721 gtccggatcg gcgtccttga tccggcgggt ggtctcgatg ccctgcgcgt cgggccgagc
      781 catgtccatc aggatcacgt cggggtcgtg cagccggacc gcctcgatca ccgagggcgc
      841 atcggtgacc acgtccacca cgcgcaggtc cttctcccga ccaagggcca gctgaagggc
      901 ctcgccgaac gtccggtggt cgtccacgat cagcaccttg atgcgcttgt ggtcgttaga
      961 gggcatgggc ggcctcatcg taggggggcg cgaaggccga atcacggaag ctcggcaagt
     1021 tgccgtccgc gcgtgggcgt gagtagcgtg cggccgtggg ccgcttggag ggcaaggtcg
     1081 ccgtgatcac cggagccggt cgcggcatcg gccgggccac cgcgatccgc ttcgccgccg
     1141 agggcgccgc ggtggtggtg aacgacgtcg acgcggatcc ggcccggggg acggcggcca
     1201 cgatccgcga ggcgggcgga cgagccgcgg tctccacgga cgacacggtg gagctcgagg
     1261 gcggtagggc cctcgtcgac ctggccgtga gtgagttcgg tggcctcgac atcctcgtga
     1321 acaacgccgg catcacccgg gaccgcatgt tccacaagct tgacgatgcg cagtgggacc
     1381 tggtgctgga cgtcaacctg cgcacggcgt tccacgccac tctggcagcc tcggcccaca
     1441 tgagggaatc ggccaagcgg gagacggccg agcgggggac gcccctgtac cacaggaagc
     1501 tgaccttcac gacctcgtac tcggccttgg cgggcaacgt cggacaggcc aactacacgg
     1561 cgtccaaggg cgcgatcatc gggctgatgc ggaccctggc ccgcgagctc ggtcccttcc
     1621 gcatcaacgt gaacgcggtg gctcccggct tcatcgagac ccggctcacc gcggccaaga
     1681 gcgagcagga cgagctcggg atccccgagc acctgcgaca gatggccttg atgacgatcg
     1741 cgctcgggcg ctacggacgt cccgaggacg tggccggggt gcacctgttc ctggcctccg
     1801 aggacgcgga ctacgtgacc ggggtggtcc tgccggtcac cggcggccag ctggggagct
     1861 gatcgccgtg cccctgaacc tcgcgctgga gggcaagacc tacccggagc tgtcgttcat
     1921 cgtcgatccg gaccgggtgg cgatgttccg ggccgcggtg ggcgaggggc gcgaggacgt
     1981 ggtcccgccc accttcgtca cggcggccga gttcgagcag ttcccgcaga tcatcggcga
     2041 tcccgagctc ggactggact tcacccgggt ggtccacggg gagcaggagt acgagtggtt
     2101 ccgtcccctg gtcctcggcg agcggctgac cagccggtcc cggctggccc agatccggca
     2161 caaggccggc aacgggttcc tcacgatcga gaccgagctg atcgggaccg acggcgaggc
     2221 cgtcgtgacc gcgcgctcca cgatgatcga gcggggcggt ggctgaggtg ggcgttcggc
     2281 tggaggacct cgagcccgga acggagatcc cgcggcgggg gcgggtggtg gtgcgggagg
     2341 acgtcaaggc ctacgccgac gcctccggcg accagaaccc cctgcaccag agcgaagact
     2401 tcgcgcgcgc cgtggggttc cccggaatca tcgcccacgg catgttcacc atgggccacc
     2461 tggtcacctc gctcagcgac tggctgggcg acccggaggc cctgcgccgg atccgcgtgc
     2521 agttccgggc gccggtgttc atgggtgagc ggatcgtggc gggggggcgg gtccgctccg
     2581 tcgatcccga gacgcggacg gccatgctgg acgtgtgggt caccgtggaa cgcgagggga
     2641 ccaccgagca cccgatccgc aagggcgagg cggagctgcg gttccccacc ggctgaggcg
     2701 gcggggctcg gcgctcactc gagctcgatc cgctcgtgct gctggggacg gcccagctcg
     2761 gccagcgcgt gcatcgcgtc gcccatccgt tccaccacga cctggatgtc gttgtcgtag
     2821 cgccggccga tgcgcgaggc catctcgtcg accttgcgct cgagcagctc gcccacccgg
     2881 gccaccgatt cggccgtgga ccgcatgtcg cgatggagct ggtcggcgat gtcctggacc
     2941 cgccgctcga cgatctccat cacctcgacc ggcaggctgg cctgcatctc cttcaccgcg
     3001 cgcagggtct gcttggacgc ctcctgctcc gccgcgatct gcacccggtc ggccagggcc
     3061 cggttgtccg agcgcaccat cttggccagg ccggcgatcc tcgcgtcgag gttctgcccc
     3121 aggcgctcct ccaggtcgtg ctggcccttc accaggtcgg cgccggtgcg gtccacggtg
     3181 cgagccgcgg cgtccagccg atccagggcc gagtccatgc ggcccccgat cgcgcggtcg
     3241 gcggcctcgg ccgcctcgtg gcccacgcgc tggtgcaggc gctccagccc gccgtccagc
     3301 gtgtcggcca tgcggaccat gttccggtcg atcgccgaga ccgcctcgtc cagctgttgc
     3361 cggatcatgc cgccgtgggc gtcggtcagg cgacgggtca tctcctccac cgtccagcgg
     3421 gtggccgcgg tgagcgcctc ggagacgcgg ccgagctcct ggtccagctt ctcggccagg
     3481 acctcgtcgt gctgcgcggc ggagcgcacc atctcttggc gcagggcctc cgagtccgag
     3541 cggatcaggc gagccaggcc catcagccgc tcctcgaacc gcccctccac cacgtggacc
     3601 gctccgtcca ggtcgcgggc gcggatgccc acgcgctcgt cgagcatctc cagctgctcg
     3661 ccgagcagcc ggaccgtgtc ctccatcgcg gcgggggcgg ccgccagctg atcctccacg
     3721 cgggccgcga gctggcccat ggccgagacg ccctgctgca cgtaggcctg catggagtcg
     3781 gcgatgtgcc cggtctccct ggtgaccagc tcgccgtgct cgcgcacctg gtgctggatc
     3841 gtctcgatca gggcccggtc gcgctcggcc agggcttcga aggcgccggc cacctggcgg
     3901 gccacccgct cgagctgggt gcgcacctcc gccgtctgcc ccagcagggg ggagtgggcc
     3961 gcctcctcca gggcgctcac gccctcggtc atggcccgct gggcggcacg ggcctgctcg
     4021 gtgaggatcc cgaccgcgtc ttccagccgc gcggtgcgca cgaccaggtc ctggaaccgc
     4081 tcgtcgctgt gggcgaccag ccctcggatc gcctgatcgc gtgcgagcga ctcgaggatc
     4141 gtggcctgga tccgctcccg gtccccgccg gccgagcgcc acacctcggc cgccacctcg
     4201 tgcatcaggg tggaggcctc cttgcggatc gcctgcaggc cctgctcgag gcgctcggtc
     4261 accagctcct gataggtggc cacggcgcgc tccaggcgcg ggtcgagggg gtcctcggcc
     4321 ggcgggttcc tggagaagag accgcggcgc ctcgcccggt cggagccctc gggcgagatg
     4381 gggtggtcag gttgttgcat gggcgctcgc attccggggc cggcgcgacg cgtcccgaag
     4441 agtgtagggg agcatgggtg ctgctacacg gaagctgacc agggatctcg ccgctgcccc
     4501 gtgcggagcc ggatcgggcg gagggcatcg tgccggccca cccacctttg ccgcgcccgt
     4561 gcggcacccc ctatactcga tcggtaatgc ccccgatcac cgaccgggcc agcatctcgg
     4621 aggggcgtag ctcaacctgg tagagcaccg gtctccaaaa ccggcggttg ggggttcgag
     4681 tccctccgcc cctgcaagga gcacgatgaa caggcagatg aagcgagcgc agcgccgcca
     4741 gggcgggccg ggcgtggatc gccggccgtc tccggcccag gggacgcgcc gtgccgccac
     4801 gcaggagaag cgcaagcgca cctccgccag gcagttcctg aaggaggtcc gccaggagct
     4861 gaagaaggtc aactggccca cgcgccagga gctggtcgcc tacacggtgg tcgtcctggt
     4921 gtcggtgacc gtgctgacgg cctacgtctt cggcctggac tcgatcttct cccggttcgt
     4981 cgtcaaggtg ttcgggggcg gctaggcacc gatctccacc atgaaccaca tccccgaaga
     5041 ggaacccacg tcagaccaga tgagcacgcc agagaccgcc accccagcca ccggcgacgc
     5101 cgacgccgcg atcgatcccg tcaccgagcg gtccgccgcc ggtgagcagg aggtcgaggc
     5161 cacggtgccc ccggccgcgc ccgagcccac cgcggaggac ctgccgctgg acgagaccct
     5221 ccccgagggc gagcgcgagg aggacgtggt ccaggggtcc ccgcaggacc aggcgcccga
     5281 gctcgtgatc tcggaccagc agaccgagga cgcggtggaa gcggcggtcg aggaggaggc
     5341 cgaccaggag gcccaggccg agtcgttcac cgaggaggcc gccgaagagg ccgaggcgga
     5401 ggcggcagcg gaggaggccg aggcggcagg ggaggaggcc gaggagccgg agcccgagcc
     5461 cgaggagccg aagcccgagg agccggtggc cgaagcgccc gccgaggagg agccggcgtc
     5521 ggagccccag gaggagccgg tcgagaccac ggtcgccgat cccttcaagg gcccaggaga
     5581 ctggtacgtg gtccatacct acgcgggcta cgagaacaag gtgaagacca acctcgagtc
     5641 gcggatccac accatgcaga tggaggacaa gatcttcgac gtccacatcc cgatggaaga
     5701 cgtgatggag atcaagtcgg gcaagaagca ggtggtccag cgcaaggtct tcccgggcta
     5761 cctgctggtg aagatggtct acgacaacga ctcctggtac gtggtccgta acacgcccgg
     5821 cgtgaccggg ttcgtggcgg ccggcacggg ctccaagccc acccccctgt cccgccgcga
     5881 ggtcgagaag atcctggccg tgaagaagga agaggtgaag cccaccttcc gcctgggcta
     5941 cgaggagggc gatgtggtgc gcatcatctc gggacccttc gccgacttca acggcaccat
     6001 cagcgagatc aacgtcgacc agagcaagct gaaggtcctg gtgaacatct tcgaccgcga
     6061 gacgcccgtc gagctcgggt tcgaccaggt cgccaaggtc tagccgcccc ggccggcggc
     6121 gcgggatcgg agggcacgtg gacgccaggc gggacgcccc cgtgttcgcg gagggcgaga
     6181 tccagatccg ggccgatgtc caggtggtgt gggacctgat ggcccgcatc gacgactggc
     6241 cgcgatggaa cccggacgtc aagagcgccc ggctggaggg cccgttggcc gagggatccc
     6301 ggttcacctg gcggtcggga ccgggcacga tcacctccac gctgcggagc gtccggtcgc
     6361 ggcaggagat cggatggagc ggcaggagcc tcgggatcac ggcgctccac gtgtggcgcc
     6421 tggacagcac gcccgggggg acggtggtcc ggaccgagga gtcctgggaa gggctgccgg
     6481 cccggctcct ccggggctgg tcccaccgca cggtgaagag tgcgatcgac tcgggcctgc
     6541 ggcacctgaa gggcgccgcc gaggcgccga ccgggtcgtg agccggtccg gagcggtacc
     6601 ctgagggtcc gcccgcatcc gcgggacggg aacgcgatac gcgggaggcc ggttcgaccc
     6661 ggccgctgga accgcaggag gtgatggggg atgcccccca agaagaaggt cctcgcgaac
     6721 gtcaagctcc agatccgcgc cggtcaggcc acgcccgcgc cgcccgtcgg caccgcgctc
     6781 ggccagcacg gcgtgaacat catggagttc tgccgtcagt acaacgagca gacccagtcg
     6841 caggccgggc aggtcatccc ggtggagctc accatctacg aggaccgctc gttctcgttc
     6901 atcaccaagc agccccccgc cgccgagctg atcaagcagg ccgcggggct ggagaagggt
     6961 tcgggcgagc cgaaccgaga gaaggtggcc aggctcaccc aggaccagct ccggcggatc
     7021 gccgaggcca agatggccga tctgaacgcg aacgacgtgg agatggccat gcggatcatt
     7081 gccggcacgg cacggtccat gggcgtggag gtggctcgat gagcggcaac ggcaagcgct
     7141 accagaccgc ggccaagctc ctggagcccg agcgcgagta cgtgccgggc gaggccatca
     7201 agctgcttaa ggagttcccg cccgcgaggt tcgatgagac ggtcgaggtc gccttccgtc
     7261 tggggatcga cccgcgcaag gccgaccaga tggtccgggg gacggtctcg ctgccccacg
     7321 gcaccgggag gtcggtgcgg gtggccgtgt tcgcggccgg gcccaaggcc accgaggcgc
     7381 gcgaggccgg cgccgacgtg gtgggcgggg aggagctggt cgacgaggtc atgaagggca
     7441 acatcgactt cgatgccacc gtggccacgc cggacatgat ggccgcggtg ggcaaggcgg
     7501 gacgcgtgct cggcccccgg ggcctgatgc cgaaccccaa gagcggcacg gtcaccttcg
     7561 agatcgccaa ggccgtccag gagatcaagg ccggcaagct cgagtacaag gcggaccgga
     7621 ccgggaacct gcacctggtg gtgggcaaga agtccttcag cgagcagcag ctggtggaca
     7681 actacctggc cgtggtggag gagatcct
//
LOCUS       DASQQQ010000217         5571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_73199, whole genome shotgun sequence.
ACCESSION   DASQQQ010000217 DASQQQ010000000
VERSION     DASQQQ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73199"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..2532)
                     /locus_tag="VE646_08250"
     CDS             complement(124..2532)
                     /locus_tag="VE646_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HZD80017.1"
                     /translation="MTQVVTDWSLVFEGTDAEREGFREALCTLGNGYVATRGAAPESS
                     ADGVHYPGTYVAGCYDRLVTQIAGREVGNEDLVNVPNWLPVTFRIGEGPWFGSEAFRT
                     LEVRQELDLRRAVLTRSLRVQDQDGRITAVTDRRFVSMRDAHVLGIEVTLRAQNWSGP
                     ATIRSGLDGGVTNSGVARYRELRGDHLVPLQAEEIGEDTVLLEMETRGSRVHIAQAAR
                     NHILRNGNHQAVSRRTVREGPWIGHDLTVEMTEGAPVTLEKIVTLVTSRDRAVYEPAE
                     DAPIWVARAPGFERLLAEHAVAWAHVWRRCDIRIEGPAEAQGILRLHVFHLLQTVSPN
                     TIGLDVGVPARGLHGEAYRGHVFWDELFIFPFLNQRLSVLTRSLLMYRFRRMPEAKWA
                     AREAGFRGAMFPWQSGTTGREESQTWHLNPRSGRWLPDHSHLQRHIGLAIAYNIWQYY
                     QVTGDLEFVRFYGAPMLIEIARFFDSLASYDRATDRYEIRGVMGPDEYHDAYPDADQP
                     GLNNNAYTNAMAVWLFVRVLEILELFPETQLQELWETLELTREEVDRWDELSRKMRIA
                     FHEDGIISQFEGYGQLQEFDWEGYRRRYGDIQRLDRILESEGDTPNRYKLSKQADVLM
                     LFYLLSADELRELFSRLGYELTPDLIDRNITYYLNRTSHGSTLSRLVHSWVLARSDRE
                     LSWDLFAEALRSDVADVQGGTTAEGIHLGAMAGTVDMVQRGYSGIEVRGDVLWLNPTL
                     PQELSDLELHLHYRGHRVFVSISGKRLVVSTPLSEEAPIKIGLLDEVHEFVPGTTIEV
                     AFSE"
     gene            complement(2529..4097)
                     /gene="otsB"
                     /locus_tag="VE646_08255"
     CDS             complement(2529..4097)
                     /gene="otsB"
                     /locus_tag="VE646_08255"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HZD80018.1"
                     /translation="MSRSMIRLLGRQVRAFVFDMDGVVTDTASVHARSWKRLFDEYLR
                     EHAERSGEPFRTFQDSDYLTYIDGKPRYEGVRDFLASRGIELPQGEPSDPPDRESVCG
                     LGNRKNDYFLAQLREHGADPYPSTVALIRELRQKGIPSAIISSSRNMQEVLQAAGVLG
                     LFDERVDGLVSERLGLRGKPDPAIFLEAARRLGVEAADAVVVEDAISGVEAGRRGGFA
                     VVVGIDRADHADELRQAGADVVVADLADLPIEAGEEPAIGDLPPALDRWDEIAARLRG
                     RTPVVLLDYDGTLTPIVERPEDAVLDPAVRAALRDLARRCAVAVVSGRDLDDVRSMVD
                     VEGIWYAGSHGFDLAGPDGERRQHATEYLPELDRAERDLRSSLADAPGARVERKAFAI
                     AVHYRQATDREVPRISAVVDQAAREHPRLRRTDGKKVFELRPDVPWDKGRASLFLLRT
                     LFPREDGVVPLYVGDDLTDEDAFRVVRGSGIGIVVRGEDDARPTAARYTLRDTGETRA
                     LLEGIGRLVEGPGR"
     gene            complement(4153..5193)
                     /locus_tag="VE646_08260"
     CDS             complement(4153..5193)
                     /locus_tag="VE646_08260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZD80019.1"
                     /translation="MSVRIGIGLFTGQIPSGSDRSFQREYQETLDMVRLAEALGFDSA
                     WVSEHHGASDGYLPSLLVMLGAFAAATERILLGTGVVLTPFHDPIRLAEDAAVVDQLS
                     GGRLIVGLGLGWREEEFRMFGVPVRERLQRTVETVEVLRRAWTGRRFSFQGRSFRYDQ
                     VRVTPPPARPGGPLLFLGGYTERAIRRAGRLGDGYITDAEALTEVQPSMGLFDEGARE
                     AGKDPRAMGLALLQNVFVARAGGPGAWETARPGVTHQLGAYTAWDAGADTPGADQLAP
                     TVDDDETLRASTIAGTPDEVTHGLRPLVTAFGDRDLHLIVRLHYPGMDLETARSAMEL
                     FAEEVLPGLKGD"
     gene            5332..>5571
                     /locus_tag="VE646_08265"
     CDS             5332..>5571
                     /locus_tag="VE646_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZD80020.1"
                     /translation="MEDGRLRPGDRLPTVRELATRLGVAPNTVAKVYRSLGDAAVIVG
                     RGRRGTFVADAGSGAVDPIHALRVEADRYADRAAAL"
BASE COUNT          814 a         2001 c         1913 g          843 t
ORIGIN      
        1 tactcggtga cggtggggct gggttacctg atcccgatcg ccctgatcgt ggctctcgga
       61 tggctcggct accggaggat ccggccccgg ccggtcacct cctcctgacg ggggagccgg
      121 tcctcactcg gagaacgcca cctcgatcgt cgtgcccggc acgaactcgt ggacctcgtc
      181 caggagaccg atcttgatcg gggcctcctc gctgaggggc gtgctcacca ccagccgctt
      241 cccgctgatg gagacgaaga cccggtgtcc tcggtagtgc agatgcagct ccaggtcgga
      301 cagctcctgg gggagggtgg ggttcagcca gagcacgtcg ccgcgcacct cgatgccgct
      361 gtatccccgc tggaccatgt ccacggttcc ggccatggcc cccaggtgga tgccctcggc
      421 cgtcgtgccc ccctgcacgt cggccacgtc gctccgaagc gcctcggcga acagatccca
      481 tgagagctcc cggtccgagc gagccaggac ccaggagtgg accagccgcg acagcgtgga
      541 gccgtgggag gtccggttca ggtagtaggt gatgttgcga tcgatcaggt ccggggtgag
      601 ctcgtacccc aggcgcgaga acagctcccg cagctcatcc gcggagagca ggtagaagag
      661 catgaggacg tcggcctgct tggagagctt gtagcggttg ggggtgtccc cttccgactc
      721 caggatccgg tccagccgct ggatgtcgcc gtaccggcga cggtagccct cccagtcgaa
      781 ctcctggagc tgtccgtacc cctcgaactg gctgatgatc ccatcctcgt ggaacgcgat
      841 ccgcatcttc cggctcagct cgtcccaccg atccacctct tctcgggtga gctccagcgt
      901 ctcccacagc tcctggagct gcgtctcggg gaacagctcg aggatctcga gcacccgcac
      961 gaacagccag acggccatgg cgttcgtgta cgcgttgttg ttcaatcccg gctggtcggc
     1021 atcggggtac gcgtcgtggt actcgtcggg ccccatgacg ccacggatct cgtagcggtc
     1081 ggtggcccgg tcgtagctcg ccaagctgtc gaagaaccgg gcgatctcga tcagcatcgg
     1141 cgccccgtag aagcgcacga actccaggtc gcctgtgacc tggtagtact gccagatgtt
     1201 gtaggcgatg gccagcccga tgtgccgctg cagatgggag tggtccggca gccagcgtcc
     1261 ggagcgcggg ttcaggtgcc aggtctgact ctcctcacgc cccgtggtgc cgctctgcca
     1321 ggggaacatg gcgccccgga agccggcctc gcgcgctgcc cacttcgcct caggcatccg
     1381 gcggaaccgg tacatcagca ggctccgggt gagcaccgac aggcgctggt tcaagaaggg
     1441 gaagatgaac agttcgtccc agaacacgtg gcctcgatac gcctcaccgt gcaggccccg
     1501 ggccgggacc cccacgtcca gcccgatcgt gttcggcgac accgtctgca ggaggtggaa
     1561 gacgtgcagc cgcaggatcc cctgggcctc cgccggcccc tcgatccgga tgtcgcagcg
     1621 tcgccagacg tgggcccacg ccacggcgtg ctcggccagc aggcgctcga agccgggggc
     1681 ccgcgccacc cagatcgggg cgtcctcggc cggctcgtag acggcccgat ccctcgacgt
     1741 gaccagggtg acgatcttct cgagggtcac cggtgccccc tcggtcatct cgacggtgag
     1801 gtcgtggccg atccacggtc cctcccggac cgtccgcctg gagaccgcct gatggttgcc
     1861 gttccggagg atgtggttgc gggcagcctg cgcgatgtgc acgcgtgatc cccgcgtctc
     1921 catctccagc agcacggtgt cctcgccgat ctcctccgcc tgcagcggga cgagatggtc
     1981 gccgcgcagc tcccggtacc gggccacccc ggagttcgtg acaccgccgt ccaggcccga
     2041 gcggatcgtg gccgggccgg accagttctg ggctcggagc gtcacctcga taccgagcac
     2101 gtgcgcgtcc cgcatgctca cgaaccggcg gtcggttacg gcggtgatgc ggccgtcctg
     2161 gtcctggacc cggagggacc gcgtcagcac ggccctgcgg agatcgagct cctgccgaac
     2221 ctccagggtg cggaaggcct cggagccgaa ccagggcccc tcgccgatcc ggaacgtcac
     2281 cggcaaccag ttcggcacgt tgaccagatc ctcgttcccc acctcccggc cggcgatctg
     2341 cgtcaccaac cggtcgtagc agccggccac gtaggtgccc gggtagtgga ccccgtcggc
     2401 gctgctctcg ggcgcggctc cgcgggtcgc gacgtatccg ttgccgagcg tgcacaaggc
     2461 ctctcggaac ccctcccgtt cggcgtccgt gccctcgaag accagcgacc agtccgtgac
     2521 gacctgggtc aacgccccgg accctccacc agacgtccga tgccctccag gagcgcgcgc
     2581 gtctccccgg tgtcgcgcag cgtgtaccgg gcggcggttg gacgggcatc gtcctcaccg
     2641 cggaccacga tcccgatccc cgaccctcgg accacccgga acgcgtcctc gtcggtcaga
     2701 tcgtccccga cgtagagcgg gacgacgccg tcctcacgag ggaacagggt gcggagaagg
     2761 aacagcgatg cccgaccctt gtcccagggc acgtcgggtc ggagctcgaa caccttcttg
     2821 ccgtctgttc gacggaggcg cgggtgctcc ctcgccgcct ggtcgaccac cgccgagatc
     2881 ctggggacct cccgatcggt tgcctgccgg tagtgcacgg cgatcgcgaa ggccttccgc
     2941 tccacccggg cccccggcgc gtccgccagg gaggagcgca ggtctcgctc ggcccggtcg
     3001 agctccggaa ggtactcggt ggcgtgctgt cggcgttcac catcggggcc ggcgaggtcg
     3061 aacccgtggc tgcccgcgta ccagatgccc tccacgtcga ccatcgagcg cacgtcgtcc
     3121 aggtcccgac cgctgaccac cgccaccgcg caccggcggg ccaggtctct cagcgctgca
     3181 cgcaccgccg ggtcgagcac ggcatcctcc ggtcgctcca cgatcggggt gagcgtgccg
     3241 tcgtagtcga ggagcaccac cggcgttcga ccgcgcagcc gagcagcgat ctcgtcccac
     3301 cggtcgagcg ccggaggcag gtcaccgatg gccggctcct ctcccgcctc gatcgggagg
     3361 tcggcgaggt cggcgacgac gacatccgcc ccggcctggc gaagctcgtc cgcgtgatcg
     3421 gcccgatcga tccccaccac caccgcgaag ccgccccggc gacctgcctc cacccccgag
     3481 atggcgtcct ccacgacgac ggcatccgcc gcctcgacgc ccagccgccg ggcggcctcc
     3541 aggaagatgg ccgggtcggg cttgccgcgc aggccgagcc gctcggacac gaggccgtcc
     3601 acccgctcgt cgaagagccc aaggaccccc gcggcctgca gcacctcctg catgttccgg
     3661 ctggaggaga tgatggcgct cgggatgccc ttctggcgca gctcccggat caacgcgacg
     3721 gtggatgggt aggggtcggc gccgtgctcg cggagctggg cgaggaagta gtcgttcttg
     3781 cgattgccca ggccgcacac gctctcgcga tccggggggt ccgatggttc cccctggggg
     3841 agctcgatgc ctcgggaggc gaggaagtcg cggaccccct cgtagcgggg cttgccatcg
     3901 atgtaggtca ggtagtcgga gtcctggaag gtccggaacg gctcaccgga tcgctcggcg
     3961 tgctcgcgca ggtactcgtc gaacagccgc ttccaggatc tggcgtggac ggacgccgtg
     4021 tccgtgacca cgccgtccat gtcgaagacg aacgctcgga cctggcgtcc cagcaaccgg
     4081 atcatcgatc gcgacatggg gcctgtctcc tcggtcgtcc ctcacgatag cgggagcggg
     4141 cggccggagg cgtcagtccc ccttgagacc cgggagcacc tcttcggcga acagctccat
     4201 ggcgctcctc gcggtttcga ggtccatgcc cggatagtgc aggcgcacga tcaggtggag
     4261 gtcgcgatcg ccgaaggcgg tcaccagcgg tcgcaggccg tgggtcacct catcgggcgt
     4321 gccggcgatc gtgctggccc ggagggtctc atcgtcgtcg acggtcgggg ccagctggtc
     4381 ggcaccgggc gtatcggccc ccgcgtccca cgccgtgtag gcgccgagct ggtgggtgac
     4441 gcccggacgg gcggtctccc atgcgcccgg gccgccggcg cgcgcgacga acacgttctg
     4501 caggagggcc aggcccatgg ctcgggggtc tttgcccgcc tcccgagcgc cctcatcgaa
     4561 caggcccatc gacggctgga cctcggtgag cgcctcggca tcggtgatgt agccgtcgcc
     4621 cagccgtccg gccctccgga tcgcccgctc cgtgtatccg ccaaggaaca ggagcggtcc
     4681 ccccggccga gccggcggcg gcgtgacccg gacctggtcg taccggaacg agcgtccctg
     4741 gaacgagaac cggcgaccgg tccaggcccg ccgcaggacc tcgaccgtct ccaccgttcg
     4801 ctggagccgc tcgcggacgg ggacgccgaa catccggaac tcctcctcgc gccaacccag
     4861 cccgagaccc acgatgagtc ggccaccgga gagctggtcc acgaccgcgg cgtcctcggc
     4921 caggcggatc gggtcgtgga acggcgtgag gacgaccccg gttcccagca ggatccgttc
     4981 cgtggccgcg gcgaaggccc ccagcatcac caggagggag ggcaggtagc cgtcggaggc
     5041 gccgtggtgc tcgctgaccc aggcggaatc gaagcccagg gcctcggcca ggcgcaccat
     5101 gtccaaggtc tcctggtact cgcgctggaa gctgcgatcg gatccacttg ggatctgacc
     5161 ggtgaacagc ccgatgccga tccgaacgct catccgcggc aacgctaccc gtcgtgcccg
     5221 ccgggctcaa acgggctccc ggctgcggca tgatgcgctg gtggaggtcc gcatcgatcc
     5281 gtcggcgccg atcccggtct gggtccagct ccgtcggtcg cggcgtcggc ggtggaggac
     5341 ggccggctgc ggcccggcga ccggctgccc accgtccgcg agctcgccac gcggctgggg
     5401 gtggcgccga acaccgtggc caaggtgtat cggtccttgg gggacgccgc cgtgatcgtg
     5461 gggcggggac gacgggggac gttcgtggcg gacgcgggat ccggagcggt ggacccgatc
     5521 cacgccctcc gagtggaggc cgaccggtac gccgatcggg cggccgccct g
//
LOCUS       DASQQQ010000218         4661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_12079, whole genome shotgun sequence.
ACCESSION   DASQQQ010000218 DASQQQ010000000
VERSION     DASQQQ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12079"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..647)
                     /locus_tag="VE646_08270"
     CDS             complement(72..647)
                     /locus_tag="VE646_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZD80021.1"
                     /translation="MTAPWIAAFALLWIAVLLVLFLQLGQFRRAIQVLETAERQLQGS
                     PPHLSQQGLPPGSRIPDIEARDPDGVIVRRADLAGLGGIFLFLSSDCGPCRDLLEDLG
                     RNAGWDGSLPLIAVIDDTPEARLIRFPPGITVLYQTGEAVSRAFDTAVTPHAFLIDAR
                     GTVMHKVIPNTAEQLQELARKAHVLPAGGRA"
     gene            complement(644..1225)
                     /locus_tag="VE646_08275"
     CDS             complement(644..1225)
                     /locus_tag="VE646_08275"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HZD80022.1"
                     /translation="MIEVVSLAARFALAQVFLLAGLSKASAPERFAQTLGTYHVLPER
                     WARPVALWLPRIEVTSGLLLALGLAQALFAGAVFLLLVAFTGAILVNLVRGVEMECGC
                     FGVGRGGRIGPPSIARNIILIAAAIVVAKWPTSVLAIDLPSRVEGSLRTAEGLAVVFS
                     TTIAMTLILLLQEALQLRRTVAAVGERTRGDTS"
     gene            complement(1299..4061)
                     /locus_tag="VE646_08280"
     CDS             complement(1299..4061)
                     /locus_tag="VE646_08280"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZD80023.1"
                     /translation="MTRFLWSQLIHRRSRTLTLGVGILVAAVSFTLLTSAVATSQVRV
                     IGTIEEHFRPAYDILVRPAGSFTELERREQLVQQNYLSGIFGGITFQQYREIRRIPGV
                     EVAAPIANIGYTLPFRSVRIPITRFLSDDERQMYRLRQEWLAHGGSSSYQGGMIYVYY
                     TTGTRFALLPNGLIGEVLRGGRTVPICVAFSRSKGHILSDSPFNLALRQGLYCYSKRT
                     PEISWLPTDEGPLPGGEIGAYADVFFPIPVAAIDPAQEIRLVGLDRAVVAGRILRSDD
                     RVVTEQVAPNAKYRTVPILVSARTYLDETLRVVVERLDLPSEPLFHKAFTSDLHAYRF
                     LNRLQGHDVGKVAVDAVEVYDAYLQRLQGPRPALDYDGYWTSSPVTYRVLGAGRLAAI
                     PNRNAREVYRSRFGGGWAPQENRDTQFRRLTLHRASNLFGPGNVYETPGVRVIGTFDP
                     ELLPGFSPLSEVPLETYYPPAVYPADAPSRRALGGGPLLPTQNLGDYVSQPPLMLTNL
                     RGLRAFTDPTAFEGANRSAPISVIRVRVAGVTGPDEMSRERIRRVAELIHQRTGLAVD
                     ITAGSSPRQMLVELPPGKFGRPALLVREGWVEKGVAIRFLSAVDRKSLALFVLVLVVC
                     AFFLANAAFASVRARRAEIGTLLCLGWGRGTIFRAVLGELALVGLVAGVAGTALAAAL
                     VGLLSLQMSVLRTLLVAPIAVVLAVTAGALPALRASRSTPLDAVRPAVVGRAPRGPVR
                     GVSSMAVVNLRRVRSRALLGAGGLFVAVGALALLLSITLSFRGVLVGTLLGEAISIQV
                     RGVDLASVALAVALGGLSVADVLFLNLRERAPELVTLRASGWEERHLSGLVAAEGLGM
                     GILGAVPGAAAGVLLAWLIGGIDAAVVLAGVLGAGVGILVALAGSAFPALLIGRMTPP
                     AVLAEE"
BASE COUNT          805 a         1644 c         1600 g          612 t
ORIGIN      
        1 ggccgtgatg ggcgccgctg agtcggaacg gtaggtcttg cccacccctt cgagccgaac
       61 ggcgaccccc atcacgcacg acctcccgca gggagcacat gtgccttccg ggcaagctcc
      121 tggagctgct ccgcggtgtt cgggatgacc ttgtgcatga cggtccccct ggcatcgatg
      181 aggaaggcgt ggggcgtcac ggccgtatcg aaggctcgag agacggcctc gcccgtctgg
      241 tacagcaccg tgatcccagg cgggaaccgg atgagccggg cctcaggagt gtcgtcgatc
      301 accgcgatca gcgggaggga gccatcccag ccggcgttcc gtcccagatc ttcgagcaga
      361 tcccggcagg gtccgcagtc cgagctgagg aagaggaaga tgccgcccag tcccgcgagg
      421 tcggcgcggc gcacgatcac gccgtcggga tcgcgggcct cgatatcggg gatacggcta
      481 ccgggtggaa gcccctgctg gctgaggtgc ggcggcgagc cctggagctg gcgctccgcg
      541 gtctcgagga cctggatggc tctccggaac tgtccgagct ggaggaacag gacgagcagc
      601 acggcgatcc agaggagggc gaaggcggcg atccacgggg cggtcacgac gtgtctcctc
      661 tcgtccgttc cccaaccgct gcgacggttc tgcggagctg cagcgcctcc tgcagcagca
      721 agattagcgt catggcgatg gtcgtcgaga agacgacggc caagccctcc gccgttcgaa
      781 gcgaaccctc tactcggctc gggaggtcga tggcaaggac ggacgtcggc cacttggcga
      841 ccacgatcgc cgctgcgatg aggatgatgt tcctcgcgat ggacggcggc ccgatccgac
      901 cgccacggcc gacgccgaag cagccgcact ccatctcgac ccctctgacg aggttcacga
      961 ggatcgcccc cgtgaaggcg acgagcagca ggaacacggc ccctgcgaac agggcctgag
     1021 cgagccctag tgccagcagc aggcccgagg tgacctcgat ccgcggcagc cagagcgcga
     1081 cgggccgggc ccagcgctcg ggcaggacgt gataggtgcc gagggtctgt gcgaagcgct
     1141 cgggggccga cgccttggac agccccgcga ggaggaacac ctgcgcgagg gcgaagcgag
     1201 cagccagcga cacgacctcg atcaccggtt ccacccctcg acggagctgc ctcgttgcga
     1261 ccgcaggaca gacgctgatc ccatcccgcc ctccttcctc actcctcggc cagcaccgcg
     1321 ggcggggtca tccgcccgat cagcagcgcg gggaacgcag agccggccag ggcgaccagg
     1381 atccccacgc cggcgcccag gacgccggcg agcacgacgg cggcatcgat cccgccgatc
     1441 agccaagcga ggagcacccc cgcggcagcc ccgggcaccg ctcccaggat ccccatgccg
     1501 agcccctcgg cggccacgag cccggacagg tgccgctcct cccagccgga ggcccgaagc
     1561 gtcaccagct cgggcgcccg ctcccgcagg ttcaggaaca gcacgtcggc caccgacagc
     1621 cctcccaggg ccacggccag ggccacgctc gccaggtcca ccccccggac ctggatcgag
     1681 atcgcctcgc cgagcagcgt ccccaccagc accccacgga acgacagggt gatcgaaagc
     1741 agcagcgcca gggcccccac cgcaacgaac agcccgccgg cgccgagcag cgcccgcgag
     1801 cgcacccgcc gcaggttcac cacggccatc gaggagaccc cgcggaccgg acctctggga
     1861 gcccgcccga ccaccgccgg gcgcacggca tccagcgggg tggaccggga cgccctcagg
     1921 gcgggcaggg cgcccgcggt cacggccagc accacggcga tcggggccac cagcagcgtg
     1981 cggagcacgg acatctgcag cgacagcaga ccgaccagcg cggccgccag ggcggtgccc
     2041 gcgaccccgg ccaccagccc caccagggcg agctcgccca gcaccgcccg gaagatcgtc
     2101 ccccgccccc accccaggca cagcagcgtg ccgatctcgg cgcgccgtgc ccggaccgag
     2161 gcgaaggcgg cgttggccag gaagaaggcg cagaccacca gcaccaggac gaacagggcc
     2221 aggctctttc ggtccacggc cgacaggaac cggatcgcca cccctttttc cacccagccc
     2281 tcccggacca gcagcgccgg gcgcccgaac ttccccgggg gcagctcgac cagcatctgc
     2341 cggggggagg accccgcggt gatgtccacc gccaggccgg tgcgctggtg gatcagctcg
     2401 gccacccgcc ggatccgctc cctgctcatc tcgtccggcc cggtcacccc cgccacccgg
     2461 acccggatca cgctgatcgg ggccgacctg ttggcgccct cgaaggcggt ggggtcggtg
     2521 aaggcccgga gcccccgcag gttggtgagc atcagcgggg gttgggagac gtagtccccg
     2581 agattctggg tggggagcag cggtccgccc cccagcgccc ggcggctggg ggcatcggcc
     2641 gggtagaccg ccggaggata gtaggtctcc agcggcacct cggacagggg gctgaacccc
     2701 ggaaggagct cggggtcgaa ggtgccgatc acgcggacgc cgggcgtctc gtacacgttg
     2761 ccggggccga agaggttcga ggctcgatga agcgtgagcc ttcggaactg cgtgtcccgg
     2821 ttctcctggg gtgcccaccc tccgccgaag cggcttcggt agacctcgcg ggcgttccgg
     2881 ttggggatgg ctgcaagacg gccggcgccc agcacgcgat acgtcacagg tgaggacgtc
     2941 cagtagccgt cgtagtccag cgccggcctg ggcccctgga gccgctggag gtaggcgtcg
     3001 tagacctcga ccgcatccac cgccaccttc cccacatcgt gcccttgcag ccggttcagg
     3061 aaccggtacg cgtgaaggtc ggacgtgaat gccttgtgga agaggggttc tgatggcagg
     3121 tccaggcgtt ccacgacgac ccgcagtgtc tcgtccaggt acgtgcgtgc gcttaccagg
     3181 atggggacgg tccggtactt ggcgttcggc gccacctgct ccgtgaccac ccggtcatcg
     3241 gaccgcagta tcctcccggc cacgacggcc cgatccagtc cgaccaggcg gatctcctgg
     3301 gccgggtcga tcgcagcgac cgggatgggg aagaagacat cggcgtatgc gccgatctcg
     3361 ccccccggga gcgggccctc gtcggtgggc agccatgaga tctcaggcgt cctcttcgag
     3421 tagcagtaca gcccctgcct cagggccagg ttgaacgggc tatccgaaag gatgtgcccc
     3481 ttggatctgg agaaggccac gcagatcgga acggtgcgac ccccgcgaag cacctccccg
     3541 atgaggccgt tcgggagcag agcgaatcgg gtaccggtcg tgtagtagac gtagatcatg
     3601 cctccctggt aggaagagct cccaccgtgt gcgagccact cttggcggag ccggtacatc
     3661 tgacgttcgt catccgacag gaaacgtgtg atggggatgc gcactgatcg gaaaggaagc
     3721 gtgtagccga tgttggcgat cggcgccgcg acctccacgc cagggattcg cctgatctcc
     3781 cggtactgct ggaaggtgat ccccccgaag atcccgctca ggtagttctg ctggacgagc
     3841 tgctcccgcc gctcgagctc ggtgaacgag cccgccggcc ggaccaggat gtcgtaggcg
     3901 gggcggaagt gctcctcgat cgtgccgatc acgcggacct ggctcgtggc gaccgccgac
     3961 gtgagcaggg tgaagctcac cgcggccacg aggatgccga ccccgagcgt gagggtccga
     4021 ctccgtcggt ggatcagctg gctccacagg aacctcgtca cgactccggt gcaccctccc
     4081 catgaagctc ggtgacgtcc agccggaagg gacgggccac cgcacgcgcg gatgtctact
     4141 caaagcatcg ctgtcagctg cacgggcagc caatcgtgaa gcacgatccg gcgggctcgt
     4201 ggcagacgca gcacgaggtg ctcgaacagt tgtcgaagca ccggtacttg accttggtac
     4261 cggagcacga gatcccgcag gagctatccc tgcagcattg cgacgcaagg gtttcggatg
     4321 ctcgacttgc gccagggagc agggcgagcc caagcccgat cccgacggtg gtcagcgcct
     4381 gccttcccat gcgcaggaac cttcgccggc tgaaggctcc ctgttcggac gtagcctctg
     4441 cgcccattgt cgtgtctccc gcagagatta catggcgaca aggtacaggg tggtgtcccg
     4501 aaacgcaagg gggtcgagcg ctcgcgcggg ggcatccggt tcacctcact cctcggccag
     4561 caccgcgggc ggggtcatcc gcccgatcag cagcgcgggg aacgcagagc cggccagggc
     4621 gaccaggatc cccacgccgg cgcccaggac gccggcgagc a
//
LOCUS       DASQQQ010000219        10992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_212150, whole genome shotgun sequence.
ACCESSION   DASQQQ010000219 DASQQQ010000000
VERSION     DASQQQ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10992
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212150"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VE646_08285"
     CDS             <1..709
                     /locus_tag="VE646_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZD80024.1"
                     /translation="RRLALLGRVNLLAGGEFEALQERHDFLARELDDVRKARRDLLEV
                     IQRIDLEIATTFGSAYRDVAAEFEGLVKDLFPGGEGRLVATEPGDLLNTGIEIEARPG
                     RKRVRRISLLSGGERALVAMAFLFAIFKARPSPFYLMDEVEPALDDVNLHRFLKLIQG
                     FASDAQVLVVTHQKRTMEVAGMMYGVSMRKDGTTKVVAQALDGPERGSAEEAPDQGAA
                     APAVAAGRAGAGRGPLA"
     gene            717..1703
                     /gene="ftsY"
                     /locus_tag="VE646_08290"
     CDS             717..1703
                     /gene="ftsY"
                     /locus_tag="VE646_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017614721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZD80025.1"
                     /translation="MTSAIIITVVAIVLAVVLALVLVRTGRRARPGPEPSPVPRPAPS
                     RREALGSRVRALFGSAAEGEDVWTRLEDLLIKADVGPGTASRIVGDLQERFERGSDPE
                     ALLLEEIAQLLGTDGSLDLPSDRLGVVMMVGVNGSGKTTTIGKLAARLAAQGRRVTVA
                     ASDTFRAAASEQLDVWARRTGAQFVSQQRGADPGAVAFDAVRSASARGADVLIVDTAG
                     RLHTRIPLMDELRKVRRVLEKAAGTPPDEVLLVLDATTGQNGIAQAAAFTAAVDVTGV
                     ALTKLDGTAKGGIVLAVRDQLGVPVKLVGTGEDVGDLEAFDPVAFARRILES"
     gene            1705..2094
                     /locus_tag="VE646_08295"
     CDS             1705..2094
                     /locus_tag="VE646_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZD80026.1"
                     /translation="MAKAEILIVEDHPTMREAMRMVLEHEGFEIREAGDGQAALTMVR
                     ERPPDLLFLDLNIPGASGSDVLRQLKSDPDTRDVRVIVITATGEEGRDYVLSLGADEY
                     FTKPFSPLALLRTVEHVLGPDRAGDEP"
     gene            2249..3595
                     /gene="ffh"
                     /locus_tag="VE646_08300"
     CDS             2249..3595
                     /gene="ffh"
                     /locus_tag="VE646_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HZD80027.1"
                     /translation="MFDTLTDRLNGVFKKLRARGKLHPKQVDNALLDMRTALLEADVA
                     VEVADDLLERVRARALSEEVMTSLTPAQQVIKVVRDELQETMGGTQASFSLPSAKPAV
                     VMMAGVQGSGKTTACAKLARMLREKGKHPLLVAADLQRPAAVDQLFTLGREIGVPVVS
                     DGKDPVKVAKNALKQAGREGLDVMIVDTAGRLHVDPEMMREARRVRDTVRPHHVLMAC
                     DAMTGQDAVVQARAFMQEVDTTGFVLTKLDGDARGGAALSITAATGRPVFFVGTGEKP
                     MDLEPFFPDRMASRIMGMGDVLGLIDKAQENLDQEQAAEAAKKMMEARFTLEDFLVQM
                     REMRKLGPLQDLLAMLPGVPGGKNALKDLAGQVDEGQMARAEAIICSMTREERQNPAI
                     IGGSRRLRIARGSGTTTADVNSLLKEFDATRKMMKQMLGGKGMPKIPGMPNLPGIG"
     gene            3608..3892
                     /gene="rpsP"
                     /locus_tag="VE646_08305"
     CDS             3608..3892
                     /gene="rpsP"
                     /locus_tag="VE646_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HZD80028.1"
                     /translation="MATRIRLRRMGAKKRPFYRVVVADQRSPRDGRFIENIGKYHPLQ
                     EPSLIDIDEERALHWLRVGAQPSRSVQNLLVKSGIWERFQAERKASQAAS"
     gene            3889..4119
                     /locus_tag="VE646_08310"
     CDS             3889..4119
                     /locus_tag="VE646_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HZD80029.1"
                     /translation="MRELLEFIAKELVEDPDAVAVTEVTDDRGLLLQLSVGREDMGRV
                     IGRGGRTARAIRLVMKAAAVRDGTHVAVEILD"
     gene            4121..4633
                     /gene="rimM"
                     /locus_tag="VE646_08315"
     CDS             4121..4633
                     /gene="rimM"
                     /locus_tag="VE646_08315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HZD80030.1"
                     /translation="MEEPAVAVGRITGAHGTRGEVSVLVLSEVEERFADGAELFLEDG
                     RRLTVSSSRPHRGRLLVRFQEVPDRTAAEVLVRGILFVPESMSPPLPEGSWWDHQLVG
                     CRVEVEGGRSLGELRDVIHTPANDVWSVVDAEDAETLIPVLRDVIASVDVKAKAIVVR
                     AVPGLTVPEG"
     gene            complement(4637..5131)
                     /gene="tsaA"
                     /locus_tag="VE646_08320"
     CDS             complement(4637..5131)
                     /gene="tsaA"
                     /locus_tag="VE646_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011821774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HZD80031.1"
                     /translation="MIASTFVRPIGVVHTSADDEVVRAAAPDLPSTIEVFEPFADALE
                     GIDGFSRLIVLTLLDRIDEEARSTLQVKPRGLLRLGLGLDELPTIGVFACDSPVRPNP
                     IAVSIVRLRERVGRSLHVEGLDVFDGTPVLDLKPYGPDRVVPEVTVPAWHEELIRRSG
                     SRRV"
     gene            complement(5151..5597)
                     /locus_tag="VE646_08325"
     CDS             complement(5151..5597)
                     /locus_tag="VE646_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017656172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HZD80032.1"
                     /translation="MRLRMPHRDRSDRSPINIRHFGEGRTLGERVADDVAAWVGSWPF
                     IIIQSTILALWIVVNAVLIRDWIHGKPFDPYPFILLNLVLSFQAAYTGPVVMMSENRQ
                     AAKDRDEAEHDFEVNRRALVLLEELQAGQRDLRSAIADLQRAAGPA"
     gene            5689..6363
                     /gene="trmD"
                     /locus_tag="VE646_08330"
     CDS             5689..6363
                     /gene="trmD"
                     /locus_tag="VE646_08330"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZD80033.1"
                     /translation="MRIDVVTIFPGIFQGPLRESLLGRAIGRGLIDVRVHDLRDFATD
                     RHRQVDDEPYGGGPGMVMKPEPLFAAVGSLGEAAKRVILLSPAGRRLDQALIRELAVE
                     PWLVLLCGRYEGVDERVVEGLPAEELSIGDYVLSGGELPALVVLEAVTRLIPGVIGKE
                     ASHERDSFGPEGLLDHPHYTRPAEFRGLRVPEVLSSGDHAQVQRWRREAALDKTRRNR
                     PDLLEH"
     gene            6402..6740
                     /gene="rplS"
                     /locus_tag="VE646_08335"
                     /pseudo
     CDS             6402..6740
                     /gene="rplS"
                     /locus_tag="VE646_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            6913..7638
                     /gene="lepB"
                     /locus_tag="VE646_08340"
     CDS             6913..7638
                     /gene="lepB"
                     /locus_tag="VE646_08340"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZD80034.1"
                     /translation="MSGRRLGSAEPVGSTAPPTPPDVDARADRRAGRPRDSWVAGGPT
                     PKRHGLPGFFRELPVLVVLAFLLAILLKTFVVQAFYIPSGSMETTLMPGDRVLVEKPF
                     FHPRRGDIIVFQDPDPAPEPDRGLVGGVLHWLSEGVGFTQPPDEDFIKRVIGLPGDVV
                     ELRGGRLFVNGRQVVEAYLHERVDTRSFGPTKVPPGRLFVLGDNRTNSNDSRFGLGMV
                     PLDKVVGKAVVTIWPLSRIGWLH"
     gene            7626..8303
                     /locus_tag="VE646_08345"
     CDS             7626..8303
                     /locus_tag="VE646_08345"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HZD80035.1"
                     /translation="MAPLAIDLGRFERQLRAQGFHAIAGADEAGRGALAGPLVAAAVI
                     LPPDFDLEGLNDSKVLTRRQREACYGRIRERALAVSVRRAMPERIDHRGLHRSNLWLL
                     RRAVVGLPITPDYVLTDGFPISRVPLPCLAMKKGDAVTASVAAASVVAKVSRDGMMDR
                     YHRRFPDYGFDHHRGYGTREHREALARLGPCPIHRRSFRGVGDGTVLVLDDEDRDLID
                     PFPEDAP"
     gene            8300..8620
                     /locus_tag="VE646_08350"
     CDS             8300..8620
                     /locus_tag="VE646_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018776202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 family protein"
                     /protein_id="HZD80036.1"
                     /translation="MNHEDDGIERFEEERELELYREYRDVVPMFAYVVETERRFYLAN
                     RVDVRVRDDGWAEVELHDAWVWDMFRTGRFVTKVRVLTRRDVNVEELRQDLSGPVDSG
                     LTDL"
     gene            complement(8650..9345)
                     /locus_tag="VE646_08355"
     CDS             complement(8650..9345)
                     /locus_tag="VE646_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfocyanin-like copper-binding protein"
                     /protein_id="HZD80037.1"
                     /translation="MWNDTYLGLWLWAVMGLFVLLGVGLMWILARRAKAGVPVVVLAI
                     ALVLAAAVAASAVVTGGPSSHPRGPFIRDRTSCRAPALPGSLVRVTLGDMGSMMGGGG
                     MMGDSTGWSMMGPWPHGSMWIAPRATSIAAADGTVSLVVRNAGSITHELVVLPLAAGR
                     VGQRPVGPDGAVSEDGSLGEASATCAAGEGDGIHPGAEGWVTLPLAPGRYELICNLPG
                     HYAAGMYAELDVR"
     gene            complement(9345..10418)
                     /locus_tag="VE646_08360"
     CDS             complement(9345..10418)
                     /locus_tag="VE646_08360"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HZD80038.1"
                     /translation="MNGLVYSASLVAAFLGGVLALFAPCCIVTLLPNFVGASLRRGLV
                     ALPMTALLFSAGLSVILLPIVLGVGGLGHLVGGMHGPLFVVVGFLLVLLGIYVLSGRR
                     WMLPVPMLRPGRGGNAATSTFLLGVGSGVMSSCCAPVLAGVVAMSALSANPFGALGLG
                     LAYVFGMVFPLLLFALAAARRGQAPGRRTAPRTIRIGGRDVSWTDAASGAMFLLIGLV
                     TFLVGFSGRESITPGVLAAWDRWATARFADLAAFLGRAPVAAQSAILLTLAAAVAIPL
                     VRAVRRAAAPSRQIDPPRDGRGTVLDPVCGMAVDPVRAAASIEWRGHLNHFCAPGCAK
                     AFEEDPDRFAGSSVTRGQASSRP"
     gene            complement(10415..10678)
                     /locus_tag="VE646_08365"
     CDS             complement(10415..10678)
                     /locus_tag="VE646_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80039.1"
                     /translation="MSTGARLSYVTVPHCHFCEDGRAIVRRLSADSGIPAEHLDWHSD
                     AGARLLAIEPAPFPPALYLGDRLLGYGRLSERRLRKLLRRIPA"
     gene            complement(10675..>10992)
                     /locus_tag="VE646_08370"
     CDS             complement(10675..>10992)
                     /locus_tag="VE646_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZD80040.1"
                     /translation="QLQQLQAQLPELQRHGATAVAATMDPVRVSTSVAQQLGLSYPIL
                     EDRDHLLGSAFGTYEVGGHMGSVDQHSVVVLDGRGEVVWRQLAGTTMYVAPDDILAAV
                     ERA"
BASE COUNT         1647 a         3810 c         4029 g         1506 t
ORIGIN      
        1 gcgccgtctg gcgctgctcg ggcgggtcaa cctgctggcg ggcggcgagt tcgaggccct
       61 gcaggaacgt cacgacttcc tggcacgcga gctggacgac gtgaggaagg cccgccgcga
      121 tctgctcgag gtgatccagc ggatcgacct cgagatcgcc acgaccttcg ggtcggcgta
      181 ccgggacgtg gcggcggagt tcgagggcct ggtgaaggac ctgttccccg gcggggaggg
      241 gcgactggtc gccaccgagc cgggcgacct gctgaacacc ggcatcgaga tcgaggcccg
      301 ccccgggcga aagcgcgtgc gccggatctc gctgctgtcc ggtggcgagc gcgcgctggt
      361 ggccatggcc ttcctgttcg ccatcttcaa ggcccgcccc agccccttct acctgatgga
      421 cgaggtggag cccgccctgg acgacgtcaa cctccaccgc ttcctgaagc tcatccaggg
      481 gttcgcgtcg gatgcgcagg tgctcgtggt cacccaccag aagcggacca tggaggtggc
      541 cgggatgatg tacggggtct cgatgcgcaa ggacggcacc acgaaggtgg tggcccaggc
      601 gctcgacggc cccgagcgcg ggtcggcgga ggaggcgccg gaccaggggg ccgcggctcc
      661 ggccgttgcg gcgggccgtg ccggagccgg acgcggtccg ttagcctgaa gggcccgtga
      721 cctccgccat catcatcacc gtcgtcgcga tcgtgctcgc ggtcgtcctc gctctcgtgc
      781 tggtgcggac cggccgtcgc gcccgtcccg gcccggagcc gtccccggtc cccaggccgg
      841 caccgtcgcg acgggaggcc ctcggctctc gggtgcgcgc gctcttcggc tcggccgccg
      901 agggcgagga cgtgtggacc cggctggaag acctcctgat caaggccgac gtcggtcccg
      961 gaaccgcctc ccggatcgtg ggggacctgc aggagcgctt cgagcggggc tccgatcccg
     1021 aggccctgct cctggaggag atcgcccaac tgctgggcac cgacgggtcg ctggacctgc
     1081 cctcggatcg gctcggcgtg gtgatgatgg tgggggtcaa cggttccggc aagaccacga
     1141 cgatcggcaa gctggcggcc cggctcgccg cccagggcag gcgggtgacg gtggccgcca
     1201 gcgacacctt ccgggcggcc gcctccgagc agctggacgt gtgggcccgc cggaccgggg
     1261 cgcagttcgt ctcgcagcag cgtggtgcgg acccgggggc cgtcgccttc gatgccgtcc
     1321 gctccgcatc ggctcggggg gcggacgtgc tcatcgtgga cacggcgggt cggctgcaca
     1381 cgcggatccc cctgatggac gagctccgca aggtccgccg ggtgctggag aaggccgccg
     1441 ggaccccccc ggacgaggtg ctgctggtcc tcgacgccac caccggtcag aacggcatcg
     1501 cgcaggcggc ggccttcacc gcggccgtgg acgtcacggg ggtggccctc acgaagctgg
     1561 acgggaccgc caaagggggt atcgtcctag cggtacgcga ccaactcggt gtgcccgtga
     1621 agctggtggg aacgggagag gacgtgggcg atctggaggc cttcgacccc gtggccttcg
     1681 cccggcggat cctggagagc tgaggtggcc aaggccgaga tcctgatcgt ggaggaccac
     1741 cccacgatgc gcgaggccat gcgcatggtg ctggagcacg aggggttcga gatccgcgag
     1801 gcgggggatg ggcaggcggc cttgaccatg gtccgggagc gtcccccgga cctgctgttc
     1861 ctggacctca acatcccggg ggcgagcgga tcggacgtgc tgcggcagct gaagtccgat
     1921 ccggacacca gggacgtgcg ggtgatcgtg atcaccgcca ccggggagga ggggcgcgac
     1981 tacgtgttgt ccctgggggc cgacgagtac ttcaccaagc cgttcagtcc gctcgccctg
     2041 ctccggacgg tcgagcacgt ccttggaccg gaccgggcgg gggacgaacc ctgaccctcg
     2101 agcggacgcc gacccgcccc cggctccggg cggcgcgatc ccggggcccc gatcgaccgc
     2161 ggaacccttc cgctcggtac aatcgatcga tccggctggt cgttcctgga cccgtcgcac
     2221 cggcgcgggc gtccgcgctc caacggatat gttcgacacc ctgaccgatc gcctgaacgg
     2281 cgtcttcaag aagctccgcg cgcggggcaa gctccacccc aagcaggtgg acaacgccct
     2341 gctggacatg cgcacggccc tgctggaggc cgacgtggcc gtggaggtgg ccgacgacct
     2401 gttggagcgg gtgcgggccc gggcgctctc cgaggaggtc atgacgagcc tgacccccgc
     2461 ccagcaggtg atcaaggtgg tccgggacga gctccaggag accatgggcg ggacccaggc
     2521 ctcgttctcc ctgccctcgg ccaagcccgc cgtggtgatg atggccggcg tgcagggctc
     2581 cgggaagacc accgcctgcg ccaagctcgc tcgcatgctg agggagaagg gcaagcaccc
     2641 gctgctggtg gccgcggacc tgcagcggcc ggcggcggtg gaccagctgt tcaccctggg
     2701 ccgggagatc ggcgtgcccg tggtctccga cggcaaggac ccggtgaagg tggcgaagaa
     2761 cgccctcaaa caggccgggc gtgagggcct ggacgtgatg atcgtggaca cggcggggcg
     2821 cctgcacgtg gacccggaga tgatgcggga ggcgcggcgg gtccgggaca ccgtgcggcc
     2881 gcaccacgtg ctgatggcgt gcgacgcgat gaccgggcaa gacgccgtgg tccaggctcg
     2941 ggcgttcatg caggaggtgg acaccaccgg cttcgtgctc accaagctcg acggggatgc
     3001 gcggggcggg gccgccctgt ccatcaccgc ggcgacgggg cgcccggtct tcttcgtggg
     3061 cacgggcgag aagcccatgg acctcgagcc gttcttcccc gaccgcatgg cgtcgcggat
     3121 catggggatg ggggacgtcc tgggcctgat cgacaaggcg caggagaacc tcgatcagga
     3181 gcaggcggcc gaggccgcca agaagatgat ggaggcccgc ttcaccctgg aggacttcct
     3241 ggtgcagatg cgggagatgc gcaagctcgg tccgctgcag gacctgctgg ccatgctgcc
     3301 gggggtgccc gggggcaaga acgccctgaa ggatctggcc gggcaggtgg acgaggggca
     3361 gatggcccgg gccgaggcca tcatctgctc gatgaccagg gaggagcggc agaaccccgc
     3421 tatcatcggt gggtcccggc gtctgcggat cgcccgcggc tcggggacca cgacggccga
     3481 cgtcaactcg ctgctgaagg agttcgacgc gacccggaag atgatgaagc agatgttggg
     3541 cggcaagggg atgcccaaga tcccgggcat gccgaacctg ccggggatcg gttaggagag
     3601 agaaggcatg gcgaccagga tcaggctgcg acggatgggg gcgaagaagc gccccttcta
     3661 ccgcgtggtg gtggccgatc agcgcagccc gcgcgacggc cggttcatcg agaacatcgg
     3721 caagtaccat ccgctccagg agccctcgct gatcgacatc gacgaggagc gcgcgctgca
     3781 ctggctccgg gtgggcgccc aacccagccg ctccgtgcag aacctcctgg tgaagagcgg
     3841 catctgggag cggttccagg ccgagcggaa ggcatcgcag gccgcctcgt gagggagctc
     3901 ctcgagttca tcgcgaagga gctcgtggag gaccccgacg ccgtcgccgt gaccgaggtc
     3961 accgacgacc gcgggttgct gctccagctg tccgtgggcc gtgaggacat gggccgcgtg
     4021 atcggacgcg gcggtcgcac ggcacgggcc atccggctcg tgatgaaggc cgccgcggtc
     4081 cgcgacggca cgcacgtcgc ggtcgagatc ctcgactgag atggaggagc cggccgtcgc
     4141 ggtcggccgc atcaccgggg cccatggcac ccggggagag gtctcggtcc tggtgctctc
     4201 ggaggtggag gagcgcttcg ccgacggggc cgagctcttc ctggaggacg ggcgtcggct
     4261 caccgtgtcc tcgtcccgcc cgcatcgggg gcggctgctg gtccgattcc aggaggtgcc
     4321 cgaccgcacc gccgccgagg tcctcgtgcg agggatcctg ttcgtccccg agtcgatgtc
     4381 gcccccgctg cccgaaggtt cctggtggga ccaccagctc gtgggatgcc gggtggaggt
     4441 ggagggcggc cggtcgctgg gcgagctccg cgacgtgatc cacacgccgg ccaacgacgt
     4501 gtggagcgtg gtcgatgccg aggatgccga gaccctgatc ccggtgctcc gcgatgtgat
     4561 cgcctcggtg gacgtgaagg ccaaggcgat cgtggtccgg gccgtcccgg gcctcacggt
     4621 cccggagggg tagccctcag acccgccggc tcccgctgcg acggatcagc tcctcgtgcc
     4681 aggccggaac cgtcacctcc ggcaccaccc ggtcggggcc gtagggcttg agatccagga
     4741 ccggggtccc gtcgaacacg tccaggccct ccacgtgcag cgatcgaccc acacgttccc
     4801 ggaggcgcac gatgctcacg gcgatcgggt tcgggcgcac cggcgagtcg caggcgaaca
     4861 ccccgatcgt ggggagctcg tccaggccga ggcccaggcg cagcaggcct cgcggcttca
     4921 cctgcagcgt ggaccgagcc tcctcgtcga tgcgatccag caacgtgagc acgatcagcc
     4981 gggagaaccc gtcgatcccc tccagggcgt cggcgaacgg ctcgaacacc tcgatcgtgg
     5041 agggcaggtc cggcgcggcc gcccgcacca cctcatcgtc cgccgacgtg tgcacgaccc
     5101 cgatcggacg gacgaaggtg gaggcgatca cccggtcagt ctaggggcgc tcaggccgga
     5161 ccggccgcgc gctggaggtc ggcgatcgcg ctccgcaggt cccgctgacc ggcctgcagc
     5221 tcctccagga ggaccagcgc ccgacggttg acctcgaagt cgtgctcggc ctcgtcgcgg
     5281 tccttcgccg cctggcggtt ctcgctcatc atcacgaccg gcccggtgta ggcggcctgg
     5341 aacgacagca ccaggttcag caggatgaac gggtaggggt cgaacggctt gccgtggatc
     5401 cagtcgcgga tcagcaccgc gttcaccacg atccacaggg cgaggatcgt ggactggatg
     5461 atgatgaagg gccacgatcc cacccaggcg gccacgtcgt cggccacccg ctcgcccagg
     5521 gtcctcccct cgccgaagtg acggatgttg atggggctgc ggtcggaacg gtcgcggtgc
     5581 ggcatccgaa ggcgcatcga tccctcctga tccaggtcgt tctccgggct cgagcatcct
     5641 cccgcacgac ggccccgtcg tcgacgacgt cgctgctagc ctgccctggt gcggatcgac
     5701 gtggtgacga tcttccccgg catcttccag ggcccgctcc gcgagagcct gctgggcagg
     5761 gcgatcggcc ggggcctgat cgacgtccgg gtccacgacc tccgcgactt cgccaccgac
     5821 cggcaccgcc aggtggacga cgagccctac ggagggggac ccggcatggt gatgaagccc
     5881 gagccgctgt tcgcggcggt ggggtccctg ggcgaggctg cgaagcgggt gatcctgctc
     5941 tcgccggccg gccgccggct ggaccaggcc ctgatccgcg agctggccgt cgagccgtgg
     6001 ctcgtgctcc tgtgcggccg ctacgagggc gtggacgagc gcgtcgtgga gggcctgccg
     6061 gccgaggagc tgtcgatcgg tgactacgtg ctctccggcg gcgagctccc ggcgctggtg
     6121 gtgctggagg ccgtgacccg gctgatcccc ggcgtgatcg ggaaggaggc atcccacgag
     6181 cgcgactcct tcggccccga ggggttgctc gatcaccccc actacacgcg cccggccgag
     6241 ttccgggggc tccgggtgcc cgaggtcctg tcctccggcg atcacgccca ggtgcagcga
     6301 tggcgtcgcg aggccgccct ggacaagacc cgtcggaacc gccccgatct gctggagcac
     6361 tgaggacgac agcacccacc tgttatcatc cgttccccgc catgaaagcg accgatctcg
     6421 tcgaccagga gtacctgcgc gcggacatcc cgccgttccg tccgggcgac accgtgaagg
     6481 tgcacgtccg ggtggtggag gcggggcggg agcgcgtgca ggtgttccag ggtgtggtga
     6541 tccgtcgcca gggcggaggg ctgcaggaga ccttcaccgt ccgcaagatc tcctttggtg
     6601 tgggggtgga gcggaccttc cccgtccact ccccgtccat ctcgaggctc gaggtggtga
     6661 cgcatggacg ggtccgccgc gcgaagctct actacctgcg cgagcggcgg ggcaagaagg
     6721 cgcggatcag ggagcggcgg atcgagggcg aggagctggc cgccctgcgt cgagccgagg
     6781 ccgatgccgc cgccgcggcc gaggaggccg ccgagcccca ggtggaggag gccgccgagc
     6841 ccgtggtcga cgaggcgtcc accgccgagg gcgaagcgcc cgacgaggtg ggcgccgagg
     6901 aacagggcta gcgtgtcggg gcggcggctc ggctccgcgg agccggtcgg ctcgaccgct
     6961 ccccccaccc ccccggacgt cgacgcccgg gcggatcgtc gggccggtcg gccccgcgac
     7021 tcctgggtgg ccggcggacc cacccccaag cgccacgggc tcccggggtt cttccgcgag
     7081 ctgcccgtgc tggtcgtcct ggcgttcctg ctggccatcc tgctcaagac cttcgtggtc
     7141 caggccttct acatcccctc ggggtcgatg gagaccaccc tgatgcccgg ggaccgtgtg
     7201 ctggtggaga agcccttctt ccatccacga cgcggggaca tcatcgtgtt ccaggacccc
     7261 gaccccgccc cggagcccga tcgcggactc gtcggaggcg tgctccactg gctctcggag
     7321 ggcgtggggt tcacccagcc gcccgacgag gatttcatca agcgcgtgat cgggctgccc
     7381 ggcgacgtcg tggagctccg cggcggccgg ttgttcgtca acggccgaca ggtggtcgag
     7441 gcctacctgc acgagcgggt ggacacccgc tccttcggac cgaccaaggt gcccccggga
     7501 cggctgttcg tgctcggcga caaccgcacc aactcgaacg actcccggtt cggcctgggc
     7561 atggtgcccc tcgataaggt ggtggggaag gcggtggtga cgatctggcc gctctcccgg
     7621 atcggatggc tccactagcg atcgacctcg ggcggttcga gcgccagctc cgggcgcagg
     7681 ggttccacgc catcgccggc gccgacgaag cgggccgggg agcgctggcc ggcccgttgg
     7741 tggcggcggc cgtgatcctg ccgccggact tcgatctgga gggtctcaac gactccaagg
     7801 tgctcacccg tcggcaacgc gaggcctgct acgggcggat ccgggagcgg gccctggccg
     7861 tctcggtcag gcgcgcgatg cccgagcgca tcgaccaccg gggcctgcat cgatcgaacc
     7921 tgtggctgct ccgccgcgcc gtggtggggc tgccgatcac gcccgactac gtgctcaccg
     7981 acgggttccc catctcccgt gtcccgctcc cctgcctggc catgaagaag ggggatgcgg
     8041 tgacggcctc ggtggcggcc gcctccgtgg tggccaaggt gagccgggat ggcatgatgg
     8101 accggtacca ccgccgcttc cccgactacg ggttcgacca ccaccgtggg tacggcaccc
     8161 gggagcaccg cgaggccctg gcccgcttgg gcccctgtcc gatccatcgc cgttccttcc
     8221 ggggggtggg ggacggcacc gtgctggtgc tggacgacga ggaccgcgac ctgatcgatc
     8281 cgttccccga ggatgccccg tgaaccacga ggacgacggc atcgagcggt tcgaggagga
     8341 gcgggagctc gagctgtacc gcgagtatcg cgacgtggtc ccgatgttcg cctacgtggt
     8401 ggagaccgaa cgccgcttct acctggcgaa ccgggtcgac gttcgggtcc gagacgatgg
     8461 gtgggcggag gtggagctgc acgacgcttg ggtgtgggac atgttccgga ccggccggtt
     8521 cgtgaccaag gtccgtgtgc tcacccggcg cgacgtgaac gtggaggagc tccgccagga
     8581 cctctccggt cccgtcgaca gcgggctgac cgatctctag gggccgagcc ctccgagcca
     8641 cggccgcggc tatcggacgt cgagctccgc gtacatgccg gccgcgtagt ggcccgggag
     8701 gttgcagatc agctcgtacc ggcccggggc caggggaagg gtgacccagc cttcggcccc
     8761 cggatggatc ccgtcgcctt cgccggcggc gcaggtggcc gaggcctcgc ccaggctccc
     8821 atcctcgctc accgcaccgt ccggccccac cggacgctgg cccacccttc ctgcggcgag
     8881 cggcaggacg accagctcgt gggtgatgct gccggcgttg cggacgacca gggacaccgt
     8941 tccgtcggca gcggcgatgc tggtggcacg gggggcgatc cacatcgatc cgtggggcca
     9001 cggccccatc atcgaccacc ccgtgctgtc gcccatcatc ccgcctccgc ccatcatcga
     9061 gcccatgtcc cccagggtca cccggaccag ggaaccgggg agggccggtg cccggcacga
     9121 ggtccggtcg cggatgaagg gacctcgggg gtggctcgaa ggtcctccgg tcaccacggc
     9181 agaggcggcc acggccgccg ccagcaccag cgcgatcgcc agaacgacca ccgggactcc
     9241 ggccttggct ctgcgggcga ggatccacat gaggccgacg ccgagcagca cgaacagccc
     9301 catcaccgcc cagagccaca gaccgaggta ggtgtcgttc cacatcacgg ccgtgagctg
     9361 gcctggccgc gcgtcaccga agatccggcg aaccggtccg ggtcctcctc gaacgccttc
     9421 gcgcagccgg gggcacagaa gtggttgagg tggcccctcc actcgatgct cgcggcggct
     9481 cgcaccggat ccacggccat gccacacacg ggatcgagga ccgtcccccg cccgtcccgc
     9541 ggcgggtcga tctgtctcga cggcgctgcc gcccttcgaa cggcgcgcac cagggggatc
     9601 gccaccgcgg ccgccagcgt caggaggatc gccgactggg cggccaccgg ggcccggccg
     9661 aggaacgcgg ccagatccgc gaagcgtgcg gtggcccatc ggtcccacgc cgccaggacc
     9721 ccgggggtga tgctctcccg cccgctgaac ccgaccagga acgtgacgag cccgatcagc
     9781 aggaacatcg cgccggatgc ggcatccgtc cacgacacgt ccctgccgcc gatccggatc
     9841 gtgcgggggg ctgtcctcct ccctggggcc tggccccggc gggccgcggc cagtgcgaac
     9901 aacaagagcg ggaagaccat gccgaacacg tacgcgagcc cgagaccgag ggcgccgaac
     9961 gggttcgcgc tgagggcgga catcgccacc accccggcca agaccggcgc gcagcaggag
    10021 ctcatgacgc ccgaccccac ccccaggagg aaggtggacg tggccgcgtt gccgccgcga
    10081 cccggacgca gcatcggcac cggcagcatc cagcgacggc ccgacaggac gtagatcccg
    10141 agcaggacca gcaggaaccc caccaccacg aacagggggc cgtgcatgcc ccccaccagg
    10201 tggcccagac cgcccacccc cagcacgatg ggcagcagga tcaccgacag gccggcgctg
    10261 aacagcagcg cggtcatggg caaggccacc aggccccggc gaagtgacgc gcccacgaag
    10321 ttcgggagca gggtcacgat gcagcagggg gcgaacaggg cgagcacgcc ccccaggaac
    10381 gcggccacca gcgaagccga atagaccagg ccgttcatgc cgggatccgc cgcagcagct
    10441 tgcggaggcg acgctcggag aggcgaccgt agccgagcag ccggtccccg aggtacaggg
    10501 cgggagggaa cggggccggc tcgatcgcga gcaggcgggc gccggcgtcg ctgtgccagt
    10561 cgaggtgctc ggccgggatg ccgctgtcgg ccgacagccg ccgcacgatg gccctgccgt
    10621 cctcgcagaa gtggcagtgg ggcaccgtga cgtaggagag ccgggcgccg gtgctcatgc
    10681 ccgttccacc gcggccagga tgtcatcggg ggcgacgtac atcgtggtcc cggcgagctg
    10741 tcgccacacc acctcgcccc gcccgtccag caccaccacg gagtgctggt cgaccgagcc
    10801 catgtggcca ccgacctcgt aggtcccgaa cgccgagccc agcaggtggt cccggtcctc
    10861 gaggatggga taggagagcc ccagctgctg tgcaaccgag gtcgagaccc ggaccggatc
    10921 catcgttgcc gccacggcgg tcgccccgtg gcgctggagc tcggggagct gtgcctggag
    10981 ctgctggagc tg
//
LOCUS       DASQQQ010000220         3646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_212181, whole genome shotgun sequence.
ACCESSION   DASQQQ010000220 DASQQQ010000000
VERSION     DASQQQ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212181"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VE646_08375"
     CDS             complement(<1..504)
                     /locus_tag="VE646_08375"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZD80041.1"
                     /translation="MAHEVRTEEGEVQIGTEACFQHLLEALEDPDPKIRRLAVCGLAA
                     CGDLRCVEPVVRTLHDPDAEVRGRAALALGRMGHPQAAPALVEAATGSDEPLRRAALL
                     ALGELDAGLEVLADVLRRGRPADGARRPGPPAGPPGAPPAVPGSGTLLGGARTPSSRG
                     RCPRPLPR"
     gene            629..1633
                     /locus_tag="VE646_08380"
     CDS             629..1633
                     /locus_tag="VE646_08380"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HZD80042.1"
                     /translation="MPPPEIPLLRVGGSHRDAGRRIGRACAETIRKAVRFDDQVPCGR
                     TRQEQLGLAREYLRVTAEAYPWYVDELEGAAEAAEVDPLAVFACAVEEIWYEPRRPAL
                     QGRCTDLVAVPPATEGDRTLVAHNNDLSRSYQEDLVAIEWSIDGDPTVLTLGNGIWIS
                     AGWSSAGISFTGNELAPNDERIGIPREVQFRAMLRRSSLDAAVEEALREDRASSYNQV
                     LVDRSGRAVDVEGSATHAELTGPDERGHLVHTNHYVCDAMRRYEGDPGYAVRSSFRYR
                     RAAELIAARPPGTVTADTMRGILADHRNPPSRSAATPSSTAVRPRPSSGAWPTSPRDA
                     "
     gene            complement(1701..2888)
                     /locus_tag="VE646_08385"
     CDS             complement(1701..2888)
                     /locus_tag="VE646_08385"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZD80043.1"
                     /translation="MATYFANTTLFDGRRLATKAGVLTSNGTIAWAGAHARAPREALA
                     AHEVDASGKTLSPGLIDCHVHLCFDGSADFAGEAREMTSDTVAALRVIRNAAANLDRG
                     VTTVRDLGGRGAVVCEVARAVDRGRIPGPRILAAARALTITGGHGHSIGIAVEVDGPD
                     AVRAAVRRQIRDGATAIKIIATGGVLTPGITFDFTAFTPDELGAAVDEAHKWDRVIAA
                     HAIGPAGIVDAVRAGVDSIEHGSMLTPEGARLMKDRGTVYVPTISAIRGMVEHPDEVP
                     AYAVEKATALAGSARDAFRRALRAGVAIACGTDAGTPFNPHGNAPHEIVSMVEWGMTP
                     ARAMQAATSSAAELLRLQRVGVIEEGRAADLVLYDGNPMDRIEVVTKPAMVMMAGEVV
                     AGP"
     gene            complement(2932..3408)
                     /locus_tag="VE646_08390"
     CDS             complement(2932..3408)
                     /locus_tag="VE646_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80044.1"
                     /translation="MAADTLFWFRFWLFWHIAGVLGFLAAHGATAAVAFRLRSERDRG
                     RIRALLELSRSTRSWMYVSLIVLIGAGVANGFVLHVWGQGWIWAAIGLLAVLLLAAFP
                     MAVPYYIRVRRAVTGEPAPSPDELEVLLRAPRPIVIAVVETAGILVILWLMVFRPF"
     gene            complement(3423..>3646)
                     /locus_tag="VE646_08395"
     CDS             complement(3423..>3646)
                     /locus_tag="VE646_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZD80045.1"
                     /translation="EQEAGLIRDLYLSGRRREAAASVPDALVDEVALVGSIARIRDRL
                     AVWREAGVTTLLVQTRDRVCLSAVVEAAR"
BASE COUNT          524 a         1391 c         1270 g          461 t
ORIGIN      
        1 tcgtgggagc ggccgtgggc atcgccctcg gcttgacggt gttcgcgctc cgccgaggag
       61 cgtccccgag ccagggacgg ctggaggcgc gcctggaggc ccggctggag gtccaggccg
      121 ccgagctccg tcggctggcc gtcctcggcg caggacgtcg gccagcacct ccagccccgc
      181 gtccagctct cccagggcca ggagcgcggc gcgacgcagc ggctcgtcgg atccggtcgc
      241 ggcctccacc agggccggcg cggcctgggg atgacccatc cgtcccagcg cgagcgccgc
      301 ccggccccgg acctcggcgt cgggatcgtg gagcgtcctc accaccggct ccacgcagcg
      361 gaggtccccg cacgcggcca ggccgcacac ggccagacgg cggatcttgg ggtcgggatc
      421 ctccagcgcc tcgagcaggt gctggaagca ggcctcggtg ccgatctgca cctcgccttc
      481 ctcggtcctc acctcgtgcg ccatccccat cacccccgct cgggttccgc attcatgaac
      541 ccaggtctgg gcccacccgc catggggcga gggaccccag gcgggtaggt cgcacggctc
      601 ggtggccgga gccgctacga tgccccagat gccgccaccc gagatcccgc tgctgcgggt
      661 cggcgggtcc catcgggacg ccggccgacg gatcgggcgc gcgtgcgcgg agacgatccg
      721 gaaggccgtc cggttcgacg accaggtccc ctgcggacgc acgcgccagg agcagctcgg
      781 cctcgcccgc gagtacctgc gggtgacggc ggaggcctac ccctggtacg tggacgagct
      841 ggagggagcg gcggaggcgg ccgaggtcga tccgctggcg gtgttcgcgt gcgcggtgga
      901 ggagatctgg tacgaaccgc gtcggccggc cctgcagggc cggtgcaccg acctggtggc
      961 cgtgccgccg gccaccgaag gcgaccggac cctggtggcc cacaacaacg acctctcacg
     1021 ctcgtaccag gaggatctcg tggccatcga gtggtcgatc gacggcgatc cgacggtcct
     1081 caccctgggc aacggcatct ggatcagcgc cgggtggagc tcggccggca tctcgttcac
     1141 cgggaacgag ctggcgccga acgacgagcg gatcggcatc ccccgggagg tccagttccg
     1201 ggccatgctc cgccggagct cgctggatgc cgcggtggag gaggcgctgc gtgaggaccg
     1261 agcctcgtca tacaaccagg tgctggtgga tcgctccggc cgcgcggtcg acgtggaggg
     1321 ctcggccacg cacgccgagc tcaccggccc ggatgagcga ggccacctgg tccacacgaa
     1381 ccactacgtc tgcgatgcga tgcgccggta cgagggcgac cccggctacg ccgtgcgctc
     1441 ctcgttccgc taccggcggg cggcggagct gatcgccgca cggccaccgg gtaccgtcac
     1501 cgcggacacg atgcgcggga tcctcgccga ccaccggaac cccccgagtc gatctgccgc
     1561 caccccgagc tccacggcgg tgagaccgcg accgtcttct ggagcgtggc cgacgtcacc
     1621 gagggacgcg tgaccttcgg ccgcggcaac ccctgccact ccacggcgca gacccacacc
     1681 ttcgcctgac cccggcccgg tcagggaccg gccaccacct cgcccgccat catcaccatg
     1741 gccggcttgg tcaccacctc gatccgatcc atcgggttgc cgtcgtacag gaccaggtcg
     1801 gcggcgcgac cctcctcgat cacgccgacg cgctgcaggc ggagcagctc ggcggcgctc
     1861 gaggtggccg cctgcatcgc ccgggccggg gtcatgcccc attccaccat gctcacgatc
     1921 tcgtgcggcg cgttgccgtg cgggttgaac ggtgtgccgg catcggtccc gcaggcgatc
     1981 gccaccccgg cccgaagggc gcgacggaag gcatcgcggg cgctgccggc cagcgcggtg
     2041 gccttctcca cggcgtacgc ggggacctcg tctggatgct ccaccatgcc gcggatcgcc
     2101 gagatggtgg ggacgtagac ggtcccgcgg tccttcatca gccgggcccc ttcgggggtc
     2161 agcatcgatc cgtgctcgat cgagtccacg ccggcccgaa ccgcgtccac gatcccggcc
     2221 ggaccgatcg cgtgggccgc gatcacccgg tcccacttgt gggcctcgtc cacggccgcc
     2281 ccgagctcgt cgggggtgaa cgccgtgaaa tcgaaggtga tcccgggggt caggacgccc
     2341 ccggtggcga tgatcttgat cgccgtcgcc ccgtcccgga tctgccggcg caccgccgcc
     2401 cgcacggcgt ccggaccgtc cacctccacg gcgatcccga tgctgtggcc gtgacctccg
     2461 gtgatggtga gggcgcgggc cgcggccagg atccggggcc cggggatccg gccccggtcc
     2521 acggcccggg ccacctcgca gacgacggcg ccccgccccc ccagatcccg caccgtggtc
     2581 acgccgcgat cgagattggc tgcggcgttg cggatcaccc gcagcgcggc caccgtgtcg
     2641 ctggtcatct cgcgggcctc gccggcgaag tcggccgagc catcgaagca caggtggacg
     2701 tggcaatcga tcagcccggg cgagagggtc ttgcccgacg cgtcgacctc gtgggccgcc
     2761 agcgcctccc ggggggcccg ggcgtgcgcg ccggcccagg cgatcgtgcc gttggaggtg
     2821 aggacgccgg ccttggtcgc caagcgtcgg ccgtcgaaga gcgtggtgtt cgcgaagtac
     2881 gtggccatcc ggtcctcctc ggccgcatca tcgcagaagc tcgcccggag ctcagaaggg
     2941 gcggaagacc atgagccaga ggatcaccag gatccccgcc gtctccacga ccgcgatcac
     3001 gatcggccgg ggagcacgca ggagcacctc cagctcgtcg ggggacggcg ccggctcgcc
     3061 cgtcaccgcc cgacggaccc ggatgtagta cggcacggcc atggggaagg ccgccagcag
     3121 gaggacggcc aggagcccga tcgccgccca gatccatccc tggccccaca cgtgcaggac
     3181 gaagccgttc gccacccccg cgccgatcaa cacgatcagc gagacgtaca tccagctccg
     3241 ggtgctcctg gagagctcga gcagcgcgcg gatccgcccc cggtcccgtt ccgagcgcag
     3301 acggaaggcc accgcggccg tggccccgtg cgccgccagg aagccgagca cgcccgcgat
     3361 gtgccagaac agccagaacc ggaaccagaa cagcgtgtcc gccgccacca ccccctcctc
     3421 cgctatcgag ccgcctccac gaccgcggac aggcagaccc ggtcccgcgt ctggaccagc
     3481 agggtggtga cccccgcctc ccgccacacg gccaggcgat cccggatccg ggctatcgac
     3541 cccaccagcg ccacctcgtc caccaacgcg tcgggaacgg aggccgccgc ctcgcgccgc
     3601 cgaccggaca ggtacaggtc gcggatcagg ccggcctcct gctcgt
//
LOCUS       DASQQQ010000221         4296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_212222, whole genome shotgun sequence.
ACCESSION   DASQQQ010000221 DASQQQ010000000
VERSION     DASQQQ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212222"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VE646_08400"
     CDS             complement(<1..261)
                     /locus_tag="VE646_08400"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600530.1"
                     /note="catalyzes the phosphorylation of
                     N-acetyl-L-glutamate to form N-acetyl-L-glutamate
                     5-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HZD80046.1"
                     /translation="MSRPQLPPQIRERTVAKARTLLEALPFIQEHWGTVIVVKLGGAA
                     MDRAPLSRSFAEDLALLQHVGIRPLVVHGGGPQVTEVSRRLGL"
     gene            complement(258..1451)
                     /gene="argJ"
                     /locus_tag="VE646_08405"
     CDS             complement(258..1451)
                     /gene="argJ"
                     /locus_tag="VE646_08405"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HZD80047.1"
                     /translation="MSVTFPRGFRAAGVTAGMKASGRPDLALLVGDRDCSAAGLFTTN
                     AFAAAPVRLSRARLASGTARAVLVNSGQANAATGPRGERDALEATAAAADLLGIAPGS
                     VLACSTGVIGESLHMEQLLTALPPLVAAASAQGGDAFAEGILTTDTKAKQAIAETATH
                     RVGGAAKGVGMIAPDLQLATMLAFVTTDASVPPSHLGRLAAEALEPGFEALTVDGCTS
                     TNDTVLLLSSGAAGGDPVVPGTAAWGSLAAACGEVGASLVEQLAHDAEGANHVLLIDV
                     TGALDTEEASAAARAVADSLLVKTAVFGGDPNPGRILQAVGSSGASVIPGSVDAWIGD
                     VQVIAAGTIPADMRGAGGERVRRALKERDVRVRVRLGDGPGASRALGVDLSYEYVRIN
                     AEYTT"
     gene            complement(1448..2509)
                     /gene="argC"
                     /locus_tag="VE646_08410"
     CDS             complement(1448..2509)
                     /gene="argC"
                     /locus_tag="VE646_08410"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HZD80048.1"
                     /translation="MPSLAPFRVAVLGASGVTGGELIRLLLEHPSASVSFVGAETSAG
                     RRLAEVHPHLAASPLGDEVLGPMEPAAVAGIADAAFLALPHGASSRLAPPLVEFGLRV
                     IDLSGDFRLPAGSYPDWYGFIHPAPAWLDKAVYGLPELFATRIRDAALVANPGCFPTP
                     VVLGLAPLLRAGAIGEGAIHVDGKTGLSGAGGRVAPAMQHLAAEESVRPYRFPRHQHT
                     PEMEHALALTGAEDVRISFVPHLVPAVRGVLVTCFARLADPDTSSERLTAILDRAYEE
                     APFVRVLPPGAMVDTKRTRGANLVELQAAADPRTGTAIVVGAIDNLVKGAAGQAVQNL
                     NLMHGLNPGTGLPAVGMYP"
     gene            complement(2596..2931)
                     /locus_tag="VE646_08415"
     CDS             complement(2596..2931)
                     /locus_tag="VE646_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80049.1"
                     /translation="MDFADALRDRGFAPSTERAPRGAAVYTAHPNRYTTYTVQAFEDG
                     TALFTFEFAIGAYLATKGIQIGSDETLNQFAYPRQDIRGRQERTWLTMAVEQAEALLA
                     DVRLDRPEG"
     gene            complement(2976..3281)
                     /locus_tag="VE646_08420"
     CDS             complement(2976..3281)
                     /locus_tag="VE646_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014441073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HZD80050.1"
                     /translation="MIGRMWRGWTAPERAEGYVGYLEESGVAALRSTPGNQGVLVLHR
                     TDGNREEFLVLSLWESLESVKAFAGEDVTVARFYPRDDEFLVDRELTCSHYEVPIGP"
     gene            complement(3317..>4296)
                     /gene="rmuC"
                     /locus_tag="VE646_08425"
     CDS             complement(3317..>4296)
                     /gene="rmuC"
                     /locus_tag="VE646_08425"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HZD80051.1"
                     /translation="VGAAVGIALGLTVFALRRGASPSQGRLEARLEARLEVQAAELRR
                     LADASSARDRQAEHLGDRLDGARRVLEELAVRERERRDQESEAWGVIRRLSTVLAGGS
                     GRGRAGENVLRQHLAELPPDMLETDFRVNGKVVEFALRLPDGRRLPVDSKWPAVAELE
                     ALESAESAEREARGREVERVVALRAREVAQYLDPALTSPVAVAAVPDAAYAVLRKAHA
                     DAFARGVVIVPYSTALPVLLFLYSLVNHFADPGDVRSALARIAALLDAMEAVVENRFA
                     RAAAMIQNGTDELRSQLSKARGSVARAGAGGSLAPGEPPAAGERLRALR"
BASE COUNT          599 a         1642 c         1514 g          541 t
ORIGIN      
        1 gagtcccagg cgcctcgaga cctccgtcac ctgggggcct ccgccgtgca ccaccagggg
       61 gcggatcccc acgtgctgga ggagggccag atcctcggcg aaggagcgcg agagcggtgc
      121 ccggtccatg gccgccccgc ccagcttgac cacgatcacg gttccccagt gctcctggat
      181 gaagggcagg gcctcgagga gcgtccgggc cttggccacc gtgcgctcgc ggatctgggg
      241 cggcagctgg ggacggctca cgtcgtgtac tccgcgttga tccgcacgta ctcgtaggag
      301 aggtcgacgc cgagcgcccg gctggcaccc ggcccgtcgc ccaggcggac cctcacccgc
      361 acgtcccgct ccttcagggc gcggcggacc cgctcgccgc ccgcaccacg catgtccgcc
      421 gggatcgtcc cggccgcgat cacctggacg tccccgatcc acgcgtccac cgatcccggg
      481 atgacggacg ctccggacga gcccaccgcc tgcaggatcc ggccggggtt gggatcgccc
      541 ccgaagacgg cggtcttcac cagcagcgag tcggccacgg ccctggccgc cgcgctcgcc
      601 tcctcggtgt cgagcgcacc ggtgacgtcg atcagcagca cgtggttcgc accctccgcg
      661 tcgtgggcca gctgctcgac caacgacgcg cccacctcgc cgcaggcggc ggccagcgat
      721 ccccaggccg ccgtgccggg cacgaccgga tcgccacccg ccgccccgct ggaaagcagc
      781 aggacggtgt cgttcgtgct ggtgcagccg tcgaccgtga gcgcctcgaa cccgggctcg
      841 agcgcctcgg cggccagccg gccgaggtgc gaagggggca ccgacgcgtc cgtggtgacg
      901 aacgccagca tcgtggcgag ctgcaggtcg ggcgcgatca tgcccacgcc cttcgcggcg
      961 cctcccaccc ggtgggtggc cgtctcggcg atcgcctgct tggccttcgt gtccgtggtg
     1021 aggatgccct cggcgaacgc gtcgcccccc tgggccgacg ccgcggccac caggggcggc
     1081 agggcggtca ggagctgctc catgtgcagc gactcgccga tcacgcccgt ggagcaggcg
     1141 agcacggacc cgggcgcgat cccgagcagg tcggccgccg ccgcggtggc ctccagagcg
     1201 tcgcgttccc cccgcggccc ggtggccgcg ttggcttggc cgctgttcac cagcaccgcc
     1261 cgagcggtcc ccgaggcgag ccgggcgcgg gacaggcgaa cgggagcggc ggcgaacgcg
     1321 ttcgtcgtga acaaacccgc ggccgagcag tcccggtcgc ccaccagcag cgcgaggtcc
     1381 gggcgcccgc tggccttcat gccggcggtg acgccggccg cccggaagcc gcgggggaag
     1441 gtcacgctca cgggtacatc cccacggcgg gcagcccggt gcccgggttc agcccgtgca
     1501 tcaggttgag gttctggacg gcctgccccg ctgcgccctt caccaggttg tcgatcgccc
     1561 ccacgacgat cgccgtgccg gtcctggggt ccgcggccgc ctggagctcc accaggttgg
     1621 ccccgcgggt ccgcttcgtg tccaccatcg cgccgggagg cagcacgcgg acgaacggcg
     1681 cttcctcgta ggcccggtcc aggatcgccg tgaggcgctc ggagctcgtg tccgggtcgg
     1741 cgagccgcgc gaagcaggtc accaggaccc ctcggacggc cggcaccaga tgcgggacga
     1801 acgagatccg cacgtcctcc gccccggtca gtgcgagcgc gtgctccatc tctggcgtgt
     1861 gctggtgccg cgggaagcgg tagggacgca cgctctcctc ggcggccagg tgttgcatgg
     1921 ctggggccac ccgacccccc gcgccggaca gcccggtctt gccgtccacg tggatcgctc
     1981 cctccccgat cgctcccgct cggagcagag gggcgaggcc cagcaccacg ggcgtgggga
     2041 agcatcccgg gttggctacg agcgcggcat cccggatgcg ggtggcgaac agctctggca
     2101 gcccgtacac ggccttgtcc agccaggccg gggccgggtg gatgaacccg taccagtccg
     2161 gatacgaccc ggcagggagc cggaagtcgc cggacaggtc gatcacgcgc agcccgaact
     2221 ccaccagcgg cggcgccaac cgggagctcg ccccgtgggg cagggccagg aacgcggcgt
     2281 ccgcgatccc ggccacggct gccggctcca tgggccccag cacctcgtcc ccgagcggcg
     2341 atgccgcgag gtgcgggtgg acctcggcca gccgccgccc ggccgaggtc tccgcgccga
     2401 cgaacgagac ggaggccgaa gggtgctcga ggagcagccg gatcagctcg ccgccggtga
     2461 cgccggaggc gccaagcacg gcgacgcgga acggagcgag ggaaggcatg gtgcataact
     2521 atgcgagtga tgcatgacgg cgtcaacctc ggccgggagg acgcgctgcg tcggtgagcc
     2581 tcgcgcctgg ggccatcagc cctcggggcg gtccagccgc acgtcggcca gcagggcctc
     2641 ggcctgctcc acggccatgg tcaaccaggt gcgctcctga cggccacgga tgtcctggcg
     2701 cggataggcg aactggttca gggtctcgtc cgatccgatc tggatgccct tggtcgcgag
     2761 gtacgcgccg atcgcgaact cgaacgtgaa caacgccgtc ccgtcctcga aggcctgcac
     2821 cgtgtaggtg gtgtaccggt tcggatgggc cgtgtacacg gcggcgcccc tgggggcccg
     2881 ctcggtggag ggtgcgaagc cccggtcgcg cagcgcatcg gcgaagtcca ccgctccact
     2941 gtacggcgca gggatcgaga gggcggaacc gacggctagg ggccgatcgg cacctcgtag
     3001 tgcgagcagg tgagctcccg gtccaccagg aactcatcgt cgcgcgggta gaagcgggcc
     3061 accgtcacgt cctcgccggc gaacgccttg accgactcca gggactccca tagggagagc
     3121 acgaggaact cctcacggtt cccatcggtc cggtgcaaca ccagcacgcc ctggttgccc
     3181 ggcgtggacc ggagcgccgc caccccggac tcctccagat acccgacgta cccctcggcg
     3241 cgctcgggcg ccgtccatcc ccgccacatc ctgccgatca tccagtcccc cctcgatgct
     3301 cgagcggccc gcatcgctac ctcagcgcgc gcaaccgctc ccccgccgcg ggcggctcgc
     3361 ccggcgccag cgacccgccc gccccggccc gggccaccga tccgcgtgcc ttgctcagct
     3421 gggatcggag ctcgtcggtc ccgttctgga tcatggccgc cgcccgcgcg aagcgattct
     3481 ccaccaccgc ctccatcgca tcgagtaagg cggctatccg ggccagcgcc gagcgcacgt
     3541 cgcccgggtc ggcgaagtgg ttgaccaggc tgtacaggaa caggagcacc ggcagcgcgg
     3601 tggaatacgg cacgatcacc accccgcggg cgaacgcgtc ggcgtgagcc ttgcggagta
     3661 cggcgtaggc tgcgtcgggc accgccgcca cggccacggg cgaggtcagg gccggatcca
     3721 ggtactgcgc cacctcgcgg gcacggagcg ccaccacacg ctccacctcc cggcctcgag
     3781 cctcccgctc ggccgattcg gcggactcca gcgcttccag ctcggccacc gccggccact
     3841 tcgagtccac gggcaggcgg cggccgtcgg ggagccgcag cgcgaactcc acgaccttgc
     3901 cgttcacccg gaagtcggtc tcgagcatgt ccggggggag ctcggccagg tgctggcgga
     3961 gcacgttctc gccggcgcgt cccctgccgc ttccgccggc caggaccgtg gacagccggc
     4021 ggatcacgcc ccaggcctcc gactcctggt cccggcgctc gcgctcccgc acggccagct
     4081 cctcgagcac ccggcgggcg ccgtccagcc gatcgcccag atgctccgcc tgccgatccc
     4141 gcgccgagga ggcgtcggcc agccgacgga gctcggcggc ctggacctcc agccgggcct
     4201 ccaggcgcgc ctccagccgt ccctggctcg gggacgctcc tcggcggagc gcgaacaccg
     4261 tcaagccgag ggcgatgccc acggccgctc ccacga
//
LOCUS       DASQQQ010000222        16191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_41990, whole genome shotgun sequence.
ACCESSION   DASQQQ010000222 DASQQQ010000000
VERSION     DASQQQ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 16191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16191
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41990"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /gene="amrS"
                     /locus_tag="VE646_08430"
     CDS             <1..765
                     /gene="amrS"
                     /locus_tag="VE646_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HZD80052.1"
                     /translation="TLADAAGPEAIAVAAERLGCRSVAFTYNDPVIFLEYAIDVADAC
                     RAHGIRTVAVTAGYAMPEARAELFAHVDAANVDLKGFSEDFYRRVCLGSLQPVLDTLR
                     YLRGETSVWIEITNLVIPGENDDERQIDAMTRWVVEHLGPDVPMHFTAFHPDFKMRDR
                     PPTPPATLTAARRIAMANGVRYAYTGNVHDVAGQTTRCHDCGAPLIERDGYDLLNWNL
                     GPDGRCRVCGTRCAGVFDAEPGRWGSRRRPVRLASP"
     gene            762..1565
                     /gene="amrB"
                     /locus_tag="VE646_08435"
     CDS             762..1565
                     /gene="amrB"
                     /locus_tag="VE646_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HZD80053.1"
                     /translation="MSAPSEPRPRPAVAAGTFYPAEAHRLRAAVDRLLASGGAPIGDP
                     PPAVIVPHAGYRYSGPVAASAYRLLRAAGDRVRRVALLGPAHFVPLGGAAVPGSPAWD
                     TPIGTAAIDPVLREVAAGAGAVIDDGPHEPEHALEVQLPFLMRLLGAGTTFLPVAVGR
                     AGAEVVAGLVEALWGEVDLVVVSTDLSHYLDVHAARLRDRRTADAVLARRAEAIGPED
                     ACGLFALRGIVQLARVRDLPIRLLDLRTSADTLGDPDRVVGYGAFAVGA"
     gene            1625..2836
                     /locus_tag="VE646_08440"
     CDS             1625..2836
                     /locus_tag="VE646_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD80054.1"
                     /translation="MALVGARRGTGTGTGRDVRLLFALGGATTAALLPFFVLWLRDRG
                     LTADRIGIVLAVSNLAGVLTAPAWGHVADTRLGSVGALRVASVGAALTALALVPTGSV
                     FALILLVSAVHATMESPGTALADAIALGHLGVDRATSYGSIRLWASAGWAVAVIAFGA
                     LFQRRGLGAVMPIYAGGMLLYALVLTARFQRIRPEGRGSHGTLVASWGALRSNRRLLI
                     FLAGLLVVSTATWGTWSFVPLRIASRGGGAFLVGLAAGLAAVVEIPFFRASAWLGRVA
                     GQRLLYAAGCGIYIAMMVGWALVPAPGVVAAIKVVGGAGFGLTYAALVVITGRLVPRE
                     LQATGQTLMQVTGNWIGPILGSAAGGFVYRHVGPPALFGGAAALLSVGVVVVWSSLRG
                     VDERGPAAAVR"
     gene            2866..3963
                     /gene="rlmN"
                     /locus_tag="VE646_08445"
     CDS             2866..3963
                     /gene="rlmN"
                     /locus_tag="VE646_08445"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZD80055.1"
                     /translation="MNVFDLSGEALGERLRAWGEPPFRGGQVYAQLWRRSATYEEMSD
                     VPPALRARLQAELPRAIDVLEERVADAGATRKALLLLGGRHVIETVLMGYPDRVTACV
                     SSQSGCAMGCGFCATGQMGLQNNLTVGEIAAQVVWARREAARLPPGTPRRLSNVVLMG
                     MGEPLANERHTFEAIQRLTDPTGMGMGDRHITLSTVGVAPGIRRLAERFPQVGLAVSL
                     HAADDELRDRLVPVNRLWPLRVLEGAIADWRARTHRRPSIEWAMIDGVNDGSGQAEML
                     TGIARRLHAHVNLIPLNPTPRSALRPSPPGRIGRFVEVLRVGGVNVTVRDTRGRRIEA
                     ACGQLRWAHEAAGRASRTAYRPEGAPAATSA"
     gene            complement(3978..5516)
                     /locus_tag="VE646_08450"
     CDS             complement(3978..5516)
                     /locus_tag="VE646_08450"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZD80056.1"
                     /translation="MRWRARSLPPGVRRGARIALPILIGLLGAWLGMALWSGTTVQMG
                     PFEVRLDSAFGRGVTDIELPPLGRLTADTHLAPLRLRATLTDVKIGELSRQVGDGGLD
                     QLVAGVESDAKHAVVLEALKVLVVTVLTAGVLALIVFRTRWRAVLTSVLACLLAVGGT
                     QVLAVGTFRPQAFGAPTFSGSLALAAQVIPPGRTVKELDAFQEQLRRVVDGAINAFTS
                     VQTNLVGRAGEIRVLHISDMHLSPLGFQFAQQIASGFDVDFVIDTGDTTSFGSEPENF
                     VLNYIRGFDRPVVWVRGNHDSLAFQRDLAKLPNTTVLDGRTTEVDGIRIYGLGDPVPP
                     TITRNLPDDVFAEQARLAGQRVLSDVEDSPPVGIVAVHDWRMAESVAGRVPLVLSGHF
                     HETKAWVQDGTLYLQIGTTGASALTTFETSANIPMSAEVLYFEPGAPPSLVAYDLIEQ
                     SPKTGQLTVTRHVIEDEFGHLSPSPTTPPPTPTPTPSATPTPSGPSPTASPSVSVSAS
                     PALP"
     gene            5779..7308
                     /locus_tag="VE646_08455"
     CDS             5779..7308
                     /locus_tag="VE646_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80057.1"
                     /translation="MEQTVSCTSSTPRTMALAGSVASVLLVAVVASVPGSPFTPILPR
                     GAGPFGPFRWIAVGVGLDRLSPSALAGVGLATMALAAGAFLLVLREAWRGTIGVRTLL
                     WLAVAYQLGLLLVPLLFSRDVYSYAAYGRIAGVHHANPYVLAPAAFPNDAFTPFVGPK
                     WVGTPAVYGPLFTLLSSGLVRVVAGVAATILVFRALAIAATLVSLSLIAWLVRRAQPA
                     RAAFAVAAFGLNPVVLFQSSGSGHNDLLVALAVVGALALVWARRELLATASLSLGALV
                     KVTAGVPLLLLIVAATARAEPGRRLRALASHLGLSLAIALVFAAPFLNASDPTLGMSE
                     LARHEGWLAPSRFFRRLLDALSGDTLGIAARVLFAAMLVASIAAIAVWLARRGAARGP
                     AAWGGAWAWGLLLLMLLGPVLLPWYAVWSLPLVWLLPRVPRASLIGTGVALTLSGWTA
                     EPSRFTRAYDLNVLFGHYVLTPIVILLLAWTGWDLWRRLREGRPLEDDQARVPAQAGQ
                     G"
     gene            complement(7243..9099)
                     /locus_tag="VE646_08460"
     CDS             complement(7243..9099)
                     /locus_tag="VE646_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80058.1"
                     /translation="MPGTPKDTTTERSSRTDAVLVAALTAVLLAWFAGPFVIRGIGFP
                     LGPDAPVYLWWTRLAGAEGLSAPERAGVPALALVLGGTLHLPLVAITAALEIVFGVGV
                     GLASAALLRAGRRDRVAWMLAGLLAGAFAVHLAAGYLANLVFAALFLASAATLAEGAH
                     RGPAAAAGLLAAAGFAHPLFFLLGILILLAAALLAWQADRAEARRVGAAVSGAGAVLG
                     IGLLALLAGPGPPAVDTSKDAFLRRAGLGADLRGAYLGRFLQHWPRYVQWASLPLAVM
                     GLLGTVGYVGRFLLAWGVALVVGVLAGLATGAFPADRFLTFGYVVPILAAIGVIRLWW
                     GLRRRPVLAWLAAVGLVAAMLGGALLAWSRQPPFIDRQQIAEVTVAGRYAAATPTGTP
                     LLFRVDSGDAATTFLATQAANLIRAALPPDRIRDVYVVVPPLTGSTALSPRNARERRA
                     LVFFYDRDAARAIRAAAQPPLLFDLLAFDAPGYGSELSCPELSCQGDRGPMQEVAPGV
                     RVDASHPAPVLAPVDPLGASSPADAVVAALAALALIGAVGYGWARTLTPDRPLALLLC
                     PLLGAAALMLSGIILERLGLPLTGWVGPTAVSALAGLSGYAGLIVLQRPALA"
     gene            9147..10532
                     /locus_tag="VE646_08465"
     CDS             9147..10532
                     /locus_tag="VE646_08465"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HZD80059.1"
                     /translation="MLDRELVHEVLQVARSRGGAFAEVYVEERSSIQIRLDDGKIEEL
                     TSGLDRGAGVRVAHGASYGYAFSNRLDRGALLEAAAAAAASVRQDGAGDVVDLRRAEP
                     PVVHTVERPASAVGAKDKVAWLREVDDTARSFHPEVRQVLASYADSLQRVLIATSDGR
                     WVEEERPRVRLFVTVVAARDGTIQTGTHGPAALSGLEFFRDHPPADTARVAARRAVTM
                     LDAGPAPAGEMPVVLAPGMGGVLFHEAVGHPLESDIVDKEASVYRGKVGRQVASTLLN
                     GVDDATVPNGWGSFSFDDEAAPAQRTVLFQDGVLQGWLYDRLRAEKDGVASSGNGRRQ
                     SYAHPPIPRMTNTYILDGADRAEDVIASTRRGVYVTALGGGQTNPATGDFVFGCSEAF
                     LIEDGEVRSPVRGANLIGRAIEVMSAVDAVAGDFDTWEGVCGKDGQGVPVGSGSPTLR
                     IGRITVGGTGA"
     gene            10525..11862
                     /locus_tag="VE646_08470"
     CDS             10525..11862
                     /locus_tag="VE646_08470"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HZD80060.1"
                     /translation="MPDLAELCAAAPEAAEGDEAVEAYAEESRRTEVTAHRAEVEGLT
                     FAESRGVGVRLIAEGRLGYAYAADPSIEDVRRAVQQARRNAGFATPDEHNGLPEARAV
                     EPVPWLFRQDQAHMDTERKVALALDLERLATTSDPRVSKVEQAQFGDALSRVAIASTE
                     GVSAEYARTDCWAVASVLAERDGETQTGWSFRVAREVSELEWADVASEAVSRGVRLLG
                     ATKPRSARVPVVLDRFAGSSFLGVLAGALSAEAVQKQRSLFAGMVGERVGSERFTLVD
                     DGRLLDGPAAAPFDDEGVPTGRTELVGRGTLNGFLHNTYTARRGGTVSTGNANRAGYR
                     SVPGVGTSNFYVEAGTTGVGEILRQAEGGVLIQDVSGVHSGANPISGEFSVGATGLRI
                     SGGAPGEPLREMTVASTIPEMLRGVAAVGDDLRFFTSVGLPTILIGEMTIAGI"
     gene            complement(11929..13137)
                     /locus_tag="VE646_08475"
     CDS             complement(11929..13137)
                     /locus_tag="VE646_08475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZD80061.1"
                     /translation="MSGWRATALVVVLAAVGAAGTLAVAAVQGMGGDLVDIAAYIVPA
                     LLVTALATAIARPLLVRASMHARLTAVATVGAAVALVNLFVLARTMFVSNHDATLVGV
                     LLLYSLGAGAGAALVVARISTAAVDRLAGTARALGEGDLDARVGELEAGPELRTLAGT
                     LDEMARRLQTAQERERQVEAVRRDLVTAVSHDLRTPLSSLRAMVEAIDEGVVDDVPSL
                     QRYAVEMRRSVNQLVAMVDDLFELVQLDAGAIEAETERARLDEIVRSALATVELQAEE
                     KRLSLVSNLEAAAADAPCSPRLARVLQNLLVNAVRHTPVDGTVRLDARRLPAGLELTV
                     EDTGEGIAPQHLGRVFEPFFRADPSRSGPGAGLGLALARRIVEALGGRIEAESEPAQG
                     SRFAVLLPLG"
     gene            complement(13134..13823)
                     /locus_tag="VE646_08480"
     CDS             complement(13134..13823)
                     /locus_tag="VE646_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD80062.1"
                     /translation="MAETRVLVVDDEPMVREVLARYLAKEGFAVDVAEDGEQALERFQ
                     TGPPDLVLLDLMLPRVDGLEVFRRMRELGASAVIMLTAKGEETDRVVGLELGADDYVT
                     KPFSPREVVARVRAVLRRAGVRITPVIRPVLTFGDLHIDGRRRELQRGGEPVHLTRKE
                     FDLLYFLASQPGITFSRSQLLEEVWDFAWDGDTATVTVHVRRLREKIEDDPSNPTHLM
                     TVWGVGYRFDP"
     gene            13982..14623
                     /locus_tag="VE646_08485"
     CDS             13982..14623
                     /locus_tag="VE646_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZD80063.1"
                     /translation="MPDVILPVLDEALALPWVLERLPVGYHPIVVDNGSRDGSPDIAR
                     SMGAELVRESRRGFGAACFAGLEAATTELVCFMDCDASLDPRHLPRVADPVLRGDAEL
                     VLGTRVAEPGAWPAHARLGNRALAGQVRRRTGISLGDLGPMRAARREALRDLRLRDRR
                     FGWPLEMVVRAAEAGWRIREVPVPYRARAGRSKVTGTVGGTLRAIRDMAAVLR"
     gene            14620..15276
                     /locus_tag="VE646_08490"
     CDS             14620..15276
                     /locus_tag="VE646_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019062713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HZD80064.1"
                     /translation="MRRSHLACLLVVAKAPVPGRSKTRLCPPCTPEGAARLAEAALAD
                     TLETVAATPVARRVLVLEGTAGRWLPGGFEVIAQRGEGLAERLANAFVDAGGPGLLIG
                     MDTPQVTPALLERGLCALRHPSTDAVIGPARDGGYWAIGLRRPDPEVFRDIPMSTPGT
                     LIAQRRRLRALGLRTARLPQRRDVDRIGDALAVAASSPRSRFARTLREVLTPPTEATA
                     "
     gene            15273..15902
                     /locus_tag="VE646_08495"
     CDS             15273..15902
                     /locus_tag="VE646_08495"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZD80065.1"
                     /translation="MSATVLGPAPKIAGRSRFVLRTEAGDLLPLHVERWFGEADPVEE
                     EVLERAVGPVLDIGCGPARHTIALVRRGVVAMGVDVSRTAVRAAAGRGASVVHRSVFE
                     PLPGEGSWGTALLLDGNVGIGGDPVRLLGRIGALLAPGGLVLAEVEAPGAPTGPLVVA
                     IEDADGVGPWFRWARVGIDGVSGLARDTGFGLRELWMAGGRWFTELVMT"
     gene            15956..>16191
                     /locus_tag="VE646_08500"
     CDS             15956..>16191
                     /locus_tag="VE646_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HZD80066.1"
                     /translation="MRLTPPRVGPFRAGAFRSRLHSERNAAWLGIALGVAFTTCFLTG
                     LLSHLIQHPPSWFLWPTRPAGTYRVTQGIHIVTG"
BASE COUNT         2032 a         5781 c         6154 g         2224 t
ORIGIN      
        1 acgttggccg acgccgctgg cccagaggcg atcgccgtgg ccgccgagcg gctggggtgt
       61 cgcagcgtgg ccttcaccta caacgatccg gtgatcttcc tcgagtacgc gatcgatgtg
      121 gccgacgcct gtcgcgcgca cggtatccgg accgtggccg tgacggccgg gtacgcgatg
      181 cccgaggctc gggccgagct gttcgcgcac gtggatgcgg ccaacgtgga cctcaagggg
      241 ttcagcgagg acttctaccg gcgggtctgc ctggggagcc tgcagccggt cctggacacg
      301 ctccggtacc tgcggggcga gaccagcgtg tggatcgaga tcaccaacct ggtgatcccg
      361 ggggagaacg acgacgagcg ccagatcgac gccatgaccc ggtgggtggt ggagcacctc
      421 gggcccgacg tgccgatgca cttcaccgcg ttccaccccg acttcaagat gcgggaccgg
      481 ccgcccaccc ccccggccac cctgaccgcc gcgcggcgga tcgccatggc caacggtgtt
      541 cggtacgcct acacgggcaa cgtgcacgac gtcgcggggc agaccacacg gtgtcacgac
      601 tgcggggctc ccctgatcga gcgggacggg tacgacctgc tgaactggaa cctcggcccc
      661 gatgggcgct gccgggtctg cggtacccgg tgcgccggcg tgttcgacgc cgagcccggt
      721 cggtggggca gccgtcgccg gccggtccgc ctggcctctc cgtgagcgct ccctccgagc
      781 cccggcctcg tcccgcggtc gccgccggca cgttctaccc ggcggaggcc caccgcctgc
      841 gggccgcggt ggaccggctc ctggcctcgg gcggcgcgcc gatcggggac ccgccgccgg
      901 ccgtcatcgt cccgcacgcc ggctaccggt actcggggcc ggtggccgca tccgcctacc
      961 ggctcctgcg ggcggcgggc gaccgggtgc gtcgggtggc gctgttgggt ccagcccact
     1021 tcgtgcccct ggggggtgcg gcggtgccgg gctcgcccgc gtgggacacc ccgatcggga
     1081 cggccgcgat cgacccagtc ctccgggagg tcgccgcggg cgccggtgcc gtcatcgacg
     1141 atggtcctca cgagcccgag cacgcgctgg aggtccagct tccgttcctg atgcgcttgc
     1201 tcggagccgg gaccacgttc ctgcccgtcg ccgtcggccg ggcgggggcc gaggtggtcg
     1261 ccgggctggt ggaggcgctc tggggggagg tcgacctggt ggtggtgagc accgatctca
     1321 gccactacct ggacgtccac gcggcccgcc tccgcgaccg ccggacggcc gacgcggtgc
     1381 tggcccgccg ggccgaggcg atcggcccgg aggacgcctg tggcctcttc gccctgcgcg
     1441 ggatcgtgca gctggcccgc gttcgcgacc tcccgatccg tctgctcgac ctccgcacgt
     1501 cggccgacac gctgggcgat cccgaccgcg tggtgggcta cggcgccttc gccgtcggag
     1561 cgtgaagctg cgacggcggc ccgatcgatc gccgggacga tagggtccgg gcgggggcga
     1621 acgcgtggca ctcgtcgggg cgaggcgggg aaccgggacc ggcaccggcc gggacgtgcg
     1681 cctgctgttc gcgctcggcg gggcgaccac cgcggccctg ctgccgttct tcgtgctgtg
     1741 gctgcgggat cgagggctga ccgcggaccg gatcgggatc gtgctggccg tctcgaacct
     1801 ggccggcgtg ctcaccgcgc ccgcctgggg gcacgtggcc gacacgcgct tggggtcggt
     1861 gggggccctg cgcgtggcct cggtgggggc ggcgctgacc gcgctcgcgc tggtccccac
     1921 gggctcggtg ttcgcgctga tcctgctcgt gtccgcggtc cacgccacga tggaatcgcc
     1981 cggcacggcg ctggcggacg ccatcgccct cggccacctg ggggtggacc gggcgacctc
     2041 gtacggctcg atccgcctat gggcgagcgc gggatgggcc gtggccgtga tcgcgttcgg
     2101 cgcgctcttc cagcggcggg ggctgggcgc ggtgatgccg atctacgcgg gtgggatgct
     2161 cctgtacgcg ctggtcctca ccgcgcggtt ccagcgcatc cggcccgagg gaagagggtc
     2221 ccacgggacg cttgtcgcct cctggggcgc gctgcggagc aaccgccgtc tgctgatctt
     2281 cctggccggg ctgctggtgg tgagcacggc cacctggggg acctggagct tcgtcccgct
     2341 gcggatcgcc tcccgcgggg gcggagcgtt cctggtgggc ttggcggcgg ggctggccgc
     2401 ggtggtggag atcccgttct tccgggccag cgcatggctc ggtcgggtgg ccgggcaacg
     2461 gctcttgtac gcggccgggt gcggcatcta catcgcgatg atggtcgggt gggccctggt
     2521 gccggctccg ggggtggtcg ccgcgatcaa ggtggtgggt ggcgcggggt tcggtctgac
     2581 ctacgcggcc ctggtggtga tcaccgggcg cctggtgccc cgggagctgc aggccaccgg
     2641 ccagaccctg atgcaggtga ccgggaactg gatcggcccc atcctcggct cggcggcggg
     2701 gggcttcgtc taccgacacg tgggcccgcc ggccctgttc ggtggggcgg ccgcgctgtt
     2761 gtccgtcggc gtggtggtcg tgtggtcctc cctgcgcggt gtcgacgaac ggggcccggc
     2821 tgccgccgtt cgttgaggga tgcatccccc ggtacgctga ccgggatgaa cgtgttcgat
     2881 ctgtcgggcg aggcccttgg cgaacgtctg cgcgcctggg gcgagccacc attccggggg
     2941 ggccaggtgt acgcacagct gtggcggcgt tcggccacct acgaggagat gagcgacgtc
     3001 ccgccggccc ttcgggcgcg gctgcaggcc gagctccctc gcgccatcga tgtgctcgag
     3061 gaacgtgtgg ccgacgccgg cgccacccgc aaggccctgc tcctgctcgg agggcgacac
     3121 gtgatcgaga cggtgttgat ggggtacccg gatcgcgtca cggcctgcgt gtcctcgcag
     3181 tccggatgcg ccatggggtg cgggttctgc gcgaccggac agatggggct gcagaacaac
     3241 ctcacggtgg gcgagatcgc cgcccaggtc gtctgggcca ggcgagaagc ggcccgcctc
     3301 cccccgggca cgccccggcg cctctccaac gtggtgctga tgggcatggg tgagcccctc
     3361 gcgaacgagc ggcacacctt cgaggcgatc cagcggctga ccgatccgac cgggatgggg
     3421 atgggggatc ggcacatcac gctctccacg gtcggggtag cccccggcat ccggcgcctg
     3481 gccgagcggt tcccgcaggt gggcctggcg gtcagcctgc acgccgcgga cgacgagctg
     3541 cgcgatcgtc ttgtgccggt gaaccgcctg tggccgctcc gcgtgctgga gggggcgatc
     3601 gccgactggc gggcgcgcac gcatcgccgc ccctcgatcg agtgggccat gatcgacggc
     3661 gtgaacgacg ggtccggcca ggcggagatg ctgacgggga tcgcccggcg gctgcacgcg
     3721 cacgtcaacc tgatccccct gaaccccacc ccccgctcgg cgctgcggcc gagccctccg
     3781 ggacgcatcg ggcgcttcgt cgaggtgctc cgcgtggggg gcgtgaacgt gacggtgcgg
     3841 gacacgcggg gccggcggat cgaggccgcc tgcgggcagc tgcggtgggc gcacgaggcg
     3901 gccggtcgtg cctcccggac cgcgtatcgc cccgagggcg cgccggccgc gacgtccgcg
     3961 tagccggggc ccaatcctca ggggagggcc ggcgaggccg agaccgacac gctgggcgag
     4021 gcggtcgggg aggggccgga gggcgtgggc gtggcggacg gcgtgggcgt cggggtgggc
     4081 gggggcgtcg ttgggctggg gctgaggtgg ccgaactcgt cctcgatcac gtgccgggtg
     4141 acggtgagct ggccggtctt cggggactgc tcgatcagat cgtaggccac caggctggga
     4201 ggggcgcccg gctcgaagta cagcacctcg gccgacatcg ggatgttcgc ggaggtctcg
     4261 aacgtggtga gggccgaggc accggtggtc ccgatctgca ggtacaacgt gccgtcctgg
     4321 acccaggcct tcgtctcgtg gaagtggccg gacaggacca acggcacccg ccccgccacc
     4381 gactccgcca tccgccagtc gtgcacggcc acgatcccga ccggcggcga atcttccacg
     4441 tccgacagga cgcgctgccc cgccaggcgc gcctgctcgg cgaacacgtc gtcgggcagg
     4501 ttgcgcgtga tcgtgggagg gaccgggtcc ccgaggccgt agatgcggat cccgtccacc
     4561 tctgtggtcc gcccgtccag gaccgtggtg ttggggagct tggccaggtc ccgctggaag
     4621 gcgagcgagt cgtggttgcc ccgcacccag accacgggtc gatcgaagcc gcggatgtag
     4681 ttgagcacga agttctccgg ctcggagccg aacgacgtgg tatcgcccgt gtcgatcacg
     4741 aagtccacgt cgaaccccga ggcgatctgc tgcgcgaact ggaagccgag cggtgacaga
     4801 tgcatgtcgg agatgtggag cacccggatc tcgcccgccc gtccgacgag gttcgtctgc
     4861 accgacgtga acgcgttgat ggcgccgtcc accacccgtc ggagctgctc ctggaacgcg
     4921 tcgagctcct tcaccgtgcg ccccggtggg atcacctgcg cggccagggc cagggagccc
     4981 gagaacgtgg gcgcgccgaa ggcctggggg cggaacgttc ccacggccag gacctgggtc
     5041 ccgcccaccg ccagcaggca ggccaggacg ctggtgagga cggcgcgcca cctggtgcgg
     5101 aacacgatca gcgccagcac cccggcggtg agcacggtca ccaccagcac cttgagcgcc
     5161 tccagcacca cggcgtgctt ggcgtccgac tccacgccgg ccaccagctg gtccagcccg
     5221 ccgtcgccca cctgccggct gagctcgccg atcttcacgt cggtcagggt ggcccgcagc
     5281 cgcagcggtg ccaggtgcgt gtcggccgtg agtcgtccga gcggcggcag ctcgatatcg
     5341 gtgacgcctc gcccgaaggc cgagtcgagc cgcacctcga acggccccat ctggaccgtg
     5401 gtccccgacc acagggccat gccgagccac gccccgagca gcccgatcag gatcgggagg
     5461 gcgatccgag cgccgcgacg gacgccgggt ggaagggatc gggcacgcca acgcatccca
     5521 gatggaacgt accagcgggg acccccatcc ccgggatccg gggccccgga tctcgcggat
     5581 cccggcttcc acggggtccc ggtccggccg ttgttcgagc gacggatgcc cgcctgggca
     5641 gggccggtgg gccctaggcc tgcggcaccg ccgcgggcag gctgacgccg tggtccggcg
     5701 cgacgggcgg accttgcccc ccgctcgtcc gccggccacc ggccttggcg cctgaggacc
     5761 gctgctacag tcggcccggt ggagcagacc gtgtcgtgca cctcgtccac gccgcggacc
     5821 atggctctcg ccgggtcggt ggcctcggtc ctgctcgtcg cggtggtggc ctcggtcccg
     5881 ggctccccgt tcaccccgat cctgccgcgg ggggccgggc ccttcgggcc gttccggtgg
     5941 atcgccgtgg gggtgggcct ggatcgcctc tcaccctccg cgctggccgg cgtgggcctg
     6001 gcgacgatgg cgctggccgc cggggccttc ctgctggtcc tgcgggaggc gtggcgcggg
     6061 acgatcgggg tccgcaccct gctgtggctg gccgtggcct atcagctggg gctgctgctg
     6121 gtgcccctgc tcttctcccg cgacgtctac tcgtacgcgg cctacggccg catcgccggt
     6181 gtgcaccacg cgaacccgta cgtgctcgcc ccggcggcgt tcccgaacga cgccttcacg
     6241 cccttcgtgg ggccgaagtg ggtgggcacg ccggcggtct acggacctct gttcacgctg
     6301 ctgtcatcgg ggctggtgcg ggtcgtcgcc ggcgtggcgg ccaccatcct ggtcttccgg
     6361 gccctggcca tcgcggccac gctggtgagc ctctccctca tcgcgtggct ggtccgccga
     6421 gcccagcccg ctcgagcggc gttcgccgtg gcggcgttcg ggctgaaccc cgtggtcctg
     6481 ttccagtcca gcggcagcgg tcacaacgac ctcctggtgg ccctcgcggt ggtgggggcg
     6541 ctggcgctgg tctgggctcg gcgagagctg ctggccacgg cgtcgctgtc gctcggggcg
     6601 ctggtgaagg tcaccgcggg ggtgccgctg ttgctgttga tcgtcgccgc cacggcgagg
     6661 gcggagcccg gccgtcgcct ccgggccctg gcctcgcacc tgggactcag cctcgcgatc
     6721 gcgctggtct tcgccgctcc cttcctgaac gcgagcgacc ccacgctcgg catgagcgag
     6781 ctcgcccgcc acgagggctg gctggccccg tcccgcttct tccggcggct gctggacgcg
     6841 ctgagcggtg acaccctggg gatcgcggcc agggtcctgt tcgccgcgat gctggtggcg
     6901 tcgatcgcgg cgatcgccgt gtggttggct cggcggggag cggcgcgggg gccggccgcc
     6961 tggggtggcg cctgggcctg gggcctgctc ctgttgatgc tgctggggcc ggtcctgctg
     7021 ccctggtacg cggtctggtc gctccccctg gtctggctac tgccccgggt gccgcgggcc
     7081 tccctgatcg gcaccggcgt ggccctgacc ctctccggat ggacggccga gccctcccgc
     7141 ttcacccggg cctacgacct caacgtcctg ttcggccact acgtgctgac ccccatcgtg
     7201 atcctcctgc tggcctggac cgggtgggac ctgtggcgtc gcctacgcga gggccggccg
     7261 ctggaggacg atcaggcccg cgtacccgct caggccggcc agggctgaga ccgccgtggg
     7321 gccgacccac ccggtcaggg gcaggcccag ccgctccagg atgatgccgc tcagcatgag
     7381 cgccgcggcc ccgagcaggg gacagagcag gagcgccagg ggccggtccg gcgtgagggt
     7441 ccgggcccag ccgtagccga ccgcgccgat cagggcgagc gccgcgaggg ccgcgaccac
     7501 cgcgtcggcg ggcgacgagg ccccgagggg atccacgggg gcgaggaccg gcgccggatg
     7561 cgacgcgtcg acccggaccc cgggagcgac ctcctgcatc ggaccgcggt cgccctggca
     7621 gctcaactcc gggcaggaga gctcggaccc gtacccgggg gcatcgaagg ccaggaggtc
     7681 gaacagcagg ggcggctggg cggcagcccg gatggcgcgg gccgcgtccc ggtcgtagaa
     7741 gaagaccagg gcccggcgct cccgtgcgtt ccggggggac agggcggtcg acccggtgag
     7801 cggtgggacc accacgtaga catcgcggat ccggtccgga ggaagcgcgg cacggatcag
     7861 gttcgcggcc tgcgtggcca ggaaggtggt ggccgcgtcg cccgagtcca cgcggaacag
     7921 caggggcgtg ccggtcgggg tggcggccgc gtaccgcccg gcgaccgtga cctcggcgat
     7981 ctgctggcgg tcgatgaacg gcggttgccg ggaccacgcg agcagcgcgc cgcccagcat
     8041 cgccgccacc agccccacgg ccgccagcca ggccagcacg ggccgtcggc ggagtcccca
     8101 ccacaggcgg atcacgccga tggcagcgag gatcggcacc acgtagccga aggtgaggaa
     8161 ccggtcggcg gggaaggcgc cggtggccag cccggccagc acccccacca ccagcgccac
     8221 cccccaggcc agcaggaacc gtcccacgta gcccaccgtc ccgagcagtc ccatcaccgc
     8281 gaggggcagg gaggcccact ggacgtatcg gggccagtgc tggaggaagc ggcccaggta
     8341 cgcgccgcgc aggtccgcac ccaggccggc ccggcgcagg aacgcgtcct tcgaggtgtc
     8401 caccgccggg ggaccgggcc cggccagcag cgccagcagg ccgatcccca gcaccgcgcc
     8461 ggccccgctc accgcggccc cgacccggcg cgcctcggca cggtcggcct gccacgcgag
     8521 cagcgccgcc gccagcagga tcaggatgcc gagcaggaag aacagcggat gggcgaaccc
     8581 ggcggccgcg agcaggcccg cggcggccgc cggcccccgg tgtgcaccct cggccaaggt
     8641 cgccgccgag gcgaggaaca gcgccgcgaa caccaggttg gccaggtagc cggcggccag
     8701 gtgcacggcg aacgcgccgg cgagcagccc cgccagcatc cacgcgaccc ggtcccgccg
     8761 gccggcgcga aggagcgccg ccgaggccag gcccacgccc accccgaaca cgatctccag
     8821 ggcggcggtg atcgccacca gcggaaggtg cagcgtcccc ccgaggacca gcgccagggc
     8881 cgggaccccc gcgcgctcgg gcgccgacag tccctccgcc cccgccaggc gcgtccacca
     8941 caggtacacg ggcgcgtcgg gcccgagggg gaacccgatc ccccggatca cgaagggacc
     9001 ggcgaaccag gccaggagca ccgcggtcag ggccgcgacc agcacggcat cggtgcggga
     9061 cgagcgttcc gtggtcgtgt ccttcggtgt gcccggcaac ggtggcggga ctgtaacaac
     9121 cggtcgcttg gtacgattcg ccatccatgc tcgatcgcga actggtccac gaggtgctgc
     9181 aggtggcgcg gtcgcgcggc ggtgccttcg ccgaggtcta cgtggaggaa cgcagttcga
     9241 tccagatccg gctggacgac ggcaagatcg aggagctcac cagcggcctg gatcgcgggg
     9301 cgggagtgcg tgtggctcac ggcgcctcgt acggctacgc cttctccaac cgcttggatc
     9361 ggggggccct gctggaggcc gccgcggcgg cggcggcctc ggtgcgccag gacggcgcgg
     9421 gtgacgtggt ggacctccga agggcggagc ctccggtggt gcacacggtg gagcggcccg
     9481 cctcggccgt gggggcgaag gacaaggtgg cctggctgcg cgaggtcgac gacaccgccc
     9541 gctccttcca tcccgaggtc cgtcaggtgc tggcgagcta cgcggactcc ctgcagcgcg
     9601 tgctgatcgc cacctcggac ggccgctggg tggaggagga gcgcccgcgc gtccgcctgt
     9661 tcgtgacggt ggtcgccgcg cgcgacggga cgatccagac cgggacccac gggccggcgg
     9721 cgctgtccgg cctcgagttc ttccgggacc atccgccggc ggacacggcc cgtgtcgccg
     9781 cccggcgcgc ggtgaccatg ctcgacgccg gccccgcgcc ggcgggggag atgccggtgg
     9841 tcctggctcc ggggatgggc ggcgtgctgt tccacgaggc cgtgggccac ccgctcgagt
     9901 ccgacatcgt ggacaaggag gcctcggtgt accgggggaa ggtgggccgg caggtggcct
     9961 cgacgctcct gaacggcgtg gatgatgcca ccgtgccgaa cgggtggggc tcgttctcct
    10021 tcgacgacga ggcggcaccc gcccagcgca cggtcctgtt ccaggacggg gtcctgcagg
    10081 gctggctcta cgatcggctg cgggccgaga aggacggcgt ggcctccagc ggcaacggac
    10141 ggcgccagag ctacgcccat ccccccatcc cgcggatgac caacacgtac atcctggacg
    10201 gggcggaccg ggccgaggac gtcatcgcct ccacccggcg tggcgtctac gtcacggcgc
    10261 tcgggggtgg gcagacgaac ccggccaccg gggacttcgt gttcggctgc tccgaggcgt
    10321 tcctgatcga ggacggcgag gtccgcagcc cggtgcgggg cgcgaacctg atcgggcggg
    10381 ccatcgaggt gatgtcggcc gtcgacgcgg tggcgggaga cttcgacacg tgggagggcg
    10441 tgtgcggcaa ggatgggcag ggcgtgccgg tggggagcgg ctcgcccacc ctgcggatcg
    10501 gccgcatcac cgtggggggc accggtgcct gatctggccg agctgtgcgc cgccgccccg
    10561 gaggccgccg agggggacga ggccgtggag gcctacgcgg aggagtctcg ccggaccgag
    10621 gtcacggcgc accgcgcgga ggtggagggc ctcaccttcg ccgagtctcg cggcgttggc
    10681 gtccggctga tcgccgaggg ccgtctgggg tacgcctacg ctgccgatcc ctcgatcgag
    10741 gacgtccgtc gcgccgtgca gcaggcccgg cgcaacgccg gcttcgccac gcccgatgag
    10801 cacaacgggc tccccgaggc ccgtgcggtg gagcccgtcc cctggctgtt ccggcaggat
    10861 caggcccaca tggacaccga gcgcaaggtg gccctggccc tggacctgga acggctggcc
    10921 accaccagcg acccacgcgt gagcaaggtc gagcaggcgc agttcgggga tgccctgtcc
    10981 cgggtggcga tcgcctccac cgagggcgtg tccgccgagt acgcgcgcac cgactgctgg
    11041 gcggtggcct cggtcctggc cgagcgggac ggggagaccc agaccggatg gtcgttccgg
    11101 gtggcccgcg aggtgagcga gctcgagtgg gccgacgtgg cctccgaagc cgtctcccgc
    11161 ggggtccggc tgctgggggc cacgaagccc cgatcggctc gggtccccgt ggtgctcgac
    11221 cggttcgccg gctcctcctt cctgggcgtg ctggccgggg ccctgtcggc cgaggccgtg
    11281 cagaagcagc gatcgctgtt cgccgggatg gtgggggagc gggtgggctc cgagcgcttc
    11341 accctggtgg acgacggccg gctcctggac ggtccggcgg ccgctccctt cgacgacgag
    11401 ggcgtgccca ccggccggac cgagctggtc ggccggggga cgctgaacgg cttcctgcac
    11461 aacacctaca cggcgcgacg gggcggcacc gtgtccaccg gcaacgcgaa ccgtgccggc
    11521 taccgctcgg tgccgggtgt gggcacctcg aacttctacg tggaggccgg caccaccggc
    11581 gtgggggaga tcctccggca ggccgagggg ggcgtgctga tccaggacgt ctccggggtc
    11641 cactccggag ccaaccccat cagcggggag ttctcggtgg gggcgacggg gctgcgcatc
    11701 agcggtgggg cgccagggga accgctccgg gagatgaccg tggcctccac gatccccgag
    11761 atgctgcggg gcgtcgcggc ggtgggcgac gacctccgct tcttcacctc ggtcggtctg
    11821 cccaccatcc tgatcggcga gatgaccatc gccgggatct gagctgcccc gcagcacctg
    11881 ccgagcgcac gagccgctca gaccagctgg tgcgccaccc gggggaagtc accccagggg
    11941 aagcagcacc gcgaagcgag agccctgcgc cggttcgctc tccgcctcga tccggccccc
    12001 gagggcctcc acgatccgcc tggccagggc caggccgagc cccgcgccgg ggccggaccg
    12061 ggaggggtcg gcccggaaga atggctcgaa cacccgcccc agatgctgcg gcgcgatgcc
    12121 ctcccccgtg tcctccaccg tgagctccag accggccgga agccggcggg cgtcgagccg
    12181 gaccgtcccg tccacgggcg tgtgtcgaac ggcgttcacc aggaggttct gcaggaccct
    12241 ggccagccgg ggcgagcacg gagcatcggc cgccgccgcc tcgaggttgg acaccagcga
    12301 gagccgcttc tcctccgcct gcagctccac ggtggccagg gccgagcgga cgatctcgtc
    12361 cagccgggcg cgctcggtct ccgcctcgat ggccccggcg tccagctgga ccagctcgaa
    12421 caggtcatcg accatggcca cgagctggtt cacggatcgg cgcatctcga cggcgtagcg
    12481 ctgcaggctg gggacgtcgt ccaccacccc ctcgtcgatc gcctccacca tcgcccgcag
    12541 gctggagagc ggggtccgga ggtcatgcga gaccgcggtg accaggtcgc ggcggacggc
    12601 ctcgacctga cgctcgcgct cctgggccgt ctgcagccgc cgagccatct cgtcgagcgt
    12661 gccggccagc gtccgaagct cgggccccgc ctccagctcg cccacccggg cgtccaggtc
    12721 gccctccccg agcgcccggg ccgtgccggc cagccgatcc acggccgcgg tggagatccg
    12781 ggccaccacc agcgccgccc ccgccccggc tcccagcgag tacaggagga ggacccccac
    12841 cagcgtggcg tcgtggttcg agacgaacat cgtgcgggcc aggacgaaca ggttcaccag
    12901 ggccaccgcg gcccccaccg tcgccaccgc cgtgaggcgt gcgtgcatcg aggccctgac
    12961 caggaggggg cgggcgatgg ccgtggccag cgcggtgacg agcaacgccg ggacgatgta
    13021 cgcggcgatg tcgaccaggt cgccgcccat gccctggacc gcggccacgg ccagcgtgcc
    13081 cgccgcgccc accgcggcca gcaccaccac cagcgcggtc gcccgccagc cgctcatgga
    13141 tcgaaccggt agcccacgcc ccacaccgtc atcaggtggg tggggttgga ggggtcgtcc
    13201 tcgatcttct cgcgcagccg acgaacgtgg acggtgaccg tcgccgtgtc gccgtcccag
    13261 gcgaagtccc agacctcctc cagcagctgc gagcggctga acgtgatccc gggttgggag
    13321 gccaggaagt acaggaggtc gaactccttg cgcgtgaggt gcaccggttc ccctccccgc
    13381 tgcagctcgc gccgtcggcc gtcgatgtgc aggtccccga aggtcaggac gggacggatc
    13441 accggggtga tccgcacccc ggcccgccgc agcacggctc ggaccctcgc caccacctcc
    13501 cgaggggaga acggcttggt gacgtagtcg tcggccccaa gctccagccc caccacccgg
    13561 tcggtctctt cccccttggc ggtgagcatg atcaccgcgg aggcccccag ctcgcgcatc
    13621 cgccggaaga cctccagccc gtccacgcgg ggcagcatca gatccaggag gaccagatcc
    13681 gggggtccgg tctggaaccg ctccagcgcc tgttccccgt cctcggcgac gtccacggcg
    13741 aagccttcct tcgccaggta gcgcgccagg acttcacgca ccatgggttc gtcgtccacc
    13801 accaggaccc gtgtctcggc cacgtcccca tgatgaaccg aagtccttac cagatccttt
    13861 ccctcgtccc ggatccgagc agggcggccg aagtttcagc gtccgttccg ggttctgaaa
    13921 ggaacacgta aggaaccagg ccggacactg aggtcatgcg ttggacggtc cggtgatccc
    13981 ggtgcccgat gtgatcctgc cggtgctgga tgaggcgctg gccctgccct gggtgctcga
    14041 gcgcctcccc gtgggctacc acccgatcgt cgtggacaac ggctcgcgcg acgggtcgcc
    14101 ggacatcgcg cgatcgatgg gcgcggagct cgtccgggag tcgcggcggg ggttcggggc
    14161 cgcctgcttc gccggcctgg aggccgccac gaccgagctc gtgtgcttca tggattgcga
    14221 cgcctcgctg gacccccggc acctgccccg ggtggccgat ccggtgctcc ggggagatgc
    14281 cgagctcgtc ctgggcaccc gggtcgccga gccgggtgcc tggccggccc acgcccgact
    14341 gggcaaccga gccctggccg ggcaggtgcg ccgacgcacg gggatctcgc ttggcgatct
    14401 gggcccgatg cgggccgccc gtcgagaggc actccgggat cttcggctgc gagatcgacg
    14461 cttcggatgg ccgctggaga tggtggtgcg agccgccgag gccggatggc ggatccggga
    14521 ggtccccgtg ccgtaccggg ctcgcgccgg ccgttcgaag gtgaccggca cggtgggggg
    14581 aacgctgcgg gcgatccggg acatggcggc ggtgctccga tgaggcgatc gcacctcgct
    14641 tgcctcctgg tggtcgccaa ggctcccgtc cccggccggt ccaagacgag gctctgccct
    14701 ccgtgcacgc cggaaggggc cgcacggctg gcggaggccg ccctggcgga cacgctcgag
    14761 accgtggccg ccaccccggt cgctcgccgg gtgctggtcc tcgagggcac ggcgggacgc
    14821 tggctgccgg gcgggttcga ggtgatcgcc cagcgcggcg aggggctcgc cgagcggctg
    14881 gccaacgcgt tcgtggacgc cgggggcccg ggcctgctga tcgggatgga cacgccccag
    14941 gtgacgcccg cgctgctgga gcgcggcctg tgcgcgctgc ggcacccgtc caccgacgcg
    15001 gtgatcggtc cagctcggga cggtggatac tgggcgatcg ggctccgacg ccccgacccg
    15061 gaggtcttcc gggacatccc gatgagcacg ccgggcacgc tgatcgccca gcggcgacgg
    15121 ctgcgcgcgc tcgggctgcg gacggctcgc ttgccgcagc gccgagacgt ggaccggatc
    15181 ggggacgccc tggcggtggc ggcgtcgtcg ccgcgctccc ggttcgcgcg gaccctgcgc
    15241 gaggtcctga ccccgccgac cgaggcgacc gcatgagcgc gaccgtcctg ggcccagcac
    15301 cgaagatcgc cggtcggtct cggttcgtgc tgcgtacgga ggccggcgac ctgctgcccc
    15361 tgcacgtgga gcgctggttc ggcgaggcgg accccgtcga ggaggaggtg ctcgagcggg
    15421 ccgtgggacc ggtgctcgat atcggttgcg gtcccgcccg ccacacgatc gcgctggtgc
    15481 gccggggcgt cgtggcgatg ggtgtcgacg tgtccaggac ggcggtgcgg gccgccgccg
    15541 gcaggggcgc ttcggtggtg caccggtcgg tgttcgaacc gttgccgggg gaggggagct
    15601 ggggcacggc gctgctgctc gatgggaacg tggggatcgg tggggacccg gtgaggctgc
    15661 tggggcggat cggcgccctg ctcgccccgg ggggcctggt gctggccgag gtggaggcgc
    15721 ccggcgcacc gaccggtccg ctggtggtgg cgatcgagga cgcggacggc gtggggccct
    15781 ggttccgatg ggcccgggtg ggcatcgacg gcgtgagcgg cctggcccgg gacacggggt
    15841 tcgggctgcg ggagctctgg atggcgggcg gacggtggtt caccgagctg gtgatgacgt
    15901 gagcgatcgc accggctccg tgcgggggct gctcggttcg gtggaccgag ccctcctgcg
    15961 gctgacgccg ccgcgggtgg ggccgttccg ggccggggcg ttccgcagcc ggctgcactc
    16021 cgagcgcaac gccgcgtggc tggggatcgc gctgggtgtc gcgttcacca cgtgcttcct
    16081 cacgggcctg ctctctcacc tgatccagca cccgccgtcc tggttcctgt ggcccacgcg
    16141 gcccgccggc acgtaccggg tcacccaggg gatccacatc gtcaccggca t
//
LOCUS       DASQQQ010000223         7997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_314239, whole genome shotgun sequence.
ACCESSION   DASQQQ010000223 DASQQQ010000000
VERSION     DASQQQ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7997
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314239"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..728
                     /gene="rplJ"
                     /locus_tag="VE646_08505"
     CDS             39..728
                     /gene="rplJ"
                     /locus_tag="VE646_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HZD80067.1"
                     /translation="MPNAEKIDKVAALKERIQGSEALLLTEFRGLSVHDATELRRSLA
                     DTARFSIVKNTLMKRAAGDAGVAELGALLEGPTAVAFVSEDAVTAAKRLVDAARRYPA
                     LVLKGAFMEGKVLSAEQARALAELESREVMLSQVAGMLKNEMARAASMFQALQGRFLG
                     LLEAFKEKVPGEEAAPATDAAEADAAEADAADAETPAEVGPESQEPTGEATEETSSPE
                     AAQADGEEGEE"
     gene            732..1118
                     /gene="rplL"
                     /locus_tag="VE646_08510"
     CDS             732..1118
                     /gene="rplL"
                     /locus_tag="VE646_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006130601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HZD80068.1"
                     /translation="MAKMSAEDLLDQFKDMTLLELSDFIKKFEETFDVQAAATGPMML
                     APGAGPAAVEEVEEKTEFDVVLAAAGEKKIQVIKEVRALTSLGLKEAKDLVDGAPKPV
                     LEKVNKETADKAKEQLESAGATVEVK"
     gene            1251..1679
                     /locus_tag="VE646_08515"
     CDS             1251..1679
                     /locus_tag="VE646_08515"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZD80069.1"
                     /translation="MKDGRTFGQRLRETRIQAGMSQSDLEELSGIPKARLSRYENGHV
                     APSIQTLERLARALQVSEASLLGDQRAVLEEFFAVLTERGVVIQSTEQAKAVANAMAD
                     LFLSLVPREVPPSVEAGEPALGHEMPAPALSGSGGWLDGD"
     gene            1684..3027
                     /locus_tag="VE646_08520"
     CDS             1684..3027
                     /locus_tag="VE646_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HZD80070.1"
                     /translation="MSAPAEDLPAAAPPTTSWRSALHERLRSRTARVGVVGLGYVGLP
                     LLVSAVRAGFTGVGLDLDPERVEAVRSGRSYLSDVPDHVLGELADSLTPSTRPSVLRS
                     CDVITICVPTPLKDHDPDLTFIERAGHQVGRYLRPGTLAILESTTYPGTTDEILRPLL
                     ERRGMVAGRDFALAYAPERIDPGRGLEHVRATPRVVGGYTSACGKLARAFYASFVDQV
                     HQVASTREAEMAKLIENTYRHVNIALMNELAFLSRDLDVDIWEAIRAAATKPFGFQAF
                     WPGPGVGGHCIAIDPSYLSWRVGQRTGHRLNFVEHAQEVNARMPGFVVQRIGMALNDR
                     GKPLRGARVLGIGVTYKPDVSDLRESPSLVVLELLAAAGARVSYHDPLVPQLGLGGRT
                     LRSKPLVPRVLGQQDCVVVLTPHSGIDFREVADRSDLVFDTRGVTPLGHRRAVRL"
     gene            3149..4006
                     /locus_tag="VE646_08525"
     CDS             3149..4006
                     /locus_tag="VE646_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HZD80071.1"
                     /translation="MDPVFGSASELATFGAESFTPTVLFESERMKVVLAGLEADQRIE
                     LHAPQVDLAVAVLEGLGDLWVDDRPRPVRPGDVAVIPAGSTRGVRARGGRLVLLHVVS
                     PPPSAADHQVEHRAWPSEEAGPEVRAVLHQEHQELLPHLGHLGALAEQAPDLDEATLR
                     ERLHGVVDFLTHSLLPHAGVEEQTLYPAVDGLLRATGGGTGTMSIDHAEIGERIQALA
                     AMVEEPLNPSARTAIVRALEQLEAIVRLHFRKEEEAYLPLLSRLSAAEASELSDALSA
                     LGAEHHHEG"
     gene            complement(4052..5581)
                     /locus_tag="VE646_08530"
     CDS             complement(4052..5581)
                     /locus_tag="VE646_08530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HZD80072.1"
                     /translation="MVTSSVHRDIRYGARLLAFVRAAPERARERQFWLIQAQVAVVTL
                     LHLVVESSWGHELAASLALLHLPVVFYAVPITYAGFEYGLEGATLTCLWSAVLVVPNI
                     LLFHHVSIAGLGDAIFMLVVAILGTLVALAVERERRERLRAESTSQRLVLLNEVSAIL
                     EQERELRTGLSRALTRLVEVVGLRGAGLLLVGPDGEEGPRVCVPPASELRDPLLGACE
                     EAIGSAERSPGERDGGAVRVFPLQVEGTSVGCLAVSADPGQGLREEDEGLLSAAASQI
                     GAVADNVRLHERERERLHSYVRQVVAAQEDERARIARDLHDETAQELVVMRRELDELI
                     ASGDGLPGRVADRLEDLRTRSGSALSGLRRFGRDLRPALLDDLGLAPALEWLVEDMGN
                     RTGIGAGFRVHGQVRRLAPEVEVALFRITQEALRNVERHAGASRVFVDMQFDHGSASI
                     SVEDDGRGFRRRPTDAFARRGHLGLLGMEERARLIGAAMSIWSQPGSGSRVKVWLEPE
                     P"
     gene            complement(5581..6240)
                     /locus_tag="VE646_08535"
     CDS             complement(5581..6240)
                     /locus_tag="VE646_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD80073.1"
                     /translation="MGTRVILADDHTVVREGTREILERHGGIEVVGEASDGEEAVRLV
                     SALRPDVAILDLGMPKMGGLEAVRRIKATCPEVGVLVLTVHDEDQYVFAVLEAGAAGY
                     LLKDVPSERLIEAVEAVGVGEPVLHPTVVRKVLGRLQPDGDEGTDRSPRSLTDRELGV
                     LRLAARGMSNKEIARSMDLSARTVQVHLAHIFSKLQVASRTEAVVRALREGWFSLEEL
                     A"
     gene            6492..7475
                     /locus_tag="VE646_08540"
     CDS             6492..7475
                     /locus_tag="VE646_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZD80074.1"
                     /translation="MQSGDRPTGPPTTASGTDSVQHGMSRRRFLGYAGALTGVATGGL
                     LPWAERADAAEARDTAIRVHRWAMVIDLRRCDGCSGIGLPPQCTQYCIWGHLAPQGMQ
                     WIEVFDTTQSNLPGAPGRFLPAPCMQCQNAPCANVCPVGATFHTPEGTVLIDQERCIG
                     CRLCMAACPYDRRFFNWGEPVQPEYATDQPYDVLDQVPAIKGTVMKCDFCTDLAEAGG
                     ISFCADACPQGAIYYGDLEEDVATNGVDFVRLSTFIEENHATPYKEELGTEPRVFYIA
                     GDGELASESSADPRDREFLKGNLEWPWARIQQALEGQAPVGASSTQGGSDA"
     gene            7468..>7997
                     /gene="nrfD"
                     /locus_tag="VE646_08545"
     CDS             7468..>7997
                     /gene="nrfD"
                     /locus_tag="VE646_08545"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HZD80075.1"
                     /translation="MLDPAADHLERVGLAPLRRTTWRYWLLLAFLAAVAGLGFYAYAD
                     QLSKGLSVTGMRVSLNKIIWGLYITNFVFFIGISHAGTLISAILRVSKAGWRTPITRM
                     AEFITVVALLVGGLFPLIDMGRPDRVLNLLRFGRWQSPILWDVLAIATYLTGSLIYLY
                     LPLIPDLATCRDLIGGG"
BASE COUNT         1172 a         2927 c         2772 g         1126 t
ORIGIN      
        1 ctcgtctgcg ggggcgtttc cctatgcgag gagcaccgat gccgaacgca gagaagatcg
       61 acaaggtcgc ggccctcaag gagcgaatcc agggctccga ggccctcctc ctcaccgagt
      121 tccgaggcct gagcgtccac gacgccaccg agctccgccg gtccctggcc gacacggcca
      181 ggttctcgat cgtgaagaac acgctgatga agcgggccgc cggcgacgcc ggggtggccg
      241 agctgggggc cctgctggag ggccccaccg ccgtggcctt cgtctccgag gacgccgtca
      301 ccgcggccaa gcgtctggtc gatgcggcca ggcggtaccc ggccctggtc ctgaagggtg
      361 ccttcatgga gggcaaggtc ctctctgccg agcaggcccg ggccctggcg gagctggagt
      421 cgcgcgaggt catgctctcc caggtggccg ggatgctgaa gaacgagatg gcccgggccg
      481 cctcgatgtt ccaggcgctg cagggccggt tcctgggcct gctcgaggcc ttcaaggaga
      541 aggtcccggg cgaggaggcc gcgccggcca ccgacgctgc cgaggccgac gccgccgagg
      601 ccgacgccgc cgacgccgag acaccggccg aggtcggccc cgagagccag gaacccaccg
      661 gcgaagcaac cgaagagaca agcagcccgg aggccgcgca ggccgacggc gaggaaggag
      721 aggagtaacc gatggcgaag atgagcgccg aggacctgct cgaccagttc aaggacatga
      781 cgctgctcga gctctcggac ttcatcaaga agttcgagga gaccttcgac gtgcaggccg
      841 cggcgaccgg ccccatgatg ctcgcaccgg gcgccggtcc ggccgcggtc gaggaggtcg
      901 aggagaagac cgagttcgac gtggtcctgg ccgcagccgg cgagaagaag atccaggtca
      961 tcaaggaggt ccgcgccctc accagcctgg gcctgaagga ggccaaggac ctggtggacg
     1021 gcgcccccaa gccggtgctg gagaaggtca acaaggagac cgccgacaag gccaaggagc
     1081 agctcgagtc ggcgggcgcg accgtcgagg tcaagtgaga ggtcaagcgg gaaggaacgg
     1141 ggcgggccgt ccgcccctac cccttgcgct cgcgctgctg ctaatctccc gctacgatgc
     1201 tcgtcgggca aacatcttca gctgaagatg ccgaaggggg cgcttagcct atgaaggacg
     1261 ggaggacgtt cgggcagcgt ctgcgcgaga cgcggatcca ggccgggatg tcgcagtcgg
     1321 acctcgagga gctctcgggg atcccgaagg cgcgcctgtc gcgctacgag aacgggcacg
     1381 tggctccgtc gatccagacc ctcgagcgcc tggccagggc gctgcaggtc tccgaggcct
     1441 ccctgctggg cgaccagcgg gcggtgctgg aggagttctt cgcggtcctg accgagcggg
     1501 gggtggtgat ccagagcacg gagcaggcca aggccgtggc caacgccatg gccgacctgt
     1561 tcctctcgct cgtcccgcgc gaggtgccac cgagcgtcga ggcgggcgag cccgccctcg
     1621 gtcacgagat gcccgcgccg gccctgtccg gcagcggcgg gtggctggac ggcgactgac
     1681 ccggtgagcg ctccggccga ggatctcccg gccgcggccc ctcccaccac ttcctggcgg
     1741 tcggcgctcc acgagcgcct gcgctcccgc acggcgcggg tgggcgtggt ggggctcggc
     1801 tacgtgggcc ttcccctgct cgtgtccgcc gtgcgggccg ggttcaccgg cgtggggctg
     1861 gacctggacc ccgagcgggt cgaggccgtg cgctccggcc gctcctacct gtccgacgtc
     1921 cccgatcacg tcctgggcga gctggccgac tcgctcaccc cgtccacccg gccctcggtc
     1981 ttgcgctcct gcgatgtgat cacgatctgc gtgcccaccc cgctgaagga ccacgacccg
     2041 gacctcacct tcatcgagcg ggccggccac caggtgggcc ggtacctgcg cccggggacc
     2101 ctggcgatcc tggagtcgac cacctacccc ggcaccaccg acgagatcct ccggcccctg
     2161 ctcgagcggc gggggatggt ggccggccga gacttcgccc tggcctacgc tcccgagcgc
     2221 atcgacccgg gacgcggcct ggagcacgtg cgcgccacgc ctcgggtggt gggcgggtac
     2281 acgagcgcct gcggcaagct ggcccgagcg ttctacgcca gcttcgtgga tcaggtgcac
     2341 caggtggctt ccacccgcga ggccgagatg gccaagctga tcgagaacac ctaccgccac
     2401 gtgaacatcg cgctgatgaa cgagctcgcc ttcctctccc gcgacctgga cgtggacatc
     2461 tgggaggcga tccgggccgc ggccaccaag cccttcgggt tccaggcgtt ctggcccggc
     2521 cccggggtgg gcgggcactg catcgcgatc gacccctcgt acctgtcgtg gcgggtgggg
     2581 cagcggaccg gccaccggct gaacttcgtg gagcacgccc aggaggtcaa cgcgcggatg
     2641 cccggattcg tggtgcagcg gatcggcatg gccctgaacg accggggcaa gccgctgcgc
     2701 ggggcgcggg tgctcgggat cggcgtcacc tacaagccgg acgtgagcga cctgcgcgag
     2761 tcgccctccc tggtggtgct cgagctgctg gcggcggccg gggcccgggt ctcctaccac
     2821 gaccccctcg tgccccagct cgggctgggc gggcggacct tgaggagcaa gcccctcgtg
     2881 ccccgcgtgc tgggacagca ggactgcgtg gtggtgctca cgccgcattc cgggatcgac
     2941 ttccgcgagg tggccgaccg ctcggacctg gtgttcgaca cccggggcgt gaccccgctc
     3001 ggccaccgcc gcgccgtccg cctgtaggcg cccgggcgac cgcgcgtcgg ctcgcctccg
     3061 gaatcgtgta gtatcgcact aaacgttagg cgagcggcgg ctcgatgggc aacgaaccgc
     3121 accggagccg ccggaaggag gccccaagat ggatcccgtg ttcgggagcg cgtccgagct
     3181 cgcgacgttc ggcgccgagt ccttcacccc cacggtcctg ttcgagagcg agcggatgaa
     3241 ggtcgtgctg gccgggctgg aggccgacca gcggatcgag ctgcacgccc cccaggtgga
     3301 cctggcggtg gcggtcctgg agggcctcgg cgacctgtgg gtcgacgacc ggccccgacc
     3361 cgtgcgcccc ggcgacgtgg cggtcatccc ggccggctcc acgcgggggg tccgggcccg
     3421 cgggggtcga ttggtgctcc tgcacgtggt ctccccgccg ccgagcgcag ccgaccacca
     3481 ggtggagcat cgggcctggc cctcggagga ggccggtccc gaggtccggg cggtgctgca
     3541 ccaggagcac caggagctgc tgccgcacct gggccacctc ggggcgttgg ccgagcaggc
     3601 cccggacctg gacgaggcga ccctgcgtga gcggctgcac ggcgtggtgg acttcctcac
     3661 ccactcgctc ctgccccacg cgggtgtgga ggagcagacg ctgtacccgg cggtggacgg
     3721 tctgctccgg gcgaccggcg gcgggaccgg gacgatgtcg atcgatcacg ccgagatcgg
     3781 ggagcggatc caggccctgg ccgcgatggt ggaggagccg ctcaacccct cggcgcgcac
     3841 ggcgatcgtc cgggccctgg agcagctcga ggcgatcgtg cgtctgcact tccgcaagga
     3901 ggaggaggcg tacctgcccc tgctctcgcg gctctcggcg gcggaggcct cggagctctc
     3961 ggacgcgctg tccgcgctcg gagccgagca ccatcacgag ggctgaggtc gaccgcggcc
     4021 ctcgccgtct gccgctcggg ccggcgccgg ctcacggctc gggttccagc cacaccttca
     4081 cccggctccc gctcccaggc tgggaccaga tcgacatggc cgccccgatc aggcgggcgc
     4141 gttcctccat gcccagcagt cccaggtgcc cccgccgggc gaacgcatcg gtgggtcggc
     4201 gccggaaccc gcgcccgtcg tcctccacgc tgatgctggc cgacccgtga tcgaactgca
     4261 tgtccacgaa gacccgggac gcaccggcgt gtcgctcgac gttccggagg gcctcctgcg
     4321 tgatgcggaa aagggcgacc tccacctcgg gggccagcct cctgacctgt ccatggaccc
     4381 ggaaaccggc cccgatcccc gtccggttcc ccatgtcctc caccagccac tcgagggccg
     4441 gcgcgagccc caggtcgtcc aggagggccg gccggaggtc ccgtccgaag cgccggagcc
     4501 ccgacagggc cgaccccgag cgggtccgca ggtcctccag gcgatcggcc accctccctg
     4561 gcagcccatc gccggaggcg atcagctcgt ccagctcccg gcgcatcacg accagctcct
     4621 gggccgtctc gtcgtgcagg tccctggcga tccgggctcg ctcgtcctcc tgcgccgcca
     4681 ccacctggcg gacgtaggag tgcaggcgtt cgcgctcccg ctcgtggagc cgcacgttgt
     4741 cggcgacggc cccgatctgg ctggccgcgg cggagagcag cccctcgtcc tcctcgcgga
     4801 ggccttggcc gggatccgcc gagacggcca ggcatcccac cgaggttccc tccacctgga
     4861 gggggaacac gcgcaccgca ccaccgtccc gttcgcccgg tgagcgctcc gcgctcccga
     4921 tggcctcctc gcaggcgccg aggagcgggt cccgcagctc ggaggcgggg ggcacgcata
     4981 cgcgcggccc ctcctccccg tcgggcccga ccagcagcag gcctgcaccc cgcagcccca
     5041 ccacctcgac cagccgggtc agcgctcggc tcagcccggt ccgcagctcc cgttcctgct
     5101 ccaggatcgc cgacacctcg tttaggagca ccaggcgctg gctggtggac tccgcccgga
     5161 gccgctccct gcgctcgcgc tccacggcga gcgccaccag cgtccccagg atcgcgacga
     5221 ccagcatgaa gatggcgtcc cccagccctg cgatcgacac gtggtggaag agcaggatgt
     5281 tcggcaccac gagcaccgcc gaccacaggc aggtgagcgt ggcgccttcc aacccgtact
     5341 cgaacccggc gtaggtgatg ggtaccgcgt agaacacgac cggcagatgc aggagcgcca
     5401 gggacgcggc cagctcgtga ccccagctgc tctccaccac caggtggagc agcgtcacca
     5461 ccgcgacctg cgcctggatc agccagaact gacgttcgcg ggcccgctcg ggagcggccc
     5521 ggacaaacgc gaggaggcga gccccgtacc ggatgtcacg atgcaccgag gaggtgacca
     5581 tcaggccagc tcctccaggg agaaccatcc ctcccgcaac gctcgcacca ccgcctccgt
     5641 cctggaagcg acctgtagct tggagaagat gtgggccagg tgcacctgca cggtgcgggc
     5701 gctgagatcc atgctgcggg cgatctcctt gttgctcatg ccccgcgccg ccagccgcag
     5761 gacgcccagc tcgcgatcgg tcagcgaccg tgggctccga tcggtcccct cgtcaccatc
     5821 gggttggagg cgaccgagca ccttccggac cacggtggga tgcagcaccg gctcccccac
     5881 cccgaccgcc tccacggcct cgatcaggcg ctcgctcggc acgtccttca gcaggtaccc
     5941 ggccgctccg gcctccagga cggcgaagac gtactggtcc tcatcgtgca cggtcaggac
     6001 cagcaccccg acctccggac aggtggcctt gatccggcgg acggcctcga gcccgcccat
     6061 cttgggcatg ccgagatcca ggatggccac atcgggccgg agggcgctca ccaggcggac
     6121 cgcttcctcc ccgtcggagg cctcgcccac cacctcgatg cccccgtgac gttccaggat
     6181 ctcgcgggtc ccttcccgca ccaccgtgtg atcgtccgcc aggatcaccc tcgttcccat
     6241 gatccccgca ccatacggcg ccctcaccgc ctccacgaag gggcgtccgg ccccctccac
     6301 cgtgcccgag gtcacgtcct ttataggcca gatggcctat agggccgggg ccggccgcgc
     6361 cccgggtggg acggcgtggt gggatcgcct aggcacgaat gccgacccgt cgttgcgcgt
     6421 gctgccgatg tggcgcgggg gtgctcgccc ctatggtcgt gccgatggat ctccctaccg
     6481 ggaggagcgg catgcagagt ggtgatcggc caaccggacc gcccacgacc gcgtcgggca
     6541 ccgactcggt ccagcacggc atgtccaggc gccggttcct gggctacgcc ggggcgctca
     6601 cgggtgtggc caccggaggt ctgctcccct gggcggagcg ggccgacgcg gccgaggccc
     6661 gggacacggc gatccgcgtc caccgctggg cgatggtgat cgatctgcgc aggtgcgacg
     6721 gatgctcggg gatcggcctt cctccccagt gcacccagta ctgcatctgg ggtcacctgg
     6781 ccccccaggg gatgcagtgg atcgaggtct tcgatacgac ccagagcaac ctccccggcg
     6841 ctcccggccg gttcctgccc gccccctgca tgcagtgcca gaacgcgccg tgcgccaacg
     6901 tctgtccggt gggagccacg ttccatactc ccgagggcac cgtgctgatc gatcaggagc
     6961 gctgcatcgg gtgccgcctg tgcatggcgg cctgccccta cgaccggcgc ttcttcaact
     7021 ggggagagcc cgtccagccc gagtacgcca ccgatcagcc ctacgacgtg ctggatcagg
     7081 tcccggcgat caagggcacc gtgatgaagt gcgatttctg caccgacctc gccgaggcgg
     7141 gggggatctc gttctgcgcg gatgcctgcc cccagggagc gatctactac ggcgatttgg
     7201 aggaggacgt ggccaccaac ggggtcgact tcgtccggct ctcgaccttc atcgaggaga
     7261 accacgccac gccctacaag gaggagctgg gcaccgagcc ccgggtcttc tacatcgccg
     7321 gcgacggcga gctggccagc gagagctcgg ccgaccccag ggaccgggag ttcctgaagg
     7381 gcaacctgga gtggccgtgg gcccggatcc agcaggcgct cgagggccag gcaccggtcg
     7441 gagcctcctc cacccaggga ggcagcgatg cttgatccgg ccgccgacca cctggagcgc
     7501 gtggggctcg cgcccctccg gcgcaccacc tggcggtact ggctgctcct cgcgttcctg
     7561 gccgcggtgg ccgggctggg gttctacgcc tacgccgacc agctctccaa gggcctgtcc
     7621 gtcaccggga tgcgcgtctc cctgaacaag atcatctggg gcctgtacat caccaacttc
     7681 gtgttcttca tcggcatcag ccacgccggc accctcatct cggcgatcct ccgggtcagc
     7741 aaggcggggt ggcgcacccc catcaccagg atggccgagt tcatcacggt ggtggcgctc
     7801 ctggtcggtg gcctgttccc cctgatcgac atgggacgcc cggaccgggt cctgaacctg
     7861 cttcggttcg ggcggtggca gtcgccgatc ctgtgggacg tcctggccat cgccacctac
     7921 ctgaccggca gcctgatcta cctgtacctg cccctgatcc ccgatctggc cacctgccgc
     7981 gacctgatcg gcggcgg
//
LOCUS       DASQQQ010000224         4114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_183575, whole genome shotgun sequence.
ACCESSION   DASQQQ010000224 DASQQQ010000000
VERSION     DASQQQ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183575"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..479)
                     /locus_tag="VE646_08550"
     CDS             complement(75..479)
                     /locus_tag="VE646_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80076.1"
                     /translation="MSRVRMLFLAVALTGCHVGKGVEDFAPATRPEGIEARVVTRNGG
                     RIVGELLAVEDSGLLIRTVKSFVFVPYGQIRQGRFPVSGAPGALDDGRRPGNELERWL
                     RLLSRYPQGVDGDLLERLLAAYGFEEVERLDR"
     gene            complement(487..2121)
                     /locus_tag="VE646_08555"
     CDS             complement(487..2121)
                     /locus_tag="VE646_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80077.1"
                     /translation="MRADSSKHPLPAHARAGRRAALRIAAGRLLALAAATGLAACVVI
                     FRHPDVAPVETTADSVAIRSPVKAHRVDGATVIYEDGVVLVRDTLRGNGVSYDLRLSP
                     SRSVPLLPVDSVVALESYQTRVNAAASIIVSTILTAPLAAGVALAIACATNPKCFGSC
                     PTYYSREGDRFVLEAEGFSYSIAPLLEARDVDRLGARPSPDGVLRLEVRNEALETHYH
                     NHMELLAVAHAPDERVLPDPRGEPIAVRGALAPVTAVDRAGRDLGPVVAVADGHAFES
                     DRRLLGEAATGELRDFIELSVPRPEGVDSVALILRLRNSLLATVLLYDVMLGDPGPRA
                     VDWVGEDLQRIDRAVKLGRWYVEYFGLGIDVDEGGTYRRAAWFRDTGPIAWKELAVPI
                     PVPAGAGDSLRVRLSFVTDNWRIDRLAVAAAFRRPGARGLPVSRVIGNDGRENAGALE
                     ALRAPDARYLQVYPGGRFTVEFDAGSVEPGVARTFMVASQGYYIEWIRRAWLRSAGAD
                     RDFEPSERAVREAIARWASGRQELEARFAATRIPVR"
     gene            2742..3071
                     /locus_tag="VE646_08560"
     CDS             2742..3071
                     /locus_tag="VE646_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZD80078.1"
                     /translation="MTVNALFTVVAEPNRRRILDLLRDRERPVGELVQALDVAQPTVS
                     KHLKVLREVGLVEFRVEGRRRVYTLRAEPLFAVDAWLAPYRVRWARRLEALERHLETM
                     PDEGDPA"
     gene            3068..3601
                     /locus_tag="VE646_08565"
     CDS             3068..3601
                     /locus_tag="VE646_08565"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZD80079.1"
                     /translation="MKYGTLEPAGDRWRITYERRLAHPPEKVWRALTEAEHLAAWFPS
                     TIEGDWEVGAPLRFVFPFEEAPVLEGTVLAYEPPRLLELTWGEDHLRFELTPEGGGCH
                     LRFAVTIVELGKGARDGAGWHACLDALARHLDGDAPAAVRAAREGWKEVHPAYVKSFG
                     PEASVIGPPDWHPETGG"
     gene            3805..4080
                     /locus_tag="VE646_08570"
     CDS             3805..4080
                     /locus_tag="VE646_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80080.1"
                     /translation="MIVAIPEALRDRLGPEAARALAELLNEVSVRTRGDVIEMAAGRF
                     ERRLAEEIGKVRAEIGASEGRITRWMFVFWIGQIGALLGILFAFFRS"
BASE COUNT          612 a         1466 c         1455 g          581 t
ORIGIN      
        1 gaccgcggtt tcggcggcga agcccacgct caacgtcgcc cagcccaggg ccagcagggt
       61 cagcgctccg ttcctcatcg gtcgagccgc tccacttcct cgaagccgta ggcggcgagg
      121 agccgctcca acagatcgcc gtccacgccc tgggggtagc gggagagcag ccgaagccac
      181 cgctccagct cgttacccgg ccggcgcccg tcgtcgaggg cgcccggcgc acccgacacg
      241 gggaacctgc cctgtcggat ctggccgtag ggcacgaaca cgaagctttt gaccgtgcgg
      301 atgagcagcc cgctgtcctc gacggccagc agctcgccca cgatcctccc cccgttccga
      361 gtcaccaccc gggcctcgat cccctccggc ctcgtggcgg gagcgaagtc ctcgacgccc
      421 ttgcccacat ggcagcccgt cagggccact gccaggaaca gcatccggac gcgcgacatg
      481 gctggctcac ctcacgggga tgcgggtggc ggcgaatcgg gcctccagct cctgccggcc
      541 cgaagcccag cgtgcgatcg cctcgcggac cgctcgctcg gacggctcga agtctcgatc
      601 ggcgcccgcc gagcgcagcc aggcccgacg gatccactcg atgtaatagc cctgcgacgc
      661 caccatgaag gtacgggcga cgcccggctc cacggagccc gcgtcgaact cgaccgtgaa
      721 gcgcccgccc ggatacacct gcaggtagcg ggcgtccggg gcacgcagcg cttccagggc
      781 cccggcgttc tcccggccgt cgttgccgat cacacgggag acgggcagcc cgcgtgcccc
      841 gggtctgcgg aaagcagcgg ccaccgccag ccgatcgatc cgccagttgt cggtgacgaa
      901 cgagagccgc actcgcagcg agtcgccggc gcccgccggc accgggatcg ggaccgccag
      961 ctccttccac gcgatcgggc cggtgtcccg gaaccacgcg gcacgccggt acgtcccgcc
     1021 ctcgtccacg tcgatcccca gaccgaaata ctctacatac cagcggccca gcttcaccgc
     1081 tcgatcgatc cgttgaagat cctcgcccac ccagtccacc gcccgcgggc cgggatcgcc
     1141 cagcatcacg tcgtacagca gcacggtggc cagcaggctg ttgcgcagcc gcaggatcag
     1201 cgccacgctg tccacgcctt ccggcctcgg gacgctcagc tcgatgaagt cgcgcagctc
     1261 gccggtggcc gcttcgccca gcagacggcg gtcgctctcg aaggcgtgac cgtcggccac
     1321 ggccacgacg ggccccaggt cacgaccggc acggtcgacg gcggtgacgg gagccagcgc
     1381 gccgcgcacg gcgatcggct ctccgcgtgg gtccggcagc acccgctcgt cgggcgcatg
     1441 ggcgacggcg aggagctcca tgtggttgtg gtagtgggtc tccagtgcct cgttccgaac
     1501 ctccagccgc agcacgccgt cgggcgacgg ccgcgcaccc agccggtcca cgtcacgggc
     1561 ctccaggagc ggcgcgatgc tgtacgagaa gccctccgcc tccaggacga accggtcgcc
     1621 ctcccggctg tagtaggtag gacaggagcc gaagcacttg ggattcgtgg cgcaggcgat
     1681 ggcgagcgcg acgccggcgg cgagcggcgc cgtgaggatc gtgcttacta tgatgctcgc
     1741 cgcggcgttc acccgcgtct ggtagctctc cagagccacc acgctgtcca ccggcagcag
     1801 cggcaccgac cgggacgggc tcagccggag gtcgtaggag acaccgttcc cgcggagcgt
     1861 gtcccggacc agtaccacgc cgtcctcgta gatcaccgtg gcaccgtcca cccgatgggc
     1921 cttcaccggg ctgcggatcg ccaccgagtc ggccgttgtc tctacgggcg ccacgtccgg
     1981 gtgccggaag atcaccacgc acgcggcgag ccccgtggcg gccgccaggg cgaggagccg
     2041 tcccgcggcg atcctgaggg cggcacgacg cccggcgcgg gcgtgggcag gtagggggtg
     2101 cttcgaggag tcggctcgca tcggttctcg ctccgcaggg ccctggtggg ccgtggacct
     2161 ccagtcgcgg ggccgacgat cgacgccgcg agtcaagcgc gagtcaaggc gagacgggtc
     2221 gagggtttgg agcggaccga gcctccgggc cgaggcggag gagggacgcc agggtgctga
     2281 cggaggcccg ggctgggcca ggcgggccca tggggcaggc ccgaagggac ccgcggtcgc
     2341 ctgacggctc ggagcccctc gctgcggagt ccggcctgcg ggtgagacgc gcgccggtcg
     2401 ccgcaccact ctgtcagcga gagcggccgg ccccgtcccg gctcacccgg ccccagcgtg
     2461 cgacgtatgt cagcgacgcg gatcaccttc tatcgccgcc tcagggtcgc ggagtactgg
     2521 atcgtggacc gggatgctga gctcgtggag gtctgccggc tcgagcttct gatctggcct
     2581 cctccgggcc cgactccgcc tcaccgtaca ataatctccc gattcgaggc gtcggcgcag
     2641 gctggccccc agcatgcatg cgacgcatca acagatcctc aaccgggtgt gtcgggcggg
     2701 ccatcatgtt ccttgacaga aatataactg tgcggtaata tatgaccgtg aacgcgctct
     2761 tcaccgtcgt cgccgagccc aatcgccgcc gcatcctgga tctcctcagg gaccgggagc
     2821 gcccggtcgg ggagctggtc caggccctcg acgtggccca gcccaccgtc tccaagcacc
     2881 tcaaggtgct cagggaggtc gggctggtcg aattccgcgt cgagggccgg cggcgcgtct
     2941 acacgctccg agccgaaccc ctcttcgccg tggacgcctg gctggcgccg taccgggtcc
     3001 ggtgggcccg gcgcctggag gccctggagc gccatctcga gaccatgccc gatgaaggag
     3061 accccgcatg aagtacggaa ccctcgagcc ggcaggggac cgctggcgca tcacgtacga
     3121 gcgcaggctc gcccacccgc ccgagaaggt ctggagggcc ctcaccgagg cggagcacct
     3181 ggcggcgtgg ttcccgtcga cgatcgaggg cgactgggag gtcggcgctc cgctgcgctt
     3241 cgtgtttccc ttcgaggagg cgcccgtcct ggaggggacc gtgctcgcgt acgagccgcc
     3301 gcggctgttg gagctgacct ggggagagga ccatttgcgc ttcgagctca cgccggaggg
     3361 cggcggctgt cacctccgct tcgcggtgac gatcgtggag ctggggaagg gcgcccggga
     3421 cggggccggg tggcacgcct gtctcgacgc tctggcccgc cacctggacg gcgacgcgcc
     3481 cgccgcggtg cgggccgccc gggaggggtg gaaagaggtg catccggcgt acgtgaagag
     3541 cttcggcccc gaggcgtcgg tcatcggtcc gccggactgg caccccgaga ccggaggctg
     3601 acgcttcgac gcagggccgc ggggcgggga gggactacag cgtaacccta ttctgccgag
     3661 cgcccggatc gtctcgcacg agacgagcgg gagtaccgtc atctgtagat gacgccgcgt
     3721 tgacaggcca cccgcagctt cctcccgacc gttgctcgcc cggaggcggc gagcgtacgt
     3781 tacgcatgga ggtgtaggca gggcttgatc gtcgcgatcc cggaggcgtt gcgggacagg
     3841 ctggggcccg aggcggcccg agcgttggcg gagctgctca acgaggtgag cgtgaggacc
     3901 cgaggcgacg tgatcgagat ggccgccggc cggttcgagc gccggctggc cgaggagatc
     3961 ggcaaggtcc gcgccgagat cggcgcgtcg gaggggcgga tcacccgctg gatgttcgtc
     4021 ttctggatcg ggcagatcgg ggcgttgctc gggatcctct tcgctttctt ccgctcctga
     4081 caccgaggcc tgcctgcagc ggctctattt cctt
//
LOCUS       DASQQQ010000225        14381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_400133, whole genome shotgun sequence.
ACCESSION   DASQQQ010000225 DASQQQ010000000
VERSION     DASQQQ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14381
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400133"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VE646_08575"
     CDS             complement(<1..977)
                     /locus_tag="VE646_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HZD80081.1"
                     /translation="MPPFKVRAEFELSGDQPEAVDRLVEGIRAGEDRITLLGATGTGK
                     TFTIAHAIERIQKPTLVMAPNKSLAAQLANEFREFFPENAVEYFVSYYDYYQPEAYVP
                     QTDTYIEKDSSINDEIERLRHSATAALLTRPDVIIVASVSAIYGLGTPEEYMGQVLRL
                     HQHVELDRDFAIQRLVDIQYDRNQVNLARGRFRVQGDVLEVHPAYEEIAIRVEWWGDE
                     VERITRFDPLTGEVLEELGEVWIYPATHYVASHERMQRAIAGIEQELQQRLAYLDSHS
                     KLLEAERLRMRTQYDLEMLREVGFCSGIENYSRHIDGRVRGEAPYTLLD"
     gene            complement(1025..1543)
                     /locus_tag="VE646_08580"
     CDS             complement(1025..1543)
                     /locus_tag="VE646_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZD80082.1"
                     /translation="MAEPDLVTRRGAIDILERGRVAIAGLLGRVPAAELDHPGVGGGE
                     WSPKDLVGHLASWEERALEALAAWGRGEPAPIDREFRSRTLTAINAEAVAAKAGLSYA
                     EVRREARRTHAELLRAIEAIPDGRWGSPATPRGRRPLGHRLGQILVGTGPFAHAQAHV
                     ESLRAFVDRFDS"
     gene            complement(1543..2307)
                     /locus_tag="VE646_08585"
     CDS             complement(1543..2307)
                     /locus_tag="VE646_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZD80083.1"
                     /translation="MRPDGADGRVLRVEGLHKSFGKLEVLKGIDLSVSEHEVVCLIGA
                     SGSGKSTLLRCIDLLEPIDHGRIVIHGQEVTDRGVDVNSVRRHIGIVFQSFNLFPHMS
                     VLRNITLGPTEALKLSRSDAQTRAVELLERFGLAEKKEDYPDRLSGGQQQRVAIIRSL
                     AMQPDILLLDEVTSALDPELVSGVLDLIRELAAGGMTMVIATHEMGFARDTAHRVVFL
                     HEGVILEEGTPEQIFTQPREPRTQRFLQSIIEAGRM"
     gene            complement(2304..3110)
                     /locus_tag="VE646_08590"
     CDS             complement(2304..3110)
                     /locus_tag="VE646_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZD80084.1"
                     /translation="MRQEGAKGVLIAVVSTIVVFAILVVIVVRSPNWPTVRQAFFNGQ
                     EFRDTFPTIASRFVRNVEYFMIAEMFILGWALILAVMRSLPGPVFFPLRLMSIAYTDL
                     FRGIPTILVVYLLGFGVPAMQLPGVPSSPTFWAVIALILSYSAYVAEVYRAGIESIHP
                     SQVASARSLGLTRVQSLRFVVVPQAVRRVIPPLLNDFVGLQKDTALVAFVVAIPEAFK
                     QAQLAQYADFNFTPYLAAALMFLIVTIPMTRLVDWLIARERARRQAGAAL"
     gene            complement(3219..4130)
                     /locus_tag="VE646_08595"
     CDS             complement(3219..4130)
                     /locus_tag="VE646_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZD80085.1"
                     /translation="MRRMLVAVAAFGLLLAACASNENPVVAPSAGGGGSPSSSATAAD
                     CAQGVTTYVNSGQLTVGTDNPAYPPWYAGDDTQGTNWKINNPATGKGFESAVAYAVAD
                     KMGFSRDQVQWVEVPFNQSFAPGPKSFDFDINQISYSPKRAKAVDFSQSYYDVNPALV
                     ALQGTPIAKATSLSDLKNYSLASQIGTTDYDYIANVIQPTKEPGAYNSLADAVQALKS
                     GNVDGIVVDLPTAFYITAVQIPQGVIVGQFPAEGQQEYFAMAFQKGNSLVQCVNQALD
                     ELKSEGTLKAIQDRWLSSKVSVPVLST"
     gene            complement(4232..5020)
                     /locus_tag="VE646_08600"
     CDS             complement(4232..5020)
                     /locus_tag="VE646_08600"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD80086.1"
                     /translation="MIDARLALRQARFTNKAFWRNPASAFFTFAFPLLFLVIFTSFLG
                     NGTALFVLPSGARFRLEQSTYFVAAMAAFSVITACYTNIAMGVTYQRDVGILKRTRGT
                     PLPGWAYMTGRVLQALGVGFLLVVITAAFGIAFYGAHVPTGIDLVRFLVVLVVGAMSF
                     AALGLATTAAVPNADAAPAVVNGIILPLLFVSGIFFPIDATAPQWIRVLGGIFPVKHF
                     ADAMLAGFYGAPLPFHWRDVLILGAWGVAGLMLAVRFFSWEPRK"
     gene            complement(5017..5925)
                     /locus_tag="VE646_08605"
     CDS             complement(5017..5925)
                     /locus_tag="VE646_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD80087.1"
                     /translation="MAASAIEVRGLRKSYGELEAVRGIDFEVGAGEVFALLGPNGAGK
                     TTTVEILEGYRARTAGEVRVLGHDPAERDRAFRQRVGIVLQSTGIDPYLTVRETVQLY
                     AGYYPNPRNVDEVLEVVGLTEKLDTRVNRLSGGQQRRLDVAIGLAGDPELLFLDEPTT
                     GFDPGARRNAWEVVRNLTALGKTVLLTTHYMDEAQTLAHRVAVLSKGQIVAEGPPGTL
                     AGRNEVHARIRYRLPEGSPELPEWGQLHLGNGTYEMVVEDPTKMLHEVTGWALDRGVR
                     FETIEVVRPSLEDVYLELTSPEVEPA"
     gene            complement(5955..6932)
                     /locus_tag="VE646_08610"
     CDS             complement(5955..6932)
                     /locus_tag="VE646_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014912812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HZD80088.1"
                     /translation="MSDRPGIVVLGAEASDPPPGLDGVEEVADLTYASTLPELEGALP
                     GTEVIFAWHAARGVLPAAWPHATDLKWIQTASAGVDWLLFPELVASHVVVTNARGVFD
                     EAMAEYVLGLMLAFAKGLPATLEHQRRKEWRHRDAEMLDGRRLLVAGVGPIGRAIGRL
                     AMVLGMDVRGIGRTARIGDDVFETIHGSDELAEAVGWADWIVNALPLTEDTGGLFDAA
                     VFARMAPKARFVNVGRGATVDQSALIEALMEGRIAGAALDVFEQEPLAQDSPLWVLPN
                     VLISPHMSGNFAGWRESSVEVFLENLVRYVSGRPLTNVVNKERGYPTGP"
     gene            complement(6929..7306)
                     /locus_tag="VE646_08615"
     CDS             complement(6929..7306)
                     /locus_tag="VE646_08615"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HZD80089.1"
                     /translation="MTERTGRVYTPEEADATLPELRALLERVRANRQVILREVERIKA
                     TVARDGGGHGGKGYWEASAALKADVQSLAERDILLRDPETGLVDFPAERDGRPIFLCW
                     RMGEERVGHWHEIHSGFVGRQPL"
     gene            complement(7303..8007)
                     /locus_tag="VE646_08620"
     CDS             complement(7303..8007)
                     /locus_tag="VE646_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HZD80090.1"
                     /translation="MRYEAVFFDAGETLVHPHPTFPDLFATILRREGFDVSAQTVHDR
                     IHVVFDRFRQAAETNELWTTTPEKSKRFWHDVYHIFFRELGIPDTDELIGAVYGEFTD
                     TANYALFEDVVPALQLLRSHGLRLGVISNFEEWLERLLDQLGVSVFFPVRVISGVEGL
                     EKPDLRIFELALERAGASAARSVYVGDNPDLDLEPAAAVGMLPVLIDRRERFPQRDGC
                     RITTLAELPTVLELSV"
     gene            complement(8035..8652)
                     /gene="coaE"
                     /locus_tag="VE646_08625"
     CDS             complement(8035..8652)
                     /gene="coaE"
                     /locus_tag="VE646_08625"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HZD80091.1"
                     /translation="MLLVGLTGGIGSGKSTVARLLEQRGAIVFDADVLARAAVEPGAL
                     GHRQVVERFGAQVLAPGGGIDREALASIVFRDPAARRDLEAIVHPEVRRLFAEGCERF
                     DGTDRVVVFSAPLLVETGMHSAFDVLLVVSTSVQTQVDRLMRERGMPEAQARDRIAAQ
                     APVEVKAELADHLIENEGSMADLEVQVERVWTDLVERAHAGAASG"
     gene            complement(8654..9976)
                     /gene="rpsA"
                     /locus_tag="VE646_08630"
     CDS             complement(8654..9976)
                     /gene="rpsA"
                     /locus_tag="VE646_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HZD80092.1"
                     /translation="MEASLRDFKDGDIVDGEVVKIDRDEVLVDIGYKSEGVIPTKELS
                     IRHDVDPNEVVKVGDQIEALVLQKEDKEGRLILSKKRAQYERAWGRIEEVMQSGGTIK
                     GPVIEVVKGGLILDIGLRGFLPASLVDLRRVRDLHPFVGTELEAKIIELDRNRNNVVL
                     SRRAFLEESQSEDRKKFLESLKKGERRKGRVSSIVNFGAFVDLGGVDGLVHVSELSWK
                     HVDHPSEVVQVGQEVEVEVLDVDLERERVSLSLKATQEDPWKEFERKYQAGEIIEGQV
                     TKLVPFGAFVRVAQGIEGLVHISEISEAHVDTPESVLSVGDQVRVKVIEVDVPRRRIS
                     LSMRQVGDALPTIKEPEIQEEPEPEPQTAPLATAEPALQPEPAPVAVAPAEAAPAPTE
                     PSPAAQEPEAPGPPEPVVPEQVAEAEEQEEVSLESILEDLKRREGRTD"
     gene            complement(10192..12831)
                     /gene="polA"
                     /locus_tag="VE646_08635"
     CDS             complement(10192..12831)
                     /gene="polA"
                     /locus_tag="VE646_08635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HZD80093.1"
                     /translation="MATDTPARRLFLLDGHSLSYRAFFALPQSLATSTGQVTNAVYGF
                     TSMLIKLLAEEEPDLIAVAFDIGKPTVRLEKYAEYKAGRPETPDEFRQQLGLIVEVLE
                     TLHIPVIGVEGHEADDAIATLATRASRLGIETTIVTADRDFFQLVRPGIRVMFNVKGI
                     SEIKEYDERAVEERFGLPPSRYLDYVALKGDPSDNIPGVPGVGEKTATRLIQQYGSVE
                     ELLAHTEELKGKLKASIEEVGEQLALNKELALLQTDLGLDVGPDDCVMGEWDMDSVHR
                     LFTSLEFRSLLERLEEVARGAKPAVEVAELDLRQAPERELVQLLGSDGPKGVRIELDV
                     RTATGIAVSAGGGQAAYAPLPTLQQVGPLLADPEVPKWMHDAKEAESAVLATGGTLRG
                     LRFDTMLAGYLLDPATASYPLEGLCEQYLGTDVLGAVGEESEGQLFGQSWRRAAAEAA
                     AVALLAPVMAERIAKAGLGPLLEEVELPLSSVLARMQAAGVALDVEYLHEMASGVTDR
                     MGELRDAIYRDAGEEFNLNSPPQLRTILYEKLGLSPGKRTPKGQLSTDASVLEKLRDQ
                     HPIVDALLQWRELDKLNSTYLEALPKQVDPADGRVHTTFNQTGAATGRLSSVNPNLQN
                     IPVRGELGRQIRRAFIPGSDGQFLLVADYSQIELRILAHLSEDEGLRAAFDSGTDIHT
                     ATSARVFGLPEEQVDAELRRRAKAINYGLAYGMNAYGLASRLEIAPDEAQEFIDAYFA
                     SFPRIKEYLERQVARATADGFTETILGRRRYLPELQASNPRVRDMGRRMALNAPIQGS
                     AADVFKLGMIKVDRALETSALSCRMLLTVHDELVFEVAEDQVEAAARLVGQQMGSAYE
                     LSVPLRADIGWGRNWAEAAPAGH"
     gene            12985..13800
                     /locus_tag="VE646_08640"
     CDS             12985..13800
                     /locus_tag="VE646_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HZD80094.1"
                     /translation="MSTTQTVLLGAIAGFTIFLGLPMGRMQSQSARLRTFLNGVSAGI
                     LVFLLFDILEHATEPVEEAVADGAWGRLVAVGVLYLGGLALGLIGLFYVGRLWRRRGA
                     PAGVGPGAMAVAEADAVRSDALRVGMAIATGIGLHNFSEGLAIGQAAHSGSVSLALLL
                     VIGFALHNGTEGFGIVGPLAATGQRASWGWLAAAGLIGGGPTFLGTLVGTAVSSDLLF
                     VGFLALAAGAILYVIGELFGAGRRLSWEVTLWGVFAGFGLGLLTELVLVAAGA"
     gene            14193..>14381
                     /locus_tag="VE646_08645"
     CDS             14193..>14381
                     /locus_tag="VE646_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80095.1"
                     /translation="MKAMVRPRDARLDVLRKVPLLSGLSKREVARVLAVSTEEEFFPG
                     VVIVEAGNRASDFYLVLQG"
BASE COUNT         2153 a         5075 c         4874 g         2279 t
ORIGIN      
        1 tagtcgagca gggtgtacgg cgcctcgcca cgcacgcgcc cgtcgatgtg gcgggagtag
       61 ttctcgatac cgctgcagaa ccccacctca cgcagcatct ccaggtcgta ctgggtgcgc
      121 atccgcaggc gctcggcctc gagcagcttg ctgtgcgagt cgaggtacgc gagccgctgc
      181 tgcagctcct gctcgatgcc ggcgatcgcc cgctgcatcc gctcgtggga ggccacgtag
      241 tgcgtggcgg ggtagatcca cacctccccg agctcctcca ggacctcgcc ggtcagcgga
      301 tcgaagcggg tgatgcgctc gacctcatcc ccccaccact cgacgcggat ggcgatctcc
      361 tcgtaggccg ggtgcacctc gagaacgtcg ccctggaccc ggaaccgacc ccgggccagg
      421 ttcacctggt tgcggtcgta ctggatgtcg accagccgct ggatcgcgaa gtcgcggtcc
      481 agctccacgt gctggtggag ccgcaggacc tgccccatgt actcctcggg ggtgcccagg
      541 ccgtagatcg ccgagacgct ggccacgatg atcacgtcgg gccgggtgag gagcgccgcg
      601 gtggccgagt ggcgcagccg ttcgatctca tcgttgatcg agctgtcctt ctcgatgtac
      661 gtatcggtct gcgggacgta ggcctcgggc tggtagtagt cgtagtagct cacgaagtac
      721 tcgaccgcgt tctcggggaa gaactcccgg aactcgttgg ccagctgcgc ggccagggac
      781 ttgttcgggg ccatcaccag cgtgggcttc tggatgcgct cgatggcgtg ggcgatcgtg
      841 aacgtctttc cggttccggt ggccccgagg agggtgatcc ggtcctcccc ggcccggatg
      901 ccctcgacca gccgatccac ggcctcgggc tggtcaccgg agagctcgaa ctcggcacgg
      961 accttgaagg ggggcataga ccgattctag ggccgaagcc cgacacgtcc cgccggggac
     1021 aggttcacga gtcgaatcga tccacgaacg cgcgcaggct ctccacgtgc gcctgcgcgt
     1081 gcgcgaaggg accggtgccg accaggatct ggcccaggcg atgtcccagg ggcctgcggc
     1141 cccggggcgt ggccggggaa ccccaccgcc cgtcggggat cgcctcgatc gcccggagca
     1201 gctcggcgtg cgtccggcgc gcctccctgc gcacctccgc atagctcagt cccgccttgg
     1261 ccgccaccgc ctccgcattg atcgcggtga gggtccggct tcggaactcg cggtcgatcg
     1321 gggccggctc cccgcggccc catgccgcga gcgcctccaa ggctcgctcc tcccagcttg
     1381 ccaggtggcc caccaggtcc ttgggggacc actcccctcc tccaacgccc gggtgatcca
     1441 gctcggccgc cgggacacgg ccgagcagtc cggcgatggc cacacgtcct cgctcgagga
     1501 tgtcgatggc cccacgcctg gtgaccaggt ccggctcggc catcacatcc tcccggcctc
     1561 gatgatgctc tgcaggaacc gctgggtgcg cggctcccga ggctgggtga agatctgctc
     1621 cggcgtgccc tcctccaaga tcacgccctc gtggaggaac accacccggt gggccgtgtc
     1681 gcgcgcgaac cccatctcgt gggtggcgat caccatggtc atgccgccgg ccgccagctc
     1741 gcggatcagg tccaggaccc cggagaccag ctccgggtcc agtgcgctgg tcacctcgtc
     1801 gagcaggagg atgtcgggct gcatggccag cgagcggatg atcgccaccc gctgctgctg
     1861 tccgcccgag agccggtccg ggtagtcctc cttcttctcc gcgagcccga agcgctccaa
     1921 aagctccacg gcgcgtgtct gcgcgtcgct cctggagagc ttcagcgcct cggtcggccc
     1981 cagcgtgatg ttgcggagca cggacatgtg cgggaagagg ttgaacgact ggaacacgat
     2041 gccgatgtgc cggcgaacgc tgttcacgtc cacaccgcgg tcggtgacct cctgtccgtg
     2101 gatcacgatc cggccgtgat cgatcggctc gagcaggtcg atgcaccgga gcagggtgga
     2161 cttccccgac cccgaggccc cgatcagaca gaccacttcg tgctcggaca cggagagatc
     2221 gatccccttc agcacctcga gctttccgaa ggacttgtgg agcccctcga cccggagcac
     2281 ccgcccgtcg gcgccgtcgg gcctcacagg gcggctccgg cctggcggcg ggcacgctcc
     2341 ctggcgatca gccaatccac gagccgcgtc atggggatcg tcacgatcag gaacatcagg
     2401 gctgccgcca ggtagggcgt gaagttgaag tcagcgtact gggccagctg cgcctgcttg
     2461 aaggcctccg ggatggccac gacgaaggcc accagcgcag tgtccttctg caggccgacg
     2521 aagtcgttca ggagcggcgg gatcacgcga cggaccgcct gaggcaccac cacgaagcgc
     2581 agcgactgga ccctggtgag acccagggac cgggccgagg ccacctggct gggatggatc
     2641 gactcgatcc ccgcccggta gacctcggcc acgtaggccg agtagctgag gatcagcgcg
     2701 atcaccgccc agaaggtcgg cgagctgggg acccccggca gctgcattgc cggtaccccg
     2761 aagcccagca ggtacacgac caggatcgtg gggatccctc ggaacagatc ggtgtaggcg
     2821 atcgacatca gccgcagcgg gaagaacacc ggtccgggca ggctgcgcat cacggccagg
     2881 atcagcgccc agcccaggat gaacatctcc gcgatcatga agtattcaac gttgcggacg
     2941 aaccggctcg cgatcgtggg gaaggtgtct cggaactcct gaccattgaa gaaggcctgc
     3001 cgcaccgtcg gccagttcgg agagcgaacc acgatcacca cgaggatcgc gaacaccacg
     3061 atagtgctga ccacggcgat caggacgccc ttggctccct cctgccgcac gccggcgagc
     3121 aggcgcgaac gacctccccg ctcggcgtga tgacgcccgg gggcctcatc gaggtccgga
     3181 ggtccccctc cgccgcccca ggacatcgtt ccaggcggtt acgtcgacaa gaccggcacg
     3241 ctgaccttgc tcgacagcca ccgatcctgg atcgccttca gcgttccttc ggacttcagc
     3301 tcgtcgagcg cttggttcac gcactggacc agagagttgc ccttctggaa cgccatggcg
     3361 aagtattcct gctgcccctc ggccgggaac tgcccgacga tcactccctg cgggatctgc
     3421 acggccgtga tgtagaaggc ggtgggcagg tcaaccacga tcccgtcgac attgcccgac
     3481 ttcagcgcct ggaccgcgtc cgcgaggctg ttgtaggcac cgggctcctt ggtgggctgg
     3541 atcacgttcg cgatgtagtc gtagtcggtg gtcccgatct gcgacgccag ggagtagttc
     3601 ttgaggtccg acaggctggt ggccttcgcg atcggcgtgc cctgcaaggc gacgagcgcg
     3661 ggattcacgt cgtagtagct ctggctgaag tccaccgcct tggcccgctt gggcgagtac
     3721 gagatctggt tgatgtcgaa gtcgaagctc ttggggccgg gcgcgaagga ctggttgaac
     3781 ggcacctcca cccattgcac ctggtcccgg gagaagccca tcttgtccgc taccgcgtag
     3841 gcgaccgcgc tctcgaagcc cttgcccgtg gccgggttgt tgatcttcca gttcgtgccc
     3901 tgggtgtcgt cgcccgcgta ccacggcggg taagccggat tgtcggttcc cacggtcagc
     3961 tgcccggagt tcacgtaggt ggtgacgcct tgcgcgcagt cggcggccgt ggccgaggag
     4021 ctcggcgagc ctccgccccc cgcactgggc gcgaccaccg ggttctcgtt gctcgcacat
     4081 gccgccagca gtagcccgaa cgctgccacc gcgaccagca tcctgcgcat gatgaccccc
     4141 ctgggttcct gatcggcccc gcgaccgtgc gcgagcctag cacacgccat ctcctacgaa
     4201 cgtgccggtg gggcgggttg ttccgggacg actacttcct gggctcccag ctgaagaacc
     4261 gcaccgccag catcaggccg gcgacccccc acgctcccag gatcaacacg tcgcgccagt
     4321 ggaacggcag tggtgcgccg tagaacccgg ccagcatggc atcggcgaaa tgcttcacgg
     4381 ggaagatgcc gcccagcacc ctgatccact gcggagccgt ggcatcgatg gggaagaaga
     4441 tccccgagac gaacagcagc ggcaagatga tcccgttcac caccgcgggc gcggcgtcgg
     4501 cgttcgggac ggcggccgtc gtggccagcc cgagcgcggc gaagctcatc gcgcccacca
     4561 ccaggaccac caggaaccgc accaggtcga tgcccgtggg gacgtgggcg ccgtagaagg
     4621 cgatcccgaa cgccgccgtg atcaccacga gcaggaaccc cacgccgagg gcctgcagca
     4681 cccgcccggt catgtaggcc catcccggga gtggggtgcc ccgggtccgc ttcaggatcc
     4741 ccacgtcgcg ctggtacgtc acgcccatcg cgatgttcgt gtagcaggcc gtgatcaccg
     4801 agaacgccgc catcgcggct acgaagtacg tggactgctc gagccggaac ctggcccctg
     4861 aggggagcac gaagagggcc gtcccgtttc ccaggaagga ggtgaagatg accaggaaca
     4921 gcagggggaa ggcgaaggtg aagaaggccg acgccgggtt gcgccagaag gccttgttcg
     4981 tgaaccgggc ctgccgcagc gccagtcgag catcgatcac gccggctcga cctcggggct
     5041 ggtgagctcc aagtagacgt cctccaggct ggggcggacc acctcgatcg tctcgaaccg
     5101 gaccccccgg tccagggccc acccggtgac ctcgtgcagc atcttcgtgg ggtcctccac
     5161 caccatctcg taggtcccgt tgcccaggtg gagttggccc cactccggca gctccggaga
     5221 gccctcgggc aggcggtagc ggatccgagc gtgcacctcg ttgcgtccgg cgagcgtccc
     5281 gggcggtcct tcggccacga tctgcccctt cgacagcacc gccacccgat gggccagggt
     5341 ctgggcctcg tccatgtagt gggtggtcag cagcacggtc ttgcccagcg ccgtcaggtt
     5401 ccgcaccacc tcccaggcgt tgcgtctggc gcccgggtcg aacccggtgg tgggctcgtc
     5461 caggaacagc agctcggggt ccccggcgag cccgatggcc acgtccaggc gccgctgctg
     5521 gccccccgag agccggttca cgcgcgtatc gagcttctcg gtgagcccga ccacctccag
     5581 gacctcatcg acgttgcggg ggttcgggta gtagccggcg tacagctgca ccgtctcgcg
     5641 gaccgtgagg tacgggtcga tcccggtgga ctgcaggacg atccccaccc gctggcggaa
     5701 ggcgcggtcg cgctcggccg gatcgtggcc cagcacgcgc acctcgccgg ccgtgcgagc
     5761 ccgatagccc tccaggatct ccacggtggt ggtcttgccg gcgccgttgg ggcccagcag
     5821 ggcgaagacc tcgcccgcgc cgacctcgaa gtcgatgccc ctcacggcct ccagctcgcc
     5881 gtaggacttg cgcaggccac ggacctcgat cgcggatgca gccatgtgaa cggacagtct
     5941 acgtggccgg tccgctatgg accggtgggg tacccccgct ccttgttcac cacgttggtg
     6001 agcggccggc cgctcacgta ccggaccagg ttctccagga agacctcgac cgaggattcg
     6061 cgccatcccg cgaagttgcc cgacatgtgc ggggagatca gcacgttcgg gagcacccac
     6121 agaggactgt cctgggccaa cggctcctgc tcgaacacgt cgagcgccgc gcccgcgatc
     6181 cggccctcca tcagagcctc gatcaatgcg ctctggtcca cggtggcgcc tcgaccgacg
     6241 ttcacgaacc gagctttcgg ggccatccgc gcgaacacgg cggcgtcgaa cagcccgccg
     6301 gtgtcctcgg tgagcgggag ggcgttcacg atccagtccg cccagcccac ggcctcggcc
     6361 agttcgtctg agccgtggat cgtctcgaac acgtcgtcgc cgatccgagc cgtacgccct
     6421 atcccgcgca cgtccatccc caggaccatg gccaaacgtc cgatggcccg gccgatcggc
     6481 cccacccccg ccaccagcag gcggcggccg tcgagcatct cggcgtcgcg gtgccgccac
     6541 tccttgcggc gctggtgctc cagcgtggcg ggtaggccct tcgcgaacgc cagcatcagc
     6601 ccgagcacgt attcggccat ggcctcgtcg aacacccctc gggcgttggt gaccaccacg
     6661 tggctggcca ccagctccgg gaagagcagc cagtccacac cggccgaggc cgtctggatc
     6721 cacttcaggt ccgtggcatg gggccaggcg gcgggcagga ccccccgcgc cgcgtgccag
     6781 gcgaagatca cttccgtgcc cgggagggcg ccctccagct ccggcagcgt ggaagcgtag
     6841 gtgagatcgg ccacctcctc cacgccgtcc aggccgggcg gcgggtccga cgcctccgcc
     6901 cccagcacca cgatcccggg gcgatcgctc acaggggctg tcgtcccacg aaacccgagt
     6961 ggatctcgtg ccagtggccc acccgctcct cccccatccg ccagcacagg aagatgggtc
     7021 ggccgtcgcg ctcggccggg aagtcgacca gcccggtctc cggatcgcgg agcaggatgt
     7081 cccgttccgc cagggactgc acgtcggcct tcagggcggc ggacgcctcc cagtaccctt
     7141 tgccgccgtg accgccaccg tcccgagcca cggtggcctt gatccgctcc acctcccgga
     7201 ggatcacctg ccggttggcc cgtacccgct ccaggagcgc acgcagctcg ggcagggtgg
     7261 cgtcggcctc ctccggtgtg tagacacggc cggtgcgctc ggtcacacgc tcagctccag
     7321 gacggtgggg agctcggcca gcgtggtgat gcggcaccca tcgcgctgcg ggaagcgctc
     7381 gcgacgatcg atcagcaccg ggagcatgcc caccgccgcg gccggctcga ggtccaggtc
     7441 cgggttgtcg cccacgtaga cggaccgcgc cgccgacgcc ccggcccgct ccaaggccag
     7501 ctcgaagatc cggaggtcag gcttctccag cccctccacg cccgagatca cccggaccgg
     7561 gaagaacacg gagactccca actggtcgag gagccgctcc agccactcct cgaagttcga
     7621 gatgaccccc agccggaggc cgtgcgaccg caggagctgc agcgccggaa ccacgtcctc
     7681 gaacagggcg tagttcgccg tgtcggtgaa ctcgccgtac acggctccga tcagctcgtc
     7741 ggtatcgggg atcccgagct cgcggaagaa gatgtggtag acgtcgtgcc agaaccgctt
     7801 ggacttctcg ggtgtggtgg tccatagctc gttcgtctcc gccgcctgcc ggaagcggtc
     7861 gaagaccacg tggatccggt cgtgcaccgt ctgcgcgctc acgtcgaagc cctcgcggcg
     7921 gaggatcgtg gcgaacaggt ccgggaaggt ggggtgcgga tgcaccaggg tctcccccgc
     7981 gtcgaagaag accgcctcgt acctcacggc gccgatgata gcgaccggga ggcctcagcc
     8041 ggacgccgct ccagcgtgcg cccgctcgac caggtccgtc cacacccgct cgacctgcac
     8101 ctccaggtcg gccatgctgc cctcgttctc gatcaggtga tcggcgagct cggccttgac
     8161 ctccaccggc gcctgggccg cgatccgatc acgggcctgc gcctcgggca tgccgcgctc
     8221 gcgcatcagg cggtcgacct gcgtctgcac cgaggtcgaa accaccagca ggacgtcgaa
     8281 ggccgagtgc atgccggtct cgaccagcag gggcgcgctg aacaccacca cccggtcggt
     8341 cccatcgaag cgctcgcacc cctcggcgaa cagccgtcgt acctctggat ggacgatggc
     8401 ttccagatcc cgacgggcgg ccgggtcgcg gaagacgatg ctggccaggg cctcccggtc
     8461 gatcccgccc ccgggcgcca ggacctgggc gccgaaccgc tccaccacct gccggtggcc
     8521 cagcgccccc ggctccaccg cggctcgagc cagcacgtcg gcatcgaaca cgatcgcccc
     8581 tcgctgctcg aggagccgag ccaccgtgga cttgcccgat ccgatcccgc cggtgaggcc
     8641 gacgaggagc acctcagtcc gtgcggcctt cccgccgctt gaggtcctcg aggatcgact
     8701 cgagggagac ctcttcttgc tcctcagcct cggccacctg ctcgggcacg acgggttcgg
     8761 gcggacccgg agcctcgggc tcctgggcgg ccggtgacgg ctccgtgggg gcgggtgcgg
     8821 cctcggccgg cgcgacggcc accggagcgg gctccggctg gagtgcgggc tcggcggtgg
     8881 cgagcggggc cgtctgtggc tccggctcgg gctcctcctg gatctcgggc tccttgatgg
     8941 tgggcagcgc gtctcccacc tggcgcatcg acaggctgat ccgccgcctg ggcacgtcca
     9001 cctcgatcac cttcacccgg acctggtccc cgaccgacag gaccgactcg ggggtgtcca
     9061 cgtgcgcctc ggagatctcg gagatgtgga ccagaccctc gatcccctgc gccacccgga
     9121 cgaaggcacc gaacggcacg agcttggtga cctggccctc gatgatctcg ccggcctggt
     9181 acttgcgctc gaactccttc cagggatcct cctgcgtggc tttgagcgag agcgacacgc
     9241 gctcgcgctc caggtccacg tcgaggacct cgacctcgac ctcctgaccc acctggacga
     9301 cctcactggg gtggtcgacg tgcttccagg acagctccga gacgtggacc agaccgtcca
     9361 cgccgccgag atccacgaac gcgccgaagt tcacgatgga agagaccctg cccttgcgcc
     9421 gctcgccctt cttcagcgac tccaggaact tcttccggtc ctcgctctgg gactcctcca
     9481 ggaaggcccg gcgggacagg acgacgttgt tacggttgcg gtccagctcg atgatcttcg
     9541 cctcgagctc ggtccccacg aacgggtgca ggtcgcggac ccggcgcaga tccaccaggg
     9601 aggcggggag gaacccgcgg aggccgatat cgaggatcag cccgcccttg acgacctcga
     9661 tcaccgggcc cttgatggtg ccgccggact gcatgacctc ctcgatccgg ccccaggcgc
     9721 gttcgtactg ggcgcgcttc ttggacagga tcaggcggcc ctccttgtcc tccttctgga
     9781 gcaccagggc ctcgatctgg tcgccgacct tgaccacctc gttggggtcg acgtcgtggc
     9841 ggatcgacag ctccttggtg gggatgaccc cctcggactt gtagccgatg tcgaccagca
     9901 cctcgtcgcg gtcgatcttc acgacctcgc cgtcgacgat gtcgccgtcc ttgaaatcgc
     9961 ggagggaggc ctccatggcc tcgcggagct cctccggcgt caggtcgcgc cccggcgcgg
    10021 ccttggtctc gctcacgtct atctgaacca tctccttctc cgcgcgcagg gcgcgcaccg
    10081 cggcgagtgg gaattgccga ggtgcggatg cgcctcgcgg ctcgggaatg gtagccgcga
    10141 ccgatttcgc acgtcaacga ccgaacgcgg agctggcgct gccggaccgg ctcagtggcc
    10201 ggcgggggcg gcctcggccc agttccgccc ccagccgatg tccgctcgca gcggcacgct
    10261 gagttcgtac gccgagccca tctgctgccc caccaggcgg gcggccgcct cgacctgatc
    10321 ctccgccacc tcgaagacca gctcgtcgtg aacggtgagc agcatccgac acgacagggc
    10381 ggaggtctcg agggcccggt ccaccttgat catgcccagc ttgaacacgt cggccgcgct
    10441 gccctggatc ggggcattca gcgccatccg gcggcccatg tcgcggaccc gggggttcga
    10501 ggcctggagc tccggcaggt accgccggcg gcccaggatg gtctcggtga agccgtcggc
    10561 ggtggccctg gccacctggc gctccaggta ctccttgatg cgggggaagc tggcgaagta
    10621 ggcgtcgatg aactcctggg cctcgtccgg ggcgatctcc agccgcgagg cgagcccgta
    10681 ggcgttcatg ccgtaggcca gtccgtagtt gatggccttg gcccggcgtc ggagctcggc
    10741 gtccacctgc tcctcgggca gaccgaacac gcgagccgag gtcgccgtgt ggatgtcggt
    10801 cccggagtcg aaggcggccc gcagcccctc gtcctcggac aggtgcgcca ggatccggag
    10861 ctcgatctgc gagtagtcgg cgaccagcag gaactggccg tcggagcccg ggatgaacgc
    10921 ccggcggatc tgccgtccca gctcgccccg gaccgggatg ttctgcaggt tggggttcac
    10981 cgaggacagc cgcccggtgg ctgcgccggt ctggttgaag gtggtgtgga ccctcccatc
    11041 cgccgggtcg acctgcttgg gcagcgcctc caggtaggtg gagttcagct tgtccagctc
    11101 gcgccactgg agcagcgcgt ccacgatggg gtgctggtcc cggagcttct ccagcacgct
    11161 ggcgtcggtg gagagctgcc ccttgggggt tcgtttgccg ggggacagcc cgagcttctc
    11221 gtacaggatc gtccgcagct ggggcgggga gttcaggttg aactcctccc cggcgtcccg
    11281 gtagatggca tcccggagct cccccatgcg atcggtgacg ccggaggcca tctcgtgcag
    11341 atactccacg tccagggcca cgccggcggc ctgcatccgg gccagcaccg aggacagcgg
    11401 cagctccacc tcctccagga gcggaccgag gccggccttc gcgatccgct cggccatgac
    11461 cggagccagc agagccaccg ccgcggcttc ggccgccgcg cgccgccagc tctgaccgaa
    11521 cagctgtccc tcggactcct cccccaccgc gcccagcacg tcggtcccca ggtactgctc
    11581 gcacagcccc tccagcggat aggaggcggt ggccgggtcg agcaggtacc ccgccagcat
    11641 cgtgtcgaag cgcagccccc ggagcgtccc gccggtggcg agcacggccg actcggcctc
    11701 cttggcgtcg tgcatccact tgggcacctc gggatcggcc agcaacgggc ccacctgctg
    11761 gagcgtgggc agcggggcgt aggcggcctg gcctccgccc gctgagacgg cgatcccggt
    11821 cgcggtccgg acgtcgagct cgatccggac ccccttcggg ccgtccgagc ccaggagctg
    11881 caccaactcc cgctccgggg cctgccgcag gtcgagttcc gccacctcca cggccggctt
    11941 ggccccgcgg gccacctcct ccaggcgctc cagcagcgag cggaactcga gcgaggtgaa
    12001 cagccggtgc acgctgtcca tgtcccactc gcccatcacg cagtcgtcgg ggccgacgtc
    12061 cagccccaga tcggtctgga gcagggccag ctccttgttc agcgcgagct gttcgcccac
    12121 ctcctcgatc gacgccttga gcttgccctt cagctcctcg gtgtgcgcca gcagctcctc
    12181 caccgatccg tattgctgga tcaggcgggt ggccgtcttc tcccccacac ccgggacgcc
    12241 cgggatgttg tcggaggggt cgcccttcag agccacgtag tcgaggtacc gggagggcgg
    12301 caggccgaag cgctcctcca ccgcgcgctc gtcgtactcc ttgatctccg agatgccctt
    12361 gacgttgaac atcacccgga tcccagggcg cacgagctgg aagaagtccc ggtcggccgt
    12421 gacgatggtg gtctcgatcc cgagccggct ggcccgcgtg gccaaggtgg cgatggcgtc
    12481 gtcggcttca tgcccctcca ccccgatcac ggggatgtgc agtgtctcca ggacctcgac
    12541 gatcaggccg agctgctggc ggaactcgtc gggcgtctcg ggacggcccg ccttgtactc
    12601 ggcgtacttc tcgagccgaa cggtgggctt gccgatgtcg aacgccaccg cgatgagatc
    12661 gggctcctcc tcggccagga gcttgatcag catcgaggtg aagccgtaca cggcgttggt
    12721 gacctggccc gtgctggtgg ccaggctctg ggggagggcg aagaaggccc ggtaggacag
    12781 gctgtgcccg tccagcagga agagccgtct cgcgggggtg tcggtggcca cctgcggatt
    12841 ctagtgcccc gcccgacgtg ctccggaccg acgtctggac cggggaccga gcggatagct
    12901 atcgttcggt cccgaacgag gttgcgatga atccgacctt gtgggtagga ttcggccgat
    12961 ccgagccgct gcatcggagt ggtgatgagc acgacacaga ccgttctgct gggcgccatc
    13021 gcggggttca cgatcttcct ggggctgccc atggggcgga tgcagagcca gagcgctcgc
    13081 ctacgaacct tcctgaacgg cgtgtccgcc gggatcctgg tcttcctgct gttcgacatc
    13141 ctcgagcacg ccaccgagcc cgtggaggaa gcggtggcgg acggcgcgtg gggtcgcctg
    13201 gtggcggtcg gggtcttgta cctgggcggg ctggccctcg gcctgatcgg gctgttctac
    13261 gtggggcgcc tgtggcggcg tcggggggct ccggccgggg tgggaccggg cgcgatggcg
    13321 gtggccgagg ccgatgccgt ccgctccgac gccctgcgcg tggggatggc catcgccacc
    13381 gggatcggcc tgcacaactt ctccgagggc ctcgcgatcg gacaggccgc gcactcgggc
    13441 tcggtctcgc tggcgctcct gctggtgatc ggtttcgccc tccacaacgg caccgagggg
    13501 ttcggcatcg tgggaccgct cgcagccacc ggccagcgag cctcgtgggg atggctcgcc
    13561 gcggccggcc tgatcggcgg cggcccgacc ttcctgggga cgctggtggg gaccgccgtc
    13621 agttcggacc tgcttttcgt gggcttcctg gcgctcgcgg ccggcgcgat cctgtacgtg
    13681 atcggtgagt tgttcggcgc gggtcgacgc ctctcgtggg aggtcacgct gtggggcgtg
    13741 ttcgcggggt tcgggctcgg gctgctgacc gagctagtgt tggtcgccgc cggcgcctga
    13801 ggatcgggtc cccaccgggc agaggaatgt cactctttct tcggtctccg ctgcgtggcc
    13861 gctacctggg ctgtttcctc gaagttcctg caccgcaagg gctggcgcgt gttgacaggg
    13921 gtggggcgcc tcgctagatt tcgggccggt cccaagcggc cgctgggatc gggaggcggg
    13981 aagcgcctcc gagccggacc ggaggggatg tgcagggcgc gagccccgaa ccccccacca
    14041 gccggagcgg agggcgcacc ggccgaccgg ataccgggtc gcttggggcc aactcttggc
    14101 gggtcgggga accgagtaga taacgctcgc gtatggggaa ggcgcgagcg gctccaaagg
    14161 ttcctcgacc cgccgtacct ttgctgccgg gggtgaaggc gatggtccga ccgagggatg
    14221 cgaggttgga tgtgcttcgc aaggtccctc tcctcagtgg actttccaag agggaagtcg
    14281 ccagggtcct cgctgtgagc accgaggagg aattcttccc gggcgtcgtc atcgtggaag
    14341 cgggcaaccg ggcgagcgac ttctacctgg tgctgcaggg g
//
LOCUS       DASQQQ010000226         6881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_314673, whole genome shotgun sequence.
ACCESSION   DASQQQ010000226 DASQQQ010000000
VERSION     DASQQQ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314673"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VE646_08650"
     CDS             <1..535
                     /locus_tag="VE646_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80096.1"
                     /translation="HALLVLGVVAGVAVVAVLLMNVLGGGGGGDNVAQPATPIATGSP
                     VLSPSISPTPTPRETLPPVVLAGSRNPFSIPPPLQSPSGSVGPSSTVTPSISPTETPT
                     TPGGGQSEEIGAHTLVLLDVFGGGTKAQVEVDGKVYTVAEGQTFDGNFKLVSISGQCA
                     RFLYGDEPFSLCTNPQK"
     gene            897..2048
                     /gene="aroC"
                     /locus_tag="VE646_08655"
     CDS             897..2048
                     /gene="aroC"
                     /locus_tag="VE646_08655"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HZD80097.1"
                     /translation="MLRLLTAGESHGQALTGMLEGLPAGVPIAPKEIAGELARRRHGY
                     GRSARQKLEADSFQILSGVRHGRTLGTPISITIGNAEWGPKYQDLMGVEGRVDPGDRL
                     TRPRPGHADLVGVQKYGFDDVRNVLERASARETVARVAVGTFCKAFLAELGIQLLSHV
                     VRIGRVAVPATARPPGPEDLEAVDASPVRALEARISERMVAEIDTAMHERDTLGGVFE
                     VLAYGCPPGLGSHVHYDRKLDARLALVLMSIQSVKGVEVGDGFATAARRGSKAHDEIV
                     RTGARLSRKTGRAGGIEGGMSTGEPIRVRAAMKPISTVPRALATVDLATGAADVAIKQ
                     RTDICAVPAGGVVGEAAVAFVLAQAVLEKFGGDSLAETRRNLDAYLAEL"
     gene            2074..2574
                     /locus_tag="VE646_08660"
     CDS             2074..2574
                     /locus_tag="VE646_08660"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HZD80098.1"
                     /translation="MIVYLVGMPGSGKSTVGPELARLLEVPFVELDSQIEREAGRTVR
                     EIFAEQGETRFRELEAAALTEAATNDPSVVSCGGGVVLEPANRVTLRATGEVVFLWVP
                     LEVLRERVRPAEDRPLIHAQEDLERLFREREPLYREFAVHEVDASGPPQEVAAAIRKE
                     LTRWSA"
     gene            2562..3650
                     /gene="aroB"
                     /locus_tag="VE646_08665"
     CDS             2562..3650
                     /gene="aroB"
                     /locus_tag="VE646_08665"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HZD80099.1"
                     /translation="MVSLRIAVPGRPYEAVIGRGLLRGAGAHLPQLPRAERAFVVADH
                     AVAERYLEPLAAGLGARGLEVVPLGVPQGEEAKSLSTWMALHHQLAVQEAHRDDLVVA
                     LGGGSVGDLAGFVAATYMRGVPFVQVPTTLTAQVDASIGGKTAVNLPEGKNLVGAFYQ
                     PRAVLADVDTLATLAEAEFRSGLGEVAKVGLTLDLELLELLEQRPEGILAREPALLED
                     VVARCVGAKARVVAADERDAGERLVLNYGHTLGHALERLDAFGGRSHGEAVAVGMVFA
                     ARLAESLGWAPAGLAARTTRLLKPLGLEPDGRLPAADRILGAMRMDKKYRGGIRFVVL
                     EEPGRTRVVEGVPADAVREVLCEMGAAG"
     gene            3647..4081
                     /locus_tag="VE646_08670"
     CDS             3647..4081
                     /locus_tag="VE646_08670"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HZD80100.1"
                     /translation="MRVLYLFGPNLGALGTRDPDRYGDRTLEQVMTEVAERASALGHR
                     VEWRQSDHEGDLVGWLNDARARGVEAAVLNPGALTHYSYALRDAVEACGVPVVEVHQT
                     NIYAREDFRRHSVISPVARASIAGAGAGGYHLALEALSWITP"
     gene            4066..5139
                     /locus_tag="VE646_08675"
     CDS             4066..5139
                     /locus_tag="VE646_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019047839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HZD80101.1"
                     /translation="MDHALRRGRLAEWLQQAGIEALLVNRLPNVRYLTGFTGSNGQVI
                     VRADGRDLFLTDGRYEEQARHEVPDLERRGYRESMVRAVRDIVPALGLGRLGFEVDGL
                     TYHAYTELGADLPAELVGVNGAVERLRRVKDADELRILDRAQEVTDAAFEALLPKLRE
                     GVSERELAFELEIELRTAGADDLSFETIAAFGEDAAEPHHDPGGRPLRRGDVVKLDFG
                     ALVDGYHCDMTRTVAFGHPPRELHEIHDLVRRAQRAGIEAVRAGVDAAGVDAAARSVI
                     EEAGMGERFAHGLGHGVGLEIHEDPFLHRSSQEVLPAGAVVTIEPGVYVPGLGGVRIE
                     DMVEVREDGRRALPRSSKELIVT"
     gene            5136..5699
                     /gene="efp"
                     /locus_tag="VE646_08680"
     CDS             5136..5699
                     /gene="efp"
                     /locus_tag="VE646_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012903488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZD80102.1"
                     /translation="MSVSTNDLKNGMTLDLDGTLFQVIEFQHVKPGKGGAFVRTKLRN
                     VRTGQVLERTFNAGVKVDQAIVERKDMQYLYRDGDNLVFMDTGTYEQLMVPVDVVGDA
                     ASYLAEAATAQVSVHEGVPIGVELPASVVLSITDTQPAVRGDTRTSAMKPATLETGYE
                     VQVPLFVEPGEKVKVDTRSGEYIERVK"
     gene            5708..6109
                     /gene="nusB"
                     /locus_tag="VE646_08685"
     CDS             5708..6109
                     /gene="nusB"
                     /locus_tag="VE646_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZD80103.1"
                     /translation="MASRHEARRQALDVLYQADVTGADPLEVLGEWRSAGRKVSRFAR
                     ELVEGVAANLGELDGVIGEHAEGWTVPRLAAVDRTILRVGVYELHHVAETPPAVAIDE
                     AVEAAKELSTEDSGRFVNGILGRIVREGAEP"
     gene            complement(6128..6391)
                     /gene="tatA"
                     /locus_tag="VE646_08690"
     CDS             complement(6128..6391)
                     /gene="tatA"
                     /locus_tag="VE646_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016570840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZD80104.1"
                     /translation="MPDIGIPELLIILAVVVLLFGAKKLPELARSLGRSASEFKAGVQ
                     EGGAEQKATGETGGAAGTPSGGSATAQTNGQVAETKPADTQGE"
     gene            complement(6517..6642)
                     /gene="tatA"
                     /locus_tag="VE646_08695"
                     /pseudo
     CDS             complement(6517..6642)
                     /gene="tatA"
                     /locus_tag="VE646_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014514826.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
BASE COUNT          947 a         2419 c         2547 g          968 t
ORIGIN      
        1 ccatgcgctg ctcgtcctcg gcgtggtcgc cggggtggcg gtcgtcgctg tcctgttgat
       61 gaacgtgctg ggaggggggg gcgggggcga caacgtcgcc cagcccgcga cgcccatcgc
      121 caccgggagc ccggtgctga gtccgtcgat ctcgcctacc cccacgcccc gcgagacgct
      181 gccgcccgtg gtcctggccg ggtcgcggaa cccgttctcg atcccgcccc cgctccagtc
      241 cccgagcggc agcgtcggcc cctcctcaac cgtgaccccg agcatctcgc ccaccgagac
      301 gccgaccact ccaggcggtg gccagagcga ggagatcggc gcgcacaccc tggtcctgct
      361 ggacgtgttc ggcggcggga cgaaggctca ggtggaggtc gacggcaagg tctacacggt
      421 cgccgagggt caaaccttcg acgggaactt caagctcgtc tcgatcagcg ggcagtgcgc
      481 ccggttcctg tacggcgacg agcccttcag cctctgcacg aacccgcaga agtagatgca
      541 ggagtggcag gactccggcg agccgcgcac ggggggagcg gtcgccggcg acgagagggc
      601 ggtccgggtg atccggaccg ccctccacca cgtccggggt gcatccagcc cctgaactct
      661 tggcggtccc ggtcaagatc ctgatccccc agccctcctc atctcctgcg acccgtcacg
      721 atcactcaca acgaacgccg gcggtggatg ctcacagggc cctgacgaga acggcgcgaa
      781 gaacggcccg gtccgcgtcg accggccgac gcgggggccg tgcccacccc gcccccgtca
      841 ccgccgcacc acgcccctca ccgggccgtc cccacccgtc acctatgatg tcgcccatgc
      901 tgcggctgct cacggccggt gagtcgcacg gccaggccct gaccggaatg ctcgaggggc
      961 tgcccgccgg ggtccccatc gcgcccaagg agatcgccgg cgagctcgcc cgccgtcggc
     1021 acggctacgg ccgcagcgcc cgtcagaagc tagaggccga cagcttccag atcctctccg
     1081 gggtccgcca cgggcgcacc ctgggcaccc ccatctcgat caccatcggc aacgcggagt
     1141 gggggcccaa gtaccaggac ctgatgggcg tcgagggccg ggtcgacccg ggggaccgcc
     1201 tcacccgccc ccgtcccggg cacgccgacc tggtgggcgt gcagaagtac ggcttcgacg
     1261 acgtccgcaa cgtcctggag cgggccagtg cccgggagac cgtggcccgg gtggccgtgg
     1321 gcaccttctg caaggccttc ctggccgagc tcgggatcca actgctgtcc cacgtggtgc
     1381 gcatcgggcg ggtggccgtg cccgccaccg ctcgcccgcc cgggcccgag gatctggagg
     1441 ccgtcgacgc ctcgccggtg cgggcgctcg aggctcggat ctcggagcgc atggtggccg
     1501 agatcgacac cgcgatgcac gagcgcgaca cgctcggcgg cgtgttcgag gtcctcgcgt
     1561 acggctgccc cccgggcctc ggctcccacg tccactacga ccgcaagctc gacgcccgct
     1621 tggccctggt cctgatgagc atccagtcgg tgaagggcgt ggaggtgggc gacggcttcg
     1681 ccaccgccgc ccgacgaggc tcgaaggcgc acgacgagat cgtccgcacg ggcgccaggc
     1741 tctcccgcaa gaccgggcgg gccggcggca tcgagggcgg gatgagcacc ggcgagccca
     1801 tccgggtccg ggcggcgatg aagccgatct ccacggtccc cagggccctg gccaccgtgg
     1861 acctggccac cggggctgcc gacgtcgcca tcaagcagcg caccgacatc tgcgccgtgc
     1921 cggccggcgg cgtggtgggg gaggcggccg tggccttcgt cctggcccag gcggtcttgg
     1981 agaagttcgg cggcgactcg ctcgccgaga cccgccgcaa cctggacgcc tacctggcgg
     2041 agctgtgatg ggccgaccgg cggggacgct cccgtgatcg tctacctggt ggggatgccg
     2101 ggctcgggca agtccacggt gggcccggag ctggcccgtc tgctggaggt gccattcgtg
     2161 gaactcgatt cgcagatcga gcgcgaggcg ggccgtacgg tccgcgagat cttcgccgag
     2221 cagggtgaga cgcgcttccg cgagctcgag gccgcggccc tcaccgaggc agcaacgaac
     2281 gacccttcgg tggtctcctg cggcggcggg gtcgtgctcg agcccgccaa ccgggtcacg
     2341 ctccgcgcca ccggcgaggt ggtgttcctc tgggtcccgc tggaggtcct gcgcgagcgg
     2401 gtccgacccg ccgaggaccg ccccctgatc cacgcccagg aggacctgga gcggctgttc
     2461 cgggagcgcg agcccctgta ccgggagttc gcggtgcacg aggtggacgc ctcgggacca
     2521 cctcaggagg tcgccgccgc gatccgcaag gagctcaccc ggtggtcagc ctgaggatcg
     2581 ccgtccccgg gcgtccctac gaggccgtga tcggccgggg tctgttgcgg ggggccgggg
     2641 cccacctgcc ccagctcccg agggccgagc gtgccttcgt ggtggccgat cacgccgtgg
     2701 ccgagcggta cctcgagccg ctcgctgccg ggctcggagc ccgagggctc gaggtggtgc
     2761 cgctgggcgt gccccagggc gaggaggcca agtccctgtc cacctggatg gcgctgcacc
     2821 atcagctcgc cgtgcaggag gcgcaccgcg acgacctggt ggtggcgctc gggggtggct
     2881 cggtgggcga tctggccggg ttcgtggccg ccacgtacat gcggggcgtg cccttcgtcc
     2941 aggtgccgac cacgctcacc gctcaggtcg acgcctcgat cggcggcaag accgcggtca
     3001 acctgccgga gggcaagaac ctggtgggcg ccttctacca gccccgcgcc gtgctggccg
     3061 acgtggacac gttggccacg cttgccgagg ccgagttccg ctcgggcctg ggcgaggtgg
     3121 ccaaggtagg cctgaccctc gacctggagc tgctcgagct cctcgagcag cggcccgagg
     3181 gcatcctggc ccgcgagccc gccctcctgg aggacgtcgt ggcccggtgc gtgggggcca
     3241 aggcccgggt ggtggccgcc gacgagcgcg acgcgggcga gcgtctggtc ctgaactacg
     3301 gacataccct gggccacgct ctggagcggc tcgatgcctt cggggggcgc tcgcacggcg
     3361 aagccgtcgc ggtggggatg gtgttcgcgg cccgcctggc cgagtcgctc ggatgggcgc
     3421 cggccggtct ggccgcccgg accacccggc tgctgaagcc gctcggcctg gagcccgacg
     3481 ggcgcctgcc ggccgccgac cggatcctcg gcgcgatgcg gatggacaag aagtaccggg
     3541 gcgggatccg gttcgtcgtg ctggaggagc cgggtcggac tcgggtggtg gagggcgtcc
     3601 cggccgatgc cgtccgcgag gtcctgtgcg agatgggggc ggccgggtga gggtgctgta
     3661 cctgttcggg ccgaacctcg gcgccctcgg cacccgggat cccgaccggt acggcgaccg
     3721 caccctcgaa caggtcatga ccgaggtcgc cgagcgggcc agcgccctcg gccaccgggt
     3781 ggagtggcgg cagtccgacc acgaggggga cctggtgggc tggctgaacg acgcccgggc
     3841 ccggggcgtc gaagcggcgg tcctgaaccc cggggcgctc acgcactact cgtacgccct
     3901 gcgcgatgcc gtggaggcct gtggggtccc ggtggtggag gtgcaccaga cgaacatcta
     3961 cgcgcgcgag gacttccgcc ggcactcggt gatctcgccg gtggcgcgcg cgagcatcgc
     4021 cggagcgggt gccggcgggt accacctggc gctggaggcc ctgtcgtgga tcacgccctg
     4081 aggcgcggcc gcctggccga gtggctccaa caggccggga tcgaggccct gctggtgaac
     4141 cgtctcccca acgtccgcta cctcaccggg ttcaccggct ccaacgggca ggtgatcgtc
     4201 cgcgccgacg ggcgggacct gttcctcacc gacgggcggt acgaggagca ggcccgccac
     4261 gaggtgccgg acctcgagcg gcgcggctac cgcgagagca tggtccgcgc ggtccgcgac
     4321 atcgtgcccg cgctcggcct gggccgcctg ggcttcgagg tcgatggcct cacgtaccac
     4381 gcctacacgg agctgggtgc ggacctgccg gccgagctgg tcggtgtgaa cggcgcggtg
     4441 gagcggctgc ggcgggtcaa ggacgcggac gagctccgga tcctcgaccg agcccaggag
     4501 gtcaccgacg cggccttcga ggccctcctg cccaagctgc gcgagggcgt gagcgagcgc
     4561 gagctggcct tcgagctcga gatcgagctg cgcacggccg gcgccgacga cctgtcgttc
     4621 gagaccatcg cggcgttcgg ggaggacgcc gccgagcccc accacgaccc aggggggcgg
     4681 ccgctccgcc ggggtgacgt ggtgaagctg gacttcggcg cgctggtgga cggctaccac
     4741 tgcgacatga cccgcacggt ggcgttcggc catcccccgc gggagctgca cgagatccac
     4801 gacctggtcc gccgggccca gcgggccggg atcgaggcgg tgcgggccgg ggtggacgcg
     4861 gccggcgtgg acgcggccgc gcgctcggtg atcgaggagg ccgggatggg ggagcgcttc
     4921 gcccacggcc tcggtcatgg ggtggggctc gagatccacg aggatccctt cctgcaccgg
     4981 agctcgcagg aggtcctgcc ggccggcgcg gtggtcacga tcgagccggg cgtgtacgtt
     5041 ccgggcctgg gcggcgtccg catcgaggac atggtcgagg tcagggagga cggccgccgg
     5101 gcgctgcctc gatcgagcaa ggagctgatc gtcacgtgag cgtgtccacc aacgacctga
     5161 agaacgggat gaccctggac ctggacggca cgttattcca ggtgatcgag ttccagcacg
     5221 tgaagcccgg caagggcggc gccttcgtgc ggaccaagct ccggaacgtg cggaccgggc
     5281 aggtgctgga gcgcaccttc aacgccgggg tgaaggtgga tcaggcgatc gtcgagcgta
     5341 aggacatgca gtacctgtac cgggacggcg acaacctggt gttcatggac acgggcacct
     5401 acgagcagct gatggtgccg gtcgacgtgg tgggcgatgc cgccagctac ctggccgagg
     5461 ccgccaccgc tcaggtctcg gtgcacgagg gggtcccgat cggcgtggag ctgccggcct
     5521 cggtggtgct gagcatcacg gacacgcagc ccgcggtgag gggcgacacc cggaccagcg
     5581 cgatgaagcc ggccacgctg gagacgggct acgaggtgca ggtgccgctg ttcgtggagc
     5641 ccggcgagaa ggtcaaggtg gacacgcgct cgggcgagta catcgagcgg gtgaagtagg
     5701 gatcgccgtg gcctcgaggc acgaggcccg ccgccaggcc ctcgacgtcc tctatcaggc
     5761 cgacgtcacg ggcgccgatc cactggaggt gctgggcgag tggcgctcgg ccggccgtaa
     5821 ggtgtcgcgg ttcgctcggg agctggtgga aggggtcgcc gcgaacctgg gcgagctcga
     5881 tggcgtgatc ggggagcacg cggaaggctg gaccgtgccc cggctggcgg cggtggaccg
     5941 cacgatcctt cgggtcggcg tgtacgagct gcaccacgtc gccgagaccc ccccggcggt
     6001 ggccatcgac gaggccgtgg aggcggccaa ggagctgtcc accgaggact cgggtcggtt
     6061 cgtgaacggg atcctgggcc ggatcgtccg cgagggcgcc gagccctgac cggcctgggc
     6121 cgggcggcta ctcgccctgg gtgtcggcgg gcttggtctc ggcgacctgg ccgttcgtct
     6181 gggcggtcgc cgatccaccg ctcggggtcc cggccgcgcc accggtctca ccggtcgcct
     6241 tctgctcggc cccgccctcc tggacgccgg ccttgaactc gctggccgac cggcccaggg
     6301 agcgcgccag ctccgggagc ttcttcgccc cgaacagcag caccaccacg gccaggatga
     6361 tcaagagctc ggggatgccg atatctggca ttcgctatcg cctctttccg gaacgctcga
     6421 cggcccgagc cacccctact cgccgccgct gccgctggta tccggggtct ccgccttggg
     6481 cgagctcggc ataccggtct gtccctcgga tccaccctcc tcgagtcccc tcttgaactc
     6541 ggaggaggag cgaccgagcg aacgcgccag ctcggggagc ttcttggccc cgaacagcag
     6601 caccaccacg cccagtacga tgagcagttc aggcacaccc atcggccttc caccttcctg
     6661 caggtcggac ctctagtgta ggcccaaccg gggccctgcg tccggttcgg ggcacgagtg
     6721 atcgttcggc ccgctagttg actcacgtca agatgtcctc ggcgccacct ccggttgcct
     6781 caccaaagat gtcctcgaaa tctccgtcca ggagaacttc ctcccggcgg aggggtgtcc
     6841 gggtcgtctc acctccttgc tccgatcggg tttggtccgg t
//
LOCUS       DASQQQ010000227        30882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_314766, whole genome shotgun sequence.
ACCESSION   DASQQQ010000227 DASQQQ010000000
VERSION     DASQQQ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 30882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30882
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314766"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VE646_08700"
     CDS             complement(<1..268)
                     /locus_tag="VE646_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80105.1"
                     /translation="MKTEQTGAGTTPPPGARARATGLLERYELLSERIPFRAKLIAVW
                     IAIFAVMGGVLVAFELNVAWMRHNFWFITRGLWVTVFIAVCAIFF"
     gene            complement(286..1215)
                     /locus_tag="VE646_08705"
     CDS             complement(286..1215)
                     /locus_tag="VE646_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZD80106.1"
                     /translation="MRRSRFLIPVVLTASLTLVAAACSSGSSNDNNAAGGGGDTASPA
                     AQGTCASVDTSSGDLLARICQNGEVRVSTDPAYPPQSSYDEATGQWEGFDIDAAEEIA
                     KRMGVQVAWKTPSWDLITAGGWNDRWDLSVGSMTITKERAQVLDFSTPYYYTPAGLAV
                     RDDSDITSFDQLAGKTIGACSACTYEFYLERKLDIPGYTFDYLVPANIKFVGYDTDST
                     AIQDLVLGRLDAAMSAVPTLQGAIDKGKPIKLLGDPVFYEPLAAAADKSAPLDPTSFI
                     QQVSDIIQQMHDDGTLTHLSMKWYGEDITKPQA"
     gene            complement(1339..2523)
                     /locus_tag="VE646_08710"
     CDS             complement(1339..2523)
                     /locus_tag="VE646_08710"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HZD80107.1"
                     /translation="MRSKRPLIAWLLALVVLAGACTTRAPARTPAPAPTTSDPSPAPS
                     MLKIAFIEDLAPEDAQTRTAPAYQGARLAVDTATLGERLPVPVEIVALDTKGDPSTAA
                     DVVRTIVDDQSFVGAIGAPLLTGQASIGVAMDAAGLPMITLSTLGPDLSRNGWTGWRR
                     AVAGQTREAAVLADHIDGMRRLGAGACLIGDGSPASIGLLHGVARAISTPVALRMRAA
                     IERNGSDRVVAAIAAAGCGVVVWGGFGAEGAVLRQQLVEQGLRSVRFVGGEGLKDPTF
                     LSVVGEAGAGTVVVCPCVDLTTSTRFAAQRFIQDYQSDFGLPPGPYAAEAWDVMRMFL
                     QAIERGAATRSQVAAALSATSSFEGLADTYVFEPSGELVPSSAQVHVFRDEGGRWIPQ
                     TG"
     gene            3029..3112
                     /locus_tag="VE646_08715"
     tRNA            3029..3112
                     /locus_tag="VE646_08715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3063..3065,aa:Leu,seq:caa)
     gene            complement(3139..4293)
                     /locus_tag="VE646_08720"
     CDS             complement(3139..4293)
                     /locus_tag="VE646_08720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZD80108.1"
                     /translation="MSETADVVVVGGGVVGTSAAYHLAAAGAGRVLLLERADGLGMGS
                     TGACAGGFRFQFSSRINIELSLASVPMITRFTEEHGLPLDVWRDGYLFLIRDDETWAG
                     FSAAAELQRSFGIDVRAISAAEADRLVPGISVEGVVGATFGPDDGIADPAGLTNGYAM
                     LARRAGVQIRTGVDVTEILTHAGAVTGLTAAGASIGCPMLVDAAGPWAGLLAATCGVE
                     VPLEPIPRNVVVTGGFPGRPERRTLVIDAKTSFYFHREGDGVLMGMGSPTERPSFDTT
                     PDRRFVEEELFPTAVRVFPPLAEAEVRTMWNGLYEMTPDRHPILGPVEGLEGLYLANG
                     FSGHGFQHAPIVGKLLAEMIVDGAAKSVDVSALSLERFSRGDLVPESHVI"
     gene            complement(4290..5219)
                     /locus_tag="VE646_08725"
     CDS             complement(4290..5219)
                     /locus_tag="VE646_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HZD80109.1"
                     /translation="MRDLPLVDAARLSELLPMTSAIDALEAAFRSGPPSGTPLRSQVE
                     TPAGTLLSMPAAGPQGAGVKLVTVTPSNPARGLPLIHGAYVLFASQTQAPVAVLDGGA
                     LTALRTGAVSGLATRYLAREDADRLVIFGAGVQGRAHLEAMRAVRSIERVTVVSRTDA
                     RAEALADLAEAMGLEAVTGEPDAVGEADLVCTCTTSPAPVFEGTRLREGAHVNAVGAY
                     TPDTRELDEETIRRSRLVVEAREAAMAEAGDLLIPIAEGIVDAAHVVADLSEVVRGAL
                     VRRSPQDVTVFKSVGVAFEDLVVARAAVDRFVS"
     gene            complement(5222..5959)
                     /locus_tag="VE646_08730"
     CDS             complement(5222..5959)
                     /locus_tag="VE646_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD80110.1"
                     /translation="MSDPGSDGAPILELDGVSTHYGLLSILRDIHVEIRPGEIVCLLG
                     GNASGKTTTLKTILGMVTPSQGEVRLAGERISGLPTSEVVRRGITMVPENRRLFTTMT
                     VRENLDMGAYQRTDKKAVARDLERILDTFPRLRERLSQKAGTLSGGEQQMVAMGRALM
                     AQPKVLLMDEPSMGLAPVLVDQVFQIIREINQLGTTIFVVEQNANMALSIAHRGYVLQ
                     TGEVVLSDTARNLLDNPQMREAYLGEL"
     gene            complement(5956..6741)
                     /locus_tag="VE646_08735"
     CDS             complement(5956..6741)
                     /locus_tag="VE646_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003389436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD80111.1"
                     /translation="MTALLEVGGVSKHFGGLYALAGLDLEVQRGEIVSVIGPNGAGKT
                     TFFNTITGLYRPDEGDVRFDGESLVGLTPNQITRLGVARTFQTVHLFANMTVLENVMV
                     GQHARTRSGVLGAVLRLPGTRAEEERVRSRAKEILEFFGSRLQGYRHDQPAWVLSYAN
                     RRRLEIARALATEPQLLLLDEPTAGMNPRETLELTEQIASLRDLGYTIVLIEHDMRVV
                     KGVSDRVIALDYGKKIAEGTYETVANDPQVVEAYLGRKAADGG"
     gene            complement(6738..8513)
                     /locus_tag="VE646_08740"
     CDS             complement(6738..8513)
                     /locus_tag="VE646_08740"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine/isoleucine/valine transporter permease
                     subunit"
                     /protein_id="HZD80112.1"
                     /translation="MTGALLGRAARTGLLAGLVLVYLGLVGMIEKFGARNLVGEVLTL
                     SRLLIVAPPFLGGYLLVRTRVSKGQIDRPMPGPGIAGGAVAGAVAGVVVVLAILVAKG
                     IGIDTIRGVFIEVTQGLISTLTFGRDLGSGSVMLVLVGAAGGAVGSAYRYLPARRARP
                     LAVATTVVLLAAVLQRIVPTVLFQLGLDTAWLYDPFTLGLTYLGAAILFVVSGGAVLL
                     WIDRRSEVRDRMLTWSPQGRRTMVGLWIAVVLFVLAVLPQLLGSVLSQILGSVAIYVL
                     MGLGLNIVVGYAGLLDLGYVAFFAVGAYATALFTGGLLITSVGQAAPAISLHLSFYAA
                     VPLVIAFAAFVGVLIGAPVLRLRGDYLAIVTLGFGEIASRLAASDWLKPVVGGAQGMR
                     RITDASIFGISFRDPQNFAYLAIAFSILAIFVSVRLADSRIGRAWQALREDEQVAEAM
                     GISTTKFKLLAFALGGGIGCLSGALFAVQIGSLNPTSFEILVSITVLAVVILGGMGSI
                     PGVIVGAMVLIGIPGLLSEFEEFKLLIYGAVLVGIMVLRPEGLVPNIRRATELKEEER
                     AQDQWLRDVGSAPATEEGAAAREPA"
     gene            complement(8515..9633)
                     /locus_tag="VE646_08745"
     CDS             complement(8515..9633)
                     /locus_tag="VE646_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZD80113.1"
                     /translation="MAKLTGGPGIATGGAATTQARIARFPIRQSIQWILIMAIALGVG
                     LGAWKTLALPSGQRYSLDAWREFIVLGVAQGAIYALIALGYSLVYGILLMINFAHGEV
                     FMFGAYGSFFVARALDANGILNRHPIPTLVLLLVVAMIFSTLVAILLERIAYRPLRNA
                     PRLVPLITAIGASLFLQNTARGFFGNQTQGYPEFDVFTNKSWTILGVPIGRVQVVVIL
                     TSLVAMIALQFFVARTKTGRSMRAVSEDREIAALMGVNVDRVIMVTFAVGGVLAGIAG
                     VLFAITFSQVNFFMGFVPGIKAFTAAVLGGIGSIGGAALGGFILGLLESVGPPLFLSG
                     AHVPSPFQLKDVIAFTILVLVLIFRPGGILGSGEQEKV"
     gene            complement(9739..11022)
                     /locus_tag="VE646_08750"
     CDS             complement(9739..11022)
                     /locus_tag="VE646_08750"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HZD80114.1"
                     /translation="MVAAACSNSSNNNASGGGGGNAADCNADQFGCVTYKSGEPIKLG
                     TLLAISGDTASLGLDSQHGVALAIDYLDGSFDGTGGQIDGHDITLVNEDDGCSAEGGQ
                     AGATKLAADPQIVGVIGTSCSSAALGVADKITSDKGIVMISPSNTNPNLTAEGTHQPF
                     YLRTAHNDKIQGAIVADFAIQKLGAKTAATIHDESPYADALAAVFRDNFQQQGGTIVP
                     NGDQAVQSTDTDLKPLLTSIGQDKPDVLYFPDFNPVCALISKQSAGVSGLENTALIGS
                     DGCLATDYLKIAGSAADGDYASSPDLSVFANGDFYKNQFIPAYKDQFGGAPTSVFHAH
                     AFDAANILFEAIKKVAVQGSDGSLQIPRTALKDAVFATSGYQGMTGTITCVPLGDCAT
                     DVTIAVFKAPDWPVEGGNPDAKPVFTETKTLQDVS"
     gene            complement(11216..12169)
                     /locus_tag="VE646_08755"
     CDS             complement(11216..12169)
                     /locus_tag="VE646_08755"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZD80115.1"
                     /translation="MVVRGLLSGVEVGLVLALALFAINYSRIELVREVPFGSTHRSNV
                     DRPASERLALETLADRVQILRVSGFVFFGTASGLLERIRKRAEGRPIPFLLVDLRRVT
                     GMDSSAVLSFRKLVQFAEGDRVQLIFSGASQRVLGQLRRGGVAASAGVVSFVPDLDQG
                     LEACEDGLLVSDEWIGRERGGGQSMGELRDRLGAYVERVSLPEGAVLIHQEQPPDDVF
                     VLESGRLKVEMTTPEGERLRLRSMRAGVVVGEVALYTGVPRTADVVAETPSVVLRLSQ
                     ASIDRMEAADPELAVELHRWLATTLAERLRYTLNAFDTLFD"
     gene            complement(12714..13379)
                     /locus_tag="VE646_08760"
     CDS             complement(12714..13379)
                     /locus_tag="VE646_08760"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HZD80116.1"
                     /translation="MSEHPSSEGALAVITAGAVIGAVEVVLAVSFAALVFGGYLVAFL
                     ANGIGLYLLAAALTLAILAWRAGARGVVGSVQDAAAAVLAIVATSTAVGAFGSPDRAF
                     LSVVAATLVVTLLTGLTFFLLGTFRLGNLVRYVPYPVVGGFLAGTGWLLAKGGVGVAG
                     SVVPSVGSLGQMMKPYELIRWVPAVVFGLTLVLVTRLVKRPVILPVEPSASGSRRSRS
                     ACS"
     gene            complement(13471..14241)
                     /gene="trpA"
                     /locus_tag="VE646_08765"
     CDS             complement(13471..14241)
                     /gene="trpA"
                     /locus_tag="VE646_08765"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZD80117.1"
                     /translation="MADLEAALRDRLAGGGRSFVPYVTGGLEGVDADLLRALQEIGAD
                     ALEVGLPFSDPVMDGGVIQEASRRALEAGTRPTDVLTTVGEAGLSIPVALMTYANPIL
                     RHGPDRFLADASAAGVSAMIVPDLPVDEADAWVGLCAAAHVAPVFLAAPGSSSDRLRR
                     IAECSRGFVYCVATYGVTGERERLAGTARELVASLRPLTDRPLLVGVGIGTPAQAAEA
                     CAFADGVIVGSALVRRLVEGDRDGALELARGFRSAIPG"
     gene            complement(14234..15436)
                     /gene="trpB"
                     /locus_tag="VE646_08770"
     CDS             complement(14234..15436)
                     /gene="trpB"
                     /locus_tag="VE646_08770"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015110393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HZD80118.1"
                     /translation="MSGAPVVLPDARGRFGRFGGRYVPEILIPALDELAAAWAELKDD
                     PSFRGPLGALLRDYVGRPSPLTFAARLSDRLGYRVYLKREDLNHTGAHKINNTVGQVL
                     LATRLGKRRIIAETGAGQHGVATATACALFGLPCVVYMGEEDTVRQAPNVSRMQLLGA
                     EVVPVSSGSRTLKDAMNEAMRDWAATVSDTHYVIGSVAGPHPFPALVRDLQRVIGDEA
                     RVQFRQAEGGDPQVVLACVGGGSNAMGIFAPYVGAAGVRLIGVEAGGRGEGPGDHCRS
                     LTLGEPGVLHGSMSYLLQDADGQVVPTHSISAGLDYPGVGPEHAYLKEAGLAEYVSAT
                     DAQALEGFQALAELEGILPALEPSHAVGWLLDRPLPEGSSVLLNLSGRGDKDLDSVRA
                     ALGEVLVG"
     gene            complement(15433..16209)
                     /locus_tag="VE646_08775"
     CDS             complement(15433..16209)
                     /locus_tag="VE646_08775"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HZD80119.1"
                     /translation="MGFLSDLLGDVRRDLDRRPIDVGPLMGRIALLPPARGFARSLRA
                     SEAPAVIAELKRSSPSAGLIGDADPGVQAAGYERGGAAAVSVLTEPRHFGGSLVDLRA
                     VRMATSIPVLRKDFLIHPDQLIQSRAEGADAVLLIAAALERSELVAMLAAAADLGLDV
                     LAEAHTEQDLEAVVTSGAEVVGVNARDLETLDVDLDRAIGLLRQVPEDRIAVLESGIG
                     SPDRVRRAADAGAGAVLVGEALMRSGDPERALRALRGAPA"
     gene            complement(16275..16997)
                     /locus_tag="VE646_08780"
     CDS             complement(16275..16997)
                     /locus_tag="VE646_08780"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HZD80120.1"
                     /translation="MGFEVIPAVDVSDGRLTRMGPAGPEPVDAFHGDPLAAAAAFLEA
                     GAVWIHLVDVDRALTGVPRDLALLRRVAGLGARVQASGGIVSLDDAEGALEAGAARVV
                     IGSGALSDLHGLERLLVAVGPEAVVGIEIRDGSIRPRGGEAVRLPLPATLERLARLPV
                     RRLLCTAVARVGELTGPDLSGLRLVAEETGRPVIAAGGVRGPEDLRAVADAGLEGAVI
                     GRALYEGLDLRRAREAVFTGGG"
     gene            17226..17585
                     /locus_tag="VE646_08785"
                     /pseudo
     CDS             17226..17585
                     /locus_tag="VE646_08785"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            17758..18123
                     /locus_tag="VE646_08790"
     CDS             17758..18123
                     /locus_tag="VE646_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80121.1"
                     /translation="MLERAPSPERGRRLTVSQILARLRRGGRRRNLKLRGEEISAALR
                     SPQLEAPPVLTGAYAARVHALVAVLQEMAAQIESLEQELRRSFRSHPDAEIYLSLPGL
                     GDVPGPSASSAMIRPATRS"
     gene            complement(18452..19228)
                     /locus_tag="VE646_08795"
     CDS             complement(18452..19228)
                     /locus_tag="VE646_08795"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZD80122.1"
                     /translation="MNELRPATPAVSEPSTLVTQDGVVLRARRWEPPRSLGTSVILAH
                     GLSSSKDDPRLVAVAKALAADGYDVLSYDARGHQDSGGLCTLGDRERLDVGAAVAWAR
                     ERSDHVVPVGASMGAIAALRYGVSDPLLDGLVSVSCPAVWRLPRNARALAATGMTRTR
                     AGRWFARHQMHVRLAREWLDPEPPGVLAVRLVVPLAVVHGGRDRFVAPREAVRLASAA
                     PRARLILVPEMGHAFDPSAVEAIRTAVRWVVSESTRPATA"
     gene            complement(19225..19875)
                     /locus_tag="VE646_08800"
     CDS             complement(19225..19875)
                     /locus_tag="VE646_08800"
                     /inference="COORDINATES: protein motif:HMM:NF041646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HZD80123.1"
                     /translation="MQGSFQIPRLSALARHAIPHVVEGTLMPLAIFYLALWLVGVWGA
                     LIGALAWSYAALAVRLILGKRVPGLLLLGTAAITVRTLISFLTGSVFVYFLQPTLGTV
                     AIGLAFLLSVPVGRPLAERLAGDFVPLPHALASHPVARKVFLRITLLWAFVLLSNAAV
                     TLWFLLTEPVGVFVMAKTASTAVFTVGAVALSSFWFRGTLRRHGLLAPKRPVGSRA"
     gene            19975..21642
                     /locus_tag="VE646_08805"
     CDS             19975..21642
                     /locus_tag="VE646_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HZD80124.1"
                     /translation="MQTSGRLGPADLRAAFVTYREILHAHRQVINRLNVFPVPDGDTG
                     TNLSLTVDAVVAELESDAGMEATARAIGHGAVMGARGISGVIAAQLFRSAVDHLAVAP
                     EVDGASLAAALDAAARAAYGAVSRPVEGTILTVAREAAAAAGSAAGEGDLLTVIQAAR
                     RAAWEAVERTPDLLPVLRDAGVVDGGGTGFALFFDALLHVVAGTPLPDPSDRAADPVL
                     TAWSRWSSAPDGAGDGAVSAHPSDARPPGGDHGDRRFEVALLLEAPDEAVPAFRDVWN
                     RLGGAVVVVGGGGRWSAHVHTDHPGSAVDAAKSFGRPSDVRVTDLALQIVEERGDLAA
                     PQERTRCGAVAVGAGDGIHRLFRSLGARVVTGIGSPNPSVRELVEAVESVASDEVVVL
                     PNDDDVAPAAEQVARLSGKRVVVVPTRGIAHGLAALRAFDPDAPAEENAEAMSEAARS
                     VSFGRLVRAVRDAGSEVGDIHEGDWLGITAEGIRVVEPEVRQAGRALLERLLSDRSEI
                     VTIIEGEGATEEVTRDLTDWLQAIRPEIGVQVLHGGQPIHAYLFGLE"
     gene            complement(21673..23088)
                     /locus_tag="VE646_08810"
     CDS             complement(21673..23088)
                     /locus_tag="VE646_08810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZD80125.1"
                     /translation="MSGGVPRAAVRSWWMEEALAHDPGEPCPPLSGDTSADVVVVGGG
                     YTGMWAAEFLKQVDPGIDVVLLEQDICGGGPSGRNGGFVNGLWDDLGLLIERYGPEGG
                     MAVARAAGQEIDGIGAWCQEHRVDAWYAKDGDLGISASPAQDAVLDSVIREAAETGIG
                     ELYQRLSADEARAHCASPLLRGAVWIPDAAKVQPARLARGLRRVLLDRGVRIHEGTPV
                     RRFHAGPRIVAETPGGTVTAGHAVLAVNAWGMHWRAFRRAVMVRGTFIVITEPAPERL
                     AELGWTGGEGLYDFRTALHYLRTTPDGRIAFGGAGMQVASRRRIGPRFLYEERSIRRL
                     VEDLHRWFPPFRTVPLEAAWGGPIDVSGRHVPFLGTLPDGRSHFALGFTGNGVGPSRL
                     AGRILASIAAERPDALASLPIVDDRPKAFPPDPVFVPGERLVTAAVLRKDDLEDDGRR
                     PGPITRALAHLPRRLGYNLGP"
     gene            complement(23085..24518)
                     /locus_tag="VE646_08815"
     CDS             complement(23085..24518)
                     /locus_tag="VE646_08815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZD80126.1"
                     /translation="MNSTRTDAPPRADRSWWLQEALAQPAFAGEPCPPLAEDLAADVV
                     IVGGGYTGLWTAYFLKERDPGIEVVVLEQDICGGGPSGRNGGFVNGWWTSIGEMARRF
                     GDADAMELCLAASRSVRGVGDFLRRHDIDAWWSPNGELAVASSPFHEGAWTAMLEEAR
                     HHGVEDEFEELTPSEVTDICISPMFGGGVLSKEFSTVQPARLARGLRRVVLERGVRIF
                     EQTPVRGFGAGRPVVAETPGGTVRAGEGVLAVNAWGIHWKRLHPYVGVRGSYMVLTAP
                     APARLEEINWTEGQGIRDFRPSLHYLRTTPDGRIAFGCAGMQPGLSRRIGPRFAYDVG
                     FLRRAVGDLHRMFPSFRDVPIEAGWGGPIDVAGLYLPFFGSFPSGTVHYGLGYTGNGV
                     APAHLGGQILASLALHAEDGFSRLALVTNEPLRLPPEPLLSPGMLMVNEAIRRKDDAE
                     AEGRRAGPLTTFVARIPRRLGYNLGPR"
     gene            24575..26251
                     /gene="ilvD"
                     /locus_tag="VE646_08820"
     CDS             24575..26251
                     /gene="ilvD"
                     /locus_tag="VE646_08820"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZD80127.1"
                     /translation="MGADNRPRSRQVTEGPERAPARAMLRAVGFSEEDFGKPQIGVAS
                     SWNEVTPCNYHLDKLAALAKEGVRQGDAVPIEFTTIAVSDGIAMGHEGMKASLVSREV
                     IADSVELVMHAERMDGLVGIAGCDKSEPGMLMAMARLNLPAVYLYGGTILPGTYRGRD
                     ITIQDVFEAVGANARGGMSDEELLAVERAACPTTGSCAGMYTANTMAAAAEALGMSLP
                     GAASPPAVDYRREVFARGSGVAAAKLLELGIRPRDIMTKPALENAIAVVMALAGSTNA
                     VLHLIAIGHEAGVDLRLDDFDRISRQVPHLVDVRPAGRFVMSDLDRAGGVPVVMKELL
                     EAGMLHGDCLTVTGETVAENLAAMEIPEPDGDVVRPVSEPIHAEGGTAILHGSLAPEG
                     SVMKIAGAEGLDFRGTARPFDSEQAAFDALTSGRLKAGDVIVIRYEGPRGSPGMPEML
                     AVTAAVAGAGLGADLALITDGRFSGATKGYSVGHVAPEAFVGGPIALVRDGDQIVIDA
                     ANRRLDLLVEEAELDRRRAAWSAPEPRYATGALAKYARLVGSASRGAVTS"
     gene            26248..27483
                     /gene="thrC"
                     /locus_tag="VE646_08825"
     CDS             26248..27483
                     /gene="thrC"
                     /locus_tag="VE646_08825"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZD80128.1"
                     /translation="MTRLTEPPVLECRECGARYPVEPLTICEECFGPLEPWYDLSAID
                     GTDFRNHAEAGPPTLWRYEMLLPGGPGVRRVDLGAGFTPMRRAERLGERLGIEKLWIK
                     DDSGNPSNSFKDRVVSVAITVARAFGFEAISCASTGNLANATAAHAAKAGMPAYVFVP
                     DDLEVAKVLATEAYGAKVVAVDGSYDDVNRLCSEVAEALPWAFVNVNMRPFYTQGSKT
                     LGFETAEQLGWRLPDHVVVPIASGALLTKVHTAFQELIAIGAVEETPYRISGAQPEGC
                     SPVAQAFAAGTEEVKPVKPNTIARSLGIGNPADGYYAIRIANHTGGAIDACAEDEVIE
                     GIRLLAETEGVFTETAGGVTIAVLKRLREAGVIEADHETVALITGNGYKTIEALQGTL
                     EPTYHVEPDLDAFLAALGP"
     gene            27584..28387
                     /locus_tag="VE646_08830"
     CDS             27584..28387
                     /locus_tag="VE646_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZD80129.1"
                     /translation="MADVLRVQGAEVRIGGRRILGPLSLCIDPGERWVVLGSNGSGKT
                     TLLRLAGALRQPSAGTVEVLGERLGATDVRALRARIGFVGHAVADELPPGLSVVDAVL
                     TGKRATLVTWMQQLGHADRRRAAVLLEHVGCAQLEGQPFRQCSQGERQRVLLARALFG
                     NPELLLLDEPAAGLDLPGRESLIRALEAADDLGSTPSVLVTHHLEEIPPTTTHAALLR
                     AGEVVVQGPVDEVLLDEPVSACFDLPVRVGRKGSRWSAVSLERPTGHLA"
     gene            28623..28946
                     /locus_tag="VE646_08835"
     CDS             28623..28946
                     /locus_tag="VE646_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80130.1"
                     /translation="MRYEFERDGRTVASVLWEGPAQVTVDVADPADRPQFDRFFGAEV
                     VYMDAGFDFGGGMSESGLTSRRRDWTPWEFERACRNLAHKLGCTVHRVETGPVEARPE
                     EVASR"
     gene            28943..30688
                     /locus_tag="VE646_08840"
     CDS             28943..30688
                     /locus_tag="VE646_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019664313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HZD80131.1"
                     /translation="MRVTGAQALFKALEGEGVDLVFGIPGGAILPAYDPLIDSSIRHV
                     LTRHEQGAGHAAEGYAWATGKVGVCIATSGPGGCNLVTPLADAKMDSVPLVAITGQVN
                     SAAVGNDAFQEADITGITMPVTKHNFLVKDPGDVAETIREAFHIASTGRPGPVLVDLP
                     KDILIEETEWTWPERVELPGYKPTTKGNLKQVREAVRSIMAAERPVLYVGGGVIKANA
                     TKELFELATEGNLPVVTTLMARGAFPDDHELAFGMPGMHGGYTAVTALQKTDLLVALA
                     SRFDDRVTGQLSTFAPNAKVIHLDIDPAEIGKNRRADVPIVGDARDVISKLVAELRRR
                     QEEAGGPPDRSGWLGVLRSWMERYPYTYEQPDDGPLKPQFAIQRLYEHARDAIVVAGV
                     GQHQMWASQFWRFNEPRTWINSGGLGTMGFAVPAALGAAAGRPDRRVIAIDGDGCFQM
                     TAQELATSSTEGIPFVTAIMNNGYLGMVRQWQELFYRERYSQVELTQTTPDYVRLAEA
                     YGAVGLRAEHPDEVDPVIEKALSVEDRSVVIDFRCDPGEMVFPMVPAGASNDDIIVGP
                     GGLRPVDDPAEAPIA"
     gene            30685..>30882
                     /gene="ilvN"
                     /locus_tag="VE646_08845"
     CDS             30685..>30882
                     /gene="ilvN"
                     /locus_tag="VE646_08845"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HZD80132.1"
                     /translation="MNGNGSLHTISVLVEDRPGVLTRVAGLFAARGFNIDSLAVGPTE
                     ERGLSRMTLVVNVESKPLEQVG"
BASE COUNT         4803 a        11169 c        10646 g         4264 t
ORIGIN      
        1 cgaagaagat cgcgcagacc gcgatgaaga ccgtgaccca cagcccacgg gtgatgaacc
       61 agaagttgtg gcgcatccag gcgacgttca gttcgaacgc gaccaagacc ccgcccatca
      121 cggcgaagat ggcgatccaa accgcgatca gcttggcccg gaaggggatc cgctcgctca
      181 ggagctcgta gcgctcgagc aggcccgtcg ccctggcccg agcaccgggg ggaggggtgg
      241 tcccggcccc cgtctgctcg gtcttcatga tctctcagag ccttcctacg cctgaggctt
      301 cgtgatgtcc tccccgtacc acttcatcga gagatgggtc agcgtcccgt catcgtgcat
      361 ctgctggatg atgtcgctga cctgctggat gaagctcgtc ggatcaagcg gcgcgctctt
      421 gtcggccgcc gcggcgaggg gctcgtagaa gaccgggtca cccagcagtt tgatgggctt
      481 gcccttgtcg atcgcgccct gcagggttgg caccgctgac atcgcggcat cgagccgacc
      541 cagcaccagg tcctggatcg ccgtcgaatc ggtgtcgtag cccacgaact tgatgttcgc
      601 cggcaccagg tagtcaaagg tgtagccggg gatgtccagc ttgcgctcga ggtagaactc
      661 gtacgtgcag gcgctgcacg ctccgatcgt cttacccgcg agctgatcga acgacgtgat
      721 gtcgctatcg tctcgcaccg ccagcccggc tggcgtgtag tagtagggcg tggagaagtc
      781 cagaacctgt gcccgctcct tcgtgatggt catcgagccg accgacaggt cccagcggtc
      841 gttccacccc ccggccgtga tcagatccca cgacggcgtc ttccaggcca cctggacgcc
      901 catccgcttc gcgatctcct cggccgcgtc gatgtcgaag ccctcccatt ggccggtggc
      961 ctcgtcgtag gaggactgcg gcgggtacgc cgggtcggtg gacacgcgca cttcgccgtt
     1021 ctggcagatc cgcgccaaga ggtcgccgct gctagtgtcg accgacgcgc acgtgccctg
     1081 agcggccggg cttgcggtgt cgcctccgcc gccggcagcg ttgttgtcgt tcgaagaccc
     1141 gctggagcac gcggccgcga ccagcgtgag cgaggccgtg agcaccacgg ggatcaggaa
     1201 cctggatctg cgcatctccc ccctccttgc cccgtgggga gctgcgccgt tggacgggcc
     1261 ccccgcgggc ggttctccga cgtccgcatg tctccgcgga cggccccgag catagtcccc
     1321 gggtggaagg gacgaaagtc aacccgtctg tgggatccag cggccgccct cgtcgcggaa
     1381 gacgtggacc tgcgctgagc tcggcaccag ctcccccgag ggctcgaaga cgtaggtgtc
     1441 ggccaagcct tcgaaggagg acgtggcgga gagcgccgcc gcgacctggc tccgggtcgc
     1501 tgccccccgc tcgatcgcct gcaggaacat ccgcatcacg tcccacgcct cggcggcgta
     1561 ggggccgggg ggcagcccga agtccgactg atagtcctgg atgaaccgtt gggccgcgaa
     1621 ccgggtcgag gtggtgaggt ccacgcaggg gcacaccacg accgtgcccg cccctgcctc
     1681 tcccaccacc gagaggaagg tcgggtcctt cagaccctcg ccgcccacga aacggaccga
     1741 gcgcagcccc tgctccacca gctgctggcg gagcaccgcc ccctcggcac cgaacccgcc
     1801 ccacacgacc acgccgcagc ctgcggcggc gatcgcggcc accacccggt ccgagccgtt
     1861 ccgctcgatt gcggctcgca tccggagcgc caccggtgtg gagatggccc tggccacccc
     1921 gtggaggagg ccgatcgagg cggggctacc gtccccgatc aggcacgctc ccgctccgag
     1981 gcgccgcatc ccgtcgatgt ggtccgccag caccgccgcc tcccgcgtct ggccggccac
     2041 ggcccgtcgc catccggtcc agccgttccg cgacagatcc gggccgagcg tggacagggt
     2101 gatcatgggc agtcctgccg catccatggc gacgccgatg ctcgcctgtc cggtcagcag
     2161 gggggcgccg atcgcaccga cgaagctctg gtcgtcgacg atcgtccgga cgacatcggc
     2221 agcggtcgat ggatcgccct tcgtgtcgag ggcgacgatc tccaccggca ccgggagtcg
     2281 ctcccccaag gtggccgtgt cgacggccag tcgggcgccc tggtacgcgg gcgcggtcct
     2341 ggtctgagcg tcctcggggg cgagatcctc gatgaaggcg atcttcagca tcgacggggc
     2401 cgggctggga tccgaggtcg tcggcgcggg agccggggtc cgtgcgggcg cgcgtgtggt
     2461 gcacgcgccg gcaaggacga cgagcgcgag cagccaagcg atgagcgggc gcttgctgcg
     2521 catcgggcga ggaaagcctc ctggctcgct gggccgacgg gtccggcagt ctagatctca
     2581 gagcctcgta ctaccctgct cccacgactg ggaggttcca tcttgtatcc gcgtgcaacc
     2641 tacgaccatg tcctgcgact cgttcacgcg ggctcaacga cagccaggtc tcgcgccaga
     2701 ccggtatctc gagatggacg gtggggaggt cggcctcgtt cccgagccgg gatgcgtcgc
     2761 cgtatccaca tactggcagc actggccatg cgtgttccct caacacggcc ccggcatgaa
     2821 acacaccagg acgatcgcct tggcggcttg gcagcaggac gtggtaaccc gcgagcctga
     2881 ggggttgctg cgtggcctga tccactctga tgggtagcgc tggaacggcg tgaccatctc
     2941 gatcgcgcgg cgcgccgacg tggccacgtt ggataccttc gctggtccca aggcctgatg
     3001 catctcgcat gagtacactc cgagggacgc cgggatggcg aaacgggaaa acgcgcggga
     3061 cccaaactcc cgtgggcttc ggcccttgag ggttcgactc cctctcccgg caccgggttc
     3121 gagtcccgcc cccggcactc agatgacgtg ggattcgggg accagatccc cgcggctgaa
     3181 ccgctccagc gacagcgccg acacgtccac gctcttcgcc gcgccgtcca cgatcatctc
     3241 ggccaacagc ttccccacga tcggcgcgtg ctggaacccg tggccgctga acccgttcgc
     3301 caggtacagg ccctcgagcc cctccaccgg acccaggatc gggtggcggt cgggggtcat
     3361 ctcgtacagg ccgttccaca tcgtgcggac ctcggcctcg gccagcggcg ggaacacccg
     3421 aaccgcggtc ggaaacaact cctcctccac gaaccgtcgg tcgggtgtcg tatcgaacga
     3481 cggccgctcg gtcgggctcc ccatgcccat cagcacgccg tcgccctcac ggtggaagta
     3541 gaagctggtc ttcgcgtcga tcaccagcgt ccgtcgctcg ggcctccccg ggaatccccc
     3601 cgtcaccacc acgttgcggg ggatcggctc cagcggaacc tccacgccgc aagtcgccgc
     3661 cagcagcccg gcccaggggc cggccgcgtc caccagcatc ggacacccga tcgatgcacc
     3721 cgccgccgtt agaccggtca cagcgcccgc gtgggtcagg atctcggtga cgtccacacc
     3781 ggtccggatc tgcacccccg cccgcctcgc cagcatcgcg tagccgttcg tcagcccagc
     3841 cggatcggcg atcccgtcat ccgggccgaa cgtcgccccg accacgcctt cgaccgagat
     3901 gcccgggacg agccggtcgg cctcggccgc tgatatggcc cggacgtcga tcccgaacga
     3961 gcgctgcaac tcggcggcgg ccgagaaccc ggcccaggtc tcgtcgtcgc ggatcaggaa
     4021 caggtatccg tcccgccaca cgtccagcgg cagcccgtgc tcctcggtga accgggtgat
     4081 catgggcacg ctagccagcg agagctcgat gttgatccgg ctggagaact ggaaccggaa
     4141 cccgccggcg cacgcccccg tcgatcccat accgagcccg tcggcccgct ccagcaggag
     4201 gacccgcccc gctcccgccg cggccaggtg gtacgcggca ctggtaccca cgaccccgcc
     4261 gccaaccacc accacgtccg cggtctcgct cacgacacga accggtccac ggcggctcga
     4321 gctacgacca ggtcctcgaa ggccaccccc accgacttga acaccgtcac gtcctggggt
     4381 gaccgacgca cgagggctcc gcgcacgacc tcgctcaggt ccgccaccac gtgggcggca
     4441 tccacgatcc cctccgcgat cgggatcagg aggtcaccgg cctcggccat ggccgcctcc
     4501 cgagcctcca ccaccagacg ggatcgccgg atggtctcct cgtccaactc ccgggtatcg
     4561 ggggtgtagg cgccgaccgc gttcacgtgc gctccctctc tcaaccgtgt cccctcgaag
     4621 acgggggccg ggctcgtggt gcaggtgcag accaggtcgg cctccccgac cgcgtcgggt
     4681 tcccccgtca ccgcctccag ccccatcgct tcggccagat cggccagcgc ctccgccctc
     4741 gcgtccgtcc gcgagaccac cgtgacgcgc tcgatcgatc ggaccgcccg catggcctcc
     4801 agatgcgccc gaccctgcac ccccgccccg aagatcacca gccggtcggc gtcctcgcgc
     4861 gccaggtagc gcgtggccag ccccgagacg gcgccggtcc gcagggcggt gagcgcacca
     4921 ccgtccagga cggccaccgg cgcttgcgtc tgggatgcga acaggacata cgctccgtgg
     4981 atcaggggga ggcctcgggc cgggttcgac ggcgtcactg tcaccagctt cacccccgcc
     5041 ccctgcggtc cggccgcggg catcgacagc agcgtccccg ccggggtctc cacctgcgat
     5101 cgcaggggcg ttccggacgg gggccccgaa cggaacgccg cctccagcgc gtcgatcgcc
     5161 gaggtcatcg gcaggagttc ggacagccgt gcggcgtcca cgagcggcag atcgcgcacg
     5221 ctcacagctc tcccaagtac gcctcccgca tctgggggtt gtccagcagg ttccgggccg
     5281 tgtccgagag caccacctcg ccggtctgga gcacgtagcc gcgatgggcg atcgacaggg
     5341 ccatgttggc gttctgctcc acgacgaaga tcgtcgtccc cagctggttg atctccctga
     5401 tgatctggaa gacttgatcc acgagcaccg gcgccaggcc catggacggc tcgtccatca
     5461 gcaggacctt gggctgagcc atgagcgcgc ggcccatcgc caccatctgc tgctcgcccc
     5521 ccgagagcgt ccccgccttc tgcgacaggc gctcccgcag gcgcgggaac gtgtcgagga
     5581 tccgctcgag gtcccgcgcg accgccttct tgtcggtccg ctggtacgca cccatgtcga
     5641 ggttctcgcg gacggtcatg gtggtgaaca gacgccggtt ctcagggacc atcgtgatcc
     5701 cgcgccgcac cacctccgag gtcggcaacc cgctgatccg ctcccccgcc agacgcacct
     5761 cgccctggct gggggtcacc atcccgagga tcgtcttcag cgtggtggtc ttcccgctgg
     5821 cgttgccccc gagcaggcag acgatctcgc cggggcggat ctccacgtgg atgtctcgca
     5881 ggatgctgag tagcccgtag tgcgtgctca cgccgtcgag ctcgaggatg ggagcgccgt
     5941 cggacccggg atcgctcatc cgccgtccgc cgccttgcgc cccaggtacg cctccacgac
     6001 ctgcgggtcg ttcgcgacgg tctcgtacgt accctccgcg atcttcttgc cgtagtccag
     6061 ggcgatcact cggtccgaga cgcccttcac cacacgcatg tcgtgctcga tcaacacgat
     6121 cgtgtacccc aggtcgcgca ggctcgcgat ctgctccgtg agctcgaggg tctcgcgcgg
     6181 gttcatgccg gccgtgggct cgtccagcag cagcagctgg ggctcagtcg cgagggcccg
     6241 ggcgatctcg agccgccgtc ggttcgcgta cgaaagcacc cacgccggct gatcgtgccg
     6301 gtagccctgc agacgcgagc cgaagaactc caggatctcc ttggcccgcg accgcacccg
     6361 ctcttcctcg gcccgggtgc ccggcagccg caggaccgcc cccagcacgc cggaccgagt
     6421 cctggcgtgc tgtccgacca tgacgttctc cagcacggtc atgttggcga acaggtggac
     6481 ggtctggaag gtgcgggcca cgcccaaccg agtgatctgg ttcggggtca ggccgaccag
     6541 gctctcgccg tcgaaccgca cgtcgccctc gtcggggcga tagaggccgg tgatcgtgtt
     6601 gaagaaggtg gtcttgcccg ccccgttcgg gccgatcacg ctcacgatct ctccccgctg
     6661 cacctcgagg tcgagcccgg cgagcgcgta caggcccccg aagtgcttgg agacccctcc
     6721 cacctccagc agggccgtca tgcaggctcc cgggccgccg cgccctcctc tgtggccggt
     6781 gccgagccca cgtcccgcag ccactgatcc tgggcgcgct cctcctcctt cagctcggtg
     6841 gctcggcgga tgttcggcac gaggccctcg ggtcgcagga ccatgatccc gaccaggacg
     6901 gcaccgtaga tcagcagctt gaactcctcg aactcggaga ggagcccggg gatcccgatc
     6961 aggaccatgg cccccacgat caccccgggg atgctgccca tgccgcccag gatcaccacc
     7021 gccaggaccg tgatggagac caggatctcg aagctggtgg gattaaggga gccgatctgg
     7081 accgcgaaca gcgcccccga caggcatccg atgcccccgc ccagcgcgaa cgccaggagc
     7141 ttgaacttcg tggtgctgat ccccatggcc tcggccacct gctcgtcctc gcgcagggcc
     7201 tgccaggccc ggccgatcct cgagtcggcc agccgcaccg agacgaagat ggccaggatg
     7261 gagaaggcga tcgccaggta ggcgaagttc tggggatcgc ggaacgagat gccgaagatc
     7321 gatgcatccg tgatccgtcg catgccctgg gctccgccaa ccacgggctt cagccagtcg
     7381 gacgcggcca accggctggc gatctcgccg aagccgagcg tcacgatggc caggtaatcg
     7441 ccgcgcagcc tcagcaccgg cgcgccgatc agcaccccca cgaacgccgc gaaggcgatc
     7501 accagcggca cggcagcgta gaaggagagg tgcaggctga tcgccggggc cgcctgaccc
     7561 accgaggtga tcagcaggcc tccggtgaac aaagcggtcg cgtaggcgcc gacggcgaag
     7621 aacgccacgt accccaggtc cagcagtccc gcgtagccca cgacgatgtt cagtcccagg
     7681 cccatgagca cgtagatcgc cacgctcccc aggatctggc tgaggacgga gcccagcagc
     7741 tgcggcagca ccgcgagcac gaacaggacc acggcgatcc atagcccaac catggttcgg
     7801 cggccctggg gcgaccaggt cagcatgcga tcgcggacct cactccggcg atcgatccac
     7861 aggagtaccg cgcccccgct caccacgaac aggatggccg cccccaggta ggtcaggccg
     7921 agcgtgaacg ggtcgtacaa ccacgcggtg tccagcccca gctggaagag gacggtgggg
     7981 acgatccgct gcaggaccgc ggccaggagc acgacggtgg tggccacggc gagcggtcgg
     8041 gcccgacggg ccgggaggta ccggtaggcg gagcccaccg cccctccggc tgcacccacg
     8101 agcaccagca tcacggatcc cgagccgagg tcccgtccga acgtcaaggt ggagatgagc
     8161 ccttgggtga cctcgatgaa gactccccgg atcgtgtcga tcccgatgcc cttggccacg
     8221 aggatcgcca ggacgacgac cacgccggcc acggcccccg ccacggcgcc tcccgcgatc
     8281 cccggccccg gcatgggacg gtcgatctgg cccttgctga ccctggtgcg gaccaggaga
     8341 tagccgccca ggaacggcgg ggccacgatc aggagcctgc tgagggtgag cacctcgccg
     8401 accaggttcc gggcgccgaa cttctcgatc atccccacca gcccgaggta gaccaggacc
     8461 agccccgcca gcaggccggt ccgggccgcc cgccccagca gcgctccggt cacctcagac
     8521 cttctcctgc tcgcccgagc ccaggatgcc gcccggccgg aagatcagca ccagcaccag
     8581 gatcgtgaag gcgatcacgt ccttcagctg gaacggcgac ggcacgtggg cgccggacag
     8641 gaacaacggt gggccgacgg actccagcag ccccaggatg aagccaccca gcgccgcgcc
     8701 cccgatgctt ccgatccccc ccaggacggc cgcggtgaag gccttgatcc cgggcacgaa
     8761 gcccatgaag aagttcacct gggagaaggt gatggcgaag agcaccccgg cgatccctgc
     8821 gaggacgccg cccacggcga aggtcaccat gatcacccgg tccacgttca cgcccatcag
     8881 cgccgcgatc tcccggtcct ccgagacggc ccgcatcgat ctgccggtct tggtccgggc
     8941 cacgaagaac tgcagggcga tcatggccac gagggaggtg aggatcacca ccacctgcac
     9001 ccgcccgatg gggacaccga ggatcgtcca gctcttgttc gtgaacacat cgaactccgg
     9061 gtagccctgc gtctggttgc cgaagaaccc ccgggccgtg ttctgcagga agagggaggc
     9121 ccctatggcc gtgatgagcg ggaccagccg aggcgcgttg cggagcggcc ggtaggcgat
     9181 ccgttcgaga aggatcgcca ccaaggtgga gaagatcatg gccaccacca gcagcaggac
     9241 cagggtcggg atgggatggc ggttcaggat gccgttggcg tccagggccc gggccacgaa
     9301 gaacgagccg taggccccga acatgaacac ctcgccgtgg gcgaaattga tcatcagcag
     9361 gatgccgtac acgaggctgt accccagggc gatcagggcg tagatcgccc cctgggccac
     9421 gcccagcacg atgaactccc gccaagcgtc caggctgtag cgttggccgc tcggcagggc
     9481 aagcgtcttc caggcaccca gccccacacc gagcgcgatc gccatgatca gtatccattg
     9541 gatcgactgc cggatgggga accgggcgat cctggcctgc gtcgttgctg cgccccccgt
     9601 ggcgatgccc ggaccgccgg tcagcttcgc cattgatccc ccgtcatgcg gtcgcggttc
     9661 atccaacctc ccgaccggcc ggcgggtggg ctcagggccc acccgcggcc gatcgcgggc
     9721 gcttcgggaa cgtcgatctc agctgacgtc ctggagcgtc ttcgtctcgg tgaagaccgg
     9781 cttggcgtcg gggttcccgc cttccaccgg ccagtcgggc gccttgaaca cggcgatcgt
     9841 cacgtcggta gcgcagtctc cgagcggcac gcaggtgatc gtgccggtca tgccctgata
     9901 gccactggtg gcgaacaccg cgtccttcag cgcggtcctg gggatctgga gcgatccgtc
     9961 cgagccctgc acggcgacct tcttgatggc ctcgaacagg atgttcgcgg catcgaaggc
    10021 gtgcgcgtgg aacacgctgg tgggcgcgcc gccgaactgg tccttgtacg cggggatgaa
    10081 ctggttcttg tagaagtccc cgttcgcgaa cacggacagg tccggcgagg acgcgtagtc
    10141 accgtcggcc gcggagcccg cgatcttcag gtaatcggtg gccaagcagc cgtccgaccc
    10201 gatcaacgcc gtgttctcca gccccgagac cccggccgac tgcttgctga tcagcgcgca
    10261 gaccgggttg aagtcgggga agtagagcac gtccggcttg tcctgcccga tgctcgtgag
    10321 cagcggcttc aggtcggtat cggtgctctg gactgcctgg tccccgttcg ggacgatcgt
    10381 gccgccctgc tgctggaagt tgtcccggaa cacggccgcc agggcgtccg cgtacgggct
    10441 ctcgtcgtgg atcgtggcgg cggtcttcgc tcccagcttc tggatcgcaa agtcggccac
    10501 gatcgcgccc tggatcttgt cgttgtgcgc ggtgcgcagg tagaagggct ggtgcgtgcc
    10561 ctcggccgtc aggttcgggt tcgtgttcga cggcgagatc atcacgatgc ccttgtcgga
    10621 cgtgatcttg tcggccacgc ccagcgcggc gctggagcag ctggtgccga tgaccccgac
    10681 gatctgcggg tcggcagcga gcttcgtcgc cccggcctgg ccgccttcgg ccgagcagcc
    10741 atcgtcctcg ttcaccagcg tgatgtcgtg cccgtcgatc tggccgcccg tgccgtcgaa
    10801 ggagccgtcc aggtagtcga tggccagcgc caccccgtgc tggctgtcca ggcccagcga
    10861 ggccgtatca ccggagatcg ccaggagcgt cccgagcttg atcggctccc ccgacttgta
    10921 ggtcacgcag ccgaactgat cggcgttgca gtcggcggcg ttcccaccac cccccccgct
    10981 cgcgttgttg ttgctgctgt tgctgcacgc agcggccacc atggtcagca ccgccgatgc
    11041 tgcgaggagc ctccaccccc acttcctcaa gacacacgcc ccctctcgtc gcgtaccgat
    11101 cccccaccgg gacggctgta gggcgtggtc gtatcacaga tcgacggtcc gagggaagga
    11161 cccagcggtc tgctcaccgg ccccggtact ccgtcgagct ctcttcggat cgccgctaat
    11221 cgaagagcgt gtcgaacgcg ttcagcgtgt atcgcaagcg ttccgccagc gtcgtggcga
    11281 gccaacggtg cagctccaca gcgagttccg ggtcggcagc ctccatgcga tcgatcgacg
    11341 cctggctgag ccggagcacc acgctcggcg tctcggcgac cacgtccgcg gtgcgcggaa
    11401 cacccgtgta cagggcgacc tctccgacca cgactcctgc acgcatcgaa cgcagccgga
    11461 ggcgctcgcc ttcgggtgtg gtcatctcga ccttcagccg accggactcg aggacgaata
    11521 cgtcgtcggg gggctgttcc tggtgaatca ggacggctcc ttctggcagc gagacccgtt
    11581 ccacgtatgc gccgaggcgg tcgcgcagct cccccatcga ctgcccacct ccccgctcgc
    11641 gcccgatcca ctcatcgctc acgagcaggc cgtcctcaca cgcctccaac ccctgatcca
    11701 ggtccggtac gaaactgacc accccggcgg acgcggcaac gccgcctcgc cggagctgtc
    11761 cgaggacccg ctggctcgct ccagagaaga tgagctgcac acgatccccc tccgcgaact
    11821 ggacgagctt acggaaggac aacacggccg aggagtccat gcccgtcacc cgacgaagat
    11881 cgaccagcag gaacggtatc ggtcgacctt cggctcgctt gcggatccgc tcgaggagac
    11941 cgctggcggt accgaagaac acgaatccgc tgacccgcag gatctggacg cggtccgcga
    12001 gcgtctcgag ggccagacgc tcgctggccg gccggtcgac gttgctacgg tgcgtggatc
    12061 cgaacggaac ctcccgtacc aactcgatgc ggctgtagtt gatggcgaat agggcgagcg
    12121 cgagcaccag acccacctcc acgccgctca acagaccccg aacaaccacc gtgacgagga
    12181 tcaggaggac cgatcgcgta ctcgagcccg gggagtgaac gacgaacgtc gatcacccac
    12241 tccgtgatga aggagacgcc gacgaagacg aggacaccgc ccaccaccat ccgcgggatc
    12301 agctcgacga ggccggggcc gaaagcgagt gccgtcagtg gcaccagcgc ggctaccaac
    12361 cctgctcgtc tggcaccaac gtccagccgg tggggcgagc gacgtgaggc tgagggcatg
    12421 gtagccgggg atcccgccga aggctccgga caccaggttc accagtccca catcacgtac
    12481 ttcgcggttc gtatccagat ccgtgtggag catcagctcg gtcccactga cgttgaacag
    12541 ggccgcgatc acggccacga agacggccgt cgcgatcccc gccgcctgtt cgaggaccgc
    12601 tgaccaatcg gcaccggtaa gggctcgtag cgtccatggc tgcaagagcg aggccgatgg
    12661 gaacggcccc aacatccacc ccccttgcag cgcttcgttc ctcgtcgatc cggtcacgag
    12721 catgccgacc gcgaacgccg tgaacccgag gccgatggct ccaccggcag gatcactggc
    12781 cgcttcacca accgggtcac gagcaccagg gtcaggccga aaacgaccgc cgggacccac
    12841 cgtatgagct cgtacggctt catcatctgc cccagcgatc ccacgctcgg gacgacgctt
    12901 ccggcgacgc ccacgccgcc tttcgcgagt agccacccgg tccctgcgag gaaccccccc
    12961 acgaccgggt agggcacgta ccgcaccagg ttgcccagcc tgaacgtgcc cagcaggaag
    13021 aaggtgaggc ccgtcaacag ggtcaccacg agcgttgccg ccaccacgct caggaaggcc
    13081 cgatcggggc tgccgaacgc tccaaccgcc gtgctggtgg caacgatcgc gaggacggcc
    13141 gccgcggcgt cctgcacact cccgacgacg ccgcgggcac cagccctcca cgcgaggatg
    13201 gcaagcgtca acgccgccgc aagtaggtac agcccgatgc cgttggcgag gaacgcgacc
    13261 aggtatccac cgaagacgag ggcggcgaag gagaccgcga gtaccacctc gacggcaccg
    13321 atcacggccc cagccgtgat cacggcgagc gcgccctcgg atgatgggtg ctcggacacg
    13381 tcgccctccc tgcaacgtgg ctggcggaga actctatccg tacctccggg aacacgccac
    13441 agggcgccgt caacggcggc aggatcgcgc ttaaccgggg atcgccgagc ggaacccccg
    13501 agccagctcc agggcgccgt cccggtcccc ctcgaccaga cggcggacca gcgcgctgcc
    13561 cacgatgacg ccatctgcga aggcgcaggc ctccgcggcc tgtgcggggg tgccgatccc
    13621 tacgccgacc agcaggggac ggtcggtgag cggccggagg gatgccacga gctccctcgc
    13681 cgtgcccgcc aaccgctccc gctctccggt caccccgtac gtggccacgc agtacacgaa
    13741 gccccgagag cactcggcga tccggcggag ccggtcggac gagctcccgg gcgcggccag
    13801 gaagaccggt gcgacgtgcg cagccgcgca cagcccgacc caggcatcgg cctcgtccac
    13861 cggaaggtcg gggacgatca tggccgagac gccggctgcg gacgcgtcgg ccaggaaccg
    13921 atcggggccg tggcgcagga tcgggttcgc gtacgtcatg agcgcgaccg ggatcgagag
    13981 cccggcctcg cccaccgttg tcaggacatc ggtaggacgg gtccccgcct ccagggcacg
    14041 ccgcgaggcc tcctggatca ccccgccgtc catcaccggg tccgagaagg gaagaccgac
    14101 ctcgagggcg tccgcaccga tctcctgaag ggcgcggagc agatccgcgt ccaccccctc
    14161 cagtcccccg gtcacgtagg ggacgaagga ccggccgccg ccggccaggc ggtcccgcag
    14221 ggccgcctcc aggtcagcca accagcacct ccccaagcgc ggcacgaacc gagtccaggt
    14281 ccttgtcccc gcggccggac aggttgagca ggaccgagga tccctcgggc agggggcggt
    14341 ccaggagcca gcccaccgcg tgcgacggct ccagcgccgg gaggatccct tccagctcgg
    14401 ccagggcctg gaacccttcc agggcctggg cgtcggtggc cgagacgtac tcggccagcc
    14461 cggcctcctt caggtaggcg tgctcggggc ctacccccgg gtagtccagg ccggcggaga
    14521 tggagtgcgt ggggacgacc tggccgtccg catcctgcag caggtaggac atggatccgt
    14581 ggagcacgcc cggctccccc agcgtgagcg atcggcagtg gtccccgggg ccctcccctc
    14641 ggccgcccgc ctccaccccg atcaggcgaa cgccggcggc ccccacgtag ggggcgaaga
    14701 tccccatggc gttgctgccg ccccccacgc aggccaggac cacctgggga tcgccacctt
    14761 cggcttgtcg gaactggacc cgggcctcgt ccccgatcac ccgctgcagg tctcggacca
    14821 acgccgggaa cggatggggg ccggccacgg acccgatcac gtagtgcgta tccgagaccg
    14881 ttgcggccca gtcccgcatg gcctcgttca tagcgtcctt cagggtccgg ctgcccgagc
    14941 tcaccgggac cacctccgct cccagcagct gcatccgaga gacgttcggc gcctggcgga
    15001 cggtgtcctc ctcgcccatg tagaccacgc acggcagacc gaagagcgcg caggccgtcg
    15061 ccgtggccac cccgtgctgt cccgccccgg tctccgcgat gatcctgcgc ttaccgaggc
    15121 gcgttgcgag cagcacctgg cccacggtgt tgttgatctt gtgtgccccc gtgtggttga
    15181 ggtcctcccg cttcaggtag acccggtacc cgagccgatc cgacagccgc gcggcgaagg
    15241 tgagcggcga gggacgtccg acgtagtcgc gcaggagggc cccgagcggc ccccggaacg
    15301 agggatcgtc cttgagctcg gcccacgcgg ccgcgagctc atccagcgcc gggatcagga
    15361 tctcgggcac gtaacgaccg ccgaaccgcc cgaaccgtcc ccgggcgtcg ggcaggacca
    15421 ccggagcccc actcacgccg gagctcctcg gagcgctcgg agggcccgct cgggatcgcc
    15481 ggagcgcatg agcgcctctc ccaccagcac ggccccggcg ccggcgtccg cggcgcgccg
    15541 gacccgatcg ggggaaccga tcccgctctc caggaccgcg atccgatcct ccggcacctg
    15601 gcggagcaga ccgatggccc gatccaggtc gacgtccagg gtctccaggt cccgggcgtt
    15661 caccccaacg acctcggccc ccgaggtcac gacggcctcc agatcctgct ccgtgtgcgc
    15721 ctcggccagc acgtccagac ctagatcggc cgccgccgcc agcatggcga ccagctctga
    15781 ccgctccagg gcggcagcga tcagcagcac cgcgtcggcg ccctccgccc gggactggat
    15841 cagctgatct gggtggatca ggaagtcctt gcgtagcacc gggatcgagg tggccatccg
    15901 gaccgcgcgg aggtcgacga gagagccgcc gaagtgccga ggctcggtca gcacggacac
    15961 ggccgcggcc ccacctcgct cgtagccggc agcctgtacc cccggatcgg catcgccgat
    16021 gagcccggcg gacggggacg agcgcttcag ctccgcgatc accgccggtg cctccgaggc
    16081 tcgcagcgac cgggcgaacc ctcgggccgg cggcaggagg gcgatccgcc ccatgagcgg
    16141 ccccacgtcg atcggtcgcc ggtccaggtc ccggcgcacg tcccccagca ggtcggagag
    16201 gaagcccatg ggcccggaag tgtagcgggg gtcgcagcta tgatcgcgcc gctgggtccg
    16261 acgcgacctc ggactcaccc gcctccggtg aagacggctt cccgagcacg tcgcaggtcg
    16321 aggccctcgt acagcgcccg gccgatcacc gcgccctcca ggcccgcgtc cgcgaccgcg
    16381 cgcaggtcct ccggtcctcg aacgcccccc gccgcgatca ccggtcgccc ggtttcctcg
    16441 gccaccagac gcaggccgga cagatctggc cccgtcagct cacccacccg agccaccgcc
    16501 gtgcacagca gccggcgcac cggcaggcgg gccagccgct ccagcgtggc aggcagcggg
    16561 agccgaaccg cctcgccgcc tcgaggtcgg atcgatccgt cccggatctc gatccccact
    16621 accgcctcgg gtcccacggc gaccaagagc cgctcgagcc cgtggaggtc cgagagggcc
    16681 cccgagccga tcaccacccg agccgccccc gcctcgagcg ccccctcggc gtcgtcgagt
    16741 gagacgatcc cgccgctcgc ttgaacccga gcccccagac ctgccacccg gcggagcagc
    16801 gcgaggtcac gagggacacc ggtcaaggcg cgatccacgt ccaccaggtg gatccagacg
    16861 gcccccgcct cgaggaacgc cgcggccgcg gccagcgggt ccccgtggaa cgcgtccacg
    16921 ggttcgggtc cggccgggcc catccgggtc agccgaccgt ccgagacgtc cacggcgggg
    16981 atcacctcga accccacacg cctcacccta atggggcgga gggcagatcg taggagactg
    17041 cctcccgagt cgccgacagg agagcaccga agagccccgg gctggagccc tacgtctaga
    17101 gcgtgcccct gtcggtcgct ggggaggcgg agggctggtg gggtgtcgtg ccgagagcac
    17161 tgatccattc gctgcatgtc tcctcgggct ccccggcgat ccttccccgg aaaggagagc
    17221 aggaggtgat cttcctcggg atcgactggg ccgaggccca ccacgacgtc tgcctgctga
    17281 acgagggcgg gcgggtcctg gcgagcggac gggtgcccga tggtgtggac ggcgtcgcgc
    17341 ggctgcagga gctcgtggcc cagcatgcca gtgatgcaga cgaggtcatt gtgggcatcg
    17401 agctcgatcg cggcctgctg gtgggtgcgc tgctcgcctc gggctaccag ctctacgcca
    17461 tgaacccgct gtgggtcgac cgctaccgcg accggcaccg gacctccggc gccaagtcgg
    17521 accccggcga tgcgaaggtg ctcgccgaca tcgtgcggac cgaccggcac aaccaccgcc
    17581 cggtcgcggg ggacaccgag ggggctgagg cgatcaaggt gctcgccagg gcccacaagg
    17641 acctgaatct ggtcgcgcca gcgccagacc aaccggctgc gcaacctgct gcgggagttc
    17701 tatccccagg cgcttgcctg cttcggctcc gagctcgcct ccgccgacgc gctggccgtg
    17761 ctggagcggg cgccgtcgcc cgagcggggg cggcgactca cggtgtctca gatcctggcg
    17821 aggcttcgcc gaggcggccg gaggcggaac ctcaagctgc gtggggagga gatctccgcg
    17881 gccctgcgca gcccccagct cgaggcgccc ccggtcctga ccggcgccta cgccgcccgc
    17941 gtccacgcgc tcgtggcggt gctgcaagag atggcggcgc agatcgagag cctggagcag
    18001 gagctccgcc gatcgtttag gagccacccg gacgccgaga tctacctcag tctccccgga
    18061 ctcggcgatg tgcccgggcc ctcggcgagt tcggcgatga tccgacccgc tacgcgatcc
    18121 taggtcacgc aaggcccacg cgggaacggc cccgatcacc aaggcatcgg ggaccaagcg
    18181 cgttgtgctc gcccggttcg cccgcaacac ccacctcgcc gatgcctgcc acctgtgggc
    18241 gttctcggct gtcgcgctcg ccgggcgccc ggcgctatta cggcgccctc cgggctcgtg
    18301 gcaagacgca ccaccaggcc tcaggcagct cggcaaccgc ctcgtcggga tcctccacac
    18361 ctgcctggaa cgccgagagc tctaccgcga ggaagtcgcc tggcccgctc tcgaggaggt
    18421 tgccgcttga cagacacggg gcgtgggatg tctaggcggt cgcgggccgg gtggactcgg
    18481 acaccaccca gcggacggcg gtccggatcg cctccaccgc cgagggatcg aacgcgtggc
    18541 ccatctccgg caccaggatc agccgggctc gcggcgccgc cgaggccagc cgcaccgcct
    18601 cccgaggggc gacgaaccgg tcccggccgc cgtggaccac ggccagcggc acgaccaacc
    18661 ggacggccag gaccccgggc ggctccgggt cgagccactc tcgcgccagc cgcacgtgca
    18721 tctggtgccg cgcgaaccag cgtcccgccc gggtccgggt catcccggtg gcggcgaggg
    18781 cccgcgcgtt cctggggagg cgccacacgg cgggacagct caccgagacc aggccgtcca
    18841 ggagggggtc ggagacgccg tagcgcagtg cggcgatcgc ccccatcgag gcgcccaccg
    18901 gcaccacatg gtcggagcgc tcccgggccc acgccaccgc ggccccgacg tccagccgct
    18961 cccgatcgcc gagcgtgcag agaccgccgg agtcctggtg ccctcgcgcg tcgtaggaga
    19021 ggacgtcgta gccgtcggcc gccagcgcct tggccacggc caccagccgg gggtcgtcct
    19081 tggacgagct cagcccgtga gccaggatca cgctggtccc gagggatcgg ggcggctccc
    19141 atcggcgggc gcgcaggacg accccatcct gggtgaccag ggtggaaggc tcggacaccg
    19201 ctggggttgc cggccgcagc tcgttcacgc ccgggatccg accggccgtt tcggggccag
    19261 caatccgtgc cggcgcagcg tcccccggaa ccagaacgag gacagggcca cggcccccac
    19321 cgtgaacacc gccgtggacg ccgtcttggc catcacgaag acaccgaccg gctcggtcag
    19381 gaggaaccac agcgtgacgg ccgcgttgct cagcaggacg aaggcccaga gcagcgtgat
    19441 gcgcaggaac accttgcggg ctaccgggtg cgaggccagg gcgtggggca gcggcacgaa
    19501 gtcaccggcc agccgctcgg cgagcgggcg tcccaccggc accgacagga ggaaggccag
    19561 cccgatggcc actgtcccca gggtgggctg gaggaagtac acgaacacgc tgccggtgag
    19621 gaaggagatc agcgtccgaa cggtgatcgc ggcggtcccc aggagcagca gccccgggac
    19681 ccgcttgccg aggatcagac ggacggccaa cgccgcatag gaccaggcca gcgcgccgat
    19741 caacgctccc cacacgccga cgagccacag cgccaggtag aagatcgcga gcggcatcag
    19801 ggtgccctcg accacgtgcg ggatcgcgtg gcgtgcgagc gcggacaaac gcgggatctg
    19861 gaatgaacct tgcacttgat cgatcggtgg gccggtcggg cccaagagcg atggtatcgg
    19921 atccggcgag gcttcgcccg acccccgggc cggtcggccg cctacaatcg tcgcgtgcag
    19981 acctcggggc ggctcgggcc ggcggacctg cgggccgcct tcgtcacgta ccgcgagatc
    20041 ctccatgcgc accgccaggt gatcaaccgg ttgaacgtgt tccccgtgcc cgacggggac
    20101 acggggacga acctctcctt gaccgtcgac gccgtggtgg ccgaacttga gtccgacgcg
    20161 ggcatggagg cgaccgctcg ggccatcggc cacggcgccg tgatgggcgc ccgggggatc
    20221 tcgggagtga tcgccgccca gctgttccgg agcgccgtgg atcacctggc ggtcgccccg
    20281 gaggtcgatg gagcctcgct ggccgcggcg ctcgatgcgg cggcccgggc tgcctacggg
    20341 gcggtctcca ggccggtgga gggcacgatc ctgaccgtgg ctcgggaggc ggccgcggcg
    20401 gccgggtcgg cggcgggaga gggggatctg ctgacggtga tccaggcggc ccgccgggcg
    20461 gcttgggagg ccgtggagcg gacgcccgac ctgctgccgg tgctccgcga cgcgggggtg
    20521 gtggacggag gggggaccgg cttcgccctg ttcttcgacg cgctgctcca cgtggtggcg
    20581 ggcacgccgc tgcccgaccc ttccgaccga gcggccgacc cggtgctgac cgcctggtcc
    20641 cggtggagct cggcacccga tggggctgga gacggggcgg tctccgctca cccttcggac
    20701 gcgcgccccc ccggcgggga ccatggcgat cggcgcttcg aggtcgcctt gctgctggag
    20761 gcgccggacg aagccgtacc ggccttccgc gacgtgtgga accggctggg cggggcggtg
    20821 gtcgtggtgg gcggcggcgg ccggtggagc gcgcacgtgc acaccgacca ccccggcagc
    20881 gccgtggacg cggccaagag cttcgggcgt cccagcgacg tccgcgtgac cgatctcgcc
    20941 ctccagatcg tcgaggagcg cggcgacctg gcggcgccgc aggagcggac ccggtgtggg
    21001 gcggtggcgg tgggggccgg cgatgggatc catcgcctgt tccgctcgct gggggcccgg
    21061 gtggtgaccg gcatcggctc gccgaacccg tcggtgcgcg agctggtcga ggccgtggag
    21121 tccgttgcct ccgacgaggt ggtggtgctg ccgaacgacg atgacgtggc gcccgcggcc
    21181 gagcaggtcg ctcggctctc ggggaagcgg gtggtggtgg tgcccacccg ggggatcgct
    21241 cacggcctgg ccgccctgcg cgccttcgat ccggatgccc ctgccgagga gaacgccgag
    21301 gccatgtcgg aggccgcgcg ctcggtgagc ttcgggcgtc tggtccgggc ggttcgggac
    21361 gcgggaagcg aggtcggcga catccacgag ggcgactggc tgggcatcac ggccgagggg
    21421 atccgggtgg tcgagcccga ggtccggcag gcgggacggg ccctgctcga gcggttgctc
    21481 tcggaccgct ccgagatcgt gacgatcatc gagggggagg gggccaccga ggaggtgacc
    21541 cgcgatctca cggactggct gcaggcgatc cgtcccgaga tcggggtgca ggtgctccac
    21601 gggggacagc cgatccacgc gtacctgttc ggcctggagt gatccacgcc gggaccgcgg
    21661 aggccggctc cgctacggac ccaggttgta gccgagccgc ctggggaggt gggccagggc
    21721 ccgggtgatc gggcccgggc gccgtccgtc gtcctccagg tcgtccttgc gcagcaccgc
    21781 cgcggtcacc agccgctcgc ccggcacgaa caccgggtcc ggcgggaacg ccttcggccg
    21841 gtcgtcgacg atcggcagcg acgcgagggc gtctggccgc tcggcggcga tcgaggcgag
    21901 gatccggccc gccagccggg acggtcccac gccgttgccc gtgaagccca gcgcgaagtg
    21961 cgagcgccca tcgggcaggg tgccgaggaa cggcacgtgg cggccggaga cgtcgatggg
    22021 accgccccag gcggcctcga ggggcacggt ccggaagggc gggaaccacc ggtggaggtc
    22081 ctcaaccagg cggcggatcg agcgctcctc gtagaggaag cggggcccga tccgacggcg
    22141 cgaggccacc tgcatgccgg cgcccccgaa ggcgatccgc ccgtcgggcg tggtccgcag
    22201 gtagtgcagc gcggtgcgga agtcgtagag gccctcgccg ccggtccagc cgagctcggc
    22261 caggcgctcc ggtgccggct cggtgatcac gatgaacgtc ccccggacca tgaccgcccg
    22321 tcggaaggcc cgccagtgca tgccccaggc gttgacggcc aggacggcgt gcccggcggt
    22381 caccgtgccg cccggggttt cagccacgat gcgcggtccg gcgtggaacc ggcggaccgg
    22441 cgtgccctcg tggatccgga ccccccggtc gagcagcacc cggcgcaggc cacgcgccag
    22501 ccgggccggc tggaccttcg ccgcgtcggg gatccacacc gcgccccgga gcagcggcga
    22561 ggcgcagtgg gcccgcgcct cgtcggcgct cagccgttgg tacagctcgc cgatccccgt
    22621 ctcggccgcc tcgcggatca ccgagtccag caccgcgtcc tgagccgggg aggccgagat
    22681 gcccaggtcg ccgtccttcg cgtaccaggc gtccacccgg tgctcctggc accaggcgcc
    22741 gatcccgtcg atctcctgcc cggcggcccg agccacggcc atgccgccct ccggcccgta
    22801 gcgctcgatc agcagaccca ggtcgtccca caggccgttc acgaagcccc cgttgcggcc
    22861 cgaggggccg cccccgcaga tgtcctgctc gagcaggacc acatcgatcc cggggtccac
    22921 ctgcttcagg aactccgcgg cccacatccc cgtgtagcca ccacccacga ccaccacgtc
    22981 ggccgaggtg tcgccggaca gcggcgggca cggctcgcca gggtcgtggg ccagcgcctc
    23041 ctccatccac cagctgcgga cggccgcccg cgggaccccg ccgctcatcg gggccccagg
    23101 ttgtagccga gccgccgagg gatcctggcc acgaaggtcg tgagcggacc ggcccggcgc
    23161 ccctcggcct cggcgtcgtc cttgcgccgg atcgcctcgt tcaccatcag catgccgggg
    23221 gacagcagcg gctcgggcgg gaggcggagt ggctcgttcg tgaccagggc cagccgggag
    23281 aacccgtcct cggcgtgcag ggcgaggctc gccaggatct gccccccgag gtgcgcgggt
    23341 gccacgccgt tgcccgtgta tcccaggccg tagtgcacgg tgcccgaagg gaagctgccg
    23401 aagaacggca ggtacagccc ggccacgtcg atcggtcccc cccaaccggc ctcgatcggc
    23461 acgtcgcgga agctcgggaa catccggtgc aggtccccaa ccgccctgcg caggaacccg
    23521 acgtcgtacg cgaacctggg gccgatccgc cgggacaggc cgggctgcat ccccgcgcac
    23581 ccgaaggcga tccgcccgtc gggcgtggtg cgcaggtagt gcaacgaggg gcggaagtcc
    23641 cggatgccct gcccttcggt ccagttgatc tcttcgagcc tggccggtgc cggcgccgtc
    23701 aggaccatgt agctgccccg cacccccacg tacgggtgca ggcgcttcca gtggatcccc
    23761 caggcgttca cggccagcac gccctcgccc gcgcgaacgg tgccgccggg ggtctccgcc
    23821 acgaccggtc ggccggcccc gaagcctcgg acgggcgtct gctcgaagat ccgcaccccg
    23881 cgttccagca ccacccggcg cagaccccgc gccagccgag cgggctggac cgtggagaac
    23941 tccttggaca ggaccccgcc cccgaacatc ggtgagatgc agatgtcggt gacctcggac
    24001 ggcgtgagct cctcgaactc gtcctctacg ccgtgatgcc gcgcttcctc cagcatcgcc
    24061 gtccatgccc cctcgtggaa gggggatgac gccaccgcca gctcgccgtt cgggctccac
    24121 cacgcatcga tgtcgtgacg gcgcaggaag tcgccgaccc cccgcacgct ccgggaggcc
    24181 gccaggcaga gctccatcgc gtcggcatcc ccgaagcggc gggccatctc gccgatcgac
    24241 gtccaccagc cgttcacgaa cccaccgttg cgaccggagg gcccgcctcc gcagatgtcc
    24301 tgctcgagca ccaccacctc gatcccgggg tcgcgttcct tcaggaagta cgcggtccac
    24361 agtcccgtgt agccgccgcc cacgatcacc acgtcggcgg cgaggtcctc cgccaggggc
    24421 gggcacggct cgccggcgaa cgccggttgg gccagggcct cctgcagcca ccagctgcgg
    24481 tcggcccggg gaggcgcatc cgttctcgtg gagttcatcc gaggctccct ggtatcgtgc
    24541 gggtccattc tgtctcaccg ttcaggaggt cgacgtgggg gccgacaaca ggccgcggag
    24601 ccggcaggtg accgagggac cggaacgagc ccccgcccgc gcgatgctcc gggcggtcgg
    24661 cttctccgag gaggacttcg gcaagccgca gatcggcgtg gcgtcgtcct ggaacgaggt
    24721 gaccccctgc aactaccacc tcgacaagct ggccgcgctg gccaaggagg gcgtccgaca
    24781 gggggacgcg gtgcccatcg agttcaccac gatcgccgtc tccgacggca tcgccatggg
    24841 ccacgagggc atgaaggcct ccctggtcag ccgtgaggtg atcgccgact cggtggagct
    24901 ggtgatgcac gccgagcgca tggacggcct ggtaggcatc gccggttgcg acaagagcga
    24961 gcccggcatg ctgatggcca tggcccggct gaacctgccc gccgtgtacc tgtacggggg
    25021 gacgatcctg ccggggacgt accggggccg cgacatcacg atccaggatg tgttcgaggc
    25081 cgtgggggcc aacgcgaggg gcgggatgtc ggacgaggag ctgctggccg tggagcgggc
    25141 cgcctgtccg accaccgggt cctgtgccgg catgtatacg gccaacacga tggccgccgc
    25201 cgccgaggcc ctggggatgt cgctcccggg ggcggcctcg ccgcccgcgg tggactaccg
    25261 gcgcgaggtc ttcgctcggg ggtccggcgt ggctgccgcg aagctgctgg agctcgggat
    25321 ccggccgcgc gacatcatga ccaagcccgc cctcgagaac gcgatcgccg tggtgatggc
    25381 gctggccggc tcaacgaacg cggtgctgca cctgatcgcg atcggccacg aggccggtgt
    25441 tgatctgagg ctggacgact tcgaccggat ctcacgccag gtcccgcacc tggtggatgt
    25501 ccggccggcc ggacggttcg tgatgagcga tctggaccga gcaggcggcg tgcccgtggt
    25561 gatgaaggag ctgctcgagg ccgggatgct ccacggcgac tgcctcaccg tgacgggtga
    25621 gaccgtggcc gagaacctcg cggcgatgga gatcccggag ccggacggcg acgtggtgcg
    25681 gccggtctcc gagccgatcc acgccgaggg cggcacggcg atcctccacg gctcgctcgc
    25741 ccccgagggc tcggtgatga agatcgccgg ggccgagggc ttggacttcc ggggcaccgc
    25801 gcggcccttc gactccgagc aggccgcgtt cgacgccctc acctcgggga ggctgaaggc
    25861 gggggacgtc atcgtgatcc ggtacgaggg gccgaggggc tcgcccggca tgcccgagat
    25921 gctcgccgtg accgcggcgg tcgcgggcgc gggtctcggc gccgacctcg cgctgatcac
    25981 cgatggccgg ttcagcggcg cgacgaaggg ttacagcgtg ggccatgttg cgcccgaagc
    26041 cttcgtgggc ggaccgatcg cgctcgtccg agacggcgac cagatcgtga tcgatgcggc
    26101 gaaccgccgg ctggacctgc tggtcgagga ggccgagctc gaccgccgcc gcgccgcatg
    26161 gagcgctccc gagccccggt acgcgaccgg cgccctggcc aagtacgcgc ggctggtggg
    26221 ctcggcgtcg cggggggcgg tcacctcgtg acgaggctca cggaaccccc ggtcctcgag
    26281 tgccgggagt gcggcgcgcg ctacccggtg gagcccctta cgatctgcga ggagtgcttc
    26341 gggccgctcg agccgtggta cgacctgtcc gcgatcgacg gcacggactt ccgcaaccac
    26401 gcggaggccg gcccacccac gctgtggcgg tacgagatgc tgctgcccgg cggcccaggc
    26461 gtgcggaggg tggacctggg ggccggcttc accccgatgc ggcgtgccga gcgcctcggt
    26521 gagcgcctgg ggatcgagaa gctctggatc aaggacgact cgggcaaccc gtcgaactcg
    26581 ttcaaggatc gggtggtctc ggtcgccatc acggtggctc gggcgttcgg cttcgaggcg
    26641 atcagctgcg cctccaccgg gaacctggcc aacgccaccg ccgcgcacgc cgcgaaggcg
    26701 gggatgcccg cctacgtgtt cgtgcccgac gacctggagg tggccaaagt cctggccacc
    26761 gaggcctacg gggccaaggt ggtggcggtc gacggcagct acgacgacgt gaaccgcctg
    26821 tgctccgagg tggccgaggc cctgccctgg gcgttcgtga acgtgaacat gcggccgttc
    26881 tacacgcagg gctcgaagac cctggggttc gagaccgccg agcaactggg ctggcggctg
    26941 cccgaccacg tggtggtgcc gatcgcctcg ggtgccctgc tcaccaaggt ccatacggcc
    27001 ttccaggagc tgatcgcgat cggggccgtc gaggagacgc cctaccggat cagcggggct
    27061 cagcccgagg gctgctcgcc ggtggcccag gccttcgccg cgggcaccga ggaggtgaag
    27121 ccggtcaagc cgaacacgat cgcccgctcc ctcgggatcg gcaacccggc cgacggctac
    27181 tacgccatcc ggatcgcgaa ccacaccggt ggggcgatcg acgcctgcgc cgaggacgag
    27241 gtgatcgagg gcatccgtct gctcgccgag accgagggcg tgttcaccga gacggccggc
    27301 ggagtgacga tcgcggtgct aaagcgtctg cgcgaggccg gcgtgatcga ggccgaccac
    27361 gagacggtcg ccctcatcac cggcaacggg tacaagacga tcgaggcgct gcagggcacc
    27421 ctcgagccta cctaccacgt cgagcccgac ctcgacgcat tcctggccgc ccttggcccc
    27481 tgatccgccc gatggcgctg gtccatcctg accatgcccc aacggtccga gagcgagacg
    27541 gggaaggctc ccggcctcgg cgctcgcgta ccctgggcga gccatggcgg acgtgctgcg
    27601 tgtgcagggg gcggaggttc ggatcggcgg acggcggatc ctggggccgc tatcgctgtg
    27661 tatcgatccg ggcgagcggt gggtggtcct cggctcgaac ggctcgggca agacgaccct
    27721 gctgcgactg gccggcgcgc tccggcagcc gagcgccggc accgtcgagg tcctgggcga
    27781 acgcctcggg gccaccgacg tccgagcgct ccgcgcccgg atcgggttcg tggggcacgc
    27841 ggtggccgac gagctcccgc cgggactttc ggtggtcgac gccgtcctca ccggcaagcg
    27901 tgccaccctg gtgacctgga tgcagcagct cggccacgcc gatcggcggc gagccgcggt
    27961 gctgctcgag cacgtggggt gcgcgcagct ggaggggcaa ccgttcaggc agtgcagcca
    28021 gggcgagcgc cagcgggtcc tgctggcccg ggcgctgttc ggtaaccccg aactgctcct
    28081 gctggacgag ccggcggccg ggttggacct gccggggcgc gagtccctga tccgagcgct
    28141 cgaagcggcc gacgatctgg gctcgacccc cagcgtcctg gtcacgcacc acctcgagga
    28201 gatcccgccc accaccacgc acgcggcgct tcttcgggcc ggcgaggtcg tggttcaggg
    28261 gccggtggac gaggtcctcc tcgacgagcc cgtgagcgcc tgcttcgatc tgcccgtccg
    28321 ggtcggccgc aagggctcgc ggtggtcggc ggtgtccctc gaacggccta ccggacatct
    28381 ggcctgaacg ggcgggttga caggtcgccg atggcctcta gactgatgtg accatgtcga
    28441 cgagcgagac gttctccgtt tccgggaagc agtcgctcgt acttattacc taggcgcggc
    28501 caggcccagg gaggcctgcg tcgcgccgga tgccctccgc ggatgcgggg ggttttttct
    28561 acccccgatc cagggccgcc ggccgcacgg gaaccccggg ccacgcgagg aaggagcaga
    28621 ccatgaggta cgagttcgag cgggacggcc gcacggtggc cagcgtcctg tgggagggcc
    28681 cggcacaggt cacggtcgac gtggccgacc cggccgaccg ccctcagttc gaccggttct
    28741 tcggagccga ggtcgtgtac atggacgcgg gcttcgactt cgggggcggg atgagcgaga
    28801 gcggtctgac cagccgccgg cgcgactgga ccccgtggga gttcgagcgc gcctgtcgca
    28861 atctggccca caagctcggg tgcacggtcc accgggtgga gacggggccg gtcgaggccc
    28921 gacccgagga ggtcgccagc cgatgagggt gaccggcgcc caggctctgt tcaaggccct
    28981 ggagggcgag ggggtcgacc tggtcttcgg gatccccggc ggagccatcc tgcccgccta
    29041 cgacccgctg atcgattcga gcatccgcca cgtcctgacc cgccacgagc agggcgccgg
    29101 ccacgccgcc gaggggtacg cctgggccac cgggaaggtc ggcgtatgca tcgccacgtc
    29161 cgggcccggt ggctgcaacc tggtgacgcc tctcgccgac gcgaagatgg actcggttcc
    29221 gctggtggcc atcacgggcc aggtgaactc cgccgcggtc ggcaacgacg ccttccaaga
    29281 ggcggacatc accggcatca cgatgccggt caccaagcac aacttcctgg tgaaggaccc
    29341 tggcgacgtc gccgagacca tccgggaggc cttccacatc gcctccacgg ggcgccccgg
    29401 cccggtcctg gttgacctgc ccaaggacat cctgatcgaa gagaccgagt ggacctggcc
    29461 cgagcgtgtg gaactgccgg gctacaagcc caccaccaag ggcaacctca agcaggtccg
    29521 cgaggcggtc cgatcgatca tggcggcgga gcgcccggtc ctgtacgtgg gcggcggcgt
    29581 gatcaaggcg aacgccacga aggagctgtt cgagctggcc accgagggca acctccccgt
    29641 ggtcaccacg ctcatggccc gcggtgcgtt ccccgacgac cacgagctgg ccttcgggat
    29701 gccggggatg cacggaggct acacggccgt cacggcccta cagaagaccg atctcctggt
    29761 ggccctggcc tcgcggttcg acgatcgggt gaccgggcag ctgtccacgt tcgcgccgaa
    29821 cgccaaggtg atccacctgg atatcgaccc ggccgagatc ggcaagaacc gccgcgccga
    29881 cgtgccgatc gtgggggacg cccgggatgt gatctccaag ctggtggcgg agctccggcg
    29941 gcgccaggag gaggccggcg gccctccgga ccgctccggg tggctcggcg tgctccgctc
    30001 gtggatggag cgttacccct acacctacga gcagccggac gatgggccac tgaagccgca
    30061 gttcgcgatc caacgcctct acgaacacgc acgggatgcc atcgtggtgg ccggcgtggg
    30121 ccagcaccag atgtgggcct cgcagttctg gcggttcaac gagccgagga cgtggatcaa
    30181 ctctggcggg ctcgggacca tggggttcgc cgtgccggcc gccctgggtg ccgcggccgg
    30241 ccggccggac cggcgcgtga tcgcgatcga cggggacggc tgcttccaga tgactgccca
    30301 ggagctggcc acgtccagca ccgaggggat cccgttcgtc acggcgatca tgaacaacgg
    30361 ctacctcggc atggtccggc aatggcagga gctcttctac cgagagcgct actcgcaggt
    30421 ggagctcacg cagaccaccc ccgactacgt gaggctggcc gaggcctacg gggcggtcgg
    30481 cctccgcgcc gagcacccgg acgaggtgga cccggtgatc gagaaggccc tgtccgtcga
    30541 ggaccgcagc gtggtgatcg acttccgctg tgaccccgga gagatggtct tcccgatggt
    30601 cccggccggg gccagcaacg acgacatcat cgtgggtccc ggcggcctcc ggccggtgga
    30661 cgaccccgcg gaggctccca tcgcatgaac ggcaacggat ccctccacac catctcggtc
    30721 ctggtcgagg acaggccggg cgtcctgacc cgggtggccg ggctgttcgc ggcacgcgga
    30781 ttcaacatcg actcgctggc cgtgggtccc accgaggagc gcggtctgtc acgcatgacc
    30841 ctggtggtga acgtcgagag caaaccgctc gagcaggtcg gc
//
LOCUS       DASQQQ010000228         6529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_93925, whole genome shotgun sequence.
ACCESSION   DASQQQ010000228 DASQQQ010000000
VERSION     DASQQQ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6529
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93925"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1091
                     /locus_tag="VE646_08850"
     CDS             150..1091
                     /locus_tag="VE646_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HZD80133.1"
                     /translation="MYRTMLQARLCDEASFRLNRQGKAPFVVPVSGHEGCQVGTAWPL
                     QRGRDIWLPYYRDLAVVLAAGMTPKDIFLGLFAKADDPSSGGRQMPSHWGSRRLNIIS
                     HSSPIGTQIPHAAGIAYAVRYRNEDAVVACWFGEGATSEGDWHEGLNFSGIHKLPVIW
                     VCENNQYAISVVQRKQMAVQDVADRAAGYGFPGVVVDGNDVLACYRAMEEAVQRARAG
                     EGPTLIEAKTYRFQPHTSDDDDRTYRTREEVEAAKVKDPLVIFASYLKERRLLDDAGI
                     ESLTAEVKAEIDRAIDDAWNAPDPDPSTLGHHLFSEP"
     gene            1093..2079
                     /locus_tag="VE646_08855"
     CDS             1093..2079
                     /locus_tag="VE646_08855"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018672510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZD80134.1"
                     /translation="MAEKNVVTAIHDALHEAMAADERVVVLGEDVAARGGVFRITQGF
                     LDEFGERRVIDAPLAESGIVGVAVGMALHGLLPVAEIEFADFIHPAFDQLVSEVARMR
                     YRSNDDFHLPMVVRAPWGGGIHGALYHSQSIEAFYGHVPGLKVVVPSTPADAAGLLRA
                     AIEDPDPVMFLEHKKTYRLISGEVPEDPFTVPIGKADVKRGGDTLTVIAYGLMLHQCL
                     EAAQRLQEEQGMSAEVVDVRTIAPLDKETILASVRKTGKAMVVYEDNRTYGAGAEIVA
                     TIAEEAMFDLDGPLVRIGGPDLPAMGFAAPLEHAFMLDTDRIYDQMVELARF"
     gene            2115..3098
                     /gene="lipA"
                     /locus_tag="VE646_08860"
     CDS             2115..3098
                     /gene="lipA"
                     /locus_tag="VE646_08860"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HZD80135.1"
                     /translation="MASSHNPQGRRLYLPIVDAGASGARAAAPGVNRPGSRKPPWLKA
                     RLPGGENYRELKGLMRGLSLHTVCEEASCPNIGECWEEREATFLILGDRCTRRCGFCD
                     VMTARPEPVDEDEPARIGEAVARLGLRYVVLTGVARDDLRDGGARIWAATIRAIRSSL
                     PGCRVEVLPSDFKGGERDIAAVIEAEPDVFAHNLETVRRLHERIRPAFGYDRSLEVLR
                     IAKRLRPGQVTKSNLILGMGERPDEVPDAIRDLADADIQILTIGQYLQPTQMHLPVDR
                     WVAPEEFEEHANTGAALGIHHVEAGPLVRSSYHAGKQLRRALAATGAGLRS"
     gene            complement(3101..3492)
                     /gene="rnpB"
                     /locus_tag="VE646_08865"
     ncRNA           complement(3101..3492)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VE646_08865"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(3527..3988)
                     /locus_tag="VE646_08870"
     CDS             complement(3527..3988)
                     /locus_tag="VE646_08870"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HZD80136.1"
                     /translation="MATDGAARGNPGPAGIGVVVLTPEGEVLAEIARGLGVATNNVAE
                     YRAAIAGLRRAAELGGTDVLLRSDSHLLVEQLSGRYRVKNPRLQKLHAEARQVARGFR
                     QVRFEHVPRERNKHADRLANRGVDEWLAGEGAGSTRPDPPARLWEEDPPPG"
     gene            complement(3991..4701)
                     /locus_tag="VE646_08875"
     CDS             complement(3991..4701)
                     /locus_tag="VE646_08875"
                     /inference="COORDINATES: protein motif:HMM:NF018970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscO family type III secretion system apparatus
                     protein"
                     /protein_id="HZD80137.1"
                     /translation="MEGMDRILELQALDTTIDRLQARRRQLEEGEEVRVARSSMEQAE
                     GALGEIRLALDAVARDQQRLEHEIDSMTQKAAAEEKRLYDGSIANTKELEALQHEIAS
                     IRDRRSRTEDRLLDQMEQREALEDRAGAAETELGRAREQLEALGGSAAEELRSIEEDL
                     SRRSSEREGLLPAFDRELLDLYEEIRAQKKGVAVARLVDGVCQACHEQLSAMEIDRLK
                     RTQDVRRCEYCRRLLIVG"
     gene            complement(4764..>6529)
                     /locus_tag="VE646_08880"
     CDS             complement(4764..>6529)
                     /locus_tag="VE646_08880"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZD80138.1"
                     /translation="VYGTERRGCHRRYVTTEVLAQWESAKSPYRVTLQDGTELIASGD
                     HRFLSGRGWKHVTGAEQGRNPRPFLTPNDPLLGPGRFAPAPVHDEAYRRGYLTGVIKG
                     DASLGYHSYPRADRSPGTAHGFRLTLTDRDGLERTRRYLSLEGIETTPFPVHPGSERS
                     RHLHVIRTRRGGAIERIRWIITRPSAPSPSWSKGFLAGIFDAEGSCGRDAIRISNTDP
                     QVIGRVVSSLRALEFDAVIEDPHRENGLKSVRVRGGWRERLAFLQTVDPAIERQRSLE
                     GLVVKGDAPLRVAAVEPLGEELPMFDLTTGTGDFVADGVISHNCFARPTHTYLAMNAG
                     RDFERKIVVKVNAPEVLRRELAAKRWKGEQVAMGTNTDPYQRAEGRYRLMPGILRALT
                     EYRNPFSILTKGILIVRDLELLLAAAEVTEVAAAFSIGTLDEDAWRRTEPGTPHPRRR
                     IEAVRALNEAGIPCGVLMAPILPGISDDPRQLREVVRAALDAGATHVSPILLHLRPGV
                     REEFLPWLEETYPDLVPRYRDLYRKAYAPSADREALGRRVRGLVRALGGPRPAPQAPP
                     RWRRDADLEASGDGPRQLTIL"
BASE COUNT          936 a         2351 c         2263 g          979 t
ORIGIN      
        1 cccccccccc ccgcggagtg gcccgttgac cacccgagag ccgcttcggc cgatgctaga
       61 ctcgggcgca ggagggtgat gtggcgaccc gagtaacctc ggacacttcc cacgaggctc
      121 tgggcctcac cggcgacgac ctcctcgcca tgtaccgcac catgctccag gcccgtctct
      181 gcgacgaggc gtcgttccgg ttgaaccggc agggcaaggc gccgttcgtg gtcccggtct
      241 ccggccacga gggctgtcag gtggggaccg cgtggccgct gcagcggggc cgcgacatct
      301 ggctccccta ctaccgggac ctcgcggtgg tcctggccgc cggcatgacg cccaaggaca
      361 tcttcctggg gctgttcgcg aaggcggacg acccctcctc gggtggtcgg cagatgccct
      421 ctcactgggg atcgcgcagg ctgaacatca tctcccactc ctcccctatc gggacgcaga
      481 tcccgcacgc ggccgggatc gcgtacgcgg tcaggtaccg caacgaggac gccgtcgtgg
      541 cctgctggtt cggcgagggc gccaccagcg agggcgactg gcacgagggg ctgaacttct
      601 ccggcatcca caagctgccg gtgatctggg tctgcgagaa caaccagtac gccatctcgg
      661 tggtccagcg caagcagatg gccgtgcagg atgtggccga tcgtgccgcc ggctacgggt
      721 tccctggcgt ggtggtggac ggcaacgacg tcttggcctg ctaccgggcc atggaggagg
      781 cggtccagcg ggcccgggcc ggcgaaggtc ccaccctgat cgaagccaag acgtaccggt
      841 tccagcccca cacctccgac gacgacgatc gcacctaccg cacgcgcgag gaggtcgagg
      901 cggccaaggt caaggacccg ctggtcatct tcgcctcgta cctcaaggag cggaggctgc
      961 tcgatgacgc tgggatcgag tccctcaccg cggaggtgaa ggcggagatc gaccgcgcca
     1021 tcgacgatgc ctggaacgca cccgaccccg atccgagcac cctgggccac cacctgttct
     1081 ccgagccctg agatggccga gaagaacgtc gtcaccgcca tccacgacgc gctacacgag
     1141 gcgatggccg ccgacgaacg ggtcgtggtg ctgggagagg acgtggcggc acgaggaggg
     1201 gtcttccgga tcacgcaggg gttcctggac gagttcgggg agcggcgcgt gatcgacgct
     1261 ccgctggccg agtccgggat cgtgggcgtg gccgtgggca tggcgctgca cggcctgctg
     1321 cccgtggccg agatcgagtt cgccgacttc atccatcctg ccttcgatca gctggtgtcc
     1381 gaggtcgcga ggatgcgcta ccgctccaac gacgacttcc acctgccgat ggtcgtccgg
     1441 gccccctggg gtggagggat ccacggcgcc ctgtaccact ctcagtcgat cgaggccttc
     1501 tacgggcacg tcccgggact caaggtggtc gttccctcca cccctgccga tgccgccggg
     1561 ctcctccgcg cggcgatcga ggaccccgac ccggtgatgt tcctggaaca caagaagacc
     1621 taccgcctga tctccggcga ggttcccgag gacccgttca ccgtgcccat cgggaaggcg
     1681 gacgtgaagc gggggggcga cacgctcacc gtgatcgcct acggactgat gctccaccag
     1741 tgcctggagg cggcgcagcg gctgcaggag gaacagggga tgtcagccga ggtggtcgac
     1801 gtgcggacca tcgccccgct ggacaaggag acgatcctgg cctcggtgcg caagaccggg
     1861 aaggccatgg tggtctacga ggacaaccgc acctacggcg cgggcgccga gatcgtggcc
     1921 acgatcgccg aggaggcgat gttcgacctg gacgggccgt tggtgcgcat cgggggaccg
     1981 gacctgccgg ccatgggctt cgccgcccca ctggagcacg ccttcatgct cgacaccgac
     2041 cggatctacg accagatggt ggagctcgcc cgcttctgat cggcgatcgt cccaggacgg
     2101 atagactcgg aacgatggcg tcttcgcaca acccgcaggg ccgacggctg tacctgccca
     2161 tcgtcgacgc gggggctagc ggcgcgaggg cggccgcccc gggggtcaac cggccgggat
     2221 cccgcaagcc gccctggctg aaggccaggc tgcccggcgg ggagaactac cgcgagctca
     2281 agggcctgat gcgcgggctg tccctgcaca ccgtctgcga ggaggcctcg tgccccaaca
     2341 tcggcgagtg ctgggaggag cgcgaggcca ccttcctgat cctcggagac cgatgcaccc
     2401 ggcgctgtgg gttctgcgac gtgatgacgg ccagacccga accggtggac gaggacgagc
     2461 cggcccggat cggggaggcc gtggcccggc tggggctgcg gtacgtggtc ctgaccggcg
     2521 tggccaggga cgacctgcgg gacggtgggg cccggatctg ggcggcgacg atccgcgcga
     2581 tccggagctc gctccccggc tgccgggtgg aggtactgcc cagcgacttc aagggcgggg
     2641 agcgggacat cgccgccgtg atcgaggccg agcccgacgt gttcgcccac aacctcgaga
     2701 cggtccggcg gctccacgaa cggatccgcc cggcgttcgg ctacgatcgc tcgctggagg
     2761 tgctgcggat cgccaagcgc ctccgcccgg gacaggtcac gaagtcgaac ctgatcctgg
     2821 gcatggggga gcgccccgac gaggtgcccg acgcgatccg tgacctggcc gacgccgaca
     2881 tccagatcct cacgatcggc cagtacctgc agcccaccca gatgcatctg ccggtcgatc
     2941 gctgggtcgc gccggaggag ttcgaggagc acgcgaacac cggcgcggcg ctggggatcc
     3001 accacgtcga ggccggtccg ctggtgcgga gctcctacca tgcgggcaag cagctccgcc
     3061 gggcgctggc ggcgaccggt gccggcctgc gtagctgagg cggaggagcc ggcctgtaag
     3121 ccgggttctg tgcccgatct cgcggaccgg cggcgaccat ctctctgagc cgcgcatcgt
     3181 ggcgcgaccc cggcggcgcc cggtaccacg ggcgccgctc gtgcggccta cccgggagca
     3241 tcgggcgggc cgccctcgag cgctccctgc ttggccttgc tccgggtggg gcttgccgag
     3301 ccggaccggt cacccggccc gctggtgagc tcttacctca ccgtttcacc cttaccccct
     3361 ctcgggggcg gtctgctctc tgtggcgctg tccgcgggtc accccgcctg ggcgttaccc
     3421 agcaccctgc cctgcggagc ccggactttc ctcgaccggt ccgtgaccgg ccgcggccgc
     3481 ccggccggct cctccacctg cgagtatagg agctggggcc gacgcgtcag ccaggcggag
     3541 ggtcctcctc ccacagccga gcggggggat cgggacgcgt ggagccggcc ccctcccccg
     3601 ccagccactc gtccaccccc cggttggcca ggcgatccgc gtgcttgttc cgctccctgg
     3661 ggacgtgctc gaagcggacc tggcggaacc cccgagccac ctggcgagcc tcggcgtgga
     3721 gcttctgcag ccgagggttc ttgacccggt agcggccgga gagctgctcc acgagcagat
     3781 ggctgtccga gcgaagcagc acgtcggttc cccccagctc cgcggcccgc cggagacccg
     3841 cgatcgcggc cctgtactcg gcgacgttgt tcgtggccac gccgaggcct cgggcgatct
     3901 cggccaggac ctctccctct ggcgtgagca ccacgacgcc gatgccggcc ggaccggggt
     3961 tcccccgggc cgctccgtcc gtggccacga tcaaccgacg atcagcagcc gccgacagta
     4021 ctcacacctc ctcacgtcct gggtccgctt cagacggtcg atctccatgg ccgagagctg
     4081 ctcgtggcac gcctggcaca cgccgtccac gagccgggcc acggcgaccc ccttcttctg
     4141 agcgcggatc tcctcgtaca gatcgagcaa ctcccggtcg aacgcgggca gcagcccctc
     4201 ccgttcgctc gatcggcgcg agagatcctc ctcgatggag cggagctcct cggccgcgct
     4261 gccgcccagg gcctccagct gctcccgggc tcgcccgagc tccgtctcgg ccgccccggc
     4321 cctgtcctcc agcgcctccc gctgctccat ctggtccagg agccggtcct cggtccggga
     4381 gcgccggtcg cggatgctcg cgatctcgtg ctggagggcc tccagctcct tcgtgttcgc
     4441 gatgctcccg tcgtacagcc gcttctcctc cgccgccgcc ttctgggtca tggagtcgat
     4501 ctcgtgctcc aggcgctgct ggtccctggc gaccgcgtcc agggccaggc ggatctcacc
     4561 tagggcgccc tccgcctgct ccatgctcga gcgcgccacc cggacctcct ccccctcctc
     4621 gagctgccgt cgccgggcct ggaggcgatc gatcgtggtg tcgagggctt gcagctccag
     4681 gatccggtcc atgccctcca tcgggcggaa gggtatcgga aagcgtggcg gccgcacgcc
     4741 cgaccccggc cgcacacccg aggtcacagg atcgtgagtt gacggggtcc gtcgcccgag
     4801 gcctcgagat ccgcgtcccg gcgccaccga ggaggcgcct gcggcgccgg gcgcggaccg
     4861 cccagggcac ggaccagccc gcgtaccctg cgccccagcg cctcgcgatc cgcggacggc
     4921 gcgtacgcct tccggtacag gtcccggtac ctgggaacga ggtccgggta cgtctcctcg
     4981 agccagggca ggaactcctc ccgcacgccg ggacgcaggt gcaggaggat cggagacacg
     5041 tgtgtggccc ccgcgtccag ggcggctcga accacctccc ggagctgacg aggatcgtcg
     5101 ctgatcccgg gcaggatggg cgccatcagg accccgcacg ggatcccggc ctcgttcagc
     5161 gcgcgcacgg cctcgatccg gcgtcggggg tgcggcgtcc ccggctcggt gcgccgccat
     5221 gcatcctcgt ccagggtccc gatggaaaag gcagcggcga cctccgtcac ctcggcggcc
     5281 gccaggagca gctcgaggtc ccgcacgatc aggatgccct tggtgaggat cgagaacggg
     5341 ttccggtact cggtgagcgc ccggaggatc ccaggcatga gtcggtagcg gccctccgcc
     5401 cgctgatagg gatcggtgtt ggtccccatg gctacctgct cgcccttcca gcgcttggcg
     5461 gcgagctcgc ggcgcaggac ctcgggcgcg ttcaccttga ccacgatctt ccgctcgaag
     5521 tccctaccgg cgttcatggc caggtacgtg tgggtgggcc gggcgaagca gttgtggctg
     5581 atcaccccgt ccgcgacgaa gtcgcccgtc ccggtggtga ggtcgaacat cggcaactcc
     5641 tcgccgagcg gctcgacggc ggccacccgg agcggtgcgt cacccttgac caccagccct
     5701 tccaacgacc gctggcgctc gatcgcaggg tcgacggttt ggaggaaggc gaggcgttcg
     5761 cgccacccgc cccgcacccg aacgctcttc aggccgttct cgcgatgcgg gtcctcgatc
     5821 acggcgtcga actccagcgc tcggaggctg gagacgaccc gcccgatgac ctgaggatcc
     5881 gtgttcgaga tgcggatcgc gtcccggccg cagctgcctt cggcgtcgaa gatccccgcc
     5941 aggaaccctt tggaccacga cggggagggc gcgctgggcc gggtgatgat ccatcggatg
     6001 cgctcgatgg ctcccccccg ccgggttcgg atcacgtgga ggtgcctgga tcgttcggac
     6061 ccggggtgga cggggaacgg agtcgtctcg atcccttcca gcgagaggta gcgacgggtg
     6121 cgctccaacc cgtctcggtc ggtgagcgtc agccggaacc cgtgcgccgt acccgggctg
     6181 cgatccgccc tcggatacga gtggtagccc agcgacgcat cgcccttgat cacgccggtc
     6241 aggtacccgc gccggtaggc ctcgtcgtga accggggccg gggcgaacct ccccgggccg
     6301 aggagcgggt cgttcggcgt gaggaacggc cgagggttgc ggccctgttc ggcgccggtg
     6361 acgtgcttcc acccccgacc ggacaggaag cggtgatccc cactcgcgat cagctcggtc
     6421 ccatcctgga gcgtcactcg gtagggggac ttcgcgctct cccactgggc gaggacctcg
     6481 gtggtcacgt accggcgatg gcagcctcgc cgctccgtac cgtacacgc
//
LOCUS       DASQQQ010000229         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_184141, whole genome shotgun sequence.
ACCESSION   DASQQQ010000229 DASQQQ010000000
VERSION     DASQQQ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184141"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..613
                     /locus_tag="VE646_08885"
     CDS             122..613
                     /locus_tag="VE646_08885"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HZD80139.1"
                     /translation="MGDRLATAVRRGERLVLATGHPVGLAHLYIEVGRQLRSRGVEIL
                     QPADGATWRDGDRHHPWQIRYLEGVALLTDKASARHTHSPDPMSRMLDEVRPDLVFAD
                     HGFAGAAIEHGVETVSIADVNDPALLVARAQGRTETVIVMDDNVSPEDYWPCFQAIAS
                     RLP"
     gene            829..1719
                     /locus_tag="VE646_08890"
     CDS             829..1719
                     /locus_tag="VE646_08890"
                     /inference="COORDINATES: protein motif:HMM:NF027889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide 5'-hydroxyl-kinase"
                     /protein_id="HZD80140.1"
                     /translation="MNEHEAVVDQAVRTKDTVLLVGGLDTGKSTLARTLLAAALEAGR
                     SAAFLDADVGQKSVGPPATVTLKLLRSPGDLEPEVLPDADALSFVGSTSPEGHLLPLV
                     AGVANLYRRAKDAGTGFVVVDSCGMVSGIYAQMLHFHEVEALGPDLVVGLQRGEELLP
                     LLGVIQRFFATEVIPLGVHPGVVPTTVDQRAENRERAMARYFGGELHRFRVKPTVFMP
                     ALPPLFDLANLHRLLVGLSDGAGSTTGLGFLEHTSDEGVLRLISPVAEGPKALRLGSV
                     RLEDSFRAKRVDLRNLFGSG"
     gene            1739..1840
                     /locus_tag="VE646_08895"
     CDS             1739..1840
                     /locus_tag="VE646_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007465623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HZD80141.1"
                     /translation="MPSLIKKRRKKMRKKKHKKLLKKTRWQRRQQGR"
     gene            1856..2611
                     /locus_tag="VE646_08900"
     CDS             1856..2611
                     /locus_tag="VE646_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZD80142.1"
                     /translation="MRTTRAGSIGVRVLGVTAVLVLLGGCSDREPALVDLSGSEPVSF
                     SATDGVRLEGRLFGEGSAAVVLSHMRPADQRSWFGFATRLADDGYLVLTYDFRGYCPG
                     DEGGCSEGETDIDALWRDVLGAVTFVRERGADRVALVGASMGGTASLVAASQLGADVD
                     VVITLSAPASTEGLVVDQAVLAEVGAATLFLAGVGDAPYAEDAQTLYALAQQPKRVEI
                     LPSDDHGTDLLTGGQGEVVRRTIENYLAQHGGP"
     gene            complement(2615..2767)
                     /locus_tag="VE646_08905"
     CDS             complement(2615..2767)
                     /locus_tag="VE646_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80143.1"
                     /translation="MRRTTLIAIIVLFVVITISAVYQLMLATRDRERYPGPAPGTPLP
                     TFAPAP"
BASE COUNT          397 a          974 c         1053 g          399 t
ORIGIN      
        1 ggcgccccac ctcgatgtac aggtgcgcca gccccaccgg gtgcccggtc gccaggacga
       61 gccgctcgcc cagccggacc gccgtcgcga gccggtcccc cacctggtgg catgcctcca
      121 ggtgggagac cggctcgcga cggcggtccg gcggggcgag cggctggtcc tggcgaccgg
      181 gcaccccgtc gggctggcgc acctgtacat cgaggtgggg cgccagctcc gttcgcgcgg
      241 cgtcgagatc ctccagccgg ccgacggcgc gacgtggcgg gacggcgatc gccaccaccc
      301 gtggcagatc cggtacctgg aaggcgtggc cctgctgacc gacaaggcga gcgcccgcca
      361 cacccacagc ccggacccca tgagccggat gctcgacgag gtgcgtcccg acctggtgtt
      421 cgcggatcac gggttcgctg gagccgcgat cgaacacggg gtggagaccg tctcgatcgc
      481 ggacgtgaac gatcccgccc tgctcgtcgc ccgggcgcag gggcggaccg agaccgtcat
      541 cgtgatggac gacaacgtgt cccccgagga ctactggccc tgtttccagg cgatcgcctc
      601 ccgcctgccg tgatcggcgc ggggtcgggg ccgacggtag gatgaccccg cagtgaccga
      661 tcaccacgac actcgaggca cggttcgacc gcgtgaacgg gccctcgtgg cccccgtgaa
      721 ggcatcccgg gtaccggcga cgacgagacg agtggaggat ccctcgggct agcgaccgga
      781 gcgcacgcgg gcggaacggg ccttcggggc gagaacccgg aggcggcagt gaacgagcac
      841 gaggcggtcg tcgaccaggc ggttcggacc aaggacaccg tcctgctcgt cggcgggctc
      901 gacaccggca agagcaccct cgcccggacc ctgctcgccg ccgcgctgga ggccgggcgg
      961 tccgcggcgt tcctggacgc cgacgtcggc cagaagtcgg tgggccctcc agcgacggtg
     1021 acgttgaagc tcctgcggtc ccccggcgac ctggagcccg aggtcctgcc cgacgcggac
     1081 gcgctgtcct tcgtggggtc gacctcgccg gagggtcatc tgctcccgct cgtggccggg
     1141 gtcgccaacc tgtaccgcag ggccaaggac gcggggaccg gattcgtggt ggtggattcc
     1201 tgtgggatgg tgtcggggat ctacgcgcag atgctccact tccacgaggt ggaggcgctc
     1261 gggcccgacc tcgtggtcgg gctccagcgg ggcgaggagc tcctgcccct cctgggcgtg
     1321 atccagcggt tcttcgccac cgaggtgatc cccctcgggg tccatccggg cgtggttccg
     1381 accaccgtgg accagcgggc ggagaatcga gagcgtgcga tggcccggta cttcggcggg
     1441 gagctccacc gcttccgggt gaaaccgacg gtcttcatgc ccgcccttcc gccgctgttc
     1501 gacctggcga acctgcaccg gttgctcgtc gggctctccg acggcgccgg ctccaccacg
     1561 ggcctcggct tcctcgagca cacgtcggac gagggggtgc tccggctgat ctcccccgtg
     1621 gcagaggggc cgaaggcact tagacttggg tccgttcgcc tggaggacag cttccgcgcc
     1681 aagcgggtcg acctccggaa cctgttcggc tcgggatgac cagacagaag ggacacgcgt
     1741 gccgtcgctc atcaagaagc gccgcaagaa gatgcggaag aagaagcaca agaagctgct
     1801 gaagaagacc cgctggcagc ggcgccagca gggccgctag gggcacaccc gggcgatgcg
     1861 cacgacgagg gccggctcca tcggggtccg ggtcctcggg gtgacggccg tcctggtgct
     1921 gcttggcggc tgctccgatc gggagccggc cctcgtcgat ctctccggct ccgaaccggt
     1981 ctcgttctcg gcgaccgacg gcgtccgcct ggagggtcgt ctcttcggcg agggctcggc
     2041 ggccgtggtg ctgtcgcaca tgcgaccggc ggaccagcgc tcgtggttcg gcttcgcgac
     2101 gcggctcgcc gacgacgggt acctggtcct cacctacgac ttccgcggct attgccccgg
     2161 agacgaaggc gggtgctcgg agggcgagac cgacatcgac gcgctgtggc gggacgtgct
     2221 cggcgcggtg acgttcgtgc gcgagcgcgg agccgatcgg gtggcgctcg tgggtgccag
     2281 catgggcggc acggcatccc tggtggccgc gtcgcagctc ggtgcggacg tggacgtcgt
     2341 gatcacgctc tcggcgccgg catccaccga gggcctcgtg gtggaccagg cggtgctcgc
     2401 ggaggtcggg gcggcgacgc tgttcctggc gggcgtgggc gatgctccgt acgccgagga
     2461 cgcccagacg ctctacgcgc tcgcgcagca gcccaagcgg gtggagatcc tcccctccga
     2521 cgaccatggc accgacctgc tcaccggggg tcagggcgag gtggttcggc gcacgatcga
     2581 gaactacctg gcgcagcacg gcgggccctg agccctacgg cgccggcgcg aacgttggca
     2641 atggcgtgcc cggcgccggg cccggatagc gctcccgatc gcgggtggcc agcatcagct
     2701 ggtagacggc ggagatcgtg atcaccacga acagcacgat gatcgcgatc agggtggtcc
     2761 ggcgcacggt ccgaggctag caggggccgg ggcgcgtggc gccccggccc ctgcgccggg
     2821 gtc
//
LOCUS       DASQQQ010000230         2943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_93953, whole genome shotgun sequence.
ACCESSION   DASQQQ010000230 DASQQQ010000000
VERSION     DASQQQ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93953"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VE646_08910"
     CDS             complement(<1..590)
                     /locus_tag="VE646_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZD80144.1"
                     /translation="MDAMPWPFGPYPPGGLEELGPHVIAYYAGGFPASNSAIVRGTEG
                     ALVFDPNCFRFAEELRAAADEIGIRPGVTVLSHAHDDHTMGTERFSPPARVLSRSKAR
                     ERLQRNLSEGRRPGAQYERGYPGAAREAEGVRIVVPGETVERPTVLDLGGGVTVRLQP
                     EELAHTDGDLWALVEPDGVALCGDLWYARCEPYVGSG"
     gene            704..1930
                     /locus_tag="VE646_08915"
     CDS             704..1930
                     /locus_tag="VE646_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZD80145.1"
                     /translation="MAEFNVDPYVNLYARRTSGMSASEVRALFAVASRPEVVSLAGGM
                     PYVQALSSEHVLEIAESILAERPHEALQYGGGQGAPALRARLAMLMSEEGTTVDPEDL
                     LVTTGAQQALDLLGKILIDPGDQIAVEAPAYVGALSAFSAYEPDFVTVELDDHGMVVE
                     QLEEALLHGARPKFVYTVPNFHNPAGVTMSLRRREELVALCREAHVPIVEDNPYGLLR
                     FEGASLPTLRSMDPENVIYLGTVSKVFSPGVRVGWAVAQPGLLQRLILAKEAGDLCGS
                     NLTMLLTERYLAGDLWRSNLERLTGLYRSRRDAMLAALSEHFPSQAAWTTPAGGFYVW
                     ATVPDHLDTQALLAAAVERRVAYVPGTAFYPDGRGRDAMRLSFCYPTEADIGEGIRRL
                     GELLREEEELFRSLSR"
     gene            1927..2901
                     /locus_tag="VE646_08920"
     CDS             1927..2901
                     /locus_tag="VE646_08920"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HZD80146.1"
                     /translation="MSAPPPSSIAVLAGGRSPEREVSLRSGHRVETALRSKGYEAFVL
                     DPADGRLVETLSSARISGCYVALHGRDGEDGTVQRVLELLGVPYTGTGPRACQITFDK
                     VLMKESLLEAGVRTPAWATIQAAAVRDLSAGPVLSRVSERVELPAVVKPSRAGSAMGI
                     SFVRDERELPHAVMSALAFSDAVIVEHQVRGTEVAAAMIGAADEPLPLVEVVPRSGVF
                     DYAARYTPGATEYHAPARLSPEFTAAVHREASAAFRALGLRDLGRADVMLDERGGPWV
                     IDVNISPGMTDTSLLPMAARAAGLDLADLCERILDLGLQRAGEPARPD"
BASE COUNT          408 a         1033 c         1055 g          447 t
ORIGIN      
        1 ccgctgccca cgtagggctc acagcgcgcg taccacaggt ccccgcagag ggccacgccg
       61 tcgggctcca ccagcgccca cagatcgccg tcggtgtggg cgagctcctc cggctgcagg
      121 cggacggtga ccccgccccc caggtccagc acggtcggcc tctccacggt ctccccgggg
      181 acgacgatcc ggaccccctc cgcttcccgt gccgctccgg ggtaccctcg ctcgtactgc
      241 gcgcccggac gcctgccctc ggaaaggttg cgctgcaaac gctcccgcgc ctttgatcgt
      301 gagagcacac gcgccggagg gctgaagcgc tcggtgccca tcgtgtggtc gtcgtgagcg
      361 tgcgacagga ccgtcacccc cggccgaata ccgatctcgt ccgctgcggc ccgcagctcc
      421 tccgcgaagc ggaagcagtt ggggtcgaag acgagcgccc cctccgtgcc ccgcacgatg
      481 gccgagttcg acgccgggaa ccctcccgcg tagtaggcga tcacgtgcgg tccgagctcc
      541 tccagcccac ccggcggata gggaccgaac ggccagggca tggcgtccat cgtgggggca
      601 ggctacacgg agcggtggcc gggggagcct ggtggttcgg cgtcccgacg gcgatgctcg
      661 acctcccccg gtggcggccg acgcgttgct agcatgggcc accatggccg agttcaacgt
      721 cgacccctac gtgaacctgt acgcgcgcag gacctcgggc atgtcggcct ccgaggtccg
      781 agcgctgttc gcggtggcct cccgtcccga ggtggtgtcc ctggccggcg ggatgcccta
      841 cgtgcaagcg ctatcgagcg agcacgtgct ggagatcgcc gagtcgatcc tggcggagcg
      901 tccccacgag gccttgcagt acggcggcgg ccagggagcg cccgccctgc gggcccgact
      961 ggccatgctg atgtccgagg aggggaccac ggtcgacccg gaggacctgc tggtcaccac
     1021 cggggcacag caagccctgg acctgctggg caagatcctg atcgatccgg gggatcagat
     1081 cgcggtcgag gccccggcct acgtcggggc gctgtccgcc ttcagcgcgt acgagcccga
     1141 cttcgtgacc gtggagttgg acgaccatgg catggtggtg gaacagctcg aggaggcgtt
     1201 gctccacggg gccaggccga agttcgtata tacggtgccg aacttccaca accccgcggg
     1261 cgtcaccatg tcgctcaggc gtcgggagga gctcgtggcc ctgtgccggg aagcccatgt
     1321 cccgatcgtc gaggacaacc cgtacgggct gctccgcttc gagggggcct cgctgcccac
     1381 cttgcgctcc atggaccccg agaacgtgat ctacctgggc accgtgtcca aggtcttctc
     1441 gccgggtgtc cgcgtgggct gggccgtcgc gcagcccggc ctgctccagc gcctgatcct
     1501 ggccaaggaa gcgggcgacc tgtgcggcag caacctcacc atgctcctga cggagcgata
     1561 cttggccggc gacctgtggc gttccaacct ggagcgactg accgggctgt accggtcgcg
     1621 ccgtgatgcc atgctcgcgg ccctctccga acacttcccg tcacaggcgg cgtggaccac
     1681 gccggccggg ggcttctacg tatgggcgac cgtgcccgac catctcgata cccaagcgct
     1741 gctggcggcg gcggtcgaac gccgggtggc gtacgtgcct gggacggcgt tctatccgga
     1801 cggccgcggc cgggacgcga tgcggctctc gttctgctat ccgacggaag ccgatatcgg
     1861 ggaaggcatc cgccgcctgg gcgaactcct ccgtgaggag gaggagctct tccgctcgct
     1921 gtcgcgatga gcgctccccc accgagctcg atcgccgtcc tcgcgggtgg ccgctcgccc
     1981 gagcgggagg tgtcgctgcg gtccggacac cgcgtcgaga cggccctgcg ctccaagggc
     2041 tacgaggcgt tcgtgctcga ccccgccgac ggacggttgg tcgaaacgct ttccagcgca
     2101 aggatttccg gatgttacgt ggcgttgcac gggagggacg gtgaagacgg gacggtccag
     2161 cgagtcctcg agctgctcgg tgtgccgtac acgggcacgg gaccgcgagc ctgtcagatc
     2221 accttcgaca aggtgctgat gaaagagtcg ttgctggagg ccggggtgcg gaccccggcg
     2281 tgggcgacga tccaggccgc ggccgtccgc gatctctccg ccggccccgt gctgtcccgc
     2341 gtgagcgagc gggtggagct gcccgccgtg gtgaagccct cccgggccgg gtcggccatg
     2401 gggatctcct tcgtgcgcga tgagcgggag ctgccccacg ctgtgatgag cgccctggcc
     2461 ttctccgacg ccgtgatcgt ggagcaccag gtccggggaa ccgaggtcgc ggccgccatg
     2521 atcggcgcgg cggacgagcc gctgccgctg gtcgaggtgg tgccccggtc gggggtcttc
     2581 gactatgccg ccaggtacac gcctggggcc accgagtacc acgccccggc gcgcctgtcc
     2641 cccgagttca ccgccgccgt ccaccgcgag gcctcggccg ccttccgagc actcgggctc
     2701 cgcgatctcg gtcgggcgga cgtgatgctg gacgagcgcg ggggcccgtg ggtgatcgac
     2761 gtcaacatct cgcccggcat gaccgacacc agcctgctcc cgatggccgc tcgggccgcg
     2821 gggttggacc tggcggatct gtgcgaacgg atcctcgatc tcggcctcca acgcgcagga
     2881 gagccggcgc ggcccgacta gtccggccca gactaggccg gggggggggg gggggggggg
     2941 ggg
//
LOCUS       DASQQQ010000231         3458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_315043, whole genome shotgun sequence.
ACCESSION   DASQQQ010000231 DASQQQ010000000
VERSION     DASQQQ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315043"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VE646_08925"
     CDS             <1..842
                     /locus_tag="VE646_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HZD80147.1"
                     /translation="VALFNILEERDVQIRGYRIRLPLDLLLVATANPDDYTHRGRIVS
                     PLKDRFGTQVRTHYPETLDEEILIMDQEARPSVGDVPVRVPRYLKEVLAALTAELRRS
                     PHVNHRSGVSVRYSIGNLETLSAASVRRAARVNEPEAVPRVCDLPAVVDASLGRVEFD
                     TIEEGREEEILGRALRTAELEVFRRRLSGFDLQPFLSRFTGGFVAETSDLMPATDVLN
                     QFGDLPGLSRLLERLGVEEESPGVAASAVEFALEGLHLSRRLNKDRTDRPGSYRYGSG
                     ESS"
     gene            855..1226
                     /locus_tag="VE646_08930"
     CDS             855..1226
                     /locus_tag="VE646_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80148.1"
                     /translation="MPDVHEVVGFSVVALFTLGWLWGIALWALRRGAGSWFWRWLALA
                     QIVAGLQAVLGIALLVLGYRPDTWLHLVYGFGPLIVLGFAHVAARTEMYAERPWTPFA
                     WGSFICFGLTLRALMTGLGIG"
     gene            1326..1802
                     /locus_tag="VE646_08935"
     CDS             1326..1802
                     /locus_tag="VE646_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80149.1"
                     /translation="MRRELVGRLDPWSYWVVDLGHELPADLAVVGTTGAFGILVDGRE
                     GYLEADGGKLSVDRRPISGFRDAKTAAKRLRSRLGQAAVYTEVVPMICLSRAVAGASR
                     TIRGVRVVRLQDLAGEIAGREKSLSANRARRGAEALGSVVATGQGARPEVEDPEHH"
     gene            1864..>3458
                     /locus_tag="VE646_08940"
     CDS             1864..>3458
                     /locus_tag="VE646_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80150.1"
                     /translation="MRTRYSRWDGSQDPFGPDIPAAELLEEMSEDMLSGAGAQGAMSR
                     LLHRGMRGRFGGLDALRARLREARARERARLDLEGPLEDLRERLDEIIDRERSTLSFR
                     AEEDARMREAFLDQLPSDVPGQIGELSDYRFTDPRAQEQFDALMEHLKEQVLGAYFRN
                     MAQGLRGSSPEALQRLKDMLADLNELIERRERGDLSQEDFDRFMQRHGDFFPDRPRTL
                     DELLESMARRMAAMSRLLASMSPEQRAQLQALAEQMMQDMDLAFEVDRLGSNLSGLFP
                     GLSWGEPSPGGGEETAPLSATVDAMERLHDYEDLDRAMRGDYAGASLDDIDEDAVRRT
                     LGEDAVRDVRRLKQIERALEQAGLVTRREGRLEVTAKGARKLGERALVRVFEQLRRDR
                     EGTHAAREAGGLAEPTGATRPWHFGDSGQIAVQRTVFNAVLRTGRGAEVRLAPDDFEL
                     VDAEQRTESATALLLDLSFSMPLRGHFVHAKRMALALHALIEGRYPHDTLYLIGFSDY
                     ARPMQPQDLTAAGWERVYGTNMQ"
BASE COUNT          487 a         1122 c         1292 g          557 t
ORIGIN      
        1 aggtcgccct gttcaacatc ctggaggagc gcgacgtgca gatccgcggg taccggatcc
       61 gcctgccgct ggacctgctc ctggtggcca ccgccaaccc cgacgactac acgcaccggg
      121 ggcggatcgt ctcgccgttg aaggaccggt tcggcacgca ggtgcggacc cactatccgg
      181 agacgctgga cgaagagatc ctcatcatgg accaggaggc caggccctct gtcggtgatg
      241 tgcccgtgcg cgttccccgg tacctgaagg aggtgctggc cgcgctcacc gcggagctcc
      301 ggcgctcgcc acacgtcaac catcgatccg gcgtttcggt ccggtactcc atcggcaacc
      361 tggagacgct gtcggccgcg tcggtgcggc gcgccgcacg ggtgaacgag cccgaggcgg
      421 tccctcgggt ctgtgatctt cccgccgtgg tggacgcctc gctgggacgg gtggagttcg
      481 acacgatcga ggagggacgt gaggaggaga tcctgggccg ggcccttcgg acggcggaac
      541 tggaggtgtt ccgacgtcgg ctctccggat tcgatctcca gccgttcctg agccggttca
      601 ccggtggctt cgtggccgag acgtcggatc tgatgccggc cacggacgtc ttgaaccagt
      661 tcggagacct ccccggcctg tccaggctgc tggagcggct cggcgtggag gaggagagcc
      721 cgggggtggc cgcatcggcg gtggaattcg cgctcgaggg cctgcacctg tcccgtcggc
      781 tgaacaagga tcggaccgac cggccgggtt cgtaccggta cggatcgggt gagagctcgt
      841 aacatcccct ggccatgccg gatgtccacg aggtcgtcgg gttcagcgtc gtcgcgctgt
      901 tcacgctggg ctggctctgg ggcatcgctc tctgggccct ccgtcggggc gcggggtcgt
      961 ggttctggcg atggctggcc ctggcccaga tcgtggccgg gctgcaggcg gtgttgggga
     1021 tcgccctgct cgtcctcggc taccggccgg acacgtggct acacctcgtg tacgggttcg
     1081 gacccctgat cgttctgggc ttcgcccacg tcgccgcccg cacggagatg tacgcggagc
     1141 gcccctggac gcccttcgcc tggggatcgt tcatctgctt cgggctgacg ctccgcgcct
     1201 tgatgacggg tctcggcatc ggatagcgtc gttctcgccc catcctggac agggaccagc
     1261 ccatgtcgct ctctcgatcg ctacgggatc cccgccccga gcccgacccg cgcgaggtgg
     1321 gccgggtgcg cagggagctg gtgggtcgcc tggacccttg gagctattgg gtggtcgatc
     1381 tggggcatga gctgcccgcc gacctcgccg tggtgggcac gaccggggcc ttcgggatcc
     1441 tggtcgacgg ccgtgaggga tacctggagg ccgatggcgg caagctgtcg gtggatcgtc
     1501 gtccgatctc ggggttccgc gacgccaaga cggccgccaa gcggctgcgg tcacggctgg
     1561 gccaggccgc cgtgtacacg gaggtggtcc ccatgatctg cctttcccgg gccgtggccg
     1621 gtgcctcccg tacgatcagg ggggtacgcg tcgttcggct gcaggacctg gcgggggaga
     1681 tcgcggggcg cgagaagtca ctctcggcca accgagcccg acgtggcgcc gaggctttgg
     1741 gcagcgtggt ggccaccggt cagggggccc ggcccgaagt cgaggatccc gagcatcact
     1801 agccctcgcg cccgcacgct ccagcggcgc ccgttcggat gccgccggac gtaggctgga
     1861 tggatgcgga cccgatactc gcgctgggat ggcagtcagg accccttcgg ccccgacatc
     1921 cccgccgcgg aactgctgga ggagatgtcc gaggacatgc tctcgggcgc cggagcccag
     1981 ggagcgatgt ctcgcctgct gcaccggggg atgcgggggc gattcggcgg gctcgatgcg
     2041 cttcgtgcca ggctgcgcga ggcgcgtgcc cgggagcgcg cgcggctcga cctggagggc
     2101 ccgctcgagg atctgcggga gcggctggac gagatcatcg atcgagagcg ttccaccctg
     2161 tccttccggg cagaggagga tgctcggatg cgcgaggcgt tcctggacca gctgccttcc
     2221 gacgtcccgg gccagatcgg cgagctgagc gactaccggt tcaccgatcc ccgggcccag
     2281 gagcagttcg atgcgctcat ggagcacctg aaggagcagg tgctcggggc ctacttccgg
     2341 aacatggccc aggggctccg gggttcctca cccgaggctc tccagcggtt gaaggacatg
     2401 ctcgccgacc tgaacgagct gatcgagcgg cgggagcggg gcgatctctc gcaggaggat
     2461 ttcgatcggt tcatgcagcg gcacggggac ttcttcccgg accggccccg gaccctcgat
     2521 gagctgctcg agagcatggc tcggcggatg gcggccatgt cccgtctgct ggcatccatg
     2581 tctcccgagc agcgggcgca gctgcaggca ctggccgagc agatgatgca ggacatggac
     2641 ctggccttcg aggtggatcg gttgggctcg aacctgtccg ggttgttccc ggggctgtcc
     2701 tggggcgagc cgtcgccggg cggtggcgag gaaaccgcgc ccctgtccgc cacggtcgat
     2761 gcgatggagc gcctccacga ctacgaagac ctggaccggg cgatgcgagg agactacgcg
     2821 ggtgcgtcgc tggatgacat cgacgaggac gcggtccgcc ggacgctggg agaggacgcc
     2881 gtgcgtgatg tccgacgcct gaagcagatc gagcgcgcgc tcgagcaggc cgggttggtg
     2941 acgcgccgcg agggccggct ggaggtcacc gcgaagggcg cccgcaagct gggggaacgg
     3001 gcgctggtcc gggtgttcga gcagctgcgg cgcgaccggg agggtacgca cgcagctcgg
     3061 gaggcggggg gcctggcgga gcccacgggc gccacccgtc cctggcactt cggggacagc
     3121 gggcagatcg ccgtccagcg caccgtgttc aacgcggtgc tgaggaccgg ccggggggcg
     3181 gaggtccggc tggcaccgga cgacttcgag ctggtggacg ccgagcagcg gacggagtcg
     3241 gccaccgcgc tgctgctcga tctgtcgttc tccatgccgc tccggggcca cttcgtgcat
     3301 gcgaagcgga tggccctggc cctgcatgcg ctgatcgagg ggcggtaccc gcacgacacg
     3361 ctgtacctga tcggtttcag tgactacgcg cgcccgatgc agccccaaga cctgaccgca
     3421 gcgggatggg agcgcgtcta cggcacgaac atgcagca
//
LOCUS       DASQQQ010000232         3915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_128187, whole genome shotgun sequence.
ACCESSION   DASQQQ010000232 DASQQQ010000000
VERSION     DASQQQ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128187"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..824)
                     /locus_tag="VE646_08945"
     CDS             complement(54..824)
                     /locus_tag="VE646_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020020196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HZD80151.1"
                     /translation="MKLKPPSPGEVLDRVRETEIWKSIFRPGSIFRKGYRDTSRDRAL
                     ATMNNVLYHLHPVKMKRHGLKLTYTFCLGGLSFFLFILLTITGIFLMFFYRPAADIGS
                     ATAYADMQSIRTSVFFGDLVRNLHRWGAHLMVFSVTLHMARVFYTGAYKPPREFNWVV
                     GVVLLFLTLGLSFTGYLLPWDQLAIWAVTVGTSLAGYSPLIAKQANFLLLGGVVVGPE
                     TLLRWYVLHVLALPFVLVIFLAVHFWRIRKDGGISGPL"
     gene            complement(874..1269)
                     /locus_tag="VE646_08950"
     CDS             complement(874..1269)
                     /locus_tag="VE646_08950"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZD80152.1"
                     /translation="MPFTKTEVDIHTDYVVEAVDPSWLSQGVKSPKHLMLDPDNCILC
                     RACEDVCPWNCIYMMSTGIVQGTEDQAVSAEVGNATAVFVVDDNACTRCSVCVERCPT
                     DTLYYARLPEDSGGRRQMAAVPMSQKADA"
     gene            complement(1292..1645)
                     /locus_tag="VE646_08955"
     CDS             complement(1292..1645)
                     /locus_tag="VE646_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80153.1"
                     /translation="MKVPNKLVELRDQRIEPFLKSFEWTWTTAVVFSLALAFYMILFV
                     AVVPSFWMYFAEQKLGWGGPSGGGDWLLELRDAIAMGITTVFFAIPFVAAAVMQNWRR
                     RLRGRSAERPGGGYR"
     gene            1843..2586
                     /locus_tag="VE646_08960"
     CDS             1843..2586
                     /locus_tag="VE646_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HZD80154.1"
                     /translation="MDPVIADLIQRGLASWWAPGLAFAAGLVSFASPCVFPLVPGYLS
                     FVSGGQAREEQPVIPILLFIAGFAAVFTALGAFTGSVQRFLRSPTAVRVEGLIIVLFG
                     VLMVLYAFRLGWPGLYAERRPLLGRVHPGRGWAFPLGIAFGAGWTPCIGPVLAGVLGI
                     AAAQGGSARGALLLLMYSAGLGLPFLLLGLGIRRLVGALELLKRNYHWIAGVSGAVMV
                     AIGVLIVTDLWVRWLAPLLRAIGRFTPPI"
     gene            2633..>3915
                     /locus_tag="VE646_08965"
     CDS             2633..>3915
                     /locus_tag="VE646_08965"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HZD80155.1"
                     /translation="MSVSNAQAPLTSLRRSLALVWRTLRSMRTALVLLLLLALAAVAG
                     SLVPQTGTSDARIAAMFVDHPLRARLYEQLGLFDVYGSWWFTLIYTLLLVSLVACLIP
                     RTRALIRNLRARPQPARELDTMRHYAERSVAGGPHEVIRTSRGVLRRKLFRVSGSNGT
                     GPPSLAADKGLAREAGSLLFHWAFLLILVGIVWGKGTGFSGKAVIVEGQTWTEAHANY
                     DGQLQEGRFFGEDHTGVQVHVDSFTARYRIPSGIPEDFVTHAELSGPDGGPARSVEIR
                     PNHPAEIDGIKLYQFGYGWAPVIDVSSRGEPLASGPVVCQQGAAPKGVSQLQLPWSCV
                     VKLPTLRPQVGIRFQLWPDSRALYALLDRGQPMPMIGQFAPVMTYRAYRGDLRAEQVQ
                     GIAELDTSAMREFQSGVIGAGKTVTLGAGLTVSFP"
BASE COUNT          574 a         1359 c         1346 g          636 t
ORIGIN      
        1 ctgatcgagc ggggtgtcct ctcgcgccat gtcgtacctc agcccttcac ggtctacagc
       61 ggtcccgaga tcccgccgtc cttgcggatc cgccagaagt ggaccgcgag gaagatcacc
      121 aggacgaacg ggagggccag cacgtggagc acgtaccagc gcaacaacgt ctccgggccc
      181 accaccaccc cgcccagcag gaggaagttg gcctgcttgg cgatgagcgg ggagtagccg
      241 gccaggctcg tccccacggt gaccgcccag atggccagct ggtcccaggg cagcaggtag
      301 ccggtgaacg agagccccag ggtgaggaac aggagcacca cgcccaccac ccagttgaac
      361 tcgcgcggcg gcttgtacgc ccccgtgtag aacacccgcg ccatgtgcag ggtcacgctg
      421 aacaccatca gatgggcgcc ccaccggtgc aggttccgga ccaggtcccc gaagaagaca
      481 gaggtccgga tgctctgcat gtccgcgtac gcggtggccg agccgatgtc cgcggcgggc
      541 cggtagaaga acatcaggaa gatgccggtg atcgtgagca ggatgaagag gaagaacgag
      601 agccccccca ggcagaacgt gtaggtgagc ttcagcccgt gacgcttcat cttcacgggg
      661 tgcaggtggt acaggacgtt gttcatggtg gccagggcgc ggtcacggga cgtgtcgcgg
      721 tagcccttcc ggaagatcga cccgggacgg aagatcgact tccagatctc ggtctcccgg
      781 acccggtcca agacctcgcc gggggacggc ggcttgagct tcaccctggc gttccccctg
      841 tccttccgtt ctccgcgggc accggcacga cgatcaggcg tccgccttct gcgacatcgg
      901 caccgcggcc atctggcgcc tgccgccgga gtcctccggc agccgcgcat agtacagcgt
      961 gtccgtgggg cagcgctcca cgcacaccga gcagcgggtg cacgcgttgt cgtccaccac
     1021 gaacacggcc gtggcgttgc cgacctccgc gctcaccgcc tggtcctcgg tcccctgcac
     1081 gatgccggtg gacatcatgt agatgcagtt ccacggacag acgtcctcgc aggcccggca
     1141 caggatgcag ttgtccgggt cgagcatgag gtgcttgggg gacttgaccc cctgcgacaa
     1201 ccagctgggg tcgaccgcct ccaccacgta gtcggtgtgg atgtcgacct cggtcttcgt
     1261 gaagggcatc gggagacccc ctagctcgtc ctcatcggta cccaccgccg ggtcgctccg
     1321 ccgaacgtcc ccgcagccgg cggcgccagt tctgcatgac ggcggcggcc acgaacggga
     1381 tcgcgaagaa gaccgtggtg atccccatcg cgatggcatc gcgcagttcg agcagccagt
     1441 ccccgccgcc cgatggtcct ccccagccga gcttctgctc cgcgaagtac atccagaagg
     1501 agggcaccac cgccacgaac aggatcatgt agaaggccag tgcgagggag aacacgacgg
     1561 cggtggtcca cgtccactcg aagctcttga ggaacggctc gatgcgctga tccctcagct
     1621 ccacgagctt gttcggaacc ttcatcggcg acccatccta gcgttcggcc ggcagatgat
     1681 agaccaccgg ccttccagcg acccccacac gcctatcgtt cgtccatcgc accgcgtctt
     1741 gtgaagagct tcacaagcgg cagaccccca ctatggaacg cgagcgccca ggctgcaacc
     1801 cccccgccgc gagctcaacg ttcctccgta cactcgtggg acatggaccc ggtgatcgcc
     1861 gacctgatcc agcgagggct ggcttcctgg tgggctccgg gcctcgcctt cgcggccggc
     1921 ctggtgtcct tcgcctcccc gtgcgtgttc ccgctcgtcc ccggctacct gtcgttcgtc
     1981 tcgggcggtc aggctcgcga ggagcagccg gtgatcccca tcctgctgtt catcgccggg
     2041 ttcgccgcgg tcttcaccgc gctcggcgcg ttcaccgggt ccgtgcagcg gttcctgcgc
     2101 tcgcccaccg cggtccgggt ggaggggctg atcatcgtcc tgttcggcgt gctcatggtc
     2161 ctgtacgcgt tccggctggg gtggccgggc ctgtacgcgg agcgccggcc cctgctgggc
     2221 cgggtccacc ccggacgtgg atgggcgttc ccgctgggca tcgcgttcgg cgcgggctgg
     2281 acgccgtgca tcggtccggt gctggcgggc gtgctgggga tcgccgccgc ccagggtggc
     2341 tcggcccggg gcgcgttgct gttgctgatg tactcggccg ggctgggcct gccgttcctg
     2401 ctcctggggc tcggcatccg tcggctggtg ggggcgctgg agctgttgaa gcggaactac
     2461 cactggatcg ccggcgtctc cggcgcggtg atggtggcca tcggtgtgct gatcgtgacg
     2521 gacctgtggg tgcggtggct cgcgccgctg ctccgagcca tcggccggtt caccccgccg
     2581 atctgacccc ctgccccttc cgggtccgaa cccctacaat cggcgtttcg ggatgtccgt
     2641 gtccaacgcc caagccccgc tcacgagcct ccgccggtcc ctggccctgg tgtggaggac
     2701 cctgcggtcc atgcgcaccg cgctggtgct gctgctcctg ctcgccctcg ccgcggtggc
     2761 gggatcgctc gtcccgcaga cggggacctc cgacgccagg atcgccgcga tgttcgtgga
     2821 ccatccgctg cgcgctcgtc tgtacgagca gctcggcctg ttcgacgtct acggatcgtg
     2881 gtggttcacg ctgatctaca cgctgctgct ggtctccctg gtggcgtgcc tgatccctcg
     2941 gacccgggcg ttgatccgca acctccgggc ccgaccgcag ccggcccgcg agctggacac
     3001 gatgcgccac tacgcggagc ggtcggtcgc gggcggcccc cacgaggtca tccggacttc
     3061 ccgaggcgtg ctgcggcgca agctgttccg ggtgagcggc tcgaacggca cgggcccgcc
     3121 cagcctggcg gcggacaagg gcctggcccg ggaagcgggg agcctgctgt tccactgggc
     3181 gttcctgctg atcctggtgg ggatcgtgtg gggcaagggg accgggttct cgggcaaggc
     3241 cgtgatcgtg gagggccaga cctggaccga ggcccacgcg aactacgacg gtcagctcca
     3301 agaggggcgg ttcttcggcg aggaccacac cggcgtgcag gtccacgtgg acagcttcac
     3361 ggcccggtac cgcatcccgt cggggatccc ggaggacttc gtgacccacg ccgagctctc
     3421 cggaccggac ggcgggccgg cccggagcgt ggagatccgt cccaaccacc cggccgagat
     3481 cgatgggatc aagctgtacc agttcggcta cggatgggcg ccggtgatcg acgtcagcag
     3541 tcggggcgag cccctggcct ccggcccggt ggtgtgccag cagggggccg cgccgaaggg
     3601 ggtcagccag ctgcagctgc cctggagctg cgtggtgaag ctgcccaccc tgcggccgca
     3661 ggtggggatc cggttccagc tgtggccgga cagccgggcg ctctacgcgc tgctcgacag
     3721 gggccagccc atgccgatga tcggccagtt cgccccggtg atgacctaca gggcctaccg
     3781 gggcgacctt cgggcggagc aggtgcaggg catcgcggag ctcgacacga gcgcgatgcg
     3841 cgagttccag agcggcgtca tcggcgcggg caagaccgtg accctggggg cgggcctgac
     3901 cgtgtccttc cccca
//
LOCUS       DASQQQ010000233        10223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_128285, whole genome shotgun sequence.
ACCESSION   DASQQQ010000233 DASQQQ010000000
VERSION     DASQQQ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10223
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128285"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VE646_08970"
     CDS             complement(<1..473)
                     /locus_tag="VE646_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019925341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HZD80156.1"
                     /translation="MLALFNDLVTQEIARYGYLAIFLLMVLESACIPIPSEVTMLFGG
                     ALASAAFVASGGQLNLVAVGLFGALGNLVGSWLAYGIGYAGGRPLVDRWGRYLLLRPH
                     EIDRAHRWFERYGQGAVLLGRLLPVVRTFISLPAGVARMNVWRFSFYTLLGCLPWT"
     gene            688..2982
                     /gene="topA"
                     /locus_tag="VE646_08975"
     CDS             688..2982
                     /gene="topA"
                     /locus_tag="VE646_08975"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HZD80157.1"
                     /translation="MTRTLVVVESPTKAKTLERYLGADFTVRASFGHIRDLPKSKLGV
                     NTEHEFEPEYVVPENAEKAVRELRTALKRADDLVLATDFDREGEAIAYHVAEVLKVDP
                     GTAKRVTFTEITREAILAAFEEPRGIDLRLVEAQQARRILDRLVGYRLSPVLWKKVRP
                     GLSAGRVQSVAVRLIVEREREIRAFVPVEYWSIDARLTPQDAESPFTARVVQVPEGKL
                     AASPDKKGITLGAEADARTHVERLRRAAYRVADVRQKEVKRSPAAPFTTSTLQQEAAR
                     KLGFSARQTMRVAQQLYEGVDLPGEGRVGLITYMRTDSTTIAEAALREIAQVVRSQFG
                     AEYALKEPRRYRTKQRGAQEAHEAVRPTSALRIPDQIQAALDRNQARLYRLIWQRTVA
                     SQMAEARFDQMSVDVEATADEERYLLRATGQTLKFDGFRRVYFEGRDDAVDEDAESML
                     PGLTAQQLLRLLDVLPEQHFTQPPPRYSEASLVKTLEERGIGRPSTYASIISTIQDRG
                     YVRLEEKRFFPEDVGEVVTDLLVEFFPDIVDVNFTSKMEEELDDIAEGKLGWIQVLDE
                     FYGPFERSIEKNVEEIQRFEETLDELCPLCPEEGREPGQLVVKLGKYGKFIGCNKYPE
                     CRYIRNLNGQERKEPEILEETCPECGRPLAKKYGRFGPFVGCTGYPECRYIKKQEQKT
                     GVRCPKCGSGELVVKRARRGKRGSVFYGCNRYPDCDFTVGQKPLPEPCPSCGSLMVQQ
                     KDGSAKCTACGSVVETVEASAAEA"
     gene            2983..5010
                     /gene="tmk"
                     /locus_tag="VE646_08980"
     CDS             2983..5010
                     /gene="tmk"
                     /locus_tag="VE646_08980"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZD80158.1"
                     /translation="MSQLADEVAQLRGTRSTSFKDLLTHRPFARLLAAQTVSSLGDWV
                     GFVAVASLVTRLGGTAGAFAVAGVMMARMLPAILFGPIAGVLVDRFDRKRLMIVADLS
                     RGALYASMPFLGRLWAIYLLSFVIESLSLLWTPSRDASLPKLVPRRQLANANSLGLVT
                     TYGTLPLGGLVFAVLAGVSELFPYVKHDPEAVPLLLDGGTFLFSALMVRGIPIRTPAS
                     RSLGSFDLSRAWGDVVDGFRFLRSHPLASSMTVGIVTAFSAVGAVLAIGPVFVATTLG
                     AKAAGWGVLVTSFGIGMGIGMALVNKLVERVERDLVFVWSMIAAAVTLFVLAAMPNLA
                     LAALLAVFLGVFCGATWVSGYVLLQENVADEYRGRTFGSLTVMSRLGLFLSLTVFPVI
                     AGAIGTHQLFIGGQRIDLSGTRGAMWAAGIIVILAADVTRKGLRRFRLSRPKPLALMP
                     KLKRPPATGTFIVFEGVEGAGKGTQIRLAKEWLESEGVDVLVTREPGGTEVGEKLREL
                     LLDHETGHVEPRTEALLFAASRSQHVATVIRPALAGGKVVICDRYIDSSLAYQGWARG
                     LGEQDVLTLSVWATQGLFPDLVILLHIEPEAGLLRSLEEPDRIEMESGDFHSKVADAY
                     LKIAEEHPERFVVIDADDRPDRVHEQVKQALSRVLREREEEQQQERPEHHE"
     gene            5003..6139
                     /locus_tag="VE646_08985"
     CDS             5003..6139
                     /locus_tag="VE646_08985"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZD80159.1"
                     /translation="MSEAAPLADAAPVLDRVPGQASAMAFLHRATARPHHAYLFAGPE
                     GGGKSLAARAFAAALLCRRRGCGTCRDCRLALDDRHPNEFLVEPEGRDIHVDTIRSEV
                     WHPAHRTAPEPGRKVFIIREADRLNPAAADTLLKVLEEPPADAVLMLLSARPHELSET
                     ILSRCHVVTFQPLAEGFVVETLAAEGAAPERARLAARLSGGNLGRARRLAMDPEGLTF
                     RQVAAQALELAARSAAGGLDAADAVLAAAERYRKGLKDQLEEEIAPFLDDQGRPEDAY
                     RGAIRRLEERHRRRVRRAERDYVDWVLLAMSSLLRDRIVGAVGGSDDLLMNLDHPPAA
                     SEVRASALGVAAVEEARAALAEDLNLNPRLLLEHAFLALGRIAA"
     gene            6226..6603
                     /locus_tag="VE646_08990"
     CDS             6226..6603
                     /locus_tag="VE646_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD80160.1"
                     /translation="MSVRVLIVDDQEPFRMAARMVVEAAEGFEVVGEADTGESSVEMA
                     RELDPDLVLMDVNLPGVNGLEATRRILEDSADRTVVLLLSTYEEEEYAPRAAESGAAA
                     YIPKAVFGPDRLEEAWTQAADAR"
     gene            6791..7198
                     /locus_tag="VE646_08995"
     CDS             6791..7198
                     /locus_tag="VE646_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZD80161.1"
                     /translation="MGRILVGTDTSPSADLAVRAAADLARHTHAELLVLYVRSDGGLR
                     DAADPRKRPDPGRYMADLSARFPELRVRSWVEPGEPAERLCEVAVDEGVDTIVVGNKG
                     AGGSRWRVRESVPTMVVRHAPCSVLIVDTRVAQ"
     gene            complement(7232..7903)
                     /locus_tag="VE646_09000"
     CDS             complement(7232..7903)
                     /locus_tag="VE646_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD80162.1"
                     /translation="MVRVVFAEDNYLVREGTAALLQTTEQVDLVATASTLDELLEAVE
                     AHRPDAVLTDIRMPPTNTTEGIDAARRIRERHPEVGVVVLSQYAEEEYAYELLKDGAA
                     GLGYLLKERVADLGEIVRALDEVARGGSVLDPKVVEALVARKEHLAHSPLAQLTERER
                     EVLGHMAQGKNNAAIAKSLFLTERAVEKHINSLFHKLDLSEEPDVHRRVMAVLAFLRE
                     GERTP"
     gene            7939..9216
                     /locus_tag="VE646_09005"
     CDS             7939..9216
                     /locus_tag="VE646_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZD80163.1"
                     /translation="MSIRVVLGEDNVLVREGVRALLDSYQDVEVVGVAQDAPSLLQTA
                     AEHIPDVVVTDIKMPPNFRLEGIDCAHAIRDKHPDTGVVVLSAHDDEEYAIALLGKGQ
                     SGLAYLLKDRIAQGDELVRAIREVAAGGSAVDPTIAERLSGRHDAEEDDRRILDMMSQ
                     GLGYEQMAERLGTTQEAVDRQVTELFRRLAGGDGGGLVDEFKRLHAAVVEQATSRRTL
                     ASFVPQQLAERLALQPDEPIEPQEVEVSVLFSDIRGFSTIAERLSARDVASLVGRHLS
                     AMAEVVVAHGGTIDKFQGDAVMAVFGAPDPQPDHAERALRCAMAMQARQTELDAQGWG
                     VDGLPTMGVGIGVNTGPVIAGTVGGGGRLEYTVVGDAVNVASRLQSEAEAGEIVASAS
                     TVEAAPGITCESIGPRIVKGREEPVEVFRVRRG"
     gene            9200..>10223
                     /locus_tag="VE646_09010"
     CDS             9200..>10223
                     /locus_tag="VE646_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80164.1"
                     /translation="MSVAAELVPERPARWPWYAFPAFLGAAFVGLVLVAQNDEPLSQQ
                     VPYVVAFTMFWLVGAVIVSRVPRNTIGLFLLYTSAVTATAFLASELTTWLIERGHRGP
                     GVAAIGLLNNLAWLFGFIPVVFILPLLFPDGRLPSKRWKPYVWFVVAFLMLFALTLLF
                     GQRRVFGSGSDPLEIPNPLYIRAIGRLPDLDPLFGTLFPLLFAVSAVSLVLRFHRSKG
                     VERQQIKWVAFGLPVALVGIFVSNYSDPTLVGAVLGAIGFLAFPVSIGIAILRFHLYD
                     LDVVVRKTVVYAALALFATLVYLALVVGLGAWLGRGSSLLTIVAAVVVAAAFQPVRAR
                     LNRFANR"
BASE COUNT         1462 a         3472 c         3674 g         1615 t
ORIGIN      
        1 gtccagggca ggcatcccag cagcgtgtag aagctgaagc gccacacgtt catccgggcc
       61 acgccggcgg gcaggctgat gaaggtccgg accacgggca acaggcgtcc caggagtacg
      121 gcgccctggc cgtagcgttc gaaccagcgg tgcgctcggt cgatctcgtg gggccgcagg
      181 agcaggtacc gtccccaccg gtccaccagg ggacgtcctc ccgcatagcc gatcccgtag
      241 gcgagccagg accccaccag gttgcccagc gccccgaaca gccccacggc caccaggttc
      301 agctgccccc cgctcgcgac gaaggcggcg ctcgccaggg cccctccgaa gagcatcgtg
      361 acctcgctgg ggatggggat gcaggccgat tcgagcacca tcaggaggaa gatcgccagg
      421 tatccgtacc gtgcgatctc ctgggtcacc aagtcgttga agagtgcgag cacgagtcct
      481 cgaggggtgc ggagggaatg gatggagggt atcaccgggt tgggagctgc cggacctcga
      541 ggcgatcggt tggcacggag atatcacacc cggcgcccga ggagccgata atcccccggt
      601 gacgtcgggg gctggtaccc tgcgccggga acctcctcgg ccgccgggcc accacctccc
      661 caagacgcat ggagctggat cgctggtatg acgagaacgc tggtcgtcgt cgagtcgccc
      721 acgaaggcga agacgctcga gcgctacctg ggcgcggact tcaccgtgcg cgcctcgttc
      781 ggccacatcc gcgacctgcc caagagcaag ctcggcgtga acaccgagca cgagttcgag
      841 cccgagtacg tggtcccgga gaacgccgag aaggccgtcc gtgagctacg gaccgccctg
      901 aagcgtgcgg acgacctggt cctggccacc gacttcgatc gcgagggcga ggccatcgcc
      961 tatcacgtgg ccgaggtgct gaaggtcgac cccgggaccg ccaagcgggt gaccttcacc
     1021 gagatcaccc gcgaggccat cctggccgcg ttcgaggagc cccgcggcat cgacctgcgg
     1081 ctggtggagg cgcagcaggc gcgcaggatc ctggaccggc tcgtggggta ccggctctca
     1141 ccggtcctgt ggaagaaggt gcgccccggc ctctccgccg gccgggtcca gtccgtggcc
     1201 gtccgcctga tcgtggagcg ggagcgcgag atccgggcct tcgtcccggt ggagtactgg
     1261 agcatcgacg cacggctcac gccgcaggat gccgagagcc cgttcaccgc ccgggtggtc
     1321 caggtgcccg agggcaagct ggccgcctcc ccggacaaga agggcatcac cctgggtgcg
     1381 gaggccgacg cccggacgca cgtcgagcgc ctgcggcggg ccgcctaccg ggtggccgac
     1441 gtccgccaga aggaggtcaa gcgcagcccc gccgctccct tcaccacctc cacgctccag
     1501 caggaggccg cgcggaagct gggcttctcg gcgcgacaga ccatgcgggt ggcgcagcag
     1561 ctctacgagg gcgtcgacct ccccggcgag ggtcgggtcg ggctcatcac ctacatgcgg
     1621 accgactcca ccaccatcgc cgaggccgcc ctgcgggaga tcgcgcaggt ggtccgctcc
     1681 cagttcggcg ccgagtacgc gctgaaggag ccgcgccggt atcggaccaa gcagcggggc
     1741 gcccaggagg cgcatgaggc cgttcggccg acctcggcgc tccggatccc cgaccagatc
     1801 caggcagctc tggaccgcaa ccaagcccgc ctgtaccggc tgatctggca gcggaccgtc
     1861 gcctcgcaga tggccgaggc gcggttcgac cagatgagcg tggacgtcga ggcgacggcc
     1921 gacgaggagc ggtacctgct ccgggccacg gggcagaccc tgaagttcga cgggttccgg
     1981 cgcgtctact tcgagggtcg ggacgacgcc gtggacgagg acgccgagtc catgctcccg
     2041 ggcctgaccg cccagcagct gctgcgtctg ctggatgtcc tgcccgagca gcacttcacg
     2101 cagcccccac cccggtactc ggaggcctcg ctggtcaaga ccctggagga gcggggcatc
     2161 ggccggccct cgacctacgc gtcgatcatc tccaccatcc aggaccgggg gtacgtgcgg
     2221 ctggaggaga agcgcttctt ccccgaggac gtgggcgagg tcgtcacgga cctgctggtc
     2281 gagttcttcc ccgacatcgt ggacgtgaac ttcacctcca agatggagga ggagctcgac
     2341 gacatcgcgg agggcaagct cggctggatc caggtgctcg acgagttcta cggtcctttc
     2401 gagcgctcga tcgagaagaa cgtggaggag atccaacggt tcgaggagac gctggacgag
     2461 ctctgtccgc tctgccccga agagggacgt gagccggggc agctggtggt gaagctcggg
     2521 aagtacggca agttcatcgg ctgcaacaag tacccggagt gtcgatacat ccggaacctc
     2581 aacgggcagg agcgcaagga gcccgagatc ctggaggaga cctgtccgga gtgcggacgt
     2641 cctctggcca agaagtacgg gcggttcggg ccgttcgtgg ggtgcaccgg ctaccccgag
     2701 tgccggtaca tcaagaagca ggagcagaag accggcgtga ggtgcccgaa gtgcggatcg
     2761 ggcgagctgg tggtgaagcg ggcccggcgc ggcaagcgcg ggagcgtgtt ctacggctgc
     2821 aaccgctacc cggactgtga cttcacggtc ggtcagaagc cgctccccga gccctgtccc
     2881 agctgcggtt ccctgatggt ccagcagaag gacggcagcg ccaagtgcac cgcctgcggg
     2941 agcgtcgtgg agaccgtgga agcgtccgcc gcggaggcgt agttgtccca gctcgcggac
     3001 gaggtggcgc agctccgcgg cacccggtcc acctccttca aggatctcct cacgcacagg
     3061 ccgttcgcgc ggctgctggc ggcccagacc gtctcgtcgc tgggcgactg ggtgggcttc
     3121 gtcgcggtgg cctcgctggt gacccgactg gggggaacgg ccggagcctt cgccgtggcc
     3181 ggcgtgatga tggctcggat gctcccggcg atcctcttcg gtccgatcgc cggcgtcctg
     3241 gtggaccgct tcgatcgcaa gcggttgatg atcgtggccg atctgtcccg cggcgcgctc
     3301 tacgcgtcga tgcccttcct gggtcggctg tgggcgatct acctgctctc cttcgtgatc
     3361 gagagcctgt cgctgctgtg gacgccgtcc cgggacgcca gcctgcccaa gctggtcccc
     3421 cgtcggcagc tggcgaacgc caactccctc ggcctggtga ccacgtacgg gaccctgccg
     3481 ctcggggggc tggtgttcgc ggtcctggcc ggcgtctccg agctcttccc gtacgtgaag
     3541 cacgatcccg aagcggtgcc cttgctgctc gacggcggca ccttcctgtt ctccgcgctg
     3601 atggtccggg ggatccccat ccgcacgccc gcgagccgct ccctgggctc cttcgacctc
     3661 tcgcgcgcct ggggcgacgt ggtggacggg ttccggttcc tgcgcagcca tccgctggcg
     3721 tcctcgatga ccgtggggat cgtcacggcc ttcagcgcag tgggcgccgt tctggccatc
     3781 gggccggtct tcgttgcgac taccctgggg gcgaaggcgg ccggctgggg cgtgctggtc
     3841 acgtcgttcg ggatcgggat ggggatcggc atggcgctgg tcaacaagct cgtggagcgc
     3901 gtggagcggg acctggtctt cgtctggtcc atgatcgccg cggccgtcac cctgttcgtc
     3961 ctggcggcca tgccgaacct ggcgctggcg gccctgctgg cggtgttcct gggggtcttc
     4021 tgcggcgcca cctgggtctc gggctacgtg ctgctccagg agaacgtggc cgatgagtac
     4081 cggggtcgga ccttcggctc cctcaccgtg atgtcccgcc tcggcctgtt cctctcgctc
     4141 accgtgttcc cggtgatcgc gggcgccatc gggacccatc agctcttcat cggcggtcag
     4201 cgcatcgacc tgtccggcac gaggggcgcg atgtgggcgg ccgggatcat cgtgatcctg
     4261 gcggccgacg tcacccgcaa ggggctccgc cggttccggc tcagccgtcc caagccgctg
     4321 gctctcatgc cgaagctcaa gcgtccgccg gccacaggga ccttcatcgt gttcgagggg
     4381 gtcgagggcg ccggcaaggg cacgcagatc cggctggcga aggagtggct cgagtccgag
     4441 ggcgtggacg tgctggtgac ccgggagccg ggcggcaccg aggtgggcga gaagctccgc
     4501 gagctgctgc tcgatcacga gaccggtcac gtcgagcccc ggaccgaggc gctcctgttc
     4561 gccgcgagcc gctcccagca cgtggccacc gtgatccggc ccgcgctggc cgggggcaag
     4621 gtggtgatct gcgaccggta catcgactcc tccctggcgt atcagggatg ggctcggggg
     4681 ctgggggagc aggacgtcct caccctcagc gtctgggcca cccaggggct gttccccgac
     4741 ctcgtgatcc tgctccacat cgagccggag gccgggctgc tgcgctcgct cgaggagccg
     4801 gaccgcatcg agatggagtc tggcgacttc cattccaagg tcgccgacgc gtacctgaag
     4861 atcgccgagg agcaccccga gcgcttcgtg gtgatcgacg cggacgatcg ccccgaccgt
     4921 gtccacgagc aggtgaagca ggccctgagc cgggtgctgc gagagcgtga ggaggagcag
     4981 cagcaggagc ggccggagca ccatgagtga agccgcgccg ctcgccgacg ccgcaccggt
     5041 gctggaccgg gtgccggggc aggcatcggc catggcgttc ctgcatcggg ccacggcccg
     5101 tccccaccac gcctacctgt tcgcggggcc ggaggggggc ggcaagtcgc tcgccgcgcg
     5161 ggcgttcgcc gccgccctgc tgtgccgacg ccgcggctgc ggcacctgcc gcgactgccg
     5221 gctggcgctg gacgaccgtc accccaacga gttcctcgtg gagccggagg ggcgggacat
     5281 ccacgtcgac acgatccgga gcgaggtctg gcacccggcc caccgaacgg cacccgagcc
     5341 cggtcggaag gtgttcatca tccgggaggc ggaccggctg aacccggcgg ctgccgatac
     5401 cctcctcaag gtgctcgagg agcccccggc ggacgccgtc ctcatgctgc tctcggcccg
     5461 cccgcacgag ctgtccgaga cgatcctgtc gcgctgccac gtggtgacct tccaaccgct
     5521 ggcggaggga ttcgtggtgg agaccctcgc tgcggagggg gccgcgcccg agcgggcccg
     5581 gctggccgct cgcctctcgg gtggcaacct gggccgggct cggcggctgg cgatggatcc
     5641 ggaggggttg accttccgcc aggtggcggc ccaggccctc gagctcgccg cccggagcgc
     5701 tgccggtggc ctggacgcgg ccgatgcggt gctggcggcg gccgagcgct accgcaaggg
     5761 gctcaaggat cagctggaag aggagatcgc tccgttcctg gacgatcaag gccggccgga
     5821 ggacgcgtac cgtggggcca tccgacgcct ggaggagcga cacaggcgca gggtccgtcg
     5881 cgccgagcgc gactacgtgg actgggtcct gctggccatg tcctccctgc tccgggaccg
     5941 gatcgtggga gcggtgggcg gctccgacga cctgctgatg aacctggacc accccccggc
     6001 ggcctctgag gtgcgggcca gtgcgctggg cgtggcggcg gtggaggagg cccgcgcggc
     6061 gctggccgag gacctgaacc tcaacccgcg gctgctcctg gagcacgcct tcctcgccct
     6121 cggccggatc gcggcgtagg ccctgagggc cacgccgaaa gtggtgctgg catcactgtc
     6181 gtcgggcccg acccgtgaga gattcgggat ggaggtggca ggcgcatgag cgtccgggtg
     6241 ctgatcgtcg acgatcaaga gcccttccgg atggccgcgc gcatggtggt ggaggccgcg
     6301 gagggcttcg aggtcgtggg ggaggccgac accggcgagt cctcggtgga gatggcccgc
     6361 gagctcgatc ctgacctggt gctcatggac gtgaacctgc ctggggtcaa cgggctggag
     6421 gcgacccgcc ggatcctcga ggactcggcc gatcgcaccg tggtcctgct cctgtccacc
     6481 tacgaggagg aggagtacgc tccccgggcg gccgagtccg gggcggccgc gtacatcccc
     6541 aaggccgtgt tcggccccga tcgcctggaa gaggcctgga cccaggccgc cgacgctcgc
     6601 tgaggccgcg accagcgcat ccgggttccg gccgacctgc aagcagagcg cccgcgttct
     6661 tcgcaccttc aaccgtggtg cccgcatccc ctcgatgggg tgtaccggct ccgtcgaacc
     6721 gtggtggtct ccggctgttc cccgtggccc catcccacct accgtggtcc acacacggga
     6781 ggggtgagcg atgggacgga tcctggtggg aacggacacg agcccttcgg ccgatctcgc
     6841 cgttcgggcg gcagcggacc tggctcggca cacgcacgcg gagctcctgg tgctgtacgt
     6901 gcgctcggac ggagggctgc gagacgcggc ggatccacgg aagcgacccg acccgggccg
     6961 gtacatggcc gacctgtcgg ccaggttccc ggagcttcgg gtccggagct gggtcgagcc
     7021 cggcgagccg gctgaacggc tctgcgaggt ggccgtggac gaaggggtcg acacgatcgt
     7081 ggtggggaac aagggcgctg gcggttcgcg atggcgggtc cgggagagcg tgccgaccat
     7141 ggtcgtacgg cacgcgccgt gctccgtcct gatcgttgat acgagggtgg cccagtagat
     7201 gcccgcaccg gtgggggcat cccgagtctg gctacggcgt gcgctcgccc tcgcgcagga
     7261 aagcgagcac ggccatgacc cggcggtgga cgtcgggctc ctcggacagg tccagcttgt
     7321 ggaacaggga gttgatgtgc ttctcgaccg ctcgctccgt gaggaacaga gacttggcga
     7381 tcgccgcgtt gttcttgccc tgcgccatat gccccaggac ctcgcgctcc cgctccgtga
     7441 gctgcgcgag cggcgagtgg gccaggtgct ccttgcgcgc gaccagcgcc tccacgacct
     7501 tcggatcgag gaccgagccc cctctggcca cttcgtccag ggctcggacg atctctccca
     7561 gatcggccac ccgctccttc agcaggtagc ccaggccggc cgcgccgtcc ttcagcagct
     7621 cgtaggcgta ctcctcctcc gcgtactgcg agagcaccac cacccccacc tcggggtggc
     7681 gttcgcggat ccgcctggcg gcgtcgatcc cctccgtggt gttcgtgggg ggcatgcgga
     7741 tatcggtcag gacagcgtcg ggcctgtgcg cctccaccgc ctcgaggagt tcgtccaggg
     7801 tcgaggcggt ggccaccaga tccacctgct cggtggtctg cagcagcgcg gctgttccct
     7861 cgcggaccaa gtagttatcc tccgcgaaca ccacgcgtac catgcgcgaa tcgtacaggg
     7921 cgagggaggg gcggcggcgt gagcatccgg gtcgtgctgg gcgaggacaa cgtgctggtg
     7981 cgcgagggcg ttcgcgccct gctcgactcg taccaggacg tcgaggtggt gggggtggct
     8041 caggacgctc cttcgctcct gcagaccgcc gccgagcaca tccccgacgt cgtggtcacc
     8101 gacatcaaga tgccgcccaa cttccggctg gagggcatcg actgcgcgca cgcgatccgg
     8161 gacaagcacc cggatacggg tgtcgtggtc ctgtcggcgc acgatgacga ggagtacgcg
     8221 atcgcgcttc tggggaaggg gcagagcggc ctggcgtacc tgctgaagga tcggatcgcg
     8281 cagggagacg agctggtccg cgcgatccgg gaggtggccg cgggtggcag cgcggtcgat
     8341 cccaccatcg ccgagcgcct ctcggggcgc cacgacgccg aggaggacga ccggcggatc
     8401 ctggacatga tgtcccaggg cctcggctac gagcagatgg ccgagcgcct gggcaccacc
     8461 caggaggccg tggaccggca ggtcaccgag ctgttccgcc gcctggccgg gggggacggc
     8521 gggggtctgg tggacgagtt caagcgcctg cacgccgccg tggtggagca ggcgacctcc
     8581 cggcgcaccc tcgcctcctt cgtcccccag cagctcgcgg aacggctcgc gctgcagccg
     8641 gacgagccga tcgagccgca agaggtcgag gtcagcgtcc tcttctcgga catccggggc
     8701 ttctcgacga tcgccgagcg gctctcggcc cgcgacgtcg catccctcgt gggcaggcac
     8761 ctgtcggcga tggccgaggt cgtcgtggcc cacggcggca cgatcgacaa gttccagggg
     8821 gacgcggtaa tggccgtgtt cggagcgccc gatccccagc ccgatcacgc cgagcgtgcg
     8881 ttgcgctgcg cgatggcgat gcaggctcgc cagacagagc tggatgccca gggatggggg
     8941 gtcgacggac tcccgaccat gggcgtcggc atcggggtga atacgggacc ggtcatcgcc
     9001 ggcacggtcg gtgggggcgg gcgcctcgag tacacggtcg tgggcgacgc ggtgaacgtg
     9061 gcatcgcgcc tgcaatcgga ggctgaggcc ggagagatcg tggcgtccgc gtcgaccgtc
     9121 gaagctgccc ccgggatcac gtgtgagtcg atcgggcctc ggatcgtgaa gggtcgggaa
     9181 gagcccgtgg aggtgttccg tgtccgtcgc ggctgaactc gttcccgaac ggcccgcccg
     9241 gtggccctgg tacgcgttcc ccgccttcct cggcgccgcg ttcgtcggtc tcgtgctcgt
     9301 cgctcagaac gacgagcctc tcagccagca ggtcccgtac gtggtcgcgt tcacgatgtt
     9361 ctggctcgtg ggagccgtga tcgtgagccg cgtccctcgc aacacgatcg ggctgttcct
     9421 cctgtacacg tcggctgtga cggcgaccgc cttcctggcc agcgagctca cgacctggct
     9481 catcgagcgg ggtcatcgcg gacccggggt ggcagcgatc ggtctgctga acaaccttgc
     9541 ctggctgttc gggttcattc cggtcgtgtt catcctcccg ctcctgttcc cggacgggcg
     9601 gctcccttcg aagcggtgga agccctacgt gtggttcgtc gtcgcgttcc tcatgctctt
     9661 cgcgctcacc ctgctgttcg ggcagcgacg ggtgttcgga tcaggtagcg accccctcga
     9721 gatcccgaac cccttgtaca tccgggcgat cggtcgcctt ccggatctcg atccactgtt
     9781 cggtacgctg ttcccgctgc tgttcgccgt ctcggccgtc tccctcgtcc tccggttcca
     9841 ccggtccaag ggggtcgagc gacagcagat caagtgggtc gccttcgggc tgccggtcgc
     9901 gctggtgggg atcttcgtct ccaactactc ggaccccacc ctcgttggcg cggtgctcgg
     9961 cgccatcggg ttcctggcct tcccggtgtc gatcgggatc gccatccttc gcttccatct
    10021 ctacgacctc gacgtcgtcg tgcggaagac cgtcgtgtac gcggccctgg cgctgttcgc
    10081 caccctcgtg tacctggccc tggtggtggg cctcggggcc tggctcggcc ggggcagctc
    10141 gctcctgacc atcgtggccg cggtcgtggt ggccgcggcg ttccagcctg tcagggctcg
    10201 actgaaccgg ttcgcgaacc ggc
//
LOCUS       DASQQQ010000234         4268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_400550, whole genome shotgun sequence.
ACCESSION   DASQQQ010000234 DASQQQ010000000
VERSION     DASQQQ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400550"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VE646_09015"
     CDS             complement(<1..973)
                     /locus_tag="VE646_09015"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80165.1"
                     /translation="MDAAGILLAGAMVALAFASERGDPWPLVGLVLAVAAALVAGRVL
                     GWIHRGIAPACLVLAGVVLIAREQGLVGAGPLSGPFGYRNATAAFFAQAAVAALVLGA
                     AVRRPALWAGAGVVAVGFAWVAMRDSAAAAGTLVLVVLALLAVRGARGAGLATAVAGL
                     AFVAVLGGTIALGAAYAPGDGGPAARALTERRLVLWHESLDLMVRHPGGVGPGRFAQV
                     DRTARRDPDAGWAHQEFLQQGVELGWAGFALTVLLFVWGFARLLVHPAPDGVVALGAV
                     ALAALGIHAGIDHILHVAAVPVAAAALLGTAQAVPHRRSERVHHHARQ"
     gene            complement(1027..2388)
                     /locus_tag="VE646_09020"
     CDS             complement(1027..2388)
                     /locus_tag="VE646_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80166.1"
                     /translation="MQTRRAEISPRAVPAAIVIGLGLVAGYIVLLLVLMNEGSYDAWA
                     VMLLVPLMMLVSLPVLRRQAAREGDPRVFKLLTLALLVKLGGSIARYYVAFSLYGGVA
                     DATGYHNAGVKLFEQFRHLDFAPLLHATGTEFLRVFTGLVYAIIGPTRLGGFLFYAWL
                     GFWGLFLFYRAFVVAVPEGRRRSYAILLFFLPSLVFWPSSIGKESWMMFALGIATLGI
                     AIALSGQLWRGLAMAAVGFWLAGIVRPHIAALLGAAFALAMVARRGSRELHELAPVMK
                     ALSMAVVAVLALFLTVKAERFLQRSGIPTSKGVSATLTDIQDRTSKGGSDFVPSVVDS
                     PARAPLAVVTVLFRPFLPEAHNLQARLAAVEGTFLILLCLFRWRWLIAAIRSIRGSPY
                     VLFALVYTGLFIIAFSSFANFGLLARERVQLYPLFLVLLCIPPAVREQARSGAEAAGA
                     AIT"
     gene            complement(2388..3416)
                     /locus_tag="VE646_09025"
     CDS             complement(2388..3416)
                     /locus_tag="VE646_09025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZD80167.1"
                     /translation="MSGTVSHMDILVRPCQAQDEDEVLRLLRASLGGGPAGNRSAEFF
                     RWKHVENPFGPSFMLVAETDERIVGLRAFMRWRFLAGERELRAVRAVDTATHPDFQGR
                     GIFRRLTLDALDQLRDQTDLIFNTPNDKSLPGYLKMGWESVGDLPIRVRVARPVRFAR
                     GVRSWRSLETAAAGETLTHARTAEQALDEPGIPGLLAEAERRDAIATVRGLAYLRWRY
                     ARAPLLDYRGVTARDGMSVRGLGLFRVRPRGALTEATVSEVLVRRGDVGGARAVLQAI
                     RRSAGVDHLTCSFPQGSTALRAARVAGYLRAPGGVRLVTNALGHRLEPDPRRLPSWAL
                     SVGDVEVF"
     gene            complement(3413..4228)
                     /locus_tag="VE646_09030"
     CDS             complement(3413..4228)
                     /locus_tag="VE646_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZD80168.1"
                     /translation="MARARVGLRSVTSSVRPLPSFLIIGAQRAGTSSLYKYLEGHPGL
                     AASVRKETEYFSRRYGRGETWYRAHFPLLRGRLAYEATPDYLFYPLSPERIRKTIPDA
                     RFVVLLRDPVERAYSHYRHMVRLGFEDLPFGEALTREPERLSGDAARLADDPLYEPRA
                     FLRFSYVSRGLYAEQFRRWFSWFPPSRFLVIQSERLFEDPKRAIVEIEQGIGARPWLP
                     RDLRNHSYGTQRPPVPPIGGAERHMLQERLRPEVQELERLLGRSFDWKLGDGG"
BASE COUNT          768 a         1577 c         1412 g          511 t
ORIGIN      
        1 cctggcgagc gtggtgatgg acacgttccg acctccgatg cgggacggcc tgtgccgtcc
       61 ccagtaaggc ggccgccgca acggggacgg ccgccacatg caggatgtgg tcgatcccgg
      121 catggatccc cagggccgcc agggccaccg cacccagggc caccacgccg tcgggcgccg
      181 gatggaccag cagccgggcg aagccccata cgaagagcag gaccgtgagg gcgaacccgg
      241 cccagcccag ctcgaccccc tgctggagga actcctgatg cgcccacccg gcgtccggat
      301 cgcgccgcgc ggtccgatcc acctgggcga accggcccgg acccacgccc cccgggtgcc
      361 gaaccatcag gtccaatgac tcgtgccaga gcaccagccg ccgctcggtg agcgcgcggg
      421 ccgcgggacc cccgtcaccg ggtgcgtacg ccgcgcccag cgcgatggtt ccgcccagca
      481 cggccacgaa cgccagaccc gccaccgccg tggcgagtcc ggccccacga gccccccgca
      541 ccgcgaggag ggcgagcacg accagcacca gcgtgcccgc ggcggcggcc gagtcccgca
      601 tcgccaccca ggcgaacccg accgcgacca cacccgcccc cgcccagagg gctggccggc
      661 gcacggccgc gccgagcacc agcgccgcga cggcggcctg cgcgaagaac gccgccgtgg
      721 cgttgcggta cccgaacgga cccgacagcg gcccggcccc caccagcccc tgctcgcgtg
      781 cgatcagcac caccccggcg aggaccaggc acgccggcgc gatcccgcgg tggatccacc
      841 ccaggacccg accggcgacc agggccgccg ccacggccag gaccagcccc accagcggcc
      901 acggatcacc ccgctccgaa gcgaacgcca gcgccaccat ggcgcccgcc agaaggatgc
      961 cggcggcgtc catggccgtg cgggttcgcc gacccgatcc ttcgaagggg cgggaccccg
     1021 gtccgttcac gtgatcgcgg ccccggctgc ctcggccccg gaacgagcct gctcacggac
     1081 ggcgggcggg atgcacagca acaccaggaa cagggggtac agctggaccc gctccctggc
     1141 cagcaggccg aagttggcga agctggagaa ggcgatgatg aacagcccgg tgtagaccag
     1201 cgcgaacagc acgtagggcg agccccggat ggaacggatc gccgcgatca gccatcgcca
     1261 ccggaacaag cacagcagga tcaggaaggt gccctcgacc gccgccaggc gcgcctgcag
     1321 gttgtgcgcc tcgggcagga acggcctgaa cagaaccgtc acgaccgcga gcggtgctcg
     1381 agccggcgaa tcgacgaccg aggggacgaa gtccgagccc cccttcgacg tccggtcctg
     1441 gatgtcggtc agggttgcgc tcaccccctt ggaggtcggt atgcccgacc gctggaggaa
     1501 acgctcggcc ttgacggtca ggaacagggc aagcacggcc accaccgcca tcgacagggc
     1561 cttcatcacc ggtgccagct cgtgcagttc gcggctcccc cgacgtgcca ccatggccag
     1621 tgcgaaggct gcacccagca gcgcggctat gtggggccgc acgatcccgg ccagccagaa
     1681 gcccaccgcc gccatcgcga gccccctcca caactgcccg gacagcgcga tggcgatccc
     1741 caaggtggcg atccccagcg cgaacatcat ccaggactcc ttgccgatgc tcgagggcca
     1801 gaacaccagc gagggcagga agaacaggag gatcgcgtac gagcgccgcc gcccttcagg
     1861 tactgccacc acgaacgctc gatagaacag gaacaggccc cagaacccca accacgcgta
     1921 gaagaggaac ccaccgagcc tggtggggcc gatgatcgcg tacacgaggc cggtgaacac
     1981 tctgaggaac tcggtccccg tcgcgtggag gagcggggcg aagtccaggt gtcggaactg
     2041 ctcgaacagc ttcacgcccg cgttgtggta gccggtcgcg tcggccacgc cgccgtagag
     2101 gctgaacgcg acgtagtacc gggcgatcga gccccccagc ttcaccagca gcgcgagcgt
     2161 cagcagcttg aagacgcgcg gatcgccctc tcgcgctgcc tgacgcctca ggaccggcag
     2221 cgagaccagc atcatcagcg ggaccaggag catgacggcc caggcgtcgt acgagccctc
     2281 gttcatgagg accaggagca ggacgatgta accggcgacc aagccaaggc cgatgacgat
     2341 ggccgccggg acggccctcg gcgagatctc cgcacgcctg gtctgcatca gaagacctcc
     2401 acgtcaccga cggacagcgc ccacgagggc aaccgacgcg gatccggctc aaggcgatgc
     2461 cccagcgcgt tcgtcaccag cctgacccca cccggcgccc gcaggtagcc ggccaccctc
     2521 gccgctcgaa gcgccgtgga cccctggggg aagctgcacg tgaggtggtc aacaccggcc
     2581 gatcgtcgta tggcctgcag caccgcccgg gctcctccca cgtcgccccg ccggacgagt
     2641 acctcggaca cggtggcctc ggtgagagct cctctcggac ggaccctgaa gagcccgagt
     2701 ccacggaccg acatcccgtc gcgcgccgtc acgccccggt agtccagcag cggcgcccgc
     2761 gcgtaccgcc accgcaggta cgcgaggcct cgcaccgtgg cgatcgcatc cctgcgctcc
     2821 gcctctgcca gcagcccggg gatgccgggc tcgtcgaggg cctgctccgc ggtccgggcg
     2881 tgggtgagcg tctcccctgc cgcggccgtc tcgagcgagc gccacgagcg gaccccccga
     2941 gcgaaccgca cgggtcgagc aacccgcacc cggatcggca ggtcgcccac cgactcccat
     3001 cccatcttca ggtagccggg caggctcttg tcgttcgggg tgttgaagat cagatccgtc
     3061 tggtcacgaa gctggtccag tgcgtcgagg gtgagccgtc ggaagatgcc gcggccctgg
     3121 aagtcggggt gggtcgccgt gtccaccgcc cgcaccgcac ggagctcacg ttcgcccgcc
     3181 aggaacctcc atcgcatgaa ggcgcggagt cccacgatcc gctcgtccgt ctccgccacc
     3241 agcatgaacg aaggcccgaa ggggttctcg acgtgcttcc accggaagaa ctccgccgac
     3301 cggttccccg ccgggcctcc tccgagggac gcacggagga gtcgcaggac ctcgtcctca
     3361 tcttgcgctt ggcaagggcg gacgaggatg tccatgtggg agaccgtccc gctcatcctc
     3421 catctcccag cttccaatcg aacgaacggc cgagcagtcg ctccagctcc tggacctccg
     3481 gacggagccg ctcttggagc atgtgccgct ccgccccgcc gatgggtggc acgggcggac
     3541 gctgggtgcc gtagctgtgg ttgcgaagat ccctcgggag ccaggggcgc gcaccgatcc
     3601 cttgctcgat ctccacgatc gcgcgcttcg gatcctcgaa caagcgctcg ctctggatca
     3661 ccaggaacct cgatggaggg aaccaggaga accaccgccg aaactgctcc gcgtacagac
     3721 ctcgggagac gtaggagaag cgcaggaagg cacgcggctc gtagagcgga tcgtcggcga
     3781 gccgggcggc atcccccgac aaccgctcgg gttcccgtgt gagagcttcg ccgaagggaa
     3841 gatcttcgaa gccgaggcga accatgtgtc ggtagtgcga gtaggcgcgc tccaccgggt
     3901 cccggagcag aaccacgaac cgcgcgtccg ggatcgtctt ccggatccgc tcgggactga
     3961 gcgggtagaa gaggtagtcg ggggtcgctt cgtacgcgag ccgtccacgg aggagcggga
     4021 agtgggcgcg ataccaggtc tcccctcgtc cgtacctcct ggagaagtac tccgtctcct
     4081 tcctcaccga ggcagccaaa cccggatgac cttccaggta cttgtagagg gaagaggtgc
     4141 ctgcccgctg ggcgccgatg atcaggaagg aaggcaacgg gcgtacagag ctcgtcacgc
     4201 tccggaggcc gacccgagcc cgggccacct tggatggaac cccacgcggc aatcggctct
     4261 tgatccct
//
LOCUS       DASQQQ010000235         3300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_371576, whole genome shotgun sequence.
ACCESSION   DASQQQ010000235 DASQQQ010000000
VERSION     DASQQQ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371576"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VE646_09035"
     CDS             complement(<1..700)
                     /locus_tag="VE646_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018587875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HZD80169.1"
                     /translation="MTFEHGKLAMQAGGSVVVTLGETQVLATTTSSAPREGIDFFPLT
                     VDVEERMYAAGKIPGGFFRREGRPSETAILTARLTDRPMRPTFKEGYRDEVQVVVTVL
                     SVDMGNPYDIPSINAASLATMIAGLPFEGPVGAVRMGLIGGEWKIDPTFQDLEQATFD
                     VVVAGRRNDAGGIDVLMIEGEAPDNTWDLLSNGGASAAPTEEVVAEGLEAAKRAIGEM
                     IDFQNEFLSQIGVEP"
     gene            complement(958..1227)
                     /gene="rpsO"
                     /locus_tag="VE646_09040"
     CDS             complement(958..1227)
                     /gene="rpsO"
                     /locus_tag="VE646_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HZD80170.1"
                     /translation="MALTKEAKSQIISEYRRSDGDTGSAEVQVAVLSNRISGLTEHLK
                     THKKDHHSRRGLLQMVGRRRRLLEYLKREDIERYRELIGRLGLRR"
     gene            complement(1277..2008)
                     /locus_tag="VE646_09045"
     CDS             complement(1277..2008)
                     /locus_tag="VE646_09045"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZD80171.1"
                     /translation="MHPLSGSAIVFLLLAVACGAVIQGSVGFGYALVTVPALALVEPP
                     AVPATALLLAAPLTILMALRERRHIHVRGYLSIAAGRILGTVIGVWVIVSIPTGELSV
                     LVGALIMGIVVMSIVRLDLQPTTPVAFGAGFVSGITGTAVAIGGPALAMVYQHRPGPE
                     LRSTLAVSFVTGLTLSLSGLFLAGRVQRQHVVLALELVPGLLAGLWLSGFVARAVDAR
                     WLRPAVLTFAGLSGALIVTRGILGR"
     gene            complement(2008..3096)
                     /locus_tag="VE646_09050"
     CDS             complement(2008..3096)
                     /locus_tag="VE646_09050"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HZD80172.1"
                     /translation="MLPRHRRRSHVPFAVPLVALVLIVAAVVGFRLGDRSGAAASGAF
                     AGGASPSGAALALEGSAGGARQGAPSPRATPSPSRGRLVIHGTGDVSLDPSYLPVFGS
                     RGYGWAWSGLDGLFRRDDLTLINLECPATHVSAPVSKQFVFRCDPAALPAAKAAGVEV
                     ANQSNNHAFDQGAQGLLDSLKQIRRAGLVPVGAGANQAQALRAVLFELNGWRVAVVGI
                     DEVIDPEYEVAGPDSPGTAAGHDFPLALQAVRDAARRSDLVVVMIHWGVELDARPRDY
                     QVQEGHRLIDAGADVIYGGHSHRLQPMETYRGHPIFYSLGNFVWPRYSAGGSTTAVAE
                     VTVSTKGRIRGRLLPASIASDGHPVLTG"
     gene            complement(3101..>3300)
                     /locus_tag="VE646_09055"
     CDS             complement(3101..>3300)
                     /locus_tag="VE646_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HZD80173.1"
                     /translation="FGVEPLHVEAFLLGFDDTDLRGGALSVEFWQRLRDEERFGTTEE
                     LVRAMEEDVRRTRRIVPAVGG"
BASE COUNT          494 a         1225 c         1153 g          428 t
ORIGIN      
        1 tcggctccac gccgatctgg gacaggaact cgttctggaa gtcgatcatc tcgccgatcg
       61 cgcgcttggc cgcctccagg ccctcggcca cgacctcctc ggtgggggcc gccgaggcgc
      121 ccccgttgga gagcaggtcc caggtgttgt cgggggcctc gccctcgatc atcagcacgt
      181 cgatcccccc ggcgtcgttc cgacggccgg ccaccaccac gtcgaaggtg gcctgctcca
      241 ggtcctggaa ggtggggtcg atcttccact cgccgccgat cagacccatc cgcaccgcgc
      301 ccaccggccc ctcgaagggc aggccggcga tcatggtggc caggctggcg gcgttgatgc
      361 tggggatgtc gtaggggttg cccatgtcca cggataggac ggtcaccacg acctggacct
      421 cgtcccggta gccctccttg aaggtggggc gcatgggccg gtcggtcagg cgcgcggtga
      481 ggatcgcggt ctcggaggga cggccctcgc ggcggaagaa cccgccgggg atcttgccgg
      541 ccgcgtacat ccgctcctcc acgtccacgg tgagcgggaa gaagtcgatg ccctcgcggg
      601 gcgccgagct ggtggtggtg gcgagcacct gcgtctcgcc gagggtcacg accacggatc
      661 cgcccgcctg catggcgagc ttgccgtgct cgaacgtcat ctgctgggac ccgacggtcc
      721 tgctgagctt ggtggtggcc actggcctct cctccttggg gaacggcccg cacgcccggc
      781 gcgcgggaac ctccggtggg tcgtctggag gtggggaggc tcggccagtt gtcagtggtg
      841 agccaccggg aaccgcgggg gtctcgggag ctcgctactg agaaccgacc cgatgcgctc
      901 ccctcctccg ttggggcttc gtcgtccttg ggacatccct cctcgcggga aggggcgtta
      961 ccggcgcagg ccgagccggc cgatcagctc gcggtagcgc tcgatgtcct cgcgcttgag
     1021 atactcgagc agccggcggc ggcggcccac catctggagg agcccgcggc gcgagtggtg
     1081 atccttcttg tgggtcttca ggtgctcggt cagaccgctg atccggttcg acaggaccgc
     1141 aacctgcacc tctgccgacc cggtgtcacc gtcgctgcgg cggtactcgg agatgatctg
     1201 ggacttcgct tccttcgtca gtgccatatc cagcgggaag gctaccagac agggtgtccg
     1261 aacactccct cgtcgctcat cgcccgagga tcccgcgggt cacgatcagg gcacccgaca
     1321 gccccgcaaa cgtgagcacc gccgggcgca gccaccgggc gtcgacggcc cgggccacga
     1381 accccgagag ccacaggccc gccagcaacc ccggcaccag ctccagggcc aggaccacgt
     1441 gctggcgctg cacgcgcccg gccaggaaca ggccgctcag ggacagcgtg aggccggtca
     1501 cgaacgacac cgccagcgtg gagcggagct cgggcccggg ccggtgctgg tacaccatgg
     1561 ccaaggccgg cccgccgatc gccaccgcgg tgccggtgat ccccgagacg aacccggcgc
     1621 cgaaggcgac cggggtggtg ggctgcaggt ccagccgcac gatcgacatc accacgatcc
     1681 ccatgatcag cgcccccacc agcaccgaca gctcgccggt ggggatcgac acgatcaccc
     1741 acaccccgat caccgtgccc aggatccggc cggcagcgat cgacaggtag ccgcgcacgt
     1801 ggatgtgccg gcgctcccgg agcgccatca ggatcgtgag cggcgcggcc agcagcaggg
     1861 ccgtggccgg caccgccggc ggctccacca gcgccagcgc ggggaccgtc accagcgcgt
     1921 agccgaagcc caccgagccc tggatcaccg ctccgcacgc cacggcgagc agcaggaaca
     1981 cgatggccga gccggagagg gggtgcacta ccccgtcagc accggatggc cgtcggacgc
     2041 gatcgaggcg ggcagcagcc ggccccggat ccgccccttc gtcgagactg tcacctcggc
     2101 caccgcggtg gtggaaccac cggccgagta ccggggccac acgaagttac ctaggctgta
     2161 gaagatcggg tgcccgcggt aggtctccat gggctggagg cggtgcgagt ggccgccgta
     2221 gatcacgtcg gcccccgcgt cgatcagccg gtgcccctcc tgcacctggt agtcgcgggg
     2281 ccgcgcgtcc agctccacgc cccagtggat catcaccacg accaggtccg agcggcgcgc
     2341 cgcgtcccgg accgcctgca gcgccagcgg gaagtcgtgc ccggccgcgg tccccggcga
     2401 atcggggccg gccacctcgt actcggggtc gatcacctcg tcgatcccca ccaccgccac
     2461 ccgccagccg ttcagctcga acagcaccgc acgcagcgcc tgggcctggt tcgcgccggc
     2521 ccccaccggc accaggccgg cccggcggat ctgcttcagc gagtcgagca gcccctgggc
     2581 cccctggtcg aaggcgtggt tgttcgactg gttggccacc tccacgccgg cggccttggc
     2641 ggccgggagg gccgccgggt cgcagcggaa gacgaactgc ttgctcaccg gcgcggagac
     2701 gtgggtggcg gggcactcca ggttgatcag ggtcaggtcg tcgcggcgga acagcccgtc
     2761 cagacccgac caggcccagc cgtagcccct ggacccgaac accggcaggt agctcgggtc
     2821 caggctcacg tcaccggtcc cgtggatcac caggcgcccc cgcgacggcg atggcgtggc
     2881 cctgggcgag ggagcccctt gccgggcccc gccggccgag ccctccaggg cgagcgccgc
     2941 ccccgacggc gaggcaccgc cggcgaacgc gcccgaggcc gccgcgcccg agcggtcgcc
     3001 caggcggaag cccaccaccg ccgccacgat cagcaccagg gccacgaggg gaacggcgaa
     3061 gggcacgtgg gagcgacggc ggtggcgggg gagcacggcg ctatcctccc acggcgggca
     3121 cgatcctccg ggtacgccgg acgtcctcct ccatggcccg caccagctcc tcggtggtgc
     3181 cgaagcgctc ctcgtcccgc aggcgctgcc agaactcgac gctgagcgct ccaccgcgca
     3241 ggtcggtgtc gtcgaagccc agcaggaagg cctccacgtg cagcggctcc acgccgaagg
//
LOCUS       DASQQQ010000236         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_371687, whole genome shotgun sequence.
ACCESSION   DASQQQ010000236 DASQQQ010000000
VERSION     DASQQQ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371687"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VE646_09060"
     CDS             complement(<1..459)
                     /locus_tag="VE646_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015658712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HZD80174.1"
                     /translation="MTTGRPVRPSSRGRGIPEATVARLPVYLRALTALSERGVLTVSS
                     EELATAAGVNSAKLRKDLSYLGSYGTRGVGYDVEYLVYQISRELGLTQDWAVVIIGIG
                     NLGHALANYGGFASRGFRVAALIDADPARTGETVAGLGVRHLDDLEQVIQD"
     gene            complement(1003..1254)
                     /locus_tag="VE646_09065"
     CDS             complement(1003..1254)
                     /locus_tag="VE646_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019888498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HZD80175.1"
                     /translation="MSAAAGGHTITLIGKPGCHLCEDARAVIARVAGELGASWEELDI
                     TQDEELYRRYWEQIPVTLIDGEQHDFWRVSEQRLRAALG"
     gene            complement(1251..>2102)
                     /locus_tag="VE646_09070"
     CDS             complement(1251..>2102)
                     /locus_tag="VE646_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018347640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZD80176.1"
                     /translation="VYTGATGVLADRFDPAGTLAEIRRTGVTNIPGAPPMYAAWTQQP
                     EAGEVLAGVRLMISGASPLPPVVLEQLRTSVGRPIYEGYGLTETAPVVASTLASGEAK
                     PGSVGRPVPGVELKLVDAFGDETHEDDPGEIVIRGANLFSGYWPDGSGGPDEEGWFRT
                     GDVAYADEDGDLYLVDRRKELVLVNGFNVYPREVEDVIAEHPAVAESAVIAVPHPFTG
                     EAVKALVVPKKGAQLTPEDVTAHCATRLARFKCPTVVQIVPELPHSATGKVAKGRLRA
                     RERERSG"
BASE COUNT          293 a          762 c          742 g          305 t
ORIGIN      
        1 gtcctggatc acctgctcga ggtcgtccag gtgccgcacc ccgaggccgg ccacggtctc
       61 gccggtgcgc gccgggtcgg cgtcgatgag cgccgcgacc cggaagccac gggaggcgaa
      121 cccgccgtag ttggccagcg cgtgcccgag gtttccgatg ccgatgatca cgaccgccca
      181 gtcctgggtg aggcccagct cgcgggagat ctggtacacg aggtactcga cgtcgtagcc
      241 caccccgcgg gttccgtacg agccgaggta ggacaggtcc ttgcggagct ttgcggagtt
      301 gaccccggcc gcggtggcca gttcctcgga tgagacggtg aggacaccgc gctcggagag
      361 cgccgtgagc gccctgaggt agaccgggag cctggcgacc gtcgcctcgg ggataccgcg
      421 tccccggctg gacggtcgga ctggacggcc tgtggtcacg ggactcctga cgcgctgaga
      481 gaggactcgc ctccccgtac cggagagggg gacctcaccg atgcgacctg gcttgtgctt
      541 ggcaagggta ggcgtttgtg aacgtacgca caaagtcgag accgtgcgat cacgtacggt
      601 ggtgaccgaa gtcacgcctg tcctgccgtc atcggcctac cgcccggtag gggaagtgcc
      661 tgcgcaaagg cccggggcgg gcgttcggac gacggcccgg aggacgtcct ggcgcgaacc
      721 aatgggtatc tcgacgcggg gtgaccgggg gccggggccg ggggatcgcg ggtgcgagga
      781 ccgcggcggg cagccgcggc tccccgtccc ctggtgagcc gatcgcgtgc ctcgccgcgg
      841 tcgtcgcgtg cggcggcagt gcgtcccccc gggcgcatgc cgcacgggtg tgccccgcgc
      901 ccgcaaaccc gaccttcgtc ggcggaccgg aacgaaccgg caactcccgg tgaacaccgg
      961 gcgaacaggg tcggaaagtc cagcgcggcc gagttaccgc ggtcagccga gcgcggcccg
     1021 cagccgctgc tcggacaccc gccagaagtc gtgctgctca ccgtcgatca gggtgaccgg
     1081 gatctgctcc cagtaccgcc ggtacagctc ctcgtcctgg gtgatgtcca gctcctccca
     1141 gctcgccccg agctcgccgg ccacccgggc gatcaccgcg cgggcgtcct cgcacaggtg
     1201 gcaccccggc ttgccgatca gggtgatggt gtggccaccg gccgcggcgc tcacccgctc
     1261 cgctcccgtt cccgcgcgcg aagccggccc ttcgcgacct tgccggtggc cgagtgcggc
     1321 agctcgggca cgatctggac gaccgtcggg cacttgaacc tggccagccg ggtggcgcag
     1381 tgcgcggtca cgtcctcggg cgtgagctgc gcccccttct tcggtaccac cagcgccttc
     1441 accgcctcgc cggtgaacgg gtgcggtacc gcgatcaccg ccgactccgc caccgccgga
     1501 tgctcggcga tcacgtcctc gacctcgcgg ggatatacgt tgaagccgtt gaccagcacc
     1561 agttccttgc gcctgtcgac caggtacagg tcgccgtcct cgtccgcgta ggcgacgtcg
     1621 ccggtccgga accagccctc ctcgtccggg ccgccggacc cgtccggcca gtaaccggag
     1681 aagaggttgg cgccccggat cacgatttcg cctggatcat cctcgtgcgt ctcgtcgccg
     1741 aaggcatcca ccagcttcag ctccacaccc gggaccggac ggcccactga tccgggcttg
     1801 gcctccccgg acgcgagcgt ggacgcgacc accggtgccg tctcggtcag cccgtacccc
     1861 tcgtagatgg gccgcccgac gctggtcctg agctgctcga gcaccacggg gggcagcggg
     1921 gatgcgccgg agatcatcag ccgcacgccc gcgagcacct caccggcctc cggctgctgg
     1981 gtccaggcgg cgtacatcgg cggcgcgccg gggatgttgg tgacgccggt gcggcggatc
     2041 tcggcaagcg tcccggccgg gtcgaaccgg tccgccagca ccccggtcgc gccggtgtac
     2101 ac
//
LOCUS       DASQQQ010000237         4887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_43688, whole genome shotgun sequence.
ACCESSION   DASQQQ010000237 DASQQQ010000000
VERSION     DASQQQ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43688"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="VE646_09075"
     CDS             complement(<1..166)
                     /locus_tag="VE646_09075"
                     /inference="COORDINATES: protein motif:HMM:NF040429.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6457 domain-containing protein"
                     /protein_id="HZD80177.1"
                     /translation="MEAWIDQLAEALGEDTLSERETSMILTAARDVAHRVERRLTPVA
                     AFLLGSAVGRS"
     gene            complement(506..1084)
                     /locus_tag="VE646_09080"
     CDS             complement(506..1084)
                     /locus_tag="VE646_09080"
                     /inference="COORDINATES: protein motif:HMM:NF019413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HZD80178.1"
                     /translation="MARDAATLVERHGWNADVHGISSSLHMTPLRIAPAVEERLAELS
                     ARYERVIVVYGDCGTGGRLDEVLARYPAARPAGVHCFQWYAGKLYRRFSQDIGIYFLT
                     DWLVDNWDRAVVHGLGLDRFPWLKETYFGNLTRILFVRQHPDRDREAAARSIAAWMDR
                     PLEIHDLGIEPLDELLSPLMGPHAEEGADLRP"
     gene            complement(1126..2958)
                     /locus_tag="VE646_09085"
     CDS             complement(1126..2958)
                     /locus_tag="VE646_09085"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HZD80179.1"
                     /translation="MRKLEVTYLPQGATVHVPMGTNLFHAAHWAGLPIESTCGGRGTC
                     GKCRVRVMGGRGEPTTADQRHLSSAELEAGWRLACQAEIRADTVCEVPRPMRTPKAAT
                     MGVGRFVLLEPNVRRIVLPMEAPTLDDARDHLRRIREALADEGLEVSWDPSLLPRLAA
                     ALSEGSEVTATVVGDRLVDVEPGDAADRRFGISLDIGTTTVVATLVDLVSGNATAVES
                     TINRQAPFGADVIARMGHAMTGPEALDDLRAAVLATVNGLVESVCATAGVELEAVLEM
                     VVVGNATMLHLLLGVDPRSVALSPFVATFLQAQDLRARDVGIQLHPEGRVVLLPSIGA
                     YVGADIVGDVVATGLAREDRMRLLVDVGTNGEIVCGSVDRVVATAAPAGPAFEGGQIL
                     HGMRATEGAIEGVTFGPSGIRLQVIGGDVQPRGICGSGLIDAVAQLRVSGLLKENGIL
                     LSWEEATVAEHPLADRLVDRDGIRAFALTDEVLLTQLDIRELQSAKGAIATGIEVALV
                     ALGIDPRDLDEVLLAGSFGTYIDPDGARIIGLVPPVPVERIRAVGNTASEGAKMALMS
                     FREREMALELPELIEYLELSGAPGFNERFIRNLAFPSLEAVRSS"
     gene            complement(2955..3827)
                     /locus_tag="VE646_09090"
     CDS             complement(2955..3827)
                     /locus_tag="VE646_09090"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZD80180.1"
                     /translation="MATILEGPGGPVYIGDDHPFVVIGERINPTGRPRLTEELVAGVM
                     DTVRADAEAQVRAGARVLDVNAGIPGFDEAAMLAQVVRAVQEVVNVPLCIDSSTPEVL
                     EASIAASRGKVIVNSVTAEEASLSRLLPVVGRHGAAVIGMAHDETGISMDPERRLAAA
                     RTIVEAAADHGIVREDVIIDPLAMPIGAEPRAATAMLETIRAIRRELGVNVSCGASNL
                     SFGMPGRDHIDAAFLTMAILTGMNVAITNPLHDPVRKAFLAADLLLGRDAHGAAWIAA
                     HRAAMASTGQGPAA"
     gene            complement(3840..4478)
                     /locus_tag="VE646_09095"
     CDS             complement(3840..4478)
                     /locus_tag="VE646_09095"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HZD80181.1"
                     /translation="MEQDLQTLRRALISADRTTAVDTARRLAGSGLDPRTILEAGMAV
                     AMFELGEMWKRGEVFLPEVVASAEIFKQCNEIVEPALLERREENDRSLVLLATVKGDL
                     HDLGKNMVAAMLRTSGFEVHDLGKDVPADRIVEAIREHGPRIVGLSALLTTTVPYQET
                     VIRALERDGLRGAVKVMVGGAPVTPGWAEKIGADGYANNAPEAVEVAKGLIG"
     gene            4693..>4887
                     /locus_tag="VE646_09100"
     CDS             4693..>4887
                     /locus_tag="VE646_09100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZD80182.1"
                     /translation="MPDVIIVGAGITGTSIAFHLATRSSLGVLLVDRAGVAEGGTGRS
                     SALIRMHYTFPPEVRLALRSL"
BASE COUNT          703 a         1804 c         1660 g          720 t
ORIGIN      
        1 tgctccgccc caccgctgag cccagaagga aagcggcgac gggggtcagc cggcgctcga
       61 cccgatgcgc gacgtcccgg gccgcggtga ggatcatgct cgtctcccgt tcgctgagcg
      121 tgtcctcgcc cagcgcctcc gcgagctggt cgatccaggc ctccatcgat ccctcctcgg
      181 tccggctgcc gtcatcacgt tagtacggag gcgggagctc ggctccgtcc tccggctcgt
      241 gcctccgcct ccaggaccgg ctcataactc cggctcggga ggctcgggcc ggcgcctggc
      301 catgtgggcc tgcagctcct cctcgatggc gaggtccagg ccgcccggcc cgtctcgggg
      361 atccgcaaga gctccaggtc cagcgcgaac ttctcgaacg aggcggtcag ccctccctcg
      421 agccacccgg cgcccttgcc caccatctcc tccacggtcg tgcacggcgt gcagctgctc
      481 ctcggacaag acccgcggag ccccttcacg ggcgaagatc ggcgccctcc tccgcgtgcg
      541 gccccatcag cggggacagc agttcgtcca ggggttcgat ccccaggtcg tggatctcca
      601 gcgggcggtc catccaggcc gcgatggacc gagccgcggc ctcccgatcc cggtctgggt
      661 gctggcggac gaacaggatc cgcgtgaggt tgccaaaata cgtctccttc agccacggga
      721 accggtccag accgagcccg tgcaccacgg ctcggtccca gttgtccacc agccagtcgg
      781 tcaggaagta gatcccgatg tcttgggaga acctccggta gagcttgccc gcgtaccact
      841 ggaagcagtg gaccccggcc ggacgggccg ccgggtaccg ggccaggacc tcgtccagac
      901 gccctccggt cccgcagtcg ccgtacacga cgatcacccg ctcgtagcgc gcgctcagct
      961 cggccagccg ctcctccacg gcgggggcga tccgaagggg cgtcatgtgg agggagcttg
     1021 agatgccgtg gacgtccgcg ttccagccgt gccgctccac gagggtcgcg gcgtcccgcg
     1081 ccacggctcc acaggccaag agggcaaccg gagcgcccct ggccctcacg aggaccgcac
     1141 cgcctcgagc gaggggaacg ccaggttccg gatgaaccgc tcgttgaagc cgggagcccc
     1201 ggacagctcc aggtactcga tcagctcggg cagctcgagg gccatctccc gctcccggaa
     1261 cgacatgagg gccatcttcg cgccctcgct ggcggtgttg cccacggccc ggatccgttc
     1321 cacggggacc ggtgggacga gcccgatgat ccgggcgccg tcggggtcga tgtaggtccc
     1381 gaacgacccg gccagcagga cctcgtcgag gtctcggggg tcgatcccca gcgcgaccag
     1441 agcgacctca atccccgtgg cgatcgctcc cttggccgac tgcagctcgc ggatgtcgag
     1501 ctgggtgagc agcacctcgt cggtgagggc gaaggcgcgg atcccgtccc ggtccaccag
     1561 acgatcggcc aaggggtgct cggcgacggt ggcctcctcc caggacagca ggatgccgtt
     1621 ctccttcagc agtcccgaca cccgcagctg ggccacggcg tcgatcagcc cggagccgca
     1681 gatgccccgc ggctgcacgt ccccgccgat cacctggagc cggatcccgg aggggccgaa
     1741 ggtgaccccc tcgatcgctc cctcggtggc cctcatcccg tggaggatct ggccgccctc
     1801 gaaggcgggg cccgcgggcg ccgccgtggc gacgacccgg tccacggacc cgcacacgat
     1861 ctcgccgttc gttcccacgt ccaccagtag acgcatgcgg tcctcccgag cgagaccggt
     1921 ggccaccaca tcccccacga tgtccgctcc cacgtacgcg ccgatcgagg gcaggagcac
     1981 gacgcgcccc tcggggtgca gctggatgcc cacgtcccgc gcccggaggt cctgtgcctg
     2041 gaggaacgtg gccacgaacg gcgatagggc gaccgatcgg gggtccaccc cgagcaggag
     2101 atggagcatc gtggcgttgc cgaccaccac catctcgagg accgcttcca gttccacgcc
     2161 ggcggtggcg cacacgctct ccacgagccc gttcaccgtc gcgagcaccg cggctcgcag
     2221 gtcgtccagg gcctccgggc cggtcatcgc gtggcccatg cgggcgatga catcggcccc
     2281 gaacggcgcc tgacggttga tcgtggactc caccgccgtc gcgtttcccg aaaccaggtc
     2341 cacgagggtg gccaccacgg tagtggtccc gatgtcgagc gagatcccga accggcgatc
     2401 ggcggcgtcg cccggttcca cgtcgaccag ccggtcgccc accacggtgg cggtcacctc
     2461 ggagccctcg gagagggcgg cggccaggcg ggggagcagg gagggatccc aggagacctc
     2521 caacccctcg tcggcgagcg cctcacggat ccttcgcagg tggtcgcggg cgtcgtccaa
     2581 cgtgggagcc tccatcggga gcacgatccg tcgcacgttg ggttccagaa ggacgaaccg
     2641 tcctacgccc atggtggccg ccttgggggt ccgcatgggt cgcggcacct cgcagacggt
     2701 atcggcgcgg atctcagcct ggcaggccag acgccatccc gcctcgagct cagccgatga
     2761 caggtgccgc tggtcggccg tggtgggctc gccacgccct cccatcaccc gcacccggca
     2821 cttgccgcac gtcccccggc cgccgcaggt ggactcgatc ggcaggccgg cccagtgcgc
     2881 cgcgtggaac aggttcgtcc ccatcgggac gtgcaccgtc gcgccctgcg gcaggtacgt
     2941 gacctcgagc ttcctcatgc cgcggggccc tgcccggtcg aggccatcgc cgcgcgatgg
     3001 gccgcgatcc aggcggctcc gtgcgcatcg cggccgagca acagatccgc ggccaggaac
     3061 gccttgcgca cggggtcgtg gagcgggttc gtgatggcga cgttcatgcc ggtcaggatg
     3121 gccatggtca ggaacgctgc gtcgatgtgg tcgcggcccg gcatcccgaa ggagaggttc
     3181 gacgcgccgc acgagacgtt gacgccgagc tcgcgccgga tggcgcggat ggtctcgagc
     3241 atcgcggtgg cggcccgggg ctcggccccg atcggcatgg ccagggggtc gatgatcacg
     3301 tcttcgcgaa cgatcccgtg atcggcggcg gcctcgacga tggtccgcgc ggcggccagc
     3361 cggcgctcgg ggtccatgga gatgccggtc tcgtcgtggg ccatcccgat gaccgcggcg
     3421 ccgtggcgcc ccaccaccgg caggaggcgc gagagggagg cctcctcggc ggtgaccgag
     3481 ttcacgatga ccttgccccg ggaggcggcg atcgacgcct cgaggacctc gggggtggac
     3541 gagtcgatgc acagcggcac gttcaccacc tcctggaccg cgcgtacgac ctgagcgagc
     3601 atcgctgcct cgtcgaagcc ggggatcccg gcgttcacgt ccaggacgcg tgcgcctgcc
     3661 cggacctgtg cctcggcgtc ggcccgaacc gtgtccatca cgccggccac cagttcctcg
     3721 gtcagccgcg gccgcccggt ggggttgatc cgctcaccga tcaccacgaa cgggtggtcg
     3781 tccccgatgt acaccggccc gccgggcccc tcgaggatcg tggccacgcc catatcggcc
     3841 tagccgatca gccccttcgc cacctccacc gcttcggggg cgttgttcgc gtacccgtcg
     3901 gcgccgatct tctcggccca tccgggggtg accggtgcgc ccccgaccat caccttgacc
     3961 gccccgcgga gaccgtcgcg ttccagggcc ctgatcacgg tctcctggta gggcacggtg
     4021 gtcgtgagca gcgccgacaa tccgacgatc cgcggcccgt gctcgcggat cgcctctacg
     4081 atccggtcgg ccggcacgtc cttgcccaga tcgtgcacct cgaagcccga ggtccggagc
     4141 atggcggcca ccatgttctt gcccaggtcg tgcagatcgc ccttcaccgt ggccagcagc
     4201 accagcgatc gatcgttctc ctcgcgccgc tcgagcaagg cgggctccac gatctcgttg
     4261 cactgcttga agatctcggc cgaggccacc acctcgggga ggaagacctc accccgcttc
     4321 cacatctccc cgagctcgaa catggccacg gccatccccg cctcgaggat cgtccgcgga
     4381 tcaaggcccg accccgccag ccgccgggcc gtgtccaccg cggtggtgcg atcggccgag
     4441 atcaacgccc gtctcagggt ctggaggtcc tgctccatac ggccgctcct cgggccggag
     4501 tgggcggcga tcgtcgagtg accatcggtt agggttcgat cgccggttgg aacaggatga
     4561 taggaagcgc acgaggttcg tgggaatccc cctcccggta cacactgtga cccgaggtca
     4621 tgggctccgt tgccgccgcc cgggacgggg cgtagcgtcc ggagcggatc gcggccgtcg
     4681 cgaggagggg tcgtgcccga cgtgatcatc gtgggagccg gcatcaccgg cacgtcgatc
     4741 gccttccacc tggccacgcg gagctccctc ggcgtcctcc tggtggatcg ggcgggcgtg
     4801 gccgagggtg gcaccgggcg ctcctcggcg ctgatccgga tgcactacac gttccctccc
     4861 gaggtgcgct tggcgctgcg gagcctg
//
LOCUS       DASQQQ010000238         6001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_128968, whole genome shotgun sequence.
ACCESSION   DASQQQ010000238 DASQQQ010000000
VERSION     DASQQQ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128968"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /gene="mltG"
                     /locus_tag="VE646_09105"
     CDS             complement(<1..1005)
                     /gene="mltG"
                     /locus_tag="VE646_09105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HZD80183.1"
                     /translation="MTQPTSTAPPPPQEQPRLARRRHPPNWGLRLALAFLIVAGLVVA
                     AGVRYYSWCEDASGPQRRITFTVAEGASGGQIVDALHEHGVLRCGLVSKWLLRRSGKA
                     DAIRAGTYTLTTNMTPDDAFTALTKAPPAAPTVDLTIPEGYRLTQIAERVQQVLHVPA
                     QRFETNAGSGTWSLPPYLPKGKPLEGFLFPDTYRFLKKGTTPKAVIHTMLRNFRKRVS
                     DLPWSNAQKLGVTPYQVVTIASMIEKEARVEQDRPLIAAVIYNRLAKDMPLGIDATVA
                     YIDPDPSNGLTASDFLIDSPYNTRLHTGLPPTPIASPGLPSLQAALNPAQVDYLYYVL
                     C"
     gene            complement(1119..1574)
                     /gene="ruvX"
                     /locus_tag="VE646_09110"
     CDS             complement(1119..1574)
                     /gene="ruvX"
                     /locus_tag="VE646_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZD80184.1"
                     /translation="MDQTGEDPEGRATRVLALDLGDARIGVAISDPERRLAVPLGTVR
                     TGAPEDLKAVAGLVREHGVGLVVVGLPLTMAGERGVRAHHAERFAEALRAFLAVPVVL
                     HDERLSTVEAEGALRAAGIRGRERRRRVDRSAATVILQSYLDAERARGA"
     gene            complement(1549..4191)
                     /gene="alaS"
                     /locus_tag="VE646_09115"
     CDS             complement(1549..4191)
                     /gene="alaS"
                     /locus_tag="VE646_09115"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HZD80185.1"
                     /translation="MDGATIRETFLRFFEERGHKRVPSSPLIPPPESGLLLTNAGMNQ
                     FIPYFLGHAPAPFPRATSTQKCFRANDIENVGRTARHLTFFEMLGNFSFGDYFKRESC
                     GWGFELVTEVFGIDPDRLWVTVFRTDDEAAEIWLDIGIPAERIVRRGAEDNYWWTHAA
                     GPAGPCSEIYVDRGSRYGTEGGPEVDEERYLEIWNHVFMQEEVDERMQVVGDLPAKNI
                     DTGSSLERVAVVLQGVDSVFETDLFRPLLEVAEALAGIRHGDDPRADMSLKVVAEHGR
                     ATTFLIADGVQPSNEGRGYVLRRMLRRVVSHARRLGIETPVMERLVEVTVQRFGQAFP
                     ELVENQAYVAQVAASEEERFAGTLRQGMVVVEEEVQRTPAGGTVSGDVVFKLHDTFGF
                     PKELTRELVEDSGLSVDEGRFEELMTEQRERAKRAAKRARAGDELAGVSGPTEFVGYG
                     SLESDGRLLAVLADGVRAELAGEGQEVRLVLDRTPFYAESGGQIGDHGYLRTPGGLIR
                     VVDAQYGPGNVIVHSGVVLSGEVREGDEGHAEVDAMRRAATARAHTATHIVHWTLRHV
                     LGEHARQAGSLVAPGRLRFDFPHHAAVPRDVLEQAEEIANQRLSEDAPVTVYETTFEE
                     AKSQGAIALFGEKYGEFVRVVEVGDYSVELCGGTHVRRAGNVGVVRILGESSIGAGMR
                     RVEALVGPDALKEVNSERQLLQDVLDALGVGDRHTAPERVRELLEQNRRLSSELGKIR
                     KQDVAARAQEVAGRAVDVAGARLVVSAEVGSAEELRELAQAAVGRLEGSGGAAVVLGS
                     GAGGRALVVAACTKELVSRGVNAKQLLEPAARAVGGGAGGKPVLGTAGGPKGEALDEA
                     LGLIPARLEELAAGHEYGSDRRGP"
     gene            complement(4230..4451)
                     /locus_tag="VE646_09120"
     CDS             complement(4230..4451)
                     /locus_tag="VE646_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80186.1"
                     /translation="MRRLFWVALGLGAGATGALMTARFARRQMDKVAPATLARETRGG
                     LLELSKLVSESIAEGKRAMERREHELRER"
     gene            complement(4455..4904)
                     /locus_tag="VE646_09125"
     CDS             complement(4455..4904)
                     /locus_tag="VE646_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80187.1"
                     /translation="MAVIATAGDVALIVLAAFWGLLVLFLCVVLLNTFRMLESTKMLI
                     DGIREETMPLLAEVKTSVEKTNREIDRVDGMLVSAGAIVHRVERVTKLVEEAASSPLV
                     KMISFGAGVSKAVSRFRGDKGGRKKPPKTGSPAPSTSPPEPPQGEGD"
     gene            complement(5051..>6001)
                     /locus_tag="VE646_09130"
     CDS             complement(5051..>6001)
                     /locus_tag="VE646_09130"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZD80188.1"
                     /translation="LIDTQPTSTEPGAEPGRAHRWRGPAVAAVLPWLWFVVRLVGPEL
                     DLVAVGLPALAGLGILVYLAIAAARSRPGYVLSAASMVLFAIVSTLGPRLPHPTPAPV
                     DPFLLVSANTFDQNPTPGAAVRSLAALHPDILVAVETDEAIEHGLADAFGGYAHDMGG
                     RLEVYSRWPLRSGRRLPGIPKAVAFRVTVDRPGGSVIVVAIHLPNPLHEISFPDHLAM
                     VRRILAEVRDLPHPVILAGDFNTSDRTDAYRVMAGDMRDAIRSSWPSRTYVGGLFGLL
                     ELRIDHVLEPEDWCSQDAVTVPVEGSDHRGLVVRLGPCPP"
BASE COUNT          845 a         2200 c         2115 g          841 t
ORIGIN      
        1 gcagagcacg tagtagaggt agtcgacctg ggcggggttc agggccgcct gcagggacgg
       61 cagtccagga ctcgcgatgg gcgtgggggg caggcccgtg tgcagccgcg tgttgtacgg
      121 gctgtcgatc aggaagtccg aggcggtgag accgttggag gggtcgggat cgatgtaggc
      181 gaccgtggcg tcgatcccca ggggcatgtc cttggccagg cggttgtaga tcaccgccgc
      241 gatcagcggc cgatcctgct cgacccgggc ctccttctcg atcatcgagg cgatggtcac
      301 cacctggtag ggggtgacgc cgagcttctg ggcgttggac cagggcaggt ccgagacgcg
      361 cttgcggaag ttccggagca tcgtgtggat cacggccttg ggggtggtgc ccttcttcag
      421 gaagcggtag gtgtcgggga acaggaagcc ctcgagcggc ttgcccttcg gcaggtacgg
      481 gggcagggac caggtgcccg agccggcgtt cgtctcgaac cgctgggccg gcacgtgcag
      541 gacctgctgg acccgctcgg cgatctgggt gagtcggtag ccctccggga tggtgaggtc
      601 gaccgtcggt gcggccggcg gggccttcgt gagcgcagtg aaggcgtcgt cgggggtcat
      661 gttcgtggtg agggtgtacg tgccggcccg gatcgcgtcc gccttgccgc tccggcggag
      721 cagccatttg gagaccagtc cgcaccgaag gaccccgtgc tcgtgcaagg cgtcgacgat
      781 ctggccgccg gaggccccct cggcgaccgt gaacgtgatc cgacgctggg gaccggaggc
      841 gtcctcgcac cagctgtagt agcggacgcc ggcggcgacc accaagccgg ccacgatgag
      901 gaaggcgagg gccagtcgaa gcccccagtt gggggggtga cgacggcgtg cgaggcgcgg
      961 ctgctcctgc ggaggcgggg gcgcggtcga cgtgggctgg gtcacggttt tcgaggatac
     1021 ccggcacggg aaggcggcca tgcccaggcg cttgacgcga cccggggatc aacggtgagg
     1081 ggggcgcgga cggtgaccgc tgggggcctc gagagaggtc acgccccgcg ggcgcgttcg
     1141 gcgtccagat agctctgcag gatcaccgtg gccgccgagc ggtccacccg acgccggcgc
     1201 tcgcggcccc ggatgccggc cgcgcgcagc gccccctcgg cctccaccgt ggacaatcgc
     1261 tcgtcgtgca gcaccacggg tacagccagg aacgctcgga gcgcctcggc gaagcgctcg
     1321 gcgtggtgcg ctcgcacacc gcgctccccc gccatcgtga gggggagccc caccaccacc
     1381 agacccaccc cgtgctcccg caccagcccg gccacggctt tcaggtcctc tggcgccccg
     1441 gtccgcaccg tccccagggg caccgccaga cgccgctcgg ggtccgagat ggccacgccg
     1501 atccgcgcgt cgccgaggtc cagggcaagg acccgggtcg cgcgcccttc agggtcctcg
     1561 ccggtctgat ccatactcgt ggcccgcggc gagctcctcc agccgggccg ggatcagccc
     1621 cagcgcctcg tccagggcct cgcccttcgg cccgcccgcc gtgcccagga ccggcttgcc
     1681 ccccgctcct ccaccgaccg ctcgagccgc cggctcgagc agctgcttgg cgttgacccc
     1741 ccggctcacg agctccttcg tgcaggccgc caccaccagg gcccgtccgc cggccccgct
     1801 gccgagcacc accgctgccc ctcccgaccc ctccaaccgg cccaccgctg cctgcgccag
     1861 ctcccggagc tcctccgccg agccgacctc cgccgagacc accagtcgag cccccgccac
     1921 gtccaccgcg cggccggcca cctcctgggc ccgggccgcc acgtcctgct tgcggatctt
     1981 gccgagctcg ctggacagcc gccggttctg ctcgagcagt tcccggacgc gctcaggcgc
     2041 cgtgtgccgg tcgccgaccc ccagcgcgtc gagcacgtcc tgcaggagct ggcgctcgga
     2101 gttgacctcc ttgagcgcgt cgggacccac cagcgcctcc acccggcgca tcccggcccc
     2161 gatgctggac tcgcccagga tccggaccac gcccacgttg ccggcccgcc gcacgtgcgt
     2221 gccgccgcag agctcgaccg agtaatcgcc cacctcgacc acgcgcacga actcgccata
     2281 cttctcgccg aacagcgcga tcgcgccctg gctcttcgcc tcctcgaagg tggtctcgta
     2341 gaccgtgacc ggcgcgtcct cggacaggcg ctggttggcg atctcctcgg cctgctcgag
     2401 gacgtcgcgc ggcaccgccg cgtggtgggg gaagtcgaac cgcagccggc cgggcgccac
     2461 cagcgagccc gcctgacggg cgtgctcgcc cagcacgtga cggagggtcc agtgcacgat
     2521 gtgcgtggcg gtgtgcgctc gggccgtcgc cgcccggcgc atcgcgtcca cctcggcgtg
     2581 gccctcatcg ccctctcgga cctcgcccga cagcaccacg cccgagtgca cgatcacgtt
     2641 ccccggcccg tactgtgcgt ccaccaccct gatcagcccg cctggcgtgc gcaggtaccc
     2701 gtggtcaccg atctgaccgc ccgactcggc gtagaagggc gttcggtcca ggaccagccg
     2761 cacctcctga ccctcccccg ccagttcggc ccggacgccg tcggccagca ccgccagcag
     2821 gcgcccgtcc gactccagcg agccgtagcc cacgaactcg gtggggcccg agaccccggc
     2881 cagctcgtcg ccggccctcg cgcgcttggc ggcacgcttg gcccgctcgc gctgctcggt
     2941 catcagctcc tcgaaccggc cctcgtcgac cgacagcccg gagtcctcga ccagctcgcg
     3001 cgtgagctcc ttggggaagc cgaacgtgtc gtgcagcttg aagaccacgt cgcccgagac
     3061 ggtcccgccg gccggcgtgc gctgcacctc ctcctcgacc accaccatcc cctggcggag
     3121 ggtgccggcg aagcgctcct cctcagaggc ggcgacctgg gccacgtagg cttggttctc
     3181 gacgagctcg gggaaggcct gtccgaaccg ctgcacggtg acctcgacca gccgctccat
     3241 cacgggcgtc tcgatcccca gccgacgagc gtggcttacc acccggcgga gcatccgccg
     3301 gagcacgtag ccccgaccct cgttcgaggg ctgcacaccg tccgcgatca ggaacgtggt
     3361 ggcccggcca tgctcggcca cgaccttcag ggacatgtcc gcccgagggt cgtcgccgtg
     3421 ccggatcccg gccagcgcct cggcgacctc cagcagcggg cggaacaggt ccgtctcgaa
     3481 gacgctgtcc accccctgta ggaccacggc cacccgctcc aggctcgagc ccgtgtcgat
     3541 gttcttggcc ggcaggtcgc ccacgacctg catccgctcg tcgacctcct cctgcatgaa
     3601 cacgtgattc cagatctcca ggtaccgctc ttcgtccacc tcgggtccgc cctcggtccc
     3661 gtacctcgag ccgcggtcca cgtagatctc ggagcacggg ccggccgggc cggcggcgtg
     3721 agtccaccag tagttgtcct ccgccccgcg ccggacgatc cgctccgcgg ggatcccgat
     3781 gtccagccag atctcggcgg cctcgtcgtc ggtacggaag acggtgaccc acaggcggtc
     3841 cggatcgatg ccgaagacct cggtcaccag ctcgaacccc cagccgcagg actctcgctt
     3901 gaagtagtcg ccgaaggaga agttgcccag catctcgaag aacgtgaggt gccgcgcggt
     3961 gcggccgacg ttctcgatgt cgttggcccg gaagcacttc tgggtggagg tggcgcgggg
     4021 gaagggcgcc ggggcgtgtc ccaggaagta cgggatgaac tggttcatgc ccgcgttcgt
     4081 gagcagcagg ccggactcgg gcggggggat caggggcgaa gagggaacgc gcttgtgccc
     4141 gcgctcctcg aagaagcgca ggaacgtctc acggatcgtc gcaccgtcca tcgggaacgg
     4201 tcagtctacc ggggcaacgg gggccgccct cagcgctcgc ggagctcgtg ctcccgccgt
     4261 tccatggcgc gcttgccctc ggcgatggac tcggagacga gcttggagag ctcgagcagc
     4321 ccgccccgcg tctcgcgggc cagggtggcg ggtgccacct tgtccatctg ccggcgggcg
     4381 aaccgggccg tcatcagggc gccggtggcc cccgcgccga gaccgagggc gacccagaac
     4441 agccgtcgca ccgctcagtc gccctccccc tgggggggtt cgggagggga ggtggacggc
     4501 gcggggctcc cggtcttcgg gggcttcttg cgacccccct tgtcaccccg gaacctcgac
     4561 acggccttgg agaccccggc cccgaaggag atcatcttga ccagtggcga gctggccgcc
     4621 tcctccacga gcttcgtgac ccgttccacc cggtgcacga tcgcgccggc agagaccagc
     4681 atgccgtcca cccggtcgat ctcgcggttg gtcttctcca cggaggtctt gacctcggcc
     4741 agtagcggca tggtctcctc acggatgccg tcgatcagca tcttcgtgct ctcgagcatc
     4801 cggaacgtgt tcagcagcac cacgcacagg aacagcacca gcagtcccca gaacgcggcc
     4861 aggacgatca gggcgacgtc cccggcggtg gcgatcacgg ccatcaggca actccttggg
     4921 tggctcggtc cgccgcatca taccgggcag acccggagcg ccggccgtcg cgcggtcggc
     4981 tcgtgggggt ggctcctgcg cgctcggggg cgggttcggg gacggcgggc gtcccgctcg
     5041 gggccggccg tcagggggga cagggcccca ggcggaccac gagcccgcgg tggtccgagc
     5101 cttccaccgg aaccgtgacg gcgtcctgcg agcaccagtc ttcaggctcg agcacgtggt
     5161 cgatccgcag ctcgagcagg ccgaagaggc cgccgacgta ggtccggctc ggccagctcg
     5221 agcggatcgc gtcgcgcatg tcgccggcca tgacccggta ggcgtcggtg cgatcgctgg
     5281 tgttgaagtc gccggccagg atcaccgggt ggggcaggtc gcggacctcg gccaggatcc
     5341 ggcggaccat cgccagatgg tccgggaacg agatctcgtg cagcgggttc ggtaggtgga
     5401 tcgccaccac gatgacgctg ccccccggcc ggtcgaccgt gacccggaag gccacggcct
     5461 tggggatgcc gggcaggcgc cgacccgacc ggagcggcca ccgcgagtag acctccaggc
     5521 ggccccccat gtcgtgcgcg tagcccccga aggcgtcggc cagtccgtgc tcgatcgcct
     5581 cgtcggtctc cacggccacg aggatgtcgg gatgcagggc cgccagcgag cggacggcgg
     5641 ctccgggcgt gggattctgg tcgaacgtgt tggccgagac caggaggaac gggtccaccg
     5701 gcgctggtgt ggggtgcggg agccgcggtc cgagcgtgga gacgatggcg aagagcacca
     5761 tcgacgcggc cgagagcacg tagccgggac gggagcgcgc cgcggcgatg gccaggtaga
     5821 cgaggatgcc gagcccggcg agcgccggga ggcccacggc caccagatcc agctcgggcc
     5881 ccaccaggcg gaccacgaac cacagccacg ggaggacggc ggcgacggcc ggccctcgcc
     5941 atcgatgggc ccggcccggc tcggcaccgg gctcggtgct ggtgggttgc gtgtcgatca
     6001 g
//
LOCUS       DASQQQ010000239         8028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_44026, whole genome shotgun sequence.
ACCESSION   DASQQQ010000239 DASQQQ010000000
VERSION     DASQQQ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8028
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44026"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1591
                     /locus_tag="VE646_09135"
     CDS             <1..1591
                     /locus_tag="VE646_09135"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HZD80189.1"
                     /translation="ARPVTDLLIHDARILDGTGRPAFPGAVAVRSDRIERLILPGEPE
                     PPAHRRLDARGLLVTPGFVDVHDHSDLSPFVEPWMDSMLRQGVTTVVVGNCGSSAVPF
                     AGADELAAWTGRSPEDLGQRWTSFSGYREAVQAARPAVNLAALVGHGSLRLEVMGDVS
                     RIPTTDEMARMRGLLADAVEEGAVGLSTGLIYRPGIHATTQEIQELAAVLRDTGALYA
                     SHIRGEGQPVFEAIGECIGIGRTAGVPAHVSHLKLETSLVWGRTADLLGTIDRARAAG
                     DDVSADQYPYAAWESALSSLLPPWAPPDALPDLLGDPRTAARLIRSVEQGEPGWQSSV
                     RGVGWERLVVVAHAGAPGLTGRTLADIGAATGRSPAEATFDLLMIDPETSVIGHAMHE
                     DDVRAIVAREDVMVATDGVAVSPTGPMGRFHLHPRCYGTFPRVLARYVRREGVLSLTA
                     AVRKMTSLPAERFGLRDRGVVRTGAFADLVILDPATVEDRATYEAPHAFPDGIETVIV
                     NGRIAWKGRRVERAGRALRRG"
     gene            complement(1620..2585)
                     /locus_tag="VE646_09140"
     CDS             complement(1620..2585)
                     /locus_tag="VE646_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005480028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD80190.1"
                     /translation="MIRYIIRRVIWVLLVMLIITVLTYLIFFVMPPTDPAVAFAGKAA
                     TPQMIAEVRHLFGLDKPLWEQYLLFVKHLFLGDRWGWPGLGFSFTTRSSVRSIIASRI
                     IVTTQLAVGAALIWLAIGIPIGVLSALRPRTLTDRVAMGFALFGVSAPVFWLGLMALW
                     LFWFKLGIAAGSGYVSIGTSFFGWLNHMLMPWFVLAFLFAAFYARITRAGLIETMNED
                     FVRTARAKGVPEWKVVLKHGLRASLTPVVTMLGMDLGTLLGGAIITETVFNLQGIGLY
                     AVQSVYNGDLPAILAVTVIASLAISVMNLIVDIVYAFLDPRIRYT"
     gene            complement(2667..4523)
                     /locus_tag="VE646_09145"
     CDS             complement(2667..4523)
                     /locus_tag="VE646_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZD80191.1"
                     /translation="MKGKRLAAVGMIAAIAMVGAACSSAKAGSSPSSTATPAPGGVYR
                     TAISSFGFVGGFDPTGEYLGLAFDFYGAMLRNLVTYDHVAGAAGNTLVPDLATSVPQP
                     TDGGLTYTFHLKPGIMFGPPVDRPITSQDVAYAFERIDSRPEVAQYAFYYDGTIVGMY
                     QHSGPPTPISGIQTPNASTIVFHLTRPTGDFLYRLAMPAAAPIPREVAKCFPTAGGYG
                     RDVVSSGPYMIQGADKVDISSCSSIKPMSGFDPSSHLTLVRNPQYDPSTDSTSDRSNY
                     VNGISVVVDSNVSDIFNKVRTGALDGSLADQPPATVLQQYESSSSLKPMLHLNSGDRT
                     WYITMNLTTPPFDDVHVRKAVNDIIDKAALQQAWGGPVEGQIATHIMPPTVLDNQLTN
                     AYDPYATPNEAGSLSMAKQQMMQSKYDPKHDGMCDVSACKNIIMINRNIAPWTNMEPT
                     VVQDLAKIGIQVVPRELETSAAYKDIETVKDDVPIALNAGWGKDYPDPYTFAEALFGS
                     GSINPTGNPNYSLVGLTAPQATSLGIAYPAGGVPSVDGRINRCEALSGSSRLSCWIGF
                     DKYMMTSVVPWVPYLWANQVTITAPSVTRYQFDQFSGYISLTQIAVNNHASV"
     gene            complement(4549..5076)
                     /locus_tag="VE646_09150"
     CDS             complement(4549..5076)
                     /locus_tag="VE646_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD80192.1"
                     /translation="MQPGLRLVVAIIALFTWPYIGRIVRGQTLSVRQRDFVEAARSLG
                     LGNLRIMVREILPNIIAPVVVYTTLIIPSNILFEAALSFLGVGIPQSTPSWGGMLAQA
                     AENQLFTYAWWMMLFPGLFLLLATIAFNLVGDGLRDALDPRPLLVRRKDKRKRRRERA
                     AAGAVARDEELPVGT"
     gene            complement(5095..5595)
                     /locus_tag="VE646_09155"
     CDS             complement(5095..5595)
                     /locus_tag="VE646_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80193.1"
                     /translation="MDAALGGAGGEGGTNVVSRSLWRAFWRRFKADRLAMAGLATILL
                     LILLAILAPSIAGLIGHGPNQLFGGMVTPFGLPKGPSAHFWFGADPVGRDVFVRTIYG
                     ARTSLTVAFLATGIATLIGVILGLTAGYLGGWVDMVVSRAIDVVLSLPLLLFAIGISA
                     ACSVTV"
     gene            complement(5739..6050)
                     /locus_tag="VE646_09160"
     CDS             complement(5739..6050)
                     /locus_tag="VE646_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80194.1"
                     /translation="MLQVTDTAVGVFRRILEMPDVDGSAIRIQVGELPTGEPGIMFRP
                     VEGPETGDLESESSGMDVFVSDELAQPLDGSVLDARETPEGATLYLRPQGDDRAEPPP
                     A"
     gene            6332..7114
                     /locus_tag="VE646_09165"
     CDS             6332..7114
                     /locus_tag="VE646_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZD80195.1"
                     /translation="MPSGSIVVAAAGDLCLPEPASCAPTADLAAAIQPDLVLVLGDNQ
                     YEDGTLRDYRSSYGSTWGRFRSITRPVAGNHEWHTTDAQGYLDYFGTNRYWYSYDRAG
                     WHFVALDGTCDEDGGCGPGSPQYEWLKADLAGAHPCILAYWHQPRWSSGTRHGSDASV
                     GPFWDLLAAAGADLVLNGHEHNYERFAPQDPSGVADPSGIVEIVAGTGGKGQYPFGIP
                     IANSLARSEGPGIVELVLGPDHWRERFILTDGTIADTASGSC"
     gene            7195..7365
                     /locus_tag="VE646_09170"
     CDS             7195..7365
                     /locus_tag="VE646_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80196.1"
                     /translation="MEAKVVRASEMERLGIRVTQEGGGVRYLEGAKHGLSTSIYEGEV
                     LPGSGPGAHAHP"
     gene            7366..7569
                     /locus_tag="VE646_09175"
     CDS             7366..7569
                     /locus_tag="VE646_09175"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZD80197.1"
                     /translation="MEVAVVCSGRVTYTIGERSFEAGPGDVVVIPAGALHDFSNPGDT
                     AVRLIAIHHAPVHAGTHLPAMGR"
     gene            complement(7870..7943)
                     /locus_tag="VE646_09180"
     tRNA            complement(7870..7943)
                     /locus_tag="VE646_09180"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7907..7909),aa:Thr,seq:tgt)
BASE COUNT         1298 a         2677 c         2803 g         1250 t
ORIGIN      
        1 ggcccggccc gtgaccgacc tcctgatcca cgacgctcgg atcctcgatg gcaccggccg
       61 ccctgccttc ccgggcgcgg tggcggtacg gagcgaccgc atcgagcggc tgatcctgcc
      121 gggcgaaccc gaaccgccgg cccaccgacg gctcgacgct cggggactcc tcgtgacgcc
      181 cgggttcgtg gacgtccacg atcactcgga cctatcgccg ttcgtcgaac cctggatgga
      241 cagcatgctc cggcagggcg tgaccaccgt ggtggtgggc aactgcggat cgtcggccgt
      301 ccccttcgcg ggggctgacg agctggccgc atggaccggg aggtcccccg aggacctcgg
      361 gcagcgctgg accagcttct ccgggtaccg ggaggcggtg caggcagccc gaccggcggt
      421 caacctggcg gcgctcgtgg gacatgggag cctgcggctg gaggtgatgg gggatgtctc
      481 ccgcatcccc accacggatg agatggcccg catgcgaggg ctgcttgccg acgcggtgga
      541 ggagggcgcg gtcggactgt cgaccgggct gatctaccgc cccggcatcc atgccaccac
      601 ccaggagatc caggagctgg cggcggtgct ccgcgacacc ggcgccctgt acgccagcca
      661 catccgcggc gaggggcagc cggtgttcga ggcgatcggc gagtgcatcg gcatcggccg
      721 aacggccggg gtgcccgccc acgtcagcca cctgaagctc gagacctcgc tggtctgggg
      781 tcggacggcg gatctgctcg ggacgatcga ccgggcccga gcggccggag acgacgtcag
      841 cgcggaccag tacccctacg ccgcctggga gagtgccctg tcctccctgc tcccgccgtg
      901 ggcaccgccc gacgccctgc cggacctcct gggcgacccc cggacggcgg cccggctgat
      961 ccgcagcgtg gagcaagggg aacccggatg gcagagcagc gtgcgggggg tgggctggga
     1021 gcgactggtc gtcgtggccc acgccggagc tcccggactg accggacgca cccttgccga
     1081 catcggcgca gccacgggtc gctcacccgc cgaggcgacc ttcgatctgc tgatgatcga
     1141 ccccgagacc agcgtgatcg ggcacgccat gcacgaggac gacgtccgcg ccatcgtggc
     1201 gagggaggac gtgatggtgg ccaccgacgg cgtcgcggtc tcgcccaccg gacctatggg
     1261 gcgtttccac ctgcaccccc gctgctacgg gacgttcccc cgggtcctgg cccggtacgt
     1321 gcggcgggag ggggtcctgt ccctcaccgc cgcggttcgg aagatgacct cgctccctgc
     1381 cgagcggttc gggctgcggg accgcggggt ggtccggacg ggcgcgttcg ccgacctggt
     1441 gatcctggac cctgccaccg tggaggaccg cgcgacgtac gaggcgccgc acgcgttccc
     1501 ggatggcatc gagacggtga tcgtgaacgg tcggatcgcg tggaagggcc gtcgggtcga
     1561 gcgcgccggc cgagcgctcc gccgaggctg atcgcccctc ctcggctccg gcccggggtt
     1621 caggtgtacc ggatccgcgg gtccaggaag gcgtagacga tgtccacgat caggttcatc
     1681 acgctgatgg ccagcgaggc gatcaccgtc accgccagga tcgcgggtag gtcgccgttg
     1741 tagacggact ggacggcgta gagcccgata ccctgcaggt tgaagacggt ctcggtgatg
     1801 atggccccac ccagcagggt ccccaggtcc atccccagca tggtcaccac cggggtgaga
     1861 ctggcccgga ggccgtgctt cagaacgacc ttccactccg ggacgccctt cgcccgggcc
     1921 gtgcggacga agtcctcgtt catcgtctcg atcagtccgg cgcgggtgat cctcgcgtag
     1981 aaggcggcga acaggaaggc cagaacgaac cacggcatga gcatgtggtt cagccagccg
     2041 aagaagctgg tgccgatcga gacgtacccg ctgccggcgg cgatgcccag cttgaaccag
     2101 aacagccaga gggccatgag tcccagccag aacaccggag ctgatacgcc gaagagggcg
     2161 aaccccatgg ccacccggtc cgtcagggtt cggggccgga gtgccgagag cacgccaatc
     2221 gggatcccga tcgccaacca aatgagcgcg gcgcccacgg ccagctgcgt ggtgacgatg
     2281 atgcggctgg cgatgatcga ccgtacggag gaccgcgtgg tgaaggagaa ccccagaccc
     2341 ggccaccccc accggtcgcc caggaacagg tgcttcacga acaggaggta ctgctcccac
     2401 aagggcttgt cgagcccgaa gaggtgacgc acctcggcga tcatctgcgg ggtggccgcc
     2461 ttgccggcga aggcgaccgc ggggtccgtc ggcggcatga cgaagaagat caggtaggtg
     2521 aggacggtga tgatgagcat caccagcaag acccagatca cgcgacggat gatgtaccgg
     2581 atcacgtcga tggctcctcg gcgccgggcg gcgcgggtcc aggcctccgg ggagaccgga
     2641 cccgcgccgc cttcgttcct atcgcctcac actgaggcgt ggttgttcac ggcgatctgc
     2701 gtgagcgaga tgtagccgga gaactggtcg aactggtacc tcgtgacgct gggcgccgtg
     2761 atcgtgacct ggttggccca caggtacggg acccagggga ccacgctggt catcatgtat
     2821 ttgtcgaagc cgatccagca actcagccgg gaggagccgg acagcgcctc gcaccggttg
     2881 atccgtccgt ccacgctggg gaccccaccg gccgggtagg cgatcccgag gctcgtcgcc
     2941 tgcggggccg tgaggccaac cagcgagtag ttcgggttgc ccgtggggtt gatggaccca
     3001 ctgccgaaca gggcttcggc gaaggtgtaa ggatcggggt agtccttgcc ccacccggcg
     3061 ttgagggcaa tcggcacgtc gtccttgacg gtctcgatgt ccttgtaggc cgcgctggtc
     3121 tcgagctccc ggggaaccac ctggatgccg atcttggcca gatcctgcac caccgtgggt
     3181 tccatgttgg tccagggggc gatgttgcgg ttgatcatga tgatgttctt gcaggcggag
     3241 acgtcgcaca tcccgtcgtg cttcgggtcg tacttcgact gcatcatctg ctgcttggcc
     3301 atcgacaacg atccggcctc gttcggcgtc gcgtacgggt cgtaggcgtt cgtcaactgg
     3361 ttgtccagca cggtgggcgg catgatgtgg gtggcgatct gcccctccac gggaccgccc
     3421 cacgcctgct gcaacgccgc cttgtcgatg atgtcgttca ccgccttgcg gacgtggaca
     3481 tcgtcgaacg gcggcgtggt caggttcatg gtgatgtacc aggtccgatc gcccgagttc
     3541 aggtggagca tcggcttcag cgacgagctg gactcgtact gctgcaggac cgttgccggg
     3601 ggctgatcgg ccagcgaacc atccagggcc ccggtgcgga ccttgttgaa gatgtcgctg
     3661 acgttggaat ccaccaccac ggagatgccg ttcacgtagt tggagcgatc gctcgtgctg
     3721 tccgtggagg ggtcgtactg ggggttgcgg accagcgtca ggtggctgga gggatcgaag
     3781 cccgacatgg gcttgatcga gctgcacgag gagatgtcca ccttgtcggc cccctggatc
     3841 atgtagggac ccgacgagac cacgtctctt ccgtagccgc ctgccgtggg gaagcacttg
     3901 gcgacctccc gcgggatcgg ggcggcggcg ggcatggcca ggcggtacag gaagtcgccc
     3961 gtcggcctgg tcaggtggaa cacgatggtg ctggcgttgg gcgtctggat ccccgagatg
     4021 ggcgtcggtg gcccggagtg ctggtacatg cccacgatcg tgccgtcgta gtagaacgcg
     4081 tactgggcca cttcgggacg ggaatcgatc cgctcgaaag cgtaggcgac gtcctgcgac
     4141 gtgatggggc gatccaccgg gggcccgaac atgatccccg gcttgagatg gaacgtgtag
     4201 gtgagcccgc cgtcggtcgg ctgaggcacc gaggtggcca ggtctggcac caacgtgttc
     4261 ccggcggcac cggcgacgtg atcgtaggtc accaggttgc gtagcatggc gccgtagaag
     4321 tcgaaggcca gacccaggta ctcgcccgta gggtcgaatc cgccgacgaa gccgaagctc
     4381 gagatggccg tccggtagac gccaccggga gcgggcgtcg cggtggagct ggggctgctt
     4441 ccagccttgg cgctggagca tgcagccccg accatcgcta tggccgcgat catcccgacc
     4501 gcagccaacc tcttcccttt catgagggtt ccttcctcca cttccctttc acgtgccaac
     4561 ggggagctcc tcgtccctgg ccaccgcgcc cgcagcggcc cgttccctcc gacgcttgcg
     4621 cttgtccttc ctccggacca acaagggcct ggggtcgagg gcgtcgcgca gcccgtcgcc
     4681 caccaggttg aaggcgatcg tcgccaggag caggaacagg ccggggaaca gcatcatcca
     4741 ccaggcgtag gtgaacagct ggttctcagc ggcctgcgcc agcatgccgc cccaggacgg
     4801 cgtgctctgc gggatcccca ctcccaggaa cgacagggcg gcttcgaaca ggatgttgct
     4861 cgggatgatc agcgtcgtgt agacgaccac cggcgcgatg atgttgggga ggatctcgcg
     4921 aaccatgatc ctcaggttcc ccaagcccag ggaccgggcc gcctcgacga aatcgcgctg
     4981 tcggaccgag agggtctgcc cgcgaacgat ccggccgatg tagggccacg tgaacaaggc
     5041 gatgatggcc accaccaacc tgaggccggg ctgcacgagc cctcccaggc agcctcatac
     5101 cgtcaccgag caggcggccg agatgccgat cgcgaacagc agcaggggca gggataggac
     5161 cacgtcgatg gccctggaga ccaccatgtc cacccacccg ccgaggtagc cggcggtgag
     5221 ccccaggatc acgccgatga gcgtggcgat gccggtggcc aggaaggcca cggtgagcga
     5281 ggttcgcgcc ccgtagatcg tccgcacgaa cacgtcacgt ccgaccgggt cggccccgaa
     5341 ccagaagtga gcgctcggtc ccttggggag accgaacggt gtgaccatgc cgccgaacag
     5401 ctggttcgga ccatgcccga tcaggccggc gatcgatgga gcaaggatgg cgagcaggat
     5461 gaggagcagg atcgtggcca gcccggccat ggccagccga tcggccttga accgacgcca
     5521 gaacgcgcgc cagagagagc ggctgacgac attagtgcct ccctctccgc cggctccacc
     5581 gagtgcagca tccatgggac ctcggttccg gggcgtcgtg tctggcggtt cctgcgcgga
     5641 tcccgtgtcg atccgccgaa aggtattcgc ccggtctggt cacgaagttc gccccgaggc
     5701 cccatggtgc gtggcggagc tcagctgtag gggggagctc aggcaggagg cggctctgcg
     5761 cggtcatcgc cctgcggacg caggtagagc gtggctccct cgggggtctc ccgagcgtcg
     5821 agcactgacc catccagggg ctgtgccagt tcatcgctca cgaacacatc catcccggac
     5881 gactcgctct ccagatcccc ggtctcgggt ccttccacag gacggaacat gatcccgggt
     5941 tcaccggtgg gcagctctcc cacctggatg cggatggcgc tgccatccac gtcgggcatc
     6001 tcgaggatcc tgcggaacac gcccacggcg gtgtcggtca cctggagcat ggggggcctc
     6061 ccttcggtcg aggggtcggt ccctcccacc ctacccgaga tggctcggcg tcacccctgg
     6121 ggccggttgc gatggcgcgc cgcctccgca taggttgggg tcggtggagg cccgccgtcg
     6181 tcccccggcg ctcgtcctgg tgctcgcgct cctggccgcg gcatgtgcgt ccgcctcggg
     6241 ccgctccgga tcatccccac cggctaccgc cgccggctct gccgcctcgc cggctccgac
     6301 cgcgtccacc ccgtcggtcc aggcggagca cgtgccgagc ggatccatcg tcgtggccgc
     6361 agccggggac ctgtgcttgc ccgagcccgc gtcctgtgcg ccgacggccg acctggctgc
     6421 ggcgatccag ccggacctcg tcctcgtcct gggggacaac cagtacgagg acgggaccct
     6481 gcgcgactac cgatccagct acggctccac ctgggggcgg ttccgatcga tcacccggcc
     6541 cgtggccggc aaccacgagt ggcacacgac cgacgcccag ggctacctcg actacttcgg
     6601 gacgaaccgg tactggtact cgtacgaccg agccggatgg cacttcgtcg cgctcgacgg
     6661 gacctgcgat gaggatggcg ggtgtggtcc cggctcgccc cagtatgagt ggctgaaggc
     6721 cgacttggcg ggagcgcacc cctgcatcct ggcctactgg caccagcccc gttggagctc
     6781 tgggacccgc cacggctccg acgcatcggt cggccccttc tgggacctgc tcgccgcggc
     6841 cggtgccgac ctggtgctca acggccacga gcacaactac gagcgcttcg caccacaaga
     6901 tccgtccggg gtcgcggacc cttcggggat cgtggagatc gtggcgggaa cgggtggcaa
     6961 gggtcagtac ccgttcggca taccgatcgc gaactccctg gcgaggtccg agggcccggg
     7021 gatcgtggag ctcgtcctgg ggcccgatca ctggcgggag cggttcatcc tcaccgacgg
     7081 gacgatcgcc gacacggcct cgggctcctg ctgaggaggg atccctctcg gaccaccatc
     7141 catgagaagc tgccgctcca cctgatcgag atcaccggac cgggaggatt cctgatggaa
     7201 gccaaggtcg tgcgggcgtc ggagatggaa cggctcggga tccgcgtcac ccaggagggg
     7261 ggtggtgttc ggtacctgga aggtgccaag cacggcctgt ccacctcgat ctacgagggg
     7321 gaggtgctcc ccggcagcgg acccggagcg catgcccacc cctaggtgga ggtcgcggtc
     7381 gtctgctcgg gtcgggtgac ctacacgatc ggggagaggt cgttcgaggc cggccccggc
     7441 gacgtggtgg tcatccctgc cggggcctta cacgacttct ccaaccccgg cgacaccgcc
     7501 gtgaggctga tcgcgatcca ccacgcaccc gtgcacgcgg gcacgcacct acccgcgatg
     7561 gggcgctgat ccgatgtgcg taccgtggcg ggtgccgaag ccggaaacgc gagcgcgtga
     7621 tcggacccga aactgccccc acgtccatct gggacgttga cgctcgtatc tccctatcga
     7681 cccaggcacc atcttgtccg gatgcggccg aaagcccgtg ggccacgcgt cgttcggagc
     7741 ggtcggacct accgccctgt ggcggttcgc ctgcagaggt ccgacttcac ggacccctca
     7801 tgggtagcgg ccatcgcggt cggatcgagc ggagcggagg agaaggcggt ggccacctgg
     7861 tagagcgaga gccggcggtc ggattcgaac cgacgacctg ctgtttacaa gacagctgct
     7921 ctggccatct gagctacgcc ggcgacggag cgagtctacc ggtcaaggcg agcgggacgg
     7981 cagcacgtaa ccatacagta ccgccgccag tgacgcagag gcgttcaa
//
LOCUS       DASQQQ010000240         7590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_265062, whole genome shotgun sequence.
ACCESSION   DASQQQ010000240 DASQQQ010000000
VERSION     DASQQQ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7590
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265062"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VE646_09185"
     CDS             complement(<1..732)
                     /locus_tag="VE646_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/calcium exchanger protein"
                     /protein_id="HZD80198.1"
                     /translation="MTELRDELREVHPTRQRVTIALALGATLPGLALRFWGLHPADAI
                     QAVLFGLAIVGAAFLLSWAAEVAQLDISAGLAIAVLALIAVLPEYAVDMVFALKGGHA
                     VQAYGPACRAPGAGGDTACSLALANMTGANRLLIGIGWSMVVFIAWYRWRSRGRPHEE
                     VVLTRQHSVEVTFLAIATVYSLTLPLKHSVTLWDSLVLVAIFVAYTIRISRAPAEEPH
                     LVGPARYLGSLPVAHRRVAVVVMFVF"
     gene            963..1997
                     /locus_tag="VE646_09190"
     CDS             963..1997
                     /locus_tag="VE646_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HZD80199.1"
                     /translation="MEWPELIASLFVILGGAELFTNGVEWIGEGFGLSEGAVGSVLAA
                     VGTALPETVLPLIAILSGHASGEQIGIGAILGAPFMLTTLAMFVIAVAVFAYARDGRR
                     GLELQGRPGVLRQDLGYFLVMYALAMVAGLIHVRPLHWALAVVLVVGYVFYVRRHFRA
                     PAEDQMEAEAQGEIKPLYLWAWMRRFQPSAPRWADRTSEWPSIGQVLLALVLIVGGAR
                     LFVYAVDLIGARFHVSHLVFALLVAPLATELPEKFNSVLWVRRSKDTLALGNMTGAMV
                     FQSSFPVTVGLLLTPWHLAHEALVAASIALAAGFVLYVTIRTRRRFTAPLLLAQGIFY
                     VGYVTYVLTR"
     gene            complement(2044..2997)
                     /locus_tag="VE646_09195"
     CDS             complement(2044..2997)
                     /locus_tag="VE646_09195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HZD80200.1"
                     /translation="MRRRAAVVVLGLVAWIAGSAWGTVAPPPAAQAQALLQIGQAHAG
                     YVPTLNGTSPVFILLIGSGARPDENVDHSLADSIHLLALNPAKHKATILGLPRDSWVS
                     IPGHSPNKINAAMVDGGPELLVQTVENLTGIKIDYWALTTFWGFQAMVDGVGGITVDV
                     PFAMQDHFSRSDFQPGVQRFNGRDALAFARDRHSLPEGDFGRSENGGRLMLAALANFR
                     KAFTKDPSRLLTWVASGLRNVDTNVPIDQVLSLAFTASSVNPKTVANIVAPGVTGSVG
                     TISVVNLLPGDKTLYEDLKNDGLVSKKHLPSSPNAALLGGG"
     gene            complement(2994..3626)
                     /locus_tag="VE646_09200"
     CDS             complement(2994..3626)
                     /locus_tag="VE646_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80201.1"
                     /translation="MALVALGLILFLVLRGGSGGGGILGRAQDDSIPRLRFRLSKVVP
                     VPVTDAKPSELEHAARPAASAVTKTITALYSEAFLDPGNWRGGSYDSVWTLFDDGSRS
                     AAQGEDQTLTLGPSAGDRYETVKQPKGKLSVKVLLDDKEQAATAVAIVRFETVATGKD
                     GTLTTVVSTGQYFLHPGPDGWRIYSFSVGREDEEQAPQPGPSGSPSGGSS"
     gene            complement(3796..5475)
                     /locus_tag="VE646_09205"
     CDS             complement(3796..5475)
                     /locus_tag="VE646_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZD80202.1"
                     /translation="MRRPVFRLPQRDAIRAIRLRWQRTLLLAGLTGIVTGVGVALFDY
                     VTQDLLLDNVRTLPHWLQAVMPAAGLVAAWAFLRWVAGRASPSTSDEYIRNFHDRGTR
                     LNLRPVPGRILASIATLGSGGAMGFEGPSIYLGAAVGSFLQRRFAQYFSREDAKILLV
                     AGAAAGVSAIFKTPATGALFALEVPYQQDVAARAALPAVLASATSYLTFVAFQGTTPL
                     FLVKGHPGFDTRDLAGALAIGLLCGLAARAFAWLIKQAKRLQQSLGPTRSLALGAVGL
                     AGIGLISFSAYGSPISLGPGYEAVGWITQPSRGLWLVGLLLLSRAFATAFTVGGGGAG
                     GLFIPLVVEGAVLGGLVAGVTRLTRTTLFPLVGAAAFLGAGYRTPLAGVMFVAESTGQ
                     PGFVVPGLIATVIAQLVMVRSSVTAYQYRMRGGHVEQRFRLPVTSVLTRDPYTVPPST
                     TVDDLLHRHFVRARARALPVTKDGQYLGFVTLQDVADVPREERAQRTVQDVLEKGTIE
                     GRSTWTLRQAVEAMETADVDQLPIVDGEVFRGVVTMDDILSLDEILQATEE"
     gene            5578..6963
                     /gene="nhaA"
                     /locus_tag="VE646_09210"
     CDS             5578..6963
                     /gene="nhaA"
                     /locus_tag="VE646_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HZD80203.1"
                     /translation="MMRGTGSREDDPTAQVRQTWSRSERRLPRIVLRPLQEFLHTESA
                     GGMLLLLAAVAALIWANSPWRESYEHWWHTKLAVGLGGRSIAQDLRHWVNDGLMALFF
                     LVVGLEIKRELLTGELRDRRTAVLPAVAALGGMVVPALMYLVLNAGGSGSHGWGIPMA
                     TDIAFALGVLTLAARHAPPSLKPFLLSLAIVDDIGAIIVIAISYSGGISWGSLGVASG
                     LCGAIVLLQRGHVRAVAVYLVLGAGVWLATYESGVHPTIAGVALGLLTPAVPFQRPRA
                     VSEQAHRVADETVDDPSPPDADAQHWLLLAELSREAVSPLVRMEEALHPWTSWVIIPL
                     FALANAGVTLSSASLARAVTSPIAIGIALGLVVGKVVGISLGSILAVRSGVARMPQGV
                     GWGELVGAAAVAGIGFTVSLFIAELAFEPAVLVDTAKVGILFASLVAGVVGFFLLRLR
                     PRTAQDPDGRA"
     gene            complement(6972..>7590)
                     /locus_tag="VE646_09215"
     CDS             complement(6972..>7590)
                     /locus_tag="VE646_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80204.1"
                     /translation="PGWRRRPRPSPSSASSTTAAASSSFQAGPGLLKALARHRDGDGP
                     SVGILPQALGRTNRHHTPYWSVAVYLAIAGLVVLAANGRDQELVLFYAVAVFVSFLSG
                     LLAMTRFSRREGDRFHATVNLIGSALVAFTLLVNLRRGYPIASLVAAGIIALVLYGLW
                     VRQGRPRGIAMAEAEAEREVGRPDEEEDEDRAVDRPGPGAAPAEA"
BASE COUNT         1091 a         2641 c         2753 g         1105 t
ORIGIN      
        1 gaacacgaac atcacgacca cggccacccg ccggtgggcc acggggagcg agccgaggta
       61 acgcgccggc cccaccaggt ggggctcctc ggccggggct cgggagatgc ggatcgtgta
      121 ggccacgaag atcgccacca gcaccagcga gtcccagagc gtgaccgagt gcttcaaggg
      181 aagggtcagc gagtacaccg tggcgatggc caggaaggtg acctcgaccg agtgctgccg
      241 cgtgagcacc acctcttcgt ggggacggcc cctggaccgc cagcggtacc aggcgatgaa
      301 caccaccatc gaccacccga tcccgatcag cagccggttg gcgccggtca tgttggccag
      361 ggccagggag caggcggtgt ccccgccggc ccccggagcc cggcaggctg ggccgtaggc
      421 ctgaacggcg tggccgccct tcagcgcgaa caccatgtcc accgcgtact cgggcaggac
      481 ggcgatgagg gccaacacgg cgatggccag ccccgccgag atgtccagct gcgcgacctc
      541 ggccgcccag gacagcagga acgcggcccc cacgatcgcc agcccgaaca gcacggcctg
      601 gatggcatcc gccggatgca gtccccagaa gcgaagcgcg aggccgggga gcgtcgcccc
      661 cagggcgagc gcgatcgtga cgcgctgccg ggtgggatgg acctctcgga gctcgtccct
      721 cagctcggtc acgcaggcct cctcagcccg tcgaccccgc cgaaggtctc gtccccgacc
      781 cgcgaggagc ccgccgggcc gcatgggcgg ccgtggtgac ggcggagctc gcttcggact
      841 actcccctcc gtggatctgc cacgtcatcg ggctcacggg tcgacccggg ctcccgatgc
      901 gggcagcgta acagagcccc cgggcggtcg gtggcgacgg ggtgtgggac gatgccgccc
      961 cgatggaatg gccggagctg atcgcctcgc tgttcgtgat cctcggcggc gcagagctgt
     1021 tcacgaacgg ggtggagtgg atcggcgagg gctttggtct ctccgagggg gccgtgggca
     1081 gcgtcctggc cgccgtgggg accgccctgc ccgagacggt cctgcccctg atcgccatcc
     1141 tgtccggtca cgcctccgga gagcagatcg ggatcggggc catcctgggc gcccccttca
     1201 tgctcaccac gctcgcgatg ttcgtgatcg ccgtggccgt gttcgcctac gcccgggacg
     1261 gacggcgcgg gctggagctg cagggccggc ccggggtgct ccgtcaggac ctgggctact
     1321 tcctggtgat gtacgccctg gccatggtgg cggggctcat ccacgtgcgg ccgctgcact
     1381 gggccctggc cgtggtgctg gtggtgggct acgtgttcta cgtccggcgg cacttccgcg
     1441 ccccggcgga ggaccagatg gaggccgagg cgcagggcga gatcaagccg ctctacctgt
     1501 gggcctggat gcggcggttc cagccctcgg cgccccggtg ggccgatcgc acctcggagt
     1561 ggccgagcat cggccaggtc ctgctggccc tggtgctcat cgtgggcggg gcccggctgt
     1621 tcgtctatgc cgtggacctg atcggcgctc ggttccacgt atcccacctg gtcttcgcgc
     1681 tcctggtggc gcccctggcg acggagctcc ccgagaagtt caacagcgtg ctatgggtgc
     1741 gccgatccaa ggacacgctg gccctgggca acatgaccgg ggccatggtg ttccagtcct
     1801 cgttccccgt caccgtgggg ttgctgctca cgccgtggca cctcgcgcac gaggcgctgg
     1861 tggcggccag catcgctctg gcggccgggt tcgtgctcta cgtcacgatc cggacccgtc
     1921 gccgtttcac ggcaccgctc ctgctggccc agggcatctt ctacgtgggg tacgtgacct
     1981 acgtgctcac ccggtgaccg cggctcccga gcggttcggc gcgctcgcga ggacgacgcg
     2041 ggatcaaccc ccgccgagga gcgccgcgtt ggggctcgac gggaggtgct tcttcgagac
     2101 gagcccgtcg ttcttcaggt cctcgtagag tgtcttgtcg ccgggcagga ggttcaccac
     2161 cgagatcgtg cccacggagc cggtcacgcc gggcgccacg atgttggcca ccgtcttggg
     2221 gttcacggag gaggccgtga aggcgagcga gaggacctgg tcgatcggga cgttggtgtc
     2281 gacgttgcgc agtcccgagg cgacccacgt gaggagccgg ctggggtcct tggtgaacgc
     2341 cttgcggaag ttggccaggg cggccagcat cagccgtcca ccgttctcgg agcggccgaa
     2401 gtcgccctcg ggcaggctgt ggcggtcgcg ggcgaaggcc agggcgtcgc ggccgttgaa
     2461 ccgctggacc ccgggctgga agtcggagcg ggagaagtgg tcctgcatcg cgaacggcac
     2521 gtccaccgtg atcccgccca ccccgtccac catggcctgg aacccccaga acgtggtcag
     2581 ggcccaatag tcgatcttga tcccggtgag gttctccacg gtctggacca ggagctcggg
     2641 tccaccgtcg accatggcgg cgttgatctt gttcgggctg tggcccggga tgctcaccca
     2701 cgagtcgcgg gggaggccga ggatcgtcgc cttgtgcttg gcggggttca gcgcgagcag
     2761 gtggatcgag tcggccaggg aatggtccac gttctcgtcg ggccgggccc cgctgccgat
     2821 cagcaggatg aagaccgggc tggtgccgtt cagggtgggc acgtagcccg cgtgcgcctg
     2881 gccgatctgc agcagggcct gcgcctgggc cgcggggggc ggggcgacgg tgccccaggc
     2941 ggagccggcg atccacgcca cgagccccaa gaccacgacg gccgctcggc gcctcacgag
     3001 gatccccccg acggcgatcc cgaggggccg ggctggggcg cctgctcctc gtcctcacga
     3061 ccgacgctga acgagtagat cctccagccg tccggccccg gatggaggaa gtactggccc
     3121 gtgctcacca ccgtggtcag cgtcccatcc ttgcccgtgg ccaccgtctc gaaccgcacg
     3181 atggcgacgg cggtggccgc ctgctccttg tcgtcgagga gcaccttcac cgacagcttg
     3241 cccttcggct gcttcaccgt ctcgtacctg tcccccgccg agggacccag cgtgagggtc
     3301 tgatcctccc cctgtgctgc cgagcgagaa ccgtcgtcga acagggtcca gacgctgtcg
     3361 taggagcccc cccgccagtt cccggggtcc aggaacgcct ccgagtacag cgcggtgatg
     3421 gtcttcgtca ccgcggaggc ggcgggccgg gcggcgtgct cgagctcgga gggcttcgcg
     3481 tccgtgaccg gcaccggcac gaccttcgac aggcggaagc gcagccgcgg gatcgagtcg
     3541 tcctgcgccc ggccgaggat gcccccgccg ccggagccgc ccctgagcac caggaacagg
     3601 atcagcccca gcgcgaccag cgccaccggg acaatccaca tggcgggggg gacgtgccgt
     3661 ccggcagacg cggcttcgcg atgacgaccc tcggacaaaa cgcgctggag cgtaccggga
     3721 gtgaggatgg acaacaacca ccccggtcgc acgccccgtg gccgacacgg aagctccaca
     3781 tggcggggcc tgagctcact cctcggtggc ctgcaggatc tcgtccaggc tgaggatgtc
     3841 gtccatcgtg acgacgccgc ggaacacctc gccgtccacg atcgggagct gatcgacgtc
     3901 ggcggtctcc atggcctcca cggcctgacg gagcgtccag gtgctccgtc cctcgatcgt
     3961 ccccttctcc aggacgtctt ggacggtgcg ctgcgctcgc tcttcccgcg ggacgtcggc
     4021 gacgtcctgc agggtcacga agccgaggta ctgaccgtcc ttggtcaccg gcagggcccg
     4081 ggcccgggca cggacgaagt gccggtgcag caggtcgtcc acggtggtgc tgggcggcac
     4141 cgtgtacggg tcccgggtga gtaccgaggt caccgggagc cggaagcgct gctccacgtg
     4201 cccgcctcgc atccggtact ggtacgcggt gaccgagctc cggaccatca ccagctgggc
     4261 gatcacggtg gcgatcagcc ccggaaccac gaagccgggc tgaccggtgg actccgcgac
     4321 gaacatcacg ccggccagcg gtgtgcggta gccggctccc aggaaggcgg cggcccccac
     4381 cagggggaac agggtggtgc gcgtgagccg ggtcacgccg gccaccagac ctccgaggac
     4441 ggcaccctcg acgaccagcg ggatgaacag cccaccggcc ccacccccgc cgacggtgaa
     4501 ggcggtggcg aacgcccggg acaggagcag cagcccaacc agccagagcc cccggctcgg
     4561 ctgggtgatc catcccaccg cctcgtagcc cggccccagc gagatcgggc ttccgtacgc
     4621 gctgaagctg atgagcccga tgccggccaa ccccacggcg ccgagggcca ggctccgggt
     4681 gggccccagg ctctgttgga ggcgcttggc ctgcttgatc agccaggcga acgcccgggc
     4741 ggcgagtcca cacaggagcc cgatggccag cgccccggcc aggtcacggg tgtcgaaccc
     4801 cggatggccc ttcaccagga acagcggtgt ggtgccctgg aaggccacga aggtcaggta
     4861 ggaggtggcc gaggccagca ccgcggggag cgcggcacgc gccgccacgt cctgctgata
     4921 agggacctcg agcgcgaaca gggctcccgt ggccggcgtc ttgaagatcg ccgacacccc
     4981 ggcggcggcg cccgccacca gcaggatctt cgcatcctcc cgcgagaagt actgggcgaa
     5041 gcggcgctgc aggaacgagc cgaccgccgc tcccaggtag atggacgggc cctcgaagcc
     5101 catcgcgccg cccgatccca gcgtggcgat ggaggccagg atccggcccg ggaccggccg
     5161 caggttcagg cgggtcccgc gatcgtggaa gttccggatg tactcgtcgg aggtggaggg
     5221 gctggcccgt cccgccaccc accggaggaa ggcccatgcc gccaccagtc ccgcggccgg
     5281 catgacggcc tggagccagt gagggagggt gcggacgttg tccagcagga ggtcctgtgt
     5341 gacgtagtcg aacagggcca caccgacgcc ggtgacgata cccgtgagcc cggccagcag
     5401 gagggtccgc tgccaacgca gccggatggc acggatggca tcgcgctgcg gcagcctgaa
     5461 cacgggtcgt ctcatgtacg cctcacatcg tatgcatcgg gaccccctcg ccgtggcggt
     5521 tcgggacccc cgctacgatg tcgtgtccca cgtgcggggc tcgatcggcg ggaggggatg
     5581 atgaggggaa cgggctccag ggaggacgat cccacggcgc aggtccgtca gacgtggtcg
     5641 cgatccgagc ggcggctgcc caggatcgtg ctccgcccgc tgcaggaatt ccttcacacc
     5701 gagtcggccg ggggcatgct gctgctgctg gccgcggtgg ccgcgctgat ctgggcgaac
     5761 tcgccgtggc gggagagcta cgagcactgg tggcatacga agctcgcggt cggcctgggt
     5821 ggccgatcga tcgcccagga cctgcgccac tgggtgaacg acgggctgat ggccctgttc
     5881 ttcctggtgg tggggctcga gatcaagcgc gagctcctga cgggcgagct tcgggaccgc
     5941 cggacggcgg tgctgcccgc cgtggccgcc ctgggcggga tggtcgttcc ggcgctcatg
     6001 tacctggtcc tgaacgccgg tgggtcaggc tcgcacggct gggggatccc catggccacg
     6061 gacatcgcct tcgccctcgg ggtgctgacc ctggccgccc gtcatgcacc cccgtcgctg
     6121 aagccgttcc tgctcagtct ggcgatcgtg gatgacatcg gcgcgatcat cgtgatcgcg
     6181 atctcctact cgggcggcat cagctggggg tcgctggggg tcgcgtccgg cctctgcggc
     6241 gcgatcgtcc tgctgcagcg gggccacgtc cgcgccgtcg ccgtgtacct ggtcctgggg
     6301 gcaggggtgt ggctggcgac ctacgagtcc ggcgtgcatc caacgatcgc cggggtcgcg
     6361 ctcggtctgc tcaccccggc ggtgcccttc cagcgcccac gagcggtgag cgagcaggcc
     6421 catcgggtgg ccgacgagac cgtcgacgac ccgtcgcccc cagatgccga cgcgcagcac
     6481 tggctcctgc tggccgagct cagccgggag gccgtctccc cgctggtgcg gatggaggag
     6541 gctctgcacc cctggacgag ctgggtgatc atcccgctgt tcgcgctggc gaacgcgggc
     6601 gtgacgttgt cgagcgcgtc cctggcgcga gcggtgacca gccccatcgc catcggcatc
     6661 gctctcggtc tcgtggtggg caaggtggtc gggatctcgt tggggagcat cctggcggtc
     6721 cggagcgggg tggcccggat gccgcagggg gtcggctggg gggagctcgt gggagcggcg
     6781 gcggtggccg gcatcggctt caccgtctcc ctgttcatcg ccgagctcgc cttcgagccg
     6841 gccgtcctgg tggacaccgc gaaggtcggg atcctgttcg cgtccctggt ggccggtgtg
     6901 gtcggcttct tcctgctgcg ccttcgtccc cggacggcgc aggaccccga cggtcgagcc
     6961 taggtcaccg atcaggcctc ggccggcgcg gcacccggac ccgggcggtc cacggcccgg
     7021 tcctcgtcct cctcctcatc ggggcgtccc acctcgcgct cggcctcggc ctcggccatc
     7081 gcgatgcccc gaggacggcc ctgccggacc cagagcccgt acaggaccag agcgatgatc
     7141 ccggcggcca cgagcgaggc gatcgggtac ccgcgccgca ggttcaccag gagggtgaac
     7201 gcgaccagcg ccgagccgat cagattgacc gtggcgtgga agcggtcacc ctcgcgccgg
     7261 gagaaccggg tcatcgccag gagccctgag aggaagctca cgaagaccgc aaccgcatag
     7321 aagagcacca actcctggtc ccggccgttc gcggccagga ccaccagtcc ggcgatggcc
     7381 aggtacacgg ccacgctcca gtagggcgtg tggtgccggt tggtccgtcc cagggcctgg
     7441 gggaggatgc ccacgctggg gccgtcgcca tcgcggtgcc gggccagggc cttcaagagc
     7501 ccgggaccgg cctggaacga cgagctcgcg gccgcggtcg tcgagctggc cgagctgggc
     7561 gatggccgag gacggcgcct ccaccccggt
//
LOCUS       DASQQQ010000241         3551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_14502, whole genome shotgun sequence.
ACCESSION   DASQQQ010000241 DASQQQ010000000
VERSION     DASQQQ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14502"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VE646_09220"
     CDS             complement(<1..380)
                     /locus_tag="VE646_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZD80205.1"
                     /translation="METEFRMTPALGAPPVQDGPRRESILVVDDDPFIARLLEIELRT
                     AGFEVRLASDGEQALEAAQQDCPDLVLCDVMMPNMDGFELTRRLRQDGRTATVSVIML
                     SARGLSADKLEGFSVGADDYVVKP"
     gene            597..1979
                     /locus_tag="VE646_09225"
     CDS             597..1979
                     /locus_tag="VE646_09225"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HZD80206.1"
                     /translation="MQVLESFLDAVGGTPLLRLRRVTEGVRATVLAKLEMLNPGGSVK
                     DRIGLRMVEAAERGGLLEPGGTIVEPTSGNTGHGLAIAAAIKGYKCIFVMPDKMSQEK
                     IALLRAYGAEVVITPTAVPPESPESYYRVADRLTEEIPGAFQPNQYFNQENPAAHYAT
                     TGPEIWEQTDGRVDVLVAGVGTGGTITGTARYLKERRPDLLVVGADPAGSLYSAAPGE
                     EARPYLTEGIGEDFWPETFDPSLVDRWVRVSDRDSFRMARAITRREGILVGGSSGTAV
                     HAAVQVARELDRSAVVVVILPDTGRNYLSKLYSDTWLLQYGLLERPDVVRVEEVVIAK
                     QGEIPPLVTITAHDKVRQAIDLLQTYGISQAPVVRETSAEVSRFVGSIQERDLLDRIF
                     RDPDALQADVAEIMGPPIPMVDADEAIEVAFGDLQREPAVLVAKGGTVLGVLTRSDLL
                     EFLAHRKRTP"
     gene            1985..2833
                     /locus_tag="VE646_09230"
     CDS             1985..2833
                     /locus_tag="VE646_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HZD80207.1"
                     /translation="MRSLGIDVGVRKGSDLVLLDGGRRLQDTHRHVRVEDLGPLVVAM
                     KPDVVAVDAPPSWAVSGRSRRTERELRWFGIQCFNTPSDRRMADHPFFEWMTVGFSVY
                     AAIASGYPRYRGGSVRGRAIEVFPHASAVVLTGNLPPPAVPKQRWRRTVLVGCGVDAG
                     ALRSADQVDAALAALTGLYALERRFSAPGDPREGVIVLPAATLPSHPFRRGTAAAEQA
                     GQPPLPGLGACGCGDPACMALTAREFAPGHDAKRKSALWKVARSGQDAVEELRRRRWE
                     LPPEMR"
     gene            2806..>3551
                     /locus_tag="VE646_09235"
     CDS             2806..>3551
                     /locus_tag="VE646_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019809886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HZD80208.1"
                     /translation="MGAAPGDALRFETNAIHAGQEPEPQYGAVNVPIYQTSTYAQPDV
                     GAPKRYDYARGGNPTREALQTALAALEGGRHGVSFGSGLAAETTMLLTLSPGDHVLLA
                     NDVYGGTYRLLAWVLSRWGLGFDVVDMTDLGSLRGAIRDTTRLVWVETPTNPLLKIID
                     IQAVAGVAHEAGARCAVDNTFATPYLQRPLELGADLVVHSVTKYVAGHSDLVGGAVVT
                     DDDDLAEELRFLQNAVGAVPGPMDCYLALRG"
BASE COUNT          520 a         1244 c         1268 g          519 t
ORIGIN      
        1 aacggcttga cgacgtagtc gtcggcgccc accgagaacc cttccagctt gtcggcggat
       61 agccctcggg cgctcagcat gatcacgctc accgtcgccg tccggccgtc ctgccggagc
      121 cgccgggtga gctcgaagcc gtccatgttg ggcatcatca cgtcgcacag gaccaggtcg
      181 gggcagtcct gctgggcggc ctcgagagcc tgctcgccat cggaggccag ccggacctcg
      241 aacccagccg tccgcagctc gatctccagc agtcgggcga tgaacgggtc gtcgtccacg
      301 accaggatgc tctcccggcg gggtccatcc tgcaccgggg gggctcccag ggccggcgtc
      361 atccggaact cggtctccac cgggagtgga tcggcgcgca gggccgtccg gtgtagccgg
      421 ctggaaggat caactccccc tccctgtggg tcctcccgcg gtccacgtgg gccttgaggt
      481 cgagggccgc tccaactgct cccgatgcac gctcgctcac gccgctctcc tcatcggcgg
      541 gacgctcggg caagagtgaa gcacaatagg ggcccggagc cgagcaaggg ggtgccgtgc
      601 aggtcttgga gagcttcctg gacgccgttg ggggcacccc gctgctccgt cttcggcggg
      661 tcaccgaggg cgtcagggcc accgtcctgg cgaagctcga gatgctgaac ccggggggga
      721 gcgtcaagga ccgcatcggc cttcggatgg tcgaggcggc cgagcgcggg ggcctgctgg
      781 agccgggcgg cacgatcgtc gagcccacct cgggcaacac gggccacggt ctcgccatcg
      841 cggccgcgat caagggctac aagtgcatct tcgtgatgcc ggacaagatg agccaagaga
      901 agatcgccct gctgcgggcc tacggtgccg aggtggtcat cacccccacc gcggtgccgc
      961 ccgagtcccc cgagtcctac taccgggtgg ccgaccgcct gaccgaggag atccccggcg
     1021 ccttccagcc caaccagtac ttcaaccagg agaaccctgc cgcgcactac gccaccacgg
     1081 gccccgagat ctgggagcag accgatggcc gggtcgacgt cctggtggcc ggcgtgggca
     1141 ccggcgggac catcacgggc accgccaggt acctgaagga acgcaggcca gacctgttgg
     1201 tggtgggggc cgatcccgcg ggttccctgt actcggccgc gcccggggag gaggctcgcc
     1261 cctacctcac cgaggggatc ggggaggact tctggccgga gacgttcgac ccgtccctgg
     1321 tggaccgctg ggtcagggtc tccgaccgcg actcgttccg catggcccgc gccatcaccc
     1381 gccgagaggg catcctggtg ggtggctcct cgggcaccgc cgtgcacgcg gccgtgcagg
     1441 tggcccgcga gctcgaccgg tcggccgtcg tggtcgtgat cctgccggac accggacgca
     1501 attacctctc gaagctgtac tcggacacgt ggctactcca gtacggcctg ctcgaacgac
     1561 cggacgtggt ccgggtggag gaggtcgtga tcgccaaaca gggggagatc cccccgctcg
     1621 tcacgatcac cgcacacgac aaggtccgtc aggccatcga cctgctgcag acgtacggga
     1681 tctcgcaggc gcccgtggtg cgggagacct cggccgaggt ttcgcggttc gtgggctcga
     1741 tccaggagcg cgacctgctg gaccggatct tccgggaccc cgatgccctc caggccgacg
     1801 tggcggagat catgggtccg cccatcccga tggtggacgc cgacgaggcc atcgaggtcg
     1861 cgttcggcga cctgcagcgg gagccggccg tgctggtggc caagggggga acggtcctgg
     1921 gcgtcctgac caggagcgac ctcctggagt tcctggcgca ccggaaacgg acgccctgag
     1981 ggccgtgcgc tcgctgggga tcgacgtcgg ggttcgcaag ggttcggacc tggtcctgct
     2041 cgacggaggg cggcgcctcc aggacacgca tcgccacgtc cgggtggagg acctggggcc
     2101 gctggtcgtc gcgatgaagc ccgacgtggt ggccgtggac gccccgccgt cctgggcggt
     2161 gagcggacgg tcccgtcgta ccgagcgcga gctccggtgg ttcgggatcc agtgcttcaa
     2221 cacgccctcg gaccgacgga tggccgatca cccgttcttc gagtggatga cggtaggctt
     2281 ctccgtgtac gcggcgatcg cctcgggcta cccgaggtac cggggcggat cggtgcgggg
     2341 ccgagccatc gaggtcttcc cacacgcgtc ggcggtggtg ctcacgggga acctgcctcc
     2401 gcccgcggtc cccaagcagc gctggcgtcg cacggtgctc gtgggatgcg gcgtggatgc
     2461 gggtgcgctt cgctccgccg accaggtcga tgcggccctg gccgcgctca ccgggctcta
     2521 tgccctcgag cggcgcttct cggcgcccgg tgacccccgt gagggcgtga tcgtgcttcc
     2581 cgccgcgaca ctgccctcgc accccttccg gcgcggaacc gcggctgccg agcaggccgg
     2641 ccagcccccc ctgccgggct tgggagcgtg cggctgcggc gaccccgcgt gcatggcgct
     2701 caccgcgcgc gagttcgccc ccggacacga cgccaagcgc aagtcggcgc tctggaaggt
     2761 ggcccgctcg ggccaggacg ccgtcgagga gctgaggagg agacgatggg agctgccccc
     2821 ggagatgcgt tgaggttcga gacgaacgcg atccacgcgg ggcaggagcc cgaacctcag
     2881 tacggcgcgg tcaacgttcc catctaccag acctcgacct acgcgcaacc ggacgtcggg
     2941 gcgccgaagc gatacgacta cgcgcgagga ggcaacccca cgcgagaagc gctccagacc
     3001 gcactggccg cgctcgaggg cggccgccac ggcgtgtcct tcggctcggg gctcgcggcc
     3061 gagacgacga tgctcctgac cctgtcgccc ggcgaccacg tcctgctggc caacgacgtc
     3121 tacgggggga cctaccgcct gctggcgtgg gtgctgtcca ggtggggctt gggattcgac
     3181 gtggtggaca tgaccgacct gggatcgctg cgaggggcga tccgggacac caccaggctc
     3241 gtgtgggtgg agacccccac caaccccctc ctcaagatca tcgatatcca ggctgtggcg
     3301 ggcgtggccc acgaggccgg ggcgcggtgc gcggtggaca acacgttcgc caccccctac
     3361 ctgcagcgcc ccctcgagct cggggccgac ctcgtcgtcc actccgtcac caagtacgtg
     3421 gcgggacact ccgacctggt cgggggggcc gtggtcaccg acgacgatga cctggccgag
     3481 gagctgcgat tcctccagaa cgccgtgggc gccgtccccg ggccgatgga ctgctacctc
     3541 gcgctccggg g
//
LOCUS       DASQQQ010000242         2983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_157586, whole genome shotgun sequence.
ACCESSION   DASQQQ010000242 DASQQQ010000000
VERSION     DASQQQ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157586"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VE646_09240"
     CDS             complement(<1..263)
                     /locus_tag="VE646_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014359635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZD80209.1"
                     /translation="MDRLLVTGGVRLRGSIPVSGAKNSALKLMAASLLAEGRTVLHNV
                     PRIQDCHTMADVLEHLGAGIGWDRGTVAIDAVPVRSAEAPYELV"
     gene            complement(307..705)
                     /locus_tag="VE646_09245"
     CDS             complement(307..705)
                     /locus_tag="VE646_09245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HZD80210.1"
                     /translation="MSVEVHVVTPEREVWSGQAEMVIARGVDGEVGILGGHAPLLVRL
                     AIGHLRVIRDGQEDFVSVVDGGFLHVSIPESGITRVDVLGSNAALLSEIDVELERRRQ
                     EEMERRIEAGDDAEAHAELAKALARLSLRG"
     gene            complement(708..2135)
                     /gene="atpD"
                     /locus_tag="VE646_09250"
     CDS             complement(708..2135)
                     /gene="atpD"
                     /locus_tag="VE646_09250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZD80211.1"
                     /translation="MTDENQHASGRVVRVIGPVIDVAFPPGELPEIDTALRVDVTLDG
                     ETTTITCEVAQHIGDSTIRAIALRPTDGLVRGTVVDNTGSPIRVPVGEGVLGHVYNVL
                     GQPLDADADAIHPSDWWPIHREAPPFEELEPKSVMLETGIKVIDLLEPYVQGGKIGMF
                     GGAGVGKTVIIQEMIRRQAEQHGGVSVFAGVGERTREGNDLFIDMTESGVIEKTALVF
                     GQMDEPPGVRLRVALSALTMAEYFRDVERKDVLLFIDNIFRFTQAGSEVSTLLGRMPS
                     AVGYQPTLADEMGELQERITSTRGRSITSLQAIYVPADDITDPAPHTAFAHLDATTVL
                     SRDIAALGIYPAVDPLDSTSRILDARYVGEEHYRVARRVQEILQRNKDLQDIIAILGV
                     DELSEEDKVIVARARKIQRFLSQPFFVAEQFTGLPGKYVPIDETIASFDGICEGQYDH
                     LPEQAFFLVGGIEEALEHAKRLEAA"
     gene            complement(2132..>2983)
                     /locus_tag="VE646_09255"
     CDS             complement(2132..>2983)
                     /locus_tag="VE646_09255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HZD80212.1"
                     /translation="TMKITRAMELIAASRIVKAQQRVEAARPYAELLTRAMEDVARQT
                     GSLVHPLLEHRPEPRRAGVLVVTSDRGLAGAYSTNVIRRAEALGREIAGRGLEQALYL
                     TGKKGQSYFRFRGVPAEAVWTGFSEVPPYSAAETVGGRLIEDFAERRIDELHAVYTDF
                     RSAFSMRVTVNRFLPIAQEEVLRRELGKPHPEYLFEPAPQQILDELLPQYVITKVFHA
                     FLESAASENAARRRAMKSATDNAEELIRIYTRQANRARQDEITTEISEIVGGAEALAQ
                     ASGDGQP"
BASE COUNT          440 a         1079 c          986 g          478 t
ORIGIN      
        1 acgagctcgt acggggcttc ggccgagcgg accgggacgg cgtcgatcgc cacggtcccc
       61 cggtcccatc cgatgcccgc gccgagatgc tccaaaacgt cggccatggt gtggcagtcc
      121 tggatcctgg ggacgttgtg cagcaccgtc cgtccttcgg ccagcagcga agccgccatc
      181 agcttgagcg cggagttctt ggccccggac acggggatcg agccccggag ccgaacccct
      241 cccgtcacca ggagtcgatc catggtgccc ccatcctagg cccgagggtg gcgccccgag
      301 cgcggttcat cctcgcaggc tgagcctggc caatgccttg gccagctcgg cgtgcgcctc
      361 cgcgtcgtcg ccggcctcga tccggcgctc catctcctcc tggcgccgcc gctcgagctc
      421 cacgtcgatc tccgacagca gggcggcgtt cgagcccagc acgtcgaccc gcgtgatgcc
      481 ggactcgggg atcgagacgt gcaagaaccc cccgtccacc accgacacga agtcctcctg
      541 gccgtcccgg atgacccgca ggtgcccgat cgcgaggcgg accagcagcg gcgcgtggcc
      601 gcccaggatc cccacctccc catccacacc gcgggcgatc accatctcgg cctgcccgga
      661 ccagacctcg cgctcgggtg tgacgacgtg gacctcgacc gacaccgcta cgcggcctcc
      721 agacgcttgg cgtgctccaa ggcctcctcg atccccccca ccaggaagaa ggcctgctcg
      781 ggcaggtggt cgtactggcc ctcgcagatc ccgtcgaacg aagcgatggt ctcgtcgatc
      841 ggcacgtact tgccgggcag gccggtgaat tgctcggcca cgaagaacgg ctgcgacagg
      901 aaccgctgga tcttgcgggc ccgggccacg atgaccttgt cctcctccga cagctcgtcc
      961 acccccagga tggcgatgat gtcctggagg tccttgttcc gctgcagtat ctcctggacg
     1021 cgccgggcca cccggtagtg ctcctcgccc acgtaccggg catcgaggat ccgggacgtg
     1081 gagtccaacg gatcgaccgc cgggtagatg ccgagggcgg cgatgtcacg cgagagcacg
     1141 gtggtggcat ccaggtgggc gaacgccgtg tgcggcgccg ggtccgtgat gtcgtcggcc
     1201 gggacgtaga tcgcctgcag cgatgtgatc gagcgtccgc gggtggaggt gatccgctcc
     1261 tgcagctctc ccatctcatc ggccagcgtg ggctggtagc ccacggccga gggcatccgc
     1321 ccgagcaggg tggagacctc ggagccggcc tgcgtgaacc ggaagatgtt gtcgatgaac
     1381 agcagcacgt ccttgcgctc gacatcgcgg aagtactcgg ccatggtgag ggcggacagg
     1441 gccacccgca ggcgcacgcc gggcggctcg tccatctggc cgaagaccag cgcggtcttc
     1501 tcgatgactc cggactcggt catgtcgatg aacaggtcgt tgccctcgcg ggtgcgctca
     1561 cccacgcccg cgaacaccga caccccgccg tgctgctcgg cctggcggcg gatcatctcc
     1621 tggatgatga ccgtcttgcc cacgccggcc ccgccgaaca tcccgatctt ccccccctgc
     1681 acgtacggct cgagcagatc gatgaccttg atcccggtct cgagcatcac gctcttgggc
     1741 tcgagctcct cgaacggcgg cgcctcccgg tggatcggcc accagtcgct ggggtggatc
     1801 gcatccgcgt cggcgtccag cggctggccg aggacgttgt agacgtggcc caggacgccc
     1861 tcccccaccg gcacccggat cggcgatccc gtgttgtcca ccaccgtgcc ccgtaccagc
     1921 ccatcggtgg ggcggagcgc gatggcccgg atggtcgaat cgccgatgtg ctgcgcgacc
     1981 tcgcacgtga tcgtggtggt ctcaccgtcg agcgtgacgt cgaccctcag ggccgtgtcg
     2041 atctcgggca actccccggg tgggaaggcc acgtcgatca ccggtccgat cacccggacc
     2101 acccggccgg aggcgtgctg gttctcgtcc gtcatggttg tccatctccc gatgcctggg
     2161 ccagggcttc cgccccgccg acgatctcgc tgatctcggt cgtgatctcg tcctgccggg
     2221 cgcggttggc ctgccgcgtg tagatcctga ttagctcctc agcgttgtcc gtggccgact
     2281 tcatggcccg ccgccgggcg gcgttctcgg acgcggcgga ctcgaggaag gcgtggaaga
     2341 ccttcgtgat gacgtactgg gggaggagct cgtccaggat ctgttggggg gcgggctcga
     2401 acaggtactc gggatgcggc ttgcccagct cccgccgcag cacctcttcc tgggcgatcg
     2461 gcaggaaccg gttgaccgtg acccgcatgg agaacgcgga gcggaaatcc gtgtacacgg
     2521 cgtgcagctc gtcgatccgc cgctccgcga agtcctcgat cagccggcca cccacggtct
     2581 cggccgccga gtacgggggc acctcgctga acccggtcca cacagcctcc gccggaacac
     2641 cgcgaaaccg gaagtacgac tgccccttct tgccggtcag gtacagcgcc tgctcgaggc
     2701 cccggcccgc gatctcgcga cccagcgcct ccgcccggcg gatcacgtta gtgctgtacg
     2761 cgccggccag accacggtcc gaggtcacca ccagcacgcc cgcccgtcgg ggctccggcc
     2821 ggtgctcgag caagggatgg accagggagc cggtctgcct ggccacgtcc tccatggccc
     2881 gggtcaacag ctccgcgtag gggcgcgccg cctccacgcg ctgctgggcc ttgacgatcc
     2941 gggaggcggc gatcagctcc atggcgcggg tgatcttcat ggt
//
LOCUS       DASQQQ010000243         6510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_44259, whole genome shotgun sequence.
ACCESSION   DASQQQ010000243 DASQQQ010000000
VERSION     DASQQQ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6510
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44259"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VE646_09260"
     CDS             complement(<1..235)
                     /locus_tag="VE646_09260"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683425.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HZD80213.1"
                     /translation="MDGEGTTSGPREVRAPRGTAITCRGWPQEAAMRMLMNNLDPEVA
                     ERPQDLVVYGGTGRAARSWGSFDAIVKALRDLAD"
     gene            complement(331..666)
                     /locus_tag="VE646_09265"
     CDS             complement(331..666)
                     /locus_tag="VE646_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80214.1"
                     /translation="MIGLALILLGLGAGLIAIDLLIENVAPASYELTLFGAVIGHVSG
                     RAIVLLLMLVAAASSSIVTYGITVLRRERAALKAREAARTDQILEAHRKLWGSHIEDL
                     DGPGEPVRA"
     gene            1216..1947
                     /locus_tag="VE646_09270"
                     /pseudo
     CDS             1216..1947
                     /locus_tag="VE646_09270"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
     gene            2110..3105
                     /locus_tag="VE646_09275"
     CDS             2110..3105
                     /locus_tag="VE646_09275"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZD80215.1"
                     /translation="MSGPLLLVVGLPEGQPAPALARVAAAVTADPDVQAITQPALDPS
                     GRTAVLSAIPRSGPGTEATGDLVRRLRDRDLPPVERTTRVTVLVGGETAMYLDLRSLV
                     VGRLLLLIAVVIALSFLLLMLVFRSILVPIKAAVLNALSICAAYGAVVAVFQWGWGAQ
                     LLGLEHAEEVVSFVPMVMFAILFGLSMDYEVFLLSRIREEYLVDRNTVESVAMGIRST
                     ARVITSAALVMIAVFLSFTLNESPVVKMIGFGLAVAVLVDATVLRIVLVPATMVLLGH
                     ANWWLPRWLVPILPNLHIGGEAAPPEIPTPQRPEPAIPITAPLVEPGRSKDRHPV"
     gene            complement(3126..3632)
                     /locus_tag="VE646_09280"
     CDS             complement(3126..3632)
                     /locus_tag="VE646_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014444029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HZD80216.1"
                     /translation="MTHPVDAVEHPMPPYVAVCGAAEPTAQEAEWAQEIGRGLAAAGA
                     VVVCGGLGGVMDAAARGAESAGGTSLGILPGSDRSEASTHLTVAIPTGLGEARNALVV
                     RSADVVIAVGGEFGTLSEIALALKRGTPVVGLATWRVAKARPAVDPIVRVEDPQEAVA
                     AALELARS"
     gene            complement(3629..5893)
                     /locus_tag="VE646_09285"
     CDS             complement(3629..5893)
                     /locus_tag="VE646_09285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZD80217.1"
                     /translation="MDPLELLRGSAETGGDPLTHLRQIPARGDEPLAFPRDLPEILVE
                     RLGLLGVRGLYAHQRRALERVRGGHDVMIATGTASGKTLVYNLAFAVDALNDPKRTAL
                     YLYPTKALARDQLRQIRELRLPQLKAAVYDGDTPRAERPLIRRNANLVLTNPDMLHAS
                     LLPDHPRWADFFLRLSLVVVDEAHVCRGVFGSHVSMVLRRLRRLVAHYGGEPRFVLSS
                     ATVGNPAELAERLVGVPFEAVTEDASPSGGKTFALWNPPVIDEESGARRSALSDASAI
                     MARLAEHEVRSIGFTRSRRAAELLAEFTRRALPSPLRERVKSYRAGYLSEDRRELERQ
                     LAAEELLAVASTNALELGIDIGTLDAAILVGYPGTRASMWQQAGRAGRRADRSLAVLV
                     AQDDPLDQYLVSHPADLFDAPPEAAVIDPRNPFILEPHLRCAAAEHPLADEELAFFGP
                     DAAQAAARLETAGALVRRRGMLHHAGRGSPHRAVDIRSASGDLFQIVIEETGELLGTV
                     DESRAYYHVHPGAVYLHQGEQFEVLELDLVGRVALVRRSDPDFYTQSRDTTDIAVVGV
                     LDETVTGDVPTFYGTVRVTNQVVAFVRKLVATGETLELVPLALPAQSLETKAVWWAVP
                     TDLVERAAVTRAQLPGAAHAAEHAVIGLLPLVATCDRWDVGGVSTAYHPDTDSCAIFV
                     YDGYPGGAGIAERGFRSARRLLEATLETVRGCPCGNGCPACVQSPKCGNGNEPLDKAG
                     AVSLLAAMLGRTWG"
     gene            complement(5938..>6510)
                     /locus_tag="VE646_09290"
     CDS             complement(5938..>6510)
                     /locus_tag="VE646_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/calcium exchanger protein"
                     /protein_id="HZD80218.1"
                     /translation="AVILLVAEHFADALVATGRDFGISEFLLVQWLAPLASEAPELLV
                     AGLYAWRLNTNAGLGTLVSSKVNQWTLLVGTLPIVFAVGVGGFHGLPIDPHQREELLL
                     TAAQSYFALAVLATLSISTGEAVMLFGLFWAQFVIGAVVPESFGGRELIIVAVAYLVL
                     GSIIFVRHRDRLPGLVRDGVLTPYRHLTRE"
BASE COUNT          884 a         2340 c         2332 g          954 t
ORIGIN      
        1 cgtcggccag gtcacgcagg gccttcacga tcgcgtcgaa gctcccccag ctgcgggcgg
       61 cccgcccggt gcccccgtag accaccaggt cctggggccg ctcggccacc tcggggtcca
      121 ggttgttcat cagcatgcgc atggccgcct cctgcggcca cccacggcag gtgatggccg
      181 tcccgcgggg tgcgcggacc tcgcggggac ccgaggtggt tccctcaccg tccatcgtcg
      241 cgctcctcct tcccacggca tgcggagcgc cgaccggcgg aggcgtggtg tcgcgtggga
      301 gccggtagcg cggccatgcc gggacccatg ctacgcgcgg accggttcgc cgggcccgtc
      361 gaggtcctcg atgtggctcc cccagagttt ccgatgcgcc tccaggatct ggtccgtcct
      421 ggccgcttcc cgggccttca gggcggcccg ctcccggcgc agcacggtga tgccgtaggt
      481 cacgatcgat gatgaagcgg cagccaccag catcagcagc aggacgatgg cccgccccga
      541 gacatgaccg atcacagcgc cgaacagggt gagctcgtac gaggcgggcg cgacgttctc
      601 gatcagcaga tcgatggcga tcagcccggc ccccagcccc aggaggatca gggcgagacc
      661 gatcatctat ctagaggtcc ttcctggggg gaagcccgga ccgcaaggag agcttctcgt
      721 gccggccggc gcgcggtcaa ggcccgcgga cgcatccgac cgccgcgctc atcgtgcggg
      781 aggcccaccg gagcgaggcc gaacgacccc ccgacgggtc cgatcgacgt tcctaccatc
      841 ggagcgggat gtccccttga gcccgcgtcc gcggttgacg tcacggctgg atcgattggg
      901 ccgagcctca gcccgtcacc ggctgctcgt ggtcgtcgtc tggatcctgg ccgcgggggc
      961 catcttctcg tggggacgga ccgtggggcc gagcttcgtg gacgacttcc ggatccccgg
     1021 cgcggaggct cagcgggcca cggatctgct cacctcccgc tcccccagtt gcgccggggc
     1081 gaccgccacg atcgtgttcc gggcccggac cggcacggtg gccgagccgg ccatcctcgg
     1141 agagatccga gcagccttgt cccgggtcgc cgccctcccg cacgtagcgg tcgtgggcga
     1201 tcccgtgcag ccgaagccgg catatcgccg gacggcacca tcgccttcac cacggtgcgc
     1261 tacgagcagg gcctctccca gctcggtcgg cccgccgccg acgatctgat cgaggcggtc
     1321 cggccggccc ggtcggcgac cctgcaggtg gaggtgggag gtcctctcat ccggttcacc
     1381 catctgcccg agccgcgggg gtcggaggtc atcgggctca cggccgccgt gatcgtcttg
     1441 ttcctggcct tcggctccct ggtggcgatg gggctgccga tcctgacggc cctgatcgga
     1501 ctggggatct cgctggcgct ggtctggctt ctgtcgcacc tgggcgacat ccccaccctg
     1561 acaccaacgc tagccacgat gctcggcctg ggggtcggca tcgactacgc gctgttcatc
     1621 gtcacccggt tccgcgagga gctgggtcga gggttggagt cgatcgaggc cgcgggccgc
     1681 gcgaccggca ccgcggggca ggccgtcctg ttcgccgggg cgaccgtgat cgtcgcgatc
     1741 tgtggactgt gggtctcggg gatgccgttc atcggcctga tagggtcggt ggcctcgaac
     1801 gcggtggcgg tggcggtcct cgcggcactg accctcctcc cggcgctcct ggggctgatc
     1861 ggacggcgga tcgacgcgct cgggacccgc tcgctgcggc ggggcgtggc ggacggaggc
     1921 gtatggggcc gctgggcccg aaccgtcgcc gatcgcccct ggcgctcgct gctcgccggg
     1981 ctggcggtgg tgtcggtgct ggtggcgccc gtggtgtcga tgcggctggg cctggacgtc
     2041 ggggtcgcgg cgaagggctc gacccaggag cgggcggcgt ccctgctcac ccgggggttc
     2101 ggtccgggga tgagcggtcc tcttctgctg gtggtggggc tccccgaagg ccagcccgcc
     2161 ccggccttgg cacgcgtcgc ggcggccgtg acggccgacc cggacgtgca ggcgatcacc
     2221 cagccggcac tggacccgag cggtcggacc gccgtcctct ccgcgatccc ccgctcgggg
     2281 cccgggacgg aggcgaccgg cgatctggtc cgacggcttc gggacaggga tctcccgccc
     2341 gtcgagcgga ccacgcgggt gaccgtgctg gtgggtggcg agaccgcgat gtacctggac
     2401 ctccgctcct tggtggtggg gcgtctcctg ctgctgatcg cggtggtcat cgccctgtcc
     2461 ttcctcctgc tgatgctggt cttccgctcg atcctcgtgc ccatcaaggc cgcggtgctg
     2521 aacgcgctct cgatctgcgc ggcctacggc gcagtggtgg cggtgttcca gtgggggtgg
     2581 ggggcgcagc tgctcgggct ggagcacgcc gaggaggtgg tgtccttcgt gccgatggtg
     2641 atgttcgcga tcctgttcgg cctgtcgatg gactacgagg tcttcctcct gtcgcgcatc
     2701 cgcgaggagt acctcgtgga tcggaacacc gtggagagcg tcgcgatggg gatccgctcg
     2761 accgcccggg tcatcacgtc cgctgccctg gtgatgatcg ccgtgttcct ctccttcacg
     2821 ctgaacgaga gcccggtggt caagatgatc gggttcggcc tcgccgtggc cgtcctcgtg
     2881 gacgccacgg tgctccgcat cgtgctggtg cccgccacga tggtgctgct gggacatgcg
     2941 aactggtggc tgccccgatg gctggtcccg atcctgccga acctccacat cggcggggag
     3001 gccgccccgc ccgagatccc aacgccccag cggccggagc cggccatccc catcaccgca
     3061 ccgctcgtcg aaccgggccg atccaaggat cgccatccgg tctgatgggc ggcggccgga
     3121 gggggtcagc tccgggccag ctccagcgcc gccgccacgg cctcctgcgg gtcctccacg
     3181 cggacgatcg ggtcgacggc cggtcgagcc ttggccactc gccaggtggc caggcccacc
     3241 accggcgttc cgcgcttcag ggccagggcg atctcggaga gcgttccgaa ctcgccgccc
     3301 accgcgatca ccacgtcggc ggaccgcacc acgagggcgt tccgggcttc gcccaggccg
     3361 gtggggatcg ccacggtgag gtgggtgctg gcctcggagc ggtcactgcc ggggaggatc
     3421 cccagggacg tccctccggc cgattcggcc cctcgggccg cggcgtccat caccccgccc
     3481 agcccgccgc agaccaccac ggccccggcc gcggccagcc cccgaccgat ctcctgagcc
     3541 cactccgcct cctgcgctgt aggctccgcc gctccgcaca cggcgacgta cggtggcatg
     3601 gggtgctcca ccgcatcgac aggatgggtc atccccacgt cctccccagc atcgccgcca
     3661 ggagcgagac cgctccggcc ttgtccaggg gctcgttgcc gttcccgcac ttgggggact
     3721 ggacgcaggc cgggcagcca ttcccgcagg ggcatcctcg caccgtctcc agggtcgcct
     3781 ccagcagccg acgcgcggat cggaagccac gctccgcgat gccggcgccc cccgggtagc
     3841 cgtcgtagac gaagatcgcg caggagtcgg tgtccggatg gtaggcggtg gagaccccgc
     3901 cgacgtccca gcggtcgcac gtggcgacca ggggcaggag gccgatcacc gcgtgctccg
     3961 ccgcatgggc cgcgcccggg agctgggccc gggtcacggc ggcgcgctcg acgaggtcgg
     4021 tggggacggc ccaccagacc gccttcgtct ccaggctctg cgcaggcagg gcgagcggga
     4081 ccaactcgag cgtctcgccg gtggccacca gcttccggac gaaggccacc acctggttcg
     4141 tgacccgaac cgtgccgtag aaggtgggga cgtcgcccgt caccgtctca tccaagacgc
     4201 ccaccaccgc gatgtcggtg gtgtcccggg actgcgtgta gaagtcgggg tcggagcggc
     4261 ggaccagggc cacgcgcccc accaggtcga gctcgaggac ctcgaactgc tctccctggt
     4321 gcaggtacac ggctcccggg tgcacgtggt agtaggcgcg ggactcgtcc accgtcccca
     4381 gcagctcgcc cgtctcctcg atcacgatct ggaacaggtc accggaggca ctgcggatgt
     4441 ccaccgcccg gtgcggtgag ccgcggccgg cgtgatgcag catcccgcgg cggcggacca
     4501 gcgccccggc cgtctctagc cgtgcggccg cctgggccgc gtccggcccg aagaacgcga
     4561 gctcttcgtc ggccaggggg tgctcggcgg ccgcacagcg aaggtggggc tcgaggatga
     4621 acgggttccg cggatcgatc acggcggcct cgggcggggc atcgaacagg tcggccgggt
     4681 gcgagacgag gtactggtcc aacgggtcgt cctgcgccac cagcaccgcc agggagcgat
     4741 cggcacgtcg accggcgcga ccggcctgct gccacatgga ggcgcgggtc cccgggtagc
     4801 cgaccaggat cgcggcgtcg agcgtgccga tgtcgatgcc gagctcgagc gcgttcgtgg
     4861 aggccacggc cagcagctcc tcggccgcca gctgccgctc cagctcacgg cggtcctcgg
     4921 acagataccc tgcccgatag gacttcaccc gctcgcggag cggggacggc agcgcgcgcc
     4981 gggtgaactc ggccagcagc tccgcggccc ggcgggaccg ggtgaacccg atcgaccgca
     5041 cctcgtgctc ggccagtcga gccatgatcg ccgaggcgtc cgagagggcg ctccggcgcg
     5101 cccccgactc ctcatcgatc actggggggt tccagagggc gaaggtcttg ccacccgagg
     5161 gcgaggcgtc ctcggtcacg gcctcgaagg ggaccccgac cagtcgctcc gccagctcgg
     5221 ccgggttccc caccgtggcg ctcgagagga cgaaccgcgg ctcgcctccg tagtgggcca
     5281 ccagccgacg aagccgccgc agcaccatcg acacgtgcga tccgaacacc cctcggcata
     5341 cgtgggcctc gtccaccacc acgagcgaca agcggaggaa gaagtccgcc catcgcgggt
     5401 ggtccggcag caggctcgcg tgcagcatgt ccgggttggt cagcaccagg ttggcgttgc
     5461 ggcggatcag gggtcgctcg gcccgcgggg tgtcgccgtc gtagacagcc gccttgagct
     5521 gcggcagccg gagctcacgg atctgccgca gctggtcccg ggccagggcc ttggtcgggt
     5581 acaggtacag cgccgtgcgc ttcgggtcgt tcagcgcatc tacggcgaag gccaggttgt
     5641 acacgagcgt cttgccgctg gccgtccccg tggcgatcat gacgtcgtga cccccgcgca
     5701 cccgctccag tgcccggcgc tggtgcgcgt acaagcctcg cacgccgagc agcccgagcc
     5761 gttccaccag gatctccggc aggtcgcgcg ggaacgcgag gggttcgtcc cctctggccg
     5821 ggatctggcg caggtgggtg agcgggtctc cgccggtctc ggcggagcct cgcagcagct
     5881 ccaggggatc catccagccg atgctaccgg cggctccggt gcaccgggcg tgcccgctca
     5941 ctcccgggtc aggtgccggt agggcgtgag gacgccgtcg cgcaccagcc cggggaggcg
     6001 atcgcggtgc cggacgaaga tgatggagcc cagcaccagg taggccacgg ccacgatgat
     6061 cagctctcga ccgccgaagg actcgggcac cacggccccg atcacgaact gggcccagaa
     6121 cagcccgaac agcatcaccg cctccccggt ggagatggac agcgtggcta ggacggcgag
     6181 cgcgaagtac gactgtgccg cggtcaggag cagctcctcc cgctgatgcg gatcgatggg
     6241 caaaccgtgg aaccctccca cgccgaccgc gaacacgatc gggagggttc ccaccagcag
     6301 ggtccactgg ttcaccttgg aggacacgag cgtgccgagg ccggcgttcg tgttgagccg
     6361 ccaggcgtac aggccggcca ccagcagctc gggcgcctcc gaggcgagcg gggcgagcca
     6421 ctgcaccagc aggaactcgc tgataccgaa gtcacggccg gtggccacca gcgcatcggc
     6481 gaagtgctcg gccaccagga ggatcaccgc
//
LOCUS       DASQQQ010000244         3482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_214464, whole genome shotgun sequence.
ACCESSION   DASQQQ010000244 DASQQQ010000000
VERSION     DASQQQ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214464"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1463
                     /locus_tag="VE646_09295"
     CDS             <1..1463
                     /locus_tag="VE646_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZD80219.1"
                     /translation="HPPAGGLEMMQCHLSDAYALPEYERIVSEYARSHPDVEWILGGG
                     WSMDVFPGGTPTADALDRLVPDRPVYLPNRDGHSAWVNSKALELAGITAETSDPPDGR
                     VERGLDGAPTGTLHEGAMDLVEKLIPETTEAEWEEGLRVAQRYLHSLGITAWQDAIVG
                     GSFDTFDAYLACAGRGELTARVVGSLWWDRHRGQEQIEEMIDRRARGDVGRFRATSVK
                     IMQDGVVETFSAAVLDPYLDRDGRPTGNRGISFVDPEALKGYVTRLDAEGFQVHFHAL
                     AERAVREALDAIEAARRSNGPSDGRHHLAHIQVVHPDDIPRFRQLNVVANAQPLWAMN
                     EGQMRNLTIPFLGPERSTWQYPFASLVRSGAVLAMGSDWSVSSPNPLWEMHVAVNRTG
                     FPGYEYGDRDDELLEPFLPRERLDLPTALAAFTMGSAYVNHLDHLTGSIEVGKLADLV
                     VLDRNPFEQPSEELYATTVQLTLVEGERVFAEGDLA"
     gene            1631..2785
                     /locus_tag="VE646_09300"
     CDS             1631..2785
                     /locus_tag="VE646_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009604667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HZD80220.1"
                     /translation="MTFDMSLVQRGARNRRTPYYEATQKHDPKGFTIYNHMYFPIRFD
                     TFEAEFDALLTGVTLWDVSVERCLEISGPDGFAFAQLLTPRDLSRCAVGQGKYVLICD
                     SDGGIVNDPVLTRMAENTFWFALASSDALLFARGLKNAYPQLDVTIQETDVAPLQIQG
                     PRSKDLMAKLVGPEILDLRYYFWTEAKIAGAPVIVTRTGWTSEVGYEVYLTDTSKGTE
                     VYDAIMEAGQPFGIRPTGPSDIRRIEGGIFNWGADMTYENNAFEMGLERLVDLDSLPD
                     DRSISVAAYRRIREQGVRRRINGVELDGEPFPGLNDIKWPVVEGDARIGKVTSAIYSP
                     RLEKNIGYAWLPADRSSAGTRASVRTQWGTRTATVVPMPFVDPSKQIPVS"
     gene            2940..>3482
                     /locus_tag="VE646_09305"
     CDS             2940..>3482
                     /locus_tag="VE646_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HZD80221.1"
                     /translation="MVNERREMLEKSATIYFGPWYRRSPFFDATRRAGCTAYDVYNHM
                     LLPAYFDDPETEYWALLNDVTVWDVAVERTVQVSGPDADRLMDLLTCRDLTRCAVKQG
                     KYMIVTAPDGGIVNDPVLLHVGENRWWMQCADSDAGLYALGVASRSGLDVEVSYPDAY
                     PMQVQGARAGKTLEKLVGAAV"
BASE COUNT          553 a         1159 c         1253 g          517 t
ORIGIN      
        1 ctcacccgcc ggcaggcggg ctggagatga tgcagtgcca cctgtcggac gcgtacgcgc
       61 tccccgagta cgagcggatc gtctccgagt acgcccgcag ccatcccgac gttgagtgga
      121 tcctgggcgg ggggtggtcc atggacgtgt tccccggggg gacgccgacg gccgacgcgc
      181 tggaccggct ggtgcccgac cgtcccgtgt acctgccgaa ccgcgatggg cacagcgcgt
      241 gggtgaactc caaggcgctg gagctggccg ggatcaccgc cgagacgtcg gatcctcccg
      301 acggccgcgt cgagcgtggc ctcgacggcg cacccaccgg gaccctgcac gagggagcga
      361 tggatctggt cgagaagctg atccccgaga ccaccgaagc ggagtgggag gaggggctcc
      421 gcgtggcgca gcggtacctg cattccctgg gcatcaccgc ctggcaggac gcgatcgtgg
      481 ggggctcctt cgacacgttc gacgcctacc tggcctgcgc cggccggggg gagctcaccg
      541 ctcgggtggt gggctcgctg tggtgggacc gccatcgtgg ccaggagcag atcgaggaga
      601 tgatcgatcg gcgggcccgt ggcgacgtgg ggcggttcag ggccacctcg gtgaagatca
      661 tgcaggacgg cgtggtggag acgttctccg cggcggtgct cgatccctac ctcgaccggg
      721 acggccgccc gaccgggaac cgggggatct cgttcgtgga ccccgaggcc ctgaagggct
      781 acgtcacccg cctggacgcc gagggcttcc aggtccattt ccatgctctg gcggagcgcg
      841 ccgtgcgcga ggcgctcgac gcgatcgagg ccgcccggcg ttcgaacggg ccgagcgatg
      901 gacgccatca cctggcccac atccaggtcg tccaccccga cgacatcccg cggttcaggc
      961 agctcaacgt ggttgccaac gcccagccgc tgtgggcgat gaacgaggga cagatgcgca
     1021 acctgacgat cccgttcctg ggacccgagc gctccacctg gcagtacccg ttcgcgagcc
     1081 tcgtgcgctc cggggccgtc ctggccatgg gcagcgactg gtcggtgagc agccccaacc
     1141 cgctctggga gatgcacgtg gcggtgaaca ggaccgggtt ccccgggtac gagtacggcg
     1201 atcgggacga cgagctgctc gagccgttcc tgccccgcga gcgcctggac ctgccgacgg
     1261 cgctggcggc cttcacgatg ggctccgcct acgtgaacca cctggaccac ctgacgggct
     1321 cgatcgaggt gggaaagctc gcggacctcg tggtgctcga ccgcaacccg ttcgagcagc
     1381 cgtcggagga gctctacgcg acgaccgtgc agctgaccct ggtggagggg gagcgggtct
     1441 tcgcggaggg ggacctcgcc taaggccggc ggcgcctcgc ccccggggcg gcggacctgg
     1501 ctgccccctg acctgtagtc agggcccctc cttgccaccc gcccggggct cgcgcacgat
     1561 gagccgcgac accgaggatg ccgtggatcg cgcccgcccg catccgtccc ggaagaggag
     1621 gtcccatccg atgacgttcg acatgagcct ggtccagcgt ggggcgcgga accggcgcac
     1681 cccgtactac gaggccaccc agaagcacga tcccaagggg ttcacgatct acaaccacat
     1741 gtacttcccg atccgcttcg acaccttcga ggcggagttc gacgcgctgc tcaccggggt
     1801 gaccctgtgg gacgtctcgg tggagcggtg cctggagatc tccggtcccg acgggttcgc
     1861 gttcgcccag ctgctgacgc cccgggatct gtcccgctgc gccgtcggcc agggcaagta
     1921 cgtgctgatc tgcgactcgg acggggggat cgtcaacgac ccggtcttga ccaggatggc
     1981 ggagaacacc ttctggttcg cgctggcttc cagcgatgcg ctgctgttcg cccgcggcct
     2041 gaagaacgcc tacccccagc tggacgtgac gatccaagag acggacgtgg cccccctgca
     2101 gatccagggc cccaggtcga aggacctgat ggccaagctc gtcggccccg agatcctgga
     2161 ccttcgctac tacttctgga ccgaggcgaa gatcgccggc gctcccgtga tcgtgacgcg
     2221 gacgggctgg acgtcggagg tcggctacga ggtgtacctg acggacacct ccaaggggac
     2281 cgaggtctac gacgccatca tggaggcagg gcagccgttc gggatcaggc ccacggggcc
     2341 cagcgacatc cggcggatcg agggcggcat cttcaactgg ggcgccgata tgacctacga
     2401 gaacaacgcc ttcgagatgg ggctggagcg gctggtcgac ctcgactcgc tcccggacga
     2461 ccggtcgatc tcggtcgccg cctaccgccg gatccgcgag cagggcgtcc gccgcaggat
     2521 caatggggtc gagctcgacg gagagccctt ccccggcctg aacgacatca agtggccggt
     2581 ggtcgagggg gacgcgagga tcgggaaggt gacctcggcc atctactcac cgcgcctgga
     2641 gaagaacatc ggctatgcct ggctgccggc ggaccggtcc tcggccggca cgcgggcatc
     2701 ggtccggacg cagtggggca cccgtaccgc aacggtcgtc cccatgccgt tcgtggaccc
     2761 gagcaagcag atccccgtct cgtagcgttg gcgacgggcg ggcctcgagg ctgaagtgcg
     2821 gttcaggacg gtgtggtgca agaagcaccg tccctgcctt gcgcatggcc ggcggtttga
     2881 acgatgattc aggaaagccg ggccaccggt cgggcgcgag gcgggaggag gatgcgcgca
     2941 tggtgaacga gcgccgggag atgctggaga agagcgccac gatctacttc ggcccctggt
     3001 accggcggtc gcccttcttc gacgccaccc ggcgcgcggg gtgcaccgcg tacgacgtgt
     3061 acaaccacat gctgctgccc gcgtacttcg acgacccgga gaccgagtac tgggccttgc
     3121 tgaacgacgt caccgtgtgg gacgtggcgg tggagcgcac cgttcaggtc tcgggaccgg
     3181 acgcggaccg actgatggac ctgctgacgt gccgggacct cacccggtgc gcggtgaagc
     3241 agggcaagta catgatcgtg acggcgcccg atggggggat cgtgaacgat ccggtcctgc
     3301 tccacgtggg ggagaaccgg tggtggatgc agtgcgccga ctccgacgcc ggtctctacg
     3361 ccctgggcgt ggcctcccgg tcgggactcg acgtggaggt gagctatccc gacgcgtacc
     3421 ccatgcaggt ccagggggcc cgggcgggca agaccctgga gaagctggtc ggcgccgccg
     3481 tg
//
LOCUS       DASQQQ010000245         5751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_265294, whole genome shotgun sequence.
ACCESSION   DASQQQ010000245 DASQQQ010000000
VERSION     DASQQQ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265294"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VE646_09310"
     CDS             <1..266
                     /locus_tag="VE646_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80222.1"
                     /translation="GNHPKAGNKETVSTSLTESGTFPVRNGQTTGTISAGPPGPGDFS
                     CPPGQTLYLMSVTYSGITVTGSAGDYLGATPDPISSGPIMIKV"
     gene            513..1379
                     /locus_tag="VE646_09315"
     CDS             513..1379
                     /locus_tag="VE646_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HZD80223.1"
                     /translation="MLPARFEYHRPDTLDEAVGLLGSLEEPKVLAGGQSLIPLLKLRF
                     AAPSDLVDINRIPGLDRIGEEGGMLRLGALVRHNQVASSDVVAARHPTLAAAAPQIAD
                     PIVRNLGTMAGSLAHADPAGDWGAVMLAVGGTFVLRGPGGEREVAAEDFFQDIFTTVL
                     EPDEILAEVRVPAPTGPVGGTYLKLERKVGDFGTVGVAVHVQMDNGGIGRAGIGLTAV
                     GSRNLRAAEAEAILTGREPGDEVFAEAGRQAAAITSPIADLRGSAEYKRHMVEVYVRR
                     GLARAVEMARAA"
     gene            1392..1883
                     /locus_tag="VE646_09320"
     CDS             1392..1883
                     /locus_tag="VE646_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZD80224.1"
                     /translation="MQITVTVNGVERTADVEPRLLLVHFIRETLGLTGTHIGCDTTSC
                     GACTVLLDGTPVKSCTLFAVMADGRDLSTVEGLQADGALDPIQVAFKEEHGLQCGFCT
                     PAMMLTGKALLEANPDPTEDDIRWAISGQICRCTGYVNIVKAVQRAAQMRSEAPAEAP
                     ATA"
     gene            1899..4247
                     /locus_tag="VE646_09325"
     CDS             1899..4247
                     /locus_tag="VE646_09325"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HZD80225.1"
                     /translation="MATEVEEIRGLGHNVRRKEDDRFIRGRGNYVEDIKLPGMLYMAI
                     LRSPYAHATIKGVDTAAASAAPGVVAVVTGELMAQHNLAWMPTLSYDTQAVLATDKVR
                     FQGQEVAAIIAEDPYQAKDALELIEVDYEPLEPIVTPQRALEADAPVIRTDKEDQKDN
                     HIYHWETGDQEATTEAFDQADLVVKLDTFYPRSHPSPLETCGCVADVDPATGQATIYM
                     TSQAPHAIRTVFAMVAGLPEENIRIVSPDIGGGFGNKVPVYPGYVVATAASLLLGHPV
                     KWMEERTENLISTGFARDFHMHGELALKQDGTMLGLRATILADEGAFHADAQPSKFKV
                     GLFHIVTGSYQLPAAHVVADGAYTNKAPGGVAYRCSFRVTEASYLIERLVQNAAYELE
                     MDPADLRKKNFIREDQFPYTSATGFVYDSGDYHRAMDLALETLAYEDLRREQAEARER
                     GELVGIGLASFTEIVGAGPHKDYDILGLKMNDGAELRIHPTGTAILKISVQTQGQGHE
                     TTFAQIVAEEIGIQPEDVKVMHGDTDNTPYGLGTYASRSTPVSGAATAMVTRRVREKA
                     QKIAAHLLEVSEEDVQFAKGAFSVKGAPDKVTTIQDVALAAYTNFPEGMEPGLEAVYY
                     YDPPNLTFPFGTYAVVVDVDPDTGVWKVRRMVAVDDCGVRINPMIVQGQIHGGLTEGF
                     GIAAMEQIAFDENGNCLGANFTDYLLPTAWETPRFETLETVTPSPHHPIGAKGVGESA
                     TVGSPAAYVNAVIDALWPLGVRNIDMPLTSAKVWEAIQQARS"
     gene            4257..5231
                     /locus_tag="VE646_09330"
     CDS             4257..5231
                     /locus_tag="VE646_09330"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HZD80226.1"
                     /translation="MRADVLELAADLSRRGEPFVLASVVWRRAPSSGKVGSTALVTTD
                     GRVQGWLGGACAEPTVVREALKALEEGTPRLLFLGTPDELQTMQRPDVVSVPIACQSE
                     GALEVYVEPVLPQPSLIVIGRSPAVAALAALGSALGWPTVVVDEGGRPEDYPGAGRVV
                     ASLDLQAAGVNQRSFVVVATQGHYDEDALQRALETPAAYVGLVASAKRAEAVLGYLED
                     RGVSEDALARVHAPAGLDLGRIAHDEIAVAILAELVQLRAAGALEGTVQPAVPERHEE
                     IDPVCHMTVDVASARYRTVHDRRTYYFCSAGCQRRFEEDPAAFAAATT"
     gene            5228..>5751
                     /locus_tag="VE646_09335"
     CDS             5228..>5751
                     /locus_tag="VE646_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HZD80227.1"
                     /translation="MSEISDVLSAIESLSARGEKLALATIVAVRGSTYRRPGARLLVP
                     EEGEVIGNISGGCLENDVAEVGRIVMHEGMARIVSFDLTADDEAVWGWGLGCNGALEL
                     FVEPAEKAAEVAGALRMALEEERPISMVTVLDSSIQAVEQGNRLLVRPDGSVEGSLGD
                     ATADAAAVEAAVDLL"
BASE COUNT          903 a         1969 c         2075 g          804 t
ORIGIN      
        1 ggggcaacca ccccaaggcc ggcaacaagg agacggtctc cacctcgctg acggagtcgg
       61 gcaccttccc ggtccgaaac ggccagacga ccgggacgat ctccgccggg cctccggggc
      121 cgggagactt ctcctgcccg cccggccaga cgctgtactt gatgtccgtg acgtactcgg
      181 gcatcaccgt gaccgggtcg gccggtgact acctcggagc cactccagac cccatctcga
      241 gtggtcccat catgatcaag gtctagtgcg ctagaccgat gcaccaagga ccgggagggc
      301 gctcgcaatc cctcccggtc cgcctccctc tggcttgacc gaaccagacc ggtcggggtc
      361 aaatgaccgg taccgctcac ggtgggcgac gcaggtgggg gtacgtgagt ctgatcggaa
      421 cgcacaagcc caaccttcgc ttccgacctg ctgcccaccg cgctccgcga tctccgttcc
      481 ccgctcgtcc gtatggcctc agggaggtcc gcatgcttcc agctcggttc gagtatcacc
      541 gccccgatac gctcgatgag gccgtcggcc tgctcgggtc gctggaggag cccaaggtct
      601 tggccggggg ccagagcctg atccccctgc tcaagctccg gttcgccgca ccgtcggatc
      661 tcgtcgacat caaccgcatc ccggggctgg accgcatcgg cgaggaaggc ggcatgctgc
      721 ggctcggagc cctggttcgg cacaaccagg tggcctcctc cgacgttgtg gcggcccgcc
      781 accccacgct ggcggccgcg gctccgcaga tcgccgatcc gatcgtgcgg aacctgggga
      841 cgatggccgg gtcgctggcc cacgcggatc ccgccggtga ctggggcgcc gtgatgctgg
      901 ccgtcggcgg cacgttcgtg ctgcggggcc cgggtggcga gcgggaggtg gccgccgagg
      961 acttcttcca ggacatcttc accaccgtcc tggagcccga cgagatcctc gcggaggtcc
     1021 gggtcccggc gcccaccggc ccggtgggcg gcacctacct gaagctcgag cgcaaggtgg
     1081 gcgacttcgg caccgtggga gtggccgtcc acgtgcagat ggataacggc gggatcggcc
     1141 gggccgggat cgggctcacg gcggtgggct cgaggaacct ccgggccgcc gaggccgaag
     1201 ccatcctcac cggccgggag cccggcgacg aggtgttcgc ggaggcgggt cggcaggcgg
     1261 cggccatcac gagcccgatc gcggatctcc ggggctcggc cgagtacaag cgccacatgg
     1321 tcgaggtcta cgtccgccgc gggctggccc gcgccgtcga gatggcccgg gccgcctgag
     1381 gaggaggaac gatgcagatc acggtgaccg tcaacggggt ggagcggacg gccgacgtgg
     1441 agccgcggct cctcctcgtc cacttcatcc gcgagacgtt gggactgacc ggcacccaca
     1501 tcgggtgcga caccaccagc tgcggtgcct gcaccgtgct cctggacggg accccggtga
     1561 agtcctgcac cctgttcgcg gtcatggccg atgggcggga cctctccacg gtagagggcc
     1621 tgcaggccga cggggccctg gatccgatcc aggtggcgtt caaggaggag cacggcctgc
     1681 agtgcgggtt ctgcaccccc gccatgatgc tgaccggcaa ggcgctgctg gaggccaacc
     1741 cggaccccac cgaggacgat atccggtggg ccatctccgg gcagatctgc cggtgcaccg
     1801 gctacgtgaa catcgtcaag gcggtccagc gggcggcgca gatgcgctcg gaggcgccgg
     1861 cggaagcccc tgccacggcc tgaggaggaa acgaccacat ggcgaccgaa gtggaagaga
     1921 tccgcgggct gggacacaac gtccgacgca aggaggacga ccggttcatc cgcggccggg
     1981 gcaactacgt cgaggacatc aagctccccg gcatgctgta tatggcgatc ctgcgcagcc
     2041 cgtacgcgca cgcgacgatc aagggcgtcg acaccgccgc ggcctccgcg gcccccgggg
     2101 tggtggccgt ggtgacgggc gagctcatgg cccagcacaa cctggcgtgg atgcccaccc
     2161 tgtcctacga cacccaggcg gtgctggcca cggacaaggt gcgcttccag gggcaggagg
     2221 tggcggcgat cattgccgag gatccgtacc aggccaagga cgcgctcgag ctgatcgagg
     2281 tcgactacga gccgctggag ccgatcgtca cgccccagcg ggcgctggag gccgacgcgc
     2341 cggtgatccg caccgacaag gaggaccaga aggacaacca catctaccac tgggagacgg
     2401 gtgaccagga ggccacgacc gaggcgttcg accaggcgga cctggtggtg aagctcgaca
     2461 ccttctaccc gcgctcgcac ccctctccgc tggagacctg cggatgcgta gccgacgtcg
     2521 acccggccac gggtcaggcc acgatctaca tgacctcgca ggccccgcac gcgatccgca
     2581 cggtcttcgc gatggtggcg ggcctgccgg aggagaacat ccggatcgtc tctcccgaca
     2641 tcggcggggg gttcgggaac aaggttccgg tctaccccgg ctacgtggtg gccacggccg
     2701 cctccctgct cctcggtcac cccgtgaagt ggatggagga gcgcaccgag aacctgatct
     2761 ccaccgggtt cgcccgggac ttccacatgc acggcgagct ggccctgaag caggacggga
     2821 cgatgctcgg gctgcgggcc acgatcctgg ccgacgaagg cgcgttccac gccgacgccc
     2881 agccgtccaa gttcaaggtg gggctgttcc acatcgtgac cggctcctac cagctgccgg
     2941 ccgcccacgt ggtggccgac ggcgcctaca cgaacaaggc gcccggaggc gtggcctacc
     3001 ggtgctcgtt ccgggtgacc gaggcctcgt acctgatcga gcgcctggtc cagaacgcgg
     3061 cctacgagct cgagatggac ccggcggacc tgcggaagaa gaacttcatc cgcgaggacc
     3121 agttcccgta cacctcggcc accgggttcg tctacgactc cggcgactac caccgggcga
     3181 tggacctggc cctggagacc ctggcgtacg aggacctgcg cagggagcag gccgaggcgc
     3241 gcgagcgcgg cgagctcgtg ggcatcggcc tggccagctt caccgagatc gtgggcgccg
     3301 gcccgcacaa ggactacgac atcctcgggc tgaagatgaa cgacggtgcc gagctgcgga
     3361 tccaccccac gggcaccgcg atcctgaaga tcagcgtcca gacccagggg caggggcacg
     3421 agaccacgtt cgcgcagatc gtggccgagg agatcgggat ccagcccgag gacgtcaagg
     3481 tgatgcacgg cgacacggac aacaccccct acggcctggg cacgtacgcg tcgcgatcca
     3541 ccccggtctc gggcgcggcc acggcgatgg tgacccggcg ggtccgggag aaggcgcaga
     3601 agatcgccgc ccacctgctc gaggtctccg aggaggacgt gcagttcgcg aagggggcgt
     3661 tctcggtcaa gggcgcgccg gacaaggtca ccacgatcca ggacgtggcc ctcgccgcct
     3721 acacgaactt ccccgagggg atggagccgg gcctggaggc cgtctactac tacgacccgc
     3781 cgaacctcac gttcccgttc ggcacctacg cggtggtggt cgacgtcgat cccgacaccg
     3841 gtgtgtggaa ggtccgccgg atggtcgcgg tggacgactg cggggtccgg atcaacccga
     3901 tgatcgtcca ggggcagatc cacggcggcc tcaccgaggg gttcggcatc gcggcgatgg
     3961 agcagatcgc cttcgacgag aacggcaact gcctgggtgc gaacttcact gactacctcc
     4021 tgcccacggc ctgggagacg ccgcggttcg agacgctcga gaccgtgacg ccctcgccgc
     4081 accatccgat cggcgccaag ggcgtcggcg agtcggccac cgtggggtcc cccgccgcct
     4141 acgtcaacgc cgtgatcgac gcgctctggc cgctcggcgt gcgcaacatc gacatgccgc
     4201 tcacttccgc caaggtgtgg gaggccatcc agcaggcccg gtcgtagcct ggacgtatga
     4261 gggccgacgt cctcgagctc gcggcggacc tgtcccggcg gggtgagccg ttcgtgctgg
     4321 ccagcgtggt gtggcgacgg gcgccctcct cgggcaaggt cggctccacg gccctggtga
     4381 ccacggatgg acgggtgcag ggttggctgg gcggggcgtg cgccgagccc acggtggtgc
     4441 gcgaggcgct caaggcgctg gaggagggca cgccccgact gctgttcctc ggcacccccg
     4501 acgagctgca gacgatgcag cgccccgacg tcgtgtccgt gccgatcgca tgccagagcg
     4561 agggagccct ggaggtgtac gtggaacccg tcctgccgca gccgtcgctg atcgtgatcg
     4621 ggcggtctcc cgcggtggcc gcgctggcgg ccctgggatc ggccctgggc tggccgacgg
     4681 tcgtggtgga cgaggggggc cggccggagg actatcccgg tgcgggacgc gtggtcgcct
     4741 cgctggacct gcaggcggcc ggggtgaacc aacgctcgtt cgtcgtggtg gccacgcagg
     4801 gccactacga cgaggacgcg ctccagcgcg ccctcgagac gccggcggcg tacgtcgggc
     4861 tggtcgcgtc cgccaagcgc gcggaggccg tgctggggta cctggaggac cgcggcgtgt
     4921 cggaggatgc gctggcgagg gtccacgcgc cggccgggct cgacctcggc cgcatcgccc
     4981 acgacgagat cgccgtggcc atccttgcgg agctcgtgca gctgcgcgcc gcgggagcgc
     5041 tggaggggac ggtccagccc gccgtgcccg agcggcacga ggagatcgat cccgtctgcc
     5101 acatgaccgt cgacgtcgcc tcggcccggt accggaccgt gcacgatcgg cggacgtact
     5161 acttctgctc ggcgggctgc cagaggcggt tcgaggagga tccggccgcg ttcgcggccg
     5221 ccacgacatg agcgagatca gcgacgtcct ttcggccatc gagtcgctct ccgcgcgggg
     5281 ggagaagctc gccctggcca cgatcgtggc ggtgaggggc tcgacctatc ggcgccccgg
     5341 cgcccgcctg ctggtgcccg aggaaggcga ggtgatcggc aacatcagcg gcgggtgcct
     5401 ggagaacgac gtggccgagg tgggacgcat cgtgatgcac gagggcatgg cccggatcgt
     5461 ctccttcgac ctgaccgccg acgacgaggc cgtctggggc tggggtctgg gctgcaacgg
     5521 ggccctggag ctgttcgtgg agcctgccga gaaggccgcg gaggtggccg gtgccctccg
     5581 gatggccctg gaggaggagc gccccatctc gatggtcacg gtcctggact cctccatcca
     5641 ggccgtggag caggggaacc ggctgctggt ccggcccgac ggatcggtgg agggatccct
     5701 cggggatgcc accgcggatg ccgcagcagt ggaggccgcc gtcgacctgc t
//
LOCUS       DASQQQ010000246         9625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_185845, whole genome shotgun sequence.
ACCESSION   DASQQQ010000246 DASQQQ010000000
VERSION     DASQQQ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9625
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185845"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VE646_09340"
     CDS             <1..604
                     /locus_tag="VE646_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZD80228.1"
                     /translation="GLAERAAAQVTELMSLLPGHPPWGAQGDAARTRVALARGANEEA
                     LAAAHSAFGAMLAAMQEDLHLDVLLPVAEAVLAAGTDEERSMVGSQLRTVLALIGQRT
                     LDGEVRARWFHGPVGGPLSGLLGVAPQPADGDGAGSSLAEGQVELLHLVIEGRTNAEI
                     ARSLGIDEPEVTRRLGELFASIGASSRAEATAVAFREAMV"
     gene            607..1338
                     /locus_tag="VE646_09345"
     CDS             607..1338
                     /locus_tag="VE646_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZD80229.1"
                     /translation="MIRIRVDRHRCVGAGNCITIAPTAFDWYEGDLLKADVLDAESVE
                     EELLREAALACPTQAIVIDEVEQLLPWQMRGSTAPRRVEKTFMFTDIVKSTNLIEVLG
                     DEAWEALQHWHDETLRSIFAAHQGEEVSALGDGFFVSFDSPDEALACAVAIERRLAEQ
                     RQAQGFAPQVRIGVHATGATQVGRNFTGRGVHEAARIGALADGGEILASRSTAAGSRF
                     PASEPRTVSLRGVSEPVEIVSVEWR"
     gene            1457..1843
                     /locus_tag="VE646_09350"
     CDS             1457..1843
                     /locus_tag="VE646_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80230.1"
                     /translation="MVSMWRWLFTGFLVAHGVIHLALWVPSQTEQGRIPGHSWLLGDQ
                     RSIAVAITIVATALFVAAGAGLLFHAGWWRPLTVLAGIVSISLVIVFPEPFRPYAWVL
                     SPLAINGALIAAILWFSWPSERMVGA"
     gene            complement(1857..2423)
                     /locus_tag="VE646_09355"
     CDS             complement(1857..2423)
                     /locus_tag="VE646_09355"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZD80231.1"
                     /translation="MRGFELGAAIITERLRLRPFTADDLPALLAMQSDPEVTRYLYWD
                     VRSEPQVRTALEKKIAATAIHADGDVLAAAAELLATGELVADLVLHLVSGEHRTAEIG
                     FIVPPAHQRRGYATEAARAVLRIAFDELGLHRVIGRLEARNTASARVLEKLRMRREAH
                     LVENEFVKGEWQSELIYAILDREWHQAP"
     gene            2565..2963
                     /locus_tag="VE646_09360"
     CDS             2565..2963
                     /locus_tag="VE646_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80232.1"
                     /translation="MRLTWRDGVATVFMVLVALVTMAVSGSWGWPLLGGYRTGALALF
                     VLAQPICRTGGAAFWTSEAIKRPALVLHDPFILVAMVLGVGATVLVVLGLVLGTRAPF
                     LALAGVMGVLWLIATIRHVAEAAPRLHPAS"
     gene            3056..3946
                     /locus_tag="VE646_09365"
     CDS             3056..3946
                     /locus_tag="VE646_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZD80233.1"
                     /translation="MLQIQDLTKRYGDVLALAGATFSVRPGRIVGFLGPNGAGKTTTM
                     RAIFGLVRPDAGRVLWDGHPVGPRDRDRFGYMPEERGLYSKMRVEEQLAYFAELSGLD
                     ARRAKEAATRWLDRLGLADRAAARLEELSHGNQQRVQLAAALVHAPELIVLDEPFGGL
                     DPLGVDALAQMLVETAAQGVGVLFSSHQLDLVEDVCQDVVIIDHGRIVLAGAVEELKA
                     ASSQRSLVVTVDGEPWIPDLSGATVTNRQDGQVRVLVDESVDLDAVLRQAEAAGAVTR
                     FSFEPPSLTDLFRRAVGRQS"
     gene            3943..5127
                     /locus_tag="VE646_09370"
     CDS             3943..5127
                     /locus_tag="VE646_09370"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD80234.1"
                     /translation="MRGFRRAWLVARREWNVRVRSRAFRISTLVAVAIVVAIIVVPAT
                     VGGAGTPTRTVGLVGPSSPQLPELLGQAGRPFGVRVETRAFDSLASAQTALRSGDVDV
                     VLEDERTTVWSSAPDDQLQAIVRSAVQGAHQQRAIEQIGLSPAEVRQLLAPPQLRSRS
                     LEPATKERSARITLATVGLVLLFMAINFYGGFLLVGVIEEKSSRVVEVLLSRVRPKEL
                     LAGKITGIGLVGLAQFVIVAVSALLALAVTGNAEAPTTAPGTIGWILVWFVLGYGFYS
                     VLYASAGSLVSRQEEAQSMTFPIAGVLLVGYVLSFGVVHSPHGGVALVGSFVPPTAPM
                     IMIVRIASGGVAWWQVAASIVLTMASIYGMVLVAARIYAGAILRIGRRVRLGEAWRAA
                     AP"
     gene            5170..6666
                     /locus_tag="VE646_09375"
     CDS             5170..6666
                     /locus_tag="VE646_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZD80235.1"
                     /translation="MAEVQTLLHDRYEIRSLLGRGGLARVFLGRDVLEGRQVAVKILA
                     APFDRDRAVAERFCEEARGAARLEHPNIVSVLDVGSQDGLHFLVMEHVQGRTLTEVLA
                     EEGPLPPERVAEIGAEVALALGAAHTHGVIHGDLTPGNVMQIGGAGVKVSDFAIARAA
                     DARSAARSGFIMGTARYLAPERARGPADPRSDVYSLGCVLHELVTGRPPFSAESAVGT
                     LFQHVYEPLTPPSRIRSMPAGLEAIILRCLAKDPEQRYADASGLSADLRRSWSSPVPP
                     APPRRGSRASTTGPRTQIQGARRSKRRLRRPRDGAGPGPGGPTSAAGPHRPRRPALAP
                     VLALVAAAAAIVVIIGGMGLGTVHWSALPPSSVSPVDGSGATAGPTSAAAGAGAPSIT
                     EPTPAVPDAYSSFVDTIGMGRGAGMIDGRGSTALIQGARDAYSAYQQGRVRTALTRMA
                     DLNALRRSLLSQGHITEAAASSVQRAITTLTQAMRSAAMSSPAAGGPG"
     gene            complement(6663..7349)
                     /locus_tag="VE646_09380"
     CDS             complement(6663..7349)
                     /locus_tag="VE646_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZD80236.1"
                     /translation="MSRTVTLPRLALLAGAAAAYATVLRPRQLRWGATVDEDLEELPG
                     DDLLPNARSQATRAITIEARPSDVWPWLVQIGADRGGFYTYDALENAFGLGIRSVERL
                     VSGWQHLRPGDFVAADRRCRGGWFVERLEPERCLVLWTGDPRTGGRFDGRDTPAGYTW
                     TYVLRERADGGTRLICRARFAYRSPLLLAPVEVIELVDWFMTQRMLRGIRDRAERCAP
                     TPVSLEGTAA"
     gene            complement(7474..8841)
                     /locus_tag="VE646_09385"
     CDS             complement(7474..8841)
                     /locus_tag="VE646_09385"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DedA family/phosphatase PAP2 family
                     protein"
                     /protein_id="HZD80237.1"
                     /translation="MIASIVDRILSLHGFTALLVVFLLPALEASAFVGFVFPGEVAAL
                     LAGVLAFQGRISLWAAIVAVVLGAVAGDSVGYAIGRRWGRRILTGVIGRVPFLGHRVE
                     EHLDSAEAFLRRRRGSAVFFGRFTAALRVMVPGLAGMSRIPFPVFAAYNVAGGVLWGA
                     GFVLLGFSAGAAWRSVEGVATRVGLALLVAIVGGLIAVRLVRRWRRAEAAERGPLSRL
                     RRRFPRTGDWVAARIDPTTATGFPLTLTVLLAGASAWTFVGLTQDVLTNEESVRWDPT
                     VASWFVHHRVGWATAGMKVVTFLGSNVVLIPLGVLVAVSLWVVWRNWRPGVLLLSSMG
                     GAILLYQGFKAFVDRPRPRASIHLVSAGGSAFPSGHATQVVASFATLALLLSVGRPWR
                     MLAPTWTLAVIVVLAVGASRLYLGVHWLTDVLGGYTLGGAWVGILWSTVLGLHVPLGP
                     RRRPP"
     gene            complement(8846..>9625)
                     /locus_tag="VE646_09390"
     CDS             complement(8846..>9625)
                     /locus_tag="VE646_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZD80238.1"
                     /translation="IERSFPSVPGHVWELVRRSNPETISPTGRWQLHVHAFLLRANGR
                     TLLFDAGFGGGDAPAVAWTGTGGRLMEELAALSVDPSEVELVVLSHAHDDHVGWAVGA
                     DGGPAFPKARYLLHEADWGWVRRPDDPDNGPVAERCLRPLAAAGILDTCGDGHRIGTG
                     LTLRHAPGHTPGHQVLVIDSAGARGMLAADSVNHPVQLVDPTWFGTSDDDPRLAAATR
                     AALVDLAERQELLVVTSHFPNPFGRIVRHEGRHAWSPVRGT"
BASE COUNT         1291 a         3361 c         3611 g         1362 t
ORIGIN      
        1 gggcctcgcg gagcgagcgg ccgcgcaggt caccgagctg atgagcctgc tccccgggca
       61 ccctccctgg ggggcgcagg gggatgccgc tcggacccgg gtcgccctgg cccgaggggc
      121 gaacgaggag gccctggcgg cagcccactc ggcgttcggg gcgatgctgg cggcgatgca
      181 ggaggacctg cacctggacg tgctgctccc ggtggcggag gcggtgctgg cggccggcac
      241 cgacgaggag cgctcgatgg tgggttcgca gctgcgcacc gtgctggccc tgatcggtca
      301 gcgcaccctg gacggcgagg tccgggcccg gtggttccac gggccggtgg gcggacccct
      361 gtcgggcctg ctgggggtcg ccccgcagcc ggccgacggc gacggtgcgg gctcctccct
      421 cgccgaaggg caggtggagc tgctgcatct ggtcatcgag gggcggacga acgccgagat
      481 cgcccgctcc ctcggcatcg acgagcccga ggtgacccgg cggctgggcg agctgttcgc
      541 gtcgatcggg gcctcctccc gggccgaggc gacggcggtg gcgttccggg aggcgatggt
      601 ctagccgtga tcaggatccg cgtggatcgc caccgctgtg tgggggcggg caactgcatc
      661 accatcgccc ccaccgcgtt cgactggtac gagggcgacc tgctgaaggc cgacgtgctc
      721 gatgccgaga gcgtggagga ggagctgctg cgggaggcgg ccctggcctg ccccacccag
      781 gccatcgtga tcgacgaggt ggagcagctg ctgccctggc agatgcgggg cagcaccgca
      841 ccgcgccggg tggagaagac cttcatgttc accgacatcg tgaagtcgac gaacctgatc
      901 gaggtccttg gcgacgaggc ctgggaggcg ctgcagcact ggcacgacga gaccctgcgc
      961 tcgatcttcg ccgcgcacca gggcgaggag gtctcggccc tgggcgacgg cttcttcgtg
     1021 agcttcgact ccccggacga ggccctggcc tgcgccgtgg cgatcgagcg ccggctggcg
     1081 gagcagcgcc aggcccaggg cttcgccccc caggtgcgca tcggcgtgca cgccacgggc
     1141 gccacccagg tggggcggaa cttcaccggt cgaggcgtgc acgaggcggc ccggatcggc
     1201 gccctggccg acggtgggga gatcttggct agccgctcca cggcggccgg atcgcgcttc
     1261 ccggcctccg agcctcggac ggtcagcctg cgcggggtct ccgagcccgt cgagatcgtc
     1321 tccgtggagt ggcgttagcc cccgagccgt tcgcgaggga cgggaaggac cttcggaccg
     1381 cgagtctcgg gacgggttcc tcaaggccgg acccgatcgc tcggggcaag gtggggacgg
     1441 acgccgatcg ggaggggtgg tgtcgatgtg gcggtggctc ttcacggggt tcctcgtggc
     1501 tcacggggtg atccacctgg cgctgtgggt cccgtcccag acggagcagg gacgcatccc
     1561 ggggcactcg tggctgttgg gcgaccaacg gtcgatcgcc gtggcgatca cgatcgtggc
     1621 cacggcgctg ttcgtggcgg cgggggcggg gctcctgttc cacgcgggct ggtggcgtcc
     1681 cctcacggtg ctcgccggga tcgtctcgat ctcgctggtg atcgtgttcc ccgagccgtt
     1741 ccggccctac gcgtgggtgc tctcgcccct ggccatcaac ggcgcgctga tcgcggcgat
     1801 cctgtggttc tcgtggccga gcgagcggat ggtcggggcc tgagccccgg cgatcctcag
     1861 ggagcctggt gccactcccg gtcgaggatc gcgtagatca gctcgctctg ccattcaccc
     1921 ttcacgaact cgttctcgac gaggtgcgcc tcgcgccgca tgcggagctt ctcgagcacc
     1981 cgagccgagg cggtgttccg tgcctccagc cgtccgatca cccgatgcag cccgagctcg
     2041 tcgaaggcga tccgcaggac ggctcgggct gcctccgtgg cgtacccccg acgctggtgg
     2101 gcgggaggca cgatgaagcc gatctccgcc gtccggtgct cgccgctgac caggtgcagc
     2161 acgaggtcgg cgacgagttc gcccgtggcg aggagctcgg cggccgcggc cagcacgtca
     2221 ccgtcggcgt ggatcgcggt ggcggcgatc ttcttctcca gcgcggtgcg gacctggggc
     2281 tcggaccgca cgtcccagta caggtaccgg gtgacctccg ggtcggactg catcgcgagc
     2341 agcgccggga gatcgtcggc ggtgaagggt cgaagccgca gccgctcggt gatgatcgca
     2401 gcacccagct cgaaccctcg caccctgcgc gttcctcccc ggtcccggat ggtccatggt
     2461 agaccgtgcg gtcgctatgg cccttccggg gcaggacctt cgacggtggc tcggctccta
     2521 agcccctggt gatgctggcc ggggcgacct tgagggggtc cgcgatgcgc ctgacctgga
     2581 gggacggagt ggccacggtg ttcatggtcc tcgtggcgct ggtcacgatg gccgtttcgg
     2641 gatcctgggg gtggccgctg ctcggcggct accggaccgg ggccctggcg ctgttcgttc
     2701 ttgcccagcc gatctgccgt acggggggag ctgcattctg gaccagcgag gcgatcaagc
     2761 gtcccgccct cgtcctgcac gaccccttca tcctggtggc gatggtgctg ggcgtggggg
     2821 ccacggtcct cgtggtgctc ggactggtcc tggggacccg agcgccgttc ctggcgctgg
     2881 ccggggtgat gggcgtgctg tggctgatcg ccaccatccg gcacgtcgcc gaggcagctc
     2941 ctcgtctgca cccagcctcg taaggacttg gcgcgcggat cgtatggagc gggccgaagg
     3001 ccctccgtcg gttccgtggc cctccggtat ggtgccggca cgagcggagg accgggtgct
     3061 gcagatccag gacctgacca agcggtacgg cgacgtcctg gccctggccg gggccacgtt
     3121 cagcgtgcga ccggggcgga tcgtggggtt cctggggccg aacggtgccg gcaagaccac
     3181 cacgatgcgg gcgatcttcg gcctggtccg gcccgacgcg ggccgggtgc tgtgggatgg
     3241 acatccggtg gggcccaggg atcgcgatcg gttcgggtac atgcccgagg agcgtggcct
     3301 gtactcgaag atgcgggtgg aggagcagct cgcgtacttc gccgagctgt cgggcctgga
     3361 cgcccgtcgg gccaaggagg cggccacccg atggctggac cgcctggggc tggccgaccg
     3421 cgcggccgcc cggctggagg agctgtccca cggcaaccag cagcgggtcc agctcgccgc
     3481 cgctctggtg cacgcccccg agctgatcgt gttggacgag ccgttcggcg ggctcgaccc
     3541 cctgggcgtg gacgccctgg cgcagatgct ggtggagacc gcggcccagg gcgtgggggt
     3601 gctgttctcg agccaccaac tcgacctggt ggaggacgtc tgtcaggacg tggtgatcat
     3661 cgatcacggg aggatcgtgc tggccggggc ggtcgaggag ctgaaggcgg cctcgtcgca
     3721 acgctcgctc gtcgtgacgg tcgacggtga accctggatc ccggacctgt ccggcgcgac
     3781 cgtgacgaac cggcaggacg gccaggtccg cgtgctggtg gacgagtccg tggacctgga
     3841 cgccgtgctc cgccaggcgg aggcggccgg agcggtcacc cggttcagct tcgagccgcc
     3901 gagcctcacg gacctgttcc gccgagcggt ggggaggcag tcgtgagggg gttccggcgc
     3961 gcctggctgg tcgctcgccg cgagtggaac gtccgggtcc gctcgcgcgc gttccgcatc
     4021 tccacgctgg tagccgtcgc gatcgtggtg gctatcatcg tcgtgccggc cacggtgggg
     4081 ggcgccggca cccccacccg caccgtgggg ctggtcgggc cgagctcgcc gcagctgccc
     4141 gagctgctcg ggcaggcggg gcggcccttc ggcgtccggg tcgaaacgcg tgccttcgac
     4201 tccctggcgt ccgcgcagac cgcgctgcgc tccggcgacg tggacgtggt cctggaggac
     4261 gagcggacca cggtctggtc gtccgcgccc gacgaccagc tccaggccat cgtccggtcg
     4321 gccgtgcagg gggcgcacca gcaacgggcg atcgagcaga tcggcctgtc gcccgccgag
     4381 gtgcggcagc tcctggctcc tccacagctc cgctcgcgga gccttgaacc cgccacgaag
     4441 gagcggtccg cccgcatcac gctggccacg gtcgggttgg tgctcctgtt catggcgatc
     4501 aacttctacg gtggtttcct cctggtgggc gtgatcgagg agaaatccag ccgggtggtg
     4561 gaggtcctgc tgtcccgtgt gcggccgaag gaactgctgg cgggcaagat caccgggatc
     4621 gggctggtgg ggctggcgca gttcgtgatc gtggcggtct ccgccttgct ggcgctggcg
     4681 gtgaccggca acgccgaggc gccgaccacc gcgccaggca cgatcgggtg gatcctcgtc
     4741 tggttcgtcc tgggctacgg gttctactcg gtcctgtacg cgtcggcggg gtcgctggtc
     4801 tcccgccagg aggaggcgca gagcatgacc ttccccatcg ccggtgtgct gctggtgggg
     4861 tacgtcctct cgttcggggt ggtccattcc ccccacggcg gggtggcgct ggtggggtcg
     4921 ttcgtgcccc ctacggctcc catgatcatg atcgtgcgga tcgccagcgg gggggttgcc
     4981 tggtggcagg tcgccgcctc gatcgtgctg accatggcca gcatctacgg gatggtgctg
     5041 gtggccgcca ggatctacgc cggagcgatc ctgcgcatcg gccgacgggt gcggttgggc
     5101 gaagcctggc gggccgccgc accgtagggc cgggtgccgg gcacgtcggc tgggtatgtc
     5161 gagcctgtca tggccgaggt ccagaccctg ctccacgatc gctacgagat ccgctccctg
     5221 ctggggcggg gcggtctggc ccgggtgttc ctgggacggg acgtgctcga ggggcgccag
     5281 gtggccgtca agatcctggc cgcccccttc gaccgggatc gggcggtcgc ggagcgcttc
     5341 tgcgaggagg cccggggggc ggcgcggctg gagcatccga acatcgtctc ggtcctcgat
     5401 gtcggctccc aggacggcct gcacttcctg gtgatggagc atgtgcaggg gcgcacgctc
     5461 accgaggtgc tggcggagga gggcccgctg ccccccgagc gggtggccga gatcggggcg
     5521 gaggtggccc tggcgctcgg ggcggcgcac acccacggtg tgatccacgg cgacctcacg
     5581 ccgggcaacg tcatgcagat cgggggcgcc ggggtcaagg tgtcggactt cgccatcgcc
     5641 cgagccgccg atgcccgttc ggccgcccgc agcgggttca tcatgggcac cgcccggtac
     5701 ctcgcccccg aacgcgcccg gggcccggcc gacccccgct ccgacgtgta ctcgctcggg
     5761 tgcgtgttgc acgagctggt cacgggtcgt ccgccgttct cggccgagtc ggccgtgggc
     5821 acgctgttcc agcacgtgta cgagccgctc accccgccct ccaggatccg gtcgatgccg
     5881 gccggactcg aggcgatcat cctgcggtgc ctggccaagg atcccgaaca gcgatacgca
     5941 gacgcgtcgg gactgtccgc ggacctgcga cggtcgtggt cgtcgccggt cccccccgcc
     6001 ccgccgaggc gtggttcgag ggcgtcgacg accgggcccc ggacccagat ccagggagcc
     6061 cgacgctcga agcgccggct ccgacgaccc cgagacgggg cagggccggg accgggcggt
     6121 ccgacatcgg cggccggtcc gcaccgcccc cggcgaccgg cgctggcgcc ggtcctggcg
     6181 ctcgtggcgg cggccgcagc gatcgtggtc atcatcggag ggatgggcct cggcaccgtt
     6241 cactggtccg cccttccgcc gtcgtcggtc tcgccggtgg acggctccgg tgccaccgcc
     6301 ggtcccacga gcgcggccgc cggcgccggg gccccgtcga tcaccgagcc cacgccggcg
     6361 gtccccgatg cgtactcctc gttcgtggac acgatcggca tgggccgcgg agcgggcatg
     6421 atcgacggac gagggagcac ggccctgatc cagggagccc gggacgccta cagcgcctac
     6481 cagcagggcc gggtgcggac ggcgctgacg cggatggcgg atctgaacgc cctgcggcga
     6541 tcgctgctga gccaagggca catcaccgag gccgcagcgt cctccgtgca gcgagccatc
     6601 accaccctga cgcaggcgat gcgctcagct gcgatgtcca gtccggccgc aggcggaccg
     6661 ggttaggcgg cggtgccctc caggctgacc ggggtgggcg cgcaacgctc ggcccggtcc
     6721 cggatcccgc ggagcatccg ctgggtcatg aaccagtcga ccagctcgat cacctcgacc
     6781 ggcgccagga gcagggggct ccggtaggcg aatcgggccc ggcagatgag ccgggtgccg
     6841 ccgtcggccc gttcacgcag gacgtatgtc caggtgtacc cggccggggt gtcgcgtcca
     6901 tcgaaccggc cgccggtcct gggatcgccc gtccacagca ccaggcaccg ctcgggctcg
     6961 agccgctcga cgaaccagcc gccccgacac cggcggtcgg cggccacgaa gtctcccggc
     7021 cgcaggtgct gccagccgct caccaggcgc tcgacgctcc ggatccctag accgaacgcg
     7081 ttctccagcg cgtcgtacgt gtagaagccg ccacggtcgg ctccgatctg gaccagccac
     7141 ggccacacgt cggagggacg cgcctcgatc gtgatggctc gggtggcctg gctgcgggcg
     7201 ttcggaagga ggtcgtcgcc gggcagctcc tccaggtcct cgtccacggt ggcgccccac
     7261 ctgagttgac ggggccgaag gaccgtggcg tacgcggcgg ccgccccggc caagagcgcg
     7321 agacgtggga gcgtgacggt gcgggacatg atccacctcc ggagtcgttc ggctccacgg
     7381 taggtgccgg acgatgctcg ccggagggcc gctcggccca tgccgttggg gtcgggtggg
     7441 tcgaggctcg gatccgggtc cggatcgacc gggtcagggg ggccgccggc gcggcccgag
     7501 cggcacgtgc aggccgagca cggtgctcca caggatcccc acccatgcgc cgccgagcgt
     7561 gtagccgccc aggacgtccg tgagccagtg cacgcccagg tacaggcggg acgcgcctac
     7621 cgccaggacc acgatcacgg cgagcgtcca cgtcggcgcg agcatccgcc agggccgccc
     7681 gaccgacagc aggagcgcca gcgtggcgaa gctcgcgacg acctgcgtgg catgaccgga
     7741 ggggaacgcg gagccgccgg cggagaccag atggatcgac gcccggggac ggggccggtc
     7801 gacgaacgcc ttgaacccct ggtacagcag gatcgctccg cccatcgaag acagcagcag
     7861 cacgccaggt cgccagttgc gccacacgac ccacaggctc acggcgacga ggacgcccag
     7921 cgggatcagc accacgttgg agccgaggaa ggtgaccacc ttcatgcccg ccgtggccca
     7981 gcccacccga tggtgcacga accaggaggc caccgtggga tcccagcgga cggactcctc
     8041 gttcgtcagc acgtcctggg tgagacccac gaaggtccat gcgctggcgc cggccaacag
     8101 caccgtcagc gtcagcggga accccgtggc ggtggtggga tcgatccgtg cggccaccca
     8161 gtcgcccgtc cgggggaagc gtcggcgcag ccgcgacagc ggcccgcgct cggcagcctc
     8221 ggcccgccgc cacctgcgga cgagccggac cgcgatcagg ccgccgacga tcgcgaccag
     8281 cagggcgagc cccacccggg tggccacccc ctccaccgag cgccaggcgg cgcccgccga
     8341 gaacccgagc agcacgaagc cggcgcccca cagcacgccc ccggccacgt tgtaggcggc
     8401 gaacacgggg aaggggatcc gcgacatccc ggccagtccc ggcaccatca cccgaagcgc
     8461 ggcggtgaac cgtccgaaga agaccgcgct gccccggcgc ctgcgcagga aggcttcggc
     8521 gctgtccagg tgctcctcga cgcggtgccc caggaagggg acccgcccga tcaccccggt
     8581 gaggatccgg cggccccacc gtcgtccgat cgcgtagccg accgaatccc ccgccaccgc
     8641 cccgagcacc acggccacga tggccgccca cagcgagatc cgcccctgga acgcgagcac
     8701 cccggccagg agcgcggcca cctcgccggg gaacacgaac cccacgaacg cggaggcctc
     8761 cagggccggg aggaggaaga cgacgagcag ggcggtgaag ccgtgcaggg acaggatccg
     8821 gtccacgatg ctggcgatca cgggttcagg ttccccggac cgggctccag gcgtgccggc
     8881 cctcgtgccg gacgatccgc ccgaaggggt tggggaagtg cgaggtcacg accaagagct
     8941 cctggcgctc cgccaggtcc accagcgccg ctcgggtggc ggcggcgagg cgcggatcgt
     9001 cgtcggacgt gccgaaccag gtggggtcca ccagctgcac cggatggttg accgagtcgg
     9061 ccgcgagcat cccgcgcgct cccgccgagt cgatcaccag cacctggtgt cccggcgtgt
     9121 gaccgggggc gtgtcgcagc gtcaggcccg tcccgatccg gtgcccgtcc ccgcaggtgt
     9181 cgaggatccc ggccgctgcc agcggacgca ggcatcgctc ggccacgggg ccgttgtccg
     9241 gatcgtcggg acggcggacc cagccccagt ccgcctcgtg cagcaggtac cgggccttcg
     9301 ggaacgcagg ccccccgtcg gccccgacgg cccagccgac gtggtcgtcg tgggcgtggg
     9361 agaggaccac cagttccacc tccgacggat ccaccgacag ggcggcgagc tcctccatca
     9421 gccgtccacc ggtgcccgtc cacgcgaccg cgggggcgtc gcctcccccg aagccggcgt
     9481 cgaagagcaa ggtccgtccg ttggcccgga ggaggaacgc gtggacgtgc agctgccatc
     9541 gcccggtggg ggagatcgtc tccggattgc tgcggcgtac cagctcccag acgtgtccgg
     9601 ggacggacgg gaaggagcgt tcgat
//
LOCUS       DASQQQ010000247         3495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_76078, whole genome shotgun sequence.
ACCESSION   DASQQQ010000247 DASQQQ010000000
VERSION     DASQQQ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76078"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1211)
                     /gene="tuf"
                     /locus_tag="VE646_09395"
     CDS             complement(27..1211)
                     /gene="tuf"
                     /locus_tag="VE646_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HZD80239.1"
                     /translation="MGKQKFERTKPHVNIGTIGHIDHGKTTLTAAITKVQAAKGLADF
                     TPFDQIDKAPEERERGITIAIAHVEYQTDARHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHVLLARQVNVPYIVVALNKVDMVDDPELLDLVELEVRELLTS
                     YEFPGDEVPVIRISALKALEGDPEAEAQVEELLQAVDTYIPEPVRDTDRPFLMSIEDV
                     FSITGRGTVVTGRVDRGVLKKMEEVEIVGIHPTRKTTATDLEMFRKLLDEVRAGDNVG
                     VLLRGIDKDEVERGQVLSRPGSITPHTDFSAQVYVLSKDEGGRHTPFFNGYRPQFYFR
                     TTDVTGTAHLPEGVEMVMPGDNVEMTVELIAPIAMEEGLRFAIREGGRTVGAGRVTKI
                     AK"
     gene            complement(1233..3314)
                     /gene="fusA"
                     /locus_tag="VE646_09400"
     CDS             complement(1233..3314)
                     /gene="fusA"
                     /locus_tag="VE646_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZD80240.1"
                     /translation="MTRRTPLERLRNIGIMAHIDAGKTTTTERILYYTGKAYKIGEVH
                     EGTAQMDWMVQERERGITITSAATTCHWKDHWINIIDTPGHVDFTVEVERSLRVLDGA
                     IAVFDAVAGVEPQTETVWRQADRYRVPRICFVNKMDRTGADFGRTVQMIVDRLHAEPL
                     VVQLPWGAEAGFQGVIDLVDMKALHWVNEMGEEWETTEIPAELQEAAAEAHHELFEKL
                     ADHDESLMDRFVHESEPTPEELRRAIRRATLAGEGVPVLCGSAFKNKAIQPLLDAIVA
                     YLPAPTDVPPVEGHTPRGEHAERRPDDGEPFSGLVFKIMSDPYVGRLTYVRVYSGLLR
                     AGSHIENSTKDRKERVGRILQMHANHREDKEAAFTGDIVAVVGLKHSTTGDTLTDPAN
                     PIVLESISFPTPVISVAVEPKTKADQDKLGTGLGKLADEDPTFVVKYDDETGQTVISG
                     MGELHLEIIVDRLRREFSVDANVGKPQVAYRETIRHPVTKVEGRFVRQTGGRGQFGHV
                     YLDLEPTGQGGGYEFVNKIVGGSIPREYIPAVDHGIQEAMESGVLAGYPLVDVRATLV
                     DGSYHEVDSSEMAFKIAGSMAVKEAARRAGPVLLEPVMEFEIVTPEDFLGDVMGDVTS
                     RRGRIERIDERSGQKAIRVLVPLAELFGYATDLRSRTQGRASHTPMQLEGYHEVPASI
                     SKEIVARVRGE"
BASE COUNT          467 a         1234 c         1167 g          627 t
ORIGIN      
        1 gtccctctcc ctctccctcg gtccggctac tttgcgatct tcgtcacgcg cccggcgccc
       61 acggtgcggc cgccctcgcg gatcgcgaag cgcaggccct cctccatggc gatgggggcg
      121 atcagctcga cggtcatctc cacgttgtcc ccgggcatca ccatctccac cccctcgggc
      181 aggtgggcgg tgccggtgac gtcggtggtg cggaagtaga actgggggcg gtagccgttg
      241 aagaacgggg tgtggcgccc gccctcgtcc ttggacagca cgtagacctg ggcggagaag
      301 tcggtgtggg gggtgatcga gccggggcgg gacagcacct ggccccgctc cacctcgtcc
      361 ttgtcgatcc cccgcagcag gacccccacg ttgtccccgg cccgcacctc gtccaggagc
      421 ttgcggaaca tctccaggtc ggtggcggtg gtcttgcggg tggggtggat cccgacgatc
      481 tccacctcct ccatcttctt gagcacgccg cggtccaccc ggccggtgac cacggtgccc
      541 cgcccggtga tcgagaacac gtcctcgatc gacatcagga aggggcggtc ggtgtcgcgg
      601 accggctcgg ggatgtaggt gtcaaccgcc tggagcagct cctccacctg ggcctcggcc
      661 tcggggtccc cctccagggc cttcagcgcg ctgatccgga tcaccgggac ctcgtcgccc
      721 gggaactcgt aggaggtcag cagctcccgg acctccagct ccaccaggtc cagcagctcg
      781 gggtcatcga ccatgtccac cttgttcagg gccaccacga tgtaggggac gttgacctgg
      841 cgggccagca gcacgtgctc ccgggtctgg ggcatcgggc cgtcggcggc cgagaccacc
      901 aggatcgccc cgtccatctg ggccgccccg gtgatcatgt tcttgatgta gtcggcgtgg
      961 ccggggcagt ccacgtgggc gtagtggcgg gcgtcggtct ggtactcgac gtgggcgatc
     1021 gcgatcgtga tcccccgctc ccgctcctcc ggagccttgt cgatctggtc gaagggggtg
     1081 aagtcggcca gccccttggc cgcctggacc ttggtgatgg ccgcggtgag cgtcgtcttg
     1141 ccgtggtcga tgtgcccgat cgtgccgatg ttcacgtggg gcttggtccg ctcgaacttc
     1201 tgcttgccca tgtcgtgctc cctctcctcc cgctactccc cgcgcacgcg ggccacgatc
     1261 tccttggaga tgctggccgg cacctcgtgg tacccctcga gctgcatggg cgtgtgcgac
     1321 gcccggccct gggtccgcga acgcaggtcg gtcgcgtacc cgaagagctc cgccagcggg
     1381 accagcaccc ggatggcctt ctgtccgctc cgctcgtcga tccgttcgat ccgcccccgg
     1441 cgtgacgtga cgtcacccat cacgtcgccc aggaagtcct ccggcgtgac gatctcgaac
     1501 tccatcacgg gttcgagcag gaccggtccc gcccggcgag cggcctcctt cacggccatc
     1561 gagccggcga tcttgaacgc catctcggac gagtccacct cgtggtagga cccgtccacc
     1621 agcgtggccc gcacgtcgac cagcgggtag ccggccagga cgccggactc catcgcctcc
     1681 tggatgccgt ggtccacggc cgggatgtac tcgcgcggga tcgacccgcc gacgatcttg
     1741 ttcacgaact cgtagccgcc cccctggccg gtgggctcca ggtcgagata gacgtggccg
     1801 aactgcccac ggcccccggt ttggcggacg aaccgcccct ccaccttcgt caccgggtgg
     1861 cggatggtct cgcggtaggc gacctgcggc ttgccgacgt tggcgtccac gctgaactcg
     1921 cggcggaggc ggtccacgat gatctccagg tggagctcgc ccatccccga gatcaccgtc
     1981 tggccggtct cgtcgtcgta cttgacgacg aacgtggggt cctcgtcggc gagcttgccg
     2041 aggccggtcc ccagcttgtc ctggtccgcc ttggtcttgg gctcgacggc caccgagatc
     2101 accggcgtcg ggaagctgat ggactccagg acgatcgggt tggccggatc ggtcagcgtg
     2161 tcgccggtgg tcgagtgctt cagacccacc accgccacga tgtccccggt gaaggcggcc
     2221 tccttgtcct cgcggtggtt cgcgtgcatc tgaaggatgc ggccgacccg ctccttccgg
     2281 tccttcgtgg agttctcgat gtgcgagccg gcgcggagga ggcccgagta cacgcgcacg
     2341 tacgtcagcc gccccacgta cgggtccgac atgatcttga agaccaggcc gctgaacggc
     2401 tccccgtcgt cgggcctgcg ttcggcgtgc tcgccccggg gcgtgtgccc ctcgacgggc
     2461 gggacgtcgg tgggggccgg caggtacgcg acgatcgcgt ccagcagcgg ctggatcgcc
     2521 ttgttcttga aggcgctgcc gcacagcacg gggacaccct cgccggccag cgtggcccgc
     2581 cggatcgccc ggcggagctc ctcgggcgtc ggctcggact cgtgcacgaa cctgtccatc
     2641 agggactcgt cgtggtcggc gagcttctcg aacagctcgt ggtgcgcctc ggccgcggcc
     2701 tcctgcagct cggcggggat ctcggtcgtc tcccactcct cgcccatctc gttgacccag
     2761 tggagggcct tcatgtccac caggtcgatc acgccctgga agccggcctc cgcgccccac
     2821 gggagctgca ccaccagtgg ctcggcatgc agccggtcga cgatcatctg gaccgtgcgg
     2881 ccgaagtccg cgcccgtccg gtccatcttg ttcacgaagc agatccgcgg cacgcgatag
     2941 cggtcggcct ggcgccacac ggtctcggtc tgcggctcca cgccggcgac ggcgtcgaag
     3001 accgcgatgg cgccgtccag gacccgcagc gagcgctcga cctcgaccgt gaagtcaacg
     3061 tggccggggg tgtcgatgat gttgatccag tggtccttcc agtgacaggt ggtcgcggcc
     3121 gaggtgatgg tgatcccccg ctcgcgctcc tggaccatcc agtccatctg ggccgtgccc
     3181 tcgtggacct cgccgatctt gtacgccttg cccgtgtaat agagaatgcg ctcggtcgtg
     3241 gtcgttttcc cggcatcgat gtgggccatg atgccgatat tgcgcagtct ctcgagtggt
     3301 gttcgtctgg tcatgcgttc cttgaatcct atccgttcct catcgattcc aaagcgcgcg
     3361 cagagcgcgc cggacacagc cggtcgtgag ccggggatcg gccggcgacc gaacgatctc
     3421 ttatcctgga gacccgccgc cggcgccgcc atcgaggggc gcgccccgac gcggggctga
     3481 agggacaaat catgc
//
LOCUS       DASQQQ010000248         6598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_158151, whole genome shotgun sequence.
ACCESSION   DASQQQ010000248 DASQQQ010000000
VERSION     DASQQQ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158151"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VE646_09405"
     CDS             <1..557
                     /locus_tag="VE646_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80241.1"
                     /translation="VLAGATMIVGYLGFAPTKAPFLDWVFFERPEAASLNGLVAGLSI
                     LAAGTGILVAYGMYREYRERDPLLAMGPVYTLLERKYYLDDIYWKGVIQPIRDQASAF
                     ANWSNQTILDGAVNGAARLARGLSGLVNGFDRNVVDGAVNGVAETAGFFGGLLRLLQS
                     GNVQRYAAFLFTGVVILAVIFTRI"
     gene            580..2115
                     /locus_tag="VE646_09410"
     CDS             580..2115
                     /locus_tag="VE646_09410"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HZD80242.1"
                     /translation="MSGWDSSAISIATFLPLAGAVVIAFMPRERERMVRGLGIVFTGA
                     ALAVAVAIAIGFHYGGSGLQFEVNTQWIPVIGARYHVGIDGISLPLYLLTFVLSFLCA
                     IYTWRYVPSPGRAKAFLALQLLLETGMAGTFIAFDLVLFFIFWELVLVPMYFLIAIWG
                     SANREYAAIKFFLYTLFGSVFMLLGFLAMYFRSDVAGAHTFDMIQLQRFAASGGFTQG
                     FQLVVFAGIFLGFAIKVPMWPFHTWLPDAHTEAPTVGSVLLAGVLLKMGTYGFIRIAL
                     PMLPYAARKYAPWIGLLAAIAIVYAALACLAQKDLKRLIAFSSVGHMGFVMLGIATLT
                     QIGINAAIFGMVAHGVITGMLFFLVGSIYDRYHTRQIADLGGGLMQKLPRLGGVLAFV
                     AIASLGLPGLAGFWGEVMALLSAFNPAASLNTGVFRGYMVAGGIGTILTAGYFLWMLQ
                     RVNMGGLPERWKEAVLPDILAVEWVSWLPLLVATVALGVFPRLLFGVTNEAVAGLSHL
                     FGI"
     gene            2141..3601
                     /locus_tag="VE646_09415"
     CDS             2141..3601
                     /locus_tag="VE646_09415"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HZD80243.1"
                     /translation="MTIDYHAISPELFLSGTILLVLVADAFIRPRRKWLAMPLSFLGV
                     LGALIATLTLIGSDRTTFGGMYRVDNFALLFKVFFLSVALVVLAVSLRYFREGRWYQG
                     EYYYLLLTSFLGATMMPSSRDLLMLFISLELVSAPGFLIAAFRKSDPRSNEAGLKFFL
                     IGVLSTAVMLYGMSLIYGVTGSTNLAQIGQVLGGTLAAGRSTLADAAILFVVAGFAFK
                     VSAVPFQFWAPDTYEGAPVPVAAYLATASKAAGFAGLLQLMFVAFPAQWAFWTPVFAA
                     LSIATMTIGNLVALQQRQVVRLLAYSSIAQAGYMLLPFALVSANQAVNQDAFSAATTY
                     ILIYAVMTLGAFAVVIALSRESPGLQIADFRGLMRRAPVLAVAMITFMVSLAGIPPTG
                     GFWAKFLIFKVGIDRGGLGVALAIVMIVNSVASLYYYLLIPRETVFTGPEIARPFRSP
                     ALVTGVAVLATLTVLAIGVYPEIFYHYPPLSTLVGH"
     gene            3830..4711
                     /locus_tag="VE646_09420"
     CDS             3830..4711
                     /locus_tag="VE646_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HZD80244.1"
                     /translation="MIGINRAKTWILIAALGGLFVLIGDLFFGTQGAWTFLILGLAVN
                     FGLYWFSDKIAIATTRSKPVSEQEYPRLYAIVRELAGLHHMPMPKIYVSEMLQPNAFA
                     TGRSPNHASVAVTKGILQILDERELRGVLAHELSHVANRDILINSIAAGIGMSITMIA
                     RFAFFFGGSDSRDNPLGPFAFLIAWILAPIAAAIIQLAVSRSREYQADESGAILSHDP
                     QALAGALRKLETASKRVPAPQSVSPAQAHMFIVNPFSGGSRGPGRSMGRSLAGLFSTH
                     PPTDARIARLEDIARRL"
     gene            complement(4767..5441)
                     /locus_tag="VE646_09425"
     CDS             complement(4767..5441)
                     /locus_tag="VE646_09425"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZD80245.1"
                     /translation="MPEPTVRAVLLDLWDTLAWNEWPPFRDRVAERSGLSVERLRRAL
                     MQTLRLRNRGTYPDGAADWAAVFAAAGIDPVDQLGAEVAAMEADQLSRDAHLFDDSVP
                     TLRRLRAAGLRTAVVSNCSHSTRPVVDRLLLAGEADAIVLSVEVGSVKPEPDIYRAAL
                     AELGAEAQQAVFVDDQVDYCRGAEEVGIRPYLIRRGRPTWDDQDPPDPAGFAVIASLQ
                     ELPVGT"
     gene            complement(5487..>6598)
                     /locus_tag="VE646_09430"
     CDS             complement(5487..>6598)
                     /locus_tag="VE646_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZD80246.1"
                     /translation="AIRAVADQGIHFAVTTETTVALAEAICERFQLEQVRFVNSGTEA
                     TMDALRVARAATGRDRVVKMEGSYHGHHDALLFSVVPEADTLGMRDNVSGESVAKGAA
                     YTTQPTSKGIPRSMWEDTIIVPFNDAQAVEDVFAERGDQIAAVILEPVMMNIGIVVPQ
                     PGYLQALRDACDRNGSVLIFDEVKCGGTIAYGGATERFGVRPHLAAFAKAVGGGATTG
                     AFGGEASIMEVVTKGAAQQGTFNGNPLSAAAGLAALTKVLTRDAYEGLGKMGTLLAEG
                     CQRAIGETGIPAHTVDLGAKGCVSYRSEPLKDYRDFLDTLPDLYYASYPWMVNRGLFM
                     TPGDEEQWTISVQHTEEDLQRYIDAFGEFCQELVK"
BASE COUNT          845 a         2275 c         2319 g         1159 t
ORIGIN      
        1 tggtcctggc cggcgccacc atgatcgtcg gctacctggg gttcgcgccc accaaggcac
       61 ccttcctgga ctgggtgttc ttcgagcgtc cggaggcggc ctcgctgaac gggctggtgg
      121 ccggcctgtc gatcctggcc gccggcaccg gcatcctggt cgcctacggc atgtatcgcg
      181 agtaccggga gcgcgacccg ctgctggcga tgggccccgt ctacaccctc ctcgagcgca
      241 agtactacct ggacgacatc tactggaagg gcgtcatcca gcccatccgc gaccaggcct
      301 cggccttcgc caactggagc aaccagacca tcctggacgg tgcggtgaac ggcgccgctc
      361 ggctggcgcg ggggttgtcg ggcctggtga acggcttcga ccgcaacgtg gtggacggtg
      421 ccgtgaacgg cgtggccgag acggccgggt tcttcggtgg cctgctccgc ctgctgcagt
      481 ccggcaacgt gcagcggtac gcggccttcc tgttcacggg cgtggtgatc ctggccgtga
      541 tcttcacgag gatctgatga ggcgagagag ggagcgtcga tgagcgggtg ggacagctcg
      601 gcgatctcga tcgccacgtt cctgccgttg gcgggggcgg tggtgatcgc gttcatgccg
      661 agggagcggg agcggatggt ccggggcctg ggcatcgtgt tcacgggcgc cgcgctggcg
      721 gtggcggtgg ccatcgcgat cggcttccac tacggcggct ctggcctgca gttcgaggtc
      781 aacacgcagt ggatcccggt gatcggcgct cggtaccacg tggggatcga cgggatcagc
      841 ctgcccctgt acctgctcac gttcgtgctc tcgttcctgt gcgcgatcta cacctggcgc
      901 tacgtgccca gccccgggcg ggccaaggcc ttcctcgccc tgcagctcct gctggagacc
      961 ggcatggccg gcacgttcat cgccttcgac ctggtgctgt tcttcatctt ctgggagctc
     1021 gtgctggtcc cgatgtactt cctgatcgcc atctggggca gcgcgaaccg agagtacgcc
     1081 gccatcaagt tcttcctgta cacgctgttc gggtccgtgt tcatgctgct gggcttcctg
     1141 gcgatgtact tccgctccga cgtggccggg gcgcacacct tcgacatgat ccagctgcag
     1201 cgcttcgcgg cctcgggcgg gttcacccag ggcttccagc tggtggtgtt cgcggggatc
     1261 ttcctgggct tcgccatcaa ggtgcccatg tggccgttcc acacctggct gcccgatgcc
     1321 cacaccgagg cccccacggt gggctcggtc ctgctggccg gggtcctgct gaagatgggc
     1381 acctacggct tcatccggat cgccctgccc atgctgccct acgcggcccg caagtacgcg
     1441 ccctggatcg gcctgctggc ggcgatcgcg atcgtctacg ccgccctggc ctgcctggcc
     1501 cagaaggacc tgaagcggct gatcgcgttc tcctcggtcg gtcacatggg gttcgtgatg
     1561 ctggggatcg ccacgctcac ccagatcggc atcaacgcgg cgatcttcgg gatggtggcc
     1621 cacggcgtga tcaccgggat gctgttcttc ctggtgggct cgatctacga ccggtaccac
     1681 acgcggcaga tcgccgacct gggcggcggg ctgatgcaga agctcccccg gctgggcggg
     1741 gtgctggcct tcgtggcgat cgcctcgctc gggctgccgg gcctggccgg cttctggggc
     1801 gaggtgatgg cgctcctgtc ggcgttcaac ccggccgcct ccctgaacac gggcgtgttc
     1861 cggggctaca tggtggcagg ggggatcggc acgatcctga ccgccggcta cttcctgtgg
     1921 atgctccagc gggtgaacat gggtggcctg cccgagcggt ggaaggaggc cgtgctgccc
     1981 gacatcctcg cggtggaatg ggtgtcctgg ctcccgctgc tggtggccac cgtggccctg
     2041 ggggtcttcc cccgcctgct gttcggggtg acgaacgagg cggtggccgg gctgtcgcac
     2101 ctgttcggga tctgagggtg aggggagcgg ggagccggcc atgaccatcg actaccacgc
     2161 gatctcgccg gagctgttcc tgtccggcac gatcctgttg gtgctggtgg ccgacgcgtt
     2221 catccggccc cgacgcaagt ggctggccat gccgctgtcg ttcctcggcg tgctgggggc
     2281 gctgatcgcc accctcacgc tgatcggctc cgatcggacc accttcggcg ggatgtaccg
     2341 ggtggacaac ttcgccctgc tgttcaaggt gttcttcctg tcggtggcgt tggtggtgct
     2401 ggccgtctcg ctccgctact tccgcgaggg ccgctggtac cagggcgagt actactacct
     2461 gttgctgacc tccttcctgg gcgccaccat gatgccctcg tcccgggacc tgctgatgct
     2521 gttcatctcg ctggagctgg tctcggcgcc ggggttcctg atcgccgcct tccgcaagtc
     2581 cgatccccgg tccaacgagg cggggctgaa gttcttcctg atcggcgtgc tgtccaccgc
     2641 ggtgatgctc tacggcatga gcctgatcta cggggtcacc ggcagcacca acctggccca
     2701 gatcggccag gtcctgggcg ggaccctcgc ggccgggcgg agcaccctgg ccgacgccgc
     2761 gatcctgttc gtcgtggccg ggttcgcctt caaggtgagc gcggtcccct tccagttctg
     2821 ggccccggac acgtacgagg gtgcgccggt cccggtcgcc gcgtacctgg ccaccgcctc
     2881 caaggcggcc gggttcgccg ggctgctcca gttgatgttc gtggccttcc ccgcccagtg
     2941 ggcgttctgg acgccggtgt tcgccgcgct gtccatcgcc accatgacca tcgggaacct
     3001 ggtggccctc cagcagcggc aggtggtgcg gctgctcgcc tactccagca tcgcgcaggc
     3061 cggctacatg ctgctgccgt tcgcgctggt ctcggcgaac caggccgtga accaggacgc
     3121 gttctccgcg gccaccacct acatcctgat ctacgcggtg atgacgctgg gcgcgttcgc
     3181 ggtggtgatc gcgctgtccc gggagtcccc ggggctgcag atcgccgact tccgagggct
     3241 gatgcgccgg gccccggtgc tggccgtggc catgatcacc ttcatggtgt ccctggctgg
     3301 gatcccgccc acgggcgggt tctgggcgaa gttcctgatc ttcaaggtgg gcatcgaccg
     3361 cggcggcctg ggtgtggccc tggccatcgt gatgatcgtg aactcggtgg ccagcctgta
     3421 ctactacctg ctgatccccc gggagacggt gttcacgggg cccgagatcg cccggccctt
     3481 ccgctccccc gccctggtga ccggcgtggc ggtgctggcc accctgaccg tgctggcgat
     3541 cggcgtgtat cccgagatct tctaccacta cccgccgctg tccaccctgg tcgggcactg
     3601 accccgaccg cgctcgggcc ttcccatgca gtggggagcg ctgaacctgg gctccggtca
     3661 tggcgaacgg ggtgtgagaa catgggagca ggtgcaaggg gagtagccca cggcggggag
     3721 ccgtcaaccc gctccggtga cggagccggc ccccgcggga gcgcggaccc ggcaagacct
     3781 tcgcctgatg ggcggaggtc ttcgtgcttg ttgaaggagg aatgacgacg tgatcgggat
     3841 caaccgggcc aagacctgga tcctcatcgc tgcgttgggc gggctgttcg tgctgatcgg
     3901 cgacctgttc ttcggcaccc agggcgcctg gacgttcctg atcctcgggc tggccgtgaa
     3961 cttcggcctg tactggttct cggacaagat cgccatcgcc accacgcggt ccaagcccgt
     4021 gtccgagcag gagtacccgc gcctgtacgc gatcgtgcgg gagctggcgg ggctgcacca
     4081 catgccgatg ccgaagatct acgtgtccga gatgctccag ccgaacgcct tcgcgaccgg
     4141 ccggagcccg aaccacgcct cggtggccgt gaccaagggc atcctgcaga tcctggacga
     4201 gcgcgagctg cgcggcgtcc tggcccacga gctgtcccac gtggccaacc gcgacatcct
     4261 gatcaactcg atcgcggccg gcatcgggat gagcatcacg atgatcgcgc gcttcgcgtt
     4321 cttctttggc ggcagcgaca gccgcgacaa cccgctgggg ccgttcgcct tcctgatcgc
     4381 ctggatcctg gcccccatcg cggcggccat catccagttg gcggtctcgc ggtcccgcga
     4441 gtaccaggcc gacgagtccg gcgcgatcct ctcccacgac ccgcaggccc tggccggcgc
     4501 gctgcgcaag ctggagaccg cgtcgaagcg ggtgccggcg ccgcagagcg tgagcccggc
     4561 gcaggcccac atgttcatcg tgaacccgtt ctcgggcggc tcccgcgggc ccgggcggag
     4621 catgggccgg tcgctggccg ggctgttctc cacgcacccg ccgaccgatg ctcggatcgc
     4681 gcggctggag gacatcgccc ggcgtctgtg agggcgccgc tggtcggatc ctggatgggg
     4741 cccggggcgt ggcgctccgg cccccgtcac gttcccacgg gcagctcctg caggctcgcg
     4801 atgaccgcga accccgcggg atcgggcggg tcctggtcgt cccaggtcgg ccggccccgg
     4861 cggatcaggt aggggcggat ccccacctcc tcggcgccgc ggcagtagtc cacctgatcg
     4921 tccacgaaga cggcctgctg cgcctcggct cccagctcgg cgagcgccgc ccggtagatg
     4981 tcgggctcgg gtttcacgct gccgacctcg accgagagga cgatcgcgtc ggcctcgccg
     5041 gccagcagca accggtccac caccggccgg gtggagtggg agcagttgct gaccacggcg
     5101 gtgcgcaggc ccgcggcgcg gagccgccgc agcgtgggga ccgagtcgtc gaacaggtgg
     5161 gcgtcgcggc tcagctggtc ggcctccatg gcggcgacct cggccccgag ctggtccacc
     5221 gggtcgatcc cggcggcggc gaacacggcc gcccagtcgg ccgccccgtc cgggtaggtc
     5281 cctcggttgc gcaggcgcag ggtctgcatc agggcccgac ggagccgctc caccgagagc
     5341 cccgagcgct cggccacgcg gtcccggaag ggaggccact cgttccaggc cagcgtgtcc
     5401 cagaggtcga gcaggacggc acggaccgtg ggctcgggca ccggttcagc ggcgcggctc
     5461 ggcgatcccg atcggcgtcg ccccggctac ttgaccagct cctggcagaa ctcgccgaag
     5521 gcgtcgatgt agcgctgcag gtcttcctcg gtgtgctgga ccgagatggt ccactgctcc
     5581 tcgtcgccgg gggtcatgaa caggccgcgg ttcaccatcc acgggtagga cgcgtagtac
     5641 aggtcgggca gcgtgtccag gaagtcccgg tagtccttca gcggctccga ccggtaggac
     5701 acgcagccct tggcccccag gtccacggtg tgggcgggga tcccggtctc gccgatggcc
     5761 cgctggcagc cctcggccag cagggtgccc atcttgccca ggccctcgta cgcgtcacgg
     5821 gtcaggacct tggtcagggc ggccagcccg gccgccgccg agagtgggtt gccgttgaag
     5881 gtgccctgct gggcggcgcc cttggtcacg acctccatga tcgaggcctc gccgccgaag
     5941 gcgccggtgg tggcgccgcc gcccacggcc ttggcgaagg cggccaggtg gggccggacc
     6001 ccgaaccgtt cggtggcccc gccgtaggcg atggtgccgc cgcacttgac ctcgtcgaag
     6061 atcaggacgg agccgttgcg gtcgcacgcg tcccgcagcg cctgcaggta gccgggctgg
     6121 gggaccacga tcccgatgtt catcatcacc ggctcgagga tcaccgcggc gatctggtcg
     6181 ccccgctcgg cgaacacgtc ctccacggcc tgggcgtcgt tgaaggggac gatgatggtg
     6241 tcctcccaca tcgagcgcgg gatgcccttg gaggtgggct gtgtggtgta ggcggccccc
     6301 ttggccacgg actcgccgct cacgttgtcc cgcatgccca gcgtgtcggc ctcggggacc
     6361 acggagaaca gcagggcgtc gtggtggccg tggtaggacc cctccatctt gaccacccgg
     6421 tcccgaccgg tggcggcccg ggccacgcgc agggcgtcca tggtggcctc ggtgccggag
     6481 ttcacgaacc gtacctgctc cagctggaag cgctcgcaga tggcctcggc cagcgcgacc
     6541 gtggtctcgg tggtgacggc gaagtggatg ccctggtccg ccacggcccg gatcgcct
//
LOCUS       DASQQQ010000249         9218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_44689, whole genome shotgun sequence.
ACCESSION   DASQQQ010000249 DASQQQ010000000
VERSION     DASQQQ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9218
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44689"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VE646_09435"
     CDS             <1..237
                     /locus_tag="VE646_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80247.1"
                     /translation="PVALVMDRPSWWRARSMVGAMVRGRGEISIPGRLRTGSGSARQR
                     VRLTGAEPTGAALVRLRPERLVWWSGWESGTVPR"
     gene            234..701
                     /locus_tag="VE646_09440"
     CDS             234..701
                     /locus_tag="VE646_09440"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZD80248.1"
                     /translation="MTVVEATVEVMAPPDRVWAVVSDPRNLPRWDRRVASVEDVPSQG
                     LSRGATYTTVMRFMAVRSRVHAEVLEWDPPERATVRLMGLLEATVSTTVTALPGGRSR
                     LRHIVDYHFPGGALGDLAARSLRVLGGAGFALRHGTLAQVRQIERGASEAEPG"
     gene            736..1347
                     /locus_tag="VE646_09445"
     CDS             736..1347
                     /locus_tag="VE646_09445"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZD80249.1"
                     /translation="MLKRAARVAGWAGLCLAAGVAVTRGHARRPDERAFRLVNRGAGD
                     AVDRLFGWGTELGSIWASAAAAAVLAATGHRRAAARGLGAASVAWVAGQGLKELFPRL
                     RPYQAEGAAVRLLVAPPRGRSWPSSHPAVLSAFLTVAGRELGLPVRARAALGALGAAV
                     GFSRTYVGVHYPGDVLGGLSMGRLVADALSEAPGSSWRLRPPQ"
     gene            complement(1341..2471)
                     /locus_tag="VE646_09450"
     CDS             complement(1341..2471)
                     /locus_tag="VE646_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HZD80250.1"
                     /translation="MVTQEQVRQALRGVLDPEIGRPIEDLGMLQAIDIENGLVRVHVL
                     LTIAGCPLQERITADVTNALRPIGVERVDVRMTPMSEEQRAELVGKLRGAAPAQQRTF
                     FTGEQTSVIAVASGKGGVGKSSVTVNVAAALAAEGHRVGILDADVWGFSVPRMLGVTG
                     KPVGFNDMILPLESHGVKVISMGFFVPEETPVIWRGPMLHKAIEQFLGDVFWGDLDFL
                     LCDLPPGTGDVSISLASFLPGASMLVVTTPQEAARKVAERAGKMAERTNLRPQGVIEN
                     MSWFVCPHCGERTEIFGQGGGLEAAETLGVPLLGQVPLMPSLREGGDAGTPIVLTDPD
                     SPAGAALRDAARRLAQITRTKVGKPLPIMAQPGAAAGGVHHH"
     gene            2554..3240
                     /locus_tag="VE646_09455"
     CDS             2554..3240
                     /locus_tag="VE646_09455"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZD80251.1"
                     /translation="MATADQNTIEVHKALADDTRFRLYRYLGLSGRPVSVRELATRLS
                     LHPNTLRPHLRRLERAGLVVREARRGSSVGRPQTLYAAVDREMPEGRDYRLLAEILAG
                     LVRGQRSLDRAQELAREWGQYLVAQGGPKPGTRLPAGRNLALLQEAMARAGFDPRFHR
                     RGRQGVEISLRGCPARDLLDDHRDAVCAVHRGLLEGMLGGLKPPLRLGEFAPLVERST
                     CRLVARDVGA"
     gene            3342..3562
                     /locus_tag="VE646_09460"
                     /pseudo
     CDS             3342..3562
                     /locus_tag="VE646_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
     gene            complement(3616..4074)
                     /locus_tag="VE646_09465"
     CDS             complement(3616..4074)
                     /locus_tag="VE646_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80252.1"
                     /translation="MHRLTVRGARAGSARGLLSHALRLVLLAASLALLWRAEGALEEW
                     QRRAGIELEFKTGLWFSWVVPAVLAGILFGLALWLPRGRSAYRWTRTIVLVLPPLLLL
                     LHYWVVFGLGLDLPGALGQETPFWRSGPQFALAVFVGIGVTSGFAEPDEP"
     gene            complement(4078..5013)
                     /locus_tag="VE646_09470"
     CDS             complement(4078..5013)
                     /locus_tag="VE646_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZD80253.1"
                     /translation="MTGALIGLGIFLVLLILIVPNAIRIVREYQRLVVFRLGRCIGMK
                     GPGFVLLIPIIDRAVWVDLRELYLEIPHQTAITEDNAPISIDFIVFYKVVEAEQSVLA
                     VGNFAGAAQNIAATTLRSVVGDMALDDVLAKREQMNEILRVKLDEVTERWGVKVTNVE
                     IREIIPPPAVQEAMTRQMSAERTRRAVVTEAEGQKQAAITVAEGNKQSAILNAEGDRQ
                     AAILRAEGFSLALSKIFEVARDLDPNTMSLQYLDALKQIGASASTKFVIPLELASLLS
                     KMAGQAGRAFGDGHGGAASPGSEGGGTQQRPAERS"
     gene            complement(5010..6353)
                     /locus_tag="VE646_09475"
     CDS             complement(5010..6353)
                     /locus_tag="VE646_09475"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HZD80254.1"
                     /translation="MQRGLRAAVLGAAALALAASPAAGAWAQETTAPRVVELRLDGVV
                     DPFMADYIAGGVRAAESSSAQAVLLTIDTPGGLDSSMRQIIQAISNARVPVVCYVSPS
                     GARAASAGTFIMMGCPVAAMAPGTNIGAAHPVGVAGAIEQKKVTNDAAAYIKSLAEEH
                     HRDAAWAQKAVTQAVSISATDALQMNVIDLMAPNITQLLKDVDGRTVALGGGRTVTLR
                     TAGATLEHRGLGIGASILHTLLSPDFSFLFFYLGLGLIVIEFLHPGLSIPGILGALCL
                     VAAFVTFGVLPVQLVGIALLLASALFFLLELKHPGIGLPMVAGVVSLVFGGLTLFNPT
                     VPNAQVSLWVIVPVAAFLALFFATIVPAALRARHLPPSGGSEGMVGREGLVERELDPE
                     GVALVASEEWTARSPSGPLPRGSRIRVVGVDRLTLIVEPIVEQAPAAPGPEGGNR"
     gene            6547..7068
                     /locus_tag="VE646_09480"
     CDS             6547..7068
                     /locus_tag="VE646_09480"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZD80255.1"
                     /translation="MEGLLAGNEDAVRALYARFSRPVFTLGLRLLGSEESAEELTQDV
                     FLTAWRKAARFDATRGRLSTWLMTIAHNLAVDRLRRETGVTRPTLVLVEKIPEVPSAD
                     EEEPLMERDVALRALEGLSDAERRLLGRAYFAGLTAREIAEADGIPLGTVKTRLRTAL
                     IKVRRANQDKERP"
     gene            7065..7844
                     /locus_tag="VE646_09485"
     CDS             7065..7844
                     /locus_tag="VE646_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZD80256.1"
                     /translation="MNCLSIREQLPEFAVGVLPPEERIDVERHLQWCAGCRKEAGDLG
                     EAAATFGLALRPVDPPGELADRVVSSVARASAKPGSKRRLRAVAASTVAAAVAVAGLG
                     WGAVMAGRAERFGDRAVRAERQKAAALARFKDVLGPHGLAQLPQDHTVLGQLAPVSPA
                     RVGSGWVLQLLSPAKLDFVMVILNGIDAPPSDLPFQVQLRNAAGQTLNAGKITSLDAD
                     GGAEAYRRFANKDLTGFTNVIVTDAHGDVVLRGTVDQSAGG"
     gene            7929..>9218
                     /locus_tag="VE646_09490"
     CDS             7929..>9218
                     /locus_tag="VE646_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HZD80257.1"
                     /translation="MPEMTDVVGIGLAGGQGIRARPLTLKAPGYLRSKAAMSFLGRRL
                     IRWVIQILSSEGIKDYYIVAHGKENRYQIKVMIGYGEAFGVDVKYSPVKYDALTRGSA
                     DSTLRMLEHWDVSRTALIFPTDSIIDFDLGPMFQSHVSTGAVATIAAMTREPDEVAEK
                     YGLMLTEPDGRITDFVEKPTLAELREHFHAETDEAFVQLPLMTNAGFYLVDASVLRSL
                     ADDPAVRAMRERRLDFGKDLLPWLVESGHPVYAFPVRRIGDLGNVQDYIETMTDALRG
                     NFESVDRLLGPPFDPERHVWIAPESLAMRDSTSGATLAEKIADGSVRIGPSVRIGRFC
                     EIHPGVRIVESNLDDDIEVHRDAAIERTQIRDGAIVGAAARISEAVVGSMSEVRSEPY
                     NQTMIEDGVALGDEVIVYPGVHLSGDVSVYPRLKLPSG"
BASE COUNT         1353 a         3223 c         3326 g         1316 t
ORIGIN      
        1 ccggtggcct tggtgatgga ccgtccctcc tggtggcgag cgcgatcgat ggtgggcgcc
       61 atggttcgcg gtcggggaga gatctcgatc cccgggcgtc tgcgcaccgg ctcgggctcg
      121 gcccgacagc gggttcgcct gacgggcgcc gagccgaccg gggcggcgct ggtccggctg
      181 cggcccgaac ggctggtgtg gtggtcgggg tgggagagcg ggacggtgcc tcggtgacgg
      241 tcgtcgaggc caccgtcgag gtgatggccc ccccggatcg ggtctgggcc gtggtgtccg
      301 atccgcggaa cctgccccga tgggatcggc gtgtggcatc ggtggaggac gtcccgtccc
      361 agggcctgtc ccggggcgcc acgtacacga ccgtgatgcg gttcatggcg gtgcggtccc
      421 gggtccatgc agaggtcctg gagtgggacc cgcccgagcg ggccacggtc cgcctgatgg
      481 gtctgctgga ggcgaccgtc tccaccacgg tcaccgcgct gcccggaggg cgcagccgcc
      541 tcaggcacat cgtggattac cacttcccgg gtggggcgct cggggacctg gccgcgcgga
      601 gcctccgggt gctcggcggg gcggggttcg cgctccgtca cgggacgctg gcgcaggtcc
      661 gtcagatcga acggggagcc tcggaggccg agccgggatg acagccgacc gcctcgtgcc
      721 gcatcatccc ggccgatgct gaagcgagca gcccgggtgg ccggctgggc cggcctgtgt
      781 ctggccgccg gcgtagccgt gacccggggg catgcacggc gacccgatga gcgggcgttc
      841 cggctcgtga accgtggtgc gggcgacgcg gtcgatcggc tgttcggatg ggggaccgag
      901 ctgggatcga tctgggcctc ggccgccgcc gccgccgtcc tggctgcgac gggccatcgg
      961 cgggctgccg ctcggggctt gggggccgcc tcggtggcct gggtcgccgg gcaggggctg
     1021 aaggagctgt tcccacgcct gcgcccgtac caggcggagg gcgccgcggt gcggctgctg
     1081 gtggctccac ccaggggccg gtcctggccg agcagccatc ccgcggtgct gtcggccttc
     1141 ctcaccgtgg ctgggcgcga gctcggcctg ccggtgcgcg cccgggccgc gctcggcgcg
     1201 ctcggagcgg ccgtgggctt ctcgcgcacc tacgtggggg tccattaccc cggggacgtc
     1261 ctgggggggc tgtcgatggg acggctggtg gccgacgcgc tcagcgaggc cccggggagc
     1321 tcgtggaggc tccggccccc tcagtgatga tggacgccgc cggccgcggc gcccggttgt
     1381 gccatgatcg gcaggggctt gcccaccttg gtccgggtga tctgggccaa ccgccgggcc
     1441 gcgtctcgga gcgccgctcc ggccggtgaa tccggatcgg tcagcacgat gggggtcccg
     1501 gcgtcccctc cctcacgcag ggacggcatg agcggaacct gaccgagcag gggaacgccc
     1561 aaggtctccg cggcctccag gccgccgccc tgcccgaaga tctccgtccg ctcgccgcag
     1621 tgggggcaga cgaaccacga catgttctcg atgaccccct gagggcgcag gttcgtgcgc
     1681 tcggccatct ttccggcgcg ctccgccacc ttcctggcgg cctcctgcgg cgtggtgacc
     1741 acgagcatcg acgcgcccgg caggaaggac gccagcgaga tcgacacgtc tccggtcccc
     1801 ggcggcaggt cgcacaggag gaagtcgagg tccccccaga acacgtcgcc caggaactgc
     1861 tcgatggcct tgtgcagcat ggggccgcgc cagatcacgg gggtctcctc ggggacgaag
     1921 aagcccatcg agatgacctt cacgccgtgg gactcgaggg gcaggatcat gtcgttgaac
     1981 cccaccggct tgccggtcac ccccagcatg cgaggaaccg agaaccccca gacgtcggca
     2041 tcgaggatgc ccacgcggtg gccctcggcg gcgagggcgg ccgccacgtt gaccgtcaca
     2101 ctggacttgc cgaccccgcc cttgcccgag gccaccgcga tcaccgaggt ctgctcgccg
     2161 gtgaagaagg tgcgctgctg ggcgggcgcg gcaccgcgga gcttgcccac cagctcggcc
     2221 cgttgctcct cgctcatcgg cgtcatccga acatccaccc gctccacgcc gatcgggcgc
     2281 agggcgttcg tgacatcggc ggtgatccgc tcctggagcg ggcatcccgc gatcgtgagc
     2341 agcacgtgga cccggaccag gccattttcg atgtcgatcg cctgcagcat gccgagatcc
     2401 tcgatgggcc gcccgatctc gggatcgagc acgccccgga gcgcttggcg gacctgttct
     2461 tgcgtgacca cggggaactc cttcggatta tgctgatttg gaccgtgcga accgtccgaa
     2521 ctcctatggt acggcgctcg gcgaggtgcg tcgatggcga ccgctgacca gaacaccatc
     2581 gaggtccaca aggcgctcgc cgacgacacg cggttccgac tctaccgata cctcggcctg
     2641 tccgggcgac ccgtgtcggt gcgcgagctg gccacgcggc tgtccctgca tccgaacacc
     2701 ctccgccccc acctgcgccg gctcgagagg gccggcctgg tggtccgcga ggcccgtcgg
     2761 ggctcatccg tcggccgccc gcagacgctg tacgcggccg tggatcggga gatgccggag
     2821 ggtcgcgact accggctgct ggccgagatc ctggccggcc tggtgcgcgg gcagcggtcg
     2881 ctggaccggg cccaggagct ggcccgcgag tgggggcagt acctggtggc tcagggcggg
     2941 cccaagcccg gcaccaggct cccggcgggc cggaacctgg ccctgctgca ggaggcgatg
     3001 gccagagcgg ggttcgatcc ccggttccac cgccgggggc gccagggcgt ggagatctcg
     3061 ttgcgcggat gcccggcccg tgacctgctc gacgaccatc gcgatgccgt gtgcgcggtg
     3121 caccggggcc tgctggaggg catgttgggc ggcctgaagc cgccgttgcg cctgggagag
     3181 ttcgctcccc tggtggagcg gtccacctgc cggctggtcg ctcgggacgt cggggcctga
     3241 gcggactcag gctcgggtcc tcccctccga tacccggcct ccaggaactg aggttgcccg
     3301 ccgtggtgcg ttccctggcc gacgagccgc gggggctggt cctgttccga gacggtcagg
     3361 cctcgatcga ccaacgggag ctgggcaacg acagcgagag cttcgtctcc gttgcgggcg
     3421 gggctccgcc aggaccccga cgtgatcctg atcggcgaga tgcgggacac ggaaacgatg
     3481 cgagcgcagg cgggcggccg gggccggtca tctggtcctc tccaccctcc acacggtgga
     3541 cgccacggag agcgtgaacc gaacccggcc tgagggtcgt ggaaccgccg ccgctcgggc
     3601 tcggcgggag gtcgctcaag gctcatccgg ttccgcgaag cccgacgtca ccccgatgcc
     3661 cacgaacacc gccagggcga actgcgggcc cgagcgccag aacggcgtct cctgaccgag
     3721 ggcaccgggc aggtcgaggc ccaggccgaa cacgacccag tagtggagga gcagcagcag
     3781 tggaggcagc accaggacga tggtccgggt ccagcggtac gcgctgcgcc cccggggcag
     3841 ccacaacgcc aggccgaaga ggatgcccgc caggacggcc ggcaccaccc acgagaacca
     3901 caggcccgtc ttgaactcga gctcgatgcc ggcgcgtcgc tgccactcct ccagggcgcc
     3961 ctccgcccgc cacaggaggg ccagggatgc cgccagcagg acgagccgga gggcgtggga
     4021 gagcagccct cgggcggaac cagcccgggc tccgcgtacc gtcaggcggt gcacctctta
     4081 agatcgctcc gcgggccgct gctgggtgcc gccgccttcg gagcccgggc tcgcggcccc
     4141 gccgtgcccg tcgccgaacg cccgtccggc ctgtccggcc atcttcgaca ggagcgaggc
     4201 cagctccagg gggatcacga acttcgtgga ggccgacgcc ccgatctgtt tgagggcgtc
     4261 caggtactgc aggctcatcg tgttcgggtc caggtcgcga gcgacctcga agatcttcga
     4321 cagggccagc gagaagccct cggcgcgcag gatggccgcc tgccggtctc cctcggcgtt
     4381 caagatggcg gactgcttgt tgccctcggc cacggtgatc gcggcctgct tctggccctc
     4441 ggcctccgtc acgaccgccc ggcgcgtccg ctcggcggac atctgccggg tcatggcctc
     4501 ctgcaccgcg ggcgggggga tgatctcgcg gatctccacg ttcgtgacct tcacgcccca
     4561 gcgctcggtg acctcgtcca gcttgacgcg caggatctcg ttcatctgct cgcgcttggc
     4621 cagcacgtcg tccagggcca tgtcgccgac caccgagcgg agcgtggtgg ccgcgatgtt
     4681 ctgggccgca ccggcgaagt tgcccacggc cagcaccgac tgctcggcct ccaccacctt
     4741 gtagaagacg atgaagtcga tcgagatcgg cgcgttgtcc tcggtgatgg ccgtctggtg
     4801 cgggatctcg aggtacagct cgcgcagatc tacccacacg gcgcggtcga tgatggggat
     4861 cagcaggacg aaccccggcc ccttcatccc gatgcagcga ccgagccgga agaccaccag
     4921 gcgttggtac tcgcggacga tgcggatggc gttgggcacg atcaggatca gcaacaccag
     4981 gaagatcccc aggccgatca gtgctccggt catcggttcc ccccttccgg ccccggcgcg
     5041 gccggagcct gctccacgat cggttcgacg atcagcgtca gccgatccac gcccaccacc
     5101 cggatccggc tcccccgggg aagcgggccc gagggcgagc gggcggtcca ctcctcggat
     5161 gccaccagcg ccaccccctc cggatcgagc tcccgctcca ccaggccctc gcggcccacc
     5221 atgccctccg agccgccgga ggggggcagg tggcgggccc ggagggctgc ggggacgatc
     5281 gtggcgaaga agagcgccag gaacgcggcg accgggacga tcacccacag ggagacctga
     5341 gcgttcggga cggtggggtt gaacagcgtg agcccaccga acacgagcga cacgaccccc
     5401 gccaccatgg gcagcccgat gcccggatgc ttcagctcca gcaggaagaa cagcgccgaa
     5461 gccagcagca gggcgatgcc cacgagctgc acgggcagca cgccgaaggt cacgaaggcc
     5521 gccaccaggc acagggcccc gaggatgccg gggatcgaga gccccggatg caggaactcg
     5581 atcacgatca gcccgaggcc caggtagaag aacaggaagg agaagtcggg agagagcagc
     5641 gtgtgcagga tgctcgctcc gatccccagg cctcggtgct cgagcgtcgc cccggccgta
     5701 cgcagcgtga cggtccggcc gcctcccagg gcaaccgtcc gtccgtcgac gtccttcaac
     5761 agctgggtga tgttcggtgc catcaggtcg atcacgttca tctgcagggc atcggtggcc
     5821 gagatcgaca cggcctgcgt gacggccttc tgggcccagg cggcgtcccg gtgatgctct
     5881 tcagccaggc tcttgatgta cgccgcggcg tcattggtca ccttcttctg ctcgatcgcg
     5941 cccgccacgc ccaccggatg ggcggccccg atgttcgtac cgggcgccat ggccgccacg
     6001 gggcagccca tcatgatgaa cgtgccggcg gaggccgctc tggccccgga cggcgagacg
     6061 tagcagacca ccgggacgcg ggcgttcgag atcgcctgga tgatctggcg catcgaggag
     6121 tccagccctc cgggggtatc gatggtgagc aacaccgcct gggccgaaga gctctcggcg
     6181 gcccgaacac ccccggcgat gtagtcggcc atgaaggggt cgaccacgcc gtccagccgg
     6241 agctccacca cccgcggggc ggtcgtctcc tgggcccatg ccccggccgc gggcgacgcg
     6301 gccagggcca gtgccgcagc acccaacacc gcagctcgca gcccccgttg catgcatcca
     6361 tcatcccacc gagggggggc cgccggaagc cgcgaacggt cgaccgatgg gcgaaccggg
     6421 tgggatgatg gatgcggagg tcggaggcaa gctcgggcct cccggtcccg cgtatatccc
     6481 gaagggagag gccgagtgaa tgctcgggcc gaaggtccac tcctcccacg agaggaacaa
     6541 caactggtgg agggcctgct ggccggcaac gaggacgctg ttcgcgcgct gtacgcgagg
     6601 ttctcgcgcc ccgtgttcac actcggtctc cgcctgctcg gcagcgagga gtcggccgag
     6661 gagctcaccc aggacgtctt cctcaccgcc tggcggaagg cggcccggtt cgacgccacc
     6721 cgcggacgcc tgtccacgtg gctgatgacg atcgcgcaca acctggccgt ggaccgactg
     6781 cggcgggaga ccggggtgac gcggcccacg ctggtgctgg tggagaagat cccggaggtc
     6841 ccctctgccg acgaggagga gcccctgatg gagcgggacg tggccctgcg cgccctggag
     6901 ggtctctccg acgccgagcg ccgactcctc ggccgcgcgt acttcgccgg tctgaccgcg
     6961 cgggagatcg ccgaggcgga cgggatcccg ctgggtacgg tgaagacgcg gctgcggacc
     7021 gcgctgatca aggtccgccg tgccaaccaa gacaaggaac gcccgtgaac tgtctctcga
     7081 tccgtgagca actgcccgag ttcgccgtcg gcgtcctgcc tcccgaggag cgcatcgacg
     7141 tcgagcgaca cctgcagtgg tgcgccggct gcaggaagga ggccggggac ctcggcgagg
     7201 ctgcggccac cttcggcctc gcgttgagac cggtcgaccc tcccggggag ctggccgacc
     7261 gcgtggtgag ctcggtcgcg cgcgcctcgg ccaagccggg aagcaaacgc cggttgcgcg
     7321 ccgtggccgc ctcgacggtg gcggccgcgg tggccgtggc ggggctgggc tggggcgccg
     7381 tgatggccgg tcgggccgag cgcttcggag accgggcggt tcgggccgag cggcagaagg
     7441 ccgccgccct ggcccggttc aaggatgtgc tcggtcccca tgggctcgcc cagctgccgc
     7501 aggaccacac ggtcctggga cagctcgccc ccgtgtcgcc ggcccgggtg gggagcggtt
     7561 gggtgctgca gctgctctcg ccggccaagc tcgacttcgt gatggtgatc ctgaacggga
     7621 tcgatgctcc gccctccgac ctgcccttcc aggtgcagct gcgcaacgcg gccggtcaga
     7681 ccctcaacgc cggcaagatc acctccctgg atgcggatgg cggggccgag gcgtaccggc
     7741 ggttcgcgaa caaggacctc accgggttca ccaacgtcat cgtgaccgac gcccatgggg
     7801 acgtcgtgct ccgcgggacg gtggaccagt cggccggcgg ctgagcggca gctgatcccg
     7861 gggcctcggt cggacgctcg gtcccggacg ctcggtccgg ggacggcatc ccggatatca
     7921 tgcggcccat gcccgagatg accgacgtgg tgggcatcgg cctggccggg ggacagggca
     7981 tccgagcccg gccgctcacg ctgaaggcgc ccgggtacct ccggtcgaaa gcggcgatgt
     8041 ccttcctggg gcgccggttg atccggtggg tgatccagat cttgtcgagc gaggggatca
     8101 aggactacta catcgtcgcc cacggcaagg agaaccggta ccagatcaag gtcatgatcg
     8161 ggtacggaga ggccttcggc gtggacgtga agtactcgcc ggtgaagtac gacgcgctga
     8221 ccaggggcag cgccgattcc acgctccgga tgctcgagca ctgggacgtc agccggaccg
     8281 cgctgatctt ccccaccgac tcgatcatcg acttcgacct cggccccatg ttccagtccc
     8341 acgtgtccac cggcgccgtg gccacgatcg ccgcgatgac ccgggagccc gacgaggtgg
     8401 ccgagaagta cgggctgatg ctgaccgagc cggacggccg gatcaccgac ttcgtggaga
     8461 aaccgaccct ggccgagctg cgcgagcact tccacgccga gaccgatgag gcgttcgtcc
     8521 agctcccact gatgaccaac gccggcttct acctcgtgga cgcctcggtg ctccggagcc
     8581 tcgccgacga ccccgccgtg cgggccatgc gcgagcggag gttggacttc ggcaaggacc
     8641 tgctgccctg gctggtcgag agcggccacc ccgtctacgc cttccccgtc cgccggatcg
     8701 gcgacctggg caacgtccag gactacatcg agaccatgac ggacgccctg cggggcaact
     8761 tcgagtcggt cgaccggctc ctcggaccgc cgttcgatcc ggagcgtcac gtctggatcg
     8821 cccccgagtc gctggccatg cgcgactcga ccagcggggc cacgctggcg gagaagatcg
     8881 ccgacggctc ggtccggatc ggtccctccg tgcggatcgg acggttctgc gagatccacc
     8941 ccggggtgcg gatcgtggag tcgaacctcg acgacgacat cgaggtccac cgcgatgccg
     9001 ccatcgagcg cacccagatc cgcgacggcg ccatcgtggg ggccgcggcg cggatctccg
     9061 aggccgtggt gggctcgatg tccgaggtcc gatccgagcc ctacaaccag accatgatcg
     9121 aggatggggt ggcgctcggc gacgaggtga tcgtgtatcc gggcgtgcac ctgtccggcg
     9181 acgtatccgt ctacccgcgt ctcaagctcc catcgggg
//
LOCUS       DASQQQ010000250         3659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_215045, whole genome shotgun sequence.
ACCESSION   DASQQQ010000250 DASQQQ010000000
VERSION     DASQQQ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215045"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VE646_09495"
     CDS             <1..706
                     /locus_tag="VE646_09495"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZD80258.1"
                     /translation="QPQVDRIVRRLPQDRQTMFFSATLDGTVSRIADAYTRDARRHEI
                     QSQIRTVEEVEHRFLPVSAQRKVDALVELLEKDRDLTLVFVRTKRGADRLLAKLRSRG
                     IDVEAMHGDMTQPARQRALARFESGRVNVLVATDVAARGLDLDRISHVVNFDPPQDDK
                     DYVHRVGRTARAGRTGTGVTLVTAEQQGDVSRMAARLDLDDAFEAGGMKMAPPRVVFS
                     GSAGGRRSSLRRPRRR"
     gene            complement(848..1261)
                     /locus_tag="VE646_09500"
     CDS             complement(848..1261)
                     /locus_tag="VE646_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HZD80259.1"
                     /translation="MRVRRFVAPLVALLLITAMGCSGSSGDVTATEKDYSISLSKTDL
                     SSGEHTFDIKNDAGQTHEFVVIKTDLAEDQLPTDENGDVDEEGEGIEPVDEVEDIEGG
                     SSESLTVDLDPGTYVVICNLPGHYRQGMHETITVS"
     gene            1451..2116
                     /locus_tag="VE646_09505"
     CDS             1451..2116
                     /locus_tag="VE646_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD80260.1"
                     /translation="MPRLLVVDDEPRIVDFVSRALSSEGFQVDGASDGAQGLDLARTG
                     RYELVVLDLLLPERDGESVLRELMEQRPEQRVLVLSALSDVDRKVRCLELGASDYLPK
                     PFALAELVARIRARLRQPASGPDQRFLTVGGVTLDLTRRVAASGEAEAALSEREFLLL
                     RHLMMRGGDTCSRQRLLQDVWGYTFDPGSNVVDVCVGRLRAKLGSDVIETVRSVGYRF
                     AAS"
     gene            2088..3203
                     /locus_tag="VE646_09510"
     CDS             2088..3203
                     /locus_tag="VE646_09510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZD80261.1"
                     /translation="MWGIGSLLRRRWLEVAWGLFAAANVVVMLRLTSWETVPFHLIWV
                     SLTILYGYRVWSLGSTMALLAIVALVTGFALSRSVLGTHESWDEVTEVPLMSAMFMAM
                     VWHARRRLAAIERAERLARREHRMLEAQREFVRDASHVVRTPITVAHGHAELIRGSAV
                     DPQTRDDADVIVDELGKLSRLSEALLLLAAAEDRGFLRRRSVALEPFLAQMARRWLPA
                     ADRDWRIQPGATGSVQADPERLEIALDALIENAVKATAPGARISIACRRDGDELLFQV
                     SDAGVGIPADELPRIFERFARGEPSGWERAGTGLGLALVKAIVEAHGGDVGVQSRPGS
                     GTVFTIRLPAPERSEEVLPGGAGTVPALGGVPHTTSG"
     gene            3264..>3659
                     /locus_tag="VE646_09515"
     CDS             3264..>3659
                     /locus_tag="VE646_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014734202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZD80262.1"
                     /translation="MSRRMRALRKTRPGPGAELVEIPVPEPGEGEVLLRVHATSLCGT
                     DLHIYEWNEWAQGRIDRIPMTFGHELAGWVEAVGPEVHHLQPGTFVAAETHIACGVCS
                     PCRTGRAHICRNLRILGVDTDGAFAEYVVV"
BASE COUNT          488 a         1235 c         1342 g          594 t
ORIGIN      
        1 ccagccgcag gtggatcgca tcgtgcgccg gctcccccag gatcgtcaga cgatgttctt
       61 ctccgccacg ctggacggca cggtgtcgcg gatcgccgac gcctacacgc gagatgcgcg
      121 tcgacacgag atccagagcc agatccgcac cgtggaagag gtggagcacc ggttcctgcc
      181 cgtgtcggcc cagcggaagg tggacgcgct cgtggagctg ctggagaagg accgcgatct
      241 caccctggtc ttcgtccgca ccaagcgcgg cgccgatcgc ctgctggcca agctcaggag
      301 ccgggggatc gacgtggagg cgatgcacgg cgacatgacc cagcccgccc ggcagcgggc
      361 cctggcccgg ttcgagtccg gccgcgtcaa cgtcctggtg gccacggacg tggcggcccg
      421 gggtctggac ctggaccgga tcagccacgt ggtgaacttc gatcctccgc aggacgacaa
      481 ggactacgtc caccgggtgg gtcgcaccgc gcgtgccggg cgtaccggca ccggcgtgac
      541 cctggtcacc gcggagcagc agggcgacgt ctcccggatg gccgcccggc tggacctgga
      601 cgacgccttc gaggccgggg gcatgaagat ggcgccaccc cgggtcgtct tctcgggctc
      661 ggcggggggg cggcgctcgt ccctgcgtcg ccctcgccgg cggtgagggc atcggggatc
      721 agctcgccgg ctgatcggcc aggcgtgaac tgctgctcag gcgatcccac gggcggacgg
      781 cgtggaacgg aacaccggcg gtcgtcgccg gccgccggtg ccaggatcgg gtcttgcggg
      841 cgtggggtca gctcacggtg atcgtctcgt gcatcccctg gcggtagtgg ccggggaggt
      901 tgcagatcac cacgtaggtg ccggggtcga ggtccacggt caggctctcg ctgctgccgc
      961 cctcgatgtc ctcgacctcg tccacgggct cgataccttc gccttcctcg tcgacatcgc
     1021 cgttctcgtc ggtggggagc tgatcctccg ccaggtccgt cttgatgacg acgaactcgt
     1081 gcgtctgccc ggcgtcgttc ttgatgtcga acgtgtgctc gcccgaggac aggtccgtct
     1141 tgctgagcga gatcgagtaa tccttctctg tcgccgtgac gtccccgctg ctgccgctgc
     1201 accccatcgc cgttatcagc agcagcgcca cgagtggtgc cacgaaccgt cgtactcgca
     1261 tgggatcctc ctcctcgttc gggcccccat cccggaccac cgcagtgtag gcgggtcggc
     1321 tcgcgttggg gaccgtccgt gaccgatccg tcatccaacg gtcatgaggg tgtcaccgtc
     1381 gcgctcggcg ctcgtgacgg ttccgtcatc cgtctggtca gggaaggggc gcccgcctac
     1441 cattcccgcc atgccccggc tgctcgtggt ggacgacgaa ccgaggatcg tggacttcgt
     1501 ctcccgtgcc ctgtcttcgg aagggttcca ggtggatggt gcctcggacg gagcccaagg
     1561 cctcgatctg gcccggaccg gccggtacga actggtggtc ctggacctgc tgctgcccga
     1621 gcgcgacggc gagtcggtgc tccgagaact gatggagcag cgtcccgagc agcgggtgct
     1681 ggtgctctcg gccctgtccg acgtggaccg gaaggtccga tgcctggagc tcggtgcatc
     1741 ggactacctg cccaagccgt tcgccctggc cgagctggtg gccaggatcc gggccaggct
     1801 ccggcagccc gcctccgggc cggaccagcg gttcctcacg gtgggaggcg tgaccctcga
     1861 cctcacccgc cgggtggcgg cctccggtga ggccgaggcg gcgctgtccg agcgggagtt
     1921 cctcctgctc cggcacctga tgatgagggg aggcgacacg tgcagccggc agcggttgct
     1981 gcaggacgtc tggggctaca cgttcgatcc cggcagcaac gtcgtcgacg tctgtgtggg
     2041 acgattgcgg gccaagctcg gctcggacgt gatcgagacg gtgaggagtg tggggtatcg
     2101 gttcgctgct tcgtaggcga tggctcgagg tcgcctgggg gctcttcgcg gccgccaacg
     2161 tggtcgtgat gctgcggctg accagctggg agaccgtgcc cttccacctc atctgggtga
     2221 gcctgacgat cctctacggg taccgggtgt ggagcctcgg cagcacgatg gcgctgctcg
     2281 ccatcgttgc gttggtgacc ggcttcgcgc tgtcccggag cgtcctcggc acccacgaga
     2341 gctgggacga ggtcaccgag gtgccgctca tgtcggcgat gttcatggcg atggtgtggc
     2401 acgcgcggcg ccggttggcg gcgatcgagc gggccgaacg gctggcgcgc cgcgagcacc
     2461 gcatgctcga ggcgcagcgg gagttcgtcc gcgatgcgtc gcacgtggtg cggaccccca
     2521 tcaccgtggc ccacggtcac gccgaactga tccgagggtc cgccgtcgat ccgcagaccc
     2581 gggacgacgc ggacgtgatc gtggacgaac tgggcaagct gtcccgcctg tccgaagcgc
     2641 tcctgctcct cgccgcggcc gaggaccgag gtttcctccg ccggcgatcc gtggccctgg
     2701 agcccttcct ggcccagatg gcacgccggt ggctgcccgc cgccgatcgg gattggcgga
     2761 tccaacccgg ggcgacgggg tcggtgcagg ccgatccgga gcggctcgag atcgccctcg
     2821 acgcgctgat cgagaacgcg gtgaaggcga cggcgccggg agcgcggatc tcgatcgcat
     2881 gccgtcgcga tggggacgag ctcctgttcc aggtgtccga tgccggcgtg ggtatccccg
     2941 ccgacgagct cccccggatc ttcgagcggt tcgcgcgggg ggagccctcc ggctgggaac
     3001 gggcgggtac cgggctgggg ctggccctgg tgaaggcgat cgtggaagcg catggtggcg
     3061 atgtgggggt gcagagccgc cccggctccg gaaccgtctt cacgatccgc ctcccggccc
     3121 cggaacggtc ggaagaggtg ctgcccgggg gggcggggac cgttcccgcc ctgggtgggg
     3181 tcccccacac cacgagcggc tgaccctccg agggcccttg gctcctggtt cggcgccgcc
     3241 ggggctgcag taggctgcag gcgatgagcc gccgcatgcg ggctctccgc aagacgaggc
     3301 ccggcccggg cgccgagctg gtggagatcc ccgtcccgga gccgggcgag ggagaggtgc
     3361 tcctgcgggt gcacgccacg tcgctgtgcg ggaccgacct gcatatctac gaatggaacg
     3421 agtgggccca ggggcggatc gaccgtatcc ccatgacctt cgggcacgag ctggcgggct
     3481 gggtcgaggc cgtgggcccc gaggtccatc acctccagcc ggggacgttc gtcgcggccg
     3541 agacccacat cgcctgcggc gtgtgctcgc cctgccgaac cggccgggcg cacatctgcc
     3601 ggaacctcag gatcctcggc gtcgacacgg acggcgcgtt cgccgagtac gtcgtggtc
//
LOCUS       DASQQQ010000251         2087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_315977, whole genome shotgun sequence.
ACCESSION   DASQQQ010000251 DASQQQ010000000
VERSION     DASQQQ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315977"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /gene="mgtE"
                     /locus_tag="VE646_09520"
     CDS             complement(<1..1262)
                     /gene="mgtE"
                     /locus_tag="VE646_09520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HZD80263.1"
                     /translation="MSTPELQQVDEFQARLIRLEALLSSDSETELRAFLEDLHPSDIA
                     DLIESFEQEEDRVRLLDLLPAELASETLAEMEAHQHPEEILVALEPDRISELIAELSN
                     DDAADIIGGLTPEDQARVLATLAPVDAVELRELLAYPEESAGGIMTTELVAISIHLTA
                     GEAIQEVRRQAREIGGEFYNIFVVDLLRRLQGQVSLQDLVLNEPDRKLTDLVEPPVVT
                     VPVDMDQEEVGRLIARYNVPSVPVVGPNNVLLGRITWDDVMDVIEAEQTEDLLRLAGV
                     GAEEEVQGEWAEAVKSRLPWLVLNIVTASFGATVVILFESTIASMAILAGIMPVVAGL
                     GGNAGTQSLAVTVRRLALTREGRARRWRVAAKEVLVGLVNGAALGLIVGGVAHILFNV
                     LALGAGGAPPGYMLGVVVMLAMWANLIVA"
     gene            complement(1259..1999)
                     /locus_tag="VE646_09525"
     CDS             complement(1259..1999)
                     /locus_tag="VE646_09525"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HZD80264.1"
                     /translation="MNGLGKLLRRRLIAGLIVIAPLGVTAYILWWLFRAVDGILGNAV
                     YQLIGFPIPGLGLLLLLALLLGVGWLAERTVGSQILEWWHAALDRVPVVSRLYNATRR
                     IVGSVFGGEERRFFREVVLFEYPSAGQWTLGFVTGRAPRALAEKMSSGLTVFLPTAPN
                     PVTGFLIIVPEERIIRLPVTVEEGFTYILSAGAVPPMGVPGQAPDARAAAEAVRRSSP
                     DHHAGGPAVESRPLGAEQGPKRAGGGAP"
BASE COUNT          328 a          799 c          669 g          291 t
ORIGIN      
        1 gccacgatca ggttggccca catcgcgagc atcaccacga cgccgagcat gtagcccggc
       61 ggcgcccccc ccgcccccag cgcgagcacg ttgaagagga tgtgggccac gcccccgacg
      121 atcaggccca gcgccgcccc gttcaccagc ccgacgagca cctccttggc cgccaccctc
      181 catcggcggg cccggccctc gcgggtgagc gcgagccgtc gcaccgtgac cgccaggctc
      241 tgggtccccg cgttgccgcc gagccccgcg accacgggca tgatccccgc caggatggcc
      301 atggacgcga tcgtgctctc gaacaggatg accacggtcg ccccgaacga ggccgtcacg
      361 atgttgagca ccagccaggg cagccgactc tttacggcct ccgcccactc gccctggacc
      421 tcctcttcgg cacccacacc cgccagccga agcaggtcct ccgtctgctc cgcctcgatc
      481 acgtccatca cgtcgtccca ggtgatccgg cccaggagta cgttgttcgg accaaccacc
      541 ggaaccgagg ggacgttgta gcgcgcgatc agccgcccca cctcctcctg gtccatgtcc
      601 accgggacgg tgaccaccgg tggctccacc aggtccgtga gcttccggtc cggctcgttc
      661 agcaccaggt cctggagtga gacctgaccc tgcaggcggc gcagcaggtc gacgacgaag
      721 atgttgtaga actcgccgcc gatctcccgg gcctgccgac gcacctcctg gatcgcctcg
      781 cccgcggtca ggtggatcga gatcgccacc agctcggtgg tcatgatccc gccagccgac
      841 tcttccgggt acgcgagcag ctcccgcagc tcgacggcgt cgaccggggc cagggtggcc
      901 aggacccggg cctggtcctc cggggtgagc cccccgatga tgtcggccgc gtcgtcgttg
      961 ctgagctcgg cgatcagctc gctgatccgg tcgggctcca gcgccacgag gatctcctcg
     1021 ggatgctggt gcgcctccat ctccgcgagc gtttccgagg ccagctcggc ggggagcagg
     1081 tcgagcagcc ggacccggtc ctcctcctgc tcgaaggact cgatgaggtc ggcgatgtcg
     1141 ctcgggtgga ggtcctccag gaacgcgcgg agctcggtct ccgagtcgct cgaaagcagc
     1201 gcctccagcc ggatcaaccg cgcctggaac tcgtcgacct gctgcagctc gggcgtgctc
     1261 atggcgcccc tcctccggca cgcttcggcc cctgctcggc tcccagcggc cgactctcga
     1321 cggccggacc gcccgcgtgg tggtccggcg acgatcgacg caccgcctcc gcagccgccc
     1381 gtgcgtccgg cgcctgcccc ggcacgccca tcggcggcac cgcccccgcc gacaggatgt
     1441 aggtgaagcc ctcctcgacc gtgacgggga gccggatgat gcgctcttcg ggcacgatga
     1501 tcaggaaccc ggtcacgggg ttcggcgccg tcggcaggaa gacggtgaga ccgctgctca
     1561 tcttctccgc gagggcgcgg ggcgcccgac ccgtgacgaa tccgagcgtc cactgaccgg
     1621 cgctcggata ctcgaagagc accacctccc ggaagaaccg ccgctcctcg ccgccgaaca
     1681 cactgcccac gatccggcgg gtcgcgttgt agagacggga aaccacgggc acgcggtcga
     1741 gcgcagcgtg ccaccattcc agaatctgcg agccgacggt ccgctcggcg agccagccca
     1801 cccccaggag cagggcgaga agcaggagga gaccgaggcc cgggatcggg aacccgatca
     1861 gctggtacac cgcgttgccg aggatcccgt cgacggcgcg gaagagccac cagaggatat
     1921 aggcggtgac gccgagcggg gcgatgacga tcaggcccgc gatcagccgg cggcgcaaga
     1981 gctttccgag accgttcatc cgggccgcac ctcccccagg atccgctcct ggtgatgcca
     2041 catctcgctc ccctcccgct cctcctccgg atgatcccag ccgagca
//
LOCUS       DASQQQ010000252        26332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_15404, whole genome shotgun sequence.
ACCESSION   DASQQQ010000252 DASQQQ010000000
VERSION     DASQQQ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 26332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26332
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15404"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VE646_09530"
     CDS             <1..330
                     /locus_tag="VE646_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80265.1"
                     /translation="TAASAYLRADPRGHPTIALEGRIVRGRCRASNPRMALLCRLMLR
                     SRERGAQMLIEVTCMCGFQARGTEDDVVAQMQAHGLADHGQASSRETILRMAIPIGEG
                     PEDGRLG"
     gene            314..877
                     /locus_tag="VE646_09535"
     CDS             314..877
                     /locus_tag="VE646_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80266.1"
                     /translation="MAGSAESDRPYVELFVPALTNVSWSTEIVGQTLEALFVCLRPDG
                     AHRLRPIHAPALRLGWRPDLQPADVILGAVRRYGLRPLLLHSTSWRFEGARVFLTYVA
                     AVAPPDELDQYLVDEPVRRSDLARGDAMGGTPEIGLAQVVEHAFRHLSWLVKDDAAVR
                     EALSDWVGFLEAYEPEPFRAFGPGLPT"
     gene            complement(888..1640)
                     /locus_tag="VE646_09540"
     CDS             complement(888..1640)
                     /locus_tag="VE646_09540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZD80267.1"
                     /translation="MTQDHVLRNRTAWDGFAEEYVEPGHQAWASDEPTWGIWGVPEAD
                     VGMLEDVEGLDAVELGCGTGYISVWLARRGARPVGLDNSPKQLETARRFQDEFDLRFP
                     LIHGDAERTPFADESFDLAISEYGAAIWCDPYAWIPEAARILRPGGRLMFLGHTPLVT
                     ICAPEDEDAPLEERLLRDHFGMHRLEWSDSVEFQLPHGEMIRLLRSCGLEVEDLIEVR
                     APEGATSRLDWVTPEWARRWPTEDVWKARKRR"
     gene            1722..1943
                     /locus_tag="VE646_09545"
     CDS             1722..1943
                     /locus_tag="VE646_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80268.1"
                     /translation="MAAGSKFTKSKSNSGTEKEGPGLLLSDARFQVPKLATKRRILEM
                     IGKTEAFGIQTFDAIMTPEPAPPITAENR"
     gene            1975..2223
                     /locus_tag="VE646_09550"
     CDS             1975..2223
                     /locus_tag="VE646_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80269.1"
                     /translation="MKTTRKRIRDESLLGFFFGATEREYDMARTLGDRYLFAFIVLND
                     TNDYGRSFAVLLPLDEVERRTQKRRIQYQVNFGAISPP"
     gene            complement(2294..2653)
                     /locus_tag="VE646_09555"
     CDS             complement(2294..2653)
                     /locus_tag="VE646_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80270.1"
                     /translation="MPIFRVLATRTEFYSIEAFFRAPNRDEAEEVFHESLEGHPLSLR
                     WDQDYDGSDTEIETVEDVTEQHDPDPEGVERSVCRLCGRPVRWTGVPAEPSPTGRTIP
                     GPWIHLLNPLVEQGIGL"
     gene            complement(3456..4133)
                     /locus_tag="VE646_09560"
     CDS             complement(3456..4133)
                     /locus_tag="VE646_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80271.1"
                     /translation="MRIADEDDELRAAAFAYLSELTARTGGLVKRSDLRQFTFRGQRI
                     SLEQNMRGIRVVRGHTAALSILTTFRERPEDRPYDDNIGRDGYPRYKWRGADPHFRDN
                     DALRAAKDLRKPLAWSAGVSPGLYEVVFPLWLIDEERSEQQFVVALDPIMDANWTRDV
                     LLTGPDEIRRREYAMTVVHGRLHQPDFRRRVLAAYGANAPCVVSAIRNCCMPPTSKRI
                     LRAASRW"
     gene            4224..4631
                     /locus_tag="VE646_09565"
     CDS             4224..4631
                     /locus_tag="VE646_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80272.1"
                     /translation="MSSQNEGGARTALERELYWAEEATPRSRMDISEVRDALHDFAVL
                     MRTDETQMIPRGEPNLAARKRWKRQLKLRLFRVMRPISWRYDRLLADLGELTAALADR
                     VATLEAEVSRLRTERQPEREPEPPRPDVSEPTS"
     gene            4628..5677
                     /locus_tag="VE646_09570"
     CDS             4628..5677
                     /locus_tag="VE646_09570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZD80273.1"
                     /translation="MRIAVCHPQSPFVRGGAETHTEAVVRALKAAGHEAEMVTVAGKW
                     YPATELVHQMAVWRSIDLSESNGLKIDAVIALKFPAYLVQHERKIVWLIHQHRSAYEL
                     WDHPEFADLSKQDEGPIVRDMVWQSDRVSLGEAKRIFTNSKNVQDRLWNTLRLSSEVL
                     YHPSPITEALLQREPGPYGDAIVFPSRLEALKRQPLVVEAMRHVKTDVRLVLVGSGPD
                     EPAIREQIAKLGLGDRVSLQIGLPDEPLHRLFLGALGVYYGPFDEDYGYVTLEGFAAH
                     RPVVTLTDSGGPLEFVEQDATGLVVPPEPTSIAQAFDRLYADRALAKRLGEAGHETIR
                     ATVPSWPGVVARLLD"
     gene            5682..7064
                     /locus_tag="VE646_09575"
     CDS             5682..7064
                     /locus_tag="VE646_09575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZD80274.1"
                     /translation="MGNPVVGAARRLFRWDYQMDPRIAWLSPMPPAPTGIATYSKAVL
                     EGLDRIRFAREHHRIQPVWPIKPSHEGAIPWHTMAVYHLGNNVEFHRDIYRHAIQTPG
                     LVVLHDLALDDFVRGLVTGGDPLGHQARREALANGPRLEGFEDALRNEPLRLPWAAHV
                     ARRARGIVVHAPFCERYLRAFGCRTPVYVAPHPVLERDEDVRRAERRRAVLRAPLQVQ
                     GMRTLVGAFGDLNAAKLLDVLCEAVRRLPEDVHLVLVGRRIEGYDVDAVVGASGLGGR
                     VTLLPDVSDADFLGWLCAVDVAVDLRFPHRGEVSGSLARAMQCGRPTVVSATGTYLDV
                     PDEFVLRAPPGRPDPDAVTAVLRRLIEDEGLRERMGAAARAHTAEQARGDRTAHVYSA
                     AIEGTLALLRDPGRRALARWAGALSDLGVTEEHLGRDYGVSYARALDEFEPGAGPVGS
                     EAPTAGEGPS"
     gene            7103..7825
                     /locus_tag="VE646_09580"
     CDS             7103..7825
                     /locus_tag="VE646_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80275.1"
                     /translation="MLGAGRRLGSRAAYEAKTLVAASPRLTVAAARWRGHGEVVGPGS
                     DILIEGYPRSANQFSVAAFRLAQGRPMRVAHHTHAPGHVLAALRLHVPAIVLIRDPCD
                     SVLEFVLVRPTLTVRQALRGWIRFYSALQPHRARFVTGPFSEVTSDFGAVIRRANERF
                     GSAFVPFDHTEENVRECFRQMDAYWEGRIGATEELERYVGRPSATRDRWKDDLLETYL
                     RLPSALRSGAEELYRNFAGGAS"
     gene            7822..8595
                     /locus_tag="VE646_09585"
     CDS             7822..8595
                     /locus_tag="VE646_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80276.1"
                     /translation="MTAQRSSAANLGSLAAEGKRLLRRGAYEAKSLVARYPALALPIA
                     RRRHGVPVDERTRIVIEGFPRTGTSFAVAAFDLAQDGGVPIACHVHAPAQILEGIRRG
                     LPVVAVVREPEDTVLSFMIRNPHLEASTSLRGYLRFYEPLRPHLGRIVVGTFPQVTAD
                     MGAVIRRVNERFGTGFASFEPTEDNVRRCFEAIEADYRKRLPEGEALEREVARPSAWR
                     RERKEELRAAYRSPSTARLRARAERVFQAFADAAGTGDP"
     gene            8592..8969
                     /locus_tag="VE646_09590"
     CDS             8592..8969
                     /locus_tag="VE646_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80277.1"
                     /translation="MTRILIETLLAAQPVDPDRHLDHARMQRFTAILDELPPVVVFDT
                     PEGMLLADGYHRLAAARRLGRDSLEAEIKRGTRHDALRWAAQNAAAERGISEGEALGH
                     IERHRGGRRPDSKGAGEVGRGSR"
     gene            8956..9741
                     /locus_tag="VE646_09595"
     CDS             8956..9741
                     /locus_tag="VE646_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80278.1"
                     /translation="MDRGSSAGRLAARSIYEAGTLVGRWPSVALPVARWRGHGEPLDT
                     HTELVVEGFPRSSNSLTVAMIRAAQPEGIRIAHHVHAPSHVLESVRRRLPTLVMIREP
                     EDAVVELALLKPAITVAQALRGWVRFYGPLEGHRREFVVARAEEIPLHVGRVVDRLNE
                     RFGTDLLPPDVGPEDLGRAEADTEGYWTGRSGPGLPLIGRTEARDVGAVGGAGQAGEG
                     ADAADQAERRALHRAYAERRLTRLRSRAERLHETLTGGLPDLG"
     gene            9810..10727
                     /locus_tag="VE646_09600"
     CDS             9810..10727
                     /locus_tag="VE646_09600"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD80279.1"
                     /translation="MAADAVTAVTVRTSEPPRLRMIDRMRAVHSHREILMNLVRKELK
                     VKYAHSLLGAIWSLLNPLVFLAVFTFVSKVLGSAIPHYAVYLLSGLLAWNLFSVSLLT
                     GARSVVDNANLVKKVAFPREILPLSSVGVALVDYVLQMAVLLLFIVASGYGFHVPELA
                     LFPLAFVTVLVFTVAMTFWMAALNVRYRDVQHLLNVGLLVWFWATPIVYGSWQVQNML
                     GNRAIWGMSLFDLYFVNPLTPITLALQRSLYAFVSPLSGGQPVPVLAHVSLPWSAIVT
                     GLVLVGSMLLLRLTWGTFFHLSGDFAEEL"
     gene            10724..11905
                     /locus_tag="VE646_09605"
     CDS             10724..11905
                     /locus_tag="VE646_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD80280.1"
                     /translation="MTAAIEVDHVSKKFRLFRERPTSIKQRILTSRSRAEDFWALRDI
                     TFDIPEGSTLGLIGHNGSGKTTLLKIVAGILRPTAGTVRQRGRLAALLELGAGFHPEL
                     TGKENVYLNASFLGLSRRETDRAYDGIVDFAELEGFMDNMVKFYSSGMLVRLGFAVAV
                     HVDPEVLLIDEVLAVGDEAFQAKCLDRIGRFQREGRTIVLVTHALDTVRSICHRAAML
                     HHGSLHAIGAPDDVVREMRYVLLGVTDPRFVPEEGTREAEIASVEIVRADGKSEGPVL
                     KGDPLTIQVDVRTNQPVDDLDVDFQVLDGATNHAVLGARTSQDGSPIERFDGKKRVLF
                     RIGAFPYTAGKYWVTIGLSSRETGKLYHVQTQRYLFEVIEAPRVQEVLDVPVQSVVED
                     L"
     gene            11990..12361
                     /locus_tag="VE646_09610"
     CDS             11990..12361
                     /locus_tag="VE646_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80281.1"
                     /translation="MLIVLILVSVTLAAVAQLTLKHGMNQVTARTGVLELTGESARSV
                     VITPAVWIGLVLFGLSAVVWLGVLSRTSLSFAYPFAAITYVLILLFDKLVLDEAVPPL
                     RWAGVAFIAIGIFLVSRTPSG"
     gene            12363..13232
                     /locus_tag="VE646_09615"
     CDS             12363..13232
                     /locus_tag="VE646_09615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZD80282.1"
                     /translation="MAGPPHAELAVVIVNYDAGEYLERCLASLERHRGDLEVDVLVID
                     NASHDGSPLAARSAHPWVRLIENPVNRFLSPAWNQGAAETRAPFLLFLNPDTEWWQGT
                     LEDYVEEARRHPNAGIVGPVVRNLDGTVYPSGRVFPAVVDAVAHAFLGTLMPRNRFTR
                     RYQLEGWDRSTERDVDWVSGCCMLMPRKAFEAVGGFDESFLLYGEELDIATRLRRSGW
                     SVLFTPRVEVVHAIGVSTGRSRAMLVRHSQSIYRYYRKHRAPGWRRLTLPLAWVVLRL
                     RAEVEWLRAGPRS"
     gene            13229..14260
                     /locus_tag="VE646_09620"
     CDS             13229..14260
                     /locus_tag="VE646_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HZD80283.1"
                     /translation="MKAIVLVGGEGTRLRPLTETIPKPLLPLMDRPSLDHVLDHLARH
                     GVHEVVLSSSYLESTFHAFIESRRGDPSITWITEEAPLGTGGAVINALGTVDDEPVFA
                     LNGDILTDLDLTAMLAFHRQRGAAATIALHRVEDARPFGLVLTEPEGRVLEFREKPQE
                     LVPGEINAGTYVLDPRVIRDWPTGRAISIEREIFPELIRAGRPLFGFASDAYWLDLGT
                     PEKYLQAHFDILEGRVGFEPPFPSPYVAEDAEIDLRSHLGRWVVIGTGASVGPDAQVD
                     DSVLHPGARVEAGARVVGSILGPSSVVGAGATVIDSALAERARVPPGLPARGLRLSAG
                     REAAAPEGA"
     gene            14318..15838
                     /locus_tag="VE646_09625"
     CDS             14318..15838
                     /locus_tag="VE646_09625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HZD80284.1"
                     /translation="MIRRALIAALSLALLLAPAVPAGAADSFTFFGSGWGHGLGLSQW
                     GAYGLSEQGWGTGRILTHFYSGTRVTSEAQPPRLVRVGLAQGRARFRLSALGGAVDLR
                     LGDRDPASTIATIPDGETWTVRLVQGSYRLIDATGTAVGDPVGGTAMNLYAVFEPNGA
                     VLKVPETGHTYAHGWLEFNIYGCATTCVMRLVLAVAPQPYLYGIGEVPSSWPTASLRA
                     QAIAARTYAFRKIRSTGQHRPGCNCALFASPIDQVYVGYDKEAGYLGDRWVAAVDATK
                     DRVVTYRGTLIEAYYSASSGGFTENNENVWGGTPVPYLRGVCDPGDYTIANPNAVWKT
                     VLTAKQVTARLGLGIGRVTGFGETVRGVSGRIVSVIVKGTTGRASVAGGTLRAALGLP
                     DDRVWINHNRQVTGTIRQKYDAIDCRSGLPTSRRVRVAGGARQVFQLGTIFAKDGIGA
                     HEVRGTVLAFYRKHGGPRGSLGFPITDTHRLKNGNTRSRFEHGTVTCRPSGSCWRS"
     gene            complement(15860..16600)
                     /locus_tag="VE646_09630"
     CDS             complement(15860..16600)
                     /locus_tag="VE646_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80285.1"
                     /translation="MAKKRRRSRGSRPPGGGTATAVEEGARSRRAEHKELARQRREQE
                     RKRAKRRRFVRTYLILVGISAVSVSIIILVMGPRSTPSGPLPGLLKDTAPWPANTAQV
                     GARLDRLDLPAAGGAEHIHVDLQIFVKGSAEPVPADIGLASGVESPLHTHESGGVFHV
                     ESATQRTFTVGNVFDVWGVRLTRTCLGGYCDSGAERIRAYVNGSPITGSPRDVALADH
                     DVVVLTFGTSAQEPTPIPSTYDWSTLNP"
     gene            complement(16606..17316)
                     /locus_tag="VE646_09635"
     CDS             complement(16606..17316)
                     /locus_tag="VE646_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80286.1"
                     /translation="MAKQGTNRGTNQGRMTKAERREQARRERLELQRKMARTRRNRRL
                     AVIAAVVIVGAAVVAVVSTRSPSSTATPGQLAGVLDSKAPWPANTQDLGQRLRVLGLP
                     PEGGALHHHAFLAISVDGSPVTVPADIGLAVSTASPLHTHDTTGVIHIESGNPSFSPT
                     LGEFFDVWGVRLTSSCLGGYCTEGDQQLRVFLNGRPVQSNPRSVPLKEHDDVVVAFGT
                     AAQVPQPLPTFDWSSFQG"
     gene            17476..18882
                     /locus_tag="VE646_09640"
     CDS             17476..18882
                     /locus_tag="VE646_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HZD80287.1"
                     /translation="MVGTRERRAAGLDGLFHITERGSTVRTEIVAGLATWLTMAYILF
                     VNPAILGSVQDHTGTSLPFGQVLTVTALTAGVMTILMGVVGNYPFAMAAGLGLNAFVA
                     FTLVSGYGLSWPDAMGVIVIEGLVIAALVLTGFREAVLNAIPMDLKRAIGIGIGLFIA
                     FIGLVNAGVVIHPRGGTIVTLNPDLTTLRMLTFVIGLGLTAGLVARKMRGALLIGIVA
                     TTVVAIAINEIWGHSRIWQTVGPGVAQIPSRIVESPDFSLLGNFSIAGVFDALPFFTA
                     IAVILSVMLSDFFDTMGTVIGIGGEGKLLDREGRLPGIGKVLFIDSLGAAAGGAASAS
                     SNTTYIESAAGVSEGGRTGLTSVVVGILFLLAVFLSPLAEVIPPEATAPVLVIVGYFM
                     MTIVRDIEWSDPTLGIPALLTIIVMPFTYSITNGVGAGFVSYTVIELLRGRWRKVHPL
                     LYVVSAIFVWYFVRGLLA"
     gene            complement(18979..20268)
                     /locus_tag="VE646_09645"
     CDS             complement(18979..20268)
                     /locus_tag="VE646_09645"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007264464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HZD80288.1"
                     /translation="MSVEVLPVPGIPEVRPGDDLIVVLTPALRAVGLRDGDVIAVTQK
                     IVSKAEGRVVPEEGGGRAAWVERETRRVVARREELVVAETRHGFVCANAGVDASNVEP
                     GLLTLLPEDPDRSARRLREGISAAFGAPVGVVITDTFGRAWRRGLLNVAIGCAGLPAL
                     VDLRGRPDHTGRILEATVVALADEVAAASGLVMGKADRVPVAVVRGIHAEAEDGEAAD
                     LIRPPEEDLFRESPLVSISARRTIRSFGPGDVARPALEEAVRAACTAPAPHHTRPWSF
                     TVLASAPSRRALLGAIAEAWRADLRGDGTPEDTIARRIARSDAVLGGAPVLIVPWVRF
                     RGAHPYPDRERATAEREMFLLSGGAAIQNLLLALHAQGLASCWISSTLFCQEETRAVL
                     GMGDEWRALGTVAVGPMPDGRPAPRAEIDLGHLADFR"
     gene            complement(20276..21229)
                     /gene="cofD"
                     /locus_tag="VE646_09650"
     CDS             complement(20276..21229)
                     /gene="cofD"
                     /locus_tag="VE646_09650"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HZD80289.1"
                     /translation="MELVALAGGVGAGKLLRGLQRLAFDEGHGLTVIVNTADDIELWG
                     LHVSPDLDSVVYWLAGGMDRERGWGRGDETFRVRDELEAQNMLPTWFALGDRDLATHL
                     YRTELLREGWTLTAAAAAVVEAFELPWTVLPMSDDPVTTMVQIQEPDALRHDVHFQEY
                     WVRRGAEPPVRAVRFEGAIRARPAPGVLEAIRTADAVVICPSNPVVSIGPILAVPGIR
                     DALWERSDRVVGISPIVGGTVLAGMADKLMPVVGLEVSALGAAKAYEGLLTGWVVDGA
                     DRDLAPKIEERLGIRVAVTDTIMRDDRVTTELARTALDLLR"
     gene            21258..23759
                     /locus_tag="VE646_09655"
     CDS             21258..23759
                     /locus_tag="VE646_09655"
                     /EC_number="2.5.1.147"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF006687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional FO biosynthesis protein CofGH"
                     /protein_id="HZD80290.1"
                     /translation="MADAISHQTRRALARAEAGKALSVPEAEALLSARGPELRRLMEV
                     AARLRDLGHGSVVTYSPKVFIPLTMLCRDHCHYCTFAKPPAHLEQPFLSPEEVVAIAR
                     AGRDLGCKEALFTLGDKPEDRYPVARRWLEERGYGTTLEYVRAVAIKVIEESGLLPHL
                     NPGVMSWEDMARLKHVSASMGLMLETASERLSGRGMAHFNSPDKVPAVRLRTIEDAGR
                     LAVPFTTGILVGIGETARERAESLFAIRELHRRYRHVQEVIVQNFRAKPRTGMHASPE
                     PSEEEFLAAVATARVVLGPAMSVQAPPNLSDPDQQLRLLDAGINDWGGVSPLTPDHVN
                     PERPWPQIESLAARTAERGFELRERLTIYPGFALRPDPYLAGKMRAPVVALMGPDGLA
                     APHRQPEPIAWQDPDVAWKPRSTAPTFAKAPEAGLREDAAAVYGDLEALENEVTRRWA
                     RREVFPERIDALTKRALSRAEAHRPITDDEALALFQAEGPALEALCRVADDLRGQAVG
                     DEVTYVVNRNINFTNVCYVGCRFCAFAQREADAESYTLTLQEVADRAREAAEWGATEV
                     CMQGGIHPDLPGTFYFDLLDAVKAAAPDIHIHAFSPMEILNGSSKLGITFREFLTECR
                     SHGLGTIPGTAAEILDDDVRWVLTKGKLPADTWEQIVRTAHELGIRSSSTIMYGHVDA
                     PPHWVFHLRRLGAIQRDTGGFTEFVPLTFVHQNAPIYLAGKARPGATFAENRRMHAVA
                     RILLDGAIDNIQVSWVKMGTEACQLILQGGANDFGGTLMEETISRMAGAQWGIRMEPS
                     EFDAAIRAIGRIPAERTTTYERLRRNHSADIPVAG"
     gene            24024..24239
                     /locus_tag="VE646_09660"
                     /pseudo
     CDS             24024..24239
                     /locus_tag="VE646_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015772132.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
     gene            24259..24384
                     /locus_tag="VE646_09665"
     CDS             24259..24384
                     /locus_tag="VE646_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80291.1"
                     /translation="MSPAKKRSTAKKKTTAKKKTAARKPAAKKTAARKRTTAKKR"
     gene            24540..>26332
                     /locus_tag="VE646_09670"
     CDS             24540..>26332
                     /locus_tag="VE646_09670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZD80292.1"
                     /translation="MPSVLAVLVVKDGLPWLRECLQSLSGQSYARFGVLAVDNGSQDG
                     SVDVLARALGPGRVLRLSEDRGVAGALRAALELPAAQAADYLLLVHDDTALAEDAVTR
                     MVEAAEGIRGVERVGVVGPKVVDWDHPRVLREVGRSTDRFGHPDTPLQEGELDHGQYD
                     RVLEVLYVPTCAMLISRAAWQRTGLLDERLAGHHDDLDFCWRARVAGFRVLMTPLARA
                     RHVAAGTHGERPGAPARRHSDGYQAERAALASMLKNYGVLSLVWLLPLHALFGLIRLV
                     YLSLSRRFEEVFDLLSAWMWNVLHLPSTLRRRVRAQSVRSTGDRAVRRFMQSAWFRVP
                     RWFQTAELLLEEQAQEEREQVRIRARAASLATEHPVLVTWVLAIVVGALAFRELIGPG
                     ALQGGVLAGFPGHPATFFRELVSAVRTTQLGGTQAASPALAPLGALSAVLFASPALAQ
                     KVLLVGLPVLAGLMMYRSVHRQTQHAVAACVAAGAYTLSAMTFWAFSEGRIDVLVALA
                     AFPAIADRLEAAFASDGPAVRWRAAVGLGMTVAVAVAFLPGIVVALVPLFAVHVIGGR
                     RRIRGTGLALLGAAVAAVLVFPTLPGWAGDPG"
BASE COUNT         3751 a         9288 c         9262 g         4031 t
ORIGIN      
        1 acggcagcat ccgcctacct gcgagctgat ccccggggtc accccacgat cgccctcgag
       61 ggccgcatcg ttcgaggccg ttgccgcgcc tcgaaccccc ggatggcgct cctgtgtcgg
      121 ctgatgctac ggtcccgcga gcgaggagcg cagatgctga tcgaggtcac gtgcatgtgc
      181 gggttccagg ctcgtgggac cgaggacgat gtggttgccc agatgcaggc acacggcttg
      241 gccgatcacg gtcaggcgtc ctcgcgcgag acgatcctcc ggatggcgat tccgatcggg
      301 gagggtccgg aggatggccg gctcggctga gtccgaccgg ccgtacgtcg agctcttcgt
      361 ccccgcactc acgaacgtgt cctggtcgac cgagatcgtc ggccagaccc tcgaagctct
      421 gttcgtctgt ctacgaccgg atggcgccca ccgcctgcgg ccgatccacg cgccggccct
      481 gcgcctcggg tggcgcccgg acctgcagcc ggccgacgtg atcctcgggg ccgtccggcg
      541 gtacgggctc cggccgctcc tgttgcactc gacctcgtgg cggttcgagg gagcccgcgt
      601 gttcctcacg tacgtcgcgg ccgtcgctcc tcccgacgag ctcgatcagt acctcgtgga
      661 cgagcccgtc cgtcgctccg atctggctcg gggagatgcg atgggcggga cgccggagat
      721 cgggctcgcg caggtcgtcg agcacgcctt ccgtcacctg tcgtggctgg tgaaggacga
      781 cgctgccgtg cgagaggctt tgtcggactg ggtggggttc ctggaggcgt acgagccgga
      841 gccgttccgg gccttcgggc ccggcctacc gacctgagga tccagtctca gcggcgcttg
      901 cgggccttcc agacgtcctc ggtgggccag cgccgagccc actcgggggt gacccagtcc
      961 aaccgcgagg tggcgccctc gggggcgcgg acctcgatca gatcctcgac ctcgagcccg
     1021 cacgagcgga gcaggcggat catctctccg tgcgggagtt ggaactcgac cgagtccgac
     1081 cactccagcc ggtgcatccc gaagtggtcg cggagcaggc gctcctcgag cggggcgtcc
     1141 tcgtcctcgg gcgcgcagat cgtgacgagg ggcgtgtgcc cgaggaacat gaggcgcccg
     1201 cccggccgca ggatgcgcgc ggcctccggg atccaggcgt acgggtcaca ccagatcgcg
     1261 gcgccgtact cggagatcgc gaggtcgaag ctctcgtcgg cgaacggcgt tcgctcggca
     1321 tccccgtgga tcagcgggaa gcgcaggtcg aactcgtcct ggaaccgccg ggccgtctcg
     1381 agctgcttcg gcgagttgtc gagcccgacc ggcctggccc cgcgccgggc gagccagacc
     1441 gagatgtagc cggtcccgca cccgagctcg accgcgtcca gcccctcgac gtcctcgagc
     1501 atgccgacgt cggcctccgg cacgccccag atgccccacg tcggctcgtc gctcgcccag
     1561 gcctggtgcc ccggctcgac gtactcctct gcgaagccat cccaggccgt ccggttcctg
     1621 agcacgtggt cctgcgtcac gggcaggacg atacctggac gagacccaag cggatcgagt
     1681 gagtggttga tccctccggc cggccacagg atcatgggtg tgtggctgct gggtcgaagt
     1741 tcaccaagag caagtcgaac agtgggaccg agaaagaggg ccctgggctg ctgctctcgg
     1801 atgcgcgctt ccaggtgccc aagctggcca cgaagcgacg catactcgag atgatcggca
     1861 agacggaggc gttcggcatc cagaccttcg acgccatcat gacgccggag ccggcgcccc
     1921 cgattaccgc cgaaaaccgt tgacggcttg ttccccacgc tgcgactcat cgagatgaag
     1981 acgacgcgaa agaggatccg tgacgaatcg ctcctcgggt tcttcttcgg tgcgaccgag
     2041 cgtgagtacg acatggccag gacactcggc gaccggtacc tgttcgcttt catcgtccta
     2101 aacgacacga acgattacgg gcggtccttt gccgtcctgc ttcccctcga tgaggtcgaa
     2161 cgacgaacac agaagcgtcg gatccagtac caagtcaatt tcggagcgat ctcacctccg
     2221 taggaggtcc gaagaagggc tgggtgctct tgttcggcga cgagtccgac ctgccgacga
     2281 tcgagccgct cagctagagg ccgatccctt gctcgaccaa cggattcagg agatggatcc
     2341 atggacccgg gatcgtgcgc ccggttggcg agggttctgc cggaacccca gtccagcgaa
     2401 ccggccttcc gcatagccga cagacactac gttcgacccc ttcgggatcc ggatcgtgtt
     2461 gctcggtgac atcctcgacg gtctcgatct ccgtgtcgga gccgtcgtag tcctgatccc
     2521 aacggagcga gagaggatgt ccctcgaggc tctcgtggaa gacctcttct gcttcgtccc
     2581 ggtttggcgc acggaagaac gcttcgatcg agtagaactc ggtgcgggtg gcgaggacac
     2641 ggaagatcgg cacagctctt acctcacgac cttcgcgagc tgggcgactg cctcttcctc
     2701 ctcgtctccc tgcgtgtacg tggtggagat cagccacggg gttcccacga cgtttctggg
     2761 gtggttcctg tgacggtgag gtcaacaccc attcctcggc ctgctgagcg ctactcagca
     2821 gcaccgcctc ggggtctgga tatccatcgc tcgtccagaa cgtgccgacc catacctcgc
     2881 tcggctcctc gtgaagagag gtgtcgacga gacgaacgga gaagtccccc acgtcccacg
     2941 accaggaaac agcgactcga tggcgcgtcc acgctcgttc gagagcagcc aggcccaccg
     3001 ttccgtctcc cgctcggcgg tccgctcctc cgcatagcgc tcgggcagaa accgcgtggc
     3061 cataccctcc ataacgagga gagcccagaa ccttgggagc gcagaacgac gcatgggtct
     3121 gtctatcgcg ggcgagcctt caacgtctgt gaagtccgtc acagtccggg gacgctatcg
     3181 atccgaagtc agatcgtcag atcccttggc agactcaacc cgcatttcgg aagcgtgcat
     3241 accgctcttc aagcaagtcg gggtccggtc ggtgtgagcg ccgtcttgga acccagagct
     3301 tcctcccctg gatgccctgc agggcatgtt gaagcgtggg gccgtcgtgt tcagcgagaa
     3361 cgtcgcgccg gacttcgact tgatgccggg gctgacgccg acaacgaacg catcgaacgc
     3421 tcgatggtga attgcgcaca tggcaatccc gttcgtcacc accggctggc cgccctcaga
     3481 atcctctttg atgtgggcgg catacagcaa ttccggatgg cggagacgac acagggcgca
     3541 ttggcgccgt acgcagcaag cacacgtcgt cggaaatcgg gttggtgaag ccgtccgtgt
     3601 accaccgtca tggcgtactc gcggcgcctg atttcatcgg gccctgtgag tagcacgtcc
     3661 cttgtccagt tcgcatccat gatcggatcg agagcaacga cgaactgttg ttcactacgt
     3721 tcttcatcga tcagccacag ggggaagacc acttcgtaga gtccggggct gactcccgca
     3781 gaccacgcga ggggctttcg aagatcctta gctgctcgca aggcgtcgtt gtctcggaag
     3841 tgagggtccg ctcctcgcca tttgtaccgc gggtacccat cccgaccgat gttgtcgtca
     3901 tacggccggt cttcagggcg ctcccgaaag gtcgtcagaa tcgacagcgc ggcggtatgg
     3961 ccccgcacga cgcggattcc gcgcatgttc tgctcgaggg agattcgctg tccgcggaag
     4021 gtgaactgtc gcaagtcgga gcgcttgacc aggccccctg tgcgggccgt gagctcggag
     4081 agataggcga acgctgccgc tctgagctcg tcatcttcat cggcaatccg cacgcgcgaa
     4141 gtatgccgcg actacccagg cggctcaatc gcgtccaaga gggaacgatc gccatcaccc
     4201 ccggtgctac cctcaacggg ccgatgagca gccagaacga gggaggggcg aggaccgccc
     4261 tggaacggga gctgtactgg gcggaagagg ccacgccgcg ctcgcggatg gacatctccg
     4321 aggtccggga cgccctgcac gacttcgcgg tgctgatgcg caccgacgag actcagatga
     4381 tcccccgggg tgagccgaac ctggccgcgc gcaagcggtg gaagcgccag ctcaagctcc
     4441 gcctcttccg ggtgatgcgc ccgatcagtt ggcggtacga ccgactcctg gccgaccttg
     4501 gtgagctgac cgcggccctg gcggatcggg tcgcgaccct cgaggccgag gtttcccgcc
     4561 tccgcacgga gcgccagccc gagcgggagc ccgagcctcc tcgacccgac gtctcggagc
     4621 ccacctcgtg aggatcgcgg tctgccatcc gcagtccccc ttcgtccgcg ggggggccga
     4681 gacccacacc gaggccgtcg tccgcgccct gaaggccgcc ggccacgagg ccgagatggt
     4741 cacggtcgcc ggcaagtggt acccggccac cgagctggtg caccagatgg ccgtgtggcg
     4801 ctcgatcgac ctctccgagt ccaacggcct gaagatcgac gcggtcatcg ccctcaagtt
     4861 ccccgcctac ctggtgcagc acgagcggaa gatcgtctgg ttgatccacc agcaccgctc
     4921 ggcctacgag ctgtgggacc accccgagtt cgccgacctc tcgaagcagg acgaggggcc
     4981 gatcgtccgc gacatggtgt ggcagtcgga ccgggtctcg ctcggcgagg ccaagcgcat
     5041 cttcacgaac tcgaagaacg tccaggatcg cctgtggaac acgctccggc tgtcctcgga
     5101 ggtcctgtac cacccctcgc ccatcaccga ggcgctcctc caacgcgagc ccgggcccta
     5161 cggcgacgcg atcgtgttcc ccagccggct cgaggcgctg aagcgccagc cgctggtggt
     5221 cgaggcgatg cgccacgtga agaccgacgt ccgcctggtg ctggtcggct cggggcccga
     5281 cgagccggcg atccgcgagc agatcgcgaa gctcgggctc ggcgaccggg tctcgctcca
     5341 gatcggcctg cccgacgagc ccctccaccg cctgttcctg ggcgccctcg gcgtctacta
     5401 cgggccgttc gacgaggact acggctacgt cacgctcgag gggttcgccg cccaccggcc
     5461 ggtcgtcacc ctcaccgata gcggcggccc gctcgagttc gtcgagcagg acgccaccgg
     5521 actggtcgtc ccgcccgagc cgacctcgat cgcccaggcc ttcgaccgcc tctacgccga
     5581 ccgggcgctg gccaagcggc tgggcgaggc cggccacgag acgatccggg ccaccgtgcc
     5641 gagctggccc ggcgtcgtgg cccggctcct ggactgaaga catgggcaac cccgtcgtcg
     5701 gcgcggcccg tcggctgttc cggtgggact accagatgga cccgcggatc gcgtggctct
     5761 cgccgatgcc gcccgcgccc accgggatcg ccacctactc gaaggcggtg ctcgaggggc
     5821 tcgaccggat ccgcttcgcg cgcgagcacc accggatcca gccggtctgg ccgatcaagc
     5881 cctcgcacga gggcgcgatc ccctggcaca cgatggccgt gtaccacctg gggaacaacg
     5941 tcgagttcca ccgggacatc taccgccacg cgatccagac cccggggctc gtcgtcctgc
     6001 acgacctggc gctggacgat ttcgtccgcg gcctggtcac gggcggcgat ccgctcggac
     6061 accaggctcg ccgggaggcg ctcgcgaacg ggccgaggct cgaggggttc gaggacgccc
     6121 tgcgcaacga gccgctccgc ctgccgtggg ccgcgcacgt cgcccgccgg gcccggggca
     6181 tcgtggtcca cgccccgttc tgcgagcggt acctgcgggc gttcgggtgc cgcacgccgg
     6241 tctacgtggc gccccacccg gtcctcgagc gcgacgagga cgtccgccgc gccgagcgcc
     6301 ggcgcgcggt cctgcgcgcc ccgctccagg tccaggggat gaggaccctg gtgggggcgt
     6361 tcggggacct gaacgcggcc aagctgctcg acgtgctgtg cgaggccgtc cgccgcctgc
     6421 ccgaggacgt ccacctggtc ctggtcggcc gccggatcga gggctacgac gtcgacgcgg
     6481 tggtcggcgc gagcggactc gggggccggg tgacgctcct gcccgacgtc tcggacgcgg
     6541 acttcctggg ctggctgtgc gcggtggacg tggccgtcga cctccggttc ccgcaccggg
     6601 gcgaggtgag cggctcgctg gcccgcgcga tgcagtgcgg gcggcccacc gtggtctcgg
     6661 ccaccgggac gtacctcgac gtgccggacg aattcgtcct tcgggccccg cccggccggc
     6721 ccgatcctga cgcggtgacc gccgtgctcc ggcggctgat cgaggacgag gggctccgcg
     6781 agcggatggg cgccgcggcc cgggcccaca cggccgagca ggcccgtggc gaccggaccg
     6841 cacacgtcta ctccgcggcc atcgagggca cgctggcgct cctgcgcgac cccgggcggc
     6901 gagcgctggc ccggtgggcg ggggccctgt ccgacctcgg cgtcaccgag gagcacctgg
     6961 gccgcgacta cggcgtgagc tacgcccggg ccctggacga gttcgagcct ggcgcagggc
     7021 ccgtggggtc cgaggcaccg acggcaggag aggggccgag ctgagcacgg ccgcggacga
     7081 ggggagcggg ctcggcgatc gcgtgctggg cgccgggcgc cgtctgggca gccgggccgc
     7141 ctacgaggcc aagaccctcg tggccgcctc cccccggctc acggtggccg cggcccggtg
     7201 gcgggggcac ggggaggtgg tcgggcccgg gtccgacatc ctgatcgagg gctacccgcg
     7261 ctccgccaac cagttctcgg tggcggcctt ccggctggcg cagggccggc cgatgcgggt
     7321 ggcccaccac acgcacgcgc ccggccacgt gctggcggcc ctgcgtctgc acgtccccgc
     7381 gatcgtcctg atccgagacc cgtgcgactc ggtgctcgag ttcgtcctgg tccgtcccac
     7441 cctgaccgtc cggcaggcgc tccgagggtg gatccggttc tactcggccc tgcagccgca
     7501 ccgggcccgg ttcgtgaccg gccccttctc cgaggtgact tccgacttcg gcgcagtgat
     7561 ccggcgggcg aacgagcgct tcggctcggc gttcgtgccc ttcgaccaca ccgaggagaa
     7621 cgtgcgcgag tgcttccggc agatggacgc ctactgggag gggcggatcg gggccaccga
     7681 ggagctcgaa cggtacgtgg gccggccctc ggccacccgc gaccggtgga aggacgacct
     7741 gctcgagacc tacctccgtc tgccgagcgc cctccgctcg ggtgccgagg agctgtaccg
     7801 gaacttcgcc gggggagcgt cgtgaccgcc caacgatcgt ccgccgcgaa cctcggctcc
     7861 ctcgccgcgg agggcaagcg cctgctgcgc cgcggcgcct acgaggcgaa gagcctggtc
     7921 gccaggtacc cggcgctggc ccttcccatc gctcggcgcc ggcacggggt cccggtggac
     7981 gagcgcaccc gcatcgtgat cgaggggttc ccgaggaccg gcacctcgtt cgcggtggcc
     8041 gcgttcgacc tggcccagga cggcggcgtc ccgatcgcct gccacgtcca cgcgccggcc
     8101 cagatcctgg agggcatccg ccgcggcctg ccggtggtgg ccgtggtccg cgagcccgag
     8161 gacacggtct tgtcgttcat gatccgcaac ccgcatctgg aagcgagcac gtcgttgcgt
     8221 ggctacctgc ggttctacga gccgctccgc ccccacctcg gccgcatcgt ggtcggcacg
     8281 ttcccgcagg tgacggcgga catgggcgcg gtcatccggc gggtgaacga gcggttcggc
     8341 accgggttcg cttccttcga acccacggag gacaacgtcc gccggtgctt cgaggcgatc
     8401 gaggccgact accgcaagcg gctccccgag ggcgaggcgc tcgagcgcga ggtcgcccgc
     8461 ccctcggcct ggcgccggga gcggaaggag gagctccgag cggcctatcg ctcgccgtcg
     8521 accgcgcgtc tccgcgcccg cgccgagcgg gtcttccagg cgttcgccga tgccgccggc
     8581 accggcgacc catgacacgc atcctcatcg agacgttgct ggcggcccaa ccggtggatc
     8641 cggaccggca tctggatcat gcgcgcatgc agcggttcac ggcgatcctg gacgagcttc
     8701 cgccggtcgt cgtcttcgac acgccggaag ggatgctgct ggccgatggg taccaccgcc
     8761 tggccgcggc caggcggctg ggacgcgatt ccctcgaggc agagatcaaa cgagggaccc
     8821 gacacgacgc gctccggtgg gccgcccaaa acgccgcggc cgagcggggg atcagcgagg
     8881 gagaagccct tgggcacatc gagcgacacc gcggcggtcg aaggccggac tcgaagggag
     8941 cgggggaggt tgggcgtgga tcgcggtagc tcggcgggac gcctggccgc ccgctccatc
     9001 tacgaagcgg gcacgctcgt cgggcggtgg ccgagcgtgg ccctcccggt cgcccggtgg
     9061 cgcggccacg gcgagccgct cgacacgcac accgagctgg tggtcgaggg cttcccccgg
     9121 tcctcgaact cgttgaccgt ggccatgatc cgggccgccc agcccgaggg catccgcatc
     9181 gcccaccacg tccacgcgcc gagccacgtc ctggagtccg tgcggaggcg gttgcccacg
     9241 ctcgtgatga tccgcgagcc ggaggacgcg gtggtggagc tcgccctgct gaaaccggcg
     9301 atcaccgtgg cccaggcgct ccgcggctgg gtgcggttct acggaccgct ggaaggccat
     9361 cgccgcgagt tcgtggtcgc tcgggccgag gagatcccgc tgcacgtggg ccgggtggtg
     9421 gatcgcctca acgagcggtt cggcaccgac ctcctcccgc cggacgtcgg acccgaggat
     9481 ctggggcggg cggaggcgga taccgagggg tactggacgg gacgctcggg ccccgggctc
     9541 ccgctgatcg gtcgtaccga ggctcgcgat gtgggagctg tcggcggggc cggccaagcc
     9601 ggcgaaggag cggatgccgc cgaccaggcc gagcgccggg ccctgcaccg ggcctacgcc
     9661 gaacgacggc tcacgcgcct ccgatcccgc gccgagcgcc tccacgagac cctcacgggc
     9721 ggcctccccg acctgggctg atccgccggg ggccgacggt agacttggcc ggtacccccg
     9781 atggcaaccc gatccgcatg agaggagcca tggccgccga cgccgtcacg gccgtcaccg
     9841 tccgcacctc cgagcccccg cggctccgga tgatcgatcg catgcgtgcg gtgcattcgc
     9901 accgggagat cctcatgaac ctggtgcgca aggagctgaa ggtcaagtac gcgcactcgc
     9961 tcctgggcgc gatctggtcg ctgctcaacc cgctcgtgtt cctcgctgtg ttcacgttcg
    10021 tatcgaaggt actcggaagc gcgattccgc actatgccgt gtacctgctc tccggcctgc
    10081 tggcatggaa cctgttctcg gtgtccttgc tcaccggcgc ccgatcggtg gtggacaacg
    10141 cgaacctggt gaagaaggtg gccttcccgc gagagatcct gccgttgtcc tcggttggcg
    10201 tggccctggt cgactacgtg ctgcagatgg cggtgctact cctcttcatc gtcgccagcg
    10261 gctatggctt ccacgtgccc gagctggcgc tgttcccgct ggccttcgtc acggtcctgg
    10321 tgttcacggt ggcgatgacg ttctggatgg ccgctctcaa cgtgcggtac cgggacgtgc
    10381 agcacctgct gaacgtgggg ctgctggtgt ggttctgggc cacgccgatc gtctacggga
    10441 gctggcaggt ccaaaacatg ctgggtaacc gggccatctg gggcatgtcc ttattcgacc
    10501 tgtacttcgt gaaccccctg acgccgatca cgctcgcgct gcagcggtcc ttgtacgcgt
    10561 tcgtctcacc cctctcgggc gggcagccgg tgccggtcct ggcccacgtg agcctgccgt
    10621 ggagcgcgat cgtgaccggc ctcgtcctgg tcggctcgat gctgttgctc cgcctcacct
    10681 ggggcacgtt cttccacctg tccggggact tcgcggagga gctgtgaccg cggcgatcga
    10741 ggtcgaccac gtctcgaaga agttccggct gttccgggag cgcccgacct cgatcaagca
    10801 gcggatcctc acgtcgcgct cgcgggccga ggacttctgg gccctccggg acatcacctt
    10861 cgatatcccg gagggctcca cgctcgggct gatcgggcac aacggctcgg gcaagaccac
    10921 cttgctgaag atcgtggccg ggatcctgcg ccccaccgcg ggcacggtcc ggcagcgggg
    10981 ccggctggcc gcgctgctcg aactcggcgc gggcttccat cccgagctca cgggcaagga
    11041 gaacgtctat ctgaacgcct cgttcctggg cctgtcccgg cgggagaccg accgggccta
    11101 cgacggcatc gtcgacttcg ccgagctcga ggggttcatg gacaacatgg tgaagttcta
    11161 ctcctcgggc atgctggtcc ggctgggctt cgcggtggcc gtgcacgtgg acccggaggt
    11221 cctgctgatc gacgaggtct tggcggtggg ggacgaggcc ttccaggcca agtgcctgga
    11281 ccggatcggc cggttccagc gcgagggccg cacgatcgtc ctggtgaccc acgcgcttga
    11341 caccgtgcgc tcgatctgcc accgagccgc gatgctgcac cacggctcgc tccacgcgat
    11401 cggcgcgccc gacgacgtgg tccgcgagat gcgctacgtg ctgctcggtg tgaccgatcc
    11461 ccgcttcgtg cccgaggagg gcacccgcga ggccgagatc gcctcggtcg agatcgtgcg
    11521 ggccgacggc aagagcgagg ggccggtgct gaagggcgat ccgctcacga tccaggtgga
    11581 cgtccgcacg aaccagccgg tggacgacct ggacgtcgac ttccaggtgc tggacggggc
    11641 cacgaaccac gcggtgctgg gcgcccggac ctcccaggac ggctcgccga tcgagcggtt
    11701 cgacggcaag aagcgcgtcc tcttccggat cggcgcgttc ccgtacaccg ccggcaagta
    11761 ctgggtgacg atcggtctgt cctcgcgcga gacggggaag ctgtaccacg tgcagacgca
    11821 gcggtacctg ttcgaggtga tcgaggctcc ccgggtgcag gaggtcctgg acgtgccggt
    11881 ccagagcgtc gtggaagacc tgtaggtgga gggcctgtag atggacccgg tccccgtcac
    11941 acccccccag gcgcccgagc gccgtggggc tcacgaaggg aacacgctca tgctgatcgt
    12001 cctgatcctc gtctcggtga cgctcgccgc cgtggctcag ctcacgctga agcacggcat
    12061 gaaccaggtc acggcgcgga ccggcgtgct ggagctgacc ggcgagtcgg cccgctcggt
    12121 ggtcatcacg cctgcggtct ggatcggcct ggtcctgttc gggctgtccg cggtggtgtg
    12181 gctcggcgtg ctgtcgcgga cgtccctgtc gttcgcgtac ccgttcgcgg cgatcacgta
    12241 cgtgctgatc ctgctgttcg acaagctggt cctggacgag gcggtcccgc cgctgcgctg
    12301 ggccggggtg gcgttcatcg cgatcggcat cttcctggtc tcccggacgc ccagcggctg
    12361 agatggccgg ccccccccat gccgagctcg ccgtggtgat cgtgaactac gacgcgggcg
    12421 agtacctgga gcggtgcctg gcctcgctgg agcggcaccg gggcgacctg gaggtcgacg
    12481 tcctggtgat cgacaacgcc tcgcacgacg gcagccctct cgcggcccgg tccgcgcacc
    12541 cctgggtccg gctgatcgag aacccggtca accggttcct gtcgccggcc tggaaccagg
    12601 gcgccgccga gacccgcgcg cccttcctgc tgttcctgaa cccggacacc gagtggtggc
    12661 aggggacgct cgaggactac gtggaggagg ctcggcggca tccgaacgcc ggcatcgtgg
    12721 ggcccgtggt gcgcaacctc gacgggaccg tgtacccgag cggccgggtg ttcccggctg
    12781 tggtcgacgc cgtggcccac gccttcctgg ggacgctgat gccgcggaac cggttcaccc
    12841 gccgctacca gctcgagggc tgggaccgga gcaccgagcg cgacgtggac tgggtctccg
    12901 gctgctgcat gctcatgccg cgcaaggcct tcgaggccgt cggggggttc gacgagtcgt
    12961 tcctgctcta cggcgaggag ctggacatcg ccacccgctt gcgccgcagc ggatggagcg
    13021 tgctcttcac gccccgggtg gaggtcgtgc acgcgatcgg cgtgtccacc ggccggtcgc
    13081 gggccatgct ggtccgccac tcgcagagca tctaccgcta ctaccgcaag caccgcgccc
    13141 cgggctggcg acggctgacg ctgccgctcg cgtgggtggt gttgcgcctg cgggccgagg
    13201 tggagtggct gcgcgcgggg ccgcggtcgt gaaggcgatc gtgctggtgg ggggcgaggg
    13261 cacccggctc cgtccgctca ccgagacgat ccccaagcct ctgctcccgc tgatggaccg
    13321 gccctccctg gaccacgtcc tggaccacct ggcgcggcac ggcgtgcacg aggtggtgct
    13381 ctcctcctcg tacctcgagt ccaccttcca cgcgttcatc gagtcgcgcc ggggcgaccc
    13441 ctcgatcacc tggatcaccg aggaggcgcc cctcggcacc gggggagccg tgatcaacgc
    13501 cctgggaacc gtggatgacg agccggtctt cgccctcaac ggcgacatcc tgaccgacct
    13561 ggacctcacc gcgatgctcg ccttccaccg gcagcggggg gccgccgcca cgatcgcgct
    13621 ccaccgcgtg gaggacgccc ggcccttcgg cctggtcctc accgagcccg aagggcgggt
    13681 gctcgagttc cgcgagaagc cccaggagct cgtccccggc gagatcaacg ccggcacgta
    13741 cgtgctcgac ccgcgggtga tccgagactg gcccacggga cgggccatct cgatcgagcg
    13801 ggagatcttc cccgagctga tccgtgccgg gcgcccgctg ttcgggttcg cctccgacgc
    13861 ctactggctc gacctgggca ccccggagaa gtacctgcag gcccacttcg acatcctgga
    13921 ggggcgggtg ggattcgagc cgccgttccc ctcgccgtac gtggcggagg acgcggagat
    13981 cgacctgcga tcgcacctgg gccgatgggt cgtgatcggg accggcgcct cggtgggacc
    14041 ggacgcgcag gtggacgaca gcgtgctgca ccccggggcg cgcgtcgagg ccggggccag
    14101 ggtggtcggc tcgatcctgg gcccgagctc ggtggtgggg gccggggcca ccgtgatcga
    14161 ctccgccctg gccgagcggg cgcgcgtccc gcccgggctg ccggctcggg gcctgcggct
    14221 ttcggcggga cgggaggctg ccgctccaga gggggcttga cgggcccacc ggggtggcgt
    14281 gtagggtgcg ggcgcgttcc cggccaacct tcccatcatg atccgacgag ccctgatcgc
    14341 cgctctgagc ctggccctgc tcctggcccc ggccgtcccc gccggcgccg ccgactcctt
    14401 cacgttcttc ggatccggat gggggcacgg cctgggcctg tcgcagtggg gggcctacgg
    14461 tctctcggag cagggctggg ggaccggtcg tatcctcacg cacttctact cggggacgcg
    14521 ggtgaccagc gaggcccagc ctccgcgcct ggtgcgggtg gggctggccc agggcagggc
    14581 ccggttccgc ctgagcgcgc tgggcggtgc ggtcgatctc cggctgggcg atcgcgatcc
    14641 cgccagcacc atcgccacga tccccgacgg tgagacctgg accgtccgcc tggtccaggg
    14701 tagctaccgc ctgatcgacg ccaccggcac cgcggtgggg gacccggtgg ggggcacggc
    14761 catgaacctg tacgcggtct tcgaaccgaa cggcgcggtg ctcaaggtcc cggagaccgg
    14821 tcatacctac gcccacggct ggctcgagtt caacatctac ggctgtgcga ccacgtgcgt
    14881 catgcggctg gtcctggcgg tggctcccca gccgtacctc tacgggatcg gggaggtgcc
    14941 cagcagctgg ccgacggcat ccctgcgggc acaggccatc gcggcccgca cgtacgcgtt
    15001 ccgcaagatc cggtccaccg gtcagcatcg tcccggctgc aactgcgcct tgttcgcgag
    15061 cccgatcgac caggtctacg tgggctacga caaggaagcc ggctatctgg gcgaccggtg
    15121 ggtggccgcg gtggacgcga ccaaggatcg ggtcgtgacg tatcggggca cgctgatcga
    15181 ggcctactac agcgcgtcca gcggtgggtt caccgagaac aacgagaacg tctggggggg
    15241 cacccccgtc ccgtacctgc ggggcgtgtg cgaccccggt gactacacca tcgcgaaccc
    15301 caacgcggta tggaagacgg tcctgaccgc caaacaggtc acggcgcgcc tgggcctggg
    15361 gatcggccgg gtcacggggt tcggcgagac ggtccgcggc gtgtccgggc gcatcgtcag
    15421 cgtgatcgtc aagggcacca cggggcgtgc ctcggtggct ggaggcacgc tgcgcgccgc
    15481 gctcggcctg cccgacgatc gggtctggat caaccacaac cgccaggtga ccggcacgat
    15541 ccggcagaag tacgacgcca tcgactgccg gtccggcctg cccacctcca gacgggtccg
    15601 agtggccggt ggggcgcgcc aggtcttcca gctcggcacg atcttcgcca aggacggcat
    15661 cggcgcccac gaggtccggg gcacggtcct ggccttctac cgcaagcacg gaggtccccg
    15721 cgggtccctg ggcttcccca tcaccgacac ccaccggctg aagaacggga acacgcgctc
    15781 gcggttcgag cacggcaccg tgacctgccg accgtcgggc tcctgctggc gctcgtagct
    15841 cgagcgcagg gccgcgccgc tacgggttca gggtggacca gtcgtaggtc gaggggatcg
    15901 gcgtgggttc ctgggcgctg gtcccgaagg tgaggaccac gacgtcgtgg tcggccaacg
    15961 caacgtcgcg agggctgccg gtgatcggcg agccgttcac gtaggccctg attcgctcgg
    16021 cacccgagtc gcagtacccg ccgagacagg tccgggtcag ccgcaccccc cacacgtcga
    16081 acacgttgcc caccgtgaac gtgcgctggg tggcggactc gacgtggaac acgccgccgc
    16141 tctcgtgcgt gtgcagcggc gattccacgc cggaggccag accgatgtcg gccgggaccg
    16201 gctccgcgga acccttcacg aagatctgca ggtccacgtg gatgtgctcg gcgccgccgg
    16261 cggccggcag gtcgagccgg tccagccggg ccccgacctg cgccgtgttg gccggccacg
    16321 gggccgtgtc cttcaacaac ccggggaggg gaccgctcgg cgtcgagcgg gggcccatca
    16381 ccaggatgat gatcgacacg gagacggcgc tgatccccac caggatcagg taggtgcgga
    16441 cgaaccgtcg acgcttggcg cgcttgcgct cctgctcccg gcgctgccgc gcgagctcct
    16501 tgtgctccgc gcgtcgggat cgagccccct cctccaccgc ggtggcggtg cccccgcccg
    16561 ggggacggga tccgcgcgac ctgcggcgct tcttggccat gtcggctagc cttggaagct
    16621 cgaccagtcg aacgtgggca ggggctgggg cacctgcgcg gcggtcccga acgcgaccac
    16681 gacatcgtcg tgctccttca gcgggaccga ccgggggttg ctctgcaccg gcctcccgtt
    16741 caggaacacc cggagctgct ggtcaccctc ggtgcagtac ccgcccaggc aggagctggt
    16801 gaggcggacg ccccacacgt cgaagaactc gccgagcgtg gggctgaagc tgggattgcc
    16861 gctctcgatg tggatcacgc cggtggtgtc gtgggtgtgc aggggcgagg cggtggagac
    16921 cgcgagcccg atgtcggccg gcaccgtgac cggcgagccg tccaccgaga tcgccaggaa
    16981 ggcgtggtgg tgcagggccc caccctccgg cggcagcccc aacacgcgca gccgctggcc
    17041 caggtcctgc gtgttcgccg gccagggggc cttggagtcg agcacgccgg ccagctggcc
    17101 gggcgtggcg gtgctcgagg gcgatctggt ggagacgacg gccaccacgg ccgcccccac
    17161 gatcaccacc gcggcgatca ccgcgagccg tcggttccgg cgggtccgcg ccatcttgcg
    17221 ctggagctcg agccgttccc gccgggcctg ctcccgacgc tcggccttcg tcatgcgccc
    17281 ctggttggtg cctcggttcg tcccctgctt ggccacgatg ctccttccct ccgcgggtgg
    17341 ccgcgagggt cccaggatat cgggtggggc cctcgttgct ccggaccacc gagctcccta
    17401 tgcttcggtc ggtcttcccc gtggtgcccc gagggcccgc accggggccg tgtcgagcgg
    17461 tcgaaggggg cgtgcatggt agggacgcgg gaacgacgag cggccgggct cgacggcttg
    17521 ttccacatca ccgagcgagg atccacggtc aggacggaga tcgtggccgg gctggcgacc
    17581 tggctgacga tggcctacat cctgttcgtc aacccggcca tcctcggatc ggtccaggac
    17641 cacaccggca cctcgctgcc gttcggacag gtcctcacgg tgacggccct cacggccggg
    17701 gtgatgacca tcctgatggg ggtggttggg aactacccgt tcgcgatggc cgcggggttg
    17761 ggcctgaacg cgttcgtggc gttcacgctc gtgagcgggt acggcctgtc ctggcccgac
    17821 gcgatgggcg tgatcgtgat cgagggactg gtgatcgccg ccctggtcct cacggggttc
    17881 cgggaggcgg tgctgaacgc catcccgatg gacctgaagc gggcgatcgg gatcgggatc
    17941 ggtctgttca tcgcgttcat cgggctggtg aacgccggtg tggtgatcca tccgcggggt
    18001 gggacgatcg tgaccctcaa cccggatctc accacgctga ggatgctgac gttcgtgatc
    18061 ggcttggggc tgacggcggg gctggtggcg cggaagatgc gcggggcgct gctgatcggg
    18121 atcgtggcca ccacggtggt ggccatcgcg atcaacgaga tctgggggca ctcgaggatc
    18181 tggcagacgg tgggtcccgg cgtcgcgcag atcccgtcga ggatcgtgga gtcgccggac
    18241 ttcagcctcc tgggcaactt ctcgatcgcc ggggtgttcg acgcgctgcc gttcttcacc
    18301 gcgatcgccg tgatcctctc ggtgatgctg tcggacttct tcgacacgat ggggaccgtg
    18361 atcggcatcg gcggggaggg caagctcctg gatcgagaag gcaggctgcc cgggatcggg
    18421 aaggtcctgt tcatcgactc gctgggggct gccgccggag gagccgcctc ggcctcctcg
    18481 aacaccacct acatcgagag cgcggccggc gtgtccgagg gaggtcggac cggcctcacc
    18541 tcggtggtgg tcgggatcct gttcctgctg gcggtcttcc tgtcgccgct cgccgaggtc
    18601 atcccgcccg aggcgaccgc gccggtcctg gtgatcgtcg gctacttcat gatgacgatc
    18661 gtccgcgaca tcgagtggag cgacccgacc ctggggatcc ccgcgctcct gacgataatc
    18721 gtcatgccgt tcacgtacag catcacgaac ggcgtgggcg ccggcttcgt gtcgtatacg
    18781 gtgatcgagc tgctgcgggg ccggtggcgg aaggtgcacc cgctcctgta cgtcgtgtcc
    18841 gcgatcttcg tctggtactt cgtgcgaggg ctcctggcct agggtctggc tgcacgcggt
    18901 cgagggcccc ggcgggaact ccggggcctt cgctcccccc ggcctccgct ccccgtcggg
    18961 ggcccggggc cgccggggct accggaagtc ggcgaggtgc ccgaggtcga tttccgcgcg
    19021 cggggccggc cgcccgtccg gcatcggtcc caccgccacc gtgccgagcg cgcgccactc
    19081 gtcgcccatg ccgaggaccg cccgggtctc ctcctggcag aacagggtcg aggagatcca
    19141 gcacgaggcc agtccctgcg catgcagggc cagcaggagg ttctggatcg ccgcccctcc
    19201 cgacagcagg aacatctccc gctcggcggt tgcgcgctcc cggtcggggt acgggtgcgc
    19261 gccgcggaac cgcacccacg ggacgatcaa gaccggcgcc ccgcccagca ccgcgtcgct
    19321 gcgggcgatc cgccgcgcga tcgtgtcctc gggcgtgccg tcaccccgca ggtcggcgcg
    19381 ccaggcctcg gcgatcgcgc cgagcagggc gcgccgggac ggggccgacg ccaacaccgt
    19441 gaagctccag ggtcgggtgt ggtgaggagc cggcgcggtg cacgccgccc gcaccgcctc
    19501 ctccagcgcg ggtcgggcca cgtccccggg tccgaaggaa cggatggtcc gcctcgccga
    19561 gatcgacacc agcggcgact cccggaacag gtcttcctcc ggcgggcgga tcaggtccgc
    19621 ggcctcgccg tcctcggcct cggcgtggat cccccggacc accgcgaccg gcacccggtc
    19681 ggccttgccc atcaccagcc cgctggccgc ggccacctcg tccgccaggg ccaccaccgt
    19741 ggcctccagg atccgtcccg tgtgatcggg ccgcccccgg aggtcgacca gcgccgggag
    19801 cccggcgcac ccgatcgcca cgttcagcag cccccgccgc caggcccgtc cgaaggtgtc
    19861 ggtgatgacc acccccaccg gagccccgaa cgccgcggag atcccctcgc ggagccgccg
    19921 ggcggaccgg tctggatcct cgggcagcag ggtgagcagc cccggctcca cgttcgaggc
    19981 atcgaccccc gcgttggcgc agacgaagcc gtgccgcgtc tcggcgacca ccagctcctc
    20041 ccggcgggcc accacgcgcc gcgtctcgcg ctccacccag gccgcccgcc caccgccctc
    20101 ctcggggacc acgcgtccct cggccttgga cacgatcttc tgcgtgaccg cgatcacgtc
    20161 gccgtcgcgg aggcccaccg cgcgcagggc cggcgtgagg acgacgatca ggtcgtctcc
    20221 cggccggacc tcggggatcc ccgggacggg caggacctcc acgctcaccg ggccctcacc
    20281 gcagcagatc cagagcggtg cgggccagct cggtcgtgac gcggtcgtcg cgcatgatcg
    20341 tgtcggtcac ggccacgcgg atcccgagtc gctcctcgat cttcggggcc agatcccgat
    20401 cggcgccgtc gaccacccag ccggtcaaca gcccttcgta ggccttcgcc gccccaaggg
    20461 cgctgacctc caggccgacc accggcatca gcttgtccgc catgccggcc aggacggttc
    20521 cgcccacgat cggcgagatg cccaccacac gatcggagcg ctcccacagc gcgtcccgga
    20581 tgcccggcac cgccaggatg gggccgatcg agaccaccgg gttcgatggg cagatcacca
    20641 cggcgtcggc ggtgcggatc gcctccagga cccccggagc gggccgagca cggatcgctc
    20701 cctcgaaccg gaccgctcgc accggcggct cggccccacg gcggacccag tactcctgga
    20761 agtggacgtc gtgccgcagc gcgtcgggct cctggatctg caccatcgtg gtcaccggat
    20821 cgtcgctcat gggcaggacc gtccacggga gctcgaaggc ctcgaccacc gccgcggccg
    20881 ccgccgtgag ggtccacccc tcgcggagca gctcggtccg gtacaggtgg gtggccaggt
    20941 cacggtcccc gagggcgaac caggtgggaa gcatgttctg agcctcgagc tcgtcgcgga
    21001 cccggaaggt ttcgtcgccc cgaccccagc cgcgctcccg gtccatccct ccggccagcc
    21061 agtacacgac cgaatccagg tccgggctca cgtgcagtcc ccacagctcg atgtcgtcgg
    21121 cggtgttcac gatcacggtg aggccgtgcc cctcgtcgaa ggccagccgc tgcagcccac
    21181 gcaggagctt ccccgccccc acgcccccag ccagcgccac cagttccacg gccgatagcg
    21241 tagtaggctc ccccggtatg gcggacgcca tctcccacca gacccgacgg gccctggcgc
    21301 gcgccgaagc cggcaaggcg ctgtcggtcc cggaggcgga ggcgttgctg tccgcccgcg
    21361 gacctgagct gcggcgcctg atggaggtgg cggcccgcct tcgagacctg ggccacggct
    21421 cggtcgtcac ctactcgccc aaggtgttca tcccgctcac gatgctgtgc cgcgaccact
    21481 gccactactg caccttcgcc aagccgccgg cgcatctcga gcagccgttc ctgtcccccg
    21541 aggaggtggt ggcgatcgcc cgggccgggc gggacctcgg ctgcaaggag gccctgttca
    21601 ccctgggcga caagcccgag gaccgctacc ccgtcgcgcg ccggtggctt gaggagcgcg
    21661 gctacggcac cacgctcgag tacgtccgag ccgtggccat caaggtgatc gaggagtccg
    21721 gcctgctgcc gcacctgaac ccgggcgtga tgagctggga ggacatggcc cgcctgaagc
    21781 acgtgagcgc ctcgatgggc ctgatgctcg agaccgcctc cgagcgcctg tcggggcggg
    21841 ggatggcgca cttcaactcg ccggacaagg tgccggccgt gcggttgcgg acgatcgagg
    21901 acgccggacg cctggcggtc cccttcacca ccgggatcct ggtggggatc ggggagacgg
    21961 cccgcgagcg ggccgagtcg ctgttcgcga tccgggagct ccatcgacgt taccggcacg
    22021 tccaggaggt gatcgtccag aacttccggg ccaagccccg cacggggatg cacgcgtcac
    22081 ccgagccctc ggaggaggag ttcctggccg ccgtggccac cgcccgggtg gtcctcggtc
    22141 cggccatgag cgtgcaggcc ccgccgaacc tgtccgaccc ggaccagcag ctgcgcctgt
    22201 tggatgccgg catcaacgac tgggggggtg tctcgccgct cacgcccgac cacgtgaacc
    22261 ccgagcgccc ctggccccag atcgagtccc tggccgcgcg caccgccgag cgcggcttcg
    22321 aactcaggga gcgcctcacg atctatccgg gcttcgcgct gcggcccgat ccctacctgg
    22381 ccggcaagat gcgggcgccg gtggtcgcgc tgatgggacc ggatggcctg gcagcccccc
    22441 atcgccagcc cgagcccatc gcctggcagg atcccgacgt cgcttggaag ccgaggagca
    22501 ccgcccccac cttcgccaag gctccggagg ccgggttgcg cgaggacgcc gcggcggtct
    22561 acggggacct cgaagcgctc gagaacgagg tgacccggag gtgggcccgg cgggaggtct
    22621 tccccgagcg gatcgacgcc ctgaccaaga gggcgctgtc cagggcggag gcccaccggc
    22681 cgatcacgga cgacgaggcg ctggcgctgt tccaggccga gggcccggcc ctggaggccc
    22741 tgtgccgggt ggccgacgat cttcgcggcc aggcggtggg cgatgaggtc acctacgtgg
    22801 tgaaccgcaa catcaacttc acgaacgtct gctatgtggg gtgccggttc tgcgccttcg
    22861 cccagcgcga ggccgacgcc gagtcctaca ccctgaccct gcaggaggtg gccgaccggg
    22921 cgcgggaggc cgcggagtgg ggagccaccg aggtctgcat gcagggcggg atccacccgg
    22981 acctgcccgg cacgttctac ttcgacctgc tggacgcggt gaaggccgca gcgcccgaca
    23041 tccacatcca cgcgttcagc ccgatggaga tcctgaacgg cagctcgaag ctggggatca
    23101 ccttccggga gttcctgacc gagtgccgct cgcacggcct ggggacgatc cccgggaccg
    23161 cggccgagat cctcgacgac gacgtccggt gggtcctgac gaagggcaag ctgcccgccg
    23221 acacgtggga gcagatcgtc cgaaccgctc acgagctggg gatccgatcc tcgtccacca
    23281 tcatgtacgg gcacgtggat gccccgcccc actgggtgtt tcatctgcgc cggctgggag
    23341 cgatccaacg ggacaccggc ggcttcaccg agttcgtccc gctcacattc gtccaccaga
    23401 acgccccgat ctacctggcc ggcaaggccc ggcccggcgc caccttcgcg gagaaccgcc
    23461 ggatgcacgc ggtggcccgg atcctgctgg acggcgccat cgacaacatc caggtctcct
    23521 gggtgaagat ggggaccgag gcctgtcagc tgatcctgca gggcggcgcc aacgacttcg
    23581 gcggcacgtt gatggaggag acgatcagcc ggatggccgg cgcgcagtgg gggatccgca
    23641 tggagccgtc cgagttcgac gcggcgatcc gggccatcgg gcggatcccc gccgaacgga
    23701 ccaccacgta cgaacgcttg cgccgcaacc actccgccga catccccgtc gccggatagc
    23761 ccgcatcgtc aggcgtcccc atgggccgca cgcgcgtgct catgctctcg gcgcgtccga
    23821 acgaccgaaa tggtcaagga gattgacgag cggttttctg tcgtcaccct attgaaaccg
    23881 cggacgcggc acaccacgat tcgcgatggg gagatgcacg tggagatgtt ggggacccga
    23941 agatcccctc ttgtcaaagg ggggaggagt ccctcagaat cacaggcgtg tgattcgagg
    24001 gggagagtgc cgtgcatcac tacatcccgt ggcaggaacg agcccggtgc cgggagcatg
    24061 accccgagat cttcttcccc gagaagggag ggtcgtcgag agaggcgaag cggatctgtg
    24121 ccgactgccc ggtccgcatc gagtgcctca actacgcgct gcgccgcgat gagcggtacg
    24181 gggtgtgggg tggcatgagc gaacgggaac gacgacgcct gaagcgtatg gcgtcctgac
    24241 ggggaaaggg ggtgacccat gtctccagcg aagaagaggt cgaccgcgaa gaagaagacc
    24301 accgcgaaga agaagaccgc ggcacgcaag cctgcagcca agaagaccgc agcgcgcaaa
    24361 cggacgaccg cgaagaagcg gtagatcccc agggcgctgt ggaaccgatc gtgagggggg
    24421 cgcgggctcg cgcccccctc tccggttccc aggtccgcgc gtgctaggct gcccgccgcg
    24481 caagcgcacc catggccacc acaaccgctg atcgcgccgc ctcgaacggt gccgtccgca
    24541 tgccgagcgt cctcgcggtg ctcgtcgtga aggacgggct gccgtggctg cgggagtgtc
    24601 tgcagtcgct ctcgggtcag tcgtacgcgc gcttcggggt cctggccgtc gacaacggat
    24661 cccaggacgg gtccgtcgac gtgctggccc gggccctggg gccgggccgg gtcctacggc
    24721 tgagcgagga ccggggggtc gccggcgcgc tccgagcggc cctggagctt cccgccgccc
    24781 aggccgccga ctacctcctt ttggtgcacg acgacacggc gctggccgag gacgccgtga
    24841 ccaggatggt ggaggccgcc gagggcatcc ggggtgtgga gcgcgtgggc gtggtcggcc
    24901 cgaaggtggt cgactgggat catccgcgcg tgctccgcga ggtcggccgt tccaccgatc
    24961 ggttcgggca tccggatacg cccctgcagg agggggagct ggaccacggg cagtacgacc
    25021 gggtcctgga ggttctgtac gtcccaacct gcgccatgct gatctctcgt gctgcctggc
    25081 agcgcaccgg gctcctggac gagcggctgg ccgggcacca cgacgacctc gacttctgct
    25141 ggcgggcgcg ggtcgccggg ttccgtgtcc tcatgacgcc gctggcccga gcccggcacg
    25201 tggcggcggg gacccacggc gagcgcccag gcgctccggc ccgccgacat agcgatggct
    25261 accaggcgga gcgcgcggcg ctggcctcca tgctgaagaa ctacggggtg ctgtccctgg
    25321 tctggctgct gcccctgcac gccctcttcg gcctgatccg cctggtctac ctgtcgctgt
    25381 cccgacggtt cgaggaggtc ttcgacctgc tgtcggcctg gatgtggaac gtcctgcatc
    25441 tgccctccac gctgcggcgg cgcgtgcggg cccagtcggt gcgctccacg ggggaccgag
    25501 cggtccggcg cttcatgcag tcggcatggt tccgcgtgcc ccgttggttc cagaccgcgg
    25561 agctcctcct cgaggaacag gcccaggagg aacgtgagca ggtccggatc cgggcacggg
    25621 cggcgtccct ggccacggag caccccgtgc tggtcacctg ggtgctggcg atcgtggtgg
    25681 gcgcgctggc cttccgagag ctgatcggac cgggggccct ccagggtggg gtcctggccg
    25741 ggttcccggg gcacccggcc accttcttcc gggagctggt ctccgccgtg cggaccaccc
    25801 agctcggagg cacccaggcc gcgagcccgg cgctggcccc cctgggggcc ctgtccgcgg
    25861 tgctgttcgc gagccccgcc ctggcgcaga aggtgctctt ggtgggtctg cccgtgctgg
    25921 ccgggctgat gatgtaccgc tccgtccacc gccagaccca gcacgcggtc gcggcctgcg
    25981 tggcggccgg cgcctacacg ctctcggcca tgaccttctg ggcgttctcc gagggccgga
    26041 tcgacgtgct ggtcgcgctc gccgcgttcc ccgccatcgc cgaccgactg gaggcggcct
    26101 tcgcatcgga tggccctgcc gtccggtggc gggcagcggt ggggctgggc atgaccgtag
    26161 cggtggctgt ggcgttcctc ccggggatcg tcgtagcgct cgtgcccctg ttcgccgtcc
    26221 atgtgatcgg gggacgccgg cggatccggg ggaccgggct ggccctgctg ggcgcagcgg
    26281 ttgctgccgt cctggtcttc cccacgctgc ccggttgggc gggcgacccc gg
//
LOCUS       DASQQQ010000253         4517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_293313, whole genome shotgun sequence.
ACCESSION   DASQQQ010000253 DASQQQ010000000
VERSION     DASQQQ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293313"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..336)
                     /locus_tag="VE646_09675"
     tRNA            complement(246..336)
                     /locus_tag="VE646_09675"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(300..302),aa:Sec,seq:tca)
     gene            complement(398..1363)
                     /locus_tag="VE646_09680"
     CDS             complement(398..1363)
                     /locus_tag="VE646_09680"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HZD80293.1"
                     /translation="MGMTLPGFGRLLRALGAVWAVNLDGGASTTMVARGSVVNTPSDP
                     TGERRVLTTVILHSSDALVGAFPLIRQLQRLDARIVLPGREPPPPSGDPPWHPPGHVT
                     TPSHDMDGSAMPHGGWRSRSVAAAVAGAVADTAPPGMVALVGRGRSPSGDRLSRTTRS
                     AEVPADRGAHEPTRLLPSTGHIRIPSIGVDAPLISLGTAPGGTLEVPVDPGVAGWFSD
                     GPFPGEVGPAVVVGHVDWTSGPAVFYRLRDLRRGALIVVLRQGERRSRFQVRSLRWFA
                     KSDFPTQRVYGPVTVPVLRLITCGGAFGGTGHCVDDLVVFAVPLG"
     gene            complement(1528..1953)
                     /locus_tag="VE646_09685"
     CDS             complement(1528..1953)
                     /locus_tag="VE646_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80294.1"
                     /translation="MGARLGTLAVGAATAAALTLAAPTGTGLAMTASGTGRNRFPSAS
                     IDRPSVGRQPSTNADPGHPDRPSSGNLMRVVDSNVVVPGVRFTRIRLTDPPEDVGVLR
                     AGPRARVRALLVRSGTDRAPYATVSSAVDRSHAVAGTNG"
     gene            2757..3302
                     /locus_tag="VE646_09690"
     CDS             2757..3302
                     /locus_tag="VE646_09690"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZD80295.1"
                     /translation="MPSKVEIVILGLLAEGPRYGYELLERYRYRAMEEWVEVGKASVY
                     QALRRLEDSGALTGRQQEGTEGPDRRVYRLARPGRQRLHRTVLESLAEAGPYGSDGGP
                     GLGFVHLLDREEADTVLTAREAALRARRGRLAQARRALVPGADPGDRVAARLLRLQDE
                     LAGTELRWLVALRRDLAELGS"
     gene            3357..4157
                     /locus_tag="VE646_09695"
     CDS             3357..4157
                     /locus_tag="VE646_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005515815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HZD80296.1"
                     /translation="MEAAFFDLDKTIISKSSSLALSRPMYRAGLVSRSQLLRGAYAQL
                     VYLLVGADERKMERLKEGMLALTKGWERSQVEQLVQDVIIDVIDPFVYQEALDLMVLH
                     RQQRRRVYIVSSSPEEVVRPLARHFGVNGVIATRARIVDGCYTGDLEFYCAGEGKANA
                     ITALADRVGIDLEGSYAYSDSVSDLPMLGAVGHPVAVNPDRDLRRVAEERGWDIREFR
                     RPVRLRTRIATAATSTRNQVAAGILAGAGVAVVFGWVVLRSRLAGHRA"
BASE COUNT          702 a         1563 c         1614 g          638 t
ORIGIN      
        1 gggagatcgt ggccctggag accgaacgga gccgtgtcga ggccgagtac gaccttgcac
       61 cctcgctgcc gaccgacgat ctgctcgggg agctccgggc cgctctcctc cgggcctccg
      121 aggagaaggc cgtggagaag ctcaagcagg tgctcgcctg cgtcgtggac acgatcgtcg
      181 tggagggccg ggaccacatc cagccctact acttcgtgcc cggggttctt acagtgtccc
      241 cttcgcggag gcggacggga atcgaacccg ccagggagct ctcgccccct caccggtttt
      301 gaagaccggg ggcgacacca gccgccgttc gcctccgcaa acagactacc cagggcgccg
      361 atggaagcgg gtggctgtgg ggacttcgcg cgccacctca ccccaggggg acggcgaaca
      421 cgaccaggtc gtccacacag tgtccggtcc ccccgaacgc tccgccacat gtgatgagcc
      481 gcaggaccgg gacggttacc ggcccgtaca cgcgctgggt ggggaagtcg gacttcgcga
      541 accaccgcaa cgagcggacc tggaagcgcg atcgacgctc cccctgcctc aagaccacga
      601 tcagggcgcc gcgacgcaga tcccgcagtc ggtagaagac ggccggccca ctcgtccagt
      661 cgacgtgtcc caccacgacc gcgggaccga cctctccggg gaaggggccg tccgagaacc
      721 acccggcgac cccaggatcg acgggcacct ccagcgttcc gccgggggcg gtccccagcg
      781 agatcaaggg cgcgtccacc ccgatcgacg ggatacggat gtgcccggtg gagggaagaa
      841 gccgcgtcgg ctcatgagcc cctcggtccg cagggacctc ggccgaccgg gtggtgcggg
      901 agaggcgatc acccgaaggg gaccgacccc gaccgaccag cgcgaccatc cccggcggag
      961 cggtgtcggc caccgcaccg gcgacagcgg cagcgacgga gcgcgaacgc cacccaccgt
     1021 gtggcatggc cgagccgtcc atgtcgtggg atggcgtggt tacatgaccg ggcggatgcc
     1081 atgggggatc cccactgggg ggcggtggtt cgcggccggg gagcacgatg cgcgcatcca
     1141 acctctgaag ctgcctgatc agcgggaacg ctccaacgag ggcatccgag ctgtggagga
     1201 tcaccgtggt caagacccgg cgttccccgg tgggatcgga tggggtgttg accaccgatc
     1261 ccctcgccac catggtggtc gaggccccgc cgtccaggtt cacggcccag accgctccga
     1321 gcgctcgcaa cagccgaccg aatcccggaa gggtcatccc catcgaggtg gtacgcctgc
     1381 catccacggt gacgaggagg accgatccgt ggcgggtgag tccgaacccg gtcctgggat
     1441 ggcgccctcg gggcagggac cctgggtgga ggccacgaca cgaccggcca gcacgagcat
     1501 gagggactgt ccgggcggtc gctggaatta gccattcgtg ccagccacgg catggctccg
     1561 atccaccgcg gacgacaccg tcgcgtacgg ggcacggtcg gtaccggatc ggaccaggag
     1621 ggcccgcacc cgggcccgag gtcctgcccg aagcaccccc acatcctcag gtgggtcggt
     1681 caaacggatc cgcgtgaacc ggacccccgg cacgaccacg ttcgagtcca ccactcgcat
     1741 caggttgccc gaggaaggcc ggtcgggatg gcccggatcc gcgttcgtcg acggctgccg
     1801 accaacgctc ggtcgatcga tggatgcgct cgggaagcgg ttccgtccgg tgcccgaggc
     1861 cgtcatggct aggccggtcc ccgtgggcgc ggccagcgtc agcgccgcgg ccgtggccgc
     1921 acccaccgcc agcgtgccca gcctggcacc catcctcctg cgacccgtgg cttcagcgct
     1981 tggcgaacgc gaccgcggcc accatcaggc cggccaccga gagcagcgcg agcgtggcca
     2041 gctcccctcg agccctcccc gcggtgcccc acgcccggtt gcgacccccc gctcggcgcg
     2101 cagaaggtgc cgtgcaggga cgcggtcgat tcgggaccgg gctcggtgcc gacggaggcg
     2161 gacgcgtggt cgttcgtggg gttcgggtcg tcggtgctgg atgagacacg gacgtccagg
     2221 tggttgccgt cccccgccga agtcctggag cacggccgtc gcagtgaccg aaccatcgct
     2281 ggcgatggtg cccagggagt atcggatcgc ccctcccgtg tggcggcacg tcccctggct
     2341 ggtgctggcc gagacaccgc ccgcgtccga gccgtacacc gccgccaggc ggacatcgtg
     2401 cgcggccgcg ggtcccaggt tcctgaccct gatggtccag gtgatggtcg aacccaggac
     2461 cactgggtcg ggctcacctg agaggcggac cgtcagatcc tgcttgcagc ggcagccggc
     2521 agtggcatga aggcgctgag cgcgatcgca gcgacagcga tcacgatctt cgaggcgcgc
     2581 atggcggact cctctcaacc gggggtggca gtgccatcgt cgagggatgc gggagcgaga
     2641 ggtaccgtca cgtgcccctc gtccggtggg accctcaccc ggccccgtcc gagcgagacg
     2701 gtccttgctc cggccggtcg cgcctcctag tctgtaccgg actagccggg aggcgcatgc
     2761 cgtcgaaggt ggagatcgtg atcctggggc tcctggcgga gggccctcgc tacggctacg
     2821 agctgctgga acggtaccgg taccgcgcca tggaggaatg ggtggaggtc ggcaaggcca
     2881 gcgtctatca ggccctccga cggctggagg actcgggcgc cctcaccggg cggcagcagg
     2941 aggggaccga gggacccgac cgccgcgtgt accgtctggc ccggcccggg cggcagcggc
     3001 tgcaccggac cgtgttggag agcttggcgg aggccggacc ctacgggtcg gacgggggtc
     3061 cgggcctcgg cttcgtccac ctgctggatc gggaagaggc cgataccgtc ctgacggcga
     3121 gggaggcggc gctccgggct cggcgaggcc gcttggccca ggcacgccgg gcgctggtcc
     3181 ccggggccga cccgggcgac cgggttgcgg cccgcctgct gaggctccag gacgagctgg
     3241 ccgggaccga gctgaggtgg ctggtggcgc tgcgtcggga cctcgcagag cttgggtcgt
     3301 gagcgatccg ggccgataac cagcgcgtcg gtaccgtctc ctaccatggg gggaccatgg
     3361 aggccgcgtt cttcgacctc gacaagacga tcatctcgaa gtcaagctcc ctcgcgctgt
     3421 ctcggccgat gtaccgagcc ggcctggtct ccagatccca gctgctccgg ggagcctacg
     3481 cgcagctcgt ctacctgctg gtgggggccg acgagcggaa gatggagcgg ctgaaggagg
     3541 gcatgctcgc cctcaccaag gggtgggagc gctcgcaggt cgagcaactg gtgcaggacg
     3601 tgatcatcga cgtgatcgac ccgttcgtgt accaggaggc gctcgacctc atggtcctgc
     3661 atcgtcagca gcgacggcgc gtctacatcg tctcctcctc gccggaggag gtcgtccggc
     3721 ctttggccag gcacttcggc gtgaacggcg tcatcgctac ccgggccagg atcgtggacg
     3781 gctgttacac gggcgatctc gagttctact gcgccggcga ggggaaggcg aacgcgatca
     3841 cggctctggc cgaccgggtg ggcatcgacc tggaaggctc ctacgcctac tcggactccg
     3901 tctctgacct gcccatgctg ggggccgtgg gccatccggt cgccgtgaac cccgaccgcg
     3961 acctgcggag ggtggccgag gaaaggggtt gggacatccg cgagttccga cgtcccgtgc
     4021 ggcttcgtac ccgcatcgcc acggccgcca ccagcacccg caaccaggtc gcggccggca
     4081 tcctcgcggg cgccggcgtg gccgtcgtgt tcggctgggt ggtcctgcgc tccaggctcg
     4141 ccggccacag ggcctgacgg ccgcccagca cctcgacggc cggattcgcg ggcccacgtg
     4201 gaccggtgta cgatcggtgt gtcaccttcg ggtgaccgtg aagggagcag aaacccacgc
     4261 gcgtcttgcc gcgggaggca tgctcgagga tccggaggcc gaggcccccg gtcaccgagc
     4321 aagtgcagac agcgcatgtt cgctgctccc ttcgcgctcg tccggggcgc tggcccgacc
     4381 ccgcatcatc cggttcgcag ggccccgttg ccctctcctc gcggcttgaa ccctgaacca
     4441 cgtgggtagg ttgcggcgca gtacggaatg gaatcgacga ggagaaccgc gtggcccaac
     4501 ccttccaaga cgatccg
//
LOCUS       DASQQQ010000254         2577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_15526, whole genome shotgun sequence.
ACCESSION   DASQQQ010000254 DASQQQ010000000
VERSION     DASQQQ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15526"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VE646_09700"
     CDS             complement(<1..462)
                     /locus_tag="VE646_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004003284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZD80297.1"
                     /translation="MPRTNETVEAVLLEYADLLSILSEDPFKPRAYEKAARAVGGYHA
                     DLVELDLKGILAIPNVGRSIGEKIHEYLETGSVAALEELRAQIPAGVRQLMSIPGLGP
                     KRAMVLYEELGITAVDQLLDAIHEERLAGIKGFGSKTQENILQGIQQLHASS"
     gene            complement(484..1404)
                     /gene="argF"
                     /locus_tag="VE646_09705"
     CDS             complement(484..1404)
                     /gene="argF"
                     /locus_tag="VE646_09705"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZD80298.1"
                     /translation="MERDFLSIDDLTPGELDRILDLAAKVKATPEDYADRLHRKAIAL
                     IFEKPSTRTRVSFEVAIAGTGGHPVALSTAELQLGRGETIEDTGRVLSRYVDAIVLRT
                     FEQERLEQLAAAASVPVVNALSDFEHPCQALADLLTVREARGGPGGRVLVYLGDGNNV
                     THSLLLAGAKVAMHVRVATPPGFEPIPQVVHRAREIAQETGGSVEVLNDAAAACRGAD
                     VLYTDVWASMGQEDEAEERALVFPAYQLNQALVELAKDDVTILHCLPAHRGQEITDEV
                     IDGPRSAVWEQAENRLHTQKAVLLWLLGLE"
     gene            complement(1480..>2577)
                     /locus_tag="VE646_09710"
     CDS             complement(1480..>2577)
                     /locus_tag="VE646_09710"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZD80299.1"
                     /translation="EVFDRLRMPVRVVDEDRVEVEVPGYRVDVEREVDLIEEIVRVQG
                     YERVGSSLPAARQPGGLPAGYAFIGRVRRSLVRAGLREVRQVPFVSDTDLALFGDPGA
                     VPVTNPLQADEGYLRPRMAPGLLKALRRNLNRHVRGAALFEIGAVFRSGGKDVREERA
                     AGFVLAGRASQGWAEPERSFDVFDGKGVVEAVLADLGVRGWALGDPPGDPFHPGRSAE
                     VRIGPQVVGAFGELHPRAAASLDLAGRVTVGELDLEALMGRAGSELAVLDLPRFPPVR
                     RDLAFMVAEDTPAGGVQAALEEAAGDLLGGCVLFDVHQGPPLPEGRKSLAFSVDLRAQ
                     DRTLTAAEADAAVAQIVERLGRDLGAELRAG"
BASE COUNT          370 a          999 c          844 g          364 t
ORIGIN      
        1 gctcgacgcg tgcagctgct ggatcccctg caggatgttc tcctgcgtct tggatccgaa
       61 gcccttgatc cccgccaggc gttcctcgtg gatcgcgtcg agcagctgat cgacggcggt
      121 gatgcccagt tcctcgtaca ggaccatggc ccgcttgggc ccgagccccg ggatcgacat
      181 cagttggcgc acgcccgcgg ggatctgggc ccgcagctct tccagggcgg ccaccgagcc
      241 ggtctccagg tactcgtgga tcttctcgcc gatcgacctt cccacgttgg ggatggccag
      301 gatccccttc aggtcgagct cgaccaggtc ggcgtggtac ccgcccaccg cccgagccgc
      361 cttctcgtac gcgcgcggct tgaaggggtc ctccgacagg atcgacagca ggtcggcgta
      421 ttcgaggagg accgcctcca ccgtttcgtt ggttcgaggc atggttcgcc tcctcgcatc
      481 cccttactcg agaccgagga gccagagcag gacggccttc tgggtatgca gccggttctc
      541 ggcctgctcc cacaccgcgg aacgcggccc gtcgatcacc tcgtcggtga tctcctgccc
      601 ccggtgggcc ggcaggcagt gcaggatcgt gacgtcgtcc ttcgccagct ccaccagggc
      661 ctggttcagc tggtacgcgg ggaagaccag cgcccgctcc tcggcctcgt cctcctggcc
      721 catgctggcc cagacgtccg tgtagagcac gtcggcaccg cgacaggccg ccgcggcatc
      781 gttcaggacc tccaccgagc cgcccgtctc ctgggcgatc tcgcgggccc gatgcaccac
      841 ctgcgggatc ggctcgaagc ccggaggggt cgccacccgg acatgcatgg cgaccttcgc
      901 cccggcgagg agcagcgaat gggtcacgtt gttcccgtcg ccgaggtaca cgagcacccg
      961 gccgcccgga ccgccccgag cctcgcgaac ggtgagcagg tccgccagtg cctgacaggg
     1021 gtgctcgaag tccgacagcg cgttgaccac ggggacgctt gccgcggccg ccagctgctc
     1081 gagccgctct tgctcgaagg tccggagcac gatcgcgtcg acgtaccggg agagcacccg
     1141 gccggtgtcc tcgatcgtct ccccccgtcc cagctggagc tcagccgtcg acagggccac
     1201 gggatgacct ccggtgccgg cgatggccac ctcgaaggac acccgtgtgc gcgttgaagg
     1261 cttctcgaag atcagcgcga tggccttgcg gtgcaggcga tccgcgtaat cctccggcgt
     1321 ggccttgacc ttcgcggcca ggtccaggat gcggtcgagc tcgcccggcg tcaggtcgtc
     1381 gatcgaaagg aagtcgcgct ccatcggtcg aagtctatcc gtccgcgcac cccggcgcgg
     1441 cgtccggctg ggaccggcgc tacccggcgc tacccggcgc tatccggcgc ggagctcggc
     1501 gcccaggtcc cgtcccaggc gctccacgat ctgggccacg gcggcgtccg cctcggccgc
     1561 ggtcagggtg cggtcctgcg cccgcaggtc cacggagaac gccaggctct tccggccctc
     1621 gggcaggggc ggaccctggt ggacatcaaa gagcacgcag ccgccgagca ggtcccccgc
     1681 ggcctcctcc agggccgcct gcacgccgcc ggccggcgtg tcctccgcca ccatgaacgc
     1741 caggtcccgc cggaccggag ggaagcgggg gaggtccagg accgcgagct cggagcccgc
     1801 gcggcccatc aacgcctcca ggtcgagttc gcccacggtg acgcgccccg cgaggtccag
     1861 actcgccgcg gccctcggat gcagctcgcc gaaggccccc accacctgcg gtccgatccg
     1921 cacctcggcc gagcgtcccg gatggaacgg gtcgcccggc gggtcgccga gcgcccagcc
     1981 ccggaccccc aggtcggcca gcacggcctc caccacgccc ttgccgtcga agacatcgaa
     2041 cgagcgctcg ggctcggccc agccctgcga ggcgcgaccg gccagcacga agcccgcggc
     2101 gcgctcctcc cggacgtcct taccccccga tcggaaaacc gccccgatct cgaacaacgc
     2161 agcgccccgc acatgccggt tgaggttgcg ccgcagggcc ttgagcagcc ccggcgccat
     2221 cctgggacgc aggtagccct cgtcggcctg cagggggttc gtcaccggca cggcgcccgg
     2281 atccccgaac agcgccaaat ccgtgtcgga gacgaagggc acctggcgta cctcccggag
     2341 cccggccctc accaggctcc gccggacccg cccgatgaag gcgtaccctg cgggcaagcc
     2401 ccccggctgg cgagccgccg gaaggctgga gccgacccgc tcgtagccct ggacgcgcac
     2461 gatctcctcg atcaggtcga cctcgcgctc cacgtccacg cggtagccgg gcacctccac
     2521 ctccacccgg tcctcgtcca cgacccgcac cggcatccgc aggcggtcga acacctc
//
LOCUS       DASQQQ010000255         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_76701, whole genome shotgun sequence.
ACCESSION   DASQQQ010000255 DASQQQ010000000
VERSION     DASQQQ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76701"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VE646_09715"
     CDS             <1..382
                     /locus_tag="VE646_09715"
                     /inference="COORDINATES: protein motif:HMM:NF015970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HZD80300.1"
                     /translation="TCFIGLFVVAHGWVTPGGGFQGGVALAAVPALAMLAGRHVRVRR
                     FRTVPLLDLVEGSAIGAYVVVGGIGALAGSGFLAHVLPLGTPGTLASAGTIVLLNGLV
                     GVAVATGIVLLVAEFLEQTLLRGG"
     gene            379..726
                     /locus_tag="VE646_09720"
     CDS             379..726
                     /locus_tag="VE646_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HZD80301.1"
                     /translation="MMDVLPYAVAAWLFVVGIYGMATSRHLVHLVVCLSVLQSSTYVL
                     LLAVGFRAGATAPAFSNVPQGTPAVDPVVQALVLTDIVVQVTVTALLLALAVQEHKRF
                     GELDPEELRALRG"
     gene            728..>2017
                     /locus_tag="VE646_09725"
     CDS             728..>2017
                     /locus_tag="VE646_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HZD80302.1"
                     /translation="MDPLAPVPVVVPLGAAAVLAAGTTLLRRRVADAISVGTAAGTAG
                     LATWLLVRAGPGPIEYWFGGWAPRGGSVLGVALAVDRLGAGLAAFVAVLVTAALVFSW
                     RYFDTVGTLFHVLLLVFLAAATGFALAGDLFTMFVLFEVLSVSAYALTGYRIEHRAPL
                     QAGLNFAVVNSLGALAVLIGIALLYARTGWLNLAKIGQALTGAPADGLVVVALAFLLG
                     GFLVKAAAVPVHFWLGDAYAVAPTPVGVVFAGALTELGIFGATRVYWTVFSGVRGDHD
                     ALGTVLLVVGVATAAVAAIMAFEQRHLKRLIAFVTISHSGIMLAGVSTLDAGGLTGAS
                     LYAVADGAVKAALFLAIGILLHRLGSVDEDDLRGRGRGMPVVAGVFVLAGLGLAGLPP
                     FGTEVGKLLMDHAASAAGRGWVPPLVAAVSVLTGGAVL"
BASE COUNT          184 a          691 c          779 g          363 t
ORIGIN      
        1 cacctgcttc atcggcctct tcgtggtggc gcacgggtgg gtgacgcccg gcggcgggtt
       61 ccagggcggc gtggcgctgg ccgcggtccc cgcgctggcg atgctggcgg ggcgccacgt
      121 tcgcgtgaga cggttccgga ccgtccccct cctggatctc gtggaggggt cggcgatcgg
      181 cgcctacgtg gtggtcgggg gtatcggggc gctggcgggc tccgggttcc tggcgcacgt
      241 cctgccgctg gggacgcccg gaaccctcgc gtcggcgggg acgatcgtgc tgctcaacgg
      301 cctggtcggt gtggcggtcg cgaccgggat cgtgctcctg gtcgcggagt tcctggagca
      361 gacgctcctg cggggtggct gatggacgtc ctgccgtacg cggtggcggc ctggctcttc
      421 gtggtgggga tctacggcat ggcgacgagc cgccacctcg tgcacctcgt cgtgtgcctg
      481 tcggtgctcc agtcgtccac gtacgtcctc ctgctcgccg tcgggttccg cgcgggggcc
      541 accgctcccg cgttctcgaa cgtcccccaa gggacgcccg cggtggaccc ggtggtccag
      601 gcgctcgtgc tgaccgacat cgtggtccag gtgacggtca cggcgctcct cctggcgctc
      661 gcggttcagg agcacaagcg attcggggag ctggaccccg aggagctgcg tgccctgcga
      721 ggctagggtg gacccgctcg cccccgttcc cgtcgtcgtg ccgctcggcg cggcggctgt
      781 cctcgcggcc gggacgaccc tgctccggcg ccgggtggcc gacgcgatct cggtggggac
      841 ggccgccggc acggccgggc tcgccacctg gctgctggtc cgggcgggac ccggaccgat
      901 cgagtactgg ttcggcggct gggcccctcg cggcggcagc gtcctggggg tggcactcgc
      961 ggtcgaccgc ctcggggcgg gactggcggc gttcgtggcc gtgctcgtga ccgccgcgct
     1021 ggtcttctcg tggcgctact tcgacacggt cgggacgctc ttccacgtcc tcctgctcgt
     1081 gttcctcgcg gcggcgacgg gcttcgcgct tgcgggcgac ctgttcacga tgttcgttct
     1141 cttcgaggtc ctgagcgtct cggcgtacgc tctgaccggg taccggatcg agcaccgggc
     1201 cccgctccag gctggtctga acttcgccgt ggtgaacagc ctcggcgcgc tcgccgtcct
     1261 gatcggcatc gccctcctgt acgcgcggac gggatggctg aacctcgcga agatcgggca
     1321 ggcgctgacc ggagcgcccg ccgacgggct cgtcgtggtc gcgctggcgt tcctgctggg
     1381 gggcttcctg gtcaaggcgg cggcggtccc cgtccacttc tggctgggcg acgcctacgc
     1441 ggttgcaccg acgcccgtgg gcgtggtgtt cgcgggcgcc ctaaccgagc tggggatctt
     1501 cggggccacc cgcgtctact ggacggtgtt ctccggggtg cggggcgacc acgacgccct
     1561 cgggacggtg ctccttgtgg tgggggtcgc gaccgcggcg gtcgccgcga tcatggcctt
     1621 cgagcagcgg cacctgaagc ggctcatcgc gttcgtcacg atctcccact ctgggatcat
     1681 gctggcgggc gtctctaccc tcgacgcggg gggcctcacc ggagcgtcgc tctacgcggt
     1741 ggccgacggg gcggtgaagg cggcgctctt cctcgcgatc ggcatcctcc tgcacaggct
     1801 cggctcggtc gacgaggatg acctgcgggg cagggggcga ggcatgccgg tggtggcggg
     1861 ggtcttcgtg ctggccgggc tcgggctggc cgggctgcca cccttcggca ccgaggtggg
     1921 gaagctcctg atggatcacg ccgcttccgc tgccgggcgc gggtgggtgc caccgttggt
     1981 cgccgcggtc tcggtgctca ccggcggcgc cgtcttg
//
LOCUS       DASQQQ010000256         3071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_130323, whole genome shotgun sequence.
ACCESSION   DASQQQ010000256 DASQQQ010000000
VERSION     DASQQQ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130323"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VE646_09730"
     CDS             complement(<1..541)
                     /locus_tag="VE646_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZD80303.1"
                     /translation="MSVAPASLTPSFPELGVSSTVDRVLSERGMSSPFPIQALVIPDA
                     LEGHDVLGRSKTGSGKTLAFAVPIVERLRAEDPRPAALVLVPTRELAAQVGEDVEPIA
                     RARGLGVAVVHGGGGPRAPQRGRRREGGGHKPSAAHVVIATPGRLNDLVDRGALGLGT
                     IRILVLDEADRMLDMGFQPQ"
     gene            complement(689..1342)
                     /locus_tag="VE646_09735"
     CDS             complement(689..1342)
                     /locus_tag="VE646_09735"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HZD80304.1"
                     /translation="MGSLDAVSLAPKPRLRGRIHEVAFFCSIPAALALVLIARGPAAR
                     IASAVYALSLVGVFGVSASYHRGRWSSAARRRMKRVDHSMIYVLIAGSYTPVSLLVLD
                     GPVAAILLAAVWMGASVGVALKITRIDGFRAATGGLYVGLGWLVLVVMPQLIRGMTAA
                     EVALLLTGGAFYTLGAIVFAAKRPDPRPSVFGFHEVWHALMVTAAACHFAMIALLVR"
     gene            1376..>3071
                     /gene="ade"
                     /locus_tag="VE646_09740"
     CDS             1376..>3071
                     /gene="ade"
                     /locus_tag="VE646_09740"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HZD80305.1"
                     /translation="MQHGSAGELAHRLAVARGDEPADLVLSGGRVFSVFTGELLDADV
                     AVAGEHVAGLGRYEGRERVDVSGQILLPGLIDGHMHIESTKLPPDQFARAALPWGTTT
                     VVLDPHEIANVFGLRGVRALIGAAEDVPLDMFVMASSCVPASPFESGGATVDADDIEA
                     FLRDEPRALGIAEMMDFPALVAGQPETLAKVSASGGRQIDGHAPGLSGRALNAYLAAG
                     VRSDHECTTYEEALEKRRLGMWIMIREGSAARNLEALLPLVLEHGPANCLLCTDDREP
                     DHLVDEGHVNDIMRKAVAMGCPPADAVVMGTLNAARYHRLYEYGAIAPGRVADIVAVE
                     DLSRFRPLQVWKRGRLVAEAGTAREVPARPMPDWMRGSVRVRPLRADDFSVHVDGQVR
                     VIGVDPGQIVTRSLVDQPGREGGSAVADPSRDLAKIAVIERHRGTGRIGLGFVRGFGL
                     RRGALASTHAHDAHNVVVVGVDDDDMAAAANRLAQTGGGQVALASEEVLGEVACPFGG
                     LLSDRPAEEVAAAVRGLDAAATGLGVTLPAPFMAMSFLALSVVPELKLTDRGLVDVD"
BASE COUNT          466 a         1072 c         1126 g          407 t
ORIGIN      
        1 cctgcggctg gaaccccatg tccagcatcc gatccgcctc gtccaggacc aggatccgga
       61 tggtgccgag cccgagcgcg ccacggtcca ccaggtcgtt cagcctgccc ggggtggcga
      121 tcaccacgtg cgcggcggac ggcttgtgcc ctccgccctc ccggcgacga ccgcgctggg
      181 gcgcacgagg accgccgccg ccgtggacca ccgccacgcc cagaccccgg gcccgtgcga
      241 tcggctcgac gtcctcgccg acctgagcgg ccagctcccg ggtggggacc aggaccaggg
      301 cggcgggcct gggatcctcg gctcggagcc gctccacgat ggggacggcg aaggccaggg
      361 tcttgcccga gccggtcttc gatcggccga gcacgtcgtg gccctccagg gcgtcgggga
      421 tcaccagggc ctggatcggg aacgggctgg acatgccccg ctccgagagg acacgatcca
      481 cggtggacga gacgccgagc tcggggaagg acggcgtcag ggaagcgggt gcgacggaca
      541 tgttcactcc ttctgccacg gatacccgcg gcgggtccgg gggaggttct gcgtgacccc
      601 gagaccgccg gaaggaggaa cgaggaagcg aaacctcacg ggacggcatc caccgtccgt
      661 acctggagcg tatcaggaag gggagcgctc agcggaccag gagggcgatc atggcgaagt
      721 ggcacgcggc ggcggtcacc atgagcgcgt gccacacctc gtggaacccg aagaccgacg
      781 ggcgggggtc cggccgcttg gcggcgaaga cgatcgcccc cagcgtgtag aacgcacctc
      841 cggtcaggag cagggccacc tccgcggccg tcatgccccg gatcagctgc ggcatcacga
      901 ccagtaccag ccaccccagc cccacgtata ggccgccggt ggccgcccgg aacccgtcga
      961 tccgcgtgat cttgagggcc acgccgaccg aggcccccat ccacacggcg gccagcagga
     1021 tcgccgctac ggggccgtcc agcacgagca gggacaccgg cgtgtagctc ccggcgatga
     1081 ggacatagat catcgaatgg tccacgcgct tcatccgccg acgagcggcc gaggaccacc
     1141 gccctcggtg ataggaggcg ctcacgccga agaccccgac caggctcagc gcgtaaacgg
     1201 ccgaggcgat ccgcgccgcc gggccccgcg cgatgagcac cagggcgagc gcggccggga
     1261 tcgaacagaa gaacgccacc tcgtggatcc ggccgcggag ccgaggcttc ggagcaagag
     1321 acaccgcgtc caaggacccc atgctacgtc cgacggggcg cgatacgctt cctggatgca
     1381 gcacggctcc gccggcgaac tcgcgcaccg cctggccgtc gctcgcggcg acgagccggc
     1441 cgacctggtg ctgtccggcg gacgggtctt ctccgtgttc acgggcgagt tgctcgatgc
     1501 cgacgtggcg gtggccggcg aacacgtcgc cgggctgggt cgctacgagg gccgggagcg
     1561 ggtcgatgtg tcgggccaga tcctgctccc cggcctgatc gacggccaca tgcacatcga
     1621 gtccacgaag ctcccgcccg atcagttcgc tcgagcggcc ctgccctggg gcactaccac
     1681 ggtcgtgctc gacccgcacg agatcgcgaa cgtcttcggc ctgcgaggcg tccgagccct
     1741 gatcggggcc gccgaggatg tccccctaga catgttcgtg atggcctctt cgtgcgtccc
     1801 cgcgagcccc ttcgagtccg gcggcgccac cgtggacgcc gacgacatcg aggccttcct
     1861 gcgggacgag ccccgggccc tgggcatcgc cgagatgatg gatttccccg ccctggtggc
     1921 cggccagccg gagaccctgg cgaaggtctc ggcatcgggg ggacgccaga tcgacgggca
     1981 tgctccggga ctatcgggcc gggccctcaa cgcctacctg gcggccggcg tccgcagcga
     2041 ccacgaatgc accacgtacg aggaagcgct ggagaagcgc cggctgggga tgtggatcat
     2101 gatccgtgag gggtccgcgg ccaggaacct ggaggcgctc ctgccgctcg tgctggagca
     2161 cggtccggcc aactgtctgc tctgcaccga cgaccgcgaa cccgaccacc tggtcgacga
     2221 ggggcacgtc aacgacatca tgcgcaaggc ggtggcgatg ggctgtccgc cggccgatgc
     2281 cgtggtgatg gggaccctga atgcggcccg gtaccaccgc ttgtacgagt acggcgccat
     2341 cgcgccgggc cgggtcgctg acatcgtggc ggtggaggac ctcagccggt tccgtccgtt
     2401 acaggtctgg aagcggggcc ggttggtggc cgaggccgga acggcgcgcg aggtccccgc
     2461 gcggcccatg cccgactgga tgcggggcag cgtgcgcgtg cggccgctcc gggccgatga
     2521 cttctcggtc cacgtggacg gacaggtgcg ggtgatcggt gtggatcccg ggcagatcgt
     2581 cacgcgttcg ctggtggacc agcccgggcg ggaggggggc tcggcggtgg ccgatccctc
     2641 ccgggacctg gccaagatcg ccgtgatcga acgccaccgt gggaccggcc ggatcgggct
     2701 cggcttcgtc cgagggttcg ggcttcggcg gggggcactc gcctccacgc acgcccacga
     2761 cgcccacaac gtggtggtgg tcggcgttga cgacgacgac atggcggcgg ccgcgaaccg
     2821 cctggcccag accggaggcg gacaggtcgc cctcgcgagc gaagaggtgc tcggcgaggt
     2881 ggcctgtccc ttcggcggac tgctgtcgga tcggccggcg gaggaggtgg ccgccgcggt
     2941 ccgggggctg gatgctgcgg ccacgggcct cggtgtcacc ctgccggcgc cgttcatggc
     3001 tatgtcgttc ctggcgctgt ccgtggtgcc cgagctcaag ctgaccgacc gcggcctggt
     3061 cgacgtcgac c
//
LOCUS       DASQQQ010000257        13536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_231533, whole genome shotgun sequence.
ACCESSION   DASQQQ010000257 DASQQQ010000000
VERSION     DASQQQ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13536
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231533"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VE646_09745"
     CDS             <1..648
                     /locus_tag="VE646_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HZD80306.1"
                     /translation="TGVSVVLTRGEDRAILTAPGTIAALRRDLVQPDLVRTARHVHVA
                     SYFLQLDLRGELLELFRDAHGAGVTTSIDPNWDPTEGWDGGLLPLLEHTDLFFPNAAE
                     ARAITGIEEASVAAQALAERGPVVAMKFGRDGGLVVAGTEAVRCRSVPIDAKDTTGAG
                     DSFDAGFLAGRLEGWDLDRCLALAVACGALSTRALGGTGGQPTMEEALAAMEVVP"
     gene            645..1664
                     /locus_tag="VE646_09750"
     CDS             645..1664
                     /locus_tag="VE646_09750"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HZD80307.1"
                     /translation="MIVCVAASPSVDKLFEVERVRIGAIHRPASFVSVPGGKGLNVAR
                     AAHALGAEVRATGILAGHAGRWIEGALADEGVPGTFAWVEGETRASLSVADATDRGLT
                     EFYERGTDIGPHGWARLEDVVRGLLPGAGWMTISGFVPPGAPDDGYARLVKEAHAMGV
                     RVALDTRDDPLLMGIAAGPDVVKVNAEEAGALLGMRLEALEEAVHAATEIRWRLDGSD
                     RAAIITRGAEGAVVVTPQGDVLQGRLYERGRYPVGSGDAFLGGLVTGLDRGEPWQQAL
                     RLALGGAAANAMVPGAGRLDPATAERLSSAAVVELLEGDGTRLRTAPTPGAPPAGDEP
                     SPGSR"
     gene            complement(1600..2484)
                     /locus_tag="VE646_09755"
     CDS             complement(1600..2484)
                     /locus_tag="VE646_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006444910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80308.1"
                     /translation="MSRVDPRDLERWCRIPVDELAAYRPRVPFRLVADAREMALLMAK
                     ELVDVVERNNEENQPTRAIVPCGPSGWYAPFRERVNARSVSLTHLSVFHMDECLDWQG
                     RQLPVGHPYNFRTFMEREFYGGIRPELAVPEPQRHWLLPATAEAVAAAIAEGPVDITV
                     GGWGQDGHVAYNQARRDPYRALTVEELADSSIRIQENSADTVIALANRTFGSAYQFVP
                     QMSITLGMRECLSAAQIRVYSDTGAWKQTAFRVALFSEPTAEYPITLLQRHPDAIVTA
                     TRETARHPLEEHPEWELF"
     gene            complement(2481..3182)
                     /locus_tag="VE646_09760"
     CDS             complement(2481..3182)
                     /locus_tag="VE646_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZD80309.1"
                     /translation="MDFVSLREEVWWANRQLPERGLVAAHSGNASGLHPDTGLVLIKP
                     SGVDYDRLRPEDLAVVTLDGHPAPADAIPDRIVSSLRPSVDTPHHLALFRADPGIGGV
                     VHTHSSFATAWAAVARSVPCALTAMADEFGGEIPCAPYADNEGDHIGEAILRHRTRAP
                     GVLLANHGVFAFGTSARAALKAATMIEDAAKTMWLARQLGEPTAIDPDEIEKWWTRYH
                     ATYGQEPRSELGSPP"
     gene            complement(3195..4307)
                     /locus_tag="VE646_09765"
     CDS             complement(3195..4307)
                     /locus_tag="VE646_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HZD80310.1"
                     /translation="MRLAPAPSAGLRAVRPEPFVRLVGVGGIGSGIVMALEGDRTLGR
                     DESRGARLLDARDRCKLHIVAHHVARLLAADPSGRPFHVVPVGRVGDDQAGRALVAEM
                     RSAGMDTSRVEAVPGRPTLFSVCFQYPDGAGGNLTTVDSAASALGRSEVDAVAPLVEE
                     AGPRGIVLAAPEVPLAVRRRLLELGTQVGAFRVASFLTAEVREAQGSGLLEMVDLLAM
                     NREEAAVLAGPLDPHDPEPFLGRVAGALDGERGGMRIVVSAGALGAFGFEEGCWVHRP
                     APSVEVAGTAGAGDALLGGIVAALAVGIPLLAPTAGVDPPAKLADALDLGVLLAACAV
                     TSPHTIPPDADLRSVLAFAEALGLSPRPPLAACLHE"
     gene            complement(4332..5321)
                     /locus_tag="VE646_09770"
     CDS             complement(4332..5321)
                     /locus_tag="VE646_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZD80311.1"
                     /translation="MYLGPRRMELQEAPDPTPGAGEVVFRTEAAAVCGSDLHGFREAS
                     PRRIPPLVMGHEGVGTVPAVGDGVDPGLLDRRVVLKPILACGACEPCRAGRTNLCLNG
                     RLVGRELTGAFAERFVVPEEAVAPISPQVPADLATLTEPLANAVHVTSRSVRPHDDVL
                     VIGAGPIGVLMARMALERGARRAFVTDPIRTRRELARAQGATPLEGEDPEAGLREATG
                     DRGVDVVIDAAGLEATWALGLRVVRPGGRIEAVGLGSPGGRIDFFAVAGKEITITGSF
                     AWTDEDFSRALDAIEGGLIDTGGWFTTMPLAEGQRAFEELVDTTDRFKVVLVP"
     gene            complement(5330..6166)
                     /locus_tag="VE646_09775"
     CDS             complement(5330..6166)
                     /locus_tag="VE646_09775"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZD80312.1"
                     /translation="MPTPIERLQQIEIGPASAAVWWLGQSGFIVRGGGATILLDPFLS
                     PHEGRAYESALAPEDAAGVDLVLCSHEHVDHFDADSAPGIAAASPAAVFVVPTPIVDM
                     VTEVGIPADRVVGVQPGETTELRGVTIRPVPAMHGVTMEDAYGFGEALSGGLIRFVGF
                     VVDLGGLRLYHAGDTIHYEGMVPTLRELAVDVAMLPVNGRDPERESRDIVGNLSEREA
                     AWLAAEIEAHVLIPMHHDLFPYNRGHPEHVVQSARDEHPGASVLVPHRDRPFVLSAVR
                     PR"
     gene            complement(6177..6929)
                     /locus_tag="VE646_09780"
     CDS             complement(6177..6929)
                     /locus_tag="VE646_09780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZD80313.1"
                     /translation="MRGLEGKGVLVSGASRGIGEAAARRFLEEGCRVFICARGAPGVE
                     QAVRELLTLGEAAGMACDVSRSEDVDLLVEEAERFLGGIDVLANNAGVAWQEPFLETT
                     LEHWDEVLDINLRGMFLVAQKVAQKMVARGRGGSIVNMSSTNAFEGEAGYAHYNASKG
                     AITMLTRTMATELGVHGIRVNALCPGKIFTPLQGEAEDPEYTAWYEREKIPLGRSGAV
                     EEVAAAYAFLASDEATFITGEMLVVDGGQLAM"
     gene            complement(6929..8050)
                     /locus_tag="VE646_09785"
     CDS             complement(6929..8050)
                     /locus_tag="VE646_09785"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZD80314.1"
                     /translation="MSDAIPHLDAISDLDTPSLLIDLDTLEDNITEMQEAVRASGVAL
                     RPHAKTHKCPDIARMQLEAGARGITVAKLGEAEVMVDAGIDDVLIAYPIVGEQKLSRL
                     RELAARAQIAVTTDSVEVAEGLGRVGKDLGREMPVLVEVDTGLHRLGRPPGASTVALV
                     QELSELPGVEIVGLLTHAGHAYRSSSREELQAVAEREGRDLVETAERCAEVGIPLRRI
                     SVGSTPTARIVAGVPGVTEIRPGTYVFNDVQQMRLGVATEERCALGVLTTVVAHPSDE
                     RFVLDAGTKAFTSDGADGPPFPGRGVVAGRPELRLDFMNEEHAVGRRTGRDDVRIGER
                     LEVIPLHACATVNMFDRAFGVRDGEVVRTFDIAARGKLR"
     gene            complement(8047..8436)
                     /locus_tag="VE646_09790"
     CDS             complement(8047..8436)
                     /locus_tag="VE646_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009721137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HZD80315.1"
                     /translation="MDERDEVRTEGAPRGTGAYSQAIRAGDFVFISGQGPLDPVSLEV
                     VGTTVEEQTEATLRNLGAIATAAGGSLRDVVKVSAFLSSIDLFPAFNATYERIFDALP
                     RPARTTAGAELRDILVEIDAVLYLPQR"
     gene            8620..9975
                     /locus_tag="VE646_09795"
     CDS             8620..9975
                     /locus_tag="VE646_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014505.1"
                     /note="catalyses the hydrolysis of terminal non-reducing
                     alpha-D-galactose residues in alpha-D-galactosides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HZD80316.1"
                     /translation="MPADGPRIVIVGAGGWVFPLELSRDILSFTGLASGSLVLYDIDH
                     AASERTRGFVERLMAAGKVQATVEVAPDLPTALRGADFVITVFQVGGVEAYAHDVLIP
                     REYGIDQTVGDTLGPGGIFRGLRTVEALRVVAEAMLEICPDAWLLNYANPMSINCWAT
                     DLLGVKIVGLCHSVQGTSELLARELGVPYDEVTFDCAGVNHTAWFTTFRRGGEDLIPR
                     IREVMRARHVDRSIPLLPRSDDPYESIERVRAELMMLTGYFHTESSHHASEYWAWFRK
                     TPELRQEYLDRRWDYLAICQSADASYTNEDLVEEAERGGLHHGGEFAAPIMDSIVTGA
                     TRVVYGNVRNGGSIENLAPEACVEVACAVDGNGIRPLRYGMLPPACAALNQLQIAVQG
                     LTVEAAMTGDRDLAHAAVAIDPLTSAVLALPRIHEMVDRMLEAEAPWLPRFEPRATAR
                     A"
     gene            9991..11097
                     /locus_tag="VE646_09800"
     CDS             9991..11097
                     /locus_tag="VE646_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZD80317.1"
                     /translation="MPAGSSRRELVDLPLHAYRPVPRIRLPQHPVHRARFPAIDAHNH
                     LGRWLSEWLDRPGEWAVRSVPHLLDLMDRCNLRAIVNLDGRWGEELEANLDRYDRAHP
                     GRFVTFCHVDWAAASERELGRAAAASLRASVAAGAKGLKVWKDLGLHVRDAGGELVLP
                     DDRRLAPLWETAAELRVPVFIHTADPVAFFDPVDERNERLEQLLAHPEWSFADPSFPR
                     FERLIEAFEGLVSAQPDTTFVGVHVAGYAEDLSWVGRMLGEHPNLHIDIAARVAELGR
                     QPRAARELVLAYPDRILFGIDEFPPDPANYAIYLRFLETADEHFPHSAEEIPLMGRWA
                     ISGLGLPDEVLRKVYADNALRVVPGLDTSEGGTS"
     gene            11217..12632
                     /locus_tag="VE646_09805"
     CDS             11217..12632
                     /locus_tag="VE646_09805"
                     /inference="COORDINATES: protein motif:HMM:NF015500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HZD80318.1"
                     /translation="MRLTVLHPTGIVDATGTHSGNVGALAKRDQSGARDDPSRYVWLR
                     GRGRAYGGYRNYTLPGAVSPADVTTITLTANFRGPIARANAWTWAAYDREEGRWVRLG
                     DQRHCGGDAGTAQWPCDDLQRRPWKYVRYNVIPPSGEDLSDFIDPSTGQMRIRLRASR
                     SGRARLDWEALEVYSNPGPAEARWTPPVGARWQWQLEGRAGHHLSTGGIAVGICQPPF
                     GGATCVRPDLFDIDLYVDPAIAGRFGYRLERPAVKAIHASDRHVIGYVTAGDIERWRP
                     DYEQFIDFDRRCDGCLRGKPFSKRFPDEYWANLNKGQGQFAFMLQMLRARTDRVAATG
                     FDGIEYDIVDTYDQGRQTTGFKISAQRQLAYNRALARMAHADGLSVALKNDGGQIQEL
                     LHSFDFAINEQCFQYDECAGGGYPSPGWRAFIEAGKAVFQAEYRLQPRDFCRKANRWD
                     FSSIVKSRDFSLYALPWTPCR"
     gene            complement(12652..>13536)
                     /locus_tag="VE646_09810"
     CDS             complement(12652..>13536)
                     /locus_tag="VE646_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZD80319.1"
                     /translation="DIAMVFQNYALYPHMTVYRNLAFGLRQRHVGKAEIDRRVREVSA
                     MLGLDELLKRRPAQLSGGQRQRVAMGRALVREPSAFLLDEPLSNLDAKLRVQMRAELK
                     RLHARLGVTTIYVTHDQVEAMTLGDRIAVMSNGKLQQIGDPQEVYDRPANLFVAGFIG
                     SPPMNLIRGRARSGTIVAGDLTFERPGVPDGDVAVGMRPEAVSASPDGLPTFDFLVDV
                     VEPLGDEVVVHGSAGATMVETGAEEEEVPTMIEGSRAPITVRFDPDVRMTPGERVRLG
                     IRPERIHLFDLRTGAAIR"
BASE COUNT         1937 a         4786 c         4867 g         1946 t
ORIGIN      
        1 accggggtct cggtggtgct gacgaggggc gaggaccgcg cgatcctcac cgcgccgggg
       61 accatcgccg cgctgcggcg ggatctggtg caacccgacc tggttcggac cgcgcgacac
      121 gtccacgtcg cttcctactt cctgcagctc gacctccgcg gcgagctcct ggaactgttc
      181 cgcgatgccc atggggcggg cgtgacgacg tcgatcgatc ccaactggga tccaaccgaa
      241 ggatgggacg gcggcttgct gccgctcctg gaacacaccg acctgttctt cccgaacgcc
      301 gcggaggcac gggccatcac cggcatcgag gaagcgagtg tcgcggctca ggcgctcgcc
      361 gagcgggggc cggtggtggc catgaagttc ggccgggatg gtggcctcgt cgttgcgggg
      421 accgaggctg tccggtgtcg gtcggtgccg atcgacgcga aggatacgac cggggccggg
      481 gacagcttcg acgccgggtt cctggccggg cggctggaag gctgggacct tgaccgatgt
      541 ctggcgctcg ccgtggcgtg cggggcgctc tcgacgcgcg cgctgggcgg tacgggcggc
      601 cagcccacga tggaggaggc gctggccgcg atggaggtgg tgccgtgatc gtctgcgtgg
      661 cggcgagccc ttcggtggac aagctcttcg aggtggagcg ggtccggatc ggggcgatcc
      721 accgccccgc ctcgttcgtg agcgttcccg gcggcaaggg gctgaacgtg gcgcgagccg
      781 cccacgccct gggggccgag gtgcgggcga ccggcatcct ggccgggcac gccggccggt
      841 ggatcgaggg ggcgctggcc gacgagggcg ttcccggaac gttcgcctgg gtggagggag
      901 agacgcgggc gtcgctctcg gtggccgatg ccacggaccg cgggctgacc gagttctacg
      961 agcgcggcac ggacatcggt ccgcacgggt gggcgcggtt ggaggacgtc gttcgaggcc
     1021 tgctccctgg cgcgggttgg atgacgatct ccgggttcgt gccgcccggc gcaccggacg
     1081 acggctacgc ccggctggtg aaggaggccc acgcgatggg cgtccgggtc gcgctcgaca
     1141 cgcgggacga cccgctgctg atgggcatcg ccgccggccc ggacgtggtg aaggtcaacg
     1201 ccgaggaggc cggagccctg ctcgggatga gactggaggc ccttgaggag gcggtgcacg
     1261 cggccaccga gatccgctgg cgcctggatg ggtccgatcg ggccgccatc atcacccgag
     1321 gcgccgaggg ggcggtggtg gtgacgcccc agggggacgt cctccagggg cggctctacg
     1381 agcggggccg gtatccggtg ggcagtggcg acgcgttcct gggcggcctg gtcaccgggc
     1441 tcgaccgagg tgagccgtgg cagcaggccc tccgtctggc gctggggggg gcggcggcca
     1501 acgcgatggt ccccggggcg ggccggctgg atccggccac cgcagagcgc ctctcatcgg
     1561 cggcggtggt ggaactgctg gagggggacg gcacgcggct cagaacagct cccactccgg
     1621 gtgctcctcc agcgggtgac gagccgtctc ccgggtcgcg gtgacgatcg cgtcggggtg
     1681 gcgctggagc agcgtgatcg ggtactcggc tgtgggctcc gagaacaggg ccacgcggaa
     1741 ggccgtctgc ttccaggcgc ccgtgtcgct gtagacgcgg atctgggcgg ccgacagaca
     1801 ctcgcgcatc ccgagggtga tcgacatctg cggcacgaac tggtaggcgg agccgaaggt
     1861 ccggttcgcg agcgcgataa cagtgtccgc actgttctcc tggatccgga tggacgagtc
     1921 ggccagctcc tccacggtga gggctcggta cggatcgcgc cgggcctggt tgtaggccac
     1981 atgaccatcc tggccccacc cacccaccgt gatgtcgacc ggcccttcgg cgatcgccgc
     2041 cgccacggcc tcggcggtcg cggggagcaa ccagtggcgc tgcggctcgg gcaccgcgag
     2101 ctcggggcgg attccgccgt agaactcgcg ctccatgaag gtccggaagt tgtaggggtg
     2161 gccgaccggg agctgccgac cctgccagtc caggcactcg tccatgtgga acaccgacag
     2221 gtgggtcagg gagacgctcc tcgcgttcac acgctcgcgg aagggcgcgt accagccgga
     2281 agggccgcac ggcacgatgg cccgcgtggg ttggttctcc tcgttgttcc gctcgaccac
     2341 gtccacgagc tccttcgcca tcaacagcgc catctcacga gcgtcggcaa cgagccggaa
     2401 cggcacccgg ggacgatagg cggcgagctc gtccaccggg atgcggcacc accgctccag
     2461 gtcgcgcggg tcgacgcggc tcacggcggg ctcccgagct cggaccgagg ttcctggccg
     2521 tacgtcgcgt ggtagcgggt ccaccacttc tcgatctcgt ccggatcgat cgcggtgggc
     2581 tcgcccagct gccgggccag ccacatcgtc ttcgcggcgt cctcgatcat cgtggccgcc
     2641 ttcagcgcgg cccgggcgct cgtgccgaac gcgaacacac cgtggttggc gagcaggaca
     2701 ccgggcgctc gggtccggtg gcgcaggatc gcctccccga tgtgatcgcc ctcgttgtcc
     2761 gcgtaggggg cgcaggggat ctcgcccccg aactcatccg ccatcgccgt gagggcgcag
     2821 gggaccgatc gggccacggc ggcccaggcg gtggcgaagc tcgaatgcgt gtgcaccacg
     2881 ccgccgatcc ccgggtccgc ccggaacagc gccaggtgat gcggggtgtc cacgctgggt
     2941 cgcagggagg agacgatccg gtcggggatc gcgtcggcgg gcgcaggatg cccgtcgagc
     3001 gtgaccaccg ccaggtcctc ggggcgcaac cggtcgtagt ccacgccgct tggcttgatc
     3061 aggaccagcc ccgtgtccgg gtgcaggccg ctggcgttcc ccgagtgcgc cgccaccagc
     3121 ccgcgctcgg gcagctggcg gttggcccac cacacctcct cgcgcaacga gacgaagtcc
     3181 accgttctcc ctcctcactc gtgcaggcag gccgccagcg gtggccgggg cgagagcccc
     3241 agggcttccg cgaaggccag gaccgaccgc agatccgcgt ccggggggat cgtgtgcggc
     3301 gacgtcacgg cgcaggcggc cagcagcacg cccaggtcca gggcgtcggc gagcttcgcc
     3361 ggtggatcga cgcccgccgt gggtgcgagc agcgggatcc ccacggcgag ggcggccacg
     3421 atcccgccca gcagcgcgtc gcccgccccg gcggttccgg ccacctccac cgaaggggcg
     3481 gggcgatgga cccagcagcc ctcctcgaag ccgaaggcgc cgagcgcgcc ggcgctcact
     3541 acgatgcgca tgccccctcg ctccccatcg agggcgccgg ccacccggcc caggaacggt
     3601 tcggggtcgt gcgggtcgag cggaccggcc aggacggccg cctcctcccg gttcatggcc
     3661 aggaggtcga ccatctccaa caggcccgag ccctgcgcct cgcgcacctc ggccgtcagg
     3721 aacgaggcca cccggaaggc cccgacctgg gtgcccagct cgagcaggcg ccggcggacg
     3781 gccaacggca cctcgggcgc cgcgagcacg atcccccgtg gcccggcctc ctcgaccagg
     3841 ggggcaacgg cgtccacctc ggatcgcccg agcgccgagg cggccgagtc caccgtggta
     3901 aggttgccgc ccgctccgtc cggatactgg aagcacacgc tgaacagggt ggggcggccc
     3961 gggaccgcct ccacccggct cgtgtccatg ccggccgatc gcatctccgc caccagcgcc
     4021 cgtccggcct ggtcgtcccc gacccggccc accgggacca cgtggaaggg tcgcccggac
     4081 ggatcggcgg ccagcagccg ggccacgtgg tgcgcgacga tatggagctt gcagcggtcc
     4141 cgggcatcga gcagccgggc gccgcggctc tcgtcccggc ccagggtgcg gtcgccctcg
     4201 agcgccatga cgatcccgct gccgatcccg cccacgccca ccagccgcac gaacggctcg
     4261 ggccggacgg cacgaagacc ggcggatggc gccggcgcga gcctcacggc acgagcctca
     4321 cgtggcgagc ctcacgggac gagcaccacc ttgaaccggt ccgtcgtgtc gacgagctcc
     4381 tcgaacgccc gctggccctc cgccagcggc atggtcgtga accagccgcc cgtgtcgatg
     4441 agaccgccct cgatcgcatc cagggcccgc gagaagtcct catcggtcca ggcgaaggag
     4501 ccggtgatcg tgatctcctt cccggccacg gcgaagaagt cgatccgtcc gccgggcgag
     4561 cccagcccca ccgcctcgat ccgcccgccc gggcgcacca cccgaagacc cagggcccag
     4621 gttgcctcga ggcctgcggc atcgatcacg acgtccacac cccggtcgcc ggtggcctcc
     4681 cggaggcccg cctcggggtc ctcaccctcg agcggtgtcg ctccctgggc ccgggccagc
     4741 tctcgtcgcg tgcggatcgg gtccgtgacg aacgcccggc gggcgccccg ctcgagggcc
     4801 atccgagcca tcagcacccc gatgggaccg gcaccgatca ccagcacgtc gtcgtggggg
     4861 cggacggagc gcgaggtgac gtggacggcg ttcgctagcg gctcggtgag ggtggccagg
     4921 tccgcgggca cctggggcga gatcggcgcc acggcctcct ccgggaccac gaaccgctcg
     4981 gcgaacgctc cggtcagctc ccggcccacc agccgcccgt tcagacacag gttggtccgg
     5041 ccggcccgac acggttcgca ggccccgcag gccaggatcg gtttgagcac cacccgtcgg
     5101 tcgagcagtc cggggtcgac gccgtcgccg acggcgggga ccgttcccac gccctcgtga
     5161 cccatcacga ggggcgggat ccggcggggg ctggcctccc ggaacccgtg gaggtccgag
     5221 ccgcacaccg cagcggcctc ggtccggaag acgacctccc ccgccccagg ggtcggatcg
     5281 ggcgcctcct gcagctccat ccgccggggc cccagataca cgagggccct catcggggtc
     5341 gcaccgccga gaggacgaac ggcctgtccc tgtggggcac cagcaccgag gctccgggat
     5401 gctcgtcccg agcgctctgc accacgtgct ccgggtgccc gcggttgtac gggaacaggt
     5461 cgtggtgcat cgggatcagc acgtgggcct cgatctcggc cgcgagccag gccgcctcgc
     5521 gctcggagag gttgcccacg atgtcgcggg actcgcgctc gggatctcgg ccgttgaccg
     5581 ggagcatcgc gacgtccacg gccagttctc ggagggtggg caccatgccc tcgtagtgga
     5641 tcgtgtcgcc cgcgtggtac aggcggagcc cgccgagatc gaccacgaag cccacgaacc
     5701 ggatcagccc gccagagagg gcctctccga acccgtacgc gtcctccatc gtgacgccat
     5761 gcatcgccgg gaccggtcgg atcgtcacgc cgcggagctc ggtggtctcg ccgggctgga
     5821 cccccaccac ccgatcggcc gggatgccga cctcggtcac catgtcgacg atcggggtgg
     5881 gcacgacgaa cacggcggca ggggaggcgg ccgcgatgcc cggggcgctg tcggcatcga
     5941 agtggtcgac gtgctcgtgc gagcacagga ccaggtccac cccggcggcg tcttccggtg
     6001 cgagcgcgct ctcgtaggcc cggccctcgt gcggcgagag gaaggggtcc aggaggatcg
     6061 tggcaccgcc tccgcggacg atgaacccgg actgaccgag ccaccagacg gcggcgctcg
     6121 ccggaccgat ctcgatctgc tggagccgtt cgatcggtgt tggcatgggg cctccttcac
     6181 atcgcgagct gcccgccgtc caccacgagc atctcgccgg tgatgaacgt cgcctcgtcc
     6241 gaggccaaga acgcgtacgc cgcggccacc tcctccacgg cgcccgagcg cccgagcggg
     6301 atcttctcgc gctcgtacca cgccgtgtac tcggggtcct ccgcttcccc ttggagcggg
     6361 gtgaagatct tgcccgggca cagcgcgttc acgcggatcc cgtggacacc aagctcggtg
     6421 gccatggtgc gggtgagcat ggtgatggcg cccttcgacg cgttgtagtg cgcgtagccc
     6481 gcctccccct cgaaggcgtt ggtcgaggac atgttcacga tcgagccgcc ccgcccgcgg
     6541 gccaccatct tctgggccac cttctgggcc accaggaaca tcccgcgcag gttgatgtcg
     6601 agcacctcgt cccagtgctc cagcgtggtc tccaggaagg gctcctgcca ggccacgccg
     6661 gcgttgttgg ccagcacgtc gatgccgccc aggaaccgct cggcctcctc gaccagcagg
     6721 tcgacgtcct cggagcggct cacgtcgcag gccatgccgg cggcctcccc gagcgtgagc
     6781 agctcgcgca cggcctgctc cacgccgggg gcgccgcggg cacagatgaa gaccctgcag
     6841 ccttcctcca ggaaccgacg ggcggccgct tcgccgatcc cgcggctcgc cccgctcacc
     6901 aggaccccct ttccctccag ccctcgcatc agcgcaactt cccccgcgcg gcgatgtcga
     6961 aggtccggac gacctcgccg tcccgaacac cgaacgctcg atcgaacatg ttcaccgtgg
     7021 cgcacgcgtg cagcgggatc acctccaacc gctccccgat ccgtacgtcg tcccgcccgg
     7081 tgcggcgccc cacggcgtgc tcctcgttca tgaagtccag gcggagctcg ggccggccgg
     7141 ccaccacgcc gcgtccgggg aagggagggc cgtccgcgcc gtcggaggtg aaggccttgg
     7201 tccccgcgtc gagcacgaac cgctcgtcgc tggggtgggc caccacggtg gtgagcacgc
     7261 ccagcgcgca tcgctcctcg gtggccacgc ccaggcgcat ctgctggacg tcgttgaaga
     7321 cgtacgtgcc gggccggatc tcggtcaccc cgggcacgcc cgccacgatg cgggcggtgg
     7381 gcgtggagcc cacgctgatc cgccggagcg ggatcccgac ctcggcgcac cgctcggccg
     7441 tctccaccag gtcacgcccc tcgcgctcgg ccacggcctg cagctcctcg cggctcgagc
     7501 tccggtaggc gtggccggcg tgggtgagaa ggcccacgat ctccaccccg ggcagttccg
     7561 acagctcctg caccagggcg acggtggagg ctccgggcgg gcgtcccagc cgatgcagcc
     7621 ccgtgtcgac ctccaccagg acgggcatct cgcggcccag gtccttgccc acgcggccca
     7681 ggccctcggc cacctcgacc gaatcggtgg tcacggcgat ctgggcgcgc gcggccagct
     7741 cgcgaaggcg agagagcttt tgctcgccca cgatcgggta ggcgatcagc acgtcgtcga
     7801 tgccggcgtc caccatcacc tcggcctcgc cgagcttggc caccgtgatg ccgcgagcgc
     7861 cggcctcgag ctgcatccgg gcgatgtccg ggcacttgtg cgtcttcgcg tggggacgca
     7921 gggcgacccc agaggctcgg acggcctcct gcatctcggt gatgttgtct tccagcgtgt
     7981 ccaggtcgat cagcagggag ggcgtatcca ggtcggagat cgcgtcgagg tgggggatcg
     8041 cgtcgctcac cgctggggca ggtacaggac ggcatcgatc tcgaccagga tgtcccgcag
     8101 ctcggcgccg gcggtggtcc gggccggcct ggggagcgcg tcgaagatcc gctcgtacgt
     8161 ggcgttgaag gcggggaaca gatcgatgga ggacaggaac gcggagacct tgaccacgtc
     8221 acggagggac ccgcccgcgg cggtcgcgat ggcccccagg ttccgaagcg tcgcctccgt
     8281 ctgctcctcc accgtggtac ccacgacctc gaggctcacc gggtccagcg gtccctggcc
     8341 cgagatgaac acgaagtcgc cggcccggat ggcctgagaa taggcaccgg tccccctggg
     8401 tgcgccctcc gtgcggacct cgtcccgttc gtccatcggc tcctcccacc cgcggtccga
     8461 ccgctcggac gtcgcccgga ttctagtacc gaggccttgc ggtgcgcata tctgtcgctt
     8521 ctgccgcgcg ttccatcacg aacgtgcagg caggctaccg acgggagtag gattcggacc
     8581 gtcgagcagg ggcgaggatc cggaacgaag gaggcggcca tgcccgcgga cgggcccagg
     8641 atcgtgatcg tcggtgccgg ggggtgggtg ttcccgctgg agctgtcccg ggacatcctg
     8701 tccttcaccg ggctggcctc gggctcgctc gtcctgtacg acatcgatca cgcggcctcc
     8761 gagcggacgc gcggcttcgt ggagcggctg atggccgcgg ggaaggtgca ggccaccgtg
     8821 gaggtggcgc ccgatcttcc gacggcgctc cgcggggcgg acttcgtgat caccgtgttc
     8881 caggtgggag gcgtcgaggc ctacgcgcac gacgtcctga tccctcgtga gtacgggatc
     8941 gatcagacgg tgggggacac cttggggccg ggcgggatct tccgagggct ccgaacggtg
     9001 gaggccctcc gtgtggtggc cgaggccatg ctggagatct gccccgacgc ctggctgctg
     9061 aactacgcga acccgatgtc gatcaactgc tgggccacgg acctgctcgg cgtgaagatc
     9121 gtgggcctgt gccacagcgt gcaggggacc tccgagctgc tggcccgaga gctcggcgtg
     9181 ccgtacgacg aggtgacctt cgactgcgcc ggggtgaacc acacggcctg gttcaccacc
     9241 ttccgccgcg gtggcgagga cctgatcccg aggatccggg aggtcatgcg ggcccgccac
     9301 gtggaccggt cgatcccatt gctcccgcgc tcggacgatc cctacgagag catcgagcgg
     9361 gtccgggccg aactgatgat gctcaccggc tacttccaca cggagtccag ccatcacgcg
     9421 agcgagtact gggcctggtt ccgcaagacg ccggagctca ggcaggagta cctcgatcgg
     9481 cggtgggact acctggcgat ctgccaaagc gccgatgcct cctacacgaa cgaggatctg
     9541 gtcgaagagg ccgagcgcgg cgggctgcac cacggcggtg agttcgcggc cccgatcatg
     9601 gacagcatcg tgaccggcgc cactcgcgtg gtctacggga acgtccggaa cggagggtcg
     9661 atcgagaacc tggctcccga agcgtgcgtg gaggtggcct gcgcggtgga cgggaacggg
     9721 atccggccgc tccgctacgg gatgctgccc ccggcctgcg cggcgctgaa ccagctccag
     9781 atcgccgtcc aaggactgac cgtggaggcg gcgatgaccg gggatcgcga cctggctcac
     9841 gccgcggtgg cgatcgatcc cctcaccagc gcggtactcg cgctaccgag gatccacgag
     9901 atggtggacc ggatgctcga ggccgaggcg ccgtggctcc cacggttcga gccgcgggcg
     9961 accgcccggg cctgaacgat gcccggtccc gtgccggcgg ggtcctctcg acgcgagctg
    10021 gtggaccttc ccctccacgc ctatcggccg gtgcccagga tccgcctccc tcagcacccg
    10081 gtgcatcgag cgcggttccc ggccatcgac gcccacaacc acctcgggcg ctggttgtcc
    10141 gagtggctgg atcggccggg ggagtgggcc gtgcgcagcg ttccccacct gctggatctg
    10201 atggaccgat gcaacctgcg ggccatcgtg aacctcgacg ggcggtgggg cgaggagctg
    10261 gaggccaacc tggaccgcta cgaccgggcg catcccgggc gcttcgtcac cttctgccac
    10321 gtggactggg ccgcggcctc ggagcgggag ctcggacggg cggcggcagc gtcgctccgg
    10381 gcgagcgtgg ccgcgggggc caagggactg aaggtgtgga aggacctcgg cctccacgtc
    10441 cgcgacgccg ggggtgagct cgtcctgccg gacgatcggc gcctggcgcc gctgtgggag
    10501 accgcggccg agctccgcgt gcccgtgttc atccacacgg cggacccggt ggctttcttc
    10561 gacccggtgg acgagcggaa cgaacggctg gagcagctcc tcgcgcaccc cgagtggtcc
    10621 ttcgccgacc cttcgttccc gcggttcgag cggttgatcg aggcgttcga agggttggtc
    10681 tcggcccagc cggacaccac gttcgtgggg gtccacgtgg ctgggtacgc cgaggacctg
    10741 tcctgggtgg gccggatgct gggcgagcac ccgaacctcc atatcgacat cgcggctcgg
    10801 gtggccgagc tcgggcgcca gccccgcgcg gcacgtgagc tcgtcctggc ctaccccgac
    10861 cggatcctgt tcgggatcga cgagttccca cccgaccccg cgaactacgc gatctacctc
    10921 cgcttcctgg agacggccga tgagcatttc ccgcactccg ccgaggagat cccgctgatg
    10981 ggccggtggg ccatctccgg actgggtcta cccgatgaag tgctgcgcaa ggtctatgcg
    11041 gacaacgcgc tccgggtcgt tccggggctc gatacctcgg aaggagggac atcgtgaacg
    11101 gcggagcgag cacgcggccg cgaggcctca tcgcctggat cgggtgcatc ttcctcggcg
    11161 tctcgatgat ggtcccggcg gcaacggcgg gtgcggcgcc agggtcggag ggacccgtgc
    11221 gtctcaccgt cctgcacccc acgggtatcg tcgacgccac cggcacgcat tccggcaacg
    11281 tcggtgcgct cgcgaagagg gatcagtccg gcgcccggga cgacccgtcc cgatacgtct
    11341 ggctccgggg acgggggagg gcgtatgggg ggtaccgcaa ctacacgctt cccggagcgg
    11401 tgtcgccggc ggacgtgacg acgatcacgc tcaccgcgaa cttccgcggc cccatcgccc
    11461 gtgcgaacgc ctggacctgg gccgcgtacg atcgggagga gggtcgctgg gtgaggctcg
    11521 gtgatcagcg acactgcggc ggcgacgccg gcacggccca gtggccctgt gacgatctcc
    11581 agcgccgccc gtggaagtac gtgcggtaca atgtgatccc gccctcgggc gaggacctgt
    11641 ccgatttcat cgacccgtcg accggccaga tgcggatccg gctgcgcgca tcacgatcgg
    11701 ggcgggccag gctggactgg gaggcgctcg aggtctactc gaaccccggc cctgcagagg
    11761 cccgatggac gcccccggtg ggagcgcgtt ggcagtggca gctggagggc agggcgggac
    11821 atcacctgtc caccggcggc atcgccgtcg gcatctgcca accgccgttc ggtggagcta
    11881 cgtgcgtccg gccggacctg ttcgacatcg atctgtacgt ggacccggcg atcgccggac
    11941 ggttcggcta ccggctggag aggcccgccg tgaaggcgat ccacgcctcc gatcgccacg
    12001 tgatcgggta cgtgacggca ggggacatcg agcgctggcg tcccgactac gagcagttca
    12061 tcgacttcga ccggcgctgc gatggatgcc tgcggggcaa gccgttcagc aagaggttcc
    12121 ccgatgagta ctgggccaac ctgaacaagg gccaggggca gttcgccttc atgctgcaga
    12181 tgctgcgtgc tcgtaccgat cgggtggcgg ccacggggtt cgacggcatc gagtacgaca
    12241 tcgtcgatac ctacgaccag ggccggcaga ccacggggtt caagatcagc gcccagcggc
    12301 agctcgcgta caacagggcg ctggctcgga tggcgcatgc ggatgggctg tccgtggcgc
    12361 tgaagaacga cggggggcag atccaggagc tgctccactc cttcgacttc gccatcaacg
    12421 agcagtgctt ccagtacgac gagtgcgcgg gcggcggcta tccgtcaccc gggtggaggg
    12481 cgttcatcga ggccggtaag gccgtgttcc aggccgagta ccggctccag cctcgcgact
    12541 tctgccggaa ggcgaaccgg tgggacttca gctcgatcgt gaagtcccgc gacttcagcc
    12601 tctacgcgct gccgtggacg ccgtgccgct gagcaggtcg aggaggggtc gttagcggat
    12661 cgcggcgccg gtccgcaggt cgaacaggtg gatccgctcg gggcggatcc ccaggcgcac
    12721 ccgctccccg ggggtcatcc gcacgtccgg gtcgaagcgg accgtgatcg gcgcccgcga
    12781 gccctcgatc atcgtgggca cctcctcctc ctcggcgccg gtctccacca tcgtcgcccc
    12841 ggcgctgccg tggaccacca cctcgtcacc cagcggctcc accacgtcca ccaggaagtc
    12901 gaaggtgggc agtccgtcgg gggaggcgga gaccgcctcg ggccgcatgc cgaccgccac
    12961 atcgccgtcg ggaacgccgg gccgctcgaa cgtgaggtcg cccgccacga tcgtcccgct
    13021 ccgtgctcgc ccgcggatca ggttcatggg ggggctgccg atgaagccgg ccacgaacag
    13081 gttcgcaggg cggtcgtaga cctcctgggg gtccccgatc tgctgcagct tcccgttgga
    13141 catcaccgcg atccggtcgc cgagggtcat cgcctccacc tggtcgtggg tgacgtagat
    13201 tgtggtgacg cccaggcggg cgtggagccg cttcagctcg gcgcgcatct gcacccgcag
    13261 cttggcatcc aggttcgaca gcggctcatc caggaggaag gcgctcggct cgcggaccag
    13321 ggcccgtccc atcgccaccc gctggcgctg gccgcccgac agctgggccg ggcggcgctt
    13381 cagcaactcg tccagaccga gcatggcgct cacctcgcgc acccggcggt cgatctcggc
    13441 cttgcccacg tggcgctgcc tcagcccgaa ggcgaggttc cggtacacgg tcatgtgcgg
    13501 gtacagggcg tagttctgga acaccatcgc gatgtc
//
LOCUS       DASQQQ010000258         2557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_45222, whole genome shotgun sequence.
ACCESSION   DASQQQ010000258 DASQQQ010000000
VERSION     DASQQQ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45222"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VE646_09815"
     CDS             complement(<1..290)
                     /locus_tag="VE646_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HZD80320.1"
                     /translation="MKARVLSTGFHVPARVVTNAELTGYMDTSDEWIRQRSGIEERRW
                     VDEGVGGSDLARIAAEMALERAAMSASGLDCVIVATLSPDHFFPGTGVFLQR"
     gene            503..1489
                     /locus_tag="VE646_09820"
     CDS             503..1489
                     /locus_tag="VE646_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80321.1"
                     /translation="MAKTRRGAGTRRRKAGKRGDGPSPQQIRDAVERARGWLSERRDY
                     YGYLARQSSRRGAPEMGGHLRGDILARQDPDGSWGGDLAATAEAAWQLLDLGMSPDAP
                     ALRRALDWLYTRRDAGGAYAEGCTPARHEQGVCEHYLTGFFSPGAADEPQEIALPNGQ
                     SISSDASARLLASERALRTVLRIKPDDLRARDSVSGLRGVPLYLDYGGTFTPALLVGA
                     VQALTWSPGPPSGELTAGLDTLASHQVRDGTWPNVEFFFVLETLLEAPPARALPMFER
                     ALPYLLKAQYNNGAWGRRYQAAQTWIGLQVLERVAAARRAAKLAARSGATVR"
     gene            complement(1600..>2557)
                     /locus_tag="VE646_09825"
     CDS             complement(1600..>2557)
                     /locus_tag="VE646_09825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZD80322.1"
                     /translation="LEPPVAPGVGPEVDDLERVDDPASARRALRSIGQLQRSAVAGAL
                     EQLDVTDETAREIEEAFGILSEGQARLVEHWFAHEALREADLLAQVFHDLRSPLTSVL
                     FLSDALYSGQSGSLSDAQRRQVQLIYAASLTLLTLVDNVLSSRNLEAGEVEPERVPFS
                     LSALSGEVERITSPLAAQRKLELSFHMRAGTARIGDPDALRRVLLNLVTNAINYTDEG
                     GVTVRFLDAGDDLQAIVEDTGPGIDDEELAELFTPFRGRRARAAERPGRRRRFSGAGL
                     GLSICHRLTRLMDGDIWVDTEMGKGSRFVVQVPFPSVGHGIR"
BASE COUNT          385 a          879 c          939 g          354 t
ORIGIN      
        1 ttccgctgca ggaagacgcc cgtgcccggg aagaagtggt ccgggctcag cgtggccacg
       61 atcacgcaat ccagaccgga ggccgacatg gccgcccgct ccagcgccat ctccgcggcg
      121 atccgggcca ggtcggaccc gcccacgccc tcatcgaccc agcgccgctc ctcgatcccg
      181 gagcgctgcc ggatccactc gtccgacgtg tccatgtagc ccgtgagctc cgcgttcgtc
      241 acgacgcggg cgggaacgtg aaagccggtg gagagaacgc gcgctttcat gacgtttgaa
      301 tgtcgaatgt cgaacttcga acgacgaatg tcgagggccc ggaccggcgg tctccggtcg
      361 cagcctggcg actcgacggc cgctccacct gtaaggcagc tcaccaattg ctcgatcggc
      421 ccgccgcgta tcctcgcgcc cgacgctcgg atgtggtaat ctacggcaaa ctccggcccc
      481 tgacgaccgg gaggagacac cagtggccaa gaccaggcga ggcgcgggaa ccaggcgccg
      541 gaaggccggg aagcgggggg acggcccgtc gccacagcag atccgggacg cggtggaacg
      601 cgcccgaggc tggctgtcgg agcggcggga ctactacggc tacctggccc gccagagcag
      661 ccgccggggc gcccccgaga tgggtggcca tcttcggggc gacatcctgg cccgccagga
      721 cccggacggc agctggggcg gggacctggc ggccacggcg gaggccgcct ggcagcttct
      781 cgacctgggg atgagcccgg acgcgccggc cctccggcga gccctggact ggctgtatac
      841 gcgccgggac gccggggggg cgtacgccga aggctgcacg ccggcgcggc acgagcaggg
      901 ggtctgcgag cattacctga cgggcttctt cagcccgggc gcggcggacg agccccagga
      961 gatcgcgctc ccgaacggcc agtcgatctc gtccgatgcc agcgcgcggc tgctggcctc
     1021 cgagcgcgcg ctccgcacgg tcctgcggat caagccggac gacctgaggg cccgggactc
     1081 ggtgagcggt ctgcgggggg ttccgctgta tctggattac ggcgggacgt tcacgccggc
     1141 gctgctggtc ggagcggtcc aggccctgac ctggtcgccg ggtccgccgt cgggcgagct
     1201 gacggccggc ctggacacgc tggcctctca ccaggtgagg gacggcacct ggcccaacgt
     1261 ggagttcttc ttcgtcctgg agaccctgct ggaggcgccg ccggcccgcg ccctgccgat
     1321 gttcgagcgg gccctgccct acctgttgaa ggctcagtac aacaacggcg cctggggccg
     1381 ccgctatcag gcggcgcaga cgtggatcgg cctgcaggtc ctggagcgtg tggccgcggc
     1441 ccggagggcg gcgaaactgg cggcaagatc gggagcgacg gtccggtgat cggtattcgg
     1501 tattcggtga tcggtattcg gtgatcggta ttcagtactc ggccctcggt gctcggtcga
     1561 ggcgccgggg ccgaccgaat accgaggacc gaacaccgat taccgaatcc cgtgacctac
     1621 cgatgggaac gggacctgga ccacgaagcg cgaccccttg cccatctcgg tgtccaccca
     1681 gatgtcgccg tccatgagcc gggtgagccg gtggcagatc gaaaggccca ggcccgcgcc
     1741 ggagaaccgg cgtcggcgac cggggcgttc ggcggcgcgc gcgcgacggc cgcggaacgg
     1801 cgtgaagagc tcggccagtt cctcgtcatc gatccccgga ccggtgtcct cgacgatcgc
     1861 ctggaggtcg tccccggcgt ccaggaacct caccgtcacc ccgccctcgt cggtgtagtt
     1921 gatggcgttg gtcaccaggt tcaggagcac gcgacgcagg gcgtcggggt cgccgatccg
     1981 cgccgtgccg gcccgcatgt ggaagctgag ctccagcttc cgctgcgcgg ccagcgggct
     2041 ggtgatgcgc tccacctcgc cggacagggc ggagagggag aacggcaccc gctccggctc
     2101 cacctcgccc gcctccaggt tcctggagct gaggacgttg tccaccaggg tgaggagcgt
     2161 gagggaagcg gcgtagatga gctggacctg ccggcgctgc gcatcgctca gcgagcccga
     2221 ctgcccggag tagagggcgt cggagaggaa gagcacggag gtgagcgggg atcgcaggtc
     2281 gtggaagacc tgggccagga ggtccgcctc gcggagggcc tcgtgggcga accagtgctc
     2341 gaccagccgg gcctggccct cgctcaggat cccgaacgcc tcctcgatct cccgggccgt
     2401 ctcgtcggtc acgtccagct gctccagggc gccggccacc gcggagcgct ggagctgtcc
     2461 gatggaccgg agcgcccggc gcgccgacgc cggatcgtcc accctttcca agtcgtccac
     2521 ctcggggccg acgccggggg cgacgggggg ctcgagg
//
LOCUS       DASQQQ010000259        12158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_265798, whole genome shotgun sequence.
ACCESSION   DASQQQ010000259 DASQQQ010000000
VERSION     DASQQQ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12158
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265798"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VE646_09830"
     CDS             <1..649
                     /locus_tag="VE646_09830"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HZD80323.1"
                     /translation="SLVFLQPDLGTSIVLAAILVGVLVVGGARARHLAILALTACVLM
                     FGAFQLGVVKDYQVARLQSWLDPTNDPQRSDYNKQQAEIAIGSGGMLGRGYLHGTQTN
                     LDFVPEQHTDFIFTVVGEEFGFAGAMSVLVLFALLIWRAYRIAVLSKDPFGTYVAAGI
                     ASMFAIQVFVNVGMTLGIMPITGIPLPFVSYGGSSLLTNCLAVGLLLNIHMRRLA"
     gene            complement(654..1829)
                     /locus_tag="VE646_09835"
     CDS             complement(654..1829)
                     /locus_tag="VE646_09835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZD80324.1"
                     /translation="MEPGILDHAPSVPAGPNERVRVMVVDDNAGFRESLLVLLESGEL
                     EVVAEADSGERALELVGDMEPDVVLMDVRMPGIDGIETTRLLKQRFPGIGVVALSGHE
                     DQDIVRGMLVAGASGYVLKDSDGDDILDAVMQAAVGGGVLSPMVTPRVIEDLTEALER
                     ERRRWKELEEAHVALVERSARRHELVARLSHELRTPVTVILGMAQTLAKGAGSPEQQH
                     ELLDRMIQRTQDLAWLVERFEFTMDAGMTEQVHVADIAAEVAERDARITVTAPEGLPP
                     ASLNPVVARRVLAELVDNALRFSPEPAPVAVRVDTRQGHIEVRVVDRGSGIPIADRER
                     IFEVLEQLEDLNVRVHQGAGIGLSLARTAARAMGGDVAVEDSGPGGSTFLWTIALDD"
     gene            2836..4491
                     /locus_tag="VE646_09840"
     CDS             2836..4491
                     /locus_tag="VE646_09840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HZD80325.1"
                     /translation="MGTTDGDGSGARRPRRRRRGTTATEEDVGRPEPAAKATGTKRRT
                     EPEPEPETPEEKPKPARGRRRSKAGSTAETETAGKVDGTAKAEPQPAPSRGRRRGRAS
                     TTGTATAGPTRLRRSRQTSGRRRRAEVMPIVPRVTDKLMVITEHGERDQIAILEERNL
                     VQHYVTRAGARSMVGNVYLGRVQNVLPGMEAAFVDVGRGRNAVLYAGEVNYSPEDLES
                     GSPPRIEQVLKSGQSVVVQVTKDPIGGKGARLTAQISMPGRYLVLVPDSNVTGISRRV
                     PDEERKRLKQILKRIRPEGHGIIVRTAAEGADGEALKSDVERLQQEWSAIQKRAKKAK
                     APALLYEEPELTVRAVRDLFTNEEFRELVTDSERVYALVTSYLEQVAPDLRQKVQMHR
                     GKLPVFEEFHVVEQIHKGLDRKVWLPSGGYLVIDRTEALTVIDVNTGKSVGKHNLEET
                     VVNTNVEGAREVARQLRLRDIGGMIIIDFIDMLLEQNKRKVVDTLREELARDKTRSQV
                     FDITPLGLLEVTRKRVSGGLLEAFSETCPTCEGRGVILTYDVK"
     gene            4504..4884
                     /gene="rplU"
                     /locus_tag="VE646_09845"
     CDS             4504..4884
                     /gene="rplU"
                     /locus_tag="VE646_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HZD80326.1"
                     /translation="MYAVIKAGGKQHKVKAGDVIEVEYMHGADQTVTFHPLLVVDDEG
                     KTHVGRDAQQAVVTAKLVGEQKGDKVKIFKYKNKTGYARKAGHRQLMTLLEISEVSLS
                     TRKPAAKKEAATAVAQEPAEEGVE"
     gene            4922..5176
                     /gene="rpmA"
                     /locus_tag="VE646_09850"
     CDS             4922..5176
                     /gene="rpmA"
                     /locus_tag="VE646_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HZD80327.1"
                     /translation="MAHKKGGGSSKNGRDSESKRLGVKAFGGQQVTAGSIIVRQRGTR
                     IHPGEGVGKGGDDTLFATVDGVVGFHTSRGRCVASIAPRS"
     gene            complement(5180..5608)
                     /locus_tag="VE646_09855"
     CDS             complement(5180..5608)
                     /locus_tag="VE646_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80328.1"
                     /translation="MPEPITVWAVELGRSPNDRKGTLTLREDGLHFEPGDGSREISIA
                     LAELRKVRRLRGSPVLMVAHLEEGRIRRTAFYFVQPPPLEPSRDQPPERVPNLFSSMG
                     KHSRRRIRRDNAGYLGLWNREKKRAVAEWADAVRAAAETA"
     gene            5654..6904
                     /gene="obgE"
                     /locus_tag="VE646_09860"
     CDS             5654..6904
                     /gene="obgE"
                     /locus_tag="VE646_09860"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HZD80329.1"
                     /translation="MTFVDETVVFVRAGRGGDGSNALLREPFKPRGGPDGGNGGDGGD
                     VVLEVSAGVRDLSWLAEHPHRRAESGRAGGRNRRDGARGRDLVATVPDGTTVFDEDGL
                     LADLVGEGARAVVGRGGRGGRGNVALASGRNRVPRAAEAGEPGEERRLRLELRVVADV
                     GLVGLPNAGKSTLLSSLTAAKPKIAAYPFTTLTPNLGVAEGEQRFVLADIPGLVEGAH
                     EGRGLGDRFLRHVSRCRALVLVVDLSAPDPAADLATVRTELEAYDPALASRPSLVAGT
                     KADLVTDAAASAAALHRGALAVSGISGQGIGSLADRLAGLSTRAAEVAEERRPYVVLR
                     PARPSFVVKREGERFRVVGRNVERWVADTDFDDPRRVVALQKRLLKAGVERELAATGA
                     HPGDEVVIGDRTFEFLPGGEGEVE"
     gene            6904..7350
                     /locus_tag="VE646_09865"
     CDS             6904..7350
                     /locus_tag="VE646_09865"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HZD80330.1"
                     /translation="MEPRDALVREEEEGWAELRAQVDLLDAAQLQEPGLNEDGWSVND
                     LLWHLGCWWAEAATQLERVRLGTYEEHDVPEDDMNARFLEEGRRQDPSEVLALLLAAR
                     SRARQELAALDELAPAAHEWFEESGSLHYREHLGDLRRWVSRVGGS"
     gene            7440..8015
                     /gene="nadD"
                     /locus_tag="VE646_09870"
     CDS             7440..8015
                     /gene="nadD"
                     /locus_tag="VE646_09870"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HZD80331.1"
                     /translation="MGGTFDPVHNGHLLTAEEAAVQFGLQEVVFVPTGQPWMKGERDV
                     TPPEHRYLMTVIATASNPRFSVSRIEVDREGPTYTVDTLRALREQAGDRADLFFVTGA
                     DAMLEILQWKDPEEVLSLAHFIAATRPGYDITHFEAQAAAAHPNITVMNIPALAISST
                     DIRERVRAGRPIRYLVPDGVQSYIDKAGLYR"
     gene            8012..9019
                     /locus_tag="VE646_09875"
     CDS             8012..9019
                     /locus_tag="VE646_09875"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HZD80332.1"
                     /translation="MRGNHRTYRRVPRAAVWAGTGLIAAALLASGLRWRSEVRSAVAT
                     GRPSTPPERALLLVDTRAGPLLAVVATGGSAGPAVMTVPAAVSLTIPGQGDGRVKGLG
                     RLPGPEVALAISNLLGVWIPHEAITDAAHLAAVVDRARGVQAFGQARSGAEIRRMLGR
                     PGSGAELEWQETLRGLFEVAAAWRSDDFLGSDDPGAVAVLLQSAKGASVVGMPTNEAA
                     AGFLLPDPDTLTHVVAARLGGPPEAPIPVIVLNGSGTPGVGRSVAERLVPGGFRVTVS
                     QNASSFDHDTTLVLAGSSADRTAADRVRELLGVGTVSVAGVPSSLGDVTIVVGRDYQT
                     G"
     gene            9038..9427
                     /gene="rsfS"
                     /locus_tag="VE646_09880"
     CDS             9038..9427
                     /gene="rsfS"
                     /locus_tag="VE646_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HZD80333.1"
                     /translation="MPGRQAGLAHSRDVAVAAARTASDRQAEDVVILDVRELIVITDY
                     FVIAGGTSERQVRTLVEEIEKALKRIGVRPVRREGESGARWVLLDYVDVVVHVFAEEE
                     RAYYDLERLWRDAARVPWEDSDAASSG"
     gene            complement(9441..9605)
                     /locus_tag="VE646_09885"
     CDS             complement(9441..9605)
                     /locus_tag="VE646_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80334.1"
                     /translation="MTWVWAVAGWFLVSFALGPVIGRWLKHMAPRRSRPAATHLPRPE
                     YAPLAARVAS"
     gene            9850..9922
                     /locus_tag="VE646_09890"
     tRNA            9850..9922
                     /locus_tag="VE646_09890"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9883..9885,aa:Ala,seq:ggc)
     gene            10080..10313
                     /locus_tag="VE646_09895"
     CDS             10080..10313
                     /locus_tag="VE646_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80335.1"
                     /translation="MAVAGELDRRPKHVLPAFQVALGGLELALCRLLGDADPVLLILQ
                     EIERDGVRVERLQQLAPFLGEPGESLRQDDPAL"
     gene            complement(11614..>12158)
                     /locus_tag="VE646_09900"
     CDS             complement(11614..>12158)
                     /locus_tag="VE646_09900"
                     /inference="COORDINATES: protein motif:HMM:NF025186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HZD80336.1"
                     /translation="RQEIDFKDFTFKKARIFDVVGSPRLRHRVECNGFFARLTRACRG
                     RGWELEWIGERRAARRWDGVINPDGIGRIRSWDRQISFFLEYDRGTENHDQLANKTYH
                     YRIVGRTVTSEDALLFVFPSAARERYARSALIGASVPVATTVRADVLRHPLGPVWLPL
                     DDDRRRTILDLPRLPRDDSG"
BASE COUNT         1820 a         4143 c         4443 g         1752 t
ORIGIN      
        1 cagcctggtg ttcctgcagc cggacctggg cacctccatc gtgctcgcgg cgatcctggt
       61 tggcgtcctg gtggtgggcg gggcccgggc gcggcatctc gccatcctgg ccctcacggc
      121 gtgcgtgctg atgttcgggg cgttccagct gggcgtggtg aaggactacc aggtggcccg
      181 gctccagtcc tggctggatc ccacgaacga cccccagcgc agcgactaca acaagcagca
      241 ggccgagatc gcgatcggct ccggcggcat gctcggccgt ggctacctgc acgggaccca
      301 gacgaacctc gacttcgtgc ccgagcagca cacggacttc atcttcaccg tcgtgggcga
      361 ggagttcggg ttcgccggcg caatgtcggt gctggtgctc ttcgccctgc tgatctggcg
      421 cgcctaccgg atcgccgtgc tgtcgaagga cccgttcggg acctacgtgg ccgccggtat
      481 cgcgtcgatg ttcgcgatcc aggtcttcgt gaacgtgggg atgaccctcg gcatcatgcc
      541 gatcaccggg atccccttac cgttcgtgtc ctacggaggg agctccctgc tcacgaactg
      601 cctggcggtg ggcctgctgt tgaacatcca catgcgccgg ctggcctagc ggctcagtcg
      661 tccagggcga tcgtccacag gaaggtcgag ccgcccggcc ctgagtcctc caccgccacg
      721 tcgccaccca tggccctggc tgcggtgcgg gccagggaca ggccgatccc ggcgccctga
      781 tgcacgcgga cgttgagatc ctcgagctgc tcgagcacct cgaagatgcg ctcccggtcc
      841 gcgatgggga tgccggagcc gcggtccacc acgcggacct cgatgtgccc ttgccgggtg
      901 tcgaccctca cggctacggg cgccggctcc ggggagaagc gcagggcgtt gtcgacgagc
      961 tcggccagca cgcgccgggc caccaccggg ttcaggctgg cgggaggcag gccctcgggt
     1021 gccgtcaccg tgatccgggc atcccgctcg gccacctcgg cggcgatgtc ggccacatgc
     1081 acctgctcgg tcatcccggc gtccatggtg aactcgaacc gctccaccaa ccacgccagg
     1141 tcctgcgtgc gttggatcat gcggtccaac agctcgtgct gctgttcggg gctccccgcc
     1201 cccttggcca gcgtctgcgc catgccgagg atcacggtga ccggggtccg cagctcatgc
     1261 gacagccgag ccacgagctc gtggcgacgc gccgagcgct ccaccagggc cacgtgggcc
     1321 tcctccagct ccttccatcg gcgccgctcg cgctccagcg cctcggtcag gtcctcgatc
     1381 acccgagggg tgaccatggg cgacaggacc cctccgccca ccgccgcttg catcaccgcg
     1441 tcgaggatgt cgtccccatc cgaatccttc agaacgtagc cggaggcgcc ggccaccagc
     1501 atgccccgaa cgatgtcctg gtcctcgtgg cctgacaggg ccaccacacc gatccccggg
     1561 aaccgctgct tgaggagtcg agtggtctcg atcccgtcga tgcccggcat ccgcacgtcc
     1621 atgaggacga cgtcgggctc catgtcgccg accagctcga gcgcacgctc gcccgaatcc
     1681 gcttcggcca cgacctccag ctcgcccgat tccaggagca cgagcagaga ctcgcggaag
     1741 ccggcgttgt cgtccaccac catcacgcgg acccgctcgt tgggccccgc gggaacggag
     1801 ggagcgtgat ccaagatccc cggctccatg gatgcaagtg tcccgggcgg gccctcgagc
     1861 gtgtatcggg aggaagccgg gtttgccggc ccggacgcgg gccacgcccg actagcacct
     1921 tcggtctagc ccggacaagt ggagcgcggc accccgcgcc tcccggtgcc catccccggt
     1981 gcttgctatg ctcggaggtg agcttgctcg ccggcccggg gccggacgcc ccgaaggaca
     2041 ccggcgagga cgagtggcgc ggtggggcca cgccggcccc gggacggagg gtcccggctg
     2101 gccgcggccc agcatcggcg ccgggaacag ggcgtacttc gtgcgaagcg caagggcgcg
     2161 gagcgccggg gcggatcctg ccgccggcgt ggaccgcgcg catcggtcga gcggatcccg
     2221 cggcatcgcg accgctcctt ctgcccagca gcgcgcccaa gtcgtcgccc gcccgtcgcc
     2281 gccgcgcccc atgggagttg ggaaggagtt ggacggatga cgatggacga ggcggagccg
     2341 accgcgacga cgaccgaacg gaaacggacc gggtcggacg gcacgggagg tccagggggc
     2401 accacccctc gaccccgccg gcggcggggc agccggggac ggggacgagg gcgccggcgc
     2461 ccgagcggcc agcaacccac caccgggaca ccggaggctg aggcctcccg gccctcggag
     2521 gaggcgagcc caggtccggc cgctggaggc gaacaggagg ccccgcccgc cccacgcaag
     2581 cgcggcggca gcggcggccg gcggcaagac cggacggggt cctcgggccg gcagaccgct
     2641 gagcgcgcta cggaggcgga ggccccggag ggcggagcgg gtgaggcgcc cgcccccagg
     2701 cgtcgccgcc gtcgcggcgg gcgcggccgg gggggacgcg cccggcgtcc caccgaacag
     2761 gccagccaga cgaccgagga gggcgggacg gcgcaggccc aggccgagcc cgaacccgcc
     2821 gaaccccggg ggccgatggg aaccaccgac ggagatggat ccggcgcccg tcggccgcgc
     2881 cggcggcgtc ggggcaccac cgccaccgag gaggatgtcg gacgtccgga gccggcggcc
     2941 aaggccaccg ggaccaagcg gcgcacggag cccgagcccg aacccgagac cccggaggag
     3001 aagcccaagc ccgcgagggg gcgtcggcgg agcaaggcgg gatccaccgc ggaaaccgag
     3061 acggcgggca aggtggatgg aacggcgaag gccgagcccc agcccgctcc ctcccgcggc
     3121 cgacgccgcg gtcgggcctc caccacgggc accgcaaccg ccggacccac ccggctgcgg
     3181 cgctcgcgcc agacctccgg acgtcgtcgg cgggccgagg tgatgccgat cgtccccagg
     3241 gtcaccgaca agctgatggt catcaccgag cacggcgagc gggatcagat cgcgatcctg
     3301 gaggaacgga acctcgtgca gcactacgtg acccgtgcgg gggcccggtc catggtgggc
     3361 aacgtgtacc tgggtagggt gcagaacgtc ctgcccggga tggaggcggc gttcgtcgac
     3421 gtgggacgcg gccgcaacgc cgtcctgtac gccggcgagg tcaactactc gcccgaggat
     3481 ctggagagcg gctcgccgcc gcgcatcgag caggtcctga agagcggcca gtcggtggtg
     3541 gtccaggtca cgaaggaccc gatcgggggc aagggggcgc ggctcacggc acagatcagc
     3601 atgccgggcc ggtacctcgt gctggtgccc gactccaacg tcaccgggat cagccggcgg
     3661 gtacccgacg aggagcgcaa gcgtctgaag cagatcctca agcgcatccg cccggagggt
     3721 cacggcatca tcgtccgcac ggcggcggag ggcgcggacg gggaggcgtt gaagtcggac
     3781 gtggagcggt tgcagcagga atggtccgcc atccagaaga gggcgaagaa ggccaaggca
     3841 ccggccctcc tctacgagga gcccgagctc accgtccggg cggtccgtga cctgttcacg
     3901 aacgaggagt tccgcgagct ggtgacggac tccgagcgcg tgtacgcgct cgtgacctcc
     3961 tacctcgagc aggtcgcccc ggatctgcgg cagaaggtgc agatgcaccg cggcaagctc
     4021 ccggtgttcg aggagttcca cgtcgtcgag cagatccaca aggggttgga ccggaaggtc
     4081 tggctcccct cgggaggcta cctggtgatc gaccggaccg aggccctgac cgtgatcgac
     4141 gtgaacacgg gcaagtcggt cgggaagcac aacctcgaag agacggtggt caacacgaac
     4201 gtggagggcg ccagggaggt ggcgcggcag ctgcggctcc gcgacatcgg cgggatgatc
     4261 atcatcgact tcatcgacat gctgctcgag cagaacaagc ggaaggtcgt cgatacgctc
     4321 cgcgaggagc tggcccggga taagacccgg tcgcaggtgt tcgacatcac ccccctcggg
     4381 ctgctggagg tgacgcgtaa gcgcgtgtct ggtggactct tggaggcgtt ctccgagacc
     4441 tgtcccacct gcgagggtcg gggcgtgatc ctgacctacg acgtgaagtg agaggagcgg
     4501 acgatgtacg cggtcatcaa ggccggtggc aagcagcaca aggtgaaggc cggtgacgtg
     4561 atcgaggtcg agtacatgca cggggccgac cagaccgtga cgttccaccc gctgctggtg
     4621 gtagacgacg aggggaagac gcacgtgggc cgggacgccc agcaggccgt ggtgacggcc
     4681 aagctcgtcg gcgagcagaa gggcgacaag gtcaagatct tcaagtacaa gaacaagacc
     4741 ggctacgcgc gcaaggccgg ccatcggcag ctgatgacgc tgctcgagat ctcggaggtc
     4801 tcgctctcga cccggaagcc ggcggcgaag aaggaggccg ccacggcggt cgctcaggag
     4861 ccggcggagg agggcgtcga gtagcctcac tcggccggtg ccgtttggta ccctcgatcc
     4921 catggcgcac aagaagggcg gcggctcgtc gaagaacggg cgcgattccg agtccaagag
     4981 gctcggggtg aaggcgttcg gcggtcagca ggtgaccgcc ggctccatca tcgtgcgtca
     5041 gcggggcacc cgaatccatc cgggcgaggg cgtgggaaaa gggggcgacg acacgctgtt
     5101 cgccacggtg gacggggtgg tgggcttcca cacctcgcgg ggccgctgcg tggcctccat
     5161 cgctccccgg agctgagcct caggccgtct cggcggcggc acgtacggcg tccgcccact
     5221 cggccaccgc gcgcttcttc tcacggttcc acagcccgag gtagccggcg ttgtcccgtc
     5281 ggatccggcg cctgctgtgc ttgcccatgc tggagaagag gttggggacg cgctcgggcg
     5341 gctggtccct ggaaggctcc aaggggggag gttggacgaa gtagaaggcg gtgcgtctga
     5401 tccgaccctc ctcgagatgg gcgaccatga gcaccggcga tccgcgcaga cgccggacct
     5461 tccgcagctc ggccagcgcg atcgagatct cccgagaccc atcgccgggc tcgaagtgca
     5521 ggccatcctc cctcagggtg agggtcccct tgcggtcgtt cgggctcctc ccgagctcca
     5581 cggcccagac ggtgatcggt tcgggcatat cgccatcttc gccgagccct cggctttcga
     5641 ccacaatcgt gccgtgacct tcgtcgacga gaccgtggtg ttcgtccgag ccggcagggg
     5701 tggtgacggc tccaacgcct tgctgcgcga gccgttcaag cctcgcggcg gtcccgacgg
     5761 aggcaacggc ggcgacggtg gtgacgtcgt cttggaggtg tcggccgggg ttcgcgacct
     5821 ctcgtggctg gccgagcacc ctcatcggcg ggcggagtcc ggccgagcgg gcgggcgcaa
     5881 ccgccgcgac ggggctcggg gccgtgacct ggtggcgacg gtgcccgacg gaacgacggt
     5941 cttcgacgag gacggcctcc tcgccgacct ggtgggcgag ggcgctcggg ctgtggtggg
     6001 ccggggtggg cgcggcggtc ggggcaacgt ggccctggcg tcggggcgga accgggttcc
     6061 tcgggcggcc gaagccggag agcccggtga ggaacggcgg ctgcggctgg agctccgcgt
     6121 ggtggcggac gtcggtctgg tggggctccc caacgccggc aagtccaccc tgctgtcgag
     6181 tctcaccgcc gcgaagccca agatcgccgc ctatccgttc accaccctca ccccgaacct
     6241 cggcgtggcc gaaggggagc agcggttcgt gctggcggat atccccggcc tggtggaggg
     6301 cgcccacgag ggccggggat tgggcgaccg gttcctccgt cacgtgagcc ggtgccgagc
     6361 cctggtgctg gtggtcgacc tgtccgcccc agatccggcg gcggacctgg ccacggtgcg
     6421 cacggagctc gaggcctacg atccggccct ggcctcccgc ccctcgctgg tggccggcac
     6481 gaaggccgat ctggtgaccg atgccgccgc atcggcggcg gcgctgcacc ggggggccct
     6541 ggcggtctcc gggatctcgg gccaagggat cgggtcgctg gcggaccgtc tggccggtct
     6601 gtccacccgg gccgccgagg tggcggagga acggcgaccg tacgtggtcc tgcgtccggc
     6661 ccgtccctcc ttcgtcgtca agcgggaagg cgagcggttc cgggtggtgg gtcgcaacgt
     6721 agagcggtgg gtggcggaca cggatttcga cgatcctcgg cgggtggtgg cgctccagaa
     6781 gcggttgctc aaggccgggg tggaacggga gttggcagcg accggagccc atccgggtga
     6841 cgaggtcgtg atcggcgacc ggacgttcga gttcctgccc ggaggcgaag gggaggtcga
     6901 gtgatggagc cgcgggatgc gctggtgcgc gaggaagaag agggctgggc ggagctgcgc
     6961 gcccaggtgg acctcctgga cgcggcgcag ctgcaggaac cgggcctcaa cgaggacgga
     7021 tggtccgtga acgatctgct ctggcatctg gggtgctggt gggcggaggc ggccacccag
     7081 ctcgagcggg tccgtctcgg gacctacgag gagcacgatg tcccggagga cgacatgaac
     7141 gcccggttcc tggaggaagg caggcgccag gatccctccg aggtgctggc cctgctcctc
     7201 gcggccaggt cacgtgcccg gcaggagctc gccgctctgg acgagctcgc gccggcggct
     7261 cacgagtggt tcgaggagtc cggatcgctc cactatcggg agcatctcgg ggacctccgc
     7321 cgctgggtat cgcgggtggg gggatcctga gcgcggtctt ccggcgccgc aacggcagat
     7381 cccggaggcc gcgcccagct gggccgtacg atacgcgcgt gacacgtcgc ctgggggtca
     7441 tgggggggac cttcgatccc gtccacaacg gacacctcct gaccgccgaa gaggccgcgg
     7501 tccagttcgg cctgcaggag gtggtgttcg tccccaccgg gcagccctgg atgaaggggg
     7561 aacgggacgt cacgcccccc gagcatcggt acctgatgac ggtcatcgcc accgccagca
     7621 acccgcgctt ctcggtctcg cggatcgagg tcgaccgtga gggtcctacc tacacggtgg
     7681 acacgctccg cgccctgcgt gagcaggccg gcgaccgggc cgatctcttc ttcgtgacgg
     7741 gcgcggacgc catgctggag atcctgcagt ggaaggaccc cgaggaagtg ctgtccctcg
     7801 cgcacttcat cgcggccacc cgtcccggat acgacatcac gcatttcgag gcgcaggcgg
     7861 ccgccgccca ccccaacatc accgtgatga acatcccggc tctggcgatc tcctccacgg
     7921 atatccgcga gcgcgtccgt gccggacgcc cgatccggta cctggtcccg gacggcgtcc
     7981 agagctacat cgacaaggcg ggactgtacc gatgaggggg aaccaccgga cctaccgacg
     8041 cgtcccacga gccgcggtct gggcggggac cgggctgatc gccgccgcgc tgctcgcgtc
     8101 tgggttgcgc tggcgctccg aggttcgatc ggccgtcgcc accggtcggc cttccacgcc
     8161 cccggagcgc gccctgctcc tggtggatac ccgggctggt cccctgttgg cggtggtggc
     8221 tacggggggg agcgccggac ctgcggtcat gacggtgccg gccgcggtga gcctgaccat
     8281 ccccggccag ggcgatggac gggtgaaggg tctgggccgt ctgcccggcc cggaggtggc
     8341 gctcgcgatc tcgaacctgt tgggggtatg gatcccgcac gaggcgatca cggacgctgc
     8401 gcacctcgcg gccgtcgtgg accgcgcccg tggcgtccag gcgttcgggc aggcgcgatc
     8461 gggggcagag atccgtcgga tgctcggcag gccaggctcc ggcgcggagc tcgagtggca
     8521 agagacgctg agggggctgt tcgaggtggc ggcggcctgg aggtcggatg acttcctggg
     8581 atcggacgac cccggggcgg tggccgtgct cctccagagc gcgaagggcg catcggtggt
     8641 cggtatgccc acgaacgagg ccgcggccgg gttcctgttg ccggatcccg acacgctcac
     8701 gcacgtcgtg gcggcccggc tcgggggtcc cccggaggcc ccgatcccgg tgatcgtgct
     8761 gaacggcagc ggcacgccgg gggtgggccg atccgtggcg gagcgcctgg tacccggcgg
     8821 gttccgggtg accgtctcgc agaacgcctc cagcttcgac cacgacacca cgctggtcct
     8881 ggccggctcg agtgccgatc ggaccgccgc cgaccgcgtg cgcgagctgc tcggcgtggg
     8941 taccgtgtcg gtcgcggggg tgccatcctc gctcggggat gtcaccatcg tggtgggaag
     9001 ggactaccag acggggtgat gatgcacgta ggacgccgtg cccggtaggc aggccgggct
     9061 ggcccacagc agggacgtgg ccgtggcggc ggcgcggacc gcctccgacc gtcaggccga
     9121 ggacgtggtg atcctcgacg tccgcgagct catcgtcatc accgactact tcgtgatcgc
     9181 gggcggcacc agcgaacgcc aggtgcgcac cctggtcgag gagatcgaga aggcgctgaa
     9241 gaggatcggc gtccgtcccg tgcgccggga gggcgagtcc ggtgcccggt gggtgctcct
     9301 cgactacgtg gacgtcgtgg tgcacgtctt cgcggaggag gaacgtgcgt actacgacct
     9361 ggagcggctg tggcgggacg ctgcccgcgt gccctgggag gactcggacg cggcgtcctc
     9421 cgggtaggct cggtcgccgg tcaggaggcg acgcgagcgg cgaggggggc gtactccggg
     9481 cggggcagat gggtcgccgc cggtcgtgac cgcctgggag ccatgtgctt cagccaccgg
     9541 ccgatcaccg ggccgagcgc gaacgacacc aggaaccaac cggccaccgc ccacacccac
     9601 gtcatcgcca tctcccctcg acctccgact gagggaacca tcggcacctt cccatcccac
     9661 ttgaacgaag gccgggccac ggcgagaacc ctcggtgaac gatcggccac gggaggggtg
     9721 tcggttccgg gctctcgata tgaggcgtcc tcggccggcg agcacctgag gactgcgggg
     9781 ggcccggggc tcccgggtac aatctgcgtg catccggagg gggtgcccag gtcgggaccc
     9841 cgcccgtcag ggcccatagc tcagctggta gagcgcttgg ctggcagcca agaggtcagg
     9901 ggttcgagtc cccttgggtc cactttcagt ctcttattcg aactcgggcg agagaagaga
     9961 ggctcgaaga cctccgagaa ctccgcccgg tcgatccgtc ggtctttgat gtacacggcc
    10021 tggagcacgg cgtcgttgaa gcgacggcgt accgacggcc gagccttcaa gtatgccgcg
    10081 tggcagttgc cggcgagctt gatcgccgtc cgaagcacgt cctgccagcc ttccaggtcg
    10141 ccctcggcgg cctcgagctc gctctttgcc gcctgctcgg cgacgccgat ccggtcctgc
    10201 tcattcttca ggagatcgag cgggatggcg ttcgcgtaga acgcctgcag cagcttgccc
    10261 cgttcctcgg cgagcctggc gagagtcttc gtcaggacga cccggcgctc tgaggcctcc
    10321 gcctggcgct cgacgatctc ggcctccaat tcctcggtta gccgcttgac ccacaaggcc
    10381 ggcagctgga tccgtcggta caggtcctcg acgagtgtct cggcgtcggc ggccaggacg
    10441 tacggctggc ggcacccggt ccgggccgtc ccgcgctgtc ccaggcagta gaagtacagg
    10501 tacgtgccct tggcgagcgt gagggacagg cgcctgccgc actctccgca gtgaagcagc
    10561 cccttcaggt agtgctcgtg gcggcgctgg cgcaccccgg cgacgtcgcg cgagcgaagc
    10621 gtctgctgga cccgctcgaa cagggcctgg gagatgagcg ccttgtgctg gcctgggtag
    10681 cggacgcctc cccaccgcac cacgcccgtg tagaacgggt tcgcgagcag ctcggcgagc
    10741 ttggagaccg acaccggcgc gccgcgtttc cgggcgtacg gggaggtgag ccccttcgcg
    10801 tgcatcgtgg cctgcagctc gggaagtgag tactccccgg tcgcgtacag ccggaacgcc
    10861 tcccgcacga gcagggcccg ctcggggtcc agaaccacct tggcgatctc cttgccgggg
    10921 atcttctccc ggacgttcag gtacccgatc ggggccttgg tcggccagcc gcccatcttg
    10981 gctttctggg tcatgccctt tcggatctca ccggccaggt tcgccgagta gaactccgcc
    11041 atgagggcgt ggataccctc cacgaggcgt ccagaggccg tctcctcgat gttctcgctt
    11101 accgagacca gctggacccc ggcgcgccga agcgccgccc tgatcgcgac gtggtcctcc
    11161 atcgagcggg ccaggcgatc gatcttgtgg acgaccacgg cgccgatccc gccctctgcc
    11221 acccggcgaa ggagcgcctt cagcatgggc cggtcggccg tccgggcgga ctcgccggcg
    11281 tcggtgaact cacccgccag gttccagccg cgctcggcga tgttgcgaag gcacgcctcc
    11341 cgctgggccg ggatcgagta gccctcgccg gactcgtcct tggcggcctg ctccttggtg
    11401 gagacccgga ggtagatcac cgcgtccatc gctcctcctc ctcgtcctcg tgctccaggg
    11461 cgagcgccag atcgagcagc gcccgggcca gcgggcggag cgacctgggc tccggcacca
    11521 tcggtgcttg tcttaaacga cggagcctat ccgcggccgc tgtcgcgccc gaggcccggg
    11581 gaaggtcgct gcgagccgtc cgcccgcccg agctcacccg gaatcgtcac ggggcagccg
    11641 ggggaggtcc aggatcgttc ggcgccggtc gtcgtcaagg ggcagccaca ccggcccgag
    11701 ggggtggcgc aggacgtctg cccgaacggt cgttgcgacc ggaacgcttg cgccgatcag
    11761 ggcgcttctc gcatagcgct ctctggcggc gctcgggaac acgaacagca gcgcgtcctc
    11821 ggaggtgacg gtccggccga cgatccggta gtggtaggtc ttgttcgcca actggtcgtg
    11881 gttctcggtt ccccggtcgt actccaggaa gaacgagatc tggcggtccc aggagcggat
    11941 gcggccgatg ccatcggggt tgatgacccc gtcccaccgg cgcgccgccc gtcgctcccc
    12001 gatccactcg agctcccacc cgcggcctcg gcacgcccgg gtgagccgcg cgaagaagcc
    12061 gttgcactcc acccggtgcc ggagcctcgg gctacccacg acgtcgaaga ttcgggcctt
    12121 cttgaaggtg aagtccttga agtcgatctc ctggcgcg
//
LOCUS       DASQQQ010000260         2786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_159085, whole genome shotgun sequence.
ACCESSION   DASQQQ010000260 DASQQQ010000000
VERSION     DASQQQ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159085"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VE646_09905"
     CDS             <1..293
                     /locus_tag="VE646_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HZD80337.1"
                     /translation="VNVHLVQILDRHGIRIRTYERGVEAETLACGSGSISSALALAAA
                     GQVESPVRVETRSGEVLTIRFERDGDGGFTDLELEGPAREVYRAELSPEASD"
     gene            332..1414
                     /gene="mutY"
                     /locus_tag="VE646_09910"
     CDS             332..1414
                     /gene="mutY"
                     /locus_tag="VE646_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HZD80338.1"
                     /translation="MAWYDAHRRDLPWRRTEDPYRVWVAEVMLVQTRVDTVIPYYEQF
                     LERFPDVRSLAEADLDDVFKAWEGLGYYARARNLHRAAGLIAREHDGRLPGTAEALRR
                     LPGIGPYTAAAIASIAFGQPAPAVDGNVRRVISRLYDRAEATPAELAGLARPWVEGGR
                     PGDVNQALMDLGATICVPRAPLCPECPLEGGCLARERGTAAERPGRRKSRPRPHYEIA
                     VGVIWRDGRILIAKRPAEGLLGGLWEFPGGKREPGEDPEATVVREVREELGLLVEPGS
                     KIAAVDHAYTHFEITLHAFHCRPRAGTAEPRASEDLAWVRPDELDDYAFPAANRRIIE
                     TLGKGPEDDAPSAPVRRASGRRAEPR"
     gene            complement(1526..1915)
                     /locus_tag="VE646_09915"
     CDS             complement(1526..1915)
                     /locus_tag="VE646_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80339.1"
                     /translation="MAERDSLPRRAGAGRHLYPGLVLAQTLQSFELFVYGHLEVVPGV
                     SITPEYFVLFSLTGLLLMGALIPSVAHGRGWALRLAKTFAVLEVLNGAGHIMVPIVQW
                     EYYPGLWTAPIILIFAAALGRSLRHVP"
     gene            complement(1926..>2786)
                     /locus_tag="VE646_09920"
     CDS             complement(1926..>2786)
                     /locus_tag="VE646_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HZD80340.1"
                     /translation="QEGDVQIKGYPIRLPLDVQLVFTANPEDYTARGKIITPLKDRIG
                     AEVTTHYPRELDDGIRITGQESWTERGSRPTQIPYLVREVIERIAFEAREDPRVDRRS
                     GVSQRLPITVLESVVSNAERRAILAGEDRATPRMADIYAALPAITGKLELEYEGELQG
                     GARVAADLIGRAADATFDEYFHEEDAAPVVEYFDGGGMLQVSDVSGTDACLKGFGTVP
                     GLVELAETAVLEDEKGPASLVAASELILEGLVAKRKLSRNEGGRYSRRRGRPRPPKHE
                     QGPGGGDLFT"
BASE COUNT          403 a          987 c          998 g          398 t
ORIGIN      
        1 gcgtgaacgt ccacctggtc cagatcctgg accgccatgg gatccggatc cgcacctacg
       61 agcgcggggt ggaggccgag acgctggcct gcggcagcgg atcgatctcc tcggcgctcg
      121 ccctggccgc ggcggggcag gtcgaatcgc cggtccgcgt cgagacgcgg agcggcgagg
      181 tcctgacgat ccggttcgag cgggacggcg acggcggctt cacggacctc gagctggaag
      241 ggcccgcccg ggaggtgtat cgggccgagc tgtcgccgga ggcgtccgac tgagcgcccc
      301 gccgcccgat ccggcgacag cctcgcggct gctggcctgg tacgacgccc atcgccgcga
      361 cctcccctgg cggcgcacgg aggacccgta ccgggtgtgg gtggccgagg tgatgctggt
      421 ccagacgcgg gtggacacgg tcatccccta ctacgagcag ttcctggagc gatttcccga
      481 tgtccggtcg ctggccgagg ccgatctgga cgacgtgttc aaggcgtggg aggggctggg
      541 ctactacgct cgcgcccgca acctgcaccg ggcggccggt ctgatcgccc gcgagcacga
      601 cggccgcctg ccggggacgg cggaggcgct gcggcgactt ccggggatcg ggccctacac
      661 cgccgccgcg atcgccagca tcgccttcgg ccagccggcc ccggcggtgg acgggaacgt
      721 gcggcgcgtg atcagccgat tgtacgaccg cgccgaggcg acccccgcgg agcttgccgg
      781 cctggcccgg ccctgggtcg agggcgggcg gccgggcgat gtgaaccagg cgctgatgga
      841 cctgggcgct accatctgcg ttccccgagc gcccctctgt cccgaatgcc ccctggaggg
      901 cggctgcctc gcccgggagc gtggcacggc ggccgagcgc cccgggcgcc ggaagagccg
      961 cccgcgtccc cactacgaga tcgcggtggg tgtgatctgg cgcgacgggc ggatcctgat
     1021 cgcgaagcgg ccggcggagg ggctcctggg cgggctgtgg gagttcccgg gcggcaagcg
     1081 ggagccgggt gaggatccgg aggcgacggt ggtgcgcgag gtgcgggagg agctggggct
     1141 actggtggag cccgggtcga agatcgcggc cgtggaccac gcctacaccc acttcgagat
     1201 cacgctccac gccttccact gccgacctcg cgccggcacg gccgagccgc gggcgtccga
     1261 ggatctggcc tgggtccggc ctgacgagct ggacgactac gccttccccg cggccaaccg
     1321 tcggatcatc gagacgctcg ggaaggggcc cgaggacgat gcgccgtcgg ctccggtccg
     1381 ccgagcgtcc ggccgccgag cggagccacg gtagcgggga actgctagtg ttcggccgta
     1441 gtgattgtga ggcttgtgcc gcgccgaggt gtcctctacc acggaatcac cgtggccgac
     1501 aactagccgg tggccgaccc ccccttcagg gcacgtgtcg aaggctgcgc ccgagagcgg
     1561 ccgcgaagat gaggatgatc ggcgcggtcc agaggccggg atagtactcc cactgcacga
     1621 tcggcaccat gatgtgcccg gcgccgttga gcacctcgag cacggcgaag gtcttggcga
     1681 gccgcagcgc ccaaccccgg ccgtgggcca ccgaagggat gagggccccc atcagaagaa
     1741 ggccggtgag gctgaagagg acgaagtact ccggagtgat ggagaccccg ggcaccacct
     1801 ccaggtggcc gtagacgaac agctcgaagc tctggagcgt ctgagccagg acgaggccgg
     1861 ggtagagatg gcggcccgcg ccggcgcgcc gtggcaacga atcccgttcg gccacgccgg
     1921 cgagactatg tgaagagatc tccacccccc ggtccctgct cgtgcttcgg cgggcgcggg
     1981 cgcccacgcc gacgcgaata tcggccgccc tcgttccggc tcagcttccg tttcgccacg
     2041 agcccttcca ggatcagctc gctggcggcc accagggacg ccggcccctt ctcgtcttcc
     2101 agcacggctg tctcggcaag ctccaccaga cccgggacgg tcccgaatcc cttcaggcac
     2161 gcgtccgtac cggagacgtc cgagacctgg agcatgccgc ccccgtcgaa gtactcgacg
     2221 accggcgcgg cgtcctcctc gtggaagtac tcgtcgaacg tcgcatccgc cgcccggccg
     2281 atcaggtcgg cggccaccct ggcgccgccc tgaagctcgc cctcgtactc cagctccagc
     2341 ttacccgtga tcgccggcaa cgccgcgtag atgtccgcca tccggggcgt cgcccggtcc
     2401 tcgcccgcga ggatggcgcg gcgctccgcg ttcgagacca cgctctccaa caccgtgatc
     2461 ggcaggcgct gggagacccc ggaacgtcga tcgacgcgcg gatcctcccg cgcctcgaac
     2521 gcgattcgct cgatcacctc ccgcaccagg taggggatct gggtcggtcg actgccccgt
     2581 tccgtccatg actcctggcc ggtgatccgg attccgtcat ccagctcccg cggatagtgg
     2641 gtggtcacct cggccccgat ccggtccttc aacggggtga tgatcttccc ccgagccgtg
     2701 tagtcctcgg ggttcgccgt gaagacgagc tgcacgtcga gcggcaggcg gatcggatag
     2761 cccttgatct ggacgtcccc ctcctg
//
LOCUS       DASQQQ010000261         8550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_293911, whole genome shotgun sequence.
ACCESSION   DASQQQ010000261 DASQQQ010000000
VERSION     DASQQQ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8550
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293911"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2078
                     /gene="leuS"
                     /locus_tag="VE646_09925"
     CDS             <1..2078
                     /gene="leuS"
                     /locus_tag="VE646_09925"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HZD80341.1"
                     /translation="RLHTCDPEYYRWTQWLFVKLFERGLAYRRTAPANWCPNDQTVLA
                     NEQVVNGACERCGTSVVRKDLTQWFFKITDYAQRLLDDVDQLVDWPERVIRMQRNWIG
                     RSEGAQVTFEIAETGDGVEVFTTRPDTLWGVTFFVFALEHPLVLELARAGGTEPQARA
                     MLDALQATPLTNREQAESREGVDLGVHAVNPVNGEKVPCFVAPYVLMEYGTGAVMGVP
                     AHDQRDFEFARAHDLPIRVVVRPEGEARDADGMTQAYDHDGVMVNSGPFDGVRSPESI
                     GQVIAWLEEQGKGSAAVSYRLRDWLISRQRFWGAPIPIVYCPEHGEVPVPEDQLPVVL
                     PEDVDFQPGGESPLARHPTWKHAICPTCGQEAVRDTDTMDTFVDSSWYFFRYCSPGEG
                     ERAFDPERVAGWMPVDQYTGGVEHAILHLLYSRFFTKVLFDMGLVGFTEPFPRLMNQG
                     QVIYGGASMSKSKGNIVEPMPLVERWGADTMRITILFAGPFEDDIDWKLIAPDPGKRP
                     PMNSWLGRVHAAVFEAAEREAPDPERLSRLVHRTIRDVSEDLDRFRLNVAISKLMVLT
                     NEIRSVLDEGRGAREAASALTLMLAPLAPFLAEELWREALHHPISVHSAPWPEFDPEL
                     ARQERVTMIVQVDGKVRDRIGIDADASEETCRRAALGSEHARRYLEGREPRRVIVRPP
                     KLVNIVTGG"
     gene            2195..3571
                     /locus_tag="VE646_09930"
     CDS             2195..3571
                     /locus_tag="VE646_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012411967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HZD80342.1"
                     /translation="MDEESWFEPYKIKAVEAIPITIREERRQALEKAGWNTFLLRSDQ
                     VTIDLLTDSGTNAMSDRQWAALMIGDEAYAGSRNFYRLEETVRRIYGYDFMVPTHQGR
                     GAEHIVSQMLIRPGDMVPGNMYFTTTRLHQELAGATFVDVIVPEAHDPESRHPFKGDV
                     DLEKLEKVIEQADRVPYLSVAATVNMAGGQPISLSNLRQVRELAGRHGIPVVLDATRA
                     VENAYFIKEREPGQADRPVAQILRELCDLTDLCTMSAKKDALVNIGGWIGLRDPELAA
                     EARNLCVVYEGLHTYGGLAGRDLEAIAVGIEESVDDDHIRARVLQVRELGRALERQGV
                     PIVEPIGGHAVFLDARRFYPHIPQEAFPAQRLAGEIYLESGTRTMERGIVSAGRDPET
                     GQHRHPALELVRITIPRRVYTSRHLAAVARGVEDVYRRRASTRGLEMTFEPKYLRFFQ
                     ARFRPLES"
     gene            3658..4287
                     /locus_tag="VE646_09935"
     CDS             3658..4287
                     /locus_tag="VE646_09935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HZD80343.1"
                     /translation="MSEGSLRERLSALSRGELIGLVAVLAISLGGAALWYVRSLPSPV
                     QVRAVGGAALPSITTPGPSPEPVPILVDVAGWVRRPGVYGFHEGDRIIDAIDAAGGAR
                     PGAQLDALNLAAPLADSTQVLVPRSAPRSSVGAVPGVVPAATAGAQINVNTASATELE
                     ALPGIGEVLAQRIVDYRTEHGPFSAVDDLLDVTGIGDVILGDIRDQVTV"
     gene            4880..5695
                     /locus_tag="VE646_09940"
     CDS             4880..5695
                     /locus_tag="VE646_09940"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HZD80344.1"
                     /translation="MVLRGCPPSSGTNTAERRGAASNPVVRAAQIFRTFLGRSIRRLF
                     PRREAGLLLGLALGDDSMLEPALIRDLQATGLGHLLVVSGENVAMVLAPVLGLALLLR
                     LSRVPTFTLGTATVVFFVVLTGAEPSVMRAGAMAVLALVGVLLGRPRSAASILVGAVL
                     VLLVMDPALVWSVGFQLSVAATAGMVALASPLSERLAVLPRPVALAAGTTLAAQAGVT
                     PILLFHFHEVPGSTLPANLLAFPAVSPALLLGLLAAAVGLAVLPAGRVPAGLA"
     gene            6474..6671
                     /locus_tag="VE646_09945"
     CDS             6474..6671
                     /locus_tag="VE646_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80345.1"
                     /translation="MPEFFQAVGASIAVVSVGQPNDYGHPVASVLESLRATGARVLRT
                     DQRGDIVVTFGPEGLLVESAP"
     gene            6706..7653
                     /gene="holA"
                     /locus_tag="VE646_09950"
     CDS             6706..7653
                     /gene="holA"
                     /locus_tag="VE646_09950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HZD80346.1"
                     /translation="MSPRGAPVVLLWGEDDFLLREAALEAIGPDLRAREVDAAEWQAG
                     ETADLATPSLFGDRRALIVSDARRLGESAQQELAAYLADPAPDAPLVLCATVPTRGKA
                     PAALVKLVKPVGEIREVKITRRDLPGWVMGRAKARGVQLAPDAAAALVETVGEEPGAL
                     DQAIVQLSTAFGGSRITREAVASQFRGLGDQHIWDLCDRAFGRDLPGAIRSVRTLLES
                     REEGLMILGGVASRLRDLLRVKALPEQLPPSELARAAGLRFEWQARRYREQARRFTLD
                     QLLAIHDRLVEADRSLKSGASDDVVLPMLVSVIADAATA"
     gene            complement(7655..7930)
                     /gene="rpsT"
                     /locus_tag="VE646_09955"
     CDS             complement(7655..7930)
                     /gene="rpsT"
                     /locus_tag="VE646_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018349310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HZD80347.1"
                     /translation="MANIRSQIKRNRQNERRRQRNKAVRSALKTSSKKVRTSMAEDDA
                     DTAREQLAEATRAYDKAAAKGVIHPRTAARHKSRLARSTAPVRAEAE"
     gene            7973..>8550
                     /locus_tag="VE646_09960"
     CDS             7973..>8550
                     /locus_tag="VE646_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZD80348.1"
                     /translation="MAPRRPYDSPGVTDTSLIRNFCVVAHIDHGKSTLADRLLELTHA
                     VAERQMREQYLDKMDLERERGITIKAQAVRLSHRIDGRLFELNLIDTPGHVDFTYEVS
                     RSLAACEGAVLLVDAAQGIEAQTLANYHLARDAGLTIIPALNKIDLPAADPDRAAAEL
                     VGIIGGSANDVLRLSAKTGEGVDDLLETLVKVV"
BASE COUNT         1159 a         2908 c         3124 g         1359 t
ORIGIN      
        1 ggcgcctgca cacctgcgat ccggagtact accggtggac gcagtggctg ttcgtgaagc
       61 tgttcgaacg gggcctggcc taccggagga cggccccggc gaactggtgt ccgaacgacc
      121 agaccgtgtt ggccaacgag caggtggtga acggggcgtg cgagcgctgc ggcacctcgg
      181 tcgtgcgcaa ggatctcacc caatggttct tcaagatcac cgactacgcg cagcggctgc
      241 tggacgacgt ggatcagctg gtggactggc cggagcgagt gatcaggatg cagcggaact
      301 ggatcggccg gtccgagggc gcgcaggtca ccttcgagat cgccgagacc ggcgatggcg
      361 tcgaggtctt caccacccgc cccgacacgc tgtggggcgt gacgttcttc gtgttcgccc
      421 tggagcaccc gctggtgctg gagctggcga gggcgggggg aaccgagccc caggcccgag
      481 ccatgctgga cgcgctgcag gccacgcccc tcacgaaccg cgagcaggcc gagagccggg
      541 agggcgtgga tctcggcgtg cacgcggtga accccgtgaa cggcgagaag gtgccgtgct
      601 tcgtagcgcc ctacgtgctg atggagtacg ggaccggggc ggtgatgggg gtcccggcgc
      661 acgaccagcg cgacttcgag ttcgcccgcg cgcacgacct gccgatccgg gtggtggtgc
      721 ggcccgaggg cgaggctcgg gatgcggacg ggatgaccca ggcctacgac cacgacggcg
      781 tgatggtgaa ctcgggcccg ttcgatgggg tccggtcgcc ggagtcgatc gggcaggtga
      841 tcgcctggct ggaggagcag ggcaagggct cagccgccgt gagctaccgg ctccgggatt
      901 ggctgatcag ccggcagcgg ttctggggag cgccgatccc catcgtgtac tgccccgagc
      961 acggcgaggt gccggtgccc gaggaccagc tgcccgtggt cctgcccgag gacgtggact
     1021 tccagcccgg cggggagtcg ccgctggctc gccatcccac ctggaagcac gccatctgcc
     1081 ccacatgcgg gcaggaggcg gtgcgcgaca ctgacacgat ggacaccttc gtggactcgt
     1141 cctggtattt cttccgctac tgctcgcccg gggagggcga acgcgccttc gatccggaac
     1201 gggtggccgg gtggatgccg gtcgaccagt acacgggggg cgtggagcac gcaatcctcc
     1261 acctgctcta ctcgcggttc ttcaccaagg tgttgttcga tatgggcctg gtgggcttca
     1321 cggagccttt cccccggctg atgaaccaag gacaggtgat ctacgggggc gcgtcgatga
     1381 gcaagtcgaa gggcaacatc gtcgagccca tgccgctggt ggagcgctgg ggcgcggaca
     1441 cgatgcggat cacgatcctg ttcgccggac cgttcgagga cgacatcgac tggaagctga
     1501 tcgcgcccga tcccggcaag cgtccaccga tgaactcgtg gctgggccgg gtccatgcgg
     1561 ccgtgttcga ggcggccgaa cgagaggcac ccgatccaga gcggctgtcg cgtctggtgc
     1621 accgaacgat ccgggacgtg agtgaggacc tggaccgctt ccgcctcaac gtggcgatct
     1681 cgaagctcat ggtgctgacc aacgagatcc gctcggtcct ggacgagggg aggggggcac
     1741 gggaggcggc ctccgccctc accctgatgc tggccccgct cgccccgttc ctggccgaag
     1801 agctctggcg cgaggccctc catcacccga tcagcgtgca ctcggcaccc tggccggagt
     1861 tcgatccgga gctcgcccgc caggagcgcg tgacgatgat cgtccaggtg gacggcaagg
     1921 tgcgggaccg gatcgggatc gatgccgacg catcggagga gacctgccgc cgggccgctc
     1981 tcggctccga gcacgctcgc cggtacctgg agggacgcga gccccgtcgc gtcatcgtcc
     2041 ggccgcccaa gctcgtcaac atcgtcaccg gcggctgacg ccgcccggcc cgctccccgc
     2101 gggccgttcg actcccctat caaccccgag ccagtcgcag gctgaccgat ggtggcgacc
     2161 gagcggtcgg acgcggggtg aggaggtgcc gtcggtggac gaggagtcct ggttcgagcc
     2221 ctacaagatc aaggcggtcg aggcgatccc catcaccatc cgggaggagc gccgacaggc
     2281 gctggaaaag gctggatgga acacgttcct gctgcgctcc gatcaggtga cgatcgacct
     2341 gctgaccgac tcgggaacca acgcgatgag cgatcgacag tgggccgctc tgatgatcgg
     2401 tgacgaggcg tacgcgggga gccggaactt ctaccggctg gaggagacgg tccgccggat
     2461 ctacggctac gacttcatgg tgcctaccca tcagggtcgt ggtgccgagc acatcgtgag
     2521 ccagatgctg atccgacccg gggacatggt cccgggcaac atgtacttca ccaccacgcg
     2581 tctgcaccag gaattggccg gcgccacgtt cgtggacgtg atcgttcccg aggcccacga
     2641 ccccgagagc cgtcacccct tcaaggggga cgtggatctg gagaagctgg agaaggtgat
     2701 cgagcaagcg gaccgcgtcc cgtacctgtc ggtcgccgcc accgtgaaca tggccggtgg
     2761 ccagcccatc tcactgtcga acctccggca ggtccgggag ttggccggtc gccacggcat
     2821 cccggtggtg ctggacgcca cccgggcggt agagaacgcc tacttcatca aggagcgcga
     2881 gcccggccag gccgatcgac cggtggccca gatcctgcgc gagctatgcg acctcaccga
     2941 tctctgcacg atgtccgcga agaaggacgc cttggtgaac atcggcgggt ggatcgggct
     3001 ccgggacccg gagcttgcgg ccgaggcccg gaacctgtgc gtggtctacg aggggctcca
     3061 cacgtatggt gggctggccg gccgggacct ggaggccatc gccgtgggga tcgaggagtc
     3121 ggtggacgac gatcacatcc gggctcgcgt gctgcaggtg cgcgaactgg gacgggcgct
     3181 ggagcgccag ggcgtgccga tcgtcgagcc gatcggcggt catgccgtgt tcctggacgc
     3241 ccgccgcttc tacccgcaca tcccgcagga ggcgttcccc gcccagcgcc tggcgggcga
     3301 gatctacctg gagtccggca cccggaccat ggaacggggg atcgtctcgg cgggacggga
     3361 tcccgagacc ggtcagcatc gccatccggc cctggagctg gtgcggatca cgatccctcg
     3421 ccgcgtttac acctcgcgac acctggcagc cgtggcccgg ggcgtggagg acgtctatcg
     3481 gcgccgagcc tccacccgcg ggctcgagat gaccttcgag cccaagtacc tgcggttctt
     3541 ccaggcacgg ttccggccgc tcgagtcctg aactcgcgta cgtgtgacct gcttcacggc
     3601 gcgccgacac cggtcgggct agcttgcggg atcttgccct cgactcgtgg aggctggatg
     3661 tcggaagggt ccctgcggga gcgactgtcc gcgctgtcgc gcggtgagct catcggactg
     3721 gtcgccgtgc tggcgatctc gctcgggggc gcagcgctct ggtacgtgcg gtcgctaccg
     3781 agcccggtgc aggtgcgggc cgttggtggg gccgccctgc cgtcgatcac cacgcccggc
     3841 ccatcgcccg agccggtacc catcctggtg gacgtcgccg gctgggtgcg tcgcccgggc
     3901 gtctacgggt tccacgaagg cgaccgcatc atcgatgcga tcgatgccgc cggtggtgcc
     3961 cggcccggcg cccagctgga cgccctgaac ctggccgcgc cgctcgccga cagcacccag
     4021 gtcctggtcc cacggtcggc cccgcgctcg agcgtcgggg cggtgcccgg cgtggtcccc
     4081 gccgcgacgg ctggagccca gatcaacgtc aataccgctt cggccaccga gctcgaagcg
     4141 ctgcccggga tcggggaggt cctggcacag cggatcgtcg actaccgaac ggaacacggg
     4201 cctttctcgg ccgtggatga tctcctggac gtgaccggga tcggcgatgt gatcctcggc
     4261 gatatccgcg accaggtcac ggtgtgagca cgtggctgtt gcccggagcg gccgggctct
     4321 tctggagcgg tctgttgatc tgcacggtcg tccccgatcg ggtgccgccc tgggtgctgc
     4381 tggggctggg gaccgtcctc ctcggactgg ccgccgccct ggctcccggc cgagtgctgc
     4441 gggatccggt cctcgaggcg ggtctggtcc gggcggcgga ggagcctcgg tcgctcgccg
     4501 cactgggccc gagtcggttg gctccgggtc gagggccgcc cggggccgcc ctggtctgtg
     4561 cggcggtagc cgtcctgatc ctcggcgccg ggtggggggg cctccacgcg gcccgaacgc
     4621 ggtcctcgct cctggcacgg ctggcccctg cccgggtgag cttggtcggg tccctgcgga
     4681 ccgatccatc gtctcgcccg tccggatggt ccgcgctggt ggacatccgg cgggtggagt
     4741 gggccgaggg tgcggcgaac ctccacggcg tgctctgggt gaacggtcgg gggcgccctc
     4801 cgggtgccgt tcgcggggat tcctgtcgct gtccgggacg ctggagcgct cggatcccag
     4861 cgaccccttc ggcaggtcca tggtgctccg ggggtgcccg ccgagctcag gaacgaacac
     4921 cgcggagcgg cgaggagcgg cctcgaaccc ggtcgtccgg gccgctcaga tcttccggac
     4981 cttcctgggt cgctcgatcc gccgactctt cccccggcgg gaagcgggcc tgctcctggg
     5041 gctggccctg ggggacgact cgatgctcga gcccgccctg atccgcgacc tccaggccac
     5101 cggcctgggg cacctcctgg tcgtctctgg ggagaacgta gccatggtgc tcgccccggt
     5161 cttggggctg gcactgctgc tccggctctc gcgcgtgccg accttcaccc tggggacggc
     5221 taccgtcgtc ttcttcgtgg tgctgaccgg cgccgaaccc tcggtgatgc gggccggcgc
     5281 gatggccgtg ctggctctcg tgggggtcct gctgggcaga ccgcggagcg cggcatcgat
     5341 cctggtcggc gccgtcctgg tcctgctggt catggatccc gccctcgtct ggtcggtggg
     5401 cttccagctc tcggtggcgg ccaccgccgg catggtggcg ctggcctcac cgctgtccga
     5461 gcggttggcg gtcctcccgc gaccggtggc gctcgctgcc gggaccacgc tggcggccca
     5521 ggccggggta actcccatcc tcctgttcca cttccacgag gttccagggt ccaccctgcc
     5581 cgccaacctg ctggcgttcc cggccgtgtc cccggcgttg ctgctcgggc tgctggctgc
     5641 cgccgtgggg ctcgcggtcc ttcccgccgg ccgggtcccg gccgggctgg cctgatcccc
     5701 atgcgctacc tggaggccgt ggccgatcgg ctggcgacgg cgccggtccc ccggatcacg
     5761 ggcggtggcg ccggaacgct gatcggaggt ctgctcgcgg tgggcgcggt cgcatggtgg
     5821 ctccgtacgg gccgcccgct gccgaaggtg cggtggtggc gatggcggcg ctgctgccgc
     5881 tggtggtgtg gtcctcggcc ctctcggctg gcccgccgtc cgggttgcag gtgcggttcc
     5941 tcgacgtggg acagggcgat gccgctctgg tcacctcgcc cagcggggca cggctgctga
     6001 tcgatgccgg acccgatccc gagcaggtag ccaccgagct ctcggccctg ggcgtgaccc
     6061 gcctggacgc cgtgtcgcca cccatccgca tgccgaccac atcgagggct tccccgccgt
     6121 cttcgcccgg ttcccggtcg gcctcgccct cgagcccgga tgcgacgagc cctcaccctc
     6181 gtacaccgac ctgctccggg ccatccggga ggaagaactg cccgtccagc acccgcgggc
     6241 cggcgaccgg atctgggtgg gcgatctgtt gctcgacgtc ctggcgccgc atgggtgctt
     6301 cacgggaacc gactcggacc cgaacaacga ctcgttggtg atccgtctgg cctaccggag
     6361 cgacgcggtg ctcttctcgg gggatgccga ggtccccgag caggaggcgc tgctccagga
     6421 cgggacggac ctgcggaccc agctgttcaa ggcatcacgg tggagacacg tcgttgccgg
     6481 agttcttcca ggctgtgggg gcttccatcg cggtggtgag cgtcgggcag ccgaacgact
     6541 acggccaccc cgtggcgtcg gtgctggagt cgctccgggc cacgggagcg cgcgtgcttc
     6601 gcaccgacca gcggggagac atcgtggtca ccttcgggcc ggagggtctg ctcgtagaat
     6661 ccgccccatg acgcgcgatg gacgtgcgcg caagggggcg ggtcgatgag ccctcggggt
     6721 gcccccgtgg tcctgctgtg gggggaggac gacttcctcc tgcgggaggc ggccctcgag
     6781 gccatcggac cggacctgcg ggcccgcgag gtggacgccg ccgagtggca ggcgggagag
     6841 acggccgatc tggccacccc ctcgctcttc ggcgaccgtc gggcgctgat cgtgagcgat
     6901 gcccgccggc tgggcgagag cgcgcagcag gagctggcgg cgtacctggc cgatccggct
     6961 ccggatgcgc cgctcgtgct ctgcgcgacc gtccccacgc gggggaaggc cccggccgcg
     7021 ctggtgaagc tggtgaagcc ggtcggcgag atccgcgagg tgaagatcac acgacgagac
     7081 ctccccgggt gggtgatggg acgggccaag gcccgggggg ttcagctcgc gccagacgcg
     7141 gcggcggcgc tggtcgagac ggtgggggag gaacccgggg ccctggacca ggccatcgtg
     7201 cagctctcga ccgccttcgg cgggagtcgc atcacccgtg aggcggtggc cagtcagttc
     7261 cgggggctgg gcgaccagca catctgggac ctgtgcgacc gggccttcgg ccgggacctg
     7321 ccgggcgcga tccggtcggt ccgcaccctg ctcgagtcac gagaggaggg gctgatgatc
     7381 ctggggggcg tcgcctcgcg cctgcgggac ctgctgcggg tgaaggccct ccccgagcag
     7441 ctgccgccct cggaactggc ccgggcggcc ggcctgcgct tcgagtggca ggcgcgccgg
     7501 taccgtgagc aggctcgacg gttcacgctg gatcagctcc tcgccatcca cgatcgcttg
     7561 gtggaggccg accggtccct gaagtccggg gcctccgatg acgtggtgtt gccgatgctg
     7621 gtgtcggtga tcgcggacgc cgcgacggcc tgacctactc ggcctcggca cgcaccgggg
     7681 cggtcgatct ggccagtcgg gatttgtgcc gggccgccgt gcgaggatgg atcacgccct
     7741 tggcggcggc cttgtcgtac gcgcgggtgg cctcggcaag ctgctcccga gccgtgtcgg
     7801 cgtcgtcctc ggccatcgag gtgcggacct tcttggagga ggtcttcagg gccgagcgca
     7861 cggccttgtt ccgctggcgc cgccgctcgt tctggcggtt gcgtttgatc tgggacctga
     7921 tgttcgccac ggcgaccgag tgtaccagcg gcgacgcacc gcccggcccc tcgtggcgcc
     7981 tcggcgcccg tacgatagcc ctggcgtgac cgacaccagc ctgatccgca acttctgcgt
     8041 cgtggcgcac atcgaccacg gcaagtccac gctggccgac cgcctgctgg agctcacgca
     8101 cgccgtggcc gagcgccaga tgcgcgagca gtacctggac aagatggacc tggagcgcga
     8161 gcggggcatc accatcaagg cccaggcggt ccgcctgtcc caccgcatcg atggccgtct
     8221 gttcgagctg aacctgatcg acacgcccgg gcacgtggac ttcacgtacg aggtctccag
     8281 gtccttggcg gcgtgtgagg gcgccgtgct gctggtcgac gcggcccagg gcatcgaggc
     8341 ccagacgctc gcgaactacc acctggcgcg cgacgcgggg ctcacgatca tcccggccct
     8401 gaacaagatc gacctccccg cggccgaccc cgatcgggcc gcagccgagt tggtcggcat
     8461 catcggcggc tccgccaacg atgttctccg tctgtccgcc aagacgggcg aaggagtcga
     8521 tgacttgctc gaaaccctgg tcaaagtggt
//
LOCUS       DASQQQ010000262         4060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_16327, whole genome shotgun sequence.
ACCESSION   DASQQQ010000262 DASQQQ010000000
VERSION     DASQQQ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16327"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..833)
                     /gene="arsM"
                     /locus_tag="VE646_09965"
     CDS             complement(72..833)
                     /gene="arsM"
                     /locus_tag="VE646_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HZD80349.1"
                     /translation="MNEVREQIREHYAAAARRVSIDTPAAGSTGCSGGSGCCGGTTSS
                     DPSLYGGDELEQVPDAAALASLGCGNPTAVAELREGERVLDLGSGGGIDVILSARRVG
                     PSGIAYGLDMTDEMLELARRNAAEAGVGNARFLKGYMEEIPLPDQSVDVVISNCVINL
                     STDKPRVFDEMARVLRPSGRIGISDIVSADDLTPQQRAERGSYVGCIAGALSFAEYEA
                     ELTRAGFGSIELVPTHEVTDGMVSAIVRARKRVSS"
     gene            complement(830..1186)
                     /locus_tag="VE646_09970"
     CDS             complement(830..1186)
                     /locus_tag="VE646_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZD80350.1"
                     /translation="MKREMAVIQPPAACCVPLGDAAMSAEEAEVTARVFKALSDPGRV
                     RIVNLLASTGEAVCVCDLTPEIGLSQPTVSFHLKKLAEVGLVRREKRGVWAYYAVDRD
                     LLARLATVFNLEGARG"
     gene            complement(1339..2844)
                     /locus_tag="VE646_09975"
     CDS             complement(1339..2844)
                     /locus_tag="VE646_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HZD80351.1"
                     /translation="MAQDRWPAVHYGRAEPEGVIGLRMTRGFMVRAAISSVVLSFVLG
                     AIGAAVAAAAVPTVTASLVQRIDTSSFSPASPDPSGVVYLPGRDRLEISDSEVDETTG
                     AGYHGVNLWQITRSGNVTDTGTTMGYPSREPTGLGYAPASGKLFVSDDTKRRIYVVRP
                     GTDGRFGTADDVVSFIDAGAYGSTDTEDPEFDPATGHLFFLDGVGTEVYDIDPVNGVF
                     GDGDDSMTHFDIGKLGPTDWEGLSSDPSQGTLLVGARKDKKIYEITKTGTLVRIIDAS
                     GISGMRWLSGLALAPASDDPTRMDYWIVDRGVDNGSNPSENDGKLFELTIGEGGGGGG
                     GGSVLGISGFSPTSGPTGTSVTITGSAFQDVTVVKFNKSKAVFTIVSDTEITATVPGG
                     ATTGLVKVKTPTDIAKSSEPFTVTSGGSTTGLSISGFSPTSGPVGTSVTITGSGFLDV
                     TIVKFDTTKASFTIDSDTQITTTVPAGATTGKIKVKTPSNIARSSTLFTVL"
     gene            complement(2942..>4060)
                     /locus_tag="VE646_09980"
     CDS             complement(2942..>4060)
                     /locus_tag="VE646_09980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZD80352.1"
                     /translation="PPRAALLAGDLARPTGGNLYDDHLIRAAPAHGFCLHPHAVPDRL
                     PAALRGAPDALRRAARPPTAVLVIDSIAARGLGPALARRPPPVPVVALLHQPPGGGDG
                     SVLRRGLGRGLDRLAYRRAGRLIVASTWLGDRLVRAGFDPASIRVVPPGCDVRATQGG
                     PLPDLRRGRAAAILCVANWTAQKGILELVEAFARLEPGLATLHLVGDPRAVPAYGARV
                     RRRLARSDLQGRVVVHGPRPPGEASRLRSAADVFALPAHRETYGMAWAEALRDGLPVV
                     GWRSGHLPELVRDGSEGLLLPEGDLAGLTSALDRLCRDLGLRDRMRHSSRVRGSTLPT
                     WSDTARRFFDVLREALELGPPAFQRSPHRSWCDRWRRS"
BASE COUNT          581 a         1503 c         1394 g          582 t
ORIGIN      
        1 gccgggcacg tgccgaccgt cgcccacatc cgggagctgc tgggctcgcg gggctagtcg
       61 gagcgagctc ctcaggacga cacgcgcttg cgggcccgga cgatcgccga caccatgccg
      121 tcggtgacct cgtgcgtcgg caccagctcg atcgagccga accccgcccg ggtgagctca
      181 gcctcgtact ccgcgaagga gagggctccg gcgatgcatc ccacgtacga accacgctcc
      241 gcccgctgct gcggcgtgag gtcgtcggcc gacacgatgt cgctgatgcc gatgcgaccg
      301 ctggggcgca ggacccgggc catctcgtcg aacacccgag gcttgtccgt cgacaggttg
      361 atcacgcagt tggagatcac gacgtccacg ctctgatcgg ggagggggat ctcctccatg
      421 tatcccttca ggaaccgcgc gttgccgacc cccgcctcgg ccgcgttgcg gcgcgccagc
      481 tcgagcatct cgtccgtcat gtccaagccg taggcgatgc ccgacggtcc gacccggcgt
      541 gcggacagga tcacgtcgat ccctccgccg gagccgaggt cgaggacgcg ctcaccctcc
      601 cgcagctcgg ccaccgccgt ggggttcccg cagccgaggc tcgccagcgc cgccgcatcc
      661 ggcacctgct cgagctcatc ccccccgtag aggcttgggt ccgacgacgt cgttccgccg
      721 cagcaacccg atccgccgct gcacccggtc gacccggccg caggggtgtc gatcgatacg
      781 cggcgtgcgg ccgccgcgta gtgctcccgg atctgctccc gcacctcgtt catcccctcg
      841 ccccctccag gttgaagacg gtcgccaacc gcgccagcag gtcgcgatcc accgcgtagt
      901 aggcccagac gccacgcttc tcccgtcgca cgagcccgac ctcggcgagc ttcttcaggt
      961 ggaagctcac ggtcggctgc gacagcccga tctccggggt gagatcacac acgcacacgg
     1021 cctcaccggt gctcgcgagc aggttcacga tccgcacgcg cccggggtcg gacagggcct
     1081 tgaacacccg cgcggtcacc tccgcctcct cggccgacat cgccgcatct cccagcggca
     1141 cgcagcacgc cgccggtggc tggatcaccg ccatctcgcg cttcacgcgc gcctccctcg
     1201 tatcgacgtt cgtcgatgga tactaaccga tatatcgacg ttcgtcgata cgtacagcat
     1261 tccgagcgcg cggaagactg gtcccagggc gacccggacc ggtgcgcgcc gagccgcacc
     1321 gcgccgcctc gagcggtctc agaggaccgt gaagagggtg gagctccgag cgatgttgga
     1381 cggggtcttg accttgatct tcccggtggt ggccccggcc ggcacggtgg tcgtgatctg
     1441 ggtgtccgag tcgatcgtga aggacgcctt ggtcgtgtcg aacttgacga tcgtcacatc
     1501 gaggaagccc gacccggtga tcgtgacgga ggtgcccacc ggccccgagg tgggggagaa
     1561 cccggagatg ctgagcccgg tggtgctccc gccggatgtc acggtgaacg gctcggagct
     1621 cttcgcgatg tcggtgggtg tcttcacctt caccagcccc gtggtggcgc cgccaggcac
     1681 ggtcgcggtg atctccgtgt cggagacgat cgtgaacacg gccttgctct tgttgaactt
     1741 cacgaccgtc acgtcctgga acgccgaacc ggtgatggtc acgctcgtcc ccgtcgggcc
     1801 ggaggtgggg gagaagccgg agatccccag gaccgagccg ccgcctccac caccgccccc
     1861 ttcgccgatc gtgagctcga agagcttccc gtcgttctcg ctggggttgg acccgttgtc
     1921 cacgccgcgg tccacgatcc agtagtccat ccgggtgggg tcgtcgctgg ccggggccag
     1981 ggccagcccg gacagccagc gcatcccgct gatcccggag gcgtcgatga tccgcaccag
     2041 ggtgccggtc ttcgtgatct cgtagatctt cttgtccttg cgggccccca ccagcagggt
     2101 gccctgactc gggtccgagc tcagcccctc ccagtcggtg ggcccgagct tgccgatgtc
     2161 gaagtgcgtc atgctgtcgt cgccgtcgcc gaacacgccg ttcaccgggt cgatgtcgta
     2221 gacctcggtc ccgaccccgt ccaggaagaa caggtggccg gtggccgggt cgaactccgg
     2281 gtcctccgtg tcggtgctgc cgtaggcgcc cgcgtcgatg aagctcacca cgtcatcggc
     2341 ggtcccgaac cggccgtcgg tgcccggccg gaccacgtag atccggcgct tggtgtcgtc
     2401 ggagacgaac agcttgcccg aggccggcgc gtaccccagg ccggtgggct ccctcgacgg
     2461 gtaccccatg gtggtcccgg tgtccgtgac gttgccggac cgcgtgatct gccacaggtt
     2521 caccccgtgg tagccggccc cggtggtctc gtccacctcg gagtccgaga tctccagccg
     2581 gtcccgcccg ggcaggtaga cgacccccga gggatcggga ctggccgggc tgaacgagga
     2641 cgtatcgatc ctctggacca acgacgccgt caccgtggga acggcggcgg ccgcgacggc
     2701 ggccccgatc gcccccagca cgaaggacag caccacggac gagatcgccg cccgcaccat
     2761 gaacccccgg gtcatacgca acccgatcac cccctccggc tcggctcgac cgtagtggac
     2821 ggccggccac cggtcctggg ccatatgcac catagatggc acgcacttcg gaccaccccc
     2881 ggggctcgtg atgaggtccg ggccgtgggc gatggggcgg acctccgcta ccgatgcggc
     2941 gtcacgatct cctccaccgg tcgcaccaag atcggtgggg ggagcgctgg aatgcggggg
     3001 gtccgagctc cagtgcctct cgcagcacgt cgaagaacct tcgcgcggtg tccgaccacg
     3061 tgggcagcgt gctgcctcgt acccggctcg agtgacgcat ccggtccctg agcccgagat
     3121 ctcggcacag acgatcgagc gcggaggtca gcccggcgag gtcgccctcg ggaaggagca
     3181 ggccctcgct cccgtcccgc accagctcgg ggaggtggcc gctccgccag cccaccacgg
     3241 gcaggccgtc ccggagcgcc tcggcccagg ccatgccgta cgtctcccgg tgcgcgggga
     3301 gcgcgaacac gtcggccgcc gagcgcagcc gggacgcctc cccgggcggg cgagggccgt
     3361 gcaccacgac ccgcccctgc aggtccgaac gagccaggcg cctgcggacc cgcgccccgt
     3421 aggcgggcac cgctctcgga tcgcccacga ggtgcagggt ggccagcccg ggctcgaggc
     3481 gcgcgaaggc ttcgaccagc tcgaggatgc ccttctgagc ggtccagttg gccacgcaca
     3541 ggatcgcggc cgcgcggccc cggcgcaggt cggggagcgg tccgccctgg gtcgcccgga
     3601 cgtcgcaccc ggggggcacc acgcggatgc tcgccggatc gaaccccgcc cgcaccaacc
     3661 ggtcgccgag ccacgtgctc gcgacgatca gcctccccgc tcgccggtac gcgagtcggt
     3721 ccagcccgcg accgagcccc cgccgcagca ccgacccgtc cccaccaccg ggcggctggt
     3781 gcagcagggc caccaccggc accggcggag gacggcgggc gagggccggt cctagccccc
     3841 tggcggcgat gctgtcgatc acgaggacgg ccgtgggggg gcgcgcggcg cggcgcagcg
     3901 catccggcgc cccccgcagc gccgcgggca ggcggtcggg gaccgcgtgc ggatgcaggc
     3961 agaagccgtg ggctggcgcg gcccggatca ggtggtcgtc gtacaggttg ccgccggtgg
     4021 gccgcgccag gtccccggcc aggagtgcgg cgcgcggtgg
//
LOCUS       DASQQQ010000263         3174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_46276, whole genome shotgun sequence.
ACCESSION   DASQQQ010000263 DASQQQ010000000
VERSION     DASQQQ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46276"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VE646_09985"
     CDS             <1..1073
                     /locus_tag="VE646_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZD80353.1"
                     /translation="DFAGAIGALPLGHGHPAWRAAIHRQVDELGLVSNLYATGPQAEL
                     AARLAELLPVPEARVFFSNSGAEANEAALKLVRRWGLPRGRGRIVALDGSFHGRSVAT
                     LAATGQPAKRVPFEPLVDWFVHVPPGDLEAMDRAVGAETAAVLLEPVMGEGGVHPLEP
                     AYLRGIRQVCDERGALMMADEVQSGIGRCGAWLAVCTSDVVPDVVTLAKGLGGGLPIG
                     ATVTRADLALGPGEHASTFGGGPVVCAAALAVLETIEREHLLENARAQGERLRAAVRA
                     LEHPAVVEVRGAGLLNGIQLRGPDVHEVVLAMLERGVLATEAGPDVVRLSPPLVVTAA
                     EVDEAVAVLGSALDRLPARAAS"
     gene            1070..1546
                     /locus_tag="VE646_09990"
     CDS             1070..1546
                     /locus_tag="VE646_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HZD80354.1"
                     /translation="MTRDAGATKARRQQLILSLVARERLGSQDEIRARLQYLGLPTTQ
                     STVSRDVEELGLARVHDAEGVRYVVPAEGTTPGAMNLLRRALDEFALSFVRASQLLVV
                     RTPPGAANALAEAIDRANLADVAGTIAGDNTILVVPREGVAVRSVERALRGVQAGP"
     gene            1621..2808
                     /locus_tag="VE646_09995"
     CDS             1621..2808
                     /locus_tag="VE646_09995"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018503062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HZD80355.1"
                     /translation="MQRAVLAYSGGLDTSVTIRWLAEQGYEVFAVAVDVGQREDFALI
                     RERGALAGAAQVRVVDAVDRFAAEFLTPAVKANGLYEDKYPMVSALARPLIVDEVVAV
                     AREVGAQVVAHGCTGKGNDQVRFEVSFGVLAPDLRVLAPIRDDGIPREKAIALAGEWG
                     IPIAEVATSYSIDENLWGRTAECGPLEDPWAAPPEDAFARTAAPKDRPGEPTETVVAF
                     ERGVPVALDGRSLPLPELIRTVDGLGGRYGFGRVDMIENRRVGIKSRELYEVPGALAL
                     IGAHRALEDLTLEREVAHFKPGIEQRWADLVYDGLWFSPLRRALDAFVDATQSHVTGE
                     VRLSFAPGSCTVVGRWSEASLYDVGLATYGPQDAFDHRHAQGFVRLWGMPLKVWSARQ
                     GSG"
     gene            2805..>3174
                     /locus_tag="VE646_10000"
     CDS             2805..>3174
                     /locus_tag="VE646_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HZD80356.1"
                     /translation="MSGPSPRGEERGGPSERALWGGRFSSLPSPEALELGRSLSFDVR
                     LAEHDVRAGVAHVRGLVEGGLLDVGEAELLIGALREVGAEIAAGRFAFHPADEDVHSA
                     VERGVTERLGEIGEKLHAGRS"
BASE COUNT          385 a         1076 c         1279 g          434 t
ORIGIN      
        1 tggacttcgc cggcgcgatc ggagccctgc cgctcgggca cggccatccc gcctggcggg
       61 cggcgatcca ccggcaggtg gacgagctgg gactggtctc caacctgtac gcgaccggcc
      121 ctcaggccga gctcgcggcc cggctggcgg agctgctccc cgtcccggag gctagggtct
      181 tcttctcgaa ctcgggggcg gaggccaacg aggccgcgct gaagctggtc cgccggtggg
      241 ggctcccccg cggccggggc cggatcgtcg ccctggacgg atcgttccac ggtcggagcg
      301 tcgctaccct ggccgcgacc gggcagccgg ccaagcgggt ccccttcgag ccgctggtgg
      361 actggttcgt ccacgtgccg ccgggggacc tggaggcgat ggaccgggcc gtcggcgcgg
      421 agaccgccgc ggtgctgctc gagccggtca tgggcgaggg cggggtccac ccgctggagc
      481 cggcctacct gcgcggcatc cggcaggtct gcgacgagcg cggggccctg atgatggccg
      541 acgaggtgca gtccgggatc ggtcggtgcg gtgcgtggct ggccgtctgc acctcggacg
      601 tggtgcccga cgtcgtcacc ctggccaagg gcctgggcgg cgggctgccc atcggtgcca
      661 cggtgacgcg agccgacctg gccctcgggc ccggcgagca tgcgtccacg ttcgggggcg
      721 gtccggtggt gtgcgcggcc gccctggccg tgctggagac gatcgagcgt gaacacctgc
      781 tggagaacgc ccgggcccag ggcgagcgcc tccgggccgc cgtccgagcc ttggagcacc
      841 ccgccgtggt cgaggtccgc ggggcgggcc tgctcaacgg gatccagctc cgtggcccgg
      901 acgtgcacga ggtcgtcctg gccatgctgg agcggggggt cctggccacc gaggccggtc
      961 cggacgtggt gcgactctcg ccgccgctgg tggtcaccgc cgccgaggtg gacgaggcgg
     1021 tcgccgtcct cggatccgcg ctggaccggc tgccggcgag ggctgcttcg tgacccggga
     1081 cgccggagcc accaaggcca ggcgccagca gctgatcctc tccctggtgg cgcgcgagcg
     1141 cctggggagc caggacgaga tccgggcccg gctccagtac ctggggttgc ccaccacgca
     1201 gtccacggtc tcccgtgacg tggaggagct cgggctggcc cgcgtgcacg acgccgaggg
     1261 cgtccggtac gtcgtcccgg cggagggcac cacgcccggg gcgatgaacc tgctccggcg
     1321 ggcgctggat gagttcgccc tgtcgttcgt ccgggcgagc caactcctgg tggtgcggac
     1381 gccgccgggc gccgccaacg cgctggccga ggcgatcgac cgggccaacc tggcggacgt
     1441 ggccggcacg atcgccggcg acaacacgat cctggtcgtg cctcgggagg gggtggcggt
     1501 ccgctcggtg gaacgagcgc tccgcggcgt ccaggcgggt ccctgagcga tccgcggaca
     1561 cgacgagcaa cgagaggggc ccccggatgg gacgcgcggg ggcgggaagg agacggaacg
     1621 atgcagcgag cggtgctcgc ctactcgggg ggactcgaca cctccgtgac gatccggtgg
     1681 ctggccgagc agggctacga ggtgttcgcc gtcgcggtgg acgtgggtca gcgggaggac
     1741 ttcgcgctga tccgggagcg cggcgcgctg gccggggccg cccaggtccg ggtggtggac
     1801 gcggtggacc gcttcgccgc cgagttcctg acgccggccg tcaaggcgaa cggcctgtac
     1861 gaggacaagt acccgatggt ctccgctctg gctcggccct tgatcgtgga cgaggtcgtg
     1921 gcggtggccc gcgaggtcgg cgcgcaggtg gtggcccacg gttgcacggg caagggcaac
     1981 gaccaggtgc gcttcgaggt gtccttcggc gtgctggccc ccgacctgcg ggtcctggcc
     2041 ccgatccgcg acgacgggat cccgcgggag aaggccatcg ccctggccgg tgagtggggg
     2101 atcccgatcg ccgaggtggc cacctcctac tcgatcgacg agaacctctg gggacggacc
     2161 gccgagtgcg gtcccctgga ggacccgtgg gccgcgcctc cggaggacgc cttcgcccgc
     2221 acggccgcgc cgaaggatcg cccgggggaa ccgaccgaga cggtggtggc cttcgagcgc
     2281 ggggtgccgg tggcgctgga cggccggagc ctgcccctgc ccgagctgat ccggacggtc
     2341 gacgggctgg gcgggcggta cgggttcggg cgggtggaca tgatcgagaa ccggcgcgtg
     2401 ggcatcaagt cccgcgagct gtacgaggtg cccggggcgc tggcgctgat cggcgcccat
     2461 cgggccctgg aggacctgac gctggagcgc gaggtggccc acttcaagcc cgggatcgag
     2521 cagcggtggg cggacctcgt gtacgacggc ctgtggttct cgccgctccg gcgggcgctg
     2581 gacgccttcg tggatgccac gcagtcgcac gtgaccggcg aggtgcgcct gtcgttcgcg
     2641 ccgggttcgt gcacggtcgt cggtcgctgg tccgaagcat cgctgtacga cgtcggcctg
     2701 gccacgtacg gcccgcagga tgccttcgac caccggcacg cgcaggggtt cgtcagacta
     2761 tggggcatgc cgctcaaggt ctggtccgcc aggcagggga gcggatgagc ggcccctcgc
     2821 cccgcggcga ggaacggggg ggcccgtccg agcgagccct gtggggcggt cggttctcct
     2881 cgctcccctc gccggaggcc ttggagctcg gccggagcct gtccttcgac gtccggctcg
     2941 cggagcacga cgtccgcgcc ggggtcgccc acgtccgggg gctcgtggaa ggggggctcc
     3001 tggacgtggg ggaagccgag ctactgatcg gagctctgcg cgaggtcggc gccgagatcg
     3061 ccgccggccg gttcgcgttc cacccggcgg acgaggacgt gcactcggcc gtggagcggg
     3121 gggtgaccga gcgcctgggg gagatcggcg agaagctcca cgcgggtcgg agcc
//
LOCUS       DASQQQ010000264         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_159860, whole genome shotgun sequence.
ACCESSION   DASQQQ010000264 DASQQQ010000000
VERSION     DASQQQ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159860"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VE646_10005"
     CDS             <1..276
                     /locus_tag="VE646_10005"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZD80357.1"
                     /translation="EEAGAVMWYGWGSGSWVGWLLMTLVMIVFWGGLIVLVAWAVRSL
                     GRGAAPHRGDVPGDEAMRLLEQRFAKGEIDRDEFDERRTVLQGVRRS"
     gene            278..553
                     /locus_tag="VE646_10010"
     CDS             278..553
                     /locus_tag="VE646_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HZD80358.1"
                     /translation="MRGYSMDRDDLLGRLRRIEGQVRGVHRMVEQDQYCLDVLTQLNA
                     ITAAVRSVGLGLLDDHVHHCVRESLRRGEGEDKLEELIAAVARFAGR"
     gene            604..>2532
                     /locus_tag="VE646_10015"
     CDS             604..>2532
                     /locus_tag="VE646_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HZD80359.1"
                     /translation="MTGIEIAVTGAGVVLIGALAWFFFGPKEARRAEVRGGVQEVEIT
                     VKGGYSPNLIRVQQGVPLRLVFDRQENSDCSSRVVFPDFGVSKSLAPFGRTTVELRPD
                     RSGEFGFACGMNMLHGTLVVEASGGNGHGPAAGEGETAVGAATGSEAASPAPEGHTHE
                     TARAVGVGPSLEVGTTRRVELALRGSGVTCPTCVTNIEAALDGLPGVDRVQANFGAER
                     ITVDFDPNRVEVPDLTRRVAAAGYKVEERPEPGSEQTEDAEAAARRAEIRDLTWRVVV
                     GAILTAPVLFAVMVSDYLSPSWLPQILTNRWFELGMITPVMFYTGWPIHRTGWLTLRH
                     RTADMNTLVTVGSTAAFLYSLAVTATPGFFPQNVRSVYFEAVGTILTLILLGRLFEAR
                     AKAGTGEALRALIGLQAKTARVLRDGEEIEVPVDQVQLDDIVSVRPGEKVPVDGELTE
                     GQSAVDESMVTGEPIAVEKRPGDAVIGATINQTGAFRFRATKVGKDTMLAQIVKLVEQ
                     AQASKAPIQRLADLVASYFVPAVIFIALGTFVLWFDLHAVSALTFALVAAVSVLIIAC
                     PCALGLATPLSIMVATGKGAQRGILIRSAQALETAHKLDAIVLDKTGTITKGEPSLTD
                     VVPADGLDEGEFLRLVASA"
BASE COUNT          372 a          791 c          987 g          382 t
ORIGIN      
        1 gaggaggcgg gtgcggtgat gtggtacgga tgggggtcgg ggagctgggt cggatggttg
       61 ctcatgaccc tggtcatgat cgtcttctgg ggagggctga tcgtcctggt ggcgtgggcc
      121 gtccgatccc tcggtcgggg agcggctccg catcgagggg acgtcccggg cgacgaggcg
      181 atgcggctgc tggagcagcg gttcgccaaa ggcgagatcg accgcgatga gttcgacgag
      241 cggcgcaccg tcctgcaggg cgtcaggagg agctgagatg cggggctact ccatggaccg
      301 cgacgacctg ctgggacgtc tgcgccggat cgaggggcag gtccgtgggg tgcacaggat
      361 ggtggaacag gaccagtact gcctggacgt gctgacccag ttgaacgcga tcaccgcggc
      421 cgtgaggtcg gtgggcctgg ggctgctgga cgaccacgtc catcactgcg tccgggagag
      481 cctgcggcgg ggcgagggtg aggacaagct cgaggaactg atcgccgcgg tggcccggtt
      541 cgcggggcgc tgaaccaggc aacgaggacg gcggatggag cagatcagag ggaagtggtt
      601 ggcatgacgg ggatcgagat cgcggtcacc ggggccgggg tggtgctcat cggggcgctc
      661 gcctggttct tcttcggccc caaggaggcc cgccgggccg aggtccgggg tggcgtccag
      721 gaggtggaga tcaccgtgaa gggcggctac tcgcccaacc tgatccgcgt ccagcagggg
      781 gtgccgctgc gactggtgtt cgaccggcag gagaacagcg actgctcctc ccgggtggtg
      841 ttcccggact tcggggtgag caagtcgctg gctcccttcg gccgcaccac cgtggagctc
      901 cgccccgacc gcagcggcga gttcggcttc gcctgcggga tgaacatgct gcacgggacc
      961 ctggtggtgg aggcgtccgg tggcaacggg cacggtcccg ctgccggtga gggagagacg
     1021 gccgtggggg cggccacggg gtcggaggcg gcgagccccg cccctgaggg acacacgcac
     1081 gagacggcca gggcggtggg cgtaggtccc agcctcgagg tcggcacgac cagacgggtg
     1141 gagctcgcgc tgcgggggag cggggtgacc tgcccgacgt gcgtgaccaa catcgaggcg
     1201 gcgctggacg gtctgccggg ggtggaccgg gtgcaggcca acttcggggc cgagcggatc
     1261 accgtggact tcgatccgaa ccgggtcgag gtgccggacc tgacgcggag ggtcgccgcc
     1321 gccgggtaca aggtcgagga gcggcccgag cccggatcgg agcagaccga ggatgccgag
     1381 gccgccgccc gccgggccga gatccgggat ctcacgtggc gggtcgtggt cggggccatc
     1441 ctgaccgctc cggtcctgtt cgcggtcatg gtgagcgact acctgagccc gtcgtggctg
     1501 ccgcagatcc tgaccaaccg ctggttcgag ctcgggatga tcacgccggt catgttctac
     1561 acgggctggc cgatccaccg gaccggctgg ctgacccttc gccaccgtac ggccgatatg
     1621 aacaccctgg tcacggtggg gtccacggcg gcgttcctct acagcctcgc cgtgaccgcg
     1681 accccgggct tcttccccca gaacgtccgc tcggtctact tcgaggcggt gggcacgatc
     1741 ctgaccctga tcctgctggg acggctgttc gaggcccggg ccaaggcggg gaccggcgag
     1801 gcgctccggg ccctgatcgg cctgcaggcc aagaccgccc gcgtgctccg cgatggggag
     1861 gagatcgagg tccccgtcga ccaggtgcag ctggacgaca tcgtctcggt gcggcccggg
     1921 gagaaggtcc cggtggacgg cgagctcacc gagggccagt ccgccgtgga cgagtccatg
     1981 gtgaccgggg agccgatcgc ggtggagaaa cgaccggggg acgccgtgat cggggccacc
     2041 atcaaccaga ccggggcctt ccgtttccgg gccaccaagg tgggcaagga cacgatgctc
     2101 gcccagatcg tgaagctggt ggagcaggcc caggcgtcca aggctcccat ccaacgcctg
     2161 gccgacctgg tggcgtccta cttcgtcccg gcggtgatct tcatcgccct ggggacgttc
     2221 gtgctgtggt tcgacctgca cgcggtctcg gcgctgacct tcgccctggt ggcggcggtc
     2281 tcggtgctga tcatcgcctg cccctgcgcc ctgggcctgg cgacaccgct gtcgatcatg
     2341 gtggccaccg gcaagggggc tcaacggggg atcctgatcc gctcggcgca ggcgctggag
     2401 accgcccaca agctggatgc gatcgtgctg gacaagacgg ggaccatcac caagggggag
     2461 ccctccctca ccgacgtggt gccggcggac ggcctcgatg agggcgagtt cctgcggctg
     2521 gtggcctcgg cc
//
LOCUS       DASQQQ010000265        11526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_316739, whole genome shotgun sequence.
ACCESSION   DASQQQ010000265 DASQQQ010000000
VERSION     DASQQQ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11526
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316739"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VE646_10020"
     CDS             <1..422
                     /locus_tag="VE646_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZD80360.1"
                     /translation="ELMPEGVHFFSMYTQWAPDDWNQEPHRDELEAYADRIFDIYNDL
                     APNFKASVIARQIIGPYDMEHDLGLIGGNIFHGELSVDQLFHMRPAPGYADYRTPIKG
                     LYHGSSATHAGGGVVGIPGWQAFRQAKRDRMLSKKST"
     gene            433..2559
                     /locus_tag="VE646_10025"
     CDS             433..2559
                     /locus_tag="VE646_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HZD80361.1"
                     /translation="MGGDAAPGDPRPALQAMLEARSVAVVGASVREGSVGHQSLVELR
                     KGRFAGEVFPVNPKYDQILGWRCSPSLSELDRPVDLAILAVSNARLEEQLRLAADTGV
                     RSAVIFASCYESPREGVPPLTERLGAIARGAGMAVCGGNCMGFANIEGGVRALGFYEP
                     EDLRPGPLAFISHSGSGFSALLHNERGLRFNLAVSSGQEFATTTADYLRYVMDLESTR
                     AVGLFIETARDPAAFTQALRTAAERDVPVVVLKVGREALTRRMVTAHSGALAGEDGAY
                     EALFEANGVLRVESLDEMADTLALFAGGRRAAPGGLASVHDSGGERALLVDSAASVHV
                     PLARISEATTRRMADLLEEGLLPVNPLDFWGTGRDANEVITGCMRALLDDPDTGALAF
                     TVDLTTEDSPDMGYIGMALQVYPETDKPVAMLSNLSSGIDRSDVRHLDEAGIPVLEGT
                     LTGLAAFGHLFEYRDHRALPPVDPSSPVPGEVRERWRARLSSGEAFDEREGLAMLADF
                     GIPVVPHEGVGSSEEAVVAAEGLGFPVVLKTASPGVQHKSDVGGVRLGLADAEAVTDA
                     YRDISGRLGPSVTVARIAPPGVEVALGVVRDPQFGPLVLVAAGGVLVELLKDRRLAMP
                     PLDPARAHRLIDGLAMRPLFDGVRGSPACDVDALARTVVALSWLAHDLGEHLEALDVN
                     PVICGPDGCLAVDSLVVPRHGSVVQR"
     gene            complement(2593..4038)
                     /locus_tag="VE646_10030"
     CDS             complement(2593..4038)
                     /locus_tag="VE646_10030"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZD80362.1"
                     /translation="MPDPVHHREHPRDPAHPLSRSRRDFLRTSGLALAGATLYACTGG
                     DRKVPVPSTGATDTPSTITTRWPVKRVLYVMLENRSFNNIFGRFPGVKSSLTGVSYGT
                     ETPLHPCPDWLPGDLPHDRAAALNCLNGGSLDGFATGIYGAYFAYTVVQERQIPNYWT
                     WAREYALSDHFFASALGPSYPNHFYFIAGQANGVIDNPENIGTLPPKQKGQYAFKSWG
                     CDAVGDDVFVFAKDEHGNLTKHDTCFTFRTVGEQLSEGGVDWAYYAAVPYQVGYFWNA
                     YNGIHQVFHDRAYWNAHMRPVDSLVKDIEGGRLPAVTWVTPLFQLSDHPPASSSGAHN
                     WVSDVVNAVMRSDLWKDTVIFITWDEWGGFYDPVKPPVVDDVGLGFRVPLLTIGPHVR
                     RGVIDDEVGEFSTPLRFIADNWGLDHLTPRIANTHNFEHVFDFSKPPRPPVFATKKVK
                     TYGGDPLDWPFDTGGTQPLWPAGTEPVTRPW"
     gene            4091..4786
                     /locus_tag="VE646_10035"
     CDS             4091..4786
                     /locus_tag="VE646_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80363.1"
                     /translation="MSLANLVTMRRLALLLVVAVLAPGCGGGSGDAARSPSLTQAPPV
                     SPLASGGSAPPATVTSPATPATPPPTSGAVTSSCVHGWITPPSGSPRFLQPLGVIRRT
                     TGVEGPLVVVDLRYFEGPESPPSEQGYLQVVQRWYVKLYAKRDPAFQGRFLVEARRFG
                     RGLSAVAPYDTHGFRSPDWVGFQYDAADPTSKTYPGLPGRWAGVPYDFVTGGGGLTFP
                     GLPREVVGCLAGT"
     gene            complement(4788..6194)
                     /locus_tag="VE646_10040"
     CDS             complement(4788..6194)
                     /locus_tag="VE646_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HZD80364.1"
                     /translation="MADAISEAIPSGALDPTASTARPGARVRVGVVLAAGRSERLSGV
                     TGGGSKALVRVGGLALVERAVRTLLAAGLERVVVVVGYQAGPVAAVVDRLAPGRVRAA
                     FAERWEAGNGASLAAAEPLVGEEELFALITADHVFAEDALEPLFGVDRPAVLVDGAPE
                     PAAWAEGTRVRLQAGRAVAFSKQLGDPSIDCGAFVLPPAVFDAQRRAASRGDASLAGA
                     VSELAGATPLFAVSLPPGAWWQDVDTPEDLRRVRHRLRRSLIKDADGPVSRYLNRPVS
                     TRLSMALGPVPLHPDVVSVIACLVGVAGGWILGLGLGVLGAILVHASSVLDGVDGEVA
                     RLQVRTSAGGALLDGVLDRLADTAVLAGLAAWAIQAGWAPVPVAAVGVVATAGAMLSM
                     ASKDRIAALGIPPTPERRIGYLLGGRDARLLFAALTGVAGRPLWGLIAIAATSALSLS
                     VRLLLARSTLRGAWAGPR"
     gene            complement(6261..7436)
                     /locus_tag="VE646_10045"
     CDS             complement(6261..7436)
                     /locus_tag="VE646_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZD80365.1"
                     /translation="MDLQLEPEDRGLKERARAFTEEVLFPLELECEERGGLSSESWAR
                     AKGAVSAGGWAAINHAVDDGGQGLDLFSQILVEEEWGKATGALWDIPWRPAIPLAMAT
                     DAQRERYLRPACRGERRDAYAITEAGAGSDPTMVETTAVRDGDGWRLDGTKWHVTSGD
                     VADFFLVHAHVDGDPAKATIFLVDKELPGVKLVRTPRYMHTFVFEHPIFAFEGVHVGP
                     EQVLGEVGQGFELTKDWFVEERLMIGARTMGAGTRALDLSLAFAQGRVQFGRPIVEHQ
                     AVGFMLADMAAELMAAKAMLYRVGWEAARGGIDRKRLHALASAVKLVCSETAGRVVDR
                     AVQIHGGRGYMREQPVERLYREIRVDRIWEGTSEIQRSVIQNELRKRGGGAYTGWPS"
     gene            7524..8735
                     /locus_tag="VE646_10050"
     CDS             7524..8735
                     /locus_tag="VE646_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZD80366.1"
                     /translation="MSRTVFRGGRIFDGTGAPIEPADLVVQDGRIVDVGPDLDGDAAV
                     DVTGRAILPGLFDCHAHLAMRYEDLDELAVAHQPFSYRFFTVAENLRRTLALGITTVR
                     DASGADLGMKMAVEDGLLVGPRMQLSVTMLSMTGGHNDPWLPSGATSPWPVYPGMPDG
                     VCDGLDGVSAKVREVIRAGADVIKIASSGGFLSPLDDPSLPHFSQGEIEAMVRTAADM
                     GRWVMAHAHGAEGIKRAVRAGVRSVEHGTFLDDEGISLMLQSGTWLVPTLTAGDTTDE
                     LANDPKVPEPVRQKLRGLGRPELEAFQRAAQAGVKVAMGTDCPVAPHGTNLRELQLMT
                     ENGYTPTQALLAATSGAAELMGLRDELGTIEPGKRADLVVVEGDPFDFATLPGHIRQV
                     WKDGVRVAGEG"
     gene            complement(8800..9963)
                     /locus_tag="VE646_10055"
     CDS             complement(8800..9963)
                     /locus_tag="VE646_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80367.1"
                     /translation="MDPVQRRAVRNGIVAFAVTALIGFGINALVIRGDEAAGRRASST
                     GPPSASPTCTSTWEPVDTVDPDRSGSQLVAVTALSPSDVWAVGSTGDPQEPSNTLVEH
                     WDGNRWRRVDTPNGGTMGNALVAVDGTGRKDAWAVGSASDGAGYQPLILHWDGSTWET
                     ALAPNLLGGAELSGVIALSPTDAWAVGHRGDPTVGSDRALVLHWNGSNWTEAQIEASG
                     GRSSLVAVDGTAADDLWAVGYHQKRSAIMHGDGKRWRWVDLKPRGRLLDVTVAAPDQV
                     WAVGSSILRWNGSRWKPGGTIGSTAELNAVSEPSTAGLWAVGDRVAGKKTRAIVRRFD
                     GTRWSSVEGSGVPGSESLGGVSALPDGVAWGVGYRETKGGRRTLAARMVLTCR"
     gene            10079..>11526
                     /locus_tag="VE646_10060"
     CDS             10079..>11526
                     /locus_tag="VE646_10060"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZD80368.1"
                     /translation="MAAVRDAVIVGAGHNGLACAAYLARTGMDVLVLERRDVLGGAAA
                     TEEPWPGYRIWSASYVVSLMPQRIVDELGLKRFGYEVSIITPDYFVPFPDGTSLTLWG
                     DTQRDAENIARFSKHDAEAYVRFDRYFDRVARLLKDLLFVVPPNPRLGELPRWIRTAA
                     RLRGWTGRDIHETIRLFTMSAADFLDEWFEEERVKGAMATQAIIGAWCGPMTPGSAYV
                     LMHHWIGEIDGHVGAWGWVKGGMGGVSTALAGVAQAAGAEIRTQAEVRRVATSHGGRA
                     VGVELADGSSIRARRVVSDAHPITTYLDLDGEERLPDDVVADVRRFRTRSGSVKVNVA
                     LSELPAFPGWDQEGDVHRGLAAVSPSIEYLERAWDDAKYGRMSQHPYVEVVFPTAHEP
                     NGLAPEGKHIMLAFTQYGPYEFRDGSWDEGAREEWGRRVIGTLGEFAPTLPDAVEQIE
                     VLTPRDIETRFGLLGGNIMQGELTPDQLFSFRS"
BASE COUNT         1617 a         4126 c         4110 g         1673 t
ORIGIN      
        1 aggagctgat gcccgaaggc gtgcacttct tctcgatgta cacgcagtgg gcgccggacg
       61 actggaacca ggagccccac cgcgacgagc tcgaggccta cgcggaccgg atcttcgaca
      121 tctacaacga cctggcgccg aacttcaagg cgtcggtgat cgcccggcag atcatcgggc
      181 cctacgatat ggagcacgac ctggggctga tcggcggcaa catcttccac ggcgagttgt
      241 ccgtggacca gttgttccac atgcggccgg cccccggcta cgcggactac cggaccccga
      301 tcaagggcct gtaccacggc tcgtccgcca cccacgcggg cggcggggtc gtgggcatcc
      361 ctgggtggca ggcgttccgg caggccaagc gggaccgcat gctctcgaag aagagcacgt
      421 agcgttcgga tcgtgggagg cgacgccgcg ccgggggacc cgcgccccgc cctccaggcc
      481 atgctcgagg cgcggtccgt ggccgtggtg ggcgcgagcg tccgcgaggg ctcggtgggc
      541 catcagagcc tggtcgagct ccgcaagggc cggttcgcgg gcgaggtctt cccggtcaac
      601 ccgaagtacg atcagatcct cggctggcgg tgctcgccct cgctgtccga gctcgaccgc
      661 ccggtggacc tagcgatcct ggcggtgtcg aacgcgcgtc tggaggagca gctgcggctc
      721 gccgccgaca ccggggtccg ctcggccgtg atcttcgcca gctgctacga gtccccacgc
      781 gagggcgttc cgcccctgac cgagcggctg ggggcgatcg cgcgcggggc cgggatggcc
      841 gtctgcggcg gcaactgcat gggcttcgcc aacatcgagg gtggggtccg ggcgctgggg
      901 ttctacgagc cggaggatct gcggcccggg cccctcgcct tcatcagcca ctcgggatcg
      961 ggcttctccg cgctgctgca caacgagcgt gggctgcgct tcaacctggc ggtgtcatcg
     1021 ggtcaggagt tcgcgaccac caccgccgac tacctccggt acgtcatgga cctggagagc
     1081 acccgagcgg tcggcctgtt catcgagacc gcccgcgacc cggcggcctt cacccaggcg
     1141 ctccgcaccg cagccgagcg agacgtgccc gtcgtggtcc tgaaggtcgg ccgcgaggct
     1201 ctcacgagga ggatggtgac ggcgcactcg ggcgcgttgg ccggcgagga cggggcctac
     1261 gaggccctgt tcgaggcgaa cggcgtcctt cgggtggaga gcctggacga gatggccgac
     1321 accctggcct tgttcgccgg tggacgtcga gcggccccgg gcgggctggc cagcgtgcac
     1381 gactcggggg gcgagcgggc cttgctggtg gactcggccg catcggtcca cgtaccgctg
     1441 gcccggatct cggaggcgac cacgcggcgt atggccgatc tcctggagga gggtctgctg
     1501 ccggtgaacc cgctggactt ctgggggacc gggcgcgatg cgaacgaggt gatcaccggc
     1561 tgcatgcgcg ccctgctgga cgatcccgat acgggtgccc tggccttcac cgtcgacctc
     1621 accaccgagg acagcccaga catggggtac atcgggatgg cgctgcaggt gtacccggag
     1681 acggacaagc cggtggccat gctctcgaac ctatccagcg ggatcgaccg cagcgacgtc
     1741 cggcacctgg acgaggccgg gatcccggtg ctggagggaa cgctcaccgg cctggccgcg
     1801 ttcgggcacc tgttcgagta ccgggaccac cgcgcgctcc cgccggtcga cccgagctca
     1861 cccgtgcccg gggaggtccg cgagcgctgg cgggctcggc tgtcctccgg agaagcgttc
     1921 gacgagcgcg aggggctggc gatgctggcc gacttcggta tccccgtggt gccgcacgag
     1981 ggagtgggga gctcggagga ggcggtcgtc gcggccgagg ggttgggctt cccggtcgtc
     2041 ctgaagacgg cctcaccggg ggtccagcac aagtcggacg tgggcggggt gcggctggga
     2101 ttggccgacg ccgaagccgt gaccgacgcg taccgggaca tctcgggccg gctgggacct
     2161 tcggtcaccg tggccaggat agcgccgccc ggtgtggagg tggccttggg tgtggtccgc
     2221 gatccgcagt tcgggccgct ggtcctggtg gcggccggcg gggtgctggt cgagctgttg
     2281 aaggatcggc gcctggcgat gccgcccctg gacccggctc gggcccatcg gctgatcgac
     2341 ggtctcgcga tgcgccccct gttcgacggg gtacgcggat cgccggcctg cgacgtggat
     2401 gctctggccc gcacggtggt cgcgctgtcc tggctggcgc acgacctcgg agagcacctc
     2461 gaggcactcg acgtgaaccc cgtgatctgc ggacccgacg gctgcctggc cgtggattcc
     2521 ctggtcgtgc cccgacacgg gtccgtcgtg cagcgctgat ccccgcgccg tcgacgtgtg
     2581 gccggcctcc ggctaccagg gcctggtgac cggctcggtc ccggccggcc acaggggctg
     2641 ggtgccgccg gtgtcgaagg gccagtccag ggggtccccg ccgtaggtct tcaccttctt
     2701 ggtggcgaag accggggggc ggggcggctt ggagaagtcg aagacgtgct cgaagttgtg
     2761 ggtgttggcg atgcgcggcg tcaggtggtc caggccccag ttgtccgcga tgaagcggag
     2821 cggcgtagag aactcgccga cctcgtcgtc gatcaccccc cgacgcacgt ggggaccgat
     2881 cgtcagcaac ggcacgcgga agcccaggcc gacgtcgtcc acgacgggcg gcttcaccgg
     2941 gtcgtagaaa cccccccact cgtcccacgt gatgaagatc accgtgtcct tccacagatc
     3001 gcttcgcatc acggcgttca ccacgtcgct cacccagtta tgcgctcccg aggagctggc
     3061 cggtgggtga tcggacagct ggaacaacgg ggttacccag gtgaccgccg gcagccttcc
     3121 cccctcgatg tccttcacca gcgagtccac cggacgcata tgcgcgttcc agtacgcgcg
     3181 gtcgtggaag acctggtgga tcccgttgta cgcgttccag aagtagccca cctggtacgg
     3241 gacggccgcg tagtacgccc aatcgacccc gccctccgag agctgctcgc ccaccgtcct
     3301 gaacgtgaaa caggtgtcgt gcttcgtgag gttcccgtgc tcgtccttcg cgaacacgaa
     3361 cacgtcgtcg ccgacggcat cgcagcccca gctcttgaac gcgtactgtc ccttctgctt
     3421 gggcggcagg gtcccgatgt tctccgggtt gtcgatcacg ccgttggcct gcccggcgat
     3481 gaagtagaag tggttcgggt acgagggccc gagcgccgag gcgaagaagt gatccgagag
     3541 cgcgtactcg cgggcccacg tccagtagtt ggggatctgg cgctcctgca ccaccgtgta
     3601 cgcgaagtag gctccgtaga tccccgtcgc gaacccgtcg agggagccgc cgttcaggca
     3661 gttcagggcg gccgctcgat cgtgcgggag gtcgccgggc agccagtccg ggcatggatg
     3721 cagcggtgtc tcggtgccgt aggagacacc ggtgagcgag gacttcacgc ccgggaagcg
     3781 tccgaagatg ttgttgaagc tccggttttc cagcatcacg tagagcaccc gcttcaccgg
     3841 ccagcgtgtg gtgatggtgg agggcgtgtc ggtcgctccg gtgctgggga ccggtacctt
     3901 ccggtccccg ccggtgcagg cgtacagggt ggcgcctgcc agcgcgagcc ccgaggtgcg
     3961 caggaagtcg cgccgactcc gggagagggg gtgagcgggg tcccgcgggt gttcgcgatg
     4021 gtgcacgggg tcgggcatgc ggcccacatt cctaccagac gcggcgccgg acggtatcct
     4081 cgcggggccc gtgtctctcg cgaacctggt gacgatgcga aggctggcgc tgcttctggt
     4141 ggtcgccgtg ctcgcgcccg ggtgcggggg tggctccgga gacgccgccc gctcgccctc
     4201 gctgacccag gcgcccccgg tctcaccgct cgcgtccggc ggctccgctc cacccgcgac
     4261 ggtcacctcg ccggccaccc cggccacgcc tccgcccacc tcgggcgccg tgacgtcgag
     4321 ctgcgtgcac ggatggatca cccccccgtc cgggagccct cggttcctgc aaccgctcgg
     4381 cgtgatccgg cggaccacgg gggtcgaggg tccgctggtg gtggtggacc tgcgctactt
     4441 cgagggaccc gagtccccgc catccgagca gggctacctg caggtcgtcc agcgctggta
     4501 cgtgaagctc tacgcgaagc gggatccggc cttccagggt cggttcctgg tggaggcccg
     4561 ccgattcggc cgagggctgt ccgccgtagc cccctacgac acgcacgggt tccgctcgcc
     4621 ggactgggtc gggttccagt acgacgccgc cgaccccaca tcgaagacct atcccggtct
     4681 gccggggcgg tgggctgggg tcccgtacga cttcgtcacg gggggtgggg gcctcacgtt
     4741 cccggggctg cccagggagg tcgtcggctg cctggccggg acctgagcta ccgcggtccg
     4801 gcccaggccc cccgcagcgt ggaacgcgcc agcagcagcc ggacggacag gctgagcgcg
     4861 gaggtcgcgg cgatcgcgat caggccccac agcggacggc ccgctacccc ggtgagcgcc
     4921 gcgaacagca gccgcgcgtc ccgccctccg agcaggtacc cgatccgtcg ctccggcgta
     4981 ggagggatcc ccaaggcggc gatccggtcc ttgctggcca tcgacagcat cgctcccgcg
     5041 gtggcgacca ccccgaccgc cgccaccggc accggcgccc agccggcctg gatggcccac
     5101 gcggcgagcc cggccaggac cgccgtgtcc gccaggcggt ccagcacgcc gtccagcagc
     5161 gcgccgcccg ccgaggtccg gacctgaagg cgcgccacct cgccgtccac cccgtccagc
     5221 accgaggagg cgtgcacgag gatcgccccc agcacaccga gcccgaggcc caggatccag
     5281 ccacccgcca cgcccaccag gcacgcgatc acggacacca cgtccggatg gagcggtacg
     5341 ggaccgagcg ccatggacag tcgcgtggac accggccggt tcaggtagcg ggagacggga
     5401 ccgtcggcgt ccttgatcag ggaccggcgg agccggtgcc gcacccggcg gaggtcttcg
     5461 ggcgtgtcga cgtcctgcca ccaggccccc gggggcaggg agacggcgaa gaggggcgtc
     5521 gcgccggcca gctcggacac cgcgccggcg agcgaggcgt caccccgaga cgctgcccgc
     5581 cgctgcgcgt cgaacaccgc cgggggaagc acgaacgctc cgcagtcgat cgaggggtcg
     5641 ccgagctgct tggagaaggc cacggcgcgc ccggcctgca ggcggacccg ggtgccctcc
     5701 gcccacgctg cgggctcggg agcgccgtcc acgaggaccg cgggccggtc gaccccgaac
     5761 agcggctcga gcgcgtcctc ggcgaacacg tgatcggcgg ttatgagcgc gaacagctcc
     5821 tcctccccga ccaagggctc cgcggcggcc agggaggccc cgtttccagc ctcccaccgt
     5881 tcggcgaagg cggctcgaac ccgtccgggt gcgagccggt cgaccacggc ggccaccgga
     5941 ccggcctggt agcccacgac caccaccacc cgctccaggc cggcggccag gagcgtcctg
     6001 accgcgcgtt ccaccagggc cagccccccc acccgaacca gggccttgga gcccccaccg
     6061 gtcacgcccg acaggcgctc ggatcgaccg gcggcgagga ccacccccac ccggacccgc
     6121 gcgcccggac gagcggtcga tgccgtggga tcgagcgctc cggatgggat cgcctctgag
     6181 atggcgtcgg ccacagctcc tcctgtgaac gggacgtccc taggttccac cacccgccgg
     6241 gcgcgggcaa gccgccgacg tcagctcggc cagcccgtgt acgcgccgcc gccccgcttc
     6301 cgcagctcgt tctggattac cgagcgctgg atctccgagg tgccctccca gatccggtcc
     6361 acgcggatct cccggtacag gcgctccacc ggctgctccc gcatgtaccc gcgcccgccg
     6421 tggatctgga cggcgcggtc caccacccgc cccgcggtct ccgagcacac gagcttgacg
     6481 gccgaggcca gggcgtggag ccgcttgcga tcgatcccgc cccgggccgc ctcccagccc
     6541 acgcgataga gcatcgcctt ggccgccatc agctccgccg ccatatcggc cagcatgaac
     6601 ccgaccgcct ggtgctccac gatcggccgt ccgaactgga cccgcccctg ggcgaacgcg
     6661 agcgacaggt ccagcgctcg agtacccgcg cccatcgtcc gggcgccgat catcagtcgt
     6721 tcctccacga accagtcctt ggtgagctcg aagccctggc ctacctcgcc caggacctgc
     6781 tccgggccca cgtggacccc ctcgaaggcg aagatggggt gctcgaacac gaaggtgtgc
     6841 atgtaccggg gggtccgcac cagcttcacc cccggcagct ccttgtccac caggaagatc
     6901 gtggccttcg ccggatcgcc gtcgacgtgg gcgtgcacca ggaagaagtc cgccacgtcg
     6961 ccggaggtga cgtgccactt cgtcccatcg agccgccagc cgtcgccatc gcgcaccgcg
     7021 gtggtctcga ccatcgtggg gtccgacccc gcccccgcct cggtgatcgc gtaggcgtcc
     7081 cgacgctcgc cccggcacgc cgggcgcagg taccgctccc gctgggcgtc ggtggccatc
     7141 gcgagcggga tggcgggtct ccaggggatg tcccacaggg cgccggtggc cttgccccat
     7201 tcctcctcca ccaggatctg cgagaacaga tccagcccct gcccgccgtc gtcgacggca
     7261 tggttgatcg cggcccatcc gcccgcgctc acggccccct tcgccctggc ccagctctcc
     7321 gaggacagac cgccgcgctc ctcgcattcg agctccagcg ggaacaggac ctcctcggtg
     7381 aaggcccgtg cccgctcctt cagcccccgg tcttccggct ccagttgcag gtccacggcg
     7441 aacgtcctcc tctggccgaa cggcggtcca gcgcaaagtg tagggtcccc gcgcatggga
     7501 ccggagggaa cggaggtcgg acggtgagca ggacggtgtt ccggggcggt cggatcttcg
     7561 acgggaccgg cgctcccatc gagccggccg atctggtggt gcaggacggg cggatcgtgg
     7621 acgtgggtcc cgatctggat ggcgatgcgg cggtggacgt gacggggcgg gcgatcctgc
     7681 ccgggctgtt cgactgccac gcgcacctgg ccatgcgcta cgaggatctc gacgagctcg
     7741 cggtggcgca ccagcccttc tcctaccgct tcttcacggt ggccgagaac ctgcgccgga
     7801 ccctcgctct ggggatcacg acggtgcggg atgcttcggg ggccgatctc ggcatgaaga
     7861 tggcggtcga ggatggtctg ctggtcggtc cgaggatgca gctctcggtc accatgctgt
     7921 cgatgaccgg agggcacaac gatccctggc tgccgagcgg cgccacctct ccctggcccg
     7981 tgtatccggg gatgcccgac ggggtctgcg acggcctgga cggggtcagc gcgaaggtcc
     8041 gggaggtgat ccgcgccggc gccgacgtga tcaagatcgc ctcgagcggt gggttcctct
     8101 cccctctgga cgatccgagc ttgccgcact tctcccaggg cgagatcgag gcgatggtgc
     8161 ggaccgccgc ggacatgggc cgctgggtga tggcccacgc gcacggcgcc gagggcatca
     8221 agcgggcggt gcgggccggg gtccggtccg tggagcacgg gacgttcctg gacgacgagg
     8281 gcatctcgct gatgctccag agcggcacct ggctggtgcc cacgctcacg gccggggata
     8341 ccaccgacga gctggcgaac gacccgaagg tccccgagcc ggtgcgccag aaactccgcg
     8401 ggctggggcg accggagctg gaggcgttcc agcgggcggc gcaggccggg gtcaaggtgg
     8461 ccatgggtac cgactgcccg gtggcgccgc acggcacgaa cctgcgcgag ctccagctca
     8521 tgaccgagaa cggctacacc ccgacgcagg cactgctggc ggcgacctcc ggggccgcgg
     8581 agctgatggg cctccgggac gagctcggca cgatcgagcc cggcaagcgc gcggacctcg
     8641 tggtggtgga gggcgacccg ttcgacttcg ccacgctgcc cgggcacatc cgtcaggtct
     8701 ggaaggacgg cgtgcgggtg gcgggtgagg gatgagcgag atcaccgccc acccgccggc
     8761 gtgacgcggt cggtccctgt ccttccgtgg tgagggcact cagcgacagg tgaggaccat
     8821 gcgggcggcc agtgtccgcc ggcctccctt cgtctcccgg tagccgaccc cccaggccac
     8881 cccgtcgggg agcgcggaga cgccccccag gctctccgag ccggggacac cggatccctc
     8941 caccgaggac catcgcgtgc cgtcgaagcg ccggacgatg gctcgggtct tcttgcctgc
     9001 cacgcgatcg cccaccgccc acaggccggc ggtggacggc tcgctcaccg cgttcagctc
     9061 ggccgtggac ccgatcgtgc cacccggctt ccagcgggag ccgttccagc gcaggatgga
     9121 cgatcccacg gcccacacct gatcgggagc ggcgaccgtc acgtccagca accgccctcg
     9181 gggcttcagg tccacccaac gccatcgctt gccgtcgccg tgcatgatcg cggaccgctt
     9241 ctggtggtag ccgaccgccc acaggtcgtc ggcagccgtc ccgtcgaccg ccacgagcga
     9301 gctgcggcca ccgctggcct cgatctgggc ctcggtccag ttggagccgt tccagtgcag
     9361 caccagcgcg cgatccgagc cgaccgtcgg gtcgccccgg tggcccacgg cccaggcatc
     9421 ggtcggggag agggcgatca cgcccgagag ctcggcgccg ccgagcaggt tcggcgccag
     9481 tgccgtctcc cacgtggacc cgtcccagtg caggatcagg ggctggtacc ccgcaccgtc
     9541 ggaggcggag cccacggccc aggcgtcctt ccgcccggtc ccgtccacgg ccaccagcgc
     9601 gttgcccatc gtgccgccgt tcggggtgtc cacccgccgc cacctgttgc cgtcccagtg
     9661 ctccaccaac gtgttggacg gctcctgcgg gtcacccgtg gagcccaccg cccacacgtc
     9721 gctcgggctc agagcggtga ccgccacgag ctggctgccg gaccgatccg ggtccaccgt
     9781 gtccaccggc tcccaggtgg aggtgcaggt gggcgaggcg ctggggggcc ctgttgagct
     9841 ggcccgccgg ccggcggcct cgtccccccg gatcaccagg gcgttgatgc cgaagccgat
     9901 cagtgctgtg acggcgaagg ccacgatgcc gttgcggacc gcccgtcgct ggaccgggtc
     9961 catggtcacc aacgctactt cgaacccggc cccggcgccc ggtctccggg cccgcccatg
    10021 agccgcgagg cgcgtccggg gttggcgggg ccggacgcct ccgctacgct ccgcccggat
    10081 ggccgccgtg cgagacgccg tgatcgtggg ggccggtcac aacggcctgg cgtgtgcggc
    10141 ttacctggct cggacgggga tggacgtgct ggtgctcgag cgccgggacg tcttgggcgg
    10201 agcggcggcc accgaagagc cctggcccgg ctaccggatc tggagcgcct cctacgtggt
    10261 ctcgctgatg ccgcaacgga tcgttgacga gctggggctc aagcggttcg ggtacgaggt
    10321 ctcgatcatc acccccgatt acttcgtccc gttccccgac ggaacctccc tcaccctgtg
    10381 gggcgacacc cagcgcgacg ccgagaacat cgcgcggttc tcgaagcacg atgcggaggc
    10441 ctacgtgcgg ttcgatcggt acttcgatcg ggtggcccgt ctgctcaagg acctcctgtt
    10501 cgtggtcccc ccgaaccccc ggctcggcga gctcccccgg tggatccgca ccgcggccag
    10561 gctccggggg tggaccggcc gcgacatcca cgagaccatc cggctgttca cgatgagcgc
    10621 cgccgacttc ctcgacgagt ggttcgagga ggagcgggtg aagggcgcga tggccaccca
    10681 ggccatcatc ggcgcctggt gcggaccgat gaccccgggg tccgcctacg tgctgatgca
    10741 ccactggatc ggcgagatcg atggtcatgt cggcgcgtgg ggctgggtca aggggggcat
    10801 gggcggggtc tcgaccgcgc tggctggagt cgcgcaggcc gccggggccg agatccgcac
    10861 ccaggccgag gtccgacggg tggccacgag ccacggcggg cgggccgtgg gcgtggagct
    10921 cgcggacggc tcctcgatcc gggcgcgccg ggtggtttcg gacgcccatc ccatcaccac
    10981 gtacctcgac ctggacggcg aggagcggct gcctgacgac gtggtcgccg acgtacgccg
    11041 gttccggacc cgttcgggtt cggtcaaggt gaacgtggcg ttgtccgagc tccccgcgtt
    11101 ccccggttgg gaccaggagg gcgacgtcca tcgcggcctg gccgcggtct cgccctcgat
    11161 cgagtacctg gagcgggcct gggacgacgc gaagtacggc cggatgtccc agcacccgta
    11221 cgtggaggtc gtcttcccca ccgcgcacga accgaacggc ctggcccccg agggcaagca
    11281 catcatgctc gccttcaccc agtacggacc ctacgagttc cgcgatggct cgtgggacga
    11341 gggcgcgcgt gaggagtggg gtcgtcgggt catcggcacg ctgggcgagt tcgcccccac
    11401 gctgcccgac gccgtggagc agatcgaggt gctgacccct cgggacatcg agacccggtt
    11461 cggcctgctc ggcgggaaca tcatgcaggg cgagctcacg cccgaccagt tgttcagctt
    11521 ccgttc
//
LOCUS       DASQQQ010000266         3978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_316859, whole genome shotgun sequence.
ACCESSION   DASQQQ010000266 DASQQQ010000000
VERSION     DASQQQ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316859"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /locus_tag="VE646_10065"
     CDS             <1..1230
                     /locus_tag="VE646_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZD80369.1"
                     /translation="SVLATGQVIRGSAPVRELVQRCRAVPLPPEPEPSALRPPMSFPG
                     GAGNVGRGEGLRRGVGFALGYKNIAYSEGFDDFHQARVTLSRGPSGPLAEVHTAGVEC
                     GQGFSTVVTQVARTELGIDTIVLHAADTEVDSAGSTSASRQTFMGGGAVRLASRAVRE
                     ELFDRAREGLGVGGELSVAEGRILTDGVAVADVVDLLDQPVAATRTFHHRPTEPLDAK
                     GQGDVHVLFAFGALRAVVEVDEELGLVRVVQLASALDVGRAVNPQGVEGQSEGGAAQG
                     LGLALMEELQTREGVIANASFTDYLIPTTLDMPSVVTAIVEDPEPEVPYGAKGVGEIS
                     TIVSTAAIVAAIRNATGRVLNRAPVRPDQIAGLEGPVPARPWPPVPEVPSQEPVPATF
                     GVPLGQSGLSERDATRR"
     gene            1254..2243
                     /locus_tag="VE646_10070"
     CDS             1254..2243
                     /locus_tag="VE646_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZD80370.1"
                     /translation="MVERASLSGRDFIETLDWTTGEIDEVEWLATELKRKFRSGEPHR
                     LLPDKTLFMLFLDKSTRTRNAFEAGMTQLGGHAHFLDAEKTQVAHGESPKDMGIILSR
                     YGHGLAIRHDLVPYEGNAYMREVAKWAEIPVINLQCDVDHPTQTLADLMTLREHRGQD
                     LRGLRVAVSWAYAPSYAKPLSVPQGLLTLLPRFGMDVALAHPPGFDLMPEVVNRAEQG
                     AVQGGGSITHTDDMDVAFEGADVVYPKSWGRLDLFTDEQAALADAARHRDWICDARRM
                     SLAKDDALYMHCLPADRGTEVTDEVIDGPHSVVYDQAENRMHTGKALMALTMA"
     gene            complement(2230..>3978)
                     /locus_tag="VE646_10075"
     CDS             complement(2230..>3978)
                     /locus_tag="VE646_10075"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HZD80371.1"
                     /translation="NYADHALARRDDRVAVVFGNESGDSRSLTAAELARAVGAASAGL
                     RRLGVGRGDRVAAVVSNVPEALIAFLAAASLGAVWSSCSPDFGVRAVTDRFRQIEPTV
                     LVAVDGYRYGGRDFDRLPVVARIRAELPSLRATVMVPVLRRELTEPELDGALTWDELL
                     SEPAMPDPVPVPFDHPLWILYSSGTTGLPKAIVQGHGGILLEHLKSLGLHCDLGPEDR
                     FFWFTTTGWMMWNFLISGLLLGTRVLLYDGSAGHPDLGALWRFADRTGMTYFGTSAPY
                     LQACLKAGLRPRELADLSALRSIGSTGAPLSPEGFRWVYEAVDGDVLLGSISGGTDVC
                     TAFVGSCPLWPVRAGEIQCRCLGAKVEALGPDGTPMIGRVGELALTEPLPSMPTGLWH
                     DPGGERYRRSYFAAFPGAWRHGDWLTVTEHGGCIIHGRSDATLNRGGVRIGTAEFYRV
                     VDAFPEVRESLVIDTGALGRDGELFLFVATAGDVEVDDDLRARIVHELREQLSPRHVP
                     DEILRIAEVPKTLNGKRMEVPVKRILSGARPEEVASPGAMSNPEAIRPFVELARRLAT
                     GQPVTGRDEPGPIRPS"
BASE COUNT          589 a         1410 c         1469 g          510 t
ORIGIN      
        1 tcggtcctgg ccaccggaca ggtgatccgc gggagcgcgc cggtccggga gctggtccag
       61 cgctgccggg cggtcccgct cccccccgag cccgagccca gcgccctccg gcccccgatg
      121 agcttccccg gcggcgccgg caacgtcggg cggggggagg ggctgaggcg gggcgtgggg
      181 ttcgcgctcg gctacaaaaa catcgcgtac agcgaggggt tcgacgactt ccaccaggcc
      241 cgggtgacgt tgtcccgggg gccgagcggt ccgctcgccg aggtccacac ggccggcgtg
      301 gagtgcggcc aggggttctc gacggtcgtc acgcaggtgg cgcgcacgga gctcgggatc
      361 gacacgatcg tcctccatgc ggccgacacc gaggtggact ccgcgggatc gacgtccgcg
      421 tcccgccaga ccttcatggg cggcggggcg gtccggctcg cgtcccgggc ggtgcgcgag
      481 gagctgttcg atcgggctcg ggaagggctc ggcgtcgggg gggagctctc ggtggcggag
      541 ggccggatcc tgaccgacgg cgtggccgtg gccgacgtgg tcgacctcct cgaccagccg
      601 gtggcggcga cgcgcacctt ccatcaccgt cccaccgagc ccctggacgc gaagggtcag
      661 ggggacgtcc acgtgctgtt cgcgttcggc gccctgcgcg cggtcgtcga ggtggacgag
      721 gagctggggc tggtccgggt ggtgcagctg gcatcggccc tggacgtggg gcgggccgtg
      781 aacccccaag gcgtcgaggg ccagagcgag ggcggagcgg cgcagggcct cgggctggcc
      841 ctgatggagg agctccagac ccgcgagggg gtcatcgcca acgcctcgtt caccgactac
      901 ctgatcccca ccacgctgga catgccgtcg gtggtcaccg cgatcgtgga ggaccccgag
      961 cccgaggtcc cgtacggcgc caagggcgtc ggcgagatct cgacgatcgt gtcgaccgcc
     1021 gcgatcgtgg cggcgatccg gaacgcgacc ggccgggtgt tgaaccgggc accggtccgg
     1081 cccgaccaga tcgcgggcct ggagggaccg gtgccggcgc ggccctggcc gccggtcccg
     1141 gaggtcccga gccaggagcc ggtgccggcg accttcggcg tgccgctggg tcagagcggc
     1201 ctgtccgagc gtgacgcgac ccggcgatga gggggcgagg ggggggaccg acgatggtgg
     1261 aacgcgcatc gctgagcggc cgggacttca tcgagaccct ggactggacc acgggggaga
     1321 tcgatgaggt cgagtggctg gccacggagc tcaagcgcaa gttccgaagc ggcgagccgc
     1381 accggctgct gccggacaag acgctgttca tgctgttcct ggacaagtcc acccggaccc
     1441 gcaacgcctt cgaggcaggc atgacccagc tcggcggcca cgcgcacttc ctcgatgccg
     1501 agaagacgca ggtggcgcac ggcgagagcc ccaaggacat ggggatcatc ctgtcccggt
     1561 acggtcacgg gctggcgatc cggcacgacc tcgtccccta cgaggggaac gcgtacatgc
     1621 gcgaggtggc gaagtgggcc gagatcccgg tgatcaacct gcagtgcgac gtcgatcacc
     1681 ccacccagac cctggccgat ctgatgacgc tccgggagca ccgcggccag gacctccgag
     1741 ggctgcgggt ggccgtgtcg tgggcctacg ccccctccta cgccaagccc ctatcggtgc
     1801 cgcaggggct gctcaccttg ctgccccggt tcgggatgga cgtggccctg gcgcaccccc
     1861 ccgggttcga cctgatgccc gaggtcgtga accgggccga gcagggcgcg gtccagggcg
     1921 ggggatcgat cacccacacc gacgacatgg acgtcgcgtt cgagggcgcc gacgtggtct
     1981 accccaagtc gtgggggcgc ctggacctgt tcaccgacga gcaggcggcg ctggccgacg
     2041 cggcgaggca ccgggactgg atctgcgacg cccggaggat gtccctggcc aaggacgacg
     2101 cgctgtacat gcattgcctg ccggcggacc ggggcaccga ggtcaccgat gaggtgatcg
     2161 acggaccgca ctccgtggtc tacgaccagg ccgagaaccg gatgcacacc ggcaaggccc
     2221 tgatggccct cacgatggcc tgatcggacc gggctcgtcc cggccggtga ccggctggcc
     2281 cgtcgccagc cggcgggcca gttccacgaa ggggcggatc gcctcggggt tgctcatcgc
     2341 gccggggctc gcgacctcct ccggccgcgc ccccgagagg atccgcttca ccggcacctc
     2401 catccgcttg ccgttcaggg tcttcgggac ctcggcgatc cggaggatct cgtcgggcac
     2461 gtgacgcggg gagagctgct cgcggagctc gtgcacgatt cgggcccgga gatcgtcatc
     2521 cacctcgacg tccccggcgg tggcgacgaa caggaacagc tccccgtccc ggcccagcgc
     2581 gcccgtgtcg atcaccaggc tctcgcggac ctcggggaag gcgtcgacca cccggtagaa
     2641 ctcggccgtt ccgatccgca ccccgcctcg gttgagcgtg gcgtcggatc gaccgtggat
     2701 gatgcagccg ccgtgctcgg tcaccgtgag ccagtcgccg tgacgccacg cgcccgggaa
     2761 cgccgcgaag tagctgcgcc ggtaccgctc cccgccggga tcgtgccaca ggccggtggg
     2821 catggagggc agcggttcgg tcagggccag ctcgccgacc cgaccgatca tcggcgtgcc
     2881 gtcgggaccg agggcctcga ccttcgcccc caggcaccgg cactggatct cccccgcccg
     2941 caccggccac agggggcacg agccgacgaa ggcggtgcag acgtcggtgc cgccgctgat
     3001 cgacccgagc agcacgtcac cgtccacggc ctcgtagacc caccggaagc cctccggtga
     3061 caggggggcg ccggtggagc cgatggagcg cagggcggag aggtcggcca gctcgcgggg
     3121 ccggagcccc gccttcaggc acgcctgcag gtacggggcg ctggtcccga agtaggtcat
     3181 gccggtccgg tccgcgaacc gccacagggc ccccaggtcc ggatggccgg cgctcccgtc
     3241 gtacagcagc acccgcgtcc cgagcagcag cccggagatc aggaagttcc acatcatcca
     3301 gccggtggtg gtgaaccaga agaaccggtc ctcgggcccg aggtcgcagt ggaggccgag
     3361 cgacttcagg tgctccagca ggatcccgcc gtggccctgg acgatggcct tgggcagccc
     3421 ggtggttccc gaggagtaca ggatccacag ggggtggtcg aacggcaccg gcaccgggtc
     3481 gggcatcgcc ggctcgctca gcagctcgtc ccacgtcagg gcgccatcga gctccggctc
     3541 cgtgagctcc cgtcgcagga ccgggaccat caccgtggcc cgcagcgaag gcagctcggc
     3601 ccggatccgt gccaccaccg ggagccggtc gaagtctcga cccccgtagc ggtagccgtc
     3661 caccgccacc aggaccgtcg gctcgatctg ccggaagcga tcggtgacgg cccggactcc
     3721 gaagtcgggt gagcaggagg accacacggc gcccaggctc gcggcggcca ggaaggcgat
     3781 cagggcctcg ggcacgttcg acacgacggc cgccacccgg tcgccacgcc ccacccccag
     3841 ccggcggagc ccggccgacg cggctccaac ggcgcgcgcc agctcggccg ccgtgagcga
     3901 tctcgagtcg cccgattcgt tcccgaacac cacggcgacg cgatcgtcac gacgggccag
     3961 cgcgtggtcg gcgtagtt
//
LOCUS       DASQQQ010000267         2477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_188144, whole genome shotgun sequence.
ACCESSION   DASQQQ010000267 DASQQQ010000000
VERSION     DASQQQ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188144"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..824)
                     /locus_tag="VE646_10080"
     CDS             complement(276..824)
                     /locus_tag="VE646_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HZD80372.1"
                     /translation="MGAPEPEQTAIAGVRLLRLAPHADDRGSLVETYRRAWLPTQARE
                     AVQANLSISRPGVLRGLHWHREQADYWSVVSGTAFVALVDLREGSPTRLATVQRRIDA
                     ARERIGLWIPPGVAHGFHAETEVHLLYLVDAYFSGDDEWGLAWDDPAHGVNWPSTSPV
                     LSERDRANPSLDEVLRDPPPYR"
     gene            870..1262
                     /locus_tag="VE646_10085"
     CDS             870..1262
                     /locus_tag="VE646_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HZD80373.1"
                     /translation="MAERSRDWIDQAHRDLEHARVSMESGHFEWACFAAQQAAEKAAR
                     AVYERLAGVAWGHSVTELLKGLRERTEVPGELLDLARALDRFYVPARYPNGLAAGSPK
                     DFYTEEDAEGAVRDSGALVRFCEGLLAG"
     gene            1240..1566
                     /locus_tag="VE646_10090"
     CDS             1240..1566
                     /locus_tag="VE646_10090"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZD80374.1"
                     /translation="MRVFWLDRAEALQRLRAAASRLVERPDVRAVYLFGSLAQDRAVP
                     GSDADVLVLLERSDRRWVDRPLEFGPWFDGVGMPVELFCYTPEEADRAPLARTARETG
                     IALAVA"
     gene            1747..>2477
                     /locus_tag="VE646_10095"
     CDS             1747..>2477
                     /locus_tag="VE646_10095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZD80375.1"
                     /translation="MAISDAELRIEQRDHVALDDRMLVLDFQAGDPEAFVEIHRRYGG
                     LARHVCRRFLPNPQDADEAFQETMIRVFQGLYRFNGRYALQPWIARIATNVSLDQLRT
                     AARRPAVENGTIEDGEWPDVADGPEVAYERLVQRDLVLSVLEGMPENHRAALVLRELD
                     GRSHQEIAKALDITPAQAKALIHRAKGSFRRGWLRAMSERGGFAGVAILPLLWLLKAG
                     DLARRFVDRAGHATQVAQVATPEVVT"
BASE COUNT          360 a          853 c          917 g          347 t
ORIGIN      
        1 ggagccggcg ggatatcgcg tggcgatggt ggacgtgcgt tcggcgtccg agtatggcga
       61 acggcgcaag agcggagcca ccctcgaccc ccccgaggtt ccggtcgcct tcgcaggcaa
      121 gcttctcgga tcccggctcc acgcgctccc cccagaacag cggtcccggc tcgatatggc
      181 ccgggggcgg cgcgatggac gaccccggat gggtacaagc cctcactgct ctttcggcac
      241 ggaggtcacc tacctgaagc ccccgagggg accggctacc ggtagggcgg ggggtcccgc
      301 aggacctcgt ccagcgaggg gttcgcccgg tcgcgctcgg acaggaccgg ggacgtcgag
      361 ggccagttca cgccgtgggc agggtcgtcc cacgccaggc cccactcatc gtcccccgag
      421 aagtaggcgt cgaccaggta caggaggtgg acctcggtct ccgcgtggaa gccgtgggcc
      481 acgcccggtg ggatccacag gccgatccgt tctctggcgg catcgatccg ccgctggacg
      541 gtggcgaggc gggtcggcga gccctcccgc aggtcgacga gggcgacgaa cgccgtgccg
      601 gagaccacgg accagtagtc ggcctgctcg cggtgccagt gcaggccgcg caggaccccc
      661 gggcgcgaga tcgacaggtt ggcctggacc gcctcgcgcg cctgggtggg gagccaggcc
      721 cggcggtagg tctccacgag gctgccccga tcgtccgcgt ggggggccag gcggaggagg
      781 cggacccccg cgatggcggt ctgctcgggc tcaggggctc ccatcagtgc ccggatacta
      841 gacccctccg tgttagcgtc cgcgatggga tggccgaacg cagtcgggac tggatcgacc
      901 aggctcaccg ggacctggag cacgcccggg tgagcatgga gagcgggcac ttcgagtggg
      961 cgtgtttcgc cgcccagcag gcggccgaga aggccgcgag ggccgtgtac gagcgactgg
     1021 ccggcgtcgc ctgggggcac tcggtgaccg agctcctgaa ggggctgagg gagcgaacgg
     1081 aggtccccgg tgagttgctc gacctcgccc gggccctcga tcgcttctac gtgccggccc
     1141 gatacccgaa cgggctggcg gcggggagcc cgaaggactt ctacacggag gaggatgccg
     1201 agggtgcggt ccgtgattcg ggagcgctcg ttcgcttctg tgagggtctt ctggctggat
     1261 agggccgagg ccctgcaacg cctgcgcgcc gccgcctcac ggctcgtgga gcgaccggat
     1321 gtccgggccg tgtacctgtt cggttccctc gcccaggacc gcgccgtgcc ggggagcgat
     1381 gccgacgtgc tcgtcctgct cgagcgctcg gaccggcgct gggtggaccg gcccctcgag
     1441 ttcggaccgt ggttcgacgg ggtcgggatg ccggtcgagc tcttctgcta cacgccggag
     1501 gaggcggatc gggcaccgct cgcacggacc gctcgggaga cgggcatcgc cctcgcggtc
     1561 gcctgaccgc gccatccacc ggcccggacg acccgggtcg gatggccgag agcaggcgac
     1621 ggggatgggt gctagagttg gctggacccg ttgactgcga gtgcatagac cgaccgagcc
     1681 ataggccgac caagcccatg aacagcaacc cttccccgtc cgccacgttc ttctaaaggg
     1741 attcccttgg ccatcagcga cgccgaactg cggatcgagc agcgcgacca cgtcgcgctc
     1801 gacgatcgca tgctcgtcct ggacttccag gccggggacc cggaggcctt cgtcgagatc
     1861 caccgtcgct atggcggcct cgcccggcac gtgtgccggc ggttcctccc caacccgcag
     1921 gacgccgacg aggccttcca ggagacgatg atcagggtct tccagggcct gtaccggttc
     1981 aacggccggt acgcgctcca gccgtggatc gcgcgcatcg ccaccaacgt ctcgctcgac
     2041 cagctccgca cggccgcccg ccggccggcg gtggagaacg ggacgatcga ggacggcgag
     2101 tggcccgacg tggccgacgg cccggaggtg gcctacgagc ggctcgtgca gcgcgacctg
     2161 gtcctctcgg tcctcgaggg gatgcccgag aaccaccggg cggcactcgt gctgcgcgag
     2221 ctggacggcc gctcccacca ggagatcgcc aaggcgctgg acatcacgcc cgcgcaggcc
     2281 aaggccctga tccaccgggc caaggggagc ttccgccgcg gctggttgcg ggcgatgagc
     2341 gagcgaggcg ggttcgcggg cgtagccatc ctgccgctgc tgtggctgct caaggcgggt
     2401 gacctcgccc gacggttcgt cgaccgcgcc gggcacgcca cccaggtggc gcaggtcgcc
     2461 accccggagg tggtcac
//
LOCUS       DASQQQ010000268        17336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_347765, whole genome shotgun sequence.
ACCESSION   DASQQQ010000268 DASQQQ010000000
VERSION     DASQQQ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 17336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17336
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347765"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /gene="argS"
                     /locus_tag="VE646_10100"
     CDS             complement(<1..958)
                     /gene="argS"
                     /locus_tag="VE646_10100"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZD80376.1"
                     /translation="MIEDRLSELISGALRSALPEVGADAAPPSIELSKPKQKDFGDFS
                     TNIALALAGRARRSPRDVATAIVAHLPPDDLLERAEVAGPGFINLFVRDDWLYEVLRR
                     VVAQGPRYGWAEPHGHSVNVEFVSANPTGPLHIGHARNAVIGDGLARVMEAAGWRVER
                     EYYFNDAGRQMGLFGASVEARYLQLLGRPVELPEEGYRGEYLIDVARDIAAAFGDSLA
                     DLSEDERHGRLLHEGAERVLAMITRTLERFGVRFDVYFSERTLHGSGEIDETVRRLRE
                     VGHAYEHEGAVFFRSTTFGDDKDRVLIRSNGAPTYFAADCAYL"
     gene            1308..1379
                     /locus_tag="VE646_10105"
     tRNA            1308..1379
                     /locus_tag="VE646_10105"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1341..1343,aa:Arg,seq:ccg)
     gene            1384..2004
                     /gene="pdxH"
                     /locus_tag="VE646_10110"
     CDS             1384..2004
                     /gene="pdxH"
                     /locus_tag="VE646_10110"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HZD80377.1"
                     /translation="MSLPPDDEPLLERDLDPDPIAQFRAWLDQAKAAGIRYPEAMALA
                     TSDPDGRPSVRHVLMRGLDERGLVFFTNYGSRKARQIEQNASGAAVFLWRELDRQVSL
                     TGPIERTSAQESEAYFRTRPREARLGAWASRQSEVVGSREVLEQRYARMERRFAGREV
                     PLPQHWGGYRLQPATVEFWKGRRHRLHDRFRYRRSGTGWLIERLYP"
     gene            complement(2029..2442)
                     /locus_tag="VE646_10115"
     CDS             complement(2029..2442)
                     /locus_tag="VE646_10115"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZD80378.1"
                     /translation="MSQITIRYEGNDRLRVGIRGHELISDQPIEDGGEDAGPSPTELF
                     VGSLAACIAFYAERYLRRHSLPSEGLQVVGSFGFAQDRPARVGTIRLRLVLPPGVPVD
                     RHAGLLAVVEHCTVHNTLRQAPEVEIRLEPGVRAA"
     gene            2590..3933
                     /locus_tag="VE646_10120"
     CDS             2590..3933
                     /locus_tag="VE646_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HZD80379.1"
                     /translation="MTSELRDAIARHMPQTRADLERLVRIPSIAFPGYEEAPVRRSAE
                     VTAEILEAAGTSHVRLLEMPDGIGHPAVFGQIPAPDGVPSVLLYAHHDVQPEGPVDEW
                     DSPPFQPAERDGRLYGRGTADDKCGIVMHAAAVAAHGGRPPVGVKILVEGEEEAGTEH
                     LPFLIEEQRELIAADVAVVADSGNWRHGVPTLTTTLRGVVDCLVEVRALEQAMHSGSY
                     GGPIPDALTSLCRLLATLHDERGNVAVAGLASGPWEGMEYPEHDFRAEAGVRPGVAWI
                     GEGSVAERLWTKPAVSVLGIDAPGVREASNQLVPVARAKVSLRLAPTDEPARALDALA
                     AHLESNAPWGVQVRVERGGEGWGFAVDADGPAYAAAREALGEAWGRPTVDMGSGGSIP
                     LVPMLARTLPGIAVLMLGASDERSNLHSLNESVALDELEKAALAEALLFDRLARG"
     gene            3971..4480
                     /locus_tag="VE646_10125"
     CDS             3971..4480
                     /locus_tag="VE646_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HZD80380.1"
                     /translation="MDTTPPPEEARLRPEVPPGGPPAQPREERADEAQQESITQPAKL
                     LRIASMVRELLEETRQASLDEPGRIRLRDIYDRSVGELKDVLSPDLQEELAMLAPPMD
                     RVPTEAEIRVAQAQLVGWLEGLFHGIQAALFAQQAAARAQFEEIRRRGLPSGQAEQAP
                     PPAGPGQYL"
     gene            complement(4485..5351)
                     /locus_tag="VE646_10130"
     CDS             complement(4485..5351)
                     /locus_tag="VE646_10130"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019069844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD80381.1"
                     /translation="MESRLARIVIKVGSGITLVFLYGPLAILGLYAFNKGITRAWPPD
                     LWSLEWFRAAWRDPALPSALAGSLQAAVGATTTAMLLGSLAAFAVHRFRFFGRDTISF
                     ALVLPIALPGIVTAIALSSAINFGHSIFGLTFSIWTIIIGHATFCVVVVFNNVVARLR
                     RTPTSLVEASMDLGGDGWQTFRYVTFPAIRTALVAGALLAFALSFDEIVVTNFTAGPK
                     LTLPIWIFNNIRTPRQQPVVNVVALVLMIVSVIPVYFAQRLAGGAQVFGGAGGGERSA
                     PSVAPAETAGPG"
     gene            complement(5360..6265)
                     /locus_tag="VE646_10135"
     CDS             complement(5360..6265)
                     /locus_tag="VE646_10135"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD80382.1"
                     /translation="MAATAPQRSEEHRSAFRRTSAALFRHPRAKLALMLSPPLGWLLV
                     VYLVSLSLLLVTSFWRTNVLTSQIDRVWGLQNYQELLRGSAYRTIVIRTVGMATAVTV
                     TDLALAFPISYYMARLATPRTRAMLMLAVVMPLWSNYLVRMFSWKILTAGGGPLEALL
                     GVFGIHKSLAFSNWAVWLTFCYLWLPFAILPLYAAFERVPDSLLEASSDLGARGWMTF
                     RRVIVPILLPGLVAASIFTFSLTLGDYITPNMVGKGYFIGNAIYIYVGTANNLPLAAA
                     FAVVPMVIMAAYLTIARRFGAFEAL"
     gene            complement(6326..7528)
                     /locus_tag="VE646_10140"
     CDS             complement(6326..7528)
                     /locus_tag="VE646_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020498321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZD80383.1"
                     /translation="MRKLRGALILVAALGLVGVACGGGSSGEGGGGGGDGEQPQLKAY
                     DANAQGEGQLSLIAWPGYTQPKIVKPFEEETGCQVTLKYGNTSDEMVNLMRQQGGSLY
                     DGVSASGDATNRLIASGDVAAIDVNSFPGYGNVMTGLQAPAHNTVDGVHYGVPYTWGP
                     NILMYNTDIVKPAPTSWDIVFEADSPYAGKITAYDSPIYIADAAMYLKSHQPDLGITD
                     PYELTQDQFNAAVGLLKQQAGMISKYWALYTDEIDGFESQDMAVGTAWPVNLNLVQAD
                     GKVPVDGLFSPEEGVTGWADTWMMSSKAAHETCMLKWMEYSLRPEVQTVTAEYFGATP
                     SVTDACPQLETDLGKDAALYHCGDDDYLSGIFLWKTPLPDCGDDRGQACVDYSQWTSA
                     WTEIRGAG"
     gene            complement(7545..8603)
                     /locus_tag="VE646_10145"
     CDS             complement(7545..8603)
                     /locus_tag="VE646_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD80384.1"
                     /translation="MAVRLEGVTKRFAEVVAVDQVTLDVIEGEFFSLLGPSGSGKTTS
                     LRMIAGFEAPTAGRVYLDGREVTRMAPYERDVNTVFQDYALFPHMDVEENVGYGLKVK
                     RVPKVEQRERVAEALRMVRLEGFGKRRPAQLSGGQRQRVALARALVNRPRVLLLDEPL
                     GALDLKLRQEMQIELKDIQKRVGLTFIYVTHDQEEALTMSDRLAVFSGGRIEQVGSPA
                     EVYENPATGFVAGFVGTSNVLEGDIARSLTGSPSPFTVRPEKIHLGEPDDPVRADECS
                     VAGRVRDVVYIGALTKYFVELDGDGELVVFRQNLTTSSMEALQVRGRAVKLIWSRESN
                     RPVEGAGGGGPDASSPGP"
     gene            complement(8737..9765)
                     /locus_tag="VE646_10150"
     CDS             complement(8737..9765)
                     /locus_tag="VE646_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase 2"
                     /protein_id="HZD80385.1"
                     /translation="MRPYRSVVKPVFFALPPEAAHGLANRLLGLPLPWERIGGVLDDP
                     ALRGDLAGIALPNPIGLAAGFDKTCRHLDALGRLGFGYVVGGTITRLPRRGNRKPRIV
                     RYRGRRSMANSMGLPNPGADAVARTLSEVPRTAPRIVSIADQDLPDVLATHALLEPHA
                     DGIELNAGCPNVSWGRDRDNEAHLAALVSELGRRRSTPLFVKLPPFRSDVEREVVLAL
                     ARIAQERGTDGLTCSNTYPVREPRLAVGGGGLSGRDLLDDTVRIVAEVHRATGGAISV
                     NACGGIFGPQDAIACLEAGATTVQVYTGMLYEGPGVVGTITAGLSSALERRETSLRSL
                     VGSAHRRP"
     gene            9787..10293
                     /locus_tag="VE646_10155"
     CDS             9787..10293
                     /locus_tag="VE646_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HZD80386.1"
                     /translation="MPDDLAQRLLSASELHGDFVLSSGKRSSIYFDKFRFLTDPALLR
                     EAAHAVTGLLPPVVTHLAAPEGAATLLVAAVALEIGLPIAVVRKAPKQYGMRTRVEGH
                     LPAGAQTALIEDVSTTGHQVLDAARVLEGAGCRIRIIVLAIDRGGAEHLRGAGYEVAS
                     VAQVRPGD"
     gene            complement(10312..11079)
                     /locus_tag="VE646_10160"
     CDS             complement(10312..11079)
                     /locus_tag="VE646_10160"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZD80387.1"
                     /translation="MSTNAGYEEREWKGRTGPFTYLLNPGVFAPTHTSRTIADTLEIE
                     PADTVIDVGCGSGVLSFVAARLGAGRVIGCDISEEAVKVARENARRLGLEDRVEFRVG
                     SLLDPVRGERASVIIGDVSGIPDEIAQVSGWFPDGRAGGPTGSELPAAMLESIHENDC
                     LAPGGRLYLPTGTIQAENRILEVARRIFGAANLQALKSREFPLPDVVAKSKAVARMMK
                     EGILDLHARGSRLLWRLQVWRCELPAVGGSGTQLTSG"
     gene            complement(11146..11637)
                     /locus_tag="VE646_10165"
     CDS             complement(11146..11637)
                     /locus_tag="VE646_10165"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HZD80388.1"
                     /translation="MLEDAKGKTGKPTEKRNKPERPRDELGRPLPWGARNRLHLEDFD
                     ALSLERNHELGREHFKAGRFFPAHEAWETAWKQARGTDDAEFFKGLSQLGAGYVHLLR
                     GNAHGAARLLTRAAGRIGGYPPGHLGVDTPAVAATAERDAQAVRSGSLTPGPDAPVDP
                     PPV"
     gene            11887..13029
                     /locus_tag="VE646_10170"
     CDS             11887..13029
                     /locus_tag="VE646_10170"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZD80389.1"
                     /translation="MPPWIPRLILLVILAAFAAYAAVALLGRLRDLLIWLITALFLSF
                     ALEPAVNWLAKRGWRRGLATGAVLFGLALIFLTMIGLMIPLLVDQVQQLVNELPGWIH
                     RAQQFANQRFGLHINTEQILKRVTSAQIDIGNMASNVASVGATILGVVFQMLTIFLFT
                     FYLVADGPRLRRSVCSVLTPRRQREVLDIWEVAIDKTGGYLYSRLLLAALSALFSFIV
                     LMILGVPFALPLALWMGFVSQFIPVIGTYIAAAVPLLVALLSDPWSALIFLIYVVIYQ
                     QIENYLFSPRITARTMQLHPAVAFFAALAGGLLNGVVGAFLALPAAAIIQAIISSYLT
                     RHEVVETELTRESPGEPSETSPDPETASGEPRGPLARWRRARAENR"
     gene            13070..15169
                     /locus_tag="VE646_10175"
     CDS             13070..15169
                     /locus_tag="VE646_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80390.1"
                     /translation="MSAARITLVPHTHWDREWYEPFDRFLERLVEMMDTLVELADEGF
                     PHFHLDGQTAMIDDYLAVRPERAEDIARLVRQGRLSAGPWVTQTDEFLTSGESHVRNL
                     EMGLARAGELGRPLLVGYLPDQFGHVGQMPQILRAAGLHRAVVWRGVPAAVDRTSFWW
                     EAPDGSRVLAEYLAFGYFLGGDLHRLTDPAELSEALRRKVEHVEPFLASDRVLIMVGS
                     DHAGPDPTLPDRVASAQVPGVEVTVASLERHLDGAVPQGLPTWRGELRSSARAHLLPN
                     VYSARVHQKRERGRLEALLERYAEPLAALVPGSTWPADELDRAWTLMLWNGAHDSVCG
                     CSHDGVARDVDARYDEVRTIAQAIVERSLERLARSVEGSGTLRFNPSPFERNGVPGLG
                     WRVDREPVGLAEDPVPLRAEDGWIRADELAFRLRDEEDMGDLYTFCPAPSGEGASDPL
                     ELVAREGRVRATFEDLTVELSVSRRAGEPFIRLSGLIDNRRPDHRLRLHVRLPAVAEG
                     SVAGSPFELVPRARRSEGGEPEPPSPTWPARHLVLAAGVAAYHEGVFEYEAADRELVI
                     TLLRCVGTISRSYLATRSFAAGPEVPTPEAQMLGRTGFALALQADASLEGVLEAWERF
                     ALPLVLAPAAEGGELPSSGTLLDLEGDCELSNVRRWQGALEVRLWNPRRQDPATCRVA
                     GVEHRLGPARIETIRPG"
     gene            15224..16295
                     /locus_tag="VE646_10180"
                     /pseudo
     CDS             15224..16295
                     /locus_tag="VE646_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            16357..17175
                     /locus_tag="VE646_10185"
     CDS             16357..17175
                     /locus_tag="VE646_10185"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HZD80391.1"
                     /translation="MRRDPSPDDRGTKARLPQLASIARRARGRQWRIVPHPGPIGEGP
                     RAGGRSGALRAAIFGANDGLVSNVSLIMGVAGARVDNAVVILAGVAGLLAGAFSMAAG
                     EYVSMRVQREVFERLIHLEAHEIGSDVDAEREELAELYRRKGISQDLAHRLAEEVMRD
                     PETALDTHAREELGLDPQEGLGSPWAAAASSFITFSVGALLPLVPFFFLTGGWAVITS
                     GVLTLVTLFGVGAGMSYLTGRALWWSGVRMMLIGGAAAGITYLVGSLLNVTVGV"
     gene            complement(17153..>17336)
                     /locus_tag="VE646_10190"
     CDS             complement(17153..>17336)
                     /locus_tag="VE646_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80392.1"
                     /translation="SAARTVVFLVVGERKRSPLRWLLGDRDIPAARVRAARVVVVADP
                     SAARGLGWRFRPRPSR"
BASE COUNT         2452 a         6197 c         6138 g         2549 t
ORIGIN      
        1 cgaggtacgc gcagtcggcc gcgaagtagg tgggcgcgcc gttgctgcgg atcagcacgc
       61 ggtccttgtc gtccccgaac gtcgtggacc ggaagaacac ggccccctcg tgctcgtagg
      121 cgtgaccgac ctcgcggagc cggcggaccg tctcgtcgat ctccccggac ccgtgcaggg
      181 tgcgctcgga gaagtagacg tcgaaccgga cgccgaaccg ctccagcgtg cgggtgatca
      241 tggccagcac gcgttcggcg ccctcgtgca gcagacgccc gtgccgctcg tcctcggaca
      301 ggtccgccag cgagtcgccg aacgccgcgg cgatatcgcg ggccacgtcg atcaggtact
      361 cgccccggta gccctcctcg ggcagctcca ccgggcgccc gagcagctgc aggtaccgcg
      421 cctccaccga cgcgccgaac aggcccatct gccggcccgc atcgttgaag tagtactcgc
      481 gctcgacgcg ccaccccgcg gcctccatca cgcgggccag gccgtcgccg atcaccgcgt
      541 tccgcgcgtg gccgatgtgc aggggcccgg tggggttggc ggagacgaac tccacgttca
      601 cggagtggcc gtgcggctcg gcccacccgt acctgggccc ctgggccacc acccggcgca
      661 gcacctcgta cagccagtcg tcgcggacga acaggttgat gaagccgggg ccggccacct
      721 cggcccgctc gagcaggtcg tcgggcggga ggtgagcgac gatggccgtc gccacgtccc
      781 tgggcgagcg tcgggcacgg ccggccaacg ccagggcgat gttcgtggag aagtctccga
      841 agtccttctg cttcggcttg gagagctcga tggatggcgg ggccgcgtcc gcccccacct
      901 ccgggagggc ggagcggagc gctccggaga tgagctcgga gagccggtcc tcgatcatcg
      961 gcgcccgggg cctctccgct tcgagccgac tcggtcagcg cgcgccggac gatgccgacg
     1021 aggtgagtgg ggtccaacgg ctcggcccgg atggtgctgg cggacccgct ccagggcttg
     1081 ctccccgcgg gacgcgacgt cgacctcgac cccatccggc cggaagccga tccggagcag
     1141 ccggacgatg accggatcgc cgtcgacgat caggatcctg cgggcccgct cgccccccga
     1201 aggagccatg tcccccacga gcccgagcat acccacgcgc tcccgtcgcc gagggtctgt
     1261 gcgcatcgat ggagggccgg ggacgcgttg ctagactcgt gccgagcgcc ctcgtagctc
     1321 agtggataga gcgccggcct ccggagccgg gtgcgcaggt tcgagtcctg ccgagggcac
     1381 cgggtgagcc tgccgccgga cgacgagccg ctcctcgagc gtgacctgga tcccgatccg
     1441 atcgcgcagt tccgggcctg gctcgaccag gcgaaggcgg ccggcatccg ctacccggag
     1501 gcgatggcgc tggccacctc ggaccccgac ggccggccct cggtccgcca cgtgttgatg
     1561 cgcgggctcg acgagcgggg cttggtcttc ttcacgaact acggcagccg caaggcccgg
     1621 cagatcgagc agaacgcctc gggtgcggcg gtgttcctgt ggcgcgagct cgaccgacag
     1681 gtgagcctga ccggcccgat cgagcggacc tcggcgcagg agtccgaggc ctacttccgg
     1741 acccggcccc gcgaggccag gctcggcgcc tgggcatcgc gccagagcga ggtcgtgggc
     1801 tcccgcgagg tcctggagca acggtacgcc cggatggagc gtcggttcgc gggccgcgag
     1861 gtgcccctgc cccaacactg gggcgggtac cgcctgcagc cggccaccgt cgagttctgg
     1921 aagggacggc ggcaccgcct gcacgaccgg ttccgataca ggcgcagcgg cacgggctgg
     1981 ctgatcgagc gcctctatcc ctgaagccct gggctcgtcg atgccggatc aggcggcccg
     2041 gacgccgggt tccagccgga tctccacctc gggcgcctgc ctcaacgtgt tgtggaccgt
     2101 gcagtgctcc accacggcca gcagccccgc gtggcggtcg acgggcacgc cggggggcag
     2161 gaccagccgg agcctgatgg tccccacccg ggccgggcga tcctgcgcga agccgaacga
     2221 gccgacgacc tgcaggcctt cgctcggcaa cgagtgcctg cgcaggtacc gctccgcgta
     2281 gaaggcgatg caggccgcca ggctccccac gaacagctcc gtggggctgg gccccgcgtc
     2341 ctccccaccg tcctcgatgg gctggtcgct gatcagctcg tgcccccgga tgcccacccg
     2401 gagccggtcg ttgccctcgt atcggatcgt gatctggctc atcgtcacct ctcccctggt
     2461 cccttcccca ggctcgggga tgcgtcgcac atccgagggg gccgaagggc cctgccggag
     2521 gggcacggat ggtccggtgc gatagggttc gcagccgacg gatgacggcc tgaggagggg
     2581 atcgatcaga tgacgagcga gctccgcgac gcgatcgccc ggcacatgcc ccagacgcgc
     2641 gccgatctgg agcgcttggt gcgcatcccc tccatcgcgt tccccgggta cgaggaagcc
     2701 ccggtccgcc gctcggccga ggtcacggcc gagatcctgg aggcagccgg aacgtcccac
     2761 gtccggctgc tcgagatgcc ggacgggatc ggccaccccg cggtcttcgg ccagatcccc
     2821 gcgcccgatg gggttccctc ggtgctgctg tacgcccacc acgacgtcca gcctgaggga
     2881 ccggtggacg agtgggacag cccgccgttc cagcccgcgg agcgggacgg ccgcctgtac
     2941 gggcggggga ccgcggatga caagtgcggg atcgtgatgc acgccgccgc ggtggccgcc
     3001 cacggaggac ggccgccggt gggcgtgaag atcctggtgg agggcgagga ggaagccggc
     3061 accgagcacc tccccttcct gatcgaggag cagcgagagc tcatcgcggc cgacgtggcg
     3121 gtcgtggccg actcgggcaa ctggcgccac ggggtgccca ccctgaccac gaccttgcgc
     3181 ggtgtcgtgg attgcctggt ggaggtgcgg gccctggagc aggcgatgca cagcggctcc
     3241 tacggcggcc ccatccccga tgccctcacc tccctctgcc ggctcctggc caccctgcac
     3301 gacgagcggg ggaacgtggc cgtggctgga ctggcctcgg gtccctggga ggggatggag
     3361 tatccggaac acgacttccg ggcggaggcg ggcgtccgcc ccggcgtggc gtggatcggc
     3421 gagggctcgg tggccgagcg tctgtggacg aaacccgccg tctcggtgct gggcatcgac
     3481 gcccccgggg tgcgcgaagc ctcgaaccag ctcgtgccgg tggctcgggc caaggtgagc
     3541 ctgcgcctgg ctcccaccga cgaaccggcc cgggcgctcg atgcgctggc ggcccacctg
     3601 gagtcgaacg cgccgtgggg ggtccaggtg cgggtggagc gcggtggcga gggctggggc
     3661 ttcgccgtcg atgcggacgg acccgcgtac gccgcagccc gtgaggcgct cggggaggcc
     3721 tgggggcgac ccacggtcga catgggctcc ggtgggtcga tcccgctggt gccgatgctg
     3781 gcccggaccc tcccgggcat cgcggtgctg atgcttggag cctccgacga gcgctcgaac
     3841 ctgcactcgc tcaacgagag cgtggccctg gacgagctgg agaaggcggc gttggccgag
     3901 gccctgctgt tcgatcgcct ggcccgcgga tgaggccctg cgcgtccccc accgctgcta
     3961 ccgtgtgagc atggacacga ccccgcctcc cgaggaggcc cgtctgcgac cggaggtgcc
     4021 tcccggcggt cctcccgccc agccccgtga ggagcgagcc gacgaggccc agcaggaatc
     4081 catcacccag ccggccaaac tgctccgcat cgccagcatg gtccgcgagc tgctggagga
     4141 gacccgtcag gcctcgctgg acgagcccgg acggatccgc ctgcgcgaca tctacgaccg
     4201 ttcggtgggc gagctgaagg acgtgctgtc cccggacctg caggaggagc tggcgatgct
     4261 cgctccgccg atggaccggg tccccacgga ggccgagatc cgcgtggcac aggcccagct
     4321 ggtggggtgg ctcgaggggc tgttccacgg gatccaggcg gccctgttcg ctcagcaggc
     4381 cgccgcccgg gcccagttcg aagagatccg ccgccgcggg ctcccctcgg gccaggccga
     4441 gcaggccccg ccccccgccg gccccggaca gtatctgtga cgggctagcc cggaccggcg
     4501 gtctcggcgg gggccacgga cggcgccgag cgctcgccgc cccccgcgcc gccgaacacc
     4561 tgcgctcctc cagccaaccg ctgcgcgaag tacacgggga tgaccgacac gatcatcagc
     4621 accagcgcca ccacgttcac caccggctgc tgacgcggcg tccggatgtt gttgaagatc
     4681 cagatcggga gggtcagctt ggggccggcc gtgaagttcg tcaccacgat ctcgtcgaac
     4741 gacaacgcga acgcgagcag agcgccggcc acaagcgccg tccggatcgc cgggaacgtg
     4801 acgtagcgga aggtctgcca tccgtcgccg cccaggtcca tcgacgcctc caccagtgag
     4861 gtgggggtgc gccggaggcg agcgacgacg ttgttgaaca ccaccaccac gcagaacgtc
     4921 gcgtgcccga tgatgatggt ccagatcgag aaggtcaggc cgaagatgct gtgcccgaag
     4981 ttgatcgccg aggacaaggc gatcgccgtc acgatccccg gcagggcgat cggcagcacg
     5041 agggcgaacg agatcgtgtc ccgaccgaag aaccggaacc ggtggacagc gaaggcggcc
     5101 agggagccca ggagcatcgc cgtcgtggtg gcgcccaccg ccgcctgcag ggaaccggcc
     5161 agcgcgctgg ggagggccgg atcccgccag gcggcgcgga accactccag ggaccacaga
     5221 tcgggcggcc acgccctggt gatgcccttg ttgaaggcgt agaggccgag gatcgccagg
     5281 ggcccgtaca ggaacacgag ggtgatcccc gaccccacct tgatcacgat ccgcgccaga
     5341 cgcgattcca ccaaccctcc tacaaggcct cgaacgcacc gaaccggcgt gcgatcgtga
     5401 ggtacgccgc catgatcacc atggggacca ccgcgaaggc cgccgccagc gggaggttgt
     5461 tcgccgtgcc cacgtagatg tagatggcgt tgccgatgaa gtagcccttg cccaccatgt
     5521 tgggggtgat gtagtcaccg agggtcaacg agaaggtgaa gatggaagcg gcgacgagcc
     5581 cggggagcag gatgggcacg atcacccgcc ggaaggtcat ccatccccgc gcgcccaagt
     5641 cgctcgaggc ctccagcagc gagtccggca cccgctcgaa cgccgcgtac aggggcagga
     5701 tcgcgaaggg cagccacaga tagcagaacg tcagccacac ggcccagttg ctgaaggcca
     5761 ggctcttgtg gatgccgaag acaccgagca gcgcttcgag cggccctccg ccggcggtca
     5821 ggatcttcca ggagaacatc cgcaccaggt agttggacca gagcggcatg accaccgcaa
     5881 gcatcagcat ggctcgcgtc cgcggcgtgg ccaggcgcgc catgtagtag gagatgggga
     5941 acgccagcgc gaggtccgtc acggtgaccg cggtagccat ccccaccgtc ctgatgacga
     6001 tcgttcggta cgcactgccg cgcagcagct cctgatagtt ctgcagcccc cacacgcggt
     6061 cgatctgcga ggtgaggacg ttggtcctcc agaacgacgt gaccagcagc agcgagaggg
     6121 acacgaggta gacgaccagc agccacccca ggggcggcga cagcatgaga gccagcttgg
     6181 cccggggatg acggaagagt gccgcagagg tccggcggaa ggcggaccgg tgctcttcgg
     6241 atcgctgggg cgctgtggca gccatggtgg acccgtggaa gggtgacggg gcccacggag
     6301 cgggcccgtc accgatacct ctttcctatc cggcccctcg gatctcggtc caggcgctcg
     6361 tccactgcga gtagtcgacg caggcctggc cgcggtcatc cccgcagtcg ggcagcgggg
     6421 tcttccacag gaagatgccc gagaggtagt cgtcgtcccc acagtggtag agggcggcgt
     6481 ccttccccag gtcggtctcg agctgcgggc acgcgtccgt cacggacggc gtggcgccga
     6541 agtactcggc cgtgaccgtc tgcacctcgg gacggagcga gtactccatc cacttcaaca
     6601 tgcaggtctc gtgggccgcc ttggaggaca tcatccaggt gtccgcccag ccggtgaccc
     6661 cttcctccgg cgagaacagc ccgtccaccg ggaccttgcc gtcggcctgg accaggttga
     6721 gattgacggg ccaggcggtg cccaccgcca tgtcctggga ttcgaacccg tcgatctcgt
     6781 ccgtgtacag ggcccagtac ttgctgatca tgccggcctg ctgcttgagg aggccgaccg
     6841 ccgcgttgaa ctggtcttgc gtcagctcgt acgggtcggt gatccccagg tccggctggt
     6901 gggacttcag gtacatcgct gcatcggcga tgtagatcgg gctgtcgtag gcggtgatct
     6961 tccccgcgta cggcgaatcg gcctcgaaga cgatgtccca gctggtgggc gccggcttca
     7021 cgatgtccgt gttgtacatc aggatgttcg ggccccaggt gtacgggacc ccgtagtgga
     7081 caccgtccac cgtgttgtgg gcgggcgcct ggagaccggt catcacgttc ccgtaacccg
     7141 ggaacgagtt cacgtcgatg gccgcaacgt ctccgctcgc gatcagccgg ttggtggcat
     7201 cccccgaggc cgacacgccg tcgtagagcg agcccccctg ctgtcgcatg aggttgacca
     7261 tctcatccga ggtgttgccg tacttgaggg tgacctggca gccggtctcc tcctcgaagg
     7321 gcttgacgat cttgggctgg gtgtatccgg gccaggcgat gaggctgagc tgcccctcgc
     7381 cctgcgcgtt ggcgtcgtag gccttgagct ggggctgctc gccgtcgcct cctcctccgc
     7441 cgccctctcc ggagcttccg cccccgcatg ccacgcccac cagccccagg gcggccacga
     7501 ggatcaacgc ccccctcagc ttcctcatcc ggttcctcct ccccctacgg gccgggcgac
     7561 gacgcgtccg gccctccccc tccggccccc tcgaccggac gattgctctc acggctccag
     7621 atcagcttca cggcgcgccc ccgtacctgc agcgcctcca tcgacgacgt ggtgaggttc
     7681 tggcggaaga ccaccagctc cccgtcgccg tccagctcca cgaagtactt cgtgagcgcc
     7741 ccgatgtaga cgacgtcgcg cacccgtccg gcgaccgagc actcgtccgc gcggaccggg
     7801 tcgtccggtt cgcccaggtg gatcttctcc ggccgaacgg tgaacggcga gggcgaaccg
     7861 gtgagcgagc gcgcgatatc gccctccagg acgttggacg tgccgacgaa gccggcaacg
     7921 aagcccgtgg ccgggttctc gtagacctcg gcgggactgc cgacctgctc gatcctgccg
     7981 ccgctgaaca ccgcgaggcg gtcgctcatc gtgagcgcct cctcctgatc gtgggtcacg
     8041 tagatgaacg tgagccccac ccgcttctgg atgtccttga gctcgatctg catctcctgg
     8101 cggagcttca gatccagggc cccgagcggc tcgtccagga gcagcacccg gggccggttg
     8161 accaaggcgc gggccagcgc cacccgctgc cgttggcccc ccgagagctg ggccggccgc
     8221 cgcttcccga acccctccag gcgcaccatc cgcagggcct cggccacgcg ctcccgctgc
     8281 tcgaccttgg gcacccgctt caccttcagc ccgtagccga cgttctcctc cacgtccatg
     8341 tgggggaaga gcgcgtagtc ctggaagacg gtgttcacgt cccgctcgta gggcgccatc
     8401 cgcgtgacct cgcggccgtc gaggtagacg cgaccggccg tgggcgcctc gaacccggcg
     8461 atcatccgta gcgacgtggt cttgccggat ccggaggggc ccagcagcga gaagaactcc
     8521 ccttcgatca catccagcgt gacctgatcg accgccacca cctcggcgaa ccgcttggtg
     8581 acgccctcga gccggaccgc catggatccg tggtctggct tccggacgtc gctcacccgg
     8641 ccccgctggg cccgttgcgc caacccctcc tcctcctcgc gaacatgcat cttgaacggg
     8701 cggtccgacg ggggcaagac acccgagtgg cgaacctcac ggccgccggt gtgccgaccc
     8761 gaccagcgag cgcaggctcg tctcacgccg ctcgagggcg gaggatagcc ccgcggtgat
     8821 cgtcccgacg accccggggc cctcgtagag catccccgta tagacctgca cggtcgtggc
     8881 gccggcctcc aggcaggcga tcgcgtcctg gggcccgaag atgccgccgc acgcgttcac
     8941 cgagatcgcg ccgccggtgg cccggtgcac ctcggccacg atccggacgg tgtcgtccag
     9001 gaggtcgcgc cccgagagcc cacccccgcc gaccgccaga cgtggctcgc ggaccggata
     9061 ggtgttcgag cacgtgagcc cgtccgtacc acgctcctgc gcgatccgcg ccagggccag
     9121 gaccacctcg cgctcgacgt ccgagcggaa cggcggcagc ttcacgaaga ggggtgtcga
     9181 ccggcgcctc cccagctcgg agaccagcgc ggccaggtgc gcctcgttgt cgcggtcccg
     9241 tccccaggag acgttcgggc aaccggcgtt cagctcgatg ccgtcggcgt ggggctcgag
     9301 cagggcgtgc gtggccagga catcgggcag gtcctggtcg gcgatgctga cgatcctggg
     9361 ggcggtccgc gggacctcgc tcaaggtgcg ggcgacggcg tcggcgccgg ggttcggcag
     9421 gcccatggag ttggccatgg accgccgccc gcgataccgg acgatcctgg gcttgcggtt
     9481 cccgcgcctt ggcaggcggg tgatcgtccc gcccaccacg tagccgaacc ccagccgccc
     9541 gagcgcgtcg aggtgacgac aggtcttgtc gaagccggcg gccaggccga tcggattggg
     9601 gagggcgatc ccggccaggt cccccctcag cgccggatca tccagaaccc cgccgatccg
     9661 ctcccagggc agcggcaggc ccaggagccg gttggcgagc ccgtgtgccg cctcgggggg
     9721 gagggcgaag aacaccggct tcaccacgct gcggtacggg cgcatcggac gcctatgcta
     9781 cccgccgtgc ccgacgacct ggcccagcgg ctcctcagcg cttcggagct ccacggggac
     9841 ttcgtcctgt cctcggggaa gcgctcgagc atctacttcg acaagttccg cttcctgacc
     9901 gatccggcgt tgctacgcga ggcggcccac gccgtgaccg gcctgctgcc gccggtggtg
     9961 acgcatctgg ccgcacccga gggcgccgca accctgctgg tggcggcggt ggccctggag
    10021 atcggcctac cgatcgccgt ggtgcgcaag gcccccaagc agtacgggat gcgcacgcgg
    10081 gtggagggcc acctgcccgc cggcgcccag accgcgctga tcgaggacgt ctccaccacg
    10141 ggccaccagg tgctggatgc ggcccgggtg ctggaggggg ccgggtgtcg catccggatc
    10201 atcgtgctgg ccatcgaccg gggaggcgcc gagcacctcc gaggcgcggg atacgaggtc
    10261 gcctccgtgg cccaggtgcg tcccggggac tgaggccgtc cgcgcccgga ctcagccaga
    10321 ggtcagctgg gtgcccgagc cgcccaccgc cggtagctcg caccgccaga cctgcagccg
    10381 ccagagcagg cgactgcccc gggcatgcag atcgaggatg ccctccttca tcatccgggc
    10441 cacggccttg gacttggcca ccacgtcggg gagcgggaac tcccgggact tcagggcctg
    10501 caggttggcc gcgccgaaga tccgcctggc cacctccagg atccggttct cggcctggat
    10561 cgtgcccgtg ggcaggtaca ggcgaccgcc gggagccagg cagtcgttct cgtggatgct
    10621 ctcgagcatg gccgccggca gctcggaccc ggtgggcccg ccggcccgtc cgtcggggaa
    10681 ccagccggag acctgcgcga tctcatcggg gatcccggag acgtcgccga tgatcacgct
    10741 ggcccgctcc ccccgcaccg gatccaggag gctccccacc cggaactcga cccgatcctc
    10801 gaggcccagg cgccgggcgt tctcccgggc gaccttcacg gcctcctccg agatgtcgca
    10861 gccgatcacc cggccggcgc ccagcctggc cgccacgaac gacaggacgc cgctgccgca
    10921 tccgacgtcg atcacggtgt cggccggctc gatctcgagg gtgtcggcga tcgttcggct
    10981 ggtgtgcgtg ggggcgaaca cgccggggtt cagcaggtag gtgaaggggc cggtccgccc
    11041 cttccattcc cgttcttcgt atccggcgtt cgtgctcatc gcgcgctcat ggtgcgcagg
    11101 ctgcggggaa acagtcaacc ggacggtacc cggagccggc cccggtcaca ccgggggagg
    11161 atcgaccgga gcgtccggac ccggggtcag cgatccggat cggaccgcct gggcgtcccg
    11221 ctcggcggtg gccgcgaccg ccggggtgtc cacgcccagg tgtccgggcg ggtatccacc
    11281 gatcctcccc gcggctcgcg tcagcaggcg ggccgccccg tgggcgttcc cccggagcag
    11341 gtggacgtag cccgctccca gctgggaaag gcccttgaag aactcggcgt cgtcggtgcc
    11401 ccgggcctgt ttccaggccg tctcccaggc ctcgtgggcc gggaagaacc gtccggcctt
    11461 gaagtgctcc cgccccagtt cgtggttgcg ctccagggac aaggcgtcga agtcctctag
    11521 gtggaggcgg ttccgagcgc cccatggaag gggccggccg agctcgtccc tgggtcgctc
    11581 cggcttgttc cgcttctccg tgggcttgcc ggtcttgccc ttcgcgtcct cgagcatccg
    11641 gtcatcgtag cggccctcac gccgttcacg gtcccacccg ctcggcacga gcccccgcgc
    11701 tcgcgggcgt cggaggccca ggtactggcc atgatcgtgc gtcgaggcta ccctcaccgg
    11761 acgatggctc gtcccgaacg atcgacgccc gaggaccagc cgacggaacc gcccgtcgag
    11821 gctccggccg cgcgtccggg gatccctcgg aacgagccgg cgacgcccca gccggatgcc
    11881 gcacgcatgc ctccatggat cccgcggctg atcctgctgg tcatcctggc ggcgttcgcc
    11941 gcctacgccg ccgtggcgct cctgggccgg ctccgcgacc tgctcatctg gctgatcacc
    12001 gccctgttcc tctcgttcgc actggagccc gcggtgaact ggctggccaa gcgggggtgg
    12061 cgccgtggcc tggccacggg ggcggtgctg ttcggcctcg ccttgatctt cctcacgatg
    12121 atcggcctga tgatcccgct gctggtggac caggtgcagc agttggtcaa tgagcttccc
    12181 ggatggatcc accgggccca gcagttcgcg aaccagcggt tcgggttgca catcaacacc
    12241 gagcagatcc tgaagcgggt caccagcgcc cagatcgaca tcggcaacat ggcctcgaac
    12301 gtggcctccg ttggagccac gatcctcggc gtcgtcttcc agatgctcac gatcttcctg
    12361 ttcacgttct acctggtggc cgacggaccg aggctacgtc ggtcggtgtg ctccgtgctg
    12421 acgccccgcc gtcagcggga ggtgctggac atctgggagg tggccatcga caagaccggc
    12481 ggctacctct actcgcggct gctcctggcg gcgctgtcgg ccttgttctc gttcatcgtg
    12541 ctgatgatcc tgggcgtgcc gttcgcgctg cctcttgcgc tgtggatggg gttcgtctcg
    12601 cagttcatcc ccgtgatcgg cacgtacatc gccgcggccg tgcccctgtt ggtggccctg
    12661 ctctccgatc cctggtctgc gctgatcttc ctgatctacg tggtgatcta ccagcagatc
    12721 gagaactacc tcttcagccc gaggatcacc gcgcgcacga tgcagctgca tccggcggtg
    12781 gccttcttcg ccgcgctggc cgggggcctc ctgaacggcg tggtgggcgc gttcctggca
    12841 ctgccggcgg ccgcgatcat ccaggccatc atctccagct acctcacccg ccacgaggtc
    12901 gtcgagacgg agctgacccg cgagtcgccg ggggagccct ccgagacgtc ccccgatccg
    12961 gagaccgcct ccggtgagcc tcgcggaccg ctggctcggt ggcgtcgcgc ccgagcggag
    13021 aaccgctgag tccacaaccg ggccccgaag cgcgtgagac actcccgccg tgagcgccgc
    13081 ccggatcacc ctggtccccc acacgcattg ggatcgggag tggtacgagc cgttcgatcg
    13141 gttcctggaa cgtctggtgg agatgatgga caccctcgtc gagctggcgg acgaggggtt
    13201 cccccacttc cacctcgacg ggcagacggc catgatcgac gactacctcg ccgtgcggcc
    13261 agagcgggcc gaggacatcg cgcgcctggt gcgccagggc cgactctcgg ccggaccgtg
    13321 ggtgacccag acggacgagt tcctgacctc cggcgagtcg cacgtccgca acctggagat
    13381 gggcctggcc cgggccgggg agctggggcg tcctctcctc gtggggtacc tgcccgatca
    13441 gttcggtcac gtcgggcaga tgccgcagat cctgcgtgcg gccggcctcc accgggccgt
    13501 cgtgtggagg ggcgtgccgg ccgccgtcga tcgcacgtcg ttctggtggg aggcgccgga
    13561 cggaagtcgc gtgctggccg agtacctggc cttcggctac ttcctgggcg gtgacctgca
    13621 ccggctcacc gaccccgcgg agctctcgga ggccctccgc cgcaaggtgg agcacgtgga
    13681 gccgttcctg gcgtcggacc gcgtcctgat catggtgggg tccgaccacg cggggccgga
    13741 cccgaccctg cccgatcggg tggcgtcggc gcaggtgccc ggcgtggagg tgaccgtcgc
    13801 gtccctggaa cggcacctcg atggagcggt gccgcagggt ctgcccacct ggcggggaga
    13861 gctgcgctcc tcggctcggg ctcacctgtt gccgaacgtc tactcggcgc gcgtgcacca
    13921 gaagcgggag cgcggccgcc tggaagcgct gctggaacgc tacgcggagc cgctggcggc
    13981 cctggtgccc gggtcgacct ggccggccga cgagctggac cgggcctgga cgctgatgct
    14041 ctggaacggg gcgcacgact ccgtgtgcgg gtgctcgcac gacggcgtgg cgcgcgacgt
    14101 cgatgcccga tacgacgagg tccgcacgat cgcccaggcc atcgtggagc gctcgctgga
    14161 gcggctggct cgatccgtgg aagggtcggg caccctccgc ttcaacccct cgccgttcga
    14221 gcgcaacggc gtgcccgggc tgggctggcg ggtggatcgt gagcccgtgg ggttggccga
    14281 ggacccggtc cccctccgtg ccgaggacgg ctggatccgc gcggacgaac ttgcgttccg
    14341 cttgcgtgac gaggaggaca tgggcgacct gtacacgttc tgtccggcac cgtccgggga
    14401 gggggcctcg gatccgctcg agctcgtggc ccgggaggga cgggttcggg cgaccttcga
    14461 agacctgacg gtcgagctct cggtgagccg acgggcgggc gagccgttca tccggctctc
    14521 gggcctgatc gacaaccgga ggcccgacca ccgactgcgg ctgcacgtgc ggctgcccgc
    14581 ggtcgcggag ggctcggtgg ccgggtcccc cttcgagctc gtcccccggg cacggcggtc
    14641 cgagggcggc gagcccgagc cgccctcccc gacctggccg gcccgccatc tggtcctggc
    14701 tgccggtgtg gcggcctacc acgaaggggt cttcgagtac gaggccgcgg accgggagct
    14761 cgtcatcacg ctgctccgat gcgtggggac gatcagccgc tcgtatctcg ccacgcgctc
    14821 cttcgcggcc ggcccggagg tcccgacccc cgaggcccag atgctcggcc ggaccggctt
    14881 cgctctggcc ctgcaggcgg acgcctccct ggaaggggtg ctggaggcgt gggaacgctt
    14941 cgcgctgccc ctggttcttg cgccggcggc ggagggcggg gagctcccct cgtccggcac
    15001 cctgctggac ctcgaggggg actgcgagct ctcgaacgtg cgccgatggc agggggccct
    15061 cgaggtccgg ctctggaacc cgcgccggca ggacccggcg acctgccgcg tggccggggt
    15121 ggagcaccgg ctcggaccgg cccggatcga gacgatccga ccggggtagg ctctcgcgac
    15181 cgtgcgccag gacttcgacg tcatcgtggt gggcggcggc gccatgggga cggccgcggc
    15241 gcggagcctg accgaacggg gtcggagcgt gctcctcctc gagcggttcg cgatcggcca
    15301 tgcccgcggc agctccgggg ggccaaccag gatcttccgc ctctcgtacg accatcccga
    15361 ctacgtccgc atggcccggg tggccctgcg cgagtggcgg gagctcgagt cccgggccgg
    15421 cgagcctctg atgatcacca ccggcggcct ggacgtggga cccggatcgc gtcccacggc
    15481 cgaggcgctc cgagcggccg gagagcggct cgagtatctc cccgtggacg ccgtgaaccg
    15541 agcgatggcc cggactccgc cttccccccg gagacgaggt cttcgtgcag gaggacggcg
    15601 gcgtgtgcct ggcggagcgg acggtccggg cgcaggcggg gctggcggtg gagggcgggg
    15661 ccacgctgct cgagcacacc ctggtggtgc gcgtggtccc cacgggcacc cgcgccctgg
    15721 tccacacgga cgccaggacg ttccgagcgc cccaggtggt ggtgacggcg ggatcctggg
    15781 ccgggccgct gctggccgat gcggggatcg ccctccccct gatcccgagc ctcgagcagg
    15841 tgacgtactt ccagctcgac cgtccgtccc cgctgcccac ggtgatcgac tggacgatcc
    15901 accgcgtccg cacgccgtac gtcgtgccgc atcccgagca gccgggcgcc ttcaaggtcg
    15961 cgctccacca ctccggggcg ccggtgaacg cgaacgagcg gtcgttcgag cccgaccccg
    16021 agcgcgtagc ccgggtgacc gagtacacgg cggaccggtt cgccgcccac cacgccacgg
    16081 ccgccacgga gacctgcctg tacacgaaca cgccggacga gggcttcgtg ctggaccggc
    16141 ggggcccgcc ggtcgtgggc tcgccatgca gcggccatgg gttcaagttc acgcccctga
    16201 tcgggcggat cctggccgac ctgatcacgg ggcgatcggc gcccgtgccg ctcgaccggt
    16261 tctcgatcga tcgcctcctg aaccacggca cctgaccggc gcctggggga gcggcgcgtc
    16321 ccggcatcga cgctcgaggc cggtacgctg cccgggatgc gtcgggaccc ctccccggat
    16381 gatcggggaa ctaaggccag gctgccccag ctggcgtcga tcgcccggcg ggcccgcggg
    16441 cggcagtggc ggatcgtgcc gcatccggga cccatcgggg agggaccgcg cgcgggagga
    16501 cgatccggtg ccctacgtgc ggcgatcttc ggcgctaacg atgggctcgt gtcgaacgtc
    16561 tcgctgatca tgggggtcgc gggcgcccgg gtggacaacg ccgtcgtgat cctcgccggc
    16621 gtcgccggcc tgttggccgg ggcgttctcg atggccgccg gcgagtacgt ctcgatgcgg
    16681 gtgcagcgcg aggtgttcga gcgcctgatc cacctcgagg cgcacgagat cggatccgat
    16741 gtggacgccg agcgggagga gctcgccgag ctctaccgcc gcaagggcat ctcccaggat
    16801 ctcgcgcacc gtctggccga ggaggtgatg cgcgatcccg agacggccct ggacacccac
    16861 gcccgggagg agctggggct cgatccgcag gagggcctcg gctcgccgtg ggccgccgcg
    16921 gcatcgtcct tcatcacgtt ctcggtgggt gcgctcttgc cgctggtccc cttcttcttc
    16981 ctgaccggcg gctgggccgt gatcacctcc ggcgtgctga cgctcgtcac gctgttcggg
    17041 gtgggggccg ggatgtcgta cctcaccgga cgcgccctgt ggtggtccgg cgtgcgcatg
    17101 atgctgatcg gcggggcggc ggccgggatc acctacctgg tgggctccct gctcaacgtg
    17161 acggtcgggg tctgaacctc cacccaaggc cccgggcggc gctgggatcg gccacgacca
    17221 ccacgcgagc ggcccggacc cgggcggccg ggatgtcccg gtcgcccagg agccaccgca
    17281 gcggcgatcg cttgcgctcc cccaccacca ggaacaccac cgttcgggcc gccgac
//
LOCUS       DASQQQ010000269         3394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_47134, whole genome shotgun sequence.
ACCESSION   DASQQQ010000269 DASQQQ010000000
VERSION     DASQQQ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47134"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1374
                     /locus_tag="VE646_10195"
     CDS             115..1374
                     /locus_tag="VE646_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018639424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HZD80393.1"
                     /translation="MLRVIADEHVRQELALTLDEICRRGAERMLAIALEAEVDAYLER
                     HQHARDERGHALVVRNGHARPRSVVAGAGALGVRAPRVDDRRTDPATGERHRFQSSIV
                     PPYARRSPKVAEVLPLLYLHGLSTKDFAPALAEFFGTSAGLSAAAITRLTASWSAERE
                     AFMNRDLSGVDYVYVWADGVHFSVRLGDDDRLCVLVMVGVRTDGTKELIAISDGYRES
                     TESWAELLRDTKRRGMRGPVLATGDGALGFWAAVRDVFPETRWQRDWVHKVANVLNAL
                     PASVQASARRALREIWDAEDKSHADQALDRFARDFAKWPKAVAKLTKDREALLAFYDF
                     PAEHWIHLRTSNPIESTFAPVRARTDLTKGPGSREAGLAMCFKLIEAAEGRWRRVNAP
                     ELVALVRAGAKFVHGRLVERTDQEDAA"
     gene            2102..2182
                     /locus_tag="VE646_10200"
                     /pseudo
     CDS             2102..2182
                     /locus_tag="VE646_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
     gene            complement(2309..2989)
                     /locus_tag="VE646_10205"
     CDS             complement(2309..2989)
                     /locus_tag="VE646_10205"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZD80394.1"
                     /translation="MHGYYAERLADRALERCYQLAPPRVQRYLAAELEFVVAQLRPTD
                     LVLDLGCGYGRAIPAFARAAGFVVGIDTSLPSLEMASRQLRTVPNAAVACMDGTRLGL
                     AGGSFDVVACVQNGICAFHVDQRLLLGEALRMLRPGGTALFSTYAERFWAPRLEWFER
                     QAAAGLIGEIDRERTRPCLIVCTDGFTSGTLSPEEFPALIAGLEVKARSVEVDGSSRF
                     FILRKPSP"
     gene            3127..>3394
                     /locus_tag="VE646_10210"
     CDS             3127..>3394
                     /locus_tag="VE646_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80395.1"
                     /translation="MAKRSRPRIVTKDRLDLEAFPEHVRVALAEVAGAAREGVLALSV
                     ACGLQVVAEMMAADVERICGPRGKHDADRAAYRHGSEERLVPLGG"
BASE COUNT          525 a         1151 c         1221 g          497 t
ORIGIN      
        1 gaggtatgaa cggaagttgt ggatgcggga cctgatgatg ggcgtggcgt accctccggt
       61 cgattcctga tcggagctgc cggcgcggag acgccggcga gaggaggtac gccgatgctc
      121 agggtaatcg cggacgagca cgtccgccag gagctcgccc tcacgctgga tgagatctgt
      181 cgacgcggtg ccgagcggat gctggcgatc gcgctcgagg ccgaggtgga cgcctacctg
      241 gagcgtcacc aacacgcccg ggacgagcgc ggccacgccc tggtggtgcg aaacggccat
      301 gcccgaccaa ggagcgtggt cgcgggagcc ggcgcccttg gggtgcgggc ccccagggtc
      361 gacgatcgac ggacggatcc ggccaccggg gagcggcacc ggttccaaag ctcgatcgtt
      421 cccccgtacg cacgccgctc cccgaaggtg gccgaggtgc tgccgctgct gtacctgcat
      481 gggctttcca ccaaggactt cgcaccggcc ctggccgagt tcttcgggac ctccgcgggg
      541 ctctcggcag ccgccatcac ccggctcacg gcgagctggt cggccgagcg ggaggcgttc
      601 atgaacaggg acctctcggg cgtcgactac gtctacgtgt gggccgacgg agtgcacttc
      661 tcggttcgcc tcggggacga cgatcgcctg tgcgtgctgg tcatggtggg ggttcgcacc
      721 gacggcacca aggagctcat cgccatctcc gacgggtacc gggagtcgac cgagtcctgg
      781 gccgagctgc tccgggacac caaacgccgc gggatgcgcg gcccggtgct cgcaaccggc
      841 gatggagcgc tcgggttctg ggccgcggtc cgcgacgtgt tccccgagac gaggtggcaa
      901 cgggactggg tgcacaaggt ggccaacgtc ctgaacgcgc tgcccgcctc ggtgcaggcc
      961 tccgcccgcc gggctctgcg ggagatctgg gacgccgagg acaagtccca cgccgaccag
     1021 gccctggacc gcttcgcccg cgacttcgcc aagtggccaa aggccgtggc caagctcacc
     1081 aaggaccggg aggctctgct cgccttctac gacttcccgg ccgagcactg gatccacctt
     1141 cggacctcga acccgatcga gtcgacgttc gcaccggtca gggcccggac cgacctcacg
     1201 aaggggccgg ggtccaggga ggcggggctt gcgatgtgct tcaagctgat cgaggccgcg
     1261 gaagggaggt ggaggagggt caacgcgccc gagctcgtgg cgctcgtgcg cgcgggcgcg
     1321 aagttcgtgc acggacggtt ggtcgagcgg accgatcagg aggacgccgc gtgaccatta
     1381 cggattccgt tccacaacta ttgactattc ctcggctgcc ggccgggagg cccgtgaggc
     1441 cctgctggac ccggggatct ccgtcgtcga gcccgcgctg ctggcgagcg atctgggggc
     1501 ttcctcgctc cacgacccca ccgagggcgg tctcgccggc ggtctgcacg agatggcacg
     1561 tgcctcgggg gtcctcctcc ggatcgaccg gcaagccgtc ctctggttcg aggctgggac
     1621 cgctgtgtgc cggaccctcg aggccgaccc ttgggccacc ctcgcatccg ggactttgct
     1681 cgcagccttc cctccggctc tggccgaaac ggctctgcgg agcctcagag cccgggggca
     1741 cgcggcggcg gtcatcggga gggccgagcc tggctcggga gtgcgggacg ccgacgatcg
     1801 cccgatcctc tggccggagc gcgacgaggt ggcccgggtc ctgtccacct tggtctgagg
     1861 gggcggggtc acggctccgt atagtcggca cgcgaggttc gctgggcgga ccccgaagcg
     1921 cccgatctgg gcctagacga ccgcgatggt ggctccagcc gctccgcgcc gacgccatga
     1981 ccgcgtgccc acacacgcac tctgttgccc ttttgggggc tgctttggcc ggctggagtg
     2041 gactgggaga tccccaccac cgtagctccg gaacggctga tcgccatggc atacgggcca
     2101 gttggagaag gtgtggaagg acgccgacat ggacttcatc ctcaccgccc aggaagccat
     2161 caagcacggc gtggtggacg atgtgatcac cagccggaag gtcccgtccg tgcttgcggc
     2221 gaggggccga ggacccgtta gacgggtcct cctcgccaag gccggcgacg cggaggacgg
     2281 gccccccccg ctgccgcggg acctcgacct acggagaggg cttcctcagg atgaagaagc
     2341 ggctggagcc atccacttcc acggagcggg ctttgacctc caaccccgcg atgagggcgg
     2401 ggaactcctc gggcgaaagg gtcccggagg tgaagccgtc ggtgcacacg atcaagcatg
     2461 gacgggtccg ttcccggtcg atctccccga tcaggccggc ggcagcctgg cgctcgaacc
     2521 actccagccg gggtgcccag aacctctcgg cgtaggtgga gaacagagcc gtcccgccgg
     2581 gtcgaagcat ccgcagagcc tcaccgagca gcaggcgctg atcgacgtgg aacgcgcaaa
     2641 tcccgttctg gacgcacgcc accacgtcga acgagccccc ggcaagcccc agccgcgtcc
     2701 catccatgca cgccacggcg gcgttgggga ccgtccggag ctgccgacta gccatctcta
     2761 ggctgggcag cgaggtatcg atccccacca cgaaccccgc ggccctggcg aaggcgggga
     2821 tggcgcggcc gtagccgcat cccaggtcga gcaccaggtc ggtgggacga agctgggcga
     2881 ccacgaactc gagctcggct gccaggtacc gctgcaccct gggcggggcc agctggtagc
     2941 agcgctcgag tgcccggtcg gcgagccgct cggcgtagta gccgtgcatc tgcgaccagg
     3001 ctaactctcc tgtagctctc gattccgagg ttgtcccgta gcccgtagaa gttgagggcg
     3061 gtcctccgcc ccgtccccgc cacctggtgg tagagacggg gtggtctgaa agagaggagg
     3121 accgccatgg caaagcgtag tcgtccgcgg atcgtcacca aggaccgcct ggacctggag
     3181 gccttccccg agcacgtgcg ggtggcactc gccgaggtcg ccggcgccgc tcgcgagggc
     3241 gtgctcgcgc tctcggtcgc ctgcgggctg caggtcgtgg ccgagatgat ggccgccgac
     3301 gtcgagcgga tctgcggccc caggggcaag cacgacgccg accgggccgc ctaccggcac
     3361 ggatccgagg agcgcctggt gccgctcggg ggcg
//
LOCUS       DASQQQ010000270         5422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_217499, whole genome shotgun sequence.
ACCESSION   DASQQQ010000270 DASQQQ010000000
VERSION     DASQQQ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217499"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..2567
                     /locus_tag="VE646_10215"
     CDS             105..2567
                     /locus_tag="VE646_10215"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZD80396.1"
                     /translation="MTDVPDRVELVIVGAGVAGLCVAIRLAELGLGDVLVLDGGPIPI
                     EGPASTNDPTSASGTPIFQTVGVRTTRRLTRRSVAAYGALELDGEPCLSQGGSIEVAI
                     DDASLHALRRRRVLAQADRIEGAELLSPAETADRISLLRAGAVVGSLLIPGDGLLHVG
                     RARAALARRARARGPLLRGGVTVTQLEVADGRVQGVRTDRGTIRCERVVLCAGADAAR
                     LGRIVGTAVPVAAFRYPIVRTQPMPELAADPGHEARYPLFRHPGGALDAHRLGDRCVI
                     RNFRGEPDPLDPATLDPTGAYPSSAGGATERADGLEDVERAAADLLPAVAGRLDPFGT
                     EAGGLSITPDLGPIAGEWVGLRGAWTCRPLGVADAAGLGSVVAEWIAEGEPPIDPSQI
                     DVNRFYPFQTTTAYVRELGIRGYRETTARTQPAGPPSPPRALRRSPFHRRQQALGARF
                     AIEAGWARPRWYEANARLRPSGAPTSPDRHGSDPDRSPIEGWEHLAARSRAALFDLTP
                     LAKFDVMGPDSARFLERLCEARFDGPVGVIVNALMLTPSGGIRADLIVAHKDGELFRL
                     VTEAGAGPQVLAWMRSHLGSEERVTIVERTSSLFALGLWGPRARQVLRSVTEADLSDR
                     AFPPQTARYLNLGDVFPVWAQRISSVGEPGWELYGQLPMGERAWDVLWEAGRAQGLVA
                     AGLGALGSLRLEAGRRTWGLDLDTEHGPAEARLGSLVRSGVGRGASVGGSHAPSLFAC
                     LLPDDPAGTVLGGEPVWADGRLVGRVSSAGFGHSASRTIARAYLPVELAAPGTRVEIG
                     SPDGRLPATVAREQVPAGEGAI"
     gene            complement(2564..3553)
                     /gene="trpS"
                     /locus_tag="VE646_10220"
     CDS             complement(2564..3553)
                     /gene="trpS"
                     /locus_tag="VE646_10220"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZD80397.1"
                     /translation="MRIFSGIQPTGRKHLGNYLGAIRNYVALQDVGDAIYCIVDLHAI
                     TVGYDAPRLPAVLLDTAAILLAAGLDPARCILFRQSDVQEHPELCWMLASVTAYGELT
                     RMHQFKEKSEQQRAFVSAGLLFYPVLQAADILAYRADEVPVGEDQKQHLELSRNVAER
                     FNARYGPTFVVPTHRIPDVAARVMDLQAPQNKMSTSAGTELGTVHVLDEPDVIGRKFR
                     SAVTDTGREVVRGEDKPGISNLIEIMAAARGITPDQVEKEYADAPGYAGFKRDVGHAV
                     IELLAPVRERFTPIREDEVSLQEVLREGAARARAIASETLAVVRARMGLGGPA"
     gene            complement(3615..3965)
                     /locus_tag="VE646_10225"
     CDS             complement(3615..3965)
                     /locus_tag="VE646_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HZD80398.1"
                     /translation="MRASTVRKLCLSLPEAEERETWETATFRVRGKIFVMFSEHEREA
                     WIKATSDEQAALISMDPGVFFFPPYVGKGGWIGVRIAKADRAEVAELIVEAWRLRAPK
                     RLQAAYDDEHPVGA"
     gene            4104..5267
                     /locus_tag="VE646_10230"
     CDS             4104..5267
                     /locus_tag="VE646_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZD80399.1"
                     /translation="MTAEDREIQRKARAFVDEELIPHEVEAELNGGELDPQLTRRHEE
                     RLHELGLSAINMPEELGGDGLTMFQQVLVSEQIGRATNALGWVLHTGPEWAIGVFTPD
                     QAERYLKPIVRCEMVECYAITEPGAGSDVDAIEATARRDGDTYVLNGLKWHVTSFNKA
                     QVAVFQGRLTEGPHAGEHALFLVDLPSPGVRLVRTPAYSHTFAHHHAVVAFEDARVPA
                     ANLVGEEGGGMDFTYAWFRYERLMIAARCCGAAERLIDEATAFAKEREQFGQPIIGFQ
                     AIEHMLADSVTELWAARLMTYRLAQEWDGGEDLKLQHAHCSMAKLYASEMANRVADRA
                     VQVFGGRGYMRENVAERFYRELRVDRIWEGTSEIQRNIIGSQLAKRGAEVLAG"
BASE COUNT          746 a         1831 c         2060 g          785 t
ORIGIN      
        1 tggtggtgct gggcctggtg ttgctcggtc tgggcgggta gcggcggtcc gagaccatcc
       61 ggggacggca tggaccgatg cccgaggcta ggctcaccgc cgacatgacg gacgtgccgg
      121 atcgggtgga gctggtgatc gtgggggccg gggtggccgg gctctgcgtg gcgatccggc
      181 tggccgagct cggcctcggc gatgtcctgg tcctggacgg agggccgatc ccgatcgaag
      241 ggcccgcctc gacgaacgac cccacatcgg cctcgggaac cccgatcttc cagaccgtgg
      301 gcgtgcgcac gacgcgccgg ctgacgcggc gctcggtggc ggcgtacggg gccttggagc
      361 tcgacggaga accgtgcctc tcccagggcg gcagcatcga ggtggcgatc gacgacgcgt
      421 ccctgcacgc gctccgccgg cgccgggtgc tcgcccaggc ggaccggatc gagggggccg
      481 agctgctgtc gccggccgag accgccgatc ggatctccct gctccgtgcc ggtgcggtcg
      541 tcggctcgct gctgatcccc ggggacgggc tcctgcatgt ggggcgggct cgagccgccc
      601 tcgcgcgtcg agcgcgggcc aggggtcccc tcctccgcgg cggggtcacg gtgacccagc
      661 tcgaggtcgc cgacggccgg gtgcagggtg tgcgcaccga ccggggtacg atccggtgcg
      721 agcgcgtcgt gctgtgtgcc ggggccgacg cggcccggct ggggcggata gtggggacgg
      781 ccgtgcccgt cgcggcgttc cggtacccga tcgtgcggac ccagccgatg cccgagctcg
      841 cggccgaccc gggccacgag gctcggtacc ccttgttccg ccatcccggt ggggcgctgg
      901 acgcccatcg gctcggcgac cgctgcgtga tccgcaactt ccgtggcgag cccgatccgc
      961 tcgacccggc gacgctggat cccaccgggg cctacccctc gtcggccggc ggggccaccg
     1021 agcgagccga cgggctggag gacgtggagc gggcggcggc ggacctgctg ccagcggtgg
     1081 cgggtcgcct ggatcccttc ggcacggagg ccggggggct gtcgatcacg ccggacctcg
     1141 ggccgatcgc cggcgagtgg gtggggctcc gcggagcgtg gacgtgtcgg ccgctcgggg
     1201 tggcggacgc ggcggggctc gggagcgtgg tcgcggagtg gatcgccgag ggcgagccgc
     1261 ccatcgaccc atcccagatc gacgtgaacc gcttctaccc gttccagacc acgaccgcct
     1321 acgtccgcga gctgggtatc cgtgggtacc gagagacgac cgccaggacc cagcccgccg
     1381 gaccgccctc accgccccga gcgctccgac gctccccctt tcatcggcgg cagcaggcgc
     1441 tgggggcccg gttcgcgatc gaggccggat gggcccgacc gcgctggtac gaggcgaacg
     1501 cccggctgcg gccgtccggt gctccgacct ccccggaccg gcacggatcc gatcccgacc
     1561 ggtcgccgat cgagggctgg gagcacctcg ccgccagatc gcgggcggcc ctcttcgacc
     1621 tcaccccgct cgcgaagttc gacgtgatgg gtcccgactc cgcgcggttc ctggaacgcc
     1681 tgtgcgaggc gcggttcgac ggccctgtgg gggtgatcgt gaacgccctc atgctgacgc
     1741 cgtccggagg gatccgcgcc gacctgatcg tggcccacaa ggatggcgag ctgttccgac
     1801 tggtcaccga ggcgggcgcc ggcccgcagg tgctggcgtg gatgcgctcg cacctcggct
     1861 ccgaggagcg cgtcacgatc gtggagcgga ccagctcgtt gttcgccctg ggcctgtggg
     1921 gcccgagggc gcgccaggtc cttcgatccg tgaccgaggc cgacctctcg gaccgggcgt
     1981 tcccgcccca gaccgcccgc tacctgaacc tcggcgacgt gttcccggtc tgggcccaga
     2041 ggatctcctc ggtgggggag ccggggtggg agctgtacgg gcagctcccg atgggggagc
     2101 gggcctggga cgtgctgtgg gaggcgggcc gtgcgcaggg gctggtggcg gccgggctcg
     2161 gggccctggg atcgctccgg ctggaggcgg gccggcggac ctgggggctg gacctggaca
     2221 cggagcacgg acccgcggaa gcgaggctcg gatccctggt ccgttcaggg gtgggacgcg
     2281 gggcgtccgt cggcggttcg cacgccccgt ccctcttcgc ctgcttgctg ccggacgatc
     2341 cggcaggcac ggtcttgggt ggcgagccgg tgtgggcgga cggccggctg gtcggtcgcg
     2401 tgtcgtcggc cgggttcggg cactcggcga gccggacgat cgcacgagcg tacctcccgg
     2461 tcgagctcgc ggcgcccggg acccgagtgg agatcgggtc cccggacggc cggctgccgg
     2521 cgacggtggc tcgcgagcag gtgccggccg gggagggagc gatctaggcg ggcccaccca
     2581 ggcccatgcg ggcccggacc acggccaggg tctccgaggc gatggcccga gcccgagcgg
     2641 caccctcgcg caggacctcc tggaggctga cctcgtcctc ccggatgggg gtgaagcgct
     2701 cgcggactgg agccagcagc tcgatcaccg cgtggcccac gtcccgcttg aacccggcgt
     2761 acccgggggc gtccgcgtac tccttctcca cctgatcggg cgttatcccg cgggccgcgg
     2821 ccatgatctc gatcaggttc gagatgccgg gcttgtcctc gccccgaacg acctcccggc
     2881 cggtgtccgt gaccgccgag cggaacttgc ggccgatcac gtcgggctcg tccaggacgt
     2941 ggacggtccc gagctcggtc ccggcgctgg tggacatctt gttctggggc gcctgcaggt
     3001 ccatcacccg agccgccaca tcggggatcc ggtgggtcgg caccacgaag gtcggcccgt
     3061 agcgagcgtt gaagcgttcg gcgacgttgc gggagagctc caggtgctgc ttctggtctt
     3121 cgcccaccgg cacctcatcg gcccggtagg caaggatgtc ggcggcctgc agcaccgggt
     3181 agaagagcag cccggcagag acgaatgccc gctgttgctc cgacttctcc ttgaactggt
     3241 gcatgcgggt gagctcgccg tacgcggtga ccgaggccag catccaacac agctccggat
     3301 gctcctggac gtcggactgg cggaacagga tgcatcgcgc cgggtccagg ccggccgcca
     3361 acaggatggc cgccgtatcg agcaacacgg ccggcaggcg gggggcgtcg tatcccaccg
     3421 tgatggcgtg caggtcgacg atgcagtaga tggcgtcgcc cacgtcctgc agcgccacgt
     3481 agttccggat ggcccccagg tagttcccga ggtgcttgcg accggtgggc tggatgccgg
     3541 agaagatgcg catggtgagg tgcagggtag cggatgcatg ggcccttgga accgcacttc
     3601 cgggacggga gggactatgc cccgaccggg tgctcgtcgt cgtaggcggc ctgcagtcgc
     3661 ttgggagccc tgaggcgcca ggcctccacg atcagctcgg ccacctcggc ccgatcggcc
     3721 ttcgcgatcc gtacaccgat ccacccgccc ttgcccacgt agggggggaa gaagaagacg
     3781 ccaggatcca tggagatcaa ggcggcctgc tcgtcggagg tcgccttgat ccaggcctcg
     3841 cgctcgtgct ccgagaacat cacgaagatc ttgccccgga cccggaaggt cgccgtctcc
     3901 caggtctcgc gctcctcggc ctccggcaag gacaggcaga gcttgcggac ggttgacgcg
     3961 cgcatcagca tcctgccttc ggcaccggcc agagcctctc gccggcggcc gggctcgtca
     4021 acgccggccc agggacggtg cgaccttgaa ccgctgttca ggtggcacgc tatgctgcgc
     4081 ccggaggtga gcccatgtac ccgatgacgg ccgaggaccg ggagatccag cggaaggcgc
     4141 gcgcgttcgt ggacgaggag ctgatccccc acgaggtgga ggccgagctg aacggtgggg
     4201 agctcgaccc gcagctcacc cgccggcacg aggagcgcct acacgagctc ggcctgtcgg
     4261 ccatcaacat gcccgaggag ctgggcgggg acgggctcac gatgttccag caggtgctgg
     4321 tctcggagca gatcggccgg gctacgaacg ccctcgggtg ggtgctgcac acgggtccgg
     4381 agtgggcgat cggcgtgttc actccggatc aggcggagcg gtacctgaag cccatcgtgc
     4441 ggtgcgagat ggtcgagtgc tacgcgatca ccgagccggg ggcgggctcg gacgtcgacg
     4501 cgatcgaggc cacggcccgc cgcgacggag acacctacgt cctgaacggg ctgaagtggc
     4561 acgtcacctc cttcaacaag gcgcaggtcg cggtcttcca ggggcggctc accgagggtc
     4621 cgcacgccgg ggagcacgcg ctgttcctcg tggacctgcc ctcgccgggc gtgcggctgg
     4681 tgcgcacgcc ggcctactcg cacacgtttg cccaccacca cgcggtggtg gccttcgaag
     4741 atgcccgggt cccggccgcg aacctggtgg gcgaggaggg cggcgggatg gacttcacct
     4801 acgcctggtt ccggtacgaa cgactgatga tcgccgcacg gtgctgtggg gcggcagaac
     4861 ggctgatcga cgaggccacg gccttcgcca aggagcgcga gcagttcggc caaccgatca
     4921 tcgggttcca ggcgatcgag cacatgctgg cggactcggt gaccgagctc tgggcggccc
     4981 ggctcatgac ctaccggctg gcccaggagt gggacggggg cgaggacctg aagctccagc
     5041 acgcgcactg ctcgatggcc aagctctacg cgagcgagat ggccaaccgc gtcgcggacc
     5101 gggcggtcca ggtcttcggc ggccgcgggt acatgcgcga gaacgttgcc gagcgcttct
     5161 accgagagct ccgcgtggac cggatctggg agggaacctc cgagatccag cgcaacatca
     5221 tcgggagcca gttggcgaag cgcggtgcgg aggtgctggc gggatgagcg accggatgcg
     5281 gcccgatctg gagcgcttca tgctagcttg atcgatcctg gccccttgcc gtgaagaccc
     5341 cctgcgcgcc aggtgtcggg aggggggcgc atggagcggg aggggagcgc atggagaagg
     5401 atgaccccgc gtacaagggc ca
//
LOCUS       DASQQQ010000271         3297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_160875, whole genome shotgun sequence.
ACCESSION   DASQQQ010000271 DASQQQ010000000
VERSION     DASQQQ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160875"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VE646_10235"
     CDS             <1..294
                     /locus_tag="VE646_10235"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZD80400.1"
                     /translation="EEALGHLAGRDVVTLDLTDLDFIDASGIGAIVRGALGCRIVLWS
                     PRPQIQRVFQLCGIDHHPRLEIRSVSPSPGDGPGAWSRSAALRIHAMVVPVPG"
     gene            complement(299..1687)
                     /locus_tag="VE646_10240"
     CDS             complement(299..1687)
                     /locus_tag="VE646_10240"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HZD80401.1"
                     /translation="MPTGWVNRLPRIDLLDAEQVEAVHRHAIALLEDVGVRISHEPAL
                     AVLRAAGLRAEDDLVRFDRGFVLERLASAPATFGVRGRDRRRDVTFGGRHVVLTCTGG
                     APFVLDGEGRRRPATMKDYLTFTRVVHASEQLHLSQSGVVEPEDVPLPIRHLEMDLAV
                     IRSSDKPYVAGGVSAPTTRDVIDLAAIAFGGRAAMAAEPPMIGIVNPISPLQYDERMA
                     GSLFEYARAGIPVVLMPFVFSGGTTPLSVPAALAQTTAEVLAGVALIQAIRPGTPCVF
                     GASVSELDLHDGQPVYATGGHGLAMLASGQLARHYGLPFRGGGAFTASALPDADAMRE
                     SLTALWPALLSGANFLLHAAGWLEGSLAASLDKLALDLTALRELERIVEARIDMDEEA
                     FAMDAFREAGPGGTFLGTAHTLRHLRRAVADRPTEPVSWQRLREGYEEPSLDPAVEQE
                     LRSFVEARRRAE"
     gene            complement(1810..2505)
                     /locus_tag="VE646_10245"
     CDS             complement(1810..2505)
                     /locus_tag="VE646_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HZD80402.1"
                     /translation="MSEERAVRGADHMTTVAFDVIGTLFDLERPRAALSQVGLPTGSL
                     ELWFAQSLRDAFALSHAGSYRPLADVLEAGLRRLVASTGVRASEEGLGSVMSAFSELD
                     PRPDAPGACRRLANEGVRLLALTNGSERSTRAMLRNAGILDLFSDLLSCDRIRTSKPD
                     PRVYQMAKARARGELWLVAAHAWDVAGATFAGLRTVWVSSVEATYLSAYPLPDVEADD
                     LGQAAGAILDRHR"
     gene            2605..>3297
                     /locus_tag="VE646_10250"
     CDS             2605..>3297
                     /locus_tag="VE646_10250"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HZD80403.1"
                     /translation="MTHPNRTVFHDRHGQLGARFMDFGGWEMPLQYPTGVVQEHLATR
                     NHAGLFDVSHMGRFWISGPGRVAFLQHVLTNNAEALDGRPTGAQYTVIPTEAGGAMDD
                     AYLYRFDEERHLLVVNASNRTKDLEHLRSHLGSFPHVELRDVTEELVMLAVQGPAARG
                     LLSDLVEEGRLPEPIRNAVAEVRIAGAGVLAGRTGYTGEPVGLELFLDRDDGPRLWDL
                     LVERGATPCGLGA"
BASE COUNT          494 a         1219 c         1139 g          445 t
ORIGIN      
        1 gaggaggcat tgggtcacct cgccggccgc gatgtcgtga ccttggacct gaccgacctg
       61 gacttcatcg acgccagcgg catcggcgcc atcgtccggg gagcccttgg atgccggatc
      121 gtgttgtggt ccccgcgacc ccagatccag cgggtgttcc agctgtgcgg catcgaccac
      181 caccctcgcc tggagatccg ctccgtctca ccctcccccg gagacggccc gggggcctgg
      241 agccggtcgg ctgcgctccg catccacgcg atggtcgtgc cggtgcccgg ctagcccatc
      301 actccgcgcg ccggcgggcc tcgacgaacg accgcagctc ctgctccacc gccgggtcga
      361 gcgacggctc ttcgtatccc tcgcgcaacc gctgccacga caccggctcc gtgggtcgat
      421 ccgctaccgc gcgtcgcagg tgacgaaggg tgtgggcggt gccgaggaag gtcccccccg
      481 gacccgcctc ccggaaggcg tccatcgcga acgcttcctc gtccatgtcg atccgcgctt
      541 ccacgatccg ctccagctcc cggagcgcgg tcagatccag ggccagcttg tccagggagg
      601 ccgccaacga gccctccagc cagccggcgg cgtggagcaa aaagttcgcc cccgacagca
      661 gcgccggcca gagcgccgtg agcgactcgc gcatcgcgtc cgcgtcgggc aacgccgaag
      721 cggtgaacgc tcccccacca cggaacggca ggccgtagtg ccgggccagc tgtccggagg
      781 ccagcatggc cagcccgtgc ccgccggtcg cgtagaccgg ctgcccgtcg tgcaggtcga
      841 gctcggacac cgacgctccg aacacgcacg gggtcccggg acggatggcc tggatcagcg
      901 ccacgccggc caggacctcg gccgtggtct gggccagggc cgcgggcacc gagagcggag
      961 tggtcccgcc cgagaacacg aaaggcatca gcacgaccgg gatcccggca cgcgcgtact
     1021 cgaacagcga gccggccatc cgctcgtcgt actggagcgg gctgatcggg ttcacgatcc
     1081 cgatcatcgg tggctcggcc gccatcgccg cccggccgcc gaacgcgatc gcggccaggt
     1141 cgatcacatc ccgggtggtg ggggccgaca cgccgccggc cacgtatggc ttgtcgctgg
     1201 accggatcac cgccaggtcc atctccagat gccggatggg cagcgggacg tcctcgggct
     1261 ccaccacgcc gctctgggac aggtgcagct gttccgatgc gtggaccacc cgcgtgaagg
     1321 tgaggtagtc cttcatggtg gccgggcgtc gccggccctc gccgtcgagc acgaacggcg
     1381 ctccccccgt gcaggtcagc accacgtggc gtccaccgaa cgtcacgtcc cgccgtcgat
     1441 cgcgtccccg gaccccgaac gtcgccggtg cagacgccag ccgttcgagc acgaaccccc
     1501 ggtcgaaccg gaccaggtcg tcctccgccc gcaggccggc ggctcgcagc acggccaggg
     1561 ccggctcgtg ggagatgcgc acccccacgt cctcgagcag agcgatcgcg tgccggtgca
     1621 ccgcttccac ctgctcggca tccagcaggt cgatccgcgg caagcggttc acccagccgg
     1681 tgggcacccg gggatcggta ccgacggtcc cttcgcgtga gggtgaggac atggcgaggt
     1741 ccactccggg gcggagccta tcccccgggt tcgggcctaa caaggagcgg ccctgcccgg
     1801 cggtcacccc tagcgatgcc ggtccaggat ggcgccggcc gcctgtccca ggtcgtccgc
     1861 ctccacgtcg gggagcgggt acgccgacag gtacgtggcc tccacgctcg agacccagac
     1921 cgtccgcagt ccggcgaagg tcgcaccggc gacgtcccag gcgtgcgccg ccaccagcca
     1981 gagctccccg cgcgcccgtg ccttggccat ctggtacacg cgcgggtccg gcttcgaggt
     2041 ccggatccga tcgcaggaca gcaggtccga gaacaggtcc aagatgccgg cgttccgcaa
     2101 catggcccgg gtggagcgct cactcccgtt cgtgagggcc agcaggcgca caccctcgtt
     2161 cgccaggcgc cgacaggcac cgggtgcatc cggtctcggg tccagctcgg agaaggcgga
     2221 catcaccgat cccagcccct cctcggaggc ccggacgccg gtggaggcga ccagccggcg
     2281 cagtcccgcc tccagcacgt cggccagcgg acggtagctg ccggcgtggg agagcgcgaa
     2341 cgcgtcacgg agcgactgcg cgaaccacag ctccagcgac cctgtgggca gcccgacctg
     2401 cgacagcgcc gcccgggggc gctccaggtc gaagagggtc ccgatcacgt cgaaggcgac
     2461 ggtggtcatg tgatccgcgc ccctgacggc acgctcctcg ctcatgctcc gcctatccat
     2521 gggtgcatgg ccttcggata cccgtaccga cctgggaaca accggtgcct gaggcggccg
     2581 gtatgctggc tagagggagc gcgcatgacc catccgaaca ggaccgtctt ccacgatcgg
     2641 cacgggcagc tcggagccag gttcatggac ttcggcgggt gggagatgcc cctgcagtac
     2701 cccaccggcg tggtgcagga acacctggcc acccgcaacc acgccgggct gttcgacgtc
     2761 tcgcacatgg ggcggttctg gatctccgga cccgggcggg tcgcgttcct ccagcacgtc
     2821 ctcacgaaca acgcggaggc gctggacggt cggcccaccg gggcccagta cacggtgatc
     2881 cccaccgagg ccggcggcgc gatggacgac gcctacctct accgcttcga cgaggagcgg
     2941 catttgctgg tggtgaacgc ctcgaaccga acgaaggatc tggagcacct ccgctcgcac
     3001 ctggggtcgt tcccccacgt cgagctgcgc gacgtcaccg aggagctcgt gatgctggcg
     3061 gtgcagggac cggccgcccg tgggctgctc tcggacctcg tggaggaagg gcggctgccc
     3121 gagccgatcc gcaacgccgt cgctgaggtc cggatcgccg gcgccggcgt gctggccggg
     3181 cgcaccgggt acacgggcga gccggtgggc ctggagctct tcctggaccg cgacgacggt
     3241 ccgaggctct gggacctgct ggtggagcgg ggggcgaccc cctgcggcct gggagcc
//
LOCUS       DASQQQ010000272        13246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_217833, whole genome shotgun sequence.
ACCESSION   DASQQQ010000272 DASQQQ010000000
VERSION     DASQQQ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13246
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217833"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VE646_10255"
     CDS             <1..600
                     /locus_tag="VE646_10255"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlpA-like double-psi beta-barrel
                     domain-containing protein"
                     /protein_id="HZD80404.1"
                     /translation="VRELLSAMGIRPDGDDRVHPSLQAPLHRHARVTFDKVTVITQRE
                     VVPIPFPTLTTYSTELPPGEVRVARIGRPGSLLEIFQVRRVNGVVGSKTLLSRSVLHA
                     AVPERRVVGRRASSVPVGNQVGQASWYDAPGSGLTAAHPSLPLGTVVTVTDLASGRSV
                     RVVINDRGPFGGRIIDLSPEAFSRLAPLGTGVLQVRLSW"
     gene            500..1489
                     /gene="rsmA"
                     /locus_tag="VE646_10260"
     CDS             500..1489
                     /gene="rsmA"
                     /locus_tag="VE646_10260"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZD80405.1"
                     /translation="MDRSAGASSTSRPKRSPGSPPSGPASSRSGSPGSGLGRSALKAL
                     ARRHGIRPSKTLGQNFLADPNMARAIVADAGVTASDRVVEVGAGLGSLTLPLASTGAQ
                     VVAVEFDRALADALSEVLAPHPNVRQVAGDALRLDWDELLEVGPSEGAGSSSERGWKM
                     VSNLPYNIAVPLLLDLLERVPAIRDLVVVIQREVGDRLVASAGDDAYGAVSLRVAYRA
                     RPELVRRISADVFWPRPNVGSVLVRLTPQPPPVDADPTLLFRVIDRSFAERRKTMTNA
                     LRRLGVGPDRAVRILRACSVPPRARPEDLDLDAYARVSDRLIEEGLVPEGADR"
     gene            1486..2358
                     /gene="ispE"
                     /locus_tag="VE646_10265"
     CDS             1486..2358
                     /gene="ispE"
                     /locus_tag="VE646_10265"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HZD80406.1"
                     /translation="MSPRRVVRREAHGKINVFLRVLGAREDGYHDLESLVLPVALADV
                     VTVRAARSLKVDVQGAAELAGSVPAGGLNLALVAALALAGVSGASRGATIRIDKRIPV
                     AGGLAGGSADAAATLSALNELWRCGLEEPTLADIAARVGSDVPAMLAGGPVLMHGRGE
                     RTAPATVSTLWWVMVTLGFGIRSPDAYRWWDEDGSPTGPDPQPLLDAAEAGDAGALGP
                     LLFNDLEPPVFARHREIARAKDSLLAAGALGAVMSGSGSSVGGLARDREHAERIAEFL
                     PGAIVTFGPPATSG"
     gene            2380..2450
                     /locus_tag="VE646_10270"
     tRNA            2380..2450
                     /locus_tag="VE646_10270"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2412..2414,aa:Gln,seq:ttg)
     gene            2548..3987
                     /gene="glmU"
                     /locus_tag="VE646_10275"
     CDS             2548..3987
                     /gene="glmU"
                     /locus_tag="VE646_10275"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HZD80407.1"
                     /translation="MPGRTPSRSLAAVVLAAGKGKRLRSDLPKVLHPVCGRPLLWHAL
                     QNALAARPARIVVVVSNGADQVVRAVRSWAIDPDPVFVDQGQPLGTGHAVLNAERAVG
                     RVDEVLVVGGDFDPVGPQDVRRLLRTHRRTGSAASILTTEVDEPRGYARVVRKGSRLL
                     EIVEGSDAPAELRDRREVSTLVFAFRRADLFRALPLVGRENRQHEYYLNEVFPILLAK
                     GERVSAVSADTGAMMGPNSRADLARLGELLRRRVAEAHMASGVGIVDPATTYIDVGVR
                     IGRDTVILPMTFLEGETRVGSGCRIGPGTRVIHSTVDDGAEVQFSVVRGSAVGPGAQV
                     GPYASLRPGTVLGEGAKAGTFVEIKKSRVGSGSKVPHLTYVGDAVIGRGTNIGAATVT
                     VNYDGYEKHGTVIGDDVRIGSDTMLVAPVEVGDRAVTGAGSVITKDVPPGALAVERSE
                     QRVVEGYRERKDAEARAKGKGKAVHEGEP"
     gene            3990..4970
                     /locus_tag="VE646_10280"
     CDS             3990..4970
                     /locus_tag="VE646_10280"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HZD80408.1"
                     /translation="MEIVTKKRMMLFSGTTHPALAAEIADHLGLSLAPCKVWRFASGE
                     IYARPEESVRGCDVFVIQSHADPVNESIMEQLVLLDAMKRASAKRITAVVPYYGYSRQ
                     DKKGLAREPISAKLVADLISVAGADRVVSVDLHSGQIQGYFDFPFDHLTALPVLSDYL
                     TNQLGLHDEDVVVVAPDAGRIKTAEKLREYLHADLAFMYKRRSRTEANKIEEMAVVGD
                     VEGRPCILVDDMIDTAGTVAKGATALAQQGGGPIYAAATHPVLSGKAAQNLEEAPIRE
                     VVVTNTLPIPEEKRFDKLKILSIAPLIGDALRAVFEDTSVSEIFRGDNQV"
     gene            5059..5748
                     /locus_tag="VE646_10285"
     CDS             5059..5748
                     /locus_tag="VE646_10285"
                     /inference="COORDINATES: protein motif:HMM:NF004133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HZD80409.1"
                     /translation="MAEQKLTAEKREDSGKGVARKLRAAGRVPAVLYGHGMQAMPLSV
                     DAKELFRILHTEAGANVLIDLLIDGQNHLALPREVQRDYVRDRLLHVDFLAVRRDVKV
                     AVDVRVQVVGESPGVKSGGVLEHQLWELHVECFPQDVPDAIEADISALEVGDSLKVAD
                     LKVSPDVTVLTAADETVLAVVTPQVRVVEEEVEEEALAEAEGATAEEAAEEAAEVAEP
                     GEAAAEEGGEG"
     gene            5785..6363
                     /gene="pth"
                     /locus_tag="VE646_10290"
     CDS             5785..6363
                     /gene="pth"
                     /locus_tag="VE646_10290"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020488132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HZD80410.1"
                     /translation="MTWIVAGLGNPGERFERTRHNLGRMVVDELARRAGARFRKVRFL
                     PLDLAEVHQAGQPVLLGKSQRFYNESGPVYASLARKREVDPGRLVAVHDDLDLAFGAM
                     RVKLGGSTAGNHGLDSLVGGLGTGDFHRVRLGIGRPPGRQDPVDFVLQPFPGRLEADV
                     AVLVDDAADAVLSLIGDGLGPTQDRFNRSGPR"
     gene            6430..9798
                     /gene="mfd"
                     /locus_tag="VE646_10295"
     CDS             6430..9798
                     /gene="mfd"
                     /locus_tag="VE646_10295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HZD80411.1"
                     /translation="MLERVLDALIASEPFERLLVERARPIVARAEAGGDVLLASLARA
                     LDSPLVAVAPGPREAEALAHGVRAFLGPRGAALFPAWEALPYEGISPSPETAARRAEA
                     ARALRRAHGPFVLVTPAHAALQGLVPTLGEREPLVLAEGQTFASDRLAQQLVELGYVR
                     SEVVEHRGEFAVRGGIVDVFPGTVRRPVRVELFGDDVESLREFVPATQLSTDAIARVE
                     VFPVRELTSTAELRERAEHLAPRFTDRFRDALTRFADGLWFEGMEALAPLLFDRLPTI
                     GDLLPHGCWVVLSQARRTMDRARQALDEAEQLAEASGWPGPPVLRPLDDVLQDRTRLH
                     LTEFAEGIDLGLEGWGTAQGNAPELAERLLDLAGRGYRVVVTAGAHGSLERAREVISE
                     RGLSLDGDARAAAWAVEADLAGGFVFPPGRLAVATEEDLFGTRRHTRSAPRITKRRAD
                     ALALELQPGDFAVHRVHGVGRFVGVQRRAVAGAERDYLVLEYAAGDRLSVPTDQVGMV
                     AKYLGGETPRLTRLGTNDWVRTTGRVKRAVKDMAGELVRLYSVRLSVEGHPFGPDAPW
                     QREMEDAFPHEETHDQASAIDEVKRDMERPRPMDRLICGDVGYGKTEIAVRAAFKAVM
                     DGKQVAVLVPTTLLAEQHFVTFSERFAPFPVRVAMLSRFLSQAEQQRVVEDVAAGRVD
                     VVVGTHRLLSKDLRFHDLGLLVVDEEQRFGVSHKERLKSLRAHVDVLTMTATPIPRTL
                     EMALTGIRDMSVVDTPPEDRQPVLTYVGPYAEDLALGAVRRELLRGGQIFWVHNRVAT
                     LDRQAAWIAEQVPEARIVVAHGQMDEELLEKQMLRFWDRDADVLVCTTIIESGLDVPS
                     ANTLVVDRADRLGLSQMYQLRGRVGRSTERAFSYFFFPPAQSLTEEAHERLSTISRHT
                     ALGSGFQIALRDLEIRGAGNILGAEQHGHIAAVGFDTYARLLRESVAEMKGEPLPEER
                     DIRVDLPVKAFIPVGWVGQEALRLELYRNIATAGDHERLSEVRAEAEDRFGQLPPEVS
                     TLLDVASLRITCERLGVQEVSTYRQQVRVKPFPLTEARELDLALTVPGASYHRTTGTL
                     NLVPERVAGADLPSWVERALRATGSGATSVTIAR"
     gene            9798..10820
                     /locus_tag="VE646_10300"
     CDS             9798..10820
                     /locus_tag="VE646_10300"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZD80412.1"
                     /translation="MQRFLRPLVPLVLAPLILTACASSRPAAATVDGQSITDTQLSQD
                     MVFFRFLSDLSQQSCGGPPVQGESAEASCARFTLGNLIQEDLVKGYAQSHHLSVPEAT
                     VTNALTQLESTVGGAKQLDSQLKAAGMTRADLTALARRLLLFDQVQKSVAAQRVSQQQ
                     LRQVYLQQITSFTQLDVRHILVKTEAQAQRIERQVNAKNFGDLAKKYSTDTQSAANGG
                     DLGTIAWSTFTQSYDADFVRATLKLKPGQISPPVRTQFGWHIIQLVSRKVQPFNAVED
                     QLRQSLGSQAFASWLQQRLADADISVNPKYGRLDRNSGEVVPVRTTATGSEAPTSPAP
                     AASATP"
     gene            10817..11635
                     /gene="mazG"
                     /locus_tag="VE646_10305"
     CDS             10817..11635
                     /gene="mazG"
                     /locus_tag="VE646_10305"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HZD80413.1"
                     /translation="MTTADPAHDPLAHVPSGPVPSSQQGERLLDLVRVMARLRGPGGC
                     PWDREQTHRSLARHLLEETHEVLEAIDADDRDRLREELGDVLLQVAFHAQMAADEGAW
                     DVDDVARGIVEKLIRRHPHVFGDVEVSGADEVLVNWERIKQEEKGPKGVDEDIPPTLP
                     SLARAAKVLRRAAGWGFAWRSLDGALDALKEEIDELEHHTSPRNAEEEIGDVLFATVA
                     VARTLGVDAESALRRTIRGFADRYERFTALVAARGLDLEAISEQEIRDLFRRAR"
     gene            11810..12886
                     /locus_tag="VE646_10310"
     CDS             11810..12886
                     /locus_tag="VE646_10310"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZD80414.1"
                     /translation="MTTVHAGPRSFRRGADPLSREALFRQVGSLVGAVVLGFLSVAMR
                     DATGAEGGILLRASTLTAVAVMAIMVIPWSRVPEAFHVLVPFAYLGAVYLVQSATGGP
                     YSSYGQLALLPVFWVAVFGRPRELGLVVLGAGVALGAPVVVAGGNVHDWARLATLLSV
                     GTGMALLVYRFFDGLRHQTTRLQVLAGTDPLTGAANRRAWDQELAGALIRATRDGRPV
                     CVALVDLDDFKGFNDSNGHQAGDRLLRQVAGAWQNQLRASDILARIGGDEFGILLPGC
                     PLDMAATIVRRLRDSVPAARCSIGVAEWDHREPVERLVARVDAALYEAKAAGRDRVVI
                     LPETGSTGVLPDRDAAGGPQGPDG"
     gene            12954..>13246
                     /gene="eno"
                     /locus_tag="VE646_10315"
     CDS             12954..>13246
                     /gene="eno"
                     /locus_tag="VE646_10315"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335557.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZD80415.1"
                     /translation="MPAIASLTARQILDSRGDPTVEVDAVLEDGAFGRASVPSGASTG
                     QHEALELRDGDPSRFGGKGVLRAVANVTDTIAEAVEGMEGLDQRGLDAALLEL"
BASE COUNT         1818 a         4540 c         4978 g         1910 t
ORIGIN      
        1 gttcgggaac tcctctcggc catggggatc cggcccgacg gtgacgatcg cgtccatcca
       61 tcactccagg ccccccttca ccggcacgcg cgggtcacct tcgacaaggt gaccgtgatc
      121 acccagcggg aggtcgtccc catcccgttc cccacgctca cgacgtactc gaccgaactg
      181 ccgcccgggg aggttcgggt ggcgaggatc ggccgacccg gctcactgct cgagatcttc
      241 caggtccgcc gcgtgaacgg cgtggtcggg tcaaagaccc tcctgtcccg cagcgtgctg
      301 cacgcggcgg tgccggagcg ccgggtggtg gggcggcggg ccagcagcgt ccccgtcggg
      361 aaccaggtcg gtcaggcatc gtggtacgac gcgcccgggt cggggctgac cgccgcgcac
      421 ccctcgctgc cattgggcac cgtggtgacg gtcacggatc tggccagcgg gcggtccgtt
      481 agggtggtga tcaacgaccg tggaccgttc ggcgggcgca tcatcgacct ctcgcccgaa
      541 gcgttctccc ggctcgcccc cctcgggacc ggcgtcctcc aggtccggct ctcctggtag
      601 cggcctgggg cgctcggccc tgaaggcgct cgcccgccgg cacggcatcc ggccctcgaa
      661 gaccctcggg cagaacttcc tggccgatcc caacatggcc cgggccatcg tggccgacgc
      721 cggcgtcacg gcctcggacc gggtggtcga ggtgggggcc ggactggggt cgctgacgct
      781 gccgctggca tccaccggcg cccaggtggt ggcggtcgag ttcgaccggg cgttggccga
      841 tgcgctgtcc gaggtgctcg cccctcaccc caacgttcgg caggtggccg gcgacgccct
      901 gcggctcgac tgggacgagc tgctcgaggt cggaccgtcc gagggagccg gatcctccag
      961 cgaacgggga tggaagatgg tctcgaacct cccctacaac atcgccgtcc cgctcctgct
     1021 cgacctgctg gagcgggtcc ccgcgatccg cgatctggtc gtggtgatcc agcgcgaggt
     1081 gggcgaccgg ctggtcgcct cggccgggga tgacgcctac ggggcggtga gccttcgcgt
     1141 ggcgtaccgg gcgcgcccgg agttggtgcg gcggatctcg gccgacgtct tctggccccg
     1201 gccgaacgtc gggtcggtcc tggtccgctt gaccccgcag ccgcccccgg tcgacgcgga
     1261 tcccacgctg ctgttccggg tgatcgaccg gtcgttcgcg gagcgacgca agaccatgac
     1321 caacgccctg cggcgactgg gtgtgggccc ggaccgagcg gtccggatcc tgcgggcgtg
     1381 ctcggtcccc ccgcgggccc ggcctgagga tctcgacctg gatgcctatg ctcgggtcag
     1441 cgatcgactg atcgaggagg gcttggtgcc ggagggggca gaccgatgag tccgcggcgc
     1501 gtggtgcgac gcgaggcgca cggcaagatc aacgtgttcc tgcgggtgct gggtgcccgg
     1561 gaggacggct accacgacct ggagagcctg gtgctgcccg tcgccctggc cgacgtggtg
     1621 acggtccggg cggcccgctc gctgaaggtg gacgtgcagg gggccgccga gctcgcggga
     1681 tcggtcccgg ccgggggcct gaacctcgcg ctggtcgcgg ccctggccct ggccggcgtg
     1741 agcggggcct cgaggggggc cacgatccgg atcgacaaac ggatcccggt ggccggaggc
     1801 ctcgccggtg gaagcgcgga tgccgccgca acgctctcgg ccctgaacga gttgtggcgg
     1861 tgcgggctgg aggagcccac gctggccgac atcgccgccc gggtgggttc cgacgtgccg
     1921 gccatgctgg ccggtgggcc ggtgctgatg cacggacgcg gtgagcggac cgcgccggcc
     1981 acggtgtcca ccctgtggtg ggtcatggtg acgttgggct tcggcatccg ctcgcccgat
     2041 gcctaccggt ggtgggacga ggatgggtcg cccaccggcc ccgaccccca accgctgctg
     2101 gacgcggcag aggcgggcga cgctggggcc ctcgggccgc tgctgttcaa cgacctcgag
     2161 ccgcccgtgt tcgcccgtca ccgggagatc gctcgggcca aggactcgct cctggccgcc
     2221 ggcgcgctgg gcgcggtgat gagcggcagc ggctcttcgg tcggtgggct ggctcgcgac
     2281 cgggagcatg cggagcgcat cgcggagttc ctgcccggag ccatcgtgac cttcgggccg
     2341 ccggcgactt cgggataatc gggggcgacc gagcatcgat ccggggtcgt ctaacggtaa
     2401 gacgcgggcc tttggagccc tcgatggagg ttcgactcct ccccccggaa cgctgccccg
     2461 gaaagcgcgg ctgtctcggg gcatccgccc ttgccacgtg atgcgtccgc ccggtagaac
     2521 agtgtcgccc tcgagcgaag gatcacggtg cccggaagaa ccccgtcccg ctccctggcc
     2581 gcggtggtgc tcgcggccgg gaagggcaag cggctgaggt cggatctgcc caaggtcctc
     2641 cacccggtct gtggtcgccc cctgttgtgg catgcgctcc agaacgcgct ggccgcccgg
     2701 ccggcgcgga tcgtcgtggt ggtctcgaac ggcgccgatc aggtggtgcg ggccgtgcgc
     2761 tcgtgggcca tcgatcccga ccccgtcttc gtggatcagg ggcagccgct cggcaccgga
     2821 cacgccgtgc tgaacgccga gcgggcggtg ggccgggtgg acgaggtgct ggtggtgggc
     2881 ggtgacttcg acccggtcgg cccgcaggac gtgcggcggc tgctgcgcac ccaccgccgg
     2941 accggctcgg ccgcctcgat cctgaccacc gaggtcgacg agccgcgggg ctacgcccgg
     3001 gtggtccgga agggctcacg gctgctcgag atcgtggaag ggagcgacgc tccggccgag
     3061 ctccgggacc ggcgcgaggt ctccacgctc gtgttcgcct tccgccgcgc cgacctgttc
     3121 cgagcgctgc cgctggtggg cagggagaac cggcagcacg agtactacct gaacgaggtc
     3181 ttccccatcc tgctggcgaa gggggagcgc gtctcggccg tctccgcgga caccggagcc
     3241 atgatgggac caaactcccg cgccgacctg gctcgcctgg gggagctgct ccgtcgtcgc
     3301 gtggcggagg cgcacatggc gagcggcgtc ggcatcgtgg atcccgccac cacctacatc
     3361 gatgtggggg tccggatcgg tcgggacacg gtgatcctgc cgatgacctt cctggaaggg
     3421 gagacccggg tgggatccgg ctgtcggatc ggaccgggca cccgggtgat ccactccacc
     3481 gtggacgacg gggccgaggt gcagttctcg gtggtgcgcg gctcggcggt cggccccggt
     3541 gcccaggtgg gcccgtacgc ctcgctgcgg ccgggcacgg tcttggggga gggggcgaag
     3601 gccggcacgt tcgtggagat caagaagtca cgggtggggt ccgggtcgaa ggtcccgcac
     3661 ctcacgtacg tgggcgacgc ggtgatcggc cgagggacga acatcggcgc cgccaccgtg
     3721 acggtcaact acgacgggta tgagaagcat ggaacggtga tcggagacga tgtgcggatc
     3781 ggttcggata ccatgctcgt cgccccggtg gaggtggggg atcgagccgt gacgggcgcg
     3841 ggatcggtga tcacgaagga cgtgccgccg ggcgcgctcg ccgtggagcg ctcggagcaa
     3901 cgggtggtcg aggggtaccg ggagcgcaag gacgccgagg cccgagccaa gggcaagggg
     3961 aaggccgtgc acgaggggga gccctgaccg tggagatcgt caccaagaag cggatgatgc
     4021 tgttctcggg gaccacgcac cccgcgctgg cggccgagat cgcggaccac ctcgggctga
     4081 gcctcgcgcc ctgcaaggtg tggcggttcg cgtcgggcga gatctatgcg cgccccgagg
     4141 agagcgtgcg cgggtgcgac gtgttcgtga tccagagcca cgccgacccg gtgaacgagt
     4201 cgatcatgga gcagctcgtc ctgctcgatg cgatgaagcg ggccagcgcg aaacgcatca
     4261 ccgcggtggt cccctactac gggtactcgc gccaggacaa gaaggggctg gcccgggagc
     4321 ccatctcggc caagctggtg gccgatctga tctcggtggc cggggccgat cgggtggtct
     4381 cggtggacct gcacagcggc cagatccagg gatacttcga cttccccttc gaccacttga
     4441 ccgcgctgcc ggtgctgtcc gactacctga ccaaccagct cggcctgcac gacgaggacg
     4501 tcgtcgtggt ggcgcccgac gccggccgga tcaagacggc cgagaagctc cgcgagtacc
     4561 tccacgccga cctggcgttc atgtacaagc ggcgttcgcg gaccgaggcg aacaagatcg
     4621 aggagatggc cgtggtcggg gacgtggagg ggcggccgtg catcctggtc gacgacatga
     4681 tcgacacggc ggggaccgtg gccaaggggg ccacggccct ggcccaacag ggaggcgggc
     4741 cgatctacgc ggcggccacg cacccggtgc tctccggcaa ggcggcccag aacctggagg
     4801 aggcgccgat ccgcgaggtc gtcgtcacga acaccctgcc catccccgag gagaagcgct
     4861 tcgacaagct gaagatcctg tcgatcgcgc ccctgatcgg cgatgcgctg cgcgcggtgt
     4921 tcgaggacac gagcgtctcc gagatcttcc gcggcgacaa ccaggtctag ggctcggccc
     4981 accgggcatc tccaccctcg atcgtcccgg tcgggacccg cgacccgttg gtacgatgcc
     5041 cggccggagg aggaacggat ggccgagcag aagctcacag ccgagaagcg ggaggacagc
     5101 ggcaagggcg tggcgcgcaa gctccgcgcc gccggccgcg tcccagccgt cctctacggt
     5161 cacgggatgc aggccatgcc gctgtcggtc gatgccaagg agctcttccg gatcctgcac
     5221 accgaggccg gggcgaacgt gctgatcgac ctgttgatcg acgggcagaa ccacctggcg
     5281 ctgccccgcg aggtacaacg cgactacgtc cgggatcgtc tcctgcacgt ggacttcttg
     5341 gccgtgcgtc gcgacgtcaa ggtcgcggtc gatgttcggg tccaggtggt cggcgagtcg
     5401 cccggtgtga agtcgggcgg cgtgctggag caccagctgt gggagctgca cgtcgagtgc
     5461 ttcccacagg acgtgcccga cgcgatcgag gccgacatct cggcgctcga ggtcggggac
     5521 tccctgaagg tcgccgacct gaaggtgtcg cccgacgtca ccgtgctcac ggcggccgac
     5581 gagaccgtgc tggccgtggt gaccccccag gtgcgcgtgg tggaggaaga ggtcgaggag
     5641 gaggccctgg ccgaggccga gggcgccacc gcggaggagg cggccgagga ggctgccgag
     5701 gtcgccgaac ccggcgaggc cgccgccgag gagggcggcg agggctagcc ggcggggacc
     5761 gggccggccg gagccgctcg ccccatgacc tggatcgtcg ccgggctggg caatcccggc
     5821 gaacgcttcg aacggacccg gcacaacctg gggcggatgg tcgtcgacga gctcgcccgc
     5881 cgcgccgggg cgcgcttccg caaggtgcgg ttcctgcccc tcgacctggc cgaggtccac
     5941 caggcgggcc agccggtgct gctggggaag tcgcaacgct tctacaacga gtccggtccg
     6001 gtgtacgcct cgctggccag gaagcgagag gtcgacccgg gccgcctggt ggccgtccac
     6061 gacgacctcg atctggcgtt cggggcgatg cgggtgaagc tcggcggctc cacggccggg
     6121 aaccatggac tcgattcgct ggtgggcggc ctgggcaccg gggacttcca ccgggtgcgc
     6181 ctcgggatcg gccgcccccc cgggcgccag gatccggtgg acttcgtgct ccagccgttc
     6241 cccgggcgcc tggaggcgga cgtggcggtc ctcgtggacg acgccgccga cgcggtgctg
     6301 tcgttgatcg gggatggcct cggtcccacg caggaccgct tcaatcgctc gggcccccgg
     6361 tagactggtc cagaaggccc cgtcgcagcg cgacgaggcc tcttcgcgct gtcccgcgga
     6421 ggtcggcagg tgctggaacg cgtgctggat gcgctgatcg cttcggagcc cttcgagcgg
     6481 ctcctggtgg agcgcgcccg ccccatcgtg gctcgggcgg aggccggcgg cgacgtcctg
     6541 ctggcctccc tggcccgcgc cctggactcg ccgctggtgg ccgtggcccc cggacccagg
     6601 gaagccgagg ccctggccca cggggtccga gcgttcctgg gcccgcgcgg ggcggcgctc
     6661 ttccccgcct gggaggcgct gccctacgag ggcatcagcc cgagcccaga gacggcggca
     6721 cgccgcgcgg aggcggctcg agcgctgcgt cgggcgcacg gtcccttcgt gctggtgacg
     6781 ccggcccacg ccgccctgca ggggctggtg ccgaccctcg gcgagcgcga accgctcgtg
     6841 ctggccgagg ggcagacctt cgcctcggac cgcctggcgc agcagctggt ggagctcggg
     6901 tacgtccgat cggaggtggt ggagcaccgg ggggagttcg cggtccgagg cgggatcgtc
     6961 gacgtgttcc ccgggaccgt ccgccgcccg gtccgggtgg agctgttcgg agacgacgtg
     7021 gagtccctgc gcgagttcgt cccggccacc cagctgtcca cggacgcgat cgcccgggtc
     7081 gaggtcttcc cggtgcgcga gctcacctcc acggcggagc tgcgggaacg agccgagcac
     7141 ctggcccccc ggttcaccga ccggttccgt gacgcgctca cgcgattcgc cgacggcctg
     7201 tggttcgagg gcatggaggc gctggcgccg ttgctgttcg accggctgcc caccatcggc
     7261 gacctgctgc ctcacggctg ttgggtggtg ctgtcccagg cgcgaaggac catggaccga
     7321 gcgcgccagg ccctcgacga ggccgagcag ctcgccgagg cctccgggtg gccgggcccg
     7381 ccggtgctgc gcccgctcga tgacgtcctc caggaccgga cccggctcca cctgaccgag
     7441 ttcgccgagg gcatcgacct gggcttggag ggctggggca cggcccaggg caacgcgccc
     7501 gagctggccg agcggctcct cgatctggcg gggcgggggt accgggtggt ggtgaccgcg
     7561 ggcgcccacg gcagcctgga gcgggcccgc gaggtgatct ccgagcgtgg gctgagcctc
     7621 gacggcgacg cccgggccgc ggcctgggcg gtggaggccg atctggccgg tgggttcgtg
     7681 ttcccgcccg gtcgtctggc cgtggccacc gaggaggatc tgttcgggac gcgccggcac
     7741 acgcgctcgg caccgcggat cacgaagcgt cgggccgacg ccctcgcgct cgagctgcag
     7801 ccgggcgact tcgcggtgca tcgggtccac ggcgtggggc ggttcgtggg ggtccagcgc
     7861 cgggccgtgg ccggcgccga gcgcgactac ctggtcctcg agtacgcggc gggcgatcgg
     7921 ctctcggtcc ccaccgacca ggtggggatg gtggccaagt acctgggcgg agagacgccc
     7981 cgcctgaccc ggctgggcac caacgactgg gtccgcacca ccggccgggt gaaacgcgcg
     8041 gtcaaggaca tggccggcga gctggtccgg ctgtactcgg tgcgcctgag cgtggagggg
     8101 caccccttcg gtccggacgc gccgtggcag cgggagatgg aggacgcctt cccacacgag
     8161 gagacccacg atcaggcctc ggccatcgat gaggtcaagc gggacatgga gcggcccagg
     8221 cccatggacc gcctgatctg cggcgacgtc ggctacggga agaccgagat cgccgttcgg
     8281 gccgccttca aggcggtgat ggacggcaag caggtggcgg tgctggtgcc caccacgctc
     8341 ctggccgagc agcatttcgt gaccttctcc gagcggttcg cccccttccc ggtcagggtg
     8401 gcgatgctgt ctcggttcct gtcccaggcc gagcagcagc gggtggtgga ggacgtcgcc
     8461 gccggccggg tggacgtggt ggtcggcacc caccggctcc tgtccaagga tctgcggttc
     8521 cacgatctgg gcctgctggt ggtggacgag gagcagcggt tcggcgtgtc ccacaaggaa
     8581 cggctgaaga gcctgcgcgc ccacgtggac gtgctcacga tgaccgccac gccgatcccg
     8641 cggaccctgg agatggccct caccgggatc cgggacatga gcgtggtgga cacgccgccc
     8701 gaggatcggc agccggtgct cacgtacgtg ggcccgtacg ccgaggacct ggccctgggg
     8761 gcggttcggc gggagctgct gcggggcggg cagatcttct gggtccacaa ccgggtcgcc
     8821 accctggacc ggcaggccgc ctggatcgcc gaacaggtgc cggaggcccg gatcgtcgtg
     8881 gcccacggac agatggacga ggagctgctc gagaagcaga tgctccggtt ctgggatcgc
     8941 gatgccgacg tgctggtctg caccacgatc atcgagtccg gtctggacgt gccgagcgcg
     9001 aacaccctgg tcgtggaccg ggccgaccgg ctcggcctct cgcagatgta ccagctgcgg
     9061 ggccgggtgg gacgctccac cgagcgggcg ttctcctact tcttcttccc gcccgcccag
     9121 agcctgaccg aggaggccca cgagcgcctg tccaccatca gccggcacac cgccctcggc
     9181 agcgggttcc agatcgccct gcgggacctc gagatccggg gggccggcaa catcctcggc
     9241 gccgagcagc acggacacat cgccgcggtg gggttcgaca cgtacgcccg gctcctccgc
     9301 gagtcggtgg ccgagatgaa gggcgagccc ctgccggagg agcgcgacat ccgggtggac
     9361 ctgccggtga aggcgttcat cccggtgggc tgggtgggtc aggaggcgct ccggctggag
     9421 ctgtaccgca acatcgccac ggccggcgat cacgagcggt tgtccgaggt ccgggccgaa
     9481 gccgaggacc ggttcggtca gctgcccccg gaggtgtcga ccctgctcga cgtggcgtcg
     9541 ctgcggatca cgtgcgagcg cctcggcgtg caggaggtct ccacctaccg gcagcaggtc
     9601 cgggtgaagc cgttcccgct caccgaggcc cgggagctgg acctggccct gaccgtgccg
     9661 ggggcgagct accaccgcac caccggaacc ctgaacctgg tgccggagcg ggtcgccggg
     9721 gccgacctcc cgagctgggt ggaacgcgcc ctccgggcca cgggatccgg cgccacctct
     9781 gttaccatcg cgcgctgatg caacgcttcc tccgcccgct ggtcccgttg gtgctcgccc
     9841 cgctgatcct gacggcctgc gcttcctccc ggccggccgc cgcaacggtg gacggccaga
     9901 gcatcaccga cacgcaactg tcccaggaca tggtgttctt ccggttcctg tcggatctgt
     9961 cgcagcagtc ctgcggcggc ccaccggtgc agggcgagtc cgcagaggcc tcgtgcgcgc
    10021 ggttcacgct cgggaacctg atccaggagg atctggtgaa gggctacgcc cagagccacc
    10081 acctctcggt ccccgaggcc accgtcacca acgcgctcac gcagctggag tccaccgtgg
    10141 gcggggcgaa gcagctcgac tcgcagctga aggccgcggg gatgacccga gcggacctca
    10201 ccgcgctggc tcgccggctc ctgctcttcg accaggtgca gaagtcggtg gccgcccagc
    10261 gcgtgtcgca gcaacagctc cggcaggtct acctgcagca gatcacgagc ttcacccagc
    10321 tcgatgtccg gcacatcctg gtcaagaccg aggctcaggc gcagcgcatc gagcggcagg
    10381 tgaacgcgaa gaacttcggg gacctggcga agaagtactc cacggacacc cagtcggccg
    10441 cgaacggcgg tgatctcggc accatcgcct ggtccacgtt cacccagagc tacgatgcgg
    10501 atttcgtccg ggccaccctg aagctcaagc cgggacagat cagcccaccg gtcaggacac
    10561 agttcgggtg gcacatcatc cagctggtga gccggaaggt ccagccgttc aacgcggtcg
    10621 aggaccagct ccgccagtcg ctggggtcgc aggcgttcgc cagctggctg cagcagcggc
    10681 tggcggacgc ggacatctcc gtgaacccga agtacgggcg cctggatcgg aactcgggcg
    10741 aagtggtgcc ggtgcggacc accgcgaccg ggtccgaggc gccgaccagc ccggcccccg
    10801 cggcctcggc cacgccgtga ccaccgccga tccggcccac gacccgctcg cgcacgtccc
    10861 gtccggcccg gtcccatcca gccagcaagg tgagcgcctg ctcgacctgg ttcgggtcat
    10921 ggcccggctg cgcggtcccg ggggatgtcc gtgggaccgc gagcagaccc accgttcgct
    10981 ggcccgacat ctcctggaag agacccatga ggtcctggag gcgatcgacg ccgacgaccg
    11041 tgatcggctc cgcgaggagc tcggtgacgt gctcctccag gtggctttcc acgcccagat
    11101 ggcggcggac gagggtgcct gggatgtcga tgacgtggcc cggggcatcg tggagaagct
    11161 gatccgccgg caccctcacg tgttcggcga cgtcgaggtc tcgggcgccg acgaggtgct
    11221 ggtcaactgg gagcggatca agcaggagga gaagggcccg aaaggcgtgg acgaggacat
    11281 cccgccgacg cttccttcgt tggctcgggc cgccaaggtc ctgcggcggg ccgccgggtg
    11341 gggcttcgcc tggcgttccc tcgacggcgc gttggacgcc ctgaaggagg agatcgacga
    11401 gctcgagcac cacaccagcc ctcggaacgc cgaggaggag atcggcgacg tcctgttcgc
    11461 cacggtggcg gtggcccgga ccctcggggt ggatgccgag agcgccctcc gccggaccat
    11521 cagggggttc gccgaccgct acgagcggtt cacggcgctc gtggcagcgc ggggtctgga
    11581 cctcgaggcc atctcggagc aggagatccg cgacctgttc cgccgggccc gctgagcgcg
    11641 gggcggacga agctgcatgg tcgatcggtc tcccccggac ggaggaacgc ccctgtgggc
    11701 cgcatgaggc cgtacgaatc cgtcgtccga gcgctcatgc ttgggcgggc gatgcgccga
    11761 taacccccgt gagcggtccc gtgacgagcg gtgactggcc agggaccgca tgaccacggt
    11821 gcacgcaggg cctcgatcct tccgacgcgg tgcggacccc ctgtcgcgtg aagcgctgtt
    11881 ccgccaggtg gggagcctgg tcggcgcggt cgtgctgggg ttcctgtccg tggccatgcg
    11941 cgacgccacc ggggcggagg gggggatcct gctgcgggcc tccaccctga ccgcggtcgc
    12001 cgtgatggcc atcatggtga tcccgtggag ccgggtcccc gaggcgttcc acgtcctggt
    12061 ccccttcgcc tacctcggcg ccgtctacct ggtgcagagc gcgaccggcg gaccgtactc
    12121 ctcctacggg cagctggccc tgctcccggt gttctgggtg gccgtgttcg gccggccccg
    12181 cgagctgggc ctggtggtgc tcggcgcggg cgtggcgctg ggggctccgg tggtggtggc
    12241 cggcgggaac gtgcacgact gggcccggct cgcgaccctg ctgtcggtcg gcacggggat
    12301 ggcccttctc gtgtaccgct tcttcgatgg tctgcgccac cagaccaccc gcctgcaggt
    12361 gctggccgga accgatcccc tgaccggcgc ggccaaccgg cgggcctggg accaggagct
    12421 ggccggcgcg ctgatccggg ccacccggga cggccgcccg gtctgcgtgg ccctggtcga
    12481 cctggacgac ttcaagggct tcaacgactc gaacggccac caggcggggg accggttgct
    12541 gaggcaggtc gccggagcct ggcagaacca gctgcgggcc agcgacatcc tggcccggat
    12601 cggcggtgac gagttcggga tcctcctgcc cgggtgcccg ctcgacatgg ccgccaccat
    12661 cgtgcggcgg ctgcgggact ccgtgcccgc ggcccggtgc tcgatcggcg tggcggagtg
    12721 ggaccaccgg gagccggtgg agcggctggt ggccagggtc gacgccgccc tgtacgaggc
    12781 caaggcggcg gggcgtgacc gcgtggtgat cctccccgag acggggagca cgggggtgct
    12841 tcccgatcgg gacgcggccg ggggaccgca gggaccggac gggtaggccg accgcgggag
    12901 gtccatcgcc atctcccccg atcgcagggt gcgggttgta cgatcgaccc cgcatgcctg
    12961 cgatcgcctc gctcaccgcg cgccagatcc tggattcgcg cggcgacccc accgtggagg
    13021 tggatgccgt cctcgaggac ggcgcgttcg gacgcgcctc cgtcccgtcc ggagcctcca
    13081 ccgggcagca cgaggctctc gagctccgtg acggggaccc ctcgcggttc ggtggcaagg
    13141 gcgtcctgcg ggcggtggcg aacgtgaccg acaccatcgc cgaggcggtc gaggggatgg
    13201 agggtctcga ccagcgcggg ctcgatgcgg ccctcctgga gctgga
//
LOCUS       DASQQQ010000273         2504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_161144, whole genome shotgun sequence.
ACCESSION   DASQQQ010000273 DASQQQ010000000
VERSION     DASQQQ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161144"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VE646_10320"
     CDS             complement(<1..597)
                     /locus_tag="VE646_10320"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HZD80416.1"
                     /translation="MVERIVDLLRPYLSVPWGYVIVGVATLLENSVGAGVIVPGETLV
                     IVGGFYARIGNLDLAWVAVAACLGAVVGDNVGFLIGRRFGRGFLERHGRKVLVTPQRL
                     DRADRYYQAHGGKTVFLGRFIPVVRSVGFILAGVARMPWRRFFLYDLAGAVLWGVGHS
                     ILGYAIGASYERWKRFLTPAGLAILLVLLLLIGGSKLVA"
     gene            651..1427
                     /locus_tag="VE646_10325"
     CDS             651..1427
                     /locus_tag="VE646_10325"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZD80417.1"
                     /translation="MTPLRAAAAVLVGLVAGVLSGLFGVGGSIVTTPAINVLLGGTAI
                     QAVGTPLPVTFPTALAGAVAYARARQLSWRAFRWAAIPGIAGAVGGAWLTDFVNPHIL
                     LVITAVLIGWTAVRVVRGRPARVSWELGKTQGWIYGMVGLVAGFVSGLLGVGGGIIFV
                     PAFALLIGMPLKRSLGTSLVTITAIVIPGTVVHGLLGHIDWAIALFLTVGVIPGASLG
                     ARIALRTRDRTLRGAVGAFLMTIAVLYGIREVAALLAGRG"
     gene            1444..>2504
                     /locus_tag="VE646_10330"
     CDS             1444..>2504
                     /locus_tag="VE646_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80418.1"
                     /translation="MHRLAALLLVWVAVLLVPLPTASAQDAGAIRLTLVSQTPWNTIR
                     DQVLEVRVRAQNTGDAPVKDLSLGLTMFGPLFSRTAYETSLTQDPPSAVIAFQTFTPQ
                     GDLPAGGSRVFRVALDLSTAAVSRTQSLVYPLRVELRSGFTTLAALRTPVIFLVRRPE
                     TPLSLAWTFVLTHPIDLRPDGVFRSPDLEQQLTRGSRLAGELRALTGLVQGRSSAPVD
                     VVVGPMLVSQLAMMASGYRVVHGASVRQVAKGTDGSALAAEALSNLRTIGGAPQVELS
                     ATPYSEAQVPSLESGGLARDLAAQVTRGREVLSSTLSGTPDPSILRPPDSAVDPGSLE
                     ALPPQGVSLLLLDPGAAPQA"
BASE COUNT          323 a          924 c          884 g          373 t
ORIGIN      
        1 ggcgaccagc tttgagcccc cgatcagcag cagcaggacc agcaggatgg ccaaaccggc
       61 cggcgtcagg aagcgcttcc agcgttcgta gctggcaccg atcgcgtacc cgaggatcga
      121 gtgcccgacg ccccacagga ccgcgcctgc caggtcgtac aggaagaacc gtcgccaggg
      181 catcctcgcc accccggcca ggatgaaccc cacgctgcgg acgacgggga tgaaccggcc
      241 caggaacacc gtcttgcccc cgtgcgcctg gtagtagcgg tcggcccggt ccaggcgttg
      301 gggggtcacc agcaccttgc gtccgtggcg ctcgaggaag ccgcggccga accgccggcc
      361 gatcaggaaa ccgacgttgt cgccgacgac cgccccgagg cacgccgcga ccgccaccca
      421 ggccaggtcc aggttcccga tccgcgcata gaatccgccg acgatgacca gcgtctcgcc
      481 gggcacgatc accccggccc ccaccgagtt ctccaggagt gtggcgacgc cgacgatcac
      541 gtatccccag ggcaccgaca ggtacggacg caggagatcg acgatgcgct cgaccactgg
      601 aggcaagtat aggttggggc cgttgcccga gtgcccgtga cggcgcgcag atgaccccgc
      661 tccgggcggc ggcggccgtc ctggtcggac tggtggccgg ggtgctctcc gggctgttcg
      721 gcgtgggggg gagcatcgtc accacgccgg ccatcaacgt gctcctgggc gggacggcga
      781 tccaggccgt ggggacgccg ctgccggtga cgttccccac cgctctggcg ggggccgtgg
      841 cctacgctcg ggcacgacag ctcagctggc gggcgttccg atgggccgcc atcccgggga
      901 tcgcgggcgc ggtcgggggg gcctggctca ccgacttcgt caacccgcac atcctgctgg
      961 tgatcacggc cgtcctgatc ggctggaccg cggtgcgggt ggtgcgtgga cggcccgctc
     1021 gggtgtcctg ggagctcggc aagacccagg ggtggatcta cgggatggtg gggttggtgg
     1081 ccgggttcgt gtccgggctg ctgggggtgg gcggagggat catcttcgtc ccggccttcg
     1141 ccctgctcat cggcatgccg ctgaagcgct ccctggggac gtcgctggtg acgatcaccg
     1201 cgatcgtgat ccccggcacg gtggtccacg gcctgctcgg gcacatcgac tgggccatcg
     1261 cgctgttcct cacggtcggc gtgatccccg gagccagcct gggcgcgagg atcgccctgc
     1321 ggacccgcga ccgcacgctg cggggggcgg tgggggcctt cctcatgacg atcgcggtcc
     1381 tctacggcat ccgcgaggtc gcggccctgc tggcgggccg cgggtaggat gggtcggtcg
     1441 acgatgcacc ggctcgccgc actcctcctg gtctgggtcg ccgtgctcct ggtgcccctc
     1501 cccaccgcct ccgcgcagga cgcgggggcc atccgactga ccctcgtctc ccagacgccg
     1561 tggaacacca tccgcgacca ggtgctcgag gttcgcgtgc gggcgcagaa cacgggcgac
     1621 gcaccggtca aggacctctc cctgggcctc acgatgttcg ggccgctctt cagccggacc
     1681 gcgtacgaga cctccctgac ccaggatccg cccagcgccg tgatcgcctt ccagacgttc
     1741 accccgcagg gcgacctccc ggcgggcgga tctcgcgtct tccgcgtggc cctggacctc
     1801 tccaccgcgg ccgtgtcgcg gacccagtcc ctggtgtacc cgctgcgcgt cgagctccgc
     1861 agcggcttca ccacgctggc ggccctgcgg accccggtga tcttcctggt ccgccgcccc
     1921 gagaccccct tgtcgctcgc ctggaccttc gtcctgaccc acccgatcga tctgcggccc
     1981 gacggcgtct tccgctcgcc ggacctggaa cagcagctga ctcggggcag tcggctggcc
     2041 ggcgagctgc gagcgctgac cggcctggtg cagggccgtt cctcggcccc ggtcgacgtg
     2101 gtggtggggc ccatgctcgt gagccagctg gcgatgatgg cctcgggcta ccgggtggtc
     2161 cacggggcca gcgtgcgcca ggtcgcgaag gggaccgacg gatcggccct cgcggccgag
     2221 gccctgtcga acctgcgcac gatcgggggc gcgccccagg tggagctctc ggccacgccg
     2281 tactccgagg cccaggtccc gtccctcgag tccggaggac tggcccgcga cctggccgcc
     2341 caggtgaccc ggggacgcga ggtcctctcc agcaccctgt ccggcacgcc ggatccgtcg
     2401 atcctccgtc ctccggactc ggccgtggat ccggggagcc tggaggcgct tcccccccag
     2461 ggcgtctccc tcctgctgct ggatcccggg gcggcccctc aggc
//
LOCUS       DASQQQ010000274         2540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_348187, whole genome shotgun sequence.
ACCESSION   DASQQQ010000274 DASQQQ010000000
VERSION     DASQQQ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348187"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..117
                     /locus_tag="VE646_10335"
     CDS             13..117
                     /locus_tag="VE646_10335"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HZD80419.1"
                     /translation="MRPRQWYAAGARLIGGCCGTGPEDIRGIATALGR"
     gene            complement(125..910)
                     /locus_tag="VE646_10340"
     CDS             complement(125..910)
                     /locus_tag="VE646_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZD80420.1"
                     /translation="MGSLDQLGVAAAAVAGGAINALAGGGTLITFPTLTAVGIPAVRA
                     NVTNTVALSPGYLGGTLAQRRDLHGQARRMWLVVPAGVLGGLTGGVALLLTSEGLFRA
                     LVPYLILLASLLLAVQERLRALVSRRVSAGMRGSTGTIGERSIREAWVVVPAFAASVY
                     GGYFGAGLGVILLAVLGLVIDDTLTRINALKQAVSFAVNITAALFFLFSGKVVWTVAL
                     TMAIGAVVGGVLGGRLAGRISPSALRRVVVAIGVIVAVIYLVR"
     gene            1157..1414
                     /locus_tag="VE646_10345"
     CDS             1157..1414
                     /locus_tag="VE646_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80421.1"
                     /translation="MWSPTVEQFEEHYQLSCRRCGHRWPLVYTVRRQMEPDGDSQEVY
                     FRRGLPASSPWAGRPCPRCADQRVDVRMTHRRPLPPLRSAG"
     gene            1473..2249
                     /locus_tag="VE646_10350"
     CDS             1473..2249
                     /locus_tag="VE646_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZD80422.1"
                     /translation="MEAVTVVNLDGAVCLVTGASSGIGRALAPRLSVAGAKVVVLGRD
                     GPALQGVAARSGGTVVVADLAREGAPAEAADQALRAHGRVDVLVNNAGIGLTGALLDT
                     DPDAAEHALTVDLIAPVLLTRALLPGMRERGRGRVVNVASIAGHVGVPEEAVYAAAKA
                     GLVIFSESLRQELRGTGVGVTVISPGAVRTAFHEREGRPYDRRFPTLLSADRVARVTV
                     DALAHDRERVFVPRWMAFPAWLHGAAPRLYERLAERYGSR"
     gene            complement(2262..>2540)
                     /locus_tag="VE646_10355"
     CDS             complement(2262..>2540)
                     /locus_tag="VE646_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3463 domain-containing protein"
                     /protein_id="HZD80423.1"
                     /translation="LDFLEGKVDYECTPWGIPCYTIFGWQRPCYLQSDGYCDTYQELV
                     ETTDWSNYGRHSDNVQCRNCMAHCGYEPTAVMATTHSLKESMRAVVGG"
BASE COUNT          369 a          934 c          897 g          340 t
ORIGIN      
        1 gcgggtccgg gcctgcgtcc gcgtcagtgg tacgcggcag gagcgcgcct gatcgggggc
       61 tgctgcggca ccggccccga ggacatccgg gggatcgcca ccgcgctggg gcggtgaccg
      121 gctctcagcg cacgaggtag atcacggcca cgatcacccc gatcgccacc accacgcgcc
      181 gaagggccga cgggctgatg cggccggcca ggcgcccccc cagcacgcct cccacaaccg
      241 ctccgatcgc catggtgagg gccaccgtcc agaccacctt gcccgagaag aggaagaaca
      301 gggccgccgt gatgttcacc gcgaaggaca cggcctgctt gagggcgttg atcctggtca
      361 gcgtgtcgtc gatcaccagg ccgagcacgg ccagcaggat cacgccgagg ccggccccga
      421 agtaccctcc gtagacggac gcggcgaacg ccggcaccac cacccaggcc tcccggatcg
      481 atcgttctcc gatcgttccg gtcgatccgc gcatgccggc gctcacccgg cgcgagacca
      541 acgcgcgcag ccgctcctgc acggccagca gcagcgacgc cagcaggatc aggtacggca
      601 ccagggcgcg gaagagtccc tccgaggtca gcaggagcgc caccccgccc gtcaggccac
      661 ccaggacgcc ggccggcacc acgagccaca tccgccgcgc ctggccatgg aggtcccgtc
      721 gctgcgcgag cgtcccgccc aggtagccgg gcgacagggc gacggtgttg gtgacgttgg
      781 cccgcaccgc ggggatcccg accgcggtga gggtggggaa cgtgatcagc gtccctccgc
      841 ccgcgagggc gttgatcgcg ccccccgcga cggctgcggc ggccaccccc agctgatcga
      901 gggacccgat cgtcacgcca acgcctctcc tccgccgccc gcgagcatcg cggcgtcctg
      961 tgccaacggt ccggaccata caggctgggg acggtcccgg ctcggagcac cgctcctcat
     1021 cgcccccctt gccgcaacac gatcgccacg aacgatccgc tccccaactg aaccggtgcg
     1081 gggaccagag acgaagcaca ggtgtggggg acgatccacc gtacccccgg agccatccga
     1141 ggagatgatg ccgtccgtgt ggagtccgac cgtcgagcag ttcgaggagc actatcagtt
     1201 gtcctgtcgc cgctgcgggc atcgctggcc gttggtgtac acggtccgcc ggcagatgga
     1261 gcccgacggc gacagtcagg aggtctactt ccggcggggt ctgcccgcca gctcgccctg
     1321 ggcggggcgt ccctgccctc gatgcgccga tcaacgcgtg gatgttcgga tgacgcaccg
     1381 acggccgctc ccaccgctgc gctcggccgg ctgagcttcg ccggccgagc tccgggcggc
     1441 ccgacgatgg tagcgtccgc agcaaccgcc gcatggaggc cgtgaccgtc gtgaacctgg
     1501 atggagccgt gtgcctggtc acgggcgctt ccagcggcat cggacgcgcc ctggccccgc
     1561 ggctgtccgt cgccggcgcc aaggtggtgg tcctgggccg ggacggcccg gccctccaag
     1621 gcgtcgcggc ccggtccggt gggacggtgg tggtggcgga tctggctcgg gagggggctc
     1681 cggccgaggc ggcggatcag gcgctccggg cccacgggcg tgtcgacgtc ctcgtgaaca
     1741 acgcggggat tgggctgacc ggggcgctcc tggacaccga ccccgacgcg gccgagcacg
     1801 cgctcacggt cgacctgatc gctccggtgc tcctcacccg ggccctcctg ccgggcatgc
     1861 gagagcgggg acggggacgg gtggtgaacg tcgcctcgat cgctgggcac gtgggcgtgc
     1921 ccgaggaagc cgtctacgcg gccgcgaagg ccgggctggt gatcttcagc gagagcctgc
     1981 gccaggagct ccggggcacc ggtgtcggcg tcaccgtgat ctcaccgggc gccgtacgga
     2041 cggccttcca cgagcgcgag ggccgaccct acgaccggcg gttccccacg ctcctgtccg
     2101 ccgaccgggt cgctcgggtc acggtcgacg cgctggccca cgaccgcgag cgggtcttcg
     2161 tcccccgatg gatggccttc cccgcatggc tgcacggcgc cgccccccgc ctgtacgagc
     2221 ggctggcgga gcgctacggc tcccggtgag ccggagccgg gtcagccccc caccaccgcc
     2281 cgcatcgact ccttgaggga gtgcgtggtg gccatcacgg ccgtcggctc gtaaccgcag
     2341 tgcgccatgc agttgcggca ctggacgttg tcgctgtgcc gaccgtagtt ggaccagtcg
     2401 gtggtctcca ccagctcctg gtacgtgtcg cagtacccgt ccgactggag atagcagggg
     2461 cgctgccacc cgaagatcgt gtagcaaggg atgccccacg gggtgcactc gtagtcgacc
     2521 ttgccctcca ggaagtccag
//
LOCUS       DASQQQ010000275         9861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_266757, whole genome shotgun sequence.
ACCESSION   DASQQQ010000275 DASQQQ010000000
VERSION     DASQQQ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9861
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266757"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VE646_10360"
     CDS             <1..391
                     /locus_tag="VE646_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase hydrophobic membrane
                     anchor subunit"
                     /protein_id="HZD80424.1"
                     /translation="YRTGRERPAGGWELWTWLFMRISGVVLLWLAVGHVLIMHVLGGG
                     VGRVDFAFVAARWQSPFWRTWDWALLSLALLHGINGLRVVVQDYVRPVAWRFAVNMFF
                     YVLGFLLFVLGTVIVFTFDPSRWPGVS"
     gene            399..2129
                     /gene="sdhA"
                     /locus_tag="VE646_10365"
     CDS             399..2129
                     /gene="sdhA"
                     /locus_tag="VE646_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006127025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HZD80425.1"
                     /translation="MIHEYECVIVGAGGAGMRAAIEASDRTRTAVISKLYPTRSHTGT
                     AQGGVCAALGNVEEDSPEWHAFDTVKGGDYLVDQPAAQLMCEEAIHAIYELEHWGLPF
                     NRTPQGRIDQRRFGGHTRNHGEAPVRRACYAADRTGHMILQTLYQQCVKRGVRFFNEF
                     YVLDLLMNEGRTAGVVAYELATGELHVFRATSVLFATGGSGRMFKVSSNAHTLTGDGQ
                     AVVYRRGVPLEDMEFFQFHPTGVYKMGILLSEAVRGEGGILLNSEGERFMERYAPTVK
                     DLAPRDLVSRAIFQEVQEGRGIGPGKDGVYLDVRHLPRDVIEEKLPDVTEFARIYLGV
                     EPTTEPVPIQPTAHYTMGGIPTDVDGVVVTGEDETPVPGLYAAGECACVSVHGANRLG
                     TNSLLDIVVFGRRGGRQMAEFAREADPVEPPEEAEAFVRGMLGELLARSSGENTADIR
                     SELQDSMYDLAFVVRTEQSLRKMAEILKGLQERFDRAPVQDKGSRYNTDLMETVELGF
                     LLDNAEALVASALAREESRGAHYREDHPIRDDDHWLKHTLATREADGSVRLDYKPVQL
                     GPYVPMERKY"
     gene            2129..2902
                     /locus_tag="VE646_10370"
     CDS             2129..2902
                     /locus_tag="VE646_10370"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HZD80426.1"
                     /translation="MAAGEQLGDVLDASEERRAGASGKVSLTLKIRRYNPELRGEESW
                     WDEFTIEAHPNDRLLDALHEVKWHHDGTLAVRRSCAHGICGSDAMMVNGKNRLACKVL
                     VREVVPHVTVEPIRGLPVLKDLYVDMEPFFAGYRSIIPYLVNDEGEPERERLQSPEER
                     HRFDDTTKCILCGICTTSCPIFWGDEAYVGPAAIVNAHRFIYDSRDRAGSERLKILSE
                     KTGVFRCRTTFNCTEACPRGIEVTKAIQDVKRSILFDRF"
     gene            2989..4104
                     /locus_tag="VE646_10375"
     CDS             2989..4104
                     /locus_tag="VE646_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015164042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HZD80427.1"
                     /translation="MAHLVTLIPGDGIGPEVAQAARRVLDATGVGFEWVVEQAGAAVL
                     EREGTTLPDRVLEQIRRSRVAIKGPITTPVGRGFRSVNVALRHDLDLFAAVRPSRSLP
                     GVPTRYPDVDLVVIRENTEDLYAGIEFEAGAPETDRLRARLHQLAGADLRQDAGITLK
                     PISVSGARRIVRFAFEHARANGRRRVTLGHKANIMLFSDGLFLDIARREAPAYADVGF
                     EEMQIDQLSMRLTRSPADFDVLLLPNLYGDILSDLCAGLVGGLGLAAGANLGWEYAVF
                     EPVHGSAPDIAGRGLANPVAMILSGVMMLRHLGETAAADAVRSAVDRVLLEGEARTRD
                     LGGSASTEEVATAVTKALSAPGRSGWSDGPQRARNQG"
     gene            4125..4325
                     /locus_tag="VE646_10380"
     CDS             4125..4325
                     /locus_tag="VE646_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1918 domain-containing protein"
                     /protein_id="HZD80428.1"
                     /translation="MTAKVGDRIVVESETVGQPIREGEILEVLEGEVGIRYRVRWEDG
                     HETIFTPSGGSARIVSAESARR"
     gene            complement(4398..4556)
                     /locus_tag="VE646_10385"
     CDS             complement(4398..4556)
                     /locus_tag="VE646_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80429.1"
                     /translation="MANARRTPHAFFTNWRSYQGPLGTKLWLTARNRFRAVVLLKGCC
                     GHDGEPGC"
     gene            complement(4600..5214)
                     /locus_tag="VE646_10390"
     CDS             complement(4600..5214)
                     /locus_tag="VE646_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HZD80430.1"
                     /translation="MSLELLDDGRKRILAPLSDQDVRRLTAGDVVLVSGTIYAARDAA
                     HKRLVEMLDAGQELPFDPSGQIVYYVGPTPERPGKAIGSAGPTTASRMDGYTPRLLEI
                     GLKGIIGKGGRGPIVREALRRHTAVYLAALGGGGALAALRISSQRVIAFPELGPEAIR
                     EMRVEGFPVWVVNDAEGRDFYSQTIRPWRRNELLPESLRLPTNV"
     gene            complement(5217..6080)
                     /locus_tag="VE646_10395"
     CDS             complement(5217..6080)
                     /locus_tag="VE646_10395"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HZD80431.1"
                     /translation="MAASAPVREIDAAQITDAVRDLCVRVNYRIPEDVVEAVASARRR
                     EESPLGRRVLDLIAENQRLAAEGEYPYCQDTGSTVVFIDVGQDVHVVGADLEDAINEG
                     VRRGYREGYLRTSIVRDPVFERTNTGDNTPAFIHTRIVPGDQVRIQVDAKGGGSENKG
                     RHAMLKPSDGLEGVTTFVLTTVAEAGPDACPPGIVGVGIGGNFEYAAILAKRALLRKV
                     GLPSPDPRVAELEADLLEGCNALGIGPQSLGGTQTVLAVHVETMPTHIASLPVAVNLE
                     CHAHRTGTTVI"
     gene            6145..6420
                     /locus_tag="VE646_10400"
     CDS             6145..6420
                     /locus_tag="VE646_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80432.1"
                     /translation="MGPELLSTARPTRLRLAGFLALEWWGRLVARGSLRTWATVRPFR
                     TGTAGIDLWEGELTLALGLSVLVGVIVMRMLAAPGPDATGARSEPGP"
     gene            complement(6428..7351)
                     /gene="mdh"
                     /locus_tag="VE646_10405"
     CDS             complement(6428..7351)
                     /gene="mdh"
                     /locus_tag="VE646_10405"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZD80433.1"
                     /translation="MKVTVVGSGFVGQTTAMRIVEKGLAEVVLIDIIEGLPQGLALDM
                     SEAAPVEGYEPVITGTNDYAETAGSDVVVITAGFPRQPGMSRMDLLGKNAGVIRQVVE
                     NVRDGSPDAIIVMVTNPLDEMTFLASEVSGFPKQRVIGMAGVLDSSRLRYFIAEELGV
                     SPSDVEAMTLGSHGDAMVPLPRHATVGGKPLTELVDESTLERLYQRTRDAGAEIVGLL
                     KKGSAYYAPSASITKMVASIVHDSEEMLPVCAWCTGQYGIDGVYVGVPVRLSSSGVEE
                     VVELDLSQDELGALRAAAEGIRSKCEDLSKL"
     gene            complement(7428..8510)
                     /locus_tag="VE646_10410"
     CDS             complement(7428..8510)
                     /locus_tag="VE646_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HZD80434.1"
                     /translation="MAYRVTFIPGDGVGPELSEATRRVLEGTGVAFDWDLQDAGADVM
                     ERYGTPLPEPVLASIRDTKVAIKGPITTPVGSGFRSVNVALRKELDLYSCLRPCKSYR
                     GVRSRYQDVDIVIVRENHEDLYAGIEFEQGTPEAKELIEFVAGATGKRIRDDAGISIK
                     PISVWGTERIVRFAFEHARRYGRHKVTAVHKANIMKFSDGLFLDTARQVAAGYPDIEF
                     EDRIVDNLTMQLVQRPEEYDVLVLPNLYGDIVSDLCAGLVGGLGLAPGANIGDEAAVF
                     EATHGSAPKYKGMNKVNPMAMMLSGVLMLRHLGEPGAADRLEAAIAAVIEEGRSVTYD
                     MKPTRDDPTAVGTSQVADAVIERLQG"
     gene            8655..9773
                     /locus_tag="VE646_10415"
     CDS             8655..9773
                     /locus_tag="VE646_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HZD80435.1"
                     /translation="MADKGLEEVIAASTRLSDVDGKAGRLWYVGFGIEDLAERSTFEE
                     VVYLLHNLRLPTEPELDDLHERLVSEREVSTFLADLMPTLAQQTSPMSMLRTSVSAAS
                     AYDPDGWEQTPAANERKALRLIARIPTMIAMYHRLRTDQEVVLPNPKLPHAANFLWML
                     LGEEPDQEDAAVLDTTLILYADHTMNASTFTARVIASTLADMHSAITGAMAALKGPLH
                     GGANEEAMKLLEEVGDPANAERVVKEKLARKERIMGFGHRVYKSGDPRAAVLRRLAKQ
                     TGERKRDPRWYEISEEIERVVGEEKGLHANVDFYAASVYHYLGIPHDLMTPVFALART
                     SGWTAHVREQYGDNRLIRPESEYIGPRDQVYVPIDQRG"
BASE COUNT         1469 a         3469 c         3418 g         1505 t
ORIGIN      
        1 gtaccgcacc ggtagggagc gccccgccgg cgggtgggag ctgtggacct ggctgttcat
       61 gcggatcagc ggagtggtgc tcctgtggct ggcggtgggc cacgtcctga tcatgcacgt
      121 gttgggtggc ggtgtggggc gcgtggactt cgcgttcgtc gccgcccggt ggcagagccc
      181 cttctggcgg acctgggact gggcgctgct gtcgctcgcg ctgctgcatg ggatcaacgg
      241 gctgcgggtg gtggtgcagg actacgttcg gccggtggcc tggcggttcg ccgtgaacat
      301 gttcttctac gtgctgggct tcctgctgtt cgtcctcggt accgtgatcg tgttcacgtt
      361 cgatccgagc aggtggccgg gggtgtcctg acgtggccgt gatccacgag tacgagtgcg
      421 tgatcgtggg cgcgggcggg gccgggatgc gcgcggccat cgaggcctcg gaccggacac
      481 gcaccgcggt gatctcgaag ctctacccca cccgttccca caccgggacg gcgcagggtg
      541 gcgtctgtgc cgcgctcggc aacgtggagg aggattcccc ggagtggcac gccttcgaca
      601 cggtgaaggg cggggactac ctggtggacc agcccgcggc ccagctgatg tgcgaggaag
      661 cgatccacgc gatctacgag ctggagcact gggggctgcc gttcaacagg acgccccaag
      721 ggcggatcga ccagcggcgg ttcggcggtc atacccgcaa ccacggcgag gcaccggtga
      781 gacgggcctg ctacgcggcc gaccgcaccg gccacatgat cctgcagacg ctctaccagc
      841 agtgcgtgaa gcggggggtg cggttcttca acgagttcta cgtgctcgac ctgttgatga
      901 acgagggccg cacggccggc gtggtggcct atgagctggc caccggagag ctccacgtgt
      961 tccgggccac atcggtgctg ttcgccaccg gcggttcggg ccgcatgttc aaggtctcct
     1021 cgaacgccca cacgctgacc ggggacggac aggccgtcgt gtaccgccgc ggcgttcccc
     1081 tggaggacat ggagttcttc cagttccacc ccaccggcgt ctacaagatg ggcatcctcc
     1141 tgtctgaggc ggtccggggg gagggcggca tcctgctgaa cagcgagggg gagcggttca
     1201 tggagcgcta cgcccccacc gtcaaggacc tggcgccgcg cgacctggtc tcccgggcca
     1261 tcttccagga ggtccaggaa ggccggggca tcggtccggg caaggatggc gtgtacctgg
     1321 acgttcggca cctcccccgg gacgtgatcg aggagaagct ccccgacgtc accgagttcg
     1381 cccgcatcta cctgggcgtc gagcccacca cggagccggt cccgatccag cccaccgccc
     1441 actacacgat gggcgggatc cccacggacg tggacggcgt ggtggtgacc ggcgaggacg
     1501 agacccccgt gccgggcctg tacgcggcgg gcgagtgcgc ctgcgtcagc gtccacggcg
     1561 cgaaccggct ggggacgaac tccctgctgg acatcgtggt cttcggccgg cggggcggcc
     1621 ggcagatggc cgagttcgcg cgcgaggccg atccggtgga gccccccgag gaggccgagg
     1681 ccttcgtccg ggggatgctc ggtgagctgc tggcccgcag cagcggagag aacacggccg
     1741 acatccgctc ggagctgcag gacagcatgt acgacctggc gttcgtggtt cgcacggagc
     1801 agtccctgcg caagatggcc gagatcctga agggcctgca ggagcgcttc gaccgggccc
     1861 ccgtccagga caagggctcc cgctacaaca cggacctgat ggagacggtg gagctcgggt
     1921 tcctgctgga caacgccgag gcgctggtgg cctcggccct ggctcgcgaa gagagccgcg
     1981 gcgcgcacta ccgcgaggac cacccgatcc gcgacgacga tcactggctg aagcacacgc
     2041 tggccacccg ggaggcggac gggtccgtcc gcctcgacta caagccggtc cagctgggcc
     2101 cctacgtccc gatggagagg aagtactgat ggcggcgggc gagcagctcg gcgacgtgct
     2161 ggacgcctcc gaggagcggc gagccggggc cagcggcaag gtctcgctca cgctcaagat
     2221 ccgtcggtac aacccggagc tccggggcga ggagtcgtgg tgggacgagt tcaccatcga
     2281 ggcccacccg aacgaccgtc tcctggatgc cctgcacgag gtgaagtggc atcacgacgg
     2341 gaccctcgcc gtccggcggt cgtgcgcgca cggcatctgt ggctccgacg ccatgatggt
     2401 gaacgggaag aaccgtctgg cctgcaaggt gctggtccgt gaggtcgtgc ctcacgtgac
     2461 cgtcgaaccg atccgcggcc tgccggtgct gaaggacctc tatgtggaca tggagccctt
     2521 cttcgccggc taccgatcga tcatccccta cctggtgaac gacgagggcg agcccgaacg
     2581 cgagcgcctg cagtcgcccg aggagcggca ccggttcgac gacaccacga agtgcatcct
     2641 ctgcgggatc tgcaccacgt cctgcccgat cttctggggc gacgaggcct acgtgggacc
     2701 ggccgccatc gtgaacgccc accgcttcat ctacgacagc cgggaccgag ccgggagcga
     2761 gcgcctgaag atcctctcgg agaagaccgg cgtcttccgg tgccgcacca ccttcaactg
     2821 caccgaggcc tgcccccgcg ggatcgaggt gaccaaggcg atccaggacg tgaagcgctc
     2881 gatcctgttc gaccgcttct gagccctgcc ccacccgcct cccgggtgcg ggacgccggg
     2941 aacaccccgg ccggccacgt cccgaggatc cttcgatatc gtgcggccat ggcccatctg
     3001 gtgaccctga tccccggcga tggcatcggt cccgaggtcg cccaggccgc ccgccgggtg
     3061 ctcgatgcca ccggggtggg gttcgagtgg gtggtcgagc aggccggggc cgccgtgctg
     3121 gagcgggagg gcaccacgct ccccgaccgt gtgctcgagc agatccgacg cagccgggtg
     3181 gcgatcaagg gaccgatcac cacgccggtg ggccgcggct tccgcagcgt gaacgtggcc
     3241 ttgcggcacg acctggatct gttcgcggcc gtgcgaccgt cccgctccct gcccggggtc
     3301 cccacccggt acccggacgt ggacctggtg gtgatccgcg agaacacgga ggacctgtac
     3361 gcgggcatcg agttcgaggc aggagctccc gagacggatc ggctccgggc ccggctgcac
     3421 cagctggccg gggcggacct gcggcaggac gcgggcatca cgctcaagcc gatctcggtc
     3481 agcggggctc gccggatcgt gcggttcgcg ttcgagcacg cgcgggccaa cgggcgccgg
     3541 agggtgaccc tcgggcacaa ggcgaacatc atgctgttct ccgacgggct gtttttggac
     3601 atcgcgcgcc gggaggcgcc cgcgtacgcg gacgtggggt tcgaggagat gcagatcgac
     3661 cagctctcga tgcgcctgac ccgctcgccg gccgacttcg atgtcctgct gctacccaac
     3721 ctctacggcg acatcctctc ggacctctgc gccgggctcg tcggaggtct gggcctggcg
     3781 gccggcgcga acctcggctg ggagtacgcg gtcttcgagc ccgtgcacgg gagcgccccc
     3841 gacatcgccg gccgaggtct ggccaacccg gtcgcgatga tcctgtccgg ggtgatgatg
     3901 ctccgtcacc tgggcgagac ggccgccgcc gacgcggtgc gctccgcggt cgatcgggtc
     3961 ctgctggagg gggaggcccg gacacgggac ctgggcggca gcgcatccac ggaggaggtg
     4021 gcgacggcag tgacgaaggc cctgagcgcc ccgggtcggt cggggtggtc agacgggccc
     4081 cagcgcgcta gaaatcaagg gtagacgtcc acgggaggtc cggtatgacc gcgaaggtcg
     4141 gagacaggat cgtcgtcgag tccgagaccg tcggccaacc gatccgtgag ggcgagatcc
     4201 tcgaggtcct cgagggcgag gtggggatcc gctaccgcgt gcggtgggag gacggacacg
     4261 agacgatctt cacgcccagt ggagggagcg cgaggatcgt ctcggccgag agcgcccggc
     4321 gttagccgag agctcggccg atctcagtgc gagtggtgct gcccgggatc ccgaccggga
     4381 tcctccgatg ggagctgtca acagccgggt tcgccatcgt gcccgcagca gcccttgagc
     4441 agcaccacgg cacggaaccg gttccgcgcc gtgagccaga gcttggtccc gagcggtccc
     4501 tggtagctgc gccagttcgt gaagaaggcg tgtggggtgc gacgggcgtt ggccatcgtt
     4561 cctccatcct acggtcgccg gccggtcggt cggcgcggtc tagacgttcg tggggagccg
     4621 aaggctctcc ggaaggagtt cgttccggcg ccacgggcgg atggtctgag agtagaagtc
     4681 gcgaccttcg gcgtcgttga ccacccacac cgggaagccc tcgacccgca tctcgcggat
     4741 cgcctcgggg ccgagctccg ggaacgcgat gacccgctgg gacgagatcc gcagcgccgc
     4801 cagcgctcct cccccgccca gtgcggccag gtagaccgcg gtgtgccgcc gcagggcctc
     4861 gcggacgatc gggccgcggc cgcccttgcc gatgatgcct ttgaggccga tctccaacag
     4921 ccgaggggtg tacccgtcca tccggctggc ggtggtcggc ccggccgacc cgatcgcctt
     4981 gcccggccgc tccggcgtgg ggccgacgta atacacgatc tgccccgacg ggtcgaacgg
     5041 cagctcctgc ccggcatcga gcatctccac caggcgcttg tgcgcggcgt cgcgagccgc
     5101 gtagatcgtg ccggagacca gcaccacgtc tcccgccgtc agacggcgaa cgtcctggtc
     5161 ggacaggggg gccaggatcc gcttgcgccc gtcgtccagc agctcgaggc tcatggtcag
     5221 atcaccgtgg tcccggtccg gtgtgcgtgg cactcgaggt tcacggccac cggcagcgag
     5281 gcgatgtgcg tgggcatggt ctccacgtgg accgccaaca cggtctgggt cccgcccagc
     5341 gactgcggtc cgatcccgag cgcgttgcac ccttccagca ggtccgcctc gagctcggcc
     5401 acccggggat ccggactcgg caggcccacc ttgcggagca gggctcgctt ggccaggatg
     5461 gccgcgtact cgaagttgcc cccgatgccc acgcccacga tgcccggcgg gcaggcgtcc
     5521 gggccggcct cggccaccgt ggtgagcacg aaggtcgtca ccccctccag cccgtccgag
     5581 ggcttcagca tggcgtgacg gcccttgttc tccgagccgc cgcccttggc atcgacctgg
     5641 atccggacct ggtcgccggg gacgatgcgc gtgtggatga acgccggcgt gttgtcgccg
     5701 gtgttcgtcc gctcgaacac ggggtcacgg acgatcgacg tgcgcaggta cccctcgcgg
     5761 tagccccggc gcaccccctc gttgatcgca tcctccagat cggctcccac cacgtggacg
     5821 tcctgtccca cgtcgatgaa gaccaccgtg gagccggtgt cctggcagta ggggtactcg
     5881 ccctccgcgg ccagccgctg gttctccgcg atcaggtcca ggacccgacg accgagcggg
     5941 gactcctccc gccgccgggc gctcgccacc gcctccacca cgtcctcggg gatccggtag
     6001 ttgacccgga cgcacaggtc ccggaccgcg tcggtgatct gggcggcgtc gatctcccgg
     6061 accggtgcgc tggctgccat cccggccatc ctggcggaag gtgcttcgct ccgcccgggc
     6121 cagatggcac accgccgctc ggccatgggt cccgaactcc tctcgacggc gcgccccacc
     6181 cgcctgcggc tggcgggctt cctggccttg gagtggtggg ggcgcctggt ggcccggggg
     6241 tcgctgcgga cctgggccac ggtccgtccc ttccggaccg gcaccgcggg gatcgatctg
     6301 tgggaggggg agctgaccct ggccctcggc ctgagcgtcc tggtgggcgt gatcgtgatg
     6361 cgcatgctgg ccgcgcccgg cccggacgcg accggagccc gatccgaacc gggcccctag
     6421 ggaccgctca gagcttcgac aggtcctcgc acttggagcg gatgccctcg gcggccgcgc
     6481 ggagcgcacc gagctcgtcc tgggacaggt ccagctccac gacctcctcc accccgctgc
     6541 tggacagccg caccggcacc cccacgtaca ccccgtcgat cccgtactgg cccgtgcacc
     6601 acgcgcagac gggcagcatc tcctccgagt cgtgcacgat ggacgccacc atcttcgtga
     6661 tggaggccga gggcgcgtag taggcgctgc ccttcttcaa cagacccacg atctcggcgc
     6721 cagcgtctcg ggtgcgttgg tacagacgct ccagcgtgga ctcgtccacc agctccgtga
     6781 gcggcttgcc ccccacggtg gcatgcctgg gcagcgggac catcgcgtcc ccgtgcgacc
     6841 cgagcgtcat ggcctcgacg tccgagggcg agacccccag ctcctcagcg atgaagtagc
     6901 gcagcctgga ggagtccagg acgcccgcca tcccgatgac ccgctgcttg gggaagcctg
     6961 agacctcgga ggccaggaag gtcatctcgt ccagcgggtt ggtgaccatc acgatgatgg
     7021 cgtccggcga gccgtccctc acgttctcca cgacctgacg gatcacgccg gcgttcttgc
     7081 ccagcagatc catccggctc atgcccggct gccgggggaa gcccgccgtg atcaccacca
     7141 cgtcggatcc ggccgtctcc gcgtagtcgt tcgttccggt gatgaccggc tcgtacccct
     7201 cgacgggagc cgcctcgctc atgtccaggg ccagcccctg gggcaggccc tcgatgatgt
     7261 cgatcagcac gacctcggcc aggcccttct ccacgatccg catcgcggtg gtctgcccca
     7321 cgaacccgct gccgaccacg gtgaccttca tcgatcgctc cttcgatccg gggttcccat
     7381 cctatcgacg gcccgtccgt ggccccgctc gaccgcccgg gaggtcctca gccctgcagc
     7441 cgctcgatca cggcgtcggc cacctgcgag gtccccacgg cggtggggtc gtcgcgggtg
     7501 ggcttcatgt cgtaggtgac ggacctgccc tcctcgatca cggccgcgat ggccgcctcc
     7561 aggcggtcgg ccgcccccgg ctcgcccagg tgccggagca tcaggacgcc cgagagcatc
     7621 atggccatcg ggttcacctt gttcataccc ttgtacttcg gagcgctgcc gtgggtggcc
     7681 tcgaacaccg cggcctcgtc cccgatgttc gcgcccggcg ccagccccag accgcccacc
     7741 agacccgcgc acagatccga gacgatgtcc ccgtacaggt tgggcaggac cagcacgtcg
     7801 tactcttcgg gccgctgcac gagctgcatg gtgaggttgt ccacgatgcg gtcctcgaac
     7861 tcgatgtccg ggtacccggc ggcgacctgc cgggccgtgt ccaggaacag gccgtcggag
     7921 aacttcatga tgttggcctt gtgcacggcg gtcaccttgt ggcggccgta gcgccgagcg
     7981 tgctcgaagg cgaaccggac gatccgctcc gtcccccaca ccgagatcgg cttgatcgag
     8041 atgccggcgt cgtcgcggat ccgcttgccg gtggcaccgg ccacgaactc gatcagctcc
     8101 ttggcctcgg gcgtgccctg ctcgaactcg atccccgcgt acaggtcctc gtggttctcg
     8161 cggacgatca cgatgtccac gtcctggtac cgggagcgca cgccacggta ggacttgcag
     8221 gggcgcaggc acgagtacag gtcgagctcc ttgcggaggg ccacgttcac gcttcggaac
     8281 ccgctgccca ccggtgtggt gatggggccc ttgatggcga ccttggtgtc ccggatcgac
     8341 gccaacaccg gctccggcag cggcgtcccg tagcgctcca tcacgtcggc acccgcatcc
     8401 tggaggtccc agtcgaacgc gacgccggtg ccctcgagca cccggcgggt ggcctcggac
     8461 agctccggtc ccaccccgtc gcccgggatg aacgtcactc gatacgccat gtgctcacgc
     8521 tccttcgtgc gaccgttccc attgtgtcat ccgccggtcg ccctcacggc gccccttccc
     8581 ccggaaggcg gtacgctggt actgtctgac cgaaccatct cgacctcaag atatctcctc
     8641 gaagggagcc gtggatggcc gacaagggtc tcgaggaagt catcgccgcc tccacccggc
     8701 tctccgacgt ggacggcaag gccggacggc tgtggtacgt gggcttcggg atcgaggacc
     8761 tggcggagcg ctccacgttc gaggaggtgg tgtacctcct ccacaacctg cggctgccca
     8821 ccgagcccga gctcgacgac ctccacgagc gcctggtctc cgaacgcgag gtctcgacgt
     8881 tcctggcgga cctgatgccc accctcgccc agcagacctc ccccatgtcc atgctccgca
     8941 ccagcgtctc agcggcctcc gcctacgacc cggacgggtg ggagcagacc ccggccgcca
     9001 acgagcgcaa ggccctgcgg ctgatcgccc ggatccccac gatgatcgcg atgtaccatc
     9061 ggctgcgcac cgaccaggag gtcgtgctcc ccaaccccaa gctcccccac gcggccaact
     9121 tcctgtggat gctgctgggc gaggagccgg accaggagga cgcggcggtc ctcgacacca
     9181 cgctgatcct gtacgccgac cacacgatga acgcctccac gttcaccgcg cgcgtgatcg
     9241 cctccaccct cgcggacatg cactcggcca tcaccggcgc catggcggcc ctgaagggtc
     9301 cgctgcacgg cggcgccaac gaggaggcga tgaagctgct ggaggaggtg ggcgaccccg
     9361 cgaacgcgga gcgggtggtc aaggagaagc tggcccgcaa ggagcggatc atggggttcg
     9421 gtcaccgcgt ctacaagagc ggggaccctc gagcggcggt cctgcgccgc ctggccaagc
     9481 agaccggcga gcgcaagcga gacccgcggt ggtacgagat ctccgaggag atcgagcgcg
     9541 tggtgggcga ggagaagggg ctccacgcca acgtcgactt ctacgcggcc agcgtctacc
     9601 actacctggg cattccgcac gacctcatga cgccggtctt cgccctggca cgcacctccg
     9661 ggtggaccgc gcacgtgcgc gagcagtacg gggacaaccg cctgatccgg cccgagtccg
     9721 agtacatcgg accccgagac caggtctacg tgccgatcga ccagcggggc tgaaccgggc
     9781 cccctcagac cgggggaggc ggcgggggcg gcgggggtgg gggtgagatc gtccctcccc
     9841 cggcgcctcg atcccacacc c
//
LOCUS       DASQQQ010000276         5779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_47840, whole genome shotgun sequence.
ACCESSION   DASQQQ010000276 DASQQQ010000000
VERSION     DASQQQ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47840"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1629)
                     /locus_tag="VE646_10420"
     CDS             complement(<1..1629)
                     /locus_tag="VE646_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HZD80436.1"
                     /translation="MRIEDYAIIGDTHTAALVGLDGSVDWLCLPRFDSGALFASLLGD
                     EENGRWKIAPVGDVTRVERRYRRDTLVLETTFHTSEGVVRLVDCMPLRDRELNLVRLV
                     EGVSGRVPMRMDLTMRFDYGSIVPWVRRMDHGLLEAVAGPDAVDLRTPVPTEGRDFHT
                     VADFSVEPGDRIPFVLTWFRSHEPVPEPVDPLMAIDATVDFWQAWASRCRYEGEYRDA
                     VIRSLITLKALTYAPTGGIMAAPTTSLPERLGGSRNWDYRFCWLRDATFTLYSLLTAG
                     YEQEAAAWRDWLLRAVAGKPSELQIVYGPAGERRLTEFDVPWLSGYERSGPVRIGNAA
                     HQQFQLDVYGEVLDLLHQSSQAGMDHSGWTWELELKLLEFLESDWDRPDMGIWEVRGP
                     RRHFTHSKVMAWVAMDRGIRDAERFAPDSPEIHRWRALRDRIHQEVCDQGFDAEKNAF
                     VQFYGSKEIDASLLMVPLVGFLPPDDPRVRGTVEAVERELMLDGFVRRYAPDPQGSVD
                     GMNEDEGAFLPCSFWLADNYAMSGRTDEAHRLFRQ"
     gene            1884..2114
                     /locus_tag="VE646_10425"
     CDS             1884..2114
                     /locus_tag="VE646_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HZD80437.1"
                     /translation="MNAITTTVDARGQSCPGPLVSLARALKEARAGDLLELLATDPGS
                     RSDVPSWARISGNELLEATQEGDVFRYVVRKA"
     gene            2111..2680
                     /locus_tag="VE646_10430"
     CDS             2111..2680
                     /locus_tag="VE646_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HZD80438.1"
                     /translation="MSGSDRPQPEATALRELIDQALAEREREDAAGRNKITMVVWDGH
                     LDRVWPALILGTTAAASGMEVSIFFTFWGLFPLVKENKRLTGKEWMTKALSLMNPGSL
                     KRAKLSRYQFGGAGPWMLSKVAERYKTPHPTELLQLAQDMGVRLLPCQMTMDLMGVNR
                     EDLIDGLEEPIGAATALLEMKGSAIQLFI"
     gene            complement(2754..3113)
                     /locus_tag="VE646_10435"
     CDS             complement(2754..3113)
                     /locus_tag="VE646_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80439.1"
                     /translation="MEIGLLIRYGKIVPGRETEAVELFAEGEAFFTERLSKGEITFFE
                     PFFLSTSDLEEDLGFHLIKGDAPAIFKLMEDETYRALMTKMTLVMEHPRVDLLTVGEG
                     IPLQMERFAKVRVEIGM"
     gene            complement(3302..3901)
                     /locus_tag="VE646_10440"
     CDS             complement(3302..3901)
                     /locus_tag="VE646_10440"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HZD80440.1"
                     /translation="MAARFRGAISRLPPELAAEMRGTTWHPGCPVPLSSLRLLSLRYW
                     GFDGRVHEGPLVVDAAVAQPIVGVFRRLFRARFPIRRLHLAARYRPGHEDPNDRRDWT
                     AAFNCRPVVTPAGAGPIISQHAFGLAIDINPIQNPYVAADGFVRNHIARAYRDRSREL
                     PGMIHDGDVVVRAFAAIGWGWGGHWSSGKDYMHFSASGT"
     gene            complement(4081..4437)
                     /locus_tag="VE646_10445"
     CDS             complement(4081..4437)
                     /locus_tag="VE646_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80441.1"
                     /translation="MDLSVEPHALVLDLAYFLTGDAASQAAQDHGDEYPPPNDYYILN
                     DNPKLRTLVLAPHVRLRMLDWKSCCQTYFRGDLAAFADGFAHPDPAGTYRGPTSPYWL
                     WIREGQVVKIQEQYLP"
     gene            4484..4849
                     /locus_tag="VE646_10450"
     CDS             4484..4849
                     /locus_tag="VE646_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80442.1"
                     /translation="MGLGVDAVADGMDGDASGMDATADELGEIADGAGVDPVAEGPDA
                     EGPPPRAQAETRSTSKSGGNENGGRRRIGSLLVGPIVGSLPRAVGRLVHPADPLVLGG
                     MIPPARSPARWLPAGAYSS"
     gene            4882..5325
                     /locus_tag="VE646_10455"
     CDS             4882..5325
                     /locus_tag="VE646_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZD80443.1"
                     /translation="MAHTATAPDLTELASRLRLSVTRLARKLRQEAQYGISLTLLSTL
                     SAIDRAGRITIGELCAAEQVQPPTTSRAVAGLEEAGLVSRQHDQHDRRVSWLHVTPEG
                     RKLLQRSRRRKEAFLAKRLRELEPAEIEVLEHAAGILEELVEGAQ"
     gene            5322..>5779
                     /locus_tag="VE646_10460"
     CDS             5322..>5779
                     /locus_tag="VE646_10460"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD80444.1"
                     /translation="MTRVGLAVHRTFHAVSHSRNFRLFFAGQLISVTGTWMQSVASAW
                     LVLKLTGSGVALGFQTALNFGPVLFLAAWGGLLADRLDKRKILLGTQSSFAVLALALW
                     ALVATGTVQLWHVYVLSALQGVVTSFDNPTRQSFYAEMVGQRDLSNAVSL"
BASE COUNT          910 a         1967 c         2044 g          858 t
ORIGIN      
        1 ctgccggaag agccgatggg cctcgtccgt gcgccccgac atcgcgtagt tgtcggccag
       61 ccagaagctg caggggagga aggcgccttc gtcctcgttc atgccgtcca ccgacccctg
      121 aggatcgggc gcgtagcgac ggacgaagcc gtcgagcatc agttcgcgct cgaccgcctc
      181 caccgtgcct cgcacccgtg gatcgtcggg gggcaggaac ccgaccaggg ggaccatcag
      241 caaggatgcg tcgatctcct tcgagccgta gaactggacg aacgcgttct tctcggcatc
      301 gaacccctgg tcgcacacct cctggtggat ccggtcgcgc agcgctcgcc atcggtggat
      361 ctccggtgag tccggagcga agcgctcggc gtcgcggatc ccgcggtcca tggccaccca
      421 ggccatgacc ttggagtgcg tgaagtggcg ccgaggaccg cggacctccc agatgcccat
      481 gtcgggccga tcccagtccg actcgaggaa ctccaggagc ttcagctcca gctcccaggt
      541 ccagccggag tggtccatcc cggcctggct ggattgatgc agcagatcga ggacctcccc
      601 gtagacgtcg agctggaact gctggtgcgc ggcattaccg atccggacgg gccccgaccg
      661 ttcgtaaccg gacaaccagg ggacatcgaa ctccgtgagg cgacgctcgc ccgcaggtcc
      721 gtacacgatc tggagctccg agggcttgcc ggccaccgcc cgcagcagcc aatcgcgcca
      781 ggcggccgcc tcctgctcgt agcccgcggt gagcagcgag tacagggtga aggtggcgtc
      841 ccgcagccag cagaaccggt agtcccagtt gcgcgatccg ccgagccgct cgggcaggga
      901 ggtggtgggc gcggccatga tgccgccggt gggggcgtac gtgagggcct tcagcgtgat
      961 cagggaacgg atcaccgcat cgcggtactc gccctcgtag cggcagcgcg aggcccacgc
     1021 ctgccagaag tcgaccgtcg cgtcgatcgc catcagggga tccaccggct cggggaccgg
     1081 ctcgtgcgag cggaaccagg tgaggacgaa cgggatccga tcccccggct ctacgctgaa
     1141 gtcggccacc gtatggaagt cccggccctc ggtcggcacc ggggtgcgca ggtcgaccgc
     1201 gtcggggccg gccacggcct ccagcaggcc atggtccatg cggcgcaccc aggggacgat
     1261 ggatccgtag tcgaagcgca tcgtgagatc catgcgcatc gggacccggc cgctcacccc
     1321 ctccaccagg cggaccaggt tcagctcccg gtcgcggagc ggcatgcagt cgaccagccg
     1381 caccacgccc tcggacgtgt ggaacgtggt ctcgagcacc agggtgtccc gtcggtaccg
     1441 ccgctccacg cgggtgacgt caccgacggg cgcgatcttc cagcggccgt tctcctcatc
     1501 acccaggagg gaagcgaaca gagcaccgga gtcgaaccgg gggaggcaga gccagtccac
     1561 cgatccgtcc agacccacca gggcggcggt gtgggtgtcc ccgatgatcg cgtagtcctc
     1621 gatgcgcacc gtccggaatc ctatctgggc tccgcggttc cgcccgggtc ggcggttcgg
     1681 gccggcggcc ctaccttgcc ccatatccca ccgctgcgat aggatttgca cctgcgatga
     1741 tcaggacacg gtgccgcggg ctcgttgggg cgatgatccc ctgccggtgc tgctgtccca
     1801 tggtgcccga agcgggccgc tagatcccgc tacgggcacc gcgaccggcc accatccgtc
     1861 cgtccctcac cggaggtcat cccgtgaacg ccatcaccac caccgtcgat gcccggggcc
     1921 agtcgtgccc gggcccgctc gtctccttgg cccgggccct caaggaggct cgcgccggcg
     1981 atctgctgga gctgctggcc acggacccgg gatccaggtc cgacgtcccc tcgtgggcgc
     2041 ggatctcggg caatgagttg ctcgaggcca cgcaggaggg cgacgtgttc cgctacgtgg
     2101 tgaggaaggc atgagcggaa gcgatcggcc ccagccggag gcgaccgccc tccgcgagct
     2161 catcgatcag gcgctggccg aacgcgagcg agaggacgcc gcgggacgca acaagatcac
     2221 catggtggtc tgggacgggc atctggaccg ggtctggccg gccctgatcc tcggcaccac
     2281 ggcggccgcc agcggtatgg aggtatcgat cttcttcacg ttctgggggc tgttccccct
     2341 ggtgaaggag aacaagcggc tcaccgggaa ggagtggatg accaaggcgc tgtccctgat
     2401 gaacccgggg agcctgaagc gcgcgaagct cagccgctac cagttcgggg gggcggggcc
     2461 atggatgctg tcgaaggtgg ccgagcgtta caagacgccg caccccacgg agctgctgca
     2521 gctggcacag gacatggggg ttcggttgct gccctgccag atgacgatgg atctgatggg
     2581 cgtgaaccgg gaggacctga tcgacgggct cgaggagccg atcggggcag ccaccgcgct
     2641 gctcgagatg aagggctccg cgatccagct gttcatctga tcgggaacca ccggcatggc
     2701 gggggggagc cgccgggacc gcaagcggct cccccccaaa agagcccccg ggctcacatc
     2761 ccgatctcga cgcggacctt ggcgaagcgc tccatctgga gcgggatgcc ctcgcccacg
     2821 gtcagcaggt cgacccgcgg gtgctccatc accagggtca tcttggtcat gagcgcccgg
     2881 taggtctcat cctccatgag cttgaagatc gccggagcat cgcccttgat caggtggaag
     2941 cccaggtcct cttcgaggtc gctggtcgac aagaagaacg gctcgaagaa ggtgatctct
     3001 cccttcgaga gccgctccgt gaagaacgcc tcaccctccg cgaacagctc gaccgcctcg
     3061 gtctcccggc cgggcacgat cttgccgtag cggatcaaca gaccgatctc catgtcgttc
     3121 cctccccgaa cgtcagcagc ggtcgacctc cggtcgagat ggctcggtca cctccctcat
     3181 cccaccggga accggaccgc gtaccaccca tccgaaccct cggtgagccg ccggtccagg
     3241 ggcgctggtc ccacggctct cgggaccgcc ctgcgatggc ccccgacctg tcctgagcgg
     3301 atcaggtccc agaggcggag aagtgcatgt aatccttgcc cgaggaccaa tgcccgcccc
     3361 acccccatcc gatggcggcg aaggcgcgca cgaccacgtc cccgtcatgg atcatgccgg
     3421 gaagctcccg gctgcggtcc cgataggccc gagcgatgtg gttgcggacg aagccgtcgg
     3481 cggccacgta ggggttctgg atcgggttga tgtcgatggc cagcccgaag gcgtgctggg
     3541 agatgatggg cccggcgccg gctggcgtga ccactgggcg gcagttgaac gcggcggtcc
     3601 agtcgcgacg gtcgttcgga tcctcgtggc ccggtcggta ccgggcggcc aggtggagcc
     3661 gccggatcgg gaaccgggcc cggaacaacc ggcggaagac gcccacgatc ggctgggcca
     3721 ccgcggcgtc gaccacgagt gggccctcgt ggacccgccc atcgaacccc cagtaccgca
     3781 ggctcagcag ccggaggctg gacaggggca cgggacagcc cgggtgccag gtggtgccgc
     3841 gcatctcggc ggcgagctcg ggaggtagcc gcgagatcgc tcccctgaac cgggcggcca
     3901 tcgccggtgt gaccgatggc gtcggggagt cggtaccggg ggccgagggg cccgatggag
     3961 tccgttgggc ggatggggtt cgctcgacgg atggtcggcc acccggcgcg ccacccgtgg
     4021 cggaggtgca ggcggcgagc aggaccagca ccgtggccgc cccgagcagg cggctccgcg
     4081 tcacgggagg tactgctcct ggatcttcac gacctgccct tcccggatcc acagccagta
     4141 gggggaggtc gggccccggt aggtgcccgc ggggtcgggg tgggcaaagc cgtcggcgaa
     4201 cgcggccagg tctccccgga agtacgtctg gcagcagctc ttccagtcga gcatccgcag
     4261 tcgcacgtgg ggggcgagga ccagcgtccg gagcttcggg ttgtcgttca ggatgtagta
     4321 gtcgttcggt ggtgggtact cgtccccgtg atcctgcgcg gcctgcgacg ccgcgtcacc
     4381 ggtcaggaag tacgcgaggt ccagcacgag ggcgtggggc tcgaccgaca ggtccaccga
     4441 gtggacgaac ccgaagtgtt tcccatcctc caaagcggcg ggggtggggc tgggcgtgga
     4501 cgcggtggcc gacggtatgg acggcgacgc gtcgggtatg gatgcgacgg cggacgagct
     4561 cggggagatc gctgacgggg ccggggtgga cccggtcgcc gaggggcccg acgcggaggg
     4621 gccgccgccc cgggcgcagg cggagaccag gagcaccagc aagagcggcg gcaacgagaa
     4681 cggcgggcgg cgacgcatcg gttccctcct cgtagggccc atcgtcggca gtctcccacg
     4741 ggcggtgggc cgactcgtgc atccggccga cccattggtc ctgggaggga tgatcccccc
     4801 ggctcggtcg cccgcccggt ggctgccggc cggggcatat agttcgtgac gctaattagc
     4861 tttgcgaact atactgggcg tatggcccac accgcgaccg cacccgatct gaccgagctg
     4921 gcctcgcgcc tgcggctgag cgtcacccgc ctggcccgca agctgcgcca agaggcccag
     4981 tacggcatct cgctgacgct gctctcgacg ctatcggcca tcgatcgcgc cgggcgcatc
     5041 acgatcggcg aactgtgtgc cgccgagcag gtccagccac ccaccaccag ccgtgccgtg
     5101 gccggcctgg aggaggcggg cctggtctcg cgccagcacg atcagcacga tcgccgggtg
     5161 tcgtggctcc acgtgacccc cgagggacgc aagctcctcc agcgcagccg ccgacgcaag
     5221 gaagccttcc tggccaagcg gctccgggag ctggagccgg cggagatcga ggtcctcgag
     5281 cacgccgccg ggatcctgga ggagctggtg gagggagccc agtgacgcgg gtgggcctgg
     5341 cggtccaccg caccttccac gccgtctccc actcgcggaa cttccggttg ttcttcgctg
     5401 ggcagctgat ctcggtcacg gggacctgga tgcagtcggt ggcctccgcg tggctggtcc
     5461 tcaagctgac cggtagcggg gtggcgctgg gcttccagac cgcgctgaac ttcggtcccg
     5521 tcctgttcct ggccgcctgg ggcggtctgc tggccgaccg gttggacaag cgcaagatcc
     5581 tcctggggac ccagagctcg ttcgcggtgc tcgcgctcgc cctgtgggcg ttggtggcca
     5641 ccgggaccgt gcagctctgg cacgtctacg tgctctccgc cctccagggg gtggtgacct
     5701 ccttcgacaa ccccacccgg cagagcttct acgcggagat ggtggggcag cgcgacctgt
     5761 cgaacgcggt gagcctgaa
//
LOCUS       DASQQQ010000277         6892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_17778, whole genome shotgun sequence.
ACCESSION   DASQQQ010000277 DASQQQ010000000
VERSION     DASQQQ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17778"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VE646_10465"
     CDS             complement(<1..530)
                     /locus_tag="VE646_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80445.1"
                     /translation="MNGSGRPAAFRLALRLLWKQALAWTGVLGVGAVAITQGYQNAYP
                     SLQSRMIIAAQIGANPAFQALNGKAVNLDTLGGFVAWRYGGPVVVIVAIWGLLAATKL
                     LRGEEETGHGELVLGGRLRPTMLLAADLAALGLVFAVAWAVSGAALVGGGVQAAGAFL
                     VAMQVVLGAVTFAAIGA"
     gene            complement(527..1417)
                     /locus_tag="VE646_10470"
     CDS             complement(527..1417)
                     /locus_tag="VE646_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017837576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD80446.1"
                     /translation="MSVDAVIRTEGLTKRFGATVALDDLTLGVERGEVLGYLGPNGAG
                     KTTTTRLLLGLLRPTAGTASILGLDPWADTVELHARLAYVPGELAVWPQLTGGQMLDL
                     LGALHGSVDPAYRDELCQRFEFDPSKKGRSYSKGNRQKVGLIAALMTRAEILILDEPT
                     AGLDPLMEMTFRGCVGEAREHGQTIFLSSHILSEVEAVCDRVAILRQGRLADLGTLES
                     LRAMSAREVEVRFDGSVPDLTQIPGVTAVRVEDGTARVQVRGSADPLIKALATHEVAQ
                     ITAREPSLEEVFLTYYGDEG"
     gene            1507..3597
                     /locus_tag="VE646_10475"
     CDS             1507..3597
                     /locus_tag="VE646_10475"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HZD80447.1"
                     /translation="MFDALGRLNPAQRDAVEHPGGPLLVVAGAGSGKTWTLACRVARL
                     VERGVRPERILLLTFTRRAAREMLSRAERLTGDGTLGRVWGGTFHGLGNRLLRIHGRP
                     IGVPADFTVLDRTDTADLMDLIRGELGLGQGKRRFPRKETLADIYSRTVNAQERLSAV
                     LERDFPWCADQVEGMRSIFERYTERKRAQRCLDYDDLLVYWRALSLAPGAGARIAGMF
                     EHVLVDEYQDTNALQADILEALKPDGEGLMVVGDDAQSIYSFRAATVRNILEFPARFP
                     GTTIVRLEQNYRSTQPILDATNAVIALSPQRHEKTLWSERATGARPRLLTCLDEPDQS
                     EAVCRAVLENRERGVLLKDQAVLFRAAHHSDLLEVELSRRNVPFVKYGGLAFMESAHV
                     KDALAVLRLLDNPRDEVSWFRVLQLLDGVGPATARRLMVVLGVVGEDPSGRSPLAALL
                     QLDLAVPAPARPELEDLRGVVRDCADDVPPSVQLERVRRFLEPILARRYDRPDARLRD
                     LEQLEQLAVGYPTRERFVTDLTLDPPSATGDLAGPPLLDEDYLVLSTIHSAKGGEWDV
                     VHVIHAADGMIPSDMATGDRDQIEEERRLFYVALTRARDELRVYAPLRYHRRNRGRED
                     RHLHAQLTRFLPPAVLDRFERRTTSSDPDRAPGGAVTVSSSGPGSTSAGMDGTPAEVD
                     RFLTGLWALQSGPD"
     gene            complement(3653..3892)
                     /locus_tag="VE646_10480"
     CDS             complement(3653..3892)
                     /locus_tag="VE646_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80448.1"
                     /translation="MTKLGVQVDDPQIASIPVAVPPLVLQASVAIGAVRRAARRGMRE
                     DTPITIRCSAPVRRGGKPPTEGGVRRRAGRSRPDR"
     gene            complement(4225..4367)
                     /locus_tag="VE646_10485"
                     /pseudo
     CDS             complement(4225..4367)
                     /locus_tag="VE646_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004967407.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            4620..5486
                     /locus_tag="VE646_10490"
     CDS             4620..5486
                     /locus_tag="VE646_10490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZD80449.1"
                     /translation="MRVVRTDDVTAFLDLAGPLLRRDEARNNLVLGLVASIVADPRAH
                     EEHDAWIALDPGPVAAAIRTPPRQLLVADAAEPSAIEPLVHAIAEDAPTLPGATGNRP
                     TIDDFGQAWTRTTGRVARVGSDLTVFALRRVAEVHRAPGSMRAAVREDRDLLVAWLRE
                     FTAEAPLPDEIVANELETFLEGRLEARNAGYRLWEADSRPVSLAGFIGPRQDGARIGP
                     VYTPSEHRGAGYARSLLAELARELLDRGYHACYLASDDDNRAATALYTSVGFEPVAHA
                     AMIVFERSSPAS"
     gene            complement(5531..>6892)
                     /locus_tag="VE646_10495"
     CDS             complement(5531..>6892)
                     /locus_tag="VE646_10495"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZD80450.1"
                     /translation="RLGFTPIEPAVRVFAPQPDGSALTLWADPVRTAQELRSRSAHDA
                     AAYPGFDKRVRSVASFLTYLNVATPPDVKNLTFADLLTGLRAGKAFRDLGKKASRETT
                     RVLPMAVADLVAEDFESDAVRGAIASRAVLYAAMGPWSAGTAFLLLSDSAGNDGGAAG
                     QSTQSRGGPGALAQALADAARSFGAEIRTGAEVVRVLLEEHRAVGVVLSTGEEIRATA
                     VISNADPKRTLTTLVDPVVLGPHLVWRAKNIRTPGVVSKVNLAVSELPTFVGAEELEL
                     LRGRIVIAPSVDHLERAFDASKYGRISEEPFLELTIPSLSDPTLAPAGRHVVSVIVQW
                     TPYALRAGDWKAEGKRVGDLVVDTLERYAPGFSKLVTATQVLTPLDLEAEYGLTEGHP
                     LHAEPGLDQFFAWRPLLGHARYRFGVDGLYLCGSGAHPGGGVTGGPGANAAREILSDL
                     KKG"
BASE COUNT          970 a         2629 c         2357 g          936 t
ORIGIN      
        1 gccccgatcg ccgcgaacgt caccgctccc agcaccacct gcatcgccac caggaaggcg
       61 cccgccgcct gcaccccgcc cccgaccagc gccgcccccg acaccgccca ggccaccgcg
      121 aacaccagcc ccagggcggc cagatcggcc gccagcagca tggtgggccg gagccgccct
      181 cccaggacca gctccccgtg ccccgtctcc tcctcgcccc ggagcagctt cgtggccgcc
      241 agcagtcccc agatcgccac gatcacgacc accgggccgc cgtaccgcca ggccacgaag
      301 ccgccgaggg tgtccaggtt gacggccttg ccgttgaggg cctggaacgc cgggttcgcg
      361 ccgatctgcg ccgcgatgat catccgggac tgcaggctcg ggtaggcgtt ctggtatccc
      421 tgggtgatgg cgacggcgcc cacccccagg accccggtcc acgccagcgc ctgcttccag
      481 agcagccgca gggccagccg gaacgcggcc ggacggcccg agccgttcac ccctcgtccc
      541 cgtagtaggt caggaagacc tcctccagcg agggctctcg cgcggtgatc tgagcgacct
      601 cgtgcgtggc cagcgccttg atgagcggat cggcactccc ccggacctgg acccgcgccg
      661 tgccgtcctc caccctcacg gccgtcaccc cggggatctg ggtcaggtcg gggaccgagc
      721 catcgaagcg cacctcgacc tcgcgggccg acatcgcgcg cagcgactcc agcgtcccca
      781 ggtccgccag ccgcccctgc cgcaggatcg cgacccggtc gcacacggcc tcgacctccg
      841 agaggatgtg cgaggacagg aagatcgtct gcccgtgttc cctggcctcg cccacgcacc
      901 cccggaaggt catctccatc aacgggtcca ggccggcggt gggctcgtcg aggatgagga
      961 tctcggccct ggtcatcagc gccgcgatga gcccgacctt ctgccggttg cccttcgagt
     1021 acgagcggcc cttcttggac gggtcgaact cgaagcgctg gcacagctcg tcgcggtagg
     1081 ccgggtcgac gctgccgtgc agcgcgccga ggaggtcgag catctgcccg ccggtgagct
     1141 gcggccacac ggcgagctcg cccgggacgt acgccaggcg cgcatgcagc tccacggtat
     1201 cggcccaggg gtcaagaccg aggatgctgg cggtgccggc ggtcggtcgc aggagcccca
     1261 ggaggagccg cgtcgtggtg gtcttgccgg cgccgttcgg acccaggtag ccgagcacct
     1321 caccgcgctc gacccccagg gtgaggtcgt ccagagccac ggtggccccg aaacgcttcg
     1381 tcagcccctc ggtacggatc accgcgtcca cgctcacccc cgcagcaggc gaccgggggc
     1441 agcttacgag gcacgacggc gggctgcttg cccgggctcc gtcgctcccc tacgcttgac
     1501 gaacacatgt tcgatgcact cggtcgactg aaccccgccc agcgcgacgc cgtggagcac
     1561 ccgggaggac cgctgctggt ggtggcgggc gccggcagcg gcaagacctg gacgctggcc
     1621 tgccgggtgg ctcgcctggt ggagcgcggc gtgcggcccg agcggatcct gctgctcacc
     1681 ttcacccgcc gggccgcccg cgagatgctg agccgcgcag agcggctcac cggcgacggg
     1741 acgctcgggc gcgtgtgggg cgggacgttc cacggcctcg ggaaccggct gctgcggatc
     1801 cacggtcgcc cgatcggcgt ccccgcggac ttcaccgtgc tcgaccgcac cgacacggcc
     1861 gacctgatgg acctgatccg gggcgagctg ggcctgggcc aggggaagcg acggttcccg
     1921 cggaaggaaa cgctcgccga catctactcg cggaccgtca acgcgcagga gcggctgagc
     1981 gccgtgctcg agcgcgactt cccctggtgt gcggaccagg tcgaggggat gcgctcgatc
     2041 ttcgagcgct acacggagcg caagcgcgca caacggtgtc tggactacga cgacctcctg
     2101 gtctactggc gggcgctgtc cctggcgccg ggtgccggtg cgcggatcgc cgggatgttc
     2161 gagcacgtcc tggtcgacga gtaccaggac accaacgccc tgcaggccga catcctggaa
     2221 gccctcaagc ccgacgggga gggactgatg gtcgtgggcg acgatgccca atcgatctac
     2281 tcgttccgcg cggccacggt ccgcaacatc ctggagttcc cggcgcgatt ccccgggacc
     2341 acgatcgtcc ggctcgagca gaactaccgc tccacgcagc ccatcctgga cgccacgaac
     2401 gcggtgatcg cgctctcgcc ccagcggcac gagaagaccc tgtggtccga gcgcgcgacc
     2461 ggggcccgcc cacggctgct cacctgtctc gacgagcccg accagtccga ggccgtgtgc
     2521 cgggccgtgc tcgagaaccg tgagcgcggc gtgctcctca aggatcaggc cgtgctcttc
     2581 cgggccgcgc accacagcga cctgcttgag gtcgagctct cacgccggaa cgtccccttc
     2641 gtgaagtacg gcggcctggc gttcatggag tcggcgcacg tgaaggacgc cctcgcggtc
     2701 ctccggctcc tggacaatcc ccgcgacgag gtgagctggt tccgcgtcct gcagctgctc
     2761 gacggcgtgg ggccggccac ggcccggcgg ctgatggtgg tgctgggggt ggtaggcgag
     2821 gacccgagcg gccgctcacc gctggccgcc ctgctccagc tcgatctggc cgtccccgcg
     2881 ccggcccggc ccgagctcga ggacctccgg ggcgtggtgc gcgactgcgc cgacgacgtc
     2941 ccgccatccg tccagctcga gcgcgtccgc cggttcctcg agccgatcct ggcccgccgt
     3001 tacgatcgtc ccgacgcgcg cctgcgcgac ctcgagcagc tcgagcagct cgccgtcggc
     3061 taccccacgc gcgagcggtt cgtcaccgac ctcacgctcg acccgccatc ggccaccggc
     3121 gacctggccg ggccgccgct gctggacgag gactacctcg tgctctccac gatccactcg
     3181 gccaagggcg gcgagtggga cgtggtccac gtgatccacg ccgcggacgg catgatcccc
     3241 tcggacatgg ccaccgggga tcgggatcag atcgaggagg aacggcggtt gttctacgta
     3301 gcgctgacgc gggcccggga cgagctccgc gtgtacgcgc cgctccggta ccaccggcgc
     3361 aaccgggggc gcgaggatcg tcacctccac gcccagctca cgcggttcct cccgccggcc
     3421 gtgctcgacc ggttcgagcg gcggaccacc tcctccgatc cggaccgtgc ccccggcggg
     3481 gccgtcacag tctcgtccag cggtccgggc tcgacctcgg cagggatgga cggcacacct
     3541 gccgaggtcg accgcttcct gaccgggctc tgggctcttc agtcgggtcc agactagcgg
     3601 gctccggagg cgtggccgag agccgttccc gcacccggcc gccgagagcg cctcagcggt
     3661 ccggtcggga tcggccggcg cgtcgccgga cacctccttc cgtcggcggc tttcccccac
     3721 gccgaacggg cgccgagcag cggatcgtga tcggggtgtc ctcccgcatc ccccgccgcg
     3781 ccgcccggcg caccgcgccg atcgccacgc tcgcctgtag caccagcggc gggacggcca
     3841 cggggatgga ggcgatctgc ggatcgtcca cctggacccc gagcttggtc acccgccggg
     3901 ggctcgtcac cacgagctcg accaggacct ctcgccctga tagaccctgg gcggcgccgg
     3961 cgctcgaccc ggatgccgcg taccatccgg ccgggcagca ccaggccggc caccgtgact
     4021 ccggacagga tcgacgccag caccagcagc cacccggcct gcacgctggt gcccaccacg
     4081 aacagcagga gcacgcccgg gagcaaccga tcggtggccc aggacggccg gcgccagcga
     4141 cttcacgtca tcgggaagga ggaaggtccg tccttcgctc accgcccggg ccgggagcac
     4201 cggccggcgg tcctcgatgg catgccggtg gtacacggag atgccggtga ggtcggaggg
     4261 cagcaggtcc ggcgtcgcct ggacccggct gaaggtgccc tcgatcgatg ccgcgatggt
     4321 ccgcgccagc tcgtcttgcc ggtccccggg acgccctgga ggagcagttg ccgccggcga
     4381 acagacagat caccgcggtc ctccaggaac tgccgaccgg cgctcgcgcg ctccacgttg
     4441 tcgaccacgc gacgaagcgc tgccgaggtt cgtgccagat cggatgcggt ggtgggcatc
     4501 cccccgcttc gcatcgacgg gcccccatgc tgctcgagca agcgtgcccg tccgtcggcg
     4561 ccatcagggc atctcgtgca tctgctcatc ccaccgatca gccggtagcc tgtaccgcga
     4621 tgcgggtcgt ccggaccgac gacgtgacgg cgttcttgga cctggccggt ccgctccttc
     4681 ggcgcgacga ggcccgcaac aacctcgtcc tggggctcgt ggcctccatc gtggcggatc
     4741 cccgagccca cgaggaacac gacgcctgga tcgcgctgga ccccggcccg gtggcggctg
     4801 ccatccggac gccgccacgg cagctcctcg tagccgacgc ggccgagccc tccgcgatcg
     4861 agcctctggt ccacgcgatc gccgaggacg ctcccaccct gccgggtgcc accggcaacc
     4921 ggcccacgat cgacgacttc gggcaggcct ggacccggac caccggccgc gtcgcgcggg
     4981 tcggctccga cctcaccgtc ttcgccctcc gacgggtggc cgaggtccac cgggctcccg
     5041 gctccatgcg tgcggcggtg cgcgaggacc gggatctcct cgtggcctgg ctccgcgagt
     5101 tcaccgccga ggcacccctc ccggacgaga tcgtggccaa cgagctcgag acgttcctgg
     5161 agggacggct cgaggcccgc aacgcgggct accggctgtg ggaagccgac tcccggccgg
     5221 tctcgctggc cggcttcatc gggccccggc aggacggcgc ccgcatcgga ccggtctaca
     5281 cgccgtccga gcatcgcggc gccggctacg cgcggagcct cctggccgag ttggcccgag
     5341 agctgctcga tcgcgggtat cacgcctgct acctggcgtc cgacgacgac aaccgggccg
     5401 ccaccgccct ctacacatcg gtgggcttcg aaccggtcgc ccatgccgcg atgatcgtgt
     5461 tcgagcggtc atctccggcg agctgaccct cccgcggccc tcgccaccgc gcccaggtga
     5521 tgggccggga tcagcccttc ttcaggtcgc tcaggatctc ccgggcggcg ttcgctcccg
     5581 gtccgccggt cacaccgccg cccggatggg cgccggagcc gcacaggtac agtccgtcca
     5641 cgccgaaccg gtaccgcgcg tgccccagca ggggtcgcca ggcgaagaac tggtcgaggc
     5701 cgggctccgc gtgcaggggg tggccctcgg tgagcccgta ctcggcctcg aggtcgagcg
     5761 gcgtgagcac ctgggtggct gtcaccagct tggagaaccc tggcgcgtac cgctccagcg
     5821 tgtccacgac caggtcgccc acgcgcttgc cctctgcctt ccagtcgccg gcgcggagcg
     5881 cgtacggcgt ccactggacg atcacgctca ctacgtgcct gcccgcgggc gccagcgtgg
     5941 gatccgagag cgaggggatc gtgagctcca ggaacggctc ctcgctgatc cgtccgtact
     6001 tggaggcatc gaaggcccgc tccaggtgat cgacgctcgg agcgatcacg atgcgtccgc
     6061 gcagcagctc cagctcctcg gcccctacga aggtcggcag ctccgagacc gccaggttca
     6121 ccttcgagac cacgcccggc gttcggatgt tcttcgcccg ccagaccagg tgcggcccaa
     6181 gcaccaccgg gtccaccagg gtggtcaggg tgcgcttggg gtcggcgttc gagatcacgg
     6241 ccgtggctcg gatctcctcc ccggtggaga ggacaacgcc cacggcacga tgctcctcga
     6301 gcaggacccg gacgacctcg gcgccggtcc ggatctcggc gccgaaggat cgggccgcat
     6361 cggccagcgc ctgggccagc gccccgggcc cgccccggga ctgggtggac tgccccgcgg
     6421 ccccgccgtc gttgccggcc gagtccgaga gcagcaggaa ggccgtcccc gccgaccacg
     6481 ggcccatcgc cgcgtacagc accgcgcgcg aggcgatcgc gccgcgcacg gcgtcgctct
     6541 cgaagtcctc ggccaccagg tcggccaccg ccatcggcag cacccgcgtg gtctcacgcg
     6601 acgccttctt gcccaggtct cggaacgcct tcccggcgcg caggccggtg agcaggtcgg
     6661 cgaaggtcag gttcttcacg tcgggcgggg tggccacgtt caggtaggtc aggaagctcg
     6721 ccacggagcg cacccgcttg tcgaaccccg gataggctgc ggcgtcgtgc gccgaccggg
     6781 accgcagctc ctgcgccgtc cggaccggat ctgcccacag cgtgagggcc gagccgtcgg
     6841 gctggggtgc gaacacccgg accgccggct cgatcggcgt gaaccccagc cg
//
LOCUS       DASQQQ010000278         2923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_17841, whole genome shotgun sequence.
ACCESSION   DASQQQ010000278 DASQQQ010000000
VERSION     DASQQQ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17841"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VE646_10500"
     CDS             complement(<1..1175)
                     /locus_tag="VE646_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HZD80451.1"
                     /translation="MSEFDLGIHGATVVGPSRRARLHLYVHQGRVAAVSPERRDAATT
                     VDATGLYLLPGMVDTHVHLMDPGATEREDFPTGTAAAAVHGVTTIVEHTHGHPVRTVQ
                     ELERKRWHLRSRSHVDYGLAAHVWPDQIGRLGELWAAGVAFFKIFTCTTHGVPGLDAA
                     HLLAALEDLARAGAAALIHCEDDTITGEAERALRKAGRDDGAVLLQWRSREAELVATQ
                     VAALLSGITGAHATIAHVSHPAVADRIAAERAAGADVAAEACPQYFLLREDEVLEKGA
                     FRKFTPPARARTDADEDAMWDRLRSGVLTHVSTDHAPATRQQKAQGIWEAAFGLPGLD
                     TTLPLLIDAALRGKLALEDVVRAYAEGPARRYGLWPRKGTLAPGADADLVLVDPGPTS
                     "
     gene            complement(1172..2170)
                     /locus_tag="VE646_10505"
     CDS             complement(1172..2170)
                     /locus_tag="VE646_10505"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZD80452.1"
                     /translation="MTTVQARQLVRSTRWPKAAAILVLIGVAAWIPQRGSTFTASLAL
                     NILIFGLLATSLDLMAGYTGLVSLGHAAFLGIGAYGIAIATNHHISPNMAIPIALAAV
                     LLAAGILGVVAVRVRDLTFVILTLALSQLVWGLALLWVSVSGGDNGLSVNSLPVLGPF
                     DLNTPNTLYYFVLVIFLLCETLLWAIVRSPFGLSLQGIRDNEQRMRTLGYNVSLHKYL
                     AFVISSSLAGVAGILYAFYNLYVTPNSIDFSHNGIIVMMAIVGGLGTLWGPLVGAGVV
                     VFIQLYVSIHVTRWVTLLGVVFVLTVLFAREGLWGTFLKLPGMLARIRQRRSSPVA"
     gene            complement(2167..>2923)
                     /locus_tag="VE646_10510"
     CDS             complement(2167..>2923)
                     /locus_tag="VE646_10510"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZD80453.1"
                     /translation="LAHGAIYLVGGYVAYSTIVVTHNFGLGLLAAALAMAVLGVVLER
                     GLLARLRGLPLAELLLTLGIAFVMGDSSLSLWGGDPISIRTPSWLRGSVNIGTITYPV
                     LRLFILGVAIVIAAILFFILNRTRVGAIIRAGVDDREMVSALGINIKQVFTAMFILGS
                     LLAGVAGTLGGSLLNLYPGADSEILTFALVVVIIGGLGTLQGAIAGSLLVGLIDSYGK
                     AYFPELAYFSLFAPMAIILLWRPQGLFGRRSTT"
BASE COUNT          501 a         1004 c         1018 g          400 t
ORIGIN      
        1 gaggtgggcc ccggatcgac caggaccagg tccgcgtccg ctcccggcgc gagcgttccc
       61 ttccgcggcc acaggccgta gcggcgggcc gggccctcgg cgtacgcccg caccacgtcc
      121 tcgagggcca gcttgccccg cagcgcggcg tcgatcagca gcggcagagt ggtgtccagg
      181 ccgggcaggc cgaacgcagc ctcccagatg ccctgggcct tctgctgacg ggtggcgggg
      241 gcgtggtcgg tcgacacgtg ggtgagcacg ccgctgcgga gccggtccca catggcgtcc
      301 tcgtcggcgt cggtacgggc tctggccggc ggcgtgaact tgcggaacgc tcccttctcc
      361 agcacctcgt cctcccgcag gaggaagtac tgcgggcagg cctcggcggc cacgtcggcc
      421 ccggccgcac gctcggcggc gatccgatcc gccaccgcgg gatggctcac gtgggcgatc
      481 gtggcgtggg ccccggtgat cccggacagc agggcggcga cctgggtggc gaccagctcg
      541 gcctcccgcg accgccactg cagcagcacc gccccgtcgt cgcggccggc cttccggagc
      601 gcccgttcgg cctcgccggt gatggtgtcg tcctcgcaat ggatgagcgc cgcggcgcca
      661 gcccgggcca ggtcctcgag cgccgccagg aggtgggcgg cgtcaaggcc ggggaccccg
      721 tgggtggtgc aggtgaagat cttgaagaag gccaccccgg cggcccagag ctcgcccagc
      781 ctcccgatct ggtcgggcca gacgtgggcg gccaggccgt agtccacgtg actccggctc
      841 cgcaggtgcc accgcttgcg ctccagctcc tgcaccgtcc ggaccgggtg gccgtgggtg
      901 tgctcgacga tggtggtgac cccgtgcacc gcggcagccg cggtccccgt gggaaagtcc
      961 tcccgctcgg ttgcgcccgg atccatcaga tgaacgtggg tgtccaccat tccgggcagc
     1021 aggtacaggc cggtggcatc cacggtggtg gcggcatcac gccgctcggg cgagaccgcc
     1081 gccactcgtc cctggtggac gtagaggtgc agccgggccc gtcgcgacgg gcccaccacc
     1141 gtggccccgt ggatgcccag gtcgaactcg ctcatgccac cggggaggaa cgccgctggc
     1201 ggattcgggc gagcatgccg ggcagcttca ggaaggttcc ccagaggccc tcccgcgcga
     1261 acagcaccgt cagcacgaac acgacaccga gcagggtgac ccaccgggtc acgtggatgc
     1321 tcacgtacag ctggatgaac accaccacac ccgctcccac caagggtccc cacagcgtgc
     1381 ccaggcctcc cacgatggcc atcatcacga tgatgccgtt gtgtgagaag tcgatggaat
     1441 tgggcgtgac gtacaggttg tagaaggcgt acaggatccc ggccaccccc gccagggagc
     1501 tggagatgac gaaggccagg tacttgtgca gcgacacgtt gtatcccagg gtgcgcatgc
     1561 gctgctcgtt gtcccggatg ccctgcaggc tcagcccgaa cggcgagcgc acgatggccc
     1621 acagcaacgt ctcgcagagc aggaagatca ccagtacgaa gtagtagagc gtgttggggg
     1681 tgttcaggtc gaaaggcccc aggacgggca gcgagttcac cgaaaggccg ttgtcgccac
     1741 cgctcacact cacccacagc agtgccagac cccacaccag ctggctcagg gccagggtca
     1801 ggatgacgaa cgtcaggtcc cggacccgca ccgccacgac cccgaggatt ccagcggcca
     1861 gcagcaccgc ggccagggcg atggggatcg ccatgttggg gctgatgtgg tggttcgtgg
     1921 cgatggcgat cccgtaggcg ccgatcccca ggaaggcggc gtgtcccagg gagaccagcc
     1981 ccgtatagcc ggccatcagg tcgaggctgg tggccagcag gccgaagatc aggatgttga
     2041 gggccaggga ggccgtgaag gtggatcccc gctgggggat ccatgccgcc acgccgatga
     2101 gcaccaggat ggccgccgcc ttgggccatc gggtgctccg gaccagctgc cgggcttgta
     2161 cggtggtcac gtggtggatc tccgcccgaa cagaccctga ggacgccaca gcaggatgat
     2221 ggccatgggc gcgaacagcg agaagtaggc cagttcgggg aagtaggcct tgccgtagct
     2281 gtcgatgagc cccaccagca ggctcccagc gatcgctccc tgcagggtcc ccaggccccc
     2341 gatgatcacc accaccaggg cgaaggtcag gatctccgaa tcagctcccg ggtacaggtt
     2401 cagcaggctg ccccccagcg tcccggccac ccccgcgagc aggcttccca ggatgaacat
     2461 cgcggtgaac acctgcttga tgttgatccc cagggcgctc accatctccc ggtcgtccac
     2521 gccggcccgg atgatggcgc ccaccctggt ccggttcagt atgaagaaca ggatcgcggc
     2581 gatgacgatg gccacgccga gaatgaacag tcgcagcacg ggataggtga tcgttccgat
     2641 attcaccgac ccccgcaacc acgagggcgt gcggatcgag atggggtcac cgccccagag
     2701 ggacagcgag gagtcgccca tcacgaaggc gatgcccagg gtgagcagca gctcggccag
     2761 gggaaggccc cggagccggg ccagcagacc ccgttccagg acaactccca gcacggccat
     2821 ggccagcgcg gccgcgagca ggcccaagcc gaagttgtgg gtcaccacga tggtggagta
     2881 ggccacatag ccgccgacca ggtagatggc cccgtgtgcc agg
//
LOCUS       DASQQQ010000279         5920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_218356, whole genome shotgun sequence.
ACCESSION   DASQQQ010000279 DASQQQ010000000
VERSION     DASQQQ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218356"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VE646_10515"
     CDS             <1..684
                     /locus_tag="VE646_10515"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZD80454.1"
                     /translation="TRDPDTQEAHRLRELSKEHDLGIGAIHAPFLLMTRSVWGTDPVG
                     KIYRAIELAEDLEVPLVVVHPPYRWQTGYRRWLDERLPDLARHTGVRVAVENMFPVRL
                     RSRRGVSFHTRHQLQDLERFPDVVLDTSHAAVSGLDLEEAFRRLGSRVAHVHLSNNAG
                     RGWDSHLPVQEGVLPLASFLDAIAADGYRGAVSLELDLRRYLDSPGALRDVLVANREF
                     CEARLPDPV"
     gene            736..888
                     /locus_tag="VE646_10520"
     CDS             736..888
                     /locus_tag="VE646_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80455.1"
                     /translation="MRPFTKLILVTLFSLIVVAAVAQLVLARGGHEPYPGPVPGTPWP
                     TVTVSP"
     gene            complement(894..1697)
                     /locus_tag="VE646_10525"
     CDS             complement(894..1697)
                     /locus_tag="VE646_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019871253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HZD80456.1"
                     /translation="MTRPIVFLTDYGLADEFVGVCHGVMARIAPGARVIDLAHHIHRQ
                     DVLHGAVTLARSVRYMPADAVYLAVVDPGVGSARRPVAIEASSGALLVGPDNGVLSMA
                     WGSLGGAVRAVLVTSERVVLRPTSKTFHGRDVFAPAAAHLAAGLPLAELGPPLEPDAL
                     EVVELPAPMVTPGAVGTRVTAVDGFGNVQLNATPAELEAAGLSDRIVIGDRTVPRVST
                     FTDVPRGAPAVIVDSQGFLALVVNHGTAAGLFGLVPGATVKLLRAPLRA"
     gene            complement(1702..2430)
                     /locus_tag="VE646_10530"
     CDS             complement(1702..2430)
                     /locus_tag="VE646_10530"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZD80457.1"
                     /translation="MALVLAVAVITVAAACGDGGGSPASPPSGSRAVTFRSADGVRLA
                     GRLFGTGSTGVVLSHMLPADQSSWWRFAARLADQGYLALTYDFRGYCPGGAAGCSEGS
                     KDVSAIWQDVVGAIGFLSGHGARSVMLVGASMGGTASLVAAARPGIDVAAVVTLSSPQ
                     SIEGLVADADTLSRVRAAKLFVAGNADPTGSASAAENLYRDAPPPKRIQIVTSGDHGT
                     DLLTGSQSGVVSTMILNYLAQYRG"
     gene            complement(2496..2597)
                     /locus_tag="VE646_10535"
     CDS             complement(2496..2597)
                     /locus_tag="VE646_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HZD80458.1"
                     /translation="MPSLVKKRRKKMRKKKHKKLLKRTRWQRRQQGR"
     gene            complement(2621..3511)
                     /locus_tag="VE646_10540"
     CDS             complement(2621..3511)
                     /locus_tag="VE646_10540"
                     /inference="COORDINATES: protein motif:HMM:NF027889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide 5'-hydroxyl-kinase"
                     /protein_id="HZD80459.1"
                     /translation="MNEHDALVDRAVHEKGTVLLVGGLDTGKSTLSRALLAGAVALGR
                     PSAFLDADVGQKSVGPPGTITLKMVRTPADLEPRALVDPDELSFVGSTSPDGHLLQVV
                     TGVGALARRAREAGAEFLVTDTSGLVSGIYGQILKYHKTEVLRPDLVVGLQRGEELLP
                     LLGVIQRFFDTEVVPLGIHPQVVPTTVEQRADNRQGAMGRYFSGELHRWRVKPTVFMP
                     ALPPLFDLSHLDRLLVGLADGAGGYLGIGYLEYAADEGVLRLISPVAEGPKALRLGSV
                     RLEEDYRAKRVDLRHLFGSE"
     gene            complement(3748..4578)
                     /locus_tag="VE646_10545"
     CDS             complement(3748..4578)
                     /locus_tag="VE646_10545"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HZD80460.1"
                     /translation="MDVIASHAAQLPAIDDRWIPRPYATQELVAGLLEGRMAGPVVSH
                     PMDNVLRNIRLLTEDDPDKQFGMSGLAGSMGPSEVLGLVAHAAGFEADPRWVSGPVPV
                     DPALVIEACREVGDRLAFACRRGERVILATGHPVGLAHLYVEVGSLLRDRGVRLLEPA
                     EGVGWWEDGRPHRWQLRYLRSVAMLTDEISARHTHSGEPMHRMLAEARPDLVFADHGF
                     AGAAIEAGVETVSIADVNDPALLVARAQGRTRTVIVMDDNVQPETYWPCFQAIASRLP
                     "
     gene            4605..5393
                     /locus_tag="VE646_10550"
     CDS             4605..5393
                     /locus_tag="VE646_10550"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZD80461.1"
                     /translation="MNDLARAFSLEGKAAVVTGAGRGIGRAIATTFARAGADVVVASR
                     TGEELQRCAAEIRSAGRRAVEVVTDVADPEQVDALIQRAVSELGTLHVLVNNAGAAPF
                     LAPFARTRADGFEKYFDVNFASVVHGMRAAAPVLMAEGEGCVLNIASIDGFMVEPELA
                     YYNAAKAAVISLTKTAALEWADAGIRVNAIAPGWIDTPMNASEREDPEAEERILSQIP
                     MGRWGQPDEIALAALFLCSPASSFITGEVLVVDGGQTVTSARER"
     gene            5396..>5920
                     /locus_tag="VE646_10555"
     CDS             5396..>5920
                     /locus_tag="VE646_10555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZD80462.1"
                     /translation="MDLRGTVVVVTGASSGFGERAAERFAREGAAVVLAARRRERLED
                     LAGRIRERGGRALPVATDVRDTAALQRLRDAVEEGFGRCDVLVNNAGVPGGGWFTSIT
                     LEQIDRIVETNLLGLIRCTKVFLPMLLERGRGHIVNVGSLSGKFALPSATVYSATKHG
                     VIAFSEALSYELEPK"
BASE COUNT          857 a         2141 c         2095 g          827 t
ORIGIN      
        1 acccgggacc ccgacacgca ggaggcgcac aggctgcggg agctctcgaa ggagcacgac
       61 ctggggatcg gggcgatcca cgcgccgttc ctgctgatga cccggagcgt gtgggggacc
      121 gacccggtcg gcaagatcta ccgggcgatc gagctcgcgg aagacctcga ggtacccctc
      181 gtggtggtcc acccgcccta ccgctggcag accggctacc gccggtggct cgacgagcgc
      241 ctgcccgacc tggcccggca caccggggtg cgggtggcgg tggagaacat gttcccggtg
      301 cggttgcgct cgaggcgagg ggtcagcttc cacacccgcc atcagctcca ggatctggag
      361 cggttccctg acgtggttct ggacacgagc cacgcggccg tctcgggcct cgatctggag
      421 gaagcgttcc ggcgcctcgg ctcgcgcgtg gcccacgtgc acctgtcgaa caacgccggg
      481 cggggatggg attcgcacct gccggtccag gagggggtgc ttcccctcgc gagcttcctc
      541 gacgcgatcg ccgccgatgg gtaccggggc gccgtgtcgc tggagctcga tctccgccgc
      601 tacctggact cgccgggtgc gctccgggac gtcctggtgg ccaaccgcga gttctgtgag
      661 gcccggctcc ccgaccccgt ctgaggacag gcctccggcc gccccggccc ggcccgggcc
      721 tgctagcctc ggggcgtgcg cccgttcacg aagctcatcc tcgtcacgct gttctcgctg
      781 atcgtggtcg cggcggtggc acagctcgtg ctggcccgag gcggccacga gccctacccg
      841 gggccggtgc ccgggacccc ctggcccacc gtcacggtct cgccgtaggc ggctcaggcc
      901 cggagcggag ctcggaggag cttcacggtc gcgcccggga ccaggccgaa cagaccggca
      961 gcggtcccgt ggttcacgac cagggccagg aacccctgcg agtccacgat cacggccggc
     1021 gcacctcgag ggacgtcggt gaaggtcgag acccggggga ccgttcgatc cccgatcacg
     1081 atccggtcgc tcaggccggc cgcctccagc tcggcgggcg tggcgttcag ctggacgttg
     1141 ccgaacccgt ccacggccgt cacccgggtc ccgaccgcgc ccggggtgac catgggggcc
     1201 ggcagctcga ccacctcgag cgcgtcgggc tcgagcgggg gaccgagctc ggccagcggg
     1261 agtccggcgg ccaggtgcgc cgcggccggg gcgaacacgt ctcgtccgtg gaaggtcttc
     1321 gaggtcggcc gcaggaccac ccgctccgag gtgaccagca ccgcccgcac cgccccgccg
     1381 agcgagcccc aggccatcga caggacgccg ttgtccgggc ccaccagcaa cgcgccggag
     1441 ctcgcctcga tggccacggg ccgccgggcg gagcccacgc ccggatcgac caccgcgaga
     1501 tacacggcgt ccgccggcat gtagcggacc gatctggcca gcgtcaccgc gccgtggagc
     1561 acgtcctggc gatggatgtg atgggcgagg tcgatcaccc gggcgccggg cgcgatccgc
     1621 gccatcaccc cgtgacacac ccccacgaac tcatcggcca gcccgtagtc ggtcaggaac
     1681 acgatgggcc gggtcaccgg atcagcctcg gtactgcgcc aggtagttca ggatcatcgt
     1741 gctcaccacc ccggattggc tcccggtgag caggtcggtc ccgtggtccc ccgaggtgac
     1801 gatctggatc cgcttgggcg ggggcgcgtc acggtacagg ttctccgcgg cagaggccga
     1861 cccggtgggg tcggcgttcc cggccacgaa cagcttggcc gcccggaccc gggagagggt
     1921 gtcggcatcg gccaccagcc cctcgatcga ctggggcgat gacagggtga ccaccgcggc
     1981 cacgtcgatc ccgggccggg cggcggcgac cagcgatgcc gttccgccca tgctggctcc
     2041 caccagcatc acgctccggg cgccgtggcc ggacaggaac ccgatcgcgc cgaccacgtc
     2101 ctgccagatg gccgagacgt ccttggatcc ttccgagcac ccggcggcgc cgcccggaca
     2161 gtacccccgg aagtcgtagg tgagggccag gtatccctga tccgccaggc gagccgcgaa
     2221 gcgccaccag gagctctgat cggcggggag catgtgggac agcaccacgc cggtggaccc
     2281 cgtaccgaac agccgccctg ccagacgcac cccgtcggcg gaccggaacg tgaccgcgcg
     2341 cgatccggac ggcgggctcg ccggagagcc tccgccgtct ccgcaggccg ccgcgaccgt
     2401 gatcaccgcg accgcgagga cgagggccac ggaccgcggc cgcgcgccgg gtgagcgacg
     2461 tcccgccgct cgcacgatcg cggtctccgg cgctcctagc gcccctgctg acgccgctgc
     2521 cagcgggtcc tcttcaggag cttcttgtgc ttcttcttcc tcatcttctt gcggcgcttc
     2581 ttgacgagcg acggcacgcg gttcccttct gctcagcctg ctactcggat ccgaacaggt
     2641 gccggaggtc gacccgcttc gcacggtagt cctcctccag acggacggag cccagtctaa
     2701 gggccttggg cccttctgcc accggagaga tgagccgcag caccccctcg tcggcggcgt
     2761 attccaggta gccgatcccc aggtagccgc cggcaccgtc cgccagaccc accagtagcc
     2821 ggtcgagatg ggacaggtcg aacagcggcg ggagcgccgg catgaagacg gtgggcttga
     2881 cccgccaccg atgcagctcg cccgagaagt atcggcccat ggcgccctgt cggttatcgg
     2941 ctcgttgctc gaccgtggtg ggcaccacct ggggatggat cccgagcgga accacctcgg
     3001 tatcgaagaa gcgctggatc acgcccagca gtgggagcag ctcctcgccg cgctgcagcc
     3061 ccaccaccag atcgggacgg agcacctcgg tcttgtggta cttgaggatc tgcccgtaga
     3121 tgccggacac gagccccgac gtgtccgtga ccaggaactc ggcccccgcc tcgcgcgccc
     3181 ggcgggccag ggcccccacg ccggtcacga cctggagcag gtgcccgtcg ggggaggtgg
     3241 agcccacgaa ggaaagctcg tcggggtcga ccagggctcg gggctccagg tcggcgggcg
     3301 tccggaccat cttcaacgtg atcgtccccg gagggccgac cgacttctga ccgacgtcgg
     3361 cgtcgaggaa ggccgagggg cggccgaggg ccaccgcccc tgccagcagg gcccgggaca
     3421 gcgtgctctt gcccgtgtcc agcccgccga ccagcagcac ggtgcccttc tcgtgcaccg
     3481 cccgatcgac gagcgcgtcg tgctcgttca cttccgcctc cgcggggtcg atcccctcgg
     3541 agacccgctc cggtgcgtgc gtgaccgatc tatcccgagg gtcgatcccc ccgttcgcct
     3601 ggtcgctcga cggacatggt catcgggaat gagaggtcca cgacctcctt cacgcggttg
     3661 gaaccgagcc tcacgagagc ggttctcgtc gtcatcgggg ccgattgtac ccgcaggcgt
     3721 ggccgcacct gatcctgacc gccaggttca gggcaggcgg gaggcgatgg cctggaagca
     3781 gggccaatac gtctcgggct ggacgttgtc gtccatcacg atgacggtcc gggtccgacc
     3841 ctgggcccga gccaccagca gggcggggtc gttcacgtcg gcgatcgaga cggtctcgac
     3901 gccggcctcg atcgcggccc cggcgaaccc gtggtcggcg aagaccaggt ccggccgcgc
     3961 ctcggccagc atgcggtgca tcggctctcc gctgtgcgtg tgccgggctg agatctcgtc
     4021 cgtcagcatg gccaccgacc gcaggtaccg cagctgccat cgatggggtc gcccgtcctc
     4081 ccaccacccc acgccctcgg ccggctcgag gagcctcacg ccgcgatccc ggagcaggct
     4141 ccccacctcg acgtagaggt gggccaggcc gaccgggtgc ccggtggcca ggatcacccg
     4201 ttcgccgcgg cgacaggcga acgcgagtcg gtcccccacc tcccggcacg cctcgatcac
     4261 cagcgcggga tccaccggca ccggcccgct cacccatcgg ggatcggcct cgaaccccgc
     4321 ggcgtgggcc accagcccca ggacctcgga gggccccatc gatccggcca gcccgctcat
     4381 cccgaactgc ttgtcgggat cgtcctcggt gagcaagcgg atgttgcgga gcacgttgtc
     4441 catcggatgg ctcaccaccg ggccggccat ccgtccctcc agcaggccgg cgaccagctc
     4501 ctgggtggcg tacgggcggg ggatccagcg atcgtcgatg gccggcagct gcgctgcatg
     4561 tgatgcgata acgtccatca catccatcgt acgggagcga ccctatgaac gacctcgcac
     4621 gcgcgttctc gttggagggc aaggcggccg tggtgaccgg cgctggccga gggatcggcc
     4681 gcgccatcgc cacgaccttc gcccgggccg gtgccgacgt ggtggtggcc tcccggaccg
     4741 gggaggagct gcagcgctgc gccgccgaga tccgctcggc cggccgccgg gcggtggagg
     4801 tggtcaccga cgtcgccgac cccgagcagg tcgatgcctt gatccagcga gcggtgagcg
     4861 aactcgggac gctccacgtg ctggtgaaca acgcgggggc cgcgccgttc ctggccccgt
     4921 tcgcccggac ccgggccgac gggttcgaga agtacttcga cgtgaacttc gcctcggtgg
     4981 tccacggcat gcgggcggcc gccccggtcc tgatggccga gggcgagggc tgcgtgctga
     5041 acatcgcgtc gatcgacggg ttcatggtgg agcccgagct cgcctactac aacgcggcca
     5101 aggcggcggt gatcagcctg accaagacgg cggcgctgga atgggccgac gcggggatcc
     5161 gggtgaacgc gatcgccccc ggatggatcg acacgccgat gaacgcctcc gagcgcgagg
     5221 accccgaggc cgaagaaagg atcctgtcgc agatcccgat gggccgctgg gggcaaccgg
     5281 acgagatcgc cctggccgcg ctgttcctgt gctcgccggc ctcctccttc atcaccggcg
     5341 aggtcctggt ggtggacggc ggccagaccg tcacgagcgc ccgggagcgc tgaacgtgga
     5401 tctgcggggc acggtcgtgg tggtcaccgg cgcctcgtcc gggttcgggg agcgcgccgc
     5461 cgagcgtttc gcccgggagg gggcggcggt ggtgctcgcg gctcggcggc gggagcggct
     5521 cgaggacctg gccgggcgga tccgggagcg cggcggccgg gccctgccgg tggccaccga
     5581 cgtgcgggac acggccgccc tgcagaggct gcgagatgcc gtcgaggagg ggttcggacg
     5641 ctgcgacgtg ctggtcaaca acgccggggt tcccggcggc gggtggttca cgagcatcac
     5701 cctcgaacag atcgaccgga tcgtcgagac gaacctgctc gggctgatcc ggtgcacgaa
     5761 ggtcttcctg cccatgctgc tcgagcgtgg acggggccac atcgtgaacg tgggctccct
     5821 ctccgggaag ttcgccctgc ccagcgcgac cgtctacagc gccaccaagc acggggtcat
     5881 cgcgttctcc gaggccctgt cctacgagct cgagccgaag
//
LOCUS       DASQQQ010000280         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_48223, whole genome shotgun sequence.
ACCESSION   DASQQQ010000280 DASQQQ010000000
VERSION     DASQQQ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48223"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..629)
                     /locus_tag="VE646_10560"
     CDS             complement(168..629)
                     /locus_tag="VE646_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HZD80463.1"
                     /translation="MDVEALVRPVVESAGLELWDAVFRKEGGRMVLRVAVDRDGGVDL
                     DTISQVSERLSRRLDLEDFGRGRYELEVSSPGLERALRRPRHFQRSVGERVRVKTVQP
                     VNGTKTHEGALVSADAEAIVIASEGGELRVPYDAVARARTIFEWGSARSNT"
     gene            complement(756..1037)
                     /locus_tag="VE646_10565"
     CDS             complement(756..1037)
                     /locus_tag="VE646_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80464.1"
                     /translation="MAVRPPWELQRFVMLHPEAEGVIQHVGRATWDLVVVDVDGTWVR
                     AEFASAEEAEAACRILGIRSNRGWDDPRLCRRMARRDHWNAPGGQRRAL"
     gene            complement(1055..>2034)
                     /locus_tag="VE646_10570"
     CDS             complement(1055..>2034)
                     /locus_tag="VE646_10570"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HZD80465.1"
                     /translation="SQDGLGPLRPVDTPGAATIEEVSTFLGLPSERMLKTMLYDAGGR
                     TLAVLVPGDREVEEAKIERAFFPTTIRQLQEEEFERRGFVKGFVGPQGLGQDVTIVAD
                     PSVRGGVDWVTGANQTDRHVTGANTPRDFRVDRFADVVALREGDRCPSCGGTLHVGRA
                     IVVGHIFQLGTNYSKPLDARFVDEDGQERHYLMGSYGIGISRILAGAVEQFHDQGGLR
                     LPKGLAPFEVVVVAANRDDPQVTAEAERIYGELRERGVEAVIDDREQTAGVKFADADL
                     VGYPVQVVVGRRGVRDGVVDLKLRSSGERSTAPLAQAAQAAQALLASAP"
BASE COUNT          283 a          822 c          655 g          274 t
ORIGIN      
        1 gatcttggcg cgcacgccga aggactcctc ctccacctcg gggtgctgct gccgccacca
       61 ggacttgtag gcggaggcca tggcctcctc cagcccggcc aggatcgtct cgaaggcgat
      121 ccccttctct cgctccagct cgcggagcgc tgcgatcatg tccggattca tgtgttgctc
      181 ctcgccgaac cccactcgaa gatggtccgg gcccgggcca cggcgtcgta cggcacgcgc
      241 agctctccgc cctcggaagc gatcacgatc gcctccgcat cggccgagac cagcgcgccc
      301 tcgtgggtct tcgtcccgtt caccggctgc accgtcttca ccctcacccg ctcccccacg
      361 ctgcgctgga agtgccgggg ccgccgcagc gcccgctcca gcccgggcga gctcacctcg
      421 agctcgtacc gtccccggcc gaagtcctcc aggtccaggc gccgcgagag ccgctccgac
      481 acctgcgaga tggtgtccag gtccacgccg ccgtcccggt ccacggcgac gcgcaagacc
      541 atccggccac cctccttgcg gaacacggcg tcccacagct ccaggccggc cgactcgacc
      601 accggccgga ccagcgcctc aacgtccatc tcgcctgcct cgccgacgcg gccccaccga
      661 aaaagaaagt gggcgcgagc ccacttccgc gtgaagccag tcccgattgc ggttcgagta
      721 taccaacggc gccgccggca ccccgccgcg ggggctcaga gggcgcggcg ctggccgccg
      781 ggcgcgttcc agtggtcgcg acgggccatg cgccggcaca ggcgggggtc gtcccagccc
      841 cggttggagc ggatccccag gatccggcac gcggcctccg cctcctcggc agaggcgaac
      901 tcggcccgga cccaggtgcc gtccacgtcc accaccacca ggtcccaggt ggcccgcccc
      961 acgtgctgga tcacgccctc cgcctcgggg tgcagcatca cgaaccgctg gagctcccag
     1021 gggggccgaa ccgccatgga gcaagcgtag cccgtcaggg ggccgacgcc aggagcgcct
     1081 gggccgcctg ggccgcctgg gccagcgggg cggtggagcg ctcccccgag gaccgcagct
     1141 tgaggtcgac cacgccgtcc cgcacgcccc ggcgccccac caccacctgc accgggtagc
     1201 cgaccaggtc ggcgtcggcg aacttcaccc cggcggtctg ctcgcggtcg tcgatcaccg
     1261 cctccacgcc gcgctcgcgc agctcgccgt agatccgctc ggcctcggcg gtcacctggg
     1321 ggtcgtcccg gttggccgcc accaccacca cctcgaacgg ggccaggccc ttgggcagac
     1381 ggagcccccc ctggtcgtgg aactgctcca cggcgccggc caggatccgg gagatcccga
     1441 tgccgtagga gcccatcagg tagtggcgct cctgcccgtc ctcgtccacg aaccgggcgt
     1501 ccagcggctt ggagtagttc gtccccagct ggaagatgtg gcccaccacg atggcccggc
     1561 ccacgtgcag ggtccccccg cagctcggac agcggtcgcc ctcgcgcagg gccaccacgt
     1621 cggcgaatcg gtccacccgg aagtcccggg gggtgttcgc cccggtgacg tgccggtcgg
     1681 tctggttcgc gccggtgacc cagtccaccc caccgcggac cgacggatcg gccacgatcg
     1741 tcacatcctg ccctaggccc tgcggtccca cgaagccctt cacgaacccg cgccgctcga
     1801 actcctcctc ctggagctgg cggatcgtgg tggggaagaa ggcccgttcg atcttggcct
     1861 cctcgacctc gcgatcgccg gggaccagga ccgccagggt ccggcccccg gcgtcgtaca
     1921 gcatcgtctt cagcatccgc tccgagggca ggcccaggaa ggtcgagacc tcctcgatcg
     1981 tggccgcccc cggcgtgtcc acgggccgca gcggcccgag cccgtcctgc gagg
//
LOCUS       DASQQQ010000281         2445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_131849, whole genome shotgun sequence.
ACCESSION   DASQQQ010000281 DASQQQ010000000
VERSION     DASQQQ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131849"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VE646_10575"
     CDS             <1..393
                     /locus_tag="VE646_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZD80466.1"
                     /translation="MIQEAEAHAAEDKKRRERVEIRNQADALAYQVEKGLKDLGDKIP
                     ADEKAKVEASLERTREALKHDDEAELKAALEELRGRWSEATTHAYEAAGAAEESSAAG
                     GPQPEPGEAEAPEEDVIEADYEVVDEEK"
     gene            559..2082
                     /locus_tag="VE646_10580"
     CDS             559..2082
                     /locus_tag="VE646_10580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZD80467.1"
                     /translation="MVQPGAPPHTLYPPGYPALLVPLYLISGGSLVAMKLLSLAAGAV
                     ALWATYRLARRDPAVPAWAAAASVPLVGLYPAFREYSHWVLSDMTYTALAVAALLVLQ
                     GEADRGDGRPRWGRWLLACGLALGAVYVRAAGVALVAATIGWALLRRRWKPAAGAALL
                     FAVGLLPWIAWSRQSAPTAPAGYLDQAVAASPFSGPPQELLQALGEQFRDVVVEYGTY
                     QLPELFWPATPPPPAARALGAILGGVILLFGVWRVIRGRRLRPWDLYVAATVCLIYLW
                     PWLGERIFLTLAPFLWLYLLAGADDLSRRLGAGRAPAMAAAGVVALALLMGGVADATR
                     QIPRTRAYLAGDRLGGYPAFWQDYFEAARWIGANVPEAVILARKPTLAWYWSGRPAVV
                     YPRGADPPETWRFIRQAGVTHILFEHSTEEALAPTLARSRARLELVHRAPQGRAAVVR
                     LGSTGPTSIERKATRSSFLRVATPDRGSPGTRPGGAGTRSGGDRIRRHGQVESRLIV"
     gene            2108..>2445
                     /locus_tag="VE646_10585"
     CDS             2108..>2445
                     /locus_tag="VE646_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80468.1"
                     /translation="MHPIRTKLAVLAVGLACLALGVLFASTVDHTTGTLATAQTGQTV
                     PTTESSLRETQNAFVGIAQKVTPSVVFVTGQRRVEAPDMQFDLRPFEDFFDFRGPHSQ
                     PGPQYRPSGGS"
BASE COUNT          342 a          891 c          840 g          372 t
ORIGIN      
        1 atgatccagg aggccgaagc ccacgcggcc gaggacaaga agcgccgcga gagggtcgag
       61 atccgcaacc aggccgatgc cctggcctac caggtcgaga agggcctgaa ggacctcggg
      121 gacaagatcc ccgccgacga gaaggcgaag gtcgaggcct ccctggagcg cacccgcgag
      181 gcgctcaagc acgatgacga agccgagctg aaggcggccc tcgaggagct ccgcgggcgc
      241 tggagcgagg ccacgaccca cgcctatgag gcggctggcg cagccgagga gtcttcggcg
      301 gcgggcggcc cgcagcccga gcccggcgag gccgaggcgc ccgaggagga cgtcatcgag
      361 gccgactacg aggtggtcga cgaggagaag tagggcgtcg accgctccga ttccccccga
      421 cgatgggcga ttgccggcgg agccctcgcg ctcgcgctca ccatcaccgc cttcgatccc
      481 tatctgttca ccgggggcga caacgcccag tactacgcac tgacccgcgc cctggcgacg
      541 gggcgcgggt acgtcgacct ggtccagccc ggcgcgcccc cccataccct ctatccgccc
      601 ggctatccgg cgctcctcgt gccgctgtac ctgatcagcg ggggaagcct tgtcgccatg
      661 aagcttctct ccctggcggc cggggccgtg gccctgtggg ccacctaccg gctggcgcgc
      721 cgcgatccgg cggtccccgc gtgggcggcg gccgcctcgg tcccgctcgt ggggctctat
      781 cccgcgttcc gggagtattc gcactgggtg ctgtcggaca tgacgtacac cgcccttgcg
      841 gtcgccgccc tcctcgtcct gcagggcgag gccgaccgcg gcgacgggag accacgatgg
      901 gggaggtggc tgctggcctg cggcctggcg ctcggggccg tctatgtacg ggccgccggt
      961 gtggcgctgg tggcggcgac catcggctgg gccctcctgc ggcggcgctg gaagcccgcc
     1021 gcgggggccg cgctcctctt cgcggtcggt ctgctcccct ggatcgcgtg gtcgcggcag
     1081 tcagcaccga cggccccggc cggttatctc gaccaggcgg tggccgcctc gccgttttcg
     1141 ggcccccctc aggagctcct ccaggccctc ggcgagcaat tccgcgacgt cgtcgtcgag
     1201 tacggcacct atcagctgcc ggagctgttc tggccagcca cgccacctcc acccgccgcc
     1261 cgcgccctgg gagcgatcct agggggtgtg atcctgctgt tcggcgtctg gcgggtaatc
     1321 cgagggcgca ggctccggcc ctgggatctg tacgtcgcgg cgacggtctg cctcatctat
     1381 ctctggccct ggctgggcga gcggatcttc ctcaccctgg cccctttcct gtggctctat
     1441 ctgcttgccg gagccgacga cctgtcgcgg cgtctcggcg ccggccgggc cccagcgatg
     1501 gccgccgccg gagtggtcgc gctggcgctc ctgatgggcg gcgtggccga cgcgacccgg
     1561 cagatcccac ggacgcgggc ctatctggcc ggggatcggc taggcggcta ccccgccttc
     1621 tggcaggact acttcgaagc cgcgcgatgg atcggcgcca acgtccccga ggccgtcatc
     1681 ctcgcgcgca agcccaccct cgcctggtac tggagcggac gcccggcggt cgtctacccg
     1741 cggggtgccg atccccccga aacctggcgt ttcatccgcc aagccggcgt cacccacatc
     1801 ctgttcgagc actccaccga ggaagcgctg gcaccgacgc tagcgcggag tcgggcccgg
     1861 ctcgaactgg tgcatcgcgc cccgcagggt cgagcggcgg tcgtccgtct cggctcgacc
     1921 ggcccgacct ccattgaacg gaaagcgacc cggtcatcgt ttctgagggt ggcgacgcca
     1981 gaccgggggt caccgggaac ccgacccgga ggtgcgggaa cccgatccgg aggtgaccgt
     2041 ataagaagac atggacaggt cgaatcccgg ctgatagtct gagtgatctg acaggaggtt
     2101 ccatcccatg caccccatca gaaccaagct cgccgttctg gccgtcggtc tggcctgtct
     2161 ggcgctgggt gttctgttcg cgtctaccgt ggaccacacg accggcaccc tggccaccgc
     2221 ccagaccggg cagacggtgc ccaccacgga gagctcgctg cgcgagacgc agaacgcgtt
     2281 cgtcggcatc gcccagaaag tcaccccttc ggtcgtcttc gtgaccggcc agcgccgcgt
     2341 cgaagccccg gacatgcagt tcgatctcag gcccttcgag gatttcttcg atttccgggg
     2401 gccccactcc cagccgggtc cccagtaccg gccgagcggg ggctc
//
LOCUS       DASQQQ010000282         2675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_266976, whole genome shotgun sequence.
ACCESSION   DASQQQ010000282 DASQQQ010000000
VERSION     DASQQQ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266976"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VE646_10590"
     CDS             <1..997
                     /locus_tag="VE646_10590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZD80469.1"
                     /translation="RDTSAKQLAALLGEGMAAACHDAAELMPVPFEIRAEASGADRLS
                     VAERLDRTGLRAEVRDLLRGGLGTLSSAPLAEAGYVPTALKAFALASSDAGVFLEQNA
                     SHTIRGGTRALVEAMAADARADVALSTDVVSVRRSADAVTLTANGTQMRARAAVMAVP
                     LNALRRIDFDPPLSAAKRRVASAGQASRGAKLFLRIRGHHAPLMILAPEEHHLTLLEW
                     AGTIEDDTLLVGFASTNRVLSDRGLLGRAVADLLPGAEVLDVLAHDWTSDPLAGGTWC
                     TLRPGQFTGALQELRRSEGRLVFAGGDIAAGWLGYIDGAIETGLSAARQVRALLS"
     gene            1194..2597
                     /gene="glnA"
                     /locus_tag="VE646_10595"
     CDS             1194..2597
                     /gene="glnA"
                     /locus_tag="VE646_10595"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HZD80470.1"
                     /translation="MAKEAHVQIVDLRFCDLPGLMQHFSIPVTELSEDSFSDGLGFDG
                     SSIRGFQEIQESDMLLVPDPDTAYLDPFTKHATLNVNCFVKDPITGEMYTRDPRYIAR
                     KAELYLKQTGIADTSFWGPECEFYIFDAIRFDQNQHEGYYHLDAIEGVWNSGRERELS
                     GEPNLGYKPRYKEGYFPLPPMDHYQDLRSEMVLAIQKVGIQIEVHHHEVGTAGQAEID
                     IRYDTLLTTADKVMKYKYVVKNTARAFGKTVTFMPKPIFMDNGSGMHTHQSLWKNDDN
                     LFWDEVGYAGISDTARWYIGGLIAHAPGLLAFTNPTTNSYRRLVPGYEAPINLVYSQR
                     NRSACVRIPQYFKNPAAKRLEFRTPDPSSNPYLAFSAMLMAGLDGIKNKIEPPEPIDK
                     DLYDLPPEEKSSVKQVPGSLDEVLTALEADHQWLLEGGVFTQDVIDTWIEYKRLHEVD
                     QVRLRPHPHEFAMYFDL"
BASE COUNT          454 a          943 c          885 g          393 t
ORIGIN      
        1 ccgagacacc agcgccaagc agctcgcggc cctgctgggc gaaggcatgg ctgccgcctg
       61 ccacgatgcg gccgagctca tgccggtccc cttcgagatc cgtgccgagg catccggagc
      121 cgaccgcctc agcgtggccg agcgcctgga ccgcacgggc ctccgcgccg aggtgcggga
      181 cctgctccga ggggggctcg gcaccctgtc gagcgcgccg ctcgccgagg ccgggtacgt
      241 gcccaccgcg ctcaaggcat tcgccctcgc cagttcggac gcgggggtct tcctggagca
      301 gaacgcgagc cacacgatcc ggggagggac ccgcgccctc gtcgaggcca tggccgccga
      361 cgcccgggcc gacgtcgccc tctccacgga cgtggtgtcg gtgcgccgat cggccgatgc
      421 ggtcacgctc accgcgaacg ggacgcagat gcgggctcga gccgccgtga tggccgtgcc
      481 cctgaacgcc ctgcggagga tcgatttcga tcccccgttg tcggccgcca agcggagggt
      541 cgcctcggcg ggacaagcct cccgaggagc caaactcttc ctgcggatcc gcgggcatca
      601 cgcccccctc atgatcctgg cgcccgagga gcaccacctc accctgctgg agtgggccgg
      661 aacgatcgag gacgacaccc tgctggtggg cttcgcgtcc acgaaccggg tgctgtccga
      721 ccggggactg ctgggccggg cggtggccga cctccttccc ggggccgagg tcctcgatgt
      781 actggcccac gactggacgt ccgatccgct ggccggcggg acctggtgca cgcttcgacc
      841 cggtcagttc accggcgccc tgcaggagct gcggcggtcg gagggccggc tggtcttcgc
      901 gggcggggac atcgcggcgg ggtggctcgg ctacatcgac ggggctatcg agaccggcct
      961 cagcgcggct cgccaggtcc gggcgctcct ctcatgaggc cgtccagggc gtcgcggcgc
     1021 cgtcccgtcg gcccatgtcc ttccgccggt cgaagaggca gcggtgggcc cgctgacgta
     1081 acgaggaatt aacggcggac gagccgcgac gcctcggccc gggactagcc tggcagggtc
     1141 gtccttcgag gcaaaggggg aagcacgtgg cgacgaccaa ggaagtcctg gccatggcca
     1201 aggaggccca tgttcagatc gttgacctgc ggttctgcga tctgcccggg ctcatgcagc
     1261 acttctcgat cccggtcacc gagctgtccg aggacagctt ctccgacggc ctggggttcg
     1321 acggttcatc gatccgcggg ttccaggaga tccaggaatc ggacatgctc ctggtccccg
     1381 accccgacac cgcctacctg gatccgttca ccaagcacgc caccctgaac gtgaactgct
     1441 tcgtgaagga cccgatcacg ggcgagatgt acacacgcga cccgcgctac atcgctcgga
     1501 aggccgagct gtacctgaag cagaccggca tcgccgacac gtcgttctgg ggaccggagt
     1561 gcgagttcta catcttcgat gcgatccgct tcgaccagaa ccagcacgag ggctactacc
     1621 acctcgacgc gatcgagggc gtgtggaact ccggacgcga gcgcgagctc tccggtgagc
     1681 cgaacctcgg ctacaagccc cgctacaagg agggctactt ccccctgccc cccatggacc
     1741 actaccagga cctccggagc gagatggtgc tggccatcca gaaggtcggg atccagatcg
     1801 aggtgcacca ccacgaggtg gggacggccg gccaggcgga gatcgacatc cgctacgaca
     1861 cgctgctcac caccgccgac aaggtcatga agtacaagta cgtggtcaag aacacggccc
     1921 gtgccttcgg caagaccgtg acgttcatgc ccaagccgat cttcatggac aacggctcgg
     1981 gcatgcacac ccaccagagc ctgtggaaga acgacgacaa cctgttctgg gacgaggtgg
     2041 ggtacgcggg gatctcggac acggcccgct ggtacatcgg cggtctgatc gcgcacgcac
     2101 cgggcctgct ggccttcacc aaccccacca cgaactcgta ccggcgattg gtgcccggct
     2161 acgaggcgcc catcaacctc gtgtactcgc agcggaaccg cagcgcgtgc gtccggatcc
     2221 cccagtactt caagaacccg gcggccaagc gcctggagtt ccggaccccc gatccctcct
     2281 ccaacccgta cctggcgttc tcggccatgc tgatggccgg cctggacggg atcaagaaca
     2341 agatcgagcc gccggagccc atcgacaagg acctgtacga ccttcccccc gaggagaagt
     2401 cgtcggtcaa gcaagtgccg ggctcgctgg acgaagtgct gaccgccctg gaggccgacc
     2461 accagtggct gctcgagggc ggggtcttca cccaggacgt gatcgacacc tggatcgagt
     2521 acaagcggct ccacgaggtg gaccaggtgc gcctgcggcc gcacccgcac gagttcgcga
     2581 tgtacttcga tctgtaggcg ttgacccgct ccatccggcc cgcggccccg atcgacctcg
     2641 gggagacgct gcgaccattg tggcggggcc ccggc
//
LOCUS       DASQQQ010000283        13989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_96245, whole genome shotgun sequence.
ACCESSION   DASQQQ010000283 DASQQQ010000000
VERSION     DASQQQ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13989
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96245"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VE646_10600"
     CDS             complement(<1..1007)
                     /locus_tag="VE646_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005087598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HZD80471.1"
                     /translation="MRPLDRLPTIRWKIASTLVIAVGITVVLIFLMLGYALRGTSQNA
                     DRLALLSAARRYATGGLARVPEHVSLVTVGVDGQLLSGTAPGRLPRFTDGLVHVGSTR
                     ELQYAVVPVVKQGVLDHLVYAVRPAQGRGLLDRLTATFRFLFDFWWQFLLAGALAAAV
                     GLGLARWLARGMTQPLRDMANAARIMETGDYSHRVHTASRDEVGQLTAAFNRMSAELE
                     SLEQLRRDLVANVSHELKTPISALRAHLENLLDGVEEPHPETLQVMLSQSDRLGRLVD
                     RLLDLSRLESGELPLQRVAVSLASLTREVLPEIEVARSDRGVAIEDLVPADLPPVLAD
                     R"
     gene            complement(1004..1705)
                     /locus_tag="VE646_10605"
     CDS             complement(1004..1705)
                     /locus_tag="VE646_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014351486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD80472.1"
                     /translation="MAQQTVLVVDDEEAIAEAVRARLESEGYRVVVAADGPQAIQAAA
                     DEGPDLVVLDLMLPGMDGLEVCTEIQRERWVPVLMLTARTEEADKVAGFAVGADDYLT
                     KPFSLRELVMRVRAILRRVDRIRASGEMEPLERGALTVDASRRRVFVDGQEVALTPLE
                     FEILLTMARDPGVVFTREQLMDRVWGYRDYAGGRVVDSHVARIRRKLGEDGAEPRFIR
                     TVHGVGYAFQDRPGS"
     gene            complement(1790..2902)
                     /locus_tag="VE646_10610"
     CDS             complement(1790..2902)
                     /locus_tag="VE646_10610"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HZD80473.1"
                     /translation="MAGTVGEIAFFVALILVILVHEAAHFGVAKRFGFKVDEFFVGFG
                     PRIWSTRRGETEYGVKWIPAGGYVRIAGMNPFEQVRPEDVPRTYGSKPIWQRALVIAA
                     GPSTHFVLAFLLFAIWLGMVGQPSARSPLVAEVAPRLAGHPSPAATAGLRAGDQIVAI
                     DGISHPTDAQLIRFTRRHVGQEIALQVERDGRVLTVHLTPVLSQVGGRQVGRIGVALT
                     GTRETAGPLASITGGGGMVWDSLVGSVKGLGRLFGPEGLGRLGRLLFTNAPRSSSDVQ
                     SVVGVGRVVGQAAAGSRFWDILWVFAFVNVFIGFLNLLPLPPFDGGHLAVLAVEKARG
                     RKIDMRKLIPISAAVATLFVLLFVAVVYLDIAKPVG"
     gene            complement(2916..4082)
                     /gene="dxr"
                     /locus_tag="VE646_10615"
     CDS             complement(2916..4082)
                     /gene="dxr"
                     /locus_tag="VE646_10615"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016909836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HZD80474.1"
                     /translation="MRSLIILGSTGSVGTQALDVVRRNAERFKVVGLSAGTNHELLVG
                     QIREFLPPFVAMADEAAAGELREMLASGTTLPNVEILVGPDAAERLARDVEADMVVNA
                     LVGAAGLAPTLAALQSGKVLALANKESLVIGGELVTDLIKGEPERLVPVDSEHAALAQ
                     LLRGERREEVKRVIVTGSGGPFRGWTRSELARASVKEALAHPVWQMGPKITIDSATLM
                     NKGLEVIEAHYLFNIEYPKIHVVVHPEGIVHGLAEFRDGTMLAQLAVPDMRLPIQLAL
                     AWPERLPSGVEPVPLADRKLTFEPVDREAFPAIDLAYRVGELGLTFPAVMSAANEVAV
                     MAFLEGKIPLTRIVEVIQGVVDEHEAPASVVSVVNLERADGWARRRAAEIIEAR"
     gene            complement(4174..5706)
                     /locus_tag="VE646_10620"
     CDS             complement(4174..5706)
                     /locus_tag="VE646_10620"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZD80475.1"
                     /translation="MPEGENHNDLFEDLDKFFAPIQDVDWPETSPPRRERTTEEQGAP
                     EEPVRIPEEEPPAGPDAEGEGLFGEAETAGAEDQGAVRGAEPEEEEEELLDEGSAPTD
                     LDIAEAPVPEPISVESFTRPPDAYVDLPGMDEEIESATPAELDEGEEAPTPEAVEAAA
                     EHFAASVRDEAITEPGSEPQPVGTDVPTTTLPDLFGDEEEPTEEELPPVRAVRVGAEG
                     LGGPSWQEPTAVEVGADQEPRRTGRNVPAAFLTGLALALLAGGSIAIGKGAFAIVAGV
                     IVLWAQGELYRALQKEHHQPATALGLVSGGLVLAGAYFRGEAAMLSMFVLSIFATFLW
                     YMTVPASHRRGAAGNLGLTVLGVAYVPLLAGYALTLFRFPEGRAVTISVIGLAFLYDT
                     AAFAVGSLWGSRPLASTVSPKKSVEGAIGATLVVIAVAIGVVPSSVAILDTIPRAAGL
                     ALVVAVFALLGDLAESLLKRDLGLKDMGSILPGHGGVLDRIDSILFVVPAAYFFLRLI
                     LL"
     gene            complement(5706..6227)
                     /gene="frr"
                     /locus_tag="VE646_10625"
     CDS             complement(5706..6227)
                     /gene="frr"
                     /locus_tag="VE646_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011247082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HZD80476.1"
                     /translation="MTKAISVLREELASIRTGRATSALVSRTTVDYYGTQVPLQQLAS
                     FSVPEPRTLLIQPYDKNAISAMEKAILASDLGITPSNDGAVIRLSFPPLTEERRKDLI
                     KLVHQRGEDGRVAVRNVRRHAKDELEKLERDHAISEDDLMRSEKELQKLTDQHVAEVD
                     ETIAHKEQELKEI"
     gene            complement(6263..7012)
                     /gene="pyrH"
                     /locus_tag="VE646_10630"
     CDS             complement(6263..7012)
                     /gene="pyrH"
                     /locus_tag="VE646_10630"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZD80477.1"
                     /translation="MAEADPQRPPSRPLFHRVLLKLSGDAFAPSDTGYGISAEATGLL
                     ARQLVEVLDLGVQTAVVVGGGNIFRGRQAPDIERSRADYMGMLATVMNALALQDALEK
                     IHATTRVQTSIHMAQIAEPYIPLRAIRHLEKGRIVIFAAGLGAPYFSTDTTAAQRALE
                     IHADAILKGTKVDGVYDADPHLNPAAKRFESIEYIDVLREGYQVMDATAISLCMENDL
                     PIVVFDFGREGNIRRAVSGESIGTLVHGRAS"
     gene            complement(7023..7631)
                     /gene="tsf"
                     /locus_tag="VE646_10635"
     CDS             complement(7023..7631)
                     /gene="tsf"
                     /locus_tag="VE646_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZD80478.1"
                     /translation="MVDIGVDLIRQLRERTGAGMMDCKRALQEAEGDMDRAFEILRER
                     GQASVSKRAGRVANQGRVASYIHFNHTVGSLVEVNCETDFVANTDEFGGLAKDIALHV
                     ASPAAPRWLSREDVPPDVLEQERHVFEAQAREMGKPDDVVPKIVEGKLEAFYKDHVLL
                     DQPFVRDDGRTIRQLLDEVGAKVGEKVAVRRFVRYKLGEDGD"
     gene            complement(7631..8476)
                     /gene="rpsB"
                     /locus_tag="VE646_10640"
     CDS             complement(7631..8476)
                     /gene="rpsB"
                     /locus_tag="VE646_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019929960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZD80479.1"
                     /translation="MPVVTRRELLEAGIHFGHQTRRWNPKMGRYLYGERSGIYIIDLE
                     KSLVGIEEAYAFVRDLSRRGGIVLFVGTKKQAQEVIAEEAARVGMPHVTTRWLGGMLT
                     NFQTVSRRLLRLRELREMERGGAFDFLPKKEVLKLRHEKEKLERNLGGMQDLERLPDA
                     IFVIDTKKEHIAVNEARKLGVPVVAIVDTNCDPDEVDYVIPGNDDAIRSVALVTRVVA
                     DAIAEGRRIGREERVTRASGPEIEPEPGTPAPRPEAAAPSSAEAPDGTTAPAADASAG
                     PKDGV"
     gene            complement(8608..9615)
                     /locus_tag="VE646_10645"
     CDS             complement(8608..9615)
                     /locus_tag="VE646_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80480.1"
                     /translation="MSKKRRRRSGPGSRGTNGGPGRSSPTLATKERERPERTEQRRSE
                     PTGIMGWLARGTGESRLPSISRSLGRGLLIVASSPVVLGSTLLFVLLGWLGMVTLGLE
                     GPVGRLVYMTALPPIGTYWDTYNGVAIFGFGIAGLAWGAAFLAVRALFVSVMTGLVVQ
                     ELEGEGGPVEGALRGVRAFPIVLLVNVLAMSLMITGTIILPFLGPGLGFFGSVAILVA
                     AMFFFVYVPPAAIRERRGAQETLRRAARASLMPGSRNLLLAMLYVFLSLPLLVAFAPQ
                     SSTLSVNPSLAAWLYALAANVLHVVFLAAFTYRWVAGEPDVPEQPVKLRRTAGAARRT
                     R"
     gene            complement(9612..10910)
                     /locus_tag="VE646_10650"
     CDS             complement(9612..10910)
                     /locus_tag="VE646_10650"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZD80481.1"
                     /translation="MAQTQGTDWGQFQGEASHQGSLASGPEPPYRLAWRFRAPRGTVS
                     GPVVHGDQVVTVGTHAVIGVDLGTGRQAWSLDRSGGPIAMPAIATVKGKPAVLFTDGS
                     TKPGAALVAVALSDRTELWRAALKASSRSGIAVEGGTAYLADDDGNVYAIALSDGSVS
                     WTGTTLGEVQAPPAVADGRVFVTGRDTQNQHSELVAFDAATGRRLWVYAPSVAGSTSS
                     GPSVADGAVITGFADRSIHAFSAQDGIERWSHLALSLFSPATAPAAGPDGAYLIDVAG
                     GLYRLDPATGRRLWDYQLNDLVVRSAPVVSGNAVLVGLNDGQLAAVDRSSADLVWESR
                     PSPGLIGPIAVTGDTIVASKGGHAPGLVAFAHDDQGTLLSVPSPTRLKPVELFGTYAI
                     AAAIVLAIFLLPGRLAATRIGPPSWEAWEDVEDGHDEGAP"
     gene            complement(11133..11630)
                     /locus_tag="VE646_10655"
     CDS             complement(11133..11630)
                     /locus_tag="VE646_10655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZD80482.1"
                     /translation="MRGVRRVPTHPMLDPGPGRPGGGWGPWLQRPAAASEPWTWPVAG
                     AVIRGFDPPEDPSRSGHRGIDIATAAGTPVRAVAVGVVSFAGPVGGDLFVSIDHGGGL
                     VSTYSWLSEVLVRKGDAVAPGDVVAPPGTATPACSPRTCTWGPSSTASTWIPCRCSVR
                     PTCGG"
     gene            complement(11711..12607)
                     /gene="whiG"
                     /locus_tag="VE646_10660"
     CDS             complement(11711..12607)
                     /gene="whiG"
                     /locus_tag="VE646_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HZD80483.1"
                     /translation="MAGRIKATGEERAAKPAKGQRTPTGKRGKTTSGARARTAVATVP
                     DRQIDELGALWDEFKRGHGVEARERLILHYAPLVKYVASRVATGLPSSVEQADLVSYG
                     MFGLIDALEKFEPARGNKFETYAIPRIKGAIIDELRAMDWVPRSVRFKAREIEKAYQD
                     LETMLKRAPAESEVAERLGVSIPELHNVINQISFVSVLALDELLSVGSDRGEQVSLLD
                     TLADKNVDPTTGLEGQETRGLLAAAINSLSEREKIVVTLYYFEGLTLAEIGEILGVTE
                     SRVCQIHTKAVNQLRLQFVESE"
     gene            complement(12607..13557)
                     /locus_tag="VE646_10665"
     CDS             complement(12607..13557)
                     /locus_tag="VE646_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HZD80484.1"
                     /translation="MLEREDLPHPDRVLIERFADHLSLERHLSGHTVAAYRRDLEALV
                     VFLERQDGRLASASHEQLRRFLAHQMSLGYARSSIARRVGAVHTFYRWAAGHGVVTAD
                     PAARLGVPKVANRLPTVLRPTEAATLVEAPTSAEAPLAPEDDRPGARAVELRDRALLE
                     LLYGSGLRVGEVAGLTLDRVDLARGRVVVMGKGSKERELPLSDFARDAVADYLREGRP
                     DLGPADGPAMFFNRRGKPMTERDIRRVVGRYAERALPGRRVSPHTLRHSFATHLLEGG
                     ADIRAVQELLGHSSVATTQRYTHVSRSRLFDAYRQSHPRA"
     gene            13685..>13989
                     /locus_tag="VE646_10670"
     CDS             13685..>13989
                     /locus_tag="VE646_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZD80485.1"
                     /translation="MGGFELDLVVTEGLGDNSYVLSSGDEAVLIDPQRDVERLLRVAE
                     ARDARVRYVLETHVHNDYVSGAVQARALTGAPVLGPAKAGYAFPHRGLEEGDDVVVG"
BASE COUNT         2043 a         5222 c         4782 g         1942 t
ORIGIN      
        1 tcccggtcgg ccagcacggg gggcaggtcc gccggcacca ggtcctcgat ggccaccccg
       61 cgatcggacc gggccacctc gatctccggg agcacctccc tggtcagcga ggcgagcgag
      121 accgcgacgc gctgcagggg cagctccccc gactccagcc gcgacagatc caggagccgg
      181 tccaccagcc gtcccagccg atccgactgc gagagcatca cctgcagcgt ctccggatgg
      241 ggctcctcca ccccgtccag caggttctcc aggtgggccc ggagggccga gatgggcgtc
      301 ttgagctcgt gcgagacgtt ggccaccagg tcgcgccgca gctgctccag gctctccagc
      361 tcggcgctca tccggttgaa cgccgcggtc agctgcccca cctcgtcacg ggaagcggtg
      421 tggacccggt ggctgtagtc accggtctcc atgatccggg cggcgttcgc catgtctcgc
      481 aggggctggg tcatgccgcg ggcgagccac cgggccaggc cgagtcccac cgccgccgcc
      541 agcgctccgg ccagcaggaa ctgccaccag aagtcgaaca ggaaccggaa ggtggcggtc
      601 agccgatcca gcagcccccg cccctgcgcc gggcggaccg cgtagaccag gtggtccagc
      661 acgccctgct tcaccaccgg caccaccgcg tactggagct cgcgggtgga ccccacgtgc
      721 accagcccgt ccgtgaagcg cggcaagcgg cccggcgcgg tccccgacag cagctgcccg
      781 tccaccccca cggtcaccag cgacacgtgc tcgggcaccc gggccaggcc gcccgtggcg
      841 taccggcggg ccgccgacag cagcgccagc cggtcggcgt tctgggaggt cccccggagc
      901 gcgtacccca gcatcaggaa gatcagcacc acggtgatcc cgaccgcgat gaccagggtg
      961 gaggcgatct tccagcggat cgtgggcagg cggtccaggg gcctcatgag cccggccgat
     1021 cctggaacgc gtagccgacc ccgtgcacgg tgcggatgaa gcgcggctcg gccccgtcct
     1081 cccccagctt gcggcggatc ctggccacgt gggagtcgac cacccggccg ccggcgtagt
     1141 cgcggtagcc ccagacccga tccatcagct gctcgcgggt gaacaccacg cccgggtcgc
     1201 gggccatcgt cagcaggatc tcgaactcca gcggggtgag cgcgacctcc tggccgtcca
     1261 cgaacacccg acgccgcgag gcgtcgacgg tgagggcgcc gcgctccagg ggctccatct
     1321 cgccggaggc gcggatgcga tccacgcgcc tcaggatggc gcgcacccgc atcaccagct
     1381 cgcgcaggga gaagggcttg gtcaggtagt cgtcggcgcc cacggcgaac cccgccacct
     1441 tgtcggcctc ctccgtcctg gcggtgagca tcagcaccgg cacccagcgc tcacgctgga
     1501 tctcggtgca cacctcgagc ccgtccatcc cggggagcat gaggtccagg accaccaggt
     1561 ccggcccctc atccgccgcc gcctggatcg cctgcgggcc gtccgcggcc accacgaccc
     1621 ggtacccctc gctctcgagc cgggctcgga ccgcttcggc gatggcctcc tcgtcgtcca
     1681 cgacgagcac ggtctgctgc gccacggcag caatcgtatc ggcagctcgt ggcggtccat
     1741 cgggggccat gttgagaact tgtgtcagtg gaaccgtgcg cccggccgct cagccgaccg
     1801 gcttggcgat gtccaggtac accaccgcca cgaacaggag cacgaagagg gtggccacgg
     1861 ccgcggagat cgggatcagc ttgcgcatgt cgatcttccg gccacgggcc ttctccaccg
     1921 ccagcacggc cagatggccg ccgtcgaagg gcggcagcgg cagcaggttc aggaacccga
     1981 tgaagacgtt cacgaaggcg aagacccaca ggatgtccca gaaccgggag ccggccgccg
     2041 cctggcccac cacgcgcccc acgcccacca cgctctggac gtcggaggag gagcgggggg
     2101 cgttggtgaa cagcagccgg cccagcctgc ccagcccctc cggtccgaac agccggccca
     2161 gccccttcac cgagccgacc agcgagtccc agaccatgcc gccccctccg gtgatggagg
     2221 cgaggggacc ggcggtctcc cgggtgccgg tcagggccac gccgatccga cccacctgcc
     2281 ttccccccac ctgcgagagc accggcgtga ggtgcaccgt cagcacccgt ccgtcccgct
     2341 ccacctgcag ggcgatctcc tgaccgacgt ggcggcgggt gaaccggatc aactgcgcgt
     2401 ccgtggggtg ggagatgccg tcgatcgcca cgatctgatc gcccgcccgc aggccggcgg
     2461 tggcggcggg cgaggggtga ccggcgagcc ggggggcgac ctcggccacc agcgggctac
     2521 gggcggacgg ttggcccacc atccccagcc agatcgcgaa cagcaagaag gccagcacga
     2581 agtgggtcga tggccccgcc gcgatcacca gggcccgctg ccagatgggc ttggacccgt
     2641 aggtgcgggg cacgtcctcc gggcggacct gctcgaaggg gttcatgccc gcgatgcgga
     2701 cgtagccgcc ggccgggatc cacttcaccc cgtactcggt ctcgccccga cgggtggacc
     2761 agatccgggg cccgaacccc acgaagaact cgtccacctt gaagccgaag cgcttggcca
     2821 cgccgaagtg ggcggcctcg tgcaccagga tgacgaggat cagggcgacg aagaaggcga
     2881 tctcgccgac ggtgccggcc atcagctcct cgggatcacc gggcctcgat gatctcggcg
     2941 gcccgccgac gggcccatcc gtcggcgcgt tccaggttca ccaccgagac caccgacgcg
     3001 ggcgcctcgt gctcgtccac gaccccctgg atcacctcga cgatccgggt cagggggatc
     3061 ttgccctcca ggaacgccat caccgcgacc tcgttggcgg cgctcatcac cgcggggaac
     3121 gtgagcccga gctcgcccac ccggtaggcc aggtcgatgg ccgggaacgc ctcgcggtcc
     3181 acgggctcga aggtcagctt gcggtcggcc agcgggacgg gctccacgcc ggagggcagc
     3241 cgctcgggcc aggccagcgc gagctggatg ggcaggcgca tgtcggggac ggccagctgg
     3301 gccagcatgg tgccgtcgcg gaactcggcc agcccgtgga cgatcccctc ggggtgcacc
     3361 acgacgtgga tcttcgggta ctcgatgttg aagaggtagt gggcctcgat cacctcgagg
     3421 cccttgttca tgagggtggc ggagtcgatc gtgatcttcg gacccatctg ccagaccgga
     3481 tgggccagcg cctccttgac cgaggcccgg gccagctcgc tccgggtcca tccccggaag
     3541 gggccgccgg agccggtcac gatcacccgc ttgacctcct cgcggcgctc cccgcgcagg
     3601 agctgggcca gcgcggcgtg ctcggagtcc accgggacca ggcgctcggg ctcccccttg
     3661 atcaagtcgg tgaccagctc cccgccgatc accaggctct ccttgttggc cagggccaag
     3721 accttgccgc tctgcagcgc cgccagcgtg ggcgccagcc ccgccgcccc gaccagggcg
     3781 ttcaccacca tgtcggcctc gacgtcgcgg gccaagcgct cggcggcatc ggggccgacc
     3841 aggatctcca cgttgggaag ggtggtgccc gacgccagca tctcccgcag ctcgccggcc
     3901 gcggcctcgt ccgccatggc aacgaacggc ggcaggaact cccggatctg acccaccagc
     3961 agctcgtggt tggtcccggc cgacagcccg accaccttga accgttcggc gttgcgccgg
     4021 accacgtcca gcgcctgggt tcccaccgag cccgtcgatc cgaggatgat gagcgagcgc
     4081 atcgcctcaa gattaccccg ggaacccgac atctagcgca accataggtc accaaggccg
     4141 gaccgagccg aaccctcctc gccggcggga gccctacagc aggatcagcc ggaggaagaa
     4201 gtaggcggcc ggaaccacga acaggatcga gtcgatccgg tccagcaccc cgccgtgacc
     4261 cggcaggatg ctgcccatgt ccttcaggcc caggtctcgc ttcagcagcg actcggccag
     4321 atcgcccagc agcgcgaaga ccgccaccac cagcgccagc cccgccgcgc gtgggatcgt
     4381 gtccaggatg gcgaccgagc tggggaccac gccgatcgcg acggcgatca ccaccagcgt
     4441 cgcaccgatc gccccctcca ccgacttctt cgggctgacc gtcgacgcca gcggccggct
     4501 gccccacaag gaacccacgg cgaacgccgc cgtgtcgtag aggaaggcga ggccgatcac
     4561 ggagatcgtc accgcgcgcc cctccgggaa ccggaacagg gtcagcgcgt agcccgccag
     4621 cagcggcacg tacgccacgc ccaggaccgt gagccccagg ttgcccgccg ccccgcgccg
     4681 gtgggaggcg ggcacggtca tgtaccacag gaaggtggcg aagatcgaca gcacgaacat
     4741 cgacagcatg gccgcctcgc cccggaagta cgcgccggcc aggaccagcc cgcccgagac
     4801 cagcccgagc gccgtggccg gctggtggtg ctccttctgc agggcgcggt agagctcgcc
     4861 ctgggcccac aggacgatca cgccggccac gatcgcgaag gcccccttgc cgatcgcgat
     4921 gctgcccccc gccagcaggg cgagcgccag gccggtcagg aacgccgccg gcacgttgcg
     4981 gcccgtgcgc cgaggctcct ggtccgcccc gacctcgacc gcggtgggct cctgccagct
     5041 ggggcctccc agcccctccg cgcccacccg caccgcccgg accggaggga gctcctcctc
     5101 cgtcggctcc tcctcgtccc cgaacaggtc gggaagggtg gtggtgggca cgtcggtccc
     5161 cacgggctgg ggctcggacc cgggctccgt gatcgcctcg tcccggaccg acgccgcgaa
     5221 gtgctccgcc gccgcctcca ccgcctcggg cgtgggtgcc tcctcgccct cgtccagttc
     5281 ggcgggggtg gcgctctcga tctcctcgtc catgcccggc aggtccacgt aggcgtccgg
     5341 cggccgggtg aagctctcca ccgagatcgg ctcggggacg ggggcctcgg cgatgtccag
     5401 gtccgtcgga gccgagccct catccaagag ctcctcttct tcctcttcgg gctccgcgcc
     5461 ccggaccgct ccctggtcct cggccccggc ggtctcggct tcgccgaaga gcccttcccc
     5521 ctcggcatcc ggaccggccg ggggctcctc ctccgggatc cgaaccggct cctccggcgc
     5581 gccctgctcc tcggtggtcc gctcgcgccg gggcggggac gtctcgggcc agtccacgtc
     5641 ctggataggg gcgaagaact tgtccaggtc ctcgaacagg tcgttgtggt tctcgccctc
     5701 gggcactaga tctccttgag ctcctgctcc ttgtgcgcga tggtctcatc gacctccgcc
     5761 acgtgctgat cggtcagctt ctgcaactcc ttctccgatc gcatgaggtc atcttcagag
     5821 attgcgtggt cccgctcaag cttctccagc tcatccttgg catgacggcg gacgttgcgg
     5881 acggccaccc ggccgtcctc accccgctgg tggacgagct tgatcaggtc cttgcgacgc
     5941 tcctcggtga ggggcgggaa gctcagccgg atcaccgcgc cgtcgttcga cggcgtgatg
     6001 cccaggtcgc tggcgaggat ggccttctcc atggccgaga tggcgttctt gtcgtagggc
     6061 tggatcagca gggtcctggg ctcgggcacg gagaacgagg ccagctgttg gagcggcacc
     6121 tgcgtgccgt agtagtcgac ggtggtccgc gagaccaggg ccgaggtggc ccgtccggtc
     6181 cggatcgacg ccagctcctc gcgcagcacc gagatggcct tggtcatctt gtcccgagcg
     6241 tcccggaggg cctcgtccac gctcacgacg ccctcccgtg gaccagggtc ccgatggact
     6301 cgccggacac ggcccgacgg atgttgccct cccggccgaa gtcgaacacc acgatcggca
     6361 ggtcgttctc catgcacagg gagatggccg tggcgtccat gacctggtag ccctcgcgga
     6421 gcacgtcgat gtactcgatc gactcgaagc gcttggccgc cgggttcagg tggggatcgg
     6481 cgtcgtagac cccgtccacc ttcgtgccct tcaagatggc gtccgcgtgg atctccagcg
     6541 cccgctgggc cgcggtcgtg tcggtggaga agtagggagc gccgaggccg gcggcgaaga
     6601 tgacgatccg ccccttctcc aggtgtcgga tggcccgcag cgggatgtag ggctcggcga
     6661 tctgggccat gtggatcgac gtctgcaccc gggtggtggc gtggatcttc tccagcgcgt
     6721 cctgcagcgc cagcgcgttc atcaccgtgg ccagcatgcc catgtagtcg gcccgcgacc
     6781 gctcgatgtc gggcgcctgg cggccccgga agatgttccc gccgcccacc accacggccg
     6841 tttggacccc cagatccagc acctcgacca gctgccgggc cagcagcccg gtggcctcgg
     6901 ccgagatccc gtacccggtg tccgaggggg cgaaggcgtc gccggagagc ttgagcagga
     6961 cccgatggaa gagcggtcgg gacggtgggc gttgcggatc cgcctcggcc atgagcgctc
     7021 cctcagtccc catcctcgcc aagcttgtat cgaacgaacc ggcgcaccgc aaccttctcc
     7081 ccgaccttgg cgccgacctc gtcgaggagc tgcctgatcg tccggccgtc gtcgcggacg
     7141 aacggctgat cgagcagcac gtgatccttg tagaaggcct ccagcttgcc ctccacgatc
     7201 ttgggcacca cgtcgtcggg cttgcccatc tccctggcct gcgcctcgaa gacgtggcgc
     7261 tcctgctcca ggacgtcggg cgggacgtcc tcgcgcgaga gccaccgggg cgccgccggg
     7321 ctggccacgt gcagcgcgat gtccttggcg agcccaccga actcgtcggt gttggccacg
     7381 aagtccgtct cgcagttgac ctcgaccagc gatcccaccg tgtggttgaa gtggatgtag
     7441 gaggccaccc gaccctggtt ggccacgcgt ccggcccgct tgctcaccga ggcctgaccg
     7501 cgctcgcgga gaatctcgaa ggcccggtcc atatcgccct cggcctcctg gagggccctc
     7561 ttgcagtcca tcatccctgc cccggtccgt tcccggagct ggcggatcag gtccacgccg
     7621 atgtcgacca tcagaccccg tccttcggac cggcggaggc gtccgcggcg ggcgcggtcg
     7681 tcccgtcggg cgcctcggct gacgacggcg cggccgcctc cgggcggggg gcgggggtgc
     7741 cgggctcggg ctcgatctcg ggccccgagg ccctggtcac cctctcctcg cggccgatcc
     7801 ggcgaccctc ggcgatcgcg tcggccacca cccgggtgac gagcgccacc gagcggatcg
     7861 catcgtcgtt gccggggatc acgtagtcga cctcatccgg gtcgcagttc gtgtccacga
     7921 tggccaccac cggcacgccc agcttgcgcg cctcgttcac ggcgatgtgc tccttcttcg
     7981 tgtcgatcac gaagatggcg tccggcaagc gctccagatc ctgcatcccg cccaggttgc
     8041 gttcgagctt ctccttctcg tgacgcagct tgaggacctc cttcttcggc aggaagtcga
     8101 aggctccgcc ccgctccatc tcgcgcagct cgcgcagccg gagcaggcgc cgggacacgg
     8161 tctggaagtt cgtgagcatg ccgcccagcc atcgcgtggt gacgtggggc atgccgaccc
     8221 gggcggcctc ctcggcgatg acctcctggg cctgcttctt ggtgccgacg aatagcacga
     8281 tcccgccccg ccgggacagg tcgcgcacga aggcgtacgc ctcctcgatc ccgaccaggg
     8341 acttctcgag atcgatgatg tagatgccgg agcgttcccc gtacaggtac cggcccatct
     8401 tcgggttcca gcgcctggtc tggtgcccga agtggatacc cgcctcgagg agctcccgcc
     8461 tggtgacgac aggcacgatg cttcctcctc ccggttttgt ctcctccgtc cggatccatc
     8521 cgtccccggc ccggcgcacc gggaccccga gggcgatccc cggacgtgtg tgctcggcgc
     8581 ccgcgacgcc ggcgagcatg gagtctatca gcgggtccgg cgggccgctc ccgccgtgcg
     8641 gcgcagcttc accggctgct cggggacgtc cggctcgccc gcgacccacc ggtaggtgaa
     8701 ggccgccagg aacaccacgt gcagcacgtt ggcggccagc gcgtacagcc aggcggccag
     8761 ggaggggttc acggacagcg tgctggactg gggcgcgaag gccaccagca gcggcaggga
     8821 caggaagacg tacagcatgg ccaggagcag gttccggctc cccggcatca gcgaggcccg
     8881 cgcggcacgg cgcagcgtct cctgtgctcc ccgacgctcg cggatcgccg cgggcggcac
     8941 gtagacgaag aagaacatcg cggcgaccag gatcgccacc gatccgaaga atcccagccc
     9001 cggtcccagg aagggcagga tgatcgttcc cgtgatcatc aggctcatgg ccaacacgtt
     9061 caccagcagg acgatgggga aggcgcgcac gccgcgcagc gcaccctcca ccggaccgcc
     9121 ctcgccctcc agctcctgga cgaccagccc ggtcatcacg gacacgaaca gggcccgcac
     9181 ggccaggaac gcggcccccc aggccagccc ggcgatcccg aagccgaaga tcgcgacgcc
     9241 gttgtaggtg tcccagtacg tgccgatggg gggcagcgcg gtcatgtaga ccaggcggcc
     9301 caccgggccc tccaggccca gggtcaccat gccgagccac ccgagcagca cgaacagcag
     9361 cgtcgagccc agcaccacgg gcgaggaggc cacgatcagc agccctcggc ccaggctcct
     9421 cgagatcgag gggagcctgg actcgccggt gcctcgggcc agccatccca tgatccccgt
     9481 gggctccgaa cgccgctgct cggtccgctc cgggcgctcc cgctccttcg tggccagggt
     9541 gggcgaggac cgaccgggtc cgccgttcgt cccgcgggaa ccgggcccgg accgccggcg
     9601 ccgcttcttg ctcatggagc cccctcgtcg tggccgtcct cgacgtcttc ccaggcctcc
     9661 caggacggcg ggccgatgcg ggtggcggcc agtcggcccg gcaggaggaa gatggccagc
     9721 acgatcgccg cggcgatcgc gtaggtgccg aagagctcga ccggcttcaa ccgcgtcggc
     9781 gacggcaccg agagcagcgt tccctggtcg tcgtgggcga aggcgaccag ccccggcgcg
     9841 tggccgccct tggaggccac gatcgtgtcc ccggtgacgg cgatcgggcc gatcaggccc
     9901 ggggagggcc ggctctccca taccaggtcg gccgatgacc ggtccacggc cgccagctgc
     9961 ccgtcgttca gaccgaccag cacggcgttg ccggagacca ccggggcgct gcggaccact
    10021 aggtcgttca gctggtagtc ccacaggcgc cgaccggttg ccgggtcgag gcggtacagg
    10081 cccccggcca cgtcgatcag gtacgcgccg tcgggccccg ccgccggggc cgtggccggt
    10141 gagaacaggg acagcgccag gtggctccag cgctcgatgc cgtcctgggc cgagaaggcg
    10201 tggatggagc ggtccgcgaa gcccgtgatc accgctccat cggccacgga cggccccgag
    10261 ctggtggagc ccgcgaccga gggcgcatag acccagagcc gtcggcccgt ggccgcgtcg
    10321 aaggccacca gctccgagtg ctggttctgc gtgtcgcgac cggtgacgaa gacgcggccg
    10381 tcggccaccg cggggggagc ctggacctca cccagcgtcg tcccggtcca ggagacgctg
    10441 ccgtccgaga gggcgatcgc gtagacgttc ccgtcgtcat cggccaggta ggccgtgccc
    10501 ccctcgaccg cgatcccgct gcggctcgag gccttgagcg ccgcccgcca gagctccgtg
    10561 cggtcggaca gcgccaccgc cacgagcgcg gcgcccggct tggtgctgcc gtcggtgaac
    10621 aggaccgccg gcttgccctt cacggtggcg atcgccggca tcgcgatcgg gccgcccgag
    10681 cggtcgagcg accacgcctg gcgaccggtc cccaggtcca ccccgatgac cgcgtgcgtt
    10741 ccgaccgtca ccacctgatc gccgtggacc accggcccgg agaccgtgcc ccgaggagct
    10801 cggaagcgcc aggccaaccg gtacggtggc tccgggccgc tcgcgaggga cccctggtgg
    10861 gacgcctccc cctggaactg accccagtcg gtgccctggg tctgcgccat ggcgggggcc
    10921 gcggccaggc acagcagcgc ggccgcgagg agcgcggcac ggaggacgtg gtggagcggg
    10981 tggcgggaca ggtgagtgac ctcccgggga cggcgccgac atcctagcgc cgcctccgcg
    11041 caacgaccgt ccttcctgcg gcgatcgggt cccggcgcgg tctccgcggg acgatcctca
    11101 ccccaccgga gcgagcaggg gggcgaggaa gatcagcccc cacaggtcgg ccggaccgag
    11161 cagcgacatg ggatccacgt agacgccgtc gagcttggcc cccaggtgca ggtgcggggg
    11221 ctgcaggctg gggtggccgt ccccggaggc gccaccacgt cgccgggcgc caccgcgtct
    11281 cccttgcgca ccagcacctc cgagagccac gagtacgtgc tcaccagccc tccgccgtgg
    11341 tcgatcgaca cgaacagatc accccccacc ggcccggcga acgacacgac gcccacggcc
    11401 accgcccgca ccggcgtccc cgccgccgtg gcgatgtcga tgccgcggtg cccggatcgg
    11461 gacgggtcct ccggcgggtc gaacccgcgg atcaccgccc cggccaccgg ccaggtccag
    11521 ggctcggacg cggcggccgg ccgctgcagc caggggcccc acccgccacc aggccgccca
    11581 ggaccaggat ccagcatcgg atgcgtcggg acacggcgga cccccctcat gctcggctcg
    11641 gcccgagcct agggggcgcg gcgggatgag cagtgatcca catcaccgcc cccgccaacg
    11701 agcgcgagcc tcactccgac tcgacgaact ggagccggag ctggttcacg gccttggtgt
    11761 ggatctgaca gacccgcgac tcggtcacgc cgaggatctc gccgatctcg gccagggtca
    11821 ggccctcgaa gtagtagagg gtgaccacga tcttctcgcg ctcggacagg gagttgatgg
    11881 ccgcggccag caggccgcgc gtctcctgac cctccagccc ggtggtgggg tccacgttct
    11941 tgtcggccag cgtgtccagg agggacacct gctcgccccg gtcgctgccc acggacagca
    12001 gctcgtccag ggccaggacg gacacgaacg agatctggtt gatcacgttg tgcagctcgg
    12061 ggatcgacac gccgagccgc tcggccacct cggactcggc gggcgcccgc ttcagcatgg
    12121 tctccaggtc ctggtacgcc ttctcgatct cccgggcctt gaaccgcacg gaccgcggca
    12181 cccagtccat ggcccggagc tcgtcgatga tcgcgccctt gatccggggg atcgcgtacg
    12241 tctcgaactt gttcccccgg gcgggctcga acttctccag ggcgtcgatc agcccgaaca
    12301 tcccgtagga gacgaggtcc gcctgctcga cgctggaggg aagcccggtg gccacgcggc
    12361 tggccacgta cttcacgagc ggcgcgtaat gcaggatcag ccgttccctg gcctcgacgc
    12421 cgtgcccgcg cttgaactcg tcccacaggg cgccgagctc gtcgatctgc cgatccggga
    12481 cggtggccac ggccgtccgg gccctcgcgc cggaggtggt cttcccccgc ttgccggtcg
    12541 gggtgcgctg gcccttggcc ggcttcgcgg cccgttcctc gcccgtcgcc ttgatccgcc
    12601 ccgccatcac gccctcgggt ggctctgccg gtaggcatcg aacaggcgcg atcgggagac
    12661 gtgggtgtag cgctgggtgg tggccacgct gctgtggccc agcagttcct gcacggcgcg
    12721 gatgtcggcc cctccttcca gcaggtgcgt cgcgaacgag tgcctgagcg tgtgggggct
    12781 cacccggcgc ccaggaagcg ctcgctcagc gtatcgtccc acaacccgtc ggatgtcgcg
    12841 ttcggtcatc ggcttgcccc gacggttgaa gaacatcgcc ggcccgtcgg cggggcccag
    12901 gtcggggcgc ccctcccgca ggtagtccgc cacggcgtcc cgggcgaagt cggacagcgg
    12961 cagctcccgc tccttggacc ccttgcccat caccaccacc cggccgcgcg cgaggtccac
    13021 ccgatccagc gtgagcccgg ccacctctcc cacccggagc cccgacccgt acaggagctc
    13081 caggagcgcc cggtcgcgga gctcgacggc ccgggcgccc ggacggtcgt cctccggggc
    13141 gaggggcgcc tccgccgacg tgggggcctc caccagcgtg gccgcctccg tcggtcggag
    13201 cacggttggc agccggttgg ccaccttggg cacgccgagc ctggccgccg gatcagcggt
    13261 caccacgccg tggccggccg cccaccggta gaaggtgtgc acggctccca cccgccgcgc
    13321 gatggaggac ctcgcgtagc ccaggctcat ctggtgcgcc aggaaccggc gcagctgctc
    13381 atggctcgcc gaggccagcc gcccgtcctg gcgctccagg aacacgacga gcgcctccag
    13441 gtcccgccgg tacgcggcca cggtgtgccc ggacaggtgc cgttccagcg acaggtgatc
    13501 ggcgaagcgc tcgatcagca cccggtcggg atgggggagg tcctcccgct ctagcatcgt
    13561 tcctccggtc ggggggcggg cacgtcccgc agggtccttg taccaacggc cggagccccg
    13621 ttcaacccct cccgtcgatc ggttcgggcg cgtagcatcc ggacgggggc gtagcatccg
    13681 gacggtgggc ggcttcgagc tggacctggt ggtcaccgag gggctcggtg acaacagcta
    13741 cgtgctctcc tccggcgacg aggccgtcct gatcgatccc caacgcgacg tggagcgcct
    13801 gctccgcgtg gccgaggctc gggacgctcg ggtcaggtac gtgctggaga cccacgtcca
    13861 caacgactac gtgtccgggg cggttcaggc ccgagccctg acgggggcac ccgtgctggg
    13921 cccggccaag gccgggtacg ccttccccca ccgcgggctc gaggagggcg acgacgtggt
    13981 ggtggggga
//
LOCUS       DASQQQ010000284         5150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_402865, whole genome shotgun sequence.
ACCESSION   DASQQQ010000284 DASQQQ010000000
VERSION     DASQQQ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402865"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VE646_10675"
     CDS             complement(<1..741)
                     /locus_tag="VE646_10675"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZD80486.1"
                     /translation="MSYDRGGHRRLGALLEVGVVDLPDAVGHPVFPTTMEALVSSNGG
                     TVLDAARAALERDEAATLVVDGARLLAPLLPTSLRGRDLDEGAHRVIGPGDEIPWPQG
                     AGWLEYRPKVAAVLRRHVRGLPAQEAGGAVLGYTLVADFAVRDASGDPTPRPAGLPIA
                     IGPCIVTPDEIDPQAMFLTVRVDGEQWAKGNLNGTARDLLGSVSRSSRVEELLPGEAF
                     VSNPFDAPGVEQRIWPGAVVELEAEGIGV"
     gene            894..2459
                     /locus_tag="VE646_10680"
     CDS             894..2459
                     /locus_tag="VE646_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZD80487.1"
                     /translation="MAEQGFRITYATMSADNEELHKLYDQGIQTARSWLGQKHPFFVN
                     GEAREGSGYKEARSPIDGTVIGEFAQATREDVRDAVAAARAAFPEWANRPWQERVAII
                     RKAADLLTEQRNELASLMAMEVGKNRLEALGDVEESADLLRWNANEIEQNDGFSRPMQ
                     SMGSAGEYYDVLRPYGVWAVISPFNFPMALAAGPSSGALVAGNCVVFKPAHMGVFTGL
                     KLYEAYTAAGVPPAVFQYLSGSGSVIGDEIVNHPDVNGITFTGSYEVGMGIYKGFAKD
                     FPKPVICEMGGKNPTIVTEKADLDTATDGVLRSAFGYGGQKCSACSRVYVHRSVYDEF
                     LAVLKDKTEKIAVGDPLNKDVYLGPVIDDGAVATFEEAAAEAKKNGRVITGGERLTDG
                     EFANGTYVQPTIAEVPLDSWIWKKELFVPLVAVAPYEDLDEAIRLSNDTEYGLTAGLY
                     SEDREQIDRWLNSIEAGVIYVNRRAGATTGAWPGVQPFGGWKGSGTTGKAGGGPYYVQ
                     QYLREQSRTVIEG"
     gene            2456..3745
                     /locus_tag="VE646_10685"
     CDS             2456..3745
                     /locus_tag="VE646_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZD80488.1"
                     /translation="MSTAETTDLAQARAPHLVTEVPGPEALNVIERDGRVTSPSLPRA
                     YPFVPRRGAGSVVEDVDGNLFLDVNAGIAVTSTGHSHPKVVEAIQRQAAELIHYSASD
                     FFLPIYSEVCKRLDQITPVGGPARSFLTNSGTEAVEAALKLARYSTGRQYVIAMYGAF
                     HGRSYGSVSLTASKARYHEGFGPMLPGVLHAFYGDFEYIEKVLFKHLVVPREVAAVVV
                     EPILGEGGYLPPPEGWLAYLRELCDRHGILLVADEVQSGMGRTGRWWAMEHFDVQPDI
                     VLAGKGIASGMPLGAMIAREDLMTWEIGAHGSTYGGNPVACAAALATMDLIEGGLLDN
                     ARDVGALFLQGLREIQARHAEIVDVRGVGLMIGVRFPDHDHMVRVEEAAFHRGLLVLG
                     CGDDVIRMAPPLVFRPDQVQTALEVFEGAVAEAATGS"
     gene            3755..4138
                     /locus_tag="VE646_10690"
     CDS             3755..4138
                     /locus_tag="VE646_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZD80489.1"
                     /translation="MAAVPSSVETLTVDCAHPEELARFWAAALDYEIGEVGEEGGRIA
                     APSGHGWPVLFIVVPEGKSAKNRLHLDLRPPLSMAAEVERLKTLGATEQRVVKEGGSF
                     WTVMQDPEGNEFCVLRGPEDGWSPP"
     gene            4171..5085
                     /locus_tag="VE646_10695"
     CDS             4171..5085
                     /locus_tag="VE646_10695"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019747708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HZD80490.1"
                     /translation="MSKVTTMRDAVAELVRDGDTVAIEGFTHLICFAAGHEIIRQRKR
                     DLTLARLTPDVIYDQMIGAGVARKLVFSWLGNPGVGHLHAVRRRVEHQDPEPLELEEY
                     SHFGMVCRYVAGASNLPFFPIRSYFESDIPTANPKIATMQSPYDGDTVSVVPPLNPDV
                     TIVSAQRATANGDTQIWGLLGCQKEAAFAADRVIVVCEEVVDESVIRRDPNRTVIPGL
                     IVDAVVEQPFACHPSYAQGYYDRDNRFYLEWEQTSKDPEALQAWLKAWVYELEGHADY
                     VEKFGAEHWERLKPAEALSDRVNYGAYA"
BASE COUNT          821 a         1696 c         1875 g          758 t
ORIGIN      
        1 cacgccgatc ccttccgctt cgagctcgac caccgctccg ggccagatcc gctgctccac
       61 gccgggagcg tcgaaggggt tggagacgaa ggcctcgccc ggcaggagct cctcgacccg
      121 ggaggagcgg gacaccgatc ccaacaggtc tcgggcggtg ccgtttaggt tgcccttggc
      181 ccactgctcg ccgtcgacgc ggacggtgag gaacatggcc tgggggtcga tctcgtcggg
      241 ggtcacgatg cacggtccga tcgcgatggg caggccggcg ggtctcggcg tggggtcccc
      301 gctcgcgtcc cgcaccgcga agtcggccac cagcgtgtag cccagcacgg ctcctccggc
      361 ctcctgggcc ggcagccctc ggacgtggcg gcgcagcacc gccgccacct tcgggcggta
      421 ctcgagccag ccggctccct gcggccaggg gatctcgtcc ccggggccga tgacccgatg
      481 cgccccctcg tccaggtcgc ggccccgcag ggacgtgggc aagagcggcg cgagcagacg
      541 tgcgccgtca acgacgaggg tcgctgcctc gtcccgctcc agggcggcgc gggcggcgtc
      601 gagcaccgtg cccccgttgc tcgacacgag cgcctccatg gtggtgggga agaccgggtg
      661 cccgacggca tcgggcagat cgaccacccc cacctcgagg agcgcgccca gccgccggtg
      721 ccccccccgg tcataggaca cgagcctcaa gggaccacga tggtacgccc gcccgcgagc
      781 ggaatctcga tatccgcggg tcacttcgca acccgtgcgg acttggggag tctccgagcg
      841 cgaggctacg atagggaccg gtcatccgcg tcttcaaagg gaggcgacgt cccatggcag
      901 agcagggctt ccgcatcacc tacgcgacga tgtcggccga caacgaggag ctccacaagc
      961 tctacgacca ggggatccag accgccagat cgtggctcgg ccagaagcac ccgttcttcg
     1021 tgaacgggga ggcccgggag ggcagcgggt acaaggaggc ccgttctccg atcgacggga
     1081 ccgtgatcgg ggagttcgcg caggccaccc gggaggacgt ccgggacgcg gttgccgccg
     1141 ccagggccgc cttccccgag tgggcgaacc ggccctggca ggaacgcgtg gccatcatcc
     1201 gcaaggcggc cgacctgctc acggagcagc ggaacgagct ggcctcgctc atggccatgg
     1261 aggtggggaa gaaccgcctg gaggccctcg gcgacgtgga ggagtccgcg gatctgttgc
     1321 gctggaacgc gaacgagatc gagcagaacg atggcttctc ccggccgatg cagtcgatgg
     1381 gatcggccgg cgagtactat gacgtccttc gcccctacgg cgtgtgggcg gtgatcagcc
     1441 cgttcaactt ccccatggcc ctggcggcgg gcccctccag cggggcgttg gtggccggca
     1501 actgcgtggt cttcaaaccc gctcacatgg gcgtgttcac ggggctcaag ctgtacgagg
     1561 cctacacggc cgcgggcgtc ccgccggccg tgttccagta cctgtcgggc tccggcagcg
     1621 tgatcggtga cgagatcgtc aaccatccgg acgtgaacgg gatcaccttc accggctcgt
     1681 acgaggtggg gatggggatc tacaaggggt tcgccaagga cttccccaag cccgtgatct
     1741 gcgagatggg gggcaagaac ccgaccatcg tcaccgagaa ggcggacctg gacacggcca
     1801 ccgacggcgt gctgcgctcg gccttcgggt acggcggcca gaagtgctcg gcctgctcgc
     1861 gggtgtacgt gcaccgcagc gtctacgacg agttcctcgc ggtgctgaag gacaagaccg
     1921 agaagatcgc ggtgggcgat ccgctgaaca aggacgtcta cctggggccg gtgatcgacg
     1981 acggtgcggt ggccaccttc gaagaggcgg ccgccgaggc caagaagaac gggcgcgtga
     2041 tcacgggcgg cgagcggctg acggacggcg agttcgccaa cggcacctac gtgcagccca
     2101 cgatcgccga ggttcccctc gactcctgga tctggaagaa ggaactgttc gtgcccttgg
     2161 tggccgtggc gccctacgag gacctggacg aggcgatccg gctctccaac gacaccgagt
     2221 acgggctgac ggccggcttg tacagcgagg accgggagca gatcgaccgg tggctgaact
     2281 cgatcgaggc cggggtgatc tacgtgaacc gcagggccgg cgcgaccacc ggcgcgtggc
     2341 cgggggtgca gccgttcggt ggctggaagg gcagcggcac caccggcaag gccggtggtg
     2401 ggccgtacta cgtccagcag tacctgcgcg agcagtcgag gacggtgatc gagggatgag
     2461 caccgccgag accaccgacc tcgcgcaggc ccgggcaccc catctggtca ccgaggtgcc
     2521 cgggccggag gccctcaacg tgatcgaacg ggacgggcgg gtcaccagcc cgtccctgcc
     2581 ccgggcctac ccgttcgtcc ccaggcgggg ggccggctcc gtggtcgagg acgtggacgg
     2641 caacctgttc ctcgacgtga acgcggggat cgcggtgacc tccaccggcc actcccatcc
     2701 gaaggtggtg gaggccatcc aacggcaggc tgccgagctg atccactact cggccagcga
     2761 cttcttcctg ccgatctaca gcgaggtctg caagcggctg gaccagatca ccccggtcgg
     2821 gggcccggct cggagcttcc tcacgaactc gggtaccgaa gcggtcgagg cggccctcaa
     2881 gctggcacgc tactcgaccg ggcggcagta cgtgatcgcc atgtacggcg ccttccacgg
     2941 tcgctcgtac ggcagcgtgt cgctcaccgc atccaaggcc aggtaccacg agggcttcgg
     3001 tcccatgctg ccgggcgtcc tgcacgcgtt ctacggcgac ttcgagtaca tcgagaaggt
     3061 cttgttcaag cacctggtcg tccccaggga ggtggccgcg gtcgtcgtgg agccgatcct
     3121 cggtgagggc ggctacctgc ccccaccgga gggctggctg gcctacctcc gcgagctctg
     3181 cgaccggcac gggatcctgc tggtggccga cgaggtgcag tccgggatgg gtcggaccgg
     3241 gcgctggtgg gccatggagc acttcgatgt gcagccggac atcgtgctgg ccggcaaggg
     3301 catcgccagc gggatgccgc tgggggccat gatcgcccgc gaggacctca tgacctggga
     3361 gatcggcgct cacggctcca cctacggggg caatcccgtc gcctgcgcgg ccgccctggc
     3421 cacgatggac ctgatcgagg gtgggctgct ggacaacgct cgggacgtcg gagcgctgtt
     3481 cctgcagggc ctgcgggaga tccaggcccg ccacgccgag atcgtggacg tgcggggcgt
     3541 gggcctcatg atcggcgtcc ggttccccga ccacgaccac atggtccggg tggaggaggc
     3601 cgcgttccat cgtggcctgc tggtgcttgg gtgcggtgac gacgtgatcc gcatggcacc
     3661 gccgctggtg ttccgtccgg accaggtcca gacggcgctg gaggtcttcg agggggccgt
     3721 ggccgaggcc gcgaccggct cctgaacgag gggggtggcc gccgtgccat cgtcggtgga
     3781 gacgctcacg gtcgattgcg cgcatcccga ggagctcgcg cgcttctggg cggcggccct
     3841 cgactacgag atcggtgagg tcggcgagga gggggggcgg atcgccgctc cctcgggaca
     3901 cggttggccg gtgctgttca tcgtggttcc cgagggcaag tcggcgaaga accggctcca
     3961 tctcgacctg cgcccgccgc tctcgatggc cgccgaggtg gagcggttga agacgctggg
     4021 cgccaccgag caacgggtgg tgaaggaggg cggcagcttc tggacggtga tgcaggatcc
     4081 ggagggcaac gagttctgcg tgctccgggg gccggaggac gggtggtcgc ccccctgagg
     4141 gcgggtgggc gagaagtcgg acggtgaggt atgagcaagg tcacgacgat gcgggacgcc
     4201 gtggcggagc tggtccgcga cggtgacacg gtggcgatcg aggggttcac gcacctgatc
     4261 tgtttcgccg ccggtcacga gatcatccgt cagcgcaagc gagacctgac gctggcgcgg
     4321 ctcacccccg acgtgatcta cgaccagatg atcggcgccg gcgtggcccg caagctcgtg
     4381 ttcagctggc tggggaatcc gggcgtgggg cacctgcacg cggtccgccg tcgcgtggag
     4441 catcaggacc ccgagcccct ggagctcgag gagtacagcc acttcgggat ggtctgtcgt
     4501 tacgtcgccg gcgcctccaa ccttccgttc ttccccatcc ggagctactt cgagagcgac
     4561 atccccaccg cgaacccgaa gatcgccacg atgcagagcc cctacgacgg agacaccgtc
     4621 tcggtcgtcc cgcccttgaa ccccgacgtc acgatcgtga gcgcgcaacg cgcgacggcg
     4681 aacggcgaca cccagatctg gggcttgctg ggatgccaga aggaggccgc gttcgcggcg
     4741 gaccgcgtga tcgtggtgtg cgaggaggtc gtggacgagt cggtgatccg gcgcgacccc
     4801 aaccgcaccg tgatccccgg gctcatcgtc gatgcggtgg tcgagcagcc cttcgcttgt
     4861 caccccagct acgcccaggg gtactacgac cgggacaacc gcttctacct ggagtgggag
     4921 cagacctcca aggacccgga ggccctccag gcctggctga aggcgtgggt ctacgagctc
     4981 gagggccatg ccgactacgt ggagaagttc ggggcggagc actgggagcg cctcaagccc
     5041 gccgaggcgc tatcggaccg cgtgaactac ggggcgtacg catgagcgat gacgcagggt
     5101 ccgggggggg cctggggttc tccaggtccg agatgatgat cgtggccagc
//
LOCUS       DASQQQ010000285        11228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_79709, whole genome shotgun sequence.
ACCESSION   DASQQQ010000285 DASQQQ010000000
VERSION     DASQQQ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11228
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79709"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /locus_tag="VE646_10700"
     CDS             <1..1462
                     /locus_tag="VE646_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019869424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZD80491.1"
                     /translation="GGRSSPVVAVAGAAIDLSPRWLKDFAIRTFGTADKLALLVGIGA
                     LLAITAVLLGMASLRRPVVGAAGLLVFAGIGLAATSTRSGAVAVDAVPTVAGAVTGLA
                     ALWWLRRLAVSGATYADDAPVGRAGPEGPPVERRRLLIGALAAAGGAAFVALLGQELL
                     YRPGVSASRDAVRLPAPTDRASRVASGTDLRIAGLGRFFTPNAGFYRVDTAIFPPAVE
                     TDGWRLRIHGLVDRELSLDYRELLSRPLIERDITLTCVSNEVGGELVGNARWIGAPLR
                     DLLQEAGVRPGADQIVSTSVDGFTAGTPTAIALDGRDAILAVGMNGEPLPLAHGFPVR
                     MVVPGLYGYVSATKWLVDIELSRFGDITPYWVRQGWAPRAPIKTESRIDTPRAGASLP
                     PGRVAVAGVAWAQHRGIEAVEIRVDDEPWSEATLAAQDTVDTWRQWLYRWQATPGPHT
                     LQVRATDGTGVVQTGQRVPPFPDGATGYHTIAVTVA"
     gene            1525..2364
                     /locus_tag="VE646_10705"
     CDS             1525..2364
                     /locus_tag="VE646_10705"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HZD80492.1"
                     /translation="MTIDISQSADTRAVVGNPQTRRKVAALLLALVAGYVNAVLAIVL
                     LDISISHITGDSEDLSIALSQLHTAIVVRMGIPVATFFLGIAVGGAVMELCVRRGLKR
                     RLALMALIESLLIGGFVVSAIVVGGKHTLQAGSSLRLLWLIGLPAAAMGIQTSAVDQI
                     RGRRVRTTFLSGMVARTAQALVRCAFWVRDRMVGKTGSLRDALTSTLRIDAFDRGVFL
                     AGIWILWVGGAAMGALAELRFGALCAGFPITVLLLVGILDLIRPYEFPSPLPQEPDPR
                     ADL"
     gene            complement(2436..3254)
                     /locus_tag="VE646_10710"
     CDS             complement(2436..3254)
                     /locus_tag="VE646_10710"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HZD80493.1"
                     /translation="MPRSSFRMAGERASRGPAERGALDAEPIVHQTAPMRAGPLALVG
                     GDELNPGNEEQDAVLIRTAGDGPAFVLATAAARQHPELAVRHARDWFRALGLAVEELP
                     ARRRSDATSARNVELARAGRFFYLVGGDPGLVPDVLSETPLWDAVVEAWRAGAALAGS
                     SAGAMALGEWTLIRARYPGDRRRRPRPALTLVPGVAVIPHFDTFGAGWVEGTAATAPR
                     EDAVLVGIDERSAALWQDGVWRAFGPGGVTLIGDAGRRRFASGEHLEGFPQPLV"
     gene            3302..3673
                     /locus_tag="VE646_10715"
     CDS             3302..3673
                     /locus_tag="VE646_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZD80494.1"
                     /translation="MGQRLVHFEITADDPGRAVEFYRTVFGWEFATWEGPQTYFPTTT
                     GKDEPGIDGAVMARSELLDQAVVNTIQVQDHDASAKAVREAGGEVIQDKHAVPGVGWF
                     SYCRDTEGNVFGILEGDPSAS"
     gene            3683..4534
                     /locus_tag="VE646_10720"
     CDS             3683..4534
                     /locus_tag="VE646_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80495.1"
                     /translation="MDDVYLRRLTGRDLAFLAAAGGDRGGRASAFRTEPGRIEQLIGS
                     SEAFDALFAPWSEHPFTVASPFLVFSVLLAGTARELGATTFVREWVGPRQRIPVFDVQ
                     ALRGFLGEPLHRIFLADVLASCTRVSSGSVWVRDRRGWTRRRFSELDPVRLAALLDVV
                     PQSDRPPVYRRLGDLALFLSGVFPDAAERLFRPVQIERLERALAEVTGSGDQVPAEPP
                     GAVGLLERLGRRSYRAVLQSAPAHPFDTRKALLHVIDRFTDARRVLNVITDRRLFPVR
                     ADWFPST"
     gene            4691..5623
                     /locus_tag="VE646_10725"
     CDS             4691..5623
                     /locus_tag="VE646_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006350686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZD80496.1"
                     /translation="MILTQHLTKVYPTGVKAVDRLDLQVHEGEIFGLLGPNGAGKTTT
                     VGMLTTRVIPSAGRAFLGGVDVVAHPSTAKRLFGIVSQTNTLDRSLTVWENLYYHGRY
                     FGLGARQSRMVADQLLEVFRLADRGKADVGTLSGGMAQRLMVARGIVHRPRILFLDEP
                     TSGLDPQSRIAMWEILHEIHADGQTVLLTTHYMEEADRLCERLAIMDHGKILALDTPA
                     GLKRTVGGDTVVRMVAEGDHERLAEAMRTLPGVTEARALDGAVHLTARGSEGLLPRAV
                     AHAEAAGFVLHDLSIDVPTLETVFINLTGKDLRE"
     gene            5620..6474
                     /locus_tag="VE646_10730"
     CDS             5620..6474
                     /locus_tag="VE646_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD80497.1"
                     /translation="MKTDEREPTRQEMTLPRVEPHRSTPWIAFGSLLLRDLTVLRKNA
                     LEFVVRTIMQPFLFVFVFTYVFPKIGQAVGGSGGARTFSSVLVAGVVAIACIFQGIQA
                     VALPLVQDFGYSREIEDRVMAPLPVSAVAVEKILAGAVQGLIAAGIVFPLAAVVPATP
                     VHLNVQPVELATVLPLSALLGASLGLTIGTRVNPRQVPLLFGVVVIPITFLGATYYPW
                     ASLGPIAWLKWLVLINPLVYMSEGFRIALTPQLQHMPTWGVYLALVAFTAALALWGIQ
                     GFRNRVLS"
     gene            6844..7200
                     /locus_tag="VE646_10735"
     CDS             6844..7200
                     /locus_tag="VE646_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80498.1"
                     /translation="MWKKAILATGLVGALAVPAGVAVATTDTPTPVVARDRAAALECP
                     PDRARDRARDQARDQTQQPLRIQDPAACECDGPNGPAGFGHGGPGNGHGAMDGAGPIH
                     PAPTEGTGFRYGTGGR"
     gene            7266..7961
                     /locus_tag="VE646_10740"
     CDS             7266..7961
                     /locus_tag="VE646_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018090381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD80499.1"
                     /translation="MAPNRILVVDDEERIRSLVGSYLRKEGYQVDEVPTGEEAVAAVR
                     ARAPDLVVLDVRLPGIDGLEALRQIRRISEVYVIMLTARTEETDRVIGLEVGADDYLT
                     KPFSPRELVARIRAILRRGRRGASDGGQTLRFADLVVDPSRRGVAVDGRAVELTALEF
                     DLLAALASAPGRVFTRRQLIERVWGWDFFGEERIVDVHIRNLRKALGDVADDPRFIGT
                     VRGVGYKLIPEQP"
     gene            7958..9067
                     /locus_tag="VE646_10745"
     CDS             7958..9067
                     /locus_tag="VE646_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015747804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZD80500.1"
                     /translation="MNPWRRLRTRLFASHLLVIAVGAVAVLAVAVLLTPSLFQSRVGR
                     MGRGPGGMAGATSAELHRALTTSLDIALEVGLGTALIAAGAIAAVLGDRILRPIEAVR
                     GATHRLASGDYGQHVPIPDEEELAGLAEDVNALGLALAETEGRRARLIGEVAHELRSP
                     LTTIRASMEGLIDGVLEPTVESFAAIADEAGRLERLADDLTLLSQAEERAIPLHLEPN
                     DLGELAARAAERLRPQFDLQGVGLKIEPGPSLSVHADRDRIAQVLTNLLGNALTHTPP
                     GGTVTVRGGIHGSTAWVEVADTGAGIPPAELERIFDRFYRVPDPNHPAGRGIGLTIAR
                     GIARAHGGDVTAASDGPGTGAIFRVTIPVDRQARG"
     gene            complement(9097..9738)
                     /locus_tag="VE646_10750"
     CDS             complement(9097..9738)
                     /locus_tag="VE646_10750"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZD80501.1"
                     /translation="MVPHLDPALLAVYHALSEHTWMANVVEAVETTLEQAGLHRSVSH
                     PPAMCLLDLSGYTRLTEERGDQAAAEMAASLGTLVRSGSQAHGGHPVKSLGDGVMVHF
                     PEPAGAVLFALQMQEEIPAADLPPGHAGIAAGPLVFQDGDYFGRTVSLAARIAAFATA
                     GQVLVSDRVVEVTRLPQVTFVEIGPVELRGVAAPVRLHEAHRLDGHPGGTRGR"
     gene            complement(9913..10320)
                     /locus_tag="VE646_10755"
     CDS             complement(9913..10320)
                     /locus_tag="VE646_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80502.1"
                     /translation="MGRFTRDELARRAGVPGGFVDRAVELGILRPDHDTSFSMGDVHR
                     ARFAHGLEQGGVPLPSVASAVQSGVMSFGFFDASYWERFAGLSRTTYRELSADTGLPV
                     ELLASIGSRSATRAPVPTWCARTSWRSFRSRRR"
     gene            10397..11002
                     /locus_tag="VE646_10760"
     CDS             10397..11002
                     /locus_tag="VE646_10760"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZD80503.1"
                     /translation="MQSGAREPIIGGAAVYDRLSRIGLGSFFDHVAADIASTVPKSAR
                     VLEAGCGPGHLSIRLADRHRFQVIAVDVDAAMVRRARANARRVRGRVPSFLVGDVASL
                     AFPDRSFDLVVSTFSMHHWADPAASLVEIGRVLRPGATAIIWDLRPGVIPFHSSVPDP
                     VEHSRGSALRVARVTGRPWPWRFLLTQRIELIREDGPAGGS"
BASE COUNT         1505 a         4081 c         4009 g         1633 t
ORIGIN      
        1 gggcggtcgc tcctcgcccg tcgtggccgt ggccggcgcg gcgatcgacc tgtcgccgcg
       61 ctggctcaag gacttcgcga tccggacgtt cggcaccgcc gacaagctcg ccctgctggt
      121 ggggatcggc gcgctcctgg cgatcaccgc cgtcctgctg ggcatggcct cgctccgccg
      181 gccggtggtg ggcgcggcgg ggctcctggt gttcgcgggg atcgggctcg ccgccacctc
      241 gacccgttcg ggcgccgtcg cggtggacgc cgtccccacc gtggccgggg cggtcaccgg
      301 cctggcggcg ctgtggtggc tgcgccgcct ggccgtctct ggtgcgacgt acgcggatga
      361 cgcgccggtg ggtcgggcgg gccccgaggg tccacccgtc gagcgccgga ggctcctgat
      421 cggtgctctg gccgcggccg gaggcgccgc gttcgtcgcc ctcctcgggc aggagctgct
      481 ctaccgcccg ggggtctcgg cttcgcgcga tgccgtccgc ctcccggcgc caacggatcg
      541 ggcctcccgc gtcgcctccg gcacggacct tcgaatcgcg ggactgggcc gcttcttcac
      601 gcccaacgcg ggcttctatc gggtggacac cgccatcttc cctccggcgg tcgagaccga
      661 cggctggcgg ctccggatcc acggactggt ggaccgggag ctgagcctcg attaccgcga
      721 gctgctgtcc cggccgctga tcgagcgcga catcacgctg acctgcgtgt cgaacgaggt
      781 ggggggcgag ctcgtgggca acgcccggtg gatcggggcc ccgctccgtg acctcctcca
      841 ggaggccggg gtgcggccgg gcgccgacca gatcgtatcg acctcggtag acgggttcac
      901 cgccgggacg cccaccgcca tcgccctgga cggacgcgac gccatcctgg ccgtggggat
      961 gaacggcgag cccctcccgc tcgcgcacgg gttccccgtg cggatggtgg ttcccggcct
     1021 gtacgggtac gtctcggcca cgaagtggct ggtcgacatc gagctcagcc ggttcgggga
     1081 catcacgccc tactgggtgc gccaggggtg ggcgccccga gcgcccatca agaccgagtc
     1141 gcgcatcgac acgccgcggg ccggcgcctc cctccccccg ggacgggtcg ccgtggccgg
     1201 ggtggcctgg gcccagcacc gggggatcga ggccgtggag atccgcgtgg acgacgagcc
     1261 gtggtcggag gcgaccctgg ccgcccagga cacggtggac acctggcggc agtggctgta
     1321 ccgctggcag gccaccccgg gcccgcacac cctgcaggtc cgggccaccg acgggacggg
     1381 cgtcgtgcag accggccagc gggtcccgcc cttccccgac ggcgccaccg gctaccacac
     1441 gatcgccgtc accgtggcgt gacccaccgc gggaccaggg taagagccac ctgaacgagc
     1501 taggcgccgc cttggagcgg ttcgatgacc atcgacatca gccagtctgc cgacacacgc
     1561 gccgtggtgg gcaacccaca gacgcggcgg aaggtcgccg cgctcctgct ggcgctggtc
     1621 gccgggtacg tgaacgccgt gctggccatc gtcctcctgg acatctcgat ctctcacatc
     1681 accggggatt ccgaggacct gagcatcgcc ctctcccagc tccacacggc catcgtggtc
     1741 cggatgggga tcccggtggc gacgttcttc ctgggcatcg cggtgggcgg tgcggtgatg
     1801 gagctctgcg tccgacgggg cctgaagcgc cggctggcac tgatggcgct gatcgagtcg
     1861 ctgctgatcg gcgggttcgt ggtgtcggcc atcgtggtcg gcgggaagca cacgctccag
     1921 gccggctcat ccctccgact cctgtggctg atcgggctgc cggccgccgc catggggatc
     1981 cagacctccg ccgtggacca gatccggggg cgaagggtgc gcaccacctt cctgtccggg
     2041 atggtggccc ggaccgctca ggcgctggtc cgatgcgcct tctgggtccg tgaccggatg
     2101 gtcgggaaga cgggatcgct ccgcgacgcc ctgacatcca cgctgaggat cgatgccttc
     2161 gatcgcgggg tcttcctggc cgggatctgg atcctgtggg tgggcggggc cgcgatgggc
     2221 gcgctggcgg agctccggtt cggggccctc tgcgcggggt tcccgatcac ggtgctgctg
     2281 ctggtgggga tcctcgacct gatccgcccc tacgagttcc cctcgccgct cccgcaggag
     2341 ccggacccgc gcgccgacct ctgatcgtcg actccctccg acgcacacat cgaagggccg
     2401 ctccgagaag gggtcgcgac atgccgcccg aggtctcaca ccagcggctg cgggaacccc
     2461 tccaggtgtt cgccggaggc gaaccgtcgg cgccccgcat cgccgatcaa cgtgacgcct
     2521 cccggcccga acgcccgcca cacgccatcc tgccacagcg cggccgaacg ctcgtcgatg
     2581 ccgaccagga cggcatcctc ccgcggcgcg gtggccgccg tcccctccac ccagccggcc
     2641 ccgaaggtgt cgaagtgcgg gatcaccgcg acgcccggga ccagggtcag tgccggacgg
     2701 ggccggcggc gccggtcgcc cgggtaccga gcccggatca acgtccactc ccccagggcc
     2761 atcgccccgg ccgacgagcc ggccagcgcg gcgcccgccc gccacgcctc gaccaccgcg
     2821 tcccacaggg gcgtctcgga cagcacgtcg ggtaccaggc cggggtcccc accgaccagg
     2881 tagaagaacc ggccggcccg cgccagctcg acgttccgag ccgaggtggc atcggagcgc
     2941 cgcctggcgg gcagctcctc caccgccagc cccagagcgc ggaaccagtc ccgcgcatgg
     3001 cgcaccgcca gctccgggtg ttggcgggcg gccgccgtgg ccagcacgaa cgcgggaccg
     3061 tcgcccgcgg tccggatcag cacggcgtcc tgctcctcgt tgcccgggtt gagctcgtcg
     3121 ccacccacca gcgccagggg accggcccgc atgggcgcag tctggtggac gatgggctcg
     3181 gcgtcgagcg ctccccgctc cgccggcccg cgggaggcgc gctctcccgc catccggaac
     3241 gacgaacgcg gcatcgccct cgggcgatac ggttccagag gcggcaggag gagggaccac
     3301 catgggacag cgcctcgtgc acttcgagat cacggccgac gatccggggc gagccgtgga
     3361 gttctaccgc acggtgttcg gatgggagtt cgcgacctgg gagggaccac agacgtactt
     3421 cccgaccacc accgggaagg acgagccggg gatcgacggc gcggtgatgg cgcggtcgga
     3481 gctcctggat caagcggtgg tgaacacgat ccaggtccag gaccacgacg cctcggcgaa
     3541 ggccgtccgg gaggcgggcg gtgaggtgat ccaggacaag cacgcggtcc cgggcgtggg
     3601 atggttcagc tactgccgtg acaccgaggg caacgtgttc ggcatcctgg agggtgaccc
     3661 ctcggcgtcc tgagctatcc cgatggacga tgtgtacctg cggcgactca ccgggcgtga
     3721 cctggcgttc ctggccgccg ccggaggcga ccgagggggc cgggcctccg cgttccgcac
     3781 cgagccggga cgcatcgagc agctgatcgg cagcagcgag gccttcgacg cgctgttcgc
     3841 tccctggtcc gagcacccct tcaccgtggc cagcccgttc ctcgtgttct ccgtcctgct
     3901 ggccggcacg gcgcgcgagc tcggagccac cacgttcgtc cgcgagtggg tggggccccg
     3961 acagcggatc ccggtgttcg acgtccaggc cctgcgtggg ttcttggggg agccgctcca
     4021 ccgcatcttc ctggccgacg tgctggcctc gtgcacgcgc gtgagctcgg gcagcgtctg
     4081 ggtgcgcgac cgccgtggat ggacccgacg ccgcttcagc gagctggacc cggtccggct
     4141 ggccgcgctg ctcgacgtcg tgccccagtc cgaccggccc cccgtgtacc ggcgcctcgg
     4201 tgacctggcc ctcttcctga gcggcgtgtt cccggacgca gccgagcgcc tgttccggcc
     4261 ggtccagatc gaacggctgg agcgagctct ggccgaggtg accggctcgg gcgaccaggt
     4321 cccggccgag ccgcccggcg ccgtgggtct gctggagcgc ctgggacgac gctcctaccg
     4381 ggcggtcctg cagagcgcgc ccgcccaccc cttcgacacg aggaaggccc tgctgcacgt
     4441 gatcgatcgc ttcaccgatg cccggcgcgt cctgaacgtc atcaccgacc ggcgtctgtt
     4501 cccggtccgc gcggactggt tcccgtccac ctgacgcacc gctctggccc ccggggcctg
     4561 gaccttcggt cctggtcgga ccccctatcg tcgggcggcc tctaccatag tcggaacggc
     4621 tgccgatcgg ggcggccacg gaggtgggat ggaaccggag ccaccgggtc accgctccga
     4681 cgggcgggcg gtgatcctga cccagcacct cacgaaggtc tacccgacgg gcgtgaaggc
     4741 cgtggaccgg ctcgacctgc aggtgcacga gggcgagatc ttcgggctgc tcgggcccaa
     4801 cggggcgggc aagaccacga ccgtggggat gctgaccacc cgggtgatcc ccagcgcggg
     4861 cagggccttc ctgggcggcg tggacgtggt cgctcatccc tccaccgcca agcggctctt
     4921 cggcatcgtg tcccagacca acacgctgga tcgcagcctg accgtgtggg agaacctcta
     4981 ctaccacggt cggtacttcg ggctcggggc ccgccagtcc cggatggtgg cggaccagct
     5041 cctggaggtg ttccggctgg ccgaccgggg caaggccgat gtcggcacgc tctcgggagg
     5101 catggcccag cgcctgatgg tggcccgcgg catcgtccac cgcccccgga tcctgttcct
     5161 ggacgaaccg acctccggcc tggaccccca gtcgcgcatc gcgatgtggg agatcctcca
     5221 tgagatccac gccgacgggc agaccgtgct gctcacgacc cactacatgg aggaggccga
     5281 ccggctgtgc gagcggctgg cgatcatgga ccacggcaag atcctggccc tggacacgcc
     5341 ggccgggctg aagcggacgg tgggcgggga cacggtggtc cgtatggtcg ccgagggcga
     5401 tcacgagcgc ctggccgagg cgatgcgcac gctccccggg gtcaccgagg cgcgggccct
     5461 ggacggcgcg gtgcatctga cggcccgtgg gagcgaaggg ttgctgcccc gggccgtggc
     5521 ccacgcggag gccgccggct tcgtcctgca cgacctatcc atcgacgtgc cgacgctgga
     5581 gacggtgttc atcaacctta ccgggaagga tctgcgcgaa tgaagaccga cgagcgggag
     5641 ccgacgcgac aggagatgac cctgccccgg gtcgagcccc accggagcac gccctggatc
     5701 gccttcggct ccctgttgct gcgcgacctc accgtgctcc gcaagaacgc gctggagttc
     5761 gtggtccgca cgatcatgca gccgttcctg ttcgtgttcg tcttcaccta cgtcttcccg
     5821 aagatcgggc aggccgtcgg gggcagcggt ggggcgcgca cgttctccag cgtgctcgtg
     5881 gccggcgtgg tggccatcgc ctgcatcttc caggggatcc aggccgtcgc gctgcccctg
     5941 gtccaggact tcggctacag ccgcgagatc gaggaccgcg tcatggcccc gcttccggtc
     6001 tctgccgtgg cggtggagaa gatcctggcc ggagcggtcc agggactgat cgctgcgggc
     6061 atcgtgttcc cgctggccgc cgtggtgccg gccacaccgg tccacctgaa cgtgcagccc
     6121 gtggagctgg ccacggtgct ccccctgtcg gcgctgctgg gcgcctcgct cgggctgacg
     6181 atcggcacgc gcgtgaaccc ccgacaggtg ccgctcctgt tcggcgtggt ggtgatcccc
     6241 atcacgttcc tgggcgccac ctactacccg tgggccagcc tcggtccgat cgcctggctg
     6301 aagtggctgg tcctgatcaa tccgctcgtg tacatgagcg agggcttccg catcgcgctc
     6361 accccgcagc tccagcacat gcccacgtgg ggcgtctacc tggccctggt ggcgttcacc
     6421 gccgcgctcg ccctgtgggg gatccagggc ttccgtaacc gcgtgctctc ttgagcctcg
     6481 cgaggccggc ccctggccgc cttcctgagg cgatcgatgg ccttccgcga tcgtcaccct
     6541 ggccggaccg cgcccctcgc tacaggtgtt ggagctccac ggcatcaagg ccgaggaggc
     6601 gcgccgatcc cgggcttcga cggctcgccg gtcgtcgagg gctggtggag acggccgagg
     6661 cgctcgcccc acccggacgt cgatcctagc gctcctcggt cggcggcccc tggtcccgct
     6721 cggcccccgg acggacggcg aggcccccct tcacggaagc ttgatgatcc cgtccatcgc
     6781 cgcttgatag ccccgcggtg tcatgtcggg gacggaccga tcgagatgag aggaggtccg
     6841 atcatgtgga agaaggcgat cctcgccacg gggctggtcg gtgcgctggc cgttccggcc
     6901 ggcgtggccg tggccacgac cgacacgccg accccggtcg tcgctcggga ccgcgcggcg
     6961 gcgctcgagt gcccaccgga ccgggcgagg gaccgagctc gagaccaggc tcgggaccag
     7021 acccaacagc cgctccggat ccaggacccg gccgcgtgtg agtgcgatgg accgaacggc
     7081 cccgccggct tcggccacgg tgggcccggc aacggccacg gagcgatgga cggggccggt
     7141 ccgatacacc ccgcgccgac cgagggcacc gggttccgct acggaaccgg ggggcgttga
     7201 ggtctcccga ccgggactgg gggggcaccg cccgccggtg cctcccaggc tcttacgatg
     7261 ggcccgtggc cccgaaccgg atcctggtgg tcgatgacga ggaacggatc cgctcgctcg
     7321 tcggctcgta cctccggaag gagggctatc aggtcgacga ggtgcccacc ggcgaggagg
     7381 cggtggcagc ggtccgagct cgggcgcccg acctggtggt gctggatgtt cgcctccccg
     7441 ggatcgacgg cctggaggcc cttcgtcaga tccggcgcat ctcggaggtg tacgtgatca
     7501 tgctcacggc tcggaccgag gagaccgacc gggtgatcgg gctggaggtc ggcgccgacg
     7561 actacctcac caaaccgttc agcccccggg agctcgtggc ccggatccga gccatcctgc
     7621 gtcgcggccg gcgcggagcc tccgacggag gccagaccct ccggttcgcc gacctggtgg
     7681 tcgatccctc gcgccgcggg gtggcggtcg acggccgggc ggtcgagctc acggccctcg
     7741 agttcgacct cttggccgca ctggcctcgg cccccggccg cgtgttcacg cgccggcagc
     7801 tgatcgaacg ggtgtggggc tgggacttct ttggcgagga acgcatcgtc gacgtgcaca
     7861 tccgcaacct gcgcaaggcg ctcggcgacg tggcggacga tccccgcttc atcggcaccg
     7921 ttcgcggggt cgggtacaag ctgatcccgg agcagccgtg aacccctggc ggcgcctccg
     7981 cacgcggctg ttcgcgtccc acctgctggt gatcgcggtg ggggcggtgg cggtgctcgc
     8041 cgtcgcggtc ctcctcactc cctcgctgtt ccagtcccgt gtgggccgga tgggccgggg
     8101 accggggggg atggcaggcg ccacctcggc cgagctgcac cgggccctca cgacgtccct
     8161 ggacatcgcg ctggaggtgg gtctcgggac ggcgctgatc gcggccgggg cgatcgcggc
     8221 ggtgttggga gatcgcatcc ttcgtcccat cgaggcggtg cggggcgcga cccaccggct
     8281 ggcgagcggc gactacgggc agcacgtgcc gatcccggat gaggaggagc tggccgggct
     8341 ggccgaggac gtgaacgcgc tcggcctggc gctggccgag accgagggcc ggcgggcccg
     8401 actgatcggc gaggtggcgc acgagctccg gtcgccgctc accacgatcc gggcctccat
     8461 ggaagggctg atcgacgggg tgctggagcc caccgtggag tccttcgcgg ccatcgcgga
     8521 cgaggcgggc cgcctggagc ggctcgccga cgacctcacc ttgctctcgc aggcagagga
     8581 gcgggccatc ccgctccacc tggagccgaa cgacctcggg gagctggcgg cgcgagcggc
     8641 cgaacggctg cgcccgcagt tcgatctgca aggcgtcggc ctgaagatcg agcccgggcc
     8701 gtccctgtcc gtccacgccg accgggatcg catcgcgcag gtgctcacca acctgctcgg
     8761 gaacgccctc acccacacgc cacccggcgg gaccgtcacc gtccggggcg gtatccacgg
     8821 atcgaccgcg tgggtggagg tggcggacac cggagcgggg atcccgccgg ccgagctgga
     8881 acgcatcttc gaccgcttct accgggtccc cgatccgaac caccccgccg gacgggggat
     8941 cggcctcacg atcgcgcgag gcatcgcccg ggcgcacggt ggggacgtca ccgccgcctc
     9001 ggacggaccg ggcaccggag cgatcttccg ggtgacgatc cccgtcgatc gccaggcgag
     9061 ggggtgacgt cgctccgaga ggagaggggc gacggatcag cgaccccgag tccctcccgg
     9121 gtggccatcc agccggtggg cttcgtggag ccgcacgggc gccgcgaccc cgcgcaattc
     9181 gacgggaccg atctcgacga aggtcacctg ggggagcctg gtcacctcca cgacccgatc
     9241 gctcaccagc acctggcccg ccgtggcgaa ggccgcgatc cgagcggcca ggctgacggt
     9301 gcgtccgaag tagtccccgt cttggaagac cagcggcccg gccgcgatcc ccgcatggcc
     9361 cgggggcaga tcggcggcgg ggatctcctc ctgcatctgg agggcgaaca gcacagcccc
     9421 ggcgggttcg gggaagtgga ccatcacccc atcgcccagt gacttcaccg gatgtccccc
     9481 gtgcgcctgg gagcccgacc gcaccagcgt gccgaggctg gcggccatct ctgccgccgc
     9541 ctggtcgcca cgctcctccg tgaggcgcgt gtagccgctg aggtccagga ggcacatcgc
     9601 cggcgggtgg ctcacgctcc gatgcagccc ggcctgctcc agcgtggtct cgacggcctc
     9661 caccacgttg gccatccacg tgtgttcgga cagcgcgtga tacacagcga gcagcgccgg
     9721 atccaggtgc gggaccatcg cctcggttgc cgccgaggtt ccagcatgtc cgaccagctc
     9781 atgccggccc gcaggagcgg cccctcgatc tgggcccggt agaagctcgc atcggcctcc
     9841 gcgatccgac gcaggttctc gcccaggtcc gcacctgccg ctccatgctg aagacatccg
     9901 cacccatggc gatcaacgcc tgcgcgagcg gaatgatcgc cagctcgtcc tggcgcacca
     9961 ggtcgggacc ggggcgcgcg tagccgatcg actcccgatg ctcgcgagca gctcgacggg
    10021 caggccggta tcggcgctca gctcccgata ggtcgtacga ctgaggccgg cgaagcgttc
    10081 ccagtacgag gcatcgaaga agccgaacga catgaccccg ctctgaacgg cgctcgccac
    10141 cgatggcagc ggcacgcccc cctgctccaa gccgtgggcg aagcgggccc ggtggacgtc
    10201 ccccatcgag aaggaggtgt catggtccgg ccgcaggatc cccagttcga cggcccggtc
    10261 gacgaaccca ccaggcacgc cggcccgccg cgcgagctca tcgcgcgtga accggcccat
    10321 ggcgcagcat gctagtggat gggcagcgct cgacgcgatg ccggtcgtgc ggacgcggtc
    10381 cggtacggtt gggacgatgc agtccggagc cagggaaccg atcatcgggg gggcggccgt
    10441 ctacgacagg cttagccgga tcgggctcgg atccttcttc gaccacgtgg cggccgacat
    10501 cgcgtccacc gtcccgaaga gcgccagggt gctcgaggcg ggctgcgggc cgggccatct
    10561 ctcgatccga ttggccgatc gacaccggtt ccaggtgatc gcggtcgacg tggacgcggc
    10621 gatggtgcgg cgcgctcgag ccaacgctcg ccgggttcga ggacgcgtgc cctccttcct
    10681 ggtcggcgac gtcgcctcgt tggccttccc cgaccggtcc ttcgacctgg tggtcagcac
    10741 cttctcgatg caccactggg ccgaccccgc ggcgagcctg gtcgagatcg gccgcgtcct
    10801 ccgccccggt gccacggcga tcatctggga cctgcggccc ggggtcatcc cgttccactc
    10861 atccgtcccc gatcccgtgg agcacagccg cgggtcggcg ctgcgcgtcg cccgggtgac
    10921 cggccgacca tggccgtggc ggttcctgct cacccagcgg atcgagctga tccgcgaaga
    10981 cggaccggcc gggggctcgt agcaggtcgg cgcccgagcg ggtggtggag ccgtgccgac
    11041 gggttcggct caacctccgt ctcaggccgt tcgctctcgg cccgcgtcgc gtcccggatc
    11101 ttccccgcca tcaccgaccc cggatgctcc accatcacgt ggcggaacca tccgtccggt
    11161 cccatgatcc cgcgccagct cccgcacaca gggcacagca cggcgatctc gtccaggtcg
    11221 ggtccctc
//
LOCUS       DASQQQ010000286        10043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_267141, whole genome shotgun sequence.
ACCESSION   DASQQQ010000286 DASQQQ010000000
VERSION     DASQQQ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10043
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267141"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..658
                     /locus_tag="VE646_10765"
     CDS             245..658
                     /locus_tag="VE646_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80504.1"
                     /translation="MAWRIIAVCLLLVCVAGGASANAAPARRTYVTNDCTGVRVEPRA
                     IMFTCADGNFYARRLAWGSWHRFRAVGEGAIHRNDCEPSCAGGTFHMRRGRVVLTHRR
                     WCDDVHRYLFRRVMIRFRRPLLGDERVSWPLACPL"
     gene            739..1893
                     /locus_tag="VE646_10770"
     CDS             739..1893
                     /locus_tag="VE646_10770"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZD80505.1"
                     /translation="MLEQVPTSVTASRVHPGLLGREYSDPSVFEQERERIFFRDWFCV
                     GREQDLADPNAYLTRDIAGESILLTRDRDGALHAFFNVCRHRGSVLVEDPPAGLAKGA
                     LVCPYHSWTYALDGRLIGTPNVHDEDGLDRDAFGLRAVHVDAWDGFLFVNLSVEPTPL
                     LDQLAMDPDEPLSYGRYRAGELRVGARIVYEVAANWKIVHDNYNECLHCPGVHPELTK
                     IVPLYRKGMVWDERREDGGVFLGQGLTTFTLSGTSDKLPELPDLSEVDRCTYYGYTAF
                     PNLLVNLLSTGIMSYTLYPRSAGHTTIVSEYLFRPETVDADGFDPSEMVEFLDLVSRQ
                     DWTVCERAQRGVASRGFVQGVYPPQDELLHRFAERYRGEMARRLPSPPEA"
     gene            1923..3464
                     /gene="aceB"
                     /locus_tag="VE646_10775"
     CDS             1923..3464
                     /gene="aceB"
                     /locus_tag="VE646_10775"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HZD80506.1"
                     /translation="MGGPLEIVGPEVEDAERVLTPEALTFVAKLERGFGARRRELLQT
                     RHERQARLDAGERPDFLPPRPEVQDPGWRVAPPRADMVDRRVEITGPVERKMMINALN
                     SGARCFMADFEDANSPTWENCVQGQANLIDAIERTISLDTGEKTYRLDHETATLLIRP
                     RGWHLAEKHALLNGEPVSAALFDFGLSFFHGARRLLERGSGPSYYLPKLESHLEARLW
                     NDVFRLAQDELGIDRGSIRATVLIETILAAFEMEEILFELREHGCALNAGRWDYIFSV
                     IKKFRAHPDFLLPDRGQITMTVPFMRAYTELLVSTCHRRGAQAVGGMAAYIPSRRDPE
                     VNELALAKVREDKEREAGDGFDGTWVAHPDLVPVADEVFTKHLGDRPNQMDRLRDDVR
                     VSAPDLLDVRIPGGTVTEAGVRANVEVALRYLDSWLSGVGAAAINDLMEDTATAEIAR
                     SQVWQWVHHGAATEEGEPVTAASVRELVGSVPVADDARAVFEEVALTEPFVGFLTFSA
                     YDRLP"
     gene            3521..4879
                     /gene="aceA"
                     /locus_tag="VE646_10780"
     CDS             3521..4879
                     /gene="aceA"
                     /locus_tag="VE646_10780"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HZD80507.1"
                     /translation="MSEHTFEQAIERRVRALADGWATDPRWSGIQRTYSAEDVVRLQG
                     SVSLEHTLARLGADRLWELLQERSRVVSLGALTGGQAVQMVKAGLEAIYLSGWQVAAD
                     ANLAGQTYPDQSLYPADSVPAMVRRINNALVRADHIAWAEGDTSTRWLVPIVADAEAG
                     FGGALNAFELMKAMIEAGAAGVHFEDQLAAEKKCGHLGGKVLVPTSQFIRTLTAARLA
                     ADVADAPTVLIARTDADAAPMVTSDADPYDAPFLTGERTAEGFFTVRSGLEAAIHRSL
                     AYAPYADVLWCETSTPDLDQARDFAQAIHERFPGKLLAYNCSPSFNWRRHLEERDIQR
                     FTDALGELGYRFQFITLAGFHALNASMFELAHAYAREGMSAYVRLQQREFGMEELGYT
                     ATRHQAEVGAGYFDRIARAIGGGLSSTLGLTGSTEEAQFDGDHPSRPASRMDPDPNEA
                     RG"
     gene            4905..6632
                     /gene="aceK"
                     /locus_tag="VE646_10785"
     CDS             4905..6632
                     /gene="aceK"
                     /locus_tag="VE646_10785"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HZD80508.1"
                     /translation="MSAEPRRPTTTEVPASRLANETAPLIATAFEEHQARFAAITRRA
                     RVRFEARDWHAAVDDAVERLELYAEIVRHVEDELRRRLGPAVGDRDVWTGMKALYSGL
                     VAQREDREIAETFFNSLTRRIFATAGVDPNIEFIHSDREGPQGRPLDLLRPYPVTSTA
                     EVLERAVRDAWPDAAFADLRADARRAAERIDGHLDRPPDALEVVEAPFFRRKGAYLVG
                     RLRVGAVSVPLALALLNDGGGIAIDAVLLGENDLSILFSFTRSHFHVDVGSPADLVAF
                     LKQLMPRKPVAELYISIGFHKHGKTELYRDLLRYLGSSDEAFDLAPGTPGMVMVVFAM
                     PGYDVAFKVIRDEFPPAKVITRQGVMRKYRLVFRHDRAGRLVEAQEFEHLRFPRSRFT
                     PALLDELERGCARHVAVGTEDVVIHHAYVERRVVPLDVFIAGATSHERAAAVVDLGRS
                     IKDLAATNIFPGDLLPKNFGLTRHGRIVCYDYDELSLLTDLSFREFPPSSAEDEVADE
                     PWFGATPHDVFPEEFPNFVALPPDLREVFEARHRDLYDVGYWRRIQRRVAGGEIVDIF
                     PYEPSQRLG"
     gene            6831..7745
                     /locus_tag="VE646_10790"
     CDS             6831..7745
                     /locus_tag="VE646_10790"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HZD80509.1"
                     /translation="MAPRPRAAARTPVARPVAALWHALRARQWLKNVLVVAAPGAAGI
                     LARPHVLAEIGIAFTALCLVASATYLVNDVLDAPADRSHPTKRLRPVASGALHPAVAL
                     AAAGVLFGAGLALGTALGERFLLVVASYVLITIAYSTWLKRVEVFDLAAVASCYVLRA
                     LAGAAAASVAISPWFLILTSSAAAFVVAGKRSADRSALAGSGEVRLNGNGSDYPMAYL
                     RYVRTLASGVAVAAYCLWAFDIPHLVDGIAWSQISIVPFALAILRYALLIERGRGGQP
                     EDVVLHDRPLQLIGAVWLVVYAVGIYTR"
     gene            7742..9130
                     /locus_tag="VE646_10795"
     CDS             7742..9130
                     /locus_tag="VE646_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016824916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZD80510.1"
                     /translation="MRTARTGEGRLIAGWGRTAPTRARIEEVHRPQDVARALAAAGPR
                     GIVARGLGRSYGDVAQNAGGHVLHMRGLSGIRQLDTGRGLVRVAAGTSLDRLLRELVP
                     LGWLPPVLPGTRFVTIGGALANDVHGKNHHRDGGFADHVEDLTIVTADGHPRRITPST
                     DPDAFAATAGGLGLTGVVTEVALRLLPITTAHMVVDTDRTADLDELMARMQQEDHRYR
                     YSVAWVDCLARVRQLGRGVLTRAEHALSDQLGPRRSRRGRAFRARTLGAVPPGVPRLV
                     GRATAAAFNEAWFRKAPRRERGRVRSLASFFFPLDAVGEWNRLYGPRGLIQYQLAVPD
                     GQERAVVAAIERLSRAGCPSSLAVLKRFGPGRGMLSFPIAGWTLALDLPAAHGGLGRV
                     LDGLDELVVEAGGRVYLAKDARLRPELLREMYPELEAWREVRAAMDPDRMFRSDLERR
                     LMVAGDERKETW"
     gene            9127..9888
                     /locus_tag="VE646_10800"
     CDS             9127..9888
                     /locus_tag="VE646_10800"
                     /EC_number="1.1.1.333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenylphospho-beta-D-erythro-pentofuranosid-
                     2-ulose 2-reductase"
                     /protein_id="HZD80511.1"
                     /translation="MKDGSGSVQTALILGGASDIGLATARRLVRDRTRTVVLAARKPE
                     RLAEEADRLRTLGASRVELLPFEALELESHERVIDDAFSLTGDLDVAVLSFGLLGDQR
                     VAERDAAAAVEIVRTNQLASTSLLIPLAERMRGQGHGTIAVLSSVAGERGRRSNFVYG
                     SSKAGLDVFCQGLGDALHGSGVHLLVLRPGFVRSKMTQGIPPAPLSVDPEDVAAALVR
                     GVRSGAEVVWVPWMLRWVMSGLRHLPRPIFRRLEI"
BASE COUNT         1365 a         3532 c         3657 g         1489 t
ORIGIN      
        1 gagcctgcca cccaccgcag ccgcggctga agggccgttc gacccgggtc ggtcggtctg
       61 cgaggacgcc gcttccctgg tcgctggatg ccggttgtag cgggcggcgg gtcccgtccg
      121 cgccgtccct ccagagcacg gtcccccgcc ttgcgggtcc ccagcctctg tcgcatcggg
      181 cggccccctg gcatcgtcgc acccatcgat ccgcaccact gcagacctcc gacggagggc
      241 ggtcgtggcc tggaggatca tcgcggtctg cctgctcctc gtgtgcgtcg cgggaggggc
      301 ttcggcgaac gcagcccccg ctcgtaggac ctacgtgacc aacgactgca ccggcgtgcg
      361 cgtcgagcct cgggcgatca tgttcacctg cgcggacggc aacttctacg ccaggcggct
      421 ggcgtggggg agctggcatc gcttccgcgc ggtgggtgag ggggcgatcc atcgcaacga
      481 ctgcgagccc tcctgcgccg ggggaacctt ccacatgcgg aggggccggg tggtgctgac
      541 ccaccggcgt tggtgcgacg acgtccatcg ctacctgttc cgcagggtga tgatccggtt
      601 ccggcgaccg ctgctggggg acgagcgcgt gtcctggccg ctcgcctgtc ccctgtaacc
      661 accggaccct gcgcttgaca gtcccccgct ggcgaggctg aatgctcgtt caaccacccc
      721 cggcaggagg ccagcgccat gctcgagcag gtcccgacgt ccgtcaccgc atcccgcgtc
      781 caccccgggc tcctgggccg cgagtacagc gacccgtcgg tcttcgagca ggagagggaa
      841 cggatcttct tccgggactg gttctgcgtg gggcgcgagc aggacctcgc cgatcccaac
      901 gcctacctca cgcgcgatat cgccggggag tcgatcctgc tcaccaggga tcgggacggc
      961 gccctccatg ccttcttcaa cgtgtgccgg catcgaggaa gcgtgctcgt cgaggatccc
     1021 ccggccggcc tcgccaaagg ggcgctcgtc tgcccctacc acagctggac ctacgccctc
     1081 gacggccggc tcatcggcac cccgaacgtg cacgacgagg acgggctgga ccgggacgcg
     1141 ttcggcctgc gagccgtgca cgtggacgcc tgggacggct tcctgttcgt gaacctctcc
     1201 gtcgagccga ccccgctgct cgaccagctg gcgatggacc ccgacgagcc cctgtcctac
     1261 gggcggtacc gggccggcga gctgcgggtg ggcgcccgga tcgtgtacga ggtcgcggcc
     1321 aactggaaga tcgtgcacga caactacaac gagtgcctgc actgtccggg cgtgcacccc
     1381 gagctcacga agatcgtgcc gctgtaccgc aagggcatgg tctgggacga gcggcgcgag
     1441 gacggcggcg tgttcctggg ccagggcctc accaccttca ccctgagcgg cacctccgac
     1501 aagctccccg agctccccga cctgtccgag gtcgatcggt gcacctacta cgggtacacg
     1561 gcgttcccca acctgctggt gaacctgctg tccacgggga tcatgagcta cacgctgtac
     1621 ccgcgctcgg ccgggcacac cacgatcgtg tccgagtacc tgttccggcc cgagaccgtg
     1681 gacgccgacg ggttcgaccc ctcggagatg gtggagttcc tggacctggt cagccggcag
     1741 gactggaccg tgtgcgagcg ggcccaacgc ggcgtggcct cgcgcgggtt cgtgcagggc
     1801 gtctacccac cgcaggacga gctcctccac cgcttcgcgg agcgctaccg aggcgagatg
     1861 gcccgccgcc tgccctcgcc accggaagcg taacggcggg cgggtcgggg aagatgctgc
     1921 agatgggggg acccctggag atcgtggggc cggaggtcga ggacgccgag cgcgtgctca
     1981 cgcccgaggc cctgaccttc gttgcgaagc tcgagcgcgg gttcggggct cggcgccgcg
     2041 agctgctgca gacccgccac gaacggcagg cccggctgga cgcgggcgag aggcccgact
     2101 tcctcccccc gcgccccgag gtgcaggacc ccggctggcg ggtggccccg ccccgtgcgg
     2161 acatggtcga ccgccgggtc gagatcaccg gcccggtcga gcggaagatg atgatcaacg
     2221 ccttgaactc gggcgcgcgg tgcttcatgg ccgacttcga ggacgccaac tcccccacct
     2281 gggaaaactg cgtgcagggc caggccaacc tgatcgacgc gatcgagcgg accatctcgc
     2341 tcgacaccgg cgagaagacc taccggctgg accacgagac cgccacgctc ctgatccggc
     2401 cccgcgggtg gcacctggcc gagaagcacg cgctgctgaa cggcgagccg gtctcggctg
     2461 cgctgttcga cttcgggctg tccttcttcc acggagcgcg ccgcctgctc gaacgcggga
     2521 gcggcccttc ctattacctg cccaagctcg agagccacct ggaggcgcgc ctgtggaacg
     2581 acgtgttccg cctggcccag gacgagctcg gcatcgatcg cggttcgatc cgcgccaccg
     2641 tgctgatcga gacgatcctg gccgccttcg agatggagga gatcctgttc gagctgcgcg
     2701 agcacggctg cgcgctgaac gccggacgat gggactacat cttcagcgtg atcaagaagt
     2761 tccgcgccca tccggacttc ctgctccccg accgggggca gatcacgatg acggtcccgt
     2821 tcatgcgcgc ctacaccgag ctgctggtct ccacctgcca ccgacggggc gcccaggcgg
     2881 tgggcggcat ggccgcctac atcccctcgc ggcgcgatcc cgaggtgaac gagctcgcgc
     2941 tggccaaggt gcgggaggac aaggagcgcg aggccggcga cgggttcgac gggacctggg
     3001 tggcgcaccc ggacctggtg ccggtggccg atgaggtgtt caccaagcac ctcggcgacc
     3061 ggccgaacca gatggaccgc ctgcgcgacg acgtccgggt gagcgcgccg gacctgctcg
     3121 acgtccggat ccccggcggc accgtgaccg aagccggggt gcgggccaac gtggaggtcg
     3181 cgctgcggta cctggactcg tggctctcgg gcgtgggagc cgcggcgatc aacgacctga
     3241 tggaggacac ggccaccgcc gagatcgccc gctcccaggt ctggcagtgg gtgcaccacg
     3301 gcgccgccac cgaggagggc gagccggtga cggccgcctc ggtgcgcgaa ctggtcggat
     3361 ccgtcccggt ggcggacgac gccagggccg tgttcgagga ggtcgctctc acggagccgt
     3421 tcgtggggtt cctgaccttc tccgcctacg accggttgcc ctgacctcgg gttccccctg
     3481 cgctcgccgg gtatggcacg gtattgggga gggaggggtc atgagcgagc acacgttcga
     3541 gcaggcgatc gagcggcggg tgcgggccct ggcggatggg tgggcgacgg atccccggtg
     3601 gtcggggatc cagcgcacct attcggccga ggacgtcgtg cggctgcagg gctccgtgtc
     3661 cctggagcac acgctggccc ggttgggcgc cgatcggctg tgggagctgc tgcaggagcg
     3721 ctcgcgcgtg gtgtccctcg gggcgctcac cggtggtcag gccgtgcaga tggtcaaggc
     3781 cggactggag gcgatctacc tctcgggctg gcaggtggcg gccgacgcca acctggccgg
     3841 gcagacctac cccgatcaga gcctgtaccc ggccgattcc gtcccggcca tggtgcggcg
     3901 gatcaacaac gcgctggtgc gggccgacca catcgcctgg gccgaggggg acacctcgac
     3961 ccgctggctg gtgccgatcg tggccgatgc cgaggcaggg ttcggtgggg cgctcaacgc
     4021 gttcgagctg atgaaggcca tgatcgaggc cggtgcggcc ggggtgcact tcgaggatca
     4081 gctggccgcc gagaagaagt gcgggcacct ggggggcaag gtgctggtgc ccacctcaca
     4141 gttcatccgc acgctgacgg cggcgcgcct ggcggcggac gtggcggacg cgcccaccgt
     4201 cctgatcgcc cggaccgacg ccgacgctgc gcccatggtg accagcgacg ccgaccccta
     4261 cgacgctccg ttcctgaccg gcgagcgcac cgcggagggg ttcttcaccg tgcgttcggg
     4321 gctcgaggcc gcgatccacc ggtccctggc gtacgcgccg tacgcggacg tgctgtggtg
     4381 cgagacctcg accccggacc tggatcaggc cagggacttc gcccaggcga tccacgagcg
     4441 cttcccgggc aagctgctgg cctacaactg ctcgccctcg ttcaactggc gtcgccacct
     4501 ggaggagcgg gacatccagc ggttcaccga tgcgctgggc gagctggggt accggttcca
     4561 gttcatcacc ctggccggct tccacgccct gaacgcctcg atgttcgaac tggcccacgc
     4621 atacgcgcgg gagggcatga gcgcctacgt ccgcctccag cagcgcgagt tcgggatgga
     4681 ggagctgggc tacacggcca cccgccacca ggccgaggtc ggcgcgggct acttcgaccg
     4741 gatcgcccgc gcgatcggtg gcgggctcag ctcgacgctc gggctgaccg gctccaccga
     4801 ggaggcgcag ttcgacggcg atcacccctc gcgcccagcc tcgcggatgg accccgatcc
     4861 gaacgaggcg cgagggtgag cccaccggtg agccggtagc gggcgtgagc gccgagccca
     4921 ggcggcccac gaccacggag gtgccggcca gccggctggc gaacgagacg gctccgctga
     4981 tcgcgacggc cttcgaggag caccaggccc ggttcgccgc gatcacgcga cgggcccggg
     5041 tccggttcga ggcacgcgac tggcacgcgg ccgtggacga tgccgtggag cggctggagc
     5101 tgtacgcgga gatcgtgcga cacgtcgagg acgagctccg ccggcggctg ggtccggcgg
     5161 tgggcgaccg cgatgtctgg accggtatga aggccctcta ctcgggtctg gtggcccagc
     5221 gggaagaccg cgagatcgcc gagaccttct tcaactcgct cacccgacgg atcttcgcca
     5281 ccgcgggcgt ggaccccaac atcgagttca tccacagcga tcgcgagggt ccacagggac
     5341 gcccgctcga cctgctccgt ccttacccgg tgacctccac cgcggaggtc ctggagcggg
     5401 cggtccgcga cgcgtggccg gacgcagcct tcgcggatct gcgcgccgac gcccgccgag
     5461 cggccgagcg gatcgacggt cacctcgacc gaccgcccga cgcgctggaa gtggtggagg
     5521 ccccgttctt ccgccgtaag ggcgcctacc tggtgggacg gctgcgcgtg ggggccgtct
     5581 cggtccccct cgcgctggcc ctcctgaacg acggcggggg gatcgcgatc gacgcggtcc
     5641 tgctcgggga gaacgacctg tcgatcctgt tcagcttcac ccgctcgcac ttccacgtgg
     5701 acgtgggatc gccggccgac ctggtggcct tcctcaagca gctgatgccc cgcaagcccg
     5761 tggccgagct ctacatctcg atcgggttcc acaagcacgg caagaccgag ctgtaccggg
     5821 acctgctgcg gtacctgggg agcagcgacg aggccttcga cctggcgccc gggaccccgg
     5881 gcatggtgat ggtggtgttc gcgatgcccg gctacgacgt ggccttcaag gtgatccgcg
     5941 acgagttccc gcccgcgaag gtgatcaccc gccagggcgt gatgcgcaag taccggctgg
     6001 tcttccggca cgatcgggcc ggaaggctgg tggaggccca ggagttcgag cacctgcgct
     6061 tcccgcgatc gcggttcacc ccggccctgc tggacgagct cgagcgcggg tgcgcgcgtc
     6121 acgtcgcggt gggaacggag gacgtggtga tccaccacgc gtacgtggag cgccgggtgg
     6181 tgccgctgga cgtgttcatc gccggggcca cctcgcacga gcgggccgcc gccgtggtcg
     6241 acctcggccg gtccatcaag gacctggcgg ccacgaacat cttccccggg gacctgctgc
     6301 ccaagaactt cggcctgacc cgacacggcc ggatcgtgtg ctacgactac gacgagctgt
     6361 cgctgctgac cgacctgtcg ttccgagagt tcccgccgtc cagcgccgag gacgaggtgg
     6421 ccgacgagcc ctggttcggg gccacccccc acgacgtgtt ccccgaagag ttccccaact
     6481 tcgtggcgct gccgccggac ctgcgggagg tgttcgaggc ccgccaccgg gatctgtacg
     6541 acgtgggcta ctggcggagg atccagcgcc gggtggccgg cggcgagatc gtcgacatct
     6601 tcccctacga gccctcccaa cgcctggggt gacgaggccc ggtcctgggg acgggtcttc
     6661 gagcctgcgc ggagggccag ccgacccggg ttaggagttg gttaggaacc cggtggaaac
     6721 acaagctccg cgggtatcgc cgggcgaagg tggtacgagg gtgagctccg tcgccctcca
     6781 caggacccgg gaggcacgtg caggaagcga ctcgatcggc gccggcgagc atggcgccca
     6841 ggccccgagc ggccgcccgg acccccgtcg cgcgacccgt ggcggcgctg tggcacgccc
     6901 tgcgggcgcg ccagtggctg aagaacgtcc tggtggtggc cgcgccgggt gcggcgggga
     6961 tcctggcccg tccccacgtc ctggccgaga tcgggatcgc cttcaccgcc ctctgcctgg
     7021 tggccagcgc cacctacctg gtgaacgacg tgctggatgc tcccgccgac cgctcgcacc
     7081 ccaccaagcg cctgcgtccg gtggcctccg gagcgctcca ccccgcggtc gcgttggcgg
     7141 cggccggcgt gctgttcggg gccggcctgg cactggggac cgcgctcggg gagcgcttcc
     7201 tgctggtggt ggcctcctac gtgctgatca cgatcgccta cagcacgtgg ctgaagcggg
     7261 tggaggtgtt cgacctggcc gcggtcgcct cctgctacgt cctgcgcgcc ctggccggag
     7321 cggcggcggc ctcggtggcc atctccccat ggttcctgat cctcacctcc tcggcggcgg
     7381 cgttcgtggt ggccggcaag cgctccgccg accggtcggc cctggccggc agcggtgagg
     7441 tccggctgaa cgggaacggc tccgactacc cgatggcgta cctgcgctac gtgcggacgc
     7501 tcgcctccgg cgtggcggtg gctgcgtact gcctgtgggc gttcgacatc ccccacctgg
     7561 tggacggcat cgcttggagc cagatctcga tcgtcccctt cgccctggcg atcctgcggt
     7621 acgcgctgct gatcgaacgg ggtcgtggcg gccagcccga ggacgtggtc ctgcacgacc
     7681 ggccgctgca gctgatcggg gcggtgtggc tggtggtgta cgcggtgggg atctacacgc
     7741 gatgaggacc gctcgcaccg gggaggggcg actgatcgcc ggctggggcc ggaccgctcc
     7801 cacgcgagcc cggatcgagg aggtgcaccg accccaggac gtggctcggg ccctggccgc
     7861 cgccggcccc cgaggcatcg tggcccgcgg gctgggacgc agctacggcg acgtcgccca
     7921 gaacgccggg ggtcacgtcc tgcacatgcg ggggctgtcc gggatccgcc agctcgatac
     7981 gggccgcggg ctggtccggg tggccgccgg caccagcctg gaccgactgc tgcgcgagct
     8041 cgtccccctg ggatggctgc ctccggtgct ccccggaacc cggttcgtga cgatcggcgg
     8101 ggcgctcgcg aacgacgtcc acggcaagaa ccaccaccgc gacggcggct tcgccgacca
     8161 cgtggaggac ctcacgatcg tgaccgccga cggccatccc cggcggatca cccccagtac
     8221 ggacccggat gcgttcgccg ccacggcagg agggctcggc ctcaccggcg tggtgaccga
     8281 ggtcgccctc cgcctgctgc ccatcaccac cgcccacatg gtcgtggaca ccgaccgcac
     8341 cgccgacctg gacgagctca tggcccggat gcagcaggag gatcatcggt accggtactc
     8401 ggtggcctgg gtggactgcc tggcgcgcgt acggcagctc ggccggggcg tcctcacccg
     8461 cgccgagcac gcgctctccg atcagctggg cccccgccga tcgcgccggg gtcgagcctt
     8521 ccgggcccgg acactcggcg cggtgccccc tggggtcccc cggctggtgg gacgggccac
     8581 ggccgcggcg ttcaacgagg cgtggttccg caaggcgccg cgccgggagc gaggacgggt
     8641 ccgatccctg gcctccttct tcttccccct ggacgcggtg ggggagtgga accgcctcta
     8701 cgggccgcga gggctgatcc agtaccagct cgcggttccg gacgggcagg agcgcgccgt
     8761 ggtcgccgcg atcgaacggc tctcgagggc gggctgcccg tcgtcgctgg ccgtgctgaa
     8821 gcggttcggg ccgggccggg ggatgctctc gttccccatc gccgggtgga ccctggccct
     8881 ggatctcccg gccgcccatg gcgggctggg gcgggtgctg gacgggctcg acgagctggt
     8941 ggtggaggcc ggggggcggg tctacctggc caaggacgct cgcctgcgtc cggagctgtt
     9001 gcgcgagatg tacccggagc tcgaggcctg gcgggaggtg cgggcggcca tggatcccga
     9061 ccggatgttc cgctctgacc tggagcggcg cctgatggtg gcgggcgatg agcggaagga
     9121 gacctggtga aggacggatc gggttcggtg cagacggcgc tgatcctggg cggagcctcg
     9181 gatatcggcc tggccaccgc acggaggctg gtccgcgatc gcacccgaac ggtggtcctc
     9241 gcggcccgca agcccgagcg gctggccgag gaggccgacc gcctcaggac cctgggggcg
     9301 agccgcgtgg agctgctccc cttcgaggcc ctggagctcg agagccacga acgggtgatc
     9361 gatgacgcgt tctcgctgac cggggacctg gacgtggcgg tgttgtcgtt cggcctcctg
     9421 ggcgatcagc gggtggccga gagggacgcc gccgcggccg tggagatcgt gcggacgaac
     9481 cagctggcgt cgacctcgtt gctgatcccg ctggccgagc ggatgcgggg ccagggtcac
     9541 gggaccatcg cggtgctgtc ctcggtggcc ggcgagcggg gccggcggtc caacttcgtg
     9601 tacggctcgt cgaaggccgg cctggacgtg ttctgccagg ggctgggcga cgcactgcac
     9661 ggctcgggcg tgcacctgct ggtgctccgc ccggggttcg tgcgaagcaa gatgacccag
     9721 gggatccctc ccgcccccct ctcggtggac cccgaggacg tggccgcggc cctggtgcgc
     9781 ggggtccgct cgggcgccga ggtggtgtgg gtgccctgga tgctccgatg ggtgatgagc
     9841 gggctgcggc acctgccccg accgatcttc cggcggctgg agatctgatg gagggatccc
     9901 acccggcctc gctgccctcc ctcaccgggc gccgccctcc ggcccggcgc cggcaccggt
     9961 tccgtcggat cgcagccctg atcctggtcg tcctggcgat ccccaccggc tggtccttct
    10021 cccgtgccct gacggcgccc ggc
//
LOCUS       DASQQQ010000287         6176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_348959, whole genome shotgun sequence.
ACCESSION   DASQQQ010000287 DASQQQ010000000
VERSION     DASQQQ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348959"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1554)
                     /locus_tag="VE646_10805"
     CDS             complement(<1..1554)
                     /locus_tag="VE646_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HZD80512.1"
                     /translation="MAEDPLVRVADRPEAPRPGLVTRAKTRLFSDGSLTKKASLNSVA
                     QALDYAARAITGLVVNPILLHHLGDTGFGNWQVLQRLIGHTNPAGGRPAEALKWFVAN
                     RQSSTDLEDKRRAVGSAVAVWLIFLPILLPIGLAISWFAPGWMHVPPAGAWIIRIAGM
                     VLIADIIVAGITNIPWAVMAGQNVAYKRMGLTASLEFVEGILLVVAAVMGFGLVGIAV
                     ATVAGTTVIGATYFWLTRIYVPWFGYAKPDMPAVRRFLSLSWWFLVWNFVMKVTMGGD
                     IIVLGIAGTASEVTVYSLTRFVPITIMAGVTSLVFGMAPGLGGLIGAGETSRASRVRN
                     ETMAAAWMMATVAGAGVLLWERSFLRLWVGERYYPGLGPTLLIVLMVVQLTVIRVDSN
                     IIDLTLNLRRKVLLGVVSIGLSVALAWLFVAEYHLGIAGVVTGFVLGRIPQSLAYPSM
                     IGRILHIPLWSQLKGLLRGALTTGALFGLTAVGGEILTVRTWIGLIVVSVVSGAVLIA
                     GAFFGGLSAQ"
     gene            complement(1557..3017)
                     /locus_tag="VE646_10810"
     CDS             complement(1557..3017)
                     /locus_tag="VE646_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80513.1"
                     /translation="MRRLCRRLSEDGVAYCHWKSNAALDRSALAQSDLDLLVDPDHGE
                     RFARIVHDLGFKRGRPPQARQRMPGVYHAYGFDEPSGRVVHLHVHHQLVLGDDMTKNY
                     RLPVERAYLVSAEQGPLFRVPAPAFELVVFVLRMVLKHSTPDAMLTGQGSLSKGEAHE
                     LGYLRALATSEEGRGLVREHLPSVGPELWDRCLRCLEPGTSPLVRARTAARLERALAP
                     QARRSPALDPFLRVWRRGRRALDRRILHRRPPRSRLEGGGALIAIVGGDGAGKTTAVE
                     EVHRWLSRDLDAIAMHLGKPPRSLTSALVHAVWRGAGGGPQGTPSPEGRTPVELTDDD
                     PMTTRRLLKLVRKVLTSRDRYLATVRARRLASQGAIVICDRFPLAEVTQMDGPATARL
                     SRSRRRHPLVAILARMEERYYRRAGYPDVLIVLRVDPDVAVARRRGVESEGFVRRRAE
                     EIWALDWSRTPALVIDAGRPRDEVLARIRAAIWGGL"
     gene            complement(3373..4368)
                     /locus_tag="VE646_10815"
     CDS             complement(3373..4368)
                     /locus_tag="VE646_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZD80514.1"
                     /translation="MIRKAVARTVKAGSNLHRPDGRPNVFLYSTPRSGSTWLMELIWS
                     QPGFKTCNQPLYLENPVVRRRSGIRDWDELYGTEVTQKLQRYLGRICAGRIGFTNPSP
                     LRTPYRPISHRIVFKEIHAPADRVNWIRDTFDARVVYLLRHPIAVAISSERWPVLPTF
                     LHGDYRRHFRPEQLTEAERIAAQGTDLERGVLSWCLQNAVALREATPDWAVVTYEQLV
                     LDPEPVIQELCAKLALPSPERMRDRLGVPSVNVRFKSAEETRRLLTEGAAGDRPILVE
                     KWRRKVDRGAESRVMRILELFELDAYRDDDVLPDARLWIPGRPAEAEPVERAGWG"
     gene            complement(4365..5126)
                     /locus_tag="VE646_10820"
     CDS             complement(4365..5126)
                     /locus_tag="VE646_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80515.1"
                     /translation="MDLARTRPAPPADVTAADGAGAHGPEVRHRPVLVELMGPATAGK
                     TSLARALRSREGRVAAGVRVPRRIWFPRLVLRLAGLVPIWLRARPRGRWFTWNEMKSI
                     AFLDSWLRAVQRGTWSASPLVVLDHGPIYRLARLREFGPPLARSERFGRWWRTMLDGW
                     AHALDLIVSLDAPDEVLLGRVERRGHWYLSSEPSVEQQRAFLERYRRAFAEVLEMAGD
                     AAPALLPVRSDQRAPEQIAQQVLAVTGRIEGRSAR"
     gene            complement(5230..6078)
                     /locus_tag="VE646_10825"
     CDS             complement(5230..6078)
                     /locus_tag="VE646_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80516.1"
                     /translation="MPTRRPGQSSALTASRHVLRRWISLRPGVYLPLARWKNDHTALG
                     EQTELLVDGFTRSAGTFAAIAFQFAQNDHVRVAHHMHAAAAMVMAARRGVPALLTVRE
                     PEPTILSAAVREPGIPLTQWLKTYVAFYERIRPFRSGFVVGAFEEVTTDLGAVIRRVN
                     ERFGRAFRSFEHTEQNVEAVFALIDERAEGPPWQPHLNRFVSGLISLDQYRAATERYR
                     LEGRRPAIPELRVQRPTAERRAAKEELQAAYRGPSLAPLRAAAERAYAGLASPAPGDR
                     RSEGRG"
BASE COUNT          933 a         2286 c         2184 g          773 t
ORIGIN      
        1 ctgggcggac agcccgccga agaacgcccc ggcgatcagc acggcgccgg acaccaccga
       61 gaccacgatc agccctatcc acgtgcgcac cgtgaggatc tccccgccca ccgcggtgag
      121 gccgaacagg gcgcccgtgg tgagcgcccc acgcagcaga cccttcagct gcgaccagag
      181 cgggatgtgc aggatccgac cgatcatcga cgggtaggcc aggctctgcg ggatccgtcc
      241 cagcacgaac cccgtcacca ccccggcgat ccccaggtgg tactcggcca cgaagagcca
      301 ggccagggcc acggacaggc cgatcgacac gacccccagc agcaccttgc gccgcaggtt
      361 cagggtgagg tcgatgatgt tcgagtcgac ccggatcacc gtgagctgca ccaccatgag
      421 cacgatcagc agcgtgggcc ccaggcccgg gtagtagcgc tcgccgaccc acagccggag
      481 gaaggatcgc tcccacagca gcacgccggc gccggccacg gtggccatca tccacgcggc
      541 cgccatggtc tcgttgcgca cgcgcgaggc ccgggaggtc tcccccgccc cgatcagccc
      601 ccccagcccc ggggccatgc cgaacaccag gctggtgacg cccgccatga tcgtgatggg
      661 gacgaaccgg gtcagggagt agaccgtcac ctccgaggcg gtgccggcga tccccagcac
      721 gatgatgtcc ccgcccatcg tgaccttcat cacgaagttc cagaccagga accaccagct
      781 gagcgagagg aaccggcgga ccgcgggcat gtcgggcttg gcgtagccga accacggcac
      841 gtagatccgg gtcagccaga agtaggtggc gccgatcacc gtggtcccgg cgaccgtggc
      901 caccgcgatg cccaccaggc cgaaccccat cacggccgcc accaccagca ggatgccctc
      961 gacgaactcc agggacgcgg tcaggcccat ccgcttgtag gcgacgttct gcccggccat
     1021 gacggcccag gggatgttcg tgatgccggc cacgatgatg tcggcgatca gcaccatccc
     1081 ggcgatgcgg atgatccacg ccccggccgg cggcacgtgc atccatcctg gggcgaacca
     1141 ggagatcgcc aggccgatcg ggagcaggat cggcaggaag atcagccaca cggcgacggc
     1201 gctgcccacg gcccggcgct tgtcctccag gtcggtcgag gactggcggt tggccacgaa
     1261 ccacttcagg gcctcggcgg gacggccccc ggccgggttg gtgtgcccga tcagccgctg
     1321 caggacctgc cagttgccga agcccgtgtc ccccaggtgg tgcaggagga tcgggttcac
     1381 caccagcccg gtgatggccc gggccgcgta gtcgagggcc tgggccaccg agttcaggga
     1441 cgccttcttg gtcaggctcc cgtccgagaa cagccgggtc ttggcccggg tcaccaggcc
     1501 gggacggggg gcctcgggcc gatcggccac ccgcaccagg gggtcctcgg ccaccgctac
     1561 agcccccccc agatcgcggc ccggatccgc gccagcacct cgtcccgagg acggccggcg
     1621 tcgatcacca gggccggggt gcggctccag tccagggccc agatctcctc cgcccggcgg
     1681 cggacgaacc cctcgctctc gaccccccgc cgccgtgcca ccgccacgtc cgggtccacg
     1741 cgcagcacga tcaggacgtc gggatagccg gcccgccggt agtagcgctc ctccatgcgg
     1801 gccaggatcg ccaccagggg gtggcggcga cgggaccggg acagccgggc cgtggcggga
     1861 ccgtccatct gggtgacctc ggccaggggg aaccggtcgc agatcacgat ggcgccttgg
     1921 gaggccaggc ggcgggcccg cacggtggcc aggtaccggt cccgcgaggt caggaccttc
     1981 cggacgagct tcagcagccg ccgcgtcgtc atcgggtcat cgtcggtgag ctcgaccggg
     2041 gtgcgcccct cgggggaggg agtgccctgg ggaccgcccc cggccccccg ccagacggcg
     2101 tggaccaggg ccgaggtcag cgagcggggc ggcttgccca ggtgcatcgc gatcgcgtcc
     2161 aggtcgcggg acagccagcg gtgcacctcc tccacggcgg tggtcttgcc ggcgccgtcg
     2221 ccgcccacga tcgcgatcag ggcgcccccg ccctccagcc ggctccgggg gggccgccgg
     2281 tgcaggatgc gccggtccag ggcccgtcgt ccccgccgcc acacccgcag gaacggatcc
     2341 agcgcgggcg agcgtctggc ctgcggagcg agcgcgcgct ccaggcgggc ggcggtccgc
     2401 gcccggacca ggggcgaggt accgggctcc aggcaccgca ggcatcgatc ccacagttcg
     2461 gggcccacgg agggcaggtg ctcgcgcacc agcccccgcc cctcctccga agtggccagc
     2521 gcacgcaggt acccgagctc gtgagcctca cccttggaca gcgatccctg gccggtcagc
     2581 atggcgtccg gcgtggagtg cttcagcacc atccgcagga cgaacacgac cagctcgaag
     2641 gccggcgccg gcacccggaa cagcggtccc tgctcggccg agaccaggta cgcccgctcg
     2701 accggcaggc ggtagttctt cgtcatgtcg tcgcccagca ccagctggtg gtgcacgtgc
     2761 aggtgcacca ccctcccgga gggctcgtcg aacccgtacg cgtggtacac gccgggcatc
     2821 cgctgcctgg cctggggcgg gcggccgcgc ttgaagccga ggtcgtggac gatccgggcg
     2881 aagcgctcgc cgtggtccgg gtccaccaac agatccaggt cgctctgggc cagcgccgag
     2941 cggtccaggg ccgcgttgct cttccagtgg cagtacgcga ccccgtcttc cgacagcctc
     3001 cgacacaggc gccgcaccag ttcgacggcc gtgggctcct cacccgccgg gggacgcgga
     3061 gcaggcggaa cgggcgccgg cgcgctcggc tcccggagcg gggaggagga cgcgaccagg
     3121 ccggcgcctg ccggttcgac cggagagacg tccctcaccg gccgatccgg gggaagcggg
     3181 gcggaccgaa cgttggctgg aacgggatga ggacctcctg ggctgccggg acgacacgac
     3241 cccctcgggt catgcccctc gacgatagga accggccgta cgtatcgcac gcgtgcggcc
     3301 gcgtatctct cctacgtcga acgcatgact ccccgggccc gtggaccggc cggcagacga
     3361 ctgggccccg gctcatcccc aacccgcacg ctccaccggc tccgcctccg cgggccgtcc
     3421 ggggatccac agccgggcgt cgggcagcac gtcatcgtcc cggtacgcgt ccagctcgaa
     3481 cagctcgagg atccgcatca cgcgggactc cgcgccgcga tcgaccttcc tccgccactt
     3541 ctccaccagg atgggccgat cgcccgcggc gccctcggtg agcagccgac gggtctcctc
     3601 cgccgacttg aaccgcacgt tcacgctcgg cacacccaga cggtcccgca tgcgctcggg
     3661 gctcggcagc gccagtttcg cgcagagctc ctggatcacc ggctcggggt ccaggaccag
     3721 ctgctcgtac gtgaccacgg cccagtccgg ggtggcttcc cgcaacgcga cggcgttctg
     3781 cagacaccag gacaggaccc cgcgctccag gtcggtgccc tgggcggcga tccgttcggc
     3841 ctcggtcagc tgctcggggc ggaagtgtcg gcggtagtcg ccgtgcagga acgtggggag
     3901 caccggccag cgctcgctgg agatcgccac ggcgatggga tggcgcagca ggtacaccac
     3961 ccgggcgtcg aacgtgtcgc ggatccagtt cacgcgatcc gccggcgcgt ggatctcctt
     4021 gaagacgatg cgatggctga tgggccggta gggggtccga agcgggctgg ggttcgtgaa
     4081 gccgatccgg ccggcgcaga tcctgcccag gtaccgctgg agcttctggg tgacctcggt
     4141 accgtagagc tcgtcccagt cccggatccc cgagcgccgc cggaccaccg ggttctcgag
     4201 gtacaggggc tggttgcagg tcttgaaccc cggctggctc cagatcagct ccatcagcca
     4261 ggtgctcccg ctcctaggcg tggagtacag gaacacgttg ggacggccgt ctgggcggtg
     4321 caggttcgag cccgccttca cggtgcgagc cacggccttg cggatcaccg ggcgctcctt
     4381 ccctcgatcc gccccgtcac cgccaggacc tgctgcgcga tctgctcggg ggcacgctga
     4441 tcgctacgga ccggcagcag cgccggcgcg gcgtctccgg ccatctccag gacctcggcg
     4501 aaggctcgtc ggtaccgctc caggaaggcc cgctgctgct ccaccgacgg ctccgagctc
     4561 aggtaccagt gcccccgccg ctcgacgcgg cccagcagca cctcgtccgg ggcatcgagc
     4621 gagacgatca ggtccagggc gtgggcccac ccgtccagca tcgtgcgcca ccaccgaccg
     4681 aaccgttcgc tgcgggcgag tggtggtccg aactcgcgca ggcgggccaa gcggtagatc
     4741 ggtccgtgat cgagcaccac gagcggcgac gccgaccagg tcccccgctg gacggcccgc
     4801 agccacgagt ccaggaaggc gatcgacttc atctcgttcc acgtgaacca gcggccgcgg
     4861 gggcgagcgc gcagccagat gggcaccagg ccggccagcc ggagcaccag gcgcgggaac
     4921 cagatccgcc gggggacccg cacccccgcc gcgacccgcc cctcccggga ccggagcgcc
     4981 cgggccaggg aggtcttccc ggcggtcgcc gggcccatga gctccaccag caccggccgg
     5041 tgccggacct cgggcccgtg ggcgcccgcc ccatcggcgg cggtcacgtc ggcgggaggg
     5101 gccggccggg tgcgggcgag gtccatgtgc agatactgta cggagcggtc caggaccgga
     5161 cacgcggccc cgagcgtatc tcgggtacgt cgagcgaacc agcggccgga ggcggtgcgg
     5221 cgaccgccct catcccctgc cctcggatcg acgatccccg ggggcgggtg atgccagccc
     5281 ggcgtaggcg cgctcggccg ccgcgcgaag cggggccaac gacggtcctc ggtacgcggc
     5341 ctggagctcc tccttggccg cccggcgctc ggccgtgggc cgctggaccc ggagctcggg
     5401 gatcgccggc cggcgcccct ccaggcggta ccgttcggtg gccgcccggt actggtccag
     5461 gctgatcagc cccgagacga accggttcag gtgcggctgc cagggcgggc cctcggcccg
     5521 ttcgtcgatc agggcgaaca cggcctccac gttctgctcg gtgtgctcga aggatcggaa
     5581 cgccctgccg aagcgctcgt tcacccgccg gatcacagct ccgaggtcgg tggtcacctc
     5641 ctcgaaggcg cccaccacga atccggagcg gaagggccgg atccgctcgt agaacgcgac
     5701 gtaggtcttg agccactgcg tcagggggat ccccggctcc cggacggcag ccgacaggat
     5761 cgtgggctcg ggctcccgca cggtcagcag cgccggcaca ccgcggcggg ccgccatcac
     5821 catggccgcc gcggcgtgca tgtggtgcgc cacgcggacg tggtcgttct gggcgaactg
     5881 gaaggcgatg gccgcgaacg tccccgcgga ccgggtgaag ccgtccacca gcagctcggt
     5941 ctgctcgccg agggccgtgt gatcgttctt ccaccgggcc agcggaaggt agacgcccgg
     6001 gcggagcgag atccagcggc gcaggacgtg gcgggaagcg gtgagcgccg agctctgccc
     6061 ggggcgtcga gtcggcacgg tccccatcgc cactcaggaa ccggtcagtc cacggtacag
     6121 cccggacagg tcgcggtaga aggcctcggc ggtgaagcgc tcgcgcaccc ggcgga
//
LOCUS       DASQQQ010000288         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_348984, whole genome shotgun sequence.
ACCESSION   DASQQQ010000288 DASQQQ010000000
VERSION     DASQQQ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348984"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VE646_10830"
     CDS             complement(<1..216)
                     /locus_tag="VE646_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80517.1"
                     /translation="MRSMVATLRLPQRCPSWLRMGTHSAAAHASGLGERPSTSAHERL
                     GVGILAEAISWALNLTPVPADRSERPSS"
     gene            complement(283..1560)
                     /gene="pfp"
                     /locus_tag="VE646_10835"
     CDS             complement(283..1560)
                     /gene="pfp"
                     /locus_tag="VE646_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HZD80518.1"
                     /translation="MKLGILCGGGPAPGMNSVISAATIEARNSGWDVLGIMDGFQHLI
                     EGRIEEARPLSITDVSRIHLQGGSIIRTSRANPTVRDEDAADPDWRLHACLESLQKLG
                     IDALVTIGGDDTAFSASRLAEAAGGGLRVAHVPKTIDNDLPLPGGAPTFGFETARSVG
                     VQLVNNLMADAMTTQRWYLVVAMGRSAGHLALGIGKSAGATLTVIAEEFPREEPIRLS
                     RLVDILETSMLKRIAHGRPFGVAVLAEGIGLRLPQEDLRKAMPEVERDEHGHVRLAEL
                     ELDRLLAKQVKDRFKERGENVTVEGKNIGYELRCAPPIPFDIEYTRDLGYGAVEYLKR
                     VLEGDEPGGMTTIQEGHMVPLPFGSFSDPETGRVRIRLVNIESNSYRVAREYMIRLDR
                     EDLEDPEKLRPIAEASGLTPEAFRDRYGYLAEG"
     gene            complement(1630..1758)
                     /locus_tag="VE646_10840"
     CDS             complement(1630..1758)
                     /locus_tag="VE646_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80519.1"
                     /translation="MSERARASSDRLDVAPGRRRSHARCAELNGRIAAAHVVPPGL"
     gene            1895..2104
                     /locus_tag="VE646_10845"
     CDS             1895..2104
                     /locus_tag="VE646_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80520.1"
                     /translation="MLYAYRGCPAAAGTGCQPGPDRFRIVTWPESAGNVPGQATLYDD
                     SPNGDYDVDRAEPEPLARGTITVLR"
BASE COUNT          298 a          779 c          731 g          318 t
ORIGIN      
        1 actggatggc cgctcgctgc ggtcggccgg gaccggggtc aggttgagcg cccaggaaat
       61 cgcttcggcg agtatcccca ccccgagccg ttcatgggcg gacgtggacg gcctttcccc
      121 gaggccggaa gcgtgcgcgg cggcgctgtg cgtccccatg cgcagccagc tcggacaacg
      181 ctgaggtaat ctcagcgtcg cgaccatcga ccgcatgcaa gtactgatac gaggcggccg
      241 ggttctctcc gacggggtcc ggggcggctc agtggccgag gctcacccct cggctagata
      301 gccgtaccgg tcgcggaagg cctcgggggt gagccctgac gcctctgcga tcgggcggag
      361 cttctccgga tcctcgaggt cctcgcggtc cagccggatc atgtactcgc gggccacccg
      421 gtacgagttc gactcgatgt tcaccagccg gatccgcacc cgccccgtct cggggtcgct
      481 gaacgacccg aagggcagcg gcaccatgtg cccctcctgg atcgtggtca tgccgccggg
      541 ctcgtcgccc tccagcaccc gcttcaggta ctcgacggcg ccgtagccga gatcgcgggt
      601 gtactcgatg tcgaacggga tggggggcgc gcaccggagc tcgtagccga tgttcttgcc
      661 ctcgaccgtg acgttctccc ctcgctcctt gaagcggtcc ttgacctgct tcgccagcag
      721 gcggtccagc tcgagctcgg ccagccggac gtggccgtgc tcgtctcgct cgacctccgg
      781 catggccttc cggagatctt cctgggggag ccgcaggccg atgccctcgg cgagcaccgc
      841 cacgccgaag gggcggccgt gggcgatccg cttgagcatc gaggtctcga ggatgtccac
      901 caggcgcgac agccggatgg gctcctcgcg ggggaactcc tcggcgatca ccgtgagggt
      961 ggccccggcc gactttccga tgcccagcgc caggtggcct gctgagcgac ccatggccac
     1021 caccaggtac caccgctggg tggtcatggc gtcggccatc aggttgttga cgagctggac
     1081 ccccacgctc ctcgcggtct cgaagccgaa ggtgggcgcg ccgcccggta gcggcaggtc
     1141 gttgtcgatg gtcttcggca cgtgcgccac gcgcaggccc cctccggccg cctcggccag
     1201 ccgggaggcc gagaacgcgg tgtcgtcccc gccgatcgtc acgagcgcgt cgatccccag
     1261 cttctgcagc gactcgagac aggcgtgcag cctccagtcc gggtcggcgg cgtcctcgtc
     1321 tcgcaccgtc gggttggccc gtgaggtccg gatgatcgac ccgccctgga ggtggatcct
     1381 cgagacgtcg gtgatggaga ggggccgcgc ctcctcgatc cggccctcga tgaggtgctg
     1441 gaagccgtcc atgatcccga gaacgtccca tccggagttc cgggcctcga tcgtggccgc
     1501 agagatgacc gagttcatcc cgggggcggg gccgccgccg cacaggatcc ccagcttcat
     1561 gccaccatcg ttccagcaac gaggaaatcc tgacaagcgg accggccccg gacctcggaa
     1621 ccatgcctgt cacaatcccg gcggcacgac gtgcgcggcc gcgatgcggc cgttcagctc
     1681 ggcgcaccgg gcgtgggagc gccgtcggcc cggcgccacg tccagccggt cgctggaggc
     1741 ccgggcccgc tcgctcatcg cccaccacct ggtcaaccgg ccctactgac cgggcggggg
     1801 cgtggggggg accgggcacc gttgcggtcc ccaccgtccc ggtcgccccc gatcgggcac
     1861 cggcgaactg gagcccggca ggcgagtcgg cttcgtgctc tacgcctacc gcggctgccc
     1921 cgccgccgcg ggcaccggat gccagcccgg ccctgaccgc ttccggatcg tgacctggcc
     1981 ggagtcagcc ggtaacgtgc ccggccaggc gacgctgtac gacgacagcc ccaacggcga
     2041 ctacgacgtc gaccgcgcgg aaccggaacc gctcgcccgg ggaaccatca ccgtcctccg
     2101 ctgacccgac cagggcaccc ggcggt
//
LOCUS       DASQQQ010000289         2489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_18592, whole genome shotgun sequence.
ACCESSION   DASQQQ010000289 DASQQQ010000000
VERSION     DASQQQ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18592"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="VE646_10850"
     CDS             <1..1001
                     /locus_tag="VE646_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80521.1"
                     /translation="GGADRDAETEARRPGGYEPGEPGGFGHSRSEPPGPHSPAGWPAG
                     PASGRPEPVARQPERPEPPPPEQVPPEPARASWDVPEPARDVPAGENPTHAQQDRDWS
                     PVRGRSDRDLPPAYQDDMAQEGTSDEDTGALGLGRPASAAERAQHGSEPSHALARVNG
                     EDRLPIFEAVESEWFRRRTSKRFASAPTPTPLPGEAPYPSQQGEPSRGGQRVGDAADP
                     ASPQAGSEAEQEPREWRSPGDEAWRAADSIRKPTTAGLTPAGLPIRIPKAHFVPGSAG
                     KSTLPTPARTPARSPEAVRGRLSSFHQGVRQGRDAGRNRGEENTHNAPRNQDEENT"
     gene            998..1420
                     /locus_tag="VE646_10855"
     CDS             998..1420
                     /locus_tag="VE646_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HZD80522.1"
                     /translation="MSELSQAAQNLNWLITNFVDRVPGVAHTIVVSADGLLLAVSEGF
                     PRDRADQLAAVASGLTSLTQGASRVFEGGTVTQTVVEMERGFLFIMSVSDGSCLAVLA
                     STACDIGLVGYEMALLVERAGEVLTPALRVELQGALPR"
     gene            1461..1796
                     /locus_tag="VE646_10860"
                     /pseudo
     CDS             1461..1796
                     /locus_tag="VE646_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004925001.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
     gene            1777..2373
                     /locus_tag="VE646_10865"
     CDS             1777..2373
                     /locus_tag="VE646_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010305137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HZD80523.1"
                     /translation="MDYASSRSAQPTESRTTTATSVKIVVAGGFGVGKTTFVGAVSEI
                     IPLTTEAVMTQASAGLDDLAATPNKTTTTVAMDFGRVSLEEDLILYLFGTPGQHRFWF
                     MWDDLVRGAIGAVVLVDTRRLADCFAAVDFFEECKLPFVVGVNCFDGILSHSVQDVRE
                     ALAISDHVPVVTCDARTRESTKSTLITLVEHAMAVHTG"
BASE COUNT          383 a          861 c          908 g          337 t
ORIGIN      
        1 gaggtggcgc ggaccgggat gccgagacgg aggcgcggcg acccggtggg tacgagcccg
       61 gtgagcccgg tgggttcggc cacagtcgct ccgagccgcc cggcccgcac tccccggcgg
      121 gctggccggc cggcccggcg agcggacgcc ccgagccggt ggcccgccag ccggagcggc
      181 ccgagccacc gccaccggag caggtgcctc cggaaccggc gcgggcgtcc tgggacgtac
      241 ccgagccggc cagggacgtg ccggccgggg agaatcccac tcacgcgcag caggaccgag
      301 actggtcgcc ggtccgcggg cgatccgacc gggacctgcc gcccgcgtac caggacgata
      361 tggcgcagga gggaacctcg gacgaggaca ccggtgcgct cggcctcggc cgcccggcgt
      421 cggccgcgga gcgtgctcag cacggttccg agccgtccca cgcgctcgca cgggtcaacg
      481 gggaggaccg gctgccgatc ttcgaggcgg tcgagtccga atggttccgc cggcgcacca
      541 gcaagcggtt cgcctcggcc cccacgccga ccccgctccc cggtgaggct ccctacccga
      601 gccagcaggg cgagccgtcc cgcggcggcc agcgggtcgg ggacgccgcc gacccggcgt
      661 ccccccaggc cgggtccgag gctgagcagg agccgcggga atggaggtcg ccgggcgacg
      721 aggcgtggcg ggcggcggac tcgatccgca agcccaccac cgccggcctg accccggccg
      781 gcctcccgat tcggatcccg aaggcacact tcgtaccggg ctcggccggc aagtcgacgc
      841 tgccgacccc ggcccgtacg ccagcgcgct cacccgaggc cgtccgggga cggctgagca
      901 gcttccatca gggcgtccgg caggggcgtg acgccggacg aaatcgcggc gaggagaaca
      961 cgcacaacgc cccccgcaac caggatgagg agaacacgtg agcgagttga gtcaggccgc
     1021 ccagaatctc aactggctga tcaccaattt cgtcgaccgg gtacccggtg tggcccatac
     1081 gatcgtggtc tcggccgacg gtctcctgct ggcggtctcc gaggggtttc cgcgtgaccg
     1141 ggccgaccag ctcgccgcgg tcgcctcggg cctgaccagc ctgacgcaag gcgcttcgcg
     1201 ggtgttcgag gggggcacgg tcacgcagac cgtggtggag atggagaggg gcttcctgtt
     1261 catcatgtcg gtcagcgacg ggtcctgcct ggcggttctc gcctccacgg catgtgacat
     1321 cgggctggtc ggatacgaga tggcgctgct cgtggagcgt gccggcgagg tgctgacccc
     1381 ggcgttgcgg gtcgagctcc aaggtgctct cccacggtga tggaagggaa accgcggtga
     1441 gtacgagcga gggtggcgat catggacccc tggtgcggcc gtacgccgtc accgggggac
     1501 ggaccaggcc ccggtaccac ctggccatcg aggctctggt ctcgaccagc gggtacgggg
     1561 aacatcatgc cccggggttg atcccggagt accagacgat ctgcaatctg tgcgtcgata
     1621 tccggtccgt ggccgagatc gcctcccatc tacaggttcc tctaggcgtg gcccgggtgc
     1681 ttgtcggaga catggcggaa gagggcctgg tcaaggtgca ccagcccgga aacgacgagg
     1741 ggcagccgga ccgtgcgctg ctcgaaaggg tgttgagtgg actacgcaag ctctaggtcg
     1801 gcccagccca ccgagtcgcg caccaccacg gccacctcgg tcaagatcgt ggtggccggc
     1861 ggcttcggcg tgggcaagac gaccttcgtc ggagcagtgt ccgagatcat cccgctgacc
     1921 accgaggcgg tgatgacaca ggcgagcgcc gggctcgacg acctcgcagc aacaccgaac
     1981 aagaccacca cgacggtggc catggacttc ggccgcgtgt cactcgagga agacctgatc
     2041 ctctacctgt tcggcacgcc cgggcagcat cgcttctggt tcatgtggga cgacctggta
     2101 cgcggggcca tcggcgcggt cgtgcttgtg gacacccggc ggctggccga ctgcttcgcc
     2161 gcggtggact tcttcgagga gtgcaagctg ccgttcgtgg tcggcgtcaa ctgcttcgac
     2221 gggatcctgt cgcacagcgt ccaggacgtc cgcgaggcgc tggcgatcag cgatcacgtg
     2281 cctgtggtga cctgcgacgc gcgcacccgc gagtcgacca agtccaccct gatcacgctg
     2341 gtcgagcacg cgatggcggt ccacaccggc tgaggccggg tacggcaccg gaccagaaga
     2401 ccgccggacc gctcggcacg ggcgatccgg cggctcagcg tgtggcctcg gcgcggtgca
     2461 gcgcgatcgc gtggcgcatc gcctcccgg
//
LOCUS       DASQQQ010000290         7334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_162615, whole genome shotgun sequence.
ACCESSION   DASQQQ010000290 DASQQQ010000000
VERSION     DASQQQ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7334
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162615"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1758
                     /locus_tag="VE646_10870"
     CDS             115..1758
                     /locus_tag="VE646_10870"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HZD80524.1"
                     /translation="MAGGEVLMFPWISLVTFLPVSGAIVLAFFPREAHRAIRTWAIVV
                     TVLTFGAALLMYGQFEPSRTGFQLVERATWIRSLHFEYFVGVDGVSVFFVLLTTLLMP
                     AGVLVSFPIDHQVKNFHLAFLVLETAMVGSFVALDLLLFFFFFEALLFPMYLVIGGWG
                     SERRIYAAVKFFLYTMAGSAFLLLGILFLYFRAGTELGQTSFDYTVLQQLSLPVSTAR
                     WLFLAFFAAFAVKTPLFPVHTWLPDAHTEAPTAGSLDLAAVVIKVGAYGFIRFNLGLF
                     PEAGWFFRDPILTLAVIGIVYGAVVAIVQTDLKRLVAYSSISHMGFVTLGIFALSLQG
                     MSGGVITMINHGLSTGGLFLLVGMLYERTHTRDLNELNGLGAIIPIFAGVFLFVALSS
                     IGLPGLNGFVGEFLVILGTFAVSKWFAVAAVSGVVLSAIYLLWGYQRAMHGPPATAPI
                     AGGAEDAPSGGVRHLWGHLRDLELREYFIIVPVMAAILFIGLYPKPILRTVEPAARHY
                     VTCVRQVHQMNVRGGYHPIPQGQNPCIYGTRGPDMGGSG"
     gene            1770..3248
                     /locus_tag="VE646_10875"
     CDS             1770..3248
                     /locus_tag="VE646_10875"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HZD80525.1"
                     /translation="MVLPLAASHPPLSFGPLAPELTLVGTGLVLMVLGSLRPRVDQRA
                     MVLLSLAGIVGATAAAVRLWFWSGPVEVLGGMVATDHYAVFFRIVILAVSGLAVLLSF
                     HYLERTGEARIEYYPLLLFATAGMTLIGSATDLILVFLALEILSLALYLLTGFSFRRL
                     ASTEASIKYFLLGAFSSAFFLYGIAMAYGATGSTHLSTIAGKLSGRPGSDAIAMTAFA
                     LLIIGFGFKVAAVPFHMWTPDAYQGAPTCVTAFMSAGTKVAAFAAFIRVFSTAFQPLT
                     LDWTPIVWILAAVTMVVGSVLAIAQTDVKRLLGYSSITQAGFVLVGMTAGPQGISGAM
                     FYLAAYAAMILGAFGVVMLVSVRGEARTSMSSYAGLARRNPYAAAVLAILLLSLAGIP
                     PMAGFIGKVSVFSAAIQAGHWPLALLGVLASVIAAFYYIRVIVVMYMQEYLPEPEEVP
                     GFERAPVARLAVTIPTAAVVVFGIFPGIIFGFLRTASILHVR"
     gene            3300..4307
                     /locus_tag="VE646_10880"
     CDS             3300..4307
                     /locus_tag="VE646_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZD80526.1"
                     /translation="MSRRVIPGLEAPDPVLEREVRSRLDEVEAALEKSVRADSDLLAE
                     TSSYLLSAGGKRFRPMLVLLGGYFGDPRDPRLVPGSVAIELVHLATLYHDDVIDEADS
                     RRGIPSVNARWDNTVAILTGDYLFARASEISTELGTAVIALLARTIATLCDGQIREVE
                     VSGRLDQRDEIYMEIIRRKTGALIATSCRLGGLLSDAPLELLDVLEGYGEALGMAFQL
                     SDDIMDVTATHVELGKEPGVDMREGVYTLPVLHALSRGERRQELAALLSEGPPEDVRL
                     ERALEIVRSPESLDHARAAVAEEVDRAVGFALRLPHGTARSALVTLAEFLAARCGARQ
                     A"
     gene            4324..4722
                     /locus_tag="VE646_10885"
     CDS             4324..4722
                     /locus_tag="VE646_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014047737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZD80527.1"
                     /translation="MAELEKAFTSVAETYYQSYGVVLAVLIAGAALVAVAFGAARLLA
                     PRRPLPAKLTTYECGIDPVGIGWAQSQVRYYIYGFLFVIFDVESVFLFPWARVFESLG
                     YQAVVEMAIFIGILAVGLLYAWRKGVLRWA"
     gene            4737..5225
                     /locus_tag="VE646_10890"
     CDS             4737..5225
                     /locus_tag="VE646_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019354190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HZD80528.1"
                     /translation="MPEIQPVKFLLNWGRRYSLWVMNFGLACCAIEFIAASTSKHDFI
                     RLGVIPFAHGPRQADLLVVAGTLTDKMAPALKRVYDQMPDPKYVISFGSCSNCGGPYW
                     DSYSVTKGVDQIVPVDVYVPGCPPRPEALLHGIIKLQEKIGKEDIREKWSGKLITPTA
                     TS"
     gene            5191..5712
                     /locus_tag="VE646_10895"
     CDS             5191..5712
                     /locus_tag="VE646_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZD80529.1"
                     /translation="MASSSRRLPPPEIESRLRAQFGDDVLAFEDQSGHAVVTIPAERY
                     RELVTFLRDEPEFACDYCDFTGGVDLPDRFEVVTHLFSTRHHHHVRLKVPLPREDPAC
                     PTISDLFPTANWHEREIMEMFGIDFPEHPQPVKLLLPEPFEGHPLRKDFPLATREAKP
                     WPGEAEGEEEEEA"
     gene            5718..6707
                     /gene="nuoH"
                     /locus_tag="VE646_10900"
     CDS             5718..6707
                     /gene="nuoH"
                     /locus_tag="VE646_10900"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010056653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HZD80530.1"
                     /translation="MPGVSVFNDVFSNLWVALIVKTVVVIGFFLVAPLLVGYAEHKVL
                     AHMQARLGPMYAGRFHGWAQLVADGVKFVQKEDVIPDAADPWVFSLAPAVALIPYIAI
                     FVVVPFSNTIFAENLDIGIFFVLAIGSVNVIGVLMAGWASANKFSLIGALRAAAQLIA
                     YELPLVLAAASVVMQAGTLSLIGISEAQDRYWFVLTQPLGFLIFMVAALAELTRPPFD
                     MPIADSEIIFGAYTEYTGLKFAFFLLSEYGGIVALSAIASILFLGGYRGFLGIPDPLW
                     MAAKIGALSFVVIWLRATYPRLREDQLQRFSWLALIPLALLQILVVAVVKVAV"
     gene            6779..>7334
                     /locus_tag="VE646_10905"
     CDS             6779..>7334
                     /locus_tag="VE646_10905"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017239496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HZD80531.1"
                     /translation="MGQGLIKGLGVTLKHMLRPAVTQQYPEVKPDLPARTRGVIALKQ
                     ANCTVCYKCSRECPDWCIYIDAHKETHPPASGGRARSVKVLDRFDIDYALCMYCGICV
                     EVCPFDALFWSPEFEYATYDIHDLLHDKEKLEEWTYTVLPPPPLEEGAEPPPEEPETA
                     AAQAAAAPAPAPAPAAVAPAPTEAP"
BASE COUNT          962 a         2491 c         2532 g         1349 t
ORIGIN      
        1 gaacggggtg ggctgggtgg tccgcacctc ggctgccgcc ggccggaggg tgcagacggg
       61 gttcgtgcgc aactacgggc tggcgttcct ggtgggtgtg gtggggatcc tgtggtggct
      121 ggcggtgagg ttctgatgtt cccgtggatc tcgctggtca cgttcctgcc cgtgtcgggc
      181 gcgatcgtcc tggcgttctt ccctcgcgag gcccaccgag ccatccggac ctgggccatc
      241 gtcgtcaccg tgctcacgtt cggggccgcc ctgctcatgt acgggcagtt cgaaccgtcc
      301 cggacggggt tccagctggt ggagcgggcg acctggatcc ggtcgctgca cttcgagtac
      361 ttcgtggggg tggacggggt cagcgtcttc ttcgtcctgc tcaccacctt gctgatgccc
      421 gccggcgtgc tggtctcctt ccccatcgac caccaggtga agaacttcca cctggcgttc
      481 ctggtgctgg agaccgcgat ggtgggatcg ttcgtggccc tggacctgct gctgttcttc
      541 ttcttcttcg aggcgctgct gttcccgatg tacctggtga tcgggggctg gggcagcgag
      601 cggaggatct acgcggcggt caagttcttc ctgtacacga tggccggctc ggccttcctg
      661 ctgctcggga tcctgttcct gtacttccgg gccgggaccg agctcggcca gacctccttc
      721 gactacacgg tgctgcagca gctctcgctg ccggtgagca cggcccggtg gctgttcctg
      781 gccttcttcg ccgcgttcgc cgtgaagacg ccgctgttcc ccgtgcacac ctggctgccc
      841 gatgcccaca ccgaagcgcc caccgccggg tccttggacc tggccgcggt ggtgatcaag
      901 gtgggcgcgt acggattcat ccggttcaac ctggggctgt tccccgaggc cggctggttc
      961 ttccgcgacc ccatcctcac cctcgccgtg atcgggatcg tctacggcgc ggtggtcgcc
     1021 atcgtgcaga ccgacctgaa gcgcctggtc gcctattcga gcatctcgca catggggttc
     1081 gtcacgttgg ggatcttcgc cctcagcctg caggggatgt cgggcggggt gatcacgatg
     1141 atcaaccacg ggctgtccac gggcgggttg ttcctgctcg tgggcatgct ctacgaacgc
     1201 acgcacaccc gcgacctgaa cgagctgaac ggcctcgggg cgatcatccc gatcttcgcc
     1261 ggcgtgttcc tgttcgtggc gctgtcctcg atcggcctgc ccggcctgaa cggcttcgtg
     1321 ggcgagttcc tggtgatcct gggcacgttc gcggtgagca agtggttcgc ggtggccgcg
     1381 gtctccggcg tggtgctgtc cgcgatctac ctgctctggg gctatcagcg cgcgatgcac
     1441 gggccgcccg cgaccgcgcc catcgccggt ggggcggagg atgccccgtc cggtggggtc
     1501 cgacacctgt gggggcacct gcgggacctg gagctccgcg agtacttcat catcgtcccg
     1561 gtgatggccg cgatcctgtt catcgggctg taccccaagc cgatcctgcg gaccgtcgaa
     1621 cccgcggctc gccactacgt cacctgcgtg cggcaggtcc accagatgaa cgtccggggc
     1681 gggtaccacc cgatccccca aggtcagaac ccctgcatct acgggacgcg gggccctgac
     1741 atgggggggt cgggatgatg gtcggaccga tggtcctgcc gctggccgcc agccatccac
     1801 cgctgagctt cgggcccttg gcccccgagc tgacgctggt ggggacgggc ctggtgctga
     1861 tggtgctggg gtcgctccgc ccccgggtgg accagcgggc gatggtgctc ctgtccctgg
     1921 cgggcatcgt cggggcgacc gccgcggcgg tccgcctgtg gttctggtcc ggtccggtcg
     1981 aggtgctcgg cgggatggtg gccaccgacc actacgccgt cttcttccgg atcgtgatcc
     2041 tggccgtgag cgggctcgcg gtgctgctgt ccttccacta cctggagcgc acgggcgagg
     2101 cacggatcga gtactacccg ttgctgctgt tcgccacggc ggggatgacc ctgatcgggt
     2161 ccgccaccga cctgatcctg gtgttcctgg ccctggagat cctctcgctc gccctgtacc
     2221 tgctcacggg gttctccttc cgtcggctgg cctccaccga ggcctcgatc aagtacttcc
     2281 tgctgggcgc cttctcctcg gcgttcttcc tgtacgggat cgcgatggcg tacggagcca
     2341 ccggcagcac gcacctgtcc acgatcgcgg ggaagctctc cggtcggccg ggatccgatg
     2401 cgatcgcgat gaccgccttc gccctgttga tcatcgggtt cgggttcaag gtggcggcgg
     2461 tgccgttcca catgtggacc ccagacgcct accagggcgc tcccacctgc gtgacggcgt
     2521 tcatgtcggc ggggacgaag gtggcggcct tcgccgcgtt catccgcgtc ttctccacgg
     2581 cgttccagcc cctcaccctg gactggacgc cgatcgtctg gatcctggcg gcggtcacca
     2641 tggtcgtggg cagcgtcctg gccatcgccc agaccgacgt gaagcggctg ctcggatact
     2701 cctcgatcac ccaggccggg ttcgtgctgg tggggatgac ggcgggtccc caggggatct
     2761 cgggggcgat gttctacctg gccgcgtacg ccgcgatgat cctcggcgcg ttcggcgtgg
     2821 tgatgctggt ctcggtccga ggggaggcgc gcacctcgat gtcctcctac gccggcctgg
     2881 cgcgccgcaa cccgtacgcg gccgcggtcc tggccatcct cctgctgtcg ctggccggga
     2941 tcccgccgat ggccggcttc atcggcaagg tctcggtctt cagcgcggcg atccaggccg
     3001 gacactggcc gctcgcgctg ctgggcgtgc tggcctccgt gatcgccgcc ttctactaca
     3061 tccgggtcat cgtggtgatg tacatgcagg agtacctgcc cgagcccgag gaggtccccg
     3121 ggttcgagcg ggccccggtg gcccggctgg ccgtgaccat ccccaccgcc gcggtggtgg
     3181 tcttcggcat cttccccggc atcatcttcg ggttcctgcg gaccgcgtcg atcctccacg
     3241 tgcgatgagg acggggatcc catggtgagg ggcaagctcc ggcggctaga gtcatccgga
     3301 tgagcaggcg cgtgatcccc gggctggaag ctcccgatcc cgtcctggag cgtgaggtcc
     3361 ggagccggct cgacgaggtg gaggccgcgc tggagaagtc ggtccgggcc gattccgacc
     3421 tgctggcgga gacctcctcc tacctgctct cggccggggg caagcggttc cggccgatgc
     3481 tcgtgctgct cggtggctac ttcggcgatc cccgggaccc tcggctagtc cccgggtcgg
     3541 tggccatcga gctggtgcac ctggccaccc tgtaccacga cgacgtgatc gacgaggccg
     3601 actcccggcg ggggatcccc tcggtgaacg ctcggtggga caacaccgtg gccatcctga
     3661 ccggggacta cctgttcgcc cgggcctcgg agatctccac cgagctcggg accgccgtga
     3721 tcgccctgct ggcccggacc atcgccaccc tgtgcgacgg gcagatccgc gaggtggagg
     3781 tctccggccg cctggatcag cgcgacgaga tctacatgga gatcatccgg cgcaagaccg
     3841 gggcgctgat cgccacctcc tgccgcctgg gcggcctgct ctcggacgcg ccgctggagc
     3901 tgctggacgt gttggagggc tacggggagg ccctgggcat ggccttccag ctctcggacg
     3961 acatcatgga cgtcacggcc acccacgtgg agctgggcaa ggagcctggg gtggacatga
     4021 gggaaggggt ctacacgctg ccggtcctgc acgcactgag ccggggggag cgacgccagg
     4081 agctggccgc cctgctctcg gaggggcctc cggaggacgt ccggctggag cgggccctgg
     4141 agatcgtccg ctctcccgag agcctcgacc acgcccgggc cgccgtggcc gaggaggtgg
     4201 accgggccgt ggggttcgcc ctgcgcctgc cccacgggac ggcccggtcg gcgttggtca
     4261 ccctggccga gttcctggcc gcccgatgcg gagcgagaca agcgtgagga cggccttgct
     4321 agaatcgccg agcttgagaa agcgttcaca agcgtggcag agacctacta ccagtcctac
     4381 ggggtcgtcc tcgcggtcct gatcgccggc gccgcattgg tcgccgtcgc tttcggagcc
     4441 gcccggttgc tggcaccccg tcgtccgctg ccggccaagc tcaccacgta cgagtgcggg
     4501 atcgacccgg tgggcatcgg gtgggctcag agccaggtcc gctactacat ctacggcttc
     4561 ctgttcgtga tcttcgacgt ggagagcgtc ttcctgttcc cctgggcccg ggtgttcgag
     4621 agcctgggct accaggcggt cgtggagatg gccatcttca tcgggatcct cgcggtcggg
     4681 ctgctgtacg cgtggaggaa gggggtcctg cggtgggcct gatcgagcgg accccgctgc
     4741 cggagatcca gccggtgaag ttcctgctga actggggccg gcgctactcg ctgtgggtca
     4801 tgaacttcgg cctggcctgc tgcgccatcg agttcatcgc cgcctccacc tccaagcacg
     4861 acttcatccg cctgggggtg atccccttcg cccacggccc ccggcaggcg gacctgctgg
     4921 tggtggccgg gacgctcacg gacaagatgg cgcccgccct caagcgtgtc tacgaccaga
     4981 tgccggaccc gaagtacgtg atctccttcg gctcctgctc gaactgcggc ggtccctact
     5041 gggactcgta ctcggtgacc aagggcgtgg accagatcgt cccggtggac gtgtacgtgc
     5101 cgggctgccc gccccggccc gaggcgctgc tgcacgggat catcaagctg caggagaaga
     5161 tcggcaagga agacatcagg gagaagtgga gtggcaagct catcacgccg actgccacct
     5221 cctgagatcg agtcccgcct gcgggcgcag ttcggtgacg acgttctggc cttcgaggac
     5281 cagagcgggc acgccgtggt gacgatcccg gccgagcggt accgggagct ggtcaccttc
     5341 ctgcgggacg agccggagtt cgcctgcgac tactgcgact tcacgggggg cgtggatctc
     5401 cccgaccggt tcgaggtggt cacccacctg ttctccacgc ggcaccacca ccacgtccgg
     5461 ctgaaggtcc ccctgcccag ggaggacccg gcctgcccca ccatctccga cctgttcccc
     5521 acggccaact ggcacgagcg ggagatcatg gagatgttcg ggatcgactt ccccgagcat
     5581 ccccagccgg tgaagctcct gctccccgag cctttcgagg ggcacccgct gcgcaaggac
     5641 ttccccctcg ccacccgtga ggccaagccc tggcccggcg aggcggaggg cgaggaggaa
     5701 gaggaggcgt gatcgggatc ccgggggtca gcgtcttcaa cgacgtgttc tccaacctgt
     5761 gggtcgccct gatcgtcaag accgtggtgg tgatcggctt cttcctggtc gcgccgctgc
     5821 tggtgggcta cgccgagcac aaggtgctgg cccacatgca ggcccgcctg gggccgatgt
     5881 acgcggggcg gttccacggg tgggcccagc tggtggccga cggcgtgaag ttcgtccaga
     5941 aggaggacgt gatccccgac gcggccgatc cttgggtctt ctcgctggcc ccagccgtgg
     6001 cgctgatccc ctacatcgcg atcttcgtgg tggtgccgtt ctcgaacacg atcttcgcgg
     6061 agaacctcga catcgggatc ttcttcgtgc tggccatcgg atcggtcaac gtgatcggcg
     6121 tgctgatggc ggggtgggcc tcggccaaca agttctcgct gatcggggcg ttgcgcgccg
     6181 ccgcccagct gatcgcctac gagcttccgc tggtgctggc ggcggcctcg gtggtcatgc
     6241 aggccggcac cctgtcgctg atcgggatct ccgaggctca ggaccgctac tggttcgtgc
     6301 tgacccagcc gctggggttc ctgatcttca tggtggccgc gctggccgag ctcacgcgtc
     6361 cgcccttcga catgccgatc gccgactccg agatcatctt cggcgcctac accgagtaca
     6421 cggggttgaa gttcgccttc ttcctgctca gcgagtacgg cgggatcgtg gccctgtcgg
     6481 ccatcgcgtc gatcctgttc ctgggcggct accgcgggtt cctcggcatc cccgatccgc
     6541 tctggatggc ggccaagatc ggtgcgctat cgttcgtggt gatctggctg cgggcgacgt
     6601 acccgcggct ccgcgaggat cagctccagc gcttctcctg gctcgcgctg atcccgctcg
     6661 cgctgctgca gatcctggtg gtggctgtgg tgaaggtcgc ggtctgagcg gaaggggtga
     6721 gacgaccgtg gcgaccgacg agcaggacct gaggaggccc cccaaggatg ctcccgcgat
     6781 gggccagggg ctcatcaagg ggctgggcgt cacgctgaag cacatgcttc gtccggccgt
     6841 cacccagcag tacccggagg tcaagcccga cctgcccgcc cggacccggg gcgtgatcgc
     6901 gctgaagcag gcgaactgca ccgtctgcta caagtgctcg cgcgagtgcc ccgactggtg
     6961 catctacatc gacgcccaca aggagaccca ccccccggcg tccggtgggc gggcccggtc
     7021 ggtgaaggtg ctcgaccgtt tcgacatcga ctacgccctg tgcatgtact gcgggatctg
     7081 cgtggaggtc tgccccttcg acgccctgtt ctggagcccc gagttcgagt acgccacgta
     7141 cgacatccac gacctgctgc acgacaagga gaagctcgag gagtggacgt acacggtgct
     7201 gcctccgccg cccctggagg agggggccga gccgcccccg gaggagccgg agaccgcggc
     7261 cgcccaggcc gcggccgccc cggcgcctgc tccggccccg gcggcggtcg ctcctgcccc
     7321 gaccgaagct cctg
//
LOCUS       DASQQQ010000291        10931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_18972, whole genome shotgun sequence.
ACCESSION   DASQQQ010000291 DASQQQ010000000
VERSION     DASQQQ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10931
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18972"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..317)
                     /locus_tag="VE646_10910"
     CDS             complement(39..317)
                     /locus_tag="VE646_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011936201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80532.1"
                     /translation="MGCLLVLFGLATPRLVMVLLWLFTGYLERAFGTWVWPLLGFFFL
                     PTTTIAYAIAKNAFAGVRGWGLVVVVLGVAIDFGLLGSARGRGLGRRD"
     gene            complement(317..1084)
                     /locus_tag="VE646_10915"
     CDS             complement(317..1084)
                     /locus_tag="VE646_10915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZD80533.1"
                     /translation="MDRVIVITGGAGGMGRATAARFLRRSDTVVLADVSDEGLAEAAA
                     ALEPAGGTLDRVIADVTRVEACERMIEHAVSAHGRLDVLVNCAGVWVEGPSELATEGQ
                     WDRTIDVNLKGTFFACRFAIPHLIETEGCIVNLSSDAGLVGTAEAAIYCASKGGVSNL
                     TRALALELAPRGVRVNAVCPNDVETPMLAGQARDYGGGDPDGYLRALLAKYPQAERAR
                     FIRPDEVAALIEYLASAEAAPITGACVPIDFGSTAGY"
     gene            1121..2434
                     /locus_tag="VE646_10920"
     CDS             1121..2434
                     /locus_tag="VE646_10920"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD80534.1"
                     /translation="MSRRPGRGPDTSSRRGGLIHRVAVDVQPLRVSRDFRLIWSGMLV
                     SELGYQFTLVATFFQVKELTGSTAAVGLIGLVGLVALVGGTLAGSSIVDRYDRRILLI
                     LSQIGYMLGSGILFVGALHGRPPLWLVYGGIAMIAAVSAVDGPTRSAMIPRLVGRDLL
                     PAALALGQVVWNGTALLGPALAGIVIGASERYGFSIAYGIDLATYGAILAAAFLIRPM
                     PPERDTAALGGAAAVKEGFAYLRGRRVLQSTFLIDLIAMIFGMPRALFPFLAASQFQA
                     GSQAAVSRIAGFLFAAPAVGAMVASAASGWVGRVRHQGRAVLMSVALWGLGIAAFGLM
                     GDRLLAALAALAFAGGADVISAVFRNTILQLSVPDSLRGRMSAIHILVVTGGPRLGDL
                     EAGLVAQAFTPMASVVSGGLLCLAGTGVLALLVPAFRRYRAGEAA"
     gene            complement(2443..2778)
                     /locus_tag="VE646_10925"
     CDS             complement(2443..2778)
                     /locus_tag="VE646_10925"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HZD80535.1"
                     /translation="MGVDGVRETLQERRSELRRELAALTEAPRDPLAAVSFGKRIGDG
                     TTEAVERLATTSAARSLFAKLTDVERALIKVEEGTYGRCDVCEGPIGAERLEAVPWAT
                     RCVRCPAKA"
     gene            complement(2781..3062)
                     /locus_tag="VE646_10930"
     CDS             complement(2781..3062)
                     /locus_tag="VE646_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80536.1"
                     /translation="MIEFTEWATQILRRTHLAARRFNPRASVRLQRRGDGVEFALTDE
                     AHEGDRLVEGDGFELLVESGIRGVVDVVEPHDQLILRPPGSTERSVREH"
     gene            complement(3102..3518)
                     /locus_tag="VE646_10935"
     CDS             complement(3102..3518)
                     /locus_tag="VE646_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80537.1"
                     /translation="MREDEDASGASTVGGRLRGPSPQAASAWLGAVLLALGVAGTLWS
                     SGLGAALPPAPAVVAGHWAIGLILIVGGFATDGTARVLLAVCGLFLVAAGVTGFVAAG
                     AVGEALGLFGPVPVAAWLLHLALGVAGLGGAALRRR"
     gene            3586..4509
                     /locus_tag="VE646_10940"
     CDS             3586..4509
                     /locus_tag="VE646_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HZD80538.1"
                     /translation="MPTILEFPLAGGGGEPVDLRRTLNSHGFVDLPPMRLRPDGSSLD
                     ITLVAGRAIRTVRVGPGGGGRGRITILGPAVGASTGKRLLASVRHVLRLDEDLSRFYD
                     LAAADPDLSWVALGAGRMIQAPTVFEDVVKTICTTNCSWSGTTRMVTALVEHLGAPAT
                     GAPAGEAADGAYGRAFPGPEAMADAGPDFYRQVARAGYRGAYLIGLARAVAEGELDLE
                     GLRDPALPDEEVERRLLDLPGVGPYAAAHIMMTIGRYHRLILDSWTRPTYAKLVGRRV
                     VKDAVMERRFRRFGPYAGLAFWLYLTRDWVS"
     gene            4633..5871
                     /locus_tag="VE646_10945"
     CDS             4633..5871
                     /locus_tag="VE646_10945"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HZD80539.1"
                     /translation="MARFPRRARRLWAYWRAEQRTLRQGFVALFISSGGDLLAGLALA
                     FMTDSLQRLPGLFLLIPAAIGMRGNIFGALGSRLGTGIHSGQFEPSADRRGFLGQNVM
                     ASVFLTLSTSLFLGAAARAATAAFGFATISIWDYLVISVVGGVLGSLVVGGASIGIAV
                     WSHRRAWDLDSVSAPLVTAIGDIATLPALWAASFLVQIRYVTLVLGIAVALVCLAATA
                     RGILYHLPLTRRIVIESMPVLVLAGIVDILAGTVIEARLERFITFPALLVLVPPFLED
                     TNALSGILSSRLASKLHLGVLEPRSVPQPLALVDISINFLFAISVFLMVGVSAELVSL
                     AAGKLSPGLGTMIGISLAAGFLATIASSAVAYYSAIATYRFGFDPDNHGIPIGSSVMD
                     LAGTLCLIAAILMFSVTAHG"
     gene            5864..7063
                     /locus_tag="VE646_10950"
     CDS             5864..7063
                     /locus_tag="VE646_10950"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HZD80540.1"
                     /translation="MADDEPRNVKELLVEAKDASELLIDMAYAAVFFNEEDLAGEVEK
                     LEERMDGYLRRLRTLAILAARSPEDAEAIEPVLWIAAAIDKIGDAASDIGRVVAADLG
                     IPDGLRKDLRHADEVSGRVKVREGSQSVGASLRDLSLPTETGMWVLAIRRGREWEFDP
                     GPDTVVSEGDVLLYQGPEEGVNLIREVAGAPPLPSAPESEPPPLSELDRAVDILVEMK
                     NISEVAVGLAYSCLLFNDRALAAEVATLEARSDMLHDELESWVLRASPEARNPDDLRG
                     LLRLGAASEAICDAARDMTWYVEHGEELHPVIQMALEETEETATETIVDEGSQAAGRS
                     LRDLQLETETGMFVLAVQRGRRWIYHPRGRFTLQAGDRIIAVGPEEGGEALQALCRST
                     REAVETG"
     gene            complement(7151..8080)
                     /locus_tag="VE646_10955"
     CDS             complement(7151..8080)
                     /locus_tag="VE646_10955"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HZD80541.1"
                     /translation="MRVVPRKILLAAPRGYCAGVERAVEAVERALARYGPPVYVRKQI
                     VHNLHVVRDLEGKGAVFVEDERDVPAGGVAVLSAHGVAPEVYANARDRGLTLLDATCP
                     LVTKVHLEARRFAADGRTIVLIGHAGHEEVVGTVGQAPTRIVLVQNPEEARRVPLDAS
                     DGVSYLTQTTLSVDETNEIIQILRDRFPAIEGPPREDICYATQNRQDAVKEVARRSDV
                     VLVLGSRNSSNSNRLVEVAKQQGARAYLVDDQTEVQAWWLEGADVVGVTSGASAPEWL
                     VERMLAFLREHGAAEVEEMRLTQERLRFSDVRV"
     gene            8430..8858
                     /locus_tag="VE646_10960"
     CDS             8430..8858
                     /locus_tag="VE646_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80542.1"
                     /translation="MAYLRHRRRVLLAVLTSVALLAMPATPVQARERSQVPRWRCRID
                     WQHGPWHVKKLIRCAARHWRVPGGPDKAVSVARCESRLDPSAYNAGGFAGVFQQSTRY
                     WRGRARTYGFPAHSVFNGRANVIVSVRMAHRYGWGPWGCA"
     gene            complement(8872..9174)
                     /locus_tag="VE646_10965"
     CDS             complement(8872..9174)
                     /locus_tag="VE646_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80543.1"
                     /translation="MAPDRQALADLKRAAVAADWELAPGTTDERDAMAQLAAVRPQVL
                     VVFGASEQLVSDARSAYPSLRIVADRALPGATVVVTSLREVRGAIQGLPRPGGPVR"
     gene            complement(9203..10171)
                     /gene="corA"
                     /locus_tag="VE646_10970"
     CDS             complement(9203..10171)
                     /gene="corA"
                     /locus_tag="VE646_10970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HZD80544.1"
                     /translation="MITCRVYRSGTLRDEGAFDPGAVEDARARGDRLWLDVIDPTGEE
                     LQQLQHTFDLHELSVEDSYRWGQRSKVEFYQGYVFIVVHALQVGEDDQLSDGEIHLFV
                     GGGSYVITVRRPPLFDLARVGERLAKEPDLVHEGVGYHLYLLLDEIADGYLDAVDKLE
                     DSSDDIELRVFQDEPAAELQADIFRLRRTAVRFRRLAAPMRQVVDLIQETPALVTPAL
                     APYYRDVQDHVIRSIEFIDNIRDLLTSAFESELAQVSNRLNAVMKQLTSWAAIILIPT
                     LIAGIYGMNFRDMPELRSAWGYPMALGAMVVSALVLYVVFKRREWL"
     gene            10216..>10931
                     /locus_tag="VE646_10975"
     CDS             10216..>10931
                     /locus_tag="VE646_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZD80545.1"
                     /translation="MSDLEPLLRFWRALDGRFGGVHPTPWGSVVTDARFPAIWDVNYA
                     RLEADHPIALSEVERSLLPALRRARAPRIHIVAFHPEVQTDLVTEASTRGDRLSWDSI
                     MVLRRPVDRGSTVAVEEIAGFDERFWRDVAGSMAEFDVTEADAVDQILRIEREILGPA
                     GKRWFSVRSGHRSVALGCLLVLEGVGLVDHVVTLPRFRGRGYASAIVGRIVQEARTSD
                     VEALWLLCEPDSRAEALYRRL"
BASE COUNT         1523 a         3919 c         3816 g         1673 t
ORIGIN      
        1 gcacgccatg ccgatcgcac ggggccgggg tcctgctatc agtcccgccg gcccaggccg
       61 cgcccgcgcg cgctgccgag cagaccgaag tcgatcgcca cgccgagcac cacgaccacg
      121 agcccccacc cgcgcacacc ggcgaaggcg ttcttcgcga tcgcgtacgc gatcgtggtg
      181 gtcggcagga agaagaagcc caggagcggc cagacccagg tgccgaaagc gcgctccagg
      241 tagccggtga agagccagag gagcaccatc accagcctgg gcgtggccag gccgaacagg
      301 accaagaggc agcccatcag taccccgccg tgctgccgaa gtcgatcggg acgcaggcgc
      361 ccgtgatggg ggcggcctcg gcagacgcca ggtactcgat cagcgccgcc acctcgtcgg
      421 gacggatgaa ccgggcccgc tcggcctgcg ggtacttcgc gagcagggca cggaggtagc
      481 cgtcgggatc gccgcccccg tagtcgcgtg cctgcccggc cagcatcggc gtctcgacat
      541 cgttcggaca gacggcgttc acgcggaccc cccgcggtgc gagctccagg gcgagggccc
      601 tggtgaggtt gctcacccca cccttcgagg cgcagtagat cgccgcctcg gccgttccca
      661 ccaggccggc gtcagacgag aggttgacga tgcagccctc ggtctcgatc aggtggggga
      721 tcgcgaaccg gcaggcgaag aacgtgccct tcaggttcac gtcgatcgtc cggtcccact
      781 gtccctccgt ggcgagctcc gagggaccct ccacccaaac cccggcgcag ttcaccagga
      841 cgtcgagccg tccgtgggcg gagaccgcgt gctcgatcat gcgctcgcag gcctcgaccc
      901 gggtgacgtc ggcgatcacc cgatcgaggg tccctccggc cggctcgagc gcggccgcgg
      961 cttcggccag gccttcgtcg gagacatcgg ccaggaccac ggtgtcggaa cggcggagga
     1021 agcgcgccgc cgtggcccgg cccatgcccc cggccccccc ggtgatcacg atcaccctgt
     1081 ccacggtcgg gcatcctagc ctccctgcta acgtcggcgc atgtcgaggc ggccgggtcg
     1141 agggccggac acctcttccc gccgaggagg gctcatccac cgggtcgcgg tggacgtgca
     1201 gcccctacgg gtctcgcgtg acttccggct gatctggagc gggatgctcg tctccgagct
     1261 cggctaccag ttcacgctgg tggccacgtt cttccaggtc aaggagctca ccggctccac
     1321 ggcggccgtg ggcctgatcg ggctggtggg cctggtcgcg ctcgtgggcg gcacgctcgc
     1381 cggaagttcg atcgtggacc gctacgaccg ccggatcctg ctgatcctgt cgcagatcgg
     1441 ctacatgctg ggatcgggga tcctgttcgt cggcgctctc cacggccgac cgccgctgtg
     1501 gctcgtctat gggggcatag cgatgatcgc ggccgtctcg gccgtcgacg gtccgacgcg
     1561 ctcggccatg atcccgcggt tggtcggacg ggatctcctc ccggcggccc tggcgctcgg
     1621 tcaggtggtc tggaacggca ccgcgctgct cggtccggcg ctggccggga tcgtgatcgg
     1681 agccagcgag cgatacgggt tctcgatcgc gtacgggatc gacctggcca cgtacggggc
     1741 gatcctggcc gccgccttcc tcatccgtcc catgccaccg gaacgggaca ccgctgccct
     1801 gggcggcgcc gccgcggtga aggaagggtt cgcgtacctg cgcggtcggc gcgtgctgca
     1861 gtccacgttc ctgatcgacc tgatcgcgat gatcttcggc atgccgcggg cgctcttccc
     1921 gttcctggcc gcgtcccagt tccaggcggg ctcgcaggct gccgtctcgc ggatcgccgg
     1981 attcctgttc gccgccccgg ccgtgggtgc catggtggcg tcggccgcct cgggatgggt
     2041 ggggcgcgtt cggcaccagg gacgggcggt gctgatgtcc gtggcgttgt ggggtctggg
     2101 gatcgccgcg ttcgggctga tgggggatcg cctgctggcc gcgttggcgg cgctggcctt
     2161 cgccggcggt gccgatgtca tctcggccgt gttccgcaac acgatcctcc agctgtcggt
     2221 ccccgactcg ctccgcggaa ggatgtccgc gatccacatc ctggtcgtca cgggagggcc
     2281 tcggctgggg gacctggagg cgggcctggt cgcccaagcg ttcaccccca tggcgtccgt
     2341 ggtctcagga ggactgctct gtctggcggg gaccggagtc ctggccctgc tggtgcccgc
     2401 gttccgtcgg taccgcgcgg gcgaggctgc ctgaacggcg cctcaggctt tcgcggggca
     2461 ccggacgcag cgggtggccc acgggacggc ctccagccgt tccgccccga tcggcccctc
     2521 gcacacgtcg caccgcccgt aggtcccctc ctccaccttg atcagcgctc gctcgacgtc
     2581 ggtgagcttg gcgaacaggg aacgagcggc cgacgtggtg gccagccgct cgacggcttc
     2641 ggtggtccca tcgccgatcc gcttgccgaa cgacacggcc gccagcgggt cgcgcggcgc
     2701 ctcggtcagc gccgcgagct cgcggcgcag ctcggatcgg cgctcctgga gcgtctcccg
     2761 caccccgtcg acccccacgt tcagtgctcc cggacgctcc gctcggtcga ccccggcgga
     2821 cggaggatca gctggtcgtg cggctcgacc acgtcgacca cgcctcggat ccccgactcc
     2881 accagcagct cgaacccatc gccctccacc aggcgatcgc cctcgtgcgc ctcgtcggtc
     2941 agtgcgaact ccacgccgtc cccccgtcgc tgcagccgaa cggaggccct ggggttgaac
     3001 cgacgggccg ccaggtgcgt ccgccgcagg atctgcgtgg cccactcggt gaactcgatc
     3061 acgacccgat ggtaccggag ccgcgggggc tcctggctgg cctagcggcg cctgagggcc
     3121 gcgccgccga gccccgccac gccgagggcg aggtggagca gccacgccgc caccggcacc
     3181 ggaccgaaca gacccagggc ctcccccacg gcgccggccg cgacgaaccc ggtcaccccg
     3241 gcggccacca ggaacaaccc acagaccgcc aggagcaccc gagccgtccc gtccgtggcg
     3301 aagcctccca cgatcaggat gaggccgatg gcccaatggc ccgccacgac ggcgggggcc
     3361 ggcggcagcg cggcgccgag ccccgaggac catagggttc ccgcaacgcc cagagccagg
     3421 agcaccgccc cgagccaggc cgaggcggcc tgcggcgagg gcccgcggag ccgcccgccg
     3481 accgtgctcg cgcccgacgc gtcctcgtcc tcacgcaacg ggcggcgatg ctagcaggcc
     3541 gggcccggcc gggaccgggc cgatgagact cggataccct gaggcatgcc gacgatcctg
     3601 gagttcccgc tggccggcgg cggcggcgag ccggtcgacc tgcgccggac cctgaactcg
     3661 cacgggttcg tggatctccc gcccatgcgc ctgcgacccg acggatcgag cttggacatc
     3721 acgctggtcg ccggacgagc gatccggacg gtgagggtcg ggccaggcgg cggcggccgg
     3781 ggccggatca cgatcctggg tcccgcggtg ggcgcttcga ccggcaagcg gctgctggcc
     3841 tccgtgcggc acgtcctccg gctggacgag gatctctccc gcttctacga tctcgcggcc
     3901 gccgatcccg acctctcgtg ggtggcactc ggggcgggac ggatgatcca ggcgcccacc
     3961 gtcttcgagg acgtggtgaa gaccatctgc accaccaact gcagctggtc cggcaccacc
     4021 cgcatggtga cggccctcgt ggagcacctg ggcgcgcccg ccacgggcgc acccgccggc
     4081 gaagcagcgg acggggcgta tggccgggcc ttccccggcc ccgaagccat ggcggacgcg
     4141 ggcccggact tctaccggca ggtggctcgc gccggctacc ggggggcgta cctcatcggc
     4201 ctggctcgtg ccgtcgccga gggtgagctc gacctggagg ggctgcgcga ccccgccctg
     4261 cccgacgagg aggtcgagcg gcggctgctg gacctgccgg gcgtggggcc atacgcggcg
     4321 gcgcacatca tgatgacgat cgggcgttac caccggctga tcctggattc atggacccga
     4381 ccgacctacg cgaagctcgt gggacggcgg gtggtgaagg acgcggtcat ggagcgtcgg
     4441 ttccgccgct tcggcccgta cgcgggcctc gcgttctggc tgtacctcac gcgcgattgg
     4501 gtgagctgaa gcccggaacc ccttgcgcgg gagagggttg tcccggcccg gtccccgagc
     4561 gcatactgcc acgggctccg agcccggaag gttccgatcc ctgatcgaag aggaagcgat
     4621 ggccgtcccg ttatggcgag gttcccccgt cgtgcacgcc gcctgtgggc ctactggcga
     4681 gccgagcagc gcacgctccg ccagggcttc gtcgcgctct tcatcagctc gggcggcgac
     4741 ctgctcgcgg gcctcgcgct cgcattcatg accgattcgc tccagcggct cccggggctg
     4801 ttcctgctga taccagccgc catcgggatg cgcgggaaca tcttcggggc gctcggctcg
     4861 cgtctgggga cggggatcca ctccggtcag ttcgagccct ccgccgatcg ccgggggttc
     4921 ctggggcaga acgtgatggc ctccgtcttc ctcaccctgt cgacctcgtt gttcctgggg
     4981 gcggcggctc gggccgccac ggcggcgttc gggttcgcca cgatctcgat ctgggattac
     5041 ctggtgatct cggtggtggg aggggtcctc ggatcgctgg tcgtgggggg agcctcgatc
     5101 gggatcgcgg tctggtcaca ccgccgggcc tgggacctcg attccgtgtc ggcgccgttg
     5161 gtgacggcca tcggcgacat cgccacgttg cccgcgctgt gggccgcctc cttcctggtc
     5221 cagatccggt acgtcacgct cgtcctcggg atcgcggtgg cactcgtctg tttggcggcc
     5281 accgcgcggg gcatcctcta ccatctgccg ctgacgcgcc ggatcgtcat cgagtccatg
     5341 cccgtcctgg tcctcgccgg gatcgtcgac atcctggccg gcaccgtcat cgaggcacgc
     5401 ctggaacgct tcatcacatt cccggcgctg ctcgtgctgg tgccaccctt cctggaggac
     5461 accaacgcgc tgtcggggat cctctcctct cggctcgcct ccaagctcca cctcggggtg
     5521 ttggagcctc ggtccgtgcc gcagcccttg gcgctggtgg acatctcgat caacttcctg
     5581 ttcgcgatct ccgtcttcct catggtcgga gtatccgccg agctggtcag cctggcggcc
     5641 ggcaagctga gccccggcct cggcaccatg atcgggatca gcctcgccgc cggcttcctg
     5701 gccacgatcg cctcctcggc cgtcgcctac tacagcgcca tcgccacgta ccgcttcggg
     5761 ttcgatccgg acaaccacgg gatcccgatc ggctcgagcg tgatggatct ggccggcacg
     5821 ctctgcctca tcgcggctat cctgatgttc agcgtgactg cgcatggctg acgacgaacc
     5881 tcgtaacgtc aaagagctcc tggtggaggc gaaggacgcc tctgaactcc tgatcgacat
     5941 ggcgtacgcg gccgtcttct tcaacgagga ggacctggcc ggtgaggttg agaagctcga
     6001 ggagcggatg gacggctacc tccgtcgtct ccggaccctc gccatcctgg ccgcccgcag
     6061 ccccgaggac gccgaggcca tcgagccggt gctgtggatc gccgctgcca tcgacaagat
     6121 cggtgacgcc gcctcggaca tcggccgggt ggtggcagcg gatctgggga tccccgacgg
     6181 gctgcgcaag gacctccgcc acgccgacga ggtctcgggc cgggtgaagg tccgagaggg
     6241 gtcgcagtcg gtgggcgcct ccctccggga cctgtcgctg cccaccgaga ccggcatgtg
     6301 ggtcctcgcg atccggcggg gacgggagtg ggagttcgac cccggccccg acaccgtggt
     6361 ctcggaggga gacgtactcc tctatcaggg gccggaggag ggtgtgaact tgatccgaga
     6421 ggtcgccgga gcgccgccgc tcccctcggc accggagtcg gagccgcctc cgctgtccga
     6481 gctggatagg gcggtggaca tcctcgtgga gatgaagaac atctccgagg tcgcggtcgg
     6541 tctcgcgtac tcgtgcctgc tgttcaacga ccgcgccctg gcggccgagg tggcgaccct
     6601 cgaggcccga agcgacatgc tccacgacga gctcgagtcg tgggtcctgc gcgcctcgcc
     6661 cgaggcccgc aaccccgacg acctgcgcgg cctgctccgc ctgggggccg ccagcgaggc
     6721 gatctgcgat gccgctcggg acatgacctg gtacgtggag cacggggagg agctccaccc
     6781 ggtgatccag atggcgctgg aggagaccga ggagaccgcc accgagacca tcgtggacga
     6841 ggggtcccag gccgccggac gctcgctccg cgacctgcag ctcgagacgg agacgggcat
     6901 gttcgtcctg gccgtccagc gggggcgccg gtggatctac cacccgcggg gccggttcac
     6961 gctccaggcg ggcgatcgga tcatcgcggt ggggcccgag gagggcgggg aagcgctcca
     7021 ggcgctctgc cggagcaccc gggaggccgt cgagaccggc tgagccgccg gccgtggtgg
     7081 ggaccaccgc gatcgcggtg ccggacctcg aagcggctac cgggccggct gggtcccctt
     7141 cggcctggtc ctagacgcgc acgtcggaga accggagccg ttcctgggtg agccgcatct
     7201 cctccacctc ggcggccccg tgctcccgga ggaaggcgag catccgctcc accagccact
     7261 ccggagccga cgcgccggag gtgacgccca cgacgtccgc accctccagc caccaggcct
     7321 ggacctcggt ctgatcgtcc acgaggtacg ccctcgctcc ctgctgcttg gccacctcca
     7381 ccaggcggtt ggagttcgac gagttcctcg agcccaggac cagcaccacg tcggagcgcc
     7441 tggccacctc cttcaccgca tcctgccggt tctgggtggc gtagcagatg tcctcgcgcg
     7501 gcggtccctc gatggccggg aaccgatccc ggaggatctg gatgatctcg ttggtctcgt
     7561 ccaccgacag cgtggtctgg gtcaggtagc tcacgccgtc ggaggcgtcc aggggcacac
     7621 gccgggcctc ctccgggttc tgcaccagca cgatcctcgt gggagcctgg ccgaccgtgc
     7681 ccaccacctc ctcgtggccc gcgtgaccga tcaggacgat cgtgcggccg tcggcggcga
     7741 agcggcgggc ctccaggtgg accttcgtca ccagggggca cgtggcgtcc agcagcgtca
     7801 gcccccggtc gcgcgcgttc gcgtagacct ccggagcgac cccgtgggcc gacagcacgg
     7861 ccacccctcc cgccggaacg tccctctcgt cctccacgaa cacggccccc ttgccttcca
     7921 gatccctcac cacgtgcagg ttgtggacga tctgcttgcg cacgtagacg ggagggccgt
     7981 accgggccag cgcccgctcc acggcctcca ccgcccgctc cacgccggcg cagtaccccc
     8041 gtggggccgc gagcaggatc ttcctcggca cgaccctgat ggtaccgggc ggcggaccta
     8101 cggccggggc ttgacccagg gtcatatatg gagggtttgt gggtgggcgg tgtcggtgaa
     8161 cctcgatacg gtgttcggcg ttcccgacgg acgatgtccc gtccgatcct ccctccgcac
     8221 gatcgaccct tcctccagcg gtggtcggcg cgcgcggatc gtaggacgaa cggtggacgg
     8281 cgtcgtcggt agggagcgaa ggtcgaggaa gccgctcgag atgaggaggt gatccagcac
     8341 ccacgaccta ccgcgggtcg accgggtcgg tgcgcaaccg gtcgatgggg ccaggcagcc
     8401 tgccggtcca ccgcgggggt gagatccgta tggcatatct cagacatcgg cgccgcgtgc
     8461 tcctcgcggt gctcacatcg gtcgccctgc tggcgatgcc ggccacgccg gtccaggcga
     8521 gggagcgatc gcaggtaccg cggtggcggt gccggatcga ctggcagcac ggcccatggc
     8581 atgtgaagaa gctgatccga tgcgccgcca ggcactggcg ggtcccgggt ggcccggaca
     8641 aggccgtgtc ggtggcccgg tgcgagtccc ggctcgatcc gtccgcctac aacgccggcg
     8701 gcttcgcggg ggtcttccag cagtcgaccc ggtactggag gggacgggcc cggacgtacg
     8761 ggttccccgc tcactccgtg ttcaacggcc gggcgaacgt catcgtgtcg gtccggatgg
     8821 cccatcgata cggatgggga ccgtggggtt gcgcttgacc cgatcgccga cctagcggac
     8881 cgggcctccc gggcggggaa ggccctggat cgccccccgc acctcgcgca gcgaggtcac
     8941 caccacggtg gctccgggca gggcccggtc cgccacgatc cgcagggacg gataggcgct
     9001 gcgggcatcg ctcacgagct gctcggacgc cccgaagacg accaggacct gcggccgaac
     9061 ggccgcgagc tgagccatgg cgtcccgctc gtcggtggtt cccggagcca gctcccagtc
     9121 ggcggccacg gccgcccgct tcaggtcggc cagggcctga cggtcgggag ccacgcacag
     9181 cacggtcagc gcccgacaca cgctagagcc actcccgccg cttgaagacc acgtacagga
     9241 ccagggcgga taccaccatc gcccccaggg ccatcgggta gccccatgcc gagcgcagct
     9301 ccggcatgtc gcggaagttc atcccgtaga tcccggcgat cagggtgggg atcaggatga
     9361 tggccgccca ggacgtcagc tgcttcatga ccgcgttcag ccggttggag acctgggcca
     9421 gctcggactc gaatgccgag gtcaacagat cgcggatgtt gtcgatgaac tcgatcgacc
     9481 ggatcacgtg gtcctgcaca tcccggtagt acggcgccag cgccggcgtc accagcgccg
     9541 gggtctcctg gatcagatcc accacctggc gcatgggggc cgccagccgc cggaaccgca
     9601 cggcggtgcg acggagccgg aagatgtcgg cctgcagctc agcggccggc tcgtcctgga
     9661 agacccgcag ctcgatgtcg tccgagctat cctccagctt gtcgaccgca tccaggtacc
     9721 cgtcagcgat ctcgtcgagc agcaggtaca ggtggtagcc gaccccctcg tgcaccagat
     9781 cgggctcctt cgccagccgc tcgcccaccc tggccagatc gaagagcggc ggccgtcgga
     9841 cggtgatcac gtaggagccg ccgcccacga acaggtggat ctcgccatcg ctgagctggt
     9901 cgtcctcgcc cacctggagc gcatggacca cgatgaacac gtagccctga tagaactcga
     9961 ccttcgagcg ttgcccccag cggtagctgt cctccaccga gagctcgtgg agatcgaacg
    10021 tgtgctggag ctgctggagc tcctccccgg tggggtcgat cacgtccagc cataggcgat
    10081 cccctctggc gcgcgcgtcc tccacggccc cgggatcgaa cgcaccctcg tcgcggaggg
    10141 ttccgctccg gtagacgcgg cacgtgatca tgggagggag actaccccag gcgctcggac
    10201 cgatagactc cccgcgtgtc ggacctggag ccgctcctcc gcttctggcg cgccctggac
    10261 ggccggttcg gaggcgtcca tcccaccccc tggggatcgg tggtgacgga cgctcggttc
    10321 cccgcgatct gggacgtgaa ctacgcccgg ctggaggccg atcacccgat cgccctgtcg
    10381 gaggtcgagc gatcgctcct accggccctg cgccgggcgc gagctcctcg gatccacatc
    10441 gtcgccttcc atcccgaggt ccagaccgat ctggtgaccg aggcgagcac gagaggcgac
    10501 cgcctctcct gggactcgat catggtgctg cgtcggcccg tggatcgggg ttcgaccgtt
    10561 gcggtggagg agatcgccgg gttcgacgag cggttctggc gtgacgtggc gggctccatg
    10621 gccgagttcg acgtgaccga ggccgacgcc gtcgaccaga tcctccgcat cgaacgagag
    10681 atcctgggcc ccgcggggaa gcgctggttc tcggtccgct cgggccaccg cagcgtcgcc
    10741 ctggggtgcc tgctggtgct cgagggggtc gggctcgtgg accatgtggt cacccttccc
    10801 aggttccgag gacgcggata cgcctcggcg atcgtgggca ggatcgtcca ggaggcgcgg
    10861 acctcggacg tcgaagcgct gtggctcctg tgcgaaccgg acagccgagc cgaggcgctg
    10921 taccgccgcc t
//
LOCUS       DASQQQ010000292         7125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_374091, whole genome shotgun sequence.
ACCESSION   DASQQQ010000292 DASQQQ010000000
VERSION     DASQQQ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7125
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374091"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VE646_10980"
     CDS             <1..892
                     /locus_tag="VE646_10980"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZD80546.1"
                     /translation="ETAMGAGLAVIGIKLFADGVITGRTAALSRPFAEGTDTGMLIHE
                     PDALAGMVRRSIGMGLPVGIHAMGDRGIDVAIDAIEEVMGRHSSDGRPSRGPLAHPHR
                     IEHCSIPSARSLERMRSLGIVPVPQPVFLFAEGEAYLAQLGRERTAGAYPLRTMIELG
                     LRPALSSDAPATSTEDAANPWLGIAAAVTRRSWAGTELGSSERLSIEEAIALYTAGGA
                     DVLRLADRVGSVDVGREADLVVYGDDPLAMSPNDLSAIVPELVMAKGRIGHGNAAGGT
                     ASFSGRRGSPVRGLGPLPRW"
     gene            complement(968..1327)
                     /locus_tag="VE646_10985"
     CDS             complement(968..1327)
                     /locus_tag="VE646_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80547.1"
                     /translation="MSIWFQPARTNTRATVRPLRRSMVRGPEETDTGMETDIEGGTAR
                     MRSALEQERYVVAIGEAAQVLLTDRTATVLARLDGVVLVRVDRGLAERLRSLAVGTVH
                     VFANEAAARRAFGLFEH"
     gene            complement(1548..2396)
                     /locus_tag="VE646_10990"
     CDS             complement(1548..2396)
                     /locus_tag="VE646_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD80548.1"
                     /translation="MGGSYRAELLKVMKRPAMWVLAAVFLVLAQVFGYLLPYVAYRSG
                     GGTGFAAGQTAAQLLSDLLPRSLVPNTLGGFPMFAGAIAVIMGGLLAGSEYGWGTLKT
                     MLTQRPRRLSVLGGKLAVSLLMALALVVAVFAVNGLWSWVIASVEHRAAPWPGLLELG
                     RGIGAGFLIIWMWSLFGALLGILFRSTPLAVGLGMVWALVVENLVRGFAGLVGFLDAL
                     QKWLPGVNAGSLVASLGAVPQDRPGGTPGVTAAVGGPQALVALIAYVVVLALTAALVL
                     HRQDVG"
     gene            complement(2386..3327)
                     /locus_tag="VE646_10995"
     CDS             complement(2386..3327)
                     /locus_tag="VE646_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD80549.1"
                     /translation="MEAVTHVIETRDLTKVYGSRIRAVDGLDLGVRRGEVYGFLGPNG
                     AGKTTTLRMLLGLIHPTGGTAHVLGRAPGDPEGLARVGSMVEGPAFYPYLSGRDNLRV
                     VARYAGVTEGRIHAALEAVDLLGRGGDRFAAYSQGMRQRLGVAAALLKDPELLFLDEP
                     TNGLDPAGMAEMRSLIRDLGTGGRTVLLSSHLLGEVQQVCDRVGVISGGRLIAEGTVD
                     ELRGRGSILIRARPLPLARERVERLLGPERIQVQDGSLRLDADPAEGPRINRELVLAG
                     VAVRELREVERTLEDVFLELTGTNGDGRRREADTDGG"
     gene            complement(3345..3593)
                     /locus_tag="VE646_11000"
     CDS             complement(3345..3593)
                     /locus_tag="VE646_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZD80550.1"
                     /translation="MQTPLAIVGHAGWGWWWIFPLAWFALIALFWTFAWRGPWWRRRV
                     EGWHDARSPESVLGERYARGEIDEQEYRSRLAVLREHR"
     gene            complement(3729..4412)
                     /locus_tag="VE646_11005"
     CDS             complement(3729..4412)
                     /locus_tag="VE646_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD80551.1"
                     /translation="MADASSTIGVLLADDQALVRAGFRALLDAQEDIQVVGEAADGDE
                     AVRLATELRPNVVLMDIRMPGLDGLAATRAIAGDERLDDVRVVILTTFGLDEYVFEAI
                     RSGAAGFLVKDTEPAELIQAVRVVAAGDALLSPAITKRLIEEFAARTREPRPTRDLQP
                     LTDREREVMALVAAGLSNDEIAQRLFVSPATAKTHVSRAMVKLGARDRAQLVVFAYES
                     GLVAPGRGE"
     gene            complement(4436..5392)
                     /locus_tag="VE646_11010"
     CDS             complement(4436..5392)
                     /locus_tag="VE646_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015620374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZD80552.1"
                     /translation="MIRVVLADDQVLVRAGFRSLLDAEDDIEVVGEAADGETAVQLAT
                     ELAPDVVLMDIRMPGVDGLEATRRIARDRHLDRTRIVILTTFDVDEYVFEALRVGASG
                     FLVKDTEPAELVQGVRAVARGDALLSPGVTKRVIGALAERGSDPMRTAAPDPDRILAT
                     VMFSDIVSSTERAAQIGDRRWRDLLDRHDALVRRSLARHAGREVKTTGDGFLAVFDAP
                     ARAIRCALAIRHELRAEGLDVRIGLHTGEVEVRGDDIGGIAVHIGSRVAGSAAPGDVV
                     VSSTVRDLVVGAGIGFADRGEHILKGVPDRWRLYAVTDPKPT"
     gene            complement(5389..6558)
                     /locus_tag="VE646_11015"
     CDS             complement(5389..6558)
                     /locus_tag="VE646_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018532136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZD80553.1"
                     /translation="MTAITATLQAPRRRELLRDAALAAGLTLFAVAGTFGAAHGQPTR
                     RPVDGWAILLVVLAGIALAVRRRYPVAVLEFVFGTTLAFFALGYANGPIWILMVVAYV
                     TTVLRGHRLAAAITAVIGFGIFPWLDPLLRDRPDPSIAALVGLAAWLLVLYGAAELLR
                     IRRERAAEAARMREEEALRRASEERLQIARELHDALGHHLSLINVQAGVALHVNEGLP
                     DELRGSLAAIKEASKEGLTELRSVLEILRRDDERAPRSPASTLAKLDDLVAQAGAAGL
                     EVRTETAGHVRPLPFGVDVAAFRVVQEALTNVARHAGPATATVRVTYGQRELSVQVDD
                     DGRGSGSDGSGTPGTGKGVVGMRERVAALGGELEAGPRPGGGFRVRARLPLEDAR"
     gene            6772..7080
                     /locus_tag="VE646_11020"
     CDS             6772..7080
                     /locus_tag="VE646_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80554.1"
                     /translation="MEAFTSNFVSLYRDVHDRIRDEVSDLGERALGWVPGPETNSIPT
                     IVIHLLGSETEVLQVVRDLPVELFWLLNSYGHVREHLAQIQLTKRLWLQPHEMGPPTP
                     "
BASE COUNT         1039 a         2604 c         2491 g          991 t
ORIGIN      
        1 ggagaccgcg atgggcgcgg ggctcgcggt gatcgggatc aagctgttcg ccgacggcgt
       61 gatcaccggc cgcacggcgg cgctgtcgcg gccgttcgca gagggaacgg ataccggcat
      121 gctgatccac gaacccgacg cgctggccgg gatggtgcgg cggtcgatcg ggatggggct
      181 cccagtgggc atccacgcga tgggtgatcg gggcatcgac gtcgcgatcg acgcgatcga
      241 ggaggttatg gggagacaca gctcggacgg ccggccctca cgaggacccc ttgcgcatcc
      301 ccatcggatc gagcattgct cgatacccag tgccaggtcg ttggagcgca tgcggtccct
      361 cgggatcgtg ccggtgccgc aaccggtctt cctcttcgct gaaggtgagg cgtacctggc
      421 tcagctgggc cgggaacgga cggctggcgc gtaccccctc cgcacgatga tcgagctcgg
      481 ccttcgtccc gcgctgagct ccgatgcccc ggcgacctcc acggaggacg cggcgaaccc
      541 gtggctaggc atcgctgcgg cggtgacccg gaggagctgg gcggggaccg agctcgggag
      601 ctcggagcgc ctctccatcg aggaggcgat cgccctgtac acggccggtg gcgccgacgt
      661 gctccgtctc gccgatcgcg tcgggtcggt ggacgtggga cgggaggccg acctggtcgt
      721 ctacggggac gatcccctcg cgatgagccc gaacgatctg tctgccatcg tcccggagtt
      781 ggtcatggcc aaggggcgga tcggccacgg gaacgcagcc ggggggacgg cctcgttctc
      841 gggacgaaga ggatcgccgg ttcgagggtt gggtccactc cctcgatggt agggacccgc
      901 cgccgcgcga gggacgatga gcatcttgcg cggcatcggg gggttgagac ctgcgagcac
      961 ccgcggatca gtgctcgaag agcccgaacg cgcgtcgggc cgccgcctcg ttggcgaaca
     1021 cgtgcacggt gccgaccgcc aggctgcgga gccgttcggc caggccgcgg tcgacccgga
     1081 ccaggaccac gccatccagg cgcgccagca ccgtggcggt ccggtcggtg aggagcacct
     1141 gggcggcctc cccgatcgcc accacgtagc gttcctgttc cagcgcggat ctcatgcggg
     1201 cggtccctcc ttcgatgtcg gtctccatcc cggtatcggt ctcctcgggc ccgcgcacca
     1261 tggagcggcg gagcggtctt acggtcgccc gggtgttggt ccgggcgggc tggaaccaga
     1321 tgctcatcgc cgttcgacct acgactagga gggaaggggc ccatcgaccg tgccgtcctg
     1381 gtcttcgtcc tcacgctggg atggtcggtc gggatgcgct ccacggtccc cggatcatcc
     1441 ggcgcgatcg gtgaggacgc ggccgggtcc taccgcgacc ccaggtgcgg acctcgaccg
     1501 agggacatcg acagcccccg ggtcgcccgg gtcggacgcg ccctgcgcta gccgacgtcc
     1561 tgccgatgca gaacgagtgc ggcggtgagg gccagcacca ccacgtacgc gatgagggcc
     1621 accagtgcct ggggcccgcc tacggcggcc gtcacgcccg gcgtcccgcc cggccggtcc
     1681 tgcgggaccg cgcccagcga ggccacgagc gagcccgcgt tcacgccggg cagccacttc
     1741 tggagcgcgt ccaggaagcc gaccagccca gcgaaaccgc gaacgaggtt ctcgaccacg
     1801 agcgcccaga ccatcccaag gcccaccgcg agcggcgtgc tccggaacag gatgccgagg
     1861 agcgctccga acagggacca catccagatg atcaggaacc cggccccgat cccgcgaccg
     1921 agttcgagca ggccgggcca gggggccgcg cgatgctcga cggaggcgat cacccagctc
     1981 cacaggccgt tcacggcgaa cacggccacc acgagggcga gcgccatcaa gaggctcacc
     2041 gcgagcttgc cgcccagcac ggacaggcgg cgaggtcgtt gggtcagcat ggtcttcagg
     2101 gtcccccacc cgtactcgct gccggccagc aaccctccca tgatcaccgc gatggccccg
     2161 gcgaacatcg ggaagccgcc cagcgtgttc ggcaccaggc tccgcggcag cagatccgac
     2221 aggagctgtg cagcggtctg ccccgcggcg aacccggtcc cgcccccgct gcggtacgcg
     2281 acgtagggca gcaggtaccc gaacacctgg gccaggacga ggaagaccgc ggccaggacc
     2341 cacatcgcgg gccgcttcat gaccttgagc agttcggctc ggtaactacc ccccatcggt
     2401 atcagcctcc ctccgacgtc catcgccgtt cgttccggtc aactcgagga acacgtcctc
     2461 cagggtccgc tcgacctctc ggagctcgcg cacggcgacc ccggcgagca cgagctcacg
     2521 gttgatgcgc ggtccctcgg ccgggtcggc gtccagccgc agggatccgt cctgcacctg
     2581 gatgcgctcg ggtcccagca gccgttccac gcgctccctc gccaggggga gcggtcgtgc
     2641 gcggatcagg atcgaccccc gtccgcggag ctcgtccacg gttccctcgg cgatcagccg
     2701 gccgccggag atcaccccca cccggtcgca cacctgttgg acctcgccca acaggtggct
     2761 cgagagcagc acggtgcggc ccccggtgcc caggtcacgg atcagcgagc gcatctcggc
     2821 catgccggcc gggtccagcc cgttggtggg ctcatcgagg aacaggagct cggggtcctt
     2881 cagtagggcc gccgccaccc ccaaccgctg ccgcatgccc tgcgagtacg ccgcgaaccg
     2941 atccccgcct cgaccgagca ggtccaccgc ttccagcgcg gcatggatgc gaccctcggt
     3001 gacgcccgcg tagcgggcca ccacccgcag gttgtccctc ccggacaggt acgggtagaa
     3061 ggccgggccc tccaccatgg agccgacccg ggccaggccc tcgggatcgc cgggggctcg
     3121 gcccaggacg tgcgccgtgc cgccggtggg gtggatcagg ccgaggagca tccggagcgt
     3181 cgtggtcttc ccggcaccgt tgggccccag gaagccgtac acctctcccc gccggacacc
     3241 caagtctagc ccgtccacgg ccctgatccg ggagccgtag accttggtga gatcccgggt
     3301 ctcgatcacg tgtgtgacag cttccatcgg cgatcctccc ctcctcatcg gtgctcgcgg
     3361 agcaccgcca gtcgggaccg gtactcctgc tcgtcgatct ccccgcgcgc gtaccgctcg
     3421 ccgaggaccg actccggcga ccgggcgtcg tgccagccct cgacccggcg gcgccaccac
     3481 gggccgcgcc aggcgaaggt ccagaacagc gcgatcaggg cgaaccaggc gagggggaag
     3541 atccaccacc agccccagcc ggcgtgtccg acgatcgcga gtggcgtctg catctcgatc
     3601 gacctccttc cgtctcgtgt tgcctcgatc ccatgctccc ggcggaaggg tcgatcgacg
     3661 tcgcacgggg ggaggctttc gggggtacgc cgtggggtgt acgggtgggg cccgggtacg
     3721 ccgggtggct actcgcctcg accgggtgcg accagaccgg actcgtaggc gaagaccacg
     3781 agctgcgccc ggtccctcgc ccccagcttg accatggccc ggctcacgtg ggtcttggcg
     3841 gtggcggggc tcacgaacag acgctgcgcg atctcgtcgt tcgacagccc ggcggcgacc
     3901 agcgccatca cctcgcgctc acgatcggtc agcggttgca ggtcgcgggt gggccgcggc
     3961 tcgcgggtgc gggccgcgaa ctcctcgatc aggcgcttgg tgatggcggg ggagagcaga
     4021 gcgtccccgg ccgccaccac ccggacggcc tggatcagct cggccggctc ggtgtccttg
     4081 accaggaacc cggcggcgcc ggagcggatc gcctcgaaga cgtactcgtc cagcccgaac
     4141 gtggtgagga tcaccacccg gacgtcgtcc agccgctcgt ccccggcgat cgccctcgtc
     4201 gcggccaggc cgtcgagccc cggcatgcgg atgtccatga gcacgacgtt cggccggagc
     4261 tcggtcgcca ggcggaccgc ctcgtcgccg tccgccgcct cgcccacgac ctggatgtcc
     4321 tcctgcgcgt cgaggagcgc tcggaagccg gcccggacca gggcctggtc gtccgccagc
     4381 agcaccccga tcgtcgacga ggcgtcggcc atggccgggc cgttccgttc gtcgctcacg
     4441 tcggcttcgg gtcggtgacc gcgtacaggc gccaccggtc cggcaccccc ttcaggatgt
     4501 gctcaccccg gtcggcgaag ccgatgccgg cccccacgac caggtcccgg accgtgctgg
     4561 agaccacgac gtcgccgggc gcggccgagc cggccacgcg agatccgatg tgcacggcga
     4621 tgccgccgat gtcgtcgccg cggacctcga cctctccggt gtgcaggccg atccgaacgt
     4681 cgaggccctc ggctcgaagc tcgtggcgga tcgcgagggc gcagcggatg gcccgggcgg
     4741 gggcgtcgaa gacggccagg aagccgtccc ccgtggtctt cacctcgcgt ccggcgtggc
     4801 gcgccaagct ccgccggacc agggcatcgt ggcgatcgag caggtcccgc caccggcggt
     4861 ccccgatctg cgccgctcgc tcggtcgagg acacgatgtc gctgaacatc accgtggcca
     4921 ggatccggtc ggggtcgggc gcggcggttc gcatcggatc ggagccgcgc tcggcgaggg
     4981 ccccgatcac ccgcttcgtc accccgggtg acaggagggc gtcgccccgg gccacggcgc
     5041 ggacaccctg caccagctcc gccggctccg tgtccttgac caggaagccg ctggccccca
     5101 cgcgcagggc ctcgaacacg tactcgtcga cgtcgaaggt ggtcaggatg acgatccggg
     5161 tccggtccag gtgccgatcc cgggcgatcc tgcgtgtggc ctccagaccg tccaccccgg
     5221 gcatccggat gtccatgagc accacatcgg gcgcgagctc ggtcgccagt tggaccgccg
     5281 tctctccgtc ggccgcctcc cccaccacct cgatgtcgtc ctcggcgtcg agcagcgagc
     5341 ggaacccggc gcgcaccagg acctggtcat cggcgagcac gacccggatc atcgggcgtc
     5401 ctccaggggg aggcgggctc ggacccggaa cccgccgccg gggcggggcc cggcctccag
     5461 ctccccgccc agcgccgcga cccgctcgcg catgcctacc acgcccttgc ccgtccccgg
     5521 ggtgcccgaa ccgtccgagc ccgaaccgcg accgtcgtcg tccacctgga cgctgagctc
     5581 acgctgtccg taggtgacgc ggaccgtcgc cgtggccggt ccggcgtgtc gggccacgtt
     5641 cgtgagggct tcctgcacca cccggaacgc cgcgacgtcc accccgaagg gcagcggacg
     5701 gacgtgcccc gccgtctccg tccgcacctc cagcccggcg gcccccgcct gcgccaccag
     5761 gtcgtcgagc ttggcgagcg tggacgcggg ggaacggggt gcgcgctcat cgtcccggcg
     5821 caggatctcc aggaccgagc ggagctccgt gagcccctcc ttgctggcct ccttgatcgc
     5881 cgcgagcgag ccgcggagct catcggggag gccctcgttc acgtgcaggg ccaccccggc
     5941 ctggacgttg atcaacgaca ggtggtggcc gagtgcgtcg tggagctccc gagcgatctg
     6001 caggcgctcc tcgctggccc gccgcagggc ctcctcctcc cgcatcctcg cggcctccgc
     6061 ggcgcgttcg cgccggatcc ggaggagctc ggcggcaccg tagaggacca gcaaccaggc
     6121 ggccagcccg accagcgcgg cgatcgacgg atccggccgg tcccggagca gggggtccag
     6181 ccaggggaag atcccgaacc cgatgaccgc ggtgatggcg gcggccagcc ggtgaccccg
     6241 caggacggtg gtgacgtagg ccacgaccat caggatccag atcggaccgt tcgcgtagcc
     6301 gagcgcgaag aaggcgagtg tggtcccgaa gacgaactcg agtacggcca ccgggtaccg
     6361 ccgccggacg gcgagcgcga tgcccgccag gacgaccagc aggatcgccc atccgtcgac
     6421 cgggcggcgg gtgggctggc cgtgcgccgc gccgaacgtc ccggccacgg cgaacagggt
     6481 cagccccgcc gcgagggcgg cgtcgcgcag cagttcgcga cgcctcggcg cttgcagcgt
     6541 cgcagtgatc gccgtcatgg cttcgagcct aggaccatgc gtccggcccg tcatcggggc
     6601 tcgggagcga tgacgcctac cacggccggc gcaggccgag ccgatggggc tacgtcgccg
     6661 gccgtaccat catccggccg ggtcggtcga cggctcggct gggcgcgcgt ggtccctcga
     6721 gcacgatctt cgcgtgatcg agttctgacg ggaggtgtag ccgtgggata cgtggaggca
     6781 ttcacgtcga acttcgtctc tctgtaccga gacgtccacg atcggatccg agacgaggtt
     6841 tcggacctcg gcgagcgggc gctcggttgg gtgcccgggc cggagaccaa ctcgatcccc
     6901 acgatcgtca tccacctcct cggttccgag accgaggtcc tgcaggtcgt tcgcgatctt
     6961 cccgtcgagc tgttctggct gctgaacagc tacggccatg tccgggagca tcttgcccag
     7021 atccagctga cgaagcggct ttggctgcaa cctcatgaga tggggccacc caccccatga
     7081 tctcggggga ggatggcggt gtccgtgaca agctctacga tcgga
//
LOCUS       DASQQQ010000293         6881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_190843, whole genome shotgun sequence.
ACCESSION   DASQQQ010000293 DASQQQ010000000
VERSION     DASQQQ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190843"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VE646_11025"
     CDS             <1..278
                     /locus_tag="VE646_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80555.1"
                     /translation="AVPAFTLAMVHGVFAGTDTARPWMWWTYLATGLIALFLVLVRGL
                     TAGERPQRAPRPAHAPARPAPTPARRAPQHEASRTPPVAPVEVRVEG"
     gene            275..3016
                     /locus_tag="VE646_11030"
     CDS             275..3016
                     /locus_tag="VE646_11030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZD80556.1"
                     /translation="MSADRMVDVGEYPAYLASDVVLRDGSTVRIRPALKSDATRVEDY
                     LLGLSPETRRLRFWGSAIDVRSVAEKIVDVDYHDHLTLLVLSGGDEGRMIGGAQFTRI
                     EGGRAEIGLSVTDEFQGKGIGSILLGQLAQAAAEQDITIFIADVLPENHNMVNVFRHS
                     GFPVSIRATPGSIDVEFPISLTEEAVEHFERRETAAAVSAMQTFLTPDSVAVIGASRD
                     TSSIGGMLFHNLLTKEFHGPVYPVNPKAAVVHGVPAYPSILDVPGPVEVAFIAIPAAF
                     VSAAAEECGRKGVRGLVVISSGFGEVGGEGPARQEELIDICRRYGMRMIGPNCMGVVN
                     TSETIKLDGTFATVYPPPGRVGFLSQSGALGLAVMQYASDLGLGLSTFVSVGNKADIS
                     SSDLIAYWNEDLGTDVVLLYMESVGNPRRFARLAREVGRRKPIVAVKSGRSAAGARAA
                     ASHTGALLAASDVTVDALFRQAGVIRTDTLEEMFDVATILANQPPPKGHRIAIVTNAG
                     GLGILCADTCEANGLTVQPISERTKEELRSFLPAEAAVGNPVDMIASATDEDYRRTIR
                     TVAEDPEVDAMIVIFIPPQATKADEIAHGIARGIGDARGRIPIATTFMSAKGLPEELR
                     SNGTRIPSFAFPEQAAIAMAHACAYGRWREKPEGTVPRFHDIRASEAAAIVAGALQRE
                     ESGGWLSAEEVDRLLACFGLPMVRSERTTTPDEAGQAAARIGTPVALKAFGPDIVHKT
                     DVGAVRLGLSGSVAVREAAIQMRERIGEAGLNLEGFLIQEMIRDGVEMLVGVAHDPVF
                     GPVVACSAGGTAVELVRDVSVSVTPISDLDAAEMVRSLKTFPLLNGFRGAPKADVAAL
                     EDVILRVSTLVDTLPEIAEMDCNPVMVLPHGASIVDVRIRVQEAPQLKPLAARAAAG"
     gene            complement(3041..3892)
                     /locus_tag="VE646_11035"
     CDS             complement(3041..3892)
                     /locus_tag="VE646_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZD80557.1"
                     /translation="MSSGGPRAPLSRSLAGTDGSERAQEAVRQSARLAALTGASLDVV
                     FVIDARHPHEDRDVEAVAEEVLGRAEEVARREQVEPTTRVLAGDPAEVLVAEAAQIGA
                     ELICLGPDAGAFGGTIRVGRVAAHVIRHAGCSVMLARGSPQGFPRRIQCGVDGSDSSV
                     ETARLAAGIAAVAGAELHLQHVIPIFRGDNQEWSLDRDEPTPPELIPAVDAARSWGVE
                     PVRDMAMGRPENALVETARRDQADLTVVGSRGLSGMARVLLGSVCEYVAQHAPCSVLI
                     ARPGPTG"
     gene            complement(3889..4596)
                     /locus_tag="VE646_11040"
     CDS             complement(3889..4596)
                     /locus_tag="VE646_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019072592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZD80558.1"
                     /translation="MKRTVKDVMTRTVVVVSDSSPFKDVVRRIQEYRVSAVPVVDQQD
                     RLVGIVSEGDLILKEDAQLEGDPRLLEGRHRRIKREKHAGLVAGQLMTTPVITVEPDA
                     SLGDAARLMYRHGVKRLPVVGARGDVLGIVSRADLLKVFLRDDAEIAAEIREDVIRRT
                     LWIEPSSIRVMVRDGVVRLEGQIERRSLIPVVVGLVQAVEGVVGVENHLSHLEDDTLP
                     SPELPLPWTAITPGPGR"
     gene            4741..6357
                     /locus_tag="VE646_11045"
     CDS             4741..6357
                     /locus_tag="VE646_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HZD80559.1"
                     /translation="MDHRIDRRDRLIEAGLTLSSELSLEGVLQRIVELAVEVTGARYG
                     ALGVIGAEGRLIEFLTTGVSDAERERIGHPPEGHGILGALIREGGALRIPDIAADPRS
                     VGFPPNHPPMRSFLGMPIRSRDEIFGNIYLTEKQGAPGFTPEDERDLSVLAAQAGVAV
                     ENARLYREAQRRRRWLEAVREIATSILSGAPPEQALEIIADRALELVGAEIATVAVPE
                     ASTGRLTLRVARGVGADTLRGMSFPIKGSISGEVVAKGEAIAVEDASSDPRAYQPVVA
                     VGGFGPSIFVPLMAHGSAFGTLMAANQVGGRSFEPGDAGLLQTFADQASLALEYGRAQ
                     QENQRLMVLEDRERIAKELHDGVIQALFAVGMGLQGTALMSQDEELAERIESAVGELD
                     RVIRDLRNYIFGLRPGILADRQLDQALRALGEEFQQKSGVVTVVEVDSTVASELSSHA
                     SDLLQLARESLSNVGRHAEASTCRLSLYRNGQGAILEVDDDGRGFDPATVRRGDGLSN
                     MEERIDTLGGELSIDSSPAEGTTVRLFLPF"
     gene            complement(6354..6779)
                     /locus_tag="VE646_11050"
     CDS             complement(6354..6779)
                     /locus_tag="VE646_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80560.1"
                     /translation="MAAQAGGGAVLVVEADAAERERLGSWLEAAGHQVLTCPGPTEPD
                     YTCVGSRGRTCPLLEDASAVILDMSLASEAVGSGTSAEDLLGLYLLSGRRIVALGSRP
                     GPDVPGQLLRIGRHPTRRGLLDALDRILGTEGRPGSDRP"
BASE COUNT          962 a         2465 c         2507 g          947 t
ORIGIN      
        1 tggccgtgcc ggcgttcacg ctggccatgg tccacggggt cttcgcgggg acggacaccg
       61 cccggccctg gatgtggtgg acctacctgg cgaccggcct gatagcgctg ttcctggtgc
      121 tggtgcgcgg gctcacggcc ggcgaacggc cccagcgcgc cccgcgcccc gcccatgccc
      181 cggcgcgccc ggcccccacg ccggcccgac gggccccaca gcatgaggcc tcccggaccc
      241 ctccggtcgc tccggtggag gtcagggttg aggggtgagc gccgatagga tggtggacgt
      301 gggcgagtac cccgcgtacc tcgcgtccga cgtggtcctg cgcgacggat ccaccgtgcg
      361 gatccgcccc gccctgaaga gcgatgccac ccgggtcgag gactacctcc tcggactgtc
      421 gcccgagacc cggcggttgc gcttctgggg cagcgcgatc gacgtccgca gcgtcgccga
      481 gaagatcgtg gacgtcgact accacgatca cctgaccctg ctggtcctct ccggcggcga
      541 cgagggccgg atgatcggcg gcgcccagtt cacccgcatc gagggcggac gggccgagat
      601 cggcctgtcg gtcaccgacg agttccaggg caagggcatc gggtcgatcc tgctgggcca
      661 gctggcccag gcggccgccg agcaggacat cacgatcttc atcgccgacg tcctgcccga
      721 gaaccacaac atggtgaacg tgttccggca cagcggcttc cccgtgtcga tccgggcgac
      781 ccccggcagc atcgacgtgg agttcccgat ctcgctcacg gaggaggccg tggagcactt
      841 cgagcggcgg gagacggccg ccgccgtgag cgccatgcag acctttctga cccccgattc
      901 ggtggcggtg atcggcgcgt ccagggacac ctcgagcatc gggggcatgc tcttccacaa
      961 cctgctgacc aaggagttcc acggccccgt ctacccggtg aaccccaagg ccgccgtggt
     1021 ccacggcgtg cccgcctacc cctcgatcct ggacgtgccg ggcccggtcg aggtggcgtt
     1081 catcgcgatc cccgcggcct tcgtgtccgc cgcggccgag gagtgcggca ggaagggcgt
     1141 ccgcgggctg gtcgtgatct cctccggctt cggcgaggtg gggggtgagg gccccgcgcg
     1201 gcaggaggag ctgatcgaca tctgccgccg gtacgggatg cggatgatcg ggccgaactg
     1261 catgggcgtc gtcaacacct cggagacgat caagctcgac ggcacgttcg ccacggtcta
     1321 tccaccgccg ggccgggtgg ggttcctgtc gcagtccggc gcgctggggc tggccgtgat
     1381 gcagtacgcg tcggatctgg ggctgggtct gtccacgttc gtctccgtgg ggaacaaggc
     1441 cgacatctcc agcagcgacc tgatcgcgta ctggaacgag gacctgggga ccgacgtggt
     1501 gctgctgtac atggagtcgg tggggaaccc ccgacggttc gcccgcctgg cccgagaggt
     1561 gggacggcgc aagccgatcg tggcggtgaa gtcggggcgc agcgccgccg gggcgcgcgc
     1621 ggcggcctcg cataccggtg ccctgctggc cgcctccgac gtcacggtgg acgccctgtt
     1681 ccggcaggcc ggcgtgatcc gcacggacac gttggaggag atgttcgacg tggccacgat
     1741 cctggcgaac cagccgcccc ccaagggcca ccggatcgcg atcgtcacca atgccggggg
     1801 cctggggatc ctgtgcgccg acacctgcga ggccaacggc ctgacggtgc agccgatctc
     1861 ggaacgcacg aaggaggagc tgcgctcgtt cctgccggcc gaggcggcgg tgggcaaccc
     1921 ggtggacatg atcgcctccg ccaccgacga ggactaccgg cggacgatcc gcacggtggc
     1981 cgaggaccct gaggtcgacg cgatgatcgt gatcttcatc ccaccgcagg ccaccaaggc
     2041 cgacgagatc gcgcacggga tcgcccgggg catcggcgac gcgcggggcc ggatccccat
     2101 cgccaccacg ttcatgtcgg ccaagggcct gcccgaggag ctgcgcagca acggcacccg
     2161 gatcccctcg ttcgccttcc ccgagcaggc cgccatcgcc atggcgcacg cctgcgccta
     2221 cgggcgttgg cgcgagaagc cggagggcac cgtcccccgg ttccacgaca tccgggcctc
     2281 cgaggccgcg gcgatcgtgg ccggcgcgct gcagcgcgag gagtccggcg gctggctctc
     2341 ggcggaggag gtggaccggc tcctggcctg cttcgggctg ccgatggtgc gctcggagcg
     2401 gacgaccacg cccgacgagg ccgggcaggc cgcggcccgg atcgggaccc cggtcgccct
     2461 caaggcgttc gggccggaca tcgtgcacaa gaccgacgtg ggcgcggtcc ggctcgggtt
     2521 gagcggctcg gtggcggtcc gggaggccgc gatccagatg cgcgagcgga tcggggaggc
     2581 cggcctgaac ctggagggct tcctgatcca ggagatgatc cgggacgggg tggagatgct
     2641 cgtgggtgtg gcccacgacc cggtgttcgg gccggtggtg gcctgcagcg cgggaggcac
     2701 ggccgtggag ctggtgcggg acgtctcggt gagcgtcacg ccgatcagcg acctggacgc
     2761 cgccgagatg gtccgctccc tcaagacctt ccccctgctg aacggcttcc ggggcgcgcc
     2821 gaaggccgac gtggccgcgc tcgaggacgt gatcctgcgg gtgagcaccc tggtggacac
     2881 cctcccggag atcgccgaga tggactgcaa cccggtgatg gtgctccccc acggcgcgtc
     2941 catcgtggac gtccggatcc gggtgcagga ggctccgcag ctgaagccgc tcgcggcccg
     3001 ggccgccgcg ggatgagcgc cgtcggaggg ataggacggc ctagcccgtc gggccggggc
     3061 gcgcgatcag caccgagcat ggcgcgtgct gcgccacgta ctcgcacacg ctgccgagga
     3121 gcacccgggc catccccgac aggccccggg acccgaccac ggtgaggtcc gcctggtccc
     3181 ggcgggccgt ctccacgagc gcgttctccg gccgccccat cgccatgtct cggaccggct
     3241 ccacgcccca ggatcgggcg gcgtccacgg ccgggatcag ctcgggcggt gtgggctcgt
     3301 cccggtccag gctccactcc tggttgtcgc cccggaagat cgggatcacg tgctggaggt
     3361 ggagctcggc gccggcaacg gccgcgatgc cggccgccag ccgtgcggtc tccacggagc
     3421 tgtccgaccc gtccaccccg cactggatcc gacgggggaa cccctggggg ctccctcgcg
     3481 cgagcatgac cgagcagccc gcgtgccgga tcacgtgcgc cgccacccgg cccacccgga
     3541 tcgtcccgcc gaacgcgccg gcgtcgggtc ccaggcagat cagctcggcg ccgatctgag
     3601 ccgcctcggc caccaggacc tcggccgggt ccccggccag taccctggtg gtcggctcca
     3661 cctgctctcg ccgggccacc tcctcggccc ggccgaggac ctcctcggcc acggcctcga
     3721 cgtcccggtc ctcgtgcggg tgcctcgcgt cgatcacgaa gacgacgtcc agcgacgctc
     3781 cggtcagggc ggccagacgg gcggactgac ggacggcctc ctgcgcgcgc tccgagccgt
     3841 cggtgcctgc cagcgatcga gacagggggg ctcggggtcc gcccgagctc accgtcccgg
     3901 tcccggggtg atcgcggtcc agggcagggg cagctcgggc gagggcaacg tgtcgtcctc
     3961 caggtgcgag aggtggttct ccacgcccac gaccccctcc accgcctgca ccaggcccac
     4021 cacgaccggg atcaggctcc gccgctcgat ctgcccctcc agacggacca cgccgtcccg
     4081 caccatgacc cggatgctac tcggttcgat ccacagggtg cggcggatca cgtcctcccg
     4141 gatctcggcg gcgatctcgg cgtcgtcgcg caggaacacc ttcagcaggt ccgcacggga
     4201 gacgatcccc aggacatcgc cccgggcccc gaccaccggc aagcgcttca ccccgtgccg
     4261 gtacatcagc cgcgccgcgt cacccaggga ggcgtcgggc tccacggtga tgaccggcgt
     4321 ggtcatcagc tggcccgcca ccagcccggc gtgcttctcc cgcttgatcc gccgatgccg
     4381 gccctcgagc aggcgggggt cgccctcgag ctgagcatcc tccttcaaga tcaggtcgcc
     4441 ctccgagacg atgccgacca gccggtcctg ctggtccacg acggggaccg cgctgacccg
     4501 gtactcctgg atgcggcgga cgacatcctt gaacggggac gagtcgctca cgacgacgac
     4561 cgtgcgggtc atcacgtcct tcaccgtccg cttcatgctc gcctcctccc aggatgcccc
     4621 cacccacggt acgggcggtt ccgggggttc catagggtcg gagggcccgg gccgccgggt
     4681 cccaggaccc gctcgcacgg gggccgggcg accccgttgc acggtacgct aacggtgggg
     4741 atggaccacc ggatcgaccg cagggaccgg ctgatcgagg ccgggctgac gctctcttcc
     4801 gagctctcgc tggagggcgt gctgcagcgc atcgtggagc tggccgtgga ggtcacgggc
     4861 gcccgttacg gagccctggg ggtgatcggt gcggagggcc gcctgatcga gttcctcacc
     4921 accggggtct ccgacgccga gcgcgaacgc atcggccacc caccggaggg ccatgggatc
     4981 ctcggagccc tcatccgtga gggcggggcc ctgcgcatcc cggacatcgc cgccgatccc
     5041 cgctcggtgg ggttcccccc gaaccatccc ccgatgcgct cgttcctggg gatgccgatc
     5101 cgatcccggg acgagatctt cggcaacatc tacctcaccg agaagcaggg cgccccgggg
     5161 ttcaccccgg aggacgagcg ggatctctcg gtcctggccg cccaggccgg cgtggcggtg
     5221 gagaacgccc gcctgtaccg ggaggcgcag cggcggcgcc ggtggctcga ggcggtccgc
     5281 gagatcgcca cctcgatcct gtcgggcgcc ccgccggagc aggccctcga gatcatcgcg
     5341 gatcgggcgc tcgagctggt cggggcggag atcgccaccg tggccgtgcc cgaggccagc
     5401 accggacggc tcacgctgcg cgtggcccga ggggtgggag ccgacacgtt gcggggcatg
     5461 agcttcccga tcaagggctc gatctcgggc gaggtggtgg ccaagggaga ggccatcgcg
     5521 gtggaggatg catcgagcga cccccgcgcc taccagccgg tggtggcggt gggcgggttc
     5581 gggccctcca tcttcgtgcc gttgatggcg cacgggagcg cgttcgggac cctgatggcc
     5641 gcgaaccagg tgggcggccg ttccttcgag ccgggcgacg cgggcctgct ccagaccttc
     5701 gccgaccagg cctcgctggc gctggagtac ggtcgggccc agcaggagaa ccagcggctg
     5761 atggtcctgg aggatcggga gcggatcgcc aaggaactgc acgacggcgt gatccaggcc
     5821 ctgttcgcgg tgggcatggg ccttcagggc accgccctga tgagccagga cgaggagctc
     5881 gccgagcgca tcgagagcgc cgtgggcgag ctggaccggg tgatccgcga cctgcgcaac
     5941 tacatcttcg gtctgcgccc ggggatcctg gccgaccgcc agctcgatca agccttgcgg
     6001 gccttggggg aggagttcca gcagaagagc ggggtggtca ccgtggtgga ggtggacagc
     6061 accgtggcct ccgagctcag ctcgcacgcg agcgacctgc tccagttggc ccgggaatcg
     6121 ctgtccaacg tgggccggca cgccgaggcc tccacctgcc ggctctcgct gtaccgcaac
     6181 ggccagggag cgatcctgga ggtggacgac gacggccgtg ggttcgatcc ggccacggtg
     6241 cggcggggcg acgggctgtc gaacatggag gagcggatcg acacgctggg cggcgagctg
     6301 tcgatcgaca gctcgcccgc cgagggcacc acggtgcggc tgttcctgcc gttctagggc
     6361 cgatccgaac cgggtcgacc ttcggtcccc aggatccggt cgagcgcgtc caggagcccc
     6421 cgacgcgtgg gatgacgacc gatccggagc agctgtccgg gcacatccgg tccggggcgg
     6481 gagcccagcg cgacgatccg ccggccgctc agcaggtaga gccccaggag gtcctcggcg
     6541 gaggtgccgc tgccgacggc ctcgctggcc aggctcatat ccaggatcac ggccgaggcg
     6601 tcctccagca gcggacaggt gcggccacga gacccgacac atgtgtagtc gggctcggtg
     6661 gggccggggc aggtcaggac ctgatggccg gcggcctcga gccaggagcc cagccgctcc
     6721 cgctccgccg cgtcggcctc cacgaccagc acggcgcccc ctccggcctg cgccgccatg
     6781 cgccgaagtc tgagagcggt cccccggggc ggtgccgtgc cggaggtccc gccgagggga
     6841 ggacctccga ccctcgggag cgccccgggg gactcgcatc c
//
LOCUS       DASQQQ010000294         4926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_297239, whole genome shotgun sequence.
ACCESSION   DASQQQ010000294 DASQQQ010000000
VERSION     DASQQQ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297239"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..771)
                     /locus_tag="VE646_11055"
     CDS             complement(58..771)
                     /locus_tag="VE646_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HZD80561.1"
                     /translation="MTEQLCATDAYLRTCEATVLSVDETGVVLDRTVFYARGGGQPGD
                     TGVFRWEDGEVPVTDTRKVGGDVVHQVDGRPPPPGTTVTAQIDWERRHALMRTHTALH
                     ALSGIVWRDYGAKVTGGNMEPGVARMDFELEAISQEFGQEVERRLNAALGKDRPVRVG
                     FLARAEALRDPDLIRTKVNLIPEQVDPIRVIDIEGLDKQADGGTHVRSTAEVGPVRVV
                     KTESKGKGFKRMRIELVQD"
     gene            complement(827..1354)
                     /locus_tag="VE646_11060"
     CDS             complement(827..1354)
                     /locus_tag="VE646_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006628535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HZD80562.1"
                     /translation="MSELEEFRRGKDAFFREGPTSPLTDEQRRSFEGLAYYPPTDALV
                     IRAPLDPDVDRQEPVVMQTTTGGSQEYRRAGVVRFRVDGDVAQVTLYASPDQRELFLP
                     FRDATSGKESYGAGRYLEVEPPAPDGTVEIDFNYAYNPYCAYNEAWSCPLPPGENWLR
                     VPIRAGERTFPGHRP"
     gene            complement(1351..2136)
                     /locus_tag="VE646_11065"
     CDS             complement(1351..2136)
                     /locus_tag="VE646_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZD80563.1"
                     /translation="MAEVVTGDVTLHVEVDGEGDPVTVVAHGLTNNCKELAALTPLVP
                     GSAVRFDFRGHGSSGAPETGYRFRDFADDLEAVADAYGATCAVGTSLGAGAIANLLCR
                     KPDRFERMVWLLPAGLDGPFAYKDRYRALASELDGKSPDEAIEAIISDPERVAEYVQA
                     PWRAELDKVMWAHEHPEGVARAIRGVIEDWPVPDRQLLRRVTAPTILVCVEGDEIHPV
                     ELGHILHDLLPNSELLVFSSQDELFSEIPTIVQKVAVFLASAA"
     gene            complement(2210..>4926)
                     /locus_tag="VE646_11070"
     CDS             complement(2210..>4926)
                     /locus_tag="VE646_11070"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD80564.1"
                     /translation="HFVTARFSLGSALKRDAPSLMSIGFGMVLGLGSWLVGPWGVTGS
                     LADAALPPPYTQAVFEKRLWQWVLSLQPVITFPLILLAIGWVIYRARRDRRPASGFGT
                     LSALWLLPLVGIFGFLLGKAYPYYRFMNATAAIMPLVGLGAWVAARWLLRRQGGRRIL
                     GALGVAALVGALAFVWVRGRDSSQWANPNNQWIDQPTRTALAAASAIVQREPGRPIVF
                     ILNFGDTYQSYGWSKTFTNVSRTGLPGDAVKRSMSYFGSVEDFLADRPTQLTDGTYNK
                     MSRGFFRDLQGLERAYPQPPVAFLVRQFNGKTQNTALLDQQPVPDFLVKISDDLAVVT
                     GTDLTTPSPAALDAARGAERRTAALYADHPGPLGNLGHTGRVLAALALLLVVPGLISA
                     RYFELEDVWTKVALVPGISIALTVIAGIVVVAVHRAPFGVADGWATLGLAVVAAVALR
                     LAKDPLDRAFGALSRFFGEMFAVFSNRSFSALMATQFVAQAADGIVQASLAKSIAFGG
                     QKGFDVTSAPSARYLLAIVLALYIPYTIVSPFVGAFIDRYDRRQLLSRSNLFRAAVVA
                     LAALALAAGGDGLPDVVLIVAVLTTLACTRILLAIKSAGLPAVVHGKDLLQANGLSQA
                     GGAIFQVLGGGVALVGTALAPAWIVAVLGAGLYVVAAVVANRVERLEYERHVARFLEE
                     ARRVLGDIASGVREVFGRPAAALGLSGFQALRMEFFGFVALAFALEARHLLSGANSDK
                     TVVAIAGAFGAIGAAIGMVMAQKLKDRVAPYRLLVVAMAAVGAGVIVFGGVHSIVAYS
                     ALMFIGALGFFIGKIAADTIMQQALPDDFRGRGFSLFDIAYNLGWIVPAFVLALVWSA
                     GRVRPILVTSGVVFLVATAAIYAWALRIKGELASQDDVDDVASPTAPV"
BASE COUNT          825 a         1774 c         1673 g          654 t
ORIGIN      
        1 gaagtatcgc aggacagcgg tgggagaccc gaggggacgt gccgaggtcc gggcgggtca
       61 gtcctgcacg agctcgatgc gcatgcgctt gaagcccttg cccttgctct cggtcttgac
      121 cacccgaacc gggcccacct cggccgtgga ccggacgtgg gtgccgccgt ccgcctgctt
      181 gtcgagcccc tcgatgtcga tcacccggat cgggtccacc tgctcgggga tcaggttcac
      241 cttggtccgg atcagatcgg gatccctcag cgcctccgcc cgagccagga acccgacccg
      301 gaccgggcgg tccttcccga gcgcggcgtt cagcctccgc tcgacctcct ggccgaactc
      361 ctggctgatc gcctccaact cgaagtccat ccgcgcgacc ccgggctcca tgttccctcc
      421 ggtgaccttc gccccgtagt cgcgccagac gatgccggag agggcatgga gggcggtgtg
      481 cgtgcgcatc agcgcgtggc gccgctccca gtcgatctgg gcggtgacgg tggtgcccgg
      541 cggcggcggc cgaccgtcga cctggtggac cacgtcaccg ccgaccttcc gggtgtccgt
      601 taccggcacc tcaccgtcct cccaacggaa gacaccggtg tccccaggtt gcccccctcc
      661 cctggcgtag aagacggttc tgtccagcac cacgccggtc tcatccaccg agagcacggt
      721 ggcctcgcac gtacgcaggt aggcatcggt ggcgcacagc tgctcggtca cggggcgagg
      781 gtacacgagg cccgagcgaa gcggcgggat cgtggagcgg gaaggatcag ggtcggtgac
      841 cggggaacgt gcgctcgccg gcccggatgg gcacccggag ccagttctcg cccggcggga
      901 gcggacagct ccacgcctcg ttgtaggcgc agtaggggtt gtaggcgtag ttgaagtcga
      961 tctccaccgt gccgtccggg gccgggggct cgacctccag gtaccgcccg gcgccgtagc
     1021 tctccttgcc cgacgtggcg tcccggaagg gcaggaacag ctcgcgttga tcgggcgagg
     1081 cgtagagcgt gacctgggcg acatccccat ccacccggaa ccgaaccaca ccggctcgcc
     1141 ggtactcctg ggagccgccc gtggtggtct gcatcacgac gggctcctgt cggtcgacat
     1201 ccgggtcgag gggtgcccgg atcaccaggg catcggtcgg cgggtagtag gcgagcccct
     1261 cgaacgaacg gcgctgctcg tcggtcaacg gcgaagtcgg accctcgcgg aagaacgcgt
     1321 ccttgccgcg acggaactcc tccagctcgc tcacgccgcg gatgccagga agacggcgac
     1381 cttctgcacg atggtcggga tctcggagaa cagctcgtcc tggctgctga acaccaggag
     1441 ctcggagttc gggagcaggt cgtgcaggat gtggccgagc tcgacgggat ggatctcgtc
     1501 cccctcgacg cagaccagga tggtcggggc cgtgacccgt cgcagcagct ggcggtccgg
     1561 taccggccag tcttcgatca cgccgcggat cgcccgcgcc acgccctcgg ggtgctcgtg
     1621 cgcccacatc accttgtcga gctcggccct ccagggcgcc tggacgtact cggccacgcg
     1681 ctcggggtcc gagatgatcg cctcgatcgc ctcgtcgggg ctcttgccgt cgagctcgct
     1741 cgccagggcc cggtaccgat ccttgtacgc gaagggaccg tccagccccg ccgggagcag
     1801 ccagaccatc cgctcgaacc ggtcgggctt gcggcagagg agattggcga tggcaccggc
     1861 accgagggac gtgcccaccg cacaggtcgc cccgtaggcg tctgccacgg cctccaggtc
     1921 atcggcgaag tcccggaacc gatagccggt ctccggggct ccggacgagc cgtgcccgcg
     1981 gaagtcgaat cggaccgccg agccgggaac cagcggagtg agggcggcca gctccttgca
     2041 gttgttcgtc aggccgtggg cgacgaccgt caccgggtca ccctccccat cgacctccac
     2101 gtgtagcgtg acgtccccgg tcacgacctc agccacgtcg cacctccgcg ggctccatac
     2161 cccatcagcg tacgggcgac accgtcgaac gacgggccgg ccgccatcgt cacacgggag
     2221 cagtgggcga ggcgacgtcg tccacgtcgt cctgcgaggc aagctcgccc ttgatccgga
     2281 gtgcccatgc gtagatcgcg gccgtcgcca ccaggaacac cacgcccgat gtcaccagga
     2341 tcggccgcac ccggcccgcg gaccacacca gcgcgagcac gaacgccggc acgatccagc
     2401 ccaggttgta cgcgatgtcg aagaggctga accctcgtcc gcggaagtcg tcgggcagcg
     2461 cctgctgcat gatcgtgtct gccgcgatct tgccgatgaa gaacccgagc gccccgatga
     2521 acatcagcgc gctgtaggcg acgatcgagt gcacgccccc gaacacgatg acgccggcgc
     2581 cgaccgcggc catcgcgacc accagcagtc gatagggggc gacccggtcc ttcagcttct
     2641 gcgccatgac catcccgatg gcggccccga tcgccccgaa cgctccggcg atcgccacca
     2701 ccgtcttgtc ggagttggcg ccggagagca ggtggcgagc ctccagggcg aaggccaggg
     2761 ccacgaaccc gaagaactcc atccggagcg cctggaaacc cgacagaccg agcgccgccg
     2821 ccggccggcc gaacacctcc cgtaccccgc tcgcgatgtc cccgagcacc cgcctggcct
     2881 cctcgaggaa gcgggccacg tgccgttcgt actcgagccg ctcgacccgg ttggccacca
     2941 cggcggccac cacgtagagc cccgctccca gcacggccac gatccaggcc ggcgccaggg
     3001 cggtacccac caacgccaca ccgcccccga gcacctggaa gatcgcgccg ccggcctggg
     3061 agagcccgtt cgcttgcagg aggtccttgc cgtgcaccac ggcagggagc ccggcggact
     3121 tgatggcgag caggatccgg gtgcaggcca gcgtggtcag gaccgccacg atcaggacca
     3181 catcgggcag accgtcccct ccggccgcca acgcgagcgc cgccagggcc accaccgcgg
     3241 cccggaacag gttcgaacgg gacaacagct gacgccggtc gtatcgatcg atgaacgcac
     3301 cgacgaaggg gctcacgatc gtgtacggga tgtagagcgc caggacgatc gccagcaggt
     3361 accgggctga cggcgcgctg gtcacgtcga accccttctg cccgccgaag gcgatcgact
     3421 tggccaggga cgcttgaacg atgccgtcgg cggcctgggc gacgaactgg gtggccatca
     3481 gagcggagaa gctgcggttg gagaagacgg cgaacatctc cccgaagaac cgagacagcg
     3541 cgccgaaggc acggtcgagc gggtccttgg ccaaccgcag cgccacggcc gccaccaccg
     3601 cgagccccag ggtggcccac ccgtcggcca caccgaacgg cgcacggtgg acggcgacca
     3661 ccacgatccc ggcgatcacc gtgagagcga tggagatccc ggggacgagg gccaccttgg
     3721 tccagacgtc ctccaactcg aagtaccgag ccgagatgag ccccgggacc accaacagca
     3781 gggccagggc cgcgagcacc cggccggtat ggccgaggtt cccgagcggc cccggatggt
     3841 cggcgtacag ggctgccgtc cgccgctcgg ccccccgagc cgcgtccagg gcagcggggg
     3901 agggcgtggt caggtccgtc ccggtgacca ccgccaggtc gtcgctgatc ttcaccagga
     3961 agtccggcac gggctgctgg tccagcaggg ccgtgttctg cgtcttcccg ttgaactgac
     4021 ggaccaggaa cgcgaccggc ggttgcgggt acgcgcgctc gagcccctgc aggtcgcgga
     4081 agaacccgcg cgacatcttg ttgtaggtgc catccgtcag ctgggtgggc cggtccgcga
     4141 ggaagtcctc cacgctgccg aagtaggaca tggaccgctt cacggcatcg ccgggcaggc
     4201 cggtccggga gacgttcgtg aaggtcttcg accagccgta ggactggtac gtgtccccga
     4261 agttcaggat gaacacgatg ggtcgccccg gctcgcgctg gacgatggcg ctggccgcgg
     4321 ccaaggcggt ccgggtgggc tgatcgatcc actggttgtt cgggttcgcc cactgggagc
     4381 tgtcgcggcc gcgcacccat acgaaggcga gcgcgcctac gagcgccgcc accccgagcg
     4441 cgcccaggat cctccgacca ccttgccgtc ggaggagcca gcgggccgcc acccacgccc
     4501 ccagcccgac cagcggcatg atcgccgcgg tggcgttcat gaaccggtag tacgggtagg
     4561 ccttgcccag caggaagccg aagatcccga ccagcgggag gagccacagg gccgagagcg
     4621 tgccgaaccc cgaggcgggg cgccgatcac gccgggctcg gtagatcacc cagccgatcg
     4681 ccagcaggat gagcgggaac gtgatcaccg gctgcagcga gagcacccac tgccacagac
     4741 gcttctcgaa caccgcctgc gtgtagggag ggggcagggc cgcatccgcc aacgaccccg
     4801 tcacgcccca ggggcccacc agccacgacc ccaggcccag caccatcccg aacccgatcg
     4861 acatcaggct cggcgcgtcg cgcttgagcg ccgaccccag ggagaagcgc gcggtgacga
     4921 agtgca
//
LOCUS       DASQQQ010000295        12974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_80712, whole genome shotgun sequence.
ACCESSION   DASQQQ010000295 DASQQQ010000000
VERSION     DASQQQ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12974
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80712"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..976
                     /gene="aroF"
                     /locus_tag="VE646_11075"
     CDS             8..976
                     /gene="aroF"
                     /locus_tag="VE646_11075"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003086669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZD80565.1"
                     /translation="MAERVREVGGEAFVSRGAVHTIIGLVGDTATFRSLDLDNLPGVD
                     HLIQIGKPYKMVARALHPQPSTVKVGPAAVGRELFALIAGPCAVESEEQALAAARAAK
                     AAGATILRGGAFKPRTSPYSFQGLGRQGLEILAGCRQETGLPVVTEVVEPRDVELVAS
                     WADALQIGTRNMQNFALLKEVGAASKPVVLKRGLSATVEEWLMAAEYVAQRGNSEIVL
                     CERGIRTFEPSTRFTLDLAGMAVAQQESHLPVIVDPSHATGSKELVAPMARAAVAAGA
                     DGVMIDVHPDPGVALCDGPQALRPEELGELGRQLAELAASLGRQPG"
     gene            complement(990..1784)
                     /locus_tag="VE646_11080"
     CDS             complement(990..1784)
                     /locus_tag="VE646_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80566.1"
                     /translation="MTVGELVFANVVDERVHLSQGDGAPAAIYLQGVPGRALPFVMYR
                     AWKVGTGLVSEEVRFWSPSGRLAYRWGPAVRRMSGSMDLTEERDLVSEARFEESGVHV
                     ASFVLGDEVAAEIEVPVYVQQAPMKLPKQVEDGLRKSDVIFVGDEAKGGHAVPAWFAY
                     KNGRIYVLSRTERGPEEQSVPGIPGATEVLVITRRKANVPETRGRDTALDEFPAVVRT
                     LEGPEWEEAAKVLADRRRSRNGPPEESIQRWRGSCAIAELTPILPG"
     gene            complement(1824..3020)
                     /locus_tag="VE646_11085"
     CDS             complement(1824..3020)
                     /locus_tag="VE646_11085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HZD80567.1"
                     /translation="MNEPQEPREGQPGPLGVPLGDIWNDVPLFREIQRVLLSSSGPVN
                     WELARQIGIASSSWGREDPPPTEEDRRSFEEAVRVAELQVARFTGMEPPSDVAKVDAV
                     RRAQWIQANIEGLKELIEPAAGKLADAVAGAPGSVAPGGVGGFPPQMMQQLSPLLLGA
                     QVGSVLGYLAQHVLGQYDIAVPRSGGGGLLFVVPNIAAFEGDWSLQPTEFRTWVALHE
                     VTHRFEFARPWAAPRFRELLDDFLSTLEVDAGALQARLETLDPSDPEAVQRMFEGEEG
                     LFGTVLDAEQRIKLGRIQAFMSAAEGYGDHVMHAVGSELLSTYGQIEEAMRRYRESEH
                     ADPVFERLLGIEMKREQYRLGKRFCDTVVEQSDEATLARMWDSSEALPSLPELDEPRL
                     WLARTV"
     gene            3071..3493
                     /locus_tag="VE646_11090"
     CDS             3071..3493
                     /locus_tag="VE646_11090"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HZD80568.1"
                     /translation="MQELLVRITGDPLSAQEAVDFVSDPASGATCVFLGTVRDHSGAG
                     SVSGLTYEAWEELAERRLREIGDELFSGWPVRRAALLHRVGDLALEEVSVIVACSCPH
                     RAQAFEACRHGIERLKQDVPIWKKEHLTDGESHWVMGS"
     gene            3493..4539
                     /locus_tag="VE646_11095"
     CDS             3493..4539
                     /locus_tag="VE646_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HZD80569.1"
                     /translation="MGRDIAIDLGTANTLVFRAGEGIVFDEPTVVAMSPSTGRIEAMG
                     EEAWQMIGGRSGSVVAVRPLRHGVVTEFDVTQRMIQVVLRRVGVPRFPKPRAVVCIPS
                     ESSEVERRAVQEAVRFAGGRGVVLVEEALAAAIGAGLPVNEPVGNLIIDIGGGSTEMA
                     VVSMGGVVSGSSVRIGGFDFDAAIQEHVKRTYGVAIGEKAAEELKIAIGSAFPAPHRK
                     SARVTGRALDTGAPVQVRVTDEEVRHAMSEPVSRVVEAARTTLADAPPELTHDVLETG
                     MFLTGGSASLRGLDLLLAQECEVPVHLTDRPLETVVLGAGAMLEHLDDFRASFQLVRR
                     RHPGQERWMRVRRT"
     gene            complement(4545..4931)
                     /locus_tag="VE646_11100"
     CDS             complement(4545..4931)
                     /locus_tag="VE646_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80570.1"
                     /translation="MAILGIALGAGAIVYLLSLRSSDETLALHPVAGDHPEVGDGEPP
                     AEPEPRARRRRGAPRAVGFERSGASASAQAATYVPLAPDETRWQRRVGGLVGLVALVA
                     LAAAVLAGAIYQLGHVINETVARFLQ"
     gene            5018..5995
                     /locus_tag="VE646_11105"
     CDS             5018..5995
                     /locus_tag="VE646_11105"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HZD80571.1"
                     /translation="MRSYRRLALILVPLILVVAATTIWLPYYSLGPGPAREVEPLIHV
                     VGHPTYGSQGRFVMTSVRFSKLTALGALVAWLDPNRSVVGRGTLYAPGETQQQEEVRS
                     RSQMDQSKLAAAYVVLSRLTDYPKGHRPGALVIGTAPGCPADGHLFPGDLITRIDGSR
                     VSGRAQASRIIDRAASGSSLSFDVRAGGQREHVDLVRAPCGGQTDPVVGVVLIPNFPF
                     RVTIESGEIGGPSAGLMWALGLYDLLTPGDLTGGRMIAGTGEIGLDGSVHPISGIGDK
                     IVAAARAGAQVFLLPEGNAKEAGAAGDHGMTLVTVGTFQDALDFLQGSR"
     gene            6114..8978
                     /locus_tag="VE646_11110"
     CDS             6114..8978
                     /locus_tag="VE646_11110"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HZD80572.1"
                     /translation="MRRRWPVVVAAVVVALIIAFTALSGFFVDLLWFREVGFSQVFWT
                     ILWTKVALGVIFGAAFFVLLYANFLIVRAITPRYRALTPEQEVIERYRMAFEPHAWWL
                     LPLIALVIAFFVALGVTAQWQTFLEWRNSSGVSFGTLDPLFHRDAAFYVFTLPWLEFV
                     QGWLFSALVGVTLITAIAHYLWGGIRPQAPGFGEKVTPQVKAHLSVLIGLIILIKAWG
                     YYLGTFDLLSSPRGVVSGASYTDVNAQLPALRILTFIAIACAVLFLVNIRMRGWALPV
                     IAVGLLALVSITAGAVYPAFVQQFRVKPQELQKERPYIEDNISATRQAFGLDAIDTQA
                     RPFTPRVSQQDVKDNDATIQNIRVWRPDVLQENLQSLQRIRQYYEFNDVDVDRYVLDG
                     QRRVVMVSAREVAQGQIPGGGGTWQNQHLFYTHGFGAVATQVNTATTEGAPRFTLQDI
                     PPVGQPQLIGNGQRVYYGELSQVPFVVVNTGTPELDYQGTPQNDAQQVPFRYDGQGGV
                     PMGGIAKRALFAWKYKDVNLLISGLIRGDSRIMIYRDIQTRIPKAAPFLKYDGDPYAA
                     VVDGRLVWIQDAYTTTNRYPYSQPVDLASVTPMSQDRSGLTGSANYIRNSVKVVVDAY
                     NGTMTYYVADPKDPIIQVWHNAFPGLFTKLDDASPDLRDHFRYPENLFQTQMAQYANY
                     HVTNPDVFYGKQDFWQIPTDPTVEFQSGAQVPIRPYYLLMRLPGDTNESFVLIMPFTP
                     QGRQNLVAWTAAKSDPSDYGQIVTFDFPSGQNVFGPTQAFAQINQDPDFSQQRTLLGQ
                     GGSQVIFGDLLVIPINDGLLYVQPVYVRSQQTNSIPELKRALVVNGGNVGIGTNLQGA
                     LAASLGQEAPAGGGGGGGGGGGGGQVSGTVQQQIRSLLQQAADHFARAQAALQAGDLA
                     TYQSEVNAARQAVDRANQLARGRPTSGPTPSGSPSASPTA"
     gene            9034..10683
                     /locus_tag="VE646_11115"
     CDS             9034..10683
                     /locus_tag="VE646_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZD80573.1"
                     /translation="MELEPNMTDYEETRRTFRLAVPARFNYGIDVVDAWAARQPGKLG
                     LLAVGPDGGDPRRYTFEDLCRASNRVANFLAAQGVGRGDRVFVMLHRVPEWHLALLGC
                     IKLGAVPMPGTPLLTERDIAYRIARADAVAAVTDAEGAAKVEAVRDECPSLRTRVCVQ
                     EDRPPGWMSFRDGLERAAETPAAADPTAGDDPMLVYFTSGTTGYPKMVLHTQASYGIG
                     HEVTARFWQDLKATDLHWTFSDTGWAKAAWGKLFGQWRLGAGVFLWDVRGKPDFELAL
                     RLIGRYRVTTFCAPPTVFRAAVLSDLASFDWGSLRHVVSAGEPLNPEVIRQWREATGT
                     TVYDGYGQTETVNVVANYRCLPVREGSMGKPVPGFEVRVVDEDGIEAPPGTAGHIAIR
                     WRPERPVGLFKEYWRDPDATAASFRGDWYYTGDLATADEDGYLWFVGRADDVITSSAY
                     RIGPFEVESAVVEHRAVAEAAVVGKPDPLRGEIVKAYVALRPGYEPSEELARDIQEHV
                     KRVTAPYKYPREIEFVSELPKTVSGKIRRVELRQREIERSK"
     gene            10705..12102
                     /gene="sthA"
                     /locus_tag="VE646_11120"
     CDS             10705..12102
                     /gene="sthA"
                     /locus_tag="VE646_11120"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HZD80574.1"
                     /translation="MTERFDLVVIGAGPAGEKAGAQAAYFGKRVAIVDRRSDPGGSAA
                     SKTGVPTKTLREAALYLTGFRRRDLYGLGMDLTRDTVLERMRTRTAEVVQLAVEAVRR
                     NLERHGIEFVVGEARLGPDRTVLVATEAGERMLRGDVVLVATGSRPYHPPDLFPNDPD
                     VHDSESILELERIPSRLVVIGGGPVGCEYASIFLALGSDVTLVDRGSRLLPFADAELS
                     EALATIFERRGMRLIRQADASGLERVDGALRLSLEDGRTQPTDAVLFAAGRAGSTDGL
                     QLDAAGVKTDERGRILVDDTYRTSAEGIYAAGDVIGPPGLASAAMEQGRVAACHAFAI
                     PFKDTVDPLAPFGVYSVPELGMVGLTEEAAREQGIDVGSGRSPFEANARARIAGAAEG
                     LVKLVFTRQERRLLGVHILGDDASELIHIGQAVLHAGGTIDYFIHSTFNVPTLSEAYK
                     YAAYDGLQRLSGHRL"
     gene            complement(12411..>12974)
                     /locus_tag="VE646_11125"
     CDS             complement(12411..>12974)
                     /locus_tag="VE646_11125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZD80575.1"
                     /translation="DLHDGSQQRLVSLGLELREVTEAIPPHVPEARDRLGRIGEGLDA
                     VLDELRTISRGIHPAILSQGGLVPALKALGRRAAIPVELNVRIDGRLPEPIEVGAYYM
                     ISEALTNVSKHARASVVRIDGEVREGGLHLAIRDDGTGGADPDTGSGLIGLRDRAEAL
                     GGALEIVSPRGGGTTITVELPLSPSLD"
BASE COUNT         1852 a         4504 c         4654 g         1964 t
ORIGIN      
        1 ccagcgcgtg gccgagcgcg tccgggaggt gggtggcgag gccttcgtct cgcggggcgc
       61 cgtgcacacg atcatcggcc tggtggggga cacggcgacc ttccgctccc tcgatctgga
      121 caaccttccc ggggtcgacc acctgatcca gatcggcaag ccctacaaga tggtggcccg
      181 cgcgctgcat ccgcagccga gcaccgtcaa ggtgggaccg gcggcggtgg gtcgggagct
      241 gttcgcactg atcgcgggac cctgcgccgt ggagagcgag gagcaggccc tggcggcggc
      301 ccgagcggcg aaggcagccg gcgccacgat cctgcgtgga ggagcgttca agccccgcac
      361 ctcgccctac tccttccagg gcctgggccg gcagggcctg gagatcctgg cggggtgccg
      421 gcaggagacc gggctgcccg tggtgaccga ggtggtcgag cctcgcgacg tggagctggt
      481 ggcctcctgg gccgacgccc tgcagatcgg gacccgcaac atgcagaact tcgcgctgct
      541 gaaggaggtc ggagccgcca gcaagcccgt ggtcctgaag cggggcctgt cggcgacggt
      601 ggaggagtgg ctcatggccg ccgagtacgt ggctcagcgc gggaactcgg agatcgtcct
      661 gtgcgaacgg gggatccgca ccttcgagcc gtccaccagg ttcacgctgg acctggccgg
      721 tatggccgtg gcccagcagg agagccacct gcccgtgatc gtcgatccct cccacgccac
      781 gggctccaag gagctggtgg cacccatggc ccgcgccgcg gtggccgccg gcgccgacgg
      841 cgtgatgatc gacgtccacc ccgaccccgg ggtcgccctg tgcgacgggc cgcaggcgct
      901 ccgcccggag gagctcggcg agctgggtcg tcagctcgcc gagctggccg cgtccctggg
      961 tcgccagccg ggctgagggt cgacgcgcgc tacccgggaa ggatcggcgt gagctcggcg
     1021 atggcgcagg agccacgcca gcgctggatc gactcctcgg gcgggccgtt gcggctacgg
     1081 cgccggtcgg ccaggacctt cgcggcctcc tcccactcgg gcccctcgag ggtgcggacc
     1141 acggcgggga actcgtccag cgcggtgtcc cggccccggg tctccggcac gttggccttc
     1201 cgccgggtga tcaccaggac ctcggtggct ccggggatcc cggggacgct ctgctcctcg
     1261 ggtccccgct cggtcctgga caggacgtag atccggccgt tcttgtaggc gaaccaggcc
     1321 ggaacggcgt ggccgccctt cgcctcgtcg cccacgaaga tcacgtcgga cttgcgcagc
     1381 ccgtcctcca cctgcttggg cagcttcatc ggcgcctgct gcacgtagac gggcacctcg
     1441 atctcggcgg ccacctcgtc gcccagcacg aacgaggcca cgtggacccc ggattcctcg
     1501 aaccgcgctt cggacaccag gtcgcgctcc tcggtcaggt ccatcgaccc gctcatccgg
     1561 cgcaccgccg gtccccaccg gtaggccaga cgccccgagg ggctccagaa gcggacctcc
     1621 tcgctcacca ggccggttcc caccttccag gctcggtaca tcacgaaggg aagggctcgg
     1681 cccggcacgc cctgcaggta gatggcggcg ggcgcgccgt ccccctgcga caggtggacg
     1741 cgttcgtcca ccacgttcgc gaagacgagc tcaccgacgg tcatcggtcc ggaaggatac
     1801 cggagccggc gccggccgga gcctcagacg gtccgcgcca gccagagtcg cggctcgtcg
     1861 agctcgggta gggaaggcag ggcctcggac gagtcccaca tcctggccag cgtggcctcg
     1921 tcgctctgct cgaccaccgt gtcgcagaag cgcttgccca ggcggtactg ctcgcgcttc
     1981 atctcgatgc ccaggagccg ctcgaagacc gggtcggcgt gctcggactc gcggtaccga
     2041 cgcatggcct cctcgatctg gccgtaggtg gacaagagct cggatcccac cgcgtgcatc
     2101 acatggtcgc cgtagccctc ggcggcggac atgaacgcct ggatccggcc gagcttgatc
     2161 cgctgctccg cgtccaggac cgtcccgaac aggccctcct ccccctcgaa catccgctgc
     2221 acggcctccg ggtccgaggg gtccagcgtt tccagccgcg cttggagcgc gccggcgtca
     2281 acctcgagcg tcgacaggaa gtcgtccagg agctcgcgga accgaggggc cgcccagggc
     2341 cgggcgaact cgaagcggtg ggtgacctca tgcagggcca cccaggtgcg gaactcggtg
     2401 ggctgcaggc tccaatcccc ctcgaacgcg gcgatgttgg ggaccacgaa cagcagcccg
     2461 cccccgcccg accgagggac cgcgatgtcg tactgaccga ggacatgctg ggccagatag
     2521 ccgaggaccg agcccacctg cgcgccgagc agcagcggcg acagctgctg catcatctgg
     2581 ggggggaatc caccgacccc gccgggcgcc accgatcctg gggccccggc caccgcatcg
     2641 gcgagcttgc cggcggccgg ctcgatcagt tccttgagtc cctcgatgtt ggcctggatc
     2701 cactgggcgc gacggacggc gtccaccttg gccacgtcgg agggcggctc catgcccgtg
     2761 aaccgcgcca cctgcagctc ggccacccgg accgcctcct cgaagctccg acggtcctcc
     2821 tcggtgggcg gtggatcctc ccgaccccag gaggaggagg cgatgccgat ctgccgggcc
     2881 agctcccagt tcacggggcc gctggaggac aggagcaccc gctggatctc gcggaacagc
     2941 ggcacgtcgt tccagatgtc gccgagcggc accccgagcg ggccgggctg cccttccctg
     3001 ggctcctggg gctcgttcac atcatccatc ctatccaccc gcctggatca ccctccctag
     3061 aatgggctgc gtgcaggagc tcctggtccg gatcacgggc gatcccctgt cggcgcagga
     3121 ggccgtggac ttcgtctccg atccggcctc cggggccacg tgcgtgttcc tggggacggt
     3181 gcgtgaccac tccggagccg gcagcgtcag cggcctcacc tacgaggcct gggaggagct
     3241 cgctgagcgt cggctccgcg agatcgggga tgagctgttc tcgggctggc cggtccgtcg
     3301 ggcggcgttg ctgcaccggg tgggcgatct ggcgctggag gaggtctcgg tgatcgtggc
     3361 ctgctcgtgc ccccaccggg cccaggcctt cgaggcgtgc cgccatggga tcgagcggct
     3421 gaagcaggac gtcccgatct ggaagaagga gcacctgacc gacggcgagt cccactgggt
     3481 gatgggcagc tagtgggccg ggacatcgcg atcgatctgg ggaccgccaa caccctggtc
     3541 ttccgggccg gggagggcat cgtgttcgac gagcccacgg tcgtggccat gagcccctcc
     3601 accggacgga tcgaggccat gggcgaggag gcctggcaga tgatcggggg ccgctccggc
     3661 agcgtggtgg cggtgcggcc cctccggcac ggcgtggtca ccgagttcga cgtgacgcag
     3721 cggatgatcc aggtggtgtt gcgacgggtc ggcgtccccc ggttccccaa gcccagggct
     3781 gtggtgtgca tcccctcgga gagctcggag gtcgagcgtc gcgccgtgca agaggccgtc
     3841 cgcttcgccg gcggccgggg agtcgtgctg gtcgaggagg cgctggcggc cgcgatcggc
     3901 gcgggcctgc cggtgaacga gccggtcggc aacctgatca tcgacatcgg cggtgggagc
     3961 acggagatgg cggtggtctc gatgggcggc gtggtcagcg gctcgtcggt gcggatcggt
     4021 ggattcgact tcgacgccgc gatccaggag cacgtgaagc ggacctacgg ggtcgccatc
     4081 ggggagaagg cggccgaaga gctgaagatc gcgatcgggt cggccttccc cgctccccat
     4141 cgcaagagcg cgagggtgac cggccgggcc ctcgacaccg gagcgcccgt gcaggtccgg
     4201 gtgaccgacg aggaggtccg ccacgccatg agcgagccgg tgagccgcgt ggttgaggcc
     4261 gcccggacca ccctggcgga cgcgccgccc gagctcacgc acgatgtgct cgagacgggc
     4321 atgttcctca ccgggggaag tgcgtcgttg cgcgggctcg acctgctgtt ggcgcaggag
     4381 tgcgaggtgc cggtccacct caccgatcgt cctctcgaga ccgtggtcct gggggccgga
     4441 gcgatgctcg agcacctgga cgacttccgc gcgtccttcc agctggtgcg gaggcgccat
     4501 cccggccagg agcggtggat gcgcgtgcgc cggacctgac cccgctactg caggaaccgg
     4561 gcgaccgtct cgttgatcac atgcccgagc tggtagatcg cgccggccag cacggcggcc
     4621 gccagcgcta cgagagccac cagcccgacc agcccgccca cccgccgctg ccaacgggtc
     4681 tcgtcggggg ccagcgggac gtaggtggcg gcttgggcgc tggctgaggc cccggagcgc
     4741 tcgaacccta ccgcgcgtgg agcaccacgc cggcgcctgg ccctcggctc gggttcggcc
     4801 gggggctccc catcgcccac ctcgggatgg tcgcccgcca ctggatgaag ggccaaggtc
     4861 tcgtcgctgc tgcgcaggga cagcaggtac acgatcgcgc cggctcccag cgcgatgccg
     4921 aggatggcca tggggtgcat cgtggtctcc gcctctagga cttcgacctt accccggatg
     4981 gttccgttcc gtgcatgggc ccgtagcatg gcccgacgtg cgctcctacc gcaggctcgc
     5041 cctcatcctg gtccccctga tcctggtggt cgcggccacc acgatctggc tgccctacta
     5101 ctcgctcggc cccggcccgg ctcgggaggt cgagccgctg atccacgtcg tgggccaccc
     5161 gacctacgga tcgcaagggc ggttcgtgat gacatcggtc cggttcagca agctgaccgc
     5221 cctgggtgcc ctggtggcct ggctcgaccc gaaccgatcg gtggttggtc gcggcacgct
     5281 ctacgcgccc ggggagaccc agcagcagga ggaggtgcgc agtcgctcgc agatggacca
     5341 gagcaagctc gccgccgcct acgtcgtgct ctctcgactc accgactacc ccaagggtca
     5401 ccggcccggg gcgctggtga tcggtaccgc acccgggtgc ccggccgacg gtcacctgtt
     5461 ccccggcgac ctcatcaccc ggatcgacgg gagccgggtc tcggggcgag ctcaggcctc
     5521 gcggatcatc gaccgggccg cctcgggctc gagcctgtcc ttcgatgtcc gcgccggcgg
     5581 gcagcgagag cacgtcgacc tggtccgggc tccgtgcggc ggtcagacgg acccggtggt
     5641 gggggtggtg ctgatcccca acttcccctt ccgcgtcacg atcgagagcg gggagatcgg
     5701 ggggccgtcg gccgggctga tgtgggctct cgggctgtac gacctgctga cgcccggtga
     5761 tctcaccggc gggcggatga tcgccgggac gggtgagatc gggttggatg gaagcgtgca
     5821 ccccatcagc gggatcgggg acaagatcgt ggccgcggcc cgcgcggggg ctcaggtgtt
     5881 cctcctcccc gagggcaacg cgaaagaggc tggggcggcc ggcgaccacg ggatgaccct
     5941 ggtcacggtg gggacgttcc aggacgccct ggacttcctc cagggctcgc gctgaccggg
     6001 tccctgaggg ggtccccatg cgagccatcg catccgaggg ccggtgatac gctggcgccg
     6061 cttcccgacg accggagaca acgctggcac gcatcagcat cccgcggggc gccgtgcgac
     6121 gccgctggcc cgtggtggtt gcggcggtcg tggtggccct gatcatcgct ttcaccgccc
     6181 tgtccggctt cttcgtggac ctgctgtggt tccgcgaggt cgggttctcg caggtgttct
     6241 ggacgatcct gtggaccaag gtggctctgg gagtgatctt cggagcggcg ttcttcgtgc
     6301 tcctgtacgc gaacttcctg atcgtgcggg ccatcacacc gcggtaccgg gcgctgaccc
     6361 ccgagcagga ggtcatcgag cgctaccgca tggccttcga gccgcatgcc tggtggctcc
     6421 tgcccttgat cgcgttggtg atcgcgttct tcgtggctct cggggtgacc gcccagtggc
     6481 agaccttcct ggagtggcgg aactcctctg gcgtgagctt cgggacgctg gacccgctgt
     6541 tccaccggga cgcggcgttc tacgtcttca cgctaccgtg gctggagttc gtccagggct
     6601 ggctgttctc cgcgctcgta ggcgtgacgc tgatcacggc gatcgcccac tacctgtggg
     6661 gcggcatccg gccccaggcg cccggcttcg gtgagaaggt gactccccag gtcaaggccc
     6721 acctgtcggt gctgatcggc ctgatcatcc tgatcaaggc gtggggttac tacctgggca
     6781 cgttcgacct gctcagctcg ccgcgggggg tcgtctcggg cgcctcgtac accgatgtga
     6841 acgctcagct gccggcgctg cggatcttga ccttcatcgc gatcgcctgc gcggtgctgt
     6901 tcctggtgaa catccggatg cgcggctggg cgctcccggt gatcgcggtg gggctgctgg
     6961 cgctcgtctc catcacggcc ggcgccgtct atccggcgtt cgtccagcag ttccgggtca
     7021 agccccagga gctgcagaag gagcgcccgt acatcgagga caacatcagc gccacgcggc
     7081 aggccttcgg cctagacgcc atcgacaccc aggcccgtcc cttcacgccc cgggtgagcc
     7141 agcaggacgt gaaggacaac gacgccacga tccagaacat ccgggtctgg cggcccgacg
     7201 tgctgcagga gaacctgcag tcgctccagc ggatccgtca gtactacgag ttcaacgacg
     7261 tggacgtgga tcgctacgtc ctggacggac agcggcgagt ggtcatggtc tcggcccggg
     7321 aggtcgcgca gggacagatc cccggcggcg gcggcacctg gcagaaccag cacctgttct
     7381 acacgcacgg gttcggcgcc gtggccacgc aggtgaacac cgctaccacc gagggcgcgc
     7441 cgcggttcac gctccaggac atcccgccgg tgggccagcc tcagctgatc ggcaacgggc
     7501 agcgggtcta ttacggcgag ctgtcccagg tgccgttcgt ggtggtgaac acggggaccc
     7561 ccgagctcga ctaccagggc acgccccaga acgatgccca gcaggtcccc ttccgctacg
     7621 acgggcaggg cggcgtcccc atgggcggga tcgcgaaacg agcgctcttc gcctggaagt
     7681 acaaggacgt caacctgctg atctccggac tgatccgcgg cgacagccgg atcatgatct
     7741 accgggacat ccagacccgg atcccgaagg ccgcgccctt cctgaagtac gacggtgatc
     7801 cgtacgcggc ggtggtggac gggcgcctgg tctggatcca ggacgcgtac accaccacga
     7861 atcggtaccc gtactcccag ccggtggacc tggcgtcggt cacgccgatg tcccaggacc
     7921 gcagcggcct gaccggctcc gccaactaca tccggaactc cgtgaaggtg gtcgtggacg
     7981 cctacaacgg cacgatgacg tactacgttg ccgatcccaa ggaccccatc atccaggtct
     8041 ggcacaacgc cttccccggt ctgttcacga agctcgacga cgcgtccccg gacctgcgtg
     8101 accacttccg gtacccggag aacctattcc agacgcagat ggcacagtac gccaactacc
     8161 acgtgacgaa tcccgacgtc ttctacggga agcaggactt ctggcagatc ccgacggacc
     8221 ccaccgtgga gttccagagc ggggcccagg tgccgatccg cccctactac ctgctgatgc
     8281 ggctgcccgg ggacacgaac gagagcttcg tgctgatcat gccgttcacg ccgcagggcc
     8341 gtcagaacct cgtggcctgg acggcggcga agtccgaccc cagcgactac ggacagatcg
     8401 tgaccttcga cttcccctcc ggtcagaacg tgttcggccc cacccaggcc ttcgcgcaga
     8461 tcaaccagga tcccgacttc tcccagcagc ggaccctgct gggccagggg ggatcccagg
     8521 tgatcttcgg cgacctgctg gtgatcccca tcaacgacgg cctcctgtac gtccagccgg
     8581 tctacgttcg ctcgcagcag accaactcga taccggagct gaagcgggcg ctggtggtga
     8641 acgggggcaa cgtcgggatc gggaccaacc tccagggggc cctggccgcc tccctgggcc
     8701 aggaggcgcc cgccggtggt gggggcggcg gtggtggcgg tggtggtggg ggacaggtct
     8761 cgggcaccgt gcagcagcag atccgctcct tgctgcagca ggccgccgac cacttcgcgc
     8821 gagcccaggc cgccctgcag gcgggcgatc tggccacgta ccagagcgag gtcaacgccg
     8881 cacggcaggc cgtggaccgg gccaaccagc tcgcccgcgg ccggcccacc tcggggccca
     8941 cgccgtcggg ctcgccctcg gcgagtccga cggcctgatc acggcgcccc ggccgcacct
     9001 agcgtgaccg ggtccgcccg ggaaggaggt cacgtggagc tcgagccgaa catgaccgac
     9061 tacgaggaga cccggcggac gttccggctg gccgtccccg cacgtttcaa ctacgggatc
     9121 gatgtggtgg atgcgtgggc ggcccgccag cccggcaagc tcgggttgct ggccgtgggt
     9181 ccagacggtg gcgacccccg acgctacacg ttcgaggacc tgtgccgggc gtcgaaccgg
     9241 gtggcgaact tcctggcggc ccagggcgtg ggccgggggg accgcgtctt cgtgatgctc
     9301 caccgcgtgc ccgagtggca cctcgcgctc ctgggctgca tcaagctggg ggcggtcccc
     9361 atgccgggga cccctctgct caccgagcgg gacatcgcgt atcgcatcgc gcgggccgac
     9421 gccgtggccg cggtgaccga cgccgagggc gcggcgaagg tggaggcggt ccgggacgag
     9481 tgtccctcgc tccgcacccg ggtctgtgtg caggaggatc ggccgccggg ctggatgtcc
     9541 ttccgcgacg gactcgagcg ggcggccgag accccggcgg ctgccgatcc cacggccggc
     9601 gacgatccga tgctcgtgta cttcacctcc ggcaccaccg gctatccgaa gatggtgctc
     9661 cacacccagg cctcgtacgg gatcgggcac gaggtcaccg cccggttctg gcaggacctg
     9721 aaggccacgg acctgcactg gaccttctcg gacaccgggt gggccaaggc ggcctggggc
     9781 aagctgttcg gccagtggcg gctgggggcg ggggtgttcc tgtgggacgt gcggggcaaa
     9841 ccggacttcg agctggccct ccggctgatc ggccggtacc gcgtcaccac cttctgcgcc
     9901 ccgcccaccg tgttccgagc cgcggtgctc tcggacctgg cctcgttcga ctggggctcg
     9961 ctccgccacg tggtctcggc cggggagccc ctgaacccgg aggtgatccg gcagtggcgg
    10021 gaggccaccg ggacgaccgt ctacgacggc tacggccaga ccgagacggt gaacgtggtg
    10081 gcgaactacc ggtgcctgcc cgtccgggag ggctcgatgg gcaagccggt cccggggttc
    10141 gaggtccggg tggtggacga ggatgggatc gaggcgccgc cggggacggc cgggcacatc
    10201 gcgatccgct ggaggcccga gcgcccggtg gggctgttca aggagtactg gcgcgatccc
    10261 gatgcgacgg ccgccagctt ccgcggtgac tggtactaca cgggcgacct ggccacagcg
    10321 gacgaggacg ggtacctctg gttcgtgggc cgggccgacg acgtgatcac ctcctcggcc
    10381 taccggatcg gtcccttcga ggtggagtcg gcggtcgtcg agcatcgggc cgtggccgag
    10441 gcggcggtgg tgggcaagcc cgatccgctg cgaggggaga tcgtgaaggc gtacgtggcc
    10501 ctgcggcctg ggtacgagcc ctccgaggag ctggcgcggg acatccagga gcacgtgaag
    10561 cgggtcacgg ccccgtacaa gtacccccgc gagatcgagt tcgtgagcga gctccccaag
    10621 accgtctcgg ggaagatccg tcgggtggag ctccggcagc gcgagatcga gcggagcaag
    10681 taggcttgcg tccgtgaggc ccacgtgacc gagcgcttcg atctggtcgt gatcggcgcg
    10741 gggccggcgg gggagaaggc cggagcgcag gcggcgtact tcggcaaacg cgtggccatc
    10801 gtggatcgcc gttccgatcc tggcggctcg gcggccagca agaccggtgt gcctaccaag
    10861 accctgcgcg aagccgccct gtacctgacg ggcttccggc ggcgggatct gtacggcctg
    10921 ggcatggatc tcaccaggga caccgtgctc gagcgcatgc gcacgcgaac cgccgaggtg
    10981 gtgcaactgg cggtggaggc cgtgcgccgc aacctggagc gtcatgggat cgagttcgtg
    11041 gtgggcgagg cccggctggg ccccgatcgc accgtcctgg tcgccacgga ggccggtgag
    11101 cggatgctgc ggggtgacgt ggtgctggtg gccaccgggt cgcgccccta ccacccgccg
    11161 gacctgttcc ccaacgaccc cgacgtgcac gactcggaat cgatcctcga gctcgagcgg
    11221 atcccctcgc gcctggtggt gatcggtggg ggaccggttg gctgtgagta cgcctcgatc
    11281 ttcctggcgc tcggctcgga cgtgacgctg gtggaccgcg gctcccgact gctgcctttc
    11341 gccgacgccg agctctccga ggccctcgcc accatcttcg agcggcgggg gatgcgcttg
    11401 atccggcagg ccgacgcgtc ggggctcgag cgggtcgacg gagcgctgag gctctccctg
    11461 gaggacggac ggacgcagcc gaccgatgcg gtcctgttcg cggcaggccg agcgggttcg
    11521 accgacgggc tgcagctcga cgcggccggc gtgaagaccg acgagcgggg gcggatcctc
    11581 gtggatgaca cgtaccggac ctcggccgag ggcatctacg cggccggcga cgtgatcgga
    11641 ccgccggggc tggcctcggc cgccatggag caggggcgcg tggccgcctg tcatgccttc
    11701 gcgatcccgt tcaaggacac cgtcgatccg ctggccccgt tcggggtgta ttcggtgccc
    11761 gagctcggga tggtggggct gaccgaggag gcggcgcggg agcagggcat cgacgtggga
    11821 tcgggccgct caccgttcga ggccaacgcc cgagcccgga ttgccggggc cgcggagggc
    11881 ctcgtcaagc tggtcttcac gcggcaggag cgacgcctgc tcggggtgca catcctggga
    11941 gacgacgcgt ccgagctgat ccacatcggc caggcggtgc tccacgccgg gggcacgatc
    12001 gactacttca tccactccac gttcaacgtg cccacgctgt cggaggccta caagtacgcg
    12061 gcctacgacg gcctgcagcg cctgtccggt caccgcctgt aggggcttcg cggaccgggc
    12121 cgttcggccc tcccgatcca gggcgggcac cccgttggct gtcggggtgt tcttcccttt
    12181 ggacaccctc ccgaaggtgg tggaggcgtc gcgcagacgc tgcccctcac gccgctgctc
    12241 caggcgatgg gcaccgtggt cgtcgacggg cgctcgatca ccgccccggg gccgcaggtc
    12301 ctccacctgg cccggtgggt ggcggcgtcg ttcgcggtag cggcctggag ctttcccctt
    12361 ctccgacggt cggtgaggtt cccggcccgc ctccgccggg ccgcggcggg tcagtccagg
    12421 gacggggaca ggggcagctc gaccgtgatc gtggtgcctc ctccgcgggg gctcacgatc
    12481 tccagcgcgc ctccgagcgc ctccgcccga tcgcggaggc cgatcaaccc cgagccggtg
    12541 tccgggtcgg ctccacccgt cccgtcgtcg cggatggcga ggtggagtcc accctcgcgc
    12601 acctcgccgt cgatccgaac cacggaggcc cgggcatgct tggagacgtt ggtgagcgcc
    12661 tccgagatca tgtagtaggc gcccacctcg atgggctcgg gcaggcgccc gtcgatccgc
    12721 acgttcagct ccaccgggat cgccgcccgc cggcccagtg ccttgagcgc ggggaccagg
    12781 ccaccctgcg agaggatcgc ggggtggatc ccccgcgaga tcgtgcggag ctcgtccagg
    12841 acggcgtcca gaccctcgcc gatccggccc agccgatccc gagcctcggg cacgtgggga
    12901 gggatcgcct cggtgacctc gcgcaactcc aggccgagcg agaccaaccg ctgctggctc
    12961 ccgtcgtgca ggtc
//
LOCUS       DASQQQ010000296         6549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_219776, whole genome shotgun sequence.
ACCESSION   DASQQQ010000296 DASQQQ010000000
VERSION     DASQQQ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219776"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VE646_11130"
     CDS             <1..406
                     /locus_tag="VE646_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZD80576.1"
                     /translation="DLLAALGGLRDDPELSLDFLSDVTATDWPERDPRFWVAYQLYSI
                     RHHHRLRVKVPLGPDEAHVPSVTALFAGADWYEREVYDFFGIVFDGHPDLRRIELPEG
                     WRGHPLRKTEELGGVNTRYHGAFIPPVDRRTT"
     gene            403..1677
                     /locus_tag="VE646_11135"
     CDS             403..1677
                     /locus_tag="VE646_11135"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011597997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HZD80577.1"
                     /translation="MSARARRGLDPEPLGTEGGFRQETMLINMGPQHPSTHGVLRLLL
                     ELDGEEVVSCHPIVGYLHTGIEKNCEYRTWQQGVTYVTRADYLAPFFNELAYCLATER
                     LLGIEAPPRAQVIRVLVCEMNRIASHLVWVATGGMELGAISLMLYGFREREMLLDIFE
                     AITGLRMNHAYIRIGGIVMDLPPGAEGRIRELVPVLRGRIHEYEDILARNPIWVERNR
                     GIGHLGAEQALALGVTGPMLRAAGVPRDVRRDEPYCGYEGYEFDVVTRTEGDCYARFE
                     VRMEEMKQSLRIIEQCLDRLEPGPVMVEDPKVRWPAQLEVGPDGIGNSERYIEHIMEG
                     SMEALIHHFKMVTEGVSVPPGEAYQPVEGPRGELGMYVVSDGGHKPYRVKIRDPSFAN
                     LQSIPTMVQGGLVADVIAVVASVDPVMGGVDR"
     gene            1677..2264
                     /locus_tag="VE646_11140"
     CDS             1677..2264
                     /locus_tag="VE646_11140"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HZD80578.1"
                     /translation="MADRAVLTPELREQAEGIKARYPRGRERSAVMPLLYLMQSVDGR
                     VTREGLQEVADLLGITTAEVEAVATFYTMIRLAPTGRYLLAVCTNLPCALHGGRETLE
                     AARRAAGIPEGEEVSEDGLFTVDEEECLAACDAAPVVSVNFANYDRTSAERVTELIEA
                     LRRGEEPAPSRGPFPGDVRRMSRVLAGLPPEELVP"
     gene            2282..3643
                     /gene="nuoF"
                     /locus_tag="VE646_11145"
     CDS             2282..3643
                     /gene="nuoF"
                     /locus_tag="VE646_11145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HZD80579.1"
                     /translation="MPRVLTGRWDEPDTVWIERYLETGGYQGLREALAHTPAELIDLV
                     KASGLRGRGGAGFPTGVKWSFVPQDTGKPTYLVVNFDESEPGTYNNRELVERDPHQFL
                     EGSIIAAYAVQCHTAFIYCRGEFLWPGMILERVVAQAYDRGFLGKDILGSGYDLDVVI
                     HRGAGAYICGEETALLSSLEGYRGQPRLRPPFPAVEGLYASPTLINNVETLANVPHIL
                     ERGADWFKQWGTEKSPGTKLFSVSGKVERPGNYEAPLGTPARVVIEELAGGVLGGKRL
                     KAWTPGGSSTPFLTEEHLDVGLDFEDIQQAGSLLGTGAIMVCDESDCMVEVTRRLSQF
                     YAHESCGKCTPCREGTWWVIRVLGRIESGYGRPEDIALLEDQCRNMLFRGFCALVDGA
                     VSPIASSLKFFREEYERHIEEHRCPVSGAGPSVASGFGQERDEIPHAAEAPEYMDVAE
                     VLE"
     gene            3640..6045
                     /locus_tag="VE646_11150"
     CDS             3640..6045
                     /locus_tag="VE646_11150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HZD80580.1"
                     /translation="MSPAPDTVTLAIDGKEVTVPKGTLIIRAAEQLGMEIPRFCEHPL
                     LDPIGACRQCYVEIEGQRKLFTSCTTEAADGMAVHTQNTSEEAHEAQIANLEFLLINH
                     PLDCPICDRGGECPLQDQTMAYGPGESRYVEPKRTYEKPVPLSSLVLLDRERCVLCTR
                     CTRFCDQISGDRFIEMFERGAGQQVAIAAGEDLRSPFSGNTIQICPVGALTASTYRFV
                     ARPFDIHSADGVCAHCSAGCNMKVDLRRGQVVRTLARDNLQVNDAWLCDKGRFAFRSA
                     DARDRITTPLIRARGLEPAPFHEVFERIGEWSRGKRVAFLAGGRLTDEDAYALSKLAR
                     TVFGTNDLDHRRHLGGGHAERLTAASPLEVTYRDVERAPVIVVVGLDAEQEVPILHLR
                     VRKAVHTHGARVVVIGPRPTRLRDLAQHVPCLPGEEAHALERLVDADDNHDELGAAAA
                     ALRAAGPNAVVLAGPRVADVPISLDLVMRQAGRVGARACWVSRRAGDRGALRAGVHPE
                     LLPGGRRVTEAEERAEVEAVWGELSNTERGADAHRILEDAAARRIEVLFLIGVDPLHD
                     FPDAALALHALRNVPYKVVQDLGLGQLEPFADAFLPAAAPVEKDGHFSTWEGRPQRTR
                     PVRDPQGLSRPDWEIFAGLAQALGHDLGFGSLDELREELGGLLAPREVRAPDPGYLFL
                     PPVERSEKELILFSYPLLVDEGRLSVDATELKAALEEEAFVEVHPGDAERLGLVEGAR
                     AVVRTEAADVELPVRVDAGLAEGSCFVPFHQPGLAANVLLTGRFSVPATLEPVTAQGE
                     GAA"
     gene            6045..6479
                     /locus_tag="VE646_11155"
     CDS             6045..6479
                     /locus_tag="VE646_11155"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HZD80581.1"
                     /translation="MDWVGWLLLVVRVVIVFFAQLLLVLLLIWMERKVIADMQTRPGP
                     LRAGPRGILITLADGIKLFFKEGIMPSNADRVVYQVAPVAAMLPAFLAFAVVPFGPTI
                     HLFGHRVPFQIADLNIGILWVLAMGSMMVYGIVLAGWSSGSN"
BASE COUNT          924 a         2203 c         2435 g          987 t
ORIGIN      
        1 ggacctgctc gcggccctgg ggggcctccg ggacgatccc gagctgtccc tggacttcct
       61 gtccgacgtc accgccaccg actggcccga gcgggatccg cgcttctggg tggcctacca
      121 gctgtactcg atcaggcacc atcaccggct ccgggtgaag gtgccgctcg gcccggatga
      181 ggcgcacgtg ccctcggtca cggcgctgtt cgcgggcgcc gactggtacg agcgcgaggt
      241 ctacgacttc ttcggcatcg tcttcgacgg ccatccggac ctgcgcagga tcgagctgcc
      301 cgagggctgg cggggtcatc ccctgcgcaa gaccgaggag ctgggcgggg tcaacacccg
      361 gtaccacggg gcgttcatcc cgccggtgga tcggaggacc acgtgagcgc ccgggcccgg
      421 cggggcctcg acccggagcc cctcgggacg gaggggggat tccggcagga gaccatgctc
      481 atcaacatgg ggccgcagca cccctccacg cacggcgtgc tgcgcctgct cctggagctc
      541 gacggtgagg aggtggtctc ctgccacccg atcgtggggt acctgcacac ggggatcgag
      601 aagaactgcg agtaccggac ctggcagcag ggcgtgacct acgtgacccg ggccgattac
      661 ctggccccct tcttcaacga gctggcctac tgcctggcca ccgagcggct gctcggcatc
      721 gaggccccgc cccgggccca ggtgatccgg gtgctggtct gcgagatgaa ccggatcgcc
      781 tcccacctgg tgtgggtggc caccgggggc atggagctgg gggcgatctc gctgatgctc
      841 tacggcttcc gcgagcgcga gatgctcctg gacatcttcg aggccatcac cggcctgcgg
      901 atgaaccacg cctacatccg gatcggcggg atcgtgatgg acctgccacc gggggcggag
      961 ggacggatcc gcgagctcgt cccggtcctg cggggccgga tccacgaata cgaggacatc
     1021 ctggcccgca acccgatctg ggtggagcgc aaccgcggga tcgggcacct cggcgccgag
     1081 caggccctgg cgctcggcgt gaccgggccc atgctgcggg ccgcgggcgt tccccgagac
     1141 gtccgccgcg acgagcctta ctgcggctac gaggggtacg agttcgacgt cgtcaccagg
     1201 accgagggcg actgctacgc gcggttcgag gtgcgcatgg aggagatgaa gcagtcgctc
     1261 cgcatcatcg agcagtgcct ggaccgactg gagcccggac cggtgatggt cgaggacccc
     1321 aaggttcggt ggcccgcgca gctcgaggtg ggaccggacg gcatcggcaa ctccgagcgg
     1381 tacatcgagc acatcatgga gggctcgatg gaggccctga tccaccactt caagatggtg
     1441 accgaggggg tctccgtccc gcccggcgag gcctaccagc cggtggaggg tccccggggc
     1501 gagttgggga tgtacgtggt ctcggacggt ggacacaagc cgtaccgggt gaagatccgc
     1561 gatccctcgt tcgcgaacct ccagtcgatc cccaccatgg tccagggtgg gctggtggcc
     1621 gacgtgatcg ccgtggtggc cagcgtggat ccggtgatgg gcggggtgga ccgctgatgg
     1681 ccgatcgtgc cgtgctgacg ccggagcttc gcgagcaggc cgaggggatc aaggctcggt
     1741 acccacgggg tcgggagcgc tcggcggtga tgccgctgct ctacctgatg cagtcggtgg
     1801 acggccgggt gacgcgtgag ggcctccagg aggtggccga cctgctgggc atcaccaccg
     1861 ccgaggtcga ggcggtggcc accttctaca cgatgatccg gctggccccc accgggcggt
     1921 acctgctggc cgtctgcacg aacctgccgt gcgccctcca cggggggcgg gagacgctcg
     1981 aggcggcccg gcgtgcggcc ggcatcccgg agggcgagga ggtctccgag gacggcctgt
     2041 tcacggtcga cgaggaggaa tgcctggccg cctgcgacgc cgctccggtg gtgagcgtga
     2101 acttcgcgaa ctacgatcgg acgtcggccg agcgggtgac ggagctgatc gaggccctgc
     2161 gccgcggtga ggagccggcg ccctcgcggg gccccttccc cggtgacgtc cgccggatgt
     2221 cgcgggtcct ggccgggctg ccccccgagg agctggtgcc gtgagtgccg cgagccccga
     2281 gatgccgcgg gtgctgaccg gccggtggga cgagcccgac acggtctgga tcgagcggta
     2341 cctggagacc ggggggtacc aggggctgcg cgaggcgctc gcgcatacgc cggccgagct
     2401 gatcgatctg gtcaaggcgt cgggcctgcg gggccggggc ggtgcggggt tccccaccgg
     2461 cgtgaagtgg tccttcgtgc cacaggacac gggcaagccc acctacctcg tggtgaattt
     2521 cgacgagtcg gagccgggca cctacaacaa ccgcgagctc gtggagcgcg acccccacca
     2581 gttcctggag ggctcgatca tcgcggcgta cgcggtgcag tgccacaccg ccttcatcta
     2641 ctgtcggggc gagttcctgt ggccggggat gatcctggag cgcgtcgtgg cccaggccta
     2701 cgacaggggc ttcctgggca aggacatcct cggcagcggt tacgacctgg acgtggtgat
     2761 ccatcgggga gccggggcgt acatctgcgg cgaggagacg gcgctcctgt cctccctgga
     2821 gggataccgg gggcagcccc gcctgcgtcc gccgttcccg gccgtggagg ggctgtacgc
     2881 gagccccacc ctgatcaaca acgtcgagac cctggccaac gtgccgcaca tcctggagag
     2941 gggcgcggac tggttcaagc agtggggcac cgagaagtcc cccgggacca agctgttctc
     3001 ggtctcgggc aaggtggagc gtcccggcaa ctacgaggca ccgttgggca ccccggcccg
     3061 cgtggtgatc gaggagctgg ccgggggggt cctgggaggc aagcggctca aggcctggac
     3121 gccgggcggc tcgtccaccc cgttcctgac cgaggagcac ctcgacgtgg gtctggattt
     3181 cgaagacatc cagcaggccg gctcgctcct ggggacgggc gcgatcatgg tctgcgacga
     3241 gagcgactgc atggtggagg tcacgcgccg cctctcccag ttctacgccc acgagtcatg
     3301 cgggaagtgc acgccgtgcc gtgagggcac gtggtgggtg atccgggtcc tggggcggat
     3361 cgagtccggc tacggacgcc ccgaggacat cgcgctgctc gaggaccagt gccggaacat
     3421 gctgttccgg ggcttctgcg ccctggtgga cggggccgtc tcgcccatcg cctcctccct
     3481 gaagttcttc cgggaggagt acgagcggca catcgaggag catcggtgcc cggtgagcgg
     3541 cgcgggcccc agcgtggcct ccgggttcgg gcaggagcgc gacgagatcc cgcatgcggc
     3601 cgaggccccc gagtacatgg acgtggccga ggtgctcgag tgagcccggc accggacacg
     3661 gtgacgctcg cgatcgacgg caaggaggtg accgtgccca agggcacact catcatccgc
     3721 gccgccgagc agctggggat ggagatcccc cggttctgcg agcatccgct gctggacccc
     3781 atcggggcct gccggcagtg ctacgtggag atcgagggtc agcgcaagct gttcacctcg
     3841 tgcaccacgg aggcggcgga cggcatggcc gtccacaccc agaacacctc ggaggaggcc
     3901 cacgaggcgc agatcgccaa cctcgagttc ctgctgatca accatcccct ggactgcccg
     3961 atctgcgatc ggggcggcga gtgcccgctg caggaccaga ccatggccta cggtccgggg
     4021 gagagccgct acgtcgagcc caagcggacc tacgagaagc cggtgcccct ctcctcgctc
     4081 gtgctgctgg accgggaacg atgcgtgctg tgtacccgct gcacgcggtt ctgcgaccag
     4141 atctcgggcg accgtttcat cgagatgttc gagcgggggg ccggccagca ggtggccatc
     4201 gccgccggcg aggacctgcg ctcacccttc tccgggaaca cgatccagat ctgcccggtc
     4261 ggtgcgctca cggcgtcgac gtaccgcttc gtggcgcgtc ccttcgacat ccacagcgcg
     4321 gacggggtgt gcgcgcactg ctccgcgggc tgcaacatga aggtggacct gcgccggggg
     4381 caggtggtcc gcaccctggc tcgggacaac ctgcaggtga acgacgcctg gctctgcgac
     4441 aagggccggt tcgccttccg ctccgccgat gcccgggacc gcatcaccac gccgctgatc
     4501 cgggcccgag ggctcgagcc ggcccccttc cacgaggtgt tcgagcggat cggcgagtgg
     4561 tcccggggca agcgggtcgc cttcctcgcc ggcggtcggc tcaccgacga ggacgcctat
     4621 gccctgtcca agctggcccg gaccgtcttc ggtacgaacg acctggacca ccggcggcac
     4681 ctgggcggcg gccacgccga gcgcctgacc gccgcctcgc ctctggaggt cacctaccgc
     4741 gacgtcgagc gggccccggt gatcgtggtg gtgggcctcg acgccgagca ggaggttccc
     4801 atcctccacc tgcgggtccg caaggccgtt cacacccacg gcgctcgggt cgtggtgatc
     4861 gggccccgtc ccacccggct gagggacctg gcgcagcacg tgccctgcct gcccggcgag
     4921 gaggcccacg ccctggagcg cttggtggac gcggacgata accacgacga gctgggcgcc
     4981 gccgccgcgg ccctgcgggc tgcgggcccg aacgccgtgg tcctggccgg cccccgggtg
     5041 gccgacgtgc cgatctcgct ggatctggtg atgcgtcagg caggccgggt gggcgcccgc
     5101 gcctgctggg tgagccgccg agccggtgac cggggagcgc tgcgggcggg cgtgcatccc
     5161 gagcttctgc ccggcgggcg ccgggtgacc gaggccgagg agcgggccga ggtggaggcg
     5221 gtgtggggag agctctcgaa caccgagcgg ggagccgatg cccaccggat cctggaggac
     5281 gccgccgccc ggcggatcga ggtcctgttc ctgatcggcg tggatccgct ccacgacttc
     5341 cccgacgccg cgctcgccct ccacgcgctg cggaacgtgc cctacaaggt ggtgcaggac
     5401 ctggggctgg gacagctgga gcccttcgcc gatgccttcc tgccggcggc ggcccccgtg
     5461 gagaaggacg gtcacttcag cacgtgggag ggccggcccc agcggacccg accggtgcgg
     5521 gacccccaag ggctgtcccg gccggactgg gagatcttcg ccggtctggc ccaggcgctg
     5581 ggacacgacc tggggttcgg ctcgctggac gagctgcggg aggagctggg cgggctgctc
     5641 gcgccgcgcg aggtccgtgc ccccgacccc gggtacctgt tcctgccccc ggtggagcgg
     5701 agcgagaagg agctgatcct gttcagctac ccgctgctgg tggacgaggg ccgtctatcg
     5761 gtggacgcca ccgagctcaa ggccgccctg gaggaggagg cgttcgtcga ggtgcacccc
     5821 ggggacgccg agcggctggg gctggtcgag ggggcgcggg cggtggtccg cacggaggcg
     5881 gccgacgtgg agctcccggt ccgggtggat gcgggcctgg ccgagggctc gtgcttcgtg
     5941 cccttccacc agccggggct cgccgccaac gtgttgctga ccgggcggtt cagcgtgccg
     6001 gccacgctgg agccggtgac cgcccagggc gagggagcgg cctgatggac tgggtgggct
     6061 ggctgctgct ggtggtgcgc gtggtgatcg tcttcttcgc ccagctgctg ctggtgctcc
     6121 tcctgatctg gatggagcgc aaggtcatcg ccgacatgca gacccggccc ggaccgcttc
     6181 gggccggacc ccgtgggatc ctcatcaccc tggcggacgg gatcaagctg ttcttcaagg
     6241 agggcatcat gccctcgaac gccgaccgcg tcgtgtacca ggtcgcgccc gtcgcggcga
     6301 tgctgccggc cttcctggcc ttcgccgtgg tgccgttcgg gcccacgatc cacctgttcg
     6361 gtcatcgggt gcccttccag atcgccgacc tcaacatcgg gatcctgtgg gtcctggcca
     6421 tgggctcgat gatggtctat gggatcgtgc tggccggctg gtccagcggg tcgaactaac
     6481 cgctcctcgg ctcgatccgg tcttcggccc agatgatcag ctacgaggtg gggatgggcc
     6541 tggcgctgg
//
LOCUS       DASQQQ010000297        18569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_162955, whole genome shotgun sequence.
ACCESSION   DASQQQ010000297 DASQQQ010000000
VERSION     DASQQQ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 18569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18569
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162955"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VE646_11160"
     CDS             <1..441
                     /locus_tag="VE646_11160"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HZD80582.1"
                     /translation="ADQHIDHRSIQDHVGSRTSSDLLYKGALKGRSNAINVGTVIIRE
                     GAHLCDAYQTNRNILLSETAKAHSVPNLEILTNDPTRCGHAASVGPVSEDEIFYLMSR
                     GIPAQEAERLIVFGFFQEVLDRIQLEEIRAGLERTIEAELTREG"
     gene            443..769
                     /locus_tag="VE646_11165"
     CDS             443..769
                     /locus_tag="VE646_11165"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HZD80583.1"
                     /translation="MALVRICGVNDVPVGEAGRFELDHRPLAVVNLGEEGFRVVDAIC
                     SHAHYFLDEGEVDPDFETIECPKHGSTFDLNTGRARTLPATAPVQVFNVKVDDEDVLI
                     EIEESA"
     gene            781..1554
                     /gene="sufC"
                     /locus_tag="VE646_11170"
     CDS             781..1554
                     /gene="sufC"
                     /locus_tag="VE646_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HZD80584.1"
                     /translation="MATATLEIRGLRASVEGKEILKGIDLVVKQGETHALMGPNGSGK
                     STLANVLMGRPGYQILDGHVLFKGEDVATLRPDQRAQRGLFLAMQYPVEIPGVSVVNF
                     LRTAYKSVKGEEISALAFRKHMKEKMNLLGVEDAMVQRYVNQGFSGGEKKRNEILQLA
                     VLEPQIAVLDETDSGLDIDSLKQVATGVAQLIGPSLGVLLITHYQRILNYITPDFVHV
                     MMGGRIVKSGGKELAHELEDKGYEGIRAELGVEKGADEE"
     gene            1576..2829
                     /locus_tag="VE646_11175"
     CDS             1576..2829
                     /locus_tag="VE646_11175"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HZD80585.1"
                     /translation="MSVTQERAPGALDVARIRADFPILSRTVAGRPLVYLDSAATSQK
                     PRQVIEAERSYYEQHNANAHRGIYLLAEESTELYEGARAKLAAFLGAPGPSSIVFNRG
                     TTESINLVAHGWGRRVLQPGDEILLTEMEHHSNLVPWQLCAKDTGAVLRYIPLTDDGL
                     LDLSNLEELLSERTKILCVTGMSNALGTVTPLPTLIDAAHAVGAVVLVDGAQFVPHTK
                     VDVTALGCDFLAFSGHKMLGPTASGGLYGKPELLERMEPMFGGGEMIREVYHDHATWN
                     EVPFKFEAGTMNIAQEVALGAAVDYLEGLGMENVRAHEEQISAYAIGRLTQAGARIFG
                     PTDAAIRGGAVSFWYKDVHPHDLATILDEEGVAIRAGHHCAQLVMRRYGVPATARASF
                     YLYNTKDEVDALVAALERAEAVFGS"
     gene            2882..3331
                     /locus_tag="VE646_11180"
     CDS             2882..3331
                     /locus_tag="VE646_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006447379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HZD80586.1"
                     /translation="MALDEIYKEVILDHYKNPRNKRELPGAERSCSRNNPLCGDEITV
                     FVHETEGRIADVTFQGQGCSISQSSASMMTQAVSGKTLDEVQTMIRDFRGMMSGDVEP
                     SEDEFGDLVSLKGVVKYPIRIKCAVLAWDVLQEALGSDGEAAGAAAH"
     gene            complement(3328..4074)
                     /locus_tag="VE646_11185"
     CDS             complement(3328..4074)
                     /locus_tag="VE646_11185"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZD80587.1"
                     /translation="MGETPALYDELAEWWPLLSAPQDCADEADEYRRLLEDTVDGPVH
                     EVLELGSGGGNNASHLKRHFRMSLVDRSPGMLAVSRSLNPECEHLEGDMRTIRLSRVF
                     DGVFLHDAVSYLTTREDLGAAMETAAAHCRAGGAALFVPDFVRETFFEGTGHGGHDAP
                     DHGMRYLEWRTDPVPADTTYVVDFAYLLRLPGGAMDVRRDHHVCGLFPRATWLALLEG
                     AGFDARVVPLGHGDLVGSEAFIARRPRPKR"
     gene            4137..5939
                     /locus_tag="VE646_11190"
     CDS             4137..5939
                     /locus_tag="VE646_11190"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HZD80588.1"
                     /translation="MTHGGRSTWLAAAGIMLILGALATPAAAQGERIPRYDVEIRIQR
                     DDSLLVRETIVYDFGSERRHGIFRDVPTTLRFDDSYDRRYPITIVSVEGSAGTPDRFS
                     TESIQGGETRIRIGDPDREIIGRHTYRITYRIAGALNGFQDHDELYWNAIGDEWPVPV
                     EGASVRVIAPGRIQRVACFAGPTGSNLPCASATLHGMTASFDQRDLPAYEALTVVVGL
                     PKGVVDPPSPILVERWSLDRAFTLTPAIAGGSFGLLLVLLAGVGTLVWLTGRDRRYVG
                     SEVDVLMGAPAGTPDQAVPLFEGGTGPVEFAPPEGLRAGQMGTLLDEVANPLDVTATI
                     VDLAVRGYLVIEEIPKEHFWSRADWSLGKRKEPDQDLMEYERTLLTGLFRGGDRVQLS
                     GLKATFVERLRKVQDALYRNASAQGWFVGRPDKVRERWRLVGFGVVAAGAALEYAAVR
                     WTRLGLLPVPVILAGLLMVVGARWMPRRTPKGTGLARRVKGFRTVIATAERYTARWAE
                     QENLFTRFLPYAIVFDLTDKWAKAFEGLAREPDTSWYVSGRPFSAHAFSESIDGFTVS
                     TAGTIASTPSGSGSSGFGGGSAGGGGGGGGGGSW"
     gene            complement(5895..6239)
                     /locus_tag="VE646_11195"
     CDS             complement(5895..6239)
                     /locus_tag="VE646_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80589.1"
                     /translation="MGAVTLGAGSGSTAGSMGGVPPVCLQAIRAARAELGLRSELPQA
                     YVQLFQRAVDAVRRGDTAALEEIAGELQDLNRRAQQGAQATLGDAFSRPARACESLSP
                     GPPTPSAATASG"
     gene            complement(6413..8542)
                     /locus_tag="VE646_11200"
     CDS             complement(6413..8542)
                     /locus_tag="VE646_11200"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZD80590.1"
                     /translation="MSGLVPPRSRALGAEIASIRIFGFPLLSRTMVVQRDRSGLDPLA
                     QARVLLRVVNLDQHRYRGLRRIAGGLPVIDAPQIFPGNRGRPTTAITYLFFRPDVGLS
                     LRDLLAHKFARLRVDQPGDALVGVTGAVPGRLAQGREIRNTLPLVELITVLVVVTIVA
                     WHYRSAVAPLITLLAAAVSFAITERAVPWALGRLGVATPTELEPILVALLLGIVTDYS
                     IFFLSGLRNRLAAGDEPLPAAQTSVADVAPIVLTAGLTVAAGAGSLLLSRLGAFRGLG
                     PGLALTVLMGMLVSVTLVPATMALLGRLAFWPSVPVEGAGEPTRTRHAPTRWVARRNS
                     AAVVAVLCLASLGILGAEIRHLRPSFSVITDLPAGTEVRRAATAAGRGFAPGVTSPTM
                     LVLRGPRARNVARLIRLQDLLQIQPGVAVVLGPREVSAIAGIVDRLPEGRLRNRLEGE
                     ADRVLRAVVSERHRAARLMMILDHPALSADAVETIRRLVATTPSLLDRADLPGVRFAY
                     AGDTALASETIRRTVSDLIRIALVVVVIDLMILAVFLRSLVAPLYLLVTSLLGFLAGL
                     GLTDLAFRTFGGTTGMSIFVPFASAVLLVSLGSDYNIFLVGQVWEEARRSSLHQGLVV
                     AAPRASRAIAAAGITLTFSFAVLALVPLTSFREFAVVMATGIVLDTFVVRTFLVPAMI
                     TLVGRASAWPARFHAPGATVSGPTPPG"
     gene            complement(8722..9333)
                     /locus_tag="VE646_11205"
     CDS             complement(8722..9333)
                     /locus_tag="VE646_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80591.1"
                     /translation="MDAGAFPLLHGRRRVRRMDAIIIAWVVLWFGLSGWIGYEIWTLQ
                     RLSDTLVSSSRALDDTSSLLGRFADLPLVGGSVTQVQRRIEETAGSARLSAASTRNNF
                     ERLSLLLAVAVFILTVIPIVVAYLPHRLSWGRDRRTVRRLLASSTDEGWVQEQLARRA
                     IQKLPYHVLSGITEDPWGDVRARRYEDLADAELNRLGLGSSPD"
     gene            9418..9981
                     /locus_tag="VE646_11210"
     CDS             9418..9981
                     /locus_tag="VE646_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZD80592.1"
                     /translation="MFLRENEEGRRIAPHLSRGQCLLDLGAGTGFMARWLRQAAGVEP
                     TLCDLVDYHNRDRSLPFILQTDPARVPVPDGSFDVVLLMFVFHHIERWADQEALLDEA
                     VRIARHRIVIAEDTPGSRVDRAFNVAWDWLLNWRHGVPKPFTFRAVDGWMELFTTRDL
                     SVAHAETYRPVWPTLATYPHTLFVLDR"
     gene            9986..10978
                     /locus_tag="VE646_11215"
     CDS             9986..10978
                     /locus_tag="VE646_11215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZD80593.1"
                     /translation="MRIVFSDDGRDFVGRDWSPLVEADPAGTFFHTPRFLKLYWEEFG
                     REPAHLLLAFAEDGRGTQVGAVAFERDGRTLRFLGGTEVTDYQGPVAVPEVADAFAKE
                     LLAALLRLDDWSEADLRGLPEDRAWLQRLAGAAEAQGLGVAISDEQNGVAPFLPLPGS
                     YQEYLEVLPPKLRHEIRRKARKLESEAGPYRIHVAHRETLGALLDRFVELHRTSEGPK
                     GVFMQPGMEIFFRRLGQEFLPLGIFNLAFIEVQGEQVAGTIAFRFGGTYFLYNSAFDH
                     RWRQLAPGMVLVAEDIRIAIEGGCTAFDLLKGDYGYKYRFGAYPRSIKRLMVRR"
     gene            complement(10988..11698)
                     /locus_tag="VE646_11220"
     CDS             complement(10988..11698)
                     /locus_tag="VE646_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZD80594.1"
                     /translation="MTCDEVREQLAEHLLGTLEASRDEQVQGHLRGCGSCRRELRALE
                     EGIGTFARAAHQVEPPPEVRDRVLSVLEEEWAASTPEPAHHRHPPWLAWAAAAVVLAG
                     SLAWGGMATVRAGRFQATADKYERFLGVLGGENVRVGTLRPSGGQAVEGSAVLYDSKV
                     EQSWVLILARAPGVQGQASVTLSTADGRTIDMHPLEFGSGGEASSWLVSSSDLRPFDR
                     VTIRDASGRVIASGTVRQ"
     gene            complement(11688..12218)
                     /locus_tag="VE646_11225"
     CDS             complement(11688..12218)
                     /locus_tag="VE646_11225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZD80595.1"
                     /translation="MRAFGERDPAAAEALYSRYASRVYGLGIVMLGNDQAAQDLVQDT
                     FVKLWRSADRFDERKGRLDTWVLLVARSLAIDGLRRRVLQARTLEALGPAPEASPEPG
                     PDDLAATEDMAARARRAMAGLSDGQRAALELAYFGGKTSAEVAELEGIPVGTAKTRIR
                     SALLKLREALEVERDV"
     gene            complement(12329..13159)
                     /locus_tag="VE646_11230"
     CDS             complement(12329..13159)
                     /locus_tag="VE646_11230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HZD80596.1"
                     /translation="MTPDRTQLLGIASAERARLGRTIQYASPDSWEAPSACPGWRNRD
                     VMAHLVAQEYAAAQVVAGEEAQEFAGFRRANDGDFWVEGFNEWAVGVRSEQSSRQIIT
                     DWGRAADLFLSRLADIPPTEWDQRRVPWVAGDIGVRYLVQSRIVEWWLHGEDIREGAG
                     LQENLQHWPLFLIIDMGIRMLPYALGLSGLSFPGRSLQVDLEGVGGGNWHWGLAPREN
                     PSEEKKPDAFIEGRASAFALVAGRRVPADHVLDDGNLVIGGDEDIAQAVLRNIRAFVE
                     "
     gene            13242..14366
                     /locus_tag="VE646_11235"
     CDS             13242..14366
                     /locus_tag="VE646_11235"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HZD80597.1"
                     /translation="MRVLVAPDKFRGTLTAIQAARAIATGWRRARPGDEVVELPMGDG
                     GEGTLDALVEALGGDRRRVPVRGPLGDPTTAELGLVPGASGLTGVVEMARASGLQLLA
                     ESRRDPLRTTTLGTGDLIRAACAEHPAEVLVCIGGSATNDGGAGMAQALGIRLLDAEG
                     RDITTGGAALLDLARIDAGGLDRSCRSVRFVVASDVDNPLTGPEGASAVYGPQKGAGP
                     EDVVLLDRALGHLAAVVHRDLGIDVRALPGAGAAGGLGAGLVAFLGAHIRPGVDLVME
                     AVGFAERLQVADVVVTGEGSLDEQSLRGKVPAGVLRVCREARVRALILSGRAGVHPEG
                     VPVASLVERFGTRRAMEEARPALEDLAAEIAAGWSGLSFG"
     gene            14366..14866
                     /locus_tag="VE646_11240"
     CDS             14366..14866
                     /locus_tag="VE646_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015609100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HZD80598.1"
                     /translation="MSRAVDRFVDAAGIMGHRVEVRRFPQGTRTASEAAAAIGCRVEQ
                     IVKSLVFMADGRPVLALTSGANRVDTARLATLAGAAEVRRASPDEARAATGYAIGGTP
                     PLGCEPRSGERLRVFIDRDLMSFDVVWAAAGAPDAVFPLSPDELVRLSGGAVIGFAQM
                     SSPEEP"
     gene            15128..15445
                     /locus_tag="VE646_11245"
     CDS             15128..15445
                     /locus_tag="VE646_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HZD80599.1"
                     /translation="MQLTEMAAGKVKELLEEEGRSDIALRVAVQPGGCSGLRYAMYLD
                     DQLSDSDLAEDQFGVRVVVDKMSAPYLSQATIDFVDSLEATGFTIDNPAAQGSCACGH
                     SFH"
     gene            15614..15766
                     /locus_tag="VE646_11250"
     CDS             15614..15766
                     /locus_tag="VE646_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80600.1"
                     /translation="MDAIPFTPHLYHQLHDWIFMGAIVVAFAIPQIVAMIRHRSWRHQ
                     VMSAFR"
     gene            complement(15779..17029)
                     /locus_tag="VE646_11255"
     CDS             complement(15779..17029)
                     /locus_tag="VE646_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZD80601.1"
                     /translation="MGRFWHPFADMAATETEGELVIERGEGVHVWDEGGRRYLDATAA
                     LWYCNVGYGREEIAAAAGAQLRKLPAYSAFGDLATRPAIELAERVAAIAPVADSRVFL
                     TSGGSDSIDTAAKIARRYWQLEGQTDRTLFVTRERAYHGMHIAGTSLAGIDANASGYG
                     ALVGDVVRVAWDSPQELRDAIAFAGPERVAAFFCEPIIGAGGVFAPPQGYLDEVRQIC
                     RETGVLFVADEVITGFGRVGEWFASTRWDLQPDLMTCAKGITSGYLPMGAVVASPRVA
                     DAFWRPGAGMFRHGYTYSGHAAVAAAALANLQILEREGLPKRALQLEGDLAGALAPLL
                     EHPLVEEIRSGTGVVAAVQLSTGAIRDDPALPAKAVAACRDAGIMTRALGTGAMQVSP
                     ALVIDGAELVELQEGLSAALDEVA"
     gene            complement(17121..>18569)
                     /locus_tag="VE646_11260"
     CDS             complement(17121..>18569)
                     /locus_tag="VE646_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015622448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HZD80602.1"
                     /translation="EELGLAQGEPAGRILLATVKGDVHDIGKNIVGVVLGCNGYEVID
                     LGVMVPADRLLDTARDEGCDIVGVSGLITPSLDEMVGVAKEMERRGLDLPLLIGGATT
                     SRQHTAVKIAPEYGNPTVHVIDASRVVGVVSNLLDPGRRETFDRENREEQDRLRRQHA
                     ERDLRPLLRYEQATANRTPIVWHQEDLSAPGFIGPRVVDASIQALRSYIDWTFFFTAW
                     ELKGRYPAILQHPRYGAAATELFEHANELLDRIVSDGSIRARGVYGFWPAWAEGDDIV
                     LGSGVRFPMLRQQVDHGDGRPNRSLADYVAPVEMGLRDHVGAFAVTAGLGVDELVARF
                     EADHDDYRAIMVKALADRLAEAFAEQLHERARRDWYETGPKMPAEDLVAERYRGIRPA
                     FGYPACPDHSEKRTLFELLEAGDLGMELTETCAVTPAASVSGLYLGHPAARYFNVGRI
                     GADQVQEYARRRGFGRAEAERWLRPNLAYDPD"
BASE COUNT         2815 a         6421 c         6680 g         2653 t
ORIGIN      
        1 gccgaccagc acatcgacca ccggtcgatc caggaccacg tgggctcgcg gacctccagc
       61 gacctgctgt acaagggcgc gctcaagggc cgctcgaacg ccatcaacgt gggcacggtg
      121 atcatccgcg agggggcgca cctctgcgac gcctaccaga ccaatcggaa catcctgctg
      181 tccgagacgg ccaaggccca ctcggtcccc aacctggaga tcctcacaaa cgaccccacc
      241 cgctgcggtc acgcggccag cgtgggtccg gtgagcgagg acgagatctt ctacctcatg
      301 agccgcggga tcccggcgca ggaggccgag cggctcatcg tgttcggctt cttccaggag
      361 gtgctggacc gcatccagct ggaagagatc cgcgccggcc tggagcggac catcgaggcc
      421 gagctcacga gggaggggtg acgtggcgct ggtccgcatc tgcggggtga acgacgtccc
      481 ggtcggcgag gccgggcgtt tcgagctcga ccatcggccg ctggccgtgg tgaacctggg
      541 cgaggagggc ttccgtgtcg tggacgcgat ctgctcgcac gcccattact tcctggacga
      601 gggcgaggtc gacccggact tcgagaccat cgagtgcccc aagcacgggt ccacgttcga
      661 cctcaacacc ggccgggccc ggaccctgcc ggccaccgcg ccggtccagg tgttcaacgt
      721 gaaggtggac gacgaggacg tgctgatcga gatcgaggag agcgcatgag cgacacggcg
      781 gtggccacgg ccacgctcga gatccgaggc ctgcgggcct cggtggaggg caaggagatc
      841 ctgaagggga tcgacctggt ggtcaagcag ggagagaccc acgccctgat gggcccgaac
      901 ggctcgggga agtccaccct ggcgaacgtg ctgatgggcc gccccggcta ccagatcctg
      961 gacgggcatg tcctgttcaa gggggaggac gtcgccacgc tcaggccgga ccagcgggcc
     1021 cagcgcgggc ttttcctggc catgcagtac ccggtggaga tccccggggt ctcggtcgtg
     1081 aacttcctgc gcaccgctta caagtcggtg aagggagagg agatctcggc gctggcgttc
     1141 cgcaagcaca tgaaggagaa gatgaacctc ctcggcgtcg aggacgccat ggtgcagcgc
     1201 tacgtgaacc agggcttctc cgggggggag aagaagcgca acgagatcct ccagctggcc
     1261 gtgctggagc cccagatcgc ggtgctggac gagaccgatt cgggcctgga catcgactcg
     1321 ctgaagcagg tcgccaccgg ggtggcgcag ctgatcgggc cgagcctggg ggtcctgctc
     1381 atcacgcact accagcggat cctcaactac atcaccccag acttcgtcca cgtgatgatg
     1441 ggtggtcgta tcgtgaagtc gggaggcaag gagctggcgc acgagctcga ggacaagggc
     1501 tacgagggca tcagggccga gctcggagtg gagaagggcg cggacgagga gtgagatgag
     1561 gagggaggcg gcgcgatgtc ggtgacccag gagcgcgccc cgggcgcgct ggacgtggct
     1621 cgcatccggg ccgacttccc gatcctgtcc cggacggtgg ccggtcggcc cctggtgtac
     1681 ttggacagcg cggcgacctc gcagaagccg cggcaggtca tcgaggccga gcggagctac
     1741 tacgagcaac acaacgcgaa cgcgcaccgc ggcatctacc tgctcgcaga ggagtccacc
     1801 gagctgtacg aaggggcccg agccaagctg gcggcgttcc tcggcgcgcc cggcccgtcc
     1861 tcgatcgtct tcaaccgggg cacaaccgaa tcgatcaacc tcgtggcgca cgggtgggga
     1921 cgccgggtcc tgcaacccgg cgacgagatc ctgctcaccg agatggagca ccactcgaac
     1981 ctcgtcccgt ggcagctctg cgcgaaggac accggggcgg tcctgcgcta catcccgctc
     2041 accgacgatg gcctcctgga cctatccaac ctggaggagc tgctcagcga gcgaaccaag
     2101 atcctatgcg tcaccggcat gtcgaacgcg ctgggcacgg tcacgccgct gcccaccctg
     2161 atcgacgcgg cgcacgccgt gggggcggtg gtcctggtcg acggtgccca gttcgtcccg
     2221 cacacgaagg tggacgtcac cgccctgggc tgcgacttcc tggccttctc gggacacaag
     2281 atgctggggc caacggcctc ggggggtctg tacggcaagc ccgagctgct ggagcggatg
     2341 gagcccatgt tcggtggggg cgagatgatc cgcgaggtct accacgacca cgccacctgg
     2401 aacgaggtgc cgttcaagtt cgaggccggg acgatgaaca tcgcgcagga ggtcgccctg
     2461 ggggcggcgg tcgactacct ggaggggctc gggatggaga acgtccgggc ccacgaggag
     2521 cagatcagcg cgtacgcgat cgggcggttg acccaggccg gggcccggat cttcggtccg
     2581 accgatgcgg ccatccgggg tggagcggtt tccttctggt acaaggacgt ccacccgcac
     2641 gatctggcca ccatcctgga cgaggagggc gtggcgatcc gggccggtca tcactgcgcg
     2701 cagctggtga tgcgccggta cggggtcccg gcgacggcgc gagcgagctt ctacctgtac
     2761 aacaccaagg acgaggtgga cgcgctcgtt gccgcgctgg agcgcgcgga agcggtgttc
     2821 ggctcctagc gttggtaccg taggcgcggt ccgccgctcg ggccgcccgg agggatggaa
     2881 cgtggcgctc gacgagatct acaaggaagt catcctcgac cactacaaga acccgcggaa
     2941 caagcgcgaa ctccccgggg ccgagcgctc gtgctcccgc aacaacccgc tgtgcggcga
     3001 cgagatcacg gtcttcgtgc acgaaaccga gggcaggatc gccgacgtga cgttccaggg
     3061 gcagggctgc tcgatctccc aaagctcggc gtcgatgatg acccaggcgg tctcggggaa
     3121 gaccctggac gaggtgcaga ccatgatccg cgacttccgg ggcatgatgt cgggcgacgt
     3181 ggagccctcc gaggacgagt tcggtgatct cgtgtccctg aagggcgtgg tgaagtaccc
     3241 gatccggatc aagtgcgccg tgctcgcctg ggacgtcttg caggaggcgc tgggctccga
     3301 cggcgaggcc gccggcgccg ccgcacacta gcgcttcggt cggggcctgc gggcgatgaa
     3361 ggcctcgctg cctaccagat ctccgtgtcc gagggggacc acgcgcgcat cgaagccggc
     3421 tccctccagg agtgccagcc aggtcgcccg ggggaagagc ccgcacacgt ggtggtccct
     3481 ccgcacatcc atggccccgc caggcaggcg gagcaggtac gcgaaatcca cgacgtacgt
     3541 ggtgtcggcg gggaccgggt cggtccgcca ctccaggtac cgcatgccgt gatccggcgc
     3601 gtcgtggccg ccgtggccgg taccttcgaa gaaggtctcg cgcacgaagt cggggacgaa
     3661 gagggcggcc cccccggccc ggcagtgggc ggcggccgtc tccatcgcgg cccccaggtc
     3721 ctcccgagtg gtgaggtacg agacggcgtc gtggaggaag accccgtcga agacccggct
     3781 cagcctgatc gtgcgcatgt cgccctcgag gtgctcgcac tcggggttca gggagcggct
     3841 gacggcgagc atgcccggcg agcgatccac caggctcatg cggaagtggc gcttcaggtg
     3901 tgacgcgttg ttgccgccgc cggagccgag ctcgaggacc tcgtgcaccg ggccgtcgac
     3961 cgtatcctcg agcagccgac ggtattcgtc ggcctcgtcc gcgcagtcct ggggggcgga
     4021 caacaggggc caccactcgg ccagctcgtc gtacagggcc ggcgtctcac ccatgcaggg
     4081 aggctacctc cggccccggc gcggtgcgag gcccggcggc gtataggctt cgaccgatga
     4141 cccacggggg gaggtcgacc tggctcgccg ccgcggggat catgctcatc ctgggggccc
     4201 tcgccacacc ggccgcggcg cagggcgagc ggatcccgcg ctacgacgtg gagatcagga
     4261 tccagcgcga cgactcactc ctggtccggg aaaccatcgt ctacgacttc ggctcggaac
     4321 gccggcacgg gatcttcagg gacgtcccca cgacgcttcg cttcgacgac tcgtacgacc
     4381 ggcgctatcc gatcaccatc gtgtcggtcg aaggctcggc cggaacgccc gaccggttca
     4441 gcaccgagtc gatccagggc ggcgagacca ggatcaggat cggggatccg gaccgtgaga
     4501 tcatcgggcg gcacacgtat cgcatcacct accgcatcgc cggggcgctg aacgggttcc
     4561 aggaccacga tgagctgtac tggaacgcga tcggcgacga gtggccggtg ccggtggaag
     4621 gtgcctccgt ccgggtgatc gcccccggac gcatccagcg ggtcgcgtgc ttcgcgggac
     4681 cgaccggctc caacctgccg tgcgcctcgg ccacgctgca cgggatgacg gcctcgttcg
     4741 accagcgcga cctgcccgcg tacgaggccc tgacggtggt ggtgggtctg ccgaagggtg
     4801 tggtggatcc accatccccg atcctggtgg agcgatggtc gttggaccga gcgttcaccc
     4861 tcactccggc tatcgccggt gggtccttcg gcctgctgct ggtgctgctc gccggtgtag
     4921 ggacgctggt ctggctgacg gggcgggacc gccggtacgt aggctcagag gtcgacgtgc
     4981 tgatgggggc gcccgccggg acccccgacc aggccgttcc gctgttcgaa ggcgggacgg
     5041 gacccgtgga gttcgccccg ccggagggtc tccgggccgg gcagatgggc accctcctgg
     5101 atgaggtggc gaaccccctg gacgtcacgg ccaccatcgt ggacctggcc gtgcgcgggt
     5161 acctggtgat cgaggagatc cccaaggaac acttctggag cagggcggat tggtccctgg
     5221 gcaagcgcaa ggagcccgac caggacctga tggagtacga gcggacactg ctcacgggcc
     5281 tgttccgggg tggagaccgg gtccagctct cggggctcaa ggcgacgttc gtggagcggc
     5341 tgcgaaaggt ccaggacgcg ctgtaccgca acgcatccgc tcagggctgg ttcgtgggcc
     5401 ggccagacaa ggtccgcgag cgctggcggc tcgtcggttt cggcgtggtg gcggcgggcg
     5461 cggcgttgga gtacgcggcc gttcgctgga cccggctcgg cctgctcccg gtcccggtga
     5521 tcctcgcggg cctgctgatg gtcgtcgggg cccgatggat gcctcggcgc accccgaagg
     5581 ggaccggcct ggcccgacgc gtgaaggggt tccgtacggt gatcgcgacc gccgagcggt
     5641 acacggcgcg atgggccgag caggagaacc tcttcacccg gttcctgccc tacgcgatcg
     5701 tcttcgacct taccgacaag tgggccaagg cgttcgaggg gctggcgcga gagcccgaca
     5761 cctcctggta cgtgagcggc cggccgttct cggcacacgc gttctcggag tcgatcgacg
     5821 ggttcacggt gtccacggcg ggcaccatcg cgtcgacccc gtccggctcc ggctccagcg
     5881 ggttcggggg cggctcagcc ggaggcggtg gcggcggagg gggtgggggg tcctggtgac
     5941 agggactcgc aggccctcgc cggccggctg aaggcgtcgc ccagcgtggc ctgcgccccc
     6001 tgctgggcac gtcggttcag gtcctgcagc tctcccgcga tctcctcgag ggcggccgtg
     6061 tcgcctcgcc ggacggcgtc gaccgcccgc tggaacagct ggacgtaggc ctggggaagc
     6121 tcggatcgga gcccgagctc ggcccgagcc gcccggatgg cctgcaggca gaccggggga
     6181 acgccgccca tcgacccggc ggtcgagccg gaacctgcgc ccagggtcac agcccccacg
     6241 ccgaacccga tgacgaacga gaccagtgcg ggaccgacga tccaggccca ccacgctcgt
     6301 cgcgagggat cggtgcgcga ggtccgtggc tctggttcga ctgggtgggg ttccatggcg
     6361 cgtgctccgg ctccgcgatg gacctcccgt acctacccga agctccctcc gcctacccgg
     6421 ggggcgtcgg tccggacacc gtggctcccg gcgcgtggaa ccgtgccggc caggcgctcg
     6481 cccttcccac gagcgtgatc atcgccggca ccaggaaggt acggaccacg aaggtgtcga
     6541 gcacgatgcc cgtcgccatg accaccgcga actcgcggaa ggaagtgagg ggcaccagag
     6601 ccaagacggc gaagctgaag gtgagcgtga tccccgccgc ggcgatcgcc cggctggccc
     6661 tcggcgctgc caccaccagc ccctggtgga gggacgatcg gcgggcctcc tcccacacct
     6721 gtcccaccag gaagatgttg tagtccgagc cgagggagac gagcaggacc gccgaggcga
     6781 acggtacgaa gatgctcatg cccgtggtgc cgccgaacgt ccggaaggcc aggtcggtca
     6841 ggccgagccc ggccaggaag ccgagcaggc tggtaaccag gaggtagagg ggcgccacca
     6901 gcgagcggag gaacaccgcc aggatcatca ggtcgatcac caccacgacc agggcgatcc
     6961 tgataaggtc gctcacggtc cgccggatcg tctccgaggc gagcgccgtg tcacccgcgt
     7021 aggcgaaccg cactccgggc agatcggccc gatcgagcag cgaaggggtg gtcgcgacga
     7081 ggcggcggat cgtctccacc gcgtcggcgc tcagcgcggg gtgatcgagg atcatcatca
     7141 ggcgggccgc gcggtgccgc tcggacacga cggcccgcag gacccggtcg gcttcgccct
     7201 ccagtcggtt gcgcaggcgt ccctccggca ggcggtccac gatacctgcg atcgccgaga
     7261 cctcccgggg cccgaggacc acggcaacgc ctggttggat ctgcaggagg tcctggagcc
     7321 ggatcaggcg agcgacgttc cgggcccggg gtccgcggag gacgagcatc gtgggagagg
     7381 tgacacccgg ggcgaacccc cgtccggcgg cggtcgcggc acggcggacc tcggtcccgg
     7441 ccgggaggtc ggtgatcacg ctgaagctcg gccgcaggtg ccggatctcg gcacccagga
     7501 tccccaggct ggccaggcag agcaccgcca cgaccgcggc gctgttgcga cgcgccaccc
     7561 accgggtcgg cgcgtgacgc gtccgggtcg gctcgcccgc gccctcgacc ggcaccgagg
     7621 gccagaaggc caggcgtccc aggagcgcca tcgtggcggg gacgagggtg acggacacga
     7681 gcatccccat gagcacggtc agggccaagc ccggccccaa ccctcggaag gccccgagcc
     7741 gggacaggag gagcgaaccg gcgccggcgg ccacggtgag cccggccgtg aggacgatgg
     7801 gcgccacgtc cgccaccgac gtctgcgcgg ccgggagcgg ttcgtcgccc gcggccaagc
     7861 ggttccggag gcccgatagg aagaagatcg agtagtcggt cacgatcccc agtaggagcg
     7921 ccacgaggat cggctccagc tcggtgggcg tcgccacccc cagccgtccc agggcccaag
     7981 ggaccgcgcg ctccgtgatc gcgaacgaga ctgcggcggc gagcagcgtg atgagcggag
     8041 ccacagcgga gcggtagtgc caggcgacga tcgtcacgac caccagcacg gtgatcagct
     8101 caacgagggg caacgtgttc cggatctcac gcccctgagc cagccgcccg ggcaccgcac
     8161 cggtcacccc gaccagggcg tcaccgggct gatcgacccg gaggcgagcg aacttgtggg
     8221 ccagcaggtc gcggagcgac aggccgacgt cgggccggaa gaacaggtag gtgatcgcgg
     8281 tggtgggtcg gccacggttc cccgggaaga tctgcggggc gtcgatcacc ggaaggccgc
     8341 cggcgatccg ccgcaggccg cggtagcgat gctggtcgag gttgaccacc cgcaggagga
     8401 cccgtgcctg ggccaggggg tcaagcccgg accgatcgcg ttgaaccacc atggtgcggg
     8461 acagcagggg gaacccgaag atccttatgg aggcgatctc cgccccgagg gcgcgtgatc
     8521 gcggtggcac caggccgctc aacccacccg acctcgagcc cccgatccct gggagcatga
     8581 gcaccgccac caccgatgcc ccgacccatc ccagcgagac ggggacccgc agggcgacga
     8641 tccaacgagc gtacgctcgg gaggcccgtc gcgtcggacg ctcggtgggg cgggcgctcc
     8701 caggctcgcc gccggccacg atcagtcggg agaggagccg agcccgagtc ggttcagctc
     8761 agcatccgcc aggtcctcgt accgacgcgc gcggacgtcg ccccaagggt cctccgtgat
     8821 gccgctgagc acgtggtagg gcagcttctg gatcgcccgg cgggccagct gttcctgaac
     8881 ccacccctca tcggtggagg aggcgaggag acgccgcacc gtccgccgat cgcgtcccca
     8941 tgagaggcgg tgcggcaggt acgcgaccac gatcgggatc acggtgagga tgaagaccgc
     9001 gacggcgagg agcaggctca agcgttcgaa gttgttgcgg gtggacgcgg cgctcaggcg
     9061 cgcgctcccg gcggtctcct cgatccttcg ctgcacctgg gtcacgctcc cgcccaccaa
     9121 gggcagatcc gcgaacctcc ccaggaggct cgaggtgtcg tccaacgctc ggctcgagga
     9181 cacgagggtg tcgctcaggc gctgcagggt ccagatctcg tacccgatcc aaccggagag
     9241 cccgaaccag agcaccaccc aggcgatgat gatggcgtcc atccggcgca cgcgtcgtcg
     9301 gccgtgcagg agcgggaacg ccccagcgtc catgtgcgct ctgtacccac gtggtcggat
     9361 gcctaccctg gcggcggcgt ctccatggca acgagccggc tcttgtacgc tcatcgcatg
     9421 ttcctgcggg agaacgagga agggcgtcgg atcgccccac acctctcgcg cggacagtgc
     9481 ctgctggacc tgggagcggg cacggggttc atggccaggt ggctcaggca agccgccggc
     9541 gtggagccca cgctgtgcga cctggtcgac taccacaacc gcgaccggtc gctcccgttc
     9601 atcctccaga ccgatccggc ccgcgtgccg gtgccggacg gatccttcga cgtggtgctg
     9661 ctgatgttcg tcttccacca catcgagcgg tgggccgatc aggaggcgct gctcgacgag
     9721 gccgtccgga tcgcgcggca ccgcatcgtg atcgcggagg acacccccgg gtcgagggtg
     9781 gatcgggcct tcaacgtggc ttgggactgg ctgctgaact ggaggcacgg cgtccccaag
     9841 cccttcacct tccgcgccgt ggacgggtgg atggagctgt tcaccacacg ggacctgtcg
     9901 gtcgcacacg ccgagacgta tcgaccggtc tggcccacgc tggccaccta cccgcacacg
     9961 ttgttcgtgc tggaccgata ggacgatgcg gatcgtcttc tcggacgacg ggcgcgactt
    10021 cgtggggcgg gattggtctc cgctcgtgga ggccgatccg gcggggacct tcttccatac
    10081 cccgcggttc ctgaagctgt actgggagga gttcgggcgg gagccggcgc atctgctgct
    10141 cgctttcgcc gaggacgggc gcgggacgca ggtgggggcg gtagccttcg agcgggacgg
    10201 gcggacgctg cggttcctgg gcgggaccga ggtgacggac taccagggcc cggtcgcggt
    10261 gcccgaggtg gccgacgcgt tcgccaagga gctgctcgcg gccctgctcc gcctggacga
    10321 ttggtcggag gcggatctcc gggggctccc ggaggatcgg gcctggctcc aacgactggc
    10381 cggggcggcc gaggcccagg gcctgggtgt cgcgatcagc gacgagcaga acggtgtggc
    10441 ccccttcctc ccactgcccg gctcgtacca ggagtacctc gaggtcctgc cccccaagct
    10501 gcggcacgag atccggcgca aggcccgcaa gctcgagtcc gaggccggtc cctaccggat
    10561 ccacgtggcg catcgggaga ccctgggcgc gctgctagac cggttcgtgg agctccaccg
    10621 caccagcgag ggcccgaagg gcgtgttcat gcaaccgggg atggagatct tcttccggag
    10681 gctcgggcag gagttcctcc cgttggggat cttcaacctg gccttcatcg aggtgcaggg
    10741 ggagcaggtg gccgggacca tcgccttccg cttcgggggc acgtacttcc tgtacaactc
    10801 ggccttcgac catcggtggc gacagctggc gcccgggatg gtcctggtgg ccgaggacat
    10861 ccggatcgcc atcgagggcg ggtgcaccgc cttcgacctg ctgaagggcg actatgggta
    10921 caagtaccgg ttcggggcgt acccgcggtc catcaagcgt ttgatggtcc gccgatgagg
    10981 cgcctagcta ctgccggacg gtgccgctcg cgatgacacg gccgctcgcg tcccggatcg
    11041 tcacccgatc gaagggccgc aggtccgaag agctcacgag ccagctcgat gcctccccgc
    11101 cactaccgaa ctccagcggg tgcatgtcga tcgtccgccc gtcggccgtc gacagggtga
    11161 ccgaggcctg gccctgcacc ccgggagccc gggccaggat cagcacccag ctctgctcca
    11221 ccttcgagtc gtagagcacg gcgctgccct ccacggcttg gccaccgctg ggtcgcagcg
    11281 tgcccacccg tacgttctcg ccgcccagca caccgaggaa ccgctcgtac ttgtcggccg
    11341 tggcctggaa ccggccggcg cgcacggtgg ccatgccccc ccaggccagg gatcccgcca
    11401 gcacgacggc tgcggcggcc caggccagcc acggcgggtg ccggtggtgg gccggttccg
    11461 gcgtggaggc cgcccactcc tcctccagga cgctcaggac ccggtcccgg acctcgggcg
    11521 ggggctcgac ctggtgcgcg gctcgggcga aggtgccgat accttcctcc agggctcgca
    11581 gctcccgccg gcacgatccg cagccccgca ggtggccctg cacctgctcg tctcgcgagg
    11641 cctccagggt tcccaggagg tgctcggcca gctgctcacg gacctcgtca cacgtcacgt
    11701 tcgacctcca gcgcttcccg aagcttcagc agcgcggatc ggatccgggt cttggcggtt
    11761 cccacgggga tcccttccag ctcggccacc tcggcgctcg tcttgccgcc gaagtacgcg
    11821 agctccaggg ccgcccgctg gccgtccgag aggccggcca tggcccgccg tgcccgggcg
    11881 gccatgtcct cggtcgccgc gaggtcgtcg gggccgggct cgggcgaggc ctcgggcgcc
    11941 ggcccgagcg cctccagcgt ccgagcctgg aggacccggc gacgcagacc gtcgatcgcg
    12001 aggctccggg ccaccaggag gacccaggtg tccagtcgcc ccttccgctc gtcgaagcga
    12061 tcggcgctcc tccacagctt cacgaaggtg tcctgcacca gatcctgcgc cgcctgatcg
    12121 ttgccgagca tcacgattcc caacccgtag accctggagg cgtagcggga gtacagggcc
    12181 tcggcggcgg ccgggtcccg ctctccgaac gcccgcatca gctgggcgtc cgaaccgggg
    12241 tcggaaggac gcactctcag cacgttagtt gttgggagta cgaacctcgg cggggccggg
    12301 ttcactcccg gcggcgggat ccacgcggct actcgacgaa tgctcggatg ttccgcagga
    12361 cggcctgggc gatgtcctcg tccccaccga tcaccaggtt cccatcgtcc aggacgtgat
    12421 cggcgggcac gcgccgcccc gccaccaggg cgaaggcgga ggcccgaccc tcgatgaagg
    12481 cgtccggctt cttctcctcc gatgggttct ctcggggagc caggccccag tgccagttcc
    12541 ctccgcccac gccctccagg tccacctgca ggctccgacc ggggaacgac aggccggaga
    12601 gcccgagagc gtagggcagc atcctgatcc ccatgtcgat gatcaggaac agcggccagt
    12661 gctggaggtt ctcctgcagc ccggccccct cccggatgtc ctcgccgtgc agccaccact
    12721 cgacgatccg cgactgcacc aggtagcgga ccccgatatc cccggccacc cacggcaccc
    12781 ggcgctggtc ccactcggtc ggcgggatgt cggccagacg ggacaggaac aggtccgcgg
    12841 cccgccccca gtcggtgatg atctgccggg aagactgctc ggagcgaacc cccaccgccc
    12901 actcgttgaa gccttcgacc cagaagtcgc cgtcgttggc tcgccggaac ccggcgaact
    12961 cctgcgcctc ctcgccggcc acgacctggg cggccgcgta ctcctgggcg acgaggtggg
    13021 ccatcacgtc gcggttgcgc caaccggggc aggcgctcgg tgcctcccac gagtcggggg
    13081 aagcgtactg gatggtgcgt cccagccgcg ctcgctcggc gctggcgatc cccagcagct
    13141 gcgtgcgatc cggggtcacg tctagcctgc tccttcctcg gccgttcggg gtccgcgtcc
    13201 ggtcctcggc accgggcgcg gcggaatcgt accgtacggc catgcgggtc ttggtggctc
    13261 ccgacaagtt ccgcggcacg ctcacggcga tccaggcggc ccgggccatc gcgaccgggt
    13321 ggcgacgcgc caggcctgga gacgaggtgg tcgagctgcc gatgggggac ggcggggagg
    13381 gcacgctcga cgcgctggtc gaggcgctcg gcggcgaccg gcggcgggtc ccggtgcggg
    13441 gaccgctggg ggaccccacg accgcggagc tcgggctggt gcccggtgcc tcagggctca
    13501 cgggggtcgt cgagatggcc cgggcctcgg gcctgcagct gctcgccgag tcccgccggg
    13561 acccgctacg gaccaccacg ctcggcaccg gcgacctgat ccgcgcagcc tgcgcggagc
    13621 acccggccga agtgctggtc tgcatcgggg gcagcgccac gaacgatgga ggcgcgggga
    13681 tggcccaggc cctggggatc cggctcctgg atgcggaggg gcgcgacatc acgaccgggg
    13741 gtgcggcgct cctggatctg gcccggatcg atgcgggagg gctggaccgc tcctgccggt
    13801 cggtgcgttt cgtggtggcg agcgatgtgg acaacccgct caccggtccc gagggcgcct
    13861 cggccgtcta tggaccgcag aagggcgcgg ggcccgagga cgtggtgctc ctggatcgag
    13921 ccctggggca cctggccgca gtggtgcatc gcgatctggg gatcgacgtg cgggccctcc
    13981 ctggagccgg ggccgcgggg gggctcggcg ccggcctggt cgccttcctg ggggcccata
    14041 tccgccccgg ggtcgacctg gtgatggagg ccgtggggtt cgccgagcgc ctgcaggtcg
    14101 ccgatgtggt ggtgacggga gagggatccc tggacgagca gtcgctccgg ggcaaggtcc
    14161 cggccggtgt cctgcgggtc tgccgggagg cgcgggtcag ggccctgatc ctctccggcc
    14221 gcgcgggggt gcatccggag ggcgtgccgg tggcgtccct ggtcgaacgg ttcgggacgc
    14281 ggcgtgcgat ggaggaggcc cgtcccgcgc tcgaggatct ggccgccgag atcgcggcag
    14341 gctggtccgg gctatcgttc ggctgatgag ccgagccgtg gaccgtttcg tcgatgccgc
    14401 cgggatcatg ggccaccggg tcgaggtgcg ccgcttcccg caggggacca ggaccgcgtc
    14461 ggaggcggcc gcggcgatcg gttgccgggt ggagcagatc gtcaagtcgc tggtgttcat
    14521 ggccgacggt cggcccgtcc tcgccctcac ctcgggggcg aaccgcgtgg acaccgctcg
    14581 gctggccacg ctcgccggcg ccgccgaggt gcgccgtgcc tcccccgacg aagcccgtgc
    14641 cgctaccggc tatgcgatcg gcgggacgcc gcctctgggt tgtgaacctc gttccggaga
    14701 gcggctccgc gtgttcatcg accgggacct catgtccttc gacgtggtgt gggcggcggc
    14761 cggtgcgccc gacgcggtgt tcccgctgag tcccgacgag ctggtgcggc tgagcggagg
    14821 agcggtcatc gggttcgccc agatgagctc gccggaggag ccctaggctt cggcggtccg
    14881 tggaacggga ccgcaggcgc tgggtccgtt cgggagggtt ccgccgatcc cactacctgg
    14941 ccggcgacct ggcgctcctg agcgagatct gggccgcggc cgggaccccc cgaggccgtc
    15001 tttccgcacc acgccgcagg agcaggtggc cgcgacgggg ctcgggcggt cgatctgcgg
    15061 gaaacctgac tggttcggtc gtatttgcgg gtagaatcgc tgaagaccca gctacggagg
    15121 aacgaccatg cagctcaccg agatggccgc cggcaaggtg aaggagctcc tcgaggagga
    15181 gggtcgctcg gacatcgccc tgcgggtggc cgtgcagccc gggggatgct cgggcctccg
    15241 ctacgcgatg tacctggacg accagctctc ggactcggat ctggcggagg accagttcgg
    15301 cgtccgggtg gtggtggaca agatgagcgc tccctacctg agccaggcca ccatcgactt
    15361 cgtcgacagc ctcgaggcta ccggcttcac catcgacaac cccgccgcgc agggctcctg
    15421 cgcctgcggt cactccttcc actgagcgac caccgctgag cggaccaccg cccggtgcag
    15481 cgccgcagac ccaggtcccg gttctagggt cgtcgtccta gggcatcggc gcgccggtcc
    15541 cggtggaagg acggcttggg gccccaggcg ccctcatcat gcggtggcac ggtggacgtg
    15601 gaggttgatg agcatggacg cgatcccctt cacgccgcac ctgtatcacc agctgcacga
    15661 ctggatcttc atgggggcga tcgtcgtggc cttcgcgatc ccgcagatcg tcgcgatgat
    15721 ccgccaccgc agctggcggc accaggtcat gtcggcgttc cgctaacgcg gcaccgggtc
    15781 aggccacctc gtcgagggcc gcggaaaggc cctcttggag ctcgaccagc tcggcaccgt
    15841 cgatcaccag agccggcgaa acctgcatcg ctccggtccc gagcgcccgg gtcatgatgc
    15901 cggcgtctcg gcaggccgcg acggccttgg cgggcagcgc cgggtcgtcg cggatggccc
    15961 cggtggacag ctgcacggcc gccaccaccc ccgtccccga gcggatctcc tcgaccagag
    16021 ggtgctcgag caagggagca agcgctccag ccagatcacc ttccagctgg agggcgcgct
    16081 tgggcagtcc ctcgcgctcc aggatctgaa ggttcgccag ggcggccgcc gccaccgccg
    16141 cgtgccccga gtacgtgtag ccgtggcgga acatccccgc accgggacgc cagaacgcat
    16201 ccgccacgcg cggtgacgcc accaccgcgc ccatcgggag gtagccgctg gtgatgcctt
    16261 tggcgcaggt catgaggtcg ggttgaaggt cccaccgggt gctggcgaac cactcgccga
    16321 cccgtccgaa cccggtgatg acctcatcgg ccacgaacag cacgccggtc tcacggcaga
    16381 tctggcggac ctcgtccagg tagccctggg gcggtgcgaa cacgccgccg gcgccgatga
    16441 tgggctcgca gaagaaggcc gccacgcgct cgggtcccgc gaaggcgatg gcgtcgcgaa
    16501 gctcctgcgg cgagtcccag gccacccgca ccacgtcgcc caccagcgcg ccgtagccgg
    16561 acgcgttggc gtcgatcccg gccaggctgg tgccggcgat gtgcataccg tggtaggccc
    16621 gttctcgggt cacgaacagg gtgcggtcgg tctgcccctc caactgccag tagcgccggg
    16681 cgatcttcgc cgccgtgtcg atcgagtccg atccaccgct ggtgaggaac acgcggctgt
    16741 ccgcaaccgg cgcgatggcc gctactcgct cggccagctc gatggccggc cgcgtggcca
    16801 ggtcgccgaa ggccgaatag gcgggcagct tccggagctg cgcaccggcc gccgccgcga
    16861 tctcctcgcg cccgtagccc acgttgcagt accagagggc cgcggtggcg tcgaggtagc
    16921 gacgtccgcc ctcgtcccac acgtggacgc cctcaccccg ctcgatcacc agctcgccct
    16981 cggtctccgt ggcggccatg tcggcgaagg gatgccagaa ccggcccatg gagggcccac
    17041 cctaccagtg cggacgggcc agagcgcacc tcccggcgga gcagcgcgta tagctgggtt
    17101 ccggcccgga cccgaggagc tcagtccggg tcgtaggcca ggttcgggcg gagccagcgc
    17161 tccgcctcgg cccgaccgaa gcccctgcgc cgggcgtact cctggacctg atcggccccg
    17221 atccgcccta cgttgaagta ccgcgcggcc gggtgtccca gatacagacc cgacacgctg
    17281 gcggccggcg tcaccgcgca ggtctccgtg agctccatgc cgaggtcgcc cgcttcgagc
    17341 agctcgaaga gcgtgcgctt ctcgctgtga tcgggacacg ccgggtagcc gaacgcaggg
    17401 cggatgcccc ggtatcgctc ggccaccagg tcctccgcgg gcatcttggg accggtctcg
    17461 taccagtccc ggcgggcccg ctcgtgcagc tgctcggcga acgcctccgc cagccggtcg
    17521 gcaagcgcct tgaccatgat cgcccggtag tcgtcgtggt cggcctcgaa ccgcgccacc
    17581 agctcatcca cgcccaggcc ggccgtcacc gcgaacgcgc ccacgtggtc gcgcaatccc
    17641 atctcgacgg gagccacgta gtcggccagt gagcggttcg gtcgaccgtc cccgtggtcc
    17701 acctgctggc ggagcatcgg gaaccggacg ccggagccca ggacgatgtc gtccccctcg
    17761 gcccaggccg gccagaaccc gtagacgcca cgggcccgga tggacccgtc ggagacgatc
    17821 cggtcgagca gctcgttcgc gtgctcgaag agctcggtcg cggcggcccc gtaccggggg
    17881 tgctgcagga tggccgggta ccgtcccttc agctcccagg cggtgaagaa gaacgtccag
    17941 tcgatgtagg accgcagggc ctggatcgac gcgtccacca ctcgcgggcc gatgaacccc
    18001 ggggcggaca ggtcctcctg gtgccacacg atcggcgtgc ggttggcggt ggcctgctcg
    18061 tagcggagga gcggtcggag gtcgcgctcg gcgtgttgcc gacggagtcg gtcctgctcc
    18121 tcccggttct cgcggtcgaa cgtctcccgc cggccggggt cgaggaggtt cgagaccacg
    18181 cccaccaccc gggacgcgtc gatcacgtgg accgtcgggt tgccgtactc gggggcgatc
    18241 ttcaccgccg tgtgctgcct cgaggtggtg gcgccgccga tcagcagggg caggtccagg
    18301 ccccgtcgct ccatctcctt ggccacaccg accatctcgt ccagcgaggg cgtgatcagc
    18361 ccggagacgc cgacgatgtc gcagccctcg tcccgcgccg tgtccaggag ccggtcggcg
    18421 ggcaccatga cgccgaggtc gatcacctcg tagccgttgc agccgaggac gacgccgacg
    18481 atgttcttgc cgatgtcgtg aacgtcgccc ttaaccgtgg cgagcaggat ccggcccgcg
    18541 ggctcgccct gcgccagccc cagctcctc
//
LOCUS       DASQQQ010000298         5633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_191288, whole genome shotgun sequence.
ACCESSION   DASQQQ010000298 DASQQQ010000000
VERSION     DASQQQ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191288"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VE646_11265"
     CDS             complement(<1..835)
                     /locus_tag="VE646_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80603.1"
                     /translation="MPRRVLIMGAAGRDFHNFNAVYRDDEGSEVVAFTATQIPFISDR
                     RYPSELAGPRYPEGIPIRDESELSSLIRDLGVQDVVFAYSDVSHEYVMHKGSEALAAG
                     ANFLLLGPRQTMLTAQVPVVAVCAVRTGSGKSQTTRAVAQVLRDAGKRVVAVRHPMPY
                     GDLNAQRVQRFGTLEDGEQVRGKRVLVVEDGPTLTHGEMKFGAGVVAARRNGAAEIVD
                     PRPYAVGTIEETYRKYDVGPVLPAMGYSDQQLREMEKMIDAAPVDLVLIASPIDLRHV
                     IN"
     gene            complement(947..1525)
                     /locus_tag="VE646_11270"
     CDS             complement(947..1525)
                     /locus_tag="VE646_11270"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HZD80604.1"
                     /translation="MTGVVLAGGRSRRFGRDKLAEPYRGTSILQWTVSRLAEVCDEVL
                     VVVGPGQRPPLPSDLPIRLARDDVADQGPLAGVAAGLPACRTTLALVAGGDMPELSAV
                     VLGAMLEEMADPGVPAVVLEDGDRFRPLPCALRVVPAVEAAAALLADGERRLRALLET
                     LHAQVLLEPRWRALDPEGRSLFDVDEPADLQG"
     gene            complement(1545..2516)
                     /locus_tag="VE646_11275"
     CDS             complement(1545..2516)
                     /locus_tag="VE646_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017608667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZD80605.1"
                     /translation="MMAPPKRLIDLEIDGEPARIPEGSTLLDACRARGIDTPTLCFLE
                     SLTPVNACRVCVVEVEGSRALVPACSRVVEPGMKVRTDSERVRHSRRMVLELLGSSVQ
                     LSLAGEDVRRWIGAYGATPERFGPPAEPSVPGEREGRHAGHHGPPAGAAAETVAQPVK
                     VDNDLYVRDYSRCILCYKCVEACGVDAQNTFAIAVAGRGFDARISTEFDSPLPESACV
                     YCGNCIGVCPTGALMFKSEHDMRRAGSWDESKQEVVTTICPYCGVGCNVELHVQDNEI
                     VKVTSPLDHSVTSGHLCIKGRFGYAFVQNRGNGADGDGAPERTAPEG"
     gene            complement(2531..4405)
                     /locus_tag="VE646_11280"
     CDS             complement(2531..4405)
                     /locus_tag="VE646_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HZD80606.1"
                     /translation="MDLRLMRAAPSEAERQAVDAVLGPPASGWRGGEWRPEREGRIAV
                     GGRRGADGHRHLLLPALHALQSRVGWIDEGGLNYVCERLSVPPAEAYGVATFYAMFSV
                     EPRPVRVAHVCDDIACRVRGARELRAELERSVGPPGSVEGEAAFTWMPSPCLGLCERA
                     PAVLFQTAGDPPRDWAIAPTRTSQVLDVLRGDPWTRDGEGVSGVAGGDRRLLRRVGVV
                     DATSLDDYRAHGGYESLRRAFRLGPGGVIQEVKDARLLGRGGAAFPTGVKWEAVAAQP
                     IQPHFFVCNADESEPGTFKDRIAMEEDPFAVVEALTIAGYATGAERGFIYLRGEYPLA
                     LRRLEHAVDRARARGFLGADVMGEGFAFDIEVRRGAGAYIAGEETALFNSIEGRRAEP
                     RNKPPFPVTHGLFGKPTGINNVETLLNVLEILRIGGPAFAGIGTEDSTGTRLFCLSGS
                     VRRPGLYEVPFGTTLRRLLEMAGGVGEERQLRAVLLGGAAGAFVTPEELDMPLTFEDA
                     RRLGVSLGSGVVMAFDDTVDLTDIVLRIAAFFRDESCGQCVPCRVGTVRQEELLHRLA
                     AGEADGSSTEQVALLADIARVMGDASICGLGQTAANAVRSAVERLGLFRQGDANGAGG
                     "
     gene            complement(4416..>5633)
                     /locus_tag="VE646_11285"
     CDS             complement(4416..>5633)
                     /locus_tag="VE646_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZD80607.1"
                     /translation="GVTEHHNAVDNVFALINLALLCGHVGRYGSGLNPLRGQNNVQGG
                     GDMGALPNKLPGFQDVADDELRAKFERVYGTSIPARPGWHVSAMLEAMERGELTALYC
                     IGENPAQSEADSHRTEKLLQGLDGLIVQDIMLTRTGEMADVVLPAAAAWAECEGTVTS
                     SERRVQRVRKALEPPGLARDDMWILGELSRRFGHDWGEVTAQAAWDELRSLSPIHAGM
                     SYPRLGALGGLRWPCRDEDDPGAMYLHGRLWEEGEERRGRRAPFSVVEHEPPVDELTP
                     EYPIRLTTGRRLDSYNTGVQSGQFVSPLRRRPESLVLSPEDARAYGLQDEEVVRVTSR
                     RGTVDAPVAIDRSLRPGLAFMNLHFQDLVATNVLTVDATDARSGTAEFKATAIRIEKL
                     LSPAAAAGGGGGE"
BASE COUNT          790 a         2177 c         1888 g          778 t
ORIGIN      
        1 ggttgatcac atggcggagg tcgatcggcg aggcgatcag gaccaggtcc accggcgccg
       61 cgtcgatcat cttctccatc tcgcgcaact gttggtccga gtaccccatc gccggcagga
      121 ccgggcccac gtcgtactta cggtacgtct cctcgatcgt tcccaccgcg tacggtcggg
      181 ggtcgacgat ctccgccgcg ccgttccggc gagccgccac caccccggcg ccgaacttca
      241 tctcgccgtg cgtcagcgtc gggccgtcct ccaccaccag gacccgcttg ccccgcacct
      301 gctcgccgtc ctcgagcgtc ccgaaccgct gcacccgctg ggcgttcagg tccccgtacg
      361 gcatcgggtg acggaccgcc accacccgct tgcccgcgtc gcggaggacc tgcgccacgg
      421 cgcgcgtcgt ctggctcttg ccggagccgg tccggaccgc gcacacggcg accaccggga
      481 cctgcgccgt gagcatggtc tgcctcggcc ccaggaggag gaagttcgcg ccggccgcca
      541 gcgcctcgct gcccttgtgc atcacgtact cgtgcgacac gtccgagtag gcgaacacca
      601 cgtcttggac cccgaggtcc cggatcagcg aggacagctc gctctcgtcc cggatgggga
      661 tcccctccgg gtagcgggga ccggccagct cggaggggta gcgccgatcg ctgatgaagg
      721 ggatctgggt ggcggtgaag gccaccacct cggagccctc gtcgtcccgg tagacggcgt
      781 tgaagttgtg gaagtcgcgc cctgcggcgc ccatgatgag aacacgacgc ggcatgccgg
      841 gtcctttccg agagaggggg tctgatgcgc gggcgggcgt ccgctcttca ccccggaatc
      901 tacccgaccc gcctgcggtg caccaccggg ccgcatctcc cggcggtcag ccctggaggt
      961 cggccggctc gtccacgtcg aacaggctcc ggccctcggg atcgagcgcc ctccatctcg
     1021 gctcgaggag cacctgcgca tgcagcgtct cgagcagggc ccggagccgc cgctccccgt
     1081 ccgccagcaa cgcggccgct gcctccaccg ccggcaccac ccgcagcgcg cacgggagcg
     1141 gtcggaaccg atcgccgtcc tccaggacga cggcgggcac gcccggatcc gccatctcct
     1201 cgagcatcgc gccgaggacc acggcggaca gctcgggcat gtcgcccccc gccacgagcg
     1261 cgagcgtcgt ccggcacgcg ggaagccccg cggcgacgcc ggccagcggc ccctggtccg
     1321 ccacgtcgtc ccgcgccaac cggatcggca ggtcggaagg gagcggcggc cgctgccccg
     1381 gcccgaccac caccaggacc tcgtcgcaga cctcggcgag ccgggagacc gtccactgca
     1441 ggatcgaggt gccgcggtag ggctcggcca gcttgtccct gccgaaccgt ctggatcgtc
     1501 ccccggcgag caccacgccg gtcacggatc ccgcggtccc caggctagcc ctccggtgcg
     1561 gtgcgctcgg gagcgccgtc gccatccgcg ccgttcccgc ggttctggac gaacgcgtac
     1621 ccgaaccggc ccttgatgca caggtgcccg ctggtgaccg agtggtccag gggggaggtg
     1681 accttcacga tctcgttgtc ctgcacatgg agctccacgt tgcatcccac gccgcagtag
     1741 gggcagatcg tggtcacgac ctcctgcttc gactcgtccc agctgccggc ccggcgcatg
     1801 tcgtgctcgc tcttgaacat cagggcgccg gtgggacaca ccccgatgca gttgccgcag
     1861 tacacgcagg ccgactcggg cagcggtgag tcgaactccg tggagatccg ggcgtcgaag
     1921 ccgcgaccgg ccaccgcgat ggcgaacgtg ttctgggcgt ccacgccgca ggcctccacg
     1981 cacttgtagc agaggatgca gcgcgagtag tcgcggacgt agaggtcgtt gtccaccttg
     2041 acgggctggg ccaccgtctc ggccgccgcc cccgccggtg ggccgtggtg accggcgtgt
     2101 cggccttccc gctcgccggg taccgatggc tcggcgggtg ggccgaaccg ctcgggggtc
     2161 gccccgtagg ccccgatcca ccggcggacg tcctcccccg ccagggacag ctggacggac
     2221 gagcccagca gctccaggac catccgtcgc gagtgccgga cccgctcgga gtcggtccgg
     2281 accttcatgc ccggctcgac cacccgggag cacgccggga ccagcgcccg ggagccttcc
     2341 acctcgacca cgcacacgcg gcatgcattc acgggggtca ggctctccag gaagcacagg
     2401 gtcggggtgt cgatccctcg tgcccgacag gcgtccagca gcgttgagcc ttccgggatc
     2461 cgggcgggct caccgtcgat ctcgaggtcg atcagccgct tcggcggagc catcatcacc
     2521 tgcgccacgt ctatcctccc gccccgttcg cgtccccttg ccggaacagg cccaggcgct
     2581 ccacggccga ccgtaccgcg ttcgccgcgg tctggcccag accgcagatc gacgcgtcgc
     2641 ccatgacccg ggcgatgtca gcgagcagcg ccacctgctc cgtgctcgag ccatcggcct
     2701 cgcccgcggc cagccgatgc agcagctcct cctggcgcac cgtgcccacc cggcaaggga
     2761 cgcactggcc acaggactcg tcccggaaga acgcggcgat ccgcagcacg atgtccgtca
     2821 ggtccaccgt gtcgtcgaag gccatcacca cccccgagcc caggctcacc cccagtcgcc
     2881 gtgcgtcctc gaaggtgagc ggcatgtcga gctcctcggg ggtgacgaag gcaccggccg
     2941 cgccgcccag cagcacggct cggagctgcc gctcctcgcc cacgcccccg gccatctcca
     3001 ggagccggcg cagggtcgtc ccgaagggca cctcgtacag gccgggccgc cggaccgatc
     3061 cggagaggca gaacagccgg gtgccggtgg agtcctcggt cccgatgccc gcgaaggccg
     3121 ggccgccgat ccgaaggatc tcgagcacgt tcagcagggt ctccacgttg ttgatgccgg
     3181 tgggcttgcc gaacagcccg tgggtcaccg ggaacggtgg cttgttccgg ggctcggccc
     3241 gtcggccctc gatcgagttg aacagggcgg tctcctcgcc ggcgatgtag gcgccggcac
     3301 cacgccggac ctcgatgtcg aaggcgaacc cctcccccat cacgtcggcc cccaggaagc
     3361 cccgagcccg agcacggtcc accgcgtgct ccagccggcg cagagccagc gggtactcgc
     3421 cgcgcaggta gatgaagccg cgctcggcac cggtcgcgta cccggcgatc gtgagggctt
     3481 cgaccaccgc gaaggggtcc tcctccatcg cgatccggtc cttgaaggta cccggctcgg
     3541 attcgtcggc gttgcagacg aagaagtgag gttggatcgg ctgggcggcc accgcctccc
     3601 atttcacgcc ggtggggaag gccgctccgc cccgccccag caggcgcgcg tccttcacct
     3661 cctggatcac gccccccggc cccagccgga aggcacggcg caggctctcg taccctccgt
     3721 gcgcccgata atcgtccagg ctggtggcat cgaccacccc cacccgccgg agcagtcgcc
     3781 ggtccccgcc cgccaccccg gagacgccct cgccgtcacg ggtccaggga tcgccgcgca
     3841 agacgtccag gacctgcgag gttcgcgtcg gggcgatggc ccagtcccgc ggcggatctc
     3901 cggccgtctg gaacagcacc gccggcgctc gctcgcacag acccaggcac gggctcggca
     3961 tccaggtgaa cgcggcctca ccctcgacgc tccccggcgg gcccaccgag cgctccagct
     4021 cggcgcggag ctcgcgcgcg ccccggaccc ggcaggcgat gtcgtcgcag acgtgggcca
     4081 cccgcacggg ccgcggttcc accgagaaca tcgcgtagaa ggtggccacg ccgtaggcct
     4141 cggcgggggg gacggacaaa cgctcgcaga cgtagttcag gccgccctcg tcgatccatc
     4201 ccacgcgcga ctgcagcgcg tgcagggccg gcagcaggag gtgtcggtgg ccgtcggcgc
     4261 cccgccgccc tccgacggcg atccgaccct cgcgctcggg gcgccactcc cctccccgcc
     4321 acccggatgc cggtgggccg agcacggcat ccaccgcctg ccgctcggcc tcggaaggag
     4381 cggcccgcat caaccggagg tccatcgccg ccccctcact ccccgccgcc cccgcccgcc
     4441 gccgcggcgg gactgaggag cttctcgatc cggatcgcgg tggccttgaa ctcggccgtg
     4501 ccggatctgg cgtcggtggc gtccaccgtg agcacgttgg tggcgaccag gtcctggaag
     4561 tgcaggttca tgaaggccag tcccgggcgg agggaccgat cgatcgccac cggcgcatcc
     4621 accgtcccgc gtcgagaggt cacccggacc acttcctcgt cctgcagccc gtacgcccgg
     4681 gcgtcctcgg gcgacaggac cagggactcc ggccgccggc ggagcggcga gacgaactgg
     4741 ccgctctgca cgcccgtgtt gtaggaatcg agccggcgcc cggtggtgag cctgatgggg
     4801 tactcggggg tgagctcgtc caccggtggc tcgtgctcga ccacgctgaa cggcgcccgc
     4861 cgcccccgac gctcctcgcc ctcctcccac aggcgcccgt gcaggtacat cgccccgggg
     4921 tcgtcctcgt cgcggcacgg ccaccgcaga ccgcccagcg cccccaggcg cggatagctc
     4981 atgccggcgt ggatcggcga aaggctccgg agctcgtccc acgcggcctg cgccgtgacc
     5041 tcgccccagt cgtggccgaa ccgccgagac agctccccca ggatccacat atcgtccctg
     5101 gccaggccgg gcggctcgag ggccttgcgc acgcgctgga cgcgccgctc gctgctggtc
     5161 acggtgccct cgcactcggc ccaggcggcc gcggccggga gcaccacgtc ggccatctcg
     5221 ccggtcctcg tgagcatgat gtcctgcacg atcaggccat ccaggccctg cagcagcttc
     5281 tcggtgcggt gggagtccgc ctccgactgc gcggggttct ccccgatgca gtacagggcc
     5341 gtgagctctc cgcgctccat cgcctcgagc atcgccgaca cgtgccatcc cggccgggcc
     5401 gggatggacg ttccgtagac ccgctcgaac ttggcccgca gctcgtcgtc ggccacgtcc
     5461 tggaagccgg gaagcttgtt gggcagggcg cccatgtcgc cgccgccctg cacgttgttc
     5521 tgaccccgga gggggttgag acccgacccg taccggccga cgtgcccgca caggagcgcc
     5581 aggttgatca gcgcgaacac gttgtcgacg gcgttgtggt gctcggtgac gcc
//
LOCUS       DASQQQ010000299        28946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_19589, whole genome shotgun sequence.
ACCESSION   DASQQQ010000299 DASQQQ010000000
VERSION     DASQQQ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 28946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28946
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19589"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VE646_11290"
     CDS             <1..241
                     /locus_tag="VE646_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogen fixation protein NifH"
                     /protein_id="HZD80608.1"
                     /translation="SGYVADVLQNLELLAELGHARDPRLANALELVLSKQDRSGRWRN
                     ENAYERTTWVPFEKVRAPSKWVTLRACRVLKAALG"
     gene            complement(892..2082)
                     /locus_tag="VE646_11295"
     CDS             complement(892..2082)
                     /locus_tag="VE646_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80609.1"
                     /translation="MPDLDQRLRSFDGIRAPELWERIERHRPGPEAPEPTRSLALRVG
                     VAFLALLVAAVGLAAITRGFLHPDTRPASPSPVPRITATIHLDAHPAGVVAARGVIWV
                     SNGDDGSLTPIDPTTNRPGPAVLCSMGSPLVADGALWTVGNNGNLCLPGGARAAQMLF
                     RLDPATGAVQAETTLQTDPRLYATKVAAGAGALWAASWGEPGTGDHGRFRGVLSRVDP
                     SSGRVTARRRIGIWMGGLAAGFGGVWVADAPGHRPGTLLRIDPGTLAVVARIPVGIGP
                     VDVTTGYGSVWVANDADGTLMRIDPTSNTVVASIRVPGVGALAVGQGGIWCTSFAGLV
                     TLIDPATNQVAGTPLRIGRKLELTGITVGDGAVWVTRAVWEMNASDQTVTRIALSTST
                     SPAP"
     gene            complement(2075..2545)
                     /locus_tag="VE646_11300"
     CDS             complement(2075..2545)
                     /locus_tag="VE646_11300"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HZD80610.1"
                     /translation="MLEVSRSSTTIETIYRDDAARLWRALYAFGGDPEIASDALVEAF
                     AQAIRRGEAITSPAAWIWRAAFRIASGELADRARFLPLEQDQRAAPEGADLDLVAALG
                     RLSPNQRASVVLHHIGGYSTRETARIIGSTAGAVRVHLSMARRRLTQLLEEDDA"
     gene            complement(2703..3767)
                     /locus_tag="VE646_11305"
     CDS             complement(2703..3767)
                     /locus_tag="VE646_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZD80611.1"
                     /translation="MLTNADLGELLWTAGREETDHRRRALEKASRASRFWPEEASEVA
                     RAGRSLTELRAVGPWVAARIHGWLEDPPPVPQPDETRRGFLTLAEVRRTLDADRAWEA
                     TPCADLQMHTTWSDGTLPLEEMVQSARALARSYVAVTDHSQALAIAHGMTAEQLAEQG
                     RLIDELNRAFGAEGDPFRILRSMEVDLFPDGTLDMDDATLRTLDLVLGAFHSKLRSKE
                     DETERYLAALRQPRLHILAHPKARMFGRRAGLSADWRRVFAEAARLGKALELDATVWR
                     QDLGVELATIAREEGVAWFSIGSDAHTALELEFLPFGMATAALAGIPRESILNYRPAE
                     FVLSWASGNTVGRPGPRSSG"
     gene            complement(3813..4250)
                     /locus_tag="VE646_11310"
     CDS             complement(3813..4250)
                     /locus_tag="VE646_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014926943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZD80612.1"
                     /translation="MATRIQIVNDCADPSGLAAFWAEALHYRLQDPPEGASSWEDWLR
                     EQGIPEERWNDASAVVDPDGAGPRIFFQRVPEGKVVKNRVHLDLSISGGIRAPLEERQ
                     RSVDGEVERLQGLGATIFRPGSVQRGEYWVVMQDPEGNEFCVQ"
     gene            4739..5683
                     /locus_tag="VE646_11315"
     CDS             4739..5683
                     /locus_tag="VE646_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80613.1"
                     /translation="MSGRVCTDRAPYRHTVTAAYDPATDSWRMLPPYRGPKGCFEGAD
                     HALWDGHEMLLLGVTNAAYDPSTNSWRRLPGWGGGGGEVNAWTGRQTLTFGGGCCGDQ
                     EGMGSSYVARTNRWTQPPAGPLVGRHGVVGVWDGHGFVVAGGYHEVEETTENGTPVRL
                     GPRILLDAAAYDPSTRAWRSLAPMPRLPGVHHVWSRTAVWDGSEMLVVSAFGNLAYDP
                     ATDRWRLLPDMRFQRGGAGAVWTGTRMLVWGGATNEGEGAPPYGEAYDPAIDRWLSMP
                     PSPLRHRYPAVVVWTGTQMIIWGGDMFPDGATFTPVGS"
     gene            complement(5806..6594)
                     /locus_tag="VE646_11320"
     CDS             complement(5806..6594)
                     /locus_tag="VE646_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HZD80614.1"
                     /translation="MRRLWLVVALVATLLQIPHPAAAGTVTVAAAGDIARPTLSTPQQ
                     QTADLVTAFDPTTVFALGDLQYENGELANFQSSYDPSWGAFKSKTHPIPGNHDYGTTN
                     AAGYYTYWGSAAGDPSKGYYSFDLGDWHVVALNTECTQINCSAEKSWMKSDLAADGHL
                     CQMVVYHRANLKWPRKKAEAAGVDLALTASRHVYERWAPENGLLRLIVGTGGYSLGKL
                     NSGAVVGIRAYGVVELTLSSSSYSWSFVDTSGDVRDSGAGSCHS"
     gene            6772..7791
                     /locus_tag="VE646_11325"
     CDS             6772..7791
                     /locus_tag="VE646_11325"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HZD80615.1"
                     /translation="MGARLGLTRSQILAFRRRAGALDERLHRGAGSLRRAAWAGLQDS
                     MPRAALLSIHARVRGTHPTTWEDPSLVQLWGPRYSAYVVAARDRAIFSLGRLPEDDRA
                     RGVAEELAARLDALLDGARMPYEQAGRALGVHHNRLRYAAPTGTVLIRWEGARQPVIW
                     TVPPPQIDPRDARLELARRYLHVFGPATPETFARWAGIAPREAIAAFHALGRSLASVR
                     TPIGEGWVLARDVPTFRAAPGPAAPARLLPSGDAYFLLQGADRELLVPHRERRGALWT
                     PRVWPGALVVDGEVAGTWRRAHQTLTIQVWRRLTRAERDAVEAEAASLPLPGIGGEIV
                     VRWEV"
     gene            7944..9419
                     /locus_tag="VE646_11330"
     CDS             7944..9419
                     /locus_tag="VE646_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD80616.1"
                     /translation="MASDARTAHDVVETDIPARMDRLPWSRWHWLVVVALGITWVLDG
                     LEVTIVGAIGAILTNKHTLALSDAEVGLAGAVYVAGNVVGAFLFGYLTDRLGRKRLFT
                     WTLGLYLAATVATAFSWNVMSFFVFRFITGMGIGGEYSAINSATEELNPARVRGWVEL
                     GINGSWWVGTAVGAASSILLLNKSLLPVDIGWRVAFGLGALLGIGVLLIRRLLPESPR
                     WLMTHGRVQEAEGVVADIEDDVRRYTGREQLPDVEGTTIKIRPRERTTIREVLRSMFV
                     ERRRRVVLGLGLMVGQTFLYNAVFFTFAITLDTFFKVPAERVGWYILPFAVGNFLGPL
                     TIGRLFDTVGRRAMISTTYVLSAIGVAITGYLFTTGTLGAWGLTMALSFIFFLASAGA
                     SAGYLTVSEIFPLEIRALAIAFFYAIGSAIGGVAGPYLFGALVETGKEANLFIGYALG
                     AGLMLLGGVMEAFMGVDAEQRSLEEVAEPLSSEGVERGDAA"
     gene            complement(9426..9671)
                     /locus_tag="VE646_11335"
     CDS             complement(9426..9671)
                     /locus_tag="VE646_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80617.1"
                     /translation="MEDQRFVVVASDLAEIDDIAHAPDVLVLDRLDVVVLLRTTPERS
                     RELSSRPGAVVSVYATESLARMAFEPFRHRPGRPASW"
     gene            complement(9828..10766)
                     /locus_tag="VE646_11340"
     CDS             complement(9828..10766)
                     /locus_tag="VE646_11340"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HZD80618.1"
                     /translation="MGVGVAAFSQRHLFEGFASIVTRLTWYWVGLAFIVEMGSVPPLA
                     EAELIVLRAGGVHADRWRMNLVTLASNAIAMSIPAGVAVAEGYTYSRYRQLGAEPAAA
                     AWAELASGALAFAALAAVALAGGVLAGGPDSTVLVPTLAVVTVGSVGAAALFRHPHLL
                     AAGLGWVERTFGRDRGSLVARTTQRIGRTAGQLSFPHPDVATWLTAFGLSLANWLMDV
                     AALAFAFLAAGGHVPWGVVLLAFAGAKVVSSIGITPGGLGIVEGGMVATFVAYGVQGP
                     TAVAAVLVYRGLTYLGLVGIGWAAVGLLSLQRGRRG"
     gene            complement(10827..11144)
                     /locus_tag="VE646_11345"
     CDS             complement(10827..11144)
                     /locus_tag="VE646_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80619.1"
                     /translation="MRAYCPKTLFLFSSHTAPAEGCSCGIYASKSQPTYDRPDRVRTD
                     YYGKTSLWGVVRLTGKVIEHSGGFRAEYARPVALVMNEDTPLDIVDSYGLLPIGSLTD
                     WKL"
     gene            11612..13381
                     /locus_tag="VE646_11350"
     CDS             11612..13381
                     /locus_tag="VE646_11350"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HZD80620.1"
                     /translation="MDPQGPVGGPRPAPPPPPSALRAEPPRTASPRSARRGIRVLAVV
                     LASLLVVAGTGAAAAFYLARGASEELIQMIPASSDVVVTAYLDPSAGQKLNLLSLMHR
                     FPALADDRQLRGRIDDLLDQALEDAGLSHQDVRSWLGSEVAVAIELQPGLGDPTTAIL
                     ITSTDDDAAETAIEQGLRTSLGAGGSRGYRGVSLHTYGAGSSAVTFGIVDHVVVLGSR
                     ADGVTRVIDVAKGTGRAIGDDPRFSRTISTLPRDRLGFAYVNAGGIVRASLEANGLGA
                     AIGAGTGLDTLRALEGIGVSLSAHPDGLALDYTIGVDPSKLQPEARAQLEQPSGQNGV
                     VRLVPGDALAVMTQRGLAAGLRAAVDQALATPEGERVRQRLGVDQMLAALTGDVAVEV
                     APEAPATPIGGAVVLGVSDAKAVQRTLDGLARLAIRAQRRAEGAVPTPFGDLTGTVVL
                     AKSFTPEAPTVRLRTTTYRGVRIRYVEDPSLSAMSGVLPAYAVVDGAAIIGSTPMDVR
                     KVIDVRDGIRASIVDSPSYLDALAHVPGDGTLYVDVAGVVSGLGPQLPPDVRWNLEPL
                     RAVVLGSTSSPSRITGRLFVRIG"
     gene            13462..13725
                     /locus_tag="VE646_11355"
     CDS             13462..13725
                     /locus_tag="VE646_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80621.1"
                     /translation="MRGDRIEVVVDSGNGVLRYEIEATRAGRRVEVSTSRSVIEVTEV
                     TRTGAPVRSGRFMADRVVALVEHPAPPPPTRGRRPPRGQVSLV"
     gene            13725..14003
                     /locus_tag="VE646_11360"
     CDS             13725..14003
                     /locus_tag="VE646_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80622.1"
                     /translation="MDDEALLALAEKTLTEIKIAQGLSDEHASVLAALRIRLKGSAGG
                     SLEELLQAAGDLGGESLEDKLAEAERRPKPSLDDLLKQDPKPKRDWPS"
     gene            14057..15784
                     /locus_tag="VE646_11365"
     CDS             14057..15784
                     /locus_tag="VE646_11365"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZD80623.1"
                     /translation="MDLRSPRPVIAAASLGLSLLLTACAAVSSGPAPAASGSGAASAA
                     PSASLSGTSVLSAADAQLADQVCTQNSPEVLLRTYRGTQLGRSGDIQLIPRYPDFMGA
                     GLPHATPYAYTQDVPLFLYGPGYVKPGTYTRPASLTDIAPTEGAILQFPFHAPDGVAQ
                     TEALLPSSGRPPPRLVVTLVWDSGGMDVLDAWKRDWSYLRSLLPHGAWFTHATVGASP
                     SNTPTGHAEIGTGAFPVHNGFVDDYIELDGRIQKPNANGPAFLEVPTLADLYDRAMGN
                     RPVVGAVVTLSAHVMMMSHGSLWGGGDRDIAITREVTNASTAGAEAGRWNLSSQMAPY
                     YRIPAYVNSLPPLAHYLRPIDALDGQLDGAWRGNSIAQLQDGFDTPARTPYQTKLIEA
                     VIQREGFGKDDVPDLLSLNYKAIDVVGHLFSANSVEMSDTLRIQDQALRQLVGFLNAT
                     VGKGRWVMVLTADHGTQRLPTVSGAFVANIDRFTADIEHRFDHDGDGTPLLLRVRPTQ
                     LWVDPAEMARNHVTLDQISQYVMQLTQSQLTRSGVQADPATANDRVFSGALPSRLLRS
                     LPCLPPNVG"
     gene            15888..17009
                     /locus_tag="VE646_11370"
     CDS             15888..17009
                     /locus_tag="VE646_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014289325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04053 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HZD80624.1"
                     /translation="MDFNQAPILLFWETTRACRLACRHCRAEAIATPLPGQLSTSEGI
                     RLIEDLRGFRPHRPVLIFTGGDPFMREDLWELADRARGNDLPIGFAPSVTPLLTERAA
                     RRMREAGARTVSISLDGADAASHEGVRGVGDHFARTEEAVRMLVREGLSVQINTTVMR
                     RNVEQLADIAGLVAAWGADIWEVFFLIRVGRGSELEELTPLENEDVVQFLYEASRYGF
                     IVRTVEAPFFRRVVASRRELDPSADPAAAFGLGPMYRYLAERLRSTLGTPGPSRAQGV
                     GTRDGKGILFVAHDGDVYPAGFLPVRLGNVRQDDVVALYRHHPMLRAIRGAAFSGRCG
                     RCRYADLCGGSRSRAFAATGDPLGEDPACAYEPGPALVT"
     gene            complement(17018..18154)
                     /locus_tag="VE646_11375"
     CDS             complement(17018..18154)
                     /locus_tag="VE646_11375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HZD80625.1"
                     /translation="MRIGAPGHGSLLLRGRYPHAASRCVRPPSRSFRARYPGNLRIVR
                     SEDGTLSLTLTLPFERYLEGIAEVPPSWPTAALQAQAIAARSYALATTGWSGSQGQTL
                     RTPICATTSCQVYRGIPVPPQPDVRRWYRAVRSTSGLVLLYQGRPAETVYFSTSNGHT
                     YGNEQVFGSAPLPYLRPVVERDDGASPTSHWRVRIPFSDLTRFLREGGTWTAHRRVAR
                     IQSQGSTIVLSAGQSSRSLDLDSFRGIVDAWAPCLEPAHYPTDSLPVTIPSHWVRVSS
                     TRGAAVFTGRGWGHGVGMVQWGAYGKARRGFSASQILAYYYGGLRPQPYPEPGLIHVR
                     VASGLASLRVTPSGPGGRLDGSPLEPGRRVIVTGGDRLQVSTVP"
     gene            18690..20153
                     /locus_tag="VE646_11380"
     CDS             18690..20153
                     /locus_tag="VE646_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020639773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HZD80626.1"
                     /translation="MATEASYPLRVEAELEPRLSRWLWLVKWFLAIPHFIVLGFLWIA
                     FSVLTLVAFFAILFTGRYPRSIFEFNVGVMRWTWRVSDYAYGVLGTDAYPPFTLEPVP
                     GYPARLEVAYPEHLSRGLVLVKWFLAIPHLIIVGILASGGTWLAWNADHLNWGAGGGL
                     IGLLVLVAAVILAVTGTYPRQLFDLILGLNRWTLRVAAYVSLMTDRYPPFRLDMGGNE
                     PGGVGTLTIPAPPSSEPGPTLPAPPPSGGARRGGWTGGRITAVAIGSLLALVSFALFA
                     GGGFLLWAFGTQRDAQGFYSTPERTYQANTYALITRARVTEPGPGWFSIRGGGWLFGA
                     VSEEAIRIRVNEQGGGNVFVGVAPSTDVDRYLSGVARTVVTDLPDPDYRSLSGSAPAG
                     APSSQAFWEASATGAGTRSLRWDLHEGSWTIVVMNADGSRGIDIRADAGARVPALPWI
                     AAGLLIGGAVVLAGAALLIAIPVSRANRSTRRGGSMP"
     gene            complement(20181..21644)
                     /locus_tag="VE646_11385"
     CDS             complement(20181..21644)
                     /locus_tag="VE646_11385"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD80627.1"
                     /translation="MPDAPPPGYGAVTTNVVLALSPFLVVTTGFVFLSPVIAHHLGTS
                     RTTIQVFDGLAIAGYAFGALLGGDIVQRFSQRTLFVICESGFVVGSLTAAVATEPIAF
                     GAGHVLQGFATGLLLVIAVPPLVNRYPARRMRTTAAFIDIGFFGAVTAGPLLGGPAAA
                     AGHWRWFLGGVALVGAANLALGLVVLQHTEPMNPGFPMDRSALSLALVATVLPFLATS
                     WVDPTPFSSPLFIVPLTVGLTALVVLLFSQYHKREPLSPVRTMSNTIPVAGTIVAAVA
                     GGAFVSFLELGQQYLLTTVHESPTHVGLLYWPMVLGTIVASVALAMLLRTRFLPHLVL
                     AGMTCIAAGGAVLFAGRGGGDIVILISAALLGTGAGATVSPALWMAAFALPAMMVGRT
                     FALVELVRSVADFVLAPVILAIAVTIGTAGSRWVGVATALWVTLGVTIGGLGVIVGLY
                     LVGRPPSRAPDLESWLEGESPALASPRIGAVVRDDAA"
     gene            21808..22035
                     /locus_tag="VE646_11390"
     CDS             21808..22035
                     /locus_tag="VE646_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80628.1"
                     /translation="MRTFTDQQRYVVVLGDAADEVSEAPTVTVLDRLDRVVLLEASYR
                     TAVELRHRDGVIIHIYERERDARRAFGLFVH"
     gene            complement(22076..22753)
                     /locus_tag="VE646_11395"
     CDS             complement(22076..22753)
                     /locus_tag="VE646_11395"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZD80629.1"
                     /translation="MTGPPAMLFDLDGTLVDSVYQHVLAWREALEAEGIQLSVWRIHR
                     RIGMSGGLFAQALLRETGVPLSPQIQDRLQRGHAQAYARLADRVQPLPGARELLRKLT
                     ELDVAWAVATSGHLESAGPTLDALEVPEGVPVITRDLVERAKPDPDLFLEAALRLGVD
                     ISNAVVVGDSVWDLLAARRARSLGIGLLSGGYGMEELERAGAYRVYQDPADLLRHLDE
                     VGVRDAG"
     gene            complement(22750..23067)
                     /locus_tag="VE646_11400"
     CDS             complement(22750..23067)
                     /locus_tag="VE646_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80630.1"
                     /translation="MPEHDCLLCRAEHVTAWHFEDEDCWVADCLVCATPMVVWRTHGL
                     PDPEEEARLLAHLDRVAAQRYGEEGYWIDPERRRIPDHWHAHARPAGGFFDPHSDLYG
                     RYG"
     gene            complement(23060..23521)
                     /locus_tag="VE646_11405"
     CDS             complement(23060..23521)
                     /locus_tag="VE646_11405"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HZD80631.1"
                     /translation="MLALLVVLASLVTACSGGGRPAAATTPADRVRIRTAGGTVTLRV
                     EIADTPAERERGLMGRTSLPADQGMAFVWDQPVRARFWMKDTLIPLSIAFWDHDGRIV
                     ALREMVPCRREPCRTYDAGAPFVGAVEANRGFFAHHGVAVGDVVELVRSDA"
     gene            23585..23657
                     /locus_tag="VE646_11410"
     tRNA            23585..23657
                     /locus_tag="VE646_11410"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23618..23620,aa:Thr,seq:ggt)
     gene            23855..24319
                     /locus_tag="VE646_11415"
     CDS             23855..24319
                     /locus_tag="VE646_11415"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZD80632.1"
                     /translation="MLDEGSRRRLEGAYREYGAEVWRAIYAYSGGSRHIADEAVAEAF
                     AQAGRGLDRIRDLRPWLFRAAFKIAAGELKQRPAPSLRPPAPQDGQGMMELLDLARSL
                     TSAQRRAFALRDVLGFSSREAAKLMGTSEVAVRVHLHAAHRRLRALVLEAES"
     gene            24316..26121
                     /locus_tag="VE646_11420"
     CDS             24316..26121
                     /locus_tag="VE646_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80633.1"
                     /translation="MRIEDLRRLRSEEPLDLWPEIERRLSLPSPGGRSPLRRVSTLVA
                     AVGIAFIFGLSLYALTGLIGGRGASVGSGGIVRVPVPAPQPLAAGEGAVWVVGGLSDQ
                     GGLWRVDPTTDRARLVAGTEGAWWPATGEGAAWVTTCRPGDKTDCARADLLRIDPASG
                     TLTASLRLPAPPMQIAAGLGSVWISADQHLLKIDPVSMSIVGEIPLHTNLLGTAGGFL
                     WATVGGAGATGVDKIDPSTGRVLAHIPFGDPCTFAASGDLVAVASCQGGLPPGSGPDH
                     LVGIDPSTATVLYDVPLSSYGGLAFAAGYPWIASWTKDPERVEVRQLDASTGLPTGVS
                     VSLGPGPRPWMELGMGAPGVSVATSDGSFWLTHVDADDVVRVDVPVADASRSSSSAGA
                     AQGRCVKVGAQGDFDDDGTPDTVRLYDEVAQGQTCHQDMSHLLHLSAQFGSGGGFDIP
                     FSYCQGGACSRVLQAVDLDGDGRSELVIEVGPGAAIDSVELFRVSADGIIPLRVAPPG
                     DPGFVEPGPAILGGSFDSGGMSPVSCRSQPDGTTQIVSVHAAATGDLNTAPWDVHRTV
                     LALQGDTFGVVGSSDQTVEHFQPSAPVFSCAPGSP"
     gene            26239..26754
                     /locus_tag="VE646_11425"
     CDS             26239..26754
                     /locus_tag="VE646_11425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZD80634.1"
                     /translation="MYRMLEQQAGRRTVVEFDSFYREHYARLVRALLVLTSDPAEAED
                     LAQEALSRLYERWDRVQEMDSPQGYLYRTALNLNRKRVRRLLVRTRRAITLRASAPED
                     LPAAEDRIALQAAVARLPRAQREAAVLVELLGLDGNEAARVLRISPGAVRSRLLQARR
                     NLRAHLGGPDA"
     gene            26747..27934
                     /locus_tag="VE646_11430"
     CDS             26747..27934
                     /locus_tag="VE646_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80635.1"
                     /translation="MPEDLRDLLRRTVEWYEPRPPDPTDAPRRTRARARRGKVAVVLL
                     TFVLAAASFGLVFATFAGRNNPADRPPAPAPSPAYTRQIQGQLSVLQAKLADVHGQLA
                     SAAGLVGRLADRYGEVVRALQAAPNPTQAMRLRADRLQAQLATAKARTKELTAQEQAL
                     VKQIKALVARLQEGPSPRSSAQPEAAIDVGLARRICNVQRMSGDVNGDGSVDTVWTGS
                     LVGAEGSCPTEPSRHRVLVIDLDRDGRADVQSTAMDCRTWCVVYALPDLNGDGTAEIL
                     VNEGHLAYPASALIGVYELVGRDLVPIRFRDGSNRFTLSDSWQGFAGAYCSSSTFTTW
                     DGWTDGRGALRRVAYRTYAVNISSLGAWRFELVSSRTERTKERPSRTGWASLCGSPTT
                     SFG"
     gene            complement(28146..>28946)
                     /locus_tag="VE646_11435"
     CDS             complement(28146..>28946)
                     /locus_tag="VE646_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80636.1"
                     /translation="TLSNAFARPLAGGSRIRLNAQNTQGFTGGFDPGTDTVIYQQIDL
                     SARTNPSDLYFYDLDTQTRTSITEVNTRRWEWGPRISSSYILYNLDYRKNQVWRTAIE
                     LYDRSAGTRTRLTSWRTGKFNPPTGAVGETYATWTLCSSSCSAYVYDIATATTSEIPT
                     RKDRPQYAPVVDETNASVYYVRSATMSCGRKVVILRVPVLDPSQTPTRVLAMPDGIDI
                     DATMSLAPDPSTGGSDLLFSRVVCRNLNEDVYELADVGSLSDGSIHGS"
BASE COUNT         4134 a        10205 c        10383 g         4224 t
ORIGIN      
        1 ctccggctac gtcgccgacg tgctgcagaa cctcgagctg ctggccgagc tcggccacgc
       61 gcgggatccg cggctggcga acgccctcga gctcgtgctg tccaagcagg accggagcgg
      121 tcgctggagg aacgagaacg cgtacgagcg caccacctgg gtgcccttcg agaaggtgcg
      181 ggcgccgagc aagtgggtca ccttgcgcgc gtgccgcgtc ctgaaggccg cgctcggctg
      241 aggctctttc ccatcatcgc tcgaggatgg agtgagccgt gtgcgacagg tccgtccaca
      301 ggatgaaccc tgagcggtca gctcaacgcc gaccctcgaa gatcgaccgg cgtccgatac
      361 gcaggtcatc ctcgacaggt tcgagatcta gatcggcggg ttgccgtgcc agtcaagacc
      421 cgtgcagctc agatgctctc cgacgagtcc ggccacctct ctccccttga tcggcaggac
      481 ggatgtcgcc cgcaccgtcc tcgtcgagtg cgttgggacg tcgactccgc cgatgcccat
      541 gactggcacg aagaagggtt gggcccggtc catgctctga cactgctgaa cgctcgcgtc
      601 gcccccggtg ggttcgtgac ccgcacccgg taccggatcg agcacccgtc ggaggagcat
      661 ggctgccctg tcgcacgcgc ggcgatcacg atccctggga acggcgtcgg aaggatctcg
      721 gcctcgtgaa cccccggatg ggcggtacac gcgccggtga gcaccacgag cgtcaccaag
      781 gccaggcgct gcctcatctc aggagatgct ggcaccgtac cggccaccgg acaaaggtcg
      841 tgaccacgct ggctcgcgca ggacgccccg ggcggcccct gcaggtgccc atcagggcgc
      901 cggtgaggtg gatgtcgata gggcgatcct ggtcacggtc tggtcggatg cgttcatctc
      961 ccacactgct ctcgtcaccc acaccgctcc gtcccccacg gtgatgccgg tgagctcgag
     1021 cttcctgccg atccggagcg gcgtacccgc cacctggttc gtggccggat cgatcaaggt
     1081 gaccagccca gcgaagctgg tgcaccagat gccgccctga ccgacggcaa gggcgcccac
     1141 cccggggacc cggatactgg ccaccacggt gttggaagta gggtcgatcc gcatcagggt
     1201 gccgtcggca tcgttcgcca cccataccga cccgtagccg gtcgtcacgt ccacggggcc
     1261 gatccccacg gggatccggg cgaccacggc cagcgtgccc ggatcgatcc tgagcagggt
     1321 gcccggacgg tggcccgggg catcggccac ccacactccg ccgaacccgg cggccagccc
     1381 tcccatccag atgccgatcc ggcgccgtgc tgtcaccctg ccggaggagg gatccactcg
     1441 ggatagcacc ccgcggaacc ggccgtggtc gccggttccg ggctcgcccc agctagccgc
     1501 ccatagggcg ccggctccgg cggccacctt ggttgcgtac agacgggggt cggtctgcag
     1561 cgtcgtctcg gcttgaaccg ctcccgtggc cgggtccagc cggaacagca tctgggccgc
     1621 tcgtgcccca ccgggcaagc acaggttgcc gttgttcccg acggtccaga gggcgccgtc
     1681 cgccaccagc ggggagccca tgctgcacaa gaccgctggc cccggccggt tcgtggtcgg
     1741 gtcgatcggg gtcagcgagc cgtcatcgcc gttggagacc cagatgactc cccgggcggc
     1801 gacgacgccg gcgggatgcg catcgaggtg gatcgtggcg gtgatccgtg ggacggggga
     1861 cggtgatgcg gggcgggtat ccgggtgcaa gaagccccgc gtgatggccg ccagacccac
     1921 cgcggccacc agcagcgcca ggaacgcgac gccgacccgc agcgcgagcg accgggtcgg
     1981 ctcgggcgcc tccggtcccg gtcggtgccg ctcgatccgc tcccacaact cgggcgctcg
     2041 gatcccatcg aacgatcgca ggcgttggtc caggtcaggc atcgtcctcc tccaacagct
     2101 gcgtcagccg gcggcgggcc atcgagagat gcacgcggac cgccccggcg gtggacccga
     2161 tgatccgagc ggtctcccgc gtcgagtagc cgccgatgtg atggagcacc accgaggctc
     2221 gctggttcgg ggagagacgc cccagcgcgg cgaccaggtc caggtccgca ccttctggcg
     2281 cggcgcgctg atcctgctcc aggggcagga accgggcccg gtcagccagc tctcccgacg
     2341 cgatccggaa ggcggcccgc cagatccagg ccgctgggga ggtgatcgcc tcgccccgtc
     2401 ggatcgcctg agcgaatgct tccaccagcg catcgctcgc gatctccgga tcaccgccga
     2461 aggcgtacag ggcccgccat agccgcgcag cgtcatcgcg gtagatcgtc tcgatcgtgg
     2521 tgctggaacg cgagacctcg agcatgctcc ctccgccacc gtaataggaa gcgatgggac
     2581 atgggatgtt aaggacgatt gtcctccatc ttcggggcgg gctggaccgg ggggtttggg
     2641 cgatccgggg atcggctcgg gggtctagcc tggcggagct ggcgcgcccc gacggcggtc
     2701 agttacccgg aggaccgtgg ccctggtcgt cctacggtgt tgccgctcgc ccacgagagg
     2761 acgaactcgg ccggacggta gttcaagatg ctctcgcgcg ggatcccggc cagcgcggcc
     2821 gtcgccatgc cgaacggcag gaactcgagc tcgagcgccg tgtgcgcgtc gctcccgatc
     2881 gagaaccacg ccacaccctc ctcgcgcgcg atcgtcgcga gctcaacgcc gaggtcctgc
     2941 cgccacaccg tcgcgtccag ctccagcgcc ttgcccagcc gggccgcctc ggcgaacacg
     3001 cggcgccagt cggccgagag gccggcgcgc cgtccgaaca tccgcgcctt cggatgggcg
     3061 aggatgtgca accggggctg gcgcaacgcc gccaggtagc gctcggtctc gtcctccttc
     3121 gagcggagct tcgaatggaa cgcgcccagc acgaggtcga gcgtgcgcaa ggtcgcgtcg
     3181 tccatgtcca gcgtcccgtc cgggaacagg tcgacctcca tcgaccgcag gatccggaac
     3241 gggtctccct cggctccgaa cgcgcggttc aactcgtcga tgagccgacc ctgctcggcc
     3301 agctgctcgg ccgtcatccc gtgggcgatc gccagggcct gcgagtgatc ggtgaccgcg
     3361 acgtacgagc gtgccagggc ccgggcgctc tggaccatct cctcaagagg cagggtgccg
     3421 tcgctccacg tggtgtgcat ctggaggtcg gcgcacggcg tggcctccca cgcacggtcg
     3481 gcgtcgaggg tgcgacgaac ctccgccaac gtgaggaacc cacgacgtgt ctcgtcgggt
     3541 tgggggaccg gcggcgggtc ctcgagccat ccgtggatcc gggcggcgac ccatggcccc
     3601 acggcacgga gctcggtcag ggaacggccg gcgcgggcga cctcggaagc ctcctccggc
     3661 cagaaacggg aggcacgcga agccttctcc agcgcgcgcc gccgatgatc cgtctcctcc
     3721 cggccggccg tccacagcag ttccccgagg tcggcgttcg tcagcatgcg ctcaacctgc
     3781 caccggatcg gccggaccga gcgatccccc gctcactgca cgcagaactc gttgccctct
     3841 ggatcctgca tcacgaccca gtactcgccg cgttggacgg aaccgggacg gaagatcgtc
     3901 gcccccagcc cctgcagccg ttccacctcg ccgtcgaccg aacgctgccg ttcctccagc
     3961 ggcgcacgga tgccaccgct tatgctcagg tccaggtgca cgcggttctt caccaccttg
     4021 ccctccggca cccgctggaa gaagatgcgc ggcccggcgc cgtcgggatc gaccacggcg
     4081 ctcgcgtcgt tccaccgctc ctcggggatg ccctgctccc ggagccagtc ctcccagctg
     4141 gatgctccct ccggcgggtc ctgaagccgg tagtggagcg cctcggccca gaacgcggcc
     4201 agaccgcttg ggtccgcaca gtcgttcacg atctggatcc tcgtcgccat ggcacgtcct
     4261 cccccgccgg tagggggcca agcctatagc cggggcatcg gcgagaacga tccgggaacc
     4321 gtgtatccac gggtgacgga ggtctgtctt tcgggaacag ggtggtggtc cggacgccca
     4381 tggtcgagct gcatgcacca gcgcaccatg gcggtgagcg gaggtggtcg tcatgcagac
     4441 atgcagggga cgctccgaca tcgttcgttc gggacggtcg atggtcgcgg tgggcgccgt
     4501 ccttgtgctc gtcctcgtgg cctgggcctg cgccgggaag gggaccagcg cgcgacccgc
     4561 ctcctcaccc cccgcgtcga gcccgacctc ggtgatgacg ttgccgagca tccctcccac
     4621 cggcacatct acgccgccga gcaggcgcga gcgcggggtc tggatgctga tcgcatcgtc
     4681 gccccttcca tcgtcgatct ccgcccccac cggggcgtgg gacggcaccg agcttgtcgt
     4741 gagcggacgg gtgtgtacgg atcgggctcc gtaccgccac accgtgacgg cggcgtacga
     4801 ccccgccacc gattcctggc ggatgctgcc cccttaccgc ggcccgaagg gatgcttcga
     4861 gggcgcagat catgcgctgt gggacggaca cgagatgctg ttgctcggcg tgaccaacgc
     4921 cgcgtacgac ccgagcacga acagctggcg aaggctcccc ggctggggtg ggggcggcgg
     4981 tgaggtcaac gcatggaccg gtcggcagac gctgaccttc gggggcggct gctgcgggga
     5041 tcaggagggc atgggcagtt catacgtcgc gcggacgaac cgatggaccc agccacccgc
     5101 cgggccgctg gtcggtcgcc acggcgtggt gggcgtctgg gacggccacg ggttcgtcgt
     5161 cgcgggcggc taccacgagg tcgaggagac gacggagaac gggacgccgg tgaggctcgg
     5221 acccaggatc ctgttggacg ctgctgccta cgacccgtca acgcgcgcgt ggcggtcgct
     5281 cgcaccgatg ccccgcctcc cgggcgttca ccacgtctgg agccgcaccg ccgtctggga
     5341 tgggtcggag atgctcgtcg tcagtgcctt cgggaacctg gcctacgacc cggcgacgga
     5401 ccgttggcgg ttgctccccg acatgaggtt ccagcgtggg ggagcgggcg cggtgtggac
     5461 gggcacgcgg atgctcgtct ggggcggcgc cacgaacgag ggcgagggtg cccccccgta
     5521 cggcgaggcg tacgatccgg cgatcgaccg ctggctatcg atgccgccct cgccgctgcg
     5581 ccaccggtac ccggcggtgg tcgtgtggac cggcacccag atgatcatct ggggcggcga
     5641 tatgttcccc gatggcgcga ccttcacgcc cgtcggctcc tagcgacctc gaaacgatgt
     5701 gggccgtcgc cgatcggttg cgcatggacg gtcgcgggca gcaccgttag gcgctgaagt
     5761 cggcagcgat gggtccgctc cgcatcgagg ctgcaccgcc tggggctacg agtggcagct
     5821 cccggcgccg gagtcgcgga cgtcgccgct cgtgtcgacg aacgaccacg agtacgagga
     5881 cgaactcagg gtgagctcca ccacgccgta ggcgcggatc cccaccacgg cgcccgagtt
     5941 aagcttgccg agggagtacc caccggtgcc gacgatcagg cggagcaagc cgttctccgg
     6001 ggcccaccgc tcgtacacgt ggcgcgaggc cgtgagggcc agatccaccc ccgccgcctc
     6061 ggccttcttc cgcggccact tcaggttggc gcggtggtac accaccatct ggcacaggtg
     6121 gccgtcggcg gcgaggtcgg acttcatcca cgacttctcc gccgaacagt tgatctgtgt
     6181 gcactcggtg ttgagcgcga ccacgtgcca gtccccgagg tcgaagctgt agtagccctt
     6241 ggatggatcg ccggccgccg agccccagta cgtgtagtag ccggcggcgt tcgtcgtccc
     6301 gtaatcgtgg ttgccgggga tcgggtgcgt cttggacttg aaggctcccc agctcgggtc
     6361 gtacgaggac tggaagttcg ccagctcgcc gttctcgtac tgcagatcgc cgagcgcgaa
     6421 cacggtcgtc gggtcgaagg cggtgaccag gtccgccgtc tgttgctggg gcgtggacag
     6481 ggtcggccgg gcgatgtccc ccgcggcggc cacggtgacg gtccccgccg ccgccgggtg
     6541 ggggatctgc agcagcgtcg cgaccagggc gaccaccagc cagagccggc gcatggtgac
     6601 tccttcctcc taagggaggg ccggacggcc cccgccgaac acgatgcgag gcaccatggc
     6661 ccacgttcca cggaaccgca agagccgatc gacgtacgtc ggcccgccga gcgtacgtcg
     6721 gatcgtcgac gctccgcgcg agctcctcag aggggctacg ctcgatggcc catgggtgct
     6781 cggctcgggc tcacgcgctc gcagattctc gcgttccggc gtcgggcggg ggcgctggac
     6841 gagcgactcc atcgcggagc gggctcgctc cggcgtgcgg cgtgggccgg cctgcaggac
     6901 agcatgcccc gagccgcgct tctgtcgatc cacgcccggg tccgcggcac gcacccaacc
     6961 acgtgggagg atccgtcgct ggtgcagctg tggggtcctc gctacagcgc gtacgtcgtg
     7021 gccgcccgcg accgggcgat cttctccctg ggccgactcc cggaagatga ccgcgcccgg
     7081 ggggtggccg aagagctggc cgcgcgcctg gatgccctgc tggacggcgc ccggatgccc
     7141 tacgagcagg ccgggcgcgc gctcggcgtg caccacaacc ggctccggta cgccgcaccg
     7201 accggcaccg tgctgatccg gtgggagggt gcacggcagc ccgtcatctg gaccgtgccg
     7261 ccaccccaga tcgacccgcg cgatgctcgc ctggagctgg ctcgtcgcta cctgcacgtc
     7321 ttcggacccg ccacgccgga gacgttcgcg cgatgggcgg gcatcgcccc gcgggaggcg
     7381 atcgcggcgt tccacgcgct cggccgctcc ctcgcatcgg tgcggacccc gatcggcgag
     7441 ggatgggtcc tcgctcggga cgtgccgacc ttccgggcgg ccccggggcc ggccgctccc
     7501 gcgcgcctcc tgccgagcgg cgatgcctac ttcctgctcc agggtgccga ccgagagctg
     7561 ttggtgcccc accgggagcg acgcggggcg ctctggacgc cccgtgtttg gccgggtgcc
     7621 ctcgtggtcg atggggaggt ggccggcacc tggcgacgcg cccaccagac gctgacgatc
     7681 caggtctggc gccgtctcac gcgcgccgag cgcgacgcgg tcgaggcgga agcggcatcc
     7741 ctccccctgc ccgggatcgg gggagagatc gtcgtccgct gggaggtctg agcagccggc
     7801 tcggccccct gtccccggtg cccaggacca ccaccaccgt cgatcagggg cgtttagctg
     7861 cacatgagtc gggtatcgcc atggcgctcg gccgctcgca gctccgtggg tgagcggaac
     7921 cgggtcgccg aaggagggac gggatggctt cggacgcacg gacggcgcac gacgtcgtgg
     7981 agacggatat ccccgcacgc atggaccggc tgccctggtc cagatggcac tggctcgtgg
     8041 tggtggccct gggcatcacg tgggttctgg acggcctgga ggtcaccatc gtcggcgcga
     8101 tcggcgccat cctcacgaac aagcacaccc tcgcgctgtc cgacgccgag gtcgggctgg
     8161 ccggcgcggt ctacgtggcc ggtaacgtgg tgggcgcgtt cctgttcggg tacctcaccg
     8221 accggttggg tcggaagcgg ctgttcacgt ggacgctcgg cctgtacctc gccgccacgg
     8281 tagccacagc cttctcctgg aacgtgatgt ccttcttcgt cttccggttc atcaccggca
     8341 tggggatcgg cggcgagtac tcggccatca actcggccac cgaggagctg aacccggcca
     8401 gggtccgcgg ctgggtggag ctcgggatca acgggagctg gtgggtgggg accgccgtgg
     8461 gagccgcctc ctccatcctg cttttgaaca agagcctcct gccggtggac atcggctggc
     8521 gggtggcctt cgggctggga gcgttgctgg gcatcggcgt cctgctgatc cggcgactgc
     8581 tgccggagag ccctcgctgg ctgatgaccc acgggcgggt gcaggaggcc gagggcgtgg
     8641 tggccgatat cgaggacgat gtccgccgct acacgggccg cgagcagctc ccagacgtgg
     8701 agggcaccac catcaagatc cggccccgcg agcggaccac gatccgggag gtgctccgct
     8761 cgatgttcgt cgagcgtcgc cgtcgcgtgg tgctcgggct gggcctcatg gtggggcaga
     8821 cgttcctcta caacgcggtc ttcttcacct tcgccatcac gctcgatacc ttcttcaagg
     8881 tgccggcgga gcgggtgggc tggtacatcc tgcccttcgc ggtgggcaac ttcctcggcc
     8941 cgctgacgat cggccggctg ttcgacacgg tgggacgccg cgccatgatc tcgaccacct
     9001 acgtgctctc cgcgatcggc gtggcgatca ccggatacct gttcaccacg ggcacgctcg
     9061 gcgcctgggg cctcaccatg gcgctgtcct tcatcttctt cctggcctcc gccggggcca
     9121 gcgccggcta cctcacggtg agcgagatct tcccgctgga gatccgggcc ctggccatcg
     9181 ccttcttcta cgcgatcggc agcgcgatcg gtggggtggc cggcccctac ctgttcgggg
     9241 cgctggtgga gaccggcaag gaggcgaacc tgttcatcgg ctacgcgctc ggcgcggggc
     9301 tgatgttgct cggtggcgtg atggaggcgt tcatgggggt ggatgccgag cagcggtcct
     9361 tggaggaggt ggcggagccc ctgtcttcgg aaggggtgga gcggggcgac gccgcgtgag
     9421 gatcctcacc aggaggcggg acgacccggt cgatgccgga agggctcgaa ggccatcctg
     9481 gcgagggact cggtggcgta gacgctgacc accgcgccgg gacgggaaga gagctcgcgg
     9541 gagcgctcgg gggtggtcct gagcaggacg accacgtcga gccggtccag caccaggacg
     9601 tcgggcgcgt gcgcgatgtc gtcgatctcc gccaggtcgc tcgccaccac cacgaagcgc
     9661 tgatcctcca tcctcacgat catcggaagg cgggcggtgc ccgggcatcg accctccggg
     9721 tcgttcccgc atggcacccg gtcagagccg tcctcgggcg ggaccgtccg cgctcgttcg
     9781 gatgacctag gctccggcac ctcggaggtc cgtccgatgg cgtcgcatca cccgcgtcgt
     9841 ccgcgctgca gggacaggag cccgaccgcc gcccagccga tgcccaccag gcccagatac
     9901 gtgagtcccc ggtacacgag cacggcggcc acggccgtcg ggccctgcac gccgtaggcc
     9961 acgaatgtcg cgaccatccc cccctccacg atgcccagcc ccccgggcgt gatcccgatc
    10021 gacgacacga ccttcgcgcc tgcgaacgcc aggagcacga cgccccacgg aacatgaccg
    10081 cccgccgcca ggaaggcgaa cgcgagggct gccacgtcca tgagccagtt cgcgagggag
    10141 agcccgaagg ccgtcagcca cgtggcgacg tccgggtggg ggaagctcag ctgacctgcc
    10201 gtccttccga tcctctgggt cgtcctggcc accagcgaac cccgatcgcg gccgaaggtc
    10261 cgctcgaccc accccaagcc ggccgcgagc aggtgcgggt gtcggaacag ggccgcggcg
    10321 ccgaccgagc cgaccgtcac caccgccagc gtggggacca ggaccgtgct gtcgggtccg
    10381 cccgcgagga ccccgccggc cagagccacg gcggccagcg ccgcgaaggc gagcgccccg
    10441 gacgccagct cggcccacgc ggccgcggcc ggttcggccc ccagctgtcg gtaccgcgag
    10501 taggtgtacc cctcggccac cgcgaccccc gccgggatgg acatcgcgat cgcgttgctg
    10561 gccagggtca ccaggttcat ccgccaccga tccgcgtgca cgccaccggc ccgcaggacg
    10621 atcagctcgg cctcggccag gggaggcacg gagcccatct cgacgatgaa cgccagaccg
    10681 acccagtacc aggtcagcct ggtcacgatc gacgcgaacc cttcgaacag atgtcgctgg
    10741 ctgaaggcgg ccacgcccac gcccaccagc gccccgatct gcagggcgat gcgcgcccac
    10801 agcttccggc gccccgcgga tccctcctag agcttccagt cggtgagcga cccgatcggg
    10861 agcagcccgt aggagtcgac gatgtccaac ggggtgtcct cgttcatgac cagcgccacc
    10921 ggcctcgcgt attcggcacg gaacccgccg gagtgctcga tcaccttccc ggtgagccgc
    10981 accacgcccc agaggctcgt cttgccgtag tagtcggtcc gcacgcggtc gggccggtcg
    11041 tacgtgggct ggctcttgga ggcgtagatc ccgcaggagc acccctcggc gggggcggtg
    11101 tgcgatgaga acaggaacag ggtcttcggg cagtacgcgc gcatgggtcg atccgtcggc
    11161 caccggccgc gtccccaggg agataccgag tacagcccac ctcgtcgtac gcgccagacc
    11221 cggtacccgt acaggggttc acgccggaac tgcatccggt tccgtcaccg gctctcgcac
    11281 cggggtctgg gcgggttcct catcgggaac cacgatccag gtctgttcct cctcgccgat
    11341 ctgcatggtc gttcccctcg aggaggtggg ttcgctctgg gtcgaagggg cgatcccacc
    11401 gagtcttccc tcgatgccgg atggggggtc cccgccgacc cgacacggga cccaggtgaa
    11461 ggcttcccga gatccccgcc ctcaaacctc tgggggccag tcctcccgct caggactgaa
    11521 ccggggcccc gatccgaccg ataccgttcc tagccgaggc aaccgtcgcg acgccatcgg
    11581 ccggtcttca cgcgaggagg aggagcccgt catggatccg cagggtccgg tcggcggccc
    11641 gcggccggcc cccccgccgc ctccttcagc gctgcgggcg gagccgccca ggaccgcgtc
    11701 cccgcgctcg gcgcgccgag ggatccgggt gctcgcggtg gtgctggcga gcctgttggt
    11761 cgtcgccggc accggcgcgg cggccgcctt ctacctggcg cgcggggcga gcgaggagct
    11821 catccagatg atccccgcgt ccagcgacgt ggtagtcacg gcgtacctcg acccctcggc
    11881 gggtcagaag ctcaacctgc tgtcgctgat gcaccggttc ccggccctcg ccgacgaccg
    11941 gcagctccgc gggcggatcg acgacctgct cgaccaggcg ctcgaggatg cgggtctcag
    12001 ccatcaggac gtgcggtcct ggctcggatc cgaggtcgcc gtcgccatcg agctgcaacc
    12061 gggcctgggg gatccgacca cggcgatcct gatcaccagc acggacgacg acgcggcaga
    12121 gacggcgatc gagcaggggc tgcggacctc gctcggggcc ggaggtagcc gcggataccg
    12181 aggggtgagc ctgcacacct acggcgccgg ctcatcggcc gtgaccttcg ggatcgtgga
    12241 tcacgtggtg gtgctgggga gccgtgccga cggagtgacc cgtgtgatcg acgtggccaa
    12301 gggcaccgga cgcgcgatcg gcgacgaccc ccggttctcg aggaccatct ccacgctgcc
    12361 ccgggacagg ctcggcttcg cgtacgtgaa cgcggggggg atcgttcggg cgtcgctcga
    12421 ggcgaacggc ctgggggcgg cgatcggggc ggggaccggc cttgacaccc tgcgtgcgct
    12481 cgaggggatc ggcgtgtcgc tgtcggccca tcccgacggg ctggccctcg actacacgat
    12541 cggtgtggat ccctccaagc tgcagccgga ggcccgcgcc cagctggagc aaccgtcggg
    12601 ccagaacggt gtggtccggc tggtccccgg cgacgccttg gcggtcatga cccagcgggg
    12661 cctggctgcc ggtctcaggg ccgccgtcga ccaggccctg gccacccccg aaggcgaacg
    12721 ggtgcgtcaa cggctgggcg tggaccagat gctggccgcc ctcaccggag acgtggcggt
    12781 ggaggtggcc cccgaagccc ccgccacgcc gatcggggga gccgtggtgc tcggcgtgag
    12841 cgacgccaag gcggtccaac gcacgctgga tgggctcgcc cgactggcga tccgggcgca
    12901 gcggcgcgcg gagggcgccg tccccacccc cttcggcgac ctcacgggaa ccgtggtgct
    12961 cgcgaagagc ttcactccgg aggctccgac ggtgcggctg cggaccacca cctatcgggg
    13021 cgtgaggatc cggtacgtgg aggacccgtc cctttccgcc atgagcgggg tcctgccggc
    13081 ctatgcggtg gtggacgggg cggccatcat cgggtcgacg cccatggacg tccgcaaggt
    13141 gatcgacgtg agggacggga tccgggcgag catcgtggac tcgccgagct acttggacgc
    13201 gctggcccac gtgcccgggg acggcaccct gtacgtggac gtggcggggg tcgtctcggg
    13261 gctgggcccg cagctcccgc ccgacgtccg gtggaacctg gagccgctcc gagcggtggt
    13321 gctcggaagc acgtcgtctc cctcccggat caccggtcgg ctgttcgtcc ggatcgggtg
    13381 agcgggctcg aggggaacca cgatcaggcc gagcctcggc ccggctgccc ggaccagcga
    13441 tgcggcggta ggcttcgcag cgtgagaggc gatcgtatcg aggtcgtggt ggactcgggc
    13501 aacggggtcc tgaggtacga gatcgaggcg accagggccg gtcggcgggt ggaggtctcg
    13561 acctcgagga gcgtgatcga ggtgaccgag gtcaccagga ccggcgcgcc ggtgcggagc
    13621 ggccggttca tggccgatcg ggtggtggcc ctggtcgagc atcccgcccc cccgccgccc
    13681 acccggggcc gccggccccc tcgaggacag gtctcgctgg tctgatggac gacgaagcgc
    13741 tcctcgcgct ggccgagaag accctgaccg agatcaagat cgcccagggc ctgtccgacg
    13801 agcacgcctc ggtgctggcg gcgctccgga tccggctgaa gggcagcgcc ggcggctcgc
    13861 tcgaggagct gctccaggcc gcgggcgacc tcggtggcga gagcctggag gacaagctcg
    13921 ccgaggccga gcgccggccc aagccctccc tggacgacct gctgaagcag gacccgaagc
    13981 ccaagcgcga ctggccttcc tgaccggcgt gcccccctcg gtgaaccgcg gacggcgccg
    14041 catcgaacat ctgccgatgg accttcgctc gccccgaccg gtgatcgcgg cggcgagcct
    14101 cggcctctcg ctcctgctca cggcctgcgc ggcggtctcg tcggggcccg ccccggccgc
    14161 atcggggagc ggcgctgcat ccgccgctcc gagcgcctcg ctctccggca cctcggtgct
    14221 ctccgcggcc gatgcccagt tggcggacca ggtgtgcacc cagaacagcc ccgaggtgct
    14281 gctccgcacg taccgaggga cgcagctcgg gcgctcgggg gacattcagc tgatcccccg
    14341 ctacccggac ttcatgggcg ccggcctacc ccacgccacg ccgtacgcct acacgcagga
    14401 cgtccccctg ttcctgtacg ggccggggta cgtgaagccg gggacctaca cccggccggc
    14461 ctcgctgacc gacatcgccc ccaccgaggg ggccatcctc cagttcccgt tccacgcccc
    14521 cgacggggtc gcccagaccg aggccctgct cccgagctcg ggccggcccc cgccgaggct
    14581 cgtggtcacc ctggtctggg actcgggggg catggatgtg cttgacgcct ggaagcgcga
    14641 ctggtcgtac cttcgatccc tgctcccgca cggggcgtgg ttcacccacg ccacggtggg
    14701 cgcctcgcca tcgaacaccc ccaccggcca tgccgagatc ggcaccggcg ccttccccgt
    14761 ccacaacggc ttcgtggacg actacatcga gctggacggc aggatccaga agcccaacgc
    14821 gaacgggccg gccttcctgg aggtgcccac cctggccgat ctctacgacc gagccatggg
    14881 caaccggccg gtggtgggcg ccgtggtgac cctctcggcg cacgtgatga tgatgagcca
    14941 cggctcgctc tggggcgggg gcgatcggga catcgcgatc acgcgcgagg tcacgaacgc
    15001 ctccaccgcc ggcgccgagg ccggccggtg gaacctcagc tcgcagatgg ctccgtacta
    15061 ccggatccct gcctacgtga actcgctgcc cccgctggcc cactacctgc gaccgatcga
    15121 cgccctcgac gggcagctgg acggcgcgtg gcggggcaac tcgatcgccc agctgcagga
    15181 cgggttcgac acgcccgcca ggaccccgta ccagacgaag ctgatcgagg ccgtgatcca
    15241 gcgcgaaggg ttcgggaagg acgacgtccc cgacctgctg tcgctgaact acaaggcgat
    15301 cgacgtggtg ggccacctgt tcagcgcgaa ctcggtggag atgtccgaca cgctccggat
    15361 ccaggaccag gccctccgtc agctggtggg gttcctgaac gccacggtgg gcaagggtcg
    15421 gtgggtcatg gtgctcacgg ccgatcacgg cacccagcgt ctgcccaccg tttccggcgc
    15481 gttcgtggcg aacatcgacc ggttcaccgc cgacatcgag caccgcttcg atcacgacgg
    15541 cgacggcacg ccgctgctgc tcagggtccg acccacccag ctgtgggtcg acccggccga
    15601 gatggcccgc aaccacgtca ccctggacca gatcagccag tacgtgatgc agctcaccca
    15661 gtcccagctc acgcgcagcg gcgtccaggc cgatccggcg accgcgaacg accgggtgtt
    15721 ctcgggggcg ctgccgtccc ggctgctgcg atccttgccc tgcctgccgc cgaacgtcgg
    15781 ctgagccgcc agtccgggca gggcccggtt cgatgcgggg accggtcagc ggtccgcggg
    15841 tgccctcggc ccccgtcggc cgcggcggga gggcctatcc tgcatccgtg gacttcaacc
    15901 aggcgccgat cctcctgttc tgggagacca cgcgggcctg ccggctcgcc tgccgtcact
    15961 gccgggccga ggccatcgcc accccgctgc ccggacagct gtccacgtcg gaggggatcc
    16021 ggctgatcga ggacctgcgg gggttccggc cgcaccgccc ggtgctgatc ttcaccgggg
    16081 gcgatccgtt catgcgggag gacctgtggg agctggcgga ccgagcccgg ggcaacgatc
    16141 tgccgatcgg gttcgccccc agcgtcacgc cgctgctcac cgagcgggcc gcccgccgca
    16201 tgcgcgaggc tggggcccgg accgtctcga tcagcctgga cggggccgac gccgcctcgc
    16261 acgagggcgt ccggggtgtg ggggaccact tcgcccggac cgaggaggcc gtccggatgc
    16321 tggtgcggga gggcttgagc gtccagatca acaccacggt gatgcggcgc aacgtggagc
    16381 agctcgcgga catcgccggg ctggtggccg cctggggcgc cgatatctgg gaggtgttct
    16441 tcctgatccg ggtggggcgg ggctcggagc tcgaggagct cacgccgctc gagaacgagg
    16501 acgtggtcca gttcctatac gaggcctccc ggtacggctt catcgtgcgg accgtggagg
    16561 ctccgttctt ccgccgggtg gtggcctcgc gccgagagct cgatccatcc gccgatcccg
    16621 ccgccgcctt cgggctgggc ccgatgtacc ggtacctggc cgaacggctg cggtcgaccc
    16681 tcgggacgcc gggcccttcc cgggcgcagg gcgtgggcac ccgggacggc aaggggatct
    16741 tgttcgtggc ccacgacggc gacgtctacc cggccgggtt cctcccggtg cgcctgggca
    16801 acgtgcgcca ggacgacgtg gtggcgctgt atcgccacca cccgatgctc cgagcgatcc
    16861 gcggcgctgc cttctccggc cggtgcggcc ggtgccggta cgccgatctc tgcggcgggt
    16921 cccggtcccg agccttcgcc gccaccggcg atcccctggg cgaggatccc gcgtgcgcct
    16981 acgagcccgg accggccctg gtcacctaag cggcgggtca gggcaccgtc gagacctgaa
    17041 ggcggtcgcc gccggtgacg atcactcgcc ggcccggctc gagcggggac ccgtccagcc
    17101 tgccgcccgg tccggagggt gtcacccgca gcgaggccag gccgctggcc acccgcacgt
    17161 ggatcagacc gggctccggg tagggctggg gccgcaggcc tccgtagtag taggccagga
    17221 tctgagaagc cgagaagccc cggcgggcct tcccgtacgc cccccactgg accatgccca
    17281 caccgtggcc ccaccccctc ccggtgaaga ccgcggcccc gcgcgtggag gagacccgca
    17341 cccaatgcga cgggatggtc accggcaagg agtcggtcgg gtagtgcgcg ggctccaggc
    17401 acggcgccca cgcgtcgacg atgccccgga agctgtcgag gtccagtgac cgcgagctct
    17461 ggcccgccga gagcacgatg gtcgagccct gcgactggat ccgtgccacc cgtcgatggg
    17521 cggtccaggt gccgccctcc cgcaggaacc tggtgagatc cgagaagggg atgcggaccc
    17581 gccaatggga ggtgggcgag gcgccgtcgt cccgctccac gaccggccgg aggtagggga
    17641 gcggggcgct accgaagacc tgctcgttgc cgtacgtgtg cccgttcgac gtggagaagt
    17701 acacggtctc tgcaggccgc ccctggtaca gcaggaccag cccggaggtc gaccggaccg
    17761 cccggtacca gcggcggacg tcgggctggg gcgggaccgg gatgcctcgg tagacctggc
    17821 aggaggtggt ggcgcagatc ggcgtccgga gggtctggcc ctgggacccg ctccatccgg
    17881 tggtggccag cgcgtagctc cgggcggcga tggcttgggc ctggagtgcg gccgtgggcc
    17941 aggacggcgg gacctccgcg atcccctcga ggtagcgctc gaacggcagc gtgagggtga
    18001 gcgagagcgt gccgtcctcg gaacggacga tccggaggtt gcccgggtac cgcgcccgga
    18061 acgatcggga cggcggacgg acgcatcggg aggccgcgtg cgggtagcgg ccccgaagga
    18121 gcagggagcc atggcccggc gcgccgatgc gcacggcgcc ggccgtcgag gccggtgccg
    18181 acggcgtgcc ggtcggcgga ggttccgaga tgggagaggg tcccgggctg cccgaggccg
    18241 aggccggcga cggcgccggg ctccccgagg tcccggcggg cggcggggag gaccgggacg
    18301 agcctccggt gggcgccgag ctacacgccg ccagagcgac gaggaggatc ggcaggatgc
    18361 agccggcgat ccgccgggag agcgggcgcg ttcccacgtc gctcgcacgc tatcagccgg
    18421 cgcggttcgg tgggccgacc ggggggccag gtcgggacgg gggccgcgcg gctcggtagc
    18481 ggctgggccg cctggagcct ggcgcggccg gctgagctcg ggaagaccgc gcttcgggtc
    18541 gggagacacg ctcgagctcg gcgaccggcc ccccgggacc gggaccactt cccctaaggc
    18601 cggacctcga ggcccggaag gctggtcgcg ggcccggctc catcgggacc gggtccggga
    18661 gggacctcga cgggctggag gtgaggagga tggcgaccga ggcctcgtat cccctccggg
    18721 tggaggcgga gctggagcca cgcctttccc ggtggctgtg gctggtgaag tggttcctgg
    18781 cgatcccgca tttcatcgtg ctgggcttcc tgtggatcgc cttcagcgtg ctcaccctgg
    18841 tggcgttctt cgccatcctg ttcacgggcc ggtaccctcg ctcgatcttc gagttcaacg
    18901 tgggggtcat gcggtggacc tggcgggtga gcgactacgc gtacggcgtg ctgggcacgg
    18961 acgcttaccc gcccttcacc ctcgaacccg tgccgggcta tccggcccgg ctcgaggtgg
    19021 cctaccccga gcatctgtcc cggggtctgg tgctcgtgaa gtggttcctg gccatccccc
    19081 acctgatcat cgtggggatc ctggccagcg ggggcacctg gctcgcctgg aacgccgacc
    19141 atctcaactg gggggccggg ggcggcctga tcggcctgct ggtgctggtg gcggccgtga
    19201 tcctggccgt gacgggcacc tacccacggc agctgttcga cctgatcctc ggtctgaacc
    19261 ggtggacgct cagggtggcc gcctacgtct cgctgatgac cgaccggtac ccgcccttcc
    19321 ggctggacat gggcgggaac gagcccggcg gcgtcgggac gctgacgatc cccgcccccc
    19381 cgagctccga gcccggaccg acgcttcccg caccccctcc gtcggggggt gcccggcgcg
    19441 gcggctggac cggcggccgg atcacggcgg tcgcgatcgg ctcgctcctg gccctggtct
    19501 cgttcgccct cttcgccggc ggcgggttcc tgctgtgggc cttcggcacg caacgggacg
    19561 cccagggctt ctacagcacg cctgagcgga cctaccaagc gaacacgtac gcgttgatca
    19621 cccgagctcg ggtcaccgag cccggaccgg gctggttctc catccgcgga gggggctggc
    19681 tgttcggggc ggtgtccgag gaggccatcc ggatccgcgt gaacgagcaa gggggaggca
    19741 acgtcttcgt cggtgtggcc ccgtcgacgg acgtggatcg gtacctgtcg ggcgtggcga
    19801 ggaccgtggt caccgatctg ccggaccccg actaccggag cctctccggg agcgctcccg
    19861 ccggagcgcc ctcgtcccag gccttctggg aggcctcggc cacgggcgcc ggcacgcggt
    19921 cgctgcgctg ggatctccat gaggggtcct ggacgatcgt ggtgatgaac gccgatggct
    19981 cccgggggat cgacatccgg gccgatgccg gcgcgagggt ccccgctctg ccctggatcg
    20041 ccgcggggct gctgatcggt ggcgccgtgg tgctggcggg agccgcgctg ctgatcgcga
    20101 tcccggtctc ccgcgcgaac cggagcacca gacgaggggg ctcgatgccc tgagcccgtg
    20161 agctctcggg ccggcggtgc tcaggcggcg tcgtcccgca ccaccgctcc gatcctcggc
    20221 gaggccaggg ccggcgactc gccctcgagc caggactcga ggtcgggcgc ccggctgggg
    20281 ggacggccga ccaggtacag gccgacgatc acgcccagcc cgccgatcgt cacacccagc
    20341 gtgacccaca gggcggtggc gacgcccacc catcggctcc cggccgttcc gatggtcacg
    20401 gcgatcgcca ggatcaccgg ggccaggacg aagtcggcca ccgagcgcac cagctcgacc
    20461 agggcgaagg tgcgccccac catcatcgcc ggcagggcga acgcggccat ccacagggcg
    20521 ggggagaccg tggcgccggc tccggtcccg agcagggctg ccgagatcag gatcacgatg
    20581 tccccgccgc cccggcccgc gaacagcacg gccccgccgg cggcgatgca ggtcatcccg
    20641 gccaggacca ggtgcggcag gaaccgggtg cggagcagca tggcgagggc caccgaagcc
    20701 acgatcgtgc cgagcaccat gggccagtag agcaggccca cgtgcgtggg ggactcgtgc
    20761 accgtggtga gcaggtactg ctggccgagc tcgaggaacg agacgaacgc ccccccggcg
    20821 accgcggcca cgatcgttcc ggcgaccggg atcgtgttcg acatggtgcg caccggcgag
    20881 agcggctcgc gcttgtggta ctgggagaag agcaggacca ccagcgccgt cagcccgacc
    20941 gtgaggggca cgatgaacag cggcgaggag aagggggtgg gatcgaccca ggaggtggcc
    21001 aggaaaggca ggaccgtggc gaccagggcc aggctgaggg ccgagcggtc catggggaag
    21061 ccggggttca tgggctccgt gtgctggagc acgacgaggc ccagcgcgag gttggccgcg
    21121 cccaccaggg cgacccctcc caggaaccag cgccagtgtc cggcggccgc agccggaccg
    21181 cccagcagcg ggccggccgt caccgccccg aagaacccga tgtcgatgaa cgccgccgtg
    21241 gtcctcatcc ggcgcgccgg gtaccggttg accagggggg gcacggcgat caccagcagc
    21301 aggccggtgg cgaacccctg gagcacgtgt cccgccccga acgcgatggg ctcggtggcc
    21361 accgcggcgg tgagcgatcc caccacgaag ccgctctcgc agatcacgaa cagggtgcgc
    21421 tgggagaacc tctgcacgat gtcgccgccc agcagcgccc caaacgcgta gccggcgatg
    21481 gccagcccgt cgaagacctg gatcgtggtc cggctggtgc ccaggtggtg ggcgatgacc
    21541 ggggacagga acacgaaccc ggtggtgacc accaggaacg gcgacagcgc caggaccacg
    21601 ttcgtggtga cggcgccata cccgggaggt ggtgcgtccg gcatgcgtcc ttcgttcccg
    21661 gccctcccgg ttccgaaccg gataccgagg gagcgacctt cgtcctagtc cgatggtcgg
    21721 tggggccccc ggggcgggtg agtcccttgt tgcccgttcg ggctatcgac tgtcactcgc
    21781 tgctgtcgca tagtcgggct acagaacatg aggacgttca ccgaccagca acgctacgtg
    21841 gtggtgctcg gggacgcggc cgacgaggtc tccgaggcgc ccaccgtcac cgtcctcgat
    21901 cgcctggatc gggtggtgct cctcgaggcc agctaccgga cggcggtgga gctgcggcac
    21961 cgggacggcg tcatcatcca catctacgaa cgcgagcggg acgcgcggcg cgcgttcggc
    22021 ctgttcgtcc actgaggggc cgaggcgccc cagcgggcgc cggctcgact cggggtcagc
    22081 ccgcgtcgcg gacgccgacc tcgtcgaggt gcctgagcag gtcggccggg tcctgataca
    22141 cgcggtacgc gccggcccgc tccagttcct ccatcccgta gcccccggag agcagcccga
    22201 tcccgaggga tcgcgcccgg cgggcggcga gcaggtccca cacgctgtca cccaccacca
    22261 cggcgttcga gatgtcgacg cccaaccgca gcgccgcctc caggaacagg tccgggtcgg
    22321 gcttggcccg ctccaccagg tcgcgcgtga tgaccggcac gccctccggg acctcgagcg
    22381 cgtccagggt ggggccggcg ctctccaggt gtccgctggt ggccacggcc caggccacgt
    22441 cgagctcggt cagcttccgc agcagctcgc gggccccggg caggggctgg acccggtccg
    22501 ccaggcgggc atacgcctgg gcgtggcctc gctgcagccg gtcctggatc tgcggcgaca
    22561 gtggcacccc ggtctcgcgg agcagggctt gggcgaacag gccgccgctc atgccgatcc
    22621 tccggtggat ccgccagacg gagagctgga tgccctcggc ttcgagggcc tcacgccacg
    22681 ccaacacgtg ctggtacacg ctgtcgacca gcgtgccgtc caggtcgaac agcatggccg
    22741 ggggaccggt catccatacc tcccatacag gtcgctgtgc ggatcgaaga agccgccggc
    22801 cggccgcgcg tgggcgtgcc agtggtcggg gatccttcgg cgctccgggt cgatccagta
    22861 cccctcctcc ccgtagcgct gggccgccac acggtccagg tgtgccagga gccgggcctc
    22921 ctcctcgggg tcggggaggc cgtgcgtgcg ccagaccacc atcggggtgg cgcacaccag
    22981 gcagtccgcc acccagcagt cctcgtcctc gaagtgccag gccgtgacgt gctcggcccg
    23041 gcacagcagg cagtcatgct caggcatcgg acctcaccag ctcgaccacg tcgcccaccg
    23101 cgaccccgtg gtgggcgaag aagccccggt tggcctccac cgctcccacg aacggcgcgc
    23161 cggcatcgta cgtgcggcag ggctcccgcc ggcagggcac catctcccgt agggccacga
    23221 tcctgccgtc gtggtcccag aaggcgatcg acagcgggat caacgtgtcc ttcatccaga
    23281 accgagcccg cacgggctgg tcccatacga acgccatccc ctggtcggcc ggcagcgagg
    23341 tccggcccat gagcccccgc tcgcgctcgg ccggcgtgtc ggcgatctcc acccggaggg
    23401 tcacggtgcc gccggcggtc cggatccgaa cgcgatcggc gggcgtggtg gcggcagcgg
    23461 gccggcctcc tcccgagcac gcggtgacca gggaggcgag caccacgagc agcgcgagca
    23521 cagggcgcgt cgcgcgatgg ttccacgtcg ggcgagcagg tgccattgct atcatccctt
    23581 ggccgccgga gtagctcagt cggcagagca cctccatggt aaggaggggg tccggggttc
    23641 gagtccccgc tccggctcgt cgcctcgaca ggtcctcatc cgcggccctg ggcagggcat
    23701 ggcgaggtcg cgcttcgtcg ggaagccgaa cgcgttgaca aacgcgcgac cggcctgctg
    23761 gtcccgccta ccgagatggc gcagaagagc cggcaaccgc ttaacgtttc gaaccccatc
    23821 gcccccgtag tgatggaggg ctagggtctg ccgcgtgctc gatgaggggt cccgacgacg
    23881 cctggaaggc gcgtaccgcg agtacggcgc ggaggtctgg agggcgatct atgcctattc
    23941 gggaggaagc cggcatatcg ctgacgaggc ggtggccgag gcgttcgccc aggctggccg
    24001 cggcctggac cgaatcaggg atctccggcc ctggctgttc cgggcggcct tcaagatcgc
    24061 ggccggcgag ctcaagcagc gacccgcgcc gagcctacgt cctcctgccc ctcaggacgg
    24121 acaggggatg atggagctgc tcgatctcgc ccgttcactc acgagtgctc agcgccgggc
    24181 gttcgccctg cgcgacgtgc tcgggttctc gagccgagag gccgccaagc tcatggggac
    24241 gagcgaggtc gcagtccggg tccacctcca cgccgcccac cgtcgcctca gggcgctagt
    24301 gctggaggcc gagtcgtgag gatcgaggat ctgcgaaggc tccgctccga agagccactc
    24361 gacctgtggc cagagatcga gcgccgcctg tcgcttcctt ctcccggtgg ccgaagccct
    24421 ctccgacgcg tctccaccct ggtggcggcg gtggggatcg ccttcatctt cggcctgtcg
    24481 ctgtacgcgc tgaccgggtt gatcggaggt cgcggcgcct cggtgggaag cggcggcatc
    24541 gtcagggtcc cggtccccgc gccgcaaccc ttggcggcgg gagagggcgc ggtctgggtg
    24601 gtgggcggcc tctcagacca gggcgggctc tggcgggtcg accccacgac cgatcgggcg
    24661 cggctcgtgg cgggcaccga gggggcgtgg tggccggcca ccggcgaagg agcggcctgg
    24721 gtcacgacct gccgccccgg ggacaagacc gactgtgcac gggccgatct gcttcggatc
    24781 gaccccgcct ccggcaccct gaccgccagc ctgcggctgc ccgccccgcc catgcagatc
    24841 gccgcgggcc tgggctcggt gtggatctcg gcggaccagc acctgctcaa gatcgacccg
    24901 gtgagcatgt cgatcgtggg cgagatcccc ctccacacca acctgctcgg caccgcgggc
    24961 ggcttcctat gggccactgt gggaggagcg ggcgcaaccg gcgtggacaa gatcgaccct
    25021 tccaccgggc gagtgctggc ccacatcccc ttcggggatc cctgcacctt cgcggccagc
    25081 ggggatctcg tggcggtggc aagctgccag ggcgggctgc cccccgggag cgggccggat
    25141 cacttggtcg ggatcgatcc gtccacggcc acggtcctct acgacgtgcc gctgtcctcc
    25201 tacggggggc tagccttcgc cgctggatac ccctggatcg cctcatggac gaaggacccg
    25261 gagcgggtcg aggtccgcca gctcgatgcg tccaccgggc tgcccaccgg cgtgagcgtc
    25321 tcgctcggac cgggaccgcg tccctggatg gagctcggca tgggcgcgcc gggcgtatcc
    25381 gtcgccacgt ccgacggctc cttctggctc acccacgtcg atgccgatga cgtcgttcgc
    25441 gttgatgtcc ccgtcgctga cgccagtcgg agctcctcat cggccggcgc cgcgcaggga
    25501 cggtgcgtca aggtgggcgc ccaaggtgat ttcgatgacg atggcactcc cgacacggtc
    25561 aggctctacg acgaggtcgc ccagggccag acctgccatc aagacatgtc ccacttgctc
    25621 cacctctccg ctcagttcgg gtcgggagga ggcttcgaca tcccgttctc ctactgccag
    25681 ggcggcgcgt gctcgcgggt cctgcaggcg gtagacctgg acggcgatgg tcgctcggag
    25741 ctggtgatcg aggtgggccc cggcgccgcg atcgattccg tggagctctt ccgggtatcg
    25801 gcggacggga tcatcccgct ccgggtcgct ccccccggcg accccggctt cgtcgagccc
    25861 ggtccggcga tcctcggggg ctcgttcgac tcgggcggca tgagccccgt gtcgtgccgg
    25921 agccagccgg atgggaccac gcagatcgtc tcggtccacg ccgcagcgac cggcgatctc
    25981 aacacggctc cttgggacgt gcaccgaacg gtgctcgcgc tgcagggcga cacgttcggg
    26041 gtcgtgggca gctccgatca gacggtggaa cacttccagc cgagcgcgcc ggtgttcagc
    26101 tgcgccccag ggtcgcccta gcgcgtccga tccaccggcc catcgagttc ctgcagaggg
    26161 acctcggcac gacccgggct ccgggaagaa gatggggatc ccggcgcgag actaacgccg
    26221 gcgtggtccc gggggttcat gtacaggatg ctggagcagc aggcgggccg gcggaccgtg
    26281 gtggagttcg actccttcta ccgcgagcac tacgcgcggc tcgtccgcgc cctgctggtg
    26341 ctgacgagcg acccggccga ggccgaggac ctggcccagg aggcgctgtc gcgcctgtac
    26401 gagcggtggg accgggtcca ggagatggac tcgccccagg gctacctgta ccgcacggcc
    26461 ctgaacctga accgcaaacg ggtccgccgg ctcctggtgc gcacccgccg agcgatcacc
    26521 cttcgggcgt cggcgccgga ggatctgccg gccgccgagg atcggatcgc tctgcaggcg
    26581 gcggtggcca ggttgcctcg ggcccagcgc gaggcggcgg tgctggtgga gttgctcggg
    26641 ctggacggga acgaggcagc cagggtcctc cggatcagcc cgggggcggt ccgctcgcgg
    26701 ctcctgcagg cccgccgcaa cctgcgcgca caccttggag gacccgatgc ctgaggacct
    26761 gcgagatctc ctgcgacgca ccgtggagtg gtacgagccg cggccgcccg acccgacgga
    26821 tgccccgcgc cgcacccgag cccgggctcg gcggggcaag gtcgcggtgg tcctgctcac
    26881 cttcgtcctg gcggcggcgt ccttcgggtt ggtgttcgcc acgttcgccg gccggaacaa
    26941 cccggcggac cgccctcccg cgccggctcc ttctcccgcg tacaccaggc agatccaagg
    27001 gcagctgagc gtcctgcagg ccaagctggc cgacgtccac gggcagctcg cgtcggccgc
    27061 cgggctggtg ggcaggctcg cggaccggta cggggaggtc gttagggcgc tgcaggccgc
    27121 acccaacccc acccaggcga tgcgcctgcg ggccgatcgt ctgcaggccc agctggccac
    27181 cgcgaaggcg cggacgaagg agttgaccgc ccaggagcag gccctcgtga agcagatcaa
    27241 ggccctcgtg gctcggctcc aagagggccc gagccctcgg agctctgctc agcccgaggc
    27301 ggcgatcgac gtcgggctcg cccggcggat ctgcaacgtg cagcggatga gcggcgacgt
    27361 gaacggcgat gggtcggtgg acacggtctg gaccggctcg ctcgtggggg ccgaggggag
    27421 ctgtccaacc gagccgagcc ggcatcgcgt gctggtcatc gatctggatc gtgatggccg
    27481 cgccgacgtc cagagtacgg ccatggactg ccgcacctgg tgcgtggtct acgccctacc
    27541 cgatctgaac ggcgacggca cggccgagat cctggtcaac gaagggcatc tcgcctatcc
    27601 ggcatccgcc ctcatcgggg tgtatgagct cgtgggccgt gatctggtgc cgatccgctt
    27661 ccgggacgga tcgaaccgct tcacgctctc ggactcctgg cagggcttcg cgggagcgta
    27721 ctgctcctcc tccacgttca cgacctggga cggatggacg gatgggagag gtgccctgcg
    27781 gcgggtcgcc taccggacct acgcggtgaa catctcgtct cttggcgcct ggaggttcga
    27841 gctggtctca tcgcggacgg agcggacgaa ggagcgcccg tctcgaacag gatgggccag
    27901 cctgtgcggc tctcccacga cctccttcgg ctaaggagat cgctggacga tcgcacgcct
    27961 gccagggcgg ggtgcctggg ccctcactca ccgggtaccg gatcgcggcg gaagtgagca
    28021 gcgggccggc ggcgagatca cccggttgtc ggtctcgtcg agcgatcggt ccggaacctc
    28081 ggagaaccgt cctcccctct tcctcggggt ctggactggg tcgagctcca cgtggcgcga
    28141 gcggctcagg acccgtggat cgagccgtcg gagagcgagc cgacgtccgc cagctcgtac
    28201 acgtcttcgt tcaagttccg acagacgaca cgagagaaca gcaggtcgga gccgcccgtc
    28261 gaggggtccg gggcgagcga catcgtggca tcgatatcga tcccgtccgg catggccagg
    28321 acgcgcgtcg gggtctgcga cgggtcaagg acgggtaccc gtaggatcac gaccttgcgt
    28381 ccgcagctca tcgtcgctga gcgcacgtag tagacgctcg cgttcgtctc atcgacgacc
    28441 ggggcgtact ggggacggtc cttcctggtt gggatctcgc tcgtcgtcgc ggtcgcgatg
    28501 tcgtacacgt acgcgctgca cgaggatgag cacagggtcc aggtggcata cgtctcgccg
    28561 acggcgccgg tcggtgggtt gaacttcccg gtgcgccagc tcgtgagcct cgtcctggtc
    28621 ccggcgctgc ggtcgtagag ctcgatcgcg gtgcgccaca cctggttctt ccggtaatcg
    28681 aggttgtaca ggatgtagct gctcgagatc cgcggtcccc attcccaccg cctcgtgttc
    28741 acctcggtga tcgaggtccg ggtctgtgtg tccaggtcgt agaagtacag atccgagggg
    28801 ttggtacgcg ccgacagatc gatctgctgg tagatgaccg tgtcggtacc cgggtcgaac
    28861 cccccggtga agccctgggt gttctgggca ttcagccgga tcctcgatcc gccggcgagc
    28921 gggcgggcga acgcgttgga gagggt
//
LOCUS       DASQQQ010000300         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_374523, whole genome shotgun sequence.
ACCESSION   DASQQQ010000300 DASQQQ010000000
VERSION     DASQQQ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374523"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /gene="tatC"
                     /locus_tag="VE646_11440"
     CDS             <1..539
                     /gene="tatC"
                     /locus_tag="VE646_11440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZD80637.1"
                     /translation="FIGAVDAVVIKLKVVVFLGLLIALPVVLYELWSFIVPGLTKHER
                     RMAIPFVLSSVILFGMGAALAYFTLPRALGFLLGFAGPGFTPLLTGDKFLGFVMLVAL
                     AFGLSFEFPIVLVFLAMVGVISSRQMKGWRRGAIVFIAVFAAVITPSQDPYTMLAMML
                     PMVLFYEGAIIVSRLLGH"
     gene            550..1302
                     /locus_tag="VE646_11445"
     CDS             550..1302
                     /locus_tag="VE646_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80638.1"
                     /translation="MAIKGKSKTRSRRAVTPGPRPVYVPVKKPLYARREIQVAVVVLL
                     VIAAAGGIWYGVAHSRTQARAREQQRLMRTVASAYQVEVNQALSGVGQPTPPVGFSAM
                     PGLKPDIQGLRKGSVDPSKATADAGSFAQTASKAADAFDKIDATSLVSGKGITDQVFV
                     LNLLNSRTRMEEALKLYGNAARLLGDAATATGGTRTELLQRASSVTDVASGLFAEGYN
                     DYLNAQDAAGILQPTLPSSGSPVGGSPLGGGS"
     gene            1305..2042
                     /locus_tag="VE646_11450"
     CDS             1305..2042
                     /locus_tag="VE646_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZD80639.1"
                     /translation="MSDGLLLLHAFPLDARMWEPQVSKLGSELPVVAPDLPGFGGSPP
                     AADVTTMAAASARVLEAVGQAGLDRALVCGLSMGGYVAFELWRRSPGLVLGLVLANTR
                     SEADPEEGAARRRALAERLRAEGSAFLVEDPPPLLSPNASPELRARVRDLIADQPAEA
                     IAAASLGMATRPDSSGDLPRIDVPTLLITGSEDRLIPPEVTSAMARVIPGAGLEVIEG
                     VGHLSNLEAPDRFTDLLREQAARCGLG"
     gene            2093..>2443
                     /locus_tag="VE646_11455"
     CDS             2093..>2443
                     /locus_tag="VE646_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZD80640.1"
                     /translation="MVDAERFTSRYPFALDPFQLHAVEALSAGDSVLVAAPTGSGKTV
                     VAEFAVERALRLEGKAFYTTPLKALSNQKFGDFVARYGAAKVGLLTGDNSINPEASVV
                     VMTTEVLRNMLYEKS"
BASE COUNT          320 a          844 c          882 g          397 t
ORIGIN      
        1 tgttcatcgg ggccgtcgac gcggtcgtca tcaagctgaa ggtggtggtg ttcctcggcc
       61 tgctgatcgc gctgccggtg gtcctgtacg agctgtggtc gttcatcgtg cccggcctca
      121 ccaagcatga gcggcggatg gcgatcccgt tcgtcctgtc ctcggtgatc ctgttcggga
      181 tgggagcggc gctggcctac ttcacgcttc ctcgggccct cggcttcctg ctcgggttcg
      241 ccggtccggg gttcacgccc ctcctcaccg gggacaagtt cctgggcttc gtcatgctcg
      301 tcgcgttggc gttcgggctg tcgttcgagt tccccatcgt gctggtcttc ctggcgatgg
      361 tgggggtgat ctccagccgg cagatgaagg ggtggcggcg gggcgcgatc gtgttcatcg
      421 ccgtgttcgc ggccgtgatc acgccgagcc aggacccgta cacgatgctg gcgatgatgc
      481 tgcccatggt gctgttctac gagggcgcta tcatcgtttc ccgcctcctc ggtcattagg
      541 agctcgcgta tggcgatcaa gggcaagagc aagacccgga gccggcgcgc ggtgaccccc
      601 ggccctcggc cggtctacgt gccggtcaag aagcccctgt acgcccggcg cgagatccag
      661 gtggccgtgg tcgtcctgct ggtgatcgcc gccgccggag ggatctggta cggggtggcc
      721 cacagccgca cccaggcccg ggcacgcgag cagcagcggc tgatgcgcac cgtggcctcg
      781 gcctaccagg tggaggtcaa ccaggccctg tccggggtcg gccagcccac gcccccggtg
      841 gggttcagcg cgatgcccgg gctgaagccg gacatccagg gactgcggaa gggatcggtg
      901 gacccgagca aggccaccgc cgatgccggg tccttcgctc agacggcgtc caaggccgcc
      961 gatgccttcg acaagatcga cgccacctcc ctggtgagcg gcaaaggcat caccgaccag
     1021 gtcttcgtcc tgaacctcct caactcccgc acccgcatgg aggaggccct gaagctctac
     1081 gggaacgccg cccggctgct cggggacgcg gccacggcca ccgggggaac gcgcaccgag
     1141 ctgctgcaac gggcctcctc cgtcaccgac gtcgcctcgg gcctgttcgc cgaggggtac
     1201 aacgactacc tgaacgcgca ggacgcggcg gggatcctcc agcccaccct gccctcgagc
     1261 ggatcgccgg tgggcgggtc gccgctgggc gggggctcct gagcgtgagc gacggtctcc
     1321 tgctcctgca cgccttcccg ctcgacgccc ggatgtggga gccgcaggtc tccaagctcg
     1381 gatccgagct gccggtggtg gcgccggacc tgccgggctt cggggggagc cctccggccg
     1441 ccgatgtcac cacgatggcg gcggccagcg cgcgcgtgct cgaggcggtg ggccaggccg
     1501 gcctggatcg ggccctggtc tgcggactgt cgatgggggg gtacgtcgcg ttcgagctgt
     1561 ggcggcgatc cccggggttg gtcctgggcc tggtcctggc caacaccagg tcggaggcgg
     1621 acccggagga gggggccgcg cgacggcgag cgctggccga gcgcctgcgc gccgaaggga
     1681 gcgcgttcct ggtggaggac ccgccgccgc tgctctcccc gaacgcctcg ccggagctcc
     1741 gggcacgggt ccgggacctg atcgccgatc agcccgccga ggccatcgcc gcagcctcgc
     1801 tggggatggc cacccggccc gattccagcg gggacctgcc tcggatcgac gtgccgacgc
     1861 tgctgatcac gggctccgag gatcggctga tcccgccgga ggtcacctcg gcgatggcac
     1921 gggtgatccc cggggccggg ctggaggtga tcgagggggt ggggcacctc tccaacctcg
     1981 aggcgcccga tcgcttcacg gacctgcttc gggagcaagc cgcccgctgc ggcctgggct
     2041 gagccttcgc cccggctccg ggctcggtcc ggggcgcccg tagactggct gcatggtcga
     2101 tgccgagcgc ttcacctcgc gctacccatt cgcgctggat ccgttccagc tccacgccgt
     2161 ggaggctctg tccgcgggcg actcggtgct ggtggccgcg cccaccggct cgggcaagac
     2221 cgtcgtagcc gagttcgccg tggagcgggc cctccggctc gaggggaagg ccttctacac
     2281 cacccctctg aaggccctgt cgaaccagaa gttcggcgac ttcgtggccc ggtacggggc
     2341 ggccaaggtg gggctgctca ccggtgacaa cagcatcaac ccggaggcgt cggtggtcgt
     2401 gatgaccacc gaggttctgc gcaacatgct ctacgagaag agc
//
LOCUS       DASQQQ010000301         3129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_220483, whole genome shotgun sequence.
ACCESSION   DASQQQ010000301 DASQQQ010000000
VERSION     DASQQQ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220483"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="VE646_11460"
     CDS             complement(<1..168)
                     /locus_tag="VE646_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018791670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HZD80641.1"
                     /translation="MAGRGRSMAELVLTEEEREVLQRWARRPTSAQALALRCRIVLAC
                     AEGKSNQQVAAD"
     gene            complement(247..621)
                     /locus_tag="VE646_11465"
     CDS             complement(247..621)
                     /locus_tag="VE646_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HZD80642.1"
                     /translation="MDDHELAELDYCYLTTTGRASGRPHTIEIWFALSGDTLYVLSGG
                     GDASDWVKNVAAHPTVGLRLGDLDMICRGRVVSDPAEDELARRLLLDKYVPRSSDDLT
                     EWGRTALPIAVDLPPVADQAPA"
     gene            complement(730..930)
                     /locus_tag="VE646_11470"
     CDS             complement(730..930)
                     /locus_tag="VE646_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80643.1"
                     /translation="MDARRDTEGWVVLVLDPDGREVSRRPCGDETEAWTYASTVRQHV
                     YWLSEERFRTYYRLPSAGEETD"
     gene            complement(962..1243)
                     /locus_tag="VE646_11475"
     CDS             complement(962..1243)
                     /locus_tag="VE646_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80644.1"
                     /translation="MKIKGTCRHCGREFLAEQVVENGGRCPWDGRPFQADYAVVLVDS
                     LRDAGNAGNTLENALEKIADLDPEFVLDVDSVLGQIRAHLERLESGHGR"
     gene            complement(1240..1626)
                     /locus_tag="VE646_11480"
     CDS             complement(1240..1626)
                     /locus_tag="VE646_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZD80645.1"
                     /translation="MKVLLMSPDPASREAMQLAVASIERRSGAPIRFAEAEDGQEALR
                     RAFRDPPDVIVAEEIASRMGAFALAKDLRGAERPFRGAIVILLERAQDVWLADWSGAD
                     AWFVKPVDPFQLADRLQELVRTKEIA"
     gene            1725..2405
                     /locus_tag="VE646_11485"
     CDS             1725..2405
                     /locus_tag="VE646_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HZD80646.1"
                     /translation="MEATWRAAAAELVASGTFVFLAAGSVIVAGPSGAGLVGVALASG
                     FAFAVAVALARPTSGGHVNPVVTIALWVEGRISSLRAASFVLAQLSGAVIGSLLLRAG
                     VPEPMWQAAHLGTPLPAADLGAGPAVLLEAVLGFGLALAFLAVPGVGGSIPDLGPLLV
                     GLVLAADVLAAGPLTGAGVSPARALGPGLVSGTWHGWWVYWVGPPAGGVLAALVHRSI
                     FPGRSLDR"
     gene            2467..>3129
                     /locus_tag="VE646_11490"
     CDS             2467..>3129
                     /locus_tag="VE646_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZD80647.1"
                     /translation="MKDIYLDHGSATPLHERSRRALVGALDAFGDPLRLHARGRLART
                     ILDDARAEVSAAIGAQPDEIVFTSGGTEAVALAIWGGVRPIRELGDRVVVSAVEHPAV
                     GGVTHTLEVDGIEVVMVGVDGSGQVDLDRFAAEVRRPGTLLASLQHANHEVGTIQPIA
                     EAGRLCREAGVLFHTDACQTVGRLPISTQDLEVDLLSLSAHKFGGPAGVGALFVRRGV
                     PVA"
BASE COUNT          419 a         1143 c         1078 g          489 t
ORIGIN      
        1 atcggccgcg acctgctggt tgctcttgcc ctcggcgcag gccagcacga tcctgcaccg
       61 cagcgccagc gcctgcgcgc tcgtgggtcg ccgagcccac cgctggagca cctcgcgctc
      121 ctcctcggtg agcaccaact ccgccatgga ccgacctcgt cccgccatcg tcgcctccca
      181 tcgtcgccga caaccgatga gaacacgacc catggcatca ttccagtgga cttacgactc
      241 aggacactag gcgggagcct ggtcggccac cggaggcagg tcgaccgcga tcggcagggc
      301 ggtcctcccc cattcggtca ggtcgtcgga cgagcgcggg acgtacttgt ccagcagcag
      361 ccgccgggcc agctcgtcct ccgcgggatc ggagaccacg cggccccggc agatcatgtc
      421 caggtccccc agccgcaacc ccaccgtggg atgagcggcg acgttcttca cccagtcgct
      481 ggcgtctccg ccgcccgaca gcacgtacag cgtgtcgccg gacagcgcga accagatctc
      541 gatcgtgtgg gggcgtcccg acgcccgtcc ggtggtggtc aggtagcagt agtcgagctc
      601 ggcgagctcg tgatcgtcca cgttagcgga tggcccacac caccaggccg acgaccacca
      661 cgatcgcggc caggaggtac agcagatccc cccgcttgac cttcccgggc tgcatggcgt
      721 tgaagcccat cagtccgtct cctccccagc cgagggcagc cggtagtacg tacggaaccg
      781 ttcctccgag agccagtaga catgctggcg taccgtggag gcataggtcc aggcctcggt
      841 ctcgtccccg caggggcgcc tggagacctc ccgaccgtcc ggatcgagca cgagcacgac
      901 ccacccctcc gtgtcccgcc tggcgtccac gcggtagcgg ttctcactca ggtcgacgag
      961 gtcagcgtcc gtggccgctc tccaaccgct cgaggtgcgc tcggatctgc cccaacacgg
     1021 agtccacgtc gagcacgaac tccggatcca ggtcggcgat cttctccagc gcgttctcca
     1081 gcgtgttccc ggcgttgccc gcgtcgcgca gggagtccac gagcaccacc gcgtagtcgg
     1141 cctggaacgg ccgcccgtcc caggggcacc gacctccgtt ctcgaccacc tgctcggcca
     1201 ggaactcccg cccgcagtgt cgacatgtgc ccttgatctt cacgcgatct ccttggtccg
     1261 cacgagctcc tgcagacgat cggccagctg gaaggggtcg acgggcttca cgaaccaggc
     1321 atccgcaccc gaccagtcgg ccagccagac gtcctgtgcc cgctccaaca ggatcacgat
     1381 ggcaccacgg aacggccgct ccgccccccg gaggtccttg gccagcgcga aggcccccat
     1441 cctcgaggcg atctcctccg ccacgatgac gtccggcggg tcccggaagg cgcggcggag
     1501 ggcctcctgt ccgtcctccg cctccgcgaa ccggatcggc gcgccggaac gccgctcgat
     1561 gctggccacg gcgagctgca tcgcctcgcg tgaggccgga tcgggggaca tgagcaggac
     1621 cttcatcgcc gacgcatcgt atcaacagga gccctttgtc cctcgccatc cgttcctcta
     1681 tggtccgggc gtccggcgaa agggctcgtc gagcaggagg tgcggtggaa gcgacctgga
     1741 gagcggcggc cgccgagctg gtggcctcgg gaacgttcgt cttcctggcg gcgggatcgg
     1801 tgatcgtggc aggaccctcg ggcgccggcc tggtgggggt ggctctggct tccgggttcg
     1861 cgttcgccgt ggccgtcgcg ctggcccggc cgacctcggg tgggcacgtg aatccggtcg
     1921 tgacgatcgc cttgtgggtc gaggggagga tctcgtccct ccgcgccgcc tcgttcgtcc
     1981 tggctcagtt gagcggggcg gtgatcggct cgctgctgct ccgggccggt gttcccgaac
     2041 cgatgtggca ggccgcccat ctggggacgc ccctgccggc agccgacctc ggagccggcc
     2101 ccgcggtcct gctcgaggcg gtcctggggt tcggactcgc cctcgccttc ctggccgttc
     2161 ccggggtggg gggatcgatc ccggacctgg gccccctcct ggtcgggctg gtgctggccg
     2221 ccgatgtcct cgcggccggt ccgctgaccg gcgccggtgt gagcccggca cgggccctcg
     2281 ggccgggact ggtctcgggc acgtggcacg gctggtgggt ctactgggtg ggccctcccg
     2341 ccgggggggt ccttgccgcg ctcgtccacc gatccatctt ccccggccgg tccctcgacc
     2401 gctgacggga gcggggtcgg actatcatga cccccgtccg accgctcccc ggagtcccct
     2461 gaggccatga aggacatcta cctggaccac ggctcggcga cgccgctgca cgagcgctcc
     2521 cggcgagccc tcgtgggcgc cctcgacgcc ttcggggatc ccctgcgcct gcacgcgagg
     2581 ggacggctcg cgcgcacgat cctggacgac gcgcgggccg aggtctcggc ggcgatcggg
     2641 gcccaaccag acgagatcgt cttcacctcc ggggggacgg aggcggtggc gctggccatc
     2701 tggggcgggg tgcgtcccat ccgcgagctc ggcgaccggg tggtggtgag cgccgtggag
     2761 catcccgccg tgggcggggt gacgcacacg ctcgaggtgg acgggatcga ggtcgtgatg
     2821 gtgggggtgg atggatccgg ccaggtggac ctagaccggt tcgccgccga ggtgcgccga
     2881 ccgggaaccc tgctcgcatc cctccagcac gcgaaccacg aggtcgggac catccagccg
     2941 atcgccgaag ctggtcggct gtgccgggag gccggggtgc tgttccacac cgacgcctgt
     3001 cagaccgtgg gccgcctgcc gatctccacg caggacctag aggtggacct gttgtccctg
     3061 tccgcccaca agttcggggg cccggccggg gtgggcgccc tgttcgtgcg gcgaggcgtc
     3121 cccgtggcc
//
LOCUS       DASQQQ010000302         3272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_81873, whole genome shotgun sequence.
ACCESSION   DASQQQ010000302 DASQQQ010000000
VERSION     DASQQQ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81873"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VE646_11495"
     CDS             complement(<1..657)
                     /locus_tag="VE646_11495"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZD80648.1"
                     /translation="MAAIMILERDVRAAGLRHPAAVNLDAVEHRRSQLWTISFVVMAV
                     LAVGTTFLSESIPRLADDASVGTAPAVRLGLLALVAGLVLYVSEKERHLRRLTRLLIQ
                     GSASATVAVAPGRDALTGLLDRDAFLSQLSAAIDASREHGESLALLLVDVDRFREINA
                     RHGQRAGDLLLQELSRRIRSAAPTAAGARLRSDEFGLLTPGATSSEARDLARRLSQVL
                     R"
     gene            729..1271
                     /gene="rfbC"
                     /locus_tag="VE646_11500"
     CDS             729..1271
                     /gene="rfbC"
                     /locus_tag="VE646_11500"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HZD80649.1"
                     /translation="MAKIIPSQVIHGVVVVELVPHGDARGVFVETYRREWIPGGAEMV
                     QANRADRCAGSVTGLHYHPNQADYWYVTSGAARVVLHDLRRSSPTTGATLTMDLGRSP
                     YEHTGIYIPPGVAHGFGALTDITLTYMVSHYYDTAEELGVAWDDPDIGADWGVDDPVL
                     TERDRSNPRKADIPAERLPR"
     gene            complement(1333..1725)
                     /locus_tag="VE646_11505"
     CDS             complement(1333..1725)
                     /locus_tag="VE646_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZD80650.1"
                     /translation="METRLLLVLALLALLALGRALYRRWQHAVHTDAPDAPPLPARLL
                     EGAERTWVLFTTPWCASCDAVARELTANDPSGRLVKIDATRDLDLTEAYRIRRAPTVL
                     LADDQGEVRARLVGLEAVRGYVRNPFVL"
     gene            complement(1725..2177)
                     /locus_tag="VE646_11510"
     CDS             complement(1725..2177)
                     /locus_tag="VE646_11510"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HZD80651.1"
                     /translation="MATRGPAPAPIDPRGPRFNQGAIAVLLLAGFVLDWWPMAAIVGV
                     ILLLGAALGPRYGPFIRLYSEAIRPRLAPPDELEDPRAPRFAATVGVVFLAGATLAFA
                     AGTSALGWALALIVAALAGLAAVTGICVGCEVWLFLARRRGVPLSVGA"
     gene            2308..>3272
                     /locus_tag="VE646_11515"
     CDS             2308..>3272
                     /locus_tag="VE646_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZD80652.1"
                     /translation="MQLEMLGQAGVLGGALADVAATRERARRARLRRLAVVLAVPALW
                     LWVRMLSGNPVHFGAPTLGPEALIWLPGLFLVLLFGLMLAGPLLGAGRSPHVMYRPSE
                     IDITLDDVKGAATVREEVVRTLNLFLAHKTFREGMGGTPRRAILFEGPPGTGKTYVAK
                     ALAREAGVPFLFVSSSAFQSMYYGQTNRKIRSFFRALRHYARTEGGAIGFIEEIDAIG
                     ASRNGMGSSTHREGIAGVVNELLIQLQSFDEPTRRQRINGWLVDRINRYLPSHRQLRK
                     DRPQHANVLVVGATNRASDLDPALLRPGRFDRVVHFGLPNRRERR"
BASE COUNT          498 a         1123 c         1205 g          446 t
ORIGIN      
        1 gcggaggacc tgcgagaggc gccgggccag gtcgcgtgcc tcgctgctgg tcgctccggg
       61 ggtgaggagg ccgaactcgt cgctgcggag gcgggctccc gcggcggtcg gagcggcgga
      121 ccggatgcgc cgcgacagct cctgcaggag gagatcgccg gcccgctggc cgtggcgggc
      181 gttgatctcg cggaatcggt cgacgtcgac cagcagcagg gcgagcgact cgccgtgctc
      241 gcgggaggcg tcgatcgccg cgctgagctg gctgaggaag gcgtcgcggt cgaggagccc
      301 cgtcagggcg tcacggcccg gagcgacggc cacggtcgcg ctggccgacc cctggatgag
      361 cagccgggtg agccggcgga ggtggcgttc cttctcggag acgtagagca cgagcccggc
      421 gacgagcgcg aggagcccga gccggacggc cggggccgtg ccgacggacg catcgtcggc
      481 caggcggggg atgctctcgg agaggaaggt cgtgccgacg gccaggacgg ccatcacgac
      541 gaacgagatc gtccacagct ggctgcggcg atgctcgacg gcgtcgaggt tcacggctgc
      601 gggatggcgg agcccggcgg cgcgcacatc ccgttcgagg atcatgatcg cggccacgac
      661 gcgaacatcg gcacgcgacc atgggccgct tgacctagat gtggccgatc ggcgcgtagt
      721 gtgccccggt ggcgaagatc atcccctcgc aggtgattca cggagttgtg gtggtcgagc
      781 tcgtgccgca cggcgacgcc cgcggcgtct tcgtcgagac gtaccgcaga gagtggatcc
      841 ccggcggtgc cgagatggtg caggccaacc gggccgaccg gtgcgccggc tcggtcaccg
      901 ggctccacta tcacccgaac caggccgact actggtacgt cacgtcaggt gcggcccggg
      961 tggtgctcca cgatctgcgc cggagctctc ccaccaccgg cgccacgctg acgatggatc
     1021 tcggccggtc accgtacgag cacaccggca tctacatccc ccccggcgtg gcccatgggt
     1081 tcggtgctct caccgacatc acgctcacgt acatggtgag ccactactac gacacggccg
     1141 aggagttggg ggtggcgtgg gacgaccccg acatcggggc cgactggggt gtcgatgatc
     1201 cggtgctgac cgaacgcgac cggtcgaatc cccggaaggc cgacatcccc gccgagcgcc
     1261 tgcctcgcta gccgctcacc tctccccccc tcgttctgtg aggatttttc cgtgcagagc
     1321 acggaaaaat cctcacagaa cgaatgggtt gcggacgtac ccccggaccg cttcgagacc
     1381 cacgaggcgg gctcggacct cgccctgatc gtcggccagg agcacggttg gggcccggcg
     1441 gatccggtac gcctcggtca ggtcgaggtc gcgggtggcg tcgatcttca cgagccggcc
     1501 gctgggatcg ttggcggtca gctcacgggc gacggcgtcg caactggcgc accagggcgt
     1561 ggtgaacagc acccacgtgc gttcggcccc ctccaggagc cgggccggca gcggcggagc
     1621 gtcgggcgcg tcggtgtgca cggcgtgctg ccagcgccgg tagagggccc ggcccagggc
     1681 gaggagcgcg aggagggcga ggaccagcag caggcgggtc tccatcaggc ccccacgctc
     1741 agcggtacgc cccgccggcg ggcgaggaac agccacactt cgcacccgac acagatgccg
     1801 gtgaccgcgg ccagcccggc gagcgcggcc acgatcagcg ccagtgccca gccgagggcg
     1861 gacgtgccgg cggcgaaggc cagcgtggcc ccggccagga acacgacgcc gacggtggcg
     1921 gcgaagcgag gcgcccgggg atcctcgagc tcgtcggggg gtgccagccg gggccggatc
     1981 gcctcggagt agaggcggat gaagggcccg taacggggcc cgagcgccgc cccgaggagg
     2041 aggatcacgc ccacgatcgc cgccatcggc caccaatcga gcacgaatcc ggcgagcagg
     2101 agcaccgcga tcgccccctg gttgaaccgg gggcctcggg gatcgatcgg tgccggtgcg
     2161 ggtccccttg tagccacggg ggcgagtcta atcccgatcg ggccagtcgg gaattccgaa
     2221 gttgggccgg aaggaggatt tccctggcca gggaaagaag gtactgtcag cactcaacgt
     2281 ggtcaccaga tacggagcgt gaggaacatg cagctcgaga tgctcggtca ggcgggggtg
     2341 ctcggagggg cgctggccga cgtcgccgcc actcgggagc gggcccggcg ggcccggctc
     2401 cggcgcctgg cggtggtgct ggcggttccg gccctgtggc tctgggtgcg gatgctgagc
     2461 ggcaatcccg tccacttcgg ggccccgacc ctcggcccgg aggcgctgat ctggcttccc
     2521 ggtctcttcc tggtcctgct cttcggcctc atgctggccg gcccgttgct gggtgcaggt
     2581 cgctccccgc acgtcatgta ccggccgagc gagatcgaca tcacgctcga cgacgtgaag
     2641 ggcgcagcca ccgtccggga agaggtggtg cggacgctga acctgttcct ggcccacaag
     2701 acgttccgcg aggggatggg cggtactccc cggcgggcga ttctgttcga gggcccgccc
     2761 ggtaccggca agacctacgt ggccaaggcg ctggcgcgcg aggccggggt gccgttcctg
     2821 ttcgtgtcct cgtccgcctt ccagtcgatg tactacgggc agaccaaccg caagatccgc
     2881 tccttcttcc gggccctgcg tcactacgcc cgcaccgagg gcggggccat cgggttcatc
     2941 gaggagatcg acgccatcgg cgccagccgc aacggcatgg gcagctccac acaccgggag
     3001 ggcatcgccg gggtcgtgaa cgagctgctc atccagctgc agtccttcga cgagccgacc
     3061 cgccgccagc ggatcaacgg ctggctcgtc gaccgcatca accggtacct gccgtcccac
     3121 cgccagctgc ggaaggaccg cccccagcac gccaacgtgc tcgtcgtggg ggcgaccaac
     3181 cgcgcatccg acctggatcc cgccctgctc cgcccgggcc gcttcgaccg ggtggtccac
     3241 ttcggcctcc ccaaccggcg ggagcgccgc ga
//
LOCUS       DASQQQ010000303         2912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_20460, whole genome shotgun sequence.
ACCESSION   DASQQQ010000303 DASQQQ010000000
VERSION     DASQQQ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20460"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VE646_11520"
     CDS             <1..416
                     /locus_tag="VE646_11520"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZD80653.1"
                     /translation="AARRMFAGLPVVAVATLNPDGSPHVVPLWFVWPDDAIFMSTRRD
                     SRTWRNAAADPRVALTVDVGRGWTELAGVSVSGAVELLTPDRPTMRKPISAWHEKYRS
                     LLAGEGFARFTEEVAALGFLRLVPERLVGWDHARR"
     gene            534..1790
                     /locus_tag="VE646_11525"
     CDS             534..1790
                     /locus_tag="VE646_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HZD80654.1"
                     /translation="MARPSTAPGTADVLVVGAGPGGSATAYHLARHGLDVLLVERSAF
                     PREKVCGDGLTPRGVKALTRMGIDPDDPGFQRHRGLRIYTRRVTLELPWPTLEDYPNY
                     GLTRTRDDFDELLARRAEKAGATLLEGTEAQQPIVEDGWVRGALVRSTGDGDAGTEVR
                     ARFVVAADGASSRFGGRVGIRRDPSRPLGIAARRYYRTPRDPGPWLEVWFDLWDGDAI
                     MPGYGWLFPLPDGRVNLGAGLMNTFRNFKNLSAQRVFDAFVAMLPPAWELTEDTADGR
                     VLSGPLPMGMNRMPQAVPGMLVVGDAAGIVNPFNGEGIAYAMESGELAAELLYDALVR
                     DRPALAQLYPTLLRQRYGRYYTAGRGFVRAMGHPAAMRIMTDYGIPRPWLMRFSLRML
                     GNLTDGPGGDGYDRVMDTLVRLMPAS"
     gene            1804..2160
                     /locus_tag="VE646_11530"
                     /pseudo
     CDS             1804..2160
                     /locus_tag="VE646_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
     gene            2237..2731
                     /locus_tag="VE646_11535"
                     /pseudo
     CDS             2237..2731
                     /locus_tag="VE646_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
BASE COUNT          370 a          973 c         1098 g          471 t
ORIGIN      
        1 cggccgcccg gcggatgttc gccggtctgc ccgtcgtggc ggtggccacc ttgaacccgg
       61 acggctcccc ccatgtggtg ccgctgtggt tcgtctggcc cgatgacgcc atcttcatgt
      121 ccacccgccg tgactcccgg acctggagga acgcggcggc cgacccacgg gtcgccctca
      181 ccgtcgacgt gggtcgggga tggacggagc tggccggggt cagcgtgtcg ggagcggtgg
      241 agttgctcac gcccgatcga ccgacgatgc gcaagcccat ctccgcgtgg cacgagaagt
      301 accggtccct cctggccggg gaggggttcg cccggttcac cgaggaggtg gccgcgctgg
      361 gcttcctccg gttggtgccc gagcgtctgg tgggctggga tcacgctcga cgctgaggtt
      421 cggttgcccg accgcgggtg ccgttctagg ctgtgccaca ggctttgtga agctcttcac
      481 gagccgatag catcatttgt gaaacttttc acaagcggtg ggggaccgat atcgtggcac
      541 gtccgtcaac ggcacctgga acggccgacg tcctggtggt gggtgcgggt ccgggcggtt
      601 cggccactgc gtatcacctg gcccgccacg ggctcgacgt actgctcgtg gagcgctccg
      661 ccttcccccg ggagaaggtc tgcggggatg ggctcacccc ccgcggcgtg aaggcgctga
      721 cccggatggg gatcgacccg gacgatccgg ggttccagcg gcaccgcggc ctgcggatct
      781 acacgcgccg ggtgaccctc gagctgccct ggcccacgct ggaggactac ccgaactacg
      841 ggttgacgcg gacccgggac gacttcgacg agctcctggc ccggcgggcg gagaaggccg
      901 gtgccaccct gctggagggg acggaggcgc agcagcccat cgtggaggac ggctgggtgc
      961 ggggcgccct ggtgcgctcc acgggcgacg gcgacgccgg gaccgaggtc cgggcccggt
     1021 tcgtggtggc ggccgacggc gcgtcctcgc gcttcggggg ccgggtgggg atccggcgcg
     1081 accccagccg tcccctgggc atcgccgcgc gccgctacta ccggaccccc cgggaccccg
     1141 gtccctggct ggaggtgtgg ttcgacctgt gggacgggga cgcgatcatg cccggctacg
     1201 gctggttgtt cccgctgccc gacggccggg tcaacctcgg cgccggcctg atgaacacgt
     1261 ttcgcaactt caagaacctg tccgcccagc gggtgttcga cgcgttcgtc gccatgttgc
     1321 cgccggcctg ggagctgacc gaggacaccg cggacggccg ggtcctctcc ggccccctgc
     1381 ccatgggcat gaaccggatg ccccaggcgg tgcccggcat gctggtggtg ggggacgccg
     1441 cgggcatcgt gaacccgttc aacggggagg ggatcgcgta cgccatggag agcggtgagc
     1501 tggccgccga gctgctgtac gacgctctgg tgagggatcg tcccgccctg gcccagctgt
     1561 acccgacact gctccgtcag cgctacggcc gctactacac ggccgggcgc gggttcgtgc
     1621 gagcgatggg tcaccccgcg gccatgcgga tcatgacgga ctacggcatc ccgcggccct
     1681 ggctgatgcg gttctcgctg cggatgctcg gcaacctgac cgacgggccc ggcggcgacg
     1741 gatacgaccg ggtgatggat acgctggtgc ggctgatgcc ggcatcctga ggaggaggat
     1801 cgcgtgctgt ccgactacct gccgatcggg gtcctggcgg ccctggcgct ggtgttcgcg
     1861 gtggcctcgc tggcggtttc ctccctgctc cggcctcggc gccccacccc ggccaagctc
     1921 gccccgtacg agtgcgggat cgagccgcag cgcctaccgc gcggcgagcg gttcagcgtg
     1981 aagttctacg tggtcgcgat gctgttcatc atcttcgata tcgagacgat cttcctcttc
     2041 ccctgggcgg tggggttccg ccgtctgggg ctgttcgggc tggtggagat ggcggtgttc
     2101 atcgcgctgg tcttcgtggc ctacctgtac atctggcgca gcggcggtct ggattgggac
     2161 cgcccggtgc ggcggaccgc tcggaccggc gtggtgggcg ccagcgaggg gatgcccgag
     2221 gaccgggggg tgctgcatgc ccgttgagcg cctgtccggc ttccaggtcg gcaagacgga
     2281 gaccgagggg gaggaggagc tccgcagggg gatcttgctc accaccctgg acaaggcggt
     2341 gcgctgggcc aggaagcagt cgatgtggcc ggtcaccttc gggctggcct gctgcgcgat
     2401 cgagatgatg gccaccggcg ccgccgacta cgacctgtcc cggtggggga tggagctgtt
     2461 ccgggcctcg ccccggcagg ccgacctgat gatcgtggcc ggccgggtct cgcagaagat
     2521 ggccccggtg ctccgcaccg tctacgacca gatgaccgag ccgaagtggg tcatctcgat
     2581 gggggtgtgc gcctcggccg gggggatgtt cacgaactac gccatcgtgc agggcgtgga
     2641 caccatcgtc ccggtggaca tctacgtccc gggctgtccc cccaagcccg agatgctgat
     2701 gtacgggatc ctccgcctgc acgagcaggt gcggacgcag ggcgagcgcc cgtgacggcg
     2761 gccgagctgg ccgaacgggt ccgggcccgg ggcggcctgg actggaacct ggccggcctg
     2821 agggacgagc tggcccgcgg tcgctccgcc gtctacccgg agccggagcc gcccgccgcg
     2881 tccggcggcg agcgggaggt ggcccgtgcc gg
//
LOCUS       DASQQQ010000304         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_82215, whole genome shotgun sequence.
ACCESSION   DASQQQ010000304 DASQQQ010000000
VERSION     DASQQQ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82215"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VE646_11540"
     CDS             <1..306
                     /locus_tag="VE646_11540"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZD80655.1"
                     /translation="LDADGRYHLALLELAAGQPGAARAEADTVLQSEPRHLLALYVAA
                     EAERALGQPDRAAALYRRYLDAYDPDSLGSRPEYLQHQPVLPRAAEEARAFLRTMGR"
     gene            362..796
                     /locus_tag="VE646_11545"
     CDS             362..796
                     /locus_tag="VE646_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HZD80656.1"
                     /translation="MKSHTAYRTFETKKRQEIIHLTPWVEEEVSKSGIQEGLCLVSAM
                     HITASVFVNDNEPGLHQDIMDWLEGDVAPWGAHPARGDDYLHNRTGEDNGAAHLRNLT
                     LGHEVILPVTKGKLDLGPWQRVFYGEWDGRRPKRLVLKMIGA"
     gene            complement(1113..1862)
                     /locus_tag="VE646_11550"
     CDS             complement(1113..1862)
                     /locus_tag="VE646_11550"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HZD80657.1"
                     /translation="MTPKRIIVTLPLEGRGALSVEPALADPPDPRDPVAVVETLVQDG
                     ADEFLLTTDPSAPPPPDILARTVERLTAVALPFAVRAEPTSAAAASRLLEAGAARIVI
                     GASGLADPDFIARLVDRFGSASVAVSLTAAREDGTWRVQEDGGIATEWQAVTWARVAE
                     AQGAGELWVRAPAPDGGPYDLGLLESVTSALHIPVVAAGEARSVQDLFDALMIGDSDG
                     VAAAGLFLSGSMTVEMARADLAEHGLPVRRD"
BASE COUNT          318 a          693 c          717 g          272 t
ORIGIN      
        1 ctggacgcgg acggccggta ccacctggcg ctactggagc tggcggccgg ccagccgggg
       61 gccgcccggg ccgaggcgga cacggtcctc cagtcggagc cgaggcacct gctggccctg
      121 tacgtggccg cggaggcgga gcgggcgttg ggccagccgg accgggcggc ggcgctctac
      181 cggcggtatc tggacgccta cgaccccgac tcgctgggga gccggccgga gtacctacag
      241 catcagcccg tcttgccgcg ggccgcggag gaggcccggg ccttcctgcg gacgatgggg
      301 cgttgagccg gcgggcgacc ggccccgagc cacggacgga ccacggacac aaggagccgg
      361 gatgaagtcg cacacggcgt atcgcacgtt cgagacgaag aagcgtcagg agatcatcca
      421 tctgaccccc tgggtggagg aggaggtctc gaagagcggg atccaggagg gcctgtgcct
      481 ggtgagcgct atgcacatca cggcgtcggt cttcgtcaac gacaacgaac cgggactgca
      541 ccaggacatc atggactggc tggaggggga cgtggccccc tggggggcac acccggcccg
      601 gggcgacgac tacctgcaca accggaccgg cgaggacaac ggggccgctc acctgcgtaa
      661 cctgaccctg ggacatgagg tgatcctgcc ggtcacgaag gggaagctgg atctggggcc
      721 gtggcagcgg gttttctacg gcgagtggga cggacggcgg ccgaagcggc tcgtgctcaa
      781 gatgatcggc gcctgaaggc gcgacacctg tcgtggcctc ggcgcacggt gtaaattacg
      841 gccggagagc agtcgagcca cgcgaacgat gcagaatcga gcgaaacatc cggtcgtcga
      901 gcttagggag tggctttatc ccgacaacat ccgtcctggc cagcgggtcc gctgtcagat
      961 gccgggatgt cacgccctgc ttggccggac cggccaccac ctgcgtttcc ggtcgctacg
     1021 caagagcgat gcggctctgc cgcccgagtg ggtgggatgg cactgcagtc gctgcggtca
     1081 cctgaacgtg tactgcccgc tcgactgacc gctcagtcac gccgcacggg gaggccgtgc
     1141 tccgccagat cggcccgcgc catctccacc gtcatcgatc ccgaaagaaa gagcccggcc
     1201 gcggcgacgc cgtccgagtc accgatcatc agcgcatcga acaggtcctg caccgaccgg
     1261 gcctcgccgg cggccacgac gggtatgtgc aaggcggacg tcaccgactc cagcagaccg
     1321 aggtcgtagg gccctccatc cggcgccggg gcccggaccc acagctcgcc cgccccctgt
     1381 gcctcggcca cccgcgccca ggtgacggcc tgccactcgg tggcgattcc gccgtcctcc
     1441 tggacccgcc acgtcccgtc ctcccgggcc gccgtcaggg acaccgccac cgatgcggag
     1501 ccgaagcgat cgaccagccg cgcgatgaag tcgggatcgg ccaggccgct cgcaccgatg
     1561 acgattcggg ccgcaccggc ctccagcagc cgggaggccg cggccgcgct cgtcggctcc
     1621 gcccgcacgg cgaacgggag cgccacggcg gtcagtcgct cgacggtgcg ggcgaggatg
     1681 tcgggcggcg gcggcgcgga ggggtccgtc gtcaggagga actcgtcggc accgtcttgg
     1741 acgagggtct ccacgacggc tacggggtca cgagggtccg gcgggtcggc cagagccggc
     1801 tcgacgctca gggcgcctct cccctcgagg ggcaacgtga cgatgatgcg cttcggggtc
     1861 acggcctcga ctccttcggc cccgccgtcg gcggacgaag agtcagtctt cctgctcctc
     1921 ctggggcgtc tcctcgatct ccagcgcgac gtttctccgg atccggtggc tgaacagcgt
     1981 gatctcgttg aaacccgccc
//
LOCUS       DASQQQ010000305         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_20612, whole genome shotgun sequence.
ACCESSION   DASQQQ010000305 DASQQQ010000000
VERSION     DASQQQ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20612"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..>2085
                     /locus_tag="VE646_11555"
     CDS             195..>2085
                     /locus_tag="VE646_11555"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD80658.1"
                     /translation="MMEATLRDLWLAARAIRRSPAISVAVILTLAVGLGATLAIVSVV
                     DGVLIDPVPVPDAHRVVRVFEAWDAETPYSETAYPTLQDYRRARANLEGPTGFASLDV
                     GIRADDLTDRVSAGLVTGDYFQVLQVPAVLGRTLQPADDRLEAPPTAVLGYALWQRMF
                     GGDLAVLGRTVEVSGTPFEVVGVSPEGFRGVTLSAAPELWIPLSHVRQAAAEGLFAAP
                     NVLTTRAFPWIAMVARLPDGATAAAAEAVLDGVAHALRSELGDDSGLPDAPRIVSLRP
                     LAASATLAQRDRLIRFVELLGGVVAMTLVVACLNGATLISARAWTRSRELGVRQALGA
                     RRGRLMWHLLAEGLVMALAGGGAGLGLAFLATRAIRRFALPGGVPLSRVDLSLDGRLV
                     AAALALSTLSALAFGLGPALSQSRRDVGERLREGGRTTSGRSRALPLMVTVQVALSLV
                     LVVGALLFTRTLRAALGTDLGFEPGPLAAVSMGFRGQGYAVDELPDAVERILADVEAS
                     PAVRSAAFAAHVPLAPRTMGLRPVPEGAGEDYSAPVININVVSPHYFETLGVPLLEGR
                     AFEPGDTEDAPDVAVVNEAAAELLWPGERPLGRTFVLLRGLDPVRVVGVARDHKVHAV
                     TDEAVP"
BASE COUNT          230 a          698 c          838 g          319 t
ORIGIN      
        1 ggcccgagat cgtggggctg tgtcgggagc ggcgccacgg tctggggcgg gcctctcccg
       61 cgcgggcggt gaccttctgg gcgagggagc tcggtgcggc ggcacgggcg gtctgggcgg
      121 cctggctgga ccaactgcgg gcccggtccc cgcgtccaac cggagcgacg accaccggag
      181 gacgtgggag gacgatgatg gaggccacgc tacgggacct gtggttggcg gcgcgcgcga
      241 tccgccggag cccggccatc tccgtcgcgg tgatcctcac cctggcggtg gggctcggcg
      301 cgaccctggc catcgtgtcg gtggtggacg gagtgctcat cgacccggtg ccggtgcccg
      361 acgcccaccg ggtggtgcgc gtcttcgagg cctgggacgc ggagacgccg tactcggaga
      421 cggcctatcc cacgctccag gactaccggc gggcccgcgc gaacctcgag ggtccgacgg
      481 gcttcgcgtc gctggatgtc gggatccggg ccgacgacct cacggaccgg gtgtcggcag
      541 ggctggtcac gggggactac ttccaggtgc tgcaggtgcc cgccgtgctg ggccgcacgt
      601 tgcagcccgc tgacgaccgg ctcgaagcgc cgcccacggc cgtgctgggg tacgccctgt
      661 ggcagcgaat gttcggcggg gatctcgccg ttttggggcg aaccgtggag gtgagcggca
      721 cccccttcga ggtggtgggc gtgagcccgg agggcttccg gggggtgacg ttgagcgcgg
      781 ctccggagct gtggattccc ctgagccacg tgcgccaggc cgcggcggag ggcctgtttg
      841 ccgcaccgaa cgtgctcacg acccgggctt ttccgtggat cgccatggta gcccgccttc
      901 cggacggggc cacggcggcc gccgccgagg cggtgctgga tggagtggca cacgcactcc
      961 ggagcgagct gggcgacgac agcgggctcc ccgacgcgcc gcggatcgtg agcctccggc
     1021 ccttggcggc gagtgcgaca ttggcccagc gcgaccgcct gatccgtttc gtggagctgc
     1081 tggggggggt ggtggccatg acgctggtgg tggcgtgcct gaacggcgcc accctgatct
     1141 cggcccgggc gtggacgcgc agccgggagc tgggggtgcg gcaagcgctg ggtgcccgcc
     1201 gaggccgcct gatgtggcat ctcctcgccg aagggctcgt gatggccctg gcaggaggcg
     1261 gcgcgggact ggggctcgcg ttcctggcca cccgggccat ccggcgtttc gcccttcccg
     1321 ggggcgttcc tttgagtcgc gtggacctgt ccctcgacgg ccgcctggtg gccgcggccc
     1381 tggctctgag cacgctgtcg gcgttggcct tcggcctcgg gcctgccctc agccagagcc
     1441 ggcgcgacgt gggcgagcgg ctgcgtgagg gcgggcgcac gacgtccggc agaagccggg
     1501 cgttgccgct catggtgacg gtccaggtgg cgctgtcgct ggtgctcgtg gtgggggctc
     1561 ttctcttcac gcggaccctg cgggcggcgc tgggcacgga cctcggcttc gagcccgggc
     1621 ccctggccgc ggtgtcgatg ggcttccggg gacagggcta cgcggtggac gagctccccg
     1681 acgccgtgga acggatcctg gcggacgtgg aggcgtcccc ggcggtgcgt tcggcggcct
     1741 tcgcagcgca tgtgcccctg gcgccgcgga ccatgggact acggcccgtg cccgaaggcg
     1801 ccggcgagga ctattctgcg ccggtgatca acatcaacgt cgtctcgccg cattacttcg
     1861 agacgctggg ggtgcccctg ctggaaggcc gcgccttcga gcccggcgac accgaggacg
     1921 ctccggacgt ggcggtggtg aacgaggccg cagccgagct gctctggccc ggcgagcgcc
     1981 cgctgggccg gacgttcgtc ctgctgcggg gcctggatcc ggtccgggtc gtgggggtgg
     2041 cgcgggacca caaggtccac gccgtcaccg acgaggccgt gccgt
//
LOCUS       DASQQQ010000306         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_82324, whole genome shotgun sequence.
ACCESSION   DASQQQ010000306 DASQQQ010000000
VERSION     DASQQQ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82324"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1181
                     /locus_tag="VE646_11560"
     CDS             54..1181
                     /locus_tag="VE646_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HZD80659.1"
                     /translation="MDSGTSEDRLIHDWAHPATNAPPRRPMLDDETLRDGLQSPSVQD
                     PPVDVKIRILHLMDSLGIETADVGLPGAGPRQRDAVEALCREIDTARLRIRPNCAART
                     MVVDIQPIADVSQKTGLPVEACIFIGSSPIRQYAEEWDLDHILRHTRQATEFAVREGL
                     DVMYVTEDTVRSSPEHLRVLLTAAIDAGARRVCLCDTVGAAVPEGVINLTRWVRALLE
                     DLGVGERVGNTLMEQLLVNLRLLGWREGDLGRLTEYTELVSQAVGVPIPVNQPIVGRD
                     AFRTATGVHAAAVIKAQRKGQAWLADRVYSGVPAGWIGRHQEIEVGHMSGGSNVQHFL
                     AARGLTATPETVEAVLAAAKRSKRLLTEEEVLQHVRTPASS"
     gene            complement(1255..1809)
                     /locus_tag="VE646_11565"
     CDS             complement(1255..1809)
                     /locus_tag="VE646_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80660.1"
                     /translation="MRSNGMNPSRKAALATAVIFLVGAAAGMAADRVWLGLWPPRAEA
                     ASLTPEGLVRALDLDSTQRARVDAVLDSLQVEMTGAVTAGPDSLRSVARRGRQRLEDA
                     LPPDRRAPFRQWMQAHHARMMQYMGRGMMGPDSGAGPPMTPGGRMGPGMMRPDGGMGP
                     GMMGPDSGRGTGMMGPRGRGRGMR"
     gene            complement(1806..2129)
                     /locus_tag="VE646_11570"
     CDS             complement(1806..2129)
                     /locus_tag="VE646_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80661.1"
                     /translation="MLERYRRPSEAPASAAVSWWQALSLWWRPAVAGAMGLAASIVLV
                     VGLGSGRAPAGPPGAEPDPAALVLSALASGGEPAALWQRAGIEADPLLALLALEPASR
                     EGGGR"
BASE COUNT          331 a          864 c          826 g          312 t
ORIGIN      
        1 ccaccagttc gctcgcgcca acgcccactg aatcgaccgg gacggaggtc agcatggaca
       61 gcgggacgtc cgaggaccgc ctcatccacg actgggcgca ccccgccacg aacgcgccgc
      121 cgcgccggcc catgctcgat gacgagacgc tgcgggacgg gctccagagt ccgtcggtgc
      181 aggacccgcc ggtggacgtc aagatccgga tcctccacct gatggacagc ctggggatcg
      241 agacggccga cgtcgggctg cccggcgcgg gaccccgtca gagggacgct gtcgaagcgc
      301 tgtgtcggga gatcgacacg gcccggctcc ggatccgtcc caactgcgcc gcccggacca
      361 tggtggtcga catccagccg atcgccgatg tgagccagaa gaccgggctg cccgtggagg
      421 cctgcatctt catcggcagc agcccgatcc gtcagtacgc ggaggagtgg gacctggacc
      481 acatcctccg gcacacgcgg caggcgacgg agttcgcggt gcgtgagggg ctggacgtga
      541 tgtacgtgac cgaggacacg gtccgctctt cgcccgagca cctgcgggtg ctcctgaccg
      601 ccgcgatcga cgcgggcgcc aggcgcgtct gcctgtgcga caccgtgggg gcggccgtcc
      661 ccgagggggt gatcaacctg acgcgctggg tccgggcgct gctggaggac ctcggcgtgg
      721 gcgagcgggt ggggaatacg ctcatggagc agctgctggt caacctccgg ctcctgggct
      781 ggcgggaggg cgacctcggc cgcctcaccg aatacaccga gctggtctcc caggcggtgg
      841 gcgtaccgat ccccgtgaat caaccgatcg tggggcgcga cgcgttccgc accgccacgg
      901 gcgtccacgc cgccgcggtc atcaaggcgc agcggaaggg gcaggcctgg ctggccgacc
      961 gcgtctattc cggcgtgccg gccgggtgga tcgggcggca ccaggaaatc gaggtcggcc
     1021 acatgtccgg gggctcgaac gtgcagcatt tcctggccgc tcgcggtctc acggccacgc
     1081 ccgagacggt ggaggcggtc ctggccgcgg ccaagcgctc gaagcgcctg ctcacggagg
     1141 aggaggtcct ccagcacgtg cgcaccccgg ccagttcctg aaagaagcgg atcgcggttc
     1201 gatccttcac atcggggtgg gccgactccg gtgttcgcgc ccgggaccgg gggatcacct
     1261 catcccgcgg ccccggccgc gcggccccat catcccggtg cctcttcccg agtccggccc
     1321 catcatgccc gggcccatgc cgccgtccgg ccgcatcatg ccgggaccca tccgcccccc
     1381 gggggtcatc gggggtcccg ctccggagtc cggccccatc atcccccgtc ccatgtactg
     1441 catcatccgc gcgtggtgcg cttgcatcca ctggcggaac ggagcgcggc gatcgggcgg
     1501 gagcgcgtcc tcgagccgct gccggccccg ccgtgccacg ctgcgcaggg aatcgggccc
     1561 cgcggtgacg gcccccgtca tctccacctg caggctgtcc agcaccgcat ccacgcgggc
     1621 gcgctgcgtg gaatccaggt ccagcgcccg gaccagccct tcgggggtga ggctcgcggc
     1681 ctccgccctc gggggccaga gaccgagcca gacccgatcg gccgccatac ctgccgccgc
     1741 tccgaccagg aagatgacgg ccgtggcgag cgcggccttc cgcgacgggt tcatgccgtt
     1801 cgatctcatc gcccccctcc ttcccgtgat gcgggctcga gggccaggag ggccaggagc
     1861 gggtctgcct caatgccggc acgttgccac agcgccgcgg gctcgccgcc gctggcgagc
     1921 gccgacagca ccagggccgc ggggtccggc tccgccccgg gcggaccggc cggcgcccgc
     1981 cccgatccca ggccgaccac cagtacgatg gacgcggcga gtcccatggc gcccgcgacc
     2041 gccggtcgcc accacaggct cagggcctgc caccacgaga cggccgcgga ggcgggcgct
     2101 tcgctcggac ggcgatagcg ttcgagcacc gggtccgcct cctccatcag ccgctcgagc
     2161 cgaagccgcc agtacgcgac atcctcctcg gaaggcaccg gggagtccgg gagccattcc
     2221 ggcggcagcg gttcagtgga gggtcctctc gtcgtcatgg tcggttcctg tcgttcagta
     2281 cctcgctgag gcgcgcccgc aggtcacgac gcgcgtggtg gagatgcgca cgg
//
LOCUS       DASQQQ010000307         3033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_97236, whole genome shotgun sequence.
ACCESSION   DASQQQ010000307 DASQQQ010000000
VERSION     DASQQQ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97236"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2538)
                     /locus_tag="VE646_11575"
     CDS             complement(<1..2538)
                     /locus_tag="VE646_11575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD80662.1"
                     /translation="MGSNHRRRFLRLSWRSPERIRADVDEELAFDLDMRAKELTAQGL
                     EPAEARRVALEEFGDLEATRRACAASDRVSQRSTRRAEWLGEVRQDVRLALRGAVRAP
                     GFTLVVLATLALGIGANTAVFSVMRKVLLERLPYREPGRLVRLYGGSVTNPGARSMLT
                     AAEISELQGAPELEAVAPFGYYGGYTWVGEERAEVWGGVRVGPGFFRMLGVSPLLGRG
                     IGQRDLEPEAPPVVVLGHALWQREFGGDPTVVGRAVRLNGREHVVVGVMPPEFVSPER
                     DPEIWTPLDLQALLRDHPAIARHGRMLRAVGRVRAGVDAARLRSALEAFAGRARADDP
                     ERGDAVAVRAVSLSSDMVGEVRPVLLIIMGAAALVLVLACANLAGLFLSRAASHRRHM
                     AVRAALGARRERLVRQLLTESTVLALAGGALGIPVAFWVRNGLVDVTASLLPSMRDAP
                     LDAAVLAFTAVLAVLTALAFGALPALTGTRLDLADSLAGTSRRVSGGAAASRTRHALV
                     GAQVGLAVLLSIAAGLLGRTLVALHRTGVGFDTGSAVLTFRVNLMSERYAGDEVRARF
                     FEGFAGRIRTLPGVRAIGTVDVSPWNGYTAFGPDSFAVVGRPSRVGGADLASRVTASV
                     GYFGALRIPVIQGRAFTSRDRAGSPLVALVNRSLAEEYWADASPVGAGIRVGGAAAPP
                     LQVVGVVGDVRPSPDSEVTPTVYVPVAQHPPGGAEFVVRAGGDARTLVPAIRRALHEE
                     DPALPLVAPRGLAEVLAAMLAGQRLPLLFMSAFAGLALLLASLGVYGVMAYSVTARRR
                     ELGVRIALGSSRGAVLALALKEAVTVALAGMAAGLLVALVGVRLLRGL"
     gene            complement(2556..2885)
                     /locus_tag="VE646_11580"
     CDS             complement(2556..2885)
                     /locus_tag="VE646_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZD80663.1"
                     /translation="MSARRGLLPGTVDVLILQTLQAEPMHGYGISRLVRERSGGLLTI
                     ESAALYQALHRLERKKWVRSEWGVSETNRRARFYQLTAKGREHLSREREELRSYMAAL
                     DLVLDPT"
BASE COUNT          494 a         1128 c         1092 g          319 t
ORIGIN      
        1 cagtccgcgg agcaagcgca ccccgaccaa cgcgacgaga agacccgcgg ccatccctgc
       61 gagagccacc gtcacggcct ccttcagcgc gagagcgagc acggcgcccc gggagctccc
      121 cagcgcgatc cgtacgccga gctcccgccg acgggcggtg accgagtacg ccatgactcc
      181 gtagacgccg agggaagcca gcaggagggc caggcccgcg aaggccgaca tgaagagcag
      241 gggcaggcgc tggccggcga gcatggcggc caggacctcg gcgagacctc ggggagccac
      301 gaggggcagg gccggatcct cttcgtgaag cgcccgccgg atggccggca ccagggtccg
      361 ggcgtcgccg ccggcgcgga ccacgaactc ggcgccgccc gggggatgct gggccacggg
      421 cacgtagacc gtcggcgtca cctccgagtc cggcgatgga cggacgtccc cgaccacgcc
      481 gacgacctgc agggggggtg cggccgcgcc acccacccgg atccccgccc ccaccgggct
      541 cgcatccgcc cagtactcct ccgccaggct ccgattcacg agggcgacca gcggcgagcc
      601 ggcgcgatcc cgggaggtga aggcccgacc ctggatcacc gggatgcgga gagcgccgaa
      661 gtacccaacg gaagccgtga cccgactggc gagatccgcg ccgccgaccc gggatgggcg
      721 ccccaccacg gcaaacgagt cgggcccgaa ggccgtgtag ccgttccagg gcgagacgtc
      781 gaccgtgccg atggcccgga cgccggggag ggtgcggatg cgccccgcga acccctcgaa
      841 gaagcgcgcc cgaacctcgt cgccggcgta ccgctccgac atgaggttga cgcggaacgt
      901 caggacggcg gaaccggtgt cgaacccgac tccggtccgg tgcagcgcca cgagcgtccg
      961 tcccagcagc ccggcggcga tggacagcag caccgcaagg ccgacctggg cgcccaccag
     1021 ggcgtgccgt gtacggctgg ccgccgctcc gcccgacacc cgccggctcg ttccggccag
     1081 ggaatccgcc aggtcgaggc gggtgccggt cagggccggc agagccccga aggcgagcgc
     1141 ggtgagcacg gcgagcaccg ccgtgaacgc gagcaccgcc gcgtcgagag gcgcatcacg
     1201 catcgacggc agcagcgagg ccgtgacgtc gaccagcccg ttccggaccc agaaggccac
     1261 gggaataccg agggctccgc cggccagggc caggaccgta ctctcggtga ggagctggcg
     1321 gaccagacgc tcgcggcggg ctcccagggc cgcccggacg gccatgtggc gcctgtggct
     1381 cgccgcccgg gagaggaaga gcccggcgag gttggcgcag gccaggacga ggacgagcgc
     1441 cgcggcgccc atgatgatca acaggacggg ccggacctcg ccgaccatgt ccgacgagag
     1501 ggacaccgcc cggacggcca ccgcatcgcc ccgctcgggg tcgtccgccc gggcccgccc
     1561 tgcgaaggcc tcgagagcgc tccgtagccg ggcggcgtcg acgccggcgc gcacccggcc
     1621 gacggctcgg agcatgcggc cgtggcgggc gatggcggga tggtcacgga ggagcgcctg
     1681 gaggtccagc ggggtccaga tctccggatc gcgctccggc gagacgaact ccggcggcat
     1741 cacgcccacc acgacgtgct cgcgcccgtt gagccggacg gcgcggccga ccaccgtcgg
     1801 atcccccccg aactcgcgct gccagagggc atggcccagc accacgaccg gcggggcctc
     1861 aggctccagg tcccgctgtc cgatcccccg gccgaggagg ggcgacacgc cgagcatccg
     1921 gaagaatccc ggacccaccc gcactccgcc ccacacctcg gccctctcct cgccgaccca
     1981 ggtgtagccc ccgtagtagc cgaagggggc gacggcctcg agctcggggg cgccctgcag
     2041 ctccgagatc tcggccgccg tgagcatcga acgcgccccg gggttggtca cggacccgcc
     2101 gtacaggcgc accaggcgtc ccggctcccg gtaggggagc cgctcgagca ggaccttgcg
     2161 catgaccgag aacacggcgg tgttcgcccc gatgcccagg gccaacgtgg ccagcacgac
     2221 gagggtgaag cccggcgccc ggaccgcgcc gcgcagggcc aggcgcacgt cctggcgcac
     2281 ctcacccagc cactcggcac gacgagtcga ccgctgggac acacggtccg acgccgcgca
     2341 tgcccggcgc gtcgcctcca ggtcaccgaa ctcttcgagg gccacgcggc gcgcttcggc
     2401 gggctccagc ccctgcgccg tcagctcctt cgcacgcatg tcgagatcga aggccagctc
     2461 ctcgtccacg tccgcccgga tgcgctccgg ggagcgccag gagagtcgaa ggaagcggcg
     2521 acggtggttg gagcccacgg acgtgtcccg gggaatcagg tcgggtcgag caccagatcg
     2581 agcgcggcca tgtacgaacg cagctcctcg cgctcacggc tcaggtgctc gcggcccttc
     2641 gcggtcagct ggtagaacct cgcccggcgg ttggtctccg agacccccca ctcggaccgg
     2701 acccacttct tccgctcgag ccggtggagg gcctggtaga gggccgcgct ctcgatggtc
     2761 agcaggccgc cggagcgctc ccgcacgagg cgcgagatgc cgtagccgtg catgggctca
     2821 gcctgcagcg tctgcaggat gagcacgtcg acggtgcccg gcaggagccc gcgtcgggcc
     2881 gacatcaccg ttctccagtc acatgattat aggtaatcag atgacctgag aatgcccggc
     2941 agcacggggg gtgtcaagag ggcccctcga gatcgtcccc gttctctccc ggttcccttc
     3001 aggggcggag ggggaagaag gcgagatcgg agc
//
LOCUS       DASQQQ010000308         4456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_20906, whole genome shotgun sequence.
ACCESSION   DASQQQ010000308 DASQQQ010000000
VERSION     DASQQQ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20906"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /locus_tag="VE646_11585"
     CDS             complement(<1..1425)
                     /locus_tag="VE646_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80664.1"
                     /translation="MRTEEPHTVVAGRYELAEELGRSAAGASWRATDAVLHRSVVVEL
                     VHPDLVHHPSFAERFGRSTRAVASIEHPGLARLLDAGSDQGVFFVVREDVEGRTLRAI
                     LDTQGPLPLPRAIQVTRAVLEALAPIHRLGIVPLALGPEDVVVEPSGRVRVCSAAAGA
                     VAEAGPPLGEVEAQAADGAGRDAADPRPPTVADPRADVKRAAILLFELITGSPPDRTR
                     ARARRPAIPRALDPVLRRALSADPDARYASAEAFAAALAQVGDGMSMPGRPMAPGAAS
                     ARRPSLFRTWLAVPLLVAVLAVGAGVAGLSFGRLELGGPVGIRLREESPSPSPVPRVL
                     SFASDPQTFDPFGDGQENDAGAPLATDGDPSTAWKSENYFDGQLHKPGVGLLFDLGSA
                     RTVTGFRLSTPFPGFDFKVSVGSDPSAMAERARGTTYTARDDAPVMLDRPQRGRYVLL
                     WITSVVPVDDGNRAEVAEFRVLGVP"
     gene            complement(1466..1867)
                     /locus_tag="VE646_11590"
     CDS             complement(1466..1867)
                     /locus_tag="VE646_11590"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HZD80665.1"
                     /translation="MRGPRFSGQRGLVGKIVVVWLVFLVLFGVAAWDAGTVMIARFKL
                     QNAAENAAFEAASAFKTTRDLRQAKQAALDQLKQDADGARMTKFRVDQTTGKVTVSLS
                     ERVSTVLAGRIGYFKKLTRATASHTSGPPTL"
     gene            complement(1864..3525)
                     /gene="murJ"
                     /locus_tag="VE646_11595"
     CDS             complement(1864..3525)
                     /gene="murJ"
                     /locus_tag="VE646_11595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HZD80666.1"
                     /translation="MNPEPSGTAELQPDDSQRAFVRRTAVMSVATSLSRITGFLRVSA
                     TAFALGVAGTRLATAYTVANNTPNIVYELILGGILTSVFVPVFVEWLQDRGREEAWDV
                     ARSVLSIAFVILAAVTLLTVLGAPWIIRLYTVGDSGPGAQAARDFATFCLRLFMPQIV
                     FYGIGAVITGLLNAHRRFAVPMFAPILNNLIVIGTMAAFVAFSSLPSGVEPRPENITS
                     LQRWILALGTTLGVVGMTVALWPSLRRVGFRFRWRLEWGHPAVRRVARLALWTFVYVV
                     VNQLGLLVVIVLATGSNGYFAYTSAFILFQLPHAIFAVSIFTALLPAMATRWTNGDRE
                     GFRDQLSQGIRATAVILIPAALGMLVLATPLVRLVIQHGAANQQDTRLVSGILVFFAL
                     GLFSFSLFQLLLRAFYAMQDTRTPALVNIAAVGLNTAVNFVYFFSLHLGVRGLALGHA
                     TAYTFGSVVMLILIRGRLGGLDGRRVLSTLARTLVAGAGTALVAWAAAEGLARALGTA
                     SVATQVLQVFGATGAGLLAFLLAALMLRIDEVDAVRRQLIARWRA"
     gene            complement(3522..>4456)
                     /locus_tag="VE646_11600"
     CDS             complement(3522..>4456)
                     /locus_tag="VE646_11600"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HZD80667.1"
                     /translation="PAVAEVEARDRTMTAVVPDLGLQTLLGSDLVRDDPVRGAQAVLG
                     ELAQIWLEQPGTERGVALSFGEGLQAPGAFFGPLIRRVSQAPWLRKLPASTLADRFPP
                     RSPTPLATLGGASFGRSYVEDIRDARRLIDTYRSVLVQNGTEPERLEDRLLMAESGRF
                     LADQGQGLEFVDAVRNRVEGVFDKIRADPQQPNTLTSSSGKVFVPVTNGNDMPVRVTV
                     RLVSNHLQRTPVSTTVLPAETTQTLGFEVHLNTTGRFPVDIQISAPSGRLIAQGTLIV
                     RSTAYNRIALLITIGAAFLALMLWARRFLPRRTS"
BASE COUNT          732 a         1585 c         1575 g          564 t
ORIGIN      
        1 cggcaccccc agcacccgga actcggccac ctcggcccgg ttcccgtcgt ccacgggcac
       61 gacggaggtg atccacagca ggacgtaccg cccgcgctgg gggcgatcga gcatcaccgg
      121 agcgtcgtcg cgagccgtgt aggtggttcc gcgagcccgc tcggccatcg cgctcgggtc
      181 gctgcccacc gataccttga agtcgaaccc cgggaacggc gtgctcagac ggaacccggt
      241 cacggtccgg gccgagccga gatcgaagag gaggcccacg cccggcttgt ggagctgccc
      301 gtcgaagtag ttctcggact tccaggcggt ggaagggtcg ccgtccgtcg cgagcggtgc
      361 tcccgcgtcg ttctcctgcc cgtccccgaa cgggtcgaag gtctgggggt ccgaggcgaa
      421 ggacagaacg cggggcacgg gggagggcga tggcgactcc tcccggagcc ggatcccgac
      481 cggcccgccc agctccaggc gtccgaagga cagcccggca accccggcac ccaccgccag
      541 caccgccacc aacaggggca ccgccagcca ggtccggaac agcgacggac ggcgggcgct
      601 cgccgcaccc ggggccatgg ggcgcccggg catgctcatc ccatccccca cctgcgcgag
      661 ggcggcggcg aaagcttcgg ccgaggcgta ccgggcatcg gggtcggcgc tcagcgcccg
      721 acggaggacg gggtcgaggg ctcgagggat agccggccga cgcgcccgcg ctcgggttcg
      781 atccggcggc gatcctgtga tcagttcgaa cagcaggatg gccgcccgct tcacatcggc
      841 tcgagggtcg gccacggtgg ggggccgggg atcggccgcg tcccggccgg ctccatcggc
      901 tgcctgggct tcgacctcgc cgaggggtgg cccggcttcg gcgaccgcgc ccgcggccgc
      961 cgagcacacc cgcacgcgac cgctcggctc gaccaccacg tcctccggcc ccagggccag
     1021 cggcacgatg cccagtcggt ggatcggagc gagcgcctcc agcacggctc gcgtcacctg
     1081 gatcgccctg gggaggggaa gaggcccctg cgtgtctagg atcgcgcgca gggtgcgccc
     1141 ctcgacgtcc tcacggacca cgaagaagac cccctggtcg ctgccggcat ccaacagcct
     1201 ggccagaccg ggatgctcga tcgaggccac cgcgcgggtg gagcggccga agcgctcggc
     1261 gaaggagggg tggtgcacca ggtcggggtg caccagctcg accaccaccg agcgatgcag
     1321 gaccgcgtcg gtggcacgcc acgaggcacc cgcggcgctc cgccccagct cctcggccag
     1381 ctcgtagcgc ccggccacga cggtgtgggg ttcctcggtc ctcacccgcg aagtctacgt
     1441 ccctgcctgc ggccggagcc gctggtcata gggtgggtgg gcccgacgtg tgcgaggcgg
     1501 tcgccctggt cagcttcttg aagtagccga tccgtccggc caggaccgtc gacacccgtt
     1561 ccgacagcga gacggtgacc ttgcccgtgg tctggtccac cctgaacttc gtcatcctcg
     1621 cgccgtccgc gtcctgcttc agctggtcca gcgcggcctg cttggcctgg cggaggtcac
     1681 gggtggtctt gaacgcggag gccgcctcga aggcggcgtt ctccgccgcg ttctggagct
     1741 tgaaccgcgc gatcatcacc gtccccgcgt cccacgccgc gacgccgaac aggaccagga
     1801 acacgagcca gaccacgacg atcttgccca ccaggccccg ttgtcccgag aacctcggcc
     1861 ccctcatgcc ctccacctgg cgatcagttg tcgacgtacg gcatcgacct cgtcgatccg
     1921 caacataagg gccgcgagca ggaacgcaag cagcccggcc cccgtggcgc cgaacacctg
     1981 caggacctga gtggccacgc tcgccgtgcc gagggccctg gcgaggccct cggccgcggc
     2041 ccaggcgacc agcgcggtgc ctgcacccgc caccagcgtg cgagccagcg tggaaaggac
     2101 ccgacgtccg tccagaccgc cgagccgacc gcggatgagg atcagcatca ccaccgagcc
     2161 gaacgtgtag gcggtggcgt gtccgagggc gagccctcgc acccccaggt gcagggagaa
     2221 gaagtacacg aagttgaccg ccgtgttcag acccacggcc gcgatgttca ccagggccgg
     2281 ggtgcgcgtg tcctgcatcg cgtagaaggc gcggagcagc agctggaaca gcgagaagga
     2341 gaacaacccc agggcgaaga acaccaggat gcccgagacc aaccgggtgt cctgctggtt
     2401 cgcggccccg tgctggatca ccaggcggac caagggggtg gccagcacca gcatccccag
     2461 ggcggcgggg atcaggatga ccgcggtggc ccggatcccc tgcgagagct gatcgcggaa
     2521 cccctcgcgg tcgccgttcg tccagcgtgt ggccatggcg ggcagaagcg ccgtgaagat
     2581 ggacacggcg aagatcgcgt ggggcagctg gaacaggatg aacgcggacg tgtacgcgaa
     2641 gtagccgttg ctgcccgtgg ccagcacgat caccaccagg agccccagct ggttcaccac
     2701 cacgtacacg aacgtccaca gcgccagtcg ggccacgcgg cggacggcgg ggtggcccca
     2761 ctccagccgc caccggaacc ggaatcccac ccgtcgcagc gacggccaca gggccacggt
     2821 catccccacc accccgagcg tggtgccgag cgccaggatc cagcgctgca gggaggtgat
     2881 gttctcgggg cggggctcca cgccggaggg caaggacgag aaggccacga acgcggccat
     2941 cgtcccgatc acgatcaggt tgttgaggat cggggcgaac atcggcacgg cgaaccggcg
     3001 gtgggcgttc agcagcccgg tgatcacggc cccgatcccg tagaagacga tctggggcat
     3061 gaacagccgg aggcagaacg tggcgaagtc tcgggccgcc tgcgctcccg ggcccgagtc
     3121 gcccacggtg tagaggcgga tgatccacgg ggcgcccagc accgtgagca gggtgaccgc
     3181 ggccaggatc acgaacgcga tcgacaggac cgagcgggcg acgtcccagg cctcctcccg
     3241 tccccggtcc tggagccact ccacgaacac cgggacgaag accgaggtca ggatgccccc
     3301 caggatcagc tcgtagacga tgttcggggt gttgttcgcc accgtgtagg cggtggccag
     3361 ccgggtcccg gccaccccga gggcgaaggc ggtggccgag acccgcagga acccggtgat
     3421 ccgggagagc gacgtcgcca ccgacatcac cgcggtccgc cgcacgaagg cccgctggga
     3481 gtcgtccggc tgcagctctg ccgtcccgga cggttcggga ttcacgacgt ccgacgtggg
     3541 aggaacctcc gggcccacag catcagcgcc aggaaggcgg cgccgatggt gatcaggagc
     3601 gcgatccggt tgtacgcggt ggaacggacg atcagcgtgc cctgagcgat gagccgtccc
     3661 gacggggccg agatctggat gtcgaccggg aaccggccgg tggtgttgag gtgcacctcg
     3721 aagcccaggg tctgcgtggt ctcggccggc agcaccgtgg tagagaccgg cgtccgctgc
     3781 aggtggttcg agaccaggcg cacggtcacg cgcaccggca tgtcgttgcc gttcgtgacc
     3841 ggcacgaaga ccttcccgct gctggaggtg agggtgttcg gctgctgcgg atcggcccgg
     3901 atcttgtcga acaccccctc cacccggttg cgcaccgcgt ccacgaactc caggccctgt
     3961 ccctggtcgg ccaggaaccg ccccgactcg gccatcagca atcggtcctc gagccgctcc
     4021 ggctccgtcc cgttctgcac cagcaccgag cggtacgtgt cgatcagccg gcgggcatcg
     4081 cggatgtcct ccacgtagct ccggccgaat gaggcgccgc cgagggtggc gaggggggtg
     4141 ggggaacggg gcgggaagcg atcggccaac gtggaggcgg ggagcttgcg caaccatggc
     4201 gcctgggaga ccctccggat caggggaccg aagaacgccc ccggcgcctg caggccctca
     4261 ccgaaggaca aggccacccc ccgttccgtg cccggctgct cgagccagat ctgtgcgagc
     4321 tcgcccagca cggcttgcgc tccgcggacc gggtcgtccc tcacgaggtc cgatcccagg
     4381 agggtctgga ggccgagatc cgggaccacc gcggtcatgg tcctatcccg ggcttccacc
     4441 tccgccaccg ccggag
//
LOCUS       DASQQQ010000309         3955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_299185, whole genome shotgun sequence.
ACCESSION   DASQQQ010000309 DASQQQ010000000
VERSION     DASQQQ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299185"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2077
                     /locus_tag="VE646_11605"
     CDS             <1..2077
                     /locus_tag="VE646_11605"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HZD80668.1"
                     /translation="KLKEAYLAYQLEQQMSKDEILTRYLNTVYFGNGAYGIQAAAETY
                     FDRPAGRLNLNQAALLAGLIQAPNDFDPVAHPRAARQRRTEVLANMRDLGMITDAAYQ
                     RASAHPVNLRLQLGRQQHYLAPYFVDYFKQWFLSNPRFGKTVEDRYNLLFEGGLRIQT
                     TLVPRLQRQAETAVRSILTYRRDPYGAMTVLDPRTGYIKAMVGGRDYWSPSDRYARIN
                     LAAGGTTGRQAGSAFKPFALVAALEDGLPPSTRLNGSSARIPLQNGTYWEPGNAEGGG
                     YGTISLETATVNSVNIAYANLLEKMGGGDAYVGARQMVQVAKRMGIRCCTRTTSPRTP
                     LQAVPSAVLGTNEVNTVEMASAYGTLAYGGQHVQPTPVIRIVAPDGKVLYQANPKPRR
                     VVPPAVITVADGILQKVVLYGTGAAANLGRPQIGKTGTAQNYSDAWFVGAIPQLVAAV
                     WVGFPQGQISMAYPRVRISRVYGGTWPAQIWRAFMVNATKHIPAKAFPSSNVTYVTVR
                     VDVTQGCLANPYTPPANIQTIQYISGTEPQRICKEPRSYQFLAVPSVIGLKENQALAT
                     LQAAGFNITSQYRGSDQPVGTVIAQDPAAGQQAQQTSTVTITVAKNPAPSPSPQQAIV
                     PDVIGMSRSDAVAALQDAGFAVTVVKDHQCSPSDPGCHEQKGVVWSQDPSGGEQAREG
                     TTVTIVVSP"
     gene            complement(2055..2744)
                     /locus_tag="VE646_11610"
     CDS             complement(2055..2744)
                     /locus_tag="VE646_11610"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019329393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZD80669.1"
                     /translation="MAVGQAAPLPPEGSTRPVVTAPTRRLARAFYRRPSLEVAPDLLG
                     RHLVRRLPDGRVLTVRIVETEAYREDDPASHSFRGRTPRTVVMFGPPGRLYVYFTYGM
                     HFCMNVVTGPDGEGSAVLLRAAEPLSGIDEMARRRGLEAPGLLCSGPARLCQAVGVDR
                     SLNGTDLVRGGDVWLVEGTPVPPSLVGRGPRVGIRVGVERRWRFFVLDDPYVSRPRPL
                     GPARVRGSRRS"
     gene            complement(2687..>3955)
                     /locus_tag="VE646_11615"
     CDS             complement(2687..>3955)
                     /locus_tag="VE646_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018384516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZD80670.1"
                     /translation="RIQVNVALDVASDLLGELSQLEEVERATYAGSLRRMAETIGDVD
                     LLVASAHPRPIMERFVGLPLVDRVLASGETKSSILTTGGLQVDLRVIPADTWGAALIY
                     FTGSKAHNIRLREMAIRKGLRLNEYGLFRVDTGKRIVARTEDEVYGRLGLPPISPTLR
                     EDRGEIEAALAGELPAVLEQRQIRGDLHTHTDLTDGLATLEEMLEAAAGYGYAYYAVT
                     DHAPNLAMQRMTDEKILAQRQRLRELQSRYPRMTLLHGSELNIDPDGGVDWPEDFLEG
                     FDVLVASVHSHFSQSKDEMTRRIVRAMENPRVHVIGHPTARVIGRRQPIEFDFEEVFK
                     AAARTGTAMEVNSFPDRLDLKDEHILWARRHGVKFAVDTDSHATVHLGAMRFGVATAQ
                     RGWLTKDDVINAWPLAKLRRFLQKGRRARS"
BASE COUNT          644 a         1430 c         1326 g          555 t
ORIGIN      
        1 gaagctcaag gaggcctacc tggcctacca gctggaacag cagatgtcca aggacgagat
       61 cctcaccagg tacctgaaca ccgtctactt cggcaacggc gcctacggga tccaggccgc
      121 cgccgagacc tacttcgacc gtcccgccgg ccggctgaac ctgaaccaag ctgccctgct
      181 ggccgggttg atccaggcac ccaacgactt cgacccggtg gcgcacccca gggcggcccg
      241 gcaacgccgg accgaggtgc tggccaacat gcgggacctg ggcatgatca ccgacgcggc
      301 ctatcagcgg gcgtcggccc acccggtgaa cctgcgtctg cagctcggtc ggcaacagca
      361 ctacctggcc ccctatttcg tggactactt caagcagtgg ttcctctcga acccccgctt
      421 cggcaagacc gtcgaggatc gctacaacct gctcttcgag ggaggactgc ggatccagac
      481 caccctggtc cctcgcctgc agcggcaggc ggagaccgcc gtccgctcga tcctcaccta
      541 caggcgcgac ccgtacggcg cgatgaccgt gctcgacccc cggaccggct acatcaaggc
      601 catggtcggt ggtcgtgact actggtcccc ctccgaccgc tacgcccgga tcaacctggc
      661 agccgggggc accaccggcc gccaggcggg gtcggcgttc aagccgttcg ctctggtggc
      721 cgccctcgag gacggccttc ccccgagcac ccgcctgaac gggtcctcgg cccggatccc
      781 gctgcagaac ggcacctact gggagcccgg gaacgcggag ggcggcggct acgggaccat
      841 ctccttggag acggccaccg tgaactcggt caacatcgcg tacgccaacc tgctggagaa
      901 gatgggcggg ggcgatgcct acgtcggggc ccgccagatg gtgcaggtgg ccaagcggat
      961 ggggatccgc tgctgcaccc gaaccacctc ccccaggacc cccctccagg ccgtcccctc
     1021 cgccgtgctg gggacgaacg aggtgaacac cgtggagatg gcctcagcct acgggaccct
     1081 ggcctacggc ggtcagcacg tgcagccgac acccgtgatc cgaatcgtcg cacccgacgg
     1141 caaggtcctg taccaggcca accccaagcc gaggcgggtg gtgcccccgg ccgtgatcac
     1201 cgtggcggac ggcatcctgc agaaggtggt cctctacggg accggagcgg cggcgaacct
     1261 cgggcggccc cagatcggca agaccggaac ggcgcagaac tacagcgacg catggttcgt
     1321 gggcgcgatc ccgcagctcg tggccgcggt ctgggtgggg ttcccgcagg ggcagatctc
     1381 catggcctat ccgagggtgc ggatctctcg cgtctacgga gggacctggc cggcgcagat
     1441 ctggcgcgcg ttcatggtca atgccaccaa gcacatcccg gccaaggcct tcccctcgtc
     1501 caacgtcacc tacgtgacgg ttcgggtgga cgtcacccag gggtgcctgg ccaacccgta
     1561 tacgccgccg gccaacatcc agacgatcca gtacatctcg gggaccgagc cccagaggat
     1621 ctgcaaggag ccccggtcct accagttcct ggcggtaccc tccgtgatcg ggctcaagga
     1681 gaaccaggcc ctggctaccc tccaggctgc cgggttcaac atcacctctc agtaccgagg
     1741 ctcggaccag ccggtgggca cggtcatcgc ccaggacccg gccgccgggc agcaggccca
     1801 gcagaccagc acggtgacca tcacggtggc gaagaacccc gccccttcgc cctcgccgca
     1861 gcaggcgatc gtccccgacg tgatcgggat gagccgctcg gatgcggtgg ccgccctgca
     1921 ggacgcgggg ttcgcggtca ccgtggtcaa ggaccaccag tgcagcccct ccgatccggg
     1981 ctgccacgag cagaagggcg tggtgtggtc ccaagacccc agcggcggcg agcaggccag
     2041 ggagggcacc accgtcacga tcgtcgtgag cccctgaccc gcgcaggacc gagggggcgc
     2101 ggccgggaca cgtacgggtc gtccaggacg aagaaccgcc atcggcgctc gacccccacc
     2161 cggatcccca cgcgcggacc tcgtcccacc agagagggcg gcaccggcgt gccctccacc
     2221 agccacacgt ccccaccccg gaccaggtcg gtgccgttca gggaccggtc cacgcccacg
     2281 gcctggcaga gccgcgccgg gccggagcag agcaggccgg gcgcctccag gccgcggcgt
     2341 cgggccatct cgtcgatccc cgagagaggc tcggccgccc ggagcaggac ggcactgccc
     2401 tcaccatcgg gcccggtgac cacgttcatg cagaagtgca tgccgtaggt gaagtagacg
     2461 tacagccggc cgggcgggcc gaacatcacc acggtccgag gggtgcgacc gcggaaggag
     2521 tgactggccg ggtcgtcctc gcggtacgcc tcggtctcca cgatgcgcac cgtgagcacg
     2581 cgaccgtcgg ggagtcggcg caccaggtgc cgacccagca ggtccggggc cacctccagg
     2641 gagggccggc ggtagaaggc tcgcgcgagc cggcgggtgg gagcggtcac gaccgggcgc
     2701 gtcgaccctt ctggaggaag cggcgcagct tggccaacgg ccatgcgttg atcacgtcgt
     2761 ccttcgtgag ccatccccgc tgcgccgtgg ccaccccgaa gcgcatcgct ccgagatgca
     2821 ccgtggcgtg cgagtcggtg tccacggcga acttcacgcc gtgacgcctg gcccacagga
     2881 tgtgctcgtc cttcaggtcc agccggtcgg gaaaggagtt gacctccatc gccgtgccgg
     2941 tccgggcggc cgccttgaag acctcctcga agtcgaactc gatcggctgg cgacgcccga
     3001 tgacgcgggc cgtggggtgg ccgatcacgt gcacccgggg gttctccatg gcccgcacga
     3061 tccggcgcgt catctcgtcc ttggactggc tgaagtgcga gtggaccgag gccaccagca
     3121 cgtcgaaccc ctccaggaag tcctccggcc agtccacccc tccgtcggga tcgatgttca
     3181 gctcggagcc gtgcagcagg gtcatcctcg ggtagcggga ctgcagctcg cgcaggcgct
     3241 ggcgctgggc caggatcttc tcgtcggtca tccgctgcat ggccaggttc ggcgcgtggt
     3301 cggtgacggc gtagtacgcg tagccgtagc cggccgccgc ctcgagcatc tcctccaggg
     3361 tggccaggcc gtcggtcagg tccgtgtggg tgtgcaggtc accgcggatc tgccgctgct
     3421 cgaggacggc ggggagctca ccggccagcg ccgcctcgat ctcaccgcga tcctcccgca
     3481 gggtggggga gatcgggggc agccccaggc gcccgtagac ctcgtcctcc gtgcgcgcca
     3541 cgatccgctt gcccgtgtcg acccggaaga gcccgtactc gttcaggcgg agtcccttgc
     3601 ggatcgccat ctcgcgcaga cggatgttgt gggccttgga gcccgtgaag tagatcaggg
     3661 ccgctcccca cgtgtcggcg gggatcaccc gcagatccac ctggagccca cccgtggtga
     3721 ggatcgagga cttcgtctcc ccgcttgcca gcacccggtc gaccagcggc agacccacga
     3781 accgctccat gatgggccga gggtgggcgc tggccaccaa caggtcgacg tcgccgatcg
     3841 tctcggccat ccggcgcagg gagccggcgt aggtggcccg ctccacctct tcgagctgcg
     3901 agagctcgcc cagcagatcg ctcgccacgt ccagggcgac gttcacctgg atccg
//
LOCUS       DASQQQ010000310         9306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_49884, whole genome shotgun sequence.
ACCESSION   DASQQQ010000310 DASQQQ010000000
VERSION     DASQQQ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9306
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49884"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VE646_11620"
     CDS             complement(<1..439)
                     /locus_tag="VE646_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZD80671.1"
                     /translation="MDGQRLFAEAISQALTRLGADPICVRTTPEEATATAADHQPDLI
                     LVEIAIGGVDGIRLGGELGEVSPSSKVVALTSVARPVVVRRAMAAGFHGFLTKDTSAE
                     GFQRSVRAILDGRLVFPGRPRKVVAHPGGASANGHGDGHGNGSG"
     gene            complement(661..2277)
                     /gene="groL"
                     /locus_tag="VE646_11625"
     CDS             complement(661..2277)
                     /gene="groL"
                     /locus_tag="VE646_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014674420.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZD80672.1"
                     /translation="MASKELKFSEEARRSLEAGVNKLADTVALTLGPKGHNVVLDKKW
                     GAPTITNDGVTIAKEIELEDPWENMGAQLAKEVATKTNDVAGDGTTTATVLARAMVRH
                     GMRNVAAGANPMALKRGIEKAVAAAVEVIKEQAKDIESRDEIAQVAAISAADPQIGEV
                     LAEALDKVGKDGVVTVEESNTFGMELEFVEGMQFDKGYISPYFVTDQERMEAVLDEPY
                     IVLVNKKVGSVQELLPLLEKVLQAGKPLLLVAEDIEGEALATLVVNKIRGTFNAAAVK
                     APGFGERRKAMLQDMAILTGGQVISEEVGLKLENVGLDLLGKARRVVVTKDDTTIVEG
                     AGGEDEVRGRITQIKAEIDKTDSDWDREKLQERLAKLAGGVAIVKVGAATEVELKEKK
                     HRIEDALSATRAAIEEGIVPGGGVALIRAEQTLGKLDLAGDEATGATIVRDTLAAPAR
                     LIAENAGYEGAVVVDRIRTEGDGRGFDAATGEWVDMVKTGIIDPAKVTRSALQNAASI
                     AALVLTTESAVVEKPEEEEPAAPAGHGHMH"
     gene            complement(2351..2650)
                     /gene="groES"
                     /locus_tag="VE646_11630"
     CDS             complement(2351..2650)
                     /gene="groES"
                     /locus_tag="VE646_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZD80673.1"
                     /translation="MAKEFSLKPLEDRIVVKPGEEEETTVSGIVIPDTAKEKPVEGEV
                     VAVGPGRFEDGNRVPLDVKVGDKVIYSKYGGTEVKVAGDDYLILSARDVLAVVQK"
     gene            2916..5072
                     /locus_tag="VE646_11635"
     CDS             2916..5072
                     /locus_tag="VE646_11635"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HZD80674.1"
                     /translation="MRKGRGHRRAITAVLAAGTLLTGSLTPSASPAAAAEPVGSLQAT
                     IDTSSWSPPSPDPTGITWWTDRLLVVDSEVEETISGITHYEGANTYEASPSGTLERTS
                     TTTAFSTEPTDVAAHGSRVFVSDDNRDRIFVIHVGPDGRLNTSDDTRTSIGTHAFGSY
                     DDEAVSFAGGDLFAGDGTNTQIYRIDPGPDGAFGGTDDRVTCFDTRALGMRDPEGVTV
                     DPATGRIYVASRISREIAVADGSGALLNLIDLRPILPYGGRDRIAPSGVTLAPAAEDP
                     SRIDLYLTDRGVDNNTDPSENDGAIYQIRRQSPDGNVAPVVSGFGALRAVEGERVSVR
                     TVAADPNGDPLSYSATGLPPGLSIDPSSGLITGKVAAGAAAGSPYVSHVTVSDGSLAT
                     VRRARWRITTGAPDDRAPVISPTGDQRSTEGDPVALRIQASDPDVGQALMYSATGLPP
                     GLWLDCTSGRIRGRVASGAASGSPYRVRVQVEDQTIPADPSFTSRFDQESFSWTVSAP
                     GPSPSITFRDASKGSTPGPSRRLRIPSPSGVAPGDVLVAAIDVRGPARIGSPRGWVRV
                     RADHRGHRLRQFVYVRVLTGDEPGTWRWRLSRRQPATGIIVGYRGVDPIDPIEVAAGR
                     VKRRSRWITAPSVTAAEPSAMVVGMFGVAARAWIRPPRGMDERAEIRLRRSRRRITTE
                     VSDRLLSDAGPTGRLVARATRAGTNIGQSLILRPAS"
     gene            5197..5919
                     /locus_tag="VE646_11640"
     CDS             5197..5919
                     /locus_tag="VE646_11640"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZD80675.1"
                     /translation="MLRGDVVAFGDVETEEIDPQGTAQAALEGDGIRLHGAGWRLVKR
                     LLDVVLSLTLILLTLPLMLLIALAITAESRGPLFFVHRRMGKGRVPFGLLKFRTMVPD
                     AEERLEAHLAEHPEHLAEWRQYYKLRDDPRITRVGRLLRKSSLDELPQLFNILRGDMS
                     VVGPRAIVRDEVPRFERYADTMLSVKPGLTGLWAVSGRNDVTQQHRAVLEYRYVTDWS
                     LLLDLWILFRTIPAVIRGHGAY"
     gene            complement(5998..7293)
                     /locus_tag="VE646_11645"
     CDS             complement(5998..7293)
                     /locus_tag="VE646_11645"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZD80676.1"
                     /translation="MAVPEAGSAGTPRDPGGASAPRKTLLLLCEHFYPEMISTGMHMT
                     ELATRLAELGWEITVYTAKPSWGTDEGDGRAVPKELVHEGVRILRVPTIGSQRGGLVS
                     KTVASVSFVLSAAWALLRQRSDHAGMIITTNPPFLGVLGWLVSRLRRRPYLIIVYDVF
                     PDFAVSLGVLRADSWLAKVWARTTRLILAGAAVTVVIGRDMLDLIKRKMPERTHDRIV
                     VIPNWSDERHVHPIPREENTFRAEHHLQDRFVVQYAGRLGEKHNLEPLIDAARLLEGE
                     PVLFQFVGEGAKKPKLQAMAAEHRLRNVQFLPYQPMHRLAEMLSAADLAVVCLEAGHT
                     GISVPSKAYGVIAGGTPLMGILDPDSEIGQMIKETGCGLLVEPEGREIARAIRGLVAE
                     PERREAMGEAGRAAFLEKYTLSAAARAYDAALTSMIAGR"
     gene            7532..8533
                     /locus_tag="VE646_11650"
     CDS             7532..8533
                     /locus_tag="VE646_11650"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZD80677.1"
                     /translation="MSQTGTPIKVLYVLGPGRSGTGILGRVLSTMEGAVFAGELRRLW
                     SRGLRPGRTCACGRPHAECPVWSRLLVPGAPFAHPSLQALGAIQARVAPEHLGWRAGL
                     RLRHLQAPPAPDTPEGRYLAAYTALHEAFARVTGAQVVIDSSKSAADASLLAVRREVP
                     TSIVQIVRDPRGVAFSLQQHYAKDASGLRRHLLALRGAVRWVAKHLTNEALRRRYGPD
                     RSIVLHYERLIEDPRSVVEEVAKTVGLAAPAIELSPGVPIEVPEVHGPDGSKRRRFVP
                     TQIVLRLDTRWQAELDPVDRFLVTAVTFPLLLRYGYPLRTPRPSASTTTRPGTEVEG"
     gene            8545..>9306
                     /locus_tag="VE646_11655"
     CDS             8545..>9306
                     /locus_tag="VE646_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZD80678.1"
                     /translation="MGVQRGTEPDPSRPENRTGSLDAGPSSKLPTFLLIGAMKAGTTS
                     LYHYLRGHPQVFMPEVKEVMFFDPRHNWHRGIEWYEAQFGGAEARHLARGEASTSYTK
                     FPVVQGVPARIASVLPDVRLLYVVRHPVERMQSHYLYLLSRGKESRPIERAFAEEPMY
                     LDTSRYALQLEQYEPYIRPEQLLLIDSRDLRDRRVETLGGIFAFLGVDATWVPPTVDR
                     EFFRSADRRMRPALSRAVRRLPGLSAAARRAPRPLR"
BASE COUNT         1252 a         3417 c         3217 g         1420 t
ORIGIN      
        1 taccggagcc gttgccgtgg ccgtcaccgt ggccgttggc ggaggcgccc cccggatgcg
       61 cgaccacctt cctgggccgt cccgggaaca ccagacggcc gtccaggatc gcccgcaccg
      121 agcgctggaa gccctccgcg ctcgtgtcct tcgtgaggaa gccgtggaac cccgccgcca
      181 tggcccgtct gaccaccacg ggacgggcca ccgaggtaag cgccaccacc ttggacgagg
      241 gcgacacctc cccgagctca ccgccgagcc ggatcccgtc cacgccgccg atcgcgatct
      301 ccaccaggat caggtccggc tggtgatcgg ccgcggtggc ggtcgcctcc tcgggggtgg
      361 tgcgaacgca gatcggatcg gccccgagcc gggtcagagc ttgactgatg gcctcggcga
      421 agagccgttg cccgtccacg atcagcgcgt ggggatcgat cgcgggcctc ctgttccggt
      481 ggggctgaac cctctcagtg tccgtaggcg cttcggggaa gtcaaacccc ctcgatacct
      541 acggcaaacg cagggtgcgt tcccgccatc ttcgaacacg gccctcgctg ggatgcgacg
      601 gggccgggga tcgctccccg gccccgtgtc cggttcgtgc cgtcgaacgg tcgtttccgg
      661 ctagtgcatg tgtccgtggc ccgccggggc cgcgggctcc tcctcctcgg gcttctccac
      721 caccgcgctc tcggtggtca gcaccagcgc cgcgatggag gccgcgttct gcagggcgga
      781 acgcgtgacc ttggccgggt cgatgatccc ggtcttgacc atgtcgaccc actcgccggt
      841 ggcggcgtcg aagccgcggc cgtccccctc ggtgcggatc cggtccacga ccaccgcacc
      901 ctcgtagccg gcgttctcgg cgatcagacg ggccggggcg gccagggtgt cacggacgat
      961 ggtcgcaccg gtggcctcgt cgccggccag gtcgagcttg ccgagcgtct gctccgcgcg
     1021 gatcagcgcc acgcccccgc cggggacgat cccttcctcg atcgcggccc gggtggccga
     1081 cagcgcgtcc tcgatgcggt gcttcttctc cttgagctcg acctcggtgg ccgcgcccac
     1141 cttgacgatg gccacaccgc cggccagctt ggccaggcgc tcctgcagct tctcccggtc
     1201 ccagtccgag tcggtcttgt cgatctcggc cttgatctgg gtgatccggc ccctgacctc
     1261 gtcctcgccg ccggcgccct ccacgatggt ggtgtcgtcc ttcgtcacca ccacgcggcg
     1321 ggccttgccc agcaggtcca gcccgacgtt ctccagcttc aggccgacct cctcggagat
     1381 cacctgcccg ccggtgagga tagccatgtc ctggagcatc gccttgcgcc gctcgccgaa
     1441 gccgggggcc ttgactgccg cggcgttgaa cgtgccccgg atcttgttca ccacgagtgt
     1501 ggccagagcc tcgccctcga tgtcctcggc caccagcagg aggggcttgc cggcctggag
     1561 caccttctcc agcagcggca gcagctcctg gacgctcccg accttcttgt tcaccaggac
     1621 gatgtagggc tcgtccagga ccgcctccat ccgctcctgg tccgtgacga agtacgggga
     1681 gatgtagccc ttgtcgaact gcatgccctc gacgaactcg agctccatgc cgaaggtgtt
     1741 cgactcctcg acggtgacca cgccgtcctt gcccaccttg tccagggcct cggccaggac
     1801 ctcgccgatc tgcgggtcgg cggccgagat ggcggccacc tgggcgatct cgtcccggga
     1861 ctcgatgtcc ttggcctgct ccttgatcac ctccaccgcg gcggccacgg ccttctcgat
     1921 cccccgcttc agcgccatgg ggttggcccc ggcggcgacg ttgcgcatgc cgtgacggac
     1981 catggcccga gccagaacgg tggccgtggt ggtgccgtcg ccggccacgt cgttcgtctt
     2041 ggtggcgacc tccttggcca gctgggcgcc catgttctcc cacgggtcct ccagctcgat
     2101 ctccttggcg atggtcacgc cgtcgttcgt gatggtgggc gcgccccact tcttgtccag
     2161 gaccacgttg tggcccttgg gaccgagggt cagggccacg gtgtccgcga gcttgttgac
     2221 cccggcctcg agcgagcgcc gggcctcctc gctgaacttc agctccttgc ttgccatggg
     2281 acctccaact cagatggttc gggggcccgg cgcgccgcgc gcgagccccc ggaacgtgcg
     2341 ttcgccgccg ctacttctgg acgacggcca ggacgtcgcg agcagagagg atcaggtagt
     2401 cgtcgcccgc caccttgacc tcggtgccgc cgtacttcga gtagatgacc ttgtcgccga
     2461 ccttcacgtc gagcgggacc cggttgccgt cctcgaaccg gcccgggcca acggccacga
     2521 cctcgccctc gaccggcttc tccttggccg tgtcggggat cacgatcccg gagaccgtgg
     2581 tctcctcctc ctcgcccggc ttgaccacga tccggtcctc gaggggcttg aggctgaact
     2641 ccttcgccat cccgtcctcc ttcgcaggcg tcctgcggtc ccggtcccgg ggcggacctc
     2701 gggcagggat ccgtgcgctc gtctagcact cgaccgcttc gagtgctaac aggatagcga
     2761 cgagagtgcc aggggtcaac gtggcgtggg ccgtcccggg ccggtcctgg tccatcgacc
     2821 gtagcaggcg ctcctgcctt cgtcggtggt gcggcccgct ccagggctcc tatcgtgggc
     2881 ccggaccggt tcgaggccgg ccacgaaggg ggacgatgcg gaaggggcga ggccaccgcc
     2941 gggcgatcac ggccgtcctg gcggccggga ccctcctgac aggctccctc accccttccg
     3001 cctccccagc cgcagcggcc gagcccgtcg gatcgctgca ggccacgatc gacacctcct
     3061 cgtggtcgcc gccgagcccc gaccccaccg ggatcacctg gtggaccgac cggctcctgg
     3121 tggtggacag cgaggtcgag gagacgatct cggggatcac ccactacgag ggcgcgaaca
     3181 cctacgaggc gtccccctcg ggcacgctgg agcggacctc caccaccacc gccttctcca
     3241 cggaacccac cgacgtcgcg gcgcacggct cccgggtgtt cgtgagcgac gacaaccggg
     3301 accggatctt cgtgatccac gtgggtccgg acgggcggtt gaacacctcc gacgacaccc
     3361 ggacctcgat cgggacccac gccttcggca gctacgacga cgaggccgtg tccttcgcgg
     3421 gcggggacct gttcgccggc gacgggacca acacccagat ctaccggatc gaccccggcc
     3481 cggacggggc cttcggcggc acggacgacc gtgtgacctg cttcgatacc cgggccctcg
     3541 gcatgcgcga tcccgagggc gtgacggtgg atccggccac cgggcggatc tacgtggcca
     3601 gccggatcag ccgagagatc gccgtggccg atgggtccgg tgccctgctg aacctgatcg
     3661 acctgcggcc gatcctcccc tacgggggcc gggaccggat cgcgccctcc ggggtgaccc
     3721 tggcgccggc cgccgaagac ccgtcccgca tcgacctgta cctcaccgac cgcggcgtgg
     3781 acaacaacac cgaccccagc gagaacgacg gcgcgatcta ccagatccgc cggcagagcc
     3841 cggacggcaa cgtcgcgccg gtcgtctccg gcttcggcgc cttgcgggca gtggagggcg
     3901 agcgcgtgag cgtccgcacg gtggcggccg atcccaacgg tgacccgctc tcgtactccg
     3961 ccaccggcct gccccccggg ctgtcgatcg acccctccag cgggctgatc accggcaagg
     4021 tggccgccgg agcggccgcc ggcagcccct acgtctcgca cgtgaccgtg agcgacggga
     4081 gcctggccac ggtccgccgg gcccggtggc ggatcaccac cggggccccc gacgatcggg
     4141 cccccgtcat ctcccccacc ggcgaccagc gcagcaccga gggagacccc gtcgccctgc
     4201 ggatccaggc ctccgatccc gatgtcgggc aggcattgat gtactcggcg acggggctgc
     4261 cgccgggcct gtggctggac tgcaccagcg ggcgcatccg gggccgggtg gcctccggcg
     4321 cggcgtcggg cagcccctat cgggtccggg tccaggtgga ggaccagacg atcccggcgg
     4381 atccctcctt caccagccgg ttcgaccagg agtccttcag ctggaccgtg agcgccccgg
     4441 gcccgtcgcc ctccatcacg ttccgggacg cctccaaggg gagcacgccc ggtccatcca
     4501 ggcgcctgcg gatccccagc cccagcgggg tggcccccgg cgacgtgctg gtggcggcga
     4561 tcgacgtgcg ggggccggcc cggatcggct cgccgcgggg atgggtccgt gttcgggccg
     4621 atcaccgggg acaccggctc cgccagttcg tctacgtccg cgtgctgacc ggcgatgagc
     4681 ccgggacctg gcgatggcgc ctgtcccggc gccagccggc cacggggatc atcgtgggct
     4741 accggggggt cgatcccatc gatccgatcg aggtcgcggc cgggagggtg aagcggcggt
     4801 cccggtggat caccgctccc tcggtcaccg ccgcggagcc ctcggccatg gtggtgggga
     4861 tgttcggggt ggccgccagg gcctggatcc ggccgccgcg gggcatggac gagcgggccg
     4921 agatccgcct ccggcgctcg agacggagga tcaccacgga ggtctccgac cggctcctaa
     4981 gcgacgccgg ccccaccgga cggctggtgg ccagggccac cagggccggg acgaacatcg
     5041 gccagagcct catcctccga ccggcgtcct gaccggccgt tcggcctaac cccccggtac
     5101 ggcgaggaac gtacccgagt tacggcggat gccgctcgga aacatctcgc ttcccaggaa
     5161 agctggtggt cggcgatgca gacccgacca tggcgcatgc tccgggggga cgttgtcgcg
     5221 ttcggcgacg tcgagaccga ggagatcgat cctcagggga cggctcaggc tgccctggag
     5281 ggggacggca tccgcctgca cggggcggga tggcggctgg tcaagcgcct gctcgacgtg
     5341 gtgctgagcc tcacgctgat ccttctgact ctcccgctga tgctcctgat cgccctggcc
     5401 atcacggccg agtcgcgcgg cccgctcttc ttcgtgcacc gccgcatggg caaggggcgc
     5461 gtgcccttcg ggttgctgaa gttccggacc atggtccccg acgcggagga gcggctggag
     5521 gcgcacctgg ccgagcaccc cgagcacctg gccgagtggc ggcagtacta caagctccgg
     5581 gacgatcccc ggatcacccg cgtgggcagg ctcctgcgca agtcgagcct ggacgagctg
     5641 ccccagctct tcaacatcct gcggggggac atgagcgtgg tgggaccgcg ggcgatcgtg
     5701 cgcgacgagg tcccacggtt cgagcggtac gccgacacga tgctgagcgt caagcccggg
     5761 ctgaccggcc tgtgggcggt gagcgggcgc aacgacgtca cgcagcagca ccgggccgtg
     5821 ctcgagtacc gctacgtgac cgactggagc ctcctgctgg acctgtggat cctgtttcgg
     5881 acgatccccg cggtgatccg cggacacggc gcctactgat ccggtgctct gccgaggtct
     5941 gcgaacctgg tagccgcccc ccgggggcgc cgctggccgg ccagctcccc tccggtctca
     6001 gcgaccggcg atcatcgacg tgagcgcggc atcgtaggcc cgggcggccg cgctcagcgt
     6061 gtacttctcc aggaacgcgg ctcggcctgc ctcgcccatc gcctcccggc gctcgggctc
     6121 cgcgaccagg cccctgatgg cccgggcgat ctcccgaccc tcgggctcga ccaggaggcc
     6181 gcagccggtc tccttgatca tctggccgat ctcgctgtcc ggatcgagga tgcccatgag
     6241 cggcgtcccg ccggcgatga ccccgtacgc cttgctgggc acgctgatgc cggtgtggcc
     6301 cgcctccagg cagaccaccg ccaggtccgc ggccgagagc atctcggcca gccgatgcat
     6361 cggctggtag ggcaggaact gcacgttgcg caggcggtgc tcggccgcca tggcctggag
     6421 cttgggcttc ttggcgccct ctcccacgaa ctggaacagc accggctccc cttccaggag
     6481 ccgagccgcg tcgatcaggg gctcgaggtt gtgcttctcc cccagccggc ccgcgtactg
     6541 caccacgaag cggtcctgca ggtggtgctc ggcccggaac gtgttctcct cgcgcgggat
     6601 cgggtgaacg tgccgctcgt ccgaccagtt ggggatcacc acgatccgat cgtgggtccg
     6661 ctcgggcatc ttccgcttga tcaggtcgag catgtcccgg ccgatcacca ccgtcacggc
     6721 cgcgccggcc aggatcagcc gggtggtccg ggcccagacc ttggccagcc aggagtccgc
     6781 ccgcagcacg cccaggctga cggcgaagtc ggggaacacg tcgtacacga tgatgaggta
     6841 gggccgtcgc cgcagtcggg agacgagcca tcccagcacg cccaggaacg gggggttggt
     6901 ggtgatgatc atcccggcgt gatcgctccg ctggcgcagg agggcccagg ccgcggacag
     6961 cacgaagctg accgaggcca cggtcttcga cacgagcccg ccgcgctggc tgccgatggt
     7021 gggcacccgg aggatcctca cgccctcgtg gacgagctcc ttgggcaccg cccggccgtc
     7081 gccctcgtcc gttccccagc tgggcttggc ggtgtagacg gtgatctccc accccagctc
     7141 ggccagccgg gtggccagct cagtcatgtg catcccggtg gagatcatct ccgggtagaa
     7201 gtgctcgcac agcaacagca gggtcttccg gggagcggag gctccaccgg gatccctggg
     7261 cgtcccggcg ctgccggcct caggtaccgc catggtccgt cccgtttcct cccgtcggtg
     7321 gtggcaacca cggcggcccc cgtgatgcgg cgaccgtggc cacccgccga ttgtggccga
     7381 ggacctcggc ccctgcatcc gtggaagcgc gtagcccgcc tagggaaacc gaccgagccc
     7441 tgcgcccggg gcgcgatgcc ccggtactgc ggctccctac ggcggatgcg cgatgagcgc
     7501 gcggcccgga ggagcgacga taccaccacc gatgagccag accggaacac cgatcaaggt
     7561 cctgtacgtc ctgggtccgg ggcggagcgg caccggcatc ctggggcggg tcctcagcac
     7621 catggagggc gccgtcttcg ccggggagct gcgccggctg tggagccgcg ggctgcggcc
     7681 gggacggacc tgcgcctgcg gacggcccca cgccgagtgc ccggtgtggt cccggctgct
     7741 ggtcccgggt gccccgttcg cccatccgag cctgcaggcc ctgggagcga tccaggctcg
     7801 ggtcgccccc gagcacctcg gatggcgcgc cggtctgcgc ctgcgccacc tccaggcgcc
     7861 tccggctccc gacacgcccg aggggcggta cctggccgcg tacacggcgc tgcacgaggc
     7921 gttcgcccgc gtcaccgggg cccaggtggt aatcgacagc tcgaagtcgg cggcggacgc
     7981 gtccctgctg gccgtgcggc gcgaggtccc gacgtccatc gtgcagatcg tgcgggaccc
     8041 gcgaggggtc gccttctccc tgcagcagca ctacgcgaag gacgcgagcg gtctgcgccg
     8101 gcacctgctg gcgctgcgcg gagcggtccg gtgggtggcc aagcacctga ccaacgaggc
     8161 cctgcgccgc cggtacggcc ccgatcgatc gatcgtcctg cactacgagc ggttgatcga
     8221 ggatccccgg agcgtggtgg aggaggtggc gaagaccgtg ggcctggccg cgccggcgat
     8281 cgagctgtcc ccgggggtcc ccatcgaggt cccggaggtc cacggccccg acggcagcaa
     8341 gcggcggcgg ttcgtgccca cccagatcgt cctgcgcctg gacacgcggt ggcaggccga
     8401 gctggacccc gtcgaccggt tcctggtgac cgcggtgacg ttcccgctcc tcctgcgcta
     8461 cggctatcct ctgcggaccc ccagaccgag cgcctcgacg acgacccggc cggggaccga
     8521 ggtcgagggg tgaggagagc ggacgtgggg gtccaacggg gcacggagcc ggatccctcc
     8581 cggccggaga acaggacggg atcgctggac gcgggccctt cctcgaagct gcccaccttc
     8641 ctcctgatcg gggccatgaa ggcgggcacc acgagcctgt accactacct ccgcggccac
     8701 ccgcaggtgt tcatgccgga ggtgaaggag gtcatgttct tcgaccctcg gcacaactgg
     8761 caccgcggga tcgagtggta cgaggcccag ttcggcgggg ccgaggctcg gcacctcgcg
     8821 cggggtgagg cctcgaccag ctacacgaag ttcccggtgg tccagggggt gccggcccgg
     8881 atcgcctcgg tcctgccaga cgttcggctc ctgtacgtgg tccgtcaccc ggtcgagcgg
     8941 atgcagtcgc actacctgta cctgctctcc cggggcaagg agtctcggcc catcgagcga
     9001 gccttcgccg aggagccgat gtacctggac accagccgct acgcgctgca gctggagcag
     9061 tacgagccgt acatccggcc ggagcagctg ctgctgatcg actcgcgtga cctgcgggac
     9121 cgccgggtgg agacgttggg cggcatcttc gcgttcctgg gggtcgatgc cacctgggtg
     9181 ccacccaccg tcgatcggga gttcttccgc tcggccgacc gtcggatgcg ccccgcgctg
     9241 tcccgagccg tgcgtcggct gcccggcctg tcggcggccg cccggcgagc gccccgtccg
     9301 cttcgg
//
LOCUS       DASQQQ010000311        14435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_21559, whole genome shotgun sequence.
ACCESSION   DASQQQ010000311 DASQQQ010000000
VERSION     DASQQQ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14435
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21559"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VE646_11660"
     CDS             complement(<1..703)
                     /locus_tag="VE646_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(455..457),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZD80679.1"
                     /translation="MGDGYARLTEPMVRQNGSLRAATWEEALDRAAERLRRATERNGG
                     AGVGVFSCSKATNEMNYLAQKFVRQSLGSNNIDSCNRTUHAPSVVGLATVFGAGGGTS
                     SYEEIEDTDLIILWGSNARETHPIFFHHILKGVRRGARLYAVDPRRTSSAQWADVWLG
                     LDVGTDIALANTMGREIIHAGLTNDTFIERATEGFEAYAAAVEPWTLEEGERATGVPA
                     DAIRGFAHAYARADRA"
     gene            complement(785..1627)
                     /gene="fdhD"
                     /locus_tag="VE646_11665"
     CDS             complement(785..1627)
                     /gene="fdhD"
                     /locus_tag="VE646_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HZD80680.1"
                     /translation="MAQAVPLRRTITRTRVVAVRGDRADERPDRLAAEEPMEIRVQGP
                     GQDAVSVAVTMRTPGGDFELAAGFLFTEGLIAGPEELRTVSYCRDLAPEDQHYNVVTV
                     SLAAAFDRDRLSRNFFATSSCGICGKAALEHVEVRCAPVGPGPTVRGSVIGGLPAELR
                     RAQSVFDQTGGLHAAGLFGIDGVLLSLREDVGRHNAVDKVIGEALLARSLPLSERILL
                     VSGRASFEIVQKAAVAQIPIVAAVSAPSSLAVDTADRFGMTLLGFLRDDRFNVYTRPG
                     RIVV"
     gene            1779..3449
                     /locus_tag="VE646_11670"
     CDS             1779..3449
                     /locus_tag="VE646_11670"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HZD80681.1"
                     /translation="MKSGLQIAQEAELRPVAEVARAAGIEDEELELFGRFRGKVSLSV
                     LDRLADRRDGKLIIVTAITPTRAGEGKTTTSVSLTQGLGAIGKDVMLCLREPSMGPVF
                     GIKGGGNGGGHAQVVPMEEINLHFNGDFHAVTAAHNLLAAALDASIYHGNPLGIDAAT
                     ITWPRTLDVNDRELRYTVIGLGGKAHGVPREASFVITAASEIMAILALASDLRDLRRR
                     LGRIVVATTLDGRPVTAEDLKVAGAMTVVMRETIRPNLVQTLEGQPVLIHTGPFGNIA
                     HANNSIVADRIALKLADYVVTEAGFGSDLGFQKFADIVCRLGGFAPSAGVLVTTVRAL
                     KSHGGVSFPDLGSEDLDALRRGTENLAAHVEIVRAYGVPCVVSVNRFPTDTDAELAAI
                     DELAAKVGAERVVRNEGFARGGEGAAELAHAVVEACERPNTFSPLTAPGTSVRDQIEA
                     IAVKLYGAEGVDLSPKAERDLATIEALGLGQAPVCMAKTHLSLSHDPTLLGRPAGFRL
                     PVRGLVPSAGAGFVVVLCGDMQRMPGFGKVPNFTGVDIDERGRTVGLF"
     gene            complement(3468..3659)
                     /locus_tag="VE646_11675"
     CDS             complement(3468..3659)
                     /locus_tag="VE646_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80682.1"
                     /translation="MHSCAPPKDADPRAQTLWVCAGCGSVWEAAPEQGGIFDFERDDV
                     VARAEWILLEEGSWTSLAG"
     gene            complement(3752..4852)
                     /locus_tag="VE646_11680"
     CDS             complement(3752..4852)
                     /locus_tag="VE646_11680"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HZD80683.1"
                     /translation="MADEPVRSIWHEVQERQGATFEDFDGWLWTESFGDVGKEYEAIR
                     KDVAMWDVYPLVKWDFRGADAVRAAQRISTNDILGLGVGRVRYGAFVDENGQMVDDGT
                     VYRLAEDHLWVMTNAPGYEDWFGEVFAGMDVVVENRTTQMPLLSIQGPRSREVLQGLT
                     QADLSSLGYFHLWPNRVALAGIDVWVLRTGFSGELGFELIPAPEDAVALWEAVQGAGV
                     TPFGTAAVEIARIESGMIVAGVEYEKGTRSPFDLSMDRFVALDGQGEFLGKARLEQIA
                     AAPPNRFKTLRVQGTQVPEYGATVTKGGEEIGTMTSATDSPLFGVIGLAVLDSNHAAE
                     GTEVEVGLGEGTVPATVDVLSVYDPNKERPRS"
     gene            complement(4861..5970)
                     /locus_tag="VE646_11685"
     CDS             complement(4861..5970)
                     /locus_tag="VE646_11685"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HZD80684.1"
                     /translation="MTEEEAIRTAFHDVTEAQGGTHVADGGWLWLEGFGDVRKEYDAV
                     RDHVAVWDVSPLNKWDFRGPDALRAAQHLFSNDAMSLAVGQARYGAFLDGDGLMVDDG
                     TVFNTAREDHCWVMTNGKDHQEYFGEALKGFDVGFEWIASRMPHLGVIGPRSRELVGS
                     LTDADVASLKYFRFLPEPVTVGGVRVYLSRTGFGGEMGFELFLTDPADAADLWNAVVA
                     AGATPFGVEAIEILRIEAGLIVTDYDYEAHQRTPYDFDLDRLVALDRPVRFQGKDALA
                     KIAPHPPNRFVTLRYEADELPEYGAAVTGDGEEVGTLTSPTDSPRFGKIGLAVIRADL
                     ATRGTEVSVALGEGTVPATVDVLPIYDTNKERPRA"
     gene            complement(6035..6700)
                     /locus_tag="VE646_11690"
     CDS             complement(6035..6700)
                     /locus_tag="VE646_11690"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HZD80685.1"
                     /translation="MPLRSRRRRPAGPSRAARRLDRRQAEMVALDFETTGLDRERDQV
                     VSFGAVPVREGRVMVGEAVQRLISPTVPASAASMRIHGLLPRDLADAPPLSAVRSELS
                     DVLEGRFILAWFADVEIAFLSRVFPVPARRWIRRTIDVRLLALALEGAGRAARRTLSD
                     TAVRYGVPVETPHDALEDALVTAELFLVLASRLEARGRVRMSDLLGLCRGAEARRRAS
                     PPT"
     gene            complement(6691..7272)
                     /locus_tag="VE646_11695"
     CDS             complement(6691..7272)
                     /locus_tag="VE646_11695"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HZD80686.1"
                     /translation="MNTADRLKRDKRALRAQIRELRDRLSPPERDRLSRAIARELFAL
                     PEMERADVVMAFSSFGSEVDTGPILERLSARGRRLALPRVRDGDIEGLAFAPGQELRQ
                     ARFGALEPVDGAPVAPEEVDLVVTPGVAFDRRGFRVGYGGGFYDRFFRRTRSDVVKAA
                     VCFGLQVVERVPHGAQDLPVDLLVTEDGVILCR"
     gene            complement(7269..8189)
                     /locus_tag="VE646_11700"
     CDS             complement(7269..8189)
                     /locus_tag="VE646_11700"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZD80687.1"
                     /translation="MSDLGGRMRVGAMLPLFVDDPARVIGFARRAEALGFDGVFAFDH
                     LLPLHQAPDRPSFEVFSSLAAVAAATDGLTVGTLVTRASLRPAGLLAKLAASLDDVAA
                     GRVVLAVGTGDDLSRGEHEAFGIPTMDRATRRRHLEDTVSAVRSLLTGRTWPGSELVP
                     PLSGPLLPRRPGGQGPPVWVGGLSEEVVRVAGRVADAWNGWGIDVDGFARRVAVLHEE
                     AEAAGRTVPATWGGIALVGRDRAEADRLAERRGERPSGAWVGDPRGAADRLQRLAAAG
                     ATWAVMLLAGPADRLEVVGREVLPRLRDGS"
     gene            complement(8209..9117)
                     /locus_tag="VE646_11705"
     CDS             complement(8209..9117)
                     /locus_tag="VE646_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZD80688.1"
                     /translation="MKVGVVLPLFSGDAARVLDTARRAEDLGYDGVFAFDHFFPPGAP
                     GDRASLEAFATLSAVAAATRRVTVGTLVTRASIRPAGLLAKLVASVDDIGGGRTVLGI
                     GTGDAIDRPEHETFGLPYLGIRDRREHLVEAVRALRTLLRGDPWPGGARVPALSGPLA
                     PGVRAPGGPPIWIGGQADEVVRIAAREADAWNGWGLSIPEFARKAAVLREEAAEAGRS
                     VEATWAGIAVVGRDEDEAGRMLDERANRGMLETNVWGGSVQALTWWFEGLETAGARWV
                     VLVPAGPPNRIELIAERVLPEMRSRT"
     gene            complement(9146..10102)
                     /locus_tag="VE646_11710"
     CDS             complement(9146..10102)
                     /locus_tag="VE646_11710"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HZD80689.1"
                     /translation="MGAGAGQALGIDVGGTKIACLRVSRSGAILARDTVPTPAEDPEA
                     TMEAMILVAKSLLGPEVVAVGVGAAGMVDANLGVLRFAPNLAWRELPIARRIGEALGL
                     PCQVDNDANVAAWGEWRFGAGRGFRHMLLVTVGTGIGGGIVADGRLFRGAHGFAAEIG
                     HIVVEPDGPMCGCGNRGCWEQVASGTAIGRLGRRAVTEHPDSAVATLAQGRPDAVTGR
                     LVTEAAHRGDPVAAGILAEVGRRLGEGIAGLVNALDPQVVVVGGGAVEAGDLLLEPAR
                     STYWPSVEGPDHRPRVPIVAAELGNDAGAVGAAALALEELGA"
     gene            complement(10110..11237)
                     /locus_tag="VE646_11715"
     CDS             complement(10110..11237)
                     /locus_tag="VE646_11715"
                     /inference="COORDINATES: protein motif:HMM:NF037526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HZD80690.1"
                     /translation="MMSLDHTLLRTLAGWDSAGLPVTSLYLTVDGRRYPRRSGYEVRL
                     DELLRQARSRSEVLGPEALRWIEQDLGAISSYVREEFERGDTRGLALFSSRGAGLWEE
                     VRVPRPVRDRAVAGPRADLVPLEQVLDTYHPICAVLVDHARARLFVCELGRIREVAAV
                     EGEVPGRHDQGGWAQMRLQRHVDDRRQKHLKAVADTVFRTFKARPFDHLVLAGPAEAH
                     LELERELHDYLRQRVRARITVPMTASPHDVAARCVELEEALERERERALVDRLSGSAP
                     SGVQAITGLPRTLRALAESRVRELVVWVDLASPGASCATCGRLAEREGRCATCGGTLE
                     PVPDVVEAAVAEALHQGCRVETVVDGTDLAPMGGIGALLRF"
     gene            complement(11234..11527)
                     /locus_tag="VE646_11720"
     CDS             complement(11234..11527)
                     /locus_tag="VE646_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80691.1"
                     /translation="MAETRTRARRSTGGRSRAAEPPPEPAAGQPEEHVCPVAFCPIGM
                     ALTAVNRSNPEALEHLILAARELLFAVRSVIDVRADDLTDREGRSDLERIEIA"
     gene            complement(11520..12734)
                     /locus_tag="VE646_11725"
     CDS             complement(11520..12734)
                     /locus_tag="VE646_11725"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HZD80692.1"
                     /translation="MRVLLFTGKGGVGKTTAAAATAVRAARSGNRTLVMSTDPAHSLA
                     DSFDTEVFDAPTPVAPLLWAEQIDAQARLETNWREIQEYMVQLMNWAGVETIQAEELS
                     VIPGLDEIFSLIDVKRHVDDARYDLLVVDCAPTAETLRLLSLPEIMNWYIERIFPVQR
                     RVTKAVRPMMSRMTSLPIAGDRVYTAVERLHRNLEGVHRILTDERSSSVRLVVNPEKM
                     VIAEARRTYTYLGLFGYRVDAIIVNRIIPEDVTDPYFGKWKDIQAEHLQTVRESFEPV
                     PILTARLFDREMVGVDLLDDMGAEIYGERPATDVLHRDDPIRVRRRAGGYALSMRLPF
                     VSRDDMDIHRRGDELFVRVGSYKRNLILPQSLQRLEVAGANFVEDRLEVLFVRQLAPD
                     PSGGGRKERTHG"
     gene            complement(12775..13218)
                     /locus_tag="VE646_11730"
     CDS             complement(12775..13218)
                     /locus_tag="VE646_11730"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZD80693.1"
                     /translation="MGEHAEGSIVIGASAAEVMDVITDYEAYPEWADVKSARVVQRGA
                     GGLGTEVAFEVDVPVLGRASYTLAYRYAPGDAGLSWVTKEARGAVSNISGEYLLDELD
                     ARHTRVTYRLSVDLGVLLPGFVRTQGAKRVIENALEGLKRRVELG"
     gene            complement(13241..14011)
                     /locus_tag="VE646_11735"
     CDS             complement(13241..14011)
                     /locus_tag="VE646_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger Ran-binding domain-containing
                     protein"
                     /protein_id="HZD80694.1"
                     /translation="MVADRRCPRCGALVAEDAEWCGQCYASLVEPSPARREAAPASRE
                     ATDPRAATWGGRAAAEAPPSMAGTPPPMPGQPQPPHAPQDEAFPTGAGASAADARWPC
                     PVCQHQNPIHLDACEVCGTPFARLFQEQERPPEVEPGTAALWSALFPGLGHWRCGRTL
                     DGVARAVIFAWTFGTLLILILSRIGKGGLGATLPLFALFLASSVAVWMSAVLDAYRIA
                     AGADPVVSSRTLLWGSVVLIVVSVLLASLVTLPAVRGG"
     gene            complement(14085..>14435)
                     /locus_tag="VE646_11740"
     CDS             complement(14085..>14435)
                     /locus_tag="VE646_11740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZD80695.1"
                     /translation="PRYYAAGDVFAMPCRTRLGGLEVEGWGNVFIEAAACGRPVVVGD
                     SGGARESLVDGTTGVLVDGTRVAEVADAVGALLADPGLARRMGEAGRRRVERSYTWPR
                     AAAQLSGWLAAAAG"
BASE COUNT         2102 a         5563 c         4830 g         1940 t
ORIGIN      
        1 gcgcgcgatc ggcgcgggcg taggcatgcg cgaacccccg gatggcatcg gccgggacgc
       61 cggttgcccg ctcgccctcc tccagggtcc agggctccac cgccgccgcg tacgcctcga
      121 agccctccgt ggcccgctcg atgaaggtgt cgttggtgag cccggcgtgg atgatctcgc
      181 gccccatggt gttggccagg gcgatgtccg tgcccacgtc cagccccagc catacgtccg
      241 cccactgggc cgagctcgtg cgccgcgggt ccaccgcata gagcctcgcg ccgcgccgca
      301 cgcccttcag gatgtggtgg aagaagatcg ggtgggtctc ccgggcgttg gacccccaca
      361 ggatgatgag atccgtgtcc tcgatctctt cgtacgagct ggtgccccca ccggccccga
      421 acaccgtggc cagaccgacc acgctcggag cgtgtcaggt gcggttgcag ctgtcgatgt
      481 tgttgctgcc gagcgactga cggacgaact tctgggccag gtagttcatc tcgttcgtcg
      541 ccttcgagca gctgaacacg ccgacgccgg ccccgccgtt ccgctcggtc gcccggcgca
      601 accgctcggc ggcccggtcc agggcctcct cccaggtggc ggcgcgcagg gatccgttct
      661 ggcggaccat cggctcggtc aggcgggcgt acccgtcgcc catcgagctg gcctccttcc
      721 tgaacccacg ttcgcccatc ttatcccccg gttcaaccga gggggtccgg ccgcgggggg
      781 cggctcagac gacgatccgc cccggacgcg tgtagacgtt gaaccgatcg tcccggagga
      841 accccagcag ggtcatgccg aaccgatcgg ccgtgtccac ggccagggac gatggcgccg
      901 agaccgcggc cacgatgggg atctgggcaa ccgcggcctt ctggacgatc tcgaaggagg
      961 ctctgccgga caccaggagg atccgctcgg agagcggcag gctcctggcg agcagcgcct
     1021 cgccgatcac cttgtccacg gcgttgtgcc gccccacgtc ctcacgcagc gacagcagga
     1081 cgccgtcgat accgaacagc ccggccgcgt gcagcccccc ggtctggtcg aacacgctct
     1141 gcgcccggcg cagctccgcc gggagaccac cgatcacgct cccccgcacg gtaggcccag
     1201 gacccacggg ggcgcaccgg acctccacgt gctccagggc ggccttgccg cagatgccgc
     1261 aggaggaggt ggcgaagaag ttccgggaca ggcggtcccg gtcgaaggcc gcggccaggc
     1321 tcacggtgac cacgttgtag tgctgatcct ccggggccag gtcccggcag tagctgaccg
     1381 tccgaagctc ctcgggcccg gcgatcaagc cctcggtgaa caagaatccg gccgcgagct
     1441 cgaagtcgcc gcccggggtc cgcatggtga cggccaccga caccgcgtcc tgaccgggac
     1501 cctggacgcg gatctccatg ggctcctcgg cggccagccg gtcggggcgc tcgtccgccc
     1561 ggtcaccgcg caccgccacc acgcgggtcc gggtgatggt gcgacggagc gggacggcct
     1621 gcgccatctg tccatcatcc caccggcgcg gcgatgtttc ccatctccac gatggggttc
     1681 gggcgccgtg acctgcggtc acgcctcagc ggacccctcc ggggtatcct cacagcggtc
     1741 atttgaacgg ccggtcaaag gcgggggtac cgggatcgat gaagagcggg ctgcagatcg
     1801 cgcaggaggc ggagcttcgt cccgtcgccg aggtggctcg ggctgcgggc atcgaggacg
     1861 aggagctcga gctcttcggg cgcttccgcg gcaaggtctc gctctcggtc ctggaccggc
     1921 tggccgaccg ccgggacggc aagctgatca tcgtcacggc catcaccccg acgagggccg
     1981 gtgagggcaa gaccaccacg tccgtctcgc tgacccaggg gctgggagcg atcggcaagg
     2041 acgtgatgct gtgcctgcgc gagccctcga tggggccggt gttcgggatc aaggggggcg
     2101 gcaacggcgg gggccatgcc caggtggtcc ccatggagga gatcaacctg cacttcaacg
     2161 gcgacttcca cgcggtgact gcggcccaca acctgctggc cgccgctttg gacgcctcga
     2221 tctaccacgg gaacccgctg gggatcgacg cggccacgat cacctggccc cgcacgctgg
     2281 acgtgaacga ccgcgagctg cggtacacgg tgatcggctt gggcggcaag gcccacggcg
     2341 taccccggga ggcctccttc gtgatcacgg cggcatccga gatcatggcc atcctggccc
     2401 tggcctccga tctgcgagac ctccgacgac gactggggcg gatcgtggtg gccaccaccc
     2461 tggatggccg gccggtcacc gccgaggacc tcaaggtggc cggagcgatg acggtcgtga
     2521 tgcgggagac gatccgtccg aacctggtcc agaccctcga gggccagccg gtgctgatcc
     2581 acaccggccc cttcggcaac atcgcccacg cgaacaattc gatcgtggcc gaccggatcg
     2641 cgttgaagct cgctgactac gtggtgacgg aggccgggtt cggatcggac ctggggttcc
     2701 agaagttcgc cgacatcgtc tgccgcctcg gcggcttcgc tcccagcgcc ggggtgctgg
     2761 tcaccacggt ccgagccctg aagtcccacg gaggcgtatc cttccccgac ctgggctccg
     2821 aggacctgga tgcgcttcgc cgggggacag agaacctggc cgcccacgtg gagatcgtcc
     2881 gggcctacgg cgtgccctgc gtggtctccg tgaaccgctt ccccaccgac accgatgccg
     2941 agctcgcggc catcgacgag ctcgccgcga aggtcggtgc cgagcgggtg gtccgcaacg
     3001 agggcttcgc tcggggcggg gagggagctg ccgagctggc ccatgccgtg gtggaggcct
     3061 gcgaacgacc gaacacgttc tcgccgctca ccgcgccggg gacctcggtc cgcgaccaga
     3121 tcgaggccat cgcggtaaag ctctacggag cggagggggt cgacctctcg ccgaaggcgg
     3181 agcgggacct ggcgacgatc gaggcgctcg gcctggggca ggcgccggtc tgcatggcca
     3241 agacccatct gtcgctctcg cacgatccga cgctgctcgg ccggcccgcc ggcttccgcc
     3301 tgccggtgcg cggtctggtg ccctcggccg gggcgggctt cgtggtggtt ctgtgcggcg
     3361 acatgcagcg catgcccggg ttcggcaagg tcccgaactt caccggcgtg gacatcgacg
     3421 agcggggccg gacggtcggc ctgttctaga cgaccgccga ggccctgtca tccggccagg
     3481 gaggtccagg acccctcttc cagcaggatc cactcggctc gggccacgac gtcgtcgcgc
     3541 tcgaagtcga agatgccgcc ctgctccggc gcggcctccc acaccgatcc gcagccggca
     3601 cacacccaga gggtctgggc cctggggtcg gcgtccttcg ggggcgcgca gctgtgcatc
     3661 tgggctcctc tctgcagaat cacaagcgtg tgatccagca gcatagcggg gggaccgtgc
     3721 gcataccacg acctcggagg aaccgctgag gctaggagcg gggccgctcc ttgttcgggt
     3781 cgtagacgga gagcacgtcg accgtggccg ggaccgtgcc ctcccccagc cccacctcga
     3841 cctcggtccc ctcggccgcg tggttcgaat ccaggaccgc gaggccgatc accccgaaca
     3901 gcgggctgtc ggtggcgctg gtcatcgtgc cgatctcctc gccgcccttg gtcaccgtgg
     3961 cgccgtactc ggggacctgc gttccctgga ccctgagcgt cttgaagcgg ttcggggggg
     4021 cggccgcgat ctgctccaat cgagccttgc ccaggaactc gccttggccg tccagcgcca
     4081 cgaaccggtc catggacagg tcaaacggcg agcgcgtgcc cttctcgtac tccaccccgg
     4141 ccacgatcat cccgctctcg atccgcgcga tctccaccgc cgcggtcccg aagggcgtga
     4201 cgccggcgcc ctggacggcc tcccacaggg ccaccgcgtc ctccggagcc gggatcagct
     4261 cgaagccgag ctccccggag aagccggtgc gcaggaccca gacgtcgatc cctgcgaggg
     4321 ccacgcggtt cggccagaga tggaagtacc ccagggagga caggtcggcc tgggtcagcc
     4381 cctggagcac ctcacgggac ctcggaccct ggatcgacag caggggcatc tgggtcgtcc
     4441 ggttctcgac cacgacgtcc atccctgcga agacctcgcc gaaccagtcc tcgtagccgg
     4501 gagcgttggt catcacccac aggtggtcct cggccaggcg gtacacggtg ccgtcgtcca
     4561 ccatctgccc gttctcatcc acgaacgcgc cataccggac ccggcccacg cccaggccca
     4621 agatgtcgtt cgtggagatg cgctgggccg cccggaccgc gtcggcgccc cggaagtccc
     4681 acttcaccag gggatagacg tcccacatgg cgacgtcctt gcggatcgcc tcgtactcct
     4741 tgccgacgtc gccgaaggac tcggtccaca gccacccatc gaagtcctcg aaggtggccc
     4801 cctgccgctc ctggacctcg tgccagatcg accgtaccgg ttcgtccgcc atcgctcctc
     4861 ctacgccctg gggcgttcct tgttggtgtc gtagatcggc agcacgtcga ccgtggcggg
     4921 caccgtgccc tcgcccagcg ccaccgagac ctcggtcccg cgtgtggcca ggtcggcgcg
     4981 gatcaccgcc agcccgatct tgccgaaccg cgggctgtcc gtggggctgg tcagcgtgcc
     5041 cacctcctca ccgtccccgg tgacggcggc gccgtactcg ggcagctcgt cggcctcgta
     5101 tcggagcgtc acgaaccggt tcggggggtg cggcgcgatc ttggccagcg cgtccttgcc
     5161 ctggaaccgc accggccggt cgagcgccac cagccgatcc aggtcgaagt cgtagggcgt
     5221 gcgctggtgc gcctcgtagt cgtagtcggt cacgatcagg ccggcctcga tccgcaggat
     5281 ctcgatggcc tccaccccga acggcgtggc cccggccgcc accacggcgt tccagaggtc
     5341 ggccgcgtca gccggatcgg tgaggaacag ctcgaacccc atctcccccc cgaacccggt
     5401 ccgggacagg tagacgcgga ccccgcccac ggtcaccggc tcgggcagga agcggaaata
     5461 cttcagcgag gccacgtcgg cgtccgtgag ggagcccacc agctcccgag accgaggtcc
     5521 gatcacgccc aggtgcggca tccgcgacgc gatccactcg aaccccacgt cgaagccctt
     5581 cagcgcctcg ccgaagtact cctggtggtc cttgccgttg gtcatgaccc agcagtggtc
     5641 ctcgcgcgcc gtgttgaaca ccgtgccgtc gtcgaccatg agcccgtcgc cgtccaggaa
     5701 cgccccgtag cgcgcctgcc ccaccgccag gctcatcgcg tcgttgctga acaggtgctg
     5761 cgcggcccgc aacgcgtcgg gaccgcggaa gtcccacttg ttgaggggcg agacgtccca
     5821 gaccgccacg tggtcacgga ccgcgtcgta ctccttgcgg acgtcgccga acccctccag
     5881 ccacagccac ccaccgtccg cgacgtgggt gcccccctgg gcctcggtca cgtcgtggaa
     5941 cgcggtcctg atcgcttcct cttccgtcat gtcctgcgcc tccggggctt ccctacgggt
     6001 gcaggcgcat cctcccacgc gattgactcg ccgttcaagt cggcggcgac gcccgacgtc
     6061 gagcctccgc gccccggcac aggccgagca gatccgacat cctgacccgg ccccttgcct
     6121 ccagccggga ggccaggacc aggaacagct cggccgtgac cagcgcgtcc tccagggcgt
     6181 cgtgcggcgt ctccaccggc accccgtacc gaaccgccgt gtccgagagc gtccgccggg
     6241 ccgctcgacc cgctccctcc agggccagcg ccagcagacg aacgtcgatg gtccgccgga
     6301 tccaccggcg ggccggcact gggaacaccc tggacaggaa ggcgatctcc acgtcggcga
     6361 accaggccag gatgaaccgc ccctccagga cgtccgagag ctcggagcgg acggcggaca
     6421 ggggtggagc atcggccagg tctcggggca ggagcccgtg gatcctcatc gaggcggccg
     6481 aggccggaac cgtgggcgag atcaagcgct ggaccgcctc ccccaccatc acccgtccct
     6541 cccggaccgg gacggcgccg aacgagacga cctggtcgcg ttcccggtcc agaccggtcg
     6601 tctcgaagtc cagagcgacc atctcggcct gccgccggtc caagcgccgg gcggcccgcg
     6661 aggggccggc ggggcgcctt cgccgtgacc tcagcggcac aggatcacgc catcctcggt
     6721 gaccagcagg tccaccggga ggtcctgagc cccgtgaggc accctctcga cgacctgcag
     6781 cccgaagcag acggccgcct tcaccacgtc cgagcgggtc cgacggaaga agcggtcgta
     6841 gaaccccccg ccgtagccga cccggaagcc ccggcgatcg aacgcgaccc cgggggtcac
     6901 caccaggtcc acctcctcgg gcgccaccgg cgcaccgtcg accggctcga gcgctccgaa
     6961 acgcgcctgg cgcagctcct gtccgggagc gaaggccagg ccctcgatgt cgccgtcgcg
     7021 gacccgggga agggccaggc gacggcctct agcggacagt cgctcgagga tcggccccgt
     7081 gtccacctcc gatccgaacg aggagaaggc catgaccacg tccgcccgct ccatctcggg
     7141 cagggcgaag agctcccggg cgatggcccg gctcagccga tctcgctcgg gagggctcag
     7201 tcgatcgcgg agctccctga tctgggcccg cagggcgcgc ttgtcccgct tcagccggtc
     7261 cgccgtgttc acgatccgtc ccggagccgc gggaggacct cccggcccac cacctccagc
     7321 cggtccgctg gaccggccag gagcatgacc gcccaggtgg cccccgccgc cgccaggcgc
     7381 tggagccggt cggccgcccc tcgcggatcg cccacccacg cgcccgacgg gcgctccccc
     7441 cgtcgctccg ccaggcggtc ggcctcggcc cggtccctgc ccaccagggc gatgccgccc
     7501 cacgtggcgg gcacggtgcg ccccgcggcc tccgcctcct catgcagcac cgccacccgt
     7561 cgtgcgaacc cgtccacgtc gatgccccag ccgttccagg cgtccgccac gcggccggcg
     7621 accctcacca cctcctcgct caggccgccc acccagaccg gcggtccctg cccgccgggg
     7681 cgacgcggca ggagggggcc cgagaggggc ggcaccagct cgctgcccgg ccaggtccga
     7741 cccgtcagca gcgaccggac cgcgctcacc gtgtcctcca ggtgccggcg gcgcgtggcg
     7801 cggtccatgg tggggatccc gaacgcttca tgctcgcccc ggctaaggtc gtcgcccgtc
     7861 cccaccgcca ggaccacacg cccggcggcc acgtcgtcga gcgacgctgc gagcttggcc
     7921 agcaggcccg ccggccgcag ggaggcgcgc gtcacgagcg tccccacggt tagtccgtcg
     7981 gtcgcggcgg ccaccgccgc cagggacgag aacacctcga aggacggccg atccggggcc
     8041 tggtgcagcg ggagcaggtg gtcgaaggcg aacacgccgt cgaagccaag ggcttcggct
     8101 cgacgggcga acccgatcac ccgcgccggg tcgtccacga acagggggag catcgccccc
     8161 acccgcatcc ggcctccgag atcgctcacc ggctcgtcct cgccacgatc acgtccgcga
     8221 ccgcatctcc gggagcaccc gctcggcgat cagctcgatc cggttcggcg gtcccgccgg
     8281 cacgagcacc acccaccgcg cccccgcggt ctccagcccc tcgaaccacc aggtgagggc
     8341 ctggaccgac ccgccccaca cgttcgtctc caacatgccg cgattggccc gctcgtcgag
     8401 catccgcccg gcctcgtcct cgtcccggcc gaccaccgcg atgccggccc aggtcgcctc
     8461 gaccgagcgt ccggcctccg ccgcctcctc ccggagcacg gccgccttcc gggcgaactc
     8521 ggggatcgac aggccccatc cgttccaggc gtccgcctcg cgggccgcga tgcgcacgac
     8581 ctcgtccgcc tgcccgccga tccagatcgg cggtccgccg ggggcccgga ccccgggagc
     8641 gagggggccg gacagggccg gcacgcgcgc ccctcccggc caggggtctc cgcggaggag
     8701 cgtccgcagg gcgcggaccg cctccaccag gtgctcccgc cggtcccgga tccccaggta
     8761 gggcaggccg aacgtctcgt gctcgggacg gtcgatcgcg tccccggtcc cgatcccgag
     8821 caccgtccga ccgcccccga tgtcgtcgac ggacgcgacg agcttcgcca gcaggcccgc
     8881 cggccggatc gaggccctgg tcaccagcgt gcccaccgtg accctgcggg tcgccgccgc
     8941 caccgcggac aaggtggcga aggcctccag ggaggcccgg tccccaggcg ccccgggcgg
     9001 gaagaagtga tcgaaggcga agacgccgtc gtagccgagg tcctccgccc gacgtgccgt
     9061 gtcgagcacc cgggcggcgt cccccgagaa cagcggcaag accacaccca ccttcatgag
     9121 ctccggcctc caggtcccac cgcggctagg cccccagctc ctcgagggcc agggcggcgg
     9181 cgcccaccgc gcccgcgtcg ttgccgagct cggccgccac gatcgggacc cggggccggt
     9241 gatcggggcc ttccacggac ggccagtagg tggagcgggc cggctcgagc aggaggtcgc
     9301 cggcctccac ggcaccccct cccaccacca ccacttgcgg atcgagcgcg ttcaccagcc
     9361 ccgcgatccc ctcccccagc cgtcgaccga cctcggccag gatgcccgcc gccacggggt
     9421 cgccgcgatg agccgcctcc gtcaccagcc ggccggtcac cgcgtcgggc cgcccctgcg
     9481 ccagggtggc gacggccgag tccgggtgct cggtcacggc ccgccggccg agccgcccga
     9541 tcgccgtccc cgaagccacc tgctcccagc agccgcgatt cccgcagccg cacatcggcc
     9601 cgtcgggctc caccacgatg tgcccgatct cggcggcgaa cccgtgggct ccgcggaaca
     9661 aacggccgtc cgccacgatg cccccaccga tcccggtccc caccgtcacc aggagcatgt
     9721 gtcggaagcc gcgtcccgca ccgaagcgcc actcgcccca cgccgcaacg ttcgcgtcgt
     9781 tgtcgacctg gcacggcagg ccgagggcct cgccgatccg ccgggcgatg ggcagctccc
     9841 gccaggcgag gttcggcgcg aagcgcagga cccccaggtt cgcatcgacc atgccggccg
     9901 cgcccacccc gaccgcgacc acctctggcc ccagcagcga cttggcgacc aggatcatgg
     9961 cttccatcgt ggcctcggga tcctctgccg gcgtgggcac cgtgtcgcgg gcgaggatcg
    10021 cgccgctccg ggacacccgc aggcaggcga tcttcgtgcc gccgacgtcg atccccagcg
    10081 cctgccccgc cccggccccc atcaccgcct cagaacctga gcagggcgcc gataccgccc
    10141 atcggggcca ggtccgtgcc gtccaccacg gtctccaccc ggcacccctg atgcagggcc
    10201 tccgccaccg ccgcctcgac cacgtcgggg acgggctcga gggttccgcc gcacgtggcg
    10261 cacctgccct cacgctcggc caggcggccg cacgtagcgc aggacgctcc gggcgatgcc
    10321 aggtcgaccc agacgaccag ctcccggacc cggctctccg ccagggctcg cagggtccga
    10381 ggcagacccg tgatcgcctg cacgccggac ggcgccgatc ccgagagccg gtcgaccagc
    10441 gcccgctccc gttcccgctc gagcgcctcc tccagctcca cgcaccgcgc cgcgacgtcg
    10501 tggggcgacg ccgtcatcgg cacggtgatc cgggcccgca cccgctgccg caggtaatcg
    10561 tggagctccc gttccagctc gaggtgcgcc tcggccggtc cggccagcac gagatggtcg
    10621 aacggtcggg ccttgaaggt ccggaacacc gtgtcggcca ccgccttcag gtgcttctgc
    10681 cgccggtcgt ccacgtggcg ctgcaggcgc atctgcgccc accctccttg gtcgtgccgg
    10741 ccggggacct cgccctccac cgcggccacc tcgcggatcc tcccgagctc gcatacgaac
    10801 aaccgggccc gggcgtggtc caccaggacg gcgcagatcg gatggtaggt gtcgagcacc
    10861 tgctcgaggg gcaccaggtc ggcccgaggg ccggccacgg ctcggtcgcg gaccggccgg
    10921 ggcacccgca cctcctccca cagccccgct ccgcgcgagg agaacagcgc cagccctcgc
    10981 gtgtcgccgc gctcgaactc ctcccgcacg taggaggaga tggcgccgag gtcctgctcg
    11041 atccaccgca gggcttccgg gcccaacacc tcggagcggg accgggcctg gcggagcagc
    11101 tcgtccagcc gtacctcgta cccggacctg cgtgggtagc gacggccgtc gacggtcagg
    11161 tacagggacg tcaccggcag cccggccgaa tcccatcccg ccagcgttct cagcagcgtg
    11221 tggtccaggc tcatcatgcg atctcgatcc tttccaggtc cgatcgccct tcccggtccg
    11281 tcagatcgtc ggcccggacg tcgatcaccg agcggacggc gaacaggagc tccctggccg
    11341 cgaggatcag gtgctcgagc gcctcggggt tgctccggtt cacggcggtc agcgccatcc
    11401 cgatcgggca gaaggccacc gggcagacgt gctcctcggg ctggccggcg gccggctcgg
    11461 gcggtggttc cgcggcccgg gaccggccgc cggtggatcg tctcgccctc gtcctggttt
    11521 cagccatgcg tcctctcctt ccgcccaccc cccgaggggt caggcgcgag ctgcctgacg
    11581 aacaggacct ccaggcggtc ctccacgaag ttcgcccccg ccacctccag acgctggagg
    11641 ctctgcggga ggatcaggtt ccgtttgtac gagcccaccc gcacgaacag ctcgtccccc
    11701 cgccggtgga tgtccatgtc gtcccgggag acgaacggca ggcgcatcga cagggcgtag
    11761 ccgccggccc ggcgccggac gcggatcggg tcgtctcgat gcaagacgtc ggtggcgggt
    11821 cgctcgccgt agatctcggc acccatgtcg tcgagcaggt cgacccctac catctcccgg
    11881 tcgaacagcc gggcggtgag gatcgggacg ggctcgaagg actcccgcac cgtctgcagg
    11941 tgctcggcct ggatgtcctt ccacttcccg aagtaggggt cggtgacgtc ctcggggatg
    12001 atccggttca cgatgatcgc gtccacgcgg tacccgaaca gcccgaggta cgtgtaggtc
    12061 cggcgagcct ccgcgatcac catcttctcg gggttcacca ccagacgcac ggacgagctc
    12121 cgttcgtccg tgaggatccg gtgcacgcct tccaggttgc ggtgcaagcg ctcgacggcc
    12181 gtgtacacgc gatcaccggc gatcggcagc gaggtcatcc ggctcatcat gggccgcacg
    12241 gccttcgtca cgcgacgttg caccgggaag atccgctcga tgtaccagtt catgatctcg
    12301 ggcagcgaga gcaggcgcag cgtctcggcg gtgggagcgc agtccaccac caacaggtcg
    12361 tacctcgcat cgtccacgtg gcgcttgacg tcgatcaggc tgaagatctc atccagcccc
    12421 gggatcaccg agagttcttc ggcctggatc gtctcgaccc ccgcccagtt catgagctgg
    12481 accatgtact cctggatctc gcgccagttg gtctccaacc gggcctgcgc atcgatctgc
    12541 tcggcccaca gcaggggcgc caccggggtg ggagcatcga acacctcggt gtcgaaggag
    12601 tcggccaggg agtgagcggg gtcggtgctc atcaccaagg tcctgttccc ggaccgcgcc
    12661 gcccgaacgg cggtggccgc cgcggcggtc gtcttgccca caccgccctt cccggtgaac
    12721 aggaggactc gcatctgggg ctagcttatc cgagccgcgc aggcgagcgg tgggtcagcc
    12781 gagctcgacg cgccgcttca ggccctcgag cgcgttctcg atcacgcgct tcgcgccctg
    12841 ggtccggacg aaccccggca gcaggacgcc gaggtcgacg ctcagccggt aggtgacccg
    12901 cgtgtgccga gcgtcgagct catccaggag gtactcgccc gagatgttgc tcaccgcgcc
    12961 tcgggcctcc ttcgtgaccc acgacagtcc ggcatcgccg ggcgcgtacc ggtaggccaa
    13021 cgtgtagctg gcgcgtccca gcaccgggac gtcgacctcg aaggccacct ccgtcccgag
    13081 gccccccgct ccccgctgca ccacgcgggc ggacttcacg tcggcccact ccggataggc
    13141 ctcgtagtcg gtgatgacgt ccatcacctc ggcggccgag gccccgatga cgatcgagcc
    13201 ctccgcgtgc tcccccatgg cgacggaatc ctaccggccg ctacccaccc cgcacggcgg
    13261 gcagggtgac caggctcgcc agcagcaccg agacaacgat caggaccacg gagccccaca
    13321 gcagcgtccg ggaggagacc accggatcgg cgccggcggc gatccggtag gcgtcgagga
    13381 ccgcgctcat ccagaccgcc accgacgagg cgaggaacag cgcgaacagc ggcagcgtgg
    13441 cccccaggcc ccccttgccg atccgggaca ggatgaggat caggagcgtg ccgaacgtcc
    13501 acgcgaagat cacggcccgg gccacaccgt ccagcgttcg gccgcaacgc caatggccca
    13561 acccagggaa cagcgcggac cacagcgccg ccgtgccggg ctccacctcc ggcgggcgct
    13621 cctgttcctg gaacaagcgg gcgaaggggg tcccgcagac ctcgcacgcg tccaggtgga
    13681 tcgggttctg gtgctggcag accgggcagg gccagcgtgc gtcggcggcg ctcgcgccgg
    13741 ccccggtcgg gaacgcctca tcctggggcg cgtggggagg ctgaggctgg ccgggcatcg
    13801 gcggcggggt cccggccatg gacggcgggg cctcggcggc ggcccgcccg ccccacgtgg
    13861 ccgctcgagg gtcggtggcc tcccgggagg cgggagccgc ctcccgccgg gcgggagagg
    13921 gctccacgag cgacgcgtag cactgtccgc accactcggc atcctcggcc acgagggcgc
    13981 cacacctcgg gcaccggcgg tcggccacca tgccgggagc ctaccgccac atcggggccg
    14041 caccgaacag ccggccgatc cgatcgtccc cacgaccgtg gtcgtcaacc cgccgcggcc
    14101 gcgagccagc cggacagctg ggcggccgcc cggggccagg tgtaggagcg ctccacccgt
    14161 cggcgccccg cctcccccat cctgcgcgcc agccccggat cggccaggag ggcgccgacc
    14221 gcgtccgcca cctccgccac acgggtccca tccaccagga ccccggtggt cccgtccacc
    14281 agcgactcac gtgccccccc cgagtccccc accaccacgg gacggccgca cgcggccgcc
    14341 tcgatgaaga cgttgcccca cccctcgacc tccagaccgc ccaggcgggt ccggcacggc
    14401 atcgcgaaca cgtcccccgc cgcgtagtag cgggg
//
LOCUS       DASQQQ010000312         6798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_299613, whole genome shotgun sequence.
ACCESSION   DASQQQ010000312 DASQQQ010000000
VERSION     DASQQQ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6798
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299613"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VE646_11745"
     CDS             complement(<1..449)
                     /locus_tag="VE646_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80696.1"
                     /translation="MRAKGIFRMGTVRRGGSLLAVLVTASALLLLPLEAVGRADISVR
                     WSRSAGAELASVASGDGAIYVTGSRMKTDGNTAMLLVRYDPSGAVTWTRSWGPRRART
                     DGTSVAVGPDGSVFVVGNVSAENMEGGGWFIRRYSSNGALLWHRTSS"
     gene            complement(672..2186)
                     /locus_tag="VE646_11750"
     CDS             complement(672..2186)
                     /locus_tag="VE646_11750"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZD80697.1"
                     /translation="MDVDRAMGTEVAGYAIESVLGRGAMGVVYVARQASPERRVALKL
                     ITPAMADDRSFRDRFLREARAAAAIDHPHILPVYAAGETDGVLFIAMRLVEGEDLRTI
                     LHAGGPLEPERALSIVWQIGSALDAAHARGLIHRDVKPGNILVAPRSEDDDEDFCYLA
                     DFGVSTWATSSAGTLTSTGHMVGSLHYAAPEQIEGRRAGPAADVYSLGCVLFECLTGH
                     PPFAGRTAPGILYAHLHEDPPAASPLRPELPSGVDPVLARALAKDPEERYGSCRGLGR
                     DLHVTLAGEDVERPASGPVRAPRPVPRRRDRWLGGAALVATALVAAVLVALFVRGDAG
                     PRGGGSPKGSPPPTGAVERIRRGVQVTASLTAPASTDAAGHPVTFLPKNVIDGNVETA
                     WRAPGDGHGVTLTLLFDNPVDVVRIGLIPGYAKTDPETGVNRFQQNRIIRRVRYVIPG
                     LPPIEQTFRPQPVPQFVRVDATTGRITVEILDTGPAHGADDTAISEIYVYGSPQ"
     gene            complement(2224..2871)
                     /locus_tag="VE646_11755"
     CDS             complement(2224..2871)
                     /locus_tag="VE646_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZD80698.1"
                     /translation="MRAGPLTGPWQGAMGEHLEMWVEGARERIPLQGERITIGQTASN
                     DVPLPFDRTVSRVHAVLERVGGRWCVRDLGSRNGTYLNGDRIWGERPLHRGDEIRVGT
                     VRLMAQLDPADPEEDATIAADTGPYLTRREREVLLALCRPMVSGEVFRQPASIRQMAA
                     ELVVTEAAIKQHLVRLYDKFEITQREGRRAQLANEAIRRGAVSTAEILRAETRED"
     gene            2983..3741
                     /locus_tag="VE646_11760"
     CDS             2983..3741
                     /locus_tag="VE646_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80699.1"
                     /translation="MQATPHVLTTGREPRGRRWTRGIAMVLVMILALGATYLWQRAEV
                     HDRQVALDRAAATADAVSVRASGLEATTEQLRGAVERLQGRVQSLTGRLQDVGASKQH
                     LAAQLDALRAETTALLGTPLADGRYFGSLIAVGATQDPPRLVIDLEQFFSGDAADQAA
                     REDGVLPPGQEHVDNDVYIRNESPQWRTLAVDPSATVALTSYPFGQVDGPLMVSIERF
                     GHLLQTGRGSLIWFPYWITVRGAEVVAIEQQFIP"
     gene            3837..4355
                     /locus_tag="VE646_11765"
     CDS             3837..4355
                     /locus_tag="VE646_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80700.1"
                     /translation="MGHLMTPFGAVLRGMVAGAVGTVAMDGVRYARQPQPEQAPGFVA
                     WEKGEQVSGWEDAPAFAQVGKRTAEGLLQRELPDSDARLMRNAVHWGTGVPYGAAYGI
                     LAGSLRKPRPIYGVLLGLAVWALPHVALAPTGLYEPISDLDRQELQTDLGAHLAYGLA
                     TALVFRLLAGGR"
     gene            complement(4359..5000)
                     /locus_tag="VE646_11770"
     CDS             complement(4359..5000)
                     /locus_tag="VE646_11770"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZD80701.1"
                     /translation="MSRREPLLPDAGGGPDFFETAYQGSPTWDIGRPQPAVVRLEQAG
                     RIGGSVLDVGCGTGENALYLASRGHTVVGIDRVERALVRARHKARRRGLAVRFLRQDA
                     LRLDRIGRTFDAAIDVGLFHTLEDGERPVYAAALREVCAPDGRAYLLCWSERNPWGFG
                     PRRVTRREIRETFTGGWRVEALEPEMLDTKLEKGRVHAWLARLAPLARPEPDG"
     gene            complement(5006..>6798)
                     /locus_tag="VE646_11775"
     CDS             complement(5006..>6798)
                     /locus_tag="VE646_11775"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZD80702.1"
                     /translation="WGPVRLRSARVLILLTLACGLAALAVAFRSGLVAAGVAGLVVGG
                     AGAGRELRRITRPDPAPVLEAMARDLGAEALELIRRAYHPGRSGDLQLVMAPGSTANY
                     AQESRALLPRDPRSSHALVWNYLERVPIAVYAPGIVRPSDHVERVSLADLAPTIAHLM
                     SFDGLHPADGVPLPGIQRPATPPKVIVTFVIDGGGWNVLHHWPNAWPNLKRLMRGGAN
                     YRNAIMGSFPAVTACAHATIGTGAFPRTHGITGHNVRKGPRPVKAYGEPGKADPSFIL
                     VPTLADRWAEHTDNAAWQGEIGYQIWHLGMLGAGGRPLGHEPVAVYYDERKTGSWQPQ
                     NPDLYRMPRQVPPVSVLERHQRDYADPHVDDQYESFARGLRSVCCSPPIIQYQGDLIE
                     ATLESEGVGHHDATDLLFTNYKAPDYTGHVYNMLSLREKFALQATDQQLGRLAHYLES
                     HFAPGEFALIVTADHGQCPTVETLGGVRVDPIQLQEDLDREFGGSVFHAVTSVVPSEI
                     YLNHKALWDGGVSRSDLAAFLHEYRYRDNIGPYIKHDAVDWGNLDERPFAAVLSTDFI
                     ATLADADLSRYGEGIFSSHLADPGVPPITW"
BASE COUNT          959 a         2405 c         2459 g          975 t
ORIGIN      
        1 tcactcgagg ttcggtgcca cagcagcgct ccgttcgagg agtaccggcg gatgaaccag
       61 ccgcctccct ccatgttctc ggcgctcacg ttgccgacca cgaagacgga gccgtcgggc
      121 ccgaccgcca cgctggtgcc gtcggtgcgc gcccgccgag gaccccagga gcgcgtccag
      181 gtcaccgccc cgctggggtc gtaacggacc agcaacatgg cggtgttgcc atcggtcttc
      241 atccgggacc cggtcacgta gatcgcaccg tccccggatg ccaccgacgc aagctccgcg
      301 ccggccgagc ggctccaccg caccgagatg tcggcacggc cgacggcctc caacggtagg
      361 agcaggagcg cggacgcggt gaccagcacc gcgagcaggc tgccgccccg ccggaccgtg
      421 cccatccgga agatcccctt ggcccgcatg atgcctcctt ccctccgccg gcccaccgtc
      481 ggcccaccgt cggccctggt ccttggacgg cggacgttgg gacaccggac gccgtggcca
      541 tcccgagttg cgtacgggac gatcgtgcct ccgatggcct gggtggcacc atgccccggc
      601 cggtgcctct ccggttgcct cgagggaggc ttccgtcacc tcctggctac aggtccggcc
      661 gatgcgaagg gctactgcgg ggagccgtac acgtagatct cggagatcgc cgtgtcgtcg
      721 gccccgtggg cgggtccggt gtcgaggatc tccacggtga tccggccggt ggtggcgtcc
      781 acacggacga actggggcac cggctgggga cggaaggtct gctcgatcgg cggcaacccg
      841 gggatcacgt accggacccg ccggatgatc cggttctgct ggaagcggtt caccccggtc
      901 tcgggatcgg tcttcgcgta acccgggatc agcccgatcc gcacgacgtc gaccgggttg
      961 tcgaacagca aggtcagggt gaccccgtgt ccgtcgcccg gcgcgcgcca ggccgtctcc
     1021 acgttgccgt cgatcacgtt cttcggcagg aaggtcaccg ggtggccggc ggcgtccgtc
     1081 gatgccgggg ccgtgaggct cgccgtgacc tgcacgcccc tgcggatccg ctcgaccgca
     1141 ccggtcggcg gaggtgatcc cttcggcgaa ccgccgcccc gaggaccggc atcgcctcgg
     1201 acgaagaggg ccacgagaac ggcggcgacc agcgccgtcg cgacgagggc ggctccaccg
     1261 agccaccgat ccctcctcct cggaacggga cgaggcgcgc gcaccggacc actcgccgga
     1321 cgctcgacgt cctcgccggc gagcgtcacg tgcaggtccc gcccgagccc ccggcaagat
     1381 ccgtaccgct cctccgggtc cttcgccagg gcgcgagcca gcaccggatc gacgccggac
     1441 ggcagctcgg ggcggagggg gctcgcggcg ggcgggtcct cgtgcaggtg cgcgtagagg
     1501 atcccgggag cggttcggcc ggcgaagggc gggtgcccgg tcaggcactc gaacaggacg
     1561 cacccgagcg agtagacgtc ggcggccggc cccgcgcggc gcccctcgat ctgctcgggc
     1621 gccgcgtagt ggagcgagcc caccatgtgt ccggtagatg tcagcgtgcc ggccgatgag
     1681 gtggcccagg tgctcacgcc gaagtcggcg aggtagcaga agtcctcgtc atcgtcctcg
     1741 gatcgggggg cgaccaggat gttgccgggc ttcacgtccc ggtggatcag gccccgagcg
     1801 tgcgcagcgt ccagcgcgga gccgatctgc cagacgatcg atagcgcgcg ctccggctcc
     1861 agcggtccac cggcgtggag gatcgtacgc aggtcctcgc cctccaccag gcgcatcgcg
     1921 atgaacagca cgccgtcggt ctcgcccgcc gcgtacacgg ggaggatgtg ggggtggtcg
     1981 atggccgcgg ccgcccgggc ctcgcgcagg aaccggtcac ggaagctccg gtcgtccgcc
     2041 atggccgggg tgatgagctt gagggctacc cggcgctccg gcgaggcctg gcgggccacg
     2101 tacaccacgc ccatcgcccc gcggcccagg accgactcga tcgcgtaccc ggcgacctcc
     2161 gtccccatcg ctcggtctac gtccatgtcc tcgtcctcgc cggcggctcg gtcgggaccc
     2221 gcgtcagtcc tcgcgggtct cggcccgcag gatctccgcg gtgctcacgg cgccgcggcg
     2281 gatcgcctcg ttggcgagct gggcgcggcg cccctcacgt tgggtgatct cgaacttgtc
     2341 gtacaagcgc accaggtgct gcttgatcgc ggcctccgtg acgaccagct cggccgccat
     2401 ctgccggatg gaggcgggct ggcggaagac ctcgcccgag accatagggc ggcacagcgc
     2461 caggagcacc tcacgctctc gccgggtgag gtacgggccc gtgtcggcgg cgatcgtggc
     2521 gtcctcctcc gggtctgccg ggtcaagctg cgccatcagc cggaccgtgc ccacccggat
     2581 ctcgtcgccg cggtggaggg ggcgctcacc ccagatccgg tccccgttca ggtacgtgcc
     2641 gttgcgcgat ccgaggtcgc ggacgcacca ccgtccgccc acccgctcca gcacggcgtg
     2701 gacgcgcgag accgtccggt cgaacggcag ggggacgtcg ttcgaggccg tctgtccgat
     2761 cgtgatccgt tctccctgga gcgggatccg ctcccgggcg ccctccaccc acatctctag
     2821 gtgctcgccc atggccccct gccacgggcc ggtcagcggc cctgccctca tggtgacata
     2881 tccctcccca tcgacaacag aagcatgcgt gcatagggga tcgtctggac cgcgctgccg
     2941 agcgacgctc tcctcgtcgg atcgatgacg aggaggagag ccatgcaggc cacgccgcac
     3001 gttctgacga ccggtcgcga accacgagga cgacggtgga cccgagggat cgccatggtc
     3061 ctggtgatga tcctcgcgct gggcgcgacc tacctctggc agcgcgccga ggtgcacgat
     3121 cgacaggtcg ccctggaccg ggcggcggcc acggcggacg cggtcagcgt ccgcgcctct
     3181 gggttggagg ccacgaccga gcagctgcgg ggggccgtgg agcggctgca gggaagggtc
     3241 cagagcctca ccggccggct gcaggacgtc ggggccagca agcagcacct cgccgctcag
     3301 ctcgatgcgc tccgagcgga gaccaccgcg ctcttgggca ccccgctggc ggacggccgc
     3361 tacttcggct ccctgatcgc cgtgggggcc acccaggatc caccccggct ggtgatcgac
     3421 ctggagcagt tcttcagcgg ggatgcggcc gaccaggcgg ctcgcgagga cggcgtcctg
     3481 ccgcccggcc aggagcacgt cgacaacgac gtctacatcc gcaacgagag cccccagtgg
     3541 cgcacgctgg cggtcgatcc ctcggccacc gtggcgctca cgtcctaccc cttcgggcag
     3601 gtcgacgggc cgctgatggt gtcgatcgag cgtttcggcc atctgctgca gacgggccgc
     3661 ggctcgctga tctggttccc gtactggatc acggtccggg gggccgaggt cgtcgcgatc
     3721 gagcagcagt tcatcccgtg agtcgacgct gcccggcgct ccccgcgatg tgtgggggcc
     3781 atccggggag gggaccatcc gaagcagggg attggctcgg atggaggagg tcggacatgg
     3841 gacacctaat gacgccgttc ggtgccgtgc tcaggggcat ggtggcggga gcggtcggca
     3901 cggtcgcgat ggacggcgtg cggtacgctc ggcaaccgca gccggagcag gcacccgggt
     3961 tcgtggcctg ggagaagggt gagcaggtga gcggatggga ggatgcgccg gcgttcgcgc
     4021 aggtgggcaa gcgcacggcc gaggggctgc tccagcgcga gctgcccgac agcgacgccc
     4081 gcctgatgcg gaacgccgtg cactggggga ccggcgtccc gtacggcgcc gcgtacggca
     4141 tcctggccgg gtcgctccgc aagccccgtc cgatctacgg tgtgctactg ggcctcgccg
     4201 tgtgggccct gccgcacgtg gcgctggcgc cgacggggct ctacgagccc atctcggatc
     4261 tggatcggca ggagctgcag acggacctgg gagcacacct cgcgtacggg ctcgccaccg
     4321 cgttggtgtt ccgactcttg gccgggggcc gctgaggctc acccgtcggg ctcgggccga
     4381 gccaggggcg cgagcctggc gagccaggcg tgcacccgac ccttttcgag cttggtgtcc
     4441 agcatctcgg gctcgagggc ctcgacccgc cagccgccgg tgaaggtctc ccggatctct
     4501 cggcgcgtga cccggcgcgg gccgaacccc cacgggtttc gctcgctcca gcagagcagg
     4561 tacgcccgtc cgtccggcgc gcagacctct cggagcgcgg cggcgtacac ggggcgctcg
     4621 ccgtcctcca gcgtgtggaa caggcccacg tcgatcgccg cgtcgaaggt ccgtccgatc
     4681 cggtccaacc ggagcgcatc ctggcggagg aaccgcaccg ccagtcctcg gcgccgagcc
     4741 ttgtgacgag ctcggaccag cgcgcgctcc acccggtcga tccccacgac cgtatgtcca
     4801 cgagaggcga ggtacagggc gttctcgccc gtcccgcagc ccacgtcgag gaccgagccg
     4861 ccgatcctgc ccgcctgctc caggcgcacc accgcgggct gcggtcgacc gatgtcccag
     4921 gtcgggctcc cctggtaggc cgtctcgaag aagtcggggc cgccccccgc gtccgggagc
     4981 agcggttccc gccggctcat gggccttacc aggtgatggg cgggacgccc gggtccgcca
     5041 ggtgcgagga gaagatgccc tcgccgtacc gggacaggtc ggcatcggcc agcgtggcga
     5101 tgaagtcggt ggacaggacg gcggcgaaag gccgctcgtc gaggttcccc cagtcgacgg
     5161 cgtcgtgctt gatgtagggt ccgatgttgt cccggtagcg gtactcgtgc aggaacgcgg
     5221 ccaggtcgct ccggctcacg ccgccgtccc acagcgcctt gtggttcagg tagatctccg
     5281 agggcaccac gctggtgacc gcgtggaaca cggagccgcc gaactcgcga tccaggtcct
     5341 cctgcagctg gatcgggtcc acccggaccc cgccgagggt ctccaccgtg gggcactgcc
     5401 cgtggtcggc ggtgacgatc agcgcgaact cgccgggggc gaagtgcgac tcgaggtagt
     5461 gggccagccg ccccagctgc tggtcggtgg cctgcagcgc gaacttctcc cgcagggaga
     5521 gcatgttgta cacgtgcccg gtgtagtcgg gggccttgta gttggtgaac agcaggtccg
     5581 tcgcatcgtg gtgcccgacc ccttccgact ccagcgtggc ctcgatcaga tcgccctggt
     5641 actggatgat cggcggtgag cagcacaccg accgcaaccc ccgggcgaac gactcgtact
     5701 ggtcgtcgac gtgcggatcc gcgtagtcgc gctggtgccg ctcgagcacg gagaccgggg
     5761 ggacctgcct cggcatccgg tacagatccg ggttctgggg ctgccagctt cccgtcttgc
     5821 gctcgtcgta gtagacggcc accggctcgt ggcctaacgg ccggcctccc gccccgagca
     5881 tcccgaggtg ccagatctga tagccgatct cgccctgcca ggccgcgttg tccgtgtgct
     5941 cggcccaccg gtcggccagg gtggggacca ggatgaagga gggatccgcc ttccccggct
     6001 cgccgtaggc cttcaccgga cggggtccct tgcgcacgtt gtggccggtg atcccgtggg
     6061 tccgggggaa cgcgccggtg ccgatcgtgg cgtgggcgca ggcggtcacg gccgggaacg
     6121 agcccatgat cgcgttccgg tagttggcgc cgccccgcat caaccgcttc aggttcggcc
     6181 aggcgttggg ccagtggtgg agcacgttcc agccgccgcc gtcgatcacg aaggtcacga
     6241 tgaccttcgg gggcgtggca gggcgctgga tgccggggag cgggacgccg tcggcggggt
     6301 ggagaccgtc gaaggacatc aggtgagcga tggtcggtgc gaggtcggcc agggacacgc
     6361 gctctacgtg gtccgaaggc cggacgatcc cgggtgcgta caccgcaatc gggacccgct
     6421 ccaggtagtt ccagacgagc gcgtggctcg agcgtggatc gcgcgggagc agggcacgag
     6481 actcctgggc gtagttggcg gtggaccccg gcgccatcac cagctggagg tcgccggagc
     6541 ggccgggatg gtaggcgcga cggatcagct ccagggcctc ggcccccagg tcgcgggcca
     6601 tggcctcgag gaccggagcg ggatcgggtc gggtgatccg ccgcagctca cgcccggccc
     6661 ccgccccacc gaccaccagg ccggccacgc cggccgccac cagcccgctc cggaaggcca
     6721 cggccagcgc ggccaggccg caggcgagcg tcaggaggat caggacgcgg gctgaacgaa
     6781 ggcgcacggg accccatc
//
LOCUS       DASQQQ010000313        12692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_299785, whole genome shotgun sequence.
ACCESSION   DASQQQ010000313 DASQQQ010000000
VERSION     DASQQQ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12692
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299785"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VE646_11780"
     CDS             complement(<1..724)
                     /locus_tag="VE646_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80703.1"
                     /translation="MTGPIPPGAVAVLERGSFCHLAAVTASGPHLTPMVFAWSGHRLW
                     VTTSRRSVKAAVWRRERSVAGLVQAGRTAVSFGGVATTYDALDVGTWERSVLRSPALA
                     RAAVRFSRKNARFFAGYAVDAHRVPMSWTPPGRVFVEIELRRGILLAEDGAEDRWGRW
                     RRSSVSRPSFRADRRGTSPIEALPEPVREALGQAGRGAMAFGADGGSLVLPADWALDE
                     TALYASVRASTLELAGLEDPSPP"
     gene            complement(721..1329)
                     /locus_tag="VE646_11785"
     CDS             complement(721..1329)
                     /locus_tag="VE646_11785"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HZD80704.1"
                     /translation="MVVGLLAEMVAWGTVARRRRSVWPVMAATLAAMGVAAALVGSPP
                     LSTSVPAARAVAVGAGSGVVLYLATRVFVMVVHGWRRFQRHGSRMYARQAGLPLWAAL
                     VLSLAVMVVGEELFWRGLFQLRLGRGLDSHLAGAVLSWLAFVAANLPSANLAIVAGAV
                     VGGAVWTGLAWWSHGFLASMTSHVMWTGLMLSAPVLAAQEGA"
     gene            complement(1348..2124)
                     /locus_tag="VE646_11790"
     CDS             complement(1348..2124)
                     /locus_tag="VE646_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80705.1"
                     /translation="MKGPAGVVSLVLGELVAGGAILVFASPLWGEVKRGFFKVSGVIL
                     FMLSAAAWASARAGGVPGSQAGRWSARLAAAFAVVTLLWVVLLFARQHRAARATGIVS
                     GPVAVAMLAAMAGTAEGSPVVGFLQLLAGAAFLGAVMDGLLLGHWYLTDRGLSRRPIN
                     RYTTFLIVAVVLEAAAVIAGGFGPSEASARFNPLLTSVGLAPWIALGMVGTTALIAVL
                     IRAALRGARSSAVQAATGFFYLAVITALTAELAAKVRFLP"
     gene            2193..2993
                     /locus_tag="VE646_11795"
     CDS             2193..2993
                     /locus_tag="VE646_11795"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZD80706.1"
                     /translation="MEDWRTYDGVAATYERIHAQRVMEPARDLVALADPPNGGRVLDV
                     GTGTGVAAEAARERINPGGLVVGADISLPMLEQGRRARPSVRLVVAEVIDLPFPDGAF
                     DAVVGNFVVTHFTKYETALFDMIRVLRPGGRLALSAWADGVDDLTKTWLEIVESVVGP
                     GMLQDVRSQAAPWAERFRDRSFIEEVLLGAGLKHVRTEQREYRFQYALDEYVEGLQTW
                     TSGRFVRSMLGEGGWERLHAQAREVFADRFSDPVNDFRDVWLAVGTKT"
     gene            complement(3001..3690)
                     /locus_tag="VE646_11800"
     CDS             complement(3001..3690)
                     /locus_tag="VE646_11800"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HZD80707.1"
                     /translation="MLALFAHPDDAEISSGATMAKWAASGREVHLLVLTNGDRGSQDP
                     ALDRAELARIRAGETASAARVMGLVDARVLSTPDGELENTPEVREEVVRAIRTIRPTT
                     LLSCDPTAWFFDNRYYNHADHRTAGSIALDAVFPGAGNPHYFAEHLAEGLSAHDVTDV
                     WLGWALEPNHHEDVTGFMDAKLRALAEHRSQVEGDMLGYFEEWLPKEAEEAGARIGVE
                     HAESFRVLDLS"
     gene            complement(3724..5229)
                     /locus_tag="VE646_11805"
     CDS             complement(3724..5229)
                     /locus_tag="VE646_11805"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HZD80708.1"
                     /translation="MFSYSTDPPADARADLLVLPVLEGPEAGPGIREVKGADLLSLYT
                     DAKLRGKRGEHLLIPDTGALGVRAGAVLLVGVGPRSSIDADALRRAIGKVAPQLAKRG
                     SVVTTFPQAARRSHGEAVQATVEGILLGSYRFSRYKSGLDGPVETDALASVTLLGPSR
                     WDSRAMKAAVRRAEVVSDAVAWARDLVNTPAIDCTPDRLAREAQAMAEETGLECTVWG
                     EAELKEGGFGGILGVGAGSVNPPRMIELVYRGGGKTQAPIALTGKGISFDSGGLSIKT
                     TEGMEWMKGDMGGAASILGAMKAIARLKPKVNVVAAIPSAENMPSGSAIRPGDVLHHR
                     GGKTSEVMNTDAEGRLVLADSLAYLTEQQPRVIIDTATLTGACMVALGDELFGVMGND
                     RSLIRDLLAASEAAGEPAWELPLWPTYRRMLDSEVADFKNVGKRYGGAITAGLFLSQF
                     VGDTPWVHLDIAGPAFAEKAQDYCPKGGTGVPVRTLVGYVLTQAGAGTSRR"
     gene            5326..5664
                     /locus_tag="VE646_11810"
     CDS             5326..5664
                     /locus_tag="VE646_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80709.1"
                     /translation="MDERDLRELVHFSEDEPERSTLFESDHLWSEVICLQGAQGVGPI
                     EDPDSDAICTIVAGTVAVQVDRTRTRIPQWGTVLVPAGASLTLRNASEDPSVILLVAA
                     PPPTSRAVTE"
     gene            complement(5612..7033)
                     /gene="cytX"
                     /locus_tag="VE646_11815"
     CDS             complement(5612..7033)
                     /gene="cytX"
                     /locus_tag="VE646_11815"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HZD80710.1"
                     /translation="MASLTERLERVAAREAPSWGIHPVPSDRRTLSGLDLAVLWGDLS
                     VGLLVMVTGALLVPALGLPRALLAITIGSAVGCLPLALVALAGEREGVPTMVLFRPVL
                     GLRGSFLPSALNLLQLVGWTAVEFWAMAKVANVASIDLFGFDAYHGWLVVVALVATAL
                     AAGGPILVVRRWLERFGIYVLVGTAVWITARVLSSADMAALWRAPGQGGLPFWLGVDL
                     VIVMPVSWLPLAADYNRFARSRAGGLAGTYWGYLVGNVWFYALGVLLVLATGATADVF
                     GIGSAIGAVAGGGFLLIALLVGESDNAFADIYSSALSVQNVAQRVPQRALVTAVGAVG
                     LLLALVLSVDRYQVFLLLIGSVFVPLAGVFLADYFVLRRGRYGRERLFEPSGTRWWAI
                     VPWAVGFVLYHWSVPTGPGGWVDAMHTIFATWLHLPFPLLGSRIGASVPSFAVAFLLS
                     LAVLGPATRSRLSTSGVGPPPAR"
     regulatory      complement(7024..7135)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(7155..7598)
                     /locus_tag="VE646_11820"
     CDS             complement(7155..7598)
                     /locus_tag="VE646_11820"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZD80711.1"
                     /translation="MRVAAQAELPVPMPVAWRRLLAWEEQAGWMQDADRVRVLTSARE
                     GVGVFVAVRTRVLSVPLFTEELEVTVWDPPRRLVMAHRGLVHGVGHWRLEPSAGGTAF
                     TWTEVLRLPPPVLGEIALLAYRPFMRRLMRSSVRNLRAEFLRRTG"
     gene            7668..8165
                     /locus_tag="VE646_11825"
     CDS             7668..8165
                     /locus_tag="VE646_11825"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HZD80712.1"
                     /translation="MSWFTFWLILHILAAVVAFGPTFAFPIIGVMARRQPMHAAFATE
                     VTVTVSERFTIPFALSMPITGTALIVAGHIPFWSSDWLVGSIGLYVVAILYSIGVQRP
                     AGLRMIRLLERVPTGDPGVGATAPAGSPPDVAVLGKKLATGGQVLGPLTLGIFVLMIW
                     RPGAG"
     gene            8290..9531
                     /gene="hutI"
                     /locus_tag="VE646_11830"
     CDS             8290..9531
                     /gene="hutI"
                     /locus_tag="VE646_11830"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HZD80713.1"
                     /translation="MPEQPETPSRGALIATTVVTPIGPAPVRGARLDEVRVLHGGSLA
                     WEDGTLVFVGPASELPAGFEAVDHQGCTVVPGFVDCHTHLPFVGWRADEFETRLAGRS
                     YRELHGEGGIYRSARLLAEASDEQVLNFCGPLVREMADHGTTTLELKTGYGLSVEGEL
                     RQARLAHRLAEIAPQRCLVTLLACHAVPQGRDRASWVRAVCEELIPEVAEASLADAVD
                     VYVEDIAFSTGDLQAVAEAATLAGMGLRCHADQLGDSGAAAAAVALGSRSADHLNHLN
                     GQGIEALGAGTTVATVLPASTFVLRETSPPVRRLLEAQAALALATDFNPGTSPVLSMP
                     EAIAMGCTLYGLTPHQALTAATANPAWVLGVADRTGTLEPGKRADFLVLEGDAFAQVP
                     YRPGHDPVLHTFVGGRRVAGR"
     gene            9528..10700
                     /locus_tag="VE646_11835"
     CDS             9528..10700
                     /locus_tag="VE646_11835"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZD80714.1"
                     /translation="MRSRQGTLVGICVALAVGPLLVLVPPAQAQATDAVTLQADRALI
                     PYGSSVQVTGTVSPPSGGESVQIQDQTPAVLTTATTAPDGSFSATLQLQASSTLQASW
                     NGVVSDPVSVRVRAKVSVRLRNVRLFDRAVARGSVDPAPSGTTVHLTLLLWGRIVARR
                     EVTVSSSGAYRARFPISDPGTYRVRAASRPSGLARGTGTAGPVSTPLPSLKVGSRGSF
                     VRALERRLVALHYHLIGVDTTYDARTADAVMAFRKVQGMPRTGTVDAAVWRRLADPRI
                     PKPRFTAKAFHIEVDQTRQVLYTVRGGEVTNVIHVSTGANGATHDGSFRVHRKLAGFS
                     PNGLYYPSYFDGLRAIHGWTEVPSYPASHGCVRVPYWDARWIFRLAVLGTRVFVYH"
     gene            complement(10728..12248)
                     /gene="hutH"
                     /locus_tag="VE646_11840"
     CDS             complement(10728..12248)
                     /gene="hutH"
                     /locus_tag="VE646_11840"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HZD80715.1"
                     /translation="MQVGERLTLEDVVAVARGESVSLPRSAVARIRAAREVVEAEVAA
                     GHTVYGVTTGFGSLANVRIAPEQADALQRRIVRSHATAVGPPLSREETRAMLLLRAHV
                     LALGHSGVRAAIVQRMIDMLNADLLPLVPEQGSLGASGDLAPLASLALPLIGEGEAMA
                     HGQRMAAGDALEEAGLQPLSLGAKEGLALVNGTQGMLAIGILASDRVARLVRTADVAA
                     AMTIEAALGTDRPFDERLQALRPHPGQARSASNLRRLLARSAITASHRDSEHLVQDAY
                     SFRCAPQVHGATREVLGFAEGILETEAGSVSDNPIVLPSSGGILSGGNFHGQPVAVAL
                     DALAAATVSLAGISERRLFRLLSEDRSNGLPAFLVSESGLNSGFMLVQYTAASLVSEC
                     KSLAHPASVDSIPSSAGQEDHVSMGMTSARHAREIVGNAEVVLAIELLAAAQGLDLRR
                     PLAPAEGTASARDEIRRRVPFLEEDRDLGPDIRAVTEAVRSGSVLAAVEGAIGPLD"
     gene            12359..>12692
                     /locus_tag="VE646_11845"
     CDS             12359..>12692
                     /locus_tag="VE646_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HZD80716.1"
                     /translation="MLEPDDGSGAEPRREGKRPGPTEPGPLRVLARGARARCPRCAAG
                     ALFDGWFHIRERCPRCRLRLEREEGGFLGAMTVNYSVTTLSWLILLGVWLAVDLPDVH
                     VLALTVTSL"
BASE COUNT         1907 a         4711 c         4404 g         1670 t
ORIGIN      
        1 ccggtgggga gggatcctcg agcccggcca gttccagggt ggaggcccgg acggaggcgt
       61 acagggccgt ctcgtccagc gcccagtccg ccggcagcac cagcgagcca ccgtcggcgc
      121 cgaaggccat cgcccccctc ccggcctgcc cgagcgcctc ccgcaccggc tcggggagcg
      181 cctcgatcgg gctcgtgcct cgccgatcgg cccggaagga gggcctcgag accgatgagc
      241 gcctccagcg tccccagcgg tcctccgccc cgtcctcggc gagcaggatc cctcttcgca
      301 gctcgatctc cacgaacacc cggcccggtg gggtccacga catcggcacg cggtgggcgt
      361 ccacggcgta gcccgcgaag aagcgcgcgt tcttgcggga gaaccgcacg gcggcccgcg
      421 ccagcgcggg cgatcgcagg acgctgcgct cccaggtacc gacgtcgagc gcgtcgtagg
      481 tcgtggccac gccgccgaag gagacggcgg ttcgaccggc ctggaccaac cccgccaccg
      541 aacgctcgcg ccgccagacc gcggccttga ccgaccgtcg cgaggtggtc acccagagcc
      601 ggtgccccga ccacgcgaag accatcggcg tcaggtgcgg accggaggcg gtgaccgcgg
      661 ccaggtgaca gaacgagccc cgctccagta cggccaccgc ccctggaggg atcggaccgg
      721 tcatgccccc tcctgcgccg ccagcacggg cgccgacagc atcagcccgg tccacatcac
      781 gtgggaggtc atactggcca ggaacccgtg gctccaccag gccaggccgg tccacacggc
      841 cccacccacc acggcaccgg cgacgatggc caggttggcc gagggtaggt tggcggcgac
      901 gaacgccaac caggacagca cggctcccgc gaggtgagaa tccagccctc ggcccaggcg
      961 gagttggaac agcccccgcc agaacagctc ctcccccacc accatcaccg ccagggagag
     1021 gaccagggcg gcccacaggg gcagtccggc ctggcgcgcg tacatccggg atccatggcg
     1081 ctgaaaccgc cgccagccgt gcacgaccat cacgaagact cgtgtagcga ggtacaggac
     1141 cacgccggac ccggcgccca cggccaccgc ccgagccgcc gggacgctcg tcgagagggg
     1201 cggtgacccc acgagcgcag cggccacccc catcgcggcc agggtggcgg ccatcaccgg
     1261 ccacaccgac cggcgcctcc tcgccaccgt cccccatgcc accatctccg ccagcagtcc
     1321 gacgaccatg acggcgggga ccatggatca gggcaggaag cggaccttgg cggcgagctc
     1381 ggcggtgagg gccgtgatca cggccaggta gaagaacccg gtggccgcct gcactgccga
     1441 ggagcgcgcc ccccgcaggg ccgcccggat caggaccgcg atgagggcgg tggtccccac
     1501 catgcccagg gcgatccagg gggccaggcc gaccgaggtg aggagcggat tgaagcgcgc
     1561 ggacgcctcg ctgggtccga agcccccggc gatcaccgcg gcggcctcca ggaccacggc
     1621 cacgatgagg aaggtggtgt atcggttgat gggccgtcgg gagagccctc gatcggtcag
     1681 gtaccaatgg ccgagcagga gcccgtccat cacggcaccc aggaaggccg ctccggccag
     1741 gagctgcagg aaccccacca ccggggagcc ctcggccgta ccggccatgg cggcgagcat
     1801 ggccacggcc acgggccccg agacgatccc ggtcgctcgg gccgcccgat gctgtcgcgc
     1861 gaacagcagc accacccaca gcagcgtcac cacggcgaac gccgcagcca gccgcgccga
     1921 ccagcgtccc gcttggctgc cggggacccc tccggcccgg gccgacgccc aggcggcggc
     1981 cgacagcatg aagaggatca ccccggacac cttgaagaac cctcgcttca cctcgcccca
     2041 gagcgggctg gcgaagacca ggatcgctcc acccgccacc agctcgccga gcacgaggga
     2101 gaccacaccg gccgggccct tcacgcgcca ccacccatac cggcctaagc ctaccggggc
     2161 ggaaccgaca tcagccgata ggctggtcgt tcgtggagga ctggaggacg tacgacggcg
     2221 tcgccgccac ctacgagcgg atccacgccc aacgggtgat ggaaccggcc cgcgacctgg
     2281 tcgcgttggc cgatccaccg aacggcggaa gggtgctcga cgtggggacc ggcaccggcg
     2341 tggcggccga ggccgcaagg gagcggatca atcccggagg gctcgtggtg ggcgccgaca
     2401 tctcgctgcc catgctggag cagggccgcc gtgcccgccc ctcggtgcgg ttggtggtgg
     2461 ccgaggtcat cgacctgccc ttccccgacg gggcgttcga tgccgtcgtg ggcaacttcg
     2521 tcgtcaccca cttcacgaag tacgaaacgg ccctgttcga catgatccgc gtgctccggc
     2581 cgggaggccg gctggccctg tcggcctggg ccgacggggt ggacgacctc accaagacct
     2641 ggctcgagat cgtggagagc gtggtgggtc ccggcatgct gcaggacgtg cggagccagg
     2701 ccgcgccctg ggcggagcgg ttccgcgacc gatcgttcat cgaggaggtg ctgctcgggg
     2761 ccgggctgaa gcacgtccgc accgaacagc gcgagtaccg gttccagtac gcgctggacg
     2821 agtacgtgga agggctgcag acctggacga gcgggcggtt cgtccggtcg atgctgggtg
     2881 agggggggtg ggagcgtctg cacgcccagg cgcgcgaggt cttcgccgac cgcttctccg
     2941 atccggtgaa cgacttccgc gacgtctggc tggcggtggg cacgaagacc tgaacccatc
     3001 tcacgacagg tcgagcaccc ggaaggactc ggcgtgctcc acgccgatcc tcgcgccggc
     3061 ctcctccgcc tccttcggga gccactcctc gaagtacccg agcatgtccc cctccacctg
     3121 gctgcgatgc tcggccaggg cgcggagctt cgcgtccatg aacccggtga cgtcttcgtg
     3181 gtggttcggc tccagggccc accccagcca gacgtcggtc acgtcgtgcg cgctcagccc
     3241 ttccgccagg tgctcggcga agtagtgcgg gttcccggcc ccggggaaca ccgcgtccag
     3301 ggcgatggag ccggccgtgc ggtggtcggc atggttgtag taacggttgt cgaagaacca
     3361 ggcggtgggg tcgcaggaga gcagggtggt tgggcggatc gtgcggatcg cacgcaccac
     3421 ctcctcgcgc acctcggggg tgttctccaa ctcgccgtcg ggcgtggaca ggacccgggc
     3481 atcgaccagg cccatcaccc gggccgccga ggccgtctcc ccggctcgga tccgggcgag
     3541 ctcggcccgg tccagagcgg ggtcctggga tccacgatcg ccgttggtga ggaccagcag
     3601 gtgcacctca cgtcccgagg cggcccactt cgccatggtg gcacccgagg agatctcggc
     3661 gtcgtcgggg tgtgcgaaga gggccagcac cgccccatca gaaggaaccg cgtccatcgc
     3721 cccctaccgg cgggaggtcc cagcgccggc ctgagtcagc acgtagccga ccagcgtgcg
     3781 gaccgggacg ccggtccccc ccttggggca gtagtcctgc gccttctccg cgaacgccgg
     3841 tccggcgatg tccaggtgga cccacggggt atcacccacg aactgggaga ggaacaggcc
     3901 tgccgtgatg gctccgccgt accgcttgcc cacgttcttg aagtcggcga cctccgaatc
     3961 gagcatccga cggtaggtcg gccagagcgg cagctcccag gccggctcgc cggccgcctc
     4021 gctcgccgcg agcaggtctc ggatcagcga ccggtcgttg cccatcacgc cgaagagctc
     4081 gtcccccagc gccaccatgc acgccccggt gagcgtggcg gtgtcgatga tcactcgggg
     4141 ctgctgttcc gtcaggtagg cgagggaatc ggccaggacc agccgccctt ccgcgtcggt
     4201 gttcatgacc tcggaggtct tcccgccgcg gtgatggagg acgtcgcccg gacggatggc
     4261 cgagccgctc ggcatgttct cggccgaagg gatcgcagcc accacgttca ccttcggctt
     4321 gagccgggcg atcgccttca tcgcccccag gatcgacgcc gcgccgccca tatcgccctt
     4381 catccactcc atgccctcgg tcgtcttgat cgacagaccg cccgagtcga acgagatgcc
     4441 cttgccggtg agcgcgatcg gcgcctgggt cttgccgccg ccgcggtaca cgagttcgat
     4501 catcctcggt gggttcacgc tgccggcacc cacgccgagg atgccgccga accccccctc
     4561 cttgagctcg gcctcgcccc agaccgtaca ctcgaggccc gtctcctccg ccatcgcctg
     4621 cgcctcgcgg gccaagcggt ccggggtgca atcgatggcc ggggtgttca cgaggtcccg
     4681 ggcccaggcc accgcatcgc tcaccacctc ggcccgccgg acggcggcct tcatggcccg
     4741 gctgtcccag cggctcgggc ccagcagcgt cacggaggcc aacgcgtcgg tctccacggg
     4801 tccgtccagg ccggacttgt agcggctgaa ccggtacgag ccgagcagga tgccctccac
     4861 ggtggcctgg acggcctccc cgtgggatcg tcgcgccgcc tgtgggaacg tggtgaccac
     4921 ggacccgcgc ttggcgagct gcggagccac cttcccgatc gcccggcgga gcgcatcggc
     4981 gtcgatcgac gacctcgggc ccacccccac caggaggacc gcgccggccc ggaccccgag
     5041 ggcgccggtg tcggggatca gcagatgctc accccgcttc ccacggagct tggcgtccgt
     5101 gtacaggctc aggaggtcgg ctcccttcac ctcccggatc ccgggaccgg cctcgggccc
     5161 ttccaacacg ggcaggacca gcagatcggc gcgggcgtcg gcgggcgggt cggtcgagta
     5221 ggagaacatg accacggtcg gactccttgg ggcgcttgac cgaggaccag tctatccgcg
     5281 cgcgagcgga caccgagggc ccacgtcgct agcctgagat cggtcgtgga cgagcgggac
     5341 ctgcgcgagc tggtgcactt ctccgaggat gagccggagc ggtcgacctt gttcgagtcc
     5401 gatcacctgt ggtcggaggt gatctgcctc cagggcgccc agggtgtggg ccccatcgag
     5461 gacccggact cggacgcgat ctgcaccatc gtggccggca cggtcgcggt ccaggtggac
     5521 cggacccgga cgaggatccc ccagtggggg acggtcctgg tgccggcggg cgcctcactc
     5581 accctgcgca acgcctcgga agacccgtcc gtcatcttgc tggtggcggc cccacccccg
     5641 acgtcgagag ccgtgaccga gtagccggac ccaggaccgc caacgagagc aggaaggcca
     5701 ccgcgaagct cgggacgctg gccccgatcc tcgacccgag gagcgggaag ggcaggtgga
     5761 gccaggtcgc gaagatcgta tgcatcgcat ccacccaccc cccgggaccg gtgggcacgc
     5821 tccagtggta caggacaaac ccgaccgccc aagggacgat ggcccaccac cgggtcccgg
     5881 agggctcgaa gagccgttcc cgtccgtagc ggccccggcg caggacgaag tagtcggcca
     5941 ggaacacccc cgccagcggg acgaagaccg agccgatcag cagcaggaac acctggtacc
     6001 ggtccaccga gagcaccagg gccagcagga gccccaccgc gcccaccgcc gtgaccaggg
     6061 cccgctgcgg cacccgctgc gccacgttct gcaccgacaa ggcggaggag tagatgtcgg
     6121 cgaacgcgtt gtcgctctcc cccaccaaca gggcgatcag caggaagcca cccccggcca
     6181 ccgccccgat ggccgagccg atgccgaaca cgtcggccgt ggccccggtc gccaggacca
     6241 ggagcacgcc gagcgcgtag aaccacacgt tgcccacgag gtagccccag tacgtgcccg
     6301 ccaagcctcc ggctcggctc cgcgcgaagc ggttgtagtc ggcggccagc gggagccacg
     6361 agaccggcat cacgatcacg aggtccacgc ccagccagaa gggcagccct ccctggcccg
     6421 gagctcgcca cagggcggcc atatcggcgg aggacagcac cctggccgtg atccacaccg
     6481 ccgtgcccac caggacgtag atgccgaacc gctccagcca gcgccgaacc accaggatgg
     6541 gaccacccgc agccagggcc gtcgccacca gggcgaccac caccagccag ccgtgatagg
     6601 cgtcgaaccc gaacaggtcg atggacgcca cgttggcgac cttggccatg gcccagaact
     6661 cgacggccgt ccatcccacc agctgcagca ggttcagggc cgagggcagg aaggagcccc
     6721 gcagccccag gaccggacgg aacagcacca tggtcgggac gccctcgcgc tctccggcca
     6781 gcgcgaccag cgccagcggg aggcacccca ccgcgctccc gatcgtgatg gcgagcagcg
     6841 cccgcggcag ccccagcgcg gggacgagca gggctccggt gaccatcacc agcagcccaa
     6901 cggacagatc gccccacagc accgccaggt cgagcccgga gagcgtccgc cgatccgacg
     6961 ggaccgggtg gatgccccag gacggggcct cgcgggccgc cacccgctcc aagcgctcgg
     7021 tgaggctcgc catctctcgc tccctccgcc ggtacgaacc ggatcaggtt ccaagggtcg
     7081 ccggcttcga gtcggcctct cagcccggtg ccaccgggct cccctgctcg agccagtcta
     7141 cgctggccgg cgcgctatcc ggtccggcgc aggaactcgg cccgcaggtt gcgcacgctg
     7201 ctccgcatca gccggcgcat gaaggggcgg tacgcgagga gggcgatctc gcccagcacc
     7261 ggcggcggca accgcaggac ctcggtccag gtgaacgcgg tgcctccggc ggagggctcc
     7321 agccgccagt ggcccacgcc gtggacgaga ccgcggtggg ccatcaccag acgccggggt
     7381 gggtcccaga ccgtcacctc cagctcctcg gtgaacagcg ggacgctcag cacccgggtc
     7441 cggaccgcga cgaagacgcc caccccttcc cgggccgagg tcaggacccg gacccggtcc
     7501 gcatcctgca tccatccggc ctgctcctcc catgccagca gccgtcgcca ggcgacgggc
     7561 atcggtaccg gcagctcggc ctgcgccgca accctcatcg ggatcctcct tgacgctacg
     7621 cccctcgccc gggagactcg ggccacccct cgacaggggg agcgtccgtg tcctggttca
     7681 cgttctggtt gatcctgcac atcctggcag cggtggtcgc cttcgggccc accttcgcct
     7741 tcccgatcat cggcgtgatg gcccggcgcc agccgatgca cgcggcgttc gcgaccgagg
     7801 tgaccgtcac ggtctccgag cgcttcacga tcccgttcgc gctcagcatg ccgatcaccg
     7861 gcacggccct gatcgtcgcc ggccatatcc ccttctggtc ctccgattgg ctggtggggt
     7921 cgatcggcct gtacgtcgtg gccatcctgt actccatcgg ggtccagcgt ccggcaggac
     7981 tgcggatgat ccgcctcctg gagcgtgtgc cgaccggcga tcccggggtg ggggcaaccg
     8041 cgccggccgg atcccctccc gacgtcgcgg tcctggggaa gaagctcgcc acgggggggc
     8101 aggtcctcgg cccgttgacg ctcgggatct tcgtgctgat gatctggcgg ccgggcgccg
     8161 gctgagcggg gcgccgccga accgcccccc agggtcgcag agcttccggg gcttcatcgg
     8221 gtggtcgacc cagcccctca tcggcccacc acttgcgctg ctgcccacgc tcggattcca
     8281 ttacggggca tgcccgagca gcccgagacg cccagccgcg gcgcgctgat cgcgaccacg
     8341 gtcgtcacgc cgatcggccc cgcccccgtc cgaggcgccc gactcgacga ggttcgggtc
     8401 ctgcacgggg ggtcgctggc gtgggaggac ggaacgctcg tcttcgtggg tcccgcctcg
     8461 gagctgccgg cgggcttcga agcggtcgac caccagggat gcaccgtggt gcctgggttc
     8521 gtggattgcc acacccatct gccgttcgtg ggctggaggg ccgacgagtt cgagacccgg
     8581 ttggcggggc gctcctaccg cgaactgcac ggtgagggcg gcatctaccg gagcgcccgc
     8641 ctgctggccg aggcctccga cgagcaggtc ctgaacttct gcggacccct ggtccgcgag
     8701 atggccgacc acgggaccac gaccctcgag ctcaagaccg gctacggcct gtccgtggag
     8761 ggggagctgc gtcaggcgag gttggcgcac cgactggccg agatcgcccc acagcggtgc
     8821 ctcgtcaccc tcctggcctg ccatgccgtg ccccagggac gagaccgggc ctcctgggtc
     8881 cgcgccgtgt gcgaggaact gatcccggag gtggccgagg catcgctcgc cgatgccgtg
     8941 gatgtctacg tggaggacat cgccttctcg accggggatc tccaggcggt ggcggaggcc
     9001 gcgacgctgg ccgggatggg actgcggtgt cacgccgatc agctcgggga ctccggggcc
     9061 gcagcggcgg ccgtggccct cggctcccgc agcgccgacc acctgaacca cctcaacggg
     9121 caggggatcg aggccctcgg ggccggcacc accgtggcga cggtcctgcc cgcgtccacc
     9181 ttcgtccttc gcgaaacgtc gccgccggtc cgccgtctgc tcgaggcgca ggcggccctc
     9241 gccctggcca ccgacttcaa cccggggacc tcgcccgtcc tgtcgatgcc cgaggcgatc
     9301 gcgatggggt gcacgctgta cgggctgacc ccgcaccagg cgctgactgc cgcgacggcc
     9361 aacccagcat gggtgctcgg ggtggcggat cggacgggta cccttgagcc cggcaagcgg
     9421 gccgacttcc tggtgctcga gggcgacgcc ttcgcccagg tgccctaccg gccaggtcac
     9481 gatccggtgc tgcacacgtt cgttggagga cggcgggtgg cgggacgatg agatccaggc
     9541 agggaaccct cgtcgggatc tgcgtcgctc tcgcggtcgg tccgctgctg gtgctggttc
     9601 cgcccgccca ggcccaggcc acggacgcgg tgaccctcca ggccgaccgt gccctgatcc
     9661 cctacggctc ctccgtccag gtcaccggaa cggtgagccc accgagcggc ggagagtcgg
     9721 tccagatcca ggaccagaca cctgcggtgc tgaccaccgc caccaccgct ccggatggct
     9781 cgttctcggc cacgctccag ctgcaggcct cctcgaccct gcaggcctcc tggaacggcg
     9841 tggtcagcga cccggtgtcg gtgcgggtcc gagccaaggt ctcggtgcgg ctccgcaacg
     9901 tccgtctctt cgaccgggcg gtggcccggg ggagcgtcga tccggcgccg tcgggaacca
     9961 cggtgcacct gacgctcctg ctctgggggc ggatcgtcgc ccgtcgggag gtcaccgtgt
    10021 cgtcgtcggg cgcctaccgg gcccggttcc cgatctcgga tcccgggacc taccgggtgc
    10081 gagcggcctc caggccctcc ggcctggcga ggggcacggg gaccgccggg ccggtttcca
    10141 ccccgctgcc ctcgctgaag gtcggctcga ggggatcgtt cgtgcgagcc ctggagagac
    10201 ggctggtggc tttgcactac cacctgatcg gcgtggacac cacgtacgat gctcggacgg
    10261 ccgacgccgt gatggccttc cggaaggtcc aggggatgcc ccgcacgggg accgtggacg
    10321 ccgccgtgtg gcggcgcctc gcggatccgc ggatcccgaa gccgaggttc accgccaagg
    10381 ccttccatat cgaggtcgac cagacccgcc aggtcctcta cacggtccga gggggcgagg
    10441 tcacgaacgt catccacgtc tccacgggcg cgaacggcgc gacccacgac gggtccttcc
    10501 gcgtccaccg caagctggcc ggcttcagcc cgaacggcct gtactacccc agctacttcg
    10561 acggcctccg ggcgatccac ggatggaccg aggtgccctc ttacccggcc agccatggct
    10621 gcgtccgggt cccctattgg gacgccaggt ggatcttccg cctggccgtg ctcgggaccc
    10681 gcgtgttcgt gtaccactga gtcccgcgcg ggccgcgcgg accggcgtca gtcgagcggg
    10741 ccgatcgctc cttccacggc cgccagcacc gatccgctcc ggacggcttc ggtgacggcg
    10801 cggatgtccg gccccaggtc ccggtcctcc tccaggaacg gcacccgccg gcggatctca
    10861 tcccgggccg acgccgtgcc ctcggccggg gccagcgggc gccgaaggtc cagtccctgg
    10921 gccgcagcga gcagctcgat ggccagcacg acctcggcgt tgccgacgat ctcacgggca
    10981 tgccgggcgg aggtcatgcc catgctgacg tggtcctcct gccccgccga ggaagggatc
    11041 gagtccaccg aggccgggtg ggccagggac ttgcactcgg agaccagcga ggccgccgtg
    11101 tactggacca gcatgaagcc ggagttcagg ccgctctcgg acaccaggaa ggcggggagc
    11161 ccgttgctcc ggtcctccga caggagccgg aacagccgac gctcggagat cccggccagc
    11221 gacacggtgg cggcggccag ggcgtcgagc gccacagcca cgggctgccc gtggaagttg
    11281 cccccggaga ggatgcctcc cgacgagggc agcacgatcg ggttgtcgga gaccgacccg
    11341 gcctccgtct ccaggatgcc ctcggcgaag cccaggacct cccgcgtggc gccgtgcacc
    11401 tgcggggcgc agcggaagga gtacgcgtcc tggaccaggt gctcgctgtc ccggtgggag
    11461 gccgtgatgg cgctgcgggc cagcagtcgg cgcaggttcg aggccgaccg cgcctggccc
    11521 ggatgcggtc ggagtgcctg gagccgttcg tcgaaggggc ggtcggtccc cagggcggcc
    11581 tcgatcgtca tcgccgcggc cacgtccgcc gtccggacca gcctggccac ccgatcggag
    11641 gccaggatcc cgatcgccag catgccctgg gtcccgttca cgagcgccag gccctccttg
    11701 gcgccgagcg acaggggctg gaggccggcc tcctccaggg cgtcgccggc cgccatccgc
    11761 tgcccgtggg ccatcgcttc gccctcgccg atcagcggca gggccaggct ggccagcggg
    11821 gcgagatcgc cggaggcccc cagcgacccc tgctcgggca ccaacgggag cagatcggcg
    11881 ttcagcatgt cgatcatccg ttggacgatc gccgcccgaa cccccgagtg ccccagggcc
    11941 aggacgtggg cccggagcag cagcatcgcc cgggtctcct ccctcgacaa gggtggtccc
    12001 accgcggtgg cgtgggagcg gacgatgcgg cgctggagcg catcggcctg ctccggtgcg
    12061 atccggacgt tggctaggct gccgaagccg gtggtcaccc cgtagaccgt gtgtccggcg
    12121 gccacctccg cctccaccac ttccctggcc gcccggatcc gtgccacggc gctccgcggt
    12181 agggataccg actcgccgcg ggccaccgcc accacgtcct ccagcgtgag acgttcaccc
    12241 acctgcaccg gcacggcggg aaggctacgt gccccggtgg gtgggggcaa cccggggtac
    12301 ccttggtgac cacgtcccga ggagaccgag gctgtccgaa ccgaggggac cctacgacat
    12361 gctcgagccc gacgacggga gcggggccga accccggcgc gagggaaagc gccccggacc
    12421 caccgaaccg ggacccctcc gggtcctggc tcggggcgcc cgggcgcgct gtccgcgctg
    12481 cgccgccgga gcgctgttcg acgggtggtt ccacatccgc gagcggtgcc cccgctgccg
    12541 cctgaggctg gagcgagagg agggggggtt cctcggcgcg atgaccgtga actactcggt
    12601 caccaccctc tcgtggctga tcctgctggg cgtgtggctg gccgtggacc ttcccgacgt
    12661 gcacgtcttg gccctcacgg tgacctcgct gc
//
LOCUS       DASQQQ010000314         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_352000, whole genome shotgun sequence.
ACCESSION   DASQQQ010000314 DASQQQ010000000
VERSION     DASQQQ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352000"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VE646_11850"
     CDS             complement(<1..254)
                     /locus_tag="VE646_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZD80717.1"
                     /translation="MDIRILGSLEAGSDGTTTDLGPPKQRAVLAVLLLHAGETVPTDR
                     LIELVWGEHPPRTAAHSVQIYVSELRKAFETVVDAPIILTR"
     gene            complement(363..908)
                     /locus_tag="VE646_11855"
     CDS             complement(363..908)
                     /locus_tag="VE646_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80718.1"
                     /translation="MAFVVMLSGSLAAIDAAPPSVVVSQVDRAQQRIVAWAVDRYRRV
                     GLELPPLEVHFHPSPGACAGNSGLSLDARIDLCTGTTTIAYARATLLHEMAHAWLEQH
                     ATPELRARFLPLLGLSTWSSRDVPWYRRGVEQAAAILAWGLGDGSLRTLAHDDDPGAL
                     TVAFRSLTGRPPLSPEAVATP"
     gene            complement(1159..2349)
                     /gene="rocD"
                     /locus_tag="VE646_11860"
     CDS             complement(1159..2349)
                     /gene="rocD"
                     /locus_tag="VE646_11860"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HZD80719.1"
                     /translation="MDLVERSSAHNYHPLPVVVAEAEGVWVTDVEGKRYLDMLSAYSA
                     LNFGHRHPALVQAAKRQLDRLTLTSRAFHNDRFGPWAAQLAELAGMEMVLPMNTGAEG
                     VETAIKTARKWGYERKGVPEGRAKVVVCEHNFHGRTTTIVGFSSDQESREGFGPFTPG
                     FVVVPYGDADALADALDEDTVGFLVEPIQGEAGVIVPPEGYLRRARALCTERNVLLIA
                     DEIQSGLGRTGTTFACEHEGVVPDMYVLGKALGGGIMPLSAVVASREILGVFTPGTHG
                     STFGGNPLACAIGLEVLAILRTGELQERSAKLGEHMLRTLREADLSAVRETRGRGLWA
                     GIQLTPGAMPARTRCEELMQHGVLAKDTHEATIRLAPPLVISEQDLDWALERVKQVLA
                     GAPR"
     gene            complement(2497..>3323)
                     /locus_tag="VE646_11865"
     CDS             complement(2497..>3323)
                     /locus_tag="VE646_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018788483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HZD80720.1"
                     /translation="GPGDPTMRISGGEVIRASRTPQGPVSVRFREQGGRVEVGAWGPG
                     REWMLDHAPGWCGASAADGFDPPPGPVREAWRRHRGLRIPRTGLAFEALVPIVLEQKV
                     TGREARRAYGRLVRTLGEPAPGPLGLVLPPEPERIATLPYFAFHPFGIERERAEVLRA
                     LGARAARLDAAAELPVEEAKTSIGAVRGIGPWSVAEVARIALGDADAVSVGDFHVPHV
                     VAWALAAEPRATDARMLELLEPFRPHRGLVQLLLEAAGVRAPAFGPRFEPRAIDRI"
BASE COUNT          461 a         1214 c         1199 g          449 t
ORIGIN      
        1 cgggtgagta tgatcggcgc gtcgaccacc gtctcgaagg ccttgcgtag ctctgagacg
       61 tagatctgca ccgagtgcgc ggcggttctc ggagggtgct ccccccagac cagctcgatc
      121 agccggtcgg tggggaccgt ctcgccggcg tgcaggagca gcacagccaa caccgcgcgc
      181 tgcttcggcg gtcccagatc ggtcgtcgtc ccgtcggagc cggcctccag cgatccgagg
      241 atccggatgt ccacgatcgc gcctcggacc cccccctctt gcgtctcagc ctcagacctt
      301 ggggcgaagg ggacatgggg cgaacgaccc cgctcgcgag ggaccggtct cggctcccct
      361 gctcagggcg ttgccacggc ctcggggctt agcggcggac gtccggtgag ggatcggaag
      421 gcgaccgtga gcgctcccgg gtcatcgtcg tgtgccaggg tccggaggga tccatccccg
      481 aggccccagg ccaggatggc cgcggcctgc tcgacgcctc gccggtacca ggggacgtca
      541 cgagagctcc aggtggaaag acccaggagc ggtaggaacc tggccctcaa ctccggggtg
      601 gcgtgctgct ccagccatgc atgagccatc tcgtgcaaga gcgtggcgcg agcgtacgcg
      661 atcgtcgtcg ttccggtgca cagatcgatc cgagcgtcca gggacagccc ggagttgccc
      721 gcgcacgcgc cggggctcgg atggaagtgg acctcgaggg ggggcagctc gagtcccaca
      781 cgccgatacc ggtcgaccgc ccaggcgacg atccgctgct gcgcccgatc gacctgggag
      841 accaccaccg acggcggagc cgcgtcgatc gcggccaggg acccgctgag catcacgacg
      901 aaggccatcc gcttgacggg gggccctcgg cgcctccggc caccggtgca ggttcttgcg
      961 ccccgaacgg ccacgcccgg cctccccgct cttcgccacc ggagacgggc acgcatccgg
     1021 cggctgtgaa gcgagggtgg ccggggccgc tcgccgcagg gtcaagcgtg gctctacggc
     1081 cgctctatac gagtggtgga tggatgcgcg gcccgcagcg gggaccgggc gggcgtcgat
     1141 cactggcgtg atgggcggct agcgcggagc gccggcgagg acctgcttca cgcgctccag
     1201 ggcccagtcc aggtcctgct cggagatcac cagcggcggg gccaggcgga tcgtggcctc
     1261 gtgggtgtcc ttggccagga ccccatgctg catcagctcc tcgcatcggg tccgggccgg
     1321 catggccccg ggcgtgagct ggatccccgc ccacaggccg cggccccggg tctcccgcac
     1381 ggcggacagg tcggcctcgc ggagggttcg gagcatgtgc tccccgagct tggccgagcg
     1441 ctcctgcagc tccccggtcc gcaggatcgc caggacctcg agcccgatcg cgcacgccag
     1501 cgggttcccc ccgaaggtgg acccgtgcgt ccccggcgtg aacacgccca ggatctcccg
     1561 gctggcgacg acggccgaca ggggcatgat gccgccgccg agcgccttgc ccaggacgta
     1621 catgtcgggc accacgccct cgtgctcgca ggcgaaggtg gtgccggtcc ggccaaggcc
     1681 ggactggatc tcgtcggcga tcagcaggac gttccgttcg gtgcagagcg ctcgggcccg
     1741 gcgcaggtac ccctcgggag ggacgatgac ccccgcctcc ccttggatcg gctccaccag
     1801 gaagccgacg gtgtcctcgt ctagggcgtc ggccagcgcg tcggcgtccc cataggggac
     1861 cacgacgaac ccgggggtga aggggccgaa cccctcgcgc gactcctggt ccgaggagaa
     1921 cccaacgatc gtggtggtcc gcccgtggaa gttgtgctcg cagaccacca ccttcgctcg
     1981 accctcgggc acgcccttgc gctcgtagcc ccacttgcgg gccgtcttga tggccgtctc
     2041 gacgccctcg gccccggtgt tcatcggcag gaccatctcc atgccggcaa gctccgcgag
     2101 ctgggcggcc cacgggccga accggtcgtt gtggaaggcc cggctggtga gggtcaggcg
     2161 atcgagctgg cgtttggccg cctggaccag cgccggatgg cgatgaccga agttcagcgc
     2221 cgagtaggcc gagagcatgt ccaggtaccg cttgccctcc acgtcggtca cccagacgcc
     2281 ctcggcctcg gccaccacca ccgggagcgg gtggtagttg tgcgcgctgg agcgctccac
     2341 gaggtcgatc tgctcggcgg tgccgagcgc gctgcctggg tgctcggtgt gggtggacac
     2401 gtgtgaccgc ctcctcatgc cctggcacgg gccgcgcccg cgcgtgtggc catcatacgg
     2461 tcggcccgga ggttccccgg ggccgaccga tacccctcag atccggtcga tggccctggg
     2521 ctcgaacctg ggcccgaagg ccggggcgcg cacgccggcc gcctccagca ggagctggac
     2581 caggccccgg tgcgggcgga acggctcgag cagctccagc atgcgagcgt cggtcgctcg
     2641 aggctcggcg gccagggccc aggccaccac gtggggcacg tggaagtccc cgaccgagac
     2701 ggcgtcggcg tccccgaggg cgatccgggc gacctcggcc acgctccacg gtccgatccc
     2761 gcggaccgca ccgatcgagg tcttcgcctc ctccaccggc agctcggccg cggcgtcaag
     2821 gcgtgcggcc cgggcgccga gcgctcggag cacctcggcg cgctcacgct cgatgccgaa
     2881 ggggtggaac gcgaagtagg ggagggtggc gatccgctcg ggctccggtg gaagcaccag
     2941 tcccagcggg ccgggagcgg gctccccgag ggtgcgcacc agccggccgt aggcacgccg
     3001 ggcctcccgg ccggtgacct tctgctccag gacgatcggc accagggcct cgaacgccag
     3061 cccggtgcgg gggatccgga gcccacgatg ccggcgccag gcctcccgga cgggccccgg
     3121 cggggggtcg aagccatcgg cggcggaggc cccgcaccac ccgggtgcgt ggtcgagcat
     3181 ccactcccgt cccggtcccc aggccccgac ctccacccgg ccaccctgct cccggaaccg
     3241 cacgctcacc ggcccctgtg gtgtccgtga agcgcggatg acctcgcccc ccgagatccg
     3301 catggtcgga tcgccggggc ccc
//
LOCUS       DASQQQ010000315        10060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_133554, whole genome shotgun sequence.
ACCESSION   DASQQQ010000315 DASQQQ010000000
VERSION     DASQQQ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133554"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VE646_11870"
     CDS             complement(<1..740)
                     /locus_tag="VE646_11870"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Acylates the intermediate (KDO)2-lipid IVA to form
                     (KDO)2-(lauroyl)-lipid IVA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HZD80721.1"
                     /translation="MSTDVQDGAATGLREHETRKERIAFWTYRTGEWLAMTLPERVGS
                     RLFSWLGRVAHRVLPGVRDTVRANQARVLGVAPGSEEADLAAREAFRTYARYWYETFR
                     IRVVPDPEIDRRTELRGIEHIDRALAAGRGCIAVVPHMGNWDAAGRWLAMHGYRVAAV
                     AEELRPPRLSRLFLRHREDLGIRIVPLTKGGQAGQRLAALLADNWVVGLVADRDLSGR
                     GVPVEMFGAVRALPAGPALLSLSSRAPLL"
     gene            complement(737..1228)
                     /locus_tag="VE646_11875"
     CDS             complement(737..1228)
                     /locus_tag="VE646_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80722.1"
                     /translation="MPLIAIVTGVAATGAAAWLVLAGRPTPGGALALLAGALLLAGAS
                     RSHTPERALERTMDAIADRLFDAAVLGAAAWTARIDRPAVAAGALVALGGSFLSAYVR
                     ARGASLGYGVEESSATRGLRYGLVGLGLAAGWFGWTVWVLAALMLLATAVRASQVAKE
                     ELV"
     gene            1375..3468
                     /gene="fusA"
                     /locus_tag="VE646_11880"
     CDS             1375..3468
                     /gene="fusA"
                     /locus_tag="VE646_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZD80723.1"
                     /translation="MKTYDASHIRNVLLVGHGGAGKTTLLEAMLYASGAITRMGSVED
                     GNTVSDFEAEEVKKGISVSLSMAPVEWEGVKVNVLDAPGYVDFVGDLRSAIRAADGVL
                     LVVSAVDGVEVQTEVAWELAVEAGLPRAILINKLDRERASFERTLEQLVRSFGTQVAP
                     LEVPIGEEHAFEGVADLLTRKAYRYPSGPAAQEDEWPAEVTGRADPYREKLMEAVAES
                     DDRLIEKYLEQGELPKDEIVTGVKSGFAEARIAPVLCGAASRPIGVDRILRFIVDEFP
                     APDERPPATVLTRSGEEQQRACDPNGPLTAYVFKTISDPYVGHITMFRVLSGSLRPDS
                     PVFNATKGTEERVGQLFTLQGKDHDTVSEVQAGDVGAVAKLSATTTGDTFSTKDDPVT
                     VPPVAMPEPLLAFAIEPKTKGDEDKLSTALARLREEDPTFRVERSDETHETVMYGMGE
                     AHLDVMIERMKRKYGVEVTTAPAKIAYRETIRSSGKAMGRHVKQTGGHGQYAICNIEV
                     EPMERGAGFEFVDKIFGGAIPNQFIGSVEKGVVKAMSEGVLSGNPMVDVRVTLVDGKF
                     HPVDSSDMAFQIAGSLALKEATQDAGVVLLEPIVELEVVVPDDNTGDIMGDLNSKRAK
                     IQGMESAGAGKQRVRALVPQAEVARYAIDLRSLTGGRGAFAMRFSHYEEVPSHLADRL
                     VAESQKAKEEASAKK"
     gene            3522..4763
                     /locus_tag="VE646_11885"
     CDS             3522..4763
                     /locus_tag="VE646_11885"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HZD80724.1"
                     /translation="MGLPPGAEGVLPSQLLRRAAELGVVDAGDYKIPTSSIQPASLDL
                     RLGEVAYRIRSSFLPDKRPVEVKLKEFLVDELDLRRDGAVLETNRPYVVPLIEHLDLP
                     PDVRGKANPKSSTGRLDVFTRVITDQSFRFDEIRPGYRGNVYLEIVPLSFTVRVKEGL
                     ALNQLRLSVGRSRLSDEEIRALHADQTLLFQDERAVADADLAVSDGLFLSLDLRGDAT
                     GRVGYRAKDFAPPLEMAWVGGYGWRDYWEPVQREEGDRIVLAPEHFYLLLSEEAVRIP
                     PDVAAEMTAYDPTSGELRTHYAGFFDPGFGYDPENRFHGSRAALEVRAHDVPFVIEHR
                     QRVCRLSFERMIERPDVLYGTSIGSSYQGQVDTLGKHFKGPGEDAAEELDPASGEGSA
                     RGGAGGQGTAGRSEHPVLFED"
     gene            complement(5273..5659)
                     /locus_tag="VE646_11890"
     CDS             complement(5273..5659)
                     /locus_tag="VE646_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HZD80725.1"
                     /translation="MDRAFLAFGAWFGFFAVAFGAFGSHALSARLAPERIGTFEIGVR
                     YQLYHGLAMFVVVFLRTIGPDTFAETLAGILFIAGVILFSGSLYLLAITGVRRWGAVT
                     PFGGLCLLGGWLLVAWAIASTTFRFA"
     gene            complement(5680..6486)
                     /locus_tag="VE646_11895"
     CDS             complement(5680..6486)
                     /locus_tag="VE646_11895"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZD80726.1"
                     /translation="MSLQGFVAVDGGSLYYERAGMGPGVVLIHPGLWDSRTWDDQFPR
                     FAERFLTVRYDVRGYGRSTRPEPGRVYSHVDDLLAVMEAAGIERAVLVGCSMGGGIAL
                     EAALSHPDRVAALVLAASALPGFEPTPGEEAWWEEHAAGIEELVDAGELEAAVRIEME
                     IWAPLGVEDEAGARILQIALDNRHELTMDEQDARGLDPPAVQRLESIAVPTLVLPADH
                     DPPTSQRASAILAERIPGARLAQIPEVDHVINVRRPDEFDRVVLEFLGQI"
     gene            complement(6483..6968)
                     /locus_tag="VE646_11900"
     CDS             complement(6483..6968)
                     /locus_tag="VE646_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014121409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HZD80727.1"
                     /translation="MDRIWSPWRMRYIESAKDGVDGCIFCDLPERGDDATVHILARAP
                     LSFVMLNTYPYNPGHVMVAPIRHTGDLEALSAEELADVQRLIQVSVAALREEMDPHGY
                     NVGMNLGRVAGAGIPDHLHWHVVPRWNGDTNFMPVIGQTRVLPELLDETYRKLVHRFQ
                     R"
     gene            complement(6968..8887)
                     /gene="thrS"
                     /locus_tag="VE646_11905"
     CDS             complement(6968..8887)
                     /gene="thrS"
                     /locus_tag="VE646_11905"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019642483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HZD80728.1"
                     /translation="MPTIHLPYGRDVELPEGEPIGSVLPPEALAARFDGELVDLTFVP
                     PGSGAAEPVGAGEGDALQVLRHSTAHVMAQAVCGLYPGARYAIGPPVDDGFYYDFDLE
                     APLSPEDLEKIEGRMAEIVAADQPFVREELSRAEALKRFADQPYKREIIEGLDESEGA
                     LGDRVSVYRNEGWSDLCLGPHVPSTGRIGAFKLMKLAGAYWRGDESRQMLTRIYGTAW
                     GSREELEDHLRRLEEAERRDHRRLGRELDLFSSPEELGPGLWIWHPRGGIFRKELEDY
                     ARDLHLERGYDLVVTPHIARSVLWETSGHLAKYMENMYPPMQDEQGDYYVKPMNCPFH
                     VLVYKSRTRSYRDLPMRLSELGTIYRHERIGTLHGLLRIRGGTQDDSHIVCTPEQLVE
                     EILGVFDLTLEILGTFGFTEPIIKLSTLPGQTIVTPEMAARATEALRNALEKSGLPYQ
                     LAEGEGAFYGPKVDFDFRDAIGRPWQLTTVQCDFGLPERFDMEYVGEDNQRHRPVMIH
                     RAILGTIERFSGVLIEHYAGAFPLWLAPEQVWLAPVSQDFLSHARTLATRFQEAGLRA
                     HVDESDETVGKKVRAAQLMKAPYTLVIGKREMESGLLTVRDRTGTETKGVSVDAFVEA
                     LVREASARALEQSRW"
     gene            complement(8899..9021)
                     /locus_tag="VE646_11910"
     CDS             complement(8899..9021)
                     /locus_tag="VE646_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80729.1"
                     /translation="MELSVPVGRLVGYAIPGVVAAAVPARRAARLNVLEAIAYE"
     gene            complement(9026..9300)
                     /locus_tag="VE646_11915"
                     /pseudo
     CDS             complement(9026..9300)
                     /locus_tag="VE646_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015802797.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(9335..9886)
                     /locus_tag="VE646_11920"
     CDS             complement(9335..9886)
                     /locus_tag="VE646_11920"
                     /inference="COORDINATES: protein motif:HMM:NF022101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HZD80730.1"
                     /translation="MSNQGPPMDPNTEEATPEQIAAAKAQGAAYGAALELMADVVAET
                     GGQREAGDYLVGYAIEEAEGMYEPVDGSLEWREPDDENVHVEISVRDRGDGRFVPGLT
                     VMATLIDPDGNQVGTHQQPMLWHPMLYHYGRNWTVPGDGEYTLRVRIEPAPFMRHDKV
                     NGRRFSEPVECEFTGVQVETGQD"
BASE COUNT         1545 a         3615 c         3450 g         1450 t
ORIGIN      
        1 agcaaggggg cccgggagga cagcgagagc agagcaggtc cggccggcag ggctcggacc
       61 gccccgaaca tctccaccgg gacccctcgg cccgagaggt cccggtccgc caccagaccc
      121 accacccagt tgtcggcgag cagggccgcc agccgttgac ccgcctggcc ccccttggtg
      181 agcgggacga tgcggatccc gaggtcttcc cgatggcgca ggaacagccg ggacaggcgg
      241 ggggggcgga gctcctcggc caccgcggcc acccgatagc cgtgcatcgc gagccagcgc
      301 cccgcggcgt cccagttgcc catgtgcggg accaccgcga tgcagcccct gccggcggcc
      361 agcgcccggt cgatgtgctc gatgccccgc agctccgtcc gccggtcgat ctccgggtcg
      421 gggaccaccc ggatccggaa cgtctcgtac cagtagcggg cgtacgtccg gaacgcctct
      481 cgggccgcca ggtcggcctc ctcggagccc ggcgccaccc ccagcaccct ggcctggttg
      541 gcccggaccg tgtcccgaac cccgggcagg acccggtggg cgacgcggcc cagccacgag
      601 aacagacggg agccgacccg ttccggcagc gtcatcgcga gccactcgcc ggtgcggtag
      661 gtccagaagg cgatccgctc ctttcgcgtc tcgtgctccc gcagaccggt ggccgccccg
      721 tcctggacgt ccgtgctcac accagctcct ccttcgccac ctggctggct cggacggccg
      781 tggcgagcag catcaacgcg gcgagcaccc acacggtcca gccgaaccac cccgccgcca
      841 gccccaggcc caccaggccg taccggagtc cgcgcgtcgc cgagctctcc tccacgccgt
      901 agccgaggga ggctcctcgg gcccggacgt aggcagagag gaagctccct cccagggcca
      961 cgagcgcgcc cgcggccacc gccggccgat cgatccgcgc ggtccaggcc gccgccccca
     1021 gtaccgccgc gtcgaacagg cggtcggcga tcgcatccat cgtgcgctcc agcgcccgct
     1081 cgggggtgtg ggagcgcgag gcgcccgcca ggagcagggc cccggccagc agggccagcg
     1141 ccccgcccgg cgtgggccgc ccggccaaga cgagccaggc ggccgcgccg gtcgccgcca
     1201 cgccggtcac gatggcgatc agcggcaccc gtcgctcccc ggtccgacct tgcccgacag
     1261 caccgacacg gagcggcgag cgtatcaccg gtcggtgggt cggccgtcgg gggccagcag
     1321 gccgcgcgct agacttccgc tgaaggacgc cggagctcgc tggacgggag atcgatgaag
     1381 acctacgacg cctcgcacat ccgaaacgtc ctgctcgtcg gccacggggg cgcgggcaag
     1441 accacgttgc tggaagccat gctgtacgca tcgggcgcca tcacccggat gggcagcgtc
     1501 gaggacggca acaccgtctc cgacttcgag gccgaagagg tgaagaaggg gatcagcgtc
     1561 tccctgtcca tggccccggt ggagtgggag ggggtcaagg tcaacgtcct ggacgctccc
     1621 gggtacgtgg acttcgtggg ggacctgcgc tcggcgatcc gggctgccga cggggtactc
     1681 ctggtggtct ccgcggtgga tggggtcgag gtccagaccg aggtggcctg ggagctcgcc
     1741 gtggaggccg ggctgcctcg cgcgatcctc atcaacaagc tggaccggga gcgggcgtcg
     1801 ttcgagcgga ccctcgagca gctggtgcgc tcgttcggca cgcaggtcgc cccgctcgag
     1861 gtcccgatcg gcgaagagca cgcgttcgag ggggtcgccg acctgctcac ccgcaaggcc
     1921 taccggtacc cctcgggacc agccgcccag gaggacgagt ggcccgcgga ggtcaccggc
     1981 cgggcggacc cttaccgcga gaagctgatg gaggccgtgg cggagtccga cgaccggctg
     2041 atcgagaagt acctggagca gggcgagctc cccaaggacg agatcgtgac gggggtgaag
     2101 tcgggcttcg ccgaggcccg gatcgcgccg gtgctctgcg gggcggcgtc ccgtccgatc
     2161 ggcgtggacc ggatcctcag gttcatcgtg gacgagttcc ccgccccgga cgaacggccc
     2221 ccggccacgg tgctcacccg ctcgggcgag gagcagcagc gggcctgcga ccccaacgga
     2281 cccctcacgg cctacgtgtt caagacgatc tccgaccctt acgtggggca catcacgatg
     2341 ttccgggtgc tgtcgggctc cctgcgcccc gactcgccgg tgttcaacgc caccaagggc
     2401 accgaggaac gggtgggcca gctgttcacc ctgcagggca aggatcacga cacggtctcc
     2461 gaggtgcagg cgggcgacgt cggggccgtg gccaagctgt ccgccaccac caccggggac
     2521 accttctcca ccaaagacga tccggtcacc gtgccccccg tggcgatgcc cgagcccctg
     2581 ctggccttcg ccatcgagcc caagacgaag ggggacgagg acaagctgtc caccgccctg
     2641 gcccggctcc gcgaggagga ccccacgttc cgcgtggagc gctccgacga gacccacgag
     2701 actgtgatgt acggcatggg cgaggcgcac ctcgacgtga tgatcgagcg catgaagcgc
     2761 aagtacgggg tggaggtcac cacggccccg gccaagatcg cctaccggga gaccatccgg
     2821 tcctcgggca aggccatggg ccggcacgtg aagcagacgg gcggccacgg ccagtacgcg
     2881 atctgcaaca tcgaagtgga gccgatggag cgcggggcgg ggttcgagtt cgtggacaag
     2941 atcttcggcg gcgcgatccc caaccagttc atcggctcgg tcgagaaggg cgtggtgaaa
     3001 gcgatgtccg agggggtgct ctcgggcaac ccgatggtcg acgtgcgggt caccctcgtc
     3061 gacggcaagt tccacccggt ggactcctcc gacatggcct tccagatcgc cgggtccctg
     3121 gcgctgaagg aggccacgca ggacgccggc gtggtactgc tagagccgat cgtcgagctc
     3181 gaggtcgtgg tgcccgacga caacacgggc gacatcatgg gcgatctgaa ctcgaagcga
     3241 gccaagatcc agggtatgga gtcggccggc gccggcaagc agcgggtgcg ggccctggtc
     3301 ccccaggccg aggtagcccg ctacgcgatc gacctgcgat ccctcaccgg cgggcggggg
     3361 gcgttcgcga tgcgcttctc ccactacgag gaggtgccct cccacctggc cgatcgcctc
     3421 gtggccgaga gccagaaggc caaagaagag gcttcggcca agaagtaggg ggggcggacc
     3481 tgagccagga gcagcaccga cccgagccgg gggcatcccg cctggggctc cccccggggg
     3541 ccgagggcgt cctccccagc cagctcctgc gcagggccgc cgagctcggc gtggtcgatg
     3601 ccggcgacta caagatcccc accagcagca tccagcccgc cagcctggac ctgcggctcg
     3661 gggaggtggc ctaccggatc cgctcgagct tcctgccgga caagcggccg gtcgaggtga
     3721 agctgaagga gttcctggtg gatgagctcg atctccggcg ggacggagcc gtcctcgaga
     3781 ccaaccgtcc gtacgtggtg ccgctgatcg agcacctgga cctgcccccg gacgtgcggg
     3841 ggaaggcgaa cccgaagagc tccaccggcc ggctcgacgt gttcacgcgg gtgatcacag
     3901 atcagagctt ccgcttcgac gagatccgcc ccggctacag gggcaacgtg tacctggaga
     3961 tcgtcccctt gtcgttcacg gtccgcgtga aggaagggct ggccctgaac cagctgcggc
     4021 tgtcggtggg acggtcccgg ttgagcgacg aggagatccg ggcgctgcac gctgaccaaa
     4081 cgctgctgtt ccaggatgag cgcgcggtcg ccgacgccga cctggcggtc tccgacggcc
     4141 tgttcctgag cctggatctg cgcggggacg ccaccggccg ggtgggatac cgggccaagg
     4201 acttcgcgcc gcctctggag atggcgtggg tgggcggcta cggatggcgg gactactggg
     4261 agccggtgca gcgagaggag ggcgatcgga tcgttctggc ccccgagcac ttctacctgc
     4321 tgctctccga ggaggccgtc cggatccccc ccgacgtggc ggccgagatg accgcgtacg
     4381 atccgaccag cggcgaactg cggacgcact acgcggggtt cttcgatccg gggttcggct
     4441 acgaccccga gaaccgcttc cacggctccc gggccgccct ggaggtgcgc gcgcacgacg
     4501 tgcccttcgt gatcgaacac cgccagcgcg tgtgccgcct ctccttcgag aggatgatcg
     4561 agcgcccgga cgtcctgtac ggcacctcga tcggctcctc gtaccaaggt caggtggaca
     4621 cgctcggcaa gcacttcaag ggtccgggcg aggatgcggc cgaggagctg gacccggcga
     4681 gcggtgaggg ctcggcgcgt ggtggggcgg gtgggcaggg gacggcgggc cggagcgagc
     4741 acccggtgct gttcgaggac tgagttcgag gtccgagcgc cgtcaccagg agggcttcga
     4801 cagatctcac ccgggtgtga cccacatcac tgcggtgaga tcgactgttc cctacctggt
     4861 actcacggtg gatgccgggg tgctgggcgg gtcagggggt gagggcctcg ctgatgcggg
     4921 tggtgctgtc cgctgggagc ccttggatat gggaaggcgc acccccactg gtgcccccgg
     4981 ccctgcgccg ggcggtgatc gtgcgggata cggggtgtcg cttccccggg tgcgctcggc
     5041 cggcctcctg gtgcgatgcg caccacgtgc cctggaccag gggtggagcg accagcctgg
     5101 ccaacctgct cctgctgtgc cgtcggcacc accgggcgtg caccagcacc gggggttcac
     5161 gctggtgctg gaggacgggc ggccggtgtt ccgaaggccg gacgggtcgg tgctggagga
     5221 ggtgcgtggc cccaccgtag gggtggggcg ggtccgggcg ggcccaacaa gatcaagcga
     5281 atctgaaggt ggtgctcgcg atcgcccagg ccaccagcag ccagccgccc agcaggcaca
     5341 ggccgccgaa gggcgtcacg gccccccagc gtcggacgcc cgtgatcgcc aggaggtaca
     5401 ggctgccgct gaacaggatc acgcccgcga tgaacaggat gccggccagg gtctcggcga
     5461 aggtgtccgg accgatcgtg cgcaggaaga ccaccacgaa catggcgagg ccgtggtaca
     5521 gctgatagcg cacgccgatc tcgaaggtgc cgatccgctc gggggcgagc cgagcgctga
     5581 gggcgtggga cccgaaggcc ccgaacgcga cggcgaagaa cccgaaccac gcgccgaagg
     5641 cgaggaacgc tcgatccatg ggtgaacctc cgaactcgtc tagatctggc ccaggaactc
     5701 cagcacgacc cgatcgaact cgtcgggtct gcgcacgttg atcacgtgat cgacctcggg
     5761 gatctgcgcc agcctggccc cggggatccg ctccgccaag atcgcgctgg cgcgttggga
     5821 ggtcggcgga tcgtggtccg cggggagcac cagcgtggga acggcgatgc tctccaggcg
     5881 ctgcacggcg ggcgggtcga gcccgcgggc gtcctgctcg tccatcgtga gctcgtgacg
     5941 gttgtccagc gcgatctgca ggatcctggc ccctgcttcg tcctccaccc cgagcggcgc
     6001 ccagatctcc atctcgatcc gaaccgccgc ctcgagctca ccggcgtcca cgagctcctc
     6061 gatccccgcc gcgtgctcct cccaccacgc ctcctccccg ggtgtgggct cgaaccccgg
     6121 cagcgccgac gccgccagca ccaacgccgc cacacggtcc ggatgcgaca gggccgcctc
     6181 gagcgcgatc cccccgccca tcgagcagcc caccagcacc gcccgctcga tcccggccgc
     6241 ctccatcacc gcgagcaggt cgtcgacgtg cgagtacacc cggccgggct ccggacgcgt
     6301 ggagcggccg taccctcgga cgtcgtagcg cacggtgagg aaccgctcgg cgaaccgtgg
     6361 gaactggtcg tcccaggtcc tcgagtccca cagcccggga tggatcagca ccacccccgg
     6421 tcccatcccg gcccgctcgt agtacaggct cccgccgtcc acggcgacga agccctggag
     6481 gctcaccgct ggaaccggtg gacgagcttg cggtacgtct cgtccagcag ctcgggcagc
     6541 acgcgcgtct gcccgatcac cggcatgaag ttcgtgtcgc cgttccaccg gggcaccacg
     6601 tgccagtgca ggtggtcggg gatccccgcc cccgccaccc gccccaggtt catgcccacg
     6661 ttgtagccgt gcgggtccat ctcctcgcgc agcgccgcca ccgaaacctg gatcagtcgc
     6721 tgcacgtcgg ccaactcctc cgccgacagg gcctccaggt cgcccgtgtg ccggatcggg
     6781 gccaccatca cgtgcccggg gttgtacggg taggtgttga gcatcacgaa ggaaagcggg
     6841 gcccgagcca ggatgtggac ggtggcgtcg tcgccccgct ccggcagatc gcagaagatg
     6901 cacccgtcca cgccgtcctt ggccgactcg atgtaccgca tccgccaggg cgaccagatg
     6961 cggtccatca ccaccgactc tgttcgagcg cgcgcgccga cgcctcgcgc accagcgcct
     7021 cgacgaaggc atccaccgac acgcccttcg tctcggtgcc ggtgcgatct cggaccgtga
     7081 gcagccccga ctccatctcc cgcttgccga tcaccagcgt gtacggcgcc ttcatcagct
     7141 gcgcggcccg caccttcttg ccgaccgtct catcgctctc gtccacgtgc gcccgcagtc
     7201 cggcctcctg gaaccgggtg gcgagcgtgc gcgcgtggga caggaaatcc tggctcacgg
     7261 gcgcgagcca cacctgctcg ggagccagcc acagcgggaa cgcccccgcg tagtgctcga
     7321 tcagcacgcc actgaaccgc tcgatcgtgc cgaggatcgc gcgatggatc atcaccggcc
     7381 ggtgccgctg gttgtcctcg cccacgtact ccatgtcgaa ccgctcgggc aggccgaagt
     7441 cgcactggac ggtggtgagc tgccacggcc gcccgatcgc gtcgcggaaa tcgaagtcca
     7501 ccttcgggcc gtagaaagcg ccttccccct cggccaactg atagggcaag ccgctcttct
     7561 ccagggcgtt cctgagggcc tcggtggccc tggccgccat ctcgggggtc acgatcgtct
     7621 gcccgggcag ggtggagagc ttgatgatcg gttcggtgaa cccgaaggtc ccgaggatct
     7681 ccagggtcag gtcgaacacg ccgaggatct cctccaccaa ctgctcgggc gtgcagacga
     7741 tgtgggagtc gtcctgcgtt cccccacgga tcctcagcag gccgtggagc gtcccgatgc
     7801 gctcgtgacg gtagatcgtg cccagctcgg acagtcgcat cggcaggtcg cggtacgagc
     7861 gggtccggga cttgtagacc agtacgtgga aggggcagtt catcggcttg acgtagtagt
     7921 cgccctgctc gtcctgcatg ggcggataca tgttctccat gtacttcgcc agatgcccgc
     7981 tggtctccca cagcaccgaa cgcgcgatgt gcggggtgac gaccaggtcg tagccccgct
     8041 ccaggtgcag atcgcgcgcg tagtcctcga gctccttgcg gaagatccct ccacgcgggt
     8101 gccagatcca caggccgggg ccgagctcct cgggactgga gaagagatcg agctcgcgtc
     8161 ccagccggcg gtggtcgcgg cgctcggcct cctccagacg gcgcaggtgg tcctcgagct
     8221 cctcgcgcga cccccacgcc gtgccgtaga tccgggtgag catctgccgg gactcgtccc
     8281 cgcgccagta cgccccggcc agcttcatca gcttgaacgc cccgatccgc ccggtggagg
     8341 ggacgtgcgg gcccaggcag aggtcgctcc agccctcgtt gcggtacacg gagacccgat
     8401 cgccgagcgc cccctcgctc tcgtcgagcc cctcgatgat ctcgcgcttg tacggctgat
     8461 cggcgaagcg cttcagcgcc tcggcgcgcg agagctcctc ccgcacgaac ggctgatcgg
     8521 cggcgacgat ctccgccatc cgtccctcga tcttctccag gtcctcgggc gagaggggcg
     8581 cttccaggtc gaagtcgtag tagaagccgt cgtcgaccgg aggcccgatc gcgtaccgag
     8641 ctcctggata caggccgcac acggcctggg ccatcacgtg cgccgtcgag tgccgcagca
     8701 cctgcagagc gtcgccctcc cccgctccca ccggctccgc cgccccggat cccgggggga
     8761 cgaaggtcag gtcaaccagc tcgccgtcga accgggccgc caacgcctcg gggggcagca
     8821 ccgacccgat gggctctccc tcggggagtt cgacgtcacg gccgtacggg agatggatcg
     8881 tgggcatgga gggccatgct actcgtaggc gatggcctcc agcacgttta gcctggccgc
     8941 ccgccgggcc ggcaccgccg cggccaccac cccggggatc gcgtagccca ccagccgacc
     9001 caccggcacc gacagttcca tcacggttgt aggcctggaa gacgaacccg accttcttcc
     9061 gtcggagagc tgcttctccg acagctcggt caggtccacg tcgccgatca gcacccggcc
     9121 ggaggtggga cgtccagccc cgccaggcag tgcagcatcg gcttgcccga accggaggcg
     9181 cccatgatcg tgctgaactc ggccgccccg aagtccacca gatccccgga gggcctccac
     9241 cgcagcctcc ccggtgccgt agaccttgcg caggtccacg gttcgagccg ccaggcgcat
     9301 cccaccgttc cttcccttcc cggtcggggg ggacctagtc ctgtccggtc tcgacctgca
     9361 cgccggtgaa ctcgcattcc accggctcgg agaagcgccg cccgttcacc ttgtcgtgcc
     9421 gcatgaaggg agccggctcg attcgcactc ggagcgtgta ctccccgtcc cccgggacgg
     9481 tccagttgcg gccgtagtgg tagagcatgg ggtgccacag catcggctgc tggtgggtac
     9541 cgacctggtt cccatcggga tcgatcagcg tggccatcac ggtcagaccc ggcacgaacc
     9601 ggccgtcgcc gcggtcgcgc acgctgatct cgacgtgcac gttctcgtca tcgggctcgc
     9661 gccactccag cgagccatcg accggttcgt acatcccctc ggcctcttcg atcgcgtagc
     9721 ccacgaggta gtcgccggcc tcccgctggc ccccggtctc ggccaccacg tcggccatta
     9781 gctcgagggc cgccccgtag gccgccccct gggccttggc agcggcgatc tgctccgggg
     9841 tcgcttcctc ggtgttcgga tccatcggcg ggccctggtt gctcatggtt gcctccttcg
     9901 agctcctcga ggcctaccta cccgtcgctg cgagccgcca cccctcgtgc gcgtcgcctc
     9961 ggcgccttcc cggttcgctc catgagcccc gggtggtgtc cccaaccggt ctgccctaag
    10021 gtcatcttcg ggcgcatctg atatccgcaa ggtgaagcgt
//
LOCUS       DASQQQ010000316         3059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_50066, whole genome shotgun sequence.
ACCESSION   DASQQQ010000316 DASQQQ010000000
VERSION     DASQQQ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50066"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /gene="dnaA"
                     /locus_tag="VE646_11925"
     CDS             <1..1362
                     /gene="dnaA"
                     /locus_tag="VE646_11925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HZD80731.1"
                     /translation="SATLRAHVPDAAWRTCFQGVTPLQVDGESLVLGIPSSLAKERIE
                     SRYLPLIHRALAEAGNGTLDVRLEIHTDVAPDTALGEPPLLTHHVAGEAVRPHDPPPD
                     PADTPSRVEQMGLNPRYTFDNFVTGASNRFAHAAALSVAETPARSYNPLFIYGSAGLG
                     KTHLLHGIAHYVHQNYPAYKVRYVATEVFMNDFVDAIRTNTTTEFKRRYRESDVLLID
                     DIQFLENKEGLQEEFFHTFNSLHGANRQIVISSDRPPRAIATLEDRLRSRFEWGLITD
                     IQPPDLETRLAILRKKAEREPTPVPPEVLEYIATHITDNIRELEGALIRVAAYASLNR
                     APLTLELAQQVLGAIISAGRPRTITPTIILEETAKMFGFTVDELQGKSRRRPLVTARQ
                     VGMYVCRELTELSYPAIAREFGGRDHTTVIHAVEKVGALMKERRQIYEQVSELIQSIK
                     SGG"
     gene            1607..2701
                     /gene="dnaN"
                     /locus_tag="VE646_11930"
     CDS             1607..2701
                     /gene="dnaN"
                     /locus_tag="VE646_11930"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HZD80732.1"
                     /translation="MKFRCERDALVEALATATRAVAVRGGALPVLSGVRFELAGSRLT
                     LAGSDLDLTIQVTAEVAGDRDGVCVVPARLVADIVRALEPGAVQVEAEGEEARIASGR
                     SQFAVRALPAEDFPRLPDAAGDDVTLEATEFADALRQVVRAASQDDARPILTGVLMAA
                     EGDGLRLVATDSYRLAVRDLPGTSVLREGQQVLVPSKALGELGRVLGGAEQVTLRLGE
                     RDAVFSVGDTRISTRLIEGEFPNYRQLIPSSYPNRLTVGREPLLDAIRRVKLLAREAT
                     PVRLRLGGDGLELSAITQDVGHAEEEIDAKYEGSEMVIAFNPEYLADGVDAIHGDEIS
                     LETVDALKPAVVRTVDGSDYLYLLMPVRVS"
     gene            2708..>3059
                     /locus_tag="VE646_11935"
     CDS             2708..>3059
                     /locus_tag="VE646_11935"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZD80733.1"
                     /translation="MRLARLWLTNFRNYAATDLVPGTGLTVIAGGNGEGKTNLLEAVG
                     WLATLSSFRGALTDALVRAGAEQAVVRAAVAPSEGREVLIEAELHAQGRDRVQVNRQR
                     LRRTRDLLGTFRVTV"
BASE COUNT          487 a         1080 c         1040 g          452 t
ORIGIN      
        1 tccgccactc ttcgtgccca cgtccctgac gccgcctggc gcacctgctt ccagggggtg
       61 acgcccctcc aggtcgacgg ggagagcctg gtcctgggga tcccgagctc gctcgccaag
      121 gagcggatcg aaagccggta cctgcccctc atccaccggg cgctggccga ggccggcaac
      181 ggcacgctgg atgttcgcct cgagatccac accgacgtgg cgcccgacac ggcgctgggt
      241 gagccgccgc tgctcaccca ccacgtggcc ggggaggcgg tccgtccgca cgacccgccg
      301 cccgacccgg ccgacacccc ctcccgggtg gagcagatgg gcctgaaccc ccgctacacg
      361 ttcgacaact tcgtgaccgg ggcgtcgaac cgcttcgccc acgccgccgc cctctcggtg
      421 gccgagaccc cggcccggtc ctacaacccg ctgttcatct acggcagcgc cgggctcggc
      481 aagacccacc tgctgcacgg catcgcccac tacgtgcacc agaactaccc ggcctacaag
      541 gtgcgctacg tcgcgaccga ggtcttcatg aacgacttcg tggacgccat ccgcaccaac
      601 accaccaccg agttcaagcg ccggtaccgg gagagcgacg tcctcctcat cgacgacatc
      661 cagttcctgg agaacaagga aggcctccag gaggagttct tccacacgtt caactcgttg
      721 cacggtgcca accgtcagat cgtgatcagc tccgatcgcc cgccccgggc catcgccacg
      781 ctcgaagacc ggctgcgcag ccggttcgag tgggggctga tcaccgacat ccagccgccc
      841 gatctcgaga ccaggctggc gatcctccgc aagaaggccg agcgggagcc cacgccggtc
      901 ccgcccgagg tgctggagta catcgccacc cacatcaccg acaacatccg cgagctcgag
      961 ggggcgttga tccgggtggc cgcctacgcc agcctcaacc gggcgccgct caccctggag
     1021 ctcgcccagc aggtgctcgg ggccatcatc tcggccggtc gaccccgcac gatcaccccg
     1081 acgatcatcc tcgaagagac cgccaagatg ttcggcttca cggtcgacga gctgcagggc
     1141 aagagccggc gccggccact ggtgaccgcc cgccaggtcg gcatgtacgt gtgccgcgag
     1201 ctcaccgagc tcagctatcc ggcaatcgcc cgggaattcg gtggtcgtga ccacaccacg
     1261 gtcatccacg cggtcgagaa ggtgggcgcc ctcatgaagg agcgtcgtca gatctacgaa
     1321 caggtctcgg agctcatcca gtcgatcaag agcgggggat gacctccacg agctgggagc
     1381 aactctgggg acgtcctgtg ggtgaccatg tgggccgacc tctgttgtcc acaacctcgc
     1441 ccggggttcc gcccatgagg gatcagcctg tggaacgctg tggacagcgc gcggtgggtg
     1501 cgcggtgcga cccaccagcg ggaatggacg tctgtccaca atccacagcc cttattaccg
     1561 ctactagaag atctctctaa gagagacgag aaggagatcg gtcacggtga agttccgctg
     1621 cgagcgagac gctctggtcg aagcgctcgc cacggccacc cgagcggtgg cggtgcgggg
     1681 tggtgctctc ccggtgctgt cgggggtccg cttcgaactg gctgggagcc ggctcacgct
     1741 ggccggaagc gacctggatc tcaccatcca ggtcaccgcc gaggtcgccg gcgatcgcga
     1801 cggggtctgt gtcgtgcccg cccgcctggt ggccgacatc gtccgggctc tggagccggg
     1861 ggcggtgcag gtcgaagccg aaggggagga ggcccggatc gcctcgggcc ggtcgcagtt
     1921 cgcggtacgt gcgctcccgg ccgaggactt cccccggttg cccgacgccg ccggcgatga
     1981 tgtcacgctc gaggccaccg agttcgccga cgccctgcgc caggtcgtgc gggccgccag
     2041 ccaggacgac gcccgcccca tcctcaccgg cgtgctgatg gccgccgagg gagacgggct
     2101 gcggttggtg gccaccgact cctatcggct ggcggtgcgg gatctccccg gtacgagcgt
     2161 gcttcgggag ggccagcagg tgctggtgcc gtccaaggcc ctgggcgagc tcggccgggt
     2221 gctgggcggg gccgagcagg tcaccctgag gctcggcgag cgggacgcgg tgttcagtgt
     2281 gggtgacacc cggatcagca cccggctgat cgaaggcgag ttccccaact accgccagct
     2341 catccccagc tcgtacccga accggctcac ggtcgggcgg gaaccgctgc tcgatgccat
     2401 ccgccgggtg aagctgttgg ctcgggaagc cacgccggta cggctgcggc tgggcggcga
     2461 cggcctcgag ctcagcgcca tcacccagga tgtgggtcac gcggaggagg agatcgacgc
     2521 caagtacgag ggcagcgaga tggtcatcgc cttcaacccc gagtacctgg ccgacggggt
     2581 cgatgccatt cacggtgacg agatcagcct ggagacggtc gacgcgctca agccggcggt
     2641 ggtccgtacg gtggacgggt ccgactacct ctacctgttg atgcccgtgc gggtgtcgtg
     2701 acccgcggtg cggctcgccc gcctctggct cacgaacttc aggaactacg ccgccactga
     2761 tctcgttccc ggcaccggcc tcaccgtgat cgccggcggc aacggcgagg gcaagaccaa
     2821 cctcctcgag gcggtcggtt ggctggcgac cctgtcgtcg ttccgggggg cgctgaccga
     2881 cgcgctcgtt cgggcgggcg cggagcaggc ggtggtgcgg gccgcggtcg ccccctccga
     2941 gggccgcgag gtgctcatcg aggccgagct gcacgcccag ggacgcgacc gcgtgcaggt
     3001 caaccgccag cggctgcggc gcacccgcga tctgctgggc acgttccggg tgacggtgt
//
LOCUS       DASQQQ010000317         2020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_323084, whole genome shotgun sequence.
ACCESSION   DASQQQ010000317 DASQQQ010000000
VERSION     DASQQQ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323084"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1058)
                     /locus_tag="VE646_11940"
     CDS             complement(180..1058)
                     /locus_tag="VE646_11940"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HZD80734.1"
                     /translation="MSAERLLRNQRLRNIVPPRVKAAFAENRVRLVRVSRRSELRNVY
                     HCCVHKTGSQWIRKVLADPDVYRATGLRTYAYAPRLPEERKNLGYSGLRFDTPFPRHT
                     IVSPMYIDLEGFEALPKPEPWRALFVMRDPRDVAVSWYFSSTRSHPTRTNQSLQRTRE
                     QLRALEEEQGLIFTIRRLQDYGLFHALRSWNQAASEDVLVVRYEDLIGDDAGERWDRV
                     LDHCDVRLTPSERRSLLQRYSFERLSGRAPGQEDPGSKLRKGVAGDWRNHFTPAVAEA
                     FVEVTSDLAAEIGYPA"
     gene            complement(1074..>2020)
                     /locus_tag="VE646_11945"
     CDS             complement(1074..>2020)
                     /locus_tag="VE646_11945"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZD80735.1"
                     /translation="VPGQTGGAPAVSAAGPRIDRPVFVIGTGRSGLTPLMDLIAYHRA
                     FGWPSQYNERWPRFPRVSALSRIVEVPPFDRRLKLRRGVPKHTEAYPLWNACFPGFAE
                     PFRDLVAEDATPYAERRFRHAVGQILAHQRKPRFIAEYSGWSRIGFLRAIFPDARFIH
                     IVRDGRAVAHSYTTVDWWNGWEGVYRWRLGLPDPETMGKLSGYGDSFLALAAVYWKIL
                     VRNISEAGRGLPEGDFLQVRYEDLVADPSKEARRCIEFAGLDAEDVRFTRHLRTVRIV
                     DANRTQMRIPPWREAIDERQIAMFADLAGEELAEFHYL"
BASE COUNT          298 a          714 c          723 g          285 t
ORIGIN      
        1 cccgccggat gccgggcgcc ccggtcgagg tgcggcgggc ggagctgtcc ccggacctcc
       61 gccgacggct cgaggaggcg ctcgggcccg acgtcgctcg gctccggcgc ttcatgccgc
      121 agggcttcga cggctggggg atcggctgag ggaacggagg tcgcggcctc cgtgacccgc
      181 tacgcggggt acccgatctc ggcggccagg tccgacgtga cctccacgaa cgcctcggcc
      241 acggcggggg tgaagtggtt gcgccagtcg cccgccaccc ccttgcgcag cttcgagccc
      301 ggatcttcct gacccggggc ccgacccgac agccgctcga agctgtagcg ctgcaggagc
      361 gagcggcgct cggacggcgt gaggcgcacg tcgcagtggt ccagcacccg gtcccatcgc
      421 tcccccgcgt cgtcaccgat caggtcctcg taccggacca ccagcacgtc ctccgaggcg
      481 gcctggttcc agctccggag ggcgtggaag agcccgtagt cctgcagacg acggatcgtg
      541 aagatcagcc cctgctcctc ctccagcgcg cggagctgct cccgggtgcg ctgcaggctc
      601 tggttggtgc gggtgggatg cgaccgggtg gaggagaagt accagctcac cgccacgtcc
      661 cgcggatccc gcatcacgaa cagggcccgc cacggctccg gcttgggcag ggcctcgaag
      721 ccctcgaggt cgatgtacat cggcgagacg atcgtgtggc gcgggaacgg ggtgtcgaag
      781 cggagccccg agtagcccag gttcttccgc tcctcgggca ggcggggagc gtaggcgtag
      841 gtccgcagtc ccgtggcccg gtacacgtcg ggatcggcca ggaccttgcg gatccactgg
      901 ctcccggtct tgtggacgca gcagtggtac acgttgcgga gctccgagcg gcgcgacacg
      961 cggaccaggc gcacccggtt ctcggcgaag gccgccttca cccggggggg cacgatgttg
     1021 cgcaggcgct ggttgcggag cagccgttcg gcggacatga cgtggacccc ccgctagagg
     1081 tagtggaact cggccagctc ctcgccggcc aggtcggcga acatcgcgat ctgtcgctcg
     1141 tcgatggcct cccgccaggg cgggatccgc atctgcgtgc ggttcgcgtc gacgatcctc
     1201 accgtgcgca ggtgacgggt gaaccggacg tcctcggcat ccaggcccgc gaactcgatg
     1261 cagcgccggg cctccttcga ggggtcggcc accaggtcct cgtagcgcac ctgcaggaag
     1321 tcgccctccg ggagcccgcg gccggcctcg gagatgttgc ggaccaggat cttccagtac
     1381 acggcggcca gggccaggaa cgaatccccg tacccggaga gcttgcccat cgtctcaggg
     1441 tcgggcagtc ccagccgcca ccggtacacg ccctcccagc cgttccacca gtcgaccgtg
     1501 gtgtaggagt gcgccaccgc gcgtccgtcc cggacgatgt ggatgaaccg ggcgtcgggg
     1561 aagatcgccc tcaggaaccc gatcctggac catcccgagt actcggcgat gaacctcggc
     1621 ttgcgctgat gcgccaggat ctggccgacg gcgtggcgga accgccgctc cgcgtagggg
     1681 gtggcgtcct cggccaccag atcgcggaag ggctcggcga agcccgggaa gcacgcgttc
     1741 cacagcgggt aggcctcggt gtgcttgggg accccccggc gcaacttcag gcggcgatcg
     1801 aagggcggca cctccacgat gcgggacagg gccgagaccc tcgggaaccg aggccagcgc
     1861 tcgttgtact gcgacggcca gccgaacgcc cgatggtagg cgatcagatc catcagcggg
     1921 gtgagcccgc tccgccccgt gccgatcacg aagaccggcc ggtcgatcct cggtccggcg
     1981 gcgctcaccg caggcgcgcc acccgtctgg cccgggacca
//
LOCUS       DASQQQ010000318         4128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_352447, whole genome shotgun sequence.
ACCESSION   DASQQQ010000318 DASQQQ010000000
VERSION     DASQQQ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352447"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1924)
                     /locus_tag="VE646_11950"
     CDS             complement(<1..1924)
                     /locus_tag="VE646_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HZD80736.1"
                     /translation="MSVSSIGRPVSRALVVVTLAGASFIVPGVAEAAPICGNSGGQTI
                     CVAVPSGTLSGPATITITNSANTGTLIARWIPSGKSAIQLITEFGPSPSTNDYTFVWP
                     TQKYLDDSGVLRVQAGSTSSSPVDVPVSLSNGNTSDFQHAPNDWADFLPDPSWTASRD
                     PVVAAVGDGASNDPEANDNADAIAASNPDLFLYLGDIYEDGTATENLNHYGQSSMDGG
                     PGTLWGQMADITQPAIGDHEVSNRDAWRDYFHGRPLYTSFVFGNVLFLDLASTGESMA
                     VGSPQYDWVKSILTSSSAPACVVSYFQNPALAKDKIKTKNGRADMWALLTDNGGDLVL
                     NGNTHTMLQYKPLNDKLQLPSSGQATQVELIAGSGGHKMGGTFTGDSRVEWSKGKTPG
                     AVYITLNGAADGGSPTSLSWAYKDAKGNVLHTGSRDCGGGSPPPPPPGLSITGFSPTS
                     GPVGTSVQITGTGFTGTSDVSFGGASATSYTVDTDSQITATVPASASTGPISVTTGAG
                     TATSSSAFTVTSSGGGGGLAISGFTPTSGPVGTSVTIAGSGFSGVTVVKFNGVKAHFT
                     VDSDAQITATVPSSATSGPIKVKDPAGAVTSASDFTVSSGGGGGGTLAISGFSPTSGP
                     VGSSVTITGSAFSGVTI"
     gene            complement(2003..3073)
                     /locus_tag="VE646_11955"
     CDS             complement(2003..3073)
                     /locus_tag="VE646_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZD80737.1"
                     /translation="MTFEWSSRRVLVTGGASFIGSTLVDALVRRGARVRIVDDFSSGV
                     MRNVQHVLDEGSAQLIRGDLRDPDVAAGAVAGMDTIFHLAADHGGRGYVDLHQYACST
                     NLGLDATLFGAALRAGVEQIVYASSGCVYPLYLQADPGEILYLTEDMLGAPGYDPDGL
                     YGLAKLAGELTLRALHEESGMKTASCRYFTVYGPRGVENHAVIAMIARAFLRQDPFHV
                     WGNGEQIRNWTYVDDIVEGTILAAEKIDDGTAVNLGTTERVRVLDAVKMVCELAEYSP
                     EIVLQPDMPTGPMNRVADNSLARGLLGWEPQVPFAEGLRRTLDWYFSTKDAAQVATIL
                     EHMLTGRGVPEGEPDLVAASGE"
     gene            3288..>4128
                     /locus_tag="VE646_11960"
     CDS             3288..>4128
                     /locus_tag="VE646_11960"
                     /inference="COORDINATES: protein motif:HMM:NF012449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HZD80738.1"
                     /translation="MRIKILCTLGPASLRADVIRALDRQQIDLFRINLSHTPLEAVEP
                     TIDLVQAHSATPICLDSEGPQVRCGMIDGLGEAQLEAGQTIRLVSEHVPGTSDQVTLW
                     PASAFGALTPGARVGIDFDGVQLRVTTSDGRSAEAVVVSPGRIRSNKAVTIDPKPHLP
                     ALSPKDLEAIGIGARKGIAHYALSFAGSAKDVDRLRSLAPAGTHIIAKIESKQGVRNM
                     DHIIEAADSVLVDRGDLSREIALESVPYYQKAIVRRANRWNRPVYVATNLLESMVTGN
                     VPTI"
BASE COUNT          628 a         1445 c         1407 g          648 t
ORIGIN      
        1 cgatggtcac gccgctgaac gcgctccccg tgatggtcac gctgctgccg accggccccg
       61 acgtgggcga gaacccggag atggccaggg tcccgccccc gcccccgccg ctggagacgg
      121 tgaagtcgga ggcgctggtg accgctccgg cagggtcctt caccttgatc ggcccgctgg
      181 tggccgagct ggggacggtg gccgtgatct gcgcgtcgga gtccacggtg aagtgggcct
      241 tcacgccgtt gaacttcacc acggtgacac cgctgaaccc gctccccgcg atggtcacgc
      301 tggtgccgac cggccccgat gtcggggtga acccggagat cgccagccct cccccgcccc
      361 cggaggaggt gaccgtgaac gcggaggagc tggtggccgt gccggctccc gtcgtgaccg
      421 agatcggccc ggtggaggcc gaggctggca ccgtggcggt gatctggctg tcggtatcca
      481 ccgtgtagct ggtcgcactg gccccgccga agctcacgtc gctggtgccg gtgaacccgg
      541 tcccggtgat ctgcaccgag gtccccacgg gtccggaggt cgggctgaac ccggtgatcg
      601 acagaccggg gggcgggggt ggcggcgagc caccgccgca gtcgcgggag cccgtgtgca
      661 gcacgttgcc cttcgcgtcc ttgtaggccc aggacaggct ggtggggctc ccgccgtcgg
      721 ccgcaccgtt cagggtgatg tacacggctc cgggggtctt gcccttggac cactccaccc
      781 gggagtcgcc ggtgaaggtc ccgcccatct tgtggccgcc cgagccggcg atcagctcca
      841 cctgggtggc ctgcccggac gaggggagct ggagcttatc gttcagcggc ttgtactgga
      901 gcatcgtgtg ggtgttcccg ttcagcacga ggtcgccccc attgtcggtc aggagcgccc
      961 acatgtccgc ccggccattc ttcgtcttga tcttgtcctt ggccagcgcc gggttctgga
     1021 agtaggagac cacgcacgcc ggtgcggagg agctggtgag gatcgacttg acccaatcgt
     1081 actgagggct ccccaccgcc atcgattccc cggtggaggc gaggtccagg aacagcacgt
     1141 tgccgaagac gaacgaggtg tagagcgggc gcccgtggaa gtagtcgcgc caggcatccc
     1201 ggttcgagac ctcgtggtcc ccgatggccg gctgggtgat gtccgccatc tgcccccaca
     1261 gcgtgcccgg gccgccgtcc atcgaactct ggccgtagtg gttgaggttc tcggtggcgg
     1321 tcccgtcctc gtagatgtcc cccaggtaca ggaacaggtc cgggttggag gccgcgatgg
     1381 catccgcgtt gtcgttggcc tcgggatcgt tgctggcgcc gtcgccgacc gcggcgacca
     1441 cggggtccct ggaggcggtc cacgacgggt ccggcaggaa gtccgcccag tcgttcggcg
     1501 cgtgctggaa atcactcgtg ttgccgttgg acaggctcac cgggacgtcc accggcgagc
     1561 tcgaggtcga cccggcctgg acccgcagca cgcccgagtc gtccaggtac ttctgggtgg
     1621 gccacacgaa cgtgtagtcg ttggtgctcg gcgagggccc gaactcggtg atcagctgga
     1681 tcgccgactt gccgctgggg atccaccgag cgatgagcgt ccccgtgttg gcgctgttgg
     1741 tgatggtgat ggtggcgggg cccgacagcg tgccgctcgg caccgcgacg cagatcgtct
     1801 gcccgcccga gttgccgcag atcggcgccg cctccgcaac cccagggacg atgaacgaag
     1861 ccccggccag cgtcaccacc accagggcac gagacacggg acggccgatc gacgacacgc
     1921 tcatggtgcc acctacctcc cccttcacca ccgccgagcg atggagatgc agccgggcgc
     1981 gccgtgcgcc ggcgccggtc cgtcactcgc cggatgccgc caccaggtcc ggctcgcctt
     2041 cgggcacgcc ccgaccggtc agcatgtgct ccaggatggt ggccacctgg gcggcgtcct
     2101 tggtcgagaa gtaccagtcc agcgtccggc gcagtccctc ggcgaagggg acctggggct
     2161 cccagcccag caggccacgg gccagcgagt tgtcggccac ccggttcatc ggaccggtcg
     2221 gcatgtcggg ctgcaggacg atctccgggc tgtactcggc cagctcgcag accatcttca
     2281 cggcatcgag cacacggacc cgctcggtgg tcccgaggtt gacggccgtc ccgtcgtcga
     2341 tcttctcagc cgccaggatc gtgccctcca cgatgtcgtc cacgtaggtc cagttccgga
     2401 tctgctcgcc gttgccccac acgtggaagg ggtcctggcg caggaacgcc ctggcgatca
     2461 tggcgatcac ggcgtggttc tccacgcccc gaggtccgta cacggtgaag taccggcacg
     2521 aggccgtctt catcccggac tcttcgtgca gcgcgcggag ggtgagctcc cccgccagct
     2581 tggccagtcc gtacagaccg tccgggtcgt accccggggc cccgagcatg tcctccgtga
     2641 ggtagaggat ctcaccggga tccgcctgca agtagagggg gtacacgcag ccggaggacg
     2701 catagacgat ctgctccaca ccggcccgaa gcgcggctcc gaacagggtc gcgtcgagcc
     2761 cgaggttcgt ggagcacgcg tactggtgca ggtccacgta cccccggccg ccgtggtccg
     2821 ccgccaggtg gaagatcgtg tccatcccgg ccaccgcacc ggcggcgacg tcggggtccc
     2881 gcaggtcgcc tcggatcagc tgggcggagc cctcgtccag cacgtgctgg acgttgcgca
     2941 tcacgccgct ggagaagtcg tccacgatcc ggacccttgc cccccgtcgg accaacgcat
     3001 ccaccagggt ggagccgatg aaggaggcac ctcccgtcac cagcacccgc ctcgaagacc
     3061 actcgaacgt cacgcagctc gcctcctcat cgcatcatcg tcacccgaac catccggctc
     3121 ggtcgccgca ggagatggac ggttcccgcc tgagggaaca gggtacccat ccgggcgccc
     3181 ctgcattgtc cgagcgccaa ccgagatgcg tcttcgcgcg attgcatgat tcgaggtaca
     3241 tccgatggac gacgagaacc ggccctgacc tccctacaat cgccgtcatg aggatcaaga
     3301 tcctgtgtac gcttggccct gcgtcgctcc gcgccgacgt gatccgggcg ctggaccgcc
     3361 agcagatcga cctgttccgg atcaacctgt cccacacgcc tctcgaggcg gtggagccga
     3421 cgatcgacct ggtccaggcc cactcggcca cgccgatctg cctggacagc gaggggcccc
     3481 aggtgcgctg cggcatgatc gacgggctgg gggaggcgca gctcgaggcg gggcagacca
     3541 tccggctggt ctccgagcac gttccgggca cgagcgacca ggtcacgctc tggccggcgt
     3601 ccgcgttcgg ggccctcacc cccggggcac gggtgggcat cgacttcgac ggtgtgcagc
     3661 ttcgggtcac caccagcgac ggtcgctcgg cggaggccgt ggtggtctcg ccgggccgga
     3721 tccgctcgaa caaggcggtc acgatcgatc ccaagcccca cctgcccgcc ctcagtccga
     3781 aggacctgga agcgatcggg atcggcgccc gcaagggcat cgcccactac gccctctcgt
     3841 tcgcgggatc ggcgaaggac gtcgaccggt tgcgctcact ggccccggcc ggcacccaca
     3901 tcatcgccaa gatcgagagc aagcagggcg tgaggaacat ggaccacatc atcgaggcgg
     3961 ccgactccgt gctggtcgac cggggcgacc tgtcccggga gatcgccctg gagagcgtgc
     4021 cctactacca gaaggcgatc gtgcgtcgcg cgaaccgctg gaaccggccc gtgtacgtcg
     4081 ccacgaacct cctggagtcc atggtcaccg gcaacgtgcc gacgatcg
//
LOCUS       DASQQQ010000319         2322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_50198, whole genome shotgun sequence.
ACCESSION   DASQQQ010000319 DASQQQ010000000
VERSION     DASQQQ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50198"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VE646_11965"
     CDS             complement(<1..833)
                     /locus_tag="VE646_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZD80739.1"
                     /translation="MPIRRTEFGTGLRVVTERMPGVRSVALGFWVLAGSRDEPPRISG
                     SSHFLEHLLFKGTRSRTARDIAEAFDAVGGDVNAFTAKEYTCFYARVQDRDLPMAVEH
                     LCDMLQHSVIRSQDLDAERQVILEEINMHEDTPDDLVHDVLNETLWPDHPLGRPILGT
                     VESITSATREQIRRYYRRHYVPGSFVVSAAGDLQHEVLLDLISEHMDTGPVRRDGPND
                     GSSLGGWNMRRAGHAPTPSGQVSVRRRKTEQAHICLGTNGLSRTDPDRFAFGVVNNAI
                     GG"
     gene            complement(843..2105)
                     /locus_tag="VE646_11970"
                     /pseudo
     CDS             complement(843..2105)
                     /locus_tag="VE646_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006236764.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
     gene            2083..>2322
                     /locus_tag="VE646_11975"
     CDS             2083..>2322
                     /locus_tag="VE646_11975"
                     /inference="COORDINATES: protein motif:HMM:NF015671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80740.1"
                     /translation="MYRGRIQEALVPGKQDRNQTLDQVKEDLKTHLLETWGDQLFAER
                     ASQISPAFKELQKKVMRERVLSDGLRLDGRKVDEIR"
BASE COUNT          315 a          812 c          816 g          379 t
ORIGIN      
        1 cccccgatcg cgttgttcac cacgccgaac gcgaaccggt cggggtcggt ccgggacagg
       61 ccgttggtcc ccaggcagat gtgcgcctgc tcggtcttgc gccggcgcac gctgacctgc
      121 cccgagggcg tgggggcgtg cccggcccgg cgcatgttcc acccgcccag cgaggagccg
      181 tcgttggggc cgtcccggcg gaccgggccc gtgtccatgt gctcggagat caggtccagc
      241 aggacctcgt gctgcaggtc cccggcggcg gagaccacga agctccccgg gacgtagtgc
      301 cgccggtagt accgacggat ctgctcgcgg gtggccgagg tgatgctctc cacggtcccc
      361 aggatcggcc ggcccagcgg gtgatcgggc cacagggtct cgttcaggac gtcgtggacc
      421 aggtcgtcgg gggtgtcctc gtgcatgttg atctcctcga ggatcacctg ccgctcggcg
      481 tccaggtcct gcgagcggat caccgagtgc tggagcatgt cgcacaggtg ctccacggcc
      541 atcggcaggt cgcggtcctg cacccgcgcg tagaagcagg tgtactcctt ggcggtgaag
      601 gcgttcacgt ccccgcccac cgcgtcgaag gcctcggcga tgtcccgggc ggtgcggctc
      661 ctggtcccct tgaacagcag gtgctccagg aagtgcgagg acccgctgat ccgggggggc
      721 tcgtcgcgcg agccggccag cacccagaag cccagggcca ccgagcggac gccgggcatc
      781 cgctcggtga cgacccgcag ccccgtcccg aactcggtcc gccggatcgg catgcgtggg
      841 ctctaggagc ggtcccgccg ctctcggtcg cggtcccgcg gtccccggcg gtggtcgcgg
      901 tcgcgccccc ggtggccgtc gcggtcccgg cgctcgcggc ggggctgctc ctcgacctcc
      961 atgccctcgg gcacctccca ctcggggccg atcggcttca ggctgatctt gcccatctgg
     1021 tcgatgtcct ggatctcgac ctcgatcgtg tcgccctcct tgaccgcctc ctccacgctg
     1081 gacaggcgct tgccccggcc gagcttggag atgtggacca ggccgtcgcg tcccggcacc
     1141 aggttcacga acgccccgaa ggtggtggtc ttgaccacct tgccggtgaa ccgctcgccc
     1201 accgagagcg gacgggggtt cgcgatttcg tcgatcatcc gggcggcctc gtcggccccc
     1261 tggccctcct tggagccgat gaacacggtg ccgtcgtcct ggatgtcgat gtcggccccg
     1321 gtcatggcga tgatctcgtt gatccgcttg cccttggggc cgatcacctc gccgatcttg
     1381 tccaccggga tctggatcgt gaggatcctg ggggccttgg ggttcacctc ctcgcggggc
     1441 tcggggatcg tgcgcagcat cgtgtccaga atgaacagcc gggcctcgcg ggcctgcttc
     1501 agcgcctgcg ccagggcctc ggccggcagg ccggtgacct tcatgtcgag ctggatcgcg
     1561 gtgacccagt cccgcgtgcc ggccaccttg aagtccatgt cgcccagcgc gtcctcggcc
     1621 cccaggatgt cggtgagggt cacgtatcgc ccatcctgcg cgatcatgcc catcgcgatc
     1681 ccggccacgg gggacttgat cggcaccccg gcgtccatca gggacagggt cgaggcgcac
     1741 acgctggcca tcgaggtcga cccgttcgag gacagcacgt cggagaccag gcgaagcgcg
     1801 taggggaact cctcctcggt ggggatcacc gggaccaggg cccgctcggc cagggccccg
     1861 tgcccgatct cgcgccggcg gggactgccg atccgaccgg tctcgccggt ggagaagggc
     1921 gggaagttgt agtggtggat gtaccgcttg gagtcctcgg gatccagggt gtcgatcatc
     1981 tgggtcatcc gcagcatccc cagcgtggtc acgttcagga cctgggtctc gccccgctgg
     2041 aagatcgagg agccgtgggc ccggggcacc acggtcacct ccgtgtacag gggccggatc
     2101 caggaggccc tggtccccgg caagcaggac cgcaaccaga ccctggacca ggtcaaggag
     2161 gacctcaaga cccatctgct cgagacctgg ggcgaccagc tcttcgccga gcgcgcctcg
     2221 cagatctcgc cggccttcaa ggagctgcag aagaaggtga tgcgcgagcg ggtgctctcc
     2281 gacgggctgc gcctggacgg gcgcaaggtg gacgagatcc gg
//
LOCUS       DASQQQ010000320        13783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_300406, whole genome shotgun sequence.
ACCESSION   DASQQQ010000320 DASQQQ010000000
VERSION     DASQQQ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13783
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300406"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VE646_11980"
     CDS             <1..223
                     /locus_tag="VE646_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZD80741.1"
                     /translation="YKTEAAIAKLYASEAAVTAAREAVQIHGGYGYIEEFPVARFYRD
                     AKILEIGEGTSEIQRLLIARDLGLPGTEL"
     gene            402..1568
                     /locus_tag="VE646_11985"
     CDS             402..1568
                     /locus_tag="VE646_11985"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZD80742.1"
                     /translation="MAQDRIGKTARRRATVLLAVLMLLSGLAPGASADPKAKLQQARA
                     RLEQIKKEITAQQAVLNQLSAQAAALAQRLDEAQAKYEYITSQLQDTRQKLQEATDRF
                     HAVQTQLNSRTRETYMDGPGSPLEFLLGATSFTDLSDRVEFVDAVTQTDADLANQVQN
                     LRNELAAQQHDQQKLQAKQAEVLRGLQADQAQLDAQFVQQRSVLDDIQAKKAEAEKLV
                     SKATREYKQWLRSLTAPVDIANNPFHVCPVDQPRAVYDGFGAPRYAGGYHPHAGNDIV
                     APPGTPIRAPFDGTARSSYNTLGGNAVYVYGPQGYVYNAHLSQYSSSSDGSVRAGEII
                     GYVGNTGDAQGGVTHDHFEWHPNAMPSDWQKSPYGYSAIGTAVNPYPLLAQVCL"
     gene            1635..3731
                     /locus_tag="VE646_11990"
     CDS             1635..3731
                     /locus_tag="VE646_11990"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HZD80743.1"
                     /translation="MLPARAFPAPTRVHRRLLPLAAAIAIVLLTPQVALAHATPVSSQ
                     PEPGAELGTAPGVVTIRFSEPLNRGLSSITVIDPNGRAFRGTPSDDRTIQAPLSTNAF
                     GTYRVQWTTVSTVDGHTLRGSFTFGVGAAGVGQGETGGGDEPGAADLAIGVARAVEDL
                     ALLFCIGLLMVGALASRRPSYPWAIRDLRWVLGVLLAAGVAVVLGEALLAAQGSIGAV
                     GSFLTTGTPGTARLLRVGLEATAVGLAFGRPRGRRAIGGVVAAAAGALAWSGHAAAAS
                     PQWAGVLVDWAHLLSAGVWAGSIVAIASIRPPEGWRSSEGRQLLDRFSPVGLAAFAVT
                     VGLGAVRGWQELGALSELIHSAYGRVLLIKVAAVAVMIPLSVLMWRRALRSPRVEARL
                     VVVVVLATAVLGAFPFPPARVGEGAAAAASAPSALPGPGDLTLGGQAGQVVVGLTLRP
                     GRPGPQDLLVYLMPLDGEAGAAELRAGLTVDGRPVPLRTCSATCRSARVSLSGGEDVE
                     VKVEGRTGGTARFQIPELPAEDGAPLLDRMMTRMHELRSFRIHERLDSGLTVVDSRYT
                     FVAPYEMTQRSRAPQIYSDIIWIDTTRYVRNGPNEPWKRQVGGRPTKVPAFVWDFFRP
                     FQDAVVLGRQRVQGVPTTRVAFFGESPGVPVWFELWIDRTGLVHEAQMDTRGHFMHHV
                     YSGFDGPIRIAPPSGA"
     gene            complement(3824..5101)
                     /locus_tag="VE646_11995"
     CDS             complement(3824..5101)
                     /locus_tag="VE646_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018804436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZD80744.1"
                     /translation="MLGMGIDWAEEFPVVALGRPGEGVIEVAKVEHTPAAVEGMVGKI
                     AAQKPDPAEVRVVIETSHGMLVERLIHAGYAVVPVNPDLIARRRGPAKKKDDAEDARM
                     ACLIALDRFEVLRPLVPHGEIAGELRAIARHDLRASRDERRLLNRLRADLLVTFPAAM
                     AISGEDLGSPTFLRMLERWPTANALASASREEVEAFARANRHGWPGRFADRVQAALAA
                     DRFVAREYLVRAKRDTIRLGAIQLLGIGAQRRAWEHRMAELLLGAPRWGRAKQPRKEK
                     LGQALPGGEVYLSFPGLGGRLAARVAGEIGDHPEPFATPNSLQCYAGRAPVTRRSGKS
                     ELVVACRLACNRYLADAVQQWAFCSLRRSGWARDFYDAQRAKGKGHHAALRAPGNRWL
                     EILWPCLRRGVPYDEALHVANRNRALGLTPKVA"
     gene            5309..6208
                     /locus_tag="VE646_12000"
     CDS             5309..6208
                     /locus_tag="VE646_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZD80745.1"
                     /translation="MSTGHAAASASFRNRGRLRWVLAITAVFLVAEVVGGLLSDSLAL
                     LADAGHMLTDVSGLGLALFAIWLAHRPSRPERSFGWYRVEILAAMVNGTLLLVVSGTV
                     LFEAWQRWSRPPEIASGLMLGVALAGLVANAVSMALLRRGQRESLNLRAAYLEVLGDL
                     LGSLAVIVAAVTIALTGLRRADAVASAAIGLLILPRTWSLLRDAVDVLLQATPKGVDL
                     SEVRRHIAGTDHVEDVHDLHVWTITSGMNVVSAHVVLSDGADPARVLDDLCACLSDDF
                     DIEHSTFQLETRDRRRVEESSHP"
     gene            6376..7239
                     /locus_tag="VE646_12005"
     CDS             6376..7239
                     /locus_tag="VE646_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018549101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HZD80746.1"
                     /translation="MRAEDVTRVGVVGCGVMGSGIVEVCARAGLDVRFLEADPGLAER
                     GRSRIETSMLRAVERGKLSEDEREAALGRVSATTEVEDLHDRELVIEAATEDYETKRE
                     LFRRLEEVTPPDAVLASNTSSIPIADLGAVTKRPDRVLGLHFFNPVPVMGLIEIVRSI
                     TTSEETVEFGRALGVMLGKTTVLSRDRAGFIVNMLLIPYLNGAVRMLEDGFATREDID
                     TAIHLGLNHPMGPLRLIDLIGVDTTLFVANVLYEEFKEPLYAPPPLLKRMVTAGRLGR
                     KSGHGFYEYEA"
     gene            complement(7236..7736)
                     /locus_tag="VE646_12010"
     CDS             complement(7236..7736)
                     /locus_tag="VE646_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HZD80747.1"
                     /translation="MKTSTRASTQAHAPAARWPVPASLVIDAGGLAVSAYLTIAHFTR
                     PSLLACSTGGTINCEAVTTSAQSTFLGIPVAVLGLAWFAAMTLLCLPAAWRSASRSLH
                     LLRLAGSIVGIGFALWLVYAELVIIRAICLWCSAAHVFAFALFVVVAAASPALLFADD
                     EPLESR"
     gene            complement(7745..8635)
                     /locus_tag="VE646_12015"
     CDS             complement(7745..8635)
                     /locus_tag="VE646_12015"
                     /inference="COORDINATES: protein motif:HMM:NF017831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF929 family protein"
                     /protein_id="HZD80748.1"
                     /translation="MARSTSNRSRSKPTARRAPARRAPGRRARTSWWIAIAGVVAVVS
                     ALVAIVALGRSPAPATSASTRAIREVTHVPARALASAVPGSLPQVHALPPGTPPVETG
                     GKPVLTYVGAEYCPYCAAERWPLVVALSRFGRFSHLEASASASNDVFPSTPTLSFHGA
                     AYTSPYLSLSAVETSTNRPAALGGYTSLDAPTAQQQELLNRYDTTRYTGTQGAIPFVM
                     VGSRYVWAGATYSPAVLGGLSFDQIAARLADPTTTVGRTIDAQANAITAMICQLTGDQ
                     PSPVCSTPSVARAVSSLPGS"
     gene            complement(8646..9602)
                     /locus_tag="VE646_12020"
     CDS             complement(8646..9602)
                     /locus_tag="VE646_12020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZD80749.1"
                     /translation="MKVSLITTVRNAGGRVEPFLRAISAQTRPPDEMIVVDGGSTDGT
                     LETLRRAEAITLIEEPGANIARGRNVAVAASAHEVIAVSDADCEPEPEWLERLVATLE
                     EGADVAMGFYRPIARRFWQQVLACVNLPDAERIDPARFMPSSRSVAFRRGALEAAGGY
                     PEWLDIGEDMYVDHRWRELGLDLRFVPQAVVRWPMRANLSETWTQYLRYARGDAIAGM
                     HADRHALRFAVYGGTLLAWSSRSRLPKAVTLVGATAYAAAPARRVLRRFEGSAERARA
                     LAVLPAVMAFLDVAKIAGYVAGLIAGRRRSAPGDTAATVGTR"
     gene            complement(9599..10735)
                     /locus_tag="VE646_12025"
     CDS             complement(9599..10735)
                     /locus_tag="VE646_12025"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZD80750.1"
                     /translation="MVAVTRVAVHVDQVWFPAPGGIGTYVRNLVPALSRADPGLDLTL
                     FHARFGTTEVPERWIREFWVEELPGSIRSLYPRWALLGRPALPSSLAGADLVHATNPA
                     GVPPPGPGRRLVVTVHDLAFLRFPSLFARNWRWQYRLGLRAAVRRADAIITPSRATAE
                     DLLSRSSVEPDRLHVVPLASSLDAAPADPEPVLLRLKIRRPYLLFVGTLEPRKNLVRL
                     VRAYRRVAGGGIPHGLVLAGPLGWHHESLMREIALRGPGEIVMTGPLPPGDLDALYRA
                     ASAFVYPSLYEGFGLPVVEAMARGVPTVTSDTSSLPEVAGEAALTVGPTSVSELAVAL
                     ERVLGDRELSRRLGAAGRERAERFSWAETARLTLQVYEHVLGAK"
     gene            complement(10752..11048)
                     /locus_tag="VE646_12030"
     CDS             complement(10752..11048)
                     /locus_tag="VE646_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZD80751.1"
                     /translation="MGATRTPAGVRSVGRAEAAFRDEGCSPPRPWRNAPGDTYGRHSH
                     PYHKVLFCLEGSIVFHCPEGDVALGAGDRLDLDPGTEHAATVGPHGVSCVEASR"
     gene            complement(11119..11379)
                     /locus_tag="VE646_12035"
     CDS             complement(11119..11379)
                     /locus_tag="VE646_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80752.1"
                     /translation="MTHDRQLQLPPGLSPEEERSTLRALERYFGQESPHPHPWVLAGR
                     MEATGWGALQARRHADSGWPMMRHARFVRPGVPTMTGRGDAR"
     gene            complement(11387..12970)
                     /locus_tag="VE646_12040"
     CDS             complement(11387..12970)
                     /locus_tag="VE646_12040"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HZD80753.1"
                     /translation="MAKIEEQPISLVTVEDRIEELRRRKAAMLDERRKEAVRKQHERG
                     KLTARERIDLLFDAGSFQETDPLVTHRAHDFGLERNRPAGDGVVTGYGTVDGRRVFAA
                     SQDFTVFGGSLGEVHAQKICKVLDLAASTGSPFIQVNDSGGARIQEGAASLAGYGHIF
                     ERNVRASGVIPQISVITGPCAGGAVYSPALTDFTFMVRETSHMFITGPDVIKTVTGED
                     VTFEELGGAMTHASRSGVASFVATDEEECLAMVRHLLSYLPSNNLEDPPSYAPVDDPQ
                     RHDEGLTHLIPDSAREPYDMHEVIRRVVDDGDFFEVFPYWAMNIVVGFARLDGRTVGV
                     VANQPKVLAGTLDIDSSEKASRFVRVCDAFNVPLVTFVDVPGFLPGAAQEYGGIIRRG
                     AKLLYAFAEATVPRLTVITRKAYGGAYLVMNSKHLRADVAFAWPTAEIAVMGPDAAVK
                     VVFKKEIEKADDPMARRGELIDEYERKFANPYVAAERGFVDDVIEPAETRPRLIRALN
                     MLATKREAVPARKHGNIPL"
     gene            complement(13010..>13783)
                     /locus_tag="VE646_12045"
     CDS             complement(13010..>13783)
                     /locus_tag="VE646_12045"
                     /inference="COORDINATES: protein motif:HMM:NF021386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HZD80754.1"
                     /translation="RTSTTSTSGPKNQREARTNSAPSMPPLRATQPRHQLGREGGPGT
                     GPDQALERPTLEGLSAVVASTERMVGVATDMAETPEQILRALADPERLAMAGTLAQTP
                     RTATELASDLHLSVARVHKHLNRLTTTGVVRLQPDRRTYRLDPETLRWAAEEVGPPRD
                     TGIALGAADESEESVLRAFFRKGRLTEIPAKASKRRIVLERIALEFDPGVRYDEREVN
                     VIVGRFFTDHAALRRYLVEEGLLSRDHGVYWRTGGRVEL"
BASE COUNT         1940 a         4898 c         5020 g         1925 t
ORIGIN      
        1 ctacaagacc gaggccgcca tcgccaagct ctacgccagc gaggccgcgg tgaccgccgc
       61 ccgggaggcg gtgcagatcc acggcggcta cggctacatc gaggagttcc ccgtggcccg
      121 cttctaccgg gacgccaaga tcctggagat cggtgagggc accagcgaga tccaacggct
      181 gctgatcgcc cgcgaccttg ggctgcccgg caccgagctc tgaggtgcga ggccgatccg
      241 gccggtcccc gagcggaggg gcccgatcgg aggggcccga tcggcagagg gggggtcctg
      301 ggtcgtcgac caagatggtt gaacccccgg atgaaccggg tcaccctgca tggcgggatg
      361 attgacatct ccgccgatgg tcgagtagga aagggctccc catggcccag gatcggatcg
      421 ggaagacggc ccggcgccgc gctacggtgc tgctcgccgt cctgatgctt ttgtccgggc
      481 tcgcgcccgg ggcgagcgcc gaccccaagg ccaagctcca gcaggcgagg gcccggctcg
      541 agcagatcaa gaaggagatc acggcccagc aggcggtgct gaaccagctc tcggcgcagg
      601 ccgcggccct ggcacaacgg ctcgacgagg ctcaggccaa gtacgagtac atcacgtccc
      661 agctgcagga cacgcggcag aagctgcagg aggccaccga tcggttccac gccgtgcaga
      721 cgcagctgaa ctcccggacc cgagagacgt acatggacgg cccggggagc ccgctggagt
      781 tcctgctggg cgcgacctcg ttcaccgatc tgtccgaccg ggtggagttc gtggacgcgg
      841 tcacccagac cgacgcggac ctcgcgaacc aggtccagaa cctgcggaac gagctcgcgg
      901 cgcaacagca cgatcagcag aagctccagg ccaagcaggc cgaggtcctg cggggcctgc
      961 aggccgatca ggcccagctg gacgcgcagt tcgtccagca gaggtcggtg ctggatgaca
     1021 tccaggccaa gaaggccgag gcggagaagc tcgtgagcaa ggccacccgc gagtacaagc
     1081 agtggctgcg ctccctcacc gctcccgtgg atatcgcgaa caacccgttc cacgtctgcc
     1141 cggtggacca gcccagggcc gtctacgatg ggttcggcgc cccgcggtac gcgggtgggt
     1201 accacccaca cgccggcaac gacatcgtgg caccaccggg gacccccatc cgcgccccct
     1261 tcgacgggac ggcgcgctcc tcgtacaaca cgctgggcgg caacgccgtc tacgtctacg
     1321 gtccgcaggg ctacgtgtac aacgcgcacc tgtcccagta ctcgtccagc tccgacggct
     1381 cggtgcgggc cggcgagatc atcggctacg tgggcaacac gggcgacgcc cagggcgggg
     1441 tgacccacga ccacttcgag tggcacccga acgccatgcc ctcggactgg cagaagagcc
     1501 cctacggcta ctcggcgatc ggcaccgccg tgaaccccta ccccctcctc gcgcaggtct
     1561 gcctctagcg gatcgggacg gcaccgcccg gcagaccgag gggagggaag cgctgctcgt
     1621 agactccgtt caccgtgctc cccgctcgag ccttccctgc acccacccgc gtccaccgcc
     1681 gcctcctgcc gctcgccgca gccatcgcca tcgtcctgct gacgccgcag gtggctctgg
     1741 cccacgccac cccggtctcc tcacagcccg agccgggcgc cgagctgggg acggcgccgg
     1801 gggtggtcac gatccggttc tccgagccgc tgaaccgggg gctgtcgtcg atcacggtga
     1861 tcgatcccaa cgggagggcc ttccgcggga ccccctcgga cgaccggacg atccaggcgc
     1921 cgctgtcgac gaacgcgttc gggacctacc gggtccagtg gaccaccgtc agcacggtgg
     1981 acggccacac cctgcgcggt tccttcacct tcggggtggg ggcggccggc gtggggcagg
     2041 gggagacggg tggcggcgac gagccgggcg ccgccgacct ggcgatcggg gtggcccgcg
     2101 cggtggagga cctggccctc ctgttctgca tcgggctgct gatggtgggg gcgctggcgt
     2161 cgcggcgtcc ctcctacccc tgggcgatcc gggatctccg atgggtcctg ggggtcctct
     2221 tggcggccgg ggtagcggtg gtcctgggcg aggccctgct cgcggcgcag ggctcgatcg
     2281 gggcggtggg gtccttcctc accaccggga cgccgggcac cgcccggctg ctccgcgtgg
     2341 ggctcgaggc gacggcggtg ggtctggcct tcggccggcc acggggccga agggcgatcg
     2401 ggggcgtggt ggcggccgcg gccggtgcgc tcgcgtggtc gggccacgcc gcggcggcga
     2461 gcccgcagtg ggcgggcgtc ctggtggact gggcgcacct gctctcggcc ggcgtgtggg
     2521 ccggaagcat cgtggccatc gcgtcgatcc ggccacccga gggctggcgg agctcggagg
     2581 ggcggcagct gctggaccgg ttcagcccgg tggggctggc cgccttcgcg gtgaccgtgg
     2641 gcctgggggc cgtgcgcggc tggcaggagc tcggggccct gtccgagctg atccactcgg
     2701 cgtacgggcg tgtgctgctg atcaaggtcg ctgccgtggc cgtcatgatc cccctgtcgg
     2761 tgctgatgtg gcggcgtgcg ctccgctctc cgagggtcga ggcccggctg gtggtggtgg
     2821 tcgtgctcgc cacggcggtg ctgggcgcct tccccttccc gcccgcccgc gtgggcgagg
     2881 gagcagcagc ggcggcgtcc gccccctcgg cgctccccgg ccccggtgat ctcaccctgg
     2941 gcgggcaggc gggtcaggtg gtggtgggcc tcaccctgcg gcccggccgc cccggtcctc
     3001 aggacctgct cgtgtacctg atgccgctgg acggcgaggc aggagccgcc gagctgcggg
     3061 cggggctcac cgtggacggc cggcccgtcc cgctgcggac ctgctcggcg acctgccgga
     3121 gcgctcgcgt gtccctgagc ggtggtgagg acgtcgaggt caaggtggag ggcaggaccg
     3181 gcgggaccgc ccggttccag atcccggagc tgccggccga agatggggcc ccgctcctgg
     3241 atcggatgat gacccggatg cacgagctgc ggtccttccg gatccacgag cgcctggact
     3301 caggcctcac ggtggtggac tcgcgctaca cgttcgtggc gccgtacgag atgacccagc
     3361 gctcgcgagc gccgcagatc tactcggaca tcatctggat cgataccacg aggtacgtcc
     3421 gcaacggacc caacgagccc tggaagcggc aggtcggcgg caggcccacg aaggtgcccg
     3481 cgttcgtgtg ggacttcttc cggcccttcc aggatgcggt cgtgctcggc aggcagcggg
     3541 tccaaggggt gcccaccacg cgggtggcct tcttcgggga gagcccgggc gtgccggtct
     3601 ggttcgagct gtggatcgac cggaccggtc tggtccacga ggcccagatg gacacgcgag
     3661 gccacttcat gcaccacgtc tactcgggct tcgacgggcc gatccgcatc gcgccgccga
     3721 gcggggcctg aggtgaacga ggtcgcctcc ccaggcgtgg acggacccgg cgtgcggatg
     3781 gaaccatggt cgtatgagac accctttgtc aaccacctcg gagtcaggcc actttagggg
     3841 tgaggccgag agcgcggttc cggttggcga cgtggagggc ctcgtcatac gggactcccc
     3901 ttcgcaggca gggccacagg atctcgagcc accggttccc cggcgctctg agcgcggcgt
     3961 gatgtccctt gcccttggct cgctgggcgt catagaagtc ccgggcccac cccgatctgc
     4021 gcaggctgca gaacgcccac tgctgcaccg catcggccag gtaccggttg caggccaggc
     4081 ggcacgccac caccagctcg ctcttgccgg agcgccgggt gaccggggcc cggcccgcgt
     4141 agcattgcag cgagttcggc gtggcgaacg gttcgggatg atcgccgatc tcaccggcca
     4201 ccctggcggc gaggcggcca ccgaggcccg gaaagctcag gtagacctcg ccgccaggga
     4261 gcgcctgccc cagcttctcc ttccggggct gcttggctcg cccccacctc ggggccccca
     4321 gcaggagctc ggccatccgg tgctcccagg cccggcgctg ggccccgatg cccagcagtt
     4381 ggatggcccc caggcggatg gtgtctcgct tggcccggac cagatactcc cgggccacga
     4441 accgatcggc cgccagggca gcttgaaccc ggtcggcaaa gcggcccggc cacccgtgcc
     4501 ggttggcccg ggcgaaggct tccacctcct cccgggacgc actggccagc gcgtttgccg
     4561 tgggccagcg ttccagcatc cgcaggaacg tgggcgagcc caggtcctcc ccggagatgg
     4621 ccatggctgc ggggaatgtg accaagaggt cggcccgcag gcggttcagc agccggcgct
     4681 cgtcccggga cgcccgcagg tcgtggcggg cgatggcccg cagctcgccc gcgatctccc
     4741 cgtggggaac gaggggacgc agcacctcga accggtccag cgcgatcagg caggccatcc
     4801 gggcgtcctc ggcgtcgtcc ttcttcttgg ccgggccgcg ccggcgggcg atgagatcgg
     4861 ggttgaccgg caccaccgcg taccccgcgt ggatcaagcg ctccaccagc atgccgtggc
     4921 tggtctcgat gaccacccgg acctcggctg ggtcgggctt ctgggcggcg atcttgccga
     4981 ccatgccctc gacggccgcc ggcgtgtgct ccaccttggc gacctcgatc acgccctcgc
     5041 cggggcggcc cagggccacc acggggaact cctcggccca atcgatcccc attccgagca
     5101 tgggtctccc ttcttcgttc gggggaaggc cagagggaag gaggtggagg cacggcgccg
     5161 tgctgatgga ccagtcctcg tgggacgaca cccttgtggg cgtcgggcct ccgagaccgg
     5221 ccccgaggga agccgtctca tctcagtcct cgtgggacaa gcacaggaag ctttccctcg
     5281 aggcgatctc gtgggaggag gttaagccat cagcacgggc catgccgccg catcagcatc
     5341 gttccggaac cggggccggc tccggtgggt gctggcgatc accgcggtct tcctggtggc
     5401 cgaggtggtg ggcggcctgc tctcggacag cctggcgctc ctcgccgacg ccgggcacat
     5461 gctcaccgac gtgtccggcc tggggctggc cctgttcgcg atctggctcg cccaccgacc
     5521 gtccaggccc gagcgctcct tcggctggta ccgggtggag atcctggccg ccatggtcaa
     5581 cgggacgctc ctgctcgtgg tctccgggac ggtcctgttc gaggcctggc aacgctggtc
     5641 ccggcctccg gagatcgcgt cggggttgat gctcggcgtg gccctggccg gcctggtcgc
     5701 gaacgccgtc tccatggccc tgctgcggcg agggcagcgc gagagcctga acctgcgtgc
     5761 cgcctacctc gaggtgctgg gggacctgct gggctccctg gccgtcatcg tggccgccgt
     5821 gaccatcgcc ctcaccgggc tgcgtcgtgc ggacgccgtc gcctccgccg cgatcgggct
     5881 cctgatcctg ccccggacct ggagcctgct ccgggacgcc gtcgacgtgc tcctgcaggc
     5941 cacccccaag ggggtggacc tctccgaggt ccgtcgccac atcgcgggga ccgatcacgt
     6001 ggaggacgtg cacgacctgc acgtatggac catcaccagc ggcatgaacg tcgtctcggc
     6061 gcacgtggtc ctgtccgacg gggccgatcc ggcgcgggtc ctggacgacc tgtgcgcgtg
     6121 cctctcggac gacttcgaca tcgaacacag cacgttccag ctggagaccc gggatcggcg
     6181 ccgggtggag gagtcgagcc acccctgagg ggccgcatcg ggccgctcgg cgccccttgc
     6241 ggtgtcccct ggctcaccct gtggcccacc acttctcgtt cctgctgatc gagggacagg
     6301 gcggtagcct gtgccgggga gggccaccga cctctccggc catcccggac cggccgacga
     6361 tcggaggagc gcgtgatgcg agcggaggac gtgacgcggg tcggcgtcgt gggctgcggc
     6421 gtgatgggct cgggcatcgt ggaggtctgc gcccgggccg ggctggacgt ccgtttcctg
     6481 gaggccgacc cggggctggc ggagcggggc cgctcccgga tcgagacctc gatgctccgt
     6541 gccgtggagc ggggcaagct ctccgaggac gagcgcgagg ccgcgctcgg acgggtgagc
     6601 gccaccaccg aggtggagga cctccacgac cgcgagctcg tgatcgaggc cgccaccgag
     6661 gactacgaga cgaagcgcga gctgttccgg cggctggagg aggtcacccc tcccgacgcc
     6721 gtgctggcca gcaacacgtc gtcgatcccc atcgccgacc tgggagccgt gacgaagcgg
     6781 ccggaccggg tcctcggtct ccacttcttc aacccggtgc ccgtgatggg cctgatcgag
     6841 atcgtccggt ccatcaccac ctcggaggag acggtcgagt tcggacgggc gctcggggtg
     6901 atgttgggca agaccacggt gctgtcccgg gaccgggccg ggttcatcgt gaacatgctg
     6961 ctgatcccgt acctgaacgg cgccgtgcgg atgctcgaag acggcttcgc cacgcgcgag
     7021 gacatcgaca ccgcgatcca cctcggcctg aaccacccga tgggcccgct gcgcctgatc
     7081 gatctgatcg gggtggacac cacgctgttc gttgccaacg tgctgtacga ggagttcaag
     7141 gagccgctgt acgcccctcc gccgttgctg aagcgcatgg tgacggccgg gcgcctgggc
     7201 cgcaagagcg gccacggctt ctacgagtac gaggcctagc ggctctcgag cggctcgtcg
     7261 tcggcgaaca gcagcgccgg gctggccgcg gcgaccacca cgaacagggc aaaggcgaag
     7321 acgtgcgcgg cgctgcacca caggcagatc gcgcggatga tcaccagctc cgcgtagacc
     7381 agccacaggg cgaacccgat cccgacgatc gacccggcca ggcggagcag gtgcaggctc
     7441 cggctcgcgg agcgccaggc cgccggcagg cacaggaggg tcatggcggc gaaccaggcc
     7501 agcccgagca ccgccacggg gatccccagg aaggtggact gggcgctggt ggtcaccgcc
     7561 tcgcagttga tcgtgccgcc ggtgctgcag gccaggaggc tggggcgggt gaagtgggcg
     7621 atcgtgaggt acgcggacac cgcgagaccg cccgcatcga tcaccaggct cgccggcacc
     7681 ggccatcggg ccgccggggc gtgggcctgc gtcgaggccc gggtcgaggt cttcaccggc
     7741 ccgctcatga ccccggcaac gaggagaccg cccgtgccac ggagggcgtg gagcagaccg
     7801 gcgagggttg gtccccggtc agctggcaga tcatcgccgt gatcgcgttg gcctgcgcat
     7861 cgatcgtccg gcccaccgtc gtggtggggt cggcgagccg ggcggcgatc tggtcgaagg
     7921 acaggccgcc caagacggcc ggcgaatagg tcgcgccggc ccacacgtag cggctgccca
     7981 ccatcacgaa ggggatcgct ccctgggtgc cggtgtagcg cgtggtgtcg tagcggttga
     8041 gcagctcctg ctgctgggcg gtcggcgcgt ccagggaggt gtacccgccc agggccgcgg
     8101 gtcggttggt cgacgtctcg acggccgaga gggagaggta cgggctggtg taggcggcgc
     8161 cgtggaagct cagggtgggc gtgctgggga agacgtcgtt ggaggccgag gcgctggcct
     8221 cgaggtgaga gaacctcccg aagcggctca aggccaccac cagcggccag cgttcggcgg
     8281 cgcagtacgg gcagtactcg gcgcccacgt acgtgagcac cggcttcccg ccggtctcca
     8341 ccggcggcgt ccccggtggc agggcatgga cctggggaag cgatcccggc accgccgagg
     8401 ccagggctcg ggccgggacg tgggtgacct cacggatcgc cctggtcgag gcggaggtgg
     8461 ccggggccgg ggaacggccg agggcgacga tcgcgacgag cgccgagacc accgccacca
     8521 cgccggcgat cgcgatccac cacgaggtcc gggcacgccg ccccggggca cgccgggccg
     8581 gggcacggcg tgcggtcggc ttggagcgac tccggttgga agtggaacga gccatgggac
     8641 gatccctacc tggtaccgac tgtagcagcg gtgtcgccgg gcgccgagcg ccgccggccg
     8701 gcgatcagcc cggccacgta gccggcgatc ttcgccacgt ccaggaaggc catgaccgcc
     8761 gggaggacgg ccagggcgcg ggcccgctct gcggacccct cgaaacgacg caggacccgt
     8821 cgggcggggg cggccgcgta cgcggtggcc cccaccagcg tgacagcctt cggcaggcgg
     8881 ctccgcgaag accaggccag cagggtccct ccgtacacgg cgaaccggag cgcgtgccgg
     8941 tcggcgtgca tcccggcgat cgcgtccccg cgcgcgtagc gcaggtactg ggtccaggtc
     9001 tcggagaggt tggctcgcat gggccatcga acgaccgcct gaggcacgaa ccgcaggtcc
     9061 aggccgagct cgcgccaccg gtggtccacg tacatgtcct cgccgatgtc gagccactcc
     9121 gggtatccgc cggccgcctc cagggctccg cgacggaacg ccaccgagcg tgacgaaggc
     9181 atgaaccggg ccggatcgat ccgctcggcg tcgggcaggt tcacgcaggc cagcacctgc
     9241 tgccagaacc gccgtgcgat cgggcggtag aagcccatgg ccacgtcggc gccctcctcg
     9301 agggtcgcca ccagccgctc cagccactcc ggctcgggct cgcagtccgc gtcgctcacg
     9361 gcgatcacct cgtgggccga ggccgccacg gccacgttgc ggccccgggc gatgttcgcc
     9421 cctggctcct cgatcagggt gatggcctcg gcccgccgga gggtctccag cgtgccgtcc
     9481 gtggaccccc cgtccaccac gatcatctcg tccggcggcc gggtctgggc cgagatcgcc
     9541 cgcaggaacg gctccacccg tccccccgcg ttccgcaccg tggtgatcag cgagaccttc
     9601 acttcgcccc cagcacgtgc tcgtacacct ggagggtgag ccgggcggtc tctgcccacg
     9661 agaagcgttc ggcgcgttcc cgccctgcgg cccccagacg ccgactcagc tcccgatcgc
     9721 ccagcacccg ctccagggcc accgccagct cgctcaccga cgtggggccc accgtcagcg
     9781 ccgcctcccc ggccacctcg ggcagggagg aggtgtcgga ggtcaccgtg ggcacgcctc
     9841 gggccatcgc ctccaccacc gggagaccga agccctcgta caacgagggg tacacgaagg
     9901 ccgaggcggc acgatagagg gcgtcgaggt ccccgggagg cagcgggccg gtcatcacga
     9961 tctcgcccgg gcctcgcagg gcgatctcgc gcatcaggga ctcatggtgc cagccgagcg
    10021 gtccggccag gaccaggccg tgcgggatcc caccccctgc cacccggcgg tacgcgcgga
    10081 ccagccggac caggttcttg cggggctcga gcgtgcccac gaacaggagg tagggacgcc
    10141 ggatcttcag ccggagcagg acgggctcgg ggtcggccgg ggcggcatcg agggaggacg
    10201 cgagcggcac cacgtggagc cggtcgggct cgaccgagct cctcgacagg aggtcctcgg
    10261 cggtggcccg tgacggcgtg atgatcgcgt cggccctccg caccgcggcc cgcagcccca
    10321 gccggtactg ccatcgccag ttccgcgcga acaggctcgg gaaccgcagg aaggccaggt
    10381 cgtgcacggt caccaccagc cgccgaccgg gcccgggcgg cgggaccccc gccgggttgg
    10441 tcgcgtggac caggtcggct ccggccagcg aggacggtag cgccggccgg ccgagcagcg
    10501 cccatcgggg gtagagcgag cggatcgacc ccggcagctc ctccacccag aactcgcgga
    10561 tccaccgctc cgggacctcc gtggtgccga aacgcgcgtg gaacaaggtg aggtcgagcc
    10621 ctggatcggc ccgggacagg gcggggacca ggttccgcac gtaggtgccg atgccgccgg
    10681 gagcggggaa ccacacctgg tccacgtgca ccgccacccg cgtcaccgcg accaccccgt
    10741 gcgcccggtc gtcatcggga ggcctccacg caggacaccc cgtgggggcc gacggtggcg
    10801 gcgtgctccg tgccgggatc cagatcgagc cggtcccccg cgccgagcgc gacgtcgccc
    10861 tcggggcaat ggaagacgat cgacccctcc aggcagaaca ggaccttgtg gtaggggtgc
    10921 gagtgccgcc cgtaggtgtc gcccggagcg ttgcgccagg gacggggcgg cgagcacccc
    10981 tcgtcacgga aggccgcctc ggcccgtccg acgctccgca cgccggcggg ggtgcgcgtg
    11041 gcacccatca ccggtgccgg agggactccg cccgggccgg ccaccggagg cggacgtacc
    11101 cgggtcggac cctcgccctc accgcgcgtc gccccggccg gtcatcgtcg gcaccccggg
    11161 ccggacgaac cgcgcgtggc gcatcatcgg ccagcccgaa tcggcgtggc gacgggcctg
    11221 gagcgctccc cagccggtcg cctccatccg gccggccagc acccaggggt gtgggtgggg
    11281 gctctcctga ccgaagtacc gctccagggc ccgcagggtc gagcgctcct cctccgggga
    11341 gagccccggc ggcagctgca gctggcggtc gtgggtcatc ggcgcctcac agggggatgt
    11401 tgccgtgctt gcgggcgggc acggcctcgc gcttggtggc gagcatgttc agggcccgga
    11461 tcagccgcgg ccgggtctcg gccggctcga tcacgtcgtc cacgaacccg cgctcggccg
    11521 ccacgtaggg gttggcgaac ttgcgctcgt actcgtcgat cagctcgccc cgccgggcca
    11581 tcgggtcgtc ggccttctcg atctccttct tgaagaccac cttgacggcg gcgtccggcc
    11641 ccatgaccgc gatctccgcg gtgggccagg cgaacgcgac gtcggcccgg aggtgcttgg
    11701 agttcatcac caggtaggcc ccgccgtagg ccttgcgcgt gatgaccgtg agccggggca
    11761 ccgtggcctc ggcgaacgcg tataggagct tcgcccctcg gcggatgatc ccgccgtact
    11821 cctgggccgc gccgggcagg aaccccggca cgtccacgaa ggtcacgagc ggcacgttga
    11881 aggcgtcgca cacccggacg aaccgcgagg ccttctcgct ggagtcgatg tcgagggtcc
    11941 cggccaggac cttcggctgg ttcgccacca cccccaccgt tcgcccgtcc agccgggcga
    12001 agcccaccac gatgttcatg gcccagtacg ggaagacctc gaagaagtcg ccgtcgtcca
    12061 ccacccgccg gatcacctcg tgcatgtcgt agggctcccg ggcggagtcc gggatgaggt
    12121 gggtcaggcc ctcgtcgtgc cgctgtgggt cgtcgaccgg cgcataggag ggcgggtcct
    12181 ccaggttgtt cgagggcagg tacgagagga ggtggcgcac catggccagg cactcctcct
    12241 cgtccgtggc cacgaacgag gccacgccgg agcgggacgc gtgggtcatg gcgccgccca
    12301 gctcctcgaa cgtcacgtcc tcgcccgtca cggtcttgat cacgtcgggc ccggtgatga
    12361 acatgtgcga cgtctcgcgc accatgaagg tgaagtccgt gagcgcgggg gagtagaccg
    12421 cgcctcccgc gcacgggccg gtgatcacgc tgatctgcgg gatcacgccg gaggcccgca
    12481 cgttccgctc gaagatgtgc ccgtacccgg ccagggaggc cgccccctcc tggatccgcg
    12541 ccccgcccga gtcgttgacc tggatgaagg gagagccggt ggaggccgcg aggtcgagca
    12601 ccttgcagat cttctgggcg tggacctcgc cgagggagcc gccgaagacc gtgaagtcct
    12661 gggaggcggc gaagacccgc cggccgtcga cggtcccgta gccggtgacc acgccgtccc
    12721 cggcggggcg gttgcgctcc agcccgaagt cgtgcgcgcg atgcgtcacc agagggtcgg
    12781 tctcctggaa cgagcccgcg tcgaacagca ggtcgatccg ctcccgggcg gtgagcttgc
    12841 cccgctcgtg ctgcttgcgg accgcctcct tgcgccgctc gtccagcatc gccgccttgc
    12901 gtcgacggag ctcctcgatg cggtcctcga ccgtcaccag ggagatcggt tgctcctcga
    12961 tcttcgccat ggtcccggag tctaccgccc ctcctgcggc ggaccagggt cagagctcga
    13021 cgcggccgcc ggtccgccag tacacgccgt ggtcccggga caggaggccc tcctcgacga
    13081 ggtaccggcg gagggccgcg tgatcggtga agaagcggcc cacgatgacg ttgacctccc
    13141 tctcgtcgta gcggacgccg gggtcgaact cgagcgcgat ccgttccagt acgatgcgcc
    13201 gcttggacgc cttggccggg atctcggtca gccgcccctt gcggaagaag gctcggagca
    13261 cgctctcctc gctctcgtcg gccgcgccga gggcgatccc ggtgtcccga ggcggcccga
    13321 cctcctcggc cgcccagcgg agggtctccg gatccaggcg gtaggtgcgg cggtccggct
    13381 gcagtcggac caccccggtg gtggtgagcc ggttcaggtg cttgtggacc cgagcgacgg
    13441 acaggtgcag atcggaggcc agctccgtgg ccgtccgggg cgtctgggcc agggtccccg
    13501 ccatggccag gcgctcggga tcggccagcg cgcggaggat ctgctcgggg gtctcggcca
    13561 tgtcggtggc tactcctacc atgcgctccg tggaggcaac caccgcgctc agcccctcca
    13621 gcgtcggacg ctccagggcc tgatcgggac cggtgcccgg cccaccttcc cgcccgagct
    13681 ggtgccgcgg ctgcgtggcg cggagcggcg gcatcgacgg cgccgagttg gtcctcgcct
    13741 ctcgctggtt cttcgggccc gacgtcgaag tcgtcgacgt gcg
//
LOCUS       DASQQQ010000321         5137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_323373, whole genome shotgun sequence.
ACCESSION   DASQQQ010000321 DASQQQ010000000
VERSION     DASQQQ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323373"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VE646_12050"
     CDS             <1..337
                     /locus_tag="VE646_12050"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZD80755.1"
                     /translation="GAISNPSAPVAYLGVVTQDVGPSIAFQLNLPVQSGALVVSTTPD
                     GPASKAGVDAGDVIVSFDGKDVKSSQQLGSLIHQRQPGDQVPVVVVKPDGSRQTVTAT
                     LGTSPNPLA"
     gene            404..601
                     /locus_tag="VE646_12055"
     CDS             404..601
                     /locus_tag="VE646_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018111835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZD80756.1"
                     /translation="MRVRLRNPDRELEVAGDRTVREVLAELGIDPDTVLVIRDRDLLM
                     RQDRVAGDDLLEVRPVISGGS"
     gene            601..1599
                     /locus_tag="VE646_12060"
     CDS             601..1599
                     /locus_tag="VE646_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZD80757.1"
                     /translation="MRCRRCRGPAVVEIRRHNAAFCRDCFLRHVREQVKRAIASYHML
                     EADDRVLVAVSGGKDSLGLWDVLLDLGYRASGLYLGLGIGTYSDRSGDLAHRFSAARE
                     AELLTVDLEADYGFDVPTAGKRGGRSTCAVCGLSKRYVFNRVALEHGFDVVATGHNLD
                     DEAATLLGNTLRWQTEYIARQFPVLPAEQGLVKKVKPLFRLSELETAAYAFLRGIDYV
                     VEECPLVGGNTQLRYKDAMNRLEAGSPGTKAQFLFGYLDRAAPLFARQDDATLGPCQR
                     CGQPTTGRFCAFCRAQAQILGHRLGARQAAGDEHRQVVRESSDEVLPAEIYGGAGT"
     gene            1632..2426
                     /locus_tag="VE646_12065"
     CDS             1632..2426
                     /locus_tag="VE646_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020497789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HZD80758.1"
                     /translation="MFQPGERVLLVDARGRRYMVNRLRPGESWHSHAGAVPHDLILGS
                     QEGVRVHSTGGMGFDCFRPRLVDFVLKMPRGAQVVYPKDLGAILIEGDVFPGTRVLEA
                     GTGSGSLTIALARAVGDGGAVVSYELREEFHLKAARNVEAFFGSQPPWLDLRLGDLRD
                     ISSSERFDRVVLDLPEPWGVLEAVLAVLDRGGVLCAYVPTTIQAQTTVLAMGERGFGQ
                     VQTFEVLHRPWHVTDRSVRPDHRMVAHTGFITVGRLGERVPGGQGS"
     gene            2545..4293
                     /gene="arc"
                     /locus_tag="VE646_12070"
     CDS             2545..4293
                     /gene="arc"
                     /locus_tag="VE646_12070"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018723034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HZD80759.1"
                     /translation="MPESDPREQQERYEKQIHELQTQVRFLEDEVALLRRRLTNAPRQ
                     VKILEEKLLETKGDLSRAVTQNEKLAGALAAERDRIESLREEVEKLSQPPATFGVYLS
                     RNDDGSVDVFTAGRKMRVNVAPELDLVQLHRGAEVILNEALNVVDILRPDVSGEVMRV
                     KDRLGTERVIVVGHGDEELVATLGHGLHSEPIRAGDPLLVDRRSNVALERLPKEEVEE
                     LVLEEVPDVSYEDIGGLEGQIEAIRDAVELPFLYAELFHEHQLEPPKGVLLYGPPGCG
                     KTLIAKAVAKSLADKVRERTGRPDAHSYFLNIKGPELLNKYVGETERQIRQIFQRAKE
                     RSEEGLPVIVFFDEMDSIFRTRGSGISSDVENTIVPQLLSELDGVETLKNVIVIGASN
                     REDLIDPAILRPGRLDVKIKIERPSAAQAADIMGKYLTATVPIHPDEVRRAGDVTQAV
                     PRMIQGTVDRMYAQTDDNRFLEVTYASGDKEVLYFKDFNSGAMIENIVRRAKKEAIKR
                     FLSTGEKGIKTEDLLLAIRDEFKENEDLPNTTNPDDWARISGKKGERIVYVRTLLGDG
                     GDEGRQVERVSAGQYL"
     gene            4303..>5137
                     /locus_tag="VE646_12075"
     CDS             4303..>5137
                     /locus_tag="VE646_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HZD80760.1"
                     /translation="MAIPKVIGTETEYGISAVGAPDFNPVLSSSLLISTYAGTLRRIR
                     WDYEQESPLRDARGFEPVQMREPVEEDLGLANVILPNGARYYVDHAHPEYSTPECADP
                     RELVIHDKAGERILERSLQELHRALPPGQRIAIYKNNTDGKGNSYGTHENYLVDRATP
                     FANIVRDFTPYLVSRQVFTGAGKMGAEAPWDDQDRHRYQLTQRADFFEAEVGLETTLK
                     RPIINTRDEPHADPEKYRRLHVIIGDANMMEVATYLKVGTAALVLKMIEDEYLPDLSL
                     AN"
BASE COUNT          839 a         1725 c         1827 g          746 t
ORIGIN      
        1 gggagccatc tcgaacccct cggcgcccgt cgcctacctc ggagtggtga cccaggacgt
       61 ggggccgagc atcgccttcc agctgaacct gccggtgcag agcggcgcgc tggtcgtgag
      121 caccacgccc gacggacccg cctcgaaggc cggcgtcgac gccggcgacg tgatcgtgtc
      181 cttcgatggg aaggacgtga agagctccca gcagctcggc tccctgatcc accagcgcca
      241 gcccggcgac caggtgccgg tggtcgtggt caagcccgat ggctcccggc agaccgtcac
      301 cgcgacgctg ggaaccagcc ccaacccgct cgcgtagcac cggggtggac cgagcgccga
      361 cctgctccga cgcccgcggg cggtgcgcta gccttgaggt ccgatgaggg tgaggcttcg
      421 taacccggac cgcgagctcg aggtggccgg ggaccgcacg gtccgtgagg tcctggcgga
      481 gctcgggatc gatccggaca cggtcctggt gatccgggat cgggacctgc tgatgcgcca
      541 ggatcgggtg gcgggtgatg atctgctgga ggtgcgtccg gtgatctcgg gagggtcgtg
      601 atgaggtgcc ggcgatgccg cggtcccgcg gtggtggaga tccgccgcca caacgcggcc
      661 ttctgccgcg actgcttcct ccggcacgtg cgcgagcagg tcaagcgggc catcgcctcc
      721 tatcacatgc tcgaggccga cgatcgggtc ctggtggccg tctcgggggg caaggattcg
      781 ctgggcctgt gggacgtgct cctggacctg gggtaccgag cctcggggct gtacctgggg
      841 cttgggatag ggacctactc ggatcggtcc ggcgacctgg cgcaccggtt ctccgccgcg
      901 cgcgaggccg agctcctgac cgtggacctg gaagccgact acgggttcga cgtgccgacc
      961 gccggcaagc ggggaggaag gtccacgtgc gccgtgtgcg gtctctccaa gcgctacgtg
     1021 ttcaaccgcg tggccctcga gcacgggttc gacgtggtcg ccaccgggca caacctcgac
     1081 gacgaggcgg cgaccctgct ggggaacacg ctgcgttggc agaccgagta catcgcgcgg
     1141 cagttcccgg ttctgccggc cgagcagggg ctggtgaaga aggtcaagcc gctgttccgg
     1201 ctctccgagc tggagacggc cgcctacgcc ttcctccggg ggatcgacta cgtggtggag
     1261 gagtgccccc tggtgggcgg caacacccag ctccggtaca aggacgccat gaaccgactg
     1321 gaggcgggct cccccggcac gaaggcccag ttcctgttcg ggtacctgga ccgggccgcc
     1381 cccctgttcg cgcgccagga cgacgccacc ctcggtccct gccaacgctg cgggcaaccc
     1441 accacgggcc ggttctgcgc cttctgccgg gcccaggctc agatcctggg gcaccggttg
     1501 ggggcacggc aggcggccgg cgacgagcat cgccaggtcg tccgcgagtc gtccgacgag
     1561 gtgctccccg ccgagatcta cggtggggca gggacctgag gtcgcccgga ggggtaccgc
     1621 ggatgggggg gatgttccag cccggggagc gcgtgctgct cgtcgacgct cggggccgtc
     1681 ggtacatggt caaccggctg cgcccggggg agagctggca ttcgcacgcc ggcgcggtgc
     1741 cgcacgacct gatcctggga tctcaggaag gtgtgcgggt gcactccacg ggggggatgg
     1801 gcttcgactg tttccgcccc cgcttggtgg acttcgtcct caagatgcct cgcggagccc
     1861 aggtcgtcta cccgaaggat ctgggcgcca tcctgatcga gggggacgtc ttcccgggga
     1921 ctcgggtcct cgaggcgggg acgggctccg gctcgctcac catcgccctg gccagagcgg
     1981 tgggggacgg gggggcagtg gtctcctacg agctgcgtga ggagttccac ctcaaagcgg
     2041 cccgcaacgt cgaagccttc ttcggctccc agccgccgtg gctggacctc cgcctcggcg
     2101 acctccgcga catctcctcc tccgagcgct tcgaccgggt cgtgctggac ctgccggagc
     2161 cctggggagt cctcgaggcg gtgttggcgg tgctggaccg ggggggcgtg ctctgcgcgt
     2221 acgtgcccac gacgatccag gcccagacca ccgtcctggc gatgggggag cgggggttcg
     2281 ggcaggtcca gaccttcgag gtcctgcacc ggccctggca cgtgacggac cggagcgtgc
     2341 ggcccgatca ccggatggtg gcccacaccg ggttcatcac ggttgggcgc ctcggagagc
     2401 gggtaccggg cgggcagggt tcatgaggac cggccggggg agaccggccc gagggtgggc
     2461 agcgcccacc tctcggcgag ggctcgcgct tcggggcgat cggggtccca gcgtatactg
     2521 gcccgaaggc gggaggtgca gcccatgccc gagtcggacc ctcgcgagca gcaggaacgc
     2581 tacgagaagc agatccacga gctccagacg caggtgcgct tcctggagga cgaggtggct
     2641 ctgctccgac gccgcctcac gaacgcccct cgacaggtga agatcctgga ggagaagctc
     2701 ctggagacca agggggacct ctccagggcc gtcacccaga acgagaagct cgccggcgcg
     2761 ctggcggccg agcgtgaccg gatcgagtcc ctgcgcgagg aggtcgagaa gctcagccag
     2821 cccccggcca ccttcggcgt ctacctgagc cgcaacgacg acgggtccgt ggacgtcttc
     2881 actgccggtc gcaagatgcg ggtcaacgtg gcgcccgagc tcgacctggt ccagctgcac
     2941 cggggggccg aggtcatcct gaacgaggcc ctgaacgtcg tcgacatcct gcgcccggac
     3001 gtgtcgggcg aggtcatgcg ggtgaaggac cgcctgggga ccgagcgcgt gatcgtggtg
     3061 gggcacgggg acgaggagct cgtggccacc ctcggccacg gcctgcacag cgagcccatc
     3121 cgggccggtg acccgctgtt ggtggatcgg cgctccaacg tggcgctgga acggttgccg
     3181 aaggaagagg tcgaagagct ggtcctggag gaggtgccgg acgtctccta cgaggacatc
     3241 ggcggcctgg agggccagat cgaggccatc cgcgacgccg tggagctgcc gttcctgtac
     3301 gcggagctgt tccacgaaca tcagctcgag cctccgaagg gcgtgctcct gtacgggccc
     3361 ccggggtgcg gcaagaccct catcgcgaag gcggtggcca agtcgctggc ggacaaggtg
     3421 cgggagcgga ccggccgccc cgatgcccac tcctacttcc tgaacatcaa ggggcccgag
     3481 ctcctgaaca agtacgtggg cgagaccgag cgacagatcc gccagatctt ccagcgggcc
     3541 aaggagcgca gcgaggaagg cctgccggtg atcgtcttct tcgacgagat ggactcgatc
     3601 ttccgcacca ggggctcggg gatctcctcc gacgtcgaga acacgatcgt cccccagctc
     3661 ctctcggagc tggacggcgt ggagaccctg aagaacgtca tcgtgatcgg ggcctccaac
     3721 cgcgaggacc tgatcgaccc ggcgatcctg cggcccgggc gcctggacgt gaagatcaag
     3781 atcgagcgcc ccagcgccgc tcaggccgcc gacatcatgg gcaagtacct gaccgccacc
     3841 gtgcccatcc atcccgacga ggtgcgtcgg gccggggacg tcacccaggc cgtcccccgg
     3901 atgatccagg gcaccgtcga ccggatgtac gcgcagaccg acgacaaccg gttcctggag
     3961 gtgacctacg cgtccggcga caaggaggtc ctgtacttca aggacttcaa ctccggggcg
     4021 atgatcgaga acatcgtccg ccgggccaag aaggaggcca tcaagcgctt cctctccacc
     4081 ggggagaagg ggatcaagac ggaggacctg ctcctggcga tccgtgacga gttcaaagag
     4141 aacgaggacc tgccgaacac caccaatccc gacgactggg cccggatctc cggcaagaag
     4201 ggggagcgga tcgtctacgt tcgcaccctg ctcggggacg gcggcgacga gggccggcag
     4261 gtcgagcggg tctcggccgg ccagtacctg tagggttcct cgatggccat cccgaaggtc
     4321 atcggcaccg agaccgagta cggcatctcg gccgtgggag cgccggactt caacccggtg
     4381 ctgtcctcct ccctgctgat cagcacctac gccggtacgc tgcgccggat ccgctgggac
     4441 tacgagcagg agtcgccgct ccgcgacgct cggggcttcg agcccgtcca gatgcgcgag
     4501 cccgtcgagg aggacctggg cctggccaac gtgatcctgc cgaacggcgc ccgctactac
     4561 gtcgaccatg cgcaccccga gtactcgacc cccgagtgcg ccgaccctcg cgagctggtg
     4621 atccacgaca aggccggcga acggatcctg gagcggtcgc tgcaggagct gcatcgcgcg
     4681 ctgccccccg gccagcggat cgcgatctac aagaacaaca cggacggcaa ggggaactcc
     4741 tacggcacgc acgagaacta cctggtggac cgggccaccc cgttcgcgaa catcgtgcgg
     4801 gacttcaccc cctacctcgt gtcccgccag gtgttcaccg gcgccggcaa gatgggcgcc
     4861 gaggctccct gggacgacca ggatcggcac cgctaccagc tgacgcagcg ggcggatttc
     4921 ttcgaggccg aggtgggcct tgagaccacc ctgaagcggc ccatcatcaa cacgagggac
     4981 gagccgcacg ccgatcccga gaagtaccga cggctccacg tgatcatcgg cgacgccaac
     5041 atgatggagg tggccaccta cctcaaggtg ggcacggcag ccctggtcct gaagatgatc
     5101 gaggacgagt acctgccaga cctgtccttg gccaacc
//
LOCUS       DASQQQ010000322         3312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_352688, whole genome shotgun sequence.
ACCESSION   DASQQQ010000322 DASQQQ010000000
VERSION     DASQQQ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352688"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VE646_12080"
     CDS             <1..192
                     /locus_tag="VE646_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR1/FUN34/YaaH family transporter"
                     /protein_id="HZD80761.1"
                     /translation="GFLGQTDSIVKAGGVIGIITAAVAWYTSAAGVVNGMAGHPVLSV
                     GGPIWKEHVQVSPVPKLAA"
     gene            complement(202..2124)
                     /locus_tag="VE646_12085"
     CDS             complement(202..2124)
                     /locus_tag="VE646_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HZD80762.1"
                     /translation="MPEPDRILAGLNEAQREAVRTVRGPVVILAGAGTGKTTTITHRI
                     AYQVATGSFPASAILAVTFTEKAARELKGRLAALGTSGAQARTFHAAALAQLSRLWPR
                     YEGEPLPQVLDSKAPLIASLANALPPPHRYLPRGELANEIEWAKNRMVPPAGYLDELE
                     ARDHEPPIPAELMLRVYEGYERRKERTRRLDFEDMLALAVRLFDRHLAAVEEVRDRFR
                     AFTVDEYQDVNALQEALLERWLGDRDDVCVVGDDHQTIYTFTGASPEHLLGFPQRFAR
                     ARVVRLEQSYRSTPQILTVANALGRHLGGFGTRLRTSLPDGPSPTVRSLSDAASEVAF
                     VVEEVCGLRERGVHLEAIAVLLRINARSEPFEEAFAAAGIPYQVRDGAFLRRPGPRAV
                     LARLRRASGPPVPAVRAATEELGFDPAAAPDQEEEATRQADLARMRVLAEEFRAAHPD
                     GELAGFVAELARRFSPEESGRGVNLLTYHRAKGLEFDAVFLPRLLDGELPYRSRRSEA
                     DPEEERRLLYVGITRARTHLFLSWPREARSAPSPFLTELGVAHPPVRHHPSRGPAAVR
                     GSGPLFERLKGWRRERAKADGVPAYVVFHDATLAAIAQQQPRSRASLACISGVGPAKL
                     ERYADEVLELVARSQV"
     gene            complement(2175..2705)
                     /locus_tag="VE646_12090"
     CDS             complement(2175..2705)
                     /locus_tag="VE646_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80763.1"
                     /translation="MLGTSIYLLVFRILHITAGVAWGGAVFLLVFFLQPASASLGPAA
                     GPFLGELLGRRKLVDAILWVAAFTVGAGLFLYWHDWQTYGSLGHFLDTGFGLSLTIGA
                     ASALVAFLIGLFGTKPNVDRLMRLAAQVAEAGGSPPAGVAAEIPAIQARLKTLARVGL
                     TLIVVAVVAMATARYW"
     gene            complement(2826..3296)
                     /locus_tag="VE646_12095"
     CDS             complement(2826..3296)
                     /locus_tag="VE646_12095"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZD80764.1"
                     /translation="MSGGAAIAPGPAEIASARDEQGRPTHQGWPPCGSRRRWMAPFVL
                     ALLSEGGAHGYALIARLQEMGVSEGEIDVGQVYRTLRCLENLGHVRSSWSDEPTGPRR
                     REYELTEAGAEALDEWAAVMAERTRLIRDFQLRYDRWKRAGSGLGPGRPRDAAS"
BASE COUNT          477 a         1224 c         1200 g          411 t
ORIGIN      
        1 ggcttcctcg ggcagacgga ctcgatcgtg aaggccggag gggtgatcgg gatcatcacc
       61 gcggccgtgg cctggtacac ctcggcggcc ggggtggtca acgggatggc cgggcacccg
      121 gtgctgagcg tgggcggacc gatctggaag gagcacgtcc aggtcagccc tgtccccaag
      181 ctggccgcgt agcggcggtc ctcagacctg ggagcgggcc accagctcga gcacctcgtc
      241 cgcgtagcgc tcgagcttgg cggggcccac cccggagatg caggcgagcg aggctcgcga
      301 acgcggctgt tgctgggcga tggcggccag ggtggcgtcg tggaacacga cgtaggcggg
      361 cacgccgtcg gccttggccc gctcccgccg ccaccccttg agccgctcga acagcggccc
      421 ggacccccgg acggcggcgg gtccgcgaga cggatggtgc cggacgggcg ggtgcgcgac
      481 cccgagctcg gtcaggaagg ggctgggcgc cgagcgggcc tcccggggcc aggacaggaa
      541 cagatgggtg cgggcccggg tgatgcccac gtacaggagc cgccgttcct cctccggatc
      601 ggcctcggac cgccgggacc ggtagggcag ctcgccgtcc agcaaccggg gcaggaagac
      661 ggcgtcgaac tcgaggccct tggctcggtg gtaggtgagt aggttcaccc cgcgtccgga
      721 ctcctccggg gagaagcggc gtgcgagctc ggccacgaac ccggcgagct cgccgtcggg
      781 atgggccgcc cggaactcct cggccagcac gcgcatccga gccaggtccg cctgacgggt
      841 ggcttcctcc tcctggtcgg gcgccgccgc cgggtcgaag cccagctcct cggtggccgc
      901 ccgcacggcc gggaccggcg gccccgacgc ccgccgcagg cgcgccagga cggcccgcgg
      961 accgggtcgg cgcaggaacg ccccgtcccg cacctggtac gggatcccgg cggcggcgaa
     1021 cgcctcctcg aacggctccg accgggcgtt gatccggagc aggacggcga tcgcctcgag
     1081 gtgcaccccg cgctccctga gcccgcagac ctcctccacc acgaaggcca cctccgaggc
     1141 cgcatcggac agggagcgga cggtgggact cgggccatcc gggagcgacg tccgcaatcg
     1201 cgtgccgaac ccacccagat gccgccccag ggcgttggcc acggtgagga tctgcggcgt
     1261 ggagcggtag ctctgctcga gccgcaccac ccgggcccgg gcgaaccgct gggggaaccc
     1321 cagcaggtgc tccggcgagg ctccggtgaa cgtgtagatc gtctggtgat cgtcccccac
     1381 cacgcacacg tcgtcgcgat cgcccagcca ccgctcgagc agggcctcct gcagcgcgtt
     1441 cacgtcctgg tactcgtcca ccgtgaaggc ccggaaccgg tcccggacct cctcgaccgc
     1501 ggccaggtgc cggtcgaaca ggcggacggc cagcgcgagc atgtcctcga agtccagacg
     1561 ccgcgtgcgc tccttccgcc gttcgtagcc ctcgtacacg cggagcatca gctcggctgg
     1621 gatcggtggc tcgtggtccc gcgcctccag ctcgtccagg tatcccgccg gggggaccat
     1681 gcggttcttc gcccattcga tctcgttggc gagctcacct cgaggcaggt accggtgcgg
     1741 cggcggcaac gcgttcgcca acgaggcgat cagcggcgcc ttcgagtcca ggacctgtgg
     1801 caagggctca ccttcgtacc tcggccacag ccgggacagc tgggccaggg cggcggcgtg
     1861 gaaggtgcgc gcttgggcgc ccgacgtccc gagcgcggcc agccgaccct tcagctcccg
     1921 ggccgccttc tcggtgaagg tcacggccag gatggccgag gcggggaagc tccccgtcgc
     1981 cacctggtag gcgatgcggt gcgtgatggt ggtggtcttg ccggtgccgg ctccggccag
     2041 gatgacgacg ggcccgcgga ccgtgcggac cgcctcccgc tgggcctcgt tcagcccggc
     2101 caggatccga tccggctccg gcatgcaacg caagctacca ggacggtccg acacgccgta
     2161 ggaaccgcgg gcgcctacca gtaccgagcg gtggccatcg cgaccaccgc caccacgatc
     2221 agcgtgagtc ccacccgggc cagggtcttc agccgggcct ggatcgccgg gatctccgcg
     2281 gccacgcccg ccggcggcga tccgcccgcc tcggccacct gcgccgccag gcgcatcagc
     2341 cggtcgacgt tgggcttggt gccgaacagt ccgatcagga aggccaccag ggcgctggcc
     2401 gcgccgatgg tcaggctgag cccgaacccc gtgtccagga agtggcccag gctcccgtac
     2461 gtctgccagt cgtgccagta gaggaacagc cccgcgccga cggtgaacgc ggcgacccac
     2521 aggatcgcat cgacgagctt gcgccggccg agcagctccc ccaggaacgg tcccgccgcc
     2581 ggcccgagcg acgccgaggc gggctgcagg aagaagacga gcaggaagac cgccccaccc
     2641 caggccacgc ccgccgtgat gtgcaggatg cggaagacga gcaggtagat gctggttccg
     2701 agcacgaacc ctccctcccg cgggctgcgg gcatcctccg ccgctccatg cccgacgtca
     2761 accgggtccg gggcccgggg cccggggcgg tcgggtgcga tcagacggtg ctgtgtcggg
     2821 aggggtcagg acgcagcgtc gcgggggcgg cccggcccga gccccgaccc ggcccgcttc
     2881 caccggtcgt agcggagctg gaaatcacgg atcaggcggg tccgctcggc catgacggcg
     2941 gcccactcgt ccagggcctc ggcgcccgcc tcggtcagct cgtactcgcg acgacggggg
     3001 ccggtgggct cgtccgacca gctggagcgg acgtggccga ggttctcgag acaacgcagc
     3061 gtccggtaca cctgacccac gtcgatctcg ccctcggaga cccccatctc ctggagcctg
     3121 gcgatcagcg cgtagccatg cgcgccgccc tcggacagca gggccagcac gaagggggcc
     3181 atccagcggc gccggctccc gcacggcggc cacccctggt gggtgggccg cccctgctca
     3241 tcacgggccg aggcgatctc cgccggaccg ggagcgatcg cggcaccccc gctcatgccg
     3301 cctcacccat cg
//
LOCUS       DASQQQ010000323         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_405590, whole genome shotgun sequence.
ACCESSION   DASQQQ010000323 DASQQQ010000000
VERSION     DASQQQ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405590"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VE646_12100"
     CDS             <1..412
                     /locus_tag="VE646_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZD80765.1"
                     /translation="LDKAVLQLFRIGFERVLGHLEGGVEAWRASGREIASYPLVGVDE
                     LARSAAEDPERIMDVRQRDEWEAGHLPGTRHLFVGDLPGRLGELPTDVPLTVACASGY
                     RASMAASLLARAGNPVRLVAEGGVPDALGPRAGA"
     gene            complement(476..766)
                     /locus_tag="VE646_12105"
     CDS             complement(476..766)
                     /locus_tag="VE646_12105"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZD80766.1"
                     /translation="MAVLGCGIDGAYPAGSRSLLQRIERTGAIVSEYPPGVPPEPQRF
                     PARNRIIAGLCSALVVVQGRERSGSLISARHALEHGRDVYAVPGAPCCSSWR"
     gene            complement(834..1286)
                     /locus_tag="VE646_12110"
                     /pseudo
     CDS             complement(834..1286)
                     /locus_tag="VE646_12110"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
     gene            complement(1304..1855)
                     /locus_tag="VE646_12115"
     CDS             complement(1304..1855)
                     /locus_tag="VE646_12115"
                     /inference="COORDINATES: protein motif:HMM:NF013260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZD80767.1"
                     /translation="MTRVWGAVEFPARFTLIAGANPCPCGYDGDSVRQCECLPHRVRL
                     YRQKLSGPLLDRIDLRLAVPRLSRRELLEEAPGEPSAAVRDRVEEARERQGRRYAGPP
                     LSLQRPGARAARAAHGEGVGPGMRPAGPGGGDVRPTGGGFDRAIKVARTIADLAGAEL
                     VGAVSEALSYRMDLAGEGLARAG"
     gene            complement(1976..>2339)
                     /locus_tag="VE646_12120"
     CDS             complement(1976..>2339)
                     /locus_tag="VE646_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80768.1"
                     /translation="WRMRVRLFAATVLLLIGVGSLTGCSLAPGREPVFHTYTYTGTVT
                     AVCSHTTQGIPDCFALTPDPGTAHRDGYYAGAGAVSFGPPAKRDPIPRIGDHVTVTVN
                     QAVNQAGTVTRVSRAPTA"
BASE COUNT          317 a          893 c          832 g          297 t
ORIGIN      
        1 cctggacaag gccgtcctgc aactgttccg gatcggcttc gagcgggtcc tgggtcacct
       61 ggagggcggc gtggaggcct ggcgggcctc gggacgggag atcgcctcgt acccgctggt
      121 cggggtggac gagctcgcgc gctcggcggc ggaggacccc gagcggatca tggacgtccg
      181 gcagcgggac gagtgggagg ccggtcacct gcccgggacg cgccacctgt tcgtgggcga
      241 ccttccggga cggctgggcg agcttcccac cgacgtgccg ctcaccgtgg cctgcgccag
      301 cggctaccgg gcctcgatgg ccgcgagcct gctggcgcgg gcggggaacc cggtccgact
      361 ggtggccgag ggcggcgtgc ccgacgcgct cggccctcgg gccggcgcgt gagccctcag
      421 gcatctgccg gcaggcgccg ctggtagcgt ccgccggcgc tccggaccag ccccttcatc
      481 tccagctgga acagcagggg gcgccgggca cggcgtacac gtcccgtccg tgctccagcg
      541 cgtggcgggc ggagatcagc gacccgctgc gctcccgccc ctgaaccacc acgagcgccg
      601 agcacagccc cgcgatgatc cggttgcggg ccgggaagcg ctggggctcg ggcgggaccc
      661 cgggtgggta ttcgctcacg atcgctccgg tccgttcgat ccgctgcagg agcgaccggc
      721 tccccgcggg gtacgcgccg tcgatcccgc accccagcac cgccaccgtg cgccccccgc
      781 ttccaggccc cgtcgtgggc cgctgcgtcg atcccgcgcg ccgcgccgct cacgaccacg
      841 gccccggccg cggccagcgc gtgaccgaga ccctgggcca gctcgcgccc gaagggggag
      901 cagctccggg cccccacgac ggccaccgtc cccggctggg cgggcagctc cccgcctcgc
      961 acgtagagcg cggcgggagg atcggccagg tactcgagct gggccgggta gcccgccgag
     1021 cccccacagg atcgcccgcg ccccgcatgc ctcggctcgt gccaggatct ccgcgggccg
     1081 gagcgtccgg gcgtgcttct ggtcggcctc gctgccggcc cgtccctggc ggatggccgc
     1141 cagggacccc accgcgctcc cctcggctcg cgccagatcc aggagcttgg ccggcgtgat
     1201 gccacccagc gccgacagca ccaccgccgc ctccagttcc cgccgtccgt gaccgaagcc
     1261 ttcgggccat ccctgggaga cctcacccgg caccggccac ccgtcagccg gcacgggcca
     1321 gcccctcgcc ggccagatcc atgcggtacg agagcgcctc cgagacggcg ccgaccagct
     1381 cggcccccgc cagatcggcg atggtcctgg ccaccttgat ggcgcggtcg aagccccccc
     1441 ccgtaggccg aacgtctcca ccgcccgggc cagcagggcg catgccgggt ccgacacctt
     1501 ccccgtgcgc cgcgcgagcg gcccgggcac ccgggcgttg cagggatagg ggggggcctg
     1561 cgtagcgacg accctgacgt tcgcgagcct cctccacccg atctcggacc gcggccgagg
     1621 gctcgcccgg agcttcctcc aagagctccc gccgcgacag gcgcggcacc gccaaccgca
     1681 gatcgatgcg atccaggagc ggacccgaga gcttctgccg gtacagccgg acccggtgcg
     1741 gcaggcactc gcactgacgg accgaatcgc cgtcgtagcc gcaggggcac gggttcgccc
     1801 ccgcgatcag cgtgaagcgg gccgggaact ccaccgcacc ccacacgcgg gtcaccccgt
     1861 ccgtcctcga ggggctggcg gagcgcctcg agggcatctc gccggaactc cgtgagctcg
     1921 tccaggaaga gcacaccgtg atctgcgagc gtctgaggat gtcccctgag ccgctctatg
     1981 cggtgggcgc acgcgatact cgagtgacgg tacccgcctg gttcaccgcc tggttcaccg
     2041 tgacggtcac gtgatcgcca atccgtggga tcggatctct cttcgccggt gggccgaagc
     2101 taaccgcccc agcgccagcg tagtaaccgt cgcgatgggc cgtgcccggg tctggtgtga
     2161 gggcgaagca gtcggggatg ccctgagtcg tatgcgagca gacggcggtg accgtccccg
     2221 tgtacgtgta ggtgtgaaag acgggctcgc gcccgggcgc gagcgagcag ccggtgagcg
     2281 agcctacacc gatgaggaga agaaccgtcg cggcgaacag ccggactctc attcgccat
//
LOCUS       DASQQQ010000324         5800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_376875, whole genome shotgun sequence.
ACCESSION   DASQQQ010000324 DASQQQ010000000
VERSION     DASQQQ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376875"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VE646_12125"
     CDS             complement(<1..419)
                     /locus_tag="VE646_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZD80769.1"
                     /translation="MSRSSQLCDRPASELAERLRVGEIRSTEIAGSCLERIAALEPRL
                     GAFLLPTEELARRQAAEVEERIASGSAGAVTGIPVALKDVLSTRGVRTTCGSKILETY
                     VPPYDCTPWARLAEAGSVLLGKTNCDEFAMGSSNENSA"
     gene            complement(416..706)
                     /gene="gatC"
                     /locus_tag="VE646_12130"
     CDS             complement(416..706)
                     /gene="gatC"
                     /locus_tag="VE646_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZD80770.1"
                     /translation="MPVEIDIDHVARLARLDLTEEERERLRAQLGAIIEHAGKVGEVA
                     TEDVPPTAWAIPRANVLRPDEPTPSLPPEDVLANAPEREDDRFRVPRIVEVE"
     gene            complement(755..2254)
                     /locus_tag="VE646_12135"
     CDS             complement(755..2254)
                     /locus_tag="VE646_12135"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HZD80771.1"
                     /translation="MSGRTSNSEIRAARELLRSLSSARQTYTLYPPGHPLRVEAAREL
                     TRLMQELRRIHGGTPALFQTDGNLYLGRTLLAWESLTLHRLGESLDEAGVASIEFGTA
                     VSEEDADAMVRLLMGEVQAKDELASITLNRAGPGLLEGPEGGFAELLRTYAVGLEVIR
                     QTAARLLAGRPADMDATVRLTEHLASLISGDPAQALLLTTVKSYDEYTYHHMVNVCIL
                     SLAIAQAAGLPHEQAIALGIGGLLHDVGKVKVPQEVLQYAGSLDEEQWRLIQRHPVDG
                     AALVLVTSRDPLHPAMAVVLEHHAAYDRSGYPTLSGGRPPSAAARIVSVADSFDALTS
                     KRSYRKPEERRQALNLIQAGAGRSFDPRVVRTFVRLMGLFPVGSLVQLSNGSVGVVVR
                     NHDRLLARPTVRLVLDEKGNQVDAEELDLAQESRTGGDRASVVRSIDPADVGLDMLTL
                     LASGRLDVPPPREAGPGLVHEPAPVENAPPGYVDVHHELAAEGPDHGPA"
     gene            complement(2251..4179)
                     /locus_tag="VE646_12140"
     CDS             complement(2251..4179)
                     /locus_tag="VE646_12140"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZD80772.1"
                     /translation="MLRQLWLGLSTYRLFPEDMSRPTFVTAAERVAEAAAAALSMGPV
                     EVGIRGSLFVLDGEQLPEDEAAAKLGLACFERRVELLALHGVPDPEDLAALYKVLTTA
                     SEDLREAGGAAEVLYAAGVTTMALSAVGPAPVTGADHVPEELAEAPVPASKVPDIDVL
                     ASELMIEDLHGSPADQARTLLARLKSFTGGLALEPGQGIDLQSAVHDVITQLPDEIRR
                     ALVEELVDTVKDDPVAQRLISTMSNAELTRALVDLGRTGPRDPVVMARQLAEAGVRHL
                     DLIDLTTALQKGREEAGTIIAGLEQLGLNLDEEPPQAGGSVGEVLAGYLMGTRADDVR
                     MIQAAVPHTERQRRDAALMALRDYLLIETDTELVGEALHVWSEEVERALGARDVELAA
                     ALVPPIREALAGSSDPERATLFENAVKHALVRDLVLDMAATVEASDGVGVQALLAPFG
                     DAGVEALLDLLADEEDRDRRAHLLGLLRRVASGHPGPVVARLRDPRWFVVRNAANLLG
                     ATGGPVVLEQLAGATRHEVPEVRREAVKALMVAGGAAAVPHLTGAALEGPEDVRSSAV
                     VALGAVLAPQAAEALATVARTCRNRSLAIRAVDELAGHPEGRGALRGVATGRGRPRAS
                     WRVRRYARKVQGKEALGS"
     gene            complement(4339..>5800)
                     /gene="ligA"
                     /locus_tag="VE646_12145"
     CDS             complement(4339..>5800)
                     /gene="ligA"
                     /locus_tag="VE646_12145"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZD80773.1"
                     /translation="NLNRELTEAGQRAFANPRNAAAGSLRQKEARVTASRPLSLWVHS
                     FGFAQAIRFDSHSGFLSWCRDAGLPVASTSRVEEDLQGVRAYLDQWQEHRHSVDWEID
                     GVVVKVDQIPLQEELGATSHAPRWAVAYKFPPEERTTLLRRIDVHTGRTGIVTPFAVL
                     EPVQVSGVTVGTATLHNEDEVRRKDVREGDTVIVRRAGDVIPEVVGPVPAKRPKAARP
                     WRFPKRCPSCGTELVRREGEAYRRCPNRRGCPSQTVEWLFAFAGRGAMDIEGLGYQTL
                     LALLDRALIEDPGDIYSLTEEQVGALEGFAERSTRNLLDGIAASKDRPLWRLLVGLNI
                     PHVGTHVAQVLARAFRSIDALAGAGEEEIDAVPEIGPEIAGSVRAWFDETENLALVDK
                     LRQRGVRVRDPEPERPVEGPLSGSTIVLTGTLGGMSRDEAARAAQEAGARVASSVSKR
                     TDLVVVGDNPGSKYDRAVELGVETIDEQEFRRRLRR"
BASE COUNT          788 a         2167 c         2030 g          815 t
ORIGIN      
        1 gccgagttct cgttggagga gcccatcgcg aactcgtcgc agttcgtctt gcccagcagg
       61 accgaccccg cctcggccag ccgggcccac ggggtgcagt cgtagggcgg cacgtacgtc
      121 tcgaggatct tcgacccgca ggtggttcga accccgcggg tgctcagcac gtccttcagg
      181 gcgacgggga tgccggtaac ggctccggcg gaacccgagg cgatccgctc ctcgacctcg
      241 gccgcctgcc ggcgggcgag ctcctccgtg ggcagcagga aggcgccgag gcgcggctcc
      301 agggcggcga tccgctccag acaggatccg gcgatctcgg tcgaacggat ctcgccgacg
      361 cgcagccgct ccgcgagctc gctcgcgggt cgatcgcaga gctgggagga gcggctcact
      421 ccacctccac gatgcgcggg acccggaagc ggtcgtcttc tcgctcgggg gcgttggcca
      481 gcacgtcctc cggtggcagg gacggcgttg gctcgtcggg tcgcaagacg ttcgcccggg
      541 ggatcgccca ggcggtgggg gggacgtcct ccgtggccac ctcgcccacc ttcccggcgt
      601 gctcgatgat cgccccgagc tgggcccgca gccgctcccg ctcctcctcg gtcaggtcca
      661 gacgagcgag ccgggccacg tgatcgatgt cgatctccac cggcatggcc ggaagtgtag
      721 tggtcgcgtg gccggccggc cccggcggtc ggcctcaggc gggtccgtga tccgggccct
      781 cggcggcgag ctcgtggtgg acgtcgacgt agccgggggg agcgttctcg acgggggcag
      841 gctcgtggac cagtcccggc ccggcctccc gcggaggtgg cacgtccagc cggcccgatg
      901 ccaagagcgt gagcatgtcg agcccgacgt cggcagggtc gatgctccgg accaccgagg
      961 cccggtcgcc tccggtgcgg ctctcctggg ccaggtccag ctcctcggcg tcgacctggt
     1021 tccccttctc gtccagcacc agccggacgg tgggacgtgc cagcaaacgg tcgtggttgc
     1081 ggaccacgac ccccaccgag ccgttcgaga gctgcaccag cgatcccacc gggaacagcc
     1141 ccatcaggcg cacgaacgtt cgcaccacgc ggggatcgaa ggagcgcccg gcaccggcct
     1201 ggatcaggtt cagggcctgg cggcgctctt ccggcttgcg gtaagaccgc ttgctggtga
     1261 gggcgtcgaa gctgtcggcg accgagacga tccgggccgc cgccgagggg ggccggccac
     1321 cggacagcgt tgggtatccc gaccggtcgt aggcggcgtg gtgctcgagc accacggcca
     1381 tcgccgggtg gaggggatcg cgagaggtca ccaggacgag ggccgcgccg tccacgggat
     1441 gccgctggat cagccgccac tgctcctcgt ccaacgatcc cgcgtactgg aggacctcct
     1501 gcgggacctt caccttgccc acgtcgtgga gcagcccccc gatgccgagc gcgatcgcct
     1561 gctcgtgcgg aaggccggcc gcctgagcga tggccagcga caggatgcac acgttcacca
     1621 tgtggtggta cgtgtattcg tcgtaggact tcaccgtggt gagcagcagc gcctgcgccg
     1681 gatccccgga gatgagcgac gccaggtgct cggtcagacg caccgtcgca tccatgtcgg
     1741 cggggcggcc ggccagcagg cgggcggcgg tctggcggat gacctccagc cccaccgcgt
     1801 aggtgcggag cagctcggcg aagccgccct cggggccctc caggagtccc ggaccggccc
     1861 ggttgagggt gatcgaggcg agctcgtcct tggcctggac ctcgcccatc aggagccgga
     1921 ccatggcgtc ggcgtcctcc tccgagacag ccgtcccgaa ctcgatcgag gccacccccg
     1981 cttcgtcgag cgattccccc agccggtgca gggtgagcga ttcccaggcc aacagggtgc
     2041 ggcccaggta gaggttcccg tcggtctgga acagcgccgg ggtgccaccg tggatccggc
     2101 gaagctcctg catcagcctg gtgagctcgc gagccgcctc cacacgcagg ggatgacccg
     2161 gcgggtacag cgtgtaggtc tgccgggccg acgagagcga ccgcagcagc tcacgggcgg
     2221 ccctgatctc cgagttcgac gttctgccgc tcacgatcca agcgcctcct tgccctgcac
     2281 cttgcgcgcg taccgcctga cgcgccacga cgcccgtggc cgcccccgac cggtcgccac
     2341 cccccgcagc gctccgcgac cctccgggtg accggcgagc tcgtccaccg cgcggatcgc
     2401 caaggatcgg ttccggcagg tgcgcgccac cgtggcgagc gcctcggccg cctgcggcgc
     2461 caggacggcc ccgagggcca ccacggctga gctgcggacg tcctcgggcc cctccagcgc
     2521 ggctcccgtg aggtggggga ccgccgcggc ccctccggcg accatcaggg ccttgacggc
     2581 ctctcggcgc acctccggaa cctcgtgccg ggtggcgccg gccagctgtt ccaggacgac
     2641 cggccccccg gtggcgccga gcaggttcgc cgcgttgcgg accacgaacc accggggatc
     2701 gcgcaggcgg gccaccaccg gtccggggtg cccggaggcg acccggcgga gcagaccgag
     2761 cagatgggcc cgccggtccc gatcctcctc gtcggccagc aggtccagca gggcctccac
     2821 accggcgtcg ccgaaaggcg ccagcagggc ctgcacaccg acgccgtccg acgcctccac
     2881 ggtggccgcc atgtcgagca ccaggtcgcg gaccaacgca tgcttgaccg cgttctcgaa
     2941 cagcgtggcc cgctcgggat cggacgaacc cgccagggcc tcccggatcg gtgggacgag
     3001 ggcggccgcc agctccacgt cccgagcccc cagagcacgc tcaacctcct ccgaccacac
     3061 gtgcagcgcc tcgcccacca gctcggtgtc ggtctcgatc aacaggtagt cgcggagcgc
     3121 catcagggcg gcgtcgcggc gctgtcgctc ggtgtggggc acggcggcct ggatcatccg
     3181 gacgtcgtcg gccctggtgc ccatcaggta cccggccagt acctccccca ccgagccgcc
     3241 tgcctgcggg ggctcctcgt ccaggttcag cccgagctgc tcgaggccgg cgatgatcgt
     3301 tcccgcctcc tcccggccct tctgcaacgc ggtggtcagg tcgatcaggt cgagatggcg
     3361 gacccccgcc tcggccagct ggcgggccat cacgacgggg tcgcgcgggc cggtgcggcc
     3421 cagatcgacc agggcgcggg tgagctccgc gttgctcatc gtgctgatca accgctgcgc
     3481 gacggggtcg tccttcaccg tgtccaccag ctcctccacc agcgcgcggc ggatctcgtc
     3541 ggggagctgg gtgatgacgt cgtgcaccgc cgattggagg tcgatgccct gcccgggctc
     3601 gagcgccagt ccgccggtga agctcttcag ccgagccagg agggtccggg cctgatcggc
     3661 cggtgagccg tgcaggtcct cgatcatcag ctcggaggcc aggacgtcga tgtcgggcac
     3721 cttgctcgcc gggaccggcg cttcggccag ctcctcgggc acatggtcgg cgccggtcac
     3781 cggcgcgggg cccaccgccg agagggccat cgtggtgacg cccgccgcgt acaggacctc
     3841 ggcggcgccg cccgcctccc ggaggtcctc ggacgccgtg gtcagcacct tgtacagggc
     3901 ggccaggtcc tcggggtcgg gcaccccgtg cagggccagg agctccaccc gccgctcgaa
     3961 gcaagccagg ccgagtttgg ccgccgcttc gtcctcgggg agctgctcac cgtccagcac
     4021 gaacagggat cctcggatcc cgacctcgac cggacccatc gacagcgccg cggcggcggc
     4081 ctccgccacc cgttcggccg cggtcacgaa cgtcggacgg ctcatgtcct cggggaacaa
     4141 ccggtacgtg gacagcccga gccacagctg ccggagcacc gctcctacgg cacgggctcg
     4201 ctcctcccgc cctgacacct cgcctccgtc cgacgaccta gtccgtccct cccgttcatc
     4261 ggcaaggagc cgggtgaggc tgagagggga ccttcggggg gtcggccggc gggcgtcggc
     4321 ggaggccggt ccccccgttc accggcggag ccggcgacgg aactcctgct cgtcgatcgt
     4381 ctccaccccg agctccacgg cccggtcgta cttcgagccg gggttgtccc cgacgaccac
     4441 cagatcggtc ctcttcgaca cgctggaggc cacccgggcg cctgcctcct gcgcggctcg
     4501 ggccgcctcg tcccgcgaca tgccccccag cgtcccggtg agcacgatcg tgctgcccga
     4561 caggggaccc tccaccggcc gttccggctc cgggtcgcgc acccggaccc cgcgttgacg
     4621 gagcttgtcg accagggcca ggttctcggt ctcgtcgaac cacgctcgca cgcttccggc
     4681 gatctcgggg ccgatctcgg gcaccgcgtc gatctcctcc tcgccggcgc ccgccaacgc
     4741 gtcgatcgac cggaaggccc gagccaggac ctgcgccacg tgggtgccca cgtgcgggat
     4801 gttcagaccc accagcagcc gccacagcgg gcggtccttc gaggcggcga tcccgtccag
     4861 gaggttcctc gtggagcgct ccgcgaatcc ctcgagggct ccgacctgct cctcggtgag
     4921 cgagtagatg tccccggggt cctcgatcag cgctcggtcc agcagggcca agagggtctg
     4981 atagccgagg ccctcgatgt ccatggcccc ccggcccgcg aaggcgaaca gccactccac
     5041 ggtctgggat gggcatcctc gccggttggg acagcgccgg taggcctcac cctcccggcg
     5101 cacgagctcg gtcccgcagg acggacagcg cttcgggaac cgccagggcc gggccgcctt
     5161 ggggcgcttg gccgggaccg ggcccacgac ctcggggatc acgtcgccgg cccgccgcac
     5221 gatcacggta tccccctccc ggacgtcctt gcgccggacc tcgtcctcgt tgtgcagcgt
     5281 ggcggtaccg accgtgacgc cgctcacctg cacgggctcc agaacggcga agggcgtcac
     5341 gatgccggtg cgaccggtgt gcacgtcgat gcggcggagc agcgtggtgc gttcctcggg
     5401 cgggaacttg tacgccacgg cccagcgcgg cgcgtggcta gtggccccca gctcctcctg
     5461 gagcgggatc tggtcgacct tgaccaccac gccgtcgatc tcccagtcca cgctgtggcg
     5521 gtgctcctgc cactgatcca ggtaggcgcg gacgccctgt aggtcctcct ccacccggct
     5581 ggtagacgcc accggcaggc cagcgtcccg gcaccaggaa aggaagcccg agtgcgagtc
     5641 gaaccggatg gcctgcgcga acccgaagct gtggacccac agggacagcg gccgtgaggc
     5701 ggtgacccga gcctccttct gccgcaggct gccggcggcc gcgttccgag ggttggcgaa
     5761 ggcccgctgg cccgcttcgg tgagctcccg gttgaggttg
//
LOCUS       DASQQQ010000325         4058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_323632, whole genome shotgun sequence.
ACCESSION   DASQQQ010000325 DASQQQ010000000
VERSION     DASQQQ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323632"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VE646_12150"
     CDS             <1..208
                     /locus_tag="VE646_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80774.1"
                     /translation="IFYLTETVHAAHVVVGVLLLGWLLWSTRNITAPGRTSTVQIIGL
                     YWYFLLIVAIAILLSFYLHPRFWS"
     gene            205..645
                     /locus_tag="VE646_12155"
     CDS             205..645
                     /locus_tag="VE646_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80775.1"
                     /translation="MSLAERLRREGHPESLRLLTRQTTAVSVAIWVGILGAVLLLGWT
                     VVLGDAMFELGCSPGSSAGGTLVFGLITWAHILSASAAFLTLCCLVASLWAFFRLRRM
                     EEDLRVERARVLSIAAIGISAFALLMAVYSLLPPFLMSACSTSP"
     gene            642..1490
                     /locus_tag="VE646_12160"
     CDS             642..1490
                     /locus_tag="VE646_12160"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HZD80776.1"
                     /translation="MIRWLRDPVLEPGVTALVALAAVVYLGGARRLRRRSSGRLVLPS
                     WRVGAFVGGLVVVLEAVASPLDDAATTSLTAHMIQHLLLLVVAPVLLVLGSPARPFLT
                     ALGPSIPLRRLRWTRGIWRELTRPLPVFLLYAASLLAWHVPGPYDAAVHHQALHALEH
                     LTFLGTAVLLWMVLLRERPQVPAGMAIGLAMATWVVTASLGALLTFAGAPLYPVYAHA
                     TSAWGLSPLRDQRMAGLIMWAPTGLAYIAAAGWRLLRLLAEPDAEGTPPARRTAGTLA
                     GTKGGA"
     gene            1487..1891
                     /locus_tag="VE646_12165"
     CDS             1487..1891
                     /locus_tag="VE646_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZD80777.1"
                     /translation="MSRLGRGTLTILTVVGLAAVSAACGPGTSNRLPPPQVPGGQPNR
                     GVTLIEHYGCGACHTIPGIRGADANVGPPLVGIGDRRYIAGVLVNNADNMVRWLMDPP
                     AIVSGVDMPNMGISREDAEDIAAYLYTLRGGG"
     gene            1884..2807
                     /locus_tag="VE646_12170"
     CDS             1884..2807
                     /locus_tag="VE646_12170"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZD80778.1"
                     /translation="MVDLGRQGRVRRFRWVLASVVAGLAAVPLLFLVSAGAQTSPPPN
                     PSPATPTPSIPLVPQGGQQIPTPLPSGAAVPPGGGEQIYREDCAFCHGDQAQGTELAP
                     SLQGVGAASVDFMVSTGRMPITRPERYPQRKAVLYSPAQIDALVSYVSGIIGEGPAIP
                     SVDVSAGDLHRGYELFLENCAACHSATGVGEDLTTGVSAPNLYDATATQVGEAMRIGG
                     AGLRSGDMPRFSEATFDEHDLDSIARYIGYLQQERNPGGLALYRIGAVAEGFVAWVFA
                     IGVLLLFIRWIGERAGNVEGKELKERKEERE"
     gene            2804..3688
                     /locus_tag="VE646_12175"
     CDS             2804..3688
                     /locus_tag="VE646_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HZD80779.1"
                     /translation="MRLLGDPKRARAAQVAFLVSAAGACGLAAIYVLGGNPQIEGAML
                     GVMFGALSVGLVLWAKAEMRDEGRFVEHRFPQPTEEYMREREAMIDDVGRIKQTVPRR
                     TFLLSLMAAAGAIGAVILFPLRSLGVKPDGRLFHTRWFKGSRLMTYDHEPVRVEDLVD
                     GEAITVFPEKGVGFTDSAAWLIRVEPDRLELGARSDWAHDGYVLYSKMCTHAGCPVGL
                     YRRSEHMLLCPCHQSQFDVLRGAMAVAGPAPRGLPQIPLGQDGEGYLVAESDFDEPVG
                     PGFWQVQKGIRPGEPGAP"
     gene            3685..>4058
                     /locus_tag="VE646_12180"
     CDS             3685..>4058
                     /locus_tag="VE646_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HZD80780.1"
                     /translation="MIDRMLRWFDERVGGASFIRLALNKIFPDHWSFMLGELAMYLLI
                     ILIATGTYLALFYVPTRHEVVYWGNFAPMTGHETSAAFGSVLRISFDVRAGLVIRQMH
                     HWAADLFLAAIVVHLCRIFFTGA"
BASE COUNT          486 a         1416 c         1497 g          659 t
ORIGIN      
        1 gatcttctac ctgaccgaga ccgtgcacgc cgcccacgtg gtggtcgggg tcctcctgct
       61 gggctggctg ctgtggagca cccggaacat caccgcaccc ggtcgcacct ccacggtcca
      121 gatcatcggg ctgtactggt acttcctgct gatcgtggcc atcgcgatcc tgctgtcctt
      181 ctacctgcat ccgaggttct ggtcgtgagc ctggccgagc gccttcgccg cgaggggcac
      241 ccggagagcc tccgcctgct cacccggcag accaccgccg tctcggtggc catctgggtg
      301 gggatcctgg gcgcggtgct gctcctgggc tggacggtgg tcctgggtga cgcgatgttc
      361 gagctcgggt gctcgccggg ctccagcgcg ggaggcacgc tcgtcttcgg cctgatcacc
      421 tgggcgcaca tcctgtccgc ctccgcggcc ttcctcaccc tgtgctgcct ggtggcctcg
      481 ttgtgggcct tcttccgact ccggcggatg gaggaggacc tgcgcgtgga gcgggcccgg
      541 gtcctgtcga tcgccgcgat cgggatctcg gccttcgccc tgctgatggc ggtgtactcg
      601 ctgctccccc cgttcctgat gtcggcctgc agcacgtctc catgatccgc tggctccgcg
      661 acccggtgct cgagcccggg gtcaccgcgc tggtggccct ggccgccgtc gtgtacctgg
      721 gcggcgcgcg caggctgcgc cgccggtcca gcggccggtt ggtgctgccc tcctggcggg
      781 tgggagcctt cgtcggcggc ctggtcgtgg tgctcgaagc ggtggcgtcc ccgctggacg
      841 acgcggccac gacctcgctc accgcgcaca tgatccagca cctgctgctc ctggtggtcg
      901 ccccggtgct gctggtgctt gggagccccg cgcgcccgtt cctgacggcc ctgggtccca
      961 gcatccccct ccgccgcctt cgctggaccc gggggatctg gcgggagctg acccgccccc
     1021 tgcccgtgtt cctcctgtac gccgccagcc tgctggcgtg gcacgtcccg gggccgtacg
     1081 acgcggccgt ccaccaccag gcgctgcacg ccctggagca cctcacgttc ctggggaccg
     1141 ccgtgctgct gtggatggtg cttctccgcg agcggcccca ggtcccggcc gggatggcga
     1201 tcgggttggc catggccacg tgggtggtga ccgcatccct cggcgcgctg ctgacgttcg
     1261 ccggcgctcc cctgtacccg gtgtacgcgc acgccacgtc ggcctggggg ctctcgcccc
     1321 tgcgggacca gcggatggcg gggctcatca tgtgggcccc caccggcctg gcctacatcg
     1381 cggccgcggg atggaggctg ctgcgcctct tggcggaacc ggacgcggag gggacccctc
     1441 ctgcccgacg caccgcgggg accctggcgg ggacgaaagg aggcgcgtga gccgactggg
     1501 gcggggaacg ctcacgatcc tcaccgtcgt ggggctcgcc gcggtctcgg cggcgtgcgg
     1561 gccgggcacc tcgaaccggc tgccgccccc gcaggtcccg ggggggcagc cgaaccgcgg
     1621 ggtcacgctg atcgagcact acgggtgcgg ggcctgccac acgatcccgg gcatccgagg
     1681 cgccgacgcg aacgtggggc cgcccctggt ggggatcggc gaccgtcggt acatcgcggg
     1741 cgtgctggtc aacaacgcgg acaacatggt gcgctggctg atggaccccc ccgcgatcgt
     1801 gtccggggtc gacatgccga acatggggat ctcccgggag gatgccgagg acatcgccgc
     1861 ctacctgtat acgctccggg gaggtggttg acctgggccg ccagggacgg gtccgacggt
     1921 tcaggtgggt gctggccagc gtggtggccg ggctcgccgc cgttcccctc ctgttcctgg
     1981 tctcggcggg ggcgcagacc tcgcccccgc ccaacccgtc cccggccacc ccgacgccca
     2041 gcatccccct cgtcccccag ggcgggcagc agatcccgac cccgctgcca tcgggggcgg
     2101 cggtgccccc cggggggggt gagcagatct accgcgagga ctgcgccttc tgccacggcg
     2161 accaggccca ggggacggag ctcgcgccgt cgctgcaagg ggtcggcgcg gcctcggtcg
     2221 acttcatggt ctccaccggc cggatgccga tcacgcggcc cgagcggtac ccgcagcgca
     2281 aggccgtcct gtactcgccc gcccagatcg acgccctggt gagctacgtg agcgggatca
     2341 tcggcgaggg gccggcgatc ccctcggtgg acgtgtcggc gggggacctg caccgggggt
     2401 acgagctgtt cctcgagaac tgcgcggcgt gtcactcggc taccggtgtg ggggaggatc
     2461 tgaccaccgg ggtgtcggca ccgaacctct acgacgccac cgccacccag gtgggcgagg
     2521 cgatgcggat cggcggggcg ggcctgcgct cgggcgacat gccccggttc agcgaggcca
     2581 cgttcgacga gcacgacctc gactcgatcg cccggtacat cgggtacctg cagcaggagc
     2641 gcaaccccgg cgggctggcc ctgtaccgga tcggggccgt ggcggagggc ttcgtcgcgt
     2701 gggtgttcgc gatcggcgtg ctcctgctgt tcatccgctg gatcggggag cgggcgggga
     2761 acgtcgaggg caaggagctg aaggagcgga aggaggagcg cgagtgaggc tcttgggcga
     2821 tccgaagcgg gcccgggccg cccaggtggc cttcctggtg tcggcggccg gcgcgtgcgg
     2881 cctggccgcg atctacgtgc tgggcggcaa cccccagatc gagggcgcga tgctgggggt
     2941 gatgttcggg gccctgtcgg tgggcctggt gctgtgggcc aaggccgaga tgcgcgacga
     3001 gggccggttc gtggagcacc gcttcccgca gcccaccgag gagtacatgc gggagcggga
     3061 ggccatgatc gacgacgtgg ggcgcatcaa gcagaccgtg ccccggcgga cgttcctgct
     3121 gtcgctcatg gccgctgcgg gcgcgatcgg cgccgtgatc ctgttcccgc tccggtccct
     3181 gggcgtcaag cccgacggac ggctgttcca cacccgctgg tttaagggct cgcgattgat
     3241 gacctacgac cacgagccgg tgagggtcga ggacctggtc gacggggagg ccatcacggt
     3301 cttcccggag aagggggtgg ggttcacgga ctcggcggcc tggctgatcc gggtggagcc
     3361 cgaccggttg gagctcggcg ctcgcagcga ctgggcccat gacggttacg tcctgtactc
     3421 gaagatgtgc acccacgccg ggtgtccggt gggcctgtac cggcggtccg agcacatgct
     3481 gctctgcccg tgccaccagt cgcagttcga cgtgctccgg ggggcgatgg cggtggccgg
     3541 gccggcgccc cgcggactgc cgcagatccc cctcggccag gacggcgagg ggtacctggt
     3601 ggccgagagc gacttcgacg agccggtggg acccgggttc tggcaggtcc aaaaggggat
     3661 ccggccgggg gagccggggg ccccgtgatc gatcggatgc tccggtggtt cgacgagcgc
     3721 gtcgggggcg cctcgttcat ccgcctggcg ctcaacaaga tcttccccga ccactggagc
     3781 ttcatgctgg gcgagctggc catgtacctg ctcatcatcc tgatcgccac cgggacctac
     3841 ctcgcgctct tctacgtgcc cacccgacac gaggtcgtgt actgggggaa cttcgccccc
     3901 atgaccggcc acgagacctc ggcggcgttc ggctcggtgc tccggatcag cttcgacgtc
     3961 cgcgccgggc tggtgatccg gcagatgcat cactgggcgg cggacctgtt cctggcggcg
     4021 atcgtggtgc acctgtgccg gatcttcttc accggcgc
//
LOCUS       DASQQQ010000326        19028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_134364, whole genome shotgun sequence.
ACCESSION   DASQQQ010000326 DASQQQ010000000
VERSION     DASQQQ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 19028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19028
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134364"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VE646_12185"
     CDS             <1..247
                     /locus_tag="VE646_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80781.1"
                     /translation="TAPASATTTQGEAPAEAEAEAPAAKRSTGKRSTAKKTTAKKTAA
                     KKTAAKKTTAKKTAAKTSAAKRSAAKKSTARRAKKEE"
     gene            251..643
                     /gene="rpsI"
                     /locus_tag="VE646_12190"
     CDS             251..643
                     /gene="rpsI"
                     /locus_tag="VE646_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015746498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HZD80782.1"
                     /translation="MAQAVNTSTGRRKEAICRVRLVAGSGSYHLNGRPLEDYFPTRAH
                     RMIVQSPLKLVGRDKDYDVLARIEGGGVAGQAGALRLGIARALIDLDPEFRGVLKKEG
                     MLSRDAREKERRKYGLKKARKAPQYSKR"
     gene            complement(684..962)
                     /locus_tag="VE646_12195"
     CDS             complement(684..962)
                     /locus_tag="VE646_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80783.1"
                     /translation="MGDEDFVRVYATGSITDGYLARGRLEAEGIPVLVKGEGEGPYRM
                     GPVYLWVPAGLEVQARMILDEVSEHRTSSEEDAGDPDDEAYAGWDDDA"
     gene            1017..2357
                     /gene="glmM"
                     /locus_tag="VE646_12200"
     CDS             1017..2357
                     /gene="glmM"
                     /locus_tag="VE646_12200"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HZD80784.1"
                     /translation="MGRLFGTDGVRGVFGRDLTTDLARDLGRAAVVVLRRDADGPLTF
                     VLGRDTRASGGPLEDALAEGIRSAGADVFLAGVQPTPAVAFLTTDLRASAGVVISASH
                     NPADDNGIKFFGPYGFKLSDALEDAIEEELRSGGNGSVRRGRVLPLPGGERSYLDHVA
                     LAAEAPLDGMRVVVDCANGAASEATPEALRRLGAEVHPIFDRPDGRNINDGCGAMHPE
                     VVARAVVELGADAGLAHDGDADRAMFADARGNLIDGDQVLAACATSLKADGLLEGDTV
                     VTTVMANLGLHQAMDRAGIRVISTNVGDRYVLEGMLQSGAVLGGEQSGHVIFRQHATT
                     GDGLLTAVRFLSLAARKGVTVAELASVMRRFPQVLENVRVERKDELGSSDAVREAVAA
                     VEADLDGRGRVLVRASGTEPIVRVMVEAESEQTAARHVAALAERVRSALGQPAG"
     gene            2380..4203
                     /gene="glmS"
                     /locus_tag="VE646_12205"
     CDS             2380..4203
                     /gene="glmS"
                     /locus_tag="VE646_12205"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016575940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZD80785.1"
                     /translation="MCGIVGYVGPDQALPIILEGLRCLEYRGYDSAGVALIDGELTVV
                     KRAGKIGELEAAVGGDGAPTGRVGIGHTRWATHGEPSDRNAHPHVDCGGRIAVIHNGI
                     VENFAALRAGLEKRGHRLRSDTDTECIAHLIEEQDGPLPDAVRSVVAQLEGAYAVVVV
                     SQNDPDVVVGAKVSSPLVVGLGRGENVLASDIPAVLSRTRTVVPLEEGQVVEVRADRV
                     RITDFLGHELDPVPMRVDWDASRAQKGGFDHFMLKEIHEQPAAIRDTLAGRVGAGGML
                     SLDELHIDPDVLREVGKVFVVACGTAFHSGLVAKYAIEHRTRLPVEIEIASEFRYRDP
                     VLGPDTLTLAVSQSGETIDTLEAARHAARQGSRVIAVTNTVGSSLTREADGVLYTHAG
                     PEIGVAATKTFATQMVALHLAALYLAQVRGSMFPAEVVEAVERMHELPQQVQRTLELN
                     DGVRDLARQIQDARDVLFIGRHVGYPAALEGALKLKEISYIHAEGYPAGELKHGPIAL
                     VEPGVPVVAVATECHVYPKVLSNIQEVRARGAEVIAVATAGDRHIGEVAQHVLFVPRT
                     PELLAPVVVTPPLQLLAYHIARLRGCDVDQPRNLAKSVTVE"
     gene            4257..4631
                     /locus_tag="VE646_12210"
     CDS             4257..4631
                     /locus_tag="VE646_12210"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HZD80786.1"
                     /translation="MEILGLGVDICEIARMERALARHPRMRERVFTPEERAYCEGRAR
                     PAESYAGRFAAREAVIKALGGYRGKGWQDIGVSRHPSGAPLIVLSGNAKRRADRLGVD
                     RVLITFTHERSHAVAFAVAVRG"
     gene            4628..6151
                     /locus_tag="VE646_12215"
     CDS             4628..6151
                     /locus_tag="VE646_12215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HZD80787.1"
                     /translation="MRPVLTPEQAGSLDRESQARGITADSLMERAGRHVARAAATVAG
                     GIYGRRAVVVCGKGNNGGDGLVAARALAGIGMRVAVILLGSPQELSGPAAANLARLDE
                     VGLRPRAFEPDHLARELGRADVAVDALFGTGFRGVPEGEWAQAIAALNEGGRPVVAVD
                     IPSGVNGATAAVEGEAVRADLTVTFGAAKVGVILPPGTGFAGLVEVADIGFPPDLVRT
                     DLWLMERADVAALLPERGFETHKRSAGVAVVIGGSRLMTGAVCLSAEAAYRAGAGLVT
                     VAVPEGILPVVQTRLRETTFIPLPETGEGTVAASIERLEQGLEAADAVAIGPGMTTQE
                     ETAAYVRTLVRSCPVPVLVDADGLNAFAGRADELADRRSPAVLTPHAGEFARLSGSSA
                     AEVTADRVGRARALAGRTGATVLLKGSRTVVAEPGGPVRVNPTGGPFLATGGTGDVLT
                     GIITGFLAQGMEPADAAACGAYVHGLAGLRAASSRGDGMVAGDLLEPVAEVLEELRT"
     gene            6151..7257
                     /gene="alr"
                     /locus_tag="VE646_12220"
     CDS             6151..7257
                     /gene="alr"
                     /locus_tag="VE646_12220"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZD80788.1"
                     /translation="MRRYRPTYVRVDLDAIRHNIGLLKPRGSELMAVLKADGYGHGDA
                     AVARGALDAGATWLGVALVEEGILLREAGVDARILVLSEFPPGSEKEALSHGLTPSVY
                     TEEGLAALAQAARAFGEPLGVHVKVDTGMHRVGRWPPRSVPGFARAVRAAGLGLEGLW
                     THFACAESDRAGTLSQLERLLEVDRAVRADGPGPAYRHAANSAATILYPEAHLEIVRT
                     GAAVYGLDPGGGIGPRFGLRPALSWRSAVTLARRLPAGERISYGWRYQLEHEATVATV
                     PVGYEDGYARALSSRAEVLIRGRRHPVAGTVTMDTILVDCGDAEVAVGDEVVLIGEQG
                     DQRITAEELGAHMGTIGYEVVTAISERVPREYRG"
     gene            7254..8315
                     /locus_tag="VE646_12225"
     CDS             7254..8315
                     /locus_tag="VE646_12225"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZD80789.1"
                     /translation="MTRRRLAVATAGLAAGAVAGGALSRVVLGRRRGDPEQDEILSRL
                     PPEDLGPVESFDGTRLAVRAAGDPQGPSLLFVHGFSLDMTAWHYQWTALSDRFRCVLF
                     DLRSHGRSGGAGPGGLSVEAMGRDLSAVLEATVRGPVILVGHSMGAMSIMAMAGLRSD
                     LFGDPVAGIAFVGSAAAELLRGAMGSVTELLRPRLGTLTQAAARMNRLRRYVLSAPTD
                     VAGLVARVTQFGPNASPHLVNYVVGLAARAPAAVWTEGLADLMEVDLRPSLRRIGVPS
                     LVMVGEHDRVTPPSSAVVMAGELPDAQLAVIEAAGHMAMLERHEEVNRRLSAFAHEAL
                     AAGRTGGVQEASNGGRGRA"
     gene            8312..9244
                     /locus_tag="VE646_12230"
     CDS             8312..9244
                     /locus_tag="VE646_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HZD80790.1"
                     /translation="MITAVSGIRVGHATDPAGLTGCTVVLCPPGTVGSGEVRGGAPAT
                     RETDLLRPGMLVNEVHAVLLTGGSAFGLSAAGGVMRYLEERGVGFPVGVAVVPIVPAA
                     ALFDLGVGDPGARPRPSEGYAACAAASEEVAEGSVGAGTGATVAKLHGPDRQVKGGIG
                     TACVEEDALVVGALFAVNAVGEILGEDGSVLAGARPGPEGEGDDAGGPAEPWPGGSWP
                     NTIIGVVATNARLSKERAHLLAIAAHEAIETVVRPAHTLWDGDTVFTLATGAVDAPQD
                     ALERTAATAVRGSIRRSVTTATGVAGAPASGEVV"
     gene            9241..9843
                     /locus_tag="VE646_12235"
     CDS             9241..9843
                     /locus_tag="VE646_12235"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008482017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZD80791.1"
                     /translation="MRTLEEAAAAAATCTRCRLSKGRTQVVYGVGSPSADLMFIGEAP
                     GFHEDRQGEPFVGAAGQLLNRMLAEAGIAREDVYINNVILCRPPGNRDPYPDEIDACS
                     PWLRERITLIDPRVIVTLGNWATRFILGKQVSISRVRGQRFTVEGRLVIPTFHPAAIL
                     HGGGDSSSQMTALRADFQEIHAALAEAPKPGGRQEQLGLF"
     gene            9843..10331
                     /gene="tsaE"
                     /locus_tag="VE646_12240"
     CDS             9843..10331
                     /gene="tsaE"
                     /locus_tag="VE646_12240"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HZD80792.1"
                     /translation="MRIELTSFTPEDTRTIGGALGQVLGPGDAVALTGELGAGKTTLV
                     QGVAAAVGFTGPVVSPTFTLVREYQGRPLRIFHVDVYRLERVQDVLDLGLDEMADQGG
                     VLLVEWGDAVEGLLPGDHLLVELWVPGPDEQRRISLAPTGASWQARWASLEAALGGWI
                     AG"
     gene            10316..11035
                     /gene="tsaB"
                     /locus_tag="VE646_12245"
     CDS             10316..11035
                     /gene="tsaB"
                     /locus_tag="VE646_12245"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HZD80793.1"
                     /translation="MDRRMIVLGIDTSTTQTSVALGTERGTLASLGFAGPARRDAVVP
                     ALDHLLSWSGLELSQVGGIAVGLGPGLFTGLRVGVVTAKTLAQVLTAPIVGIPSLDAL
                     AFRVRHTGRLIGAVIDARRGEVFYAFYRPVPGGVFREGNQQVGSPEHLAADLEALGED
                     VLLVGNGAILYGHGLETAGSGVELASPAEAYPPAPALVELAIPRFLREEQDRLTELVP
                     LYLRRSDAEIAWDQRRRGASA"
     gene            11046..11537
                     /gene="rimI"
                     /locus_tag="VE646_12250"
     CDS             11046..11537
                     /gene="rimI"
                     /locus_tag="VE646_12250"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HZD80794.1"
                     /translation="MKRRHLRGVMAIERQVYPRPWSPNLFLSEMSETRNRCYLVARID
                     REVVGYGGLIGYGEEAHVTNIAVDPLHHRRKLGSRLLHDLIQNAIAMGAEAVSLEVRV
                     TNWGAQRLYGRFGFRPVGVRRNYYQEIGEDALIMWLDDIRTPEYEQKLRALMEQLPEG
                     IRP"
     gene            11548..12549
                     /gene="tsaD"
                     /locus_tag="VE646_12255"
     CDS             11548..12549
                     /gene="tsaD"
                     /locus_tag="VE646_12255"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HZD80795.1"
                     /translation="MKVLGIETSCDETAVAVVEDGRIGSNLIGSQTDLHERFGGVVPE
                     VAARAHVEALNPLIGEALGRAGSGFADLDGVAVTVGPGLVGALLVGMAAAKAVALAAR
                     LPFVGVNHLEGHIWANFLEHGPPRPPYVCLVVSGGHTMLVWMPEPHRYEVLGQTVDDA
                     AGEAFDKIARFLGLGFPGGPALDRLARRGDPTAIRFPRAMMGSGDYDFSLSGLKTAVI
                     RHVKEERAAGRAVDPADLAASFQEAIVDVQVAKTVAAATNRGAGTVLLGGGVVANTRL
                     RERMQLAGTEAGLEVRFPSPALCTDNGAMIAAAGASRLERGERTPLDVAADPGLELA"
     gene            12546..13490
                     /locus_tag="VE646_12260"
     CDS             12546..13490
                     /locus_tag="VE646_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZD80796.1"
                     /translation="MTTVLVTGGAGFIGSHLCDRLLAEDHRVIAVDDLSTGRIANLAE
                     ARGYGKGFTFFNMDVRADGLASLFDRHRPEIVMHLAAQAGVRPSIADPVHDASVNVMG
                     LLNVLECAVRTGVRKVVYAASGGTLYGEPRRLPVKESSAAGSRPLSPYGISKKVALDY
                     LPFFERYRGVTYTALALGNVYGPRQDPHGEAGVISIFSSKMLSGEAPTIFGDGNQTRD
                     YVFIDDTVHAFALAAERGDGRLLNIGTGLETSVNGLYRMLAEIIGFPRGPERGPLPAG
                     ELRRCSLDNSAAAEALGWKPWTHLEDGLGETVAYLRGV"
     gene            complement(13472..13945)
                     /locus_tag="VE646_12265"
     CDS             complement(13472..13945)
                     /locus_tag="VE646_12265"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZD80797.1"
                     /translation="MELSNEIGRALRHARKSQGLTLREVAQRSGGRFKATSVAGYERG
                     ERKITVERFSQLAAFYQVDASRLLAYALRAWRGQPPQVIDLNRLEEVPGTEGEILTRF
                     ARDVLELRRQWSGERLSLRAGDLEVLATVSGHTSQDFLALIRAALGEPEPQTPRR"
     gene            14352..15839
                     /locus_tag="VE646_12270"
     CDS             14352..15839
                     /locus_tag="VE646_12270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZD80798.1"
                     /translation="MAAAARSLRSAMGGSCFVGVALPDAAGRLRLGWEEGEAPRAERR
                     RSAMRRRAFRTRTVRRIEVDAGRSLVLLPLLSDGTSLGVLEVGASTPELEAAAPAMQA
                     AAEEVATALRSLCAHAVRRRELETLERTTAFGHELLSASDPEESARTIVRFIFECSKR
                     PVAAWSITEGSDGYALASIHGIDVRRRRAMGASLGSIPAWDRLSEPDRRDLVERFVEL
                     AGIEHVVRLAGDGLLILADDQGPAGPSLRALWGLIEQILLLRASAAHAQRRSDELDLG
                     LAWIAHELRGPVLGVKAALEHLVDRGQAGDDPILLRRSLRELERLAGTTEGLLGWAVG
                     ARPLHLRQIDLGRLVDEAVESCILETGNDSILVLGSEGVVVRADPVQLRMGVANLIRN
                     ALSFSQAGEKVEVRVELANGSATIAVSDQGPGIAPAEGDIIFDPFMRGRASSRSRSGS
                     GLGLFIARRVVEAHGGRIWPESDGHGATFLIRLPAEEGVIGRCAS"
     gene            15827..16492
                     /locus_tag="VE646_12275"
     CDS             15827..16492
                     /locus_tag="VE646_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD80799.1"
                     /translation="MRVLIVDDHMLFAEAVRGALEAVGMQVLGVLGTGGQGVERALED
                     RPDLVLMDIALPDQSGLAAGMRILEIWPQACIVALTALEDRQLAEEALRVGFRGYLTK
                     DTPVTQFVNSIRAVMDGQQVIPHRLGPSAGQPVIPGEIGLLAAQLTEREREVLALLVQ
                     GTGGREIAQQLSISRNTVRTHVQSILTKLQVHSRLEAATFAVRHGLVDPGSGPARRGS
                     IAW"
     gene            16665..17390
                     /locus_tag="VE646_12280"
     CDS             16665..17390
                     /locus_tag="VE646_12280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD80800.1"
                     /translation="MTDGGGRSIRVLLADQHALFREALRTVLEREPDLEVVAEAGNGM
                     DALSQVERVLPHVAILDVDLPMSNGPRTASLVKERAPDCRVIVLSPQEDYRRLIEALD
                     AGASGYLTKEAPLEDLIHATRAVHRGETLVPPRMLGPLLTSLLRRKSELDHRHQRISR
                     LTPREREVLALLANGAENDTIARTLVISRQTARTHIQNILGKLEVHSRLEAVAFVRGS
                     GMLEDLVDLTQNGSGEQLSVIRS"
     gene            complement(17434..>19028)
                     /locus_tag="VE646_12285"
     CDS             complement(17434..>19028)
                     /locus_tag="VE646_12285"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HZD80801.1"
                     /translation="KPPLVAIGVTVNPSTISAQTETNVATSTKVAELAKSALGTSVPT
                     QELLKHVSADMTQGTQILRITFTASEPTAAARGADAFAQAYLEYRRQQAQDYVDAQTG
                     QANSQLEDLQATIAKLQGKIDHPPAGFDVPAAQSKLSFDKAQAQIISSKLANLQNLTI
                     DPGQVIDPANVPTAPISPRHPFDIALGILLGVGLGVGAALVRERNSDAVRNVNELEGI
                     LGVPVLASVPRTRWGTPERAALVVARGLRTPAADAYRRLRTNLLSILEPSNMKTILVT
                     SADRGEGKTTTLVNLGAALAEIGRDVVLVSADLRRPAFQRIFSANGQMGLSQVLTEGV
                     AASEVFQETIPHLRVVHAGSVPREQEPVNLLQREGMRRFLAECVEHADFVLIDSPPIL
                     GVPDTLVLAPSVDAILFVTDSWKTRWDDVVQAREQVKRAGGRLVAGSLNRVQVPRRSR
                     RAYGADHQLLSLHPRLFSGRRSLRATLDLGDGGGKKGEEPPRAVDAAATAAENGRTPT
                     GNGSAPAEDGSPALQQGDPAAG"
BASE COUNT         2534 a         6443 c         7360 g         2691 t
ORIGIN      
        1 gaccgctccc gcctcggcga ccacgaccca gggcgaggcc ccggccgagg ctgaggccga
       61 ggcgccggcg gcgaagcgct ccacggggaa gcgctccacc gccaagaaga ccaccgccaa
      121 gaagacggcg gccaagaaga cggcggccaa gaagaccacc gccaagaaga cggcggccaa
      181 gacgtcggcg gccaagcggt ccgctgcgaa gaagagcacc gcccgccggg cgaagaagga
      241 ggagtagccc gtggcccagg ccgtgaacac ctcgacgggc cgccgcaagg aggccatctg
      301 ccgggtccgc ctggtggcgg gcagcggttc ctaccacctg aacggccgcc cgctcgagga
      361 ttacttcccc acgcgcgccc accgcatgat cgtgcagagc ccgctgaagc tggtgggacg
      421 ggacaaggac tacgacgtcc tcgcccggat cgagggcggc ggcgtggccg gccaggccgg
      481 cgcgctgcgg ctgggtatcg cgcgcgcgct catcgatctc gacccggagt tccggggcgt
      541 cctgaagaag gagggcatgc tctcccgcga cgcccgcgaa aaggagcgcc gcaagtacgg
      601 cctgaagaag gcgcgcaagg ccccccagta ctcgaagcgc tgaccggcgg cggcctcatc
      661 ggaggcctcc ccaccggcga gggctacgcg tcgtcgtccc agcccgcgta ggcctcgtcg
      721 tccgggtcgc ccgcgtcctc ctcggacgag gtgcggtgtt cgctcacctc atccaggatc
      781 atccgggcct ggacctcgag ccccgccggg acccacaggt acacggggcc catccggtag
      841 gggccctctc cctcgccctt caccagcacg gggatgccct cggcctccag ccgtccccgg
      901 gccaggtagc cgtcggtgat cgacccggtc gcgtacacac gaacgaagtc ctcatcgccc
      961 acccccccat cgtcccactc gaacgcccgg tccgttcggg tagccttggg tcgcggatgg
     1021 gacggctctt cggcaccgac ggcgtgcgcg gcgtgttcgg ccgcgatctg accaccgacc
     1081 tggctcggga tctggggcgg gccgccgtgg tcgtccttcg tcgagatgcc gacggaccgc
     1141 tgacgttcgt gctcgggcgc gacaccaggg cttcgggagg gcccttggag gacgcgttgg
     1201 ccgaggggat ccggtcggcc ggcgccgacg tgtttctggc cggcgtccag cccacgccgg
     1261 cggtggcctt tctgaccacg gacctcaggg cctcggccgg ggtggtgatc tcggcatcgc
     1321 acaacccggc cgacgacaac ggcatcaagt tcttcgggcc ctacgggttc aagctctccg
     1381 acgccctgga ggacgcgatc gaggaggagc tccgttcggg cggcaacgga tcggtgcggc
     1441 ggggccgggt cctgccgctg ccggggggcg agcgcagcta cctggaccac gtggccctgg
     1501 cggccgaagc gcccctggac gggatgcgcg tggtggtgga ctgcgcgaac ggcgcggcct
     1561 ccgaggccac ccccgaggcc ctgcgccgcc tgggggccga ggtccacccc atcttcgacc
     1621 ggcccgacgg ccgcaacatc aacgatgggt gcggcgccat gcatccggag gtggtggccc
     1681 gggcggtggt ggagctcggg gccgacgccg ggctggccca cgacggcgac gccgaccggg
     1741 cgatgttcgc cgacgcccga gggaacctga tcgacgggga ccaggtgttg gcggcctgcg
     1801 ccaccagcct caaggcggac ggcctgctcg agggcgacac ggtggtcacc accgtgatgg
     1861 cgaacctcgg gctccaccag gccatggacc gggccgggat ccgggtgatc tccaccaacg
     1921 tgggggaccg ctacgtgttg gagggcatgc tgcagagcgg tgcggtgctg ggcggggagc
     1981 agtccggcca cgtgatcttc cgccagcacg ccaccaccgg ggacggtctg ctcacggcgg
     2041 tgcggttcct gtcgctggcc gctcgcaagg gggtcacggt ggccgaactg gcctcggtga
     2101 tgcgacggtt cccccaggtg ctggagaacg tgcgggtgga gcgcaaggac gagctcgggt
     2161 cctccgacgc cgttcgggag gccgtcgcgg ccgtggaggc ggacctggac gggaggggca
     2221 gggtgctggt gcgggcctcg ggaaccgagc ccatcgtccg ggtcatggtc gaggccgaga
     2281 gcgagcagac ggccgcccgg cacgtggcgg cgctggccga gcgggtccgt tccgcccttg
     2341 gccagccggc cgggtaggcc ctgttaggct gaggccccca tgtgcggcat cgtcggctac
     2401 gtggggcccg atcaggccct gcccatcatc ctggaggggc tccggtgcct ggagtaccgc
     2461 ggctacgact cggcgggcgt ggccctgatc gacggcgagc tgaccgtcgt caagcgcgcc
     2521 gggaagatcg gggagctgga ggccgcggtg ggcggggacg gcgcgcccac cggacgggtg
     2581 ggcatcggtc acacgcggtg ggccacccac ggggagccct cggatcgaaa cgcgcatccc
     2641 catgtggact gcggcggtcg gatcgccgtg atccacaacg gcatcgtgga gaacttcgcg
     2701 gcgctccggg cggggctgga gaagcgcggt caccgcctgc gctcggacac ggacaccgag
     2761 tgcatcgccc acctcatcga ggagcaggac ggccccctgc ccgacgccgt gcgctcggtg
     2821 gtggcccagc tggagggcgc ctacgcggtg gtggtggtgt cccagaacga cccagacgtg
     2881 gtggtggggg cgaaggtctc ctccccgctg gtggtgggcc tgggtcgggg cgagaacgtc
     2941 ctggcctcgg acatcccggc cgtcctgtcg cgcacccgca ccgtggtccc gctggaggag
     3001 ggccaggtgg tggaggtgcg ggccgaccgc gtccggatca ccgacttcct gggtcacgag
     3061 ctcgacccgg tccccatgcg ggtggactgg gacgcctctc gggcacagaa gggtgggttc
     3121 gaccacttca tgctgaagga gatccacgag cagccggcgg cgatccgcga cacgctcgcc
     3181 ggccgggtcg gggccggggg gatgctctcg ctggacgagc tccacatcga tcccgacgtg
     3241 ctgcgcgagg tcggcaaggt gttcgtggtg gcgtgcggga ccgccttcca ctcggggctg
     3301 gtggccaagt acgcgatcga gcaccggacc cggctgccgg tggagatcga gatcgcctcc
     3361 gagttccgct accgcgatcc cgtcctcgga ccggacacgc tgaccctggc ggtcagccag
     3421 tcgggggaga cgatcgacac gctggaggcg gcccgccacg ccgcccgcca gggatcgcgg
     3481 gtgatcgcgg tgacgaacac ggtggggtcc tcgctgaccc gggaggccga cggcgtgctg
     3541 tacacccacg ccgggcccga gatcggggtg gccgccacca agaccttcgc cacccagatg
     3601 gtggccctgc acctcgccgc cctgtacctg gcccaggtgc ggggctcgat gttcccggcc
     3661 gaggtcgtgg aggcggtcga gcgcatgcac gagctccccc agcaggtgca gcggaccctg
     3721 gagctgaacg atggggtgcg ggacctggcc cggcagatcc aggacgctcg cgacgtcctg
     3781 ttcatcggcc ggcacgtggg gtacccggcc gcgctcgagg gggccctgaa gctgaaggag
     3841 atctcctaca tccacgccga gggctacccg gccggcgagc tgaagcacgg gcccatcgcc
     3901 ctggtcgagc ccggcgtccc ggtggtggcc gtggccaccg agtgccacgt ctatcccaag
     3961 gtgctgtcga acatccagga ggtccgggct cggggcgccg aggtgatcgc cgtggccacc
     4021 gcgggggacc ggcacatcgg ggaggtggcg cagcacgtgc tgttcgtgcc ccgcaccccc
     4081 gagctcctgg ccccggtggt ggtgaccccg cccctccagc tgctcgccta ccacatcgcg
     4141 aggctccgag ggtgcgacgt ggaccagccc aggaacctgg ccaagtcggt gacggtcgag
     4201 tagccggccg cgggaaggcc cgttcccgcc gttcccaccc ggctaaccta tgcgccgtgg
     4261 agatcctcgg cctgggcgtg gacatctgcg agatcgcccg gatggagcga gcgctggccc
     4321 gccacccccg gatgcgcgag cgcgtcttca cgcccgagga gcgcgcctac tgtgaggggc
     4381 gggctcgccc cgccgagtcc tacgcgggcc gcttcgccgc gcgcgaggcc gtgatcaagg
     4441 ccctcggcgg ctaccggggg aagggctggc aggacatcgg cgtgagccgg catccgagcg
     4501 gcgctccgct catcgtgctg tcgggcaacg ccaagcgacg ggccgatcgg ctgggggtcg
     4561 accgggtgct gatcaccttc acccacgagc ggtcccacgc cgtggccttc gcggtggcgg
     4621 tgcgcggatg aggccggtcc tgacccccga gcaggccggg agcctggacc gggagagcca
     4681 ggcccgaggg atcacggccg actcgctgat ggagcgggcc gggcggcacg tggcccgggc
     4741 cgcggccacg gtggccggcg ggatctacgg acgccgggcg gtggtggtgt gcggcaaggg
     4801 caacaacggg ggcgatgggc tggtggcggc tcgggccctg gccgggatcg ggatgcgggt
     4861 ggccgtgatc ctgctcggct cgccccagga gctcagcggt ccggcggccg ccaacctggc
     4921 ccggctggac gaggtcggcc tgcgcccccg ggcgttcgag ccggaccacc tggcccgcga
     4981 gctgggacgg gccgacgtgg cggtcgatgc gctgttcggc accgggttcc gcggtgtccc
     5041 cgagggcgag tgggcccagg cgatcgccgc gctgaacgag gggggcaggc ccgtggtggc
     5101 cgtcgacatc ccctcgggcg tgaacggcgc gaccgccgcg gtggagggcg aggccgtgcg
     5161 ggcggacctg acggtcacct tcggggccgc caaggtgggc gtgatcctgc caccggggac
     5221 cgggttcgcc ggcctggtgg aggtggccga catcggcttc ccgccggacc tggtccgcac
     5281 cgacctgtgg ctgatggagc gggccgacgt ggcggccctc ctgcccgagc gggggttcga
     5341 gacgcacaag cgctcggccg gcgtggcggt ggtgatcggg ggatcgcggc tgatgaccgg
     5401 ggcggtgtgc ctgtcggccg aggccgcgta ccgggcggga gccgggttgg tcacggtggc
     5461 ggtgcccgag gggatcctgc ccgtggtcca gacccggctg cgcgagacga ccttcatccc
     5521 cctcccggag accggggagg gcacggtggc cgcgtcgatc gagcggctgg agcagggcct
     5581 ggaggccgcc gatgcggtgg ccatcgggcc cggcatgacc acgcaggagg agaccgccgc
     5641 ctacgtgcgg accctggtcc gctcgtgccc cgtgccggtg ctggtcgacg cggatggcct
     5701 gaacgccttc gccggccggg ccgacgagct ggccgaccgg cgctccccgg ccgtgctgac
     5761 cccgcacgcg ggcgagttcg cgcgcctgtc cggctcgagc gcggccgagg tcaccgccga
     5821 ccgggtgggc cgggcccggg cgctcgccgg gcgcaccggg gccacggtgc tgctcaaggg
     5881 gagccgcacc gtggtggccg agcccggtgg gccggtccgg gtcaacccca ccgggggccc
     5941 gttcctggcc accgggggga cgggggacgt gctgaccggt atcatcaccg gtttcctggc
     6001 ccagggcatg gagccggcgg atgcggccgc ctgcggcgcc tacgtgcacg ggctggccgg
     6061 tcttcgtgcc gcctccagcc ggggcgacgg gatggtggcg ggcgacctgc tcgagcccgt
     6121 ggccgaggtc ctcgaggagc tgaggacgtg atgcgccggt accggccgac gtacgtccgg
     6181 gtggacctgg acgcgatccg acacaacatc ggtctgctga agccccgggg ctcggagctc
     6241 atggccgtgc tgaaggcgga cgggtacggt cacggcgacg cggcggtggc caggggagcg
     6301 ctggacgccg gtgccacctg gctcggcgtg gcgctggtgg aggaggggat cctcctacgc
     6361 gaggcgggcg tggatgctcg gatcctggtc ctttccgagt tccccccggg ctcggagaag
     6421 gaggccctat cccacgggct caccccctcg gtctacacgg aggaggggtt ggccgcgctg
     6481 gcccaggcgg cccgcgcctt cggcgagccc ctcggggtgc acgtgaaggt ggacaccggc
     6541 atgcaccggg tgggtcggtg gccgccgcgg tccgtgcccg ggttcgcccg ggcggtgcgg
     6601 gcggccgggc tcggcctgga gggcctgtgg acccacttcg cgtgcgccga gtccgatcgc
     6661 gccgggacgc tgagccagct ggagcgactg ctcgaggtgg accgggcggt ccgggccgac
     6721 ggtccggggc ccgcgtaccg gcacgccgcg aacagcgcgg ccaccatcct gtacccggag
     6781 gcgcacctgg agatcgtgcg gaccggggcg gccgtgtacg ggctggatcc ggggggcggg
     6841 atcggtccgc ggttcggcct gcgtcccgcg ctcagctggc gctcggcggt cacgctggcc
     6901 cggcggctgc cggccgggga gcggatctcg tacggttggc ggtaccagct cgagcacgaa
     6961 gccaccgtgg ccaccgtgcc ggtgggctac gaggacgggt acgcccgtgc gctgtcctcg
     7021 cgcgccgagg tcctgatcag ggggcggcga caccccgtgg ccgggaccgt cacgatggac
     7081 acgatcctgg tggactgcgg ggacgccgag gtggcggtgg gggacgaggt ggtgctgatc
     7141 ggcgagcagg gggatcagcg gatcacggcg gaggagctcg gggcgcacat ggggacgatc
     7201 gggtacgagg tggtcacggc catctccgaa cgggtgccgc gggagtatcg gggatgacca
     7261 ggcgccgctt ggccgtggcc accgccgggt tggccgccgg ggccgtggcc ggcggcgcgc
     7321 tgagccgggt ggtcctgggc cggcgacggg gcgaccccga gcaggacgag atcctctccc
     7381 ggctcccgcc ggaggacctg gggccggtgg agtcgttcga cgggacgcgc ctcgcggtgc
     7441 gggcggccgg cgacccgcag gggccgagcc tgctgttcgt gcacggcttc agcctggaca
     7501 tgaccgcctg gcactaccag tggacggcgc tgtccgatcg cttccggtgc gtgctcttcg
     7561 accttcgctc gcacgggcgc agcggtggtg ccggtccggg agggctgtcc gtcgaggcca
     7621 tgggccggga cctgtcggcg gtgctggagg ccacggtccg ggggcccgtg atcctggtgg
     7681 gccacagcat gggcgcgatg tcgatcatgg ccatggccgg gttgcgctcg gacctgttcg
     7741 gcgatccggt ggccgggatc gcgttcgtcg gctcggccgc ggcggagctg ctgcggggcg
     7801 ccatgggctc ggtgaccgag ctgctccggc cgcgcctggg caccttgacc caggcggccg
     7861 cgaggatgaa ccgcctgcgc cggtacgtgc tctcggcgcc cacggacgtg gccgggctcg
     7921 tggcccgggt cacccagttc ggaccgaacg cctcccccca cctggtgaac tacgtggtgg
     7981 ggctggccgc tcgggccccg gccgcggtat ggaccgaggg gttggccgac ttgatggagg
     8041 tggatctgcg gccctccctc cggcggatcg gggtgccgtc gctggtgatg gtgggggagc
     8101 acgaccgggt gacgccgccg tcctcggccg tggtcatggc cggcgagctc cccgacgcac
     8161 agctggcggt gatcgaggcg gccgggcaca tggccatgct cgagcgccac gaggaggtca
     8221 accggcggct gtcggccttc gcccacgagg cgctggccgc cgggcggacc gggggggtcc
     8281 aagaggcgtc gaacggcggg agggggcggg cgtgatcacg gcggtgagcg gcatccgggt
     8341 gggtcatgcc accgaccccg cgggcctcac gggctgcacg gtcgtgctgt gcccaccggg
     8401 cacggtcggc agcggcgagg tccggggcgg cgcgccggcc acgcgggaga cggacctgct
     8461 gcgacccggc atgctggtga acgaggtcca cgcggtactc ctcaccgggg gcagcgcctt
     8521 cggcctgtcc gccgccgggg gcgtgatgcg gtacctggag gagcgggggg tggggttccc
     8581 ggtcggggtg gcggtggtgc cgatcgtccc cgcggccgcg ctgttcgacc tgggggtcgg
     8641 ggacccgggc gcccgtcccc ggcccagcga gggctacgcg gcctgcgcag cggcctcgga
     8701 ggaggtggcc gagggcagcg tgggggccgg gaccggggcc acggtggcca agctccacgg
     8761 cccggaccga caggtgaagg gcgggatcgg taccgcctgt gtggaggagg acgcgctggt
     8821 cgtgggcgcg ctgttcgcgg tgaacgcggt gggcgagatc ctcggggagg acggatccgt
     8881 gctggccggg gcccgaccgg gccccgaggg cgagggtgat gacgcgggtg ggcccgccga
     8941 gccctggccg ggaggttcct ggccgaacac gatcatcggg gtcgtggcca ccaacgccag
     9001 gctctcgaag gagcgcgcgc acctgctggc gatcgccgcg cacgaggcga tcgagacggt
     9061 ggtgcgaccc gcccacaccc tgtgggacgg ggatacggtg ttcacgctcg ccaccggtgc
     9121 cgtggatgcg ccgcaggacg cgctggaacg gacggcggcc acggcggtga ggggatcgat
     9181 ccggcggagc gtgacgacgg cgacgggggt ggccggggcg ccggcgtcgg gggaggtcgt
     9241 atgaggacgc tggaggaagc ggcggcggcg gcggcgacgt gcaccagatg ccgtctgtcg
     9301 aaggggcgga cgcaggtcgt gtacggcgtg ggcagcccaa gcgccgacct gatgttcatc
     9361 ggggaggccc cagggttcca cgaggaccgg cagggcgagc ccttcgtcgg cgcggccggg
     9421 cagctgctga accgcatgct cgccgaggcc ggcatcgccc gcgaggacgt gtacatcaac
     9481 aacgtgatcc tgtgccggcc ccccggcaac cgcgatccct acccggacga gatcgacgcg
     9541 tgttccccgt ggctccgaga gcgcatcacc ctgatcgatc ctcgggtgat cgtgacgctc
     9601 gggaactggg cgacccgctt catcctgggc aagcaggtct cgatcagccg ggtccgaggt
     9661 cagcggttca cggtggaggg ccggctggtg atccccacct tccatcccgc cgcgatcctg
     9721 cacggcggcg gggacagctc ctcgcagatg accgccctcc gggcagactt ccaggagatc
     9781 cacgccgcgc tggccgaggc cccgaagccg ggggggcgac aggagcagct cgggctgttc
     9841 tgatgcggat cgagctcacc tccttcacgc ccgaggacac ccggacgatc ggtggggcgc
     9901 tcggtcaggt gctggggccc ggggacgccg tggcgctcac cggcgagctg ggtgccggca
     9961 agaccacgct ggtccagggg gtcgccgcgg cggtcgggtt caccgggccg gtcgtgtcgc
    10021 ccaccttcac gctggtgcgc gagtaccagg gccgcccact ccggatcttc cacgtggacg
    10081 tctaccggct ggagcgcgtg caggacgtgc tcgacctggg gctggacgag atggccgatc
    10141 agggaggcgt gctgctggtg gagtggggcg atgcggtgga ggggctgctg cccggcgatc
    10201 acctgctcgt ggagctctgg gtgcccggtc cggatgagca gcgtcggatc tcgctggctc
    10261 cgaccggcgc ctcctggcag gcccggtggg cgagcctgga ggcggccctg gggggatgga
    10321 tcgccggatg atcgtcctgg ggatcgacac gtccaccacg cagacctcgg tggcgctggg
    10381 caccgagcgg gggacgctgg cgagcctggg gttcgccgga ccggcgcgcc gggacgccgt
    10441 ggtcccggcc ctggatcatc tgctgtcctg gagcggcctg gagctgtcgc aggtcggggg
    10501 catcgcggtg ggcctggggc cggggctgtt caccgggttg cgggtgggcg tcgtgaccgc
    10561 caagacgctg gcgcaggtcc tgaccgcccc catcgtgggg atcccgtcgc tggacgcgtt
    10621 ggccttccgc gtgcggcaca cgggccggct gatcggggcg gtgatcgatg cccggcgggg
    10681 cgaggtgttc tacgccttct accgtccggt tcccggcggg gtgttccggg aagggaacca
    10741 acaggtcggc tcgccggagc acctggcggc ggacctggag gcgctggggg aagacgtgct
    10801 gctcgttggg aacggtgcca tcctgtacgg tcatgggctg gagacggcgg ggagtggcgt
    10861 cgagctcgcc tcgcccgccg aggcctaccc cccagccccg gcgctggtgg agctggccat
    10921 cccgcgcttc ctccgggagg agcaggaccg cctgaccgag ctcgtgcccc tgtacctcag
    10981 gagatcggat gcggagatcg cttgggacca gcgacgtcga ggcgcctcgg cttgaggtga
    11041 cccgcatgaa gcgccgccat ctccgcggcg tgatggcgat cgagcgccag gtctatccgc
    11101 gcccctggag cccgaacctg ttcctgtcgg agatgagcga gacccgcaac cgctgctacc
    11161 tggtggcccg gatcgatcgc gaggtggtgg gctacggcgg cctgatcggg tacggggagg
    11221 aggcccacgt cacgaacatc gcggtggacc ccctccacca ccgccgcaag ctcggctcgc
    11281 gcctgctgca cgacctgatc caaaacgcga tcgccatggg cgccgaggcc gtctcgctcg
    11341 aggtgcgggt cacgaactgg ggcgcccagc gcctctacgg gcggttcggc ttccggccgg
    11401 tcggggtgcg ccgcaattac tatcaggaga tcggcgagga cgccctgatc atgtggctgg
    11461 acgacatccg cactcccgag tacgagcaga agctgcgcgc cctgatggag cagctgcccg
    11521 aaggcatccg cccgtgagcg aggccgggtg aaggtcctcg gcatcgagac gtcctgcgac
    11581 gagacggcgg tcgcggtggt ggaggacggt cggatcggct ccaacctgat cgggagccag
    11641 accgacctgc acgagcgctt cggcggcgtg gtccccgagg tcgcggcccg ggcccacgtg
    11701 gaggccctga acccgttgat cggggaggcc ctgggccgcg ccggatccgg gttcgccgat
    11761 ctggatggcg tggcggtcac ggtcggaccc gggctggtgg gcgcgctgct ggtcggcatg
    11821 gccgcggcca aggcggtggc cctggcggcg cgcctgccct tcgtcggggt gaaccacctg
    11881 gaggggcaca tctgggcgaa cttcctcgag cacggtccgc cccggccccc gtacgtctgc
    11941 ctggtggtct ccggcggcca cacgatgctg gtctggatgc cggagccgca ccggtacgag
    12001 gtgctggggc agacggtgga cgatgccgcc ggcgaggcgt tcgacaagat cgcccggttc
    12061 ctcggtctgg gcttccccgg gggcccggcc ctggatcgcc tcgctcggcg aggcgaccca
    12121 accgcgatcc ggttcccccg ggccatgatg ggctcgggcg actacgactt ctcgctgtcg
    12181 gggctgaaga cggcggtgat ccgccacgtg aaggaggagc gggccgcggg acgcgcggtg
    12241 gacccggccg acctggccgc ctccttccag gaggcgatcg tcgacgttca ggtggccaag
    12301 accgtcgccg ccgccacgaa ccggggggca ggcacggtgc tgctgggggg cggcgtggtg
    12361 gcgaacaccc gtctgcgcga gcggatgcag ctggcgggga cggaagcggg gctggaggtg
    12421 cggttcccct cgccggcgct gtgcaccgac aacggcgcga tgatcgcggc ggcgggcgcc
    12481 tcccggctgg agcggggaga gcgcaccccg ctcgacgtgg cggccgaccc ggggctggag
    12541 ctggcgtgac cacggtcctg gtgacgggcg gagcggggtt catcggctcg cacctgtgcg
    12601 accgcctgct ggccgaggac caccgggtga tcgccgtgga cgacctgtcc accgggcgga
    12661 tcgccaacct cgccgaggcg cgcggctacg gcaagggctt cacgttcttc aacatggacg
    12721 tccgggccga tggtctggcc tccctgttcg atcggcatcg ccccgagatc gtgatgcatc
    12781 tggccgcgca ggccggggtg cgcccctcga tcgcggaccc ggtccacgac gccagcgtga
    12841 acgtgatggg cctgctgaac gtcctggagt gcgcggtccg cacgggggta cgcaaggtcg
    12901 tctacgccgc ctctggaggg acgctgtacg gcgaaccccg acgcctgccc gtcaaggagt
    12961 cgtccgccgc cgggtcgcgc ccgctgtccc cgtacggcat cagcaagaag gtggccctgg
    13021 actacctgcc gttcttcgag cgctaccggg gagtgaccta cacggccctc gcgctcggca
    13081 acgtctacgg gcccaggcag gatccgcacg gcgaggccgg ggtgatcagc atcttctcct
    13141 cgaagatgct ctcgggcgag gcccccacga tcttcggcga cggcaaccag acccgcgact
    13201 acgtgttcat cgacgacacc gtccacgcct tcgccctggc cgctgagcgg ggcgacggcc
    13261 ggctcctgaa catcgggacc ggcctggaga cctcggtgaa cgggctgtac cggatgctgg
    13321 cggagatcat cgggttcccc aggggaccgg agcgggggcc gctccccgcc ggcgagctcc
    13381 gccgctgctc gctggacaac tcggcggcgg ccgaagccct gggctggaag ccgtggacgc
    13441 acctggagga cggtctgggg gagacggtcg cctacctgcg aggcgtctga ggttccggtt
    13501 cgcccagcgc cgcccggatc agggcgagga agtcctggct ggtgtggccg ctcacggtgg
    13561 ccaggacctc gaggtcaccg gcccgcagcg acaggcgctc cccgctccat tgtcgccgca
    13621 gctccagcac gtcccgcgcg aaccgggtca ggatctcgcc ctcggtcccc ggcacctcct
    13681 ccaggcggtt caggtcgatc acctgcgggg gttgccctcg ccaggcacgc agggcgtagg
    13741 ccagcaggcg cgaggcgtcc acctggtaga aggccgccag ctgggagaac cgttcgaccg
    13801 tgatcttgcg ctccccacgc tcgtacccgg ccacggaggt ggccttgaac cgccctccgg
    13861 agcgctgagc cacctctcgc agggtcagac cctgcgactt gcgtgcatgc ctgagcgccc
    13921 gaccgatctc gttcgacagc tccatcaccg ctccctggcc gcggacacgt ttcgacctcg
    13981 ctcatcccgg ggaagcgggc tcagtgtatg gcgtccaggt cgtcagttcc actcgccgaa
    14041 tcaggaaatc tggcgtacgt tgtctacgca cgggccgaga ggggtaggtt ccatcttccc
    14101 gcatgtcggg cctctggctg ccgcatgcct ggaacgctct gggtgcaggg aaagggtgac
    14161 aagaacacga tctgcataca tttcctcttg tcaggacgcg atacgcgtcc tacactgccc
    14221 tacgtcgttg acagtaggga caacgatcca atccctggac atcctacgcc gatcggggga
    14281 gagagcccgt gacccacgtt cctgaccagg cgctgtacgg tacctggcgt acgctcggcg
    14341 gcttggctga tgtggcggcc gccgcgcggt ccctccgctc cgcgatgggc ggaagctgct
    14401 tcgtcggggt ggccctgccc gatgccgcgg ggcgcctgcg gctggggtgg gaggagggag
    14461 aggcgccacg ggccgagcga cgccgatcgg ccatgcgtcg ccgggccttc cggacccgaa
    14521 ccgtgcgacg gatcgaggtg gatgcgggcc gctcgctggt gctgctgccc ctcctcagcg
    14581 atggaacctc gctcggggtg ctggaggttg gtgcctccac cccggagctc gaagcggccg
    14641 cgccggccat gcaggccgcc gcggaggagg tcgcgacggc gctccggagc ctgtgcgcgc
    14701 acgccgtccg acggcgcgag ctggagaccc tggagcgcac gaccgccttc ggccacgagc
    14761 tcctatccgc atcggatccg gaggagtcgg cccgtaccat cgtgcgcttc atcttcgagt
    14821 gctcgaagcg gcccgtcgcc gcatggtcga tcaccgaggg cagcgacggc tacgcgctcg
    14881 cgtccatcca cgggatcgac gttcgccggc gccgggccat gggcgcctcg ctggggtcga
    14941 tcccggcgtg ggatcgactg tccgagccgg atcggcggga cctggtggag cgcttcgtgg
    15001 agctggccgg catcgagcac gtcgtgcggc tggccgggga cggcctcttg atcctggcgg
    15061 acgaccaggg gccggccggc ccgtcgctcc gcgcgctgtg gggcctcatc gagcagatcc
    15121 tgctgctgcg tgccagcgcg gcccacgcgc aacggcgcag cgacgagctg gacctggggc
    15181 tcgcctggat cgcccacgag ctccgtgggc ccgtgctcgg cgtgaaggcg gcgctggagc
    15241 acctggtgga ccggggacag gccggggacg atccgatcct cctgcgccgg tccctgcgcg
    15301 agctggagcg cctggccggc acgaccgagg ggctgctggg atgggccgtg ggggcacgcc
    15361 cgctgcatct tcgccagatc gatctggggc ggttggtgga cgaggcggtc gagtcctgca
    15421 tcctggagac cgggaacgac tccatcctgg tgctcggctc cgagggggtc gtggtccggg
    15481 ccgatcccgt gcagctgcgg atgggcgtcg cgaacctgat ccgcaacgcc ctgagcttct
    15541 cccaggccgg agagaaggtg gaggttcggg tggagctcgc caacggatcg gccaccatcg
    15601 cggtctcgga tcaaggtccg gggatcgctc cggcggaggg ggatatcatc ttcgatccat
    15661 tcatgagagg cagggcctcc tcccgatcgc gttccgggag cggcctgggt ctgttcatcg
    15721 cccggcgggt ggtggaggcc cacgggggga ggatctggcc tgagtcggac ggtcacggtg
    15781 ccacgttcct catccggctc cctgccgagg aaggagtgat cggccgatgc gcgtcttgat
    15841 cgtggacgac cacatgctct tcgccgaggc ggtgcggggc gccctggagg ccgtgggtat
    15901 gcaggtgctc ggcgtgctgg gcacgggtgg gcagggggtg gagcgggcgc tcgaggaccg
    15961 gccggacctg gtactcatgg acatcgccct gccggaccag agcgggctgg cggccgggat
    16021 gcggatcctc gagatctggc cccaggcctg catcgtcgcg ctgaccgcgc tcgaggaccg
    16081 tcagttggcg gaggaggccc tgcgggtggg cttccgcggc tacctgacga aggacacccc
    16141 ggtcacgcag ttcgtgaact ccatcagggc cgtcatggac ggtcagcagg tgatcccgca
    16201 ccggctggga ccctcagccg gtcagcccgt gatcccgggc gagatcggtc tgctggccgc
    16261 gcagctcacc gagcgcgagc gcgaggtgct cgcgctgctg gtccagggca ccggcgggcg
    16321 cgagatcgcg cagcagctga gcatcagcag gaacacggtg cggacgcacg tgcagagcat
    16381 cctcacgaag ctgcaggtgc attcgcgact ggaggcggcg accttcgccg tccgccacgg
    16441 cctggtggat ccggggtcag ggccggcgcg gcgagggagc atcgcgtggt gagcgttccc
    16501 gacccgagac cgcacctcga ggttcgctcc gtggttcggg gcgcgggtgg tcgcccggtt
    16561 catgcggatg tcgtagacgg cgtacaccat cgggggcgtg cacggctcac ccgatgccgg
    16621 taccgtccgc aggatcgggg aatgagcgag cgaagggcag gccgatgacc gatgggggag
    16681 ggcgctcgat ccgggtactg ctggccgacc agcacgccct gttccgagag gccctacgga
    16741 ccgtccttga gcgcgagccc gacctggagg tcgtggccga ggccggcaac ggcatggacg
    16801 cgctgagcca ggtggagcgg gtgctcccgc acgtggccat cctggacgtg gacctgccca
    16861 tgagcaacgg ccctcggacg gcctcgctgg tgaaggagcg ggcgcccgat tgccgggtga
    16921 tcgtgctgag cccgcaggag gactaccgcc ggctgatcga ggcgctcgat gccggcgcca
    16981 gcggctacct gaccaaggag gcgccgctcg aggacctgat ccacgccacc cgggccgtgc
    17041 accgagggga gaccctggtg ccgccccgga tgctggggcc gctgctcacc tcgctgctgc
    17101 gacggaagag cgagctcgac catcgtcacc agcggatctc gcgcctcacg ccgcgggagc
    17161 gcgaggtcct ggcgctgctg gccaacggcg cggagaacga cacgatcgcg cggaccctcg
    17221 tgatcagccg gcagaccgcc cgcacgcaca tccagaacat cctgggcaag ctggaggtgc
    17281 actcccggct ggaggcggtg gccttcgtgc ggggcagcgg catgctcgag gacctggtcg
    17341 acctcacgca gaacgggtcc ggggagcaac tgagcgtcat ccgttcctaa ccgaaccggt
    17401 tcgtcccgac gggggacgac ccttcggtcc tcctcagccc gcggccgggt ctccctgctg
    17461 cagggccggg gagccatcct cggcgggcgc ggagccgttg ccggtcggcg tgcgcccgtt
    17521 ctccgccgcg gtcgctgccg cgtcgaccgc acggggcggc tcctccccct tcttcccgcc
    17581 cccgtcgccc agatcgagcg tggcccggag gctccggcgg ccggagaaga gcctggggtg
    17641 caggctgagc agttggtggt cggccccgta ggccctgcgg ctgcgcctgg ggacctgcac
    17701 gcggttcagg gaccccgcca ccaaccggcc gccggcccgc ttgacctgct cgcgggcttg
    17761 gaccacgtcg tcccagcggg tcttccagga gtccgtcacg aacaggatcg cgtccacgga
    17821 cggcgccagc accagggtgt ccggcacgcc caggatcggc gggctgtcga tcaggacgaa
    17881 gtcggcgtgc tcgacgcact cggccaggaa gcggcgcatg ccctcccgct ggaggaggtt
    17941 gaccggctcc tgctcccggg ggacggaccc ggcgtggacc acccgcaggt gcgggatcgt
    18001 ctcctggaag acctccgagg cggccacgcc ctcggtcagc acctggctga ggcccatctg
    18061 gccgttggcc gagaagatcc gctggaaggc cggccggcgc aggtccgcgg agaccagcac
    18121 gacgtcccgc ccgatctcgg ccagggccgc ccccaggttg accagggtgg tggtcttgcc
    18181 ctcgccccga tcggcgctgg tgaccaggat cgtcttcatg ttcgagggtt ccaggatcga
    18241 gagcaggttg gtccgtagtc gccgataggc atcggcggcg ggcgtgcgca gcccgcgggc
    18301 gaccaccaag gcggcccgct ccggcgtgcc ccaccgggtc cgggggaccg aggcgagcac
    18361 cgggaccccc aggatgccct cgagctcgtt cacgttgcgg accgcgtcgc tgttgcgctc
    18421 gcgcaccagc gcggccccga cccccagtcc cacgcccagc aggatgccca gcgcgatgtc
    18481 gaaggggtgg cgcgggctga tcggcgcggt gggcacgttc gccggatcga tcacctggcc
    18541 gggatcgatg gtcaggttct ggaggttcgc cagcttggag ctgatgatct gggcctgcgc
    18601 tttgtcgaag ctcagcttgc tctgggcggc gggaacatcg aagccggcgg ggggatggtc
    18661 gatcttgccc tggagcttgg cgatcgtggc ctgcaggtcc tcgagctgcg agttggcttg
    18721 accggtctgg gcatcgacgt agtcctgggc ctgctgccga cggtattcca ggtacgcctg
    18781 ggcgaacgcg tcggcccctc gcgcggcagc ggtcggctcc gaggcggtga aggtgatcct
    18841 gaggatctgg gtgccctgcg tcatgtccgc ggacacgtgc ttcaacagct cctgcgtggg
    18901 gacggacgtc ccgagggcgg acttggccag ttccgccacc ttggtcgagg tggccacgtt
    18961 cgtctcggtc tgggcgctga tcgtcgacgg gttcacggtg accccgatcg cgaccagcgg
    19021 gggcttca
//
LOCUS       DASQQQ010000327         3730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_235576, whole genome shotgun sequence.
ACCESSION   DASQQQ010000327 DASQQQ010000000
VERSION     DASQQQ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235576"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VE646_12290"
     CDS             <1..182
                     /locus_tag="VE646_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZD80802.1"
                     /translation="GVVVLVQLYVSIHVTRWVTLLGIVFVLTVLFAREGLWGTFLKLP
                     RRIARMRRRRSFPVA"
     gene            241..1605
                     /locus_tag="VE646_12295"
     CDS             241..1605
                     /locus_tag="VE646_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HZD80803.1"
                     /translation="MSELDLGVLGAVVVTPSGRARRHLYVRQGRVAALTPERLDASRT
                     VDATGLYLLPGMVDTHVHLMDPGPTEREDFPTGTAAAAANGVTTIVEHTHAHPVRTVR
                     DLDEKRRYLRGRAHVDHGLAAHVWPDQIGRLGELWAAGAAFFKIFTCTTHGVPGLDAA
                     RLLAALEDLSRAGATALIHCEDESLTEEAGRALREAGRQDGAVLFEWRSREAELVAAQ
                     TAALLTGITGARATIAHVSHPAVADRVAAERAAGADVAAEACPQYFLLREHEVLDKGA
                     FRKFTPPARARTGADEEAMWARLRGGVLTHVSTDHAPATREQKGRGIWEAEFGLPGLD
                     TTLRLLIDTALRGGLALEDVVRVYAERPARRYGLWPRKGTLAVGADADLVLVDPEPTS
                     VLRDEEVISKAGWTPYAGRQVRGAIRSTYLRGELLAEDRRLVRGLGGRFLPGPGAPTP
                     SDQE"
     gene            1611..2669
                     /locus_tag="VE646_12300"
     CDS             1611..2669
                     /locus_tag="VE646_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZD80804.1"
                     /translation="MPTDEPTAQPRRGPVIDIHCHVYTPACEPLVRDLPALEAEPFDH
                     FGGPSTEYNRRHFAAIGPQLTTPDVRLADMDRMGVDVQAISVAPPQYYYRAPGRLGRR
                     LARVQNDHLAEIVAGHPDRFVGLGTVPLQDPEAAVAELGYVAGELAFRGIEICSNVAG
                     EDLDDPRFLPFWEEVQRLGLLVVIHPHGFTQGDRLTDYYLINVIGNPLDSTVAVARLV
                     FGGVLERYPALRVCVVHGGGYLPFYPYRMDHAWKVRPESRQRIPHPPSTYLARLYFDT
                     VVFDPGQLASLVRWAGADHVLLGTDYPYDMGESDPVRLVSQVPGLDPAGRDAILGGNA
                     ARLLELAPHREMDGGRRR"
     gene            2666..3106
                     /locus_tag="VE646_12305"
     CDS             2666..3106
                     /locus_tag="VE646_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZD80805.1"
                     /translation="MNLTLKAADTIADGALERGRELDCDPLAVAVLDAGGHLKVLKRQ
                     DGAGILRGEIAAGKAWGALGMGLPSRELDRRARERPEFFGALASLAGGRVLPAAGGVL
                     VLSADGEVIGAVGVSGDTSDRDEDCALRGIAAAGLSSNPPLGQS"
     gene            3158..>3730
                     /locus_tag="VE646_12310"
     CDS             3158..>3730
                     /locus_tag="VE646_12310"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HZD80806.1"
                     /translation="MSRYARGQFNVGYEQRVDRRALCRRRVEKAQAERAAAGLDALLV
                     WKDENVRYLTDLRAQLIAGKTTALNGALLVEGEDPILFCSGGERDRVDRTMPWLREVH
                     TIPIVEQRELIAGCVREILAPSLREHGLAEAAVGVDQASTVLLQELRAALPKLALEDG
                     DTPMQRARTIKLPGEIELLEEATALADAVTA"
BASE COUNT          491 a         1372 c         1389 g          478 t
ORIGIN      
        1 cgggcgtggt ggtgctcgtg cagctctacg tgagcatcca cgtgacccgg tgggtgaccc
       61 tgctcgggat cgtcttcgtg ctgaccgtgc tgttcgcccg cgagggcctg tggggcacct
      121 tcctcaagct cccacgcagg atcgcccgga tgcgccggcg gcggtccttc ccggtggcgt
      181 gagggcccgg gccccggtgg accgcgcccc gatgtccgta tcctggagag gagccgcggc
      241 gtgagcgagc tcgacctggg cgtcctggga gccgtggtgg tgaccccctc gggccgcgcc
      301 cggcggcacc tctacgtgcg ccagggacgg gtggcggcgc tcacccccga gcggctggac
      361 gcctcccgga ccgtggacgc caccggcctg tacctgctgc ccgggatggt ggacactcat
      421 gtccacctga tggacccggg cccgaccgag cgggaggact tccccaccgg caccgccgcc
      481 gccgcggcca acggggtgac caccatcgtc gagcacacgc acgcccaccc cgtccgcacg
      541 gtgcgggatc tcgacgagaa gcgccgctac ctgcggggcc gggcccacgt ggaccacggc
      601 ctggcggccc acgtctggcc ggaccagatc ggcaggctcg gcgagctctg ggcggccggg
      661 gccgcgttct tcaagatctt cacctgcacc acccacggcg tccctggcct tgacgccgcc
      721 cgcctgctgg ccgcgctgga ggacctctcc cgagccggcg cgaccgcgct gatccactgc
      781 gaggacgaga gcctcaccga ggaggccgga cgggccctcc gggaggccgg tcgccaggac
      841 ggcgcggtgc tcttcgagtg gcggtcgcgg gaggccgagc tggtcgccgc ccagaccgcc
      901 gcgctgctga ccggcatcac cggggcccgc gccacgatcg cccacgtgag ccatccggcg
      961 gtggcggacc gggtcgcggc cgagcgcgcg gccggggccg acgtggccgc cgaggcctgt
     1021 ccccagtact tcctgctgcg ggagcacgag gtgctggaca agggagcgtt ccgcaagttc
     1081 accccgccgg cgcgagcccg gaccggcgcc gacgaggagg ccatgtgggc ccggctgcgc
     1141 ggcggggtgc tcacgcacgt gtcgaccgac cacgccccgg ccacccgcga gcagaagggc
     1201 cggggcatct gggaggccga gttcggcctc ccggggctgg acaccacgct ccgcctgctg
     1261 atcgacacgg cgctccgcgg cgggctcgct ctcgaggacg tggtccgggt gtacgcggag
     1321 cgcccggccc gccgctacgg tctgtggccg cggaaaggaa ccctcgcggt gggggccgac
     1381 gcggacctgg tcctggtcga ccccgagccc acctccgtcc tcagggacga ggaggtcatc
     1441 tcgaaggcgg ggtggacacc atacgcgggc cgccaggtcc gcggcgcgat ccgctccacc
     1501 tacctgcggg gagagctcct cgcggaggac cggcggctgg tgcgggggct gggcggccgg
     1561 ttccttcccg gtcccggagc cccaacaccc agcgaccagg agtgatgccc gtgccaaccg
     1621 acgagccgac cgcccagccc cggcggggac ccgtcatcga catccattgc cacgtctaca
     1681 ccccggcctg tgagccgctg gtgcgcgacc ttccggccct ggaggccgag ccgttcgacc
     1741 acttcggagg gcccagcacc gagtacaacc ggcggcactt cgccgccatc ggccctcagc
     1801 tcaccacccc ggacgtccgg ctggccgaca tggaccggat gggggtcgac gtccaggcca
     1861 tctcggtggc gccaccgcag tactactacc gggcgccggg ccggctcgga cggcggctgg
     1921 cccgggtgca gaacgaccac ctggcggaga tcgtggcggg gcaccccgac cggttcgtgg
     1981 gcctgggcac cgtaccgctc caggatccgg aggccgccgt cgccgagctc ggctacgtgg
     2041 ccggcgagct ggccttccgg gggatcgaga tctgcagcaa cgtggccggg gaggacctgg
     2101 acgacccccg gttcctgccc ttctgggagg aggtccagcg gctcgggctg ctggtggtga
     2161 tccaccccca cggtttcact cagggggacc ggctgaccga ctactatctg atcaacgtga
     2221 tcggcaaccc gctggactcc acggtggccg tggcgcggct ggtcttcggc ggcgtgctcg
     2281 agcggtaccc ggcgctgcgg gtctgcgtgg tccacggcgg cggctacctc cccttctacc
     2341 cctaccggat ggaccacgcc tggaaggtcc ggcccgagag caggcagcgc atcccccacc
     2401 ctcccagcac ctacctggcc aggctgtact tcgacacggt ggtcttcgat cccgggcagc
     2461 tcgccagcct ggtgcgctgg gccggggccg atcacgtcct gctgggcacc gactacccct
     2521 acgacatggg cgagagcgat cccgtgcggc tggtcagcca ggtacccggg ctggaccccg
     2581 ccggccggga cgcgatcctt ggaggcaacg cggcgcgcct gctcgagctc gcgccgcacc
     2641 gggagatgga tggaggacga cgccgatgaa cctgaccctg aaggccgctg acaccatcgc
     2701 ggacggcgcc ctggagcggg ggcgggagct ggactgcgac ccgctcgccg tcgccgtgct
     2761 cgacgcgggt gggcacctca aggtcctcaa gcgccaggac ggcgccggca tcctgcgggg
     2821 tgagatcgcc gccggcaagg cctggggggc gctggggatg ggcctgccct cccgggagct
     2881 ggaccggcgg gccagggagc gccccgagtt cttcggcgcg ctcgcctcgc tggcgggtgg
     2941 gcgggtgctg cccgccgccg gcggggtcct ggtgctctcc gcggacggcg aggtcatcgg
     3001 cgcggtgggc gtgagcggcg acacctccga ccgggacgag gactgcgcgc tgcggggcat
     3061 cgccgcggcg ggcctgtcct ccaacccgcc gctgggccag tcctgagggc ccgggggtca
     3121 cgaaccagcc agaccgccgg gacggcgagg agggcgcgtg agccggtacg cgcgtggaca
     3181 gttcaacgtc ggctacgagc agcgcgtcga ccgccgcgcg ctgtgccggc ggcgcgtcga
     3241 gaaggcgcag gcggagcgcg ccgccgcggg actggacgcg ctgctggtct ggaaggacga
     3301 gaacgtccgc tacctcaccg acctgcgggc ccagctcatc gccggcaaga ccaccgccct
     3361 gaacggggcg ctcctggtgg agggcgagga cccgatcctg ttctgctcgg gcggcgagcg
     3421 cgaccgcgtc gaccgcacca tgccgtggct acgggaggtc cacaccatcc ccatcgtcga
     3481 gcagcgtgag ctgatcgccg gctgtgtgcg cgagatcctg gcgccgtcgc tgcgcgagca
     3541 cggcctggcg gaggcggcgg tcggcgtcga ccaggccagc accgtcctgc tccaggagct
     3601 gcgcgccgcc ctgcccaagc tggccctgga ggacggcgac acgccgatgc agcgcgcccg
     3661 gacgatcaag ctgcccggtg agatcgagct gctggaggag gccaccgcgc tggccgacgc
     3721 ggtgaccgcc
//
LOCUS       DASQQQ010000328         2619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_50753, whole genome shotgun sequence.
ACCESSION   DASQQQ010000328 DASQQQ010000000
VERSION     DASQQQ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50753"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VE646_12315"
     CDS             <1..345
                     /locus_tag="VE646_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80807.1"
                     /translation="HRLLYVGIPRSRFDRLNELLRERGMPPMDPAQPFTPRPVPDGDI
                     AVSVDCRGVFDAKRAALAEHRTQAEMHDVPFEAWSEMIGWEDFVQAWPERRPDQPLLT
                     DVFAGLPPGELV"
     gene            349..1554
                     /locus_tag="VE646_12320"
     CDS             349..1554
                     /locus_tag="VE646_12320"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZD80808.1"
                     /translation="MQIRGIGSGEFERYATTLETAFGSHVTEAELQQERSVAEIERCL
                     VALAGDEMVGGAAAASFILTVPGAEVPAAGVTGVGVLPTHRRRGVNSALMRRQLDDVH
                     AGPEPVAILYASEGGIYGRYGYGLASYMCSMDIEAERTAFLPGFRAQGVVELLERDRA
                     LADFLPVHEAVRRTRPGMLALDDRWMAYRFGETHHGEERKLFFAAHRSGRGIDGYAVY
                     QVKHDWHIERRNELAVEDVQALTAQAYAELWRYLFDVDLMDRITAWNRPSDEPLLHRL
                     QEPDRLRLRLGDGLWVRLVDLPAALRARRYAVPGSIAFRVEDGFCPWNEGTYRLEGGP
                     DGAECRPTGAEPELALSAAELGACYLGGMGFRALARAGRVRELRPGALERADAMFAWD
                     PAPWCSFMF"
     gene            1628..2077
                     /locus_tag="VE646_12325"
     CDS             1628..2077
                     /locus_tag="VE646_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80809.1"
                     /translation="MKPTVRLAALGVAIALAAACTGSGGGSSDRPTPATTTPAGSPVA
                     AVTGPPGAALYHYANTGLDATIQFEGDTGTLTIQNGTGHELAAPGFYILDARDGTRIK
                     GTVRDPAPVPDGETQEFTVSFKGLEDKNIGLVVLMIGSDNYGAFAPQ"
     gene            complement(2079..>2619)
                     /locus_tag="VE646_12330"
     CDS             complement(2079..>2619)
                     /locus_tag="VE646_12330"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZD80810.1"
                     /translation="RIYTKTGDDGTTGLLYGSRISKADPAAEAYGTADEAVAALGLAR
                     ALCEDPAVAEELLALQRSLFVVGADLATNPEARGRLEPEVSLVTDGMVEALEARIDRL
                     VADHPLPNAFVVPGAAPLSAAIDVARAVVRRAERRVVELRETGTEVNGCVLRYLNRLS
                     DLLFVLARWQAGEAEPRSR"
BASE COUNT          337 a          976 c          958 g          348 t
ORIGIN      
        1 caccggctgc tgtacgtggg gatcccccgg tcccggttcg accgcctgaa cgagctgctg
       61 cgcgagcggg ggatgccgcc catggatccc gcccagccct tcaccccccg gccggtgccg
      121 gacggggaca tcgcggtctc ggtggactgc cggggcgtgt tcgatgccaa gcgcgcggcg
      181 ctggccgagc accggaccca ggccgagatg cacgacgtgc ccttcgaagc ctggtccgag
      241 atgatcggct gggaggactt cgtgcaggcc tggcccgagc ggaggccgga ccagcccctg
      301 ctcacggacg tcttcgcggg cctgcccccg ggggagctgg tctgagcgat gcagatccgc
      361 gggatcgggt cgggggagtt cgagcggtac gccaccaccc tggagaccgc cttcggctcc
      421 cacgtcaccg aggccgagct gcagcaggag cgctcggtgg ccgagatcga gcgctgcctg
      481 gtggccctcg ccggcgacga gatggtgggc ggcgccgccg cggcgtcctt catcctgacc
      541 gtccccggcg ccgaggtgcc cgcggccggc gtcaccgggg tgggcgtgct gcccacccac
      601 cgccgccggg gggtcaactc ggcgctcatg cgccgacagc tggacgacgt ccacgccggc
      661 cccgagccgg tggcgatcct gtacgcctcc gagggcggca tctacggccg gtacggctac
      721 gggctggcct cctacatgtg ctcgatggac atcgaggccg agcgcaccgc gttcctgccc
      781 ggcttccggg cccagggcgt ggtggagctg ctggagcggg accgcgccct ggccgacttc
      841 ctgccggtgc acgaggcggt ccggcgcacc cgccccggca tgctggccct ggacgatcgg
      901 tggatggcgt accggttcgg cgagacccac cacggggagg agcggaagct gttcttcgcc
      961 gcgcaccgct ccgggcgggg catcgacggc tacgccgtgt accaggtcaa gcacgactgg
     1021 cacatcgagc ggcggaacga gctggccgtg gaggacgtgc aggccctgac cgcccaggcc
     1081 tacgcggagc tgtggcgcta cctgttcgac gtggacctga tggaccggat caccgcctgg
     1141 aaccggccgt ccgacgagcc gctgctgcac cggctccaag agcccgaccg cctgcgcctg
     1201 cggctggggg acgggctgtg ggttcgcctg gtggacctcc cggccgccct gcgggcccgg
     1261 cggtacgccg tccccggctc gatcgcgttc cgggtggagg acgggttctg cccctggaac
     1321 gagggcacgt accggctgga gggcggcccc gacggcgccg agtgccgtcc caccggggcc
     1381 gagcccgagc tggccctcag cgcggccgag ctcggcgcgt gctacctggg cgggatgggc
     1441 ttccgcgccc tggcccgcgc cggtcgggtc cgggagctgc ggcccggcgc cctggagcgg
     1501 gccgacgcca tgttcgcctg ggatccggcg ccctggtgct cgttcatgtt ctgatcgggg
     1561 ccacctcggg agcccagggt cccgcccggg gccgggtgcg gtccgttctc gtatgatgcc
     1621 ccaccgcatg aagcctacgg tccgcctggc cgccctgggg gtggcgatcg ccctcgccgc
     1681 cgcgtgcacc ggatcgggcg gtgggtcctc ggaccgcccc acgcccgcca cgaccacgcc
     1741 ggcggggagc ccggtggccg cggtgaccgg acctcccggc gcggcgctat accactacgc
     1801 gaacaccggc cttgacgcca cgatccagtt cgagggcgac accggcaccc tgacgatcca
     1861 gaacggcacc gggcacgagc tggcggcacc gggcttctac atcctggacg cgcgggacgg
     1921 cacgcggatc aagggcacgg tccgggaccc cgcccccgtc cccgacggcg agacccagga
     1981 gttcaccgtg tccttcaagg ggctggagga caagaacatc ggcctggtgg tgctgatgat
     2041 cggctcggac aactacgggg cgttcgcgcc ccagtaggtc accggctgcg cggctcggcc
     2101 tcgcccgcct gccaccgcgc caggacgaac agcaggtcgg acagccggtt caggtaccgg
     2161 aggacgcagc cgttcacctc ggtcccggtc tcgcggagct ccaccacccg tcgctcggcc
     2221 cggcggacca cggcccgggc cacgtcgatc gcagccgaga ggggagccgc gcccggcacc
     2281 acgaaggcgt tcggcaacgg gtgatcggcc accagccggt cgatccgagc ctcgagcgcc
     2341 tcgaccatgc cgtcggtcac cagcgagacc tccggctcca gcctgccgcg ggcctcgggg
     2401 ttggtggcca ggtccgcccc caccacgaac agcgatcgct gcagggcgag gagttcctcg
     2461 gccaccgcgg gatcctcgca cagcgcccgg gccagcccca gggcggccac ggcctcgtcc
     2521 gcggtcccgt acgcctcggc cgccggatcg gccttggaga tccgcgatcc gtacagcagg
     2581 cccgtggtgc cgtcgtcccc ggtcttggtg tagatccgc
//
LOCUS       DASQQQ010000329        11801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_235760, whole genome shotgun sequence.
ACCESSION   DASQQQ010000329 DASQQQ010000000
VERSION     DASQQQ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11801
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235760"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..767)
                     /locus_tag="VE646_12335"
                     /pseudo
     CDS             complement(246..767)
                     /locus_tag="VE646_12335"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(834..1604)
                     /locus_tag="VE646_12340"
     CDS             complement(834..1604)
                     /locus_tag="VE646_12340"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZD80811.1"
                     /translation="MLLQGKRAVVTGASSGIGAATSERLAREGASVLINYYGEQDRGD
                     AEAIAAKIEQAGGSAVPWQTDVGDEDQVEAMIRGAVSRLGGLDLLVNNAGIEKQQPIL
                     EMTLDLWERVLRTDLTGAFLCLREAGKVMAGGGGGVIVNVSSIHEFVPWPGFAHYCAS
                     KGGLKLLTQTAARELAPKGIRVASVAPGAIATPINTSVLHDPEALHAVVDEIPLGRIG
                     RPEEIAAAIAWLASDEAAYATGATFVVDGGMSLYPKFV"
     gene            complement(1738..3465)
                     /locus_tag="VE646_12345"
     CDS             complement(1738..3465)
                     /locus_tag="VE646_12345"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZD80812.1"
                     /translation="MGRATRRAPQPAAPEHAVLRPGETVAALTARYQALVEQIPAVLY
                     INLADESESTIYVSPQTEPILGIAPDAWFSGTWSDHVHPDDRAQVDENYQAFLRSARE
                     GVDEYRFIRPDGREIWIHDRVSIIRDAAGAPILVQGVMFDITEQKRAQAIIAQQAELM
                     GKMDAVSRRFTDLVLSGAELRRVLDTLAAIVGNPVVLEDAAHQLVEYAERSLDLGELL
                     RRWDTHSRSGHPRTGTGGVMEERTADPPCAWISVRFRQEEWGRIHLLQAEGTIQEIDR
                     LALDRAAAAIGLSLLTERDAARSADTARSAFLSDVWRGRWVSAHEVIARARSLGAELQ
                     GRSMVAIVVEVGDPPQPATTHSDPAERRRTREVILDAIREAVGSSNVAGLTAIVGERL
                     VGILGLPGGEDRATLDHIGRTALGRIADRLPGPATVIGVSRRATPETLQRALTEASEA
                     AAYGMRVADRPGIHHFEDLGLLQLLVRLSDGPELPRFVESELGPLLERDAAGRTPLLQ
                     TLRTFLESGGHKAAAARALHLERRSLYYRLENIEHLLGRGLDDAATRLRLEVAFQGLD
                     LLQQRAART"
     gene            3661..5199
                     /locus_tag="VE646_12350"
     CDS             3661..5199
                     /locus_tag="VE646_12350"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HZD80813.1"
                     /translation="MLEARTEDPPSFEYSDALPLHVQADLLVIPTFRGSPPEPGTQDL
                     GLDDAYALANLTGEEGEDLLLVRRPGESFRAGAVLLVGLGEPERVNLDAIRRGAQRAG
                     AKARRFGTVATTLPQACPGAPELAVRATIEGFSTGIYRFAAYRTTDGASDGRTPERIA
                     LLGRPEWAEPEIRMAVDAAAAVERAIAWAKTLVDTPAAELTPARLAERASEMAGRHGL
                     GLRVLDEVDLAEGGFGGILGVGAASAHPPRLIELRYRPPTGVAGTSIALTGKGVTFDA
                     GGLALKKLSEMVGMKSDMAGAAAVLAAMRAIAELAPPDVEVLAAIPTAENMPGGSALR
                     PGDVLRHRNGRCTEVVDPDAEGRLLLADALAYLAESRPAALVDAATLTYSCIAALGDQ
                     ITAAMGNDRAVMDAILRAGEAVGEPMWELPLWQRYRTQLGSGIADSRNEGEEGVAGAI
                     VAGLFLEPFVSGIPWVHLDIGGSGYRDEPSEPLPAGATGVPTRTLVRFVTEYGLERRA
                     EGGS"
     gene            5250..6950
                     /locus_tag="VE646_12355"
     CDS             5250..6950
                     /locus_tag="VE646_12355"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZD80814.1"
                     /translation="MRDGARSPIRLVAPLVLILALVASACSSSGGKGEQTSNGPIKEG
                     GVLRIGTSSGMISPNPFVGFNQDDYSAWMYIYPSLLQYDTTSSSYDYMASFATNWNQS
                     PDGLTVTFDTVPDAKWSDGQALTAEDAAWTFNMIKQYASGPTSSWAGSVTFLKSVTAT
                     DPNTLVATYDRPSGTALFDLGLTPILPPQVWQRYATGNGKELKTFANVPENGQPLVGG
                     GPFVMTQYKKNDIALFQRNDNWYGTKPHIDGFGLQFYRDEDAMITALKSGQLDAINEI
                     PPTSVDTLKSAGMKVYEGQALALRDFIFNVDPHKTEHRELLNPKVREAMEYAIDRSSI
                     ADTAWLGYASPGSTIIPAGNATGGVQWHDPSVQPLPFDIAKANQILDSLGYQRGSDGI
                     RVADGQPMSYQVVFPQDEEGAGDRAFQIIQQDFKQIGVQLAQKRLDDNAAWNAMYSGG
                     HYVFDIAMWDWFPAADPDFILSVLTCDQYGNWNDTGYCNPTYDKLYKDQKAAVDPQQR
                     QKIIFQMQEMVYKDRPYIILTYDKRLDAWSPKWVGLVESTQGIFNNFSTQSLTSVHQA
                     "
     gene            6994..7956
                     /locus_tag="VE646_12360"
     CDS             6994..7956
                     /locus_tag="VE646_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD80815.1"
                     /translation="MRRTDYVIKRAVFAIATVFVAITINFFLFRVLPGDAVRNLARVP
                     RASAALRHSLEVQFGLDKPKWEQYLIYLKELGQGNLGISFSNQQPVWSNLRQDLANTI
                     PMVTLGTLIAIIIGVSTGVFSAWRRGTKLDKVSTNLAIAFYSFPTQWLALVLLIYLST
                     WLPSHGRTNDFLFNPTWWQHMKDVLVHMALPSLTLALTLYGEYTLIIRSAMLETLGED
                     YILTARAKGLPQRAIVIGHALRNALLPTITLIALSLGYIVAGAILIETVFSWPGIGNA
                     VYQAVTERDYPMLQGAFLVLTISVVFFNFLADLLYFKLDPRITE"
     gene            7959..8936
                     /locus_tag="VE646_12365"
     CDS             7959..8936
                     /locus_tag="VE646_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD80816.1"
                     /translation="MATRPDLVEGTSAELEATPSSRHRSWLWQVISESRQAKIGLAVL
                     LFFVAIAVLAPWIEPFDVHTRVGPVYAPPSLAHPLGLDDGGIDMLSLLIQGGRISLIV
                     GSAATLVAMVIGGGIGIVSGYFGGWLDVALMRLTDYFLVIPDLPLAIIVAAVWGASLT
                     HLIFVISLLLWTTTARIIRAQVKSVRERVYVKRARSQGASDLRIILRHVLPQVGPLLI
                     ANTVLTVAVAIFDETALAFLGLGDPTSITWGNIIEFAFLRTAISSGAWWAVIPAGLCV
                     AIVIMACFWLGQAIEDALNPRLKVSHLSPWTFRILWTGGSPEARGVDQT"
     gene            8933..9910
                     /locus_tag="VE646_12370"
     CDS             8933..9910
                     /locus_tag="VE646_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019889871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD80817.1"
                     /translation="MSLLEVRNLHVWFDLAGGGRLHAVQDVSFGLDPGDRMGLVGESG
                     CGKTTTVLALLGLLPPSATVSGEVRLDGASLLTGAEDDLRVHRWKDMAMVFQGAMNAL
                     DPVKTVESQIVEPMELHGTATGPDARRRAGELLELVGMPASAATRYPHEFSGGMRQRA
                     AIAMALACEPKVLLADEPTTALDVMVQAQILEMLTELTRELGLALVLVTHDLPVVAQV
                     CERAAVMYAGRIVETGGMEELFHDPDHPYTRLLFAATPDLYGEEEVSSIPGAPPRLDR
                     EIVGCPFRDRCDRAFERCISEAPHLAVVGPGRTAACHLNDEPVRVRGRS"
     gene            9910..10974
                     /locus_tag="VE646_12375"
     CDS             9910..10974
                     /locus_tag="VE646_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014026340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD80818.1"
                     /translation="MDEVRTLEHLRGSGPVVDEPPLLQVSDLHVVYPVRRGLIGALTG
                     APRKRVRAVDGISLSLRSGEMLALVGESGCGKTTTAHTILRLVDPLSGTVMLEGRDIS
                     TLSQRQLHPVRRRVQMIYQDPYESLDPRFRVRQIVEEPMLIHGVGGRRTERHRRVREA
                     LGRVELTPPDMYADRFPHELSGGQRQRVAIAASLVLDPQLLVADEPVSMLDVSVRAGI
                     LQLLDGLRRTGSLGVLMITHDLSTAAHFADRIAVMYLGRIVEEGPARQVVRDPRHPYT
                     KALLSVVPQRDPRVRPEAQVLRGEPPDPVDVPSGCRFHPRCPAVRDGCRTTDPDLRPV
                     PGVDGHRAACILVEAGGTSS"
     gene            11003..>11801
                     /locus_tag="VE646_12380"
     CDS             11003..>11801
                     /locus_tag="VE646_12380"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZD80819.1"
                     /translation="MIANPERLALVGGAVLRPDDRRRAIVDRRSTAVLVEDGRIAAIG
                     DDRTILEHAGSRAEVLDVHGGVIAPGFVDAHIHAFDRALASLKVSCLPPGVDRLAALT
                     RRLHERVTHAAPTDWIVGQGYDDMRLAERRHPTRRDLDQAVGERPAVVTRVCGHMSVA
                     STTALRLAGIDEWTRDPAGGSIVRDAAGSPTGLLLESAQDLVLDLVPPPGIGEISRAL
                     RETGRVLLGHGITTICEALLGAFHPLEHEIWSRVLADGWVGPDVRFLA"
BASE COUNT         1755 a         4060 c         4116 g         1870 t
ORIGIN      
        1 gtacctcacg cccaccgtgg cggcggactt cacgcacggg atcgatgtgg cgctctcggt
       61 ctccgtcgtg atcgtgctgg cggcactcgt cccgatcctg atggggggcg agtcgagggc
      121 cgcacggcgc gcggcgggcc gggtggcagc gaaggcgtga gggaaggcat cgacttgggc
      181 cgggctcgcg gcccgaggtc ccggatagtc gcggataccc gcgcggtcac gccgaggtcg
      241 gccagctacg ggacgaaggc catcacgctg agcggcgatc ccgatccttt cttcagctgc
      301 agcggcccga gcaccagcgt cgcacccgtc ggtggcagct ggtccaggtt ggccaggcac
      361 tcgatcgcga tcttgtgggc ctccgcgatc tgtgtgttgg tcttgtacac cgtgtcgagc
      421 ccaggatcca cgccgtgggt gtcggtcccc acgccggaga tctgccgatg ggcgagcagc
      481 cacctcgtcg tccttcccgc gaaccggggg aagtggaggt tcccacccgc gtcctggttg
      541 atgaacgctt ccggatcgtt ccacatgtct tcccagcccg tgaacaggat catgaacgag
      601 ccgggcgcga tctttccgta cctggcctcc caggcccgga cgccctcctt cgtgagccga
      661 tagtcggggt cctcggcggc ctcgtcgcgc acgtcgatca cgacggcggg gaccactcgc
      721 tgctcggggg ggcagctggt gatggcttcc gtgttgtcgg ggaggaagct gttgggcgcg
      781 cttccagatc ccgcatcatg ccaggcgggc gctcctgcaa ggtcccgacg ggctcacacg
      841 aacttcgggt acagcgacat gccgccgtca acgacgaacg tggcgccggt cgcgtacgcc
      901 gcctcgtcgc tggccagcca ggcgatggca gcggcgatct cctccggccg gccgatccgg
      961 ccgagcggga tctcatccac caccgcgtgc agggcctcgg ggtcgtggag gacggaggtg
     1021 ttgatgggcg tcgcgatggc gccgggggcg acggaggcca cccggatccc cttcggcgcc
     1081 agctcgcgag ccgcggtctg cgtcagcagc ttcaaaccgc ccttgctggc gcagtagtgg
     1141 gcgaaccccg gccaggggac gaactcgtgg atgctcgaga cattgacgat gacgccgcct
     1201 ccgccccccg ccatcacctt gcccgcctcc cgcaggcaca ggaacgcacc ggtcaggtcc
     1261 gtacgaagca cgcgctccca caggtccagg gtcatctcca ggatcggctg ctgcttctcg
     1321 atcccggcgt tgttgaccag caggtcgagt ccgcccaggc gcgagaccgc gccccggatc
     1381 atcgcttcga cctggtcctc gtcaccgacg tcggtctgcc atgggaccgc cgatccgccc
     1441 gcctgttcga tcttggccgc gatcgcctcg gcgtcgccgc gatcctgctc gccgtagtag
     1501 ttgatcagga ccgaggcgcc ctcgcgggcc agccgctcgc tggtcgccgc tccgatccca
     1561 ctcgacgccc cggtcaccac ggcccgtttg ccctgtagca gcatggctcc tcctcgttgt
     1621 cctcgctcca tcgcggcccg atcggccctg cggcccacgg cccgagcagc acctccccgc
     1681 tccctcccgt cctcattcgt cgtggcccag gggagggact tgggttcagg cccgaggtca
     1741 ggtgcgggcg gcgcgctgtt gaaggagatc caggccctgg aaggcgacct ccagccggag
     1801 cctggtcgcg gcatcgtcca ggcccctgcc caggaggtgc tcgatgttct ccagccggta
     1861 gtagagggac cgtcgctcca ggtgcagggc cctcgcggcg gccgccttgt ggccgccact
     1921 ctccaggaag gtccgtaggg tctgcagcag cggagtgcga ccggccgcgt cacgctcgag
     1981 aaggggaccc agctccgact ccacgaaccg cggaagctcg ggcccatcgc tcaggcggac
     2041 gagcagttgg aggaggccca ggtcctcgaa gtgatggatc ccggggcggt cggccacccg
     2101 catgccgtag gctgcggcct cgctcgcctc ggtgagtgcg cgctgcaggg tctcgggcgt
     2161 cgcccggcgg ctcacgccga tcaccgtggc cggtccgggg agtcgatccg cgatgcgacc
     2221 gagcgccgtc cgaccgatgt gatcgagcgt cgcccggtcc tcacccccgg gcaggcccag
     2281 gatcccgacc aggcgctcac ccacgatggc cgtgaggccg gcgacgttcg aggaccccac
     2341 ggcctcccgg atcgcgtcca ggatcacctc gcgggtgcga cgacgctcgg ccggatccga
     2401 gtgggtggtc gccggctggg gcgggtcgcc cacctcgacc acgatcgcca ccatgctgcg
     2461 gccctgcagc tcggcaccga ggctccgcgc tcgagcgatg acctcgtggg ccgacaccca
     2521 ccgtccccgc cagacgtccg agaggaacgc ggagcgggcg gtgtccgcgg agcgtgcagc
     2581 atcgcgctcg gtgagcaacg acaggccgat ggcggctgcg gcgcggtcca gggcgaggcg
     2641 atcgatctcc tggatcgtcc cctcggcctg caggagatgg atcctgcccc actcctcctg
     2701 ccggaaccgg acggagatcc aggcacaggg tgggtcggcg gtccgctcct ccatcacgcc
     2761 ccccgttccc gtgcgaggat ggcccgaccg cgagtgcgtg tcccagcgcc gcagcagttc
     2821 ccccagatcc agtgagcgct ccgcgtactc caccagctgg tgggccgcat cctccaggac
     2881 gaccgggttc cccacgatgg ctgccagcgt gtcgagcacg cgcctcagtt ccgcgccgct
     2941 cagcaccaga tccgtgaacc gcctgctgac ggcatccatc ttccccatca gctccgcctg
     3001 ctgggcgatg atcgcttgtg cccgcttctg ttcggtgatg tcgaacatca ccccctgaac
     3061 gaggatcggg gccccggccg cgtcccggat gatcgagaca cggtcgtgga tccagatctc
     3121 ccgaccgtcg ggccggatga accggtactc gtccacgcct tcccgagcgc tccgaaggaa
     3181 ggcctggtag ttctcgtcga cctgggctcg atcatccggg tggacgtggt cgctccaggt
     3241 tccggagaac cacgcgtccg gtgcgatccc caggatcggc tcggtctgcg ggctcacgta
     3301 gatcgtcgac tcggactcgt cggccaggtt gatgtacagc acggccggga tctgctcgac
     3361 cagggcttgg tacctagcgg tcagggctgc aacggtctca cctggtcgaa gcacggcatg
     3421 ctcgggcgcg gcgggctggg gcgctcggcg cgtggctcga cccatgaagg cccaagcata
     3481 gggcatcgcg ctctgtgcaa gcgatgaccg gagcctttct cggagcgtgt catgaccgag
     3541 gggcgccggt gtggtcccgt aggcctcgat cagcgcaccg gggccagacg gccgcgagtt
     3601 ggggggttct gcgtgagcgg aggagccggg gggccagcct tatcccgatc tgcgcctccg
     3661 atgctcgagg cgaggacaga agacccgccc tcgttcgagt actcggacgc cctgcccctc
     3721 cacgtccagg ccgatctcct cgtgatcccg accttccgag gatctccccc tgaaccgggc
     3781 acgcaggacc tgggtctgga cgatgcctat gccctggcga acctcaccgg cgaggagggc
     3841 gaggacctac tcctggtccg ccgaccgggc gagtcgttcc gagccggagc ggtcctgctg
     3901 gtggggttgg gcgagcccga gagggtcaat ctggatgcga tccgccgcgg ggcgcagcgg
     3961 gccggagcga aggcgaggag gttcggcacc gtggcgacca ccctgcccca ggcgtgtccc
     4021 ggcgcacccg agctcgccgt gcgagcgacg atcgaaggct tctccaccgg gatctaccgg
     4081 ttcgccgcct accggacgac cgacggcgcg tcggacggcc ggaccccgga acggatcgcg
     4141 ctgctcggca ggcccgagtg ggcggaaccg gagatccgga tggcggtgga cgcggccgcc
     4201 gcggtcgaac gggccatcgc gtgggcgaag accttggtgg acacgccggc cgcggagctc
     4261 acgcccgctc gcctggcgga acgagcgagc gagatggccg gcaggcacgg actcggactc
     4321 cgggtcctgg acgaggtgga cctggcggag ggggggttcg gggggatcct cggtgtggga
     4381 gccgcctcgg ctcatccgcc gaggctgatc gagctccggt accgaccgcc cacgggggtc
     4441 gccggtacat cgatcgccct gaccgggaag ggcgtcacct tcgacgccgg gggcctcgcg
     4501 ctgaagaagc tctcggagat ggtcgggatg aagtcggaca tggcgggagc ggcggcggtc
     4561 ctggcggcga tgcgggcgat cgcggagctc gcaccaccgg acgtcgaggt gctcgcggcc
     4621 atcccgaccg cggagaacat gccgggcggc tccgccctgc ggccgggcga cgtgctccga
     4681 cacaggaacg gcaggtgcac cgaggtggtc gaccccgacg ccgaggggcg tctgttgctg
     4741 gcggacgccc tcgcgtacct ggccgagtcc cggccggccg ccctggtcga cgcagccacc
     4801 ctcacctact cctgcatcgc cgcgctgggg gatcagatca ccgcagcgat ggggaacgac
     4861 cgagccgtga tggatgcgat cctgcgggcc ggggaagccg tcggcgagcc catgtgggag
     4921 ctccccttgt ggcagcgcta ccggacccag ctcggttccg ggatcgccga cagccgcaac
     4981 gagggggagg agggtgtggc gggcgcgatc gtcgccggcc tcttcctcga gccgttcgtc
     5041 tccggcatcc cgtgggtgca cctggacatc ggtggcagcg ggtaccggga cgagccctcg
     5101 gagccgctgc cggccggcgc caccggggtg cctacgcgca cgctggttcg gttcgtcacg
     5161 gagtacggcc tggagcgccg ggctgagggg gggtcgtgat ggttggagcg aggaggcagt
     5221 cgccgatgac agtccgaggg ggagggatca tgagggatgg agcgcgttcg ccgatccgcc
     5281 tggtcgcgcc gctggtgctg atcctggcgc tggtcgcctc ggcctgcagc agctcgggcg
     5341 ggaaggggga gcagacgagc aacggaccga tcaaggaggg cggggtcctg cggatcggaa
     5401 ccagttcggg catgatctcc ccgaacccgt tcgtcggctt caaccaggac gactactcgg
     5461 cgtggatgta catctacccg agcctgctcc agtacgacac cacgtcctcg agctacgact
     5521 acatggccag cttcgcgacg aactggaacc agtccccgga cggcctgacc gtgacgttcg
     5581 acacggtgcc cgatgcgaag tggtccgatg gacaggccct cacggcggag gatgccgcct
     5641 ggaccttcaa catgatcaag cagtacgcga gcggcccaac cagctcctgg gccggcagcg
     5701 tcacgttcct gaagagcgtg acggccaccg atcccaacac cctggtcgcg acgtacgacc
     5761 ggccctcggg gacggcgctg ttcgacctcg ggctgacccc gatcctgccg ccgcaggtgt
     5821 ggcagcggta cgccacgggc aacggcaagg agctgaagac cttcgccaac gtcccggaga
     5881 acggccagcc gttggtgggc ggcgggccct tcgtgatgac ccagtacaag aagaacgaca
     5941 tcgcgctctt ccagcgcaac gacaactggt acgggaccaa acctcacatc gacggcttcg
     6001 gcctgcagtt ctatcgggac gaggacgcca tgatcaccgc gctgaagagc gggcagctcg
     6061 acgcgatcaa cgagatcccg cccaccagcg tcgacacgtt gaagtcggcg gggatgaagg
     6121 tgtacgaggg gcaggccctc gccctccgcg acttcatctt caacgtcgac ccgcacaaga
     6181 ccgagcaccg cgagctcttg aaccccaagg tgcgggaggc gatggagtac gcgatcgacc
     6241 ggtcgtcgat cgccgatacg gcctggctcg gctacgcgtc gcccggttcc acgatcatcc
     6301 cggccggcaa cgccaccggc ggggtccagt ggcacgaccc gagcgtccaa ccgctgccct
     6361 tcgacatcgc caaggccaac cagatcctcg actcgctggg gtaccagcgg ggatcggacg
     6421 ggatccgggt ggccgacggt cagcccatgt cctaccaggt ggtcttcccc caggacgagg
     6481 agggggccgg ggaccgcgcc ttccagatca tccagcagga cttcaagcag atcggcgtcc
     6541 agctggcgca gaagcggttg gacgacaacg cggcctggaa cgcgatgtac tcgggtgggc
     6601 actacgtctt cgacatcgcg atgtgggact ggttcccggc tgcggacccc gacttcatcc
     6661 tgtcggtcct cacctgcgac cagtacggca actggaacga caccggctac tgcaacccga
     6721 cctacgacaa gctctacaag gatcagaagg cggccgtgga tccccagcag cggcagaaga
     6781 tcatcttcca gatgcaggag atggtgtaca aggaccggcc ctacatcatc ctcacctacg
     6841 acaagcggct cgatgcctgg tcgccgaagt gggtcgggct ggtggagtcg acccagggga
     6901 tcttcaacaa cttctccacg cagagcttga cctccgtgca ccaggcgtag gcgatccaca
     6961 cctcggaacg ggaggcggac gaaggacccg gtcgtgcggc gaaccgacta cgtgatcaag
     7021 cgagccgtgt tcgcgatcgc cacggtgttc gtggcgatca cgatcaactt cttcttgttc
     7081 cgcgtgctgc cgggggatgc ggtccgtaac ctggcccgcg tcccccgggc gtccgccgcg
     7141 ctgcgccact cgctcgaggt gcagttcggc ctggacaaac cgaagtggga gcagtacctg
     7201 atctacctca aggagctggg tcaggggaac ctgggcatct ccttctcgaa ccagcagccg
     7261 gtctggtcca acctgcgcca ggacctggcc aacacgatcc cgatggtcac gctcggcacc
     7321 ctgatcgcca tcatcatcgg ggtcagcacc ggcgtcttct ccgcctggcg acgagggacg
     7381 aagctcgaca aggtcagcac caacctggcc atcgccttct actcgttccc cacccagtgg
     7441 ctggccctgg tgctcctgat ctacctctcg acgtggctgc cgagccacgg gcggacgaac
     7501 gacttcctgt tcaaccccac ctggtggcag cacatgaagg acgtcctggt ccacatggcg
     7561 ctgccctccc tcacgctggc cctgaccctg tacggcgagt acacgctgat catccgctcc
     7621 gcgatgctgg agacgctcgg cgaggactac atcctgaccg cccgggcgaa ggggcttccc
     7681 cagcgggcca tcgtgatcgg tcacgcgctg cgcaacgccc tgttgcccac gatcacgctc
     7741 atcgccctgt ccctcggcta catcgtggcc ggggcgatcc tgatcgagac ggtcttctcg
     7801 tggcccggga tcggcaacgc cgtgtaccag gcggtcacgg agcgcgacta cccgatgctg
     7861 cagggggcct tcctcgtgct gaccatctcc gtggtcttct tcaacttcct ggccgatctg
     7921 ctgtacttca agctcgatcc gaggatcacg gagtagcgat ggccacccga cccgacctgg
     7981 tggaaggcac gagcgcggaa ctggaggcga cgccgagcag ccgccatcgt agctggctgt
     8041 ggcaggtcat ctccgagagc cgccaagcca agatcgggct ggccgtcctg ttgttcttcg
     8101 tcgctatcgc cgtgctggct ccatggatcg agccgttcga cgtgcacacg agggtcgggc
     8161 ccgtctatgc gccgccatcg ctggcccatc ccctggggct cgacgacggc gggatcgaca
     8221 tgctgtcctt gctgatccag gggggacgca tctcgctgat cgtggggtcg gccgcgacgt
     8281 tggtggccat ggtgatcggc gggggtatcg ggatcgtgtc cgggtacttc gggggctggc
     8341 tggacgtcgc cctgatgcgg ctcacggact acttcctggt gatcccggac ctgccgctcg
     8401 ccatcatcgt cgctgccgtc tggggcgcca gcctcaccca cctgatcttc gtgatctcgt
     8461 tgctcctgtg gaccaccacg gcccggatca tccgggccca ggtcaagagc gtccgcgagc
     8521 gggtgtacgt gaaacgggcg cgatcacagg gtgcgagcga tctgcgcatc atcctgcgcc
     8581 acgtcctccc gcaggtgggg ccgctgctga tcgcgaacac ggtcctcacc gtcgccgtgg
     8641 ccatcttcga cgagacggcg ctcgcgttcc tggggctggg agatccgacc tcgatcacct
     8701 ggggcaacat catcgagttc gcgttcctgc gcaccgcgat ctcctcgggc gcctggtggg
     8761 ccgtcatccc ggccggcctc tgcgtggcca tcgtcatcat ggcctgcttc tggctggggc
     8821 aggcgatcga ggacgccttg aaccctcggc tgaaggtctc ccatctgtcc ccgtggacct
     8881 tccggatcct ctggacggga ggctccccgg aggccagggg ggtcgatcag acgtgagcct
     8941 gctcgaggtc cgcaacctcc acgtctggtt cgacctggcc ggcggagggc gtctgcacgc
     9001 cgtgcaggac gtgagcttcg gcctggaccc cggggatcgg atgggactgg tgggggagtc
     9061 cgggtgcggc aagaccacca cggtcctggc cctgctgggg ctgttgccgc cctcggccac
     9121 ggtgtccgga gaggtccgac tggacggggc gagcctgctg accggtgccg aggacgatct
     9181 ccgggtccac cggtggaagg acatggcgat ggtgttccag ggtgcgatga acgccctgga
     9241 tccggtgaag acggtcgagt cccagatcgt ggagcccatg gagctgcacg ggaccgccac
     9301 gggcccggac gctcgtcgtc gggccggcga gctcctggag ctggtgggca tgccggcctc
     9361 ggccgcaacc cggtaccccc acgagttctc gggtgggatg cgccagcggg cggccatcgc
     9421 catggccctg gcctgtgaac ccaaggtgct gctggccgat gagccgacca ccgcgctcga
     9481 cgtgatggtg caggcgcaga tcctggagat gctcaccgag ctcacccggg agctggggct
     9541 ggccctggtc ctggttacgc acgatctccc ggtggtggcg caggtgtgcg agcgggccgc
     9601 ggtgatgtac gcgggacgga tcgtcgagac cggcgggatg gaggagctct tccacgatcc
     9661 cgatcatccg tacacgcggc tgctgttcgc cgccaccccg gatctgtacg gcgaggagga
     9721 ggtgagctcc atccccgggg ccccccctcg gctggaccgg gagatcgtgg gatgtccctt
     9781 ccgggaccgg tgcgaccggg ccttcgagcg atgtatctcc gaggcgccgc acctggccgt
     9841 ggtagggccg ggccggacgg ccgcgtgtca cctgaacgac gagcccgtcc gggtgcgggg
     9901 gcgatcgtga tggacgaggt ccggaccctc gagcacctgc gcggctccgg tccggtcgtg
     9961 gacgagcccc cgctgcttca ggtgtccgat ctgcacgtcg tgtacccggt gcggcggggc
    10021 ctgatcgggg cgctcaccgg tgcgcctcgg aagcgggtcc gagccgtgga cggcatctcg
    10081 ctctcgctcc gatcgggcga gatgctcgcg ctggtcggcg agtccgggtg cggcaagacg
    10141 accacggcgc acacgatcct gcggctcgtc gaccccctct cgggaaccgt gatgctggag
    10201 ggacgcgaca tcagcaccct ctcgcagcgg cagctccatc cggttcgtcg ccgggtgcag
    10261 atgatctacc aggatccgta cgagtccctg gacccgcggt tccgcgtacg gcagatcgtt
    10321 gaggagccca tgctgatcca cggggtgggc ggcaggcgga ccgaacgcca ccggcgggtc
    10381 cgggaggcgc tgggtcgggt ggagctcacc ccacccgaca tgtacgcgga tcggttcccg
    10441 cacgagctct cgggtggtca gcgccagcgg gtggcgatcg cggccagcct ggtgctggat
    10501 ccccagttgc tggtggccga cgaaccggta tcgatgttgg acgtctcggt ccgggctggg
    10561 atcctccagc tcctcgatgg gctccgccgg acgggcagcc tgggcgtgct gatgatcacg
    10621 cacgacctgt ccaccgcggc acacttcgcc gatcggatcg ccgtgatgta cctggggcgg
    10681 atcgtggagg aggggccggc tcgccaggtg gtcagggatc cccgacatcc gtatacgaaa
    10741 gccctgctgt cggtggtgcc gcaacgtgac cctcgcgtgc gccccgaggc gcaggtcctc
    10801 cggggggagc cgcccgaccc cgtcgacgtc ccctccgggt gccgcttcca cccccggtgc
    10861 cccgcggtgc gggatgggtg ccggaccacc gatcccgatc tgcgccccgt gccgggcgtc
    10921 gatgggcatc gagcggcctg catcctggta gaagcgggcg ggacctcctc gtgagctcgc
    10981 tcggcccccg aacgccggag ccatgatcgc gaacccggag cgcttggccc tggtgggtgg
    11041 ggcggtcctg aggcccgatg accgtcggcg tgcgatcgtg gatcggcggt ccacggcggt
    11101 gctggtggag gacggtcgga tcgccgcgat cggcgacgat cgcaccatcc tggagcacgc
    11161 gggatcgagg gcggaggtgc tggacgtaca cggcggggtg atcgctccgg gcttcgtgga
    11221 cgcccacatc cacgccttcg atcgcgccct ggcgtccctg aaggtctcct gcctgccccc
    11281 cggcgtcgac cgtctcgctg cgctgacgcg ccgcctgcac gaacgagtca cgcacgccgc
    11341 gccgaccgac tggatcgtcg gtcaggggta cgacgacatg cgcctggccg agcgccgcca
    11401 cccgaccagg cgcgatctgg accaggcggt gggggaacgg ccggccgtgg tgacccgggt
    11461 ctgcggacac atgagcgtgg cgagcaccac cgcccttcgg ctcgcgggga tcgacgagtg
    11521 gacccgagac cctgcgggcg gttccatcgt ccgcgatgcc gctgggtcgc ccaccggact
    11581 gctcctggag agcgcgcagg atctggtgct cgacctggtg ccccctccgg ggatcgggga
    11641 gatctcccgc gcgctccgcg agaccgggcg ggtgctgctc ggccacggca tcaccaccat
    11701 ctgcgaggcg ctgctcggcg ccttccatcc cctggagcac gagatctggt cccgcgtgct
    11761 cgccgatggc tgggtgggac cggacgtgcg cttcctggcc g
//
LOCUS       DASQQQ010000330         6856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_301359, whole genome shotgun sequence.
ACCESSION   DASQQQ010000330 DASQQQ010000000
VERSION     DASQQQ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6856
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301359"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VE646_12385"
     CDS             <1..224
                     /locus_tag="VE646_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80820.1"
                     /translation="WPPVPKTIQLTLSEATRLSRAGVTLNVFMLEDSPGLIRFMERLA
                     RLTAGRVFLMDDRDLGAFVVRDYVSRRTR"
     gene            221..805
                     /locus_tag="VE646_12390"
     CDS             221..805
                     /locus_tag="VE646_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80821.1"
                     /translation="MTRRLLAITTLLVLAACTGRVPHGPSRSSPATSPAVSSTDATDE
                     PLSPRRWTGTIVTRSWHDLYVGGRCSSDWRTAISVRERDGALTGSGTAVRTSTGPPCP
                     FPVAQLQIHRFLLRVEGTVSGRQLTLRLHERSTVPSVGADDLGGFRTTTLGTVLHLQV
                     RGGRVVQHLRLEAPDQDRGTFGSSNAVRLRCTGC"
     gene            complement(810..1634)
                     /locus_tag="VE646_12395"
     CDS             complement(810..1634)
                     /locus_tag="VE646_12395"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZD80822.1"
                     /translation="MRDPARRWAAALRAWGIPDRILAQAPEPPWGFPSESFRRRTEQA
                     VSAGPTLTTRRALEALPDRGAVLDVGVGAGAASLPFAPRAARIVGVDASEDLLAAFRT
                     AAEGIDVSVETVSGRWPDVAGQVGVADVAVCAHVLYNVQDVEPFVRALDEHVRRRVVV
                     EITGSHPLAWMNPLWLRFHRLHRPEAPTAGDALAVLRQLGIDATIEREARRSRGGFER
                     REDAVAVVRKRLCLPADRDPEVAEALGPLLRRRDGLWSAGPPEQDIATLWWDRPER"
     gene            complement(1631..2809)
                     /locus_tag="VE646_12400"
     CDS             complement(1631..2809)
                     /locus_tag="VE646_12400"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015373961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZD80823.1"
                     /translation="MLDERLASDGPDLPLSGIRVLDFSRVLAGPLATMILADLGADVV
                     KVERPDVGDDTRGWGPPFVGHDAAYFLSLNRNKRSVTLDLSTDRGVGSARRLVLGADV
                     VIENFRPGLMRSFGLDYQALVGANPGLVYCSLLAFSEEGENAARPGYDIIVQALSGLM
                     SITGQPGGEPTKVGVALLDVICGLYAANGIQAALIGRARTGRGQRITVSLLESSLAAL
                     VNQAANHLLGGVVPGQMGNEHPNIVPYQLFHASDRPFILAAGNDRAFARTCEAIGRPE
                     LSTDPMFATNELRVRHRSELIPILREVFASDRAARWLKALGEAGVPCGPVRSLEEVFT
                     APEGLAMVQTVEDPARGPLRLVANPIRFSGGLLPVRLTPPSLGEHTKQILRPFEDEPR
                     "
     gene            3029..3670
                     /locus_tag="VE646_12405"
     CDS             3029..3670
                     /locus_tag="VE646_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80824.1"
                     /translation="MNKESLDRANAFLAAVAERVEPGGFLEASPAEVGRSMGLSEPLA
                     AARAVRALLARRRLEAVDGRYRLVDPRPIEPGEREQVPRPRRPRAPKARTGEEAPSGR
                     PTYSEVGREAIERLIELGREVANLRAHLRTAREEVRESREARDEAERRAQTLAVRVRE
                     LESRAEMAETNLRTVLAAAKGAGKELPVGDTEMEAILGVLKGGAEEQAGAQTS"
     gene            complement(3673..4791)
                     /locus_tag="VE646_12410"
     CDS             complement(3673..4791)
                     /locus_tag="VE646_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017622412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD80825.1"
                     /translation="MSEIAAEAVRARSEGALPVLEAREVHVHFAGQMGLGSGLLGRKP
                     TVARAVDGVDLELYPGEVVALAGESGCGKTTLARTIMGLEKPTSGEILFEGKPLLSRN
                     LREYRRRVQMVFQDPTAALNPRQTIYESVAEGLRVHGIDRSEDGLAEEGMVARALSRA
                     GLRPPERFFLNYPHELSGGQRQRVVIAGALVLEPEVIIADEPVSSLDASVRGEILRLL
                     LQLRNDLGIAMLVVTHDLGLAWTIADRVAVMYLGRIVEIGDAEDVLTRPMHPYTKALL
                     EVVPEAGGIDRPFLQGEPPDPTLMPPGCRFHPRCPALAEGRAASAGVESRCREEDLGL
                     EQLSEGRFVACHLAARLARGGVPSPPPASEGETEPTKA"
     gene            complement(4788..5771)
                     /locus_tag="VE646_12415"
     CDS             complement(4788..5771)
                     /locus_tag="VE646_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019889871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD80826.1"
                     /translation="MTLLSVRDLQITYHTQAGPVPAVRGVNLEIGRGEVLGLAGESGC
                     GKTTVINAILRLLPPQTRVEGTVLLDGEDVLAMRPGRLRAVRWTQASVVFQGAMHALN
                     PVRRIGAQIAEAALVHGQAGESEAMTRAASLLEQVGLPPRRLRDFPHELSGGQKQRVM
                     IAMALACNPKIVIADEPTTALDVMVQAQVLRLLKDLQRDLGLAMLFITHDLSVLVEVS
                     DKLAIMYAGKIVEEGPSIPVFTDPAHPYTRALAGAFPQIGDRTYRMAPSGLPGDPPFP
                     GDVPSGCSFHPRCPRAFEDCPHIDPELFQAGEARRAACLLVPRSEMAEAEP"
     gene            complement(5768..6736)
                     /locus_tag="VE646_12420"
     CDS             complement(5768..6736)
                     /locus_tag="VE646_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD80827.1"
                     /translation="MRPEIGQVTEPPPPPVGEETVGLPPPTDPRKIARIRRRRALSRV
                     WRIYRGNRMGMAGLYILAFFVLVAVLAPLIASHAQLDPTCTCTGAPFQAPSLKFPFGT
                     DDLGRSVFALTVWGSRISLIVGLTATLISMGIGALIGIAAGYYGGWPETVLMRVTDWF
                     LVLPWLALAIVLAALIGPSLVVIIFVIGITSWPSTARLVRAQTLSVKTRPYVERARAL
                     GASNWHLITRHILPNVGPLIFANTILTVAIAILSETTLSFLGLGDPISISWGTILEFA
                     FNSGAAGAGNWWWLLPPGVCIVLVVLAFTMCGFALDEILNPKLRER"
BASE COUNT         1065 a         2404 c         2433 g          954 t
ORIGIN      
        1 catggccgcc cgttcccaag acgatccagc tcacgctgtc ggaggccacc aggctgtcca
       61 gggcgggcgt cacgctgaac gtcttcatgc tcgaggactc gcccgggcta atccgattca
      121 tggaacggct cgccaggctc acggccggac gggtgttcct gatggacgac cgcgacctcg
      181 gggcgttcgt ggttcgcgat tacgtctctc gtcgcacccg atgacccgtc ggctgctcgc
      241 gatcaccacg ctgctcgtgc tcgctgcgtg caccggacgc gttccccacg gcccgagccg
      301 gtcgtcgccg gccacgtcgc cggcggtctc ctccaccgac gcgacggacg aacccctctc
      361 accccggcgc tggacgggca cgatcgtgac gcgcagctgg cacgacctgt acgtgggagg
      421 acgatgctcg agcgattggc ggaccgccat cagcgtgcga gagcgcgacg gtgcgctcac
      481 gggctccggc acggcggtcc ggacctcgac ggggccgccg tgcccgttcc cggtggcgca
      541 gctgcagatc catcgcttcc tcctccgcgt cgagggaacc gtcagcggtc gccagctgac
      601 cctacgtctg catgaacgct ccaccgtgcc ctcggtcggg gccgacgatc tgggtgggtt
      661 ccgcaccacc accttaggca ccgtgctcca cctgcaggtc cggggtggga gggtggtcca
      721 gcacctccgc ttggaagcgc cggatcagga ccggggtacc ttcggctcct cgaacgccgt
      781 ccgtctccgc tgcaccgggt gctgatcgct caccgttcgg ggcggtccca ccagagggta
      841 gcgatgtcct gctcgggcgg tccggcggac cacaggccgt cgcgtcgccg gagcagcggg
      901 ccgagggcct cggcgacctc gggatcgcga tccgccggta ggcacagccg cttgcggacc
      961 accgccacag cgtcctcgcg gcgctcgaac ccacccctcg agcgacgagc ctcccgctcg
     1021 atcgtggcat cgatcccgag ctgacggagg acggccaggg cgtcccccgc ggtcggtgct
     1081 tccggacgat gcagccgatg gaagcggagc cagagcgggt tcatccaggc cagggggtgc
     1141 gagcccgtga tctccacgac cacccgcctg cggacgtgct catccagggc tcggacgaac
     1201 ggctccacgt cctgcacgtt gtacaggacg tgggcgcaca cggccacgtc ggccacccct
     1261 acctgccccg ccacgtcggg ccagcgaccc gacacggtct ccacggagac gtcgatccct
     1321 tcggccgccg tccggaaggc agccagcagg tcctcggagg cgtcgacgcc cacgatccgc
     1381 gccgcccgcg gggcgaacgg tagcgacgcc gctcccgcgc cgacccccac gtcgagcacc
     1441 gccccgcggt cggggagcgc ctccagggcc cggcgggtgg tgagcgtggg cccggcactc
     1501 acggcctgct cggtgcgtct gcggaacgac tcggagggga agccccacgg cggctcggga
     1561 gcctgggcca ggatccggtc ggggatcccc caggcccgca gggccgcggc ccacctccgc
     1621 gccgggtccc tcaccgaggc tcgtcctcga acggccggag gatctgcttc gtgtgctcgc
     1681 ccaggctcgg gggtgtcagc cgaaccggca ggagcccacc ggagaaccgg atcgggttgg
     1741 ccaccaggcg gagcgggcca cgggccgggt cttccaccgt ctggaccatc gccaaaccct
     1801 ccggggcggt gaagacctcc tccagggatc gcaccggccc gcacgggacc ccggcctcgc
     1861 cgagcgcctt cagccatcgg gccgccctgt ccgaggcgaa gacctcccgc aggatgggga
     1921 tcagctcgga ccggtggcgg acccgcagct cgttcgtggc gaacataggg tcggtcgaca
     1981 gttcagggcg tccgatcgct tcgcaggtcc tggcgaacgc acggtcgttg ccggcggcga
     2041 ggatgaaggg ccgatcggag gcatggaaga gttggtacgg aacgatgttc ggatgctcgt
     2101 tgcccatctg ccccgggacg acgccaccga ggaggtggtt cgcggcctgg ttcacgagcg
     2161 cggccaggct gctctcgagc agcgagacgg tgatccgttg cccccggccg gtccgcgccc
     2221 gcccgatcag cgcggcctgg atcccgttgg ccgcgtagag gccacagatg acatcgagca
     2281 gggcgacgcc caccttcgtg ggctcgccgc cgggctggcc ggtgatgctc atcaggccgg
     2341 atagggcctg cacgatgatg tcgtaaccgg gacgtgcggc gttctccccc tcctcggaga
     2401 acgcgagcaa ggagcagtag accagtcccg ggttcgcgcc cacgagcgcc tggtagtcca
     2461 gtccgaagga cctcatcagc ccggggcgga agttctcgat caccacgtcg gcccccagga
     2521 ccaggcgacg cgcgctcccg acccctcgat cggtggaaag atccagggtc accgatcgct
     2581 tgttccggtt gagggacagg aagtacgcgg cgtcatggcc cacgaagggc gggccccatc
     2641 cccgggtgtc gtcgcccaca tcgggccgtt cgaccttcac cacgtcggcc cccaggtcgg
     2701 ccaggatcat ggtggccaac gggccggcca gcacccgaga gaagtcgagc accctgatcc
     2761 cgctcagggg caggtccggt ccatcggagg cgagtcgctc gtcgagcacg gatcccctcg
     2821 ggtgcagagg tcacaaaagt ggaaccgccg gggcccgagg gctccagcgg ttccgagaga
     2881 tttatatcct ccccggatcc tcgccgtcaa atcgacgggc cgatcttgtc actctttctg
     2941 tggtgcccgc cgatcgcgct gccagccgag ggccccgatg ctggttcggc gagtggcgcc
     3001 gagcgccggg aattgccagg atatcggtgt gaacaaggag agcctggatc gggcgaacgc
     3061 attcctggcg gccgtggccg agcgggtcga gccgggcggg ttcctcgagg ccagtccggc
     3121 ggaggtcggt cgctcgatgg gcctgtcgga gccgctggcg gcggcgaggg cggtccgagc
     3181 cttgctggca cgtcggcggc tcgaggcggt ggatgggaga taccgcctgg tggatcctcg
     3241 gcccatcgaa ccgggggagc gggagcaggt gccgcggccc cgtcgtcccc gggcgccgaa
     3301 ggcgcgaacc ggcgaggagg ccccctcggg tcgacctacc tactccgagg tcggtcggga
     3361 ggccatcgag cggctgatcg agctcggtcg cgaggtggcc aacctgcgcg cgcacctgcg
     3421 gaccgctcgg gaggaggtgc gcgagtctcg ggaagcccgc gacgaggccg agcgccgggc
     3481 gcagacccta gcggttcggg tgcgcgagct ggagtcccgg gccgagatgg ccgagacgaa
     3541 cctccgcacc gtcctcgccg ccgccaaggg ggcggggaag gagctcccgg tgggcgacac
     3601 ggagatggag gccatcctgg gggtcctcaa gggtggcgcc gaggagcagg ccggggccca
     3661 gaccagctga ggtcaggcct tcgtcggctc ggtctcgccc tcgctcgctg ggggcgggct
     3721 cggcacccca cccctcgcca gccgggcggc caggtggcag gccacgaacc gcccctcgga
     3781 gagctgctcg agccccagat cctcctcccg gcacctcgac tccacgccgg ccgaggcggc
     3841 tcgcccctcc gcgagggcag ggcagcgcgg gtggaaccgg caccccggtg gcatcagcgt
     3901 gggatcgggc ggctctcctt gcaggaacgg acgatcgatg ccacccgcct cggggacgac
     3961 ctccagcagg gccttggtgt acggatgcat gggacgggtg aggacgtcct cggcgtcccc
     4021 gatctccacg atccgcccga ggtacatcac cgcgacccga tcggcgatgg tccacgccag
     4081 cccgaggtcg tgcgtcacca cgagcatcgc gatccccagg tcgttccgga gctgcaggag
     4141 caagcgcagg atctcgcccc gcaccgaggc atcgagactg gacaccggct cgtcggcgat
     4201 gatcacctca ggctcgagca ccaatgcccc cgcgatcacc acgcgctgac gctgaccccc
     4261 cgagagctcg tgcgggtagt tcaggaagaa gcgctccggc ggccgcaacc ccgctcggga
     4321 cagcgcccgg gccaccatgc cctcctccgc gaggccatcc tcgctcctat cgatgccgtg
     4381 gacacggagc ccctcggcca cggattcgta gatcgtctgg cgagggttca aggcggcggt
     4441 gggatcctgg aagaccatct ggacgcggcg tcggtactcg cgcaggttcc gggacaggag
     4501 cggcttgccc tcgaacagga tctctccgct ggtcggcttc tccagtccca tgatcgtccg
     4561 ggccagggtg gtcttcccgc agcccgactc gccggccagg gccacgacct ccccggggta
     4621 gagctcgaga tcgacaccgt cgaccgccct ggccacggtg ggcttgcgtc ccagcaggcc
     4681 cgagcccaga cccatctgtc ccgcgaagtg cacgtggacc tcccgggcct cgagcaccgg
     4741 cagggcgccc tcggaccgcg ctcggaccgc ctccgcggcg atctcgctca cggctccgcc
     4801 tccgccatct ccgacctggg gacgagcagg caggcggcgc gacgggcctc cccggcctgg
     4861 aacagctcag ggtcgatgtg cgggcagtcc tcgaaggcgc gggggcatcg aggatggaac
     4921 gagcatccgg agggaacgtc cccagggaag ggcgggtcgc ccgggaggcc ggagggggcc
     4981 atccggtagg tgcgatcccc gatctgcggg aaggctccgg ccagagccct cgtgtaggga
     5041 tgcgccggat cggtgaagac ggggatcgac gggccctcct ccacgatctt gccggcgtac
     5101 atgatggcca gcttgtcgct cacctcgacc aggacagaca ggtcgtgggt gatgaacagc
     5161 atggccaggc cgaggtcccg ttgcaggtcc ttgaggagtc ggagcacctg cgcttgcacc
     5221 atgacgtcga gggccgtggt gggttcgtcc gcgatcacga tcttcgggtt gcaggccagc
     5281 gccatggcga tcatcacccg ctgcttctgc cctccggaca gctcgtgggg gaagtcccgg
     5341 agccgccgag ggggcagccc cacctgctcg agcagggagg cggcgcgcgt catggcctcg
     5401 gattcaccgg cctgcccgtg gaccagcgcc gcctcggcga tctgcgcacc gatccggcgg
     5461 acggggttca gcgcgtgcat ggcgccctgg aagaccaccg aggcctgggt ccagcggacc
     5521 gcgcggagcc gccccggtcg catggccagg acgtcctcgc cgtccaggag cacggtgccc
     5581 tccacccggg tctggggcgg caggagccgg aggatcgcgt tgatcacggt ggtcttgccg
     5641 catcctgact cgccggccag acccaggacc tcgccccgac cgatttccag gttcacgccc
     5701 cgaaccgccg gcacaggccc cgcctgggtg tggtaggtga tctgcaagtc tcggaccgag
     5761 agcagcgtca tcgttccctc agcttcgggt tgaggatctc atccagcgcg aagccacaca
     5821 tcgtgaaggc cagcaccacc aagacgatgc acacgcccgg cggaagcagc caccaccagt
     5881 tcccggctcc cgccgctccc gagttgaagg cgaactcgag gatcgttccc caggagatgc
     5941 tgatggggtc gcccaggccg aggaaggaca gcgtcgtctc ggacaagatc gcgatcgcca
     6001 cggtcaggat cgtgttggcg aagatcaggg gcccgacgtt gggaaggatg tgccgcgtga
     6061 tgaggtgcca gttgctggcg cccagcgctc gagcccgttc cacgtacggg cgggtcttga
     6121 ccgagagggt ctgtgcccgc accaggcgag cggtgctggg ccacgaggtg atgccgatca
     6181 cgaagatgat cacgaccagc gacggaccga tgagcgcggc cagcacgatg gccagcgcca
     6241 gccagggaag caccaggaac cagtccgtca cccgcatcag cacggtctcg ggccagccgc
     6301 cgtagtagcc ggcggcgata ccgatcagcg ccccgatccc catcgagatc agggtcgcgg
     6361 tcaggccgac gatcagcgag atccgcgagc cccacacggt gagcgcgaac accgaccgtc
     6421 ccaggtcgtc ggtcccgaac gggaacttca gactgggggc ctggaacggc gcaccggtgc
     6481 acgtgcaggt cgggtcgagt tgggcatggc tggcgatcaa cggagcgagg acggcgacca
     6541 gcacgaagaa ggcaaggatg tagaggcccg ccatccccat ccggttgccg cggtagatcc
     6601 tccacacacg ggacagggcg cggcggcgac ggatccgggc gatcttgcgc gggtcggtcg
     6661 gcggggggag cccgacggtc tcctcgccga ccgggggcgg tggcggctcg gtgacctgtc
     6721 cgatctcggg ccgcatgtca ggccgccctc acgcgcgggt cgaggtacgt gtaggtgatg
     6781 tccgcgatca ggttggcgaa gatgaccgca ccgctgaaca ggaggaagag tgcctggagc
     6841 agcgggaagt ccttcg
//
LOCUS       DASQQQ010000331         5408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_134984, whole genome shotgun sequence.
ACCESSION   DASQQQ010000331 DASQQQ010000000
VERSION     DASQQQ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134984"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VE646_12425"
     CDS             <1..520
                     /locus_tag="VE646_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019886158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZD80828.1"
                     /translation="EVASIAERRAAPAISAEAARCRGAVRLAGGDPAAALHDLRRAWT
                     TWQEIGLPYQSARARMLYGTALRATGDEDGARLELRAALSTFERLGAVPDAAATTALL
                     RDGAELPGGISAREAEVLRLVAAGKTNREIAEALVISPHTVARHLQNIFAKLDVASRS
                     AATAFAVEHGLA"
     gene            704..1813
                     /locus_tag="VE646_12430"
     CDS             704..1813
                     /locus_tag="VE646_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009477554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZD80829.1"
                     /translation="MKAPERFETVIVGGGQAGLAIGYHLMKRGRPGVILDASERIGDA
                     WRTRWDSLRLFTPAKYDGLPGWRFPGPGWSFPTKDEMADYLEAYASRFALDVRTGVRV
                     DRLSRQGHRFVLTAGERRFEADDVVIASGAHHIPKVPPFAFDLDRGIVQMHSKHYRNP
                     SQLREGPVLVVGLGNSGAEIAFELSRRLRTIVAGEPSAQIPFRHGGFAGRVAIPGVRF
                     LGHHVLTRRTPIGRRLAAKFEHGAAPLIRVRVKELLAAGVERVPRVIGVRDGLPLLQD
                     GRVLEVSNVIWCTGFRQDLSWIDLPSFGQTGPPPHVRGVVPSEAGLYFMGLRFQFSES
                     SDTLVGLDRDGAYVAKAIALRQERSLLPEDALVTA"
     gene            complement(1973..2050)
                     /locus_tag="VE646_12435"
     tRNA            complement(1973..2050)
                     /locus_tag="VE646_12435"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2013..2015),aa:Lys,seq:ttt)
     gene            2153..2887
                     /locus_tag="VE646_12440"
     CDS             2153..2887
                     /locus_tag="VE646_12440"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HZD80830.1"
                     /translation="MNRRGALVAGVAVVALLVGFGARAMAGNGDPSSQKPVHTITVTS
                     TATVKAAPDEAVVDLGVSTSSPDSARAFAQNAADMQAVLDALKAAGIQQKDLQTLNVS
                     LDQRTVDKGKPGEHREFVATNRVQVTVHDLSAVGSIIDAAVQAGADSVGSIDFRLANP
                     NTVRADALASAVRGARAKADALAAAADAQVVRVVTIQEQNFRTPMYSEALPAGFGAGA
                     VRAVTPIVPPDTLQASVTVSVVWEIS"
     gene            complement(2895..4577)
                     /locus_tag="VE646_12445"
     CDS             complement(2895..4577)
                     /locus_tag="VE646_12445"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HZD80831.1"
                     /translation="MRIALGQLNCTVGDLGGNVERMASWTGEATEAGADLICFPELAV
                     TGYPPEDLVLRPEFVRDNLEALDDLARRTEGGCAVLTGFVDRSERGLHNAAALLSGGA
                     VTARYHKVKLPNYGVFDERRYFVPGDQACPVRVASSELGISVCEDAWTPGAPFDTYAD
                     LGVRVIPNINGSPYHRHKTWERLEICRDRARETGAWIVYVNLVGGQDELVFDGGSMVV
                     SPEGELAWHAAAFEEDLLIVDLEVSTEDPAPAPANRAPALSDPSRPPWPEGPEEVYRA
                     LVLGLGDYARKNGFSQAVLGLSGGIDSALTATVAADALGSAAVRALAMPSPYSSPESV
                     EDAQELARRLGITFDRMGIDEVFRAYLSTLEEAFRGTEPGVAEENVQARIRGNLVMAI
                     SNKFGALALATGNKSEYAVGYSTLYGDMAGGFAPIKDLPKTLVYDLAAWRNAQEATPP
                     IPDRVMTKPPSAELRPDQLDTDSLPPYGELDPIVEAYVEEDRSAEEIVAAGFDPSLVE
                     RVAGMIDRAEYKRRQSPPGVKITPKAFGRDRRLPITNRYRTAPPGGRPHRTR"
     gene            complement(4640..5119)
                     /locus_tag="VE646_12450"
     CDS             complement(4640..5119)
                     /locus_tag="VE646_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80832.1"
                     /translation="MERNQGNDPRSSAAFEEAKLRRADLHASLIALEEAISSPASGRL
                     EQWTADVTKRLGQLLQTIDEHIEVTERPGGLYEEIRMRAPRLSGSIDRLHDEHPLVRE
                     TTRDLVRKFESTPIGEDWELRDARDEVQRLLGKLVRHRQDGADLVWEAYNLDIGGHE"
     gene            complement(5160..>5408)
                     /locus_tag="VE646_12455"
     CDS             complement(5160..>5408)
                     /locus_tag="VE646_12455"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZD80833.1"
                     /translation="EAETLAREAVRLVRTSDEPDSQASALMDLGETLVLVGKLREAVP
                     TFEEAIALLEAKGNVVSATRARTRLANLEVAAPGPARL"
BASE COUNT          765 a         1866 c         1946 g          831 t
ORIGIN      
        1 ggaggtggcg tcgatcgccg agcgacgagc ggccccagcg atctcggcgg aggcggccag
       61 gtgccgcgga gccgtgcgcc tggccggggg cgatcccgcg gccgccctcc atgacctccg
      121 ccgcgcctgg accacatggc aggagatcgg actgccctac cagtcggccc gggctcggat
      181 gctgtacgga accgcgctcc gggcaacggg agacgaggac ggagcgcgac tggagctgcg
      241 ggcggcactg tcgaccttcg aacggctggg ggcggtgccc gacgcggccg cgacgaccgc
      301 cctgctccgc gacggagcag agctccccgg gggcatctcg gctcgggagg cggaggtcct
      361 gcggctcgtg gcggccggca agaccaaccg cgagatcgcc gaggcgctgg tcatcagccc
      421 gcacacggtg gcccgacacc tccagaacat cttcgccaag ctcgacgtgg catcacgctc
      481 cgccgccacg gcgttcgccg tggagcacgg cctggcctga actcccgtct ccgggccgcc
      541 cacgccgacc ccccatgccc ctgcgtctgg tcggtcccag cagtcgatcg atcgatggtc
      601 aaccttgacc atccccgcca accgtccagg ttggctggtt cggtcgatga gcccggcccg
      661 gcgtccgtcc taccgtctgg ccatggcagg aaggaggctc ctgatgaagg ctccagaacg
      721 gttcgagacg gtgatcgtgg gcggcggcca agcggggctc gcgatcggct accacctgat
      781 gaagcggggg cggccgggcg tgatcctcga cgcgtcggaa cggatcggcg acgcgtggcg
      841 gacccgctgg gactcgctgc gcctgttcac gccggccaag tacgacgggc tgcccggctg
      901 gaggttcccc gggcccggct ggtcgttccc caccaaggac gagatggccg actacctcga
      961 agcgtacgcg agccggttcg cgttggacgt ccgcacgggt gtacgggtcg accgcctctc
     1021 gaggcagggc catcgcttcg ttctcaccgc aggcgagcgc cggttcgagg cggatgacgt
     1081 ggtgatcgcc tcgggcgccc accacatccc gaaggtgccg ccgttcgcgt tcgatctgga
     1141 tcgagggatc gtgcagatgc actcgaagca ctaccgcaac ccttcacagc tccgggaggg
     1201 ccccgtcctg gtcgtggggc tggggaactc cggtgccgag atcgcgttcg agctgtcccg
     1261 gaggcttcgg acgatcgtcg cgggggagcc gagcgcgcag atcccgttcc ggcacggtgg
     1321 tttcgccggc cgggtggcga tcccgggggt ccggttcctg gggcatcacg tgctgaccag
     1381 gcgaacgccc atcggccgga ggctggcagc gaagttcgag cacggcgccg cgccgctgat
     1441 ccgggtgcgc gtgaaggagc tgttggcggc gggggtggag cgcgtgccgc gggtcatcgg
     1501 cgttcgcgac gggctcccgc tgctccagga tggtcgtgtc ctcgaggttt ccaacgtgat
     1561 ttggtgtacg ggtttccgcc aggacttgtc ttggatcgat ctgccctcct tcggccagac
     1621 cgggccgccg ccgcatgtcc gaggggtggt cccgtccgag gcgggtctgt acttcatggg
     1681 tctacggttc cagttctcgg agtcctcaga cacgctggtt gggctggatc gggacggggc
     1741 gtacgtcgcc aaggcgatcg cgttgcggca ggagcgctcc ctgctgcccg aggacgccct
     1801 ggtcaccgcg tgagtcctct aagccagcat gcgtgcaaga tgtgcgtccc ggaccggctc
     1861 cgagcgcggc gccagggcgg catggtggcc gccaccaggg gcatgcacca ccttcgggac
     1921 gggggatccg gccatggagc ggtgacacaa cgggctctac cagcggctct cctggtgggc
     1981 cgggcaggat tcgaacccgc gacacgaggg ttaaaagccc cctgctctga ccagactgag
     2041 ctaccggccc cgccgagatt ctagggaacg gaacccctct cgccggtgac gcgtcccact
     2101 tgcgatgacg ccgtccgagg accgggcggc gcacggcgga tgggaggtac gcatgaacag
     2161 gcgaggcgcg ctggtcgccg gcgtggcggt ggtggcgttg ctggtggggt tcggagctcg
     2221 ggccatggcg gggaacggcg acccctcctc ccaaaagccg gtgcacacga tcacggtcac
     2281 ctccaccgcc acggtgaagg cggctcccga cgaggccgtg gtggacctgg gcgttagcac
     2341 ctcatcgccg gacagcgccc gagcgttcgc gcagaacgcg gcagacatgc aggcggtgct
     2401 cgacgcgctg aaggccgccg ggatccagca gaaggacctg cagaccctga acgtctcgct
     2461 ggatcagcgg acggtggaca aggggaagcc gggagagcat cgggagttcg tggccacgaa
     2521 ccgggtgcag gtcacggtgc acgatctctc ggcggtgggt tcgatcatcg acgcagcggt
     2581 gcaggcgggt gccgactcgg tcggcagcat cgacttccga ctggcgaacc cgaacacggt
     2641 tagggccgac gctctcgcct cggcggtgcg gggggcccgg gccaaggcgg acgcgctggc
     2701 cgcggccgcc gacgcccagg tggtccgggt ggtcacgatc caggagcaga acttccggac
     2761 gcccatgtac tcggaggccc tgccggccgg gttcggagcg ggagcggtga gggccgtgac
     2821 ccccatcgtt ccacccgaca cgctgcaggc cagcgtgacc gtgagcgtgg tgtgggagat
     2881 ctcctgatcg cggatcaccg agttcggtga ggccgtcctc ccggaggagc ggtgcggtag
     2941 cggttggtga tgggcaggcg ccggtcgcgg ccgaaggcct tgggcgtgat cttcacgccc
     3001 ggcggcgact ggcgccgctt gtactcggcc cgatcgatca tcccggccac ccgttccacc
     3061 agcgatgggt cgaagcccgc cgccacgatc tcctcggcgg accggtcctc ctccacgtac
     3121 gcctcgacga tgggatcgag ctccccgtac ggcggaaggc tgtcggtgtc cagctggtcg
     3181 ggacggagct cggccgacgg gggcttggtc atgacgcggt ctgggatcgg cggggtggcc
     3241 tcctgggcgt tccgccaggc cgcgaggtcg tacacgaggg tcttgggcag gtccttgatg
     3301 ggcgcgaagc cgcccgccat gtccccgtac agcgtcgagt agcccaccgc atactcgctc
     3361 ttgttaccgg tggccagcgc cagcgctccg aacttgttcg agatggccat caccaggttg
     3421 ccgcggatcc gggcctggac gttctcctcg gccacgccgg gctcggtgcc tcggaacgcc
     3481 tcctccaggg tcgacaggta ggcgcggaac acctcgtcga tccccatccg gtcgaaggtg
     3541 atccccaggc gccgagccag ctcctgcgcg tcctccacgc tctcgggaga tgagtacggg
     3601 gagggcatgg ccagcgcccg gaccgcggcg gaaccgagcg cgtctgccgc aaccgtggcc
     3661 gtgagggccg agtcgatccc tcccgacagg cccaggaccg cctgtgagaa gccgttcttg
     3721 cgcgcgtagt cgccgagccc gagcaccagc gcccgataga cctcctccgg cccctccggc
     3781 cacggcggcc gggaggggtc ggacagcgcc ggagcccgat tggcaggggc cggcgccggg
     3841 tcctcggtgg agacctccag gtccacgatc agcagatcct cttcgaacgc cgcggcgtgc
     3901 caggcgagct ctccctcggg ggacacgacc atgctgccgc cgtcgaacac cagctcgtcc
     3961 tgccccccga ccaggttgac gtacacgatc catgctccgg tctcgcgggc gcggtcccgg
     4021 cagatctcca ggcgctccca ggtcttgtgc cggtggtatg gggagccgtt gatgttcggg
     4081 atcaccctca cgccgaggtc cgcgtaggta tcgaaaggag cgccgggcgt ccaggcatcc
     4141 tcgcagacgg agatccccag ctccgacgag gccacccgga cggggcacgc ctggtctccg
     4201 ggcacgaagt agcgtcgttc gtcgaacacg ccgtagttcg gcagcttcac cttgtggtac
     4261 ctggcggtga cggcgccccc ggacagcagc gccgcggcgt tgtgcagccc gcgctccgac
     4321 cggtccacga atccggtgag gacggcgcac ccaccctcgg tgcgtctggc caggtcgtcc
     4381 agggcctcga ggttgtcgcg gacgaactcg gggcgcagga ccaggtcctc gggcgggtac
     4441 ccggtgaccg cgagctcggg gaaacagatc aggtccgcgc cggcctcggt ggcctcgccg
     4501 gtccaggagg ccatgcgctc cacgttgccg ccgaggtcgc ccacggtgca gttcagctgt
     4561 ccgagcgcga tacgcaccac gggccacagc gtagccgttc ggtcgtgttg ccccgccgcg
     4621 gagcttcggc ggctcggtgc tactcgtgcc cgccgatgtc caggttgtag gcctcccaga
     4681 ccaggtcggc cccgtcctgc cggtggcgca ccagcttgcc cagcaggcgc tgcacctcgt
     4741 ccctggcatc cctgagctcc cagtcctccc cgatcggcgt ggactcgaac ttgcggacca
     4801 ggtctcgcgt cgtctcccgg accagcgggt gctcgtcatg gagacgatcg atcgagccgg
     4861 acaggcgcgg ggcccgcatg cggatctcct cgtacagccc tccgggccgc tcggtgacct
     4921 cgatgtgttc gtcgatcgtc tggagcagct ggcccagccg cttggtgacg tcggccgtcc
     4981 actgctcgag ccggccggag gccgggctgg agatcgcctc ctcgagggcg atcagagagg
     5041 cgtgcagatc ggctcgccgg agcttggctt cctcgaacgc ggcgctcgat cgcgggtcgt
     5101 tcccctggtt ccgctccatg gagacctcct cacccgcggg ctgctggccc ggggcgcaac
     5161 tataggcggg ctgggcctgg cgccgccacc tcgaggttgg ccaggcgggt ccgcgcccgg
     5221 gtggccgaga ccacgttgcc cttggcctcg aggagcgcga ttgcttcctc gaacgtgggg
     5281 acggcctccc tcagcttgcc gaccaggacc agcgtctccc ccagatccat cagggcgctg
     5341 gcctgggaat cgggctcatc ggaggtccgg accagccgga ccgcctccct ggcgagggtc
     5401 tcggcctc
//
LOCUS       DASQQQ010000332         6042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_270459, whole genome shotgun sequence.
ACCESSION   DASQQQ010000332 DASQQQ010000000
VERSION     DASQQQ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270459"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /gene="hpnE"
                     /locus_tag="VE646_12460"
     CDS             <1..992
                     /gene="hpnE"
                     /locus_tag="VE646_12460"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HZD80834.1"
                     /translation="GHLPPVDRLRAMRAAAALARSPRLEQADDRTFQDWLQERGQRPT
                     AVRTLWNLIALPALNLPADQAALGPAAKVFRTGLLLDRAAGDVGYAVAPLARVHGEPA
                     ARALEAAGAEVRVKTAVRSIELQSGLLRVHADGTAWDSEAVILAAPHEAAADLLPDGA
                     IPGVSRLSELGSSPIVNLHVVYDRPVMDLPFAAAVDSPVQWVFDRSKTSGLVEGQYLA
                     VSLSGAAAEIDEPTGRLRERYLPALSALFPRVRAARILDFFVTRERRATFRAAPGSAA
                     LRPPTRTDVPGLFLAGAWTATGWPATMEGAVRSGEAAATAARDALRNRPVVAA"
     gene            989..2002
                     /locus_tag="VE646_12465"
     CDS             989..2002
                     /locus_tag="VE646_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZD80835.1"
                     /translation="MIGTVPEALPRAREMVEPALRACVDRLSPEIRRVVGYHLGLADA
                     DGTPTGSLTGKAVRPALAFLSAEAAGAPAEVGLPGAVAVELIHNFSLLHDDVMDRDRE
                     RRHRPTAWTLFGVGAAITAGDALQTQAHELLLDPPTPARVRAASALASATAKMIVGQA
                     QDLSFESRLDVSPEESRRMCANKTGALLSCAASIGAILAEAPAPTVRALSEFGLHLGL
                     AYQAIDDILGIWGRPEITGKPVANDLREHKKSLPVAEALSFGGPASDRLHELLSDGSV
                     NDGALQEAMGLLEGIGARPMAEREADREIEAALGALDREGIQERVRERLSEIARFVTA
                     RQA"
     gene            2073..3953
                     /gene="shc"
                     /locus_tag="VE646_12470"
     CDS             2073..3953
                     /gene="shc"
                     /locus_tag="VE646_12470"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018384768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HZD80836.1"
                     /translation="MTQTTDGTEARAAAALEHAAARLLELQEPAGWWKGELQTNVTMD
                     AEDLLLRAFLGLEDERITLPAAEWIRSQQREDGTWSNFHGGTPDLSTTIESYWALKLS
                     GDPIEAAHMRRAASWILEAGGLERARVFTHLWMALFGLWRWEDVPALPPEMIFLPAWF
                     PLNIYYFACWARQTVVALTVVGAHRPVRPLPFDLSELRTGAPAPPMESLRTWAGRFQR
                     LDRVLQAYERRPIRRLRRLALARAIQWIVRRQEADGCWGGIQPPWVYSIIALHVSGFP
                     LDHPVIRSALAGIERFTIHEDGVRRLEACQSPVWDTALAMTALADAGTEPDHPALLRA
                     TDWMLDEEIRVPGDWIIRRPHLEPGGWAFEFDNDNYPDIDDTAEVILALRRVRHPHPH
                     RVCEAIDRGVRWVLGMQSSDGAWGAFDADNTRALCRELPFCDFGEVIDPPSADVTAHV
                     LEMLAAEGRAQDPAALRGLTWLASEQEPGGSWFGRWGANHVYGTGAAVPAAIACGVPS
                     DDPRILSAVRWLEEHQNEDGGWGEDLRSYREPSWVGRGASTASQTAWALLALLAAGKR
                     GDVVRRGIRWLCDTQRPDGGWEEPAFTGTGFPGDFYINYHLYRLVFPVSALGRFVRGD
                     AR"
     gene            3950..5524
                     /locus_tag="VE646_12475"
     CDS             3950..5524
                     /locus_tag="VE646_12475"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HZD80837.1"
                     /translation="MSSAARDRDRTSSRRSDVAPRTGDLLVFAPLAIEARAVRRSVTR
                     GTVLRTGGGPERAARQVGAVEAAAPRAVAVTGFGGGLDPSLSPGDIVVATEVRGPSGV
                     RPCPGAELFAAELRRRGLPARTGPVISVAHLVTGKDRRRLAERGALAVDMESAWLAEA
                     VGDRPFLVVRAIVDTVGSELLSPSTVSGGLRAARTLRRAAAALDAWVDALGAHQVLLA
                     APRSFCAGVDRAIEIVERALDRHGPPVYVRKQIVHNVHVVQDLERRGAVFVDSATEVP
                     EGSLIVFSAHGVSPEVRRQADERRLRVIDATCPLVTKVHAEARRFARAGYTIFLIGHE
                     GHEEVEGTTGEAPDAIRLVDGDASDDLNVADPSRVAYLTQTTLAVDDVNEVLTGLRER
                     FPQMSGPGSDDICYATQNRQDAVKALAHACDLLLVVGSENSSNSNRLVEVSAKEGTPA
                     YLVDDENDVELSWLKGVATVGITAGASAPERLVDRVIASLRGLGTLEVEERQVVDESM
                     RFTLPVELRDVNERTR"
     gene            5532..>6042
                     /gene="hpnH"
                     /locus_tag="VE646_12480"
     CDS             5532..>6042
                     /gene="hpnH"
                     /locus_tag="VE646_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016878349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HZD80838.1"
                     /translation="MSVPIRQSVRMAAYLAKQRLRKKERFPLIVELEPLYACNLACAG
                     CGKIQYPDHILDKRLAPEQCWAAAEECGAPIVSIPGGEPLIHPEMPAIVRGLVERKKY
                     VYLCTNAILLERKLADYEPSKYLTFSIHMDGLREEHDHAVCRDGVYDVAVRAIRAALA
                     RGFRVTTNTT"
BASE COUNT          821 a         2123 c         2238 g          860 t
ORIGIN      
        1 acggtcacct cccgccggtg gaccgcctcc gcgcgatgcg ggccgccgct gccctggccc
       61 gctcgccgag gctggagcag gccgacgacc ggacgttcca ggattggctc caggagcggg
      121 ggcagcggcc aaccgccgtc cggaccctgt ggaacctgat cgccctgccg gccctgaacc
      181 tcccggcgga ccaggcggcg ctcggtccgg ccgccaaggt cttccggacc ggcctgctgc
      241 tcgaccgggc ggcgggagac gtgggctacg ccgtcgcccc gctcgcgcgc gtccacgggg
      301 aacccgctgc cagggccctc gaggcggccg gggccgaggt ccgcgtgaag acggccgtac
      361 gctccatcga gctccaaagc ggcctgctgc gcgtgcacgc tgatggcacg gcctgggact
      421 ccgaggccgt gatcctggcc gcaccgcacg aggcggcggc cgatctcctg ccggacgggg
      481 cgatccccgg tgtgtcccgc ctgagcgagc tcggctcctc gcccatcgtg aacctgcacg
      541 tggtctacga ccgtccggtg atggacctcc ccttcgcggc ggccgtcgac agccccgtgc
      601 agtgggtgtt cgatcgctcg aagacctccg gcctggtgga aggtcaatac ctggccgtct
      661 ccctgtcggg cgcggcggcc gagatcgacg agcccacggg ccggctccgg gaacggtacc
      721 tgccggcgct gtcggcgctg ttcccgcggg tccgggcggc gcggatcctg gacttcttcg
      781 tgacccggga acgcagggcg accttccggg cggctcccgg gtcggcggcc ctgcgtccgc
      841 cgacccgaac cgacgtcccc ggcctgttcc tggctggcgc ctggaccgcg accggctggc
      901 cggccacgat ggagggcgcg gtgcggagcg gcgaggccgc ggccaccgcg gcccgggacg
      961 ctctgcggaa ccgaccggtg gtggcggcat gatcgggacc gtccccgagg ccctgccccg
     1021 cgcgcgagag atggtcgagc cggcgctccg ggcatgcgtg gaccgcctct cgcccgagat
     1081 ccggagggtg gttggctacc acctggggct ggccgacgcc gacgggaccc cgaccgggtc
     1141 cctcacaggc aaggccgtgc gtcccgctct ggcgttcctg tcggcggagg cggccggggc
     1201 cccggccgag gtgggcctgc ccggggcggt ggcagtggag ctgatccaca acttctcgct
     1261 gctacacgac gacgtgatgg accgggaccg cgagcggcgg caccggccca cggcctggac
     1321 cctgttcggc gtgggcgccg ccatcaccgc cggcgacgcg ctccagaccc aggcccacga
     1381 gctcctgctg gacccgccca ccccggctcg ggtccgggcg gcgtccgccc tggcctcggc
     1441 caccgcgaag atgatcgtgg gccaggcgca ggatctctcg ttcgagtcgc ggctggacgt
     1501 ttcccccgag gagagccgcc gcatgtgcgc gaacaagacc ggcgctctgc tgtcgtgcgc
     1561 cgcctccatc ggcgccatcc tggcggaggc gccagccccg acggtgcggg cgctctcgga
     1621 gttcggtttg catctcgggc tggcctacca ggcgatcgac gacatcctgg ggatctgggg
     1681 ccggcccgag atcaccggaa agcccgtcgc caacgatctt cgtgagcaca agaagagcct
     1741 gcccgtggcc gaggcgctgt cgttcggggg gccggccagc gaccggctgc acgagctcct
     1801 gtccgacggc agcgtgaacg acggtgccct acaggaggcg atgggtctgc tcgaggggat
     1861 cggcgcgcgc cccatggccg aacgcgaggc ggatcgcgag atcgaggccg ccctgggggc
     1921 gcttgatcgt gagggcatcc aggaacgggt acgggagagg ttgagcgaga tcgcgcgctt
     1981 cgtgacggcg aggcaggcct gatgaccccc gccgctcgcg gagcgaagac cgggaaggcg
     2041 aagaccggga aggacggaac gcccgcgtgg tgatgaccca gacgactgac ggaaccgagg
     2101 cccgggccgc ggcggcgctg gagcacgccg ccgccaggct gctcgagctg caggaacccg
     2161 ccggctggtg gaagggcgaa ctgcagacca atgtgacgat ggacgccgag gatctgctgc
     2221 tgcgggcgtt cctggggctc gaggacgagc ggatcaccct ccccgcggca gagtggatca
     2281 ggtcgcagca gcgtgaggac ggcacctggt ccaacttcca cggtggcacg ccggacctgt
     2341 ccaccacgat cgagtcgtac tgggccctga agctgtccgg ggatccgatc gaggccgcgc
     2401 acatgcgccg cgcggcgtcc tggatcttgg aggccggggg gctggagcga gcgcgcgtct
     2461 tcacccacct gtggatggcg ctgttcggcc tgtggcgctg ggaggatgtt ccggcgctgc
     2521 ccccggagat gatcttcctg cccgcctggt tccccctgaa catctactac ttcgcctgct
     2581 gggccaggca gacggtggtg gcgttgacgg tggtgggagc gcaccgcccg gtccggcccc
     2641 tcccgttcga cctgtcggag ctccggaccg gtgcgcccgc gccgcccatg gagtcgctcc
     2701 gcacctgggc cggccggttc cagcggctgg accgggtcct gcaggcgtac gagcgtcggc
     2761 cgatccggcg cctccggcgc ctggcgctgg cccgcgcgat ccagtggatc gtccggcgcc
     2821 aggaggccga tggctgctgg ggcgggatcc agccgccatg ggtgtactcg atcatcgcgc
     2881 tgcatgtctc cggcttcccc ctggaccatc ccgtgatccg ctcggcgctg gccgggatcg
     2941 agcgcttcac gatccacgag gacggcgtcc gccgcctgga ggcctgccaa tcaccggtgt
     3001 gggacacggc gctggccatg acggcgctgg ccgacgcggg gaccgaaccc gaccacccgg
     3061 cgctcctccg ggcgaccgac tggatgctgg acgaggagat ccgtgtcccc ggggactgga
     3121 tcatccgccg tccgcatctg gagccgggcg ggtgggcctt cgagttcgac aacgacaact
     3181 accccgacat cgacgacacg gccgaggtca tcctggcgct ccggcgcgtg cggcatcccc
     3241 atccccaccg ggtatgcgag gcgatcgacc gcggggtgcg atgggtcctc ggcatgcagt
     3301 cctcggacgg ggcgtggggt gccttcgacg ccgacaacac cagggcgctc tgccgggagc
     3361 tgccgttctg tgacttcggc gaggtgatcg acccgcccag cgccgacgtg acggcccacg
     3421 tcctggagat gctggccgcc gagggtcggg cccaggaccc ggccgcgttg cgcgggctga
     3481 cctggttggc ttccgagcag gagcccgggg gctcgtggtt cgggcgctgg ggcgccaacc
     3541 acgtgtacgg caccggcgcg gcggtcccgg cggccatcgc ctgtggcgtc ccctccgacg
     3601 acccgcggat cctatcggcc gtgcgctggc tggaggagca ccagaacgag gacggcggct
     3661 ggggcgaaga tcttcgctcc taccgagagc cctcgtgggt cggtcgcgga gcgagcaccg
     3721 cgtcccagac cgcctgggcc ctgctggcgc tcctcgcggc ggggaaacgc ggcgacgtcg
     3781 tccgacgtgg gatccgctgg ctgtgcgaca cccaacgacc ggacggaggc tgggaggagc
     3841 ccgccttcac ggggaccggc ttccccggtg acttctacat caactaccac ctgtaccggc
     3901 tcgtcttccc ggtgtcggcg ctcggcaggt tcgtgagggg tgatgctcgg tgagctcggc
     3961 tgcccgagat cgggatcgga cctcctcccg ccgatccgac gtcgcaccga ggaccggcga
     4021 tctgttggtc ttcgcgcccc tcgccatcga ggctcgggcc gtgcgccggt cggtcacccg
     4081 gggcacggtg ctccgaacgg gcggaggacc ggaacgagcg gcgcgccagg tcggagcggt
     4141 cgaggcggcc gccccgcgcg ccgtggcggt gaccgggttc ggcggcgggc tggatccatc
     4201 gctgtccccc ggcgacatcg tggtggccac cgaggtgcgc ggaccctccg gcgtccggcc
     4261 ctgccccgga gcggagctct tcgcggccga gctgcggcgc cgagggctcc ccgctcgcac
     4321 cgggccggtg atctcggtgg cgcatctcgt gacgggcaag gaccgccgac gactcgcgga
     4381 gaggggggcc ctggccgtgg acatggaatc cgcgtggctg gccgaggcgg tgggcgaccg
     4441 tcctttcctg gtggtgcggg cgatcgtgga caccgtcggc tcggagctgc tgtcgccctc
     4501 gaccgtgagc ggcggcctgc gcgccgcgcg gaccctgcgc cgggccgcgg ccgccctcga
     4561 tgcgtgggtg gacgcgctgg gcgcccatca ggtcctgctg gctgcccccc gctcgttctg
     4621 cgccggggtg gatcgagcca tcgagatcgt ggagcgcgcc ctggatcggc acggaccgcc
     4681 cgtgtacgtg cgcaagcaga tcgtccacaa cgtccacgtg gtgcaggatc tggagcgacg
     4741 gggcgcggtg ttcgtggact cggccaccga ggtccccgaa gggtccttga tcgtcttctc
     4801 cgcccacggg gtctcgccgg aggtgcggcg ccaggccgac gagcggcggc tccgcgtgat
     4861 cgacgccacc tgcccgctcg tgaccaaggt ccacgccgag gcccgccggt tcgcgcgcgc
     4921 cgggtacacg atcttcctga tcgggcatga gggccatgaa gaggtcgagg ggaccaccgg
     4981 cgaggcaccg gacgcgatcc ggttggtgga cggcgacgcc agcgacgatc tgaacgtcgc
     5041 cgatccctcc cgtgtggcct acctcacgca gaccacgctg gcggtggacg acgtgaacga
     5101 ggtgttgacc ggcctgcggg agcggttccc gcagatgtcc ggccccggct ccgacgatat
     5161 ctgctacgcc acgcagaacc gccaggacgc ggtgaaggcg ctggcgcacg cgtgcgacct
     5221 cctgctggtg gtcgggtccg agaactcctc gaactcgaac cgtctggtgg aggtttcggc
     5281 gaaggagggg acaccggcct acctggtgga cgacgagaac gacgtggagc tgtcgtggct
     5341 gaagggcgtg gccaccgtgg ggatcaccgc gggcgcctcg gcccccgagc gcctggtgga
     5401 ccgggtgatc gcgtcgctcc gcgggctggg caccctggag gtggaggaac ggcaggtggt
     5461 ggacgagtcc atgcggttca cgctgcccgt cgagctccgc gacgtgaacg agagaacgag
     5521 gtgatatccg catgagcgtc ccgatcagac agagcgtccg gatggccgcc tacctagcca
     5581 aacagcggct ccgcaagaag gaacggttcc ccctcatcgt ggagctcgag cccctgtacg
     5641 cctgcaacct ggcctgcgcc gggtgcggca agatccagta ccccgaccac attctcgaca
     5701 agcggctcgc ccccgagcag tgctgggcgg ccgcggaaga atgcggggct cccatcgtca
     5761 gcatcccggg cggggagccg ttgatccatc cggagatgcc ggcgatcgtc cgcggcctgg
     5821 tcgagcggaa gaagtacgtg tacctgtgca ccaacgccat cctcctcgag cggaagctgg
     5881 ccgactacga gccgtcgaag tacctcacgt tcagcatcca catggacggg ctgcgtgagg
     5941 agcacgatca cgccgtgtgc cgcgacggcg tctatgacgt ggccgtgcgg gcgattcgcg
     6001 ccgccctggc gcggggcttc cgcgtgacga cgaacaccac cc
//
LOCUS       DASQQQ010000333         6720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_135099, whole genome shotgun sequence.
ACCESSION   DASQQQ010000333 DASQQQ010000000
VERSION     DASQQQ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135099"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VE646_12485"
     CDS             complement(<1..246)
                     /locus_tag="VE646_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005246368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZD80839.1"
                     /translation="MGAQGRESADLVFTGGAVYTVDAARRWAEAAAVRSGKVVAVGTD
                     EAVREVIGPRTEVVELEGRMLLPGFQDAHAHPPAGGLE"
     gene            complement(301..1896)
                     /locus_tag="VE646_12490"
     CDS             complement(301..1896)
                     /locus_tag="VE646_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HZD80840.1"
                     /translation="MNVLRVQGLAVQVAGRTLVEDLSLTLREGDTLGVVGRNGAGKTS
                     LLRVLAGEDAPLRGRIVRRGALGYLRQEPRRRGGEEAASGLSHLLQARDLERMAVKLE
                     KARLILEERPSPRNVARFSRLEERYRAVGGYSGEADARRIAAGLGLSDGRLELPMAAL
                     SGGERRRLDLAAILFGGSDLLLLDEPTNHLDGDAKAWLMSFLDSFRGALVVVSHDLAL
                     LDASITRILHLDRGAVVLYRGTYSRYRAAREADERRMAALAERQQAEIGRLRTLADSM
                     RGQGGKRARKAKTLDTRVERLRSHAVAAPARERTVRYRLPEPPHSGRDVLEARALCKS
                     YGGPAVIDDLSLGVSRGERLLVMGLNGAGKTSLLRLLAGVSPADSGAVRLGAGVRLGY
                     YAQEHEGIRAGLRVLDHLRVGSTATDQDLRTLLGMFGLSGSVAFRDAGSLSGGEKTKL
                     ALAQLVAGSCNLILLDEPTNNLDPPSRTAVAEALSRWPGTMIVVSHDTDFVSALAPQR
                     VLMMPEGTLEGWTDDLLELVALD"
     gene            complement(2385..2567)
                     /locus_tag="VE646_12495"
     CDS             complement(2385..2567)
                     /locus_tag="VE646_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrophobic protein"
                     /protein_id="HZD80841.1"
                     /translation="MLTAIVVLLLIAVLFGLGFVVKFLFWVALALLVIWAIGFFVGAG
                     AGAGARAGGRRRWYYW"
     gene            complement(2594..2767)
                     /locus_tag="VE646_12500"
     CDS             complement(2594..2767)
                     /locus_tag="VE646_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80842.1"
                     /translation="MGTTGSGAVLLSLCITVAVLAVATGMLFVSSLLERLDRPRTSRG
                     TRAWKDGPAARAR"
     gene            complement(2855..3793)
                     /locus_tag="VE646_12505"
     CDS             complement(2855..3793)
                     /locus_tag="VE646_12505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZD80843.1"
                     /translation="MSSSEDKTTVYVAMGANILIAVAKLFAGIISHSSALLAETAHSI
                     ADTMNQVFLRISLSLGGRPPDEEHPFGYGKERFFWAFMAAIFIFVSGAVFSVADGLYR
                     LLFSSAEQGSEFVAYLVLGVAFVAEGTSLVRAARQVRGEAAEAGQPFLRYIGMSKDPT
                     VKTVLLEDSAAVVGVVLATAGVLLHSLTGHRYWDAGASVAIGLLLAFVAYRLGTDTKG
                     LLLGKAATPDERERMRRTILAHEEISQVRQLLTMYVGPDSVLVAARVALRDGLDADAV
                     EDLADRIDRDLRREIPQITEVFVDPTPEAATRRRDG"
     gene            complement(3878..4495)
                     /locus_tag="VE646_12510"
     CDS             complement(3878..4495)
                     /locus_tag="VE646_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015783904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZD80844.1"
                     /translation="MGAVTVGALFSMPSDARGVVVFAHGTGSGRHSPRNNAVAQVLQG
                     AGLATLLIDLLSEEEERIDLRTGSLRFDIGFLAERLDAATRWLVERAEAQSLPVGYFG
                     ASTGAAAALVAAARHPEDVGAVVSRGGRPDMAAEALPEVRAPTLLIVGGLDTAVLEMN
                     REALEQLRCPKDLRVVPGASHLFEEPGTLERVSELAKEWFVQHLA"
     gene            complement(4528..4656)
                     /locus_tag="VE646_12515"
     CDS             complement(4528..4656)
                     /locus_tag="VE646_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80845.1"
                     /translation="MDERASGRAIPGAVATVMEPARTAGGEPAALPVESGGPDISQ"
     gene            4836..5177
                     /locus_tag="VE646_12520"
     CDS             4836..5177
                     /locus_tag="VE646_12520"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HZD80846.1"
                     /translation="MARLEGYRFGRIVVDGQEQTRDLIVLPNRVVTNWWRKDGHGLVL
                     DDLEEVLDELPPRLIVGAGALERLRPDPGTVQTLAERGIEVEVLPTPQAVARYTSSDH
                     AVVAAALHLTC"
     gene            5289..6248
                     /locus_tag="VE646_12525"
     CDS             5289..6248
                     /locus_tag="VE646_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD80847.1"
                     /translation="MIRRTLFMVLVLFIVSLVTFIIFVKLPSVDPAIRAAGRHPTPQL
                     LRTIRHQFGLDRPIWVQYWRFAKGLIPWPGWFLNREVYFSWSSRVAINEQIYSRLPVT
                     AALTIGSAAIWLLMGIPVGIVSAVKPGSLLDRSAMIAALVGVSAPDFWLGLVFLYVFH
                     FWLHVAPTSGIPIGMSTIGAVLHGYFVLPWLTLASTSAAFYSRMTRSNLMDSISQDYI
                     RTARAKGLSERRVIYKHGLRGALTPVVTMLGLDVAFLLGGAVVTESVFQLPGLGQYAL
                     QALYLSDFPAVMGVTVLGALFIVVANLVVDIVYAFLDPRVRYD"
     gene            complement(6261..>6720)
                     /locus_tag="VE646_12530"
     CDS             complement(6261..>6720)
                     /locus_tag="VE646_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80848.1"
                     /translation="LSYGPWGPDVTITGVHAQGTITHDRLEARIGRIRASSSIQPRLR
                     ERSILRARAGSRIHVEVTLQPLDGTDPIVSTLDLRVPRSARGDRPLRFRGGRDRGWVP
                     AASSLSELIMELNGGEHRNDLIVTGLGHGLSREQSMIVKGAATLTIRAIR"
BASE COUNT          957 a         2522 c         2242 g          999 t
ORIGIN      
        1 ctccagcccg cctgccggcg ggtgagcgtg ggcgtcctgg aagccgggca ggagcatccg
       61 cccctcgagc tcgacgacct ccgttcgggg gccgatcacc tcccgcaccg cttcatcggt
      121 gccgacggcc accaccttcc cgctgcggac cgcggccgcc tccgcccagc gccgcgccgc
      181 atcgaccgtg tagacggctc cgcccgtgaa gaccagatcg gcgctctcgc gtccctgcgc
      241 tcccatgccc atcacccctc gcgcccgggt cgccgcgagc ataccgaacg agcgaggcgc
      301 tcagtccagg gccacgagct ccagcaggtc gtccgtccac ccctccagcg tcccctccgg
      361 catcatgagg acgcgctgtg gcgcaagcgc cgagacgaag tccgtgtcgt ggctcaccac
      421 gatcatcgtg ccgggccacc gggagagggc ctccgccacg gcggtgcggg agggcgggtc
      481 gaggttgttg gtgggctcgt ccaacaggat caggttgcag cttcccgcca ccagttgcgc
      541 caaggccagc ttcgtcttct ccccgcccga gagcgagccc gcgtcccgga aggccaccga
      601 gccgctcagc ccgaacatcc ccagcagcgt ccgcaggtcc tggtcggtcg cggtcgaccc
      661 cacccgcagg tggtcgagca cccggagccc cgcccggatc ccctcgtgct cctgcgcgta
      721 gtagccgagc ctgaccccgg cacccagccg cacggcgccg gagtcggccg gcgagacacc
      781 cgccaggagc cggaggagcg aggtcttgcc cgccccgttc agccccatca ccaacagccg
      841 ctcccctctg gacacgccga ggctgagatc gtcgatgacc gccggcccac cgtaggactt
      901 gcacagcgcc cgggcctcga gcacgtcccg gccgctgtgc ggaggctcgg gcaaccggta
      961 ccggacggtc cgctcccgcg cgggcgcggc tacggcgtgc gagcggagcc gttccacccg
     1021 cgtatccagg gtcttcgcct tccgggcccg cttgccgccc tgcccccgca tcgagtcggc
     1081 cagggtgcgg agccggccga tctccgcctg ctgccgctcg gcgagcgccg ccatccgccg
     1141 ttcgtccgcc tcccgggccg cccggtagcg cgaatacgtg ccccggtaca gcaccacagc
     1201 gccccggtcc agatgcagga tccgcgtgat ggacgcgtcc agcagcgcca ggtcgtggct
     1261 caccaccacc agggctcctc ggaacgagtc caggaacgac atcagccagg ccttcgcgtc
     1321 gccgtccagg tggttggtgg gctcgtccaa caggagcagg tccgaaccgc cgaagaggat
     1381 cgcggccagg tccaggcgac gtcgctcgcc acccgagaga gccgccatcg gcagctccag
     1441 gcgcccgtcg gacagcccca agccggcggc gatcctccgc gcatccgcct cgcccgagta
     1501 ccctcccacc gcccggtacc gctcttccag ccgggagaat ctggcgacgt tccgtgggct
     1561 cggacgctcc tccaggatga ggcgtgcctt ctcgagcttg acggccatgc gttcgagatc
     1621 gcgggcctgc agcagatgtg acaggccgga cgcggcctcc tcgccacccc ggcggcgagg
     1681 ttcttgacga aggtagccca gcgctccccg ccgcacgatc cggccccgga gcggtgcgtc
     1741 ctccccagcc agcacccgga gcagcgaggt cttccccgcc ccgtttcggc cgacgacgcc
     1801 cagcgtgtcg ccctcgcgca gggtgagcga gagatcctcc accagtgttc gaccggcgac
     1861 ctggaccgcg agcccctgca cccggagcac gttcacgacc ggcctccggg agcggcggcc
     1921 agggcgaggg atggagagcg ggtcagcatg agatgggggc ctccgtggat gcagcggaac
     1981 cgggcgcagg cgcgccgagt gaatccgagc gaggttcgcc tatctcacga cgtcgccagt
     2041 gtaccgaggt ggcgaccagg tggccggggc gagcggccct ccgtatcctg acctgggcgg
     2101 gtacctacca gttcaccgtc tccggttggc ggtggctgtt cccgttgaac gtggggcggg
     2161 ggcgatcgcc gccgtcgggc tccctccata cgcgggcttc cgccaggagc ggcggttcga
     2221 cgccccggcc ggggcatcgg cacggcagga ctcgtgcgtc tggtcctcgg gccgtccgag
     2281 gccgaccggt ggtcccaccg ggtcgggacg ccgaccctca ccgacgtccc atccggcctc
     2341 cgctcaggcc gcgttcgacg acgtcttcct ggtcacctcc ggctctacca gtagtaccag
     2401 cgccggcgtc ccccggcccg ggcaccggcc cccgcgccgg ctcccacgaa gaacccgatg
     2461 gcccagatca ccaggagggc cagggccacc cagaacagga acttcaccac gaacccgagc
     2521 ccgaacagga cggcgatcaa cagcaggaca acgatggccg tcagcacagc agaccccctc
     2581 tccgtcagct ccgtcagcgc gcccgagcgg ccggaccgtc tttccaggcc cgggtgcccc
     2641 gagaggtgcg tggacggtcg agccgctcga gcagcgacga gacgaagagc atgccggtgg
     2701 ccacggccag gacggccacg gtgatgcaca gcgagagcaa cacggccccc gatcccgtcg
     2761 ttcccatgcc cgatcctcca gtccctcccc atcggatggt cgctcggatc tcgtgagggt
     2821 cgttcccctg cagggccttc aacaaaccct tcctctatcc gtctcggcgg cgggtggcgg
     2881 cctccggcgt ggggtccacg aacacctccg tgatctgggg gatctcacgg cggaggtcgc
     2941 gatcgatccg gtcggcgaga tcctcgacgg cgtcggcgtc gaggccatcg cgcagggcga
     3001 cccgcgcggc caccaggacc gagtccgggc cgacgtacat cgtgaggagc tggcgcacct
     3061 gcgatatctc ctcgtgcgcc aggatcgtcc ggcgcatccg ctcgcgctcg tctggcgtgg
     3121 cggccttgcc cagcaggaga cccttcgtgt ccgtgcccag ccggtaggcc acgaaggcca
     3181 gcaggaggcc gatcgccacc gaggctccgg cgtcccagta gcgatgaccg gtcaggctgt
     3241 gcaggagaac gcccgccgtc gcgagcacca cccccaccac cgccgcgctg tcctccagca
     3301 gcaccgtctt caccgtgggg tccttgctca taccgatgta cctcaagaag ggctgccccg
     3361 cctcggcggc ctcaccccgg acctgccggg ccgcccgcac cagcgaggtc ccttcggcca
     3421 cgaaggccac cccgagcacg aggtaggcga cgaactccga tccctgctcg gcgctcgaga
     3481 acaggagccg gtacagcccg tcggccaccg agaacacggc tcccgacacg aagatgaaga
     3541 tggccgccat gaaggcccag aagaaccgct ccttgccgta gccgaacggg tgctcctcgt
     3601 cgggggggcg gccgccgagg gacagggaga tccgcaggaa gacctggttc atcgtgtcgg
     3661 cgatcgagtg ggccgtctcg gccagcagcg ccgaggaatg cgagatgatc cccgcgaaca
     3721 gcttggccac ggcgatgagg atgttcgccc ccatcgccac gtacaccgtg gtcttgtcct
     3781 ccgacgacga catgcgtccc tcctccggcg cccgatgtat ccccgctcag agccggttcc
     3841 acgccccagg gccgatccgg gtggccggtg ggggatctca ggccaggtgc tgcacgaacc
     3901 actccttcgc gagctcgctg acccgctcca gcgtcccggg ctcctcgaag aggtgcgagg
     3961 ctcccggcac cacgcggagg tccttcgggc agcggagctg ctccagggcc tcacggttca
     4021 tctccagcac cgccgtgtcc agccccccca cgatcagcag ggtgggcgcg cggacctccg
     4081 ggagggcctc ggccgccatg tccggtcgtc cccctcggga caccaccgcc ccgacgtcct
     4141 cggggtgccg ggcggcagcc accagggcgg ccgcggcgcc cgtgctcgcg ccgaagtagc
     4201 ccaccggaag gctctgcgcc tccgcgcgtt cgaccagcca ccgggtggcg gcgtccaggc
     4261 gctcggccag gaacccgatg tcgaaccgga gcgagcccgt ccgcaggtcg atccgctcct
     4321 cctcctcgct cagcaggtcg atcagcaagg tggcgaggcc cgctccctgc agcacctggg
     4381 ccacggcgtt gttccgaggg ctgtggcggc cgctgccggt gccgtgcgcg aacaccacca
     4441 cacctcgagc gtccgagggc atggagaaga gggcgcccac cgtgacggcg cccatgtcga
     4501 cacgggcctc gtgcggttgg ggtgtcgtca ctgcgagatg tccggccctc cgctctcgac
     4561 cggcagggcg gcgggctcgc cgccggccgt ccgagccggc tccatcaccg tcgccacggc
     4621 accggggatg gcgcgtccgc tggctcgttc atccacagcc gatcctcctc tcctgccgga
     4681 gggatccctg catgggttgt tcccggacgg caggaggagg aatcgagggt ccgccgtcga
     4741 cctcgtctcc acggcatgcg tccccgcgcc gtggtatgga gtcaccgctc ccggggcatg
     4801 cttgatgatg caggctcgat ccgcgaaggg acgccatggc caggctggag ggatatcggt
     4861 tcgggcggat cgtggtcgac gggcaggagc agacccggga cctcatcgtg ctgccgaacc
     4921 gggtggtgac gaactggtgg cggaaggacg gccacggcct cgtgctcgat gacctggagg
     4981 aggtcctgga cgagctgcct cctcggctca tcgtgggcgc tggggccttg gagcgcctgc
     5041 gccccgatcc gggcacggtg cagacgctgg ccgagcgcgg catcgaggtg gaggtgctcc
     5101 ccacgcccca ggcggtcgcc cgctacacgt cctccgatca cgctgtggtc gcggcggcgc
     5161 tgcatctgac ctgctgaacc cccctctccg ggccgggcag atcgggccgt aggtctccca
     5221 tcgcgtgggt accatggacc ccccgccccc tccgggcaac gcggaagctg ctctcgatgc
     5281 tccggtacat gatccgacgc acgctgttca tggtcctggt gctgttcatc gtgtccctgg
     5341 tcacgttcat catcttcgtg aagctcccgt cggtcgatcc ggcgatccgc gccgccggcc
     5401 ggcacccgac ccctcagctc ctccgcacga tccgtcacca gttcggcctg gaccggccga
     5461 tctgggtcca gtactggcgc ttcgccaagg ggctgatccc gtggcccggg tggttcctga
     5521 acagggaggt ctacttctcc tggtccagcc gggtggccat caacgagcag atctacagcc
     5581 ggctgccggt gacggcggcg ctcacgatcg gctcggccgc gatctggctg ctgatgggga
     5641 tccccgtggg catcgtctcg gcggtgaagc ccggctcgct gctggaccgc tccgccatga
     5701 tcgccgcgct ggtcggcgtg tcggcgcccg acttctggct gggcctggtc ttcctgtacg
     5761 tcttccactt ctggctgcac gtcgcgccga ccagcgggat cccgatcggg atgtccacca
     5821 tcggcgcggt gctgcacggg tacttcgtgc tgccgtggct cacgctcgcg tccacctcgg
     5881 ccgccttcta ctcgcgcatg acccgcagca acctgatgga ctcgatctcc caggactaca
     5941 ttcgcacggc gcgcgccaag ggtctgtccg aacggcgggt gatctacaag cacgggctgc
     6001 ggggtgcgct cacgcccgtg gtcaccatgc tcggcctgga cgtggcgttc ctgctgggcg
     6061 gggcggtggt caccgagtcc gtgttccagc tccccgggct gggccagtac gcgctgcagg
     6121 ccctctacct gtccgacttc ccagcggtca tgggcgtcac ggtgctcggc gccctgttca
     6181 tcgtcgtggc caacctggtc gtcgacatcg tctacgcgtt cctggacccg cgggtccggt
     6241 acgactgaac ctcacgacgc tcagcggatc gctcggatgg tgagggtggc ggcccccttc
     6301 acgatcatgg actgctcgcg actcagtccg tggccgagcc ccgtcacgat caggtcgttg
     6361 cgatgttcac ctccgttcag ctccatgatg agctcgctca gggatgaggc ggccgggacc
     6421 catcctcggt ccctgccccc ccggaaccgg agcgggcgat cgccccgagc cgacctcggc
     6481 acccgcaggt ccagggtcga gacgatcggg tcggtcccgt cgagcggctg aagggtcacc
     6541 tccacgtgga tccgggaccc cgcccgcgcc cgcaggatgg accgctcccg gagcctgggc
     6601 tggatcgagc tgctggcacg gatccggccg atgcgggcct cgagccgatc atgggtgatg
     6661 gttccctggg cgtgcacccc ggtgatggtc acgtccggtc cccagggccc gtacgacagg
//
LOCUS       DASQQQ010000334         4857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_135248, whole genome shotgun sequence.
ACCESSION   DASQQQ010000334 DASQQQ010000000
VERSION     DASQQQ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135248"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="VE646_12535"
     CDS             <1..1133
                     /locus_tag="VE646_12535"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZD80849.1"
                     /translation="KGMDELAAALGFRVEVRIPLKLAFEEEDRKIVELTDDQAWILSF
                     IAHRKRAAVTGPAGSGKTLLALQIATRMAGAGHRTLLTCYNRRLAEHLRASTGDIPRL
                     EVRHFHDVCVRLAQEAGMELPDPTEDLDPDYFEHRLPEALKRAASRLGPRYGAVVVDE
                     AQDFRAWWWPALLALHEEPDDGFLYLFADDNQNLYEGGSLPIEPDAACPPLPANLRNT
                     RSIHEFISVFYEGASTSVGKGPTGRPPQILDYRNDEELVHLLTVVLRDLVDQEGVPLE
                     DIVVLTPSGRGKSRLRARARVDGFQLSETPGPDTVLTASVHGFKGLERPVVILAELQD
                     KHREELASYLYVGGSRARNHLIILAAEPVAKELRSLTGVTDD"
     gene            1126..2046
                     /locus_tag="VE646_12540"
     CDS             1126..2046
                     /locus_tag="VE646_12540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HZD80850.1"
                     /translation="MTEGLRAIHRCARNTGHHPLAAFEDAVGTVSNMGEDRWFFGPES
                     VSWRVHREVTVLFGGARALLMQAAHPLVIAGARETGMYERNPWRRLERTLVLTYSMTF
                     GTKSEASAAAERINEVHRRVNGVDPVTGLPYDALDPELLLWVHACLVDSALLFEELTV
                     GRLDQAGRQRFHEEQMLAAQMVLVPREIIPPTVPELRAYIRRVSESGILRMTDAAGTV
                     AGLFRHPPREAEWRPVLRGVSRLAFGTLPPSIRDLYGVPWSVPKRLAMNATFALTRAL
                     VPMLPSRYRFIAPYQEWRRRHRAAQAGSLH"
     gene            2055..2828
                     /locus_tag="VE646_12545"
     CDS             2055..2828
                     /locus_tag="VE646_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015577189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HZD80851.1"
                     /translation="MDVDGLLLDMDGVLTLSWQPLPGAEETVRWLRQRGLPFRVITNT
                     TTHPRSELAARLSGIGIEVEADEVVTAVVATAEYLRRHHPGAKVRLLSDGDASADLED
                     IRLVEEEPDVVVIGGAFEGFTYGALNRAFRAVMEGAVLLGMHRNLFWRTDEGWQLDGG
                     AYIAGLEEATGRRAVICGKPAAEFFGSALSTMGIEPARAAMVGDDVVSDVLGAKASGL
                     RGVLVRTGKFRAQDLQQATEQPDHVIGSIAALPELLASS"
     gene            2838..3569
                     /locus_tag="VE646_12550"
     CDS             2838..3569
                     /locus_tag="VE646_12550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZD80852.1"
                     /translation="MATERNRFAERLFSGIAPGYERVGAVMSIGQDPRWRRFLVSRIN
                     AIPGSWVLDVATGTGLVARELARRRNVHVVGLDQSEDMLLRGASGVAAVGLRERVSFV
                     RGRGEALPFPDGAFDGLTFTYLLRYVDDPAQTLRELSRVLRPGGVMAGLEFFVPVDPY
                     LRAGWKLYTRTVLPAVGWLSSPAWFRTGRFLGPNIEDFYRRFPLPTQVGMWQEAGISH
                     IRTRVMTLGAAVVTWGIKRRPGSDG"
     gene            3562..4296
                     /locus_tag="VE646_12555"
     CDS             3562..4296
                     /locus_tag="VE646_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80853.1"
                     /translation="MAESTERPAFYALASGGWRDYWTLLHPPYTVWHLSYVVIGTTLV
                     DRVNVRWLGETTAAFFLAVGVAAHALDELRGRPLRTSIPDRTLWALAAGGVAGAVALG
                     IHGALVLGPWTGAFIVVGGFLVIAYNLELLGGRFHSDLWFALAWGGFPALTGFFAQTG
                     TVTGAAVLVAAACVAFSAAQRVLSTPVRRLRRRVVRIEGSLQTGEGSSQPIDRRTLLA
                     APESGLRLLSLAMPLLAGGLLLARLI"
     gene            4354..>4857
                     /locus_tag="VE646_12560"
     CDS             4354..>4857
                     /locus_tag="VE646_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HZD80854.1"
                     /translation="MSRPATVGELRAAGFPDRSVKEELRVNLLAKLGRAEPLFPRIVG
                     FDDSVLPSLERGILAGHDLILLGERGQAKTRLIRGIIDLLDVDSPVITGCEVNDHPYR
                     PICARCRMLVSEEGDAVPISWMPRESRYAEKLATPDTSVADLIGDVDPIRVAEGRYLA
                     DELTIHYG"
BASE COUNT          677 a         1594 c         1771 g          815 t
ORIGIN      
        1 ccaaggggat ggatgagttg gctgcggcac tggggttccg cgtggaggtg cggatcccgc
       61 tcaagctcgc cttcgaggag gaggacagga agatcgtcga gctcacggac gatcaggcat
      121 ggatcctgtc gttcatcgcc caccggaagc gagccgccgt caccggtcca gccgggtcgg
      181 ggaagacgct cctcgccctc cagatcgcga cgcggatggc cggagcggga caccgtacct
      241 tgctgacctg ctacaacagg cggctggccg agcaccttcg ggcatcaacg ggcgacatcc
      301 cgcgcttgga ggtgaggcat ttccacgacg tgtgtgtgcg cctggcccag gaggcaggca
      361 tggagctccc cgatccgacg gaggatctcg accccgacta cttcgagcat cggctccccg
      421 aggcgctgaa gagggctgcg tcacgactgg gtcctcggta cggggccgtg gtggtagacg
      481 aggcgcagga cttccgggcc tggtggtggc ccgccctcct ggccctccac gaggagcccg
      541 atgacggctt cctgtacctg ttcgcggacg acaaccagaa cctgtacgag ggcggcagcc
      601 taccgatcga gccggatgcc gcctgccccc cgctccccgc gaaccttcgg aacacccgct
      661 cgatccacga gttcatctcg gtcttctacg agggggcttc gacgtcggtg gggaagggac
      721 cgacgggccg gccgccgcag atcctcgact accggaacga cgaggagctc gtccacttgc
      781 tgacggtggt gctccgagat ctggtcgatc aggagggtgt tcccctcgag gacatcgtgg
      841 tcctcacccc gtccgggcgc ggcaagagcc gcctgcgcgc cagggcgcgg gtggacgggt
      901 tccagctgtc cgagacaccg gggcccgaca ccgtgttgac ggcgagcgtc cacggcttca
      961 aggggctgga gcgacccgtg gtcatcctgg ccgagctgca ggacaagcac cgggaggaac
     1021 tcgcgagcta tctgtacgta ggcggctccc gtgcccggaa ccacctcatc atcctggccg
     1081 cggagccggt cgcgaaggaa ctgcgatcgc tgaccggcgt gacggatgac tgaggggctc
     1141 cgggcgatcc atcgctgcgc gcggaacacc ggccaccatc cgctggcggc gttcgaggac
     1201 gcggtcggta cagtttcgaa catgggtgaa gatcggtggt tcttcgggcc cgagagcgtc
     1261 tcctggaggg tccaccgaga ggtgaccgtc ctgttcggag gtgcgcgagc gctgctgatg
     1321 caggctgcgc accccttggt gatcgccggg gcccgggaga cgggtatgta cgagcggaac
     1381 ccgtggcgca ggctcgagcg aacgctcgtg ctcacctaca gcatgacgtt cgggacgaag
     1441 agcgaggcgt cggccgccgc agagcggatc aacgaggtgc accgacgggt gaacggcgtc
     1501 gatcccgtca cgggcttgcc gtacgacgcc ctggatccgg agctcctcct gtgggtccac
     1561 gcctgtttgg tcgacagcgc gttgctgttc gaggagctca cggtggggcg gctggaccag
     1621 gccggacgcc agcgcttcca cgaggagcag atgctcgccg cccagatggt cctggtgccg
     1681 cgtgagatca tcccgcccac cgtccccgag ctccgcgcgt acatccgccg cgtgtccgag
     1741 agtgggatcc tgcggatgac cgatgcggct ggcaccgtgg cggggctctt ccggcatccg
     1801 cctcgcgagg ccgagtggcg tccggtgttg cgaggagtct cccgtctggc gttcggcacc
     1861 cttccaccgt cgatccggga cctctacggt gtcccgtggt ccgtgccgaa gcgactcgcg
     1921 atgaacgcca ccttcgccct gacccgtgcc ctggtcccga tgcttccgtc ccggtaccgg
     1981 ttcatcgccc cctaccagga gtggcgtcga cgacaccggg cagcccaggc ggggtcacta
     2041 cactgacggc tccgatggac gtcgacggtc tgctcctgga catggacggg gtcctgaccc
     2101 tttcctggca gccgctgccc ggtgccgagg agacggtccg atggctccgg cagcgagggc
     2161 tcccgttccg cgtcatcacc aacaccacca cgcatcctcg gtcggagctc gcggcgaggc
     2221 tctccggcat cgggatcgag gtggaagccg acgaggtggt gacggccgtc gtggcgacgg
     2281 ccgagtacct gcgccggcat catccggggg ccaaggttcg actgctctcc gacggggacg
     2341 cctcggcgga tctggaagat atccggctgg tggaggagga gccggacgtg gtggtgatcg
     2401 gcggggcttt cgagggcttc acgtacggcg ccctgaaccg ggctttccgg gcggtcatgg
     2461 agggcgcggt gctcctcggc atgcatcgga acctgttctg gcggaccgat gagggatggc
     2521 agttggacgg tggcgcgtac atcgcgggac tggaggaggc gaccggtcgc cgggcggtca
     2581 tctgcgggaa gccggccgcg gagttcttcg ggtcggcact gtccacgatg gggatcgaac
     2641 cggctcgagc ggccatggtg ggggacgacg tggtctccga tgtgctcggg gcgaaggcgt
     2701 ccgggcttcg gggcgtgttg gtgcgcaccg ggaagttccg tgcccaggac ctccagcagg
     2761 caacggagca gccggatcac gtgatcggtt cgatcgcggc cctccccgag ctgctggcgt
     2821 cgtcgtagtc tccttccatc gcaaccgaac ggaaccgctt cgcggaacgt ctcttctcgg
     2881 gcatcgctcc cgggtacgag cgggtgggcg cggtcatgtc gatcggccag gatccccggt
     2941 ggcggcggtt cctggtctcg cggatcaacg cgatccccgg ttcttgggtg ctggacgtgg
     3001 ccaccggcac cgggctggtc gcgcgggagc tggccagacg ccgcaacgtc cacgtggtcg
     3061 ggttggacca gagcgaggac atgctccttc gaggcgcatc cggcgtggcg gccgtgggcc
     3121 tgagggagcg tgtgtcgttc gtgcggggac gcggcgaggc cctccccttc cccgacggag
     3181 ccttcgacgg cctgaccttc acgtatctcc tccggtacgt ggacgatccg gcccagacgt
     3241 tgcgcgagct ctctcgggtc ctgcggccgg gtggggtgat ggccggtctc gagttcttcg
     3301 ttcccgtgga tccctacctg agggcgggct ggaagctgta cacgaggacc gtgctcccgg
     3361 cggtcggatg gctctcctcg cccgcctggt tcaggaccgg gcgattcctg ggcccgaaca
     3421 tcgaggactt ctaccggcgg ttcccgctgc ccacgcaggt cgggatgtgg caggaggcgg
     3481 ggatctcgca catccgaacc agggtcatga cgctcggggc agccgtggtg acctggggga
     3541 tcaagaggcg gcccgggtcc gatggctgag tcgaccgagc ggcccgcctt ctacgcgctc
     3601 gccagcggag ggtggcggga ctactggacg ctgctgcatc cgccgtacac ggtctggcac
     3661 ctgtcgtacg tggtgatcgg gaccaccctg gtcgatcggg tgaacgtgcg ctggctgggg
     3721 gagacgacgg cggccttctt cctggccgtc ggggtggccg cgcacgccct ggacgagctc
     3781 cgcggccgtc ccctgcgtac ctcgatcccg gaccggacgc tgtgggccct ggccgcggga
     3841 ggggtggctg gggccgtcgc gctcggcatc cacggggccc tggtgctcgg tccctggacc
     3901 ggcgcgttca tcgtggtcgg tgggttcctg gtcatcgcct acaacctcga gctgctggga
     3961 ggtcgtttcc actccgacct gtggttcgcg ctggcatggg gtgggttccc agcgctgacg
     4021 gggttcttcg cccagaccgg cacggtgacc ggcgccgccg tgctggtggc cgccgcctgc
     4081 gtcgccttct cggcggcgca gcgggtcctg tcgacgccgg ttcgccggct ccgccgacgg
     4141 gtggtgcgga tcgaggggtc gctgcagacc ggcgagggtt cgagccagcc gatcgaccgg
     4201 cggaccctgc tggcggcgcc tgagtcgggg ctacggctgc tgtccctcgc catgccgctg
     4261 ctggccggtg gtctcctgct ggcccgcctg atctgatccc cggccggtcc tcgagctcca
     4321 ccattccgag gacgacccgt taccctggga gccatgagcc gaccggcaac cgttggtgag
     4381 ctccgcgccg ccgggttccc cgatcgctcc gtgaaggagg agctccgggt gaacctcctg
     4441 gccaagctgg ggcgggcgga gccgctgttc ccgcggatcg tggggttcga cgattccgtc
     4501 ctgccctcgc tggagcgagg catcctggcc ggtcacgacc tgatcctgct gggcgagcgg
     4561 gggcaggcca agaccaggct gatccgaggc atcatcgacc tgctggacgt ggactcgccc
     4621 gtgatcaccg ggtgcgaggt gaacgaccac ccctaccggc cgatctgcgc tcggtgccgg
     4681 atgctggtgt cggaggaggg cgatgctgtc cccatcagct ggatgccgcg tgagagccgc
     4741 tacgcggaga agctggcgac cccggacacc tcggtggcag acctgatcgg ggacgtggat
     4801 ccgatccgcg tggccgaggg ccggtacctt gccgacgagc tcacgatcca ttacggc
//
LOCUS       DASQQQ010000335         6599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_23604, whole genome shotgun sequence.
ACCESSION   DASQQQ010000335 DASQQQ010000000
VERSION     DASQQQ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23604"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VE646_12565"
     CDS             <1..294
                     /locus_tag="VE646_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZD80855.1"
                     /translation="LWRARSPIHSFDQMDRPLLVLQGLEDEVVPPDQAERMVRGMQVR
                     KLPYAYLAFEGEQHGFRKAESIQRSLEAELAFYAAILRIELPEPAPELQIHNR"
     gene            518..1129
                     /locus_tag="VE646_12570"
     CDS             518..1129
                     /locus_tag="VE646_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZD80856.1"
                     /translation="MLGYALAGIAVAVVVVLVILAMLPAGNSEASAVRPAGPGQVHTS
                     ASPRSSMLAKGDVVPSFSAPGFHMEATDGSGYVIKGERIGWSSYQGVPVVLVIWAPWC
                     PHCQAELPRLAEVAKDYPNVRLLTVVSAIGQEPGPTPNAYLAQHGLTFPVALDDAEGT
                     LLRILGIQGFPTLYFVNSDGTVAQASTGEIDESSLRLMMGNLS"
     gene            1229..1927
                     /locus_tag="VE646_12575"
     CDS             1229..1927
                     /locus_tag="VE646_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80857.1"
                     /translation="MDPVRTFGPRTSSRRCAMVILVLLLFVGGSWGWSAPAGATEGPT
                     VTGSVQGGTSVGDRLVIQVDATEPGGWRNLDRLDLDLLTGGTADRLSYDVRNQRLALN
                     QVSFVVQTGASARGNHLALDGATVVVTAGGADLALKIPTEVIATIPADARFRLRATDQ
                     LGRSGSVTLGVSAPPSGGVTWSTVAGLLLLALLVGGFVGNVTSSSRRSAPRFSVYGTI
                     RGKIERERRSAGVS"
     gene            2011..4095
                     /locus_tag="VE646_12580"
     CDS             2011..4095
                     /locus_tag="VE646_12580"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZD80858.1"
                     /translation="MWETLRVRSDIAEFRPQLASGTEVKRFHLRSGRDYAVVASPDGA
                     THYRLEPGEADLLRLMDGTRSVKEIVVERLRETGDLDLSGVADLVRSLQQGGLLTTRY
                     VDVPELVQGAMHRSPGVVAAAKRFATTLSIEWKSAHRSVAWTYRNLVRWVFTGPAAVV
                     TTGVAAIGFAGFLVVYASGRFSLSGREAVLDSLILMGLNYLLTFLHELAHAVVLVHYG
                     RRVKGAGFMLYFGSPAFFIDSTDGLLMEPRHRMLQSFGGPFAELVVAGLASLFVWLAP
                     GAPVSPILYKFALLNYFVIFLNLVPLLELDGYWILSDLIQVPDLRPRSLRFMRYDLWR
                     KLLARERFTRQEVGLTLYSLVGIAFTVFVVYLSVFFWIEVFGGLVSRLWGGGPLTRVV
                     LLALAVFLAGPVLRAGIAVLRSAARKVRALWGRVRFRFETSWRVEAGRLIDALPIFDD
                     LSEEVLSDLAGRVRLRNVPAGRPVVRQGERASAFYVIRRGTLEVVEEIGETGEERLLR
                     VLGKGESFGELGLSEGRVRSATVRAAEDSQLFEIDKSTFDRLLADMVHVPEFAPTIQA
                     IAELRELPIFSTLEPDELGELLEHGRWAQVPPGELIVEQGAVGDAFFVIGAGRVEVRQ
                     DGAVLRSLGPGAYFGEIALLMDVPRTASVTAVTPVRVFRLDREGFDRLVAEAFRKGTL
                     RPSSQADRTWQH"
     gene            4124..4339
                     /locus_tag="VE646_12585"
     CDS             4124..4339
                     /locus_tag="VE646_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014694478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80859.1"
                     /translation="MDCWHCRRTAVGTCRFCGRGICEDHVETMPYVLELYRGGEVTKA
                     LVVEDALYCGACTPRPDPLDLPELDSL"
     gene            4482..4610
                     /locus_tag="VE646_12590"
     CDS             4482..4610
                     /locus_tag="VE646_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80860.1"
                     /translation="MAEKKIAPKAATTKKASEKKEPQARVTKKVAMKVRTRRVRTR"
     gene            4710..5420
                     /locus_tag="VE646_12595"
     CDS             4710..5420
                     /locus_tag="VE646_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZD80861.1"
                     /translation="MVTAPAQTASLEALMAETPLLGSADPRSVHELVEVGHVRHFRRN
                     TYLFHQGDDCPDLYFLIEGRVEISSLSATGQRQLHTTIDRPQFFGELGVLGEVARTSS
                     ALALDETTAWAVPAEGFLGLVSRNPSVARAILRALARQVVAHEAFTDDLLFLDLKGRV
                     AKRLLQLVSPSLDDLPADGAVVPSVVTHADLASLCGGSRENVTRILSDFQRRGLVERD
                     GRRFVLRKISGLARLAGL"
     gene            complement(5452..6483)
                     /gene="moaA"
                     /locus_tag="VE646_12600"
     CDS             complement(5452..6483)
                     /gene="moaA"
                     /locus_tag="VE646_12600"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HZD80862.1"
                     /translation="MDTREWQPPTTPAPAEGPLVDTFGRVADDLRISVTDRCNFRCTY
                     CMPAEGLTWLPRRELLTFEELTRLLRIFLRLGVRSLKITGGEPTVRADLPVLIRMLRE
                     AGPDLDMSMTTNGVLLDRLAEPLAGAGLDRVSVSCDSLMRHRFREMTRRDALDRVLSG
                     IRAARDAGLTPIKINCVVIGGTNEEEVVDFARWARETGDVVRFIEYMPLDAERAWERA
                     KVVPSARIVEAVGRAFPLIAEGPDHEPATTYRFADGAPGGIGVIASVTEPFCDTCNRL
                     RLTAEGEVRSCLFALEETDLRGPMRSGASDQELEGLIRTAVWRKWSGHRINHPDFVQP
                     ERSMSMIGG"
BASE COUNT          905 a         2217 c         2397 g         1080 t
ORIGIN      
        1 ctgtggcggg cgcggtcccc gatccactcc ttcgaccaga tggatcgccc cctgctggtc
       61 ctgcaggggc tcgaggacga agtggtcccg ccggaccagg cggaacggat ggtccgcggc
      121 atgcaggtcc gcaagctgcc ctacgcctac ctggctttcg agggcgagca acacgggttc
      181 cgcaaggcgg agagcatcca acggtccctc gaggccgagc tggccttcta cgcggcgatc
      241 ctgcggatcg agctgccgga gccggcgccc gagctgcaga tccacaaccg ataggtcgga
      301 tccgtccccg cgccgaggtc gtcggcccgg cgccgggttc ccacatcgcc gtgcgtggcc
      361 cacatgccat cgaccgacga ggcgtggcac catgggcgga tgaggtccgc atcgccggag
      421 gatcaggatg tcgaacggtc ggtccagtaa gaaggatcgg cggcgggcag ccgctgaggc
      481 tcgacaggcc gccgttcgcc gtcaacgaca gcggcggatg ctcggatacg cgctggccgg
      541 catcgccgtc gcggtggtgg tcgtcctggt gatcctggcg atgctgcccg ctggcaactc
      601 cgaggcatcg gcggtgcgtc cggccggccc cggacaggtg cacacgtcgg catcgcctcg
      661 ttcctccatg ctggcgaagg gcgatgtggt gcctagcttc tccgcccccg ggttccacat
      721 ggaggccaca gacggcagcg ggtacgtgat caagggggag cggatcggtt ggtcctcgta
      781 ccagggcgtg ccggtcgtgc tggtgatctg ggccccctgg tgtccgcatt gccaggccga
      841 gcttccgagg ctggccgaag tcgcgaagga ctaccccaac gtccgcttgc tcaccgtggt
      901 ctccgcgatc ggtcaggagc ccggacccac gccgaacgcg tacctggctc agcacgggtt
      961 gacgttcccc gtggccctgg atgacgcgga gggcaccctg ttgcggatcc tggggatcca
     1021 ggggttcccc accctctact tcgtgaacag cgatgggacg gtggcgcagg cgtctacggg
     1081 agagatcgac gagtcgagcc tgcgattgat gatggggaac ctgtcgtgat cgtccgagcc
     1141 gactgatcct gggcctgcgc gccgcgaggt ccccgggccg ggccgatccg gttgcacgat
     1201 ccgtccggcg gcgtgaagat tcgcgcggat ggaccccgtc cgtacgttcg gtccacgcac
     1261 gtccagccgt cgctgcgcga tggtgatcct cgtgctcctc ctgttcgtgg gcggctcctg
     1321 ggggtggtcc gctccggccg gagccaccga aggacccacc gtgacgggaa gcgtccaggg
     1381 cggcacctcc gtcggcgacc ggctggtcat ccaggtggat gccaccgagc ccggggggtg
     1441 gaggaacctg gaccggttgg acctggacct gctcaccggt ggaaccgccg atcggctctc
     1501 ctacgatgtg cggaaccagc ggttggccct gaaccaggtc tccttcgtgg tccagaccgg
     1561 cgcctcagct cgtggcaacc acctggctct ggacggagcc accgtggtgg tcaccgccgg
     1621 tggagcggac ctggccctca agatccctac cgaggtcatc gcgacgatcc cggcggatgc
     1681 ccgattccgg ctgagggcca ccgaccagct tggtcggtcc ggaagcgtga cgttgggggt
     1741 ctcggctccg ccctcgggag gggtcacctg gtcgaccgtg gccggcctcc tgctcctcgc
     1801 gctcctggtc gggggcttcg tggggaacgt gacctcttca tcccgccgct ccgccccccg
     1861 cttctccgtg tacggcacga tccgcgggaa gatcgagcgg gaacgccgtt cggcgggcgt
     1921 gtcgtgagat ccgccaccgc tccgggcggg gtctgggccg cggtcgcggc ccgagccacg
     1981 gccccggcga gggggccgga ggatcgcggc ttgtgggaga ccctgcgtgt ccggtcggac
     2041 atcgcggagt tccgtccaca gctcgcgtcc ggcaccgagg tcaagcgttt ccacctgcgc
     2101 tcggggcgcg actacgcggt ggtcgccagc cccgatggcg cgacccacta tcggctggag
     2161 ccgggggagg cggacctgct gcgcctgatg gacggcaccc gatcggtgaa ggagatcgtg
     2221 gtcgagcgcc tccgggagac cggcgatctg gatctctccg gggtggccga cctggtccgc
     2281 tcgctccagc agggcgggct cctgaccacc cgatacgtcg acgtgccgga gctggtgcag
     2341 ggggcgatgc accggtcccc cggggtggtg gcggccgcga agcgcttcgc caccaccctg
     2401 tcgatcgagt ggaagagcgc ccaccgctcc gtggcctgga cctaccggaa cctcgtgcgg
     2461 tgggtgttca cggggccagc agccgtcgtg actaccggcg tggccgcgat cggcttcgct
     2521 gggttcctgg tcgtgtacgc atccggtcgg ttctccctgt ccggccgcga ggcggtgctg
     2581 gattcgttga tcctgatggg gctgaactac ctgctcacct tcctgcacga gctggcgcac
     2641 gccgtcgtgc tcgtgcacta cggccggcgg gtgaagggcg cggggttcat gctgtacttc
     2701 ggctccccgg ccttcttcat cgactcgacc gacgggttgc tgatggagcc caggcatcgg
     2761 atgctgcagt cgttcggggg gcccttcgcc gagctggtcg tggccgggct cgcgtccctg
     2821 ttcgtgtggc tggcacccgg ggcccccgta tcgccgatcc tgtacaagtt cgcgctcctg
     2881 aactacttcg tgatcttcct gaacctggtc ccgctgctcg agctcgacgg ctactggatc
     2941 ctctccgacc ttatccaggt accggatctg cggccccggt cgctgcgctt catgcgctac
     3001 gacctgtggc gcaagctcct ggcgcgggag cggttcaccc gacaggaggt cgggctcacc
     3061 ctctacagcc tggtggggat cgccttcacg gtgttcgtcg tctacctctc cgtcttcttc
     3121 tggatcgagg tcttcggggg cctggtctcg cggctgtggg gtggaggacc gctgacccgg
     3181 gtggtgctcc tggcactggc ggtgttcctc gcgggaccgg tgctgcgggc cggcatcgcg
     3241 gtcctgcgct cggccgcgcg caaggtccgg gccctgtggg gccgggtgcg ctttcgcttc
     3301 gagacgtcct ggcgggtgga ggcggggcgt ctgatcgatg cgctgccgat cttcgacgac
     3361 ctgtccgagg aggtgctgtc ggacctggcc ggacgcgtcc gcctgcgcaa cgtgcccgcc
     3421 ggccgaccgg tggtccggca gggagagcga gcctccgcct tctacgtgat ccgcaggggc
     3481 accctggagg tggtggagga gatcggcgag acgggcgagg agcggctcct gcgggtgctg
     3541 gggaagggcg agtccttcgg ggagctgggg ctgagcgagg gccgggttcg gtcggccacg
     3601 gtgcgggcgg cggaggacag ccagctgttc gagatcgaca agagcacctt cgaccggctc
     3661 ttggcggaca tggtgcacgt tccggagttc gcccccacga tccaggctat cgccgagctc
     3721 cgcgagctcc ccatcttctc cacgctggaa cccgacgagc tgggggagct gttggagcac
     3781 ggccggtggg cgcaggtgcc gccgggcgag ctcatcgtgg agcagggggc ggtgggtgac
     3841 gccttcttcg tgatcggagc cggtcgggtc gaggtccgcc aggacggggc ggtcctccgc
     3901 tcactcgggc cgggtgccta cttcggcgag atcgccctgc tgatggacgt gccgcgcacc
     3961 gcatcggtga ccgccgtgac gccggtgcgg gtgttccggc tggatcgtga ggggttcgat
     4021 cggctggtgg ccgaggcctt ccgcaaaggg acccttcggc cgtcgtcgca ggccgaccgg
     4081 acgtggcagc attagcaggc ccgatccgga aggggaccga tcgatggatt gctggcactg
     4141 tcgccggacc gccgtgggga cgtgtcggtt ctgcggacga gggatctgcg aggaccacgt
     4201 cgagaccatg ccttacgtgc tcgagctgta ccggggcggg gaggtcacca aggcgctggt
     4261 ggtggaggac gccctgtact gcggggcgtg cacgccccgg cccgacccgc tggacctacc
     4321 ggaattggac tccttgtgac cgatggcacc gaaggagtgt gacgtacgtc acctgggatc
     4381 gtgtgaccgg tatcacctcc ggccggtgac gggtggcacc gccgaccgcc ggcggcgatg
     4441 ccagcatagg ggcgtcagca gcccggaaag gggtatcgga gatggcggag aagaagatcg
     4501 ctccgaaggc ggccacgacc aagaaggcct ccgagaagaa ggagccgcag gcccgggtca
     4561 ccaagaaggt cgcgatgaag gttcggacca ggcgggtccg tacgcgctga tcctgttgcc
     4621 acgccgatcg tgtggacgga cccggagcca tccgggtccg ttgcacgttc ggtccgctgg
     4681 cttcctccac ttgaactagg ctgtggccga tggtgaccgc gcccgcccag accgcgtcgc
     4741 tggaagcgtt gatggccgag acgcccctcc tggggtcggc cgacccccgt tccgtccacg
     4801 agctcgtcga ggtcggccac gtccggcact tccgccggaa cacctacctg ttccatcagg
     4861 gcgacgactg ccccgacctg tacttcctga tcgagggacg ggtggagatc agctcactgt
     4921 ccgcgaccgg ccagcgccag ctccatacca cgatcgacag gccgcagttc ttcggagagc
     4981 tcggggtgct gggggaggtg gcccgaacct cctccgcgct ggcgctggac gagaccacgg
     5041 cgtgggcggt cccggccgag gggttcttgg gactggtttc gcgcaacccc agcgtggccc
     5101 gggcgatcct gcgcgcgctg gctcgtcagg tggtggcgca cgaggcgttc acggacgacc
     5161 tgctgttcct ggacctgaag ggccgtgtgg ccaagcgtct gctccagctc gtctcgccga
     5221 gcctggacga tctgccggcg gacggagccg tggtgccctc ggtcgtcacg catgcggacc
     5281 tagccagcct gtgcggtggg agccgggaga acgtgacccg gatcctctcg gacttccagc
     5341 ggcgcggctt ggtcgaacgc gacggccgcc ggttcgtcct acggaagatc tcgggcctgg
     5401 ctcgcctggc cgggctctga ggcgacgccg gcggccggct cggcgagggc ctcagccgcc
     5461 gatcatcgac atgctgcgct cgggctggac gaagtccggg tggttgatcc ggtggcccga
     5521 ccacttgcgc cagacggcgg tacggatcag cccctccagc tcctggtccg acgcacccga
     5581 gcgcatcgga ccacgcagat cggtctcctc cagggcgaac aggcaggagc gcacctcgcc
     5641 ctcggcggtc agccgcagcc ggttgcacgt gtcgcagaac ggttcggtca ccgaggcgat
     5701 cacgccgatc ccgcccggcg cgccatcggc gaaccggtag gtggtggcgg gctcgtggtc
     5761 ggggccctcg gcgatcagcg ggaaggcacg gcccacggcc tccacgatcc gggccgaggg
     5821 caccaccttg gcccgctccc aggcacgctc ggcgtccagc ggcatgtact cgatgaagcg
     5881 gaccacgtcc ccggtctcac gggcccagcg ggcgaagtcg acgacctcct cctcgttcgt
     5941 ccccccgatc accacacagt tgatcttgat cggcgtgagc ccggcgtccc tggcggcccg
     6001 gatcccggac aggacacggt cgagcgcgtc gcgccgggtc atctctcgga accggtgccg
     6061 catcagcgag tcgcacgaga ccgagacccg gtccaacccg gctccggcca gcggctccgc
     6121 caggcgatcc aggagcacac cgttcgtggt catggacatg tccaggtccg gccccgcctc
     6181 ccggagcatg cggatcagga cgggaaggtc ggcccgcacg gtcggctccc cgccggtgat
     6241 cttcagcgag cggaccccca gccggaggaa gatccggagc aggcgggtga gctcctcgaa
     6301 ggtgagcagc tcgcggcgcg gcagccaggt gagcccctcg gccggcatgc agtaggtaca
     6361 gcggaagttg cagcggtccg tcacggagat ccgcaggtcg tcggccacgc ggccgaacgt
     6421 gtccaccagc gggccctcgg cgggcgcggg ggtggtgggc ggttgccact cgcgcgtgtc
     6481 catcacctcc aggctacccg gatgtggccg ttcccagcta tgcctgctcg ggggccgact
     6541 cgggcaggag cgatcgcagg gtgtccatcg ccgcttccag ggcgtcggac cgacgggcg
//
LOCUS       DASQQQ010000336         4860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_51499, whole genome shotgun sequence.
ACCESSION   DASQQQ010000336 DASQQQ010000000
VERSION     DASQQQ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51499"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VE646_12605"
     CDS             complement(<1..407)
                     /locus_tag="VE646_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80863.1"
                     /translation="MFLVLALIAGVLASSSALADTSSRLDAAKRQLEDAKADLERATA
                     VWQEAEAKLTRTRNDIAATRVEIQRLQRTVDQVQGRLQRRAVVAFETGPAGTIDLLLS
                     SGSFTEFSDRMEFLGSMVQADSDLVVEGQVAGER"
     gene            complement(545..1744)
                     /gene="sat"
                     /locus_tag="VE646_12610"
     CDS             complement(545..1744)
                     /gene="sat"
                     /locus_tag="VE646_12610"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HZD80864.1"
                     /translation="MPQEGKDSMSATDPETIAPHGGRLVDLMVPEGERDHLSQQAEHY
                     PKLTVNHRELSDLEMLAVGALSPLTGFVGEKDYRSVLHTMHLESGVAWTIPVTLSLTE
                     EEVKRVGRADAVALASREGGDPLAVLQVDGVFKRDRQAESLGVFGTEDLEHPGVRALH
                     EAGDYCLAGSVQVLRLPRHRDFTRYRLTPQQTRAEFARRGWRTVVGFQTRNPIHRAHE
                     YIQKCALEIVDGLLVHPLMGATKADDVPPDVRMRCYEALFRDYYPKDRALISVFPAAM
                     RYAGPKEAIWHAICRKNYGCTHFIVGRDHAGVGSYYGTYEAQAMFDNFEPEELGITPL
                     RFEHAFYCRACEGMASPKTCPHEDEHRVILSGTKVREMLRNGERPPVEFSRPEVADIL
                     IEAMQAG"
     gene            1835..2578
                     /locus_tag="VE646_12615"
     CDS             1835..2578
                     /locus_tag="VE646_12615"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HZD80865.1"
                     /translation="MQILLADGDVRRLNERFESADPREVIGWALEEAGLERVAVASAF
                     QAEGTCVMHMASDIRPDIPILFLETGFHFAETLAFKQQLTELLGLNVVELVGEHTVES
                     QERALGPRLYERDPERCCEINKVRPMFEALRGLDAWITAFRRDSSPTRAQAPIVDRYE
                     LEPGRWMIKVNPVATWSRRDTWRYLRTHGLPHNPLYDLGYSSIGCAPCTRLRLPGEAE
                     RAGRWAGLSKWECGIHEHQTAGHGGPRGD"
     gene            complement(2627..3544)
                     /locus_tag="VE646_12620"
     CDS             complement(2627..3544)
                     /locus_tag="VE646_12620"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HZD80866.1"
                     /translation="MRRFQRVLLVVNPVARTVSRPTLAVIEKALSADFKLEVLETVER
                     GHATEVAAQAVMDGIDLVLVFSGDGTINEAVNGLAGTDVALGVLPGGATNILARSLEM
                     PLDPVDATGVLISKALDGEARKLHLGRAAGRYFAVNCGAGVDAAAMARLDSRFPSTKE
                     AFERAAFRAVARELLARYAGRSPDLSVRVDGGEALPSLSVMIGRTDPYTYYREHGVRV
                     TPAASLESGLDVLSARRLPRRRVVRIALQMFGSARHVRGRDMDYIHDASQVQVGSRAP
                     FPVQVDGDVMGEHTRLEVTLARDALWVVA"
     gene            complement(3553..3822)
                     /locus_tag="VE646_12625"
     CDS             complement(3553..3822)
                     /locus_tag="VE646_12625"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZD80867.1"
                     /translation="MTTVSVRLFAALRDLAGAARVQADGATVRDVVSALGERYGDRFA
                     QIAAAGSAVVDGERADPDRELAPGEELALLPPVSGGSFRPSLLGY"
     gene            3874..4533
                     /locus_tag="VE646_12630"
     CDS             3874..4533
                     /locus_tag="VE646_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007632135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD80868.1"
                     /translation="MAAVVLLSDRSPSEVLPALAELALDLKLLPLSLAEVPLALAVGP
                     EAVMVDAAESPGQGLAVLRKLREAGATPPAIALLERRDLERLPWDQSADEILFPSTSA
                     AEVRLRLSMLRRRWGIGEGSTIRLGPMILDTDGYRVVVSGRALDLTYKEFELLRHLAS
                     HPGRVFTRPTLLREVWGYDFYGGTRTVDVHVRRLRAKLGPEHEHLIGTVRGVGYRAAD
                     L"
     gene            4530..>4860
                     /locus_tag="VE646_12635"
     CDS             4530..>4860
                     /locus_tag="VE646_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005272170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZD80869.1"
                     /translation="MTSRPLEKASGSPVALPSETEVLVVGGGFAGLTAARELGRAGVR
                     VVLLEARDRLGGRTWTTSVQGSEVELGGAYVHWYQPHLWSELTRYGLGIQSGREPETA
                     AWLSEGRR"
BASE COUNT          714 a         1773 c         1666 g          707 t
ORIGIN      
        1 cgctccccgg cgacctgccc ctcaacgacc agatcggagt ccgcctggac catgctgccc
       61 aggaactcca tccggtcgga gaactcggtg aacgaccccg aggacaggag caggtcgatc
      121 gtgccggccg ggcccgtctc gaaggcgacc accgctcgcc gctgcagacg tccctggacc
      181 tgatcgaccg tgcgctggag tcgttggatc tccacccggg tggcggcgat gtcgttccgg
      241 gttctggtca gcttcgcctc ggcctcctgc cagacggcgg tggcgcgttc cagatcggcc
      301 ttcgcgtctt cgagctgccg cttggcggcg tcgagccgtg acgatgtgtc cgcgagggcc
      361 gaggacgagg cgagcacccc cgcgatcaac gcgaggacca ggaacacccg gagccagctt
      421 cgcggcgacc tcgtcggctg cccccttcgg tcatcccctc ggcgacccga gcccgggaga
      481 ggctaccaga gcccctgagc aggacgatcg cgcgccgatg acacggagcg cgctcccgag
      541 atccctatcc ggcctgcatg gcctcgatca ggatgtcggc cacctccggc cgcgagaact
      601 ccaccggcgg gcgctccccg ttccggagca tctcccggac cttggtcccg gacaggatca
      661 cccggtgctc gtcctcgtgg gggcaggtct tgggcgaggc catgccctcg catgctcggc
      721 agtagaaggc gtgctcgaac cgcagcggcg tgatgccgag ctcctccggc tcgaagttgt
      781 cgaacatcgc ctgtgcctcg taggtgccgt agtaactgcc cacgccggcg tggtctcggc
      841 ccacgatgaa gtgggtgcag ccgtagttct tccggcagat cgcgtgccag atcgcctcct
      901 tcggccccgc gtaccgcatg gccgccggga acacggagat gagcgcccgg tccttggggt
      961 agtagtcacg gaacagcgcc tcgtagcagc gcatccgtac gtccggaggg acgtcgtccg
     1021 ccttcgtggc acccatcagg ggatgcacca acaacccatc cacgatctcc agggcgcact
     1081 tctggatgta ctcgtgcgcc cggtggatgg ggttccgggt ctggaacccc accacggtcc
     1141 gccatccccg gcgggcgaac tcggcccggg tctgctgggg ggtgagccgg tagcgcgtga
     1201 agtctcggtg ccgaggcaga cgcaggacct gcaccgaccc ggccaggcag tagtcaccgg
     1261 cctcgtgcag cgcccgcacc cccgggtgct cgagatcctc ggtcccgaac acgccgaggg
     1321 actccgcctg gcgatcacgc ttgaagaccc catcgacctg cagcaccgcc aggggatcgc
     1381 cgccctcgcg cgaggccaat gcgacggcgt cggcgcgacc cacgcgcttc acctcctcct
     1441 cggtgagcga caaggtcacc gggatcgtcc acgccacgcc gctctccaga tgcatcgtgt
     1501 gcaggaccga ccggtagtcc ttctccccca cgaacccggt caacggggac aaggcgccca
     1561 cggccagcat ctccaggtcg gacagctcgc ggtggttgac cgtgagcttc ggatagtgct
     1621 cggcctgctg ggagaggtgg tcgcgttcgc cctcgggcac catcagatcg acgagccgac
     1681 ccccgtgggg cgcgatggtc tcgggatcgg ttgcagacat gctgtccttc ccttcctgcg
     1741 gcatccgggc gaacagcata gcgacggccg gccgaggctc tgccacgccc ccgatatact
     1801 gggttgcccg cgagcgcaaa ggcggacacc ggtcatgcag atcctgctcg ccgacgggga
     1861 cgtacgccgg ctgaacgaac gcttcgagtc cgcggaccct cgggaggtca tcggctgggc
     1921 tctggaggag gccgggctgg agcgcgtcgc ggtggcatcc gccttccagg cggagggcac
     1981 gtgcgtgatg cacatggcca gcgacatccg gccggacatc cccatcctgt tcctggagac
     2041 gggcttccac ttcgccgaga ccctcgcgtt caagcagcag ctcaccgagc tgctcggtct
     2101 gaacgtggtg gaactggtcg gagagcacac cgtcgagtcg caggagcggg cgctcggacc
     2161 ccgtctgtac gaacgcgatc ccgaacggtg ctgtgagatc aacaaggtgc ggcccatgtt
     2221 cgaggcgctc cgggggctcg atgcgtggat cacggccttc cggcgcgatt cctcccccac
     2281 ccgggcgcag gccccgatcg tcgaccgcta cgagctcgag ccggggcgtt ggatgatcaa
     2341 ggtgaacccc gtggccacct ggagcaggcg ggacacctgg cgatacctgc gcacgcacgg
     2401 cctgccccac aaccccctgt acgacctggg ctactcttcg atcggctgcg ctccgtgcac
     2461 ccggctccgc ctacccggag aggcggagcg cgccgggcgc tgggcggggc tctccaagtg
     2521 ggagtgcggc atccacgagc atcagaccgc cggccacggc ggaccgcgcg gcgactgata
     2581 cggacctccc gccgctcggc ggcggaaggg cggggccggc agggccctag gcgaccaccc
     2641 agagggcgtc tcgcgccaac gtgacctcga gccgggtgtg ctcgcccatc acgtcgccgt
     2701 ccacctggac ggggaacggg gcccgcgagc cgacctgcac ctgggaagca tcgtggatgt
     2761 agtccatgtc ccgtccgcgc acgtgcctgg ccgatccgaa catctggagc gcgatgcgca
     2821 ccacccggcg gcgaggcagc cgtcgggcgg acaggacgtc caagccggac tcgagcgacg
     2881 ccgcgggggt cacccgtacg ccatgctccc ggtagtaggt gtacgggtcg gtccgaccga
     2941 tcatcaccga gagcgagggc agcgcctcac cgccgtccac ccgcaccgag aggtcggggc
     3001 tccggcccgc gtatctggcc agcagctctc gggccaccgc ccggaacgcg gcccgctcga
     3061 aggcctcctt cgtggacggg aaccgagaat ccagccgcgc catcgccgct gcgtccacgc
     3121 cggccccgca gttgaccgcg aagtaccgac cggcggcgcg tcccagatgc agtttgcggg
     3181 cttccccgtc cagcgccttc gagatcagga ccccggtcgc atccaccggg tccagcggca
     3241 tctccaggga ccgggcgagg atgttggtgg ctcctcccgg cagcaccccg agggccacgt
     3301 cggtcccggc cagcccgttc accgcctcgt tgatcgtgcc gtcccccgag aagaccagca
     3361 cgaggtcgat gccgtccatc acggcctgcg ctgccacctc cgtggcgtgt ccccgctcca
     3421 cggtctcgag cacctcgagc ttgaagtcgg ccgacagcgc cttctcgatg accgcgaggg
     3481 tgggccggga caccgtccgg gccacggggt tgaccacgag cagcacccgc tggaatcggc
     3541 gcatggagac cctcagtatc ccagcagcga tggccggaag ctgcccccgg agaccggcgg
     3601 cagcagcgcg agctcctccc cgggcgccag ctcacgatcc ggatcggctc gctcgccgtc
     3661 cacgaccgcc gagccggcgg cggcgatctg cgcgaagcga tcgccgtagc gctcgccgag
     3721 cgcgctcacc acatcgcgta ccgtggcccc atcggcctgc acgcgcgcag cgccggccag
     3781 gtcccgcagg gccgcgaaca agcggaccga tacggtcgtc atcgcaggtc aagggtaccc
     3841 tcgccgtcga acggctggta ccctccggag gtcgtggcgg ccgtcgtcct gctctccgac
     3901 cgatccccct ccgaggtcct ccccgccctc gcggagctgg ccctcgacct caagctgctg
     3961 cccctgtccc tcgccgaggt ccccctggcg ctggcggtgg gtccggaggc ggtgatggtg
     4021 gacgcggccg agagccccgg ccagggcctg gccgtcctgc ggaagctccg ggaggccggg
     4081 gccaccccac cggccatcgc tctgctcgag cgccgggatc tggagcgcct accttgggat
     4141 cagagcgccg acgagatcct gttcccgtcc acctcggcgg ccgaggtccg tctccgcctg
     4201 tcgatgctgc gtcgccggtg gggcatcggc gaggggtcga cgatccgcct cggtccgatg
     4261 atcctggaca ccgacggcta ccgtgtcgtg gtgagcggcc gggccctgga cctcacgtac
     4321 aaggagttcg agctgctccg ccacctggcc tcacacccgg ggcgggtctt cacccggccc
     4381 acgctcctgc gcgaggtctg gggctacgac ttctacgggg ggaccaggac ggtcgacgtg
     4441 cacgtccgcc gcctgcgggc caagctcggt cccgagcacg agcacctgat cgggaccgtg
     4501 cgcggcgtgg gctaccgggc cgcggatctg tgacctcccg gccgctcgag aaggcgtccg
     4561 ggagcccggt tgccctcccg tcggagaccg aggtcctcgt ggtgggggga ggcttcgccg
     4621 gcctcaccgc ggcgcgcgag ctgggacggg ccggggtgag ggtggtgctc ctggaggccc
     4681 gcgatcgcct gggcggtcga acctggacca cctcggtcca agggtcggag gtggagctgg
     4741 gcggggccta cgtgcactgg taccaacctc acctgtggag cgagctcacc cggtacgggt
     4801 tgggcatcca gtccggccgg gagcccgaga cggctgcctg gctgtccgag ggccggcgcc
//
LOCUS       DASQQQ010000337         8164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_378027, whole genome shotgun sequence.
ACCESSION   DASQQQ010000337 DASQQQ010000000
VERSION     DASQQQ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8164
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378027"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..933)
                     /locus_tag="VE646_12640"
     CDS             complement(22..933)
                     /locus_tag="VE646_12640"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HZD80870.1"
                     /translation="MRKAAISIIGFLALAGVIGYLATSGGGTFRSADNGGGGAASEPL
                     AAASRAPGLGGDTAAGASVEGPTKSSANVGAVPAIGPAVVKTAQLEVQVAKDGFMAAF
                     GSAGTIAAKYGGYVQSSSTSGTKVHSGSLTIRVPAASFENAMSDLGELGTVKAQSMSG
                     TDVTSQYVDLHARLITWQAQEKVLLRLMRRSTSVEATLRVQQQLQDVQFRIEQLKGQL
                     RVLQDQTSLATIQLELREPGAPISTPRTVSERPSLGEAWDKALNGFFSVVYSVTVGLG
                     YLVPIALIVALGWLGYRRLRPRPVASS"
     gene            complement(993..1886)
                     /locus_tag="VE646_12645"
     CDS             complement(993..1886)
                     /locus_tag="VE646_12645"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HZD80871.1"
                     /translation="MSTGGTGHPADPRRSLLDWYGPRRRAYPWRRTRDPYRIWVSEIM
                     LQQTQASRVVPAYRRFLRRFPTLASLASAPSGAVIAAWDGLGYNRRAVALSRAARMIV
                     ESGGGIPSDPTALERLPGIGPYTAAAVASIAFGLSIPAVDTNVRRIVARVSVGADPRE
                     VPSWRIAELAGRWIAAEDPGGWNQALMDLGREVCRPRPRCAECPLRASCRFADIDHPA
                     APGRGRSSRFEGSTRQVRGAVVRSLRGSPGATLGAISARTGFSLTRVVEVVAALSSDG
                     LIHAGPAALEGRPAGRVRLPD"
     gene            complement(1905..2093)
                     /locus_tag="VE646_12650"
     CDS             complement(1905..2093)
                     /locus_tag="VE646_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80872.1"
                     /translation="MAREIDWALFEKAVDITASAVRGAMGGENSQPPKFAAEVFREVW
                     GALKEAVDELPEKGKAGF"
     gene            complement(2169..3341)
                     /locus_tag="VE646_12655"
     CDS             complement(2169..3341)
                     /locus_tag="VE646_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019355336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZD80873.1"
                     /translation="MAPAQIDPLDYLGLDHLLGDEERAIRDTVREWVGDRVLPEIEGW
                     FERGEFPLQVAKELGGMGLLGMHLTGYGCAGANAVSYGLVCMELEAGDSGFRSFVSVQ
                     GSLCMFPIRRYGSDEQKERWLPPMARGEVIGCFGLTEPDAGSDPARMRTVAHRDGAGW
                     VLSGTKMWITNGGIADLAVVWARTDEGVRGFLVPADTPGFSTRDVHRKLSLRASVTSE
                     LVLDEVHLPDDAVLPGATGMRGPLSCLTEARYGIVWGAMGAARTCFATALGYARTREQ
                     WGRPIGGFQLTQEKLANMALELSKGSLLALHLGRMKDEGRLRPQHVSLGKLNNVREAL
                     GIAREARSILGASGITLEYPVIRHMNNLESVYTYEGTNEIHTLIVGEDLTGLRAYQ"
     gene            3459..3827
                     /locus_tag="VE646_12660"
     CDS             3459..3827
                     /locus_tag="VE646_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010568550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZD80874.1"
                     /translation="MRYLLLIYGDEKATAATPPEELEVSRKAWMDYDGWLREQGWRRA
                     GDALQETSTATTVREKGGTIVTTDGPFAETKEQLGGYYLIECEDLDDAIQAASRMPTM
                     GRGSVEIRPVRDVGMRNEPS"
     gene            3848..5068
                     /locus_tag="VE646_12665"
     CDS             3848..5068
                     /locus_tag="VE646_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZD80875.1"
                     /translation="MDRLFRQEGGRAVATLIRVLGDFDLAEDAVQEAFALALETWPAR
                     GTPEKPGAWITRTAKNRAIDRLRRERRLREKTETLRQLAELEGLDEDIDMMGIPDDRL
                     RLIFTCCHPALSMEGRVALTLRTLGGLTTAEIARAFLVAEPTMAQRLVRAKRKIRDAG
                     IPYRVPSPELLPERLPTVLAVLYLIFNEGYASTSGPLVRSELCGEAIRLARLTDELLP
                     GQPEVLGSIALMLLHHSRRDARTGADGQIVLLQDQDRSRWDHSMIDEGLAVLDTALGL
                     GGPGPYQLQAAIAALHARAPRPEDTDWPQIAALYGGLIQMVPSPVVELNRAVAVAMAD
                     GPQRGLDLLEPLGPELDAYHLFHAARADLLRRLDRPGEAARAYRRARELASNPAERSF
                     LDHRLRELTGRPGA"
     gene            complement(5130..6518)
                     /locus_tag="VE646_12670"
     CDS             complement(5130..6518)
                     /locus_tag="VE646_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HZD80876.1"
                     /translation="MAERIERTRFDLSPEEVPPAWYNILPEMAGAGIGLLPPLHPETK
                     QPVTPDLLAPLFPEPLILQEVSTDPWIDAPGAVLDVYRLWRPTPLHRAVRLEQALDTP
                     AHIYFKYEGVSPAGSHKPNTAVAQAYYNKQAGIRRISTETGAGQWGSSLSMACRFFDL
                     QCLVFMVKASFDQKPYRRIFMETFGAEVRPSPSSTTESGKKILEDHPDSTGSLGIAIS
                     EAIEVAATNPDTNYSIGSCLNHVLLHQTVVGLEARRQMEMTGEQPDVVVGCVGGGSNY
                     GGLAYPFMGDKLRGERSDMRFLAAEPAACPTLTKGRFAYDFGDTAGLTPLLPMYTLGH
                     GFVPAPVHAGGLRYHGDSPSLSLLVREGYMEAAAYAQNEVFEAAVLFAQTQGIVPAPE
                     SAHAIRGAVNEAIAAREAGRERVILLGLSGHGHFDMSAYDDYLHGRLPEVEFDPGDEE
                     AALQGLPQVPID"
     gene            complement(6597..8027)
                     /gene="zwf"
                     /locus_tag="VE646_12675"
     CDS             complement(6597..8027)
                     /gene="zwf"
                     /locus_tag="VE646_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZD80877.1"
                     /translation="MGANLPPQAVVIFGASGDLTKRKLLPAFYSLFLQGLLPKGFAIV
                     GYSRTTLSDDDFRAIAHDGVKEYGEHAPHGEVWRDFAAHLSYLPGEFSDPGSMNHVIG
                     HLEGIDREQGTEGRRFFYAATPPVAYPDVVRRVAETGMHRDAKIVFEKPFGRDLRSAR
                     ELNAAIHEVFDESQVFRIDHYLGKETVQNVLALRFSNGLFEPVWNRRYIDHIQITVAE
                     DIGIEGRGAFYEETGAIRDMIATHLFQVMTFVTMEPPVSFEPDRLRDETVKVLRSTMA
                     CDPAKVVRGQYRGYRGEPDVADDSTVETFAALELEVDNWRWSGVPVYLRTGKELAAKC
                     SEVTLTFRKVPYNVFRGAEVDIPPRDHLTIRIQPNEGVTMAVNAKTPGPGLDLGRATI
                     DFDYEEEFGGRIADAYELLLLEAMEGDRTLFLRQDDVERSWAILDPILTDPSPVQRYE
                     PGTWGPPAADALIHPRRWHVTRAHRR"
BASE COUNT         1255 a         2910 c         2807 g         1192 t
ORIGIN      
        1 aatgacccag cggttcctgc gtcaggacga cgcgaccgga cgcgggcgaa gccgtcggta
       61 gccgagccag ccgagagcca cgatgagggc gatcgggacc aggtatccca gacccaccgt
      121 caccgagtac acgacgctga agaacccgtt cagggccttg tcccacgctt cacccaggct
      181 gggccgctcg ctcaccgtgc gcggggtgct gatgggagcg ccgggctcgc gaagctccag
      241 ttggatcgtg gccagggatg tctggtcctg gagcacccgg agctgcccct tgagctgctc
      301 gatccggaac tgcacatcct gcagctgctg ctggacccgc aacgtggctt ccaccgaggt
      361 ggagcgccgc atgagcctga gcaggacctt ctcctgggcc tgccaggtga tcagccgggc
      421 gtgcagatcc acgtactggg acgtgacgtc ggttcccgac atcgactgcg ccttgacggt
      481 cccgagctcc cccaggtcgg acatggcgtt ctcgaagctc gccgccggca ctcggatggt
      541 gagggatccg gagtggacct tcgtcccgga cgtggacgag gactggacgt agcccccgta
      601 cttcgccgcg atcgtgccgg cggaaccgaa ggcggccatg aagccgtcct tggccacctg
      661 cacctccagc tgggccgtct tcactacggc ggggccgatg gccggcaccg ctccgacgtt
      721 ggccgaggac ttcgtcgggc cctcgacgga cgcgcccgcc gccgtatcgc cacccaggcc
      781 gggggcccgc gaggccgcgg cgaggggctc tgaggccgcg ccaccgcccc cgttgtccgc
      841 cgatcggaac gtcccgcccc ccgaggtcgc caggtatccg atcacgccgg ccagggccag
      901 gaacccgatg atggagatcg ccgccttgcg catgagtacc tccctccgtg gatgcggcgg
      961 ttggacgcgg agggaggtcg acgcggttcc cgtcagtctg gcagccgtac ccggccggcg
     1021 ggccgacctt cgagggccgc cggccccgcg tggatcaggc cgtcggatga cagggcggcg
     1081 accacctcca ccacgcgcgt gagcgagaag cccgtccgtg cggagatcgc gccgagcgtg
     1141 gctccagggg atccccggag cgagcgcacg acggccccgc gcacctgccg ggtggatcct
     1201 tcgaacctcg aagaccggcc tcgtccaggc gcggcagggt ggtcgatgtc ggcgaagcga
     1261 caggacgcgc ggagggggca ctcggcgcag cgaggccgag gccggcagac ctcccgcccg
     1321 aggtccatca gcgcctggtt ccagcccccg gggtcctccg ccgctatcca ccggccggcg
     1381 agctcggcga tccgccagga cggcacctcc cgtgggtcgg cccccacgga tacccgggcc
     1441 acgatccgcc gcacgttcgt gtccaccgcg gggatcgaaa gcccgaatgc gatcgacgcg
     1501 acggccgccg ccgtgtacgg accgatcccg ggcagccgct ccaacgcggt ggggtcggac
     1561 gggatgccac caccactctc gacgatcatc ctggcggccc gggacagagc cacggcccgg
     1621 cggttgtacc cgagaccgtc ccacgcggcg atcaccgcgc cgctgggggc cgaggccagg
     1681 ctggcaagcg tggggaagcg tcgcaggaac cggcggtagg ccggaaccac cctggatgcc
     1741 tgggtctgtt ggagcatgat ttcggagacc cagatccggt aggggtcccg ggtccgccgc
     1801 caggggtatg cccggcgcct ggggccgtac cagtcgagca gcgaacggcg cgggtcggcc
     1861 gggtgcccgg tccctcccgt ggacaccgga tgccgagcgg ctagctagaa gcccgccttg
     1921 cccttctcgg gaagctcgtc cacggcctcc ttcagcgcgc cccacacctc gcggaagacc
     1981 tcggcggcga acttcggagg ctggctgttc tcaccaccca tcgctccccg gaccgccgac
     2041 gcggtgatgt cgacggcctt ctcgaacagt gcccaatcga tctcacgcgc catgatgtca
     2101 gcctcctggt tctcgcaggg atacggctcg agcgatcgac aggctaccgg acaccggacc
     2161 gatcggagtc actggtaggc ccgcaagccg gtcaggtcct cccccacgat cagggtgtgg
     2221 atctcgttcg tgccctcgta ggtgtagacg gactcgaggt tgttcatgtg gcggatcacc
     2281 gggtactcga gcgtgatgcc actcgcaccc aagatgctcc gagcctcgcg cgcgatgccc
     2341 agagcctccc ggacgttgtt cagcttcccc agtgagacat gctgcgggcg aagacgtccc
     2401 tcgtccttca tccggccgag atgcagcgcc agcaacgacc ccttcgacag ctcgagggcc
     2461 atgttcgcga gcttctcctg cgtgagctgg aagccgccga tgggtcgccc ccactgctcg
     2521 cgcgtccgtg cgtatcccag ggcggtcgcg aagcaggtac gcgccgcccc catcgccccc
     2581 cacacgatcc cgtagcgcgc ctcggtgagg caggagaggg gccctctcat gcccgtggct
     2641 cccggcagca cggcgtcgtc cgggaggtgg acctcgtcca ggacgagctc ggaggtcacc
     2701 gaggctcgga gcgacagctt gcgatgcacg tcccgggtgg agaaccccgg ggtgtcggcg
     2761 gggaccagga accctcgcac cccttcatcg gttcgggccc acaccacggc gaggtcggcg
     2821 atccctccgt tcgtgatcca catcttcgtc ccggacagga cccaccccgc cccatcccgg
     2881 tgggccacgg tccgcatccg ggccgggtcg ctgccggcat cgggttcggt cagaccgaag
     2941 cagccgatca cctcgcctcg ggccatcggc ggcagccagc gctccttctg ctcgtcgctc
     3001 ccgtagcgcc ggatcgggaa catgcacagc gatccctgga ccgacacgaa gctgcggaac
     3061 ccgctgtcgc cggcctcgag ctccatgcag accaggccgt aggagaccgc gttcgcaccg
     3121 gcgcacccgt accccgtcag gtgcatgccg agcagtccca tgccgcccag ctccttggcc
     3181 acctgcaggg ggaactcgcc gcgctcgaac cagccctcga tctccgggag aacccggtcc
     3241 cccacccact cccgaacggt gtcgcggatg gcccgctcct cgtcgccgag caggtggtcc
     3301 aggcccaggt agtcgagcgg gtcgatctgg gcgggggcca tgactcgcct agcctaacct
     3361 caccgtgatc gcggggccga tgtgtcgatc ctccggttcc ccgttcgtcg ttagggtgag
     3421 ctggcgacgg tgggtcgccg gagcggaagg aggacggcgt gcgatacctg ctgttgatct
     3481 acggcgatga gaaggccacc gcggccacgc cacccgagga gctcgaggtc agccgcaagg
     3541 cgtggatgga ttacgacggc tggctccggg agcagggctg gaggcgcgcg ggcgatgccc
     3601 tccaggagac gagcaccgcg acgacggttc gcgagaaggg cggcacgatc gtcacgaccg
     3661 atgggccgtt cgccgagacc aaggaacaac tcggtggcta ctacctgatc gagtgcgagg
     3721 acctcgacga cgcgatccag gcagcctcgc ggatgccgac catggggcga ggatcggtcg
     3781 agatccgtcc ggtccgggat gtgggcatga ggaacgagcc atcgtaggca cggacgctcc
     3841 ccaggtggtg gaccgcctgt tccggcagga gggagggcgg gcggtcgcca ccctgatccg
     3901 cgtcctcggc gacttcgatc tggccgagga cgcggtccag gaggcgttcg cgctggccct
     3961 cgagacctgg ccggcgcggg ggacgcccga gaagcccgga gcctggatca cgaggaccgc
     4021 caagaaccgc gcgatcgacc gcctccgccg ggagcggcgc ctccgcgaga agaccgagac
     4081 gctccgtcag ctcgccgagc tcgagggcct cgacgaggac atcgacatga tggggatccc
     4141 cgacgaccgg ctgcgactga tcttcacctg ctgccacccg gcgctctcga tggaggggcg
     4201 cgtggcgctg accctccgga cgctcggggg cctcaccacg gccgagatcg cccgtgcctt
     4261 cctggtcgcg gagcccacga tggcccaacg cctggttcga gcgaagcgga agatccggga
     4321 cgccgggatc ccctaccggg tgccgtcccc ggagctgctc cccgagcgcc ttcccaccgt
     4381 gctggccgtc ctctacctga tcttcaacga gggctacgcg tccacgtcgg ggccgctcgt
     4441 ccgctcggag ctgtgtgggg aggccatccg actggcacgg ctgacggacg agctcttgcc
     4501 cggccagccc gaggtgctcg gatcgatcgc gctgatgctc ctgcaccact cgcggcgcga
     4561 cgcccgaacg ggcgcggacg gccagatcgt gctgctccag gaccaggatc ggtcccgatg
     4621 ggatcactcg atgatcgacg agggcctcgc ggtcctcgac accgcactcg gcctcggcgg
     4681 gcccggtccc taccagcttc aagcggccat cgcggccctc cacgcccgcg cgccccgacc
     4741 ggaggacacc gactggcccc agatcgccgc gctctacgga ggactgatcc agatggtccc
     4801 gtccccggtg gtcgagctga accgggcggt cgcggtcgcc atggcggacg gcccgcagcg
     4861 gggcctggac ctcctggagc cgctcggtcc ggagctcgat gcctaccacc tgttccacgc
     4921 ggcccgggcc gacctcctgc gtcggctgga tcgccccggg gaagccgccc gggcctaccg
     4981 gcgggccagg gagctcgcct ccaacccggc cgagcggtcg ttcctggacc atcggcttcg
     5041 agagctcacc ggtcggcccg gcgcatgaca ccggcgcgcg aggaacactt ccgacgcgga
     5101 tccggaggcc gggccttccc cgccggcctt cagtcgatcg gcacctgagg gagcccctgg
     5161 agcgcggcct cctcatcgcc gggatcgaac tccacttcgg gaaggcgccc gtgcaggtag
     5221 tcgtcgtagg cggacatgtc gaagtgcccg tgccccgata gcccgagcag gatcacccgc
     5281 tcccgccccg cctcccgcgc cgcgatcgcc tcgttcacgg cgccgcggat cgcgtgggcg
     5341 gactcgggcg ccgggacgat gccctgcgtc tgggcgaaga gcaccgccgc ctcgaagacc
     5401 tcgttctggg cgtaggccgc ggcctccatg tagccctcac gcaccaggag cgacagcgac
     5461 ggcgagtcgc cgtggtagcg aagacccccg gcgtggaccg gagcagggac gaacccatgc
     5521 ccgagcgtgt acatcggcag cagcggcgtc agcccggccg tgtcgccgaa gtcgtaggcg
     5581 aaccgcccct tggtcagcgt gggacaggcg gcgggctcgg cggccaggaa ccgcatgtcc
     5641 gacctctcgc cccgcagctt gtcacccatg aacgggtacg cgagaccacc gtagttggag
     5701 cctcccccca cgcaccccac cacgacgtcg ggctgctccc cggtcatctc catctgcctc
     5761 cgtgcctcca ggcccaccac cgtctgatgg aggaggacgt ggttcaggca ggagccgatc
     5821 gagtagttcg tatccgggtt ggtggccgcc acctcgatcg cctcgctgat cgcgatcccg
     5881 agcgagcccg tggagtccgg gtggtcctcc aggatcttct tgccggactc ggtggtgctc
     5941 gagggagacg gtcggacctc ggccccgaag gtctccatga agatccgtcg atagggcttc
     6001 tgatcgaacg acgccttcac catgaagacc aggcactgca ggtcgaagaa ccgacaagcc
     6061 atggacaggg acgaacccca ctgaccggcg cccgtctcgg tggagatccg ccggatcccg
     6121 gcctgcttgt tgtagtaggc ctgcgccacc gcggtgttcg gcttgtgcga ccccgccggc
     6181 gagacaccct cgtacttgaa gtagatgtgc gccggcgtgt cgagggcctg ctccagccgg
     6241 accgcccgat gcagcggcgt cggcctccac agccggtaga cgtccagcac ggcccccgga
     6301 gcatcgatcc agggatcggt ggacacctcc tgcaggatca gcggctcggg gaacagcggg
     6361 gccaggaggt cgggcgtcac cggctgcttc gtctccgggt ggagcggggg caacaagccg
     6421 atgccggccc cggccatctc cgggaggatg ttgtaccagg ccggcggaac ctcctcgggc
     6481 gacaggtcga acctcgtccg ctcgatgcgc tcggccatcg gatcctcctc ggggaacgtg
     6541 tggtcgccga gcctacccga gggcgatgcg cccccgccac ccaggacccg agccggtcag
     6601 cgccggtgcg cgcgggtcac gtgccagcgg cggggatgga tcagcgcatc cgccgcgggc
     6661 ggcccccagg tcccgggctc gtaccgctgg acgggcgagg ggtcggtcag gatcgggtcc
     6721 aggatggccc aggagcgctc gacgtcgtcc tggcggagga agagggtccg atcgccctcc
     6781 atggcctcga gcaggagcag ctcgtaggca tcggcgatcc gcccaccgaa ctcctcctcg
     6841 tagtcgaaat cgatcgtggc ccggccgaga tcgagcccgg gccccggggt cttggcgttc
     6901 accgccatgg tcacgccctc gttgggctgg atgcggatcg tcagatggtc gcggggcggg
     6961 atgtcgacct cggccccccg gaacacgttg taggggacct tgcggaaggt gagcgtgacc
     7021 tcgctgcact tcgccgcgag ctccttgccc gtgcgaaggt acaccgggac gccggaccac
     7081 cgccagttgt ccacctccag ctccagggcg gcgaaggtct cgacggtcga gtcgtcggcg
     7141 acgtccggct ccccgcggta cccgcggtac tgacctcgga ccaccttggc cgggtcgcag
     7201 gccatcgtgg aacgcagcac cttcaccgtc tcgtcgcgta gccgatctgg ctcgaaggag
     7261 accggcggct ccatggtcac gaaggtcatc acctggaaga ggtgggtggc gatcatgtcg
     7321 cggatcgccc cggtctcctc gtagaaggcg ccgcggccct cgatcccgat gtcctccgcg
     7381 accgtgatct ggatgtggtc gatgtaccgg cggttccaca ccggctcgaa cagtccgttc
     7441 gagaaccgga gcgccagcac gttctgcaca gtctccttgc ccaggtagtg gtcgatgcgg
     7501 aagacctggg actcgtcgaa gacctcgtgg atcgcggcgt tcagctcccg agccgagcgc
     7561 aggtcccgac cgaacggctt ctcgaacacg atcttcgcgt cgcggtgcat cccagtctcg
     7621 gcgacccgtc gcacgacgtc cgggtacgca accggagggg tcgccgcgta gaagaaccgg
     7681 cggccctcgg tcccctgctc gcggtcgatg ccctcgaggt gaccgatcac gtggttcatc
     7741 gatcccggat cggagaattc gcccggcagg taggagaggt gtgccgcgaa gtcgcgccac
     7801 acctcgccgt ggggcgcatg ctctccgtac tccttcacgc cgtcgtgggc gatggcgcgg
     7861 aagtcgtcgt cggacagcgt cgtgcgcgag tacccgacga ttgcgaaccc cttcggcagc
     7921 agcccctgga ggaacagcga gtagaaggcc gggagcagct tcctcttggt gaggtccccg
     7981 gaagcgccga agatcaccac ggcctggggt gggaggttcg cgcccatcac gcaccaccgg
     8041 tcgcctgggg caggatctcg atccagccgt cgaccgatcg caccgggtag atcgcgatgg
     8101 ggtcgggggc tgggccgtcg accggctcgc cggagcgcag gtcgaacgcg ctcccgtaga
     8161 tcgg
//
LOCUS       DASQQQ010000338         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_23861, whole genome shotgun sequence.
ACCESSION   DASQQQ010000338 DASQQQ010000000
VERSION     DASQQQ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23861"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VE646_12680"
     CDS             complement(<1..303)
                     /locus_tag="VE646_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80878.1"
                     /translation="MGRPHLVADGQLDRGPLVGRLPEGGPLPSDGAAVAVPEGHAEAQ
                     REPHAGRAGGLVPLGHGADDQGGEGLPAGEAQAARGLLHRRRAGSQVRALAQSQGHQ"
     gene            593..1801
                     /locus_tag="VE646_12685"
     CDS             593..1801
                     /locus_tag="VE646_12685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZD80879.1"
                     /translation="MSAGGARNRRVCGALGRRARRRPFRFAPPFRTAPLAAVVLLLAC
                     SGGGEDTGGDEDEGADPNLVQLDSAALAAADLRLDSVRLVRSTVLEATGTLAYDADAV
                     AMVGPKAEGRIVRVVHDLGERVGRGEALALLESQEVGDLHAELARARAAAAVARRDYE
                     RERSLYEQEVSSEKEMLEARAAYESAVADTAAATAKLRTLGALDRDGTGFQVSTPVAG
                     TVVERDAVVGQIVGADDQLFTVADLTRLWLIVDIYEKDLARVRAGLDVEIRTRAFPDT
                     VFHGEVTYLGQVVDPDSHTVKARVVADNPGGLLRPGMFVRAGIVLPEPMGTLAVPRAA
                     VQTLRDTTVVFVPEGEGRFRAVPVRTGAQVGDGLVVLAAGVPEGQPVVGAGSFFLKSE
                     LLKATFGDID"
     gene            1812..>2301
                     /locus_tag="VE646_12690"
     CDS             1812..>2301
                     /locus_tag="VE646_12690"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZD80880.1"
                     /translation="MIDRLIRAALSNRLLVVLFALGVIGGGAYSLRRLAIDAFPDVTP
                     VLAEVITLSEGLAPQEVEQLVTYPIESAMNGLPDVQRVQSLSIFGLSDVKVYFADGVD
                     IYFARQLVRAQLQAAEEQIPPDAGEPELAPISTALGQVYQYVLRGPAYDITELRTIED
                     WIV"
BASE COUNT          292 a          827 c          874 g          308 t
ORIGIN      
        1 ctggtggccc tggctctgcg ccagcgctcg gacctgggag cccgctcggc ggcggtggag
       61 gaggccacga gccgcctgcg cctcgcccgc cgggaggccc tccccgccct ggtcgtcggc
      121 cccatggccg agcgggacga ggcctccggc tcggccagcg tggggctcgc gttgggcctc
      181 agcgtgcccc tctggaaccg caacggcggc gccgtcgctc ggcagagggc cgccctccgg
      241 caggcgacct accagcggac cgcggtcgag ctggccgtcc gccacgaggt gggggcggcc
      301 caccaggccc tgctctccgc ccgggaggag gtggacgtcc tgcgcacgtc cgtggtggag
      361 cccgcccggg cgaacgggcg gctgctggag atcgcctacc ggtcgggcaa gatcgacctg
      421 ccctccctgc tcctcgtgag gaacgagctc ctggacgccg agaccgagta ccaggaggcg
      481 tggctcgccc gccaggaggc ctggatccgg ctgcaggcgg ccaccgcatc gttcaacatg
      541 gagggagccg gccccgcggg gagggagcga cgggccgcgg ggagggatcg ccatgagcgc
      601 cggcggcgcc cggaaccggc gggtgtgcgg cgctctcggg cgacgggcgc ggcggcggcc
      661 cttccggttc gctccgccct tccgcaccgc ccccctcgcc gccgtcgtcc ttctcctcgc
      721 ctgctcgggc ggcggcgagg acaccggtgg ggacgaagac gagggcgcgg acccgaacct
      781 cgtccagctc gactcggcgg ccctggccgc ggccgacctc cggctcgact cggtgcggct
      841 ggtccggagc accgtgctcg aggccacggg caccctcgcg tacgacgccg acgccgtggc
      901 catggtgggg cccaaggccg agggccgcat cgtccgcgtg gtccacgatc tgggggagcg
      961 ggtggggcga ggtgaggccc tggcgctgct ggagagccag gaggtggggg acctccacgc
     1021 cgagctggcc cgggcgcggg ccgccgcggc cgtggcacgc cgtgactacg agcgcgaacg
     1081 gagcctctac gagcaggagg tctcctccga gaaggagatg ctggaggcac gggcggcgta
     1141 cgagtccgcc gtggccgaca cggcggcggc caccgcgaag ctccgcaccc tgggcgccct
     1201 ggaccgcgac ggcaccggct tccaggtgtc caccccggtg gccggcacgg tggtggagcg
     1261 ggacgccgtc gtgggccaga tcgtgggggc cgacgaccag ctcttcacgg tcgccgatct
     1321 gacccggctg tggctcatcg tcgacatcta cgagaaggac ctggccaggg tcagggcggg
     1381 tctggacgtg gagatccgga cccgggcgtt cccggacacg gtgttccacg gcgaggtcac
     1441 ctacctgggg caggtggtcg atcccgacag ccacacggtg aaggcgcggg tggtggccga
     1501 caacccgggc ggtctcctga ggcccgggat gttcgtccgc gccggcatcg tccttcccga
     1561 gcccatggga accctcgcgg tgcccagggc ggcggtgcag acgctgaggg atacgaccgt
     1621 ggtgttcgtg ccggagggcg agggccgctt ccgggcggtg ccggtccgga ccggcgcgca
     1681 ggtgggggac ggcctcgtcg tgctggcggc cggcgttccc gaggggcagc cggtggtggg
     1741 cgccgggagc ttcttcctga agtccgagct cctgaaggcc accttcggcg acatcgactg
     1801 agggccccgc catgatcgac cggctcatcc gcgcggccct gtccaaccgc ctcctcgtcg
     1861 tcctgttcgc cctgggggtg atcggaggcg gcgcctactc gctccgccgg ctcgccatcg
     1921 acgcctttcc cgacgtgacg ccggtgctgg cggaggtgat caccctcagc gaggggctgg
     1981 ctccccagga ggtcgagcag ctcgtcacct accccatcga atcggccatg aacgggctgc
     2041 ccgacgtcca gcgggtccag tccctgtcga tcttcgggct ctccgacgtg aaggtctact
     2101 tcgccgacgg cgtggacatc tacttcgccc ggcaactcgt ccgggcccag ctccaggcgg
     2161 cggaggagca gatccccccc gacgcggggg agcccgagct ggcccccatc agcaccgccc
     2221 tgggccaggt ctaccagtac gtgctgcggg ggccggcgta cgacatcacc gagctgcgca
     2281 ccatcgagga ctggatcgtg c
//
LOCUS       DASQQQ010000339         3004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_23995, whole genome shotgun sequence.
ACCESSION   DASQQQ010000339 DASQQQ010000000
VERSION     DASQQQ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23995"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..2668
                     /locus_tag="VE646_12695"
     CDS             215..2668
                     /locus_tag="VE646_12695"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HZD80881.1"
                     /translation="MNEQPQRRLPGGAERRVPAPYSPYGGPLGGPPVGGNGGTHEPEI
                     DWRRWAHVFWRRKWWILLVTALGTASAWYFARGERASYQANAMVWFDTPERNASSPMP
                     IEVASFLSAPSWADLLQSYAVLDGVVDELRLYVQPADAADRRLFRDFELSDDARYGTY
                     RLEVEADTTCVLYDLQDRQIQVARPGEPIGESLGWRWTPPAEGLRPGHEVIFSVRRPR
                     AVADGLSRHLETEMDQGGNFIKASFIGDDPNEAALILNSVLDHFVAVAAELKKDRLAK
                     LAGTLQGQLTQASDRLRHAESELEQYRVNTITLPEDRRDQVGVPMTYADPSGGATGVV
                     PTGPGLNTTDPVFNQFFTQQMERDRLRQDKQDLQRILKRFQGTDSLDVMLLETIPSVQ
                     TASALKSALAELGRMELERTTLLYRYTPEHAEVKRATESINQLKAQTIPSLISGLIGR
                     LSERESEVEGQLDAQKSELQDIPPRAIEEARLRREVALAEQVHNNLQVRYKEAQVAEA
                     ATQPSVRVLDRAVAPPSSMGSTFRLKVMIGFVLSLGLALVGVIGYEQYVNRRLEYPEQ
                     VVGDLGLPILGIVPNLKALKAAPTAEGNGDGPEASGAIESFRALRTQLTHGLRIKYPA
                     VIAITSPSMGEGKSLVTSNLALAFAEPGRPTVLIDGDTRRGGLHRIFQVPRSPGLTDY
                     LRGEADARRIVRRTKSGGLSFVPRGPWSEEIPELMDGEGLPTLLEQLKHDYDIILVDT
                     PPLAAGVDAVLIGAHADAVLAVLRTGRTDLDVARAKIASYAQMLGVPILGAILNDVKE
                     EGPYRYYSYYSYGAYSGGR"
     gene            2672..>3004
                     /locus_tag="VE646_12700"
     CDS             2672..>3004
                     /locus_tag="VE646_12700"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZD80882.1"
                     /translation="MSPDGFVRGNGLIAGNALVTGAAGFIGSHIVDRLLDRGWYVWGI
                     DNFDAFYGRRIKESNLEEALPQSNFRLAEGDIRDDGLLAQVFREGQFDVVLHMAARAG
                     VRPSIEAPD"
BASE COUNT          469 a          978 c         1137 g          420 t
ORIGIN      
        1 gatctgttgc ggatctccca catctggagg gcggcgcacg tggatcgccg ctcccgcaac
       61 gacttaccgg cctcatcctc aggcacctca cttgctacgt tgtcccctgc tgccgcccgc
      121 gacgtgacgg cacggggccc cggaggcgga agagcgccag tttccgggtc cctgcgggat
      181 cgaaaacgca agcggtcaat cgataaggag cacgatgaac gagcagcctc agcgccggtt
      241 gccgggaggg gccgagagac gggtgccggc gccgtactcg ccctacggcg ggcccctggg
      301 cgggccgccg gtcgggggga acggcggaac gcacgagccg gagatcgact ggcgccgctg
      361 ggcccacgta ttctggcgcc gcaagtggtg gatcctgttg gtgacggcgc tgggcacggc
      421 gtcggcctgg tacttcgcgc ggggcgagcg ggcgagctac caggccaacg cgatggtctg
      481 gttcgacacc ccggagcgca acgcctcgtc cccgatgccg atcgaggtgg cgagcttcct
      541 gtccgcgccg agctgggcgg atctgctgca gagctacgcg gtgctggacg gcgtggtgga
      601 cgagctgagg ctctacgtcc agccggccga cgcggccgat cgccgtctgt tccgcgattt
      661 cgagctcagc gatgacgccc gctacgggac gtaccggctg gaggtggagg cggacacgac
      721 ctgcgtcctc tacgacctcc aggaccgaca gatccaggtg gcgcggccgg gcgagccgat
      781 cggcgagtcg ctgggctggc ggtggacgcc gccggcggag gggctgcggc ccggccacga
      841 ggtgatcttc agcgtccggc gtccccgggc ggtggcggac ggcctgagcc ggcacctgga
      901 gacggagatg gaccagggcg gcaacttcat caaggcgagc ttcatcggcg acgacccgaa
      961 cgaggcggcc ctgatcctga actcggtcct ggaccacttc gtggccgtcg ccgccgagct
     1021 gaagaaggac cggctggcga agctggcggg cacgctgcag gggcagctca cccaggcctc
     1081 ggaccggctg cgccacgccg agtcggagct ggagcagtac cgggtcaaca cgatcacgct
     1141 gcccgaggac cgccgggacc aggtcggcgt cccgatgacc tacgcggatc cgagcggcgg
     1201 cgccaccggg gtcgtcccga ccgggccggg cctcaacacg acggacccgg tgttcaacca
     1261 gttcttcacg cagcagatgg agcgggaccg gctgcgacag gacaagcagg acctgcagcg
     1321 gatcctgaag cggttccagg ggacggacag cctggacgtc atgctgctgg agacgatccc
     1381 gtcggtgcag accgcatcgg cgctgaagtc ggcgttggcg gagctgggcc ggatggagct
     1441 ggagcggacc acgctcctct accgctacac gccggagcac gccgaggtga agcgggccac
     1501 ggaatcgatc aaccagctga aggcgcagac gatcccgtcc ctgatctcgg gcctgatcgg
     1561 ccgcctctcc gagcgggagt ccgaggtcga gggccagctg gacgcccaga agtcggagct
     1621 gcaggacatc ccgccgcggg cgatcgagga ggcgcggctg cgccgggagg tggccctggc
     1681 ggagcaggtc cacaacaacc tgcaggtccg ttacaaggag gcccaggtgg cggaggccgc
     1741 cacccagccg agcgtccggg tcctggatcg cgccgtggcg ccgccgtcct ccatggggag
     1801 caccttccgg ctgaaggtga tgatcggctt cgtgctgagc ctcgggctgg ctctggtggg
     1861 ggtcatcggc tacgaacagt acgtgaaccg gcggctggag tatccggagc aggtcgtcgg
     1921 cgacctgggt ctcccgatcc tggggatcgt acccaacctg aaggcgctca aggccgcgcc
     1981 gaccgcggag gggaacgggg acgggccgga ggcgtccggg gcgatcgagt cgttccgggc
     2041 cctgcggacc cagctcaccc acggccttcg catcaagtac cccgcggtga tcgcgatcac
     2101 cagcccgtcg atgggcgaag ggaagtcgct ggtgacctcc aacctggcgc tcgccttcgc
     2161 ggagcccggc cggccgaccg tcctgatcga cggcgacacg cggcgcggcg gcctgcaccg
     2221 gatcttccag gtgccccgga gccccggtct gaccgactac ctgcggggcg aggcggatgc
     2281 caggcggatc gtgcggcgca cgaagtcggg cggcctctcc ttcgtgccgc gggggccgtg
     2341 gtccgaggag atcccggagc tgatggacgg cgagggcctg ccgacgctgc tggagcagct
     2401 gaagcatgac tacgacatca tcctggtgga cacgccgccg ctggcggcgg gcgtggacgc
     2461 ggtgctgatc ggcgcccacg cggatgcggt gctggcggtg ctgcggaccg ggcggacgga
     2521 cctggacgtg gcccgggcca agatcgcgtc ctacgcccag atgctgggcg tgccgatcct
     2581 gggggcgatt ctgaacgacg tgaaggagga gggcccgtac cggtactaca gctactacag
     2641 ctacggcgcc tactcggggg ggaggtgacc catgtcgccg gacgggttcg tgcgggggaa
     2701 cggattgatc gccgggaacg ccctggtcac gggcgcggcg gggttcatcg gctcccacat
     2761 cgtggatcgg ctgctggacc ggggctggta cgtgtggggg atcgacaact tcgacgcctt
     2821 ctacgggcgg cgcatcaagg agtcgaacct ggaggaggcg ctcccccagt cgaacttccg
     2881 gctggcggag ggggacatcc gggacgatgg tctcctggcc caggtgttcc gggaggggca
     2941 gttcgacgtc gtgctccaca tggcggcccg ggccggggtc cgtccatcga tcgaggcgcc
     3001 ggac
//
LOCUS       DASQQQ010000340         5568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_51848, whole genome shotgun sequence.
ACCESSION   DASQQQ010000340 DASQQQ010000000
VERSION     DASQQQ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51848"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /locus_tag="VE646_12705"
     CDS             <1..1304
                     /locus_tag="VE646_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80883.1"
                     /translation="TGLAVLGAAVAAVLVFPLLPDWAGDPGGSFSSVIGTMDFARLGR
                     LAPGGGPGTWVVAWFLPVSAVLAFSVVGPGYRARGWRAMAVAVGGVFLAWLSTAGYLP
                     GGLTNAPAYLVAAAGAEALVVGYGVATIGAGMTSLSFGYRQIGAGLLAATLALGFGGQ
                     TLRAIVGGWSVGPSALPSAWPVIDSATGSFRIAWIGRDDGAAFPAPGGDPQGVVHAGE
                     LSLRYALTDRRGVSALDVGRGPVGQGYEFLERALEELLAGGTSHAGALLAPLGVRFLV
                     ARAGDLPPTVERRLQAQVDLDLVPAGGLMIYRDARALPPAFLVPPSSDATIRTADLEA
                     ISSMSSLHATPLVWRASGVRVPEQEGTAVLGQQFDPGWRATSTGGTARPERAFGWAMA
                     FPDASGSSISFSRQWVRTAEIAALAVLWLAALWITRRPATR"
     gene            1301..2686
                     /locus_tag="VE646_12710"
     CDS             1301..2686
                     /locus_tag="VE646_12710"
                     /inference="COORDINATES: protein motif:HMM:NF040160.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HZD80884.1"
                     /translation="MRARGQFLSAVLLVALVTMGAVYLQDMVGPRSEVTAATVLAPSG
                     AWFCPHGGGEGWTTSLQVANPGTAPVQIRVTGIGTGKPSPPEELTVAPGTEVRVPAPA
                     GQRASSTTVEYFGGWVAAGWLTHAGGGQSGVAAEPCLPHASGRWLLPDGSAERNQDDY
                     LVVMNPFSAEAVFTVTLFTAKRPAPITPSEWTNVVLKPHRSQAFRLNPVALGEATVSA
                     LVQAKVGRVAASTLGLSDPGGVRSSVGVPGTPPTSSILPGAFDQGATDLVVMDPTERP
                     AALQATVLGREGPQAIANLEDSSTNASSAVTYPVTTEGPSALVVPSPPGVAVARRTSG
                     VASDQGSTTGAGTPAAAWVVLPAVAGSPSHPGMVLVNPADAPVHVTLSYLPADEGTVP
                     APVTLQVPPRSSVAAPKHFLEAKPMSAVLAAASDGTFVPAAASYSLGIRGVAGYAVSL
                     GVAIPPAWVPR"
     gene            2825..3889
                     /locus_tag="VE646_12715"
     CDS             2825..3889
                     /locus_tag="VE646_12715"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZD80885.1"
                     /translation="MARGRHTRRAIGRGWVVLVTTLALLVALGGGSAYAAYHYDRSSS
                     SHIMPGVSVAGVDVSGMTREQAVRAVEQRADLTLEATLTVRALGHRWRVTPASLGTAA
                     DVQGAVERAFQVADTMSLVSRLYHRIADRSMTLDIPLGYTYDRSKVEAFVQQVSDRVR
                     EPAVNADFSLVDGGLSMHGAKTGLELKGKLATDRVVRALQERRDAVRIPVRTVHPEVT
                     VADLGKTIVVDLSENHLYLYEGFKVVEDYPVATAAPGYTTPVGTWEVVNKAENPTWYN
                     PAPDGWGAGEPLVIPPGPGNPLGTRALYLNAPGIRIHGTYSSSSIGTHASHGCIRMYI
                     SDAEQLYPLVPIGTKVIIKP"
     gene            3931..4140
                     /locus_tag="VE646_12720"
     CDS             3931..4140
                     /locus_tag="VE646_12720"
                     /inference="COORDINATES: protein motif:HMM:NF023430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HZD80886.1"
                     /translation="MRSCAKLRCRCEAVSTVGLSYEAKVVVIGDLSPEPNPNLVDLCS
                     EHVERLIPPMGWRIRDERSPLAVTG"
     gene            4307..4498
                     /locus_tag="VE646_12725"
     CDS             4307..4498
                     /locus_tag="VE646_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80887.1"
                     /translation="MDGITSGTCPVCHQGELITITMSVARSDLAFTTCHLCEAKWWIR
                     DGEPVPLQSVIGSVLSAKD"
     gene            4525..>5568
                     /gene="manB"
                     /locus_tag="VE646_12730"
     CDS             4525..>5568
                     /gene="manB"
                     /locus_tag="VE646_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142783.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HZD80888.1"
                     /translation="MIVTSRPDLGRIFKAYDVRGVVPDDLDQDVARRIGSAFAEWASA
                     PRIAVGYDCRLSSPSLAEAFREGAVTRGVDVVDLGLASTDLLYFASGSLGVPGTMLTA
                     SHNPPQWNGLKFCLAGAKPVGRDTGLADIRALAERDRYSPATSTGTVEQRSLIDEYAQ
                     HVLSFVDGAEMRPLTVVADTANGMGGLVVPAVMARLPVTLIHLYPDLDGTFPNHPADP
                     IDPENQRDLKRAVLEHHADIGLAFDGDADRVFLVDERAEGVSGSEVTALVARSMLERH
                     PGASIVYNLICSWAVPEVIRENGGTPVRTRVGHSFIKQVMADTGAVFGGEHSGHYYFR
                     ENYRADSGLIAAVV"
BASE COUNT          737 a         1941 c         2025 g          865 t
ORIGIN      
        1 gcaccgggct ggcggtgctg ggcgcggcag ttgccgcggt cctggtcttc cccttgcttc
       61 ccgattgggc cggcgacccc gggggttcct tctcttcggt catcggcacg atggacttcg
      121 cccgcctggg acgtctggct cccggcggcg ggccggggac ctgggtggtg gcatggttcc
      181 tacccgtctc cgcggtgctc gccttcagcg tggtgggccc tgggtaccgg gcccgcgggt
      241 ggcgcgccat ggcggtggcc gtgggcgggg tgttcctggc gtggctctcg acggccggtt
      301 acctgcctgg cgggctcacg aacgcccccg cgtacctggt ggcggcggcc ggggccgagg
      361 cgctggtggt cggctacggc gtggccacca tcggtgccgg gatgacctcg ctgtccttcg
      421 gctaccggca gatcggtgcg ggcttgctgg cggccacgct cgccctgggt ttcggggggc
      481 agacgctccg agcgatcgtg gggggctggt cggttggtcc ctccgccctg ccgtccgcct
      541 ggccggtgat cgacagtgcc accgggagtt tccgcatcgc gtggatcggc cgagacgacg
      601 gggcggcgtt ccccgcgcct gggggcgacc cccagggggt ggtgcacgca ggggagctct
      661 ccctgcgata cgcgctcacc gaccggcgag gcgtctccgc gctcgacgtc ggccgcgggc
      721 cggtcggcca ggggtacgag ttcctggagc gggccctcga ggagctcctg gccggcggca
      781 ccagccacgc cggagccctg ctcgccccgc tcggggtccg gttcctcgtc gcccgggccg
      841 gcgatcttcc tcccacggtc gaacgacgac tgcaggcgca ggtcgacctg gacctggtgc
      901 ccgcgggcgg cctgatgatc taccgggacg cgcgggccct gcctcctgcg ttcctggtcc
      961 caccgagctc ggatgccacg atccggacgg cggacctgga ggccatctcc tccatgtcgt
     1021 ctctccacgc gaccccgctc gtgtggcggg cgagcggtgt gcgcgtgccg gagcaggagg
     1081 ggacggccgt cctgggccag cagttcgatc ccggttggcg cgccacctcg acgggcggga
     1141 ccgcccgtcc cgagcgcgcc ttcggatggg ccatggcgtt cccggatgct tcgggctcct
     1201 ccatctcgtt ctcgcgtcag tgggtgcgga cggccgagat cgcggcgctg gccgtgctgt
     1261 ggttggcggc cctatggatc acgcggaggc ccgcgacgcg atgagggcgc gaggacagtt
     1321 cctctcggcc gtgctgctgg tggccctggt gacgatgggc gccgtctacc tgcaggacat
     1381 ggtcggtcca cgttcggagg tgacggccgc caccgtgctg gcgccgtcgg gggcctggtt
     1441 ctgccctcac gggggcggtg agggctggac cacgtcgctg caggtggcca acccggggac
     1501 cgcaccggtc cagatccggg tgaccggtat cggcaccggc aagccctcgc ccccagagga
     1561 gctcaccgtg gctcccggca ccgaggtgcg cgtccccgct cccgccggcc agcgcgcctc
     1621 gagcaccacg gtcgagtact tcggcggctg ggtggccgcg ggctggctga cccacgccgg
     1681 cggcgggcag agcggtgtgg cggccgagcc gtgcctgccc cacgcatcgg gccgctggct
     1741 gctccccgac gggagtgcgg aacgcaacca ggacgactac ctggtggtga tgaacccctt
     1801 ctccgcggaa gcggtcttca cggtcacgtt gttcacggcc aagcggccgg cccccatcac
     1861 acccagcgag tggacgaacg tggtgctgaa gccgcatcgg agccaggcct tccgcctgaa
     1921 ccccgtggcc ctgggcgagg ccacggtctc ggccctcgtg caggccaagg tggggcgggt
     1981 ggcggcctcc acgctgggcc tgtcggaccc gggcggggtc cggagcagcg tgggcgtccc
     2041 cgggacgcct cccacctcgt cgatcctgcc gggggccttc gaccaggggg ccaccgacct
     2101 ggtggtgatg gatcccaccg agcgacccgc cgcgctccag gcgaccgtac tgggcaggga
     2161 gggcccgcag gccatcgcca acctcgagga ctcgagcacg aacgcctcct ccgccgtcac
     2221 ctatccggtc acgaccgagg gcccgagcgc cctagtggtg ccctcgccac ccggggtggc
     2281 cgtagcccgc aggacgtcgg gcgtggcctc ggatcagggc tcgaccacgg gcgcgggcac
     2341 gcccgccgca gcctgggtag tgctgccggc cgtggccggc tctccctcgc atccgggcat
     2401 ggtcctggtc aacccggcgg acgccccggt gcacgtgacg ctctcctacc tgccggccga
     2461 tgaaggaacc gtcccggcgc cggtgaccct gcaggtccct ccgaggagct cggtcgccgc
     2521 cccgaagcac ttcttggagg ccaagccgat gtcggccgtc ctcgccgcgg catcggacgg
     2581 aacgttcgtg cctgcggccg cgtcctactc cctggggatc cgcggagtgg ccgggtacgc
     2641 cgtgtccctg ggcgttgcca tcccgcccgc gtgggttccg agatgaaccg tacgcatgag
     2701 ctgcacatga tcgatgtgca gaggatgtgg cggtgaccca tccgggtaca gaccgtatga
     2761 tgtgcccttc ggcaacccgg gggcgggtgc gccgcaaggg acgagggatc tgacggggag
     2821 cgggatggcg cggggaaggc acaccaggcg agcgatcggc cgcggttggg tcgtgctggt
     2881 gacgaccctc gcgctcctcg tggcgctggg cgggggttcc gcctacgccg cctaccatta
     2941 cgaccgatcc agctcgtccc acatcatgcc gggggtcagc gtggccggcg tggatgtgag
     3001 cgggatgacc cgggagcaag cggtgcgcgc cgtggagcaa cgggccgatc tcaccctgga
     3061 ggccaccctc accgtgcggg ctctgggcca ccgctggagg gtcacccccg cctcgctggg
     3121 gaccgccgcc gacgtccagg gagcggtgga acgcgccttc caggtcgccg acacgatgtc
     3181 gctggtctca cgcctgtacc accggatcgc cgatcggtcg atgaccctcg acatcccgct
     3241 cgggtacacc tacgaccgat ccaaggtcga ggcgttcgtc cagcaggtct ccgaccgggt
     3301 ccgggagccc gccgtgaacg ccgacttctc gctggtcgac ggcgggctgt ccatgcacgg
     3361 ggccaagacc ggactggagc tgaagggcaa gctggccacg gaccgggtgg tgcgggccct
     3421 gcaggagcgg cgggacgcgg tgcggatccc ggtccgaacg gtccaccccg aggtgacggt
     3481 cgccgatctg ggcaagacca tcgtcgtgga cctgtccgag aaccatctgt acctgtacga
     3541 gggattcaag gtggtcgagg actacccggt ggccaccgcc gcgccgggct acaccacccc
     3601 ggtggggacc tgggaggtcg tgaacaaggc cgagaacccc acctggtaca accctgcgcc
     3661 cgacgggtgg ggggccggcg aaccgctggt gatcccaccg ggccccggga acccgctggg
     3721 gacgcgcgcc ctgtacctga acgcgccggg gatccggatc cacggcacat actcctccag
     3781 ctcgatcggc acccacgcat cgcacggctg tatccggatg tacatctccg atgccgagca
     3841 gttgtacccg ctggtgccca tcggcaccaa ggtcatcatc aagccctgaa cctgcttgag
     3901 gatcacacgc atgtcatcta ccatccgcct gtgcgatcgt gcgcgaagct gcggtgccgg
     3961 tgtgaggcgg tctccacggt ggggctgtcc tatgaggcca aggtcgtcgt catcggcgac
     4021 ctgtctcccg agccgaaccc gaacctggtc gacctctgtt ccgagcacgt ggaacggctg
     4081 atcccgccga tggggtggcg gatccgcgac gagcgctcgc ccctcgccgt gaccggctag
     4141 gtccggccat gggccgttcg gcctcccccg agaggtgacg ggtgtgccgc cgatcgggga
     4201 ggctacgtga cccggtggcc gagcatccgc cccgcccgga tcggccggag gaaatggggc
     4261 caccgtgcta tgggcgcggg agcgtcgatg cggcaccatc acacgtatgg acggcatcac
     4321 cagcgggacc tgccccgttt gtcatcaggg cgagctgatc accatcacga tgtccgttgc
     4381 ccgaagcgac ctggccttca ccacgtgcca tctgtgcgag gccaagtggt ggatccgcga
     4441 cggcgagccc gtccccctgc agtcggtcat cggctccgta ctctccgcga aggactgatc
     4501 gatccgccgc tccagtagga tgcgatgatc gtgacatcgc gtccggatct gggtcggatc
     4561 ttcaaggcgt acgacgttcg aggcgtggtg cccgacgatc tggaccagga cgtcgcacgc
     4621 cggatcggct cggcgttcgc ggagtgggcc tccgctccca ggatcgccgt cgggtacgac
     4681 tgccggctgt cctcgccgtc gctggccgag gccttccggg agggcgccgt aaccaggggc
     4741 gtggacgtgg tggacctggg cctcgcctcc accgacctgc tgtacttcgc gtccgggtcg
     4801 ttgggggtgc ccgggaccat gctgacggcc agccacaacc cgccgcagtg gaacgggctc
     4861 aagttctgcc tggccggggc caagcccgtg ggccgggaca ccggactggc cgacatccgg
     4921 gccctggcgg agcgcgaccg gtactctccg gccacctcca cgggaacggt cgagcagcgg
     4981 tcgctgatcg acgagtacgc gcagcacgtc ctgtcgttcg tggatggcgc ggagatgcgg
     5041 ccgctcacgg tggtggccga caccgcgaac ggcatgggcg ggctggtggt gccggccgtc
     5101 atggctcggc tgcccgtcac cctgatccac ctctacccgg acctggacgg cacgttcccc
     5161 aatcacccgg cggatccgat cgacccggag aaccagcgcg acctgaagcg ggccgtcctg
     5221 gagcatcacg ccgatatcgg cctggccttc gatggggacg ccgaccgcgt gttcttggtg
     5281 gacgagcggg cggagggggt gagcggctcc gaggtgacgg cgctggtcgc ccggtccatg
     5341 ctggagcgtc atccgggggc ctcgatcgtc tacaacctga tctgcagctg ggccgtgccc
     5401 gaggtgatcc gtgagaacgg agggaccccg gtccgcacca gggtgggaca ctcgttcatc
     5461 aagcaggtca tggcggacac gggggccgtg ttcggcgggg agcactcggg tcactactac
     5521 ttccgcgaga actaccgggc cgactcgggg ctgatcgccg ccgtggtc
//
LOCUS       DASQQQ010000341         3005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_24087, whole genome shotgun sequence.
ACCESSION   DASQQQ010000341 DASQQQ010000000
VERSION     DASQQQ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24087"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..787)
                     /locus_tag="VE646_12735"
     CDS             complement(212..787)
                     /locus_tag="VE646_12735"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZD80889.1"
                     /translation="MISAIVLAAGTSSRFGRTKQVVEVGGKPLAQHAVDAAHASGVDE
                     IVVVLGHDATAVRERLHLPSTARTVVNAAYAQGQATSLAAGLAEADPHSEAAVVLMAD
                     QPGIEARHVRALVEAFTEEPTPIVRLRFRDGPGPALLARRTWPAARALRGDAGARQLI
                     AGHPELVRELPLDEDAPPDVDTPGDLAGDAG"
     gene            complement(784..1485)
                     /locus_tag="VE646_12740"
     CDS             complement(784..1485)
                     /locus_tag="VE646_12740"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZD80890.1"
                     /translation="MLIENEFDVPAPLDRAWDHLLDVERVAPCAPGAELTEVVDDHTW
                     KGRMRMKLGPVSLSFAGTVTMDERDDQAHRVVLVAKGQETKGKGAASASVTSWLEDRG
                     AETRVKMRADITLSGTVAQLSRGLLPDVSARLTHRFAECLRTTLSQAEPSTGEAASAA
                     GGAGDANTVAPPEEGARPLRSGVGDGMASGATAPPQPPHARPQAPPIGGIRLGLWAIW
                     RAIVRVVRRLFGGGR"
     gene            1597..2373
                     /locus_tag="VE646_12745"
     CDS             1597..2373
                     /locus_tag="VE646_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012724245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZD80891.1"
                     /translation="MRGLAGRVALVTGAGSPSGIGAACARVLGREGAMLAIASTTERI
                     REREDELRADRFPVAGFMADLTVFEHARTLVGEVLERYGRIDVLVNNAGMVQTGVEDA
                     SKRLVELSEQDWDRAIALNLTTAFNVTRHVLPGMLERGYGRIVNVSSVTGPLVAIAES
                     VGYGAAKAGMDGLTRGLALEVGRHGVTVNSVAPGWIRTGSSTPEELVAGEHTPVGRPG
                     TPEEVAEVVGFLASEGASYVTGRSIVVDGGNTIQEVKGPA"
     gene            complement(2381..>3005)
                     /locus_tag="VE646_12750"
     CDS             complement(2381..>3005)
                     /locus_tag="VE646_12750"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HZD80892.1"
                     /translation="LLERERSEIRDLAVGEGTARAFVEVLEPPLRVLICGAGHDAAPL
                     VRAAAGLGWSPVVADDRGEFLNRERFPEASEFVALERPEDVAKAAPIDRATFVVVMTH
                     NFLRDKDYARSLIDTPARYVAMLGPNARTERLLTELREEGVEIPQHFLERVHGPAGLD
                     LGAEGPEEIAAAIVSEIIAVKRGRGGGFLKHRPGPIHERQPSARRQA"
BASE COUNT          395 a         1071 c         1119 g          420 t
ORIGIN      
        1 atgcgcagca cgccgcaccg ggagaacatc ctccggacga gcttcgacca cgccgctacc
       61 ggcgtcgtgc ggtcaggcgg gaaggtctgg gtgaccgtca tcttctacgg ctagcccctc
      121 ccgacctagc tgtaggcaca cgcccgggcc accaagggcc cgggcgtcgt gcgtccgggc
      181 tcggatggat cggaggagga ccggcgtccc ctcagccggc gtcgcctgcc agatctcccg
      241 gcgtgtccac gtcgggcggg gcgtcctcgt ccagggggag ctcgcggacc agctcggggt
      301 gaccggcgat cagctgcctg gccccggcat cgcctcggag cgcccgggcg gcaggccagg
      361 tccggcgggc cagcagcgcg ggccccggcc cgtctcggaa ccggagccga acgatcggcg
      421 tcggctcctc ggtgaaggcc tcgaccagcg ctcggacgtg acgggcctcg atgcccggct
      481 ggtcggccat cagcaccacc gctgcctcgc tgtgggggtc cgcctcggcc aggccggccg
      541 ccaaggacgt ggcctgtccc tgcgcgtacg cggcgttcac caccgtacgg gcggtggagg
      601 gcaggtggag ccgctcccgc accgccgtcg cgtcgtggcc gaggaccacc acgatctcgt
      661 ccacgccgga cgcgtgggcg gcgtccaccg cgtgctgggc cagcggcttg ccgcccacct
      721 cgaccacctg cttcgtgcgg ccgaaccgcg aggacgtgcc ggccgccagc acgatggccg
      781 agatcaccga ccgccgccga acaggcgccg aacgacgcgg acgatcgccc gccagatcgc
      841 ccacaggccc aggcggatgc ctccgatcgg tggtgcttgc ggtcgtgcgt gcggtggttg
      901 gggcggggcc gttgctccgc ttgccatgcc atcgcctacc ccgctccgca gcggcctcgc
      961 cccttcctca ggcggggcca cggtgttcgc gtcccctgcc cctccagccg cgcttgccgc
     1021 ttccccggtg gacggttcgg cctgggaaag ggtggtgcgg aggcattcgg cgaagcggtg
     1081 ggtcaggcgg gcggagacgt cggggaggag gcctcgcgag agctgggcga ccgtgccgga
     1141 gagggtgatg tcggcccgca tcttcaccct ggtctcggcg ccccggtcct ccagccagga
     1201 ggtcacggag gcggacgccg cccccttccc cttcgtctcc tggcccttgg cgaccagcac
     1261 cacgcggtgc gcctggtcgt cgcgctcgtc catcgtcacg gtgccggcga acgagagtga
     1321 gaccgggccg agcttcatcc gcatccggcc cttccacgtg tggtcgtcca ccacctcggt
     1381 gagctccgcc ccgggggcgc agggagccac ccgctccacg tcgagcaggt ggtcccaggc
     1441 ccggtccagg ggggcgggga cgtcgaactc gttctcgatc agcaccgcat ctccgttccg
     1501 ctcgctcggc cccgggagcg tatcgggagg gggggagggg tgccaccgct tcggataggg
     1561 ttccgccggc tggtctccat cgggaagggg ggcgaggtgc gcggcttggc agggcgggtg
     1621 gcactggtga cgggggcggg aagccccagc ggcatcggtg cggcatgcgc gcgggtcctg
     1681 gggagggagg gcgcgatgct cgcgatcgcc tcgaccaccg agcgcatccg cgagcgcgaa
     1741 gacgagctgc gggccgaccg gttcccggtt gcgggcttca tggccgacct caccgtcttc
     1801 gagcacgcgc gcacgttggt gggcgaggtc ctggagcggt acggacgcat cgacgtgctg
     1861 gtgaacaacg ccggcatggt gcagacgggg gtagaggacg cctcgaagcg gttggtggag
     1921 ctctccgagc aggactggga tcgggccatc gcgctcaacc tcaccacggc cttcaacgtc
     1981 acccggcacg tcctgccggg catgctcgag cgtggctacg ggaggatcgt gaacgtctcc
     2041 tcggtgaccg ggcccctggt ggcgatcgcc gagagcgtgg gctacggagc cgccaaggcc
     2101 gggatggacg gcctcacccg aggactggcc ctcgaggtcg gtcggcacgg ggtcaccgtg
     2161 aactcggtcg caccggggtg gatccggacg ggttcgtcca cgcccgagga gctggtggcc
     2221 ggcgagcaca ccccggtggg ccgtcccggc acccccgagg aggtggccga ggtggtgggc
     2281 ttcctggcct ccgagggcgc gagctacgtg accggccggt cgatcgtggt cgacggcggc
     2341 aacacgatcc aggaggtcaa gggcccggcc tgatcgccgg ctacgcctgc cggcgcgcgg
     2401 aaggctgccg ctcgtggatc ggacccgggc ggtgcttgag gaagccgcct ccgcggccgc
     2461 gcttgaccgc gatgatctcg gacacgatgg ccgcggcgat ctcctcgggc ccctcggcgc
     2521 cgagatccag gccggccggc ccatggaccc gttcgaggaa gtgctggggg atctccacgc
     2581 cttcctcccg gagctccgtc agcagccgct cggtgcgggc gttcggaccg agcatcgcca
     2641 cgtagcgggc cggtgtgtcg atcagcgagc gagcgtagtc cttgtcgcgc aggaagttgt
     2701 gggtcatcac cacgacgaag gtggcgcgat cgatcggcgc cgccttggcc acgtcctcgg
     2761 gtcgctccag ggcaacgaac tccgacgcct cggggaagcg ctcgcggttc aggaactccc
     2821 cacgatcgtc ggccaccacg gggctccacc ccagcccggc ggcggcgcgg accaggggcg
     2881 ccgcgtcgtg gcccgctccg cagatcaaca cgcggagcgg cggctccagc acctcgacga
     2941 aggcgcgcgc cgtgccctcg cccaccgcca gatcgcggat ctcgctccgt tcgcgctcga
     3001 gcagg
//
LOCUS       DASQQQ010000342         2852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_51970, whole genome shotgun sequence.
ACCESSION   DASQQQ010000342 DASQQQ010000000
VERSION     DASQQQ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51970"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            570..1748
                     /locus_tag="VE646_12755"
     CDS             570..1748
                     /locus_tag="VE646_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017717332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HZD80893.1"
                     /translation="MQHAETFIGRLVAALSRDTAAFREAWPGRAGALLAALARGGPAV
                     VSRDDRSTSPAPDATISSDKTVPLRIRTSRGLHRALGRGATLRGADLYGATLPGVELH
                     RVNLEGADLHGADLHRATLREATLRGATLQGATLGGANLRRANLRGATLGEADLRGAG
                     LGWADLEGADLSGATLHQASLHGANLRGATLRGATLGEADLEGANLEGANLEGAELSG
                     ATLHQATLGGANLRGAGLREANLRADLGGAGLGWADLEGADLSGASLHRATLREANLR
                     WANLRGADLREANLRGANLAAANLAAANLHEATLRGANLAGASLGGADLEGADLSGAS
                     LHEASLQGANLRRARWTEETKWPASVELSIRVRSEEVGLGVFRVRGGTERVPVPHQTC
                     "
     gene            2217..>2852
                     /locus_tag="VE646_12760"
     CDS             2217..>2852
                     /locus_tag="VE646_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD80894.1"
                     /translation="MRLLVVEDETRLALLLKRGLEEQGYAVDVTGDGAEALWYATEVD
                     YDAIVLDIMLPSMDGLEVTRRLRRGGRWVPVLLLTARDAVDARVVGLDAGADDYLVKP
                     FSFAELAARVRALVRRGRVERPAVLEVGDLRLDPARRQAWRGQVELDLSPTEFALLEL
                     FLSHPDEALTRTRILEHVWDFAYDPASNVVDQYVAYLRRKIDRPFGRADLET"
BASE COUNT          413 a         1061 c         1003 g          375 t
ORIGIN      
        1 ggagcgccgc catcgtctcg gcccgctcgt tcagcggatc gcccacgccg cctcctcacc
       61 gtccacgtcg gctcagtgca cggacgatgc ccgccgcgat ggtgatggcg gagtgggccg
      121 cgcctgcaca gggcaccggc cagccgcatc gtcggccgta gtgcaacggt cgcgaaaact
      181 gctatacacg gccacctaga ccgatgtata actaacgacg actttggccg gaacgctgac
      241 gaagcgctcg ggggggtggc caccgtcatg acagcgcggg acgacgcggt acggggactg
      301 ttcggcggtt tccccatcga cgccttggag tccgctggaa ctgaccggcg cgttgccggc
      361 tggtcgtggc atcggctcgg cgagcccgca ccacccccgc ggcgggctgc ccatgcacca
      421 ccgcctcacc gcccgagggc caagccccca ccactccggg ggactccggg ggccccgggc
      481 ccccgcagcc agcccctcgc cgatgaccgc attgaaccct gacgccgcga tgccagcgca
      541 gaccgaacac gcacaaggcg gggattgcga tgcagcatgc cgagacgttc atcggccgcc
      601 tcgtggcggc gttgtcgcgc gataccgccg cgttccgaga ggcgtggccc ggtagagccg
      661 gcgcgctgct cgccgccctg gcgcgcggcg gccccgcggt cgtttcgcgc gacgaccggt
      721 ccacctcacc ggccccagac gccaccatca gcagcgacaa gaccgtcccg ctacgtatcc
      781 gcacctcaag aggtctccac agggcccttg gccgcggggc caccctccgc ggagccgacc
      841 tttacggggc caccctccct ggggtcgagc tccaccgggt caacctggag ggggccgacc
      901 tgcacggagc cgatctccac cgcgccaccc tccgcgaggc caccctccgc ggcgccacgc
      961 tccagggagc caccctcggc ggggccaacc tgcgcagggc caacctgcgc ggcgccacgc
     1021 tcggcgaggc cgacctccgc ggggccggcc tgggctgggc ggacctggag ggcgccgacc
     1081 tcagcggagc caccctccac caggccagcc tccatggcgc caacctccgt ggggccaccc
     1141 tccgcggggc caccctcggt gaggccgacc tggagggcgc caacctcgag ggcgccaacc
     1201 tggagggcgc cgagctcagc ggagccaccc tgcaccaggc caccctcggc ggggccaacc
     1261 tccgcggggc cggcctccgc gaggccaacc tgcgcgcgga cctcggcggg gccggcctgg
     1321 gctgggcgga cctggagggc gccgacctca gcggagcctc cctccaccgt gccaccctcc
     1381 gcgaggccaa cctgcgctgg gccaacctgc gcggggccga tctccgggaa gccaacctcc
     1441 gcggggccaa cctcgccgcc gccaacctcg ccgccgccaa cctccacgag gccaccctcc
     1501 gcggggccaa cctcgccgga gccagcctcg gcggggcgga cctggagggc gccgacctca
     1561 gcggagcctc cctccacgaa gcctccctcc agggggccaa cctccgaagg gcccgatgga
     1621 cagaggagac gaagtggcca gcttctgtcg agctctccat ccgggtgcgg tccgaagagg
     1681 tcggcctcgg ggtatttcgt gtccggggcg ggaccgaacg cgtgcccgtg ccccaccaga
     1741 cctgttgact tcacgctggc gcgagcctca ttgagactgt ccgggttgct cgcgacgggc
     1801 catgaaccgg ggcctgcagg accgtgaccg gagtgggagc cggggggttg ggggggcgga
     1861 gcgcagcgca gcccccgatc aacagatcga gggtggtcga gtggctggcg agcatcaccg
     1921 acaccctgat ggccgaatgg acacccgacc acgtggcgtt cttcgtcgac catcgcctgg
     1981 tcaagagggt gagcagtccc ccgcctatcc gatgcaggtg atgctcggcg tctatgggct
     2041 ccccgacgac gacgggcggg cagggcgtcc gggacggccg tatccgaagc agttcgtggt
     2101 cgactacttc cgcggctacc gactcaccag cccgcaaccg gtcaggtcct ggtccgaagg
     2161 ccgccgctca cgccgctcac atggtgttga catctcgggc cggcatactc gggtgcatgc
     2221 gactgctggt ggtggaggac gagacccggc tggcgctgct gctcaagcgt gggctcgagg
     2281 agcagggcta tgccgtggac gtgaccggcg acggtgccga ggcgctgtgg tacgccaccg
     2341 aggtcgacta tgacgcgatc gtgctggaca tcatgctgcc gagcatggac gggctggagg
     2401 tgaccaggcg gctgcgccgc ggtgggcgct gggtgcccgt gctgctgctg accgcccgtg
     2461 acgccgtcga tgcccgggtg gtcggtctgg atgccggcgc cgacgattac ctggtcaagc
     2521 cgttcagctt cgcggagctg gcggccaggg tgcgggcgct ggtccgccgc ggccgggtcg
     2581 agcggcccgc ggtgctggag gtcggggacc tccgcttgga cccggcgcgg cggcaggcct
     2641 ggcgcgggca ggtcgagctg gacctgtcgc ccacggagtt cgcgctgttg gagctgttcc
     2701 tgagccaccc cgacgaggcg ctgacccgca ccaggatcct ggagcacgtc tgggacttcg
     2761 cctacgaccc ggcctccaac gtggtcgacc agtacgtggc ctacctgcgc cgcaagatcg
     2821 accggccctt cggccgggcc gacctggaga cc
//
LOCUS       DASQQQ010000343         6857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_325442, whole genome shotgun sequence.
ACCESSION   DASQQQ010000343 DASQQQ010000000
VERSION     DASQQQ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325442"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="VE646_12765"
     CDS             complement(<1..1285)
                     /locus_tag="VE646_12765"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HZD80895.1"
                     /translation="MTALDDWGFGANSWLVDEMARRYEADPASVGPEWRALFDGGTGG
                     SSTVEERRPPAAPPAEEPAPEGWAPLPGPAQALARTMAQSLQVPTATSIRTVPAKLLQ
                     VNRRRLNRYLLRTLGGRVSYTHLIGWAVTRAAVSCSALRRVYAEVGGRPGTFTRPGVD
                     LGIAVDVLRSDGTRALMAPVVRGAGSLGFAGFVAAYDDLVARARSGRISPDELVGPGI
                     TLTNPGTIGTSASVPRLPEGQSAIVGVGAVGAPPEYAAADPDRLAEVGVGPVTTLTCT
                     YDHRVIQGAESGEFLRLVGALLLGKDDFYGEVFAGLEVPFRPIRWGRDVVRVADPVGE
                     AERHARVLQLINNYRVRGHLIAHLDPLATSPPETHPELDPATLGFSLWDLDRRFATGG
                     LADRHEATLEEVWDILRDAYCGTLSVEYMHIQEPDQ"
     gene            complement(1379..1981)
                     /locus_tag="VE646_12770"
     CDS             complement(1379..1981)
                     /locus_tag="VE646_12770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZD80896.1"
                     /translation="MNLQRRFYERSYRRGTPPWDTRITPPEVVQIVEGRDALGQGRAL
                     DLGCGTGTNLLYLAEHGWRAVGVDFSQIAIRTARKRTAAVPGIEVVRGDVTRLAELGV
                     GGAFDLVLDIGCFHSVPRRRRAAYADGVRRVTPPGATYLVFAFARWRGLRRLGIEDRR
                     SEMERRFGNGFDLVEVIPGREPAGAAWYRFVRRSAERGRR"
     gene            complement(2004..2987)
                     /locus_tag="VE646_12775"
     CDS             complement(2004..2987)
                     /locus_tag="VE646_12775"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZD80897.1"
                     /translation="MKGLVLEGPGEVAVRDVPEPEPAEGEVLLEVVACGVCGSDLTLI
                     KGGFPPGGVIGHEAVGRVLSAPAETGFREGQLMAVCPLDFCGVCEHCRAGRGQLCADA
                     VPNGLGFWRQGGLAERIAVPASMCAPMTGVDPLDATFVDPLAVTIHAVARSGRTAGPA
                     AVIGLGSVGISMLTACLSEGLTPVAGVDPIPEKRDLALSVGAVAAAAPGDQAGLSASL
                     GGPPEVVFECSGRPETIPEAANLVVKGGAVVLVGIYDLLSMTPLPLIVNEIDLRPSFC
                     YDRSEWERSVQLISTGAVKLSPMAETAPLEKLPTLIEEMKQGRAKKIIAIP"
     gene            complement(3082..4185)
                     /locus_tag="VE646_12780"
     CDS             complement(3082..4185)
                     /locus_tag="VE646_12780"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZD80898.1"
                     /translation="MNRLERMVRAIDRFQRRHVVLAFPFGVVKKFGDDQAGTLAALIA
                     YYGFFSLFPLLLVFVSVLGIVLSGHPGLREAILRSALGTFPVIGNEIGNKAGIHALTG
                     SWLTVVLGTAGALWAGLGVAQAAEKAMNTVWDIPKAEWPNFIFRRVRALGMLALLGTI
                     VIVSTFTSGWGSSGALSSLLLSIGGWAVSFLLNLVLFLITYQVLTARQLRWLDVLPGA
                     VIAAVAWTVLQAVGGFYVTHEVSSSSNVYGTFALVIGILIWISLGAQLTLFCAEINVV
                     RKRHLWPRSLVQPPINSGDKEVYRAIVHRARMRPEVAVRVWFTQDHEPGRNEGPIRGG
                     GPNLEHVPRTEERTEALADREGPEDPVPPPRSG"
     gene            complement(4391..4825)
                     /locus_tag="VE646_12785"
     CDS             complement(4391..4825)
                     /locus_tag="VE646_12785"
                     /inference="COORDINATES: protein motif:HMM:NF015720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HZD80899.1"
                     /translation="MYRRTTNRTNSVAAGFDWVNLLAAIWLFIAPWVLTFTGNAGAAW
                     DAWLTAIGIFVVALTALSTRRSMVPTGTEGINVLLGIWLFVSPWVLTFTGLTAAAWNA
                     WATGVLVTAASLGAMFTSGGRRRETLPAQPHEAEDVRRRHAA"
     gene            4979..5293
                     /locus_tag="VE646_12790"
     CDS             4979..5293
                     /locus_tag="VE646_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80900.1"
                     /translation="MAGNTKDAWDEVGERFAEWGRQLSARYREKERERAAPSVAEDRR
                     KLEEAAQAVVRQLDLAFTSLGETLRDPQAKQSLSEAGRAFREALSTTVSEAGERIRKR
                     TG"
     gene            5298..6710
                     /locus_tag="VE646_12795"
     CDS             5298..6710
                     /locus_tag="VE646_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HZD80901.1"
                     /translation="MAPRRAIIATVPRFHTIIEPFRIHSVEPLRFLTERERAERVREA
                     GYNLFNLRAEDVLIDLLTDSGTGAMSRDQWAAIQHGDESYAGSPSWFRFEEAVRTLFP
                     FRHIIPTHQGRAAEKILFTAIAGAGKVVPNNTHFDTTRANVEFTGAEALDLVIPEGRD
                     PAALHPFKGNVDLEALEALLEERAEAVPVVFVTLTNNSGGGQPVSMENLRGVRTLCDR
                     SGVPLFLDACRFAENAWFVKLREPGYAERSVPDIVREMAALAHGMTMSAKKDPMANIG
                     GWLAMDDDAWAERCRNLLILTEGFPTYGGLAGRDLEAIAQGLRECVDEPYLRYRIRST
                     EYLGEALDRAGIPIVKPVGGHAVYLDARALLTHVPPLEYPGQSLAVALYLAGGIRGCE
                     IGTVMFGRHPDGTESPAAMDLVRLAIPRRTYTQSHIDYVIEVVRAVAADARSLPGFRI
                     VEEPRQLRHFTARFEPMDRG"
     gene            6722..>6857
                     /locus_tag="VE646_12800"
     CDS             6722..>6857
                     /locus_tag="VE646_12800"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HZD80902.1"
                     /translation="MGRTAVVAIGGNSLIKDKMHQSVRDQYIAAGETCWHIAGMIRQG
                     W"
BASE COUNT         1074 a         2515 c         2327 g          941 t
ORIGIN      
        1 tctgatccgg ctcctggatg tgcatgtact ccacgctcag cgtgccgcag taggcatcgc
       61 ggaggatgtc ccacacctcc tccagcgtcg cctcgtgccg atcggccagt ccgccggtgg
      121 cgaaccgacg atccaggtcc cacaggctga acccgagcgt ggccggatcg agctcgggat
      181 gggtctcggg cggcgaggtg gccaacggat cgaggtgggc gatcaggtga ccgcgcaccc
      241 ggtagttgtt gatcagctgc aggacgcggg cgtggcgctc ggcctcgccc accgggtcgg
      301 ccacccggac cacgtctcgc ccccaccgga tgggccggaa gggcacctcc agcccggcga
      361 agacctcccc gtagaaatcg tccttcccca gcaggagcgc acccaccagt cgcaggaact
      421 ccccgctctc cgctccctgg atcacccggt ggtcgtaggt gcaggtgagc gtggtcaccg
      481 gccccacccc tacctccgcc aagcggtcgg ggtccgccgc cgcgtactcg ggaggggcgc
      541 ccaccgcccc cacgccgacg atggccgact gcccctccgg caggcggggg accgaggcgc
      601 tggtgccgat cgtgcccggg ttggtgaggg tgatgccggg accgaccaac tcgtcgggcg
      661 agatccgtcc ggaccgagcc ctggccacca gatcgtcgta cgcggccacg aagccggcga
      721 accccaggga ccccgccccc cggaccacgg gggccatcag ggccctggtc ccgtccgagc
      781 gaaggacgtc cacggcgatg cccagatcca cgcctgggcg ggtgaaggtg cccggccgtc
      841 cgcccacctc ggcgtacacg cggcggaggg ccgagcacga cacggcggcc cgggtcaccg
      901 cccacccgat gaggtgcgtg tatgagaccc ggcccccgag cgtgcgcagc aggtagcggt
      961 tcaggcgtcg ccggttgacc tggagcagtt tggccgggac cgttcggatc gaggtcgcgg
     1021 tcgggacctg gaggctctgc gccatggtcc gagccagggc ctgcgccggc ccgggcagcg
     1081 gagcccatcc ttccggcgcg ggctcctcgg ccgggggagc ggcgggcggc cgacgttcct
     1141 ccacggtcga tgatccaccg gtcccgccgt cgaacagggc acgccactcc gggccgaccg
     1201 aggcggggtc ggcctcgtac cggcgagcca tctcatcgac cagccaggag ttcgctccga
     1261 acccccagtc atcgagcgcg gtcatcgcct cccagcgtcc cccttgggtt ccgatgtcct
     1321 tcctataccc gcgggcggga tcacggccac gccgccccgc gccactcgga cctccgggtc
     1381 accggcggcc acgctcggcc gatcggcgga cgaaccggta ccaggcggcc ccggccggct
     1441 cccgtcccgg gatcacttcg accaggtcga acccgttccc gaaccgacgc tccatctcgc
     1501 tccgacggtc ctcgatcccc aggcgccgaa ggccccgcca cctggcgaag gcgaacacca
     1561 ggtacgtcgc cccggggggc gtcaccctcc gcaccccgtc cgcgtacgcc gcccgccgac
     1621 ggcgggggac gctgtggaag cacccgatgt ccagcacgag gtcgaaggcc ccacccacgc
     1681 ccagctcggc caggcgcgtg acgtcgcctc ggaccacctc gatccccggg accgccgccg
     1741 tccgcttcct ggcggtccgg atcgcgatct gggagaagtc cacccccacc gcccgccatc
     1801 cgtgctcggc gaggtagagc aggttggtcc cggtcccgca ccccagatcg agcgcccgtc
     1861 cctggcccag ggcgtcccgg ccctccacga tctggaccac ctcgggcggc gtgatccggg
     1921 tgtcccaggg cggggtgccc cggcggtacg agcgttcgta gaagcggcgc tgcaggttca
     1981 tgccggcggg ccgggttcct ggctcagggg atcgcgatga tcttcttggc tcgtccctgc
     2041 ttcatctcct cgatgagggt cggaagcttc tcgagcggag ccgtctcggc catcggggac
     2101 agcttcaccg cccccgtgga gatgagctgg acggaacgct cccactcgga ccggtcgtag
     2161 cagaaggagg gccgaaggtc gatctcgttc acgatgagcg ggagcggcgt catcgagagc
     2221 aggtcgtaga tccccaccaa cacgacggcc ccgcccttga ccacgaggtt ggccgcctcg
     2281 gggatcgtct cgggccgtcc gctgcactcg aagaccacct ccggcggccc acccaggctg
     2341 gcgctcagtc cggcctgatc gcccggggca gctgccgcca ccgcgcccac cgacagtgcg
     2401 aggtcgcgct tctccgggat cgggtccacg cccgccaccg gggtgaggcc ctccgagagg
     2461 caggcggtga gcatcgagat gcccacggat ccgaggccga tcaccgcggc cggaccggcg
     2521 gtccgtcccg agcgggccac cgcgtggatc gtgacggcca ggggatcgac gaaggtggcg
     2581 tcgagcggat ccaccccggt catcggggcg cacatcgagg cggggacggc gatccgctcc
     2641 gccaaccctc cttggcgcca gaagccaaga ccattgggca ccgcgtcggc acagagctga
     2701 ccccggcccg ctcggcagtg ctcgcagacc ccgcagaagt ccagggggca gaccgccatc
     2761 agttgaccct cccggaaccc ggtctcggcc ggagccgaca ggacgcggcc caccgcctcg
     2821 tggccgatca ccccaccggg agggaagccg cctttgatca gggtcaggtc cgatccgcac
     2881 acgccacagg caacgacctc gagcagcacc tcgccctcgg ccggctccgg ctccggcacg
     2941 tcgcggaccg ccacctctcc cggcccctcc agcacaagcc ccttcatgcc cactcagcct
     3001 atcgcgggga ccccggcggg gtctcgaggt cggccgaggg gatgtggggg ggatcccggc
     3061 cgcacggaag gatcgggcgc tctagcccga tcgcggcgga ggcaccgggt cctccggccc
     3121 ctctcgatcc gccagcgcct cggttcgctc ttccgttcgg gggacgtgct ccaggttcgg
     3181 cccgccccct cggatcggac cctcgttgcg gccgggctcg tggtcctgcg tgaaccagac
     3241 ccggaccgcc acctcgggtc gcatcctggc ccgatgcacg atggctcggt agacctcctt
     3301 gtccccgctg ttgatgggag gctggacgag gctcctcggc cacaggtgcc gcttgcggac
     3361 cacgttgatc tccgcacaga acagcgtcag ctgcgccccc aggctgatcc agatcaggat
     3421 cccgatcacc agggcgaacg tgccgtaaac gttgctggag ctgctgacct cgtgcgtcac
     3481 gtagaagccg ccgacggcct gcaggaccgt ccacgccacg gccgcgatca ccgcaccggg
     3541 cagcacgtcg agccaccgga gctgccgcgc ggtgagcacc tggtaggtga tgaggaacag
     3601 caccaggttc aggagaaacg agacggccca tcccccgatc gacaacagca gcgaggacag
     3661 cgcgcccgag cttccccagc cgctcgtgaa cgtggagacg atcacgatcg tccccaggag
     3721 cgccagcatc ccgagcgccc ggacccggcg gaagatgaag ttgggccact cggccttggg
     3781 gatgtcccag accgtgttca tggccttctc ggcggcctga gccaccccca gccccgccca
     3841 cagcgcccca gcggtgccga ggaccaccgt gagccagctg ccggtcagcg catggatgcc
     3901 ggccttgttc ccgatctcgt tcccgatcac ggggaacgtg cccaacgcgg acctcaggat
     3961 cgcctcccga agcccaggat ggccgctcag gacgatcccc agcacgctga cgaagaccag
     4021 gagcaggggg aacagcgaga agaacccgta gtaggcgatg agcgcggcca gggtcccggc
     4081 ttgatcgtcg ccgaacttct tgaccacgcc gaacgggaac gccagcacca cgtgccgacg
     4141 ctgaaagcga tcgatcgctc ggaccatccg ttccaggcgg ttcacagccg atgtgttccc
     4201 gcgccccgcg ccggacacac gccctgtggc aaggtccggg cccggttccg catgcgagga
     4261 gccgccccgg ctgctcgccg gagcggctcc ctggatcgct catcgggcgg cgacccgctt
     4321 ccgccctcac ctcatcgatc ggtcgaccga tgatcgaagg ccagcctcag gcccgcctcc
     4381 cccgccggga tcaggcggcg tggcggcgac gaacgtcttc cgcctcgtgc ggctgggccg
     4441 ggagcgtctc ccggcgtcgc ccgcccgacg tgaacatggc gccgagcgag gctgccgtca
     4501 ccagcacacc ggtggcccag gcgttccacg cggccgcggt cagaccggtg aaggtcagga
     4561 cccacggcga cacgaacagc cagatcccca gcaggacgtt gatcccttcg gtcccggtgg
     4621 gcaccatcga acggcgcgtc gacagcgcgg tcagcgccac gacgaagatg ccgatggcgg
     4681 taagccaggc gtcccaggcg gccccagcgt tcccggtgaa cgtgaggacc cagggcgcga
     4741 tgaacagcca gatcgcagcg aggaggttga cccagtcgaa ccccgccgca accgagttgg
     4801 tgcggttcgt agttcgtctg tacatcgcat cctccctctt gttgtcttcc ttcgcggctc
     4861 tcacgtggcg tccacatagg gaccgccatc gcgaaaccgc cttcgcccat cagctttccc
     4921 gttctgtacg ttccgtacac aactggcggt gagcgctcac gggcgtagac tcgaggccat
     4981 ggcagggaac acgaaggacg cgtgggacga ggtcggggaa cggttcgcgg aatggggccg
     5041 tcagctctcg gcccgctacc gcgagaagga gcgcgagcgg gcagccccga gcgtcgccga
     5101 ggatcgccgc aagctcgagg aggccgcaca agccgtggtc cgccagctcg acctggcgtt
     5161 cacctccctc ggtgagacgc tccgcgatcc tcaggccaag cagagcctga gcgaagccgg
     5221 gcgcgccttc cgcgaggcgc tgtccacgac cgtctccgag gcgggggagc ggatccgcaa
     5281 gcgcaccggc tgatcaggtg gcgccgcggc gtgccatcat cgccaccgtg ccgcggttcc
     5341 acacgatcat cgagccgttc cggatccact cggtggagcc gctgcgcttc ctgaccgagc
     5401 gcgagcgggc cgagcgggtg cgcgaggccg ggtacaacct gttcaacctg cgcgcggagg
     5461 acgtgctgat cgacctgctg accgactcgg gcaccggggc gatgagccgg gaccagtggg
     5521 cggcgatcca gcacggtgac gagtcctacg caggctcccc ctcctggttc aggttcgagg
     5581 aggccgtccg cacgctgttc ccgttccggc acatcatccc gacgcatcag ggccgcgcgg
     5641 ccgagaagat cctcttcacg gcgatcgccg gagccggcaa ggtggttccg aacaacaccc
     5701 acttcgacac cacccgagcc aacgtggagt tcacgggggc cgaagccctg gatctggtga
     5761 tccctgaggg ccgggacccc gccgccctcc atcccttcaa gggcaacgtg gacctggagg
     5821 cgctcgaggc cctgctggag gagcgggccg aggcggtccc ggtggtgttc gtgaccctca
     5881 cgaacaactc gggtggtggg caaccggtgt ccatggagaa cctgcggggc gtccggacgc
     5941 tgtgcgaccg gtccggggtg ccgctgttcc tggacgcctg ccgcttcgcc gagaacgcct
     6001 ggttcgtcaa gctccgggag cccggctacg cggagcgcag cgtgcccgac atcgtccggg
     6061 agatggccgc gctcgcccac gggatgacga tgagcgcgaa gaaggacccc atggcgaaca
     6121 tcggcggctg gttggccatg gacgacgacg cctgggccga gcgctgccgc aacctcctga
     6181 tcctcaccga ggggttcccc acctacggcg gcctggccgg gcgcgacctg gaggccatcg
     6241 cccagggcct gcgcgagtgc gtggacgagc cctacctgcg ctaccggatc cgctccaccg
     6301 agtacctggg cgaggccctg gaccgggctg ggatccccat cgtcaagccg gtggggggcc
     6361 acgccgtcta cctcgatgcc cgggccctcc tgacgcacgt ccctcccctg gagtacccgg
     6421 gccagtccct ggccgtggcg ctgtacctgg cgggcgggat ccggggctgt gagatcggca
     6481 cggtgatgtt cggccggcac ccggacggca cggagtctcc cgccgcgatg gacctggtcc
     6541 gcctggcgat cccgaggcgg acctacacgc aatcccacat cgactacgtg atcgaggtgg
     6601 tgcgtgcggt ggcagccgat gcgcggtccc tgcccggctt ccggatcgtg gaggagcccc
     6661 gccagctgcg gcacttcacc gcgaggttcg agcccatgga ccgaggatga ggagaaccgc
     6721 catgggcagg acggcagtgg tggctatcgg cgggaactca ctgatcaagg acaagatgca
     6781 ccagagcgtc cgggatcaat acatcgcggc cggtgagacc tgctggcaca tcgcggggat
     6841 gatccgccag gggtggg
//
LOCUS       DASQQQ010000344         6809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_302743, whole genome shotgun sequence.
ACCESSION   DASQQQ010000344 DASQQQ010000000
VERSION     DASQQQ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6809
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302743"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3254
                     /locus_tag="VE646_12805"
     CDS             <1..3254
                     /locus_tag="VE646_12805"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZD80903.1"
                     /translation="EVLTAFRGLSVKQLVADDAVYRSLRERFGDYFEGGMGAEAIKRL
                     LMDLKLEEEAENLREQIATAKGTRRLKAIKRLKVVSNFTEGTNSPMGMVLDAVPVIPP
                     DLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLQAPEIIVNNEKRMLQEAVDA
                     LFDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPQLKL
                     HQCGLPKQMALELFKPFVMKRLVDLNLAQNIKSAKRMVERARPQVWDVLEEVIREHPV
                     LLNRAPTLHRLGIQAFEPVLVEGKAIQIHPLVCTAFNADFDGDQMAVHVPLSSEAQAE
                     ARVLMLSANNILSPAHGRPIAVPTQDMVLGIYYLTIEPERPKKDEDIPRFSSVSEAMM
                     ALDHRQVKLHTVIKVRLPGKNLPPEMLPDTVDATEDGRVPLVETTIGRLLFNQCFPDE
                     MEYVNWALVKGDIGKLVDQCVHRFDRATVEQILDALKAVGFHYATRAGVTLGVEDVTT
                     PSDKAKILDNYENRAQKIENQYRKGIITDDERRQELIEIWTQATDDVKDAMEAQFGVT
                     NPIYMMANSGARGNIMQIRQIAGMRGLVANPKGEIIPRPIKSNFREGLSVLEYFISTH
                     GARKGLADTALRTADSGYLTRRLVDVSQELIVREEDCGTDRGFTIPVTYETPTGARVP
                     NAHLDTALFGRILAEDVKVERRKVASAGELLTDTLMDQLVEEGVDTIRVRTPMSCEAD
                     YGVCRMCYGTSLATGRMVDIGESVGIIAAQSIGEPGTQLTMRTFHTGGVAGEDITHGL
                     PRVVELFEARRPKGEALITELPGTVGVEDDDEKKERRITVTSEDEDQVQYKVSFRARL
                     LVGDGDEVDVGQQLTEGSVNPHEKLRIEGVSALQAHLVDEVQQVYRSQGVTIHDKHIE
                     LIVRQMLRKVQIIEPGDTDFLPGDLVDRRRFESLNRATVENGGMPATARPQLLGITKA
                     SLATDSWLSAASFQETTRVLTEAAIAAKSDSLLGLKENVIIGKLIPAGTGMSRYRNVQ
                     VRIKPEAIPEYWLVRQRELAEQAAAAEAEPALTGLTREEAEQMLGGSLSPAEE"
     gene            complement(3281..3469)
                     /locus_tag="VE646_12810"
     CDS             complement(3281..3469)
                     /locus_tag="VE646_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80904.1"
                     /translation="MEGIRCARLTREGRARINDCRTALGGTRRKIADTDLEARARVVR
                     DLGTIERALSRPLDRWWL"
     gene            complement(3476..4300)
                     /locus_tag="VE646_12815"
     CDS             complement(3476..4300)
                     /locus_tag="VE646_12815"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HZD80905.1"
                     /translation="MTPPSDPGSLDHLLAAAERAALRGGEVVRAGVGTSEATTKGAGD
                     YVTEVDRSSEQAIGQVLREAAPGIAMLGEEEGWAAGSGAGRYWVVDPLDGTTNFLHRF
                     PAVGVSVALVEDGRPVVGVVHSPFLGETALAVAGGGARRRREGRADEALHVSTRPPER
                     GVIGTGFPFRRKELLERYLRMFEGALRRFEDLRRPGAAALDLAWVAAGVFDGFFELSL
                     SPWDMAAGAVLIREAGGVVSDWDGEPDGYLSTGHILAGSPAVHEVLLELASQGGGG"
     gene            4610..4984
                     /gene="rpsL"
                     /locus_tag="VE646_12820"
     CDS             4610..4984
                     /gene="rpsL"
                     /locus_tag="VE646_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HZD80906.1"
                     /translation="MPTIQQLVRNGRQRPKGKTKSPALRGSPQRRGVCTRVYTATPKK
                     PNSALRKVARVRLTTGVEVTAYIPGVGHNLQEHSIVLVRGGRVKDMPGVRYKIVRGTL
                     DTAGVRDRKKARSRYGAKKGGE"
     gene            4987..5457
                     /gene="rpsG"
                     /locus_tag="VE646_12825"
     CDS             4987..5457
                     /gene="rpsG"
                     /locus_tag="VE646_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HZD80907.1"
                     /translation="MPRKGPAVRREIEPDPVYQNQLVTQLINKVLLDGKKSLAERTVY
                     KALEVISERTANDPVITLKRAVENVRPMVEVRSRRVGGANYQVPVEVRPTRGTTLALR
                     WMVNNARARKEHSMSDRLVGEIMDASQGQGVSVKRREDMHKMAEANRAFAHYRW"
     gene            5651..>6809
                     /locus_tag="VE646_12830"
     CDS             5651..>6809
                     /locus_tag="VE646_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018217064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZD80908.1"
                     /translation="MAHIDAGKTTTTERILYYTGKAYKIGEVHEGTAQMDWMVQERER
                     GITITSAATTTHWKGHWINIIDTPGHVDFTVEVERSLRVLDGAIAVFDAVAGVEPQTE
                     TVWRQADRYRVPRICFVNKMDRTGADFGRTVQMMVDRLVANPLVVQLPWGVEGDFTGV
                     IDLVEMRAHRWTSEMGEEWETVEIPEELREAAVEAHRELFEKLADHDESLMEKFVHEQ
                     EPTQEELRRAIRRATLSGEGVPVLCGSAFKNKAIQPLLDAIVNYLPAPIDVPPVQGHT
                     PAGEPAERAPDDNEPFSGLVFKIMSDPYVGRLTYVRVYSGVLRQGSHVQNSTKDRKER
                     IGRILQMHANHREDKDAAFTGDIVAVVGLKHSTTGDTLCEPAEPIVLESISF"
BASE COUNT         1162 a         2377 c         2391 g          879 t
ORIGIN      
        1 acgaggtcct gaccgcgttc aggggcctgt ccgtgaagca gctggtggcc gacgacgccg
       61 tgtaccggtc cctgcgggag cgcttcggcg actacttcga gggcggcatg ggcgccgagg
      121 cgatcaagcg cctgctgatg gacctcaagc tggaggagga ggccgagaac ctgcgcgagc
      181 agatcgccac ggccaagggc acccgccggc tgaaggcgat caagcgcctg aaggtggtct
      241 cgaacttcac cgagggcacg aactcgccca tgggcatggt gctcgacgcc gtgccggtga
      301 tcccgccgga cctgcgcccc atggtgcagc tggacggcgg ccggttcgcc acctcggacc
      361 tgaacgacct gtaccgccgg gtcatcaacc gcaacaaccg gctgaagcgg ctgctggacc
      421 tgcaggcccc cgagatcatc gtgaacaacg agaagcggat gctgcaggag gccgtggacg
      481 cgctgttcga caacggccgc cggggccgtc cggtcaccgg gccgggcaac cggcccctga
      541 agtcgctctc ggacatgctg aagggcaagc agggccggtt ccgccagaac ctgctgggta
      601 agcgggtgga ctactcgggc cgctcggtga tcgtggtcgg cccgcagctc aagctgcacc
      661 agtgcggcct gcccaagcag atggccctgg agctgttcaa gccgttcgtg atgaagcggc
      721 tggtggacct caacctggcc cagaacatca agagcgccaa gcggatggtg gagcgggccc
      781 gcccgcaggt gtgggacgtg ctcgaggagg tcatccgcga gcacccggtc ctgctgaacc
      841 gggcgcccac gctgcaccgg ctggggatcc aggccttcga gccggtcctg gtggagggca
      901 aggccatcca gatccacccg ctggtctgca cggcgttcaa cgcggacttc gacggcgacc
      961 agatggccgt ccacgtcccg ctgtcctccg aggcgcaggc cgaggcccgg gtgctgatgc
     1021 tctcggccaa caacatcctg tccccggcgc acggccgtcc catcgccgtg cccacccagg
     1081 acatggtgct ggggatctac tacctgacga tcgagccgga gcgtcccaag aaggacgagg
     1141 acatcccccg gttctcctcg gtgagcgagg ccatgatggc cctggaccac cggcaggtga
     1201 agctccacac ggtgatcaag gtgcggctgc cgggcaagaa cctgccgccc gagatgctgc
     1261 cggacacggt ggacgccacc gaggacggcc gggtgcccct ggtggagacc acgatcggcc
     1321 ggctgctgtt caaccagtgc ttccccgacg agatggagta cgtgaactgg gccctggtga
     1381 agggcgacat cggcaagctg gtggaccagt gcgtgcaccg gttcgaccgg gccaccgtgg
     1441 agcagatcct ggacgccctg aaggcggtgg gcttccacta cgccacccgc gccggcgtga
     1501 cgctcggcgt ggaggacgtc accacgccct cggacaaggc caagatcctg gacaactacg
     1561 agaaccgcgc ccagaagatc gagaaccagt accgcaaggg catcatcacg gacgacgagc
     1621 ggcgccagga gctcatcgag atctggaccc aggccaccga cgacgtgaag gacgccatgg
     1681 aggcgcagtt cggcgtgacg aaccccatct acatgatggc caactcggga gcccgaggga
     1741 acatcatgca gatccgtcag atcgccggga tgcgtggcct ggtggccaac ccgaagggcg
     1801 agatcatccc tcggcccatc aagtcgaact tccgggaggg gttgtccgtg ctggagtact
     1861 tcatctccac gcacggcgcc cgcaagggcc tggccgacac cgccctgcgg acggccgact
     1921 cggggtacct cacgcggcgg ctggtggacg tgtcccagga gctcatcgtg cgcgaggagg
     1981 actgcggcac ggaccgcggg ttcacgatcc cggtgacgta cgagacgccc accggggccc
     2041 gggtgccgaa cgcgcacctg gacaccgcgc tgttcgggcg gatcctggcc gaggacgtga
     2101 aggtggagcg gcgcaaggtg gcctcggccg gcgagctgct gaccgacacg ctgatggacc
     2161 agctggtgga ggagggcgtg gacacgatcc gcgtccgcac cccgatgtcc tgcgaggccg
     2221 actacggggt ctgtcggatg tgctacggca ccagcctggc caccgggcgg atggtcgaca
     2281 tcggcgagtc ggtggggatc atcgcggcgc agtcgatcgg tgagccgggg acccagctga
     2341 ccatgcggac gttccacacc ggcggcgtgg ccggcgagga catcacccac ggtctgcctc
     2401 gggtggtcga gctgttcgag gcccggcgcc ccaagggcga ggccctgatc accgagctgc
     2461 cgggcaccgt cggcgtcgag gacgacgatg agaagaagga gcggcggatc acggtcacgt
     2521 ccgaggacga ggaccaggtc cagtacaagg tgtcgttccg ggctcggctc ctggtgggcg
     2581 acggcgacga ggtcgacgtg gggcagcagc tcaccgaggg ctcggtcaac ccgcacgaga
     2641 agctgcggat cgagggggtc tcggccctgc aggctcacct ggtggacgag gtccagcagg
     2701 tgtaccggtc ccagggcgtg acgatccacg acaagcacat cgagctgatc gtgcggcaga
     2761 tgctgcgcaa ggtgcagatc atcgagcccg gcgacacgga cttcctcccg ggcgacctgg
     2821 tggaccgccg ccggttcgag agcctgaacc gggcgaccgt ggagaacggc gggatgccgg
     2881 ccacggcccg gccgcagctg ctggggatca ccaaggcgtc gctggccacg gactcgtggc
     2941 tgtcggcggc ctcgttccag gagaccacgc gggtgttgac cgaggccgcg atcgccgcga
     3001 agtcggactc cctgctgggt ctgaaggaga acgtgatcat cggcaagctg atcccggccg
     3061 ggacggggat gtcgcggtac cgcaacgtgc aggtgcggat caagcccgag gcgatccccg
     3121 agtactggct ggtccgccag cgggagctgg ccgagcaggc cgcggccgcg gaggccgagc
     3181 cggctcttac cggcctgacc cgcgaggagg ccgagcagat gctgggcgga tccctcagcc
     3241 cggccgagga gtaggggtca gagcggccgg gcggtagggg tcagagccac cagcgatcga
     3301 gcgggcgcga cagcgcccgc tcgatcgttc cgaggtcccg caccacgcgc gcgcgggcct
     3361 cgaggtcggt atcggcgatc ttccgcctgg tcccgccgag cgcggtccgg cagtcgttga
     3421 tccgggcgcg cccctctcgg gtgagccggg cgcaccggat gccctccatg cgccctcacc
     3481 cacccccacc ctgcgacgcc agctccagca ggacctcgtg cacggccggg gatcccgcca
     3541 ggatgtgccc ggtggacagg tacccgtcgg gctcgccgtc ccagtcgctc accacacccc
     3601 ccgcttcgcg gatcagcacc gctcccgccg ccatgtccca cggcgacagc gacagctcga
     3661 agaacccgtc gaagaccccc gccgccaccc aggccaggtc cagcgccgcc gctcccggcc
     3721 gtcgtaggtc ctcgaagcgc ctgagcgccc cctcgaacat ccgcaggtag cgctcgagca
     3781 gctccttgcg ccggaacggg aacccggtcc cgatcacgcc ccgttcgggc ggccgggtgg
     3841 acacgtgcag cgcctcgtcg gcccgtccct cccggcgccg ccgggcgccg ccgcccgcca
     3901 cggccagcgc ggtctccccc aggaagggcg agtggaccac gcccaccacc ggccggccgt
     3961 cctccaccag cgccacggag acccccacgg cggggaaccg gtgcaggaag ttggtggttc
     4021 cgtccagcgg gtcgaccacc cagtaccgcc cggcaccgct cccggccgcc cagccctcct
     4081 cctcgcccag catcgcgatc cccggtgcgg cctcgcgcaa cacctggccg atcgcctgct
     4141 cggaggaccg gtccacctcg gtcacgtagt cgccggcgcc cttggtggtg gcctcggagg
     4201 tgccgacccc cgcccggacc acctcgcctc cgcgcagcgc ggcccgctcg gccgccgcca
     4261 gcaggtggtc cagggagccg ggatccgagg ggggcgtcat cgagcgctca cgctagcaga
     4321 cggcctgcta tcatcccgcc ccggcgcaac cgcggccccc cggcggcccg ggccggcgag
     4381 ggcgagggca gcggcaccac ccgttcgttg acggccgtgc ctcgtgccta gtagccttag
     4441 cggttgcgcc ccggacccgg caggcgggtc cggcatctag cgagcgttca gggggtaccc
     4501 ccgaagggac aactgtgccc gcggcagtcc ctgggagccg ggccttcggg tccggcttct
     4561 cggtgggagc cccaagaggg aacatggcga gagtgctgac gagagaacta tgcccacgat
     4621 ccaacagttg gtcaggaacg ggcgtcaacg ccccaagggc aagacgaaga gccctgcgct
     4681 ccgcggctcg ccccagcgcc ggggcgtctg cacgcgcgtc tacacggcca ccccgaagaa
     4741 gccgaactcg gctctgcgga aggtggcccg ggtccggctg accaccgggg tcgaggtcac
     4801 cgcctacatc cccggcgtgg ggcacaacct ccaggagcac tccatcgtcc tggtccgtgg
     4861 gggccgggtg aaggacatgc ccggtgtccg ctacaagatc gtccgaggca ccctcgatac
     4921 cgcgggggtc cgggatcgca agaaggcgcg ctcccgctac ggcgcgaaga agggtgggga
     4981 gtagccatgc cccgcaaagg ccctgccgta cgccgggaga tcgagcccga tcccgtctac
     5041 cagaaccagc tggtgaccca gctgatcaac aaggtcctgc tcgacgggaa gaagtccctg
     5101 gccgagcgca ccgtctacaa ggccctcgag gtcatcagcg agcgcaccgc caacgacccg
     5161 gtgatcaccc tgaagcgggc ggtggagaac gtccggccca tggtggaggt gcgctcgcga
     5221 cgggtgggcg gcgccaacta ccaggtgccg gtggaggtgc gacccacgcg gggcaccacc
     5281 ctggcgcttc ggtggatggt gaacaacgcc cgggcccgca aggagcactc gatgtccgat
     5341 cgcctggtgg gcgagatcat ggacgcctcc cagggccagg gcgtctcggt caagcgtcgc
     5401 gaggacatgc acaagatggc ggaggcgaac cgcgcgttcg cgcactaccg atggtgatga
     5461 tcgaggagaa cacccgtgcc gggctccagg gcccggcgtt ccacgagggg gtgagctagt
     5521 ggccaccccc gcggaggaga tcaacgtggc tgaggcggcc gagcagccga ggggacgtca
     5581 gaccggaggg tccaacaagg acctggtgct ccgggagtac cccctgggcc ggacccggaa
     5641 catcgggatc atggcccaca tcgacgccgg caagaccacc accaccgagc ggatcctcta
     5701 ctacaccggc aaggcctaca agatcggcga ggtccacgag ggcaccgccc agatggactg
     5761 gatggtccag gagcgcgagc gcggcatcac catcaccagc gccgccacca ccacgcactg
     5821 gaagggccac tggatcaaca tcatcgacac gcccggccac gtggacttca cggtcgaggt
     5881 ggagcgaagc ctgcgcgtgc tcgacggcgc gatcgccgtg ttcgacgccg tggccggcgt
     5941 ggagccccag accgagacgg tctggcgcca ggcggaccgc taccgggtcc cccggatctg
     6001 cttcgtgaac aagatggacc gcaccggcgc ggacttcggc cgcacggtcc agatgatggt
     6061 ggaccgcctg gtggccaacc ccctggtggt ccagctcccc tggggggtgg agggcgactt
     6121 caccggcgtg atcgacctgg tggagatgcg ggcccaccgc tggacctccg agatgggcga
     6181 ggagtgggag accgtggaga tccccgagga gctccgcgag gccgccgtcg aggcccaccg
     6241 cgagctgttc gagaagctgg ccgaccacga cgagtccctg atggagaagt tcgtccacga
     6301 gcaggagccc acccaggagg agctgcgccg ggccatccgc cgggccacgc tctcgggcga
     6361 gggcgtgccg gtcctgtgcg gcagcgcctt caagaacaag gcgatccagc ccctgctcga
     6421 cgccatcgtg aactacctgc cggcgcccat cgacgtgccc ccggtgcagg gccacacccc
     6481 ggccggcgag ccggcggagc gcgcgcccga cgacaacgag ccgttctcgg gcctggtgtt
     6541 caagatcatg tccgacccct acgtgggccg gctcacctac gtccgcgtgt actcgggcgt
     6601 cctgcgccag ggctcgcacg tgcagaactc caccaaggac cgcaaggagc ggatcggccg
     6661 catcctgcag atgcacgcca accaccgcga ggacaaggac gccgccttca ccggcgacat
     6721 cgtggccgtg gtggggctca agcactccac caccggcgac acgctgtgtg agccggccga
     6781 gcccatcgtg ctcgagtcga tcagcttcc
//
LOCUS       DASQQQ010000345         2894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_52422, whole genome shotgun sequence.
ACCESSION   DASQQQ010000345 DASQQQ010000000
VERSION     DASQQQ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52422"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VE646_12835"
     CDS             complement(<1..630)
                     /locus_tag="VE646_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80909.1"
                     /translation="MPVAEVGFTWQQALGAFAIITVAAFLVTWVLTDLLQIPRTPYIP
                     MLLVLALALGAGYVAWSGTSARELFSDGVGWGVAAGLIAAAMAVPLVQHLPPHPHPTG
                     SRLAELMVWEGLVYGIAEGVLLSTLPVLVIWQALGDLGWTDGGWAKIGAGVLAILGSL
                     LVILVHHLGYAEFRGKEGRMGLFGALGVCGIQAIAFLATGSVLAPMIAHI"
     gene            960..1205
                     /locus_tag="VE646_12840"
     CDS             960..1205
                     /locus_tag="VE646_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZD80910.1"
                     /translation="MLFDMYLSNPDVQGYALEEIAVPTLIISAKDDGLSAFENAARAA
                     TRIAGSKLVACERGGHLLLGDEGLVRAEIGSIVAASR"
     gene            1362..1691
                     /locus_tag="VE646_12845"
     CDS             1362..1691
                     /locus_tag="VE646_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80911.1"
                     /translation="MIEREERVARNEAISRDINEDLERAQGPLPGDPYARITCECGQA
                     GCERLIAITGSEYERVRSAPERFVVVPDHVDPAVEQVVEQTDRFVVVAKKEGAPREVA
                     EATDPRA"
     gene            complement(1688..2041)
                     /locus_tag="VE646_12850"
     CDS             complement(1688..2041)
                     /locus_tag="VE646_12850"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZD80912.1"
                     /translation="MPELRHALGELDLPPEMSEDARLLLTELVSNCIRHAGLRLAELI
                     LIRASWDGTRLRIDVRDGGAPGTQAPVAGAIRPDPGAESGWGLYLVDRLAARWGRASG
                     RYWFELERSGKDRGR"
     gene            2288..2716
                     /locus_tag="VE646_12855"
     CDS             2288..2716
                     /locus_tag="VE646_12855"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZD80913.1"
                     /translation="MRIQRSLPADPTAPRRARESLGALGHELRSRSDHDLRLVLSELV
                     TNAVKYGGLRKRERIEVDLWVGPTRSEVMVRYPQHAGFPPIAPPEPRGEEGWGLFLVD
                     HVADRWSVVECGGYLLIWVELTGEPGPERAPIVSASTYGG"
BASE COUNT          476 a         1014 c         1007 g          397 t
ORIGIN      
        1 gatgtgggcg atcatcggtg ccaggacgct gccggtcgcg aggaacgcga tggcctggat
       61 cccgcagacg cccagggcgc cgaacagccc catcctgccc tccttgcccc ggaactccgc
      121 gtagccgagg tggtgcacca ggatcacgag cagggagccc aggatcgcca ggaccccggc
      181 tccgatcttg gcccaccccc cgtcggtcca ccccagatca cccagggcct gccagatcac
      241 caggaccgga agcgtggaga gtaggacccc ctcggcgatc ccgtagacca acccttccca
      301 caccatcagc tcggccagcc tgctgccggt cgggtgcggg tggggcggga gatgctggac
      361 cagcgggacc gccatggcgg cggcgatcag cccggcggcg acgccccacc ccacgccgtc
      421 ggagaacagc tccctcgccg aggtgccgga ccaggccacg tagcctgccc cgagcgcgag
      481 ggcaaggacg agcagcatcg ggatgtacgg ggtgcgcggg atctgcaaca ggtcggtcag
      541 cacccaggtg accaggaacg cggccaccgt gatgatggcg aacgccccca gggcctgctg
      601 ccaggtgaag cccacttctg cgacgggcat gagaaccaca cctcccagtg ccgggaagga
      661 caaggcgttt gcccctctgt ccccatgctc atggaaccag ggtgcctcgg gcaggtccct
      721 ctcgccatgg gtccgggggc cgaacgtccc ggataggtcc caccaggcac gggggacgtc
      781 cggtgaggac gttcgccccg ttccgggtgg tcccgaagcc cccttccagc cggtggcgcc
      841 cggacggaga ctgccgccca tggggatgcc gtcgtccttc gagcccaccc ccgagcagcg
      901 gcgatccatc gaggaggtcc aagacagctt cttccgggtc catccccgca aagctggggg
      961 tgctcttcga catgtacctg tccaaccccg acgtgcaggg atatgccctg gaggagatcg
     1021 cggtaccgac gctgatcatc agcgccaagg atgatggttt gtcggccttc gagaacgccg
     1081 ctcgggcagc gacgcggatc gccggctcga agctggtcgc ctgtgagcga gggggccacc
     1141 tactgctggg cgacgagggt ctggtccggg ccgagatcgg atcgatcgtc gcagcatcaa
     1201 ggtagtcagc cccgcggcca gacccatacc ccccgcacct ctcctgctcg gggcacggcg
     1261 aggccgcttc tctcccccgt cctcctcccg tacagttctc cgagctactg gggtggggga
     1321 ggatccgggg tgccgacccc agcgatccga tggagggcgt gatgatcgag cgggaggagc
     1381 gggtggcccg caacgaggcg atctcgcgag acatcaacga ggacctggaa cgggcccagg
     1441 gacccctgcc aggtgatccc tacgcccgga tcacctgcga gtgcggccag gcggggtgcg
     1501 agcggctgat cgcgatcacc ggctcggagt acgagcgcgt gcgctcggcg ccggagcggt
     1561 tcgtcgtggt ccccgaccac gtcgatccgg cggtggagca ggtggtggag cagaccgatc
     1621 ggttcgtggt agtggccaag aaggagggtg ctccccggga agtggccgag gccaccgacc
     1681 cccgggccta gcgccctcga tctttgccgg atcgctccag ctcgaaccag tagcggccgc
     1741 tcgcccgtcc ccaccgggcg gccagccggt ccacgaggta caggccccat cccgactcgg
     1801 ccccgggatc gggtcggatc gcgcctgcca cgggagcctg ggtccccggc gctcctccat
     1861 cacgcacgtc gatgcggagc cgggtcccgt cccaggaggc ccggatcagg atcagctccg
     1921 caaggcgaag accggcgtga cggatgcagt tggagaccag ctcggtcaga agcagccgag
     1981 cgtcctccga catctcgggg ggaaggtcca gctcccccaa ggcgtggcga agctcgggca
     2041 aggctgacgg tacaggcgca acccccaact ccagcacccg cccgccgtca cgaggggcgg
     2101 tccgtccaga tcccatccga ctggatatgg cctacccacc gggtctggat ccattcgaga
     2161 gtgacaagag tccccgcccc cgtgagcgca ccccaccttc cgaggcgata tcacccaccg
     2221 gttcgtcccg ccggggacgg cgtttcgcgc ttcctgggga cgggtacgcg gatgctaggt
     2281 ggtgccgatg cgaatccaac gctcactgcc cgccgatccc accgcgcccc gacgggcgcg
     2341 ggagagcctg ggtgcgctcg gccacgagct gcgctcccgc agcgaccatg acctgcgctt
     2401 ggtgctgagc gagctggtca ccaacgcggt caagtacgga ggccttcgca agcgcgagcg
     2461 gatcgaggtg gatctgtggg tggggcccac ccggagcgag gtgatggtgc gctacccgca
     2521 gcacgccggg ttccccccga tcgcccctcc cgagccccgg ggagaggagg gatggggcct
     2581 gttcctggtg gaccacgtcg cggaccggtg gagcgtggtg gagtgcggcg gctacctgct
     2641 gatctgggtg gagctcaccg gcgagccggg ccctgagcgc gcacccatcg tctcggccag
     2701 cacctatggg ggatgatgcc agagcggcac tcggggactt cgaccaagtg gctctgcggg
     2761 cggtctcccg ccgccgagtg gccccacccg ggtcagaccg gctcagaccg gtggacccgg
     2821 atcaggccgc gaggagccga tcgaagaagg accggtagcg ctgcaacgcg acccgcagat
     2881 cctcggtgga gacc
//
LOCUS       DASQQQ010000346         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_271949, whole genome shotgun sequence.
ACCESSION   DASQQQ010000346 DASQQQ010000000
VERSION     DASQQQ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271949"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VE646_12860"
     CDS             <1..215
                     /locus_tag="VE646_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80914.1"
                     /translation="RAVLLGFHWLERPFPYRGPALASLHGRRGADLAARRFEETWGRA
                     HDIRPALQGILGRARDAARRLEEHPP"
     gene            328..1608
                     /locus_tag="VE646_12865"
     CDS             328..1608
                     /locus_tag="VE646_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80915.1"
                     /translation="MSGTPPRLGTRFVTVLVAASLGSCGDEAGPAGWEVTTDTVDGVV
                     RVENAPPESGPRPTLVAAEELRVGTVAGGGPASFGMIRSIAVLPDGRFAVADAQAEEV
                     RLFDGQGRHLRTFGGKGAGPGELQGMQGVYVDHEGLLRVAEQGNARLSVFHPDTGLVT
                     TYPLRLHSYGFRGPWPAAVDSAGRTLVASSGQYGEGRYWNMVRVYDPTMHQIDSIPYR
                     EYTDVARRATGGGSDDDFPGAWRIDLGNGGWTWAQVPFYAQPYEVLAPTGEFWSSAEG
                     RRELEIARWMPPGDTSLVLTSRRPPDPVTPAERDSAMTELRETLARRSPSPPSLDASR
                     VPATKPPLHGLSLDRAGRLWVRLTEPTADSTVYDVFGRDGRHAETVRLPFRVDRYVPP
                     LVRGDVLWAVVTDDMDVQYVVRAALRSPSRDTGP"
     gene            1839..1911
                     /locus_tag="VE646_12870"
     tRNA            1839..1911
                     /locus_tag="VE646_12870"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1872..1874,aa:Asn,seq:gtt)
     gene            complement(2097..>2365)
                     /locus_tag="VE646_12875"
     CDS             complement(2097..>2365)
                     /locus_tag="VE646_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HZD80916.1"
                     /translation="WTTESVEDRGDRLRIKVTSCANVDLFTAIGCPELGRLGCDHDLA
                     GYPVVLDRVDAEFRRPCTLAKGGDFCDFNFYRRGTAPTTAHLNR"
BASE COUNT          308 a          828 c          864 g          365 t
ORIGIN      
        1 cccgggccgt gctcttgggc ttccactggc tggagcgccc gttcccctac cggggcccgg
       61 ccctggcgag cctccacggc cgcaggggcg ccgacctggc ggcccgccgc ttcgaggaga
      121 cctggggccg cgcccacgac atccgcccgg cgctccaggg catcctcggt cgggcgcggg
      181 acgctgctcg gcggctggag gagcatccgc cgtgaggcgg gcggcgggtg gcgatgttct
      241 tgcacctgcg ctcctccgcc ggcattctgg cgacatccgc gcaggcggga agcctggcgg
      301 tgtcgtcgac ccagcacacc aagagcggtg agcggcactc ccccgagact cggcacccgg
      361 ttcgtgaccg tcctggtggc ggcctccctc ggttcctgtg gggacgaggc cggtccggcg
      421 ggctgggagg tcaccacgga cacggtggat ggcgtggtgc gggtggagaa cgcgccgccg
      481 gagtcgggac ctcggccgac gctggttgcc gcggaggagc tgcgtgtggg gacggtggcc
      541 ggaggtggac cggcctcctt cgggatgatc cgctccatcg ccgtgctccc ggacggccgg
      601 ttcgcggtgg ccgatgctca ggcggaggag gtgcgcctct tcgacggcca gggtcggcac
      661 ctgcgcacgt tcggcgggaa gggcgcgggc cccggcgagc tccagggcat gcagggtgtg
      721 tacgtcgacc atgagggcct gctgcgggta gccgagcagg ggaacgctcg gctttccgtc
      781 ttccacccgg acacgggatt ggtgacgacc tacccgctgc gcctccacag ctacggtttc
      841 cgcggaccgt ggcccgcggc tgtcgactcg gcgggtcgga cgctggtggc gtcctcgggc
      901 cagtacgggg agggccggta ctggaacatg gtgcgcgtct acgacccgac gatgcaccag
      961 atcgactcga ttccgtaccg cgaatacacc gatgtcgccc gccgagcgac ggggggcggc
     1021 agcgacgatg acttcccggg cgcgtggcgg atcgatctcg gaaacggggg atggacctgg
     1081 gcgcaggtcc cgttctacgc acagccgtac gaggtgctgg caccgaccgg tgagttctgg
     1141 agcagcgccg agggacggcg ggagttggag atcgcccgct ggatgccgcc cggagacacc
     1201 tcgctcgttc tgacgagtcg ccgcccgccg gatccggtca cgccggcaga gcgtgattcc
     1261 gcgatgaccg agctgcggga gacgctggcc cgacggtccc cgagcccgcc gagcctggac
     1321 gcctctcggg ttcccgccac gaagccgccg ctccacgggc tttcgctgga ccgcgccggc
     1381 cgcctgtggg tccgtctcac cgagccgacc gccgactcca ccgtctacga cgtcttcggt
     1441 cgcgacggcc gccacgccga gacggtacgg ctccccttcc gcgtcgaccg ctacgtcccg
     1501 cccctcgtgc gaggggatgt cctgtgggcg gtggtgaccg acgacatgga cgtccagtac
     1561 gtggtccgtg cggcgctgcg ctcgccttcc cgggacacgg gcccctagcg ctggcgagga
     1621 cgaggtcgag atcggtaccg gggccggccg ggtcggcggt ggggcaaccg cggcccgcgg
     1681 gcgcccctcc tcgtcgagaa ttctccgaaa ccccaagggc cacaacgagt ttcggcgctc
     1741 ggaggatccg gcccggacct tgacacctcc ccccgtccgg attagccttt ctagcctctc
     1801 cagccgccgc ctccccgggc gcgtgccgga gggtctgatc ccgcgtagct cagttggtag
     1861 agcaggtggc tgttaaccac cgagtcgcag gttcgagtcc tgccgcggga gctgaaccgc
     1921 cgcctctatt tcgctgcgct gcaacggggt cggggcggcg gttgcacttc gatcgtgttt
     1981 ccgaagggac atttcctcga cgggggggca aggttctccc ccggctcgag gcgcccgtcc
     2041 ccgcggtgcc cccctcgcgg gcgcttcgcg gacgggctcg tgccggcctg tgcgccctac
     2101 cggttgagat gcgccgtcgt gggcgccgtg cctcgcctgt agaagttgaa atcacagaag
     2161 tcgccgccct tggcgagggt gcagggacgt cggaactccg catccactcg atcgaggacc
     2221 accgggtagc cggcgaggtc gtgatcgcag ccgagcctgc cgagctccgg acagccgatc
     2281 gcggtgaaga ggtccacgtt ggcacacgac gtcaccttga tccggagccg gtccccccga
     2341 tcctccacgg actccgtggt ccagg
//
LOCUS       DASQQQ010000347         3907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_52592, whole genome shotgun sequence.
ACCESSION   DASQQQ010000347 DASQQQ010000000
VERSION     DASQQQ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52592"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VE646_12880"
     CDS             <1..293
                     /locus_tag="VE646_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80917.1"
                     /translation="TGTAGPDRLRGTDGTDVICAGRGDDIVRARGGFDLVYGGPGDDT
                     LYGQDGEDLLYGGGGNDIIKAGPSFDGLDGAGGTDVCLPGAGGGYRLRCEGT"
     gene            369..2210
                     /gene="typA"
                     /locus_tag="VE646_12885"
     CDS             369..2210
                     /gene="typA"
                     /locus_tag="VE646_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HZD80918.1"
                     /translation="MNEPRTRDDLRNVAIVAHVDHGKTTLVDAMLWQTGAFRANQEVA
                     ERVMDSMDLEREKGITILAKNTAVRFDGVKVNIVDTPGHADFGGEVERALTMVDGVLL
                     LVDASEGPLPQTRFVLRKALEARLPVILVVNKVDRPDARVREVVDEVEELFLDLDADE
                     HQIDFPILYANARTGRCGTDPGMLGPDLEPLFQALLERIPPPVHQPDHPLQALVTNLD
                     ASPYVGRLALCRVRHGTIHRGQQVAWCRSDGRIERAKVGELYVTEALDRVEADEAGPG
                     EIIAVAGIEEITIGETLADPDDPHPLPVIRIDEPAMSMTIGVNTSPLSGRDGTKMTAR
                     LIKSRLDQEVVGNVSIRVGDTGRPDAWEVQGRGELQLAVLVEMMRREGFELTVGKPQV
                     VTRTIDGRVHEPVERVAIDAPEEYLGVVTQLLALRKGRTVQLVNHGTGWVRMEQLVPA
                     RGLIGFRTEFLTETRGTGQLHHVFDGYEPWHGDLRTRPTGSLVADRIGTTTTFALLNL
                     QERGVLFVGPGEEAYEGMIVGENPRAEDMDVNPTKQKKQSNVRQSTAEELVRLIPPRE
                     LSLEQALEFIRQDECVEVTPTRVRLRKTVLSQHQRAKAARRLSAPRS"
     gene            complement(2191..2652)
                     /locus_tag="VE646_12890"
     CDS             complement(2191..2652)
                     /locus_tag="VE646_12890"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010350709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZD80919.1"
                     /translation="MPEQAPKTVQNFVDLADGQRQWTDPRDGTEKQEPLYDGTIFHRV
                     IEGFMIQGGDPMGTGTGGPGYRFEDEISADGPSFDRPGLLAMANAGPNTNGCQFFVTV
                     APTPWLDGKHTIFGEVTSGFDVVEAISKVDTGSRDRPQRDVVLERVTISER"
     gene            complement(2770..3234)
                     /gene="bcp"
                     /locus_tag="VE646_12895"
     CDS             complement(2770..3234)
                     /gene="bcp"
                     /locus_tag="VE646_12895"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HZD80920.1"
                     /translation="MAELRRGDRAPDFTLEDQHGKEVSLSNSRGHTVLVYFYPAADTP
                     GCTTQSCAVRDARVDLSGLDVDVVGISPDLPDAQTAFDRKYSLGFPLLSDPDHAVAEA
                     WGVWGERVRSTGERVMSIIRSSFLVDEEGRIREAWYKIAPEDTVPRALKVLT"
     gene            3300..3692
                     /locus_tag="VE646_12900"
     CDS             3300..3692
                     /locus_tag="VE646_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004993204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HZD80921.1"
                     /translation="MGYGRTILIDTPLAEAVPRVKEALKEQGFGTLTEIDMRATLKEK
                     LDVDTDPYVILGACNPRLAHRALQAEPDVGLLLPCNVVVRPHEGRTLVSAMDPGVLTE
                     LMDHPDLVPIAEEATTLLDAALDSLSGH"
     gene            complement(3728..>3907)
                     /locus_tag="VE646_12905"
     CDS             complement(3728..>3907)
                     /locus_tag="VE646_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017568290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80922.1"
                     /translation="LPNHVLAVAAPDDDASRETVPLLRDREPTEGLPTAYVCERFACK
                     LPVTDPQALVAQLVV"
BASE COUNT          624 a         1294 c         1419 g          570 t
ORIGIN      
        1 tcaccgggac cgccggcccc gatcggctcc gcgggaccga cgggaccgac gtgatctgcg
       61 ccggccgcgg ggatgacatc gtccgggccc gcggcgggtt cgatctggtg tacggagggc
      121 cgggcgacga caccctgtac ggacaggacg gggaggacct cctctacggc gggggcggga
      181 acgacatcat caaggccggt ccgtccttcg acggcctcga cggggctggg ggtacggacg
      241 tgtgcctgcc cggcgccggg ggcggctacc gcctgcgctg cgagggcacc tgacccgccg
      301 ggctcgaccg gatcgaccca tctatcggtc cagggtcagg tcccgggtcc gccgatacgc
      361 tgggtaccgt gaacgagcct cggacccggg acgatctgcg caacgtggcg atcgtggctc
      421 acgtggacca cggcaagacc acgctggtcg acgcgatgct gtggcagacg ggcgccttcc
      481 gtgcgaacca ggaggtggcc gagcgggtca tggactcgat ggacctggag cgcgagaagg
      541 gcatcacgat cctggccaag aacacggcgg tccgcttcga cggtgtgaag gtcaacatcg
      601 tcgacacgcc agggcacgcg gacttcggcg gcgaggtgga gcgcgccctc accatggtcg
      661 acggggtgct gctgctggtg gacgccagcg aggggcccct gccgcagaca cggttcgtcc
      721 tgcgcaaggc gctcgaggcc cggttgccgg tgatcctggt ggtgaacaag gtggatcggc
      781 cggacgcccg cgtgcgcgag gtggtggacg aggtcgagga gctgttcctg gacctggacg
      841 cggacgagca ccagatcgac ttccccatcc tgtacgcgaa cgcgcggacc ggtcgatgcg
      901 ggaccgaccc gggcatgctc ggaccggacc tcgagccgct gttccaggcg ctgctggagc
      961 ggatcccccc gcccgtccat cagcccgatc acccgctcca ggccctggtc acgaacctcg
     1021 atgcctcgcc ctacgtcggc aggctcgccc tgtgccgcgt gcgccacggc acgatccacc
     1081 ggggccagca ggtggcctgg tgccggtccg acggtcgcat cgagcgggcg aaggtggggg
     1141 agctctacgt gacggaggcc ctggaccggg tggaagcgga cgaggccggg cccggcgaga
     1201 tcatcgcggt cgccggcatc gaggagatca cgatcggcga gacgctggcc gacccggacg
     1261 acccgcaccc gctcccggtg atccggatcg acgagccggc gatgtcgatg acgatcggcg
     1321 tgaacacctc gccgctgtcg ggacgggatg ggaccaagat gacggcccgc ctgatcaaga
     1381 gccgcttgga tcaggaggtg gtcggcaacg tctcgatccg ggtcggcgac accggtcgac
     1441 ccgacgcctg ggaggtccag gggcggggcg agctccagct ggcggtcctg gtcgagatga
     1501 tgcgccgcga ggggttcgag ctcacggtgg gcaagcccca ggtcgtgacc cggacgatcg
     1561 acggccgcgt gcacgagccc gtggaacggg tggcgatcga cgcgcccgag gagtacctgg
     1621 gcgtggtcac ccagctgctg gcgctccgca agggccggac ggtgcagctg gtcaaccacg
     1681 ggaccggttg ggtgcggatg gagcagctgg tgccggcccg agggttgatc ggcttccgca
     1741 ccgagttcct gacggagacc cgtggcaccg ggcagctgca tcacgtgttc gacggctacg
     1801 agccgtggca cggcgacctg cggactcgcc ccaccggctc cctggtggcg gaccggatcg
     1861 gcaccaccac caccttcgcc ctgctgaacc tccaggagcg cggggtgctg ttcgtggggc
     1921 ccggcgagga ggcctacgaa ggcatgatcg tgggcgagaa cccgcgcgca gaggacatgg
     1981 acgtcaaccc caccaagcag aagaagcagt cgaacgtccg tcagtccacg gccgaggagc
     2041 tggtccggct catcccccct cgggagctgt cgctggagca ggccctggag ttcatccggc
     2101 aggatgaatg cgtcgaggtc acgcccaccc gggtgcgcct gcgcaagacc gtcctgtcgc
     2161 agcatcagcg ggccaaggcg gcccgccggc tcagcgctcc gagatcgtga cgcgctccag
     2221 caccacgtcc cgctggggac ggtcgcggga tccggtgtcc accttggaga tcgcctcgac
     2281 cacgtcgaac cccgacgtca cctcgccgaa gatggtgtgc ttgccgtcga gccacggggt
     2341 gggggccacc gtcacgaaga attgacagcc gttcgtgttc ggtcccgcgt tggccatggc
     2401 cagcaggccc gggcggtcga aagatggtcc gtcagccgag atctcgtcct cgaaccggta
     2461 gccggggccg cccgtcccgg tccccatggg gtcgccgccc tggatcatga acccttcgat
     2521 cacccggtgg aagatcgtcc cgtcgtacaa gggctcctgc ttctcggtgc cgtcccgggg
     2581 atcggtccat tgccgttggc cgtccgccag gtcgacgaag ttctgcacgg tcttcggtgc
     2641 ctgctcgggc atcagctcca ccgtgaagga gcccaaggag gtgtggaagg tcgccgtggt
     2701 cacgtggaca ccacctcttc ctctcggcga tgacctcagt ctatgcgagt gaacgaagga
     2761 gaggagcggc taggtcagca ccttgagcgc gcgagggacg gtgtcctcgg gagcgatctt
     2821 gtaccaggct tcgcggattc gcccctcctc gtccaccagg aaggaggagc ggatgatgct
     2881 catgacccgc tcgcccgtgg atcgcacgcg ctccccccag acgccccagg cctcggccac
     2941 cgcgtggtcg gggtcggaca ggagggggaa tcccagcgag tacttccgat cgaaggcggt
     3001 ctgggcatcg gggaggtcgg ggctgatgcc cacgacgtcc acgtccagcc ccgacaggtc
     3061 caccctggca tcgcgcacgg cgcaggactg ggtggtgcac ccgggcgtgt ccgccgccgg
     3121 atagaagtag acgaggaccg tgtgccctcg ggagttcgac agcgagacct ccttgccgtg
     3181 ttgatcttcg agggtgaagt cgggggcccg gtcgccccgt cgaagctcgg ccatatgacc
     3241 agggtacccc ggtccggtcg atgactagtg tcggtgccga ccggagcagg aggtcacaga
     3301 tgggatacgg acgcacgatc ctgatcgata caccgctggc cgaagccgtc ccccgcgtga
     3361 aggaggcgct gaaggagcag gggttcggca cgctgaccga aatcgacatg cgggccacgc
     3421 tcaaggagaa gctcgacgtc gacacggacc cgtacgtgat cctgggggcc tgcaacccgc
     3481 ggctggctca tcgggccctc caggcggagc ccgacgtcgg gctcctgttg ccgtgcaacg
     3541 tggtggtgcg cccacacgag ggacgcaccc tggtctcggc catggacccg ggtgtgctga
     3601 ccgagctcat ggaccatccc gatctggtgc cgatcgcgga ggaggccacc acgctgctcg
     3661 acgcggctct cgactcgctg agtggccact gaccccggga cctgcgcgga cgacgtctgg
     3721 acctgactca gacgaccagc tgtgcgacca gagcctgggg gtcggtcacg gggagcttgc
     3781 aggcgaagcg ctcgcagacg taggcggtgg gcaggccctc ggtcggctcg cggtccctca
     3841 gcagcggcac cgtctcccgc gaggcatcgt cgtccggtgc ggccacggcg agcacgtggt
     3901 tggggag
//
LOCUS       DASQQQ010000348         6186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_24880, whole genome shotgun sequence.
ACCESSION   DASQQQ010000348 DASQQQ010000000
VERSION     DASQQQ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24880"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..715)
                     /locus_tag="VE646_12910"
     CDS             complement(83..715)
                     /locus_tag="VE646_12910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZD80923.1"
                     /translation="MVERQLLLIDANGGARETARALEREGFVVVHEHDVDPAVARLRR
                     QAFDLAVIDAVLPGGDGFAACRMLRQADLLPVVMIAPVSGGTAVVNALEAGADDCVTK
                     PFEMPELVARIRAVLHRHARTEPFIRAGRVEVDVEGFRAWKDGEELDLTATEFRLLSV
                     LAARRGRVCSKQLLLSRVWHDGYGGVSRAIGRRSKAVCWMTALRSPIGSS"
     gene            1528..2211
                     /locus_tag="VE646_12915"
     CDS             1528..2211
                     /locus_tag="VE646_12915"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HZD80924.1"
                     /translation="MPTEGQLVLRLLLAAALAGALGVERELTGQPAGFRTHVLVGLGA
                     ALFAVVSAFGFQPILGLTTEPGARVDLSRVASQIVVGIGFLGGGAILKYGLSIRGLTT
                     AASLWITAAVGTAVGIGALLIGTATTGIALVALVGLRPVRRAIQRRAPLQTEFVVEGD
                     GRMDVAKMLGAFRPDQDLVRQVKVEDEYDRRQIRILVHLASDTEPARVAGTMTDDPHV
                     ESVDWTSES"
     gene            complement(2213..2473)
                     /locus_tag="VE646_12920"
     CDS             complement(2213..2473)
                     /locus_tag="VE646_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HZD80925.1"
                     /translation="MPGGKGPGPSVKDEQQYEALRGKGMSKEKAARIANSSRSGTGKR
                     GGSSQKYEEWTKQDLYEKAKEVGIEGRSSMSKKELIGALRNH"
     gene            complement(2490..3002)
                     /locus_tag="VE646_12925"
     CDS             complement(2490..3002)
                     /locus_tag="VE646_12925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HZD80926.1"
                     /translation="MDEALDRETGSIQRGSPVESRAERFRQYEGPGAGRIREVDPLPV
                     PVAELLRGRNFAHLATLMPDGSPHVAPVWVDLEDGLIRVNTVEGRVKTRNLRRDPRVA
                     LSVDDHEDPYRMASIRGEVVEVTIEGADEHIDALAGKYLGVDRYPNRRRGERRVIVKI
                     RPDRVATMGA"
     gene            3177..4286
                     /locus_tag="VE646_12930"
     CDS             3177..4286
                     /locus_tag="VE646_12930"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZD80927.1"
                     /translation="MLGVDVKVDASWALIALLIGYSFFFQFRLVYPSLSTWASVGLAV
                     GETLVFFGSVLAHELAHAVVAKARGIPVRDITLFLFGGVTRARVESHGPGEEFLIAVV
                     GPVTSLVLGGALWAVAILVPGLPDAVAGAVGYLGLVNLALGVFNLVPGFPLDGGRLLR
                     STLWKTSGDLGRATRIAAAVGQVVGWLMVAGGLALVATVSLIGGIWIAAIGWFLVQAA
                     RASYADLRIRTMLEVVDAEEVMTETVVTVPTEITIQEAVDRYLRSVDVDAVPVQQDGR
                     TVGILTVEAVRRLGREHWDDRRVGEIMAPLVREPRVDAKTRMDRVVRKLEDVEGQRVF
                     VARGAQVVGVITSGDISRWVTGHRGLEREHDRKAA"
     gene            4310..5278
                     /locus_tag="VE646_12935"
     CDS             4310..5278
                     /locus_tag="VE646_12935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006108782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HZD80928.1"
                     /translation="MELGYMLSSEEHPPGDLIAAAAAAEEAGFTFAMISDHYNPWIDR
                     QGQSPFVWAVIGGLAQVTERIRIGTGVTCPTIRIHPAIVAQAAATAACMLPGRFFLGV
                     GSGERVNEHVVDGPWPAPDARLEMLEEAIGIIRDLWEGDLTTFRGTHFTVEQGRLYTL
                     PDMPPPLYVAATGSRSVSVAAKAGDGLITTSPDAQLVRSFHDAGGRGPRLGKLTVCWD
                     QDPERARRTAHEIWPNIAIPGPVGAELPLPRDFEQITSLLTSEEVCSRVVCGDDPEEH
                     LAAIRGYADAGFDHLVVHQVGPRQEGFLHFYRDRILPEAVDLEAAA"
     gene            5310..5639
                     /locus_tag="VE646_12940"
     CDS             5310..5639
                     /locus_tag="VE646_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80929.1"
                     /translation="MRPELAQRQRVFRRRLRIRLAIGGTIGAVVLGIAGAVVGSVFFG
                     TGSGVMWGTFAGAVAVGIAFGLFLGGVGSLDAPRPGHEPLDTNPPEDDLGTRSTAAVP
                     REDRDGG"
     gene            complement(5677..6036)
                     /locus_tag="VE646_12945"
     CDS             complement(5677..6036)
                     /locus_tag="VE646_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012689061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80930.1"
                     /translation="MPHEGPSRDEELAREEPEPLQEEPRGSDEFGVTAEEQRRGEPLD
                     ERLEREEPEGRGSAPREADQLVDERTNAPDVAPELVADEAGETDRSRPAEEEAMRVEE
                     GEAPGGVDRPSDGYVNE"
BASE COUNT          858 a         2211 c         2156 g          961 t
ORIGIN      
        1 tggtcgttaa tggtccattc cgtctcgccg gtcgcatcta cctcaccatt caggctgaag
       61 tggttggcct gcgagctgtg gatcaggatg atccaatcgg cgatctgaga gccgtcatcc
      121 agcagacggc cttcgacctg cgcccaatcg ccctggagac ccccccgtag ccgtcgtgcc
      181 agacccgtga gagcaggagt tgcttgctgc agacccggcc gcgtctggcc gccaggacgc
      241 tcagcaaccg gaactccgtg gcggtgagat cgagctcctc cccatccttc caggcccgga
      301 acccctccac atcgacctcc acacggcccg ctcggatgaa gggctcggtc cgggcatgac
      361 ggtgcaggac ggcacggatc cgggccacga gctccggcat ctcgaagggc ttcgtgacgc
      421 agtcgtccgc ccccgcttcc agggcgttca ccacagccgt cccgccggag accggcgcga
      481 tcatgaccac cggcagcagg tcggcctgcc gcaacatccg gcaggcggcg aacccgtcgc
      541 caccgggcag gaccgcatcg atcaccgcga gatcgaacgc ttgccggcgc agccgcgcca
      601 ccgcggggtc cacgtcgtgc tcgtgcacca ccacgaagcc ctcgcgttcc agcgcccgcg
      661 ccgtctcccg ggcgccgccg ttggcgtcga tcagcaggag ctggcgctcg accatctcgc
      721 tcaccggccc ccgggaccct caccgacccg cgagctcgcg agagcgacga tctccagatc
      781 cggggagaga cgtgtggagg gaaccggtgg agcccgcctg ccgttcgagc cgcccgtggt
      841 gatccggtcg atagccaccg cggggccgct cacccagatg gcacgcgccc gtgccaccgt
      901 gctcggcagg agcgtcggag tgaccccctc ccatccgatg ggtccgcgga gggagccggc
      961 gcgggaagtc cccctcgccg tggaggtggt cacctcgtcc gacggcgttc cggccggctc
     1021 cccgcgatga ctcccgccac ccagggtcgt gacggtcgat ccataccgcg cgaaggtgct
     1081 cgtggcgttc gaggtgggag cggtcgtccg ggtggggaac gtggtggttc cgtatcgggc
     1141 cagagcgacc gagacaacca gcaggaaact cagcggcaag aggaggcgtg tgcgaagtcc
     1201 cgacccctcc gccggccgac accccgtgat cgctcccggg cgtcgagcac gcgccatcgc
     1261 gccatcgaca ccggggaccc cccaaccgat cgtacgtccc cctcggtgct cgtcgttcgc
     1321 ggacccgggc gggcgacgca tccgcgggcc gaggattccc cccctccgcc cggcccaaac
     1381 atccggaccg ccgaacatcc ggatcacagg tcgtcgcggg tccggtgcgg gaccacgacg
     1441 cggagagacg acatactatc cgcgtagtcc ccgggccggt ccgcaggaga cgagcacggg
     1501 gaccaggcgg atggaggtca tcgggtcatg ccgaccgagg ggcagctggt ccttcgtctg
     1561 ctgctggcgg ccgccctggc gggggcgctg ggcgtcgagc gtgagctcac tgggcagccg
     1621 gcggggttcc gcacccacgt gttggtcggg ttgggggcgg cgctgttcgc cgtggtgtcc
     1681 gccttcggct tccagcccat cctggggctc accaccgaac ccggagcccg ggttgacctg
     1741 tcccgggtcg cctcgcagat cgtggtgggg atcgggttcc tgggcggagg ggcgatcctg
     1801 aagtacgggc tcagcatccg ggggctcacc acggccgcca gcctgtggat cacggccgcc
     1861 gtaggcacgg cggtaggcat cggggcactc ctgatcggaa cggccaccac cggcatcgcc
     1921 ctggtcgcgc tggtggggtt gcgaccggtc cggcgggcga tccaacgacg agcccccctc
     1981 cagaccgagt tcgtagtcga gggggacggc aggatggacg tagcgaagat gctcggggcg
     2041 ttccgacccg atcaggacct ggtccgccag gtgaaggtgg aggacgagta cgatcgccgg
     2101 cagatcagga tcctggtcca cctggcctcg gacacggagc cggcccgcgt ggccgggacc
     2161 atgacggacg acccccacgt ggagtcggtg gactggacat ccgagtcgta gatcagtggt
     2221 tccggagcgc gccgatcagc tccttcttcg acatgctcga gcgtccctcg atcccgacct
     2281 ccttggcctt ctcgtagagg tcctgcttgg tccactcctc gtacttctgc gaggatcccc
     2341 cgcgcttgcc ggtcccgctg cgcgaggagt tcgcgatgcg ggcggccttc tccttgctca
     2401 tgcccttgcc gcggagggct tcgtactgct gctcgtcctt cacgctcggt cccggaccct
     2461 tgcctcctgg catcgccgtg cctcctccct catgcaccca tcgtggcaac gcgatcgggg
     2521 cggatcttga cgatcacccg ccgctcgccg cgccttcggt tggggtaccg atccaccccc
     2581 aggtacttcc cggctagagc gtcgatgtgc tcgtccgctc cctcgatcgt gacctccacg
     2641 acctcgcctc ggatcgacgc catccggtac gggtcctcgt ggtcgtcgac cgacagcgcc
     2701 acccggggat cacgccggag gttgcgcgtc ttcacccgcc cctccaccgt gttcacccgg
     2761 atcagcccat cctccagatc cacccaaacc ggggccacgt gcggcgaacc atcgggcatc
     2821 agggtggcaa ggtgggcgaa gttccggccc cgcagcagct cggccaccgg gacgggcagg
     2881 gggtcgacct ccctgatgcg cccggcgccc ggcccctcgt actgacggaa ccgctcggcc
     2941 cgggactcca ccggggaccc gcgttggatc gacccggtct cccggtcgag cgcctcgtcc
     3001 atccgcgctc tatgcgcgtc gctcctccgc tgcatacctg gtgggttccc gcacctgcgc
     3061 aaccgctaaa cccccgcgcg agggaaccct cggcgtcggc catcagggca ggcgtcccgc
     3121 ctgccgtgcg cgggtatgcc ataggtgtgt tcgggaagtc gtggcggatc gggcgcatgc
     3181 tcggcgtcga cgtcaaggtc gacgcctcgt gggccctgat cgccctcctg atcggctaca
     3241 gcttcttctt ccagttccgc ttggtctacc cgagcctcag cacctgggcc tcggtgggtc
     3301 tggccgtggg cgagacgctg gtcttcttcg gctcggtgct cgctcacgag ctcgcgcacg
     3361 cggtggtggc aaaggcccga ggcatcccgg ttcgggacat cacgctcttc ctgttcggcg
     3421 gtgtgaccag ggcccgcgtc gagtcgcacg gaccgggcga ggagttcctg atcgcggtgg
     3481 tgggacctgt caccagcctg gtcctcggcg gcgccctctg ggcggtggcg atcctggtcc
     3541 ccggcctccc cgacgccgtg gccggagcgg tcggctacct cggcctggtc aacctggccc
     3601 tgggcgtctt caacctggtg ccgggattcc ctttggacgg cgggcgcctg ctccgatcga
     3661 cgctgtggaa gacgagcggt gacctgggtc gagcgacccg catcgcggcg gccgtcggcc
     3721 aggtcgtggg ctggctcatg gtggccgggg gcctggcgct ggtggcgacc gtatcgttga
     3781 tcggagggat ctggatcgcg gcgatcggct ggtttctggt gcaggccgcc cgggcctcgt
     3841 acgcggacct gcggatccgc acgatgctcg aggtggtgga cgcggaggag gtgatgaccg
     3901 agaccgtcgt caccgtcccg acggagatca ccatccaaga ggcggtcgac cggtacctga
     3961 ggtccgtcga tgtggacgcg gttcccgtgc aacaggacgg acgcacggtc gggatcctca
     4021 cggtagaggc ggtgcgccga ctgggtcgcg agcactggga cgaccgtcgg gtgggagaga
     4081 tcatggctcc gctcgtccgc gagccgcgcg tcgatgccaa gacccgcatg gatcgggttg
     4141 tccgcaagct cgaggacgtc gagggccagc gcgtgttcgt cgcccgggga gcccaggtcg
     4201 tgggggtcat cacctccggc gacatcagtc ggtgggtcac aggccatcgc gggctcgagc
     4261 gggagcacga ccggaaggcc gcctgaggac cggaggaagg agagggtcca tggagctcgg
     4321 ctacatgttg tccagcgagg agcacccacc gggggacctg atcgccgcgg ccgccgccgc
     4381 agaggaggcc gggttcacct tcgcgatgat ctccgatcac tacaacccgt ggatcgaccg
     4441 acaagggcag agccccttcg tgtgggcggt gatcggcggc ctggcccagg tcaccgagcg
     4501 gatcaggatc gggaccgggg tgacctgccc cacgattcgg atccacccgg ccatcgtggc
     4561 gcaggcggcg gccacggccg cctgcatgct gcccggccgc ttcttcctgg gcgtgggcag
     4621 cggcgagcgg gtcaacgagc acgtggtgga cggaccctgg ccggcacccg atgcccggct
     4681 ggagatgctt gaggaggcga tcgggatcat ccgcgacctg tgggaggggg atctcaccac
     4741 gttccgaggg acgcacttca ccgtggagca gggccggctc tacacgctcc cggacatgcc
     4801 tccccccctg tacgtggccg ccaccggctc tcgctccgtc tccgtggcgg cgaaggccgg
     4861 cgacgggctc atcaccacgt cccccgacgc ccagctcgtg cgctccttcc acgacgccgg
     4921 cggcaggggc ccgcggctgg gcaagctcac ggtctgctgg gaccaggatc ccgagcgggc
     4981 gcggcgcacc gcgcatgaga tctggcccaa catcgcgatc cccgggccgg tcggagccga
     5041 gctaccgctg cccagggact tcgagcagat caccagcctg ttgacctcgg aggaggtgtg
     5101 ctcgcgggtg gtgtgcggcg atgacccgga ggaacacttg gccgcgatcc gtggatacgc
     5161 ggacgccggg ttcgatcacc tggtcgtaca tcaggtcggc ccgcggcagg agggcttcct
     5221 ccacttctac cgagaccgga tcctgcccga ggcggttgac ctggaggcgg cggcctgagc
     5281 gggcccgaac gggaaggagg gggatcacca tgagaccaga gcttgcccaa cgacagcggg
     5341 tgttccgacg tcggctgcgc atccgcctgg cgatcggcgg cacgatcggg gccgtggtcc
     5401 tcgggatcgc cggggcggtg gtggggtccg tgttcttcgg caccgggagc ggggtgatgt
     5461 ggggaacgtt cgccggagcg gtggccgtcg gcatcgcctt cggcttattc ctcggtgggg
     5521 tggggtcgct ggatgccccc cggcccggcc acgaacccct cgatacgaac ccgcccgagg
     5581 acgatctggg cacccgatcc acggcggcgg tcccccgaga ggatcgggac gggggatgag
     5641 cccggccggc gggtccggtc ggggcggcgt gggccgctac tcgttcacgt agccgtccga
     5701 gggacggtcc acgccgcccg gcgcctcgcc ctcctccacc cgcatggcct cctcctcggc
     5761 gggacgggac cgatcggtct cacccgcctc gtcggccacc agctcgggcg ccacgtccgg
     5821 agcgttggtt cgctcgtcga ccagctggtc cgcttcgcgg ggcgccgacc ctcggccctc
     5881 gggctcctcg cgctccagac gctcgtccaa cggctcgccg cggcgctgct cctccgcggt
     5941 cacgccgaac tcgtccgaac ctcgcggctc ctcctgcagg ggctccggct cctcgcgggc
     6001 cagctcctcg tctcggctag ggccttcgtg gggcatgcac gaccgatacc cccacggcct
     6061 cccggggaaa cggcgatcgg tttcgctccc gaaaggggcg ggtagccgaa ccccatgatg
     6121 cgcgagcccg gccccacccc ccagccaccg ccggaccccg ctccggtgcc gcagccgccg
     6181 cagccg
//
LOCUS       DASQQQ010000349         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_52758, whole genome shotgun sequence.
ACCESSION   DASQQQ010000349 DASQQQ010000000
VERSION     DASQQQ010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52758"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..976
                     /locus_tag="VE646_12950"
     CDS             26..976
                     /locus_tag="VE646_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZD80931.1"
                     /translation="MDLGLAELRRRAAAAVASLAECRACPRDCGVDRLAGKWSACKTG
                     RWAVVSSAFPHFGEEDCLRGWNGSGTIFFAHCNLRCQFCQNFDISQGVRPGPVAAPGA
                     PPERIAHLMLDLQTRGCHNINFVTPEHVVPQVLEALVPAVERGLRIPLVYNTSAYDSL
                     ASLRHLDGVVDIYMPDIKMWSRERARTYLKAEDYPDAARAAVQEMHRQVGDLVLAGDG
                     LARRGLLVRHLVMPGQLDETRRILEWIARELGPDTWVNLMDQYHPAGKVGPDHLPELD
                     RRLRAGEFAEALLAARRAGLRRLDERRPHPRLAARPFLRL"
     gene            complement(1051..1812)
                     /gene="pgl"
                     /locus_tag="VE646_12955"
     CDS             complement(1051..1812)
                     /gene="pgl"
                     /locus_tag="VE646_12955"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HZD80932.1"
                     /translation="MADSVSRPAPPGHEDGPRPGAVRIEEGEAWTETAGSAVAGPLRD
                     AVTSDGKASLALAGGSTPEPVYRWLARSGPLPWERVDLFFGDERCVPPDDPASNYRMV
                     RETLLEPLGADPQRVHRMQAERPDRDAAADDYAAVLPPRLTVLLLGIGPEGHTASLFP
                     GSPALDERRRRVVPVSTPAKPPERLTVTPPVLEAARHVVVLARGDRKADAVRRALEGS
                     WSPTACPAQLARRGTWILDPAAAALLTGRDGRPVS"
     gene            complement(1797..>2608)
                     /locus_tag="VE646_12960"
     CDS             complement(1797..>2608)
                     /locus_tag="VE646_12960"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZD80933.1"
                     /translation="TRGSFYEGTGVVRDMVQNHLLQLLCMATIEPPVSFDAASLRDET
                     VKVLQAVQVTPFDPRRDAVRGQYGPGNVDGESVAGYREEEDVAPESGTATYAALRLTM
                     DNWRWTGVPFYLRTGKRMRRKLTEIAIQFRPTPHLMFPVERRHDLGGNVLAFRLQPEE
                     GILQEFIAKQPGPDIALRPVTMNFRYATAFGVEEPPRAYAWLIMDVMQGDQRLFARSD
                     WIDEAWAVVDPLVEHWESTEPEGFPDYAAGSWGPDAASRLLARSGRQWRTL"
BASE COUNT          332 a          981 c          934 g          361 t
ORIGIN      
        1 gtccgcgccc gtccggcccg cctacctgga cctgggtctc gccgagctgc gccgccgcgc
       61 cgcagccgcc gtggcctccc tggcggagtg ccgggcgtgc ccccgggact gtggggtgga
      121 ccggctggca ggaaagtggt cggcgtgcaa gacgggccgc tgggccgtcg tctccagcgc
      181 ctttccccac ttcggcgaag aggattgcct ccgggggtgg aacggcagcg gcacgatctt
      241 cttcgcccac tgcaacctgc gctgccagtt ctgccagaac ttcgacatct cccagggtgt
      301 ccgccccgga cccgtcgcgg ctcccggcgc gccgcccgag cgcatcgccc acctcatgct
      361 cgacctccag acccggggct gccacaacat caacttcgtg acgccggagc acgtggtgcc
      421 ccaggtcctc gaggccctgg tccccgcggt ggagcggggc ctccgcatcc ccctggtcta
      481 caacaccagc gcctacgact ccctggcgag cctccgccac ctggacggcg tcgtcgacat
      541 ctacatgccc gacatcaaga tgtggagccg ggagcgtgcc cggacctacc tcaaggccga
      601 ggactacccc gacgcggccc gcgcggcggt gcaggagatg caccgtcagg tgggcgacct
      661 ggtgctggct ggcgacggtc tcgcccgtcg ggggctcctg gtccgccacc tggtcatgcc
      721 cgggcagctc gacgagaccc ggcggatcct ggagtggatc gcccgggagc tcggcccgga
      781 cacctgggtc aacctcatgg accagtacca tccggcgggg aaggtggggc cggaccacct
      841 gccggagctc gaccgtcgcc tccgcgccgg cgagttcgcc gaagcgctgc tcgcggcccg
      901 gcgggcgggc ctccggcgcc tggacgagcg gcggccccac ccgaggctgg ctgctcgacc
      961 tttcctgcgg ctctgacgcg gagtccgccc gcgggcgccg gaaggtctcc gcccccgtgc
     1021 cggaggccga gcggccctcg ccggcggacg tcacgacacc ggcctcccgt cccgtccggt
     1081 cagcagggcg gcggccgccg gatccaggat ccacgtgccc cttcgggcaa gctgggcggg
     1141 gcaggcggtg gggctccagg agccttccag cgcccgccgg accgcgtcgg ccttgcggtc
     1201 cccccgggcc aggaccacga cgtggcgggc cgcctccagc accggcggcg tgacggtgag
     1261 tcgctccggc ggtttggccg gggtcgagac gggcacgacc cggcggcgac gctcgtccag
     1321 cgcgggcgaa ccggggaaga gcgatgcggt gtggccttcc ggtccgatcc ccagcagcag
     1381 caccgtgagc cgcggcggga ggaccgcggc gtagtcgtcg gccgcggcgt cccggtccgg
     1441 tcgctcggcc tgcatgcggt ggacccgctg cgggtccgct cccaggggct cgaggagcgt
     1501 ttcccgcacc atccggtagt tgctcgcggg atcgtccggg ggcacgcagc gctcgtcgcc
     1561 gaagaagagg tcgacccgct cccaggggag cgggccggag cgcgccagcc agcggtagac
     1621 cggctcgggg gtggagcctc cggccagggc gagagaggcc ttgccgtccg acgtcaccgc
     1681 atcgcggagc gggccggcca ccgccgaccc cgccgtttcg gtccacgcct cgccttcctc
     1741 gatgcgaacg gcgccgggtc gcggcccgtc ctcgtggccc gggggggcag ggcggctcac
     1801 agagtccgcc actggcggcc gctgcgcgcc aggagccggc tcgccgcgtc ggggccccag
     1861 ctcccggccg catagtcggg aaagccctcc ggctccgtcg attcccagtg ctccaccagg
     1921 ggatccacca cggcccaggc ctcgtcgatc cagtcgctcc gggcgaagag gcgctggtcc
     1981 ccctgcatga cgtccatgat cagccacgcg taggctcgcg gcggttcctc cacaccgaag
     2041 gccgtggcgt agcggaagtt catggtgacc gggcgcaggg cgatgtccgg acccggctgc
     2101 ttggcgatga actcctgcag gatcccttcc tcgggctgga ggcggaacgc cagcacgttc
     2161 ccgccgaggt cgtgccggcg ctctacgggg aacatgagat ggggcgtggg gcggaactgg
     2221 atggcgatct cggtgagctt ccgcctcatg cgcttgcccg tccgcagata gaacggcacc
     2281 cccgtccagc gccagttgtc catcgtgagg cggagggccg cgtaggtcgc cgtccccgac
     2341 tccggggcca cgtcctcttc ctcccggtag cccgccacgc tctccccgtc cacgttcccc
     2401 ggcccgtact gtccgcgcac ggcgtcccgc cggggatcga agggggtgac ctggaccgcc
     2461 tggaggacct tgacggtctc gtcccggagc gaggccgcgt cgaagctcac cggaggctcg
     2521 atcgtggcca tgcacaggag ctggagcagg tggttctgga ccatgtcccg taccaccccc
     2581 gtcccctcgt agaacgagcc gcgggtcc
//
LOCUS       DASQQQ010000350         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_136817, whole genome shotgun sequence.
ACCESSION   DASQQQ010000350 DASQQQ010000000
VERSION     DASQQQ010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136817"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="VE646_12965"
     CDS             <1..1502
                     /locus_tag="VE646_12965"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZD80934.1"
                     /translation="PTRPGSGLYRGGFAASVPLGRGRRTDRPPPVTPFEPSIDPACPG
                     SAGDSTRASTPMGSATCATIEVVVPRGARRVATEDGSTPLLPGTAAELPVADSARVRF
                     PLDLWIVDGPGPVAVLHESPSEVGRDGLVVGRPEPAATYAWLWSDGPHALVTGRRNDE
                     VRLALDAGTAAWVPFGDLTWPDLDPAGETEPTRVGTVQLYDGPDRLQVQVAVGRPVPY
                     RVAIDGHRLVLTLYGAFSDTDWLRYGPHPTYVRRAGWSQPTGDRYVLEMELATTPWGY
                     RARYRAGALILEVRKPPAIDPEHPLEGRTIVLDPGHPPAGATGPSRLYEGDANLAVAF
                     RARRLLEAAGATVFLTRADRRAVRLYDRPALAERLNADVLVSIHNNALPDGVNPFSNH
                     GTSVYYFQDQEADLARALQQSLLATLGLPDLGFGRGNLALTRPTWMPSVLTEGAFMMI
                     PEEEAGLRSPAFLEAYALGVVDGIRAFLRERALEPGARPEGASSPPRKP"
     gene            1499..>2214
                     /locus_tag="VE646_12970"
     CDS             1499..>2214
                     /locus_tag="VE646_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80935.1"
                     /translation="MTRGLLAAALTLLAAMPQARAQVPNARYRQFETEHFRVVFPREM
                     EPFARRAAAGAEWAYAALARDLVGPPPGRIALVLSDGSDAPNASATPIPRNRVVLDIA
                     PQIVSRQLNNYPDWVDLTLAHELTHVFHLDRAEGVWRVPRALFGRFPLFFPAFYQPVW
                     MIEGLATYEESRLTGAGRADGSYFRTILAAQARAGEFHPVDAGNGIAPLWPAGQTPYA
                     YGSHYLRSLARAHGDSTIPEL"
BASE COUNT          281 a          845 c          767 g          321 t
ORIGIN      
        1 cgcccactcg gccgggcagt gggctctacc gcggcggctt cgccgcctcg gtgcccctgg
       61 gccgcgggcg acggaccgac cggccgccgc ccgtcacgcc gttcgaaccg tcgatcgacc
      121 cggcctgccc gggatccgcc ggcgattcca cgcgcgcctc cacgccgatg ggctccgcga
      181 cgtgcgcgac catcgaggtc gtagtcccgc gcggagcgcg ccgggtggcc acggaggacg
      241 gctcgactcc tctgctgcca gggacggcag cggagcttcc ggtcgccgac agcgcccggg
      301 ttcggttccc tctcgacctc tggatcgtgg acgggcccgg gcccgtcgcc gtgctgcacg
      361 aatcgccgtc ggaggtcggg cgcgatggcc tggtcgtggg ccgcccggag ccggcggcga
      421 cctacgcgtg gctgtggagc gacggccccc acgccctcgt caccggccga cggaacgacg
      481 aggtccggct ggcactggac gccgggaccg cggcctgggt cccgttcggc gatctgacct
      541 ggccggacct cgatcccgcc ggcgagacgg agccgacccg ggtgggaacg gtgcagctgt
      601 acgacgggcc cgaccggctc caggtgcagg tcgcggtcgg ccggcccgta ccgtatcgcg
      661 tggccatcga cgggcatcgt ctcgttctca ccctctacgg tgcgttctct gacaccgact
      721 ggctccgcta cggacctcac ccgacgtacg tgcgccgggc ggggtggtcg cagcccaccg
      781 gcgaccgtta cgtgctggag atggagctcg ccaccacgcc gtggggctac cgcgcgcggt
      841 atcgggccgg cgccctaatc ctcgaagtcc ggaagccccc ggcgatcgat cccgagcacc
      901 ctctggaagg ccgtaccatc gtcctcgatc cgggtcaccc accggcgggg gccacgggcc
      961 cgtcgcgact gtacgagggc gacgccaacc tcgccgtcgc gttccgggcg cggcgcctgc
     1021 tcgaggccgc gggcgccacc gtgttcctga ctcgggcgga ccgacgggcc gtccgcctgt
     1081 acgaccggcc ggctctggct gagcggctga atgccgacgt cctggtctcc atccacaaca
     1141 acgcgctccc cgatggcgtg aacccgttct cgaaccacgg gacgagcgtg tactacttcc
     1201 aggaccagga ggcggacctg gcccgggcgc tgcagcaaag cctgttggcc actctggggc
     1261 tgcccgatct gggcttcggc cgtggcaacc tggcgttgac ccggcccacc tggatgccgt
     1321 ccgtgctcac ggagggcgcg ttcatgatga tcccggagga ggaggccggc ctccgaagcc
     1381 cggccttcct cgaggcgtac gcgctaggtg tggtggacgg gatccgggcc ttcctgcgcg
     1441 agcgtgccct ggagcctgga gcccggccgg agggcgcgtc gtctccgccg cggaaaccgt
     1501 gacccgcgga ctgctcgccg cggctctgac gctcctggcg gccatgccgc aggcacgcgc
     1561 gcaggtcccg aacgcccgct atcgccagtt cgagacggag cacttcaggg tcgtctttcc
     1621 ccgggagatg gagcccttcg cccgccgcgc ggcggccggc gccgagtggg cctacgcggc
     1681 actcgcccgg gatctcgtcg ggccgccgcc gggccgcatc gccctggtgc tcagcgacgg
     1741 cagcgacgcc ccgaacgcca gcgccacgcc gataccgcgg aaccgtgtcg tgctggatat
     1801 cgcaccgcag atcgtctcgc ggcagctcaa caactacccg gactgggtgg acctcaccct
     1861 cgcccacgag ctcacccacg tcttccacct ggatcgagcc gagggagtct ggcgcgtgcc
     1921 ccgggcgctg ttcggtcgct tccccctctt cttccccgcg ttctaccagc cggtgtggat
     1981 gatcgaggga ttggccacat acgaggagtc gcggctcacc ggggcgggcc gggccgacgg
     2041 gtcttacttc cgcacgatcc tggccgccca ggcccgggcc ggcgagttcc atcccgtgga
     2101 cgccggcaac ggaatcgctc ccctctggcc ggccggtcag acgccctacg cctatggcag
     2161 ccactacctg cgctccctgg cccgggccca cggcgactcc acgatccccg agct
//
LOCUS       DASQQQ010000351         3250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_355793, whole genome shotgun sequence.
ACCESSION   DASQQQ010000351 DASQQQ010000000
VERSION     DASQQQ010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355793"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VE646_12975"
     CDS             <1..564
                     /locus_tag="VE646_12975"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZD80936.1"
                     /translation="AAWRVRRFREKPDAETVRELLGTGTHYWNSGLFCGRASVFLEEY
                     GRHLPLMRRAIDAAVEDWETDRSGALERYYEAVEATSIDYGIMQETDRAVILPAADWE
                     WDDLGSWESLSRWLPVSEDGNVAVGDCQLEGCADVIAFADGGRLAALGVSDVVIVRTG
                     DATLVAARDRLDEIRRFVRRLTTDGAR"
     gene            561..1112
                     /locus_tag="VE646_12980"
     CDS             561..1112
                     /locus_tag="VE646_12980"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZD80937.1"
                     /translation="MTARGLGIALALGVLVSACVGPYAERPPAEGESPRPAADIGRRD
                     EPEADRPSSWAEEGTLVGYVTPDGDFHTDSVPADSGRIDRRTVVTGTVRRASPVADPS
                     TAGYRVQVFAAQDRATAEAVAARLEGRLNGQPVYVERQDPWYKVRVGDFATREEADRL
                     RKRLVEMGYPEAWTTRATIRPSR"
     gene            1109..2332
                     /locus_tag="VE646_12985"
     CDS             1109..2332
                     /locus_tag="VE646_12985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HZD80938.1"
                     /translation="MSPPLVLIDPPQGPFWPLAATRPVAALLGGARTFHARWSQGQGQ
                     VAALLCDPEVSGCAFRSGAPAMINAWPDPTLGYRVAVSTWLPPREWIFGDAPATHVCQ
                     GTPVAWRLDTAAARRLARQPAVADSARETLRSLDLPATEGGGRSIDSIWELVEHNAEL
                     LIEDAADFTGAALEPEARVAVIGPAPRAAAGVEVGPFAVLDSTVGPILLDHGARVTSH
                     ASLRGPLYVGPGSAILGGEVGGGTSIGPDCKVRGEVERTIFQGYANKAHDGFVGHSVL
                     GEWVNLGAGTTTSDLKNTYGPVRVEGPAGRVDTGLLKVGSFLGDHVKTGIGSLLTTGA
                     RLGVATHFFGGRGMSPTWLPNFSWFDGADRQAVRFEDFRRAAGKAMARRGQQLESGEE
                     AILLALAACSARRGG"
     gene            2429..>3250
                     /locus_tag="VE646_12990"
     CDS             2429..>3250
                     /locus_tag="VE646_12990"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZD80939.1"
                     /translation="MRVLITGIEGFVGRHLAAALASVGYEVAGTALSPAAAEGPWRVI
                     ELDVGDPDRVARVIDEEHADAIVHLAGRASVADSFREPVVTYEVNLGGALHLLEACRR
                     TGVGRLLLVTSCEIYGELDPMDGPVAEQRTMAPVSPYGGSKAAQDLLGEQYARAFGLP
                     VVRARSFPHTGPGQQESYLFPSVARRVAEAEAGLGPPTIKVGNVDVVRDLLDVRDVAR
                     AYVLLLERGRPGEAYNVCSGRGRTLRDSLAGLCRLARLPVHLESDPSRERPADIPW"
BASE COUNT          433 a         1067 c         1246 g          504 t
ORIGIN      
        1 gcggcgtggc gggtccgacg gttccgcgag aagcccgacg ccgagaccgt ccgggagctc
       61 cttgggacgg gcacccacta ctggaacagc ggtctcttct gcggacgggc gtccgtcttc
      121 ctggaagagt acggccgcca cctgccgctc atgcgccggg cgatcgacgc cgccgtcgag
      181 gactgggaga cggatcggtc gggcgccctc gagcgttatt acgaggcggt agaggccacc
      241 tccatcgact acgggatcat gcaggagacg gatcgggcgg tgatcctgcc cgccgccgat
      301 tgggaatggg acgacctggg atcgtgggag agcctgtcgc gctggctgcc ggtctccgag
      361 gacggcaacg tcgcggtggg ggattgccag ctggaggggt gcgccgacgt gatcgccttc
      421 gccgacggtg gccggctggc cgcgctgggg gtctcggacg tcgtcatcgt ccggacaggg
      481 gacgccacgc tggtggcggc gcgggaccgg ctggacgaga tccgtcggtt cgtccgccgg
      541 ttgaccacgg atggcgcccg atgactgctc gggggttggg catcgcgctc gccctagggg
      601 tgctcgtctc ggcctgcgtg gggccgtacg ccgagcgccc tcccgcggag ggcgagagcc
      661 cgcggccggc ggccgacatc gggcgtcgtg acgagccgga ggccgatcgc ccatcctcct
      721 gggctgagga agggaccctg gttgggtacg tgacccccga tggcgacttc cacacggaca
      781 gcgtgccggc tgattcgggg cggatcgatc gccggaccgt ggtcaccggg accgtgcgca
      841 gggcctcgcc ggtggcggac ccctcgacgg cgggctatcg ggttcaggtc tttgccgcgc
      901 aggatcgcgc caccgccgag gcggtggccg cgcggctgga aggtcggctg aacggccagc
      961 cggtctacgt ggaacgccag gatccctggt acaaggtccg cgtcggggac ttcgcgaccc
     1021 gcgaggaggc cgatcggctg cgaaagcggc tggtcgaaat gggttacccg gaggcgtgga
     1081 ccacccgggc gacgatccgc ccatcgcggt gagtcccccg ctggtcttga ttgatccgcc
     1141 ccaggggccc ttctggccgc tcgcggccac ccggccggtg gcggcccttc tgggcggagc
     1201 gcgcaccttt cacgcgcgct ggagccaggg tcagggacag gtcgcggcgc tgctgtgcga
     1261 ccccgaggtg agtggctgcg ccttccgctc cggtgcaccg gcgatgatca acgcctggcc
     1321 ggacccgacc ctgggatacc gggtggccgt ctccacctgg ttgccaccac gcgagtggat
     1381 ttttggcgac gcgccggcga cccatgtctg ccaggggact ccggtggcct ggcggctgga
     1441 caccgcggcg gcgcggcgcc tggcccgcca gccggcggtg gcggactcgg cacgcgagac
     1501 cctgaggagc ctcgatctcc ccgccacgga aggcggggga cggagcatcg actcgatctg
     1561 ggagctagtg gagcacaacg cggagctcct gatcgaagac gcggccgatt tcaccggcgc
     1621 ggcgctcgag cccgaagccc gggtggccgt gatcggcccc gctccccggg ctgcggccgg
     1681 cgtggaggtg ggtccgttcg ccgtcctcga ctccacggtc gggcccatcc tgctcgacca
     1741 cggcgcccgc gtgacgtctc atgccagcct gcggggaccg ctctacgtcg gtcccggcag
     1801 cgcgatcctc gggggcgagg tgggtggggg gacctcgatc ggccccgact gcaaggtccg
     1861 gggcgaagtc gagcggacga tcttccaggg ctatgccaac aaggcccatg acgggttcgt
     1921 cgggcactcg gtgctggggg aatgggtcaa cctcggggcg gggacgacga ccagcgatct
     1981 caagaacact tacggaccgg tgcgggtgga gggccccgca ggccgggtcg acaccggtct
     2041 gctcaaggtg gggtcgtttc tgggcgacca tgtgaagacg gggatcggct ccctcctcac
     2101 gacgggtgcg cggctcgggg tggcgaccca cttcttcggc gggcgtggca tgtcgccgac
     2161 ctggctgccc aacttctcgt ggttcgacgg ggcggaccgg caagcggtcc gcttcgagga
     2221 cttccggcgt gccgcgggaa aagccatggc acggcgcggg cagcagctgg aatccggcga
     2281 ggaggccatt ctgctggctc tagcggcttg ctcggctcgg cgcgggggat aacttcaccg
     2341 gcgttggatc gtggttctcc ctggggcggg ctgccttcga tcgcccgccg ctggccggcc
     2401 ccgtggcgct tctcccaccc attgccctat gcgcgtcctc atcaccggca tcgagggatt
     2461 cgtcggacgg catctggcgg cggcgctcgc atcagtggga tacgaggtgg cgggaaccgc
     2521 gctctcgccg gcagcggcgg agggcccgtg gagggtcatc gagctggatg tgggggaccc
     2581 cgatcgcgtc gcccgggtga tcgacgagga gcacgcggac gcgatcgtgc atctggcggg
     2641 acgggcgagc gtcgcggatt cgttccgtga gccggtggtc acctacgaag tgaacttagg
     2701 cggcgccttg catctgctgg aggcctgccg gcggacgggc gtcgggcggc tactgctcgt
     2761 gacctcgtgc gagatctacg gcgaactcga ccccatggac ggtccggtgg cggagcagcg
     2821 aaccatggcc cccgtcagcc cgtacggcgg cagcaaggcg gcccaagatc tgctcggtga
     2881 gcagtacgcg cgagcgttcg ggctgccggt cgtgcgggcg cgctcctttc cacacacggg
     2941 gcccggccag caggagtcgt acctcttccc gtccgtggct cgtcgtgtcg ccgaagcgga
     3001 ggccggtctc gggccgccga cgatcaaggt cgggaacgtg gatgtcgtgc gcgacctgct
     3061 cgacgttcgc gatgtcgccc gggcctatgt cctgctgttg gagcgtggac ggccgggcga
     3121 ggcctacaat gtgtgcagcg ggagaggccg gacgctgcgg gattcgctgg ccgggctgtg
     3181 ccgactggct cgcctgccgg tccatctgga aagcgatccc tcgcgggaac ggccggccga
     3241 catcccatgg
//
LOCUS       DASQQQ010000352         4637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_355836, whole genome shotgun sequence.
ACCESSION   DASQQQ010000352 DASQQQ010000000
VERSION     DASQQQ010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355836"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VE646_12995"
     CDS             <1..589
                     /locus_tag="VE646_12995"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HZD80940.1"
                     /translation="RGWAGKPTHPPLTDFPIVGYVLAAVFDVLSFASHSGSGGSVSRF
                     ARDPFTAGTYVMIAGAVVSLGAATTGFWDWWKGIDRAPTGPLGRAPHTQVWRTINTHM
                     AIMLTVTAIAVADIVLRLVQTDAHHATVGVMILSVAAALLVAVGATYGGSLVFDHQFN
                     VEDLSASTAWDQSEADQTPQDKRAAREMAPEEQPS"
     gene            628..1281
                     /gene="folE"
                     /locus_tag="VE646_13000"
     CDS             628..1281
                     /gene="folE"
                     /locus_tag="VE646_13000"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HZD80941.1"
                     /translation="MPPPADADRDEGWAALHPRRISLEQWDRFQGYVAEILAALGMDL
                     NTPGTAQTPERLLRALVDSTEGYEGDPNVLTAFPTECRGGADCRISQVIEGPISFFAL
                     CEHHALPFQGAAYVGYVAHERIIGLSKITRLVRLFARRFTVQERIGQQVADALVQLMQ
                     PHGVAVYLEAAHLCTQMRGVREEGSTTQTSFWRGTYEQDPDLRRDFLQTISTRGVGR"
     gene            1278..2084
                     /locus_tag="VE646_13005"
     CDS             1278..2084
                     /locus_tag="VE646_13005"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZD80942.1"
                     /translation="MMPLTALWERATPAGPALSSDLERLYGGRLDLPAAGVVANFVSS
                     LDGVVALPGVAHSSQMISDRSEADRFVMGSLRAHADAVMIGAGTLRGAPDSLWSPAFI
                     YPEAAPWFVALRHRLGRSQDPRLVILTAKGDIDTTHPALQEGALVLTTARGAAALRDR
                     LPSSSSALAVGDGTSVDLRIAIGVLRRMGYRLILSEGGPTVVGELLRARLLQELFLTV
                     SPMIAGRSAMGGRPGLVDGAEVLPSDRLGGQLMSIRTHGSHLFLRYAFRG"
     gene            2158..3915
                     /locus_tag="VE646_13010"
     CDS             2158..3915
                     /locus_tag="VE646_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HZD80943.1"
                     /translation="MREYAAIGDGRTVALVGSDGAIDWLPVPMVDGSTVFASLLDPER
                     GGSFELSPDVPALVQRRYVPGTGVLETTFRTPGGSVRVTDALTVPAEGLTPSRELVRK
                     VEGLGGEVPMRWRVRPAFGYGDLRTRIGRRGTVPIASSRGAALAVAAFGAGEPRLSSE
                     AIWGDFQIRGGATALLSLSLSDQEPLILPARDEIERRLAHTVRTWRRWSANLRAAGPW
                     RQAVERSAITLKLLVSSASGAIAAAPTTSLPEAIGGARNWDYRYCWIRDSAFTLDALL
                     RVGCRPEATAFFWWLMHASQLSQPRLQVLYRMDGGPGAKERGLRLRGYRGSRPVRVGN
                     GAVSQFQLDIYGDLLETAAIYAQSGSVIDPDIGARLSGMADLVSSIWRVPDAGIWEVR
                     SPPRHFTHSKMMAWIALDRAIGLAERGSIRSRRVERWRTARDQVEDFIWSNCWSEGKD
                     ALIRAPGDEDLDAALLLGVRFGFGSGTDRLRRTVDAIDRELRQGPLVRRYSGPDGLQE
                     PEGAFLPCSFWLVEALAGLGRRDEAERLMGDLVRLANDVGLYPEEIDERDGGFLGNFP
                     QALTHLALIEAALALGERR"
     gene            3912..4394
                     /locus_tag="VE646_13015"
     CDS             3912..4394
                     /locus_tag="VE646_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80944.1"
                     /translation="MSLWHAALGGLLGTFVLTTMVRGASEARLTRMDLPFLLGTAVTT
                     DRARAKAIGYVLHFLAGLVFAMGYGIGFLVLGRAGWLLGAAFGVIHGLFAGSVLVNAL
                     LPLVHPNMGTPMSGIGSAALLEPPGFMMLNYGRATPIVSLMAHTAYGVIIGLSVSLRP
                     "
     gene            complement(4443..>4637)
                     /locus_tag="VE646_13020"
     CDS             complement(4443..>4637)
                     /locus_tag="VE646_13020"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80945.1"
                     /translation="FDGFPLYTYAGDTGPGQATGQGIQGIWFAAPASGPKQGGSSGGS
                     SGYGGSSGGSGGYTRGGNGY"
BASE COUNT          626 a         1609 c         1666 g          736 t
ORIGIN      
        1 acgggggtgg gcgggcaaac cgacgcaccc cccgctcacc gacttcccga tcgtcggcta
       61 cgtgctcgcg gcggtgttcg acgtcctgtc gttcgcgagc cactccggct cgggcgggag
      121 cgtcagccgg ttcgcccgtg atccgttcac cgcggggacc tacgtgatga tcgccggggc
      181 cgtcgtctcc ctgggcgccg ccacgaccgg gttctgggac tggtggaagg ggatcgatcg
      241 ggcgccgacg ggccccctgg gacgagcacc gcacacccag gtctggcgca ccatcaacac
      301 ccatatggcg atcatgctga cggtgacggc gatcgccgta gccgacatcg tgctgcggct
      361 ggtgcagacc gatgcccatc acgccaccgt cggggtgatg atcctctcgg tggcggccgc
      421 gctgctggta gcggtgggcg cgacctacgg gggctccctg gtcttcgatc accagttcaa
      481 cgtggaggac ctgtcggcga gcacggcctg ggaccagagc gaggcggacc agacccccca
      541 ggacaagcgg gcagccaggg agatggcccc ggaggagcag ccgtcatgag gagctcttcg
      601 atcgggcctg tggaggggat cggacgagtg cctccgccgg cggacgccga ccgggacgaa
      661 ggctgggcgg ccctgcaccc gcgacggatc tcgttggagc agtgggaccg cttccagggc
      721 tacgtcgccg agatcctcgc cgcgctcggg atggacctga acaccccggg cacggctcag
      781 accccagagc gtctcctgcg ggccttggtc gactcgaccg agggctacga gggcgacccc
      841 aacgtgctga cggcgttccc caccgagtgc cggggtggcg cggactgccg catcagccag
      901 gtcatcgagg ggccgatctc gttcttcgcc ctgtgcgagc accatgcgct gcccttccag
      961 ggggccgcct acgtcggtta cgtggcgcat gagcgcatca tcggcctgtc caagatcacc
     1021 cgactggtgc gcttgttcgc ccgacgcttc accgttcagg agcgcatcgg tcagcaggtg
     1081 gccgacgccc tcgtgcagct catgcagccg cacggcgtgg cggtctacct ggaggcggcg
     1141 cacctctgca cgcagatgcg cggggtccgc gaggaaggtt ccaccaccca gacctcgttc
     1201 tggcgcggga cctacgaaca ggaccccgac ctgcgacgtg acttcctgca gaccatcagc
     1261 accaggggcg tgggacgatg atgccgctca cggccctgtg ggagcgagcc accccggcgg
     1321 gtcccgcgct ctcctcggac ctggaacggc tgtacggggg aaggctggac ctgcccgccg
     1381 cgggggtggt cgcgaacttc gtctcctccc tcgatggcgt ggtcgccctc cccggtgtgg
     1441 ctcactccag tcagatgatc agcgatcgga gcgaggccga ccgcttcgtc atgggatcgc
     1501 tccgggccca cgcggacgcg gtcatgatcg gcgccgggac cctcagggga gcaccggact
     1561 ccttgtggtc gccggccttc atctatcccg aggccgcccc gtggttcgtg gctctgcggc
     1621 accggctggg gcggagccag gacccccgtc tggtgatcct gacggccaag ggcgatatcg
     1681 acaccactca cccggcgctc caggagggag ccctggtgct gaccaccgcg aggggggcag
     1741 ccgccctgcg cgatcgcctg ccctcctcct ccagcgccct cgcggtggga gacggcacga
     1801 gcgtcgacct tcggatcgcg atcggcgttc tgcggcgcat gggataccgc ctgatcctgt
     1861 ccgaaggcgg tccgacggtg gtgggcgagc tcctgcgggc ccggctcctg caggagctgt
     1921 tcctcaccgt ctccccgatg atcgccgggc gatcggccat gggaggccga cccggactgg
     1981 tcgacggggc ggaggtgctg ccgtcggatc gcctgggcgg tcagctcatg agcatccgaa
     2041 cgcatggatc ccatctcttc ctccggtacg cgttcagggg ctgacgggcc cgctccggcc
     2101 ggtcccgccc ccggatcgct gggccgggtc ccccaccgcc gtgacggttt cctgcccatc
     2161 cgcgagtacg cggcgatcgg ggatgggcga accgttgccc tggtgggaag cgacggcgcg
     2221 atcgactggc tgccggtccc catggtcgac gggtcgaccg tgttcgcctc gttgctcgat
     2281 cccgagcggg gaggcagctt cgagctctcg cccgacgtcc ccgcgttggt gcagcgccgg
     2341 tacgtgcccg ggaccggtgt gttggagacc accttccgga cgcccggagg atcggttcgc
     2401 gtgaccgacg ccctgaccgt gcccgccgag ggactcactc cctcccgaga gctcgtccgg
     2461 aaggtggagg ggcttggggg tgaggtcccc atgcggtggc gggtccggcc ggcgttcgga
     2521 tacggagacc tgcggacgcg gatcggtagg cgaggcaccg tccccatcgc atcctctcgc
     2581 ggcgcagccc tggccgtggc cgctttcggg gccggagagc cgagactctc ctcagaggcc
     2641 atctggggcg acttccagat caggggtggc gcgaccgcgc tgctctcgct ctcgttgtcg
     2701 gaccaggagc ccctcatcct gccggcccgg gacgagatcg aacgtcgcct cgcccacacg
     2761 gtgaggacgt ggcggcgatg gagcgcgaac ctccgggccg ccggaccgtg gaggcaggcc
     2821 gtggaacgga gcgcgatcac gctgaagctg ctggtctcgt cggcctcggg cgcgatcgcg
     2881 gctgcgccca ccacgtccct gccggaggcc atcggagggg cgcggaactg ggactaccgc
     2941 tactgctgga tcagggattc ggcgttcacc ctcgacgccc tgctccgggt gggatgtcgg
     3001 ccggaagcca cggcgttctt ctggtggctg atgcacgcct cgcagctcag ccagccgcgc
     3061 ctgcaggtcc tctaccggat ggatggagga cccggggcga aggagcgggg tctaaggctg
     3121 cgggggtatc ggggctcccg cccggtccga gtgggcaacg gcgccgtgag ccagttccag
     3181 ctcgacatct acggcgacct actggaaacg gcggcgatct acgcgcagtc cggttccgta
     3241 atcgaccctg acatcggagc gcggctgtcc gggatggcgg acctcgtctc gtccatctgg
     3301 cgggtccccg acgcggggat ctgggaggtg cggagcccgc ctcgacactt cacccactcc
     3361 aagatgatgg cctggatcgc gctcgaccga gccatcgggc tcgcggagcg gggatcgatc
     3421 cgctcccgcc gggtcgagcg gtggcgcacg gctcgggatc aggtcgagga cttcatctgg
     3481 tccaactgct ggtccgaggg caaggacgcc ctgatccggg cgccgggcga cgaggacctg
     3541 gatgcggcgt tgcttctggg cgtccgcttc gggttcggct ccggcaccga ccgcctccgg
     3601 cggaccgtcg acgcgatcga tcgagagctt cggcaggggc ccctcgtccg ccgctactcg
     3661 ggccccgacg gactgcagga gcccgaaggg gcgttcctcc cgtgctcctt ctggctcgtg
     3721 gaggccctgg ccggcctggg ccgtagggac gaggccgagc ggctgatggg cgacctcgtc
     3781 cgcctggcca acgacgtagg tctgtacccc gaggagatcg atgagcggga cggggggttc
     3841 cttggcaact tcccgcaggc cctgacgcat ctcgccctga tcgaggcagc cctggccctg
     3901 ggagagcgga ggtgagtctg tggcacgcgg cgctcggggg gctcctggga accttcgtcc
     3961 tcaccaccat ggtccgcgga gcgagcgagg cacgcctgac ccggatggac ctgcccttcc
     4021 tgctggggac cgccgtgacc actgaccgtg cgcgggccaa ggccatcggc tacgtcctcc
     4081 atttcctcgc cgggctcgtg ttcgccatgg ggtatgggat cgggttcctg gtgctggggc
     4141 gcgcgggatg gctgctggga gcggcgttcg gggtgatcca cggcctgttc gccggcagcg
     4201 tgctggtcaa cgcgctcctg ccgctcgtcc accccaacat gggcaccccg atgtccggga
     4261 tcggatcggc agcgctgctc gagccccccg ggttcatgat gttgaactac ggtcgcgcca
     4321 ccccgatcgt gagcctgatg gcccacaccg cctacggcgt catcatcggg ctctcggtct
     4381 cgctcaggcc ctagtgcgag agcagacccg cgacgggctg ccggcccacc gggcggatcc
     4441 gctcagtagc cgttgcctcc gcgcgtgtag ccaccgctcc cgccgctgga gccgccgtag
     4501 ccgctgctcc cgccgctgga gccgccctgc ttggggccgg aggcgggggc cgcgaaccag
     4561 atgccctgga tcccttggcc ggtggcctgt ccggggccgg tgtccccggc gtaggtgtac
     4621 agggggaagc cgtcgaa
//
LOCUS       DASQQQ010000353         5092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_326657, whole genome shotgun sequence.
ACCESSION   DASQQQ010000353 DASQQQ010000000
VERSION     DASQQQ010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326657"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1835
                     /gene="infB"
                     /locus_tag="VE646_13025"
     CDS             <1..1835
                     /gene="infB"
                     /locus_tag="VE646_13025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZD80946.1"
                     /translation="PAPQPAPAPAAKAAPAPAAAAAPAKPELPTLKLTHGATAQDVAD
                     KTGRSAAEVVKLLFSMGEMVPATQSLSDDALSLLATELGYQAEIVGVEEEEVEPEEEV
                     DESRLQPRPPVVTVMGHVDHGKTLLLDAVRRSDVVSGEFGGITQHIGAYQVHVDGREI
                     TFIDTPGHEAFTAMRARGAGVTDIVVLVVAADDGIMPQTVEAIDHARAAGVPIIVAVN
                     KIDKPESDPIRVRQQLAAEGVQTVEWGGQFEFVDVSAKARTNIDTLLETILLVADIEE
                     LKADPEGRARGAVIDANLDKGRGPVASVLVQRGTLEVGDALVAGTTWAKIRAMLDENG
                     QTVKVAGPSKPVQILGWSAVPNAGDEFREAEDERQARHVAQEREAKVRAAEQVTAVRP
                     PTLQDLMAQAREAEVPELNLIVKADVQGSIGALLDAFAKIPQEEVRVNVIHSAPGAIT
                     ESDVSLALASSAIVVGFNVRPVANASAMAEREGVDVRTYRVIYDAINDIRSAASGMLK
                     PEEQQVELGRAEVRALFRVPRVGVVGGCYVLQGTIVRNARARVVRDGVVAYEGRIGSL
                     RRFKDDVREVAEGFECGIGIENFNDVHEGDVIEAYEVREVAREL"
     gene            1857..2150
                     /locus_tag="VE646_13030"
     CDS             1857..2150
                     /locus_tag="VE646_13030"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HZD80947.1"
                     /translation="MLVALERFDLRIPRCGSLKEKRHVVKTLSNALRQKFNVAVAEVD
                     HQDLWQRTAIAAAAVGNEAYHLRKVMHEVERFVDRWMEVELLQADLSLHSPED"
     gene            2150..2497
                     /gene="rbfA"
                     /locus_tag="VE646_13035"
     CDS             2150..2497
                     /gene="rbfA"
                     /locus_tag="VE646_13035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HZD80948.1"
                     /translation="MGGRPDRVSEEFREILAEEIPRLKDPRIGFVTVTHVRIRPDLRR
                     ARVFYTVMGDQAGRRATAAGLRSARAHLRQVVGSQVRMKVLPELEFEEDDTIETAERI
                     DELLGHLHEGEHE"
     gene            2490..3467
                     /locus_tag="VE646_13040"
     CDS             2490..3467
                     /locus_tag="VE646_13040"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HZD80949.1"
                     /translation="MSEEMARAAAALSGAREVALACHVNPDPDALGSMLGLSNHLRSR
                     GVSTVCSFGNDPFLPPRWLERLPGAEALVPPSTFPPAPGLMVTCDTASLDRLGVLVPS
                     ARAATELIWIDHHASNEGLGTIPLIDPTASSTAEMVVRLIRAMGDGMPDEAAVCLYAG
                     LITDTGRFQYQAVRPDTLRLAADLREHPFDHAALSQALYEDNRADYLRLLGALLQRLV
                     LEPELDLVWTYLTRSDLAEAGVEAADADDLIDVLRTAREADIAAVIKQQHDGRFKVSL
                     RSRGGHDVASVAARFGGGGHRLAAGYTSRHGLALTMDELRSALRDASGG"
     gene            3464..4318
                     /gene="truB"
                     /locus_tag="VE646_13045"
     CDS             3464..4318
                     /gene="truB"
                     /locus_tag="VE646_13045"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HZD80950.1"
                     /translation="MSAGGGPEGLLLVDKPSGMTSHDVVDVLRRRLGTRKVGHAGTLD
                     PMATGLLLVGAGRATRLLRFLGELPKTYQGTARLGIETDTLDAQGRVLRTAQVDVARP
                     ELEAAIAGLVGDSLQRPPAYSAVKVGGRKLYQAARRGQRLEAPPRPIHVARFELTRYE
                     PPDWDFLVRCSGGTYVRALASEVGSAVGPGAHLVRLVRTAIGPFLLRDAAPPERATPQ
                     PIERAVSHLPRLELGPEEARAAGHGSILGPAGMEGPYGVYGPGGELIGVWRDDGAKAR
                     PEVVLAGG"
     gene            4349..>5092
                     /gene="ribF"
                     /locus_tag="VE646_13050"
     CDS             4349..>5092
                     /gene="ribF"
                     /locus_tag="VE646_13050"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HZD80951.1"
                     /translation="MQIRRGVSSLPIEDGPTVVTIGFFDGVHLGHRAVLGRTVEAARE
                     RSARAVAVTFDRHPREVLTPGHEPRLLTTVDRKAELIAATGVDVLLILPFTKEFSRWP
                     AEEFVHRVLANGLHAAHVAVGGDFTFGHRAAGTLGTLHELGPGFGFTVQEVPPFLLDG
                     RRVSSSSIREALAEGDLAWPERALGRRYAVEGTVVGGAGRGHALGFPTANLETSPRLL
                     LPGTGIYAGRAGTGDGTYVAAVSVGTNPTF"
BASE COUNT          685 a         1770 c         1985 g          652 t
ORIGIN      
        1 cgccggcccc acagcccgcc cccgccccgg ccgcgaaggc cgccccggcg ccggctgccg
       61 ccgccgcccc cgccaagccg gagctcccca cgttgaagct cacgcacggg gccaccgcgc
      121 aggacgtggc cgacaagacc gggcggagcg ccgccgaggt ggtgaagctg ctcttctcga
      181 tgggcgagat ggtgccggcc acgcagtccc tgtccgacga cgccctgtcg ctcctggcca
      241 ccgagctcgg ctaccaggcc gagatcgtgg gggtcgagga ggaggaggtg gagcccgagg
      301 aggaggtcga cgagagccgc ctccagcccc ggcccccggt ggtgacggtg atgggccacg
      361 tcgaccacgg caagacgctc ctgctggatg cggtgcgccg cagcgacgtg gtctcgggcg
      421 agttcggcgg catcacccag cacatcggcg cctaccaggt ccacgtggac gggcgcgaga
      481 tcaccttcat cgacaccccg ggccacgagg cgttcaccgc catgcgcgcg cgcggcgcgg
      541 gcgtcaccga catcgtggtg ctggtggtgg cggccgacga cggcatcatg ccgcagacgg
      601 tcgaggcgat cgaccacgcg cgggcggccg gggtgccgat catcgtggcg gtgaacaaga
      661 tcgacaagcc cgaatccgac cccatccgcg tgcgccagca gctggcggcg gagggcgtgc
      721 agaccgtgga gtggggcggg cagttcgagt tcgtcgacgt ctcggccaag gcccgcacga
      781 acatcgacac gctgctggag acgatcctgc tggtggccga catcgaggag ctcaaggccg
      841 atcccgaggg ccgggcccga ggcgccgtga tcgatgcgaa cctggacaag gggcggggcc
      901 cggtggcctc ggtcctggtg cagcgcggga ccctggaggt gggcgacgcc ctggtggccg
      961 gcaccacctg ggcgaagatc cgggccatgc tggacgagaa cggccagacc gtgaaggtgg
     1021 ccgggcccag caagccggtg cagatcctcg ggtggtcggc cgtccccaac gccggcgacg
     1081 agttccgcga ggcggaggac gagcgccagg cccgccacgt ggcccaggag cgcgaggcca
     1141 aggtccgggc cgcggagcag gtcacggcgg tgcggccgcc cacgctgcag gacctcatgg
     1201 cccaggcccg tgaggccgag gtgcccgagc tcaacctgat cgtaaaggcg gacgtccagg
     1261 gctcgatcgg ggccctgctc gacgcgttcg ccaagatccc ccaggaggag gtccgggtca
     1321 acgtgatcca cagcgcgccg ggcgccatca ccgagagcga cgtctcgctg gcgctggcct
     1381 ccagcgcgat cgtggtgggc ttcaacgtcc ggccggtcgc caacgcctcg gccatggccg
     1441 agcgcgaggg cgtggacgtg cggacctacc gcgtgatcta cgacgcgatc aacgacatcc
     1501 gcagcgcggc ctcgggcatg ctgaagcccg aggagcagca ggtcgagctg ggccgggccg
     1561 aggtccgggc cctgttccgg gtcccccggg tgggggtggt gggcggctgc tacgtgctgc
     1621 agggcaccat cgttcgcaac gcgcgcgctc gggtggtgcg cgacggggtg gtggcctacg
     1681 agggacgcat cggctcgctc cggcgcttca aggacgacgt gcgcgaggtg gccgaaggct
     1741 tcgagtgcgg catcgggatc gagaacttca acgacgtgca cgagggcgac gtgatcgagg
     1801 cctacgaggt ccgcgaggtc gcccgagagc tgtagcccga tccgccggga ggagcgatgc
     1861 tcgtcgcgct ggaacggttc gacctgcgga tcccgcggtg cggctcgctg aaggagaagc
     1921 gccacgtggt gaagaccctg tcgaacgcgc tgcggcagaa gttcaacgtg gcggtggccg
     1981 aggtcgacca ccaggacctg tggcagcgca ccgcgatcgc cgccgccgcg gtgggcaacg
     2041 aggcctacca cctgcgcaag gtgatgcacg aggtggagcg gttcgtggac cggtggatgg
     2101 aggtcgagct gctgcaggcc gatctgtcgc tgcactcgcc ggaggactga tggggggtcg
     2161 gcccgaccgc gtctcggagg agttccgcga gatcctggcc gaggagatcc cccggctgaa
     2221 ggatccgcgg atcgggttcg tcacggtgac ccacgtgcgc atcaggccgg acctgcgccg
     2281 ggcccgcgtc ttctacacgg tgatggggga tcaggccggc cggcgggcca ccgccgccgg
     2341 gctgcgctcg gcccgcgcgc acctgcgcca ggtggtgggc agccaggtgc ggatgaaggt
     2401 gctgcccgag ctcgagttcg aggaggacga cacgatcgag accgccgaac ggatcgacga
     2461 gctgttgggg cacctgcacg agggcgagca tgagtgagga gatggcccgg gcggccgcgg
     2521 cgctctcggg ggcccgggag gtggcgctgg cctgtcacgt gaacccggac cccgatgccc
     2581 tgggctcgat gttgggcctg tcgaaccacc tgcgctcgcg gggggtgagc acggtctgct
     2641 ccttcggcaa cgatcccttc ctgccgcccc ggtggctgga gcgcctgccc ggcgccgagg
     2701 cgctggtacc gccctcgacc ttcccccccg ctcccgggct catggtgacc tgcgacacgg
     2761 cctcgctgga ccggctgggg gtcctggtgc cctcggcccg ggccgccacc gagctgatct
     2821 ggatcgatca ccacgccagc aacgagggcc tgggcacgat ccccctgatc gaccccacgg
     2881 cctcctccac cgccgagatg gtggtccggc tgatccgggc catgggggac gggatgcccg
     2941 acgaggcggc ggtgtgcctg tacgccgggc tgatcaccga caccgggcgc ttccagtacc
     3001 aggcggtgcg cccggacacg ctccggctgg cagcggacct gcgcgagcac cccttcgacc
     3061 acgccgccct gtcccaggcc ctgtacgagg acaaccgggc cgactacctg cgcctgctgg
     3121 gggccctcct gcagcggctg gtcctggagc ccgagctgga cctggtgtgg acctacctca
     3181 cccgctccga cctggccgag gccggggtgg aggcggccga cgccgacgac ctgatcgacg
     3241 tgctgcgcac cgcgcgcgag gccgacatcg cggcggtgat caagcagcag cacgacggcc
     3301 gcttcaaggt cagcctgcgc tcgcgagggg ggcacgacgt ggcctcggtg gccgcgcggt
     3361 tcggtggggg agggcaccgg ctggcggccg gctacacgtc ccgccacggg ctggcgctca
     3421 cgatggacga gctgcgctcg gccctgcgcg acgcgtccgg cggatgagcg ccgggggcgg
     3481 tcccgagggg ctgctgctgg tggacaagcc cagcggcatg acctcccacg acgtggtgga
     3541 cgtgctccgg cgccggctgg ggacccgcaa ggtgggccac gccgggaccc tggaccccat
     3601 ggccaccggg ctgctcctgg tgggggcggg gcgggccacc cgcctgctgc ggttcctggg
     3661 cgagctgccc aagacctacc agggcacggc ccggctgggc atcgagaccg acaccctgga
     3721 cgcgcagggc cgggtcctgc gcaccgccca ggtcgacgtc gcgcgtccgg agctggaggc
     3781 ggcgatcgcc gggctggtgg gggactcgct gcagcgcccg ccggcgtact ccgcggtgaa
     3841 ggtggggggg cgcaagctgt accaggcggc ccggcggggg cagaggctcg aggcgccgcc
     3901 ccggccgatc cacgtggcgc gcttcgagct cacccggtac gagccgccgg actgggactt
     3961 cctggtccgg tgctcggggg ggacctacgt gcgggcgctg gcgtcagagg tcgggtcggc
     4021 ggtgggcccg ggggcccacc tggtccggct ggtgcgcacc gcgatcgggc ccttcctgct
     4081 gcgggacgcc gccccgccgg agcgggcgac gccgcagccg atcgagcggg ccgtctcgca
     4141 cctgccgcgg ctggagctcg gtcccgagga ggcccgggcc gcgggccacg gcagcatcct
     4201 ggggccggcc gggatggagg ggccgtacgg ggtgtacggg ccggggggcg agctgatcgg
     4261 ggtgtggcgc gacgacggcg cgaaggcccg tcccgaggtc gtgctggccg ggggctgagc
     4321 gctcgggatg ttcgatacgc tgcgcggcat gcagatccgc cgaggggtgt cctcgctgcc
     4381 gatcgaggac ggccccaccg tggtcacgat cggcttcttc gatggcgtcc acctggggca
     4441 ccgggcggtg ctgggccgga ccgtggaggc ggcgcgggag cgctcggccc gggccgtggc
     4501 ggtcaccttc gaccgccacc cccgcgaggt cctgaccccc ggccacgagc cgcgcctgct
     4561 caccaccgtg gaccgcaagg ccgagctgat cgcggccacc ggcgtggacg tcctgctgat
     4621 cctgcccttc acgaaggagt tctcccggtg gccggccgag gagttcgtcc accgggtgct
     4681 ggcgaacggc ctgcacgccg cgcacgtggc ggtggggggc gacttcacgt tcgggcatcg
     4741 ggcggccggg acgctgggga ccctgcacga gctcggcccg ggcttcggct tcaccgtcca
     4801 ggaggtcccg ccgttcctgc tggacgggcg gcgcgtgtcc tcctcctcga tccgcgaggc
     4861 gctggccgag ggcgacctgg cctggcccga gcgggccctg gggcgccgtt acgcggtgga
     4921 gggcaccgtg gtcggcgggg ccgggcgggg ccacgccctg ggctttccca ccgcgaacct
     4981 ggagacctcg ccccggctgc tgctgcccgg gaccgggatc tacgcggggc gggccggcac
     5041 cggcgacggc acgtacgtgg cggcggtgag cgtgggcacc aatcccacct tc
//
LOCUS       DASQQQ010000354         4188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_100292, whole genome shotgun sequence.
ACCESSION   DASQQQ010000354 DASQQQ010000000
VERSION     DASQQQ010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100292"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VE646_13055"
     CDS             complement(<1..312)
                     /locus_tag="VE646_13055"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZD80952.1"
                     /translation="MIWKRDEAEAGGTQERPRREVHEGVRRMAEQGSEVTIVGQGAKL
                     EGTVVSAGSLRIDGQVKGQVNADGDVMLSAKSQVEADIRAQNVSVAGRFKGNIVVKGR
                     AE"
     gene            complement(309..800)
                     /locus_tag="VE646_13060"
     CDS             complement(309..800)
                     /locus_tag="VE646_13060"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HZD80953.1"
                     /translation="MDLSTKTLTLLVLISLGLSALTLLFSALGSGRRARESKGPIQMD
                     EVLRGVLEGQAARIQRLESAARALNSTDKQQQGLIEGAVRHVGLVRYDAFEDVGGRLS
                     FSCALLDDHGNGVVMTSINGRQDTRVYAKPLTQGQSSYNLSVEEEEAIRQALAGPRER
                     VNT"
     gene            871..1641
                     /gene="larB"
                     /locus_tag="VE646_13065"
     CDS             871..1641
                     /gene="larB"
                     /locus_tag="VE646_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HZD80954.1"
                     /translation="MDERTLSRILSGVASGAVPVESALRDLRDLPFQQVPDAMVDHHR
                     ELRTGQAEAVYAPGKTPRQVRDTAAALAARASGAVFATKATPEQFHAVLEAVPTATYS
                     PRSRLIVVKRAGPEIAPGRLAVISAGTSDLPVAEEAAQTGEALGAIVDRISDVGVAGL
                     HRILAARERIDAADVVVVAAGMEGALPSVVAGLTPRPVVAVPTSIGYGAAFEGLAALL
                     SMLSACAPGICVVNIDNGFGAALVAHRILRPAPGSGPA"
     gene            1638..2831
                     /gene="larC"
                     /locus_tag="VE646_13070"
     CDS             1638..2831
                     /gene="larC"
                     /locus_tag="VE646_13070"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019542809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HZD80955.1"
                     /translation="MRIAYFDCVAGISGDMALGALIDAGADPDELRGTLDSLALESFR
                     LEISEGERQGIRALDVRVETSNQAVIRTYASIRTLLERSELPPDAERIAQRIFRRLAE
                     AEARIHRKEIELVTFHEVGAVDSIVDIVGTALCLTMLGIERVYASSVPTGMGMMRTEH
                     GAMPIPAPAVLELLQGVPLYSMGIPFELTTPTGAAILAATAEGYGEIPTMRIGSVGYG
                     AGSHPLDVPNVLRVLVGDALEEGSRQGAQEVTRELLLQTNLDDVSPEVHGFLVERLLA
                     AGAQDAWSTPIVMKKGRPAVSVSVLCSPELSGSIRSILFEETGTLGVRTSAVTKHALD
                     RRVLKVDTPFGVVAVKIGTHGGRPVSIAPEYEDCARAARGAEVPLLEVYAEAERMARE
                     RLHLL"
     gene            2882..3946
                     /locus_tag="VE646_13075"
     CDS             2882..3946
                     /locus_tag="VE646_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018104245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD80956.1"
                     /translation="MRTLRRLRLSAGKTRPNVDAVASTAGAVAAAATAAAIYLGSRSL
                     LHFDAALIGYATATVFLAFGVVYRYVVWVQSPPARRYLIRGWRSFLSWRNFRRFPTMV
                     PRALVSNLALQTFIGKRGRLRWLAHQSLFWGVVGATLITFPLTFGWLHFEAAGSGSRY
                     VLEVWGLRTVTFDPISWVGWVMFHALDATAVLVLLGCGYFLWRRFRDREASTGQRLGY
                     DFFPLVALVAISVTGLLLTFDSLVLEGQDYAFLAIVHMSVVVLTLVFIPFGKFFHVIQ
                     RPASIGVDMVKRTDIEREGVLTCRRCGAPLEGVRFVKDLETTMEELGLRFGGWVETCP
                     RCKRIQRGESYLRQVKGGFQ"
     gene            3943..>4188
                     /locus_tag="VE646_13080"
     CDS             3943..>4188
                     /locus_tag="VE646_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014139914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase"
                     /protein_id="HZD80957.1"
                     /translation="MSARRDFPVVAPGISDFREAGGEPAEDWRAPEREVERMVPTHCC
                     FCGVQCGMFLKLDGEGRVFGVEARDHDINHLRLCPKGV"
BASE COUNT          564 a         1489 c         1448 g          687 t
ORIGIN      
        1 ctcggcccgg cccttgacca cgatgttgcc cttgaaccgt ccggccaccg agacgttctg
       61 ggcccggatg tccgcctcca cctggctctt cgccgacagc atgacgtcgc cgtcggcgtt
      121 cacctgcccc ttcacctggc cgtcgatgcg gagcgatccg gccgacacca ccgtgccctc
      181 cagcttcgcc ccctgcccga cgatcgtgac ctcgctgccc tgctccgcca tccgtcgcac
      241 cccctcgtgc acctcgcgcc tcggccgctc ctgggtcccc ccagcctcgg cctcgtcccg
      301 tttccagatc atgtgttcac cctctcccgg ggccccgcga gcgcctgcct gatcgcctct
      361 tcctcctcga ccgacaggtt gtacgaactc tgcccctggg tcaggggctt cgcgtacacg
      421 cgtgtgtcct gccgcccgtt gatcgaggtc atcaccactc cgttcccgtg gtcgtccagc
      481 agcgcgcacg agaacgacaa ccgcccaccg acgtcctcga aggcgtcgta ccgcaccagg
      541 cccacgtggc gcacggcgcc ctcgatcagc ccctgctgct gcttgtcggt gctgttcagg
      601 gctcgggccg ccgactccag ccgctggatc ctcgcagcct gaccctccag cacaccgcgc
      661 agcacctcgt ccatctggat ggggcccttg ctctcacggg cccgccgacc cgagcccaga
      721 gccgagaaca gcagcgtgag cgccgacagc cccaggctga tgagcaccag caacgtgagc
      781 gtcttcgtgg acaggtccat ccgagccgac tccagaacgg gccatctacc tgggcttctg
      841 caggaccgcg agtatacaat catcgccctc gtggacgaac gaaccctctc ccggatcctg
      901 agcggcgtgg cctccggcgc ggtccccgtg gagtcggccc tccgggacct gcgggacctg
      961 ccgttccagc aggtgcccga cgccatggtg gaccaccacc gggagctgcg gaccggtcag
     1021 gccgaggccg tctacgcacc cgggaagacg ccacgacagg tccgggacac ggcggccgct
     1081 ctcgcggccc gggccagcgg tgccgtgttc gccaccaagg ccacacccga gcagttccac
     1141 gccgtcctgg aggcggtccc caccgccacc tacagccccc ggtcgcggct gatcgtggtg
     1201 aagcgcgccg ggcccgagat cgcgccggga cggctggccg tgatctcagc cgggacctcc
     1261 gatctcccgg tggccgagga ggcggcccag acgggggagg ccctcggcgc gatcgtggat
     1321 cgcatctcgg acgtgggggt ggcggggctg caccggatcc tggcggcgcg cgagcgcatc
     1381 gatgccgcgg acgtggtggt ggtcgcggcg gggatggagg gcgccctgcc gtccgtggtg
     1441 gccgggctca cgccgcgtcc cgtggtggcg gtgccgacca gcatcggtta cggggcggcc
     1501 ttcgagggcc tggccgcgct gctgtcgatg ctcagcgcct gcgcgccggg catctgcgtg
     1561 gtcaacatcg acaacggctt cggcgccgcc ttggtggccc atcgcatcct gcggcccgct
     1621 cccggatcgg gcccagcgtg aggatcgcct acttcgactg cgtcgcgggc atctcggggg
     1681 acatggcgct cggggcgctg atcgacgcag gggcagatcc ggacgagctg cggggcaccc
     1741 tggactcgct ggccctcgaa tccttccggc tcgagatctc cgagggggag cgccagggga
     1801 tccgggccct cgacgtccgg gtcgagacct cgaaccaggc cgtgatccgc acctacgcga
     1861 gcatccggac cctgctggag cgctctgagc tgcccccgga tgcggagcgg atcgcgcaga
     1921 ggatcttccg ccggctggcc gaggccgaag cgcggatcca ccgcaaggag atcgagctgg
     1981 tgaccttcca cgaggtgggg gcggtggatt ccatcgtgga catcgtgggc acggcgctgt
     2041 gcctgacgat gctcggcatc gagcgcgtct acgcctcgag cgtgccgacc gggatgggga
     2101 tgatgcgcac cgagcacggc gccatgccga tccccgctcc ggccgtcctc gagctcctgc
     2161 agggcgtccc cctgtactcg atgggcatcc cgttcgagct caccacgccc accggtgcag
     2221 cgatcctggc cgccacggcc gagggctacg gtgagatccc caccatgagg atcggatcgg
     2281 tcggctacgg cgccggctca catccgctgg acgtccccaa cgtccttcgg gtgctcgtgg
     2341 gcgacgccct ggaagagggt tctcgccaag gggctcagga ggtcacccgg gagctgctgc
     2401 tccagaccaa cctggacgac gtcagcccgg aggtgcacgg cttcctggtg gagcgcctgc
     2461 tggcggccgg cgcccaggac gcctggagca cgccgatcgt gatgaagaag gggcgtcccg
     2521 ccgtcagcgt ctccgtgctc tgctcaccgg agctgtccgg atcgatacgc tcgatcctgt
     2581 tcgaggagac gggcacgctc ggcgtccgga cgtccgcggt gacgaagcac gcgctggatc
     2641 gtcgggtcct caaggtcgac acgccgttcg gggtggttgc cgtgaagatc ggcacccacg
     2701 gggggcgccc cgtgtccatc gctccggagt acgaggactg cgcccgggcc gcccggggag
     2761 cggaggtgcc cctgctcgag gtctacgcgg aggccgagcg tatggcccgc gagcgcctgc
     2821 accttctctg aagcacggcc tgccgccgga cggcgacggc tggataggct gtagggctgg
     2881 gatgaggacc cttcgacggc tgcggctctc ggcgggcaag acccgcccga acgtggatgc
     2941 ggtggcgagc acggccgggg ccgttgcggc cgcggcgacc gcggcagcga tctacctggg
     3001 gagccgatcg ctcctgcatt tcgatgccgc cctcatcggg tacgcgacgg ccacggtctt
     3061 cctggccttc ggcgtggtct accgctacgt cgtctgggtc cagagcccgc cggctcgccg
     3121 gtacctcatc cgcggatggc gaagcttcct gtcctggcgc aacttccgac ggttccccac
     3181 catggtgcct cgtgcgctcg tatcgaacct ggcgctgcag acgttcatcg gcaagcgagg
     3241 gcggctgcgc tggctggccc atcagtccct gttctggggc gtggtgggcg cgaccttgat
     3301 caccttcccc ctgaccttcg gatggctcca cttcgaggcc gccggatccg gctcgcggta
     3361 cgtcttggag gtctggggac ttcgaaccgt caccttcgac ccgatcagct gggtggggtg
     3421 ggtgatgttc cacgcgctcg atgcgaccgc ggtgctcgtg ctgctggggt gcggctactt
     3481 cctgtggcga cggttccgcg accgggaagc gtccacgggc cagcggctcg gctatgactt
     3541 cttccccctg gtggccctgg tcgcgatctc ggtgaccggt ctcctgctca ccttcgacag
     3601 cctggtcctg gagggccagg actacgcgtt cctggccatc gtgcacatgt ccgtggtcgt
     3661 cctcaccctg gtcttcatcc cgttcggcaa gttcttccac gtgatccaac ggcccgcgag
     3721 catcggagtc gacatggtca agcgcaccga catcgagcgt gaaggggtcc tcacctgccg
     3781 gcgctgcggc gcgcccctcg agggggtgag gttcgtgaag gatctcgaga ccaccatgga
     3841 ggagctcggg ctgcggttcg gcggatgggt cgagacgtgt ccgaggtgca agcggatcca
     3901 gcgcggcgag agctacctgc gtcaggtgaa ggggggcttc cagtgagcgc ccgccgcgac
     3961 ttcccggtcg tcgcgcctgg gatctcggat ttccgcgagg cgggcgggga gccggccgag
     4021 gactggcggg ctcccgagcg cgaggtcgag cggatggtgc ccacgcactg ctgcttctgc
     4081 ggcgtgcagt gcgggatgtt cctgaagctg gacggcgagg ggcgcgtgtt cggggtggag
     4141 gcgcgcgacc acgacatcaa ccacctccgc ctgtgcccca agggggtg
//
LOCUS       DASQQQ010000355         5510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_53373, whole genome shotgun sequence.
ACCESSION   DASQQQ010000355 DASQQQ010000000
VERSION     DASQQQ010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53373"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="VE646_13085"
     CDS             complement(<1..1053)
                     /locus_tag="VE646_13085"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZD80958.1"
                     /translation="MSDRQPPNLDPALIRGLLQRRVSRRDVMRYAGIGAGALGMSAFL
                     AACGVSGSSGGASSSPTVDVGAFWDKQSLHHVLNFANWPYYIDTAKGSHPSLDLFTKK
                     TGIKVNYKPAIQDNASFFATLRPSLQAGKDTGWDLMVLTNGAQLTRLIEFGWLIPLDH
                     DKLPNFAKYASPSVKDPNYDPGNRYTLTWQSGFTGIAVNTKYIKDHITSVQSLWNSAY
                     KGKVGMMSDNTELGSVGLLALGMDPATPDVADWKKAAAKLQQQRDAGLVRNYYDQSYI
                     NALQNGDVWITQAWSGDIFQSQQSGYPELKFIVPDEGVMLWHDNMMIPAHAQNPLDAL
                     TYMDFVYQPKIAAMIAD"
     gene            complement(1226..1714)
                     /locus_tag="VE646_13090"
     CDS             complement(1226..1714)
                     /locus_tag="VE646_13090"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZD80959.1"
                     /translation="MAGTTPAVDAIDLRILEMLQEDGRRSYTAIAGELGVSEANVRQR
                     TKRMIGRGVAQIVAVADPISLGFGIMAAMNIRTRGGNRPEIADQIGAFAEVSYLALCA
                     GSADMLAEVVCRDQEHLLQITEAIEKIPGVEVIETFMYLRVVKQSEQWLTSGGARLLD
                     RA"
     gene            complement(1821..3062)
                     /locus_tag="VE646_13095"
     CDS             complement(1821..3062)
                     /locus_tag="VE646_13095"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HZD80960.1"
                     /translation="MRPGRLRWAAVRLGPAFDLLANYEPGGFFFERGGMGVSGTGTAT
                     AIEGPHPVLSEAATAASDLLSHVRPAEDGPAAVAVGAFPFAGGAGRLALPSRTVVRSW
                     PGRTWRLDVWREGLAPADHHRERWVGRAVPHDAFSQIQLRPDPEPRDYADAVAAATER
                     IRGGALRKVVLARSLLVDAERRLEPKQLLWRLRAVDPDCYAFAVPTQDGATLVGATPE
                     LLVEKWGREVHADPLAGSSPRFGDPEDDRGSAERLVGSDKDREEHAVVVEAVAHALGP
                     LCRELHHPREPELLGTANVWHLSTPFTGRLLDEVGSVLELVAALHPTPAVCGTPREEA
                     RRTLAELEPLDRGLYAGPVGWVDGHGDGQWAIALRCAEIGDQTARLFAGAGIVARSVP
                     EIELDETERKFRALLDALRWG"
     gene            complement(3059..3751)
                     /locus_tag="VE646_13100"
     CDS             complement(3059..3751)
                     /locus_tag="VE646_13100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HZD80961.1"
                     /translation="MASVTDLPHGAEKVHRVRSMFDSIAGRYDLVNRVMTFGLDVGWR
                     RRAVASLQLPGGSLVLDLACGTGDLCRELRAQGYRAIGFDFSAGMLGAARTEAPLVQA
                     DVLRLPLPDASADGATSGFALRNVVDLAAFFSEIGRVLRPGGRIALLDASRPERAAAR
                     AAHALYFGRIVPLLGGVLSDRSAYRYLPRSLAYLPAPTELAAMLRQAGFVEIGRTALA
                     AGAAQLLTGTRT"
     gene            complement(3739..4509)
                     /locus_tag="VE646_13105"
     CDS             complement(3739..4509)
                     /locus_tag="VE646_13105"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZD80962.1"
                     /translation="MRYRVEQLAAASDVSVDTVRYYQARGLLPPPEREGRVAWYGPEH
                     VERIRDVRALQRKGLTLAAIKRVLDGELGRADADLAAAVAAARGEGGSDELLTLERFA
                     QRSGVPASLIQAVEREGIRLGRSIDGQDRYTASDIDMVRAALRLLDFGLPLGDLLSLA
                     HDTHEAMTGVAERAVELFDEHVRKPIRDTAGDEEGSAAQLVEAFRTLLPAVTELVGNH
                     FRRLLLAAAEAHIERVGGAAEVRAAREESRRMDRAWRQ"
     gene            complement(4561..5367)
                     /locus_tag="VE646_13110"
     CDS             complement(4561..5367)
                     /locus_tag="VE646_13110"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZD80963.1"
                     /translation="MTHPEELLAEYVDGSLAEPRRVEVEDHLAVCSRCREETDLARAA
                     SAALVALPEEPVPFGVMNPVAAELRDRRERDRVPWYARLRWAAGVAVAAALVGLLAVV
                     LPRLGGVGTNGGGGQAAMSPGSSGNALRAPSSSFGAVFQDQPHMNYGAAGLEALAREV
                     AAHALAGPTTIPSVTPAPDQAVGEGLGSGFASLQPPVAARAQACVSRAVRPPGTDRLV
                     RLIRARYEGTPAFLSVYLESGGPGEPATKVVIWVVARSDCRILSFASRRL"
BASE COUNT          797 a         1995 c         1937 g          781 t
ORIGIN      
        1 gtccgcgatc atggcggcga tcttcggctg gtacacgaag tccatgtacg tgagcgcgtc
       61 cagcgggttc tgggcgtggg cagggatcat catgttgtcg tgccagagca tcacgccctc
      121 gtccggcacg atgaacttca gctccggata gcccgactgc tgcgactgga agatgtcgcc
      181 cgaccaggct tgcgtgatcc acacgtcacc gttctgcagc gcgttgatgt agctctggtc
      241 gtagtagttg cgcaccaggc ccgcgtcgcg ctgctgctgg agcttcgcgg ctgccttctt
      301 ccagtcggcg acatcgggcg tggcggggtc catgccgagg gcgagcagcc ccacgctacc
      361 cagctcggtg ttgtcgctca tcatccccac cttgcccttg tacgcggagt tccagagcga
      421 ctggacgctg gtgatgtggt ccttgatgta cttggtgttc accgcgatgc ccgtgaaccc
      481 cgactgccag gtgagcgtgt agcggttccc gggatcgtag ttggggtcct tcacggacgg
      541 gctggcgtac ttggcgaagt tcggcagctt gtcgtggtcg agcgggatca gccacccgaa
      601 ctcgatcagc cgggtgagct gggcgccgtt cgtgagcacc atgaggtccc accccgtgtc
      661 cttgccggcc tgcagcgacg ggcggagcgt ggcgaagaac gatgcgttgt cctggatcgc
      721 gggcttgtag ttgaccttga tcccggtctt cttcgtgaag aggtcgaggc tcgggtggct
      781 ccccttcgcc gtatcgatgt agtagggcca gttggcgaag ttcaggacgt ggtgcaggct
      841 ctgcttgtcc cagaaggctc ccacgtcgac ggtcggggat gagctggccc cccccgagct
      901 gccggacacg ccgcacgccg ccaggaaggc cgacatcccg agcgcgcctg ctccgatccc
      961 cgcgtaccgc atcacgtccc gccggctcac acggcgctgg agcaaccctc ggatcagggc
     1021 ggggtcgagg ttcgggggct ggcggtcgct catcgacttc tcctcccgtg cgtcggtggg
     1081 tccctacgct tctcgtcgca ggaccatcgt cggacgacgc gactcgttcc gcaaggggcg
     1141 atccgagaac cgaggcgtat cgtcacaggg gagggcgtcg gtgatccgtc gtgggatgga
     1201 tcctggttcg aggggtgaag gggggtcagg ctcggtcgag caggcgggct ccgccggagg
     1261 tcaaccactg ctcggactgc ttgacgaccc gcaggtacat gaaggtctcg atgacctcca
     1321 caccggggat cttctcgatc gcctcggtga tctgcaggag gtgttcctgg tcacggcaga
     1381 ccacctcggc cagcatgtcc gccgatccgg cgcacagggc cagataggag acctcggcga
     1441 aggcgccgat ctggtcggcg atctcgggcc ggttgcctcc gcgggtgcgg atgttcatcg
     1501 cagccatgat cccgaagccc aggctgatcg gatcggccac ggcgacgatc tgggccacac
     1561 cacggccgat catgcgcttg gtccgctggc gcacgttcgc ctcggagacg ccgagctccc
     1621 ccgcgatcgc cgtgtaggaa cggcgcccgt cctcctgcag catctccagg atccgcaggt
     1681 cgatcgcatc gaccgcgggg gtggttcctg ccacggggat cactcctgcc ctacgagatc
     1741 agctccacct gccctctcct ggcacgagtt tcgcacgcgc gaggtggttc ggcaccgtcg
     1801 gccgatccga gggcggaggc tcagccccat cgcagcgcgt cgagcagggc ccggaacttg
     1861 cgctcggtct cgtccagctc gatctcgggc accgacctgg ccacgatccc ggcaccggcg
     1921 aagaggcggg cggtctgatc gccgatctcg gcgcatcgca gcgcgatggc ccactggccg
     1981 tcgccgtggc cgtcaaccca ccccactggt ccggcgtaga gcccacgatc caacggctcc
     2041 agctcggcga gggtccgccg ggcctcctcc ctcggcgtcc cacagacggc gggggtggga
     2101 tgcagggccg ccaccagctc cagcacgctt cccacctcgt ccagaagccg gccggtgaag
     2161 ggcgtggaga ggtgccacac gttcgcggtt ccgagcagct ccggctcgcg agggtggtgg
     2221 agctcgcggc agagcggacc cagggcatgc gcgacggcct ccaccaccac ggcgtgctcc
     2281 tcccgatcct tgtccgatcc gaccaaccgc tcggcggatc cacgatcgtc ctcaggatcc
     2341 ccgaaccttg gcgacgaacc cgcgagagga tcggcatgga cctcccgccc ccacttctcc
     2401 accagcagct cgggggtggc acccactagg gtggccccgt cctgggtcgg caccgcgaac
     2461 gcgtagcagt ctgggtccac ggcccggagc cgccacagga gctgcttggg ctccaaccgg
     2521 cgctcggcgt ccaccagcag ggaccgggcc aggacgacct tccggagcgc gcccccacgg
     2581 atccgttccg tggccgcggc cacggcgtcg gcgtagtcgc gcggctcggg atcgggtcgg
     2641 agctggatct ggctgaaggc gtcgtggggg acggcccggc cgacccatcg ctcgcgatgg
     2701 tggtcggccg gggccaggcc ctccctccat acgtccaggc gccaggtccg accgggccag
     2761 ctccgcacca ccgtccgcga gggcaacgca aggcgtccgg ccccgcccgc gaaggggaag
     2821 gcgcccaccg ccacggcggc agggccatcc tccgcgggcc ggacgtgcga cagcagatcg
     2881 gatgcggccg tggcggcctc ggacagcacg ggatggggac cctcgatcgc cgtggcggtg
     2941 cccgtccccg agacccccat cccaccccgc tcgaagaaga agccgcccgg ctcgtagttc
     3001 gccaacagat cgaaggcggg tcccaggcgg acggcggccc atctcagccg gcccggcctc
     3061 acgtccgggt ccccgtgagc agttgggccg cgcccgcggc gagcgcggtg cgtccgatct
     3121 cgacgaaccc cgcctgccgt agcatcgccg ccagctcggt cggtgcgggc aggtaggcga
     3181 gggaccgggg gaggtaccgg taggcgctcc gatccgatag gaccccgccg agcagcggga
     3241 cgatgcgacc gaagtagagc gcgtgggcgg ccctcgccgc ggcccgctcc ggccgggagg
     3301 catccagcag cgcgatcctt ccccccggtc ggagcacccg gccgatctcc gagaagaacg
     3361 ccgccaggtc gaccacgtta cggagcgcga agccggaggt ggcgccgtcg gccgaggcgt
     3421 cgggaagggg gaggcgcagc acgtcggcct ggaccagggg agcctcggtc cgggcggcac
     3481 ccagcatgcc ggcggagaag tcgaagccga tcgctcgata gccttgagcc cggagctccc
     3541 ggcacaggtc acccgtcccg caggccaggt ccagcacgag ggatcccccc gggagctgca
     3601 gggaggccac ggcccgacgc cgccacccca cgtcgagccc gaaggtcatc acccggttca
     3661 ccaggtcgta gcgtccggcg atcgagtcga acatgctgcg gacccgatgg accttctcgg
     3721 ccccgtgcgg caggtcggtc actgacgcca cgcccggtcc atccgccggg actcctcgcg
     3781 cgccgctcgc acctcggcgg caccgcccac gcgctcgatg tgcgcctcgg ccgcggcgag
     3841 caagagccgg cggaagtggt tgcccaccag ctcggtcacg gcgggcagca gggtccggaa
     3901 ggcctcgacg agctgggcgg ccgacccctc ctcgtccccc gccgtgtcgc ggatcggctt
     3961 gcgcacgtgc tcgtcgaaca gctcgacggc ccgttcggcc acaccggtca tcgcctcgtg
     4021 cgtgtcgtgg gcgagggaca gcaggtcgcc caggggcagg ccgaagtcga ggagccgcag
     4081 cgccgcccga accatgtcga tgtccgaggc cgtgtagcgg tcctgcccgt cgatcgaacg
     4141 ccccaggcgg atcccctcgc gctcgaccgc ctggatcagc gatgccggca ctccgctgcg
     4201 ctgagcgaat cgctccaggg tgagcagctc gtcggacccg ccctctccgc gtgcggcggc
     4261 gacagcggcg gccaggtcgg cgtcggcgcg acccagctcc ccatcgagga cccgcttgat
     4321 ggccgccagg gtcaggccct tgcgctggag ggctcgcacg tcccggatgc gctccacgtg
     4381 ctcggggccg taccaggcca cccggccctc ccgctcggga ggaggcagca gccctcgggc
     4441 ctggtagtac cggacggtgt ccaccgatac gtcgctcgcc gccgcgagct gctccacccg
     4501 atacctcacg accggacact ctacgagtgt tcgctcgaac gttccagcgg gagggcgtgg
     4561 ctacaggcgc cgcgacgcga acgacaggat gcggcagtcc gaccgggcca cgacccagat
     4621 cacgaccttg gtggccggct ctccgggccc gcccgactcc aggtagacgc tcaagaacgc
     4681 gggggtgccc tcgtaccgcg cccggatgag ccggaccaga cggtcggtgc cgggcggccg
     4741 gaccgcccgg ctcacgcagg cctgggcccg ggccgccacc gggggctgca ggctcgcgaa
     4801 ccccgaaccg aggccctccc cgaccgcctg gtccggtgcc ggcgtgacgg aggggatcgt
     4861 ggtcgggccg gcgagcgcgt gggccgcgac ctcccgggcc aacgcctcga gacctgccgc
     4921 cccgtagttc atgtggggtt ggtcctggaa gaccgcgccg aaggacgatg acggtgcccg
     4981 gagcgcgttc ccgctcgagc cgggggacat ggccgcctgg ccgccgcccc cgttcgtccc
     5041 gaccccgccc agacggggga ggaccacggc caagagcccc accagggccg ccgccacggc
     5101 cacgcccgcc gcccagcgca gccgggcgta ccaggggacg cggtcccgct cacggcggtc
     5161 gcgcagctcg gcggcgaccg ggttcatgac accgaagggg acgggctctt cggggagcgc
     5221 gacgagggcg gccgaggcgg cccgggccag gtcggtctcc tcgcggcacc gggagcagac
     5281 ggccaggtgg tcctcgacct cgacccgccg gggctcggcc aacgagccgt ccacgtactc
     5341 ggccaggagc tcttcgggat gcgtcatctc gggacgggtc atacggctct cgcttcgtcc
     5401 gacgatccgg ggggctcggg ccgttccccg gggccgacgt gcatggcccg ggccagggcc
     5461 acccgcccgc gatgcaggcg ggacttcacg gtccccaccg gcacccccag
//
LOCUS       DASQQQ010000356        24211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_379959, whole genome shotgun sequence.
ACCESSION   DASQQQ010000356 DASQQQ010000000
VERSION     DASQQQ010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 24211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24211
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379959"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VE646_13115"
     CDS             complement(<1..348)
                     /locus_tag="VE646_13115"
                     /inference="COORDINATES: protein motif:HMM:NF027503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HZD80964.1"
                     /translation="MVAEGEILWQPSAERRDRSNLARYLRFLEQTRGRSFRDYAELWS
                     WSVDDLEGFWASVAEFFSVRWHRPPDAVLAERRMPGAVWFPGAELNYAERALAHRDDR
                     VAVGFGNESGDSRS"
     gene            442..1587
                     /locus_tag="VE646_13120"
     CDS             442..1587
                     /locus_tag="VE646_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HZD80965.1"
                     /translation="MREGGGMADRSDLQRYRENLRDEVDSAELYLAMAEREAQAELAT
                     LYRKLADIELRHAGFWEERLREAGAAVPARRITWRARVLRWLARRFGPRLVLPTVASL
                     EEINQHMYDRQPETGGTSLPADERSHARLLRTLAGSPRRGLEGGTLAQVEGRHRSVGG
                     NALRAAVLGANDGLTSNLSLVMGVAGASSSSTPVVVAGLSGLFAGAFSMALGEWVSVQ
                     SSRELYERQMAVEAEEIATIPDEEREELSLIYQAKGMSAQAADEFAARLMQDQALALD
                     AMAREELGIDPEELGGSPWVAAFASFALFALGAVIPLVPFVLASGAAATVASLVASGV
                     GLFAIGVAITLLTGRGALLSGGRQLAFGLAAAGVTFAIGSAVGSALG"
     gene            1663..1733
                     /locus_tag="VE646_13125"
     tRNA            1663..1733
                     /locus_tag="VE646_13125"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1695..1697,aa:Gln,seq:ctg)
     gene            2085..2414
                     /locus_tag="VE646_13130"
     CDS             2085..2414
                     /locus_tag="VE646_13130"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZD80966.1"
                     /translation="MNKSGLIAEVAKRTGASKAETGRSIDATMDVIRETVAKGDRVSL
                     VGFGTFERKRRNRRIARNPRKPQTPIVVPARDLPWFSAGKEFRDMVTSRRRRTAAKST
                     ARAASRR"
     gene            complement(2401..2610)
                     /locus_tag="VE646_13135"
     CDS             complement(2401..2610)
                     /locus_tag="VE646_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HZD80967.1"
                     /translation="MAQGTVKEYDEEQRTGTVLTDDLAEVEVDATSIEGAGIRSLRLG
                     QRVKFDLVEEGGRRVARTLRLVTFD"
     gene            complement(2619..3260)
                     /gene="cofC"
                     /locus_tag="VE646_13140"
     CDS             complement(2619..3260)
                     /gene="cofC"
                     /locus_tag="VE646_13140"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HZD80968.1"
                     /translation="MRVIAIPVKSLSRTKSRLGSELSPLERGALTLAMLEDVLDATAA
                     VPGWETWVISPDEVVLEIAARREVRPVPEAKPPLSSAVRQAEALSLEEEATALAIIPG
                     DLPLITPDALAQGLRTLGPVVLVRSTGEGTNLLLRRPPRSIPARFGPDSFRRHLTLAE
                     LRGLPASVVERPELSFDVDLPSDILTVLASERRGRTLEVCLEMDLAERLKVHA"
     gene            3359..3940
                     /locus_tag="VE646_13145"
     CDS             3359..3940
                     /locus_tag="VE646_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HZD80969.1"
                     /translation="MTTLAIAASPARIRSSAVYRVLLALAGSWLVAGLAQVEVRLPFT
                     PVPVTGQTLGVLLVGAGLGPGLGALSVGLYLAQGAVGLPFFSGGAGGVSFLGAAAATG
                     GYLWGFLLAATVVGFLARRGWDRTPGRAVAAMLLGEAVIYAVGLPWLMGTVDVGLARA
                     ISLGFTPFVAGDLLKLLIAAALVPAAWRVVPRR"
     gene            3967..4641
                     /locus_tag="VE646_13150"
     CDS             3967..4641
                     /locus_tag="VE646_13150"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZD80970.1"
                     /translation="MEPWYRVARTALLPPIGLWFNWRLEGLEAIPPEGPLIVAGNHLS
                     YLDPLAHGLFVDRAGRRPRFLAKRELFQNLFVRTVLLGAGQIPVDRGSGARAPLMDAA
                     RALEAGEVVVLYPEGTSTTTDPQFSPGRGRNGAIRLSLMTGVPILPVATWGGQYVWRR
                     GERIRPTFGRPILMTAGAPYDPSARSRSAEDRPSVPQLTAGLMRELSGLVDELRTRYP
                     RRWTPG"
     gene            4652..5836
                     /locus_tag="VE646_13155"
     CDS             4652..5836
                     /locus_tag="VE646_13155"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZD80971.1"
                     /translation="MSQERRRGFATRAIHVAPPVVDQQTPSVPIYQTSTFRFDTSDAY
                     AETIAFRRPGYTYTRGYGNPTVQAFEETVADLEGTSTALSFASGMAAIHTTITTLAGR
                     GDRVVAGRELYGGTYSMFTTVLPRYGIGVDLVDPHDPSAVASALPGAAAFYVETIANP
                     TVTVADLQVLGELCAEAGVPSVVDNTFASPYLCTPARYGFDLVLHSATKYLGGHHDLT
                     GGVACGSVESIRRLRQMVIETGSSLGPFDAWLALRGLMTLELRMQRHSASAAALAAHL
                     QGHPRTEAVHYPGLASHPQHAVARRILPRGSGGMLAFEVTGGVEAGKRFCDALRVAWV
                     ATSLGGTHTLVGHAASTTHRQMDPEARRAAGIGDGLVRVSVGLEDLDDLLEDVDQALE
                     AA"
     gene            5833..6993
                     /locus_tag="VE646_13160"
     CDS             5833..6993
                     /locus_tag="VE646_13160"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HZD80972.1"
                     /translation="MRRRVAIVFGGRSAEHEISCISARSVMDALDPDRYEIVPIGVTK
                     DGRWQLVPGGPPALPADPATALPQVGDASGPEVSLDQEPGARALVAEDGSRTPIDVVF
                     PVMHGPYGEDGSIQGFLEMAGVPYVGAGVLGSAVGMDKAVQKVLFREAGIPVVEHEVV
                     REREWQEDREAVGARAGHLGYPMFVKPAALGSSVGIRKIHGVQELSSAVDEAFRYGRK
                     VVLERSVEGAREIEVAVLGNDDPVASVAGEIVPAGGHEFYDYEAKYVDEHGANLLVPA
                     ELPPGALDEVQRLAIAGFRAVECSGMARVDFFLLSDGSLLLNEINTIPGFTRISMYPK
                     LWEASGLAYPALIDRLVELALERHAGERKRGTVLGAGSGSSPQARAEGPNGL"
     gene            complement(6990..7223)
                     /locus_tag="VE646_13165"
     CDS             complement(6990..7223)
                     /locus_tag="VE646_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HZD80973.1"
                     /translation="MVHAYILIQTEVGKAAQVADEVRGIAGVTEAEDVTGPYDVIVRA
                     ESRNVDELGKLVVAKIQAIDGITRTLTCPVVHL"
     gene            7365..7559
                     /locus_tag="VE646_13170"
     CDS             7365..7559
                     /locus_tag="VE646_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80974.1"
                     /translation="MGRGKDPKTSSGFLERYRRVRKPMPPPERVILDQRRRRREEQAR
                     REIEEEVDRGSETDDVRRSP"
     gene            7537..8178
                     /locus_tag="VE646_13175"
     CDS             7537..8178
                     /locus_tag="VE646_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HZD80975.1"
                     /translation="MMFADRREAGDRLAQALERFRGSDAVVLAIPRGGVIVGAVVSEA
                     LDLPLDVVVPRKVGAPGNPELGLGAVAPGVRVIDPRMVEALGVGQAYLEREITLQERE
                     IERRLAAYRAGRPAVAVRDRTAIVVDDGVATGGTAIAALRWARAQGAHEVVLAVPVAP
                     GASIRRLDREADRVLILAAPEPFYAVGEWYGRFDQTSDEQVVEALARAAESRT"
     gene            8175..8495
                     /locus_tag="VE646_13180"
     CDS             8175..8495
                     /locus_tag="VE646_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80976.1"
                     /translation="MSAVELGIATVLALGGIRSLWRWTRRRFEGTDLVDHLLYALFVT
                     GRVGLWFSLAGLFLIYAATTIDGRSTSALDRFRWYLIVPIAMAAAQFAAGYFLGNRAP
                     RDET"
     gene            8591..9628
                     /locus_tag="VE646_13185"
     CDS             8591..9628
                     /locus_tag="VE646_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80977.1"
                     /translation="MARVIRSRSALVLGALVLVLALVAGLGAVQAEPRPDLPPVGPKR
                     LIASTLEAIAQRGPVSGEVHTHVDLGLPQLPGNLAGPSGPADVLLSDQTFRVWRSADG
                     VRVAQVLPFGERDAVGNATDAWFWDSQRFTAWHASVPAQAGSSPQIPSLGDLTDLVGT
                     TLRRLQPYAEVRVTDPVMVAGRSAYVLRLVPSSGDTLIGHVDVAVDARTRVPLRISVV
                     AAGGSAPVIEAGFTSVSFGPIDPQMFSFSPPQGATVKQVRPHTGMEPAAPEHGASSGD
                     LPAGVRVFGRGFGTVLAIRVDTVPQDLGPLFPYAGPLGSADVVERGDHAWIVAGAVPP
                     GRLAEVEPTLP"
     gene            9625..10545
                     /locus_tag="VE646_13190"
     CDS             9625..10545
                     /locus_tag="VE646_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018384485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD80978.1"
                     /translation="MTALVARGLTKRYGGVDVVDGLDLRVERGELYGFLGPNGAGKTT
                     TIRMSLGLIFPSAGEVELLGRPVFGDGRSQALARTGAMIEEPGFYRFLSGRRNLEAFA
                     RAGGGDVRDRLSRIDEVLELTGLSGAAGKRVKAYSQGMRQRLGIALALLGEPEMLVLD
                     EPTNGLDPSGMREIRRLLRRLADHGTTVFVSSHLLSEVEAMCDRVGVLSRGRLVAEGP
                     PGKLRGTAERLRIDVDDRDRALAVLAGVPGVEASADGALVRVRLRGAEPAAVNAALVS
                     AGLAVSALVPEQDTLEEVFLALVEEGHVPG"
     gene            10532..11338
                     /locus_tag="VE646_13195"
     CDS             10532..11338
                     /locus_tag="VE646_13195"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZD80979.1"
                     /translation="MYRVELAKAARRWRTWLVAAALGGIPALIVIAVKLSPPANGGGQ
                     DAPPFLMQIVRNGLFAPLTGLAVIQPFFLPLAAGLFAGDAIAGEAQGGTLRYLLVRPV
                     GRARLVLAKYAGAMTLLGLMLLWVVAVGIAFGAVAFGLGPLPTLSGSTLGTGSAVLRI
                     VGAATYMFLVVSGIVAVGTFISTLTDSGPGATVATVILGIASEIVDNIPNLKVVHPYL
                     PTHGWLAFADLFRFPIAWGAMWDGVIVSLVYTVVFLGAALLWFRRKDVAS"
     gene            complement(11378..12895)
                     /locus_tag="VE646_13200"
     CDS             complement(11378..12895)
                     /locus_tag="VE646_13200"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HZD80980.1"
                     /translation="MHVKARVLHDNPSPEELRKLTEEMPQCRLTEFGNVNVQTRATSR
                     SVGSTYIVTDDPSTAGGQSMDREEYARIAELQDAYIAQQEMLVIDGYIGNDPDFRTPA
                     RLSIERANANVAGMQQKLYFPREGGEPEVQVIYTPNLPVPGYPDDRVIAVDLENGVTR
                     VLNSDYFGESKKGGLRMWNRIVYDRGGLSLHAGCKVVPTGAGDKVFLIIGLSGTGKTT
                     TTFTTQNSSLPVQDDFVALMPGGKTYGTENGCFAKTFSLDPDFEPNIYNAVISPDTYL
                     ENVYQDQQGNVNFFETSYTKNGRAVFGMDALGRHKDPRAVGPVDFLLILNWNENIIPA
                     VSKLNQEQAAAYFMLGETTGTSAGGKAEEGKFLRVPGTNPFFPMPDGLQGNRILELLG
                     SHPIEVFLLNTGRVGGKPDDERSKKVKIPHSSAVVKAIAEGTITWDQDDDSGTQVASA
                     VPGIEDLELLQPIRLYERQGRMREYQGFVERFRAERRAHLEGFRELSEQIVRAVG"
     gene            13027..14046
                     /gene="thiL"
                     /locus_tag="VE646_13205"
     CDS             13027..14046
                     /gene="thiL"
                     /locus_tag="VE646_13205"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HZD80981.1"
                     /translation="MTKRMELSEDELIAAIRRVLSGPGPGVVLGMGDDAAVVDRGSGS
                     LVLTTDMLVEAVHFDRALVPARDLGAKAIVVNVSDIAAMAASPRYALVSLAVPEDVGA
                     KWVVELAGGMRDACEEYALTLVGGDTNRADEVVISVTVVGEVAPGHHITRGGAHPGDL
                     IVVTGTLGAAAAGYLLSAMPPSRAARGLSGPWGRDALDALLHPVARVGEGQTLAQAGA
                     TAMMDLSDGLAKDLSRLCAESGVGARVEAARVPVSRVVREGAAFLGADPLELAIGGGE
                     DYELLATMDATDLDRARAELDERFGVSLTEVGVISESGLTLVGADGIEGPLAPKGWDH
                     FRRAT"
     gene            14036..14845
                     /gene="thiD"
                     /locus_tag="VE646_13210"
     CDS             14036..14845
                     /gene="thiD"
                     /locus_tag="VE646_13210"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HZD80982.1"
                     /translation="MRPDARVPRALTIAGSDSGGGAGIQADLNTFGALGVYGTTALTA
                     VTVQNTRGVAGYQELSPRTVGDQIRAVAEDIGLDAAKTGMLASRPIVAAVADAVAQAE
                     VPNLVVDPVFVSKHGHSLLAPDAVEALVSRILPLATLVTPNLPEAAGLAGFGVTTAED
                     MRRAAEAILRLGPSAVLVKGGHRTDGRADDLFLDGERLVWIEGQRIDVRNTHGTGCVL
                     SAAIAAELARGRDLLTAVRTGKAFVTEAIRHALPLGHGIGPVDPLWRSRQG"
     gene            complement(14864..17263)
                     /locus_tag="VE646_13215"
     CDS             complement(14864..17263)
                     /locus_tag="VE646_13215"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HZD80983.1"
                     /translation="MSDGRLTVVTGGDVYLSVGGVRPFLEGSGYEVQEHAATFEELLA
                     AVRKHRPDAVVMGGDIANAGRIDALRAASPDTRIIVVASAGNAPEGADALVPRGSSLS
                     AIASALAAQLVEPAVPSPTGTGASAAPRGPGRLRVLQFVAAAAVALSVGGVLLVMSGG
                     TAPNQPIRAGRATPGTSGPVAPTPGSSSPLQEEVQSSLIQLVSAIEDHRFEATPALAQ
                     ELVRNLQQASDRGVPVDPLNAQVTSSLRPVIAQVPTEVLPSLRSILDGLMPEVGPRIE
                     FQGDGGFGEVPVGSSSDPQTVAVLNVGDRALQISEVGIAGEAPGAFSVPSGDCEGRTL
                     TPEGSCAIQVIFSPTRTGPQTAELTVSGPEGVAPGAFALEGTGAFIQLLDTDAPRIRC
                     DDPPLGWQGADVLIACTASDAGSGLASAADASFQLSTHVAPGSEDAEASTDARRVCDR
                     EGNCAQAGPFGGIHVDQRAPEVTCRQPTHLWQGANVQVHCVSRDGGSGLAAAERSFVL
                     RTSIQRGRETAAARTGARRVCDAVGNCTTVGPFENLRVDRKAPGASCERPPHAWQATN
                     GSVRCSGTDGGSGVRAGDRSFRLTTAVPAGRAEANARTGTRRVCDAVGNCATAGPVAG
                     FRIDRAAPRIVLSGIADGAVLLLHQSAASDASVSCVDAGSGVASCSAPGSIDTSEVGR
                     HSFTATAIDRVGNVGTVSVRYVVTYGVELRYRPSEPSRKIIIRLVDAAGKNVSSSSVS
                     VTAVDIDGSPLHKPFVYSRSKRAYFLTVPGKKDSARHVLHFVARDDPVVHSAPFILGE
                     D"
     gene            complement(17412..17603)
                     /gene="rpmB"
                     /locus_tag="VE646_13220"
     CDS             complement(17412..17603)
                     /gene="rpmB"
                     /locus_tag="VE646_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003785965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HZD80984.1"
                     /translation="MAAVCDICGKKPFFGKQVSHSHRRSNRRWNPNVQRVRALVDGSP
                     KRLHVCTSCLKAGKVQRAV"
     gene            complement(17631..18179)
                     /locus_tag="VE646_13225"
     CDS             complement(17631..18179)
                     /locus_tag="VE646_13225"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZD80985.1"
                     /translation="MRVPRPPAILIVLCLGAGSCASSPPAAGSAIPSRPASNATTAAL
                     LPVDALSLPTFDVDRYRQLIDQLRGTPVVVNIWASWCGPCREEAPHLAAAARHLGASV
                     QFLGVDILDSRTSARSFIERYGWTYPSLFDETGAIRDQLGLIGQPATVFYDATGRVVS
                     TWSGAISADLLTRRLQQIRAGS"
     gene            18238..20523
                     /gene="recG"
                     /locus_tag="VE646_13230"
     CDS             18238..20523
                     /gene="recG"
                     /locus_tag="VE646_13230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HZD80986.1"
                     /translation="MALTLGSPIRAIDARLAGRRMGWKAKGPPASEVLADSLGIRTVG
                     ELLEHYPRRYIDRSQVTRIGDLRVGQQATVIAKVKEVKTGRTQRTHTDMATLKLYDGS
                     GYLSLVFFNQRWIPSRYRVGHELAVTGVVQRYRGTLQLGSQEVEVLRGDEQDLVHMAR
                     ITPIHPAAEHISTRTIRELIYRALEQLRAIPDPLPRSIAEAESLPELDGAVRRIHFPE
                     SQEELEGARERLKFDELFFLELGVAFRKHRLEAAEEGVAHRPDPELVRRLLATLPFEP
                     TGAQRRAMHRVGEAMARPRPMNLLLQGDVGAGKTLVAVHAALVAVGSRHQAAIMAPTE
                     VLAAQHLRSLMELLQPLGAVPYPPAAGRPPPPAVAGRSGHAGQLSLLEGPDGSGAGRA
                     EPHDLPGAGQRSTTGPALTYALLTAAVTGKERARILDGIANGEVDLVVGTHALVQEAV
                     TFHDLSLAVIDEQHRFGVHQRMALKGKGGSPDILIMTATPIPRTLALTYYGDLDVVVL
                     DEMPRGRRPVQTRIARTASERAQAYALVRREVERGRQAFVVCAAIDEANKREVRAAEA
                     EAERLASQVFPDLSVELLHGRMRPAQKERVMEEFRAGTHHLLISTTVIEVGVDVPNAT
                     VMLVENAERFGLAQLHQLRGRIGRAGYDSFCILFDESGGANEEARSRLEAMVRTTDGF
                     ELADEDLRLRGEGTLFDIRQSGMPDLRLARLAEDLDLVQRARARAFAVIGDDPRLEGH
                     PELVQELRSRFHRSIDWLFHS"
     gene            20576..21118
                     /gene="rsmD"
                     /locus_tag="VE646_13235"
     CDS             20576..21118
                     /gene="rsmD"
                     /locus_tag="VE646_13235"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HZD80987.1"
                     /translation="MIAGSARGTRLAPVPGGVRPMSDRAREGLFSSLGGAVRDAAVLD
                     LFAGTGALAIEALSRGAATAVLVERDRGALGTIRENLERTHLGDRARVVRAPVRRFLT
                     EPVNRAPFDLVLADPPYDVPARSVDGVLEALARGWLGPDWTAALTRRERSSMPVIPVN
                     WRLTRRLGYGDSLILVYRER"
     gene            21133..21618
                     /gene="coaD"
                     /locus_tag="VE646_13240"
     CDS             21133..21618
                     /gene="coaD"
                     /locus_tag="VE646_13240"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HZD80988.1"
                     /translation="MGLTALCPGTFDPVTNGHLDIIERACQHFDTVVVAVLENPAKQP
                     LFGLEERVGMLKEATADRPNVEIDAFSGLLVEFARRRGSQVIVKGLRAVTDFEYEIQM
                     AQMNRRLGSIETLFMTTDPNWSYLSSSLVKEVARFGGDVSGLVPSAVAERLAEKLGGA
                     A"
     gene            21618..22175
                     /locus_tag="VE646_13245"
     CDS             21618..22175
                     /locus_tag="VE646_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HZD80989.1"
                     /translation="MDLAARLQQLEDMVKEAKSMPLSSSALLNRDEVLELLADMKASL
                     PEEIKQARWVVRDREELLTKARRDSERLVDEGREEQLRLASQEQVVKRADEEAQRIVG
                     EAREEARQLRLEAEDYVDAKLAQFENALHRVSEELATTSSSLARTVDQVEVGRQKLHG
                     LAPAQQEFGPEEPLPSEGDDVEEAP"
     gene            22175..22696
                     /locus_tag="VE646_13250"
     CDS             22175..22696
                     /locus_tag="VE646_13250"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HZD80990.1"
                     /translation="MRAIDVRDLLERPGGSRSVRVEEPVEGLQTELADVPPDVPIAGD
                     LQLESVIEGIFVTGSLGGRMRLRCARCLKEFERGFDLQLNELVVREPGPDDDYGLGPD
                     LTLDPEPMVRDAVLLSMPFAPTCRPDCRGLCERCGGDRNLAECTCGPRVDPRWAGLEG
                     LDELFDDRSVDRS"
     gene            22714..22890
                     /gene="rpmF"
                     /locus_tag="VE646_13255"
     CDS             22714..22890
                     /gene="rpmF"
                     /locus_tag="VE646_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HZD80991.1"
                     /translation="MPTPKSRQSHARGAKRRANWKAQPPTYSECPQCHQPKLPHRVCS
                     NCGYYAGRQAIEVE"
     gene            22928..23620
                     /gene="rnc"
                     /locus_tag="VE646_13260"
     CDS             22928..23620
                     /gene="rnc"
                     /locus_tag="VE646_13260"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HZD80992.1"
                     /translation="MERALGVRFRDQDLRETALTHRSYAFEEGLGVNNERLEFLGDAV
                     LGLVVTDLAYREFPGLAEGELAKLRAAIVNMSALADVARDLDLGGAVKLGKGEEMSGG
                     RDKSSILADAMEAVLGAVYLDLGLGAARDLIERLFRPRMVAYVRGEGERDFKTILQEL
                     ASQDLRLLPEYRIEDRGPDHQKEFTATVYLGGDAWGTGVGRSKKEAEQQAAHEAYVRL
                     SERAPEDPPEGG"
     gene            23620..>24211
                     /locus_tag="VE646_13265"
     CDS             23620..>24211
                     /locus_tag="VE646_13265"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HZD80993.1"
                     /translation="MELPEVEVMRRDLDKDVVGRKIAKAEVRPTKNAMRAIRRHAKRK
                     EFTDRLIGRKIVKTERRGKYLLLHLDNGEALAVHFGMSGQFIRGNKRTPLPSHTQVVL
                     TFQQGGDLRFVDPRTFGELFVTSADDLGKVKELQHIGIDPLDDVLTWQTFGGLLAQHA
                     SKMKPLLMDQRFISGLGNIYSDEVLFAAGLRHDRMSD"
BASE COUNT         3286 a         8325 c         9082 g         3518 t
ORIGIN      
        1 cgatcgcgag tcccccgact cgttcccgaa ccccacggcc acgcggtcgt cgcgatgggc
       61 caacgcgcgc tcggcgtagt tcagctcggc gccggggaac cacacggcgc ccggcatccg
      121 ccgctcggcg aggacggcgt ccggggggcg gtgccaccgg accgagaaga actccgccac
      181 cgaggcccag aagccctcca gatcgtccac cgaccaggac cagagctcgg cgtagtcgcg
      241 gaacgaccgc ccccgggtct gctccaggaa ccgcaggtac cgagccaggt tcgaacgatc
      301 ccggcgttcg gcggacggct gccagaggat ctcgccctca gccaccatgc tcgaccatcg
      361 tagttcggaa cgcgcccgcg ttccgccggc cgaaccgcgt gggaccgctc gctccccgca
      421 ccgcgctagg ctgatccagg gatgcgcgag ggaggaggca tggccgaccg atcggacctc
      481 cagcggtacc gggagaacct gcgggacgag gtcgacagcg ccgagctgta cctggcgatg
      541 gccgagcggg aagcacaggc cgagctcgcc accctgtacc gcaagctggc cgacatcgag
      601 ctgcggcacg ccgggttctg ggaggagcga ctccgggagg ccggagcggc tgtgccggcg
      661 cggaggatca cctggcgggc ccgggtgctc cgatggctgg cgcgccggtt cggaccgcgg
      721 ctggtgctgc cgacggtggc atcgctggag gagatcaacc agcacatgta cgaccggcag
      781 cccgagacgg gcggaaccag cctgccggcc gacgagcgct cgcacgcccg gctcctgcgc
      841 acgttggccg gctcccccag gcggggcctg gagggcggaa ccctggccca ggtggagggc
      901 cggcaccggt cggtcggggg gaacgccctg cgggcggcgg tgctgggggc caacgacggg
      961 ctgacctcca acctcagcct ggtgatgggc gtggccggcg ccagctcgtc ctcgaccccg
     1021 gtggtggtcg ccggactgtc ggggctgttc gccggggcgt tctccatggc gctcggggag
     1081 tgggtctcgg tccagagctc gcgggagctc tacgagcggc agatggccgt ggaggccgag
     1141 gagatcgcca cgatccccga cgaggagcgc gaggagctga gcctgatcta ccaggccaag
     1201 ggcatgtccg cccaggcggc cgacgagttc gccgcccggc tgatgcagga tcaggcattg
     1261 gcgctggacg ccatggcccg cgaggagctc gggatcgatc ccgaggagct cggcggctca
     1321 ccgtgggtgg cggccttcgc gtccttcgcc ctgttcgccc tcggcgcggt gatcccgttg
     1381 gtgccgttcg tcctcgcctc gggcgccgca gccacggtgg cgagcctggt ggcctcgggg
     1441 gtgggactgt tcgcgatcgg cgtggcgatc acgctgctca ccggccgggg cgccctgctc
     1501 tcgggcgggc gccagctcgc gttcgggctg gcggctgcgg gcgtgacgtt cgcgatcggc
     1561 agcgcggtgg gctcggcgct cggctgaccg gcaggaccgc gcggggcggc gtacggctgg
     1621 gcagcgtcca ggaccgctgc tgctagcatc gggaccgagc attgggccgt ggggtaacgg
     1681 tagcccggtt ggctctggac caactagtcc aggttcgaat cctggcggcc cagctgagct
     1741 cttcgctgat gcgtccggat gcggtggcgg ttcgcgccca gcgcgtggta cttcgatctc
     1801 agcctgaagc gctctgtggg aacaccggca cgctcgccct cccggttgag gatcgggtgg
     1861 tctggatctg gccccgatgg ggaaagatcg tgccggacgg tacccgtgtg aggacggccg
     1921 ggtctgtcga cggcccgaga ggaggagcag gacgggagca ggccatcccg cacgatcgag
     1981 cgcgaacggc gacgggtggg tcgccgcctc ggagacgcgt tccccgcatt gaccagcgag
     2041 gatggcctcg ttacgttgca gggaccggca ggggaggcgg tgtcatgaac aagagcggct
     2101 tgatcgccga agtcgctaag cggacggggg cgagcaaggc ggagacgggg cggtccatcg
     2161 acgccacgat ggacgtcatc cgcgagacgg tggccaaggg cgatcgggtc tcgctggtgg
     2221 ggttcggcac gttcgagcgg aagcgccgga accggcgcat cgcgaggaac cctcggaagc
     2281 cccagacccc catcgtcgtg ccggccaggg atctgccctg gttcagcgcc ggcaaggagt
     2341 tccgggacat ggtcacgagc cgacgccggc gcacggccgc caagtccacc gcccgcgccg
     2401 ctagtcgaag gtgaccaggc gcagggtcct ggccacccgg cgcccgccct cctctaccag
     2461 atcgaacttc acccgctgac ccagccggag cgagcggatg ccggcgccct cgatggaggt
     2521 ggcgtcgacc tcgacctccg cgaggtcgtc cgtgagcacc gttcccgtgc gctgctcctc
     2581 gtcgtactcc ttgaccgtcc cctgcgccat ccgccctcct aggcatgcac cttcagccgc
     2641 tcggccaggt ccatctccag gcaaacctcc agggtccgtc cccggcgctc cgaggcgagc
     2701 acggtgagga tatccgaggg caggtccacg tcgaaggaca gctccgggcg ttcgaccacg
     2761 gaggccggga gccccctgag ctccgccaag gtcaggtgcc ggcggaacga atcggggccg
     2821 aaccgggcgg ggatgctcct gggcggccgc cggagcagca ggttggtgcc ctccccggtg
     2881 gaccggacca gcaccactgg tcccagcgtc cgcagcccct gcgcgagcgc gtcgggcgtg
     2941 atcaggggga ggtcgccggg gatgatggcc agggcggtcg cctcctcctc gagcgagagc
     3001 gcctccgcct gacgcaccgc cgaggagagc ggtggcttgg cctcggggac cggtcgcacc
     3061 tcccgcctgg cggcgatctc cagcaccacc tcgtcggggg agatcaccca cgtctcccac
     3121 cccggcaccg cggccgtggc gtccaggacg tcctcgagca tggccagggt cagcgcgcca
     3181 cgctccaggg gcgagagctc ggagcccaac cgcgacttgg tccgggagag ggacttgacg
     3241 gggatcgcga tcacgcgcac cactccaggc tagcgtcccg gggcggttgg ggcccggctt
     3301 tgcccacgga gggctcacag gtcgtagggt tggcgggccc atatcgaagg aggtgctcgt
     3361 gaccacgctc gccatcgccg cgtcgccggc ccggatccgg tcgagcgccg tctaccgcgt
     3421 gctcctcgcg ctggccggga gctggctggt ggccggcctc gcgcaggtgg aggtgcgcct
     3481 gccgttcacc ccggtccccg tcaccgggca gacgctgggg gtgctgctgg tgggggccgg
     3541 cctcggtccc gggctgggcg cgctctcggt ggggctgtac ctggcccagg gcgcggtggg
     3601 tcttccgttc ttctccggcg gggccggcgg agtctccttc ctcggggccg cggcggccac
     3661 cggcggctac ctgtgggggt tcctcctcgc cgcgaccgtg gtgggcttcc tggccaggcg
     3721 gggctgggac cgcacgcccg ggcgggccgt cgccgcgatg ctgctggggg aggcagtcat
     3781 ctacgccgtt ggtctcccct ggctgatggg gacggtggac gtgggtctgg cgcgagcgat
     3841 ctcgctcggg ttcacgccgt tcgtggcggg cgatctcctg aagctgctga tcgccgccgc
     3901 gctggtcccc gcggcctggc gggtggtccc gcgccggtag gatccgcgcc cggaagggag
     3961 gttgaggtgg aaccctggta ccgggtcgcc cggacggccc tgctgccgcc gatcggcctg
     4021 tggttcaact ggcgcctgga ggggctggag gcgatcccgc ccgagggtcc gctgatcgtg
     4081 gccggcaacc acctttcgta cctcgatccg ctggcccacg ggctgttcgt ggatcgggcc
     4141 ggccgccggc cgcgcttcct ggccaaacgc gagctgttcc agaacctgtt cgtgcggacc
     4201 gtgctgctgg gcgcgggcca gatcccggtg gatcggggga gcggtgccag ggctccgctg
     4261 atggacgccg cccgggcgct ggaggcgggg gaggtcgtgg tgctgtatcc ggaaggcacc
     4321 agcaccacca ccgatcccca gttctccccg ggccgcggga ggaacggcgc gatccgcctg
     4381 tcgctgatga ccggcgtgcc gatcctcccg gtcgcgacgt ggggcgggca gtacgtctgg
     4441 cgccgggggg agcggatccg ccccaccttc ggccgcccca tcctgatgac ggcgggcgcg
     4501 ccctacgatc cctcggcccg cagccgctcc gcggaggatc gaccgtcggt ccctcagctg
     4561 acggcggggc tcatgcggga gctctccggc ctggtggacg agcttcggac gcgctacccg
     4621 cggcgctgga ccccgggata ggctggggcg gatgagccag gagcgccgca gggggttcgc
     4681 cacccgggcg atccacgtcg cccccccggt ggtggatcag cagacgccga gcgtgcccat
     4741 ctaccagacc tccacgttcc ggttcgacac gtccgacgcg tacgccgaga cgatcgcgtt
     4801 ccgccgtccc ggatacacct acacgcgcgg gtacggcaat cccaccgtgc aggccttcga
     4861 ggagacggtg gccgacctgg aggggacctc gacggcgctc tccttcgcct cgggcatggc
     4921 cgcgatccac acgaccatca ccaccctggc cgggaggggg gatcgagtgg tggccggcag
     4981 ggagctctac ggggggacct actcgatgtt caccacggtc ctcccccggt acgggatcgg
     5041 ggtcgatctg gtcgatcccc acgacccctc cgcggtggca tccgcactgc ccggcgccgc
     5101 cgccttctac gtggagacga tcgccaaccc cacggtcacg gtggccgatc tccaggtgct
     5161 gggtgagctc tgcgccgagg ccggcgtgcc gtcggtggtg gacaacacct tcgcctcgcc
     5221 gtacctgtgc acccccgctc ggtacgggtt cgacctcgta ctccactccg ccaccaagta
     5281 cctgggcggc caccacgacc tgaccggcgg ggtggcctgc ggcagcgtgg agtcgatccg
     5341 acggctgcgc cagatggtga tcgagacggg ctcgtcgctc ggcccgttcg acgcctggct
     5401 agccctgcgg ggcctgatga cgctcgagct gcgcatgcag cggcacagcg cgagcgcggc
     5461 cgcgctggcc gcccacctgc agggccaccc gaggacggag gcggtccact acccggggct
     5521 ggcctcccac ccgcagcacg cggtggcccg gcgcatcctg ccgcgcggat ccggcgggat
     5581 gctcgccttc gaggtgaccg gcggggtgga ggcaggcaag cgcttctgcg acgccctccg
     5641 ggtggcctgg gtggccacca gcctgggggg cacgcacacc ctcgtggggc atgccgcttc
     5701 caccacccac cggcagatgg atcccgaggc gcgacgtgcg gcggggatcg gggacgggct
     5761 ggtccgggtg agcgtggggc tggaggacct ggacgacctg ctcgaggacg tcgaccaggc
     5821 gttggaggcg gcatgaggcg ccgggtcgcg atcgtgttcg gaggccgctc cgccgagcac
     5881 gagatctcct gcatctcggc ccgctcggtg atggatgccc tggatcctga tcgctacgag
     5941 atcgtgccga tcggcgtcac caaggacggt cgttggcagc tggtccccgg ggggcccccg
     6001 gccctgccgg ccgaccccgc caccgcgctg ccccaggtgg gcgatgcgtc gggacccgag
     6061 gtctcgctcg accaggagcc gggggcccgg gccctggtgg ccgaggacgg atcacgcacc
     6121 ccgatcgacg tggtcttccc cgtgatgcat ggaccctacg gtgaggacgg gtcgatccag
     6181 ggcttcctgg agatggccgg cgtcccctac gtgggggccg gcgtgctggg ctcggccgtg
     6241 gggatggaca aggcggtcca gaaggtcctc ttccgcgagg cgggcatccc cgtggtcgag
     6301 cacgaggtgg tccgggagcg cgagtggcag gaggaccggg aggcggtcgg ggcccgcgcc
     6361 gggcacctgg gctatccgat gttcgtgaag cccgcggcgc tcggctcctc ggtggggatc
     6421 aggaagatcc acggggtcca ggagctgtcg tccgccgtgg acgaagcgtt ccgctacggc
     6481 cgcaaggtgg tgctggagcg ctcggtggag ggagcccgcg agatcgaggt ggcggtgctc
     6541 ggcaacgacg atcccgtggc ctcggtggcc ggcgagatcg tgcccgcggg cggccacgag
     6601 ttctacgact acgaggccaa gtacgtcgac gagcacggag cgaacctgct ggtcccggcg
     6661 gagctacccc ccggggccct ggacgaggtg cagcgcctgg cgatcgcggg cttccgcgcc
     6721 gtcgagtgct cgggcatggc cagggtcgac ttcttcctgc tgagcgacgg gtccctgctc
     6781 ctgaacgaga tcaacacgat ccccggcttc accaggatct cgatgtaccc gaagctctgg
     6841 gaggcctccg gcctggccta ccccgcgctg atcgatcgcc tggtggagct ggcgctggaa
     6901 cggcacgcgg gcgagcgcaa gcggggaacc gtcctgggag ccgggtcggg gtcctcgccc
     6961 caggcccggg cggagggtcc gaacggtctc tagaggtgga ccaccgggca ggtcagggtt
     7021 cgggtgatcc cgtcgatcgc ctggatcttc gccaccacca gcttgccgag ctcgtccacg
     7081 ttcctggact cggcccgcac gatgacgtcg tagggccccg tcacgtcctc ggcctcggtc
     7141 acgccggcga tcccccggac ctcgtcggcc acctgcgctg ccttcccaac ctccgtctgg
     7201 atcaggatgt aggcatggac cacgtccgac ctccctcggt cctcgggccg caatgctatc
     7261 cgcgcccgct cgggcccggc aaaccgtccc cgcccgctcg ctgcggcggg accgacgatc
     7321 cgcccgtggt ggggacgctc ccgccccggg gggataatcg ggccatgggg agaggcaagg
     7381 acccgaagac ctccagcggc ttcctcgagc ggtacaggcg cgtgcgcaag cccatgccgc
     7441 caccggaacg ggtgatcttg gaccagcgcc gccgccgccg ggaggagcag gcccgtaggg
     7501 agatcgagga ggaggtcgac cggggctctg agaccgatga tgttcgccga tcgccgtgag
     7561 gcgggcgacc gcctggccca ggccctggag cgcttccggg gctcggacgc ggtggtgctg
     7621 gcgatccccc ggggcggggt gatcgtgggg gcggtggtgt ccgaggcgct cgacctgccg
     7681 ctggacgtgg tcgtcccccg gaaggtgggg gcgcccggca acccggagct cgggctgggc
     7741 gccgtcgccc cgggggtccg cgtgatcgac cctcggatgg tggaggccct cggcgtgggt
     7801 caggcgtacc tggagcggga gatcaccctc caagagcgag agatcgaacg gcggttggcc
     7861 gcgtaccggg ccggtcgtcc ggcggtcgcg gtacgcgatc gaaccgcgat cgtggtggac
     7921 gacggggtcg ccaccggcgg caccgccatc gccgcgctcc ggtgggctcg ggcgcagggg
     7981 gcgcacgagg tcgtgctggc cgtcccggtg gcccccggcg cctcgatccg gcgcttggac
     8041 cgggaggccg accgggtgct gatcctggcg gcccccgaac ccttctatgc ggtgggcgag
     8101 tggtacgggc ggttcgacca gacctccgac gagcaggtgg tggaggcgct ggcccgggcc
     8161 gcggagagcc ggacgtgagc gcggtcgagc tcggcatcgc caccgtgttg gccctggggg
     8221 ggatccgctc cctgtggcga tggacccgac ggcgcttcga gggcaccgat ctcgtggatc
     8281 acctgctcta cgccctgttc gtcaccggac gcgtgggtct gtggttctcc ctcgccgggc
     8341 tgttcctgat ctacgcggcc accacgatcg acggccggag cacgagcgcc ctggaccggt
     8401 tccgctggta cctgatcgtg cccatcgcca tggcggccgc gcagttcgcg gcgggctact
     8461 tcctgggcaa ccgcgccccg cgcgacgaga cgtgagcgag ctgttgcggg ggcgtgtcga
     8521 ccctgcgacg atcccgcgcg gatggtggcc gcgacgccga cgcggatccg tgggatgcta
     8581 gaacctagct atggcccgcg tgatccgctc ccggtccgca ctcgtcctcg gggccctcgt
     8641 gctggtgctg gcgctggtgg ccggcctggg ggccgtgcag gcggagcccc gcccggacct
     8701 gccgccggtg ggccccaagc gcctgatcgc ctcgaccctc gaggcgatcg cgcagcgggg
     8761 cccggtgtcg ggcgaggtcc acacccacgt ggacctgggc ctgccgcaac tccccgggaa
     8821 cctcgccgga ccctcgggcc cggccgacgt cctgctctcg gaccagacct tccgggtgtg
     8881 gcggtcggcc gacggtgtgc gcgtggcgca ggtcctgccc ttcggggagc gcgacgcggt
     8941 ggggaacgcc accgacgcct ggttctggga ctcgcagcgg ttcaccgcgt ggcacgcgtc
     9001 ggtcccggcg caggcgggct cgtcccccca gatcccctcg ctgggcgatc tgaccgacct
     9061 ggtgggcacg acgctccgcc ggctccagcc gtacgccgag gtccgggtga ccgacccggt
     9121 gatggtggcc gggcggagcg cgtacgtgct gcgcttggtc ccctcctccg gtgacacgct
     9181 gatcggtcac gtggacgtgg ccgtcgacgc tcggacccgg gtgccgctcc ggatctcggt
     9241 ggttgccgcc ggggggagcg cgccggtcat cgaggccggg ttcacctcgg tctcgttcgg
     9301 tccgatcgat ccccagatgt tctccttctc gccaccgcag ggcgcgaccg tgaagcaggt
     9361 ccgtccccac accggcatgg agcccgccgc tccggagcac ggggcgtcct cgggagacct
     9421 gccggccggc gtccgggtgt tcgggcgggg gttcgggacg gtcctggcga tccgggtcga
     9481 caccgtgccc caggacctcg gcccgctgtt cccgtacgcc ggcccgctgg ggtccgcgga
     9541 cgtcgtggag cgaggggacc acgcctggat cgtggccggc gccgtgccgc ccggccggct
     9601 ggccgaggtg gaaccgacgc tgccgtgacg gccctcgtgg cccgggggct gaccaagcgc
     9661 tacggaggcg tggacgtcgt ggacgggctc gacctccggg tggagcgcgg tgagctgtac
     9721 ggcttcctgg ggccgaacgg cgccggcaag accacgacga tccggatgtc gctgggcctg
     9781 atcttcccct cggccgggga ggtggagctg ctgggcaggc cggtgttcgg ggacggtcgc
     9841 tcgcaggccc tggcccgcac cggcgcgatg atcgaggagc ccggcttcta ccggttcctg
     9901 tccggacggc ggaacctgga ggcgttcgcc cgggccggcg ggggcgacgt gcgcgaccgc
     9961 ctgtcccgga tcgacgaggt gctggagctg accggtctgt ccggggccgc cggcaagcgg
    10021 gtgaaggcgt acagccaggg catgcgacag cggctcggca tcgcgctggc cctcctgggc
    10081 gagcccgaga tgctggtcct ggacgagccc acgaacggcc tggatccctc cgggatgcgg
    10141 gagatccgcc ggctcctgcg ccggttggcc gaccacggca ccacggtctt cgtctccagc
    10201 cacctgctct cggaggtgga ggcgatgtgc gaccgcgtgg gcgtgctgtc gcggggacgg
    10261 ctggtggccg agggtccccc cggcaagctg cgcggcaccg cggagaggct gcggatcgac
    10321 gtggacgatc gggaccgggc cctggccgtg ctggccgggg tgccgggggt cgaggcctcc
    10381 gccgacggag cgctggtgcg ggtgcgcctt cgcggcgccg agcccgccgc cgtgaacgcc
    10441 gcgctggtct cggccggcct ggccgtcagc gcgctggtcc ccgagcagga cacgctggag
    10501 gaggtcttcc tggcgttggt ggaggagggg catgtaccgg gttgagctgg cgaaggcggc
    10561 ccggcgctgg cggacctggc tggtggcggc cgccctgggc ggcatcccgg ccctgatcgt
    10621 gatcgcggtc aagctctccc cgcccgcgaa cgggggcggc caggacgcgc cgcccttcct
    10681 gatgcagatc gtccgcaacg ggctgttcgc tccgctcacc ggcctggccg tgatccagcc
    10741 gttcttcctg ccgctggcgg cggggctgtt cgccggcgac gcgatcgccg gcgaggcgca
    10801 gggcggcacg ctccgctacc tgctcgtgcg accggtgggt cgcgctcgcc tggtcctggc
    10861 caagtacgcg ggcgccatga ccctgctcgg tctgatgctg ctgtgggtgg tggcggtggg
    10921 catcgcgttc ggggcggtgg ccttcggcct ggggcccctg cccacgctct ccggctccac
    10981 cctgggcacc gggtccgcgg tgctccggat cgtgggggcc gccacgtaca tgttcctggt
    11041 ggtgtcgggg atcgtggcgg tgggcacctt catctcgacc ctcaccgact ccggaccggg
    11101 ggccaccgtg gccacggtga tcctgggcat cgccagcgag atcgtggaca acatcccgaa
    11161 cctgaaggtg gtgcatccgt acctgcccac ccacggatgg ctcgccttcg ccgatctgtt
    11221 ccggttcccc atcgcctggg gggcgatgtg ggacggggtg atcgtcagcc tggtctacac
    11281 cgtcgtcttc ctgggggcgg cgctgttgtg gttccggcgc aaggacgtcg cgtcctgagg
    11341 acgtccgggc gacgggggcc ccggggggga cgggcgccta gccgaccgcg cggacgatct
    11401 gctcggacag ctcccggaag ccctcgaggt gggcccgacg ctccgccctg aaccgctcca
    11461 cgaacccctg gtactcgcgc atgcgcccct gccgctcgta cagccggatg ggctggagca
    11521 gctccagatc ctcgatcccg ggcacggccg atgcgacctg ggtgccggag tcgtcgtcct
    11581 ggtcccaggt gatggtgccc tccgcgatgg ccttcaccac cgccgaggag tgggggatct
    11641 tcaccttctt cgagcgctcg tcgtcgggct tgccacccac ccggccggtg ttcagcagga
    11701 acacctcgat cgggtgggag cccaggagct ccaggatccg gttgccctgc aggccgtccg
    11761 gcatggggaa gaacgggttc gtcccgggaa cgcgcaggaa cttgccctcc tccgccttgc
    11821 cccccgcgga ggtgccggtg gtctcgccga gcatgaagta ggcggcggcc tgctcctggt
    11881 tcagcttgga gaccgccggg atgatgttct cgttccagtt gaggatcagc agaaagtcca
    11941 ccggtccgac cgcgcgcggg tccttgtgcc ggcccagcgc atccatgccg aacacggcgc
    12001 gcccgttctt cgtgtacgag gtctcgaaga agttcacgtt gccctgctgg tcctgataga
    12061 cgttctcgag atacgtgtcc gggctgatca ccgcgttgta gatgttgggc tcgaagtcgg
    12121 ggtcaaggct gaaggtcttc gcgaagcagc cgttctcggt cccgtaggtc ttgccgccgg
    12181 gcatcagcgc cacgaagtcg tcctgcaccg gcagggagct gttctgggtg gtgaacgtgg
    12241 tggtggtctt gccggtgccg gacagcccga tgatcaggaa caccttgtcg ccggccccgg
    12301 tgggcaccac cttgcagccc gcgtgcaggg acaggccgcc gcggtcgtag acgatgcgat
    12361 tccacatccg caggccgccc ttcttggact caccgaagta gtccgagttc agcacccggg
    12421 tcacgccgtt ctccaggtcc accgcgatca cgcggtcgtc cgggtacccc gggacgggca
    12481 ggttcggcgt gtagatgacc tgcacctcgg gctcgccccc ctcccgcggg aagtagagct
    12541 tctgctgcat cccggccacg ttggcgttgg cacgttcgat cgagagacga gccggcgtgc
    12601 ggaagtcggg atcgttgccg atgtacccgt cgatcaccag catctcctgc tgcgcgatgt
    12661 acgcgtcctg cagctcggcg atccgcgcgt actcctcgcg atccatcgac tgcccgccgg
    12721 cggtggaggg atcgtcggtc acgatgtagg tgctgcccac cgaccgggac gtggctcggg
    12781 tctggacgtt cacgttcccg aactcggtga ggcggcactg cggcatctcc tcggtgagct
    12841 tgcggagctc ctccggggat gggttgtcgt ggaggactcg ggctttgacg tgcaagggtg
    12901 atctccttcc gggatcatcc gctcgacgct cggatcgatc ctctctccgc tggcaggaac
    12961 tagattctac ccacggggcg gttcagaacc ggttcggccg cttcgcacag aagccgtgca
    13021 cggagcgtga cgaagcgtat ggagctgtcc gaggacgaac tgatcgcggc gatccgccgg
    13081 gtgctgtcgg ggcccggacc cggggtcgtg ctggggatgg gcgacgacgc tgcggtggtg
    13141 gaccgggggt cgggctcgct ggtgctcacc accgacatgc tggtggaggc cgtgcacttc
    13201 gaccgtgcgc tggtccccgc ccgggacctc ggggcgaagg cgatcgtggt caacgtctcg
    13261 gacatcgcgg cgatggcggc gagcccccgc tacgcgctgg tgtccctggc ggtaccggag
    13321 gacgtggggg ccaagtgggt ggtcgagctg gccggcggca tgcgcgacgc ctgcgaggag
    13381 tacgccctca cgctggtggg cggggacacc aaccgggccg acgaggtggt gatctcggtc
    13441 accgtggtgg gcgaggtggc ccccggacac cacatcaccc gcggcggggc ccatccgggc
    13501 gatctgatcg tggtgaccgg gacgttgggg gcggccgcgg ccgggtacct cctgagcgcg
    13561 atgccgccct cgcgggcggc acgcggactg tcgggcccgt gggggcggga tgcgctggat
    13621 gcgctgctcc acccggtggc ccgggtgggc gagggtcaga ccctggccca ggccggggcc
    13681 accgcgatga tggacctgtc cgacgggctg gccaaggacc tgtcccgcct gtgtgcggaa
    13741 agcggggtgg gggcccgggt ggaggcggcc cgcgtgccgg tctcccgggt ggtgcgcgag
    13801 ggggccgcgt tcctgggcgc cgatccgctg gagctggcga tcggcggggg agaggactac
    13861 gagttgctcg ccacgatgga tgcgaccgac ctggaccgcg cccgtgccga gctggacgag
    13921 cggttcggcg tgtcgctcac cgaggtgggg gtgatctcgg agtccggcct gacgctggtc
    13981 ggcgcggacg ggatcgaggg cccgctggcg ccgaagggat gggatcactt ccgccgtgcg
    14041 acctgacgct cgcgtccctc gggccctgac catcgccgga tcggactccg gcgggggagc
    14101 gggcatccag gcggacctga acacgttcgg cgcgctcggc gtgtacggga cgaccgcgct
    14161 caccgcggtc accgtgcaga acaccagggg ggtggcgggc taccaggagc tctctcctcg
    14221 gaccgtgggg gatcagatcc gggccgtcgc cgaggacatc gggctggacg cggccaagac
    14281 cgggatgctg gcctcgcggc cgatcgtggc ggcggtcgcc gacgccgtgg cccaggccga
    14341 ggtccccaac ctggtggtgg atccggtgtt cgtgtccaag cacggccact cgctcctggc
    14401 ccccgatgcg gtggaggccc tggtctcgcg gatccttccc ctggccaccc tcgtcacccc
    14461 gaacctgccc gaggctgccg gtctggccgg cttcggggtc accaccgccg aggacatgcg
    14521 ccgggccgcc gaggccatcc tccggctcgg cccttcggcg gtcctggtca agggcggtca
    14581 tcgcaccgac ggacgggccg acgatctgtt cctcgacggc gagcgcctcg tgtggatcga
    14641 ggggcagcgc atcgacgtgc gcaacacgca cggcaccggc tgcgtgctgt ccgcggccat
    14701 cgcggccgag ctggcccggg ggcgtgatct gctcaccgcg gtccggacgg ggaaggcctt
    14761 cgtgaccgag gccatccgcc acgcgctccc cctcggccac ggcatcggac cggtggaccc
    14821 gctgtggagg tcccggcagg gctgaggggc cccacggccc ggatcagtcc tcgcccagga
    14881 tgaagggtgc cgagtgcacc acgggatcgt cccgcgccac gaagtggagc acgtggcgcg
    14941 ccgagtcctt cttgccgggg accgtgagga agtacgcccg cttggacctc gagtagacga
    15001 agggcttgtg gagcggggaa ccgtcgatgt ccaccgcggt caccgacacg gaggacgagg
    15061 acacgttctt cccggccgcg tcgaccagcc ggatgatgat cttgcgtgag ggttcggacg
    15121 gccggtaccg gagctccacg ccgtaggtca ccacgtagcg gacgctcacg gtgccgacgt
    15181 tgcccacccg gtcgatcgcg gtcgccgtga acgagtgccg gcccacctcg ctggtgtcga
    15241 tcgatcccgg agccgagcac gaggccacgc cggagcccgc atcgacgcag gacaccgagg
    15301 cgtcgctggc ggcggactgg tgcagcagga gcacggcacc atcggcgatc ccggagagca
    15361 cgatccgggg cgcggcccgg tcgatccgga acccggccac cgggcccgcc gtggcgcagt
    15421 tgccgaccgc gtcgcagacg cggcgggtgc cggtccgggc gttcgcctcg gccctgccgg
    15481 ccggcacggc ggtggtcagc cggaacgagc gatccccggc tcgcaccccg gagccgccgt
    15541 cggtccccga gcaccgcacg gacccgttcg tggcctgcca ggcgtgcggt ggccgttcgc
    15601 aagaggctcc gggagccttg cggtcgaccc ggaggttctc gaacgggccc accgtggtgc
    15661 agttgccgac ggcgtcgcag acccggcgcg ctccggtccg ggccgcggcc gtctcgcggc
    15721 cacgctggat gctcgtgcga aggacgaagc tccgctccgc cgcggccagc cccgacccgc
    15781 cgtcgcggga cacgcagtgg acctgcacgt tcgcaccctg ccagaggtgc gtgggctgcc
    15841 ggcaggtcac ctcgggagcg cgctggtcca cgtggatccc gccgaagggg ccggcctgcg
    15901 cgcagttgcc ctcccggtcg cacaccctgc gggcatcggt ggaggcttcg gcatcctccg
    15961 accccggggc gacgtgcgtg ctcagctgga acgaggcgtc ggcggccgag gccaaccccg
    16021 agccggcatc ggacgccgtg cacgcgatca ggacgtcggc gccttgccaa ccgaggggcg
    16081 ggtcgtcgca gcggatccgc ggagcgtcgg tgtcgagcag ctggatgaac gcacccgtcc
    16141 cctccagcgc gaaggcgccc ggggccaccc cctccggtcc cgataccgtg agctccgcgg
    16201 tctggggtcc ggtcctggtg ggagagaaga tcacctggat ggcgcacgac ccttccgggg
    16261 tcagggtacg gccttcacag tcgccggacg gcacggagaa cgcgcccggg gcctccccgg
    16321 cgatccccac ctcggagatc tgcagggccc tatctcccac gttgagcacg gccaccgtct
    16381 gtggatcgct ggaggagccg accggtacct ccccgaaccc gccgtcaccc tggaattcga
    16441 tccgcggtcc cacctcgggc atcaggccgt ccaggatgct tcgcagcgac gggaggacct
    16501 cggtgggcac ctgggcgatc accggtcgca gcgacgaggt gacctgggcg ttgagagggt
    16561 cgaccggcac gccgcggtcg ctggcctgct gcaggttccg gaccagctcc tgggccaggg
    16621 cgggggtcgc ctcgaaccgg tggtcctcga tcgcgctgac cagttggatc agcgagctct
    16681 ggacctcctc ctgcaggggt gaggaagagc ccggggtggg cgcgaccggg ccgctggtgc
    16741 cgggcgtcgc cctcccggcg cggatcggct ggttcggtgc ggtcccgccc gacatgacca
    16801 ggagcacgcc tcccacgctg agggccaccg ccgcggcggc gacgaactgc agcacccgga
    16861 gacgtccggg tccccgcggc gcggccgacg ccccggtccc ggtgggggag gggacggccg
    16921 gctccacgag ctgagcggcg agggcggagg cgatggccga gagggaggag ccccgcggga
    16981 cgagggcatc ggccccttcc ggggcgttcc cggccgaggc caccacgatg atgcgggtgt
    17041 cgggcgacgc cgctcgcagg gcgtcgatcc gccctgcgtt cgcgatgtct ccgcccatca
    17101 cgacggcatc cggtcggtgc ttccggaccg ccgccaggag ctcttcgaag gtggcggcgt
    17161 gctcctgcac ctcgtagccg ctcccctcca ggaacggccg gacgccgccc acgctcaggt
    17221 agacgtcccc ccccgtgacg accgtgagtc gtccgtcgct cacccggtcc cctcctgctt
    17281 gtcccagaat cgtaccggcc accgaggcgc agacaagcgg tctttcggac cctcctggga
    17341 ggtcggtcgg gggccctggt cgggggtcgt tcggaccagg tcgggacggc tacgccacgg
    17401 tggccagccg ctcagaccgc ccgctggacc ttgcccgcct tcaggcagct ggtgcacacg
    17461 tgcaggcgct tcggcgagcc gtcgaccagg gcccggacgc gctgcacgtt cgggttccag
    17521 cggcggttgc tccgccgatg ggaatgggag acctgcttgc cgaagaacgg cttcttcccg
    17581 cagatgtcgc agacggctgc catggccgag acagggtagc agaggcccgg tcagctcccc
    17641 gcccggatct gctgcaggcg ccgggtcagc aggtcggccg agatcgcgcc gctccaggtg
    17701 gacacgaccc gcccggtcgc atcgtagaag accgtggccg gctgaccgat gagccccagc
    17761 tggtcgcgga tggccccggt ctcgtcaaac agcgatgggt aggtccagcc gtaccgctcg
    17821 atgaacgagc gggccgaggt cctggagtcc aggatgtcca cgcccaggaa ctgcacgctg
    17881 gcgccgaggt ggcgagccgc cgccgccagg tgcggcgcct cctcccgaca ggggccgcac
    17941 cacgaggccc agatgttcac caccaccggc gtcccccgca gctgatcgat gagctgccgg
    18001 taccggtcca cgtcgaaggt cggcagcgac agcgcgtcca ccgggagcag cgcggcggtg
    18061 gtggcgttgc tcgccggccg cgatgggatc gccgaccccg cggccggagg agaggaggcg
    18121 caggaccccg cgccgaggca cagcacgatc aggatggccg gagggcgggg gacacgcatg
    18181 agccccatgg tagaacccac ccatggtcat cacgcggcat ccgatcggtc caggcgcatg
    18241 gcgctgaccc tcggctcgcc catccgggcg atcgacgccc gcctggccgg ccggcgcatg
    18301 ggatggaagg cgaagggccc tccggcgagc gaggtgctgg ccgactcgct ggggatccgg
    18361 accgtcggcg agctcctgga gcactacccg cggcggtaca tcgaccgctc gcaggtgacc
    18421 cggatcggtg acctgcgggt ggggcagcag gccacggtca tcgccaaggt gaaggaggtg
    18481 aagacgggcc ggacccagcg cacccacacg gacatggcga ccctgaagct gtacgacggc
    18541 tcgggctacc tcagcctcgt gttcttcaac cagcggtgga tcccctcccg gtaccgcgtg
    18601 ggacacgagc tggcggtgac cggtgtggtc cagcggtacc gaggcaccct gcagctcggc
    18661 agccaggagg tcgaggtgct gcggggcgac gagcaggacc tggtccacat ggcccggatc
    18721 accccgatcc atccggccgc cgagcacatc agcacccgga cgatccgtga gctcatctac
    18781 cgggccctcg agcagctccg tgcgatcccc gaccccctgc cccggtcgat cgcggaggcc
    18841 gagtccctgc ccgagctgga cggcgccgtc cggaggatcc acttccccga gtcccaggag
    18901 gagctggagg gggcgcgcga gcggctgaag ttcgacgagc tgttcttcct cgagctcggc
    18961 gtggccttcc gcaagcaccg cctggaagcg gccgaggagg gggtggcgca ccgccccgat
    19021 cccgagctgg tccgccgcct cctggccacc ctgcccttcg agcccacggg ggcccagcgc
    19081 cgagcgatgc accgggtggg ggaggcgatg gcccgtcccc gtcccatgaa cctattgctg
    19141 cagggcgacg tgggcgccgg gaagacgctg gtcgcggtgc acgcggcgct cgtggccgtg
    19201 ggctcgcgcc accaagcggc gatcatggct cccaccgagg tgctggccgc tcagcacctg
    19261 cgatccctga tggagctgct ccagcccctg ggcgccgtgc cgtatccgcc tgccgccggc
    19321 cgtccccctc cgccggcggt cgccgggcgg tcgggccacg ccgggcagct ctccctcctg
    19381 gagggtcccg acgggtccgg tgccggtcga gccgagcctc acgaccttcc cggagccggg
    19441 cagcggtcga ccaccgggcc ggcgctcacc tacgcgctgc tgacggcggc ggtgacgggc
    19501 aaggagcgag cgaggatcct ggacgggatc gcgaacggtg aggtcgacct ggtcgtgggc
    19561 acccacgccc tggtccagga ggcggtgacc ttccacgacc tgtccctggc ggtgatcgac
    19621 gagcagcacc ggttcggcgt gcatcagcgg atggcgctga agggcaaggg cggctcgccg
    19681 gacatcctga tcatgacggc gacgcccatc ccgcggacgt tggccctcac ctactacggg
    19741 gacctggacg tggtggtgct ggacgagatg cccaggggac gtcggccggt ccagacccgg
    19801 atcgcgcgca ccgcgtcgga gcgggcccag gcctacgcgc tggtgcgccg ggaggtggag
    19861 cgtggccggc aggcgttcgt ggtgtgcgcg gcgatcgacg aggcgaacaa gagggaggtg
    19921 cgtgccgccg aggcggaggc cgagcgcctg gcgtcccagg tgttccccga cctgtcggtg
    19981 gagctgctgc acgggcggat gcgaccggcg cagaaggagc gggtgatgga ggagttccgg
    20041 gccgggaccc accacctgct gatctccacg acggtgatcg aggtgggggt ggacgtcccg
    20101 aacgccaccg tgatgctggt ggaaaacgcc gagcgcttcg gcctggccca gctgcaccag
    20161 ctccgaggaa ggatcggccg ggccgggtac gactcgttct gcatcctgtt cgacgagagc
    20221 ggcggcgcca acgaggaagc gcgctcgcgc ctggaggcca tggttcggac caccgacggg
    20281 ttcgagctcg ccgacgagga cctgcggctg cgcggggagg gcaccctgtt cgacatccgg
    20341 cagtcgggga tgcccgacct gaggctcgcc cgcctggcgg aggacctcga tctggtccag
    20401 cgggcccgcg ctcgcgcctt cgcggtgatc ggggacgatc cgcggctcga agggcacccc
    20461 gagctggtgc aggagctgcg atcgcggttc caccgctcca tcgattggct cttccattcc
    20521 tgaggccggg cacgcccggg ggtccaccgt gcggctagca tgccgctcgg tgcgcgtgat
    20581 cgccggttcc gccaggggca cacggctggc gccggttccc ggaggggtcc ggccgatgtc
    20641 cgaccgcgcg cgggagggcc tgttctccag cctgggtggg gcggtccggg acgcggccgt
    20701 cctggacctg ttcgcgggca ccggggccct ggccatcgag gccctgtcca ggggggcggc
    20761 cacggccgtc ctggtggagc gggaccgggg ggccctgggg acgatccggg agaacctcga
    20821 gcggacgcat ctcggcgatc gggcgagggt ggtccgcgct ccggtgcgac gattcctcac
    20881 cgagcctgtc aaccgagccc ccttcgacct cgtgctggcc gatccgccct acgacgtccc
    20941 cgcccgcagc gtggacggcg tgctggaggc cctggcccgg ggctggctgg gtccggactg
    21001 gaccgccgcc ctcacgcgca gggagcggag ttccatgcct gtcattccgg taaactggcg
    21061 gctcacgagg cgtctcgggt acggagacag cctcatcctg gtctaccgcg agcgttagcg
    21121 ggggaggtcc gcatgggact gaccgccctg tgtccgggga cgttcgaccc agtgacgaac
    21181 ggtcatctcg acatcatcga acgtgcttgc cagcacttcg acacggtggt ggttgcggta
    21241 ttggagaacc cggccaagca gccgctgttc ggtctcgagg agcgggtcgg catgctgaag
    21301 gaggccacgg ccgaccggcc caacgtggag atcgacgcct tctccggatt gctcgtggaa
    21361 ttcgcaaggc ggcggggctc gcaggtgatc gtgaaggggc tccgggccgt caccgacttc
    21421 gagtacgaga tccagatggc gcagatgaac cgacgactgg ggtcgatcga gaccctgttc
    21481 atgaccacgg acccgaactg gtcctacctg tcctcctcgt tggtcaagga ggtggcccgg
    21541 ttcgggggag acgtctcggg actggtcccg tccgcggtcg cggagcggct ggccgagaag
    21601 ctggggggag cggcttgatg gacctggccg cccgactgca gcagctggag gacatggtga
    21661 aagaggccaa gtcgatgccg ctgtcctcct cggccctcct gaaccgcgac gaggtgctcg
    21721 agctgctggc cgacatgaag gcatcccttc cggaggagat caagcaggcc cgctgggtgg
    21781 tgcgcgatcg cgaggagctg ctgaccaagg cccgccgcga ttcggagcgg ctggtggacg
    21841 aggggcgcga ggagcagctg cggctggcct cccaggagca ggtcgtgaag cgcgcggacg
    21901 aggaggccca gcggatcgtg ggcgaagccc gcgaggaggc ccgccagctg cgcctggagg
    21961 ccgaggacta cgtggacgcc aagctggcgc agttcgagaa cgcgctgcac cgggtgagcg
    22021 aggagctggc gaccaccagc agcagcctgg cccgaaccgt ggatcaggtc gaggtggggc
    22081 gccagaagct ccacgggctg gccccggcgc agcaggagtt cggcccggag gaacccttgc
    22141 ccagcgaagg cgatgacgtg gaggaggcac cgtgatgcgc gcgatcgacg ttcgcgatct
    22201 gctcgagcgt ccggggggct cgcgctcggt ccgcgtggag gagccggtcg agggcctcca
    22261 gaccgaactg gccgacgtcc cccccgacgt ccccatcgcc ggggacctcc agctggagag
    22321 cgtgatcgag gggatcttcg tcaccggctc cctcggcggc cgcatgcgcc tgcgctgcgc
    22381 acgctgcctg aaggagttcg agcgcgggtt cgacctgcag ctgaacgagc tggtggtccg
    22441 ggagccgggt cccgacgacg actacggcct cggtccggac ctcacgctgg accccgagcc
    22501 catggtccgt gacgcggtcc tgctgtcgat gccgttcgcc cccacctgcc ggcccgactg
    22561 ccgagggctc tgcgagcggt gcgggggcga ccggaacctc gccgagtgca cctgtggacc
    22621 ccgtgtcgac ccccgatggg cggggctgga gggcttggac gagctgttcg acgaccggtc
    22681 ggtcgaccgg tcctgaccgg aagggaccca gggatgccga caccgaagag caggcagagc
    22741 cacgcgcgcg gcgccaagcg tcgtgccaac tggaaggccc agcctcccac ctattccgag
    22801 tgcccgcagt gccaccagcc gaagctcccc catcgggtgt gctcgaactg tgggtactac
    22861 gcgggccgac aggcgatcga ggtcgagtag ccgcgtgatc gagcccgggt ccgacgagcc
    22921 cggcgacctg gagcgtgcgc tcggcgtccg gttccgggac caggacctgc gcgagaccgc
    22981 gctcacccac cgctcctacg ccttcgagga gggcctgggc gtcaacaacg agcggctgga
    23041 gttcctgggc gacgcggtgc tcggcctggt ggtgaccgac ctggcgtacc gggagttccc
    23101 cgggctggcc gagggcgagc tggcgaagct ccgcgcggcg atcgtgaaca tgtcggccct
    23161 ggccgacgtg gccagggacc tggatctggg cggcgcggtc aagctcggca agggcgagga
    23221 gatgagcggt gggcgggaca agtccagcat cctggccgat gcgatggagg cggtgctcgg
    23281 agcggtctac ctggacctcg gcctcggggc ggcccgcgac ctgatcgagc ggttgttccg
    23341 cccgcgcatg gtggcctacg tccggggcga gggggagcgc gacttcaaga cgatcctgca
    23401 ggagctggcc tcgcaggacc tccggctgct gcccgaatac cggatcgagg atcgcggccc
    23461 ggaccatcag aaggagttca ccgccacggt gtacctgggg ggcgacgctt gggggaccgg
    23521 cgtgggccgc tcgaagaagg aggccgagca gcaggcggcg cacgaggcgt acgtgaggct
    23581 ctcggagagg gcgccggagg acccgccgga ggggggatag tggagcttcc cgaagtcgaa
    23641 gtgatgcgcc gagacctgga caaggacgtg gtggggcgca agatcgcgaa ggccgaggtc
    23701 cggcccacga agaacgccat gcgcgcgatc cgccgccacg ccaaacgcaa ggagttcacc
    23761 gatcggctca tcggtcgcaa gatcgtgaag accgagcggc gcggcaagta cctgctgctg
    23821 cacctggaca acggcgaagc gctcgccgtg cacttcggca tgagcgggca gttcatcagg
    23881 gggaacaagc ggacgccgct gccctcgcac acgcaggtgg tcctgacctt ccagcagggg
    23941 ggcgacctgc gcttcgtcga cccgcggacc ttcggggagc tgttcgtcac ctcggcggac
    24001 gacctgggca aggtcaagga gctccagcac atcgggatcg accctctgga cgacgtcctc
    24061 acctggcaga cgttcggggg gctgctggcg cagcacgcct ccaagatgaa gcccctgctg
    24121 atggatcaga ggttcatctc cgggctgggc aacatctact ccgacgaggt gctcttcgcc
    24181 gccggactgc gccacgaccg gatgtcggat t
//
LOCUS       DASQQQ010000357         7121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_272761, whole genome shotgun sequence.
ACCESSION   DASQQQ010000357 DASQQQ010000000
VERSION     DASQQQ010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272761"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VE646_13270"
     CDS             <1..343
                     /locus_tag="VE646_13270"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZD80994.1"
                     /translation="RLPAPPREVWWALTSPERLAAWFGAEVAIEPRAGGPVEVRWADG
                     TRARGVVEEALEPRRLVLRWRRLSGAGLAMAVGPATRIAFELRPRVDGTDLAVEEVPV
                     ELVSAPGGWGA"
     gene            340..672
                     /locus_tag="VE646_13275"
     CDS             340..672
                     /locus_tag="VE646_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZD80995.1"
                     /translation="MSQVDAVFAALSDRTRRSVIRSLSERGPTTATELAGELPVTRQA
                     VSKHLAALEEAGLVSSSVRGRSRRYRLTPGPLAEAMGWMADVGAQWDDRLEALRRHLM
                     TGGPEGRR"
     gene            730..1203
                     /gene="nrdR"
                     /locus_tag="VE646_13280"
     CDS             730..1203
                     /gene="nrdR"
                     /locus_tag="VE646_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HZD80996.1"
                     /translation="MRCPWCGHEEDKVVDSRPADRGSAIRRRRQCLSCGRRYTTFERI
                     EDVGLVVAKRDGSREPYLREKVVSGIRKAIVNRPVSEDQVARVVQRVEERLRRKGPDL
                     TSQQVGFEVLSELKKLDQVAYIRFASVYKGFQELTDFERELGQLLKKVPAKPRNR"
     gene            1376..6493
                     /locus_tag="VE646_13285"
     CDS             1376..6493
                     /locus_tag="VE646_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HZD80997.1"
                     /translation="MATNEGDVEVIRQGLAFKRYFTKDGVHPFDEVEWEIRDAVIPNF
                     KEGGNAFEQRGVEFPKGWSQNATNIVAQKYFRGPLGMPQRESSVRQLIGRVVDTIRGW
                     GLKDGYFASANDADVFAEELSHLVVTQKAAFNSPVWFNVGVPDTPQQCSACQPHDALV
                     STPSGLIPIGELVERNAVGTKVFDGNGVTRILAVKANGRKEVLRIHTKAGHALDVTAD
                     HLVWRSSHSGSGRFVEAGTLRPGDLLEWHRHDAYGEGEVDRRQIAEAALAGWLQSDGC
                     VGQYEGANRSLTIEAMTVNRWEHEWVLDALDTVFSDVHRHERTVETQDRSLDCRRVRL
                     YGEPLRDFVNRWGLLTRGVDMRVPRQLFTAPQPIVAAYLRSLFQAEGYVSVRVSSGRI
                     GLDMISEDLVRDVQQLLGRFGIFSRVGFKSDRRPDREGCWSLSIANVGDQVTFAEEIG
                     FLDPRKTDRLEMALDRVGRTTRSVKQLQIARIENLGTMEVYDIQTESGEYLSGGLRVH
                     NCFILSVDDTMSSILNWYVEEGTIFKGGSGSGINLSKIRSSKEPLGGGGTASGPVSFM
                     RGADASAGTIKSGGKTRRAAKMVVLNADHPDVRDFIWCKANEEQKARALRDAGFDMDL
                     DGKDSYSVQYQNANNSVRVTDEFMQAYLDDRDWKLKAVTTGETIETVRARDLMREVAQ
                     AAWECADPGMQYDTTINEWHTCPASGRINASNPCSEYMHLDNSACNLASLNLMRFVDV
                     DGNFDVAAFRHAVEIVFTAQEIVVGNADYPTESIGRNTKAFRQLGLGYANLGALLMAR
                     GLPYDSDGGRAWAGAITALMTGWAYRTSARIAEVTGPFAGFAPNRDAMLRVVRKHRAA
                     ADDIDGELVPEALLSAAKQAWDEAVGLGEQHGYRNAQASVLAPTGCLVGGSLVPTERG
                     LVRLGTLGDPAGDRWQELGLRVDTDEGPRRATKFYVNGVEAVVSVGTSRGFRIQGTPR
                     HRIKVVREDGSWEWKRFAELGEGDLVPLALDQLIGQPQPVELPPMPEAHWTGERHAVA
                     PRRMDPELAELVGYFMGDGSLHARGIRLWVGPSDLDVVERLRQLAKSLFGLEAQVTER
                     GGYTEVAFNSVRLVLWWEACGFAKREPVPGRSGKGYAPHVPDAVLHSNDREVYAAFLR
                     GLFEADGTVTAGCPHWSTTSLEFSHDVQTVLLALGYPTTRKMDVTGWGRSPLAVLRLL
                     NASYGGRWLEEIGFIADRKNAAVHTPGGGRAARADRIPMTRDMIDRLAPANDRLRKVL
                     LMETGRTGMVSRRIATELFGRTQDPALGHVLRFFYDRVTSAELGDDELTYDLSVPENL
                     TYIANGFVSHNTIGLMMDCDTTGIEPDLALVKNKKLVGGGTMQIVNQTVPRALERLGY
                     QADQVDDIVGYISERNAVVDAPHLREEHYPVFDTSMGERSIHYMGHVRMMGAVQPFIS
                     GAISKTVNMPEEATVEEVEQLFAESWRLGLKAVAIYRDNCKVAQPLSADRKTPAAEPV
                     ELATPVRRRLPQSRPSLTTKFEVGDAEGYITASSYPDDGIGEIFLKTSKQGSTLSGIT
                     DAFSIAISIGLQYGVPLETYVQKFINMKFEPSGMTNDPDIRFATSIVDYVFRRLALDH
                     LSKEQREQLGIWAISERREMAAAEAAEKLNGGVPAPSAEAPLGEGPGSSPARAEAVAR
                     PEIRAADAPLCYQCGSKMQPAGSCYVCPTCGSTSGCS"
     gene            6542..6976
                     /gene="dut"
                     /locus_tag="VE646_13290"
     CDS             6542..6976
                     /gene="dut"
                     /locus_tag="VE646_13290"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HZD80998.1"
                     /translation="MDLPFKRLDPDAELPGPAHPDDAGLDLRSTVDVEIGPGERALVP
                     TGVAVAIPPGHAGLVLPRSGLASKQGLTMANAPGLIDAGYRGEVICAVVNLDRGHAVR
                     LARGDRIAQLVVVPVPPARPMWVEELPPSARGAGGFGSTGSG"
BASE COUNT         1113 a         2358 c         2601 g         1049 t
ORIGIN      
        1 ccgcctgccg gcgcccccgc gcgaggtgtg gtgggcgctg acctcgcccg agcggctggc
       61 ggcctggttc ggagccgagg tcgcgatcga gccccgggcc ggtgggccgg tggaggtgcg
      121 ctgggccgac ggcacccgcg cccgcggggt ggtggaggag gccctcgagc cccgtcggct
      181 cgtgttgcgg tggcgacgtc tcagcggggc cggcctcgcg atggcggtgg gcccggccac
      241 ccggatcgcc ttcgagctgc ggccccgcgt ggacggaacc gacctggccg tggaggaggt
      301 tccggtggag ctggtctcgg cgcccggcgg gtggggagca tgagccaggt ggatgcggtc
      361 ttcgccgcgt tgtccgatcg cacccggcgc tccgtgatcc gctcgctgtc cgagcgtggc
      421 ccgaccacgg ccacggagct ggcgggcgag ctgccggtga cccgccaggc cgtctcgaag
      481 cacctggccg cgctggagga ggccggcctg gtgagctcgt ccgtccgggg gcggagccgc
      541 cggtaccggc tgacccccgg acccctggcc gaggcgatgg gctggatggc cgacgtgggg
      601 gcccagtggg acgatcggct ggaggcgctg cggcggcacc tgatgacggg agggcccgag
      661 ggccggcgtt gacgctgcaa catctggcgg ctaccgtgcc atcgaccact acatgttgtg
      721 ggtgatgcca tgcgatgccc gtggtgcgga cacgaagagg acaaggtggt ggactcccgg
      781 cccgcggacc gggggtcggc gatccgtcgc cggcgccagt gcttgtcctg tgggcgtcga
      841 tacacgacct tcgagcggat cgaggatgtc ggcttggtgg tggccaagcg cgacgggtcc
      901 cgcgagccct acctgcgcga gaaggtcgtc tcggggatcc gcaaggccat cgtgaaccgc
      961 ccggtgtccg aggaccaggt ggcccgcgtg gtccagcggg tggaggagcg cctgcggcgc
     1021 aaggggccgg acctgacctc ccagcaggtg ggcttcgagg tgctctccga gctgaagaag
     1081 ctcgatcagg tcgcgtacat ccggttcgcc agcgtctaca agggcttcca ggagctgacc
     1141 gacttcgagc gcgagctcgg ccagctgctc aagaaggtcc ccgccaagcc ccgcaaccgc
     1201 tgaccaccgc ctcggcccct ccgcatcggt cgagatacaa tatcttgtgg tgcggcggaa
     1261 ccaagaccac aagatatccg gtccgacccc cttgacgcga gggcccgccg ggtagagaat
     1321 cccaaagctg tcattcatga cttccccacg gatccacgga aagggggctt tccctgtggc
     1381 gacgaacgag ggggacgtcg aggtcatccg gcagggcctg gcgttcaagc gatacttcac
     1441 gaaggacggg gtccatccct tcgacgaggt cgagtgggag atccgggacg ccgtcatccc
     1501 gaacttcaag gagggcggca acgccttcga gcagcgcggc gtcgagttcc ccaagggctg
     1561 gtcccagaac gcgacgaaca tcgtggcgca gaagtacttc cgcggccccc tcggaatgcc
     1621 acagcgtgag tccagcgtcc gccagctgat cggccgggtc gtggacacca tccgcggctg
     1681 gggcctgaag gacggctact tcgcctccgc gaacgacgcc gacgtgttcg ccgaggagct
     1741 gagccacctc gtggtcaccc agaaggcggc gttcaactcg ccggtgtggt tcaacgtcgg
     1801 ggtgccggac acgccccagc agtgctcggc gtgccaaccc cacgacgcgc tggtcagcac
     1861 gccgtctggc ctgatcccca tcggcgaact ggtcgagagg aacgcggtcg ggacgaaggt
     1921 gttcgacggg aacggcgtga cgcgcatctt ggcggtcaag gcgaacggtc gcaaggaggt
     1981 cctgcgtatc cacaccaagg cgggacatgc gctcgatgtg acggccgatc acctcgtgtg
     2041 gaggagctcg cactcgggct cgggacgctt cgtcgaggcg gggacgctcc gtcccggtga
     2101 cctcctggag tggcaccgcc acgatgccta cggggagggg gaggtcgatc ggcgtcagat
     2161 cgccgaagcg gcgctcgccg gttggctcca gtcggacggg tgcgtcggcc agtacgaggg
     2221 cgcgaaccgc tccctgacca tcgaagccat gacggtgaac cgatgggagc acgagtgggt
     2281 cctcgatgcc ctcgacacgg tgttctccga cgtgcaccgc cacgagcgaa cggtcgagac
     2341 gcaggatcgc tcgctggact gccgcagggt gcgtctgtac ggggagccgc tccgggactt
     2401 cgtgaaccgg tggggcctgc tcacccgggg cgtggacatg cgggtccccc ggcagctgtt
     2461 caccgctccg cagccgatcg tcgccgccta cctgagaagc ctgttccagg cggagggcta
     2521 cgtgtcggtc cgggtttcat ccggccgcat cgggctcgac atgatctccg aggacctggt
     2581 ccgagacgtc cagcagctcc tgggccgctt cgggatcttc tctcgggtgg ggttcaagtc
     2641 cgatcgccgg ccggaccggg aggggtgctg gtcgctgtcc atcgccaacg tcggcgacca
     2701 ggtcacgttc gcggaggaga tcgggttcct cgatccccgg aagaccgacc ggctggagat
     2761 ggcgctggac cgcgtgggcc ggaccaccag gagcgtgaaa cagctccaga tcgcgcggat
     2821 cgagaacctc ggaacgatgg aggtctacga cattcagacc gagagcgggg aatacctctc
     2881 gggaggcctc cgcgtgcaca actgcttcat cctctcggtc gacgacacca tgagctcgat
     2941 cctcaactgg tacgtggagg aggggaccat cttcaagggc ggatcgggct cggggatcaa
     3001 cctgtccaag atccgctcgt ccaaggagcc gctgggcggc ggcggcaccg cctcggggcc
     3061 ggtcagcttc atgcggggcg ccgacgccag cgccggcacg atcaagtcgg gcggcaagac
     3121 ccggcgggcc gccaagatgg tcgtgctgaa cgccgatcac cccgacgtgc gcgacttcat
     3181 ctggtgcaag gccaacgagg agcagaaggc gcgcgccctg cgcgacgccg gcttcgacat
     3241 ggacctggac ggcaaggaca gctactcggt ccagtaccag aacgccaaca actcggtccg
     3301 cgtcaccgac gagttcatgc aggcctacct ggacgaccgg gactggaagc tcaaggccgt
     3361 gaccaccggc gagaccatcg agaccgtgcg ggcccgcgac ctgatgcgtg aggtggccca
     3421 ggcggcgtgg gagtgcgccg accccggcat gcagtacgac accaccatca acgagtggca
     3481 cacctgcccg gcctcggggc ggatcaacgc gtcgaacccg tgcagcgagt acatgcacct
     3541 ggacaactcg gcctgcaacc tggcgtcgct gaacctgatg aggttcgtgg atgtcgacgg
     3601 caacttcgac gtggccgcct tcaggcacgc cgtcgagatc gtgttcacgg cccaggagat
     3661 cgtcgtgggg aacgccgatt accccaccga gtcgatcggc cgcaacacca aggccttccg
     3721 ccagctgggg ctgggctacg cgaacctcgg cgcgctgctg atggcccgag ggctgcccta
     3781 cgactccgac ggcggtcggg cctgggccgg ggccatcacc gcccttatga ccggctgggc
     3841 ctaccggacc agcgcgcgga tcgccgaggt gaccgggccg ttcgcgggct tcgcgccgaa
     3901 ccgcgacgcg atgctccggg tggtccgcaa gcaccgcgcc gcggccgacg acatcgacgg
     3961 cgagctggtg ccggaggccc tcttgtccgc ggccaagcag gcctgggacg aggcggtggg
     4021 cctgggggag cagcacgggt accgcaacgc ccaggcctcg gtgctggctc ccaccggttg
     4081 cctcgtgggg ggctccctgg tgcccaccga gcgaggtctt gtgcgccttg gcaccctggg
     4141 tgatccggct ggcgatcgct ggcaggaact gggtctccga gtggataccg acgaagggcc
     4201 tcgtcgtgcc accaagttct acgtgaatgg ggtcgaagcg gtggtgagcg tcgggacatc
     4261 ccgcggcttc cgcatccagg ggaccccgcg tcatcggatc aaggtggtcc gcgaggacgg
     4321 ctcctgggag tggaagcggt tcgccgagct cggcgaaggg gacctggtcc cgctcgccct
     4381 cgatcagctc atcggccagc cacagccggt cgaacttcct cccatgcccg aggcccactg
     4441 gacgggggag cggcacgcgg tcgcaccccg gcgcatggat ccggagctgg cggagctcgt
     4501 cgggtacttc atgggtgacg gttcgctcca tgcccgcggc atccgtctat gggtcggccc
     4561 gagcgatctc gacgtcgtgg agcgcctgag gcagttggcg aagtcgctgt tcggcctgga
     4621 ggcgcaggtg acagagcggg ggggctacac ggaggtcgcc ttcaactcgg tccgactcgt
     4681 cctgtggtgg gaggcgtgtg ggttcgccaa gcgcgagccg gtccctggcc gttccggcaa
     4741 ggggtacgca ccgcacgtgc cggacgccgt cctgcacagc aacgaccgcg aggtctacgc
     4801 ggccttcctc cgcgggctgt tcgaggccga tgggaccgtc acggccggat gtccgcactg
     4861 gtcgaccaca tcgctggagt tctctcacga cgtgcagacc gtgctgctgg cactcgggta
     4921 ccccaccacc cggaagatgg acgtcacggg atgggggcgc tcgccgctgg cggtcctgcg
     4981 gttgcttaac gcctcgtacg gtgggcgctg gctcgaggag atcggtttca tcgccgaccg
     5041 gaagaacgcc gcggtccaca caccgggcgg tggtcgggcc gctcgggcgg acaggatccc
     5101 gatgacccgc gacatgatcg accggctcgc gccggcgaac gaccggttgc gcaaggtcct
     5161 gctcatggag acgggccgca cggggatggt ctcgcggagg atcgcaactg agctgttcgg
     5221 gcggacccag gaccccgcgc tggggcatgt gctgcggttc ttctacgatc gcgtgacctc
     5281 ggccgagctc ggcgacgacg agctgacgta cgacctgtcg gtgccggaga acctcaccta
     5341 catcgcgaac ggcttcgtga gccacaacac catcggcctg atgatggact gcgacaccac
     5401 cggcatcgag cccgacctgg cgctggtgaa gaacaagaag ctggtgggcg ggggcacgat
     5461 gcagatcgtg aaccagaccg tgccgagggc cctggagcgc ctggggtacc aggcggacca
     5521 ggtcgacgac atcgtcggct acatcagcga gcgcaacgcc gtggtcgacg ccccgcacct
     5581 tcgcgaggag cactacccgg tgttcgacac ctcgatgggc gagcggtcga tccactacat
     5641 ggggcacgtg cggatgatgg gcgcggtcca gcctttcatc tccggcgcca tctccaagac
     5701 cgtgaacatg ccggaggagg ccaccgtgga ggaggtcgag cagctgttcg cggagagctg
     5761 gaggctgggg ctcaaggccg tggcgatcta ccgtgacaac tgcaaggtgg cccagcccct
     5821 gtcggcggac cgcaagaccc cggcggccga gcctgtcgag ctggccacgc cggtgcgcag
     5881 gcgcctgccg cagagccgcc cctcgctcac cacgaagttc gaggtggggg acgccgaggg
     5941 ctacatcacc gccagctcct acccggacga cggcatcggc gagatcttcc tgaagacctc
     6001 gaagcagggc tcgacgctgt cgggcatcac cgatgccttc tccatcgcga tctcgatcgg
     6061 cctgcagtac ggggtgccgc tggagacgta cgtgcagaag ttcatcaaca tgaagttcga
     6121 gccgtccggc atgaccaacg acccggacat ccggttcgcc acgtccatcg tggactacgt
     6181 gttccggcgg ctggcactgg atcacctgtc gaaggagcag cgggagcagc tggggatctg
     6241 ggccatctcc gagcgaaggg agatggcggc ggccgaggcg gccgagaagc tgaacggggg
     6301 tgtcccggct ccctcggctg aggctccgct gggcgagggc ccgggctcct cgccggcccg
     6361 cgccgaggcc gtagctcggc ccgagatcag ggccgccgac gcgccgctct gctaccagtg
     6421 cggctccaag atgcagccgg cgggctcctg ctacgtctgc ccgacctgcg gatccaccag
     6481 cggctgctcg tagtgggacg gagcgcacgc gggccggccc gcgtgcgcgg tgggggaggc
     6541 catggatctg cccttcaagc gcctggatcc ggatgcggag ctgcccgggc cggcgcaccc
     6601 ggacgacgcg gggctcgacc tgcggagcac ggtggacgtg gagatcggcc ccggcgagcg
     6661 ggccctggtc cccaccggcg tggcggtggc gatcccgccc ggtcacgccg ggctggtgct
     6721 gccccgatcg gggctggcat cgaagcaggg cctgaccatg gccaacgccc ccggcctgat
     6781 cgacgccggc taccggggcg aggtgatctg cgcggtggtg aacctggacc gcgggcacgc
     6841 ggtgcggctc gcccgagggg accgcatcgc acagctcgtg gtggtgcccg tgcctcccgc
     6901 gcggccgatg tgggtggagg agctcccgcc ctccgcccgc ggggccgggg gcttcggttc
     6961 gaccgggagc ggctagggtc ccgtgggggc cccgcggccg tgcaggaggt cggggaccgc
     7021 tcgggcgacc ggttgaaggg gcccgcgccg acccctagac tcggggtgtc cctcgaggac
     7081 gcgcgcggac gtggctcagt tggtagagca ccaccttgcc a
//
LOCUS       DASQQQ010000358        23746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_53447, whole genome shotgun sequence.
ACCESSION   DASQQQ010000358 DASQQQ010000000
VERSION     DASQQQ010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 23746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23746
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53447"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..229)
                     /locus_tag="VE646_13295"
     CDS             complement(5..229)
                     /locus_tag="VE646_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD80999.1"
                     /translation="MDAPVGFAQSELAEDPYQDHVGVRVGYVDDSGRELHVFSGIPGE
                     FGEGLPQVDQLSASGSPAALLGRGKVRVLS"
     gene            complement(352..852)
                     /locus_tag="VE646_13300"
     CDS             complement(352..852)
                     /locus_tag="VE646_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81000.1"
                     /translation="MKHRMVIGSILCLAVMASVAVFAAAGLSAPGPERSEPLGPEMAP
                     DTGAGELLVGVEGGVYGTREEAEAANRQMSFGDVAGYYVVPVAQFQGFREGIGASGDF
                     AQVSVFRTQEGALQFAEMARAFAYPATILPERVRSLGGLYAGLGQEANQNGSGPLTRP
                     IPESLP"
     gene            complement(1504..1764)
                     /locus_tag="VE646_13305"
     CDS             complement(1504..1764)
                     /locus_tag="VE646_13305"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HZD81001.1"
                     /translation="MYGHIQNDHLGSYTKSGIWGYTCRYIKGTTKWSTHAFGMAVDIS
                     ASFEPQGSCSSTFNKNFAYVFKHHGWRWGLDFCDPNKPVPHN"
     gene            complement(2204..4906)
                     /gene="ppdK"
                     /locus_tag="VE646_13310"
     CDS             complement(2204..4906)
                     /gene="ppdK"
                     /locus_tag="VE646_13310"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HZD81002.1"
                     /translation="MTKFVYDFSEGNKDMKDLLGGKGANLAEMSNMGLPVPPGFTITT
                     EACLTYLEQDRFPDGLEQQVEEHLRGLERAMGKRLGEVEDPLLVSVRSGAKFSMPGMM
                     DTVLNLGLNEDSLQGLAKQSDGDWRFAYDAYRRFIQMFGKIVMGVPGDAFEEALDAAK
                     DRKGPEAKDTDLEPEDLKRLIGEFLAIYREHTGQHFPTEPRQQMKLAIEAVFKSWNSK
                     RARDYRRQNKISDDLGTAVNIVAMVFGNRGIDSGTGVAFTRDPSTGERIPYGDYLPNA
                     QGEDVVAGIRNTLHLAELKDLDPTSYEGLVQVMDTLERHYRDMCDIEFTIEKGKLWIL
                     QTRVGKRTAFAEWVMAYDMLEEGLIDADTALLRVNANRLEELFKRVISTREGAQPIAQ
                     GLNASPGAATGKVVFSADEAQRWAQGGEKVILVRRETTPDDYHGMVASQGILTSAGGT
                     NSHAAVVARGEGIPAVCGADQIKIDLSSRAFSANGTIVKEGEVVTIDGFTGNVFVGEL
                     SLEESVLERARSGDRAARGEKIWRAFERLMRHADERRGLRVRANADTPEQSQNARERG
                     AEGIGLCRTEHMFLGSERVAAVQQMIFAETQEEEHAAYEQLLPLQREDFVGIFRAMDG
                     LPATVRLLDPPLHEFLPDLTTLSVEVAQSEVRDESGPGLEERKRTLRKVRELHEANPM
                     LGLRGVRLGIVKPGLYAMQVRAIIEAACRVKKEGGEPKVEIMIPLVATRPELEQMRQE
                     LEPAAREVLDREGVELEHLEWGTMIEVPRAAVTAGEIAEVADFFSFGTNDLTQMGFGF
                     SRDDIGKFVGMYEERKLVPANPFVTLDRAGVGRLMRMATDEGRSANPRLHIGICGEHG
                     GDPASVAFCHEIGLDYVSCSPFRVETARLAAGQAVVGQARSAST"
     gene            complement(5049..5843)
                     /locus_tag="VE646_13315"
     CDS             complement(5049..5843)
                     /locus_tag="VE646_13315"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZD81003.1"
                     /translation="MSEPPVPTSDEVRETWDANAAYWDRRMAEPDSWQQTIVFPAVER
                     LLQLRAGERVLEIACGNGLLAQRMAEAGATVTAVDLSEEQLRFARRRVTDPAVELRRV
                     DVGDATALQALTGAPFDAAVCSMGLMDLRDIAPLAEVLPELLRRGGRFVFSVTHPAFN
                     SLSMVMVSERHQESNRFVTEEAVKISRYAQPQVGRGFGIEGQPEPQWYFDRSLSDLVR
                     PFLGEGLVLDALEEPVFGSEVAERGEVRALWTRIPPILAARLRRTV"
     gene            complement(5840..7174)
                     /locus_tag="VE646_13320"
     CDS             complement(5840..7174)
                     /locus_tag="VE646_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81004.1"
                     /translation="MSGRRRSAAWFLAAALLLAACTGSSGQARTPSVSPARPTLLPSS
                     PESPSPAPDGPGSAQAALAKLCPGPAQPPPTGVTPEGPTPPAIRTVERQIQQVRELRF
                     IDPLQVEAVSHPELVKELRASLASSYPRDQYRRRSLAWQTIGAIPPGTDLRAALERFA
                     GTQVIGFYDTLTKQLVFQGGEDPTPFERVTLAHELTHALDDQHYGLSRIDRLGQMCND
                     EAYSAAVGAVEGSATYFMLAYAQRYLSLKDQLQIGLQSGPSTAGIPPFVLQSESWSYT
                     AGLAFMRAMAARGGLQAIDGALQHFPVSTEQVIHPERYPNDVPTPVDVPQLAPELGPG
                     WKDLDVMEVGEEYLSILLGLRIDQAVAIQAAAGWDGGIYRAWSKGDRVAMELATVWDT
                     ATDARGFSDAMESWIGSGGSSAATVLPVDGTHVRVLFASDAPTLELLRKAAG"
     gene            complement(7171..7371)
                     /locus_tag="VE646_13325"
     CDS             complement(7171..7371)
                     /locus_tag="VE646_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81005.1"
                     /translation="MNDAAPARRGRLELYAVAFVLMLAAAITLAIASVGSLNSIRLLW
                     FSAVLSALAIVVAVASVVARGR"
     gene            complement(7368..8729)
                     /locus_tag="VE646_13330"
     CDS             complement(7368..8729)
                     /locus_tag="VE646_13330"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HZD81006.1"
                     /translation="MEAITSLAKRRGIVFPSSEIYGGLRSFWDYGPLGVELKRNVKDA
                     WWRSMVQLRDDVVGLDAAVIMSPRVWKASGHLDSFTDPLVECLSCHQRFRADHLPGWH
                     APQSGHEEDETTLDMEHGPKCPNCGHREFTEPRNFNLMFKTFIGPVEDEADAVYLRPE
                     TAQGMFVDFVTIQQASRRKLPFGIAQQGKSFRNEITPGNFIYRLREFEQMEMEFFVVP
                     GTDEEWHEYWIQERFQWYADLGIRKEHLRLRDHEREELSHYSKRTVDVEYRFPFTDWG
                     ELEGIANRTDFDLKAHAAASGEELSYFDQEKEERYVPYVIEPAAGVDRMVLAFLLDAY
                     RVEEAPTAKGGTEKRTFLALHKDLAPTKVAVLPLSRNEALNPEGRAVFDLVKQHWMCD
                     FDDAGSIGRRYRRQDEVGTPFCVTVDFDTLEDRQVTVRERDSMRQDRLPIEGLVGHLR
                     ERL"
     gene            8943..9386
                     /locus_tag="VE646_13335"
     CDS             8943..9386
                     /locus_tag="VE646_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81007.1"
                     /translation="METCWNCGAKLSPDVEWCGQCYTVIRLQGPLGRERDPSRRLVRL
                     PDTQAKATVARSTATSVGIVGKLIYTALIVGAGVGIYLGLDTWVISAGRAAFGLLVAV
                     VGAYTVLGLVLLRYTWRPSRRLVHVGGEVLSVGRGTGQRGITTRR"
     gene            complement(9394..10143)
                     /gene="uppS"
                     /locus_tag="VE646_13340"
     CDS             complement(9394..10143)
                     /gene="uppS"
                     /locus_tag="VE646_13340"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HZD81008.1"
                     /translation="MSYLSDIDRDRVPAHVGIVMDGNGRWAKQRGLPRTEGHAAGEAA
                     MWDTVQGGVEVGLRWLTMFAFSTENWNRPKAEVRYLMGFNRGLLRRRRDELHRLNVRV
                     RFMGRRDWRVPRSVLREMDVSEELTRYNTGMTLTIAFNYGGRVEIADAVKRLLADHDA
                     GRLKGEKITPESISRRLYHPDMPDPDLIIRTSGEERISNFLLWQAAYSELYFTPVFWP
                     DFNREQLFEAIRDFQKRSRRFGAVPDDEPGP"
     gene            complement(10140..10889)
                     /gene="recO"
                     /locus_tag="VE646_13345"
     CDS             complement(10140..10889)
                     /gene="recO"
                     /locus_tag="VE646_13345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZD81009.1"
                     /translation="MPLYKEQGIVLRSVKLGEADKIVTVMTQGSGKVRAVAKGIRKTT
                     SRFGARLEPFTHVSLMVYRGRGSLDTVSQAEIVDPFRGIRDDLDRFASGETMLEAVDK
                     VAEEHERNLRLFMLLLAGMRTLDRRPSDPAAVAESFLLKLLSLSGFHPSLTACAVCSR
                     PGPALFSSGQGGGVCSNCAELDAGPASPPAFALLASLASCDLPAAGDVTAGPVDPLIR
                     REARAMLFGFAEYHLERRMRSLPILARSLTT"
     gene            complement(10985..11848)
                     /locus_tag="VE646_13350"
     CDS             complement(10985..11848)
                     /locus_tag="VE646_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZD81010.1"
                     /translation="MSAEARVPVRRTAKLYIGGAFPRSESGRSYEVFSHDGVLLAWAT
                     RASRKDLRDAVRAARAAQPAWGGKTAYNRGQVLYRVAELMEGRRAQFERELADAGAAD
                     PVRGVNAAIDRWVWYAGWSDKVHQILGGTNPVAGPYFNFTIPEPTGVVGIVAPQDQSL
                     LGLVSRLAPAIVSGNAAVVLASEHAPLPAVSLSEVLATSDVPGGVVNVLTGFARELVP
                     WLAAHMDVNAIDVTGVPEDMVAEVEQVSAENVKRVVRGPDVDPFSETAQSPYQITALE
                     EYKTVWHPMGA"
     gene            complement(11845..13347)
                     /locus_tag="VE646_13355"
     CDS             complement(11845..13347)
                     /locus_tag="VE646_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZD81011.1"
                     /translation="MSEGIVEEGEDHSSERPVPWRYATSRESTEIVRLQERYDLFVGG
                     RFLEPKSGRYFATINPATEEPLAEVAEAGEEDVDLAARTAREAWEERWRDLPGKERAK
                     YLYRVARALQERSREFSVLETMNNGKPIKESRDVDVPLAAAHFFYYAGWADKLEYAFP
                     GRTPRPLGVAAQITPWNFPLLMCAWKLAPALATGNTAILKPASTTPLTALLLAEVFQD
                     AELPPGVVNVIPGPGSRTGGWLASHPDIAKIAFTGSTEIGKVLRRSVAGTGKRLTLEL
                     GGKAANIVFEDAPLDQAIEGIVNGIFFNQGEVCCAGSRLFVQESVFEPLIDKLKARLD
                     TLRVGDPLDKNTDVGAINSRDQLEKITELVQSGIEEGAEIYQPECPLPERGFWFRPTL
                     FTNVSQSHRIAREEIFGPVLSILTFRTPAEAVEKANNTPYGLSAGIWTDKGSRILWMA
                     ERMKAGVVWSNTFNRFDPASPFGGYKESGFGREGGRHGLEPYLDLDGGAA"
     gene            complement(13358..14347)
                     /gene="deoC"
                     /locus_tag="VE646_13360"
     CDS             complement(13358..14347)
                     /gene="deoC"
                     /locus_tag="VE646_13360"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HZD81012.1"
                     /translation="MAVRAQEELPMSQAAEIDPRGSVLALYTAKEASRLVARVTAVDA
                     VGLEERAAALGKRSIKRESKLWALDLAVRSMDLTTLEGADTAGKIVAMCSKAVRPDPV
                     DPSIPPVAAVCIYPHLIPVAVGQLRGTSVRVASVAGSFPSGLGPLDVKVREVREAVEL
                     GADEIDIVLNRSLFLGGDYVRTFEELVALREAAQDVHLKVILEAGELGSYDRIRQASV
                     LAMAAGADFIKTSTGKIGVAATLPNALCMMEAAREFFLETGRRVGIKVAGGIRASKQA
                     IQYLVLVYETLGAEWMTPERFRIGASSLLNDVLMQIDKERTGRYQGPDYFTID"
     gene            complement(14381..15262)
                     /gene="era"
                     /locus_tag="VE646_13365"
     CDS             complement(14381..15262)
                     /gene="era"
                     /locus_tag="VE646_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HZD81013.1"
                     /translation="MGFRSGIVAVVGRPNVGKSTLVNALVGDKVAIVSDKPQTTRQGI
                     RGIRTTAEAQMVFTDTPGFHKPRTPLGARLNQVVGGSVEGVDAVVLVVDAAAGVGRGD
                     AFVYAHQLRPLSCPKLCAVNKVDAVHGHREVPQLQAASDLGDFEEVVPVSAKTGSGVD
                     LLRELIVEHLPEGPMLYPAEEVTDQPLEARLAEMIREKALEATREEVPHSVAVMVEEL
                     ERNEGITRIHASLIVERDSQKGIVIGRGGAVLKAIGTKARREMEPMLGTKVFLDLRVK
                     VLKEWQRDPKALRRLGL"
     gene            complement(15268..15681)
                     /gene="cdd"
                     /locus_tag="VE646_13370"
     CDS             complement(15268..15681)
                     /gene="cdd"
                     /locus_tag="VE646_13370"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HZD81014.1"
                     /translation="MPSDEVVSDQTVDDLMAAAKGARSNAYAPYSRFAVGAAVLAGGR
                     IFAGVNVENAAYPQGICAERAAVAAAVTAGHREIDAVAVVAGDDAAPPCGGCRQVLHE
                     FGPEMLVVCEASDGGRKRWRLSQLLPEAFGPEDLA"
     gene            complement(15671..16951)
                     /locus_tag="VE646_13375"
     CDS             complement(15671..16951)
                     /locus_tag="VE646_13375"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZD81015.1"
                     /translation="MSGWDWLELVSILILILVVGILGASEVSITRINRIRAYRFLEEK
                     RRGAASLVKIAENPAPFLNVVLLLTLLATIGGTTIATSLAVRLYGGVGEIVATVGMTL
                     LLFVFADVTPKTFVIQRTDRAALLLAPLIAGLGRLVGPVASGLVKVANVIMPGKGLPE
                     GPFITEREILALAEVASEEDQIEEGEKELILSIFEFGDTIVREVMVPRPDIVAIEGDK
                     TLRDVQALVLEHGYSRIPVYQDELDNVLGLVYAKDVLKAIHQGKQDMPLADVVREAHF
                     VPESKKVADLLREMQQEKSHIAMVTDEYGSVSGLVTLEDLLEELVGEIEDEYDRAEPE
                     MVDLGEGRYRVDGRASIDDVNELLDVELPHDGWDSVGGLMLGLMGSIPEEGEEVGFQN
                     ITFKAEKVTRRRVAMVLITRTEEPGSEDAEAHAE"
     gene            complement(16948..17391)
                     /gene="ybeY"
                     /locus_tag="VE646_13380"
     CDS             complement(16948..17391)
                     /gene="ybeY"
                     /locus_tag="VE646_13380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HZD81016.1"
                     /translation="MNDDGDQPRILISDRQSMRIAHDGLIALARRCLVAEGLTRCELS
                     VSFVGREEMSELHATYLDEEVPTDVLSFPLDEQDVDEYGVRILGDVVIAPEVAAANNP
                     ADPAAELRLLLVHGILHLLGYDHEGEQERAAMWARQDRYTRERVR"
     gene            complement(17384..18313)
                     /locus_tag="VE646_13385"
     CDS             complement(17384..18313)
                     /locus_tag="VE646_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007026862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZD81017.1"
                     /translation="MVALLGQRDELLKLVEAAFDSEILVRGNEITISGPQPEATRVGL
                     LFEELITLLERGHELTTSSVGRSIELIKGAEATPTEVLGDVLLTGRGRGIAPKTLGQK
                     RYVDAMRRATVTFAIGPAGTGKTYLAVATAIRSLQQKEVSRIILTRPAVEAGERLGFL
                     PGTLYEKIDPYMKPLYDALFEMTGADSLPRLMERGTVEVAPLAYMRGRTLNDSFIILD
                     EAQNTTPEQMKMFLTRLGFGSKAVVNGDVTQVDLPPGQKSGMVVVEEILQGIDGIDFV
                     RLGARDVVRHKIVQDIVEAYRVYGERRDATADE"
     gene            complement(18469..18831)
                     /locus_tag="VE646_13390"
     CDS             complement(18469..18831)
                     /locus_tag="VE646_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003957742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HZD81018.1"
                     /translation="MSQRPIESSCVFCRIVAGEEPAHVLRVTDTIVAFRDINPQAPTH
                     ILIVPKEHLLSLAEIQDRHGEMLADVAQSAAQLARAENVDGSGWRLVANAGHDAGQSV
                     FHLHFHLLGGRRMHWPPG"
     gene            complement(18853..19947)
                     /gene="dnaJ"
                     /locus_tag="VE646_13395"
     CDS             complement(18853..19947)
                     /gene="dnaJ"
                     /locus_tag="VE646_13395"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZD81019.1"
                     /translation="MATDLYEILGVGRNASQEEIRRAYRRLARELHPDVSGDPGSEER
                     FKEVSAAYEILSDPQKRQQYDLYGQGGGPDMFPFTDVADIFEAFFGTGTFGRRRPQRR
                     SRSQRGEDAGVRVSLTFEEAAFGTERRIDIETLRVCERCGGNGAEPGTSPERCHTCGG
                     AGQVQDVRRSIFGTVMSARPCPTCQGTGEEVASPCTECRSEGRVMRARTVTVEIPAGV
                     SDGVELRVPGEGHAGRAGGSPGDLYVTIGVEPSPLFDRRDQDLFALLDISMEQAALGA
                     ELEIPTLDGAERVKLEPGTDSGSVIRLRGKGIPNLGRRGRGDLYLTVHVETPRDLGKR
                     ERALIQELAELRGEAAGKGASAEARLRRPR"
     gene            complement(19954..21000)
                     /gene="hrcA"
                     /locus_tag="VE646_13400"
     CDS             complement(19954..21000)
                     /gene="hrcA"
                     /locus_tag="VE646_13400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HZD81020.1"
                     /translation="MTDQVELGSRKTAILRAVVEQYVRTGEPVGSETIAEQAGLGVSS
                     ATIRNEMAALEELGYLTHPHTSAGRIPTDHAYRRYVDTLPAGRGLRDPQRRAISGFFA
                     ETMLDLEEVLKGTTQLLSRLTMYAGLAVPPSLSEEALLRVEVVDMGPAVMVLVVGQHG
                     RVEKAIVDRPRDLDGRAVQSLERHLVDEFRGRTLAEAQARALKMAGESAPAERELILV
                     IADTLREMQIGTRSAHVMVGGVANLADEAAHWRRETVRRLFEALEHESELLRLLKEAS
                     AGSEEVSVTIGAEHPTTEGWEASVVAAPFRSGEIPIGTIGVVGPTAMDYVNVVASVRA
                     VARRLSELATELGV"
     gene            complement(21238..22395)
                     /gene="hemW"
                     /locus_tag="VE646_13405"
     CDS             complement(21238..22395)
                     /gene="hemW"
                     /locus_tag="VE646_13405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HZD81021.1"
                     /translation="MSGAVRRPRSPRAGPAAPEPSGIYVHVPFCLTRCGYCDFNAYAG
                     LDHLAPRYVRALLREVELAAPSWSDERIVSVFLGGGTPTTLAVADLKAVLAQLRDRLR
                     VTVDAEVTIEANPDTVDRPKLEGLLAAGFQRLSMGAQSFDRAVLGALERIHDPDSVRR
                     AVRDARAAGYRNVNLDLIYGAHGESLASWERTLWEAVDLEPEHVSAYALTIEPSTPLG
                     RRVQAGAEPAPDPDVQADMFEIACRILADAGYRHYEVSNWAKPGFECVHNLGYWERRP
                     YLGLGAGAHSYRGERRWWNVRPPEEYMLRVGSGRLPMGGEERLDPSDAYLEEVFLRLR
                     ILTGVPSSWLEPDKVRPFLEDGLLFDRGGALVPTERGMLLLNELVLALTGG"
     gene            complement(22392..>23746)
                     /gene="lepA"
                     /locus_tag="VE646_13410"
     CDS             complement(22392..>23746)
                     /gene="lepA"
                     /locus_tag="VE646_13410"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HZD81022.1"
                     /translation="DPADVLRVSAKTGEGVSALLQRVIELIPAPEGRLEDPLRALIFD
                     SSFDPYRGVVASVRVKDGELPSRAKVHYMAADNDDEAEETGVFAPEATPVPSLGPGEV
                     GYLVTGLKDVHQVKVGDTITIAGRDRAHEPLPGYREPLPMVWSGLFPAEGGDYSELRD
                     ALDRLKLNDAALHYEPESSQALGFGFRCGFLGLLHMDIVKERLEREFDLELIATAPNV
                     EFHVIREGEVVVVHNPSEMPVPGTYERVEEPTVLATIITPADHLGAVLELCQSRRGEL
                     VDMKYLSTERIEVHYHLPLAEIIFDFFDQLKSRTRGYASLDYEPWGYEPSELVRVDVL
                     LHGESVDAFSSVVHRDKAYHYGRQMVERLRELIPRQLFDVAIQAAIGSRIVARETVKA
                     KRKDVLAKCYGGDITRKRKLLEQQKKGKARLRRVGKVDVPQEAFIAALRVDQRAGSRP
                     "
BASE COUNT         3576 a         8571 c         7850 g         3749 t
ORIGIN      
        1 cgtctcagct caagacccgc accttccccc gaccgagcag cgccgccggg ctccccgatg
       61 ccgagagctg gtccacctgc ggcagccctt cgccgaactc gccggggatg ccggagaaca
      121 cgtggagctc acggccgcta tcgtccacgt atccgacccg cacgcccacg tggtcctggt
      181 acgggtcctc ggcgagttcg ctctgcgcga accccaccgg cgcgtccatc acgacctggc
      241 aggtcggggt gggggaagga gcgagcgtgg aacctcgccc cttcccgccg cacgacgcga
      301 ccagcgtgca gagggctaac atcgacagac cgaccaccaa ggaggcgcgc ctcacgggag
      361 cgactccggg atcggacggg tgagtgggcc cgagccgttc tggttcgcct cctgtcccag
      421 gcccgcatag aggccgccga ggctccgcac acgctcgggc aggatggtgg ccggataggc
      481 gaacgcccgt gccatctccg cgaactggag cgccccctcc tgggtgcgga acaccgagac
      541 ttgtgcgaag tcccccgagg ctccgatccc ctcacggaag ccctggaact gagccacggg
      601 caccacgtag tacccggcca catcgccgaa gctcatctgc cggttggcgg cttcggcctc
      661 ctcgcgcgtg ccgtacacgc caccctccac gccgaccagc agctcgccgg cacccgtgtc
      721 gggcgccatc tcgggcccga ggggttccga gcgctccggt cccggcgccg agagccccgc
      781 ggccgcgaac accgcgacgc tcgccatcac agccaaacac aggatggatc cgatcaccat
      841 ccgatgcttc atcgcccctc ccctcagtag ccgaacgcgt actggaagtg catgcggtcc
      901 ccctcattgc ctaccgcttc ttcagcccga gtacgaacgg tccgagtatt ccgtgagaac
      961 gacaacgggc gggccggtgc ctgtaaatgc tcagccgcgc cgctccggtg tggaaggcga
     1021 gaaggtgaac gatcagggcc tcaagaactc tgacgaggta cgcaagctcc ggccccccgc
     1081 tgctcctcgc attctgggag gcgccgcctt gggatggggc gcgttcctag gccttggaat
     1141 gattacgggg atcgtggcta cgcagtccat gttcggtgac gtcgatacgg tcggcacact
     1201 tgccgattgg gcagccgcaa gcactctggt tgttgtcggt atcggccttc ttcggggcag
     1261 atggtgggcg ggcgttggcg cgtgggcgac gagcctggga ctcacggtgt ttggcgtcta
     1321 ctgggccatc tggggaaact ctgacaccgg acctggggca cctcttgcaa accccctaac
     1381 gggggcagaa ttgccgtggc cggtcttcgt cgtgccaggt cttgtcatcg cggcgatgct
     1441 gctgccgaga tcgtcgcgca cgtggattca acaggcgaga cgagagcacc gagcgtccag
     1501 ccattagttg tggggaactg gcttattggg gtcgcagaag tccaggcccc agcgccagcc
     1561 gtggtgcttg aacacgtacg cgaagttctt gttgaaggtg ctggaacagc tcccctgcgg
     1621 ctcgaaggag gccgagatgt ccaccgccat cccgaacgcg tgggtgctcc acttggtcgt
     1681 gcccttgatg taccggcagg tgtagcccca gatgccgctc ttcgtgtagg agcccagatg
     1741 gtcgttctgg atgtgcccgt acacgtcgtt atcgaggttg gtcgagaccc ccccttgtcc
     1801 gagaccatct cggtggggta gcccccgagc ttcttgtgga accggaacgt gtacgtgacc
     1861 ccatcgttcg ccgccgtcca cgacatcgag ctggcgtgcg ccttcgcgct gcagggctca
     1921 ccgaaccggt tgacgatctg cgtgtacccg gtgggggtgg ccggatagtg gtaatggtgg
     1981 tcccgccagc cggccctcgc ccagtgccag cccaccaaca gcgggacgca cgccagcgcc
     2041 ccgatgatcc cgagggggag gaggatccgc cgcttggagc ggcgcgagcc agagcccatg
     2101 aatgggccca tgatccacct ccttgttccg cgggggaggc gggagagggg gcgagcctag
     2161 cgccggcccg cccccggttc cgtgatgcgg gtcacatcct gagctacgtg gaggcggacc
     2221 gcgcctgtcc cacgactgcc tggccggccg ccagccgggc cgtctccacc cggaaaggcg
     2281 agcaggagac gtagtccagg ccgatctcgt ggcagaaggc caccgaggcc gggtctcccc
     2341 cgtgctcgcc gcagatcccg atgtggagcc gggggttcgc agatcgtccc tcgtcggtgg
     2401 ccatccgcat caagcgcccc acgccggccc ggtccagcgt cacgaacggg ttcgccggga
     2461 ccagcttgcg ctcctcgtac atgcccacga acttgccgat gtcgtcgcgc gagaagccga
     2521 atcccatctg cgtcaggtcg ttcgtcccga acgagaagaa atcggccacc tcggcgatct
     2581 cgcccgccgt gaccgcggcc ctcgggacct cgatcatggt gccccattcc aggtgctcga
     2641 gctcgacgcc ttcgcggtcc agcacctcgc gcgccgccgg ctcgagctcc tgccgcatct
     2701 gctcgagctc gggtcgcgtg gcgaccaggg ggatcatgat ctcgaccttg ggctcgcccc
     2761 cctccttctt cacccggcag gccgcctcga tgatcgctcg cacctgcatc gcgtacaggc
     2821 ccggcttcac gatccccagg cggacgccgc ggagccccag catggggttg gcctcgtgca
     2881 gctcccggac cttccggagc gtccgcttgc gctcctcgag cccagggccg gactcgtccc
     2941 gtacctccga ctgggccacc tccaccgaca gcgtcgtgag gtcgggcagg aactcgtgga
     3001 gcggagggtc cagcagccgc accgtcgccg ggaggccgtc catggcccgg aagatcccca
     3061 cgaagtcctc gcgctgcagc ggcagcagct gctcgtaggc ggcgtgctcc tcctcctggg
     3121 tctccgcgaa gatcatctgc tggaccgccg ccacccgctc cgagcccagg aacatgtgct
     3181 cggtccggca gagcccgatg ccttccgccc ctcgctcccg ggcgttctgc gactgctcgg
     3241 gcgtgtccgc gttggcccgt acccgcagcc cgcgacgctc atccgcatga cgcatcagcc
     3301 gttcgaacgc cctccagatc ttctccccgc gggcggcccg gtcgccggag cgggcgcgct
     3361 ccaacaccga ctcctccagg gacagctctc ccacgaacac gttgcccgtg aagccgtcga
     3421 tcgtgacgac ctcgccctcc ttcacgatgg tgccgttggc cgagaacgca cgtgaggaca
     3481 ggtcgatctt gatctggtcc gccccacaca cggcagggat gccctcgccg cgagccacca
     3541 ccgcggcgtg cgagttcgtc ccaccggcgc tcgtgaggat gccctgggaa gcgaccatcc
     3601 cgtggtagtc gtccggtgtc gtctcgcggc ggaccaggat caccttctcg ccgccctgtg
     3661 cccaccgttg cgcctcgtcc gcgctgaaca ccaccttgcc ggtggcggcg ccgggggagg
     3721 cgttcagccc ctgggcgatg ggctgggcgc cctcacgggt cgagatcacc cgcttgaaca
     3781 gctcctcgag gcggttggcg ttcacgcgca gcaaagccgt gtcggcatcg atcaacccct
     3841 cctcgagcat gtcgtaggcc atcacccact cggcgaaggc ggtccgcttg cccacccggg
     3901 tctgcaggat ccagagcttg cccttctcga tcgtgaactc gatgtcgcac atgtctcggt
     3961 agtggcgctc gagcgtgtcc atcacctgga ccaggccctc gtacgaggtc ggatccaagt
     4021 ccttcagttc ggccaggtgc agcgtgttgc ggatcccggc gaccacgtcc tcgccctggg
     4081 cgttcggcag gtagtcgccg taggggatcc tctcgcccgt ggagggatcc cgcgtgaagg
     4141 ccacgccggt gccggagtcg atcccgcggt tgccgaacac catggccacg atgttcaccg
     4201 cggtgccgag gtcgtcgctg atcttgttct gacggcggta gtcacgggcg cgcttgctgt
     4261 tccacgactt gaagaccgcc tcgatggcca gcttcatctg ctgtctgggc tcggtgggga
     4321 agtgctgtcc cgtgtgctcg cggtagatgg ccaggaactc cccgatcaac cgcttcagat
     4381 cctccggctc cagatctgtg tccttggcct cggggccctt gcggtccttg gcggcgtcca
     4441 gggcctcctc gaacgcgtcc ccgggcacgc ccatgacgat cttcccgaac atctggatga
     4501 accgccgata ggcgtcgtag gcgaaccgcc agtcgccgtc cgactgcttg gccaggccct
     4561 gcagggagtc ctcgttcagc ccgaggttca ggaccgtgtc catcatgccg ggcatcgaga
     4621 acttcgcgcc ggatcggacg gaaaccagga gcgggtcctc gacctcgccc aggcgcttgc
     4681 ccatggctcg ctccagtccc cggaggtgct cctcgacctg ctgctcgagg ccgtccggga
     4741 agcggtcctg ttccaggtag gtcaggcacg cctcggtggt gatggtgaac ccggggggca
     4801 ccggcagacc catgttcgac atctcggcca ggttggcgcc cttgccgccg agcaggtcct
     4861 tcatgtcctt gttgccctcg gagaagtcat agacgaactt ggtcatcggt gcctccagcg
     4921 cgcgaagggt tggaacatca agtctacgca ggtcggctcc aacgggcctc cacgccgtgc
     4981 tcggcgagaa caggaagagc cgatcaccgg cccatgcagg gccgcgccgc ctcgacgtct
     5041 gcgatcgctt acacggttct ccgcaagcgg gcggcgagga tcggcgggat gcgggtccac
     5101 agcgctcgga cttctccccg ctcggcgacc tcggatccga agaccggctc ctcgagagcg
     5161 tcgaggacca atccctcccc caggaacggt cgaacgaggt cggagagtga gcgatcgaag
     5221 taccactgcg gctcgggttg cccctcgatc ccgaacccgc ggccgacctg cggttgtgca
     5281 taccgagaga tcttcacggc ctcctcggtg acgaaccggt tcgactcctg atgccgttcc
     5341 gagaccatca ccatcgacag gctgttgaac gccgggtgcg tgacggagaa cacgaagcgc
     5401 cctccccgac gcaggagctc cggcagcacc tcggcgagcg gcgcgatgtc gcgcaggtcc
     5461 atcaggccca tgctgcacac cgcggcgtcg aagggcgcac cggtcagggc ctgcagcgcg
     5521 gtcgcatccc cgacatccac ccgtcggagc tctaccgccg gatcggtgac gcgccggcgg
     5581 gcgaaccgga gctgctcctc gctcaggtcg acggccgtca ccgtggcacc ggcctcggcc
     5641 atgcgctgag cgagcagccc gttcccgcag gcgatctcga gcacccgctc accggcccgc
     5701 agctggagga gtctctccac cgcggggaac acgatcgtct gctgccagct gtccggctcc
     5761 gccatccgcc ggtcccagta cgcggcgttg gcgtcccagg tctcccgcac ctcatcggat
     5821 gtcggaaccg gtggttcgct caccccgccg ccttccggag cagttccagg gtgggggcat
     5881 cgctcgcgaa cagcacgcgg acgtgggtcc cgtcgaccgg gagcaccgtg gccgccgagg
     5941 aacccccgct gccgatccac gactccatcg cgtcggagaa cccccgggca tccgtggccg
     6001 tgtcccagac ggtcgcgagc tccatcgcca cccggtcgcc cttggaccag gcccggtaga
     6061 tcccgccgtc ccaaccggcc gccgcctgga tcgcgaccgc ctggtcgatc cgcaggccga
     6121 gcagaatcga caggtactcc tcccccacct ccatcacgtc caggtccttc caccccggtc
     6181 ccagctccgg ggccagttgc ggcacgtcca cgggggtggg cacgtcgttc gggtagcgct
     6241 cgggatggat cacctgctcc gtggagaccg ggaagtgctg cagcgccccg tcgatcgcct
     6301 gcagcccacc ccgggccgcc atggcccgca tgaaggccaa ccccgccgtg taggaccagc
     6361 tctcgctctg caggacgaag ggcgggatcc cggcggtcga gggcccgctc tggagcccga
     6421 tctggagctg gtccttcagg gacaggtagc gctgggcgta ggcgagcatg aaataggtgg
     6481 cgctgccctc caccgcgccg accgcggcgc tgtaggcctc gtcgttgcac atctgcccca
     6541 gccggtcgat ccgcgacagc ccgtagtgct ggtcgtcgag cgcgtgcgtg agctcgtggg
     6601 ccaacgtgac ccgctcgaag ggcgtgggat cctccccgcc ctggaagacc agctgcttgg
     6661 tgagcgtgtc gtagaacccg atcacctggg tcccggcgaa ccgctccagg gccgcgcgga
     6721 ggtccgtgcc gggcgggatc gcgccgatcg tctgccaggc caggctccgc cgtcggtact
     6781 gatcgcgcgg gtaggaggac gcgagcgagg ccctcagctc cttcaccagc tctggatggc
     6841 tgaccgcctc cacctgcagc ggatcgatga accggagctc gcggacctgc tggatctgtc
     6901 gctcgaccgt ccggatagcc ggaggcgtgg gtccctccgg cgtcaccccg gtgggcgggg
     6961 gctgggccgg acccgggcac agcttcgcca gagcggcctg ggccgacccc ggaccgtcgg
     7021 gtgccggaga cgggctctcc ggggacgacg gcagcagcgt gggcctcgcc gggctcactg
     7081 acggtgtccg agcctgaccg ctcgagcccg tgcaggccgc gagcaggagc gcggccgcca
     7141 ggaaccacgc ggcggaccgg cgccggccgc tcatcggcct cgggccacca cgctggccac
     7201 ggcgaccacg atcgcgagtg cggacaggac ggcggagaac cacaggagcc ggatcgagtt
     7261 caaggagccg accgaggcga tcgccagcgt gatggccgcc gcaagcatca acacgaaggc
     7321 caccgcgtac agctccaggc gcccccggcg ggccggagcg gcgtcgttca caggcgctcc
     7381 ctcaggtggc cgacgaggcc ctcgatcggg agccgatcct ggcgcatcga gtctcgctcg
     7441 cgcaccgtca cctgtcggtc ctcgagcgtg tcgaagtcca ccgtcacgca gaacggcgtg
     7501 ccgacctcgt cctgacgacg gtaccggcgt ccgatcgatc cggcgtcgtc gaagtcgcac
     7561 atccagtgct gcttgacgag gtcgaacacg gcccggccct cggggttcag ggcctcgttc
     7621 cgggacagcg gcagcaccgc caccttggtg ggagccaggt ccttgtgcag ggccaggaag
     7681 gtgcgcttct cggtgccgcc cttggcggtg ggggcctcct ccacccggta cgcgtccagc
     7741 aggaaggcca ggaccatgcg gtccacaccg gcggccggct cgatcacgta cgggacgtac
     7801 cgctcctcct tctcctggtc gaagtagctg agctcctcgc cgctggccgc cgcgtgcgcc
     7861 ttcaggtcga agtccgtccg gttggcgatg ccctccagct cgccccagtc cgtgaacggg
     7921 aagcggtact cgacgtcgac cgtgcgcttg gaatagtggg agagctcctc ccgttcgtga
     7981 tctcgcaagc gcaggtgctc cttgcggatc ccgaggtccg cgtaccactg gaaccgctcc
     8041 tggatccagt actcatgcca ctcctcgtcg gtcccgggga ccacgaagaa ctccatctcc
     8101 atctgctcga actcgcgcag ccggtagatg aagttcccgg gcgtgatctc gttgcggaac
     8161 gacttgccct gctgggcgat cccgaagggc agcttcctgc gactggcctg ctggatcgtc
     8221 acgaagtcca cgaacatccc ctgcgccgtc tccggacgca ggtacacagc gtcggcctcg
     8281 tcttccacgg ggccgatgaa cgtcttgaac atcaggttga agttgcgcgg ctccgtgaac
     8341 tcccggtggc cgcagttcgg acacttcggg ccgtgctcca tgtccagggt ggtctcgtcc
     8401 tcctcgtggc ccgactgcgg ggcgtgccag ccggggaggt ggtcggcgcg gaaccgctgg
     8461 tggcagctca ggcactcgac cagcgggtcc gtgaacgagt cgaggtgccc gctcgccttc
     8521 cagaccctcg gcgacatgat caccgccgca tccaggccca cgacgtcgtc ccgcagctgc
     8581 accatggacc gccaccaggc gtccttcacg ttgcgcttga gctcgacgcc gagaggcccg
     8641 tagtcccaga aggaccgtag gccgccgtag atctcgctgg acgggaacac gatgccccgg
     8701 cgcttggcca gggaggtgat ggcctccatg aggtcggcca cgatgggtct cctttcccgg
     8761 ccgctggcga cggccgctgg cgtgccagga agtgtaccgg tgggccgcgc ccgccgagcc
     8821 cgcgcgtccg ctctgtgagg gcagggccga acggccgatc ggctttccgc cttccggccg
     8881 ggttccaagg accgattggg atcgcccgca cgcgtggagt acgatccgcc cgggggaggc
     8941 ccatggagac ctgctggaac tgcggtgcca agctctcgcc cgacgtggag tggtgcgggc
     9001 agtgctacac ggtgatcagg ctccagggcc cgctcggccg cgagcgtgac ccttccaggc
     9061 gcctggtgcg cctccccgac acccaggcca aggcgaccgt ggcgcggagc actgccacca
     9121 gcgtggggat cgtcggcaag ctgatctaca cggcgctgat cgttggggcg ggggtcggca
     9181 tctacctggg cctcgacacc tgggtgatca gcgccggccg agccgcgttc ggcctgttgg
     9241 tcgcggtggt gggcgcctat acggtcctgg gcctggtcct gctccggtac acgtggcgcc
     9301 cctcgcgtcg cctggtacac gtgggcggag aggtcctctc ggtggggcgg ggtacgggcc
     9361 agcgaggcat caccacccgc cggtaggtga cgttcagggg cccggctcgt cgtcggggac
     9421 ggcgccgaag cggcgagagc gcttctggaa gtcgcggatc gcctcgaaga gctgctcgcg
     9481 gttgaagtcg ggccagaaga cgggggtgaa gtagagctcg gaataggcgg cctgccagag
     9541 caggaagttc gagatccgct cctcgccgct ggtgcggatg atcaggtccg ggtcgggcat
     9601 gtcggggtga tacaggcgcc gggagatgct ctccggggtg atcttctccc ccttcagccg
     9661 gccggcgtcg tggtcggcca ggagccgctt cacggcgtcg gcgatctcca cgcggccccc
     9721 gtagttgaag gcgatcgtga gggtcatccc ggtgttgtat ctggtcagct cctccgagac
     9781 gtccatctcc cgcaggaccg agcgcgggac gcgccagtcc cggcggccca tgaaccgcac
     9841 ccgaacgttc aggcggtgca gctcgtcgcg gcgacgccgc agaagcccgc ggttgaagcc
     9901 catcaggtag cggacctcgg ccttgggtcg gttccagttc tcggtggaga aggcgaacat
     9961 cgtgagccac cgcagcccga cctcgacgcc gccctggacg gtatcccaca tcgccgcctc
    10021 gccggcggcg tggccctcgg tccggggcag gccacgctgc ttggcccacc ggccgttgcc
    10081 gtccatgacg atgcccacgt gggccgggac acggtcccgg tcgatgtcgg acaggtagct
    10141 cacgtggtga ggctccgggc caggatcggg aggctccgca tccgccgctc caggtggtac
    10201 tcggcgaagc cgaacagcat cgcgcgagcc tcacgtcgga tcaggggatc gaccggtccg
    10261 gcggtcacgt cgccggccgc cggcagatca cagctcgcga gcgaggccag caacgcgaag
    10321 gccgggggcg aggcgggccc cgcatcgagc tcggcgcagt tcgagcagac cccaccgccc
    10381 tggccgctcg agaagagcgc cggcccgggg cggctgcaca cggcgcacgc ggtgaggctc
    10441 ggatggaagc ccgataggga cagcagcttc aggaggaagg actcggcgac cgccgcgggg
    10501 tccgacggac gccggtcgag ggtgcgcatc cccgcgagga gcagcatgaa cagccggagg
    10561 ttgcgttcgt gctcctcggc caccttgtcc acggcctcga gcatcgtctc cccggaagcg
    10621 aaccggtcca ggtcgtcccg gatgccccgg aaggggtcca cgatctcggc ctggctcacc
    10681 gtgtcgaggg agccccgccc ccgatagacc atcagggaca catgggtgaa tggctccagt
    10741 ctcgccccga agcgactggt ggtcttgcgg atgcccttgg ccacggcccg gaccttgccg
    10801 gagccctggg tcatcacggt gacgatcttg tcggcctcgc ccagcttcac ggaacgcagg
    10861 acgatgccct gttccttgta gaggggcatc gcaggcatcg taccgtggag cgcggagggg
    10921 gttccggaag gcgtgttgcg atgatgtgga atcgccggga cgggcgcggg ctcgagatgc
    10981 tgcctcaggc ccccatcggg tgccagacgg tcttgtactc ctccagggcc gtgatctggt
    11041 aggggctctg cgccgtctcc gagaagggat cgacgtccgg gccgcgcacc acgcgcttca
    11101 cgttctcggc cgagacctgc tcgacctcgg ccaccatgtc ctcggggacg ccggtgacgt
    11161 cgatcgcgtt cacatccatg tgggcggcca gccacgggac gagctcccgg gcgaacccgg
    11221 tcagcacgtt gaccacgccc ccgggcacat ccgaggtcgc cagcacctcc gacagcgata
    11281 ccgccggcag gggcgcatgt tcgctggcca gcaccaccgc cgcgttcccc gagacgatcg
    11341 ccggggcaag acgcgagacc agcccgagca gcgactggtc ctggggcgcc acgatgccca
    11401 ccacgccggt gggctcgggg atggtgaagt tgaagtacgg cccggccacg gggttcgtcc
    11461 cgccgaggat ctggtgcacc ttgtcggacc agcccgcgta ccacacccac cggtcgatcg
    11521 cggcgttcac cccgcgcacc gggtcggccg cgccggcgtc ggcgagctcg cgctcgaact
    11581 gagcgcgccg gccctccatc agctcggcca cgcggtagag gacctggccg cggttgtaag
    11641 cggtcttccc gccccatgcc ggctgcgccg cgcgcgcagc gcggacggcg tcgcgtaggt
    11701 ccttccggga tgctcgggtg gcccacgcca gcagcacccc gtcgtgcgag aagacttcgt
    11761 atgaccggcc ggactcgctg cgcggaaagg caccgccgat gtagagcttg gccgtccgcc
    11821 ggaccgggac cctggcctcc gcgctcacgc tgcgccccca tccagatcga ggtagggctc
    11881 cagcccgtgg cggccgccct cacggccgaa cccgctctcc ttgtaccccc cgaacggcga
    11941 cgcggggtcg aaccggttga aggtgttcga ccagaccacc ccggccttca tccgctcggc
    12001 catccacagg atccgcgagc ccttgtccgt ccagatcccg gccgagaggc cgtacggcgt
    12061 gttgttcgcc ttctccaccg cctcggcggg cgtgcggaag gtcaggatcg acaggacggg
    12121 gccgaagatc tcctcgcgcg cgatccggtg cgactgcgac acgttcgtga acagcgtagg
    12181 ccggaaccag aacccgcgct cgggcagcgg acactcgggc tggtagatct cggcgccctc
    12241 ctcgatcccg ctctgcacca gctcggtgat cttctccagc tggtcgcgag agttgatcgc
    12301 acccacatcc gtgttcttgt cgagcggatc gcccacccgc agggtgtcca gccgtgcctt
    12361 cagcttgtcg atcagcggct cgaacaccga ctcctggaca aagagccggg agccggcgca
    12421 gcagacctcg ccctggttga agaagatgcc gttcacgatc ccctcgatcg cctggtccag
    12481 gggggcgtcc tcgaacacga tgttcgccgc cttgccgccg agctccaggg tcaggcgctt
    12541 gccggtgccg gcgacgctgc ggcgcaggac cttaccgatc tccgtggagc cggtgaacgc
    12601 gatcttcgcg atgtcggggt gtgacgccaa ccagcccccg gtcctgctcc ccgggccggg
    12661 gatcacgttc acgacgccgg gaggaagctc ggcgtcctgg aagacctcgg ccagcagcag
    12721 cgcggtgagg ggcgtggtgg aggccggctt caggatggcg gtgttgcccg tggccagcgc
    12781 gggcgcgagc ttccacgcgc acatcagcag ggggaagttc catggtgtga tctgcgccgc
    12841 cacgccgagc gggcgtgggg tgcgacccgg gaaggcgtac tcgagcttgt cagcccaccc
    12901 ggcgtagtag aagaagtgcg cggccgcgag cggcacgtcg acgtctcggg actccttgat
    12961 cggcttgccg ttgttcatcg tctccaggac ggagaactcg cgagaacgct cctgcagcgc
    13021 ccgagccacc cggtagaggt acttcgcgcg ttccttgccg ggcaggtccc gccagcgctc
    13081 ctcccacgcc tcgcgcgcgg tgcgggcggc caggtcgacg tcctcctccc cggcctcggc
    13141 cacctcggcc agcggctcct ccgtggccgg gttgatggtg gcgaagtagc ggcccgactt
    13201 gggctccaag aagcgccccc cgacgaacag gtcgtagcgc tcctggagcc gcacgatctc
    13261 ggtgctctcc ctcgaggtcg cgtaccgcca cggcaccggt cgctccgagc tgtggtcttc
    13321 cccttcctcg acgatcccct cgctcatgtc cgtcgcctca gtcgatcgtg aagtagtcgg
    13381 ggccctggta gcggccggtc cgctccttgt cgatctgcat caacacgtcg ttcagcaggg
    13441 aagaggcccc gatccggaac cgctccggcg tcatccactc ggctcccagg gtctcgtaca
    13501 ccagcacgag gtactggatc gcctgcttcg atgcccggat ccctccggcc accttgatgc
    13561 ccacccgccg acccgtctcc aggaagaact cccgtgccgc ctccatcatg cagagcgcgt
    13621 tcggcagggt cgcggccacg ccgatcttgc cggtggaggt cttgatgaag tcggctccgg
    13681 ccgccatggc caggacgctg gcctgccgga tccggtcgta gctgcccaac tcccccgcct
    13741 cgaggatcac cttcaggtgg acgtcctgcg ccgcctcgcg cagcgccacg agctcctcga
    13801 aggtcctcac gtagtcgccg cccaggaaca acgagcggtt cagcacgatg tcgatctcgt
    13861 ccgcgcccag ctccacggcc tcgcggacct ctcgcacctt cacgtccagt gggccgagac
    13921 cgctggggaa agaaccggcg acgctggcca cgcgcacgga cgtccctcgg agctggccaa
    13981 ccgccaccgg gatcaggtgc gggtagatgc agacggcggc caccggtggg atcgacggat
    14041 ccacggggtc tggacgcacg gccttggagc acatcgccac gatcttgccc gcggtgtccg
    14101 cgccttccag cgtggtcagg tccatcgacc ggaccgcgag gtccagcgcc cacagcttcg
    14161 actcccgctt gatcgagcgc ttgccgagcg cggccgcccg ctcctccagg cccaccgcgt
    14221 ccacggccgt gaccctggcc accagccgcg aggcctcctt ggccgtgtac agggcgagca
    14281 cggacccccg gggatcgatc tcggctgcct gggacatggg gagctcctcc tgggcccgga
    14341 ccgccatggt agccgaaacg ttgccgcgga ccggacctcc tcacagaccg agtcgccgca
    14401 acgccttcgg gtcccgctgc cactccttca ggaccttgac ccgaagatcc aggaacacct
    14461 tcgtgccgag catcggctcc atctcgcggc gcgccttcgt gccgatcgcc ttgagcaccg
    14521 cgccaccgcg cccgatcacg atgcccttct gcgagtctcg ctccacgatc aggctggcgt
    14581 ggatgcgcgt tatgccctcg ttccgctcga gctcctccac catgacggcg accgaatggg
    14641 gcacctcctc gcgggtggcc tccagcgcct tctcgcggat catctcggcc aggcgcgcct
    14701 ccaggggctg gtcggtgacc tcctccgccg ggtacagcat cggaccctcg gggaggtgct
    14761 ccacgatcag ctcccgcagc agatcgacgc cgctcccggt cttcgcgctc accgggacga
    14821 cctcctcgaa gtcacccagg tcggatgcgg cctgcagctg cggcacctcc cggtgaccgt
    14881 ggacggcgtc gaccttgttc accgcgcaga gcttggggca cgagagcggc cggagctggt
    14941 gtgcgtagac gaacgcgtca ccgcgcccaa ccccggccgc cgcgtccacc accaggacca
    15001 ccgcgtctac gccctccacc gacccaccca cgacctggtt cagccgggcg ccgagggggg
    15061 tcctgggctt gtggaagccg ggggtgtcgg tgaacaccat ctgagcctcg gccgtggtgc
    15121 ggatcccgcg gatcccctgc cgggtggtct gcggcttgtc ggacacgatg gccaccttgt
    15181 cccccaccag ggcgttcact aaggtggact tgcccacgtt cggccggccc accacggcca
    15241 cgatgccgct acggaacccc accggagcta cgcgagatcc tcgggcccga acgcttcggg
    15301 cagcagctgg gacaggcgcc aacgcttcct gcccccatcg ctcgcctcgc agaccacgag
    15361 catctccggc ccgaactcgt ggaggacctg tcggcacccg ccgcagggag gtgcggcgtc
    15421 atcgccggcc accaccgcca cggcatcgat ctcgcggtgt cccgcggtga ccgccgccgc
    15481 caccgccgcc cgctcggcgc agatgccctg cgggtaggcg gcgttctcca cgttcacgcc
    15541 ggcgaagatc cgccccccgg ccaggaccgc cgctcccacc gcgaaccggc tgtacggagc
    15601 gtaggcgttg cttcgagcgc ccttcgccgc cgccatcagg tcgtccaccg tctggtccga
    15661 gaccacctcg tcactcggca tgggcctccg cgtcctcgga cccgggctcc tcggtgcgcg
    15721 tgatcagcac catcgccacg cggcggcgcg tgaccttctc cgccttgaag gtgatgttct
    15781 ggaagcccac ctcctcgccc tcttcgggga tcgaccccat gaggccgagc atcaggcccc
    15841 ccaccgagtc ccaaccgtcg tggggcagtt ccacgtccag cagctcgtta acgtcgtcga
    15901 tggaagcacg gccgtccacg cggtaccggc cttcgccgag gtccaccatc tcgggctcgg
    15961 cgcggtcgta ctcatcctcg atctctccca ccagctcctc gagcaggtcc tccagcgtca
    16021 cgagcccaga gaccgagccg tactcgtcgg tcaccatcgc gatgtgggac ttctcctgct
    16081 gcatctcgcg gagcagatcg gccaccttct tggactcagg gacgaagtga gcctcgcgca
    16141 ccacatcggc gaggggcatg tcctgcttgc cctggtggat cgccttcagc acgtccttcg
    16201 cgtagaccag gcccagcacg ttgtcgagct cgtcctggta gacggggatc cgcgagtagc
    16261 cgtgctcgag caccagggcc tggacgtcgc gcagtgtctt gtccccctcg atcgccacga
    16321 tgtccggtcg agggaccatc acctcccgca cgatcgtgtc gccgaactcg aagatcgaga
    16381 ggatcagctc cttctcaccc tcctcgatct gatcctcctc ggaggccacc tcggccaggg
    16441 cgaggatctc gcgttcggtg atgaacggac cctcgggcag acccttgccc ggcatgatca
    16501 cgttcgccac cttgaccagg cccgaggcca cgggccccac cagccgcccg agccctgcga
    16561 tcaacggtgc cagcaggaga gcggcgcgat cggtgcgctg gatcacgaag gtcttcgggg
    16621 tcacgtcggc gaacacgaag agcagcagcg tcatgcctac ggtggccacg atctcgccca
    16681 ccccgccgta gaggcgcacg gccagagagg tggcgatcgt ggtgcccccg atcgtcgcca
    16741 ggagggtcag gaggagcacg acgttgagga acggagcggg gttctcggcg atcttgacca
    16801 gggacgccgc cccacggcgc ttctcctcga ggaagcggta cgcgcggatc cggttgatcc
    16861 gggtgatcga gacctcggac gcaccgagga tccccaccac caggatcagg atcaggatcg
    16921 agaccagctc cagccagtcc cagccgctca ccggacacgc tccctggtgt accggtcctg
    16981 acgggcccac atggccgctc gctcctgctc cccctcgtgg tcgtagccca ggaggtgcag
    17041 gatgccgtgc acgagcagga gccgcagctc ggccgccggg tcggcggggt tgttggccgc
    17101 cgcgacctcc ggagcgatca ccacgtcgcc caggatccgc acaccgtact cgtccacatc
    17161 ctgctcatcc agtgggaagg acaggacgtc ggtgggcacc tcctcatcca ggtacgtagc
    17221 gtggagctcg gacatctcct ctcggcccac gaacgacacc gatagctcgc atcgggtgag
    17281 tccctcggcg accaggcatc ggcgcgccag ggcgatcagt ccgtcgtggg cgatccgcat
    17341 ggactgacgg tcggagatca agatccgagg ttggtcaccg tcgtcattca tcggcggtcg
    17401 cgtcgcggcg ttctccgtag acgcggtacg cctcgacgat gtcctgcacg atcttgtggc
    17461 ggaccacgtc acgagccccg agccggacga agtcgatccc gtcgatcccc tggaggatct
    17521 cctccaccac caccatgccg gacttctgcc cgggcggcag atccacctgg gtgacgtcgc
    17581 cgttcacgac ggccttcgag ccgaacccga gccgcgtgag gaacatcttc atctgctccg
    17641 gggtggtgtt ctgggcctcg tccaagatga tgaaggagtc gttcagcgta cgtccgcgca
    17701 tgtaggcgag cggcgccacc tccacggtgc cgcgctccat cagccggggc agggaatcgg
    17761 cgccggtcat ctcgaacagg gcgtcgtaca gaggcttcat gtacgggtcg atcttctcgt
    17821 acagggtgcc gggcaggaat cccagtcgct cccccgcctc gacggccggg cgggtcagga
    17881 tgatccggga aacctccttc tgctgcaggg atcggatcgc ggtggccacc gcgaggtagg
    17941 tcttcccggt gccggccgga ccgatggcga aggtgaccgt ggcccgccgc atcgcgtcga
    18001 cgtaccgctt ctggccgagg gtcttgggag cgatcccccg tccccgtccg gtcagcagga
    18061 cgtcgcccag gacctcggtg ggggtggcct cggctccctt gatgagctcg atcgaccgtc
    18121 cgaccgagga cgtagtgagc tcgtgacccc gctcgagcag cgtgatcagc tcctcgaaga
    18181 gcaggccgac gcgcgtggcc tcgggctggg gaccggagat cgtgatctcg ttgccgcgga
    18241 ccaggatctc ggagtcgaag gcggcctcga cgagcttgag gagctcgtcg cgctggccga
    18301 ggagggcgac catgctctga tcgcccggga cgaggatctt cacttgtgcc gtggtgcttg
    18361 ccaaatggga tcgtcgggga tcgtccccgg ttcctcctgt caagaagggg tcgtcaccgg
    18421 acgaccccag ctgcatctga ttctacctcc acacaacggc ccccgcgact acccgggggg
    18481 ccagtgcatc cgccggcccc ccaggaggtg gaaatggagg tggaacacgg actgcccggc
    18541 gtcgtggccg gcgttcgcca ccagacgcca accggacccg tccacgttct ccgccctggc
    18601 gagctgggcc gccgactggg cgacgtccgc cagcatctcg ccgtgccgat cctggatctc
    18661 ggccagggag agcaggtgct ccttgggcac gatcaagatg tgggtcggag cctgcgggtt
    18721 gatatcccgg aaggcgacga tcgtgtcggt cacccgcagg acgtgagccg gctcctcgcc
    18781 ggccacgatc cggcagaaca cgcacgagct ctcgatgggc ctctggctca cgctctcgac
    18841 gatagcccag cctcatcgcg gtcggcgcag ccgagcctcg gcgctcgcgc ccttgccggc
    18901 cgcctctccc cggagctcgg ccagctcctg gatgagggcc cgctcgcgct tgcccagatc
    18961 ccgcggcgtt tcgacgtgaa cggtcaggta caggtcgccg cgaccccgac gcccgaggtt
    19021 cgggatcccc ttgccccgca gacggatcac ggagcccgag tcggtcccgg gttccagttt
    19081 cacccgttct gctccatcca gtgtggggat ctccagctcg gcgcccaggg ccgcctgctc
    19141 catggagatg tccagcagcg cgaacaggtc ctggtcccgt cggtcgaaca gcgggctggg
    19201 ctccaccccg atcgtcacgt agagatcacc gggggaaccg cccgcccgcc cggcgtgacc
    19261 ctcgccgggg acccgcagct ccaccccgtc cgagacgccg gccgggatct ccaccgtcac
    19321 ggtacgggca cgcatcaccc gcccctcgct ccggcactcg gtgcaggggc tggcgacctc
    19381 ctcgccggtg ccctgacagg tggggcaggg tcgggcgctc atcaccgtgc cgaagatgct
    19441 gcgccggacg tcctgcacct gccccgcccc tccacacgtg tggcaccgtt cgggggaggt
    19501 ccccggttcg gcgccgttgc cgccgcagcg ctcgcacacc cgcagggtct cgatgtcgat
    19561 ccgccgctcg gtgccgaagg cggcctcctc gaaggtgagc gatacgcgga cacccgcatc
    19621 ttccccccgc tggctccggc tccggcgctg ggggcggcgg cgcccgaagg tgccggtgcc
    19681 gaagaaggcc tcgaagatgt cggccacgtc ggtgaagggg aacatgtcgg ggccgccccc
    19741 ctgtccgtac aggtcgtact gctgacgctt ctgtggatcg gagaggatct cgtaggcggc
    19801 gctgacctcc ttgaaccgct cctctgatcc gggatccccc gagacgtccg gatgcagctc
    19861 tcgggccagg cgtcggtagg ctctccggat ctcctcctgc gacgcgttcc gccccacgcc
    19921 gaggatctcg tacagatcgg tcgccatcac gggtcagacg ccgagctcgg tggccagctc
    19981 ggacagccga cgggccacgg ctcgcaccga agccacgacg ttcacgtagt ccatggcggt
    20041 gggccccacc acgccgatcg tcccgatggg gatctctccc gagcggaacg gggccgcaac
    20101 caccgaggcc tcccagccct cggtggtggg gtgctcggcg ccgatcgtca ccgagacctc
    20161 ctccgagccg gccgaggcct ccttcagcag cctgagcagc tccgattcat gctccagggc
    20221 ctcgaacaag cgacgcacgg tctcccgacg ccaatgggcg gcctcgtccg ccaggttggc
    20281 cacgcccccc accatcacgt gggcgctccg ggtgccgatc tgcatctccc gcagggtgtc
    20341 ggcgatcacc aggatcagct cccgctccgc gggggcagac tccccagcca tcttcagggc
    20401 ccgcgcctgc gcctcggcca gggtccggcc gcggaactcg tccaccaggt gccgctccag
    20461 gctctgtacg gccctcccat ccaggtccct cggtcgatcg acgatcgcct tctccacccg
    20521 tccgtgctgc cccaccacca gcaccatcac cgcgggcccc atgtcgacca cctcgacgcg
    20581 gagcagggcc tcctcggaga ggctgggggg gaccgccagc ccggcgtaca tcgtgagacg
    20641 cgacagcagc tgggtggtgc ccttcagcac ctcctcgagg tccagcatgg tctcggcgaa
    20701 gaacccggag atggcccgac gctgagggtc gcgcagaccc cggccggccg gcagcgtatc
    20761 cacgtaccgc cggtaggcat ggtccgtggg gatccggccc gcggacgtgt gcggatgggt
    20821 cagatatccg agctcctcaa gggcggccat ctcgttgcgg atggtggccg aggacacgcc
    20881 gaggcccgcc tgctcggcga tggtctcgct ccccacgggc tcgcccgtgc ggacgtactg
    20941 ctcgaccacc gcccgcagga tcgcggtctt ccgggacccg agctccacct ggtcggtcat
    21001 cgcaccagac agtgtattgg cactcgaccc ccctgagtgc caagggacca agggacatgg
    21061 acgacgcagg atctgcgcaa ggtgcgccac cccctcgtgt cgaccctcgg tagggttccc
    21121 gcatcgacag gggggagggg ctgggatggt cggtgcgcga ggcaccggcc cggacctggg
    21181 agcgctgcga accccgacca cggcgctgaa cggtcttgga gctcccgttg gcctgagtca
    21241 tccgccggtc agcgccagga ccagttcgtt cagcagcagc atgcctcgtt cggtgggcac
    21301 cagcgcacct ccacggtcga aaagaagtcc atcctccagg aagggccgaa ccttgtcggg
    21361 ctccagccac gacgagggga cacccgtcag gatccggagg cggaggaaga cctcctcgag
    21421 gtacgcgtcg gagggatcga gacgctcttc gccgcccatc ggcagccggc cggacccgac
    21481 ccgcagcatg tactcctcgg gcgggcggac gttccaccaa cggcgttccc cacgatagga
    21541 gtgagcgccc gcccccagcc ccaggtaggg tcggcgctcc cagtagccga ggttgtggac
    21601 gcattcgaac cccggcttgg cccagttgga cacctcgtaa tgccggtatc cggcatcggc
    21661 caggatccgg cacgcgatct cgaacatgtc cgcctgcacg tccgggtcgg gggccggctc
    21721 cgccccggcc tggacccggc gcccgagcgg cgtcgacggc tcgatcgtga gggcataggc
    21781 gctcacgtgc tcgggctcca ggtccaccgc ctcccacagg gttcgctccc acgacgccag
    21841 ggattcgccg tgggccccat agatcaggtc caggttcacg ttcctgtagc cggcggcgcg
    21901 cgcatcgcga acggcgcgcc gcaccgagtc cggatcgtgg atccgctcca gagcccccag
    21961 cacggctcgg tcgaacgact gggcacccat cgacaaacgc tggaagccgg ccgcgaggag
    22021 gccctccagc ttcggccggt ccaccgtgtc cggattcgct tcgatcgtga cctccgcatc
    22081 gaccgtcacc cgcagccggt cacggagctg ggccaggacg gccttcaggt cggcgaccgc
    22141 caacgtggtg ggggtgccgc cgcccaggaa gaccgagacg atgcgctcgt cgctccagga
    22201 aggggcggcg agctccacct ctcggagcag cgcgcgcacg tacctcgggg ccaggtgatc
    22261 cagtcccgcg tacgcgttga agtcgcagta cccgcagcgg gtcaggcaga acggaacgtg
    22321 cacgtagatg cccgagggct cgggcgccgc tggaccggcg cgtgggctcc tcggccggcg
    22381 gaccgcgccg ctcacggcct ggaccccgcc cgttggtcca cccggagggc ggcgatgaac
    22441 gcctcctgcg ggacgtcgac cttgcccacc cggcggagcc gggccttgcc cttcttctgc
    22501 tgctcgagca gcttacgctt gcgcgtgatg tcccccccgt agcacttggc cagcacgtcc
    22561 ttgcgcttgg ccttcacggt ttcccgggcc acgatcctcg aaccgatcgc cgcctggatc
    22621 gccacgtcga acagctgccg ggggatcagc tcacgcagcc gctccaccat ctgtcggccg
    22681 tagtggtagg ccttgtcccg gtgcaccacg ctgctgaacg cgtcgaccga ctcgccgtgt
    22741 aggagcacgt ccacccgcac cagctccgac ggctcgtacc cccacggctc gtagtcgagc
    22801 gaggcgtacc cgcgcgtgcg ggacttcagc tggtcgaaga agtcgaagat gatctcggcc
    22861 agcgggaggt ggtagtggac ctcgatgcgt tcggtcgaca ggtacttcat gtcgaccagc
    22921 tcgccgcgcc gtgactgaca gagctcgagc acggcgccca ggtgatcggc cggcgtgatg
    22981 atcgtggcca gcacggtcgg ttcctccacc cgctcgtagg tgccgggcac cggcatctcg
    23041 ctggggttgt ggaccacgac cacctcgccc tcacggatca cgtggaactc cacgttcggg
    23101 gccgtggcga tcagctccag gtcgaactcg cgctccagcc gctccttcac gatgtccatg
    23161 tgcagcagcc ccaggaaccc gcaccggaac ccgaacccca gggcctggga gctctccggc
    23221 tcgtagtgca gggcggcgtc gttgagcttc aggcgatcca gtgcgtcccg cagctcgctg
    23281 tagtcgccgc cctcggcggg gaacagcccg ctccagacca tgggcagcgg ctcgcggtag
    23341 ccgggcagcg gctcgtgggc ccggtcccgt ccggcgatcg tgatcgtgtc tcccaccttg
    23401 acctgatgca cgtccttcag gccggtgacg agatagccga cctcccccgg tccgagggag
    23461 gggaccggcg tggcctcggg cgcgaacacg ccggtctcct cggcctcgtc gtcgttgtcg
    23521 gccgccatgt agtgcacctt ggcccgggag ggcagctctc cgtccttcac ccggacggag
    23581 gccaccaccc ctcggtaggg atcgaagctc gagtcgaaga tcagtgcccg caggggatcc
    23641 tccagacgcc cctccggggc ggggatgagc tcgatgaccc gctgcaacag cgcgctcacc
    23701 ccctctccgg tcttggcgga gacccgcagc acgtccgccg gatccc
//
LOCUS       DASQQQ010000359        13542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_327028, whole genome shotgun sequence.
ACCESSION   DASQQQ010000359 DASQQQ010000000
VERSION     DASQQQ010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13542
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327028"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..362)
                     /locus_tag="VE646_13415"
     CDS             complement(117..362)
                     /locus_tag="VE646_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HZD81023.1"
                     /translation="MADDEDVIVQINQLAHEEHELFERESRGEASSQERARLKEIQVQ
                     LDQCYDLLHQRRARRAAGLDLDVESVRRTGTIESPRA"
     gene            complement(413..1999)
                     /locus_tag="VE646_13420"
     CDS             complement(413..1999)
                     /locus_tag="VE646_13420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZD81024.1"
                     /translation="MALLGFEGDNAREKVIVLLTMCFALAMAMLDNTVVNVALPKISE
                     KLGAGVSGLQWIVDGYTLAFASLMLTGGIAGDRYGRKRTFLLGLSVFTLASLACGLSG
                     STEQLIVFRAVQGIGASLLIPGTLSIITVTFPPHERARAIGLWAGISGLALALGPTVG
                     GLMVEHLGWQSVFFLNVPVGVVAFAIGIRTVRESVSEMERRLDIPGLILGTGALFSAT
                     YGLIEANQLGWIDPQIVGSFVGFALLGTAFLLWELRAPHAMMPLRFFRIPAFSAGNTV
                     AFAISLGMFAAFFFLSLYMQLIHGYTAFGAGVRFLPMTLMIIITAPAAGRFAQQHGSR
                     SPMTYGLLLAGGGLLLLGRSLQVESSYLFMLPIFVIMGHGMGATMAPMTAAVMNAVGP
                     QRAGMGSAMTNSSREVGGVLGIALLGTILTTTLKGALLPVLAGLGLPPARQAAIAAAA
                     AHGTLNPSLLAGLDPGLAGRVVDAFGQAFMSGFHQALTFGGVILLVAAVIANRWIPSG
                     APAREEHLAGGQLEVASVEP"
     gene            complement(2138..2974)
                     /locus_tag="VE646_13425"
     CDS             complement(2138..2974)
                     /locus_tag="VE646_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018499995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD81025.1"
                     /translation="MSVSTRRTEGLGGVGMRASWAIHDGLVLTRRNLFRYVRIPTLLV
                     FSTIQPVMFVLLFRYVFGGAITGLPPGIDYVAYLMPGIFVQTAIFGATQTGIGLAEDI
                     NAGVIDRFKSLPAARSAVLAGRTTADLFRNLFVVLLMTAVGYLVGFRFRAGLVDAVLG
                     IGLAVLVGFAFSWISAAIGLAIKNLESVQAASFVWIFPLTFASSAFVPTQGMPGWLQV
                     FARNNPVTIWANTLRGFTLGSMYISSIGESTFGLIWKSGIWIGMILAFFVPVSIRIYR
                     RT"
     gene            complement(2971..3987)
                     /locus_tag="VE646_13430"
     CDS             complement(2971..3987)
                     /locus_tag="VE646_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003954882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZD81026.1"
                     /translation="MSEPAIWAQGIRKRFGEVQALDGVDLRVEPGTVLGLLGPNGAGK
                     TTMVRILTTLLAPDEGRAVVAGHDVIREAEALRRSIGLAGQAAAIDENLTGLENVEMV
                     GRLYHLPRAEAHRRAGQLLERFELTGAANRPARTYSGGMRRRLDLGASLVGRPEVLFL
                     DEPTTGLDPRSRIGMWDVIRELVHDGTTLLLTTQYLEEADRLTDHIVVIDVGKVIAEG
                     TADELKRRVGGEVLALRVADRSRVAEAAGAVLGLGPGGGQADNNTGEIAVPVGAEGTA
                     ILTEAVRRLDSAGIELADIGLRRPTLDDVFLALTGHAAEEPPAEAEPSRGRRGRRAAR
                     EDAS"
     gene            complement(4048..5355)
                     /locus_tag="VE646_13435"
     CDS             complement(4048..5355)
                     /locus_tag="VE646_13435"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HZD81027.1"
                     /translation="MIGTRAVPPPTGRELRACGLAALAEAGLVYLPVHLVLDQSGSVG
                     MSVNALALPFILVFVATTVAACALRTRAEVPTVAGAAAVVLGLSVGRGDLNREVFLVV
                     VLLLVAARAVMLGFRDWREPISASMGWGAVALMVETVLATGPQRDWRPLLIAIVPLFF
                     AASLASRAAIVWTSDPSGDLSDPTRAGWLRRALALIGALAATMVAAIGLGVRGGLLER
                     IGGMTQTVVGGVAAGLAYLMAQLARPIFWLVDRLHVDPSRARSLLRSFREHVSNLDRS
                     HAAWSGGSPVERILGLLAFALLVLVLIAFVRRLRPAPNSARQPVGQPGTVTSEPIEPQ
                     GTTPAPSPRSRRAPPAEAVRRWYAETLELLRRRELDKEPSLTPAEFAAQVAAALPVCA
                     ASFAELTRAYEDVRYGHRSIPEPRLSEVAAAHRSLARILAREP"
     gene            complement(5352..6389)
                     /locus_tag="VE646_13440"
     CDS             complement(5352..6389)
                     /locus_tag="VE646_13440"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZD81028.1"
                     /translation="MGALAGAASLWARGAWSGVSVAVGFEPARAFLGEEVQLRVEIRN
                     AKRLPLPIVRVVARFPPGLLPEPAPKPTALRGHRRRLSIAGWADVTLRLPVVTTERGE
                     YWLEQLDVELSDPFDLTPVRRRFLPERPLLVMPEPRVAIPLRLRRRLPFGRPAPAARL
                     FEDREHIAGVREYEPGDPMHHVHWRLTGHAGRLVTKRYEPTRSAEVLFAIDVANGEPF
                     WYAVDAEMAEETIGWTSFLARQAMGAGWRTGLVANTHLRRGRGPLRVRASSSPGHEAA
                     LFAALARMPNQPTNDLGPVLREATRHLPGRTTIVVISPRAGASLRYEMEVLRRRGSEV
                     VLLSPPEVGVT"
     gene            complement(6496..7452)
                     /locus_tag="VE646_13445"
     CDS             complement(6496..7452)
                     /locus_tag="VE646_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZD81029.1"
                     /translation="MGDADAARALFGSLGERIGERLFGVQEPLRLILVGILTGSHVLI
                     DDVPGVGKTTLVRMLAGLLGLSFQRIQFTPDLMPTDITGTSVLNLRTQEFEFRPGPIF
                     TQLLLADEINRATPKTQAALLEAMQERQVTADGATHPLPHPFFVLATQNPIELEGTFP
                     LPEAQLDRFLLRVRLGYPAERDEERILMTSPEERSLPDRPLPQIPDLGALSKAVSTVR
                     LEHEVRTYVLGLARATRDHPELAVGASPRAVEQMGDAARALALLDGRGYVLPDDVKRL
                     AAPVFGHRLVPTTDARIRDRSAEEILTEVLRSVPVPTELREG"
     gene            7648..9984
                     /gene="lon"
                     /locus_tag="VE646_13450"
     CDS             7648..9984
                     /gene="lon"
                     /locus_tag="VE646_13450"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018353424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZD81030.1"
                     /translation="MATLDTHVLPLLPLTTGVVLPGMVVTLTIESDEARTALAASESS
                     GGELLLVPRVGSRYARIGTVAKVEDVGRLQSGMEALVIRGLHRAVVGSGVAGTGDATW
                     VQVEPRFDPAEVGERARELARQYRATIEALVESRGMPQVREFLRGIREPGAMADTCGY
                     SPDLSFEQKVEVLETLDVEQRLEKVLTWARDVLAEAQLKDKIRTDVQEGLEKNQREFL
                     LRQQMEAIRKELGEAPEENVVEEYRARIERANMPEDARKEAERELGRLERTSEQSPEY
                     GWIRTYLDWMTELPWDVRTEDNLDIADARRILDEDHTGLSDVKDRIIEYLAVRKLREE
                     RGMGPAAGRGSGAILTLVGPPGVGKTSLGESVARALGRGFVRVSLGGIHDEAEIRGHR
                     RTYVGALPGRIVRGLKDAGTKNPVFMLDEIDKVGSDWRGDPSSALLEVLDPAQNHTFR
                     DHYLEVDLDLSEVLFIATANVSETIPGPLLDRMEVIRLDGYTEDEKVAIARDHLLSRQ
                     VERNGLRADEVEVTDDAVRQVVVDHTREAGVRNLERELGKVLRKVATRLAAGEMQPPV
                     TVGAAQVRGYLGRPRFFFEAAERTSVPGVATGLAVTGTGGDVLFIEATRMDGEPGLTL
                     TGQLGDVMKESAEIALSYVRSHAADLGIAPGGLKGRFHVHVPAGAVPKDGPSAGITMV
                     TALASLLTGRPVKATVGMTGEVTLQGRVLPIGGVKQKVLAAHRAGLQEILLPQRNEGD
                     LDDVPEKVREQIAFHPVATVEEVLALALSEGVEARPAA"
     gene            10031..10879
                     /locus_tag="VE646_13455"
     CDS             10031..10879
                     /locus_tag="VE646_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZD81031.1"
                     /translation="MGASASGTFTIAGELTVNRLGFGAMRITGTGIWGPPKDRDECIR
                     VLRRAVDLGVNLVDTADSYGPDVSEELIAEALHPYPGELVIATKGGFQRPGPDRWEED
                     GRPEHLRAACEGSLRRLRVERIDLYQLHRIDPKVPAEDQLATLRDLHAEGKIRHIGLS
                     EVSVEQLRLAQDAIPVASVQNLYNVRNRRSESVLEVCGAEGIAFLPWYPLNVGKLTRS
                     GSPVEAVASTRGATPAQVALAWLLARSPVMLPIPGTSSVAHLEENVAAAELHLSPDEV
                     ESIGAV"
     gene            10889..11311
                     /locus_tag="VE646_13460"
     CDS             10889..11311
                     /locus_tag="VE646_13460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HZD81032.1"
                     /translation="MIRLDPSDATLAHADERLRTELIAWLTTVGADGTPKSTPVWFLW
                     DGRDLLVYSRPGKPKLRNLASNPHVSVHLEGNGLGGDNVILEGVAHLDDGAPPADRVS
                     EYLRKYRDRIAQGGWTPASFASDYTVPVRIDPTRIRVW"
     gene            11316..12233
                     /locus_tag="VE646_13465"
     CDS             11316..12233
                     /locus_tag="VE646_13465"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZD81033.1"
                     /translation="MRALVASGGGVELRQVPEPETGPDRALVRVRSVSLNRGECAALR
                     TAEDGWRPGWDLAGEVLRAAGDGTGPPEGARVVGWVNGGSWAELAAVRTDHLAPVPDE
                     LEPEVSSTLPVAGLTALGTLALGAPLPGRRVAVTGGAGGVGRFAVQLAHRDGAHVTAV
                     VGRPERAEGLHELGADEIVVGLEPEGPAFDLILESAGGASLAAAVSRVSADGMIVTFG
                     NSSNEPVTFDPRTLYRKGGPRIVGFFVTWLLERGRIGARELGFLARLVALGQLRSEVA
                     LRTDWSDAASAIGALMDRLVDGKAVLSIG"
     gene            complement(12279..12572)
                     /locus_tag="VE646_13470"
     CDS             complement(12279..12572)
                     /locus_tag="VE646_13470"
                     /inference="COORDINATES: protein motif:HMM:NF025601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HZD81034.1"
                     /translation="MEREVRWRSIRVTGEQLVVTVKRLMAGRVVRRIIVKTDEDHTLL
                     EIPIGDEVPKGMLEPLWAGLGAVGAIAPALTLEVEELAPVGDDPLDEHVAHVT"
     gene            complement(12666..>13542)
                     /locus_tag="VE646_13475"
     CDS             complement(12666..>13542)
                     /locus_tag="VE646_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018892351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZD81035.1"
                     /translation="YHGSVDETIIVTTDDGTGSAIRPGNIGPAVDPAMTTRVVEFNDP
                     AALEEALAHEDVACVLAEPALTNIGIVLPQAGYHETLRELTRRYGTYLVIDETHTICA
                     GPGGATKAWGLEPDFLTIGKPLASGVPAACYGMSEEVASRVQWYMDGLPSTDVGGVGG
                     TLSGNALSLAAMRATLENVLTDEAFERMIELGARWAEGVKAGIDAHGLPWEVQRLGCR
                     AEYWFCPEPPTTGGRAAAADDHELARFTHLYALNRGVLLTPFHNMALMSPATTAEDVD
                     RHTAVFDEMCGELTI"
BASE COUNT         2028 a         4840 c         4905 g         1769 t
ORIGIN      
        1 agcgctcgga cccaaccacc ccatcgcaga gcgcgaccac ctcgacgtcg ccgatccgaa
       61 ggctcccgtg cggcaccgac cgcgatcgta gcgccgacac cgctccggga cggtgctcag
      121 gcccgggggc tctcgatcgt gccggtccgg cggacgcttt cgacatccag gtccagaccg
      181 gccgcccgcc gcgcccgccg ctggtgcagc aggtcgtagc actggtccaa ctggacctgg
      241 atctccttca gccgggcccg ctcctgggac gaggcctcgc cccgggactc cctctcgaag
      301 agctcgtgct cctcgtgggc cagctggttg atctgcacga tgacgtcctc gtcgtcggcc
      361 atgcgatgac ctcccgccca accctactac cgggggcccg ggcgggcagc gatcagggct
      421 cgaccgatgc cacctcgagc tgaccgccgg ccaggtgctc ctcgcgggcc ggagcaccgc
      481 tggggatcca ccggttcgcg atcaccgccg cgaccagcag gatcacgccg ccgaacgtga
      541 gcgcctggtg gaacccactc atgaaagcct ggccgaaggc gtccacgacc cgcccggcga
      601 gcccggggtc cagcccggcc agcagcgatg ggttcagcgt gccgtgcgcg gccgcggcgg
      661 cgatggccgc ctgtcgggcc ggcggcaaac cgaggccggc cagcacgggc agcagcgcgc
      721 ccttgagcgt ggtggtcagg atggtcccca gcagggcgat gccgagcacc ccgccgacct
      781 cccgggacga gttcgtcatg gccgagccca tcccggcccg ctgcgggccg accgcgttca
      841 tcaccgcggc cgtcatcggt gccatggtgg cccccatgcc gtggcccatg atcacgaaga
      901 tcggcagcat gaacaggtag gagctctcga cctggaggct gcggccgagc agcagcagcc
      961 ctccgccggc cagcagcagg ccgtacgtca tcggcgagcg cgagccgtgc tgctgggcga
     1021 agcgaccggc ggccggcgcg gtgatgatga tcatcaacgt catgggaagg aaccgcaccc
     1081 ccgccccgaa cgccgtgtag ccgtggatca gctgcatgta caggctgagg aagaagaagg
     1141 cggcgaacat ccccagcgag atggcgaacg ccaccgtgtt gccggctgag aaggcgggga
     1201 tgcggaagaa ccgcagcggc atcatcgcgt gcggggcgcg cagctcccac agcaggaagg
     1261 ccgtccccag cagggcgaag cccacgaacg agcccacgat ctgcgggtcg atccagccca
     1321 gctggttggc ctcgatcagc ccgtaggtgg ccgagaacag cgcgccggtc cccaggatca
     1381 gacccgggat atccagacgc cgttccatct ccgacacgga ctcgcgcacg gtccggatcc
     1441 cgatggcgaa cgccaccacc ccgaccggga cgttcaggaa gaacaccgac tgccacccca
     1501 ggtgctcgac catcagcccg cccaccgtgg gacccagggc cagggccagc ccggagatgc
     1561 cggcccacag gccgatggcc cgggcccgct cgtgcggcgg gaaggtgacc gtgatgatcg
     1621 ataacgtgcc cgggatcagc agcgaggcgc cgatgccctg cacggcccgg aagacgatca
     1681 gctgctcggt gctgccggac aggccgcagg ccagcgaggc cagtgtgaac accgacagcc
     1741 cgagcaggaa ggtgcgcttg cgcccgtaac gatccccggc gatcccgccg gtcagcatca
     1801 gcgaggcgaa cgccagcgtg tacccgtcca cgatccactg caggcccgag acgccggcgc
     1861 cgagtttctc gctgatcttc ggcagggcca cgttcacgac ggtgttgtcg agcatggcca
     1921 tggccagggc gaagcacatc gtgaggagga cgatcacctt ctcgcgggcg ttgtccccct
     1981 cgaatccgag cagcgccaaa ccgaactcct tcccgaacgg gagccagccg gcgggcccgg
     2041 ccagacgatc gacccaggat acgtgtgaag cgtgacaacc cgccggacgg gctcggttgt
     2101 tcccgagggt ccgagctcct cggccggcgg gcgatcctca ggtccgccgg tagatccgga
     2161 tcgacaccgg gacgaagaag gccaggatca tgccgatcca tatccccgac ttccagatca
     2221 gaccgaaggt ggactccccg atcgaggaga tgtacatgct gcccagggtg aagccacgca
     2281 gcgtgttggc ccagatcgtg accgggttgt tgcgggcgaa gacctgcagc cagcccggca
     2341 tgccctgggt gggcacgaaa gccgagctgg cgaaggtgag ggggaagatc cacacgaagc
     2401 tcgccgcctg gaccgactcg aggttcttga tcgccaggcc gatcgcggcc gagatccacg
     2461 agaacgcgaa gcccaccagg acggcgagcc cgatccccag cacggcatcc accaggccgg
     2521 cccggaaccg gaagcccacc aggtagccca ccgccgtcat cagcaggacc acgaacaggt
     2581 tccggaacag gtcggccgtg gtccgcccgg ccagaacggc cgagcgggcg gccggcagcg
     2641 acttgaaccg gtcgatcacg ccggcgttga tgtcctccgc caggccgatc ccggtctgcg
     2701 tggcgccgaa gatcgccgtc tgcacgaaga tccccggcat gaggtacgcc acgtagtcga
     2761 tgcccggcgg gagcccggtg atggcaccgc cgaacacgta ccggaacagc agcacgaaca
     2821 tcaccggctg gatcgtggag aacaccagca gggtggggat ccgcacgtag cggaagaggt
     2881 tccggcgggt caggaccagg ccgtcgtgga tcgcccagct cgcgcgcatc cccacgccac
     2941 ccagcccctc ggtccgacgg gtcgagacgc tcacgatgcg tcttcccgcg ccgcccggcg
     3001 cccgcgtcgc ccgcggctcg gctcggcctc cgcgggcggc tcctcggccg cgtgcccggt
     3061 gagggccagg aacacgtcgt ccagcgtggg tcgtcgcagc ccgatgtccg ccagctcgat
     3121 cccggccgag tccaaccggc ggaccgcctc ggtcaggatg gccgtgccct cggcgcccac
     3181 cggaacggcg atctcgccgg tgttgttgtc ggcctgcccg ccgccggggc ccaggccgag
     3241 caccgcgccc gccgcctcgg ccacccggct ccgatcggcc acccggagcg cgaggacctc
     3301 gccccccacg cggcgcttca gctcgtcggc ggtgccctcc gcgatcacct tgcccacgtc
     3361 gatcaccacg atgtggtcgg tcagccgatc ggcctcctcc aggtactgcg tggtcagcag
     3421 gagcgtggtc ccgtcgtgta cgagctcgcg gatcacgtcc cacatgccga tccggctcct
     3481 cgggtccagg ccggtggtgg gctcgtccag gaacagcacc tcgggccggc ccaccaggct
     3541 cgcccccagg tcgagccggc ggcgcatgcc gcccgagtag gtccgggccg gccggttggc
     3601 ggcaccggtc agctcgaagc gctccaggag ctgcccggct cgtcggtgcg cctcggcccg
     3661 agggaggtgg tacaggcggc ccaccatctc cacgttctcc aggccggtca ggttctcgtc
     3721 gatcgcggcg gcctgcccgg ccaggccgat cgaccgccgg agcgcctcgg cctcccggat
     3781 cacgtcgtgg cccgcgacga cggcccgtcc ctcgtccggg gcgagcaggg tggtgaggat
     3841 ccgcaccatc gtggtcttgc cggccccatt cggccccagc aggccgagca ccgtgcccgg
     3901 ctccacgcgc agatccacgc cgtccagcgc ctggacctcg ccgaaccgct tgcggatgcc
     3961 ctgggcccag atggcaggtt cgctcacacg tcgctcccgg tgggatgtcc gcgcatgcta
     4021 cctgcccggg ccgcaagcga cgacgctcta cggctcgcgc gccaggatcc gggcgagcga
     4081 tcggtgcgcg gcagccacct cggatagccg cggctcgggg atcgagcggt gcccgtaccg
     4141 cacgtcctcg taggcgcggg tgagctcggc gaagctcgcg gcgcagacgg ggagcgccgc
     4201 ggccacctgg gccgcgaact ccgccggcgt gagcgagggt tccttgtcca gttcgcgtcg
     4261 ccgcaggagc tccagcgtct ccgcgtacca gcgacggacc gcttcggcgg gcggcgcccg
     4321 tcgggatcgg ggcgaaggag cgggcgtggt cccctgtggc tcgatgggct cgctcgtcac
     4381 ggtccctggc tgcccgaccg gttgccgggc ggagttcggc gcggggcgga gccggcggac
     4441 gaaagcgatc aggaccagga ccaggagggc gaaggcgagc aggcccagga tccgctcgac
     4501 cggcgagccg ccgctccagg ccgcgtggga tcggtccagg ttggacacgt gctcgcggaa
     4561 cgagcgcagc aggctccggg cccggctggg atccacgtgc agccggtcaa ccagccagaa
     4621 gatcggccgc gccagctgcg ccattaggta ggccagcccg gcggccaccc cgcccacgac
     4681 cgtctgcgtc atgccgccga tccgctccag cagcccgccc cggaccccca gcccgatggc
     4741 ggccaccatc gtcgcggcca gcgcaccgat cagcgccagg gcccggcgga gccaccccgc
     4801 ccgcgtcggg tccgacaggt cccccgacgg atccgaggtc cagacgatcg cggcccggct
     4861 ggccagcgag gccgcgaaga acagcgggac gatggcgatc agcagcggcc gccagtcccg
     4921 ttgcggtccg gtggcgagca cggtctccac catcagggcc acggcgcccc atcccatcga
     4981 agccgagatc ggctcgcgcc agtcccggaa cccgagcatc accgctcggg ccgccaccag
     5041 cagcaggacc accaccagaa agacctcccg gttgaggtcc ccccggccca cggacagccc
     5101 caggaccacg gccgcggctc ccgccaccgt cggcacctcg gcccgcgtcc gcagggcgca
     5161 cgccgcgacg gtggtcgcga cgaagacgag gatgaagggc agggccagcg cgttcacgct
     5221 catccccacg ctcccggact ggtccaggac caggtgcacc ggcaggtaga ccaggccggc
     5281 ctcggccagt gcggccaggc cgcaagcccg gagctcccgg cccgtggggg gcggcaccgc
     5341 ccgcgtgccg atcacgtcac gccgacctcc ggcggcgaca gcagcaccac ctcggaccct
     5401 cggcgtcgca gcacctccat ctcgtacctg agggaggcgc cggcccgagg cgagatcacg
     5461 acgatcgtgg tccgccccgg caggtgccgg gtggcctcgc gcagcaccgg accgagatcg
     5521 ttcgtgggct ggttcggcat gcgcgccagc gcggcgaaca gggccgcctc gtggcccggc
     5581 gacgacgagg cccggacccg cagtggaccc cgccctcggc gcaggtgggt gttggccacg
     5641 agtccggtcc tccagcccgc ccccatggct tgacgggcca ggaagctggt ccagccgatc
     5701 gtctcctcgg ccatctccgc atccaccgcg taccagaagg gttccccgtt ggccacgtcg
     5761 atcgcgaaga gcacctcggc cgaccgggtg ggctcgtacc gcttggtcac cagccgcccg
     5821 gcgtgtccgg tgagcctcca atggacgtga tgcatcgggt cgcccggctc gtattcgcga
     5881 acccccgcga tgtgctcacg gtcctcgaac agccgtgccg ccggtgccgg ccggccgaac
     5941 ggcagccggc gtcggagccg gagcgggatg gccacgcggg gctccggcat cacgaggagc
     6001 ggtcgctcgg ggaggaagcg acggcggacc ggggtcagat cgaaggggtc ggacagctcc
     6061 acgtccagct gctccagcca gtactcgccc cgctcggtgg tgaccaccgg gagccggagg
     6121 gtcacgtccg cccagccggc gatcgacagc cgtcgccgat ggccccggag cgccgtgggc
     6181 ttcggggcgg gctcgggcag caggccgggg gggaagcggg ccaccacccg gacgatcggc
     6241 aggggcagtc gcttcgcgtt gcggatctcg acccgtagct ggacctcttc gcccaagaag
     6301 gcccgcgccg gctcgaaccc gaccgccacg ctcacgccgc tccatgcccc ccgggcccac
     6361 agggatgccg ccccggccag ggcgcccacg gcggccgccc acagcagcag ggtcagctca
     6421 ccccaggcgg tggcgaacag cgcggcaagg atcgcgaccc cgaggacgat ccgccgccgt
     6481 tcccggctgt ccgtgtcagc cctcccggag ctccgtgggg accggaaccg accgcaggac
     6541 ctcggtcagg atctcctcgg cgctgcggtc tcggatccgc gcgtcggtgg tgggaaccag
     6601 gcggtgcccg aagacgggag cggcgaggcg cttcacgtcg tcgggcagga cgtacccgcg
     6661 cccgtccagg agcgccaggg cccgagccgc gtcgcccatc tgctcgacgg cgcgggggct
     6721 ggcgcccacc gcgagctcgg gatggtcccg ggtggcccgg gccagcccca gcacgtaggt
     6781 gcggacctcg tgctccagac ggaccgtgct caccgccttg gacagcgctc cgagatccgg
     6841 gatctgcggc aggggtcggt cgggcaggct ccgctcttcg ggagaggtca tgaggatccg
     6901 ctcctcgtcg cgctcggccg ggtagccgag ccggacccgg agcaggaagc ggtccagctg
     6961 cgcctccggc agggggaacg tgccctcgag ctcgatcggg ttctgggtgg ccaggacgaa
     7021 gaagggatgg ggcaatgggt gcgtggcccc gtccgccgtc acctgccgct cctgcatggc
     7081 ctccagcaga gcggcctggg tcttgggggt ggcccggttg atctcgtcgg ccagcaggag
     7141 ctgggtgaag atcggtccgg gccggaactc gaactcctgg gtcctcaggt tcagcaccga
     7201 ggtcccggtg atgtcggtgg gcatcaggtc gggggtgaac tggatccgct ggaaggacag
     7261 tccgagcagg ccggccagca tgcggaccag cgtggtcttg cccaccccgg gaacgtcgtc
     7321 gatcagcacg tgggagccgg tcaggatgcc caccaggatc agacgcagcg gttcctggac
     7381 cccgaacagg cgctccccga tccgctcgcc gagcgatccg aacagcgccc gcgccgcgtc
     7441 cgcgtcaccc atgccagcaa ggatagatgc ctggtcgcgg ggccggcctg ccgccgggcc
     7501 tcaccggtcc atttcttgta atcccgctta caatcctgga atacccgtcc cgggtgggtc
     7561 gttgttgcag gtgcaaggca tcgagccggg ccgggtgctc ggccgtcgcc ggcacgcgag
     7621 cacacacgag gagaacaagg aagcgacatg gccacactcg acacccacgt cctgcccctg
     7681 ctgccgctca ccaccggcgt ggtcctgccc ggcatggtgg tgaccctcac catagagtcc
     7741 gacgaggccc gcacggccct ggcggcgagc gagtcatcgg gcggcgagct cctgctcgtg
     7801 ccccgggtcg gttcccgcta cgcgcggatc ggtacggtgg ccaaggtcga ggacgtggga
     7861 cggttgcagt ccgggatgga ggcgctggtg atccggggcc tgcaccgcgc cgtggtgggc
     7921 agcggcgtgg ccgggaccgg cgacgccacc tgggtccagg tggaaccgcg gttcgaccca
     7981 gccgaggtcg gcgagcgcgc ccgggagctg gcgcgtcagt accgggccac gatcgaggct
     8041 ctggtggagt cccgcggcat gccccaggtc cgcgagttcc tccgcggcat ccgtgagccc
     8101 ggcgcgatgg cggacacgtg cggttactca cccgacctgt ctttcgagca gaaggtggag
     8161 gtcctggaga cgctcgacgt ggagcagcgg ctggagaagg tcctgacctg ggcccgcgac
     8221 gtcctggccg aggcgcagct gaaggacaag atccgcaccg acgtgcagga gggcctggag
     8281 aagaaccagc gtgagttcct cctgcgccag cagatggagg cgatccgcaa ggagctcggc
     8341 gaggcccccg aggagaacgt ggtcgaggag taccgggcga ggatcgagcg agccaacatg
     8401 cccgaggacg cgcgcaagga ggccgagcgc gagctgggcc ggctggagcg gacctcggag
     8461 cagtcgccgg agtacgggtg gatccgcacc tacctggact ggatgaccga gctgccatgg
     8521 gatgtgcgaa ccgaggacaa cctggacatt gccgatgccc ggcggatcct ggacgaggac
     8581 cacacgggcc tgtccgacgt gaaggaccgc atcatcgagt acctggcggt gcgcaagctg
     8641 cgcgaggagc ggggcatggg cccggccgcg ggccggggct caggcgcgat cctcaccctg
     8701 gtcggtcccc ccggcgtggg caagacctcc ctcggcgagt ccgtggcgcg cgcgctgggg
     8761 cgcgggttcg tccgggtctc gctgggcggg atccacgacg aggccgagat ccgggggcac
     8821 cggcggacct acgtgggcgc gctccccgga cggatcgtgc gagggctgaa ggacgcgggc
     8881 acgaagaacc ccgtgttcat gctcgacgag atcgacaagg tgggctccga ctggcgcggc
     8941 gatccctcca gcgcgctgct cgaggtgctg gacccggccc agaaccacac cttccgggac
     9001 cactacctgg aggtggacct ggacctctcc gaggtcctgt tcatcgccac ggccaacgtg
     9061 tccgagacga tccccggtcc gctgctcgac cggatggagg tcatccgcct ggacggctac
     9121 accgaggatg agaaggtggc gatcgcccgc gatcacctgc tcagccgcca ggtggagcgc
     9181 aacggcctga gggccgacga ggtcgaggtc accgacgacg cggtccgaca ggtggtggtc
     9241 gaccacacgc gggaggccgg ggtacggaac ctggagcgcg agctcggcaa ggtgctccgc
     9301 aaggtcgcga cccggctggc cgcgggcgag atgcagcccc cggtcacggt gggagcggca
     9361 caggtgcgcg ggtacctggg acggcccagg ttcttcttcg aggcggccga gcggaccagc
     9421 gtgcccgggg tggccaccgg tctggcggtg accgggacgg gcggcgacgt gctgttcatc
     9481 gaggccacgc ggatggacgg ggagcccggg ctcaccctca cgggacagct gggtgacgtg
     9541 atgaaggagt ccgccgagat cgccctgtcg tacgtgcgct cccacgcggc ggacctgggg
     9601 atcgcgccgg ggggcctgaa ggggcggttc cacgtccacg ttccggccgg ggcggtgccg
     9661 aaggatgggc cgagcgccgg catcacgatg gtcaccgcgc tggccagcct cctgaccggg
     9721 cgaccggtga aggccaccgt gggcatgacc ggcgaggtca ccctgcaggg acgggtcctg
     9781 ccgatcggcg gcgtgaagca gaaggtgctc gcggcccatc gggcgggcct gcaggagatc
     9841 ctgctgcccc agcgcaacga gggcgacctg gacgacgtgc cggagaaggt ccgtgagcag
     9901 atcgcgttcc acccggtggc cacggtcgag gaggtgctgg cgctggcgct ctccgagggc
     9961 gtggaggcgc gtccggcggc gtgagccggg taggttcgga cctcggacca ccgaccgcga
    10021 aaggagcctg atgggtgcct cggcctcggg gacgttcacc atcgccggtg agctcaccgt
    10081 caaccgcctc ggtttcggag cgatgcggat caccgggacg ggcatctggg gcccgccgaa
    10141 ggaccgtgac gagtgcatcc gggtgctccg ccgggcggtg gacctcggcg tgaacctggt
    10201 cgacaccgcc gactcctatg gcccggacgt gagcgaagag ctgatcgccg aggcgctcca
    10261 tccgtatccc ggggagctgg tgatcgcgac caagggcggg ttccagcgtc ccgggccgga
    10321 ccgctgggag gaggacggcc ggcccgagca cctgcgggcg gcctgcgagg gctcgctgcg
    10381 gcggctgcgg gtggagcgga tcgacctgta ccagctgcac cggatcgacc ccaaggtgcc
    10441 ggccgaggac cagctggcca cgctgcgcga cctccatgcc gagggcaaga tccgtcacat
    10501 cggcctgtcc gaggtgagcg tggagcagct tcggctggcg caggacgcca tcccggtggc
    10561 gagcgtgcag aacctctaca acgtgcggaa ccgccgctcc gagagcgtgc tggaggtctg
    10621 cggagccgaa gggatcgcct tcctgccctg gtacccgctg aacgtgggca agctcacgcg
    10681 ctcggggagc ccggtggagg cggtcgcctc gacgcgcggg gccacgccgg cgcaggtcgc
    10741 gctggcctgg ttgctggctc gctcgccggt gatgctcccc atcccgggca cctccagcgt
    10801 ggcccacctg gaggagaacg tcgcggcggc cgagctccac ctgtcccccg acgaggtcga
    10861 gtcgatcggc gccgtctaga gtggaacggt gatccggctc gacccctccg atgccaccct
    10921 cgcgcacgcg gacgagcgcc tgcggaccga gctgatcgcc tggttgacga cggtcggagc
    10981 cgacggcacg ccgaagtcca cgccggtgtg gttcctgtgg gacggcaggg acctcctggt
    11041 ctactcgcgg cccgggaagc ccaagctccg gaacctggcc agtaacccac acgtgtcggt
    11101 gcacctggag ggcaacgggc tgggtggcga caacgtgatc ctggagggcg tggcccacct
    11161 ggacgacggg gccccgccgg ccgaccgggt ctccgagtac ctgcgcaagt accgggaccg
    11221 gatcgcccag ggcgggtgga cgccggcgag cttcgcctcg gactacaccg tcccggtccg
    11281 gatcgacccc acccggatcc gggtctggtg aggccatgcg ggcgctggtg gcctcgggcg
    11341 gtggggttga gctccgccag gtgcccgagc cggagaccgg ccccgaccgg gcgctggtcc
    11401 gggtccgatc ggtctccctg aaccgggggg agtgcgccgc cctccgcacc gccgaggacg
    11461 gctggcgacc cggatgggac ctcgccggcg aggtcctccg ggcggccgga gatgggaccg
    11521 gccctcccga gggcgcccgc gtcgtggggt gggtgaacgg cggatcgtgg gccgagctcg
    11581 cggccgtgcg gaccgatcac ctggcgccgg tccccgatga gctggagccc gaggtgagct
    11641 cgaccctgcc ggtggctggc ctgaccgctc tcggcaccct ggcgctcggg gcaccgctcc
    11701 cggggcgccg ggtggccgtg accggcggag cggggggcgt ggggcggttc gcggtccagc
    11761 tggcccaccg ggacggcgct cacgtcaccg ctgtggtggg ccggccggag cgggccgagg
    11821 gcctgcacga gctcggggcc gacgagatcg ttgtgggcct ggagcccgag ggtcctgcct
    11881 tcgacctgat cctggagtcg gccgggggag catcgctggc ggcggccgtg agccgggtct
    11941 cggccgacgg gatgatcgtc accttcggga actcctcgaa cgagcccgtc accttcgacc
    12001 cacgaacgct gtaccgcaag ggtggacccc ggatcgtggg cttcttcgtg acctggctcc
    12061 tggagcgcgg gcgtatcggt gctcgggagc tgggcttcct ggcccggctg gtcgcgctcg
    12121 gccagctccg ctccgaggtc gccctgagga ccgactggtc ggacgccgcc tcggcgatcg
    12181 gtgcgctgat ggaccgcctg gtcgacggca aggcggtcct gtccatcggg tagcgctgcc
    12241 ggctccggga ccgagctccg ccgccgcgcc cggaggactc aggtgacgtg ggccacgtgc
    12301 tcgtcgagcg ggtcgtcgcc gaccggggcc agttcctcca cctccagggt gagcgcaggg
    12361 gcgatcgcgc ccaccgcccc gagtcccgcc cacagcggct cgagcatgcc cttggggacc
    12421 tcatcgccga tcgggatctc caggagcgtg tggtcctcgt ccgtcttcac gatgatccgg
    12481 cggacgacac ggccggccat cagccgcttg accgtgacga cgagctgctc gccggtgacg
    12541 cggatgctcc gccagcggac ctcgcgttcc atgtcggccc tcctcgacgg ccagtatcga
    12601 tcccagcgcg ggttgggcga ggggcccccc ggaccgcggt tcgcggaccg accgacccat
    12661 gtgggtcaaa tcgtgagctc gccgcacatc tcgtcgaaca cggcggtgtg tcggtccacg
    12721 tcctccgccg tggtggccgg cgacatcagg gccatgttgt ggaagggcgt gagcagcacg
    12781 ccccggttca acgcgtacag gtgggtgaac cgagccagct cgtgatcgtc ggccgccgcc
    12841 gcccggccgc ccgtggtggg gggctccggg cagaaccagt actcggcccg gcaccccagc
    12901 cgctgcacct cccagggcag gccgtgggca tcgatgccgg ccttcacgcc ctccgcccat
    12961 cgagcgccga gctcgatcat ccgctcgaac gcctcgtccg tcaggacgtt ctcgagcgtg
    13021 gccctcatgg ccgccagcga caacgcgttc cccgagaggg tcccgcccac gccgcccaca
    13081 tccgtggagg gcagaccgtc catgtaccac tggacccgcg aggccacctc ctcgctcatc
    13141 ccgtagcagg cggcggggac gccgctggcg agcggcttgc cgatcgtgag gaagtccggc
    13201 tccaggcccc aggccttggt ggcgccgccg gggcccgcgc agatcgtgtg ggtctcgtcg
    13261 atcaccaggt acgtgccgta gcgacgggtg agctcgcgca gggtctcgtg gtacccggcc
    13321 tgggggagca cgatgccgat gttcgtgagg gccggctcgg ccagcacgca ggccacgtcc
    13381 tcgtgcgcca gggcctcctc gagcgcggcc gggtcgttga actcgaccac ccgggtggtc
    13441 atggccgggt ccaccgcggg cccgatgttc cccggccgga tcgccgatcc ggtcccgtcg
    13501 tcggtggtga cgatgatcgt ctcgtccacc gagccgtggt ag
//
LOCUS       DASQQQ010000360         9229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_238389, whole genome shotgun sequence.
ACCESSION   DASQQQ010000360 DASQQQ010000000
VERSION     DASQQQ010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9229
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238389"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VE646_13480"
     CDS             complement(<1..552)
                     /locus_tag="VE646_13480"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013921048.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HZD81036.1"
                     /translation="MRRQDPPSAEGTIRDRSRPGRRAYSLPALDVPERDLPPDQTRVE
                     PPDLPEVAERDLVAHYTRLAQRNYGVDTGMYPLGSCTMKYNPKLAEAAAALPGFQRVH
                     PLQPASTVQGALELLWRLERALCEITGTARATLQPPAGACGELTGLLIMRAHHTGQGD
                     PRRRVAIPDSSHGTNPASVRMAGY"
     gene            complement(564..1889)
                     /gene="gcvPA"
                     /locus_tag="VE646_13485"
     CDS             complement(564..1889)
                     /gene="gcvPA"
                     /locus_tag="VE646_13485"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HZD81037.1"
                     /translation="MRFAPHTDEDVREMLARCGLGSLDALFSQIPASVRLERPLDLPE
                     GVSEMEVLADLRSLAARDRGTEDLVCFAGGGAYDHYVPAVVWSLAGRSEFYTSYTPYQ
                     PELSQGVLQALFEYQSMICELTGMDVSNASLYDGATALAEAVNLARSAPGRGRVLVSG
                     AMDPRYVETLRTYGRGTGYEPEIFDVPDGRGGDPPLGPDVACLIVQHPNVYGLLEPAR
                     ELFGAAHAAGARAIQVFDPLSLGVLAPPGDLRADIAVAEGQVLGNHLSFGGPYLGIIA
                     TRLDDVRRLPGRIVGETLDVDGRTGYVLTLQAREQHIRREKATSNICTNQTLMAIAAT
                     VYLAWLGPQGLAELGRQCAARAAYALDRLTRIRGVEPMFPGGECFKEFVLRLPRPASS
                     VVEDLVARGFLAGVPLPQGDGHGLLVAVTEQRTRDQIEGLADAMGEVLR"
     gene            complement(1947..2321)
                     /locus_tag="VE646_13490"
     CDS             complement(1947..2321)
                     /locus_tag="VE646_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81038.1"
                     /translation="MTTASTGAALPALKSDTVRFAHASEQQFARLLDFYQIEWEYEPT
                     SFDLEWDKHGAVIQRFTPDFYLPQYDLYIEITTLNQKLVTKKNRKVRRLRELYPGVNC
                     KIFYQRDYLSLVTKYGLEDVSG"
     gene            complement(2318..2767)
                     /locus_tag="VE646_13495"
     CDS             complement(2318..2767)
                     /locus_tag="VE646_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZD81039.1"
                     /translation="MAEQGYRVPEVCRIVGISYRQLDYWARTGLVRPSVKDAQGSGTQ
                     RLYSFRDLATLKLIKRMLDSGVNLQQIRKAMGTLKELKEPALGTTLVSDGTRIYSVES
                     PEAVVDLLAKGQGVFAIAVDRVWTELEGSLAKSDKKRGRPVARAGGV"
     gene            2900..3385
                     /locus_tag="VE646_13500"
     CDS             2900..3385
                     /locus_tag="VE646_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZD81040.1"
                     /translation="MVREGAQRSPVARRRFLIVILVTALTTLLAVGPATAKTPRKRLF
                     AMLNAARQSHHRRPVRFSLKLSDTAKQHTRRMVRRDQLFDVPNLSQVLAPFNWSWGGS
                     VEGCGSTLWGIHKAMMHHAAHRRIMLAPEARRVGIGVVKTTDRTSCGRNVFWVTEILY
                     G"
     gene            complement(3409..3882)
                     /locus_tag="VE646_13505"
     CDS             complement(3409..3882)
                     /locus_tag="VE646_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HZD81041.1"
                     /translation="MIEMNLAGVRIELPTNQPIVLLREAEGERFLPIWIGQAEAAAIA
                     LAMQGVVTPRPMTHDLLKNILQEISVQVQSIVITELREGTFYAIINMQRNGTSFEISS
                     RPSDAIALAVRIQCKIFANEEVLNEASILIPSGDEEEEVEKFREFLENVNPEDFG"
     gene            complement(3926..4564)
                     /gene="hpt"
                     /locus_tag="VE646_13510"
     CDS             complement(3926..4564)
                     /gene="hpt"
                     /locus_tag="VE646_13510"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HZD81042.1"
                     /translation="MSEVASVLFGREDIRRRTWELGRTITGDYEGRAPVLISVLKGGS
                     LFLADLIRQVRLPLSVDYMSISRYGGAAESMGRVRIVKDLDEDIGGRDVLVVEDIIDT
                     GLTLSYLISVLESRLPASVQVCTLLDKSVRRIAPLEIRYRGFDCPDVFVVGYGLDFHE
                     RYRNIPDILAIHDIPALEADPDALLPFLTGPEEGPVPPAGEKVAPSTEEAGG"
     gene            complement(4591..5856)
                     /locus_tag="VE646_13515"
     CDS             complement(4591..5856)
                     /locus_tag="VE646_13515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HZD81043.1"
                     /translation="MSSSRAARRIAPWLVLLVLIAAPGLAAAASSVPPPTPVPVPGGG
                     TSPSPFPTALATPSPSTEPPTLHAASAALIDVDSGQVLFQQAGRAHRPIASLTKIMTA
                     LLVLQRSDPQEPVVVGADAAVPRQTVGVSQLGLREGERITVEELLYALLLQSANDAAV
                     ALADHVAGSVDAFVAAMDARARRMGLRDTRFASPNGLDDAGYSSAIDLARLTRVALRQ
                     PLFAKVVATKFHDIPGPGRGVRRVQNRNVLLWLYPGASGVKTGYTSAAGFCVVAAAER
                     DGLRLVAVVLGEPGEPFSDAAALLDFGFTSFEHRALVTGRTGMGTVRIDGREVDVSTA
                     RGLSGLVPTDAPLRRRIVVDPGVRFPPLVGDRVGTLQIWAGDLRLGNVPLLATRVPPP
                     PPPDPGPWWRRGAGSVARAVAGILSALFD"
     gene            complement(5868..6575)
                     /locus_tag="VE646_13520"
     CDS             complement(5868..6575)
                     /locus_tag="VE646_13520"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZD81044.1"
                     /translation="MPTRNYQSIGEILVSVKTEFPDITISKIRFLESEGLIQPERTPS
                     GYRKFYGKDVDRLKDILRMQRDEYLPLKVIKERLLQQEAGEPEVEVASEEGEKEAGEE
                     LAEPPTGLQMSLEEMSAATGVEKERIEELESFGLVCRHGPESARYYDGDDYVVLSIVK
                     DFFRHGVEPRHLTMYRHFAEREAAFFETIVMPQLRQRNPEARKLATASLTELARASRK
                     LKQALLRNNLRQYLATP"
     gene            complement(6581..7039)
                     /locus_tag="VE646_13525"
     CDS             complement(6581..7039)
                     /locus_tag="VE646_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZD81045.1"
                     /translation="MYCTRCGHPNKDGARFCANCGAPLQDDSTLGIAPVEVEEEAGEE
                     FPFPGEHLDPGQGLLMVTRGPNAGSTFLIEKDVTTAGRNTQSDVFLDDVTVSRKHAEI
                     RRSGSEFFIHDVGSLNGTYVNRERVDMSKLASGDELQIGKFRLTFFRAEE"
     gene            complement(7185..7580)
                     /gene="gcvH"
                     /locus_tag="VE646_13530"
     CDS             complement(7185..7580)
                     /gene="gcvH"
                     /locus_tag="VE646_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HZD81046.1"
                     /translation="MEFPEDLRYTKEHEWVRDEGNGRVRMGITDYAQDALGDVVYVDV
                     PEVGTAVTASEPFSEVESTKSVSDVYGPVSGTVVERNPLLDERPELVNESPYDQGWLV
                     VLEVAEASGLGSLMDAAAYQRFVQEAGEG"
     gene            complement(7630..8253)
                     /locus_tag="VE646_13535"
     CDS             complement(7630..8253)
                     /locus_tag="VE646_13535"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZD81047.1"
                     /translation="MTGRLGQTGSPQASKALLTVPNVLSMARIVLIPVFVLLIVHRPT
                     TFSGLVLFAVVVATDWVDGAIARRIGQVSELGKILDPTADRLAIAAGLVALVVRGAFP
                     LWAALLVLVRDGLVLGVASAAIFMRQVRIDVRTVGKVATFGVLLAIGSISWGTLGYAF
                     ATGFLAFGWTCFGVGILEYYVAMGLYVGDLRRALAARGADVSGPARA"
     gene            complement(8590..8664)
                     /locus_tag="VE646_13540"
     tRNA            complement(8590..8664)
                     /locus_tag="VE646_13540"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8627..8629),aa:Pro,seq:ggg)
     gene            complement(8723..>9229)
                     /locus_tag="VE646_13545"
     CDS             complement(8723..>9229)
                     /locus_tag="VE646_13545"
                     /inference="COORDINATES: protein motif:HMM:NF026064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81048.1"
                     /translation="VLDASEGMTGEDRRIAARVMDLGRGLTIVANKWDLVDERDRTYQ
                     QLARAITVFAGATVTRTTATTGAGIGRLPDGLLELDRRWSTRVPTGEVNRLLGAAQEE
                     RPPPGGVRYRYATQVAAGPPTFILFGGRPPDRSYQRFLENRLRRELGLEGIPIRLRFR
                     PKPPRAGA"
BASE COUNT         1421 a         3234 c         3155 g         1419 t
ORIGIN      
        1 gtagcccgcc atgcgcacgc tggccgggtt ggtcccgtgc gaggagtcgg ggatcgccac
       61 ccgccggcgc gggtcgccct gccccgtgtg gtgcgcccgc atgatcagca gaccggtcag
      121 ctctccacag gccccggcgg gaggctgcag ggtggctcgt gccgtcccgg tgatctcgca
      181 tagcgcgcgc tcgaggcgcc acagcagctc cagcgctccc tggacggtgg acgccggctg
      241 cagcggatgc acgcgctgga acccgggcag ggccgccgcg gcctcggcca gcttggggtt
      301 gtacttcatg gtgcaggagc ccagcgggta catgcccgtg tccaccccgt agttgcgttg
      361 cgcgaggcgc gtgtagtgcg cgaccaggtc ccgctccgcg acctcgggca ggtccggcgg
      421 ctcgacccgg gtctgatcgg gtggcaggtc ccgctcgggc acgtcgagcg cgggcaggct
      481 gtacgcgcgc cggcccggcc gcgagcgatc cctgatcgtt ccctcggcgg agggtggatc
      541 ctggcggcgc accggtgcgc ccctcacctc agcacctccc ccatcgcgtc cgcgagccct
      601 tcgatctggt cccgggtacg ctgctccgtc actgccacca gcaggccgtg gccgtcgccc
      661 tggggtagcg gcaccccggc caggaaccct cgcgccacca ggtcctccac caccgatgac
      721 gccggccggg gcagccgcag gacgaactcc ttgaagcact caccccccgg gaacatgggc
      781 tccacgcccc ggatccgggt caggcggtcg agggcgtacg cagcgcgggc cgcgcactga
      841 cgccccaact ccgcgagccc ctgcgggccg agccatgcca ggtagacggt cgcggcgatc
      901 gccatgaggg tttggttcgt gcagatgttc gatgtggcct tctcccgccg gatgtgctgc
      961 tcccgcgcct gcagcgtgag cacgtagccg gtgcgaccgt ccacgtccag cgtctctccc
     1021 acgatccgcc ccgggagccg tcgcacgtcg tccaggcggg tggcgatgat ccccagatac
     1081 gggcccccga agctcaggtg gttgccgagg acctggccct cggccaccgc gatgtccgcc
     1141 cggaggtccc cgggaggggc caggaccccc agcgacagcg gatcgaagac ctggatcgcc
     1201 cgagcgccgg ccgcgtgggc ggctccgaac agctcccggg ccggctcgag cagcccgtag
     1261 acgttcgggt gctggacgat caggcaggcg acgtccggac cgaggggcgg gtcgcctccc
     1321 cggccgtcgg gcacgtcgaa gatctcgggc tcgtacccgg tccctcgccc ataggtgcga
     1381 agggtctcca cgtaccgcgg gtccatggcc cccgagacca ggacccgtcc acgtccgggg
     1441 gccgaccggg ccaggttcac cgcctccgcc agcgccgtgg caccgtcgta cagggaggcg
     1501 ttcgacacgt ccatcccggt cagctcgcag atcatggact ggtactcgaa cagggcctgg
     1561 agcacgccct gtgacagctc gggctggtac ggcgtgtacg aggtgtagaa ctcggagcgt
     1621 ccggcgagcg accacacgac ggcgggcacg tagtggtcgt acgcgcctcc tccggcgaag
     1681 cacaccaggt cctcggtgcc gcggtcgcgg gcggccagcg agcgcaggtc ggccaagacc
     1741 tccatctcgg agacgccctc cggcagatcc agcggacgct ccaagcgaac ggaggcgggg
     1801 atctgcgaga agagtgcgtc cagggaaccg aggccgcagc gggccagcat ctcgcgtacg
     1861 tcctcgtcgg tgtgcggcgc gaacctcatg gctccggatg ccatcctagc gatgcgttcg
     1921 cgatccgccc gatgacacgg gcgtgctcag ccggagacgt cctccagccc gtacttcgtc
     1981 accagggaga ggtaatcccg ctggtagaag atcttgcagt tgacgcccgg gtacagctcg
     2041 cggagcctgc ggaccttccg gttcttcttc gtcacgagct tctggttcag ggtggtgatc
     2101 tcgatgtaca ggtcgtactg gggcaggtag aagtcgggcg tgaaccgttg gatcaccgcc
     2161 ccgtgcttgt cccactcgag gtcgaaggac gtgggctcgt actcccactc gatctggtag
     2221 aagtccagca accgggcgaa ctgctgctcg gaggcatggg cgaatctgac ggtgtcgctc
     2281 ttgagggccg gcaacgctgc gccggtgctg gcggtggtca cacgcctccg gcccgagcga
     2341 ccgggcgacc ccgcttcttg tcgctcttcg cgagacttcc ctcgagttcc gtccataccc
     2401 tgtccacggc gatggcgaag accccctggc ctttggccag caggtccacc acggcctccg
     2461 gcgactccac cgagtagata cgtgtgccgt ctgagacgag ggtggtgccg agcgccggtt
     2521 ccttcagctc cttgagcgtg cccatcgcct tgcggatctg ctggaggttg acccccgaat
     2581 ccagcatccg cttgatgagc ttgagggtgg ccaggtctcg gaacgagtac agcctctgcg
     2641 ttcccgagcc ctgggcatcc ttcacggagg gccgcaccag ccccgtgcgg gcccagtagt
     2701 cgagctgccg ataactgatg ccgacgatgc gacatacctc ggggacgcgg taaccttgct
     2761 cggccatgac atctccgtaa ttacgtagac gttcgtcagg ctagttcccc gccgcacgag
     2821 tgtcaatcac cccagggagg cgacgggaac cgcatgcggc ctctgagggg gcttgacagg
     2881 tccgctacta tccggggccg tggtccggga aggcgcccag cgttcgcccg tcgcacgtcg
     2941 gcggttcctg atcgtcatcc tggtgaccgc cctcacgacc ctgctggccg tcggtcccgc
     3001 cacggccaag acgccccgca agcggctctt cgcgatgttg aacgccgccc gtcagtcgca
     3061 ccaccgtcgc ccggtgaggt tcagcctcaa gctctcggac accgcgaagc aacacacgcg
     3121 ccggatggtg cgccgggatc agctcttcga cgtgcccaac ctctcccagg tgctggcccc
     3181 cttcaactgg tcctggggag ggagcgtgga gggctgcggg tcgaccctgt gggggatcca
     3241 caaggcgatg atgcatcacg ctgcgcaccg gcggatcatg ctggctccgg aggcccggcg
     3301 ggtgggcatc ggcgtggtga agacgaccga caggacctcg tgcggtcgga acgtcttctg
     3361 ggtgaccgag atcctctacg gctgagcccg ggggtcagct cagcaggatc agccgaagtc
     3421 ctcgggattc acattctcca ggaactcccg gaacttctcg acctcctcct cctcgtcccc
     3481 gctggggatg aggatcgagg cctcgttcaa gacctcctcg ttggcgaaga tcttgcactg
     3541 gatccgtacg gccagcgcga tggcatcgga cggccgcgag gagatctcga acgacgttcc
     3601 gtttcgctgc atgttgatga tggcgtagaa ggttccctct cgaagctccg tgatcacgat
     3661 gctctgcacc tgcacggaga tctcctggag gatgttcttg agcaggtcgt gggtcatggg
     3721 gcggggggtg accaccccct gcatggccag cgcgatggcg gcggcctccg cctgtccgat
     3781 ccagatcggg aggaaccgtt ctccctccgc ctcccgcaag agcacgatgg gctggttcgt
     3841 ggggagctcg atgcggacac cggcgaggtt catctcgatc atggctcagt caccgagagt
     3901 atccccgggc atctgctccg gcaacctacc cgccggcctc ctcggtggac ggggcgacct
     3961 tctcccctgc cggtgggacc ggtccttcct ccggcccggt gaggaagggc aggagagcat
     4021 cgggatccgc ctccagcgcg gggatgtcgt ggatggccag gatgtccggg atgttgcggt
     4081 accgctcgtg gaagtcgagc ccgtagccca cgacgaacac gtcggggcaa tcgaaccccc
     4141 ggtaccggat ctcgagggga gcgatgcggc gcacggactt gtcgagcagc gtgcacacct
     4201 gcaccgaagc cggcaggcgc gactccagca ccgagatgag gtaggagagc gtgaggccgg
     4261 tgtcgatgat gtcctccacc acgaggacgt cccttcctcc gatgtcctcg tccaggtcct
     4321 tgacgatccg cacccggccc atggactcgg cggctccccc gtaccgagag atggacatgt
     4381 agtcgacgga caggggcagg cgaacctgcc ggatcagatc ggcgaggaac agggaaccgc
     4441 ccttcaggac cgagatgagg acgggcgcgc gcccttcata gtccccggtg atggtgcggc
     4501 cgagctccca ggtccgccgg cggatgtcct cgcgtccgaa caagacgctg gcgacctccg
     4561 acatcccggg taccgatcgt agcagaggga tcagtcgaac aaggccgaca agatgccggc
     4621 cacggccctg gccaccgatc cggccccccg acgccaccat ggccccggat ccgggggcgg
     4681 tggcggtggg acccgcgtcg ccagcagcgg aacgttcccc aggcggagat cgccggccca
     4741 gatctgcagg gtccccacgc ggtccccgac caagggtggg aagcggaccc cggggtccac
     4801 cacgatccgg cgccgcaggg gagcatcggt gggaacgagg cccgagagcc cccgcgccgt
     4861 ggaaacgtcc acctctcgcc cgtcgatgcg caccgtcccc atcccggtcc tcccggtcac
     4921 caacgcgcgg tgctcgaacg aggtgaaccc gaagtcgagg agggccgccg catcggagaa
     4981 gggctcgcct ggctccccca ggaccaccgc caccagccgg agcccgtccc gttcggccgc
     5041 ggcgaccacg cagaacccgg cggcgctcgt gtatccggtc ttcacccccg aggcgcccgg
     5101 atacagccac aacaggacgt tgcggttctg cacccgccgc acccctcttc ccgggcccgg
     5161 gatgtcgtgg aacttcgtcg ccaccacctt ggcgaacagc ggttggcgca gggcgacgcg
     5221 ggtcaggcgg gccaggtcga tggcgctcga gtagcccgcg tcgtccaggc cgttgggcga
     5281 ggcgaaccgg gtgtcgcgca gccccatccg tcgggccctg gcgtccatcg ccgccacgaa
     5341 cgcgtccacc gatcccgcga catggtcggc gagcgccacg gcggcgtcgt tcgccgattg
     5401 gagcagcagg gcgtacagga gctcctcgac ggtgatccgc tcgccctccc tcaggcccag
     5461 ctgggagacg ccgacggtct ggcgggggac ggccgcgtcc gcccccacca cgaccggctc
     5521 ctgggggtcg gagcgctgga ggaccaggag cgcggtcatg atcttcgtga ggctggcgat
     5581 cggtcgatgg gcccgcccgg cctgctggaa caggacctgg ccgctatcga catcgatcag
     5641 ggcagccgag gccgcgtgca gggtgggcgg ctccgtggag ggcgatgggg ttgccagcgc
     5701 cgtggggaac ggtgagggtg aggtcccgcc gccggggacc ggcaccggcg tagggggcgg
     5761 gaccgaggag gcagcggcgg cgagcccggg cgccgcgatc aggaccagga gcacgagcca
     5821 gggggcgatg cggcgggcgg cccgggagct cgacatcgga tggacctcta gggggtcgcc
     5881 aggtactggc ggaggttgtt ccgcagcagg gcctgcttca gcttccgcga ggccctggcc
     5941 agctcggtca gggaggcggt ggccagcttg cgggcctcgg ggttccgctg ccggagctgg
     6001 ggcatcacga tggtctcgaa gaaggcggcc tcgcgctcgg cgaagtgtcg gtacatcgtg
     6061 aggtgccggg gctccacgcc gtgacggaag aagtccttga cgatcgacaa caccacgtag
     6121 tcgtccccgt cgtagtagcg ggccgactcg gggccgtgcc gacagacgag gccgaaggac
     6181 tcgagctcct cgatccgctc cttctccacg ccggtcgcgg cggacatctc ctcgagtgac
     6241 atctgcagcc cggtgggcgg ttccgccagc tcctcccccg cttccttctc cccctcctcg
     6301 gatgccacct cgacctcggg ctctcccgcc tcctgctgca gcagtcgctc cttgatgacc
     6361 ttcaagggca ggtactcgtc gcgctgcatc cgcaggatgt ccttcagccg atccacgtcc
     6421 ttgccgtaga acttgcggta gccggagggg gtccgctcgg gttggatcaa tccctcggac
     6481 tcgaggaagc ggatcttcga gatcgtgatg tcggggaact cggtcttcac cgagaccagg
     6541 atctcgccga tcgactggta gttgcgggtg ggcatgctcg tcactcctct gcccggaaga
     6601 aggtcagccg gaacttgccg atctgaagct cgtcccccga ggccagcttc gacatgtcca
     6661 cgcgctcgcg gttgacgtag gtcccgttca ggctcccgac gtcgtggatg aagaactcgc
     6721 tccccgatcg ccggatctcc gcgtgcttgc gcgaaacggt cacgtcgtcg aggaagacgt
     6781 cgctctgggt gttccggccg gccgtggtga cgtccttctc gatcaggaag gtggagcccg
     6841 cgttcggccc gcgggtgacc atcagcaggc cctggccggg atccagatgc tctccgggga
     6901 aggggaactc ctctcccgcc tcctcctcga cctccaccgg agcgatgccc agggtgctgt
     6961 cgtcttggag gggagcccca cagttcgcgc agaagcgggc cccgtccttg ttcggatgtc
     7021 cacaccgggt gcagtacaag ctcgctcgct cctcggggcg aagctcgcgc tcaacgtgga
     7081 ggttggcgcc gagtctcgac cctcgaccga agctggacgt tagcgcagcg cggcagcagg
     7141 gtcaaccgac cgcgggagga gcggtggtgc gcaaggacca ccggctaccc ttcacccgcc
     7201 tcctgcacga accgctggta cgccgccgca tccatgagcg atcccaagcc gctcgcctcg
     7261 gccacttcca ggaccacgag ccagccctgg tcgtagggcg actcgttcac cagctcgggc
     7321 cgctcgtcga gcagggggtt gcgctccacc accgtgccgc tgaccggccc gtacacgtcc
     7381 gagaccgact tggtggactc cacctcgctg aatggctccg aggcggtgac ggcggtcccg
     7441 acctcgggga cgtcgacgta gaccacgtcg cccaacgcgt cctgcgcgta atcggtgatg
     7501 cccatccgga cccggccgtt cccctcgtca cggacccact cgtgctcctt cgtgtaccgg
     7561 agatcctcgg ggaactccat cgtctcctcc ctcgatcagc ggcgtgcggg cgtcgcggca
     7621 gtgaccgtat cacgcgcgag ccggcccaga gacgtcggcg ccgcgagcgg ccagggcccg
     7681 tcgcaggtct ccgacgtaca ggcccatggc gacgtagtac tcgaggatgc cgacgccgaa
     7741 gcaggtccaa ccgaaggcca ggaatccggt ggcgaacgcg tagccgagcg tcccccacga
     7801 gatcgatccg atggccagga gcacgccgaa cgtggccacc ttgcccaccg tgcgaacgtc
     7861 gatccggacc tgacgcatga agatcgccgc cgaggccacc ccgagcacca agccgtcacg
     7921 gaccagcacc agcagggccg cccagagggg gaaggcacct cggaccacca gagccaccag
     7981 cccggcggcg atggcgagtc ggtcggcagt cgggtccagg atcttcccca gctccgagac
     8041 ctgaccgatc ctgcgggcga tcgcgccgtc cacccaatcg gtggcgacca ccacggcgaa
     8101 caggacgagt ccggagaacg tcgtcggtcg atgcacgatg agcaggacga agaccgggat
     8161 cagcacgatc ctggccatcg acaggacgtt gggcaccgtc aggagcgcct tcgatgcctg
     8221 cggtgagccg gtctgaccaa gacggcccgt catggcgcgg aacctatcac ggcgcccgcc
     8281 gatcccaggt actaggctgc gcggccatct tgacctagac taggggctcg ccggaccgag
     8341 gcctccatgc gcaccgaagc cttcggtaac ggctcagggt ggcccgggct cttcctcccc
     8401 gggccacctg tctgtgtggg ccatccgcac ggcgggctcg accttgggag tccctcccgc
     8461 tccagtggag ggtcggcccg gggcatcatc agacacgact cgagggtcgc cgtgcttggg
     8521 tgacctcatg gggacctggc gttcgggcgg gaggggacct ggagggacgc gggcgtgcgc
     8581 tccggtcggt cgggccggcc ggatttgaac cggcgacctt ctgaccccca gtcagacgcg
     8641 ctaaccaagc tgcgccacgg cccgtgcgat cgattgtacc gggcctcgcc acccgcggac
     8701 cggcctcgct cccctcgctc gctcaggctc ctgcgcgcgg tggtttcggg cggaagcgga
     8761 gcctgatcgg gatcccctcc aaccccagct cccggcgcag gcggttctcc aggaagcgct
     8821 ggtaggaccg atccggggga cgaccgccga acaggatgaa ggtgggcggg ccggccgcca
     8881 cctgggtggc gtaccggtac cgcacgccgc cgggcggcgg acgctcctct tgcgccgcgc
     8941 cgaggaggcg gttcacctcc cccgtcggga cacgcgtcga ccatcggcgg tcgagctcga
     9001 ggagcccgtc cgggagccgg ccgatgcctg cgcccgtcgt ggccgtggtc cgtgtcacgg
     9061 tggccccggc gaagacggtg atcgcccgag cgagctgctg gtaggtgcgg tcccgctcgt
     9121 ccacgaggtc ccacttgttg gcgacgatcg tcagcccgcg accgagatcc atcacccgcg
     9181 cggcgatcct ccgatcctcg ccggtcatcc cctcgctcgc gtccaagac
//
LOCUS       DASQQQ010000361         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_53727, whole genome shotgun sequence.
ACCESSION   DASQQQ010000361 DASQQQ010000000
VERSION     DASQQQ010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53727"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..1031
                     /gene="sigJ"
                     /locus_tag="VE646_13550"
                     /pseudo
     CDS             237..1031
                     /gene="sigJ"
                     /locus_tag="VE646_13550"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
     gene            1122..2114
                     /locus_tag="VE646_13555"
     CDS             1122..2114
                     /locus_tag="VE646_13555"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZD81049.1"
                     /translation="MDGAARSTTTHGGEELEALELHRAELTGYAYRMLGSAFEAEDAV
                     QETMLRAWRALDRFEGRSSLRSWLYSIATNVCLDMLGGKERKARPMDLGPAGSADGPV
                     PDPLVDGAWILPVPDERVVPEDGDPAELTEARESVRLAFVAALQHLPPRQRAVLILRE
                     VLRWRASEVAELLETSVASVNSLLQRARATLASVDLENTDAFRPMDPDQRDLLARYVD
                     AFERYDMDALTSLLHRDATWNMPPYELWLRTHRDIRMWCLGPGIGCRGSRLIPTAANG
                     SPAFGQYKPDPGGGLSPWSLQVLEISEGAIAGITFFLNTQALFPLFGLPPHLDG"
BASE COUNT          328 a          786 c          834 g          346 t
ORIGIN      
        1 acgaagatcc tcatccccaa ctccttccac acttcctacc cttgagacga ggcagctccg
       61 ccgcccgtga caggcggacc ggtgtcacgg ggggagacgc tgcctcgtcg aggagggtga
      121 tgagcgaagc gcacaccgat accgcagatc ggaaccgcac cgaggacctg gagcggttcc
      181 gcccgctcct gttctcgatc gcctaccgga tgatcggcta ggtcggggac gccgaggatg
      241 tggtgcagga ggcgtacctg cgggcacatc gcgccgtcag cgatggaacg ccgatcctgc
      301 gacccaaggc gttcctggcc gaggtgacga cgcgcctggc gatcgaccac ctgcgctcgg
      361 cacgggtgcg gcgcgagcgg tacgtgggcc cctggctgcc cgagcccctg ctgaccgaga
      421 ccgaacccga cgtggcggac cgagcggaga tgctcgagtc gctctcgatg gccttcctgg
      481 tcgtgctgga gagcctgtcc cccgtggagc gggcggtgct cctgctccac gacgtgttcg
      541 ggttcgggtt cgacgaggta gcccggatcg tgggcaagag cgtggagaac tgccggcaga
      601 tcgcggtgcg cgctcgccgc aacgtcgagg cccgccggcc ccggttcgag gtctcacagg
      661 accggcagga cgagctggcc tcacggttct tcgcggcggc cgaggagggt gacgtcgact
      721 cgctggtgga gctgctggcc gaggacgtcg tgatggtcgg cgacggaggt ggccaagggg
      781 cacgccgcac gccggcccgc ggagccctgc gcgtggcccg gttcgtggtc ggcctggctc
      841 gcgcggctgc gcgccataac gtggtgaccg tgccggcgcg ggtcaacggc caggccgctg
      901 ccatcgccta ctggccggac ggctcggtcg cgagcgtcat gacggtggac gtggccgacg
      961 ggcgagtgat ggcggtcctc tcggtcgtga atccggacaa gctccggcac gtgccgccgc
     1021 tccctcgctg acccgctccg ggcggtgaga acgacctatc ggcgaggcgt cgtagagtgg
     1081 tggttcggtt cgtcgggaca cgatcagggt gggggacggc gatggacggc gccgcacgga
     1141 gcacgaccac gcacggagga gaggagctcg aggccctcga gctgcatcgg gcggagctca
     1201 cgggctacgc ctaccgcatg ctgggctcgg ccttcgaggc cgaggacgcg gtccaggaga
     1261 ccatgctgcg ggcgtggcga gccctcgacc ggttcgaggg acgctcctcg ctccggtcct
     1321 ggctctacag catcgccacg aacgtgtgcc tggacatgct cggcggcaag gagcgcaagg
     1381 ctcggcccat ggacctgggt cccgccggct ccgccgacgg tccggtgccc gatcccctgg
     1441 tggacggggc gtggatccta ccggtccccg acgagcgcgt cgttccggag gacggcgacc
     1501 cggccgagct gaccgaggcc cgggaatcgg tccggctggc gttcgtcgct gccctgcagc
     1561 acctgccgcc ccgtcagcgg gcggtgctga tccttcggga ggtcctgcgg tggcgggcca
     1621 gcgaggtcgc ggagctgctc gagacctcgg tggcatcggt caacagtttg ctgcagcggg
     1681 cccgagcgac cctggcgtcg gtcgacctgg agaacaccga cgcgttccgc cccatggatc
     1741 cggaccagcg tgacctgctg gcccgatacg tggacgcctt cgagcgctac gacatggacg
     1801 cgctcacctc gctgctgcac cgggacgcca cctggaacat gcccccgtac gagctgtggc
     1861 tgcgcaccca ccgcgacatc cggatgtggt gcctgggtcc cggcatcggg tgccgtggct
     1921 ctcggttgat ccccaccgcg gccaacggct cgccggcctt cgggcagtac aagccggacc
     1981 cggggggcgg cctctcgcct tggtccctgc aggtgctgga gatctcagag ggggccatcg
     2041 ccggcatcac cttcttcctg aatacgcagg ccctcttccc gctgttcggc ttgccacccc
     2101 atctggacgg ctgagggccg gtgcagggcc aggacttcgc tcagcccggt cagatccgcc
     2161 agcgcccgga gctccggccg gcatcggatg agccgaacct gggcgccgtg gcggagcgcg
     2221 gtgagctgca gccgacccaa cgtgtccagc acatccaggt cggccccgac gagacctccg
     2281 atgtcgcaca ggat
//
LOCUS       DASQQQ010000362         6089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_409434, whole genome shotgun sequence.
ACCESSION   DASQQQ010000362 DASQQQ010000000
VERSION     DASQQQ010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409434"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..834)
                     /locus_tag="VE646_13560"
     CDS             complement(85..834)
                     /locus_tag="VE646_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003995149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZD81050.1"
                     /translation="MDLSDTVAIVTGAARGIGRACVERLSREGAAVIATDVLDEEGEA
                     VLGSLVPENERLAYLHHDVSDESAWVEVVRSARERFGGLHALVNNAGIGSLADVEEET
                     REGWDHLIAVNQTGVFLGMKHAGPVMRETGGGSIVNMSSIFGAVGGFGGSIAYHASKG
                     AVRLMTKNAALRWAAEGVRVNSVHPGFVDTPMLDDIRDTEAMRQITDSTPMGRLARPE
                     EIAAVVAFLLSDDASYMTGSEVYVDGGWTGR"
     gene            940..2286
                     /locus_tag="VE646_13565"
     CDS             940..2286
                     /locus_tag="VE646_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HZD81051.1"
                     /translation="MASPKEVLQIAGRALSVSNPDKVFFPRTGHTKLDLVRYYLAVSE
                     GALGGVAGRPMALKRYVNGAEGEFFFQKRAPRSRPEWIETVELAFPSGRTAEEIVVRD
                     PAQLAWVVNLGCIDLNPHPVRADDLEHPDELRIDLDPVPGVGWPQIRDVALATGEALG
                     DLGLTGWPKTSGSRGIHIYCRIERRWTFPEVRRAALALAREVERRVPQLATSRWWKEE
                     RHGVFLDYNQNAKDRTIASAYSVRPTPDARVSAPLRWEEVPRCDPADFTIVSVPARLS
                     EQGDPWAGMEDAVGSLEPLLELAAEHEAAGFGDAPWPPHFAKQAGEPPRVQPSKRRRP
                     GSGPEGTVPPPPPGKSVGPTGRRRSRMPLIEVSRAATKEQAMEGLARWRDRHPEAWSR
                     LAPADVLIDAMRGRFTTWTRIRLNLRNVPPDQRPPQEPLEVDYDPWAGVRDERPDP"
     gene            complement(2326..3186)
                     /locus_tag="VE646_13570"
     CDS             complement(2326..3186)
                     /locus_tag="VE646_13570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017607077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZD81052.1"
                     /translation="MVVVTRDRRTRLMTTLAHLTALPEPLDVLVVDNASGDGTAGAVV
                     RRFPQVRVVRSPANLGAAARNIGVGSVSSPYVAFSDDDSWWEPGALARACDLFDDRPG
                     LGLIAGRILVGERGETDPTCLTMATSPLPSGAGSVGPSVLGFLACGAVVRRRAFLQVG
                     GFHPRLMIGGEERLLAIDLSVAGWVLAYVDDVVARHHPAPGPRAGRRRRETRNAVWTA
                     WLRRRALGAIGETMWAVRRAGDPEAALGLLDALGGWRWVLRERRPVPAGLDRALALVD
                     GWDGSASPSA"
     gene            complement(3219..3602)
                     /locus_tag="VE646_13575"
     CDS             complement(3219..3602)
                     /locus_tag="VE646_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017537496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HZD81053.1"
                     /translation="MPGMEDLPDTLERSPKKAQETWAKAHDSAVEQYGEGERAHRTAY
                     AALKHTFEKVGDHWEPKDGSGPSDPRAEKGQEEARQGKGETFGGIDLYGTTKEELYQR
                     AKKLDIGGRSKMSKKELARAIASRQ"
     gene            complement(3758..4720)
                     /locus_tag="VE646_13580"
     CDS             complement(3758..4720)
                     /locus_tag="VE646_13580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004923211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZD81054.1"
                     /translation="MTTPGERPTVLVLRALGLGDLLTAVPALRAVARAFPDHRRVLAT
                     PGRLAPLARHTGAVDAVLPAGPLQPLPGSARGPEVAVNLHGRGPQSHRLLLSLHPGRL
                     LAFANADVPGTEALPTWRADEHEVERWSRMLRAFGIAADPSDLDLDRPGLPSPIPAAG
                     ATVIHPGAGSAARRWPAERWARVAHRERAAGRRVVVSAGPGERRLAEDVARGPEAPAA
                     DVFVGDEDPMPLAALVAEAGRVASSDTGIAHLATALGTPSVVLFGPTPPALWGPPPDR
                     REHRVLWHGSTGDPLGDRPDPGLLRIGVDEVVRALAELPERHAG"
     gene            complement(4717..>6089)
                     /locus_tag="VE646_13585"
     CDS             complement(4717..>6089)
                     /locus_tag="VE646_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HZD81055.1"
                     /translation="SRLAPRLRVLRSGSRGPAAARNLGWRAGSAPWVAFLDDDVLPDP
                     DWLIRLQEDLQGAGPGIGGVQGRIHVPLPRHRRATDWERNVAGLEGAHWITADMAYRR
                     EALERVGGFDERFPRAYREDADLGLRVTAAGYRILRGTRRVQHPVRPAGPWVSVRLQR
                     GNADDALMLALHGWSWRRAAGVPRGARPLHLALSASGLVGLVGVLARRPVLAMVAIGG
                     WAAGTADLVRRRIAPGPRTPREIATMLLSSLAIPPAATVHWFVGLGRAARLRSGPGPV
                     PRVGHGPQPVEAVLFDRDGTLVTDVPYNGDPALVELLPDARRSVERLREGGIRVGVVS
                     NQSGVGRGLITTDQVERVNRRVEELLGPFDVWGVCPHGPQARCSCRKPAPGMVLEAMG
                     ALGARPSRTVLVGDTGADVEAGRRAGVRVILVPNAVTRVDEIVSAPEIASDLEVVADR
                     ILAGGR"
BASE COUNT          817 a         2376 c         2134 g          762 t
ORIGIN      
        1 ggccgagggg cggccgaggg gcggccgagg ggcggatgga cgtagccgac ctgctccgct
       61 acccggtcga acggggccag cgaactaccg gccggtccat cctccgtcca cgtagacctc
      121 gctccccgtc atgtacgagg cgtcgtcgga gagcaggaag gccaccaccg ccgcgatctc
      181 ctcgggccgg gccaagcgtc ccatcggcgt gctgtccgtg atctggcgca tggcctcggt
      241 gtcccggatg tcgtcgagca tcggcgtgtc cacgaagccc ggatgcaccg agttcacccg
      301 gaccccctcc gcggcccaac ggagcgccgc gttcttcgtc atgagccgga cggcgccctt
      361 ggaggcgtgg taggcgatcg aaccgccgaa ccctccgacc gcgccgaaga tcgagctcat
      421 gttcacgatg gagccgccgc ccgtctcgcg catcaccggg ccggcgtgct tcatccccag
      481 gaagaccccc gtctggttca cagcgatgag gtggtcccat ccctcccggg tctcctcctc
      541 gacgtcggcg agactgccga tccccgcgtt gttgaccaga gcgtgcagac cgccgaaccg
      601 ctcccgcgcc gagcgcacca cctcgaccca ggccgactcg tccgagacgt cgtggtgcag
      661 gtacgccaag cgctcgttct caggtacgag ggagccgagg accgcctcac cctcctcgtc
      721 cagcacgtcg gtggcgatca ccgccgcccc ctcccgggac agtcgctcca cgcacgcccg
      781 cccgatccct cgcgccgcgc cggtcacgat ggccaccgtg tcgctcagat ccaccctccc
      841 accgtttccg aggtccccgg cccggaaacg gcggtccggc cgcgaccccg ccgccccgtc
      901 gaggacacct gctcatcggc tcgcgcctat cgtgttgcca tggcatcgcc gaaggaggtg
      961 ctgcagatcg ccggtcgggc gctgagcgtg tccaatccgg acaaggtgtt cttcccgcga
     1021 accgggcata cgaagctcga cctggtccgt tactacctgg ccgtttccga gggtgccctg
     1081 ggcggagtgg ccggccggcc catggcgctg aagcgctacg tgaacggcgc ggaaggtgag
     1141 ttcttcttcc agaagcgcgc ccccagatcg cgccccgagt ggatcgagac cgtggaactg
     1201 gccttcccct cgggcaggac cgccgaggag atcgtggtcc gggacccggc gcagctggcc
     1261 tgggtggtga acctggggtg catcgacctg aacccccacc cggtgcgggc cgacgacctc
     1321 gagcacccgg acgagctgcg gatcgacctg gacccggtgc cgggggtggg ttggccgcag
     1381 atccgcgacg tggccctggc caccggcgag gccctcgggg atctgggcct gacgggctgg
     1441 cccaagacct cgggatcacg agggatccac atctactgcc ggatcgagcg ccggtggacg
     1501 ttccccgagg tgcggcgggc ggccctggcc ctggcccgcg aggtggagcg ccgggttccc
     1561 cagcttgcca ccagccggtg gtggaaggag gagcggcacg gggtcttcct cgactacaac
     1621 cagaacgcga aggatcgcac gatcgcgtcc gcctattcgg tccggcccac gccggacgcg
     1681 cgcgtctcgg ccccgctccg ctgggaggag gttccccgat gcgatccggc cgacttcacg
     1741 atcgtgagcg tgccggcccg gctctccgag cagggggatc cctgggccgg catggaggac
     1801 gccgtcgggt ccctggagcc tttgctcgaa ctggccgccg agcacgaggc ggccgggttc
     1861 ggtgacgccc cgtggccgcc ccacttcgcc aagcaggccg gtgagccgcc gagggtccag
     1921 ccctcgaagc ggcgacggcc cggatcgggg cccgagggca cggttccacc gcccccgccc
     1981 gggaagtccg tcggtcccac cgggcggcga cgcagccgga tgccgttgat cgaggtgtcc
     2041 cgggccgcca cgaaggagca ggccatggag gggctggcgc ggtggcgcga ccgccatccg
     2101 gaggcctggt cccgcctcgc cccggccgac gtgctgatcg acgcgatgcg aggccggttc
     2161 accacctgga cgcggatccg cctgaacctc cgcaacgtgc cgcccgatca gcgccctccg
     2221 caggagcccc tggaggtcga ctacgacccc tgggccggcg tgagggacga gcgtcccgac
     2281 ccctgaccca ccgagccccg acccaccggg atccgcgctc caacgtcagg cgctcgggga
     2341 cgcggatcca tcccagccgt ccaccagcgc gagggcgcga tcgagcccgg cggggaccgg
     2401 ccggcgctcc ctgagcaccc accgccaccc accgagggcg tccagcaagc cgagggcggc
     2461 ctcggggtcg ccggctcggc gaacggccca catggtctcc ccgatcgctc ccagggcccg
     2521 gcgccggagc catgcggtcc acaccgcgtt cctggtctcc cttcggcgtc gcccggcgcg
     2581 aggtccggga gccgggtggt gccgggccac cacgtcgtcc acgtacgcca gcacccagcc
     2641 ggccaccgac aggtcgatcg ccagcaaccg ttcctcgccg ccgatcatca ggcgggggtg
     2701 gaaccctccg acctgcagga acgcacgccg ccgaaccacc gcgccgcagg ccaggaaccc
     2761 aagtacggac ggacccaccg agcccgcacc cgagggcagg gggctcgtgg ccatggtgag
     2821 gcaggtcggg tcggtctcac cgcgctcgcc caccaggatc cgaccggcga tcagccccag
     2881 cccgggccga tcgtcgaaca ggtcacaggc ccttgccagg gctccaggct cccaccagga
     2941 gtcgtcgtcg ctgaaggcca cgtacgggct cgagaccgac cccaccccga tgttgcgggc
     3001 ggccgctccg aggttcgccg gcgagcggac cactcggacc tgcgggaagc gacgcaccac
     3061 ggctccggcg gtcccgtcgc ccgaggcgtt gtccacgacc agcacgtcga gcggctccgg
     3121 cagcgccgtc aggtgggcca gggtggtcat caggcgggtc cgtcggtccc gcgtcaccac
     3181 gaccacggcg caccgcggcc gacctgccgt cggcgcccct actggcggga cgcgatggct
     3241 cgtgcgagct ccttcttcga catcttcgac cggcccccga tgtccagctt cttggctcgc
     3301 tggtacagct cctccttggt cgtgccgtac aggtcgatgc cgccgaaggt ctcgcccttg
     3361 ccctgtcgag cctcctcctg gcccttctcg gcccgagggt ccgacggccc gctcccgtcc
     3421 ttcggctccc agtggtcccc gaccttctcg aacgtgtgtt tgagggcggc gtaggcggtg
     3481 cggtgggctc gctctccctc cccgtactgc tcgacggcgc tgtcgtgggc cttggcccag
     3541 gtctcctgtg ccttcttcgg cgaacgctcg agggtgtccg gcagatcctc catccccggc
     3601 atgcgacctc ctttcctccg tgtcgcgtcc ccgtccgtgc tccgggctgc gagccatccc
     3661 tacccaccgg cggcgagggg caggcggcga gcgtccccgg aggcggggac cgacacgaca
     3721 tcggtccccg gtgcccgggt ggccgaagga cggggcttca gcccgcgtgt cgctccggca
     3781 gctcggccaa cgcccggacc acctcgtcca ccccgatgcg gagcaggccc gggtcgggcc
     3841 ggtccccgag ggggtcgccg gtcgagccgt gccacaggac ccggtgctcg cggcgatcgg
     3901 ggggtggtcc ccacagggcc ggaggggtcg gaccgaacag cacgaccgag ggtgtgccga
     3961 gcgcggtggc caggtgcgcg atgcccgtgt ccgagctcgc cactcgtccg gcctccgcca
     4021 ccagcgcggc cagcggcatg ggatcctcgt cgcccacgaa cacgtcggcc gcgggcgcct
     4081 cgggtccacg ggccacgtcc tccgccagcc gacgctcgcc gggccccgcc gagaccacca
     4141 cccgccggcc cgccgcccgc tcccggtggg ccacccgagc ccaccgctcc gccggccagc
     4201 gccgggcggc ggagccggct ccggggtgga tcaccgtggc tccggcggcc gggatggggg
     4261 acggcagccc gggccgatcc agatcgagat cggaggggtc ggccgcgatg ccgaacgctc
     4321 ggagcatccg agaccagcgc tcaacctcgt gctcgtccgc ccgccacgtg ggcagggcct
     4381 cggtcccggg cacgtcggcg ttcgcgaagg ccagcagccg gccggggtgc agggacagaa
     4441 gcagccggtg gctctgcggg ccacgaccgt gcaggttcac cgccacctct ggcccgcggg
     4501 ccgatccggg gaggggttgc aggggacccg ccggcaggac cgcgtccacc gctcccgtgt
     4561 gcctggccag cggcgcgagc cggcccggcg tcgccaggac ccggcggtga tcggggaagg
     4621 cccgggccac ggcccggagc gcgggcacgg ccgtcagcag atcgcccagc cccagggccc
     4681 ggaggaccag gaccgtggga cgttcgccgg gcgtggtcac cggccgcccg ccaggatccg
     4741 gtccgccacc acctccaggt cggaggcgat ctcgggggcc gagacgatct cgtcgacgcg
     4801 cgtgaccgcg ttcgggacca ggatgacccg gacgcccgcc cgccgccccg cctcgacgtc
     4861 cgcgccggtg tcccccacca acacggtcct cgagggccga gccccgagcg cccccatcgc
     4921 ctcgagcacc atcccggggg cgggcttccg gcatgagcag cgcgcctgcg ggccgtgcgg
     4981 gcacacgccc cacacgtcga aggggcccag cagctcctcg acccgccggt tcacccgctc
     5041 cacctggtcg gtggtgatca gcccccggcc gaccccgctc tggttcgaga ccacgccgac
     5101 ccggatcccg ccctcgcgca gccgctccac gctgcggcgc gcatcgggca ggagctccac
     5161 cagggcgggg tcgccgttgt aggggacgtc cgtcaccagc gtgccgtccc gatcgaagag
     5221 caccgcttcc acgggctgcg gcccgtggcc aacccgcggg acggggcccg gcccggaccg
     5281 caaacgcgcg gcccgaccga ggcccacgaa ccagtggacg gtggccgcgg gggggatggc
     5341 caggctggac aggagcatcg tcgcgatctc tcgcggcgtc cgaggtccgg gagcgatgcg
     5401 tcgacggacc agatccgccg tccccgccgc ccatcccccg atcgccacca tcgcgagcac
     5461 cggtcgccgc gccaggaccc ccacgaggcc gaccagcccc gacgcgctca gggccaggtg
     5521 cagcggccgc gccccccgcg gcacccccgc cgcgcggcgc cagctccacc cgtgcagcgc
     5581 cagcatgagc gcgtcgtccg cgttgccccg ctgcagccgg acgctcaccc acgggccggc
     5641 cgggcggacc gggtgctgca cccggcgggt gcctcggagg atccggtacc ccgcggcggt
     5701 gacccgtagg ccgaggtcgg cgtcctcccg gtaggcacga gggaaccgct cgtcgaaccc
     5761 gcccacgcgc tccagggcct cccgccggta ggccatgtcg gccgtgatcc aatgggcacc
     5821 ctccaggccg gccacgttgc gctcccagtc ggtggctcgt cggtggcggg gcaggggaac
     5881 gtggatccgc ccctgcaccc cgccgatccc cgggccggct ccctggaggt cctcctgcag
     5941 gcggatcagc cagtcggggt ccgggagcac gtcgtcgtcc aggaaggcca cccagggggc
     6001 cgagccggcg cgccacccga ggttccgagc ggcggccggg cctcgggacc cactgcgcag
     6061 gacgcggagc cgtggagcca gccggctga
//
LOCUS       DASQQQ010000363         2915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_26536, whole genome shotgun sequence.
ACCESSION   DASQQQ010000363 DASQQQ010000000
VERSION     DASQQQ010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26536"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..462
                     /locus_tag="VE646_13590"
     CDS             91..462
                     /locus_tag="VE646_13590"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HZD81056.1"
                     /translation="MPLSEHEQHILEEIEARLAEEDPRLAAAVSRTSLYTHLARRIRW
                     ASIAFLLGFVMLMLFWASLWVAMAGFGVMLLSALLVYRYLKQLGRDQVRAIQQSGRFS
                     IAGLLTRLASRMRGDHRPPER"
     gene            685..1167
                     /locus_tag="VE646_13595"
     CDS             685..1167
                     /locus_tag="VE646_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZD81057.1"
                     /translation="MEGFTAHQATRFTGCTPRQLRYWDQIGLVKPSVQGTGGRPGVPR
                     LYSFRDLVALKVVKSLLDGGMSLQRVRRAWEYLNRRAALDRHLSEVKLITDGQSIFKV
                     ARRSGEVLDALKEGQLAFFVAIDEIATGVEARVGQFREDRDRFVRALREAAAHAQTGR
                     "
     gene            complement(1216..>2915)
                     /locus_tag="VE646_13600"
     CDS             complement(1216..>2915)
                     /locus_tag="VE646_13600"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HZD81058.1"
                     /translation="PIWTWHGRERAVTRSATAGARKVALVAVAVAVLLPGILPGFRSS
                     ALVDFSTTNPDRIGLDPFVSIKAQLQERTPINLFTVTSVDDQETPVAAYWRLYALDRF
                     NGVTWSSSDPLGAGGRVMSSPATFPSLLPSGLPPVHQTYRFLTDIPQPYLPMAYPPRT
                     IDLPIGPIHYDQNLISAVAGRDLNAGLEYTVTSGRLFPTPQQLDQVSFADPAAYLQDT
                     KVPDSVPDEVAQIARRWTAQAATPYEQVYTIEQRLLSSPFQYSLDVKPRADADALVDF
                     LTKTHRGFCQQFATAMATLVRELGIPARVVVGYLPGKQLDATFTVTTKQSHSWVEVLF
                     PGYGWLPFDPTPGTSKPTPPQGSYLSPDTSSSGPNRGGGETQTQGGRTTTTSDCGPGL
                     TSKLCTNPYGTARRPRGLGPDAIPALQPADAGRPSPYRRVVWVVLALAGMLALLFPLA
                     KKLWRRSLLRRIHDPRHAVLAAYRVFDGEATDLGLGRRQGETLAEHRRRLAGSVRFSD
                     GHLGRLTSMASTAAYSERSVSASDARAARRDARVAIQDLRRHASLSRRIAGVYRPGL"
BASE COUNT          423 a          971 c         1075 g          446 t
ORIGIN      
        1 cgtgaagcag gtacgctcgg tgggtaaaga cccttcaacc agaaccgatg tccttcctat
       61 aatggttttg ccgcaccgaa ggtaggagcg atgcctctct ccgagcacga gcagcacatc
      121 ctcgaagaga tcgaggcacg gctggccgag gaggatcccc gcctggcggc ggcggtgtcc
      181 cgcacatccc tgtacacaca cctggcgcgg cgcatccggt gggcgtccat cgcgttcctg
      241 ctcggcttcg tgatgctgat gctcttctgg gcttcgctgt gggtcgcgat ggccgggttc
      301 ggggtgatgc tgctgtcggc cctgctcgtg taccgctacc tgaagcagct cggacgcgac
      361 caggtccggg cgatccagca gtcgggacgg ttctcgatcg ccgggctcct cacccgcctc
      421 gcctcccgga tgcgtggcga tcaccgcccg cccgaacgct gatccgaaga acgctcgacc
      481 ggctcgcgag ttccgcgaca cgcgcgaacg gttcctccct cgccaccatc gtgcagcgcg
      541 cttgccactc gtccggtccc gcagtacaac atcgccactt cccccggggg cgcgaagccg
      601 tccggcgccg atcaccctgg cacgccggca gatcgccgct catcgttcga cggttcgacg
      661 ggtggtcgga ggacgcacgt gccgatggag gggttcacgg ctcaccaggc gaccaggttc
      721 acggggtgca cgccgcgcca actccggtac tgggaccaga tcgggttggt gaagccctcg
      781 gtccagggca ccggtggacg gcccggggtg ccccggctct actcgttccg agacctggtg
      841 gcgctgaagg tggtgaagtc cctgctggat ggggggatgt cgctgcagcg tgtccggcgg
      901 gcctgggagt acctgaaccg gcgcgcggcg ctcgaccgcc acctgtccga ggtgaagctc
      961 atcaccgacg gacagagcat cttcaaggtg gcccgccgga gcggggaggt cctggacgcg
     1021 ctgaaggagg gtcagctcgc cttcttcgtg gccatcgacg agatcgcgac gggcgtggag
     1081 gcgcgggtgg ggcagttccg tgaggaccgc gatcggttcg tccgggccct gcgcgaggcg
     1141 gccgctcacg cccagaccgg tcggtagctc acccggcggg ggctcgggcg gccgggggtc
     1201 gctcgcgccg gaggatcaca ggcccggccg gtagaccccg gcgatccggc gcgacaggct
     1261 cgcgtgtcgc cggaggtcct ggatcgctac ccgggcgtcg cgccgggcgg ctcgggcgtc
     1321 cgacgcgctc accgatcgct ccgagtacgc ggcggtggat gccatgctcg tgagccggcc
     1381 caggtgcccg tccgagaagc ggacgctccc agccagtcgc cgtcggtgct cggcgagcgt
     1441 ctccccctgt ctccgcccca gccccaggtc ggtggcctcg ccgtcgaaca cccggtacgc
     1501 cgccagcacc gcatgtcggg gatcgtggat ccggcggagc aaggaacggc gccagagctt
     1561 cttggccagg gggaacagca gggcgagcat cccggcgagg gccaggacca cccacaccac
     1621 cctccggtag gggctggggc gaccggcatc ggcgggctgc agggccggga tcgcgtccgg
     1681 gccgaggccc cgcgggcgcc gggccgtccc gtacgggttg gtgcagagct tggaggtgag
     1741 gccggggccg caatccgagg tggtggtggt ccggccgcct tgcgtctgcg tctcaccacc
     1801 cccccggttc ggtccgctgc tcgacgtgtc gggcgagagg taggagcctt gggggggcgt
     1861 cggcttgctc gtgccgggcg tggggtcgaa gggcagccat ccgtagcccg ggaagaggac
     1921 ctccacccag ctgtggctct gcttggtggt cacggtgaac gtggcgtcca actgcttgcc
     1981 gggcaggtag ccgacgacga cacgggcggg gatcccgagc tcgcgcacca gcgtggccat
     2041 ggccgtggcg aactgctggc agaaccctcg atgggtcttc gttaggaagt cgaccagagc
     2101 atcggcatcg gctcggggct tcacgtcgag cgagtactgg aacggcgagg agagcagccg
     2161 ctgctcgatc gtgtagacct gctcgtacgg ggttgccgcc tgcgccgtcc accggcgggc
     2221 gatctgcgcg acctcgtccg gaaccgaatc gggcaccttg gtgtcctgca gataggcggc
     2281 ggggtccgcg aaggagacct ggtccagctg ctggggcgtc gggaacagcc gtcccgacgt
     2341 caccgtgtat tccagaccag cgttgaggtc ccggccggcc accgcggaga tcaggttctg
     2401 gtcgtagtgg atcggcccga tcggcaggtc gatcgtccgc ggcggatagg ccatcggcag
     2461 atacggctgc gggatgtcgg tcaggaacct gtaggtctga tgcaccgggg gcaggccgga
     2521 gggcaggagg gaggggaagg tggccggcga gctcatcacc cttccccctg cgcccagcgg
     2581 atccgagctg ctccaggtca cgccgttgaa ccggtccagc gcgtacaggc gccagtaggc
     2641 ggccacgggg gtctcctgat cgtccaccga ggtgaccgtg aacaggttga tcggcgtccg
     2701 ttcctggagc tgggccttga tcgacacgaa ggggtcgagc ccgatgcggt cggggttcgt
     2761 cgtggagaag tccaccaggg ccgaggaccg gaaaccgggc aggatgccgg ggagcagcac
     2821 ggcgacggcc acggccacca gggccacctt ccgcgccccc gccgtggccg atcgggtgac
     2881 cgcgcgctcc cgcccgtgcc aggtccagat cggac
//
LOCUS       DASQQQ010000364        21287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_327698, whole genome shotgun sequence.
ACCESSION   DASQQQ010000364 DASQQQ010000000
VERSION     DASQQQ010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 21287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21287
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327698"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /gene="pgl"
                     /locus_tag="VE646_13605"
     CDS             complement(<1..475)
                     /gene="pgl"
                     /locus_tag="VE646_13605"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HZD81059.1"
                     /translation="MTGARELLVDRDWVSVAERLFLDAAPKTLALAGGSTPRALYERL
                     ARARFPWNGVDVFFGDERCVPPTDPDSNLHMAHEALLSRISARVHPMAGCDPAAYEHE
                     LEQVLGTGVPSLDLVFLGLGEDGHTASLFPGDRALEERERTVVLVDRPDHRRMTLT"
     gene            complement(472..909)
                     /locus_tag="VE646_13610"
     CDS             complement(472..909)
                     /locus_tag="VE646_13610"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZD81060.1"
                     /translation="MTDPDLMRMLLEAHRALAAELRDVLEERGYPQARAGYAAVFMHI
                     DRRSGTRLTELARRAHISKQGMMLIVDDLEQIGLVRRVRDPADGRAKVVRLTAKGRRF
                     VAEARRAMGTIEGRVRRELGDRRYEVLRSGLAQVVDERGPARP"
     gene            983..1474
                     /locus_tag="VE646_13615"
     CDS             983..1474
                     /locus_tag="VE646_13615"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZD81061.1"
                     /translation="MIEVRPVRPSEQEEAGRVTALAYREFARPGDPDWDEYLVRIADV
                     PERNGRTLVLVAVEEGDLLGSVTLELDGRTEPGGEPLAPHEAHIRMLGVHPDHRRRGV
                     ARELIHACFDRARAAGKTRVTLNTTTRMEAAQRMYETLGFRRTADRVFPDGFVLLGFE
                     RPL"
     gene            1648..2217
                     /locus_tag="VE646_13620"
     CDS             1648..2217
                     /locus_tag="VE646_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HZD81062.1"
                     /translation="MARTDLSGKRIACLATHGVEQVELTEPRKALEEAGAHVDLVSME
                     LGQIQGVDGMDKADVLHVDRAVRDVSVDDYDALLIPGGVANPDKMRTNDDAVGLVRSF
                     VGSGKPVAAICHGPWMLVEADVLRDRVITSWPSLETDVRNAGATWVNEEVHVDEGIVT
                     SRKPDDIPAFNAKMIEAFAQGTHQQRRAS"
     gene            complement(2254..4239)
                     /locus_tag="VE646_13625"
     CDS             complement(2254..4239)
                     /locus_tag="VE646_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD81063.1"
                     /translation="MRGHGRFLRPIPEEDKRPIRRSTVRRVAATFRPYRSKVAWVAVA
                     IAVTSGLGVVNPVLIKVIFDKALFGNPPGSCGGQPCPQLHTLYVLVGFMVAIPVISGA
                     IGIGQTYLANLVGLRVMQDLRNALYSHLQFMPLRFFTTTRTGEIQSRLSNDVGGVQSV
                     VTDTASSILSNSVVIISTLIAMLLISWQLTALSLSLLPFFLWLTYKVGKARQEVAVST
                     QKTLADLTAITEETLSVSGILLSKAFGRQRHEIGRFRGENERLTGLQIRQTMIGRSFF
                     AVVGTFFSITPALVYLVAGWVLTGNGTGGLTAGSIVAFTTLQSRLFFPIGSMLQVSTE
                     VQSSFALFDRIFEYLDMHHEIADAPDAVPLPSDAVTGRVSFDQVWFRYEAPTQEVPDP
                     FDPTWPVTGPPRPREWALEDVSLQIEPGQLAALVGPSGAGKTTMTYLVPRFYDVQRGE
                     VRIDGIDVRGLRLESLGELIGMVTQETYLFHTTIRRNLLYGKPDATHEELEAAARAAF
                     IHDRIAELPQGYDTIVGERGYKLSGGEKQRLAIARVILKDPRILILDEATSSLDTTSE
                     RLVQQALRPLMRGRTTIAIAHRLSTILSADVIFVVDRGRVVERGTHERLIEHGGLYAR
                     LYEQQFRGGEVETLTGEDVVLSGGGSVRSADDPGDAA"
     gene            complement(4295..4981)
                     /locus_tag="VE646_13630"
     CDS             complement(4295..4981)
                     /locus_tag="VE646_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZD81064.1"
                     /translation="MSKRELERIRDEVVECRRCPRLVAWRERAARVKVARFAGQEYWG
                     RPVPGFGDAGARVLIVGLAPAAHGGNRTGRIFTGDRSGDFLFSALHRTGFANQATSVA
                     LDDGLRLRNLYVTAVNRCAPPGNRPTPEERDNCLPYLAREVQALTGVGVIVALGAYAW
                     DGTLRALAGLGHVERPKPRFAHGAEASVGPNILLGAFHPSQQNTFTGKLTPEMFDAVL
                     LRARELAGPD"
     gene            5098..6696
                     /locus_tag="VE646_13635"
     CDS             5098..6696
                     /locus_tag="VE646_13635"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZD81065.1"
                     /translation="MAEHPGIRRWLARTAMVATVLAVSVAGSPSLPRGDAVPETVLRS
                     VGVDLGSLVRPGPGLSVSTAAGTLRAAEPVRTARTRACSPIWFDGMAFTWTQRAAGSA
                     GDVQVRAATGSDGRFGAVQTLEPEGGPDPGTAEYVGTERGSQYLWTGGATCARFSLVV
                     PAGTEVGDLRVLFINSSGTADGPGSASPDVGPVAAAPPQAPPGMQAAQAMTRRPRIIT
                     REQWGANPKLMNCATWVAPFLKMGFVHHTAGSNAYSRAQADDVVRGIYAYHTNGRGWC
                     DIGYNFLVDRYGDVFEGRSGGVTNNVVGAAQMGFNTGAFSVSMMGNFDVARPPRRAVR
                     ALERLVAWRLDVAHVNPRARTVMTSAGGSTTKYAAGTEVRLRTISGHRDTGLTACPGR
                     YLYPLLPTVRSVVGRMGLPKIYGPRLSSGVLTSGVPGRLHIRAKSSGQYRWRVTIQAP
                     GGAPVARIRGARSRLLDARWWTDGTLAAGAYRVLVETTGAVGRRARPVVLPLTVEPEP
                     EPSPSPSPSPSPSPSPSPTVSPTP"
     gene            complement(6718..7119)
                     /locus_tag="VE646_13640"
     CDS             complement(6718..7119)
                     /locus_tag="VE646_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81066.1"
                     /translation="MILRRKRLPPELKERFEGFRAAVEPLEEAKAALPPAVPTTRLPG
                     RPLAEVLLEYEVGLDRTAERMNAWRAPEVQGEWDACWAGLREARAMAERLRTQAPDLG
                     GFEGLIGVLGDLLAPLEAFEIAAERFRSLRR"
     gene            complement(7116..7898)
                     /locus_tag="VE646_13645"
     CDS             complement(7116..7898)
                     /locus_tag="VE646_13645"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HZD81067.1"
                     /translation="MADRSWQDAGLPVVVAHRGASSTHPENTRSAFEAAIALGARLVE
                     TDVRLTADGEPVLIHDPEVSRTTDGSGWVHQLATTELRSMNAGTQVRPEPVPTLREGL
                     ELVSGRAGIALEIKNLPGEPAFEAGGEAIVEAAAATLEAVRFPGPVLVLSFNPRSIGE
                     ARRLLPGAATGFLITDATAPDAGLAHVVDAGHDLLLPGRRGLAGAGPGFVKRAHGQGV
                     RVGTWTVDDPEDVRRFLDWGVDAVASNDPAMALGVLEAWRAG"
     gene            complement(7925..8599)
                     /gene="nth"
                     /locus_tag="VE646_13650"
     CDS             complement(7925..8599)
                     /gene="nth"
                     /locus_tag="VE646_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HZD81068.1"
                     /translation="MPARSTSSKGPDRNDPAEQRAPAIVRRLSRAYPDAKVALRFSTP
                     LEMLVATILSAQCTDERVNQVTETLFRKYRTPRDYLKVSESELATDIKPTGFFNQKTR
                     SIRGACRRIVDAYEGEVPDTMDDLVSLPGVARKTANIVLGNAFGRVEGIAVDTHVRRV
                     AQRLGFTEQQDPDKIEQDLMRLIPRPRWFDFTYVLIDHGRATCQARRPRCEDCPVNQL
                     CPASLV"
     gene            complement(8629..9378)
                     /locus_tag="VE646_13655"
     CDS             complement(8629..9378)
                     /locus_tag="VE646_13655"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HZD81069.1"
                     /translation="MSALVLGLLGSGEFEPWAEQVDRWLLERATGDGKVLILPTASAP
                     EGDEVFDFWAQRGRAHYRGMGVEAEVVPIKTREDAGRPEFAAKLAGASEVFFSGGNPA
                     SLAQILSGTHFHRELVHAMRRGLAYGGCSAGVACLPELSPDSSLQTLAEIRRDGWKPG
                     LQLFSGVLFMPHWDALDGFLPGLTDWVVSSVPAGCRLLTIDENTALVGDGRGWSVLGL
                     GGVRVLTDGSWEEHPSGGSFELSLALGAGMA"
     gene            complement(9375..10889)
                     /locus_tag="VE646_13660"
     CDS             complement(9375..10889)
                     /locus_tag="VE646_13660"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HZD81070.1"
                     /translation="MTVDPLEPFGDLDWDPERARAFTDRTAGLFEELLRRLPELPVTR
                     GRSADQVRDAVAIPIPEDPMPDDALFDHLREMTFEHSTYPGHPRFLAYITGAGTVPGA
                     AADLLAAGLNMNLGGWLLSPAATEIELALTRWFARRFGLPEEAGGLIVSGGAMANFVA
                     LKVARDRRAGWDVRTEGVRTGPPLAIYLSEETHVVSTRAADLLGIGTAGVRAIPVDEG
                     YRIRMDLLRAAIGRDRDEGIRPVAVVGSAGTVATGAVDPLDALADLCAEEGIWFHVDG
                     AYGGPAAFAPDLRPLLSGIERADSIAFDPHKWLYTPHSGGCVLVRDLQHLADSFAVHA
                     TYVQQDKERTGRGLDFTQLGPQFSRGFWALKIWLSLLAHGARAYRERISHDAALARYL
                     GERVRERPGFELVCPVGLSICCFRYVPSDLPEGPDREAYLDLLNERLMTEIQQDGRVF
                     ISNAVLGERFVLRVCIVNFRTESEDLDAVLEVTEELGARLDAELRPTSLRGVEV"
     gene            complement(10886..11596)
                     /locus_tag="VE646_13665"
     CDS             complement(10886..11596)
                     /locus_tag="VE646_13665"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZD81071.1"
                     /translation="MDELGTVLGVWAHPDDETYLMAGLAARAVRNGSRVVIVTATRGE
                     AGSMDDERWPPETMGDLREQELIRCLAILGVSEHHWLGLPDIDMQTALPEEGYEKVRS
                     LMAEIQPDTVMTFGPDGMTDHEAHKSVCEWSTRAFHEVGKPGARLYYATVTPEWGAEF
                     LPLYEPFNVFLPGTPPITPPEELGIDYRLPPDILDIKMRAIDQHESQVGAMREAFGED
                     IFSRSMNAEWFRLAAVKA"
     gene            complement(11728..11919)
                     /locus_tag="VE646_13670"
     CDS             complement(11728..11919)
                     /locus_tag="VE646_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81072.1"
                     /translation="MMKGTRPGHLPAPSKTWDEEGRVCRVPGCGTRLSMYNAGETCWQ
                     HATITFPNFRGKRLVDPRA"
     gene            complement(12059..12313)
                     /gene="rsrA"
                     /locus_tag="VE646_13675"
     CDS             complement(12059..12313)
                     /gene="rsrA"
                     /locus_tag="VE646_13675"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HZD81073.1"
                     /translation="MSSIDCQDVLKQIELYLDGELDPSLRSQVSDHLGRCSPCMSHSE
                     FTRHLRELLRAKCGCGEVPDHVMDRVREVLFELPGGPAPA"
     gene            complement(12300..12887)
                     /locus_tag="VE646_13680"
     CDS             complement(12300..12887)
                     /locus_tag="VE646_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011211119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZD81074.1"
                     /translation="MARSRPTPIDDPVMRERFERDVLPLLPNLYGAALRLTRNPSDAE
                     DLVQESYLRAYRGFGGFQEGTNLRAWMYRILTNTFINSYRKRQRQPQTVSDEDIEDWY
                     LYDKLGESGAQSSAESEVLDALPDEDVQRALEALPEGFRMAVLLADVEGFSYKEIAEI
                     LDIPIGTVMSRLHRGRKALEKALWETVRERGLVVD"
     gene            12990..15017
                     /gene="ftsH"
                     /locus_tag="VE646_13685"
     CDS             12990..15017
                     /gene="ftsH"
                     /locus_tag="VE646_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZD81075.1"
                     /translation="MAGGDNDRRGRWTGFDFGGGSPGEPRRFRASAWIWIIGFLLLLF
                     LINYLAAPRPTNIDYSVFLQKVQQGQIVGTVQISQTSVSGTYQEGGKNVDFTATLPPI
                     LQGTTQLTETLQQNDIRYTGVQPSGWSSLLVGWVVPLLLFAALWIFIIRRMGGAQTSA
                     ALNLGRNRVKIYDRKEMKTTFADVAGVDEAVEELREVVDFLKHPKKYQRLGGRIPKGV
                     LLLGPPGCGKTLLARAVAGEANVPFFFMSGSEFVEMFVGLGAARVRELFQQAKEKAPA
                     LVFIDELDTIGKGRGGALGAGFGAHDEREQTLNQLLVEMDGFDSSKGVIIMAATNRPD
                     VLDPALTRPGRFDRQVVVDRPDLRGREAILRVHARGVAMDPGVDLRLLAARTPGFTGA
                     DLANVVNEAALLAARAEKNTVTMSELEEAIDRVTAGLERKSRVMSEREREIVATHEMG
                     HALVALNMPTADPVHRVSIIPRGAAALGMTMQRPLEDRYLLTEPELKDRLAVLLGGRT
                     AEELGFQQISTGAQNDLQRATEISRAMVTEYGMSDRVGPLSFGNDGFRSPEGRMLFPG
                     AAPELSPDLAALVDEEVKRLVDEAHTRARTALEEHRDLLDRLADLLMVTEVIEGPDLM
                     AYVDGSRPIPTLEEARERIAAGNGDSKTNGSSGKARPVSMPPPPVPVDPSS"
     gene            complement(15076..16698)
                     /locus_tag="VE646_13690"
     CDS             complement(15076..16698)
                     /locus_tag="VE646_13690"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZD81076.1"
                     /translation="MSDLVIALTVLAWLAYLAGRLLRRTLPELIVFLGAGALMGPEVL
                     GLLDAQDLGRLEGVIQLALAVLMFTVGERVSVRALRRARWVGLAGLVQYLICAALVFA
                     VARWVGATNNVAVLLAALAGAGAPMTVASVVASTRGRSPYGEALIGVHAVGDALAAVA
                     FAAALPIVEELLHPGTSLASAGIRFGWVALGTVALGSFFGWVVARFGPGIEGSGELLL
                     FILVHVLVVASASAALGLSLPLAALVMGAVAASLGPSDAAQRTFVAVRQVEQPLYLLF
                     FALAGASIHFSALPALGGVGLAYLLARTAGKVAGGWLVGIAGNIPGRSSLRLGMDLMP
                     QAGVAVGLAVLAAESLPGTGGHVAAVVLGSVVLFELVGPLLVARGLRSEPPMPTVPED
                     GVGPIDDRLPTSILLASEVRTTAPAWLVDWFARTGASLTVLRHPDEEGTGELRAVEDL
                     ARDRGIPVRSASLSGETFAGSVIRTAREVDADLVVLVVLPRAPDRLAMRLTLLPQERI
                     ARHLSCPIVLVPVEEPVGEPSGRLSKLLRGLP"
     gene            complement(16730..17668)
                     /locus_tag="VE646_13695"
     CDS             complement(16730..17668)
                     /locus_tag="VE646_13695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZD81077.1"
                     /translation="MPESEVPREVRELAAEREERRQARDFGPADALRERISELGYRVI
                     DGPAGPSFEPLGPAAGGGEQARGASSRAIPSLLDEPAIADASVHWVLQGWPEDVRRAL
                     AGFRRFAGPRTIQYVVADVTGAAPSTFGADVEVVSLEEGTGWAAARNAGLRRSLGRIV
                     LAMDGSVEPEGDVLGPLENALVDPDTGVCGPFGVVTDDLRDFEASSGPEVDAIEGYLM
                     AFRRELLTEVGGFDERFRWYRTADIEYSFRVRDTGLRARVVPVPVRRHEHRAWASTPP
                     EERARLSKRNYNRFLDHFRGRFDLLVRGPGSRPAGG"
     gene            complement(17661..18431)
                     /locus_tag="VE646_13700"
     CDS             complement(17661..18431)
                     /locus_tag="VE646_13700"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZD81078.1"
                     /translation="MARLERPDGCGLYYEVHGDPRRSPLVLLEGLGGDVAGWASSLPL
                     LAAELFVVAYDLRGNGGSDPPTGPLEMGTFIEDTVALLDELGVERAHVLGISLGGMVA
                     QELALGHPERVRALVLGATHAGGHHVIPSAYRAPKGDPSLALVSEGFAAEHPQQLRER
                     WRGRRRAGVPGAARRQREAIRGFDTYDRLPRITAPTLVIHGTGDRMIDSRNAEVLASR
                     IAGAELVLLKGAGHVFHWEQPERVDMVVLDFLRRHRDA"
     gene            complement(18445..18885)
                     /locus_tag="VE646_13705"
     CDS             complement(18445..18885)
                     /locus_tag="VE646_13705"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZD81079.1"
                     /translation="MGRREVEAVAKDVEERIAQVPIFSNCTQKELALVARAAKEVTHR
                     SGTIIAREGQRGLGLFVILEGRCSVTIGGMRRATLGAGDFFGEIALLDGGPRSATVTA
                     ETDVRLAGLTEWVFRGLLQEHPSIAIKTLESVAGRLRTVAKEQV"
     gene            complement(18954..19859)
                     /locus_tag="VE646_13710"
     CDS             complement(18954..19859)
                     /locus_tag="VE646_13710"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HZD81080.1"
                     /translation="MDRVVELIVGGATVPERLASTAIAIAAALLLGWFSGWLLGKRVD
                     DPYARYYARKLSHYVVAVAALVAIGVIWRAFAGRVGLVLGVVAAGLAFAMQEVIGAVA
                     GWFNILSGRIFAVGDRIEMGGVRGDVIDVTPLRTKILEMGSPVEGASWVQGRQYTGRI
                     VSVSNKATFTEPVYNYSAVFDFIWEELVVPIPYDADWRRAESILREEAERISASKEAR
                     EAMSTMVRRYPVPRAEVEPRVFARATDNWMELAARFVVPVRTARSTKDRMVRRIRDRL
                     DRADIRIASETLEATVRGQGSGTDE"
     gene            19972..20736
                     /locus_tag="VE646_13715"
     CDS             19972..20736
                     /locus_tag="VE646_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009192732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HZD81081.1"
                     /translation="MEIYHHPPAWLEALSWGSLAVAFATAAVILADIYGRGHRQKMPI
                     MEAVYPISALYWGPVALWFYLRFGRRKSKKIMAERASSPGPGAMAMEERPEQGERSSE
                     DQEDRVRWWQVAKADSHCGAGCTLGDIGAEWLVFTLGLLIAGKALYADFPLDLAFAWT
                     LGVVFQYFTIVPMRDLGPLRGIWAAIRADTLSIVAFQIGLFAGMFVYQELIFPNPLPK
                     TSATYWMFMQLSMILGFFTALPANRWLLRKGWKEKM"
     gene            complement(20678..>21287)
                     /locus_tag="VE646_13720"
     CDS             complement(20678..>21287)
                     /locus_tag="VE646_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81082.1"
                     /translation="PMSLGCLMAGGGLFVVNALLQPSVSLVSLTWALAVVGFGFGMAL
                     VTMNATVLSVVPPQRSGMAASTVNTSRELGGVFGVAILGAIMSATITSHMAQRLRELG
                     IPPSFREIVIQGITHGAVPTSASGAPGAPAGTGALVEKVIQAAQTAFTNGLGQVLILA
                     GSILVAAGVAAAFMARRPQPPRTPTSSPSSPSGAASGSRAGR"
BASE COUNT         3256 a         7792 c         7312 g         2927 t
ORIGIN      
        1 gggtcagcgt catccgccgg tgatcgggcc ggtccaccag caccaccgtc cgctcccgct
       61 cctccagcgc ccgatcgccg gggaacagcg acgccgtgtg accgtcctcg cccagcccca
      121 ggaacaccag gtccagcgag gggacgccgg tgccgaggac ctgttcgagc tcgtgctcgt
      181 aggcggccgg gtcgcagccg gccatgggat gcacgcgggc ggagatccga gacagcagcg
      241 cctcgtgggc catgtggagg ttggagtcgg ggtcggtggg gggcacgcat cgctcgtcgc
      301 cgaagaagac gtcgacgccg ttccagggga accgcgcacg cgccaggcgc tcgtagaggg
      361 ctcgaggggt cgagccgccc gccagggcca gtgtcttcgg cgccgcgtcc aggaagagcc
      421 gctccgcgac cgagacccaa tcccggtcca ccaggagctc gcgagccccg gtcacggccg
      481 ggccggcccc cgctcgtcca ccacctgcgc caggccgctc cgcaggacct cgtaccggcg
      541 gtcgccgagc tcgcgccgca cccggccctc gatcgtgccc atcgcgcgac gggcctcggc
      601 cacgaaccgc cgccccttgg cggtgagccg caccaccttg gcccggccgt ccgcgggatc
      661 gcgcacccgg cgcaccaacc cgatctgctc caggtcgtcc acgatgagca tcatcccttg
      721 cttggagatg tgagcccggc gggcgagctc ggtcagccgg gtgccggagc ggcggtcgat
      781 gtgcatgaac accgccgcgt acccggcccg ggcctgcggg tagccgcgtt cctccagcac
      841 gtcccggagt tcggcggcaa gcgctcggtg cgcttccagg agcatccgca tcagatctgg
      901 gtcggtcacg ggacctccgg ctcgaggcgt tcggcgcggc ccccggtcgt cgggtcgcgc
      961 ctgccgcgat agcctgccac gcatgatcga ggtccgtccc gtccggcctt cggaacagga
     1021 ggaggcgggg cgggtcaccg ccctcgccta ccgggagttc gcgcggcccg gagaccccga
     1081 ctgggacgag tacctggtcc gcatcgccga cgtgcccgag cgcaacggcc gaaccctggt
     1141 cctggtggcc gtggaggagg gcgacctgct cggctcggta acgctcgagc tggacggccg
     1201 gaccgagccg ggcggggagc cactcgcccc ccacgaggcc cacatccgga tgctgggcgt
     1261 ccatccggac caccgccggc gcggcgtagc cagagagctg atccacgcgt gcttcgaccg
     1321 ggcccgggcg gcgggcaaga cccgcgtcac cctgaatacc acgacccgga tggaggcggc
     1381 gcagcgcatg tacgagacgc tcggcttccg gcgcacggcc gaccgcgtgt tccccgacgg
     1441 gttcgtcctg ctcgggttcg agcggccgct ctgagggctt gcccgcggcc gggcccatcc
     1501 ggtttccgcc tcggaggcgg ggggtaggcg cagttcaggg ggacggtgca cggcgatggt
     1561 tcgtgcccgg aagcgaccga cggccaatct cgtcccggct ccggcatggc cgcgagtccg
     1621 ggccgatacc gataggaggt gctcgacatg gcccgcacgg acctgagcgg caagcgcata
     1681 gcctgcctgg ccacacacgg cgtggagcag gtggagctga ccgagccccg gaaggcgctg
     1741 gaggaggccg gcgcgcacgt ggatctcgtg tcgatggagc tcggccagat ccagggtgtg
     1801 gacggcatgg acaaggccga cgtcctccac gtggaccgcg cggtgcgcga cgtgagcgtg
     1861 gacgactacg acgcgctcct gatccccggc ggcgtggcga acccggacaa gatgcgcacc
     1921 aacgacgacg ccgttggcct ggtccgctcc ttcgtgggat cgggcaagcc cgtggcggcg
     1981 atctgtcacg gcccgtggat gctggtggag gccgacgtcc ttcgtgatcg cgtgatcacc
     2041 tcgtggccga gcttggagac cgacgtccgc aacgccggcg ccacctgggt gaacgaggag
     2101 gtgcacgtcg acgagggcat cgtcaccagc cgcaagcccg acgacatccc ggccttcaac
     2161 gccaagatga tcgaagcgtt cgcccagggc acacaccagc agcgccgagc ctcgtaacgc
     2221 atcgagcgga gccggtccgg tcggcgccgc cgctcaggcg gcgtcgcccg gatcgtccgc
     2281 cgaacgcacg gacccgcctc ccgagagcac cacgtcctcc ccggtcaggg tctccacctc
     2341 gccgccccgg aactgctgct cgtacagcct cgcgtacagg ccgccatgct cgatcagccg
     2401 ttcgtgggtg ccgcgctcga cgacgcgtcc ccggtccacc acgaagatca cgtcggctga
     2461 caggatcgtc gacagccggt gggcgatggc gatggtggtc cggccccgca tcagcggccg
     2521 cagggcttgc tgcacgagcc gctcggaggt ggtgtccaag ctcgacgtgg cctcgtccag
     2581 gatcaggatc ctggggtcct tcaggatcac gcgagcgatg gccagccgct gcttctcccc
     2641 gcccgagagc ttgtacccgc gctcccccac gatcgtgtcg tagccctggg gcagctcggc
     2701 gatgcggtcg tggatgaagg cggcccgggc cgcggcctcg agctcctcgt gggtggcatc
     2761 gggcttgccg tacagcaggt tccgccggat cgtggtgtgg aacaggtacg tctcctgcgt
     2821 gaccatcccg atcagttcgc cgagcgactc gagccgcaac ccccgcacgt cgatgccgtc
     2881 gatccgcacc tcgccacgtt gcacgtcgta gaagcggggg accaggtagg tcatcgtggt
     2941 cttgccggcg ccgctcggtc ccaccagggc tgccagctgc ccgggctcga tctgcagcga
     3001 gacgtcctcc agggcccact cgcgcgggcg cggcggcccg gtcacgggcc aggtcgggtc
     3061 gaagggatcg ggcacctcct gcgtgggtgc ctcgtaccgg aaccagacct gatcgaagct
     3121 gacccgcccg gttacggcgt cggaggggag cggcacggcg tccggcgcgt cggcgatctc
     3181 gtggtgcatg tccaggtact cgaagatccg atcgaacagg gcgaaggagg actggacctc
     3241 cgtggatacc tgcagcatcg acccgatcgg gaagaacagc cgcgactgca gcgtggtgaa
     3301 cgcgacgatc gaccccgccg tcaggccgcc ggttccgttc ccggtcagaa cccacccggc
     3361 caccaggtag accagcgccg gcgtgatcga gaagaaggtc ccgaccaccg cgaagaagga
     3421 ccgaccgatc atcgtctgcc tgatctgcag accggtaagc cgctcgttct cgccccggaa
     3481 ccggccgatc tcgtgccgct gtcgcccgaa cgccttcgac agcaggatcc ccgagaccga
     3541 gagcgtctcc tccgtgatcg cggtgaggtc cgccagggtc ttctgcgtgg acacggccac
     3601 ctcctgccgc gccttgccca ccttgtaggt cagccacagg aagaagggca gcagggacag
     3661 cgagagggcc gtgagctgcc agctgatcag cagcatcgcg atgagcgtgc tgatgatgac
     3721 caccgagttc gagaggatcg aggaggcggt gtcggtcacc accgactgga cgccgcccac
     3781 gtcgttcgac agtcgggact ggatctcgcc ggtccgggtg gtggtgaaga accgcagggg
     3841 catgaactgc aggtgcgagt acagggcgtt gcgcaggtcc tgcatcacgc gaaggcccac
     3901 caggttcgcc aggtacgtct gcccgatccc gatcgcgcct gagatcaccg ggatagccac
     3961 catgaacccg acgagcacgt acagggtgtg cagctgcgga cagggctggc cgccgcatga
     4021 tccgggggga ttcccgaaca gggccttgtc gaagatcacc ttgatcagca ccgggttcac
     4081 cacgccgagg cccgaggtca ccgcgatggc cacagccacc caggccacct tgctccggta
     4141 cgggcggaag gtggctgcca cccggcggac ggtgctccgc cggatgggcc gtttgtcctc
     4201 ctccgggatg ggacgaagga acctgccgtg gccacgcatg cccctcaggg taaccccatg
     4261 ggccgacccg gagaccctcc gtggcgaacg agcctcagtc cggaccggcg agctccctgg
     4321 ctcgcagcag caccgcgtcg aacatctccg gcgtgagctt gccggtgaag gtgttctgct
     4381 ggctcgggtg gaaggcgccg agcaggatgt tgggaccgac cgatgcctcc gccccgtgag
     4441 cgaaccgggg cttgggccgc tcgacgtggc ccagcccggc gagcgctcgg agagtcccgt
     4501 cccatgcgta cgcgcccagc gcgacgatca ccccgacccc ggtcaaggcc tgcacttctc
     4561 gggccaggta gggcagacag ttgtcccgct cctcaggcgt gggcctgttg cccggaggcg
     4621 cgcaccggtt cacggccgtc acgtagaggt tccggagcct gagcccatca tccaacgcca
     4681 ccgaggtcgc ctggttcgcg aaccccgtcc ggtgcagggc cgagaacagg aagtcacccg
     4741 agcggtcgcc cgtgaagatc cggccggtgc ggttcccgcc gtgcgcggcc ggggccagcc
     4801 ccacgatcag cacccgtgcc ccggcgtcgc caaaccccgg caccggccga ccccagtact
     4861 cctgaccggc gaagcgggcc accttcaccc gggctgcccg ttcccgccac gccacgagcc
     4921 tggggcagcg tcgacactcc acgacctcgt cgcggatcct ctccagctct cgcttgctca
     4981 cgccgttccc gtccccccgc cggccgacgg accgccgagg atgctacttc ccccgatcgg
     5041 gtggttgcct ggccgggcgg gaaccggtag acagaccagg cgacgacgcc cgaacccatg
     5101 gccgaacatc ccgggatccg ccgttggttg gcccgcaccg cgatggtggc gacggtcctg
     5161 gccgtctcgg tggccggatc gcccagcctg ccccgagggg acgcggtgcc cgagaccgtc
     5221 ctgcgctcgg tgggggttga cctcggaagc ctggtccgcc ccggccccgg cctcagcgtg
     5281 agcacggcgg ccgggaccct tcgagccgcc gagccggtgc gcacggcccg gactcgagcg
     5341 tgctcgccca tctggttcga cgggatggct ttcacgtgga cgcagcgcgc ggccggttcg
     5401 gccggtgacg tgcaggtccg ggcggcgact ggatcggacg gacgcttcgg ggctgtgcag
     5461 accctggagc ccgagggcgg gcccgacccc ggcacggccg agtacgtggg gaccgagcgc
     5521 ggctcccagt acctgtggac cggcggcgcg acctgcgctc gcttctcgct ggtggtcccg
     5581 gccgggaccg aggtgggcga cctccgggtg ctgttcatca acagctcggg cacggccgat
     5641 gggccgggga gcgcctcccc ggacgtgggg ccggtggccg ccgctccgcc gcaggcgccg
     5701 ccgggcatgc aggcggcgca ggccatgacc cgtcgccccc ggatcatcac ccgggagcag
     5761 tggggggcga accccaagct catgaactgt gccacatggg tggcgccgtt cctgaagatg
     5821 gggttcgtgc accacacggc cgggtccaac gcctactccc gagcccaggc ggacgacgtc
     5881 gtgcgcggga tctacgccta ccacacgaac ggccggggct ggtgcgatat cggctacaac
     5941 ttcctggtcg accggtacgg ggatgtgttc gaagggcggt ccggcggcgt gacgaacaac
     6001 gtggtgggcg ccgcgcagat gggcttcaat accggggcgt tctcggtgtc gatgatgggg
     6061 aacttcgacg tggcccgacc accgaggcgc gcggtccggg ccctggagcg gctcgtggct
     6121 tggcggctgg acgtggcgca cgtcaacccg agggcccgca ccgtgatgac ctcggccggc
     6181 ggcagcacta cgaagtacgc agcggggacc gaggtccgcc tgcggaccat ctcggggcac
     6241 cgcgataccg gcctcaccgc ctgcccgggc cgctacctgt acccgctcct gcccacggtc
     6301 cgctcggtgg tgggtcgcat ggggctgccg aagatctacg gaccacgtct ctcgagcggc
     6361 gtgctgacct ccggcgttcc cggccgcctc cacatccgag cgaagagctc cggccagtac
     6421 aggtggcggg tcacgatcca ggcgcccggt ggagccccgg tggctcggat ccgcggtgcg
     6481 cggagccgtc tcctcgacgc gcgatggtgg accgacggca ccctggcggc gggggcctac
     6541 cgggtcctgg tggagacgac cggggcggtc ggccgacggg ctcgcccggt tgtgctcccg
     6601 ctgaccgtgg agccggagcc ggagccgagc cccagcccca gcccgagccc cagccccagc
     6661 cccagcccca gcccgacggt ttcgcccacg ccctgaccct cctcggcggg agggtcctca
     6721 gcgccgcaag ctgcggaacc gctcggccgc gatctcgaag gcctccaacg gcgccagcag
     6781 gtcgcccagc acgccgatga gcccctcgaa cccgccgagg tccggcgcct gggtccgcag
     6841 acgctcggcc atggcccggg cctctcggag cccggcccag caggcgtccc actccccctg
     6901 cacctcgggc gcccgccagg cgttcatccg ttccgcggtt cggtccaacc ccacctcgta
     6961 ctcgagcagc acctcggcga gaggccggcc cggcagccgg gtggtgggca ccgcgggggg
     7021 cagcgccgcc ttggcctctt ccaggggctc caccgccgcc cggaaccctt cgaaccgctc
     7081 cttcagctcc gggggcaggc gcttgcgacg aaggatcatc cggccctcca ggcctccagc
     7141 acgccgaggg ccatggcggg atcgttcgag gccaccgcgt ccacgcccca atcgaggaag
     7201 cggcggacgt cctcgggatc gtccacggtc caggtcccca cccgcacccc ctgtccgtgg
     7261 gcgcgcttga cgaacccggg ccccgccccg gccaggcctc gccggcccgg cagcaggagg
     7321 tcgtgtccag cgtcgaccac gtgcgccaag cccgcgtccg gggccgtggc gtccgttatc
     7381 aggaacccgg tcgcggcgcc gggcagcagg cgacgcgcct cgccgatcga ccgagggttg
     7441 aacgagagca ccaacaccgg ccccgggaag cgaaccgcct cgagcgtggc ggcggcggcc
     7501 tcgacgatgg cctcgccacc cgcttcgaag gcgggctccc cggggaggtt cttgatctcg
     7561 agggcgatcc cggctcgtcc actcacgagc tccaggccct cccgcagggt gggcacgggc
     7621 tcgggccgca cctgggtccc ggcgttcatc gaccgcagct cggtcgtggc gagctggtgg
     7681 acccagccgc tgccgtcggt ggtccgtgac acctccgggt cgtggatcag caccggctcg
     7741 ccgtcggcgg tcagccggac gtcggtctcc accaaccgtg ccccgagggc gatcgctgcc
     7801 tcgaacgccg agcgggtgtt ctccgggtgc gtggacgacg ctccccggtg ggccaccacc
     7861 acgggcaagc cggcatcctg ccacgagcga tcggccatgg acccgccgct ctccgccgga
     7921 cggctcacac gaggctcgcc ggacagagct ggttcaccgg gcagtcctcg caccgcggac
     7981 gccgggcctg acacgtggca cgcccgtgat cgatcaggac gtaggtgaag tcgaaccatc
     8041 gcgggcgggg gatcaggcgc atcaggtcct gctcgatctt gtccggatcc tgctgctccg
     8101 tgaagcccag gcgctgtgcg acgcgccgga cgtgggtgtc cacggcgatc ccctcgaccc
     8161 tcccgaacgc gttgccgagc acgatgttcg cggtcttgcg ggccaccccc ggcaggctca
     8221 ccaggtcgtc catcgtgtcc ggcacctccc cctcgtaggc atccacgatc cgtcggcacg
     8281 caccgcggat cgacctcgtc ttctggttga agaagcccgt gggcttgatg tcggtggcca
     8341 gctccgactc ggacaccttc aggtagtccc ggggagtgcg gtacttgcgg aagagcgtct
     8401 cggtgacctg gttcacgcgc tcgtcggtgc actgcgccga caggatcgtg gccaccagca
     8461 tctccagcgg cgtggagaag cgcagggcga ccttcgcatc cgggtaggcc cgggacaggc
     8521 ggcgaacgat cgcgggggcg cgctgctcgg cggggtcgtt tcggtcgggt cccttcgagg
     8581 aggtggatcg ggcaggcatc gggcggatgc tagcgcgctg gagggcccct aggccatgcc
     8641 cgctccgagg gcgagggaca gctcgaagga tccgccggag gggtgctcct cccacgatcc
     8701 gtcggtgagc acccggaccc cgccgaggcc caacacggac catccccggc cgtcccccac
     8761 gagggccgtg ttctcgtcga tcgtgagcag ccggcatccg gcggggaccg agctcaccac
     8821 ccagtccgtc agcccgggca ggaacccatc cagtgcgtcc cagtgaggca tgaagagcac
     8881 gccgctgaac agctggaggc cgggcttcca cccgtcccga cggatctccg ccagggtctg
     8941 caaggagctg tcgggcgaga gctccgggag gcaggccaca ccggcgctgc acccaccgta
     9001 cgcgagcccg cggcgcatcg cgtggaccag ctcgcggtgg aagtgcgtgc cggacaggat
     9061 ctgggcgagc gaggcggggt tcccccccga gaagaagacc tcggaggcgc cggcgagctt
     9121 ggccgcgaac tccgggcgcc ccgcatcctc ccgggtcttg atgggcacga cctccgcctc
     9181 cacccccatg ccacggtaat gggcccgtcc tctctgagcc cagaagtcga acacctcgtc
     9241 gccctcgggg gcgctggccg ttggcaggat cagcaccttg ccatccccgg tcgcccgctc
     9301 gagcagccac cggtcgacct gctccgccca cggctcgaac tcacccgatc cgaggagccc
     9361 caacacgaga gcgctcacac ttccaccccc ctcagcgacg tcggacgcag ctccgcgtcc
     9421 aagcgagcgc cgagctcctc ggtcacctcg agcaccgcat ccagatcctc cgactcggtc
     9481 cggaagttca cgatgcacac ccgcaggacg aaccgctcgc cgagcacggc gttcgagatg
     9541 aacacgcggc cgtcctgctg gatctccgtc atcagccgct cgttcaacag gtccaggtag
     9601 gcttcccggt ccgggccctc gggcagatcg gagggcacgt agcgaaagca gcagatcgag
     9661 agacccaccg ggcagaccaa ctcgaacccg ggccgctccc gcacccgctc tcccaggtac
     9721 cgggccagcg ccgcgtcgtg ggagatccgc tcgcggtagg cgcgagcgcc gtgtgcgagc
     9781 aacgacagcc agatcttcaa ggcccagaag ccgcgggaga actgaggccc gagctgcgtg
     9841 aagtccagcc ctcggccggt tcgctccttg tcctgctgga cgtaggtggc gtgcacggcg
     9901 aaggaatccg ccaggtgctg caggtcccgc accagcacgc agccgccgga gtgcggcgtg
     9961 tagagccact tgtgcgggtc gaaggcgatc gagtcggcgc gttcgatgcc cgacaggagc
    10021 ggccggaggt ccggggcgaa ggcggccggg cccccgtacg cgccgtccac atggaaccag
    10081 atcccctcct ccgcgcagag gtccgccagc gcatccaggg ggtccaccgc gcccgtggcc
    10141 accgtgccgg ccgagcccac cacggccacg gggcggatcc cctcgtcccg gtcccggccg
    10201 atcgccgctc gcaggaggtc catccggatc cggtacccct cgtccaccgg gatcgcccgg
    10261 acgcccgcgg tgccgatgcc gagcaggtcc gccgcccggg tgctgaccac atgggtctcc
    10321 tcggataggt agatcgccag cgggggcccc gtccgcaccc cctcggtccg cacgtcccac
    10381 ccggcccggc ggtcccgagc caccttcagg gccacgaagt tggccatggc gcccccggag
    10441 acgatcagcc ctccggcctc ctcgggcagc ccgaaccggc gagcgaacca ccgcgtcagc
    10501 gccaactcga tctccgtggc ggccggcgac aggagccaac cgcccaggtt catgttcagg
    10561 cctgccgcga gcaggtccgc tgcggcgccg ggcaccgtgc cggcacccgt gatgtaggcc
    10621 aggaaccggg gatgccccgg gtacgtcgag tgctcgaagg tcatctcccg caagtggtcg
    10681 aagagtgcat cgtccggcat ggggtcttcc gggatgggga tcgcgacggc gtcgcggacc
    10741 tggtcggcgc ttcgaccgcg cgtgacgggc agctccggca ggcgccgcag gagctcctcg
    10801 aacagcccgg cggtgcggtc ggtgaacgct cgcgcccgct ccggatccca gtccagatcc
    10861 ccgaacggct ccagcggatc gaccgtcatg ccttcacggc ggccagccgg aaccactccg
    10921 cgttcatcga cctggagaag atgtcctcgc cgaacgcctc ccgcatggcc cccacctggc
    10981 tctcgtgctg gtcgatggct cgcatcttga tatcgaggat gtccgggggg agccggtagt
    11041 cgatccccag ctcctcgggg ggcgtgatgg gcggcgtgcc cgggaggaag acgttgaacg
    11101 gctcgtagag ggggaggaac tccgcccccc actccggggt gacggtcgcg tagtagagcc
    11161 tggcccccgg cttgcccacc tcgtggaagg cgcgtgtgga ccactcgcac acggacttgt
    11221 gcgcctcgtg atcggtcatc ccgtccggcc cgaaggtcat gaccgtgtcc ggctggatct
    11281 ccgccatgag cgagcggacc ttctcgtagc cctcttcggg cagggccgtc tgcatgtcga
    11341 tgtccggcaa gccgagccag tggtgctcgg aaaccccgag gatcgccagg cagcggatca
    11401 gctcctgctc gcggaggtcg cccatggtct cgggcggcca ccgctcgtcg tccatcgagc
    11461 ccgcctctcc ccgggtggcg gtgacgatca cgacccggga gccgttgcgg acggcccgag
    11521 cggccaggcc ggccatcagg taggtctcgt catcgggatg cgcccagacc cccagcaccg
    11581 tcccgagctc gtccatcgct ccctccgctt cccgcgtgca ggccagggag catcctagcg
    11641 atgtcgtgga ggggccggac agcgaagaac ggcccgaagg ccgttcttcg gggtcccgcg
    11701 catcgggagg gatgagcgcg gggccggtca ggcccggggg tcgaccagcc gtttcccccg
    11761 gaagttgggg aaggtgatgg tggcgtgctg ccagcaggtt tccccggcgt tgtacatgga
    11821 cagccgcgtc ccgcagccgg gaacgcgaca gacgcgccct tcctcatccc acgtcttcga
    11881 tggcgccggc aggtgccccg gtcgcgttcc cttcatcatc ccgcggctcc tccttgcgta
    11941 cgtcgcatgg ccgggtggtt cgccgcccgc tattcgacgc gagccctcgg atggttcgag
    12001 ggggcgggag gatggtgcgc ggagctcggc ggacggcgac gcccagggcg accgggcctc
    12061 aggccggagc cggacctccg gggagctcga agagcacttc acggacccgg tccatgacgt
    12121 ggtccggaac ctcgccgcac ccgcacttgg ccctcaacag ctcccgcagg tgccgcgtga
    12181 actccgagtg gctcatacag gggctgcacc gcccgaggtg atcgctcacc tgcgagcgca
    12241 aggatggatc gagctcacca tcgaggtaga gctcgatctg cttgagcacg tcctggcagt
    12301 caatcgacga caagtcctcg ctcccgtacc gtctcccaca acgccttctc caaggccttt
    12361 cttccccggt gcaacctcga catgacggtg ccgatgggga tgtcgaggat ctccgcgatc
    12421 tccttgtagg agaacccctc gacgtcggcc agtagcaccg ccatgcggaa gccctcgggc
    12481 aacgcctcca gcgcacgctg cacgtcctcg tccggcaggg cgtccaggac ctcgctctcc
    12541 gccgaggact gggcccccga ctcccccagc ttgtcgtaca ggtaccaatc ctcgatgtcc
    12601 tcgtccgaca ccgtctgcgg ctgacgctgc cgcttccggt aggagttgat gaacgtgttc
    12661 gtgaggatgc ggtacatcca cgcccgcagg ttcgtcccct cctggaaccc gccgaatccc
    12721 cggtaggcac ggaggtagct ctcctgcacc aggtcctcgg cgtccgatgg gttgcgtgtc
    12781 agccgcagcg ccgcgccgta caggttcggc agcaagggga gcacgtcccg ctcgaaccgc
    12841 tcccgcatga cgggatcgtc gatcggggta ggacggctgc gcgccatggc agccagtatc
    12901 tcgaaatcct caggttcggc agggggaact cacagcgtgc acccggaaag cggactgtca
    12961 catgcctccg gggatgcgaa gatgggcgta tggcaggcgg cgacaacgac cgccggggcc
    13021 ggtggaccgg cttcgacttc ggtggtggct cccccgggga gccccgccgg ttccgcgcgt
    13081 ccgcgtggat ctggatcatc ggcttcctcc tgctcctgtt cctgatcaac tacctggccg
    13141 cgccgaggcc cacgaacatc gactactcgg tgttcctgca gaaggtccag caggggcaga
    13201 tcgtcggcac ggtccagatc tcacagacct ccgtgagcgg gacctaccag gaggggggga
    13261 agaacgtcga cttcaccgcg accctgccgc ccatcctgca gggcaccacc cagctcaccg
    13321 agacgctcca gcagaacgac atccggtaca cgggtgtgca gccgagcggt tggtccagcc
    13381 tcctcgtggg ctgggtggtc ccgctgctgc tgttcgcggc cctgtggatc ttcatcatca
    13441 gacgcatggg cggcgcccag acgtccgccg ccctgaacct ggggcggaac cgggtcaaga
    13501 tctacgaccg gaaggagatg aagaccacct tcgcggacgt cgccggcgtg gacgaggccg
    13561 tcgaggagct ccgcgaggtg gtggacttcc tcaagcaccc caagaagtac cagcgcctgg
    13621 gcgggcggat ccccaagggc gtcctcctgc tcggcccgcc gggctgcggc aagaccctgc
    13681 tggcccgggc cgtggccggc gaggccaacg tgccgttctt cttcatgtcg gggtccgagt
    13741 tcgtggagat gttcgtggga ctgggagccg cgcgggtccg cgagctcttc cagcaggcca
    13801 aggagaaggc gccggcgttg gtgttcatcg atgagctcga caccatcggc aaggggcgcg
    13861 ggggggcgct cggagccggc ttcggcgccc acgatgagcg cgagcagacc ctgaaccagc
    13921 tgctggtgga gatggacggg ttcgattcgt ccaagggcgt gatcatcatg gccgccacca
    13981 accgcccgga tgtcctggat ccggccctga cccgcccggg ccggttcgac cgtcaggtcg
    14041 tggtggatcg tccggacctg cggggccggg aggccatcct gcgggtccac gctcgcggcg
    14101 tggccatgga tccgggtgtg gacctgcggc tgctcgccgc ccggaccccc gggttcaccg
    14161 gcgcggacct ggccaacgtg gtgaatgagg ccgcgctgct cgccgctcgg gcggagaaga
    14221 acaccgtcac catgtccgag ctggaagagg ccatcgaccg ggtcacggcg ggcctggagc
    14281 gcaagagccg ggtgatgtcc gagcgtgagc gcgagatcgt cgccactcac gagatgggcc
    14341 acgcccttgt ggctctgaac atgcccacgg ccgacccggt gcaccgggtc tcgatcatcc
    14401 cccggggggc ggcggccctc ggcatgacga tgcagcgacc gctggaggat cgctacctgc
    14461 tcaccgagcc cgaactgaag gaccggctgg ccgtgctgct cgggggccgc acggccgagg
    14521 agctcggctt ccagcagatc tcaaccggcg cccagaacga cctccagcgg gccaccgaga
    14581 tctcccgggc catggtcacc gagtacggga tgagcgaccg ggtcgggccg ctgtccttcg
    14641 gcaacgacgg gttccgcagc ccggagggac gcatgctctt ccccggggcc gccccggagc
    14701 tgtcgcccga cctcgcggcc ctggtcgacg aggaggtcaa gcgcttggtg gacgaggccc
    14761 acaccagggc ccgcacggcg ctcgaggagc atcgcgacct gctggaccgc ctggccgacc
    14821 tgctcatggt caccgaggtg atcgaggggc cagacctgat ggcctacgtg gacgggtcca
    14881 ggcccatccc cacgctcgag gaagcgcggg aacggatcgc cgccggcaac ggggactcca
    14941 agacgaacgg gtcctcgggg aaggccaggc cggtctcgat gccaccgcct ccggtgcccg
    15001 tcgacccctc gtcctgatgg cccacgccgg agcggtccac cgcccgctcg ccccttagcc
    15061 gaaccaccga aatcgtcagg ggagcccgcg cagcagcttg gagaggcggc cggatggctc
    15121 gcccactggt tcctccaccg ggaccaggac gatggggcag gacaggtggc gggcgatccg
    15181 ctcctggggc aggagcgtca gccgcatcgc caggcggtcc ggggctcggg gcaggaccac
    15241 caggacgacc aggtccgcat ccacctcgcg ggcggtccgg atcaccgatc cggcgaaggt
    15301 ctccccgctc agcgaggccg agcggaccgg gatcccccgg tctcgggcca ggtcctccac
    15361 cgcccgcagc tcccccgtcc cctcctcgtc cggatgccga agcaccgtaa gggaggcccc
    15421 ggtccgggcg aaccagtcca ccagccaggc gggggccgtg gtccgtacct ccgaggcgag
    15481 caggatcgac gtgggcagtc gatcgtcgat cgggcccacg ccgtcctcgg gcacggttgg
    15541 catcgggggc tcggacctca ggccgcgggc cacgagcagg ggtccaacca gctcgaacag
    15601 caccacgctg cccagcacca ccgccgccac gtgcccgccg gtcccgggga ggctctccgc
    15661 cgccagaacc gccagcccca ccgccacgcc cgcctggggc atcaggtcca tccccagccg
    15721 cagcgagctc cggccgggga tgttccccgc gatccccacc agccagcccc ccgccacctt
    15781 gccggccgtg cgggccagca ggtaggccag ccccacgccc cccaacgcgg gaagcgcgct
    15841 gaagtggatg gatgcaccgg ccagcgcgaa gaacagcagg tagagcggct gttcgacctg
    15901 ccggaccgcc acgaacgttc gctgagcggc gtcagagggg cccagggagg ccgccaccgc
    15961 gcccatcacc agcgccgcga gcgggaggga gagccccagc gccgccgagg ccgaggccac
    16021 caccaggacg tgcacgagga tgaagagcag cagctctccc gagccctcga tcccggggcc
    16081 gaagcgtgca acgacccacc cgaagaacga tcccagcgcc accgtgccga gggccaccca
    16141 gccgaaccgg atccccgccg aggcgaggct ggtcccggga tggagcagct cctccacgat
    16201 gggtagggcg gccgcgaagg ccacggccgc cagggcgtcg cccaccgcgt ggaccccgat
    16261 cagcgcctcg ccgtacgggc tccgcccgcg ggtggatgcc acaacggagg ccaccgtcat
    16321 gggggccccg gcgcccgcca gcgcggccag gagcacggcg acgttgttcg tggctcccac
    16381 ccaccgggcc acagcgaaga ccagggccgc acagatcagg tactgcacga ggccggcgag
    16441 tcccacccac cgggcacggc ggagggcccg caccgacacc cgttccccca cggtgaacat
    16501 cagcactgcc agcgcgagct ggatcacacc ctcgagccgg cccaggtcct gcgcgtcaag
    16561 gaggcccagg acctccggtc ccatcagcgc cccggccccc aggaacacga tcagctcggg
    16621 gagggtccgg cgaagcagcc gaccggcgag gtacgccagc caggccagca ccgtgagcgc
    16681 gatcacgagg tcgctcatcg tggcatcgtt cccggaaccg gaccggaact caaccccccg
    16741 ccgggcggct ccccggtccc cgcaccaaca ggtcgaaccg gcctcggaag tggtccagga
    16801 agcggttgta gttcctcttg gacagcctgg cacgctcctc cggcggggtc gaggcccagg
    16861 cccgatgctc gtgcctgcgc accggcaccg ggaccacccg ggctcggagc cccgtatccc
    16921 tcacccggaa cgagtactcg atgtccgcgg tccggtacca gcggaaccgc tcgtcgaacc
    16981 ctcccacctc ggtgagcagt tcccggcgga aggccatcag gtagccctcg atcgcatcca
    17041 cctcgggccc ggacgacgcc tcgaagtccc gcaggtcgtc cgtgaccacg ccgaagggtc
    17101 cacacacccc cgtgtccgga tccacaagag cgttctctag cgggcccagg acgtccccct
    17161 cgggctccac cgagccgtcc atggccagga cgatccgccc cagtgaccgg cgcaggcccg
    17221 cgttgcgggc ggccgcccat cccgtgccct cctccaggct caccacctcc acgtccgcac
    17281 cgaacgtgga gggcgcggca cccgtcacgt cggcgaccac gtactggatc gttcgcggcc
    17341 cggcgaaccg ccggaacccc gcgagggcac gcctcacgtc ctcgggccac ccctgcaaga
    17401 cccagtgcac ggaggcgtcg gcgatggcgg gctcgtccag cagggagggg atcgccctcg
    17461 acgacgcccc ccgggcttgc tctcccccac cggccgcggg accgaggggc tcgaacgatg
    17521 gccccgcggg gccgtcgatc acccggtacc cgagctccga gatccgctcg cgcagggcat
    17581 cggcggggcc gaagtcccgc gcttgccgcc gctcctcccg ctccgccgcc agctcgcgga
    17641 cctcccgcgg gacctccgac tcaggcatcg cggtgcctcc ggaggaagtc caggacgacc
    17701 atgtccactc gctccggctg ctcccagtgg aagacgtgtc cggccccctt cagcagcacc
    17761 agctcggcgc cggcgatccg ggaggcgagc acctcggcgt tgcgggaatc gatcatccgg
    17821 tcgccggtcc catggatcac gagggtcgga gccgtgatcc tcggcagccg gtcgtaggtg
    17881 tcgaacccac ggatcgcctc acgctgccgg cgtgccgcgc cgggcacccc cgcgcgccgc
    17941 cggccgcgcc agcgctcacg gagctgctgc gggtgctcgg ccgcgaaccc ttccgagacc
    18001 agggccagtg aaggatcgcc cttcggggct cggtaggcgc tcgggatcac gtgatggccc
    18061 ccagcgtgtg tcgccccgag caccagcgcg cggacccgct cgggatgccc gagcgccagc
    18121 tcctgggcca ccatccctcc gagcgagatc ccgagcacgt gggctcgttc caccccgagc
    18181 tcgtccagca gggcgacggt gtcttcgatg aacgtcccca tctcgagagg gccggtgggg
    18241 ggatcggagc caccgttgcc acgcaggtcg taggcgacca cgaacagttc cgcggccagc
    18301 agcggcaggc tggaggccca gccggcgacg tcgccgccca gaccctcgag caggaccagc
    18361 ggcgaacggc gagggtcgcc gtggacctcg tagtagaggc cgcagccgtc gggacgctcg
    18421 agccgggcca cggagcgccc agcgctagac ctgctccttg gccaccgttc gcagacggcc
    18481 cgccaccgac tcgagggtct tgatggcgat gctggggtgc tcctggagca gcccgcggaa
    18541 cacccactcc gtgagtccgg ccagtcgcac gtccgtctcg gcggtgacgg tggccgagcg
    18601 gggaccgccg tcgaggagcg cgatctcgcc gaagaagtcg ccggccccca aggtcgccct
    18661 gcgcatgcca ccgatcgtca ccgagcagcg gccttccagg atcacgaaca gtcccaagcc
    18721 gcgctggccc tcgcgagcga tgatggtgcc ggagcggtgc gtgacctcct tggccgccct
    18781 ggccaccagc gccagctcct tctgggtgca gttggagaat atgggcacct gggcgatccg
    18841 ctcttccacg tccttcgcca cggcctcgac ctccctacgg cccacgcccg ggccgcctca
    18901 ccggcaggcg atcctatcgc gatcgccgtg gccggcgcga ggatcccggc cgctcactcg
    18961 tcggtgcccg agccctggcc gcggacggtc gcctccagcg tctcggacgc gatccggatg
    19021 tcggcgcggt ccaagcggtc gcggatccgc cgcaccatgc ggtccttcgt cgacctggcg
    19081 gtgcgaacgg ggaccacgaa gcgcgccgcc aactccatcc agttgtcggt ggctcgcgcg
    19141 aacacgcggg gctcgacctc ggcgcgaggg accgggtacc ggcgcaccat cgtggacatc
    19201 gcctcccgag cctccttgga ggcggagatc cgctctgcct cctcgcgcag gatgctctcg
    19261 gcgcgacgcc agtcggcgtc gtacgggatg gggaccacga gctcctccca gatgaagtcg
    19321 aagacggccg aatagttgta gacgggctcg gtgaaggtgg ccttgttgga gacggagacg
    19381 atccgaccgg tgtactgacg gccctgaacc caggaggcac cctcaactgg agaacccatc
    19441 tcgaggatct tcgtgcgcag gggcgtcacg tcgatcacgt cgcctcgcac cccgcccatc
    19501 tcgatccggt cgcccacggc gaagatccgc cccgacagga tgttgaacca gcccgccaca
    19561 gcgccgatca cctcctgcat cgcgaaggcg agaccggcgg ccaccacccc gaggaccagc
    19621 cccacccgtc cggcgaacgc gcgccagatc acgccgatgg ccaccagggc ggccacggcc
    19681 accacgtagt gggacaactt gcgcgcgtag tagcgggcgt acggatcatc gacccgcttg
    19741 cccagcagcc atcccgagaa ccatcccagg agcaacgccg ccgcgatggc gatcgccgtg
    19801 ctagccaggc gctcggggac cgtggcccct ccgacgatca gctcgacgac acggtccatc
    19861 ctgcccggag ggttcccgct gcccgcatcg gacaatccag tagggccggc gcggtccacc
    19921 cgggagggat gcgccggttg gcgcggcacg cctcccgggt agggagggtt catggagatc
    19981 taccaccacc cacccgcttg gctcgaggcg ctgtcctggg gctccctggc cgtggccttc
    20041 gccacggccg cggtcatcct ggccgatatc tacgggcggg gccaccggca gaagatgccg
    20101 atcatggagg ccgtctaccc catcagcgcc ctgtactggg ggccggtggc cctatggttc
    20161 tacctgcggt tcggacggag gaagtccaag aagatcatgg ccgagcgggc atcaagcccc
    20221 ggacccggcg ccatggcgat ggaggagcgg cccgagcagg gggagcgatc ctccgaggat
    20281 caggaggacc gggtccgctg gtggcaggtg gccaaggccg actcccactg cggggccggc
    20341 tgcacgctgg gcgacatcgg cgccgagtgg ctggtgttca cgctcggcct gctgatcgcc
    20401 gggaaggcgc tgtacgcgga cttcccgctc gacctggcct tcgcctggac cctgggggtg
    20461 gtgttccagt acttcacgat cgtcccgatg cgcgacctcg gacccctgag gggcatctgg
    20521 gccgccatcc gggccgacac gctctcgatc gtcgccttcc agatcgggct gttcgccggg
    20581 atgttcgtct atcaggagct gatcttcccc aaccccctgc ccaagacctc cgccacctac
    20641 tggatgttca tgcagctgtc gatgatcctg ggtttcttca ccgccctgcc cgcgaaccgc
    20701 tggctgctcc ggaagggctg gaaggagaag atgtaggggt ccggggtggt tgcggccgcc
    20761 gggccataaa cgccgccgcg accccggccg ccaccaggat cgagccggcc aggatcagga
    20821 cctggcccag accgttcgtg aaggcggtct gggccgcctg gatgaccttc tccacgagcg
    20881 cgccggtccc ggcgggagcg ccgggtgctc ccgaggccga ggtggggacg gcgccgtggg
    20941 tgatgccctg gatcacgatc tcccggaagc tcggcgggat gcccagttcc cgtaggcgct
    21001 gggccatatg ggaggtgatc gtggcgctca tgatcgcccc caggatggcc acaccgaaga
    21061 cgcctccgag ctcgcgactc gtgttcacgg tggatgcggc catgcccgag cgctgaggag
    21121 ggaccaccga caacaccgtg gcgttcatcg tgacaagggc catgccgaac ccgaacccga
    21181 ccaccgccag cgcccaggtc agcgagacca gggacacgct cggctgcagc agggcgttca
    21241 cgacgaacag cccgccgccg gccatgaggc atcccagcga catcggt
//
LOCUS       DASQQQ010000365        10546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_101270, whole genome shotgun sequence.
ACCESSION   DASQQQ010000365 DASQQQ010000000
VERSION     DASQQQ010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10546
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101270"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1145)
                     /locus_tag="VE646_13725"
     CDS             complement(3..1145)
                     /locus_tag="VE646_13725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD81083.1"
                     /translation="MLVNAGYLAVTTGESLLAPVFPIAARELGLDLREAGLAFALLAL
                     SIAIGNLVGGLLLWRRGPRPGALIGLGGATVGALLAASSGAWPVFLVAQVLLGLGSGV
                     FFAAGLSAAGALGGDRRGLAMGFFGVAFSGGLALAALLIAVGGSDHWRAAFAVSAAVS
                     AAALAGLTLARLPGRAARAQPVSWKGVHRELGIPLAVGGMAAASQYGTVSFLPSFAVQ
                     IWGLSPAAAAVLLAVARVGSIPGKVSAGHRADRRGGLATAGQIGVVLAALGIWWTLVP
                     WAPAALWAAVGFAAVVSALGPVANVLALDAFSDREILLGVFRSAQIGLGAVAGALLGA
                     FSPHVGLRPTLAVAAVAPLSLGLLARGARRRPVGGGTVDAGEGVDD"
     gene            1341..1808
                     /locus_tag="VE646_13730"
     CDS             1341..1808
                     /locus_tag="VE646_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81084.1"
                     /translation="MGKDIGDRLPHGVMTAFDGTSLERKIGPAYLLVSTDPDGRPRPC
                     MLSAGEVLATDDRHLRVGLWPGTHTSQNLARGASIVFCFVSPGTVLYIRGSVHPLPEA
                     PQAQLARFEIEVASVESDVHPGMPVTQTISFDVERNRSEVAANWRRQIEALRA"
     gene            complement(1830..2021)
                     /locus_tag="VE646_13735"
     CDS             complement(1830..2021)
                     /locus_tag="VE646_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81085.1"
                     /translation="MDRSVFVDETQTFEGQVAMKAFRASQAARWHRERELSREAGGRE
                     RRFRWLGPQQPARAARPAA"
     gene            complement(2148..2777)
                     /locus_tag="VE646_13740"
     CDS             complement(2148..2777)
                     /locus_tag="VE646_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011255032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HZD81086.1"
                     /translation="METEPRPPLIVAMTGATGTVYGIRLLEALRAAGRETHLVISEWA
                     ERTMVAETSWKPADVRSLADVVYEEDQLDAPPGSGTFRTSGMVVAPCSMKSLAAVANG
                     ISQNLIHRAAEVTLKEGRKLVLLVRESPLSVIHLENMLRVARTGGIIMPPVPAFYARP
                     TSIEEMVDHTVGRILDHFGIPHQLVRRWGEKRRTWTPRTLAGGPSSEDE"
     gene            complement(2821..3192)
                     /locus_tag="VE646_13745"
     CDS             complement(2821..3192)
                     /locus_tag="VE646_13745"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZD81087.1"
                     /translation="MQVMGLVWAGTATDRFDETVAFFRDVLGLSEFHGSEGLSVLRTP
                     GGEWVEVFGPGHEHFEEFDTGPVVEFLVDDLASARSELEGQGVEFLHENHTWGDYVWA
                     HFRGPDGKIYGITSGPYGSEA"
     gene            complement(3214..5634)
                     /locus_tag="VE646_13750"
     CDS             complement(3214..5634)
                     /locus_tag="VE646_13750"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZD81088.1"
                     /translation="MGAPAHPYARTSVETEPVVNPPRYVGQRLRRKEDERLITGRAQH
                     VGDMKVPGMLHVAVLRSPLPHAAITKIDASAAAAVDGVVGVFTWRDLEGAMGPFVEAA
                     RTEVSPVLMEKVGPTLKSCPMPVLAQGTVFWVGQPVAAVVATDRPVAEDALELIEVDY
                     DPLPVVADPQRALDPDAPVLHPELGDNVQTSFRVSAGDVAKAFAEAEHTLKARFVMGR
                     QVGNPMETRGILAVPDPGRDELTVWDANARPHLVRTFICSMLDMPQESVRVIGPDMGG
                     SFGTGMFPEDVLIPFLARWLQRPVRWIEDRREHLANTRHARDQIHDIEVAYRDDGTIV
                     GLRDRYLIDAGAYNPYAITVSYNSAAHFRGQFAIDHMELESLAVVTNKAPVTPVRGAG
                     RPEVVFVMDRVVDVIADRLGLDPIAVRRRNLIRLEQMPYPMGIPYRDGVDIVYDRADF
                     PGQLDQALELFGYETFRRRQEEGRARGRRLGVGVSSYVEGSGYGPHEGAMIRVDRRGR
                     VAVYTGAKPHGQGLETTLSQICADQLGVRPEDVTVRGGDTSMIAHGVGTFASRSAVTA
                     GAAVGVAAQRMREKILAVAGELLETDPRDLEIVDGRISPRGAAEPATNLAEVARAATP
                     GPRARLPEGHEPGLETTYYFVPPTVTFASGTHVVGVEVDEDTGFIKLLKYVTVDDCGQ
                     MLNPTVVEGQIMGGVAHGIGNAMLEEAVYDADGQLLTGTYMDYLMPTAADVPPIRVGH
                     QEFLSELNPFGIKGCGEGGAVSPPAAVANAVVDALRPLRIDVDRVPLHPERLFTLIEE
                     AKASGVQA"
     gene            complement(5636..6127)
                     /locus_tag="VE646_13755"
     CDS             complement(5636..6127)
                     /locus_tag="VE646_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZD81089.1"
                     /translation="MTDRRRITLTVNGRERTGEAEPRMTLADFLRDELNLTGTHLGCE
                     HGVCGACTILMDGHSVRSCLLFAVQAGGHELTTVEGLAADDGTLSPAQEAFSANHALQ
                     CGFCTPGFLIAVTEYLAEGAPEVDDEEALRARLSGNICRCTGYQNIVTAVRDAAGAGA
                     GGA"
     gene            complement(6124..7002)
                     /locus_tag="VE646_13760"
     CDS             complement(6124..7002)
                     /locus_tag="VE646_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HZD81090.1"
                     /translation="MKPAPFEYAAPQSLEEAVGLLQQHGDEAKVIAGGQSLVPLLAFR
                     LAAPSLLVDLNAIRELEYARLEGDTLVLGALARHRAVERMTELATRCPMLAEGVSVIG
                     HVAIRNRGTVGGSLAHADPAAEWPSLALALDAEIDVVGPGGARTVPASAFFESYFTTV
                     LTPEEIVREIRLHLPNGGRTGSAYLELARRHGDFALAGVGAMLTLDEGGLIADARVAL
                     IGVRDTAVRSAAAEGVLRGDAPSPELFAEAAEAVQDEIDPVSDVHGSSGYRRHLAEVL
                     VGRALRRAGERAEAGA"
     gene            complement(7078..8550)
                     /locus_tag="VE646_13765"
     CDS             complement(7078..8550)
                     /locus_tag="VE646_13765"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HZD81091.1"
                     /translation="MASLPPEQKAELGKDLQSFIRFLEKYYPNEILRVKQEIDPVFEA
                     TATLWRLENDRRYPLVIFANIRGAELPAVTNLHASFPRLAMAIGLPIDATPRDFILEY
                     MRREDNPIEPVTVDESQAPCKEVIYTGDEVDIWKMPMLKYHELDGGQMDPGFEEQRGR
                     YITLGYEIMKDPDTGIRNAGIYRLQAKSKSKFGIQISETAHGHYIMQKNLKHGRPTEM
                     AVAIGLHPAVELGFLSFTSIDVDEFAMAGAMLGEPLEMVKCETIDVEVPARAEIVLEC
                     EIHPTEREPEAPFGEYPGTYGPERNNPVVHVKAITMRKNAHYHSSFVGHWDNLLLSGV
                     TRNSMIYKACRLASGGVRAVYVPPSGRCRYICYVQMEKLIEGDPKNAAMAAFAADPFL
                     KYVVVVDEDVDITNDSQVWHAIAQRVRFHEDAFVVTHARGSPLDPASYDPAGGSHLVT
                     KVGIDATRKADFPLEITTPGVEDVDLDALFGDAWKQVRQD"
     gene            9118..9191
                     /locus_tag="VE646_13770"
     tRNA            9118..9191
                     /locus_tag="VE646_13770"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9152..9154,aa:Arg,seq:tct)
     gene            9341..9817
                     /locus_tag="VE646_13775"
     CDS             9341..9817
                     /locus_tag="VE646_13775"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZD81092.1"
                     /translation="MPTGTDATGTSATPAPSSRKPALTLLADVPLFSGLSRPQLERVA
                     DLAQEVGYGAGRMIVRAGTPGAAFYVIVSGRAKVVKGKIASARGEAELGPGDFFGELA
                     LLDGGPRSASVVAAAPLSTVRIERAPFRRMLREDPDIGLKLLEAMAVRMRGMVESL"
     gene            complement(9928..10416)
                     /gene="purE"
                     /locus_tag="VE646_13780"
     CDS             complement(9928..10416)
                     /gene="purE"
                     /locus_tag="VE646_13780"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HZD81093.1"
                     /translation="MEPVVGVVMGSSSDWETVKHATDTLDELGIAYEAKVLSAHRTPD
                     QLAQWAGSAEDRGLEVIVAAAGGAAALPGAVAAKTLLPVLGVPMESRLSGLDSILSMV
                     QMPGGIPVGTLAVGRAGAVNAALLAAAILARKHDEVRSALSAYRDRQTAKVLSTPDPS
                     KG"
BASE COUNT         1574 a         3889 c         3540 g         1543 t
ORIGIN      
        1 ggctagtcgt caaccccctc tccggcatcg acggttcccc ctcccaccgg acgtcgccgg
       61 gccccccgag ccagcaggcc gagcgacaga ggtgccacgg cggccacggc cagcgtcggg
      121 cggagcccga cgtgcgggga gaaggcaccc agcagtgcgc cggccaccgc ccccagaccg
      181 atctgtgccg agcggaacac gccgagcagg atctcccggt ccgagaaggc gtccagcgca
      241 aggacgttgg ccaccggccc gagggcgctg accaccgcgg cgaaccccac ggccgcccac
      301 agggccgcgg gggcccaggg gacgagggtc caccagatgc cgagcgcggc caggaccaca
      361 ccgatctgcc cggcggtggc caaccctccc cgccggtcgg cgcgatggcc ggccgagacc
      421 ttcccgggga tcgatcccac ccgagcgacg gccagcagca cagccgccgc cgccggcgaa
      481 agtccccaga tctggaccgc gaacgagggc aggaacgaca cggtgccgta ctgggaggcg
      541 gcggccatcc ccccgaccgc cagagggatg ccgagctcgc ggtgaacccc cttccacgag
      601 acgggctggg ctcgggccgc ccggccgggc aagcgagcga gggtcaggcc agccagagcc
      661 gcggccgaga ccgcggccga gaccgcgaag gcagccctcc agtggtccga tccgcccacc
      721 gcgatcagca gggcggccag cgccaggccc cccgagaacg caaccccgaa gaagcccatg
      781 gccaggccac gtcggtcgcc tcccagagcc cccgccgccg atagaccggc ggcgaagaag
      841 acgccggacc ccaggccgag cagcacctgc gcgacgagga acacgggcca tgcgccgctg
      901 gacgcagcga gcagcgctcc cacggtggct ccacccaggc cgatcagcgc ccccggccgc
      961 ggacctcgac gccagaggag caggccacca accaggttac cgatcgcgat ggacagcgcg
     1021 agtagcgcga aggccagccc ggcctcgcgt aggtccagcc ccagctcccg ggcggcgatg
     1081 gggaagacgg gggcgagcag cgactccccg gtcgtcaccg ccaggtaccc ggcgttgacc
     1141 agcatgaacg atgcccgatg gggtccaccc gggaccttgg cccatggccc gcgcgaggtc
     1201 ccccacccac gcgacgtccc tcgcccaccc gtcggatcca ccccgcgccc gtctccccgt
     1261 gccgaggaga tatcttatct tcgagatgat tcaccgagcg gtccgcaacc gccggagccc
     1321 cccgctgaga ggagcgagag atgggcaagg acatcggtga ccgcctgccc cacggcgtga
     1381 tgaccgcctt cgacgggacc tcgctggagc ggaagatcgg ccccgcctac ctgctggtga
     1441 gtaccgatcc cgatggacga ccgcgccctt gcatgctctc ggcgggtgag gtgctggcca
     1501 ccgacgaccg ccatctccgg gtgggactgt ggcccgggac ccacacgtcg cagaacctgg
     1561 ccaggggggc ttccatcgtc ttctgcttcg tctccccggg caccgtcctg tacatccggg
     1621 gctcggtcca tccgttgccg gaggcgccac aggcccagct cgcgcggttc gagatcgagg
     1681 tggcgtcggt cgagtccgac gtgcatccgg ggatgccggt cacccagacc atcagcttcg
     1741 acgtggagcg taaccggagc gaggtagcgg ccaactggcg acgacagatc gaggccctgc
     1801 gcgcatgacg cgccctatgg ggccgacgat caggcggccg gacgagcggc ccgtgccggc
     1861 tgctgcgggc ccagccaccg gaaccggcgc tcgcgccccc cggcctcgcg gctgagctcg
     1921 cgctcccggt gccaccgggc ggcctgcgat gcgcggaacg ccttcatcgc cacctgtccc
     1981 tcgaaggtct gcgtctcgtc gacgaagacc gaccgatcca tctcccgacc cttccctcga
     2041 gcacccccag catcgtggca cgaacggggg gagcctcggg gccgaccggc tcgctccctg
     2101 taggacgttc gtcgcgggtc ccttgggtgg tgccggcgtg accgccctca ctcgtcctcg
     2161 gaggatggac caccggccaa cgtgcggggg gtccacgtcc gtcgcttctc gccccaccgc
     2221 cggaccagct gatgagggat accgaagtga tcgaggatcc gaccgaccgt gtgatcgacc
     2281 atctcctcga tcgatgtcgg tcgagcgtag aaggccggga cgggaggcat gatgatcccg
     2341 ccggtgcgcg ccacgcgcag catgttctcc aggtggatca ccgatagggg cgactcgcgc
     2401 accagcagca ccagcttccg cccctccttg agggtcacct cggccgcccg gtggatgagg
     2461 ttctgggaga tgccgttggc gaccgccgcc agggacttca tgctgcaggg ggccaccacc
     2521 atacccgagg tgcggaacgt gccgctgccc ggcggggcat ccagctggtc ctcctcgtag
     2581 acgacatcgg ccaggctccg tacatcggcc ggcttccagg acgtctcggc caccatcgtc
     2641 cgctccgccc actcgctgat caccagatgc gtctcacgcc ccgcggcccg cagggcctcg
     2701 agcagtcgga tgccgtagac cgtccccgtc gctccggtca tggcgacgat cagcggcggc
     2761 cgtggttcgg tctccaccct catcctctcc cggaccgagc gacggcccgc ggggacgatc
     2821 ctacgcttcg gagccgtagg gtccgctggt gatcccgtag atcttcccgt cgggaccccg
     2881 gaaatgcgcc cacacgtagt ccccccaggt gtggttctcg tgcaggaact ccaccccctg
     2941 gccctccagc tcactgcgag ccgacgcgag gtcgtcgacc aggaactcca ccaccggacc
     3001 ggtgtcgaac tcctcgaagt gctcgtggcc cgggccgaag acctcgaccc actccccacc
     3061 cggggtcctc agcaccgaca ggccctcgga cccgtggaac tcggagagac ccaggacgtc
     3121 gcggaagaac gccaccgtct cgtcgaaccg atccgtggcc gtgcccgccc agaccagacc
     3181 catgacctgc atgtcgcccc ccttcgatgg gcatcaggcc tgtacgcccg acgccttggc
     3241 ctcctcgatc agggtgaaca gccgctccgg gtggagcggc acgcggtcga cgtcgatccg
     3301 caacgggcgg agggcgtcca cgaccgcgtt cgccacggcc gccgggggcg agaccgcccc
     3361 tccctcaccg caccccttga tgccgaacgg gttcagctcc gagaggaact cctggtggcc
     3421 cacccggatc ggtggcacgt cggccgccgt gggcatcagg tagtccatgt aggtgcccgt
     3481 cagcagctgg ccgtcggcgt cgtacacggc ctcttccagc atggcgttgc cgatcccgtg
     3541 cgccacgccc cccatgatct gcccctccac gaccgtcgga ttcagcatct gcccgcagtc
     3601 gtcaaccgtc acgtacttca agagcttgat gaatcccgtg tcctcatcca cctccacgcc
     3661 caccacatgg gtacccgagg cgaaggtcac cgtgggtgga acgaagtagt aggtggtctc
     3721 caggcccggc tcgtggccct cggggagcct cgcgcgcgga cccggggtgg cggcgcgggc
     3781 gacctcggcc aggttcgtgg ccggctccgc cgcgccgcgc ggcgagatcc tgccatccac
     3841 gatctccaga tcccggggat cggtctccag gagctccccg gcgaccgcca ggatcttctc
     3901 ccgcatccgc tgcgccgcaa cccccaccgc cgccccggcc gtcacggcgc tccggctggc
     3961 gaaggtcccc acgccgtgcg cgatcatgga cgtgtcgccg ccccgcacgg tcacgtcctc
     4021 gggccggacg ccgagctggt cggcgcagat ctgcgacagc gtggtctcca gcccttgccc
     4081 gtgcggcttg gcgcccgtgt agacggccac ccggccccga cggtcgacgc ggatcatcgc
     4141 gccctcgtgc ggcccgtacc ccgagccctc cacgtaggag gacacgccga cccccagtcg
     4201 ccgtcctcgg gcgcggccct cctcctgccg gcggcggaac gtctcgtagc cgaacagttc
     4261 cagggcctgg tcgagctggc ccgggaagtc ggcccggtcg tagacgatgt cgaccccgtc
     4321 ccggtacggg atccccatcg ggtagggcat ctgctcgagc cggatcaggt tccgtcggcg
     4381 gaccgcgatg ggatcgagcc ccagccggtc cgcgatcacg tcgaccacgc ggtccatcac
     4441 gaacaccact tcagggcgac ccgcgccccg caccggcgtg accggcgctt tgttcgtcac
     4501 caccgcaagg ctctcgagct ccatgtggtc gatcgcgaac tggccccgga agtgggccgc
     4561 cgagttgtag gacacggtga tcgcgtatgg gttgtaggca ccggcgtcga tcaggtaccg
     4621 gtcgcgcaag cccacgatgg tcccgtcgtc ccggtaggcg acctcgatgt cgtggatctg
     4681 gtcgcgggcg tgccgggtgt tggccaggtg ctcccgacgg tcctcgatcc accggaccgg
     4741 ccgctgcagc caccgtgcca ggaaggggat cagcacgtcc tcggggaaca tgccggtccc
     4801 gaagctcccc cccatgtcgg ggccgatcac gcggaccgac tcctgcggca tgtcgagcat
     4861 ggagcagatg aacgtccgca ccaggtgcgg ccgggcgttc gcgtcccaca cggtgagctc
     4921 gtcgcggccg gggtcgggca cggccaggat cccccgggtc tccatggggt tgcccacctg
     4981 gcgtcccatc acgaaccgcg ccttcagggt gtgctcggcc tcggcgaagg ccttcgcgac
     5041 gtcacccgcc gagacccgga agctggtctg gacgttgtcc cccagctcgg gatgcaggac
     5101 cggggcgtcc gggtccagcg cgcgctgggg gtcggccacc accgggagag ggtcgtagtc
     5161 gacctcgatc agctccaacg cgtcctccgc caccgggcga tcggtggcca ccacggccgc
     5221 gaccggctgc cccacccaga agacggtgcc ctgggcgagc acgggcatcg gacacgactt
     5281 cagggtcggc cccaccttct ccatcagcac cggagagacc tcggtgcgcg ccgcttccac
     5341 gaagggcccc atcgcgccct cgaggtccct ccacgtgaac acacccacca cgccgtcgac
     5401 cgccgcggcc gcggacgcat cgatcttcgt gatggcggcg tggggcaagg ggctccggag
     5461 cacggcgacg tggagcatgc caggcacctt catgtccccc acgtgctgcg cgcggccggt
     5521 gatgagccgc tcgtcctcct tgcgccgcag gcgctgaccc acgtacctcg gtggattgac
     5581 gaccggttcg gtctcgacgg aggtcctcgc gtaggggtgc gccggtgcgc ccacctcacg
     5641 cccctccggc cccggcacct gccgcgtcgc gcaccgcggt gacgatgttc tggtagccgg
     5701 tgcaccggca gatgttcccc gagagccgag cccggagcgc ttcctcgtcg tcgacctcgg
     5761 gcgcgccctc ggccaggtac tcggtgacgg ctatcaggaa cccgggggtg cagaagccgc
     5821 actgcagggc gtggttcgcg gagaatgcct cctgagccgg actgagcgtg ccgtcgtcgg
     5881 cggccaggcc ctcgaccgtg gtgagctcgt ggcccccggc ctggacggcg aacaacaggc
     5941 acgatcggac cgagtgcccg tccatcagga tcgtgcaggc accgcatacc ccgtgctcac
     6001 agcccagatg ggtcccggtg aggttcagct cgtcgcgcag gaagtccgcc agggtcatgc
     6061 ggggctcggc ctcgccggtc cgctcccgac cgttcacggt caacgtgatc cgccgccgat
     6121 ccgtcatgct cccgcctccg ctcgctcccc ggcccgccgc aaggctcgac ccacgaggac
     6181 ctccgccagg tggcggcggt acccggacga gccgtgcacg tcggacaccg gatcgatctc
     6241 gtcctggacg gcctctgccg cctcggcgaa cagctccggg ctcggcgcgt cccccctgag
     6301 caccccctcg gcggccgcgg agcgcaccgc cgtgtcccga acgccgatca gcgccacccg
     6361 agcatcggcg atgaggccac cctcgtccag ggtgagcatg gccccgaccc cggccagggc
     6421 gaagtccccg tgtcggcggg ccagctccag gtacgccgac cccgtccgcc cgccgttcgg
     6481 caggtgcaga cggatctccc gcacgatctc ctcgggggtc agcacggtgg tgaagtagct
     6541 ctcgaagaac gccgaggccg gaacggtccg ggcgccgccc ggtcccacca catcgatctc
     6601 ggcgtcgagg gcgagcgcga gcgagggcca ctcggccgcc gggtccgcgt gggccaggct
     6661 gccacccacc gtccctcggt tccggatcgc cacgtggccg atgacggaga ccccttcggc
     6721 cagcatgggg cacctcgtcg ccagctcggt catcctttcc acggcccggt gccgggccag
     6781 ggcaccgagc accagcgtgt caccctcgag cctggcgtat tcgagctccc ggatcgcgtt
     6841 caggtccacc agcagggacg gggccgcgag acggaacgcc agaaggggca ccaggctctg
     6901 cccgcctgcg atcaccttcg cctcgtcgcc gtgctgctgg agcagtccga cggcctcctc
     6961 caagctctgg ggcgccgcgt actcgaaggg tgcgggtttc accggcgtac cttcctcgaa
     7021 ccgtacgatg ttgggtggac gcggacgggg ctcgagctcc gcccgcgtcg cacgtcctca
     7081 gtcctggcgc acctgcttcc aggcatcacc gaacagagcg tccaggtcca cgtcctcgac
     7141 ccccggcgtg gtgatctcca gcgggaagtc ggccttgcgc gtggcgtcga tgccgacctt
     7201 ggtcaccaga tgggacccgc ccgccggatc gtaggacgcc gggtccaggg gcgacccacg
     7261 agcgtgggtc accacgaacg cgtcctcgtg gaaccggacc cgctgggcga tcgcgtgcca
     7321 gacctgcgag tcgttcgtga tgtcgacgtc ctcgtccacc acgaccacgt acttcaggaa
     7381 cgggtcggcc gcgaaggcgg ccatggccgc gttcttcggg tcgccctcga tcagcttctc
     7441 catctgcacg tagcagatgt accggcaacg ccccgagggc ggcacgtaga ccgcacggac
     7501 accgcctgag gccagacggc aggccttgta gatcatcgag ttccgggtca cgcccgagag
     7561 cagcaggttg tcccaatgcc ccacgaacga gctgtggtaa tgcgcgttct tgcgcatcgt
     7621 gatcgccttc acgtggacca ccgggttgtt gcgctcgggg ccgtacgtgc ccgggtactc
     7681 gccgaaggga gcctcgggct cgcgctcggt gggatggatc tcgcactcca gcacgatctc
     7741 cgcccgcgcc gggacctcga cgtcgatcgt ctcgcacttc accatctcga gcggctcgcc
     7801 cagcatggcc ccggccatgg cgaactcgtc cacgtcgatg ctggtgaacg agaggaaccc
     7861 gagctccacg gcggggtgca ggccgatggc cacggccatc tccgtgggac ggccgtgctt
     7921 caggttcttc tgcatgatgt agtgcccgtg cgccgtctcc gagatctgga tcccgaactt
     7981 gctcttgctc ttggcctgca gccggtagat cccggcgttg cggatgccgg tgtcgggatc
     8041 cttcatgatc tcgtagccga gcgtgatgta ccggccccgt tgctcctcga agcctggatc
     8101 catctggccg ccgtcgagct cgtggtactt gagcatcggc atcttccaga tgtcgacctc
     8161 gtcgccggtg tagatgacct ccttgcacgg ggcctggctc tcgtccaccg tcaccggctc
     8221 gatcgggttg tcctcgcgcc gcatgtattc gaggatgaag tcgcgcgggg tggcgtcgat
     8281 cggcaaaccg atggccatgg ccaggcgcgg gaagctggca tggaggttgg tgaccgcggg
     8341 cagctcggcc ccccggatgt tggcgaagat caccagcggg taacgccgat cgttctccag
     8401 gcgccacagc gtcgccgtgg cctcgaagac cggatcgatc tcttgcttca ctcggaggat
     8461 ctcgttcggg tagtacttct cgaggaagcg gatgaaggac tggaggtcct tgcccagctc
     8521 cgccttctgc tcaggtggca aactcgccat cgcacccccc actccgcggt gggaccgcct
     8581 cgggtcccgg cccggatatc ttaacgtcgt agtattctgc cgcactggag tcggccggcg
     8641 caagtcgccc gggaacgaac caccggttgc cgccggctcc gacgtcgccg tccggtagtc
     8701 gagaccggcc atcgggcagg aatccctacg ttcatgaaca ggttgatggg ctgcgggacg
     8761 ggacctggtg gtgtccgacc agggtcagga tcgcccgccg ccggttgctg aggaaggcct
     8821 cgcccacccg cgggaagagc ctgaggaggt gaacgcgggt gtgctgggcg ctcgcgtact
     8881 cggacggatc ggatgctcgc aaggcgaaga gatcgacgac ctgaccgccg tgcaggtcga
     8941 ccacccggaa ggatcggccc gggggcacct ggagcgccct cccctctcgg gccggaacga
     9001 ggatggactc caccgctcca gcatgaccgc tgaggcgttc ggtccccgca gtggaggctc
     9061 cccacttcct cggggatgga ctctgggccg agtggtgtcg ggtacgctgg acccgatgcg
     9121 ccggtagctc agttggacag agcggctgcc ttctaagcag cgggtcgcag gttcgagtcc
     9181 tgcccggcgc gcttcccgag gtgacccgac cggccttcgt cccctcggct cgtccccctc
     9241 cgccgcggaa ccgtccgttg atacgaggcg atgccgatcg agcgtggtct tcgcccaagg
     9301 tcgggttgtt gccgtggccc ccgtctgctc gaatgggtcc atgcccaccg gaacggacgc
     9361 gacggggacg agcgcgacac ccgcccccag ctcgaggaag cccgccctga cgctgctcgc
     9421 ggacgtcccg ctcttctccg gtctgtcccg gccccagctg gagcgcgtgg ccgacctggc
     9481 acaggaggtc ggctacgggg ccggccggat gatcgtgagg gcggggaccc cgggggccgc
     9541 gttctacgtg atcgtctccg gccgggccaa ggtggtgaag ggcaagatcg cctccgcacg
     9601 gggagaggcg gagctcggac ccggcgactt cttcggcgag ttggcgctcc tggacggtgg
     9661 gccgcgctcg gcgtcggtgg tggcggcagc cccgctgtcc accgtgagga tcgaacgagc
     9721 cccgttccgc cggatgctgc gcgaggatcc ggacatcggg ctcaagttgc tcgaggccat
     9781 ggccgtgcgg atgcggggca tggtggagtc gctgtagccg cggccgaacc gccggtccga
     9841 gcggcattgg tagcggtgga aggtcgaggc cgcctcatcg acctgcgggg gccctcacgc
     9901 accatgggcg ggtgaggtac gccatcgcta gcccttcgac gggtcggggg tggacaggac
     9961 cttcgccgtc tgccggtccc ggtaggcgga gagcgccgag cggacctcgt cgtgcttccg
    10021 ggccaggatc gcggcggcaa ggagggcggc gttcacggcg ccggccctgc ccaccgcgag
    10081 ggtacccacc gggatccccc ccggcatctg gaccatcgac aggatcgaat ccagccccga
    10141 cagcctggac tccatcggta cgccgagcac ggggagcagg gtcttcgccg cgaccgctcc
    10201 cggcagcgca gcggcaccac cggcggccgc cacgataacc tcgagccctc gatcctcggc
    10261 cgagccggcc cactgggcca gctgatccgg cgtccggtgc gccgacagca ccttggcctc
    10321 gtaggcaatg ccgagctcgt cgagcgtgtc cgtcgcgtgc ttcacggtct cccagtccga
    10381 gctcgaaccc atgaccaccc ccaccaccgg ctccacctgc atccctccgt tccgtcctca
    10441 tcggcgtccc gcgggggaag tgtagattct cgggcggacc cgtcctgcgc cagggtgcgg
    10501 gtcgtacggt ctccagcggt cgaccgggag gaggcatgtc cgatcg
//
LOCUS       DASQQQ010000366         8390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_273943, whole genome shotgun sequence.
ACCESSION   DASQQQ010000366 DASQQQ010000000
VERSION     DASQQQ010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8390
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273943"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VE646_13785"
     CDS             complement(<1..620)
                     /locus_tag="VE646_13785"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HZD81094.1"
                     /translation="MTSETFTFYVQPWYRKSPYYEATKRAGCKSWGLYNHMLLPTLYD
                     DPVTEYEALRSDVTVWDVAVERCVEISGPDAFELTNLVTCRDLTKVEVGRCRYVLLTA
                     ASGGIVNDPVLLRLAEDRFWLALADSDALLFVAGVAAGRGLDVEIHEADVAPLQVQGP
                     KSKDVVAALVGDGVRDLRYYRCAEAEVDGIPVVVGRTGWTGEVGYE"
     gene            complement(646..1188)
                     /locus_tag="VE646_13790"
     CDS             complement(646..1188)
                     /locus_tag="VE646_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018348103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HZD81095.1"
                     /translation="MQSLRGHLLIAGADLWDPNFRRTVVLVGHHDEEGAVGVVLNRST
                     EVAVDEAVPPLAELVAPGERLFVGGPVQPQAAVVVADFEQPERAEVLALGTIGFLPEE
                     AELEDVGGIRRARIFAGYAGWGPGQLEEELETDSWVVEPARPDDVFTPTPERLWGDVL
                     RRKGPAYDLLRLMPDDPTMN"
     gene            complement(1242..2390)
                     /locus_tag="VE646_13795"
     CDS             complement(1242..2390)
                     /locus_tag="VE646_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009604667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HZD81096.1"
                     /translation="MPRDPYSFRFYVASWYRKSPYWQRTVEAGCTSWDLYNHMLIPTL
                     YDDDEKEYWHLLEHVTLWDVAVERQVEITGPDAAAFTQLLTCRDLSRCEVLQCKYAPI
                     ISPDGGIVNDPILLRLEENHFWLSIADSDALLYAKGVQAFAGMDVAIREPDVSPLQVQ
                     GPKSKDVIRDLFGESVSSLRYYRCWRGELGDIPVVVSRTGWTGEVGYEIYLRDGSRGL
                     ELWDRVMAAGEPYAIRAIAPSDQRRLEAGIFNYGNDMGIEDTPFHVTGMERLVEFSAD
                     FIGKPALQRVAEEGVDRKLVGVEIGGEPFRLWLEDPWPVRHEDQVVGRLTSASHSFRL
                     GKNLGYAWVPIGLAEPGTELELVSPDGSVRAVTATLPFWDPNKEVPKS"
     gene            complement(2390..4000)
                     /locus_tag="VE646_13800"
     CDS             complement(2390..4000)
                     /locus_tag="VE646_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZD81097.1"
                     /translation="MENRYDAVVVGGGHNGLVAAAYLAMAGARVALCEARYKTGGAAA
                     TDRPWPDLPEFRVTTLSYVMSLMPDTIIRDLELQRHGYRVHPVGPYVVPFRDGRVMIE
                     YDDPKRNREEFARFSKVDADALERWDAWMGGLADVLGPLLMSTPPRVGSRKPSELWEQ
                     LRLAWRLRGLNVRTVGEVTRLMTMSVSDLLDRFFISDEVRAVMAINGLIGTWAGPHEP
                     GTGYVMAHHSIGDVGDGTLGSWGVPEGGMGAVAEALERSARALGAEIRTNARVERVLV
                     DGGRATGVVLEGGQELAAPIVVTACHPQITFLRQIDRAELPPEFVHDIEHWSSRSGVV
                     KINLAISKLPKLSGRPEWTDYSGGFEIAPSIEELETAFEEARHGRAATFPFSDGVIPT
                     TLDPSLAPPGAHVVSLFTQWVPHTWSQEPHREELEAYADRVVDAYEDVAPGFKGSILA
                     RQVIGPYDMEHEWGLIGGNIFHGELSADQLFHMRPAPGYADYRTPIAGLYQCSSATHA
                     GGGVCGIPAYNCVREIRKDRRRAKKGKR"
     gene            complement(4273..5493)
                     /locus_tag="VE646_13805"
     CDS             complement(4273..5493)
                     /locus_tag="VE646_13805"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD81098.1"
                     /translation="MARNPTVPRSAVRRLAVARLISITGGAAAYTALMFTVYERTHSA
                     AWLSATLFLTFGVSGFAAPVGGWIGDRFDRKRVMIASDLLGVACFGAMALAHDPAWLL
                     GLAFGSALVETPFWSASGAAIPNLVEPKELGWANGMVSLGKNVGIMVGPAAGGALLAV
                     IGPSWVFAANGLSFAVSALLVALVRGRFSVPQDQAPREHRGMRAGFVFIVRDRVLRTV
                     VLGWVALVGGMGVVMVADVPLVEHFHAGSLGYGLLITTWGAGSVAGSLAGRRLDEGKE
                     PRALILGMALIGASTGAIAVSPWFAPILVMGFVSGFGDAVAMVADQGIQQRRTPDAVR
                     SRVMAAAESISTISYAVCLAGAGAVLGAVGPQGAYAIGGITAMVGAGLVIPVLRSADT
                     ERSEPELEPAITSG"
     gene            complement(5725..7311)
                     /gene="serA"
                     /locus_tag="VE646_13810"
     CDS             complement(5725..7311)
                     /gene="serA"
                     /locus_tag="VE646_13810"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HZD81099.1"
                     /translation="MRVLVTERLSALGLDMLRRDFQVDVRPDLATGALVAQIPPYDAL
                     VVRSQTQVTAEVIEAGRNLKVVARAGIGLDNVDVEAATRRGVMVVNAPQSNIVSAAEH
                     TLALLLSQARNIPQANASLRAGRWERSRYQGVELQGKTIGVVGLGRVGALVAQRAAAF
                     GMRIIAFDPYVPKERAKEMGIELMPSLESLLVQADFITIHLPRTPDTEGLIGERELAL
                     VKEGARLVNTARGGIVDEAALAKAVEDGRVAGAALDVFASEPTTDSPLFCLDQVVVTP
                     HLGASTTEAQDKAGTSVAEMVSLALRGEFVPYAVNVSAGTEVAEVVRPFLPLAEKLGI
                     LLTGLARGAVHKVECQFLGRIAEADTRVITLSVLKGVLRGVVHEPVSYVNAPIIARER
                     GIAISEVRSAISTDYVNLVGLRAETDDGPVSVAGTLVGNRDAERVLQVLDYDIEMAPA
                     TYMLFFTYEDRPGIIGKVGTILGGRDINIATMDVGRPTRGGTALMGLTLDNPVPQDVI
                     DEIAQQVGAMAPPVFIVLPD"
     gene            complement(7322..>8390)
                     /locus_tag="VE646_13815"
     CDS             complement(7322..>8390)
                     /locus_tag="VE646_13815"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HZD81100.1"
                     /translation="YHRGPGFGRMLREVTESLQRLYRTRCDVVVMTSSGTGGLESAVA
                     NCCSPGDVVLVPLAGFFAERMAAIARAYGLEVRTIDYAWGQTVKGADISAALADAPAH
                     AVLLTHSETSTGVVHDVEAAARAAKDAGALVIVDAVSSLGAVPFDFDGWGIDVAVSGS
                     QKALSASPGLAFVAVSEAAWEAQSRAACPRFYFDWSLYKEYFARPDPESPWTPAISVL
                     QGLHAALRLYYQDGPEAAAARHELLSRAVKQGVRALGLDLFGEDPEQAWTVTAIRAPE
                     GIDANRICSQIRADFGIVLAPGQGPLKGKVFRIGHLGYYDRLDMLRGLAALEMTLGKL
                     GHPVKRGAAVAAAEDVFADGE"
BASE COUNT         1308 a         3156 c         2756 g         1170 t
ORIGIN      
        1 atctcgtagc cgacctcgcc cgtccatccg gtccgaccga ccacgaccgg gatcccgtcc
       61 acctcggcct cggcgcaccg gtagtagcgg aggtcgcgga caccgtcccc gaccagcgcc
      121 gcgaccacgt ccttgctctt cggcccctgg acctggagcg gagccacatc cgcttcgtgg
      181 atctccacgt cgagccctcg accggccgcc actccggcca caaacagcag cgcgtccgag
      241 tccgccagcg ccagccagaa ccggtcctcg gccagccgca gcaggacggg gtcgttcacg
      301 atgccgccgc tggccgcggt cagcaggacg taccggcatc gccccacctc caccttcgtg
      361 aggtcccggc acgtgaccag gttcgtcagc tcgaacgcgt ccggcccgga gatctccacg
      421 caccgctcga ccgcgacgtc ccagaccgtg acgtcggacc gcagggcctc gtactcggtg
      481 accggatcgt cgtacagggt gggtaggagc atgtggttgt acaggcccca gctcttgcag
      541 cccgcacgct tcgtcgcctc gtagtacggc gacttccggt accagggctg gacgtagaag
      601 gtgaaggtct cggacgtcat gcgggccctc ctcggctgtg ctcggctagt tcatcgtggg
      661 gtcatctggc atgaggcgca gcagatcgta cgccggtccc ttgcggcgca gcacgtcgcc
      721 ccacagccgt tcgggagtgg gggtgaacac gtcgtcgggc ctggcgggct cgaccaccca
      781 ggagtcggtc tccagctcct cctccagctg gcccggcccc caccccgcgt agccggcgaa
      841 gatccgggct cgacggatcc cccccacgtc ctccagctcc gcctcctccg gcaagaagcc
      901 gatggtgccg agggccagca cctcggcccg ctccggttgc tcgaagtccg ccaccaccac
      961 cgccgcctgg ggttggaccg gaccgcccac gaagagtcgc tcacccggtg ccacgagctc
     1021 ggccagcggc ggcaccgcct cgtccacggc gacctccgtg ctccggttca gcacgacccc
     1081 caccgccccc tcctcatcgt ggtggccaac cagcacgacc gtacgccgga agttcgggtc
     1141 ccacaggtcc gccccggcga tcaacaggtg cccgcggagg ctctgcaccc caccatcatc
     1201 gcatccggaa gggccggcct tcccaccggc ccccgcgagg gtcaggactt cgggacctcc
     1261 ttgttcggat cccagaacgg gagcgtcgcg gtcaccgccc gcaccgagcc atcgggcgag
     1321 accagctcga gctccgtccc cggctcggcc agcccgatgg ggacccaggc gtagccgagg
     1381 ttcttgccca gccggaagga atggctcgcg ctggtcagcc ggcccacgac ctggtcctcg
     1441 tgacgcaccg gccagggatc ctcgagccac agacggaacg gctcgccgcc gatctcgacg
     1501 cccaccagct tgcggtcgac gccctcctcc gccacgcgct gcagggccgg cttcccgatg
     1561 aagtccgccg agaactccac cagccgctcc atccccgtca cgtggaaggg cgtgtcctcg
     1621 atccccatgt cgttgccgta gttgaagatc ccggcctcca ggcgacgctg gtcgctgggg
     1681 gcgatggccc ggatcgcgta gggctccccc gcggccatga cccggtccca cagctcgagg
     1741 cctcgggagc cgtcccgcag gtagatctcg taccccacct cgccggtcca tccggtcctc
     1801 gacaccacca ccgggatgtc gccgagctcc ccccgccagc accggtagta gcggaggctc
     1861 gagaccgact cgccgaacag gtcccggatc acgtccttgc tcttcggtcc ctggacctgg
     1921 agcggagaga cgtcgggctc gcggatcgcg acgtccatgc ccgcgaacgc ctggaccccc
     1981 ttcgcgtaca ggagcgcgtc cgagtcggcg atcgagagcc agaagtggtt ctcctccagc
     2041 ctgagcagga tcggatcgtt cacgatgccc ccgtcggggg agatgatcgg cgcgtacttg
     2101 cactgcagga cctcgcagcg cgagagatcc cggcaggtga gcagctgcgt gaacgccgcc
     2161 gcatcgggcc cggtgatctc gacctggcgc tccacggcca cgtcccagag ggtcacgtgc
     2221 tccagcaggt gccagtactc cttctcgtcg tcgtcgtaga gggtggggat cagcatgtgg
     2281 ttgtacaggt cccagctggt gcagcccgcc tccaccgtcc gctgccaata cggcgacttg
     2341 cggtaccagg aggcgacgta gaacctgaac gagtaggggt ctctcggcat cagcgcttcc
     2401 ccttcttggc ccggcgacga tccttgcgga tctctcggac gcagttgtag gcggggatgc
     2461 cgcagacgcc cccgccggcg tgggtcgccg agctgcactg gtacaggccc gcgatgggcg
     2521 ttcggtagtc cgcgtatccg ggggccgggc gcatgtggaa gagctgatcc gcggatagct
     2581 ccccgtggaa gatgttgccc ccgatcagac cccactcgtg ctccatgtcg tagggaccga
     2641 tcacctggcg ggccaggatc gatcccttga accccggagc gacgtcctcg tacgcatcca
     2701 ccacccggtc cgcgtaggcc tcgagctcct cgcggtgggg ttcctgcgac caggtgtgcg
     2761 ggacccactg ggtgaacagg gagacgacgt gcgcgcccgg cggcgccagg gacgggtcca
     2821 gcgtggtggg gatcacgccg tcggagaacg ggaaggtggc cgcccggccg tggcgggcct
     2881 cctcgaaggc cgtctctagc tcctcgatcg acggcgcgat ctcgaagccg ccgctgtagt
     2941 cggtccactc gggccgtccc gacagcttcg gcagcttcga gatggccagg ttgatcttca
     3001 ccactccgga acggctggac cagtgctcga tgtcgtgcac gaactcgggg gggagctcgg
     3061 cacggtcgat ctgccgcagg aaggtgatct gcggatggca cgccgtgacc acgatgggcg
     3121 ccgccagctc ctgccctccc tcgagcacca ccccggtggc ccggccccca tccaccagga
     3181 cccgctccac gcgggcgttc gtccggatct cggctcccag ggcccgggcg ctccgctcca
     3241 acgcctcggc caccgcaccc atcccgccct cgggcacgcc ccacgagccg agggtgccgt
     3301 cgccgacgtc cccgatcgag tggtgggcca tcacgtagcc ggtacccggc tcgtgcggtc
     3361 cggcccaggt cccgatcagc ccgttgatcg ccatgaccgc ccggacctcg tcggagatga
     3421 agaagcggtc caggaggtcc gataccgaca tcgtcatgag ccgggtcacc tcgccgaccg
     3481 tgcggacgtt caggcctcgg agcctccagg ccagccgcag ctgctcccac agctccgacg
     3541 gcttgcgcga gccgacccgc ggcggggtgc tcatcagcag cggacccagc acatccgcca
     3601 gacctcccat ccaggcgtcc cagcgctcca gggcgtcggc gtccaccttc gagaaccgcg
     3661 cgaactcctc gcggttccgc ttggggtcgt cgtactcgat catcacccga ccgtcacgga
     3721 aggggaccac gtaggggccc accgggtgca cccggtagcc gtgccgctgt agctccagat
     3781 cacggatgat cgtgtcgggc atcaggctca tcacgtagga cagcgtggta acgcggaact
     3841 cgggcaggtc cggccaggga cggtccgtgg cggccgcgcc cccggtcttg taccgcgcct
     3901 cgcacagcgc gacgcgagca ccggccatgg ccaggtaggc cgcggcgacg agcccgttgt
     3961 ggccgcctcc caccaccacg gcgtcgtacc ggttctccat cagggatcct cctgaacgat
     4021 gattcagtca tcgaaccacg cgctgaaccg tggttcaact cgggtcccct ggggccggtg
     4081 cccttccggc agccgagggc acacgccgtc gatcgcgacc cgagcggacc gccgagacga
     4141 ccctggacgc gagtgtccca ccgatcgctt tgaagcgcgg gccggcacgt cggggcggac
     4201 ggacgcccgg cacgggggga cctctgccag gaaggcgggg catgacatct gcacgccctg
     4261 aacctcggcg gctcagccgg acgtgatcgc gggctccaac tccggctcgg accgttccgt
     4321 gtcggcggac cgcagcaccg ggatcacgag cccggccccc accatggccg tgatcccgcc
     4381 gatcgcgtag gcgccctgcg gcccgaccgc ccccagcacg gcgcccgccc cggccagaca
     4441 gaccgcgtac gagatggtgg agatggactc tgccgccgcc atcacccgac tgcgcacggc
     4501 atcgggcgtg cgccgctgct ggatcccctg gtcagccacc atcgccacgg cgtcaccgaa
     4561 cccggagacg aagcccatca ccaggatcgg cgcgaaccac ggcgagaccg cgatggcccc
     4621 ggtcgaggcg ccgatcagcg ccatgccgag gatcaacgcc cttggctcct tcccttcgtc
     4681 caggcgacgc ccggccaggg acccggccac cgagccggcg ccccaggtgg tgatcagcaa
     4741 cccgtagccc agggagccgg cgtggaagtg ctccacgaga ggcacgtcgg ctaccatgac
     4801 cacccccatg ccgcccacca gggcgaccca tcccagcacc accgtgcgca gcacccgatc
     4861 gcgcacgatg aagacgaacc ccgcccgcat cccacggtgc tcgcgcggcg cctgatcctg
     4921 gggcacggag aaccggcccc ggaccagggc cacgagcagt gcggagaccg cgaacgacag
     4981 gccgttcgcc gcgaacaccc acgagggccc gatcacggcc agcagggcgc cgccggccgc
     5041 cggccccacc atgatcccga cgttcttgcc cagcgagacc atgccgttgg cccagcccag
     5101 ctccttgggc tcgaccaggt tcgggatcgc ggcgcccgag gcggaccaga agggcgtctc
     5161 caccagagcg gagccgaagg ccaggcccag cagccaggcc gggtcatgcg cgagggccat
     5221 cgccccgaag caggccacgc cgagcaggtc cgaggcgatc atcacgcgct tgcggtcgaa
     5281 gcggtcgccg atccagccgc ccaccggcgc ggcgaagccg ctgaccccga aggtgaggaa
     5341 cagggtagcc gacagccacg cggccgagtg ggtccgctcg tacacggtga acatcaacgc
     5401 cgtgtaggcg gcggccccgc ccgtgatcga gatcagccgc gcgacggcca gccggcggac
     5461 ggcagacctg gggacggttg ggttccgtgc catgaagcgg ctccttcgtg gatcagtgga
     5521 tggacctgcg aggggtgagt cggatgcccg ccgggcggcg ggtcccggcg tcagagatcg
     5581 acgccgacgt tggttccgaa gatcaccgtg agtcgcttca tcgcccggcg agtgtagcag
     5641 gcgtgtccgt tgcgcagcag caccggtgcc cagccgagcg cgaccgctgg tcagccggca
     5701 acccgaccgc cggtcgaccg acgctcaatc ggggagcacg atgaagaccg gcggcgccat
     5761 ggcccccacc tgctgcgcga tctcgtcgat cacgtcttgg gggacggggt tgtcgagggt
     5821 gagccccatc agggcggtgc cgccccgcgt cggccgtccc acgtccatcg tggcgatgtt
     5881 gatgtcgcgc cccccgagga tggtccccac cttcccgatg atccccggtc ggtcctcgta
     5941 ggtgaagaac agcatgtatg tggcgggggc catctcgatg tcgtagtcga gcacctgcag
     6001 cacgcgctcg gcatcgcgat tgcccacgag cgtccccgcc accgagaccg gcccatcgtc
     6061 ggtctcggca cgcaggccga ccaggttcac gtagtccgtg gagatggccg agcgcacctc
     6121 cgagatcgcg atccctcgct cccgcgcgat gatcggcgcg ttcacgtagg agaccggctc
     6181 gtgcaccacg cctcggagca cccccttgag gacggacagg gtgatcacgc gggtgtcggc
     6241 ctcggcgatc cgccccagga actggcattc gaccttgtgc accgcgccac gcgcgaggcc
     6301 agtgagcagg atccccagct tctcggccag cggcaggaag ggcctgacca cctccgcaac
     6361 ctctgtaccg gccgaaacgt tcaccgcgta ggggacgaac tcaccgcgga gcgcgaggga
     6421 gaccatctcc gcgaccgagg tgccggcctt gtcctgggcc tcggtggtgg aggcccccag
     6481 gtggggcgtc acgaccacct ggtccaggca gaacagcggc gagtccgtgg tgggctcgga
     6541 ggcgaagacg tcgagcgcgg cgccggccac acgtccgtct tccacggcct tggccagcgc
     6601 cgcctcgtcc acgatgccgc cccgggccgt gttgacgagg cgggcgccct ccttcaccaa
     6661 cgccagctcc cgctcgccga tcagcccctc ggtgtccggg gtgcgcggga ggtggatcgt
     6721 gatgaagtcc gcctgtacca gcagggactc cagggagggc atgagctcga tccccatctc
     6781 cttggcccgc tccttcggca cgtacggatc gaaggcgatg atgcgcatcc cgaaggccgc
     6841 cgcccgctgc gccaccagcg cgcccacgcg acccaggccc accacaccga tcgtcttgcc
     6901 ctgcagctcg acgccctggt agcgggatcg ctcccaccgg ccggccctga gcgacgcgtt
     6961 ggcctgaggg atgttcctgg cctgggacag gagcagcgcc agggtgtgct cggcggcgga
     7021 gacgatgttg gactgcggag cgttcacgac catcacccca cgccgggtgg ccgcctccac
     7081 gtccacgttg tcaaggccga tcccggcccg ggccaccacc ttcaggttgc ggccggcctc
     7141 gatcacctcg gccgtcacct gggtctgtga ccggaccacc agggcgtcgt agggagggat
     7201 ctgggccacc agggcgccgg tggccaggtc gggtcgtacg tccacctgga agtcccggcg
     7261 cagcatgtcc agccccaacg ccgacagccg ctccgtgacc agcacccgca tgctcggacc
     7321 cctactcgcc gtccgcgaag acgtcttcgg ccgccgccac cgccgcccct cgcttgacgg
     7381 ggtgacccag cttgcccagg gtcatctcca gcgccgccag tccccggagc atgtccagac
     7441 ggtcgtagta gcccaggtgc ccgatcctga acaccttgcc cttgagcggt ccctgccccg
     7501 gcgccaggac gatgccgaag tcggcccgga tctgagagca gatccggttg gcgtcgatgc
     7561 cctcgggagc ccggatcgcc gttacggtcc acgcctgctc gggatcctct ccgaacagat
     7621 ccagccccag ggcccggaca ccctgcttca cggcccggga caacagctcg tgccgggcgg
     7681 ccgctgcctc gggcccgtcc tggtagtaca ggcgcaacgc cgcgtgcagg ccctgcagca
     7741 ccgagatggc cggcgtccac gggctctcgg gatcgggccg cgcgaagtac tccttgtaca
     7801 ggctccagtc gaaatagaag cgggggcagg cggcccggga ctgcgcctcc catgccgcct
     7861 ccgagacggc cacgaaggcc agccccggag aggcggacag ggccttctgc gacccgctca
     7921 ccgccacgtc gatcccccag ccgtcgaagt cgaacggcac cgcaccgagc gaggacaccg
     7981 cgtccacgat caccagggcc cccgcgtcct tggcggcgcg ggccgcggcc tcgacgtcgt
     8041 gcacaacgcc cgtggacgtc tcgctgtggg tgagcagcac ggcgtgcgcc ggcgcgtcgg
     8101 ccaacgccgc cgagatgtcg gcgcccttga cggtctgccc ccacgcgtag tcgatggtcc
     8161 ggacctcgag gccgtacgcc cgcgcgatcg cggccatgcg ctccgcgaag aacccggcca
     8221 acgggaccag gaccacgtcg cccggcgagc agcagttggc gacggccgac tccagccctc
     8281 ccgtgcccga cgaggtcatc acgaccacgt cgcaccgcgt ccggtacagg cgctggaggc
     8341 tctcggtgac ctcacgcagc atccgcccga agcccggacc tcggtggtag
//
LOCUS       DASQQQ010000367         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_26943, whole genome shotgun sequence.
ACCESSION   DASQQQ010000367 DASQQQ010000000
VERSION     DASQQQ010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26943"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VE646_13820"
     CDS             <1..309
                     /locus_tag="VE646_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010033513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HZD81101.1"
                     /translation="SPYMVFVHRVRPGWAERIAAVVHVDGTARIQTVDPADDPLVGGL
                     LQAFERKTGLPVLVNTSLNTAGRPIVDSPRDALECFGSAPIDALAIGPFIVRRTTLFR
                     "
     gene            306..1292
                     /locus_tag="VE646_13825"
     CDS             306..1292
                     /locus_tag="VE646_13825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZD81102.1"
                     /translation="MTATGVEIVIPTLGRPSLARLLQALGSGTRPDRIHLVDDRRKPD
                     RPLLPGPLPLIQPLLCLHRGGGHGPAAARNQGWRCARSRWVAFLDDDVIPQAGWMAAL
                     IDDLDSVGDDVAGTQGRLRVPAPRGRPATDWERNVSRLEEARWITADMAFRRAALESV
                     AGFDESFGRAYREDTDLALRLLERGWRLARGSREVTHPVRPASPWVSVRLQRGNADDV
                     ILTARHGRGWRQKVGEGPGRRRRHLLVVALGATALAVRRRPIAMLAATGWVAGTTSFA
                     LERIRPGPRDAGEVAKMVATSIVIPPAATFHWLRGLVTVLRHARLSVSAASG"
     gene            complement(1299..2150)
                     /locus_tag="VE646_13830"
     CDS             complement(1299..2150)
                     /locus_tag="VE646_13830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017607077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZD81103.1"
                     /translation="MPSGVSVVVATRDRRQPLLDTLGRLTSLPESPPVVVVDNASHDG
                     TVEAVRHRFPSVDVIALDRNLGAAARNVGVACAPTGLVAFSDDDSWWEPGALARSQEI
                     LERRPTVAGAAARILLPGSGREDPVCEAMRQTPLASDGLPGPRVLGFLACAAVVRREA
                     FMAVGGFWPPLRIGAEEKLLAIDLAMAGWPLVYREDLVAYHRPAGRDPWARRRQIARN
                     ELWISWIRRPVATAVGKTREMIGRGRSDAAVAAGVRRGLAGLPRVLGHRRVVAPALES
                     DLQLVGA"
BASE COUNT          320 a          850 c          739 g          312 t
ORIGIN      
        1 agcccgtaca tggtcttcgt ccaccgggtg agacccggtt gggccgagcg catcgccgcg
       61 gtggtgcacg tcgacggcac cgctcgcatc cagacggtgg atcccgccga cgacccgctg
      121 gtgggagggc tgctccaggc cttcgagcgc aagaccgggc tcccggtgct cgtcaacacc
      181 agcctcaata ccgccgggcg gccgatcgtc gacagccccc gcgacgccct ggagtgcttc
      241 ggatccgcac cgatcgacgc actggcgatc ggtccgttca tcgtccgtcg taccaccctc
      301 ttccgatgac cgcgaccggt gtggagatcg tcatcccgac gctgggccgg ccctccctgg
      361 cgcggctcct ccaggctctc gggtcgggca ctcgccccga tcgcatccac ctcgtcgacg
      421 accgccgcaa gcccgaccgg cctttgctcc ccggccctct ccctctcatc cagcctctcc
      481 tctgtcttca ccgcggaggc ggccacggac cggccgcggc ccgcaaccag ggttggcgct
      541 gtgcccgctc ccggtgggtg gcgttcctcg acgacgacgt catcccgcag gcgggctgga
      601 tggcggccct catcgacgac ctggactcgg tgggtgacga cgtcgccgga acccagggac
      661 gtctcagggt gccagctccc cgtggccgcc cggcaaccga ctgggaacgc aacgtctccc
      721 gtctcgagga ggcgcgctgg atcactgccg acatggcctt ccgcagggcg gctctggaat
      781 cggtggcggg attcgacgag agcttcggac gcgcctaccg ggaggacacc gatctcgccc
      841 tccggttgct ggagcgaggc tggcgcctcg cccggggaag ccgcgaggtc acccatcccg
      901 tgcgacctgc ctccccgtgg gtgagcgttc gcctgcaacg ggggaacgcc gacgacgtca
      961 tcctcaccgc tcgccatggt cgaggctggc gccagaaggt cggcgagggc cccggccggc
     1021 gacggcgtca cctgctcgtc gtggccctgg gcgcaaccgc cctggcggtc cggcgacggc
     1081 cgatcgcgat gctggcagcc accggctggg tcgcaggcac cacctcgttt gcgctcgagc
     1141 gcatcagacc gggaccccgc gatgccggcg aggtggcgaa gatggtggcc accagcatcg
     1201 tgatccctcc cgcagctacc ttccactggc tgcgggggct ggtgacggtt ctgcgccacg
     1261 cccgcctctc ggtgtcggca gcctccggct gacccggctc aggcaccgac cagctgcaga
     1321 tcgctctcca aggcgggagc gaccacacgg cggtgcccca gcacccgggg caggccggca
     1381 agcccccggc ggacccccgc tgccacggcc gcatcggacc tgccccgccc gatcatctct
     1441 cgggtctttc cgaccgccgt cgccaccggt cgacgaatcc aggagatcca caactcgttg
     1501 cgggcgatct gcctccgacg cgcccacggg tcgcgtcccg ccggtcggtg ataggcgacg
     1561 aggtcctccc ggtacaccag tggccagcct gccatcgcca gatcgatggc cagcagcttc
     1621 tcctccgctc cgatccggag aggaggccag aacccgccca ccgccatgaa tgcctcgcgc
     1681 cggacgaccg ccgcgcaggc aaggaagccg agaacccgcg gacccgggag gccatcggag
     1741 gccagcggtg tctgccgcat cgcctcgcag accggatcct ccctcccgct ccccggcagc
     1801 aggatccggg cggcggcgcc ggccaccgtc gggcggcgct ccaggatctc ctgtgaccgg
     1861 gccagcgcac ccggctccca ccaggagtcg tcatcgctga aggcgacgag tccggtgggg
     1921 gcgcaggcga ctcccacatt ccgcgccgcg gcgccgaggt tgcggtcgag agcgatcacg
     1981 tcgaccgacg ggaaacggtg cctcaccgcc tccaccgtcc cgtcgtgaga ggcgttgtcg
     2041 accaccacca ctggaggtga ctcggggaga gacgtcagcc ggccgagggt gtcgagcagc
     2101 ggctgccgcc ggtccctggt ggcgaccacg accgagacgc cggaaggcat ggaccccgct
     2161 gtacccggat cctgtcggtc cgacgccgac gttttgcccg atacgactcc gggaacaagc
     2221 a
//
LOCUS       DASQQQ010000368         9924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_410869, whole genome shotgun sequence.
ACCESSION   DASQQQ010000368 DASQQQ010000000
VERSION     DASQQQ010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9924
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410869"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VE646_13835"
     CDS             <1..288
                     /locus_tag="VE646_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81104.1"
                     /translation="SREQAERVQSCRELCLSYEEEGTTGELDGRLPAAEMAVVQARLE
                     QVASTLPVMPGEQDRSFTPPGWPMPWWRCARERVPGAPAPPSWSRGGLGLR"
     gene            266..505
                     /locus_tag="VE646_13840"
     CDS             266..505
                     /locus_tag="VE646_13840"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HZD81105.1"
                     /translation="MEALGSGERSAQVGTGGVIASPTLHRLLCKARLQLAVQDRTGTP
                     SYLSTPAREPTGAMLRLLRHRDPECRFPGCGPGPT"
     gene            536..697
                     /locus_tag="VE646_13845"
     CDS             536..697
                     /locus_tag="VE646_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81106.1"
                     /translation="MGPPPFPNLVLICTFHHRLVHEYGWGLVRGDDGELRWYRPDGVR
                     YRAGPALAA"
     gene            831..1388
                     /locus_tag="VE646_13850"
     CDS             831..1388
                     /locus_tag="VE646_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003988832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZD81107.1"
                     /translation="MHETRRMRHEGPHHHGGPPPWVRHGRGSWFGGSWGGPRRARRGD
                     VRTALLALLDERPMHGYDLIRELEERSGGAWRPSPGSVYPTLQMLEEEGLIAGEDQDG
                     KRVFSLTDAGRADLAERRERDDGSAPWEIGGFGEAVGQLRDAMFSLGAAAMQVARTGT
                     ESQRKKAAEVLAEARKKLYTMLAEG"
     gene            1740..2735
                     /locus_tag="VE646_13855"
     CDS             1740..2735
                     /locus_tag="VE646_13855"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HZD81108.1"
                     /translation="MPTGILERLADGPVLGDGGYLLELEKRGYVQAGPFTPEVVLDHP
                     EALEQLHREFLRAGVDVLQTMTFYASDDKLATVGLQGKVDEINRNAVRIAREVAAEGD
                     ALVAGNLSLTWAYDPADAATADHVRTLFDRQLQDQLDAGPPDFWIGETFGYLGEALLF
                     VERAKATGLPVMVTMSFEQPEPRAYEGDTPAQCAKRLAAAGADIVGVNCLNDPEQQLP
                     IAEAMRAAVSTHVASQPVAYRTTADKPDFTAWEAFPYALDPLQLSRQEMARFAGLARD
                     AGIDYIGSCCGSVQAHVRAMAKVLGKLPERERDWRSADGKAMSAYEYYAHTETEV"
     gene            2738..3910
                     /gene="solA"
                     /locus_tag="VE646_13860"
     CDS             2738..3910
                     /gene="solA"
                     /locus_tag="VE646_13860"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HZD81109.1"
                     /translation="MAERREFEIAVIGLGGIGIGALYWASRRVGGGALGLERFELGHD
                     RGASQDHSRIIRLSYHTPAYVRFARDAYAAWAEVEEESGQQLVIRTGGLDLYPESAHP
                     LMSDYTRSLDDVGVPYEWMDAGEAMRRWPQWRLGDDVQVMFQEASGIATAARANAAHR
                     RLAEARGAVMVEHARVTQIQEAGGLYELETEAGTYRAERLVIAADAWTNELLEQLWRP
                     IDLTVTQEQVHYYASTDLEAFAPERFPIWIWMGEPSYYGFPAYGEPGAKIGWDVGGRE
                     VTGDTRTFEPDPAYGAGLDAFMREHLPGGFGPYLAQKSCLYTMTPDRDFVLDLVPGHE
                     HVALGQGAAHAFKFASVFGRSLVELAFDAKTSSDVSAWSIRREILTSDRPLRDFLL"
     gene            3978..4514
                     /locus_tag="VE646_13865"
     CDS             3978..4514
                     /locus_tag="VE646_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZD81110.1"
                     /translation="MTTRPRDLSAGPDGLLPAERRVHQQLGHLPLDFVAMAAVSNLFR
                     ASVAVRRHMERKVLAEDRLSWTSFVVLWVLWVWGEMESRDLAAAVGISRPTSTGVVTT
                     LEKRGWARRRRNAEDGRMVRVSLTAMGERKIAALFPRFNEEERTVTARLSQEEQESLA
                     ALLRSLLRTVSPDGSEPG"
     gene            complement(4535..5113)
                     /locus_tag="VE646_13870"
     CDS             complement(4535..5113)
                     /locus_tag="VE646_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018832749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZD81111.1"
                     /translation="MGESGTRAAILEAARRRFAEHGYQGATIRSIAAEAGVDPALVHH
                     FFQSKERLFVIAMRFPIVPSELLSGLSDVDRDQLGPALVRAVLEVWERPETRSQSLAL
                     IRSALTNERAATMLRGFVTGAILDAVAGFTAAGDAEYRASLVGSQIVGLAVARYMVGL
                     EAIVDAARDDLVAAVGATVQRYLTGPVRAPAS"
     gene            complement(5185..6048)
                     /locus_tag="VE646_13875"
     CDS             complement(5185..6048)
                     /locus_tag="VE646_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD81112.1"
                     /translation="MSTLARTLDVTRRFGSLVAVDRVDLSVGPGEVVGLLGANGAGKT
                     TLIRLLLGLLPPTEGSVGLFGSPPSRGTRRRIGYVPQGLGLYDDLTPQENLAFSQAAF
                     GAAPATLPPALEPLSRVPVGGLPLGVQRQVAFLQALAHGPDLLVLDEPTSGVGPLARA
                     RLWETVRGAAEGGAGVLVTTHHMDEAEECDRLVVMAAGRVVAEGTMSHIVGDARVSVV
                     ETERWAEAFEALEAAGLPAALVGRTLRVPGAPPDRVAGALGHVRATVHDAAATLEERF
                     LELTLAGQGTG"
     gene            complement(6045..6896)
                     /locus_tag="VE646_13880"
     CDS             complement(6045..6896)
                     /locus_tag="VE646_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD81113.1"
                     /translation="MSWGVENLTVRSGERVALRDVTLEAANGGVTAVVGGDGAGKTTL
                     LRCLAGALRPDSGTVRRPEVRRIGYVSSTSGTYPDLSVEENLDFAARAYGVPMAAARQ
                     RVTELLERTDLAEARDRLAGHLSGGMRQKLGVIRAMIHRPDLLVLDEPTTGVDPVSRA
                     DLWWLIARGAAEGAAVVLSSAYLDEAERASWVLALDAGAVLAEGTPDRIAEALPGVIR
                     TAATRPDGEAGIRAWRRGASWRVWSPGEDAAPSGAQVARPDLQDAVTVAALVRELASA
                     HPGGGIP"
     gene            complement(6893..7969)
                     /locus_tag="VE646_13885"
     CDS             complement(6893..7969)
                     /locus_tag="VE646_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZD81114.1"
                     /translation="MWAMIVKEFRQLRRDRRTLAMMIFLPILMLVVFGYAARFDVQRI
                     SVDVVGPQAARVADALPAPFEVRTTDPAAGRAEAVAALRDGDVPVAIVTGSSGVTVLL
                     DGSELFIARAARASLSTLAERTASGVSTSVQVLFNPRLETAAIMVPGLAAMILLFVGT
                     VITSLGVVRERQAGTLEQLAVMPFRPSDVFLGKIAPYFAVAAVDMIVIVVAGIWLFDV
                     PFRGSVLVLTLGALLFLFVTLGIGVLISSVSENQGQAIQLALMTLLPQVMLSGLIFPL
                     SSMATGVRWIGYVLPLTYFIQIARGVMLRGASITALWAPFLYLAIIGVVVFGLATLRF
                     RRSLAPAQSRRHRGAEPALEGVST"
     gene            complement(8054..8545)
                     /locus_tag="VE646_13890"
     CDS             complement(8054..8545)
                     /locus_tag="VE646_13890"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZD81115.1"
                     /translation="MSPRAAWRLEALGFSEVYDYWPGKQDWFAAGLPSEGEVTSLPRI
                     GGIARRDVPTCLPHETVAAARSRVEGTIFDRCLVVTERRIVQGQLRVGKELEADPERA
                     AGEVMLPGPSTFRPNVSLREMAHFMDEHDFPRGAVTTSSGELIGEIRRDDVEGALAAA
                     PDP"
     gene            complement(8549..8752)
                     /locus_tag="VE646_13895"
     CDS             complement(8549..8752)
                     /locus_tag="VE646_13895"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZD81116.1"
                     /translation="MPTFIQRDRVRELIDRGAQLVDVLGREEFEESHLPGAINIPLRR
                     IDREARERLRTDAPVIVYCDDYS"
     gene            8900..9112
                     /locus_tag="VE646_13900"
     CDS             8900..9112
                     /locus_tag="VE646_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81117.1"
                     /translation="MTHLFDLADRFYRGGMERALFLRAERGPDGRGTFCLVRGEPLPT
                     AFGQDAYRRVFGSKVGTPSPSYSAER"
     gene            9109..>9924
                     /locus_tag="VE646_13905"
     CDS             9109..>9924
                     /locus_tag="VE646_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD81118.1"
                     /translation="MTEPAVGTSSTRASTRTRAVRFVLLVGVMSLFADFTYEGSRSIV
                     GQYLGTLGAGALAVAVVSGFGELLGYGLRLVSGRAADRTGRYWPITIGGYVLQMSVVP
                     LLALAGSWPLAAALVILERVGKATRNPPRDAMLSHAAGEMGYGWGFGVHEALDQLGAV
                     FGPLLVALILAVGHHDYRLAFASLAIPALIMLSLLGVARMLYPRPQDLEAGPGRVSGE
                     GLPRVFWVYLAGAALVGAGFADFPLIAFHFQSARTVSTTEIPLLYAAAMAVDGV"
BASE COUNT         1446 a         3538 c         3570 g         1370 t
ORIGIN      
        1 agccgggaac aggccgagcg ggtccagtcc tgccgggagc tctgcctcag ctacgaggag
       61 gagggcacca ccggggagct ggacgggcgc cttccggccg cggagatggc ggtggtgcag
      121 gcccgcctgg agcaggtggc atccaccctg ccggtgatgc cgggggagca ggaccgctcc
      181 ttcacaccgc ccggttggcc gatgccctgg tggcggtgtg ccagggagag ggtgccggga
      241 gcaccggcac caccatcatg gtcccgtgga ggccttgggc tccggtgagc gctcggccca
      301 ggtgggcacc ggcggcgtga tcgcctcccc caccctgcac cggctcctct gcaaggccag
      361 gctgcagctg gcggtgcagg accgtacggg caccccgagc tacctgtcca ccccggcccg
      421 ggagcccacc ggggcgatgc tgcggctgct gcggcaccgg gaccctgagt gccgcttccc
      481 cggctgcggg cccgggccta cctgaaggcc caccacatgg cctgggtgag ccggggtggg
      541 cccaccaccc tttcccaacc tggtgctgat ctgcaccttc catcatcgcc tggtgcacga
      601 gtacgggtgg ggcctggtcc ggggggacga cggtgagctc cgctggtacc gacccgacgg
      661 ggtccggtac cgagccgggc cagctctcgc agcctgagcg agcccatatc ttgccttcct
      721 tccgagcacc tatcggggag ccgcatatct cacacggccg atgtgttacg atatatcacg
      781 acaagtcgac gagggcatcg gcccccggcg acgggacgag gaggttgcag atgcacgaga
      841 cgcgacggat gcgtcacgaa ggcccacacc atcatggcgg gccaccgccc tgggtgcggc
      901 acggccgggg atcgtggttc ggggggagct ggggtggacc tcgaagggcc cgccggggcg
      961 acgtgcggac ggcgctgttg gccctgctgg acgagcgacc gatgcacggc tacgacctga
     1021 tccgcgagct cgaggagcgc tcgggtgggg cctggcggcc gagccccggc tcggtctacc
     1081 cgaccctcca gatgctcgag gaggaggggc tgatcgcggg cgaggaccag gacggcaagc
     1141 gggtgttctc cctcaccgac gccggccgag ccgacctggc cgagcgccgc gagcgcgacg
     1201 acggctcggc gccctgggag atcggagggt tcggagaggc cgtggggcag ctacgagacg
     1261 ccatgttctc cctgggggcg gcggccatgc aggtggcccg caccggcacg gaatcccagc
     1321 gcaagaaggc ggccgaggtc ctggccgagg cccgcaagaa gctctacacg atgctggccg
     1381 agggctagga gccgaagacc agggccgagg accagggccg aaggccaggt ggccgaaggc
     1441 cagggcctca agaggggggt ggcggccgcg tccgtcgcgg cagctgcggc cgccacccag
     1501 agggctctga ccctcagcgc acacccggag gggaccggag cgtgccttcc ctccaatcgg
     1561 tgtccccacg cctccggttg atgaggtcgc tcgaagcatc gacggaggtc ggggcgggtc
     1621 tcgaccttcg gcggaggcga tcgggggcga cccgtggtca ccttccgatg cggcgatggc
     1681 ccgctctgac cacctagaat cgccgttcca cgcacgcacg gggaaggggg acagggtcca
     1741 tgcccacggg catcctcgag cgcctggccg acggcccggt cttaggcgac ggcggctacc
     1801 tgctcgagct cgagaagcgc gggtacgtcc aagccggacc cttcacgcct gaggtggtgc
     1861 tcgaccaccc cgaggcgttg gagcagcttc accgggagtt cctccgggcc ggcgtcgacg
     1921 tcttgcagac catgaccttc tacgccagcg acgacaagct tgcgacggtc ggtctgcagg
     1981 gcaaggtcga cgagatcaat cggaacgccg tccggatcgc ccgagaggtg gcggccgagg
     2041 gtgacgccct cgtggcgggg aacctctcgc tcacctgggc ctacgatccg gccgacgccg
     2101 ccaccgccga tcacgtgcgc accctgttcg accgtcagct gcaggaccag ctcgacgccg
     2161 gtccgcccga cttctggatc ggcgagacct tcggctacct gggcgaggcg ctcctgtttg
     2221 tggaacgggc gaaggcgacc ggcctgccgg tgatggtgac gatgtccttc gagcagccag
     2281 agccgcgggc ctacgagggg gatacgcccg cccagtgcgc taagcggctc gctgccgcgg
     2341 gcgccgacat cgtgggcgtc aactgcctga acgatcccga gcagcagctg cccatcgccg
     2401 aagcgatgcg ggccgcggtc tccacccacg tggcgtcgca gcccgtcgcc taccggacca
     2461 cggccgacaa gcccgacttc accgcctggg aggcgttccc ctacgcgctg gacccgctgc
     2521 agctctcgcg gcaggagatg gcccggttcg ccggactggc gcgggatgcg gggatcgact
     2581 acatcggctc gtgctgcggc tcggttcagg cgcacgtccg agccatggcc aaggtcctcg
     2641 gcaagctccc cgagcgggag cgcgattggc gcagcgccga cggcaaggcc atgtcggcct
     2701 acgagtacta cgcgcacacc gagaccgagg tctgagcgtg gccgagcggc gcgagttcga
     2761 gatcgcggtg atcggcctgg gcgggatcgg catcggcgct ctctactggg cctctcgacg
     2821 ggtgggcggg ggagcactgg ggctggagcg tttcgagctc ggccacgacc gcggggccag
     2881 ccaggaccac tctcggatca tccggttgag ctaccacacg cccgcgtacg tccggttcgc
     2941 gcgggatgcc tacgccgcgt gggcggaggt ggaagaggag tccggtcagc agctggtcat
     3001 ccggaccggc gggctcgacc tgtacccgga gagcgcgcac ccgctcatga gcgactacac
     3061 ccggtcgctg gacgatgtgg gcgtgccgta cgagtggatg gacgcggggg aggccatgcg
     3121 gcgctggccg cagtggcggc tcggcgatga tgtgcaggtg atgttccagg aagcctcggg
     3181 catcgctacc gccgcccgcg cgaacgccgc tcatcggcgg cttgccgagg cccggggcgc
     3241 ggtgatggtc gagcacgccc gggtcaccca gatccaagag gccggcggcc tgtacgagct
     3301 cgagaccgag gccggaacgt accgggccga gcgcctggtg atcgccgccg acgcctggac
     3361 caacgagctc ctcgagcagc tctggcgtcc gatcgacctg acggtcaccc aggagcaggt
     3421 ccactactac gcctcgaccg acctcgaggc gttcgcgccg gagcgcttcc cgatctggat
     3481 ctggatgggc gagccttcct actacgggtt cccggcctac ggggaacccg gcgcgaagat
     3541 cggttgggac gtgggcggtc gcgaggtcac cggcgacacc cggacgttcg agccggaccc
     3601 tgcgtacggg gccgggctcg acgccttcat gcgcgagcac ctgcccggcg ggttcgggcc
     3661 ctacctcgcg cagaagagct gcctgtacac gatgacgccg gaccgcgact tcgtcctgga
     3721 cctcgtgccg gggcacgagc atgtcgcgct gggccagggg gccgctcacg ccttcaagtt
     3781 cgcgagcgtc ttcggccgct cgctcgtgga gctcgccttc gacgccaaga cctcgtcgga
     3841 cgtctccgcg tggagcatcc ggcgggagat cctgacctcg gatcgccccc ttcgggactt
     3901 cctgttgtag ccggcggtct ctcgccgtgc gctcggcgct cgccttcggg atggcgtccg
     3961 gcgataggat gcgcgacatg acaacccgcc ccagggacct ctcggcgggt ccggacggac
     4021 tcctgcctgc cgagcgcagg gtccaccagc agctcgggca cctgcccctg gacttcgtgg
     4081 ccatggcggc cgtctcgaac ctcttccggg cctcggtcgc ggtgcgccga cacatggagc
     4141 gcaaggtcct ggccgaggat cgactgtcat ggacctcctt cgtggtgttg tgggtgctgt
     4201 gggtgtgggg cgagatggag tcccgcgacc tggcagccgc cgtcggcatc agccggccca
     4261 cctccacggg cgtggtgacc acgctggaga agcggggctg ggcccgccga cggcggaacg
     4321 ccgaggacgg ccgcatggtg cgggtcagcc tcacggcgat gggggagcgg aagatcgccg
     4381 ccctcttccc ccgcttcaac gaggaggaac ggaccgtgac cgcccgcctg tcccaggagg
     4441 agcaggagtc ccttgctgcc ctcctccgtt cgctgctccg cacggtgtca ccggacggca
     4501 gcgaacccgg atagcccctg tagtctgggt cagactagct ggcgggcgcc cggaccggcc
     4561 cggtgaggta ccgctgcacg gtcgctccga ccgcggccac caggtcgtcg cgagccgcgt
     4621 ccacgatcgc ctccaggccc accatgtagc gggcgacggc cagccccacg atctggctgc
     4681 ccaccagcga ggcgcggtac tcggcatcgc cggcggccgt gaacccggcc accgcatcga
     4741 ggatggcccc ggtcacgaac ccccgcagca tcgtggccgc ccgctcgttg gtcagggccg
     4801 agcggatcaa cgccagcgac tgcgacctcg tctcgggacg ctcccacacc tcgaggacgg
     4861 cgcgaacgag cgccggcccg agctgatcgc ggtcgacatc gctcaggccc gagagcagtt
     4921 cgctgggcac gatcgggaac cgcatcgcga tcacgaacag ccgctccttg ctctggaaga
     4981 agtggtggac gagcgcaggg tccacaccgg cctcggccgc gatggagcgg atcgtggccc
     5041 cctggtaccc atgctcggcg aaccgtcggc gagccgcctc gaggatcgcc gcgcgggtcc
     5101 cgctctcgcc cacccgacga ccggtgcgac cggtgcgacc ggcgcgacca gtgccaccgg
     5161 tgcgagcggg gccaccggtc cggctcatcc cgtcccctgg ccggcgagcg tgagctccag
     5221 gaagcgctcc tcgagcgtgg ccgccgcatc gtgcaccgtg gcccgcacgt gaccgagggc
     5281 tcctgccacc cgatcgggcg gagcgccggg gacccggagc gtgcggccca cgagagcggc
     5341 cggcagtccg gcggcttcga gcgcctcgaa cgcctcggcc cagcgttccg tctccaccac
     5401 cgagacccgt gcgtcgccga cgatgtgcga catggtcccc tcggccacca cccgacccgc
     5461 cgccatgacc accaggcggt cgcactcctc ggcctcgtcc atgtgatgcg tcgtcacgag
     5521 gaccccggcg ccgccctcgg ccgccccgcg gaccgtctcc cacagccggg cccgggccag
     5581 cggacctacc cccgaggtgg gctcgtcgag caccagcaga tcggggccgt gcgccaacgc
     5641 ttgcaggaac gccacctggc gttggacccc gaggggcagg ccccccaccg ggactcgcga
     5701 caggggctcg agcgcggggg ggagcgtggc cggtgccgcc ccgaacgccg cctgggagaa
     5761 ggccaggttc tcctgtggcg tgaggtcgtc gtacagcccg agcccctgag ggacgtagcc
     5821 gatgcggcgt cgggtgcccc gcgagggcgg cgacccgaac agcccgaccg atccttccgt
     5881 gggcggcagc agccccaaca ggagccggat caaggtggtc ttgccggcac cgttcgcgcc
     5941 gagcagcccg accacctccc ccggcccgac cgacagatcc acccgatcca cggcgaccaa
     6001 tgagccgaac cgtcgagtga cgtccagggt ccgggcgagc gtgctcatgg gatcccccct
     6061 ccgggatggg ccgacgcgag ctcgcggacc agcgcggcga ccgtgacggc gtcctgcagg
     6121 tcggggcggg cgacctgggc tccgctcggc gccgcgtcct cccccggcga ccacacccgc
     6181 caggacgccc cgcggcgcca ggcgcggatc ccggcctcac cgtccgggcg ggtggccgcg
     6241 gtccggatca cgccgggcag ggcctcggcg atgcggtcgg gggtcccctc ggccaggacc
     6301 gcaccggcgt ccagggccag cacccacgag gcccgctccg cttcgtccag gtacgcgctg
     6361 gacaggacca ccgcggcgcc ctcggcggcc cccctggcga tgagccacca caggtcggcc
     6421 cggctgacgg ggtccacccc cgtggtcggc tcgtccagca ccagcaggtc cggtcggtgg
     6481 atcatggcgc ggatcacgcc gagcttctgc cgcatcccgc ccgacagatg gccggccagc
     6541 cgatcccggg cctcggccag gtcggtgcgc tccaggagct cggtcacccg ttggcgtgcc
     6601 gcggccatcg gcacgccgta cgcgcgagcc gcgaagtcca ggttctcctc cacgctgaga
     6661 tccgggtagg tgccggaggt cgaggacacg tacccgatcc ggcgcacctc gggccggcgg
     6721 accgtgcccg agtccgggcg cagcgcgccg gccaggcagc gcaggagcgt ggtcttgccg
     6781 gctccgtccc cgcccaccac ggcggtcacg ccgccgttcg ctgcctccag ggtgacgtcg
     6841 cggagcgcga cgcgttcacc ggatcggacg gtgaggttct ccacgcccca gctcacgtgg
     6901 agaccccctc gagcgccggc tcggcccccc ggtgccgccg gctctgcgcg ggcgccaggg
     6961 accgacggaa gcgcagggtg gccaggccga acacgaccac cccgatgatc gccaggtaga
     7021 ggaagggagc ccacagggcg gtgatcgaag cgccccggag catcacgccc cgagcgatct
     7081 ggatgaagta ggtgagcggc agcacgtagc cgatccaccg gaccccggtg gccatggagg
     7141 acagcgggaa gatcagaccg gagagcatca cctgcggcag gagggtcatc agggcgagct
     7201 ggatggcctg gccctggttc tcggagacgc tggagatcag gacgccgatc cccagcgtga
     7261 cgaacaggaa caggagggct cccagggtca ggaccaggac cgagccccgg aacggaacgt
     7321 cgaacagcca gatcccggcc accacgatca cgatcatgtc gaccgcggcc acggcgaagt
     7381 acggggcgat cttgcccagg aacacgtccg agggtcggaa cggcatcacc gcgagctgct
     7441 ccagggtccc ggcctgccgc tcgcgcacca cgcccaggct ggtgatcacc gtgccgacga
     7501 agagcaggat catggcggcc aggccgggga ccatgatcgc ggccgtctcg agcctggggt
     7561 tgaacaggac ctgcacgctc gtcgagacgc cgctggcggt ccgttcggcc agcgtgctca
     7621 gcgaggctcg agcggccctg gcgatgaaca gctcggagcc gtccaggagg accgtcacgc
     7681 cggaggaccc ggtgacgatc gccacgggca cgtcaccgtc gcggagcgcc gccaccgcct
     7741 cggcgcggcc ggcggcggga tcggtggttc ggacctcgaa gggggcggga agcgcatcgg
     7801 ccacgcgcgc cgcctgggga ccgaccacgt ccacggagat gcgctgcacg tcgaacctcg
     7861 cggcgtagcc gaacaccacc agcatcagga tcggcaggaa gatcatcatc gccagcgtcc
     7921 gccggtcgcg ccgcagctgg cggaactcct tcacgatcat cgcccacatg atggatcccc
     7981 cttgccccac cgctcggggc ctcggctcgc ccgcacggag gaattctaca cacgttgaat
     8041 tccggagggc gagtcaggga tcgggtgccg ccgccagggc gccctccacg tcatcgcgcc
     8101 ggatctcgcc gatcagctca ccgctcgagg tggtcaccgc tccacggggg aagtcgtgct
     8161 cgtccatgaa gtgggccatc tcccggagcg agacgttcgg ccggaaggtg ctgggacccg
     8221 ggagcatgac ctcaccggcg gcgcgctccg ggtcggcctc cagctccttc cccacccgga
     8281 gctgcccctg gacgatccgc cgttcggtca ccaccaggca gcggtcgaag atggtcccct
     8341 ccacccgtga gcgggccgcg gcgacggtct cgtggggcag gcaggtcggc acgtcccgcc
     8401 gggcgatccc cccgatccgg ggcagcgagg tcacctcgcc ttccgagggc agcccggcgg
     8461 cgaaccagtc ctgcttgccc ggccagtagt cgtagacctc ggagaacccg agcgcttcga
     8521 gccgccacgc ggcccgtggg ctcatgtctc aggagtagtc gtcgcagtag acgatcaccg
     8581 gagcgtcggt ccgcagtcgc tcccgagctt cgcggtcgat ccggcggagc gggatgttga
     8641 tcgcgcccgg caggtggctc tcctcgaact cttcccgccc gagcacgtcg accagctgcg
     8701 cccctcgatc gatcagttct cgcacccggt ctcgctggat gaaggtcggc atcgtgctcg
     8761 tacctttccg gtgaccgctc tcggctaacc gaagtgctcg ttacccttcc ggttgcggat
     8821 ctcggcgaac cggagtgaga tggcgtctcc ttggcggtgg ccgccgaggc caggcgcgag
     8881 gagacctatg gtgtgcttgg tgacgcatct gttcgacctg gctgaccggt tctaccgcgg
     8941 gggcatggag cgggccctct tcctgcgggc cgagcgaggt cccgacggcc gtgggacgtt
     9001 ctgcctggtc cgcggcgagc ccctgcccac cgccttcggc caggatgcct accgacgggt
     9061 gttcggctcg aaggtgggga cgccttcgcc ctcgtacagc gcggagcgat gacggaaccg
     9121 gcggtgggca ccagctctac cagagcctct accaggaccc gagcggtccg attcgtgctg
     9181 ctggtcggcg tgatgagcct gttcgccgac ttcacctacg agggctcccg cagcatcgtg
     9241 ggccagtacc tgggcacgct cggagccggt gccctcgcgg tcgccgtagt gtccgggttc
     9301 ggggagctgc tcgggtacgg gctgcggctg gtgtccggtc gggcggccga tcgcaccggc
     9361 cggtactggc cgatcaccat cggcggctac gtgctccaga tgtcggtggt gccgctgctg
     9421 gcgctggcgg ggagctggcc actggccgcc gccctggtga tcctggagcg ggtgggcaag
     9481 gccacccgga acccgccgcg cgacgcgatg ctctcccacg cggcgggtga gatgggctac
     9541 gggtgggggt tcggggtgca cgaggccctc gatcagctgg gggccgtgtt cggcccgctg
     9601 ctggtggcgc tgatcctggc cgtcggtcac cacgactacc ggctggcctt cgcctcgctg
     9661 gcgatcccgg cgctcatcat gctctcgctg ctgggcgtgg ccaggatgct gtacccgcga
     9721 ccccaggacc tggaggcggg ccccggccgg gtgagcggcg agggcctgcc gcgcgtgttc
     9781 tgggtgtacc tggccggggc ggccctggtg ggtgccgggt tcgccgactt cccgctgatc
     9841 gcgttccact tccagagcgc ccgtacggtg agcaccaccg agatcccgct gctgtacgcg
     9901 gccgcgatgg ccgtggacgg cgtg
//
LOCUS       DASQQQ010000369         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_102980, whole genome shotgun sequence.
ACCESSION   DASQQQ010000369 DASQQQ010000000
VERSION     DASQQQ010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102980"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VE646_13910"
     CDS             complement(<1..569)
                     /locus_tag="VE646_13910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZD81119.1"
                     /translation="MRFGIFYEHQLPRPWDEGDELQLIQDALDQVELADRLGIEYVWE
                     VEHHFLEEYSHSSAPEVFLAACSQRTRNIRLGHGIVQTPPPFNHPARVAERIAMLDLV
                     SGGRVEFGTGESSSEAELGGFNIDPALKREMWQEGLEVAVRCLTEAPFTGVDGTFVKM
                     PPRNVVPKPVQKPHPPLWVACSRRDTILLA"
     gene            complement(613..1710)
                     /locus_tag="VE646_13915"
     CDS             complement(613..1710)
                     /locus_tag="VE646_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81120.1"
                     /translation="MTTLQTEFTEHELLQTHPITEPLIAGGVRCHGGFAESGTYVSPR
                     TLNRVPAIRAWQELRSEQFGTPLLDIPLDRWPEHYPNVAQAKFLIREGVPQPISTTLT
                     RVGTVEGFGSMIRYSTVPDLDRCFDEDVRGTAMTHLDRGLYEAHARDEAGYEDEGGHQ
                     QMWFAARDVAFENPPTEDETNRMLERMGLRQPGGASGPPPAPPRLLPDDIDHDLELLI
                     HRMARLLLIEISAFHTFAWAEEVLSDIDLVAGEGQAARLVSYIRADETPHVEYLKTVL
                     SEMRDRTFVGASGRKYPGADMIGRVWDHAVAESLGARREQNLQVTLREIEHALDGNPR
                     GKDVLAEFHHLGSIQPKADGTWTATGEVTGY"
     gene            1772..>2276
                     /locus_tag="VE646_13920"
     CDS             1772..>2276
                     /locus_tag="VE646_13920"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZD81121.1"
                     /translation="MSTAPRQLLGRAERYESIVKGAAQAFARSGFAATSMEDVAAAAG
                     ITKLIVYRHFDSKEELYRAVLERVSDRLAEEFLGHLNRGERTGVGVRSLLTVAREDPD
                     GFVLLFRHASREPQFADYAREHTERAVHAARVWLETVFDDAVLAEWAAQAVVGMNVEA
                     VLAWLEHG"
BASE COUNT          327 a          759 c          835 g          355 t
ORIGIN      
        1 gccagcagga tggtgtcccg gcgggagcac gccacccaca ggggcgggtg gggcttctgc
       61 accggcttgg gcacgacgtt gcggggcggc atcttcacga acgtgccgtc gacgccggtg
      121 aacggtgctt cggtgaggca ccgcacggcg acctcgaggc cctcctgcca catctcccgc
      181 ttgagcgccg ggtcgatgtt gaacccgccc agctccgcct ccgacgacga ctcgccggtg
      241 ccgaactcca cccgccctcc cgacacgagg tcgagcatgg cgatgcgctc ggcgacccgg
      301 gccgggtggt tgaacggcgg cggcgtctgc acgatgccgt ggcccagccg gatgttccgg
      361 gtgcgctggc tgcacgccgc caggaagacc tcgggggcgc tcgagtgcga gtactcctcg
      421 aggaagtggt gctccacctc ccacacgtac tcgatgccga gacggtcggc gagctcgacc
      481 tgatcgagcg cgtcctggat cagctgcagc tcgtcgccct cgtcccaggg ccggggcagc
      541 tggtgctcgt agaagatgcc gaaacgcacg ggtcctcctg tcaggggtgg atcggagctg
      601 gatcagggat ggtcagtagc cggtgacctc gccggtggcg gtccacgttc cgtcggcctt
      661 gggctggatg ctgccgaggt ggtggaactc ggcgaggaca tccttccccc gggggttgcc
      721 gtcgagcgcg tgctcgatct cgcggagcgt cacctggagg ttctgctccc gccgggcgcc
      781 gagtgactcg gccaccgcgt ggtcccacac ccggccgatc atgtcggcgc ccgggtactt
      841 ccggccagac gcgcccacga aggtgcgatc acgcatctcc gacagcacgg tcttcaggta
      901 ttcgacgtgg ggcgtctcgt cggcccggat gtacgagacc agccgggccg cctgcccctc
      961 gccggccacc agatcgatgt cggacagcac ctcctcggcc caggcgaacg tgtggaaggc
     1021 cgagatctcg atgagcagca ggcgggccat ccggtggatc agcaactcca ggtcgtggtc
     1081 gatgtcgtcg ggaaggagcc gcggcggggc cgggggtggg ccggaggcgc cgcccggttg
     1141 ccggagcccc atgcgctcca gcatccggtt ggtctcgtcc tccgtgggcg ggttctcgaa
     1201 ggcgacgtcg cgggcggcga accacatctg ctggtggcca ccttcgtcct cgtagccggc
     1261 ctcgtcgcgg gcatgggcct cgtacaggcc gcgatcgagg tgtgtcatgg ccgtgccccg
     1321 cacgtcctcg tcgaagcagc ggtcgaggtc gggcacggtc gagtagcgga tcatcgaacc
     1381 gaagccttcc acggtgccga cccgggtcag cgtggtgctg atcggctggg gtacgccctc
     1441 ccggatgagg aacttcgcct gggcgacgtt ggggtagtgc tccggccagc gatcgagcgg
     1501 gatgtcgagc aggggagtgc cgaactgctc ggaccgcagc tcctgccagg cccggatggc
     1561 cggcacccgg ttgagcgtgc ggggcgacac gtacgtgccg ctctcggcga agccaccatg
     1621 gcagcggacc ccgccggcga tcaacggctc cgtgatcggg tgcgtctgca gcagctcgtg
     1681 ctcggtgaac tcggtctgga gcgtggtcac cggacaagtg taaactgcgg agtatacacc
     1741 cgacaagagc cacggaaggg aaaatgaggc cgtgagcacc gcaccccgcc agctgcttgg
     1801 tcgggcggag cgctacgagt ccatcgtgaa gggcgcggcg caggccttcg cccggtcggg
     1861 gttcgcggct acctcgatgg aggacgtggc ggcggcggcc ggcatcacca agctcatcgt
     1921 gtaccggcac ttcgactcca aggaggagct ctaccgggcc gtgctcgagc gggtctccga
     1981 ccggctggcc gaggagttcc tggggcacct caaccggggt gagcgcaccg gcgtgggcgt
     2041 gcggtcgctg ctcacggtcg cccgggagga ccccgacggg ttcgtgctcc tgttccggca
     2101 cgcgtcccgc gaaccccagt tcgccgacta cgcgcgcgag cacaccgagc gggccgtgca
     2161 cgctgcccgg gtctggctcg agaccgtgtt cgacgacgcc gtgctggccg agtgggcggc
     2221 ccaggcggtc gtgggaatga acgtcgaggc ggtgctcgcc tggctcgagc acggtg
//
LOCUS       DASQQQ010000370         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_275543, whole genome shotgun sequence.
ACCESSION   DASQQQ010000370 DASQQQ010000000
VERSION     DASQQQ010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275543"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VE646_13925"
     CDS             <1..840
                     /locus_tag="VE646_13925"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZD81122.1"
                     /translation="EPASVFRIAPTTTLVSNLLADWLQNGVGASKFIHVYEASDYGLY
                     QADSYKALLEGTPIELVQVQVELNQPDYSAVLSRLAQEHGDADVVLLDVTGESGYTTV
                     QNSFDVGLLGPDSGAICFASQEAQNDKAWWRVVPDGVGCVFTFVGPPPSAYNEHTSSV
                     ADRYREEFDGEPNVWTFEAYDSVRLVADAIERAGSTEPEAVVQALETTTFEGAQGAYS
                     FPYGSQNPLPADQPTWMWHQWPDPTVQYLEYTAPGQPLEEAAIVWPASKQTDGVAYVD
                     VSG"
     gene            898..>2016
                     /locus_tag="VE646_13930"
     CDS             898..>2016
                     /locus_tag="VE646_13930"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZD81123.1"
                     /translation="MAPVLAWLLIAGPVWALAGSVALPRRYRARALDAGLARLSGGLI
                     GAAAGLPGLALFYRRTPELRRWRDVGGPALLLAAELALLFRAAFPSNVCVTGPLYALD
                     QAQNGASVGMVYATIAVGLTIIFSVQGIVSFAHGQFFMFGGVLAYLLVSEVWEANPVY
                     AIPIAGAAALLLGAAFERTLLAPMHAGRVERVVEYAILVTFGFGMFLQYSLVGLMGSP
                     RGIKSPRYTDLPIAGIDTPAFDLGPLRVRTDFLIAGLIGLVLILGLQWFLQQSWTGMS
                     LRAVSMNRDAAAAVGINAGRAFTIAFAIGTMLAGMAGAALVPALSFAVPQIAGQAALR
                     SYVVIVLGGLGSIPGALLGGLFIGVVEALGAACFPDPSR"
BASE COUNT          259 a          694 c          730 g          333 t
ORIGIN      
        1 gagccggcaa gcgtgttccg gatcgctccg acgaccacgc tggtgagcaa cctgctcgcc
       61 gactggctcc agaacggcgt cggcgcgagc aagttcatcc acgtgtacga ggcgagcgac
      121 tacggcctgt accaggccga tagctacaag gcgctgctgg agggcacccc gatcgagctg
      181 gtgcaggtcc aggtggagct gaaccagccc gactactcgg cggtgctgag ccggctggcc
      241 caggagcacg gcgacgcgga cgtggtgctg ctggacgtca ccggggaatc gggctacacg
      301 actgtccaga actccttcga cgtgggactg ctcgggccgg acagcggcgc catctgtttc
      361 gcgagccagg aggcccagaa cgacaaggcg tggtggaggg tggtccccga cggggtgggc
      421 tgcgtcttca ccttcgtcgg ccccccgccc tcggcctaca acgagcacac cagctcggtg
      481 gccgatcgct accgggagga gttcgacggg gagcccaacg tgtggacgtt cgaggcctac
      541 gactcggtgc ggctggtggc cgacgcgatc gaacgagccg gatcgaccga gccggaggcg
      601 gtcgtccaag ccctggagac caccacgttc gagggcgcgc agggcgcgta ctcgttcccg
      661 tacggctcgc agaacccgct gcctgctgac cagccgacct ggatgtggca tcagtggccc
      721 gacccgaccg tgcagtacct ggagtacacg gcccccggcc agccgctgga ggaggccgcg
      781 atcgtgtggc ctgcctccaa gcagaccgac ggcgtcgcct acgtggatgt gagtggctag
      841 gctgagtccg ccgggtgcgg gcagcgccgg cacccccgca ccggggaggg atcgctcatg
      901 gcgcccgtcc tcgcctggct gctgatcgcc ggccccgtgt gggcgctggc gggatcggta
      961 gctctcccgc gccgctaccg cgcgcgtgcg ctcgatgccg gcctggctcg gctgtccggc
     1021 gggctgatcg gcgcggcggc cgggctcccg ggcctcgccc tcttctaccg gcgcaccccg
     1081 gagctccgcc gctggcgcga cgttgggggc ccggccctcc tgctcgctgc cgagctcgcg
     1141 ttgctgttcc gggccgcctt cccctcgaac gtgtgcgtga ccggcccgct gtacgcgctg
     1201 gatcaggccc agaacggggc gtcggtgggg atggtgtacg cgacgatcgc cgtcgggttg
     1261 accatcatct tctcggtcca ggggatcgtc agcttcgccc atggacagtt cttcatgttc
     1321 ggcggggtcc tggcctacct gctggtcagc gaggtgtggg aagccaaccc cgtctacgcc
     1381 atccccatcg ccggggccgc cgcgctcctc ctcggtgccg cgttcgagcg caccttgctg
     1441 gcgccgatgc acgctgggcg cgtggagcgg gtggtcgagt acgcgatctt ggtcaccttc
     1501 ggcttcggga tgttcctgca gtactccctg gtgggcctga tgggcagccc ccgggggatc
     1561 aagagccccc gctacaccga cctgcccatc gccgggatcg acaccccggc cttcgatctg
     1621 ggtccgctgc gggtgcgcac cgacttcctg atcgcgggcc tgatcgggct cgtgctgatc
     1681 ctcggtctgc agtggttcct ccaacagtcg tggacgggca tgagcctgcg ggcggtctcg
     1741 atgaatcggg acgccgcggc ggcggtgggg atcaacgcgg ggagggcgtt cacaatcgcg
     1801 ttcgcgatcg ggacgatgct ggccgggatg gccggcgcgg ccctggtccc agcgctcagc
     1861 ttcgccgtcc cccagatcgc cgggcaggcg gcgctccgct cttacgtggt gatcgtgctg
     1921 ggcgggcttg gctcgatccc cggcgccctg ctgggcggcc tgttcatcgg cgtggtggag
     1981 gcgctggggg cggcctgctt ccccgacccg agccgg
//
LOCUS       DASQQQ010000371        20677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_411764, whole genome shotgun sequence.
ACCESSION   DASQQQ010000371 DASQQQ010000000
VERSION     DASQQQ010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 20677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20677
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411764"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..1035
                     /locus_tag="VE646_13935"
     CDS             28..1035
                     /locus_tag="VE646_13935"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZD81124.1"
                     /translation="MRFGLALPHYDFSLPSGDPISFARMVQFARLADRVGFDSVWISD
                     HFFYSLARYGGPPDLQGSLEPLTALAGLAPLTDRVRLGTLVLCAPFRHPAVLAKSVTA
                     IDQLSGGRFDLGIGAGWYEEEFRAFGYEYGSVGERFRILEETVEILDRLLPGGPVDVD
                     GGRYTLRRAYNRPLPAQRPRPPIWIGAKGGDRALRLAARHADGWNSVWRWSPEDYARK
                     VARAHEICEREGRDPATFRLSLGLYTLVGENRDDLVARFRAMQRWMPGGALDGELLDA
                     FMVDTLTGTPEQVVERLAAFAELGVEELIVSPAPIPFAVPDPTILELFAETVIPRAKD
                     L"
     gene            1039..1248
                     /locus_tag="VE646_13940"
     CDS             1039..1248
                     /locus_tag="VE646_13940"
                     /inference="COORDINATES: protein motif:HMM:NF016924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HZD81125.1"
                     /translation="MEYLEAALAVLREEGEPLHWTVIQDLALRRGYLDPFTQRDIRKH
                     LVAALAAAARDGLLAKEGTGVYRLP"
     gene            complement(1264..2262)
                     /gene="glpX"
                     /locus_tag="VE646_13945"
     CDS             complement(1264..2262)
                     /gene="glpX"
                     /locus_tag="VE646_13945"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HZD81126.1"
                     /translation="MSAQDRPDRNLAMELVRVTEAAALAAGRWIGRGDKEAADQAAVD
                     AMRLMIDSVSMDGTVVIGEGEKDEAPMLFNGESVGNGTGPAVDVAVDPIDGTRLTAGG
                     QPNALAVIALAERSSMFFPGAAVYMEKVATGPEAADAIDVTAPPEENVRRVAKAKGER
                     PEEIGVVVLDRDRHAAVIRRLREVGARVYLITDGDVAGAIAAASTERSGIDLLIGIGG
                     TPEGVIAAAALKCLGGAMQGRLYPRNDDERNRLVDEGFDLDRILSTDDLVTGKDVFFA
                     ATGITDGSLLRGVSYDHDSATTYSLVMRSRSGTVRYIEARHRFEKLEQFSRIAYRR"
     gene            complement(2378..3370)
                     /locus_tag="VE646_13950"
     CDS             complement(2378..3370)
                     /locus_tag="VE646_13950"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZD81127.1"
                     /translation="MPWARAFVTGGSGFVGAALVHHLLDHGLEVAALSRSGRSDVDLA
                     AAGAEPIRGDVLEPAALSHAMAGCELVFHVAALVRTCRRDPSAMVRVNVEGTRNVVAA
                     AARAGVRRLVLTSSAATIGERAGEVGNEDTPHRGWYLTAYERTKHEAEEVALELGTSL
                     GVEVVVVNPASVQGPGRAEGSARLLLLAARRRSVPVIQTWLGLVDVEDCAAGHRLAAE
                     QGLPGRRYLLCGASLSTPDLLAVLEEVLGRRLRSRGLPEAMTRVGGRLGDAWVALTGR
                     GPICAELARSAAHGHRYDGSRAVRELGLAYTPLPTTLARTVAWFRDQGLLPEPR"
     gene            complement(3375..3947)
                     /gene="pgsA"
                     /locus_tag="VE646_13955"
     CDS             complement(3375..3947)
                     /gene="pgsA"
                     /locus_tag="VE646_13955"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HZD81128.1"
                     /translation="MNERVAKAASLLRRLTYVRVLLVPVVMWLVLDDPFRRAYGVAAG
                     LFAFAAATDFFDGYLARRWGATTTLGSFLDTTADKLLVSGVLVALVQIGRASPWIAAI
                     IIGRELLILGLRGVVAADGTVMRPSIWGKLKANAQFLAIFLAILRIPPELGPLFLDQW
                     VMLVAAAVTVVSAVEYLARFSGSLSPSRRS"
     gene            complement(3972..6458)
                     /gene="ptsP"
                     /locus_tag="VE646_13960"
     CDS             complement(3972..6458)
                     /gene="ptsP"
                     /locus_tag="VE646_13960"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015862197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HZD81129.1"
                     /translation="MVGIVLVSHSHRLAQGAAELAREMGGPDIHLEPVGGLDLPDHPI
                     GTDAVLVQQAIERAWSQDGVLVLMDLGSALLSAEMAIEMLPTERRERILLCEAPFVEG
                     AVAAAVAARLDRPLGEVADEARGALAAKIAHLGGTPRAEEAIAPAPEPTAQGPSATAE
                     LAVENPLGLHARPAARFVQTASSFDADVRVTNLTTGRGPASARSLNQVATLGVLQGHR
                     IGVRAVGPQAAEAVDALRELAEQRFGDEPEPPVTAATPPPPVGEGAIAGTAASPGVAV
                     GPARHFRPPEIPIPSDPVVDRERAWSRFEDALGEVRTELERTRASVASRAGDQSAAIF
                     EAHALFLQDEDLLAPVRTAVLEQGTNPARAWWDSVRAAAAGWRELDDPYLRARAADLE
                     AVGRQVLSHLVDVTPGPPVLSGPGVLLAPDLTPSDTAGLDPASVLGIGTAYGGPTSHT
                     AVLARSLGIPAAVGLGATVLQIEEGVTVVVDGDAGTLAPEPTPAEISQARTRGKRFRA
                     LEAQARLEAARPAVTRDGTRIEVVANVGRPDDAVAAAQAGAEGVGLLRTEFLFLGRTT
                     MPSEEEQEQAYRAVAEALGGRPVIVRTLDVGGDKPLPYVPMAQEANPFLGVRGLRLGL
                     ARPEILLTQLRALVRTAADHPIFVMFPMVTTLQELRSAKELLRQAGDGVRASGGATPE
                     RIPVGVMVEVPAAALQAGSFADEVDFFSIGTNDLTQYALAAERGNEHVAELADGLHPA
                     VLKLIAMTVEGAAPQERMVGVCGELAGDPVAVPILVGLGVTELSASPPAVPRVKRAVR
                     ELTLEEARALAREALSRTSAAEVRALSARP"
     gene            complement(6474..6875)
                     /locus_tag="VE646_13965"
     CDS             complement(6474..6875)
                     /locus_tag="VE646_13965"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81130.1"
                     /translation="MAVFQTRPRITRRSLYPGGRSTRVWWWTAGTGAALLVLVTVHMV
                     AHHFVVDRVGGLRTYHQVLEYIGDPAIFTIECFFLLAVTIHAMLGLRSVLFDLDMTRR
                     FRRMIDAGLSVLGVATVAYGFFLIGTLASRA"
     gene            complement(6877..7320)
                     /locus_tag="VE646_13970"
     CDS             complement(6877..7320)
                     /locus_tag="VE646_13970"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81131.1"
                     /translation="MSETITRLPGSDEREARGPVQRKGLGPAPSGWLDPRGRDIGGWA
                     FAANRVTGLGLVLYLYLHLGVLSMLLLGPSAWSGFLRLATTWAFLGLDALLLFGLLFH
                     GLNGLRVALVGSGIVPSRQKALFWAGTVIGTITLGYGIFHILGSG"
     gene            complement(7317..8108)
                     /locus_tag="VE646_13975"
     CDS             complement(7317..8108)
                     /locus_tag="VE646_13975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZD81132.1"
                     /translation="MTETMQTTTGHPSPEPAAQEPSERSAETRRFRVFRFKRGDAEPH
                     FDTFDVPIEHRTTVFEALRWIQLNRDPSLSLRHSCLHASCGTCGLQVNGREALACVTF
                     VRDLGHQITVEPIANIPVLTDLVVDMTEFYARFPDQHPYLRSSEFLPQADTPDGIGTY
                     VRFENCIECGLCLSACPIAATSDAYVGPAALAAAERLLEEPRGADLGSVLDWADRPDG
                     VWRCHAAFECTEACPSNVEPAARIMALRGELVGGRLRGGQREDRG"
     gene            complement(8116..9297)
                     /locus_tag="VE646_13980"
     CDS             complement(8116..9297)
                     /locus_tag="VE646_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012612269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZD81133.1"
                     /translation="MTLARHDAHVLIVGGGGTGGALAHDLALRGLRVTLVERGEVTSG
                     TTGRHHGLLHSGARYAVHDQESAVECIEENTILRRICPGSFEENDGLFVGVTDEDMEY
                     LEPFLKGCAESGIPTQQLSPEEALQLEPNLNPDLKVAVRIPDGTMDAMRMPLRFFATA
                     KHNGAQIRSYMEVTGLLVHGGVVSGAALRDHVTGREGEVHADLVVNATGPWSERVAAM
                     AGVDVPIQPSPGVLLALRGRLCNMVINRLHKSGDGDIIVPQRALSVVGTSSWTVEDPD
                     HLDVPEDHVRRMYEEGAKLIPAVSQAEFRAAWSAARPLIGSRGAADTGRELSRTFKTY
                     DHKETDGVEGFVTITGGKGTTLRGMAELCANVICGKLGIEEACRTRETVLLPHTAYYA
                     A"
     gene            complement(9523..11019)
                     /gene="glpK"
                     /locus_tag="VE646_13985"
     CDS             complement(9523..11019)
                     /gene="glpK"
                     /locus_tag="VE646_13985"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HZD81134.1"
                     /translation="MADFAAALDQGTTSTRFMIFDHQGKVKSIDQKEHEQIYPKPGWV
                     EHDPIEIWNRSEQVISGSLGKEGLKASDLSAVGITNQRETTVVWDRSTGKPVYNAIVW
                     QDTRTDQICNQLAADGGQDRFRPKTGLPIATYFSGPKIKWILENVDGARAKAEAGELM
                     FGNIDTWCIWNLTGGVNGGVHVTDVTNASRTLMMNLETLDWDEEILGILGVPRSMLPE
                     IKPSSGVYGDATGDLAGIPVAGDLGDQQAALFGQTCFAVGEAKNTYGTGCFLLLNTGT
                     KPVPSKSGLITGLGYKIGDQPAVYMLEGSIAITGALVQWLRDNLGMIQSSAEVEALAA
                     TVDDNGGVYFVPAFSGLFAPYWKSDARGVIAGLTRYVNKGHIARAVLEATAWQTREVA
                     DAMKVDSGVELSSLKVDGGMVYNELLMQFQADVLGVPVIRPTVAETTALGAAYAAGLA
                     VGFWKEVEDLRANWGKDKEWDPKMDPAERDKEYGFWKKAVTRTFDWVE"
     gene            complement(11043..11687)
                     /gene="dhaL"
                     /locus_tag="VE646_13990"
     CDS             complement(11043..11687)
                     /gene="dhaL"
                     /locus_tag="VE646_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017316502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HZD81135.1"
                     /translation="MVSVPYDLVVRWVQRFAEVIAANKEELTALDAAIGDADHGINMD
                     RGMRAAMAKLDGVPAGDVGGLLKAVGMTLVSTVGGAGGPLYGTLFMQMGVALAGKSEL
                     APQDWAEALSKGLDGVVMRGKAELDDKTMVDALTPARDALKEALDEGASFGDALRRSA
                     SAAEEGMKKTIPLVARKGRASYLGERSAGHQDPGATSSFLLLEATVETWADADP"
     gene            complement(11737..12738)
                     /gene="dhaK"
                     /locus_tag="VE646_13995"
     CDS             complement(11737..12738)
                     /gene="dhaK"
                     /locus_tag="VE646_13995"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HZD81136.1"
                     /translation="MKKLINKPDDVVTEELQGVAAAHPDLVRVNFGPNYVVRADAPVQ
                     GKVALISGGGSGHEPMHGGFVGMGMLDAACPGEVFTSPTPDQMYEAAKASHGGAGVLF
                     IVKNYTGDVMNFDMAAELARGDGMEVESVLVDDDVAVQDSLYTAGRRGVGTTVLMEKI
                     AGAAAEEKRPLKDVADIARRVNENGRSMGMALTSCTVPAAGKPTFELGDDEFELGIGI
                     HGEPGRERMKMKPVSEIVEMMSTAVVEDVPFQSGDKVLAFVNSMGGTPLIELYVVYNE
                     LKRFLDGRGISIERKLIGPYITSLEMQGCSITLLKLDDELTKLWDAPVKTAGLRWGV"
     gene            complement(12815..13552)
                     /locus_tag="VE646_14000"
     CDS             complement(12815..13552)
                     /locus_tag="VE646_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HZD81137.1"
                     /translation="MLIGIGVLILLGDGVVAGVLLNRSKAQNSGWIVITFGWGMAVLF
                     GVWTSVAMGGQGDINPAVTIAKWASSRALFDPAVGGGFTQMVLQVAGEFVGAFAGAVL
                     VYLAYINHWSETEDPGLKLAVFSTGPAVRNYAANFITEVIGTAMLLFGVFGIFVAGGG
                     TSVAGTTLALNVFWVALLVFSIGMSLGGPTGYAINPARDLGPRIIHAILPIPGKGTSE
                     WEYSWVPVLGPIVGGILGAWLARAVFL"
     gene            14021..14245
                     /locus_tag="VE646_14005"
                     /pseudo
     CDS             14021..14245
                     /locus_tag="VE646_14005"
                     /inference="COORDINATES: protein motif:HMM:NF014754.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
     gene            14394..14558
                     /locus_tag="VE646_14010"
     CDS             14394..14558
                     /locus_tag="VE646_14010"
                     /inference="COORDINATES: protein motif:HMM:NF014754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HZD81138.1"
                     /translation="MRATVPMQARKGRASYLGPRSVGHQDPGATSAALLFSALERAAD
                     HDGCGGSARP"
     gene            14567..15322
                     /locus_tag="VE646_14015"
     CDS             14567..15322
                     /locus_tag="VE646_14015"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZD81139.1"
                     /translation="MLRAARILQELGAGSPRLGVTELAERLGLAKATVYGLLRSLEAE
                     EFVERDGETGKYRLGPALLRLGNAFLDNHELRARSLLWAESLAGRLVEAVRVGVLNGD
                     SVLIVHHVFRPDNSVQILEVGASIPWNACALGKAIVAHVGPTRREELLAGELPQLTGR
                     TIVDRGELGATLDRVASEGFATEDQEGIVGEAEVAAPVFEHLGNPVGAIGVAGPVERL
                     LQDGPSKEVIATVREVARSLSRDMGAGRLARAR"
     gene            15337..16221
                     /locus_tag="VE646_14020"
     CDS             15337..16221
                     /locus_tag="VE646_14020"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZD81140.1"
                     /translation="MPARRLGLLALGVVAVSFSAIFIRWAAAPALAVAFYRNAMAAAV
                     LLPIALARHREEFRRLSRRDWAIALSAGGLLALHFGLWIPSLSYTTVAASTVLVTSQP
                     VWVAVMGRGMGESASGATLGGIALSMLGVAIISGGDLGLSGRAGLGDLLALLAAVAAA
                     GYFLSGRTLRARLSLLTYVGIVYTTCSVLLAATMAVAGTPFSGFSLRVWGLFALMALV
                     PQFLGHTVFNYLLAHLRATVVAVAVTGEPVGATLLAFVILGEVPRWTAFAGGAVILAG
                     IYVTLSAQTRRPAPVPIE"
     gene            16302..17138
                     /locus_tag="VE646_14025"
     CDS             16302..17138
                     /locus_tag="VE646_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZD81141.1"
                     /translation="MSGIDLHSHTTRSDGTFTPTEVVNLAAELGLEMVGVTDHDTTDG
                     LGEAAARGLAVGVQVIPGVEFSTEYQGTSVHVLCYWMDPANEEFRAELRRLIDSRFRR
                     GELMVEKLRALGYPISFQRVRQISGGGNIVRPHVARAMVEAGIVKDEQEAFDRFIADG
                     GPAAVPKHALDPVDAVGLIRRAGGVCVLAHPGMWGDQTEVPTELIEAMTAVGMAGLEV
                     DHTDHTPEQRARFRALAGRLGLIPTGGSDCHGTRYNPVRLGTVTTDPEALAALRGLVT
                     AS"
     gene            complement(17131..17721)
                     /locus_tag="VE646_14030"
     CDS             complement(17131..17721)
                     /locus_tag="VE646_14030"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZD81142.1"
                     /translation="MRETVHFEEPRVARWLFVSPAAAWIWLVIRVYLGYEWASSGWDK
                     IFGSPSWITSSASLKGFAAGALQGAAQGPHSAVNYGWYAAFLRWISSDGAGILAPLIA
                     IAEFTFGIALILGVVTGIFAFLAGLLTMSFGLAGVAGVNPVFFVFEVLLILAWRNAGY
                     IGLDRWVLPAVGTPWEPGPVLRRIRLPERERERGLS"
     gene            17843..18505
                     /locus_tag="VE646_14035"
     CDS             17843..18505
                     /locus_tag="VE646_14035"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017972724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZD81143.1"
                     /translation="MGALEMVFLDIGGVLYGDAGYREALRRALRELGARFSDQEFDAE
                     YARCRRDQSGSFRRRLTRRFLGPDADLDAVERHAGAWWHYTPEDLEPDALPTVRELAR
                     SYRLGLVANQPSGVRRALARDGLEPYFEVRAVSDDVRLEKPDPRIFVHALASAGVRGD
                     RAVMVGDRLDYDVRPAREAGMRAVWLLRGEAPDDPTPEQLAEPDAAIRSLRELREVLE
                     EL"
     gene            18502..19806
                     /locus_tag="VE646_14040"
     CDS             18502..19806
                     /locus_tag="VE646_14040"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZD81144.1"
                     /translation="MNGKRVLVVGGGYIGMYAALRMERPLDEDGHELVLVNPENFMTY
                     QPFLPEVASGNIEPRHVLVPLRQVLRRTRVIVGEVEQVDHPRRTATIRTSDGQRMELP
                     YDVVVLGAGSRSRVLPVPGLARHGIGFKTVPEAIYLRNRLLSRLDAAVTAPDEASRLR
                     ALTFVFVGAGYAGVEAMAELEDLARSAVRYYREISREDMRWVLVEAADHILPEIGTDL
                     AEHVRRELEGRGIEVLLGTQLKSAEAGVIRLSDGQSFPADTLVWTAGVRPEPLGDRSG
                     FPVDASGRIEVDAYLRVKGVSDAWAAGDAAAVPDLVLGGITPPTAQHAVRQGRRLGEN
                     LVASLRGQPPRPFRWHNLGQLASLGRYKGAAMVLGVRLRGFPAWWLHRSYHLAYMPTL
                     NRKVRIVADWTVGLLFPRDVVQLGSLQRPREAFRRAAGRERD"
     gene            complement(19803..20276)
                     /locus_tag="VE646_14045"
     CDS             complement(19803..20276)
                     /locus_tag="VE646_14045"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZD81145.1"
                     /translation="MQVGDIMHTDVKTANPEDAFADVAKIMQANGISSVVVLDDHHLA
                     GILTERDVVNLVAAGADPHTVTVKMGMTRRHLETVTPRTDVVEAAELMVARNIRHLPV
                     VDGEEVVGIVSIRDLTRWAADELSGGHELPDIERSHAALKAASELQRKRSAQPLA"
     gene            complement(20350..>20677)
                     /locus_tag="VE646_14050"
     CDS             complement(20350..>20677)
                     /locus_tag="VE646_14050"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HZD81146.1"
                     /translation="EPGPASLQDMIAAVDRGVLVTRFHYSNVVNPMESSITGMTRDGT
                     FWVEDGQVRHPVKNLRFTQSIVEALSHTVAIGRETELASEFFFSASRVPALVVDGFNF
                     TGRSDH"
BASE COUNT         2904 a         7432 c         7245 g         3096 t
ORIGIN      
        1 ccggccgacg tgcttcggta cctgtagatg cgtttcggcc tggcgctgcc gcactacgac
       61 ttctccctcc cctcgggcga ccccatctcg ttcgcccgga tggtgcagtt cgcccgactg
      121 gcggatcgcg tcgggttcga ctcggtgtgg atctcggacc acttcttcta ctcgctggcc
      181 cggtacgggg gcccgcccga cctgcagggg tcgctggagc cgctcacggc gctggccggc
      241 ctggccccgc tgaccgaccg ggtgcgcctg ggcacgctcg tgctgtgtgc gccgttccgc
      301 catcccgcgg tcctggccaa gagcgtgacc gcgatcgacc agctctcggg cggccggttc
      361 gacctgggga tcggtgcggg atggtacgag gaggagttcc gggcgttcgg ctacgagtac
      421 gggtcggtgg gggagcggtt ccggatcctg gaggagacgg tcgagatcct ggaccgactc
      481 ctgcccggcg ggccggtgga cgtggatggc ggccggtaca cgctccgtcg ggcgtacaac
      541 cgaccgctgc ccgcccagcg gccccgtccg ccgatctgga tcggggccaa gggcggcgac
      601 cgtgcgctcc ggctggcggc ccgccatgcc gacggctgga acagcgtatg gcgatggtcg
      661 cccgaggatt acgcgcggaa ggtggctcgg gcgcacgaga tctgcgagcg cgaggggcgc
      721 gacccggcca cgttccggct ctcgctgggt ctctacacgc tcgtcggcga gaaccgcgac
      781 gacctggtgg ccaggttccg ggcgatgcag cgatggatgc cgggaggtgc tctcgacggc
      841 gagctgctgg acgcgttcat ggtggatacc ctcaccggga cgccggagca ggtggtggag
      901 cggttggcgg ccttcgccga gctgggggtg gaggagctga tcgtgtcgcc cgcgccgatc
      961 cccttcgccg ttcccgaccc gacgatcctc gagctgttcg ccgagacggt gatccccagg
     1021 gccaaggacc tgtagggggt ggagtacctt gaggcggccc tggcggtcct ccgggaggag
     1081 ggggagcccc tgcactggac cgtgatccag gatctggccc tccgtcgggg gtacctggac
     1141 ccgttcaccc agcgcgacat ccgcaagcac ctggtggcgg cgctggccgc ggccgcccgc
     1201 gatggtctgc tggccaagga ggggaccggc gtctatcggc tcccctgagg gacctgcggg
     1261 gccctaacga cggtaggcga tccgggagaa ctgctccagc ttctcgaacc ggtggcgggc
     1321 ctcgatgtat cggacggtcc cggagcggga gcgcatcacc agcgagtagg tggtggcgct
     1381 gtcgtggtcg tagctcaccc cgcgcaggag cgaaccgtcg gtgatcccgg tggccgcgaa
     1441 gaacacgtcc ttgccggtga ccaggtcgtc ggtggacagg atccggtcca ggtcgaagcc
     1501 ctcgtccacc agccggttcc gctcgtcgtc gttgcgaggg tacaggcgtc cctgcatcgc
     1561 gccgcccagg cacttcaggg cggcggcggc gatgacgccc tcgggggtcc ccccgatccc
     1621 gatcaacagg tcgatccccg agcgctccgt gctggccgcg gcgatggccc cggccacgtc
     1681 accgtccgtg atgaggtaga cccgggctcc gacctcccgc agacgccgga tcacggccgc
     1741 gtgccggtcg cggtccagga ccaccacgcc gatctcctcg gggcgctccc ccttggcctt
     1801 cgccacccgg cggacgttct cctcgggcgg tgcggtgaca tcgatcgcat cggcggcctc
     1861 gggaccggtg gccaccttct ccatgtacac cgcggctccc gggaagaaca tcgagctccg
     1921 ctcggcgagc gcgatcaccg cgagggcgtt cggttgaccg ccggcggtga ggcgggtgcc
     1981 gtcgatcgga tccaccgcca catccaccgc ggggccggtg ccgttgccca cggactcacc
     2041 gttgaagagc atcggggcct cgtccttctc gccctctccg atcaccaccg tcccgtccat
     2101 cgacacgctg tcgatcatca gacgcatcgc gtcgaccgcg gcctggtccg cggcttcctt
     2161 gtctccccgt ccgatccagc ggccggcggc cagcgcggcg gcttcggtga cgcgcacgag
     2221 ctccatggcg aggttgcgat cgggtcgatc ctgggcgctc atagacggct tcctccgtga
     2281 ggttcagggg ccccggcctc tggggaggcc gttccggcca tcgtacgccg gtcgctcccg
     2341 gcccgcccgg ccggcggagc gccggggcgc gcgatcgcta gcggggctcg gggagcaggc
     2401 cctggtctcg gaaccacgcc acggttcggg ccagcgtcgt gggcagcggg gtgtaggcga
     2461 gcccgagctc gcgcacggcc cgcgagccgt cgtaccgatg tccgtgggcg gccgagcgcg
     2521 ccagctcagc gcagatcggc ccccgccccg tgagggcgac ccaggcgtcc cccaaccggc
     2581 caccgacccg cgtcatcgcc tcggggaggc cccgggaacg cagcctgcgc cccagcacct
     2641 cctcgagcac ggcgagcagg tcaggggtag agaggctggc tccgcacagc aggtacctgc
     2701 ggccggggag gccctgctcg gcggccagcc ggtgcccggc cgcgcagtcc tcgacgtcca
     2761 ccagacccag ccacgtctgg atcacgggga cggatcgccg ccgtgccgcc agcagcaaca
     2821 gtcgcgccga cccctcggcc cgaccgggac cctggaccga cgccgggttc accaccacca
     2881 cctccacgcc cagggacgtg ccgagctcga gggccacctc ctcggcctcg tgcttcgtcc
     2941 gctcgtaggc ggtgaggtac caaccccggt gcggcgtgtc ctcgttcccc acctcgccgg
     3001 cacgctcccc gatcgtggcc gccgatgacg tcaggaccag gcggcgcacc cccgcacgcg
     3061 ccgccgccgc cacgacgttc ctggtcccct ccacgttcac cctgaccatc gccgatggat
     3121 ctcggcgaca cgtccggacc agtgcagcaa cgtggaacac cagctcacac ccggccatgg
     3181 cgtgggacag agcggccggc tccagcacgt ccccgcggat cggctcggcg ccggccgcgg
     3241 ccagatccac atcggatcgt ccgctcctgg agagtgcggc cacctccagg ccatggtcga
     3301 gcaggtggtg caccagcgcc gcacccacga acccagagcc gcccgtgacg aaggcgcgag
     3361 cccagggcat gccgctacga gcgccggctg ggcgacaggc tgcccgagaa cctggccagg
     3421 tactcgaccg cggacaccac ggtcaccgcc gctgccacca gcatgaccca ctgatccagg
     3481 aacagcggtc ccagctcggg cgggatccgc aggatcgcca ggaagatcgc caggaactgg
     3541 gcgttggcct tcagcttgcc ccagatcgac ggtcgcatca ccgtgccgtc ggccgccacc
     3601 acgccacgca gccccaggat caggagctcc cggccgatga tgatggcggc gatccagggc
     3661 gaagcccgcc ctatctgcac cagcgccacc aggacgccgg agacgagcag cttgtcggcc
     3721 gtggtgtcca ggaagctgcc gagggtggtg gtggcgcccc accgcctggc caggtagccg
     3781 tcgaagaagt cggtggccgc cgcgaaggcg aacagcccgg ccgccactcc gtacgcacgc
     3841 cggaacggat cgtccagcac gagccacatc accaccggga ccaggagcac ccggacgtac
     3901 gtgagccggc ggaggagcga cgccgctttc gccacccgtt cgttcacggc cggcacaccc
     3961 tagcagcggc ctcaggggcg cgccgagagg gcccgcacct cggcggcgga ggtacggctc
     4021 agggcctcgc gtgccagcgc ccgcgcttcc tcgagggtga gctcccggac cgcccgtttc
     4081 acccggggga ccgccggcgg gctggcggac agctcggtca ccccgagacc caccaggatg
     4141 ggcacggcca cggggtcgcc ggcgagctcg ccgcacacgc ccaccatgcg ttcctgcggg
     4201 gccgcgccct ccaccgtcat ggcgatcagc ttcagcacgg cggggtgcag gccatcggcg
     4261 agctcggcca cgtgctcgtt gccgcgctcc gcggccaacg cgtactgcgt gaggtcgttc
     4321 gtgccgatcg agaagaagtc cacctcgtcg gcgaaggaac cggcctgcag ggccgcggcg
     4381 gggacctcca ccatgacccc taccgggatg cgctccggcg tggccccgcc ggacgcccgt
     4441 accccgtccc ccgcctgccg gagcagttcc ttggcggacc tgagctcctg gagcgtcgtc
     4501 accatgggga acatcacgaa gatcggatgg tcggcggccg tgcgcaccaa tgcccggagc
     4561 tgggtcagca ggatctcggg tctggccagg cccaggcgga gcccgcggac ccccaggaac
     4621 gggttggcct cctgggccat cggcacgtag ggcagcggct tgtcgccgcc cacgtcgagg
     4681 gtccgcacga tcaccggtcg gcccccgagg gcctcggcca cggcccggta cgcctgctcc
     4741 tgctcctcct ccgagggcat cgtggtccgc cccaggaaca ggaactcggt ccgcagcaga
     4801 ccgaccccct ccgcgccggc ctgcgccgcg gccaccgcgt cgtcgggtcg tcccacgttg
     4861 gccacgacct cgatgcgggt gccgtcgcgg gtcaccgcgg gccgggcggc ctcgaggcgg
     4921 gcctgtgcct ccagcgcgcg gaagcgcttg ccccgggtcc gcgcctgcga gatctcggcc
     4981 ggggtcggct cgggcgcgag ggttccggcg tcgccgtcca cgaccaccgt gaccccctcc
     5041 tcgatctgca gcaccgtcgc tcccaggccc accgcggcgg ggatccccag ggacctggcc
     5101 aggacggcgg tgtgggacgt gggaccgccg tacgcggtgc cgatgcccag gaccgacgcc
     5161 gggtccagcc ccgcggtgtc cgaaggggtg agatcgggcg ccagtagcac cccgggcccc
     5221 gacaggaccg gcggccccgg ggtgacgtcg accagatgac tcagcacctg ccgtcccacc
     5281 gcctccaggt ccgccgcgcg ggcgcgcagg tagggatcgt ccagctcccg ccagccggcg
     5341 gcggcggccc ggacgctgtc ccaccaggcc cgagccgggt tcgtgccctg ctccagcacc
     5401 gccgtacgga ccggcgcgag caggtcctcg tcctgcagga acaacgcatg ggcctcgaag
     5461 atcgcggcgc tctgatcgcc ggcccgggat gccacggagg cccgggtccg ctcgagctcg
     5521 gtccggacct cacccagcgc atcctcgaac ctcgaccagg cccgctcccg gtccacgacc
     5581 ggatccgagg ggatcgggat ctctgggggc cggaaatggc gcgccggacc gaccgccacc
     5641 cccggggagg cagcggtgcc ggcgatcgcc ccctccccga ccgggggagg cggggtcgcg
     5701 gcggtgaccg gcggttccgg ttcgtccccg aaccgctgct ctgccagctc gcggagggca
     5761 tcgaccgcct cggccgcctg cggaccaacg gcacggacgc cgatccgatg gccctggagg
     5821 acgcccagcg tggcgacctg gttcaggctc ctcgcgctgg ccggaccgcg cccggtggtg
     5881 aggttggtca cccgcacgtc ggcgtcgaag gaggaggccg tctggacgaa gcgggccgcg
     5941 gggcgagcgt gaagcccgag cgggttctcc acggcgagct ccgcggtcgc ggatggtccc
     6001 tgggcggtcg gctccggcgc cggagcgatc gcctcctccg cccggggagt acccccgaga
     6061 tgcgcgatct tcgcggccaa ggccccgcgt gcctcgtcgg ccacctcgcc cagcggacgg
     6121 tccagccggg ccgccacggc cgccgccacc gcgccctcca cgaacggggc ctcgcacagg
     6181 aggatccgct ctcgccgctc ggtcggcagc atctcgatcg ccatctccgc cgacagcagc
     6241 gcgctgccca ggtccatcag cacgagcacc ccgtcctggg accaggctcg ttcgatggcc
     6301 tgctggacca ggaccgcgtc cgtgccgatc ggatgatcgg gcaggtcgag ccctccgacc
     6361 ggctccaggt ggatgtctgg gccacccatc tccctggcca gctcggccgc gccctgggcc
     6421 aaccgatggc tgtgggagac gaggacgatc ccgaccacgg ctgctcggct gcctcaggcc
     6481 cgggacgcga gcgtgccgat caggaagaag ccgtaggcga ccgtggccac gccgaggacc
     6541 gagagcccgg catcgatcat cctgcggaag cgccgggtca tgtccaggtc gaacaggacg
     6601 ctgcgcagcc cgagcatggc gtggatcgtg accgcgagca ggaagaagca ctcgatcgtg
     6661 aagatggccg ggtccccgat gtactcgagc acctgatggt aggtccgcag ccccccgacc
     6721 cggtccacca cgaagtgatg ggccaccatg tgcacggtga ccagcaccag gagcgccgcc
     6781 ccggtgcccg ccgtccacca ccacacgcgg gtcgagcgac cgcccgggta cagcgagcgc
     6841 ctggtgatcc tgggccgcgt ctggaagacc gccatctcaa ccgctcccca ggatgtggaa
     6901 gatcccgtag ccgagcgtga tcgtcccgat caccgtcccg gcccagaaca gggccttctg
     6961 gcggctcggc acgatcccgc tcccgaccag cgccacccgg agaccgttca atccgtggaa
     7021 gagcaggccg aagaggagca gggcgtccag gcccaggaac gcccacgtgg tggccaggcg
     7081 caggaacccg ctccaggccg agggcccgag cagcagcatc gacagcacgc cgaggtgcag
     7141 gtacaggtag aggaccaggc ccagtccggt gacccggttg gccgcgaagg cccagccccc
     7201 gatgtcccgc ccgcgcgggt ccagccaccc actgggtgcg gggccgaggc ctttgcgctg
     7261 caccggtcca cgggcctcgc gctcatcgga cccgggcaga cgcgtgatcg tctcgctcat
     7321 ccacgatcct ccctctggcc cccacgcagc ctcccgccga ccagctcgcc tcggagggcc
     7381 atgatgcggg cggccggctc gacgttggag ggacacgcct cggtgcattc gaacgctgcg
     7441 tggcaccgcc acacgccgtc gggccgatcg gcccagtcca gcacggagcc gaggtcggcg
     7501 ccccggggct cctccagcag ccgctcggcg gcggccagcg ctgccggccc cacgtacgcg
     7561 tcggaggtgg cggcgatggg gcaagcggac aggcagagcc cgcactcgat gcagttctcg
     7621 aaccgcacgt aggtcccgat gccgtcgggc gtgtcggcct gaggcaggaa ctcgctgctc
     7681 cgcaggtagg ggtgctggtc ggggaagcgg gcgtagaact cggtcatgtc cacgaccaga
     7741 tcggtcagga ccgggatgtt cgcgatgggc tccaccgtga tctggtgtcc gagatcccgg
     7801 acgaacgtca cgcaggccag cgcctcccgg ccgttcacct gcaggccgca ggtgccgcac
     7861 gaggcgtgca ggcaggagtg gcggagggac agcgagggat cccggttgag ctggatccat
     7921 cggagcgcct cgaacacggt ggtccggtgc tcgatgggca cgtcgaacgt gtcgaagtgc
     7981 ggctccgcat cgccccgctt gaagcggaac acccggaacc ggcgggtctc agccgagcgc
     8041 tccgagggct cctgggcggc gggctcgggg ctcgggtgcc cggtcgtggt ctgcatcgtc
     8101 tcggtcatgc ctcactcatg cggcgtagta ggcggtgtgg gggagcagca cggtctcccg
     8161 cgtgcggcag gcctcctcga tcccgagctt gccgcagatc acgttcgcgc acagctcggc
     8221 catgcctcgg agcgtggtgc ccttgccgcc cgtgatggtg acgaacccct ccaccccgtc
     8281 ggtctccttg tggtcgtagg tcttgaacgt ccgcgagagc tcgcgcccgg tgtccgcggc
     8341 gccccgcgag ccgatgaggg ggcgggcggc ggaccaggcg gctcggaact cggcctgcga
     8401 caccgcgggg atcagcttgg ccccctcctc gtacatccgg cgtacgtggt cctcggggac
     8461 gtcgaggtga tcggggtcct cgacggtcca ggagctggtt cccaccaccg acagcgctcg
     8521 ctgcgggacg atgatgtcgc cgtcgccgga cttgtgcagg cggttgatga ccatgttgca
     8581 gagccggccc cgcagcgcca ggagcacgcc gggcgagggt tggatcggga cgtccacccc
     8641 cgccatggcc gcgaccctct ccgaccacgg accggtagcg ttcaccacca ggtcggcgtg
     8701 gacctcgccc tccctaccgg tcacgtggtc gcggagggcg gcgccgctca ccaccccgcc
     8761 gtggaccagc agcccggtga cctccatgta ggagcggatc tgggccccgt tgtgcttcgc
     8821 cgtggcgaag aaccgtaggg gcatacgcat cgcgtccatc gtgccgtcgg ggatccgcac
     8881 cgccaccttg agatccgggt tcaggttcgg ctccagctgg agggcttcct cggggctcag
     8941 ctgctgggtg gggatcccgc tctccgcaca gcccttgagg aagggctcga gatactccat
     9001 gtcctcgtcg gtcaccccga cgaagagccc gtcgttctcc tcgaacgacc cgggacagat
     9061 ccggcgcagg atcgtgttct cctcgatgca ctcgaccgcc gattcctgat cgtggacggc
     9121 gtatcgggcg ccgctgtgca gcagcccgtg gtggcgaccg gtggtccccg acgtgacctc
     9181 gccccgctcg accagcgtga cacggagccc ccggagggcg aggtcgtggg ccagggcacc
     9241 gccggtgcct cctccaccca cgatgaggac gtgcgcgtcg tggcgggcaa gggtcatgac
     9301 gatccgatca cccctggggc tcccgggacc gtcccggcct cttcctcagt gctccttctc
     9361 ctcctgcccc cgggcgaagt cggggtgcgc ctcgtgctcc tccttctcct cctggcctct
     9421 ggcgaagtcc ggatgcggtt cgtgctcctt gtccatcgcc ccttccctcc tcggtccgac
     9481 cgggggggct ggcagcgccg gcccccccgg tgccgaccgg tctcactcca cccagtcgaa
     9541 ggtgcgggtg acggccttct tccagaaccc gtactccttg tcgcgctccg ccgggtccat
     9601 cttggggtcc cactccttgt ccttgcccca gttggcccgg aggtcttcga cctccttcca
     9661 gaacccgacc gcgagcccgg ccgcgtacgc ggctccgagg gcggtggtct ccgctacggt
     9721 cggccggatc accggcacgc cgaggacgtc ggcctggaac tgcatcagca gttcgttgta
     9781 gaccatcccg ccgtcgacct tcagcgagct gagctccacg cccgagtcca ctttcatggc
     9841 gtcggccact tcccgcgtct gccacgccgt ggcctccagg acggcccggg cgatgtggcc
     9901 cttgttcacg taccgggtga ggcccgcgat cacgccgcgc gcgtcgctct tccagtacgg
     9961 ggcgaacagg cccgagaacg ccgggacgaa gtacacgccg ccgttgtcgt ccacggtggc
    10021 cgccagcgcc tcgacctcgg ccgaggactg gatcatgccc aggttgtcac ggagccactg
    10081 cacgagggcg ccggtgatgg cgatggaacc ctcgagcatg tacacggccg gctggtcccc
    10141 gatcttgtag cccaggccgg tgatcagacc gctcttgctg gggaccggct tggtccccgt
    10201 gttgagcagg aggaagcatc ccgtgccgta tgtgttcttg gcctcgccga ccgcgaagca
    10261 ggtctggccg aacagggcag cctgctggtc gcccaggtcg ccggcgaccg ggatcccggc
    10321 cagatcgccg gtggcgtccc cgtagacgcc actggacggc ttgatctcgg gcagcatcga
    10381 cctcggtacg ccgaggatgc ccaggatctc ctcgtcccag tccagcgtct ccaggttcat
    10441 catcagcgtc cggctggcgt tggtgacgtc tgtgacgtgc acgccgccgt tcaccccacc
    10501 cgtgaggttc cagatgcacc aggtgtcgat gttcccgaac atcagctcgc cggcctcggc
    10561 cttggcccgg gcgccgtcca cgttctccag gatccacttg atcttcggtc cggagaagta
    10621 cgtggcgatc ggcaatccgg tcttgggccg gaaccggtcc tggccgccat cggccgccag
    10681 ctggttgcag atctggtccg tccgggtgtc ctgccagacg atcgcgttgt acaccggctt
    10741 gccggtggag cggtcccaga ccaccgtcgt ctcacgctgg ttcgtgatcc cgacggccga
    10801 cagatcgctc gccttcagcc cctccttgcc gagcgaacca gagatcacct gctcgctgcg
    10861 gttccagatc tcgatcgggt cgtgctcgac ccatcccggc ttgggataga tctgctcgtg
    10921 ctccttctga tcgatcgact tcaccttgcc ctggtgatcg aagatcatga accgggtgct
    10981 ggtggttccc tggtccagtg cggctgcgaa atcagccatg gggcctcctt ccgttgtgcc
    11041 gactacggat cggcgtccgc ccaggtctcg acggtcgcct cgagcagcag gaacgaggat
    11101 gtcgcgcccg ggtcctggtg gccggcgctg cgttcgccga ggtagctggc ccgccccttg
    11161 cgcgccacca gcgggatcgt cttcttcatc ccttcctcgg ccgccgacgc cgagcgccgc
    11221 agcgcatcgc cgaaggaggc cccctcgtcg agggcctcct tcagtgcgtc gcgggccggc
    11281 gtcagcgcgt cgaccatcgt cttgtcgtcc agctcggcct tgccccgcat gaccacgccg
    11341 tcgagcccct tgctcagggc ctcggcccag tcctgcggcg cgagctccga cttgccggcc
    11401 agcgccaccc ccatctgcat gaacagcgtc ccgtacaggg ggccgcccgc cccgccgacg
    11461 gtggagacca gcgtcatccc caccgccttg aggagcccgc cgacgtcccc tgccgggacg
    11521 ccgtcgagct tggccatggc ggccctcatc ccccggtcca tgttgatgcc gtgatccgcg
    11581 tccccgatcg ccgcgtcgag cgcggtgagc tcctccttgt tcgccgcgat cacctccgcg
    11641 aaccgctgca cccatcggac caccaggtcg tacgggacgc tcaccatccc tccttccagg
    11701 accgccggat cacgggggtg gtcaccggcg atctcctcac acgccccagc ggagcccggc
    11761 cgtcttgacc ggcgcgtccc agagcttcgt gagttcgtcg tccagcttca ggagcgtgat
    11821 ggagcagccc tgcatctcca gggacgtgat gtacgggccg atcagcttcc gttcgatgct
    11881 gatgccgcgg ccgtcgagga acctcttgag ctcgttgtag acgacgtaga gctcgatgag
    11941 cggggtgccg cccatgctgt tcacgaaggc gagcaccttg tccccgctct ggaaggggac
    12001 atcctcgacc acggccgtgg acatcatctc cacgatctcc gagacgggct tcatcttcat
    12061 gcgctcccgt cccggttcac cgtggatccc gatccccagc tcgaactcgt cgtccccgag
    12121 ctcgaacgtg ggcttgcctg ccgccggtac ggtgcaggac gtgagcgcca tacccatgct
    12181 acgcccgttc tcgttgacgc gccgggcgat gtccgccacg tccttcagcg gccgcttctc
    12241 ctcggcggcc gccccggcga tcttctccat gagcaccgtc gtgcccacgc cgcgcctgcc
    12301 ggccgtatag aggctgtcct ggaccgcgac gtcgtcgtcc accaggaccg actccacctc
    12361 catgccgtct ccccgggcca gctcggccgc catgtcgaag ttcatgacgt cgccggtgta
    12421 gttcttcacg atgaacagca caccggcgcc gccatggctc gccttggccg cctcgtacat
    12481 ctgatccggg gtgggcgagg tgaacacctc tcccgggcag gcggcgtcga gcatgcccat
    12541 gccgacgaac ccgccgtgca tgggctcgtg gcccgagccg ccgccggaga tcagggcgac
    12601 cttaccctgc accggcgcgt cggcccgcac gacgtagttg ggcccgaagt tgacccggac
    12661 caggtcgggg tgggccgccg cgacgccttg aagctcctcg gtgacgacgt catcgggctt
    12721 gttgatcagc ttcttcacat cggctccttt cctcgcagat gtgtgctgct gacccccctc
    12781 gttgttcgat ccccgatagg gataccgccc tcgcctacag gaacaccgcc ctcgccagcc
    12841 aggcgcccaa gatgccaccg acgatcgggc cgagcaccgg cacccacgag tactcccact
    12901 cggacgtccc cttcccgggg atcgggagga tcgcgtggat gatgcgcggt cccaggtcgc
    12961 gggccgggtt gatcgcgtac ccggtggggc caccgagcga catcccgatc gagaacacga
    13021 gcagcgcgac ccagaacacg ttcagggcaa gcgtggtgcc tgccaccgac gtcccgccac
    13081 cagcaacgaa gatgccgaag acaccgaaca gcagcatcgc ggtgccgatg acctcggtga
    13141 tgaagttggc ggcatagttc cggaccgccg ggccggtgct gaacaccgcc agcttcaggc
    13201 ccggatcttc ggtctccgac cagtggttga tgtaggcgag gtacaccagg accgcgccgg
    13261 cgaaggcgcc gacgaactca cctgcgacct ggagcaccat ctgggtgaac cctccgccga
    13321 ccgcaggatc gaacaacgct ctggacgacg cccacttggc gatggtcacg gccgggttga
    13381 tgtcaccctg gccccccatg gcaacgctcg tccagacgcc gaacagcacc gccatccccc
    13441 atccgaaggt gatgacgatc catcccgagt tctgcgcctt cgaacggttc aggagtacgc
    13501 ctgccacgac gccatcgcca agcaagatca ggacgccgat gccgatcagc atccgggacc
    13561 gatggccccg cacgaaggcc tcataccacc tccttcctcc ccactgactt cctccccact
    13621 gaccgacccg gggggatggt ctctgccgca aagcaaatgg cgttcaactg gcattgtcaa
    13681 atcgccacat cgctgtccgg ccatccgagc atcccgccgc tcgttcgaca tcgttgaaca
    13741 ttctcggggc aacccctcca ggtcctggag gggtcatcgt cccgcgcttg acaggcggtt
    13801 cctaccggcc gaacgttgcg ctctggcgag catatggacg ggtgggcgtg cgatcggatc
    13861 tgcggggtgg tggcggatgg aacctcagga cggcagggtg cgactcgatt ccggcttcat
    13921 cttcaggtgg ctggaggagc tcgccacgca ggtccgggag agccgcgact acctgaccca
    13981 gctcgacgct gcgatcggcg acgcggatca cggcatcaac atggaccgtg ggctgaccgc
    14041 cgcggtgcag aagatccgcg aactcggtga cccctgtccg ggagccatcc tgcaggcggt
    14101 ggggaccacg atgctgtacc tggtcggcgg ggcggccggt cccctctacg gggacgggtt
    14161 ccggaacatg ggtcgttccc tgggggagga cgccgagttc gatcccgagg gactgctccg
    14221 atgcctgcga gcggggctcg aggcgatcca ggcgatgggc gcggcctcgg agggggacaa
    14281 gacgatcgtc gatgccttcg cccccgggct gtgccggcct ggaacggggg ctgcgggccg
    14341 gggacccgct gatgacggcc ctcggccgag cgcgccgggc cgccgaggag ggcatgcggg
    14401 ccaccgtgcc gatgcaggcc cgcaagggca gggcgagcta cctggggccc cgcagcgtgg
    14461 ggcatcagga tcccggtgcc acgtccgcgg cgctgctgtt ctcggcgctg gagcgagccg
    14521 cggaccacga tgggtgtgga gggagcgctc ggccgtgatc cagtcggtgc tgcgggccgc
    14581 caggatcctg caggagctcg gggcgggctc acccaggctg ggcgtcaccg agctggctga
    14641 gcgcctgggc ttggccaagg caacggtcta cggcctgctg cgttctctcg aggccgagga
    14701 gttcgtggag cgtgacgggg aaaccggcaa gtaccggctg gggcccgcgc tgctccggct
    14761 ggggaacgcc ttcctcgaca accacgagct ccgtgctcgg tcgctgctgt gggcggagtc
    14821 gttggcggga cggctggtgg aggcggtccg ggtgggcgtg ctgaacgggg acagcgtcct
    14881 gatcgtccac cacgtcttcc gcccggacaa ctcggtccag atcctggagg tcggggcctc
    14941 gatcccctgg aacgcatgtg ccctgggcaa ggccatcgtg gcccacgtgg gccccaccag
    15001 gcgtgaggag ctcctggccg gcgagctgcc ccagctgacc gggcggacca tcgtggaccg
    15061 gggggagctg ggggccaccc tggatcgcgt cgcgagcgag gggttcgcca ccgaggatca
    15121 agaggggatc gtgggcgagg ctgaggtcgc ggccccggtg ttcgagcacc tggggaaccc
    15181 ggtgggcgcg atcggggtgg ccggtccggt cgagcggctc ctccaggacg ggccttcgaa
    15241 ggaggtcatc gcgaccgtgc gggaggtcgc gcggagcctc tcccgggaca tgggcgcggg
    15301 acggctcgcg cgggcccgct gagcccgccc accaggatgc ccgcacgccg gctcgggctg
    15361 ctggccctgg gcgtggtcgc ggtctccttc tccgcgatct tcatccgctg ggcggcggcg
    15421 cccgcgctgg ccgtggcgtt ctaccggaac gcgatggccg cggccgtgtt gttgccgatc
    15481 gctctggccc gccaccgcga ggagttccgc cggctctctc gccgggactg ggccatcgcg
    15541 ttgtcggcgg gcgggctgct cgccctgcac ttcgggctgt ggatcccatc gctctcgtac
    15601 accacggtgg ccgccagcac ggtcctggtg accagccagc cggtgtgggt ggccgtgatg
    15661 ggccggggga tgggggagtc ggcttcgggc gcaacgctgg gcggcatcgc gctctcgatg
    15721 ctgggggtcg cgatcatctc gggaggggac ctcgggctgt ccggccgggc cggtctcggg
    15781 gacctgctgg cgctcctggc ggccgtggcg gcggcgggtt acttcctctc gggccggacc
    15841 ctccgggccc ggctgtccct gctgacgtac gtggggatcg tctacacgac ctgctcggtg
    15901 ctgttggcgg cgacgatggc ggtggccggc acgccgttct cgggcttctc cctgcgcgtg
    15961 tgggggctgt tcgccctgat ggcgctggtc ccgcagttcc tggggcacac ggtcttcaac
    16021 tacctgctgg cgcacctgcg ggccacggtg gtggccgtcg cggtgaccgg cgagcccgtg
    16081 ggggccacgc tgctcgcgtt cgtgatcttg ggcgaggtcc cgaggtggac cgcgttcgcg
    16141 ggcggggcgg tcatcctggc ggggatctac gtgaccctga gcgcccagac ccgccggccc
    16201 gcgccggtgc ccatcgagtg accggagggg gtccccgttg gcccggatga cgacgccccg
    16261 cccgctgacg ccgtcgcacc ggtctgggac gatgaggccg catgagcggc atcgatctgc
    16321 actcgcacac cactcggtcc gacggcacgt tcacgccgac ggaggtggtc aacctggctg
    16381 ccgagctcgg gctcgagatg gtaggcgtca ccgaccacga caccaccgat ggtctcgggg
    16441 aggccgcggc ccgcggcctc gccgtcggcg ttcaggtcat ccccggggtc gagttctcca
    16501 ccgagtacca gggcacgagc gtgcacgtgc tctgctactg gatggatccg gccaacgagg
    16561 agttccgagc cgagctccgg cgcctgatcg acagccggtt ccgccggggc gagctcatgg
    16621 tcgagaagct ccgggcactc ggctacccga tctcgttcca gcgggtcagg cagatctcgg
    16681 gtgggggcaa catcgtccgg ccccacgtgg ctcgcgccat ggtcgaggcc gggatcgtga
    16741 aggacgagca ggaggccttc gaccggttca tcgccgatgg tggtcccgcc gccgtgccca
    16801 agcacgccct cgatcccgtg gacgcggtgg ggctgatccg gcgcgcgggt ggcgtgtgcg
    16861 tgctggccca tccgggcatg tggggtgacc agaccgaggt gccgacggag ctgatcgagg
    16921 ccatgaccgc cgtcggcatg gccggcctcg aggtggacca caccgatcac acgcccgagc
    16981 agcgggcgcg gttccgagcg ctggccgggc gactcggcct gatccccacc ggcggctccg
    17041 attgccacgg cacgcggtac aacccggtgc ggctcggcac cgtgaccacc gacccggagg
    17101 cgctcgcggc gctccgcggg ttggtgacgg ctagctgagc cctcgctcgc gttcgcgttc
    17161 gggcaggcgg atgcgtcgca gcaccggtcc gggttcccag ggcgtcccga ccgccggcag
    17221 cacccatcgg tccaacccga tgtacccggc gttgcgccag gccaggatca gcaggacctc
    17281 gaacacgaag aagaccgggt tcacccccgc cacgccggcc aggccgaacg acatcgtgag
    17341 cagccccgcc aggaaggcga agatccccgt gaccaccccg aggatcaggg cgatgccgaa
    17401 ggtgaactcc gcgatcgcga tgagcggagc gaggatcccg gccccatcgg aggagatcca
    17461 ccgcaggaag gccgcgtacc agccgtagtt cacggccgag tgcggcccct gcgccgcccc
    17521 ctgcagagcg ccggccgcga accccttcag cgaggcagag ctggtgatcc aggacgggct
    17581 cccgaagatc ttgtcccacc ccgagctggc ccattcgtag ccgaggtaca cgcggatgac
    17641 gagccagatc cacgccgccg caggcgacac gaacagccac ctcgcgacgc gcggttcctc
    17701 gaagtgcacg gtctcacgca tcaacggact ccacctcctc ggtgggcgtg cggatagccc
    17761 caacggtcgc gttcccgcgg cggggacggc cgaaacacgg cgggtcccgg tcgaaggcga
    17821 cctggcctcg tacgatgccc gcgtgggcgc actcgagatg gtcttcctgg acatcggggg
    17881 cgtcctgtac ggggacgcgg ggtaccggga ggcgctccgc cgggccctcc gagagctcgg
    17941 cgcgcggttc tcggaccagg agttcgacgc cgagtacgcg cggtgtcggc gggatcagtc
    18001 cgggtcgttc cggaggcgtc tgacccggcg gttcctggga cccgacgcgg acctggacgc
    18061 ggtggagcgg cacgcgggcg cctggtggca ctacacgccg gaggacctgg agccggatgc
    18121 gctccccacc gtccgcgagc tggcgcgcag ctatcggctg ggtctggtag cgaaccagcc
    18181 cagcggggta cgcagggcgc tggcgcgcga cggcctggaa ccgtacttcg aggtccgggc
    18241 ggtctccgac gacgttcgtc tggagaagcc ggacccccgg atcttcgtcc acgccctggc
    18301 ctcggcaggg gtccgcggtg accgggcggt gatggtgggg gaccggctcg actacgacgt
    18361 acgtccggcc cgggaggcgg ggatgcgcgc ggtctggctg ctccgaggcg aggcgccgga
    18421 cgatcccacc cccgagcagc tggcggagcc cgacgccgcc atccggtcgc tgcgggagct
    18481 gcgggaggtg ttggaggaac tgtgaacggc aagcgggtgt tggtggtggg tggcggctac
    18541 atcggcatgt acgccgccct gcggatggag cgtccgctgg acgaggacgg gcacgagctg
    18601 gtgctcgtga accccgagaa cttcatgacc tatcagccgt tcctgcccga ggtcgcctcc
    18661 ggcaacatcg agccccgcca cgtcctggtg ccgctgcggc aggtgctccg ccggacccgg
    18721 gtcatcgtgg gcgaggtcga gcaggtcgat cacccccgac gcacggcgac gatcaggacc
    18781 tcggacgggc aacggatgga actgccctac gacgtggtgg tgttgggagc cggctcgcgc
    18841 tcccgggtcc tgccggtccc cggcctggcc cggcacggga tcggcttcaa gacggtgcca
    18901 gaggcgatct acctgcgcaa ccggctgctg tcccgcctgg acgccgcggt cacggcgccc
    18961 gatgaagcat cccgcctccg ggccctcacc ttcgtgttcg tgggcgcggg gtacgcgggc
    19021 gtggaggcca tggccgagct ggaggacctg gcccgctccg ccgtgcgcta ctaccgggag
    19081 atctcccggg aggacatgcg ctgggtgctg gtggaggcgg ccgaccacat cctgccggag
    19141 atcgggacgg acctggccga acacgtccgt cgggagctcg agggccgcgg catcgaggtg
    19201 ctgctcggca cgcagttgaa gtcggccgag gccggggtga tccggctctc ggacggccag
    19261 tcgttccccg ccgacaccct ggtgtggacg gcgggggtgc ggccggagcc gctgggcgac
    19321 cgctcgggct tcccggtcga tgcctccggg cggatcgagg tggacgccta cctccgggtg
    19381 aagggcgtgt ccgatgcctg ggcggcgggg gacgcggccg cggtgcccga tctggtgttg
    19441 ggcgggatca cgccacccac ggcccagcac gccgtgcgac agggccgtcg gctgggggag
    19501 aacctggtgg cctcgctccg ggggcagccg ccgcggccgt tccggtggca caacctgggg
    19561 cagctcgcct cgctgggccg gtacaagggg gccgcgatgg tgctgggcgt gaggctgcgc
    19621 gggttcccgg cgtggtggct gcaccgcagc taccacctcg cgtacatgcc caccctgaac
    19681 cggaaggtcc ggatcgtggc ggactggacc gtggggctgc tattccctcg ggacgtggtc
    19741 cagctcggct ccctgcagcg gccccgcgaa gcgttccggc gagcggccgg ccgggagcgc
    19801 gactaggcca gcggctgcgc cgagcgcttg cgctggagct cgctggcggc cttcagcgcg
    19861 gcgtggctgc gctcgatgtc gggcagctcg tggccgccgg acagctcgtc ggcagcccat
    19921 cgggtgaggt cgcggatcga aacgatcccg accacctcct ccccgtcgac cacgggcaga
    19981 tggcggatgt tgcgggccac cataagctcg gcggcctcca ccacgtcggt ccggggcgtg
    20041 accgtctcca ggtggcgacg ggtcatgccc atcttcaccg tgacggtgtg gggatccgcc
    20101 ccggccgcca ccaggttcac gacgtcgcgc tcggtcagga tgccggccag gtgatggtcg
    20161 tccagcacca ccaccgagga gatgccgttc gcctgcatga tcttggccac gtcggcgaac
    20221 gcgtcctcgg ggttcgccgt cttcacgtcc gtgtgcatga tgtctccgac ctgcaccagc
    20281 gcacctccgt cctcggggaa gccgatgccc cagcgtaccg cgggccgtgt gggaggcgcg
    20341 agcgggccct cagtggtcgg agcgaccggt gaagttgaag ccgtcaacca ccaaggcggg
    20401 gacccgggag gccgagaaga agaactcgct ggccagctcg gtctcgcgcc cgatcgccac
    20461 cgtgtggctc agcgcctcca cgatcgattg cgtgaaccgg aggttcttca ccgggtgacg
    20521 gacctgtcca tcctccaccc agaacgtccc gtcccgggtc atccccgtga tcgaggactc
    20581 catggggttc accacgttcg agtagtggaa gcgggtcacc agcacgcccc ggtccacggc
    20641 tgcgatcatg tcctgcagcg aggccgggcc gggctcc
//
LOCUS       DASQQQ010000372         2806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_383079, whole genome shotgun sequence.
ACCESSION   DASQQQ010000372 DASQQQ010000000
VERSION     DASQQQ010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383079"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            429..1073
                     /locus_tag="VE646_14055"
     CDS             429..1073
                     /locus_tag="VE646_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009369057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZD81147.1"
                     /translation="MGADHVSSRDARAKKDAILDVAMRHFAEHGYRGTRVEDIAAEVG
                     VAKGTVFLHFTNKEGLFLAAYEKAVSMLPAWLDAPARVVQEGFWAVLDYWLERTEEFL
                     SEQWVPNRVAMIGRYDTGLGLRRPINRFMRSEDPYGTLEFVEFGVRRAEIRGDVDPEM
                     VASMLDWLAERFQDALSSEELDPGLVHRRPFDPERRERRIREFLLLLREGITER"
     gene            1078..2007
                     /locus_tag="VE646_14060"
     CDS             1078..2007
                     /locus_tag="VE646_14060"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HZD81148.1"
                     /translation="MNGPLLRAPVLWLTQNRAFRALATRTPIGRAVASRFVAGEDLDA
                     AVRVCRELEGRGIGAVLDHLGENVTAPAQAADAAAAYVAALERMRREPGLHAYISVKL
                     TQLGLDFSSELCIEYMEGVLAAAREADRLVMIDMESSVYVDRTLDVFGKLRSRHDRVG
                     LCLQTYLRRTPRDVDELPGGSVIRLVKGAYLEPPEVVLGSRREVAAAFARLAATLLER
                     GHTVHVATHDPALIAGAERFVERRGLASDRVEYQMLYGVRRDLQARLASNGRPVRVYV
                     PYGSEWYPYLTRRLAERPANMWFFLSNLVRMRR"
     gene            2021..>2806
                     /gene="proC"
                     /locus_tag="VE646_14065"
     CDS             2021..>2806
                     /gene="proC"
                     /locus_tag="VE646_14065"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HZD81149.1"
                     /translation="MNGRTVAFLGGGRMGEALISGLIRSGGRSADEIMVTCRREERAG
                     ELAERYGVTATLSNPDAVDRAETLVLMVKPQDMETLLGQIAPAVRPERHLAISFAAGV
                     RTSFVEKHLPGELPVVRVMSNVAVLVDEAMSVVAGGSLAEDKHLAVAEELLGYVGRVI
                     RLKETHLDAITATSGSGPAYFFLLAEAMIEACILLGLSRDVATELIIQTMLGSAKMLR
                     DTGRHPVELREMVTSPGGTTIAAIRHLEEAGVRAAFLNAIDAAC"
BASE COUNT          414 a          901 c         1041 g          450 t
ORIGIN      
        1 cactctttct ggcgggcctc ctacctgcgc atatgacctg tgaccagcgg atccatcgag
       61 ttgacctggt gtgcggtggc cgatagattt cgcgtcgctc cgagcagttg gagcggctga
      121 gtcagggaac cgaagcgggg acccctggga aagccgtaag gcgaactggg cgcacttggt
      181 cgtacggtcg ggaaggccaa ctggctgtgc gggcggggag gctcgggacg ccccaaagcc
      241 caacgaaagg gtctaccgcc aggaaaccga gacagctgtg gagattgctc ggagcacctg
      301 agcccgaggg gctgacggtg gctggaggct tcggcctccg gcgagccgga ggtccctcgg
      361 gttcttcttc gcttccccgg ccacggtttg tgagctccgg cgccggggga gtacgctccc
      421 gcccgcccat gggtgccgat cacgtatcct cgcgcgacgc gcgggccaag aaggacgcca
      481 tcctcgacgt cgcgatgcgc cacttcgccg agcacggcta ccgggggacc cgggtcgagg
      541 acatcgcggc cgaggtggga gtggccaaag ggaccgtctt cctgcacttc acgaacaagg
      601 aggggctgtt cctcgccgcc tatgagaagg cggtctcgat gctccctgcc tggctggacg
      661 ctccggcccg cgtcgtgcag gagggcttct gggcagtcct cgactactgg ctcgagcgga
      721 cggaggagtt cctgtctgag cagtgggtcc ccaaccgggt ggccatgatc ggccgctacg
      781 ataccggcct cggcctgcgc cgtcccatca accgcttcat gcgcagcgag gatccgtacg
      841 gcaccctgga gttcgtggag ttcggcgtcc gacgagcgga gatccggggc gacgtcgatc
      901 cggagatggt ggcatcgatg ctggactggc tggccgaacg gttccaggac gccctgagct
      961 cggaggagct ggatcccggc ctggtccacc ggcggccgtt cgatccggag cgacgggagc
     1021 gccggatccg ggagttcctg ctcctgctcc gtgaggggat caccgagcgc tgatggcatg
     1081 aacggaccgc tgctgcgagc gcccgtgctc tggctcaccc agaaccgcgc cttccgagcg
     1141 ctggccaccc gcacccccat cggtcgcgcg gtggcgtcca ggttcgtggc cggggaggac
     1201 ctggatgcgg cggtccgggt ctgccgcgag ctcgaggggc gcgggatcgg agccgtcctg
     1261 gatcacctgg gcgagaacgt gaccgccccc gcacaggccg ccgatgccgc ggcggcctac
     1321 gtggcggccc tggaacggat gcgccgagag ccggggctgc acgcctacat ctccgtgaag
     1381 ctcacgcagc tcgggctgga tttctcctcc gagctgtgca tcgagtacat ggagggggtc
     1441 ctggcggccg cgcgggaagc ggaccggttg gtgatgatcg acatggagag ctcggtctac
     1501 gtggatcgca cgctcgacgt gttcgggaag ctccggtcgc gccatgatcg ggtggggctc
     1561 tgcctccaga cctacctgcg acgcacgccg cgggacgtcg acgagctgcc ggggggctcc
     1621 gtcatccggc tggtgaaggg tgcctacctc gagccgccgg aggtcgtcct gggctcccgc
     1681 cgggaagtgg ccgcggcgtt cgcccgactg gcggccacgc tgctagagcg gggccacacc
     1741 gtccacgtgg cgacccacga tcccgcgttg atcgccggcg ccgaacggtt cgtcgaacgc
     1801 cgggggctcg cgtccgatcg ggtggagtac cagatgctct acggggtgcg gcgggacctg
     1861 caggcgcgac tggcatccaa cggccggccg gtgcgcgtct acgtgcccta cgggagcgag
     1921 tggtacccgt acctcactcg acgcctggcc gagcggcctg cgaacatgtg gttcttcctg
     1981 tcgaacctgg tgaggatgcg gcgatgagac aaggcgatcg gtgaacggcc gcacggtggc
     2041 cttcctgggg ggaggtcgga tgggggaggc cctgatctcg ggactgatcc gctccggcgg
     2101 gcgcagcgcg gacgagatca tggtgacctg ccgtcgcgag gagcgggcgg gggagttggc
     2161 cgagcggtac ggcgtgaccg ccaccctctc caacccggat gccgtggatc gagcggagac
     2221 gctggtcctg atggtgaagc cccaggacat ggagaccctg ctcgggcaga ttgccccggc
     2281 ggtgaggccc gagcgccatc tggcgatcag cttcgccgcg ggggtccgca cctcgttcgt
     2341 ggagaagcac ctgccgggcg agcttccggt ggtccgtgtc atgtcgaacg tggcggtcct
     2401 ggtggacgag gccatgtctg tggtggccgg ggggtcgctg gccgaggaca agcacctggc
     2461 cgtggccgag gagctgctgg gctacgtggg ccgggtgatc cgcttgaagg agacgcacct
     2521 cgacgccatc acggcgacca gcggctcggg cccggcgtac ttcttcctgc tggccgaggc
     2581 catgatcgaa gcttgcatcc tgctgggact ctcgcgcgac gtggccaccg agctcatcat
     2641 ccagaccatg ctgggttcgg cgaagatgct gcgggacacc ggtcggcacc ccgtcgagct
     2701 gcgggagatg gtgacctcgc cgggcggcac gacgatcgcc gcgatccggc acctggagga
     2761 ggccggcgtt cgagccgctt tcctgaacgc gatcgatgcc gcctgc
//
LOCUS       DASQQQ010000373         8895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_241007, whole genome shotgun sequence.
ACCESSION   DASQQQ010000373 DASQQQ010000000
VERSION     DASQQQ010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8895
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241007"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1930)
                     /locus_tag="VE646_14070"
     CDS             complement(<1..1930)
                     /locus_tag="VE646_14070"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZD81150.1"
                     /translation="MARKRGSADARIAVDIGGTFTDLVLLMGGRVTANVKVLTTPSDP
                     SVAVETGISRLLEIVDGDDVREVVHGTTLVVNALIERKGAVTGLVATEGFKDTVAIRR
                     ELRYDLYDLFLEMPQPLVPRRLRHELGERVLADGTEERPLDPEQVRRLARRLDREGVE
                     AVAVALLHSYRNPEHEREVAKLLRSELPGLYVSLSSDVSPELGEFTRTSTAIANAYVL
                     PLIDRYLEMLEKRLAGLGLTASLRIMLSTGGGGIAGVETARRFPVRLLESGPAAGAVS
                     AGFWGSRGGREDVLAFDMGGTTAKACLIERGIPLVAREFEAARVYRFTKGSGMPIRVP
                     VIDLIEIGAGGGSIAHVGPFGLPNVGPESTGADPGPACYGQGGDLPTVTDADLLLGYL
                     NPDFFLGGDMRLDVDGARSAMQRLGDGLGLSVEETAAAVHRVVNENMASAARMHAIER
                     GRDVRRCSFVATGGAGPVHAWGVARNLGIRKLVFPPSAGVASAFGMLTAAPGFELARS
                     LPSALSQVRWSEVRRAIRELERSGTRQLTDTGIPKDQVRVEIAADVRHRGQGDPITVE
                     LGSSLDDDPAGQVERAFEETYVRLYGRRPPGVESEVMTWRVRVAGPLPKLDVKLRTKA
                     GGKARKGTRRVWFAETDGY"
     gene            2035..4743
                     /locus_tag="VE646_14075"
     CDS             2035..4743
                     /locus_tag="VE646_14075"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HZD81151.1"
                     /translation="MQPDAGPARGRRSERAQIPAPLRRDVRILGRLLGRVLVESGGPE
                     LLQDVEALRQATITLREEPTQERRRGVTSLVDGFAVDRAALVARAFTVYFHLVNLAEE
                     RHRVRTLRERGRRGPVADSLEVAAEQIRGGDGPEVLGDVLGRLRITPVLTAHPTEARR
                     RSVVEALRRIAGLIDRLDAPDLPGSEAAAVERRMLEEITTLWRTDHVRRRRPDPLDEV
                     RSAMAVFDETIFRVVPLVYRELDRALDPTDPGGRPTRVPAFLRWGSWVGGDRDGNPTV
                     TADVTRTTLAIQSEHVLRGLETGARRIARSLTLSGRTSPASGELLASLKRDEGDFPAL
                     ASQLFLRFEDQPHRRKLLFAAARLVAARTRQRTGYPSPDHLIEDLELVQRSLLRAGAD
                     RLAFGEVQHLVWQAQTFGFHLASLEVRQHADVHAAALAELAPTAVGDASALDRLAQEG
                     WPWTAPARSPQAAEVLATLAAVADLQGRFGTEACHRYVVSFTRTAADVAAVRALARLA
                     APEGLPVDVVPLFESRVHLDAAPRVLDELLALPGVAAWLDERGRALEVMLGYSDSAKE
                     VGFLAANVALHRAQGGLAAWAARRGVELTLFHGRGGALGRGGGPTNRAILAQAAGSVA
                     GRFKVTEQGEVIFARYGDPDVARRHLEQVTSAVLLASTPSGASAARQGEERFGPLIAR
                     AGEASERAWRALVEGQGFADFFHRVTPVDEIRALQVGSRPAHRTGGGDLGSLRAIPWV
                     FAWTQNRVNLPGWYGLGSGLASAATEPEGLERLREMHRRWPFFRSVLENAELSLVKAD
                     LPIARRYLELGERPDLARLIEQEYERTVELVLSVSERDELLAERPLLRRAVELRNPYV
                     DALSFIQVRYLRQLREGSVSAEEAAQIARHILVTVNGVAAGLQNTG"
     gene            4789..6342
                     /locus_tag="VE646_14080"
     CDS             4789..6342
                     /locus_tag="VE646_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZD81152.1"
                     /translation="MTRTVTSLIAGKPAEGTGGNLRIPNPARLSEAVSEARLGDAATF
                     VKACRVAREAQVGWSAVPAPVRGRAIQQLGRLIEDNQGALARLLTREIGKPLVESLGE
                     IQEAIDTCSFFLSEGRRLYGQTVPSELPDKQLFTFRTPVGVAAIVTAGNFPVAVPSWY
                     LVPALLCGNAVVWKPAEYTPATSEAFAQLFLHAGIPEGVFNLVLADGPTTFGGLEQAL
                     EGGTVDKVGFTGSTAVGRRIGELCGRHLQSPCLELGGKNPLVVMPDANLDLAVEGALF
                     SGFGTAGQRCTSLGTVIVHRSVHEEFLARFATAVEMAPIGDPFQDVLFGPMISARFCD
                     RFLGWLELIRPHHGLHGSTATGRITGDAPRAGFVGDPEEGLYCHPTIVDGVTVDDELY
                     RTETFGPIVGVATFETFEEAMALANGHGYGLSSSIYTRDPLNVFRFRERISAGMVSVN
                     NSTSGAEAHLPFGGNGRSGNGSRQSGIWVLDQFTRWQAVNWDYSGRLQRAQMDVADVT
                     ADPSYRLEP"
     gene            6339..7598
                     /locus_tag="VE646_14085"
     CDS             6339..7598
                     /locus_tag="VE646_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZD81153.1"
                     /translation="MTEILTTVVRAGRLFDPVAGEVRRAQAIRIRGDRIEAVGPDRGT
                     DAAGASVVDLSAFTVTPGLIDCHAHLVGLEEDEGSVDFSALLGRTGAQEALAGVRNAH
                     RTLMAGFTTVRDVGTFRAFVDVALRQAIEDGWTPGPRMRCAGAYVTCSTGGGDIPPVA
                     PDVVVPRELRFGVADSVGEVRRVVREILHGGADFIKVIATGAVMTRGTNPGAPEFREG
                     EIRAAVEEAALYGTHVAAHAHGAEGIKRAVRAGVGSIEHGSLMDDEAIELMAERGTFL
                     VPDIYNGDWIERTGRAHGWPEEVLRKNEETTRAQREGFRKAVKAGVRIAYGTDSGIYP
                     HGLEARQLPYMVRHGMSPLQALRSATIEAAELLGWADRVGSISAGRYADLIAVTGDVL
                     DGELEDFADVPFVMKGGAVAKDMRPTP"
     gene            7680..8165
                     /locus_tag="VE646_14090"
     CDS             7680..8165
                     /locus_tag="VE646_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81154.1"
                     /translation="MKTCPYCAEEIKDEAIKCRWCLSWLVEEVPASAVTAPEGIAETK
                     PEEEEERPRSAAAPSASEPAPGLESVGGEPPAEPEIGSAEETQLMPQVSGAQDQVEFT
                     HSGERYILGYGREFFGIWDRQSPASPIQRFPRTDDGWRDAWNRYVSMEHNFMDLRTGS
                     G"
     gene            complement(8671..8741)
                     /locus_tag="VE646_14095"
     tRNA            complement(8671..8741)
                     /locus_tag="VE646_14095"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8707..8709),aa:Gly,seq:tcc)
BASE COUNT         1225 a         3097 c         3295 g         1278 t
ORIGIN      
        1 tgtaaccgtc cgtttcggcg aaccagacgc gtcgtgtccc cttgcgggcc ttgccgccgg
       61 ccttcgtgcg gagcttgacg tcgagcttcg ggagcgggcc ggccacgcgc acccgccagg
      121 tcatgacctc cgactcgacg cccggcgggc ggcgaccgta gagccgcacg tacgtctcct
      181 cgaacgcccg ctccacctgg cccgccgggt cgtcgtcgag cgagctcccc agctccaccg
      241 tgatggggtc gccctggccc cggtgacgga cgtcggccgc gatctccacg cgcacctggt
      301 ccttggggat gcccgtgtcg gtgagctggc gggtgccgct gcgctcgagc tcgcggatcg
      361 cccggcgcac ctcggaccag cggacctggg acagggcgga gggcaggctc ctggccagct
      421 cgaaccccgg cgcggcggtg agcatcccga aggccgaggc cacgccggcc gagggcggga
      481 agaccagctt ccggatgccg aggttgcggg ccacgcccca ggcgtgcacc gggccggcgc
      541 ccccggtggc gacgaacgag cagcgccgga cgtcccggcc gcgctcgatc gcatgcatcc
      601 gggcggcgct ggccatgttc tcgttcacca cccggtggac ggcggcggcg gtctcctcga
      661 cggacaggcc taggccgtcc cccaggcgct gcatcgcgga ccgcgccccg tcgacgtcca
      721 ggcgcatgtc gccgcccagg aagaagtcgg ggttcaggta gcccagcagc agatcggcgt
      781 cggtgaccgt gggcaggtcc ccgccctgtc cgtagcaggc cggaccgggg tcggcccccg
      841 tgctctcggg gccgacgttc ggcaggccga agggaccgac gtgggcgatg ctgccgcccc
      901 cggcgccgat ctcgatcagg tcgatcaccg ggacccggat cggcatgccg ctgcccttgg
      961 tgaaccggta gacgcgggcg gcctcgaact cgcgggccac gagcgggatc cctcgctcga
     1021 tcaggcaggc cttggcggtg gtgccgccca tgtcgaaggc caacacgtcc tcgcggccgc
     1081 cccgcgagcc ccagaacccg gcgctcaccg cgcccgcggc ggggccggac tcgagcagcc
     1141 ggaccgggaa ccggcgggcc gtctccaccc ccgcgatccc gccaccgccg gtggagagca
     1201 tgatccgcag cgacgccgtg agccccaggc ccgccagccg cttctcgagc atctcgaggt
     1261 accggtcgat cagcggcagc acgtaggcgt tggcgatggc cgtggaggtc cgggtgaact
     1321 cgccgagctc gggggagacg tcgctggaca gcgagacgta caggccgggg agttcgctcc
     1381 ggagcagctt ggccacctcc cgctcgtgct cggggttgcg gtacgagtgc aggagcgcca
     1441 ccgccaccgc ctccacgccc tcgcgatcca gacgccgggc cagccggcgc acctgctccg
     1501 ggtccagcgg ccgctcctcg gtcccgtccg ccaggacccg ttcacccagc tcgtggcgca
     1561 ggcggcgggg caccagcggc tggggcatct ccaggaacag gtcgtacagg tcgtagcgga
     1621 gctcgcggcg gatcgcgacc gtgtccttga agccctcggt ggcgaccagg ccggtcacgg
     1681 cacccttgcg ctcgatcagc gcgttgacga cgagcgtggt tccgtggacg acctcgcgca
     1741 cgtcgtcccc gtcgacgatc tcgagcagcc gggagatccc cgtctccacc gccaccgatg
     1801 ggtcgctcgg cgtggtcagg accttgacat tggccgtcac ccggccaccc atcaacagca
     1861 ccaggtcggt gaacgttcca ccgatgtcga cggcgatccg ggcatcggcg gagccccgtt
     1921 tcctcgccat acctcccccg ccggatcgat cggggacggc tgcgcttcgc gcgacgcgcc
     1981 ccgggtctct tgacgtcgag acatcataga ccatggtccc ggtaggatgc agccgtgcaa
     2041 ccggacgccg gcccggcccg cggccgccgg tccgagcgtg cgcagatccc agccccgctc
     2101 cggcgggacg tgcgcatcct gggccggctg ctggggcgcg tcctggtgga gtcgggcgga
     2161 ccggagctgc tccaggacgt ggaagccctc cgacaggcca cgatcactct gcgcgaggaa
     2221 cccacccagg aacgccggcg cggggtcacc tccctggtag acgggttcgc cgtcgatcgg
     2281 gcggccctgg tggcccgcgc cttcaccgtc tacttccacc tggtcaacct ggccgaggag
     2341 cggcaccggg tccggaccct ccgagagcgg ggtcggcgtg gacccgtggc ggactcgctg
     2401 gaggtcgcgg ccgagcagat ccggggtggg gacgggccgg aggtcctcgg ggatgtcctc
     2461 ggccgtctgc ggatcacccc ggtgctgacg gcccacccca cggaggcccg tcggcgctcc
     2521 gtcgtggagg ccctccgccg catcgccggc ctcatcgacc gactcgacgc tccggatctc
     2581 cccggctcgg aggcggcggc cgtggagcgc cggatgctgg aggagatcac gaccctgtgg
     2641 aggaccgatc acgtccgccg acgccgtccg gaccccctcg acgaggtgcg gagcgcgatg
     2701 gccgtgttcg acgagacgat cttccgggtc gtcccgctcg tctaccggga gctggaccga
     2761 gcgctcgacc cgaccgaccc gggcggccgc cccaccaggg tcccggcgtt ccttcgatgg
     2821 ggcagctggg tcggcggcga ccgcgacggc aaccccaccg tcacggcgga cgtgacccgg
     2881 accacgctgg cgatccagtc cgagcacgtc ctgcgggggc tcgagaccgg ggcgcggcgg
     2941 atcgcccgct cgctcaccct gtcgggccgg accagccccg ccagcgggga gctcctcgcg
     3001 tccctgaagc gggacgaggg cgacttcccg gccctggcct cccagctgtt cctgcgcttc
     3061 gaggaccagc cccatcggcg caagctcctg ttcgccgcgg cccgactggt agcggccagg
     3121 acccgccagc ggaccggcta tccctcgccc gatcacctga tcgaggacct ggagttggtg
     3181 cagcgctcgc tgctccgggc cggcgccgac cgcctggcgt tcggcgaggt gcagcatctg
     3241 gtgtggcagg cccagacctt cggcttccac ctggcctccc tcgaggtccg tcagcacgcg
     3301 gacgtccacg cggcggccct ggccgagctg gcgccgacgg cggtgggcga cgcctcggcc
     3361 ctggaccggc tggcccagga aggatggccc tggaccgctc cggcccgctc gccgcaggcc
     3421 gccgaggtcc tggccaccct ggccgccgtg gcggacctgc agggccggtt cgggaccgag
     3481 gcctgtcacc ggtacgtggt gagcttcacg cggaccgctg cggacgtcgc ggcggtccgg
     3541 gccctggctc gtctggccgc gcccgagggg ctccctgtcg acgtggtccc gctgttcgag
     3601 tcacgcgtgc acctcgatgc agctccccgc gtgctggacg agctgcttgc gctgcccggt
     3661 gtggccgcct ggctcgacga acgcggtcgg gccctggagg tgatgctcgg gtactccgac
     3721 tccgccaagg aggtggggtt cctggcggcg aacgtggcgc tccatcgggc ccagggcggg
     3781 ctcgccgcgt gggccgcccg ccgaggggtg gagctcacgt tgttccacgg acgtggggga
     3841 gcgctcggtc gaggcggtgg gccgacgaac cgcgccatcc tggcgcaggc cgccgggtcg
     3901 gtggcgggtc ggttcaaggt caccgagcaa ggagaggtga tcttcgctcg gtacggcgac
     3961 cccgacgtgg cccggcggca cctggagcag gtcacgagcg cggtgctgct ggcctccacg
     4021 ccctcggggg cgtccgcggc gcgccaggga gaggaacggt tcgggccgct gatcgcccgg
     4081 gccggcgagg cgtccgagcg cgcctggcgg gcgctggtgg agggccaggg cttcgctgac
     4141 ttctttcacc gcgtcacgcc cgtggacgag atccgggccc tccaggtggg ctcgcgtccg
     4201 gcccaccgga ccgggggcgg cgacctcggg tccctccggg cgatcccgtg ggtgttcgcc
     4261 tggacccaga accgggtgaa cctgccgggc tggtacggct tgggctccgg cttggcctcg
     4321 gcggcgaccg agccggaagg cctggagcgc ctgcgcgaga tgcaccggag gtggccgttc
     4381 ttccggtcgg tcctggagaa cgccgagctc agcctggtga aggcggacct gccgatcgct
     4441 cgtcgatacc tggagctggg cgagcgcccg gatctggccc gtctgatcga gcaggagtac
     4501 gaacgcaccg tggagctggt cctgtcggtg agcgagcgcg acgagctgct ggccgaacgc
     4561 ccgctgctcc gacgggcggt cgagctgcgg aacccgtacg tggacgccct ctcgttcatc
     4621 caggtccgct acttgcggca gctgcgcgag ggatcggtct cggcggagga ggcggcccag
     4681 atcgcccggc acatcctggt cacggtcaac ggcgtggccg cgggcctgca gaacacgggc
     4741 tgaggtggct accatcagcc gcatgatctc gagggcggga ggggcgcgat gacccgaacg
     4801 gtcacctcgc tgatcgccgg caagcccgcc gagggcaccg gcgggaacct ccggatcccg
     4861 aaccctgctc gcctgtcgga ggccgtgtcg gaggcccggc tgggtgatgc ggcgacgttc
     4921 gtgaaggcgt gtcgggtggc ccgcgaggcc caggtgggat ggtcggcggt ccccgcgccc
     4981 gtgcggggtc gagcgatcca gcagctgggg cgactgatcg aggacaacca gggggcgctg
     5041 gcccgtctgc tgacccggga gatcggcaag ccgctggtcg aatcgctggg ggagatccag
     5101 gaggccatcg acacctgctc gttcttcctc tccgagggcc ggcgcctgta cgggcagacc
     5161 gtgccgagcg agctccccga caagcagctc ttcacgttcc ggacgccggt cggggtcgcc
     5221 gccatcgtca cggccgggaa cttcccggtg gccgtgccct cctggtacct ggtgccggcg
     5281 ctgctctgcg gcaacgcggt ggtgtggaaa ccggccgagt acacgccggc cacgtcggag
     5341 gcgttcgccc agctgttcct gcacgccggg atcccggagg gcgtcttcaa cctggtgctc
     5401 gccgacggcc ccaccacgtt cggggggctg gagcaggccc tggaaggggg gaccgtggac
     5461 aaggtcgggt tcacgggctc cacggccgtc ggtcgccgta tcggcgagct ctgcgggcgg
     5521 cacctgcagt cgccctgcct ggagctgggg ggaaagaacc cgctcgtcgt gatgcccgac
     5581 gccaacctgg acctcgcggt ggagggggcg ctgttcagcg ggttcggcac ggccggtcag
     5641 cggtgcacgt cgctcggcac ggtgatcgtg caccggtcgg tccacgagga gttcttggcc
     5701 cggttcgcga ccgccgtgga gatggcgccc atcggcgatc cgttccagga tgtcctgttc
     5761 ggccccatga tcagcgcccg gttctgcgac cggttcctgg gctggctcga gctgatccgg
     5821 ccgcaccacg ggctgcacgg gtcgaccgcc accggccgga tcaccggtga cgcgccccgc
     5881 gctgggttcg tgggcgatcc ggaggagggt ctgtactgcc accccacgat cgtcgacggc
     5941 gtcacggtgg acgacgagct gtaccggacg gagacgttcg gcccgatcgt gggcgtggcc
     6001 acgttcgaga ccttcgagga ggccatggcg ctcgcgaacg gtcacgggta cggcctctcg
     6061 tcctcgatct acacccggga ccccctcaac gtgttccggt tccgggaacg gatctccgcc
     6121 ggcatggtga gtgtgaacaa ctccacctcg ggcgcggagg cgcacctgcc tttcggcggc
     6181 aacggccgga gcgggaacgg ctctcggcag tccgggatct gggtcctcga ccagttcacc
     6241 cggtggcagg cggtgaactg ggactactcg ggacggctgc agagggcgca gatggacgtg
     6301 gccgacgtga ccgccgaccc ctcataccgc ctggagccgt gaccgagatc ctcaccaccg
     6361 tggtccgggc cggccgcctg ttcgatccgg tagcgggcga ggtgcgtcgc gcccaggcga
     6421 tccggatccg aggggatcgg atcgaggccg tggggccgga tcgaggcacg gatgccgccg
     6481 gggcgtccgt cgtcgatctc tcggccttca cggtgacgcc gggcctgatc gactgccacg
     6541 cgcacctggt ggggctcgag gaagacgagg gatcggtcga cttcagcgcc ttgctcggac
     6601 gcacgggggc gcaggaggcg ctggccggcg tccgcaacgc gcaccgcacg ctgatggccg
     6661 gcttcaccac ggtccgcgac gtggggacct tccgcgcctt cgtcgacgtg gccctccggc
     6721 aggccatcga ggatggatgg accccgggcc ctcggatgcg gtgcgccggc gcgtacgtga
     6781 cctgctcgac cgggggtggg gacatcccgc ccgtcgcgcc cgatgtcgtg gtgccgaggg
     6841 agctccgctt cggcgtcgcg gactcggtgg gcgaggtccg gcgggtggtg cgggagatcc
     6901 tccacggcgg ggcggacttc atcaaggtga tcgccacggg ggccgtgatg acccgcggca
     6961 ccaaccccgg ggcgcccgag ttcagggagg gcgagatccg cgcggcggtg gaggaagcgg
     7021 ccctgtacgg gacccacgtg gccgctcacg cccacggcgc cgaaggcatc aagcgggccg
     7081 tccgagccgg ggtaggttcg atcgagcacg gctcgttgat ggacgacgag gcgatcgagc
     7141 tgatggccga gcgcgggacc ttcctggtgc ccgacatcta caacggggat tggatcgagc
     7201 ggaccggccg agcgcacggc tggcccgagg aggtcctccg caagaacgag gagaccacca
     7261 gggcccagcg ggaggggttc cgcaaggcgg tgaaggccgg ggtgcggatc gcctacggga
     7321 ccgacagcgg gatctacccc cacgggctcg aggcccggca gcttccgtac atggtccggc
     7381 acggcatgtc gcccctccag gcgctgcgga gcgccacgat cgaggccgcg gagctgctgg
     7441 ggtgggccga tcgcgtgggg tcgatctcgg ccggccggta cgcggacctg atcgcggtga
     7501 ccggagacgt cctcgacggc gagctcgagg acttcgcgga cgtgccgttc gtgatgaagg
     7561 gtggcgccgt cgcgaaggac atgcgtccca cgccgtgaac gaacgtcctc cgcggatcga
     7621 cgagggcccg gatgggggat gatgacctgg agagagcggg tgcccggaag gagcgtgcga
     7681 tgaagacctg tccctactgc gccgaggaga tcaaggacga ggctatcaag tgccggtggt
     7741 gcttgagctg gctggtcgag gaggttcccg cctccgccgt caccgccccc gaaggcatcg
     7801 cggagacgaa gccggaggaa gaggaggagc gacccaggtc ggccgccgcg ccctcagcct
     7861 cggagccggc tccggggctt gagtccgtgg gcggggagcc gccggccgag ccggagatcg
     7921 gttcggccga ggagacccag ctcatgccgc aggtgtccgg ggcgcaggac caggtggagt
     7981 tcacgcactc gggggagcgg tacatcctcg gctacggtcg agagttcttc ggcatctggg
     8041 accgccagtc gcccgcctcg ccgatccagc ggttcccgcg gacggacgat ggctggcgcg
     8101 atgcctggaa ccggtacgtg agcatggagc acaacttcat ggacctccgt acgggcagcg
     8161 gctgatcgtc cgttccggag agcgcccgaa ccgcagatct tgtgccgatc atgcagacgg
     8221 aagactcgat actcgacccg gcccgtggct cgaccgagcg ctcgaagccg gcgacaagga
     8281 ccggatcgcg ggctccttcg ccgaggaccg cgagctcgag ctgttgggcc tgcgactgac
     8341 cggtcgcgac ggcgtgagga cctggttgga ctgggtcttc acccatgccg cctcgttgcg
     8401 gttcaggcct cggctgatca cggtggaggg cgccaccttc gtcgaggagt tcgaagtgga
     8461 ggtaacgttg cgtgatggtc gtcggttgcg ttcacggtgg gcggagatcc tcgagttccg
     8521 cgagaacttc gtgaccagcc tctgccccta cttcgatccg ctcgagctcc tcgaggccga
     8581 agggcggatc gccgcggctc tcgctccgct ggtcaggagg ttcgccaggc ggcgtctgca
     8641 ccccctgatc ccaccctccg ttgagcttcg agcgggcgac gggaatcgaa cccgcaccgc
     8701 cagcttggaa ggctggggct ctaccattga gctacgcccg cggcagctcg aaagcctaac
     8761 agggtgctga aaaggccctc gtaggtccac gacttcgacg tccgcgtccg gcatccttca
     8821 cggaaccgaa gagagcgtcg gaggtgtcga tgcgcggaga gcccgagcgg cagatggcca
     8881 tgttgaccac gctca
//
LOCUS       DASQQQ010000374         7032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_241278, whole genome shotgun sequence.
ACCESSION   DASQQQ010000374 DASQQQ010000000
VERSION     DASQQQ010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241278"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1492)
                     /locus_tag="VE646_14100"
     CDS             complement(71..1492)
                     /locus_tag="VE646_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017973285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HZD81155.1"
                     /translation="MSERLGSGIERLDLVLDGGIPASSINLLVGPPGSGKTILAQHYL
                     FANATPERPAYYLSTVSEPFDKIVRYGQELSFFDIGSVGRSVFYEDLALILDRDGLAG
                     VSARIARLLQEADPSVIVIDSFKALHAFTNEPEAFRSFLHELAGRLSARSVTSFWVGE
                     YTREEIPSVPEAAVADAIVLLSFERSGARERRSLQVLKLRGSSYLSGTHAYRLSSSGM
                     EVFPRLADPVDQARYLQVPERSSSGIHALDVMLSDGYWPGASTLIAGPTGAGKTLMGL
                     HFVMGGARLGEPGIIATLQENPSQLERVARGFGWSLSEDNVHVMYRSPVDMYIDEWVY
                     ELLDLIQEKGARRVLVDSMSDLRISVDDELRFREYVYSLSQRMTRQNVSLMMTLELPE
                     VFSIGSISQMGVSHLSDNAILLQYTRSQSQVRRTVTVLKTRASYHDPLVREFLITPQG
                     IAVGEPIPGPGPAVGIPSALVTG"
     gene            complement(1489..2151)
                     /locus_tag="VE646_14105"
     CDS             complement(1489..2151)
                     /locus_tag="VE646_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZD81156.1"
                     /translation="MSEVRSPIYSIGAVSRMLGVPVSTLRTWEERYGTVIPDRSPGGQ
                     RLYSRLQIESLRRVCGWIEQGMSPSEAHRLLAERTQGQRNEPFPAPESLTLRILLAER
                     DPYAADMAEYLLKTEGFEVVLALDADDARRAVRDHPADVAIVELLISGGAGFDLCREL
                     RGGGVSRIVAVSQLDARDEALAAGADAFLRKPLEPLTLLATVKDVIGTSAFLRSPGGA
                     RV"
     gene            2300..2716
                     /locus_tag="VE646_14110"
     CDS             2300..2716
                     /locus_tag="VE646_14110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZD81157.1"
                     /translation="MDSDRGARIEGAAAQAASVKGPVPKVPRSAFFSIATERVGAHPC
                     VRLHGELDMLHEGDIRDALLELEKDGPAKLIVDLSGLSFIDSTGLRVLLEADARAREG
                     GWELVLAKGAQELPKVFTITMLDKRLTFVDDPSEVD"
     gene            2745..3161
                     /locus_tag="VE646_14115"
     CDS             2745..3161
                     /locus_tag="VE646_14115"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZD81158.1"
                     /translation="MLRVVPLPSGVASVPVARAEARDFAHGICPDRALDVETIASELV
                     TNVIRHARVGPATPLAMILEHGDGGMRLSVRDRGPGFDAPPGKPGRNQVSGWGLYLVE
                     RLADRWGFERQPDGFVVWAEIGSPMGHDAEADDGHD"
     gene            3145..4926
                     /locus_tag="VE646_14120"
     CDS             3145..4926
                     /locus_tag="VE646_14120"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZD81159.1"
                     /translation="MTDTTELSTDAGSALEEVGTAGSGLLRHPLWFPASRARLAGGLV
                     VAVAGCAAAAPLASTLTLHRFPGIGFLLVVLIATLVGRLASGLVAAVLSGVLLDVVVI
                     PPTDAFSRPKGPELPGLLIFLGASVAVAWIVTSREAAKARAETLALENADLLELERVA
                     RDDAEGTRDRLGLVSRVSELLALSMDYPGTYTRLAELVVGRLASLCLIDVIDESGQIT
                     RQAAVHQDPAKHAIVSRLMDFPPSLDGTHPVARVLRGGTAEYSPEIPPDFLRDTTRDE
                     EHLRIVRELGFQSFMCLPLATRGRILGTLTLVSTDPVRRFGQDDLTTAAEIARRAAVR
                     IDNARLYQQRDHIARTLQEVLLPRSLPKIAGFEMSAGYWPAREGLEVGGDFYDAFRRP
                     DGSVGLVIGDVCGKGAEAAAVMGVARQAVRVAGMSESRPSAILEVVNRSLLLGDFADR
                     FVTACDVRLRPREDGGMMTACSAGHPFPLVVRVDGSVKAIGEPGNFLGVFEDVKLTDV
                     AESIEPGDLLVLYTDGLVEWPHAPDAEGRFTALLSDHAGRSAPEVVAAAGEWWRTGVG
                     SAARDDAALLVVRSLPGSPAPPGAAAR"
     gene            5007..6266
                     /locus_tag="VE646_14125"
     CDS             5007..6266
                     /locus_tag="VE646_14125"
                     /inference="COORDINATES: protein motif:HMM:NF026235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HZD81160.1"
                     /translation="MRWSIAALIAVVIVGSIVVLAIRPPSPRGTRRSPSAGAEGRGRA
                     YRYIWTGTPGKRFSKAELDALARDDPLVVIEKGYGTTFQDWDAAARDLHARDPNAIVL
                     VDFLAGALPPALEARWGAAFDPAWILRDRNGDVMRDCSGGRCKYRVDVSDPAYRRFVE
                     GQVMKRLRAAPYGGVMYDNLHLYDRGRYPDLSDARIRELNAGFRSLLVETRRMIGPDH
                     LLFFNGVARSIGKEDVVDRGLGLLGAADGAQDEVFCYLDNQDAFRPVSELIQDDATYH
                     RLASQGATILESVKVENPAGQADAAHIERYCFGHYLMSYVPGHLRIQFKAYPTQGAGP
                     QIQENFTPEQTLDLGAPTADFTQDGQVLERRFENGWVFVNVSDSPQTIALPERLTLRT
                     GGTVGGTYRAGESYTIAPTDAAYFLRG"
     gene            6353..6793
                     /gene="rplM"
                     /locus_tag="VE646_14130"
                     /pseudo
     CDS             6353..6793
                     /gene="rplM"
                     /locus_tag="VE646_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719178.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
BASE COUNT         1038 a         2386 c         2562 g         1046 t
ORIGIN      
        1 gcaggggctc accttggagc gtgtgatcta cggacggcgg accgtgctct cggccaccga
       61 tctgtgaggc tcagccggtg acgagcgcgg acgggatccc caccgcgggc cccggccccg
      121 ggatcggctc gcccaccgcg atgccctggg gcgtgatgag gaactcccgc accagcgggt
      181 cgtggtagct ggcccgggtc ttcagcaccg tgacggtccg gcgcacctgt gactgcgacc
      241 gcgtgtactg gagcaggatc gcgttgtccg agagatggga gacccccatc tgggagatgc
      301 tgccgatcga gaagacctcg gggagctcca gggtcatcat caggctgacg ttctgccggg
      361 tcatccgctg gctcagcgag tagacgtact cgcggaaccg cagctcgtcg tccacgctga
      421 tccgcaggtc cgacatgctg tccacgagga cccgccgggc gcccttctcc tggatgaggt
      481 cgaggagctc gtagacccat tcgtcgatgt acatgtccac cggggagcgg tacatgacgt
      541 gcacgttgtc ctcggacagg gaccacccga atccccgggc cacgcgctcc agctgactgg
      601 ggttctcttg gagcgtggcg atgattccgg gctcgcccag ccgggccccg cccatcacga
      661 agtgcaggcc catcagggtc ttcccggcac cggtggggcc ggcgatcagg gtggaggcgc
      721 ccggccagta gccatcggac agcatgacgt ccagagcgtg gatgcccgag gagctccgct
      781 cggggacctg gaggtaccgg gcctgatcga cggggtccgc gagcctcggg aagacctcca
      841 tccccgagct cgacagccgg tacgcatgcg ttccggacag gtagctgctc cctcggagct
      901 tgagtacctg cagcgaccgg cgctctcggg caccgctgcg ctcgaacgag aggagcacga
      961 tggcatcggc cacggcggcc tcggggacgg aggggatctc ttcgcgcgtg tactcgccca
     1021 cccagaagga ggtgacggac cgtgcgctca gccgtcccgc gagctcgtgg aggaacgacc
     1081 ggaacgcctc cggctcgttg gtgaaggcat gcagggcctt gaagctgtcg atcacgatga
     1141 cgctcggatc ggcctcctgc agcaggcgcg cgatgcgtgc ggacaccccg gccaggccgt
     1201 cgcgatccag gatgagggcc agatcctcgt agaagacgga acggccgacg ctgccgatgt
     1261 cgaagaagct cagctcctgc ccgtaccgca cgatcttgtc gaacggctcg gagaccgtgg
     1321 acaggtagta ggcgggccgc tccggcgtgg cgttcgcgaa gaggtagtgc tgcgccagga
     1381 tcgtcttgcc cgaccccggg ggccccacca gcaggttgat ggatgacgcc gggatgccgc
     1441 cgtccaggac cagatcgagg cgctcgatgc cgctgcccag gcgctcgctc acactcgggc
     1501 gccgcccggg gaacgcagga aggcgctggt gccgatcacg tccttcaccg tggcgagcag
     1561 cgtcaggggc tcgagcggct tgcgcaggaa ggcgtcggcg cccgcggcca gggcctcgtc
     1621 gcgcgcgtcg agctgggaaa cggccacgat ccgcgagacc cctccgcccc ggagctcccg
     1681 gcagagatcg aaccccgccc ctcccgagat cagcagctcc acgatggcca cgtccgcggg
     1741 atggtcccgc accgcgcgcc tggcgtcgtc ggcgtccagg gcgaggacga cctcgaaccc
     1801 ctcggttttg agcaggtact cggccatgtc agccgcgtat ggatcccgct ccgccaacag
     1861 gatccggagg gtgaggctct ctggtgcggg gaagggctcg ttgcgttggc cctgggtccg
     1921 ctcggcgagc aggcggtggg cctcggacgg gctcatcccc tgctcgatcc agccgcacac
     1981 ccgtcgcagg gactcgatct gcagccgcga gtacaggcgc tggccgccgg ggctccgatc
     2041 ggggatcacc gtcccgtagc gctcctccca ggtccgcagg gtggagacgg ggaccccgag
     2101 catccgcgag acggctccga tgctgtagat gggacttctg acctcgctca tcgcactcag
     2161 gatacccccc tggatcgacc gatggaaagg ttatctcttc acgcgaagag gtttgttcgg
     2221 cgacacgatc cggccgagcg tgcactgagc gagtttcctc ttcacaaggt tcccacgatt
     2281 ggtgtagcat gctcacgtga tggactcgga tcgaggcgca cgtatcgagg gggccgcggc
     2341 ccaggccgca tccgtgaagg gaccggtccc gaaggtgccc aggtctgcgt tcttctcgat
     2401 cgccaccgag cgggtcgggg cccatccgtg cgtgcgtctg cacggcgagt tggacatgct
     2461 gcacgagggg gacatccgcg acgcgctcct ggagctcgag aaggatggcc cggcgaagct
     2521 gatcgtcgat ctgagcggcc tgtcgttcat cgactccacc gggctgcgag tgctgctgga
     2581 ggccgatgcc cgggcccgag agggcggatg ggagctggtc ctggccaaag gcgctcagga
     2641 gctgcccaag gtcttcacga tcaccatgct ggacaagcgt ctcaccttcg tcgacgaccc
     2701 ctcggaagtg gactaacgcc ccagatcgag gggatgatcg gtgggtgctc cgagtggtcc
     2761 cgctcccctc tggcgtggca tccgtgccgg tggcgcgtgc cgaggcccgg gacttcgctc
     2821 atggcatctg ccccgaccgt gccctggatg tggagaccat cgcgagcgag ctggtcacca
     2881 acgtgatccg gcacgcgagg gtcggtccgg cgacgcccct ggccatgatc ctggagcacg
     2941 gcgacggggg catgcgactg agcgtccgcg atcgcggtcc ggggttcgac gccccgcccg
     3001 gcaaacccgg ccggaaccag gtgtcgggat gggggctgta cctggtggag cggctcgccg
     3061 atcgctgggg gttcgagcgg cagccggacg ggttcgtggt gtgggcggag atcggatcgc
     3121 cgatgggtca cgatgcggag gccgatgacg gacacgactg agctctccac cgacgcgggg
     3181 tcggcgctgg aggaggtcgg cactgcaggc tcagggctgt tgcgccatcc tctgtggttc
     3241 ccggcgtcgc gcgcccggct ggccggcggg ctcgtggtcg ccgtggcggg gtgtgccgcg
     3301 gcggcgccgc tcgcatcgac ccttaccctc catcgcttcc cggggatcgg cttcctgctc
     3361 gtggtcctga tcgccaccct ggtggggcgg ctggcttcgg ggctggtggc ggcggtgctc
     3421 tcgggggtgt tgctggacgt cgtggtgatc cctcccacgg atgccttcag caggccgaag
     3481 gggccggagc tcccgggcct gctgatcttc ctcggggcgt cggtggccgt ggcgtggatc
     3541 gtcaccagcc gggaggcggc caaggcccga gccgagaccc tggccctcga aaacgccgac
     3601 ctgctcgagc tcgaacgcgt cgcgcgcgac gatgccgagg gcacccggga ccggctcggt
     3661 ctggtgtccc gggtcagcga gctcttggcc ctgtccatgg actacccggg aacctacacg
     3721 cgactggcgg agctggtggt gggacgcttg gccagcctgt gcctgatcga cgtgatcgac
     3781 gagtcgggcc agatcacgcg tcaggcggcg gtccaccagg accccgccaa gcacgcgatc
     3841 gtgagccggc tgatggactt ccctccgtcg ttggacggga cgcaccccgt ggcccgcgtg
     3901 ctgcgaggcg gcacggccga gtactcgccg gagataccgc ccgacttcct ccgggacacc
     3961 acccgcgacg aggagcatct gcgcatcgtg cgcgagctcg ggttccagtc gttcatgtgc
     4021 ctgccgctgg ccacccgggg ccggatcctg ggcacgttga ccctcgtctc caccgatccg
     4081 gtccgccggt tcggccagga cgacctgacc accgccgccg agatcgcccg ccgggccgcc
     4141 gtgcggatcg acaacgcgcg cctgtaccag cagcgggacc acatcgcccg gaccctccag
     4201 gaggtcctgc ttcccaggtc cctgccgaag atcgccggct tcgagatgag cgccgggtac
     4261 tggccggcgc gcgagggcct ggaggtcggc ggcgacttct acgacgcgtt ccgacggccg
     4321 gacggatcgg tcggtctggt gatcggcgac gtgtgcggca agggggccga ggcggccgcg
     4381 gtgatgggcg tggcccggca ggccgtccgg gtggcgggta tgagcgagag tcgtccgagc
     4441 gcgatcctgg aggtggtgaa ccggtccctg ctcctgggcg acttcgcgga tcggttcgtg
     4501 accgcctgcg acgtccgcct ccggcctcgc gaggacggcg ggatgatgac cgcatgcagc
     4561 gccgggcacc cgttccccct ggtggtgcgg gtggacggct cggtgaaggc gatcggggag
     4621 ccgggcaact tcctcggcgt gttcgaggac gtgaagctga ccgatgtggc cgagtccatc
     4681 gagccggggg acctgttggt cctgtacacc gacggcctgg tggagtggcc gcacgccccc
     4741 gacgcggagg ggcggttcac cgcgctgctc agcgaccatg ccgggcgctc cgcgcccgag
     4801 gtcgtggccg ccgccggcga gtggtggagg acgggcgtgg gttcggccgc ccgcgacgac
     4861 gccgccctgc tcgtggtccg gtcgctgccc ggtagccccg cgccgcccgg tgccgccgcc
     4921 cggtagcctc gagcggctat cgttcgtcgg cccgatcaca gcaggagggt cccatcgagc
     4981 gcggatcggc cggacgcagg cgcgccgtgc ggtggtcgat cgcggcgctg atcgccgtgg
     5041 tgatcgtggg atcgatcgtg gtgctggcca tccggccccc gagcccgcgt ggcacccgtc
     5101 ggtccccatc ggccggggcc gaggggcgcg gtcgcgcgta ccgctacatc tggaccggga
     5161 cccccggtaa gcggttctcc aaggccgagt tggacgccct ggcccgggac gacccgctgg
     5221 tggtgatcga gaagggctac ggcacgacct tccaggactg ggatgcggcc gcccgggacc
     5281 tgcacgcccg agacccgaac gcgatcgtgc tggtggactt cctggccggc gcgctgccgc
     5341 ccgcgctcga ggctcggtgg ggtgccgcgt tcgatccggc ctggatcctg cgggaccgga
     5401 acggtgacgt gatgcgggac tgctccggcg gccgatgcaa gtaccgggtg gacgtctccg
     5461 accctgccta ccggcgcttc gtggagggcc aggtgatgaa acgcctgcgg gcggccccct
     5521 acgggggcgt gatgtacgac aacctgcacc tgtacgaccg gggccggtac ccggacctca
     5581 gcgacgccag gatccgcgag ctgaacgccg gcttccgctc cctgctggtg gagacccgtc
     5641 ggatgatcgg cccggaccat ctgttgttct tcaacggtgt ggctcggagc atcggcaagg
     5701 aggacgtggt ggaccggggc ctcgggttgc tgggcgccgc cgacggcgca caggacgagg
     5761 tgttctgcta cctggacaac caggacgcct tccgccccgt ctcggagctg atccaggacg
     5821 acgccaccta ccaccggctg gcgtcccagg gggccacgat cctggagtcg gtcaaggtgg
     5881 agaacccggc cgggcaggcc gatgctgcgc acatcgagcg ctactgcttc ggccactacc
     5941 tgatgagcta cgtgccgggc cacctgcgga tccagttcaa ggcctacccc acgcaggggg
     6001 cgggtccgca gatccaagag aacttcaccc ccgagcagac cctcgacctg ggggccccca
     6061 cggcggactt cacgcaggac gggcaggtac tggagcggcg gttcgagaac gggtgggtgt
     6121 tcgtgaacgt gagcgactcg ccgcagacga tcgccctccc cgagcggttg acgctccgca
     6181 ccggcgggac cgtcggcggg acctaccggg cgggggagtc gtacacgatc gcccccacgg
     6241 acgccgccta cttcctccgg gggtgaggaa cgccaccgat ctgcgccttt gacgccgaag
     6301 cgaaaccgca ggtagcattg ctcgtcggcc gctccggccg acctcttcac gtatgaagac
     6361 ctacagtccg aggccgcgcg acatcgagcg ccgctggtac gtcatcgacg ccggcggtgc
     6421 ggtgctgggc cggctggcca cggaggtggc ctcgatcctg cggggcaagc acaagccgat
     6481 cttcgctcca cacatggaca ccggcgacca cgtgatcgtg gtcaacgcgg ccggggttcg
     6541 gctcaccggg ggcaaggagg acaagaaggt cgcgtaccgc cattcggggt atccgggcgg
     6601 gctcaccgcc accgggtacg ccaggctcct ggccgagcgc ccggcgttcg tggtggagaa
     6661 ggcggtgcgc gggatgttgc cgaagaatcc cctggggcgg tcgatgctga agaagctcca
     6721 ggtgtacgcg gggcccgagc atccccacca ggcgcagcag ccccggccgc tccggctggg
     6781 ggaggtcccc aggtgggagg gtctgcccgc gccgagggcc gccgccgagc cggagcccga
     6841 ggacgccaag cggcccgcgc cgaagcgctc caccgccaag cgggcggccc ggaccgctcc
     6901 cgcctcggcg accacgaccc agggcgaggc cccggccgag gctgaggccg aggcgccggc
     6961 ggcgaagcgc tccacgggga agcgctccac cgccaagaag accaccgcca agaagacggc
     7021 ggccaagaag ac
//
LOCUS       DASQQQ010000375        13144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_241641, whole genome shotgun sequence.
ACCESSION   DASQQQ010000375 DASQQQ010000000
VERSION     DASQQQ010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13144
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241641"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="VE646_14135"
     CDS             complement(<1..1284)
                     /locus_tag="VE646_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HZD81161.1"
                     /translation="MSAVEQWRGVAAEDAEELSGRVSALLQRRSRRLLGSLLAPHRGR
                     IWLLVLAVLVQNAALMAGPWLVGLGIDRGIPAVGQGHPLTLYVIVGAIVVAAAVQAVA
                     YRLFVMGAGRVGQDLLLDLRERVFGHFQRLSLAFHERYTSGRVISRLTSDLDAITELL
                     EAGFDTLVTSALSIVSIGVILVVLDRPLGLVALTALVPLTILSRWFQLESTTAYRRTR
                     AAVALVVTHFTESLAGIRAVHAFRREPRNDEIFARVNEDYRRATYRTFELVTIYWPGI
                     RLIGNVTTAAVLLYGGLRAVNGSLQVGVLAAFLLYLRRFFEPMAEISEFYNAFQGAAA
                     ALEKLSGVLEEDPSVPMPSAPAPEPRGGWLGEVRFQGVRFGYREGMEVLPHLDLTVPA
                     GQTVAMVGRTGAGKTTIARLLARFYDPDAGVVSVDG"
     gene            complement(1281..2975)
                     /locus_tag="VE646_14140"
     CDS             complement(1281..2975)
                     /locus_tag="VE646_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZD81162.1"
                     /translation="MLIMLGCAALGVGAGTIVPLIVMAIVNGPIRHGDRAQLIPMFLL
                     ALGLGVAEAGLIFGRRWVQQGVILRIERQIRDDLYGHLQRLPVSFHDRWQTGQLLSRA
                     TTDLSAIRRFFGFGAIFMVVNAVQVTTVIVVLIVLNAPLGILVGVTMVPVGWLGKRFG
                     TGYADVSRRVQDQQGDLATLVEESATGIRVTKAFGRGPLLQGRFGRAARTLRASSLEM
                     VRLRAGFWSLLELFPQLTMAAVVLVGVEGVGHGSLSLGALVAFTAMLVLLQWPVIDLG
                     WILALAQEAATAAERIYEVFDEPRAVADRPGAVALLEAKGRVAFEDVWFRYPSAGRDG
                     EDVLRGIDLVIEPGETLAVVGATGSGKTTLAGLVPRLYDVTRGRVTLDGHDVRELGLA
                     SLRRHVAVAFEDPTLFSMSVRENLTLGHPGATDAEIRSALELAQAEFVHDLPWGLDTR
                     IGDQGLSLSGGQRQRLALARAAITGPRVLVLDDPLSALDIHTEALVERALARVLRDAT
                     GLLVVHRPSTVALADRVALLQDGSLIAVGAHSELMVREPAYAAVLSATADEDLAAS"
     gene            3238..3951
                     /locus_tag="VE646_14145"
     CDS             3238..3951
                     /locus_tag="VE646_14145"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HZD81163.1"
                     /translation="MAAGRSEPFLPASPARTREPKFDMVRKGYDPTQVLEYLSRVADR
                     VQGLERRIQQLGSELEEARRQREAAMQAPPTEDLYESMSTRVADLMRTFDQDVERLRS
                     EAQAEAERIVADARSEAERVGQGIENLRQEARAQADRILAEARAESDRIRLDAQGKAE
                     DVRAAAERVEHEARERISRNVSDLENQRALLVEQLRDIRRRMLETLGSLEGIVQPAPE
                     DQGVVVVDAPDHEERSHSS"
     gene            4124..5011
                     /locus_tag="VE646_14150"
     CDS             4124..5011
                     /locus_tag="VE646_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81164.1"
                     /translation="MKRFAFVILVAGLICTVVPSASGAPAATVVHVTRLSTWHRPSPD
                     PTGIAYQRGTGRLIVVDSEVDETPWYKGANVWLKPLARRTPAGHWSTLRFSQEPTDVA
                     VRGPRTLVFTDDQRHRIFFVHPGSDHRWGTSDDVVSQIPTGAFGAQDAEGLAFAKQRK
                     RTFLLVLSGKRSMVFRLAPGRNGRFDGVPPTGDDVVRGRFSTAALGLTDPKGIAWDRR
                     SKLLYIVGHDQEMILRTRLRGALVDTIDLSATTIRHLSGITLAPGSRDGSVRHVYLTD
                     WGARHKNDGRIFEIAIHAG"
     gene            5140..5985
                     /locus_tag="VE646_14155"
     CDS             5140..5985
                     /locus_tag="VE646_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020512480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HZD81165.1"
                     /translation="MSGPTWLRRSPRLVLVVLVVGVALVAGAAAFATDEPAIHTLTPH
                     SEDQITNIDVLRQQIRNYYGDPFGTGRFSSDSYYAKEARSVAEEGREWIATASRTDHS
                     SATKAIVLDVDDTTLATWNYEVYSNWDYDPATNATFVTEQRFPKVPGMVRMVRKAVRK
                     GFAIFYLTGRPTSQEQATLGNLTNDGIGVDAGYPAPTTLPTGEDGLFTKPDVADYPDY
                     LQEACAADPGGKCTTVHYKSATRAYIESLGYEVVGNFGDQFSDLEGGHANRTFKLPNP
                     NYFLP"
     gene            complement(6154..6900)
                     /locus_tag="VE646_14160"
     CDS             complement(6154..6900)
                     /locus_tag="VE646_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81166.1"
                     /translation="MSDHLAALLLSGAAGFPTAVGALAGVRVRSSLAHSITFGAAAGA
                     TLGAVAFDLLPRAARLTSLWLVAAAAAAGLAVVYGYDLVMHAGVTAGPKAEQRERAET
                     LHRRHGRGRGLAVLGGSLMVMRLAHGAVLGLAAGGTGVALATAFGTSNLNDGFNLGQL
                     GLADRRSGAALGIWVSATIIALMGGAAASAWWLTGTAAWIRASVAAFGAGTLIYVSSA
                     HFISEAHAHEFARSSTLALFGAFIAIFWLS"
     gene            complement(7059..7283)
                     /locus_tag="VE646_14165"
     CDS             complement(7059..7283)
                     /locus_tag="VE646_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81167.1"
                     /translation="MATDTELAELCRAALDAESDWRYRGAEGRRAAYERFQQLVRTIA
                     EATGRSYEEAFNEAIEQWVKEHFCAVAGEG"
     gene            complement(7414..9098)
                     /locus_tag="VE646_14170"
                     /pseudo
     CDS             complement(7414..9098)
                     /locus_tag="VE646_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551684.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            complement(9171..10250)
                     /locus_tag="VE646_14175"
     CDS             complement(9171..10250)
                     /locus_tag="VE646_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HZD81168.1"
                     /translation="MRVVYSPEHLHHAPQHEVSDGRAIELYETPARAESIRAALEADG
                     GFELEEPSGHGHGPIEAVHDPEMIGFLEELWGDWVESGAGSPEIFPDTFPLPALREGM
                     GPGPAPATPLGRIGAWCFDTATPIVEGTYAAACAAVDVALTAADVVLGGERVAYGLCR
                     PPGHHAARRAFGGYCFFNNAAIVADHLAMRTTEPVAILDVDYHHGNGTQQIFYERGDV
                     LYVSLHGDPDRAYPYFTGFADERGVGEGEGTNVNLPLPAGCTDERYLQDLETAIAAIA
                     AFGGDTLVISLGIDTYGRDPICDLALTTPVYHEVGKRVAALDRRTIVLQEGGYFVPHL
                     GENVRQWLRGFEGRDLDLSRLVDVT"
     gene            complement(10277..10882)
                     /gene="rdgB"
                     /locus_tag="VE646_14180"
     CDS             complement(10277..10882)
                     /gene="rdgB"
                     /locus_tag="VE646_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018542264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HZD81169.1"
                     /translation="MSFPPRLAIASRNPGKIREIVAICADWPVEWDRARPGDGSWPEI
                     EESGNTYLDNALLKAHGVARARQTPALADDSGIEVDALGGAPGPRSARYAGPHATDQR
                     NLAMLVRALAGIPAAGRTARYRCVSAVAWPDGREVWAEGHCEGTLISKPRGSGGFGYD
                     PIFVPAGWDRTMAELDAAEKDRISHRGRALRALRDLFVREP"
     gene            complement(10879..11601)
                     /gene="rph"
                     /locus_tag="VE646_14185"
     CDS             complement(10879..11601)
                     /gene="rph"
                     /locus_tag="VE646_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HZD81170.1"
                     /translation="MSDRPDGRGPADLRPVKVELGYQEWAEGSVLLEMGRTKVLVAAS
                     FEDRAPRFLHGTGRGWVTAEYSMLPRATTARTPREVGRGRPSGRTQEIQRLVGRSLRS
                     VVDLAAMGEGTIWVDCDVLQADAGTRTASITAGYIALALAVRSLRDRGALKREVLTDS
                     VAAVSAGILDGRAVLDLSYEEDAGADVDFNVVMTGGGRLVEIQGTAEGSPFSRQDLSS
                     LLDLAEVGIRQLTEIQRQALGA"
     gene            complement(11598..12350)
                     /locus_tag="VE646_14190"
     CDS             complement(11598..12350)
                     /locus_tag="VE646_14190"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZD81171.1"
                     /translation="MELIVLGATGSWPDRGTALSGYLIRQDGFNLWVDAGSGTMANLQ
                     KHISIADVDAMIITHEHADHCVDVYPLFYARYYAGMGANGMPLYIPKGFGDRVVALLS
                     EESIEGFRIGFDVHEVSPEDGFEVGPFRVSASAMSHLGLPALGFRIESNGSALAYTGD
                     TGPTPAAVDLARDADLFLSEATYQDADELQPFHLSARQAGGYAKAGGVGRLVLTHLLP
                     THDRERSRSEAADVYDGPVDVAVEGSRFEVGE"
     gene            complement(12341..>13144)
                     /gene="murI"
                     /locus_tag="VE646_14195"
     CDS             complement(12341..>13144)
                     /gene="murI"
                     /locus_tag="VE646_14195"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HZD81172.1"
                     /translation="VFDSGVGGLTVARAVLDLLPNEPVLYVGDNARFPYGPRPLEQIR
                     SFALETAGYLVSRDVKMLVVACNSIEVAAIRQVTDAAAVPVVGVIDPGARAAVRTTRN
                     RRVGLIGTEATVASGAYDRAVGAAEPEVRLFSRACPLFVEHVEAGDTSSPELLAAAKE
                     YLEPLREEGVDTLVLGCTHYPMLSGLIQMVMGNEVVLVSSAEETAKDVYATLVERDLL
                     RPSGAPPVHEFLTTGDPERFRAVAHHFLGPEIGPVASVEARRIEEATWS"
BASE COUNT         2045 a         4849 c         4505 g         1745 t
ORIGIN      
        1 gccgtccacg ctcaccacgc ctgcatcggg gtcgtagaag cgggccagga gccgggcgat
       61 ggtggtcttg ccggcccccg tcctgccgac catcgccacg gtctgcccgg ccgggaccgt
      121 gaggtccaga tggggcagca cctccatccc ctcccggtag ccgaagcgca cgccttggaa
      181 ccgcacctcg cccagccaac cccccctcgg ctcgggcgcg ggggccgagg gcatggggac
      241 cgacggatcc tcctccagca cgcccgagag cttctccagc gcggcggcgg ccccctggaa
      301 cgcgttgtag aactcgctga tctcggccat cggctcgaag aaccggcgca ggtacaggag
      361 gaacgcggcc agcacgccca cctgcaggga gccgttcacc gcccggagcc ccccgtacag
      421 gagcacggct gcggtggtca cgttgccgat cagccggatc cccggccagt agatcgtgac
      481 gagctcgaac gtccggtacg tggcgcggcg gtagtcctcg ttcacacggg cgaagatctc
      541 gtcattgcgc ggctcccgtc ggaacgcgtg gaccgcgcgg atccccgcca gcgactcggt
      601 gaagtgggtc accaccaacg ccacggccgc tcgggtccgc cggtaggccg tggtggactc
      661 cagctggaac caacgggaga ggatcgtcag cgggaccagc gcggtcaggg ccaccaggcc
      721 cagcggccgg tccagcacca ccaggatcac accgatgctc acgatcgaca gggcggaggt
      781 gaccaacgtg tcgaacccgg cctccagcag ctcggtgatg gcatccaggt cggaggtgag
      841 ccgagagatc acccgtcccg aggtgtaccg ctcgtggaag gccaggctga ggcgctggaa
      901 gtgcccgaac acccgctccc gcaggtcgag cagcagatcc tggccgaccc ggcccgcccc
      961 catcacgaac agccgatagg cgaccgcctg cacggcggcc gccaccacga tcgctcccac
     1021 gatcacgtac agggtgagcg ggtggccctg ccccacggcc gggatgccgc ggtcgatgcc
     1081 gaggcccacc agccagggtc ccgccatcaa ggcagcgttc tggaccagca cggccagcac
     1141 caacaaccag atccggcccc ggtgcggcgc gaggagggac cccagcaggc gccggctcct
     1201 gcgctggagc agggccgaga cccgtcccga gagctcctcg gcgtcctcgg cggccactcc
     1261 tcgccactgc tcgacggcgc tcatgaggcg gccaggtcct cgtcggcggt ggcagacagg
     1321 accgcggcgt aggccggctc ccgcaccatc agctcgctgt gggcccccac cgcgatgagg
     1381 gacccgtcct gcagcagcgc cacccggtcg gccagggcca cggtggaagg ccggtgcacc
     1441 accagcagac cggtggcgtc gcggagcaca cgggccaagg cccgttcgac cagcgcctcc
     1501 gtgtggatgt cgagcgccga gagcgggtcg tccagcacca gcacccgcgg cccggtgatg
     1561 gccgcccggg ccagggccag ccgctgccgc tgcccgccgg agagggacag gccttgatca
     1621 ccgatccgcg tatccagccc ccacggcagg tcgtgaacga actcggcctg ggccagctcc
     1681 agcgcggacc ggatctcggc atcggtcgcc cccgggtggc cgagggtgag gttctcgcgc
     1741 acgctcatgg agaacagcgt ggggtcctcg aaggccacgg cgacgtgtcg ccgcagcgag
     1801 gccaggccga gctcgcgaac gtcgtggccg tcgagggtga cccggccgcg cgtgacgtcg
     1861 tacagccgag gcaccagccc ggccagggtg gtcttccccg aacccgtggc ccccaccacc
     1921 gcgagcgtct cccccggctc gatcaccagg tcgatcccgc gcaggacgtc ctcgccgtcc
     1981 cgtccggcgg acgggtatcg gaaccacacg tcctcgaagg cgacgcgccc cttcgcctcg
     2041 agcaaggcca ccgcgcccgg tcgatcggct accgctcgcg gctcatcgaa gacctcgtag
     2101 atccgctctg cggcggtggc ggcctcctgc gccagggcca ggatccaccc cagatcgatg
     2161 accggccact gcaggagcac gagcatggcg gtgaaggcca ccagcgctcc gagcgacagg
     2221 ctcccatggc ccacgccctc cacccccacc aggaccacgg ccgccatcgt gagctgcggg
     2281 aagagctcga gcagggacca gaagccggcc cgcagacgaa ccatctcgag cgacgacgcg
     2341 cggagggtcc gggccgcccg gccgaaccgc ccttgcagca gcggcccccg cccgaacgcc
     2401 ttggtcaccc tgatcccggt ggcggattcc tcgaccagcg tggccaggtc gccctgctga
     2461 tcctggaccc ggcgggacac gtccgcgtac ccggtcccga accgcttgcc gagccacccc
     2521 accggcacca tggtgacccc caccaggatg ccgagtggag cgttcaagac gatcagcacg
     2581 acgatcacgg tggtcacctg caccgcgttg accaccatga agatcgcccc gaacccgaag
     2641 aaccgccgga tcgcgctgag gtcggtggtg gcgcgcgaga ggagctggcc ggtctgccac
     2701 cggtcgtgga aggagaccgg caagcgctgg agatgaccgt agagatcgtc ccggatctgt
     2761 cgctcgatcc gcaggatcac cccttgctgg acccatcgtc ggccgaagat caaccccgcc
     2821 tcggccacgc ccaagcccag cgccagcagg aacatcggga tcagctgggc ccggtcgccg
     2881 tgacggatcg gcccgttcac gatggccatg acgatgagcg gaacaatcgt tccggcgccg
     2941 acgcccagag cggcgcagcc cagcatgatc agcatccggc cccggtaggg ccggatgtag
     3001 ctccgcatgc gccacagcgg tcggaaccgt cccaggaagg tcacccacag agaatctcac
     3061 ccgaggggac cttcaccggc gagccccacg gtcgaggttc cagcccgcgg ctctcgcatg
     3121 gggactcgcg cagatggacc agcggtcgtg atgaggatgc gggtggcgat acgcttcccg
     3181 cccgaggtag ggtcgttgga tcggaggaac ttcgttcgcc acccgagagg gggacgtatg
     3241 gccgccggaa gatcagagcc gttcctgccc gccagcccag cccgcacccg ggagcccaag
     3301 ttcgacatgg tgcggaaggg ctacgacccc acccaggtgc tcgagtacct gagccgggtc
     3361 gcggaccggg tccagggact ggaacggcgg atccagcagc tgggctcgga gctcgaggag
     3421 gcccggcggc aacgggaagc ggcgatgcag gccccgccca ccgaggacct gtacgagtcc
     3481 atgtcgacac gcgtggcgga tctgatgcga accttcgacc aggacgtcga acggctccgc
     3541 tcggaggccc aagcagaagc cgagcggatc gtggccgatg cccggagcga ggcggaacgc
     3601 gttggccagg ggatcgagaa cctccgtcag gaagcccggg cccaggccga ccggatcctc
     3661 gccgaggctc gggccgagtc cgaccgcatc cggctcgatg cccagggcaa ggccgaggac
     3721 gtacgcgcgg ccgccgagcg ggtcgagcac gaagcgcggg agcgcatctc ccgcaacgtg
     3781 tccgatctgg agaaccagcg ggcgctgctc gtcgaacagc tccgggacat ccgccggcgc
     3841 atgctcgaga cgctcgggag cctggaaggg atcgtgcagc cggcgccgga ggaccagggg
     3901 gtcgtggtgg tcgacgctcc cgaccacgag gagcgtagcc acagcagctg atccgggccg
     3961 ggtccggcgg ctcggggacc gccgcgaggg taccgcacgc tcggtacccc gggtacggcg
     4021 tccggtggat gcgtccaccc caacgcttcg cccatgatgc gcaggcagga accgcggctc
     4081 gtctgcgcgt ggccggccgg gccgcagccg ggaaggagtg gatgtgaaga ggttcgcgtt
     4141 cgtcattctc gtggcggggc tcatctgcac ggtcgtgccc tcggcctctg gtgcaccggc
     4201 ggccacggtg gtccacgtga cccggttgtc cacctggcac cgtcccagtc cggatcccac
     4261 cggcatcgcg tatcagcgtg gcaccggccg gctcatcgtg gtggacagcg aggtcgacga
     4321 gacgccctgg tacaagggtg ccaacgtctg gctgaagcct ctggcccgaa ggaccccggc
     4381 cggtcattgg tcgaccctgc gcttctccca ggagccgacg gacgtcgcgg tgcgtggccc
     4441 ccgcacgctg gtcttcaccg acgaccagcg ccaccggatc ttcttcgtgc acccgggatc
     4501 ggaccaccgc tgggggacca gcgatgacgt ggtgagccag atccccaccg gagcgttcgg
     4561 ggcccaggac gcggagggcc tcgcgttcgc caagcagcgc aagcgcacct tcctgctggt
     4621 gctgagcggt aagcggtcga tggtgttccg gctggccccg ggtcgcaacg gtcggttcga
     4681 tggcgtgccc ccgaccggtg acgacgtcgt acgcggacgc ttctccaccg cggccctggg
     4741 tctgaccgat ccgaagggga tcgcctggga taggcggtcg aagctgctgt acatcgtcgg
     4801 tcacgaccag gagatgatcc tccggaccag gctccggggc gccctggtcg acacgatcga
     4861 cctgtccgcg accacgatcc gacacctgtc gggcatcacc ctggcgccgg gcagcaggga
     4921 cggctcggtg cggcacgtct acctcacgga ctggggcgcc cgccacaaga acgacggccg
     4981 gatcttcgag atcgccatcc acgcgggatg acggcctgat ccgaccggtc gttccctggg
     5041 acccgagccg cggaccggtg cgcctgcggg gctcccatcg cctccgcgcg cgtgtatatg
     5101 ctcctgggaa ccggaagtct cacggaagga ggggctctcg tgagcggtcc cacgtggttg
     5161 cggcgctcgc cgcgtctggt cctcgtggtg ctggtggtag gggtggcgtt ggtcgccgga
     5221 gcggccgcgt tcgccaccga cgagccggcg atccataccc tcacgccgca ctcggaggac
     5281 cagatcacca acatcgacgt gctccgtcag cagatccgga actactacgg tgaccccttc
     5341 ggcaccggga ggttcagcag cgacagctac tacgccaagg aggcccgctc cgtcgccgag
     5401 gaaggccgcg agtggatcgc gaccgcttcg cgcaccgacc actcgtcggc gaccaaggcg
     5461 atcgtgctgg acgtcgacga caccacgctc gcgacctgga actacgaggt ctacagcaac
     5521 tgggactacg acccggccac gaacgccacg ttcgtgaccg agcagagatt ccccaaggtg
     5581 cccggcatgg tccgcatggt caggaaggcg gtccgcaagg ggttcgcgat cttctacctc
     5641 accggccgtc ccacctcaca ggagcaggcc acgctcggca acctgacgaa cgacggcatc
     5701 ggggtggacg ccggctaccc ggcaccgacc accctgccca ccggtgagga tgggctgttc
     5761 accaagcccg acgtcgccga ttaccccgac tacctgcagg aggcctgcgc ggcagacccg
     5821 ggcggcaagt gcacgacggt gcactacaag tcggccaccc gcgcctacat cgagtcgctc
     5881 ggctacgagg tcgtcgggaa cttcggcgat cagttcagcg acctcgaggg cggtcacgcc
     5941 aaccggacgt tcaagctgcc gaacccgaac tacttcctcc cgtaagccaa gagcacacac
     6001 ccggtccggc cccgcggccg gaccgggtgc tccggccggg aaccttgcct cgcaggctca
     6061 ctcggtatgg gccgggggtc gaccgtggag cgggaccggg ggcccggccg tggtcaggca
     6121 ggtgggtagg gggcggacag ccgcgcccgc gcctcaggag agccagaaga tcgcgatgaa
     6181 ggcgccgaac agcgccagcg tcgaggagcg cgcgaactcg tgggcgtgcg cctcggagat
     6241 gaagtgggcg gacgagacgt agatcagggt tccggccccg aacgcggcca ccgaggcgcg
     6301 gatccatgcg gcggtgcccg tgagccacca ggccgacgcc gcggcacctc ccatcagcgc
     6361 gatgatcgtg gcggacaccc agatcccgag cgccgcgccc gagcgtcgat cggccagacc
     6421 gagctggccg aggttgaagc cgtcgttcag gttcgaggta ccgaacgccg ttgccagcgc
     6481 gacccccgtg ccgcccgcag ccagccccag gaccgcaccg tgcgccagcc gcatcaccat
     6541 caggctccct ccgagcacgg ccagcccgcg cccccggcca tggcgacggt ggagggtttc
     6601 cgcgcgctcc cgttgctccg ccttcggccc tgcggtgacc cccgcgtgca tcaccaggtc
     6661 gtagccgtag acgacggcca ggccggcggc cgcggccgcc gcgaccaacc acagggaggt
     6721 gagccgtgcg gcccgaggga gcaggtcgaa cgccaccgct cccagggtgg ccccggccgc
     6781 cgccccgaag gtgatcgagt gggccaggct gctgcgcacg cggacccccg ccagcgctcc
     6841 cacggccgtg gggaaccccg ccgcgccgga caggaggaga gccgccaggt ggtcgctcac
     6901 cggagagggg tacccgtgcc gggtcctcga cacgcgggcg accccggtgg gggccgatca
     6961 gaccgggccc accggggtca ccgtgagatg cgtacccggg aacccgcccc tgcacgcagg
     7021 cctacacgtt cgcctggttg cccgagccgc tcccgggctc acccctcgcc ggccaccgcg
     7081 cagaagtgct ccttcaccca ctgctcgatc gcctcgttga acgcctcctc gtaggagcgg
     7141 cccgtggcct cggcgatggt gcggaccagc tgctggaagc gctcgtaggc ggcgcgacga
     7201 ccctccgccc cccggtaccg ccagtcgctc tccgcgtcca gcgccgcccg gcacagctcg
     7261 gcgagctccg tatccgttgc catcgcgacc tccggttccc tctgggacgc gcgaacctca
     7321 catcgagggt ccatgtccgt gcagaccgtc cccagggccc ttggtcccgt cccagaccgg
     7381 accttcgccc accgcggccg cccggccgac ggctcaacgc ctgttcgagc cggccgtcgg
     7441 cgccgatagg ccgagctcgt cgatggagat ctcgcgcatc cggtacttct ggatcttccc
     7501 ggtcacggtc atggggaact cgtcggtgaa gcggacgtac cgggggatct tgtacgaggc
     7561 gatgcgtccg cggcagaagg atcggatctc ctcctccctg gcccgggctc ccgacttccg
     7621 tcggacccag gcgcagacct cctcgccgta cgtggggtcg ggcacgccca ccacgtgcac
     7681 ctcggccacc gtggggtggg tgaagaggaa gtcctcgatc tcccgcggat acacgttctc
     7741 gccgccgcgg atgatcatgt ccttgatcct gcccacgatc tccacgtagc cgtcgtgccg
     7801 catgatcgcc aggtcgcccg agtgcatcca ccgggcccgg tcgatcgcct gctgggtggc
     7861 ctcggggtcc tcccaatagc ccagcatcac cgagtacccg cgcgtgcaca gctcgcccgg
     7921 cgcccccctg gggacggtgg ccccggtgac cgggtcgagg atcttggcct cgacgtgcgg
     7981 atggatcgcc ccaccgtcga gacacgacgg tccaggtcgt catgggggag cgactggaac
     8041 gagacgggcg aggtctcggt catgccgtag cagatcgtga cctcgtgcat gtgcatgcgg
     8101 tccacctcct gcttcatgac ctccacggga cagggggctc cggccatgat cccggttcgc
     8161 aggctgctca ggtcgaagcg gtcgaacccg gggctgccga gctccgacac gaacatcgtc
     8221 ggcaccccgt acagagccgt gcaccgttcc cgctcgaccg cctggagcac cgcgtgcggg
     8281 tggaacgcct cgccgggcag caccaccgtc gtgccgtggg tgagcgccgc cagggtgccc
     8341 atcaccatcc cgaagcagtg atagaagggc accggcacgc agatccggtc gcccggcacg
     8401 aggcccagcc gctcgccaac gaagaagccg ttgttgagga tgttgtggtg ggagagcgtg
     8461 gcgcctttgg gcaggccggt ggtacccgac gtgtactgga tgttgatggg gtcgtccacg
     8521 tccggctccg agccgcgctc cgccaccgcc tccctgggga ccttcccggc gccggacagg
     8581 aacgcccccc acgaggacgc ccagctctct tccgccccca ggaggacgat ccgtcccagg
     8641 gacggggccc gggaccggac ccggtccagc atggccacgt agtcggtgga ccggaagttg
     8701 cgggccgcga ccagcacccc gatccccgag cggcgaagga cgaactcgag ctcgtgggtt
     8761 cgatacgcgg ggttgacgtt caccaggatg gcgccgatca gcgccgtcgc gtactggacc
     8821 agggtccact cggcgcggtt ggcgctccag atacccacgc ggtcgccccg agccaccccc
     8881 agggccagca gcgcgcgagc cacctcctcg ctggcctcct ccagctgggc gaaggtcagt
     8941 cggatgtcct ggtgcggcgc caccagggcc tctacatcgg gatgagcgga agccgagcgt
     9001 cgcagctgat cgccgatggt gcgtcccagc agcggacgat cggcccggcc ggacacgtag
     9061 gacggctcca cgccgctcac ctccctcggc gcaggcatcg tgccgcggtc cggccacgcc
     9121 gaggggggcc gaacggtcgc cggtggacgg ggcgagcggc ccgccgggcg ctaggtcaca
     9181 tcgacgaggc gggacaggtc gaggtcgcgc ccctcgaacc cccggagcca ctggcgcacg
     9241 ttctcgccca ggtggggcac gaagtagccg ccctcctgca gcacgatcgt gcggcggtcc
     9301 agcgcggcca cccgcttgcc cacctcgtgg tacacggggg tggtgagggc caggtcgcag
     9361 atcgggtccc ggccgtaggt gtcgatcccc agcgagatca ccagcgtgtc gcccccgaag
     9421 gcggcgatcg cggcgatggc cgtctccagg tcctgcaggt accgctcgtc cgtgcagccg
     9481 gccggcagcg ggaggttcac gttggtcccc tccccctcgc ccacgccccg ttcgtcggcg
     9541 aacccggtga agtaggggta ggcccggtcc ggatccccgt gcagcgatac gtacaggacg
     9601 tcgccccgct cgtagaagat ctgctgggtc ccgttcccat ggtggtagtc gacgtccagg
     9661 atggcgaccg gctcggtggt gcgcatcgcg aggtggtcgg ccacgatggc cgcgttgttg
     9721 aagaagcagt agccgccgaa ggcccgacgc gccgcgtgat ggcccggcgg acggcagagc
     9781 ccgtacgcca cccgctcacc gcccagcacg acgtcggccg cggtgagggc cacgtccacc
     9841 gcggcgcatg ccgccgcgta cgtcccctcc acgatcgggg tcgccgtgtc gaagcaccac
     9901 gccccgatcc ggccgagcgg cgtggcgggc gccggtcccg ggcccatccc ctcccgcagc
     9961 gccggcagtg ggaaggtgtc ggggaagatc tccgggctcc ccgcccccga ctccacccag
    10021 tccccccaga gctcctcgag gaacccgatc atctccgggt cgtgcaccgc ctcgatcggc
    10081 ccgtggccgt gcccggacgg ctcctccaac tcgaaccccc cgtccgcctc cagcgcggcc
    10141 cggatcgact ccgcccgggc cggggtctcg tacagctcga tggcccgccc gtccgagacc
    10201 tcgtgctggg gggcatggtg gagatgctcg gggctgtaga cgactcgcac gtcggctcct
    10261 cctcccaggg ctcgtcctag ggctcgcgga cgaacaggtc cctcagggcg cggagcgccc
    10321 gccctcgatg gctgatgcgg tccttctcgg cggcgtcgag ctcggccatg gtgcggtccc
    10381 aaccggccgg cacgaagatc ggatcgtacc cgaagccgcc actgccccgg ggcttcgaga
    10441 tcaacgtgcc ctcgcagtgg ccctccgccc agacctcgcg tccgtccggc caggcgaccg
    10501 ccgagacgca ccggtaccga gcggtgcgcc cggccgccgg gatcccggcc agcgctcgca
    10561 ccagcatggc caggttccgc tggtcggtcg cgtgggggcc cgcgtaccgg gccgagcgag
    10621 gaccgggggc gccgcccagc gcgtccacct cgatccccga gtcgtcggcc agggcgggcg
    10681 tctgccgggc ccgtgccacg ccatgggcct tgagcagcgc gttgtccagg tacgtgttcc
    10741 cgctctcctc gatctccggc caggagccgt cgcccggccg ggcccgatcc cactccaccg
    10801 gccagtcggc gcagatcgcc acgatctccc ggatcttccc ggggttgcgg gaggcgatgg
    10861 ccaggcgggg cgggaagctc acgcgcccag ggcctggcgc tggatctcgg tcagctgccg
    10921 gatccccacc tcggccagat cgagcaggga cgacaggtcc tgccgggaga agggagagcc
    10981 ctccgcggtg ccctggatct cgacgagccg tccgccaccg gtcatcacca cgttgaagtc
    11041 cacgtcggcc ccggcgtcct cttcgtagga caggtcgagc acggcacgcc cgtcgaggat
    11101 ccccgccgag accgcggcga cgctgtcggt cagtacctca cgcttcaagg cgccgcgatc
    11161 gcggagcgat cggacggcca gggccagggc gatgtacccg gccgtgatcg aggcggtccg
    11221 ggtcccggcg tccgcctgca ggacgtcgca gtccacccag atcgtgccct cccccatcgc
    11281 ggccaggtcc accaccgagc ggagcgagcg gcccaccaga cgctggatct cctgggtccg
    11341 gcctgacggc cgcccccggc ccacctcgcg cggggtccgc gcggtggtgg ccctcggcag
    11401 catcgagtac tccgcggtga cccatcctcg gccggtcccg tgcaggaacc gcggggcccg
    11461 gtcctcgaag gaggccgcca ccaggacctt ggtccgcccc atctcgagca ggaccgagcc
    11521 ctccgcccac tcctggtacc cgagctccac cttcaccgga cgcagatcgg ccggccctcg
    11581 tccgtcgggg cggtcgctca ctcgcccacc tcgaagcggg aaccctccac cgcgacgtcc
    11641 accgggccgt cgtacacatc ggccgcctcc gagcgcgagc gctcccggtc gtgggtgggg
    11701 agcaggtgtg tgagcaccag tcgccccacc cctccggcct tggcgtaccc gccggcctgg
    11761 cgggcggaca ggtggaaggg ctgcagttcg tcggcgtcct ggtacgtcgc ctcggagagg
    11821 aacaggtcgg catcccgggc cagatcgacg gccgcggggg tgggtccggt atcgcccgtg
    11881 taggccagcg cggagccgtt ggactcgatc cggaagccga gcgccggcag ccccaggtgg
    11941 gacatcgcgc tggcggagac ccggaacggc cccacctcga acccgtcctc gggcgagacc
    12001 tcgtggacgt cgaagccgat gcggaagccc tcgatgctct cctcggagag cagcgccacc
    12061 acccggtcgc cgaacccctt ggggatgtac aggggcatgc cgttcgcgcc catcccggcg
    12121 tagtagcgcg cgtagaagag cgggtagacg tcgacgcagt gatccgcgtg ctcgtgggtg
    12181 atgatcatcg cgtccacgtc ggcgatcgag atgtgcttct gcaggttggc catggtgccc
    12241 gacccggcgt ccacccacag gttgaagcca tcctggcgga tcaggtaccc gctgagcgcg
    12301 gtcccccgat cgggccagct gcccgttgcc cccaggacga tcagctccat gtggcctcct
    12361 cgatgcgccg ggcctcgacg gaggccacgg gaccgatctc cggccccagg aagtggtgcg
    12421 ccacggcccg gaaccgctcc gggtcgccgg tggtcaggaa ctcgtgcacg ggcggggcac
    12481 cgctcgggcg gagcaggtcg cgctccacga gggtcgcgta gacgtccttg gccgtctcct
    12541 cggccgacga caccagcacc acctcgttgc ccatgaccat ctggatcagg cccgacagca
    12601 tcgggtagtg cgtgcagccc aggaccaacg tgtcgacccc ctcctcccgc agcggctcaa
    12661 gatactcctt cgcggccgcc agcaactcgg ggctggaggt atcgcccgcc tcgacgtgtt
    12721 ccacgaagag gggacaggcc cgcgagaaca gccggacctc gggttccgcc gctcccaccg
    12781 cgcgatcgta ggcgccgctg gccaccgtgg cctcggtgcc gatcaacccg acccgccggt
    12841 tccgcgtggt ccggaccgcc gcgcgggctc ccgggtcgat caccccgacc accggaaccg
    12901 ccgccgcatc ggtgacctgc cggatcgcgg ccacctcgat cgagttgcag gccaccacga
    12961 gcatcttcac gtcccgggag accaggtagc cggcggtctc cagggcgaag gaccggatct
    13021 gctccagggg acggggcccg taggggaacc tggcgttgtc gcccacgtag aggaccggct
    13081 cgttcggcag gaggtcgagc acggcccggg ccaccgtgag cccgcccacc ccagaatcga
    13141 acac
//
LOCUS       DASQQQ010000376         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_104166, whole genome shotgun sequence.
ACCESSION   DASQQQ010000376 DASQQQ010000000
VERSION     DASQQQ010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104166"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1413
                     /locus_tag="VE646_14200"
     CDS             148..1413
                     /locus_tag="VE646_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HZD81173.1"
                     /translation="MLHSLVTELFPIPRSLTGEGVRETLRRLREIVPLEIREVPSGTR
                     VLDWTVPKEWNVREAWIRDPRGRTVVDFRDCNLHVVGYSVPVHARLPRDELVRHVHTL
                     PDHPDWIPYRTSYYDESWGFCMAHRQLEALEQGEYEVRIDASLEDGGLTYGECYLTGE
                     LDEEILISCHVCHPALANDNLSGIAVATYLAKGLHERPRRYSYRFLFVPGTIGSIAWL
                     ALNEGALRRIRGGLVLAGVGDPGGLTYKRSRRGDAEIDRAAAHVLRHSGEEHDVQEFI
                     PYGYDERQYCSPGFDLPVGCLMRTPWGTYPEYHTSADDPGLVRPESLAGSLAVCRAIV
                     EVLEGNRTYLNLSPKGEPQLGRRGLYDRVGGRVDRKDRILALLWVLNLSDGGQDLLAI
                     ADRAGMPFGRVRWAADALLDAGLLEERRP"
     gene            1410..>2497
                     /gene="lhgO"
                     /locus_tag="VE646_14205"
     CDS             1410..>2497
                     /gene="lhgO"
                     /locus_tag="VE646_14205"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HZD81174.1"
                     /translation="MTHDFAVVGGGLVGLAVARELTGRYPGTGVLVLEKEDRWAAHQS
                     GHNSGVLHSGVYYKPGSLKARLCRAGHTAMLDFCRQRGIPHRVTGKVVVATEHRELGA
                     LAELERRGRANGLEVTRLGPAELREIEPHAAGIAALRVPEAGVVDFPAVADALAAVVA
                     ERGGDLHLGSEVRALSADDGRVTIETEPETFRARALVNCAGLQGDRIAELMGVRPPAR
                     IVPFRGEYWELRPERSDLVRALIYPVPDPRFPFLGVHFTRAIDGTVHAGPNAVLSLKR
                     EGYRRSDVDPGDAAEVLAYAGFWRFVARFWRTAASEARRSLSGRAFVRSLQRLVPEVV
                     RADVLRGGSGVRAQALARDGRLVDDFLL"
BASE COUNT          324 a          832 c          985 g          356 t
ORIGIN      
        1 gtgcaaccgg tcttccggcc ttacaactga ttgagcgagg ggagcgtggg ccgcaccgcg
       61 tctcgggacc tgagatccgt gcgccccggg gacccggacg ggtcgtcgga gggcgccgtc
      121 gcagccggag cgctcgccgc cggcgacgtg ctccatagtc tggtaaccga gctcttcccg
      181 atcccccgta gcctgacggg tgagggggtg cgggagaccc tgagacgcct ccgcgagatc
      241 gtcccgctcg agatccggga agtacccagc gggaccaggg tgctggactg gacggtgccg
      301 aaggagtgga acgtccgcga ggcctggatc cgggaccccc ggggccggac cgtcgtggac
      361 ttccgggact gcaacctgca cgtggtgggc tacagcgttc cggtgcacgc ccggctcccg
      421 cgcgacgagc tggtccggca cgtccacacg ttgcccgacc acccggactg gatcccctac
      481 cggacctcct actacgacga gagctggggc ttctgcatgg cgcaccggca gctggaggcg
      541 ctggagcagg gcgaatacga ggtgcggatc gacgcgtcgc tggaggacgg gggcctgacg
      601 tacggggaat gctacctcac aggggagctg gacgaggaga tcctgatctc gtgccacgtg
      661 tgccatcccg ccctggccaa cgacaacctg tcgggcatcg cggtggccac gtacctggcg
      721 aagggcctcc acgagcggcc ccgccgttat tcctaccggt ttctgttcgt gcccgggacg
      781 atcggatcga tcgcctggct ggccctgaac gagggggcgc tgaggcggat ccggggcggc
      841 ctggtcctgg ccggcgtcgg cgaccccggc ggcctgacgt acaagcggag ccggcgcggc
      901 gatgcggaga tcgaccgggc ggccgcgcac gtgctgaggc actcgggcga ggagcacgac
      961 gtccaggagt tcatccccta cggctacgac gagcggcagt actgctcgcc gggcttcgac
     1021 ctgcccgtcg gctgtctcat gcggacgccg tgggggacct atccggaata ccacacgtcc
     1081 gcggacgacc cgggcctggt gcggcccgag agcctggcgg ggtcgttggc ggtctgtcgc
     1141 gccatcgtcg aggtcctcga ggggaaccgc acgtacctga acctcagccc gaagggcgag
     1201 ccacagctgg gccggcgggg cctctacgac cgcgtgggcg gtcgggtgga tcggaaggac
     1261 cggatcctgg ccctgttgtg ggtcctgaac ctgtccgacg ggggccaaga tctgctggcg
     1321 atcgccgacc gggccgggat gccgttcggg cgggtccgct gggcggcgga cgccctgctg
     1381 gacgccggtc tgctggagga gcgacgtccg tgacccacga cttcgccgtg gtcggcggcg
     1441 gcctggtcgg cctggccgtc gcccgggagc tgacggggcg gtatccgggg accggcgtgc
     1501 tggtcctgga gaaggaggac cggtgggccg cccaccagag cgggcacaac agcggcgtgc
     1561 tgcactcggg cgtctattac aagcccggca gcctgaaggc gcggctgtgt cgcgcgggcc
     1621 acacggcgat gctggacttc tgccgccagc gcgggatccc gcaccgggtc accgggaagg
     1681 tggtcgtggc gaccgagcac cgggagctgg gcgcgctggc ggagctggag cggcggggcc
     1741 gggcgaacgg gctggaggtg acgcggttgg gtccggcgga gctgcgggag atcgagccgc
     1801 acgccgcggg gatcgccgcg ctccgggtcc ccgaggccgg ggtagtggat ttcccggcgg
     1861 tcgcggacgc gctggccgcg gtcgtggcgg agcgcggcgg cgatctccac ctggggtcgg
     1921 aggtccgggc cctgtcggcg gacgacgggc gcgtcacgat cgagacggag ccggagacgt
     1981 tccgggcgcg tgcgctggtc aactgcgcgg gcctccaggg cgaccggatc gccgagctga
     2041 tgggcgtgcg gccgcccgcc cgcatcgtgc cgttccgggg cgagtactgg gagctgcggc
     2101 cggagcgcag cgatctggtc cgggcgctca tctaccccgt gccggacccc cgcttcccgt
     2161 tcctgggcgt ccacttcacc cgcgccatcg acgggacggt ccacgccggc ccgaacgccg
     2221 tgctgagcct gaagcgggag gggtaccggc gctcggacgt cgatccgggc gacgcggccg
     2281 aggtcctggc ctacgccggg ttctggcggt tcgtggcgcg gttctggcgt accgccgcat
     2341 cggaggcccg ccgctcgctg agcgggcgcg ccttcgtccg cagcctgcag aggctcgtgc
     2401 cggaggtggt tcgggcggat gtgctccggg gcggctccgg ggtccgcgcc caggcgctgg
     2461 cccgggacgg ccggctggtg gacgacttcc tgctgat
//
LOCUS       DASQQQ010000377         3211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_384199, whole genome shotgun sequence.
ACCESSION   DASQQQ010000377 DASQQQ010000000
VERSION     DASQQQ010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384199"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VE646_14210"
     CDS             <1..1053
                     /locus_tag="VE646_14210"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZD81175.1"
                     /translation="VDRLNRRWLMVVCDGARAVLCLGFFLVQGSEMVWLAYVLLALIS
                     SFTAAFDPATEASLPNLVGPRDLVTANALAGSLWGTMLAVGAALGGIVAAVLGRDAAF
                     LIDAASFALSGALIARIRRSFSEEREEEGQHPGIVAASVETARYARKDHGVLALLSVK
                     AGFGLGAGVLVLISVFAQDVFHREEIGIGILMSARGVGALIGPFAGRRFLGPNDRRLF
                     SAIATALVVFGIGYAALGLTPSLALASVAVGVAHLGGGAQWTLSSYGLQRAVPDRIRG
                     RIFAFDGALITLTLTISSIVTGWSANRFGPRPTAIGLGAVAVAYAALWTWFTTDVRRT
                     ALWGQPDALGADPGAD"
     gene            complement(1050..1760)
                     /locus_tag="VE646_14215"
     CDS             complement(1050..1760)
                     /locus_tag="VE646_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZD81176.1"
                     /translation="MIWLWIVLGIVVLAAIGAVVMYNRFISQKQLINDSWANIDTELR
                     RRYDLIPNLVETVKGYAAHERGVFDEVTRARAAAVSATGTPAEQAAAEGPFVAALRQL
                     FAVAENYPELKANQNFLALQAELSNTEDRLQTARRFYNANVRDYNRRVQAFPSNMMAN
                     MFNFREAEFFEIPDAMREAGPPQVNFTQPGPSVSFGEPGAPPAERSAGPGMPSGQGPS
                     AGSGPVPPPPPPMPPQGG"
     gene            complement(1795..2478)
                     /locus_tag="VE646_14220"
     CDS             complement(1795..2478)
                     /locus_tag="VE646_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81177.1"
                     /translation="MEILVFLLFAAAAGAAAYLSYYLKQKRRQELAGMARQLGLDFSA
                     RDTLGCLALPFALFTKGNGRGTENLLYGTWQGMPVREFDYWYYEESTDSQGRRSKTYH
                     RYSCALTEIQAACPHLTIDRENLFTRLADHLGLGDIEFELDEFNRAFNVKGKDRKFAN
                     DFIDQRMMRWLLQADAAFQFEVCATWLLCFSKRRRPTDLIPLIGTTKGFRDQVPPVVH
                     ELYGLGASR"
     gene            complement(2525..2719)
                     /locus_tag="VE646_14225"
     CDS             complement(2525..2719)
                     /locus_tag="VE646_14225"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HZD81178.1"
                     /translation="MAEPRTNRAAVVWGVFFAVAGLGFLLDRLDVWSIRPRYVLPALL
                     IALGVAVLLGGRRGRASGSS"
     gene            complement(2712..>3211)
                     /locus_tag="VE646_14230"
     CDS             complement(2712..>3211)
                     /locus_tag="VE646_14230"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LiaF domain-containing protein"
                     /protein_id="HZD81179.1"
                     /translation="ILPAVLIALGLALVVAAPRGGSQGGPIALGIVLTLILLAGTVVD
                     VPFRGGVGDRTYRPSTVADHTYELAVGKLTIDLSRSGVPVAVPDHVVIRAHVGVGQLV
                     VVVPARFGSVDVRARAGIGQTDLFGQTQDGFGVEDRSPVTNDAGPLLRMDLSVGIGRV
                     EVRSG"
BASE COUNT          470 a         1121 c         1121 g          499 t
ORIGIN      
        1 gtggaccggt tgaaccgccg ctggctcatg gtggtctgcg acggcgcccg cgccgtcctc
       61 tgcctcggct tcttcctggt ccagggctcg gagatggtgt ggctcgccta cgtgctactg
      121 gccctgatct cctcgttcac cgccgccttc gacccggcca ccgaggcctc cctgcccaac
      181 ctggtggggc cccgtgacct ggtgaccgcc aacgcgctgg cggggtcgct gtggggcacg
      241 atgctcgcgg tgggtgccgc cctgggtggg atcgtggcgg ccgtcctggg tcgggatgcc
      301 gccttcctga tcgatgccgc ctccttcgcc ctctcgggag cgctgatcgc acggatccgg
      361 cgttccttct ccgaggaacg cgaggaggaa gggcaacacc ccggcatcgt ggcggccagc
      421 gtggagacgg ccaggtacgc gcggaaggac cacggagtgc tggccctgct ctcggtgaag
      481 gccgggttcg gtctcggtgc cggggtgctc gtgctgatct cggtgttcgc ccaggacgtg
      541 ttccaccggg aggagatcgg catcggcatc ctgatgtcgg ctcgtggcgt gggagccttg
      601 atcgggccct tcgcgggacg gcgcttcctc ggccccaacg accgccgcct gttctccgcg
      661 atcgccacgg ccctggtggt cttcggcatc ggctatgcgg cgctgggact gacgccttcg
      721 ttggccctcg cctcggtggc ggtcggggtg gcgcatctgg ggggaggcgc ccagtggacg
      781 ctgtcctcct acggcctcca acgggcggtg cccgaccgga tccgggggcg gatcttcgcc
      841 ttcgacggcg ccctgatcac gctcacgctc accatctcga gcatcgtgac cggatggtcc
      901 gccaaccggt tcggccctcg gcccaccgcc atcgggctcg gcgcggtggc ggtcgcgtac
      961 gcggccctct ggacctggtt cacgaccgac gtccgccgca cggcgctctg ggggcagccc
     1021 gacgccctcg gtgcggaccc cggtgccgac taaccgccct ggggtggcat ggggggcggg
     1081 ggcggtggga ccggaccaga cccagccgag ggcccctggc ccgagggcat ccccgggccc
     1141 gcggagcgct cggccggcgg ggctcccggc tcgccgaagg agaccgacgg cccaggctgc
     1201 gtgaagttca cctgcggagg accggcctcc cgcatcgcgt cggggatctc gaagaactcc
     1261 gcttcccgga agttgaacat gttggccatc atgttggagg ggaaggcctg gacccgccgg
     1321 ttgtagtcgc gcacgttggc gttgtagaag cgacgcgcgg tctggagccg gtcctccgtg
     1381 ttcgacagct ccgcctggag ggccaggaag ttctggttcg ccttcagctc cggatagttc
     1441 tccgccaccg cgaacagctg acgcagcgcg gccacgaagg gcccctccgc cgcggcctgc
     1501 tcggccggcg tcccggtggc cgagacggcc gcggcccggg cccgggtgac ctcgtcgaac
     1561 acgcctcgct cgtgcgcggc gtagcccttg accgtctcca ccaggttcgg gatgaggtcg
     1621 taccggcgcc ggagctccgt gtcgatgttc gcccaggagt cgttgatcag ctgcttctgg
     1681 gagatgaacc ggttgtacat gacgacggcg ccgatggccg cgagcaccac gatgcccagg
     1741 acgatccaga gccagatcat gacgggtcct ttctcgggtt catcgcgcga cagcctaccg
     1801 actcgccccc agcccgtata gctcgtggac cacgggcggc acctggtcgc ggaacccctt
     1861 ggtcgttccg atcaggggga tcagatcggt cggacggcga cgcttgctga agcagagcag
     1921 ccaggtggca cagacctcga actggaaggc ggcgtccgcc tgcagcagcc agcgcatcat
     1981 ccgctggtcg atgaagtcgt tcgcgaactt gcggtccttg cccttgacgt tgaaggctcg
     2041 gttgaactcg tcgagctcga actcgatatc ccccagcccc aggtggtcgg ccaggcgtgt
     2101 gaacaggttc tcgcgatcga tggtcaggtg ggggcacgcg gcctggatct cggtcagggc
     2161 gcacgagtac cggtggtagg tcttcgaccg ccggccctgg gagtcggtgg actcctcgta
     2221 gtaccagtag tcgaactcgc gcaccggcat cccctgccag gtgccgtaca gcaggttctc
     2281 ggtgccccgt ccgttgccct tcgtgaacag ggcgaacggc agcgccaggc agcccagcgt
     2341 gtcccgggcc gagaagtcca ggcccagttg gcgagccatc ccggccagct cctgacggcg
     2401 cttctgcttc aggtagtagg acaggtaggc ggcggcaccg gcggccgcgg cgaagagcag
     2461 gaagacgagg atctccatga cccgcgtatc ggaccacccc gcgcgggggt gaagcaacca
     2521 gccgctacga cgagccggag gcgcgacccc ttcgcccgcc gagcaagacg gcgacgccga
     2581 gcgcgatcaa caaggcgggc agcacgtagc ggggtcggat gctccacacg tcgagccgat
     2641 ccagcaggaa gccgaggccg gcgacggcga agaacacccc ccacaccacc gcggcacggt
     2701 tcgtccgggg ttcagccact gcggacctcc acccgaccga tgcccaccga taggtccatc
     2761 cgcaggaggg ggccggcgtc gttggtcaca ggcgaccgat cctccacgcc gaacccgtcc
     2821 tgggtctggc cgaacaggtc cgtctgcccg atgcccgccc gggcgcggac gtcaaccgat
     2881 ccgaacctcg cgggaaccac gacgaccagc tgccccacgc cgacgtgcgc ccggatcacg
     2941 acgtggtcgg gaaccgcgac cgggacgccg ctcctcgata gatcgatcgt cagcttgccc
     3001 acggccagct cataggtgtg atcggcgacc gtgctcggac ggtacgtccg gtcgccgacc
     3061 ccacctctga aggggacgtc gaccaccgtc cccgccagca ggatcagggt gaggacgatc
     3121 cccagggcga tcggaccgcc ctggcttccg ccgcgggggg cggccaccac gagtgcgagc
     3181 ccaagagcga tcaggaccgc cggcaggatc a
//
LOCUS       DASQQQ010000378        11380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_384638, whole genome shotgun sequence.
ACCESSION   DASQQQ010000378 DASQQQ010000000
VERSION     DASQQQ010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11380
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384638"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            396..1490
                     /locus_tag="VE646_14235"
     CDS             396..1490
                     /locus_tag="VE646_14235"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZD81180.1"
                     /translation="MTAILLLVAGIGIVNISARSAPSTSEVGTPAAPGRSGSASLAEV
                     RRAVVNINTFAQSIDSPPGSGNLRPLGAGTGMILTSDGEILTNNHVIRQAASIRVSIP
                     GRSQTYTASVVGAAPSADVALIKIDNVSGLPTVTMADPSTVNIGDRVTALGNALGRGG
                     SPTVTKGVITGMDRSITARDPISSPERLSGLIQTDAKIVPGDSGGPLVDTSGHVVGMI
                     TAGSATQGGGSATVGFAIPTSTAIQLVNKMRAGEASSTILLGERGYLGVAVPPADSTQ
                     AIGIRQRLGSGRDTGAPVVGIQPQGPADQAGMTAPALITSIDGRSIGSADALGPAIHA
                     HKPGDKIQVTWVDQSGTHTATVTLIPGPAV"
     gene            complement(1544..2248)
                     /locus_tag="VE646_14240"
     CDS             complement(1544..2248)
                     /locus_tag="VE646_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81181.1"
                     /translation="MPVPRTVPVYLEVARKRTFAGAIDWPGWSRSGRDEATALGALAD
                     HAARYARALRGTRLGFTAVDDPAAFGVVERAVGDSTTDFGAPSITPEADHRPVDEREL
                     HRMQTILRACWRAFDAAVAGAKGADLRKGPRGGGRELDAIVLHVLSAETAYLSRLGHR
                     HRLDPQGDANGELASLRTAVLDGLVSAVRDGVPEAGPRGGARWTPRYFTRRTAWHVLD
                     HAWEIEDRAGPVGPDR"
     gene            complement(2270..3256)
                     /locus_tag="VE646_14245"
     CDS             complement(2270..3256)
                     /locus_tag="VE646_14245"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZD81182.1"
                     /translation="MDPIDRVRAALALQRPDRPPAAWWGHTYRQEWSAVELAEVTAAR
                     QRSYGWDFVKLQPRACCFAQAWGAEYLPHPRFEQAPVQLHRVVSSPEGWSSVRPIDPG
                     APALQEQVEALRLVVRELGPAIPVIQTIFSPLTVAGYLRGEDKPLAARELREHPDAVT
                     PALAGIAGTLATFAARSLEAGAAGIFFAISGYASTDLLGPEEYRALALPHDRRVLEAA
                     GGAWFNVLHLCGPRLHFPVASALATAAVNWSVHEPGNPSLAEGRTLMGRAVMGGVDHD
                     GTMLEGSAREVADQVRAAVEGTGGTGVLVAPGCSVPPVAPEANLRAVTAPPA"
     gene            3678..6269
                     /locus_tag="VE646_14250"
     CDS             3678..6269
                     /locus_tag="VE646_14250"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZD81183.1"
                     /translation="MAGNVHIRVNERDREAPADPERSLLVWLREELGLTGPKYGCGEG
                     ACGACTVLLDGEPVRSCILPLPEASSRTVTTIEGLAPEGSMHPAERAFAEVGAMQCGY
                     CTPGMVMATAALLQTTPDPNDGEIGEALSGNLCRCCTYPRIVRAVHRAAELSRDATSM
                     GRIPRPGPRADERWPRPRRPWGLTDRADRDWFDVLPPGLVVVLPAETQATGWVPSAGA
                     WIHLDRGGRVTAFIGKVDVGQDNRTALGLRVARELHLPLEQVDLVMGDTDLCPFDPGT
                     FGSRSMPDAGQTLARAAAGAREVLAEIAGQRWGVEPSSLHAVDGSFRRSDTGEELGYG
                     DLVGDGPRVEVLSREPPPTVPAGDPTVRRPVPTIGALEAATGVRGYPTDVVRPGMLHG
                     AVLRPPAMGATPRALDTSAAQMQGGTIVHEDTFVGAVSDDPMAPARALAAVRAEWNLA
                     PQPSEGELVQHLRSHPIQAEGWGGVFEDEYGDLDAALSDAAVRLAATYTTAFIAHVPL
                     EARAAVAEWEDGRVTVWTGTQRPFPVREEVAEALGIPSEDVRVIVPPTGAGYGGKHTG
                     EAAVEAARLARAIGAPVKVRWSREEEFWWGYFRPAAVIDVRSGAHRDGTLTAWEFVNI
                     NSGPAAIGCPYDVPNQRIRFQPADSPLRQGSYRALAATANHFARESHIDELACELGID
                     PLEIRLRNLSDERLGEVFRTAAERAGWPGRGFPRGRGLGIAGGIEKDGRVATVADVRV
                     DPEGRLQILRIVTAFDCGAIVDPDNLTNQIEGATVMGLGGALFERIRFGQGRILNGCM
                     SDYRVPRIGDVPPVEVVLVDRPDLPPAGGGETPIVGVAPAIANAVFAATGRRLRSMPL
                     VPDDVVP"
     gene            6376..7683
                     /locus_tag="VE646_14255"
     CDS             6376..7683
                     /locus_tag="VE646_14255"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZD81184.1"
                     /translation="MSDRAVVIGGGAIGIACAHFLTSAGFAVTVVDRGEVGRGCSFAN
                     AGLIVPGDPYPLPGPGVVREGLRHLARRDGPFAIRPRLDPSLARWLWTFRRSSDGVTY
                     ERGKAALASLSRASLQLYEDLAAAGEIDFGFRRVSLIRVFLSPGWRAPTRTGVRDLQA
                     MGFRASVLEPDELRELEPALAPAVGGGVVVEGQASGDCFAYVRSLAARLRSRGVRILE
                     RRRVHHVVVRDGRACGVSVAETSAGRGWAREEIPADLVVLAAGAWTPALSSALGLRVP
                     IQPATGYSATVPTWSGAPRQPLMVEERHVAITPLGDRVRIGGTLELSGFRTSPDPVRA
                     GAVLRGAGEALAEPPPAQPVDTWFGFRPLTPDDLPLIGWAPGVRALIVAAGHGTLGFT
                     QSPITGKLVAELACGEPTSVPLEPFRPDRFGRRVRAHGSRRSP"
     gene            7694..10879
                     /locus_tag="VE646_14260"
     CDS             7694..10879
                     /locus_tag="VE646_14260"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZD81185.1"
                     /translation="MAACPACGTENPEAARFCNACGERLPEAEIAAEVRKTVTVVFCD
                     VTGSTALGERLDPEALRRVMSRYFATMTEAIERHGGTVEKFIGDAVMAVFGIPSTHED
                     DALRAVRAASEMREALRRLNKELERDHGATLECRIGVNTGEVVAGDASVRQALVTGDA
                     VNVAARLEQAARPGEVLMAEVTHRLVRDAVVADAVEPLELKGKRERIAAHRLLTVHAG
                     AAGVARRLDSPLVGRTRQLAKLRQAFEEVAEERVCHLFTILGPPGVGKSRLLQEALGQ
                     VGEGWIVLRGRCLSYGEGITYWPLAEIVRDAIGPDGSPERGDPTAAIADLVTDDPSGD
                     EVAVRVSQLVGGSIDVAAPPEELGWAVRRFLEAMARARPLAVVLEDLHWAEPQLLDLV
                     DQVADLSRDAPILLACLARPDLLELRPGWGGGKLHATTASLEPLSEQECQRLVENLLA
                     GGSLDRRTRDRIVAAADGNPLFVEETIGMLVDDGLLQKQGETWNATRDLTEVAVPPTI
                     QALLAARLDRLDEIERAVLGRASVMGDVFYVDAVGALGPTGDGSPTARILPLVRRDLL
                     RPEVSDLPGHEAFRFHHALLRDAAYQMLPKEVRADLHERFADWLAATAMTIDTDEFVG
                     YHLEQAHRYLVELGPEDEDARALADRAADRLSAAGTRAFERGDRTAAEKLLRRAVALR
                     SSEDPRRSVDLLTLGWVALDADRATDAREAFSEALDLARSAREERLEAHAELGMATSG
                     WLVDPEGGSVMMADVVDRLLPRFEAWGDEHGSALAYLCRSQIHWNHCRFERARRDCML
                     AVDHARAGGDATVERLAAQNAVAAALLGPTDAEGIRTDLAGFERGVGGLPSSRPLALM
                     TRAVLAAMVGDLEGARSLREQGFDMAKELFGEVPAGGLEAGWQSEMLAGDPRVAEGLA
                     RLQYEQISTYGDVAHASTVAGHRATSLLELGQVEEARRLADECRRTSASDDAVNQVIW
                     RSVVARVLAAEGRFEEASRLIAEAVEWSERTDMVVEQARTRFDQAEIHRLEGRKSDAR
                     TALLAARALCERKGIAVWVERADRRLAELDA"
     gene            10997..>11380
                     /locus_tag="VE646_14265"
     CDS             10997..>11380
                     /locus_tag="VE646_14265"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZD81186.1"
                     /translation="MQVDTGFEGLVTERLRLRRSGPDDADQISAYRSDPRVHRYQGWD
                     RTDPEGVRREIEEMAARAPGEPGGWVQLTVEDRESGRLVGDVGFSPAEGEPAVIKIGY
                     TISPAFQGRGYATEAVRALVDYLFDT"
BASE COUNT         1519 a         3976 c         4304 g         1581 t
ORIGIN      
        1 cgcctcactc gttcttcgac cgcgtttacg atcgctggag ggaggcctgc gatgacgcct
       61 ggaggcgggt cctgcgcttc gtcgaggttg ggtgatctcc gagccggtcc tcagcagctt
      121 cccagcttcg gcgggtacct aaggggtgag gttcccaccg gggggaccgg aagaaggtgt
      181 ccgacgtgaa cgaaccggtc gaacgcgaac cgcggtcctc ggcacccgag ggcgccacac
      241 cggcacgccc catggggtcc gagcccgagc catggcccgg cttccgggcc gggatgccgc
      301 cgcctccggc cccgccccct cgcagggccg ggtcgccgtg ggcgagcgag cccacccctc
      361 ccagccaccg gccccgtcgg gtcttcgtgg ctctgatgac ggcgatcctg ctgctggtgg
      421 cggggatcgg catcgtgaac atctccgccc gcagcgcccc ctccacctcc gaggtgggaa
      481 cccccgcggc tccgggccgg agcggcagcg cttccctggc cgaggtgcgc cgggccgtgg
      541 tgaacatcaa cacgttcgcc cagtcgatcg actcgccgcc ggggagcggg aacctcaggc
      601 cgctgggcgc ggggacgggg atgatcctga cctctgacgg cgagatcctg acgaacaacc
      661 acgtgatccg ccaggcggcc agcatccgcg tatcgatccc cgggcgctcc cagacctaca
      721 cggcctcggt ggtgggagcg gctccctcgg cggacgtggc cctgatcaag atcgacaacg
      781 tgtccggtct gcccacggtg accatggcgg atccatccac cgtgaacatc ggcgaccggg
      841 tgaccgccct ggggaacgcg ctgggccggg gtggctcgcc caccgtgacc aagggggtga
      901 tcacggggat ggatcgcagc atcaccgctc gagatccgat ctcctcaccc gagcggctct
      961 cgggcctgat ccagaccgat gcgaagatcg tgcccggtga ctccggaggt ccgctggtcg
     1021 acacatccgg ccacgtggtc ggcatgatca cggccggatc cgcaacccag ggcggtgggt
     1081 cggccaccgt cgggttcgcc atccccacca gcaccgcgat ccagctggtg aacaagatgc
     1141 gggccggtga ggccagctcg accatcctgc tcggtgagcg cgggtacctg ggcgtggccg
     1201 tgccccccgc ggactccacg caggcgatcg gcatccggca gcgcctgggt tcggggagag
     1261 atacgggcgc gccggtggtg ggcatccagc ctcagggtcc tgcggatcag gccgggatga
     1321 ccgctccggc cctcatcacc tcgatcgacg ggcggtccat cggctcggcc gacgcgctgg
     1381 gcccggcgat ccacgcccac aagccgggcg acaagatcca ggtgacctgg gtggatcaga
     1441 gcgggacgca taccgcgacc gtcacgctca tcccgggccc cgcggtctga gcggcgaccc
     1501 ccctgccaca gaccggcgcg cggttcgacc cgtcggcccg ctctcagcgg tcgggcccga
     1561 cgggtcccgc gcgatcctcg atctcccagg cgtgatccag cacgtgccag gccgtccgcc
     1621 gggtgaagta ccgcggggtc catcgagcgc caccgcgggg gccggcctcg ggcaccccgt
     1681 cccggacggc cgagacgaga ccgtcgagca cggcggtccg gagcgatgcc agctccccgt
     1741 tcgcgtcgcc ctgcgggtcg aggcgatggc gatgccccag ccggctcagg tacgcggtct
     1801 cagcgctcag cacatgcagc acgatggcat ccagctcccg gcccccgccc cgcggcccct
     1861 tccgtaggtc ggcccccttg gcgcccgcaa ccgcggcgtc gaaagctcgc cagcaggcgc
     1921 ggaggatcgt ctgcatccgg tggagctcac gttcgtccac tgggcggtga tcggcctcgg
     1981 gggtgatgga cggcgcaccg aagtccgtgg tgctgtcccc gaccgcgcgc tcgaccacgc
     2041 cgaaggcggc cgggtcgtcc accgccgtga accccagcct cgtgcctcgg agggccctcg
     2101 cgtaccgggc ggcgtggtcg gccagggcgc cgagcgccgt ggcctcgtcc ctcccgctgc
     2161 ggctccatcc cggccagtcg atcgctccgg cgaaggtccg tttgcgggcg acctccaggt
     2221 acacggggac ggttcggggg accggcatgg accgaagcct acccgcggct caggccggtg
     2281 gcgcggtgac cgcgcgcagg ttcgcttcgg gagccactgg aggcaccgag cagcccggcg
     2341 ccaccaggac gccggtgccc ccggtcccct cgaccgcggc gcggacctga tcggccacct
     2401 ccctggccga tccctcgagc atggtgccgt cgtggtccac gccgcccatc acggcccggc
     2461 ccatgagggt ccggccctcg gccagggagg ggttgccggg ctcgtggaca gaccagttga
     2521 ccgcggccgt agccagggcc gaggcgaccg ggaagtggag cctcggtccg cacaggtgca
     2581 gcacgttgaa ccaggcgcca cccgctgcct ccaggacccg ccggtcgtgg ggcagggcga
     2641 gggcccggta ttcctcgggc ccgagcagat cggtggaggc gtaccccgag atcgcgaaga
     2701 agatccctgc ggcgccggcc tccagcgatc gggcggcgaa cgtcgccagc gtgccggcga
     2761 tcccggccag cgccggggtc accgcgtccg ggtgctcgcg cagctcccgc gccgcgagcg
     2821 gcttgtcctc accgcgcagg tacccggcca cggtcagcgg ggagaagatc gtctggatca
     2881 cggggatcgc ggggccgagc tctcggacca ccaagcgcag cgcttccacc tgctcctgca
     2941 gggccggagc gccggggtcg atcggacgga ccgaggacca tccctccggg gaggaaacca
     3001 cccggtgcag ctgcacgggc gcttgctcga accgcggatg ggggaggtac tcggcgcccc
     3061 atgcctgcgc gaagcagcag gcgcggggct ggagcttcac gaagtcccac ccgtaggagc
     3121 gctgcctggc cgcggtgacc tcggccagct cgaccgcaga ccactcctgg cggtaggtgt
     3181 gcccccacca cgcggcgggc ggccggtccg gccgctgtag ggccagggct gctcggacgc
     3241 gatcgatggg atccatgccc tccgagtcta gggcgagctc gacgatcaga ggtcaagccg
     3301 ctcgcctggc gcgtcgggtg ttctggaccc gaacgcggcg gacgcgggag gaggtcgagc
     3361 gacgggcgcg ggcggtggca ccggtcgacg gccagcgttc cccacgcagg cagggcccgc
     3421 agaggaagat cgggcctcgg ggggccaggt cgttcacctc gccgcatcgg gcgcaggaca
     3481 cgccggcgcg gtacacgacg tcgcgcggat cgccaccccc gtatgggacg ctcatcgtct
     3541 gctcgctcac ccctgtgagg ttccccgaac cgtgcaccgc aaacgcgttg tcgtggcccg
     3601 cgaggaggct ctcggaccgc tgggatactc gttgggagga tcggttcccc gatgtcggat
     3661 ctccgaggag gtcgctcatg gcgggcaacg tgcacatccg ggtcaacgag cgcgaccgcg
     3721 aggcgccggc cgatcccgag cgcagcctgc tggtctggct acgcgaggag ctgggcctga
     3781 ccggtccgaa gtacgggtgc ggggagggcg cgtgcggagc gtgcacggtc ctgctggacg
     3841 gcgagccggt ccgctcctgc atcctcccgc tgcccgaggc ctcgagccgg acggtcacca
     3901 cgatcgaggg tctggccccc gagggatcga tgcatccggc ggagcgcgcc ttcgccgagg
     3961 tcggggcgat gcagtgcggg tactgcaccc caggcatggt gatggccacc gctgcgctcc
     4021 tccagacgac cccggacccg aacgatggag agatcggcga agccttgtcc ggcaacctct
     4081 gccggtgctg cacctacccc aggatcgtgc gggccgtgca ccgagccgcc gagctgtccc
     4141 gcgacgcgac ctccatgggt cggatccccc ggcccgggcc gagggcggac gagcggtggc
     4201 cccgcccccg ccgtccgtgg ggcctgaccg atcgcgccga ccgggactgg ttcgacgtgc
     4261 tgccccccgg actggtggtg gtcctgccgg ccgagaccca ggcgaccggc tgggtcccct
     4321 cggccggcgc ctggatccac ctcgaccggg ggggccgcgt caccgcgttc atcggcaagg
     4381 tggatgttgg gcaggacaac cggacggcgc tgggcctgcg cgtggcccgg gagctccacc
     4441 tgccgctcga gcaagtcgac ctggtgatgg gcgacaccga tctgtgcccg ttcgaccccg
     4501 ggaccttcgg gagccgctcc atgcccgatg ccggccagac gctggcgcgg gccgccgccg
     4561 gggcgcggga ggtgctggcc gagatcgcgg gccagcgctg gggcgtggag ccctcctcgc
     4621 tgcacgccgt cgatgggtcg ttccgccgga gcgacacggg agaagagcta gggtacgggg
     4681 atctggtcgg cgatggccct cgggtcgaag tcctctcccg cgagccgccc cccaccgtgc
     4741 cggcggggga cccgaccgtc cgccgtccgg ttccgaccat cggcgccctg gaagcggcca
     4801 ccggcgtccg tgggtacccc acggacgtgg tccgccccgg gatgctgcac ggcgcggtgc
     4861 tgcggccacc cgccatgggg gcgacgccgc gcgccctcga cacgtcggcc gcccagatgc
     4921 agggggggac gatcgtgcac gaggacacgt tcgtgggggc ggtctcggac gacccgatgg
     4981 cgccggcccg agcattggcc gccgtgcgcg ccgagtggaa cctggcgccc cagccatcgg
     5041 agggggagct ggtccagcac ctgcgttccc accccatcca ggccgagggc tggggcgggg
     5101 tgttcgagga cgagtatggg gacctcgacg ccgcgctttc cgacgccgcg gtccgcctgg
     5161 cagccacgta caccacggcc ttcatcgccc acgtcccgct cgaggcccgg gccgcggtcg
     5221 cggagtggga ggacgggcgc gtcacggtgt ggaccgggac ccagcgtccg ttcccggttc
     5281 gcgaggaggt ggccgaggcg ctcggcatcc cgtccgagga cgtccgggtg atcgtcccgc
     5341 ccaccggtgc cggctacgga ggcaagcaca ccggcgaggc cgcggtggag gcggcgcgcc
     5401 tggcccgagc catcggtgct ccggtcaagg tccggtggag ccgtgaggag gagttctggt
     5461 ggggctactt ccgccccgcc gcggtcatcg acgtgcggag cggtgcgcac cgggacggga
     5521 ccctcaccgc ctgggagttc gtgaacatca actcggggcc ggcggcgatc gggtgccctt
     5581 acgacgtgcc gaaccagcgg atccgcttcc agccggcgga ctccccgctg cggcaggggt
     5641 cgtaccgggc gttggccgcc accgcgaacc acttcgcgcg ggagtcccac atcgacgagc
     5701 tcgcgtgcga gctggggatc gacccgctcg agatccgact gcggaacctc tccgatgagc
     5761 gcctggggga ggtcttccgg accgccgccg agcgcgcggg gtggcccggg cggggcttcc
     5821 cacggggccg ggggctgggg atcgccgggg gaatcgagaa ggacggccgg gtggctacgg
     5881 tggccgacgt ccgggtcgac cccgaggggc gcctccagat cctgaggatc gtgaccgcgt
     5941 tcgactgcgg cgcgatcgtc gatccggaca acctcacgaa ccagatcgag ggagccaccg
     6001 tgatggggct cggcggggcg ctgttcgagc ggatccggtt cgggcagggc cggatcctga
     6061 acggctgcat gtcggactac cgcgtgccgc ggatcgggga tgtgccgccg gtcgaggtgg
     6121 tgctcgtgga ccgtcccgac ctgcccccgg ccggcggcgg ggagacgccg atcgtgggcg
     6181 tggcgcccgc catcgccaac gccgtcttcg ccgccaccgg acgacgcctg cgctcgatgc
     6241 cgctggtccc cgacgacgtg gtgccgtagc cccatcggcc ccgcacgttc ccggagccgg
     6301 atcaggacct ccgtcgccgg ctcgatcccg tgcccgacgg gtcggccgca ccgtgcactg
     6361 gcgtaggctc gggtggtgag cgatcgcgcg gtcgtgatcg gcggcggagc catcggcatc
     6421 gcctgcgccc acttcctcac ctccgcggga ttcgccgtca ccgtcgtgga ccggggcgag
     6481 gtcggccggg gctgctcctt cgcgaacgcc gggctcatcg ttcccgggga tccgtacccc
     6541 ctccccgggc cgggggtcgt gcgggagggc ctgcgacacc tggcccgacg ggacggtcca
     6601 ttcgccatcc gtccgcgcct ggatccatcg ctggctcgat ggttgtggac cttccgccgc
     6661 tccagtgacg gggtcacgta cgagcgcggg aaggcggcgc tcgcgtccct cagccgggcc
     6721 agcctgcagc tgtacgagga cctggcggcg gcaggcgaga tcgacttcgg cttccggcgt
     6781 gtctcgttga tccgcgtgtt cctctcgccg ggctggagag cgccgacccg gacgggggtg
     6841 cgggatctgc aggcgatggg gttccgggcc tcggtgctgg aaccggacga gctgcgggag
     6901 ctggagccgg cgctggcacc cgctgtgggc ggtggcgtcg tggtcgaggg gcaggcctcc
     6961 ggtgactgct tcgcctacgt gcggagcctg gccgctcggc tgaggtcccg aggcgtccgg
     7021 atcctcgagc gccggagggt ccaccacgtc gtggttcggg acggccgggc ctgcggggtg
     7081 tccgtggccg agacgagcgc agggcgcggg tgggcacgcg aggagatccc cgcggacctg
     7141 gtcgtgctgg cggccggggc gtggaccccc gccctgagct cagcgctcgg tctccgggtc
     7201 cccatccagc cggccacggg ctacagcgcc accgttccca cctggtcggg ggcgccccgg
     7261 caaccgctca tggtcgagga gcgccacgtc gcgatcacgc ccctgggaga tcgagtgcgg
     7321 atcgggggga ccctggagct gtcggggttc cggacgtcac ccgatcccgt ccgcgccggt
     7381 gcggtgctcc gcggggccgg tgaggcgctg gcggagcccc ctcccgcgca gccggtggac
     7441 acgtggttcg gcttccggcc cctgaccccc gacgacctcc ccctgatcgg atgggctccg
     7501 ggagtgcggg cgctgatcgt ggcggcaggc cacgggacgt tggggttcac gcagtcgccc
     7561 atcacgggca agctggtcgc cgagctggcg tgcggcgaac cgacatcggt ccccctggag
     7621 ccgttccgcc cggatcgctt cggacgccgc gttcgcgctc acgggagccg ccggtccccc
     7681 tagacttcgg gtcatggctg cttgccctgc atgcggcacg gagaaccctg aggctgcgag
     7741 gttctgcaac gcctgcggcg agcggctgcc cgaggcggag atcgctgccg aggtgcgcaa
     7801 gaccgtgacg gtggtgttct gcgacgtcac cgggtcgacc gcgctcgggg agcgcctgga
     7861 ccccgaggcg ttgcgccggg tgatgagccg gtacttcgcc accatgacgg aggccatcga
     7921 gcgtcatggg gggacggtgg agaagttcat cggggacgcc gtgatggcgg tgttcggcat
     7981 cccctcgacg cacgaggacg atgccctgcg agcggtccgg gcggcgagcg agatgcgcga
     8041 ggcgctccgt cggctgaaca aggagctgga gcgcgaccac ggcgccaccc tcgagtgccg
     8101 gatcggtgtc aacaccggcg aggtggtggc gggtgacgcg agcgtgcggc aggctctggt
     8161 caccggggac gccgtcaacg tggcggctcg cctggagcag gccgctcggc ccggcgaggt
     8221 cctgatggcg gaggtcacgc atcggttggt ccgagacgcc gtggtggcgg atgcggtgga
     8281 gcccctggag ctgaagggga agcgcgagcg catcgcggcg caccggctgc tcaccgtcca
     8341 cgccggcgcg gcgggcgtgg cccgccggct ggactcgccc ctggtcggcc gcacccgcca
     8401 gctcgccaag ctccgccagg ccttcgagga ggtggccgag gagcgggtgt gccacctctt
     8461 cacgatcctc ggccctccgg gggtgggcaa gtcgcggctc ctgcaggaag cgctgggcca
     8521 ggtgggtgag ggctggatcg tgctccgggg gcgatgcctc tcgtacggag aaggcatcac
     8581 ctactggccc ttggccgaga tcgtccggga tgcgatcggg ccggacggct ccccggagcg
     8641 gggcgatccc acggccgcga tcgcggatct ggtgaccgac gatccttccg gggacgaggt
     8701 ggcggtccgc gtctcgcagc tggtgggagg ctccatcgac gtggccgctc cccccgagga
     8761 gctcggatgg gccgtccgcc gcttcctgga agccatggcc cgagctcgtc cgctggcggt
     8821 ggtcctcgag gacctccact gggcggagcc ccagctcctg gatctggtgg accaggtggc
     8881 cgacctcagt cgcgacgccc cgatcctgct ggcgtgcctg gcccgccccg acctgctgga
     8941 gctgcggccg gggtggggtg gcgggaagct ccacgccacc accgcgtcgt tggagccgtt
     9001 gtcggagcag gagtgccagc gcttggtgga gaacctgctg gccggtgggt ccctggaccg
     9061 acggacccgg gatcggatcg tagcggcggc cgacggcaac ccgctgttcg tggaggagac
     9121 gattgggatg ctggtcgacg acgggctcct gcagaagcag ggagagacgt ggaacgccac
     9181 gcgagacctg accgaggtcg cggtgccgcc cacgatccag gcgctcctcg cggcccgtct
     9241 ggaccgtctc gacgagatcg agcgggcggt gctgggacgg gcgtcggtga tgggcgatgt
     9301 cttctacgtg gacgcggtcg gcgccctcgg accgaccggc gacggcagtc ccacggcgcg
     9361 catcctgccg ctcgtccgtc gagacctcct ccgccccgag gtctccgacc tgcccgggca
     9421 cgaggcgttc cgcttccacc acgccctgct gcgcgacgcc gcctaccaga tgctgcccaa
     9481 ggaggtccgt gccgacctgc acgagcgctt cgccgactgg ctcgccgcca ccgccatgac
     9541 catcgacacc gatgagttcg tgggctacca cctcgagcag gcccatcggt acctggtgga
     9601 gctcggtccc gaggacgagg acgcccgggc gctcgcggat cgggccgccg atcggttgtc
     9661 ggcggcgggg acccgagcct tcgaacgggg cgatcgcacg gccgccgaga agctcttgcg
     9721 ccgagcggtc gccctgcgct cctcggagga tccgcgtcgg tcggtggacc tgctgacgtt
     9781 ggggtgggtc gctctggacg ccgaccgcgc caccgatgcg cgggaggcgt tctcggaggc
     9841 cctcgaccta gcccgctccg cccgggagga gcggctcgag gcccacgccg agctggggat
     9901 ggcgacatcc ggatggctgg tcgaccccga gggcggctcc gtgatgatgg cggatgtggt
     9961 cgaccggctc ctgccacggt tcgaggcatg gggggacgag cacgggtcgg cgctcgccta
    10021 cctctgccgg tcacagatcc actggaacca ctgccggttc gagcgggcgc gccgggactg
    10081 catgctcgcc gtggaccacg ctcgggccgg gggcgacgcg accgtcgagc ggctggcagc
    10141 gcagaacgcg gtggccgctg ccctgctggg gcccacggac gcagaaggta tccgaaccga
    10201 cctggccggg ttcgagcggg gtgtcggagg gcttccctcc tcccggccgc tcgcgctcat
    10261 gacccgggcg gtgctcgcgg ccatggtcgg cgatctggaa ggggcgcgtt ccctccggga
    10321 acagggtttc gacatggcca aggagctgtt cggcgaggtt ccggcgggag gcctggaagc
    10381 cggctggcag tcggagatgc tggccggaga tccccgggtg gccgagggac tcgcgaggct
    10441 gcagtatgag cagatctcga cgtacggtga cgtggcccac gcctccaccg tggctggtca
    10501 ccgtgcgacc tcactcctgg agctcgggca ggtggaggag gcccgccgac tggcggacga
    10561 gtgtcggcgg accagtgcca gcgacgacgc tgtcaaccag gtgatctggc gcagcgtcgt
    10621 ggcgcgggtg ctggctgccg aggggaggtt cgaggaggct tcgaggctga tcgccgaggc
    10681 ggtcgagtgg agcgagcgca cggacatggt ggtagagcag gccaggacgc gcttcgacca
    10741 ggcggagatc caccggctgg aggggcggaa gtcggatgcg cggaccgcgc tcctggccgc
    10801 ccgggcgctc tgcgagcgca aggggatcgc cgtgtgggtc gaacgcgccg atcggcgact
    10861 cgcggagctc gacgcctgat cccgggcctc gcgcactgca ggtgatgtag ggcgcgtggc
    10921 tcaaggacgg cgtgcagacc ggcgagcaca gggcacggac ggactgggat gttggcatgg
    10981 gggtaccctt gcctcgatgc aggtcgatac cgggttcgag gggctggtga ccgagcggct
    11041 gcggctgcgg cgatccgggc ccgatgacgc cgaccagatc tcggcctatc gcagcgatcc
    11101 ccgcgtgcac cggtaccagg gatgggatcg caccgatccc gagggcgtgc ggcgtgagat
    11161 cgaggagatg gcggcgcgcg cacccgggga gccgggcggc tgggtgcagc tcaccgtcga
    11221 ggaccgggag agtgggcgac tggtgggcga cgtgggcttc agcccggccg agggagaacc
    11281 tgcagtcatc aagatcggct acacgatctc gccggccttc cagggccgcg gctacgccac
    11341 cgaggccgta cgtgcgctgg tcgactacct cttcgacacc
//
LOCUS       DASQQQ010000379         2676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_106237, whole genome shotgun sequence.
ACCESSION   DASQQQ010000379 DASQQQ010000000
VERSION     DASQQQ010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106237"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VE646_14270"
     CDS             <1..261
                     /locus_tag="VE646_14270"
                     /inference="COORDINATES: protein motif:HMM:NF014787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HZD81187.1"
                     /translation="LTFDRERVQVDGSLQDVGRGAAAIEAKYEGEALTVAFNPTYLSD
                     GVGAVEDNEVVIEVRDGLKPALVHGSERRGFLYLLMPVRLPA"
     gene            641..1771
                     /gene="recF"
                     /locus_tag="VE646_14275"
     CDS             641..1771
                     /gene="recF"
                     /locus_tag="VE646_14275"
                     /inference="COORDINATES: protein motif:HMM:NF000822.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HZD81188.1"
                     /translation="MYVERLELVDFRNHSMVALDLLPGASVLVGPNGVGKTNVLEAIG
                     YLTTLGSHRSGPESSLVRVGASAAIVRAAVVREGHTVVVDLEIRPGAGARARVNRAPL
                     PRARDALGIVRATLFAPEDLALVRGDPEDRRRFLDTLAVQRLPRYLALRQDYDRVLRQ
                     RNTLLRSAGGRMPAGTGLATLDVWDEKLAAAGAELWAERLRLIELIRAPVERSYQDLA
                     GQPDRVRMVYVSSVLGDQEVEPDAPRLARTLRERLLADRAREVERGVTLSGPHRDELA
                     LSVRELPARTHASQGEAWSLALALRLGAHRLLAEEGEEPMLLLDDVFAELDRRRRERL
                     ADLALTVEQAIITASVLDELPLHGKATMFHVKPSRITREAAR"
     gene            1830..2165
                     /locus_tag="VE646_14280"
     CDS             1830..2165
                     /locus_tag="VE646_14280"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HZD81189.1"
                     /translation="MSPDPRPIGEEIDRMLRSRGWQQRLVAARLVARWSEVVGPAVAN
                     HCQPRRLEDDGTLTVVADSAAWATQLSYLQATLLERVAAIVGPRVVKQVRVRAGAAAG
                     FGRGARAKP"
BASE COUNT          352 a          885 c         1038 g          401 t
ORIGIN      
        1 ctgacgttcg accgcgagcg cgtgcaggtg gatggcagcc tgcaggacgt cggccgggga
       61 gcggcggcga tcgaggcgaa gtacgaggga gaggcgctca ccgtcgcctt caacccgacc
      121 tacttgagcg acggagtggg agcggtcgag gacaacgagg tggtgatcga ggtccgggac
      181 ggtctgaagc cggcgctggt gcacggcagc gagcggcggg gcttcctgta cctgctgatg
      241 ccggtccgcc tgcccgcatg acgcccgctg gccaggctga agagcccggc cagcgggtcg
      301 ccgtcgattc cggccggcag cgcccaccgt ccatccggcc cggacggtgg gccgccgctc
      361 cttcgcgtcg ggggacaggg gcgcttgcgg tcgattccgg tcccggaggg gccttcgcgc
      421 cgtttcctcc acgtctccgc ctgccgggct cccgccgtct cggcaacgag gtccgcgcca
      481 tgtcaacgcc ggcccatcag cgccaggccc cggcagcccg gtcaccctcg gccggccggc
      541 gttgggccgg gatatcgtgg agccgttcca tgtcctgggc gagcagcgcc gcgagcggga
      601 gcggcgggaa cgccatgaac ccgggcggct gagggggcgc atgtacgtcg aacgtctgga
      661 gctggtggac tttcgcaacc actccatggt cgcgcttgac ctgttgcctg gggcctcggt
      721 gctggtggga cccaatgggg tgggcaagac caatgtgctc gaggccatcg gctacctgac
      781 caccctgggg tcgcaccggt ccgggcccga gtcgagcctg gtgcgcgtgg gggcatccgc
      841 cgccatcgtg cgcgcggcgg tggtgcgaga ggggcacacg gttgtggtcg atctcgagat
      901 tcgacctgga gccggcgccc gggcgcgggt caatcgagca ccgctcccca gagcgcggga
      961 tgccctcggg atcgtccggg cgacgctgtt cgctcccgag gatctcgccc tggtccgtgg
     1021 ggacccggag gaccgtcgcc ggttcctgga cacgctggcc gtccagcggc tgcctcgcta
     1081 cctggcgctc cgtcaggact acgacagggt gcttcgccag cggaacacgt tgctgcgcag
     1141 cgccggcggc cggatgccgg caggtaccgg cctggccacg ctcgacgtct gggatgagaa
     1201 gctggcggct gccggcgcgg agctctgggc ggagcgcctc cgcctgatcg agttgatccg
     1261 cgcccctgtg gaacggtcat accaggacct ggccggccag ccggaccgcg tgcgcatggt
     1321 ctacgtctcc tctgtgctgg gcgaccaaga ggtggagccg gacgctcccc ggctggcccg
     1381 gacgctgcgg gagcgtcttc tggcggatcg tgcccgtgag gtggagcggg gggtcacgtt
     1441 gtcggggccc catcgggacg agcttgccct gtccgtgcgt gagcttccgg cgcggaccca
     1501 cgcctcgcag ggagaggcct ggtcgctggc gctggcgttg cggctggggg cccatcggct
     1561 ccttgccgag gagggcgagg agccgatgct gctcctcgac gacgtgttcg cggagctcga
     1621 ccggcgccgg cgtgagcggt tggcggatct ggcgctgacg gtcgagcagg ccatcattac
     1681 cgcgtccgtg ctggacgagc tgcctttgca cggcaaggcg acgatgtttc acgtgaaacc
     1741 aagccgcatc acgagggagg ctgctcgatg aatcctcggc caccggcggc ttcccaccgc
     1801 gcgtgctcct cagcagggcg ggcgccgtca tgagcccgga tccgaggccg atcggtgagg
     1861 agatcgatcg gatgctgcgg tccagaggct ggcaacagcg gctggtcgca gcccgcctcg
     1921 tggcccgctg gtcggaggtc gtcggcccgg cggtcgccaa ccactgccag ccaaggcggc
     1981 tcgaagacga cggcacgttg acggttgtgg ccgattcagc cgcctgggcc acccaactct
     2041 cctacctcca ggccacgctg ctcgagcgcg tcgccgcgat tgtcggccca cgggtggtca
     2101 agcaggtgcg ggtgcgggcc ggtgccgccg ccggcttcgg ccgtggcgcg cgagcgaagc
     2161 cttgaccgtc ggcggttccg ccggcaaggg gagttgtcgc ggtcccgggg tatgacttcg
     2221 ttgagcgcgg cgctcgtggc gctggcgctc acgtcaatga gactgtccgt gttgctcgga
     2281 ggggccatgg accggggcct gcaggaccgt gaccggagcg ggagccgggg ggttcggggg
     2341 gcgcagcgca gcgcagcccc ccgatcaaca gatcgagggt ggtcgagtgg ctggggagca
     2401 tcaccgacac cctgaatcag tcagaccaga cgggaacggg ccggtgctcc aggcgcggca
     2461 agcaaccgca ccagcttcgg cgtccagcgt gtcgaggagc gtgcttggcg tggcccatcg
     2521 acccaggcga gtcggcggag gcgccgggtg gtgcgcccgg acgcggcgac cgaccgggtt
     2581 ggcgcggagg ttggcggcac gtggggactg tgggccggcg cggaccgtgg gcggccggga
     2641 ccgctggagg gcctcccgac ccgggatgtg ccgcct
//
LOCUS       DASQQQ010000380         4582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_107032, whole genome shotgun sequence.
ACCESSION   DASQQQ010000380 DASQQQ010000000
VERSION     DASQQQ010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107032"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..433
                     /locus_tag="VE646_14285"
     CDS             206..433
                     /locus_tag="VE646_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81190.1"
                     /translation="MMPKMLRGAAALAFASGVAIAILIPEAPLARSVTYGPGIPSTAD
                     NRLGLRLAIVAVALVGSAILLVVSRRARGGS"
     gene            complement(776..931)
                     /locus_tag="VE646_14290"
     CDS             complement(776..931)
                     /locus_tag="VE646_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81191.1"
                     /translation="MAETPDEILRPLADPERLEIVGTWPGDGARQSWPPSSPFPCSSG
                     PAGTSAV"
     gene            957..1886
                     /locus_tag="VE646_14295"
     CDS             957..1886
                     /locus_tag="VE646_14295"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZD81192.1"
                     /translation="MMEATTVLSPIQRRTLDGLIGIRERPSFPPELVPRLRDRIEEAA
                     RACELAEPIWLGKSQLSDLDRCPGLFDAVRAGERSPFAFSARFAAGRLAHKAVELEVA
                     GREERDPHGLVEQAVDRLLEDDAFRSFWEDLDPLRRDEVLMEGAKLLELFRSTLPPLR
                     PMRRELAPSTEWHLRAELLGGAVVLSGTLDLVLGAAAPAEPTRATRLVIDLKTGRAWP
                     EHAEDMRFYALLLALRFGVPPYRVASLYLDSGEWVAEDVDARVLDRAAERVIGAVRSA
                     AASAAGRDRELRPGPYCTWCPRTATCPRSAAPD"
     gene            2048..3289
                     /gene="ccrA"
                     /locus_tag="VE646_14300"
     CDS             2048..3289
                     /gene="ccrA"
                     /locus_tag="VE646_14300"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017215191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HZD81193.1"
                     /translation="MTKDVYEIGELPPIGEVPRQMHAQLVRPDRYGEPRDAIRDEVID
                     VPPIGPHEALVMVMAAGVNFNNVWAARGIPVDVTKTQARWGEPTDFHIVGSDASGIVY
                     AVGPEVTNVKVGDRVIVHAGQWDHDDPWAAAGNDPGLAPSFRVWGYDTCWGSFSQFCK
                     VQAHQCLPKAEHLTWEEAAAPTLTGATAYRMLHGWPPNTVEEGDVVLVWGASGGLGSL
                     AVQLVTLAGARAVGVVSSAEKGEFATSLGAVGYINRKDFDHWGVPPAWDSPEWKSWFD
                     GAKAFGKAIWDVLGERRSPNIVFDHPGQDTIPTSIFVCERGGMVVICAGTTGYDTMVD
                     VRYLWYMQKRYQGSHLFNDEQAAAFNDLVREGKVRTTLGHAYPYGDAGEVHQLMGDGR
                     LPQGNVAVLINAPREGLTDLP"
     gene            complement(3286..>4582)
                     /locus_tag="VE646_14305"
     CDS             complement(3286..>4582)
                     /locus_tag="VE646_14305"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZD81194.1"
                     /translation="PMDEEVRILERALDVFTEIGDRAGLGWTHARLVVPLLMQARHEE
                     GEAAGRTALETLEPLGERPELADALQRLGWFLWRRGRNDEAEPLLRRAVEMAERLGAP
                     LVRAEATQTLGVCLVQMGRTAEGIDMTEEAYRLAKEVGEFSNLMRCYNNLPALVGDYA
                     SDFAKAETVLREGRELAQRAGAKSHQGWITGSLGDVAFKLGRLEESESFQREAVELAV
                     AVGDEPLRGMRLIGLAAALLFRGRLEDARAVLEEAIPIVRANPEPQADLYVPMFQGHL
                     ALVRGDPATAADRFSQTVELLRAFNVDAEPSAFVDLVRALQRAGRAGQAETYRDLSEH
                     GSSPAARANATLVEGLLAESPVEAHRLLADGVGSLEGLGLRIDAARGMVDAGRAMARV
                     GEDPRETFQRARDILVGCGANAFLFELPDASATLDRGSD"
BASE COUNT          632 a         1659 c         1622 g          669 t
ORIGIN      
        1 gctccagggc ctgatcggga ccggtgcccg gcccaccttc ccgcccgagc tggtgccgcg
       61 gctgcgggac cggatcgagg aggccgcccg ggcctgcgag ctggccgagg ccatctggct
      121 gggcaaggca aactagctcg ctaggtacct aagacatctg cctggtccca agcccttgcc
      181 agttcgagag tgagaggatg acgccatgat gccaaagatg ctccgaggtg ctgcggcgct
      241 ggcattcgca tcgggggtcg cgatcgccat cctcattcct gaagcgcctt tggctcgttc
      301 ggtgacctac ggtcccggca tcccgagcac agcggacaat cgcctcggac ttcggctggc
      361 catcgtcgcg gtggccctcg tcggatcagc aatcctgctt gtggtgagcc ggcgggccag
      421 gggaggaagc tgatccacgc ctccgagttc cgcagaggac actggagagc cgaagttgaa
      481 aggctcacgt cctgttcggt ggggggtcgt ggccagaatg ccgaccaagg gccggtaact
      541 cggcggccta cgcaactcca agacgatccg ccgcttggac tccttagcgg agatctcgac
      601 taggcgcccg ttgcggaggg acgcacgcag gacgtcctcc tcgttcccgt ctgcggctcc
      661 catcgcgaca ccggtctagc gaggcggccc cacctttcgg cggtccaccg cagggtctcg
      721 gggtcaggtc ggtaggtccg ccggtcctcc tgcaggcgga ccacgcccgt cgcggtcaga
      781 cggctgaggt gcctgcgggc ccgctgctgc aggggaaggg cgagctcggc ggccagctct
      841 gccgcgctcc gtcgccgggc caggttccga cgatctccag tcgctccgga tcggcgaggg
      901 ggcgcaggat ctcgtcgggg gtctccgcca tgtcggtggc tactcctact atcccgatga
      961 tggaggcaac caccgtcctc tcgccgatcc agcgcaggac cctcgacggg ttgatcggca
     1021 tccgggagcg cccgtccttc ccgcccgagt tggtgccgcg gctgcgggac cggatcgagg
     1081 aggccgcccg ggcctgcgag ctggccgagc ccatctggct gggcaagtcc cagctgagcg
     1141 acctggaccg gtgcccggga ctgttcgatg ccgtgcgcgc cggggagcgg agccccttcg
     1201 ccttctcggc ccgcttcgcc gccggacggc tggcgcacaa ggccgtggag ctggaggtgg
     1261 ccgggcgcga ggagcgcgat ccgcacggcc tggtggagca ggccgtggac cggctgctcg
     1321 aggacgacgc cttccgctcc ttctgggagg acctggaccc gctccgccgg gacgaggtcc
     1381 tgatggaggg cgccaagctt ctggagctgt tccgctccac cctgcctccc ctccggccga
     1441 tgcgccgcga gctcgctccc tccaccgagt ggcaccttcg ggccgagctg ctgggcggcg
     1501 cggtcgtcct gtcaggcacg ctcgacctgg tcctgggggc cgccgccccg gccgagccca
     1561 ccagggccac ccgcctggtc atcgacctca agaccggccg ggcctggccg gagcacgcgg
     1621 aggacatgcg gttctatgcg ctgctgttgg cgctgcgctt cggcgtgcct ccgtatcgcg
     1681 tggcatccct gtacctggac tcgggggagt gggtagccga ggacgtcgat gcccgggtgc
     1741 tggaccgggc cgccgagcgc gtgatcggcg cggtgcgctc ggccgccgcc tccgccgccg
     1801 ggcgagaccg cgagcttcgg cccggcccgt actgcacgtg gtgcccccgg acggccacgt
     1861 gtccgcggag cgccgccccg gactgactct ttcgtccgga ccagcggcgt gagccgtggt
     1921 cgcgatcctg ccctcgaacg cccgggccgc gccgccctgc gccgacggac ggcgcaccgt
     1981 ccgggttgtc accctgcgct ccgtgcggct agcgtccgcc cgaacccctt cctgcggagg
     2041 tgcggacgtg acgaaggacg tgtacgagat cggcgagctt ccgcccatcg gcgaggttcc
     2101 gaggcagatg cacgcgcagc tcgtgcgtcc cgaccgctac ggagagccca gggacgcgat
     2161 ccgcgacgag gtgatcgacg tcccacccat cggcccccac gaggcgctcg tgatggtcat
     2221 ggctgccggc gtgaacttca acaacgtgtg ggcggcccgg gggatccccg tcgacgtcac
     2281 gaagacccag gcccggtggg gtgagcccac ggacttccac atcgtgggct ccgacgcatc
     2341 cggcatcgtc tacgccgtgg gccccgaagt gacgaacgtg aaggtcggcg accgcgtcat
     2401 cgtgcacgcc ggccagtggg accacgacga tccgtgggcg gcggcgggga acgaccccgg
     2461 cctggcccca agcttccggg tgtggggcta cgacacctgc tggggatcgt tcagccagtt
     2521 ctgcaaggtc caagcccacc agtgcctgcc caaggcggag cacctcacct gggaggaagc
     2581 cgcggcaccc accctgaccg gcgctaccgc ctaccggatg ctccacggct ggccgccgaa
     2641 cacggtggag gagggggacg tcgtcctggt gtggggtgcc tccggcggcc tgggttccct
     2701 ggccgtgcag ctggtgaccc tggccggggc gagggccgtc ggcgtggtct cgagcgcgga
     2761 gaagggcgag ttcgcgacgt ccctcggcgc ggttggctac atcaaccgca aggacttcga
     2821 ccactggggc gtgccgccgg cctgggactc gccggagtgg aagtcctggt tcgacggcgc
     2881 gaaggccttc ggcaaggcca tctgggacgt gctcggcgag cgccggagcc cgaacatcgt
     2941 cttcgaccat cccgggcagg acacgatccc cacctcgatc ttcgtctgcg agcgcggggg
     3001 gatggtggtg atctgcgcgg ggaccaccgg ctacgacacg atggtcgacg tccggtacct
     3061 ctggtacatg cagaagcggt accagggctc ccacctcttc aacgacgagc aggcggcggc
     3121 cttcaacgac ctggtccgcg agggcaaggt gcggaccaca ctggggcatg cgtatccgta
     3181 cggggacgcg ggcgaggtcc accagctgat gggcgacggc cggctgcccc agggcaacgt
     3241 ggcggtcctg atcaacgcgc cccgggaggg tctgacggac ctgccctagt ccgaaccgcg
     3301 gtcgagcgtg gccgaggcgt ccgggagctc gaacaggaag gcgttggcac cgcacccgac
     3361 gaggatgtcc cgggcgcgtt ggaaggtctc gcgtggatcc tcgccgacgc gggccatcgc
     3421 ccggcccgcg tccaccatgc cccgggcggc gtcgatccgg aggccgagcc cctccaggga
     3481 cccaaccccg tcggccagca gtcggtgggc ctccaccggg ctctccgcga gcaggccctc
     3541 gaccaacgtg gcgttcgcgc gggcggccgg cgagctcccg tgctcggaca ggtcccggta
     3601 cgtctcggcc tgcccggcac gccccgcccg ctggagcgcc cgcaccaggt ccacgaaggc
     3661 gctgggctcg gcgtccacgt tgaacgcccg caggagctcg acggtctggg agaagcggtc
     3721 ggcggccgtg gccggatcgc cgcggaccag cgccaggtgg ccctggaaca tcggcacgta
     3781 caggtcggcc tggggctcgg ggttcgctcg gacgatgggg atggcctcct cgagcaccgc
     3841 ccgcgcatcc tcgagccggc cccggaacag gagcgccgcg gccagcccga tcaaccgcat
     3901 gccccggagc ggctcgtcgc ccacggcgac ggccagctcg acggcctccc gctgaaacga
     3961 ctcggactcc tccagcctgc cgagcttgaa cgcaacgtcg ccgaggctgc cggtgatcca
     4021 gccctggtgg ctcttcgcac cggcgcgctg cgccagctcg cgtccctcgc ggagcaccgt
     4081 ctcggccttg gcgaagtccg aggcgtagtc gcccaccagg gcgggcaggt tgttgtagca
     4141 gcgcatcagg ttcgagaact cccccacctc cttggccagc cggtaggcct cctcggtcat
     4201 gtcgatcccc tcggccgtgc gccccatctg caccaggcac accccgaggg tctgcgtcgc
     4261 ctccgctcgc accagcggcg cgcccaggcg ctcggccatc tccacggcac ggcgcagcag
     4321 cggctcggcc tcgtcgttcc gcccccgccg ccacaggaac cagccgagcc gttgcagcgc
     4381 gtcggccagc tccgggcgct ccccgagggg ctccagcgtc tcgagcgcgg tccggcccgc
     4441 ggcctcgcct tcctcgtgac gggcctgcat cagcaggggc acgaccaggc gtgcatgggt
     4501 ccaacccagc ccggcccggt cgccgatctc ggtgaacacg tcgagcgctc gttccaggat
     4561 ccggacctcc tcgtccatgg gg
//
LOCUS       DASQQQ010000381         5671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_107478, whole genome shotgun sequence.
ACCESSION   DASQQQ010000381 DASQQQ010000000
VERSION     DASQQQ010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107478"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VE646_14310"
     CDS             complement(<1..350)
                     /locus_tag="VE646_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81195.1"
                     /translation="MELAHLDVLRDRNWPFDRWFRALGYTFRVRSDLDGAQGVLSRLL
                     APFLDRDEPEQVPTYTLTRRLSPGSIQDTSRFRYELFLDGSSIQRVSSPGSMLDWLIT
                     DFCRRAVESTEEYVA"
     gene            complement(984..2141)
                     /locus_tag="VE646_14315"
     CDS             complement(984..2141)
                     /locus_tag="VE646_14315"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014134790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZD81196.1"
                     /translation="MTEPIAHPLGPPKAPAFPEGLPLARPRVAEPAVVGADVEAILRS
                     GVLTNGPFVRRLEARAAEYLGVHHCIAVSSCTSGLMLMLRAAGLSGDVIVPSFTFMAT
                     AHAVAWNGLRPVFADIDPSSLTLDPRSVEKAVGVRTSAVLATHVYGSPCDVEGLQAVA
                     DRHGIRLFFDAAHAFGSLRAGRHVGGFGGAEVFSLSPTKPLVAAEGGIVATDDDVLAE
                     RCRMGRDYGNPGDYDCRFVGLNGRMSELHAAVALASFADLEGRIGERNRIAGWYRDAL
                     DGVPGIRFPSVPGGDRSTYKDLAVLVDPEAFGMDADALSLALAAEGIETRRYYAPPVH
                     RTRAYARSSTNGGLPVTDEASARALCLPLWEGMAMEAVERVAEVLTRAGAR"
     gene            complement(2206..3171)
                     /locus_tag="VE646_14320"
     CDS             complement(2206..3171)
                     /locus_tag="VE646_14320"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81197.1"
                     /translation="MPALIGFVLAVAITPVAAAVARRTGLVDRPVDELKIHPAPVPVI
                     GGVAVVGAALAAWAWEGSVGGWVAGAAAAVLLAGLLDDVRSLPPWVRLVPQAIAGWML
                     TLGGLSIAPLGGLMGDAVLIVAAMALANAVNMVDGQDGLAGGLGAIAALGLALVLLRA
                     GLGTTSVLTLGGALGGFLVFNLPPARVYLGDGGAYAVGILLAAGAARASEGGWSGLVA
                     AGACLGVFAYELVASVLRRLVAGSPAARGDREHTYDRLAIRLGSRIASTAVMWVLGVL
                     ASLLGLAAAGLAPWVAAAIAAAALAMAASADTMLLPVAAFRRSHP"
     gene            complement(3159..4940)
                     /locus_tag="VE646_14325"
     CDS             complement(3159..4940)
                     /locus_tag="VE646_14325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZD81198.1"
                     /translation="MGAHVNRPRVAHITTVDLSLRFLLLAQLRALRDEGFEVTAISAP
                     GPWVADLEAEAIRHIPWGHATRSWDPRSDAVAFVELLRILRRERFDAVHTHNPKPGVL
                     GRVAARAARVPVVLNTVHGLWATPEDRARRRLPVLAAEWAAARFSDLELYQSREDLAW
                     ARRRRVVGPGRSILLGNGIDLDRFHPSLRGGERRTELRAELGFGPGDVVIGTVGRMVR
                     EKGYRELFAAAQALRAQDPKARFLVVGEADPAKPDAVTAEEVLAARPHVTFTGWREDV
                     PDLMAAMDVFVLPSWREGMPRSAIEASAAGLPLVLTDIRGCREVVSHGIEGLLVPVRR
                     PDALADALLRLVRRPDLRERMGLAARVRAERDFDERRVASTVVAVTGALLAAAGRRRL
                     PAPGGVRVVPARPHDAPALARLHSEAMPQAFLPTLGPGFMRRLYRSMVRDPEAVVLVA
                     CDDDRVVGFAAAVPSVRRFYRWFALHGAAGALAAAAPRLLRAEARRGARETSRYPAGV
                     LSLPEAELLSIAVDPAYRSRRIGRDLSDAVIDALSRRGVREVKVVVGSDNDGANRFYR
                     GLGFTRAAETQVHRGVSSNVMVVACPR"
     gene            complement(4925..>5671)
                     /locus_tag="VE646_14330"
     CDS             complement(4925..>5671)
                     /locus_tag="VE646_14330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZD81199.1"
                     /translation="KAALTPLGWPARRRPGDLIVLLYHRVGEGGAEIELPAGLFRDHL
                     VHLAERDTTLTLDQALEGRGGVVVTFDDGTADFHHTVLPLLVEHRVPATLYLATGGVA
                     NGRAGVAGGLTWGQLAEAVGTGLVTIGSHTHSHVDLSSASETHAEEEMRRSQGMIEDH
                     LGVSCRHFAYPWAVGSVAADRAARRLFDSAAWDAWRTNRAGRLDPYRLGRVPVLRSDG
                     RFFFRRKVRGQLDAEAWLYRALGRGPWGRT"
BASE COUNT          875 a         2192 c         1915 g          689 t
ORIGIN      
        1 gccacgtact cctccgtgct ttcaaccgcc ctccgacaga aatcggtgat gagccagtcc
       61 agcatggatc cagggctcga tacccgctgg atgctcgaac cgtcgaggaa gagctcgtac
      121 cggaagcgcg acgtgtcctg gatcgacccc ggactcagtc ggcgagtcag cgtgtaggtg
      181 gggacctgtt ccggctcgtc gcgatccagg aacggcgcga gaagcctgct caggacccct
      241 tgggctccgt ccagatcgct ccgtacacgg aaggtgtacc cgagcgcccg gaaccaccga
      301 tcgaatggcc agttccgatc gcgtaagaca tcgaggtggg cgagctccaa cagaggcccc
      361 ctgtctggag agacgacgat acccggggcc aaccccgggt atcgtcggtc aagcttgccg
      421 ctcaggttac gagcaggatg ctccgtgggt tccaccacag ctggagagct gcgtccccac
      481 acacgtggtg cacgaggtac cggaacagta ttgggagctg gtgtacggcc ccccggaggc
      541 gcctccacta ctggagtagg cgccgctgtg cgtgcaccag tcgctgcagt cttgggcact
      601 tgcctgaccg ggtgccgtga agaagtccgt cacgccgatc tctttcgacg gacttgagag
      661 gctgcccgag tagcagtagc aggccgagca ctgagccgac cccggaccgc cgtcctgagc
      721 ccaggccttc gttccgaagc tctggatcgc cggagccgcc cagatgagcg tgccgcccac
      781 gatggcgccg cggcgaagga gatcgcgccg gctcatccca agctcgctgt tcaggttcac
      841 tctcttcttg ccgttctcct gctcaggcat gacgatctcc ttcggctccg gccgaccatc
      901 cccacgctac cggtgtgggc cgtgcaggcg gagcgcaatc cagcgtactt ttcgtgcgta
      961 ggcaacgggc gacttctctc cccctaccga gctcccgccc tggtcaggac ctcggcgacc
     1021 ctctccacgg cttccatagc catgccctcc cacaggggca ggcacagggc tcgggcggaa
     1081 gcctcgtccg tcacgggcag accgccgttg gtgctgctcc gggcgtaggc cctggtccgg
     1141 tgcacgggcg gcgcgtagta gcggcgggtc tcgatcccct cggcagccag cgccagactc
     1201 aaggcgtcgg cgtccatgcc gaacgcttcg ggatccacga gcacggcgag atccttgtag
     1261 gtgctccgat cgcccccggg cacggatggg aaccggatgc ccggcacgcc gtccagggcg
     1321 tcccggtacc agccggcgat ccggttccgc tccccgatcc gcccttccag gtcggcgaac
     1381 gaggccaggg ccacggccgc gtggagctcg gacatccgcc cgttcagacc gacgaaccgg
     1441 cagtcgtagt cgcccgggtt cccgtagtcc cggcccatcc ggcaccgctc ggccaggacg
     1501 tcgtcgtcgg tcgcgacgat cccgccctcg gcggccacca ggggcttggt gggcgacagg
     1561 ctgaacacct cggcaccgcc gaacccgccc acgtggcggc ccgcacgcag ggacccgaac
     1621 gcgtgggcgg cgtcgaagaa caggcggatc ccatggcgat cggccaccgc ctggagcccc
     1681 tcgacgtcgc acgggctccc gtagacgtgg gtggccagca cggccgatgt ccgcaccccc
     1741 accgccttct ccaccgagcg agggtccagc gtgagcgagg acgggtcgat gtcggcgaac
     1801 accggacgga ggccgttcca ggccacggcg tgggccgtgg ccatgaacgt gaaggagggg
     1861 acgatcacat cccccgacag cccggccgcc cggagcatga gcatcagccc gctcgtgcag
     1921 gaggacaccg cgatgcagtg gtgaaccccc aggtactcgg ccgcccgcgc ctccaggcgc
     1981 cggacgaagg gcccgttcgt gagcacgccg ctccgcaaga tcgcctcgac gtcggcgccc
     2041 accacagcgg gctcggccac cctgggacgg gccagcggca gcccttcggg gaaggccggg
     2101 gccttcggcg gtcccagcgg gtgcgcgatg ggttcggtca tgcggatctc ccttcggacc
     2161 gaggttcggg cgccccgccg ctcggtgggg acggtgcggc cgcccttacg gatgtgagcg
     2221 gcggaacgcc gcgaccggca gcagcatggt gtccgcggat gcggccatcg ccagtgccgc
     2281 ggccgcgatg gccgcggcga cccacggcgc caggcccgcg gcggcgagac ccagcagcga
     2341 ggccaggacc cccagcaccc acatcaccgc cgtggaggcg atcctggagc ccagcctgat
     2401 ggccaggcgg tcgtacgtgt gttcgcgatc gccccgggcc gccggcgagc cggccaccag
     2461 gcgacggagc accgacgcca ccagctcgta ggcgaacacg cccaggcacg cgccggcggc
     2521 gaccagaccg gaccagccgc cttccgaggc ccgggcggcg cccgccgcca gcagtatccc
     2581 cacggcgtac gcgcccccgt cccccagata cacgcgggcc gggggcaggt tgaagaccag
     2641 gaaccccccg agcgctccgc ccagggtcag cacggatgtc gtcccgagcc ccgcgcgaag
     2701 cagcaccagc gccaggccga gggccgcgat cgctcccagc cccccggcca ggccgtcctg
     2761 cccgtcgacc atattcacgg cgttggccag ggccatcgcc gccacgatca agaccgcgtc
     2821 gcccatcagg cctccgagcg gggcgatgct cagcccgccg agcgtcagca tccagccggc
     2881 gatggcctgg gggaccaggc ggacccacgg agggagcgac cgcacgtcgt ccagcaggcc
     2941 ggccagcagg accgccgccg ccgccccggc cacccaccct cccaccgagc cctcccatgc
     3001 ccaggccgcc agcgcggccc cgaccacggc cacgccaccg atcaccggga ccggggcggg
     3061 atggatcttc agctcgtcca cggggcggtc caccagcccc gtcctgcggg cgacggcggc
     3121 ggctacgggc gtgatcgcca ccgccaggac gaacccgatc aacgcgggca tgccaccacc
     3181 atcacattgc tggagacgcc ccggtggacc tgggtctcgg ccgcccgggt gaagccgaga
     3241 ccccggtaga agcggttggc cccgtcgttg tccgagccga ccaccacctt cacctcgcgc
     3301 acaccccggc gcgagagcgc atcgatcacg gcgtcgctca ggtccctgcc gatccgccgg
     3361 gagcggtacg cgggatcgac cgcgatggac agcaactcgg cctccggtag ggacaggacg
     3421 ccggccgggt acctggacgt ctccctggcc ccccgtcggg cctccgcccg cagcagcctg
     3481 ggggccgctg ccgccagcgc tcccgccgcg ccgtgcagtg cgaaccaccg gtagaagcgc
     3541 cgcaccgagg gcaccgcggc agcgaacccg accacccgat cgtcgtcgca ggccaccagc
     3601 accaccgcct ccgggtcgcg caccatcgac cggtacaggc gacgcatgaa ccccggacct
     3661 agggtgggca ggaacgcctg gggcatggcc tccgaatgca gccgggccag ggccggcgca
     3721 tcgtggggcc gggccggcac cacccgcacc cccccgggcg ccggcagccg gcgtcgtccg
     3781 gcggccgcca gcagcgcacc ggtgacggcc accacggtgg aagccacccg gcgctcgtcg
     3841 aagtcccgct cggccctgac gcgggcggcc agccccatcc gctcccgcag gtccggtcgg
     3901 cggacgagcc gcaggagcgc gtcggccaga gcgtccgggc gccggaccgg gaccaggagc
     3961 ccctcgatgc cgtggctcac cacctcgcgg cacccgcgga tgtcggtcag gacgagaggc
     4021 agcccggcag ctgaagcctc gatggccgat cgaggcatcc cctcccgcca cgagggcagc
     4081 acgaagacgt ccatggccgc catcaggtcg gggacgtcct cgcgccaccc cgtgaacgtc
     4141 acgtggggac gggcggccag gacttcctcg gcggtcacgg cgtccggctt ggccggatcg
     4201 gcctccccca ccaccaggaa cctggccttt ggatcctggg cccggagggc ctgggccgcg
     4261 gcgaacagct cccgatagcc cttctcccgc accatccgcc cgaccgtgcc gatcaccacg
     4321 tcgccgggcc cgaagccgag ctcggcacga agctccgtcc ttcgctcgcc gccacggagc
     4381 gagggatgga accggtccag atcgatcccg ttgcccagca ggatcgaccg accgggaccg
     4441 accacccgcc gccgccgggc ccaggccagg tcctcccggg actggtacag ctccaggtcg
     4501 ctgaacctgg ccgccgccca ctcggccgcc aggaccggca gccgccgcct ggcccggtcc
     4561 tccggcgtgg cccacagccc gtgcacggtg ttcaggacca ccgggacccg ggccgcgcgg
     4621 gcggccaccc gccccagcac ccccggcttc gggttgtggg tgtgcacggc gtcgaaccgc
     4681 tcgcgccgga ggatccggag cagctcgacg aaggccaccg cgtccgagcg gggatcccag
     4741 gagcgggtgg cgtgccccca cgggatgtgc cggatcgcct cggcctccag atcggcgacc
     4801 cagggtcccg gcgcgctgat cgcggtcacc tcgaagccct cgtcccgcag cgcccggagc
     4861 tgggccagca gcaggaaccg caggctgagg tccaccgtgg tgatgtgcgc cacccggggc
     4921 cggttcacgt gcgcccccac ggaccccgtc ccagggctcg gtacagccac gcctcggcgt
     4981 cgagctgtcc ccgcaccttg cggcggaaga agaaccggcc gtcgctccgg agcaccggca
     5041 cccgccccag ccggtaaggg tccaggcgcc cggcccggtt ggtccgccag gcgtcccagg
     5101 ccgccgagtc gaacaggcga cgggccgcac gatcggctgc gaccgagccc accgcccagg
     5161 gatacgcgaa gtgccgacac gacaccccca ggtggtcctc gatcatcccc tgcgacctgc
     5221 gcatctcctc ctcggcgtgc gtttcgctcg cgctcgagag atccacgtgg gaatgggtgt
     5281 gcgagccgat ggtcaccaga cccgtcccga ccgcctcggc cagctgcccc cacgtgagcc
     5341 cgccggcaac ccccgccctc ccgttcgcca cgccaccggt ggccaggtac agggtggccg
     5401 gaacgcggtg ctcgaccagc agcggcagga ccgtgtggtg gaaatcggcg gtcccgtcgt
     5461 cgaacgtcac caccacacca ccccgacctt ccagggcctg atccagcgtg agcgtggtgt
     5521 cgcgctcggc caggtggacc aggtgatcgc ggaacagccc ggccgggagc tcgatctcgg
     5581 cgccgccctc tcccacccgg tggtacagga ggacgatcag gtcaccgggg cgccgacgcg
     5641 ccggccaccc cagcggggta agcgccgcct t
//
LOCUS       DASQQQ010000382         2530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_245012, whole genome shotgun sequence.
ACCESSION   DASQQQ010000382 DASQQQ010000000
VERSION     DASQQQ010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245012"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VE646_14335"
     CDS             <1..690
                     /locus_tag="VE646_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81200.1"
                     /translation="VFEAGGPDELYVAGEPVPFMSQVRDTGRAVIYRLDHTGRVVDTL
                     LTYPGSSWTPIQLPNGRTSFAKPRFSAEPRLDARPGIAAASYIPTYVIDVRSPDGRPT
                     LRIARQYENAPVVKAVRDSVLDRLASGPNRIPREALASIPFESQIPAIEGLTMDDRRR
                     VWIDPYAPDQPARRDLFDTEGRFQGPAYLPEPIRLKDIRGGRACGAVHDPDDRSVQVV
                     CYRVMEPPPKA"
     gene            833..>2530
                     /locus_tag="VE646_14340"
     CDS             833..>2530
                     /locus_tag="VE646_14340"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZD81201.1"
                     /translation="MALPDLSIRRPVATAMACLAAVLLGVIAFTRLPVNLLPDVALPT
                     LAIWTEDPEAGPAEVERFVTEPIENAVARVPSVKAVSSTSREGGSVVRLQFVWGTDMD
                     YATLNVREKLDNVGYDLPERAGRPTILRSDPTSDPILTLAAVSDSAGLWELKELAENV
                     FKRRLEQIDGIAQAVVAGGLEREIHVAVDPARLEALGVTLDDVSSALAAANVSAPGGT
                     IRRGRYRYALRALGEFQTVDEIGDTPVDRGAGRGTDGSAGGAQVRLRDVAAIQDAFAD
                     RETIARYDGRESVGLLLYKEAGANTVRAASRVRDVLEQLRQEYPAVELTVASSQAGFI
                     RDAISNVVQALALGAVLAFLVLFLFLHDARYPVAISLSIPISVVLTFGLLYAFGVSLN
                     IMSLGGLALGVGMLVDNSIVVLENIFRHREQGLGMREAATRGAREVQGAITASTLTTI
                     AVFGPIIYVKGVAGELFGDLSIAVATSLLISLAVALTLLPMMASRMGAGAGADDDIEG
                     GGVARDESGRRSGLRDVFRGAPVWRWPAALARWLVRLATRSIRTTLSFLGALLRFWGG
                     "
BASE COUNT          321 a          876 c          973 g          360 t
ORIGIN      
        1 gtcttcgagg cgggcggccc ggacgagctg tacgtggcgg gcgagccggt ccccttcatg
       61 agccaggtgc gggacacggg ccgcgcggtg atctatcggc tggaccacac cggccgggtc
      121 gtggacacgc tgctcaccta cccgggctcc agctggacgc cgatccagct gcccaacggg
      181 cgcacgtcgt tcgcgaagcc ccgcttcagc gccgaaccgc ggctggacgc gcggccgggg
      241 atcgcggccg cgtcgtacat cccgacctac gtcatcgacg tccggtcgcc cgatggccgg
      301 ccgaccctgc ggatcgcccg gcagtacgag aacgccccgg tcgtgaaggc ggtccgggac
      361 agcgtgctgg accggctggc ttcgggaccg aaccggatcc cccgggaggc gctggcgtcg
      421 atcccgttcg agtcccagat cccggcgatc gagggtctga cgatggacga ccgccggcgg
      481 gtgtggatcg acccctacgc ccccgaccag ccggcgcgcc gggacctgtt cgacaccgag
      541 ggccgcttcc aggggccggc ctacctgccc gagcccatcc gcctgaagga catccggggt
      601 ggccgcgcat gcggtgccgt gcacgatccc gacgaccgct ccgttcaggt ggtctgctac
      661 cgggtcatgg agcctccgcc gaaggcctga gcgccggctc cgctccacgg ccatgccgga
      721 tccccgctcg ccgccggccc gccggccggc gcggcgctcg aaggcgcacc ggcatcgctc
      781 cgggcgctga gcccacccgg cggagcgccc ggccgccggc ccgagcctga cgatggctct
      841 ccccgacctg tcgatccgcc ggcccgtcgc caccgcgatg gcctgcctgg ccgcggtgct
      901 gctgggcgtc atcgcgttca cgcgcctgcc ggtgaacctg ctacccgatg tggccctccc
      961 gacccttgcg atctggaccg aggacccgga ggcgggcccg gcggaggtcg agcggttcgt
     1021 caccgagccg atcgagaacg cagtggcccg ggtgccgagc gtgaaggcgg tgagctcgac
     1081 cagccgggag ggcggctcgg tcgtccggct ccagttcgtc tggggcacgg acatggacta
     1141 cgcgacgctc aacgtgcggg agaagctgga caacgtgggt tacgatctgc cggagcgggc
     1201 gggccggccg acgatcctgc gctcggaccc gaccagcgat ccgatcctga cgctggcggc
     1261 cgtctccgac tccgccggtc tgtgggagct gaaggagctg gcggagaacg tcttcaagcg
     1321 gcggctggag cagatcgacg ggatcgccca ggcggtggtg gcgggcggcc tggagcggga
     1381 gatccacgtg gcggtggacc cggcccggct ggaggcgctg ggcgtgacgc tggacgacgt
     1441 gtccagcgcg ctggcggcgg cgaacgtgag cgcgccgggc ggcaccatcc ggcgcggccg
     1501 ctaccggtac gcgctccggg cgctgggcga gttccagacg gtggacgaga tcggtgacac
     1561 gccggtggat cggggggccg gcaggggcac ggacgggagc gccggcggcg cgcaggtccg
     1621 gttgcgggac gtggcggcga tccaggacgc cttcgcggac cgggagacga tcgcccgcta
     1681 cgacggccgg gaatcggtgg gcctcctcct atacaaggag gcgggtgcca acacggtccg
     1741 ggcggcgtcc cgggtccggg acgtgctgga acagctgcgc caggagtacc cggccgtgga
     1801 gctgacggtc gccagctcgc aggccgggtt catccgggac gcgatctcga acgtggtgca
     1861 ggcgctcgcc ctgggcgcgg tcctcgcctt cctggtgctc ttcctgttcc tgcacgacgc
     1921 gcgctacccg gtggcgatct cgctgtcgat ccccatcagc gtggtgctga ccttcgggct
     1981 gctctacgcg ttcggggtca gcctgaacat catgagcctg ggcggcctgg ccctgggcgt
     2041 cgggatgctg gtggacaact cgatcgtggt gctggagaac atcttccggc accgcgagca
     2101 ggggctgggc atgcgggagg cggcgacccg gggcgcccgg gaggtgcagg gcgccatcac
     2161 cgcgtccacg ctcaccacga tcgccgtgtt cggcccgatc atctacgtga agggggtggc
     2221 gggcgagctg ttcggcgacc tgtcgatcgc ggtggcgacc tcgctgctta tctcgctggc
     2281 cgtcgcactc acgctgctgc cgatgatggc gtcccggatg ggggccgggg cgggtgccga
     2341 cgacgatatc gagggcgggg gcgtggcacg cgacgagtcc ggccgccgca gcggcctgcg
     2401 ggacgtgttc cggggagccc cggtctggag gtggccggcg gccctggccc ggtggctggt
     2461 tcgcctcgcg acgcgatcga tccggacgac gctctcgttc ctgggcgcgc tgctccggtt
     2521 ctggggcggg
//
LOCUS       DASQQQ010000383         6189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_246133, whole genome shotgun sequence.
ACCESSION   DASQQQ010000383 DASQQQ010000000
VERSION     DASQQQ010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246133"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2870)
                     /locus_tag="VE646_14345"
     CDS             complement(<1..2870)
                     /locus_tag="VE646_14345"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HZD81202.1"
                     /translation="MAPPTSFRPDPQQDLVLEHQRGALLVTGAAGVGKSATLRERLAR
                     LIERGANAERVALVVRSRRDRGETRRALLDRLGRSLPGLHVVTIQGLAHHVMGQRFGA
                     IGFREAPRVLDAADQFTMVQELLRDEREHGRDRWPVYGGLLSLSGFADQVRQLVLRAQ
                     ESLIDPEHIAERAGDAPPGWAELATFYRRYLDVLAERGSLDFAGLVWQAARAVGTGDR
                     LFDHVLVDDFQDTTIGAEALLQRLGAEDLVLAGDRGSHVFSFQGTTDLPIRRAVDRFN
                     AIEVRLEVPHRGGHVRLEAWRAAHTSEEHAAVARELRRVHVEEGVPWHGLAVLVRRQG
                     PQVASLLRALDDAGIPRTAPEARLSIASAPSTRPYLLALRWIAAPPQARDELIEPVLT
                     SELGGLSPASARSLLRLTRATGRPSRDALQIDEGLTEQERLNLERMRRVLAAAEIRAD
                     SVLDAFAVLWKDLPCSTRLVEGAERSHDARVDLDGVLQLSSAVAQAASSSDQSTEAFL
                     RQAELGEGGPTIAGRGAPEHDAVEVLTAHAASGRQFDTVVVVGAVEGDFPSLARPEPM
                     FDLPSLSGALPRSSVNRMRLEDERRLFGVAVSRARRRVLLTASDPHGSESDEAVATRF
                     AQERGIGWRPVPAADASDPVSVAEAAAAWRRMLAAAGGAAPDRLGALAGLLALRDDPG
                     RWWFQHDWSGPSRPPREELRLSYSRLDHLENCELQYLLADELGLDPGGGYQAWVGKLL
                     HQLIEDCENGAIERTPEAFVGAITSRWQPARFPSHAVSEAERLHAIRVIVPNWYQRYG
                     DLAAEATERRFRFDYEGAVVNGVIDRIGPVPEGHRRITDYKTGNADNAPKPQESLQLG
                     IYYLAVNECEDLASYRPVHGVELAYLAGKKRQGELLPLSWPVSERDEERYKTRMRERL
                     SALIGRIGALDQLGRYVPNTGANCFFCRFQTLCSRYPEGGEV"
     gene            complement(2908..4014)
                     /locus_tag="VE646_14350"
     CDS             complement(2908..4014)
                     /locus_tag="VE646_14350"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZD81203.1"
                     /translation="MFGRSWRIGSVRGIPVNVDSSWVWIAILITYTLWAMFDGRFASV
                     GPVEAFGLAVFVAALFFGSVFLHELAHAVVARLNEIPVYGITLVIFGGFTSAKSDEKG
                     PGPSFLISAVGPGMSGILGAAFWGVSGMVGDVNHPLAVAFGYLGWVNGAMAVFNVLPG
                     LPLDGGRMLEAAVWAISRDHDRATRVAAVAGMVVGGLLVALGIYRIAAHDLELGVWFA
                     IIGAFIFQAARGSQQQISLRRRLAGATVADAMDPPPPAVPADLALTDVLERYLRGHEG
                     EAFPVVESPSRVIGLITMDSARRIGRYDPFRPARDGMIPLSQVVTTEMGEPLDRAAAR
                     LGAGRAALVLRDGQLVGSISGGGVARWMTQVRPA"
     gene            complement(4279..5181)
                     /locus_tag="VE646_14355"
     CDS             complement(4279..5181)
                     /locus_tag="VE646_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013286050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZD81204.1"
                     /translation="MGRTLVGISSWTERTLVQKSDFYPSSARSAEARLKYYAAQFPIA
                     EVDSTYYYPPSERNSVLWIERTPKDFTFNIKAYSLLTNHPTRRDSLYKDLAAELPDEL
                     RDKRNLYRDKMPQEIVDELWQRFRDALMPLHSAGKLGAVLFQFPQWFVIARGNKAYIE
                     ECKERLADFRLAIEFRHKSWMEERNREETLSFLSEHDLPFVCVDMPQGFDSSLPPIAA
                     VTTDDLAVVRFHGRDPKAWTTKAETAAERFRYEYSEGELEEWVPRIEQLSQQARETHV
                     IMNNCYRDFAVNNARQLGSLLDLD"
     gene            complement(5192..5524)
                     /locus_tag="VE646_14360"
     CDS             complement(5192..5524)
                     /locus_tag="VE646_14360"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HZD81205.1"
                     /translation="MNPAAKQVARWIWFPFAVVLRFLLRNGKRIAVTIGGMMLLVAGM
                     IMMVTPGPGLVVIIAGLAVLATEYVWAQRALNLAKRKAQQAKDKVTGRSRRGTASSTS
                     QPDRSGSD"
     gene            complement(5617..5997)
                     /locus_tag="VE646_14365"
     CDS             complement(5617..5997)
                     /locus_tag="VE646_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81206.1"
                     /translation="MRRVIAVIAYGLAGMLVTGVLTAGAWALAGKDISQPTPPTPPLA
                     VAHPSPKPTEPISSGREDRSQEVASPSGSSDEHGGSLDQGRSGASATPSQDRSGTPDG
                     VGSIGVSDSSADGHTGSYDEGGDD"
     gene            complement(6037..6135)
                     /locus_tag="VE646_14370"
     CDS             complement(6037..6135)
                     /locus_tag="VE646_14370"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81207.1"
                     /translation="MRELAERWGGEVMVQAPTDGGTRIEVRLRATS"
BASE COUNT          937 a         2204 c         2137 g          911 t
ORIGIN      
        1 aagacctccc cgccctccgg gtaccgagaa cagagcgtct ggaacctgca gaagaagcag
       61 ttcgcccccg tgttcggcac gtaccgcccc agctggtcca gcgcgccgat ccggccgatc
      121 agtgcggaga gccgttcgcg catccgcgtc ttgtagcgct cctcgtctcg ctccgagacc
      181 ggccaggaca gcggcaggag ctctccctga cgcttcttcc ccgccaggta ggcgagctcg
      241 acgccgtgca ccggacggta cgaagccagg tcctcgcact cgttcacggc caggtaatag
      301 atgccgagct gcaggctctc ctgtggtttg ggggcgttgt cggcgttccc cgtcttgtag
      361 tcggtgatac ggcggtgccc ctcggggacg ggtccgatcc ggtcgatgac gccgttcacc
      421 acggcccctt cgtagtcgaa gcggaaccgg cgctcggtgg cctcggctgc gaggtccccg
      481 tagcgctggt accaattcgg cacgatcacc cggatcgcgt ggaggcgctc ggcctcggac
      541 acggcgtgcg aggggaaccg agccggttgc cacctcgacg tgatggcccc cacgaacgcc
      601 tcaggggtcc gctcgatcgc accgttctcg cagtcctcga tgagctgatg caggagcttg
      661 cccacccagg cctggtagcc cccgccgggg tccaggccca gctcgtcggc caggaggtac
      721 tggagctcgc agttctcgag gtgatccagg cgcgagtacg acagacgcag ctcctcccgg
      781 ggcgggcgcg acgggccgct ccagtcgtgt tggaaccacc agcgcccggg gtcgtcccgc
      841 agcgccagca gccccgccag tgcgcccagc cggtccgggg cagctccgcc cgcggcggcg
      901 agcattcggc gccaggcggc cgcggcctcc gccaccgaga ccggatcgct cgcgtcggcg
      961 gctgggaccg gccgccaccc gatcccccgt tcctgggcga atcgtgtggc caccgcctcg
     1021 tcggactcgg agccgtgcgg atcgctcgcc gtgagcagca cccggcgtcg ggcccggctc
     1081 acggccacgc cgaagagccg ccgctcatcc tccagccgca tccgattcac gctggagcgg
     1141 ggcagggccc cggacagcga gggcaggtcg aacatgggct cgggtcgggc gaggctgggg
     1201 aagtcgcctt ccaccgcccc caccacgacc accgtgtcga actggcgtcc ggaggctgcg
     1261 tgcgccgtga gcacctcgac cgcgtcgtgt tcgggtgctc cacgaccggc gatggtcggc
     1321 ccgccctcgc cgagttcggc ctgccgcagg aaggcctccg tggactgatc ggaagaggac
     1381 gcggcctggg ccacggcgct cgagagctgg agcaccccat cgaggtccac ccgcgcatcg
     1441 tgggatcgct ccgccccctc gaccagccgg gtggaacacg gcaggtcctt ccacagcacg
     1501 gcgaacgcgt ccagcacgga gtcggcacgg atctcggcgg cggcgagcac cctccgcatg
     1561 cgctccaggt tcagtcgctc ctgctcggtg aggccctcat cgatctggag ggcatcccgc
     1621 gacggccgtc cggtggccct ggtcagcctg agcagcgatc tggccgaggc gggggagagg
     1681 cccccgagct cggaggtgag gaccggctcg atcagctcgt cgcgcgcttg gggcggtgcg
     1741 gcgatccacc gcagggccag caggtagggc cgggtggagg gcgcggaggc gatcgagagc
     1801 ctggcctcgg gcgccgtgcg ggggataccc gcatcgtcca gggctcggag gaggctcgcc
     1861 acctgaggac cctgccgccg cacgagcacg gccaagccgt gccatggcac cccctcctcc
     1921 acgtgcaccc gccggagctc ccgggccacc gcggcatgtt cctcggatgt gtgggcggct
     1981 cgccaggcct cgagccggac gtggccgccg cggtgcggga cttccagcct gacctcgatg
     2041 gcgttgaacc gatcgacggc acggcggatc ggcagatcgg tcgttccctg gaaggagaac
     2101 acgtgcgatc cccggtcgcc ggccagcacg aggtcttcgg cgccgagccg ctggagcagg
     2161 gcctcggcgc cgatcgtggt gtcctggaag tcgtcgacca gcacatggtc gaagagccgg
     2221 tcgccggtgc cgaccgccct ggccgcctgc cacacgagcc ccgcgaagtc gagggacccg
     2281 cgctccgcca gcacgtccag gtaccggcgg tagaaggtcg cgagctccgc ccaccccgga
     2341 ggggcatctc cggcccgctc cgcgatgtgc tcgggatcga tcagcgattc ctgtgcacgg
     2401 aggaccagct ggcggacctg gtccgcgaag ccgctgaggg acaggagccc tccgtagacc
     2461 ggccaccgat cccgtccgtg ctcgcgttcg tcgcgcagga gctcctgaac catcgtgaac
     2521 tgatcggcgg cgtccagcac ccgaggtgcc tcccggaagc cgatggcgcc gaagcgctgg
     2581 cccatcacgt gatgggccag tccttggatc gtgaccacgt gcaggccggg caacgagcgg
     2641 cccaggcgat ccagcagggc ccggcgggtc tcgccgcggt cccggcgcga gcggaccacc
     2701 agggccacgc gctcggcgtt cgccccgcgc tcgatgaggc gggccaggcg ctcccgcagc
     2761 gtcgccgact tgcccacgcc ggcggcgccg gtgaccagga gggcgccgcg ctggtgctcg
     2821 agcaccaggt cctgctgagg atcgggccgg aaggacgttg ggggagccac gccttcggat
     2881 gctaccgccg ggggccgaca gccccggtca ggcgggacgg acctgggtca tccaccgggc
     2941 cacgccgcca ccggagatcg atcccaccag ctgcccgtcg cggaggacca gcgccgctcg
     3001 tcccgcgccc aggcgcgccg cggcccggtc gagcggttcc cccatctcgg tggtgactac
     3061 ctgcgagagc gggatcatgc cgtcgcgcgc cggacggaac gggtcgtatc ggccgatccg
     3121 gcgtgccgag tccatcgtga tcagaccgat cacgcggctc gggctctcga cgacggggaa
     3181 ggcctcgccc tcgtggcccc ggaggtagcg ctccagcacg tctgtcaggg ccagatcggc
     3241 cgggacggct ggaggcggcg ggtccatcgc gtcggccacc gtggctccag ccagccgccg
     3301 ccgcagggag atctgctgct gggaccctcg ggcggcctgg aagatgaacg cgccgatgat
     3361 cgcgaaccaa acgccgagct cgagatcgtg cgccgcgatc cggtagatgc ccagggccac
     3421 caagagcccc cccaccacca tcccggccac ggcggccacc cgggtggccc ggtcatggtc
     3481 gcgggagatc gcccagaccg ccgcctcgag catccgtcct ccatcgaggg gcagcccggg
     3541 aagcacgttg aacacggcca tcgcgccgtt gacccacccc aggtagccga aggcgacggc
     3601 cagcgggtgg ttcacgtcgc cgaccatccc cgacaccccc cagaaggccg cacccaggat
     3661 cccgctcatc ccgggcccca ccgcggagat gaggaacgat ggacctgggc ccttctcgtc
     3721 ggacttggcg ctggtgaacc cgccgaagat caccagggtg atcccgtaca cggggatctc
     3781 gttcaggcga gccaccaccg cgtgcgcgag ctcgtggagg aagaccgacc cgaagaacag
     3841 ggctgccacg aagaccgcga gcccgaaggc ctccaccggt ccgacgctcg cgaatcgccc
     3901 gtcgaacatg gcccacagcg tgtaggtgat caggatggcg atccacaccc aggaggaatc
     3961 gacgttgacc gggatgcccc ggaccgaccc gatccgccac gacctgccga acaccctcgc
     4021 tcctctcttg gggaaccgag agtactccag ccgacgcgca acggacctcc gatggaccgg
     4081 ggcccgaagg tgaccggcgg atcggttccc cgcccctgat gcgagcctcc gctgttaggc
     4141 tgggaagggt tccggagccg gcatcacggt tccgggtgga tagtctggcc cgggctaccc
     4201 tcccttccac gcgggcgtgc ccgcgggtcc gggccagact cctttccctg cgtctgctcc
     4261 ccaggcgctg gacgacgatc agtcgagatc gagcagggaa ccgagctgtc gcgcgttgtt
     4321 gacggcgaaa tcccggtagc agttgttcat gatcacgtgg gtctcgcgtg cctgctggga
     4381 cagctgctcg atgcgaggga cccactcctc caactcgccc tccgagtact cgtatcggaa
     4441 gcgttcggcg gccgtctcgg ccttggtcgt ccatgccttc gggtcgcgtc cgtggaaccg
     4501 gaccacggcc aggtcgtcgg tcgtcaccgc cgcgatcggc ggcaggctcg agtcgaagcc
     4561 ttgcggcatg tccacgcaga cgaacgggag gtcgtgctcg ctcaggaagg agagggtctc
     4621 ctcgcggttg cgctcctcca tccagctctt gtgccggaac tcgatggcca gacggaagtc
     4681 cgccagccgc tccttgcact cctcgatgta ggccttgttc ccacgggcga tcacgaacca
     4741 ctgggggaac tggaacagca cggctccgag cttgcccgcc gagtgcaggg gcatcagtgc
     4801 gtcccggaac cgctgccaga gctcgtcgac gatctcctgc ggcatcttgt cgcggtacag
     4861 gttccgcttg tcccgcagct cgtccggcag ctcggccgcc aggtccttgt agagcgaatc
     4921 ccgtcgggtg gggtggttcg tgaggaggga gtaggccttg atgttgaagg tgaagtcctt
     4981 gggggtgcgt tcgatccaca gcaccgagtt tcgctctgag ggcgggtagt agtaggtcga
     5041 gtccacctca gcgatgggga actgcgccgc gtagtacttc aggcgtgctt ctgcggacct
     5101 ggccgaggac gggtagaaat cgctcttctg caccagggtg cgctcagtcc agctcgagat
     5161 gccgaccagg gtccgcccca cgcgttccag gttagtcgga acccgatcga tcgggttggg
     5221 aggtgctgga cgccgtgcct cgacggctcc gcccggtgac cttgtccttc gcctgctggg
     5281 ctttgcgctt ggcgaggttc agggcccgtt gagcccaaac gtactcggtg gccagcacgg
     5341 cgagtcccgc gatgatcacg accagtcctg gtccaggcgt gaccatcatg atcatgcccg
     5401 ccaccagcaa catcatcccg ccgatcgtga cggcgatccg cttcccgttg cgcagcagga
     5461 agcggaggac caccgcgaag gggaaccaga tccaccgcgc tacctgcttc gccgccggat
     5521 tcatcgcatc accgcccaca ccttaccgtt cgcggatggg cgagattggc gccggagctt
     5581 ccgtgtcgat tggaagtcga gcggtcagcg cggaggtcag tcgtcgccgc cctcgtcgta
     5641 ggaaccggtg tggccgtcgg ccgacgagtc ggagacgccg atggagccga ccccatccgg
     5701 ggtgccggag cggtcctgcg agggcgtggc cgatgcgccg ctcctgccct ggtccaggct
     5761 cccgccatgc tcgtcggacg aacccgaggg cgatgccact tcctgcgaac gatcctcgcg
     5821 tccgctcgag atgggttcgg tgggcttggg ggaaggatgc gccaccgcca gcggcggggt
     5881 cggcggggtc ggttggctga tgtcctttcc ggcgagcgcc caggcgccgg cggtgaggac
     5941 gcccgtcacg agcatcccgg ccagcccgta cgcgatgact gcgatgaccc gtcgcatgtt
     6001 ccaggtcagc ctatccaccg gtcggataag agccggttaa gaggtggccc gcagccggac
     6061 ctcgatccgg gtgcccccgt cggtgggcgc ctggaccatc acctccccgc cccacctctc
     6121 ggccagttcc cgcacgatgg ccaggcccag gcctgaaccg ccggccgggg ccgcacggcc
     6181 ccggaagaa
//
LOCUS       DASQQQ010000384         5189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_246373, whole genome shotgun sequence.
ACCESSION   DASQQQ010000384 DASQQQ010000000
VERSION     DASQQQ010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246373"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VE646_14375"
     CDS             complement(<1..793)
                     /locus_tag="VE646_14375"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HZD81208.1"
                     /translation="MTARMGAYRDIVDAIGNTPLVGMPRMSPSPAVRLWAKLEGHNPT
                     GSTKDRIAKKMIEAAEASGELTAERTILEPTSGNTGIALAMVARRRGYRLTVVIPDNA
                     SEERIRLLELFGAEIVYSPGDKGTNGSIEVARALARDDRYLMLFQYGNPANPQAHYEG
                     TAGEIVRDLPRITHFVAGLGTGGTLTGCGRRLHEHDPGIKVIAAEPELGDLVYGLRSL
                     EEGFVPPIFDEGVLDRKFLVNSSDSLRATRELTEREGIFAGISSGA"
     gene            complement(843..1271)
                     /locus_tag="VE646_14380"
     CDS             complement(843..1271)
                     /locus_tag="VE646_14380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZD81209.1"
                     /translation="MGHPTVQVRPLGPEDEDLLDAALQLSRGEPSPAPDLFLGDPRTH
                     AFVALEGRDVVGWAFGQELLRPEGRWTILLYEIGTTEEARRRRVGHELLEAFAELARS
                     RGHQRMWLYADAGAKAARALYPEAGGERAPGETGYWWVFA"
     gene            complement(1280..1555)
                     /locus_tag="VE646_14385"
     CDS             complement(1280..1555)
                     /locus_tag="VE646_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HZD81210.1"
                     /translation="MSVKVKLPTILRKHAGGEPQVQAEGETLRDLLSDLESRYPGITK
                     NIVSDEGGLHRFINIYVNDEDVRYLGSLETSVKEGDTVSILPAVAGG"
     gene            complement(1651..2451)
                     /gene="moeB"
                     /locus_tag="VE646_14390"
                     /pseudo
     CDS             complement(1651..2451)
                     /gene="moeB"
                     /locus_tag="VE646_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997855.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
     gene            complement(2448..2849)
                     /locus_tag="VE646_14395"
     CDS             complement(2448..2849)
                     /locus_tag="VE646_14395"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HZD81211.1"
                     /translation="MGIELDGVFFKEIVEQGLREFPNECCGLVAGKDGSPVKVFAMRN
                     LDASPVSYRLDPKEQLQVFELLDREGWELWGIYHSHTHSEAYPSETDRRLAFYPDARY
                     LVLSLQDRERPELRAFRIGEDQISEEELSIT"
     gene            complement(2880..3869)
                     /gene="selD"
                     /locus_tag="VE646_14400"
                     /pseudo
     CDS             complement(2880..3869)
                     /gene="selD"
                     /locus_tag="VE646_14400"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
     gene            4525..>5189
                     /locus_tag="VE646_14405"
     CDS             4525..>5189
                     /locus_tag="VE646_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81212.1"
                     /translation="MKNKKGLLAALGAFTLLMALTLPASAGSVGNASGFEDDDGNLAV
                     DSTFDWNSFAPTTWEGTAPYRQSDKVLSGWTFKGIEDAQATNADSAFAGGTKQDDNCP
                     TVNTGKAPNKDDLKRIYLSTKVVGGHTYLNLAWVRIPQNTTSPSAHIGFEFNKGTDGT
                     CGGSSNGLVKRVAGDMLIVYDFEGGATDTPVITLRRWVTSGACEVGSDSPPCWGPATN
                     LTDA"
BASE COUNT          809 a         1864 c         1739 g          777 t
ORIGIN      
        1 ccgcgcccga ggagatcccg gcgaagatcc cctcgcgttc ggtcagctct cgggtggcgc
       61 gcagggagtc ggaggagttc accaggaact tccggtcgag cacaccctcg tcgaagatcg
      121 gcggcacgaa cccctcctcc agcgagcgga ggccgtagac cagatcgccg agctcgggct
      181 cggcggcgat caccttgatg ccgggatcgt gctcgtggag gcggcggccg catccggtga
      241 gcgtgcctcc cgtgccgagc ccggccacga aatgcgtgat ccgaggcagg tcccggacga
      301 tctcccccgc ggtcccctcg tagtgggcct gcgggttggc cgggttgccg tactggaaca
      361 gcatcaggta ccgatcgtct cgggccaggg cccgggccac ctcgatggag ccgttcgtgc
      421 ccttgtcccc cggcgagtag acgatctcgg cgccgaacag ctccaggagg cggatccgct
      481 cctcgctggc gttgtcgggg atcacgaccg tgagccggta ccccctgcgc cgggccacca
      541 tggccagggc gatgccggtg ttgcccgacg tgggctccag gatcgtgcgc tcggcggtga
      601 gctcgcccga ggcctcggcg gcctcgatca tcttcttggc gatccggtcc ttcgtcgatc
      661 cggtcgggtt gtggccctcg agcttggccc acaaccggac cgcggggctg ggcgacatgc
      721 ggggcatacc gaccagcggc gtgttcccga tggcgtcgac gatgtcgcgg taggcgccca
      781 tgcgagcggt catggcagtg gggccgccga ggaccggaag ggacgcaccg ctcgcgcccg
      841 cctcacgcga agacccacca gtagcccgtt tcgccggggg cccgctcccc gcccgcctcc
      901 ggatagagcg cacgcgccgc cttcgccccc gcgtccgcgt acagccacat ccgctggtgc
      961 ccccgagacc tggccagctc ggcgaaggcc tcgagcagct cgtgcccgac ccgtcgccgc
     1021 ctggcctcct cggtggtccc gatctcgtac aggaggatcg tccaccggcc ctcgggacgg
     1081 agcagctcct ggccgaaggc ccatcccacc acgtcgcgcc cttccagcgc gacgaacgcg
     1141 tgcgtgcgcg ggtcccccag gaacaggtcg ggtgccggcg acggttcgcc ccgcgacagc
     1201 tggagggcgg cgtccagcag gtcctcgtcc tccgggccga gcggccgcac ctggaccgtc
     1261 gggtggccca tcgagatcct cagccgccgg cgacggcggg caggatcgag acggtgtccc
     1321 cctccttcac ggaggtctcc agcgagccga ggtagcgcac gtcctcgtcg ttcacgtaga
     1381 tgttgatgaa ccggtgcagc ccgccctcgt cggagacgat gttcttcgtg atccccgggt
     1441 accgggactc gaggtccgac agcaggtccc gcagggtctc accctcggcc tgtacctggg
     1501 gctcgccccc ggcgtgcttg cgaaggatgg tgggcagctt caccttcacg gacatcgcgc
     1561 tcgtcatgct ccttccccgc tcgtggcccc gctcgtggcc ccgctcgtgg acaccccgtg
     1621 ctcggcctcg ggcggctcct ggccgggggt caggccgcag aaggcctcgt agtcgatcag
     1681 ctcggtgatg gtgggctcct tgccgcacac cgggcagtcc gcatcccacc ggacgttgac
     1741 cgtgctgaac tccatggcca gcgcgtcgaa gatcagcagc cggccgatca gggggtcgcc
     1801 cagttcggtg atcagcttga tggcctcggt ggcctggatg cagccgacga tgcccggcag
     1861 cacgccgaag acgccgccct cggcgcacga gggcacggtg cccggcgggg gcggctgcgg
     1921 gaacaggcac cggtagcacg gcgcgtcctg cccgggcatg aacacggtgg cctgcccgtc
     1981 gaactggtag atcgagccgt agaccagcgg cttgcccagc atctgggtgg cgtcgttcac
     2041 gaggtagcga accgggaagt tgtcggtgcc gtccacgatc acgtcccagg gctccaggat
     2101 ctcgaacgcg ttggccgagg acaggatcgt gtcgtagggc acgatgtcga tcgttgggtt
     2161 caggccccgc agggtgtcca cggcgctgtc caccttcgag cgacccacgt cggcggtggc
     2221 gtggaggatc tgccgctgca ggttcgactc gtccacccgg tcgaagtcca ggatgccgag
     2281 cgtccccacg ccggccgcgg ccaggtacat cgacaccggc gagccgagcc cccccgcgcc
     2341 gatgcacaac accttcgcgt cgagcagctt gcgctgtccc tgcccaccga tccccggaag
     2401 gatgatgtgg cgagagtagc gacggacctg ctcctcggtg aacccggtca cgtgatgctc
     2461 agctcctctt cgctgatctg gtcctccccg atccgaaacg cccgcagctc cggccgctcc
     2521 cggtcctgca gcgacagcac caggtatctg gcgtccggat agaaggcgag cctccggtcc
     2581 gtctcggagg ggtacgcctc ggaatgcgtg tgggaatggt agatgcccca caactcccat
     2641 ccttcccgat ccagcagctc gaacacctgc agctgctcct tgggatcgag ccggtaggag
     2701 acggggctcg cgtccaggtt cctcatggcg aagaccttga cgggcgatcc atccttgccc
     2761 gccaccagac cgcagcattc gttggggaac tcgcggagcc cctgctccac gatctccttg
     2821 aagaacacgc cgtccagctc gatccccatc gcacctgcaa taactctatc agcgcggtgc
     2881 tattccccga gtcgcgatcc aacccgtatc tggccgggcc gtccggcctc ggtccgaccg
     2941 atgcgcgcgg cccggacccc ccgctccacc agcgcggtct cgagcgcgga agcgtcccgc
     3001 acggcgatca ggagcccgcc ggaggtctgg gcatcggcca ggacgacctg ctcgggctcg
     3061 gtcagctctc cgggctccag gaagggccgg acgaaggcca ggttgcgctt gctgccggat
     3121 ggcacgacat cggcccgggc cagctcgagg gtgccgggca gcagggggat ctcctccgcg
     3181 tcgagcacgg cggcgacccc ggagccctcg agcagcttct gcaggtggcc caacagcccg
     3241 aacccggtga cgtccgtggc cgccgagacc ccggcctcca gcatcgcctc ggccgccccc
     3301 cggttcagct ccaccatcag ttcgacggcc gcccggatct gctcgggcgt ggcctgtccc
     3361 cgcttgatcg cggtggaggc gatgcccagc ccgagcggct tggtgaggaa caggtccgcg
     3421 cccgccggcg cggtcgagtt ccgcaccagt cggtcggccc gcgcgaaccc cagggccacc
     3481 atcccgtact tgggctcggg atcggtgatc gtgtggccgc cgaggaccac ggtcccggca
     3541 cgagcggcga cgtcctgtcc tcctcggagg acctcggtca gcagatcgag cggcaggtcg
     3601 tccaccggcc agctcaccag gttcaacgcc acggcggggg ccgcgcccat cgcgtacacg
     3661 tcgctgaagg cgttcgccgc ggcgatccga ccccagtcgt aggggtcgtc cacgatgggc
     3721 gtgaagaagt ccaccgtcgc cacgagcgcc cgcccgtcgg gcagacggta ggcggcggcg
     3781 tcgtcgccgg tctccgccga aaccagcacc tcctccggca tcgtcggagc ctcgagtcgg
     3841 ctcaggacct gagccaggtc accgggaccg agcttgcacg ctcagccggc gccgtggctg
     3901 aagctggtca gccgcacctc gtcccgcatg agccgaggat acgcggggcc ggcggtcatg
     3961 cccgaaccgc cacgggtgtc ggccctggat cagcccgcca ggttgtcggt catcaaccgg
     4021 ctccacagcc tgacgagcga gcgggccccg ctcgcacgga cgcaggtcgg tgccgagaag
     4081 cgaaacccgg cgggtcgacg gaaccgcatg agttgacaca ccccaccccg cgggagctgg
     4141 atgcctcatt gcgccggccg atggggatcc accgtgccgg agacgctgac ggaaagagcc
     4201 ccgtcacccc cgaccttcat cggacgacct agggccaacg tccatccccg ggtacgcctc
     4261 ctaggtcatt cgtccattcc tgcttcctat ccgccaccaa tcccccgttt gggggatgcc
     4321 tcgtccaggg tgagttagga caatcggccg acagcgttag ggggataggg gggcttacga
     4381 gatgtatcgt ggcgcatcgc ctcgaccctg tcgaggaggc gagcctggcc tgctcgcgcc
     4441 ccgccgttcc gaccttggca cgcgagcacc acccgggtgc ccacggacga cgggtcacgc
     4501 cggacggagt atcgggaggg ctaggtgaag aacaagaaag gactactcgc cgccctgggg
     4561 gcgttcacgc tactcatggc gttgacgctc ccggcgagcg caggatcggt ggggaatgca
     4621 tcgggcttcg aggacgacga cggcaacctg gccgtcgaca gcacgttcga ctggaacagc
     4681 ttcgccccca cgacctggga aggcacggcg ccataccggc aatcggacaa ggtcctatcc
     4741 ggttggacgt tcaaaggaat cgaggacgcc caggcgacga acgccgactc ggccttcgcc
     4801 ggcggtacca agcaggacga caactgtccc acggtgaaca ccgggaaggc cccgaacaag
     4861 gacgacctga agcggatcta cctgtccacc aaggtcgtgg gcggccacac ctacctgaac
     4921 ctcgcgtggg tccggatccc gcagaacacg acgtcaccgt ccgcccacat cgggttcgag
     4981 ttcaacaagg gcaccgacgg gacctgcggt ggcagctcca acggtctcgt caagcgcgtg
     5041 gccggcgaca tgctgatcgt gtacgacttc gaaggtggcg cgaccgacac cccggtcatc
     5101 accctccgtc ggtgggtcac ctccggcgcg tgcgaggtgg gcagtgacag cccaccctgc
     5161 tggggaccgg cgaccaacct gaccgacgc
//
LOCUS       DASQQQ010000385         7204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_108998, whole genome shotgun sequence.
ACCESSION   DASQQQ010000385 DASQQQ010000000
VERSION     DASQQQ010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108998"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1632)
                     /locus_tag="VE646_14410"
     CDS             complement(<1..1632)
                     /locus_tag="VE646_14410"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HZD81213.1"
                     /translation="MAITLNRRATERLEQIWKEPPGLKTILESIDHKRLGLRYLYTAF
                     AFFLAGGAEALAMRTQLARPSEQLLSPNMYNQLFTLHGVTMIFFFVIPMGTGFAVYLL
                     PLMLGSRDLAFPRLNNFSYWVFLMAGLFMYSSWVINEVPNAGWFDYVPLASRTYSPGL
                     NIDFYTLGLLFLGVSTTAAAINVVVTFFKMRAPGMTLGRTPLFAWAFLAQSLSIIFAL
                     PSLTAALIFLWLDRNLGFHFYDPGAGGDVVLWQHLFWIFGHPEVYIIFLPAAGVMHSI
                     MSTFTRRSVVGYPVLVLAEMGTAALGFGVWVHHMFAIGLPNLTMSFFAAVSLVIVIPS
                     AIQVLAWVATMLTGRLDLKTPMLFGIAFIWQFVVGGLSGAMFADIPFDQAATDSYMVV
                     AHLHYVLLGASAFPLLAGLYVWMPKFYGRMYHEALGKLSFWLVFVGFNVTFFPMHFQG
                     MAGMPRRIYTYLPGRGWAGYNLAETIGAYILTAGIVAIIANVIWTKKYGPPAPANPWG
                     GETLEWATSSPPPVYDFAQIPIVRSRAPVREQEELR"
     gene            complement(1636..2598)
                     /gene="coxB"
                     /locus_tag="VE646_14415"
     CDS             complement(1636..2598)
                     /gene="coxB"
                     /locus_tag="VE646_14415"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HZD81214.1"
                     /translation="MLVASGCVSNRSNITRPAGSAAHHVANDWTYLIWTSVAVLAIIT
                     ILLLWAVVFRRNRGPNPARNPRGGVWLIVVGGVMLPVMLLSTEWALSNVTLASIADPG
                     KPALTITVIGHQWWWELRYPAPGGGTFTTANEIHVPVGVPVRFVLRSADVNHSFWVPE
                     LAGKTDLIAGRVNGFTTTVTTPGTYYGQCAEFCGLQHAHMLILVVAQPQAAFQSWLAN
                     QAAPAPTPRSPSLQQGEQVFVNGPCSACHTIRGTPADGTVGPDLTHVGSRQRLAANSV
                     PNTPGYLGGWISDSQTIKPGNLMPPIALAPKNLQDVISYLESLK"
     gene            2857..3420
                     /locus_tag="VE646_14420"
     CDS             2857..3420
                     /locus_tag="VE646_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HZD81215.1"
                     /translation="MATTTDRDVIDEVIDDHAQIKRLFAEVESATGTGKRDAFEALMR
                     KLVVHETAEQEVVHPLTRAAHAEGVVDERLSEEKEGERMLAELAGIGPDDAGFDATFT
                     KLKEDVLQHARMEEQHEHPKIRAEIEESRLRQLATAFRAAETAAPTRPHPHGPTSATG
                     NMAVGPIVAIMDRARDAIRDAMRKISA"
     gene            complement(3466..3855)
                     /locus_tag="VE646_14425"
     CDS             complement(3466..3855)
                     /locus_tag="VE646_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZD81216.1"
                     /translation="MNSDREPSDAEYGQLLVFRTELRRFLHWSEEQAHAEELTPAQHQ
                     LLLAIRGHASGSPTVGEVAEVLLLRHHSAVGLVDRAVEAGLVERQRSERDHRVVRLRL
                     TPLGERKLARLGARHLEELRRLRRFGF"
     gene            3877..5151
                     /locus_tag="VE646_14430"
     CDS             3877..5151
                     /locus_tag="VE646_14430"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZD81217.1"
                     /translation="MLRPFSFGDGWLDRFMLGLPRRMVGLIVLVGFGGGAVGAAYLLG
                     LHLLQRVLWPGRWSGAVGFAVLGGVGLFAGLVTRFVGSPGDVELMVDNIHVSGTATGL
                     RTLRSLIPISLLCIASGGAMGPEAPLVQTAGSAASYVGKRWYPKPREARVLTITGMAA
                     AFTVLFGAPIGSAIFALELLHRRGLQYYEALLPAILGSLAGYTIYAGLTGAGLAPVWR
                     LPAPVAISLGDLGWSIVAGAAGAVVAVAFTYTSAALRRAARWLRPELRPVLGGLGLAA
                     LGLLSAYSLTYGEGQVTQIVEHHGTVAFFALAALAKLAGTSITLASEWRGGFIIPLFF
                     IGASLARALHGAFPGSHEAILIAALMVAANVGVTKTPLGSTLVITEMGGIRLLPTTLL
                     AAVVALMLTSEVGLIQTQRERDAIGGPSAGTG"
     gene            complement(5640..6335)
                     /locus_tag="VE646_14435"
     CDS             complement(5640..6335)
                     /locus_tag="VE646_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018889924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZD81218.1"
                     /translation="MRTYGQYCPIARGAEIFAERWTPLILRNLALGCRTFSELHQGVP
                     TMSRTLLSKRLRSLERQGIVGRERYREGRASYVLTRAGEELAELSFALGTWGARWLQV
                     GPEHTDPLVVLWAWRNAVKPERLPKRRVVIRFDLPSGKHRRFWMLFEDGEGEVCLRPP
                     GGDEDMIVKTDPYTLMLVHMGRLSIPEATARGVWTMEGDPVLVRRFPSWGGLSRYAKV
                     RPVRSSSAGRHSA"
     gene            6439..>7204
                     /locus_tag="VE646_14440"
     CDS             6439..>7204
                     /locus_tag="VE646_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZD81219.1"
                     /translation="MSTAPTAPVSTVPPAALSGFRERFRGELLEPGIGAYEEARTIWN
                     GAIDRKPGLIARCRDASDVAAAVRLAADHDLLTSVRSGGHGVGGLALCDHGLVIDLSA
                     MRRIEVDPRARTVRVEAGVLLGELDRATQAHGLAVPSGIVTHTGVAGLTLGGGIGWLM
                     RKHGLTADHLLAAEVVLADATRVRATREEHPELFWALRGGGGNFGVVTSFLFRAHEIG
                     PVVPAGPLLFPIERAEEVMGFYRDWAHQTPDELTTIL"
BASE COUNT         1149 a         2468 c         2523 g         1064 t
ORIGIN      
        1 ccgcagctcc tcctgctcgc ggacgggagc ccggctccgg acgatgggga tctgcgcgaa
       61 gtcgtagacg ggcggtgggg aggaggtggc ccactccagg gtctcgccgc cccaggggtt
      121 cgccggggcg ggcggaccgt acttcttcgt ccagatcacg ttcgcgatga tcgcgacgat
      181 ccccgcggtc aggatgtacg ccccgatcgt ctcggccagg ttgtagcccg cccacccgcg
      241 gcccgggagg tacgtgtaga tccggcgggg catgcccgcc atcccctgga agtgcatggg
      301 gaagaacgtc acgttgaagc cgacgaagac cagccagaag gacagcttgc ccagcgcctc
      361 gtggtacatg cgcccgtaga acttcggcat ccagacgtag agcccggcta gcagggggaa
      421 ggccgacgcc cccagcagga cgtagtggag gtgggccacc accatgtacg agtcggtcgc
      481 ggcctggtcg aacgggatgt ccgcgaacat cgcccccgac aacccgccca ccacgaactg
      541 ccagatgaac gcgatgccga acagcatggg ggtcttcagg tccagtcgcc cggtgagcat
      601 ggtggccacc caggccagca cctggatcgc gctcgggatg acgatcacca ggctcacggc
      661 tgcgaagaag ctcatggtga ggttcggcag cccgatcgcg aacatgtggt ggacccagac
      721 cccgaatccc agcgccgccg tccccatctc ggccagcacc agaaccggat agcccaccac
      781 cgagcggcgg gtgaacgtgg acatgatcga gtgcatcacg ccggcggccg gcaggaagat
      841 gatgtagacc tcggggtggc cgaagatcca gaacaggtgc tgccacagca ccacgtcccc
      901 gccggcgccg gggtcgtaga agtggaaccc caagttgcgg tccagccaca ggaagatcag
      961 cgcggcggtc agcgacggca gcgcgaagat gatggacagc gactgcgcca ggaacgccca
     1021 cgcgaacagc ggggtgcgcc cgagggtcat ccccggcgcc cgcatcttga agaacgtgac
     1081 cacgacgttg atcgcggcgg ccgtcgtgga tacgccgagg aacagcaggc cgagcgtgta
     1141 gaagtcgatg ttcagcccgg gcgagtacgt gcgggaggcc aggggcacgt agtcgaacca
     1201 accggcgttg gggacctcgt tgatcaccca gctcgagtac atgaacaggc cggccatcag
     1261 gaacacccag tagctgaagt tgttcagccg ggggaaggct aggtcccggg agccgagcat
     1321 caggggcaac aggtagacgg cgaagcccgt gcccatgggg atcacgaaga agaagatcat
     1381 cgtcaccccg tgcagcgtga acagctggtt gtacatgttc gggctgagca gttgttcgct
     1441 gggccgtgcc agctgggtcc gcatggcgag cgcctcggcc ccacccgcca ggaagaaggc
     1501 gaacgcggtg tacaggtacc gcagtccgag tcgcttgtgg tcgatcgact ccaggatcgt
     1561 cttcagcccg ggcggctcct tccagatctg ctccaggcgt tcggtggcgc ggcggttaag
     1621 ggtgatagcc atgggctact tcaagctctc caggtagctg atgacgtcct gcaggttctt
     1681 gggggccagg gcaatggggg gcatcaggtt gcccggcttg atcgtctgcg agtccgagat
     1741 ccacccgccc aggtagcccg gagtgttggg gacgctgttc gccgccagcc gctggcggct
     1801 gcccacgtgg gtcaggtcgg gccccacggt cccgtcggcc ggggtgccgc ggatcgtgtg
     1861 acaggccgag cacggcccgt tcacgaagac ctgctccccc tgctggagcg acgggctcct
     1921 aggggtgggc gcgggcgcgg cctggttggc gagccagctc tggaacgcag cctggggctg
     1981 cgccaccacc aggatcagca tgtgcgcgtg ctggagcccg cagaactcgg cgcactgccc
     2041 gtagtaggtc ccgggggtag tcaccgtggt ggtgaagccg ttcacgcgac cggcgatcag
     2101 gtccgtcttg ccggccagct cgggaaccca gaagctgtgg ttcacgtcgg ccgagcgcag
     2161 cacgaaccgc accgggaccc cgaccggcac gtggatctcg ttggcggtgg tgaaggtgcc
     2221 gccgcccggg gccgggtagc gcagctccca ccaccactgg tggccgatca cggtgatcgt
     2281 gagggcgggc ttgccggggt cggcgatgct ggccagcgtg acgttgctga gggcccactc
     2341 cgtggacagc agcatcaccg gcaacatcac gcctcccacc acgatcagcc agaccccacc
     2401 ccgagggttc cgcgccgggt tcgggcccct gttccggcgg aagaccaccg cccacagcag
     2461 caagatcgtg atgatggcga gcacggcgac cgaggtccag atcaggtagg tccagtcgtt
     2521 ggccacgtga tgcgccgccg agccggccgg acgggtgatg ttcgagcggt tggagacgca
     2581 cccggatgcc accagcatca gcaccccgca tcccagcgcg tagggcagac gacaggatcg
     2641 gagcccggag cctcgcacgc agccgctgta cccatgggcc attgggccga aacgagatcc
     2701 gccccgtcgc cccctcggtc cccgtcacgc cttccggagg cgcgttggac ggcccccgtg
     2761 atccgcaccg gcggcccctg gtgtcgccgc cccgggcggc gggtaggcca tggtcgaaca
     2821 gggtgcgaac gacctatcga ccgacaggag tggaccatgg ctacgacgac cgaccgggac
     2881 gtcatcgacg aggtgatcga cgaccacgcg cagatcaagc ggctcttcgc cgaggtggag
     2941 tcggcgaccg gcacgggcaa gcgggatgct ttcgaggcgc tgatgcgcaa gctcgtggtc
     3001 cacgagaccg cggagcagga ggtggtccat ccgctcacgc gcgcggccca cgccgagggg
     3061 gtcgtggacg agcgcctctc cgaggagaag gagggcgagc ggatgctggc cgagttggcg
     3121 ggaatcggcc ccgacgacgc cgggttcgat gccacgttca ccaagctcaa ggaagacgtc
     3181 cttcagcacg cgaggatgga ggagcagcac gagcacccga agatccgggc ggagatcgag
     3241 gagagccggc tccgccagct ggccaccgcc ttccgggcgg ccgagacggc ggcccccacc
     3301 cgaccgcatc cccacgggcc gacctcggcc acgggcaaca tggcggtggg gccgatcgta
     3361 gcaatcatgg accgggcgag ggacgcgatc cgcgacgcga tgcggaagat cagcgcctga
     3421 cgaggaccat ccctcgccgg acgaccgacc ggcgctgctc gtcggtcaga agccgaacct
     3481 gcgcaggcgc cgcagctctt ccaggtgacg cgcgcccagt cgcgccaact tccgctcacc
     3541 gagcggggtg aggcggagcc ggaccacccg gtgatcccgc tcgctgcgct gccgctctac
     3601 caggccggcc tccaccgccc ggtcgacgag ccccaccgcg ctgtggtggc ggagcaacag
     3661 cacctcggcg acctccccta ccgtgggcga gcccgaggca tggcccctga tggccaagag
     3721 gagctgatgc tgcgccggcg tgagctcctc ggcatgcgcc tgctcctcgc tccagtgcag
     3781 gaaccggcga agctcggtac ggaacacgag cagctgcccg tactcggcgt cggagggttc
     3841 tcggtccgag ttcataggtc gtattacgat actatcgtgc ttcgaccttt cagcttcggt
     3901 gatgggtggc tggaccggtt catgctcggc ctgccgcgcc gcatggtggg cctgatcgtc
     3961 ctcgtggggt tcggcggcgg cgccgtcggc gccgcgtacc tcctgggcct ccacctgctg
     4021 cagcgggtcc tgtggccggg gcgctggtcg ggagccgtgg ggttcgccgt gttgggtggc
     4081 gtgggcctgt tcgccgggct cgtcacgcgc ttcgtgggca gcccgggcga cgtggagctg
     4141 atggtggaca acatccatgt gtccggcacc gcgaccggcc tgcgcacgct ccggtccctg
     4201 atccccatct ccctcctgtg catcgcctcg ggtggggcga tggggccgga ggcgccgctg
     4261 gtccagacgg cggggtcggc cgcctcctac gtgggcaagc gctggtaccc gaagccgcgg
     4321 gaggcacgcg tgctcaccat caccgggatg gcggcggcct tcacggtgct cttcggcgct
     4381 ccgatcggct ccgcgatctt cgctctggag ctgctccacc gccgcgggct ccagtactac
     4441 gaggccctgt tgccggcgat cctgggctcg ctcgccgggt acacgatcta cgccggcctg
     4501 accggcgccg ggctcgcgcc cgtgtggcgc ctgccggccc cggtcgcgat ctcgctggga
     4561 gacctcggct ggtcgatcgt cgctggggcg gccggcgcgg tggtggcggt ggccttcacc
     4621 tacacgagcg cggctctgcg ccgggcggcc cgatggctcc gccccgagct ccgcccggtg
     4681 ctcgggggcc tggggctggc cgcactcggc ctgctgtccg cctactcgct cacctacggc
     4741 gagggccagg tcacccagat cgtggagcac cacgggacgg tggcgttctt cgcgctggcc
     4801 gcgctggcca agctcgccgg gacctcgatc acgctggcca gcgagtggcg aggaggcttc
     4861 atcatcccgc tgttcttcat cggtgcctcg ctggcgcggg ccctgcacgg ggccttcccc
     4921 ggcagccacg aggccatcct catcgcggcc ctgatggtgg ccgccaacgt cggggtcacc
     4981 aagacccctc tgggatccac gctcgtgatc accgagatgg gcggcatccg gttgctgccc
     5041 accaccctgc tggccgccgt ggtggcgctc atgctcacca gcgaggtcgg cttgatccag
     5101 acccaacggg agcgcgacgc gatcggcggg ccgtcagcgg ggacgggatg actcttcgtc
     5161 gcatccgtgc taccggatcg cccgcagcgc gagggacggc ttgtgccgga gactccgctc
     5221 ccgctcgggc cggtctggat ccgacgtgtt cgaggaggcg cttcgcgaac gtccgtggac
     5281 gggaatcgcg agcggactat cgctcgctat cagactccag atgcccttca cgttgctgag
     5341 actccgctcc atcatcgccc gaacgaactc cgtcttgtcg ggctccatct gggggagctc
     5401 cgtcgtcacg gtgaggagtg ttcgcccttt cctctgagcc tcgaacgtgg cccgcccaaa
     5461 catgtccctg ttcccgtcgt ggatcgatag gcgaaccggc gcgggcaaca gcgccaacgc
     5521 accagaccct gcccacatga ggtgggagca gcgaccgagg gccgggcagg agcagcacca
     5581 ccggttccac caccccgttc gtctgggcat gcgagccggc gcgccaagca gaacggcggt
     5641 caggcagagt gacgaccggc ggacgacgag cggacgggcc gcaccttcgc gtaccgggaa
     5701 agcccacccc aggaagggaa ccgacgaacg aggacagggt cgccctccat cgtccagacg
     5761 ccccgtgccg tcgcctcagg gatcgacagg cgtcccatgt gcacgagcat cagcgtgtac
     5821 ggatcggtct tcacgatcat gtcctcgtcg ccgccgggcg gcctcaggca cacctcgccc
     5881 tcgccgtcct cgaacagcat ccagaaccgt cgatgcttcc ccgacgggag gtcgaagcgg
     5941 atgaccactc gccgcttcgg aagccgctcg ggcttgaccg cgttcctcca ggcccacagc
     6001 acgacgagcg ggtcggtgtg ctcgggtccg acctgtagcc acctcgcgcc ccaggtcccc
     6061 agggcgaatg agagctccgc cagctcctcc cccgcccggg tgaggacgta ggacgcgcgc
     6121 ccctcccggt accgctcgcg cccgacgatc ccctgccgct cgagcgaacg caagcgcttg
     6181 gagagcagcg tcctggacat ggtgggcacg ccctgatgca gctccgagaa cgttcggcat
     6241 ccgagggcca ggttccgcag gatgaggggc gtccaccgct ccgcgaagat ctccgcgccc
     6301 ctggcgatcg gacagtactg accgtaggtc cgcatcctcg catcctgtcc ggtcggcggg
     6361 ttccatacaa gtccactttc tggactcgac atgagcccgc tcgccgggga ccatcggacc
     6421 agcagcgagg aggtcccgat gagcaccgct cccaccgccc ccgtctcgac ggtcccgccg
     6481 gccgcgttgt ccgggttccg cgaacggttc cgaggagagc tcctggagcc agggatcggg
     6541 gcgtacgagg aagcgaggac gatctggaac ggggcgatcg atcgcaagcc cggactgatc
     6601 gccaggtgcc gggatgcctc cgacgtcgcg gcagccgtgc gtctggccgc agatcacgac
     6661 ctcctcacgt cggttcgaag tggcgggcac ggagtgggcg gcctcgccct ctgcgaccac
     6721 gggctggtga tcgatctgtc cgcgatgcga cggatcgagg tcgacccacg cgcccggacc
     6781 gtgcgagtgg aggcgggcgt gctgctcggc gagctggacc gggcgaccca ggctcacggc
     6841 ctggccgttc cctcggggat cgtcacccac acgggggtcg ccggcctcac gctcggtgga
     6901 gggatcgggt ggctgatgcg caagcacgga ctgaccgccg accacctgct cgcggccgag
     6961 gtcgtgctgg ccgacgcgac gagggtcagg gcgacgaggg aggagcaccc cgagttgttc
     7021 tgggcgctcc gcggaggcgg cgggaacttc ggcgtcgtga cctcgttcct gttccgtgcg
     7081 cacgagatcg ggccggtcgt accggccggc ccgctgctgt tcccgatcga gcgcgccgag
     7141 gaggtgatgg gcttctatcg agactgggcg caccagaccc cggatgagct gaccaccatc
     7201 ctca
//
LOCUS       DASQQQ010000386         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_109471, whole genome shotgun sequence.
ACCESSION   DASQQQ010000386 DASQQQ010000000
VERSION     DASQQQ010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109471"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1242
                     /locus_tag="VE646_14445"
     CDS             166..1242
                     /locus_tag="VE646_14445"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HZD81220.1"
                     /translation="MRELLARWGRDEEAWSDLRAGALTGLLSSAYSTLFVQFLARDTG
                     RDPLVDWMQVGTVPLRNRAIRSRPGPVGVSAGVLAHQFADFFWAVGFFGTIIPRIPRR
                     RRTAFLLASAAPWAAATAAAEYYLALPWLQPILPMQVPYWTAMGVHLSSAAAYPLFML
                     FGEGDRTAGRRAAALLAASVPIMAFLDARSRSRRGLRWAIRGQEEDRRFLHMMTGHHR
                     TGLRLAELAAERAAAPDLRALGRLMAAEHDAEIRLMADWWATWFGGEVQPLSSTERDA
                     MPGMPLRTDVDRLARMDGRSFDHRFLELMVPHHLGAIRMSERKLSEPGDVRLRLLARN
                     IRHSQLRQIDRMESSRWIEDDHRG"
     gene            1505..1765
                     /locus_tag="VE646_14450"
                     /pseudo
     CDS             1505..1765
                     /locus_tag="VE646_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092156.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress.; incomplete; partial
                     in the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
     gene            1888..>2208
                     /locus_tag="VE646_14455"
     CDS             1888..>2208
                     /locus_tag="VE646_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZD81221.1"
                     /translation="MTGWTTPRARARWLLLGVAAALALLLVVGRLQHGDLAAHRPAPA
                     PVAPTTSARPLPVTATIGLGELSGPQFASWSQVMVGEGRLWVVVDGVLVGVDPRRLRT
                     VVRVR"
BASE COUNT          272 a          725 c          854 g          357 t
ORIGIN      
        1 gttctccatc ggcgagcacg tagggacaca ccgtcctcgg tccgcaccct gaactcgtcg
       61 taggaggcca tcatcgggcg cctacccatc ctggtggctt ccacaccgcg gatcggtggc
      121 atgggtcgtc ctcaggttgg ggatgaacag tggtgcatgc gatccatgag ggagctcctg
      181 gcgcgctggg ggcgcgatga ggaagcgtgg agcgatctgc gggcgggcgc gctgaccggc
      241 ctgctgtcct ccgcctactc cacgctgttc gtgcagttcc tggcccggga cacgggccgc
      301 gacccgctgg tggactggat gcaggtgggc accgtcccgc tccggaaccg agcgatccgc
      361 tcgcgccctg gaccggtcgg ggtctcggcg ggggtcctcg cgcatcagtt cgccgacttc
      421 ttctgggcgg tggggttctt cgggacgatc atcccccgga tccctcggcg ccgccggacg
      481 gcgttcctgc tggcctcggc ggcgccctgg gccgccgcca ccgccgccgc cgagtactac
      541 ctggctctgc cctggttgca gccgatcctg ccgatgcagg tcccatactg gaccgccatg
      601 ggggtgcacc tcagctcggc ggccgcctac ccgctgttca tgctgttcgg ggagggggat
      661 cgaacggcgg ggcggagggc ggcggcgctg ctggccgcgt cggtcccgat catggcattc
      721 ctcgacgccc gctcacggag ccggcgcggg ctgcggtggg cgatccgggg ccaggaggag
      781 gaccgccggt tcctgcacat gatgacgggc caccaccgga ccggactgcg gctggccgag
      841 ctggccgccg agcgggccgc ggcccccgat cttcgtgctc tggggcgctt gatggcggcc
      901 gagcacgacg cggagatccg gctgatggcg gactggtggg cgacgtggtt tgggggcgag
      961 gtccaaccac tgagctcgac agagcgcgac gcgatgccgg gaatgcccct tcggacggac
     1021 gtcgaccgcc tggcgcggat ggacgggcga tcgttcgacc accggttcct ggagctcatg
     1081 gtcccccatc acctgggggc gatccggatg agcgagcgga aactctccga gccaggcgac
     1141 gtccgcctgc ggctcctggc ccgcaacatc cgccactcac agctccgcca gatcgatcgg
     1201 atggagtcgt cccgctggat cgaggacgac catcgaggat gacccgcggg cacgagagtg
     1261 atcagctggc cgggatccac aggcagggaa gtgcgccccg ggagggattc gagccccgac
     1321 cttctgatcc gtagtcgcaa gacaggcgtc tgtctgctgc ggtggagcga cgttatcgca
     1381 ggtcacgacg gtgccgccgc ctggcgtccg cggtcgaggt tggccatctt cggcgcagta
     1441 gatggccaat cggatggcca agcgttgctc agccagcttc aacctcgcgg gggtgcttgg
     1501 cgccgcgcgg gaggcggcgt ggcgggatgc tcgcggtcgg gccgagcagt atgccgcgct
     1561 ggccggtgtc gcgctgggcc gggtcttgcg gatcgacgag gtgggcggcg cgcgacccgt
     1621 gctgccgctg gcggggcgct acgaggcgca ggctgctgcc ggcccggccg tggaggtggg
     1681 tgaggcggag gtgggggcaa gggtggccgt cacgtgggcg ttgctggacc ctccgctgcc
     1741 ccccgccggt gagcgctcgt ggtgatcgcc gttgaggttc acacaccgtc tctgtgtcgt
     1801 cgggccgtcg gtccccggcg atccggccgc cgctgcgggc agggtaggat gaccaggctg
     1861 aacggtttgc ctgggcaagg gtgggtgatg accggctgga cgacccccag ggcacgcgcg
     1921 cgctggctgc tgctgggggt cgccgcggca ctggcgctgc tgctggtggt tggtcgcctt
     1981 cagcacggtg acctcgcggc ccatcggccc gcaccggcgc cggtggcgcc gaccacgagc
     2041 gcccgcccgc tgccggtcac ggccaccatc gggctgggcg agttatctgg gccgcagttc
     2101 gccagctggt cgcaggtcat ggtcggggag ggcaggctgt gggtggtggt ggacggggtc
     2161 ctggtcggtg tcgatccgag gcgcctccgc acggtggtca gggtccgg
//
LOCUS       DASQQQ010000387         2933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_109594, whole genome shotgun sequence.
ACCESSION   DASQQQ010000387 DASQQQ010000000
VERSION     DASQQQ010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109594"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1861)
                     /locus_tag="VE646_14460"
     CDS             complement(149..1861)
                     /locus_tag="VE646_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019985008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HZD81222.1"
                     /translation="MPSGTGAGVSDRRLESELSVVLGINAVFHDPAAALVVDGRIVAA
                     AEEERFSRRKHGKLPVPFSTWELPERAAAWCLRSAGLDPEHLDAVGYSYDPSALPDPG
                     PDITARDWEGLRTLFAHRAPLFLRAALPGLDPAIVRFVPHHVAHAASAYLAAPTDRSA
                     VMVLDGRGERASYLSGRARDGRLEVVAVQPLPHSLGLLYEEVTEHLGFRRASDEYKVM
                     ALASYGDPAFAPEMGRAVRTTGDGRFTVRFPEWSSLVKPRRPGDPWSAEHADLAASVQ
                     RRLEEVLLELARWLHHRTGESTLTMAGGVALNCVANSRLAQEGPFEQVWVQPAAGDAG
                     TALGAALYLARELGDRPEPFSTPALGRGWSERVLAERLEEAAVPFERPSDLADQVAAI
                     LAEDGVVAWFQGRSEWGPRALGHRSLLADPRRPENLDRLNRIKGREEFRPVAPLVLTE
                     RASRVFEGGPFPSPYMLFTHRVRPEWRERIPAVVHVDGTSRIQTVDAEQEPLVAAVLR
                     AFERRTGVPVVVNTSLNTAGRPIVDDPRDALECFGSAPIDALAIGPYLVRRASVFARS
                     SDAA"
     gene            1958..>2933
                     /locus_tag="VE646_14465"
     CDS             1958..>2933
                     /locus_tag="VE646_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZD81223.1"
                     /translation="MAAARDDWGPGRALVTGGAGFLGSHLCERLLDEGAEVICLDNLS
                     TGARDNVAHLIGRAGFTLWHADLAAPIAPSDGVAPFGRERPVDTVLHLASPASPRDYL
                     RMPIETLRVGSTGTFRALELARELRARFLLASTSEVYGDPLVHPQPEGYRGNVDPVGP
                     RSVYDEAKRFSEAATAAFARQAGLEVRIARIFNTYGPRMRPDDGRVIPTFVHQALSGR
                     PFTINGDGEQTRSLCYVSDMVEGLWRLLRSDVRVPVNLGNPVEHSVLEVAAAVARTAG
                     VEPDYRFNAPMSDDPRLRRPDIGRAQQLLGWRPKVSLADGLRITMDWFR"
BASE COUNT          411 a         1102 c         1036 g          384 t
ORIGIN      
        1 gaccgtaccc tcggccagcg tcgtggcggc gtcggcccgg tcgtcgacga tcaggatccg
       61 cccaggcagg gggctctcgg cgcggcccag cgcctcgagg agccgggcca aggacggtcg
      121 gcccacggtg gggacgacca ggtcgatctc aggctgcatc ggaggaccgg gcgaacaccg
      181 aggcccgccg gaccagatag gggccgatgg ccagggcgtc gatcggcgcc gatccgaagc
      241 actcgagggc gtcccgggga tcgtccacga tcggtcggcc agccgtgttc aggctggtgt
      301 tcaccaccac cgggaccccg gtccgacgct cgaaggcccg gagcaccgcc gccaccagcg
      361 gctcctgctc ggcgtccacg gtctggatgc gcgaggtccc gtccacgtgc accacggcgg
      421 ggatgcgctc cctccactcg ggacggaccc ggtgggtgaa cagcatgtac gggctgggga
      481 acggtccccc ctcgaagacc cggctcgctc gctcggtcag gacgaggggc gccaccggac
      541 ggaactcctc gcgtcccttg atccggttca gccggtccag gttctcgggc cgtcggggat
      601 ccgccagcag gctccggtga ccgagcgccc ggggacccca ctcgctcctc ccctggaacc
      661 aggccaccac cccgtcctcg gccaggatcg ccgcgacctg gtcggccagg tcggagggcc
      721 gctcgaacgg cacggcggcc tcctccagcc gttccgccag gacccgctcg gaccatcccc
      781 gcccgagcgc cggggtgctg aagggttccg gacggtcacc gagctcacgg gccaggtaca
      841 gggcggcgcc cagcgcggtc ccggcgtcgc ccgcggccgg ctggacccac acctgctcga
      901 agggcccctc ttgcgccagc cggctgttgg cgacgcagtt cagcgccacc ccgcccgcca
      961 tggtcagcgt ggattccccc gtcctgtggt ggagccaacg ggccagctcc agcaggacct
     1021 cttcgagccg acgctgcacg ctggccgcca gatcggcgtg ctccgcgctc cagggatcgc
     1081 cggggcgccg gggcttcacc agcgaggacc actcggggaa ccggaccgtg aaccgcccgt
     1141 cgccggtggt tcggaccgcc cgccccatct ccggggcgaa cgcgggatcc ccgtaggagg
     1201 cgagggccat caccttgtac tcgtcggagg ctcggcggaa ccccaggtgc tcggtgacct
     1261 cctcgtacag caggcccagg gagtgcggca ggggctggac ggccacgacc tccagccgtc
     1321 cgtcccgggc gcggcccgac aggtaggagg cacgctcccc ccgaccgtcg agcaccatga
     1381 ccgcggagcg gtccgtgggc gccgcaaggt acgcggaggc ggcgtgcgcc acgtggtgag
     1441 gcacgaaccg cacgatcgcc gggtccagcc cgggcagcgc ggcccgcagg aacagcggcg
     1501 cccgatgggc gaacagcgtg cgcagcccct cccagtcccg ggcggtgatg tcggggcccg
     1561 ggtcgggcag cgcgctggga tcgtacgagt acccgacggc atcgaggtgc tcgggatcca
     1621 accccgccga gcgcaggcac cacgccgcag cccgctccgg cagctcccac gtggagaagg
     1681 gcaccgggag cttcccgtgc ttacggcgac tgaaccgctc ctcctcggcc gccgccacga
     1741 tgcgtccgtc gaccaccagc gccgccgccg ggtcgtggaa caccgcgttg atccccagga
     1801 ctacggacaa ctcactctcc agtcgccggt cggatacgcc ggccccggtc cccgatggca
     1861 tgccggctcg cttggcgcta cccgggccca tgccacgaca aacgctgctt gcacgccggt
     1921 ttcgagccgc gcgggaccgg gcatgcactt gcctgagatg gcagcggcgc gcgacgattg
     1981 gggtcccggc cgcgccctcg tgaccggcgg cgccggcttc ctcggttcgc acctgtgcga
     2041 gcgactcctg gacgagggcg ccgaggtcat ctgtctggac aacctctcca ccggcgctcg
     2101 cgacaacgtg gcccatctga tcggtcgggc cggcttcacg ctgtggcatg ccgacctggc
     2161 ggcgccgatc gctccatccg acggggtggc ccccttcgga cgcgagcgtc cggtggacac
     2221 ggtgctgcac ctcgcctctc cggccagccc ccgggactac ctccggatgc cgatcgagac
     2281 gctccgggtc ggctcgacgg ggaccttccg ggccctggag ctcgcgcggg agctccgcgc
     2341 ccggttcctc ctcgcctcca cctccgaggt ctacggggac cccctggtcc atccgcagcc
     2401 ggagggctac cggggcaacg tagacccggt cggaccgcga tccgtgtacg acgaagccaa
     2461 gcgcttctcg gaggccgcga ccgcggcgtt cgcccggcag gccggcctgg aggtgcggat
     2521 cgctcggatc ttcaacacgt acgggccccg gatgcgcccc gacgacgggc gggtgatccc
     2581 gaccttcgta caccaagcgt tgagcggacg gccgttcacg atcaacggcg acggcgagca
     2641 gacccggtcg ctctgctacg tgagcgacat ggtggagggc ctgtggcgcc tcctgcgctc
     2701 ggacgtgcgg gtgccggtga acctgggcaa ccccgtcgag cactcggtgc tcgaggtcgc
     2761 cgccgcggtg gctcggaccg ccggggtgga gcccgactac cgcttcaacg ccccgatgag
     2821 cgatgacccg cgcctccgcc gtcccgacat cgggcgggcc cagcagctgc tgggatggcg
     2881 gccgaaggtg agcctggccg acgggctgag gatcacgatg gactggttcc gta
//
LOCUS       DASQQQ010000388         3321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U10861
            k141_248026, whole genome shotgun sequence.
ACCESSION   DASQQQ010000388 DASQQQ010000000
VERSION     DASQQQ010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946338
            Sequence Read Archive: SRR5916120
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 16:39:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,897
            CDSs (total)                      :: 2,859
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 9 of 45
            Pseudo Genes (incomplete)         :: 41 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 6 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248026"
                     /isolate="SMAG_U10861"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /lat_lon="13.74768 S 141.50618 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VE646_14470"
     CDS             <1..513
                     /locus_tag="VE646_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HZD81224.1"
                     /translation="GIVTSTSLRIGGDELDESIINFVKKEYSLLLGERTAEAIKLAIA
                     SVYPMQEELIAEIPGRDLVTGLPKTIQITDSEIRQAIEEPVTSIIDSIKATLDRTPPE
                     LAGDIMHKGIVLTGGGALLRGLDRRLSHETGMPVHIAENPLSSVAIGSGRCLEEFEVL
                     QRVLVSPSRR"
     gene            520..1377
                     /gene="mreC"
                     /locus_tag="VE646_14475"
     CDS             520..1377
                     /gene="mreC"
                     /locus_tag="VE646_14475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HZD81225.1"
                     /translation="MALPNRARSTRLLVVALVSICLATITLDYREGDHGPLAAGGRAA
                     ITVIGPLQETVSKVTHPVGNFFSTLVRLPAIRRENELLKERVAALESQIGTAVSDERR
                     LHELEALLDVQQSLGPNVRTVGAQVIANGASNFEWTITIDKGSSDGVRVDDPVVAAGK
                     QLVGHVVRVGPDASMVQLLVDPDSAVAGRLETSGKTGLVTGQGNQDMRMGLIDTTTDV
                     QAGEQVVTAAYRIPGVAQSLYPPGVLIGTVSRVLPDEAALEKYITVSPAVDVSSLDIV
                     LVVLPDGGG"
     gene            1389..1895
                     /gene="mreD"
                     /locus_tag="VE646_14480"
     CDS             1389..1895
                     /gene="mreD"
                     /locus_tag="VE646_14480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HZD81226.1"
                     /translation="MRRTLSLAAIIVTALLLQTTVFAEVRLLGARPELMYLVTILFAM
                     LEGPSSGAVTGFAGGMAQDFLLNQAKGITALTLTLLGYAIGMLRQYIVSPSPLLPVFL
                     VAGGTFVGVLFYGIVAFLLGQLDTSWLYLVRTSALSGLYNAILTPLFLPILRRLAEGS
                     RTRRVIRW"
     gene            1903..>3321
                     /locus_tag="VE646_14485"
     CDS             1903..>3321
                     /locus_tag="VE646_14485"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZD81227.1"
                     /translation="MTDGRIGLRLKVLALLVTFMFAALTTRLWFLQVLAFEQNRDSAN
                     RNAVRTVEVPAPRGRILDDTGRTVLVGNRMSLVVTVNRQEAGDRLEQTLFDLSKVLGI
                     RPTELAARADSKLYYVYQPVPVAIGVSKRVDFYISEHRKMFPGVEVIEEPVRTYPFGD
                     LAAHVLGYLGQISESQLKEPSFSGYAAGDLVGVNGVEAEYEHDLRGTKGVLKYRVNSA
                     GENLGLIGRLAPQPGDDLVLTIDQRLQDLAEQSLKLGLEYAHGIYDSSSAKDLSANAG
                     AVVVMDPTTGAIKAMASFPTYDPSLFTRSMPEKEYKRRFGGATDFPLLNRAVQGQYPP
                     GSTFKPFVLLSALHRGLVSTSQGYDCPPTWSVPEDPFHQVFHNWTTANLGVMSLAQAL
                     WDSCDTVFYPIGYEYWRIFYPPTDPPKEPLQRDLRSDGFGQVVRVDLPGAGPYPGRVP
                     DAEWKAEVNHANPKAFPEGDWFP"
BASE COUNT          510 a         1125 c         1147 g          539 t
ORIGIN      
        1 ggcatcgtca cctcgaccag cctgcggatc gggggcgatg agctcgacga gtccatcatc
       61 aacttcgtga agaaggagta ctcgctgctg ctgggcgagc gcaccgcgga ggccatcaag
      121 ctggccatcg cctcggtcta ccccatgcag gaggagctga tcgccgagat cccggggcgg
      181 gacctggtca ccggcctgcc gaagacgatc cagatcacgg acagcgagat ccgccaggcc
      241 atcgaggagc cggtaacctc catcatcgac tcgatcaagg ccaccctgga ccgcacgccc
      301 cccgagctgg ccggggacat catgcacaag ggcatcgtgc tgacgggagg cggtgcgctc
      361 ctgcgtgggc tggaccggcg cctgagtcac gagacgggca tgccggtcca catcgcggag
      421 aacccgctct cgtccgtggc catcggatcc ggacggtgcc tggaggagtt cgaggtcctc
      481 cagcgggtcc tggtgagccc ctcccgaagg tagggcctcg tggccctccc gaaccgcgcc
      541 aggagcaccc ggctgctcgt ggtggccctc gtctcgatct gcctagccac catcacgctg
      601 gactaccggg agggcgacca cggaccgctg gccgcgggcg gccgagcagc catcaccgtg
      661 atcggcccgc tccaggagac cgtctccaag gtcacccacc ccgtggggaa cttcttctcc
      721 acgctggtcc gcctcccggc gatccgccgg gagaacgagc tgctcaagga gcgggtcgcc
      781 gcgctcgagt cgcagatcgg gacggccgtc tccgatgagc gccggctcca cgagctggaa
      841 gccctgttgg acgtgcagca gagcctcggt cccaacgtgc ggacggtggg cgctcaggtc
      901 atcgccaacg gcgcctcgaa cttcgaatgg accatcacga tcgacaaggg gtccagcgac
      961 ggcgtgcggg tggacgaccc ggtggtcgcg gccggcaagc aactcgtggg ccatgtggtc
     1021 cgggtgggac cggacgcctc catggtccag ctgctggtgg atcccgattc ggcggtggcc
     1081 ggccggctgg agacctccgg caagaccgga ctggtcaccg gtcagggcaa ccaggacatg
     1141 cggatgggcc tgatcgacac caccacggac gtgcaggcgg gagagcaggt ggtcacggcc
     1201 gcttacagga tccccggggt ggctcagagc ctctacccac cgggggtgct gatcggcacg
     1261 gtgtcccggg tcctcccgga tgaggcggcc ttggagaagt acatcaccgt gagcccggca
     1321 gtggacgtgt cgagcctcga catcgtgctg gtggtccttc ccgatggcgg cgggtagcct
     1381 gacggtccgt gcgccgcacg ctgtccctcg ccgccatcat cgtgaccgcc ctgcttctcc
     1441 agaccaccgt gttcgcggag gtccggctgc tgggtgcccg gcccgagctc atgtacctgg
     1501 tcaccatcct gttcgcgatg ctggaggggc cttcgtccgg ggccgttacc ggcttcgccg
     1561 ggggcatggc gcaggatttc ctcctgaacc aggccaaagg catcacggcc ctgaccctga
     1621 ccttgctcgg gtacgcgatc gggatgctgc gccagtacat cgtgagtccc tcgccgctcc
     1681 tgccggtctt cctggtcgcg gggggaacct tcgtcggcgt gctcttctac gggatcgtgg
     1741 cgttcctgct cgggcagctg gacacctcct ggctgtacct ggtccggacc tcggcgctgt
     1801 cgggcctgta caacgcgatc ctgacgccgc tgttcctgcc gatcctgcgc cgactggcgg
     1861 agggctctcg cacccgccgc gtgatccggt ggtgaggagc cgatgacgga cgggcggatc
     1921 gggctgcgtc tgaaggtgct ggcgctgctg gtgacgttca tgttcgcggc cctcaccacc
     1981 cggctgtggt tcctccaggt gctggccttc gagcagaacc gggactcggc gaaccgcaac
     2041 gcggtccgga cggtcgaggt gcccgccccc cggggtcgga tcctcgacga caccggacgc
     2101 acggtcctgg tggggaaccg gatgagcctg gtcgtgaccg tgaaccgcca ggaggccggt
     2161 gatcggctgg agcagaccct gttcgacctc tccaaggtcc tggggatccg acccaccgag
     2221 ctggcggccc gtgccgacag caagctctac tacgtgtacc aaccggtccc cgtggcgatc
     2281 ggtgtctcca agcgcgtgga cttctacatc tccgagcacc ggaagatgtt ccccggcgtg
     2341 gaggtgatcg aggagcccgt gcgcacctac ccgttcgggg atctggccgc ccacgtgctc
     2401 gggtacctgg ggcagatctc ggagtcccag ctgaaggagc cgtccttctc cggctatgcg
     2461 gccggtgatc tggtgggggt gaacggcgtc gaggccgagt acgagcacga cctccggggc
     2521 acgaaggggg tgctgaagta ccgggtgaac tcggccggtg agaacctcgg cctgatcggg
     2581 cgcctggccc cgcagcccgg cgacgatctg gtcctcacga tcgaccagcg cctgcaggac
     2641 ctggcggagc agagcttgaa gctgggcctg gagtatgccc acgggatcta cgactcgagc
     2701 tcggccaagg acctgtcggc caacgcgggc gccgtggtgg tgatggaccc gaccaccggg
     2761 gcgatcaagg ccatggcctc gttccccacc tacgatccgt ccctgttcac gaggagcatg
     2821 ccggagaagg agtacaagcg acggttcggg ggtgctacgg acttccccct cctgaaccgg
     2881 gcggtccagg gccagtaccc gccggggtcc acgttcaagc ccttcgtgtt gctctcggcg
     2941 ctccaccggg gattggtgtc gacgtcgcag ggatacgact gcccgccgac gtggtcggtc
     3001 ccggaggacc ccttccacca ggtcttccac aactggacca ccgcgaacct cggcgtgatg
     3061 agcctggcgc aggcgctgtg ggactcgtgc gacacggtct tctacccgat cgggtacgag
     3121 tactggcgga tcttctaccc ccccaccgac ccgcccaagg agccgctgca gcgcgatctg
     3181 cggtccgacg gcttcggcca ggtggtccgg gtcgacctgc cgggggccgg tccttacccg
     3241 gggcgggtgc ccgatgccga gtggaaggcc gaggtgaacc acgcgaaccc gaaggcgttc
     3301 ccggagggcg actggttccc c
//
